LOCUS       DANBWH010000001        52949 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.001, whole genome shotgun sequence.
ACCESSION   DANBWH010000001 DANBWH010000000
VERSION     DANBWH010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 52949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52949
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.001"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            88..2163
                     /gene="lip2"
                     /locus_tag="PPY92_000001"
     CDS             88..2163
                     /gene="lip2"
                     /locus_tag="PPY92_000001"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip2/Geh"
                     /protein_id="HDJ2624034.1"
                     /translation="MLRGQEERKYSIRKYSIGVVSVLAATMFVVSSHEAQASEKTPTS
                     NAAAQKETLNQPGEQGNAITSHQMQSGKQLDDMHKENGKSGTVTEGKDTLQSSKHQST
                     QNSKTIRTQNDNQVKQDSERQGSKQSHQNNATNNTERQNDQVQNTHHAERNGSQSTTS
                     QSNDVDKSQPSIPAQKVLPNHDKAAPTSTTPPSNDKTAPKSTKAQDATTDKHPNQQDT
                     HQPAHQIIDAKQDDTVRQSEQKPQVGDLSKHIDGQNSPEKPTDKNTDNKQLIKDALQA
                     PKTRSTTNAAADAKKVRPLKANQVQPLNKYPVVFVHGFLGLVGDNAPALYPNYWGGNK
                     FKVIEELRKQGYNVHQASVSAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGK
                     TYKGIMPNWEPGKKVHLVGHSMGGQTIRLMEEFLRNGNKEEIAYHKAHGGEISPLFTG
                     GHNNMVASITTLATPHNGSQAADKFGNTEAVRKIMFALNRFMGNKYSNIDLGLTQWGF
                     KQLPNESYIDYIKRVSKSKIWTSDDNAAYDLTLDGSAKLNNMTSMNPNITYTTYTGVS
                     SHTGPLGYENPDLGTFFLMDTTSRIIGHDAREEWRKNDGVVPVISSLHPSNQPFINVT
                     NDEPATRRGIWQVKPIIQGWDHVDFIGVDFLDFKRKGAELANFYTGIINDLLRVEATE
                     SKGTQLKAS"
     gene            complement(2405..3232)
                     /locus_tag="PPY92_000002"
     CDS             complement(2405..3232)
                     /locus_tag="PPY92_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDJ2624035.1"
                     /translation="MTEIKYKVITKDAFALPYTIIKAKNQPTKGVIVYIHGGGLMFGK
                     ANDLSPQYIDILTEHYDLIQLSYRLLPEVSLDCIIEDVYASFDAIQSQYSNCPIFTFG
                     RSSGAYLSLLIARDRDIDGVIDFYGYSRINTEPFKTTNSYYAKIAQSINETMIAQLTS
                     PTPVVQDQIAQRFLIYVYARGTGKWINMINIADYTDSKYNIAPDELKTLPPVFIAHCN
                     GDYDVPVEESEHIMNHVPHSTFERVNKNEHDFDRRPNDEAITIYRKVVDFLNAITMV"
     gene            complement(3305..4504)
                     /locus_tag="PPY92_000003"
     CDS             complement(3305..4504)
                     /locus_tag="PPY92_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDJ2624036.1"
                     /translation="MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNASTKEGYITKADL
                     AYAARRSNSAGMQVTGAAYIEPYGKLFEYGFNIDHDACIPGLTNMASTMKQHGSLAII
                     QLAHAGRFSNQAILNFGKVYGPSPMTLHSPIEHVVIAMSHEKINSIIQQYRDATLRAI
                     KAGFDGVEISIAQRLLIQTFFSTFSNRRTDHYGADSLKNRARLCLEVMRAVQEVIDKE
                     APDNFILGFRATPEETRGSDLGYTIDEFNQLIDWVMDVSNIQYLAIASWGRHIYQNTS
                     RTPGDHFGRPVNQIVYEHLAGRIPLIASGGINSPESALDALQHADMVGMSSPFVTEPD
                     FVHKLAEQRPHDINLEFSMADLEDLAIPHAAFKDIVKMMDYGEGLKKHTRDALRQLEQ
                     NYHDSSS"
     gene            4600..4692
                     /locus_tag="PPY92_000004"
     CDS             4600..4692
                     /locus_tag="PPY92_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624037.1"
                     /translation="MENIFKIELMNGICRSENMNMKKKNKGEKI"
     gene            4806..5807
                     /locus_tag="PPY92_000005"
     CDS             4806..5807
                     /locus_tag="PPY92_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDJ2624038.1"
                     /translation="MVKLSVLDYALIDEGKDAQKALQDSVTLAKLADRLGFKRIWFTE
                     HHNVPAFACSSPELLMMHTLAQTNHIRVGSGGVMLPHYRPYKIAEHFRMMAALYPNRI
                     DLGIGNNPGTTMVKQALDGINPTYDSYDESISLLRDYLTIKDKPSAHTLGVQPHIDHF
                     PEMWLLSSSATSAKIAAELGIGLSVGTFLLPDINAIHAAKDNIDIYKKHFQASTIKMD
                     AKVMASVFVIVADNEAEVAALQHALDVWLLGKLQFAEFEHFPSVDTAQKYKLNDRDKE
                     MIQVHQARIIAGTQEQVKAQLDDFIATFEVDEVLVAPLIPGIEQRCKTLKLLAEIYL"
     gene            5841..6173
                     /locus_tag="PPY92_000006"
     CDS             5841..6173
                     /locus_tag="PPY92_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HDJ2624039.1"
                     /translation="MKKLANYLWVEKVGDLYVFSMTPELQDDIGTVGYVEFVSPDEVK
                     VDDEIVSIEASKTVIDVQTPLSGTIIERNTKAEEEPTILNSEKPEENWLFKLDDVDKE
                     AFLALPEA"
     gene            6174..6974
                     /locus_tag="PPY92_000007"
     CDS             6174..6974
                     /locus_tag="PPY92_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HDJ2624040.1"
                     /translation="METLKSNKARLEYLINDMRRERNDNDVLVMPSSFEDLWELYRGL
                     ANVRPALPVSDEYLAVQDAMLSDLNHQHVTDLKDLKPIKGDNIFVWQGDITTLKIDAI
                     VNAANSRFLGCMQANHDCIDNIIHTKAGVQVRLDCAEIIRQQGRNEGVGKAKKTRGYN
                     LPAKYIIHTVGPQIRRLPVSKMNQDLLAKCYLSCLKLADQHSLNHVAFCCISTGVFAF
                     PQDEAAEIAVRTVESYLKETNSTLKVVFNVFTDKDLQLYKEALNRDAE"
     gene            6964..7908
                     /locus_tag="PPY92_000008"
     CDS             6964..7908
                     /locus_tag="PPY92_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HDJ2624041.1"
                     /translation="MQSSKWNAMSLLMDEKTKQAEVLRTAIDEADAIVIGIGAGMSAS
                     DGFTYVGERFTENFPDFIEKYRFFDMLQASLHPYGSWQEYWAFESRFITLNYLDQPVG
                     QSYLALKSLVEGKQYHIITTNADNAFDAAEYDMTHVFHIQGEYILQQCSQHCHAQTYR
                     NDDLIRKMVVAQQDMLIPWEMIPRCPKCDAPMEVNKRKAEVGMVEDAEFHAQLQRYNA
                     FLEQHQDDKVLYLEIGIGYTTPQFVKHPFQRMTRKNENAIYMTMNKKAYRIPNSIQER
                     TIHLTEDISTLITTALRNDSTTQNNNIGETEDVLNRTD"
     gene            7886..8908
                     /locus_tag="PPY92_000009"
     CDS             7886..8908
                     /locus_tag="PPY92_000009"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDJ2624042.1"
                     /translation="MYLIEPIRNGEYITDGAIALAMQVYVNQHIFLDEDILFPYYCDP
                     KVEIGRFQNTAIEVNQDYIDKHSIQVVRRDTGGGAVYVDKGAVNMCCILEQDTSIYGD
                     FQRFYQPAIKALHTLGATDVIQSGRNDLTLNGKKVSGAAMTLMNNRIYGGYSLLLDVN
                     YEAMDKVLKPNRKKIASKGIKSVRARVGHLREALDEKYRDITIEEFKNLMVTQILGID
                     DIKEAKRYELTDADWEAIDELADKKYKNWDWNYGKSPKYEYNRSERLSSGTVDITISV
                     EQNRIADCRIYGDFFGQGDIKDVEEALQGTKMTREDLMHQLKQLDIVYYFGNVTVESL
                     VEMILS"
     gene            9224..10249
                     /locus_tag="PPY92_000010"
     CDS             9224..10249
                     /locus_tag="PPY92_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HDJ2624043.1"
                     /translation="MNNKVLVTGGTGFVGMRIISRLLEQGYEVQTTIRDLSKADKVIK
                     TMQDNGISTERLMFVEADLSQDEHWDEAMKDCKYVLSVASPVFFGKTDDAEVMAKPAI
                     EGIQRILRAAEHAGVKRVVMTANFGAVGFSNKDKNSITNESHWTNEDEPGLSVYEKSK
                     LLAEKAAWDFVENENTIVEFATINPVAIFGPSLDAHVSGSFHLLENLLNGSMKRVPQI
                     PLNVVDVRDVAELHILAMTNEQANGKRFIATADGQINLLEIAKLIKEKRPEIAQKVST
                     KKLPDFILSLGAKFNHQAKEGKLLLDMNRNVSNERAKTLLGWEPIATQEEAILAAIDS
                     MAKFHLI"
     gene            complement(10343..11689)
                     /locus_tag="PPY92_000011"
     CDS             complement(10343..11689)
                     /locus_tag="PPY92_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDJ2624044.1"
                     /translation="MQAILNFIVDILSQPAILVALIAFIGLIVQKKPAATITSGTIKT
                     ILGFLILSAGADVVVRSLEPFGKIFQHAFGVQGIVPNNEAIVSLALKDFGTTAALIMV
                     CGMIVNILIARFTNLKYIFLTGHHTFYMAAFLAIILTVSHIKGWLTIVIGALVLGLIM
                     AVLPALLQPTMRKITGNDQVALGHFGSISYFAAGAVGQLFKGKSKSTEEIKFPKGLSF
                     LRESTISISITMALLYFIACLFAGVSYVHESISDGQNFIVFSLIQGVTFAAGVFIILT
                     GVRLILAEIVPAFKGISEKLVPNSKPALDCPIVFPYAQNAVLIGFFVSFITGVIGMFI
                     LFLFGGVVILPGVVAHFFLGATAAVFGNARGGIKGAVAGAALNGILITFLPLLFLPFL
                     GELGGAATTFSDTDFLTVGIVFGNAVKYMGLFGAILFIIIVGATAILLKGRQKPQQ"
     gene            complement(11704..11988)
                     /locus_tag="PPY92_000012"
     CDS             complement(11704..11988)
                     /locus_tag="PPY92_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDJ2624045.1"
                     /translation="MKILVVCGHGLGSSFMVEMNAQEALRQLNAPSDIEVEHSDIMTA
                     SPEMADLFICGRDLAENAERLGDVLVLDNILDKAELQQKLSEKLQQLNMI"
     gene            complement(11990..12433)
                     /locus_tag="PPY92_000013"
     CDS             complement(11990..12433)
                     /locus_tag="PPY92_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDJ2624046.1"
                     /translation="MSLDILSTTRIIVKEQVNDWTEAITIASQPLLQEQIIEQGYVQA
                     MIDSVNELGPYIVIAPEIAIAHARPNNDVHQVGLSLLKLNQHVAFCDEDHYASLIFVL
                     SAIDNHSHLSVLQNLATVLGDNQTVQQLLTAKNAQDIKNILKEHD"
     gene            complement(12438..14393)
                     /locus_tag="PPY92_000014"
     CDS             complement(12438..14393)
                     /locus_tag="PPY92_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDJ2624047.1"
                     /translation="MLSYRQIEILYLLIHEQTFIPINTIANQLGVSPRTIQYDIAYIE
                     QYAETYHYQVSRNKAAGIKVTTAHATLLNELEHNLTNQIHFSKDERLTHIALKLFETT
                     DPVSTKQLAQDVNVSRRTIADDIKMIQAQLDQYHLKLNYVHNKGFNVIGEEDHYRKAY
                     AHFIHQYMKQAAPFIEADIFNSESIALVRRAIIKTLNSENYHLVQSAIDGLIYHILIA
                     IQRLNENFSFDIPINEIDKWRHTNQYAIASKMIENLERSCNVTFPESEIIFITLHLLG
                     SKMTEHTASSITFEYHDLSQNIHELITCVSQELGIDMSKDNKLHTSLITHIKPAIHRI
                     KYDMLQPNPLRQEVMRRYPQIIEAVSKHISPIEQDAAIRFNEDELTYITIHFASSIER
                     VATHKQSMIKVVLLCGSGIGTSQLLKSKLNHLYPEFHIWDAYSIYQLEESRLLQDNID
                     YVISTVPCEISAVPVIHVDPFINQQSRQKLNQIINDSREQRVMKMATDGKSLADLLPE
                     HRIIINEQPLSIESAIAVAVQPLINDGIVYSNYTAAILKQFEQFGSYMVISPHIALIH
                     AGTDYVQNGVGFALTYFTEGIIFGSKANDPVHLVITLATDHPNAHLKALGQLSECLSN
                     DLYRQDFLDGNIFKIKQHIALTMTKEV"
     gene            14605..15024
                     /gene="mepR"
                     /locus_tag="PPY92_000015"
     CDS             14605..15024
                     /gene="mepR"
                     /locus_tag="PPY92_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor MepR"
                     /protein_id="HDJ2624048.1"
                     /translation="MEFTYSYLFRMISHEMKQKADQKLEQFDITNEQGHTLGYLYAHQ
                     QDGLTQNDIAKALQRTGPTVSNLLRNLERKKLIYRYVDAQDTRRKNIGLTTSGIKLVE
                     AFTSIFDEMEQTLVSQLSEEENEQMKANLTKMLSSLQ"
     gene            15131..16486
                     /gene="mepA"
                     /locus_tag="PPY92_000016"
     CDS             15131..16486
                     /gene="mepA"
                     /locus_tag="PPY92_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498905.1"
                     /note="MepA (multidrug export protein A), as described
                     originally in Staphylococcus aureus, is a MATE transporter
                     shown to be over-expressed after culturing in the presence
                     of tigecycline, owing to mutation of its repressor MepR,
                     and then to be one of several factors contributing to
                     elevated tigecycline resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MepA"
                     /protein_id="HDJ2624049.1"
                     /translation="MKDEQLYYFEKSPVFKAMMHFSLPMMIGTLLSVIYGILNIYFIG
                     FLEDSHMISAISLTLPVFAILMGLGNLFGVGAGTYISRLLGAKDYSKSKFVSSFSIYG
                     GIALGLIVILVTLPFSDQIAAILGARGETLALTSNYLKVMFLSAPFVILFFILEQFAR
                     AIGAPMISMIGMLASVGLNIILDPILIFGFDLNVVGAALGTAISNVAAALFFIIYFMK
                     NSDVVSVNIKLAKPNKEMLSEIFKIGIPAFLMSILMGFTGLVLNLFLAHYGNFAIASY
                     GISFRLVQFPELIIMGLCEGVVPLIAYNFMANKGRMKDVIKAVIMSIGVIFVVCMIAV
                     FTIGHHMVGLFTTDQAIVEMATFILKVTMASLLLNGIGFLFTGMLQATGQGRGATIMA
                     ILQGAIIIPVLFIMNALFGLTGVIWSLLIAESLCALAAMLIVYLLRDRLTVDTSELIE
                     G"
     gene            16590..17048
                     /locus_tag="PPY92_000017"
     CDS             16590..17048
                     /locus_tag="PPY92_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HDJ2624050.1"
                     /translation="MYKSKILLKYIFSEESEVKDLTEEKYNQDYEALTFSFKEETYQS
                     RLAKKTPTKAGYFVTCWTKDENNCNQPYSKEAFADYLMIIVIDEELSGYFLFPRELLV
                     EKGILTTFEHKGKMAFRVYPKWCNQLNKTAGQTQKWQCKYFLNTNKKSYG"
     gene            complement(17112..18470)
                     /gene="glpT"
                     /locus_tag="PPY92_000018"
     CDS             complement(17112..18470)
                     /gene="glpT"
                     /locus_tag="PPY92_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDJ2624051.1"
                     /translation="MNFLKPAKHIKPLPENQIDDTYKRLRLQVFLGIFIGYAGYYLLR
                     KNFSLAMPALQEQGFTKAELGFALSAVSIAYGFSKFFMGTVSDRSNARIFLVLGLALT
                     AIVNLLMGFVPFFTSGIGIMFVLLFLNGWFQGMGWPPSGRVLVHWFSVSERGSKTALW
                     NVAHNVGGGIMAPIAAWGITTTAFINFGYLKGFEGVFIYPALLALIIAAISYVLIRDT
                     PQSQGLPPIEIYKNDFATSDKKTLETELTTKEILFKYVLNNKWVWAIAFANIFVYFVR
                     YGVLDWAPVYLSEEKHFDLKASGWAYFLYEWAGIPGTLLCGYISDKLFKGRRGPAGFF
                     FMLGVTVFVLIYWLNPPGNAWLDNVSLIAIGFLIYGPVMLIGLQALDYVPKKAAGTAA
                     GLTGLFGYLFGAVMANIVLGAVVDKFGWDVGFILLTAISVFAMLSFILTWNKVGQETV
                     HH"
     gene            18782..19708
                     /locus_tag="PPY92_000019"
     CDS             18782..19708
                     /locus_tag="PPY92_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDJ2624052.1"
                     /translation="MNIVGHHHISMYTKDAKRNKDFYTNVLGLRLVEKSVNQDNPSMY
                     HLFYGDEVGTAGTILSFFEIPHAGHKQPGTETIYRFSLLVPNQAALHYFEKRLDNNGI
                     TSERLYYLGQEGVVFKDEDDLEIILLVNDSFEVPHQWQHNAYSEIPQVYQILGIGPVE
                     LRVRNAARTVEFLENVLGYRKRDNKSFDVLTLAPQGLYSDFVVIEQQGQRERPGRGYI
                     HHIAINTPQMSDLDAIYKKLQQQTQSNSGIIDRYFFKSLYYRHNSIMYEFATEAPGFT
                     IDTPLEQLGSQLNLPDFLEAEREQIESKLHEI"
     gene            19722..20783
                     /locus_tag="PPY92_000020"
     CDS             19722..20783
                     /locus_tag="PPY92_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDJ2624053.1"
                     /translation="MAKLEMNKNTPLEFGLYSLGDHLLNPFKGEKVSYEQRINEIIEA
                     SKLADETGIDVFAVGESHQEHFTTQAHTVVLGAIAQATKHIKVSSSSTIISATDPVRV
                     FEDFATLDLISHGRAEIVAGRASRTGIFDLFGYDLKDYDELFEEKLGLLLELNKTERI
                     TWSGKYRPELRNMKIFPRPIDNILPIWRAVGGPPASAIKAGKQGVPMMITTLGGPAMN
                     FKGSIDAYRQAATEAGFDASHKSLPVSTASLFYTAETTQDAMREFYPHLNTGMSFIRG
                     VGYPKQQFANSSDYREALMVGSPQQIIEKILYQHELYGHQRFMAQLDFGGVPFENVMK
                     NIELIGNEIIPAIKKHLSK"
     gene            20797..21363
                     /locus_tag="PPY92_000021"
     CDS             20797..21363
                     /locus_tag="PPY92_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDJ2624054.1"
                     /translation="MNIVLLSGSTVGSKTRIAMDDLKNELEVINEGHQIELMDLRELE
                     LEFSVGKNYLDTTGDVYKLTTSLMQADVIFIGFPIFQASIPGALKNVFDLLPVNAFRD
                     KVIGLVATAGSSKHYLIPEMHLKPILSYMKAHTMQTYVFIEEKDFSNQQIVNDDVVFR
                     LKALAQFTMRTAKVQQQVFEEENNQYDF"
     gene            complement(21422..22417)
                     /locus_tag="PPY92_000022"
     CDS             complement(21422..22417)
                     /locus_tag="PPY92_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HDJ2624055.1"
                     /translation="MASLKNKHFMIGLSLTFIVALFSFLAAKLPILDKVGALTIAILI
                     AILYRHFRGYPEQYSSGITFSSKYLLRFAIILYGLKLNIFDIIGQGSKLLAIDVGVVI
                     FSIVMMLFVNKLLHGDKNIALLLGVGTGVCGAAAIAAVAPIFKSREKDTAISIGIIAL
                     IGTIFSLIYTAIYAIFSMTTNVYGAWSGVSLHEIAHVVLAGGFGGSDALKIALLGKLG
                     RVFLLIPLTIVLILIMRFRSSESSSKGRISIPYFLIGFVIMALVNTYVTIPSVLLNIL
                     NTISTICLLMAMVALGLNVAFKDLKNRALKPLMTIIITSICLSSLAFIVVHWLYS"
     gene            22782..23327
                     /locus_tag="PPY92_000023"
     CDS             22782..23327
                     /locus_tag="PPY92_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDJ2624056.1"
                     /translation="MFGMKVNEQITLKILEAHDTEALFNLVNRSRNSLREWLPWVDAT
                     EQPSDTRAFIKRGLLQFADGNGFQCGIWYEGTLVGVIGLHEINHMHRKTSLGYYLDKQ
                     FEGHGIMTQAVEALIKYCFEELDLNRIEISAAVNNEKSRAIPERLGFTREGMLRDNEL
                     LNGIYSSSYIYSLLKSEYDQK"
     gene            23594..24448
                     /locus_tag="PPY92_000024"
     CDS             23594..24448
                     /locus_tag="PPY92_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624057.1"
                     /translation="MKKLTTLLLASTLLIAACGNDDSKKDDSKTSKKDDGVKAELKQA
                     TKAYDKYTDEQLNEFLKGTEKFVKAIENNDMAQAKALYPKVRMYYERSEPVAEAFGDL
                     DPKIDARLADMKEEKKEKEWSGYHKIEKALYEDKKIDDVTKKDAQQLLKDAKELHAKA
                     DTLDITPKLMLQGSVDLLNEVATSKITGEEEIYSHTDLYDFKANVEGAQKIYDLFKPI
                     LEKKDKKLSDDIQMNFDKVNQLLDKYKDNNGGYESFEKVSKKDRKAFADAVNALGEPL
                     SKMAVITE"
     gene            24445..25674
                     /gene="efeB"
                     /locus_tag="PPY92_000025"
     CDS             24445..25674
                     /gene="efeB"
                     /locus_tag="PPY92_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HDJ2624058.1"
                     /translation="MTNYEQVNDSTQFSRRTFLKMLGIGGAGVAIGASGVGSMWSFKS
                     MFNTPEDPEKDAYEFYGKVQPGITTPTQKTCNFVALDLKSKDRDAIKAMFKKWTVMAD
                     RMMDGDTVGKTSNNPLMPPVDTGESIGLGASKLTITFGISKSLMKKIGLSSKIPDAFK
                     DLPHFPNDQLIDDYSDGDIMIQACSNDSQVSFHAVHNLVRPFRDIVKVRWAQSGFISA
                     KGKETPRNLMAFKDGTINPRKSNQLKDYVFIDDGWAKHGTYCVVRRIQIHIETWDRTA
                     LEEQEATFGRKRHSGAPLTGGKEFDEIDLKAKDSHGEYIIDKDAHTRLAKEANTSILR
                     RAFNYVDGTDDRTGNFETGLLFIAFQKATKQFIDIQNNLGSNDKLNEYITHRGSASFL
                     VLPGVSKGGYLGETLFD"
     gene            25655..27367
                     /locus_tag="PPY92_000026"
     CDS             25655..27367
                     /locus_tag="PPY92_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HDJ2624059.1"
                     /translation="MKHYLTKFVAMLITAAMVCSFGLLKSQAAEQQSISDVYSVITDA
                     KSALSNNSISNDNKQKAIEQVVSAVKKLSLEDNSEGNAVKSDVRKLEDAKANDNQKDT
                     LSQLTKSLIAYEEKLASKDAGSKIKLLQQQVDAKDAAMTKAIKDKNKAELESLNNSLN
                     QIWTSNETVIRNYDANQYGQIEVALLQLRIAIHKSPLDTAKVSHAWTTFKSNIDHVDK
                     KSDTSANDQYHVSQLNDALEKAIKAIDDNQLSDADAALTHFIEIWPYVEGQIQTKDGA
                     LYTKIEDKIPYYQSVLDEHNKSHVKDGLVDINNQIKEVVGHSYSFVDVMIIFLREGLE
                     VLLIVMTLTTMTRKVKDNKGTASVIGAAIAGLVLSIILAITFVETLGNSGILRESMEA
                     GLGIVAVILMFIVGVWMHKRSNAKRWNDMIKNMYANAISNGNLVLLATIGLISVLREG
                     VEVIIFYMGMIGELATKDFIIGIALAIVILIVFAVLFRFIVRLIPIFYIFRVLSIFIF
                     IMGFKMLGVSIQKLQLLGAMPRHVIEGFPTINWLGFYPSYEPLIAQAAYIMVVAILIF
                     KFKK"
     gene            complement(27608..28270)
                     /gene="tatC"
                     /locus_tag="PPY92_000027"
     CDS             complement(27608..28270)
                     /gene="tatC"
                     /locus_tag="PPY92_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HDJ2624060.1"
                     /translation="MLLSFVVTVIVVYVSSFWWMTPFITYITRAHVSLHAFSFTEMIQ
                     IYVMIIFFIAFCFISPVMFYQLWAFIAPGLHNNERQFIYKYSFFSVLLFCAGVAFAFY
                     VGFPIIIQFALKLSLTLNISPVIGFKAYLVELIRWLFTFGILFQLPILFIGLAKFGLI
                     DITSLKHYRKYIYFACFVLASIIAPPDLTLNILLTLPLILLFEFSMFIVKFTCRGKPP
                     TH"
     gene            complement(28326..28550)
                     /locus_tag="PPY92_000028"
     CDS             complement(28326..28550)
                     /locus_tag="PPY92_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HDJ2624061.1"
                     /translation="MIIMITNTFILGITGPTSLVVISIIALIIFGPKKLPQFGSAIGS
                     TLKEFKSATEDLDKESHDTPSKESKQQREQ"
     gene            complement(28649..29038)
                     /locus_tag="PPY92_000029"
     CDS             complement(28649..29038)
                     /locus_tag="PPY92_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HDJ2624062.1"
                     /translation="MAFTLSAIQQAHQQFTGVDFPKLFKAFKDMGMTYNIVNIQDGTA
                     TYVHQSEDDIVTSSVKSNHPVAQKSNKTIVQDVLTRHQQGQTDFETFCDEMAEAGIYK
                     WHIDIQAGTCTYIDLKEQAIISELIPQ"
     gene            29278..29481
                     /locus_tag="PPY92_000030"
     CDS             29278..29481
                     /locus_tag="PPY92_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDJ2624063.1"
                     /translation="MRNRLKELRARDGLNQTQLAKLAGVSRQTISLIERNNFMPSVLT
                     AIKIARIFNETVETVFIIEEDEA"
     gene            29478..30191
                     /locus_tag="PPY92_000031"
     CDS             29478..30191
                     /locus_tag="PPY92_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HDJ2624064.1"
                     /translation="MKILRYIGYLLLGGIVGGIIGGILGNFDGLGIENLTFATYNNVV
                     VISIVATMIIILVEAIVLMNQRRALKYKRLVDEEVDIDATDQYELLANRYVLNGSILS
                     VIQTIIAFVVLLIFVVGQAEANAMLFFLIPFFASAIFNTQFTLFNRKFDDRMPKIADK
                     NYTEKRLEILDEGERHIELIALFKTYAINLSILILAIIFIGSYSIATGINQSFSLLLI
                     IAIFIYNAFSYLLKRRRFY"
     gene            30216..31058
                     /locus_tag="PPY92_000032"
     CDS             30216..31058
                     /locus_tag="PPY92_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDJ2624065.1"
                     /translation="MTTLLNVDRVNKQYKDSDFKLQDASLTISTNETVGLIGKNGSGK
                     STLINILVGNRHKDNGSIAFFGEEHAADDVEYKEHIGVVFDDLRVPNKLTIKDIDKVF
                     QSIYTTWNSQKFFDLIKYFELPLQTKIKTFSRGMRMKIALTIALSHDVKLLILDEATA
                     GMDVSGREEVMELLEDFVAQGGGILISSHISEDIEQLADKLVFMKDGRMILTEQKDIL
                     LAQYGIVTTGDKDVEIPKHLIIASRLSKGKYQILVKDYAEIENAEPLKHIDDATKIIM
                     RGEV"
     gene            31058..31687
                     /locus_tag="PPY92_000033"
     CDS             31058..31687
                     /locus_tag="PPY92_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDJ2624066.1"
                     /translation="MKGMFLSSFYATRKQTYIYFIVAIIAAGYFAVFNPLMSSAMAGV
                     MLITPITDNIKHEKDSRWMYYVSTLPVKRSDYIKSYFAFYLILFGASLMIGLVVTTIV
                     TQSVMIGIMSGLMSFGIIGAYSIIFPLTFKFGAENSNVIMIVASILLLFSFVVFFFIY
                     GMVSGASALEFEKISTEGWLVVIVYAVIGIVITSVSYILSIKIFNKQEL"
     gene            complement(32074..33207)
                     /locus_tag="PPY92_000034"
     CDS             complement(32074..33207)
                     /locus_tag="PPY92_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HDJ2624067.1"
                     /translation="MVNALTPKHLAEKRAGFHELFFDLIFVYAIQKIAHVILTTQNGS
                     ISADLFFKYIVMSLFLWLMWSHQTFFTNRFGQVTFKDVSFMMFNMFIMVFLSNSLYPD
                     FEKTFFPFFLCVAIMYLSIGLQYLLHIRTGLDYGDKRTCQAFASVAFVISFLSFLSLV
                     LPQSIHYIPGFLGVFIAATGLIPFQKYLVLSPVNMMHLVERFSLLTIIIFGEVLVGLA
                     SSSFSIDHFSYIYIFQFMILISLFGVYWIITENYINHKLSSIGFRLSYTHLLINIALG
                     VINAAIVFSNNNKLNDLFEINMMYISVLIFYIGLWLITPYFHNELTNAKYISSSLGIL
                     VVSYIISLIFKGHDQVMIISVSVATFCIMLIYFKNQRLRNKHE"
     gene            33746..34927
                     /locus_tag="PPY92_000035"
     CDS             33746..34927
                     /locus_tag="PPY92_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDJ2624068.1"
                     /translation="MTRVVLAAAYRTPIGVFGGAFKDVPAYDLGATLIEHIIKETGLN
                     PSEIDEVIIGNVLQAGQGQNPARIAAMKGGLPETVPAFTVNKVCGSGLKSIQLAYQSI
                     VTGENDIVLAGGMENMSQSPMLVNNSRFGFKMGHQSMVDSMVYDGLTDVFNQYHMGIT
                     AENLVEQYGISREEQDTFAVNSQHKAVRAQQNGEFDSEIVPVSIPQRKGEPILVTKDE
                     GVRENVSVEKLSRLRPAFKKDGTVTAGNASGINDGAAMMLVMSEDKAKELNIEPLAVL
                     DGFGSHGVDPSIMGIAPVGAVEKALKRSKKELSDIDVFELNEAFAAQLLAVDRELKLP
                     PEKVNVKGGAIALGHPIGASGARVLVTLLHQLNDEVETGLTSLCIGGGQAIAAVVSKY
                     K"
     gene            complement(35010..35762)
                     /locus_tag="PPY92_000036"
     CDS             complement(35010..35762)
                     /locus_tag="PPY92_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HDJ2624069.1"
                     /translation="MTQYPLWQQLNTLKQATWVDLTHTFDPEIPRFSEFEKGEVSTLF
                     TVKDHGFYVQRWNIVTQYGTHIDAPIHFVENKRYLEDIDLKELVLPLIVLDFSTEVAN
                     NNDFIVTRAHIEAWEKEHGTIEPGTFVALRTDWSKRWPNIEKFENKDANGQQHAPGWG
                     LDALKYLIEERRVEAVGHETFDTDASVDVVKNGDLVGERYILGQDKYQVELLTNLDQL
                     PTRGAIIYAISPKPKDAPGFPVRAFAIKPSND"
     gene            complement(35805..38033)
                     /gene="metE"
                     /locus_tag="PPY92_000037"
     CDS             complement(35805..38033)
                     /gene="metE"
                     /locus_tag="PPY92_000037"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDJ2624070.1"
                     /translation="MTTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDL
                     HKENLLLQKYYHLDSIPVGDFSLYDHILDTSLLFNIIPERFQGRTIDDDLLFDIARGN
                     KDHVASALIKWFNTNYHYIVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPIT
                     FVKLSKGGHQTFEEKVKTLLPLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITR
                     EAYDYFEKAGVAKKLVIQTYFERAHLKFLSSLPVGGLGLDFVHDNGYNLKQIEAGDFD
                     KSKTLYAGIIDGRNVWASDIEAKKVLIDKLLAHTNELVIQPSSSLLHVPVSLDDETLD
                     TSVGEGLSFATEKLDELDALRRLLNQNDSVKYDKLKARYERFQNQSFKNLDYDFESVR
                     TSRQSPFAQRIEQQQKRLNLPDLPTTTIGSFPQSREVRKYRADWKNKRITDEAYETFL
                     KNEIARWIKIQEDIGLDVLVHGEFERNDMVEFFGEKLQGFLVTKFGWVQSYGSRAVKP
                     PIIYGDVKWTAPLTVDETVYAQSLTDKPVKGMLTGPVTILNWSFERVDLPRKIVQDQI
                     ALAINEEVLALEAAGIKVIQVDEPALREGLPLRSEYHEQYLKDAVLSFKLATSSVRDE
                     TQIHTHMCYSQFGQIIHAIHDLDADVISIETSRSHGDLIKDFEDINYDLGIGLGVYDI
                     HSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTRKEEEVKDALTVLVNAVKAKR
                     QE"
     gene            complement(38030..39871)
                     /locus_tag="PPY92_000038"
     CDS             complement(38030..39871)
                     /locus_tag="PPY92_000038"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HDJ2624071.1"
                     /translation="MSQFLTQLKDNVLVADGAIGTILYSEGLDTCPEAYNLSHPDKVE
                     RIHRSYIEAGADVIQTNTYGANFEKLKRFGLEDKVKAIHQAAVRIAKKAANKDTYILG
                     TVGGFRGIKQEDISLQTILYHTEIQIDTLIEEGVDALLFETYYDLEELTNVISRTRKK
                     YDIPIIAQLTASNTNYLVNGQAINEGLKQLVQCGANIVGLNCHHGPHHMQESFTHIEL
                     PEHAFLSCYPNASLLDIENSEFKYSDNAQYFGQVAQNLIREGVRLIGGCCGTTPEHIK
                     FIKESIQTLKPVNDKKVIPIPTKALFNPSQNKVRQSLTSKVQERPTVIIELDTPKHLD
                     TDRFFENIAKLDKANVDAVTLADNSLATVRISNIAAASLIKQYYNIEPLVHITCRDRN
                     LIGLQSHLLGLSLIGVNEILAITGDPSKVGHLPGATNVYDVNSKGLTELALRFNQGIN
                     TDGDALKKRTHFNIAGAFNPNVRKLDGAVKRLEKKIESGMSYFITQPVYSKEKIIEIY
                     HATKHLNKPFFIGIMPIASYKNALFLHNEVPGIKMSDEILQQFEAVKDDKAKTRELSL
                     KLSKDLIDTVHEYFNGLYIITPFQNVEDSLELAAYSKSITAHKEAIL"
     gene            complement(39840..41000)
                     /locus_tag="PPY92_000039"
     CDS             complement(39840..41000)
                     /locus_tag="PPY92_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HDJ2624072.1"
                     /translation="MTLSKETEVIFDWRRGVEYHSANPPLYDSSTFHQTSLGGDVKYD
                     YARSGNPNRELLEEKLARLEQGKFAFAFASGIAAISAVLLTFKSGDHVILPDDVYGGT
                     FRLTEQILNRFNIEFTTVDTTKLEQIEGAIQSNTKLIYIETPSNPCFKITDIKAVSKI
                     AEKHELLVAVDNTFMTPLGQSPLLLGADIVIHSATKFLSGHSDLIAGAVITNNEAISE
                     ALYLIQNGTGNMLSAQDSWTLAKHLKTFPIRFKQSVENAQKIVSFLIKQDEISEVYYP
                     GLTTAHLEQAKNGGAVIGFRLADESKAQQFVDALTLPLVSVSLGGVETILSHPATMSH
                     AALPEEVRQERGITFGLFRLSVGLEDPDELIADIKYALKEAFNESIPHTIER"
     gene            complement(40997..42100)
                     /locus_tag="PPY92_000040"
     CDS             complement(40997..42100)
                     /locus_tag="PPY92_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDJ2624073.1"
                     /translation="MKDTQLAQITLTDDSTGAIANPIHLSTAYKHPKLGQSTGFDYTR
                     TKNPTRSTFETCFAKLEHGIASFATSSGMSAIQLICNLFKPHDEILVSFDLYGGTFRL
                     FEFYEQQYDIKFKYVDFTDYEQVEKEITDKTVALFIEPISNPQMIAIDVKPYYQLCKA
                     KGLLSIIDNTFLTPYLSTPLAEGADIVLHSATKYIGGHNDVLAGVVTVKDESLAQQLF
                     DFHNMTGATLSPIDSYLLLRGLKTLHLRIERAQSNARKLAKKCQSLQAIDEVLYSGQT
                     GMLSLRLNKAYSVAKLLENLDICIFAESLGGTETLVTFPYTQTHVDMPDAEKDKRGID
                     EYLIRLSLGVENYEDIERDIIQALDKAQIGEIV"
     misc_binding    complement(42164..42533)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            42762..43607
                     /locus_tag="PPY92_000041"
     CDS             42762..43607
                     /locus_tag="PPY92_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDJ2624074.1"
                     /translation="MSELSKSEDQRITKTKDEQIKQIDISDIKPNPYQPRKTFDENHL
                     NDLADSIKQYGILQPIVLRKTVQGYYIVVGERRFRASKIAGLKYVSAIIKDLTDEDMM
                     ELAVIENLQREDLNAIEEAESYQRLMTDLKITQQEVAKRLSKSRPYIANMLRLLHLPK
                     KIADMVKDGRLTSAHGRTLLAIKDEQQMLRLAKRVVKEKWSVRYLENHVNELKNVSSK
                     SKTDKVDITKPKFIKQQERQLREQYGTKVDISIKKSVGKISFEFDSQDDFVRIIEQLN
                     RRYGK"
     gene            43761..44642
                     /locus_tag="PPY92_000042"
     CDS             43761..44642
                     /locus_tag="PPY92_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HDJ2624075.1"
                     /translation="MDQVMSIISSLFEPLTKIETYENIATKIAMIVIYIIVALIVIKI
                     LNKMIEQGFKIQNKSKKSNKKRSKTLISLVQNVVKYIVWFIVITTILSKFGISVEGVI
                     ASAGVVGLAVGFGAQTIVKDVITGFFIIFESQFDVGDYVKINNGGTTVAEGTVKSIGL
                     RSTRINTISGELTILPNSSMGEITNYSITNGTAIVKIPVSVDENIDNVEKKLNKLFTS
                     LRSKYYLFVSDPVVIGIDAFEDTRVILRVSAETIPGEGFSGARIIRKEVQKMFLQEGI
                     KTPQPIMTPFNHSENGV"
     gene            44672..44875
                     /locus_tag="PPY92_000043"
     CDS             44672..44875
                     /locus_tag="PPY92_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDJ2624076.1"
                     /translation="MASKYGINDIVEMKKQHACGTNRFKIIRMGADIRIKCENCQRSI
                     MIPRQTFDKKLKKIIVSQDDTQR"
     gene            44887..45984
                     /gene="ychF"
                     /locus_tag="PPY92_000044"
     CDS             44887..45984
                     /gene="ychF"
                     /locus_tag="PPY92_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDJ2624077.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGALAANYPFATIDPNVGIV
                     EVPDARLLKLEEMVQPKKTLPTTFEFTDIAGIVKGASKGEGLGNKFLSHIREVDAICQ
                     VVRAFDDDNVTHVAGRVDPIDDIEVINMELVLADLESVEKRLPRIEKLARQKDKTAEM
                     EVRILTTIKEALENGKPARSIDFNEEDQKWVNQAQLLTSKKMLYIANVGEDEIGDDDN
                     DKVKAIREYAAQEDSEVIVISAKIEEEIATLDDEDKEMFLEDLGIEEPGLDRLIRTTY
                     ELLGLSTYFTAGVQEVRAWTFKQGMTAPQCAGIIHTDFERGFIRAEVTSYDDYVQYGG
                     ESGAKEAGRQRLEGKEYIMQDGDIVHFRFNV"
     gene            complement(46070..46261)
                     /locus_tag="PPY92_000045"
     CDS             complement(46070..46261)
                     /locus_tag="PPY92_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624078.1"
                     /translation="MNLNIDWSKDFQEFQEILNSGIHPEWLYCAKANLVLEPAYTGEG
                     KQFFSTQDIIYASKIIPFF"
     gene            46505..46801
                     /locus_tag="PPY92_000046"
     CDS             46505..46801
                     /locus_tag="PPY92_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDJ2624079.1"
                     /translation="MRTYEVMYIVRPNIEEDAKKALVERFNGILATEGAEVLEAKDWG
                     KRRLAYEINDFKDGFYNIVRVKSDNNKATDEFQRLAKISDDIIRYMVIREDEDK"
     gene            46822..47325
                     /gene="ssb"
                     /locus_tag="PPY92_000047"
     CDS             46822..47325
                     /gene="ssb"
                     /locus_tag="PPY92_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDJ2624080.1"
                     /translation="MLNRVVLVGRLTKDPEYRTTPSGVSVATFTLAVNRTFTNAQGER
                     EADFINCVVFRRQADNVNNYLSKGSLAGVDGRLQSRNYENQEGRRVFVTEVVCDSVQF
                     LEPKNAQQNGGQRQQNEFQDYGQGFGGQQSGQNNSYNNSSNTKQSDNPFANANGPIDI
                     SDDDLPF"
     gene            47377..47619
                     /gene="rpsR"
                     /locus_tag="PPY92_000048"
     CDS             47377..47619
                     /gene="rpsR"
                     /locus_tag="PPY92_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002484306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDJ2624081.1"
                     /translation="MAGGPRRGGRRRKKVCYFTANGITHIDYKDTELLKRFISERGKI
                     LPRRVTGTSAKYQRMLTTAIKRSRHMALLPYVKEEQ"
     gene            complement(47896..48834)
                     /locus_tag="PPY92_000049"
     CDS             complement(47896..48834)
                     /locus_tag="PPY92_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDJ2624082.1"
                     /translation="MLNFDEQIAKLKQMNIFFNIIDTEKANEILRKNNYFFKLAYFRK
                     NFEKKNGGYFIEFAYLSDLATIDMKLRYTMLHLTLDIEHSLKCLVLKLITENNQEDGY
                     KIIDEFLCIDKSYSNSNFDTNSRTPEEVMETKIKNKNEIFKHMNKRGQLPEKLNKYYQ
                     NPPAWVCIEFMQLGQFVSFLNFYYKKYNDEELRVANILMPLVKNIRNKSAHNQPIIAN
                     LNYDSRSPQYLFEKGNNIGISRNMFGIKNFIDTFATLELHNQVCSNAIIQARYHDLDQ
                     LQKRYKRNESYYNNALAIKRFFIALDKIIDFNRPKV"
     gene            complement(48873..49586)
                     /locus_tag="PPY92_000050"
                     /pseudo
     CDS             complement(48873..49586)
                     /locus_tag="PPY92_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270135.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            49792..50403
                     /locus_tag="PPY92_000051"
     CDS             49792..50403
                     /locus_tag="PPY92_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDJ2624083.1"
                     /translation="MFKKYDSKNSIVLKSILSLGIIYGGTFGIYPKADASTQNSSSVQ
                     DKQLQKVEEVPNNSEKALVKKLYDRYSKDTINGKSNKSRNWVYSERPLNENQVRIHLE
                     GTYTVADRVYTPKRNITLNKEVVTLKELDHIIRFAHISYGLYMGEHLPKGNIVINTKD
                     GGKYTLESHKELQKDRENVKINTADIKNVTFKLVKSVNDIEQV"
     gene            50772..51140
                     /locus_tag="PPY92_000052"
     CDS             50772..51140
                     /locus_tag="PPY92_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDJ2624084.1"
                     /translation="MKTILKTITYLALTIIGAYAALFILKTIDSHGITDQFNPLVKED
                     DSYVKTTEVSTRMDDQLRSYTQSAFNKEGKETQLMYTATFDVKPHRYLKITHKGHHVE
                     TFEEVEKEEVPKKALDKLSR"
     gene            51321..51893
                     /locus_tag="PPY92_000053"
     CDS             51321..51893
                     /locus_tag="PPY92_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDJ2624085.1"
                     /translation="MKLKSLAVLSMSAVVLTACGNDTPKDETKSTESNTNQDTNTTKD
                     VIALKDVKTSPEDAVKKAEETYKGQKLKGISFENSNGEWAYKVTQQKSGEESEVLVAD
                     KNKKVINKKTEKEDTVNENDNFKYSDAIDYKKAIKEGQKEFDGDIKEWSLEKDDGKLV
                     YNIDLKKGNKKQEVTVDAKNGKVLKSEQDH"
     gene            complement(52030..52293)
                     /locus_tag="PPY92_000054"
     CDS             complement(52030..52293)
                     /locus_tag="PPY92_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDJ2624086.1"
                     /translation="MNMHILYNLRTKHNLEIDELAQQLNEKYGTKYEAHQIWEWENHH
                     HEPKFKDAMHLADFFDAPYEMFLESKVKEYQKHLEEVDIRMDK"
     gene            52593..52844
                     /locus_tag="PPY92_000055"
     CDS             52593..52844
                     /locus_tag="PPY92_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDJ2624087.1"
                     /translation="MFGFIGMLIVGGLIGWAAGAIMGKDIPGGILGNIIAGIIGSWVG
                     GKLFGQWGPELGSIYILPALIGSIILIAIVTLILRAMRK"
BASE COUNT        18032 a         7987 c        10110 g        16820 t
ORIGIN      
        1 aaaaataata tatgtcatgt tatattgaag gtgcagttgt ttttcattct caagaggggg
       61 tcaaaaaaat acttttgagg tgattatatg ttaagaggac aagaagaaag aaagtatagt
      121 attagaaagt attcaatagg cgtggtgtca gtgttagcgg ctacaatgtt tgttgtgtca
      181 tcacatgaag cacaagcctc ggaaaaaaca ccaacgtcca atgcagcggc acaaaaagaa
      241 acactaaatc aaccgggaga acaagggaac gcgataacgt cacatcaaat gcagtcagga
      301 aagcaattag acgatatgca taaagagaat ggtaaaagtg gaacagtgac agaaggtaaa
      361 gacacgcttc aatcatcgaa gcatcaatca acacaaaata gtaaaacaat cagaacgcaa
      421 aatgataatc aagtaaagca agattctgaa cgacaaggtt ctaaacagtc acaccaaaat
      481 aatgcgacta ataatactga acgtcaaaat gatcaggttc aaaataccca tcatgctgaa
      541 cgtaatggat cacaatcgac aacgtcacaa tcgaatgatg ttgataaatc acaaccatcc
      601 attccggcac aaaaggtatt acccaatcat gataaagcag caccaacttc aactacaccc
      661 ccgtctaatg ataaaactgc acctaaatca acaaaagcac aagatgcaac cacggacaaa
      721 catccaaatc aacaagatac acatcaaccc gcgcatcaaa tcatagatgc aaagcaagat
      781 gatactgttc gccaaagtga acagaaacca caagttggcg atttaagtaa acatatcgat
      841 ggtcaaaatt ccccagagaa accgacagat aaaaatactg ataataaaca actaatcaaa
      901 gatgcgcttc aagcgcctaa aacacgttcg actacaaatg cagcagcaga tgctaaaaag
      961 gttcgaccac ttaaagcgaa tcaagtacaa ccacttaaca aatatccagt tgtttttgta
     1021 catggatttt taggattagt aggcgataat gcacctgctt tatatccaaa ttattggggt
     1081 ggaaataaat ttaaagttat cgaggaattg agaaagcaag gctataatgt acatcaagca
     1141 agtgtaagtg catttggtag taactatgat cgcgctgtag aactttatta ttacattaaa
     1201 ggtggtcgcg tagattatgg cgcagcacat gcagctaaat acggacatga gcgctatggt
     1261 aagacttata aaggaatcat gcctaattgg gaacctggta aaaaggtaca tcttgtaggg
     1321 catagtatgg gtggtcaaac aattcgttta atggaagagt ttttaagaaa tggtaacaaa
     1381 gaagaaattg cctatcataa agcgcatggt ggagaaatat caccattatt cactggtggt
     1441 cataacaata tggttgcatc aatcacaaca ttagcaacac cacataatgg ttcacaagca
     1501 gctgataagt ttggaaatac agaagctgtt agaaaaatca tgttcgcttt aaatcgattt
     1561 atgggtaaca agtattcgaa tatcgattta ggattaacgc aatggggctt taaacaatta
     1621 ccaaatgaga gttacattga ctatataaaa cgcgttagta aaagcaaaat ttggacatca
     1681 gacgataatg ctgcctatga tttaacgtta gatggctctg caaaattgaa caacatgaca
     1741 agtatgaatc ctaatattac gtatacgact tatacaggtg tgtcttcaca tactggtcca
     1801 ttaggttatg aaaatcctga tttaggtaca tttttcttaa tggatacaac gagtagaatt
     1861 attggtcatg atgcaagaga agaatggcgt aaaaatgatg gtgtcgtacc agtgatttcg
     1921 tcgttacatc catccaatca accatttatt aatgttacga atgatgaacc tgccacacgc
     1981 agaggtatct ggcaagttaa accaatcata caaggatggg atcatgtcga ttttatcggt
     2041 gtggacttcc tggatttcaa acgtaaaggt gcagaacttg ccaacttcta tacaggtatt
     2101 ataaatgact tgttgcgtgt ggaagcgact gaaagtaaag gaacacaatt gaaagcaagt
     2161 taaattcatc ttctgaattt aataggctat gtaaatcgtg ctgttatcat ggcacatcag
     2221 atataagtag catcacagtg ttgaatttca aaatagtaaa gtgaaataaa gcgcctgtct
     2281 cattagcgaa aactaaaggg acaggcgtat ctgtttatga gcttaataaa ttgtatgaat
     2341 aatatggttg atcgaataac tgtttatcat gatgataaat tgagtttttt aaaataatga
     2401 tatattacac cattgttata gcgtttaaga aatcaacaac tttacgataa atagtgattg
     2461 cttcgtcatt aggtctacga tcaaaatcat gctcgttttt attcacgcgt tcaaatgttg
     2521 aatgtggaac atgattcatg atatgttcgc tttcctcaac gggaacatca taatcgccat
     2581 tacaatgcgc aatgaaaaca ggtggaagtg ttttaagttc atctggtgca atattatatt
     2641 ttgaatcagt ataatcagca atgttaatca tatttatcca tttacctgtg ccacgtgcat
     2701 aaacgtagat taaaaaacgt tgtgcgattt gatcttgaac aaccggtgtt ggtgaagtga
     2761 gttgtgcaat cattgtttcg tttatgcttt gagctatttt tgcgtaataa ctattagttg
     2821 ttttaaaagg ttcagtgttg atgcgactat aaccataaaa atcaataaca ccatcaatat
     2881 ctctgtctcg tgcaattaat agacttaaat atgcacctga tgatctgcca aaggtaaaaa
     2941 tagggcaatt agaatattgt gattgaatcg catcgaatga tgcgtagaca tcctcaataa
     3001 tgcaatcgag acttacttct ggtaataaac gataacttag ttgaattaaa tcgtaatgtt
     3061 ccgtaaggat atcgatatac tgtggggata aatcgttagc tttaccgaac attaatccac
     3121 caccgtggat gtagacaata acgccttttg ttggttgatt ttttgcttta ataattgtgt
     3181 aaggtaatgc aaatgcatct ttagtaatta ctttatattt aatttcagtc acgatttaat
     3241 aggctcctta tttttgatat tgatgtcatt ataacactgt cttaaatttc catgaaaaat
     3301 agtcttaaga cgatgagtca tgataattct gttccaattg acgtaaagcg tcacgggtat
     3361 gcttctttag accttcccca taatccatca ttttaacaat atctttaaaa gcagcatgtg
     3421 gaatggctaa atcttctaaa tctgccatag aaaattcaag attgatatca tgtggtcgct
     3481 gttcagcaag tttatgcaca aagtcaggtt ctgtgacaaa aggcgaagac atgccgacca
     3541 tatctgcatg ttgtaaagca tctaaagcag actctggaga attaatcccg ccacttgcaa
     3601 ttaaagggat acgacctgct aaatgttcat agacaatttg gttaactggt cgaccgaaat
     3661 gatcacctgg tgtacgagac gtattttgat aaatatgtcg accccagcta gcgattgcta
     3721 agtattggat gtttgaaacg tccatgaccc aatcgattaa ttggttgaac tcgtcaatgg
     3781 tatatcctaa atcactgcct ctggtttctt ctggcgttgc tcgaaatcct aaaataaaat
     3841 tgtcaggtgc ttctttatca atcacttctt gtaccgcacg cataacttct aaacataatc
     3901 ttgcacgatt ttttaatgag tcggcaccgt aatggtctgt acgtctattt gaaaaagttg
     3961 agaaaaatgt ttgaatcagc aaacgttgtg caatcgaaat ttccacacca tcaaaacctg
     4021 ctttaatcgc gcgtaatgta gcatcgcgat actgctgaat gatgctattg attttctcat
     4081 gagacatggc gataacaacg tgttcaatcg gtgaatgcaa agtcatagga cttggtccat
     4141 acacctttcc aaaatttaaa atggcttgat ttgaaaaacg accagcatgc gctagctgga
     4201 taatagcgag gctaccatgt tgtttcatcg tagatgccat gttagttaat ccagggatac
     4261 aagcatcatg atcaatatta aagccatatt caaacaattt accataaggt tcaatgtaag
     4321 cagcgccggt gacttgcatt ccagctgaat tagagcgacg tgcagcataa gccaagtctg
     4381 cttttgtaat atagccttct tttgttgatg cgtttacggt cattggtgat aatacaaagc
     4441 gattcgaaat tttgatgcca ttaggtaagt ggattgattg taaaagtggt ttgtatcggt
     4501 acatactatg attccttttc tattcaatat tgttttcaaa gtaccatgga aagaatgaat
     4561 aatcaatgat gaacagtctt gatagaatag aattggtaca tggaaaacat ttttaaaatt
     4621 gaactaatga atggcatttg taggtctgaa aatatgaata tgaaaaagaa aaataaaggc
     4681 gaaaagatat aaaagttaat tgaaaaacgt tatcatatac gtgggtatat gaagagggaa
     4741 tggtattaag aacgctaaaa tgttatgtcg gtttgacatg acaggataag tttggagatg
     4801 acggattggt taaattaagc gtattagact atgccttaat agatgaaggt aaggatgcac
     4861 aaaaggcatt gcaagattca gtgacacttg caaaattagc agatcgactt ggctttaagc
     4921 gaatttggtt tacggaacat cataatgtac cagcgtttgc gtgtagtagt ccagaacttt
     4981 tgatgatgca tacattggcg cagacaaatc acatacgagt tggctctggt ggtgtgatgc
     5041 tgccgcacta tcgaccttat aaaattgctg agcattttag aatgatggca gcgttatatc
     5101 caaatcgtat tgatttaggt attggtaata atccaggtac tactatggta aagcaagctt
     5161 tagatggaat aaatcctaca tatgatagtt acgatgaatc gatttcgtta ttacgtgatt
     5221 atcttacaat aaaggataaa ccaagtgcgc atacgttagg tgtccaacca cacattgatc
     5281 attttccaga aatgtggtta ttaagtagta gcgcaacatc tgccaaaata gctgccgaac
     5341 taggtatagg gctttctgtt ggaacatttt tgctaccaga tataaatgcg atacatgcag
     5401 cgaaggataa cattgatatt tacaaaaaac atttccaagc atcaacgatt aaaatggacg
     5461 caaaggtgat ggcatctgta tttgtcattg tagcagataa cgaagcggaa gtagcagcat
     5521 tgcaacatgc cttagatgtt tggttattag gtaaattaca atttgcagaa tttgaacatt
     5581 ttccttcagt agacacagca caaaagtata agcttaatga tcgagacaaa gagatgattc
     5641 aagtacatca agcacgcatc attgcaggca cacaagaaca ggttaaagca caattagatg
     5701 atttcattgc tacgtttgaa gttgatgagg tgttagtagc accgcttatt ccaggtattg
     5761 aacagcgttg taaaacatta aaattactcg cggaaattta tttgtagcat tttaaataga
     5821 agagaaagga tgaagataag atgaaaaagt tagccaatta tttatgggta gaaaaagtag
     5881 gagatttgta tgtgtttagt atgacacctg aattgcaaga tgatattggg acagtaggtt
     5941 atgttgaatt cgtaagtcca gatgaagtta aagtggatga tgaaattgtg agtatcgaag
     6001 catcgaaaac ggtcattgat gtgcaaacgc cattgtcagg aacgattatt gagcgaaata
     6061 caaaagcgga agaagaaccg acaattttaa actctgaaaa accagaagaa aattggttgt
     6121 tcaaattgga tgatgtcgat aaagaagcat tcctagcatt accggaggct taaatggaaa
     6181 cgttaaaatc aaataaagcg agacttgaat atttaatcaa tgatatgcgt cgagagagaa
     6241 atgacaatga cgtattggta atgccatctt catttgaaga tttgtgggaa ttatatcgag
     6301 gcttggcaaa tgtcagaccg gcattacctg taagtgatga atatttagct gtacaagatg
     6361 ctatgttaag tgatttgaat catcaacatg ttacggattt gaaggatttg aagccgataa
     6421 aaggtgacaa tatctttgtt tggcaaggtg atatcacgac gttaaaaatc gatgctattg
     6481 ttaatgctgc aaatagtcgt tttctaggat gtatgcaagc taatcatgac tgcattgata
     6541 atattattca tacaaaagcg ggtgttcaag ttcgacttga ttgtgcagag atcattcgac
     6601 aacaagggcg caatgaaggt gtaggtaaag ccaaaaaaac acgtggatat aatttgccag
     6661 caaagtatat aattcatacg gttggtccgc aaatacgtcg attgcctgtt tcaaagatga
     6721 atcaggactt gttagctaaa tgttatctta gctgtcttaa attggctgat caacatagtt
     6781 taaatcatgt cgctttttgc tgtatatcta caggtgtatt tgcttttcct caagatgaag
     6841 cagcagaaat tgctgttcga acagtagaaa gctatctcaa agaaacaaat tcaacattga
     6901 aagtcgtgtt caatgtattt acagataagg atttacaact gtataaggag gcattgaacc
     6961 gtgatgcaga gtagtaaatg gaatgcaatg tctcttttaa tggatgaaaa gacaaagcag
     7021 gctgaagtat tgcgtactgc gattgatgaa gcagatgcga tagtgattgg aattggtgca
     7081 ggcatgtctg catctgacgg atttacatat gtaggagagc gttttacgga aaatttccca
     7141 gattttattg aaaaatatcg cttctttgat atgttgcaag cgagtttaca tccttatggc
     7201 agttggcaag agtattgggc atttgagagt cgttttatta cattaaatta tttagatcaa
     7261 cctgtaggtc agtcttatct cgcattaaaa tccttagtgg aaggtaaaca gtaccatatc
     7321 ataactacga atgcagacaa tgctttcgat gctgctgaat atgatatgac tcatgtattt
     7381 catatacaag gggagtatat actgcaacag tgtagtcagc attgtcatgc tcaaacgtat
     7441 cgcaatgatg atttaattcg taaaatggtt gttgcgcaac aagatatgct tataccttgg
     7501 gagatgattc caagatgtcc aaaatgtgat gctccaatgg aagtgaataa acgtaaagcg
     7561 gaagttggca tggttgaaga tgctgaattt catgcgcaac tacaacgtta taatgctttt
     7621 ctagagcaac atcaagatga taaagtgttg tatttggaaa ttggaattgg ttatactaca
     7681 ccacaatttg tgaagcatcc ttttcagcgt atgacacgta aaaatgaaaa tgcaatttat
     7741 atgacgatga ataaaaaggc atatcgcatt ccgaattcaa ttcaagaacg taccatacat
     7801 ttaactgagg atatctcaac attgattaca acagcactcc ggaacgacag tacaacgcaa
     7861 aataacaaca ttggagagac agaagatgta cttaatagaa ccgattagaa atggagaata
     7921 tattactgat ggtgcgattg cactggctat gcaagtttat gttaaccagc atatcttttt
     7981 agatgaagat attttattcc cttattattg tgatccaaaa gtggaaattg gacgttttca
     8041 aaatactgct atagaagtga atcaagatta tatagataaa cacagtattc aagtagttcg
     8101 ccgagatact ggtggtggcg ctgtgtatgt tgataaaggt gccgttaata tgtgttgtat
     8161 tttagaacaa gacacttcaa tttatggtga ttttcaacga ttttatcaac cagctataaa
     8221 ggcattgcat acattaggtg caacagatgt gatacaaagc ggtagaaatg atttaacatt
     8281 gaacggcaaa aaagtgtcag gcgccgcaat gacattaatg aacaatcgta tttatggcgg
     8341 ttattcgcta ttacttgatg ttaattatga agcaatggat aaagtgttaa agcctaatcg
     8401 caaaaagatt gcatcgaaag ggattaaatc tgtgcgcgca cgtgttggtc atcttagaga
     8461 agcactggat gaaaagtatc gtgatataac cattgaagaa tttaaaaatt taatggtgac
     8521 gcagattttg ggaatcgatg acattaaaga ggcgaaacga tatgaattaa cggatgcaga
     8581 ttgggaagcg attgatgaat tagctgataa aaagtataaa aattgggatt ggaattatgg
     8641 gaagtcacct aaatatgaat ataatcgaag tgaaagatta tcatcaggta ctgtagatat
     8701 taccatttct gttgaacaaa atcgtatcgc agattgtcgt atttatgggg atttctttgg
     8761 acaaggtgat ataaaagatg tggaagaagc attacaagga acaaaaatga caagagaaga
     8821 tttaatgcat cagttaaagc aattagacat cgtttattat tttggtaatg ttacggtaga
     8881 atcattggtt gagatgattt taagttaata ttgtttaggc gttaatgtca caccctcaat
     8941 gaatatgtgt atttgctttt aaaatggtga ctatgtagtt atgtaatttg attatacgat
     9001 tgaaaatgaa tttagttaat tagcaaagac atcatattta tagcgtttgt gtttattagt
     9061 gattgggtat ttgaaaatgg ttaatgatag tttttaaagg aataaattca ttttcattta
     9121 tataagtaag attaaatact agctactaaa gtctatttat atacgtcaaa atataatgac
     9181 tgctataatg agtaatcaat agacacaaag aggagattat gtgatgaata ataaagtatt
     9241 agtaaccggt ggtacagggt ttgttggcat gcgaattatt tcacgattat tagaacaagg
     9301 ttatgaggta caaacaacga tacgtgattt aagtaaagct gataaagtaa ttaaaacaat
     9361 gcaagacaat ggcatttcca cagagcgatt aatgtttgtc gaagcggatt tatcacaaga
     9421 tgaacattgg gatgaagcaa tgaaagattg taaatatgtc ttgagtgtag catctccggt
     9481 gtttttcggt aaaacagacg atgcagaagt gatggcgaag cctgccattg aaggcataca
     9541 acgtatttta agagctgcag aacatgctgg cgtgaagcgt gtggtgatga ctgcaaactt
     9601 tggtgcagtt ggttttagca ataaagataa aaattcaatc acaaatgaaa gtcattggac
     9661 aaatgaagat gaaccaggct tatcagtata tgaaaaatca aaattgttag ctgaaaaggc
     9721 agcgtgggat tttgttgaga atgaaaatac aatagtagaa tttgccacaa tcaatccagt
     9781 tgcaattttt ggaccatcat tagatgcaca cgtttcagga agtttccact tgttagaaaa
     9841 cttattaaat ggttcaatga aacgtgtacc gcaaattcca ttgaatgttg ttgatgtgag
     9901 agacgtagct gaactacaca ttttggcaat gacaaatgaa caggctaatg gcaagcgatt
     9961 tattgctaca gctgatggac aaattaattt gttggaaatt gcaaaattaa ttaaagaaaa
    10021 gagacctgaa atagctcaaa aagtttctac taaaaaatta ccagacttta ttttgagtct
    10081 aggtgctaaa tttaatcatc aagctaaaga aggtaaactt ttattagata tgaaccgcaa
    10141 tgtaagtaac gaacgtgcaa aaacattact tggttgggaa ccgattgcga cacaagaaga
    10201 agcaatttta gcagcaattg atagtatggc taaatttcat ttaatataat acaaaaacac
    10261 cgtccataag taagtggctt tcctcttgat aaaagaggca ttcactcaat atgtgacggt
    10321 gttttatata tttacgttaa cactattgct gtggcttttg acggcctttt aataaaattg
    10381 ctgtcgcacc tacgataata ataaatagaa tcgcaccaaa taatcccata tattttactg
    10441 cgttaccgaa cacgataccg acagttaaaa agtctgtatc tgagaatgtt gttgcagcac
    10501 cacctaattc gcctaaaaat ggcaagaata ataatggtaa aaacgtgatt aggataccat
    10561 ttagagcggc gccagcaaca gcacctttaa taccgcctct tgcattaccg aatacagcag
    10621 ccgttgcacc taagaagaag tgtgcaacta cgccaggtaa aatgacgacg ccaccaaata
    10681 agaataagat aaacataccg atgacacctg taataaagct gacaaagaat ccaattaata
    10741 ctgcattttg tgcataaggg aacacaatag ggcagtctaa tgcaggttta gaatttggta
    10801 caagcttttc agaaattcct ttaaatgctg ggacgatttc agctaagatt aaacgaacgc
    10861 ccgttaaaat aataaataca ccagcagcaa atgtcacacc ttgaattaaa gaaaagacaa
    10921 taaagttttg accatcacta atagattcgt gtacataact aacgcccgca aataagcatg
    10981 cgatgaagta aagtaatgcc attgtaatcg agatactaat tgtactttct cgtaagaaac
    11041 ttaagccttt tggaaattta atctcttccg ttgatttaga cttacctttg aataattgac
    11101 ctacagcacc tgcggcaaag taactgattg agccaaaatg acctaacgct acttggtcat
    11161 tccctgtaat ttttcgcatc gtaggttgga gtaatgcagg taatactgcc atgattaatc
    11221 ctaatacgag tgcgccgata acaattgtta gccagccttt aatatgactg actgttaaaa
    11281 tgattgctaa aaacgcagcc atgtaaaatg tatgatgacc tgttaaaaag atatatttta
    11341 aattagtgaa gcgagcaatt aaaatattaa caatcatgcc acagaccatg atgagtgcag
    11401 ctgttgttcc aaaatctttt aaggctagtg agacgatagc ttcgttgtta ggtacgatac
    11461 cttgcacacc aaatgcgtgt tggaatattt tgccgaatgg ttcaagagat cgaacgacga
    11521 catcagcacc tgcacttaaa attaagaagc ctaatatcgt tttaatggtt cctgaagtga
    11581 tcgttgcggc aggttttttc tgaacgatta aacctataaa ggcaatcagt gcaacaagaa
    11641 tggctggttg acttaaaata tcgactataa aattaaggat tgcttgcata ggtcgtacct
    11701 cctttaaatc atgttaagtt gttgtaattt ttctgagagc ttttgttgta attcagcttt
    11761 atctaaaata ttatcaagaa ctaagacatc ccctaggcgt tcggcatttt cagctaaatc
    11821 tctaccacaa ataaacaagt cagccatctc tggacttgct gtcataatgt cactatgttc
    11881 aacttcgata tcagatggtg cattaagttg cctaagtgct tcttgtgcgt tcatttctac
    11941 cataaaacta cttcctaaac cgtggccaca tactactaaa attttcatat taatcatgct
    12001 cctttaaaat gtttttaatg tcttgtgcgt tttttgcagt taatagttgc tggactgttt
    12061 ggttatcgcc cagtacggtt gctaaatttt gtaatacaga taagtgtgaa tgattgtcga
    12121 tggcactcaa tacaaaaatg agagatgcgt agtgatcttc atcacaaaat gccacatgtt
    12181 gattcaactt taatagactt aaaccaactt gatgtacgtc attgttcggt cttgcatgtg
    12241 caattgcaat ttcaggtgcg ataacgatat aaggtccaag ttcattaacg ctatcaatca
    12301 ttgcttgaac atagccttgt tcaataattt gttcttgtag taatggctga gaagctatag
    12361 ttatagcttc agtccaatca tttacttgtt cttttacaat gatgcgtgtt gttgacaaaa
    12421 tgtctaatga cacgttatta aacctccttt gtcatagtta aagcaatgtg ttgtttaatt
    12481 ttaaaaatat tcccatctaa gaaatcttgt cgatataagt cgttgcttaa gcattcgctt
    12541 aactgtccca atgcctttaa atgtgcattg gggtggtccg ttgctaatgt aattacaagg
    12601 tgaacgggat cgttagcttt actaccaaag ataatccctt cagtgaaata tgttagtgcg
    12661 aaacctacac cattctgtac ataatcagta ccagcgtgaa taagtgcaat atgtggacta
    12721 atgaccatat atgacccgaa ttgttcaaat tgttttaaaa ttgcagctgt ataatttgaa
    12781 tagacaatgc catcattgat taaaggttgc acagccactg caattgcgga ttcaattgat
    12841 aatggttgtt catttataat gatgcgatgt tcaggcaata aatctgcgag tgacttgcca
    12901 tcagttgcca ttttcatgac tcgttgttct cttgagtcat tgataatttg attcaatttt
    12961 tgacgagatt gttgattgat aaatggatcg acatgaataa ctggtacagc tgatatttca
    13021 caaggtactg ttgaaatgac ataatcaatg ttatcttgca ataatcgact ttcttccaat
    13081 tgataaatgg aataggcatc ccaaatgtga aactcaggat acagatgatt tagttttgat
    13141 tttaaaagtt gcgacgtgcc tataccagaa ccacatagta agacaacctt aatcattgat
    13201 tgtttatgtg ttgcaacacg ctctatactt gatgcgaagt gaattgtaat gtatgttaat
    13261 tcatcttcgt tgaagcgaat agcagcatct tgttcaattg ggctaatatg cttgctaacg
    13321 gcttcaatga tttgaggata gcgacgcata acttcttgcc tcaaaggatt aggttgtagc
    13381 atatcgtatt taatacgatg tatagctggt ttgatatgtg tgatcagact ggtatgcaac
    13441 ttgttgtctt ttgacatatc aatgcctaat tcttggctaa cacaagtgat caattcatgt
    13501 atattttgcg ataaatcatg gtattcaaag gtaattgaag atgctgtatg ttcggtcatt
    13561 ttagagccta gtaaatgtaa cgtgataaag ataatttcag actctggaaa tgtgacatta
    13621 caactgcgtt ctaagttttc tatcattttt gaagcaatag catactgatt agtatgtcgc
    13681 catttatcaa tttcattgat aggtatatcg aacgaaaaat tttcatttaa acgctgaatg
    13741 gcaatgagta tatgatagat taagccatcg atagccgact gaactaaatg ataattttca
    13801 ctatttaatg tcttaataat ggcacggcga accaatgcga ttgattctga attaaagata
    13861 tccgcctcta taaaaggtgc agcttgtttc atatattgat gtataaagtg tgcatacgct
    13921 ttacgataat gatcttcctc accaataaca ttgaatcctt tattgtggac ataatttaac
    13981 tttaaatggt attgatctag ttgggcttga atcattttaa tatcatctgc aattgtccga
    14041 cgcgaaacat taacatcttg cgcaagttgc tttgttgaaa caggatcggt tgtttcgaat
    14101 aactttaaag cgatatgtgt gagtcgttca tcttttgaaa aatgaatttg atttgttaga
    14161 ttgtgctcta attcatttaa tagtgtcgcg tgagctgttg ttactttgat gcctgcagct
    14221 ttattacggc tgacttggta atgataagtt tcagcatatt gctcaatata tgctatatca
    14281 tattgaatgg tacgaggtga tacaccaagt tgattagcaa tggtattgat tggaataaac
    14341 gtttgctcat gaattaaaag atacaaaatt tcgatttgtc tataacttaa caacgtaata
    14401 tcctcctatt tgtaattgta agcgatttct taaaaacgta gatatgcaat ctctttcata
    14461 ttttaatccg aaaaattgca tatcaaaatg tttatggcgc aagattttat aggaactttt
    14521 aaaataaatt agatattcat gttgacaatt taaaaatgtc gcagtatatt tagttagaca
    14581 tctaacgaaa tggtggtgca ataaatggaa ttcacttatt cgtatttatt tagaatgatt
    14641 agtcatgaga tgaaacaaaa ggctgatcaa aagttagagc aatttgatat tacaaatgag
    14701 caaggtcata cgttaggtta tctttatgca catcaacaag atggactgac acaaaatgat
    14761 attgctaaag cattacaacg aacaggtcca actgtcagta atttattaag gaaccttgaa
    14821 cgtaaaaagc tgatctatcg ctatgtcgat gcacaagata cgagaagaaa gaatatagga
    14881 ctgactacct ctgggattaa acttgtagaa gcattcactt cgatatttga tgaaatggag
    14941 caaacactcg tatcgcagtt atctgaagaa gaaaatgaac aaatgaaagc aaacttaact
    15001 aaaatgttat ctagtttaca ataaatggta agtgtgactg gtagaaatca gtcactttat
    15061 ctttaatatt atagttagat atctaattgt tagtaagcta attattggaa aagacaagga
    15121 gtattgaaca atgaaagacg aacaattata ttattttgag aaatcgccag tatttaaagc
    15181 gatgatgcat ttctcattgc caatgatgat agggacttta ttaagcgtta tttatggcat
    15241 attaaatatt tactttatag gatttttaga agatagccac atgatttctg ctatctctct
    15301 aacactgcca gtatttgcta tcttaatggg gttgggtaat ttatttggcg ttggtgcagg
    15361 aacttatatt tcacgtttat taggtgcgaa agactatagt aagagtaaat ttgtaagtag
    15421 tttctctatt tatggtggta ttgcactagg acttatcgtg attttagtta ctttaccatt
    15481 cagtgatcaa atcgcagcaa ttttaggagc gagaggtgaa acgttagctt taacaagtaa
    15541 ttatttgaaa gtaatgtttt taagtgcacc ttttgtaatt ttgttcttca tattagaaca
    15601 atttgcacgt gcaattgggg caccaatgat ttctatgatt ggtatgttag ctagtgtagg
    15661 cttaaatatt attttagatc caattttaat ttttggtttt gatttaaacg ttgttggtgc
    15721 agctttgggt actgcaatca gtaatgttgc tgctgctctg ttctttatca tttattttat
    15781 gaaaaatagt gacgttgtgt cagttaatat taaacttgcg aaacctaata aagaaatgct
    15841 ttctgaaatc tttaaaatag gtattcctgc atttttaatg agtatcttaa tgggattcac
    15901 aggattagtt ttaaatttat ttttagcaca ttatggaaac ttcgcgattg caagttatgg
    15961 tatctcattt agacttgtgc aatttccaga acttattatc atgggattat gtgaaggtgt
    16021 tgtaccacta attgcatata actttatggc aaataaaggc cgtatgaaag acgttatcaa
    16081 agcagttatc atgtctatcg gcgttatctt tgttgtatgt atgattgctg tatttacaat
    16141 tggacatcat atggtcggac tatttactac tgatcaagcc attgttgaga tggcgacatt
    16201 tattttgaaa gtaacaatgg catcattatt attaaatggt ataggtttct tgtttactgg
    16261 tatgcttcaa gcgactgggc aaggtcgtgg tgctacaatt atggccattt tacaaggtgc
    16321 gattatcatt ccagtattat ttattatgaa tgctttgttt ggactaacag gtgtcatttg
    16381 gtcattatta attgctgagt cactttgtgc tttagcagca atgttaatcg tttatttatt
    16441 acgtgatcgt ttgacagttg atacatctga attaatagaa ggttaaatat ttcgtacact
    16501 tctggctgag tatatttcgg tcggaagggt atttttcgaa aaaaataaat atatgatacg
    16561 attatgaaaa aataaagtga gattggcata tgtacaaatc taaaatactg ttgaaatata
    16621 tttttagtga agaatcagaa gttaaagatt taactgaaga aaaatataat caagattacg
    16681 aagcattaac atttagcttt aaagaggaaa catatcaaag taggttagct aagaaaacac
    16741 cgactaaagc agggtatttt gttacatgtt ggacgaaaga cgagaacaat tgtaatcaac
    16801 catactcgaa agaggcattt gcagattact taatgattat agttattgat gaagaattaa
    16861 gcggttattt tttatttcct agggaattat tggtagaaaa aggtatctta actacatttg
    16921 aacataaagg taagatggct tttagagttt atcctaagtg gtgtaatcaa ttgaataaaa
    16981 cagcagggca aacacaaaag tggcaatgta aatatttttt gaatactaat aaaaagtcat
    17041 atggttgaac ttataagaga tgaatatatc atctttcgat aagataacca tatgacttta
    17101 ttttttatca tttaatgatg aacggtttct tgtcctactt tattccaagt gaggataaag
    17161 ctcaacattg caaacacact gattgctgtt aataaaataa aaccgacatc ccatccgaat
    17221 ttatcaacta cagcacctaa gacgatgttg gccattacag caccaaacag ataaccaaat
    17281 aatcctgtta atccagctgc tgtgccagct gcttttttag gtacataatc taatgcttgt
    17341 aaaccaatta acataactgg tccatatatt aagaaaccaa tggcaattaa tgagacattg
    17401 tctaaccaag cattgcctgg aggatttaac caataaatta atacaaatac tgtgacacct
    17461 aacataaaga agaaacctgc aggtccacga cgacctttga ataatttatc agaaatgtaa
    17521 ccacataata atgtaccagg aattccagcc cattcgtata agaagtatgc ccaacctgat
    17581 gcttttaagt cgaaatgttt ttcttcactt aagtagactg gcgcccaatc aagtacacca
    17641 taacgcacga aataaacaaa tatatttgca aaggcaattg cccataccca tttattgttc
    17701 agtacatatt taaataaaat ttcttttgta gttaattctg tttctaatgt tttcttatcg
    17761 cttgtagcaa agtcgttttt ataaatttcg attggaggta aaccttgaga ttgaggtgtg
    17821 tctctaatca atacgtatga aattgcggca atgataagtg ctaagagtgc agggtaaatg
    17881 aatacacctt cgaaaccttt taaataacca aagttgataa atgctgttgt tgtaataccc
    17941 caagcagcaa taggtgccat aatacctcca ccaacattat gcgcaacgtt ccaaagggca
    18001 gtcttacttc cgcgttcact tacactaaac cagtgaacga gaacacggcc tgaaggtggc
    18061 cagcccatac cttgaaacca tccatttaag aataatagga caaacataat accgatacct
    18121 gatgtaaaga acggtacaaa tcccattaac aaattgacga tagcagtgag tgctaatcca
    18181 agaactaaga atatccgagc attgctccga tcacttacag tacccataaa gaacttacta
    18241 aatccatatg cgatggaaac agcagaaagt gcaaaaccta gttccgcttt tgtaaaacct
    18301 tgctcttgca atgctggcat cgctaatgaa aagtttttac gtaataaata gtacccagcg
    18361 taaccgatga aaataccaag aaatacttgg agacgtaatc gtttataggt atcatctatc
    18421 tgattttctg gcaaaggctt aatatgcttt gcaggtttaa gaaaattcat aaaatcctcc
    18481 ttaatatgta tttatatgca ttttgtgcga taaacttata ttagacatgt atgaaagcgt
    18541 tgttaatata cttttgtgaa atcttgcata tttagttatt gaagtatttt ataaacattt
    18601 agaaaaaata aattgctaca cataaattta taattatgca attattatgt cgttatacat
    18661 actagctgtg tatctacgaa tgagtacaaa catattttta tttgcagaaa aggggtaaat
    18721 ggcatataac tatctttttt atgtaagttg gtataaaatt tatactaata ggagggatag
    18781 tatgaatata gtagggcatc atcacatatc catgtataca aaagatgcaa aacgtaataa
    18841 ggatttttac acaaatgtcc ttggattacg attagttgaa aagtctgtta atcaagacaa
    18901 tccttcaatg tatcatttgt tttatgggga cgaagtaggt acagccggaa caattttaag
    18961 cttttttgaa attccccatg cgggtcataa gcagccaggt actgaaacga tttatcgatt
    19021 ttctttatta gtaccaaatc aagcggcact tcattatttt gaaaaacgcc ttgataataa
    19081 tggtattacg tctgaacgtt tgtactatct tggacaagaa ggtgttgtct ttaaagatga
    19141 agacgactta gaaatcatat tgcttgttaa tgatagtttt gaagtaccac atcaatggca
    19201 acataacgct tatagtgaaa tacctcaagt atatcaaatt ttaggaatag ggccagtcga
    19261 attaagagtt agaaatgcag cgcgtacggt agaatttttg gaaaatgtct taggttatcg
    19321 caaaagagat aataaatcat tcgatgtgct gacattagca ccacaaggtt tatattcgga
    19381 ttttgtagtt attgagcaac agggacaacg tgaaagacct ggacgaggtt atatccatca
    19441 tattgcaatt aatacaccac aaatgagtga cttagatgca atttacaaga aattacaaca
    19501 acaaacacaa agtaattcag gtataattga tcgctatttc tttaaatcat tatactatcg
    19561 ccataattca attatgtatg aatttgcgac tgaagcgcct ggatttacta ttgatacacc
    19621 tcttgaacaa ttaggaagtc aattgaactt gcctgacttt ttagaagcag aacgtgaaca
    19681 aattgaaagt aagttacacg aaatataaag gagaatgttt aatggccaaa ttagaaatga
    19741 ataaaaatac gcctcttgag tttggtttgt attccttagg tgatcattta ttgaatccat
    19801 ttaaaggtga aaaagttagt tatgagcaac gtattaatga aattattgaa gcaagtaaat
    19861 tagcagatga aacaggtatt gatgtttttg cagttggtga aagtcaccag gagcatttta
    19921 caacacaggc acatacggtt gtgttaggtg caattgccca agcgacaaag catattaaag
    19981 tttcaagttc ttcaacgatt attagtgcaa cagatcctgt aagagtattt gaagacttcg
    20041 cgacattaga tttgatttct catggtagag ccgaaattgt agctggcaga gcatcaagaa
    20101 caggtatttt tgacttgttt ggctatgatt taaaagacta tgatgaattg tttgaagaaa
    20161 aattaggttt acttttagag ttaaataaaa ctgagcgtat tacttggtct ggaaaatatc
    20221 gtccagaact tagaaatatg aaaatattcc caagaccaat cgataatata ttgccaatat
    20281 ggcgtgctgt tggtggtcca cctgcaagtg ctattaaagc gggaaaacaa ggtgtgccaa
    20341 tgatgattac aacccttggt ggcccagcaa tgaactttaa aggttctata gatgcttatc
    20401 gtcaagcagc aactgaagca ggtttcgatg cttcgcataa gtctttacca gtaagtacag
    20461 cgagtctgtt ttatacagct gaaacaactc aggatgctat gagagaattt tatccacatt
    20521 tgaatacagg gatgtcattt attcgtggtg ttggttatcc gaaacagcaa tttgctaatt
    20581 cgtcagatta tcgagaagcg ctaatggttg gaagcccgca acaaattatt gaaaagatat
    20641 tgtatcaaca cgagttgtat ggtcatcaac gttttatggc acagcttgat tttggcggtg
    20701 tgccatttga aaatgttatg aagaatattg agttaattgg caacgaaatt ataccggcga
    20761 ttaaaaagca tttatcaaaa taggaggggc gtcatcatga atattgtatt attgtcaggt
    20821 tccacagtag gttctaaaac gagaattgct atggatgatt taaaaaatga actagaagtc
    20881 atcaatgagg gacatcaaat agagttgatg gatttacgag aacttgaatt agaatttagc
    20941 gttggaaaga attatctaga tactacagga gatgtatata aattaacgac gtcgttaatg
    21001 caggctgatg tgatttttat tggttttcca atttttcaag cttccatccc tggtgctttg
    21061 aaaaatgtgt ttgatctact tccagtcaat gcttttcgtg acaaggtaat aggacttgta
    21121 gcgacagcag gttctagtaa acattattta attcctgaaa tgcatttaaa accaatattg
    21181 agttacatga aagcacatac gatgcaaacg tatgtattta ttgaagagaa agatttttca
    21241 aatcaacaaa ttgtcaatga tgatgttgta tttcggttaa aagcgttggc acaattcaca
    21301 atgcgaactg ccaaagtaca acaacaagtg tttgaagaag aaaacaacca atacgacttt
    21361 taaagtataa aaataagacg ctcggcacac taaatttgta tgtgtttgag cgtcttttat
    21421 attaactata tagccagtga acgacgataa aggcaagtga tgacaagcat attgaggtaa
    21481 taatgattgt cataagcggt ttaagtgcac gatttttaag atctttaaat gcaacattta
    21541 accctaaagc aaccatggcc attaataagc aaattgttga tatagtattt aaaatattta
    21601 acaatactga tggaatagtt acatatgtat tcactaaggc cataatgaca aatccaatta
    21661 aaaagtatgg aatgcttatt cgacccttgc tagatgattc tgatgaacgg aaacgcataa
    21721 ttaaaataag tacgatggtt aatggaatca gtaagaatac tctaccaagt ttaccaagaa
    21781 gtgcaatttt aagtgcatca ctaccaccaa agccaccagc taagacaacg tgcgcaattt
    21841 catgaagact aacaccagac caagcgccat aaacatttgt cgtcattgaa aagatagcgt
    21901 agatagctgt atatataagt gagaatatcg taccaatcaa tgcgatgata ccgatactaa
    21961 tagctgtatc cttttcacgt gatttgaata ttggagcgac tgcggcaata gcggcagcac
    22021 cacaaacgcc tgtgccgaca cctagtaata atgcgatgtt tttgtcacca tgcaacagtt
    22081 tgttgacaaa gagcatcatt acaatactga aaataacgac acctacatcg atggctaata
    22141 gtttactacc ttgaccgata atatcgaata tattgagttt aagtccatat aggatgattg
    22201 caaatcttaa taaatattta gatgaaaacg taatacctga gctatattgt tcaggatatc
    22261 ctctaaagtg acgatataga atagcgatta atatcgcgat agttaatgcg ccaaccttat
    22321 ctaagattgg caatttagct gctaaaaagc taaataatgc gactataaat gttaatgata
    22381 gtccaatcat aaaatgctta tttttcaatg atgccatgag cagtgcctcc tttaatagca
    22441 ttttagcact gttttgtcgt atttttaaat ataaatttgg aatggttaat aaagtagtga
    22501 ttaaattaag ttgtgtgata ggtatcaagg acatcaacca aagtaatagg cactacaacg
    22561 cttattggcg gggccccaac aaagaagctg acgaaaagtc agcttacaat aatgtgcaag
    22621 ttggggatgg gccccaacat agagaaattg ggtccgtaat ttctacagac aatgcaagtt
    22681 ggcggggccc caacaaattt aagatacaat gcgtaatatt aatatgttat tattatgata
    22741 atttacagaa ttatatgaaa aatgaatgag gatgtgatgg tatgtttgga atgaaagtga
    22801 atgaacaaat aacattaaaa attttagaag ctcatgacac agaagcgctt ttcaatttag
    22861 tcaatcgttc aagaaattca cttagggaat ggttaccttg ggtagatgca actgagcaac
    22921 catcagatac gcgtgcattt attaaaagag gacttttgca atttgctgat ggtaatggat
    22981 ttcagtgtgg catttggtat gaaggaacgc tagttggtgt catcggttta catgaaatta
    23041 atcacatgca cagaaaaact tcattagggt actatttaga taaacaattt gagggtcatg
    23101 ggattatgac acaagcagtt gaggcattga taaagtattg tttcgaagag cttgacttaa
    23161 accgaattga gattagtgcc gcagttaata atgaaaaaag ccgagctatt cctgaaaggc
    23221 tgggatttac tagagaaggt atgttacgtg acaatgaatt actaaatggt atttattcat
    23281 cgagttacat ctatagttta ttaaaatcag aatacgacca aaaatgacaa attagactta
    23341 caaaagagtg atgacattta aaatggcatc gctcttttat ttaatttttg aaaataaaag
    23401 gttgttgaca gtattatttt ataacaatat aatgattttg ataattatta tcaactagat
    23461 aatgttcatg ggaggatgcc ttaaaatcat cattttaaga gccacgtatt atcttggcat
    23521 ttagggagtg tgagatagta cgaatgaggg catgtctatg gggataatag aatttctata
    23581 atgaggtgtc aaaatgaaaa agttaacaac gctattatta gcatcaacgt tattaattgc
    23641 tgcatgtggg aacgacgata gtaagaagga tgattcaaag acatcgaaaa aagatgatgg
    23701 tgttaaagca gaattaaaac aagcaacaaa agcatatgat aaatatactg atgaacagtt
    23761 aaatgaattt ttaaaaggta cagaaaaatt tgttaaagcg attgaaaata atgatatggc
    23821 ccaagcaaaa gcgttatatc caaaagttcg tatgtattat gaacgctctg aaccagttgc
    23881 agaagcattt ggagatttag atcctaaaat tgatgcacgt cttgcagata tgaaagaaga
    23941 gaaaaaggaa aaagaatggt caggatatca taagattgaa aaagcattat acgaagataa
    24001 gaaaattgat gatgtgacta aaaaagatgc acaacaatta ttgaaagatg caaaagaatt
    24061 gcatgccaaa gctgatacat tagatatcac accaaaatta atgttacaag gttctgttga
    24121 cctattaaat gaagttgcaa cttctaaaat cacaggtgaa gaagaaattt attcacatac
    24181 agatttatat gattttaaag cgaacgttga aggcgcacaa aaaatttatg acttatttaa
    24241 acctatttta gagaaaaaag ataaaaaatt aagtgatgat attcaaatga acttcgataa
    24301 agtgaatcaa ttattggata aatataaaga taacaacggc ggttatgagt catttgaaaa
    24361 agtatcgaag aaagaccgta aagcatttgc ggatgctgtt aatgcattag gagagccact
    24421 aagtaaaatg gctgtgatta ctgaatgaca aattatgaac aagttaacga tagtacgcaa
    24481 ttttcaagac gtacattttt gaaaatgtta ggtattggcg gtgccggtgt tgcaattggc
    24541 gcaagtggtg ttggtagcat gtggtctttc aaatcaatgt tcaatacacc agaagatccg
    24601 gaaaaagatg cgtatgaatt ttatggtaaa gtgcaaccag gcattaccac acccacgcaa
    24661 aaaacatgca atttcgttgc gttagatttg aagtcaaaag atagagatgc aattaaggca
    24721 atgtttaaaa agtggacggt tatggctgat cgtatgatgg atggtgatac agttggcaag
    24781 acgagtaaca atcctttaat gccaccagta gataccggtg aatcgatagg attaggtgca
    24841 agcaagttaa cgattacctt tgggattagt aagtctttga tgaagaaaat tgggttatct
    24901 agtaaaattc ccgatgcctt taaagattta ccgcattttc cgaatgatca gttaatagac
    24961 gattacagcg atggtgatat tatgattcaa gcatgctcaa atgattcgca agtatccttt
    25021 catgcggttc ataatttagt tcgtccattt cgagatattg ttaaggtacg ttgggcgcaa
    25081 tctggtttta tctctgctaa aggtaaggaa acacctagaa atttaatggc atttaaagat
    25141 ggaacaatta atcctagaaa gagtaatcaa cttaaagatt atgtgtttat tgatgacgga
    25201 tgggcgaaac atggaactta ttgtgttgtc agacgtattc aaatacacat tgaaacgtgg
    25261 gatcgtactg cactggaaga acaagaggct acatttggtc ggaaacgaca tagtggtgcg
    25321 ccgttaacag gtgggaaaga gtttgatgaa attgacttaa aagcgaaaga tagtcatggc
    25381 gagtatatta ttgataaaga tgcccatacg agactagcga aagaagcaaa tacgtcaatt
    25441 ttacgtagag cctttaatta tgttgatggt acggatgacc gcacaggtaa cttcgaaaca
    25501 ggcttgttgt tcattgcttt tcaaaaagcg acaaaacaat ttatcgatat acaaaataat
    25561 ttaggtagta atgataaatt aaatgaatat attacacata gaggttctgc ttcattttta
    25621 gtattaccag gtgttagtaa gggaggatac cttggtgaaa cattatttga ctaaatttgt
    25681 agcaatgcta ataactgctg ctatggtgtg tagctttggg ttactgaaaa gtcaggcagc
    25741 agaacaacaa agtattagtg atgtatatag tgtgataacg gatgcgaaat ctgcactttc
    25801 taataattcg atatcgaatg acaataagca gaaagcaatt gagcaagtgg taagtgcagt
    25861 taagaaatta tcgcttgaag ataatagtga aggtaatgct gtcaaatcag atgtgagaaa
    25921 gcttgaagat gcaaaagcga atgataatca aaaagataca ctttcgcaat taacgaagtc
    25981 attaattgct tatgaagaga aattggctag taaagatgcg ggttctaaaa ttaaactatt
    26041 gcaacagcaa gtcgatgcca aagatgctgc gatgacaaaa gcgattaaag ataaaaataa
    26101 agcggaatta gaatctttga acaatagttt gaatcagatt tggacaagta atgaaacagt
    26161 gattcgcaat tatgacgcaa atcaatatgg acaaattgaa gtcgcattat tacaacttag
    26221 aattgcaatt cataagtcac cattagatac ggcaaaagtg tcacatgctt ggacaacttt
    26281 taaatcaaat attgatcatg tcgataaaaa aagtgatacg tctgcaaatg atcaatacca
    26341 tgtatcacaa ttaaatgatg cgttagagaa ggcgattaaa gctatcgacg acaatcaatt
    26401 gtcggatgct gatgctgcgc ttacacattt tatagaaatt tggccgtatg ttgaaggtca
    26461 aattcaaact aaagacgggg ctttgtatac gaaaattgaa gataaaatac catattatca
    26521 aagtgtatta gacgaacata ataaatcgca tgtcaaagat ggtttagtag atataaacaa
    26581 tcaaattaaa gaggttgttg gccatagtta tagctttgtc gatgtgatga ttatcttttt
    26641 acgtgaagga ctagaagtgt tattaattgt aatgacattg actaccatga cgcgtaaagt
    26701 caaagataat aaggggactg caagtgtgat tggtgctgca attgccggac ttgtactgag
    26761 tattatctta gcaattacgt ttgtagaaac tttagggaat agtggcattc ttcgtgaaag
    26821 tatggaagcg ggattaggta tcgttgcggt catattaatg tttatcgttg gtgtttggat
    26881 gcacaaacgt tcaaatgcga aacgctggaa tgatatgatt aaaaatatgt atgccaatgc
    26941 gattagtaat ggtaatttgg tattgttagc gacgattggt ttaatatctg tgttgcgtga
    27001 aggtgttgag gttatcattt tctatatggg gatgataggt gagctagcga caaaagattt
    27061 tattattggt attgccttgg ccatagtgat attaattgtc tttgcggtat tatttagatt
    27121 tatcgtcaga ttgataccta tattctatat atttagagta ttgtcgatct ttatttttat
    27181 tatgggattc aaaatgcttg gcgtaagtat tcaaaagtta caattattag gtgcgatgcc
    27241 aagacatgtt attgaaggat tcccaacgat taactggttg ggcttctatc caagttatga
    27301 accattgata gcacaagctg cttatattat ggtagttgct atcttaatct ttaaatttaa
    27361 aaaataaaaa acaggccgag tgcctgtttt tttgttgctg tattgcggat tatcgtagca
    27421 ttgttgagat gtggatatcg ctgcatggtt ggttctcgtt gtattgcaga tatcacggtt
    27481 taatccgatt ctgtattcta attaacgcaa gtgcagtatt gatataatct gaggtgctac
    27541 attgttcttt tattggctat tgtgatgttg ctgcattgtg agtatcacag cattaattcg
    27601 tataaggtta atgtgttggc ggtttgcctc ggcatgtgaa cttaacaatg aacatactga
    27661 actcaaagag caatatgagt ggcaatgtaa gtaatatatt taatgttaaa tcgggtggtg
    27721 caatgatact tgccagcaca aagcaagcga aataaatata ttttcgataa tgcttcaatg
    27781 atgtgatatc tataagaccg aattttgcaa gtcctatgaa taatattggc aattggaata
    27841 aaatgccaaa agtgaaaagc caacggataa gttcaactaa atacgcttta aagccaatga
    27901 caggcgaaat gtttaaagtt aatgataatt ttaacgcgaa ttgaatgatt attggaaagc
    27961 caacataaaa tgcaaaagcg acgccagcac agaataataa cacgctgaaa aaactatatt
    28021 tataaataaa ttgacgttca ttattatgta atccaggtgc aatgaatgcc cacaattgat
    28081 aaaacatgac tggtgaaatg aaacaaaacg cgatgaaaaa tataatcatc acgtatattt
    28141 ggatcatttc tgtgaatgaa aatgcatgta aggacacatg tgcacgggta atatacgtta
    28201 tgaatggtgt catccaccaa aatgatgaaa catatacgac gatgactgtg acgacgaacg
    28261 acaatagtat tttaactaaa cgatgacgta gctcgctaaa atgaactagt aaggtgtggt
    28321 cagtgctatt gctctcgctg ttgtttcgat tccttactgg gtgtgtcgtg agactctttg
    28381 tctaaatctt ctgttgcaga tttaaattct tttaaagtag aaccgatagc actaccaaat
    28441 tgtggtaatt ttttcggacc aaaaataatt aaagcgataa tgctaatgac gacaagactt
    28501 gttgggcctg tgatgcctaa aataaaagtg ttagttatca tgataatcaa cctcactcat
    28561 aagtagtata tagtatttac tttataccga acgataatga ttatcaataa attttataaa
    28621 ataattaaaa tgttgctgta aatacaaatt actgaggtat taattctgaa ataatagctt
    28681 gttctttcaa gtcgatataa gtacaagtac cagcttgaat atcgatatgc catttataaa
    28741 tgccagcttc agccatttca tcacaaaatg tttcgaaatc tgtttgtcct tgttgatgtc
    28801 tagttaatac atcttgaact attgttttgt ttgatttttg agcaacagga tgattacttt
    28861 tcacagatga cgtaacgata tcatcttctg attgatgtac gtatgttgca gtgccatctt
    28921 gaatgttgac gatattgtaa gtcatcccca tatctttaaa agctttgaat agttttggaa
    28981 agtcaacacc agtaaattgt tgatgtgctt gttgaattgc agataatgta aatgccataa
    29041 tagtctcctt tgaatggttt gatttagtga aaatgcgtgc atcatactaa aattagaatg
    29101 ctgttaatta tgcaggaagg tgtaacgaaa gtaaacaatt atgacttgta ggcgttgagg
    29161 tgttgtttca aaatgtcata cataggtgga caatcatatt tttttgtgta gatttgacaa
    29221 atatagttgt caaaaagaca aatatcattt acattaggac taaggagttg atgcgatgtg
    29281 cgtaatcgat tgaaagagtt acgagcacga gatggcttaa atcaaacgca gcttgccaaa
    29341 ctagctggtg tttcaagaca aaccatttcg ctaattgagc gaaacaattt tatgccatca
    29401 gtattaacgg caataaagat tgctcgcatt ttcaatgaaa cggtggaaac ggtttttatt
    29461 attgaggagg atgaagcatg aaaatactaa gatatatcgg atatctttta ctgggtggaa
    29521 ttgtaggggg tatcattggt ggaattttag gtaactttga tggattgggt attgagaact
    29581 tgacgtttgc gacttataac aatgtcgttg taatatcgat tgttgcgact atgatcatca
    29641 tattggtaga agccattgtt ttgatgaatc aaagacgtgc attgaagtat aagcgacttg
    29701 tagatgaaga ggtagatatc gatgcaacag atcaatatga attgcttgcg aatcgttatg
    29761 ttttaaatgg aagtatatta agcgttatac agacaattat tgcctttgta gtgttactaa
    29821 tttttgtggt agggcaagct gaagcaaatg caatgctatt ctttttaata ccattttttg
    29881 ctagtgctat tttcaataca caatttacac tgtttaatag aaaatttgat gacagaatgc
    29941 caaaaattgc agataagaat tacactgaaa agcgattgga aatattggat gaaggtgaac
    30001 gccatataga attaattgca ttatttaaaa catatgcgat caacttatca atattgatac
    30061 tagccattat ttttataggg tcttattcaa ttgctacagg aattaatcaa agctttagtt
    30121 tgctacttat cattgctatt ttcatatata acgcctttag ctatttattg aagagaagac
    30181 gtttttatta aaattaaaca aagaggagca tggatatgac aacattgtta aacgtagatc
    30241 gtgtgaacaa acaatacaaa gattcggatt ttaaattgca agatgcatct ttaacgattt
    30301 ctactaatga gacagttgga ttaattggga aaaatggctc aggtaaatcg acattaatta
    30361 atattctagt aggcaatcga cataaagata acggtagtat tgcatttttt ggagaagaac
    30421 atgctgcgga tgatgtcgaa tataaagaac acataggtgt tgtgtttgat gatctgagag
    30481 tacctaataa attgactatt aaagatattg ataaagtatt tcaatctatt tatacgactt
    30541 ggaatagtca aaaattcttt gatttaatca aatatttcga gttaccacta caaactaaaa
    30601 ttaaaacttt ttcaagaggg atgcgaatga agatagcttt aacgattgcg ctttctcatg
    30661 atgtgaagtt attaatctta gatgaagcaa ctgcaggtat ggatgtttct ggaagagaag
    30721 aagtaatgga actattagaa gattttgtcg ctcaaggtgg aggcatctta atatcatcgc
    30781 atatttctga agatatagaa caattagcgg ataaattagt gtttatgaaa gatggacgaa
    30841 tgattttaac tgaacagaaa gatatacttt tagcacaata tggaattgtt acgacaggag
    30901 ataaagatgt tgaaattcct aagcatttaa tcattgcttc tagattgtca aaggggaaat
    30961 atcaaatttt agttaaagat tatgcagaaa ttgaaaatgc agagccttta aaacacattg
    31021 atgacgctac gaaaatcata atgcgaggtg aagtataatg aaaggtatgt tcctaagtag
    31081 tttttatgca acgagaaagc aaacatatat ttattttata gtcgctatca tagctgcggg
    31141 atactttgca gtatttaatc cgttgatgag ttcggcaatg gctggggtta tgttaatcac
    31201 acccattact gataatatta aacatgaaaa agactcaaga tggatgtatt atgtatctac
    31261 tttaccggtt aaacgtagtg attatattaa gtcatacttt gccttttatt taatcttatt
    31321 cggtgcaagt ttaatgattg gattagttgt gactacgatc gtgacccaaa gtgtgatgat
    31381 tggtattatg tcaggtttaa tgagttttgg tatcataggg gcatactcta tcattttccc
    31441 attgacattt aaatttggcg ctgaaaactc taatgtcatt atgatagttg catctatcct
    31501 actacttttt tctttcgtgg tctttttctt tatatatggt atggttagtg gtgcatcggc
    31561 attagaattt gaaaaaatta gcactgaagg atggttagtt gtcatagtat atgcggtcat
    31621 tggtatcgtt ataacaagtg tttcttatat attgtctatt aaaattttta acaaacaaga
    31681 actataatca atattcttgg tgtgaagcta gtcattgaaa taatatttag agcaggtgat
    31741 aaatcaatac gattatcatc tgctcttttt ggtgttgtgg aatgaaatgc gggatgatag
    31801 gttgaggtga catctctata tcatatcatt tgtttttaga agattaggtt atgtgggaat
    31861 tgcatccttt tatagtgagg attaacgtgt atatatcatt gagtatgttg ctgaaattta
    31921 tttgcgtgta tcatttgatg attgtatagg ctaacgattt ccttggaaat atttaaaaac
    31981 ctcgatcatg tagcataact gaatccaaaa gtataatgtg aagttcgaca cttttggatt
    32041 cagttcaaat actttgaccg aggtgaatac tatttattca tgtttattac gtagacgttg
    32101 atttttaaaa taaatcagca tgatgcagaa agtcgcaacg gataccgaga taatcattac
    32161 ttggtcatgc cccttaaaga taaggctgat gatatatgat actacaagta tgcctagtga
    32221 agaagaaata tattttgcgt ttgtcagttc attatggaaa taaggcgtga ttaaccataa
    32281 tccaatatag aatattaaaa cactgatata catcatatta atttcaaaca agtcatttag
    32341 tttattgtta ttactaaaaa caattgcagc attaatcaca cctaaagcga tattgattaa
    32401 tagatgcgta tacgataaac ggaaaccgat agatgacaat ttatgattaa tgtaattttc
    32461 agtaatgatc caatatacac cgaaaagact aattaaaatc ataaattgga atatataaat
    32521 gtaactaaaa tgatcaatgc taaatgatga cgaagctaaa ccaaccagta cctcgccaaa
    32581 gataataatt gttagtaacg aaaaacgttc tactaaatgc atcatattaa caggtgataa
    32641 tacaagatat ttctgaaatg gaataagtcc tgtcgctgca atgaatacgc ctaaaaatcc
    32701 agggatgtaa tggatacttt gtggtagtac taatgataga aatgataaaa atgaaatcac
    32761 aaaggctacg ctcgcaaaag cttgacatgt acgcttatcg ccataatcta accctgtacg
    32821 tatatgtaat aaatactgta atccgatact taaatacata attgccacgc ataagaagaa
    32881 tgggaagaat gtcttttcaa agtccggata taggctgtta gataggaaga ccatgatgaa
    32941 catattaaac atcataaacg agacgtcttt gaatgtaact tgaccaaatc gatttgtaaa
    33001 aaatgtttga tgagaccaca ttaaccataa gaacaaactc atgacgatgt atttgaaaaa
    33061 taaatcagct gaaatggaac cgttttgtgt tgttaaaatc acatgtgcaa ttttttgaat
    33121 ggcatagacg aaaattaaat caaagaacaa ctcatggaat cctgcacgct tttcagctaa
    33181 atgttttggt gttaatgcat taaccataaa attttaactc ctttaagatg tgtaattaat
    33241 ttactaagta tactatttat tttttctagt gaataggggc agatttggcg atgaagtgga
    33301 aggagaggtg actgcaaggt aattgcggaa ttaacaatca tcagcgattt aatatttgac
    33361 tggagacgtc atggtaataa aaaattgatg agaaattgat ggtgaaacca gctgtgaata
    33421 gcgatgcaat gatagataga atttaattag agtcattacg cgaaatgatt aatgataatt
    33481 tgtggtaaat caaagcataa ttttgtacta tagatgagga tgatagagca tatttaagag
    33541 tgtgaaatgt taaagtgaaa ccgtttacgt ttccgattgc ccaaacaaat tacatcattg
    33601 tataatatga tttgttaaat gcataacaag aatgaaaatg taacatacgt agcaattggt
    33661 ttcataaatt ggatgttagt ggcgtattgg ttcattagac gtattagtaa taaaattgta
    33721 tatatcataa ggagatgaat atgacatgac gagagtcgta ttagcagcag catacaggac
    33781 acctattggc gtttttggag gtgcgtttaa agacgtgcca gcctatgatt taggtgcgac
    33841 tttaatagaa catattatta aagagacggg tttgaatcca agtgagattg atgaagttat
    33901 catcggtaac gtactacaag caggacaagg acaaaatcca gcacgaattg ctgctatgaa
    33961 aggtggcttg ccagaaacag tacctgcatt tacagtgaat aaagtatgtg gttctgggtt
    34021 aaagtcgatt caattagcat atcaatctat tgtgactggt gaaaatgaca tcgtgctagc
    34081 tggcggtatg gagaatatgt ctcagtcacc aatgcttgtc aacaacagtc gcttcggttt
    34141 taaaatggga catcaatcaa tggttgatag catggtatat gatggtttaa cagatgtatt
    34201 taatcaatat catatgggta ttactgctga aaatttagta gagcaatatg gtatttcaag
    34261 agaagaacaa gatacatttg ctgtaaactc acaacataaa gcagtacgtg cacagcaaaa
    34321 tggtgaattt gatagtgaaa tagttccagt atcgattcct caacgtaaag gtgaaccaat
    34381 cttagtcact aaggatgaag gtgtacgtga aaatgtatca gtcgaaaaat taagtcgctt
    34441 aagaccagct ttcaaaaaag acgggacagt tacagcaggt aatgcatcag gaatcaatga
    34501 tggtgctgcg atgatgttag tcatgtcaga agacaaagct aaagaattaa atatcgaacc
    34561 attggcagtg cttgatggct ttggaagtca tggcgtagat ccttctatta tgggtattgc
    34621 accagttggc gctgtagaaa aggctttgaa acgtagtaaa aaagaattaa gcgatattga
    34681 tgtatttgaa ttaaatgaag catttgcagc acaattatta gctgttgatc gtgaattaaa
    34741 attacctcct gaaaaggtga atgttaaagg tggcgctatt gcattaggac accctattgg
    34801 tgcatctggt gctagagttt tagtgacatt attgcatcaa ctgaatgatg aagttgaaac
    34861 tggtttaaca tcattgtgta ttggtggcgg tcaagctatc gctgcagttg tatcaaagta
    34921 taaataataa gaaaacaggt tatcacaaca gtattaatta catgttggca taacctgttt
    34981 ttatttgttt atggatttat tgggtaatat tagtcatttg atggtttaat tgcaaatgct
    35041 ctaacaggga acccaggtgc atcttttggt ttagggctga tagcgtaaat gatggcgcca
    35101 cgagttggta attgatctaa attagttaat aactcgactt ggtatttatc ctgaccaaga
    35161 atataacgtt cgccaactaa atcaccattt tttacaacgt ccacagatgc atcggtatcg
    35221 aatgtttcat gaccaacagc ttcaacacgg cgttcttcaa ttaagtactt caaagcatct
    35281 aatccccaac ccggtgcatg ttgttgtccg ttcgcatctt tgttttcaaa cttttcaata
    35341 ttaggccaac gttttgacca atcggtacga agtgcaacaa aagtgccagg ttcaatagta
    35401 ccatgctctt tttcccatgc ttctatatgc gcacgtgtta cgatgaaatc attgttgttc
    35461 gctacttctg ttgaaaagtc taatacaatt aacggcaata ccaattcttt taaatcaatg
    35521 tcttctaaat aacgtttgtt ctcgacaaag tggattggtg catcaatgtg agtaccatat
    35581 tgcgttacaa tattccaacg ttgcacatag aaaccatgat ctttaaccgt gaataaagtt
    35641 gaaacttcac ctttttcaaa ctcactaaaa cgtggtattt ctggatcaaa tgtatgcgtt
    35701 aaatcaaccc aagttgcttg ttttaaagta tttaattgtt gccataaagg atattgtgtc
    35761 ataaaatcac ccgtttttag tttattatat gataaatgct gcgattattc ttggcgttta
    35821 gctttaacag cattcacaag tacagtcaat gcatctttaa cttcttcttc ttttcgcgtt
    35881 ttcaaaccac agtcagggtt tacccagaat aatgagcggt cgatttgttg tagtgaacga
    35941 ttgattgctg tagtaatttc ttcttttgtt ggaatacgtg gactatgaat atcatataca
    36001 cctagaccga tacctaaatc ataattaata tcttcaaagt ctttaattaa atcaccatgg
    36061 ctacgagatg tttcaattga aataacatca gcatctaagt catgaatagc atgaatgatt
    36121 tgaccgaatt gagaataaca catatgtgta tggatttgag tttcatcacg aactgaagac
    36181 gttgcaagtt taaatgataa aacagcatct ttaagatatt gttcgtgata ttcagagcgt
    36241 aatggtaagc cttcacgtaa tgcaggttcg tcaacttgga taactttgat tcctgcagct
    36301 tcaagtgcta atacttcttc gttgattgct aaagcaattt gatcttgaac gattttacgt
    36361 ggtaaatcaa cacgttcaaa tgaccagttt agaattgtta caggtccagt taacatacct
    36421 ttaactggtt tatctgttaa gctttgtgca taaactgttt catcaacagt taaaggcgct
    36481 gtccatttta catcaccata aatgattggt ggttttacgg cgcgtgaacc atatgattgc
    36541 acccaaccga atttagttac taagaaacct tgtaattttt ctccgaagaa ttcaaccatg
    36601 tcattacgtt caaattcacc gtgaactaat acatctaagc cgatgtcttc ttgaatttta
    36661 atccatcgag caatttcatt ttttaagaat gtttcatatg cttcgtctgt aatgcgtttg
    36721 ttcttccaat ctgcacggta ttttcgaact tctcggcttt gtgggaatga tccaatagtt
    36781 gttgttggta aatccggtaa gttcaaacgt ttttgttgtt gttcaatacg ttgcgcgaat
    36841 ggtgattgtc ttgaagtacg cacgctttcg aaatcataat ctaagttttt gaatgattga
    36901 ttttggaaac gctcataacg tgcttttaat ttatcatatt taacactatc gttttgattc
    36961 aataggcgac gcaatgcatc taattcgtct aatttttcag ttgcaaagct taagccttcg
    37021 ccaacacttg tatctaatgt ttcatcatct aaagatactg gaacatgtaa taatgaagat
    37081 gatggttgaa tgacaagctc attagtgtgt gctaacaatt tgtcgattaa gactttttta
    37141 gcttcaatat cacttgccca tacattacga ccatcaataa ttccagcgta taatgttttt
    37201 gatttatcaa aatctcctgc ttcaatttgt ttaaggttat agccattatc atggacaaag
    37261 tctaaaccta aaccaccaac aggtaaagaa cttaagaatt taagatgtgc acgttcaaag
    37321 tatgtttgaa tgactaattt tttagcaaca ccagcttttt cgaaatagtc ataagcttca
    37381 cgtgtaatat tttcatagct ttcgctgtcg tctgtaacta agattggctc atcaacttga
    37441 atgtactcag cacctgcatc aattaatgat tcaaacactt ctttataaag tggtaataac
    37501 gttttaactt tttcttcaaa agtttggtga ccgccttttg ataatttaac aaaagtaatc
    37561 ggaccaacaa tgacagggtg agcgttaacg tttaaagatt gggcatattt aaagcgatct
    37621 aataatacat tgcgactcac tttaggctca acattgtccc attcaggtac gatgtaatga
    37681 tagttagtgt taaaccattt tataagtgca cttgcaacat ggtctttatt accgcgagca
    37741 atatcaaata ataaatcatc atcaatagtt cttccttgga aacgttcagg gatgatgttg
    37801 aataataatg acgtatctaa tatatggtca tataaagaga aatcaccaac tgggatgcta
    37861 tctaagtgat agtacttttg taataataaa ttttctttat gtagatcagt taatgtttga
    37921 tctaattctt ctttagaaat cttctttgcc caataacttt cgatggcttt tttccattct
    37981 ctttttctac ctaatcttgg gaatcctaag tttgatgttt taattgttgt cataatattg
    38041 cctccttgtg agcagtaata gattttgagt atgctgcaag ttctaatgaa tcttcgacat
    38101 tttgaaacgg tgtgataatg tataaaccat taaaatattc atgaacagta tcgattaaat
    38161 cctttgaaag cttaagactt agttctcgtg ttttggcttt atcatcttta actgcttcaa
    38221 attgttgtaa aatttcatct gacatcttga ttcctggcac ttcattatgc aaaaagagtg
    38281 cgtttttgta acttgcgata ggcataatgc ctatgaaaaa tggtttgttc aagtgcttag
    38341 tggcatggta aatttcaatg attttctctt tgctgtacac gggttgtgtt ataaaataag
    38401 acattccgct ttctatcttt ttctctaatc ttttgacggc accatctaat ttacgaacat
    38461 tagggttaaa ggcgccagcg atgttgaagt gtgtacgttt cttcagcgca tcaccgtcag
    38521 tgttaatacc ttgattaaat cttagagcga gttcagttaa tcctttagaa ttaacatcat
    38581 agacattggt tgcacctggt aagtgaccaa cttttgaagg atcaccagtt atggctaata
    38641 tttcgttaac gccaatgagc gataatccaa gtaaatggga ctgcaagccg attaagtttc
    38701 ggtctcgaca tgtaatatgt acgagtggtt caatattgta atattgctta attaagctag
    38761 cagcagcaat attgctaatt ctgacagttg ccaatgaatt atctgcgagt gttaccgcat
    38821 ctacattagc tttatcaagt ttagcgatat tttcaaaaaa tctatccgtg tctaaatgtt
    38881 tcggtgtatc caattcgata ataacggttg gacgttcttg aaccttagat gttaatgatt
    38941 gtctaacttt attttgagat ggattgaaaa gtgctttcgt tggtatcgga atcacttttt
    39001 tgtcattaac aggtttaagt gtctgaatag attctttaat aaatttgatg tgctctggcg
    39061 ttgtaccaca gcaaccacca attaaacgaa caccttcgcg aattagattt tgagcaactt
    39121 gaccgaaata ttgtgcattg tcactatact taaattcact attttcaata tctaataagc
    39181 tggcatttgg ataacaagat aagaatgcgt gctctggtaa ttcaatatgt gtgaaagact
    39241 cttgcatatg gtgcggacca tgatgacaat tgagtcccac gatgtttgca ccacattgaa
    39301 cgagttgttt taatccttca ttgattgcct gaccattaac taagtaattt gtgtttgaag
    39361 cggttaattg agcaatgatt ggaatgtcgt atttctttct cgttcgtgaa atgacatttg
    39421 ttaactcttc taggtcgtaa tacgtttcga aaagtagcgc gtcaacgcct tcttcaatta
    39481 aggtgtctat ttgaatttca gtatgataaa gaatagtttg taagctgata tcctcttgtt
    39541 tgatacctct aaacccacca actgtgccta atatatacgt atctttattt gctgcttttt
    39601 ttgcgatgcg aacggcggct tgatgtattg ctttaacttt atcttcaaga ccgaatcgtt
    39661 ttaacttttc aaaatttgca ccataagtat tggtttgaat gacatcagca ccggcttcaa
    39721 tatatgaacg atggatgcgt tcaactttat ctggatggct aagattatat gcttctggac
    39781 aggtgtctaa tccttcagag tataaaatgg ttccaatagc gccatcagct actaaaacat
    39841 tatctttcaa ttgtgtgagg aattgactca ttgaatgcct cctttaatgc gtatttgatg
    39901 tctgcaatga gttcatcagg atcttcgaga ccaacactta atcggaatag accgaaagtg
    39961 ataccacgtt cttgtctcac ttcttcaggt agtgcagcgt gagacattgt tgctggatgt
    40021 gaaaggatcg tttcaacacc acccagactc actgaaacga gtggtaatgt cagtgcatcg
    40081 acaaattgtt gtgctttaga ctcatcagct aaacgaaagc caataacggc accgccattt
    40141 ttagcttgtt ctaaatgagc agtagtgagt cccggataat aaacttctga aatttcatct
    40201 tgctttatta aaaatgacac gattttttgc gcgttttcga cagattgttt aaatctgatt
    40261 ggaaatgttt ttaaatgttt agcaagtgtc cagctatctt gagcagataa catattgcct
    40321 gtaccatttt gtattaaata aagagcttca ctaattgcct cattattagt aatgacagca
    40381 ccagcaatta aatcgctatg tccacttaaa aattttgtag cactatgaat gacaatatca
    40441 gcgccaagta ataaaggtga ttggcctaac ggtgtcataa atgtattgtc cacagctacc
    40501 agtagttcat gcttttcggc tattttagaa acagctttga tatcagtaat tttaaaacag
    40561 ggattcgatg gtgtttcgat ataaattaat tttgtgtttg attgaatggc accctcgatt
    40621 tgttcgagct tggtagtatc tacggttgta aattcaatat taaatcgatt caaaatttgc
    40681 tcagtgaggc gaaaagtacc gccatataca tcatcgggta agatgacatg atcaccagat
    40741 ttgaaagtca aaagtactgc tgaaatagca gcaatacctg atgcaaaagc aaaagcgaat
    40801 tttccctgtt ctaatcgtgc taacttctct tctaaaagtt cacggttagg gttgccactt
    40861 cgtgcataat catatttaac atcgccacca agacttgttt gatggaatgt tgaagaatca
    40921 tagagtggtg ggttagctga atgatattcc acacctctac gccaatcgaa tatcacttct
    40981 gtctcttttg aaagtgtcat acaatctctc caatctgagc tttatctaat gcttggatga
    41041 tatcgcgttc gatgtcttca taattttcaa cacctagtga taagcggatt aaatactcat
    41101 caatgccacg tttatctttt tcagcatctg gcatatcaac atgtgtttgg gtgtaaggga
    41161 aggtcactaa tgtttcagta cctcctaaac tttctgcaaa aatacaaatg tctaaatttt
    41221 ctaataattt agcgacgcta taggccttgt taagtcttaa actaagcatg ccagtttgcc
    41281 cgctatatag tacttcgtca attgcttgaa gtgactgaca ttttttagca agttttctag
    41341 cgtttgattg cgcacgctca atgcgtaaat gcaaagtttt aagtccacgt aacaacaaat
    41401 aactatctat tggtgaaagt gttgcgccag tcatgttgtg aaaatcaaac aactgttgcg
    41461 cgagtgattc atctttgacg gttacgacac ctgctagtac atcgttatgt ccaccaatat
    41521 atttcgtagc tgaatgtaag actatatcag caccttctgc taatggtgtt gaaagataag
    41581 gtgttaaaaa agtattgtcg ataattgaca ataagccttt agctttacaa agttgatagt
    41641 acggctttac atcaatagca atcatttgtg ggttagatat tggttcaatg aataatgcaa
    41701 ctgttttatc agtgatttct ttttcaactt gttcataatc tgtaaaatca acgtacttaa
    41761 atttgatatc gtattgttgc tcgtaaaatt caaataatct aaatgtgcca ccatataaat
    41821 cgaatgaaac taaaatttca tcatgaggtt taaatagatt acatattaat tgaatggctg
    41881 acattccact tgatgtagcg aatgatgcaa taccatgctc aagtttggca aaacaggttt
    41941 caaatgttga gcgtgtagga tttttagtac gtgtataatc aaatcctgtc gattgtccta
    42001 gttttggatg tttgtaggca gtagataaat ggattggatt cgctatagca ccggttgaat
    42061 catcagttaa tgtgatttgg gctaactgtg tatccttcat attaagaccc tcctataaga
    42121 aaaaataaaa agcttccgtc cttcgtaccc gaatgaatcg gataaaaagg acgaaagctt
    42181 atgtttcgcg gtaccacctt tatttgttat tccatcgctg aaataacctt attcagtacg
    42241 cattaaaagt aaatatgctt actgaacaat tatcacaatt aaagtcagta agtaaggata
    42301 tagtaatgtg ctatcccata cttattaaca aaaaatcgtg cgtaaagaat ccagtacgcc
    42361 atttaacatc aatgttaata ctgtatcgct ataacgggcg aacccgtaga cacctcatat
    42421 tggcatcaac actccaaggc cattttcaaa cacgctttca aaatcttctc tcagctacta
    42481 aagactctct gtataagcag ggtgtgtttt actttcctct ttattgtgtt tacgtttcat
    42541 taaactatta taagatatta attagcttac agagtaaaaa aagatttgtc aacaattatt
    42601 cagaaaattt tgatttaaaa gttaatttgt ttgtgaaatt gtaattggta tcttgaagtt
    42661 gaaaaatgaa ttatttttta aataaagtgt ggttaatggt tgtctgactc atttagaata
    42721 cataaaatat atttaactgt tgttatcaaa taaaaagtga tgtgagtgaa ttgtcaaaaa
    42781 gtgaagatca acgtattact aaaacaaaag atgaacaaat taagcaaata gatatatcgg
    42841 atatcaaacc gaatccgtat cagccccgaa aaactttcga tgaaaatcat ttaaatgatt
    42901 tggcagattc aattaagcaa tatggaattt tgcaaccaat tgtgcttaga aaaacagttc
    42961 aaggttatta cattgtagtt ggtgaaagaa ggtttagagc ttcgaaaatt gctggtctaa
    43021 aatacgtatc agcgattatc aaagatttaa cagatgaaga tatgatggaa ctggcggtca
    43081 tcgaaaattt acaacgagaa gacttaaatg cgattgaaga agctgaaagt tatcaacgtt
    43141 tgatgacaga tttgaaaatt acacaacaag aagtagcgaa gcgattgagt aagtcgcgcc
    43201 cgtatatagc gaatatgttg aggttattac atttgccgaa aaagattgct gacatggtaa
    43261 aagatgggcg actgacaagt gcacatggac gaacgttatt ggcaattaaa gatgaacaac
    43321 aaatgcttag gttagcgaaa cgggttgtta aagaaaagtg gagtgttaga tatttagaaa
    43381 accatgttaa tgaattaaaa aatgtttcgt caaagtcgaa aacagacaaa gtagatataa
    43441 ctaagcctaa atttataaag caacaagaac gacagttgcg agaacagtat ggtaccaaag
    43501 tagatatatc aataaaaaaa tcggttggta aaatctcatt tgagtttgat tcacaagatg
    43561 actttgtgag aataattgaa caattaaatc gtaggtatgg taaatagtta cacaatttta
    43621 tataataact ctttgtgaat ctatagatat attgatagtt atctatattc tttgaagatg
    43681 atgcgacttc agtaggttgg aagaatacaa caatattaat aaaattattt gagactatat
    43741 agaagggagt aacgctaatt atggatcaag ttatgagtat tatttcatcg ctattcgaac
    43801 cattaactaa aattgaaaca tatgaaaata ttgcaactaa aatcgctatg attgttattt
    43861 atattatcgt agccctcata gttattaaaa tactgaataa aatgattgaa cagggattta
    43921 agattcaaaa taaaagtaaa aagagtaaca aaaagcgctc taaaacttta atatctcttg
    43981 ttcaaaatgt agtgaagtat atcgtttggt ttatagttat tacgacgatt ttaagtaaat
    44041 ttggcattag tgttgaaggt gttattgcaa gtgctggtgt cgtaggttta gcagtaggtt
    44101 ttggtgcgca gaccattgtt aaagacgtta ttactggatt tttcattata tttgaaagcc
    44161 aatttgatgt aggtgattat gttaagataa acaacggtgg tacaactgta gcagagggaa
    44221 cagttaaatc aattggactc cgttcaacac gtattaatac aatatctgga gaacttacaa
    44281 tcttgccaaa tagtagcatg ggtgaaataa cgaattactc aattacgaat ggaacagcaa
    44341 ttgttaaaat tccagtatct gtagatgaaa atattgataa tgttgagaag aaattaaaca
    44401 aattatttac atctctgcgt agtaaatatt atttatttgt aagtgatcct gttgtgattg
    44461 gtattgatgc ctttgaagat actagagtaa tattaagggt ttcagcagaa acaataccag
    44521 gtgaaggttt ctctggtgcg cgtattattc gtaaagaagt acaaaaaatg tttttacaag
    44581 aaggtatcaa gacaccacaa ccaataatga caccttttaa tcatagtgaa aacggtgttt
    44641 agtagtttat aatacatgga ggtcatattt aatggcgtca aaatatggaa taaatgatat
    44701 agtagaaatg aaaaaacaac atgcgtgtgg aaccaatcgt tttaaaatta ttagaatggg
    44761 tgctgacatt agaattaagt gtgaaaattg tcaacgaagc attatgattc cacgtcaaac
    44821 gtttgataaa aaactaaaaa aaatcatcgt atctcaggat gatacacaaa gataggagaa
    44881 tgattcatgg ctttaacagc aggtatcgtt ggcttaccaa acgttggtaa atcaacatta
    44941 tttaatgcaa ttacaaaggc gggtgccttg gcagcaaact atccgttcgc aactatagac
    45001 ccaaatgtgg gtattgtaga agtgccagat gcaagactac ttaaattaga agaaatggtt
    45061 caacctaaaa aaacattacc tactacattt gaatttacag atattgccgg aattgttaaa
    45121 ggtgcttcta aaggtgaagg tttaggtaat aaattcttat cacatattag agaagtagat
    45181 gcaatttgtc aggttgttcg tgcttttgac gatgataatg taacacatgt agctggtcga
    45241 gttgatccaa ttgatgacat cgaagttatc aatatggaat tagtacttgc agacttagaa
    45301 tcagttgaaa aacgcttacc tagaattgaa aaattggcac gtcaaaaaga taagactgct
    45361 gaaatggaag tgcgtatttt aacaactatt aaagaagctt tagaaaatgg taaacccgct
    45421 cgtagtattg actttaatga agaagaccaa aaatgggtga atcaagcgca attactgact
    45481 tctaaaaaaa tgctttatat cgctaatgtt ggtgaagatg aaattggtga tgatgataat
    45541 gataaagtaa aagcgattcg tgaatatgca gcgcaagaag actctgaagt gattgttatt
    45601 agtgcaaaaa ttgaagaaga aattgctaca ttagatgatg aagataaaga aatgttctta
    45661 gaagatttag gtatcgaaga accaggatta gatcgattaa ttagaacaac ttatgaatta
    45721 ttaggattat caacatattt tactgctggt gtgcaagaag tacgtgcttg gacatttaaa
    45781 caaggtatga ctgcacctca atgtgctggt atcattcata ctgattttga acgtggattt
    45841 atccgtgccg aagtaacaag ttatgatgac tatgtacaat atggcggcga aagtggcgct
    45901 aaagaagcgg gcagacaacg attagaaggt aaagaatata ttatgcaaga tggcgatatc
    45961 gttcatttca gatttaatgt ataaacgata gagtgaagtt aattaaatag tatatatgta
    46021 gaagaggcgg aatcaattgt tcgcctcttt taattatgcg tataatttat taaaagaatg
    46081 gaatgatttt actcgcgtaa ataatatctt gagtgctgaa aaattgtttg ccttcgccag
    46141 tataagcagg ctctaaaaca agattagcct ttgcacaata aagccattca ggatgaatgc
    46201 cactattaag tatctcttgg aattcttgaa aatctttaga ccaatcaata tttaaattca
    46261 ttccttacca cctcacaacc attatagaac atgcgttcgc ttttgtgaag tgtattttta
    46321 aaaatatcat agaaaatttc aaatgaatta atgtcaaaaa tgatatgaag agtagtttga
    46381 attattattg taaaaagtaa tggcgcatga tataattctt tattgtgagt aatgaaaatt
    46441 attccttgct tatctgtttt aagattgata agccgtatag accacaagga ggtgcaaata
    46501 taaaatgaga acatatgaag ttatgtacat cgtacgccca aacattgagg aagatgctaa
    46561 aaaagcgtta gttgaacgtt tcaacggtat cttagctact gaaggtgcag aagttttaga
    46621 agcaaaagac tggggtaaac gtcgcctagc ttatgaaatc aatgatttca aagatggctt
    46681 ctacaacatc gtacgtgtta aatctgataa caacaaagct actgacgaat tccaacgtct
    46741 agctaaaatc agtgacgata tcattcgtta catggttatt cgtgaagacg aagacaagta
    46801 ataattagag ggggcgttta aatgctaaat agagttgtat tagtaggtcg tttaacgaaa
    46861 gatccggaat acagaaccac tccctcaggt gtgagtgtag cgacattcac tcttgcagta
    46921 aatcgtacgt tcacgaatgc tcaaggggag cgcgaagcag attttattaa ctgtgttgtt
    46981 tttagaagac aagcagataa tgtaaataac tatttatcta aaggtagttt agctggtgta
    47041 gatggtcgct tacaatcccg taattatgaa aatcaagaag gtcgtcgtgt gtttgttact
    47101 gaagttgtgt gtgatagcgt tcaattcctt gaacctaaaa atgcgcaaca aaatggtggc
    47161 caacgtcaac aaaatgaatt ccaagattac ggtcaaggat tcggtggtca acaatcagga
    47221 caaaacaatt cgtacaataa ttcatcaaac acgaaacaat ctgataatcc atttgcaaat
    47281 gcaaacggac cgattgatat aagtgatgat gacttaccat tctaataaaa attaacgaaa
    47341 ttaaagcgaa aaaattatca aaggaggcac acaatcatgg caggtggacc aagaagaggc
    47401 ggacgtcgtc gtaaaaaagt atgctatttc acagcaaatg gtattacaca tatcgactac
    47461 aaagacactg aattattaaa acgttttatc tcagaacgcg gtaaaatttt accacgtcgt
    47521 gtaactggta cttcagctaa atatcaacgt atgttgacta cagctatcaa acgttctcgt
    47581 catatggcat tattaccata tgttaaagaa gaacaataat atttaatata ttataaccct
    47641 cgtaggcata ggcttatggg ggattttttt atgtttggga tatagaaaag ggacagaaaa
    47701 gggcaaaaaa ggatgatgtg aatgttttgt gttcggaatt tgcacaaaga tatgtttata
    47761 ttgcaaaaat aatatgaatt tagatgcata aaaaaagaac tacgcatttt aaataaaatg
    47821 catagctctt ctttttcttg catacgaatt aaaataactc gcgagaccta taagtctctt
    47881 tcctcactag atagtttata cttttggtct gttgaagtca ataattttat ctaaagctat
    47941 aaaaaatctt ttgatagcta atgcattatt ataatagctt tcgtttcttt tatatcgctt
    48001 ttgaagttgg tccaaatcgt gatatcttgc ttggataatt gcattactac aaacttgatt
    48061 atgtaattct agcgtagcga aagtatctat gaaatttttt attccgaaca tgtttctaga
    48121 tatgcctata ttattccctt tttcaaataa atattgagga gatctactgt cataatttag
    48181 atttgctatg atgggttggt tatgagctga tttgtttctt atatttttaa ctaaaggcat
    48241 taaaatatta gcaactctca attcttcgtc attgtacttc ttgtaataga agttgagaaa
    48301 cgaaacgaat tgacctagtt gcatgaattc aatgcaaacc catgcgggtg gattttgata
    48361 gtatttattc aacttctcgg gtagttgtcc tcgtttattc atatgcttga atatttcgtt
    48421 tttatttttg attttggttt ccataacttc ttctggtgtt cttgaatttg tgtcaaaatt
    48481 tgaattgcta tatgatttat caatacataa gaactcatct attattttat aaccatcttc
    48541 ttggttattt tctgttatta gttttaagac tagacacttt aaactatgtt caatatctaa
    48601 agttaaatgc aacattgtgt atcttaattt catatctata gttgctaaat ctgataaata
    48661 agcaaattct atgaaatagc cgccattctt tttttcgaaa ttttttcgga aataagctag
    48721 tttgaagaag taattatttt ttctaagaat ttcatttgct ttttcggtgt caataatatt
    48781 aaaaaatata ttcatctgtt ttaatttcgc tatttgctca tcaaaattga gcataggctt
    48841 aatttctgct agtatatcat ctttttccaa ttttaactcc ccaatcgttc aaatttattc
    48901 atcatatctt tcgccatctg attagtaaca tgtgtgtata tctctagatt ttttataatc
    48961 tgaatgacct acatgctctt gcattgcttt taagttaatt cctaattgag caagtgtaga
    49021 tatatgcgaa tgatgtaatg tatgcgtcgt tacaggtttc ttaatagaac taatctcagt
    49081 tgtctctttg atagtgcccc ctttaataat gttgctaatt ttgttcgagt cgatagggcc
    49141 accagctgca tttgtgaata tgtaccctat attaataaac ttatcattac aagcataagt
    49201 gttcttagta agtcgatgct atggatagtg aggcctatag ccctatagct gttacttgtt
    49261 ctagttgtct cttttactcc ggatgctccc gtcatttttc agttatccaa ttaactttac
    49321 cgtcgatatc tagcgtttta tcttcatagt ttatatttac tctctttatt gcaagtagct
    49381 caccgatacg catgtcatta gcaatttgaa actgtaccat agcttttacc attttataat
    49441 tacgttttgt cgttggatat ttttatactt aattacatag tcgaaacaat ccagtaactc
    49501 cttaacttca ttatcttcta atgtgttact atgtttagct agtaacgcat cactataggg
    49561 atatctattt tatctattac acatataact ttgaattgct tgctatttta aattaacaaa
    49621 ttttattctc ttagattttg tccaattatg tgtagacgat ttatagttat taaattcaga
    49681 gtggtagcaa attaaagtta atcaagagtt aagatgaatt taattcatga acacgtctat
    49741 tatttttata attgtagcaa ataaagcttt acatcaagga ggtaattaaa tatgttcaaa
    49801 aaatatgact caaaaaattc aatcgtatta aaatctattc tatcgctagg tatcatctat
    49861 gggggaacat ttggaatata tccaaaagca gacgcgtcaa cacaaaattc ctcaagtgta
    49921 caagataaac aattacaaaa agttgaagaa gtaccaaata attcagaaaa agctttggtt
    49981 aaaaaacttt acgatagata cagcaaggat acaataaatg gaaaatctaa taaatctagg
    50041 aattgggttt attcagagag acctttaaat gaaaaccaag ttcgtataca tttagaagga
    50101 acatacacag ttgctgatag agtatataca cctaagagaa atattactct taataaagaa
    50161 gttgtcactt taaaggaatt ggatcatatc ataagatttg ctcatatttc ttatggctta
    50221 tatatgggag aacatttgcc taaaggtaac atcgtcataa atacaaaaga tggcggtaaa
    50281 tatacattag agtcgcataa agagctacaa aaagataggg aaaatgtaaa aattaataca
    50341 gccgatataa aaaatgtaac tttcaaactt gtgaaaagtg ttaatgacat tgaacaagtt
    50401 tgaaattaag ttgaattagt atatacagtg ttttattgct aatactttga aagttaggta
    50461 tctaaaggtg cctagctttc tttgttatga ttagcactat catatagaaa attctttaat
    50521 gacgtttatg ccgaaatcta caaatataat ttctctttta tggttaatca atatgttttt
    50581 acattcgaca accttaaaat taacttcttt caattccata ataaagtctc tataaaataa
    50641 cttagtttaa aaacgattcg tatctttcag gttcaaatac catcattttc tcctaatact
    50701 tacactttaa ttacaattat gtaagttgtt ttcagaggca tcctatttta tattttgtca
    50761 gaggtgatct tttgaaaacg attttaaaaa caataacata tcttgcactt actatcattg
    50821 gcgcttatgc tgctttattc attttaaaaa caatagactc tcatggtata acagatcaat
    50881 ttaacccatt agtaaaggaa gatgattctt atgttaaaac gacagaggtg tctactagaa
    50941 tggatgatca actccgaagt tatactcaaa gtgcttttaa taaagaaggg aaagagacgc
    51001 aattaatgta tactgctaca tttgatgtta aaccgcatag atacttgaaa attacacata
    51061 aaggtcatca tgtagaaact tttgaagaag ttgaaaagga agaagtacca aagaaagcat
    51121 tagacaaact gagtcgataa tagcatgctt atattacatg gttcatttat aaaaggagta
    51181 cgaacgaaag taacgcatga cgattaattt aaaaatattt gtaataatta tgaataaaat
    51241 taaaaacaag gggtaataca atctatatag catataagct tttgttatga gtttcaaaaa
    51301 taggaagaga gagtgatatt atgaaattaa aatcattagc agtgttatca atgtcagcgg
    51361 tggtgcttac tgcatgtggc aatgatactc caaaagatga aacaaaatca acagagtcaa
    51421 atactaatca agacactaat acaacaaaag atgttattgc tttaaaagat gttaaaacaa
    51481 gcccagaaga tgctgtgaaa aaagctgaag aaacatacaa aggccaaaag ttgaaaggaa
    51541 tttcatttga aaattctaat ggtgaatggg cttataaagt gacgcaacaa aaatctggtg
    51601 aagagtcaga agtacttgtt gctgataaaa ataaaaaagt gattaacaaa aagactgaaa
    51661 aagaagatac agtgaatgaa aatgataact ttaaatatag cgatgctata gattacaaaa
    51721 aagccattaa agaaggacaa aaagaatttg atggtgatat taaagaatgg tcacttgaaa
    51781 aagatgatgg caaacttgtt tacaatatcg atttgaaaaa aggtaataaa aaacaagaag
    51841 ttactgttga tgctaagaac ggtaaagtat taaagagtga gcaagatcac taaaaagatt
    51901 cgttgcatta acgcttgtaa aatgtcttaa ttcatattaa ttaataaaat accctcacaa
    51961 tacttatgca taagtcctgt ttgacgtgaa aggatatatg ccaaacgtgt tgtgagggtt
    52021 taattgcatc tatttatcca tgcgaatatc gacttcttct aaatgtttct gatattcttt
    52081 aaccttactt tctaaaaaca tttcatatgg tgcatcaaag aagtcagcta aatgcatggc
    52141 atctttaaat ttaggttcat gatgatgatt ctcccattcc caaatttgat gtgcttcata
    52201 tttagtacca tatttctcat ttaattgctg tgctaattcg tcaatttcta aattatgttt
    52261 agttcgtaag ttatataaaa tatgcatatt cattgttcat taacctcgct tcttgattta
    52321 aaattcaaag atgctctaat aatatgtgta tattctagtc ctataacata aaataatcaa
    52381 gctatgaatt cggcagaaaa aataatgtaa atgtatatga tattgccagt acagagactt
    52441 taaataacga tagctacatt gaataaaatt gatattcaat tactactttt aaaaatattt
    52501 ggataaaaat aatttgaatt tgttttagaa ttgtaaataa ggggtactac ttagaataac
    52561 gcaaaataaa tagaaaaagg agactgaaaa ttatgtttgg atttattgga atgttaattg
    52621 tcggtggctt aattggatgg gctgctggtg ctattatggg taaagatatc ccaggtggta
    52681 ttttaggcaa tattatcgca ggtattattg gatcatgggt aggtggcaaa ctattcggac
    52741 aatggggtcc tgaattagga agtatttaca tcttgccagc attaattggt tcaattatct
    52801 taattgcaat cgtaacgtta attttaagag ctatgcgtaa ataataaaaa aattcaattg
    52861 gaaattgaaa taaagatggc attcagttgt catctttttt atttgcgtga aaatggtcaa
    52921 tatgtattat aatatttgaa aatgtagat
//
LOCUS       DANBWH010000002        37423 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.002, whole genome shotgun sequence.
ACCESSION   DANBWH010000002 DANBWH010000000
VERSION     DANBWH010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 37423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37423
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.002"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..934)
                     /locus_tag="PPY92_000056"
     CDS             complement(<1..934)
                     /locus_tag="PPY92_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDJ2624088.1"
                     /translation="MRKKWSTLAFGFLVAAYAHIRIKEKRSVKSYMLEQGIRLSRAKR
                     RFMYKEEAMKALEKMAPQTAGEYEGTNYQFKMPVTVDKHYGSTVYNVNDKQDKHQRVV
                     LYAHGGAWFQDPLKIHFEFIDELAETLNAKVIMPVYPKIPHQDYQATYVLFEKLYHDL
                     LNQVADSKQIVVMGDSAGGQIALSFAQLLKEKHIVQPGHIVLISPVLDATMQHPEIPD
                     YLKKDPMVGVDGSVFLAEQWAGDTPLDNYKVSPINGDLDGLGRITLTVGTKEVLYPDA
                     LNLSQLLSAKGIEHDFIPGYYQFHIYPVFPIPERR"
     gene            complement(1055..1411)
                     /locus_tag="PPY92_000057"
     CDS             complement(1055..1411)
                     /locus_tag="PPY92_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDJ2624089.1"
                     /translation="MVKQLNSVEAFREFIHQYPLAVVHVMRDQCSVCHAVLPQIEDLM
                     QSYPNVPLAVINQSQVEAIAGELNIFTVPVDLIFMNGKEMHRQGRFIDMQRFEHHLKQ
                     MNDSVNNDVDEHEYRK"
     gene            complement(1453..1863)
                     /locus_tag="PPY92_000058"
     CDS             complement(1453..1863)
                     /locus_tag="PPY92_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDJ2624090.1"
                     /translation="MTTEGLLVAEKEIEVNGYDIDAMGVVSNIVYIRWFEDLRTAFIN
                     QHMNYSTMINQGISPILMKTEAEYKVPVTIHDKPVGRIYLVKASKMKWVFQFEIVSAH
                     CVHCIGTQTGGFYRLSDKKITSVPQVFQDILATK"
     gene            complement(2243..4309)
                     /locus_tag="PPY92_000059"
     CDS             complement(2243..4309)
                     /locus_tag="PPY92_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HDJ2624091.1"
                     /translation="MFKKLFGQLQRIGKALMLPVAILPAAGILLAFGNAMHNEQLVEI
                     APWLKNDIIVMISSVMEAAGQVVFDNLPLLFAVGTALGLAGGDGVAALAALVGYLIMN
                     ATMGKVLHITIDDIFSYAKGAKELSQAAKEPAHALVLGIPTLQTGVFGGIIMGALAAW
                     CYNKFYNITLPPFLGFFAGKRFVPIVTSVVAIATGVLLSFAWPPIQDGLNSLSNFLLN
                     KNLTLTTFIFGIIERSLIPFGLHHIFYSPFWFEFGSYTNHAGELVRGDQRIWMAQLKD
                     GVPFTAGAFTTGKYPFMMFGLPAAAFAIYKNARPERKKVVGGLMLSAGLTAFLTGITE
                     PLEFSFLFVAPVLYGIHVLLAGTSFLVMHLLGVKIGMTFSGGFIDYILYGLLNWDRSH
                     ALLVIPVGIVYAIVYYFLFDFAIRKFKLKTPGREDEETEIRNSSVAKLPFDVLDAMGG
                     KENIKHLDACITRLRVEVVDKSKVDVAGIKALGASGVLEVGNNMQAIFGPKSDQIKHD
                     MAKIMSGEITKPSETTVTEEMSDEPVHVEALGTTDIYAPGVGQIIPLSEVPDQVFAGK
                     MMGDGIGFIPEKGEIVAPFDGTVKTIFPTKHAIGLESESGVEVLIHIGIDTVKLNGEG
                     FESLINVDEKVTQAQPLMKVNLAYLKAHAPSIVTPMIITNLENKELVIEDVQDADPGK
                     LIMTVK"
     gene            complement(4610..6349)
                     /locus_tag="PPY92_000060"
     CDS             complement(4610..6349)
                     /locus_tag="PPY92_000060"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HDJ2624092.1"
                     /translation="MAKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQF
                     KFYHVRHEEVASLAAAGYTKLTGKIGVALSIGGPGLIHLLNGMYDAKMDNVPQLILSG
                     QTNSTALGTKAFQETNLQKLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVI
                     CPNDLLTEKIKDTTNKPVDTSRPTVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAK
                     DELREFIEMAKIPVIHSLPAKTILPDDHPYSIGNLGKIGTKTSYQTMQEADLLIMVGT
                     NYPYVDYLPKKNIKAIQIDTNPKNIGHRFNINVGIVGDSKIALHQLTENIKHVAERPF
                     LNKTLERKAVWDKWMEQDKNNNSKPLRPERLMASINKFIKDDAVISADVGTATVWSTR
                     YLNLGVNNKFIISSWLGTMGCGLPGAMASKIAYPNRQAIAIAGDGAFQMVMQDFATAV
                     QYDLPLTVFVLNNKQLAFIKYEQQAAGELEYAVDFSDMDHAKFAEAAGGKGYTIKSAS
                     EVDAIVEEALAQDVPTIVDVYVDPNAAPLPGKIVNEEALGYGKWAFRSITEDKYLDLD
                     QIPPISVAAKRFL"
     gene            complement(6400..7089)
                     /locus_tag="PPY92_000061"
     CDS             complement(6400..7089)
                     /locus_tag="PPY92_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDJ2624093.1"
                     /translation="MNDYVQALLMILLTVVLYYFAKRLQQKYPNPFLNPALIASLGII
                     FVLLIFGISYNGYMKGGSWINHILNATVVCLAYPLYKNREKIKDNVSIIFASVLTGVM
                     LNFMLVFLTLKAFGYSKDVIVTLLPRSITAAVGIEVSHELGGTDTMTVLFIITTGLIG
                     SILGSMLLRFGRFESSIAKGLTYGNASHAFGTAKALEMDIESGAFSSIGMILTAVISS
                     VLIPVLILLFY"
     gene            complement(7082..7477)
                     /gene="cidA"
                     /locus_tag="PPY92_000062"
     CDS             complement(7082..7477)
                     /gene="cidA"
                     /locus_tag="PPY92_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like murein hydrolase modulator CidA"
                     /protein_id="HDJ2624094.1"
                     /translation="MHKVQLIIKLLLQLGIIIVITYIGTEIQKIFHLPLAGSIVGLFL
                     FYLLLQFKIVPLTWVEDGANFLLKTMVFFFIPSVVGIMDVASEITLNYILFFAVIIIG
                     TCIVALSSGYIAEKMSVKHKHRKGVDAYE"
     gene            complement(7690..8568)
                     /locus_tag="PPY92_000063"
     CDS             complement(7690..8568)
                     /locus_tag="PPY92_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDJ2624095.1"
                     /translation="MDIKHMKYFIEVVKQGGMTNASKSLYIAQPTISKAIKDIENEMG
                     TPLFDRSKRHLILTDAGQIFYEKSKEIVALYDYLPSEMERLNGLETGHINMGMSAVMN
                     MKILINILGAFHQQYPNVTYNLIENGGKTIEQQIINDEVDIGVTTLPVDHHIFDYTTL
                     DKEDLRLIVSREHRLAKYETVKLEDLAGEDFILFNKDFYLNDKIIENAKNVGFVPNTV
                     AQISQWHVIEDLVTNELGISILPTSISEQLNGDVKLLRIEDAHVHWELGVVWKKDKQL
                     SHATTKWIEFLKDRLG"
     gene            8735..8953
                     /locus_tag="PPY92_000064"
     CDS             8735..8953
                     /locus_tag="PPY92_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HDJ2624096.1"
                     /translation="MSFYEFMQSFLGDDTPLGELVDWINQDSNFPREVKSHSEILSYF
                     RTNPCPESISVPVIKRALSVFNQFTNVQ"
     gene            9343..9774
                     /locus_tag="PPY92_000065"
     CDS             9343..9774
                     /locus_tag="PPY92_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDJ2624097.1"
                     /translation="MEYKKILIRLLIAFAVLFSADFTYQSVEQTHQSHAAVNYYSKNQ
                     CTWWAFKRRAQVGKPVSNRWGNAKNWYYNARKSKYATGRTPRKFAVMQSTAGYYGHVA
                     VVEQVYKNGSIKVSEYNFYRPLKYNTRVLSKKAARNFNYIY"
     gene            complement(9913..11190)
                     /locus_tag="PPY92_000066"
     CDS             complement(9913..11190)
                     /locus_tag="PPY92_000066"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HDJ2624098.1"
                     /translation="MQSLDKNFRHLSRQQKLQQLVDKQWLSEDQFDILLNHPLIDEEV
                     ANSLIENVIAQGALPVGLLPNIIVDDKAYVVPMMVEEPSVVAAASYGAKLVNQTGGFK
                     TVSSERIMIGQIVFDGVDDTEKLSADIKALEKQIHKIADEAYPSIKARGGGYQRIAID
                     TFPEQQLLSLKVFVDTKDAMGANMLNTILEAITAFLKNESPQSDILMSILSNHATASV
                     VKVQGEIDVKDLARGERTGEEVAKRMERASVLAQVDIHRAATHNKGVMNGIHAVVLAT
                     GNDTRGAEASAHAYASRDGQYRGIATWRYDQKRQRLIGTIEVPMTLAIVGGGTKVLPI
                     AKASLELLNVDSAQELGHVVAAVGLAQNFAACRALVSEGIQQGHMSLQYKSLAIVVGA
                     KGDEIAQVAEALKQEPRANTQVAERILQEIRQQ"
     gene            11445..12611
                     /locus_tag="PPY92_000067"
     CDS             11445..12611
                     /locus_tag="PPY92_000067"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HDJ2624099.1"
                     /translation="MTIGIDKINFYVPKYYVDMAKLAEARQVDPNKFLIGIGQTEMAV
                     SPVNQDIVSMGANAAKDIITDEDKKKIGMVIVATESAVDAAKAAAVQIHNLLGIQPFA
                     RCFEMKEACYAATPAIQLAKDYLATRPNEKVLVIATDTARYGLNSGGEPTQGAGAVAM
                     VIAHNPSILALNEDAVAYTEDVYDFWRPTGHKYPLVDGALSKDAYIRSFQQSWNEYAK
                     RQGKSLADFASLCFHVPFTKMGKKALESIIDNADETTQERLRSGYEDAVDYNRYVGNI
                     YTGSLYLSLISLLENRDLQAGETIGLFSYGSGSVGEFYSATLVEGYKDHLDQAAHKAL
                     LNNRTEVSVDAYETFFKRFDDVEFDEEQDAVHEDRHIFYLSNIENNVREYHRPE"
     gene            complement(12776..13297)
                     /locus_tag="PPY92_000068"
     CDS             complement(12776..13297)
                     /locus_tag="PPY92_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDJ2624100.1"
                     /translation="MEYKSYYDSPVGRLELLSDGVSLTAVLFENQQGDGTREENTSLA
                     IFREATQWLDAYFKGDNPEITIPLKPTGSHFQQCVWNELRQVPYGTLTTYGAIAKKVG
                     KVLDKPKMSAQAVGGAVGSNPLSIIVPCHRVVGKTGSLTGFGGTINNKIKLLELENID
                     MSKLYIPKHSTKP"
     gene            13706..15811
                     /gene="clpL"
                     /locus_tag="PPY92_000069"
     CDS             13706..15811
                     /gene="clpL"
                     /locus_tag="PPY92_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpL"
                     /protein_id="HDJ2624101.1"
                     /translation="MNNGFFNSDFDSIFRRMMQDMQGSNQVGNKKYYINGKEVSPEEL
                     AQLTQQGSNQSAEQSAQAFQQAAQRQQGQQGGNGNYLEQIGRNLTQEARDGLLDPVIG
                     RDKEIQETAEVLSRRTKNNPILVGEAGVGKTAIVEGLAQAIVEGNVPAAIKDKEIISV
                     DISSLEAGTQYRGAFEENIQKLIEGVKSSQNAVLFFDEIHQIIGSGATGSDSGSKGLS
                     DILKPALSRGEISIIGATTQDEYRNNILKDAALTRRFNEVLVNEPSAKDTVEILKGIR
                     EKFEEHHQVKLPDDVLKACVDLSIQYIPQRLLPDKAIDVLDITAAHLSAQSPAVDKVE
                     TEKRISELENDKRKAVSAEEYKKADDIQNEIKSLQDKLENSNGEHTAVATVHDISDTI
                     QRLTGIPVSQMDDNDIERLKNISNRLRSKIIGQDQAVEMVSRAIRRNRAGFDDGNRPI
                     GSFLFVGPTGVGKTELAKQLAIDLFGNKDALIRLDMSEYSDTTAVSKMIGTTAGYVGY
                     DDNSNTLTEKVRRNPYSVILFDEIEKANPQILTLLLQVMDDGNLTDGQGNVINFKNTI
                     IICTSNAGFGNGNDAEEKDIMHEMKKFFRPEFLNRFNGIVEFLHLDKDALQDIVNLLL
                     DDVQVTLDKKGITMDVSQDAKDWLIEEGYDEELGARPLRRIVEQQVRDKITDYYLDHT
                     DVKHVDIDVEDNELVVKGK"
     gene            complement(15880..16050)
                     /locus_tag="PPY92_000070"
     CDS             complement(15880..16050)
                     /locus_tag="PPY92_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDJ2624102.1"
                     /translation="MSVIINILIFLATFGYALYTLVKFFKRSKQGKCGTCDINRDCCG
                     IEQHTANHFPGK"
     gene            complement(16063..18057)
                     /gene="feoB"
                     /locus_tag="PPY92_000071"
     CDS             complement(16063..18057)
                     /gene="feoB"
                     /locus_tag="PPY92_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDJ2624103.1"
                     /translation="MENYCILGNPNVGKTSLFNALTGSYEYIGNWSGVTVEKKVGKLK
                     ENVGQLIDLPGTYDLSPISKDETVVTDYLLNDSFSGIINIVDASQLKRNVQLTVQLLE
                     LNQPIYIGLNMIDVATKRGIKIDYHKLMKKLKTPIFPVVARTGKGTKYLLGEIKHLGE
                     GYQPHFKINYGEKIEETIKNMCQIIMTETSHDKYQARFIAIQFLLNNMQIANELNSEV
                     VNKLSSLRDQVAEQVGAVSVRREMERIRNHYIETLLQDVVTYPDEDKQYFSSRIDKIL
                     THKYIGMPIFLAIMWLIFQTTFTWIGTPLSDQLDAFIGGTFTDSVKTIMNYLGVIPFL
                     QDLITDGIIAGVGSVLVFVPQIVVLFFFISLLEDSGYMARIAVLMDRIMESFGLSGKS
                     FIPMIIGFGCNVPSIMAARSIENEKERLTTILIAPFMSCSARLPVYALFVGIFFKENQ
                     SLVVLSLYVLGIIMAFLVSTVLTKTILKNDNAIFIVELPTYRVPSIKTLWRSTWEKAK
                     GFVRKAGTFIFGGSVVIWLLSYVGPHGINVNINQSFLHMVGSFFGMLVQPLGFGTWQA
                     GATLVPGFLAKEVIVSSMAIIYSSGDAGLVNVIQNQFTPLSAYAFMIFILLYIPCVST
                     VAAIRKETYSWKWTALAVAYPLVTAYVLTFIFYQVGHLFV"
     gene            complement(18069..18296)
                     /locus_tag="PPY92_000072"
     CDS             complement(18069..18296)
                     /locus_tag="PPY92_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDJ2624104.1"
                     /translation="MLNIKNGEMNKAYKIKRMDIANENMLYRLSAFGLTDDAIITIKQ
                     KCLFKGPCIIEVNGQQLSIRHCDACSIALEE"
     gene            complement(18391..21000)
                     /gene="farE"
                     /locus_tag="PPY92_000073"
     CDS             complement(18391..21000)
                     /gene="farE"
                     /locus_tag="PPY92_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux MMPL transporter FarE"
                     /protein_id="HDJ2624105.1"
                     /translation="MAKFLYKMGTFIAKHKWSAVIAWIVIVAAILIPLATNAPKFDND
                     IKMTGLESLDTNKKIEKHFNQDSEKAQIRVVFKTTKDDGIVQPNITEDIKKTLEDIKK
                     DDKHIDKISDPYENKQISKDKTTAFADITYDVSQTSLKDGSRDNVKSHLKDLRDNHNV
                     QTELTGTGMTSTEVGGNSELVGIIVAFVVLLITFGSVIAAGLPIISALIGLASGVGII
                     SLLTYAFDIPNVTLTLAVMIGLAVGIDYALFILFRYRQVMKTETDYIKGIGLAIGTAG
                     SAVVFAGVTVVIAVCGLSLVGIDFLAVMGFASAISVIFAVFSALTLLPALISIFHKRI
                     KVNKLQSNFKKDIDTPWSKFITGNALAAVLLGLIILVAAAIPVSHMRLGIPDDGVKPA
                     DSTQKKAYDIISDKFGEGFNGQIPMLINVKDKKDDPQGLQQDLQSVYKDIKDKKNVDI
                     VTPPQMSKDNDYALMVVIPKQGPNAESTNDLVHDLRDYHKDAQDKYGFKTEISGQSVI
                     NIDMSKKLNEAIPLFATVIVVLAFFLLMIVFRSILIPLKAVLGFVLSLMATLGFTTLV
                     MQDGFMKGLFGIETTGPMLAFLPVITIGILFGLAMDYEVFLMSRIHEEYSKTGDNDYS
                     IKVGLKESGPVIVAAALIMFSVFFAFVFQEDVMIKSMGMALAFGVLFDAFVVRMMLIP
                     ALTKLFGKGSWYLPAWLNRIIPRVDIEGHALEKYKTVESQESEAKDSKETYDTTFKVY
                     PQGATNVSKHQDVHGQDDAHSIVLDDKTMALYQEVKQQSASSLFLYDALIDYQNKHQL
                     NSKQQVTNIEQLNKNIEKLNQLLEKIYEINHKITCTCLRFKCLSMTFEAEVFCMEVII
                     QNIFAMSQILHEK"
     gene            21145..21696
                     /gene="farR"
                     /locus_tag="PPY92_000074"
     CDS             21145..21696
                     /gene="farR"
                     /locus_tag="PPY92_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux pump transcriptional regulator
                     FarR"
                     /protein_id="HDJ2624106.1"
                     /translation="MKETDLRVIKTKKALSSSLLQLLEQQLFQTITVNQICDNALVHR
                     TTFYKHFYDKYDLLEYLFNQLTKDYFARDISDRLNHPFQTMSDTINNKEEDLREIAEF
                     QEEDAEFNKVLKNVCIKIMHNDIKNNRDRIDIDSDIPDNLIFYIYDSLIEGFIHWIKD
                     EKIDWPGEDIDNIFHRLINIKIK"
     gene            complement(21773..21940)
                     /locus_tag="PPY92_000075"
     CDS             complement(21773..21940)
                     /locus_tag="PPY92_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624107.1"
                     /translation="MRYVISIIMGIVLAIWSYKQLSQSHLDSGFIFFFIVYVLCISYF
                     NNDKHDKNKKR"
     gene            complement(22052..23596)
                     /gene="pruA"
                     /locus_tag="PPY92_000076"
     CDS             complement(22052..23596)
                     /gene="pruA"
                     /locus_tag="PPY92_000076"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HDJ2624108.1"
                     /translation="MVVEFKNEPGYDFSVQENVDMFKKALKDVEKELGQDIPLVINGE
                     KIFKDDKIKSINPADTSQVIANASKATKQDVEDAFKAANEAYKSWKTWSANDRAELML
                     RVSAIIRRRKAEIAAIMVYEAGKPWDEAVGDAAEGIDFIEYYARSMMDLAQGKPVLDR
                     EGEHNKYFYKSIGTGVTIPPWNFPFAIMAGTTLAPVVAGNTVLLKPAEDTPYIAYKLM
                     EILEEAGLPKGVVNFVPGDPKEIGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVQEG
                     QNFLKRVIAEMGGKDAIVVDENIDTDMAAEAIVTSAFGFSGQKCSACSRAIVHKDVYD
                     EVLEKSIKLTKELTLGNTVDNTYMGPVINKKQFDKIKNYIEIGKEEGKLEQGGGTDDS
                     KGYFVEPTIISGLKSKDRIMQEEIFGPVVGFVKVNDFDEAIEVANDTDYGLTGAVITN
                     NREHWIKAVNEFDVGNLYLNRGCTSAVVGYHPFGGFKMSGTDAKTGSPDYLLHFLEQK
                     VVSEMF"
     gene            complement(23786..24385)
                     /locus_tag="PPY92_000077"
     CDS             complement(23786..24385)
                     /locus_tag="PPY92_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDJ2624109.1"
                     /translation="MTEKEKMLAEKWYDANFDQDLINERARAKDICFELNHTKPSDTN
                     KRKELIDQLFQTTTDNVSISIPFDTDYGWNVKLGKNVYVNTNCYFMDGGQITIGDNVF
                     IGPNCGFYTATHPLNFYHRNEGYEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAG
                     SVVTKDIPPHSLAVGNPCKVVRKIDNDLPSETLNDETIK"
     gene            24624..24815
                     /gene="cwrA"
                     /locus_tag="PPY92_000078"
     CDS             24624..24815
                     /gene="cwrA"
                     /locus_tag="PPY92_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall inhibition responsive protein CwrA"
                     /protein_id="HDJ2624110.1"
                     /translation="MRILITGTVAILIILGLVKTIQDYEMTNDTSRQLSDNKDDDKVI
                     HLNNFKNLHAKEFNPSDFF"
     gene            25055..27463
                     /locus_tag="PPY92_000079"
     CDS             25055..27463
                     /locus_tag="PPY92_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDJ2624111.1"
                     /translation="MANTKKTTLDITGMTCAACSNRIEKKLNKLDDVNAQVNLTTEKA
                     TVEYNPDQHDVQEFINTIQHLGYGVTVETVELDITGMTCAACSSRIEKVLNKMNGVQN
                     ATVNLTTEQAKVDYYPEETDADKLVTRIQKLGYDASIKDNNKDQTSRKAEALQHKLIK
                     LIISAVLSLPLLMLMFVHLFNMHIPALFTNPWFQFILATPVQFIIGWQFYVGAYKNLR
                     NGGANMDVLVAVGTSAAYFYSIYEMVRWLNGSTTQPHLYFETSAVLLTLILFGKYLEA
                     RAKSQTTNALGELLSLQAKEARILKDGNEVMIPLNEVHVGDTLIVKPGEKIPVDGKII
                     KGMTAIDESMLTGESIPVEKNVDDTVIGSTMNKNGTITMTATKVGGDTALANIIKVVE
                     EAQSSKAPIQRLADIISGYFVPIVVGIALLIFIVWITLVTPGTFEPALVASISVLVIA
                     CPCALGLATPTSIMVGTGRAAENGILFKGGEFVERTHQIDTIVLDKTGTITNGRPVVT
                     DYHGDNQTLQLLATAEKDSEHPLAEAIVNYAKEKQLTLTETTTFKAVPGHGIEATIDH
                     HHILVGNRKLMADNDISLPKHISDDLTHYERDGKTAMLIAVNYSLTGIIAVADTVKDH
                     AKDAIKQLHDMGIEVAMLTGDNKNTAQAIAKQVGIDTVIADILPEEKAAQIAKLQQQG
                     KKVAMVGDGVNDAPALVKADIGIAIGTGTEVAIEAADITILGGDLMLIPKAIYASKAT
                     IRNIRQNLFWAFGYNIAGIPIAALGLLAPWVAGAAMALSSVSVVTNALRLKKMRLEPR
                     RKDA"
     gene            28001..28207
                     /gene="copZ"
                     /locus_tag="PPY92_000080"
     CDS             28001..28207
                     /gene="copZ"
                     /locus_tag="PPY92_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HDJ2624112.1"
                     /translation="MSQEILNVEGMSCGHCKSAVESALNNIDGVTSADVNLENGQVSV
                     QYDDSKVAVSQMKDAIEDQGYDVV"
     gene            complement(28298..29296)
                     /locus_tag="PPY92_000081"
     CDS             complement(28298..29296)
                     /locus_tag="PPY92_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDJ2624113.1"
                     /translation="MTKIKIMSVRDEDMPYIKAWAEKHHVEVDITKEALTDDNVEGVA
                     GYDGLSLSQQIPLSEHVYKRLNELGIKQIAQRSAGFDTYDLELANKYNLIVSNVPSYS
                     PNSIAEFAVNQAINVVRHFNQIQTKVREHDFRWEPTILSKSIKDLKVAVIGTGRIGRV
                     VADIFANGYQSDVVAYDPFPNAKIATYVDYKDTIEEAVEGADIVTLHVPATKYNHYLF
                     NAELFKHFKKGAVFVNCARGSLVDTKALLDALDNGVIKGAALDTYEFERKLFPSDQRG
                     KTPNDPLLESLIDREDVILTPHIAFYTEAAVKNLIVDALDATLDVLQTGDTRLRVN"
     gene            complement(29319..30473)
                     /locus_tag="PPY92_000082"
     CDS             complement(29319..30473)
                     /locus_tag="PPY92_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDJ2624114.1"
                     /translation="MISNKLANIPDSYFGKTMGRKIEHGPLPLINMAVGIPDGPTPQG
                     IIDHFQKALTIPENQKYGAFHGKEAFKQAIVDFYQRQYNVTLDKEDEVCILYGTKNGL
                     VAVPTCVINPGDYVLLPDPGYTDYLAGVLLADGKPVPLNLEPPHYLPDWSTVDSQIID
                     KTKLIYLTYPNNPTGSTATKEVFDEAIAKFKGTDTKIVHDFAYGAFGFDAKNPSILAS
                     ENGKDVAIEIYSLSKGYNMSGFRVGFAVGNKDMIQALKKYQTHTNAGMFGALQDAAIY
                     ALNHYDDFLEEQSNVFKTRRDRFEAMLAKADLPFVHAKGGIYVWLETPPGYDSEQFEQ
                     FLVQEKSILVAPGKPFGENGNRYVRISLALDDQKLDEAAIRLTELAYLYE"
     gene            complement(30901..32409)
                     /locus_tag="PPY92_000083"
     CDS             complement(30901..32409)
                     /locus_tag="PPY92_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDJ2624115.1"
                     /translation="MKIAVIGAGVTGLAAAARIASQGHEVTIFEKNNNVGGRMNQLKK
                     DGFTFDMGPTIVMMPDVYKDVFTACGKNYEDYIELRQLRYIYDVYFDHDDRITVPTDL
                     AELQQMLESIEPGSTHGFMSFLTDVYKKYEIARRYFLERTYRKPSDFYNMTSLVQGAK
                     LKTLNHADQLIEHYIDNEKIQKLLAFQTLYIGIDPKRGPSLYSIIPMIEMMFGVHFIK
                     GGMYGMAQGLAQLNKDLGVNIELNAEIEQIIIDPKFKRADAIKVNGDIRKFDKILCTA
                     DFPSVAESLMPDFAPIKKYPPHKIADLDYSCSAFLMYIGIDIDVTDQVRLHNVIFSDD
                     FRGNIEEIFEGRLSYDPSIYVYVPAVADKSLAPEGKTGIYVLMPTPELKTGSGIDWSD
                     EALTQQIKEIIYRKLATIEVFEDIKSHIVSETIFTPNDFEQTYHAKFGSAFGLMPTLA
                     QSNYYRPQNVSRDYKDLYFAGASTHPGAGVPIVLTSAKITVDEMIKDIERGV"
     gene            complement(32421..33284)
                     /locus_tag="PPY92_000084"
     CDS             complement(32421..33284)
                     /locus_tag="PPY92_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HDJ2624116.1"
                     /translation="MTMMDMNFKYCHKIMKKHSKSFSYAFDLLPEDQRKAVWAIYAVC
                     RKIDDSIDVYGDIQFLNQIKEDIQSIEKYPYEHHHFQSDRRIMMALQHVAQHKNIAFQ
                     SFYNLIDTVYKDQHFTMFETDAELFGYCYGVAGTVGEVLTPILSDHETHQTYDVARRL
                     GESLQLINILRDVGEDFDNERVYFSKQRLKQYEVDIAEVYQNGVNNHYIDLWEYYAAI
                     AEKDFQDVMDQIKVFSIEAQPIIELAARIYIEILDEVRQANYTLHERVFVDKRKKAKL
                     FHEINSKYHRI"
     gene            complement(33321..34448)
                     /gene="crtQ"
                     /locus_tag="PPY92_000085"
     CDS             complement(33321..34448)
                     /gene="crtQ"
                     /locus_tag="PPY92_000085"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,4'-diaponeurosporenoate glycosyltransferase"
                     /protein_id="HDJ2624117.1"
                     /translation="MKWLSRILTVIVTMSMACGALIFNRRHQLKTKTLNFNHKALTII
                     IPARNEEKRIGHLLHSIIQQQVPVDVIVMNDGSTDETARVARSYGATVVDVVDDTDGK
                     WYGKSHACYQGVTHACTNRIAFVDADVTFLRKDAVETLINQYQLQGEKGLLSVQPYHI
                     TKRFYEGFSAIFNLMTVVGMNVFSTLDDGRTNQHAFGPVTLTNKEDYYATGGHKSANR
                     HIIEGFALGSAYTSQSLPVTVYEGFPFVAFRMYQEGFQSLQEGWTKHLSTGAGGTKPK
                     IMTAIVLWLFGSIASILGLCLSLKYRQMSVRKMVALYLSYTTQFIYLHRRVGQFSNLL
                     MVCHPLLFMFFTKIFIQSWKQTHRYGVVEWKGRQYSISKEQ"
     gene            complement(34454..35947)
                     /locus_tag="PPY92_000086"
     CDS             complement(34454..35947)
                     /locus_tag="PPY92_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDJ2624118.1"
                     /translation="MTKHIIVIGGGLGGISAAIRMAQSGYSVSLYEQNTHIGGKVNRH
                     ESDGFGFDLGPSILTMPYIFEKLFEYSKKQMSDYVTIKRLPHQWRSFFPDGTTIDLYE
                     GIKETGQHNAILSKQDIEELQNYLNYTRRIDRITEKGYFNYGLDTLSQIIKFHGPLNA
                     LINYDYVHTMQQAIDKRISNPYLRQMLGYFIKYVGSSSYDAPAVLSMLFHMQQEQGLW
                     YVEGGIHHLANALEKLAREEGVTIHTGARVDNIKTYQRRVTGVRLDTGEFVKADYIIS
                     NMEVIPTYKYLIHLDTQRLNKLEREFEPASSGYVMHLGVACQYPQLAHHNFFFTENAY
                     LNYQQVFHEKVLPDDPTIYLVNTNKTDHTQAPVGYENIKVLPHIPYIQDQPFTTEDYA
                     KFRDKILDKLEKMGLTDLRKHIIYEDVWTPEDIEKNYRSNRGAIYGVVADKKKNKGFK
                     FPKESQYFENLYFVGGSVNPGGGMPMVTLSGQQVADKINAREAKNRK"
     gene            complement(35940..36428)
                     /gene="crtO"
                     /locus_tag="PPY92_000087"
     CDS             complement(35940..36428)
                     /gene="crtO"
                     /locus_tag="PPY92_000087"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl-4,4'-diaponeurosporenoate
                     acyltransferase"
                     /protein_id="HDJ2624119.1"
                     /translation="MKKYIKTAFFCSMYWLIVQLNIANLGTRIPDKYFRQKYIIFKSF
                     NFEKHGKFWNKWFYVRKWKHKILDGHQLNQNIYDQRHLMTINTDEIEKMIIETKRAEL
                     IHWISILPVIIFNKGSRLVKYINIFYAMIANVPIIIVQRYNRPRLTQLLRILKRRGER
                     HD"
     gene            complement(36603..37370)
                     /locus_tag="PPY92_000088"
     CDS             complement(36603..37370)
                     /locus_tag="PPY92_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDJ2624120.1"
                     /translation="MKKIVTATIATAGLATIAFAGHDAQAAEQNNNGYNSNDAQSYSY
                     TYTIDAQGNYHYTWTGNWNPSQLTQNNTYYYNNYNTYSYNNASYNNYYNHSYQYNNYT
                     NNSQTATNNYYTGGSGASYSTTSNNVHVTTTAAPSSNGRSISNGYASGSNLYTSGQCT
                     YYVFDRVGGKIGSTWGNASNWANAAASSGYTVNNTPKVGAIMQTTQGYYGHVAYVEGV
                     NSNGSVRVSEMNYGHGAGVVTSRTISANQAGSYNFIH"
BASE COUNT        11911 a         7205 c         5780 g        12527 t
ORIGIN      
        1 gtcggcgctc tggtatcgga aatactggat aaatatggaa ttggtaatat ccaggtataa
       61 agtcatgttc gatacctttt gcactcaaca attgcgataa gttcaaagca tctgggtaca
      121 gtacttcttt tgtgccaaca gttaatgtaa tacgacctaa accatctaag tcaccattaa
      181 ttggtgatac tttgtagtta tctaaaggtg tgtcccctgc ccattgttca gctaagaaca
      241 cactgccatc cacacctacc attgggtctt tctttaagta gtcaggaatt tcaggatgct
      301 gcatcgttgc atctaaaact ggtgaaatta atacaatatg tcctggttgc acaatatgtt
      361 tttcttttaa caattgagca aatgataaag caatttgacc gcccgcagag tcacccatta
      421 caacgatttg tttagaatct gctacttgat tcaataaatc atggtataac ttttcaaaaa
      481 gcacatacgt cgcttgataa tcttgatgcg gaatcttcgg atatactggc atgatgactt
      541 tagcattgag tgtttctgca agttcatcaa taaattcgaa atgaattttg agtgggtctt
      601 ggaaccatgc gcctccatgt gcgtataata caacgcgttg atgcttatct tgtttatcat
      661 taacgttata aacggttgaa ccgtagtgct tatccactgt tactggcatc ttaaactgat
      721 aattggtgcc ctcatattca cctgctgtct gtggcgccat tttttctaat gctttcatcg
      781 cttcttcttt atacataaaa cgacgcttag ctctagataa tcgtatacct tgttctaaca
      841 tataactttt cacactgcgt ttttctttaa ttctaatatg tgcgtatgct gctactaaaa
      901 atccaaacgc aagtgtagac cattttttac gcatttgtcg tcactcctct ttgatgattt
      961 ctactatagt tctactgtac catgctgatt cttattacta ccaatattta ctgggaaata
     1021 agttatgatg tctacataat cttagcaatg ctaatcattt gcgatattca tgctcatcga
     1081 catcgttatt cacactatca ttcatttgct taagatgatg ttcaaaacgt tgcatatcga
     1141 taaaacgccc ttgacgatgc atttcttttc cattcataaa aattaaatcc acaggtacag
     1201 tgaaaatatt taattctcca gcaatagctt caacttgact ttgattaatc acagctaatg
     1261 gcacattggg atatgattgc atcaagtctt caatttgtgg taaaacggca tgacacacgc
     1321 tacactgatc gcgcatgaca tgtacaactg ctaacggata ttgatgaata aattcacgga
     1381 atgcttcgac actatttaat tgcttaacca tttcgaacac cttcttactt tttatatttt
     1441 aaaatgaagt cattattttg ttgctaaaat gtcttgaaac acttgtggca cagaggttat
     1501 cttcttatca ctcaatctgt aaaaaccgcc tgtctgtgta ccaatacaat gcacgcaatg
     1561 tgcggacaca atttcaaact gaaacaccca tttcatcttg cttgctttaa ctaagtaaat
     1621 acgacctact ggtttgtcat gtattgtgac aggtacttta tactctgctt ccgttttcat
     1681 aagtataggt gaaatgcctt gattgatcat tgttgagtaa ttcatgtgct gattaataaa
     1741 cgctgttctc aaatcttcga accatctaat ataaacgata ttactaacga cacccatcgc
     1801 atcaatgtcg taaccattca cttcgatttc tttctctgca actaatagac cttctgttgt
     1861 cataatgaca aatctcctta tcttacaaat agatttaaaa tcatgttaat ttatttttat
     1921 tactttcatg ataaaaatac aagcaattga actttattac ggaaaatcaa acttttcaat
     1981 gagactatta atttggctag tcgcgaagat gcatttttct aaaatgtgac aaaaacataa
     2041 gcaatgtttt ctatatctaa cactaatatt taacttatta tcatcaacac acctgctata
     2101 catcattgcg cactaaaaag gcagtagctt cattttaagc tactgccttt tgtaacgtat
     2161 tctctttcaa ctcgctactt cgcatacgca ctacgaatga ctaaaagttc attcatttga
     2221 tatgctgttt catttttaat cattatttga ctgtcataat tagcttacct ggatcagcat
     2281 cttgtacatc ttcaatgaca agttctttat tttcaagatt tgtaataatc attggtgtaa
     2341 cgatgcttgg tgcgtgtgct ttcaagtatg ctaaattcac tttcattaat ggttgggctt
     2401 gtgttacttt ttcatcaacg ttaatcagac tttcgaatcc ttcaccattc agtttcactg
     2461 tatcgatacc aatatgaata agtacttcga cgccactttc agattctaat cctatcgcat
     2521 gtttcgtagg gaagattgtt ttcactgtac catcaaacgg tgctacaatt tcaccttttt
     2581 cagggataaa gccaatacca tcacccatca ttttaccagc gaatacttga tcaggtactt
     2641 ctgataatgg aatgatttga ccgacacctg gtgcatagat gtctgttgtt ccaagtgctt
     2701 ctacgtgaac tggttcatct gacatttctt cagtcactgt cgtttcactt ggtttcgtaa
     2761 tttcaccact cataatcttg gccatatcat gtttaatttg atctgatttt ggaccaaaga
     2821 tagcttgcat attgtttcca acttctaata cacctgatgc gcctaaagct ttaatacctg
     2881 ctacatctac ttttgattta tcaaccactt ctacgcgcag acgtgtaata catgcatcta
     2941 aatgtttaat gttttctttt ccacccattg catctaagac atcaaatggt aattttgcga
     3001 cactagagtt acgaatttca gtttcttcat cttcacgacc tggtgttttc aatttaaact
     3061 tacgaattgc aaagtcgaat aagaagtaat acacgatagc atatacaata ccgactggaa
     3121 taactaataa tgcgtgtgaa cgatcccagt ttaataaacc atataaaata taatctatga
     3181 aaccacctga gaatgtcata ccaattttaa cgcctaataa atgcattact aagaatgatg
     3241 taccagctaa taatacgtga attccataaa gtactggtgc tacaaataag aatgaaaatt
     3301 ctaatggctc agtgatacca gttaaaaatg cagttaatcc tgctgataac attaaaccac
     3361 ccacgacttt tttgcgttct ggtcgtgcat ttttataaat agcaaatgcc gccgctggta
     3421 aaccaaacat cataaatgga tatttaccag tagtaaatgc accagcagta aatggtacgc
     3481 catctttcaa ttgtgccatc caaatacgtt ggtcaccacg aactaattca cctgcgtgat
     3541 ttgtataact tccgaattca aaccagaacg gtgaatagaa aatatgatgt aaaccaaatg
     3601 gaattaatga gcgttcaata ataccgaata taaacgttgt taatgttaaa tttttattta
     3661 ataagaaatt cgataaacta tttaatccat cttgaattgg tggccacgca aagcttaaaa
     3721 gcacacctgt tgcgattgct acgaccgatg tcacaatagg tacaaatcgt ttacctgcaa
     3781 agaatcctaa aaatggtggt agtgtaatat tataaaattt gttgtaacac catgcggcta
     3841 aagcacccat gataatacca ccaaacacac ccgtttgtaa cgttggaata cctaatacta
     3901 aagcatgtgc tggttctttc gctgcttgac ttaattcttt tgcccctttg gcatatgaga
     3961 aaatgtcatc aattgtaatg tgcaacactt tccccattgt tgcattcata attaagtaac
     4021 ctactagcgc tgctaatgct gcaacaccgt ctcctcctgc taatccaagt gctgtaccaa
     4081 ctgcaaataa taatggcaag ttatcaaata caacttgtcc tgctgcttcc atgaccgacg
     4141 aaatcattac aatgatatcg ttttttaacc atggtgcaat ttctactaat tgttcgttgt
     4201 gcattgcgtt accaaacgct aataaaatac cagctgctgg taaaatcgca acaggtaaca
     4261 ttaatgcttt accgatacgt tgcaattgtc caaacaattt cttaaacata taaacatccc
     4321 ctatctcaat ttccgcttcc atgtacaaaa aaagcaggca tgaacaaaca agactattct
     4381 catagtacgg ttgtttgctc atgcctgcat taccagtaac acgcaagtga tatgtaattc
     4441 atagctatta tacatatttg taaaagcgtt ttcaacaact ttttcacaat tttatcatca
     4501 attcagtacg atcatttgtg ataaataaac aaaacggcaa ttcccatttt gttgaaatag
     4561 aaattaccgt ttttatagtt caattcaatt gtgataacct ttcaatcagt tataagaaac
     4621 gttttgctgc aactgaaata ggtggaattt gatctaaatc taaatattta tcttcagtaa
     4681 tagatctaaa tgcccattta ccataaccaa gcgcttcttc atttacaatt ttacctggta
     4741 atggcgcagc attaggatca acatatacat ctacaatcgt tggtacatct tgtgctaatg
     4801 cctcttcgac tatagcatct acttcgctag cactcttaat tgtataacct ttaccacctg
     4861 ctgcctcagc aaattttgca tgatccatat cagaaaaatc aactgcatat tctaattcac
     4921 cagctgcttg ttgttcatat ttaataaatg ctaactgttt gttattaagt acaaatacag
     4981 ttaaaggtaa atcatattgt actgctgtag cgaagtcttg cattaccatt tggaatgcac
     5041 cgtcaccagc aatggcaatc gcttgtctat ttggatatgc aatttttgat gccatagctc
     5101 ctggaagacc gcaacccatt gtacctaacc aacttgaaat gatgaacttg ttgttcacac
     5161 caagatttaa ataacgtgta gaccaaactg ttgcagtacc aacatctgct gaaattactg
     5221 catcatcttt aataaatttg ttaattgatg ccattaaacg ttctggacgt aatggtttgc
     5281 tattattatt tttatcttgt tccatccatt tatcccaaac cgctttacgt tctaacgttt
     5341 tgtttaaaaa tggtctttcc gcaacatgtt taatattttc agttaactga tgcaatgcaa
     5401 ttttactgtc tccaacaatt cctacattaa tattaaaacg atgtccgata tttttaggat
     5461 ttgtgtcgat ttggattgct ttaatatttt tcttaggtaa gtaatccaca tatggatagt
     5521 ttgtaccaac cataattaat aaatccgctt cctgcattgt ttgataagat gttttggtac
     5581 caattttacc taagttaccg atactatatg gatgatcatc cggcaagatt gttttagctg
     5641 gtaatgaatg aatgacagga attttagcca tttcaataaa ttcacgtagc tcatctttcg
     5701 catgttttgc acctacacca attaacatga caggcttttt acttttatta attagtttaa
     5761 ccgctttttt gatgtcttta tattttggtg atacaactgt tggtcttgat gtatctactg
     5821 gtttattcgt tgtatcttta attttttcag ttaataagtc gttaggacaa ataacaacag
     5881 cgacaccttt ttgttcatat gccgtacgaa ttgcttcgtt aacgatttca aacacattgt
     5941 cacctttttc aatttggtga ttataaacgg ctacatcttc acataatttt tgtaaatttg
     6001 tttcttgaaa tgctttcgtt ccaagtgctg tactattcgt ttgtccagat aatattaatt
     6061 gcggtacatt atccattttg gcatcgtaca taccatttaa taaatgaatt aaaccagggc
     6121 caccgatact taatgccaca ccgattttac cagttaattt tgtgtaacca gcagccgcta
     6181 agcttgctac ttcttcatga cgtacatgat aaaatttaaa ttgatctctc actgtacgta
     6241 aactatcgac aactgcgtcg attgagtctc ctggaatacc atacaagtga tctatatccc
     6301 atgcttgtaa tgctttaact aatgcttcat ttgcttttat ttttgccatt attactaata
     6361 gcctcccttt cttagtgtct atcattttaa atatctaaat taatagaata ataaaattag
     6421 aacaggtatt aacactgaac taataactgc agttaaaatc atcccaattg aactaaatgc
     6481 accggattca atatccattt ctagtgcttt agctgtgcca aatgcatgtg acgcattccc
     6541 atacgttaaa cctttggcga tagaagattc aaatcttcca aatcttaata acatcgaacc
     6601 taaaatacta ccgattaaac ccgttgtgat aataaaaagt acggtcatcg tatctgtacc
     6661 acctagttca tgtgacactt cgatacctac tgcggctgtt atagatcggg gcaataacgt
     6721 tacaatgacg tctttagaat agccaaatgc tttaagtgtt aagaacacta acatgaaatt
     6781 cagcatgacg ccagttaata cacttgcaaa aatgatagag acattgtctt taattttctc
     6841 tctattttta taaagtgggt acgctaaaca tacgaccgtt gcgtttaaaa tatggttgat
     6901 ccaactgcca cctttcatat acccgttata actaattcca aagataagta agacaaaaat
     6961 aattcctaaa gatgcaatta atgctggatt caaaaatggg ttcggatatt tttgttgtaa
     7021 ccttttagcg aaataatata aaacgacagt caacaaaatc attaataagg cttgcacgta
     7081 atcattcata agcgtctaca cctttacgat gtttatgttt aacagacatt ttttcagcaa
     7141 tataacctga agataatgca acgatacatg ttcctataat aatgactgcg aaaaagagta
     7201 tataatttag cgtaatttcg gaagcaacat ccataatacc tacaactgac ggtatgaaga
     7261 aaaagaccat cgtctttaat aaaaagtttg caccgtcttc tacccaagtt agcggtacaa
     7321 tcttaaattg taatagtaaa taaaataaaa atagaccaac aatactgccg gctaagggaa
     7381 gatgaaaaat cttttgaatt tctgtgccaa tataagtaat cacaatgatg attcctagtt
     7441 gtagtagtag tttgattatt aattggactt tgtgcatggc gccatccctt tctaaatatg
     7501 tctaaaatgt tacaataact attataaaga tggcgtgcac aatataaaaa tactcttttt
     7561 ttatagtttg tattactatt ttctatggaa acgctctctc aatttcactt cattttcaca
     7621 aaaaaacact catcatttca atgacccacg taatgatgcg ccttaaataa tgagtgcttt
     7681 attaatatgt tagcctaaac gatctttcaa aaattctatc catttcgttg tggcatgact
     7741 taattgttta tccttcttcc aaacaacacc taattcccaa tgtacatgcg catcttcaat
     7801 gcgtagcagc ttcacatctc catttagttg ctctgaaatt gatgttggta aaatactaat
     7861 acctaattca ttcgtaacta aatcttctat aacatgccat tgtgaaattt gcgctacagt
     7921 attcggaaca aagccaacgt ttttagcatt ttcaataatt ttatcattca agtaaaagtc
     7981 tttattaaat aaaatgaagt cttcacctgc taaatcttcg agtttaacag tttcatattt
     8041 tgcgagtcga tgctctctgc tcacgataag tcgcaaatct tccttatcta gggtagtata
     8101 atcgaaaata tgatgatcga ctggcaaagt ggtcacgcct atatctactt catcattgat
     8161 aatttgctgc tcaatcgttt tgccgccatt ttctattaaa ttatatgtaa catttggata
     8221 ttgctgatgg aatgcaccaa gaatattgat aagaatcttc atattcatga ctgccgacat
     8281 gcccatgttt atatgccctg tttccagtcc attcaagcgt tccatttcag atggtaaata
     8341 atcatacagt gcaacaattt ctttactttt ctcataaaaa atttgacctg catcggtaag
     8401 tattaaatgt cttttacttc tatcaaataa gggcgtgccc atttcatttt caatatcttt
     8461 aattgcttta ctaattgtag gttgtgcaat atataatgat ttggaggcat tagtcatgcc
     8521 tccttgctta acgacttcaa taaaatattt catatgtttg atatccacta acatcatcct
     8581 aactatttca acacgtttat ttcattatac acaacttctt aaacattcaa taaatttaga
     8641 tttataaaat tgctttataa aaagttttat gtaaaataca cattctttta tggtattata
     8701 ttaatggctc aagtttgtta ttcgaggtga ggtattgagc ttttacgaat ttatgcaaag
     8761 ttttcttggt gatgacacac cattaggtga attggttgat tggattaatc aagatagcaa
     8821 tttccctaga gaagtgaaga gccatagtga aatattgtca tattttcgaa caaatccatg
     8881 tcctgaaagc atctcagtac ctgtaattaa acgggcacta tcagttttca accaattcac
     8941 aaatgtacaa tgatacatga atacatggag tcacatcatc tcatgttagc gctctgattg
     9001 cagggcgcta tttttaatgt tatcgctaca tttagtatat tgtgattgct attttctttc
     9061 agttatagaa ccacgtacct tttcttttaa atcattttat attttcccac taatatccgc
     9121 tgttaatcaa ttctgacatc cttgtatcac tatgacaatt aattgttaaa tacatgaatt
     9181 tctacatttt atgaaaaaat ccatttttat tacaattcaa cactttatat gacaacttca
     9241 ttacagttac ttttattgtt gattgcttgc attgttttct aaaaaaattt tgttatcata
     9301 attaacgttg aataaagaaa aaaattaact tgggagataa aaatggaata taaaaagata
     9361 ctaattcgtt tattaattgc ttttgcagta cttttctcag cagatttcac ttatcaatcc
     9421 gtcgaacaaa cgcatcaatc gcatgccgca gttaattatt atagtaaaaa ccaatgtaca
     9481 tggtgggcat ttaaacgtcg cgcacaagtc ggcaaacctg tttctaatag atggggcaat
     9541 gctaaaaatt ggtattacaa tgcacgtaaa tcaaaatatg cgactggtcg tacaccaaga
     9601 aaatttgctg tcatgcaatc aactgcagga tattatggac atgtcgcagt tgttgaacaa
     9661 gtatataaaa acggtagtat taaagtttca gaatacaact tttatcgccc attaaaatac
     9721 aatacacgtg tactaagcaa aaaggcagca cgtaacttta actatattta ctaatcaaaa
     9781 aacttctatc acgaacgctt caatttcctg tatgcgtgtg atagaagttt ttattttttg
     9841 ttcatattaa tttatctaag cgctacgatg gactgactta aacttttctt taaaatcgct
     9901 attcgccatt tactattgtt gtctaatttc ttgtaaaatg cgttcagcta cttgtgtatt
     9961 cgcacggggt tcttgcttca atgcttcagc tacttgcgca atttcatcac cttttgcacc
    10021 tacaacaata gctaaagatt tatattgcaa gctcatatgg ccttgctgga taccttcgga
    10081 aacgagcgcg cgacatgctg caaagttctg tgctaaacca acggcagcaa ctacatgacc
    10141 taattcttgt gctgaatcta catttagcaa ttctaaagaa gctttagcaa ttggtaatac
    10201 ttttgtacca ccgccaacga ttgccaatgt cataggcact tctattgtac caattaaacg
    10261 ttgacgtttt tgatcgtatc tccatgttgc aataccacga tactgtccgt cacgactcgc
    10321 gtatgcatgc gcacttgctt ctgcaccacg cgtatcattt cctgttgcta aaacaacggc
    10381 atgtatgcca ttcataacac ctttattatg tgttgcagca cgatgaatat caacttgtgc
    10441 caatacagaa gcacgttcca ttcgtttggc aacctcttct ccagttctct cgccccttgc
    10501 taaatcttta acgtcaattt cgccttgaac tttaacaacg gacgctgttg catgattgga
    10561 taaaatactc attaaaatgt cgctttgtgg agattcattt tttaaaaatg cagttatggc
    10621 ctctaaaatc gtattaagca tattagcgcc catagcatct ttcgtatcaa caaatacttt
    10681 taaagatagt aactgttgct caggaaatgt atcaatagct atacgttggt aaccaccacc
    10741 acgcgcttta atagaaggat atgcctcatc cgcaatttta tgaatttgct tttctaaagc
    10801 tttaatgtct gctgataatt tttcagtatc gtcaacgcca tcaaagacga tttgacctat
    10861 cataatacgt tcagaagata ccgttttaaa tccgccagtc tgattcacta gctttgcacc
    10921 ataactagct gcagcgacaa ctgaaggctc ttccaccatc ataggtacaa catatgcctt
    10981 atcgtccaca atgatattcg gtaataatcc aacgggtaat gcaccttgcg cgatgacatt
    11041 ttcaattaaa ctatttgcta cttcctcatc aattaatgga tgattcaata aaatgtcgaa
    11101 ttgatcttct gataaccatt gcttatctac caattgttgt aacttttgtt gacgagataa
    11161 atgtcggaaa ttcttatcta aactttgcat ggacatactc cttttacttc acataatttt
    11221 ttaacatttc aatcactact atttttacca taaaataacg ttattcgtct taaaattcaa
    11281 ttgaataatt attgttttaa ctataaaata aagcaaggta aattaaaacg cttacaagaa
    11341 acgtcaaatc atttttaaat ttagtgtatt tctttgtata agataagcat attctgatat
    11401 gatacaagtg ttgcttttat aaatttgaaa ggatgtaaaa ccttatgaca ataggtatcg
    11461 acaaaataaa cttttacgtt ccaaagtact atgtagacat ggctaaatta gcagaagcac
    11521 gccaagtaga cccaaacaaa tttttaattg gaattggtca aactgaaatg gctgttagtc
    11581 ctgtaaacca agacatcgtt tcaatgggcg ctaacgctgc taaggacatt ataacagacg
    11641 aagataaaaa gaaaattggt atggtaattg tggcaactga atcagcagtt gatgctgcta
    11701 aagcagccgc tgttcaaatt cacaacttat taggtattca accttttgca cgttgctttg
    11761 aaatgaaaga agcttgttat gctgcaacac cagcaattca attagctaaa gattatttag
    11821 caactagacc gaatgaaaaa gtattagtta ttgctacaga tacagcacgt tatggattga
    11881 attcaggcgg cgagccaaca caaggtgctg gcgcagttgc gatggttatt gcacataatc
    11941 caagcatttt ggcattaaat gaagatgctg ttgcttacac tgaagacgtt tatgatttct
    12001 ggcgtccaac tggacataaa tatccattag ttgatggtgc attatctaaa gatgcttata
    12061 tccgctcatt ccaacaaagc tggaatgaat acgcaaaacg tcaaggtaag tcgctagctg
    12121 acttcgcatc tctatgcttc catgttccat ttacaaaaat gggtaaaaag gcattagagt
    12181 caatcattga taacgctgat gaaacaactc aagagcgttt acgttcagga tatgaagatg
    12241 ctgtagatta taaccgttat gtcggtaata tttatactgg atcattatat ttaagcctaa
    12301 tatcattact tgaaaatcgt gatttacaag ctggtgaaac aatcggttta ttcagttatg
    12361 gctcaggttc agttggtgaa ttttatagtg cgacattagt tgaaggctac aaagatcatt
    12421 tagatcaagc tgcacataaa gcattattaa ataaccgtac tgaagtatct gttgatgcat
    12481 atgaaacatt cttcaaacgt tttgatgacg ttgaatttga cgaagaacaa gatgctgttc
    12541 atgaagatcg tcatattttc tacttatcaa atattgaaaa taacgttcgc gaatatcaca
    12601 gaccagagta atctgtgtat ttgaaagaca tttaaatata acctaaaaac agcagtaaga
    12661 cagcttctca ttgaaatcgt cttactgctg ttctctattt ataacacttc gtattgaatg
    12721 aattcattat gcctatttga cacattattg aaatttttaa tatacccctt atacgttacg
    12781 gctttgtact gtgtttaggt atataaagct ttgacatatc gatattctcc aactctaaca
    12841 gcttaatttt attattaatc gttccaccga atcctgttaa gctacccgtt ttaccgacaa
    12901 cacgatgaca tggcacgata attgataatg gattacttcc gactgcacct ccaaccgctt
    12961 gggctgacat ttttggctta tcaagcacct tacctacttt tttggcaata gcaccatacg
    13021 ttgttagagt cccataagga acctgtctta attcattcca aacacactgt tgaaaatgac
    13081 tacctgttgg ctttaaaggt attgtgattt caggattgtc acctttaaaa tacgcgtcta
    13141 accactgtgt cgcctctcta aatatcgcta aagacgtatt ttcttcccta gtaccatcac
    13201 cttgttgatt ttcaaacaaa acagcggtca gacttacccc atcactcaaa agttccaatc
    13261 gtcctacagg cgaatcatag taactcttat actccataaa aattccccct ttttctaatc
    13321 atacgcctta attgttaaat tagaaaatat tcatgttcac attttttaaa aataaataag
    13381 tcgtcaatta cgtatataaa cacgtaatac cagctatccc tttgctgcaa tatacagtta
    13441 cataacttac tacacgtgct aacctattac tttgtaaacc aaatcttaaa ttaaaatatt
    13501 gaaaatgcaa tggatcctta atattttatt aaacctataa ttacttatta aaaataacac
    13561 acaatattca taaagtttta aaaatattct gttttatcac ctattattag tggaaaagta
    13621 caattgcaat tgtatatagt ttgcataacg cttcaaaagt aatttctttt ttgtttagtt
    13681 caaaaaaatt tagaggtgat gttatatgaa taacggtttt ttcaatagcg actttgattc
    13741 aatttttcga agaatgatgc aagatatgca aggttcaaat caagtcggta acaaaaagta
    13801 ctatattaat ggtaaagaag tttcacctga agaactagcg caactcacac aacaaggtag
    13861 caatcaatct gctgaacaaa gtgcgcaagc ttttcaacaa gcagcacaaa gacaacaagg
    13921 gcaacaaggt ggcaacggca attatttaga acaaattggt cgtaacctta cgcaagaagc
    13981 acgtgacggt ttattagatc cagtcattgg tcgtgataaa gaaattcaag aaactgctga
    14041 agtcttaagt agacgaacta aaaacaatcc tatattagtt ggagaagctg gtgttggtaa
    14101 aactgcgatt gttgaaggtt tagcacaggc aatcgttgaa ggaaatgtac cagcagcaat
    14161 caaagacaaa gaaattattt ctgtagatat ttcatcatta gaagctggaa cgcaatatcg
    14221 tggtgctttt gaagaaaata ttcaaaaatt aatcgaaggt gttaaatctt cacaaaatgc
    14281 cgtactattc tttgatgaaa tccatcaaat tatcggttca ggtgccacag gaagtgattc
    14341 aggtagcaaa gggttatctg atattttgaa acctgcatta agtcgtggtg agatttctat
    14401 tattggtgca acaacacaag atgaatatcg aaacaatatt cttaaagatg ctgcattaac
    14461 gcgcagattt aatgaagtgc ttgttaatga accaagcgct aaagatactg ttgaaatttt
    14521 aaaaggtatt cgcgaaaaat tcgaagaaca ccatcaagta aaattaccag atgacgtatt
    14581 aaaagcatgt gttgacttat caattcaata tattccacaa cgattattac cagataaagc
    14641 aatcgatgtg ttagatatta cagcagcaca tttatctgcg caaagtccag ctgtcgataa
    14701 agttgaaact gaaaaacgaa tttctgaatt agaaaatgat aaacgtaaag cagtaagtgc
    14761 tgaagaatat aaaaaagctg acgacattca aaatgaaatc aaatcattac aagataaatt
    14821 agaaaacagt aatggtgaac atactgctgt tgctacagtt catgatattt cagatactat
    14881 tcaacgatta actggcattc cagtttctca aatggatgat aacgatattg aacgtttaaa
    14941 aaatatttct aatcgtttaa gaagtaaaat cataggtcaa gatcaagctg tagaaatggt
    15001 ttcacgtgca attcgccgta atcgcgctgg gtttgatgac ggcaaccgtc caattggtag
    15061 tttcttattt gtcggcccta ctggtgttgg taaaacagag cttgctaaac aattagcaat
    15121 tgatctattt ggtaataaag atgcacttat tcgacttgat atgagtgaat atagtgacac
    15181 aacagctgtt tcaaaaatga ttggtacaac tgctggttat gtcggttatg atgacaattc
    15241 aaatacgtta actgaaaaag tacgccgtaa tccatactca gtcattctat ttgatgaaat
    15301 cgaaaaagca aatccacaaa ttttaacatt gttattacaa gtaatggatg atggtaattt
    15361 gactgatggt caaggtaatg tcatcaactt taaaaataca attattattt gtacatcaaa
    15421 tgctggcttt ggcaatggca atgacgctga agaaaaagat attatgcacg aaatgaaaaa
    15481 attcttccgc cctgaattcc ttaaccgctt caacggcatc gttgaattct tacatttaga
    15541 taaagatgca ttgcaagata tcgtcaactt attattagac gatgtacaag ttacattaga
    15601 taaaaaaggt attacgatgg acgtttctca agatgcgaaa gattggttaa ttgaagaagg
    15661 ctatgatgaa gaattaggtg cacgtccatt aagacgtatt gttgaacagc aagtacgtga
    15721 caaaattaca gattattatc tagatcatac agacgttaaa catgtggata tagatgttga
    15781 ggataacgaa ttagtcgtaa aaggtaaata acgacacttt aacatatcgc gcatcaaaaa
    15841 tgagcatcag gtcgcccttg cctgtgctca tttttttaat tatttccctg gaaaatgatt
    15901 cgctgtgtgc tgttctattc cacaacaatc acgattaatg tcacatgtac cacattttcc
    15961 ttgtttcgaa cgcttgaaaa attttactag tgtatataag gcatatccga aagttgctaa
    16021 aaaaattaaa atgttaataa tgactgacac tttaaccact ccttaaacaa ataaatgtcc
    16081 gacttgataa aaaatgaatg ttaagacata tgcagtgact agaggatagg caactgcaag
    16141 tgccgtccat ttccatgaat aagtctcttt acggattgct gccactgtag aaacacaagg
    16201 aatatacaat agtataaata tcataaatgc atacgcagat agcggtgtga attgattttg
    16261 aatcacatta acaaggcctg catcacctga tgaatagata atcgccatcg aacttacgat
    16321 aacttctttt gctaaaaatc ctggcactaa cgtagcacct gcttgccatg ttccaaatcc
    16381 gagcggttgc actaacatac caaagaaact accaaccata tgtaaaaaac tttgattgat
    16441 attcacattg ataccatgtg gtcctacata acttaatagc caaatgacta ctgagccgcc
    16501 aaaaataaat gtacctgctt tacgaacaaa gcccttagcc ttttcccaag tactacgcca
    16561 caacgtttta atggaaggca cacggtatgt tggcaattcc acaataaaga ttgcattatc
    16621 attttttaaa atcgtcttag taagtactgt actgactaaa aatgccataa taatacctaa
    16681 aacatacagg cttaatacta ctaaagattg attctctttg aaaaagatac ctacgaacaa
    16741 cgcatacact ggcagtctag cagagcatga catgaatggt gcaattaata tcgttgttaa
    16801 acgctctttt tcattttcaa tactgcgcgc agccataata ctcggtacat tacaaccaaa
    16861 tccgataatc attggtataa aagacttccc gcttaaaccg aacgattcca taatacgatc
    16921 cattaaaacc gcaatacgtg ccatataacc tgaatcttct aataaagata taaagaaaaa
    16981 gagcacaaca atttgtggta caaagactaa tactgatcct acaccagcaa taatgccatc
    17041 tgtaattaaa tcttgtaaaa atggtataac ccctagataa ttcataatcg tcttcacact
    17101 atctgtaaat gtaccaccta taaatgcatc gagttgatcc gacaaaggtg tgccaatcca
    17161 tgtaaatgta gtttgaaaga tcaaccacat aattgctaga aagataggca tccctatata
    17221 tttatgtgtt aatatcttgt ctattctaga gctgaaatat tgcttatctt catctggata
    17281 cgttaccacg tcttgcaata acgtctcaat ataatgattg cgtatacgct ccatctctcg
    17341 acgaacagat acagccccta cttgttcagc aacttgatca cgtaaactcg acaatttatt
    17401 tacaacctct gaattaagtt cgtttgcaat ttgcatgtta tttaataaga attgaatcgc
    17461 aataaaccta gcttgatact tatcatgaga tgtctctgtc attattattt gacacatatt
    17521 tttaattgtc tcttcaatct tctcaccata attgatttta aaatgcggtt gatacccttc
    17581 ccctagatgc tttatttcgc caagtaaata ttttgttcct ttgcctgtac gtgccacaac
    17641 tggaaaaata ggtgttttta actttttcat caatttatga taatcgattt ttatcccgcg
    17701 ctttgtagct acatcaatca tatttaatcc gatgtatatt ggttgattaa gttctaacaa
    17761 ttgtactgtt aattgcacat ttctttttag ttgactcgca tcaacaatgt taatgattcc
    17821 tgaaaatgaa tcgtttaata aatagtctgt cactacagtt tcatctttag aaatcggcga
    17881 taaatcatat gtacctggta aatcaattaa ttgtcctaca ttttctttaa gtttccctac
    17941 ttttttctct accgttacgc cactccagtt gcctatatat tcatacgaac cagttaaagc
    18001 gttaaacaaa gatgttttac caacattagg atttcctaaa atacaataat tttccattcg
    18061 tccggctcct attcttctaa tgcaatagaa caagcatcgc aatgtctaat acttaactgt
    18121 tgtccgttta cttcaataat acatggccct ttaaataaac atttttgttt aatcgttatg
    18181 atagcgtcat ctgttaaccc aaaggcactt agacgataca acatattctc attagcaata
    18241 tccattcgct ttattttata agccttattc atttcaccat ttttaatgtt taacatacta
    18301 tttcgctctc ctattagaaa taataatcat tatcacttaa aaatcataac ccttaaaatt
    18361 gtagctcgca atactttatt taaataattt tcatttttca tgtaaaattt gcgacattgc
    18421 aaaaatgttt tgtataatca cttccatgca aaaaacctcc gcttcaaatg tcattgatag
    18481 acatttaaag cggaggcatg tacatgttat tttatgattt atttcgtaga ttttttctaa
    18541 caattgattg agcttttcaa tatttttatt caactgttct atattcgtta cttgttgttt
    18601 tgaatttagt tgatgcttgt tttgataatc tattaaagca tcatataaga acaatgaact
    18661 tgcagattgc tgtttcactt cttggtacaa tgccatcgtt ttatcatcta gtactattga
    18721 atgtgcatca tcttgcccat gtacatcttg atgtttcgat acatttgttg caccttgtgg
    18781 atatacttta aaggttgtgt cgtatgtttc cttactatct ttggcttcag attcctgtga
    18841 ttcaaccgtt ttatattttt caagtgcatg tccttcaata tcaactcgtg gaataatgcg
    18901 attcaaccat gctggtaaat accacgaacc ttttccaaac aatttcgtta atgcaggaat
    18961 taacatcatt ctgactacga aggcatcaaa gagtacacca aacgctaatg ccatacccat
    19021 tgatttaatc atgacatctt cttggaatac aaacgcaaag aagacactaa acataattaa
    19081 tgcagcggct acaataacag gaccgctttc tttcaatcct actttgatag aataatcatt
    19141 atcccctgtt ttactatatt cttcatgaat tcgcgacata aggaagactt cataatccat
    19201 cgctaatcca aataagatac ctatagtaat aaccggtaaa aatgctagca ttggtcctgt
    19261 cgtttcaata ccaaacagac ctttcataaa accatcttgc attactaatg ttgtaaatcc
    19321 tagcgttgcc attaatgaca agacaaatcc taaaactgct tttaacggta ttagaattga
    19381 acggaagaca atcattaata agaaaaatgc caatacaaca attactgttg caaataaagg
    19441 tatcgcctca tttaactttt tagacatatc aatattaatg acactttgtc ccgaaatctc
    19501 cgttttgaac ccatatttat cttgtgcatc tttatgataa tctcgtaaat catgcactaa
    19561 atcatttgtg ctctctgcat taggcccttg cttaggtatc acgaccatca aagcataatc
    19621 attatcttta ctcatttgtg gcggcgtaac gatatctaca tttttcttat ctttaatatc
    19681 tttatataca gactgtaaat cttgttgtaa tccttgtgga tcatcttttt tatctttaac
    19741 atttatcaac atcggaattt ggccattaaa tccttcacca aatttatccg agattatatc
    19801 gtaagctttt ttctgtgtag aatctgctgg tttaacaccg tcatctggaa taccaagtcg
    19861 catatgacta actggtattg cagctgctac taatatgatt aaacctagta atactgccgc
    19921 aagtgcattt cctgtaataa atttagacca cggcgtatca atatcttttt taaaattaga
    19981 ctgcaatttg ttcactttaa tgcgtttatg gaaaatacta attaacgcag gtaataaagt
    20041 cagtgcactg aatacagcaa atataacact aattgccgaa gcaaacccca tcactgctaa
    20101 gaaatctatg ccaactagcg ataaaccaca tacggcaatc acaactgtca caccagcaaa
    20161 aacaactgca ctacctgctg ttcctattgc aagaccaatg cctttaatgt aatctgtttc
    20221 agttttcata acttgtcgat acctgaataa aataaacaaa gcataatcga taccaactgc
    20281 tagtccaatc attacggcta atgtcaatgt gacatttggt atatcaaatg cataagttaa
    20341 caaactgata atacctacac cagaggctag accaatcaat gcacttataa ttggtaatcc
    20401 tgcagcaatg actgaaccga atgtgattaa cagtacaaca aatgcaacaa taataccaac
    20461 tagttcagaa ttaccgccta cttctgtaga tgtcattcct gtaccagtta actctgtttg
    20521 tacattatga ttgtcacgta agtctttcaa atgactttta acattatctc tagagccatc
    20581 ttttaaagat gtttgactaa cgtcatatgt gatatctgca aatgcagttg ttttatcttt
    20641 actaatttgc ttattttcat atggatctga tattttatca atgtgcttgt catctttttt
    20701 tatatcctct aatgttttct taatgtcttc tgtaatgttc ggttgcacaa taccatcatc
    20761 tttagtcgtc ttaaagacaa cacgtatttg tgccttttca ctatcttgat taaaatgttt
    20821 ttcaatcttt ttattcgtat ctaacgactc taatcctgtc attttaatat cattgtcaaa
    20881 tttcggtgca tttgtagcaa gtggtatcaa tattgcagct acaatcacta tccatgcaat
    20941 gaccgcggac catttatgtt ttgcgatgaa tgtccccatc ttatataaaa attttgccaa
    21001 agtatattgc ctccttttaa aatcaacctt atagtttaaa tatacagtgt agattattgt
    21061 tcgattatag tatctatccc cgacctctta aagaatcaat tggaaaattt tgtatattaa
    21121 actacacaca aaggagaaat gtagatgaaa gagactgatt tacgagttat aaagacaaaa
    21181 aaagcattgt cgagtagctt gctacaattg ttagaacagc aattattcca aacgattact
    21241 gtcaatcaaa tttgcgacaa tgcactcgta caccgcacaa cattttataa acatttttat
    21301 gataaatatg atcttctaga gtatttgttc aatcaattga ctaaagacta ctttgctaga
    21361 gatatcagtg accgtcttaa tcatccattc caaacgatga gtgatacgat taataataaa
    21421 gaagaggatt tgagagaaat cgcagaattc caagaagaag acgctgaatt taataaagta
    21481 ttaaaaaatg tctgcattaa aattatgcat aacgatatca aaaataatag agaccgtatc
    21541 gatattgaca gcgacatccc agataatctc atattttata tttatgactc gttgattgaa
    21601 ggttttatac attggataaa agatgaaaaa attgactggc ctggcgaaga tattgataac
    21661 attttccata gattaatcaa tattaagatt aaatagtaga tgagaaactc atgagcgtta
    21721 ccaacattca taataaaaac gatagtgtac acgttaatga attcgtgtac tactatcgtt
    21781 ttttattttt atcgtgctta tcgttattaa aataactgat acacaacaca taaactatga
    21841 agaaaaaaat aaatccgcta tctaaatgac tttgactcag ttgtttatat gaccaaattg
    21901 ctaatacaat tcccattatt attgaaataa cgtatctcac attcttatac ctataatcct
    21961 tttctaaaaa tatggttgct attacttaat ttttaaagtt ataaataaaa agagccaacc
    22021 gcaatggatg gcccttgttc attatgaagc attagaacat ttctgaaaca accttttgtt
    22081 ctaagaagtg taataagtag tctgggctac ctgttttagc gtccgtacct gacattttga
    22141 aaccaccaaa tggatggtat ccaacaactg ctgaagtaca acctctgtta aggtataaat
    22201 tacctacatc aaattcgttt accgctttaa tccaatgctc gcgattattt gtaatcactg
    22261 caccagttaa accgtaatct gtatcatttg caacctcaat ggcttcatca aaatcgttaa
    22321 ctttcacaaa gccaacaact ggaccaaaaa tttcttcttg catgattcta tctttagatt
    22381 taagtcctga aatgattgtt ggttctacaa agtaaccttt tgaatcatca gtgccgccac
    22441 cttgttctaa tttaccttct tctttaccaa tctcaatata atttttaatc ttatcaaatt
    22501 gttttttatt aataactggg cccatatacg tattgtctac agtattgccc aacgttaatt
    22561 cttttgttaa tttgattgat ttctctaata cttcgtcata aacgtcttta tgcacaattg
    22621 cacgtgaaca tgcagaacat ttttgaccag agaaaccaaa cgctgacgtt acaatagctt
    22681 ctgccgccat atctgtatca atattttcat caactacaat ggcatcttta ccacccattt
    22741 cagcgataac acgtttcaag aagttttgac cttcttgaac tacggcacta cgttcataaa
    22801 ttctagtacc tgtcgcacgt gatcctgtaa atgtaacgaa atgcgtatct ttatgatcaa
    22861 ctaagtaatc accaatttct ttcggatcac caggaacaaa gttaactacg ccttttggta
    22921 atcctgcttc ttctaaaatt tccattaatt tataagcgat ataaggtgta tcctcagcag
    22981 gtttcaataa cactgtatta cctgcaacaa ctggtgctaa agtcgtacca gccataatcg
    23041 caaacgggaa gttccacggt ggaattgtaa cacctgtacc aattgattta tagaaatatt
    23101 tattgtgttc accttcacga tcaagtactg gcttaccttg agccaaatcc atcattgaac
    23161 gtgcatagta ttcaataaaa tcaatacctt cagctgcatc accaactgct tcatcccatg
    23221 gcttacctgc ttcataaacc ataattgctg caatttccgc ttttcgacga cgaataattg
    23281 ccgaaacacg taacataagc tctgcacgat catttgctga ccatgttttc caagatttat
    23341 aagcttcgtt tgctgcttta aatgcatctt caacatcttg ttttgttgcc tttgatgcat
    23401 ttgcaatcac ttgtgatgtg tctgcaggat tgattgattt aattttgtca tctttgaaaa
    23461 tcttctctcc attaatcact aatggtatgt cttgacctaa ttctttttcc acgtctttca
    23521 atgctttctt aaacatatcc acattttctt ggactgaaaa atcgtaacca ggttcatttt
    23581 taaattctac taccatgtac acttaccccc tataaatttt gaaagtggtt taaccctttg
    23641 atttaatgat ataacatcat ttaaactcat tttactatga ttaaggttag ttttgcaatc
    23701 gctttcattt ttatgcttta tcacttattc tcaagtattt tgaaattgat tggttacttt
    23761 ttaaaattta tatgggtcgc aactgctact ttatcgtttc gtcatttaat gtttcggatg
    23821 gtaggtcatt atcaatttta cgaacgactt tacaagggtt tccaaccgct aagctgtgtg
    23881 gcggaatatc tttagtgaca acactaccag caccaatcac actgccttct ccaatcgtca
    23941 cccctggtaa cacggctaca tgaccgccaa accaagtatt actgccaata tgaatgggtc
    24001 cggctttttc ataaccttca tttctatgat aaaaattaag tggatgtgtt gctgtgtaga
    24061 atccacaatt aggtcctata aaaacattat cgccaattgt aatctgtcca ccatccataa
    24121 aataacaatt ggtgtttaca tagacatttt ttcctagttt aacgttccaa ccataatctg
    24181 tatcaaaagg aatcgaaata cttacattgt ctgttgttgt ttgaaataat tgatcgatta
    24241 attcctttct tttatttgta tcactcggct ttgtatgatt taattcaaag caaatatctt
    24301 tcgctcgtgc acgttcattg attaagtctt gatcaaagtt tgcatcgtac catttttctg
    24361 ctaacatttt ttctttttca gtcattacac ctttcaactc ctaataactt atttacttgt
    24421 ttaaaagtta atcaaataaa cattcgccta tgcaactaat acgctataac attatgaaat
    24481 catgacctta tcacccttat ctatacaatt ctcgcatcaa atactgctaa agtagtagat
    24541 aaattcaata ctacagacgc attcattttt taatctatta acgtacaatg tgagtaagag
    24601 aaatataaag gagtatgata gcgatgagaa tattaattac aggcacagtt gctatcttaa
    24661 tcattctagg tttagttaaa acgatacaag attacgaaat gacaaacgat acaagtcgtc
    24721 agttgtcaga caacaaagat gatgataaag tcatccatct taataatttc aaaaatttac
    24781 atgcgaaaga atttaaccca tctgatttct tttaagtcac ctaagaattg caaatccaga
    24841 agtcatttaa gttttacctt tcattcatac atcctttaat attaattacg acttctttta
    24901 tatagatgct aaatagagag attgttgtgc aatgtttgca cggcaatctc tctttttctt
    24961 tttaaaattg gtaaaagtaa aacgcaacga ttgacttata tacctagagg gggtacatta
    25021 gacgtgtaac aatgaatcac agggaggcaa taatgtggct aatacgaaaa aaacaacatt
    25081 agatatcact ggtatgactt gtgccgcatg ttcaaatcgt atcgaaaaga aactgaataa
    25141 acttgatgac gttaatgccc aagtgaattt aactacagag aaagcaactg ttgagtataa
    25201 ccctgatcaa catgatgtcc aagaatttat taatacgatt caacatttag gttacggtgt
    25261 cactgtagaa actgtcgaat tagacattac aggtatgact tgtgctgcat gctcaagccg
    25321 tattgaaaaa gtgttaaata aaatgaacgg cgttcaaaat gcaacggtca atttaacaac
    25381 agagcaagct aaagttgact attatcctga agaaacagat gctgataaac ttgtcactcg
    25441 cattcaaaaa ttaggttatg acgcgtctat taaagataac aataaagatc aaacgtcacg
    25501 caaagctgaa gcgctacaac ataaattgat taagcttatc atatcagcag tattatcttt
    25561 accactatta atgttaatgt ttgtgcatct tttcaatatg catataccag cactatttac
    25621 gaatccatgg ttccaattta ttttagctac acctgtacaa tttattattg gatggcaatt
    25681 ttatgtaggt gcttataaaa acttaagaaa tggtggcgcc aatatggatg tacttgttgc
    25741 tgttggtaca agtgcagcat atttttacag tatttatgaa atggttcgtt ggctaaatgg
    25801 ctcaacaacg caaccgcatt tatactttga aacaagcgcc gtactactta ccttaatctt
    25861 attcggtaag tatttagaag ctagagcgaa gtctcaaaca accaatgcgc ttggcgaatt
    25921 attaagttta caagctaaag aagcacgcat tttaaaagat ggtaatgaag tgatgattcc
    25981 tctaaatgaa gtacatgttg gagatacact tatcgttaaa ccaggtgaaa agatacctgt
    26041 tgatggcaaa attattaaag gtatgactgc catcgacgaa tctatgttaa caggtgaatc
    26101 tatccctgtt gagaagaatg ttgatgatac tgtaattggt tcaacgatga acaaaaacgg
    26161 tactattact atgacagcaa caaaagttgg cggggacact gcgttggcaa atattattaa
    26221 agttgtcgaa gaagctcaaa gttctaaagc gccgattcaa cgattggcag atattatttc
    26281 tggttatttc gttcctatcg ttgttggtat cgcactatta atatttatcg tgtggattac
    26341 tttagttaca ccaggtacat ttgaacctgc acttgttgcg agtatttccg ttcttgtcat
    26401 tgcttgtcca tgcgcattag gacttgctac accaacttct attatggtag gtactggtcg
    26461 cgctgctgaa aatggcattt tatttaaagg tggcgagttt gttgaacgca cacatcaaat
    26521 tgataccatc gttttagata agacgggtac cattacaaat ggtcgtccag tcgtgacaga
    26581 ttatcatggt gacaatcaaa cgctacaact gcttgctact gctgaaaaag attctgaaca
    26641 cccattggca gaagccattg tcaattatgc aaaagaaaag caattaacat taactgagac
    26701 aacaacattt aaagcagtac ctggccatgg tattgaagca acgattgatc atcaccatat
    26761 attggttggt aaccgtaaat taatggctga caatgatatt agcttgccta agcatatttc
    26821 tgatgattta acacattatg aacgagatgg taaaactgct atgctcatcg ctgttaatta
    26881 ttcattaact ggtatcatcg cagtggcaga tactgtcaaa gatcatgcca aagatgctat
    26941 aaaacaattg catgatatgg gcattgaagt tgccatgtta actggcgata ataaaaacac
    27001 tgctcaagcc attgcaaaac aagtaggcat agatactgtt attgcagata ttttaccaga
    27061 agaaaaagct gcacaaattg cgaaactaca gcaacaaggt aagaaggttg cgatggttgg
    27121 tgacggtgta aatgatgcac ctgcattagt taaagctgat atcggtatcg ccattggtac
    27181 aggtacagaa gttgccattg aagcagctga tattactatt cttggtggcg acttgatgct
    27241 tattcctaaa gccatttatg caagtaaagc aaccattcgt aatattcgtc aaaatctatt
    27301 ttgggcattc ggctataata ttgccggtat ccctatagct gcattgggct tacttgcgcc
    27361 atgggttgct ggtgctgcaa tggcactaag ttcagtaagt gttgtcacaa acgcacttag
    27421 attgaaaaag atgcgattag aaccacgccg taaagatgcc tagattcctt aataatgaag
    27481 gattcgttgg tgattctgag ataggctagt gattggctct ataatgtcgc ggtttacagt
    27541 tgggtcttcg ctccaactgc atatatagtt acacttttcg cttagcgaat tagtgtatct
    27601 tacctaatag cttcgcctat taggttccat cattattata aataataagt acactacggt
    27661 ttacagttgg atcttcgctc ctactgcata tatagttaca cttttcgctt ggcgaattag
    27721 tgtatcttac ctaatagctc cgcctattag gttccatcat tattataaat aataagtaca
    27781 ctatggttta cagttggatc ttcgctccaa ctgcataaga gcctgagaca taaatcaatg
    27841 ttctatgctc tacaaagtta taatggcagt agttgactga acgaaaatgc gcttgtaaca
    27901 agcttttttc aattctagtc aactttgccg gggtgggacg acgaaataaa ttttacgaaa
    27961 atatcatttc tgtcccactc cctaaaagga ggtgaatgct atgtcacaag aaattttaaa
    28021 tgttgaaggt atgagctgtg gtcactgcaa aagtgctgtt gaatctgcat taaataatat
    28081 tgacggtgtc acttcagctg acgttaacct tgaaaatggt caagtaagtg ttcaatatga
    28141 tgacagtaaa gttgctgtat ctcaaatgaa agacgcaatt gaagatcaag gttacgatgt
    28201 cgtttaatta ggcaatattc aacgtcatca acaccaaatt aaaaaaatcg aactgatgag
    28261 aatcccaaca atccaaatta tctcatcagt tcgattttta atttactcgt aacctagtat
    28321 ctccagtctg caatacatct aatgttgcat ctaatgcatc gacaattaga tttttaactg
    28381 cagcttcagt ataaaacgca atatgtggtg ttaatatgac atcttccctg tcaatcaacg
    28441 attctaacaa tggatcgttc ggtgttttgc ccctttgatc acttgggaaa agtttgcgtt
    28501 caaattcata cgtatcaagt gctgcacctt taatcacacc attgtctaat gcgtctaata
    28561 acgccttagt atctactaaa gaacctctcg cacaattgac aaatactgcg ccctttttaa
    28621 aatgtttaaa taattcagca ttaaatagat aatgattata tttcgttgca ggtacatgta
    28681 atgtcacgat atcagcacct tcaaccgctt cctcaatcgt atctttgtaa tcgacatacg
    28741 ttgcaatttt agcattagga aacgggtcgt atgcgaccac atcactttga taaccattgg
    28801 caaatatatc ggctactaca cggccaattc gacctgtacc aataacagct acttttaaat
    28861 ctttaatgga tttcgataaa atagtaggtt cccatctaaa atcatgctcc cgcactttcg
    28921 tttgaatttg attaaaatga cgaaccacat taatagcctg gttcacagca aactccgcaa
    28981 ttgaattcgg agagtatgac ggcacatttg acacaatcaa gttatacttg tttgctaact
    29041 ccaaatcata tgtatcaaat ccagcactac gttgtgcgat ttgtttaata cctagttcat
    29101 ttaatcgttt ataaacatgc tctgataatg gtatttgttg tgatagcgat aagccatcat
    29161 aaccagcgac accttcaaca ttgtcatcag ttaatgcttc tttagtaata tctacctcaa
    29221 catgatgttt ctctgcccac gccttgatat aaggcatatc ttcatcacgt acactcatga
    29281 ttttaatttt tgtcatttta acatcaccct taactttatt attcatataa atatgctagt
    29341 tctgttaatc ttattgcagc ttcgtctaat ttctggtcat ctaacgccaa tgaaattctc
    29401 acataacgat taccattctc tccaaatggt ttccctggag caacaagtat tgacttctct
    29461 tgcactaaaa attgctcaaa ttgctcgctg tcataaccag gcggtgtttc caaccataca
    29521 tatatgccac ctttagcatg aacaaatggc aaatcagctt ttgcaagcat ggcttcgaat
    29581 cggtcacgac gtgttttaaa tacattgctt tgttcttcta aaaaatcatc ataatgattc
    29641 aaagcatata ttgcggcatc ttgtaatgca ccaaacatcc cagcatttgt gtgcgtttgg
    29701 tactttttca aagcttgaat catatcttta ttaccaactg caaaaccgac tctaaaacct
    29761 gacatgttat aacctttaga caaggaataa atttcaatcg caacatcttt cccattttcc
    29821 gaagcaagta tactaggatt tttagcgtcg aaaccgaaag caccataagc aaaatcatgc
    29881 acgattttag tgtctgtacc tttaaattta gctatcgctt catcaaaaac ttctttcgta
    29941 gctgtcgatc cagttggatt atttggatac gttaaataaa tgagttttgt tttatctatt
    30001 atttgtgaat caactgtgga ccaatctggc aaataatgtg gcggttctaa attaagcgga
    30061 actggcttgc catcagctaa aagtacacct gctaaatagt ccgtgtagcc tggatcaggt
    30121 agtaatacat agtctcctgg attgataaca catgttggta ctgccactaa tccatttttt
    30181 gtaccatata aaatgcatac ttcatcttct ttatctaacg tcacattata ttgtctttga
    30241 taaaaatcta caatagcttg cttgaacgct tctttaccat gaaaagcacc atatttttga
    30301 ttttcaggaa tagttagtgc tttttgaaaa tgatcaataa taccttgtgg cgtgggccca
    30361 tcagggatcc caactgccat attaattaat ggcaatggtc catgttcgat tttacgtccc
    30421 atcgttttcc cgaaataact atcagggata tttgctaatt tgttagagat catcaaattc
    30481 ctcctctatc attaaacata gcctgggcga ctatcataat cctaacaact tgtatcactc
    30541 tcatttagat ggttacaatg acatcgccat tcaccgttat gttcaacaga acttatgaca
    30601 cacgttgtat tgaatgaatt tattttcatt ttaggtaggt acattattat tgtcaatatt
    30661 aggaattttc agattaatat gcactcaatc gttatgattt aactgtcatg catatccgca
    30721 tgcgcaacca gttagatatg cttatataaa gtataacgcc cctcaaggta cgtattcaaa
    30781 cgtgaacctt aacaggcgcc tattcattgt taaataaaac ttctttagaa catacttatt
    30841 tcactatgcc ttttactttc cccttatact tttctcacat ctttctctta gactactccc
    30901 ttatacgccc cgctcaatat ctttaatcat ttcgtctaca gttattttcg cacttgttaa
    30961 gacaatagga acgcctgcac ctgggtgcgt acttgcacct gcaaaatata aatctttata
    31021 atctcgcgat acattttgtg gacgataata attactttgc gctaaagttg gcattaaacc
    31081 gaatgccgaa ccaaatttcg catgatacgt ttgctcaaaa tcatttggcg taaagattgt
    31141 ttctgaaaca atatgcgatt ttatatcttc aaatacttca attgttgcta atttacgata
    31201 aataatttcc tttatttgtt gcgtcaaagc ttcatctgac caatcgattc cgctacctgt
    31261 tttaagttcc ggcgtcggca ttagcacata aataccagtt ttgccttctg gcgcaagtga
    31321 tttatcagcg accgctggta catacacata aatagaagga tcatatgata aacgtccctc
    31381 aaatatttct tcaatattgc ctctaaagtc atctgaaaaa ataacattat gaagtctcac
    31441 ttgatctgtc acatcaatat ctataccgat atacattaaa aatgctgaac aagagtaatc
    31501 taagtctgca attttatgtg gtggatactt tttaataggt gcaaaatctg gcattaatga
    31561 ttccgcaaca ctagggaaat cagccgtaca taaaatttta tcaaattttc ttatgtcacc
    31621 attcactttt atcgcatcgg cccgtttgaa tttaggatca ataataattt gctcaatttc
    31681 agcatttagt tcaatattaa cgcctaagtc tttatttaat tgcgctagcc cttgagccat
    31741 accatacata ccgcctttaa taaaatgcac accaaacatc atttcaatca taggaataat
    31801 tgaatatagt gacgggcctc gttttggatc aattcctatg tataacgttt gaaacgctaa
    31861 aagcttttgt atcttttcgt tatcaatata atgttcaatt agctgatctg catgatttaa
    31921 cgtttttaac ttagcacctt gcacaagtga cgtcatatta taaaagtcac tcggtttgcg
    31981 atacgttctt tctaagaaat agcgacgtgc aatttcatat tttttataaa catccgttaa
    32041 aaaggacata aaaccatgcg ttgaaccagg ttctatactt tctagcattt gctgtaattc
    32101 agctaaatct gtaggcaccg ttatacgatc atcgtggtca aaatacacat cgtaaatata
    32161 acgtaattgt ctcaattcaa tataatcttc ataattttta ccacacgctg taaaaacatc
    32221 tttataaaca tctggcatca tgacaattgt gggacccata tcaaatgtaa agccgtcttt
    32281 ctttaattga ttcatacgcc cgcctacatt attatttttt tcaaatatcg tcacttcatg
    32341 accttgagaa gcaatacggg ctgccgctgc taatcctgtg acacctgcac caattactgc
    32401 aatcttcatt attcaaccac ctatattcta tgatatttac tatttatttc atgaaacaac
    32461 tttgcctttt tccgcttatc cacaaaaaca cgttcatgta atgtatagtt agcctgtctc
    32521 acttcgtcca gtatttcaat atatatacgt gctgctaatt ctatgattgg ttgtgcttca
    32581 atactaaata ctttgatttg atccataaca tcttgaaaat ctttttctgc gatagctgca
    32641 taatattccc ataagtcaat ataatgatta ttaacaccat tttggtacac ttcagcaata
    32701 tcaacttcat attgctttaa tcgttgctta ctaaaatata cccgttcatt gtcaaaatct
    32761 tcaccgacat ctcttaatat attaatcaat tgcaacgatt caccaagtct tcttgcgaca
    32821 tcgtatgtct gatgtgtttc atgatcactt aaaatcggcg tcaatacttc acctactgta
    32881 ccagcaacac cataacaata tccgaataat tcagcgtccg tttcaaacat tgtaaaatgt
    32941 tgatctttat atacagtatc aatgagatta taaaaagatt gaaaggcgat atttttatgt
    33001 tgtgcaacat gctgaagcgc catcatgatt ctacgatcac tttgaaagtg atgatgttca
    33061 tatgggtatt tttcaataga ttgtatatct tcttttattt gatttaaaaa ttgaatatcg
    33121 ccataaacat ctatactgtc atcaatttta cgacacacag cataaattgc ccaaaccgct
    33181 tttctttgat cttctggtaa caagtcaaaa gcgtaagaaa agctttttga atgtttcttc
    33241 atgattttat gacaatattt aaaattcata tccatcattg tcatactagt cctcctatat
    33301 tgaaatgcca ttaccttgat ttattgttct ttagatatag aatattgacg acctttccat
    33361 tcaactacac cataacgatg cgtttgtttc caagattgga tgaaaatttt agtaaaaaac
    33421 ataaataaca atggatgaca taccattaat aaattagaaa attggccgac ccttcgatgc
    33481 agataaataa attgtgtagt atagctcaag taaagtgcta ccatttttct tacagacatt
    33541 tggcgatatt ttaaactaag acatagccct aaaatactcg ctatagaacc gaacaaccac
    33601 aacacaattg ctgtcatgat cttaggcttt gtgccacctg ccccagttga caaatgcttt
    33661 gtccatcctt cttgtaatga ctgaaatcct tcttgataca tgcgaaatgc aacaaatgga
    33721 aatccttcat aaactgttac gggcaatgat tgtgaagtat atgcacttcc taaagcaaat
    33781 ccttcaataa tatgacggtt cgcactttta tgacctccag ttgcataata atcttcttta
    33841 tttgttaatg tcaccggtcc aaatgcatgc tggttagtcc gaccgtcgtc taaggtagaa
    33901 aatacattca taccaacgac tgtcattaaa ttaaatatcg ctgaaaaccc ttcgtagaaa
    33961 cgctttgtta tatgataagg ctgtacgctt aacaatcctt tttcaccttg taattgatac
    34021 tgattaatca acgtttcaac agcatctttc cttaagaaag ttacatcagc atctacaaag
    34081 gcaatacgat tcgtacatgc atgcgtcaca ccttgataac aagcatgtga tttcccatac
    34141 catttgccgt cagtatcatc aacaacatct actacagtcg caccatatga acgtgctaca
    34201 cgtgctgttt catctgtcga tccgtcattc ataacaatga catctactgg aacttgctgt
    34261 tgtattatcg aatgtagtaa atgacctatt cttttttctt catttctagc cggaataata
    34321 attgttaatg ctttatgatt gaagttcagc gttttcgtct ttagctgatg tctacgatta
    34381 aatatcaatg caccacacgc catagacatg gtcactatta ctgttaatat tcgtgataac
    34441 catttcatag atatcacttc ctattcttcg cttctcgcgc gtttattttg tctgcgactt
    34501 gttgcccact taatgtaacc attggcatgc caccaccagg atttactgat ccacctacaa
    34561 agtacaagtt ttcaaaatac tgactttctt taggaaattt aaatcctttg tttttctttt
    34621 tatctgctac aacaccatat attgcaccac gattagaacg ataatttttt tcaatatcct
    34681 ccggtgtcca aacatcttca taaataatgt gttttcttaa atcagtaagt cccatttttt
    34741 ctaatttatc caaaatttta tccctaaact tcgcataatc ttcagtggta aaaggctgat
    34801 cttgaatata tggaatatgt ggtaagactt tgatattttc ataacctact ggcgcttgtg
    34861 tgtgatcagt tttattcgta tttactagat aaatggtcgg atcatctggc aatacctttt
    34921 catgaaaaac ttgttgataa ttgagataag cattttccgt aaaaaagaaa ttatgatgtg
    34981 ctaattgcgg gtattggcaa gcaacaccta aatgcatcac atatcctgag cttgccggct
    35041 caaattccct ctctaatttg tttaatcgtt gagtatcaag gtgaattaaa tatttataag
    35101 taggtatgac ttccatattt gaaataatat aatctgcctt tacaaactca cctgtatcta
    35161 atctgacacc cgtcacacgt ctttgatatg ttttaatatt gtccacacgt gcacctgtat
    35221 gaattgtgac accttcttca cgcgctagct tttccaaggc attggctaaa tgatggattc
    35281 caccttctac ataccaaagg ccttgctctt gttgcatatg gaataacata gataatacag
    35341 ctggcgcatc gtatgatgaa gaacctacat atttgataaa atagcctaac atttgtcgca
    35401 agtatggatt cgagatacgc ttgtctatgg cctgttgcat agtatgtaca taatcataat
    35461 taataagagc atttaatggc ccatgaaatt taataatttg agatagtgta tctaaaccat
    35521 agttaaaata ccctttttca gtaatacgat cgattcgtct tgtataattc aaataatttt
    35581 gcagttcctc tatatcctgt ttcgacaata tcgcattatg ctgacctgtt tctttaatac
    35641 cttcatacaa atcgatagtc gtgccatctg gaaaaaagct acgccattga tgtggcaaac
    35701 gcttgattgt aacgtagtct gacatttgct tcttgctata ttcgaataat ttttcaaaaa
    35761 tataaggcat cgttaaaata gatggaccta aatcaaagcc aaagccatct gattcatgac
    35821 gattcacttt gcctcctata tgagtatttt gttcatataa tgagaccgaa tagccacttt
    35881 gtgccattcg aattgctgca gaaatcccac ctaagccacc accaataacg atgatatgtt
    35941 tagtcatgac gttcacctct tcgttttaat atgcgtagta actgcgttaa tctcggtcga
    36001 ttatagcgtt gcacaataat gattggaaca ttagctatca ttgcatagaa aatatttata
    36061 tactttacta aacgagagcc tttattgaat atgatgactg gaagtatcga tatccaatga
    36121 atcaactctg ccctctttgt ctctataatc attttttcaa tttcatcagt attgattgtc
    36181 attaaatgac gctgatcata tatattttga ttaagctgat gaccatctaa aatcttatgt
    36241 ttccattttc ttacgtaaaa ccatttgttc caaaattttc catgcttctc aaaattaaat
    36301 gatttaaata ttatgtactt ctgacgaaaa tacttatcag gaattcttgt acctaaattt
    36361 gctatattta gttgaacaat taaccaatac atactacaaa aaaatgctgt tttgatatat
    36421 tttttcatgg ttttcatcta aattgaatca ctctcaatca tactgacata gtataaatta
    36481 tcactttatt acttagttcc ctaaagatca gtgaataaac cgctctcatt tatatttata
    36541 taaaacaaaa acgccaatac tctcatgtta gagaatattg acgtcatttg atttacattt
    36601 gattaatgaa tgaaattata tgaacctgct tggtttgctg aaattgtacg agacgtaacc
    36661 acaccagcac catgtccata gttcatttct gaaacacgaa cagaaccgtt gctgttaacg
    36721 ccttcaacgt aagcaacatg accgtaatag ccttgtgttg tttgcatgat agcaccaact
    36781 tttggtgtat tgttcactgt atagccagat gatgcagctg cgttagccca attacttgcg
    36841 ttaccccatg ttgaaccaat tttcccacca acacgatcaa atacataata agtacattgt
    36901 cctgaagtat ataagttact tcctgatgca taaccattag aaattgaacg accatttgaa
    36961 gatggcgctg cagttgtagt cacatgaaca ttattacttg ttgtgctata acttgcacct
    37021 gaaccaccag tataatagtt atttgttgct gtttggctat tgtttgtata gttattgtat
    37081 tgatatgaat gattatagta gttattgtaa gatgcattgt tataactata agtattgtag
    37141 ttgttgtagt agtatgtgtt gttttgcgtt aattgacttg gattccaatt tcctgtccaa
    37201 gtgtaatgat aattaccttg tgcatcaatt gtatacgtat agctgtatga ttgagcgtca
    37261 ttagaattat atccattgtt attttgttct gcggcttgtg catcatgtcc tgcaaatgcg
    37321 atagtggcaa gtcctgctgt agcgattgta gctgtaacga tttttttcat aattaaatat
    37381 cctcccaata atcaaaacac tctatacata tagtcattag cga
//
LOCUS       DANBWH010000003        37296 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.003, whole genome shotgun sequence.
ACCESSION   DANBWH010000003 DANBWH010000000
VERSION     DANBWH010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 37296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37296
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.003"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            41..994
                     /locus_tag="PPY92_000089"
     CDS             41..994
                     /locus_tag="PPY92_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624121.1"
                     /translation="MKKTLGCLLLIMLLVVAGCSFGGNHKLSSKKSEESKQETVKKES
                     EEEKDPDLEKYEEIEKKMKGIKDAPSLDKLDPLMTEKSFTNSKGIQGWKDYKELMGKV
                     ELADYRFTKDSKGSSIKDVDAFFKGKKGIKRKVIETHDDVKQVDYWYVDPDGKKIGNS
                     NTPVFYAEIMTKYKDGKLVYASVEPGSYVIHKDDAIKYDDYSKLKKLSQLTKLDHPKP
                     VPYSVAQIKSFGVPLTSVSFMTHGSKDTKDEVLPALAYFTFSPKNYEDKSNPDPKVLN
                     LVHMDFLNASSDFGNAHFVVLSKYIKEYESNYETASDDSLK"
     gene            complement(1157..1360)
                     /locus_tag="PPY92_000090"
     CDS             complement(1157..1360)
                     /locus_tag="PPY92_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HDJ2624122.1"
                     /translation="MQVNKVIYILLALFLGSFGIHKFYAGKNMQGILHLIFCWTGIPH
                     ILAIISAVITVFKPADEQGNVTL"
     gene            1659..2147
                     /locus_tag="PPY92_000091"
     CDS             1659..2147
                     /locus_tag="PPY92_000091"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDJ2624123.1"
                     /translation="MAIKKLVPASHPILTKKAQAVKTFDDSLKRLLQDLEDTMYAQEA
                     AGLCAPQINQSLQVAIIDMEMEGLLQLVNPKIISQSNETITDLEGSITLPDVYGEVTR
                     SKMIVVESYDVNGNKVELTAHEDVARMILHIIDQMNGIPFTERADRILTDKEVEAYFI
                     ND"
     gene            2140..3075
                     /locus_tag="PPY92_000092"
     CDS             2140..3075
                     /locus_tag="PPY92_000092"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDJ2624124.1"
                     /translation="MTKIIFMGTPDFSTTVLEMLIAEHDVIAVVTQPDRPVGRKRVMT
                     PPPVKKVAMKYDLPVYQPEKLSGSEELEQLLQLDVDLIVTAAFGQLLPESLLALPKLG
                     AINVHASLLPKYRGGAPIHQAIIDGEQETGITIMYMVKKLDAGNIISQQAIKIEENDN
                     VGTMHDKLSVLGADLLKETLPSIIEGTNESVPQDDTQATFASNIRREDERISWNKPGR
                     QVFNQIRGLSPWPVAYTTMDDTNLKIYDAELVETNKINEPGTIIETTKKAIIVATNDN
                     EAVAIKDMQLAGKKRMLAANYLSGAQNTLVGKKLI"
     gene            3072..4379
                     /gene="rsmB"
                     /locus_tag="PPY92_000093"
     CDS             3072..4379
                     /gene="rsmB"
                     /locus_tag="PPY92_000093"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDJ2624125.1"
                     /translation="MIENVRSLAFDTIQDILNEGAYSNLRINEVLSENELNAMDKALF
                     TEIVYGTVKRKYTLDFYLKPFVKTKIKAWVRQLLWMSIYQYVYLDKVPNHAIINEAVE
                     IAKERGGYHNGNVVNGILRTMMRSDLPDFNEIADPKKRMAIEYSMPKWIIDHWATHYG
                     LEETETILQSFLETTSTTVRANLTRASLDDIIEKLQDEGYDVEKDHDLPYCLHIGGQP
                     IIHSRSFKDGFVSIQDKSSMFVAHIMNVDRHDHVLDACSAPGGKACHIAEVLMPEGQV
                     DASDIHDHKIDLINFNIKKLRLTNIKAFQHDATKPYDKTYDKILVDAPCSGLGVMRHK
                     PEIKYTQSKQHIESLVELQLEILENVKNNVKIGGEIIYSTCTIEQLENENVIYTFLKN
                     NKNFEFEPFQHPITGELVKTLQIMPQDFNSDGFFITKIKRKDN"
     gene            4382..5476
                     /gene="rlmN"
                     /locus_tag="PPY92_000094"
     CDS             4382..5476
                     /gene="rlmN"
                     /locus_tag="PPY92_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002435140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDJ2624126.1"
                     /translation="MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAK
                     QIFEWLYQKRVDSIDEMTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLFELQD
                     GYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKA
                     LDATEERVSQIVIMGIGEPFENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIY
                     DFADEDIQINFAVSLHAAKDEVRSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYG
                     LFGGVNDQLEHARELAHLIKGLNCHVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLG
                     INATIRREQGSDIDAACGQLRAKERQVETR"
     gene            5483..6226
                     /locus_tag="PPY92_000095"
     CDS             5483..6226
                     /locus_tag="PPY92_000095"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase Stp1"
                     /protein_id="HDJ2624127.1"
                     /translation="MLEAQFFTDTGQHRDKNEDAGGIFYNQTNQQLLVLCDGMGGHKA
                     GEVASKFVTDELKSRFEAENLIEEHQAENWLRNNIKDINFQLYHYAQENAEYKGMGTT
                     CVCALVFEKSVVIANVGDSRAYVINSRQIEQITSDHSFVNHLVLTGQITPEEAFTHPQ
                     RNIITKVMGTDKRVSPDLFIKRLNFYDYLLLNSDGLTDYVKDNEIKRLLVKEGTIEDH
                     GDQLMQLALDNHSKDNVTFILAAIEGDKV"
     gene            6223..8217
                     /gene="pknB"
                     /locus_tag="PPY92_000096"
     CDS             6223..8217
                     /gene="pknB"
                     /locus_tag="PPY92_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase Stk1"
                     /protein_id="HDJ2624128.1"
                     /translation="MIGKIINERYKIVDKLGGGGMSTVYLAEDTILNIKVAIKAIFIP
                     PREKEETLKRFEREVHNSSQLSHQNIVSMIDVDEEDDCYYLVMEYIEGPTLSEYIESH
                     GPLSVDTAINFTNQILDGIKHAHDMRIVHRDIKPQNILIDSNKTLKIFDFGIAKALSE
                     TSLTQTNHVLGTVQYFSPEQAKGEATDECTDIYSIGIVLYEMLVGEPPFNGETAVSIA
                     IKHIQDSVPNVTTDVRKDIPQSLSNVILRATEKDKANRYKTIQEMKDDLSSVLHENRA
                     NEDVYELDKMKTIAVPLKKEDLAKHISEHKSNQPKRETTQVPIVNGPAHHQQFQKPEG
                     TVYEPKPKKKSTRKIVLLSLIFSLLMIALVSFVAMAMFGNKYEETPDVIGKSVKEAEQ
                     IFNKNNLKLGKISRSYSDKYPENEIIKTTPNTGERVERGDSVDVVISKGPEKVKMPNV
                     IGLPKEEALQKLKSLGLKDVTIEKVYNNQAPKGYIANQSVTANTEIAIHDSNIKLYES
                     LGIKQVYVEDFEHKSFSKAKKALEEKGFKVESKEEYSDDIDEGDVISQSPKGKSVDEG
                     STISFVVSKGKKSDSSDVKTTTESVDVPYTGKNDKSQKVKVYIKDKDNDGSTEKGSFD
                     ITSDQRIDIPLRIEKGKTASYIVKVDGKTVAEKEVSYDDI"
     gene            8445..9320
                     /gene="rsgA"
                     /locus_tag="PPY92_000097"
     CDS             8445..9320
                     /gene="rsgA"
                     /locus_tag="PPY92_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDJ2624129.1"
                     /translation="MKTGRIVKSISGVYQVDVNGERFNTKPRGLFRKKKFSPVVGDIV
                     EFEVQNINEGYIHQVFERKNELKRPPVSNIDTLVIVMSAVEPNFSTQLLDRFLVIAHS
                     YQLNARVLVTKKDKTPIEKQFEINELLKIYENIGYETEFIGNDDDRKKIVEAWPAGLI
                     VLSGQSGVGKSTFLNHYRPELNLETNDISKSLNRGKHTTRHVELFERQNGYIADTPGF
                     SALDFDHIDKDEIKDYFLELNRYGETCKFRNCNHIKEPNCNVKHQLEIGNIAQFRYDH
                     YLQLFNEISNRKVRY"
     gene            9321..9965
                     /locus_tag="PPY92_000098"
     CDS             9321..9965
                     /locus_tag="PPY92_000098"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDJ2624130.1"
                     /translation="MTKLYPSLLSVDFLDLQHELKRLEEAGVDGVHFDVMDGQFVPNI
                     SIGLPILDAVRKGTTLPIDVHLMIENPEKYIASFAEHGADMISIHVESTPHIHRAIQM
                     IKHLDKKAGVVINPGTPISQIEPILDIVDYVLVMTVNPGFGGQSFIDQCVEKIAGLNA
                     IKMERQLNFDIEVDGGVNTDTAKVCVENGATMLVTGSFFFKQEDYKKVTQALKG"
     gene            9972..10613
                     /locus_tag="PPY92_000099"
     CDS             9972..10613
                     /locus_tag="PPY92_000099"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDJ2624131.1"
                     /translation="MHINLLCSNRHLPQDIWAKSNEGKWGGVDRGALILLKHQIIPFF
                     SVGDFDSVSKEERQLLTEQLQIKPVQAEKADTDLALAVDKAVALGFDSITIYGATGGR
                     LDHFFGAIQLLLKKAYYKHDVHIEVIDQQNKIELLPKGQHTVEKDKSYPYISFIPMTD
                     DVELSLAGFKYNLARQMLNIGSTLTISNEIESLQAKVTVHDGLILQIRSTDLN"
     gene            complement(10994..11182)
                     /locus_tag="PPY92_000100"
     CDS             complement(10994..11182)
                     /locus_tag="PPY92_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014614133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDJ2624132.1"
                     /translation="MGKQCFVTGRKASTGNRRSHALNSTKRRWNANLQKVRILVDGKP
                     KKVWVSARALKSGKVTRV"
     gene            11625..11999
                     /locus_tag="PPY92_000101"
     CDS             11625..11999
                     /locus_tag="PPY92_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDJ2624133.1"
                     /translation="MTLEISNDYGKIDISNEVIASVVGGKAVECYGIVGMASRQQVRD
                     GIAEILGHENYAKGIKVTENNGVVDIDMYIIVSYGVKISEVANNVQSTVKYTLEKSLN
                     VSVNSINIYVQGVRVNNTGKKA"
     gene            12014..13660
                     /gene="fakA"
                     /locus_tag="PPY92_000102"
     CDS             12014..13660
                     /gene="fakA"
                     /locus_tag="PPY92_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase catalytic subunit FakA"
                     /protein_id="HDJ2624134.1"
                     /translation="MISKINGKLFADMIIQGAQNLSNNADLVDSLNVYPVPDGDTGTN
                     MNLTMTSGREEVENNLSKNIGELGKTFSKGLLMGARGNSGVILSQLFRGFCKNIESES
                     EINSKLLAESFQAGVETAYKAVMKPVEGTILTVAKDAAQAAIEKANNTEDCIELMEYI
                     IVKANESLENTPNLLAVLKEVGVVDSGGKGLLCVYEGFLKALKGEKVEAKVAKIDKDE
                     FVHDEHDFHGVINTEDIIYGYCTEMMVRFGKNKKAFDEQEFRQDMSQFGDSLLVINDE
                     EIVKVHVHTEYPGKVFNYGQQYGELIKLKVENMREQHREVIRKEQHTAKPKMETVETA
                     IITISMGEGISEIFKSMGATHIISGGQTMNPSTEDIVKVIEQSKCKRAIILPNNKNIL
                     MASEQAASIVDAEAVVIPTKSIPQGISALFQYDVDATLEENKAQMADSVNNVKSGSLT
                     YAVRDTKIDGVEIKKDAFMGLIEDKIVSSQSDQLTTVTELLNEMLAEDSEILTVIIGQ
                     DAEQAVTDNMINWIEEQYPDVEVEVHEGGQPIYQYFFSVE"
     gene            13850..15910
                     /gene="recG"
                     /locus_tag="PPY92_000103"
     CDS             13850..15910
                     /gene="recG"
                     /locus_tag="PPY92_000103"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDJ2624135.1"
                     /translation="MAKVNLIESPYSLLQLKGIGPKKIEVLQQLNIHTVEDLVLYLPT
                     RYEDNTVIDLNQAEDQSNVTIEGQVYTAPVVAFFGRNKSKLTVHLMVNNIAVKCIFFN
                     QPYLKKKIELNQTITVKGKWNRVKQEITGNRVFFNSQGTQTQENADVQLEPVYRIKEG
                     IKQKQIRDQIRQALNDVTIHEWLTDELREKYKLETLDFTLNTLHHPKSKEDLLRARRT
                     YAFTELFLFELRMQWLNRLEKSSDEAIEIDYDLDQVKSFIDRLPFELTEAQKSSVNEI
                     FRDLKAPIRMHRLLQGDVGSGKTVVAAICMYALKTAGYQSALMVPTEILAEQHAESLM
                     ALFGDSMNVALLTGSVKGKKRKILLEQLENGTIDCLIGTHALIQDDVIFHNVGLVITD
                     EQHRFGVNQRQLLREKGAMTNVLFMTATPIPRTLAISVFGEMDVSSIKQLPKGRKPII
                     TTWAKHEQYDKVLMQMTSELKKGRQAYVICPLIESSEHLEDVQNVVALYESLQQYYGV
                     SRVGLLHGKLSADEKDEVMQKFSNHEIDVLVSTTVVEVGVNVPNATFMMIYDADRFGL
                     STLHQLRGRVGRSDQQSYCVLIASPKTETGIERMTIMTQTTDGFELSERDLEMRGPGD
                     FFGVKQSGLPDFLVANLVEDYRMLEVARDEAAELIQSGVFFENTYQHLRHFVEENLLH
                     RSFD"
     gene            16113..16685
                     /gene="fapR"
                     /locus_tag="PPY92_000104"
     CDS             16113..16685
                     /gene="fapR"
                     /locus_tag="PPY92_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HDJ2624136.1"
                     /translation="MRGETLKLKKDKRREAIRQQIDSNPFITDHELSDLFQVSIQTIR
                     LDRTYLNIPELRKRIKLVAEKNYDQISSIEEQEFIGDLIQVNPNVKAQSILDITSDSV
                     FHKTGIARGHVLFAQANSLCVALIKQPTVLTHESSIQFIEKVKLNDTVRAEARVVNQT
                     AKHYYVEVKSYVKHTLVFKGNFKMFYDKRG"
     gene            16690..17676
                     /gene="plsX"
                     /locus_tag="PPY92_000105"
     CDS             16690..17676
                     /gene="plsX"
                     /locus_tag="PPY92_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDJ2624137.1"
                     /translation="MVKLAIDMMGGDNAPDIVLEAVQKAVEDFKNLEIMLFGDEKKYN
                     LNHERIEFRHCSEKIEMEDEPVRAIKRKKDSSMVKMAEAVKSGEADGCVSAGNTGALM
                     SVGLFIVGRIKGVARPALVVTLPTIDGKGFVFLDVGANADAKPEHLLQYAQLGDIYAQ
                     KIRGIDNPKISLLNIGTEPAKGNSLTKKSFELLNQDHSLNFVGNIEAKTLMDGDTDVV
                     VTDGYTGNMVLKNLEGTAKSIGKMLKDTIMSSTKNKLAGAILKKDLAEFAKKMDYSEY
                     GGSVLLGLEGTVVKAHGSSNAKAFYSAIRQAKIAGEQNIVQTMKETVGESNE"
     gene            17669..18595
                     /gene="fabD"
                     /locus_tag="PPY92_000106"
     CDS             17669..18595
                     /gene="fabD"
                     /locus_tag="PPY92_000106"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDJ2624138.1"
                     /translation="MSKTAIIFPGQGAQKVGMAQDLFNNNDQATEILTSAAKTLDFDI
                     LETMFTDEEGKLGETENTQPALLTHSSALLAALKNLNPDFTMGHSLGEYSSLVAADVL
                     SFEDAVKIVRKRGQLMAQAFPTGVGSMAAVLGLDFDKVDEICKSLSSDDKIIEPANIN
                     CPGQIVVSGHKALIDELVEKGKSLGAKRVMPLAVSGPFHSSLMKVIEEDFSSYINQFE
                     WRDAKFPVVQNVNAQGETDKEVIKSNMVKQLYSPVQFINSTEWLIDQGVDHFIEIGPG
                     KVLSGLIKKINRDVKLTSIQTLEDVKGWNEND"
     gene            18588..19322
                     /gene="fabG"
                     /locus_tag="PPY92_000107"
     CDS             18588..19322
                     /gene="fabG"
                     /locus_tag="PPY92_000107"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDJ2624139.1"
                     /translation="MTKSALVTGASRGIGRSIALQLAEEGYNVAVNYAGSKEKAEAVV
                     EEIKAKGVDSFAIQANVADADEVKAMIKEVVSQFGSLDVLVNNAGITRDNLLMRMKEQ
                     EWDDVIDTNLKGVFNCIQKATPQMLRQRSGAIINLSSVVGAVGNPGQANYVATKAGVI
                     GLTKSAARELASRGITVNAVAPGFIVSDMTDALSDELKEQMLTQIPLARFGQDTDIAN
                     TVAFLASDKAKYITGQTIHVNGGMYM"
     gene            19757..19990
                     /locus_tag="PPY92_000108"
     CDS             19757..19990
                     /locus_tag="PPY92_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002510960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDJ2624140.1"
                     /translation="MENFDKVKDIIVDRLGVDADKVTEDASFKDDLGADSLDIAELVM
                     ELEDEFGTEIPDEEAEKINTVGDAVKFINSLEK"
     gene            20106..20837
                     /locus_tag="PPY92_000109"
     CDS             20106..20837
                     /locus_tag="PPY92_000109"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDJ2624141.1"
                     /translation="MSKQKKSEIVNRFRKRFDTKMTELGFTYQNIDLYQQAFSHSSFI
                     NDFNMNRLDHNERLEFLGDAVLELTVSRYLFDKHPNLPEGNLTKMRATIVCEPSLVIF
                     ANKIGLNEMILLGKGEEKTGGRTRPSLISDAFEAFIGALYLDQGLDIVWKFAEKVIFP
                     HVEQNELLGVVDFKTQFQEYVHQQNKGDVTYNLIKEEGPAHHRLFTSEVILQGEAIAE
                     GKGKTKKESEQRAAESAYKQLKQIK"
     gene            20984..24550
                     /gene="smc"
                     /locus_tag="PPY92_000110"
     CDS             20984..24550
                     /gene="smc"
                     /locus_tag="PPY92_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDJ2624142.1"
                     /translation="MVYLKSIDAIGFKSFADQTNVQFDKGVTAIVGPNGSGKSNITDA
                     IKWVLGEQSAKSLRGSKMEDIIFSGAEHRKAQNYAEVQLRLDNHSKKLSVDENEVIVT
                     RRLYRSGESEYYINNDRARLKDIADLFLDSGLGKEAYSIISQGRVDEILNAKPIDRRQ
                     IIEESAGVLKYKKRKAESLNKLDQTEDNLTRVEDILYDLEGRVEPLKEEAAIAKEYKT
                     LSHQMKHSDIVVTVHDIDQYTNDNRQLDQRLNDLQGQQANKEADKQRLSQQIQQYKGK
                     RHQLDNDVESLNYQLVKATEAFEKYTGQLNVLEERKKNQSETNARYEEEQENLMELLE
                     NISNEISEAQDTYKSLKSKQKELNAVIRELEEQLYVSDEAHDEKLEEIKNEYYTLMSE
                     QSDVNNDIRFLKHTIEENEAKKSRLDSRLVEVFEQLKDIQGQIKTTKKEYQQTNKELS
                     AVDKEIKNIEKDLTDTKKAQNEYEEKLYQAYRYTEKMKTRIDSLATQEEEYTYFFNGV
                     KHILKAKNKELKGIHGAVAEIIDVPSKLTQAIETALGASLQHVIVDSEKDGRQAIQFL
                     KERNLGRATFLPLNVIQSRVVATDIKSIAKEANGFISIASEAVKVAPEYQNIIGNLLG
                     NTIIVDHLKHANELARAIKYRTRIVTLEGDIVNPGGSMTGGGARKSKSILSQKDELTT
                     MRHQLEDYLRQTESFEQQFKELKIKSDQLSELYFEKSQKHNTLKEQVHHFEMELDRLT
                     TQETQIKNDHEEFEFEKNDGYTSDKSRQTLSEKETHLESIKASLKRLEDEIERYTKLS
                     KEGKESVTKTQQTLHQKQSDLAVVKERIKTQQQTIDRLNNQSQQTKHQLKDVKEKIAF
                     FNSDEVMGEQAFQNIKDQINGQQETRTRLSDELDKLKQQRIELNEQIDAQEATLQVCH
                     QDILAIENHYQDIKAEQSKLDVLIHHAIDHLNDEYQLTVERAKSEYTSDESIDALRKK
                     VKLMKMSIDELGPVNLNAIEQFEELNERYTFLSEQRTDLRKAKETLEQIISEMDQEVT
                     ERFKETFHAIQGHFTAVFKQLFGGGDAELQLTEADYLTAGIDIVVQPPGKKLQHLSLL
                     SGGERALTAIALLFAILKVRSAPFVILDEVEAALDEANVIRYAKYLNELSDETQFIVI
                     THRKGTMEFADRLYGVTMQESGVTKLVSVNLNTIDDVLKEEQ"
     gene            24550..25800
                     /gene="ftsY"
                     /locus_tag="PPY92_000111"
     CDS             24550..25800
                     /gene="ftsY"
                     /locus_tag="PPY92_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDJ2624143.1"
                     /translation="MSFFKRLKDKFATNKENEEVKSLREEQGQDKLEDTHSEGSTQDV
                     NDLAENAEVKKKPRKLSEADFDDDGLISIEDFEEIEAQKMGAKFKAGLEKSRQNFQEQ
                     LNNLIARYRKVDEDFFEALEEMLITADVGFNTVMTLTEELRMEAQRRNIQDTEDLREV
                     IVEKIVEIYHQEDDNSEAMNIEDGRLNVILMVGVNGVGKTTTIGKLAYRYKMEGKKVM
                     LAAGDTFRAGAIDQLKVWGERVGVDVISQSEGSDPAAVMYDAINAAKNKGVDILICDT
                     AGRLQNKTNLMQELEKVKRVINRAVPDAPHEALLCLDATTGQNALSQARNFKEVTNVT
                     GIVLTKLDGTAKGGIVLAIRNELHIPVKYVGLGEQLDDLQPFNPESYVYGLFADMIEQ
                     NDEITTVENDQIVTEEKDDNHGAK"
     gene            25787..26119
                     /locus_tag="PPY92_000112"
     CDS             25787..26119
                     /locus_tag="PPY92_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDJ2624144.1"
                     /translation="MGQNDLVKTLRMNYLFDFYQSLLTNKQRNYLELFYLEDYSLSEI
                     ADTFNVSRQAVYDNIRRTGDLVEDYEKKLELYQKFEQRREIYDEMKQHLSNPEQIQRY
                     IQQLEDLE"
     gene            26145..27512
                     /gene="ffh"
                     /locus_tag="PPY92_000113"
     CDS             26145..27512
                     /gene="ffh"
                     /locus_tag="PPY92_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002457378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDJ2624145.1"
                     /translation="MAFEGLSERLQATMQKMRGKGKLTEADIKIMMREVRLALFEADV
                     NFKVVKEFIKTVSERALGSDVMQSLTPGQQVIKIVQDELTKLMGGENTSINMSNKPPT
                     VVMMVGLQGAGKTTTAGKLALLMRKKYNKKPMLVAADIYRPAAINQLQTVGKQIDIPV
                     YSEGDQVKPQQIVTNALKHAKEEHLDFVIIDTAGRLHIDEALMNELKEVKEIAKPNEI
                     MLVVDSMTGQDAVNVAESFDDQLDVTGVTLTKLDGDTRGGAALSIRSVTQKPIKFVGM
                     SEKLDGLELFHPERMASRILGMGDVLSLIEKAQQDVDQEKAKDLEKKMRESSFTLDDF
                     LEQLDQVKNLGPLDDIMKMIPGMNKMKGLDKLNMSEKQIDHIKAIIQSMTPAERNNPD
                     TLNVSRKKRIAKGSGRSLQEVNRLMKQFNDMKKMMKQFTGGGKGKKGKRNQMQNMLKG
                     MNLPF"
     gene            27947..28222
                     /gene="rpsP"
                     /locus_tag="PPY92_000114"
     CDS             27947..28222
                     /gene="rpsP"
                     /locus_tag="PPY92_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDJ2624146.1"
                     /translation="MAVKIRLTRLGSKRNPFYRIVVADARSPRDGRIIEQIGTYNPTS
                     ANAPEIKVDEALALKWLNDGAKPTDTVHNILSKEGIMKKFDEQKKAK"
     gene            28410..28913
                     /gene="rimM"
                     /locus_tag="PPY92_000115"
     CDS             28410..28913
                     /gene="rimM"
                     /locus_tag="PPY92_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDJ2624147.1"
                     /translation="MRVEVGQIVNTHGIKGEIKVKSNSDFTDVRFQPGQVLTVVHNNN
                     DLEYTVKSHRVHKGLHMLTFEGINNINDIEHLKGSSIYQERDHEDIVLEENEFYYSDI
                     IGCTVFDDQETPIGRVINIFETGANDVWVIKGSKEYLIPYIADVVKEVDVENKKIIIT
                     PMEGLLD"
     gene            28913..29650
                     /gene="trmD"
                     /locus_tag="PPY92_000116"
     CDS             28913..29650
                     /gene="trmD"
                     /locus_tag="PPY92_000116"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDJ2624148.1"
                     /translation="MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAIN
                     KHNQVDDYPYGGGQGMVLKPEPVFNAMEDLDVTEQTRVILMCPQGEPFSHQKAVELSK
                     ADHIVFICGHYEGYDERIRTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGN
                     EQSHQDDSFSDGLLEFPQYTRPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKR
                     PDLIEKYPLTNEDKQILERYKIGLKKG"
     gene            29753..30103
                     /gene="rplS"
                     /locus_tag="PPY92_000117"
     CDS             29753..30103
                     /gene="rplS"
                     /locus_tag="PPY92_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDJ2624149.1"
                     /translation="MTNHKLIEAVTKSQLRTDLPSFRPGDTLRVHVRIIEGTRERIQV
                     FEGIVIKRRGGGVSETFTVRKISSGVGVERTFPLHTPKIEKIEVKRRGKVRRAKLYYL
                     RSLRGKAARIQEIR"
     gene            complement(30347..32953)
                     /gene="yfhO"
                     /locus_tag="PPY92_000118"
     CDS             complement(30347..32953)
                     /gene="yfhO"
                     /locus_tag="PPY92_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosyltransferase
                     YfhO"
                     /protein_id="HDJ2624150.1"
                     /translation="MLKKWLNSNVKQFFVITFISVILTLILFSTHIYDYIVNGTVFSG
                     AGDGFRQMMPFQMYLYEHLSSFSSLYDASFGLGGDYMKGLSYYYSLSPLMWLNFLFIK
                     IGETVGIFNPTTIHFWPTNQLIMAMIRAIITFVVTFYLFKILHFKRSTNMIATILYGM
                     STVVIYFNFTWSFYGNLLYLLPLSILGLERYFQQRKIGIFIVAIALTLFSNFYFSYYQ
                     AIIIGCYYLYRLIFTYKYDIVSRTQKFICVISATVLSVLSSVFGLFTGISAFLENDRK
                     QNPNVDIPFLTPLDYHYFFFSDGFYITISILTIVALLSFKLYRFYFYRLFAIVTWILF
                     IGSLSQYFDSAFNGFSFPERRWVYILALSSSALCGLFIQHLSTLNMKYYLIRTIPVSI
                     IALLYVLLSPTHPLALIVGIILLMVLAVILKFSLWRYKKLTVAILVLIVMIQQIVILD
                     NNKNMAIKPYQQSLSTLKQHDYHSNYVNQLIKKINQNATGPFNRIDYMSDYALNSPFI
                     YHYNGISLYSSIFNGDILKYYDKTLQINMPIDKNSTYRLLGNRQNLLSLWNVNDRIRV
                     NHDDNLPYRFKINSEHKDNKVRWIHSKNTIHYPSAHITNKVFSNKELKSPLDKEQAML
                     QGIVSNNTKDVNTHFKANKNLLSDSTIKLNSSAWQSPTKHLLQVKQNNGGLTVQLPKS
                     VSNQFKDLYFEMDLELLSPDKAHDVKVNEYTQERNKLTYKYRRFVTPVTIRIKASDRI
                     RLSLPKGKYRVNLKGIYGEDYTTLKDASNSLEAVKVSKTKQGYTITKNKNSSGYIVLP
                     TAYNQGMKATSGDQSLKVAQVNGVMTGIKAPKNITKIQLSYTPPYYYLLITITIFGII
                     CSIIFTRWARQK"
     gene            33354..34238
                     /gene="ylqF"
                     /locus_tag="PPY92_000119"
     CDS             33354..34238
                     /gene="ylqF"
                     /locus_tag="PPY92_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDJ2624151.1"
                     /translation="MVIQWYPGHMAKAKREVSEQLKKVDVVFELVDARIPYSSRNPMI
                     DEVINQKPRVVILNKKDMSNLNEMSKWEQFFIDKGYYPVSVDAKHGKNLKKVEAAAIK
                     ATAEKFEREKAKGLKPRAIRAMIVGIPNVGKSTLINKLAKRSIAQTGNKPGVTKQQQW
                     IKVSNALQLLDTPGILWPKFEDEEVGKKLSLTGAIKDSIVHLDEVAIYGLNFLIQNDL
                     ARLKSHYNIEVPEDAEIIAWFDAIGKKRGLIRRGNEIDYEAVIELIIYDIRNAKIGNY
                     CFDIFKDMTEELANDANN"
     gene            34222..34989
                     /locus_tag="PPY92_000120"
     CDS             34222..34989
                     /locus_tag="PPY92_000120"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDJ2624152.1"
                     /translation="MTLTIKEVTQLINAVNTIEELENHECFLDERKGVQNAIARRRKA
                     LEKEQALKEKYVEMTYFENEILKEHPNAIICGIDEVGRGPLAGPVVACATILNSNHNY
                     LGLDDSKKVPITKRLELNEALKNEVTAFAYGIATAEEIDEFNIYKATQIAMQRAIDGL
                     SVQPTHLLIDAMTLDNALPQVSLIKGDARSVSIAAASIMAKVFRDDYMTQLSKDYPEY
                     GFEKNAGYGTKQHLLAIDDIGIMKEHRKSFEPIKSLL"
     gene            35098..36264
                     /gene="sucC"
                     /locus_tag="PPY92_000121"
     CDS             35098..36264
                     /gene="sucC"
                     /locus_tag="PPY92_000121"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDJ2624153.1"
                     /translation="MNIHEYQGKEIFRSMGVAVPEGRVAFTAEEAVEKAKELNSDVYV
                     VKAQIHAGGRGKAGGVKIAKSLSEVETYAKELLGKTLVTHQTGPEGKEIKRLYIEEGC
                     AIQKEYYVGFVIDRATDQVTLMASEEGGTEIEEVAAKTPEKIFKETIDPVIGLSPFQA
                     RRIAFNINIPKESVNKAAKFLLALYNVFIEKDCSIVEINPLVTTADGDVLALDAKINF
                     DDNALFRHKDVVELRDLEEEDPKEIEASKHDLSYIALDGDIGCMVNGAGLAMATMDTI
                     NHFGGNPANFLDAGGSATREKVTEAFKIILGDENVKGIFVNIFGGIMKCDVIAEGIVE
                     AVKEVDLTLPLVVRLEGTNVELGKKILKDSGLAIEPAATMAEGAQKIVKLVKEA"
     gene            36286..37194
                     /gene="sucD"
                     /locus_tag="PPY92_000122"
     CDS             36286..37194
                     /gene="sucD"
                     /locus_tag="PPY92_000122"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDJ2624154.1"
                     /translation="MSVFIDKNTKVMVQGITGSTALFHTKQMLDYGTKIVAGVTPGKG
                     GQVVEGVPVFNTVEEAKNETGATVSVIYVPAPFAADSILEAADADLDMVICITEHIPV
                     LDMVKVKRYLQGRKTRLVGPNCPGVITADECKIGIMPGYIHKKGHVGVVSRSGTLTYE
                     AVHQLTEEGIGQTTAVGIGGDPVNGTNFIDVLKAFNEDDETKAVVMIGEIGGTAEEEA
                     AEWIKANMTKPVVGFIGGQIAPPGKRMGHAGAIISGGKGTAEEKIKTLNSCGVKTAAT
                     PSEIGSTLIEAAKEAGIYESLLTVNK"
BASE COUNT        13859 a         5027 c         7215 g        11195 t
ORIGIN      
        1 ttatttgatt caaacaaaat aacttaagag gagaaagttt atgaaaaaga cactgggatg
       61 tttactttta attatgcttt tagtcgtagc aggttgttct tttggtggga atcataaatt
      121 atcatcaaag aaatcagaag aatcaaaaca agaaactgta aaaaaagaat cggaagaaga
      181 gaaagatcca gatttagaga aatatgaaga aatagagaag aaaatgaaag gaattaaaga
      241 tgcgccatct cttgataagt tggatccatt aatgacagaa aagtcgttta cgaatagtaa
      301 agggattcaa ggatggaaag attacaaaga attaatgggt aaagtggaac ttgcagatta
      361 tagatttact aaagattcaa aaggatcttc aataaaagat gttgatgcat tctttaaagg
      421 taagaaaggt ataaaaagga aagtgattga aacacacgat gatgtaaaac aagttgatta
      481 ttggtatgta gatccagatg gaaagaaaat tggcaattca aacacacctg ttttttacgc
      541 agaaattatg acaaaatata aagatggaaa gttagtttat gcatcagtcg aaccaggatc
      601 ttacgtaata cataaagatg atgcaattaa atatgacgat tattctaagt taaaaaaatt
      661 aagtcagcta actaaacttg atcatccaaa accagttcca tatagcgtag ctcaaatcaa
      721 atctttcgga gtacctttaa caagcgtttc atttatgaca catggatcaa aggatactaa
      781 agatgaagtg ttgccggcat tggcctattt cactttttca ccaaaaaatt atgaagacaa
      841 gtctaatcca gatccaaaag ttttaaattt agtacatatg gatttcttaa atgcatctag
      901 tgattttggt aacgcacatt ttgttgtttt aagtaaatat attaaagagt atgaatcaaa
      961 ctatgaaaca gcgtcagatg attctttaaa atagtattta ctgtgtgaaa aataaatagt
     1021 gtactacatt aaataatcgc aataataatc ccgataaaca atcagcatta ctgcttatca
     1081 tatagagttc gtaataacta taactctatg attcgcaaat aataaatgat tgtcatcggg
     1141 atttattttt atcaatttat aaagtgacat taccttgttc atcagcaggt ttgaaaacag
     1201 taatcactgc actaataatt gctaaaatgt gtgggatacc tgtccaacag aatattaagt
     1261 gtagaatacc ttgcatattc ttgccggcat aaaatttatg aataccaaaa ctacctaaga
     1321 acaatgctaa taaaatataa ataactttgt ttacttgcat ttctttccct ccagttgaat
     1381 tgcttataat gtcattagct tctcttttta ttatacccac ttttagttca aacattctag
     1441 tttaagcatt cccaatcatc taaatttcag ttattcattc cttacaataa atttaggatt
     1501 acacttcagt tgcattgtat tattttacgt gtgaaatata cgtaatgaat cacatgacaa
     1561 tcttcaaatt gaaaaatata cattctatga tgtaaggtcg catttttaat atatttacgt
     1621 tataatagtt tgatgaattt tattgaggag taagagttat ggcgattaaa aagttagtac
     1681 cagcatcgca tcctatttta acgaaaaaag cgcaagcagt taaaacattt gatgattcgt
     1741 taaaaagatt attacaagat ttagaagata caatgtatgc acaagaagct gctggcttat
     1801 gtgcacctca aattaatcag tcattgcaag tggcaattat tgatatggaa atggaaggat
     1861 tattacaact tgttaatccg aaaattatta gtcaatcaaa tgaaacaata acagacttag
     1921 aaggttcaat tacattgcca gatgtttacg gcgaagtgac aagaagtaaa atgatagttg
     1981 ttgaaagtta tgacgtcaat gggaacaaag ttgaactaac tgcacatgaa gatgtagcaa
     2041 gaatgatttt gcatattata gatcaaatga acggtatccc ttttacagaa cgtgcggacc
     2101 gtattttaac agataaagaa gtggaggcat attttataaa tgactaaaat aatatttatg
     2161 ggtacaccag acttttcaac aactgtttta gaaatgctta ttgcagaaca tgatgtcatt
     2221 gcagtcgtaa cgcaaccaga tcgacctgtt ggacgtaaac gtgttatgac accaccacca
     2281 gttaaaaaag ttgcaatgaa atatgattta cctgtatatc agcctgaaaa attaagtgga
     2341 tcagaagaat tagaacaatt gcttcaatta gatgtagatt taattgtaac tgctgctttt
     2401 ggacaattat tacctgaatc attgttggca ttaccaaaac ttggcgcaat taatgtacat
     2461 gcatcattgt taccaaagta tagaggtggt gcaccaattc atcaggcaat tatcgatggt
     2521 gaacaagaaa ccggcataac aattatgtat atggttaaaa aattagatgc gggtaatatt
     2581 atttcgcaac aagcaattaa aatagaagaa aatgataatg tcggtacgat gcatgataaa
     2641 ttaagtgtat taggggcaga tttattaaaa gaaactttac catctattat agagggcaca
     2701 aatgaaagtg tacctcaaga tgatacgcaa gcaacatttg cttccaatat tcgacgcgaa
     2761 gatgagcgaa ttagctggaa taaaccagga agacaagtgt ttaatcaaat tcgtggatta
     2821 tcaccatggc cagttgctta tacaactatg gatgacacta acttgaaaat atacgatgct
     2881 gaactcgttg agactaataa gataaacgag cctggaacca ttatagaaac gactaaaaaa
     2941 gccattattg ttgctacaaa tgataatgaa gctgttgcaa ttaaagatat gcaattagct
     3001 gggaaaaaga gaatgttagc tgccaattat ttaagtggtg cgcaaaacac actagtaggg
     3061 aagaaactta tatgatcgaa aacgtgagaa gtcttgcttt tgacacgatt caagatatat
     3121 taaatgaagg tgcgtatagt aacttgcgta tcaatgaagt gttgtcagaa aatgaattaa
     3181 atgcaatgga taaggcttta tttacagaaa ttgtctacgg aaccgttaaa agaaaatata
     3241 cgttagattt ttatttaaag ccttttgtga aaacaaagat taaggcatgg gttaggcaat
     3301 tattatggat gagtatttat caatatgtat atttagataa agttccaaat catgccatta
     3361 ttaatgaagc agttgaaata gcaaaagaac gcggtggcta tcataatggt aatgtcgtaa
     3421 atggtatttt acgtacaatg atgcgtagtg acttacctga ttttaatgaa attgcagatc
     3481 ctaaaaaaag aatggcaatc gagtatagta tgccgaagtg gattatagat cattgggcaa
     3541 cacattatgg tctcgaagaa actgaaacaa ttttacagtc atttttagaa acgacatcaa
     3601 caactgtgcg tgccaacctg acgcgagcat cattagatga tattattgaa aagttgcaag
     3661 acgaaggtta tgacgttgaa aaagatcatg acttacctta ttgtctccat ataggaggac
     3721 aaccaattat tcattctcgt tcatttaaag atggattcgt ttcaattcaa gataaaagct
     3781 caatgtttgt tgcacacatt atgaatgtag accgacatga tcacgtatta gatgcatgta
     3841 gtgcacctgg cggtaaagct tgtcacattg ctgaagtttt aatgccagaa gggcaagttg
     3901 acgcttcaga tatacatgat cacaaaatag acttaattaa ttttaatata aaaaaattac
     3961 gattaacaaa tattaaggct tttcaacatg atgcgacaaa accttatgat aaaacatacg
     4021 ataagatact tgttgatgca ccatgtagcg gattaggtgt aatgagacat aagccggaga
     4081 ttaagtatac tcaaagcaaa caacatattg agtcactagt tgaattacag cttgaaatat
     4141 tggaaaatgt aaaaaacaat gtaaaaatag gtggcgaaat catctattca acatgtacaa
     4201 ttgagcaact agaaaatgaa aacgtgattt atacgtttct gaaaaataat aaaaacttcg
     4261 aatttgaacc gtttcaacat ccgataactg gagagttggt caaaacgtta caaatcatgc
     4321 cgcaagactt taattcagat ggattcttta tcactaagat aaaaagaaag gacaattagg
     4381 aatgataact gctgaaaaga aaaagaagaa taaatttctt ccaaattttg acaagcaatc
     4441 aatatattca ttgcgatttg acgaaatgca aaactggctc gttgaacaag gtcaacaaaa
     4501 atttcgagcg aaacagattt ttgaatggtt atatcaaaaa agagtagatt cgattgatga
     4561 aatgacgaac ttatcgaaag acttacgaca gcttttaaaa gataacttta ctgttacaac
     4621 tttaacaact gtagtaaaac aagaaagtaa agacggtaca attaaattct tatttgaatt
     4681 acaagatggc tatacaattg aaactgtttt aatgagacat gattatggaa attcagtatg
     4741 tgtaacgaca caagtaggtt gtcgtatcgg atgtacgttt tgtgcttcta cacttggcgg
     4801 cttaaaaaga aaccttgaag ctggcgaaat tgtttcacaa gttttaacag ttcaaaaagc
     4861 ccttgatgct acagaagagc gcgtatctca aattgtcata atgggtatcg gtgaaccatt
     4921 tgaaaattat gatgaaatga tggacttttt aagaatcgtc aatgatgata acagtttaaa
     4981 tattggtgca cgtcacatta cagtatcaac atcaggtatc attcctagaa tatatgactt
     5041 tgcggatgaa gatatccaaa ttaattttgc tgtaagctta cacgccgcaa aagatgaagt
     5101 gcgatcacgc ttgatgccaa ttaaccgtgc atataatgtt gagaagttaa tcgaagcaat
     5161 tcaatattat caagaaaaaa caaatcgtcg tgttactttt gaatatggtc tgtttggtgg
     5221 tgtgaatgac caactagaac atgcaagaga attagcacat ttaataaaag gcttaaactg
     5281 ccatgttaac ttaattcctg tcaaccatgt tccagaaaga aattatgtga aaacggctaa
     5341 aaatgatatc tttaaatttg aaaaagaatt aaagagacta ggaattaatg ccacaatacg
     5401 tcgtgaacaa ggttcggata ttgacgcagc ttgtggtcaa ttaagagcaa aggaacgaca
     5461 agtagaaacg aggtaaagac aaatgctaga ggcacaattt tttactgata ctggacaaca
     5521 tagagataag aatgaagatg cgggtggtat tttttataat caaactaatc aacaactttt
     5581 agttctgtgt gatggtatgg gcggccataa agcaggagaa gttgcaagta aatttgttac
     5641 agatgagttg aaatcccgtt ttgaagcgga aaatcttata gaagaacatc aagctgaaaa
     5701 ttggttgcgt aataatataa aagatataaa ttttcagtta tatcactatg cacaagaaaa
     5761 tgcagaatat aaaggtatgg gtacaacatg tgtttgtgca cttgtttttg aaaaatcagt
     5821 tgtgatagca aatgtcggtg attctagagc ctatgttatt aatagtagac aaattgaaca
     5881 aattactagt gatcactcat ttgttaatca tcttgtttta acgggtcaaa ttacgccgga
     5941 agaagcattt acacatccac aacgtaatat tattacgaag gtgatgggca cagataaacg
     6001 tgtgagtcca gatttgttta ttaagcgatt aaatttttat gattatttat tattaaattc
     6061 agatggatta actgattatg ttaaagacaa tgaaattaag cgtttgttag taaaagaagg
     6121 tacaatagaa gatcatggtg atcaattaat gcaattggca ttagataacc attcgaaaga
     6181 taacgttact ttcatactcg cggctattga aggtgataaa gtatgatagg taaaataata
     6241 aatgaacgat ataaaattgt agataagctt ggcggcggtg gcatgagtac cgtttatctt
     6301 gcagaagata cgatacttaa cattaaagtt gcaattaagg cgatttttat accacctaga
     6361 gaaaaagaag aaacattaaa acgttttgaa cgagaagtac ataactcatc acagctatca
     6421 catcaaaata tagtaagtat gatcgatgtt gatgaagaag atgactgtta ctacttagta
     6481 atggaatata ttgaaggtcc gactttgtct gagtatattg aaagtcatgg gccattaagt
     6541 gttgacacgg cgattaattt tacgaatcaa atattggatg gcattaaaca tgcgcatgat
     6601 atgcgtattg tacatagaga tattaagcca caaaatatat taattgacag caataaaacg
     6661 ttgaaaatat ttgattttgg aattgctaaa gctttaagtg agacgtcttt aactcagact
     6721 aatcatgtgt taggtactgt gcagtacttt tcgccagaac aagcaaaagg tgaggcaacg
     6781 gatgaatgta cagatattta ttctataggt attgtgttat atgaaatgct tgttggtgaa
     6841 ccacccttta atggagaaac tgcagttagc attgcgatta aacatattca ggattctgtg
     6901 ccaaatgtga caacagatgt acgtaaggat attccgcaat ctttaagtaa tgtcatttta
     6961 cgcgctacag aaaaagacaa agcgaatcgt tacaaaacaa ttcaagaaat gaaagatgat
     7021 ttgagtagtg ttttacatga aaatcgagcg aatgaagatg tctatgaact cgataaaatg
     7081 aaaacgatag cggtaccttt gaaaaaagaa gatctagcaa agcatattag tgaacataag
     7141 tcgaatcaac ctaaacgtga aacgacgcaa gtacctattg taaatgggcc tgctcatcat
     7201 cagcaattcc aaaagccaga aggtacggtg tacgaaccaa aacctaaaaa gaaatcaaca
     7261 cgaaagattg tgctcttatc actaatcttt tcgttgttaa tgattgcact tgtttctttt
     7321 gtggcaatgg caatgtttgg taataaatac gaagagacac ctgatgtaat cgggaaatct
     7381 gtaaaagaag cagagcaaat attcaataaa aacaacctga aattgggtaa aatttctaga
     7441 agttatagtg ataaatatcc tgaaaatgaa attattaaga caactcctaa tactggtgaa
     7501 cgtgttgaac gtggtgacag tgttgatgtt gttatatcaa agggccctga aaaggttaaa
     7561 atgccaaatg tcattggttt acctaaggag gaagcattgc agaaattaaa atcgttaggt
     7621 cttaaagatg ttacgattga aaaagtatat aataatcaag cgccaaaagg atacattgca
     7681 aatcaaagtg taaccgcaaa tactgaaatc gctattcatg attctaatat taaactatat
     7741 gaatctttag gcattaagca agtttatgta gaagactttg agcataaatc ctttagcaaa
     7801 gctaaaaaag ccttagaaga aaaagggttt aaagttgaaa gtaaggaaga gtatagtgac
     7861 gatattgatg agggtgatgt gatttctcaa tctcctaaag gaaaatcagt agatgagggg
     7921 tcaacgattt catttgttgt ttctaaaggt aaaaaaagtg actcatcaga tgtcaaaacg
     7981 acaactgaat cggtagatgt accatacact ggtaaaaatg ataagtcaca aaaagttaaa
     8041 gtttatatta aagataaaga taatgacggt tcaactgaaa aaggtagttt cgacattact
     8101 agtgatcaac gtatagacat tcctttaaga attgaaaaag gaaaaacagc aagttatatt
     8161 gttaaagttg acggtaaaac tgtagctgaa aaagaagtca gctatgatga tatttaaata
     8221 taattgaagt aaatgtaccg aggtttctat ttggaagtct cggtattttt atgttgagat
     8281 tgcggtagtt ttaaaatgct taatgtcatc atttacagtt gagcattgtc tatcaattct
     8341 gtatcactct cacaatattt atatcatttg aaatgatcat ttatatatta attttccagt
     8401 ctttgatata ataaatgcag taaaattaat cgagaggtgc cattttgaag acaggtcgaa
     8461 tagtgaaatc aattagtggg gtatatcaag tagacgttaa tggcgaacgt ttcaatacaa
     8521 aaccacgagg attatttaga aagaaaaaat tttcaccggt agttggtgat atagtggaat
     8581 ttgaagtaca aaacattaac gaaggctata ttcatcaagt gtttgagcgg aaaaatgagt
     8641 tgaaaagacc acctgtaagt aatatagata cactagtaat tgtaatgagt gctgtcgagc
     8701 caaatttttc aacgcaatta ttagatcgat ttttagttat tgcacattcg tatcagttaa
     8761 atgcgagagt tttggtgact aaaaaagata aaacaccaat tgaaaagcag ttcgaaatta
     8821 atgagttgtt gaaaatatat gaaaatattg gctatgagac tgaatttatt ggaaatgatg
     8881 atgatcgaaa aaaaattgta gaagcttggc cagctggact tatagtactt agtggtcaat
     8941 caggtgtcgg taagtccact ttcttaaatc attatcgtcc agaacttaat cttgagacaa
     9001 atgatatatc aaaatcatta aatcgaggaa agcatactac aagacatgtc gaactattcg
     9061 aacgtcaaaa cggttatatt gcagacacac ctggattcag tgctttagat tttgatcata
     9121 tagataaaga tgaaataaaa gattattttc ttgaattaaa tcgatatggt gaaacatgta
     9181 agtttaggaa ttgtaatcat atcaaagaac ctaattgtaa tgttaagcat caattagaga
     9241 tagggaatat tgcgcaattt agatacgacc attatttaca actatttaat gaaatttcaa
     9301 atagaaaggt tagatattaa atgacaaaac tatatccatc attattatct gttgattttt
     9361 tggatttaca acatgaatta aaacgacttg aagaagcagg tgtcgacgga gttcattttg
     9421 atgttatgga tggtcaattt gtgcctaata tatctattgg tttaccaata ttagatgcag
     9481 taagaaaagg cacaacatta cctatagacg tacatttgat gattgaaaat ccagaaaagt
     9541 atattgcatc atttgcagaa catggtgccg atatgatttc aattcatgtc gaatcaacgc
     9601 ctcatattca tcgtgctatt caaatgatta aacatttaga taaaaaagct ggtgtagtaa
     9661 ttaatcctgg tacaccaata tcacaaattg aacctatttt agacattgtt gattatgtat
     9721 tagtgatgac agttaaccca gggtttggtg gtcaatcatt tattgatcaa tgcgtagaaa
     9781 aaatagcggg tcttaatgct attaaaatgg aacgtcaatt aaactttgat attgaagttg
     9841 atggaggcgt aaataccgat acagcgaaag tttgtgttga aaatggtgct acaatgctag
     9901 taacaggttc atttttcttt aaacaagaag attataaaaa agtcacacaa gcattgaaag
     9961 gttgagtgta tatgcatata aatttattat gttctaatcg acacttgccg caagatattt
    10021 gggccaaaag taatgaaggt aaatggggcg gcgttgatag aggtgctttg attttattga
    10081 agcatcaaat tatccctttt ttctcagtgg gagactttga ttcagtcagt aaagaagaac
    10141 gccaacttct aacagaacag ttacaaatca aaccagttca agctgaaaaa gctgatacgg
    10201 atttagcttt agcggttgat aaagctgttg cacttggatt tgatagtatt acaatttatg
    10261 gtgcaacagg cggacgatta gatcactttt ttggggcaat tcagttatta ttgaaaaaag
    10321 catattataa acatgatgtt catatagaag ttatcgatca acaaaataaa attgaattat
    10381 tgcctaaagg tcaacataca gttgaaaaag ataagagtta tccgtacatt tcatttatac
    10441 cgatgactga tgatgtagaa ctttctctag caggttttaa atataattta gctagacaaa
    10501 tgcttaatat aggttctact ttaactattt caaatgaaat tgagtctttg caagcgaaag
    10561 taactgtaca tgatgggttg attttgcaaa ttagaagtac agatttaaat taagtatatt
    10621 caaatttgtg aggattttta ataaagtgca tgtgttattt aataaagtga cgtttataga
    10681 tagaatttat aacataataa tagatgaatc acgctgtaat taatgaagat tatggcgtga
    10741 tttattattg gaaggaaaat tgtaggtcct agtacattga tttatgtgcc aaaatcgata
    10801 ttgaacccat aagtccaatt tgacagagaa ccattattaa acgtattaac caaattatat
    10861 tgatagttcg aatttcacta tgaaccatta tgtattgttt ggtaacaaaa aaagaactaa
    10921 gaggttgagc tcttagttct taagtaaatg tgtcctccac ataaggtcac attttttatg
    10981 tgcaatatat ttattaaact ctagttactt taccagattt taaagcacgt gcagaaaccc
    11041 aaactttttt aggtttaccg tcaactagga ttctaacttt ttgaaggtta gcgttccatc
    11101 tacgtttagt agagtttaaa gcgtgtgaac gtctgttacc agtcgaagct ttacgacctg
    11161 ttacgaaaca ttgtttaccc atatgagtac ctcctttaat aaatataaat acacataact
    11221 acttatatac ttaattaaga tagcatagtt tcatttgaaa aacaatgaat aattttcaca
    11281 taaaagtcaa aaatactgag ttttgtgata taattgtaga ctgtgaagtt atgtagtatg
    11341 atattttaga gaaataagtg aatgaaacat ttaaaatttt tatttaatga tactacatct
    11401 attaaaagaa aacgcctata gataatcgtc atgagtgtaa attcagttta ctcaaaatat
    11461 aataggtgat ttaagcgtaa gtcattctga taagttgtac cattgatatt ttataaaaat
    11521 aatcaatgaa acttaaagcg tttatgacta cactaagtgg taaaatttta aaagtgatta
    11581 gggaaaacga ataataaact ttgaaattta ggagggacaa agatatgaca ttagagattt
    11641 caaatgatta cggtaaaatt gatatttcaa acgaagtgat tgcttcggtt gtaggtggaa
    11701 aggccgttga atgttatggt attgtaggta tggcatctag acaacaagtt agagatggta
    11761 ttgcggaaat actaggacat gaaaattatg ctaaaggcat caaagtaact gaaaataatg
    11821 gcgtagtgga tatagatatg tacattattg taagttatgg tgtgaaaata tctgaagttg
    11881 ccaataatgt acaatcaaca gtgaaatata ctttggaaaa atcacttaat gtatcagtaa
    11941 attcaatcaa tatatatgta caaggtgtac gtgtgaataa tacaggcaag aaagcttagg
    12001 aggacaactt gaaatgatta gcaaaattaa tggtaaatta tttgccgata tgattataca
    12061 aggggcacaa aatttatcta acaatgcaga tttggtagat tctttgaatg tgtatccagt
    12121 gccagatggt gatacaggaa caaatatgaa tcttactatg acttcaggtc gcgaagaagt
    12181 agagaataat ttgtcgaaaa atatcggcga attaggtaaa acattctcga aaggtttact
    12241 aatgggtgca agaggtaact ctggtgtcat cttgtcacaa ttattcagag gattttgtaa
    12301 aaatattgaa agtgaatctg aaattaattc aaaattgtta gctgaaagtt ttcaagctgg
    12361 tgttgaaacg gcatataaag ctgttatgaa accagttgaa ggtacaatac ttacagttgc
    12421 aaaagatgct gcgcaagctg caatagaaaa agcaaataat actgaagatt gtatagaatt
    12481 aatggagtac attattgtaa aagccaatga atcacttgaa aacacaccaa acttattagc
    12541 tgtacttaaa gaagttggtg ttgttgatag tggcggtaaa ggtttgttat gcgtttacga
    12601 aggattctta aaagcgctta aaggtgaaaa agttgaagcc aaagttgcaa agatagataa
    12661 agatgaattt gtacatgatg aacatgattt ccatggtgta attaatactg aagatattat
    12721 ttatggctat tgtactgaaa tgatggttcg ttttggaaag aataaaaaag cctttgatga
    12781 acaagaattc aggcaagata tgagtcaatt tggtgattct ttattagtca ttaatgatga
    12841 agaaattgtg aaagttcacg tgcataccga atacccaggt aaagtgttta attatggtca
    12901 acaatatggt gaattaatta aacttaaggt tgaaaatatg agagaacagc accgtgaagt
    12961 gattcgaaaa gaacagcaca cagctaaacc gaaaatggaa acggttgaaa cagcaattat
    13021 tactatttct atgggtgaag gtatttcaga gatattcaaa tcaatgggtg ccacacatat
    13081 cattagtggt ggacaaacga tgaatccttc tacagaagat atcgttaaag tcattgaaca
    13141 atcaaaatgt aaacgtgcaa ttattttacc gaataataaa aatattttaa tggcaagtga
    13201 acaagcagcg agtattgttg atgcagaagc agttgttatt ccaacgaaat ctattcctca
    13261 aggtataagc gcactattcc aatatgatgt ggacgcaaca cttgaagaaa ataaagcgca
    13321 aatggctgat tcagtaaata acgttaaatc tggttcatta acgtacgctg ttcgtgatac
    13381 gaaaattgat ggcgttgaga ttaaaaaaga cgcgtttatg ggcttgattg aagataagat
    13441 tgtaagcagc caaagtgatc aattaacaac ggttactgag ttgttaaatg agatgttagc
    13501 agaagatagt gaaatattga ctgtgattat tggtcaagat gcagagcaag cagttacaga
    13561 taacatgata aactggatcg aagagcaata tccagatgta gaagtggaag ttcatgaagg
    13621 tggacaacca atttatcaat atttcttttc agtagaataa aaatttaaaa taaaaaacta
    13681 ccaatgataa atcatcagtt ggtagttttt tattttgcta ttttagtgtt attgcgggtt
    13741 aaaagtatca ttctcgagtt gctaacaatg tcatgttcaa cttagtcatg ataaaataaa
    13801 taacatacta aatgatacgt aaaatcaaat aaaacatagg tgatttattt tggctaaagt
    13861 aaacttaata gaaagtccat attctctttt acaattaaaa ggtataggtc ctaagaaaat
    13921 agaagtattg caacaactaa atattcatac agtggaagat cttgttcttt atttgccaac
    13981 tagatatgaa gataatacag tgattgattt gaatcaagca gaagatcaat ctaacgttac
    14041 gatagaagga caagtatata cagctccagt agttgcattt tttggaagaa ataaatcaaa
    14101 attaaccgtt catttaatgg taaataatat tgctgtcaaa tgtatttttt tcaatcaacc
    14161 gtatttaaaa aagaaaatcg aattaaatca aactataact gttaaaggta agtggaatag
    14221 agttaaacag gaaattactg gtaatagggt tttctttaat tcacaaggga cacaaactca
    14281 agaaaacgca gatgttcaat tagaaccagt ctatcgtatt aaggaaggta ttaaacaaaa
    14341 gcaaatacga gaccaaatta gacaagcgtt aaatgatgtg acaattcatg aatggttaac
    14401 tgatgaacta agagaaaaat ataaattaga gaccttggac tttactttga acacattaca
    14461 tcatcctaaa agtaaagagg atttattacg tgctcgtaga acctatgcat ttactgaact
    14521 gtttttattc gaattacgta tgcaatggct aaatagatta gaaaagtcat ctgacgaagc
    14581 aattgaaatt gattatgact tagaccaagt taaatcattt attgatcgtt taccttttga
    14641 actaactgaa gcacagaaat ccagtgttaa tgaaattttt agagatttaa aagcaccaat
    14701 acgtatgcat cgattacttc aaggtgatgt aggttcagga aaaacagtag ttgctgcaat
    14761 ttgtatgtat gcgttaaaaa cggctggtta tcaatcagca ttgatggtac caactgaaat
    14821 tttagcagag caacatgctg aaagtttaat ggctttattt ggagattcta tgaacgttgc
    14881 attgttaact gggtcagtaa aaggtaagaa acgaaagata cttttagaac aacttgaaaa
    14941 tggtacgatt gattgtttaa ttggaaccca tgctttgatt caagatgatg tgattttcca
    15001 taatgttggt ttagtaatta cagatgaaca acatcgattt ggtgtgaatc aacgccagct
    15061 tttaagagaa aaaggtgcaa tgacgaatgt gttatttatg acagcaacgc cgataccaag
    15121 aacactagca atatcagttt ttggtgagat ggatgtgtct tcaattaaac aattaccaaa
    15181 aggtcgtaaa cctatcatta ctacttgggc aaagcatgag caatacgata aagttttgat
    15241 gcaaatgacc tcagagttga aaaaaggtcg tcaagcatat gtcatttgcc cgctaataga
    15301 aagttctgag catctcgaag atgttcaaaa tgttgtcgca ttgtacgagt ctttacaaca
    15361 gtattatggt gtttcccgtg tagggttatt gcatggtaag ttatctgccg atgaaaaaga
    15421 tgaggtcatg caaaagttta gtaatcatga gatagatgtt ttagtttcta ctactgttgt
    15481 tgaagtaggt gttaatgtac cgaatgcaac ttttatgatg atttatgatg cggatcgctt
    15541 tggattatca actttacatc agttacgcgg tcgtgtaggt agaagtgacc agcaaagtta
    15601 ctgtgtttta attgcatccc ctaaaacaga aacaggaatt gaaagaatga caattatgac
    15661 acaaacaacg gatggatttg aattgagtga acgagactta gaaatgcgtg gtcctggaga
    15721 tttctttggt gttaaacaaa gtgggttgcc agatttctta gttgccaatt tagttgaaga
    15781 ttatcgtatg ttagaagttg ctcgtgatga agcagctgaa cttattcaat ctggcgtatt
    15841 ctttgaaaat acgtatcaac atttacgtca ttttgttgaa gaaaatttat tacatcgcag
    15901 ttttgactaa ttaccatgct gatttgtcaa tttgagtgca acacttcgtt aattgagtga
    15961 tatgacactt gaactattta aatgtaaagt ggtattttaa caatttataa atttccgact
    16021 aaataatagc taaataatac agttatttgt tgagtcggtt aaatagaaag tgttatgata
    16081 tgtgaggaat gtttaagact aggtactaaa aaatgagggg tgagacgttg aaactaaaga
    16141 aagataaacg tagagaagca atcagacaac aaattgatag caatcccttc atcacagacc
    16201 atgaactaag cgacttattt caagtgagta tacaaacaat tcgtttagat cgcacttatt
    16261 taaacatacc agaattaagg aagcgtatta aattagttgc tgaaaagaat tatgaccaaa
    16321 taagttctat tgaagaacaa gaatttattg gtgatttgat tcaagtcaat ccaaatgtta
    16381 aagcgcaatc aattttagat attacatcgg attctgtttt tcataaaact ggaattgcgc
    16441 gtggtcatgt gctgtttgct caggcaaatt cgttatgtgt tgcgctaatt aagcaaccaa
    16501 cagttttaac tcatgagagt agcattcaat ttattgaaaa agtaaaatta aatgatacgg
    16561 taagagcaga agcacgagtt gtaaatcaaa ctgcaaaaca ttattacgtc gaagtaaagt
    16621 catatgttaa acatacatta gttttcaaag gaaattttaa aatgttttat gataagcgag
    16681 gataaaatta tggttaaatt agcaattgat atgatgggtg gcgacaatgc gcctgatatc
    16741 gtattagaag ccgtacaaaa ggctgttgaa gactttaaaa atctagaaat tatgcttttc
    16801 ggtgacgaaa aaaagtataa tctgaaccat gaacgaatcg aatttagaca ttgttctgaa
    16861 aagattgaaa tggaagatga gcctgttaga gcgattaaac gtaaaaaaga tagctcaatg
    16921 gtaaaaatgg ctgaagctgt gaaatctggt gaagcagatg gatgtgtgtc agcaggtaat
    16981 actggtgctt taatgtcagt tggtttattc attgttggac gtattaaagg tgtagctaga
    17041 ccggctttag tagtaacatt gccaacgatt gatggaaaag gttttgtctt tttagacgtt
    17101 ggtgcaaatg ctgatgctaa acctgaacac ttattacagt atgcgcaact aggggatatt
    17161 tatgctcaaa aaattagagg tattgataat ccgaaaatct cattattaaa tataggtacc
    17221 gagccagcta aaggtaatag tttaacgaaa aaatcatttg agttattaaa tcaagatcat
    17281 tcattgaatt ttgttgggaa tattgaagcg aagacattaa tggatggcga tacagatgtt
    17341 gtagttaccg atggctatac tgggaacatg gtccttaaaa atttagaagg tactgcaaaa
    17401 tcaatcggta aaatgttaaa agatacgatt atgagtagta ctaaaaataa attagcaggt
    17461 gcaatattga agaaagattt agcagaattc gctaaaaaga tggattactc agaatacggt
    17521 ggttcagtat tattaggatt ggaaggtact gtagttaaag cacacggtag ttcaaatgct
    17581 aaagcttttt attctgcaat tagacaagcg aaaatcgcag gagaacaaaa tattgtacaa
    17641 acaatgaaag agactgtagg tgaatcaaat gagtaaaaca gcaattattt ttccgggaca
    17701 aggtgcccaa aaagttggta tggcacaaga tttgtttaat aacaatgatc aagcaactga
    17761 aattttaact tcagcagcaa agacgttaga ctttgatatt ttagagacaa tgtttactga
    17821 tgaagaaggt aaattgggtg aaactgaaaa cacgcaacca gctttattga cgcatagttc
    17881 ggcattatta gcagcgctaa aaaatttgaa tcctgatttt actatggggc atagtttagg
    17941 tgaatattca agtttagttg cagctgacgt attatcattt gaagatgcag ttaaaattgt
    18001 tagaaaacgt ggtcaattaa tggcgcaagc atttcctact ggtgtaggaa gcatggctgc
    18061 agtattggga ttagattttg ataaagtcga tgaaatttgt aagtcattat catctgatga
    18121 caaaataatt gaaccagcaa acattaattg cccaggtcaa attgttgttt caggtcacaa
    18181 agctttaatt gatgagctag tagaaaaagg taaatcatta ggtgcaaaac gtgtcatgcc
    18241 tttagcagta tctggaccat tccattcatc gctaatgaaa gtgattgaag aagatttttc
    18301 aagttacatt aatcaatttg aatggcgtga tgctaagttt cctgtagttc aaaatgtaaa
    18361 tgcgcaaggt gaaactgaca aagaagtaat taaatctaat atggtcaagc aattatattc
    18421 accagtacaa ttcattaact caacagaatg gctaatagac caaggtgttg atcattttat
    18481 tgaaattggt cctggaaaag ttttatctgg cttaattaaa aaaataaata gagatgttaa
    18541 gttaacatca attcaaactt tagaagatgt gaaaggatgg aatgaaaatg actaagagtg
    18601 ctttagtaac aggtgcatca agaggaattg gacgtagtat tgcgttacaa ttagcagaag
    18661 aaggatataa tgtagcagta aactatgcag gcagcaaaga gaaagctgaa gcagtagtcg
    18721 aagaaatcaa agctaaaggt gttgacagtt ttgcgattca agcaaatgtt gccgatgctg
    18781 atgaagttaa agcaatgatt aaagaagtag ttagccaatt tggttcttta gatgttttag
    18841 taaataatgc aggtattact cgcgataatt tattaatgcg tatgaaagaa caagagtggg
    18901 atgatgttat tgacacaaac ttaaaaggtg tatttaactg tatccaaaaa gcaacaccac
    18961 aaatgttaag acaacgtagt ggtgctatca tcaatttatc aagtgttgtt ggagcagtag
    19021 gtaatccggg acaagcaaac tatgttgcaa caaaagcagg tgttattggt ttaactaaat
    19081 ctgcggcgcg tgaattagca tctcgtggta tcactgtaaa tgcagttgca cctggtttta
    19141 ttgtttctga tatgacagat gctttaagtg atgagcttaa agaacaaatg ttgactcaaa
    19201 ttccgttagc acgttttggt caagacacag atattgctaa tacagtagcg ttcttagcat
    19261 cagacaaagc aaaatatatt acaggtcaaa caatccatgt aaatggtgga atgtacatgt
    19321 aatatatttg agctaaagct cattgacgca gtggttgact ggtcatccaa tggagaattg
    19381 tctgacctag tcaactttgc gggggaaatt ctaagcaacc tagataaggt tccagaattt
    19441 ctccctaaga aacactaatc aataaattga taagaagatt agaggaacgc ttgagctaaa
    19501 gctcaagata aagacgcaga aatcaataaa ttgataactg ggatagaggt acgcctgagc
    19561 taaagctcaa gataaagacg cagtaatcaa taaattgata actgggatag aggtacacct
    19621 gagctaaagc tcatgcataa gaaacactaa tcaataaatt gataagtgtt tctaaaattt
    19681 ctacttgttt tttagaattt aaaatgggaa aatataatag tctatgtata ggcattttta
    19741 aaggaggtga atcgacgtgg aaaatttcga taaagtaaaa gatatcatcg ttgaccgttt
    19801 aggtgtagac gctgataaag taactgaaga tgcatctttc aaagatgatt taggcgctga
    19861 ctcacttgat atcgctgaat tagtaatgga attagaagac gagtttggta ctgaaattcc
    19921 tgatgaagaa gctgaaaaaa tcaacactgt tggtgatgct gttaaattta ttaacagtct
    19981 tgaaaaataa taaatcttac atctgggtcg tcagtattgt cgactcagtt tttttcttta
    20041 attatcaata gttttaacgt aaaattaaag atgattcaag agcaacacat aaaggagata
    20101 aaataatgtc taaacaaaag aaaagtgaga tagttaatcg ttttagaaag cgctttgata
    20161 ctaaaatgac agagttaggc tttacttatc aaaatattga tttataccaa caagcatttt
    20221 cgcattcgag ttttattaat gattttaata tgaatcgttt agaccataat gagcgtttag
    20281 agtttttggg tgatgcggta ttagaattga cggtttcacg atatttattt gataaacatc
    20341 ccaacttgcc agaagggaat ttaacaaaaa tgcgtgccac tattgtatgt gagccctcac
    20401 ttgtaatatt tgcgaataaa attggattga acgaaatgat tttacttggt aaaggtgaag
    20461 agaaaacagg gggacgtaca agaccatcat taatatcaga tgcattcgaa gcatttattg
    20521 gggcattgta tttggatcaa ggactagata tagtttggaa atttgctgag aaagttattt
    20581 tcccacatgt agaacaaaat gagttattag gcgtggtaga ttttaaaaca caattccaag
    20641 aatatgtgca ccagcaaaat aaaggtgatg taacctataa tttaataaaa gaagagggac
    20701 cggcacatca tcgtctattc acttcagaag ttattctgca aggggaagca atagctgaag
    20761 gtaaagggaa aacgaaaaaa gaatcagaac aacgtgctgc tgaaagtgcc tataagcaat
    20821 taaaacaaat taaatagaaa tttatatgat acgacatata ggtgttcgat ttaggattta
    20881 aggaagaatt tttaatattt tatggaacaa cttcgaaatt taatatacaa atattaaaat
    20941 atcgtataca tgtgttcttt aaaattgtga taaggagttt aggatggttt atttaaaatc
    21001 aatagatgcc attggattta agtcttttgc agatcaaacc aatgttcaat tcgataaagg
    21061 tgtaactgca attgttggtc caaatggaag cggtaaaagt aatattacag atgctattaa
    21121 atgggtgttg ggtgaacaat cggctaaatc attacgtggc tcaaaaatgg aagatattat
    21181 cttctcaggt gcagaacatc gcaaagctca aaattatgct gaagtacagt taagattaga
    21241 taatcattct aaaaagctca gtgttgatga aaacgaagtt attgtaacaa gaagattgta
    21301 tcgaagtggt gaaagtgagt actacataaa taatgaccgt gcaagattaa aagatattgc
    21361 cgatttattt ttagattctg gattgggaaa agaagcgtat agcattatct cgcaaggtag
    21421 agttgatgaa atactaaatg ctaaaccaat tgatagacgt caaattattg aagaatcggc
    21481 tggtgtactt aaatataaaa aacgtaaggc tgaatcatta aataaacttg accaaacaga
    21541 agataattta acgagagtag aagacatttt atatgatttg gaaggtcgcg tagaacctct
    21601 aaaagaggag gcagctatag ctaaagaata taagacactt tcacatcaaa tgaaacatag
    21661 tgacattgta gttacagtgc acgatattga tcaatataca aatgacaata gacaattaga
    21721 tcaacgttta aatgatttac aaggtcaaca agcaaataaa gaagctgaca agcaacgttt
    21781 aagccaacaa attcaacaat ataaaggtaa acgacatcaa cttgataacg atgttgaatc
    21841 gcttaattat caattagtaa aagctacgga agcctttgaa aaatatacgg gacaattaaa
    21901 tgttttagaa gaacgtaaga aaaatcaatc tgaaacaaat gcacgatatg aagaagaaca
    21961 agaaaattta atggagcttt tagaaaatat atcaaatgag atttctgaag ctcaagatac
    22021 ttataagtct ctgaaaagta aacaaaaaga actcaatgct gtcattcgtg aacttgaaga
    22081 acaactatat gtttcagacg aagcacatga tgaaaaattg gaagaaatta aaaacgaata
    22141 ctatacatta atgtcagagc aatcagatgt taacaatgat attcgttttt taaagcatac
    22201 tatagaagag aatgaggcta aaaaatcaag actagattct cgattagttg aagtttttga
    22261 gcaattgaaa gatattcagg gtcaaataaa aacgacaaaa aaagaatatc aacagaccaa
    22321 caaagaactt tctgctgtag ataaagaaat taaaaatata gaaaaagatc tcactgatac
    22381 aaaaaaagca caaaatgaat acgaagagaa attgtatcaa gcatatcgat ataccgaaaa
    22441 aatgaaaaca cgtattgata gtttggcaac gcaagaggaa gaatacactt attttttcaa
    22501 tggcgtcaaa catattttga aagctaaaaa taaagaatta aagggtattc atggtgcagt
    22561 tgcggaaatt attgatgtgc catctaaatt aactcaggca attgaaacag cattaggtgc
    22621 ttcattacaa catgtcattg tagattcaga aaaagatgga cgccaggcta ttcaattttt
    22681 aaaagaacgt aatttaggtc gtgcgacgtt tttaccatta aatgttatac agagtagagt
    22741 ggtagcgact gatattaaat ctattgctaa agaggcaaac ggatttatta gtattgcttc
    22801 ggaagcagtg aaagtagcac cagaatatca aaatattatc gggaatttat taggtaatac
    22861 gattatcgtt gatcatttaa agcatgcaaa tgaattggca cgtgcgatta aatatcgaac
    22921 tcgtattgtt actttggaag gtgatattgt aaaccctggt ggttctatga ctggtggtgg
    22981 cgctcgtaag tccaaaagta ttctgtctca aaaagacgag ttgacaacaa tgagacacca
    23041 attagaagat tacttgcgtc aaacagaatc atttgaacaa caatttaaag agttgaagat
    23101 aaaaagtgat caattaagtg aactgtattt tgaaaaaagt caaaagcata atacacttaa
    23161 agagcaagtg catcattttg aaatggagct cgatagatta actacacaag aaacacaaat
    23221 aaaaaatgat catgaagaat tcgaatttga aaaaaatgat ggttatacga gtgacaaaag
    23281 tcgacaaact ttgagtgaaa aagaaactca tttagaaagt attaaagcat ctttaaaacg
    23341 actagaagat gaaattgaac gctacacaaa actttctaaa gaaggtaagg aaagcgtaac
    23401 taaaacacaa caaacgttac atcagaaaca atctgatctt gctgtggtta aagagcgtat
    23461 taaaacacaa caacagacaa tagatcgatt aaataatcaa agtcaacaaa ctaaacatca
    23521 attaaaagat gttaaagaaa aaattgcatt ctttaattcg gatgaagtga tgggcgaaca
    23581 agcttttcaa aatattaaag atcaaattaa tggtcaacaa gaaacgagaa cacgcttatc
    23641 tgatgaatta gataaattga aacaacaacg tattgagttg aatgaacaaa tcgatgcgca
    23701 ggaagctaca ctacaagttt gtcaccaaga tattttagct atcgaaaatc actaccaaga
    23761 tattaaagct gaacaatcaa agttagatgt attaattcat catgcgatag atcatttaaa
    23821 tgatgaatat caattgactg ttgaacgtgc gaaatctgaa tatacgagtg atgaatcgat
    23881 tgacgcatta cgtaaaaaag ttaagttaat gaagatgtcg attgatgaac taggtcctgt
    23941 aaacttaaat gcaattgaac aatttgaaga gttaaatgaa cgttatacat ttttaagtga
    24001 acaacgtaca gatcttcgta aagctaaaga aacattagag caaattataa gtgaaatgga
    24061 tcaagaggtt actgaaagat ttaaagaaac tttccatgct attcaaggac attttacagc
    24121 tgtgttcaaa caattgtttg gtggaggcga tgcagaattg caattaactg aagccgatta
    24181 tttaacagct ggtattgata ttgtggtaca accaccgggt aaaaagttgc aacatttatc
    24241 gttactgagt ggtggtgagc gtgcattaac tgctattgct ttactatttg caattttaaa
    24301 agtaagatct gcaccttttg ttatattaga tgaggttgaa gctgcactag atgaagcaaa
    24361 tgttattaga tacgcaaaat atttaaatga gttatcagac gaaacacaat tcattgttat
    24421 tacacaccgt aaaggaacaa tggaatttgc agataggtta tacggtgtaa caatgcaaga
    24481 atcaggtgtt actaaacttg tgagtgtgaa tttaaataca atagatgatg tgttgaagga
    24541 ggagcaataa tgagcttttt taaacgctta aaagataagt ttgcaacaaa taaagaaaat
    24601 gaagaagtta aatccttaag agaagaacaa ggtcaagaca aattagaaga tacacattct
    24661 gaaggttcaa cgcaggacgt aaatgattta gcagaaaatg ctgaagtgaa aaagaagcca
    24721 cgcaagttga gtgaagcgga ttttgatgac gatggcttaa tatcaattga agattttgaa
    24781 gaaattgaag ctcaaaaaat gggtgctaaa tttaaagcag gactcgaaaa atctcgtcaa
    24841 aatttccaag aacaattaaa taatttgata gcgagatatc gtaaagtaga tgaagacttt
    24901 tttgaagctt tagaagaaat gttaatcact gcagacgtcg gttttaatac agtgatgacg
    24961 ttaactgaag aattacgtat ggaagcacaa cgacgtaata ttcaagatac tgaagatttg
    25021 cgtgaagtca ttgttgaaaa aatcgtagag atttaccatc aagaagatga taattcagaa
    25081 gctatgaaca tagaagatgg tcgtttaaat gtcattttaa tggtcggtgt gaatggtgtt
    25141 ggtaaaacaa caacaattgg aaaattagct taccgatata aaatggaagg taaaaaagta
    25201 atgttagctg cgggcgatac ttttagagcg ggtgctattg atcaattgaa agtttggggc
    25261 gaacgtgttg gtgtagatgt aattagccaa agtgaaggtt ctgatccagc tgctgttatg
    25321 tatgatgcga ttaatgccgc taaaaacaaa ggtgttgata ttttaatctg tgataccgct
    25381 ggacgtttac aaaataaaac aaatctaatg caagaattag aaaaagttaa gcgtgtaatt
    25441 aatcgagcag tgccagatgc gcctcatgaa gcattactat gtttagatgc tacaactggt
    25501 cagaatgcgt tgtcacaagc tagaaacttt aaagaagtaa caaatgttac aggtattgta
    25561 ttaacgaaat tagatggtac agccaaaggt ggtatcgtat tagccattcg taatgaattg
    25621 cacattccag ttaaatatgt aggtttaggt gagcaattag atgacttaca accatttaac
    25681 cctgaaagtt atgtctacgg cttattcgct gatatgattg aacaaaatga cgaaataaca
    25741 acagttgaaa atgatcaaat tgtaacagaa gaaaaggacg ataatcatgg ggcaaaatga
    25801 tttagttaaa acgttacgaa tgaattattt gtttgatttt tatcaatcct tattgacgaa
    25861 taaacaacgt aattatttgg aattatttta tctcgaagat tattctttaa gtgaaatcgc
    25921 agatactttt aatgtgagta gacaagcagt ttatgataat ataagaagaa ctggcgattt
    25981 agttgaagat tatgaaaaga aattggaatt ataccagaaa tttgagcaac gccgagaaat
    26041 atatgatgaa atgaaacaac atttaagtaa tccagaacaa atacaacgtt atattcaaca
    26101 attagaagac ttagaataga tataaattga gggaggacat cgatatggca tttgaaggct
    26161 tatcagaacg cctgcaagcg acgatgcaaa aaatgcgtgg taagggtaaa cttactgaag
    26221 ctgatataaa gataatgatg cgtgaagtaa gattagcgtt atttgaggct gacgtaaact
    26281 ttaaagtggt aaaagaattt attaaaacag tatcagaacg cgcattaggt tccgatgtaa
    26341 tgcaatcatt aacaccaggg caacaagtta ttaaaatagt tcaagatgaa ttaacgaagt
    26401 tgatgggtgg agaaaataca tcgattaata tgtcaaataa accacctact gttgttatga
    26461 tggttggttt acaaggtgct ggtaaaacaa caactgcagg taaattagca ttattgatgc
    26521 gtaaaaaata caacaaaaaa cctatgttag ttgcagcaga tatttatcgt ccagcagcga
    26581 taaatcaatt acaaacagta gggaaacaaa ttgatattcc tgtatacagt gaaggagatc
    26641 aagtaaagcc acaacaaatt gtaactaatg cattaaaaca tgctaaagaa gaacatttag
    26701 actttgtaat cattgataca gcaggtcgat tacacatcga tgaagcattg atgaacgaat
    26761 taaaagaagt aaaagaaatt gctaaaccaa acgaaattat gttagttgtc gattcaatga
    26821 cgggtcaaga tgctgtcaat gttgcagaat cttttgacga tcaacttgat gtcacaggtg
    26881 ttaccttaac taaattagat ggtgatacac gtggtggtgc agctttatct attcgttcgg
    26941 tgacacaaaa accaattaaa tttgttggta tgagtgaaaa gttagatggt ttagagctat
    27001 tccatcctga acgtatggca tcacgtattt taggtatggg tgatgtgtta agtttaattg
    27061 aaaaagcgca acaagatgtg gatcaagaaa aagcaaaaga tttagagaaa aagatgcgtg
    27121 agtcatcgtt tactttagat gattttttag aacaacttga tcaggtgaaa aatctaggac
    27181 cactggatga tattatgaaa atgattccag gtatgaataa aatgaaaggg ctagataagc
    27241 ttaatatgag tgaaaagcaa attgatcata ttaaagcgat tatccagtca atgacgccgg
    27301 ctgaaagaaa caatccagac acattgaatg tatcacgtaa aaagcgtatt gctaaagggt
    27361 ctggtcgttc attacaagaa gtcaatcgtt tgatgaaaca atttaacgat atgaagaaaa
    27421 tgatgaaaca attcactggt ggcggtaaag gtaaaaaagg taaacgcaat caaatgcaaa
    27481 atatgttaaa aggtatgaat ttaccgtttt aatgtttaat ataacacgct gatttttgtt
    27541 gcaatgagat gtgcatcccg aaagttatac taaagaatat aactttatgg ggtgcatatt
    27601 tttagataca caataataat taaaaactga aattgcggat ttgctagaac atgaggttat
    27661 tgttgctaat attaataagt tatattggtg gaacatgtgc gttagaagtg gtaccattca
    27721 tgtccttacg agataaatta acttaacacg taggtggtga aggctactat gaatttttat
    27781 aacctattaa cagtctaatt tagtaatcac atagttatta ttgtaagaaa aaggttcaat
    27841 aattttcctg gtaaagaaaa aactctttac aaacattcat acacctgtta atattatttc
    27901 ttgtagaaaa taaaaattaa aacatgactt aaaggagatt ttataaatgg cagttaaaat
    27961 tcgtttaaca cgtttaggtt caaaaagaaa tccattctat cgtatcgtag tagcagatgc
    28021 tcgttctcca cgtgacggac gtatcatcga acaaatcggt acttataacc caacgagcgc
    28081 taatgctcca gaaattaaag ttgacgaagc gttagcttta aaatggttaa atgatggtgc
    28141 gaaaccaact gatacagttc acaatatctt atcaaaagaa ggtattatga aaaaatttga
    28201 cgaacaaaag aaagctaagt aatttagcgt aaaattgttc taacaataag aataactcgt
    28261 ttacactgac agttattact caatgatacg ttgggaatat cacatgttag taatatagaa
    28321 cgtattgggt accataatgg tgcccttttt ctttgaatta ttttcaatta aaatagaagt
    28381 gttcaaagca tagagttgga ggtaatagaa tgagagttga agttggtcaa attgttaaca
    28441 cacatggtat taaaggtgaa attaaagtaa aatccaattc agactttaca gacgttcgtt
    28501 ttcaacccgg tcaagtgctg acagttgtgc ataacaataa cgaccttgaa tatactgtta
    28561 agtcacatag agtgcataaa gggcttcata tgcttacatt tgaaggtatt aataatatta
    28621 atgatattga gcatttaaaa gggagttcta tttatcaaga gcgtgatcat gaagatatcg
    28681 tacttgagga aaatgaattt tattattcag atattatagg atgtacagtt tttgatgatc
    28741 aagaaacacc aataggtcgt gtaattaata tatttgaaac aggtgcgaat gatgtgtggg
    28801 tgattaaagg atctaaagaa tatttgattc cttatattgc tgatgttgta aaagaagtgg
    28861 atgttgaaaa taaaaaaatt atcatcacgc caatggaagg attgttggat taatgaaaat
    28921 tgattattta actttatttc ctgaaatgtt tgatggtgtt ttaaatcatt caattatgaa
    28981 acgtgcccaa gaaaacaata aattacaaat caatacggtt aattttagag attatgcaat
    29041 taacaagcac aaccaagtag atgattatcc gtatggtggc ggacaaggta tggtgttaaa
    29101 gcctgaacct gtttttaatg cgatggaaga cttagatgtc acagaacaaa cacgcgttat
    29161 tttaatgtgt ccacaaggcg agccattttc acatcagaaa gctgttgaat taagcaaggc
    29221 tgaccacatc gttttcatat gcggacatta tgaaggttac gatgaacgta tccgaacaca
    29281 tcttgtcaca gatgaaatat caatgggtga ctatgtgtta actggcggtg agttaccggc
    29341 aatgacaatg actgatgcta ttgttagact gattccaggt gttttaggta atgaacagtc
    29401 acatcaagac gattcatttt cagatgggtt attagagttt ccgcaatata cacgtccgcg
    29461 tgaatttaag ggtctaacag ttccagatgt tttattgtct ggaaatcatg ccaatattga
    29521 tgcatggaga catgagcaaa agttgatccg cacatataat aaaagacctg acttaattga
    29581 aaaatatcca ttaactaatg aagataagca aatattagaa agatataaaa taggattgaa
    29641 aaaaggttag tattgtgcta acatattctg agtgtggaaa cacaaaaatc actatgatcc
    29701 gctgctatat atttgtcgag gcaagaacat aggttgaaga ggagaattta taatgacaaa
    29761 tcacaaatta atcgaagcag taactaaatc acaattgcgt acagacttac caagtttccg
    29821 tcctggtgat actttacgtg tacacgtacg tatcattgag ggtactcgtg agcgtatcca
    29881 agtattcgaa ggcattgtaa ttaaacgtcg tggcggtggc gtttctgaaa cgtttacagt
    29941 tcgtaaaatt tcatcaggtg ttggcgtgga acgtacattc ccattacaca caccaaaaat
    30001 tgaaaaaatc gaagttaaac gtcgtggtaa agtacgtcgt gctaaattat attacttacg
    30061 tagtttacgt ggtaaagctg ctagaatcca agaaattcgt taatcagcat ttaaacaaag
    30121 ctatgtatga gtcaaattcg actcaaacaa taaagccatc taggtcactt ttatggagtg
    30181 atatagatgg ctttttttgc tgtttagtta atgtaaagtt gataatgtaa ttgttttttc
    30241 ttatttattc ataacgtgtt tgctcaaaaa tgcttatata aacaaaaatc tactaagtgc
    30301 cattgaaaag gacttagtag atttattaaa tatatgcgta gcaacattat ttttgtcttg
    30361 cccatctcgt gaaaataata ctacatataa tgccaaatat agtaattgtt ataagtaaat
    30421 aatagtatgg tggggtatag ctcaattgaa tctttgttat atttttaggt gctttaatgc
    30481 cggtcataac accatttact tgtgcaactt taagactttg atcacctgat gtcgctttca
    30541 taccttgatt atatgctgtt ggcaaaacaa tatacccaga tgaattttta tttttagtaa
    30601 tagtataacc ttgctttgtc ttactaactt tgacagcctc taatgaattt gaagcgtctt
    30661 taagcgtggt ataatcttcg ccgtatatcc cttttaaatt tactcgatac ttacctttag
    30721 gcaatgataa tctaattcta tctgaagctt taatgcgtat cgttacgggt gttacaaagc
    30781 gtcgatattt ataagtgagt ttatttcttt cttgtgtata ttcattcact ttaacatcat
    30841 gagctttatc cggcgaaagt aattctaaat ccatttcaaa atacaaatct ttaaattgat
    30901 tagaaactga ttttggcaac tgtacagtta gaccaccatt attttgttta acttgtaata
    30961 aatgttttgt aggagattgc caggctgaac tatttaattt aattgttgaa tctgatagta
    31021 aatttttatt ggctttaaaa tgtgtattaa catctttagt attgttagaa acaatccctt
    31081 gcaacattgc ttgttcttta tctaatggag attttaattc tttattggaa aagaccttat
    31141 ttgtaatatg tgcacttgga taatggatgg tatttttaga atgaatccaa cgaactttat
    31201 tgtctttgtg ttcagagtta attttaaatc tatatggtaa gttgtcatca tgattcactc
    31261 taattcgatc attaacattc caaagtgata gtaaattttg acgattgcca agtaatctat
    31321 aagtgctgtt tttatcaatt ggcatattaa tttggagtgt cttgtcataa tattttaaaa
    31381 tgtctccatt aaaaatacta gaatataatg aaatgccatt ataatgatat ataaatggtg
    31441 aatttaatgc atagtctgac atataatcaa tgcgattaaa tgggcctgtt gcattttgat
    31501 ttatcttttt tataagctgg tttacatagt tactatggta atcatgttgt ttcaacgttg
    31561 ataatgattg ttgataaggt ttgattgcca tatttttgtt gttatctaaa atgacgattt
    31621 gttgaatcat aacgattaat actaatattg caacggttaa ttttttataa cgccataaac
    31681 taaattttaa aataacggca agcaccatta gcaggataat acctactata agtgcaagtg
    31741 ggtgtgtcgg tgatagtaat acataaagta atgcgatgat acttactggt attgttctga
    31801 ttaaataata tttcatattt aatgttgata aatgttgaat aaacaatccg caaagagcgc
    31861 ttgatgatag tgctaagata tacacccaac gcctttctgg aaatgaaaaa ccattaaaag
    31921 cactgtcgaa atactgtgat aatgaaccaa taaataatat ccatgttact attgcgaaaa
    31981 gtctataaaa gtaaaaacga tacagtttga atgacaataa tgcaacgata gtaagaattg
    32041 aaatcgtaat ataaaatcca tcgctaaaga aaaaataatg ataatcaagt ggtgtcaaaa
    32101 acggtatatc aacattggga ttttgctttc tgtcattttc caaaaacgca gaaatgccag
    32161 tgaataaacc aaatacactt gataacacac tcaaaactgt agcagatatg acgcaaataa
    32221 atttttgtgt tctagagaca atgtcatatt tgtaagtgaa aatgagtcga tataaatagt
    32281 agcaacctat aataatagct tgataataac tgaaataaaa attgctaaat agtgttaagg
    32341 ctatcgcaac aatgaaaata ccgattttgc gttgttgaaa atatctttcc aaaccaagaa
    32401 tcgataatgg caataaatat aataaatttc cataaaatga ccaagtaaaa ttaaagtata
    32461 taacgacagt tgacatgccg tataaaatcg tagcgatcat atttgttgag cgtttaaagt
    32521 gtaatatttt aaataagtag aaggtcacga caaatgttat gatagctcgt atcatagcca
    32581 taataagttg gtttgtcggc caaaaatgta ttgttgtcgg attaaatata ccaaccgttt
    32641 ctcctatttt aatgaataga aaatttagcc acattaaagg tgacagcgaa taataatatg
    32701 atagtccttt catataatcg ccacctaatc caaacgatgc atcatataaa ctagaaaaac
    32761 tacttagatg ttcatacaaa tacatttgaa atggcatcat ttgacggaat ccatctccag
    32821 ccccactaaa aacagtacca ttcacaatat aatcatagat atgagtagaa aataaaataa
    32881 gcgttaatat tacactaatg aaagttataa caaagaattg tttgacgttt gaatttagcc
    32941 acttttttaa cacaacatta tcctcaactt tcaaatttaa aattaagttt aactgaaact
    33001 aaagttaatg aggttcttga taggtaaaga cgaagatgac tgtggaacag ataccttatc
    33061 atagttactt aaactttgga tcattttcag tttatcatta aacaaatata ttgaataata
    33121 aaaatgtcat actgataaag atgaatgtca cttaataagt aacttagatt taacaaatga
    33181 tgatttttaa ttgtagaaaa cttgaaataa tcacttttac ctaaatctaa agcattgtta
    33241 agaagtgtga caatgttaaa ataaatatag ttgaattaat gaatttgttc tataattaac
    33301 aggttataga ttttaataat gagaaaagaa ttgacgaaag taaggtgaat tgaatggtta
    33361 ttcaatggta tccaggacat atggcgaaag ccaaaagaga agtaagtgaa caattaaaaa
    33421 aagtagatgt agtgtttgaa ctagtagatg caagaattcc atatagttca agaaacccta
    33481 tgatagatga agttattaac caaaaaccac gtgttgttat attaaataaa aaagatatgt
    33541 ctaatttaaa tgagatgtca aaatgggaac aattttttat tgataaagga tactatcctg
    33601 tatcagtgga tgctaagcac ggtaaaaatt taaagaaagt ggaagctgca gcaattaagg
    33661 cgactgctga aaaatttgaa cgcgaaaaag cgaaaggact taaacctaga gcgataagag
    33721 caatgatcgt tggaattcca aatgttggta aatccacatt aataaataaa ctggcaaagc
    33781 gtagtattgc gcagactggt aataaaccag gtgtgaccaa acaacaacaa tggattaaag
    33841 ttagtaatgc attacaacta ttagacacac cagggatact ttggcctaaa tttgaagatg
    33901 aagaagtcgg taagaagttg agtttaactg gtgcgataaa agatagtatt gtgcacttag
    33961 atgaagttgc catctatgga ttaaactttt taattcaaaa tgatttagcg cgattaaagt
    34021 cacattataa tattgaagtt cctgaagatg cagaaatcat agcgtggttt gatgcgatag
    34081 ggaaaaaacg tggcttaatt cgacgtggta atgaaattga ttacgaagca gtcattgaac
    34141 tgattattta tgatattcga aatgctaaaa taggaaatta ttgttttgat atttttaaag
    34201 atatgactga ggaattagca aatgacgcta acaattaaag aagttacgca gttgattaat
    34261 gcggttaata caatagaaga attagaaaat catgaatgct ttttagatga gcgaaaaggt
    34321 gttcaaaatg ccatagctag gcgcagaaaa gcgttagaaa aagaacaagc tttaaaagaa
    34381 aagtatgttg aaatgactta ctttgaaaat gaaatattaa aagagcatcc taatgctatt
    34441 atttgtggga ttgatgaagt tggaagagga cctttagcag gtccagtcgt tgcatgcgca
    34501 acaattttaa attcaaatca caattatttg ggccttgatg actcgaaaaa agtacctatt
    34561 acgaaacgtc tagaattaaa tgaagcacta aaaaatgaag ttactgcttt tgcatatggt
    34621 atcgcgacag ctgaagaaat agatgaattt aatatttata aagctactca aatcgccatg
    34681 cagcgagcta ttgatggatt atcagtacaa ccaacgcatt tattgataga cgcgatgacg
    34741 cttgataatg cactgcctca agtatcttta atcaagggtg atgcaagaag tgtgtccatt
    34801 gcagctgcaa gtatcatggc gaaggttttt cgtgatgatt atatgacaca gttatctaaa
    34861 gattatcctg aatacggttt tgaaaaaaac gcgggttatg gtaccaaaca acatttacta
    34921 gcaatcgatg atattggcat tatgaaagag catagaaaaa gctttgaacc tataaaatcg
    34981 ttactgtaaa tcgtaaaatt caattaaatg ataaaaaagt agcatttaag tttacaatag
    35041 cgcttacatt ttttataatt gtaagggaac ttaacctaag taacaggagg atggaagatg
    35101 aatatccacg agtatcaagg taaagaaata tttcgttcaa tgggcgttgc agttccagaa
    35161 ggacgagtag catttactgc tgaagaagcg gtggagaaag caaaagaatt aaattctgat
    35221 gtatatgttg taaaagcaca aattcatgct ggaggtagag gtaaagcagg cggagtaaaa
    35281 attgctaaat ctttatctga ggtagaaaca tatgcaaaag aattattagg gaaaactttg
    35341 gtgacacatc aaactggtcc agaaggtaaa gaaattaaac gtttatatat cgaagaaggt
    35401 tgtgctattc aaaaagaata ttacgttgga ttcgttattg atcgtgcgac tgaccaagta
    35461 acattgatgg cgtctgaaga agggggcact gagattgaag aagttgctgc gaagactcct
    35521 gaaaagatct tcaaagaaac tatcgatcca gtaatcggac tttcaccatt ccaagcaaga
    35581 cgaattgcgt ttaatattaa tattcctaaa gaatctgtta acaaagctgc taaattctta
    35641 ttagcacttt ataatgtatt cattgaaaaa gattgttcaa tcgtagaaat caacccatta
    35701 gttacaacag ctgatggtga tgtattggca ttagatgcta aaattaattt tgatgataat
    35761 gcattattca gacataaaga tgttgtagaa ttacgtgatt tagaagaaga agatccgaaa
    35821 gagattgaag cgtctaaaca tgatttatca tacattgcat tagatggtga catcggatgt
    35881 atggttaatg gtgcaggttt agccatggca acaatggata cgattaatca tttcggtgga
    35941 aacccagcca atttcttaga tgcaggcgga agcgctacta gagaaaaagt aactgaagca
    36001 tttaaaatca ttttaggtga tgaaaatgtt aaaggtattt ttgtaaacat tttcggtggc
    36061 attatgaaat gtgatgttat cgcagaaggt atcgttgaag ctgtaaaaga agtagattta
    36121 actttaccac tagttgtacg cttagaaggt acaaatgttg agttaggtaa aaaaatctta
    36181 aaagactcag gattagcaat tgaaccagca gcaacaatgg ctgaaggtgc acaaaaaatt
    36241 gttaaactag tcaaagaagc ataaggaaag gatgggagca ctaagatgag tgtatttata
    36301 gataagaata ctaaagtaat ggtacaaggt attacagggt ctactgccct tttccataca
    36361 aaacaaatgc ttgattatgg tacgaaaata gtagcaggtg tgacgcctgg taaaggtggt
    36421 caagttgttg aaggcgttcc tgttttcaac actgttgaag aagctaaaaa tgaaactggg
    36481 gcaacggttt cagtcattta cgttccagca ccatttgctg cagactcaat tttagaagca
    36541 gctgatgcag acttagatat ggttatttgt atcactgaac atattcctgt attagacatg
    36601 gttaaagtta aacgctactt acaaggtaga aaaacacgtt tagttggtcc gaactgtcca
    36661 ggtgtgatta cagcagatga atgtaaaatt ggtattatgc ctggctatat tcacaaaaaa
    36721 ggtcatgttg gtgtagtatc acgttcaggt acactaacat atgaagcagt gcaccaattg
    36781 actgaagaag gtattggtca aactacagct gttggtattg gtggagaccc agtcaacgga
    36841 acaaacttta ttgatgtttt aaaagcattc aatgaagatg acgaaacgaa agcagttgtt
    36901 atgattggtg aaatcggtgg tacagctgaa gaagaagcag ctgaatggat taaagcgaat
    36961 atgacaaaac cagttgtagg ctttatcggt ggacaaatag cacctcctgg aaaacgtatg
    37021 ggacatgctg gtgcaatcat ttcaggtggt aaaggtactg ctgaagagaa aataaaaaca
    37081 ttaaatagtt gtggtgtgaa aacagcggca acaccttcag aaattggttc aacattaatt
    37141 gaagctgcta aagaagcagg tatttatgaa tcattattaa cagttaataa ataaagttaa
    37201 aagatgatat aaatggctat agcccattcc atactttata aaagtatcgg aaatgggcta
    37261 tttgtcgtgc aatgaatttt agtacacttt tcatat
//
LOCUS       DANBWH010000004        34083 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.004, whole genome shotgun sequence.
ACCESSION   DANBWH010000004 DANBWH010000000
VERSION     DANBWH010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 34083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34083
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.004"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            41..391
                     /gene="sarV"
                     /locus_tag="PPY92_000123"
     CDS             41..391
                     /gene="sarV"
                     /locus_tag="PPY92_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarV"
                     /protein_id="HDJ2624155.1"
                     /translation="MSNKVQRFIEAERELSQLKHWLKTTHKISIEEFVVLFKVYEAEK
                     ISGKELRDTLHFEMLWDTSKIDVIIRKIYKKELISKLRSETDERQVFYFYSTSQKKLL
                     DKITKEIEVLSVTN"
     gene            complement(493..1704)
                     /locus_tag="PPY92_000124"
     CDS             complement(493..1704)
                     /locus_tag="PPY92_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDJ2624156.1"
                     /translation="MTNQGSLNKSPIFTKSFTINFFVNFIVYLCMYLLLVVIAGYSKQ
                     AFDASDSLAGLVVGLFIVGSLIGRFATGKFVNQIGPKRLLFIGLIALIITQLLYFIDG
                     SLAFLIFVRLINGIATAVVTTATGTIAAYVTPVNRKSEGISLFSLSLVLGTAIGPFLG
                     MLLITKYAIDLLFIICVILGILGLIISLFIKVDFEVTNNKTETDVINKPRFSIHQFIA
                     KEAIPVAVIMLLIGVTYASILTYLQAFALERHLVTAASYFFICYAIASLITRPIAGRL
                     MDDKNENIIVYPAFIMLFLSFVCLISSYQSWLILLAGACLGLGYGNLSSAMQSIAIKV
                     SPPIKYGIATSTFYVGLDAGVGFGPSFLGLFTHMFSYSEIFDFMAALAIITMLVYFLI
                     HGRHVTRNAMN"
     gene            complement(1697..2137)
                     /locus_tag="PPY92_000125"
     CDS             complement(1697..2137)
                     /locus_tag="PPY92_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDJ2624157.1"
                     /translation="MLSQEFFNSFITIYRPYLKLAEPILEKHNIYYGQWLILRDIAKH
                     QPTTLIEISHRRAIEKPTARKTLKALIENNLITVENSLEDKRQKFLTLTPKGHELYEI
                     VCLDVQKLQQAVVAKTNISQDQMQETINVMNQIHEILLKEAHND"
     gene            2299..3063
                     /locus_tag="PPY92_000126"
     CDS             2299..3063
                     /locus_tag="PPY92_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDJ2624158.1"
                     /translation="MILKFDHIIHYIDQLDRFSFPGDVIKLHSGGYHHKYGTFNKLGY
                     INENYIELLDVENNEKLKKMAKTIEGGVAFATQIVQEKYEQGFKNICLRTNDIEAVKN
                     KLQSEQVEVVGPIQMERDTHKDGKVKWQLLYIMNQDDDEIKPPFFIQWEESDSMRTKK
                     LQKYFQKQFSIETVIVKSKNRSQTVSNWLKWFDMDIVEENDHYTDLILKNDDIYFRIE
                     DGKVSKYHSVIIKDAQATSPYSIFIRGAIYRFEPLV"
     gene            complement(3055..3306)
                     /locus_tag="PPY92_000127"
     CDS             complement(3055..3306)
                     /locus_tag="PPY92_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624159.1"
                     /translation="MRTIILSLFIIMNIVAIIMTLSQPLTVNYFSLRVILIFFTFILS
                     IFFILIKSSRLNNILTILSIVLVIIHMGILAHSTYVYLY"
     gene            3695..4960
                     /gene="femX"
                     /locus_tag="PPY92_000128"
                     /gene_synonym="fmhB"
     CDS             3695..4960
                     /gene="femX"
                     /locus_tag="PPY92_000128"
                     /gene_synonym="fmhB"
                     /EC_number="2.3.2.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase"
                     /protein_id="HDJ2624160.1"
                     /translation="MEKMHITNQEHDAFVKSHPNGDLLQLTKWAETKKLTGWYARRIA
                     VGRDGEVQGVAQLLFKKVPKLPYTLCYISRGFVVDYSNKEALNALLDSAKEIAKAEKA
                     YAIKIDPDVEVDKGTDALQNLKALGFKHKGFKEGLSKDYIQPRMTMITPIDKNDDELL
                     NSFERRNRSKVRLALKRGTTVERSDREGLKTFAELMKITGERDGFLTRDISYFENIYD
                     ALHEDGDAELFLVKLDPKENIAKVNQELNELHAEIAKWQQKMETSEKQAKKAQNMIND
                     AQNKIAKNEDLKRDLEALEKEHPEGIYLSGALLMFAGSKSYYLYGASSNEFRDFLPNH
                     HMQYTMMKYAREHGATTYDFGGTDNDPDKDSEHYGLWAFKKVWGTYLSEKIGEFDYIL
                     NQPLYQLIEQVKPRLTKAKIKISRKLKRK"
     gene            5077..8244
                     /locus_tag="PPY92_000129"
     CDS             5077..8244
                     /locus_tag="PPY92_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDJ2624161.1"
                     /translation="MIKKLLQFSLGNKFAIFLMVVLVVLGGVYASAKLKLELLPNVQN
                     PVISVTTTMPGATPQSTQDEISSKIDNQVRSLAYVKNVKTQSIQNASIVTVEYENNTD
                     MDKAEEQLKKEIDKIKFKDEVGQPELRRNSMDAFPVLAYSFSNKENDLKKVTKVLNEQ
                     LIPKLQTVDGVQNAQLNGQTNREITLKFKQNELEKYGLTADDVENYLKTATRTTPLGL
                     FQFGDKDKSIVVDGQYQSVDAFKNINIPLTLAGGQGQSQSQSDNKQNSAMSDVNQASP
                     QQNSKASAPNNISGMPTAKLGDLADITVGDVRTSISKTNGKDAVNLQITKAQDANTVQ
                     VAKDVQRKIDTFVDENKDFNVTKTMDTAKPVEKSLYTMVEKASLGTIVAIIVILLFLR
                     NIRTTAISIISIPLSLLMALIALKLSDVSLNILTLGALTVAIGRVIDDSIVVVENIYR
                     RLTDSEEQLKGENLIISATTEVFKPIMSSTLVTIIVFLPLVFVSGSVGEMFRPFALAI
                     AFSLLASLLVSITLVPALAATLFKKGVKRRNKQHQEGLGVVSTTYKKVLHWSLNHKWI
                     VIILSTLILVATIVFGGPRLGTSFISAGDDKFLAITYTPKPGETEQAVLNHAKDVEKY
                     LKQKKHVKTIQYSVGGSSPVDPTGSTNSMAIMVEYDNDTPNFDVEADKVIKHVDGFKH
                     PGEWKNQDLGTGAGNKSVEVTVKGPSMDAIKSTVKDIEQKMKQVKGLANVKSDLSQTY
                     DQYEIKVDQNKAAENGISASQLAMHLNENLPEKTVTTVKENGKTVDVKVKQNKQTDWS
                     EDKLNNITLKKPTGGTIKLGDIATLVKTTTPSKLTQEQGDYATTVSAKVTNKDVGGTT
                     RQVMSKINNLDKPNNVKVNIGGASDDINNAMTQLAFAMLAAIIIVYLILVITFKGGLA
                     PFTILFSLPFTVIGVIIALLITGETISVPSLIGMLMLIGIVVTNAIVLIDRVINNEQQ
                     GMEMKEALIEAGGTRIRPILMTAIATIGALVPLLFGQDSSILISKGLAATVIGGLISS
                     TLLTLVVVPVIYEILFTLKKRFTKR"
     gene            complement(8428..8745)
                     /gene="mspA"
                     /locus_tag="PPY92_000130"
     CDS             complement(8428..8745)
                     /gene="mspA"
                     /locus_tag="PPY92_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane stabilizing protein MspA"
                     /protein_id="HDJ2624162.1"
                     /translation="MQFYLILLAILYLIVSFISIFKMEVVFTRILRIIMGVLLLFVLA
                     LTTMSFPKENWWVFIVLLLLVGNVEVTGFKMLKKDLKGVNILNLMSLFIFVIYFILTI
                     VLF"
     gene            complement(8990..9154)
                     /locus_tag="PPY92_000131"
     CDS             complement(8990..9154)
                     /locus_tag="PPY92_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624163.1"
                     /translation="MSLENQLAELKYDYVRLQGDLEKRESLNLDTSALVRQLKDIENE
                     IRNVRAQMQD"
     gene            9287..10195
                     /locus_tag="PPY92_000132"
     CDS             9287..10195
                     /locus_tag="PPY92_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002487080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDJ2624164.1"
                     /translation="MTEQFTMIILLIALGYFLKRINFIKATDSQVIATLVLNVTLPSL
                     VIVNLNSAELKLSFSILPILMIIYGIVAKMIAVWFFRKYDNHMRGSVGMMTGAMNIGL
                     FAYPLIEVIWPKTGLIYFGMADIGGAFVMFGITYFVGSYFSEGSDQFDFKYLGKKLLQ
                     SVPLVTYIVMFILNINHIHLPHVAIDFFSILSHANMPLSMILLGVMLNFTLERKYIPA
                     TIKYLCLHYGLALVAGLLVHVLLPVSDDMIKTTLLVTWMFPVGVAIISYGIQFKYRTL
                     PFIGMTTNLTIIISIIILYVYQAVFV"
     gene            10602..10733
                     /locus_tag="PPY92_000133"
     CDS             10602..10733
                     /locus_tag="PPY92_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624165.1"
                     /translation="MYGAYLINEYHQDFKSIANFKVLKRMINVMMCYCVLFLEPRIS"
     gene            complement(10850..11713)
                     /locus_tag="PPY92_000134"
     CDS             complement(10850..11713)
                     /locus_tag="PPY92_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein GlcU"
                     /protein_id="HDJ2624166.1"
                     /translation="MQFLDFLIALLPALFWGSVVLINVFVGGGPYNQIRGTTLGALIV
                     GLGLLITGFAKFNNPTVIIVGLISGALWAFGQANQLKSISLIGVSNTMPVSTGMQLVG
                     TTLFSVIFLGEWSSMTQIIFGLIAMILLVTGVALTSLKAKNERQSDNPEFKKAMGILI
                     VSTVGYVGFVVLGDIFGVGGTDALFFQSVGMAIGGFILSMNHKTSLKSTALNLLPGVI
                     WGIGNLFMFYSQPKVGVATSFSLSQLLVIVSTLGGIFILGERKDRRQMTGIWAGIIII
                     VIAAIILGNLK"
     gene            complement(11894..12781)
                     /locus_tag="PPY92_000135"
     CDS             complement(11894..12781)
                     /locus_tag="PPY92_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDJ2624167.1"
                     /translation="MSIVQLYDITQIKSFIEHSNYESASYLYKLPQQYNEIDVLITDA
                     IESPGVFSIKENDSIKAIILSFAYDKNKFKVIGPFVADNYVLSVDTFETLFKAMTSNQ
                     PDDAVFNFSFEEGIQQYKPLMKVIQASYNFTDYYIEARTRLEEDMHQPNIIPYHKGFY
                     RAFSKLHTTTFKYQAQSPQDIIDSLDDHHHLFLFVSEGLLKGYLYLEIDSQQSIAEIK
                     YFSSHVDYRLKGIAFELLAYALQYAFDNFDIRKVYFKIRNKNNKLIERFNGLGFHINY
                     EYIKFKFESRNVKDQTIPE"
     gene            12847..12930
                     /locus_tag="PPY92_000136"
     CDS             12847..12930
                     /locus_tag="PPY92_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624168.1"
                     /translation="MILTCYNTFNTIEFKSKISGIDEARSK"
     gene            12956..15091
                     /locus_tag="PPY92_000137"
     CDS             12956..15091
                     /locus_tag="PPY92_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDJ2624169.1"
                     /translation="MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHL
                     VTNATPEQYDKNLKEWRLEDLPIIPKYMKTVVIGKTSKQFKTVKALILDNKVKDIIIA
                     TDAGREGELVARLILDKVGNKKPIRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAAL
                     ARSEADWIVGINATRALTTKYDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYFTLSL
                     TVKGFDFQLESNQRYTNKETLEQMVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQ
                     DMYRRYKIGPKETLNTLQSLYERHKVVTYPRTDSNYLTTDMVDTMKERIQATMATTYK
                     DQARPLMSKTFSSKMSIFNNQKVSDHHAIIPTEVRPVMSDLSNRELKLYDMIVERFLE
                     ALMPPHEYDAITVTLEVAGHTFVLKENVTTVLGFKSIRQGESITEMQQPFSEGDEVKI
                     SKTNIREHETTPPEYFNEGSLLKAMENPQNFIQLKDKKYAQTLKQTGGIGTVATRADI
                     IDKLFNMNAIESRDGKIKVTSKGKQILELAPEELTSPLLTAQWEEKLLLIERGKYQAK
                     TFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTECPTCGKFMIKVKTKNGQMLVCQDPS
                     CKTKKNVQRKTNARCPNCKKKLTLFGKGKEAVYRCVCGHSETQAHMDQRMKSKSSGKV
                     SRKEMKKYMNKNEGLDNNPFKDALKNLNL"
     gene            15204..16538
                     /locus_tag="PPY92_000138"
     CDS             15204..16538
                     /locus_tag="PPY92_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDJ2624170.1"
                     /translation="MKNYFQFDKYGTNFKREILGGITTFLSMAYILAVNPQVLSLAGV
                     KGVSEDMKMDQGAIFVATALAAFVGSLFMGLIAKYPIALAPGMGLNAFFAFTVVLTMG
                     IPWQVGLTGVLFSGIFFAILTVTGFREVIINAIPYQMKMAVSAGIGLFITFVGLQSSG
                     IIVKNESTLVTLGHLTDPPVLLAIFGIVITVILYAIKLPGSIFIGMIITAIVGMFTGL
                     IQMPSGIVGKIPSIEPTFGAAFEAFKDPSQLLTIQFLIVILTFLFIDFFDTAGTLVAV
                     ATQAGIMKDNKLPRAGRALFSDSLATIVGSVFGTTTTTSYIESTSGVAVGARTGFASI
                     VTGCCFLLALFFSPLIAVVTSAVTTPALVVVGVLMAANFAEINWKSFEVAVPAFITII
                     MMPLSYSIATGIACGFIFYPITMLISKKHKEVHPIMYVLMVLFILYFIFVHG"
     gene            complement(16729..17118)
                     /locus_tag="PPY92_000139"
     CDS             complement(16729..17118)
                     /locus_tag="PPY92_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624171.1"
                     /translation="MKNGPKLSLALIGIFLILCEFFYGIPFLGATFILSFGWQPLLFN
                     ALLYLILTIILLVNRQNAIRPIAIIPIFGIVGSFLAIIPFLGILIHWILFFLMILFVL
                     VVLSAPTYIPNKNARVIYTQYKDGHRE"
     gene            17475..17783
                     /gene="rpsJ"
                     /locus_tag="PPY92_000140"
     CDS             17475..17783
                     /gene="rpsJ"
                     /locus_tag="PPY92_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDJ2624172.1"
                     /translation="MAKQKIRIRLKAYDHRVIDQSAEKIVETAKRSGADVSGPIPLPT
                     EKSVYTIIRAVHMYKDSREQFEQRTHKRLIDIVNPTPKTVDALMGLNLPSGVDIEIKL
                     "
     gene            17811..18473
                     /gene="rplC"
                     /locus_tag="PPY92_000141"
     CDS             17811..18473
                     /gene="rplC"
                     /locus_tag="PPY92_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDJ2624173.1"
                     /translation="MTKGILGRKIGMTQVFGENGELIPVTVVEAKENVVLQKKTVEVD
                     GYNAIQVGFEDKKAYKKDAKSNKYANKPAEGHAKKADAAPKRFIREFRNVDVDAYEVG
                     QEVSVDTFVAGDVIDVTGVSKGKGFQGAIKRHGQSRGPMSHGSHFHRAPGSVGMASDA
                     SRVFKGQKMPGRMGGNTVTVQNLEVVQVDTENKVILVKGNVPGPKKGLVEIRTSIKKG
                     NK"
     gene            18500..19123
                     /gene="rplD"
                     /locus_tag="PPY92_000142"
     CDS             18500..19123
                     /gene="rplD"
                     /locus_tag="PPY92_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDJ2624174.1"
                     /translation="MANYDVLKLDGTKSGSIELSDAVFGIEPNNSVLFEAINLQRASL
                     RQGTHAVKNRSAVSGGGRKPWKQKGTGRARQGTIRAPQWRGGGIVFGPTPRSYAYKMP
                     KKMRRLALRSALSFKAQENGLTVVDAFNFEAPKTKEFKNVLSTLEQPKKVLVVTENED
                     VNVELSARNIPGVQVTTAQGLNVLDITNADSLVITEAAAKKVEEVLG"
     gene            19123..19398
                     /gene="rplW"
                     /locus_tag="PPY92_000143"
     CDS             19123..19398
                     /gene="rplW"
                     /locus_tag="PPY92_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002506966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDJ2624175.1"
                     /translation="MEARDILKRPVITEKSSEAMAEDKYTFDVDTRVNKTQVKMAVEE
                     IFNVKVASVNIMNYKPKKKRMGRYQGYTNKRRKAIVTLKEGSIDLFN"
     gene            19431..20264
                     /gene="rplB"
                     /locus_tag="PPY92_000144"
     CDS             19431..20264
                     /gene="rplB"
                     /locus_tag="PPY92_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDJ2624176.1"
                     /translation="MAIKKYKPITNGRRNMTSLDFAEITKTTPEKSLLKPLPKKAGRN
                     NQGKLTVRHHGGGHKRQYRVIDFKRNKDGINAKVDSIQYDPNRSANIALVVYADGEKR
                     YIIAPKGLEVGQIVESGAEADIKVGNALPLQNIPVGTVVHNIELKPGKGGQIARSAGA
                     SAQVLGKEGKYVLIRLRSGEVRMILSTCRATIGQVGNLQHELVNVGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGRAPIGRPSPMSPWGKPTLGKKTRRGKKSSDKLIVRGRKK
                     K"
     gene            20331..20609
                     /gene="rpsS"
                     /locus_tag="PPY92_000145"
     CDS             20331..20609
                     /gene="rpsS"
                     /locus_tag="PPY92_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDJ2624177.1"
                     /translation="MARSIKKGPFVDEHLMKKVEAQEGSEKKQVIKTWSRRSTIFPNF
                     IGHTFAVYDGRKHVPVYVTEDMVGHKLGEFAPTRTFKGHVADDKKTKR"
     gene            20638..20991
                     /gene="rplV"
                     /locus_tag="PPY92_000146"
     CDS             20638..20991
                     /gene="rplV"
                     /locus_tag="PPY92_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDJ2624178.1"
                     /translation="MEAKAVARTIRIAPRKVRLVLDLIRGKNAAEAIAILKLTNKASS
                     PVIEKVLMSALANAEHNYDMNTDELVVKEAYANEGPTLKRFRPRAQGRASAINKRTSH
                     ITIVVSDGKEEAKEA"
     gene            21015..21668
                     /gene="rpsC"
                     /locus_tag="PPY92_000147"
     CDS             21015..21668
                     /gene="rpsC"
                     /locus_tag="PPY92_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDJ2624179.1"
                     /translation="MGQKINPIGLRVGIIRDWEAKWYAEKDFASLLHEDLKIRKFIDN
                     ELKEASVSHVEIERAANRINIAIHTGKPGMVIGKGGSEIEKLRNKLNALTDKKVHINV
                     IEIKKVDLDARLVAENIARQLENRASFRRVQKQAITRAMKLGAKGIKTQVSGRLGGAD
                     IARAEQYSEGTVPLHTLRADIDYAHAEADTTYGKLGVKVWIYRGEVLPTKNTSGGGK"
     gene            21671..22105
                     /gene="rplP"
                     /locus_tag="PPY92_000148"
     CDS             21671..22105
                     /gene="rplP"
                     /locus_tag="PPY92_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDJ2624180.1"
                     /translation="MLLPKRVKYRRQHRPKTTGRSKGGNYVTFGEFGLQATTTSWITS
                     RQIESARIAMTRYMKRGGKVWIKIFPHTPYTKKPLEVRMGAGKGAVEGWIAVVKPGRI
                     LFEVAGVSEEVAREALRLASHKLPVKTKFVKREELGGETNES"
     gene            22095..22304
                     /gene="rpmC"
                     /locus_tag="PPY92_000149"
     CDS             22095..22304
                     /gene="rpmC"
                     /locus_tag="PPY92_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002509269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDJ2624181.1"
                     /translation="MKAKEIRDLTTSEIEEQIKSSKEELFNLRFQLATGQLEETARIR
                     TVRKTIARLKTVAREREIEQSKANQ"
     gene            22328..22591
                     /gene="rpsQ"
                     /locus_tag="PPY92_000150"
     CDS             22328..22591
                     /gene="rpsQ"
                     /locus_tag="PPY92_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDJ2624182.1"
                     /translation="MSERNDRKVYVGKVVSDKMDKTITVLVETYKTHKLYGKRVKYSK
                     KYKTHDENNSAKLGDIVKIQETRPLSATKRFRIVEIVEESVII"
     gene            22623..22991
                     /gene="rplN"
                     /locus_tag="PPY92_000151"
     CDS             22623..22991
                     /gene="rplN"
                     /locus_tag="PPY92_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDJ2624183.1"
                     /translation="MIQQETRLKVADNSGAREVLTIKVLGGSGRKTANIGDVIVCTVK
                     NATPGGVVKKGDVVKAVIVRTKSGVRRNDGSYIKFDENACVIIRDDKGPRGTRIFGPV
                     ARELREGNFMKIVSLAPEVL"
     gene            23027..23344
                     /gene="rplX"
                     /locus_tag="PPY92_000152"
     CDS             23027..23344
                     /gene="rplX"
                     /locus_tag="PPY92_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDJ2624184.1"
                     /translation="MHIKKGDNVKVIAGKDKGKEGKVIATLPKKDRVVVEGVNIMKKH
                     QKPTQLNPEGGILETEAAIHVSNVQLLDPKTNEPTRVGYKFVDGKKVRIAKKSGEEIK
                     SNN"
     gene            23371..23910
                     /gene="rplE"
                     /locus_tag="PPY92_000153"
     CDS             23371..23910
                     /gene="rplE"
                     /locus_tag="PPY92_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDJ2624185.1"
                     /translation="MNRLKEKFNTEVTENLMKKFNYSSVMEVPKIDKIVVNMGVGDAV
                     QNSKVLDNAVEELELITGQKPLVTKAKKSIATFRLREGMPIGAKVTLRGERMYEFLDK
                     LISVSLPRVRDFQGVSKKAFDGRGNYTLGVKEQLIFPEIDYDKVSKVRGMDIVIVTTA
                     NTDEEARELLANFGMPFRK"
     gene            23933..24118
                     /locus_tag="PPY92_000154"
     CDS             23933..24118
                     /locus_tag="PPY92_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDJ2624186.1"
                     /translation="MAKTSMVAKQQKKQKYAVREYTRCERCGRPHSVYRKFKLCRICF
                     RELAYKGQIPGVRKASW"
     gene            24150..24548
                     /gene="rpsH"
                     /locus_tag="PPY92_000155"
     CDS             24150..24548
                     /gene="rpsH"
                     /locus_tag="PPY92_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDJ2624187.1"
                     /translation="MTMTDPIADMLTRVRNANMVRHEKLELPASNIKKEIAEILKSEG
                     FIKNVEYVEDDKQGVLRLFLKYGQNDERVITGLKRISKPGLRVYAKASEMPKVLNGLG
                     IALVSTSEGVITDKEARKRNVGGEIIAYVW"
     gene            24573..25109
                     /gene="rplF"
                     /locus_tag="PPY92_000156"
     CDS             24573..25109
                     /gene="rplF"
                     /locus_tag="PPY92_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDJ2624188.1"
                     /translation="MSRVGKKIIDIPSDVTVTFDGNHVTVKGPKGELSRTLNERMTFK
                     QEENTIEVVRPSDSKEDRTNHGTTRALLNNMVQGVSQGYVKVLELVGVGYRAQMQGKD
                     LILNVGYSHPVEIKAEENITFSVEKNTVVKVEGISKEQVGALASNIRSVRPPEPYKGK
                     GIRYQGEYVRRKEGKTGK"
     gene            25140..25499
                     /gene="rplR"
                     /locus_tag="PPY92_000157"
     CDS             25140..25499
                     /gene="rplR"
                     /locus_tag="PPY92_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDJ2624189.1"
                     /translation="MISKIDKNKVRLKRHARVRTNLSGTAEKPRLNVYRSNKHIYAQI
                     IDDNKGVTLAQASSKDSDIATTATKVELATKVGEAIAKKAADKGIKEIVFDRGGYLYH
                     GRVKALAEAARESGLEF"
     gene            25520..26020
                     /gene="rpsE"
                     /locus_tag="PPY92_000158"
     CDS             25520..26020
                     /gene="rpsE"
                     /locus_tag="PPY92_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDJ2624190.1"
                     /translation="MARREEETKEFEERVVTINRVAKVVKGGRRFRFTALVVVGDKNG
                     RVGFGTGKAQEVPEAIKKAVEAAKKDLVVVPRVEGTTPHTITGRYGSGSVFMKPAAPG
                     TGVIAGGPVRAVLELAGITDILSKSLGSNTPINMVRATIDGLQNLKNAEDVAKLRGKT
                     VEELYN"
     gene            26037..26216
                     /gene="rpmD"
                     /locus_tag="PPY92_000159"
     CDS             26037..26216
                     /gene="rpmD"
                     /locus_tag="PPY92_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDJ2624191.1"
                     /translation="MAKLQITLTRSVIGRPETQRKTVEALGLKKTNSSVVVEDNPAIR
                     GQINKVKHLVTVEEK"
     gene            26233..26673
                     /gene="rplO"
                     /locus_tag="PPY92_000160"
     CDS             26233..26673
                     /gene="rplO"
                     /locus_tag="PPY92_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDJ2624192.1"
                     /translation="MKLHELKPAEGSRKERNRVGRGVATGNGKTSGRGHKGQKARSGG
                     GVRPGFEGGQLPLFRRLPKRGFTNINRKEYAIVNLDQLNKFEDGTEVTPALLVESGVV
                     KNEKSGIKILGNGSLDKKLTVKAHKFSASAAEAIDAKGGAHEVI"
     gene            26673..27965
                     /gene="secY"
                     /locus_tag="PPY92_000161"
     CDS             26673..27965
                     /gene="secY"
                     /locus_tag="PPY92_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDJ2624193.1"
                     /translation="MIQTLVNFFRTKEVRNKIFFTLAMLVIFKIGTYIPAPGVNPAAF
                     DNPQGSQGATELLNTFGGGALKRFSIFAMGIVPYITASIVMQLLQMDIVPKFSEWAKQ
                     GEVGRRKLNNVTRYLAISLAFIQSIGMAFQFNNYLKGALIINQSIMSYLLIALVLTAG
                     TAFLIWLGDQITQFGVGNGISIIIFAGILSTLPASLIQFGQTAFVGQEDTSLAWLKVL
                     GLLVSLILLTVGAIYVLEAVRKIPIQYAKKQTAQRLGSQATYLPLKVNSAGVIPVIFA
                     MAFFLLPRTLTLFYPDKEWAQNIANAANPSSNVGMVVYIVLIILFTYFYAFVQVNPEK
                     MADNLKKQGSYVPGIRPGEQTKKYITKVLYRLTFVGSIFLAVISILPILATKFMGLPQ
                     SIQIGGTSLLIVIGVAIETMKSLEAQVSQKEYKGFGGR"
     gene            27982..28629
                     /locus_tag="PPY92_000162"
     CDS             27982..28629
                     /locus_tag="PPY92_000162"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDJ2624194.1"
                     /translation="MNIILMGLPGAGKGTQASEIVKKFPIPHISTGDMFRKAIKEETE
                     LGKEAKSYMDRGELVPDEVTVGIVKERISEDDAKKGFLLDGFPRTIEQAEALNNIMSE
                     LDRNIDAVINIEVPEEELMNRLTGRRICESCGTTYHLVFNPPKVEGICDIDGGKLYQR
                     EDDNPETVANRLSVNIKQSKPILDFYDQKGVLKNIDGSKDISDVTKDVIDILDHL"
     gene            28822..29040
                     /gene="infA"
                     /locus_tag="PPY92_000163"
     CDS             28822..29040
                     /gene="infA"
                     /locus_tag="PPY92_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDJ2624195.1"
                     /translation="MAKQDVIELEGTVLDTLPNAMFKVELENGHEILAHVSGKIRMNY
                     IRILPGDKVTVEMSPYDLTRGRITYRYK"
     gene            29072..29185
                     /gene="rpmJ"
                     /locus_tag="PPY92_000164"
     CDS             29072..29185
                     /gene="rpmJ"
                     /locus_tag="PPY92_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDJ2624196.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQRQG"
     gene            29208..29573
                     /gene="rpsM"
                     /locus_tag="PPY92_000165"
     CDS             29208..29573
                     /gene="rpsM"
                     /locus_tag="PPY92_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDJ2624197.1"
                     /translation="MARIAGVDIPREKRVVISLTYIYGIGTSTAQKILEEANVSADTR
                     VKDLTDDELGRIREVVDGYKVEGDLRRETNLNIKRLMEISSYRGIRHRRGLPVRGQKT
                     KNNARTRKGPVKTVANKKK"
     gene            29597..29986
                     /gene="rpsK"
                     /locus_tag="PPY92_000166"
     CDS             29597..29986
                     /gene="rpsK"
                     /locus_tag="PPY92_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDJ2624198.1"
                     /translation="MARKQVSRKRRVKKNIENGVAHIRSTFNNTIVTITDEFGNALSW
                     SSAGALGFKGSKKSTPFAAQMASETASKSAMEHGLKTVEVTVKGPGPGRESAIRALQS
                     AGLEVTAIRDVTPVPHNGCRPPKRRRV"
     gene            30061..31005
                     /locus_tag="PPY92_000167"
     CDS             30061..31005
                     /locus_tag="PPY92_000167"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDJ2624199.1"
                     /translation="MIEIEKPRIETIEISEDAKFGKFVVEPLERGYGTTLGNSLRRIL
                     LSSLPGAAVKYIEIEGVLHEFSAVDNVVEDVSTIIMNIKQLALKIYSEEDKTLEIDVR
                     DEGEVTASDITHDSDVEILNPELKIATVSKGGHLKIRLVANKGRGYALAEQNNTSDLP
                     IGVIPVDSLYSPVERVNYTVENTRVGQSSDFDKLTLDVWTNGSITPQESVSLAAKIMT
                     EHLNIFVGLTDEAQNAEIMIEKEEDQKEKVLEMSIEELDLSVRSYNCLKRAGINSVQE
                     LADKSEADMMKVRNLGRKSLEEVKYKLEDLGLGLRKED"
     gene            31022..31390
                     /gene="rplQ"
                     /locus_tag="PPY92_000168"
     CDS             31022..31390
                     /gene="rplQ"
                     /locus_tag="PPY92_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002450507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDJ2624200.1"
                     /translation="MGYRKLGRTSDQRKAMLRDLATSLIISERIETTEARAKEVRSVV
                     EKLITLGKKGDLASRRNAAKTLRNVEILNEDETTQTALQKLFGEIAERYTERQGGYTR
                     ILKQGPRRGDGAESVIIELV"
     gene            31928..32737
                     /locus_tag="PPY92_000169"
     CDS             31928..32737
                     /locus_tag="PPY92_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDJ2624201.1"
                     /translation="MEDKNSVIVFKNVSFQYQSDASFTLKDVSFNIPKGQWTSIVGHN
                     GSGKSTIAKLMIGIEKVKSGEIFYNNQAITDDNFEKLRKDIGIVFQNPDNQFVGSIVK
                     YDVAFGLENHAVPHDEMHRRVSEALKQVDMLERADYEPNALSGGQKQRVAIASVLALN
                     PSVIILDEATSMLDPDARQNLLDLVRKVKSEHNITIISITHDLSEAMEADHVIVMNKG
                     TVYKEGTATEIFDHAEELTRIGLDLPFPIKINQMLGHQTSFLTYEGLVDQL"
     gene            32734..33594
                     /locus_tag="PPY92_000170"
     CDS             32734..33594
                     /locus_tag="PPY92_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDJ2624202.1"
                     /translation="MTIRFDNVSYTYQKGTPYQHQAIHDVNTEFEQGKYYAIVGQTGS
                     GKSTLIQNINALLKPTTGTVTVDDITITHKTKDKYIRPVRKRIGMVFQFPESQLFEDT
                     VEREMIFGPKNFKMNLDEAKNYAHRLLMDLGFSRDVMSQSPFQMSGGQMRKIAIVSIL
                     AMNPDIIVVDEPTAGLDPQSKRQVMRLLKSLQTDENKAIILISHDMNEVARYADEVIV
                     MKEGSIVSQTSPKELFKDKKKLADWHIGLPEIVQLQYDFEQKYQTKLKDIALTEEAFV
                     SLYKEWQHEK"
     gene            33584..34081
                     /gene="efb"
                     /locus_tag="PPY92_000171"
     CDS             33584..34081
                     /gene="efb"
                     /locus_tag="PPY92_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Efb"
                     /protein_id="HDJ2624203.1"
                     /translation="MKNKLIAKSLLTIAAIGITTTTIASTADASEGYGPREKKPVSIN
                     HNIVEYNDGTFKYQSRPKFNSTPKYIKFKHDYNILEFNDGTFEYGARPQFNKPAAKTD
                     ATIKKEQKLIQAQNLVREFEKTHTVSAHRKAQKAVNLVSFEYKVKKMVLQERIDNVLK
                     QGLVR"
BASE COUNT        12404 a         5206 c         6472 g        10001 t
ORIGIN      
        1 attacaaaca tctaagaaga aacataggag gcatcatatt atgagtaata aagttcaacg
       61 ttttatagaa gcagaaaggg agttaagtca gttaaagcac tggttaaaaa caacacataa
      121 gatttcaatt gaagaatttg tagtactttt taaagtgtat gaagctgaaa agattagcgg
      181 taaagaattg agggatacat tacattttga aatgctatgg gatacaagta aaatcgatgt
      241 gattatccgt aaaatctata aaaaagagct tatttctaaa ttgcgttctg aaacggatga
      301 aagacaagta ttctatttct atagtacttc tcaaaagaaa ttgttagata aaattactaa
      361 agaaatagaa gtgttaagcg ttacaaacta aaaacttaaa aagcatgcca atcagtacat
      421 catatttgcg tcttggggac agacaaatga tgaatagaga ttggcatgct ttttattttt
      481 gaatataaat atttagttca tggcatttct agttacatga cgtccatgaa ttaagaagta
      541 aacaagcata gtaatgattg ctaaagcggc cataaagtcg aagatttcac tatatgaaaa
      601 catatgagta aataacccaa ggaatgatgg accgaagccg acacctgcat ctagaccaac
      661 gtaaaaagta gatgtcgcga taccatattt aatcgggggt gagactttta tcgcaataga
      721 ttgcattgca gatgataaat ttccataccc taaacctagg caagcaccag caagtaatat
      781 taaccagctt tgatagcttg aaattaagca cacaaatgaa aggaaaagca tgataaatgc
      841 tggatagaca ataatatttt catttttatc atccatcaat ctaccagcaa taggtctagt
      901 aattaacgat gctatagcat agcaaataaa gaaatagctt gctgcagtga ctaggtgtcg
      961 ctctaaagca aatgcttgta aataagttag gattgacgca taggtaacgc caattaaaag
     1021 cataattaca gcaacaggaa tggcctcttt tgcaataaat tgatgaatac taaatcttgg
     1081 tttattaatg acatcagttt cagttttatt atttgttact tcgaaatcaa cttttataaa
     1141 taatgagata atgagtccga gtatgcctaa tatgacacaa ataataaaca gtaaatcaat
     1201 tgcgtatttt gtaataagta acatgcctag aaatgggcca atcgctgtac ctaatactaa
     1261 acttaaggaa aataaactga tgccttcact ttttctatta acaggggtaa cgtatgccgc
     1321 aatagtacct gttgcagttg tcacaactgc agttgcgata ccgtttatga gacgtacaaa
     1381 gattaaaaaa gctaaagatc catcaataaa ataaagtaat tgcgtgataa ttaaagcaat
     1441 taaaccaata aataataatc gtttaggtcc gatttgattt acaaatttac ctgtagcaaa
     1501 tcgaccaatt aaagacccga caataaatag tcctacaact aatccggcta atgaatcgga
     1561 agcgtcgaaa gcttgcttac tatagccagc aataactaca agtaacaaat acatgcatag
     1621 gtatacgata aagttgacaa aaaagttgat tgtaaaactt ttggtgaaaa taggtgattt
     1681 atttaatgag ccttgattag tcattgtgtg cctcctttaa taatatttca tgaatttgat
     1741 tcatcacatt tatggtttct tgcatttgat cttgcgaaat gtttgttttg gcaactactg
     1801 cttgttggag cttttgtaca tcaagacaaa caatctcata taattcatgc cctttaggtg
     1861 ttaaagttaa aaacttttgt cgtttatcct ctaaactgtt ttctactgtt ataaggttat
     1921 tttctattag agcttttaaa gtttttcttg cagtaggctt ttcaattgca cgtctatgtg
     1981 aaatttcaat gagagtagtg ggctgatgtt tagcgatatc gcgtaagatt aaccattgac
     2041 cataatatat attgtgtttt tccaaaatcg gctctgctaa ttttaaatag gggcgatata
     2101 ttgttataaa actattgaaa aattcttgtg atagcatagt gacatctcct aagacaaaat
     2161 agttagctta gctaaccttt ttacaacaat agtaattata aaacgggagc aattagaaat
     2221 caatatataa ttattaagag caaaaataat tatactttgt taaaataagc gtaattacat
     2281 gtaaataggg ggatactaat gatattgaaa tttgatcaca tcattcatta tatagatcag
     2341 ttagatcggt ttagttttcc aggagatgtt ataaaattac attcaggtgg gtatcatcat
     2401 aaatatggaa cattcaataa attaggttat atcaatgaaa attatattga gctactggat
     2461 gtagaaaata atgaaaagtt gaaaaagatg gcaaaaacga tagaaggtgg agtcgctttt
     2521 gctactcaaa ttgttcaaga gaagtatgag caaggcttta aaaatatttg tttgcgtaca
     2581 aatgatatag aggcagttaa aaataaacta caaagtgagc aggttgaagt agtagggccg
     2641 attcaaatgg aaagagatac acataaagat ggtaaggtaa agtggcaatt gctttatata
     2701 atgaatcagg atgatgatga aattaagcca ccatttttta ttcaatggga agaaagtgat
     2761 tccatgcgta ctaaaaaatt gcaaaaatat tttcaaaaac aattttcaat tgaaactgtt
     2821 attgtgaaaa gtaaaaaccg atcacaaaca gtatcgaatt ggttgaaatg gtttgatatg
     2881 gacattgtag aagagaatga ccattacaca gatttgattt taaaaaatga tgatatttat
     2941 tttagaattg aagatggtaa agtttcaaaa tatcattcgg ttatcataaa agacgcacaa
     3001 gcaacttcac catattcaat ttttatcaga ggtgctattt atcgctttga accattagta
     3061 taaatatacg taagtgctat gagcgagaat gcccatatga ataatgacaa gcacaatgga
     3121 aagaatcgtt aatatattat ttaatcggga tgacttaatt aaaatgaaaa agattgataa
     3181 tataaatgtg aaaaagataa gtataacccg taaactaaag taattcacgg tgagaggttg
     3241 actcaatgtc ataatgattg caacgatgtt cataattata aatagactta aaataattgt
     3301 tctcatatca aacacctcat tgttagatta ttgacattat aacaggggta attgtatatg
     3361 aacattaatg tggttgcttg aggaaaaatt tattcattga agtcaagttg gttcatttta
     3421 gaaatgaata tcgtgttaga tgatgaaagt atattgaagt ataggtaact agttgaaaag
     3481 tattaattgt acgataacat taaatttaac acgaaacata gatataaaat gattcacaat
     3541 taaaatgggt aaatttgaac ttgctaaact attaattgga gcatggacat ttcaaaaata
     3601 agagttcaaa tcttacacaa gctctgaatc gacactataa gatacaaact gtataattaa
     3661 aggtattgtt aaatagaagg agatatcata aatcatggaa aagatgcata tcactaatca
     3721 ggaacatgac gcatttgtta aatctcaccc aaatggagat ttattacaat taacgaaatg
     3781 ggcagaaaca aagaaattaa ctggatggta cgcgcgaaga atcgctgtag gtcgtgacgg
     3841 tgaagttcag ggtgttgcgc aattactttt taaaaaagta cctaaattac cgtatacgct
     3901 atgttatatt tcacgtggtt ttgttgttga ttatagtaat aaagaagcgt taaatgcatt
     3961 gttagacagt gcaaaagaaa ttgctaaagc tgagaaagcg tatgcaatta aaatcgatcc
     4021 tgatgttgaa gttgataaag gtacagatgc tttgcaaaat ttgaaagcgc ttggttttaa
     4081 acataaagga tttaaagaag gtttatcaaa agactacatc caaccacgta tgactatgat
     4141 tacaccaatt gataaaaatg atgatgagtt attaaatagt tttgaacgcc gaaatcgttc
     4201 aaaagtgcgc ttggctttaa agcgaggtac gacagtagaa cgatctgata gagaaggttt
     4261 aaaaacattt gctgaattaa tgaaaatcac tggggaacgc gatggcttct taacgcgtga
     4321 tattagttac tttgaaaata tttatgatgc gttgcatgaa gatggagatg ctgaactatt
     4381 tttagtaaag ttggacccaa aagaaaatat agcgaaagta aatcaagaat tgaatgaact
     4441 tcatgccgaa atagctaaat ggcagcagaa gatggaaaca tctgaaaagc aagctaaaaa
     4501 agcgcaaaat atgattaatg atgcgcaaaa taaaattgct aaaaatgaag atttaaaacg
     4561 agacctagaa gctttagaaa aggaacatcc tgaaggtatt tatctttctg gtgcactatt
     4621 aatgtttgct ggctcaaaat catattactt atatggtgcg tcttctaatg aatttagaga
     4681 ttttttacca aatcaccata tgcagtatac gatgatgaag tatgcacgtg aacatggtgc
     4741 aacaacttac gatttcggtg gtacagataa tgatccagat aaagactctg aacattatgg
     4801 cttatgggca tttaaaaaag tgtggggaac atacttaagt gaaaagattg gtgaatttga
     4861 ttatatattg aatcagccat tgtaccaatt aattgagcaa gttaaaccgc gtttaacaaa
     4921 agctaaaatt aaaatatctc gtaaattaaa acgaaaatag attaacgact gaaatctgaa
     4981 cgctcataag actgtcattt gcgttcagat ttttttacac aatatagaat ggttgagtaa
     5041 aatatttttg aatatagtga aagaggggga agtactgtga taaaaaagct attacaattt
     5101 tctttgggga ataagtttgc tatcttttta atggttgttt tagttgtctt gggcggtgta
     5161 tacgcgagtg ctaaattgaa attagaatta ctaccaaatg tacaaaatcc agttatttca
     5221 gttacaacaa caatgccggg tgcaacgcca caaagtaccc aagatgaaat aagtagtaaa
     5281 attgacaatc aagtaagatc gttggcatat gtgaaaaatg ttaaaacgca atccatacaa
     5341 aatgcttcaa ttgtaacagt tgaatatgaa aataatacag atatggataa agcagaagaa
     5401 cagcttaaaa aagaaatcga taaaattaaa tttaaagatg aagttggtca accagaatta
     5461 agacgtaatt cgatggacgc ttttccggtt ttagcatatt cattttcaaa taaagagaat
     5521 gacttgaaaa aagtaacgaa agtactgaat gaacaattaa taccaaaatt acaaacggta
     5581 gatggtgtgc aaaatgcgca attaaatggg cagacgaacc gtgaaatcac ccttaaattt
     5641 aagcaaaatg aacttgaaaa atatgggttg actgctgatg atgtagaaaa ctatctaaaa
     5701 acagcaacaa gaacaacacc acttggattg ttccaatttg gtgataaaga taaatcaatt
     5761 gttgttgatg gtcaatatca atctgttgat gcttttaaaa acataaatat tccattaacg
     5821 ttggcaggag ggcaagggca atctcaatcc caaagtgata ataaacaaaa ttcagccatg
     5881 tcagacgtta atcaggcatc accgcagcaa aattcaaaag cgtcagcacc aaataatata
     5941 agtggtatgc cgacagcgaa actaggagat ttagctgata ttacagttgg tgatgtgcgt
     6001 acttctattt ctaaaacgaa tggaaaggat gcggttaatc tacaaataac taaagctcaa
     6061 gatgccaata cagttcaagt agccaaagat gtacaacgta aaattgatac attcgttgat
     6121 gaaaataaag attttaatgt cacaaaaaca atggatactg caaagcctgt tgagaaatca
     6181 ctttatacga tggttgaaaa agcatcatta ggtacaatcg tggcaattat agttattttg
     6241 ctgtttttaa gaaacattcg tacgacggca atttctatta tatccattcc gttatcactt
     6301 cttatggcgc ttattgctct gaaattgagt gatgtttcat tgaatatact aacgttaggt
     6361 gcattaacag tagcgattgg acgtgtgata gacgattcga ttgtagttgt tgaaaatatt
     6421 tatcgacgct taacagattc agaagaacaa ctaaaaggtg aaaatttaat tatcagtgcg
     6481 acaactgaag tatttaaacc aataatgtca tcgacactag ttactattat cgtcttctta
     6541 ccacttgtgt ttgtatcagg ttcagtaggc gaaatgttta gaccttttgc attggctatt
     6601 gcatttagtt tattagcatc gttattagtg tcaattacac tcgttccagc tttggcagca
     6661 acactattta aaaaaggcgt taaacgtcgt aataaacaac atcaagaagg attaggtgtt
     6721 gttagtacaa cttataaaaa agtattgcat tggtcactaa atcataagtg gattgtaatt
     6781 atattaagta cattaatttt ggttgcaact attgtatttg gaggaccgag actaggcact
     6841 agctttattt cagcaggtga cgataaattt ttagctatta cttatacacc gaagcctggt
     6901 gaaacggagc aagcagtgtt gaatcatgcg aaagatgttg aaaaatattt aaaacagaaa
     6961 aagcatgtaa aaacaattca atactcagtt ggtggtagta gtccagtaga tccaacgggt
     7021 agtacaaata gtatggcaat catggttgaa tatgataatg acacgcctaa ttttgatgta
     7081 gaagcggata aggttattaa acatgtagat ggctttaaac atcctggaga gtggaaaaat
     7141 caagatttag gaacaggtgc aggtaataaa tctgtagagg ttactgtgaa aggcccatca
     7201 atggatgcca taaaatcaac tgtaaaagat attgaacaga aaatgaaaca ggttaaagga
     7261 ctagccaatg tcaaatctga tttatcgcaa acatatgatc agtatgaaat taaagttgat
     7321 caaaataaag cggcagaaaa tggtatttct gcaagtcaac ttgcaatgca cttgaatgaa
     7381 aacttaccag aaaaaacagt tacgactgtt aaagaaaatg gtaaaactgt tgatgttaaa
     7441 gtcaaacaaa ataagcaaac agactggtca gaagataagt tgaataatat aactttgaaa
     7501 aagccgactg gtggtacgat taaattggga gatatcgcta cgttagttaa aacaacgaca
     7561 ccaagtaaat tgacgcaaga acaaggtgat tatgcaacga cggtatctgc taaagtaaca
     7621 aataaagatg tgggtggcac aacacgacaa gtgatgtcta aaataaataa tttggacaaa
     7681 ccgaataatg taaaggttaa tatcggtggt gcatcagatg atattaacaa tgcaatgaca
     7741 caattagcct ttgcaatgtt agctgcaatc attatcgtat atttaatcct agttattaca
     7801 tttaaaggtg gcttagcgcc atttacaatt ttattctctt taccatttac agttatcggt
     7861 gtaattattg cactattaat cacaggagaa acaatatcag taccaagttt aattggtatg
     7921 ctaatgttaa ttggaatcgt agtaacaaat gccattgtgt taatagaccg tgtcattaat
     7981 aatgagcagc agggcatgga gatgaaagaa gcattaatcg aagcaggcgg tactagaatt
     8041 agaccgatat taatgacggc gattgcaaca atcggtgcat tagttccttt gttatttggt
     8101 caagatagct cgattcttat ttcgaaagga ttagctgcta cagttattgg tggtttaatt
     8161 tcatcaactt tattaacact tgtagttgta ccagtaatat atgaaattct ctttacttta
     8221 aaaaaacgat tcactaaacg atagataata taaaagccat aaaagcggtc atgatattga
     8281 ctacttttat ggctttttaa aatttgaaat tttagacctt atttcacgtg cgatttaatc
     8341 gatgtgttaa taaaacaata ctggcattca acatgtacga taataaaata aaattgcaat
     8401 tatgttattg catgttttgt ttattattta gaataatacg atggttaaga tgaaatatat
     8461 gacaaagata aataatgaca ttaaattcaa gatgtttaca ccttttagat cttttttaag
     8521 cattttaaat cctgtcactt cgacattgcc gactaagagt agtaagacga taaataccca
     8581 ccaattctct tttggaaaac tcatcgtcgt taatgctaag acgaacaata acaacacacc
     8641 cataataatt ctcaaaatgc gagtaaatac aacttccatt ttaaaaatac taataaaact
     8701 aacaattaga taaagtattg ctagtaaaat cagataaaat tgcatatgat aacttcctct
     8761 ccgtttcaaa gggttcatgt agtacagcag catagaaaac ataaatagca gtgttgggat
     8821 agatatgcgt gtatgttgtt aatgaaacac taaatcataa cacgacaaca tcttgtgatt
     8881 ctagatgtaa ttgagcgaaa aataaagcaa ttcatcaata taacgtattc agagcaatgc
     8941 tagtcaaatt tatgtaacga aaagcattta ttgcttgaat gatagattat taatcttgca
     9001 tttgagcgcg aacgtttcta atttcatttt caatatcttt aagttgacga acaagtgcgg
     9061 aagtatctaa attcaaagat tcccgttttt ctaagtcacc ttgaagacga acataatcat
     9121 atttaagttc ggctagttgg ttttctaaag acatactata acactccttt gaaatataat
     9181 taagtgcatt taatgtagat tatagcatga aacagtagac ttctaaaata tctgtggagt
     9241 atgttaaagt tataaattat gaagattatt gaaagaaggg ataacagtga cagaacaatt
     9301 tactatgatt attttattaa ttgcgcttgg ttactttttg aagcgcatta attttataaa
     9361 ggcaacagat agtcaagtca ttgctacatt agttcttaat gtcacactcc catctttagt
     9421 tattgtgaat ttaaacagtg cagaattaaa attgtcattc tccattttac caattttaat
     9481 gattatatat ggtattgtgg cgaaaatgat tgctgtctgg ttttttagaa aatatgataa
     9541 tcacatgcgt ggatcagtag gtatgatgac tggtgctatg aatattggat tgtttgcata
     9601 tccactaatt gaagtgatat ggcctaaaac ggggcttatt tattttggta tggctgatat
     9661 aggtggtgcc tttgtgatgt ttggaattac ttactttgta ggtagctact ttagtgaagg
     9721 tagtgatcaa ttcgatttta aatatttagg taagaaatta ttacagtcag tgccactcgt
     9781 tacatacatt gtaatgttta tattgaatat aaatcacatc catttaccac atgtggcaat
     9841 cgatttcttt agcatacttt cccatgcaaa tatgccgtta tcaatgattt tgctaggtgt
     9901 tatgttgaat tttactctgg aaagaaagta tatacctgct actattaaat atttatgttt
     9961 gcattatgga ttagcattag ttgcgggatt gttagtacat gtgttgttac cggtatcaga
    10021 tgatatgatt aagacgacat tgttggtcac ttggatgttc ccggttggtg tcgcaattat
    10081 ttcttatggt attcagttta aatatcgaac actgccattt ataggtatga cgaccaattt
    10141 gacaatcatt attagtatca ttattttgta cgtgtatcag gcagtgtttg tctagtttac
    10201 acatttgaaa ataaaatcca ttttagtatt cgtagaaaat gagagccccc tttaccacaa
    10261 catgtaaatg catattgtga aaagggggct tttatttatg acatagttgt tcaacaggcc
    10321 gggacataaa tcaatattct atgctctacg aagttatatt ggcagtagtt tactgaacga
    10381 aaatgcgctt gtaacaagct tttttcaatt ctagtcaggg gccccaacac agaagttgac
    10441 gaaaagtcag cttacaataa tgtgcaagtt ggggaggggc cccaacaaag agaaattgga
    10501 ttcccaattt ctacagacaa tgcaagttgg ggtgggacga cgaaatatat tttgcgaaaa
    10561 tatcatttct gtcccactcc ctatgcagct atccacttca tatgtatggc gcatacttga
    10621 taaatgaata tcatcaagat tttaaaagta tcgctaattt taaagtctta aaacgcatga
    10681 ttaatgtcat gatgtgttat tgcgttttgt ttttagagcc acgtatttcc taggacatat
    10741 agaggaacat tcaacgtaaa attcccgaaa attaatttta atcattctat aagtgaatgg
    10801 ttattttcag aagtgattac atttttacgt tacatgagta tttaactttc tatttcaaat
    10861 tacctagaat tatagcagct atcacgataa taataatacc tgcccaaata cccgtcatct
    10921 gacgacgatc ttttctttct cctaaaatga aaataccacc taatgttgaa acgataacaa
    10981 gtaactgtga taatgagaaa cttgtagcta caccaacttt tggttgagaa tagaacatga
    11041 acaagttacc aattccccaa atcacacctg gcaatagatt aagtgctgtt gatttaagtg
    11101 atgttttatg attcatggat aggataaagc caccaattgc cataccgaca gattggaaga
    11161 acaatgcatc agttccacca acaccaaaga tgtcaccaag tacaacgaaa cctacatatc
    11221 caactgtaga tacaattaaa atacccattg cttttttaaa ttcaggatta tctgattgac
    11281 gttcattttt ggctttaagt gaagtaagtg ctacaccagt aactaataat atcatggcga
    11341 ttaaaccaaa gataatttga gtcattgaag accattcacc taaaaagata acgctgaata
    11401 atgttgtacc aactaattgc atacctgtag aaactggcat agtatttgat acaccgatta
    11461 aactaataga tttaagctga ttcgcttgtc caaacgccca taatgcacca gaaataagac
    11521 cgacaataat tacagtagga ttattgaatt ttgcgaaacc agtaataagt aaacctaatc
    11581 caacaatgag tgcgcctagc gtcgtaccac gaatttggtt gtaaggtcca ccgccgacga
    11641 acacattaat aagaacgaca cttccccaga ataaagcagg taaaagtgcg attaagaaat
    11701 caagaaattg caagactact cactctccta tactattttt gcaagtgctc cccttaattg
    11761 acacttgcat gttattgtaa agcattctgc cctaatagta aaaaacaact acttataatt
    11821 ttcaatattt acaaaaagta gaaaaaccca caatacatgc taatgaagta ctgtggggta
    11881 tttgcttggt attttattca gggattgttt gatcttttac gttacgtgat tcgaatttga
    11941 atttaatgta ctcatagttg atatggaaac ctagaccatt aaatcgttcg atgagtttat
    12001 tatttttatt acgaatttta aaataaactt ttctaatatc aaaattatca aaagcatatt
    12061 gcaatgcata cgcaagcaac tcgaaagcga tacctttcaa acggtaatct acatgagaac
    12121 tgaagtattt aatctcggcg attgactgtt gtgaatcaat ttcaaggtat aaataacctt
    12181 taagtaaacc ttcgctaaca aataaaaaca aatgatgatg gtcgtctaaa ctatcaatga
    12241 tatcttgcgg tgactgtgcc tgatatttaa atgtagttgt gtgtaatttg ctgaaagcac
    12301 gataaaaccc tttgtgataa ggaatgatat ttggttggtg catatcttct tctaatcttg
    12361 tacgggcttc tatgtaatag tcagtgaagt tataacttgc ttgaataact ttcattaatg
    12421 gtttgtattg ttgaatgcct tcttcaaaag aaaagttaaa gacggcatcg tcaggttggt
    12481 tcgaagtcat tgctttaaat agcgtttcaa acgtatcgac agataataca taattgtcag
    12541 ccacgaatgg gcctatgact ttgaatttat ttttatcgta tgcaaaagac aatatgattg
    12601 ctttgattga atcgttttct ttaatcgaaa atacaccagg tgattcaatc gcatcggtta
    12661 ttaatacatc tatttcattg tactgttgag gaagtttata taagtatgat gctgattcat
    12721 aattcgaatg ttcaatgaac gattttattt gtgtaatatc atataactga actatgctca
    12781 tgtcattacc tccgtacttt ttgttacttt tattatatag tatttcaact gaaatgaaag
    12841 ttaatagtga tattaacatg ttacaataca tttaacacca ttgaatttaa atcaaagatt
    12901 agtggaatag atgaagcacg ttcgaaataa aagaacgtat gagaaaggat aatttatgaa
    12961 atctttaata ttagctgaaa aaccatcagt tgcaagagat attgctgatg ctttacaaat
    13021 aaatcagaag cgtaatggtt actttgaaaa taaccaatat attgtcacgt gggcgttagg
    13081 tcatctagtg acaaatgcga cacctgaaca atacgataaa aatttaaagg aatggcgatt
    13141 agaagacctt ccaattatac ctaaatatat gaaaactgtt gttattggta aaacaagcaa
    13201 acaatttaaa acagtaaaag cgttaatttt agataataaa gtgaaagata ttattattgc
    13261 aacagatgct ggacgagaag gtgaactagt tgcaagattg attttggata aagttggtaa
    13321 caaaaagcca atccgtcgat tatggattag ctcagttact aaaaaagcta ttcaacaagg
    13381 ttttaaaaat ttaaaagacg gtcgtcaata taacgatttg tattatgcag cgttagcgag
    13441 aagcgaggca gattggattg ttgggattaa tgcaacgcgt gcactaacaa caaagtatga
    13501 tgcacagcta tccctgggac gtgttcagac accaacgatt caattagtaa atacacgaca
    13561 acaagagatt aatcagttca aaccacaaca atactttaca ttatcattaa cggtaaaagg
    13621 gtttgatttt cagctagaat caaatcagcg atataccaat aaagaaactt tagaacagat
    13681 ggttaataat ttgaaaaatg tcgatggtaa gattaaatct gttgctacta aacataagaa
    13741 gtcgtatccg caatcactgt acaatttaac agatttacaa caagatatgt atagacgtta
    13801 taaaattgga cctaaagaaa cattgaatac acttcaaagc ttatatgaga gacataaagt
    13861 cgtaacctat ccaagaacag attcaaacta tttaacaact gatatggtag atactatgaa
    13921 agaacgtatt caggcgacga tggcaacaac atataaagac caagcacgcc cattaatgtc
    13981 taaaacattt tcatcaaaaa tgtcgatatt taataatcaa aaagtatctg atcaccatgc
    14041 aattattcct acagaagtga gacctgtcat gtcagactta agtaatagag aattaaagtt
    14101 atacgatatg attgtcgagc gttttttaga agctttaatg cctccgcacg agtatgacgc
    14161 gataactgta actttagagg ttgcagggca cacatttgtt ttgaaagaga atgtaacaac
    14221 tgttttaggt tttaaatcta ttagacaagg tgaatctatt acagagatgc aacagccttt
    14281 ttcagaaggc gatgaagtga agatttcaaa aacaaacatt agagaacatg aaacaacacc
    14341 tccagaatat tttaatgaag gttcgttatt aaaagcgatg gagaaccctc agaactttat
    14401 tcaattgaag gataaaaaat atgcgcaaac tttaaaacaa acaggtggta tcggcacagt
    14461 tgcaacaagg gccgacatta tcgataaatt atttaatatg aatgccattg aatcaagaga
    14521 cggtaaaatt aaagtaacgt caaaaggtaa acaaatatta gaattagcac cagaagaatt
    14581 aacgtcgcca cttttaactg cacaatggga agaaaaatta cttttaattg aacgtggtaa
    14641 atatcaggcg aaaacattta ttaatgaaat gaaagatttt acgaaagatg ttgtaaatgg
    14701 gattaaaaat agtgatcgta aatataaaca cgataattta acaaccacag aatgcccaac
    14761 gtgtggtaaa ttcatgatta aagttaaaac taaaaatggt cagatgcttg tgtgccaaga
    14821 tccatcttgt aagacgaaaa agaatgtaca gcgcaaaaca aatgcaagat gtccaaactg
    14881 taaaaagaaa ttaacgttgt ttggtaaagg gaaagaagcg gtatatcgtt gtgtttgtgg
    14941 acattctgaa acgcaagcac atatggatca gcgtatgaag tctaaatcct ctggtaaagt
    15001 atctcgtaaa gaaatgaaaa agtatatgaa taaaaatgaa ggtttagaca ataatccgtt
    15061 taaagatgca ttaaagaact tgaatttata gataaaatcg aacaaagttg aatcagaaaa
    15121 acgaaaagtt cgcttttggt attgtttttt attaagaatg atattaaact attaaggtat
    15181 tttaaaaaaa ggagcatcca ttcgtgaaaa actatttcca gttcgataaa tatggaacaa
    15241 actttaaaag agaaatctta ggcggtatca caactttctt atctatggcc tatattttag
    15301 cagttaaccc gcaagtttta agtttagcag gtgttaaagg cgtatcagaa gatatgaaaa
    15361 tggaccaagg tgccattttt gtagcgactg cattagcagc atttgtaggc tcgctattca
    15421 tgggactaat agctaaatat ccaatcgcat tagcaccagg tatgggatta aatgcattct
    15481 ttgcatttac tgttgtgtta accatgggta ttccttggca agttggttta acaggtgttt
    15541 tattctcagg tatattcttt gcgatattaa ccgtcacagg gtttagggaa gttattatta
    15601 atgcaattcc ttatcaaatg aagatggctg tttcagcagg tataggatta ttcattacat
    15661 ttgtaggttt acaaagttcg ggtattatag ttaaaaatga atctacatta gtaacattgg
    15721 gacatttaac tgatccacca gtacttttgg cgatattcgg tatcgttatt acagttattt
    15781 tatatgcaat taaattgccg ggttctatct ttatcggtat gatcatcaca gcaatcgttg
    15841 gtatgttcac tggattgatt caaatgccat ctggtattgt aggtaagatt ccaagtattg
    15901 aaccaacgtt cggtgctgca tttgaagcgt ttaaagaccc aagtcaatta ttaacgatac
    15961 agttcttaat tgttatttta acgttcttat ttattgattt ctttgataca gctggtacat
    16021 tagtagctgt tgctactcaa gcaggtatta tgaaagataa taaattacca agagctggta
    16081 gagcattgtt ctcagattct ttagctacga ttgtaggatc agtttttgga acaactacaa
    16141 caacttctta tatcgaatca acttcaggtg ttgctgtagg tgctagaaca ggatttgcta
    16201 gtatcgtaac aggttgttgt ttcctattag cgctgttctt tagtccgtta atcgctgttg
    16261 taacaagtgc agttacgaca ccagctttag tggtggtagg tgtattaatg gctgcaaact
    16321 ttgctgagat aaactggaaa agttttgaag ttgcagtgcc agcattcatt acaatcatta
    16381 tgatgccttt atcatactca attgcaactg gtattgcgtg tggatttatt ttctatccaa
    16441 ttacaatgct tatctctaaa aaacataaag aagtacatcc gataatgtat gtattaatgg
    16501 tactattcat tctgtacttc atatttgttc atggttaaat ccagacacaa aaaacgactg
    16561 ccaatgattg gtagtcgttt tttctttgaa gtaaggataa aataagaagc gaaataacat
    16621 aaagcaatat tatgaatgtc tttaaatatc cgatataaat atacttcttt taaaattgtt
    16681 ggacgtgttt cttctatatt atatacctaa ttaaaaaggt tttaagaatt attctctatg
    16741 tccatctttg tattgtgtgt aaatgacacg agcattttta ttaggtatat aggttggcgc
    16801 tgataataca actaggacaa atagaatcat taggaaaaat aaaatccaat gaatcaaaat
    16861 tcctaagaag gggatgatag ctaaaaaact acctactata ccaaatattg gaataatcgc
    16921 tataggtcta atcgcatttt gtctattcac taataatata atagtaagaa ttagataaag
    16981 taatgcatta aagagtaaag gctgccatcc aaaacttaaa ataaacgttg cacctaaaaa
    17041 aggaatgcca tagaaaaact cacaaagtat taaaaagata cctataagtg ctaaagataa
    17101 ttttggtcca tttttcatat tcattcacca ccgttcttat gactaattat atagaataat
    17161 tttcaatgat aataataaaa tgattcaagg tacgaattta caataaagaa ggaaataaaa
    17221 aatatctcga aaatagttga actgactaag tatataaagt aaaatataaa agtatgtgtt
    17281 agacagatag aaaaattatg tgaaaagcct tgatttatcg atgtttttcg tgtaatataa
    17341 ctaacgttgg actttaagaa aagcgatgaa gcgaaaggtt actgacacac ccggccgctt
    17401 tgccatggcg ctgtgtaaga tagttttcgt ggagaagtct atcactaaat gtagacgaat
    17461 aaggagggaa aattatggca aaacaaaaaa tcagaatcag attaaaagct tatgatcacc
    17521 gcgtgattga tcaatcagca gagaagattg tagaaacagc gaaacgttct ggtgcagatg
    17581 tttctggacc aattccgtta ccaactgaga aatcagttta cacaatcatc cgtgccgtgc
    17641 atatgtataa agattcacgt gaacaattcg aacaacgtac acacaaacgt ttaatcgata
    17701 ttgtaaaccc aacaccaaaa acagttgacg ctttaatggg cttaaactta ccatctggtg
    17761 tagacatcga aatcaaatta taatagacaa ttttaggagg tggactttcg atgaccaaag
    17821 gaatcttagg aagaaaaatt gggatgacac aagtattcgg agaaaacggt gaattaatcc
    17881 ctgtaacagt agtagaagct aaagaaaatg ttgtattaca aaagaaaact gtagaagttg
    17941 atggatacaa cgcaatccaa gttggatttg aagacaaaaa agcatacaaa aaagatgcaa
    18001 aatctaataa atatgctaat aaaccagctg aaggtcacgc taaaaaagct gacgcagcac
    18061 ctaagcgctt cattcgtgaa ttccgcaatg tagacgtgga tgcttacgaa gtaggtcaag
    18121 aagtctcagt agatactttt gtagctggcg acgttattga cgtaacaggc gtatcaaaag
    18181 gtaaaggttt ccaaggtgca attaaacgcc acggacaatc tcgtggacct atgtcacacg
    18241 gttctcattt ccacagagca ccaggttctg taggtatggc ttcagatgct tctagagtat
    18301 ttaaaggcca aaaaatgcca ggacgtatgg gtggaaacac tgtaactgtt caaaacttag
    18361 aagtagttca agttgacaca gaaaacaaag ttatcttagt aaaaggtaac gtacctggac
    18421 ctaaaaaagg tttagtagaa atcagaactt caattaaaaa aggtaataaa taataagaag
    18481 tgaaaggagg aaatgcataa tggctaatta tgatgtttta aaattagacg gaactaaatc
    18541 aggttcaatc gaattaagcg atgcagtatt cggtattgag ccaaataata gcgttttatt
    18601 cgaagctatt aatttacaac gtgcttcatt acgtcaaggt acgcatgctg ttaagaatcg
    18661 ttcagcagta agcggtggcg gacgtaaacc atggaagcaa aaaggaacag gtcgtgctcg
    18721 tcaaggtaca atccgtgctc cacaatggcg tggcggtggt atcgtattcg gaccaactcc
    18781 aagaagttat gcatacaaaa tgcctaagaa aatgcgtcgt ttagctttac gctcagcatt
    18841 atctttcaaa gctcaagaga atggcttaac tgtagttgac gcattcaact tcgaagctcc
    18901 aaaaactaaa gaattcaaaa atgtattatc tacattagaa caacctaaaa aagtattagt
    18961 agttactgaa aacgaagatg taaatgttga attatcagca cgcaacatcc ctggcgttca
    19021 agtgacaact gctcaaggtt taaatgtttt agatatcact aatgctgaca gcttagtaat
    19081 tactgaagct gctgctaaaa aagttgagga ggtgctcgga taatggaagc aagagatatt
    19141 cttaagcgcc ccgtaatcac tgagaaatct tctgaagcaa tggctgaaga caaatacact
    19201 ttcgacgttg atactcgtgt taacaaaaca caagtaaaaa tggcagttga agaaatcttc
    19261 aacgtaaaag ttgcaagtgt taatatcatg aattacaaac ctaagaaaaa acgtatgggc
    19321 cgttaccaag gctatacaaa caaaagaaga aaagcgattg taactcttaa agaaggatca
    19381 atcgacttat ttaactaata aaacaattac cattaaggag gtaagcgaca atggctatta
    19441 aaaagtataa gccaataaca aatggtcgtc gtaatatgac ttcgttagat ttcgcagaaa
    19501 tcacgaaaac tacacctgaa aagtcattat taaaaccgct accgaaaaaa gcgggacgta
    19561 acaaccaagg taaattgact gtaagacacc atggtggtgg acacaaacgt caataccgtg
    19621 ttatcgattt taaacgtaac aaagatggta tcaatgcaaa agttgattct attcaatatg
    19681 atccaaaccg ctcagcaaac atcgctttag ttgtatatgc agacggtgaa aaacgatata
    19741 tcattgctcc taaaggatta gaagtaggtc aaatcgttga aagtggtgct gaagctgaca
    19801 tcaaagttgg taacgcatta ccattacaaa acattccagt tggtacagta gtacacaaca
    19861 tcgagcttaa acctggtaaa ggtggacaaa tcgctcgttc agctggtgca agtgctcaag
    19921 tacttggtaa agaaggtaaa tacgtattaa tcagattaag atctggtgaa gttcgtatga
    19981 tcttatctac ttgccgtgct acaatcggtc aagttggtaa cctacaacac gaattagtta
    20041 acgttggtaa agccggacgt tcaagatgga aaggtatccg tccaacagtt cgtggttctg
    20101 taatgaaccc taacgatcac ccacacggtg gtggtgaagg tcgtgctcct atcggtagac
    20161 catctccaat gtcaccatgg ggtaaaccta cgcttggtaa gaaaactcgt cgtggtaaaa
    20221 aatcatcaga caaacttatc gttcgtggac gtaagaaaaa ataatataat caacttattt
    20281 gggtgtgcgg cttaaagctg cacgcacata ataagaaggg aggcgcccaa atggctcgta
    20341 gtattaaaaa aggacctttc gtcgatgagc atttaatgaa aaaagttgaa gctcaagaag
    20401 gaagcgaaaa gaaacaagta atcaaaacat ggtcacgtcg ttctacaatt ttccctaatt
    20461 tcatcggaca tacttttgca gtatacgacg gacgtaaaca cgtacctgta tatgtaactg
    20521 aagatatggt aggtcataaa ttaggtgagt ttgctcctac tcgtacattc aaaggacacg
    20581 ttgcagacga caagaaaaca aaaagataat atctattaag tagaggagga catcctaatg
    20641 gaagcaaaag cggttgctag aacaataaga atcgcacctc gtaaagtaag actagttctt
    20701 gacttaatca gaggtaaaaa tgctgctgaa gctattgcaa ttttaaaatt aacaaacaaa
    20761 gcttcatcac cagtaattga aaaagtatta atgtccgctt tagctaatgc tgaacataac
    20821 tatgacatga acacagatga attagtagtt aaagaagcat atgctaacga aggaccaaca
    20881 ttaaaacgtt tccgtccacg tgcgcaaggt cgtgcaagtg cgattaacaa acgtacaagc
    20941 cacattacaa tcgtcgtaag tgacggtaaa gaagaagcta aagaagctta attaactttt
    21001 aaggagggaa tactgtgggt caaaaaatta atccaatcgg acttcgtgtt ggtattatcc
    21061 gtgattggga agctaaatgg tatgctgaaa aagacttcgc ttcactttta cacgaagatt
    21121 taaaaatccg taaatttatt gataatgaat taaaagaagc atcagtttct cacgtagaga
    21181 ttgaacgtgc tgcaaaccgt atcaacattg caattcatac tggtaaacct ggtatggtaa
    21241 ttggtaaagg cggttcagaa atcgaaaaat tacgcaacaa attaaatgcg ttaactgata
    21301 aaaaagtaca catcaacgta attgaaatca aaaaagttga tcttgacgct cgtttagtag
    21361 ctgaaaacat cgcacgtcaa ttagaaaacc gtgcttcatt ccgtcgtgta caaaaacaag
    21421 caatcactag agctatgaaa cttggtgcta aaggtatcaa aactcaagta tctggtcgtt
    21481 taggcggagc tgacatcgct cgtgctgaac aatattcaga aggaactgtt ccacttcata
    21541 cgttacgtgc tgacatcgat tatgcacacg ctgaagctga cactacttac ggtaaattag
    21601 gcgttaaagt atggatctat cgtggagaag ttcttcctac taagaacact agtggaggag
    21661 gaaaataata atgttactac caaaacgtgt aaaatatcgt cgtcaacatc gtcctaaaac
    21721 aactggtcgt tctaaaggcg gtaactacgt aacatttggt gagtttggtt tacaagctac
    21781 aacaacgtct tggatcacat ctcgtcaaat cgaatctgct cgtatagcaa tgacacgtta
    21841 catgaaacgt ggcgggaaag tttggattaa aatcttccca catacaccat atactaaaaa
    21901 acctttagaa gtacgtatgg gtgctggtaa aggtgcggtt gaaggctgga tcgcagttgt
    21961 taaaccaggt agaattttat tcgaagttgc tggcgtttct gaagaagttg cgcgtgaagc
    22021 actacgttta gcaagtcaca aacttccagt aaaaactaag tttgtaaaac gtgaggaatt
    22081 gggtggtgaa acaaatgaaa gctaaggaaa ttagagactt aaccacttca gaaatcgaag
    22141 aacaaatcaa atcttcaaaa gaagagcttt ttaacctacg ctttcagtta gctacaggtc
    22201 aattagaaga aactgcacgt attcgtacag taagaaaaac gattgcacgt ctaaaaactg
    22261 ttgctcgtga aagagaaatt gaacaaagta aggctaatca ataattaagt aagaggaggt
    22321 tacaaaagtg agcgaaagaa acgatcgtaa agtttatgta ggtaaagttg tttcagacaa
    22381 aatggacaag actattacag tacttgttga aacttacaaa acacacaaat tatacggtaa
    22441 acgagtaaaa tactctaaaa aatacaaaac tcatgatgaa aacaattcag ctaaattagg
    22501 agacattgtt aaaattcaag aaactcgtcc tttatcagca acaaaacgtt ttcgtatagt
    22561 agagattgtt gaagagtcag taattattta atacaagttt agagataagg gaggtttaac
    22621 taatgatcca acaagaaaca cgcttgaaag tagcagacaa ctctggtgct cgtgaagttc
    22681 ttacaatcaa agtattaggt ggatctggtc gtaaaacagc aaacatcggc gatgttatcg
    22741 tatgtactgt taaaaatgca acaccaggtg gcgttgttaa aaaaggtgac gttgtcaaag
    22801 ctgtaatcgt acgtactaag tcaggtgttc gtcgtaatga cggttcatac atcaaatttg
    22861 atgaaaatgc atgtgttatc atccgtgatg acaaaggccc acgtggtact cgtatcttcg
    22921 gacctgttgc tcgtgaatta cgtgaaggta acttcatgaa aatcgtatca ttagcaccag
    22981 aagtacttta attaataaca accaatacaa ataaggaggt gcccacatgc atatcaaaaa
    23041 aggtgacaac gttaaagtta tcgcaggtaa agacaaaggt aaagaaggta aagtaattgc
    23101 tactctacct aaaaaagacc gtgtcgttgt ggaaggtgtt aacattatga aaaaacacca
    23161 aaaaccaact caattaaatc ctgaaggtgg aatcttagaa acagaggcag caatccatgt
    23221 ttctaatgta caattattgg accctaaaac aaacgaacca actcgtgtag gttacaaatt
    23281 tgttgatggt aaaaaagttc gtatcgctaa aaaatctggc gaagaaatta aatctaataa
    23341 ttaataacaa gttgaaagga ggatcccact ttgaaccgtt taaaagaaaa gtttaacact
    23401 gaagttactg aaaacttaat gaaaaaattc aattatagtt cagtaatgga agtaccaaaa
    23461 atagataaaa tcgttgtgaa catgggtgta ggtgacgcag tacaaaattc taaagtatta
    23521 gacaatgctg ttgaagaatt agaattgatc actggtcaaa aaccattagt aactaaagct
    23581 aaaaaatcaa tcgcgacttt ccgtttacgt gaaggtatgc caatcggtgc gaaagtaaca
    23641 cttcgcggtg aaagaatgta tgaattctta gacaaattaa tttcagtatc attaccacgt
    23701 gtacgtgact tccaaggtgt ttctaaaaaa gcatttgacg gacgcggtaa ctacacttta
    23761 ggtgttaaag aacaattaat tttcccagaa atcgactatg ataaagtaag taaagttaga
    23821 ggaatggata ttgttatcgt aacgactgct aacactgatg aagaagctcg tgaattgtta
    23881 gctaacttcg gtatgccatt ccgtaaataa ttatttaaag gaggctaatt aagtggctaa
    23941 aacttcaatg gttgctaagc aacaaaaaaa acaaaaatat gcagttcgtg aatacactcg
    24001 ttgtgaacgt tgtggccgtc cacattctgt atatcgtaaa tttaaattat gccgtatttg
    24061 tttccgtgaa ttagcttaca aaggccaaat ccctggcgtt cgtaaagcta gctggtaata
    24121 aaaaagagtc tgaaaggagg caacaatcaa tgacaatgac agatccaatc gcagatatgc
    24181 ttactcgtgt aagaaacgca aacatggtgc gtcacgagaa gttagaatta cctgcatcaa
    24241 atattaaaaa agaaattgct gaaatcttaa agagtgaagg tttcattaaa aatgttgaat
    24301 acgtagaaga tgataaacaa ggtgtacttc gtttattctt aaaatatggt caaaacgatg
    24361 agcgtgttat cacaggatta aaacgtattt caaaaccagg tttacgtgtt tatgcaaaag
    24421 ctagcgaaat gcctaaagta ttaaatggtt taggtattgc attagtatca acttctgaag
    24481 gtgtaatcac tgacaaagaa gcaagaaaac gtaatgttgg tggagaaatt atcgcatacg
    24541 tttggtaata aaaaataagg aggtgccata acatgagtcg tgttggtaag aaaattattg
    24601 acatccctag tgacgtaaca gtaacttttg atggaaatca tgtaactgtt aaaggtccta
    24661 aaggtgaatt atcaagaact ttaaatgaaa gaatgacatt caaacaagaa gaaaacacaa
    24721 ttgaagttgt aagaccatct gattctaaag aagatagaac aaaccatggt acaactcgtg
    24781 ctttattaaa caatatggta caaggtgttt ctcaaggata cgtaaaagta cttgaacttg
    24841 ttggtgtagg ttaccgtgct caaatgcaag gtaaagactt aatccttaac gttggttatt
    24901 ctcacccagt agaaattaaa gctgaagaaa acattacttt ctcagttgag aaaaacacag
    24961 tcgttaaagt tgaaggtatt tcaaaagaac aagttggagc attagcatct aacatccgtt
    25021 cagtaagacc tccagagcct tacaaaggta aaggtattcg ttaccaaggt gaatacgttc
    25081 gccgtaaaga aggtaaaact ggtaaataat agataaactc taaagaaagg agtattgaaa
    25141 tgatcagtaa aattgataaa aataaagtgc gtttaaaaag acatgctcgt gttcgtacta
    25201 acttatcagg tacagctgaa aagccacgtt taaacgtata tcgttcaaac aagcatatct
    25261 acgctcaaat tattgatgat aataaaggcg taacattagc tcaagcttct tcaaaagaca
    25321 gcgacattgc tactacagca actaaagttg aattagcaac taaagtcggt gaagcaattg
    25381 ctaaaaaagc tgctgacaaa ggcattaaag aaatcgtatt tgaccgtgga ggatatttat
    25441 atcacggacg tgttaaagca ttagctgaag cagcaagaga aagcggatta gaattttaat
    25501 ttaaaggagg gacaaataca tggctcgtag agaagaagag acgaaagaat ttgaagaacg
    25561 cgttgttaca atcaaccgtg tagcaaaagt tgtaaaaggt ggtcgtcgtt tccgtttcac
    25621 tgcattagtt gtagttggag acaaaaatgg tcgtgtaggt ttcggtactg gtaaagctca
    25681 agaggtacca gaagcaatca aaaaagctgt tgaagcagct aaaaaagatt tagtagttgt
    25741 tccacgtgtt gaaggtacaa ctccacacac aattactggc cgttacggtt caggaagcgt
    25801 atttatgaaa ccggctgcac ctggtacagg agttatcgct ggtggtcctg ttcgtgccgt
    25861 acttgaatta gcaggtatca ctgatatctt aagtaaatca ttaggatcaa acacaccaat
    25921 caacatggtt cgtgctacaa tcgatggttt acaaaacctt aaaaatgctg aagatgttgc
    25981 gaaattacgt ggcaaaacag tagaagaatt atacaattaa ggagggaaaa ctagttatgg
    26041 ctaaattaca aattaccctc actcgtagtg ttattggtcg tcctgaaaca caacgtaaaa
    26101 ctgttgaagc tttaggtctt aaaaagacta acagttcagt agttgttgaa gataaccctg
    26161 ctattcgtgg gcaaatcaac aaagttaagc acttagtaac agtagaagaa aaataatcaa
    26221 ggaggtgccg aaatgaaatt acatgagtta aaaccggcag aaggttcacg taaagaacgc
    26281 aatcgtgttg gacgtggtgt tgcgacaggt aatggtaaaa caagtggtcg cggacacaaa
    26341 ggtcaaaaag ctcgttcagg cggtggtgta agaccaggat ttgaaggtgg tcaattacca
    26401 ttattccgtc gtttaccaaa acgtggtttt actaacataa atcgtaaaga atatgctatt
    26461 gttaacttag accaacttaa taaatttgaa gatggtactg aagtaactcc agctttatta
    26521 gtagaatctg gtgttgttaa gaatgaaaaa tctggtatca aaatactagg taatggttca
    26581 cttgataaga aattgacagt gaaagctcat aaattctcag cttcagcagc agaagctatt
    26641 gatgctaaag gtggagcaca cgaggtgatc taatgattca aacccttgtg aacttcttta
    26701 gaacaaaaga ggttcgtaac aagattttct tcacactagc aatgttagta atttttaaaa
    26761 tagggactta tataccagct ccaggagtaa atcctgcagc ttttgataat ccccaaggtt
    26821 ctcaaggtgc cactgagtta ttaaatactt ttggtggcgg agccttgaaa cgattttcta
    26881 tttttgcaat gggtattgta ccctacatca ctgcatcaat cgtaatgcaa ttattacaaa
    26941 tggatattgt ccctaaattc tcagaatggg caaaacaagg tgaagtaggt agaagaaagt
    27001 taaataacgt tactcgttat ttagcaattt ctttagcatt tatccaatct ataggtatgg
    27061 cattccaatt taataattat ctcaaaggtg cgctgattat caatcagtca attatgagtt
    27121 atttattaat agcactagtt ttgacagcag gaactgcttt cttaatatgg cttggtgatc
    27181 aaatcactca gttcggtgtt ggtaatggta tttctattat catatttgcg ggtattttat
    27241 caacgttgcc agccagtcta attcaatttg gacaaactgc gttcgttggg caagaagata
    27301 catcattagc atggttgaaa gtattaggac ttttagtgtc actgatacta ttaactgtag
    27361 gtgctattta tgtacttgaa gccgttcgta aaattccgat tcaatatgct aagaaacaaa
    27421 ctgctcaaag attaggttca caggcaactt atctaccttt aaaagtgaac tcagccggtg
    27481 ttattcctgt aatctttgca atggcgttct tcttactgcc tagaacatta acgttgttct
    27541 atccagataa agaatgggca caaaacattg cgaatgctgc aaatccttca agtaatgttg
    27601 gtatggttgt atatattgta ctaattatac tattcacata cttctatgca tttgtacaag
    27661 ttaatccgga aaaaatggct gataacctta agaaacaagg tagctatgtt ccaggcatta
    27721 gacctggtga acaaaccaaa aaatatatta ctaaagtttt atatcgttta acttttgtag
    27781 gttctatctt cttagccgtt atttcaattc taccaatatt agcaactaag tttatgggat
    27841 taccacaatc aattcagatt ggtggtacaa gtttacttat cgttattggt gtagcgattg
    27901 aaacaatgaa atctttagaa gctcaagtat ctcaaaaaga atataaaggc tttggtggta
    27961 gataatttta ggagggcact tatgaatatc attttgatgg gtttacctgg cgcaggtaaa
    28021 ggaactcaag caagtgaaat tgtcaagaaa ttcccaatac cccacatttc aactggtgac
    28081 atgttcagaa aagctataaa agaagaaact gaattaggta aagaagctaa gtcttatatg
    28141 gaccgtggcg aattagttcc tgatgaagtg actgtaggta tcgttaagga aagaatttct
    28201 gaagacgatg caaaaaaagg ctttttatta gatggcttcc caagaacaat cgagcaagct
    28261 gaggcattaa ataatattat gtctgagctt gacagaaaca ttgatgctgt catcaatatc
    28321 gaagttccgg aagaagaatt aatgaaccgt cttacaggtc gtcgaatctg tgagtcatgt
    28381 ggtacaacgt atcatcttgt atttaatcct ccgaaggtcg aaggtatttg tgatatcgat
    28441 ggtggtaaat tgtatcaacg agaagatgat aatcctgaaa cggtagctaa tcgtttgagt
    28501 gttaatatta aacaatctaa gcctatttta gatttctatg atcaaaaagg tgtattgaaa
    28561 aatattgatg gttcaaaaga tattagcgac gttaccaaag atgtcattga tattttagat
    28621 catttgtaat agatcaactt ttagcggtct attttaagtg aggcatgatt taatcatatt
    28681 agatagatga gagtggcaat taacgataat taattcccgt aatttttgtt taacttataa
    28741 aattaatcac tgatagattc gtaacaataa agttgtctat tgacgattac cttactattg
    28801 caaaaagggg gaagttaatc aatggctaaa caagatgtaa ttgaattaga aggtactgta
    28861 ttagatactt taccgaacgc aatgtttaaa gtagaattag aaaatggtca tgagatttta
    28921 gctcacgtaa gtggtaaaat cagaatgaat tacattcgta ttctacctgg cgacaaagta
    28981 actgttgaga tgtctccgta cgatttaaca cgcggaagaa ttacttatcg ttataaataa
    29041 tcgtcactcc ataatatagg gaggtataaa aatgaaagta agaccatcag taaaacctat
    29101 ttgcgaaaaa tgtaaagtca ttaaacgtaa aggtaaagta atggtaattt gtgaaaatcc
    29161 aaaacacaaa caaagacaag gttaataaaa gagaggtgta aattaatatg gcacgtattg
    29221 caggagtaga tattccacgt gaaaaacgcg tagttatctc attaacttat atatacggta
    29281 tcggtacgtc aactgctcaa aaaattcttg aagaagctaa cgtatcagct gatactcgtg
    29341 tgaaagattt aactgatgac gaattaggtc gcatccgtga agttgtagac ggttataaag
    29401 tcgaaggtga cttacgtcgt gaaactaact taaatatcaa acgtttaatg gaaatttcat
    29461 cataccgtgg tatccgtcac cgtcgtggtt taccagttcg tggtcaaaaa acgaaaaaca
    29521 acgcgcgtac tcgtaaagga ccagttaaaa cggtagctaa caagaaaaaa taataggtaa
    29581 aggaggcaaa ttttaaatgg cacgtaaaca agtatctcgt aaacgtagag tgaaaaagaa
    29641 tattgaaaat ggtgtagcac acatccgttc aacattcaac aacactattg taactatcac
    29701 tgatgagttc ggtaatgctt tatcatggtc atcagctggt gcattaggat tcaaaggatc
    29761 taaaaaatca acaccatttg cagcacaaat ggcttctgaa actgcatcta aatcagctat
    29821 ggagcatggt ttaaaaacag ttgaagtaac agttaaagga cctggtccag gtcgtgaatc
    29881 agctattcgt gcattacaat ctgcaggttt agaagtaact gcgatcagag acgttactcc
    29941 agtacctcat aacggttgtc gtccaccaaa acgtcgtcgt gtataattta tgatggtatt
    30001 gttacaggtc actgagcaaa cattttaaat taagtcgacg tatataagga ggatatttaa
    30061 atgatagaaa tcgaaaaacc tagaattgag acaattgaaa ttagtgaaga tgctaaattc
    30121 ggtaagttcg ttgttgaacc actagaacgt ggctacggta ctacactagg aaactcctta
    30181 cgtcgtatcc tactatcttc attaccaggt gcagccgtta agtatattga aattgaggga
    30241 gttttacatg aattctcagc agtagacaat gtagttgaag atgtttctac aattattatg
    30301 aacattaaac aattagcatt gaaaatttac tctgaagaag ataaaacttt agaaattgat
    30361 gtacgtgatg aaggcgaagt aacagcaagc gacattacac atgatagtga tgttgaaatt
    30421 ttaaacccag agcttaaaat tgcaacagta tctaaaggtg gtcacttaaa aattcgtcta
    30481 gttgctaaca agggtagagg ttacgcatta gcagaacaaa ataatactag tgatttacca
    30541 attggtgtaa tccctgttga ttcattgtat tcacctgttg aacgtgtgaa ctatactgtt
    30601 gaaaatacac gtgtaggtca aagcagtgat tttgataaat taacattgga tgtttggact
    30661 aatggttcaa tcacaccaca agaatcagtt tcattagcag caaaaataat gactgaacac
    30721 ttgaatatct tcgttggtct tactgatgaa gcgcaaaacg ctgaaatcat gattgaaaaa
    30781 gaagaagatc aaaaagaaaa agtattagaa atgtctattg aagaattaga cttatctgta
    30841 cgttcatata actgcttaaa acgcgcagga atcaattctg ttcaagagtt agctgacaaa
    30901 tctgaagctg acatgatgaa agtgcgtaat ttaggtcgta aatctttaga agaagttaaa
    30961 tacaaattag aagatttagg attaggatta agaaaagaag attgataaag gaggttaact
    31021 catgggttac agaaaattag gtcgtacttc tgatcaacgt aaagctatgt tacgtgactt
    31081 agctacatca cttattatta gtgaacgtat tgaaactaca gaagctcgtg caaaagaagt
    31141 tcgcagtgtt gttgagaaat taatcacttt aggtaaaaaa ggagatttag cttctcgtcg
    31201 taatgcagct aaaactttac gtaatgttga aatcttaaac gaagatgaaa ctacacaaac
    31261 tgcacttcaa aaattatttg gtgaaatcgc agagcgttac acagaacgtc aaggtggtta
    31321 cactcgtatc cttaaacaag gccctcgtcg cggtgacggt gctgaatcag taattatcga
    31381 attagtataa gaatttatat aaatacacta actactttta tataaagcaa atgagtgcaa
    31441 cgataatgtt tgccacatac aaatattgtc tagctcagag taccccatca actaaataaa
    31501 gatttaaagc gtgaactcaa caaacttatg atggggtgcg cgctttttta ttgaataaga
    31561 tatcggactt atgaacagaa atgtttttaa gtccgatttc tttatatcta cttttttgag
    31621 tgcgaatcta aaaatatgat caagctagtt tgatcaattt cactagaact gaatgaagtg
    31681 aaagtattat cgttgaacca aacgcgacca cccctatatt agtaaatcat ttattttatt
    31741 aacgtttata cataattgtt agagaggaat aaatgtcctt atcattagtt gttaaacaac
    31801 actttaaaga ctagaaatga gttagaaaat actagtaata tatagcaaac ccagtcaatg
    31861 tcatatacag cgatttataa atattgtaaa atatgataga tacacgaatc gtaaaggaga
    31921 gagacaagtg gaggataaga attcagttat tgtatttaaa aatgtttcat ttcaatatca
    31981 aagtgatgca tccttcacat tgaaagatgt ttcttttaat atacctaaag gtcagtggac
    32041 atctattgtt ggtcataacg gttctggaaa atctacaatt gccaagttaa tgattggcat
    32101 agagaaagtt aaatctggag aaatttttta taataatcaa gctataactg atgataattt
    32161 tgaaaagtta agaaaagaca taggaattgt atttcagaat ccggataatc aatttgttgg
    32221 ttcaattgta aaatacgatg tggcatttgg actcgaaaat catgcggttc cacatgacga
    32281 aatgcataga agagtcagcg aagcacttaa acaagttgat atgttagaac gtgcagatta
    32341 tgaacctaat gcattatcgg ggggacagaa gcagcgtgtg gctatagcaa gtgtattagc
    32401 acttaacccc tctgtcatta tattagatga ggcgacttct atgttagatc ctgatgcacg
    32461 tcaaaattta ttggatttag tgagaaaagt taaatcagaa cataatatta caatcatttc
    32521 tattacgcat gatttatctg aggcgatgga agcagatcat gttatcgtta tgaataaagg
    32581 gactgtctat aaagaaggca cagcgactga aattttcgac catgcagaag agttaacaag
    32641 aataggtcta gatttgccat tcccaatcaa aataaatcaa atgctgggac accaaacatc
    32701 attcttaact tatgaagggc tggtggatca actatgacta tacggtttga caatgtaagt
    32761 tatacctatc aaaaagggac accatatcag catcaagcta ttcatgatgt taatacagaa
    32821 tttgaacaag gtaaatatta cgccatcgtt ggacaaacgg gtagtggtaa atcaacgttg
    32881 atacaaaata ttaatgcgct gttaaagccg actactggga cagttacagt tgatgacatt
    32941 actattacac ataagaccaa agataaatat attagacctg taagaaaaag aattggaatg
    33001 gtatttcaat ttcccgaatc tcaattattt gaggacacag tagagcgtga aatgatattt
    33061 ggacctaaaa actttaaaat gaatttagat gaagccaaaa actatgccca tcgtctgttg
    33121 atggatttag gcttttcaag agatgtaatg tcacaatcac catttcaaat gtcaggtgga
    33181 caaatgcgta aaatagcgat tgtatcgata ttggcaatga atcctgatat tatcgtggtt
    33241 gatgaaccta cagcaggact tgatccacaa agtaaacgac aagtaatgag attactaaag
    33301 tcactacaaa cagatgaaaa taaggcaatt atcctaattt cacatgatat gaatgaagtc
    33361 gcgcgttatg cagatgaagt catcgttatg aaagaaggta gtatagtgtc gcaaacatca
    33421 ccaaaagagc tcttcaaaga taaaaaaaaa ttggcggatt ggcatattgg tttgccagaa
    33481 attgttcagt tacaatatga ctttgaacaa aaatatcaaa caaaattaaa agatattgcc
    33541 ttaactgaag aagcatttgt aagcttgtat aaggagtggc aacatgaaaa ataaattgat
    33601 agcaaaatct ttattaacaa tagcggcaat tggtattact acaactacaa ttgcgtcaac
    33661 agcagatgcg agcgaaggat acggtccaag agaaaagaaa ccagtgagta ttaatcacaa
    33721 tatcgtagag tacaatgatg gtacttttaa atatcaatct agaccaaaat ttaactcaac
    33781 acctaaatat attaaattca aacatgacta taatatttta gaatttaacg atggtacatt
    33841 cgaatatggt gcacgtccac aatttaataa accagcagcg aaaactgatg caactattaa
    33901 aaaagaacaa aaattgattc aagctcaaaa tcttgtgaga gaatttgaaa aaacacatac
    33961 tgtcagtgca cacagaaaag cacaaaaggc agtcaactta gtttcgtttg aatacaaagt
    34021 gaagaaaatg gtcttacaag agcgaattga taatgtatta aaacaaggat tagtgagata
    34081 ata
//
LOCUS       DANBWH010000005        34001 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.005, whole genome shotgun sequence.
ACCESSION   DANBWH010000005 DANBWH010000000
VERSION     DANBWH010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 34001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34001
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.005"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            177..2117
                     /gene="ltaS"
                     /locus_tag="PPY92_000172"
     CDS             177..2117
                     /gene="ltaS"
                     /locus_tag="PPY92_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyglycerol-phosphate lipoteichoic acid
                     synthase LtaS"
                     /protein_id="HDJ2624204.1"
                     /translation="MSSQKKKISLFAFFLLTVITITLKTYFSYYVDFSLGVKGLVQNL
                     ILLMNPYSLVALVLSVFLFFKGKKAFWFMFIGGFLLTFLLYANVVYFRFFSDFLTFST
                     LNQVGNVESMGGAVSASFKWYDFVYFIDTLVYLFILIFKTKWLDTKAFSKKFVPVVMA
                     ASVALFFLNLAFAETDRPELLTRTFDHKYLVKYLGPYNFTVYDGVKTIENNQQKALAS
                     EDDLTKVLNYTKQRQTEPNPEYYGVAKKKNIIKIHLESFQTFLINKKVNGKEVTPFLN
                     KLSSGKEQFTYFPNFFHQTGQGKTSDSEFTMDNSLYGLPQGSAFSLKGDNTYQSLPAI
                     LDQKQGYKSDVMHGDYKTFWNRDQVYKHFGIDKFYDATYYDMSDKNVVNLGLKDKIFF
                     KDSANYQAKMKSPFYSHLITLTNHYPFTLDEKDATIEKSNTGDATVDGYIQTARYLDE
                     ALEEYINDLKKKGLYDNSVIMIYGDHYGISENHNNAMEKLLGEKITPAKFTDLNRTGF
                     WIKIPGKSGGINNEYAGQVDVMPTILHLAGIDTKNYLMFGTDLFSKGHNQVVPFRNGD
                     FITKDYKYVNGKIYSNKNNELITTQPADFEKNKKQVEKDLEMSDNVLNGDLFRFYKNP
                     DFKKVNPSKYKYETGPKANSKK"
     gene            2387..4270
                     /locus_tag="PPY92_000173"
     CDS             2387..4270
                     /locus_tag="PPY92_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDJ2624205.1"
                     /translation="MGMEAYKIEHLNKSYADKTIFDNLDLSISEGEKIGLVGINGTGK
                     STLLKVIGGIDDDFTANVMHPNQYRIRYSSQKQDLNEDMTVFDAVLSSDTTTLRIIKQ
                     YEQAVQAYADDQSDKLFKRMIDAQDAMDQHDAWDYNAEIKTILSKLGIHDTTKYIKEL
                     SGGQQKRVVLAKTLIEQPDLLLLDEPTNHLDFESISWLINYVKQYPHTVLFVTHDRYF
                     LNEVSTRIIELNRGKLASYPGNYESYIEMRAEREVTLQKQQQKQRALYKEELAWMRAG
                     AKARTTKQQARINRFNDLENEVNQQYKDDKGELNLAYSRLGKQVFELEDLSKAINDKV
                     LFEHLTEIIQKGERIGVVGPNGAGKTTLLNILSGEDQQFEGKLKTGQTVKVAYFKQTD
                     ETLDRDIRMIDYLREESEVAKEKDGTSVSITQLLERFLFPSATHGKKVYKLSGGEQKR
                     LYLLRLLVHQPNVLLLDEPTNDLDTETLTILEDYIHTFGGTVITVSHDRYFLNKVAQS
                     YWFIHDGQMEKIIGTFEDYESYKKSLDKNKSTLKQQSKSYTTVRKKNGLSYKEKLEYE
                     QLMKRIEQAEVRMEEIDVLMIEASADYGKIKELNEEKEQLEIQYDLDITRWSELEEIK
                     EQQ"
     gene            4282..6063
                     /gene="recQ"
                     /locus_tag="PPY92_000174"
     CDS             4282..6063
                     /gene="recQ"
                     /locus_tag="PPY92_000174"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDJ2624206.1"
                     /translation="MMQQTLSHYFGYETFRPGQEEIISKVLDHRNVLGVLPTGGGKSI
                     CYQVPGLLLGGTTIVISPLISLMKDQVDQLKAMGIQAAFLNSSLTQKEQQRIEKALSN
                     GEIQFLYVAPERFENRYFLNMLQRIKIHLVAFDEAHCISKWGHDFRPSYQNVISKVFT
                     LPQDFTIIALTATATVEVQQDIREKLNIAQTDQIKTSTKRRNLIFKVNPTYQRQKFIL
                     DYIKTHDEDAGIIYCSTRKQVEELQEALESQKIESVIYHAGLSNKEREEAQNDFLFDR
                     VKVVVATNAFGMGIDKSNVRFVIHYNMPGDLESYYQEAGRAGRDGLKSECILLFSERD
                     INLHEYFITVSQADDDYKDKMGEKLTKMIQYTKTKKCLEATIVHYFEPNEKLEECEQC
                     SNCVQQDKSYNMTQEAKMIISCIARMKQQESYSVIIQVLRGESTDYIKYKGYDQISTH
                     GLMKGYTTSELSHLIDELRFKGFLNENDEILMCDTSIKKLLSNEVEVFTTPFKQKATE
                     KVFINTVEGVDRVLFSQLVEVRKKLSDKLTIAPVSIFSDYTLEEFAKRKPASKQDMIN
                     IDGVGSYKLKHYCPAFLETIQNYKAKV"
     gene            6285..7262
                     /locus_tag="PPY92_000175"
     CDS             6285..7262
                     /locus_tag="PPY92_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDJ2624207.1"
                     /translation="MIKFKNVTKRYGKHVAVDNISFNINEGEFFVLIGPSGCGKTTTL
                     KMINRLIHLSEGYIYFKDKPISDYPVYEMRWDIGYVLQQIALFPHMTIKENIAQVPQM
                     KKWKEKDIDKRVDELLDMVGLEPEKYKNRKPDELSGGQRQRVGVIRALAADPPVILMD
                     EPFSALDPISREKLQDDLIELQTKIKKTIIFVTHDIQEAMKLGDKICLLNEGHIEQID
                     TPEGFKNNPQSEFVKQFMGSHLEDDAPCVEENAIIRDLDIMKPIDEVTSMSAYPIVYD
                     NQPIEVLYQHLSESERVIVMQEDSVGQYVIDRKDIFKYLSQKKEVAQHD"
     gene            7255..8769
                     /locus_tag="PPY92_000176"
     CDS             7255..8769
                     /locus_tag="PPY92_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HDJ2624208.1"
                     /translation="MTNFFDILSERKGQLFSTMIEHIQISFIALLIATAIAVPLGILL
                     TKTKTISEIVMNIAAILQTIPSLALLGLMIPLFGIGRVPAIIALVVYALLPILRNTYT
                     GIKEVDPSLIEAAKGIGMKPFRRLTKVELPIAMPVIMAGVRTAMVLIIGTATLAALIG
                     AGGLGDLILLGIDRNNASLILLGAIPAALLAIIFDLILRFMAKLSYKKLLMTLGVIVM
                     IIILAIAIPMFAQKGDKITLAGKLGSEPSIITNMYKILIEEETKNTVEVKDGMGKTAF
                     LFNALKSDDIDGYLEFTGTVLGELTKEPLKSKEEKKVYEQAKQSLEKKYQMTMLKPMK
                     YNNTYALAVKRDFAKQHNIRTIGDLNKVKDQLKPGFTLEFNDRPDGYKAVQKAYNLNL
                     DNIRTMEPKLRYQAINKGNINLIDAYSTDAELKQYDMVVLKDDKHVFPPYQGAPLFKE
                     SFLKKHPEIKKPLNKLENKISDEDMQMMNYKVTVKNEDPYTVAKDYLKAKGLIK"
     gene            9009..10067
                     /gene="hisC"
                     /locus_tag="PPY92_000177"
     CDS             9009..10067
                     /gene="hisC"
                     /locus_tag="PPY92_000177"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDJ2624209.1"
                     /translation="MKEQLNQLSAYQPGLSPRALKEKYGIEGDLYKLASNENLYGPSP
                     KVKEAISAHLDELYYYPETGSPTLKAAISKHLNVDQSRILFGAGLDEVILMISRAVLT
                     PGDTIVTSEATFGQYYHNAIVESANVIQVPLKDGGFDLEGILKEVNEDTSLVWLCNPN
                     NPTGTYFNHESLDSFLSQVPPHVPVIIDEAYFEFVTAEDYPDTLALQQKYDNAFLLRT
                     FSKAYGLAGLRVGYVVASEHAIEKWNIIRPPFNVTRISEYAAVAALEDQQYLKEVTHK
                     NSVERERFYQLPQSEYFLPSQTNFIFVKTKRVNELYEALLNVGCITRPFPTGVRITIG
                     FKEQNDKMLEVLSNFKYE"
     gene            10406..10948
                     /locus_tag="PPY92_000178"
     CDS             10406..10948
                     /locus_tag="PPY92_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'(3')-deoxyribonucleotidase"
                     /protein_id="HDJ2624210.1"
                     /translation="MTRKSIAIDMDEVLADTLGEIIDAVNFRADLGIKMEALNGQKLK
                     HVIPEHDGLITEVLREPGFFRHLKVMPHAQEVVKKLTEHYDVYIATAAMDVPTSFSDK
                     YEWLLEFFPFLDPQHFVFCGRKNIVKADYLIDDNPRQLEIFTGTPIMFTAVHNINDDR
                     FERVNSWKDVEQYFLDNIEK"
     gene            complement(11605..12522)
                     /locus_tag="PPY92_000179"
     CDS             complement(11605..12522)
                     /locus_tag="PPY92_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDJ2624211.1"
                     /translation="MENKYTHGVLFYHEHSGLKNINQGIGEVTTALSSICKHLSIQLS
                     ENEGDIIKYCQEIKTKNYAKDVDILFILGGDGTVNELINGVMSHDLQLPIGILPGGTF
                     NDFTKTLNIAPNHKQASEQMISAQVGTYDVIKINNQYALNFVGLGLIVQNAENVQDGS
                     KDIFGKLSYIGSTVKTLLNPTQFNYQLSIDDKTYSGETTMILTANGPFIGGSRIPLTD
                     LSPQDGELNTFIFNEQSFSILNDIFKKRDSMNWNEITQGIEHIPGKKISLTTDPAMKV
                     DIDGEISLETPIDIEVIPNAIQLLTVNDL"
     gene            12613..12714
                     /locus_tag="PPY92_000180"
     CDS             12613..12714
                     /locus_tag="PPY92_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624212.1"
                     /translation="MVITDIDVYRVGRENIVIAVYNEHIKLLFILIS"
     gene            complement(12792..14297)
                     /locus_tag="PPY92_000181"
     CDS             complement(12792..14297)
                     /locus_tag="PPY92_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDJ2624213.1"
                     /translation="MTQQNSHGNQIQDIPQTGFFGHPRGLGVLFFVEFWERFSYYGMR
                     ALLIFYMYFAVTDNGLGIDKTTAMSIMSVYGSLIYMTSIPGGWIADRITGTRGATLLG
                     AVFIIIGHICLSLPFALIGLFTSMFFIIIGSGLMKPNISNIVGRLYPENDRRMDAGFV
                     IFYMSVNMGALLSPIILQHFVNVKNFHGGFLIAAVGMALGLVWYVLFNRKNIGSVGMK
                     PTNPLTPAEKKKYGLIIGSVVLAIVLIIVIGALTNSLSFNLVSNTVLVLGIALPIIYF
                     TLIIRSKDVTDTERSRVKAFIPLFILGMVFWAIQEQGSNVLNIYGIEHSDMKLNLFGW
                     KTNFGEAIFQSINPLFILLLAPIISLLWQKLGTKQPSLPVKFAIGTFLAGASYILIGI
                     VGYASGSSNFSVNWVILSYIICVIGELCLSPTGNSAAVKLAPKAFNAQMMSIWYLTNA
                     SAQAINGTLVKLIEPLGQTNYFIFLGVVAIIVTTIVLAFSPLIIKAMKGIR"
     gene            complement(14638..15138)
                     /gene="queF"
                     /locus_tag="PPY92_000182"
     CDS             complement(14638..15138)
                     /gene="queF"
                     /locus_tag="PPY92_000182"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDJ2624214.1"
                     /translation="MAHGRQQDELQDITLLGNQDNTYNFDYRPDVLESFDNKHQGRDY
                     FVKFNCPEFTSLCPITGQPDFATIYISYIPNVKMVESKSLKLYLFSFRNHGDFHEDCM
                     NIIMNDLIELMDPHYIEVWGKFTPRGGISIDPYTNYGRPNSKYEKMAEHRLMNHDLYP
                     EKIDNR"
     gene            complement(15158..16024)
                     /locus_tag="PPY92_000183"
     CDS             complement(15158..16024)
                     /locus_tag="PPY92_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDJ2624215.1"
                     /translation="MNPKVKGIIAILISAIGFSFMSVFFRLAGDLPVFQKSLARNLVA
                     MFIPLFFLYKYHQPMFGRLSSQPLLITRSTLGLIGVLLNIYAIDHMVLSDADSLMKLN
                     PFWTILLSIVFLHEKVRKYQITAMIIAILGMLLIVKPEFSSSMIPSLAGLFSGIFAAS
                     AYTCVRALSSREAPYTIVFYFSLFSVIVLIPFTAYTYEPMSQMQILYLLGAGLAAAVG
                     QIGVTLAYSFAAAKDISIFTYASIIFTAILGFVLFGESPDFYATLGYVVIIGASYYMF
                     EKARRDAKIIKK"
     gene            16825..17223
                     /gene="nrdI"
                     /locus_tag="PPY92_000184"
     CDS             16825..17223
                     /gene="nrdI"
                     /locus_tag="PPY92_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDJ2624216.1"
                     /translation="MKIIYFSFTGNVRRFIKRTELENTLEITAENCMEPVHEPFIIVT
                     GTIGFGEVPEPVQSFLEVNHQYIRGVAASGNRNWGLNFAKAGRTISEEYNVPLLMKFE
                     LHGKNKDVIEFKNKVGNFNENHGREKVQSY"
     gene            17186..19291
                     /gene="nrdE"
                     /locus_tag="PPY92_000185"
     CDS             17186..19291
                     /gene="nrdE"
                     /locus_tag="PPY92_000185"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDJ2624217.1"
                     /translation="MKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEVK
                     DKTIFFDTEIERLRYLVDNDFYFNVFDIYSEADLIEITDYAKSISFNFASYMSASKFF
                     KDYALKTNDKSQYLEDYNQHVAIVALYLANGNKAQAKQFISAMVEQRYQPATPTFLNA
                     GRARRGELVSCFLLEVDDSLNSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIK
                     GVAKGVLPIAKSLEGGFSYADQLGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRL
                     STISTGLIVPSKFFDLAKEGKDFYMFAPHTVKEEYGVTLDDIDLEKYYDDMVANPNVE
                     KKKKNAREMLNLIAQTQLQSGYPYLMFKDNANRVHPNSNIGQIKMSNLCTEIFQLQET
                     SIINDYGIEDEIKRDISCNLGSLNIVNVMESGKFRDSVHSGMDALTVVSDVANIQNAP
                     GVRKANSELHSVGLGVMNLHGYLAKNKIGYESEEAKDFANIFFMMMNFYSIERSMEIA
                     KERGIKYQDFEKSDYANGKHFEFYTTQEFEPQFEKVRELFDGMAIPTSEDWKKLQQDV
                     EQYGLYHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPQTM
                     WYYKSAFNTDQMKLIDLIATIQTHIDQGISTILYVNSEISTRELARLYVYAHYKGLKS
                     LYYTRNKLLSVEECTSCSI"
     gene            19409..20380
                     /gene="nrdF"
                     /locus_tag="PPY92_000186"
     CDS             19409..20380
                     /gene="nrdF"
                     /locus_tag="PPY92_000186"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDJ2624218.1"
                     /translation="MIAVNWNTQEDMTNMFWRQNISQMWVETEFKVSKDIASWKTLSE
                     AEQDTFKKALAGLTGLDTHQADDGMPLVMLHTTDLRKKAVYSFMAMMEQIHAKSYSHI
                     FTTLLPSSETNYLLDEWVLEEPHLKYKSDKIVANYHKLWGKEASIYDQYMARVTSVFL
                     ETFLFFSGFYYPLYLAGQGKMTTSGEIIRKILLDESIHGVFTGLDAQHLRNELSESEK
                     QKADQEMYKLLNDLYLNEESYTKMLYDDLGITEDVLNYVKYNGNKALSNLGFEPYFEE
                     REFNPIIENALDTTTKNHDFFSVKGDGYVLALNVEALQDDDFVFDNK"
     gene            20561..20716
                     /locus_tag="PPY92_000187"
     CDS             20561..20716
                     /locus_tag="PPY92_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624219.1"
                     /translation="MFYFTLSKQNTHSEKLNFSLIANINKIDTKLFERLLKLWSKNAT
                     INEKYYQ"
     gene            20755..21726
                     /locus_tag="PPY92_000188"
     CDS             20755..21726
                     /locus_tag="PPY92_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDJ2624220.1"
                     /translation="MKYLLKGNILLLLLILLTIISLFIGVSELSIKDLLHLTESQRNI
                     LFSSRIPRTMSILIAGSSLALAGLIMQQMMQNKFVSPTTAGTMEWAKLGILIALLFFP
                     TGHILLKLVFAVICSICGTFLFVKIIDFIKVKDVIFVPLLGIMMGGIVASFTTFISLR
                     TNAVQSIGNWLNGNFAIITSGRYEILYLSIPLLALTYLFANHFTIVGMGKDFTNNLGL
                     SYEKLINIALFITATITALVVVTVGTLPFLGLVIPNIISIYRGDHLKNAIPHTMMLGA
                     IFVLFSDIVGRIVVYPYEINIGLTIGVFGTIIFLILLMKGRKNYAQQ"
     gene            21713..22669
                     /locus_tag="PPY92_000189"
     CDS             21713..22669
                     /locus_tag="PPY92_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDJ2624221.1"
                     /translation="MRNNNKKIMLLIAVTLLISMLYLFVGIDFEIFEYQFSSRLRKFI
                     LIILVGAAIATSVVIFQAITNNRLLTPSIMGLDAVYLFIKVLPVFLFGIQSVWVTNVY
                     LNFILTLITMVLFALILFQGIFKIGHFSIYFILLIGVLLGTFFRSITGFIQLIMDPES
                     FLAIQSSMFANFNASNSNLVTFSAVLLVILLVITILLLPYLDVLLLGRAEAINLGISY
                     EKLTRILLVIVSVLVSVSTALVGPITFLGLLTVNLAHELMKTYEHKYILIATICLSWI
                     SLFSAQWVVENVFEATTEMSILIDLIGGSYFIYLLVRRRNAQ"
     gene            22666..23427
                     /locus_tag="PPY92_000190"
     CDS             22666..23427
                     /locus_tag="PPY92_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDJ2624222.1"
                     /translation="MIQVENLTKTINNQMILEDISIDIEKGKLTSLIGPNGAGKSTLL
                     SAICRLIRFDNGEVKIDGQLMSDYKNNDLSKKISILKQTNHTEMNITVEQLVNFGRFP
                     YSKGRLTKEDHDIVNDALDLLQLQDIRNRNIKSLSGGQRQRAYIAMTIAQDTEYILLD
                     EPLNNLDMKHAVQIMQTLKMLAHKMNKAIVIVLHDINFASCYSDQIVALKNGQLVKSD
                     LKDNVIQSSVLSDLYDMNIQIEHIRNQRICLYFKD"
     gene            23546..24574
                     /locus_tag="PPY92_000191"
     CDS             23546..24574
                     /locus_tag="PPY92_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDJ2624223.1"
                     /translation="MKKTVLYLVLAVMFLLAACGNNSDKEQSKSETKGSKDTVKIENN
                     YKMRGEKKDGSDAKKVKETVEVPKNPKNAVVLDYGALDVMKEMGLSDKVKALPKGEGG
                     KSLPNFLESFKDDKYTNVGNLKEVNFDKIAATKPEVIFISGRTANQKNLDEFKKAAPK
                     AKIVYVGADEKNLIGSMKQNTENIGKIYDKEDKAKELNKDLDNKIASMKDKTKNFNKT
                     VMYLLVNEGELSTFGPKGRFGGLVYDTLGFNAVDKKVSNSNHGQNVSNEYVNKENPDV
                     ILAMDRGQAISGKSTAKQALNNPVLKNVKAIKEDKVYNLDPKLWFFAAGSTTTTIKQI
                     EELDKVVK"
     gene            complement(24891..25205)
                     /locus_tag="PPY92_000192"
     CDS             complement(24891..25205)
                     /locus_tag="PPY92_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624224.1"
                     /translation="MPKVYGSLIDTETRCRHYFTEEDIIAIKFKCCNKYYPCYKCHNE
                     FEKHAIKRWSEPSFNEKAILCGVCKHELTINEYMMVERCPNCQSRFNNRCKYHYHIYF
                     EI"
     gene            complement(25223..26146)
                     /locus_tag="PPY92_000193"
     CDS             complement(25223..26146)
                     /locus_tag="PPY92_000193"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDJ2624225.1"
                     /translation="MINKDIYQALQQLIPNEKIKVDEPLKRYTYTKTGGNADFYITPT
                     KNEEVQAVVKYAYQNEIPVTYLGNGSNIIIREGGIRGIVISLLSLDHIDVSDDAIIAG
                     SGAAIIDVSRVARDYALTGLEFACGIPGSIGGAVYMNAGAYGGEVKDCIDYALCVNEQ
                     GSLIKLTTKELELDYRNSIIQKEHLVVLEAAFTLAPGKMTEIQAKMDDLTERRESKQP
                     LEYPSCGSVFQRPPGHFAGKLIQDSNLQGHRIGGVEVSTKHAGFMVNVDNGTATDYEN
                     LIHYVQKTVKEKFGIELNREVRIIGEHPKES"
     gene            complement(26273..26791)
                     /locus_tag="PPY92_000194"
     CDS             complement(26273..26791)
                     /locus_tag="PPY92_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDJ2624226.1"
                     /translation="MYSNVQPFINSDLTNCFQSQYTEIESLLFNLLDSPVKYTKHIGG
                     THHFKYSTEPILDILVGVENLHDITSLDEKRLNYVGFYRLHHKYTKKVMMAKFNNFHE
                     LKQQVRLHIIQMNTQTFESYLALDYLLSTNDDIALQFNTKKHQILQHSLTIRQYEMQK
                     QQYFEKLCKKYL"
     gene            26911..27789
                     /locus_tag="PPY92_000195"
     CDS             26911..27789
                     /locus_tag="PPY92_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EMYY motif lipoprotein"
                     /protein_id="HDJ2624227.1"
                     /translation="MKKIVIIAVLAILFVVISACGNKEKEAQHQFTKQFKDVEQKQKE
                     LQHVMDNIHLKEIDHLSKTDTTDKNSKEFKALQEDVKNHLIPKFEAYYKSAKNLPDDT
                     MKVKKLKKEYMTLANEKKDAIYQLKKFIGLCNQSIKYNEDILDYTKQFEKNRYKVESE
                     IKLADNKSEATNLTTKLEHNNKALRDTAKKNLDDSKENEVKGAIKNHIMPMIEKQITD
                     INQTNISDKHVNNARKNAIEMYYSLQNYYNTRIETIKVSEKLSKVDVDKLPKKGIDIT
                     HGDKAFEKKLEKLEEK"
     gene            27943..28263
                     /gene="ytxJ"
                     /locus_tag="PPY92_000196"
     CDS             27943..28263
                     /gene="ytxJ"
                     /locus_tag="PPY92_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HDJ2624228.1"
                     /translation="MVIKLSSIDQFEQVIEENKYVFVLKHSETCPISANAYDQFNKFL
                     YERDMDGYYLIVQQERDLSDYIAKKTNVKHESPQAFYFVNGEMVWNRDHGDINVSSLA
                     QAEE"
     gene            28687..29811
                     /locus_tag="PPY92_000197"
     CDS             28687..29811
                     /locus_tag="PPY92_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDJ2624229.1"
                     /translation="MKVLVAMDEFHGIISSYQANRYVEEAVASQIETADVVQVPLFNG
                     RHELLDSVFLWQSGQKYRIPVHDADMNEVEGVYGQTDTGMTVIEGNLFLKGKKPIVER
                     TSYGLGEMIKHALDNDAKHVVISLGGIDSFDAGAGMLQALGAQFYDDEGRVVDMRQGA
                     GVIKYIRRMDMSNLHPKMETARIQVMSDFSSRLYGKQSEIMQTYDAHQLHHNQAAEID
                     NLIWYFSELFKSELKIAIGPVERGGAGGGIAAVLNGLYQAEILTSHALVDQLTHLENL
                     VEQADLIIFGEGLNENDQLLETTTLRIAELCHKHQKVAIAICATAEKFDLFESQGVTA
                     MFNTFIDMPETYTDFKMGLQIRHYTVQSLKLLKTHFNVEV"
     gene            complement(29998..31224)
                     /gene="pepT"
                     /locus_tag="PPY92_000198"
     CDS             complement(29998..31224)
                     /gene="pepT"
                     /locus_tag="PPY92_000198"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDJ2624230.1"
                     /translation="MKNQLIDRLTRYTTIDTQSDPKSTTTPSTEKQWDLLHLLEKELQ
                     QLGLPTDLDENGYLFATLESNIDADVPTVGFLAHVDTSPDFNASNVKPQIIENYDGKP
                     YKLGNTKRVLDPKVFPELNSLVGHTLMVTDGTSLLGADDKAGIVEIMEAICYLQEHPE
                     IKHGTIRIGFTPDEEIGRGPHKFDVDRFNADFAYTMDGSQYGELQYESFNAAEAVITC
                     HGVNVHPGSAKNAMVNAIRLGEQFDSLLPDSEVPERTEGYEGFYHLMNFEGTVEKATL
                     QYIIRDHDKKQFELRKKRILEIRDDINAHFENYPVKVDISDQYFNMAEKILPLPHIID
                     IPKRVFAKLDIPANTEPIRGGTDGSQLSFMGLPTPNIFTGCGNFHGPYEYASIDVMEK
                     AVQVIIGIVEDIAENH"
     gene            complement(31238..31732)
                     /locus_tag="PPY92_000199"
     CDS             complement(31238..31732)
                     /locus_tag="PPY92_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDJ2624231.1"
                     /translation="MLFYLFHFTISFISTVLFSIIFNAPKRLLVACGFVGAIAWTIYQ
                     LTVDLEFGKVGASFLGSLILGLMSHTMSRRYKRPVIIFIVPGIIPLVPGGAAYQATRF
                     LVSNDYTSAVNTFLEVTLISGAIAFGILVSEILYYLYTRIKQLYGKIKGKTYKKSYNM
                     NNRV"
     gene            complement(31750..32511)
                     /locus_tag="PPY92_000200"
     CDS             complement(31750..32511)
                     /locus_tag="PPY92_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDJ2624232.1"
                     /translation="MPDSITIIDENKVIDVVLIAGRILLESGAETYRVEDTMNRIAHS
                     YGLHNTYSFVSSTAIIFSLNDRTSTRLIRVQERTTDLEKIALTNSLSRKISNKELTID
                     EAKSEFIHLQHASLQYSFLTNFFAAAIACGFFLFMFGGVASDCWIAVIAGGSAFLTFS
                     FVQRYIQIKFFSEFVAAAVVISIAATFTKLGIATNQDIITIASVMPLVPGILITNAIR
                     DLLAGELLAGMSRGVEAALTAFAIGAGVAIVLLII"
     gene            complement(32707..33777)
                     /locus_tag="PPY92_000201"
     CDS             complement(32707..33777)
                     /locus_tag="PPY92_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDJ2624233.1"
                     /translation="MFEAFIYNISVIVAGIYLFHRLQYSENKRMVFSKAYVTVLMTIV
                     SLLLSVYPIPYREDYLIHLTFVPLLFLGRFTNMVYTLSATVIVAIVEIVVFNNSIMYG
                     VTLIVIAAVTSAIGPFLKQNDVLSLLILNVVTIIILFGVALVSPIYTLSEVIILIPIS
                     LIITLASAITFVDIWHFFSLVNRYENEDKYDYLTGLGNVKEFDRHLNEISRKAEKEHQ
                     SIALLLIDIDGFKDVNDTYSHKSGDAVLKQMSQLLKNYVPNQFKIFRNGGEEFSVVIH
                     NYSLDQSVKLAENIRSGVEKSSFHLPNKEVIKLSVSIGVGYLTDDDPKSQRKVFKDAD
                     DMVHVAKNQGRNKVMFNPIINL"
BASE COUNT        12248 a         4871 c         5911 g        10971 t
ORIGIN      
        1 atcaaagttg aaagctacaa gtaatgaaaa ttataaactt gaatctgaaa gtaattacta
       61 taattatgac aatgttaact tttaaacgca cttattaatt aactacataa tgttaatatc
      121 taatttattc aagtactttc gcaagattta ttatctaaat aacgggggaa agaatcatga
      181 gttcacaaaa aaagaaaatt agtctttttg cgttcttctt attaaccgta ataacgatta
      241 ccttgaagac gtatttttct tattatgttg atttttcttt aggtgttaaa ggtttagtac
      301 aaaacttaat attattgatg aatccttata gtttagtagc actggtttta agtgtgttcc
      361 tattctttaa aggcaaaaaa gcattttggt tcatgttcat aggcggcttc ttattgacgt
      421 tcctattata tgccaatgtt gtgtacttta gattcttctc tgatttttta acgtttagta
      481 ctttaaacca agtaggtaac gtagaatcta tgggtggtgc ggttagtgca tcattcaaat
      541 ggtatgactt tgtttatttc attgatacgt tagtttactt attcatttta atatttaaaa
      601 caaaatggtt agacacaaaa gcatttagta agaaatttgt tcctgtcgta atggcagctt
      661 cagtagcatt attcttctta aacttagctt ttgctgaaac tgacagacca gaattattaa
      721 cacgtacatt tgaccataaa tatttagtga aatatttagg accttataac tttacagtat
      781 acgatggtgt taaaactatc gaaaataatc aacaaaaagc gttagcatct gaagatgact
      841 taacaaaagt attaaattat acgaaacaac gtcaaacaga gcctaaccca gaatattatg
      901 gggtggcaaa gaagaaaaat attattaaga ttcatttaga aagtttccaa accttcttaa
      961 ttaataaaaa ggttaatggt aaagaagtaa caccgttttt aaacaaatta tcaagtggga
     1021 aagagcaatt cacatacttc cctaactttt tccatcaaac aggtcaaggt aaaacatctg
     1081 actctgaatt tacaatggat aacagtttat acggtttacc gcaaggttct gccttttcat
     1141 taaaaggaga taatacgtat cagtcattac cagcaatttt agatcaaaag caaggctaca
     1201 aatctgatgt catgcacggt gactataaaa cattctggaa cagagaccaa gtatataaac
     1261 actttggtat cgataaattc tatgatgcaa catactatga catgtcagat aaaaacgttg
     1321 taaacttagg cttgaaagac aaaattttct ttaaagattc tgctaattat caagctaaga
     1381 tgaaatcacc attctattct catttaatta cattgactaa ccactatcca ttcacattag
     1441 atgaaaagga tgcgactatt gagaagtcaa acacaggtga tgcaacagtt gatggttata
     1501 ttcaaacagc gcgttattta gacgaagcat tagaagaata tattaatgac ttgaagaaaa
     1561 aaggattata tgacaattca gtgattatga tttatggtga ccactatggt atctctgaaa
     1621 accataacaa tgccatggaa aaactattag gtgaaaaaat cacaccagct aaatttacag
     1681 atttaaacag aactggtttc tggattaaaa tccctggtaa atctggtggt atcaataatg
     1741 aatatgctgg tcaagtcgat gtaatgccaa caattttaca tttggctggt atagatacga
     1801 agaattattt aatgttcggt actgatttat tctctaaagg tcataatcaa gtagttccat
     1861 tcagaaatgg tgactttata acaaaagatt ataaatatgt taatggtaag atttattcta
     1921 ataaaaataa tgaactcata actactcaac cagctgattt cgaaaagaat aaaaagcaag
     1981 ttgaaaagga tctcgaaatg agtgacaacg tgcttaatgg tgatttgttt agattctaca
     2041 aaaatccaga cttcaaaaag gtaaatcctt cgaagtataa atatgaaaca ggacctaaag
     2101 caaactctaa aaaataatat ttaaacacga actcggattg ataaaatatc aatccgggtt
     2161 ttattatgtt cttttatatt atgttttaca gtatatgtgt tgataaaaag gtatattata
     2221 aaaatcatta gcatagtcat caaagcctta tcaagtttta tttatagttg aagcaacaat
     2281 atattaaatt atgacactta acggtatcga tttcttttga tttcaaaaag gtgtataatg
     2341 tatagaactg atttaagtac gtagagattt caacagaaga aggaagatgg gtatggaagc
     2401 atataaaatt gaacatttaa ataaatctta tgccgataag actatattcg ataacctaga
     2461 tttatcaatt tcagaaggtg aaaaaatagg tttggtgggc ataaatggta cagggaaaag
     2521 tacgttgtta aaagtaattg gtggtattga tgatgatttt acagccaatg ttatgcatcc
     2581 aaatcaatat cgaattcgat attcgtctca gaaacaggac cttaatgaag atatgacagt
     2641 ttttgatgca gtattaagtt ctgatacaac aactttacgc atcatcaagc aatatgagca
     2701 ggcagtacaa gcttatgcgg atgaccaaag tgataaattg ttcaagcgaa tgatagatgc
     2761 gcaagatgct atggatcaac atgatgcttg ggactataac gctgaaatta aaacaatcct
     2821 ctcaaaacta ggtatacatg atactactaa atacattaaa gaattatccg gcggacaaca
     2881 aaaacgtgtt gtacttgcta aaacattaat agaacaacca gatttattgt tattagatga
     2941 acctacgaac catttagact tcgaatcaat cagctggttg atcaattatg tgaagcaata
     3001 tcctcataca gttttattcg taacccatga tcgatatttt ttaaatgaag tttccactag
     3061 aattattgaa ctaaacagag gtaagttagc gtcatatcct ggtaactatg aatcttatat
     3121 tgaaatgcgc gctgaaagag aagtaacact tcaaaagcaa caacaaaagc aacgagcttt
     3181 atataaggaa gaacttgctt ggatgagggc tggagctaag gctcgtacta caaagcaaca
     3241 agctagaatt aatcgattta atgacctaga aaatgaagtt aaccagcaat ataaagacga
     3301 taaaggtgaa ttgaatcttg cttattcaag attaggtaag caagtgttcg aattagaaga
     3361 cttatcaaag gctattaatg ataaagtatt atttgaacat ctgacggaaa ttattcaaaa
     3421 aggtgagcgt attggtgttg ttgggccaaa tggagctggt aaaacaacac tcttaaatat
     3481 tttgagtgga gaagaccaac aattcgaagg taaattgaag actgggcaga cggttaaagt
     3541 agcttatttt aagcaaacag atgagaccct ggatagagat attcgtatga ttgattattt
     3601 aagagaagaa agtgaggtcg caaaagaaaa agatggaacc tcggtatcta ttacacaact
     3661 tcttgaacga tttttatttc caagtgcaac tcatggtaaa aaagtttata aattatctgg
     3721 tggagagcaa aagcgtttgt atttattacg tctactcgta caccagccaa atgttctgtt
     3781 gttagatgaa ccgacaaatg atttagatac tgagacttta acaatacttg aagattatat
     3841 tcatactttc ggtggtacag tgattaccgt aagccatgat cgctacttct taaataaagt
     3901 tgcacagtca tattggttta ttcatgatgg tcagatggaa aagattatcg gaacttttga
     3961 agattatgaa agttataaaa aatcattaga taaaaataaa tccacattga agcaacaatc
     4021 taaatcttat acaactgtac gtaagaaaaa tggtttatca tataaagaaa aattagaata
     4081 tgaacaattg atgaaacgca tagaacaagc ggaagtaaga atggaagaaa ttgatgtgct
     4141 catgattgag gcaagtgcag attatgggaa aattaaagaa ctaaacgaag aaaaagaaca
     4201 acttgaaatt caatatgatt tagacatcac aagatggagt gagttagaag aaattaaaga
     4261 acaacaataa ggggtcaatt tatgatgcaa caaacattat cgcattactt tgggtatgaa
     4321 acatttcgac caggacaaga agaaattatt agcaaagtat tagaccatcg taatgtgctt
     4381 ggtgtcttac caactggtgg aggtaagtct atatgctatc aagtaccagg tttattgtta
     4441 ggtggtacaa caattgtaat aagtccacta atatcattaa tgaaagatca agtggatcaa
     4501 ttaaaagcga tgggaattca agctgctttt ttaaatagta gtttgactca aaaagagcaa
     4561 caacgtattg aaaaagcatt atcaaatgga gaaattcaat ttttgtatgt tgcaccagaa
     4621 cgatttgaaa accgatattt tttaaatatg cttcagcgta taaagattca cttagtcgcg
     4681 tttgatgaag cgcattgtat ttctaaatgg ggtcatgatt tcaggccgag ttaccaaaat
     4741 gttatttcaa aagtatttac gttacctcaa gattttacaa taatagcgtt gacagcaact
     4801 gccacggttg aagtacagca agatattaga gaaaagttaa atatcgctca aactgatcaa
     4861 attaaaacga gtactaagcg tagaaactta atttttaaag taaatcctac ttatcaacgt
     4921 caaaaattta tattggatta tattaaaaca catgatgaag atgcaggtat tatttattgt
     4981 tctacacgta agcaagttga agagcttcaa gaagccttag aaagtcagaa aattgaaagt
     5041 gttatatatc atgcaggttt gagcaataaa gaaagagaag aagcgcagaa tgatttctta
     5101 tttgatcgtg ttaaagtagt cgttgctaca aatgcttttg gtatgggtat tgataaatcc
     5161 aatgtacgtt ttgttattca ttataatatg cctggagatt tagaatctta ttatcaagaa
     5221 gcgggtcgtg caggtcgtga cgggttgaaa agtgaatgta ttttgttatt tagcgaacgc
     5281 gatatcaatt tacacgagta ttttataaca gtctctcaag ctgatgatga ctataaagat
     5341 aaaatgggcg aaaagttaac taaaatgatt caatatacaa aaacaaaaaa atgtctagaa
     5401 gcaacaattg tccattattt tgaaccgaat gaaaaattag aagaatgtga acaatgtagt
     5461 aattgtgttc aacaagataa atcatataat atgacacaag aagctaagat gattattagt
     5521 tgcatcgctc gtatgaaaca acaagagagt tatagtgtta tcattcaagt gttaagagga
     5581 gagtcaacag attatattaa gtataaaggt tatgaccaaa tttcaaccca tggtttaatg
     5641 aaaggttaca caacatcaga attaagtcac ttaatagatg aattaagatt caaagggttc
     5701 ttaaatgaaa atgacgaaat attaatgtgt gatacttcaa ttaaaaaatt actcagtaat
     5761 gaagtagaag tattcacaac accatttaag caaaaagcga ctgaaaaagt atttataaat
     5821 acggttgaag gggttgaccg agtattattc agtcagttgg tagaagttcg taaaaagtta
     5881 agtgacaaat taacgatagc acctgtaagt atattttctg attacacgtt ggaggaattt
     5941 gctaaacgta agcctgcttc gaaacaagat atgattaata ttgatggcgt aggtagttac
     6001 aaattaaaac attattgtcc agcattttta gaaacgattc aaaattataa agccaaagta
     6061 tagtgaaggc acctagtaag cattccttgt tttactgggt gtttttttga attcaaggta
     6121 aaataacact atttcttaac tggagattac ctattattca ttattttgtt aaaaaagttc
     6181 aaggataaat catttaaatt tgaaaattgt aataaacgtg gttaaatagg gaatacatac
     6241 atataatctc ggttttatgc tatgagaaaa aaagaggtgg caaagtgatt aagtttaaaa
     6301 atgtaactaa gcgttatggc aaacatgttg ctgtcgataa cattagtttc aatattaatg
     6361 agggtgaatt ttttgtgcta attggacctt caggttgtgg aaaaactacg acattaaaaa
     6421 tgattaatcg actcattcac ttaagtgaag gttatattta ttttaaagat aaaccaataa
     6481 gtgattatcc agtatacgaa atgcgttggg atattggata cgtattgcag cagattgcat
     6541 tattcccaca tatgacgatc aaagaaaata ttgcacaagt gccacaaatg aaaaagtgga
     6601 aagaaaaaga tatagataaa agagtagatg aattacttga tatggttgga ttagaacctg
     6661 aaaaatataa aaacagaaaa cctgatgaat tgtcaggggg gcaacgacaa cgtgtaggag
     6721 ttatacgtgc gttagcagct gatccaccag ttattttaat ggatgaaccg tttagtgcat
     6781 tagacccaat cagccgagaa aaacttcaag atgatttaat tgaattacaa actaaaatta
     6841 agaagacaat catatttgtt acacatgata ttcaagaggc gatgaaactt ggtgataaga
     6901 tttgtctttt gaatgaaggg catattgaac aaattgacac accagaagga tttaaaaata
     6961 atcctcaaag tgaatttgtt aaacaattta tgggtagtca tttagaagat gatgcgccat
     7021 gtgttgaaga gaacgcaatt attcgtgact tggatattat gaaaccaatc gatgaggtta
     7081 catctatgag cgcttatcca attgtttatg acaatcaacc aattgaagta ttgtatcaac
     7141 atttatcaga gagcgagcgt gtcattgtca tgcaagaaga tagcgtaggt caatatgtta
     7201 ttgataggaa agatatcttc aaatatttgt cccagaaaaa ggaggtagct caacatgact
     7261 aactttttcg acatattgag tgaacgtaag gggcaactct tttcgacaat gatagaacat
     7321 attcaaatat catttattgc attattgatt gcaactgcta ttgcggtacc attaggtatt
     7381 ttattaacga agactaaaac gatatctgaa atcgtaatga atattgcggc aattcttcaa
     7441 accataccat cgttggcatt attaggttta atgattcctt tatttggtat cggtcgtgtg
     7501 ccagcaatta ttgcacttgt agtgtatgcg ttgttaccaa ttttaaggaa tacgtatact
     7561 ggaattaaag aagttgatcc atcactcatt gaagcggcta aaggtatagg tatgaaacca
     7621 tttagacgtt taactaaagt cgaacttccg atagcaatgc ctgttataat ggctggtgta
     7681 agaacggcta tggtattaat tataggtaca gcgacactag cagcattaat tggtgcaggc
     7741 ggactaggag atttaatttt attaggtata gaccgtaaca atgcgtcatt gatattatta
     7801 ggtgcaattc cagcagcctt attggcaatt atatttgatt taattttaag atttatggct
     7861 aaattatctt ataaaaagtt attgatgacg ttaggtgtta tagtgatgat tattatactg
     7921 gccatcgcta ttcctatgtt tgcacaaaaa ggtgataaaa ttacgttagc tggaaagctt
     7981 ggctccgagc cttcgattat tacaaatatg tataaaattt taatagaaga agagaccaaa
     8041 aatactgtag aagtgaaaga tggtatgggg aaaacagcat ttttatttaa tgctttaaaa
     8101 tctgatgata tagatgggta tttagagttt actggaacag ttttaggtga attaacaaaa
     8161 gaaccattga agtcaaaaga agagaaaaaa gtttatgaac aagctaagca aagtcttgaa
     8221 aagaaatatc aaatgactat gttaaaacca atgaagtata acaatacgta tgctttagct
     8281 gtaaaacgtg attttgctaa acaacataat atacgtacaa ttggtgattt aaataaggtt
     8341 aaagatcaac ttaaaccagg atttacattg gaatttaatg atcgtccaga tggttacaaa
     8401 gctgttcaaa aggcttataa tttaaattta gataacatac gtacaatgga acctaagttg
     8461 agatatcaag cgatcaataa aggtaatatt aatttaatag atgcatattc aactgacgct
     8521 gaattaaaac aatatgatat ggttgtgtta aaagatgata agcacgtatt tccaccatat
     8581 caaggagcac cattatttaa agaaagcttt ttaaagaaac atccagaaat taagaaaccg
     8641 ttaaacaaac tagaaaacaa aatatctgat gaagatatgc aaatgatgaa ctataaagta
     8701 acagttaaaa atgaagaccc atatacagtt gcgaaagatt atttaaaagc aaaagggtta
     8761 atcaaataac gaccaacgcc acataagatg cgtaacacca aattatatct tatgtggcgt
     8821 tgttatattt aaatctataa ttatgttcaa tttaaacatg caataatgat taaaaaatat
     8881 gacatgttaa acacaatgta agctattatg atgtgaaaat agtagcattg cattttagaa
     8941 acatagagcg atataatgaa tataagtttt ttaaaatttc agttaattct aaggaggttg
     9001 tttttattat gaaagaacaa cttaatcaac tatcagcata tcagcctggt ttatctccaa
     9061 gggcattgaa agaaaagtat ggcattgaag gagatttata taaacttgca tcaaatgaaa
     9121 atttgtatgg accatcgcct aaagttaaag aagcgatatc agcacattta gatgagttat
     9181 attattatcc tgaaacagga tcaccgacat taaaagcggc gattagtaaa catttaaatg
     9241 tagatcaatc acgcatttta tttggtgcgg gattagatga agttatatta atgatttcta
     9301 gagcagtatt aacgccaggg gatactattg ttacaagtga agcgacattc ggtcaatatt
     9361 atcacaatgc gattgttgaa tcagctaatg tgatacaagt acctttaaaa gatggtggct
     9421 tcgatttaga aggtatttta aaagaagtta atgaagatac gtcattggta tggttatgta
     9481 atccaaataa tcctacaggt acatatttta atcatgagag cttagattcg tttttatctc
     9541 aagtacctcc acatgtacca gtaattatag atgaagctta ttttgaattt gtgacagcag
     9601 aggactaccc ggatacactt gctttgcaac aaaaatatga caatgctttc ttattacgta
     9661 cattttcaaa ggcgtatgga ttagcgggtt tacgtgtagg atatgtggta gcaagtgaac
     9721 atgcgattga aaaatggaac atcattagac caccatttaa tgtgacacgt atatctgaat
     9781 acgcagcagt tgcagcactt gaagatcaac aatatttaaa agaggtaaca cataaaaata
     9841 gtgttgaacg cgaaagattt tatcaattac ctcaaagtga gtatttcttg ccaagtcaaa
     9901 cgaattttat atttgtaaaa acaaagcggg taaatgaact ttatgaagca cttttaaatg
     9961 tagggtgtat tacgcgacca tttccaactg gtgttagaat tacaattggt tttaaagaac
    10021 aaaatgataa aatgttagaa gttttatcaa attttaaata cgaatagtaa gtggggagtg
    10081 ggacagaaat gatattttct caaaatttat ttcgtcgtcc caccccaact tgcattgcct
    10141 gtagaatttc ttttcgaaat tctctgtgtt ggggcccctg actagaattg aaaaaagctt
    10201 gttacaagcg cattttcgtt cagtcaacta ctgccaatat aactttgtag agcattgaac
    10261 attgatttat gtctcaagct caatgcagtg tgaatgatga ggtgagagta ttcagtgtaa
    10321 aaagcaacaa tagatgatat tgttttgtat caattgcttt tttgctatac tgaatcaata
    10381 ctgatatttt gaggagaaga ttaaaatgac ccgtaaatca atcgcgattg atatggatga
    10441 agtattggca gatacattag gagaaatcat tgatgctgtc aattttagag cggatttagg
    10501 tattaaaatg gaagctttga atggtcaaaa acttaaacat gttattcctg aacatgatgg
    10561 attaattaca gaagtattga gagaaccagg cttcttcaga catcttaaag tgatgccaca
    10621 tgcacaagaa gttgtgaaaa aattaactga acattatgat gtatatattg ctacagcagc
    10681 aatggatgta ccaacatcat ttagtgataa atatgaatgg ttactagagt tctttccatt
    10741 tttagatcct cagcattttg ttttttgtgg tagaaaaaac atcgttaaag ctgattattt
    10801 aatagatgac aatcctagac agcttgaaat ttttactggt acaccgatta tgtttacagc
    10861 agtgcataat attaatgatg atcgatttga acgcgtaaat agctggaaag atgtagaaca
    10921 gtatttttta gataatattg agaaataaaa tatatcactt gaaaaatttc atgtagaaaa
    10981 gatgatggat aggctataaa gtaattgtga ctgagatgaa cttttatgtc ttagacacta
    11041 caacactata ttggcagtag ttgactgcgg ggccccaaca tagagaaatt ggattcccaa
    11101 tttctacaga caatgcaagt tggcggggcc ccaacataga gaaattggaa cgccaatttc
    11161 tacagacaat gcaagttggg gtgggacgac gaaataaatt ttgcgaaaat atcatttctg
    11221 tcccactccc tcctattttt aagtacacat tagctttgac taatgataaa gaatcgctac
    11281 ataatcaatc attagtcgtt ctttatcatt tccgtcccgc tctcaataaa tgttagtcta
    11341 tctcattatt ataaatcgga tgaatgtgtt aatctatggc agattacacg tcatccgatt
    11401 ttttatagaa tttgaaaaag acgcataaac cactatgatt taaaatacaa catcaatcat
    11461 tttagtggca tgcgccaaaa ttatatgtct gtttttgaaa cagggtaata gcttaaagct
    11521 aataaaaacg aatataaggt gcgttgaatc ttatgattac actccaaacc taatataata
    11581 tcgggtcaag atcattccgg atgcttacaa atcattgaca gtaagtaact gaatagcatt
    11641 tggtataacc tcaatatcaa taggtgtttc taatgaaatt tcgccatcaa tatcaacttt
    11701 cattgctgga tctgttgtaa gtgaaatctt tttaccaggt atatgctcaa taccttgagt
    11761 aatttcattc caattcatgc tatcacgctt tttaaaaata tcatttaaaa tactgaaact
    11821 ttgttcatta aaaatgaaag tgttcagttc accatcttga ggagacaaat cagtcaatgg
    11881 tatacgacta ccaccaatga atggaccatt tgctgttagt atcatggtcg tttcgccaga
    11941 atatgtctta tcatctattg ataattgata attaaattgt gttggattta gcagtgtttt
    12001 gacagttgat ccaatataac tcaatttacc aaatatatct tttgaaccat cttgtacgtt
    12061 ttcagcgttt tgaacaatga gacctaagcc aacaaagttg agtgcatatt gattatttat
    12121 tttaattaca tcgtatgtac caacttgtgc agaaatcatt tgttcactag cttgtttatg
    12181 attaggtgct atatttagcg tttttgtaaa atcattaaaa gtaccgcctg gtaaaatgcc
    12241 aatagggagt tgaaggtcat gtgacataac accatttata agttcgttaa ccgtgccatc
    12301 accgccaaga ataaataata tatctacatc ttttgcatag tttttagttt tgatttcttg
    12361 gcaatattta ataatgtcac cttcgttttc actcaattga atagaaagat gcttacaaat
    12421 tgaacttaat gctgttgtaa cttccccaat accttgatta atatttttta atccactgtg
    12481 ttcatggtaa aagaggacac catgtgtata tttattttcc atagtttagc ctactttcta
    12541 aaaattggtt cattaaatat atatacccac ttttaattgt taataccaaa aatatgtttt
    12601 taaatagaga aaatggtaat aactgacatt gatgtctata gagtgggacg agaaaatata
    12661 gttatagctg tctataacga gcatattaag cttttattta tactgatatc ttgaatttaa
    12721 ttaatagaaa cctataaaaa aacagtaagc catttaaatg acttactgtt ttttgaatta
    12781 ggccaacaat attaacgtat acctttcatc gctttgatga ttaaaggtga gaatgctaat
    12841 acaattgttg taacaataat tgcaacaaca cctaggaaaa taaagtaatt tgtttgacct
    12901 agtggttcta ttaacttaac taaagtacca ttgattgctt gtgcagaagc gttagttaag
    12961 taccaaatac tcatcatttg ggcattaaat gctttaggtg ctaacttaac agcagcacta
    13021 ttacccgttg gtgataagca tagctcaccg ataacacaaa taatgtacga taaaataacc
    13081 cagttaactg agaagtttga tgaacctgat gcataaccta caataccaat tagtatgtat
    13141 gacgcacctg ctaagaacgt accaattgca aattttactg gcaggctagg ttgtttagtt
    13201 ccaagctttt gccataaaag tgaaataatt ggagctaata ataaaataaa taatgggtta
    13261 attgattgga agatcgcttc accaaagttt gttttccaac caaataagtt taatttcata
    13321 tctgaatgtt caattccata tatgtttaat acattagacc cttgttcttg aatagcccag
    13381 aacaccattc caagaataaa taatggaata aatgctttaa cacgagaacg ttcagtatca
    13441 gtgacatctt tacttctaat aattaaagtg aagtaaatga ttggtaatgc aatacctaat
    13501 actaaaacag tattactaac taagttaaat gataatgagt tagttaatgc accaataacg
    13561 ataattaata caattgctaa gacaacactt ccgataataa gaccatactt tttcttttca
    13621 gctggtgtca atgggttagt aggtttcata ccaacgctac ctatgttttt gcggttgaaa
    13681 agtacatacc atactaaacc taatgccata ccaactgctg caatcaagaa tccgccgtgg
    13741 aagtttttaa cattaacaaa gtgttgcaaa ataataggtg ataataatgc acccatatta
    13801 actgacatat agaaaataac aaaacctgca tccatacgtc tatcattttc aggatataaa
    13861 cggccaacga tatttgaaat gtttggcttc attaaacctg aaccaataat gatgaagaac
    13921 attgatgtga ataagccgat taatgcaaat ggtaagctta aacaaatatg tccgataata
    13981 ataaagactg cacctaataa agtagcgcct ctagtgcctg taattctgtc agcaatccat
    14041 ccgcctggta ttgatgtcat atagattaat gaaccataaa ctgacataat tgacatagct
    14101 gttgttttat caattccaag gccattatct gttacggcaa agtacatgta gaaaatgagt
    14161 agggcacgca tgccataata actaaacctt tcccagaact ctacaaagaa gagtacgcct
    14221 agtcctcgag gatgcccgaa aaatcctgtt tgaggtatgt cttgaatttg atttccatgg
    14281 gagttttgtt gtgtcatgta tacatcccat cctttcttcc cctaatacaa tagtttaagt
    14341 aaatttgatt gtgccttatc aactaaatat tccaacaaat cacaaaaaaa taaaaatatt
    14401 gagaatattc tgttattaag cgcgcacaat taattagaga taatataaaa atatacttat
    14461 tgacgttaaa gtgcaatagg tttctataaa aatgttgttt aattcgtgta ctaatattct
    14521 tttggtgtac tttggtataa aatgttattt taatgtaacc tattatgtat gctaaatgaa
    14581 aaacacacca ataagttagg aaaatcaact tattggtgtg tttattaaat gatacattta
    14641 acgattatct attttttcgg gatataaatc atgattcatc aaacgatgct cagccatttt
    14701 ttcatattta gaatttggac gtccatagtt tgtataagga tcaatagaaa ttccaccacg
    14761 tggtgtgaac ttgccccaga cttcaatata atgtgggtcc ataagctcta tcaaatcatt
    14821 cataataata ttcatacaat cttcgtgaaa atcaccgtga tttctgaaac taaataagta
    14881 taatttcaaa gattttgatt caaccatttt aacatttgga atatatgaaa tatagatagt
    14941 tgcaaaatct ggttgcccag taattggaca taatgatgta aattctggac agttgaattt
    15001 tacgaaatag tcacgacctt gatgcttatt atcaaacgat tctaatacat caggacgata
    15061 gtcaaaattg taagtattgt cttgatttcc taataaagtt atatcttgta attcatcttg
    15121 ttgacggcca tgtgccatat aaagcgctcc tttaaattta tttttttatt attttggcgt
    15181 ctcggcgtgc tttttcaaac atgtaataac ttgcaccgat aataacgacg taacctaatg
    15241 ttgcatagaa atctggagat tctccgaata gaacaaatcc aagtattgct gtgaaaatta
    15301 tagatgcata cgtaaaaata gaaatatctt ttgctgctgc aaaactatat gctaaagtaa
    15361 caccaatttg acccacagcg gcagctaagc cagcccctaa tagataaagt atttgcatct
    15421 gactcattgg ttcataagta tatgcagtga aaggtattaa aacgatgaca gaaaataagg
    15481 agaagtaaaa tactatagta tatggtgctt ctcttgaact aagtgctcga acacatgtat
    15541 atgctgatgc tgcaaaaata cctgagaata agccagctaa tgatggaatc atagatgatg
    15601 aaaattcagg tttcactatt aaaagcatac ctaaaatagc aattatcatt gctgtaattt
    15661 gatacttcct taccttttca tgtaagaaaa caatgcttaa taaaatcgtc cagaaaggat
    15721 tgagtttcat taatgaatcg gcatcactaa gtaccatatg atcaatggca taaatattta
    15781 acaatacacc aataagtcca agtgttgatc gtgttattaa taagggttga cttgaaagtc
    15841 tgccaaacat tggctgatgg tatttatata aaaaaaataa tggaataaac attgctacta
    15901 agtttcgtgc taatgatttt tgaaaaacag gaaggtcacc tgcaagtctg aaaaacactg
    15961 acataaaact gaaaccaata gccgaaatta aaatggcaat gatacctttt actttaggat
    16021 tcaattttat cgcctctttt atataaaatt aacgtattta tattagcata aaacaacatg
    16081 ttgtgcataa atagttgaaa tttactataa aaagactata atagactgta ccgaacaaac
    16141 gttctgtgtt tatttgtcgg aataataggg cattacactt ttatgaatgt ttgtgttatt
    16201 acataaaaca aatatcaatt cagtatcaag ctaataagct ttttcttgat ttctgttgat
    16261 acaattgaga ttgacacaga tttaaaaaaa tcaagtgata tctactaaaa aattttttta
    16321 aatttgttca agtttttcta atttagtatt ggtgcctagt tggaacgttt tacgaacatt
    16381 cgattagaaa atggcacttt aaatcatagt gtgtcttatg tataatgaaa cacataatat
    16441 agtgttggtg aaacgaaaaa gacacaatat cttgtgtttt gtatgcaaat gctttattta
    16501 tgaagaaatt acatttaaaa gtaatttaac acagaaattt aatagttatt atcaattaat
    16561 agtcatattt ttagaaaatg tactgagcaa atggaagata tccaatgatg taaatactac
    16621 atatagtgat ttttatacat tcaacccata taagctacta ttttctcaaa tataaatcta
    16681 tgcaattggt ttacatttga gaaaataagt agcttcatta tagttaatac aatgctgaga
    16741 taaccatagt aaccatgttg ttaaagcatt ttttaattgg aatgactact ttatttaaaa
    16801 gggttgaaga aagaaggtga tccaatgaaa ataatatatt tttcatttac tggaaatgtc
    16861 cgtcgtttta ttaagagaac agaacttgaa aatacgcttg agattacagc agaaaattgt
    16921 atggaaccag ttcatgaacc gtttattatc gttactggca ctattggatt tggagaagta
    16981 ccagaacccg ttcaatcttt tttagaagtt aatcatcaat acatcagagg tgtggcagct
    17041 agcggtaatc gaaattgggg actaaatttc gcaaaagcgg gtcgcacgat atcagaagag
    17101 tataatgtcc ctttattaat gaagtttgag ttacatggaa aaaacaaaga cgttattgaa
    17161 tttaagaaca aggtgggtaa ttttaatgaa aaccatggaa gagaaaaagt acaatcatat
    17221 tgaattaaat aatgaggtca ctaaacgaag agaagatgga ttctttagtt tagaaaaaga
    17281 ccaagaagct ttagtagctt atttagaaga agtaaaagac aaaacaatct tcttcgatac
    17341 tgaaatcgag cgtttacgtt atttagtaga caacgatttt tatttcaatg tgtttgatat
    17401 ttatagtgaa gcggatctaa ttgaaatcac tgattatgca aaatcaatct cgtttaattt
    17461 tgcaagttat atgtcagcta gtaaattttt caaagattac gctttgaaaa caaatgataa
    17521 aagtcaatac ttagaagact ataatcaaca cgttgccatt gttgctttat acctagcaaa
    17581 tggtaataaa gcacaagcta aacaatttat ttctgctatg gttgaacaaa gatatcaacc
    17641 agcgacacca acatttttaa acgcaggccg tgcgcgtcgt ggtgagctag tgtcatgttt
    17701 cttattagaa gtggatgaca gcttaaattc aattaacttt attgattcaa ctgcaaaaca
    17761 attaagtaaa attgggggcg gcgttgcaat taacttatct aaattgcgtg cacgtggtga
    17821 agcaattaaa ggaattaaag gcgtagcgaa aggcgtttta cctattgcta agtcacttga
    17881 aggtggcttt agctatgcag atcaacttgg tcaacgccct ggtgctggtg ctgtgtactt
    17941 aaatatcttc cattatgatg tagaagaatt tttagatact aaaaaagtaa atgcggatga
    18001 agatttacgt ttatctacaa tatcaactgg tttaattgtt ccatctaaat tcttcgattt
    18061 agctaaagaa ggtaaggact tttatatgtt tgcacctcat acagttaaag aagaatatgg
    18121 tgtgacatta gacgatatcg atttagaaaa atattatgat gacatggttg caaacccaaa
    18181 tgttgagaaa aagaaaaaga atgcgcgtga aatgttgaat ttaattgcgc aaacacaatt
    18241 acaatcaggt tatccatatt taatgtttaa agataatgct aacagagtgc atccgaattc
    18301 aaacattgga caaattaaaa tgagtaactt atgtacggaa attttccaac tacaagaaac
    18361 ttcaattatt aatgactatg gtattgaaga cgaaattaaa cgtgatattt cttgtaactt
    18421 gggctcatta aatattgtta atgtaatgga aagcggaaaa ttcagagatt cagttcactc
    18481 tggtatggac gcattaactg ttgtgagtga tgtagcaaat attcaaaatg caccaggagt
    18541 tagaaaagct aacagtgaat tacattcagt tggtcttggt gtgatgaatt tacacggtta
    18601 cctagcaaaa aataaaattg gttatgagtc agaagaagca aaagattttg caaatatctt
    18661 ctttatgatg atgaatttct actcaatcga acgttcaatg gaaatcgcta aagagcgtgg
    18721 tatcaaatat caagactttg aaaagtctga ttatgctaat ggcaaacatt tcgagttcta
    18781 tacaactcaa gaatttgaac ctcaattcga aaaagtacgt gaattattcg atggtatggc
    18841 tattcctact tctgaggatt ggaagaaact acaacaagat gttgaacaat atggtttata
    18901 tcatgcatat agattagcaa ttgctccaac acaaagtatt tcttatgttc aaaatgcaac
    18961 aagttctgta atgccaatcg ttgaccaaat tgaacgtcgt acttatggta atgcggaaac
    19021 attttaccct atgccattct tatcaccaca aacaatgtgg tactacaaat cagcattcaa
    19081 tactgatcag atgaaattaa tcgatttaat tgcgacaatt caaacgcata ttgaccaagg
    19141 tatctcaacg atcctttatg ttaattctga aatttctaca cgtgagttag caagattata
    19201 tgtatatgcg cactataaag gattaaaatc actttactat actagaaata aattattaag
    19261 tgtagaagaa tgtacaagtt gttctatcta acaattaaat gttgaaaatg acaaacagct
    19321 aatcatctgg tctgaattag cagatgatta gactgctatg tctgtatttg tcaattattg
    19381 agtaacatta caggaggaaa ttatattcat gatagctgtt aattggaaca cacaagaaga
    19441 tatgacgaat atgttttgga gacaaaatat atctcaaatg tgggttgaaa cagaatttaa
    19501 agtatcaaaa gacattgcaa gttggaagac tttatctgaa gctgaacaag acacatttaa
    19561 aaaagcatta gctggtttaa caggcttaga tacacatcaa gcagatgatg gcatgccttt
    19621 agttatgcta catacgactg acttaaggaa aaaagcagtt tattcattta tggcgatgat
    19681 ggagcaaata cacgcgaaaa gctattcaca tattttcaca acactattac catctagtga
    19741 aacaaactac ctattagatg aatgggtttt agaggaaccc catttaaaat ataaatctga
    19801 taaaattgtt gctaattatc acaaactttg gggtaaagaa gcttcgatat acgaccaata
    19861 tatggccaga gttacgagtg tatttttaga aacattctta ttcttctcag gtttctatta
    19921 tccactatat cttgctggtc aagggaaaat gacgacatca ggtgaaatca ttcgtaaaat
    19981 tcttttagat gaatctattc atggtgtatt taccggttta gatgcacagc atttacgaaa
    20041 tgaactatct gaaagtgaga aacaaaaagc agatcaagaa atgtataaat tgctaaatga
    20101 cttgtattta aatgaagagt catacacaaa aatgttatac gatgatcttg gaatcactga
    20161 agatgtgcta aactatgtta aatataatgg aaacaaagca ctttcaaact taggctttga
    20221 accttatttt gaggaacgtg aatttaaccc aatcattgag aatgccttag atacaacaac
    20281 taaaaaccat gacttcttct cagtaaaagg tgatggttat gtattagcat taaacgtaga
    20341 agcattacaa gatgatgact ttgtatttga caacaaataa caattaaatt aaaagacctt
    20401 cacatgtaaa gggaaatagc gattcgtttc gtcttgtctc ctacatgttg aaggtctttt
    20461 ctctatgctt aaaagcgtat gcatattttg aaaaagtaaa aattgtattt aatgatgtca
    20521 atgaggctag aaagtataca agatattgca ttacaaaagg gtgttctatt ttactttaag
    20581 caaacaaaat acacattccg aaaaattaaa tttcagttta attgcaaata tcaataaaat
    20641 tgacactaaa ttatttgaaa ggctattgaa attatggtca aaaaacgcta ctattaatga
    20701 gaaatattat caatgataat gattatcatt aatttaaagg gagaaaaatt tgtaatgaag
    20761 tatttattaa agggaaatat tttgcttcta ttactaatat tgttgacaat tatttcgttg
    20821 ttcataggtg tgagtgaact atcaattaaa gatttactac atttaactga atcacagcgg
    20881 aatattttat tctcaagccg aataccaagg acgatgagta ttttaattgc tggaagttcg
    20941 ttggctttag caggcttgat aatgcaacaa atgatgcaaa ataagtttgt tagtccgact
    21001 acagctggaa cgatggaatg ggctaaacta ggtattttaa ttgctttatt gttctttcca
    21061 accggtcata ttttattaaa actagtattt gctgttattt gcagtatttg cggtacgttt
    21121 ttatttgtta aaatcattga ttttataaaa gtgaaagatg tcatttttgt accgctttta
    21181 ggaattatga tgggtgggat tgttgcaagt ttcacaacct tcatctcatt gcgcacgaat
    21241 gctgttcaaa gcattggtaa ctggcttaac gggaactttg ccattatcac aagtggacgc
    21301 tatgaaattt tatatttaag tattcctctt ttagcattga catatctttt tgctaatcat
    21361 ttcacgattg taggaatggg taaagacttt actaataatt taggtttgag ttacgaaaaa
    21421 ttaattaaca tcgcattgtt tattactgca actattacag cattggtagt ggtgactgtt
    21481 ggaacattac cgttcttagg actagtaata ccaaatatta tttcaattta tcgaggtgat
    21541 catttgaaaa atgctatccc tcatacgatg atgttaggtg ccatctttgt attattttct
    21601 gatatagttg gcagaattgt tgtttatcca tatgaaataa atattggttt aacaataggt
    21661 gtatttggaa caatcatttt ccttatcttg cttatgaaag gtaggaaaaa ttatgcgcaa
    21721 caataataaa aaaataatgc ttttaattgc agtaacgtta ttaattagta tgctgtactt
    21781 atttgtaggt attgattttg aaatatttga atatcaattt tcaagtcgtt taagaaagtt
    21841 catattaatt attttagtag gtgctgccat tgcaacttca gtggtgattt ttcaagcgat
    21901 tacaaataac cgtctattga caccatcaat aatggggtta gatgcagttt atttatttat
    21961 caaagtattg ccagtctttt tatttggaat tcaatcggta tgggttacta atgtatattt
    22021 gaactttata ttaacactta taacgatggt gttattcgca ctaatcctat tccaaggtat
    22081 ctttaaaatt ggacattttt caatttattt tatcttactt attggtgtcc ttttaggaac
    22141 attttttaga agcataacag gttttattca actgattatg gatcctgagt catttttagc
    22201 aatacaaagt agtatgtttg ctaattttaa tgcttctaat tcgaatttag ttactttctc
    22261 agcagtgcta ttagtaatct tattagtcat tacaatttta ctattgcctt atttagatgt
    22321 attgctttta ggtcgtgctg aagcaattaa tcttgggata tcgtatgaaa aattaacgcg
    22381 aattctactt gtaatagtct cagttttagt ttctgtgtca actgcattag taggaccaat
    22441 tacattttta ggtttattaa ctgtaaatct agcgcatgaa ctaatgaaga cgtatgaaca
    22501 taagtatatt ttaattgcga caatttgctt gagttggatt agtttattta gtgcgcaatg
    22561 ggtagttgaa aatgtgtttg aagctacgac agaaatgagt atacttattg atttgattgg
    22621 tggaagttat ttcatttatc tattagttag aaggagaaat gcgcaatgat tcaagttgaa
    22681 aatttaacta aaactataaa taatcaaatg atattggaag atattagcat agatatcgaa
    22741 aaaggtaaat tgacttcttt aattggacct aatggtgcgg gtaagagtac tttactttca
    22801 gcgatatgta ggttaattcg ttttgataac ggtgaagtga aaatagatgg acagctcatg
    22861 tctgattata aaaataatga cttgtcgaaa aaaatatcta tattaaaaca aacaaaccat
    22921 actgaaatga atattacggt agagcagttg gtaaactttg gacgattccc ttattctaaa
    22981 ggtcgtttga cgaaagagga tcatgatatt gtcaatgatg cgctagattt gttgcaacta
    23041 caagatatca gaaatcgtaa tattaagtca ttatctggtg gacaacgtca gcgtgcatac
    23101 attgcaatga caatagcaca agatactgaa tatattttgc tagatgaacc attaaataat
    23161 ttagatatga agcatgctgt tcaaattatg caaacgttaa aaatgttagc gcataaaatg
    23221 aataaagcga ttgtcattgt gttacatgat attaactttg cgtcctgtta ttcagatcag
    23281 attgtagcat tgaaaaacgg acaactagtt aagtcagatt tgaaagataa tgtcattcaa
    23341 agtagtgttt taagtgattt atatgacatg aatattcaaa ttgaacatat aagaaatcaa
    23401 aggatttgtt tatattttaa ggattgataa tttggagaca ctttaaaggg gtgatgcgcc
    23461 aattaaagaa gggttaaacg taaagcattt atttatattt cacatcaagc acacagatta
    23521 agccaaaaga ggagaatatt atattatgaa gaaaacagtc ttatatttag tattagcagt
    23581 aatgttttta ttagcggcat gcggtaacaa ttctgataaa gaacaatcaa aatcagaaac
    23641 taaaggttct aaagatacag taaaaattga aaataactat aaaatgcgtg gcgagaaaaa
    23701 agatggtagt gacgctaaaa aagttaaaga aactgttgaa gtaccaaaaa atcctaaaaa
    23761 tgcagttgtg ttagactatg gcgcattaga tgtaatgaaa gaaatgggct tatcagataa
    23821 agtaaaagca ttacctaaag gggaaggcgg taagtcatta ccgaatttct tagaatcatt
    23881 taaagatgat aaatatacaa acgttggtaa tttaaaagaa gtgaattttg ataaaattgc
    23941 tgcgacgaaa cccgaagtaa tctttatctc tggacgtaca gctaatcaaa agaatttaga
    24001 tgaattcaaa aaagctgcac ctaaagcgaa aattgtttat gttggtgcag atgaaaagaa
    24061 cttaattggt tcaatgaaac aaaacactga aaatatcggt aaaatttacg ataaagaaga
    24121 taaagctaaa gaattaaata aagatttaga taacaaaatt gcttcaatga aagataaaac
    24181 gaaaaacttc aataaaactg ttatgtattt actagttaac gaaggtgaat tatcaacatt
    24241 tggacctaaa ggtcgttttg gtggattagt ttacgataca ttaggattca atgcagttga
    24301 taaaaaagta agtaatagca atcatggaca aaatgtttct aacgaatatg ttaataaaga
    24361 aaatccagat gttattttag cgatggatag aggtcaagcg ataagtggta aatcaactgc
    24421 gaaacaagca ttaaataatc ctgtattaaa aaatgttaaa gcaattaaag aagacaaagt
    24481 atataattta gatcctaaat tatggttctt tgcagctgga tcaactacaa ctacaattaa
    24541 acaaattgag gaacttgata aagttgtaaa ataattttaa aagaggggaa caatggttaa
    24601 aggtcttaat cattgctccc ctcttttctt taaaaaagga aatctgggac gtcaatcaat
    24661 gtcctagact ctaaaatgtt ctgttgtcag tcgttggttg aatgaacatg tacttgtaac
    24721 aagttcattt caataccagt gggctccaaa catagagaaa tttgattttc aatttctact
    24781 gacaatgcaa gttggcgggg cccaaacata gagaatttca aaaaggaatt ctacagaagt
    24841 ggtgctttat catgtctgac ccactcccta taatgttttg actatgttgt ttaaatttca
    24901 aaataaatat gatagtgata tttacagcga ttgttaaacc gagattggca atttggacaa
    24961 cgctctacca tcatatattc attgattgtt aattcgtgtt tgcatacacc gcataagatt
    25021 gctttttcgt taaatgaagg ctcagaccaa cgcttaatgg cgtgcttttc aaactcatta
    25081 tggcacttat agcatggata gtatttatta caacatttaa atttaatagc aataatatct
    25141 tcttcggtaa aataatggcg acagcgtgtt tcagtatcga ttaatgaacc ataaacttta
    25201 ggcatagaca aagctcctta acttacgatt cctttggatg ttcaccaata atgcgaactt
    25261 cacgatttaa ttcaatgcca aatttttctt tgacggtctt ttgtacataa tgaataaggt
    25321 tttcataatc tgtagcagtt ccattgtcta catttaccat aaaaccagcg tgtttggttg
    25381 aaacttcaac gccgccaata cggtgacctt gcaaattaga atcttgtatc aatttacctg
    25441 caaaatgacc aggcggtctt tggaatacac taccacatga aggatactct aaaggttgtt
    25501 tagattctct acgttctgtt aaatcatcca ttttagcttg tatttcagtc attttaccag
    25561 gagctaaagt aaatgcagct tctaatacaa ctaagtgttc tttttgaata atgctattac
    25621 gataatctaa ctctaattct tttgttgtaa gtttaattaa cgagccttgt tcgtttacgc
    25681 aaagcgcata gtctatacaa tctttaactt cgccaccata agcgccagca ttcatataca
    25741 ctgcaccacc aattgaacct ggaataccac atgcaaattc aaggccagta agtgcgtaat
    25801 cacgagcaac acgtgagaca tcaataattg cagcgccgct accggctatt atcgcatcat
    25861 cagatacatc gatatgatct agtgataata aactaattac aatgccgcga ataccacctt
    25921 cacggataat aatatttgag ccatttccta aatatgtaac aggaatctca ttttgatagg
    25981 catatttaac aactgcttgt acttcttcat ttttagtagg ggtaatataa aagtcggcat
    26041 taccacctgt tttagtataa gtgtatcgtt ttaaaggttc atcaacttta attttttcat
    26101 ttgggataag ttgttgtaaa gcttgataga tgtctttatt tatcacttct cagtacatcc
    26161 tttctcatgt ctttaatatc atatagtatt ataccaattt taaaattcat ttgcgaaaat
    26221 tgaaaagaaa gtattagaat tagtataatt ataaaatacg gcattattgt cgttataagt
    26281 attttttaca tagtttttca aagtattgtt gcttttgcat ctcatattgt ctaattgtta
    26341 agctatgttg caatatttgg tgcttttttg tattgaattg caaagcaata tcatcattag
    26401 ttgataagag gtaatcaagt gcaagataag attcaaatgt ttgggtattc atttgaatga
    26461 tatgtagacg cacctgttgt tttagttcat gaaaattgtt aaacttcgcc atcataactt
    26521 tcttagtata tttatgatgc aaacgataaa accctacata atttaagcgt ttttcatcta
    26581 aggatgtaat atcatgcaaa ttttctacac ctactaaaat atctaaaatt ggctctgttg
    26641 aatatttaaa atgatgcgta ccgccaatat gttttgtata ttttactggg ctgtctaaga
    26701 ggttgaataa taatgattca atttcagtgt attgtgattg aaaacaatta gttaaatcac
    26761 tattaatgaa tggttgaaca tttgaataca tgataaactc ctttgatatt gaaaattaat
    26821 ttaatcacga taaagtctgg aatactataa cataattcat tttcataata aacatgtttt
    26881 tgtataatga atctgttaag gagtgcaatc atgaaaaaaa ttgttattat cgctgtttta
    26941 gcgattttat ttgtagtaat aagtgcttgt ggtaataaag aaaaagaggc acaacatcaa
    27001 tttactaagc aatttaaaga tgttgagcaa aaacaaaaag aattacaaca tgtcatggat
    27061 aatatacatt tgaaagaaat tgatcatcta agtaaaactg atacaactga taaaaatagt
    27121 aaagaattta aggcactaca agaagatgtt aaaaaccatc tcatacctaa atttgaagca
    27181 tattataagt cagcaaaaaa tttgcctgat gatacaatga aagttaagaa attaaaaaaa
    27241 gaatatatga cgcttgcaaa tgagaagaag gatgcgatat atcaattaaa aaaattcata
    27301 ggtttatgta atcaatctat caagtataac gaagacattt tagattatac gaaacaattt
    27361 gaaaaaaata gatacaaagt tgaatcagaa attaaattag ctgataataa aagtgaagca
    27421 actaatctta cgacaaaatt agaacataat aataaagcgt taagagatac tgcgaagaag
    27481 aacctagatg atagtaaaga aaatgaagta aaaggcgcga ttaaaaatca cattatgcca
    27541 atgattgaaa aacaaattac cgatattaac caaactaata ttagtgataa gcatgttaat
    27601 aatgcaagga aaaacgcaat agaaatgtat tacagtctgc agaactatta taatacacgt
    27661 attgaaacaa taaaggttag tgagaagtta tcaaaagtcg atgtagataa gttgccgaaa
    27721 aagggtatag atataactca cggcgataaa gcctttgaaa aaaagcttga aaaattagaa
    27781 gaaaaataac tataatcatt tttcaaagtt aaaaattttg aatttatggt taacatgtca
    27841 acttactatg tgtataatgg taaacattga tattaactat atgtataaaa atgtcacgca
    27901 gatgctattt aaatgtgata aatattttta gaggtgaata gagtggttat aaagctaagt
    27961 tcaattgacc aatttgaaca ggttattgag gaaaataaat atgtttttgt attaaaacat
    28021 agtgaaactt gtccaatatc ggcaaatgcg tacgatcaat ttaataaatt tttatatgaa
    28081 cgcgatatgg acggttatta tttgattgtc caacaagaac gcgatttgtc agattatatt
    28141 gctaaaaaaa cgaacgttaa acatgaatca cctcaagcat tttattttgt aaatggtgaa
    28201 atggtttgga atcgagacca cggtgatatc aatgtgtcgt cattagcaca agcagaagaa
    28261 taatgaaact ataggttgga acattttgcc ttacactact agacgtgaat agcacaactt
    28321 aaattcgtgt gaatcagagt agtttggcta taatgatgtt ctgacctttt attttatgtc
    28381 acctttagaa gcagttaagt tagtactttt ttacaaacat atgtataatg tattcgagta
    28441 tttttattga aaatattttg gaaaacgacg aatccaataa gaaaatttaa acatgatttg
    28501 taagttagtt taataggaaa tatatgataa accaaaagaa gcatactgtt atttactgga
    28561 ataattaata atcatgtcat gttaaatgtt cgcatataat cacgagataa aatctaaaat
    28621 ttaagattaa tcttttatga ataaaaaacg tatcacaaca aataataaag taaggtggtc
    28681 aaggttatga aagtattagt agccatggat gagtttcatg gaattatttc gagttatcaa
    28741 gctaatagat atgttgaaga ggcagttgca agccaaattg aaactgcaga tgtagttcaa
    28801 gtaccattgt ttaatggaag acatgaatta ttagattctg tatttttatg gcaatctggg
    28861 caaaagtatc gtataccagt acatgatgca gatatgaatg aagttgaagg tgtttacgga
    28921 caaactgata cagggatgac cgttatcgag gggaatttat ttttaaaagg taaaaaacca
    28981 attgttgaac gaacaagtta tggtttagga gaaatgatta aacatgcatt agataatgac
    29041 gcaaaacatg ttgtaatttc actaggtggg attgatagtt ttgatgctgg tgcaggtatg
    29101 ttacaagcat taggtgctca attctatgat gacgaagggc gtgtcgtaga tatgagacaa
    29161 ggtgctggtg taattaaata tattcgtcgt atggatatgt cgaacttaca ccctaaaatg
    29221 gaaacagcaa gaattcaagt aatgtcggat ttttcaagtc gattatatgg taagcaaagt
    29281 gaaatcatgc aaacttatga tgcgcatcag ttgcatcata atcaagcagc agaaatcgat
    29341 aatttaattt ggtattttag tgagttattt aaaagtgaat tgaaaattgc aattggtcca
    29401 gttgaacgtg gtggtgctgg tggtggaatt gcagcagtct tgaatggact gtatcaagct
    29461 gaaatattaa ccagtcatgc attagtagac caactaacac atttagaaaa tttagttgaa
    29521 caagcggatt taattatttt tggagaagga ttaaatgaaa atgatcagtt gctagaaacg
    29581 acaacattgc gtattgcaga actttgtcat aaacatcaaa aggttgccat tgcaatttgt
    29641 gcaactgctg aaaagtttga tttatttgaa tcacaagggg ttacagcaat gtttaataca
    29701 tttatcgata tgccagaaac ttatactgac tttaaaatgg gattacaaat taggcattat
    29761 acggttcagt ctttaaaact gttgaaaaca cattttaatg ttgaggttta gtaaagaagg
    29821 actaaattga tgatgctgtc atgatggtta ataacattta tgatggttag caaaacgaat
    29881 tagaagatcg aaagtatacg taaaaaatat gaaaaatcac gctatcattg cactgaatgt
    29941 tagcgtgatt tttttattaa ttaagcctga gttgaactag tatataatcg tttgttttta
    30001 gtgattttca gcgatatctt ctacaattcc aatgattact tgtactgctt tttccataac
    30061 atcaatggat gcatattcat atgggccgtg gaagttaccg caacctgtaa agatgtttgg
    30121 agttggtaac cccataaatg acaattgtga accatctgta ccaccacgaa taggttcagt
    30181 gtttgctgga atatctaatt tggcaaagac acgtttaggt atatcaataa tatgaggcaa
    30241 tggtaatatt ttttctgcca tattgaaata ttgatccgat atatcaactt taactggata
    30301 attttcaaaa tgggcattga tatcgtcacg tatttctaaa atacgtttct tacgcaattc
    30361 gaattgtttt ttatcatgat cacgaataat gtattgcaaa gttgcttttt caacagttcc
    30421 ttcaaagttc attaagtgat aaaagccttc gtatccttct gttcgctccg gaacttcact
    30481 atcaggtagc aaactatcga attgttcacc taaacgtatt gcgtttacca ttgcattttt
    30541 agctgaacca ggatgaacat ttacaccgtg gcatgtaata accgcttcag cagcgttaaa
    30601 gctttcatat tgtaattctc catattgact accatccata gtataagcaa aatcagcatt
    30661 gaagcggtca acatcaaatt tatgtggacc acgaccgatt tcttcgtctg gtgtaaatcc
    30721 aatgcgaatg gtaccatgtt taatttctgg atgttcttgt aaataacaaa tagcttccat
    30781 aatttccaca atacccgctt tatcgtctgc acctagtaac gatgtaccat cagttaccat
    30841 taatgtatga ccaactaaac tgttaagttc tggaaatact ttaggatcta agacacgttt
    30901 agtattgcct agtttgtatg gcttaccatc atagttttca ataatttgcg gtttaacatt
    30961 tgaagcattg aaatcaggtg atgtatcaac atgcgccaaa aatccaactg ttgggacgtc
    31021 ggcatcgatg ttactttcta atgtagcaaa taagtagcca ttttcatcta aatcagttgg
    31081 caatcctaat tgttgtaatt ctttttctaa taaatgtaac aaatcccatt gcttttcagt
    31141 tgaaggtgtt gttgtagatt ttggatcaga ttgcgtatca attgtcgtat atcttgttaa
    31201 tctatctatc aattggttct tcattatatt cgacccctta aactctatta ttcatgttgt
    31261 aagatttttt atatgtctta cctttgattt taccatacag ttgtttgata cgtgtgtata
    31321 ggtaatatag aatttcagaa actaatatac cgaaagcaat cgcacctgaa atcagtgtaa
    31381 cttctaaaaa tgtatttaca gcacttgtat aatcattcga tactaaaaaa cgagtcgctt
    31441 gataagctgc accaccaggt actaatggta taatgcctgg tactatgaat ataattaccg
    31501 gtcgtttata tctgcgactc atagtatgac tcattaagcc taaaattaag cttcccaaaa
    31561 atgaagcgcc aacttttcca aactctaaat ctaccgttaa ttggtaaatc gtccatgcaa
    31621 tggcacctac aaatccacat gctactaaga ggcgtttggg tgcattgaaa atgatagaga
    31681 aaagtactgt tgatataaag ctgattgtaa aatgaaataa ataaaatagc atgctttaac
    31741 agtccttcct taaatgatta ataaaacgat tgcgacacca gcaccgattg cgaatgctgt
    31801 taatgcagct tcaacacctc gagacatacc tgcaagtaat tcacccgcta ataaatctcg
    31861 aatggcattg gtaattaata taccagggac aagtggcatg acactggcta tagtaatgat
    31921 atcttgattg gttgcaatgc ctaatttagt aaatgtggct gcaatggata tgaccacagc
    31981 agctgcaaca aactctgaga aaaatttaat ttgtatatag cgttgcacaa agctgaatgt
    32041 taaaaatgcg gatccaccag caatgactgc aatccaacaa tctgatgcga caccaccaaa
    32101 cataaatagg aagaagccac atgcaatggc agctgcaaag aaattcgtta aaaaagaata
    32161 ttgtaatgat gcatgctgta aatgaataaa ttcagattta gcttcatcaa ttgtgagttc
    32221 tttatttgat attttacgtg aaagactatt cgttaaagcg attttctcta aatctgttgt
    32281 acgctcttgt acacgaatta atcttgtact tgttcgatcg tttaatgaaa aaataattgc
    32341 agttgaactg acaaaactat atgtattatg aagaccataa ctatgtgcga tacggttcat
    32401 tgtatcttca actcgatatg tttcagcacc tgattcaagt aaaattctac ctgcaattaa
    32461 tacaacatca atcactttgt tttcatctat aattgtgatt gaatctggca tatcaattca
    32521 cctccaatga tatgtgttat ttatttgaac aattgaagtt tacaacttgt tgttacaact
    32581 ttcaatagtg agactttgtg ttagtatgat gaacttgtat ggttcaaatt taaataagaa
    32641 aaactgttaa tctttgctat tatactatga tttaataata gcaaaggatt aacagttttg
    32701 tcgttgttat aaattgataa tagggttaaa cattactttg tttcgccctt gatttttggc
    32761 tacatgcacc atatcgtctg catctttaaa cactttacgc tgtgattttg gatcgtcatc
    32821 tgttaaataa ccaacaccga tagacactga caatttaata acttctttgt ttggtaaatg
    32881 gaatgatgat ttttcaacac ccgaacgaat attttcagct aatttaacac tttgatcaag
    32941 tgaataattg tgaatgacaa ctgagaactc ttcgccacca tttctaaaaa ttttaaattg
    33001 attcggcaca tagtttttaa gtaattgaga catttgtttt aatacagcat cacctgattt
    33061 gtgtgagtag gtatcattga catctttaaa tccatcgata tcgattaata ataaagcgat
    33121 actttgatgt tctttttcag cttttcgtga aatttcattt aaatgtctat caaattcttt
    33181 tacattacct aagcctgtta agtaatcata tttatcttcg ttttcataac gatttacgag
    33241 tgagaagaaa tgccaaatat cgacaaatgt tatcgctgaa gctaaagtga taattaatga
    33301 aattggtatt aaaatgataa cttccgatag tgtataaata ggactcacta acgcgacacc
    33361 aaataaaatg attattgtaa caacattaag tattaataat gatagcacat cattttgttt
    33421 taaaaatggt ccaatagcac ttgttactgc agcaataaca atcaacgtaa caccgtacat
    33481 aatcgagttg ttaaatacta caatttcaac aattgctaca attactgtgg cagataatgt
    33541 atagaccata tttgtaaatc tacctaaaaa caataaagga acgaatgtta agtgaattaa
    33601 ataatcttca cgataaggga tagggtagac agataataat aatgatacga ttgtcattaa
    33661 aacagtgaca taagccttag aaaaaaccat acgtttgttt tctgaatact gtaagcgatg
    33721 gaataaatag attccagcga ctataacaga tatattgtat ataaatgctt cgaacatgtc
    33781 tgaatcgact cctttaattg accactagct attgtaagtg aaaacttaca atttgtcatt
    33841 agtttacata taaaattaat gtatgatata gactttgatg ttaaaatgtt gccttaaatg
    33901 atatgatgaa aaaatgaata ataggcgata tataagaaat gaatcgtata gttgtaaata
    33961 tgatatcatt gattgagcga attaatttat aataaagcta t
//
LOCUS       DANBWH010000006        32813 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.006, whole genome shotgun sequence.
ACCESSION   DANBWH010000006 DANBWH010000000
VERSION     DANBWH010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 32813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32813
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.006"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            65..1483
                     /locus_tag="PPY92_000202"
     CDS             65..1483
                     /locus_tag="PPY92_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDJ2624234.1"
                     /translation="MENTVKYRKFILPIVVGLLIWALTPFKPDAVDPTAWYMFAIFVA
                     TIIACITQPMPIGAVSIIGFTIMVLVGIVDMKTAVAGFGNNSIWLIAMAFFISRGFVK
                     TGLGRRIALHFVKLFGKKTLGLAYSIVGVDLILAPATPSNTARAGGIMFPIIKSLSES
                     FGSKPKDGSARKMGAFLVFTEFQGNLITAAMFLTAMAGNPLAQNLASSTSNVHITWMN
                     WFLAALVPGLVSLIVVPFIIYKIYPPTVKETPNAKSWAENELATMGKIALAEKFMIGI
                     FVIALTLWIVGSFIHIDATLTAFIALALLLLTGVLTWQDILNETGAWNTLVWFSVLVL
                     MADQLNKLGFIPWLSKSIATSLGGLSWPIVLVILILFYFYSHYLFASSTAHISAMYAA
                     LLGVAIAAGAPPLFSALMLGFFGNLLASTTHYSSGPAPILFSSGYVTQKRWWTMNLIL
                     GFVYFIIWIGLGSLWMKVIGIF"
     gene            complement(1897..2805)
                     /gene="rarD"
                     /locus_tag="PPY92_000203"
     CDS             complement(1897..2805)
                     /gene="rarD"
                     /locus_tag="PPY92_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDJ2624235.1"
                     /translation="MNIEYKKGIFLALSAYILWGILPIYWQFVDAIGAFEILAFRIIF
                     SAIFMIFILAVGQKQRNAFQRDMNQLLGKPIQLLAIVVAGYVITLNWGTFIWAVTNGH
                     VLQTSLGYYINPLVSILLALIFLKERFNKFEWLAILFAFIGVLYMTLKIGEFPIVSII
                     LALSFGTYGLLKKVVHIDAISSITIECIVTAPAGLIYVIYLWQQHQMSFGLNMSSFWL
                     LFSGAITAIPLILFSAGAKRIPLSLIGFIQYVGPTIMFVLGIFVFKEPFSIDQLITFI
                     FIWTGIVLYSLSQYIKLKKHPVAKTL"
     gene            3027..3602
                     /locus_tag="PPY92_000204"
     CDS             3027..3602
                     /locus_tag="PPY92_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDJ2624236.1"
                     /translation="MTLPKIGKPATRALNSQGIYTLEAVSQYTKSSLMEMHGVGPKAI
                     SILEQALFQHQLHFKTEVQSSLPFKLTGDVSCNHAPKRQQMIDFIVATAALDIELLRS
                     LVTTEFIWSVPGHFDIYGPQILIQELSNYYNQVASLNIHSNITHGCLGSMHGIEILKT
                     GKEIHFAHFFEFENHKKDAKLSKVTSYIVVA"
     gene            3764..4780
                     /locus_tag="PPY92_000205"
     CDS             3764..4780
                     /locus_tag="PPY92_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HDJ2624237.1"
                     /translation="MTVFKNERLSWLPYIAIVILLHVIGFSFLWIAGKDHHILFGMGI
                     LAYTLGLRHAFDADHIAAIDNTVRKLLQQRKDPSGVGFYFSIGHSSVVFLMAVFLGIS
                     VKWAKDELPHFQDIGGTIGTLVSGFFLVLIGVLNLIILISLINLFAKLRREHIEEAEV
                     DVLLESRGFVSRFVGPYFKLITRSWHVLPLGFLFGLGFDTASEIALLALSSGASQQAI
                     SFIGILSLPILFASGMSLLDTLDGVVMKYAYNWAFFNPIRKIYYNITITAISVMAALV
                     IGMIELLQILADKLDLHGAFWAFIGSIEFDYLGYILVALFLITWLISSLIWKFGRIEH
                     KWSK"
     gene            5023..5817
                     /locus_tag="PPY92_000206"
     CDS             5023..5817
                     /locus_tag="PPY92_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HDJ2624238.1"
                     /translation="MNIAKLENYLQIDSSRYNRPSIEALNYYATRFMLTVPFENIDVQ
                     NSKPISINIDALFNKIVHDKRGGFCYELNTFFKAYLQQKGFNPELMSATIHTPGGGRS
                     LNGSHASLVVSINDVFYVTDVGFGDLPLHAIPITSSEHTQPITDISGTFRAIFNNEDK
                     DIFYVQKFENDHWHTKYEAEFKPKQIEDFNSNIEYNQTHPDSIFVQHLLITMPQSFGR
                     ATMSENHLTLTRNGSSEKFDVTKDNYKHFLEKYFGLNVTINRIEKQ"
     gene            complement(5951..6463)
                     /locus_tag="PPY92_000207"
     CDS             complement(5951..6463)
                     /locus_tag="PPY92_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HDJ2624239.1"
                     /translation="MEKGNQGIKWSSLIMGVLLLMLAVVIFTFPIENFYAITWLIGLF
                     VLINGVIQIIYRRKAKALVGGNQNWILFMGIVDILFGLIVIFNVGASSAFFIYMFAFW
                     FIFSSIAGLFTFTGSGSLKLISVIFNLLGIVFGVILLFNPLMGIVFISTMIAIAFVFV
                     GVIYVVDALA"
     gene            6746..7504
                     /gene="vraD"
                     /locus_tag="PPY92_000208"
     CDS             6746..7504
                     /gene="vraD"
                     /locus_tag="PPY92_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter ATP-binding
                     subunit VraD"
                     /protein_id="HDJ2624240.1"
                     /translation="MTILSVQHVSKTYGKKHTFQALKDINFDIQKGEFVAIMGPSGSG
                     KTTLLNVLSSIDQISSGSVIANGQELNKLNQKALAKFRKESLGFIFQDYSILPTLTVK
                     ENIMLPLSVQKMSKATMEENYKAITTALGIYDLGNKYPSELSGGQQQRTAAARAFVHK
                     PQIIFADEPTGALDSKSANDLLQRLEEMNKSFDTTIVMVTHDPVAASFAERVIMLKDG
                     QIHTQLYQEGRSKQAFYEDIVHLQSVLGGVSNDI"
     gene            7494..9361
                     /gene="vraE"
                     /locus_tag="PPY92_000209"
                     /pseudo
     CDS             7494..9361
                     /gene="vraE"
                     /locus_tag="PPY92_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter permease
                     subunit VraE"
     gene            9453..9644
                     /gene="vraH"
                     /locus_tag="PPY92_000210"
     CDS             9453..9644
                     /gene="vraH"
                     /locus_tag="PPY92_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter activity
                     modulator VraH"
                     /protein_id="HDJ2624241.1"
                     /translation="MKFRELVKQSYEDLKNLTVTWYNVIALAMIVIVLSVVTTPIIGI
                     PAGLLGGGYYLKRREEKGK"
     gene            10036..10236
                     /locus_tag="PPY92_000211"
     CDS             10036..10236
                     /locus_tag="PPY92_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDJ2624242.1"
                     /translation="MNNGTVKWFNAEKGFGFIEQENGGDVFVHFSGIASDGYKTLEEG
                     QKVTFEITEGQRGDQAVNVQTV"
     gene            complement(10354..10923)
                     /locus_tag="PPY92_000212"
     CDS             complement(10354..10923)
                     /locus_tag="PPY92_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDJ2624243.1"
                     /translation="MNLSKQIKKYRERDGYSQEYLAEKLYVSRQSISNWENDKSLPDI
                     HNLLMMCELFNVTLDDLVKGTIPFVPDIKVQRSLNLWTYVMLIFMTLAAILMGPLVVY
                     WNWTWGITVAIILGIGFYASMKIEDLKKVHKMDNYDRIVAFMNGKDPSEVQTTKARNT
                     MTNALSIISAIGILSLIIFLSVYMANKFL"
     gene            complement(11183..11578)
                     /locus_tag="PPY92_000213"
     CDS             complement(11183..11578)
                     /locus_tag="PPY92_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HDJ2624244.1"
                     /translation="MKLTLMKFFVGGFAVLLSYIVSVTLPWKEFGGIFATFPAVFLVS
                     MFITGMQYGDKVAVHVSRGAVFGMTGVLVCILVTWMMLHMTHMWLISIIVGFLSWFIS
                     AVCIFEAVEFIAQKRLEKHSWKAGKSNSK"
     gene            complement(11646..11999)
                     /locus_tag="PPY92_000214"
     CDS             complement(11646..11999)
                     /locus_tag="PPY92_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HDJ2624245.1"
                     /translation="MFSIGSAILHFVIGGIAVALASIIADKVGGKLGGIIATMPAVFL
                     AAIIALALDHRGTQLVEMSMNLSTGAIVGILSCILTVFLTSLYIKHKGYRKGAIFTVV
                     CWFVISLAIFSIRHL"
     gene            complement(12450..13289)
                     /gene="noc"
                     /locus_tag="PPY92_000215"
     CDS             complement(12450..13289)
                     /gene="noc"
                     /locus_tag="PPY92_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HDJ2624246.1"
                     /translation="MKKPFSKLFGLKNKDDIIGHIEEDRNSNVESIQIERIVPNRYQP
                     RQVFEPNKIKELAESIHEHGLLQPIVVRPIEEDMFEIIAGERRFRAIQSLNLPQADVI
                     IRDMDDEETAVVALIENIQRENLSVVEEAEAYKKLLEIGDTTQSELAKSLGKSQSFIA
                     NKLRLLKLAPKVLLRLREGKITERHARAVLSLSDSEQEALIEQVIAQKLNVKQTEDRV
                     RQKTGPEKVKAQNLRFAQDVTQARDEVGKSIQAIQQTGLHVEHKDKDHEDYYEIKIRI
                     YKR"
     gene            complement(13332..14051)
                     /gene="rsmG"
                     /locus_tag="PPY92_000216"
     CDS             complement(13332..14051)
                     /gene="rsmG"
                     /locus_tag="PPY92_000216"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDJ2624247.1"
                     /translation="MTVEWLAEQLKEHNIELTETQKQQFQTYYRLLVEWNEKMNLTSI
                     TDEHDVYLKHFYDSIAPSFYFDFNQPISICDVGAGAGFPSIPLKIMFPQLKVTIVDSL
                     NKRIQFLNHLASELQLQDVSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLP
                     LIKKGGQFVALKSSKGEEELEEAKFAISVLGGNVTETHTFKLPEDAGERQMFIIDKKR
                     QTPKKYPRKPGTPNKTPLLEK"
     gene            complement(14051..15928)
                     /gene="mnmG"
                     /locus_tag="PPY92_000217"
     CDS             complement(14051..15928)
                     /gene="mnmG"
                     /locus_tag="PPY92_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDJ2624248.1"
                     /translation="MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMP
                     CNPSVGGPAKGIVVREIDALGGQMAKTIDKTHIQMRMLNTGKGPAVRALRAQADKVLY
                     QQEMKRVIEDEENLHIMQGMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGE
                     IILGNMKYSSGPNHQLPSITLSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPG
                     DDVGRAFSFETTEYILDQLPCWLTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPS
                     IEDKFVRFNDKPRHQLFLEPEGRNTNEVYVQGLSTSLPEHVQRQMLETIPGLEKADMM
                     RAGYAIEYDAIVPTQLWPTLETKMIKNLYTAGQINGTSGYEEAAGQGLMAGINAAGKV
                     LNTGEKILSRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDMGY
                     ELGMISEERYARFNEKRQQIDAEIKRLSDIRIKPNEHTQAIIEQHGGSRLKDGILAID
                     LLRRPEMTYDIILEILEEEHQLNADVEEQVEIQTKYEGYINKSLQQVEKVKRMEEKKI
                     PEDLDYSKIDSLATEAREKLSEVKPLNIAQASRISGVNPADISILLIYLEQGKLQRVS
                     D"
     gene            complement(15995..17374)
                     /gene="mnmE"
                     /locus_tag="PPY92_000218"
     CDS             complement(15995..17374)
                     /gene="mnmE"
                     /locus_tag="PPY92_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDJ2624249.1"
                     /translation="MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADKLYKGKHLLND
                     VPSHTINYGHIIDPESKEVVEEVMVSVLRAPKTFTREDIIEINCHGGILTINRVLELT
                     MTYGARMAEPGEFTKRAFLNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLI
                     KKQRQSILEILAQVEVNIDYPEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIM
                     REGLSTVIVGKPNVGKSSMLNNLIQDNKAIVTEVAGTTRDVLEEYVNVRGVPLRLVDT
                     AGIRETEDIVEKIGVERSRKALSQADLILFVLNNNEALTQEDYTLYEVVKNEDVIVIV
                     NKMDLEQNIDINEVKDMIGDTPLIQTSMLKQEGIDELEIQIRDLFFGGEVQNQDMTYV
                     SNSRHISLLKQARQTIQDAIDAAESGVPMDMVQIDLTRTWEILGEIIGETASDELIDQ
                     LFSQFCLGK"
     gene            complement(17531..17884)
                     /gene="rnpA"
                     /locus_tag="PPY92_000219"
     CDS             complement(17531..17884)
                     /gene="rnpA"
                     /locus_tag="PPY92_000219"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDJ2624250.1"
                     /translation="MLLEKAYRIKKNADFQRIYKKGHSVANRQFVVYTCNNKEIDHFR
                     LGISVSKKLGNAVLRNKIKRAIRENFKVHKSHILAKDIIVIARQPAKDMTTLQIQNSL
                     EHVLKIAKVFNKKIK"
     gene            complement(18005..18142)
                     /gene="rpmH"
                     /locus_tag="PPY92_000220"
     CDS             complement(18005..18142)
                     /gene="rpmH"
                     /locus_tag="PPY92_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDJ2624251.1"
                     /translation="MVKRTYQPNKRKHSKVHGFRKRMSTKNGRKVLARRRRKGRKVLS
                     A"
     gene            18873..20234
                     /gene="dnaA"
                     /locus_tag="PPY92_000221"
     CDS             18873..20234
                     /gene="dnaA"
                     /locus_tag="PPY92_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDJ2624252.1"
                     /translation="MSEKEIWEKVLEIAQEKLSAVSYSTFLKDTELYTIKDGEAIVLS
                     SIPFNANWLNQQYAEIIQAILFDVVGYEVKPHFITTEELANYSNNETATPKETTKPST
                     ETTEDNHVLGREQFNAHNTFDTFVIGPGNRFPHAASLAVAEAPAKAYNPLFIYGGVGL
                     GKTHLMHAIGHHVLDNNPDAKVIYTSSEKFTNEFIKSIRDNEGEAFRERYRNIDVLLI
                     DDIQFIQNKVQTQEEFFYTFNELHQNNKQIVISSDRPPKEIAQLEDRLRSRFEWGLIV
                     DITPPDYETRMAILQKKIEEEKLDIPPEALNYIANQIQSNIRELEGALTRLLAYSQLL
                     GKPITTELTAEALKDIIQAPKSKKITIQDIQKIVGQYYNVRIEDFSAKKRTKSIAYPR
                     QIAMYLSRELTDFSLPKIGEEFGGRDHTTVIHAHEKISKDLKEDPIFKQEVENLEKEI
                     RNV"
     gene            20512..21645
                     /gene="dnaN"
                     /locus_tag="PPY92_000222"
     CDS             20512..21645
                     /gene="dnaN"
                     /locus_tag="PPY92_000222"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDJ2624253.1"
                     /translation="MMEFTIKRDYFITQLNDTLKAISPRTTLPILTGIKIDAKEHEVI
                     LTGSDSEISIEITIPKTVDGEDIVNISETGSVVLPGRFFVDIIKKLPGKDVKLSTNEQ
                     FQTLITSGHSEFNLSGLDPDQYPLLPQVSRDDAIQLSVKVLKNVIAQTNFAVSTSETR
                     PVLTGVNWLIQENELICTATDSHRLAVRKLQLEDVSENKNVIIPGKALAELNKIMSDN
                     EEDIDIFFASNQVLFKVGNVNFISRLLEGHYPDTTRLFPENYEIKLSIDNGEFYHAID
                     RASLLAREGGNNVIKLSTGDDVVELSSTSPEIGTVKEEVDANDVEGGSLKISFNSKYM
                     MDALKAIDNDEVEVEFFGTMKPFILKPKGDDSVTQLILPIRTY"
     gene            22026..22271
                     /gene="yaaA"
                     /locus_tag="PPY92_000223"
     CDS             22026..22271
                     /gene="yaaA"
                     /locus_tag="PPY92_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDJ2624254.1"
                     /translation="MIILVQEVVVEGDINLGQFLKTEGIIESGGQAKWFLQDVEVLIN
                     GVRETRRGKKLEHQDRIDIPELPEDAGSFLIIHQGEQ"
     gene            22268..23380
                     /gene="recF"
                     /locus_tag="PPY92_000224"
     CDS             22268..23380
                     /gene="recF"
                     /locus_tag="PPY92_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDJ2624255.1"
                     /translation="MKLNTLQLENYRNYDEVTLKCHPDVNILIGENAQGKTNLLESIY
                     TLALAKSHRTSNDKELIRFNADYAKIEGELSYRHGTMPLTMFITKKGKQVKVNHLEQS
                     RLTQYIGHLNVVLFAPEDLNIVKGSPQIRRRFIDMELGQISAVYLNDLAQYQRILKQK
                     NNYLKQLQLGQKKDLTMLEVLNQQFAEYAMKVTDKRAHFIQELESLAKPIHAGITNDK
                     EALSLNYLPSLKFDYAQNEAARLEEIMSILSDNMQREKERGISLFGPHRDDISFDVNG
                     MDAQTYGSQGQQRTTALSIKLAEIELMNIEVGEYPILLLDDVLSELDDSRQTHLLSTI
                     QHKVQTFVTTTSVDGIDHEIMNNAKLYRINQGEIIK"
     gene            23390..25324
                     /gene="gyrB"
                     /locus_tag="PPY92_000225"
     CDS             23390..25324
                     /gene="gyrB"
                     /locus_tag="PPY92_000225"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDJ2624256.1"
                     /translation="MVTALSDVNNTDNYGAGQIQVLEGLEAVRKRPGMYIGSTSERGL
                     HHLVWEIVDNSIDEALAGYANKIEVVIEKDNWIKVTDNGRGIPVDIQEKMGRPAVEVI
                     LTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSQDLEVYVHRNETIYHQAYKKGVPQF
                     DLKEVGTTDKTGTVIRFKADGEIFTETTVYNYETLQQRIRELAFLNKGIQITLRDERD
                     EENVREDSYHYEGGIKSYVELLNENKEPIHDEPIYIHQSKDDIEVEIAIQYNSGYATN
                     LLTYANNIHTYEGGTHEDGFKRALTRVLNSYGLSSKIMKEEKDRLSGEDTREGMTAII
                     SIKHGDPQFEGQTKTKLGNSEVRQVVDKLFSEHFERFLYENPQVARTVVEKGIMAARA
                     RVAAKKAREVTRRKSALDVASLPGKLADCSSKSPEECEIFLVEGDSAGGSTKSGRDSR
                     TQAILPLRGKILNVEKARLDRILNNNEIRQMITAFGTGIGGDFDLAKARYHKIVIMTD
                     ADVDGAHIRTLLLTFFYRFMRPLIEAGYVYIAQPPLYKLTQGKQKYYVYNDRELDKLK
                     SELNPTPKWSIARYKGLGEMNADQLWETTMNPEHRALLQVKLEDAIEADQTFEMLMGD
                     VVENRRQFIEDNAVYANLDF"
     gene            25361..28030
                     /gene="gyrA"
                     /locus_tag="PPY92_000226"
     CDS             25361..28030
                     /gene="gyrA"
                     /locus_tag="PPY92_000226"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDJ2624257.1"
                     /translation="MAELPQSRINERNITSEMRESFLDYAMSVIVARALPDVRDGLKP
                     VHRRILYGLNEQGMTPDKSYKKSARIVGDVMGKYHPHGDLSIYGAMVRMAQDFSYRYP
                     LVDGQGNFGSMDGDGAAAMRYTEARMTKITLELLRDINKDTIDFIDNYDGNEREPSVL
                     PARFPNLLANGASGIAVGMATNIPPHNLTELINGVLSLSKNPDISIAELMEDIEGPDF
                     PTAGLILGKSGIRRAYETGRGSIQMRSRAVIEERGGGRQRIVVTEIPFQVNKARMIEK
                     IAELVRDKKIDGITDLRDETSLRTGVRVVIDVRKDANASVILNNLYKQTPLQTSFGVN
                     MIALVNGRPKLINLKEALVHYLEHQKTVVRRRTQYNLRKAKDRAHILEGLRIALDHID
                     EIISTIRESDTDKVAMESLQQRFKLSEKQAQAILDMRLRRLTGLERDKIEAEYNELLN
                     YISELETILADEEVLLQLVRDELTEIRDRFGDDRRTEIQLGGFEDLEDEDLIPEEQIV
                     ITLSHNNYIKRLPVSTYRAQNRGGRGVQGMNTLEEDFVSQLVTLSTHDHVLFFTNKGR
                     VYKLKGYEVPELSRQSKGIPVVNAIELENDEVISTMIAVKDLESEDNFLVFATKRGVV
                     KRSALSNFSRINRNGKIAISFREDDELIAVRLTSGQEDILIGTSHASLIRFPESTLRP
                     LGRTATGVKGITLREGDEVVGLDVAHANSVDEVLVVTENGYGKRTPVNDYRLSNRGGK
                     GIKTATITERNGNVVCITTVTGEEDLMIVTNAGVIIRLDVADISQNGRAAQGVRLIRL
                     GDDQFVSTVAKVKEDAEDETNEDEQSTSTVSEDGTEQQREAVVNDETPGNAIHTEVID
                     SEENDEDGRIEVRQDFMDRVEEDIQQSSDEDEE"
     gene            complement(28118..28930)
                     /locus_tag="PPY92_000227"
     CDS             complement(28118..28930)
                     /locus_tag="PPY92_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HDJ2624258.1"
                     /translation="METLNSINIPKRKEDSHKGDYGKILLIGGSANLGGAIMLAARAC
                     VFSGSGLITVATHPTNHSALHSRCPEAMVIDINDTKMLTKMIEMTDSILIGPGLGVDF
                     KGNNAITFLLQNIQPHQNLIVDGDAITIFSKLKPQLPTCRVIFTPHLKEWERLSGIPI
                     EEQTYERNREAVDRLGATVVLKKHGTEIFFKDEDFKLTIGSPAMATGGMGDTLAGMIT
                     SFVGQFDNLKEAVMSATYTHSFIGENLAKDMYVVPPSRLINEIPYAMKQLES"
     gene            29256..30770
                     /gene="hutH"
                     /locus_tag="PPY92_000228"
     CDS             29256..30770
                     /gene="hutH"
                     /locus_tag="PPY92_000228"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HDJ2624259.1"
                     /translation="MTLYLDGETLTIEDIKSFLQQQSKIEIIDDALERVKKSRAVVER
                     IIENEETVYGITTGFGLFSDVRIDPTQYNELQVNLIRSHACGLGEPFSKEVALVMMIL
                     RLNTLLKGHSGATLELVRQLQFFINERIIPIIPQQGSLGASGDLAPLSHLALALIGEG
                     KVLYRGEEKDSDDVLRELNRQPLNLQAKEGLALINGTQAMTAQGVISYIEAEDLGYQS
                     EWIAALTHQSLNGIIDAYRHDVHSVRNFQEQINVAARMRDWLEGSTLTTRQAEIRVQD
                     AYTLRCIPQIHGASFQVFNYVKQQLEFEMNAANDNPLIFEEANETFVISGGNFHGQPI
                     AFALDHLKLGVSELANVSERRLERLVNPQLNGDLPAFLSPEPGLQSGAMIMQYAAASL
                     VSENKTLAHPASVDSITSSANQEDHVSMGTTAARHGYQIIENARRVLAIECVIALQAA
                     ELKGVEGLSPKTRRKYEEFRSIVPSITHDRQFHKDIEAVAQYLKQSIYQTTACH"
     misc_binding    30843..31059
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            31149..32435
                     /gene="serS"
                     /locus_tag="PPY92_000229"
     CDS             31149..32435
                     /gene="serS"
                     /locus_tag="PPY92_000229"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDJ2624260.1"
                     /translation="MLDIRLFRNEPDTVKSKIELRGDDPKVVDEILELDEQRRKLISA
                     TEEMKARRNKVSEEIALKKRNKENADDVIAEMRTLGDDIKEKDSQLNEIDNKMTGILC
                     RIPNLISDDVPQGESDEDNVEVKKWGTPREFSFEPKAHWDIVEELKMADFDRAAKVSG
                     ARFVYLTNEGAQLERALMNYMITKHTTQHGYTEMMVPQLVNADTMYGTGQLPKFEEDL
                     FKVEKEGLYTIPTAEVPLTNFYRNEIIQPGVLPEKFTGQSACFRSEAGSAGRDTRGLI
                     RLHQFDKVEMVRFEQPEDSWNALEEMTTNAEAILEELGLPYRRVILCTGDIGFSASKT
                     YDIEVWLPSYNDYKEISSCSNCTDFQARRANIRFKRDKAAKPELAHTLNGSGLAVGRT
                     FAAIVENYQNEDGTVTIPEALVPFMGGKTQISKPVK"
BASE COUNT        11064 a         5538 c         5539 g        10672 t
ORIGIN      
        1 tttgtagtta gctttattct taaattgaaa tatgtataaa acatagaaag aaggacgttt
       61 taacatggaa aacacggtta aatatcgtaa gtttatactc cctatcgttg taggtctcct
      121 tatttgggca cttacacctt ttaaaccgga tgctgtggat ccaacagcat ggtatatgtt
      181 cgcaatattc gtcgcgacaa tcattgcttg tattacacaa ccgatgccaa ttggggccgt
      241 ctctataatt ggatttacaa tcatggtact cgttggcatt gttgacatga aaacggctgt
      301 cgctggtttt ggtaataata gcatttggtt aattgctatg gcatttttca tttcgagagg
      361 atttgtgaaa acaggtcttg gtagacggat cgcacttcat ttcgtcaaat tatttggtaa
      421 aaaaacatta ggcttagcat attctatcgt cggtgtagat ttaattctag cgcctgctac
      481 accgagtaat accgcgcgtg ctggtggaat catgttccca attatcaaat cactttctga
      541 atcatttggt tcgaaaccga aagacggatc agcacgcaaa atgggtgcat ttcttgtttt
      601 cacagaattc caaggtaatt taattactgc ggctatgttt ttaactgcaa tggccggtaa
      661 cccccttgca caaaatttag catctagcac atctaatgtt cacattacat ggatgaattg
      721 gttcctagct gctttagttc ctggacttgt ttccttaatt gttgtacctt ttattattta
      781 taaaatttat ccaccaactg ttaaagaaac accaaatgct aagagttggg ctgaaaatga
      841 attagcgact atgggtaaaa tcgctttagc tgaaaaattt atgattggta tttttgtcat
      901 tgctctaaca ttatggattg tcggaagttt cattcatatt gatgcaactt taacggcctt
      961 tattgcgcta gcattgttat tattgacagg tgtcttaaca tggcaagaca ttttaaacga
     1021 aacaggtgct tggaacacat tagtatggtt ctcagtatta gtgttaatgg ccgaccaatt
     1081 aaacaagctt ggatttattc cttggttaag taaatccatt gctacaagtc ttggtggctt
     1141 aagctggcct atagtactgg tcattttaat attgttctac ttctattcac attacttatt
     1201 tgcaagttct acagcacata tcagtgcgat gtatgcagca ttactaggcg ttgccatcgc
     1261 agccggtgca ccaccattat tcagtgcatt aatgttaggt ttcttcggta acctattagc
     1321 ttcaacaaca cactatagta gtggtccggc gccgattcta ttctcttcag gttacgtgac
     1381 tcaaaaacgt tggtggacaa tgaacttgat cttaggtttc gtctacttta ttatctggat
     1441 tggtttagga tcactttgga tgaaagtaat tggtatattt taaaatattt aaattagtgc
     1501 tcgaatctca ttgatttggg cgctttttaa tttgtattta aaatcaacct ttgctaaatc
     1561 aagactccct ttttaaaata cgtttatcct ttaaatcatt gcgtgcttca ctgaaaattt
     1621 gtataaagat ttaagtcatt acgtaacatc acataaaata catttctata ctattccgtt
     1681 tcattgatca acattacgta tgccctcata aatcatgaaa taaaaaaaca ccttcgttta
     1741 aattcatttt aagtgcgaat tcaacgaaag tgatttattt catattcaat gttttaaatt
     1801 tatacagctg tcactgttac tgcacacata cctcagtctc accttatcat gacacaaagg
     1861 atattgaaaa cacgtataat tatctttcct cgtattttat agggtttttg cgaccggatg
     1921 tttcttcaat ttaatgtatt gagaaagact atataacaca atacctgtcc aaataaatat
     1981 aaacgtaatt aattgatcta tactaaaagg ctctttgaaa acaaatatgc cgagtacaaa
     2041 cattattgtt ggtccaacgt attgaataaa tcctattagc gaaagtggaa tacgttttgc
     2101 cccggctgag aataggatta gtggtattgc cgtaatagca ccagaaaata acaaccaaaa
     2161 tgatgacatg ttcaatccaa atgacatctg atgttgctgc cataaataaa taacgtatat
     2221 tagtccagca ggtgctgtaa caatacattc aatcgtaata ctgctgatgg catcaatatg
     2281 tactactttt ttcaataatc catatgtacc aaaggataac gctaatataa tagaaacgat
     2341 tgggaattct ccaatcttga gcgtcatata taatacaccg atgaatgcga ataaaatggc
     2401 tagccattca aatttattga atctttcttt taaaaagata agtgcgagca aaatgctaac
     2461 aagtggattt atataataac ctaaacttgt ttgtaggacg tgaccgttcg ttacagccca
     2521 aataaatgta ccccaattta atgtaatgac atagcctgct acgacaatcg ctaatagctg
     2581 aatgggcttg cctaacaatt gattcatatc tcgttgaaat gcattgcgtt gtttttgtcc
     2641 aaccgcgagt atgaatatca tgaatattgc tgaaaatata atacgaaagg ctaaaatttc
     2701 aaatgcgcct attgcatcaa cgaactgcca atatataggt agtattcccc acagaatgta
     2761 tgcactgagt gctaaaaata tacctttttt atattctata ttcacttaca tacctcctta
     2821 ctctcccaaa tttttaacat gttacgctca cttggttatg ctaatataac gattttacta
     2881 ataatatttc gataaagata tcattttgtt tatatttccc acatttattc accaaccact
     2941 aaacaatatt aattttataa ataattctgt acaaatcagg gtatattgcc agaaagacta
     3001 ccatacaaca taaaggatgg atacaaatga ctttacctaa aattggaaag cctgcaacac
     3061 gcgcgctaaa ttcacaaggt atatacacat tagaagcagt atcacaatat acgaagtcat
     3121 ctctaatgga gatgcatggc gttggtccta aagctatatc aatattggaa caagctttat
     3181 ttcagcacca actacatttt aaaacggaag ttcagtcatc attacctttt aagttaacag
     3241 gagatgtgtc atgcaatcat gcgcctaaac gtcaacaaat gatagatttt atagttgcta
     3301 cagcagcatt agatattgaa ttattacgtt cactggttac aacagaattt atttggtctg
     3361 tgcctggaca ctttgacatt tatggacctc aaattttaat tcaagaacta tctaactatt
     3421 acaatcaggt tgctagtctc aatatccact ccaacatcac acatgggtgc ttaggttcca
     3481 tgcatggtat tgaaatatta aaaactggta aagagattca ttttgctcat ttttttgaat
     3541 ttgaaaatca taaaaaggat gccaagttaa gcaaagtgac atcttacatt gttgttgctt
     3601 aatgactcat aatctataac gctaaaaact atcgtaatta catatatatg ttaataaaac
     3661 aaataatttt gcataatatt tttgttatat gaatgctttt tactagagtg cacattgaac
     3721 atacagtatg attaaatatc atgttatgaa agaaggatat attttgactg tttttaaaaa
     3781 tgagcgctta agctggttac catacatagc tattgtcatt ttgttacacg ttattgggtt
     3841 tagtttttta tggattgctg gaaaagacca tcatatctta tttggtatgg ggattcttgc
     3901 atacacatta ggtttgcgtc atgcatttga tgcagatcat atcgccgcga tagacaatac
     3961 cgttcgtaaa ttattacaac aacgcaaaga tccatctggt gtgggtttct atttttcaat
     4021 tggacattca tctgttgtat ttttaatggc tgttttttta gggatatctg taaaatgggc
     4081 taaagatgaa ttaccacatt tccaagatat tggtggaacg attggtacac tagtttcagg
     4141 tttcttttta gtgcttatcg gtgtgttgaa tctaattatt ttaatctctt taattaactt
     4201 gttcgctaaa ttacgtcgtg aacacattga agaagctgaa gtcgatgtat tacttgaatc
     4261 tagaggattt gtttctcgat ttgtaggacc ttatttcaag ttaatcacgc gtagttggca
     4321 cgtattgcca cttggctttt tatttggact tggttttgat acagctagtg aaattgcgtt
     4381 actcgctctt tcttcaggcg catcacaaca agccatttca tttatcggaa tcttatcttt
     4441 accaatttta ttcgcatcag gtatgagttt attggataca ttagatggtg ttgtaatgaa
     4501 gtatgcctat aattgggcat tcttcaatcc tattcgcaaa atctattaca atataacgat
     4561 tactgcgata tcagtcatgg cagcattagt gattgggatg attgaattgc tacaaattct
     4621 tgctgataag ctagatttac atggtgcgtt ttgggcattc attggttcga tcgaatttga
     4681 ttatttaggc tatattttag ttgcattatt tttaattact tggcttattt caagtttaat
     4741 ttggaagttt ggtcgcatag aacacaaatg gtctaaataa agtaacttgt cttgcaacta
     4801 cctttgtatt gttgcatttt tagacaccct gcacacttct tccattatgc tacgctctca
     4861 gtagtcatct ttaaatcgtt tcttttagag atgacttcat tttgagagcg tatttttcta
     4921 tttaaaatgt atttatccaa aattcaatgc tttcgaattt aaaatttcag catttctgtt
     4981 taaaatttaa ttataatctt taatacagga ggcgctatgc ttatgaatat tgcgaagtta
     5041 gagaattatt tacaaattga ttcatctcgt tataatcgac cgagtattga agcattaaat
     5101 tattatgcaa cacgttttat gttaactgta ccttttgaaa atattgatgt tcaaaatagt
     5161 aagccgatat ctataaatat cgatgcactt tttaataaaa ttgttcatga taaacgtggt
     5221 ggtttttgtt atgagttaaa tacatttttc aaagcatatt tacaacaaaa aggatttaat
     5281 cctgaattaa tgtcagctac tattcacaca cccggtggcg gtcgtagtct gaacggttca
     5341 catgcttcac ttgttgtttc tataaatgat gtattctatg taactgatgt tggctttgga
     5401 gacttacctc tacatgccat tcctattact tcatctgagc atacacaacc aattactgat
     5461 atcagtggta cattccgcgc tatttttaac aacgaagaca aggatatttt ctatgttcaa
     5521 aaattcgaaa atgatcattg gcatacaaaa tatgaagctg aattcaaacc taaacaaata
     5581 gaagatttta atagcaacat cgaatataat caaacgcatc cagattcaat atttgtacag
     5641 catttattaa ttacaatgcc acaatcattt ggccgtgcta caatgtctga aaatcattta
     5701 actttaacaa ggaatggtag ctcagaaaaa tttgatgtca ctaaagataa ctataaacat
     5761 tttttagaaa aatattttgg actaaatgta actattaatc gtattgaaaa acaataatag
     5821 taaaatataa aaatatgggc gtatcttact tattgtaggt agcgcccctt ttttatgctt
     5881 cataaacaca acaaaaagga tatgacacaa acttcatgtc acacccttct tttgaaccgc
     5941 ttcattttac ttaagcaagt gcatctacaa cataaatgac acctacgaat acaaatgcaa
     6001 tagcaatcat cgtcgaaata aagacgatac ccattaatgg attaaataat aaaatgacac
     6061 cgaaaacaat acctaataaa ttaaaaatca ctgaaattag ttttaagcta ccactacccg
     6121 taaacgtaaa taatcctgca atagaactaa aaatgaacca gaatgcaaac atgtagataa
     6181 aaaatgccga gctagcgcca acattaaaaa tgacaataag accaaagaga atatctacaa
     6241 tccccataaa cagaatccaa ttttggttac cacctactaa agcttttgct tttctacggt
     6301 aaatgatttg aatcacaccg ttaattaata caaacagtcc aatcaaccag gtaatagcat
     6361 aaaaattttc aattggaaat gtaaaaataa cgactgccaa cattaataat aatacaccca
     6421 ttattaaact agaccattta ataccttgat ttcctttttc cataacaatc aattcctttc
     6481 aaataaagcc ctcttttaat ttttacaaaa aatttattat acaataatta aattcccttt
     6541 tctcaattta cacaaacatc atcacttaga aagcaccatt gcttttgtct aatatgtacg
     6601 attaatctgt atcctcactc aaaaatctac taagtagcga tagctttcaa ctctgtaagg
     6661 ttcattcatt gaattgtaag tttagataaa ggctactact ccaaacatta tttaaaatga
     6721 aattaaatac aaaaggagtg agactatgac aatattatca gtgcaacatg tttcaaaaac
     6781 atacggtaaa aagcacacat ttcaagcact taaagatatt aactttgaca tacaaaaagg
     6841 cgaattcgtt gcgattatgg ggccttctgg atcaggtaag acaaccttat taaatgtact
     6901 aagttcaatt gaccaaattt ctagcggtag cgtgattgct aacggacaag agcttaataa
     6961 acttaatcaa aaagcacttg ccaaattccg taaagaatca ttaggtttca tcttccaaga
     7021 ttacagtatt ctgccgacat taactgtcaa agaaaacatt atgttacctt tatctgttca
     7081 aaaaatgtcg aaagcaacaa tggaagaaaa ttataaagcg attactacag cattaggtat
     7141 ttatgaccta ggaaataaat accctagcga attatctggt ggtcaacaac aaagaactgc
     7201 agcagcgaga gcatttgttc acaaaccaca aatcatattt gcagatgagc caacaggcgc
     7261 actcgactcg aaaagtgcaa atgacctatt acaacgtttg gaagaaatga ataaatcgtt
     7321 tgatacaact attgtcatgg ttacacatga tccggttgca gcaagttttg cagaacgtgt
     7381 catcatgcta aaagatggcc aaattcatac acaactttat caggaaggac gttctaaaca
     7441 ggccttttat gaagacattg tacatcttca atcagtatta ggtggtgtct caaatgacat
     7501 ttaaccatat cgttttcaaa aacttacgac aaaacttaaa acattatgca atgtatttat
     7561 tttcattatt ttttagcatc gtcttatatt tcagttttac aaccttacag tttactaaag
     7621 gtgtaaataa tgacgactcg atggccatca ttaagaaagg tgctttagtc ggatcaatat
     7681 ttttatttat cattattgtc atctttttaa tgtatgccaa tcatttattc gtgaaacgcc
     7741 gtacacgtga atttgcgcta tttcagttga ttggtttaac acgacaaaac attttgaaaa
     7801 tgttagcact tgaacaaatg attgtatttt taatcacagg tgttgtcggt gtattatgtg
     7861 gcattgcagg tgcacaatta ttgctttcca tagtatctaa attgatgtcg ttatcgatta
     7921 acttatcgat acatttcgaa cctatggccc ttgttttaac tattttcatg ctaattattg
     7981 cgtatgtact gattttattt caaagtgctt tatttctaaa aagacgtagt atcttatcaa
     8041 tgatgaaaga ttcaattaaa actgatgcca caactgctaa agtaacgact gcagaggtta
     8101 tttcaggcgt attaggtatt gctatgattg cactaggcta ttatatggcg acagaaatgt
     8161 ttggaacatt taaagcacta actatggcta tgacatcacc gtttatcatt ttatttttaa
     8221 cggtagtagg cgcctattta ttcttcagaa gttccgtgtc actaattttt aaaactttga
     8281 aaaaatctaa aaatggacgc gtatctatta cagatgttgt atttacatcg tctattatgt
     8341 accgaatgaa gaaaaatgcg atgtctttaa ctatcattgc aattatttct gcagttacag
     8401 taactgtatt gtgtttcgcg gcattatcta aatcaaatac agatcaaacc cttacatcta
     8461 tggcaccaaa tgaatttaac gtggtcgcta ctcaagatgc taaacaattt gaaactaaac
     8521 taagccaaca acaaattaca ttttctaaaa atgcttacga aactattact gtagataatg
     8581 ttaaagatca ggttattact ttggaaaatg gtagtgattc aggtcgcacg aattcaatat
     8641 taagtgcaaa taacaaggta acaggcaaca atgccatcat tacaaataca aaatcacttc
     8701 ctaacattat taatatccat ttaaacaaag atttagtagt aaaaggaact aagaatgaaa
     8761 ctttccgtgt tacacaagaa gacaaaggta gggtttatcc tttaaatcta agcttcaact
     8821 cacctgtcgt cgaagtaagt cctgaaaagt atcagcagtt gaaaacacaa aataacgttc
     8881 atacttttta tggatatgat attaaacaaa catcacaaaa ggaaaaagct caagcaattg
     8941 caaaacagtt tggagacaaa gtcatcactt atgatgaaat gaaaaaagag gtcgatgcta
     9001 ctaacggtat tctaatattt gttacatcat ttttaggatt agcattttta gtagcagctg
     9061 gatgtattat ttatattaaa caaatggatg aaactgagga tgaactaagt aacttcagaa
     9121 tattaaaacg catacagata tgcttaaggg attattatta aaaattactt tcaacttcgg
     9181 tttaccatta ttgattgcta tattacatgc agtattcgcc gcaatagcat ttatgaaatt
     9241 gatgggtaat atttccttca tgccggttat tgtagtaatt gttgtataca ccctcattta
     9301 cattactttt gctttaattg cttttgtaca ttcaaacaaa ttgattaaga aaaccattta
     9361 acacttacga ttaaaagatg cttcattgag attttgattt taagcaatgc agtataacta
     9421 ttaactgatt tataaaaagg agagattgaa tcatgaaatt tagagaatta gtaaaacaat
     9481 catacgagga tttgaaaaat ttgactgtaa cttggtataa cgtgattgct ttagcaatga
     9541 ttgttattgt cttaagcgta gttacaacac caattatagg tattccagct ggtttattag
     9601 gtggcggcta ttatttaaaa cgtcgtgaag aaaaaggtaa ataagcacat tccatcggca
     9661 tataacattg ctatgacctt tcaagtcaga ttttaatatc aacaagtagc atttatcgct
     9721 gcttgttttt tatgaattgc ttataaaatc gtcatgttta aacgttatgt cactttgaaa
     9781 actagcaaac accataaaag actacaattt tttattttta taaacccctt taaatcaacg
     9841 tttctaaggt ttttaaaaat ctttttaaat ttattttcaa aaaacactgt acattatgcc
     9901 aatatgagcg tatagttggt cttacgtaat aaaagctcgt gaattaaatt gtagtgtatt
     9961 tgtttagaat atcctctttt ttagttatga atttgttaca aatattaagt gcaaaagcac
    10021 atggaggttt tctatatgaa taacggtaca gtaaaatggt ttaacgcaga aaaaggtttt
    10081 ggtttcatcg aacaagaaaa tggcggagac gtattcgtac atttctcagg tatcgctagc
    10141 gatggctaca aaactttaga agaaggtcaa aaagttactt tcgaaatcac tgaaggtcaa
    10201 cgtggagacc aagcagttaa cgtacaaact gtttaatctt acaaaataaa acgactcatt
    10261 ataaatgaat cgctttgatt accaacaagg ttctaaatct tgttggtatt tttttatgct
    10321 attttggata atatgagaca atacgatgat gatttataaa aacttatttg ccatatacac
    10381 actaaggaaa attatgaggc tgagtatacc aattgctgat ataatagaaa gcgcatttgt
    10441 catcgtattt ctagctttag tcgtttgtac ttcactagga tcttttccat tcataaaagc
    10501 aacaattcga tcgtagttgt ccattttatg cacttttttt aaatcttcta ttttcataga
    10561 tgcataaaaa cctattccca aaatgattgc caccgttata ccccaagtcc aattccaata
    10621 aacaactaaa ggtcccatta aaattgcagc taatgtcatg aaaataagca tcacatatgt
    10681 ccataagtta agacttcgtt gcactttaat atcaggtaca aatggaatag tcccttttac
    10741 taaatcatct aaagttacat tgaacaattc gcacatcatt aataagttat gtatgtctgg
    10801 taagcttttg tcattttccc aattagaaat actctgccta gatacatata acttttcagc
    10861 aagatattct tgtgaataac catctcgttc cctatacttt ttaatttgtt tactcagatt
    10921 caattacata cgcctccagt caattagtac aatcattaca atcagactat cagataaact
    10981 tagttaaagt cacctttatg attgactaaa tgttttgaca tacgacaaca tcggggttcg
    11041 aagactccct taagacagac ctgtataatt acgcgcttgt aattttctca atgccaactt
    11101 gaatttcact tattaatgat gcaacttttc catgctattc aatgcttgca aagttagtcc
    11161 tagttaagag attacgttca cactatttac tattcgattt tccagctttc caactatgct
    11221 tttctaatct tttttgtgct ataaattcta ccgcttcaaa aatacatact gcactgatga
    11281 accagcttag gaaaccaaca ataatgctaa tcaaccacat gtgcgtcata tgtaacatca
    11341 tccatgtaac taaaatacaa actaataccc ctgtcatacc aaacactgcg ccacgactta
    11401 catgcacagc gactttatca ccatattgca tacctgtaat aaacatagac actaaaaata
    11461 ctgccggaaa cgttgcaaat ataccgccaa attctttcca aggtagtgtt acagatacaa
    11521 tataacttaa taatactgca aatcccccca caaaaaattt cattaatgtt aatttcatga
    11581 tgttctgcct ccaaaaattt attacttaat aactgaatac aactaattat cacgcatttt
    11641 ccaaactata aatgtctaat actgaatatt gcgagggaaa taacaaacca acaaacaact
    11701 gtgaatattg cgcctttccg ataaccttta tgcttaatat agagagatgt caaaaataca
    11761 gttaatatac aagacagaat accgacaatt gctccagtac taagattcat cgacatctcc
    11821 actaattgcg taccacgatg atctaaagct aatgcgataa tagccgcaag aaagactgcc
    11881 ggcatagtag ctataatacc tcctaactta ccacctacct tatcagcaat aattgaagct
    11941 aatgcaacag cgataccacc aatgacaaaa tgaagaattg cacttccaat tgaaaacatc
    12001 ttcaacgtac ctttctataa ttaattcaat acaaaacgta tcattcacac aaagtgtaaa
    12061 acttaaattt tctactgtaa aggcaactca agcgatgttc ataaagcttt catgatactt
    12121 tcataaacta gcaataaagg gttcaaatga aggtatatcc agctcaaatt agggagtggg
    12181 acagaaatga tattttcgca aaatttattt cgtcgtccca ccccagcaag gttgactaga
    12241 attgaaaaaa gcttgttaca agcgcatttt cgttcagtca actactgcca atataacttc
    12301 gtagagcata gaacattgat ttatgtacca gcctgatcaa catataaata taaattttta
    12361 tgtttcacgt aaaacaaaaa aagacaaagc tgttatgatc ttagctttgt cttttatgtg
    12421 ttagtcatca tgtatacgac atcctactac taacgtttat atattcgaat ttttatttca
    12481 taataatctt catgatcttt gtctttatgc tcaacatgta gtcctgtttg ttgaatcgct
    12541 tggatacttt tgcctacctc atctcgtgct tgagtgacat cttgtgcaaa gcgaaggttt
    12601 tgtgctttga ctttttcggg ccccgttttt tggcgtactc tatcttcagt ctgtttcaca
    12661 tttagttttt gtgcaatgac ttgctcaatc aacgcttctt gttcactatc agacaatgat
    12721 aataccgctc tcgcatggcg ttcagtaatt ttaccttctc ttaagcgaag tagtactttc
    12781 ggcgccaact tcaataaacg caacttattt gcaataaagc tttgactttt acctaaactt
    12841 tttgccaatt cactttgcgt tgtatcacca atttccaata atttcttata ggcttccgct
    12901 tcttcaacaa cagacaaatt ttctctttga atattctcaa ttaatgcaac aacagccgtc
    12961 tcttcatcat ccatatcacg aataataacg tctgcttgag gtaaatttag tgattgtatt
    13021 gctctaaatc ggcgctctcc agcaataatt tcaaacatat cttcttcaat cggtcttaca
    13081 acaataggtt gtagtaaacc atgttcatgt attgattcag caagttcttt aattttattt
    13141 ggttcaaaca cctgtcttgg ttgataacgg ttgggaacga tacgttcaat ttgaatggat
    13201 tcaacattac tattgcgatc ttcttcaata tgtccaatga tgtcatcttt gtttttcaaa
    13261 ccaaataatt ttgaaaaagg ttttttcatt atccattcgc tccttttatg ttagttgtaa
    13321 aggattatgc attatttttc aagtaaagga gtcttattag gcgtccctgg ttttcttggg
    13381 tactttttcg gcgtctgtct ttttttatca ataatgaaca tctggcgctc tccagcatct
    13441 tctggcaatt taaaggtatg tgtttctgta acgttaccac ctaacacact aattgcaaat
    13501 tttgcttctt ctaattcttc ttcacctttt gaagatttta atgcaacaaa ctgtccacct
    13561 tttttaatta gcggtaaaca caattcactc aacacggata atctagctac tgctcttgca
    13621 gtaacaacat cataagactc cctgtagaca cccttaccaa atgtttctgc tctatcgtgt
    13681 ataaagctga catcctgtaa ttgtaattct gacgctaaat ggtttaaaaa ttgaatacgc
    13741 ttatttaatg aatcaacaat cgtcactttt aactgcggaa acattatttt taacggaata
    13801 cttggaaaac cagctccagc gcctacatca catatactta taggctgatt aaaatcaaaa
    13861 taaaaactag gtgcaatgga atcataaaaa tgtttcaaat atacatcgtg ttcatctgta
    13921 atacttgtca aattcatctt ttcattccat tcaacaagta aacgataata tgtttgaaac
    13981 tgttgttttt gagtctcagt taattcaata ttatgttctt ttaattgttc tgctaaccat
    14041 tctacagtca ttagtcactc accctttgga gtttaccttg ttctaagtaa atcaataata
    14101 tagatatgtc tgctggattt acccctgata ttctagaagc ttgtgcaata tttaaaggtt
    14161 ttacttctga taatttttct cgcgcttcag tcgccaaact atcaatctta ctataatcta
    14221 agtcttctgg aattttcttc tcttccatac gcttaacttt ctcaacttgt tgtagtgatt
    14281 tattgatata accttcatat tttgtttgta tttctacttg ttcttcaaca tctgcattca
    14341 attgatgttc ttcttctaaa atttctaaaa ttatatcgta agtcatttca ggtctgcgta
    14401 ataaatcgat agctaaaata ccatctttta agcgagaacc accatgttgt tcgataatcg
    14461 cttgcgtatg ttcgtttggt ttaatacgaa tatctgataa acgcttaatt tccgcatcaa
    14521 tttgctgacg tttttcatta aaacgtgcat atcgttcttc agaaatcata ccaagttcat
    14581 atcccatatc cgtcaatctc aaatcagcat tatcatgacg tagtaacaaa cgatattctg
    14641 cacgtgatgt tagtaaacga taaggttcat tagtaccttt agttacaaga tcatcgatta
    14701 agacaccaat atatgcatct gaacgactta atatcttttc gcctgtgttt aacactttac
    14761 ctgcagcgtt aatacctgcc atcaatcctt gtcctgctgc ttcttcataa ccagatgtac
    14821 cattaatttg acctgcagta tataagtttt taatcatttt cgtttcaagt gtaggccata
    14881 actgcgttgg cacaatcgca tcatattcaa ttgcgtagcc ggcacgcatc atatctgctt
    14941 tttcaagacc tggtatcgtc tctaacattt gacgttgcac atgttcagga agacttgtag
    15001 acaatccttg cacatatact tcatttgtat tacgaccttc aggctctaag aaaagttgat
    15061 gtcgcggctt atcattaaat cgaacaaatt tatcttcaat tgaagggcaa taacgtggcc
    15121 cggttccttt aatcatccct gaatacattg cagatagatg taaattatca tcgataactt
    15181 tgtgtgtttc agcattagta tacgttagcc aacatggcaa ttgatctaat atatattctg
    15241 ttgtttcaaa gctgaatgca cgacctacat cgtcacctgg ttgtatttca gtcttcgaat
    15301 agtcaattgt ttttgaattt acacgcggtg gtgtacctgt tttaaaacga acaatatcaa
    15361 aaccaagttc tcttaaattg tctgataatg tgattgatgg taattggtga tttggtccac
    15421 ttgaatactt catattacct aaaatgattt caccacgtaa aaatgttccc gttgtaataa
    15481 ttactgcttt agataaatac tctgtaccaa tatttgtacg tacaccttta acttcattat
    15541 cttctataat aagttcgtct accatacctt gcattatatg caaattttct tcatcttcaa
    15601 tcacgcgttt catttcttgt tgataaagta ctttatctgc ttgcgctctt agtgctctta
    15661 cagcaggtcc tttacctgta tttaacattc tcatttgaat gtgtgtttta tcgattgttt
    15721 ttgccatttg tccacctaaa gcatcaattt cacgaacaac gatgccttta gctggtccac
    15781 ctacagatgg gttacatggc ataaatgcaa tattatctaa atttattgtt agcattaatg
    15841 ttttagcacc acgtcttgca gatgctaaac ctgcttctac acctgcatgt cccgcaccta
    15901 taacgattac atcatattct tgaaccacaa tataaacctc cttatttgat atcttactag
    15961 ccttcttaag acgatattcc gtctatttca attactattt acctaagcag aattgactga
    16021 ataactgatc gatgagttca tcacttgcag tctcaccaat aatttctcct aatatttccc
    16081 aagttctagt taaatcaatt tgtaccatat ccataggcac accagattct gctgcatcaa
    16141 tcgcatcttg tatcgtttgt cttgcttgtt ttaataatga aatatgtctt gaattagaaa
    16201 cataagtcat atcttgattt tgtacttctc caccaaagaa caaatctcga atttgtattt
    16261 ctaattcatc aataccttct tgttttaaca ttgaagtttg aattaatggc gtatcaccta
    16321 tcatatcttt aacttcatta atatctatgt tttgctctaa atccatttta ttaacaatta
    16381 cgattacatc ttcattttta accacttcat ataatgtgta atcttcttga gtcaatgctt
    16441 cgttattgtt taatacaaat aaaattaagt ctgcttggct aagagccttt ctagagcgtt
    16501 caacaccaat cttctctact atatcttctg tctcgcgtat accagcagta tcaactaatc
    16561 ttaatggcac gccacgaaca ttgacgtact cttctaagac atctcttgta gtacctgcta
    16621 cctcagttac aatcgcttta ttatcttgta ttaaattatt taacatcgat gatttaccta
    16681 cgtttggttt accaacaata actgtagata aaccttcacg cataattttg ccctgcgcac
    16741 cggtatctaa taaacgatta atttcctgtt tgatttcttt agactgctct aaaagaaatt
    16801 cagtagtcgc atcttcaaca tcatcgtatt caggataatc aatattcact tccacttgag
    16861 cgagtatctc taatatagat tgacgttgtt ttttgattaa gtcacttaga cgaccttcaa
    16921 tttgattcat cgcaacttta gaagctctat ctgtcttcga gcgaataaag tccataactg
    16981 cttcagcttg agataaatca atacgaccat ttaaaaaggc acgttttgta aattcacctg
    17041 gctcagccat tctagcgcca tatgtcatag taagttccag cactctatta atcgttaaaa
    17101 taccaccatg acaattaatt tctataatat cttcgcgtgt aaatgttttt ggcgctctta
    17161 acacagacac cataacttct tcaaccactt ctttagactc tggatcaata atatgaccgt
    17221 aattaatcgt atgtgatgga acatcattta aaagatgttt tcctttatat aatttgtcag
    17281 caatttcaac ggcttgcggt ccagacaatc gaacaattcc aattgcccct tcacccattg
    17341 gtgttgaaat actcgtaatt gtatctaaat ccatattgct actcgcctcc ttcaacgatg
    17401 tgaatacatt ttaaagtaag ttattataac cctaaggtca gtcttaaccc taaggtcagt
    17461 cttacgtttg tctgaggtaa gacttcggga tgcgttgagt ggttaatgtt ttccttcccc
    17521 taccctatcc ttacttaatc tttttattaa aaactttggc aattttaagt acgtgctcaa
    17581 gactattctg tatttgtaaa gtcgtcatat ctttagctgg ctgtcttgct attacaataa
    17641 tatctttggc caatatatgc gacttatgta ctttgaaatt ttcacgtatt gctcttttaa
    17701 tcttgtttct taacactgca ttacctagtt ttttagaaac actaatacct aagcgaaaat
    17761 ggtctatttc tttattatta caagtgtata caacaaattg tctgttggct acagaatgac
    17821 cttttttata tattctctga aaatctgcat tctttttaat tcggtaagct ttttccaata
    17881 acatcactcg cttatttatc gtttttattt gaagctatat ttaaacttct attgagctta
    17941 taacataaat ttctatttat tcttaattta aacgaaaaaa aagatcactg atgagtcagt
    18001 gatcttatgc agataaaact ttacggcctt tacgacgacg gcgcgctaaa actttacggc
    18061 cattttttgt gctcatgcgt tttctgaaac catgaacttt actatgttta cgtttatttg
    18121 gttgataagt acgttttacc atgcaaaaca cctccatctt tatacagtta tctaatatac
    18181 cagattatgc agactctgat cactttaata gataagattt tgcgtttctt agattaaaat
    18241 tctcgtatta gctcattgat tatctagtca taattcaagc aactactaca atataacaaa
    18301 atccttttta taacgcaagt tcattttata ctactgctca atttttttac ttttatcgat
    18361 taaagataga aatacacgat gcgagcaatc aaatttcata acatcaccat gagtttggtc
    18421 cgaagcatga gtgtttacaa tgtttgaata ccttatacag ttcttataca tactttataa
    18481 attatttccc aagctgtttt gatacactca ctaacagata ttctatagaa ggaaaagtta
    18541 tccacttatg cacatttata gttttcagaa ttgtggataa ttagaaatta cacacaaagt
    18601 tatactattt ttagcaacat attcacaggt atttgacata tagagaactg aaaaagtata
    18661 attgtgtgga taagtcgtcc aactcatgat tttataagga tttatttatt gatatttaca
    18721 taaaaatact gtgcataact aataagcagg ataaagttat ccaccgattg ttattaactt
    18781 gtggataatt attaacatgg tgtgtttaga agttatccac ggctgttatt tttgtgtata
    18841 acttaaaaat ttaagaaaga tggagtaaat ttatgtcgga aaaagaaatt tgggaaaaag
    18901 tgcttgaaat tgctcaagaa aaattatcag ctgtaagtta ctcaactttc ctaaaagata
    18961 ctgagcttta cacgattaaa gatggtgaag ctatcgtatt atcgagtatt ccttttaatg
    19021 caaattggtt aaatcaacaa tatgctgaaa ttatccaagc aatcttattt gatgttgtag
    19081 gctatgaagt taaacctcac tttattacta ctgaagaatt agcaaattat agtaataatg
    19141 aaactgctac tccaaaagaa acaacaaaac cttctactga aacaactgag gataatcatg
    19201 tgcttggtag agagcaattc aatgcccata acacatttga cacttttgta atcggacctg
    19261 gtaaccgctt tccacatgca gcaagtttag ctgtagccga agcaccagcc aaagcgtaca
    19321 atccattatt tatctatgga ggggttggtt taggaaaaac ccatttaatg catgccattg
    19381 gtcatcatgt tttagataat aatccagatg ccaaagtgat ttacacatca agtgaaaaat
    19441 tcacaaatga atttattaaa tcaattcgtg ataacgaagg tgaagctttc agagaaagat
    19501 atcgtaatat cgacgtctta ttaatcgatg atattcagtt catacaaaat aaagtacaaa
    19561 cacaagaaga atttttctat acttttaatg aattgcatca gaataacaag caaatagtta
    19621 tttcgagtga tcgaccacca aaggaaattg cacaattaga agaccgatta cgttcacgct
    19681 ttgaatgggg gctaattgtt gatattacgc caccagatta tgaaactcga atggcaattt
    19741 tgcagaagaa aattgaagaa gaaaaattag atattccacc agaagcttta aattatatag
    19801 caaatcaaat tcaatctaat attcgtgaat tagaaggtgc attaacacgt ttacttgcat
    19861 attcacaatt attaggaaaa ccaattacaa ctgaattaac tgctgaagct ttaaaagata
    19921 tcattcaagc accaaaatct aaaaagatta ccatccaaga tattcaaaaa attgtaggcc
    19981 agtactataa tgttaggatt gaagatttca gtgcaaaaaa acgtacaaag tcaattgcat
    20041 atccgcgtca aatagctatg tacttgtcta gagagcttac agatttctca ttacctaaaa
    20101 ttggtgaaga atttggtggg cgtgatcata cgaccgtcat tcatgctcat gaaaaaatat
    20161 ctaaagattt aaaagaagat cctattttta aacaagaagt agagaatctt gaaaaagaaa
    20221 taagaaatgt ataagtagga aactttggga aatgtaatct gttatataac agcactaata
    20281 ataactgtca ttttttacat ttctatatac taatgtgaca agatgagcaa aactcatttt
    20341 gtggataatg tttaaaagtc atacacacca tacacaagtt atcaacatgt gtataacttc
    20401 gccaaatcta tgtttttacg acttatccac caatccacag cacctactac tattactaag
    20461 aacttaaaac ctatataatt atatataaac gactggaagg agttttaatt aatgatggaa
    20521 ttcactatta aaagagatta ttttattaca caattaaatg acacattaaa agctatttca
    20581 ccaagaacaa cattacctat attaactggt atcaaaatcg atgcgaaaga acatgaagtt
    20641 atactaactg gttcagactc tgaaatttca atagaaatca ctattcctaa aactgtagat
    20701 ggcgaagata ttgtcaatat ttcagaaaca ggctcagtag tacttcctgg acgattcttt
    20761 gttgatatta taaaaaaatt acctggtaaa gatgttaaat tatctacaaa tgaacaattc
    20821 cagacattaa ttacatcagg tcattctgaa tttaatttaa gtggcttaga tccagatcaa
    20881 tatcctttat tacctcaagt ttctagagat gacgcaattc aattgtcggt aaaagtgctt
    20941 aaaaacgtga ttgcacaaac gaattttgca gtgtccacct cagaaacacg cccagtacta
    21001 actggtgtga actggcttat acaagaaaat gaattaatat gcacagcgac tgactcacac
    21061 cgcttggctg taagaaagtt gcagttagaa gatgtttctg aaaacaaaaa tgtcatcatt
    21121 ccaggtaagg ctttagctga attaaataaa attatgtctg acaatgaaga agacattgat
    21181 atcttctttg cttcaaacca agttttattt aaagttggaa atgtgaactt tatttctcga
    21241 ttattagaag gacattatcc tgatacaaca cgtttattcc ctgaaaacta tgaaattaaa
    21301 ttaagtatag acaatgggga gttttatcat gcgattgatc gtgcctcttt attagcacgt
    21361 gaaggtggta ataacgttat taaattaagt acaggtgatg acgttgttga attatcttct
    21421 acatcaccag aaattggtac tgtaaaagaa gaagttgatg caaacgatgt tgaaggtggt
    21481 agcctgaaaa tttcattcaa ctctaaatat atgatggatg ctttaaaagc aatcgataat
    21541 gatgaggttg aagttgaatt cttcggtaca atgaaaccat ttattctaaa accaaaaggt
    21601 gacgactcgg taacgcaatt aattttacca atcagaactt actaaaaata aatataaata
    21661 aaggatgacg tgattaatta aaacgtcatc ctttattttt tggcaaaaat aattctagat
    21721 gcgtatgtaa aataaatttg acagcatttt aaacagcaaa taaaagacgc caattaaatt
    21781 tatgacaaat gtatccaaaa tttaataagt gtgcttatat gccctttaaa tttaaaattt
    21841 taatagtcaa taacaagttg aatattaaag ttaaacgccg ttaaatagcg ttaaaaaatt
    21901 gaaaatgaca gtattgccaa aaaataagaa ttaattattt atatgtaaac ggtttctacc
    21961 tctattttaa atgaaatttg tgacaaaaaa aggtataata tattaatgac gcacaaagaa
    22021 atggagtgat tattttggtt caagaagttg tagtagaagg agacattaat ttaggtcaat
    22081 ttctaaaaac agaagggatt attgaatctg gtggtcaagc aaaatggttc ttgcaagacg
    22141 ttgaagtatt aattaatgga gtgcgtgaaa cacgtcgcgg taaaaagtta gaacatcaag
    22201 atcgtataga tatcccagaa ttacctgaag atgctggttc tttcttaatc attcatcaag
    22261 gtgaacaatg aagttaaata cactccaatt agaaaattat cgtaactatg atgaggttac
    22321 gttgaaatgt catcctgacg tgaatatcct cattggagaa aatgcacaag gaaagacaaa
    22381 tttacttgaa tcaatttata ccttagcttt agcaaaaagt catagaacga gtaatgataa
    22441 ggaactcata cgttttaatg ctgattatgc taaaatagaa ggtgagctta gttatagaca
    22501 cggcacgatg ccattaacaa tgtttataac taaaaaaggt aaacaagtca aagtgaatca
    22561 cttagagcaa agtcgtctaa ctcaatatat tggacacctc aatgtggttc tatttgcgcc
    22621 agaagatttg aatattgtaa aaggctctcc tcaaataaga cgacgcttta tagatatgga
    22681 gttgggccaa atttctgctg tttacttaaa tgatttagct caataccaac gtattttaaa
    22741 gcaaaagaat aattacttaa agcagttaca attaggccaa aaaaaggact taacaatgtt
    22801 ggaagtatta aatcagcagt ttgctgaata tgcaatgaaa gtaactgata aacgtgcaca
    22861 ttttattcaa gagctagagt cgttagctaa accgattcat gctggtatca caaatgataa
    22921 agaagcgttg tcgctaaatt atttacctag tcttaaattt gattatgctc aaaatgaagc
    22981 ggcacgactt gaagaaatta tgtctattct tagcgataat atgcaaagag aaaaagaacg
    23041 aggcattagc ttattcggac cacatcgaga tgatataagt tttgatgtga atggcatgga
    23101 tgctcaaaca tatggttctc aaggacagca acgtacaacg gctttgtcca ttaaattagc
    23161 tgaaattgag ttaatgaata tcgaagttgg ggaatatccc atcttattat tagacgatgt
    23221 actcagtgaa ttagatgatt cgcgtcaaac gcatttatta agtacgattc agcataaagt
    23281 acaaacattt gtcactacga catctgtaga tggtattgat catgaaatca tgaataacgc
    23341 taaattgtat cgtattaatc aaggtgaaat tataaagtaa cagaaagcga tggtgactgc
    23401 attgtcagat gtaaacaaca cggataatta tggtgctggg caaatacaag tattagaagg
    23461 tttagaagca gtacgtaaaa gaccaggtat gtatatagga tcgacttcag agagaggttt
    23521 gcaccattta gtgtgggaaa ttgtcgataa tagtatcgat gaagcattag ctggttatgc
    23581 aaataaaatt gaagttgtta ttgaaaaaga taactggatt aaagtaacgg ataacggacg
    23641 tggtatccca gttgatattc aagaaaaaat gggacgtcca gctgtcgaag ttattttaac
    23701 tgttttacat gctggtggta aattcggcgg tggcggatac aaagtatctg gtggtttaca
    23761 tggtgttggt tcatcagttg taaacgcatt gtcacaagac ttagaagtat atgtacacag
    23821 aaatgagact atatatcatc aagcatataa aaaaggtgta cctcaatttg acttaaaaga
    23881 agttggcaca actgataaga caggtactgt cattcgtttt aaagcagatg gagaaatctt
    23941 cacagagaca actgtataca actatgaaac attacagcag cgtattagag agcttgcttt
    24001 cttaaacaaa ggaattcaaa tcacattaag agatgaacgt gatgaagaaa acgttagaga
    24061 agactcctat cactatgagg gcggtattaa atcgtacgtt gagttattga acgaaaataa
    24121 agaacctatt catgatgagc caatttatat tcatcaatct aaagatgata ttgaagtaga
    24181 aattgcgatt caatataact caggatatgc cacaaatctt ttaacttacg caaataacat
    24241 tcatacgtat gaaggtggta cgcatgaaga cggattcaaa cgtgcattaa cgcgtgtctt
    24301 aaatagttat ggtttaagta gcaagattat gaaagaagaa aaagatagac tttctggtga
    24361 agatacacgt gaaggtatga cagcaattat atctatcaaa catggtgatc ctcaattcga
    24421 aggtcaaacg aagacaaaat taggtaattc tgaagtgcgt caagttgtag ataaattatt
    24481 ctcagagcac tttgaacgat ttttatatga aaatccacaa gtcgcacgta cagtggttga
    24541 aaaaggtatt atggcggcac gtgcacgtgt tgctgcgaaa aaagcgcgtg aagtaacacg
    24601 tcgtaaatca gcgttagatg tagcaagtct tccaggtaaa ttagccgatt gctctagtaa
    24661 aagtcctgaa gaatgtgaga ttttcttagt cgaaggggac tctgccgggg ggtctacaaa
    24721 atctggtcgt gactctagaa cgcaggcgat tttaccatta cgaggtaaga tattaaatgt
    24781 tgaaaaagca cgattagata gaattttgaa taacaatgaa attcgtcaaa tgatcacagc
    24841 atttggtaca ggaatcggtg gcgactttga tctagcgaaa gcaagatatc acaaaatcgt
    24901 cattatgact gatgccgatg tggatggagc gcatattaga acattgttat taacattctt
    24961 ctatcgattt atgagaccgt taattgaagc aggctatgtg tatattgcac agccaccgtt
    25021 gtataaactg acacaaggta aacaaaagta ttatgtatac aatgataggg aacttgataa
    25081 acttaaatct gaattgaatc caacaccaaa atggtctatt gcacgataca aaggtcttgg
    25141 agaaatgaat gcagatcaat tatgggaaac aacaatgaac cctgagcacc gcgctctttt
    25201 acaagtaaaa cttgaagatg cgattgaagc ggaccaaaca tttgaaatgt taatgggtga
    25261 cgttgtagaa aaccgtagac aatttataga agataatgca gtttatgcaa acttagactt
    25321 ctaagcgctg tgaactgaac ttttgaagga ggaactcttg atggctgaat tacctcaatc
    25381 aagaataaat gaacgaaata ttaccagtga aatgcgtgaa tcatttttag attatgcgat
    25441 gagtgttatc gttgctcgtg cattgccaga tgttcgtgac ggtttaaaac cagtacatcg
    25501 tcgtatacta tatggattaa atgaacaagg tatgacaccg gataaatcat ataaaaaatc
    25561 agcacgtatc gttggtgacg taatgggtaa atatcaccct catggtgact tatctattta
    25621 tggagcaatg gtacgtatgg ctcaagattt cagttatcgt tatccgcttg ttgatggcca
    25681 aggtaacttt ggttcaatgg atggagatgg cgcagcagca atgcgttata ctgaagcgcg
    25741 tatgactaaa atcacacttg aactgttacg tgatattaat aaagatacaa tagattttat
    25801 cgataactat gatggtaatg aaagagagcc gtcagtctta cctgctcgat tccctaactt
    25861 gttagccaat ggagcatcag gtatagcggt aggtatggca acgaatattc caccacataa
    25921 cttaacagaa ttaatcaatg gtgtacttag cttaagtaag aaccctgata tttcaattgc
    25981 tgagttaatg gaggatattg aaggtcctga tttcccaact gctggactta ttttaggtaa
    26041 gagtggtatt agacgtgcat atgaaacagg tcgtggttca attcaaatgc gttctcgtgc
    26101 agttattgaa gaacgtggag gcggacgtca acgtattgtt gtcactgaaa ttcctttcca
    26161 agtgaataag gctcgtatga ttgaaaaaat tgcagagctc gttcgtgaca agaaaattga
    26221 cggtatcact gatttacgtg atgaaacaag tttacgtact ggtgtgcgtg tcgttattga
    26281 tgtgcgtaag gatgcaaatg ctagtgtcat tttaaataac ttatacaaac aaacacctct
    26341 tcaaacatca tttggtgtga atatgattgc acttgtaaat ggtagaccga agcttattaa
    26401 tttaaaagaa gcgttggtac attatttaga gcatcaaaag acagttgtta gaagacgtac
    26461 gcaatacaac ttacgtaaag ctaaagatcg tgcccacatt ttagaaggat tacgtatcgc
    26521 acttgaccat atcgatgaaa ttatttcaac gattcgtgag tcagatacag ataaagttgc
    26581 aatggaaagc ttgcaacaac gcttcaaact ttctgaaaaa caagctcaag ctattttaga
    26641 catgcgttta agacgtctaa caggtttaga gagagacaaa attgaagctg aatataatga
    26701 gttattaaat tatattagtg aattagaaac aatcttagct gatgaagaag tattactaca
    26761 attagttaga gatgaattaa cagaaattcg agatcgtttc ggtgatgatc gtcgtactga
    26821 aatccaatta ggtggatttg aagatttaga agatgaagat ctcattccag aagaacaaat
    26881 tgtaattaca ctaagccata ataactacat taaacgtttg ccggtatcta catatcgtgc
    26941 tcaaaaccgt ggtggtcgtg gtgttcaagg tatgaataca ttggaagaag attttgtcag
    27001 tcaattggta actttaagta cacatgacca tgtattgttc tttactaaca aaggtcgtgt
    27061 atacaaactt aaaggttatg aagtgcctga gttatcaaga cagtctaaag gtattcctgt
    27121 agtgaatgct attgaacttg aaaatgatga agtcattagt acaatgattg ctgttaaaga
    27181 ccttgaaagt gaagacaact tcttagtgtt tgcaactaaa cgtggtgtcg ttaaacgttc
    27241 agcattaagt aacttctcaa gaataaatag aaatggtaag attgcgattt cgttcagaga
    27301 agatgatgag ttaattgcag ttcgcttaac aagtggtcaa gaagatatct tgattggtac
    27361 atcacatgca tcattaattc gattccctga atcaacatta cgtcctttag gccgtacagc
    27421 aacgggtgtg aaaggtatta cacttcgtga aggtgacgaa gttgtagggc ttgatgtagc
    27481 tcatgcaaac agtgttgatg aagtattagt agttactgaa aatggttatg gtaaacgtac
    27541 gccagttaat gactatcgtt tatcaaatcg tggtggtaaa ggtattaaaa cagctacgat
    27601 tactgagcgt aatggtaatg ttgtatgtat cactacagta actggtgaag aagatttaat
    27661 gattgttact aatgcaggtg tcattattcg actagatgtt gcagatattt ctcaaaatgg
    27721 tcgtgcagca caaggtgttc gcttaattcg cttaggtgat gatcaatttg tttcaacggt
    27781 tgctaaagta aaagaagatg cagaagatga aacgaatgaa gatgagcaat ctacttcaac
    27841 tgtatctgaa gatggtactg aacaacaacg tgaagcggtt gtaaatgatg aaacaccagg
    27901 aaatgcaatt catactgaag tgattgattc agaagaaaat gatgaagatg gacgtattga
    27961 agtaagacaa gatttcatgg atcgtgttga agaagatata caacaatcat cagatgaaga
    28021 tgaagaataa taaaaaaata agacttccct atatgtaggg gagtcttatt tttatgctag
    28081 aaagtaatgc tgtactatat tcaatgatta gtaatgatta actttctaat tgtttcattg
    28141 cgtaaggtat ttcattgata agtcttgatg gtggtaccac atacatatct tttgcaaggt
    28201 tttcgccaat aaaactatgt gtatatgtgg cactcataac cgcttctttt aagttatcaa
    28261 attgaccgac aaaacttgta atcataccag caagtgtatc gcccatacca ccagtcgcca
    28321 ttgctgggct accgattgtt aatttaaagt cttcatcttt aaagaaaatt tcagtaccat
    28381 gttttttaag tacaacagtt gcacctaaac gatcaactgc ttcacgatta cgctcatatg
    28441 tctgttcctc aataggaata ccacttaatc gttcccattc tttgaggtgt ggagtaaaga
    28501 tcacacgaca tgtaggtaat tgcggtttca gtttactaaa gattgtaatc gcatcgccgt
    28561 ctacgattaa attttgatgc ggttgtatat tttgtagtag gaatgtaatg gcattatttc
    28621 ctttgaaatc aacgccaaga cctggaccaa ttagtatact gtcagtcatt tcaatcattt
    28681 tcgtcaacat tttcgtatca ttaatatcaa taaccatcgc ttctgggcaa cgagaatgta
    28741 atgctgaatg atttgttgga tgtgtagcta cagtgattaa accactaccg ctaaatacac
    28801 atgcacgagc cgctaacata atggcaccac ctaagttagc agatccacca attaataaaa
    28861 ttttgccata atcaccttta tgtgaatctt ctttacgctt aggaatgtta atagaattta
    28921 acgtttccat agtgatataa cctcccatgt aaaagccttt tccaaattta ttcaatttta
    28981 aaaatatata gtaactttta acaaaatgta ttataaattt ctgaattcat tattatttgt
    29041 cgttaaatac aatagaaaat actatacctg tatatgcaat tcgtcaatag ataaattatt
    29101 aaatatgctt acaacaatct taatatcctt taacgcacta caatagtgct ctgataatag
    29161 gttataaatg tacgtaaaac cattgtttca ataaaaatga aaacgtatac ttcaagaagg
    29221 atgggttact taatataaac aagggggtaa catatatgac tttatattta gatggtgaaa
    29281 cactaacaat tgaggatatt aaatcatttt tacaacaaca atcaaagatt gaaattattg
    29341 atgatgcgtt agaacgtgtc aaaaaaagta gagcggtagt tgaacgtatt attgaaaatg
    29401 aggaaacggt ttacggtatc actacaggtt ttgggttatt tagtgatgta cgtatagacc
    29461 cgacgcaata taatgaatta caagtgaatc tgatacgctc acatgcctgt ggactaggtg
    29521 agccattttc aaaagaagta gcattagtca tgatgatttt acgattgaat acattattaa
    29581 aaggtcattc aggtgccact ttagaattag tgagacaatt acaatttttt ataaatgaac
    29641 gtattatacc gataatccca caacaaggct ctctcggtgc atcaggagat ttagcgccat
    29701 tatcacattt agcattagca ttaattggtg aagggaaagt attgtacaga ggggaagaaa
    29761 aggatagtga cgatgtatta agagaattaa atagacaacc tttgaacctt caggctaaag
    29821 agggtttagc attgattaat ggtacgcaag ctatgacagc tcaaggtgtc attagttata
    29881 tagaagcaga agatttaggt taccaatctg aatggattgc tgcattaacg catcagtctc
    29941 ttaatggcat tatagatgca tatcgacatg atgtgcactc tgttcgtaat tttcaagaac
    30001 agattaatgt ggcagcgcgt atgcgtgatt ggttagaagg atcaacatta acgacgcgac
    30061 aagcagaaat acgtgtacaa gatgcatata cgttgcgttg tataccacaa atccatggcg
    30121 cgagttttca agtattcaat tatgttaaac agcaattaga atttgaaatg aatgcggcta
    30181 atgataatcc acttatattt gaggaagcaa atgaaacgtt tgttatttca ggtggtaact
    30241 tccatggaca acctattgct tttgcattag atcatcttaa attaggtgta agtgaattag
    30301 caaacgtatc ggaacgtcgt ctagagcgac tagtaaatcc tcaattaaat ggtgatttac
    30361 cagcatttct tagtccagag ccaggattgc aaagtggcgc gatgattatg caatatgctg
    30421 ctgcaagtct cgtttctgaa aataaaactt tagcgcatcc agcgagtgtt gattctatca
    30481 cttcatctgc gaaccaagaa gatcacgtat ctatgggaac tacagctgct agacatggtt
    30541 atcaaattat tgaaaatgca agacgtgtgt tggcaatcga atgtgttatt gcattacaag
    30601 cagcagagtt gaaaggtgtc gaaggattat caccaaaaac acgtcgtaaa tatgaggagt
    30661 ttcgaagtat cgtgccatcc attacacatg accgtcaatt tcataaagat attgaagcgg
    30721 ttgcacagta tttaaagcaa tcaatttatc aaacgactgc atgtcactaa atcgacatga
    30781 gtgatgtttg aaatcgttac ttgaaaaagc aaatatagtt tgctatatta aaattaactt
    30841 aataagacat tgttcgtagg acaagtaata tatagtgttc gatatcagag agcttgtggt
    30901 tagtgtgaac aagaatcaac atatatatga atctacctac ttaatttaaa agaacaatcg
    30961 gtgataaccg ttattttagt gaagtgcaat ttaggtttag tgtatcttta taacttaaat
    31021 tgttaaatag ggtggcaacg cgtagaccac gtcccttgta gggatgtggt ctttttttat
    31081 tttctaaaaa ataaagacgt acgtcattcc ataataaatg ataattattt gggaaaggat
    31141 gaaggttaat gttagacatt agattattca gaaatgagcc tgacacagtt aagagcaaaa
    31201 ttgaattacg tggagatgat ccaaaagttg tagatgaaat tttagaattg gatgagcaac
    31261 gacgtaaatt aattagtgca acagaagaaa tgaaagcacg tcgtaataaa gtaagcgaag
    31321 aaatcgcatt aaaaaaacgt aataaagaaa atgctgatga tgtgattgct gaaatgcgca
    31381 cattaggtga cgatattaaa gaaaaagata gtcaattaaa tgaaattgat aataaaatga
    31441 caggtatcct ttgtcgtatt ccaaatttaa taagtgatga tgtacctcaa ggtgaatctg
    31501 atgaagataa cgttgaagtt aaaaagtggg gtacaccacg tgagttttca tttgaaccaa
    31561 aagcacattg ggatattgta gaagaattga aaatggctga ttttgatcgt gcagcaaaag
    31621 tttcaggtgc gcgttttgta tatttaacaa atgaaggtgc gcaattagag cgtgctttaa
    31681 tgaactatat gattacaaaa catacaacac aacatggtta tacagaaatg atggtaccac
    31741 agcttgtgaa cgcagataca atgtatggta caggtcaatt acctaaattt gaagaagatt
    31801 tatttaaagt agaaaaagaa ggattatata caattccaac tgctgaagta ccattaacga
    31861 atttctaccg taatgaaatt attcaaccag gtgtacttcc tgaaaaattc actggtcaat
    31921 ctgcatgttt ccgtagtgaa gcaggatcag caggtagaga tacaagagga ttaattcgtt
    31981 tacatcaatt cgataaagtg gaaatggtac gttttgaaca acctgaagat tcatggaatg
    32041 ctttagaaga aatgacaaca aatgcagaag caattctaga agagttaggt ttaccatacc
    32101 gtcgtgttat tttatgtaca ggtgatattg gatttagtgc aagcaaaaca tatgatatag
    32161 aagtttggtt accaagctac aatgattata aagaaattag ttcatgctca aactgtacgg
    32221 atttccaagc gcgtcgtgct aacatccgct tcaagcgtga caaagcagct aaaccagaat
    32281 tagcacatac attaaatggt agtggtttag cagttggacg tacatttgct gctattgttg
    32341 aaaattacca aaatgaagat ggaacagtaa caattccaga agcattagta ccatttatgg
    32401 gtggtaaaac acaaatttca aaaccagtta aataaaggct ttagctacaa gctttaaaaa
    32461 gtatatattc tacgtatact taaagcaagg gcaagatact ttaaataata ttttaaaaag
    32521 tggtgacgaa gctgtcgcca ctttttttgt gctgtaaaag tataatagtg agccaatgca
    32581 cataacaaca ataaattaag tttgtggttt aatggggtga acgcatttca ttatagcaac
    32641 aatacgggat aattatgatg aactaaaaca atctaaaacg taacaatttt gagcatcact
    32701 attataggaa attaagtgat aaaaaactga tttcgttgat gtagtatgag taatatcgat
    32761 ggagtagggt agggggagag gatgatgatt ataagggagt gttacatgaa tca
//
LOCUS       DANBWH010000007        32612 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.007, whole genome shotgun sequence.
ACCESSION   DANBWH010000007 DANBWH010000000
VERSION     DANBWH010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 32612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32612
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.007"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            197..643
                     /locus_tag="PPY92_000230"
     CDS             197..643
                     /locus_tag="PPY92_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDJ2624261.1"
                     /translation="MYVENSYLSKQLCFLFYVSSKEIIKKYTNYLKEYDLTYTGYIVL
                     MAIENDEKLNIKKLGERVFLDSGTLTPLLKKLEKKDYVVRTREEKDERNLQISLTEQG
                     KAIKSPLAEISVKVFNEFNISEREASDIINNLRNFVSKNFDYSDRK"
     gene            859..1287
                     /locus_tag="PPY92_000231"
     CDS             859..1287
                     /locus_tag="PPY92_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HDJ2624262.1"
                     /translation="MILNFNQFENQNFFNGNPSDTFKDLGKQVFNYFSTPSFVTNIYE
                     TDELYYLEAELAGVNKEDISIDFNNNTLTIQATRSAKYKSEQLILDERNFESLMRQFD
                     FEAVDKQHITASFENGLLTITLPKIKPSNETTSSTSIPIS"
     gene            complement(1394..2563)
                     /locus_tag="PPY92_000232"
     CDS             complement(1394..2563)
                     /locus_tag="PPY92_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NarK/NasA family nitrate transporter"
                     /protein_id="HDJ2624263.1"
                     /translation="MYKTKGGFQLTLQTLSLVVGFMAWSIIAPLMPFIKQDVNVTEGQ
                     ISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFTSFIVLLFPIFFLSQAQTPGMLMAS
                     GFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIYGMGNIGTAVSSFLAPPIAGIIGWQ
                     TTVRSYLIIIALFALIMFIFGDTQERKIKVPLMAQMKTLSKNYKLYYLSYWYFITFGA
                     FVAFGIFLPNYLVNHFGIDKVDAGIRSGVFIALATFLRPIGGILGDKFNAVKVLMIDF
                     VIMIIGAVILGISDHIALFTVGCLTISICAGIGNGLIFKLVPSYFSNEAGSANGIVSM
                     MGGLGGFFPPLVITYVANLTGSSHLAFIFLAVFGCIALFTMRHLYQKEYGSLKHS"
     gene            2932..3846
                     /locus_tag="PPY92_000233"
     CDS             2932..3846
                     /locus_tag="PPY92_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HDJ2624264.1"
                     /translation="MYKINIIRSDSVYKNILKAPINDRDSIFTKEILVPFKKKFEVQH
                     MPIYNDDKQTMSAIQFLDAFQISPKDLRMSDQMSIQYLNNDFWSNCEKYLKVAIDQFS
                     NYSISSQVSNYHFTVLLGDSQKPLMYLNKNRGGDGGIPGYIMIYLVPSTSTINSMKSL
                     IAHEVNHNMRYQYIDWDGGSLIELIIAEGLAENYVESLYGKAHIGPWVTNTNWSRDNV
                     KIKNTIYNHLHLKHIFESMPYLYGDDINKLQGRPIVGLSHAAGYACGYHLVKYFLQKT
                     NIPIEVATTLPAQKIINEVTEFWHTHTL"
     gene            3825..4565
                     /locus_tag="PPY92_000234"
     CDS             3825..4565
                     /locus_tag="PPY92_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDJ2624265.1"
                     /translation="MAYTYTLKDIIEITGVTKRTLHYYDEIGLLVPDKNDKNYRVYKQ
                     QDLEKLQKILILKSFDFDIAKIKQYISYDNEQLRKLLSEQISKLDKKISDLQLIRRSV
                     CEFIKGLSLIDTSILNKTLQSQYDKEASIKYGHTKAYQSFIRRKDSLQSQDIRHKLTT
                     IFNKFNHMSLSHYPIQDCSDLVFEWKAFMNTIADFDDETLCCIAKTYEDDTRFKDYFN
                     SYDNQNLASYISEAVNYFLSNVNKSDNF"
     gene            complement(4823..5476)
                     /gene="nreC"
                     /locus_tag="PPY92_000235"
     CDS             complement(4823..5476)
                     /gene="nreC"
                     /locus_tag="PPY92_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002464689.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation response
                     regulator NreC"
                     /protein_id="HDJ2624266.1"
                     /translation="MKIVIADDHAVVRTGFSMILNYQNDMEVVATAADGVEAYQKVME
                     YKPDVLLMDLSMPPGESGLIATSKIADSFPETKILILTMFDDEEYLFHVLRNGAKGYI
                     LKNAPDEQLLLAIRTVYKGETYVDMKLTTSLVNEFVSNSNQDTANTTDPFKILSKREL
                     EILPLIAKGYGNKEIAEKLFVSVKTVEAHKTHIMTKLGLKSKPELVEYALKKKLLEF"
     gene            complement(5499..6533)
                     /gene="nreB"
                     /locus_tag="PPY92_000236"
     CDS             complement(5499..6533)
                     /gene="nreB"
                     /locus_tag="PPY92_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501138.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation sensor histidine
                     kinase NreB"
                     /protein_id="HDJ2624267.1"
                     /translation="MINEDSIQLDTLLKKYYEHSIEKIVFADDNGKIIAMNDAAKDIL
                     SEEDNYSAVANAICHRCEGYTNAYDVQSCKDCFLESMQVQATNFQVFMKTKDQKVMPF
                     TATYQLIDQDRGIHAFTLQNVSSQIEQQEKLHQQRMMRKTISAQENERKRISRELHDS
                     VIQEMLNVDVQLRLLKYQEDTTKLLEDAENIEYIVAKLIDDIRNMSVELRPASLDDLG
                     LEAAFKSYFKQFEENYGIKIIYTSNIKNTRFDSDIETVVYRVVQEAILNALKYADVNE
                     INVGIRQTGRHLVAEVIDAGNGFDPSSKPKGSGLGLYGMNERAELVSGSVNIETKIGE
                     GTNVTLNIPI"
     gene            complement(6557..7009)
                     /gene="nreA"
                     /locus_tag="PPY92_000237"
     CDS             complement(6557..7009)
                     /gene="nreA"
                     /locus_tag="PPY92_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation accessory nitrate
                     sensor NreA"
                     /protein_id="HDJ2624268.1"
                     /translation="MTPEAIIEDRRFQETLDKIRKEEGYDFAAIAFYESNKPSSPIKW
                     HYVSGNKNNRFKLIILRKGRGLAGTVMKTGKRMVIANVGLALGPEEKIDYPILLSESL
                     TAVLAVPLWYKNQVYGVLLFGQRDGRPLPKIFDNDDIQRKFGIFNDDK"
     gene            complement(7029..7706)
                     /gene="narI"
                     /locus_tag="PPY92_000238"
     CDS             complement(7029..7706)
                     /gene="narI"
                     /locus_tag="PPY92_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDJ2624269.1"
                     /translation="MLNQFLWVIFPYLCLVVFVAGHIARYRYDKFSWTAKSSELIERK
                     RLMWGSLLFHLGIIPVFFGHVVGLLIPKSWMDAVGVSEHLYHIGAVYIGSIFGIITLI
                     GMFLLTARRVTTKSIRRLSSASDIFVNFLLLLIVFMGCYATLVTNIQHPDFNYRTSLS
                     IWFRQLFMLKPDASLMSGVPIAFKMHILLGFTIMACWPFTRLVHVWSVPLTYINRRYI
                     IYRKNKV"
     gene            complement(7699..8274)
                     /gene="narJ"
                     /locus_tag="PPY92_000239"
     CDS             complement(7699..8274)
                     /gene="narJ"
                     /locus_tag="PPY92_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDJ2624270.1"
                     /translation="MINFDNFKKYQESFGYMAQQLCFPEKLTFHPKTFEETISKDHPA
                     YEDLVAFRNVMMTFSLSEIKAIYTDTFDFTKKAPLYMTYNKFDTQKERGQMLAKLKVL
                     YEMFGLEMVDNELSDYLPLMLQFLQVAEWRNDPRAEENIQLIIMIIEDGTYVMANALG
                     EDNNPYAYVIQALRKTLKLCLTSPKGVKHHA"
     gene            complement(8267..9826)
                     /gene="narH"
                     /locus_tag="PPY92_000240"
     CDS             complement(8267..9826)
                     /gene="narH"
                     /locus_tag="PPY92_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDJ2624271.1"
                     /translation="MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNV
                     ETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPLIKDY
                     YEPWNYNYEHLTTAKSGKHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPN
                     IQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACR
                     GWRYCMTGCPYKKVYFNWKTNKAEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYD
                     ADRVHEAASAVDEKGLYEKQLDIFLNPFDEEVIAQAEKDGIGYDWIEAAQNSPIYKLA
                     IEYKLAFPLHPEFRTMPMVWYCPPLSPIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYL
                     ANIFTAGDTEPVKGALQRMAMMRSYMRSQVTQQPFDTSRLERLGITERQTKDMYRLLG
                     LAKYEDRFVIPTSHKETYLDTYHAQGSTGYNYGGEHFGDNCEGCGVAVGSGKTGQEIY
                     NENFYGGIFRD"
     gene            complement(9816..13505)
                     /locus_tag="PPY92_000241"
     CDS             complement(9816..13505)
                     /locus_tag="PPY92_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HDJ2624272.1"
                     /translation="MGKFGLNFFKPTEKFNGNWSILESKSREWEKMYRERWSHDKEVR
                     TTHGVNCTGSCSWKVFVKNGVITWENQQTDYPSCGPDMPEYEPRGCPRGASFSWYEYS
                     PLRIKYPYIRGKLWDLWTEALEENYGNRVAAWASIVENEDKAKQYKQARGMGGHVRSN
                     WKDVTEIIAAQLLYTIKKYGPDRIAGFTPIPAMSMISYAAGARFINLLGGEMLSFYDW
                     YADLPPASPQIWGEQTDVPESSDWYNASYIIMWGSNVPLTRTPDAHFMTEVRYKGTKV
                     ISVAPDYAENVKFADNWLAPNPGSDAAIAQAMTHVILQEHYVNQPNERFINYAKQYTD
                     MPFLIMLDEDENGYKAGRFLRASDLGQTTEQGEWKPVIHDAISDSLVVPNGTMGQRWE
                     EGKKWNLKLETEDGSKINPTLSMAEGGYELETIQFPYFDSDGDGIFNRPIPTRQVTLA
                     NGDKVRIATIFDLMASQYGVRRFDHKLESKGYDDAESKYTPAWQEAISGVKQSVVIQV
                     AKEFAQNAIDTEGRSMIIMGAGINHWFNSDTIYRSILNLVMLCGCQGVNGGGWAHYVG
                     QEKCRPIEGWSTVAFAKDWQGPPRLQNGTSWFYFATDQWKYEESNVDRLKSPLAKTED
                     LKHQHPADYNVLAARLGWLPSYPQFNKNSLLFAEEAKDEGIESNEAILKRAINEVKSK
                     QTQFAIEDPDLKKNHPKSLFIWRSNLISSSAKGQEYFMKHLLGTKSGLLATPNEDEKP
                     EEITWREETTGKLDLVVSLDFRMTATPLYSDIVLPAATWYEKHDLSSTDMHPYVHPFN
                     PAIDPLWESRSDWDIYKTLAKAFSEMAKDYLPGTFKDVVTTPLSHDTKQEISTPYGVV
                     KDWSKGEIEAVPGRTMPNFAIVERDYTKIYDKYVTLGPVLEKGKVGAHGVSFGVSEQY
                     EELKSMLGTWSDTNDDSVRANRPRIDTARNVADAILSISSATNGKLSQKSYEDLEEQT
                     GMPLKDISSERAAEKISFLNITSQPREVIPTAVFPGSNKQGRRYSPFTTNIERLVPFR
                     TLTGRQSYYVDHEVFQQFGESLPVYKPTLPPMVFGNRDKKIKGGTDALVLRYLTPHGK
                     WNIHSMYQDNKHMLTLFRGGPTVWISNEDAEKHDIQDNDWLEVYNRNGVVTARAVISH
                     RMPKGTMFMYHAQDKHIQTPGSEITDTRGGSHNAPTRIHLKPTQLVGGYAQISYHFNY
                     YGPIGNQRDLYVAVRKMKEVNWLED"
     gene            complement(13814..14791)
                     /gene="cobA"
                     /locus_tag="PPY92_000242"
     CDS             complement(13814..14791)
                     /gene="cobA"
                     /locus_tag="PPY92_000242"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDJ2624273.1"
                     /translation="MSVDKYGKVYLIGAGPGNPNYLTKKAERLIREADVILYDRLVNP
                     LILQYANLTTEIIDVGKKPYAKHIQQEKINECIVEAARRYNKVVRLKGGDPAIFGRVQ
                     EEVDTLNNFNIAFEIVPGVTSASAAVATMQTGLTMRAVAKSVTFSTGHFKDSEENEVD
                     VNALVNGGTLAIYMGVKRLGKIIAQIQQYTDIDYPIAIVFQASCFNEFVVKGRLSNIV
                     GKLEHYAIEAKPGICIIGEVVGYTENVSTTSNPTQQFYVVSGSRHDALMLCEHLYDEG
                     YGCLLNPNDTSNGTYHSSQYDYYDAFIKQQENVTYISTDRADANTVLCH"
     gene            complement(14782..15096)
                     /gene="nirD"
                     /locus_tag="PPY92_000243"
     CDS             complement(14782..15096)
                     /gene="nirD"
                     /locus_tag="PPY92_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HDJ2624274.1"
                     /translation="METKEKIKVTTIDELTPLIGKKVIVKGKEVGLFLTESGTIHAIH
                     NICPHKQGPLSEGTVSGEYVFCPLHDQKIDLNTGIVQEPDEGCVDVYEVEVTDGNVYI
                     CL"
     gene            complement(15100..17505)
                     /locus_tag="PPY92_000244"
     CDS             complement(15100..17505)
                     /locus_tag="PPY92_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDJ2624275.1"
                     /translation="MAKQKLVMIGNGMAGIRTIEEILERANDLYDITVIGKEPYPNYN
                     RIMLSNILQNKMTVEETIMNPYEWYEEHGIELITNDPVIEVDRANQSVTTANGIEVSY
                     DKLIFATGSKAFVIPVPGSTLPSVIGWRTIDDTEQMMNIAKTKKKAIVIGGGLLGLEC
                     ARGLLDQGMEVTVLHLAEWLMEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDD
                     VEGVKLADGREIPADLVVMAVGIRPYTEVAKESGLDVNRGIVVNDVMQTSDSNVYAVG
                     ECAEHNGKVYGLVAPLYEQGKVLADHLTNKETNGYKGSTTFTSLKVSGCDLYSAGQIV
                     ENAEIKGIEIFNSVDNNYKKIFLKDGNVVGAVLYGDIDDGSRFYNMMKKGESTEDYTL
                     VSLLTKGGEEASLSIADMADDETICGCNGVDKGTIVNAITENGFTTVEEVTAKTKAGN
                     SCGKCKPQIAQILQHTLGDDFVAAKPAGICGCTDLTRDQIVTQIRAKGLKTSKEVRHV
                     LNFKNKGGCPKCRPAINYYLNMVYPHDHEDERESRFANERYHANIQNDGTFSVIPQMR
                     GGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGLYGVKKEELPNIWEDLGMRSASAYG
                     KKTRSVKSCVGKEFCRFGTQYTTRLGIRLEKTFEYIDTPHKFKMGVSGCPRSCVESGV
                     KDFGIISVENGFQIYIGGNGGTEVEKAEFLTTVETEDEVIKLCGALMQYYRETGIYAE
                     RTAPWLRRLGFENVKEVLLDPERQNELFERIMDAKKAVEAEPWEAITSNAQARKIFEV
                     EKV"
     gene            complement(17571..18302)
                     /locus_tag="PPY92_000245"
     CDS             complement(17571..18302)
                     /locus_tag="PPY92_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HDJ2624276.1"
                     /translation="MNGNIIVAHGMRHGRQNQALEAFISELVKDDIHHYDIAFLESEH
                     QDLETVMTTLIQSGVDHFKIVPLLIFSAMHYLKDIPNIVHEMKRRYPDIKVEVSEPLG
                     THPLMRRLIEQRICDALAGDEQQVGVMVVAHGNINGNFTKAHEELQICAQHLQINKPT
                     YARTLYGEISFTHDLESISKRYQKLIVVPLFLYDGRLVNKVKQQMNDMVINTDIHFTP
                     SINFDPILKQIINDRLESLMIPMKI"
     gene            complement(18532..19068)
                     /locus_tag="PPY92_000246"
     CDS             complement(18532..19068)
                     /locus_tag="PPY92_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDJ2624277.1"
                     /translation="MTKTMIRLASEQDAEKLQQLMHEAFTPLRELGIDWPSVNANLDA
                     VKENIDKNTTFVMTIDDEIISTITVRYPWGSVKSISGYPFVWWFATNPEYEGKGYGSQ
                     LLTYVEEAFLRDTLKSAAVTLGTSARLHPWLLKIYEKRGYEIYSEHENDDGDLGVIMR
                     KVLIPERFDETILGQPPF"
     gene            complement(19302..20126)
                     /locus_tag="PPY92_000247"
     CDS             complement(19302..20126)
                     /locus_tag="PPY92_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDJ2624278.1"
                     /translation="MIENKKTVEDTYSTGAIVDSISSSVQMKQVMVQQTPGRYMLKAM
                     MAGFLLSIVTVFMFGIKTQFANTHVDGLINLMGAIAFSLGLILVVLTNSELLTSNFMY
                     FTVGWYYKVVSVKKMTWILLYCFLGNILGGFVLFFLMKFAHVMTPEMTQALTALVQKK
                     TVDSTWLNIFTKGIFCNFFINIGIFISMQFKGGLTKAFFIACGVVVFVYMGYEHVVFN
                     AGLYAGMVFFNLDAVSWLHVLKNIVFAFLGNFVGGGIFVGLVYAFLNGKRNRLEQQ"
     gene            complement(20343..20525)
                     /locus_tag="PPY92_000248"
     CDS             complement(20343..20525)
                     /locus_tag="PPY92_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624279.1"
                     /translation="MVERYIKVLILYIFTTLLSSISVTSKCVPNKVIRFILRTAIGYS
                     IFAYGLHYFSNLKKNK"
     gene            complement(20609..21076)
                     /locus_tag="PPY92_000249"
     CDS             complement(20609..21076)
                     /locus_tag="PPY92_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HDJ2624280.1"
                     /translation="MTIKVEDNKIIFSRTIEAPIEKVFDAYTTKELFEKWFHPKGAST
                     KVFRFNAVSGGDAFYAIKTPTMTSYTLAEYKTVKRPYLIEYIDSFATPQGAKDTKMPS
                     MKITLSFSKSNTTKTTVTSTSVFPTKEAAQQVINMGVEQGMNQTLDQLDALLK"
     gene            complement(21262..22809)
                     /gene="adcA"
                     /locus_tag="PPY92_000250"
     CDS             complement(21262..22809)
                     /gene="adcA"
                     /locus_tag="PPY92_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HDJ2624281.1"
                     /translation="MKKKLGMLLLVPAVTLSLAACGNDDGKDKDGKVTIKTTVYPLQS
                     FAEQIGGKHVKVSSIYPAGTDLHSYEPTQKDILSASKSDLFMYTGDNLDPVAKKVAST
                     IKDKDKKRSLEDKLDKAKLLTDQHEHGEEHEHEGHDHEKEEHHHHGGYDPHVWLDPKI
                     NQTFAKEIKDELVKKDPKHKDDYEKNYKKLNDDLKKIDNDMKQVTKDKQGNAVFISHE
                     SIGYLADRYGFVQKGIQNMNAEDPSQKELTKIVKEIRDSNAKYILYEDNVANKVTETI
                     RKETDAKPLKFYNMESLNKEQQKKDNITYQSLMKSNIENIGKALDSGVKVKDDKAESK
                     HDKAISDGYFKDEQVKDRELSDYAGEWQSVYPYLKDGTLDEVMEHKAENDPKKSAKDL
                     KAYYDKGYKTDITNIDIKGNEITFTKDGKKHTGKYEYNGKKTLKYPKGNRGVRFMFKL
                     VDGNDKDLPKFIQFSDHNIAPKKAEHFHIFMGNDNDALLKEMDNWPTYYPSKLNKDQI
                     KEEMLAH"
     gene            complement(22998..23069)
                     /locus_tag="PPY92_000251"
                     /pseudo
     CDS             complement(22998..23069)
                     /locus_tag="PPY92_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23104..23370)
                     /locus_tag="PPY92_000252"
     CDS             complement(23104..23370)
                     /locus_tag="PPY92_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDJ2624282.1"
                     /translation="MARLNITFSPQAFEDYKYFQQNNKKMVKKINELLKSIDRNGALE
                     GIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIKIASCKYHY"
     gene            complement(23370..23621)
                     /locus_tag="PPY92_000253"
     CDS             complement(23370..23621)
                     /locus_tag="PPY92_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDJ2624283.1"
                     /translation="MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESD
                     YNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKDIDV"
     gene            complement(23786..23887)
                     /locus_tag="PPY92_000254"
                     /pseudo
     CDS             complement(23786..23887)
                     /locus_tag="PPY92_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23894..24493)
                     /locus_tag="PPY92_000255"
     CDS             complement(23894..24493)
                     /locus_tag="PPY92_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDJ2624284.1"
                     /translation="MTKKLLTLFIVSMLILTACGKKESATTSSKNGKPLVVIYGDYKC
                     PYCKELDEKVMPKLRKNYIDNHKVEYQFVNLAFLGKDSIVGSRASHAVLMYAPKSFLD
                     FQKQLFAAQQDENKEWLTKELLDKHIKQLHLDKETENKIIKDYKTKDSKSWKAAEKDK
                     KIAKDNHIKTTPTAFINGEKVEDPYDYESYEKLLKDKIK"
     gene            complement(24512..24874)
                     /locus_tag="PPY92_000256"
     CDS             complement(24512..24874)
                     /locus_tag="PPY92_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HDJ2624285.1"
                     /translation="MKRFVATVLLLLVFISGCGNDKYVKEIDEAVKIQNQKQEQLAKK
                     GNGDRVDHFERKDANIYVYDKDKIIILAYKPLSNDDEVHYYAYDFSDKRVSYKQDFDS
                     RRYYQQHDADYHEENMTN"
     gene            25129..26379
                     /gene="fmhA"
                     /locus_tag="PPY92_000257"
     CDS             25129..26379
                     /gene="fmhA"
                     /locus_tag="PPY92_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhA"
                     /protein_id="HDJ2624286.1"
                     /translation="MNFVTLTSDEFNAFTTKHFSHYTQSAIHYNHRVDLKGDVHLVGV
                     KDDNGQVIAGCLLTEARTLKFFKYFYTHRGPVMDYTNQSLVAFFFKALTSYLKKHNCL
                     YVLVDPYLIENLRNADGEIVKSYDNRAFVRTMDKLGYKHQGFPVGYDSMSQIRWLSVL
                     DLKDKTEDQLLKEMDYQTRRNIKKTYDIGVKTKTLTIDETQTFFDLFHMAEEKHGFKF
                     RELPYFEEMQKLYDDHAMLKLAYIDLNEYLKTLQLKQQQLTAELSGVEEALEESPNSK
                     KNKTKRTQLEQQLNSNKRKIDNTIEQIEQDGAVLNLASALFIYNEHEVYYLSSGSNPK
                     YNAYMGAYHLQWEMIKFAKAHHIDRYNFYGITGDYSESSEDYGVQQFKKGFNAHVEEY
                     IGDFIKPIKPLLYKVQTYLNHKRR"
     gene            complement(26476..27207)
                     /locus_tag="PPY92_000258"
     CDS             complement(26476..27207)
                     /locus_tag="PPY92_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDJ2624287.1"
                     /translation="MIQLNNIHKSFNDVEVIKGIDLSVEQGEVVTLIGRSGSGKTTLL
                     RMINALEIPTEGTVYVNGKTYTSKDKKSQIEVRKQSGMVFQSYNLFPHKTALENVMEG
                     LITVKKLKKDEARGKSLELLEKVGLTHVKDQRPHALSGGQQQRVAIARALAMNPKVML
                     FDEPTSALDPELVNDVLKVIKDLANEGMTMVIVTHEMRFAKEVSNNIVFIHEGMIGEQ
                     GAPEEMFNRPKTEELRRFLNVINEE"
     gene            complement(27204..27923)
                     /locus_tag="PPY92_000259"
     CDS             complement(27204..27923)
                     /locus_tag="PPY92_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDJ2624288.1"
                     /translation="MFLNLNSEQQHALDAAKQAFGPMLEGLVKYSIPITLVTFVLGLI
                     IALFTALMRISTSKILRSISRVYVSIIRGTPMIVQLFIIFYGIPELGRLLTNDADNQW
                     TLAPVVAAIIGLSLNVGAYASEIIRGGIISIPKGQTEAAYSIGMTYGQTIQRIILPQA
                     IRVSIPALGNTFLSLIKDTSLLGFILVAEMFRKAQEVASTTYEYLTIYVLVALMYWVV
                     CFIISIIQGIYESYIERGYRS"
     gene            complement(27904..28683)
                     /locus_tag="PPY92_000260"
     CDS             complement(27904..28683)
                     /locus_tag="PPY92_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDJ2624289.1"
                     /translation="MKRLLFVMIAFVFILAACGNNSSKDKEANKDSKTINVGTEGTYA
                     PFSFHDKDGKLTGYDIDVIKAVAKEEGLKLKFNETSWDSMFAGLDAGRFDVIANQVGI
                     NPDREKKYKFSKPYTFSSAVLVIRENEKDIKDFDDVKGKKLAQTFTSNYGKLAKDKGA
                     DITKVDGFNQSMDLLLSKRVDGTFNDSLSYLDYKKQKPNAKIKAIKGNAEQSRSAFAF
                     SKKADDETVQKFNDGLKKIEENGELAKIGKKWFGQDVSKSK"
     gene            complement(28804..30243)
                     /gene="mdeA"
                     /locus_tag="PPY92_000261"
     CDS             complement(28804..30243)
                     /gene="mdeA"
                     /locus_tag="PPY92_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdeA"
                     /protein_id="HDJ2624290.1"
                     /translation="MSKKQKLTMIITMLMGGFFGLLNETLLVTALPSIMKDFEISYTQ
                     VQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVT
                     RIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYLN
                     WRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISE
                     KGFDNPIVLVSMIGGVVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSM
                     IGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRKLALVGMA
                     IVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSM
                     TNTVQQIAGSIGTAALITILSHASKNFSPTMSDYNGMNKIDMMNQIKVDTMLHGYHAG
                     FLFALLITVVSFFCSFMLQGKKKEVDSRQ"
     gene            30417..30521
                     /locus_tag="PPY92_000262"
     CDS             30417..30521
                     /locus_tag="PPY92_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDJ2624291.1"
                     /translation="MKLDLQTARRNLNSPNIKTRKRALKIIKQHKRAK"
     gene            complement(30675..31361)
                     /locus_tag="PPY92_000263"
     CDS             complement(30675..31361)
                     /locus_tag="PPY92_000263"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDJ2624292.1"
                     /translation="MPKLILCRHGQSEWNAKNLFTGWEDVNLSEQGINEATRAGEKVR
                     ENNIAIDVAFTSLLTRALDTTHYILTESKQQWIPVYKSWRLNERHYGGLQGLNKDDAR
                     KEFGEEQVHIWRRSYDVKPPAETEEQREAYLADRRYNHLDKRMMPYSESLKDTLVRVI
                     PFWTDHISQYLLDGQTVLVSAHGNSIRALIKYLEDVSDEDIINYEIKTGAPLVYELTD
                     DLEVIDKYYL"
     gene            complement(31688..32554)
                     /locus_tag="PPY92_000264"
     CDS             complement(31688..32554)
                     /locus_tag="PPY92_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDJ2624293.1"
                     /translation="MSHNENLKLAQRGAYLSLIVYIILSIVKYVTGFVFNSAAVRADA
                     LNNMTDIIVSLAVIIGLKISIKPADRNHPYGHLKSENISSLLVSFVIMFVGIQVVIQN
                     APRLFKEDDVVPNAITIIVSLISGLVMLIVFAVNQRLAKRTKSSSLNSAAKDNLSDSL
                     VSIGTAIGLIFTQIGFPIVDIILATLLGLLIVYTGFGIFKEAIFMLSDGFNETELEAY
                     RNDILEVDEVQEVKSIKGRYHGSSVFIDVTIVVDANLSLVEAHQICDNVEHHLHKKGI
                     SSVYVHPEPDHL"
BASE COUNT        10411 a         6265 c         4609 g        11327 t
ORIGIN      
        1 atgatacaat tacatttttt tcaatttaaa aaatcagtca tatcagtaat taccgatgaa
       61 aatactaaga tatcgatata ttttgcatta tttaacattt aaattcaatt atagacaatt
      121 caataattgc gatgaaatca gtacttgaca actattacac ttcataatat tgtttatttt
      181 aacaaggagt gattggatgt atgtagaaaa cagctatctt agcaagcagt tgtgtttttt
      241 attttatgtt tcttcaaaag aaataattaa aaaatacaca aactatctta aggaatatga
      301 tttaacctat actggttaca ttgttttaat ggcgattgaa aatgatgaaa aacttaacat
      361 caaaaaatta ggtgaacgtg tattcttaga ttctggaaca ctgacaccat tactaaagaa
      421 attagaaaag aaagattacg ttgttcgaac acgtgaagag aaagatgaaa gaaacctaca
      481 aatttctcta acagaacaag gtaaagcaat aaaaagccct cttgctgaaa tttctgttaa
      541 ggtctttaat gaattcaaca tttcagaacg agaagcatca gatataatta ataatttgcg
      601 gaattttgtt tctaaaaatt ttgactattc cgacagaaag taactcccta tcataaggtg
      661 atgggcagaa gtatgcatcg ctatgtttat tcattatctt tattgcttgt ataaatctcg
      721 atgctcagtt cttttgccct gattccttat caggcacaaa tctacccatt tataactttt
      781 ttatctcccc ttttacttta tcttttttaa tactactcat ataatggctt tgtaatacaa
      841 atttctataa tggaagtgat gatattgaac ttcaatcaat tcgagaatca aaactttttt
      901 aacggtaatc caagtgatac atttaaagat ttaggtaaac aagtatttaa ttacttttca
      961 acaccttcat ttgtaacgaa tatatatgaa acagacgaat tatattactt agaagctgaa
     1021 ctagcaggtg taaataaaga agatattagt atcgatttca ataataatac gctcactatt
     1081 caagctacta gaagcgcaaa atacaaatct gaacaactca ttttagatga gcgtaacttc
     1141 gaatcattaa tgcgtcaatt tgattttgaa gctgttgata agcaacatat tactgctagt
     1201 tttgaaaatg ggttattaac cattacattg cctaaaatca aaccaagcaa tgaaactact
     1261 tcatcaacat ctattccaat ttcatagtta aatttaaaat gaatacacta ctaaagcctt
     1321 aatgtcatgc tacgaatatt gtgcatgata ttaaggcttt gtatttaact aaatgaatgt
     1381 catgtattac atatcaactg tgtttcaatg aaccatattc tttttgatat aaatggcgca
     1441 tggtaaataa tgcaatgcat ccaaataccg ctaagaaaat aaatgctaaa tggcttgaac
     1501 ctgttaaatt agctacgtat gtgatgacca acggtgggaa gaatcctcct aaaccaccca
     1561 tcattgatac gataccattt gctgatcctg cttcatttga gaagtaggat ggtactaatt
     1621 taaaaattaa gccgttacca atacctgcac aaatacttat tgttaaacag cctacagtga
     1681 ataatgcgat atggtctgaa atacctaaaa taacggcacc gataatcata ataacaaaat
     1741 caatcatcag tactttaact gcattaaatt tatcacctag tataccacct attggtctca
     1801 agaatgttgc cagcgcaatg aatacacctg aacgaatacc agcatctact ttgtcaattc
     1861 caaaatgatt tactaagtag ttaggtaaga aaataccaaa tgctacaaaa gcaccaaaag
     1921 taatgaaata ccaataactt aagtaatata atttgtagtt tttagataat gttttcattt
     1981 gggccattaa tggcacttta attttacgtt cttgtgtgtc accaaaaatg aacataatta
     2041 atgcaaataa agcgataata attaagtaac ttctaactgt tgtttgccaa ccaataatac
     2101 ccgctatcgg tggtgctaaa aatgaagaaa ctgctgtacc gatattcccc ataccataaa
     2161 taccatttgc tagacctact ttttcttttg ggaaatattt aggaactgat gtaacaccaa
     2221 cagagaaaat tgcgccacct acgccaagga aaaatcctga tgccattaac atacccggcg
     2281 tttgtgcttg gcttaagaaa aatatcggga ataacaatac gataaaactt gtaaagaata
     2341 cccatttagc accaacaata tttgttaaat aaccaaatgg cacacggagc actgatccca
     2401 aaataactgg tatcgctaaa atgattgata tttgaccttc agtaacattg acatcttgtt
     2461 taataaaggg cattaatggc gcaattatac tccaagccat aaacccaacc actaaactta
     2521 atgtttgtaa tgtaagttga aagccacctt ttgttttgta catacgtttc accttcatca
     2581 aaatcattta ttttttaata gatatcaata ctctgcctat atattagagg tcaatgttat
     2641 ttaaaaatgc tttcatttat atgaaaacat tcaatataga caaagtattg actacatata
     2701 tattttatta tgattgtgaa aaagtgaaca taaggatttt cccttattta tgaagtgatt
     2761 taccctatac aatgtccctt taagatgctt ttagacactt attcgccatc attttaatca
     2821 tattatttag gaacactcac atacttttct tatttttttc aattagttac aattactgct
     2881 tgaaaatcac gttacgttat cctttatgct gacaacataa ggaggtttat catgtataaa
     2941 atcaatatta ttagatcaga ttccgtctat aagaatatac tcaaggcacc aataaacgac
     3001 cgtgattcaa ttttcactaa agaaatatta gtacctttta aaaagaaatt tgaggtacaa
     3061 catatgccta tttataatga tgacaaacag acaatgagtg cgattcaatt tttagatgcc
     3121 tttcaaattt ctcccaaaga tttacgcatg tctgaccaaa tgtcaataca atacctgaat
     3181 aatgatttct ggagtaattg cgaaaaatat ttaaaagttg ctatagatca attttcaaat
     3241 tatagtatct catctcaagt ttctaactat catttcacag tattacttgg agatagccaa
     3301 aaaccactta tgtatctaaa taaaaatcgc ggtggtgatg gtggcatacc aggttatatt
     3361 atgatttatt tagtgccgag tacaagtaca attaattcta tgaaaagctt aattgcacat
     3421 gaagtaaatc ataatatgcg ctatcaatat attgattggg atggcggaag tttgattgaa
     3481 ttgattattg cagaaggatt agctgaaaac tatgtagaat cattgtatgg taaagcgcat
     3541 attggaccgt gggtaacaaa tactaattgg agtcgtgata acgtaaaaat taaaaatact
     3601 atttataatc atttacattt aaagcatata tttgaatcga tgccttatct ctatggtgat
     3661 gatattaata aacttcaagg taggcctatc gttggcctat ctcatgctgc cgggtatgca
     3721 tgtggctatc acttggtaaa atacttttta caaaaaacaa acatacctat tgaagttgct
     3781 acaacacttc cagcacaaaa aataataaat gaggtgactg aattttggca tacacataca
     3841 ctttaaaaga tattattgaa attacaggtg taactaaaag aactttacat tattacgatg
     3901 aaataggatt attagttcca gataaaaatg ataaaaatta tcgcgtttat aaacagcaag
     3961 acttagaaaa attacaaaag attttaatac tcaagtcttt tgattttgat atcgctaaaa
     4021 taaaacaata catttcgtat gataatgaac aattgcgaaa gttattatca gagcaaataa
     4081 gcaagttaga taaaaagatt tctgacttac aattaattag acgctctgta tgtgaattta
     4141 ttaaaggact ctctctaata gataccagca ttttaaacaa gacactacag tcacaatatg
     4201 ataaagaagc atctataaaa tatggtcata cgaaagcata tcaatcattt attagacgta
     4261 aagacagctt acaatcgcag gatatcagac ataaattgac aactatcttc aataaattta
     4321 atcatatgtc tttgagtcat tatccaatcc aagattgtag tgatctcgta tttgagtgga
     4381 aggcatttat gaacactatc gctgattttg atgatgaaac attatgctgt attgctaaaa
     4441 catatgaaga tgatacgcgt ttcaaagatt actttaattc atatgataat caaaatttag
     4501 catcatacat ttcagaagct gttaattatt ttttgagcaa tgtgaataag agcgacaatt
     4561 tttaatcctc atacagatgc tacaacactg aatacaccaa ctaaaataat tgttcaaagt
     4621 agaaaactaa tcatttattc tctaaatcaa taactataat aaaagttata cctttgcaaa
     4681 gcgaattagt ataggttacc gaaaggagaa aggattaggt tccattcgat ttataaaaaa
     4741 atatatttcg aaacatacaa aagcgccaga agataatcat ttcctctcgc gcttttatac
     4801 tgaattatat taatttcaaa ctctaaaact ctaataattt ctttttcaat gcatattcaa
     4861 ctagctcagg tttactcttt aagccaagct ttgtcataat atgcgtctta tgtgcttcta
     4921 ctgttttcac agatacaaat aatttctctg caatttcttt attcccgtaa cctttggcaa
     4981 taagtggcaa tatttctagt tctcgttttg ataagatttt aaaaggatct gttgtgtttg
     5041 cagtgtcctg atttgaatta gatacaaatt cattcactaa agatgttgta agtttcatat
     5101 ctacatatgt ttcaccttta tatacagttc gaatagctaa caataattgt tcatcaggtg
     5161 catttttcaa tatgtaacct ttcgcaccat tacgcaacac atggaacaaa tactcctcat
     5221 catcaaacat tgttaatatt agtattttag tttcaggaaa actgtcagca attttactcg
     5281 tagcgataag acctgactca cctggtggca tacttaaatc cattagtaac acatcaggtt
     5341 tatattccat tactttttgg taagcttcga cgccatctgc agccgttgca acaacttcca
     5401 tatcattttg ataatttaaa atcatagaga accccgtacg gacaacagcg tgatcatcgg
     5461 caatgactat tttcaatttt attcccccaa tgtatgtttc aaattggaat gttcaatgta
     5521 acattggtac cctcaccaat tttcgtttca atattgacgc taccgctgac taactcagct
     5581 cgctcattca ttccatataa accgagtcca gaacctttag gcttagaact tggatcaaaa
     5641 ccatttcccg catctatcac ttctgctacc aaatggcgcc cagtttgacg gatacctaca
     5701 tttatttcat ttacatcagc gtatttcaac gcatttagaa tagcttcttg cactactcga
     5761 taaacaaccg tttcaatatc actatcaaag cgagtatttt taatatttga tgtatatatg
     5821 atttttattc cataattttc ttcaaactgt ttaaaatatg atttaaaagc tgcttcaagg
     5881 cctagatcat ccaaagaagc gggtcttaat tcaaccgaca tattacgtat atcatcaatt
     5941 aatttagcga caatatattc aatattttct gcgtcttcca aaagcttagt tgtatcttct
     6001 tgatatttta ataatctcaa ttgaacatct acattgagca tttcttgaat cacactatca
     6061 tgtaactctc tagaaattcg ctttctttca ttttcttggg ctgagattgt tttacgcatc
     6121 atacgttgtt gatgtaattt ctcttgctgt tcaatttgtg atgaaacatt ttgaagcgta
     6181 aatgcatgaa ttcccctgtc ttgatcaatc aactgatatg ttgctgtaaa tggcatcact
     6241 ttttgatctt tcgtcttcat aaatacttgg aaattcgtag cttgtacttg catcgattct
     6301 aagaaacagt ctttacatga ttgtacatca taggcatttg tatacccttc acatctgtga
     6361 caaatcgcat tcgccacagc actgtaatta tcttcttcag ataatatatc tttagcagca
     6421 tcattcattg caataatttt gccgttatca tcagcaaaaa ctatcttttc gattgaatgc
     6481 tcataatatt ttttcaataa agtatctaac tgtatactgt cctcattaat catgacttac
     6541 accctaattc atctcattat ttatcatcat tgaaaatacc aaacttacgt tgaatatcat
     6601 cattatcaaa tatttttggt aaaggacgac catctctttg accaaataat agtacgccat
     6661 acacttgatt cttataccaa agcggcactg ctaaaactgc tgttaatgat tcgctcaata
     6721 aaattggata gtcaatcttt tcttcaggcc ctaaagctaa accaacattg gctattacca
     6781 tacgctttcc tgttttcata acagttccag ctaatccacg accttttctt aaaataatca
     6841 atttaaatcg attattttta ttacctgaaa catagtgcca ttttattgga gatgatggtt
     6901 tgttagattc atagaaagcg attgccgcaa aatcataacc ctcttctttg cgtattttat
     6961 ctaatgtctc ttgaaatcta cgatcttcaa ttattgcttc tggtgtcaaa tcctttcacc
     7021 tcttatgctt acactttatt cttacggtaa ataatatatc tgcgatttat atatgtcaaa
     7081 ggtacactcc aaacatgtac caaacgtgta aatggccaac aagccataat agtgaaacct
     7141 aacaatatat gcattttaaa tgcaatcggc acaccactca tcaatgacgc atctggtttt
     7201 aacataaata attgtctaaa ccaaattgat aatgaagttc tgtagttaaa atctggatgt
     7261 tgtatattgg ttactaatgt tgcgtaacat cccataaata cgataagtaa taataagaaa
     7321 tttacaaata tatccgacgc tgaacttaat cttcgaatac ttttcgtagt aacacgtctc
     7381 gctgttaata aaaacatccc tatcaaagtt attataccaa agatgctacc aatataaaca
     7441 gcgcctatat gatataaatg ctcagatacc cccactgcat ccatccatga tttcggtatt
     7501 aacaatccaa ctacgtgtcc aaaaaacact ggaataatac ctaagtgaaa taataaactt
     7561 ccccacatca acctttttct ttctattagt tcactagatt tagctgtcca agaaaattta
     7621 tcataacgat aacgtgcaat atgacctgcg acaaagacaa ctaaacataa atacggaaat
     7681 ataacccata aaaactgatt aagcatgatg tttcactcct tttggtgatg tcaaacataa
     7741 tttcaatgtt tttctaagtg cttgaatcac ataggcatat ggattattat cttcaccaag
     7801 tgcattcgcc atcacatatg ttccatcctc aataatcata atgattaatt gaatattctc
     7861 ttcagctctt ggatcatttc gccattctgc cacttgcaaa aattgaagca tcaacggtag
     7921 ataatcagaa agttcattat ctaccatttc tagtccaaac atttcatata atacctttaa
     7981 tttagctaac atttgcccac gttctttttg cgtatcaaat ttgttatacg tcatatataa
     8041 tggtgctttt ttcgtaaaat caaatgtatc tgtataaatc gctttgattt ctgataatga
     8101 aaatgtcatc attacatttc taaatgctac taaatcttca tatgctggat gatctttcga
     8161 aattgtttct tcaaaagttt ttggatgaaa agttaatttt tctggaaaac ataactgttg
     8221 tgccatatat ccaaaacttt cttgatattt tttaaaatta tcgaaattaa tcacggaaaa
     8281 tccctccata gaaattctca ttataaattt cttgaccagt tttccctgaa cctactgcaa
     8341 cgccacagcc ttcacagtta tctccaaaat gctcgccgcc gtaattgtat cctgtactac
     8401 cttgtgcgtg atacgtatct aaataggttt ctttgtgtga tgttggaata acaaatcgat
     8461 cttcatattt agctagtcct aataaacgat acatgtcttt agtttggcgc tcggttatac
     8521 ctaatcgctc taatcgagac gtgtcaaatg gctgttgagt aacttgagat ctcatataac
     8581 ttctcatcat tgccatacgt tgtagggctc cttttactgg ctctgtatct cctgcagtga
     8641 aaatattagc taagtattca ataggtaaac gcatttcttc aatggctggg aaaatcgcat
     8701 ctggattttg agttgtattt ttaccttcaa aatagctcat aattgggcta agtggtgggc
     8761 aataccaaac catcggcatc gttctaaatt caggatgtaa cggaaatgca agtttatatt
     8821 caattgctaa cttataaatt ggagagtttt gtgcagcttc aatccaatca taaccaatac
     8881 catctttttc agcttgagca atgacttctt cgtcaaatgg atttaagaat atatctaatt
     8941 gtttttcgta taaacctttc tcgtctactg ctgaagccgc ttcatgaact cgatctgcat
     9001 catataataa aacacctaag taacgcatac gtcctgtaca agtttcagag cataccgtag
     9061 gcatacccgc ctcgattctc gggaaacaga aagtacattt ttcagctttg ttcgttttcc
     9121 aattgaagta aactttctta tatggacaac ctgtcataca gtaacgccat ccacgacatg
     9181 cgtcttgatc aactaataca atgccatctt catcacgttt atacatagca cctgaagggc
     9241 acgatgcaac gcaacttgga ttcaagcaat gttcacataa acgtggtaaa tacatcataa
     9301 aagtttcgtc aaattggaat ttaatatctt cttctatttt ttggatgtta ggatcttttg
     9361 ggcctgtaac atgaccacct gctaagtcat cttcccagtt aggtccccat tcaatttcaa
     9421 tgttatcccc tgtaatttct gaatacgctc tagcaactgg cgaatgcttc cctgatttcg
     9481 cagttgttaa atgttcataa ttatagttcc atggttcata ataatctttt attaatggca
     9541 tatctgggtt ataaaaaatt ttacctaaag caatttttga aattctactt ccagatttta
     9601 attcaagttt ccctttacga tttagtaccc aaccaccttt gtagtgttct tggtcttccc
     9661 aacgtttcgg ataccctaca cctggcttcg tttctacgtt attgaaccac atgtactcag
     9721 cacctggacg atttgtccaa gtgtttttac atgtcacact acacgtatgg catcctatgc
     9781 atttatctaa atttaatacc atcgcaactt gcgctttaat cttcaagcca attaacctcc
     9841 ttcatctttc taactgctac atataaatcc ctttggttcc caattggtcc ataataatta
     9901 aagtgataac taatttgtgc gtatcctccg actagttgtg ttggtttcaa atggattcta
     9961 gtcggcgcgt tgtgtgaacc accacgtgta tctgtaattt ctgacccagg cgtttgaata
    10021 tgtttatctt gtgcatgata cataaacatt gtacctttag gcatacgatg cgaaataact
    10081 gctcttgccg ttacaacacc attacggtta tacacttcta gccaatcatt atcttggata
    10141 tcgtgttttt cagcatcttc atttgatatc caaaccgttg gaccacctct aaatagtgtc
    10201 aacatatgct tattatcttg atacattgag tgtatattcc attttccatg aggcgttaaa
    10261 taacgcagta ccaaagcatc tgtaccacct ttaattttct tatctctatt cccaaatacc
    10321 attggcggca atgtcggttt atatactggt aagctctccc caaattgttg gaaaacttcg
    10381 tgatccacat aataactttg acgtcctgtt aatgttctaa aaggtactag acgttctata
    10441 ttcgttgtaa atggtgaata tcgtcgacct tgtttatttg aacctgggaa tactgctgtc
    10501 ggtattactt ctcgtggttg tgaagttata tttaaaaacg aaattttctc agcagcacgt
    10561 tcgctagaaa tatcttttaa cggcattcca gtttgttctt caagatcttc atatgatttt
    10621 tgtgataatt taccattcgt agcagatgaa atacttagta ttgcatctgc tacattacgt
    10681 gctgtatcaa tacgcggacg attcgctctc acagaatcat catttgtatc actccacgta
    10741 cctaacatac tttttaattc ttcatattgt tcactgacac cgaaacttac accatgtgct
    10801 ccaactttcc ctttttcaag tacaggacca agcgtgacat atttgtcgta aattttagtg
    10861 tagtcgcgtt ctacaattgc aaagttaggc attgtacgtc caggtaccgc ttcaatttca
    10921 cccttcgacc aatctttcac tacgccgtat ggtgttgaaa tttcttgctt tgtatcatga
    10981 ctaagtggag ttgtcacaac atctttaaac gttccaggta aatagtcttt tgccatttct
    11041 gaaaatgctt ttgccaacgt tttataaata tcccagtctg aacgcgattc ccataatgga
    11101 tcaatagctg gattaaaagg atgtacatat ggatgcatat ctgtagatga caaatcatgc
    11161 ttctcatacc aagtcgctgc tggcaaaaca atgtcagaat ataaaggtgt tgctgtcatt
    11221 ctgaaatcta aagaaacgac taaatctaat ttccctgttg tttcctcacg ccacgtaatt
    11281 tcttctggct tttcatcttc atttggtgta gctaataacc ctgattttgt gccaagtaaa
    11341 tgcttcataa agtattcttg accttttgca gaacttgaga ttagatttga gcgccatata
    11401 aacagtgatt tcggatgatt ctttttcaaa tccggatctt ctatcgcaaa ttgcgtttgt
    11461 tttgacttaa cttcatttat cgctcgtttt aaaattgcct cattcgactc aatgccttca
    11521 tctttagctt cttctgcaaa caacaaacta tttttattaa attgtggata tgatggtaac
    11581 caaccaagtc tagctgctaa aacattataa tcagctgggt gttgatgctt taaatcctct
    11641 gttttagcta atggagattt taaacgatct acatttgact cttcatattt ccattggtct
    11701 gttgcaaaat agaaccaact tgttccattt tgcaaacgtg gtggtccttg ccagtctttc
    11761 gcaaatgcga cagtactcca tccttcaatc ggacgacatt tttcttgtcc cacatagtga
    11821 gcccaaccgc caccattcac accttgacag ccacataaca taactaagtt taagattgaa
    11881 cgataaatcg tatctgagtt aaaccaatgg ttaatacccg cacccatgat aatcattgaa
    11941 cgcccttcag tatcgatagc gttttgcgca aattctttcg ctacttgaat gacaacactt
    12001 tgttttacgc ctgaaatggc ttcttgccaa gcaggtgtat attttgattc tgcatcgtcg
    12061 tatccttttg attctaattt atgatcaaaa cgacgcacgc catattgact tgccattaag
    12121 tcaaaaattg tagcaatacg gactttgtca ccatttgcta aagtgacttg tcgagttgga
    12181 attggacgat tgaatatccc atctccatca ctatcaaagt atgggaattg aattgtttct
    12241 aattcgtatc caccttctgc cattgataat gtagggttaa ttttagaacc atcttctgtt
    12301 tctagtttta agttccactt cttaccttct tcccaacgtt gacccattgt gccattaggt
    12361 actactaaac tatcgctgat tgcatcatga ataactggct tccattcgcc ttgctctgtt
    12421 gtttgaccta agtcactcgc tcttaaaaat cgacccgctt tatatccatt ttcatcttca
    12481 tccagcatga taagaaacgg catatctgta tattgtttag cgtaatttat aaagcgttca
    12541 ttaggttgat taacataatg ttcttgtaaa ataacatgtg tcattgcttg agcaattgca
    12601 gcatctgaac caggattcgg tgctagccag ttatctgcaa atttcacatt ttctgcgtaa
    12661 tctggtgcta ctgaaatgac ttttgtacct ttatagcgga cttctgtcat aaaatgtgca
    12721 tccggagtac gtgttaaagg tacattagag ccccacataa taatgtatga tgcgttatac
    12781 cagtcacttg attcaggcac atctgtttgc tctccccaaa tttgtggaga ggcaggtggt
    12841 aaatctgcat accagtcata aaaactaagc atttcaccac caagcaaatt gatgaatcga
    12901 gcacctgctg cataactaat cattgacatc gctggaatag gtgtaaatcc tgcgattcga
    12961 tctggaccat atttttttat tgtatacagt aattgtgctg cgattatctc tgtaacgtct
    13021 ttccaatttg aacgcacgtg ccctcccata cctcgggctt gcttatattg tttggctttg
    13081 tcttcatttt caacaataga cgcccatgca gcaacgcgat taccatagtt ttcttctaat
    13141 gcttcagtcc ataaatccca gagttttcca cgaatatatg gatatttgat tcgaagcgga
    13201 ctgtattcat accaagagaa tgacgcacct cgtggacatc ctctcggttc atattcaggc
    13261 atatccggac cacaacttgg atagtcagtt tgttgatttt cccaggtaat cacaccattt
    13321 ttcacaaata ctttccaaga acatgagcct gtacagttaa caccatgtgt tgttcttact
    13381 tctttatcgt ggctccaacg ttctctgtac attttttccc attctctact tttactttct
    13441 aggatcgacc aattcccatt aaatttttct gttggcttaa agaaattcaa tccaaatttt
    13501 cccatattta tatcctccta cgtataaaaa tacgatgtgt agatgtcgtg tttttaaata
    13561 ctttaaaatg cccaagacta ttgctttaat tagattgtac attttttcac aaatataaaa
    13621 tattagggaa tcacctaatt acttaaggaa tttccctatc aataacggga tttcattgaa
    13681 ataatacaca atcatgtatg gtcgtgctta ttgccaatct aaatcgttca aatttggcac
    13741 aacgacaaat aaggcttcaa cacgaatata ttctctcggt tgaaacctta cttattcatt
    13801 tattttttat aaattagtga cataacactg tattagcatc tgcacgatcg gttgaaatat
    13861 atgttacatt ttcttgctgc ttaataaatg catcatagta atcatattgc gacgaatgat
    13921 atgtgccatt cgatgtatca tttgggttta gcaaacagcc ataaccttcg tcatataaat
    13981 gttcacagag cataagggcg tcatgtctag aaccacttac tacataaaat tgctgagtcg
    14041 gattagaagt tgttgagaca ttttcagtat aacccactac ttcgcctata atacatatac
    14101 ctggtttcgc ctcaattgcg taatgctcca gtttcccaac aatattactt aaacgcccct
    14161 taacaacaaa ctcgttaaaa cacgatgctt gaaagacaat cgctatcgga taatcaatat
    14221 ctgtgtactg ttgtatctgg gcgataattt tccctaaacg tttaactccc atatatatcg
    14281 ccaacgtacc accatttact aaagcgttga catctacttc attttcttct gaatctttaa
    14341 agtgacctgt agaaaatgtc acactcttag caacagcacg catcgttaat cctgtttgca
    14401 tcgtagcaac tgcagcactc gcagatgtca caccaggtac aatttcaaac gcaatattaa
    14461 agttgtttag tgtgtcaact tcttcttgaa ctcgtccaaa tattgctggg tcgccacctt
    14521 taagcctcac aaccttgtta tatcgacgtg ctgcttccac gatacattca tttatttttt
    14581 cttgctgaat gtgttttgca tacggctttt taccaacatc gataatttca gtagtcaaat
    14641 tcgcatattg taaaattaac ggattcacta atcgatcata tagtataaca tccgcttcac
    14701 gtattaaacg ctcagccttt ttagtcaaat aattcggatt acctggtccc gcacctatca
    14761 agtaaacctt gccatattta tctacagaca tatatatacg ttcccgtctg taacttctac
    14821 ctcataaaca tctacacaac cttcatcagg ttcttgtaca atcccagtat ttaaatcaat
    14881 cttttgatca tgaagtggac agaatacata ttcaccactc actgttccct ctgacaatgg
    14941 tccttgttta tgcggacaaa tattgtgaat cgcgtgaatt gtaccacttt cagttaaaaa
    15001 caatcctacc tctttgcctt tgacaataac cttttttcca attaggggtg ttaattcatc
    15061 tatagttgtc actttaattt tttcttttgt ttccatgtat tacaccttct ccacttcaaa
    15121 aattttacgt gcttgcgcat tgctagttat tgcttcccaa ggttcagctt cgactgcttt
    15181 tttagcatcc ataatgcgtt caaatagttc attttgtctt tctgggtcaa gtaagacttc
    15241 ttttacattt tcaaatccaa gtcttcttaa ccatggcgct gttctttcag catatatacc
    15301 tgtttcgcga taatattgca tcaaagcacc acataatttg attacttcat cttctgtttc
    15361 tacagttgtt aaaaattcag ctttttcgac ttctgtacca ccattaccac cgatatagat
    15421 ttggaatcca ttttcaactg agataatacc aaaatcttta acacctgatt caacacaact
    15481 tcttgggcag cctgatacac ccattttgaa tttatgaggt gtatcgatgt attcaaatgt
    15541 tttttctaaa cgaatgccaa gtcgtgtcgt gtattgcgta ccaaatcgac aaaactcttt
    15601 accaacacag cttttaactg agcgtgtttt cttaccataa gctgatgctg aacgcatacc
    15661 taggtcttcc catatatttg gtaattcttc ttttttaact ccatacaaac caacacgttg
    15721 cgaacctgtc actttaacta gtggcacatg atatttctta gccacttctc ctagacgaat
    15781 cagttggtct gcatctgtaa cacctccacg catttgaggt ataacagaaa atgtaccatc
    15841 attttgaata ttcgcatggt aacgttcgtt agcaaatctt gattctcttt catcttcatg
    15901 atcatgtgga taaaccatgt ttaaataata gttgattgct ggtcgacatt ttggacatcc
    15961 acctttattt ttaaagttta aaacatgtcg aacttcttta gatgttttta aacctttcgc
    16021 tcttatttgc gttacaattt gatcgcgtgt caaatcagta caaccacata taccagcagg
    16081 ttttgcggca acaaagtcat ctcctaaggt gtgctgcaat atttgagcaa tttgcggttt
    16141 acatttacca catgaattcc ccgcttttgt tttagccgtt acttcttcaa ctgttgtaaa
    16201 gccattttcc gtaatcgcat ttactatagt acctttatca acaccattac aaccacaaat
    16261 tgtttcatca tcagccatat cagcaattga tagcgatgcc tcttctccac ctttagtaag
    16321 caatgataca agtgtgtaat cttcagtgga ttcacctttt ttcatcatgt tataaaagcg
    16381 tgaaccatca tcgatatcac catatagtac tgcaccaact acattaccgt cttttaaaaa
    16441 gattttttta tagttattat caacactatt aaatatttca atacctttaa tttctgcatt
    16501 ttctacaatt tgaccagcac tatacaagtc acaaccagaa acttttaatg acgtaaatgt
    16561 tgttgatccc ttgtatccgt tcgtttcttt atttgttaaa tgatcagcta atactttacc
    16621 ttgttcatat agtggtgcaa cgagtccata aactttgccg ttatgttctg cacattctcc
    16681 aactgcatat acattgctat cacttgtttg catcacatca ttgacaacaa taccacgatt
    16741 aacatctaga cctgattctt tggctacttc tgtgtatggt cgtataccta ctgccataac
    16801 aactaagtct gccggaatct cgcgtccatc agccaattta acaccttcaa catcatcttc
    16861 tcctaagatt tcagttgtgt tggcttgcat ttcaaacttc ataccttgct tttctaaatc
    16921 tgctttaagc atatttcctg ctttacggtc tagctgcatt tccatcaacc attcagctaa
    16981 atgtaacacc gttacttcca taccttgatc taataaacca cgtgcacact ctaaacctag
    17041 taagcctcca ccaattacaa ttgctttctt tttagtcttc gcaatgttca tcatttgttc
    17101 agtatcatca atcgttctcc aaccaattac actcggtaat gtcgaacctg gaactggaat
    17161 gacgaatgct ttagaacctg tcgcaaaaat caatttatcg tatgatactt caataccatt
    17221 tgcagtagta actgattgat ttgctctatc tacttcaatt acaggatcat tggtaattaa
    17281 ctcgatacca tgttcctcat accactcata tggattcata attgtttctt caactgtcat
    17341 tttattttgt aaaatatttg aaagcatgat gcggttatag tttggataag gttctttacc
    17401 tattaccgta atatcatata aatcgttggc gcgctctaat atttcttcga ttgttcgaat
    17461 gcccgccata ccgttaccaa tcattactag tttttgcttt gccataaaat atgccccttt
    17521 actccataat atttatttca aaaaaaggta ttaatttttc gttagtgctt ttatattttc
    17581 atcggaatca ttaagctttc taatctatcg ttaatgattt gctttaaaat tgggtcgaag
    17641 ttaattgaag gtgtgaagtg tatatctgta ttaataacca tgtcattcat ttgctgcttc
    17701 actttgttaa caagtcttcc gtcatataaa aataatggta cgacaatcaa tttttgatac
    17761 cgtttcgaga tgctttctaa atcatgtgta aaactaatct ctccatatag cgttctcgca
    17821 taagtaggtt tattaatctg caaatgttga gcgcatattt gtaactcttc gtgtgcctta
    17881 gtaaaatttc cattaatatt gccgtgtgca acaaccataa ctccaacttg ttgttcgtca
    17941 cctgctaatg cgtcacaaat acgttgttca attaatcgtc tcattaaagg atgtgtgcca
    18001 agtggctcgc ttacttctac ctttatgtct ggataccgtc gtttcatttc atgaacgata
    18061 ttcggtatat ccttgagata atgcattgca ctaaagatta gcaatggtac aattttaaaa
    18121 tggtcaaccc cactttgaat caacgtcgtc attaccgtct ctaaatcctg atgctcactt
    18181 tctaaaaacg caatatcata gtgatgtata tcatctttta ctaattcaga aataaatgct
    18241 tctaacgctt gattctgtcg tccgtgcctc atgccatgtg caacaatgat attcccattc
    18301 acatttacca accctttcac acgtattgta taccaaatca ttttgttttt gtgaaaagaa
    18361 tcacattata atgtaaaatc agggaattcc ctgatgcctg tagtcatgca tattccttat
    18421 acattttccc tttttgttaa atcaaaaaaa gcgaccgata tatgaatccc tactcaacat
    18481 ttatttgagc aagcatcaat atatcggtcg cttgtagtgt atattattat cttaaaatgg
    18541 tggttggcct aatattgttt cgtcaaagcg ctcgggtatc aatactttgc gcatgatcac
    18601 acctaaatcg ccatcatcat tttcatgttc gctgtatatt tcataacctc ttttttcata
    18661 aattttaagt aaccacggat gcaatcttgc agatgtacct aaagtaactg ccgctgactt
    18721 taacgtatct cgcaaaaatg cttcttcaac ataagtaagt aattggctac catagccttt
    18781 cccttcatac tcaggatttg tcgcaaacca ccagacaaaa ggatagcccg aaatactttt
    18841 cacacttccc caaggatatc taaccgtaat cgtagatata atttcatcat caattgtcat
    18901 gacaaatgta gtatttttat ctatattttc tttaacagca tctaaattag cattaactga
    18961 aggccaatca atacctagtt ctcttagagg cgtaaatgct tcatgcatga gttgttgcaa
    19021 tttttctgca tcttgttcac ttgcgagtcg aatcatcgtt tttgtcatat taatccccac
    19081 tcttttttaa atgatttaac catattttat ttttaaaata aatatccatc aaagtgtatc
    19141 aataaattta tcacatgtca gaaagtatgc ttcatctgaa tacaccaata ctctcatgaa
    19201 acttattaaa aattactctc tcaacgtaaa aaaaccattc aaattcatga atggtttgga
    19261 agaatgattc attgttacgc tatttaatca ctacatctta attattgttg ctctaaacga
    19321 ttacgcttac catttaagaa agcataaacg agacctacaa aaataccgcc accgacaaag
    19381 ttacctaaga aagcaaaaac gatatttttt aaaacatgta accatgaaac tgcatcaagg
    19441 ttaaagaata ccatacctgc atatagacct gcattgaaca caacgtgctc atatcccatg
    19501 tatacaaaga ccacgacacc acaagctatg aagaatgcct ttgttaagcc gcctttgaat
    19561 tgcatagaga tgaaaatacc aatattaata aagaagttac agaaaatacc ttttgtaaaa
    19621 atattcaacc atgttgaatc aacagtcttt ttctgaacta aagctgttaa agcttgtgtc
    19681 atttcaggtg tcatgacatg cgcaaatttc attaagaaaa ataacacaaa cccacctaaa
    19741 atatttccta agaaacaata taataaaatc caagtcatct tcttaacaga aacgacttta
    19801 taataccagc ctactgtaaa gtacatgaag ttacttgtta ataattcaga gttagttaat
    19861 acaactaaaa tcaaacctaa actaaacgca atggctccca ttaaattgat aagtccatct
    19921 acgtgagtat tcgcaaactg cgtctttatt ccgaacataa atacagtaac gattgagagt
    19981 aaaaatccgg ccatcattgc tttaagcata tatcggccag gtgtttgctg taccattact
    20041 tgcttcattt gtactgaaga tgatattgaa tcaacaattg cgcctgttga atatgtatct
    20101 tcaactgttt tcttattttc tatcacagta cttcccccat ttactttgat caaataaaat
    20161 ttactttcta attatattag taaatcaatg taattgatag attaaatttc agaaaaatct
    20221 ttttattctc tcaaaaatgt agttttcttt cataaaaaca gcgagataaa tcgcgtgatt
    20281 ggctaatatc tttatgattt aagatattac gacgctcatt ttatctcgct ttatgtaaag
    20341 tattacttat tcttttttaa atttgaaaag taatgtaaac catatgcaaa tatactgtaa
    20401 ccaattgcag tacgtaaaat aaaccttata actttattag gtacacattt tgacgttact
    20461 gaaatagaag atagtagtgt tgtgaaaata tataatatta aaactttgat ataacgttct
    20521 accatcgaat catccctcat ttttataata ttacaagatt attataaacc gaatgaaaca
    20581 aaaaagtttt tacatatttt caacaacttt atttcaataa agcatcaagt tgatctaaag
    20641 tttgattcat accttgttca acacccatgt ttatgacttg ttgagcggct tcttttgttg
    20701 gaaataccga tgttgatgtc actgtcgttt tcgttgtatt gctcttggaa aatgacaaag
    20761 taattttcat gcttggcatt tttgtatctt ttgcaccttg aggtgtcgca aatgagtcaa
    20821 tatattcaat taaatatgga cgcttaaccg ttttatattc tgctaatgta tagctggtca
    20881 ttgtaggtgt tttaatcgca taaaatgcat caccgcctga aacagcatta aagcgaaata
    20941 cttttgtgct agcccctttt ggatgaaacc atttttcaaa taattctttt gtcgtatagg
    21001 catcaaacac cttttcaatc ggtgcttcta tcgttcttga aaaaataatt ttattatctt
    21061 caactttaat cgtcatcttc ccactcccta tcttttatat ttactcatct taacatgcat
    21121 tgagtaacat atgttaccaa accatttgtt ataaactata gtaatattaa tcattccctc
    21181 tgttgaaatt gataactacg aaataaaaaa cacactctat tcagctatta acagttgagt
    21241 agagtgtgca aacctgttga attaatgtgc taacatttct tctttgattt ggtctttatt
    21301 taattttgaa ggataatatg ttggccagtt atccatttct tttaataacg cgtcattatc
    21361 attacccata aagatatgga agtgttctgc ctttttaggt gcaatgttgt gatcgctaaa
    21421 ttggatgaat ttcggtaagt ctttatcatt accatcgacc aatttaaaca taaatctcac
    21481 gccacggtta cctttaggat atttcaatgt tttcttacca ttgtattcat atttaccagt
    21541 gtgtttctta ccatctttag taaatgtaat ttcatttcct tttatatcaa tgttagtaat
    21601 atcagtttta tatcctttgt cataataagc ttttaaatct ttagcagatt tcttcggatc
    21661 attttcagct ttatgttcca tcacttcatc aagcgtaccg tcttttaagt aagggtaaac
    21721 agattgccat tcaccagcat aatcgcttaa ttcacggtct ttaacttgct catctttaaa
    21781 atacccatca gaaattgctt tatcgtgttt actttcagct ttgtcgtctt tcactttaac
    21841 accactgtct aaagctttac cgatattttc aatattcgat ttcattaatg attgatacgt
    21901 aatattatct tttttctgtt gttctttatt taaagactcc atgttgtaga attttaaagg
    21961 cttcgcatct gtttctttac gaattgtttc agtcacttta ttcgcaacat tatcttcgta
    22021 aagaatatat ttagcattgc tatctctaat ttctttaaca attttagtta attctttttg
    22081 tgatggatct tcagcattca tattttgaat acctttttga acaaaaccat aacgatcagc
    22141 taagtatcca attgattcat gtgaaataaa tactgcatta ccttgcttat cttttgtaac
    22201 ttgcttcata tcgttatcaa ttttcttaag atcgtcgttt aattttttgt agtttttctc
    22261 atagtcatct ttatgttttg gatctttttt cactaattca tctttaattt ctttagcgaa
    22321 agtttggtta attttaggat ctaaccatac gtgtggatca tatccaccat gatgatgatg
    22381 ttcttctttc tcatgatcat gcccctcatg ttcatgctct tcaccatgct cgtgttgatc
    22441 agttaaaagc tttgctttat ctaatttatc ctctaaagac cgttttttat ctttatcttt
    22501 gatagtagat gcaactttct tagcaaccgg atctaaatta tcccctgtat acataaacaa
    22561 gtctgacttg cttgcactta atatatcttt ttgtgttggt tcatagctat gtaaatctgt
    22621 ccctgctgga tagattgatg ataccttcac gtgttttcca ccaatttgct ctgcaaatga
    22681 ttgcaatgga taaactgtcg ttttaattgt taccttgcca tctttatctt ttccatcatc
    22741 attcccacat gcggctaatg ataaagttac ggctggtaca agaagtaaca tacctaattt
    22801 ctttttcata gtcaccctcc taaatagtaa tctttacgtt ttaaaattat atactcatgc
    22861 tacttaaaat acaagcactg tttttataaa taatataaag tcgaatcata ctttaggtat
    22921 ttaccacttt tcgttttatc taatacatga ctattgtaca aatatgatac agaaaacttt
    22981 atagtattta cgaacgtcat aatataagaa aaaaagacaa tctctatcat attgctaata
    23041 gaaattgcct tttcatattg tcctaaaata acttacccta tttttatagt gtgactttta
    23101 tatttaataa tggtatttac atgatgctat ttttatatga ttgtcatcta ctgtataaac
    23161 caatctatgt tcgtgattga tacgtctact ataataccca gtcagattcg attttaactt
    23221 ttcaggctta cctatacctt ccaatgcacc atttctgtca atacttttaa gtaactcatt
    23281 aatcttcttc accatttttt tattgttctg ctgaaaatac ttataatctt caaaggcttg
    23341 aggcgaaaac gtaatattta acctagccat tatacatcta tatctttcgt tatagttttc
    23401 ccacgttcta gatctgcaat tgattgagct aagtgttcag cattatttgg gttctgttgg
    23461 aggtaaagtg tttccatcat ggagttataa tctgattctg acatgattac tacgttttta
    23521 tcatcagtag atgttacagt taccatatca ctatcatcat ttacttttgt cataagtgcc
    23581 tttaaattct gtcgagcgta tgaataattt ttaataatca tatttatcgc ctccttttaa
    23641 cacacctcaa ttgtacaaat atctgtacaa taaaacaagc gacacagaaa aaaactacta
    23701 atcaagattt cgcacgggga agaatcaata cctatcctgt actgattccc tactaaacaa
    23761 tgtttacatg ataatttcta cctatttaga cttcatacat taaaaagaca atctctatca
    23821 tatcgctaat agaaattgcc ttttcatatt gtcctaataa attgatatca cttacttatc
    23881 tcaacaatta agactatttt attttatctt ttaataactt ctcataactt tcataatcat
    23941 atggatcttc aactttctcg ccattaataa atgcagttgg tgtcgttttt atatgattat
    24001 ctttcgctat ttttttatct ttctctgcag ctttccaaga cttgctatct tttgtcttgt
    24061 aatcttttat aattttattt tccgtctctt tatctaaatg cagttgttta atatgtttat
    24121 ctaatagttc ttttgttaac cattctttat tttcatcttg ctgggcagca aataattgct
    24181 tttgaaaatc taaaaatgat ttaggtgcat acatcaatac tgcatgactc gcacgcgaac
    24241 caacaattga gtctttacct aagaaagcta aattgacaaa ttggtattcc actttgtgat
    24301 tatctatata atttttacgc aactttggca tgactttttc atctaattct ttacaataag
    24361 gacatttata gtcgccatat atgacaacta atggtttgcc gtttttcgag gatgtcgttg
    24421 ctgattcttt tttaccgcaa gctgttaaaa ttaacatgct cactataaat aatgttagta
    24481 attttttagt cataataact cctgttcata tctagttcgt catattttct tcatgataat
    24541 ccgcatcatg ttgttgataa tatcgtctcg aatcaaaatc ttgcttatat gatacacgtt
    24601 tatcactaaa atcatatgca taataatgca cttcatcatc attactcaaa ggtttataag
    24661 ctaaaatgat aattttatcc ttatcataga cataaatatt agcatctttg cgttcaaaat
    24721 gatcaacacg atcaccgttg ccttttttgg caagttgttc ttgtttttga ttttgaattt
    24781 taactgcttc atctatttct ttcacatatt tatcattacc acatcctgat ataaagacta
    24841 gtaataataa taccgtcgcc acaaatcgct tcaatgctcc cactcctact atcaacttcc
    24901 aactaacttt caatataatt aatactatac accgtcaaac attaatgcgc tacaactact
    24961 accaatgcat ttaatataaa tatccaaaga aataacagtt atgtatagtc aaagaaaaat
    25021 atatttcatt ttctatgtgt atttgtaatc agtttttgat aaaataaaaa aatagcatta
    25081 ttaataaatt caaaaattac agttcaattc agagagggat gggataaaat gaactttgta
    25141 acgttgactt cggatgagtt caatgcgttt acaacaaagc atttttcaca ttacacacaa
    25201 tcagctattc attacaatca tagagttgat ttaaaaggcg atgtgcatct tgtaggggtt
    25261 aaagatgaca atggtcaagt gattgcagga tgcttattga cagaagcacg cacacttaaa
    25321 tttttcaaat atttttatac acatcgcggg ccagtgatgg attatacaaa tcaatcatta
    25381 gtagcatttt tctttaaagc attaacgtca tatttaaaga aacacaattg tttatatgtc
    25441 cttgtagatc catatttaat tgaaaattta cgcaatgcag acggtgaaat tgttaaatct
    25501 tatgataacc gagcatttgt tagaacaatg gataaattag gttataaaca ccaaggtttc
    25561 cctgtaggtt atgattcaat gagccaaatc cgttggctgt cagtgttaga tttaaaagat
    25621 aagactgaag accaactttt aaaagaaatg gattatcaaa cgagacgtaa tattaaaaaa
    25681 acatatgata ttggtgtcaa aactaaaacg ttaacgattg atgaaacgca aacttttttc
    25741 gacttattcc atatggctga ggaaaagcac ggtttcaaat tccgtgagtt accatacttt
    25801 gaagaaatgc aaaagttata cgatgaccac gccatgttaa agttggcgta tattgattta
    25861 aacgagtatt taaaaacgtt acaattaaag caacaacaat taacagctga actctcaggt
    25921 gttgaagaag cattggaaga aagtcctaat tcaaagaaaa ataaaacgaa acgcacacag
    25981 ctagaacaac agctaaatag caataagcgt aaaatcgaca atacaataga acaaattgaa
    26041 caagatggtg cagtcttaaa cttagcttct gctttattta tctacaatga gcatgaagtt
    26101 tattacttat ctagtggttc aaaccctaaa tataatgctt atatgggtgc ctaccatttg
    26161 cagtgggaaa tgattaagtt tgctaaagcg catcatattg atcgctataa tttttatggt
    26221 attaccggtg actattcaga gtcatcagaa gactatggcg tccaacaatt caaaaaaggt
    26281 tttaacgcac atgtagaaga gtatatcggt gactttatta agcctatcaa accattacta
    26341 tataaggtac aaacgtactt aaatcataaa cgtcgttaaa atatcgctcc aagattatga
    26401 caacataaca aagaaccacg catcatgtat atggcatcat caccatacta aaacatgata
    26461 cgtggtttta tttgattatt cttcatttat aacatttaag aaacgtctta attcttctgt
    26521 tttcggacga ttgaacatct cttctggagc cccttgttct ccgatcatgc cttcatgaat
    26581 aaatacaatg ttattagata cttcttttgc aaaacgcatt tcatgtgtca caatgaccat
    26641 tgtcatgcct tcattagcca aatctttaat aacctttaaa acatcattca caagttcagg
    26701 atcaagtgca gatgttggtt catcaaacaa catcacttta gggttcattg ctagtgctct
    26761 tgcaatagca acacgttgtt gttgaccacc tgataatgca tgtggacgtt gatctttgac
    26821 atgtgttaaa ccaactttct caagtaactc aagtgatttc ccacgtgcct catccttttt
    26881 caacttttta actgtgataa gaccttccat tacattttct aatgccgtct tatgcggaaa
    26941 aaggttataa ctttgaaata ccatgccaga ctgtttacga acttctattt gtgatttttt
    27001 atctttagat gtatatgttt tgccgttaac ataaactgta ccttcagttg gaatttctaa
    27061 tgcattaatc atacgtaaca atgttgtttt acctgaacca gatcgaccga ttaaggttac
    27121 aacctcacct tgttcaacag ataaatcaat acctttgatg acttcaacat cattaaatga
    27181 tttatggata ttgttcaatt gaatcatgag cgataccctc tttcaatata agattcatag
    27241 ataccttgga taattgaaat aataaagcat accacccagt acattagcgc aactaacaca
    27301 taaattgtta aatattcata tgttgtagac gcaacttctt gagcttttct aaacatttca
    27361 gccactaaaa taaatcctaa taatgatgta tctttgatta aacttaaaaa tgtattacct
    27421 agtgcaggaa tcgacactcg aattgcctgc ggtaaaatga tacgttgtat cgtttgacca
    27481 tacgtcatac cgatggaata tgcagcttct gtttgtcctt tcggtataga aataataccg
    27541 ccacgaataa tttccgaagc atacgcacct acatttaatg ataaaccaat aatagcagcc
    27601 actacaggtg ccaatgtcca ttggttgtca gcgtcatttg ttaataatct acctaattct
    27661 ggaataccat aaaatatgat aaatagttgt actatcattg gtgttcctcg aataatagat
    27721 acatagacac gtgaaatact tcttaaaatt ttactcgttg aaattcgcat taatgctgta
    27781 aataatgcaa taatcaatcc taaaacaaat gtaactaatg taataggaat tgaatatttg
    27841 accaaacctt ctagcatagg tccgaaagct tgttttgcag catctagcgc atgttgttgt
    27901 tcgctattta gatttagaaa catcttgacc aaaccatttc ttacctattt tagctaattc
    27961 accgttttcc tcgatttttt tcaagccatc attgaatttt tgaactgttt catcatctgc
    28021 ttttttagaa aatgcaaatg cagatctact ttgttcagca ttacctttga ttgctttgat
    28081 cttagcatta ggtttttgtt ttttataatc caagtatgac agactatcat taaatgtacc
    28141 atcaacacgc ttagacaata ataaatccat tgattggtta aagccatcaa cttttgtaat
    28201 atcagcacct ttatccttag ctaatttacc ataattagat gtgaatgttt gtgctaactt
    28261 cttaccttta acatcatcaa aatctttaat atctttttca ttttcacgaa taactaaaac
    28321 agcacttgag aatgtgtaag gcttagaaaa tttatatttc ttttctctat caggattaat
    28381 acctacttgg ttcgcgatta catcaaaacg ccctgcgtct aaacctgcaa acatagaatc
    28441 ccaagaagtt tcattaaatt taagttttaa accttcttct ttagccactg ctttaataac
    28501 atcaatatcg taaccagtta atttaccatc tttatcatgg aaactaaatg gtgcataagt
    28561 cccctcagta ccaacattaa ttgtcttgct atctttatta gcttccttgt ctttcgacga
    28621 attgtttcca catgcagcca atatgaaaac gaaagctatc atcacaaata aaagtctttt
    28681 catagaatat atctccttat tcttattatt ctaatcggaa taaaaatatt ttaatctgaa
    28741 atgccggata agtcaatatg ttgaactttt gctaaaaagc tttatatagc tagcaactta
    28801 tatttattga cgagaatcaa cttctttttt cttcccttgt aacataaatg aacaaaagaa
    28861 actaacaact gtaatcaata atgcaaataa aaatccagcg tgataaccat gcaacatcgt
    28921 atcaacttta atttgattca tcatatcaat tttgttcatg ccattatagt ctgacatagt
    28981 tggagaaaag tttttgcttg catgagataa aatagtaatt aatgcagctg ttccgataga
    29041 accagcaatt tgttgtactg tgtttgtcat agatgaaccg tgtgcattca tttctggctt
    29101 taattgattc atcgtatggg tcattactgg aattaatcct aacgcgatac caaccattcg
    29161 aatcgcataa actgttgcca acatgattgt tgatgtttgt tcatccatta caacaaaata
    29221 agccgtagta ataacaacaa tcgccatacc tactaaagca agctttctag gaccaaactt
    29281 ttcatataaa gcacccgaag tcatcgacat aaatgccata acaattgctc ctggtaataa
    29341 agttaatcca gaatctaaag ctgaacgatg caataaattt tgcacaaaga taggtaaaat
    29401 cgtttccgaa ccaatcatcg ataccattgt gacacccata ataatgatac caactgtaaa
    29461 ttgtttattt ttaaatacag caaaatttaa taatggtgtt gatagccgat attgacgtaa
    29521 tacaaataat gcgactaaaa caacgcctcc aatcatagat actaatacga taggattatc
    29581 aaaacctttt tctgaaattg aactcgttcc atacaataac ccgccgaaac ctagtacaga
    29641 aaaaatgaca gaaataaaat caataggtac tttaattgta gttccaacat tttttatcgt
    29701 tttaaatcca attaaaaatg taacagctgc aattggagcg acaacatgga aaagcgatct
    29761 ccagtttaaa tattcaacaa ggtaacctga aagagtaggt ccaatagctg gtgctaaacc
    29821 aattaccaaa ccaaatatcc ccatatattt accgcgttca tgcggttgga aaacatccaa
    29881 aatcgttgtc atcatcaatg gcatcataat acctgcgcct aacgcctgaa taattctagt
    29941 aactaataat gttgcaaagt gcggactcaa gccgccgagt aatgtaccta agaaaaagat
    30001 agaaataccc actaaaaaca cttgtcttgt tgtataacgt tgtataacaa gcgcggacaa
    30061 aggaataaca atcccattag tcaataaaaa agctgttgtc agccattgaa cttgtgtata
    30121 tgaaatttca aaatctttca taatacttgg taaagccgtc actaatagtg tttcatttaa
    30181 taatccaaaa aatccaccca tcagcatagt aataatcatc gttaattttt gtttcttcga
    30241 catttaaagc ttccccctca ccatctaaaa aacgacacac atttttagat atccggtaat
    30301 tatacccatt ttccaaaaaa ttagcacgac tttattacaa tttctttaaa acgtaatcat
    30361 tactatttac atatgagtag ttatcggtca taatatattt acaaggaggg atagagatga
    30421 aattagattt acagactgct cgtcgaaatt taaatagccc taacattaaa acaagaaagc
    30481 gtgctttaaa gattattaag caacacaaaa gagcgaaata attgttttaa tttgactata
    30541 ttgtatgtct aactatattt tcgagtatcc ctagctcgtt aatataaact ctatacttaa
    30601 ctatttcctt acattacttc acatatttaa gagctgcata tacactcact ccttgtgtac
    30661 atgcagctct ttttttataa gtagtattta tctataactt ctaaatcatc cgttaattca
    30721 taaacaagcg gtgcacctgt tttaatttca taattaatga tatcttcatc tgacacatct
    30781 tcaagatatt taattaatgc gcgaattgaa tttccgtgtg cagaaactaa taccgtttga
    30841 ccatctagca aatattgtga aatatgatct gtccaaaatg gtatcactcg aactaaagta
    30901 tctttcagac tttcagaata aggcatcata cgtttatcta aatgattata tcgacgatca
    30961 gctaagtaag cttcacgttg ttcttcggtt tcagcaggtg gtttcacatc ataagaacga
    31021 cgccaaatat gtacttgttc ttctccaaat tcttttctag catcatcttt atttaagcct
    31081 tgcaatccac catagtggcg ttcatttaaa cgccagcttt tatatacagg aatccattgt
    31141 tgtttagatt cagttaaaat ataatgcgtt gtatctaaag cacgtgttaa taacgatgta
    31201 aaagctacat cgatggcaat gttattttct cttacttttt cacctgctct agtcgcttca
    31261 ttaatacctt gttcagataa attaacatct tcccatccag taaataagtt tttagcattc
    31321 cactcgcttt gtccatgacg acataaaatt aattttggca taacttataa cctctttcct
    31381 ttaatcttta ggacgttatt tcagggatat tttaagatga tttaatctta gtaattttct
    31441 tttcaatata ttttaaaatt caatgattaa atcaattatc actagacatt aaatacataa
    31501 atcctattcc ccattttcat tttttaattc ataaatgaat caataaccac ctaataacaa
    31561 atcatattat acacctttgt tcgctatttt tctaaggttt aaaaaatatt tttaggtaag
    31621 cctaaaaata gatgtaataa aaacgcctcc tcagatattt atatatctat gaagacgttt
    31681 aaatacatta tagatggtct ggttctgggt gaacgtatac tgaggaaata ccttttttgt
    31741 gcaaatgatg ttcgacattg tcacaaattt gatgcgcttc tactaaggaa aggttagcat
    31801 ctacaacaat tgtgacatca ataaacacac tacttccatg gtaacgccct ttaatacttt
    31861 taacttcttg tacttcatca acttctaaaa tatcattgcg atacgcttct aattcagttt
    31921 cattgaaacc atcactcaac ataaaaattg cttctttaaa aataccaaaa ccagtataaa
    31981 cgattagtaa gcctagtaat gttgctaaaa taatatcgac aattgggaaa ccgatttgcg
    32041 taaaaattaa tcctatcgct gttccaatgc tgactaaact atctgataaa ttatctttgg
    32101 cagccgaatt taaagaacta cttttcgttc ttttcgctag tctttgattg actgcaaata
    32161 caatcaacat tacaagacca ctgattaagc tgacgataat tgttattgcg ttaggtacaa
    32221 cgtcatcttc tttgaacaaa cgaggtgcat tttgaataac tacttggata cctacaaaca
    32281 taatgacaaa tgacaccaat aatgaagaaa tattttcaga cttcaaatgg ccataaggat
    32341 gatttcgatc ggcaggttta attgaaattt tcaatccaat aataacagct aaagaaacga
    32401 taatatctgt catattgttt aatgcgtcgg ctcttacagc tgcagagtta aagacaaaac
    32461 ccgtgacata cttaacaata gataagatta tatatacaat taaactcaaa taagcaccgc
    32521 gttgcgccaa tttaagattt tcattatgag acatgcgttg aaccaccttg aattagtata
    32581 gtaacaatat tatgaatgat tcattttaat tt
//
LOCUS       DANBWH010000008        32106 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.008, whole genome shotgun sequence.
ACCESSION   DANBWH010000008 DANBWH010000000
VERSION     DANBWH010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 32106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32106
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.008"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            229..32103
                     /gene="ebh"
                     /locus_tag="PPY92_000265"
     CDS             229..32103
                     /gene="ebh"
                     /locus_tag="PPY92_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001635093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyperosmolarity resistance protein Ebh"
                     /protein_id="HDJ2624294.1"
                     /translation="MNYRDKIQKFSIRKYTVGTFSTVIATLVFLGFNTSQAHAAETNQ
                     PASVVKQKQQSNNEQTENRESQVQNSQNSQNSQSLSATHENEQPNNSQANLVNQKVAQ
                     SSTTNDEQPASQNVNTKKDSATAATTQPDKEESKHKQNESQSANKNGNDNRAAHVENH
                     EANVVTASDSSDNGNVQHDRNELQAFFDANYHDYRFIDRENADSGTFNYVKGIFDKIN
                     TLLGSNDPINNKDLQLAYKELEQAVALIRTMPQRQQTSRRSNRIQTRSVESRAAEPRS
                     VSDYQNANSSYYVENANDGSGYPVGTYINASSKGAPYNLPTTPWNTLKASDSKEIALM
                     TAKQTGDGYQWVIKFNKGHAPHQNMIFWFALPADQVPVGRTDFVTVNSDGTNVQWSHG
                     AGAGANKPLQQMWEYGVNDPDRSHDFKIRNRSGQVIYSWPTVHVYSLEDLSRASDYFS
                     EAGATPATKAFGRQNFEYINGQKPAESPGVPKVYTFIGQGDASYTISFKTQGPTVNKL
                     YYAAGGRALEYNQLFMYSQLYVESTQDHQQRLNGLRQVVNRTYRIGTTKRVEVSQGNV
                     QTKKVLESTNLNIDDFVDDPLSYVKTPSNKVLGFYPTNANTNAFRPGGVQELNEYQLS
                     QLFTDQKLQEAARTRNPIRLMIGFDYPDGYGNSETLVPVNLTVLPEIQHNIKFFKNDD
                     TQNIAEKPFSKQAGHPVFYVYAGNQGNASVNLGGSVTSIQPLRINLTSNENFTDKDWQ
                     ITGIPRTLHIENSTNRTNNARERNIELVGNLLPGDYFGTIRFGRKEQLFEIRVKPHTP
                     TITTTAEQLRGTALQKVPVNISGIPLDPSALVYLVAPTNQTTNGGSEADQIPSGYTIL
                     ATGTPDGVHNTITIRPQDYVVFIPPVGKQIRAVVYYNKVVASNMSNAVTILPDDIPPT
                     INNPVGINAKYYRGDEVNFTMGVSDRHSGIKNTTITTLPSGWTSNLTKSDNKNGSLAI
                     TGRVSMNQAFNSDITFKVSATDNVNNTTNDSQSKHVSIHVGKISEDAHPIVLGNTEKV
                     VVVNPTAVSNDEKQSIITAFMNKNQNIRGYLASTDPVTVDNNGNVTLHYRDGSSTTLD
                     ATNVMTYEPVVKSEYQTANAAKTATVTIAKGQSFNIGDIKQYFTLSNGQAIPNGTFTN
                     ITSDRTIPTAQEVSQMNAGTQLYHIVASNAYHKDTEDFYISLKIVDVKQPEGDQRVYR
                     TSTYDLTTDEISKVKQAFINANRDVITLAEGDISVTNTPNGANVSTITVNINKGRLTK
                     SFASNLANMNFLRWVNFPQDYTVTWTNAKIANRPTDGGLSWSDDHKSLIYRYDATLGT
                     QITTNDILTMLKATTTVPGLRNNITGNEKAQAEAGGRPNYRTTGYSQSNATTDGQRQF
                     TLNGQVIQILDIINPSNGYGGQPVTNSNTRANHSNSTVVNVNEPAANGAGAFTIDHVV
                     KSNSTHNASDAVYKAQLYLTPYGPKQYVEHLNQNTGNTTDAINIYFVPSDLVNPTISV
                     GNYTNHQVFSGETFTNTITANDNFGVQSVTVPNTSQITGTVDNNHQHVSATAPNVTSA
                     TSKTINLLATDTSGNTATTSFNVTVKPLRDKYRVGTSSTAANPVRIANISNNATVSQA
                     DQTTIINSLTFTSNAPNRNYATASANEITSKTVSNVSRTGNNANVTVTVTHQDGTTST
                     VTVPVKHVIPEIVAHSHYTVQGQDFPAGNGSSAADYFKLSNGSAIPDATITWVSGQAP
                     NKDNTRIGEDITVTAHILIDGETTPITKTATYKVVRTVPKHVFETARGVLYPGVSDMY
                     DAKQYVKPVNNSWSTNAQHMNFQFVGTYGPNKDVVGISTRLIRVTYDNRQTEDLTILS
                     KVKPDPPRIDANSVTYKAGLTNQEIKVNNVLNNSSVKLFKADNTPLNVTNITHGSGFS
                     SVVTVSDALPNGGIKAKSSISMNNVTYTTQDEHGQVVTVTRNESVDSNDSASVTVTPQ
                     LQATTEGAVFIKGGDGFDFGHVERFIQNPPHGATVAWHDSPDTWKNTVGNTHKTAVVT
                     LPSGQGTRNVEVPVKVYPVANAKAPSRDVKGQNLTHGTNAIDYITFDPNTNTNGITAA
                     WANRQQPNNQQAGVQHLNVDVTYPGISAAKRVPVTVNVYQFEFPQTTYTTTVGGTLAS
                     GTQASGYAHMQNASGLPTDGFTYKWNRDTTGTNDANWAAMNKPNTAQVVNAKYDVIYN
                     GHTFATSLPAKFVVKDVQPAKPTVTETAAGAITIAPGANQTVNTHAGNVTTYADKLVI
                     KRNGNVVTTFTRRNNTSPWVKEASADNVTGIVGTNNGITVAAGTFNPADTIQVVATQG
                     SGETISDEQRSDDFTVVAPQPNQATTKIWQNGHIDITPNNPSGHLINPTQAMDIAYTE
                     KVGNGAEHSKTINVVRGQNNQWTIANKPDYVTLDAQTGKVTFNANTIKPNSSITITPK
                     AGTGHSVSSNPSTLTAPAAHTVNTTEIVKDYGSNVTAAEINNAVQVANKRTATIKNGT
                     AMPTNLAGGSTTTIPVTVTYNDGSTEEVQESIFTKADKRELITAKNHLDDPVSTEGKK
                     PGTITQYNNAMHNAQQQINTAKTEAQQVINNERATPQQVSDALTKVRAAQTKIDQAKA
                     LLQNKEDNSQLVTSKNNLQSSVNQVPSTAGMTQQSIDNYNAKKREAETEITAAQRVID
                     NGDATAQQISDEKHRVDNALTALNQAKHDLTADTHALEQAVQQLNRTGTTTGKKPASI
                     TAYNNSIRALQSDLTSAKNSANAIIQKPIRTVQEVQSALTNVNRVNERLTQAINQLVP
                     LADNSALRTAKTKLDEEINKSVTTDGMTQSSIQAYENAKRAGQTETTNAQNVINNGDA
                     TDQQIAAEKTKVEEKYNSLKQAIAGLTPDLAPLQTAKTQLQNDIDQPTSTTGMTSTSV
                     AAFNDKLSAARTKIQEIDRVLASHPDVATIRQNVTAANAAKTALDQARNGLTVDKAPL
                     ENAKNQLQHSIDTQTSTTGMTQDSINAYNAKLTAARNKVQQINQVLAGSPTVDQINTN
                     TSAANQAKSDLDHARQALTPDKAPLQNAKTQLEQSINQPTDTTGMTTASLNAYNQKLQ
                     AARQKLTEINQVLNGNPTVQNINDKVAEANQAKDQLNTARQGLTLDRQPALTTLHGAS
                     NLNQAQQNNFTQQINAAQNHAALETIKSNITALNTAMTKLKDSVADNNTIKSGQNYTD
                     ATPANKQAYDNAVNAAKGVIGETTNPTMDVNTVNQKAASVKSTKDALDGQQNLQRAKT
                     EATNAITHASDLNQAQKNALTQQVNSAQNVQAVNDIKQTTQSLNTAMTGLKRGVANHN
                     QVVQSDNYVNADTNKKNDYNNAYNHANDIINGNAQHPVITPSDVNNALSNVTSKEHAL
                     NGEAKLNAAKQEANTALGHLNNLNNVQRQNLQSQINGAHQIDAVNTIKQNATNLNSAM
                     GNLRQAVADKDQVKRTEDYADADTAKQNAYNSAVSSAETIINQTANPTMSVDDVNRAT
                     SAVTTNKNALNGDEKLVQSKTDAARAIDALPHLNNAQKADVKSKINAASNITGVNTVK
                     QQGTDLNTAMGNLQGAINDEQTTLNSQNYQDATPSKKTAYTNAVQAAKDILNKSNGQN
                     KTKDQVTEAMNQVNSAKNNLDGTRLLDQAKQTAKQQLNNMTHLTTAQKTNLTNQINSG
                     TTVAGVHTVQSNANTLDQAMNTLRQSIANNDATKASEDYVDANNDKQTAYNNAVAAAE
                     TIINANSNPEMNPSTITQKAEQVNSSKTALNGDENLATAKQNAKTYLNTLTSITDAQK
                     NNLISQISSATRVSGVDTVKQNAQHLDQAMANLQNGINNESQVKSSEKYRDADTNKQQ
                     EYDNAITAAKAILNKSTGPNTAQNAVEAALQRVNTAKDALNGDAKLIAAQNAAKQHLG
                     TLTHITTAQRNDLTNQISQATNLAGVESVKQSANSLDGAMGNLQTAINDKSGTLASQN
                     FLDADEQKRNAYNQAISAAETILNKQTGPNTAKTAVEQALNNVNSAKHALNGTQNLNN
                     AKQAAITAINGASDLNQKQKDALKAQANGAQRVSNANDVQRNATELNTAMGQLQHAIA
                     DKTNTLASSKYVNADSTKQNAYTTKVTNAEHIISGTPTVVTTPSEVTAAANQVNSAKQ
                     ELNGDERLRVAKQNANTAIDALTQLNTPQKAKLKEQVGQANRLEDVQSVQTNGQSLNN
                     AMKGLRDSIANETTVKASQNYTDASPNNQSTYNSAVSNAKGIINQTNNPTMDTSAITQ
                     ATTQVNNAKNGLNGAENLRNAQNTAKQNLNTLSHLTNNQKSAISSQIDRAGHVSEVTA
                     AKNAATELNAQMGNLEQAIHDQNTVKQGVNFTDADKAKRDAYTNAVSRAETILNKTQG
                     ANTSKQDVEAAIQNVTSAKNALNGDQNVTNAKNAAKNALNNLTSINNAQKRDLTTKID
                     QATTVAGVEAVSNTGTQLNTAMANLQNGINDKANTLASENYHDADSDKKTAYTQAVTN
                     AENILNKNSGSNLDKAAVENALSQVTNAKGALNGNHNLEQAKSNANTTINGLQHLTTA
                     QKDKLKQQVQQAQNVAGVDTVKSSANTLNGAMGTLRNSIQDNTATKNGQNYLDATERN
                     KTNYNNAVDSANGVINATSNPNMDANAINQIATQVTSTKNALDGTHNLTQAKQTATNA
                     IDGATNLNKAQKDALKAQVTSAQRVANVTSIQQTANELNTAMGQLQHGIDDENATKQT
                     QKYRDAEQSKKTAYDQAVAAAKAILNKQTGSNSDKAAVDRALQQVTSTKDALNGDAKL
                     AEAKAAARQNLGTLNHITNAQRTALEGQINQATTVDGVNTVKTNANTLDGAMNSLQGA
                     INDKDATLRNQNYLDADESKRNAYTQAVTAAEGILNKQTGGNTSKADVDNALNAVTRA
                     KAALNGAENLRNAKTSATNTINGLPNLTQLQKDNLKHQVEQAQNVVGVNGVKDKGNTL
                     NTAMGALRTSIQNDNTTKTSQNYLDASDSNKNNYNTAVNNANGVINATNNPNMDANAI
                     NDMANQVNTTKAALNGAQNLAQAKTNATNTINNAQDLNQKQKDALKTQVNNAQRVSDA
                     NNVQHTATELNGAMTALKAAIADKERTKASGNYVNADQEKRQAYDSKVTNAENIINGT
                     PNATLTVNDVNSAASQVNAAKTALNGDNNLRVAKEHANNTIDGLAQLNNVQKAKLKEQ
                     VQSATTLDGVQTVKNSSQTLNTAMKGLRDSIANEATIKAGQNYTDASPNNRNEYDSAV
                     TAAKAIINQTSNPTMEPNTITQATSQVTTKEHALNGAQNLAQAKTTAKNNLNNLTSIN
                     NAQKDALTRNIDGATTVAGVNQETAKATELNNAMHSLQNGINDETQTKQTQKYLDAEP
                     SKKSAYDQAVNAAKAILTKASGQNVDKAAVEQALQNVNSTKTALNGDAKLNEAKAAAK
                     QTLGTLTHINNAQRNALDNEITQATNVEGVNTVKAKAQQLDGAMGQLETSIRDKDTTL
                     QSQNYQDADDAKRTAYSQAVNAAATILNKTAGGNTPKADVERAMQAVTQANTALNGIQ
                     NLERAKQAANTAITNASDLNTKQKEALKAQVTSAGRVSAANGVEHTATELNTAMTALK
                     RAIADKADTKASGNYVNADANKRQAYDEKVTAAEHIVSGTPTPTLTPSDVTNAATQVT
                     NAKTQLNGNHNLEVAKQNANTAIDGLTSLNGPQKAKLKEQVGQATTLPNVQTVRDNAQ
                     TLNTAMKGLRDSIANEATIKAGQNYTDASQNKQNDYNNAVTAAKAIIGQTTSPSMIAQ
                     EINQAKDQVTAKQQALNGQENLRTAQTNAKQHLNGLSDLTNAQKDAAKRQIEGATHVN
                     EVTQAQNNADALNTAMTNLKNGIQDQNTIKQGVNFTDADEAKRNAYTNAVTQAEQILN
                     KAQGPNTAKDGVETALQNVQRAKNELNGNQNVANAKTTAKNALNNLTSINNAQKAALK
                     SQIEGATTVAGVNQVSTMASELNTAMSNLQRGINDEAATKAAQKYTEADRDKQTAYND
                     AVTAAKTLLDKTAGSNDNKVAVEQALQRVNTAKTALNGDARLNEAKNTAKQQLATMSH
                     LTNAQKANLTEQIERGTTVAGVQGIQANAGTLNQAMNQLRQSIASKDATKSSEDYQDA
                     NADLQNAYNDAVTNAEGIISATNNPEMNPDTINQKASQVNSAKSALNGDEKLAAAKQT
                     AKSDIGRLTDLNNAQRTAANAEVDQAPNLAAVTAAKNKATSLNTAMGNLKHALAEKDN
                     TKRSVNYTDADQPKQQAYDTAVTQAEAITNANGSNANETQVQAALNQLNQAKNDLNGD
                     NKVAQAKETAKRALASYSNLNNAQSTAATSQIDNATTVADVTAAQNTANELNTAMGQL
                     QNGINDQNTVKQQVNFTDADQGKKDAYTNAVTNAQGILDKANGQNMTKAQVEAALNQV
                     TTAKNALNGDANVRQAKSDAKANLGTLTHLNNAQKQDLTSQIEGATTVNGVNSVKTKA
                     QDLDGAMQRLESAIANKDQTKASENNIDADPTKKTAFDNAITQAESYLNKDHGTNKDK
                     QAVEQAIQSVTSTENALNGDANLQCAKTEATQAIDNLTQLNTPQKTALKQQVNAAQRV
                     SGVTDLKNSATSLNNAMDQLKQAIGDHDTIVAGGNYTNASPDKQGAYTDAYNAAKNIV
                     NGSPNVITNAADVTAATQRVNNAETSLNGDTNLATAKQQAKDALRQMTHLSDAQKQSI
                     TGQIDSATQVTGVQSVKDNATNLDNAMNQLRNSIANKDEVKASQPYVDADTDKQNAYN
                     TAVTSAENIINATSQPTLDPSAVTQAANQVNTNKTALNGAQNLANKKQETTANINRLS
                     HLNNAQKQDLNTQVTNAPNISTVNQVKTKAEQLDQAMERLINGIQDKDQVKQSVNFTD
                     ADPEKQTAYNNAVTAAENIINQANGTNANQSQVEAALSTVTTTKQALNGDRKVTDAKN
                     NANQTLSTLDNLNNAQKGAVTGNINQAHTVAEVTQAIQTAQELNTAMGNLKNSLNDKD
                     TTLGSQNFADADPEKKNAYNEAVRNAENILNKSTGTNVPKDQVEAAMNQVNTTKAALN
                     GTQNLEKAKQHANTAIDGLSHLTNAQKEALKQLVQQSTTVAEAQGNEQKANNVDAAMD
                     KLRQSIADNATTKQNQNYTDASPNKKDAYNNAVTTAQGIIDQTTNPSLDPTVINQAAG
                     QVSTSKNALNGNENLEAAKQQATQSLGSLDNLNNAQKQAVTNQINGAHTVDEANQIKQ
                     NAQNLNTAMGNLKQAIADKDATKATVNFTDADQAKQQAYNTAVTNAENIISKANGGNA
                     TQTEVEQAIQQVNAAKQALNGNANVQHAKDEATALINNSNDLNQAQKDALKQQVQNAT
                     TVAGVNNVKQTAQELNNAMTQLKQGIADKEQTKADGNFVNADSDKQNAYNQAVAKAEA
                     LISGTPDVVVTPSEITAALNKVTQAKNDLNGNTNLATAKQNVQHAIDQLPNLNQAQRD
                     EYSKQITQATLVPNVNAIQQAATTLNDAMTQLKQGIANKAQIKGSENYHDADTDKQTA
                     YDNAVTKAEELLKQTTNPTMDPNTIQQALTKVNDTNQALNGNQKLADAKQDAKTTLGT
                     LDHLNDAQKQALTTQVEQAPDIATVNNVKQNAQNLNNAMTNLNNALQDKTETLNSINF
                     TDADQAKKDDYTNAVSHAEGILSKANGSNASQTEVEQAMQRVNEAKQALNGNDNVQRA
                     KDAAKQVITNANDLNQAQKDALKQQVDAAQTVANVNTIKQTAQDLNQAMTQLKQGIAD
                     KDQTKANGNFVNADTDKQNAYNNAVAHAEQIISGTPNANVDPQQVAQALQQVNQAKGD
                     LNGNHNLQVAKDNANTAIDQLPNLNQPQKTALKDQVSHAELVTGVNAIKQNADALNNA
                     MGTLKQQIQANSQVPQSVDFTQADQDKQQAYNNAANQAQQIANGTPTPVLAPDTVTKA
                     VTTMNQAKDALNGDEKLAQAKQDALANLDTLRDLNQPQRDALRNQINQAQALATVEQT
                     KQNAQNVNTAMGNLKQGIANKDTVKASENYHDADVDKQTAYTNAVSQAEGIINQTTNP
                     TLNPDDITRALTQVTDAKNSLNGEAKLATEKQNAKDAVSGMTHLNDAQKQALKGQIDQ
                     SPEIATVNQVKQTATSLDQAMDQLSQAINDKDQILADGNYLNADPDKQNAYKQAVAKA
                     EALLNKQSGTNEVQAQVESITNEVNAAKQALNGNDNLANAKQQAKQQLANLTHLNDAQ
                     KQSFESQITQAPLVTDVTTINQKAQTLDHAMELLRNSVADNQTTLASEDYHDATAQRQ
                     NDYNKAVTAANNIINQTTSPTMNPDDVNGATTQVNNTKVALDGDENLAAAKQQANNRL
                     DQLDHLNNAQKQQLQSQITQSSDIAAVNGHKQTAESLNTAMGNLINAIADHQAVEQRG
                     NFINADTDKQTAYNTAVNEAAAMINKQTGQNANQTEVEQAITKVQTTLQALNGDHNLQ
                     VAKTNATQAIDVLTSLNDPQKTALKDQVTAATLVTAVHQIEQNANTLNQAMHGLRQSI
                     QDNAATKANSKYINEDQPEQQNYDQAVQAANNIINEQTATLDNNAINQVAATVNTTKA
                     ALHGDVKLQNDKDHAKQTVSQLAHLNNAQKHMEDTLIDSETTRTAVKQDLTEVQALDQ
                     LMDALQQSIADKDATRASSAYVNAEPNKKQAYDEAVQNAESIIAGLNNPTINKGNVSS
                     ATQAVISSKNALDGVERLAQDKQTAGNSLNHLDQLTPAQQQALENQINNATTRDKVAE
                     IIAQAQALNEAMKALKESIKDQPQTEASSKFINEDQAQKDAYTQAVQHAKDLINKTTD
                     PTLAKSIIDQATQAVTDAKNNLHGDQKLAQDKQRATETLNNLSNLNTPQRQALENQIN
                     NAATRGEVAQKLTEAQALNQAMEALRNSIQDQQQTESGSKFINEDKPQKDAYQAAVQN
                     AKDLINQTGNPTLDKAQVEQLTHAFKQAKDNLHGDQKLADDKQHAVTDLNQLNGLNNP
                     QRQALESQINNAATRGEVAQKLAEAKALDQAMQALRNSIQDQQQTEAGSKFINEDKPQ
                     KDAYQAAVQNAKDLINQTGNPTLDKSQVEQLTQAVTTAKDNLHGDQKLARDQQQAVTT
                     VNALPTLNHAQQQTLTDAINAAPTRTEVAQHVQTATELDHAMETLKNKVDQVNTDKAQ
                     PNYTEASTDKKEAVDQALQAAQSITDPTNGSNANKDAVEQALTKLQEKVNELNGNERV
                     AEAKTQAKQTIDQLTHLNADQIATAKQNIDQATKLQPIAELVDQATQLNQSMDQLQQA
                     VNEHANVEQTIDYTQADSDKQKAYKQAIADAENVLKQNANKQQVDQALQNILNAKQAL
                     NGDERVALAKTNGKHDIDQLNALNNAQQDGFKGRIDQSNDLNQIQQIVDEAKALNRAM
                     DQLSQEITGNEGRTKGSTNYVNADTQVKQVYDEAVDKAKQALDKSSGQNLTAEQVIKL
                     NDAVTAAKKALNGEERLNNRKAEALQRLDQLTHLNNAQRQLAIQQINNAETLNKASRA
                     INRATKLDNAMGAVQQYIDEQHLGVISSTNYINADDNLKANYDNAIANAAHELDKVQG
                     NAIAKAEAEQLKQNIIDAQNALNGDQNLANAKDKANAFVNSLNGLNQQQQDLAHKAIN
                     NADTVSDVTDIVNNQIDLNDAMETLKHLVDNEIPNAEQTVNYQNADDNAKTNFDDAKR
                     LANTLLNSDNTNVNDINGAIQAVNDAIHNLNGDQRLQDAKDKAIQSINQALANKLKEI
                     EASNATDQDKLIAKNKAEELANSIINNINKATSNQAVSQVQTAGNHAIEQVHANEIPK
                     AKIDANKDVDKQVQALIDEIDRNPNLTDKEKQALKDRINQILQQGHNDINNALTKEEI
                     EQAKAQLAQALQDIKDLVKAKEDAKQDVDKQVQALIDEIDQNPNLTDKEKQALKDRIN
                     QILQQGHNGINNAMTKEEIEQAKAQLAQALKEIKDLVKAKENAKQDVDKQVQALIDEI
                     DQNPNLTDKEKQALKDRINQILQQGHNDINNAMTKEEIEQAKAQLAQALQDIKDLVKA
                     KEDAKNAIKALANAKRDQINSNPDLTPEQKAKALKEIDEAEKRALQNVENAQTIDQLN
                     RGLNLGLDDIRNTHVWEVDEQPAVNEIFEATPEQILVNGELIVHRDDIITEQDILAHI
                     NLIDQLSAEVIDTPSTATISDSLTAKVEVTLLDGSKVIVNVPVKVVEKELSVVKQQAI
                     ESIENAAQQKIDEINNSVTLTLEQKEAAIAEVNKLKQQAIDHVNNAPDVHSVEEIQQQ
                     EQAYIEQFNPEQFTIEQAKSNAIKSIEDAIQHMIDEIKARTDLTDKEKQEAIAKLNQL
                     KEQAIQAIQRAQSISEITEQLEQFKAQMKAANPTAKELAKRKQEAISRIKDFSNEKIN
                     SIRNSEIGTADEKQAAMNQINEIVLETIRDINNAHTLQQVEAALNNGIARISAVQIVI
                     SDRAKQSSSTGNESNSHLTIGYGTANHPFNSSTIGHKKKLDEDDDIDPLHMRHFSNNF
                     GNVIKNAIGVVGISGLLASFWFFIAKRRRKEDEEEELEIRDNNKDSIKETLDDTKHLP
                     LLFAKRRRKEDEEDVTVEEKDSLNNGESLDKVKHTPFFLPKRRRKEDEEDVEVTNENT
                     DEKVLKDNEHSPLLFAKRRKDKEEDVETTTSIESKDEDVPLLLAKKKNQKDNQSKDKK
                     SASKNTSKKVAAKKKKKKSKKNKK"
BASE COUNT        13117 a         5747 c         5766 g         7476 t
ORIGIN      
        1 acaatatatt ttgtgttatt atattctgga gcataaataa attgttcaac acatagttgt
       61 aatgtgtttc aatacttttt ggatagattg cgaaattgta ttgaatcgtc atcgttttaa
      121 atttttaaat gagaatggaa tgagcattac aatatacaag caatcaaaag taaatacatt
      181 cacaacacaa cagagacata acaacaagat aaggagtgaa caatagctgt gaattatcgt
      241 gataaaattc aaaagtttag tattcgtaaa tatacagttg gtacattttc aactgtcatt
      301 gcgacattgg tatttttagg attcaataca tcacaagcac atgctgctga aacaaatcaa
      361 ccagcaagcg tggttaaaca gaaacaacaa agtaataatg aacagactga gaatcgagaa
      421 tctcaagtac aaaattctca aaattcacaa aatagtcaat cattatccgc tactcatgaa
      481 aatgagcaac caaataatag tcaagctaat ttagtaaatc aaaaagtagc gcaatcatct
      541 actactaatg atgaacaacc agcatctcaa aatgtaaata caaagaaaga ttcggcaacg
      601 gctgcgacaa cacaaccaga taaagaagaa agtaagcata aacaaaacga aagtcaatct
      661 gctaataaaa atggaaacga caatagagcg gctcatgtag aaaatcatga agcaaatgta
      721 gtaacagctt cagattcatc tgataatggt aacgtacaac atgaccgaaa tgaattacaa
      781 gcattttttg atgcaaatta tcatgattat cgctttattg accgtgaaaa tgcagattct
      841 ggcacattta actatgtaaa aggcattttt gacaagatta atactttatt aggcagtaat
      901 gatccaatta acaataaaga cttgcaactt gcatacaaag aattggaaca agctgttgct
      961 ttaattcgta caatgcctca acgtcaacaa actagccgtc gatcaaacag aattcaaacg
     1021 cgttctgttg agtctagagc tgcagagcct agatcagtat cagactatca aaatgcaaat
     1081 tcatcatatt atgttgaaaa tgctaatgat ggttcaggat atcctgtagg tacatatatc
     1141 aatgcttcta gtaaaggggc gccatataat ttaccaacta caccatggaa tacattgaag
     1201 gcctctgact caaaggaaat tgctcttatg acagcgaaac aaactggaga tggctaccaa
     1261 tgggttatta agtttaataa aggacatgct ccacatcaaa atatgatttt ctggtttgca
     1321 ttaccagcag accaagtgcc agtaggaaga actgactttg taacagttaa ttcagatgga
     1381 acaaatgtac aatggagtca tggagcagga gcaggtgcaa ataaaccact tcaacaaatg
     1441 tgggaatatg gagtaaatga tcctgatcgt tcacatgact ttaaaataag aaatagaagt
     1501 ggccaagtaa tatatagctg gccaactgtc catgtttatt ctttagaaga tttatctaga
     1561 gcgagtgatt attttagtga agctggagcg acacctgcta ctaaagcatt tggtagacaa
     1621 aattttgaat atattaatgg tcaaaaacct gctgaatcac cgggtgttcc taaagtttat
     1681 actttcatcg gtcaaggtga tgcaagttat acaatttcat ttaaaacaca aggtccaact
     1741 gttaataaat tgtattatgc agcaggtggg cgtgctttag agtacaatca attatttatg
     1801 tacagtcaac tatacgtcga atcaacgcaa gaccatcaac aacgtcttaa tggtttaaga
     1861 caagtggtta atcgtacata tcgcataggt acaactaaac gtgtagaagt gagtcaagga
     1921 aatgtacaaa cgaaaaaggt attagaaagt acaaacctaa atatagatga ttttgttgat
     1981 gatcctttaa gttatgttaa gacgccgagt aataaagtgt taggttttta cccaactaat
     2041 gcaaatacta acgcttttag accggggggc gttcaagaat taaatgaata tcaattaagt
     2101 caattattta ctgatcaaaa attacaagaa gcagcaagaa ctagaaaccc aataagatta
     2161 atgattggtt tcgactatcc tgatggttat ggtaatagtg aaactttagt tcctgttaac
     2221 ttaacggtat tacctgaaat ccaacataat attaaattct ttaaaaatga cgatactcaa
     2281 aatattgctg aaaaaccatt ttcaaaacaa gctgggcatc cagttttcta tgtatatgca
     2341 ggtaaccaag ggaatgcttc cgtgaattta ggtggtagcg taacatctat tcaaccatta
     2401 cgtattaatt taacaagtaa tgagaatttt acagataaag attggcaaat tacaggtatt
     2461 ccgcgtacat tacacattga aaactcgaca aatagaacta ataatgctag agaacgtaac
     2521 attgaacttg ttggtaattt attaccaggg gattactttg gtacgatacg ttttggacgt
     2581 aaagaacaat tatttgaaat tcgtgttaaa ccacatacac caacaattac aacgacagct
     2641 gagcaattaa gaggtacagc attacaaaaa gtgcctgtta atatttcggg aataccgttg
     2701 gatccatcgg cattggttta tttagttgca ccaacaaatc aaactacgaa tggtggtagt
     2761 gaggcagatc aaataccatc tggttatacg atacttgcga ctggtacacc tgatggggtg
     2821 cataatacaa ttactatacg accgcaagat tatgttgtat tcataccacc tgtaggtaaa
     2881 caaattagag cagtagttta ttataataaa gtagttgcat ctaatatgag taatgctgtt
     2941 actattttgc cagatgacat tccaccaaca atcaataatc ctgttggaat aaatgccaaa
     3001 tactatcgag gcgacgaagt caactttaca atgggagtct ctgatagaca ttctggtata
     3061 aaaaatacaa ctattactac tttgccaagt ggttggacat caaatttaac taaatccgac
     3121 aacaaaaacg gctcattagc tattacaggt agagtctcta tgaatcaggc atttaacagt
     3181 gatattacat ttaaagtatc agcgacagac aatgtcaata atacgacaaa tgatagtcaa
     3241 tctaaacatg tgtcaattca tgtaggtaaa attagtgaag atgctcatcc gattgtatta
     3301 ggaaatactg agaaagttgt agtagtcaat ccgactgctg tatctaatga tgaaaagcaa
     3361 agcataatta ctgcctttat gaataaaaac caaaatataa gaggatattt agcatcaact
     3421 gatccagtaa ctgtcgataa taatggtaac gtcacattac attaccgtga tggctcatca
     3481 acaacgcttg atgctacaaa tgtgatgaca tacgaaccag ttgtgaaatc tgaatatcaa
     3541 actgccaatg ctgctaaaac agcaacggta acgattgcta aaggacaatc atttaatatt
     3601 ggtgatatta aacaatattt tactttaagt aatggacaag ctattccaaa tggcacattt
     3661 acaaatatta catctgatag aactattcca actgcacaag aagttagtca aatgaatgca
     3721 ggtacgcagt tatatcatat agttgcttca aatgcatatc ataaagacac tgaagatttc
     3781 tatattagtt taaaaatcgt tgatgtgaaa caacctgaag gcgatcaacg tgtctatcgt
     3841 acgtcaacat atgatttaac cactgatgaa atctcaaaag taaaacaagc ttttattaat
     3901 gcaaatagag atgtaattac gcttgccgaa ggtgatattt cagttacaaa tacacctaat
     3961 ggtgctaatg taagtactat tacagtaaat attaataaag gtcgattaac gaaatcattc
     4021 gcgtctaacc tagctaatat gaatttcttg cgttgggtta atttcccaca agattataca
     4081 gtgacatgga cgaatgcaaa aattgcaaac agaccaacag atggtggttt atcatggtcc
     4141 gatgaccata aatctttaat ttatcgttat gatgctacat taggcacaca aattacaact
     4201 aatgatattt taacgatgct aaaagcgact actacagtgc ctggattgcg taataatatt
     4261 actggtaatg aaaaagcaca agcagaagca ggtggaagac caaactatag aacaactggt
     4321 tattcacaat caaatgcgac aactgatggt caacgtcaat ttacgttgaa tggtcaagtg
     4381 attcaaatat tagacatcat caacccttca aacggttatg gtgggcaacc tgttacaaat
     4441 tcaaatactc gtgcaaacca tagtaactca actgttgtta acgtaaacga accggcagct
     4501 aatggtgctg gcgcatttac aattgaccac gttgtaaaaa gtaattctac acataatgca
     4561 agtgatgcag tttataaagc gcagttatac ttaacgccat atggtccaaa acaatatgtt
     4621 gaacatttaa atcaaaatac aggaaatact actgacgcta ttaacattta ttttgtacca
     4681 agtgacttag tgaatccaac aatttcagta ggtaattaca ctaatcatca agtgttctca
     4741 ggtgaaacat ttacaaatac gattacagcg aatgataact ttggtgtgca atcggtaact
     4801 gtaccaaata catcacaaat tacaggtact gttgataata accatcaaca tgtttctgca
     4861 acggcaccaa atgtgacatc agcaactagt aagacaatca atttattagc aactgataca
     4921 agtggtaata cagctacaac ttcattcaat gtaacagtga aacctttgcg tgataaatat
     4981 cgagttggta cttcatcaac ggctgctaat cctgttagaa ttgccaatat ttcgaataat
     5041 gcgacagtat cacaagctga tcaaacgaca attattaatt cgttaacgtt tacaagtaat
     5101 gcaccaaata gaaactatgc aacagcaagc gcaaatgaaa tcactagtaa aacagttagt
     5161 aatgtcagtc gtactggaaa taatgccaat gtcacagtaa ctgttactca tcaagatgga
     5221 acaacatcaa cagtgactgt acctgtaaag catgtcattc cagaaatcgt tgcacattcg
     5281 cattacactg tacaaggcca agacttccca gcaggtaatg gttctagtgc agcagattac
     5341 tttaagttat ctaatggtag tgccattcca gatgcaacga ttacatgggt aagtggacaa
     5401 gcgccaaata aagataatac acgtattggt gaagatataa cagtaactgc acatatctta
     5461 attgatggcg aaacaacgcc gattacgaaa acagcaacat ataaagtagt aagaactgta
     5521 ccgaaacatg tctttgaaac agccagaggt gttttatacc caggtgtttc agatatgtat
     5581 gatgcgaaac aatatgttaa gccagtaaat aattcttggt cgacaaatgc gcaacatatg
     5641 aattttcaat ttgttggaac atatggtcct aacaaagatg ttgtaggtat atcaacgcgt
     5701 cttattagag tgacttatga taatagacaa actgaagatt taactatttt atctaaagtt
     5761 aaacctgacc caccaagaat tgacgcaaac tctgtgacat ataaagcagg tcttacaaac
     5821 caagaaatta aagttaataa cgtattaaat aactcgtcag taaaattatt taaagcagat
     5881 aatacaccat taaatgtcac aaatattact catggtagtg gttttagttc ggttgtgaca
     5941 gtaagtgacg cgttaccaaa tggcggaatt aaagcaaaat cttcaatttc aatgaacaat
     6001 gtgacgtata cgacgcaaga cgaacatggt caagttgtta cagtaacaag aaatgaatct
     6061 gttgattcaa atgatagtgc ttctgttaca gtaacaccac aattacaagc aactactgaa
     6121 ggcgctgtat ttattaaagg tggcgacggt tttgatttcg gtcatgtaga acgatttatt
     6181 caaaatccgc cacatggggc aacggtcgca tggcatgata gtccagatac atggaagaat
     6241 acagtcggca acacacataa aactgcggtt gtaacattac ctagtggtca aggtacgcgt
     6301 aatgttgaag ttccagtcaa agtttatcca gttgctaatg ctaaggcgcc atcacgtgat
     6361 gtgaaaggtc aaaatttgac acatggtaca aacgctattg attacattac atttgatcca
     6421 aatactaata cgaatggtat tacagcagca tgggcaaata gacaacaacc aaataaccag
     6481 caagcaggcg ttcaacattt aaatgtcgat gtcacatatc caggtatttc agctgctaaa
     6541 cgagttcctg taactgtgaa cgtatatcaa tttgaattcc ctcaaactac ttatacaaca
     6601 acagttggtg gcactttagc aagtggtacg caagcatcag gatatgcaca tatgcaaaac
     6661 gcttcaggtt taccaacaga tggatttacg tataaatgga atcgtgatac tacgggtaca
     6721 aacgatgcaa actgggcagc aatgaataaa ccaaatactg cacaagtcgt taatgcaaaa
     6781 tatgatgtca tctataatgg acatacattt gcaacatctt taccagcgaa atttgtagta
     6841 aaagatgttc aaccagcgaa accaactgtc actgaaacag cggcaggagc gattacaatt
     6901 gcacctggtg cgaaccaaac agtcaatact catgctggta atgttacgac atatgctgac
     6961 aaattagtta ttaaacgtaa tggaaatgtt gtaacgacat ttacacgtcg taataatacg
     7021 agcccatggg tgaaagaagc atcagcagat aatgtaacag gtattgttgg aactaataat
     7081 ggtattactg tggcagcagg tactttcaat cctgctgata caattcaagt tgttgcaaca
     7141 caaggtagtg gcgaaacaat cagtgacgag caacgtagtg atgatttcac agttgtcgca
     7201 ccacaaccga accaagcgac tacgaaaatt tggcaaaatg gtcatattga tatcacgcct
     7261 aataatccat caggacattt aattaatcca acacaagcaa tggatattgc ttacactgaa
     7321 aaagtgggta atggtgcaga acatagtaag acaattaatg ttgttcgtgg tcaaaataat
     7381 caatggacaa ttgcgaataa gcctgactat gtaacgttag atgcacaaac tggtaaagtg
     7441 acgttcaatg ccaatactat aaaaccaaat tcatcaatca caattactcc gaaagcaggt
     7501 acaggtcact cagtaagtag taatccaagt acattaactg caccggcagc tcatactgtc
     7561 aacacaactg aaattgtgaa agattatggt tcaaatgtaa cagcagctga aattaacaat
     7621 gcagttcaag ttgctaataa acgtactgca acgattaaaa atggcacagc aatgcctact
     7681 aatttagctg gtggtagcac aacgacgatt cctgtgacag taacttacaa tgatggtagt
     7741 actgaagaag tacaagagtc cattttcaca aaagcggata aacgtgagtt aatcacagct
     7801 aaaaatcatt tagatgatcc agtaagcact gaaggtaaaa agccaggtac aattacgcag
     7861 tacaataatg caatgcataa tgcgcaacaa caaatcaata ccgcgaaaac agaagcacaa
     7921 caagtgatta ataatgagcg tgcaacacca caacaagttt ctgacgcact aactaaagtt
     7981 cgtgcagcac aaactaagat tgatcaagct aaagcattac ttcaaaataa agaagataat
     8041 agccaattag taacgtctaa aaataactta caaagttctg tgaaccaagt accatcaact
     8101 gctggtatga cgcaacaaag tattgataac tataatgcga agaagcgtga agcagaaact
     8161 gaaataactg cagctcaacg tgttattgac aatggcgatg caactgcaca acaaatttca
     8221 gatgaaaaac atcgtgtcga taacgcatta acagcattaa accaagcgaa acatgattta
     8281 actgcagata cacatgcctt agagcaagca gtgcaacaat tgaatcgcac aggtacaacg
     8341 actggtaaga agccggcaag tattactgct tacaataatt cgattcgtgc acttcaaagt
     8401 gacttaacaa gtgctaaaaa tagcgctaat gctatcattc agaagccaat aagaacagtg
     8461 caagaggtac aatctgcgtt aacaaatgta aatcgtgtca atgagcgatt aacgcaagca
     8521 attaatcaat tagtaccttt agctgataat agtgctttaa gaactgctaa gacgaaactt
     8581 gatgaagaaa tcaataaatc agtaactact gatggtatga cacaatcatc aatccaagca
     8641 tatgaaaatg ctaaacgtgc aggtcaaaca gaaacaacaa atgcacaaaa tgttattaac
     8701 aatggtgacg cgacagacca acaaattgcc gcagaaaaaa caaaagtaga agaaaaatat
     8761 aatagcttaa aacaagcaat tgctggatta acaccagact tggcaccatt acaaactgca
     8821 aaaactcagt tgcaaaatga tattgatcag ccaacgagta cgactggtat gacaagcaca
     8881 tctgttgctg catttaatga caaactttca gcagctagaa ctaaaattca agaaattgat
     8941 cgcgtactag catctcatcc agatgtagca acgattcgtc aaaacgtgac agcagcgaat
     9001 gctgctaaaa cagcacttga tcaagcgcgc aatggcttaa cagtcgataa agcaccttta
     9061 gaaaatgcga aaaatcaact acaacatagt attgatacgc aaacaagtac aactggtatg
     9121 acacaagact ctataaatgc atacaatgcg aagttaacag ctgcacgtaa taaggttcaa
     9181 caaatcaatc aagtattagc aggttcacct actgtagatc aaattaatac aaatacgtct
     9241 gcagcaaatc aagcgaaatc tgatttagat catgcacgtc aagcgttaac accagataaa
     9301 gcgccgcttc aaaatgcgaa aacgcaatta gaacaaagca ttaatcaacc aacagataca
     9361 acaggtatga caaccgcttc gttaaatgca tacaaccaaa aattacaagc agcacgtcaa
     9421 aagttaactg aaattaatca agtgttgaat ggcaacccaa ctgtccaaaa tatcaatgat
     9481 aaagtggcag aggcaaacca agctaaggat caattaaata cagcacgtca aggtttaaca
     9541 ttagatagac agccagcgtt aacaacatta catggtgcat ctaacttaaa ccaagcacaa
     9601 caaaataatt tcacgcaaca aattaatgct gctcaaaatc atgctgcgct tgaaacaatt
     9661 aagtctaaca ttacggcttt aaatactgcg atgacgaaat taaaagacag tgttgcggat
     9721 aataatacaa ttaaatcagg tcaaaattac actgacgcaa caccagctaa taaacaagcc
     9781 tatgataatg cagttaatgc ggctaaaggt gtcattggag aaacgactaa tccaacgatg
     9841 gatgttaaca cagtgaacca aaaagcagca tctgttaaat cgacgaaaga tgctttagat
     9901 ggtcaacaaa acttacaacg tgcgaaaaca gaagcaacaa atgcgattac gcatgcaagt
     9961 gatttaaacc aagcacaaaa gaatgcatta acacaacaag tgaatagtgc acaaaacgtg
    10021 caagcagtaa atgatattaa acaaacgact caaagcttaa atactgctat gacaggttta
    10081 aaacgtggcg ttgctaatca taaccaagtc gtacaaagtg ataattatgt caacgcagat
    10141 actaataaga aaaatgatta caacaatgca tacaaccatg cgaatgacat tattaatggt
    10201 aatgcacaac atccagttat aacaccaagt gatgttaaca atgctttatc aaatgtcaca
    10261 agtaaagaac atgcattgaa tggtgaagct aagttaaatg ctgcgaaaca agaagcgaat
    10321 actgcattag gtcatttaaa caatttaaat aatgtacaac gtcaaaactt acaatcgcaa
    10381 attaatggtg cgcatcaaat tgatgcagtt aatacaatta agcaaaatgc aacaaacttg
    10441 aatagtgcaa tgggtaactt aagacaagct gttgcagata aagatcaagt gaaacgtaca
    10501 gaagattatg cggatgcaga tacagctaaa caaaatgcat ataacagtgc agtttcaagt
    10561 gctgaaacaa ttattaatca aacagctaat ccgacaatgt ctgttgatga tgttaatcgt
    10621 gcaacttcag ctgttactac taataaaaat gcattaaatg gtgatgaaaa attagtacaa
    10681 tctaaaacag atgctgcaag agcaattgat gcattaccac atttaaataa tgcacaaaaa
    10741 gcagatgtta aatctaaaat taatgctgca tcaaatatta ctggtgtaaa taccgttaaa
    10801 caacaaggta cagatttaaa tacagcgatg ggtaacttgc agggtgcaat caatgatgaa
    10861 caaacgacgc ttaatagtca aaattatcaa gatgcgacac ctagtaagaa aacagcatac
    10921 acaaatgcgg tgcaagctgc gaaagatatt ttaaataaat caaatggtca aaataaaacg
    10981 aaagatcaag ttactgaagc gatgaatcaa gtgaattcgg ctaaaaataa cttagatggt
    11041 acgcgtttat tagatcaagc gaagcaaaca gcgaaacagc agttaaataa tatgacgcat
    11101 ttaacaactg cacaaaaaac gaatttaaca aatcaaatta atagtggtac tactgttgct
    11161 ggtgttcata cggttcaatc aaatgccaac acattagatc aagcgatgaa tacgttaaga
    11221 caaagtattg ctaacaatga tgcgactaaa gcaagtgaag attacgtaga tgctaataat
    11281 gataagcaaa cagcatataa caacgcggta gctgctgctg aaacgattat taatgcgaat
    11341 agtaatccag aaatgaatcc aagtacgatt acacaaaaag cagagcaagt gaatagttct
    11401 aaaacggcac ttaacggtga tgaaaactta gctacggcaa aacaaaatgc gaaaacgtac
    11461 ttaaacacat taacgagtat tacagatgct caaaagaaca atttgattag tcaaattagt
    11521 agtgcgacaa gagtgagtgg tgttgatact gtaaaacaaa atgcacaaca tttagatcaa
    11581 gctatggcta acttacaaaa tggtattaac aacgaatctc aagtgaaatc atctgagaaa
    11641 tatcgtgatg ctgatacaaa taaacaacaa gagtatgata atgctattac tgcagcgaaa
    11701 gcgattttaa ataaatcgac aggtccaaac actgcgcaaa atgcagttga agcagcattg
    11761 caacgtgtta atactgcgaa agatgcattg aatggtgatg caaaattaat tgcagctcaa
    11821 aacgcagcga aacaacattt aggtacttta acgcatatca ctacagcaca acgcaatgat
    11881 ttaacaaatc aaatttcaca agctacaaac ttagctggtg ttgaatcagt taaacaaagt
    11941 gcaaatagtt tagatggcgc aatgggtaac ttacaaacgg ctatcaacga taagtcagga
    12001 acattagcga gccaaaactt cttggatgct gatgagcaaa aacgtaatgc ttacaatcaa
    12061 gctatatcag ctgccgaaac cattttaaat aaacaaactg gaccgaatac agcgaaaaca
    12121 gcggttgaac aagcacttaa taatgttaat agtgcgaaac atgcattaaa tggtacgcaa
    12181 aacttaaata atgcgaaaca agcagcgatt acagcaatta atggcgcatc tgatttaaat
    12241 caaaaacaaa aagatgcatt aaaagcacaa gctaatggtg ctcaacgcgt atctaatgca
    12301 aatgatgtac aacgtaatgc gactgaactg aacacggcaa tgggtcaatt acaacatgcc
    12361 atcgcagata agacgaatac gttagcaagc agtaaatatg tcaacgccga tagcactaaa
    12421 caaaatgctt acacaactaa agttaccaat gctgaacata ttattagcgg tacgccaacg
    12481 gttgttacaa caccttcaga agtaacagct gcagctaatc aagtaaacag cgcgaaacaa
    12541 gaattaaatg gtgacgaaag attacgtgtt gcaaaacaaa acgccaatac tgctattgat
    12601 gcattaacgc aattaaatac tcctcaaaaa gctaaattaa aagaacaagt gggacaagcc
    12661 aatagattag aagacgtaca atctgttcaa acaaatggac aatcattgaa caatgcaatg
    12721 aaaggcttaa gagatagtat tgctaacgaa acaacagtca aagcaagtca aaactataca
    12781 gacgcaagtc cgaataacca atcaacatat aatagcgctg tgtcaaatgc gaaaggtatc
    12841 attaatcaaa ctaacaatcc aactatggat actagtgcga ttacccaagc tacaacacaa
    12901 gtgaataatg ctaaaaatgg tttaaacggt gctgaaaact taagaaatgc acaaaacact
    12961 gctaagcaaa acttaaatac gttatcacac ttaacaaata accaaaaatc tgcaatctca
    13021 tcacaaattg atcgtgcagg tcatgtgagt gaggtaacag ctgctaaaaa tgcagcaact
    13081 gagttaaacg cgcaaatggg caacttggaa caagctatcc atgatcaaaa cacagttaaa
    13141 caaggtgtta acttcactga tgcagataaa gctaaacgtg atgcttatac aaatgcggta
    13201 agcagagcag aaacaattct gaataaaacg caaggtgcaa atacgtctaa acaagatgtt
    13261 gaagcggcta ttcaaaatgt tacaagtgct aaaaatgcat tgaatggtga tcaaaacgtt
    13321 acaaatgcga agaatgcagc taaaaatgca ttaaataact taacgtcaat taataatgca
    13381 caaaaacgtg acttaacaac taaaattgat caagcaacaa cagtagctgg tgttgaagcg
    13441 gtatctaata caggtacaca attgaataca gcgatggcta acttgcaaaa tggtattaat
    13501 gataaagcga atactttagc gagcgaaaac tatcatgatg ctgattcaga taagaaaact
    13561 gcttatactc aagccgttac gaacgcagaa aatattttaa ataaaaatag tggatcaaat
    13621 ttagataaag ctgccgttga aaacgcgttg tcacaagtga caaatgcgaa aggtgcccta
    13681 aatggtaacc ataatttaga gcaagctaaa tcaaatgcaa acactactat aaacggcctt
    13741 caacatttaa caacagcaca aaaagataaa ttgaaacaac aagtgcaaca agcacaaaat
    13801 gttgcaggtg tagatactgt taaatcaagt gccaacacat taaatggtgc tatgggtacg
    13861 ttaagaaata gcatacaaga taacacagct acgaaaaatg gccaaaacta tcttgatgct
    13921 acagaacgta acaaaacaaa ctataacaat gctgttgata gtgctaatgg tgtcattaat
    13981 gcaacaagca atccaaatat ggatgctaat gcaattaacc aaatcgctac acaagtgaca
    14041 tcaacgaaaa atgcattaga tggtacacat aatttaacgc aagcgaaaca aacagcaaca
    14101 aatgccatcg atggtgctac taacttaaat aaagcgcaaa aagatgcgtt aaaagcacaa
    14161 gttacaagtg cgcaacgtgt tgcaaatgta acaagtatcc aacaaactgc aaatgaactt
    14221 aatacagcta tgggtcaatt acaacatggt attgatgatg aaaatgcaac aaaacaaact
    14281 caaaaatatc gtgacgctga acaaagtaag aaaactgctt atgatcaagc tgtagctgct
    14341 gcgaaagcaa ttttaaataa acaaacaggt tccaattcag ataaagcagc agttgaccgt
    14401 gcattacaac aagtaacaag tacgaaagat gcattgaatg gggatgctaa actggcagaa
    14461 gcgaaagcgg cagctagaca aaacttaggt actttaaacc atattacgaa tgcacaacgt
    14521 actgcgttag aaggtcaaat caatcaagcg acgactgttg atggcgttaa tactgtaaaa
    14581 acaaatgcca atacattaga cggcgctatg aatagcttac aaggtgcaat caatgataaa
    14641 gatgcgacat taagaaatca aaattatctt gatgcagatg aatcaaaacg aaatgcatat
    14701 acgcaagctg tcacagcggc tgaaggcatt ttaaataaac aaacaggtgg taacacatct
    14761 aaagcagacg ttgataatgc attaaatgca gttacaagag cgaaagcggc tttaaatggt
    14821 gctgaaaact taagaaatgc gaaaacttca gcaacaaata cgattaatgg tttacctaac
    14881 ttaacacaat tacaaaaaga caacttgaag catcaagttg aacaagcgca aaatgtagtt
    14941 ggtgtaaatg gtgttaaaga taaaggtaat acattaaata ctgccatggg tgcattacgt
    15001 acaagtatcc aaaatgataa tacgacgaaa acaagtcaaa attatcttga tgcatctgat
    15061 agcaacaaaa ataattacaa tactgctgta aataatgcaa atggtgttat taatgcaacg
    15121 aacaatccaa atatggatgc taatgcgatt aatgacatgg caaatcaagt caatacaaca
    15181 aaagcagcgt taaatggtgc acaaaactta gctcaagcta aaacaaatgc gacgaacaca
    15241 attaacaacg cgcaagactt aaaccaaaaa caaaaagatg cattaaaaac acaagttaac
    15301 aatgcacaac gtgtatctga tgcaaataac gttcaacata cagctactga attgaacggt
    15361 gcgatgacag cacttaaagc agctattgcg gataaagaaa gaacaaaagc aagcggtaat
    15421 tatgtcaatg ctgatcaaga aaaacgtcaa gcgtatgatt caaaagtgac taacgctgaa
    15481 aatatcatta atggtacacc aaatgcgaca ttaacagtca atgacgtaaa tagtgcggca
    15541 tcacaagtca atgcggctaa aacagcatta aatggtgata acaacttacg tgtagcgaaa
    15601 gagcatgcta acaatacaat tgacggctta gcacaattga ataatgtaca aaaagcaaaa
    15661 ttaaaagaac aagttcaaag tgcaactaca ttagatggtg ttcaaactgt taaaaatagt
    15721 tctcaaacgt tgaatacagc gatgaaaggc ttaagagata gtattgcgaa tgaagcaacg
    15781 attaaagcag gtcaaaacta cactgacgca agtccaaata atcgtaacga gtacgacagc
    15841 gcagttactg cagcaaaagc aatcattaat caaacatcga acccaacgat ggaaccaaat
    15901 actattacgc aagcaacatc acaagtgaca actaaagaac atgcattaaa tggtgcgcaa
    15961 aacttagctc aagctaagac aacagcgaaa aacaacttga ataacttaac atcaattaac
    16021 aatgcacaaa aagatgcgtt aacgcgtaac attgatggtg caactacagt agctggtgta
    16081 aatcaagaaa ctgcaaaagc aacagaatta aataacgcaa tgcacagttt acaaaatggt
    16141 atcaatgatg agacacaaac aaaacaaact cagaaatacc tagatgctga gccaagtaag
    16201 aaatcagctt atgatcaagc agtaaatgca gcaaaagcaa ttttaacaaa agctagtggt
    16261 caaaatgtag acaaagcagc agttgaacaa gcattacaaa atgtgaacag tacgaagacg
    16321 gcgttgaacg gtgatgcgaa attaaatgaa gctaaagctg ctgcgaaaca aacgttaggt
    16381 acattaacac acattaataa tgcacaacgt aatgcgttag ataatgaaat tacacaagca
    16441 acaaatgttg aaggtgttaa tacagttaaa gccaaagcgc aacaattaga tggtgctatg
    16501 ggtcaattag aaacatcaat tcgtgataaa gacacgacgt tacaaagtca aaattatcaa
    16561 gatgctgatg atgctaaacg aacggcttat tctcaagcag taaatgcagc agcaactatt
    16621 ttaaataaaa cagctggagg aaatacacct aaagcagatg tcgaaagagc aatgcaagct
    16681 gttacacaag ccaatactgc attaaacggt attcaaaact tagaacgtgc gaaacaggct
    16741 gcgaacacag cgattacaaa tgcttcggac ttaaatacaa aacaaaaaga agcattgaaa
    16801 gcacaagtaa caagtgcagg acgcgtatct gcagcaaatg gtgttgaaca tactgcgact
    16861 gaattaaata ctgcgatgac agctttaaaa cgtgccattg ctgataaagc tgacacaaaa
    16921 gctagtggta attatgtcaa tgctgatgcg aataaacgcc aagcatatga tgaaaaagtg
    16981 acagctgcag aacatatcgt tagtggtaca ccaacaccaa cgttaacacc atcagatgtt
    17041 acaaatgcag caacgcaagt aacgaatgcg aagacgcagt taaacggtaa tcataattta
    17101 gaagtagcga aacaaaatgc taacacagca attgatggtt taacttcttt aaatggtccg
    17161 caaaaagcaa aacttaaaga acaagtgggt caagcgacga cgttgccaaa tgttcaaact
    17221 gttcgtgata atgcacaaac attaaacact gcaatgaaag gtctacgaga tagcattgcg
    17281 aatgaagcaa cgattaaagc aggtcaaaac tacacagatg caagtcaaaa caaacaaaat
    17341 gactacaaca atgcagtcac tgcagcaaaa gcaatcattg gtcaaacaac tagtccatca
    17401 atgattgcgc aagaaattaa tcaagcgaaa gaccaagtga cagctaaaca acaagcgtta
    17461 aacggtcaag aaaacttaag aactgcgcaa acaaatgcga agcaacattt gaatggctta
    17521 agtgacttaa ctaatgcaca aaaagatgca gcgaaacgcc aaatcgaagg tgcaacgcat
    17581 gttaatgaag taacacaagc gcaaaataat gcggacgcat taaatacagc tatgacgaac
    17641 ttgaaaaatg gtattcaaga tcaaaatacg attaagcaag gtgttaactt cactgatgca
    17701 gatgaagcga aacgtaatgc atatacaaat gcagtgacgc aagctgaaca aattttaaat
    17761 aaagcacaag gtccaaatac tgcaaaagac ggtgtcgaaa ctgcgttaca aaatgtacaa
    17821 cgtgctaaaa acgaattgaa cggtaatcaa aatgttgcga acgctaagac aactgcgaaa
    17881 aatgcattga ataaccttac atcaattaat aatgcacaaa aagcagcatt gaaatcacaa
    17941 attgaaggtg cgacaacagt tgcaggtgta aatcaagtgt ctacaatggc atctgaatta
    18001 aatactgcaa tgagcaactt acaacgtggt attaatgacg aagcagctac aaaagcagct
    18061 cagaaatata ctgaagcaga tagagataaa caaactgcat acaatgatgc tgtaacagca
    18121 gctaaaacgt tattagataa aacagctggt tcaaatgaca ataaagtagc cgttgaacaa
    18181 gcattacaac gtgtgaatac tgctaaaaca gcattaaatg gtgacgcgcg attaaatgaa
    18241 gcgaagaaca cagctaaaca acaattagcg acaatgtcac atttaactaa tgctcaaaaa
    18301 gcaaacttaa cagaacaaat tgaacgtggt acaactgttg ctggtgttca aggcatccaa
    18361 gcaaatgctg gtactttaaa tcaagcaatg aatcaattaa gacaaagtat tgcttctaaa
    18421 gatgcgacta aatcaagcga agattatcaa gacgcgaatg cagatttaca aaatgcatac
    18481 aatgatgcgg taactaatgc tgaaggtatt attagtgcaa cgaataaccc tgaaatgaat
    18541 cctgatacaa ttaaccaaaa agcgagccaa gtgaacagtg cgaagtctgc attgaacggt
    18601 gatgaaaaat tagcagcagc aaaacaaact gcgaaatcag atatcggtcg tttgacagac
    18661 ttgaacaatg cacaacgaac tgcggcaaat gctgaagtgg atcaagcacc aaatcttgca
    18721 gctgtcacag cggctaaaaa taaagcaaca tcgttaaaca cagcgatggg taatttgaaa
    18781 catgcacttg ctgaaaagga taatacgaaa cgtagtgtca attacacaga tgcggatcaa
    18841 ccaaaacaac aagcgtatga tactgcagtt acacaagcag aagcaattac taatgcaaat
    18901 ggcagtaacg cgaatgaaac acaagttcaa gcagcgctta accaattgaa tcaagctaaa
    18961 aacgacttga atggtgataa taaagttgct caagcgaaag aaacagcaaa acgtgcatta
    19021 gcttcatata gtaacttgaa taacgcgcaa tcaactgcag caactagtca aattgacaat
    19081 gcaacgacag tagcagacgt aactgctgca caaaatactg ctaatgaatt aaatacagca
    19141 atgggtcaac ttcaaaatgg tattaatgac caaaacactg ttaaacaaca agtgaacttt
    19201 acagatgctg accaaggtaa gaaagatgct tacacaaatg ctgttacgaa tgctcaaggt
    19261 attttagata aagcaaacgg tcaaaatatg acaaaagcac aagttgaagc tgcattaaat
    19321 caagtaacga ctgctaagaa tgctttaaac ggtgatgcaa atgtaagaca agcaaaatca
    19381 gatgcgaaag caaacttagg tacattaaca cacttaaata atgcacaaaa acaagattta
    19441 acatcacaaa tcgaaggtgc aacaacagtc aacggtgtaa atagtgttaa aacgaaagca
    19501 caagacttag atggtgcaat gcaacgatta gagtcagcaa tcgcaaataa agatcaaact
    19561 aaagcgagcg aaaacaacat tgacgcagat ccaactaaga aaacagcatt tgataatgcc
    19621 atcacacaag ctgaatctta cttaaataaa gatcatggta cgaataaaga taagcaagct
    19681 gttgaacaag caattcaaag tgtaacgtct actgaaaatg ctttgaacgg tgacgcgaac
    19741 ttacaatgcg ctaaaactga agctacacaa gctatcgata acttgacaca attgaataca
    19801 ccgcaaaaaa cagcattgaa acaacaagtg aatgctgcac aacgcgtatc aggtgtaact
    19861 gatctgaaaa atagtgctac atcacttaat aatgcgatgg atcaattaaa acaagcaatt
    19921 ggtgatcatg acacaattgt agctggtggt aattacacta acgcaagtcc tgataaacaa
    19981 ggtgcttaca ctgatgcata taatgctgcg aagaatatcg taaatggttc acctaatgtg
    20041 attacaaatg cagcagatgt tactgcggca acacaacgtg tcaataatgc tgaaacaagt
    20101 ttaaatggtg atacaaactt agcaactgcg aagcaacaag ctaaagatgc attacgtcaa
    20161 atgacacatt tatctgatgc acaaaaacaa agtattactg gtcaaattga tagcgcgaca
    20221 caagtaactg gtgtacaaag tgtgaaagac aatgcaacaa atcttgacaa tgcaatgaat
    20281 caacttcgaa atagtattgc gaataaagat gaagtaaaag cgagtcaacc atatgttgat
    20341 gcagatacag ataaacaaaa tgcatacaat acagcagtta caagtgctga aaatatcatt
    20401 aatgcaacga gtcagccaac acttgatcca tctgcagtaa cacaagcagc taatcaagtg
    20461 aacactaaca aaactgcgct taatggtgcg caaaacttag caaataaaaa gcaagaaaca
    20521 actgctaaca tcaaccgatt aagtcattta aacaatgctc aaaagcaaga tttaaataca
    20581 caagtgacaa atgcaccaaa tattagcaca gtaaatcaag tgaaaactaa agctgaacaa
    20641 ttagatcaag caatggaacg tttaatcaac ggaatccaag acaaagatca agtgaaacaa
    20701 agtgttaact ttacagatgc agatccagaa aaacaaacag catacaacaa tgcggtaact
    20761 gctgctgaaa atattattaa tcaagcaaat ggtacaaatg cgaaccaatc acaagttgaa
    20821 gcagcacttt caactgtaac aactactaaa caagcgttga atggtgatag aaaagtaaca
    20881 gatgctaaaa acaatgcaaa ccaaacatta tctacgttag ataacttaaa caatgcacaa
    20941 aaaggtgctg ttactggaaa catcaatcaa gcgcacactg tagctgaagt aacgcaagcc
    21001 attcaaaccg ctcaggaact gaatacagcg atgggtaact tgaaaaatag cttgaatgat
    21061 aaagacacta cacttggcag tcaaaacttt gcagatgcag atccagagaa gaaaaatgca
    21121 tacaatgaag cggttcgtaa tgctgaaaat attttaaata aatctacagg tacgaacgtg
    21181 cctaaagatc aagttgaagc agctatgaat caagtgaata ctacaaaagc agcgcttaat
    21241 ggtactcaaa accttgaaaa agcgaaacaa cacgcaaata cagcaattga cggtttaagc
    21301 catttaacaa atgcacaaaa agaggcatta aaacaattgg tacaacaatc gactactgtt
    21361 gcagaagcac aaggtaatga acaaaaagca aacaatgttg atgcagcaat ggacaaatta
    21421 cgtcaaagta ttgcagataa tgcgacaaca aaacaaaacc aaaattatac tgatgcaagt
    21481 ccgaataaaa aggatgcgta caataatgct gtcacaactg cacaaggtat tattgatcaa
    21541 actacaaacc cttcattaga tccgactgtt atcaatcaag ctgctggaca agtaagcacg
    21601 tctaaaaatg ctttaaatgg taatgaaaac ttagaggcag cgaagcaaca agcaacgcaa
    21661 tctttaggtt cattagacaa cttaaataat gcgcaaaaac aagctgttac taatcaaatt
    21721 aatggcgcgc atactgttga tgaagcaaat caaattaagc aaaatgcgca aaacttaaat
    21781 actgcgatgg gtaacttgaa acaagcgata gctgataaag atgctacgaa agcaacagtt
    21841 aacttcactg atgcagatca agcaaaacaa caagcatata acactgcagt tacaaatgct
    21901 gaaaatatca tttcaaaagc taatggtggt aatgcaacac aaactgaagt tgaacaagca
    21961 atccaacaag taaatgcagc aaaacaagca ttaaatggta atgccaacgt tcaacatgca
    22021 aaagacgaag caacagcatt aattaataac tctaatgatc ttaaccaagc acagaaagat
    22081 gcattaaaac aacaagtaca aaatgcaact actgtagctg gtgtaaacaa tgttaaacaa
    22141 acggcgcaag agttaaacaa tgcgatgaca caattaaaac aaggcattgc agataaagaa
    22201 caaacaaaag ctgatggtaa ctttgtcaat gcagattctg acaagcaaaa tgcatataat
    22261 caagcagtag cgaaagctga agcattaatt agtggtacgc ctgatgttgt cgttacacct
    22321 agcgaaatta ctgcagcgtt aaataaagtt acgcaagcta aaaatgattt aaatggtaat
    22381 acaaacttag caacggcgaa acaaaatgtt caacatgcta ttgatcaatt gccaaactta
    22441 aaccaagcgc aacgtgatga atacagcaaa caaatcacgc aagcaacact tgtaccaaac
    22501 gtcaatgcta ttcaacaagc ggcaacaacg cttaatgacg cgatgacaca attgaaacaa
    22561 ggtattgcga ataaagcaca aattaaaggt agcgagaact atcacgatgc tgatactgac
    22621 aagcaaacag catatgataa tgcagtaaca aaagcagaag aattgttaaa acaaacaaca
    22681 aatccaacaa tggatccaaa tacaattcaa caagcattaa ctaaagtgaa tgacacaaat
    22741 caagcactta acggtaatca aaaattagct gatgccaaac aagatgctaa gacaacactt
    22801 ggtacactag atcatttaaa tgatgctcaa aaacaagcgc taacaactca agttgaacaa
    22861 gcaccagata ttgcaacagt taataatgtt aagcaaaatg ctcaaaatct gaataatgct
    22921 atgactaact taaacaatgc attacaagat aaaactgaga cattaaatag cattaacttt
    22981 actgatgcag atcaagctaa gaaagatgat tatactaatg cggtttcaca tgcagaaggt
    23041 attttatcta aagcaaatgg cagcaatgca agtcaaactg aagtggaaca agcgatgcaa
    23101 cgtgtgaacg aagcgaaaca agcattgaat ggtaatgaca atgtacaacg tgcaaaagat
    23161 gcagcgaaac aagtaattac aaatgcaaat gatttaaatc aagcgcaaaa agatgcatta
    23221 aaacaacaag tcgatgctgc gcaaactgtt gcaaatgtaa acacgattaa gcaaacagca
    23281 caagatttaa atcaagcaat gacacaattg aaacaaggta ttgcagataa agaccaaact
    23341 aaagcaaatg gtaactttgt caatgctgat actgataagc aaaatgcata taacaatgcg
    23401 gtagcgcatg ctgaacaaat cattagtggt acaccaaatg caaacgtgga tccacaacaa
    23461 gtggctcaag cgttacaaca agtgaatcaa gctaagggtg atttaaacgg taaccataac
    23521 ttacaagttg ctaaagacaa tgcaaataca gccattgatc agttaccaaa cttaaatcaa
    23581 ccacaaaaaa cagcattaaa agaccaagtg tcgcatgcag aacttgttac aggtgttaat
    23641 gctattaagc aaaatgctga tgcgttaaat aatgcaatgg gtacgttgaa acaacaaatt
    23701 caagcgaata gtcaagtacc acaatcagtt gactttacac aagcggatca agacaaacaa
    23761 caagcttata acaatgcagc taaccaagcg caacaaatcg caaatggcac accaacacct
    23821 gtattggcgc ctgatacagt aacaaaagca gttacaacta tgaatcaagc gaaagatgca
    23881 ttaaacggtg atgaaaaatt agcgcaagcg aaacaagatg ctttagcaaa tcttgatacg
    23941 ttacgtgact taaatcaacc acaacgtgat gcattacgaa accaaatcaa tcaagcacaa
    24001 gctttagcta cagttgaaca aactaaacaa aatgcacaaa atgtgaatac agcaatgggt
    24061 aacttgaaac aaggtattgc aaataaagat actgtgaaag caagtgagaa ctaccacgat
    24121 gctgatgtcg ataagcaaac agcatataca aatgcagtgt ctcaagcgga aggtattatc
    24181 aatcaaacga caaatccaac gcttaaccca gatgacatta ctcgtgcatt aactcaagtg
    24241 actgatgcta aaaatagctt aaacggtgaa gctaaattag ccactgaaaa gcaaaatgct
    24301 aaagatgccg taagtggaat gacgcattta aacgatgctc aaaaacaagc attaaaaggt
    24361 caaatcgatc aatcgcctga aattgctaca gtgaaccaag ttaaacaaac agcaacgagc
    24421 ctagatcaag caatggatca attatcacaa gctattaatg ataaagatca aatattagcg
    24481 gacggtaatt acttaaatgc agatcctgac aaacaaaatg cgtataaaca ggcagtagca
    24541 aaagctgaag cattattgaa taaacaaagt ggtactaatg aagtacaagc acaagttgaa
    24601 agcatcacta atgaagtgaa cgcagcgaaa caagcattaa atggtaatga caatttggca
    24661 aatgcaaaac aacaagcaaa acaacaattg gcgaacttaa cacacttaaa tgatgcacaa
    24721 aaacaatcat ttgaaagtca aattacacaa gcgccacttg ttacagatgt cactacgatt
    24781 aatcaaaaag cacaaacgtt agatcatgcg atggaattat taagaaatag tgttgcggat
    24841 aatcaaacga cattagcgtc tgaagattat catgatgcaa ctgcgcaaag acaaaatgac
    24901 tataacaaag ctgtaacagc tgctaataat atcattaatc aaactacatc gcctacgatg
    24961 aatccagatg atgttaatgg tgcaacgaca caagtgaata atacgaaagt tgcattagat
    25021 ggtgatgaaa accttgcagc agctaaacaa caagcaaaca acagacttga tcaattagat
    25081 catttgaata atgcgcaaaa gcaacagtta caatcacaaa ttacgcaatc atctgatatt
    25141 gctgcagtta atggtcacaa acaaacagca gaatctttaa atactgcgat gggtaactta
    25201 attaatgcga ttgcagatca tcaagccgtt gaacaacgtg gtaacttcat caatgctgat
    25261 actgataaac aaactgctta taatacagcg gtaaatgaag cagcagcaat gattaacaaa
    25321 caaactggtc aaaatgcgaa ccaaacagaa gtagaacaag ctattactaa agttcaaaca
    25381 acacttcaag cgttaaatgg agatcataat ttacaagttg ctaaaacaaa tgcgacgcaa
    25441 gcaattgatg ttttaacaag cttaaatgat cctcaaaaaa cagcattaaa agaccaagtt
    25501 acagctgcaa ctttagtaac tgcagttcat caaattgaac aaaatgcgaa tacgcttaac
    25561 caagcaatgc atggtttaag acagagcatt caagataacg cagcaactaa agcaaatagc
    25621 aaatatatca acgaagatca accagagcaa caaaactatg atcaagctgt tcaagccgca
    25681 aataatatta tcaatgaaca aactgcaaca ttagataata atgcgattaa tcaagtagcg
    25741 gcaactgtga atacaacgaa agcagcatta catggtgatg tgaaattaca aaatgataaa
    25801 gatcatgcta aacaaacggt tagccaatta gcacatctaa acaatgcaca aaaacatatg
    25861 gaagatacgt taattgatag tgaaacaact agaacagcag ttaagcaaga tttgactgaa
    25921 gtacaagcat tagatcaact tatggatgca ttacaacaaa gtattgctga caaagatgca
    25981 acacgtgcga gcagtgcata tgtcaatgca gaaccgaata aaaaacaagc ctatgatgaa
    26041 gcagttcaaa atgctgagtc tatcattgca ggattaaata atccaactat caataaaggt
    26101 aatgtatcaa gtgcgactca agcagtaata tcatctaaaa atgcattaga tggtgttgaa
    26161 cgattagctc aagataagca aactgctgga aattctctaa atcatttaga tcaattaaca
    26221 ccagctcaac aacaagcgct agaaaatcaa attaataatg caacaactcg tgataaagtg
    26281 gctgaaatca ttgcacaagc gcaagcatta aatgaagcga tgaaagcatt aaaagaaagt
    26341 attaaggatc aaccacaaac tgaagcaagt agtaaattta ttaacgagga tcaagcgcaa
    26401 aaagatgcat atacgcaagc agtacaacac gcgaaagatt tgattaacaa aacaactgat
    26461 cctacattag ctaaatcaat cattgatcaa gcgacacagg cagtgactga tgctaaaaac
    26521 aatttacatg gtgatcaaaa actagctcaa gataagcaac gtgcaacaga aacgttaaat
    26581 aacttgtcta acttgaatac accacaacgt caagcacttg aaaatcaaat caataatgca
    26641 gcaactcgtg gtgaagtagc acaaaaatta actgaagcac aagcacttaa ccaagcaatg
    26701 gaagctttac gtaatagcat tcaagatcaa caacaaacag aatctggtag caagtttatt
    26761 aatgaagata aaccgcaaaa agatgcttac caagcagcag ttcaaaatgc aaaagattta
    26821 attaaccaaa caggtaatcc aacgcttgat aaagcacaag ttgaacaatt gacacatgct
    26881 tttaaacaag ctaaagataa cctacacggt gatcaaaaac ttgcagacga taaacaacat
    26941 gcggttactg atttaaatca attaaatggt ttgaataatc cgcaacgtca agcacttgaa
    27001 agccaaataa acaacgcagc aactcgtggc gaagtagcgc aaaaattagc tgaagcaaaa
    27061 gcgcttgatc aagcaatgca agcattacga aatagtattc aagatcaaca acaaacggaa
    27121 gcgggtagca agtttatcaa tgaagataaa ccgcaaaaag atgcttacca agcagcagtt
    27181 caaaatgcaa aagatttaat taaccaaaca ggtaatccaa cactcgacaa atcacaagta
    27241 gaacaattaa cacaagcagt aacaactgca aaagataatc tacatggtga tcaaaaactt
    27301 gctcgtgatc aacaacaagc agtaacaact gtaaatgcat tgccaacctt aaatcatgca
    27361 caacaacaaa cattaactga tgctataaat gcagcgccta caagaacaga ggttgcacaa
    27421 catgttcaaa ctgctactga acttgatcac gcgatggaaa cattgaaaaa taaagttgat
    27481 caagtgaata cagataaggc tcaaccaaat tacactgaag cgtcaactga taaaaaagaa
    27541 gcagtagatc aagcgttaca agctgcacaa agcattacag atccaactaa tggttcaaat
    27601 gcgaataaag acgctgtaga acaagcatta actaagcttc aagaaaaagt gaatgagtta
    27661 aatggtaatg agagagtcgc tgaagctaaa acacaagcga aacaaactat tgaccaatta
    27721 acacatttaa atgctgatca aattgcaact gctaaacaaa atattgatca agcgacgaaa
    27781 cttcaaccaa tcgctgaatt agtagatcaa gcaacgcaat tgaaccaatc aatggatcaa
    27841 ttacaacaag cagttaatga acatgctaac gttgagcaaa ctatagatta cacacaagca
    27901 gattcagata agcaaaaggc ttataaacaa gcgattgctg atgctgaaaa tgtattgaaa
    27961 caaaatgcga ataagcaaca agtggatcaa gcacttcaaa atattttaaa tgcaaaacaa
    28021 gcattaaatg gtgatgaacg tgtagcactt gctaaaacaa atggtaaaca tgacatcgac
    28081 caattgaatg cattaaacaa tgctcaacaa gatggattta aaggtcgcat cgatcaatca
    28141 aacgatttaa atcaaatcca acaaattgta gatgaggcta aggcacttaa tcgtgcaatg
    28201 gatcaattgt cacaagaaat cactggcaat gaaggacgca cgaaaggtag cacgaactat
    28261 gtcaatgcag atacacaagt caaacaagta tatgatgaag cggttgataa agcgaaacaa
    28321 gcacttgata aatcgtctgg gcaaaactta actgcagaac aagttatcaa attaaatgat
    28381 gcagtcactg cagctaagaa agcattaaat ggtgaagaaa gacttaataa tcgtaaagct
    28441 gaagcattac aaagattgga tcaattaaca catctaaaca atgctcaaag acaattagca
    28501 atccaacaaa ttaataatgc tgaaacgcta aataaagcat ctcgagcaat taatagagca
    28561 actaaattag ataatgcaat gggtgcagta caacaatata ttgacgaaca gcaccttggt
    28621 gttatcagca gcacaaatta catcaatgca gatgacaatt tgaaagcaaa ttatgataat
    28681 gcaattgcga atgcagcaca tgagttagat aaagtgcaag gtaatgcaat tgcaaaagct
    28741 gaagcagagc aattgaaaca aaatattatc gatgctcaaa atgcattaaa tggagaccaa
    28801 aaccttgcaa atgccaaaga taaagcaaat gcgtttgtta attcgttaaa tggattaaat
    28861 caacagcaac aagatcttgc acataaagca attaacaatg ccgatactgt atcagatgta
    28921 acagatattg ttaataatca aattgactta aatgatgcaa tggaaacatt gaaacattta
    28981 gttgacaatg aaattccaaa tgcagagcaa actgtcaatt accaaaacgc tgacgataat
    29041 gctaaaacaa acttcgatga tgccaaacgt ctagcaaata cattgctaaa tagtgataac
    29101 acaaatgtga atgatatcaa tggcgcaatc caagcagtca atgatgcaat ccataatctt
    29161 aatggtgatc aacgactaca agatgctaaa gacaaggcaa ttcaatcaat taatcaagct
    29221 ttagctaata agctaaaaga aatcgaagct tcaaatgcga cggatcaaga caagcttatt
    29281 gcgaaaaata aagcagaaga attggcaaac agcatcatca acaacattaa taaagcaaca
    29341 agtaatcagg ctgtatctca agttcaaaca gcaggcaacc acgcgattga acaagtgcat
    29401 gctaatgaaa taccaaaagc aaaaattgat gccaataaag acgttgataa gcaagttcaa
    29461 gcattaattg acgaaattga tcgaaatcca aatctaacag ataaggaaaa acaagcactt
    29521 aaagatcgta ttaatcaaat acttcaacaa ggtcataacg acattaacaa tgcgctgact
    29581 aaagaagaaa ttgaacaagc taaagcacaa cttgcgcaag cattacaaga catcaaagat
    29641 ttagtgaaag ctaaagaaga tgcgaaacaa gatgttgata aacaagttca agcattaatt
    29701 gacgaaatcg atcaaaatcc aaatctaaca gataaggaaa aacaagcact taaagatcgt
    29761 attaatcaaa tacttcaaca aggtcataac ggcattaaca atgcgatgac taaagaagaa
    29821 attgaacaag ccaaagcaca acttgcacaa gcattaaaag aaattaaaga tttagtgaaa
    29881 gctaaagaaa atgcgaaaca agatgttgat aaacaagttc aagcattaat tgacgaaatc
    29941 gatcaaaatc caaatctaac agataaggaa aaacaagcgc ttaaagatcg aatcaatcaa
    30001 atactgcaac aaggtcataa cgacattaac aatgcgatga ctaaagaaga aattgaacaa
    30061 gccaaagcac aacttgcaca agcattacaa gacatcaaag atttagtgaa agctaaagaa
    30121 gatgcgaaaa atgcaataaa agccttagct aatgcgaagc gtgatcaaat caattcaaat
    30181 ccagatttaa cacctgagca aaaagcaaaa gcgctcaaag aaattgacga agctgaaaaa
    30241 cgagcactac aaaacgttga gaatgctcaa actatagatc aattaaatcg aggattaaac
    30301 ttaggtttag atgacattag aaatacacat gtatgggagg ttgatgaaca acctgctgta
    30361 aatgaaattt ttgaagcaac acctgagcaa atcctagtta atggtgaact cattgtacat
    30421 cgtgatgaca tcattacaga acaagatatt cttgcacaca taaacttaat tgatcagctt
    30481 tcagcagaag ttattgatac accatcaact gcaacgattt ctgatagctt aacagcaaaa
    30541 gttgaagtta cattgcttga tggatcaaaa gtgattgtta atgttcctgt aaaagttgta
    30601 gaaaaagaat tgtcagtagt caaacaacag gcaattgaat caatcgaaaa tgcggcacaa
    30661 caaaagattg atgaaatcaa taatagtgtg acattaacac tggaacaaaa agaagctgca
    30721 attgcagaag ttaataagct taaacaacaa gcaattgatc atgttaacaa tgcacctgat
    30781 gttcattcag ttgaagaaat tcaacaacaa gaacaagcgt atattgaaca atttaatcca
    30841 gaacaattta cgattgaaca agcaaaatca aatgcaatta aatcgattga agatgcaatt
    30901 caacatatga ttgatgaaat caaagctcgt actgatctaa cagataaaga gaagcaagaa
    30961 gctattgcta agttaaatca attaaaagaa caagcaattc aagcgattca acgtgcgcaa
    31021 agcatcagtg aaataactga gcaattggaa caatttaaag ctcaaatgaa agcagctaat
    31081 ccaacagcaa aagaactagc taaacgcaag caagaagcta ttagtagaat taaagacttt
    31141 tcaaatgaaa aaataaatag tattcgaaat agtgaaattg gcacagctga tgaaaaacaa
    31201 gcagcaatga atcaaattaa cgaaattgtg cttgaaacaa ttagagatat taataatgcg
    31261 catacattac agcaagttga ggctgcattg aacaatggta ttgctcgaat ttcagcagta
    31321 caaattgtaa tatctgatcg tgctaaacaa tcgtcaagta ctggaaatga atctaatagc
    31381 catttaacaa ttggttatgg aactgcaaat catccattta acagttcgac tattggacat
    31441 aaaaagaaac ttgatgaaga tgatgacatt gatccacttc atatgcgtca ctttagtaat
    31501 aatttcggta atgttattaa aaacgctatt ggtgtggtgg gtatctctgg cttactagct
    31561 agtttctggt tcttcattgc caaacgtcgt cgtaaagaag atgaagagga agaattagaa
    31621 ataagagata ataataaaga ttcaataaaa gagactttag acgatacaaa acatttacca
    31681 cttttatttg cgaaacgtcg cagaaaagaa gatgaagaag atgttactgt tgaagaaaaa
    31741 gattcgctaa ataatggcga gtcactcgat aaagttaaac atacgccgtt cttcttacca
    31801 aaacgtcgtc gtaaagaaga tgaagaagat gtggaagtta caaatgaaaa cacagatgaa
    31861 aaagtgttga aagataacga acattcacca ctcttattcg caaaacgacg caaagataaa
    31921 gaggaagatg ttgaaacaac aactagtatt gaatctaaag atgaggacgt tcctttatta
    31981 ttggctaaaa agaaaaatca aaaagataac caatccaaag acaaaaagtc agcatcaaaa
    32041 aatacttcta aaaaggtagc agctaaaaag aagaaaaaga aatctaagaa aaataaaaaa
    32101 taattt
//
LOCUS       DANBWH010000009        29209 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.009, whole genome shotgun sequence.
ACCESSION   DANBWH010000009 DANBWH010000000
VERSION     DANBWH010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 29209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29209
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.009"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            425..7600
                     /locus_tag="PPY92_000266"
     CDS             425..7600
                     /locus_tag="PPY92_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HDJ2624295.1"
                     /translation="MIMGNLRFQQEYFRIYKNNTESTTHRNAYWVKLAKNVEATKMMY
                     ALSTIVQQHASIRHFFDVTTDDNLTMILHEFLPFIEIKQVPSSSANYDLEAFFKQELS
                     TYHFNDSPLFKVKLFQFADAAYILLDFHVSIFDDSQIDIFLDDLCNAYRGNTVINNTR
                     QHAHINRNDDKDNQDASHIALDSNYFRLENNSDIHIDSYFPIKHPFEQALYQTYLIDD
                     MTSIDMASLAVSVYLANHIMSQQHDVTLGIHVPSHLPNDLHGNIVPLTLTIDAKDVCQ
                     RFTTDFNKCVLQNMSQLQCAKSSLSLETIFHCYHHMMSCCNDVIEDVHQIHDAHTSLA
                     DIEIFPHQHGFKIIYNSAAYDLLSIETLSDLVRNIYLQITEENGNKRTTVDELNLMTE
                     RDIQLYDDINLSLPEIDDAQTVVTLFEQQVEATPNHVAVQFDGVFITYQTLNARANDL
                     AHRLRNQYGVEPNDRVAVIAEKSIEMIIAMIGVLKAGGAYVPIDPNYPSDRQEYILKD
                     ATPKVVITYQALYENGKQNINHIDLNKIAWKNIDNLSKCNTLEDHAYVIYTSGTTGNP
                     KGTLIPHRGIVRLVHRNHYVPLNEETTILLSGTIAFDAATFEIYGALLNGGKLIVAKK
                     EQLLNPIAVEQLINENDVNTMWLTSSLFNQIASERIEVLVPLKYLLIGGEVLNAKWVD
                     LLNQKPKHPQIINGYGPTENTTFTTTYNIPNKVPNRIPIGKPILGTHVYIMQGERRCG
                     VGIPGELCTSGFGLAAGYLNQPELTADKFIKDSNINQLMYRSGDIVRLLPDGNIDYLY
                     RKDKQVKIRGFRIELSEVEHALERIQGINKAVVIVQNHDQDQYIVAYYEAMHTLSHNK
                     IKSQLRMTLPEYMIPVNFMHIEQIPITINGKLDKKALPIMDYVDTDAYVAPSTDTEHL
                     LCQIFADILHVNQVGIHDNFFELGGHSLKATLVVNRIEASTGKRLQIGDLLQKPTVFE
                     LAQAIAKVQEQNYEVIPEAIVKDDYVLSSAQKRMYLLWKSNHKDTVYNVPFLWRLSSG
                     LNVAQLQQAVQHLIARHEILRTQYIVVDDEVRQRIVADVVADFEEVNTHFTDEQEIMR
                     QFVAPFNLEKPSQIRVRYIRSPLHAYLFIDTHHIINDGMSNIQLMNDLNALYQHKLLL
                     PLKLQYKDYSEWMSHRDMTKHRQYWLSQFKDEVPILSLPTDYVRPNIKTTNGAMMSFT
                     MNQQMRQLLQKYVEKHQITDFMFFMSVVMTLLSRYARKDDVVVGSVMSARMHKGTEQM
                     LGMFANTLVYRGQPSPDKMWTQFLQEVKEMSLEAYEHQEYPFECLVNDLDQSHDASRN
                     PLFDVMLVLQNNETNHAHFGHSKLTHIQPKSVTAKFDLSFIIEEDRDDYTINIEYNTD
                     LYHSETVRHMGNQCMIMIDYILKHQDTLQICDIPNGTEELLNWVNTHVNDRMLNVPGN
                     KSIISYFNEVVSRQGNHVALVMNDLTMTYETLRNYVDAIAHMLLSNGVGNGQRVALFT
                     ERSFEMIAAMLATVKVGASYIPIDIDFPNKRQGAILEDAKVTAVMSYGVEIETTLPVI
                     QLENAKGFVESKENEQYDDLHGNQLENTAMLDNEMYAIYTSGTTGMPKGVAIRQRNLL
                     NLVHAWSTELQLGDNEVFLQHANIVFDASVMEIYCCLLNGHTLVIPDREERVNPEQLQ
                     QLINKHRVTVASIPLQMCSIMEDFYIEKLITGGATSTASFVKYIEKHCGTYFNAYGPS
                     ESTVITSYWSHHCGDLIPETIPIGKPLSNIQVYIMSDGLLCGIGMPGELCIAGDSLAI
                     GYINRPELMADKWQNNPFGKGKLYHSGDLARYTSDGQIEFLGRIDKQVKVNGYRIELD
                     EIENVILAIRGISDCVVTVSHFDTHDILNAYYVGEQQVEQDLKQYLNDQLPKYMIPKT
                     ITHIDCMPLTTNDKVDTTRLPNPSPIQQSNKVYSEPSNEIEQTFVDVFGEVLKQNDVG
                     VDDDFFELGGNSLEAMLVVSHLKRFGHHISMQTLYQYKTVRQIVNYMYQNQQSLVALP
                     DNLSELQKIVMSRYNLGILEDSLSHRPLGNTLLTGATGFLGAYLIEALQGYSHRIYCF
                     IRADNEEIAWYKLMTNLNDYFSEETVEMMLSNIEVIVGDFECMDDVVLPENMDTIIHA
                     GARTDHFGDDDEFEKVNVQGTVDVIRLAQQHHARLIYVSTISVGTYFDIDTEDVTFSE
                     ADVYKGQLLTSPYTRSKFYSELKVLEAVNNGLDGRIVRVGNLTSPYNGRWHMRNIKTN
                     RFSMVMNDLLQLDCIGVSMAEMPVDFSFVDTTARQIVALAQVNTPQIIYHVLSPNKMP
                     VKSLLECVKRKEIELVSDESFNEILQKQDMYETIGLTSVDREQQLAMIDTTLTLKIMN
                     HISEKWPTITNNWLYHWAQYIKTIFNK"
     gene            7613..8257
                     /locus_tag="PPY92_000267"
     CDS             7613..8257
                     /locus_tag="PPY92_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDJ2624296.1"
                     /translation="MTVFVMQLQSNLKSIEELISQSRWSYKKPRTVNYRYNQDKLMHR
                     LGDILVQYGIQHDTGLLPHEWHYHISPRGKADIVQHNRDGQPIYVSLSYSYPYIVCVV
                     DKEPVGIDIEKISQRLDWRTLVTCFSTNEAHQICSLNDFYQIWTQKESFTKLIGEGLI
                     KGLDIYDMTQSHFYQSREVKFKQFIFDQFMVQVCFLGEAPWGYKKVSVFQLLSS"
     gene            complement(8585..9064)
                     /locus_tag="PPY92_000268"
     CDS             complement(8585..9064)
                     /locus_tag="PPY92_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HDJ2624297.1"
                     /translation="MGIFIYAGIIGGLLSGIVKLGWEVMFPPRTPERNATNPPQELLQ
                     QLGFSSEFTHQTYTFSNMELPWVSFIVHFSFSIVIAIIYCILVKKYAYLAIGQGAVFG
                     IAIWVLFHLIIMPIMHTVPAVWDQPYQEHLSEFFGHIVWMMTIELVRQHFVYRYKLN"
     gene            complement(9334..10098)
                     /gene="argB"
                     /locus_tag="PPY92_000269"
     CDS             complement(9334..10098)
                     /gene="argB"
                     /locus_tag="PPY92_000269"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDJ2624298.1"
                     /translation="MKFIVIKIGGSTLSDMHPSIINNIKHLRSNNIYPIIVHGGGPFI
                     NEALSNQQIEPHFVNGLRVTDKATMTITKHTLIADVNTALVAQFNQHQCSAIGLCGLD
                     AQLFEITSFDQQYGYVGVPTALNKDALQYLCTKFVPIINSIGFNNHDGEFYNINADTL
                     AYFIASSLKAPIYVLSNIAGVLINDVVIPQLPLVDIHQYIEHGDIYGGMIPKVLDAKN
                     AIENGCPKVIIASGNKPNIIESIYNNDFVGTTILNS"
     gene            complement(10114..11355)
                     /gene="argJ"
                     /locus_tag="PPY92_000270"
     CDS             complement(10114..11355)
                     /gene="argJ"
                     /locus_tag="PPY92_000270"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HDJ2624299.1"
                     /translation="MKHQETTSQQYNFSIIKHGDISTPQGFTAGGMHIGLRANKKDFG
                     WIYSSSLASSAAVYTLNQFKAAPLIVTEDTLQKSKGKLQALVVNSANANSCTGQQGID
                     DARQTQTWVAQQLQIPSEHVAVASTGVIGEYLPMDKIKTGTEHIKDTNFATPGAFNEA
                     ILTTDTCTKHIAVSLKIDGKTVTIGGSAKGSGMIHPNMATMLAFITTDASIESNTLHQ
                     LLKSSTDHTFNMITVDGDTSTNDMVLVMANHQVEHQILSQDHPQWETFVDAFNFVCTF
                     LAKAIARDGEGATKLISVNVSGAKSISDARKIGKTIVSSNLVKSAIFGEDANFGRIIT
                     AIGYSGCEIDPKCTYVQLNQIPVVDKGMAVLFDEQAMSNTLTHEHVTIDVQLGLGNAA
                     ATAYGCDLSYDYVRINASYRT"
     gene            complement(11367..12398)
                     /locus_tag="PPY92_000271"
     CDS             complement(11367..12398)
                     /locus_tag="PPY92_000271"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDJ2624300.1"
                     /translation="MIKVGIVGGSGYGAIELIRLLQTHPHVTIAHIYSHSKVDEPLKL
                     TFPHLQHIMQHFEALTVDNNDCDVIFFATPAPVSKTCIPPLVEKGIHVIDLSGAFRIK
                     NREIYEAYYKETAAAQDDLNHAIYSISEWQSFDNNGTKLISNPGCFPTATLLALHPLI
                     SEKIVDLSSIIIDAKTGVSGAGRSLSQRVHFSEMNENLSAYAIGNHKHKPEIEQYLSI
                     IAGQDVSVIFTPHLVPMTRGILSTIYVKFSSEYTTESLHKLMTSYYANQPFVRIRDIG
                     TFPTTKEVLGSNYCDIGIYVDETTQTAILVSVIDNLVKGASGQAIQNLNILYDFEVTT
                     GLNQSPVYP"
     gene            complement(12437..13621)
                     /gene="rocD"
                     /locus_tag="PPY92_000272"
     CDS             complement(12437..13621)
                     /gene="rocD"
                     /locus_tag="PPY92_000272"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HDJ2624301.1"
                     /translation="MNSIIELTDYYSSNNYAPLKLVISKGKGVKVWDTDGKQYIDCIS
                     GFSVANQGHCHPTIVKAMTEQASKLSIISRVLYSDNLGKWEEKICHLAKKDKVLSLNS
                     GTEAVEAAIKIARKWGSEVKGITDGQVEIIAMNNNFHGRTLGSLSLSNHDAYKAGFHP
                     LLQGTTTVDFGDIEQLTQAISPNTAAIILEPIQGEGGVNIPPKGYIQAVRQLCDKHQI
                     LMIADEIQVGLGRTGKWFAMEWEQVVPDIYILGKALGGGLYPVSAVLANNDVMRVLTP
                     GTHGSTFGGNPLAIAISTAALDVLKDEQLVERSERLGSFLLKALLQLKHPSIKEIRGR
                     GLFIGIELNTDAAPFVDQLIQRGILCKDTHRTIIRLSPPLVIDKEEIHQIVAAFQDVF
                     KN"
     gene            complement(13880..15229)
                     /gene="brnQ"
                     /locus_tag="PPY92_000273"
     CDS             complement(13880..15229)
                     /gene="brnQ"
                     /locus_tag="PPY92_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDJ2624302.1"
                     /translation="MKKKLTFKENMFIGSMLFGLFFGAGNLIFPIHLGQAAGSNVFIA
                     NLGFLITAIGLPFLGIIAIGISKTSGLFEIASRVNKTYAYIFTIALYLVIGPFFALPR
                     LATTSFEIAFSPFLSPKQITLYLFIFSFVFFVIAWFFARKPSRILEYIGKFLNPVFLV
                     LLAIILLFAFIHPLGGISDAPISKQYQSHALFNGFLDGYNTLDALASLAFGIIIVATI
                     KKLGIENPTDIAKETIKSGTISIIMMGIIYTLLAIMGTLSIGHFKLSENGGIALAQIT
                     QYYLGNYGIVLLSLIVMVACLKTAIGLITAFSETFEHLFPKMNYLAIATVVSFISFLF
                     ANVGLTKIIMYSVPVLMFLYPLAIALIVLTLFSSKFHHSKIIYQCTIFFTMIAALVDG
                     LKASPEFISSTSFSQTLINFSQKYLPLSDIGMGWVVLSLIGFIIGFIIYKIKHRKIP"
     gene            complement(15508..16065)
                     /locus_tag="PPY92_000274"
     CDS             complement(15508..16065)
                     /locus_tag="PPY92_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HDJ2624303.1"
                     /translation="MINFNKTALVLIDLQEGILKMDYAPHTAENVVQNANKLIDVFRK
                     NNGFIAFVRVNFYDGKDVLQPNAMISLPPKEGDDYSRFHHLLDKRDDDFVIDKRHFSA
                     FVGTDLDLQLRRRGIDTIVLGGVATHIGVDTTARDAYQLNYNQFFVTDMMSAQNETLH
                     QFPIDNVFPLMGQTITTNDFLNILN"
     gene            complement(16132..17772)
                     /locus_tag="PPY92_000275"
     CDS             complement(16132..17772)
                     /locus_tag="PPY92_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-keto acid decarboxylase family protein"
                     /protein_id="HDJ2624304.1"
                     /translation="MKQRIGAYLIDAFHRAGVDKIFGVPGDFNLAFLDDIISNPNVDW
                     VGNTNELNASYAADGYARLNGLAALVTTFGVGELSAVNGIAGSYAERIPVIAITGAPT
                     RAVEQAGKYVHHSLGEGTFDDYRKMFAHITVAQGYITPENATTEIPRLINTAIAERRP
                     VHLHLPIDVAISEIEIPTPFEVTATKDTDASTYIELLATKLHQAKQPIIITGHEINSF
                     HLHQELEDFVNQTQIPVAQLSLGKGAFNEENPYYMGIYDGKIAEDKIRDYVDNSDLIL
                     NIGAKLTDSATAGFSYQFNIDDVVMLNHHNIKIDDVTNDEISLPSLLKQLSNISYTNS
                     ATFPAYHRPTSPDYTVGTEPLTQQTYFKMMQNFLKPNDVIIADQGTSFFGAYDLALYK
                     NNTFIGQPLWGSIGYTLPATLGSQLADKDRRNLLLIGDGSLQLTVQAISTMIRQHIKP
                     VLFVINNDGYTVERLIHGMYEPYNEIHMWDYKALPAVFGGKNVEIHDVESSKDLQDTF
                     NAINGHPDVMHFVEVKMSVEDAPKKLIDIAKAFSQQNK"
     gene            17965..18066
                     /locus_tag="PPY92_000276"
     CDS             17965..18066
                     /locus_tag="PPY92_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624305.1"
                     /translation="MMPQNTIAKKKQARHLMKVYAPVYSKHYLASNN"
     gene            complement(18045..20090)
                     /locus_tag="PPY92_000277"
     CDS             complement(18045..20090)
                     /locus_tag="PPY92_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HDJ2624306.1"
                     /translation="MRKKLFGQLQRIGKALMLPVAILPAAGLLLAIGTAIQGEALQHY
                     LPFIQNGGVQNVAKLMTAAGSIIFENLPMIFALGVAIGLAGGDGVAAIAAFVGYIIMN
                     KTMGDFLQVTPKNVTDPASGYASILGIPTLQTGVFGGIIIGALAAWCYNKFYNINLPS
                     YLGFFAGKRFVPIMMATTSFILAFPMALIWPTIQSGLNAFSTGLLDSNTGVAVFLFGF
                     IKRLLIPFGLHHIFHAPFWFEFGSWKNAAGEIIHGDQRIFIEQIREGAHLTAGKFMQG
                     EFPVMMFGLPAAALAIYHTAKPENKKVVAGLMGSAALTSFLTGITEPLEFSFLFVAPL
                     LFFIHAVLDGLSFLTLYLLDVHLGYTFSGGFIDYVLLGVLPNKTQWWLVIPVGLVYAV
                     IYYFVFRFLIVKLKYKTPGREDKQSQAVTASATELPYAVLEAMGGKANIKHLDACITR
                     LRVEVNDKSKVDVPGLKDLGASGVLEVGNNMQAIFGPKSDQIKHEMQQIMNGQVVENP
                     TTMEDDKDETVVVAEDKSATSELSHIVHAPLTGEVTPLSEVPDQVFSEKMMGDGIAIK
                     PSQGEVRAPFNGKIQMIFPTKHAIGLVSDSGLELLIHIGLDTVKLNGEGFTLHVEEGQ
                     EVKQGDLLINFDLDYIRNHAKSDITPIIVTQGNITNLDFKQGEHGNISFGDQLFEAK"
     gene            20675..21730
                     /locus_tag="PPY92_000278"
     CDS             20675..21730
                     /locus_tag="PPY92_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDJ2624307.1"
                     /translation="MTGFSVYLGQPLDEAYIKRMIKQGYQMIFTSVQIPEEDDETKYH
                     YFTKLLNLLKYEQVTYLIDANPSILTPSFYEHLRQYDAQFMIRIDHSTSIEAIEAIMA
                     QGLKCCLNASIISRELLTSLHQQLNDFTLLSFCHNYYPRPDTGLSVDLVNKKNELIYQ
                     FNPKAQIYGFIVGSGLRGPLHKGLPTIEATRHSHPVVAAKLLQETGVSEVLVGDSLIE
                     MRQAKQLIDFCKHRHFTLCIEEVFDTTVTYLFDMCHKVRPDNPENVIRSETSRQICPH
                     SIQPQFTTQRRIGSVTVDNLNNGRYQGEMQIVRQTLSAHDNVNVVAQIIKEDLPLLSC
                     IEPNDTFDFQKTRECKK"
     gene            21730..22626
                     /gene="murQ"
                     /locus_tag="PPY92_000279"
     CDS             21730..22626
                     /gene="murQ"
                     /locus_tag="PPY92_000279"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDJ2624308.1"
                     /translation="MENSTTEARNEATMHLDEMTVEEALITMNKEDQQVPLAVRKAIP
                     QLTKVIKKTIAQYKKGGRLIYIGAGTSGRLGVLDAAECVPTFNTDPHEIIGIIAGGQH
                     AMTMAVEGAEDHKKLAEEDLKNIDLTSKDVVIGIAASGKTPYVIGGLTFANTIGATTV
                     SISCNEHAVISEIAQYPVEVKVGPEVLTGSTRLKSGTAQKLILNMISTITMVGVGKVY
                     DNLMIDVKATNQKLIDRSVRIIQEICAITYDEAMALYQVSEHDVKVATVMGMCGISKE
                     EATRRLLNNGDIVKRAIRDRQP"
     gene            22638..24092
                     /locus_tag="PPY92_000280"
     CDS             22638..24092
                     /locus_tag="PPY92_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDJ2624309.1"
                     /translation="MTKEQQLAERIIAAVGGMDNIDSVMNCMTRVRIKVLDENKVDDQ
                     ELRHIDGVMGVIHDERIQVVVGPGTVNKVANHMAELSGVKLGDPIPHHHNDSEKMDYK
                     SYAADKAKANKEAHKAKQKNGKLNKVLKSIANIFIPLIPAFIGAGLIGGIAAVLSNLM
                     VAGYISGAWITQLITVFNVIKDGMLAYLAIFTGINAAKEFGATPGLGGVIGGTTLLTG
                     IAGKNILMNVFTGEPLQPGQGGIIGVIFAVWILSIVEKRLHKIVPNAIDIIVTPTIAL
                     LIVGLLTIFIFMPLAGFVSDSLVSVVNGIISIGGVFSGFIIGASFLPLVMLGLHHIFT
                     PIHIEMINQSGATYLLPIAAMAGAGQVGAALALWVRCKRNTTLRNTLKGALPVGFLGI
                     GEPLIYGVTLPLGRPFLTACIGGGIGGAVIGGIGHIGAKAIGPSGVSLLPLISDNMYL
                     GYIAGLLAAYAGGFVCTYLFGTTKAMRQTDLLGD"
     gene            24095..24970
                     /locus_tag="PPY92_000281"
     CDS             24095..24970
                     /locus_tag="PPY92_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDJ2624310.1"
                     /translation="MTNILYRIDKQLSDFTKTEKIIADYILKNPHKIIDMTVNDLADV
                     TNVSTASIVRFSRKMTHQGFQELKIAISRYLPEDIATNPHLELIENESVETLKNKMIA
                     RATNTMRFVATNIMDAQIDAICDVLKNARTIFLFGFGASSLTIGDLFQKLSRIGLNVR
                     LLHETHLLVSTFATHDDRDCMIFVTNQGSHSELQSIAQVATHYSIPIITISSTANNPV
                     AQIADYALIYGRTDENEMRMAATTSLFAQLFTVDILYYRFVALNYHAILDCITQSKMA
                     LDNYRKHLATIDFKH"
     gene            complement(25164..25445)
                     /locus_tag="PPY92_000282"
     CDS             complement(25164..25445)
                     /locus_tag="PPY92_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624311.1"
                     /translation="MVLITYQIILFFIISLSYYLTLNHYMAVTVGNFTSIFGMFAAIL
                     FMYYYLLYKSPEYNQRKRFKHFIHITNLIIIAFSTFVLVHLALKLFFSI"
     gene            25658..28447
                     /locus_tag="PPY92_000283"
     CDS             25658..28447
                     /locus_tag="PPY92_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDJ2624312.1"
                     /translation="MAYQSEYALENEMMNQLEQLGYERVTIRDNKQLLDNFRTILNER
                     HADKLEGNPLTDKEFQRLLTMIDGKSIFESARILRDKLPLRRDDESEIYLSFLDKKSW
                     CKNKFQVTNQVSVEDTYKARYDVTILINGLPLVQVELKRRGIDINEAFNQVKRYRKQN
                     YTGLFRYIQMFIISNGVETRYFSNNDSELLKSHMFYWSDKQNNRINTLQSFAESFMRP
                     CQLAKMISRYMIINETDRILMAMRPYQVYAVEALIQQATETGNNGYVWHTTGSGKTLT
                     SFKASQILSQQDDIKKVIFLVDRKDLDSQTEEEFNKFAKGAVDKTFNTSQLVRQLNDK
                     SLPLIVTTIQKMAKAIQGNAHLLEQYKTNKVVFIIDECHRSQFGDMHRLVKQHFKNAQ
                     YFGFTGTPRFPENSSQDGRTTADIFGRCLHTYLIRDAIHDGNVLGFSVDYINTFKNKA
                     LKAEDNSMVEAIDTEEVWLADKRVELVTRHIINNHDKYTRNRQYSSIFTVQSIHALIK
                     YYETFKRLNKKLEQPLTIAGIFTFKPNEDDRDGEVPYHSREKLEIMISDYNKKFETNF
                     STDTTNEYFNHISKNVKKGVKDSKIDILIVVNMFLTGFDSKVLNTLYVDKNLMYHDLI
                     QAYSRTNRVEKESKPFGKIVNYRDLKKETDDALRVFSQTNDTDTILMRSYEEYKKEFM
                     DAYRELKMIVPTPHMVDDIQDEEELKRFVEAYRLLAKIILRLKAFDEFEFTIDEIGMD
                     EQENEDYKSKYLAVYDQVKRATAEKNKVSILNDIDFEIEMMRNDTINVNYIMNILRQI
                     DLEDKAEQRRNQEQIRRILDHADDPTLRLKRDLIREFIDNVVPSLNKDDDIDQEYVNF
                     ESIKKEAEFKGFAGERSIDEQALKTISNDYQYSGVVNPHHLKKMIGDLPLKEKRKARK
                     AIESFVAETTEKYGV"
     gene            complement(28594..29193)
                     /locus_tag="PPY92_000284"
     CDS             complement(28594..29193)
                     /locus_tag="PPY92_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624313.1"
                     /translation="MSLSKLPLFNHFANIRIQPKLIIKLFLTLIIIVTYTFVSGLSVD
                     YKKLLSEAGLSETSLAQIKIFSQISIFFSRVFSVLFTFLIILVISRIMRSDANTKSIF
                     SATLSYILFTNAISLIILTIQISAGLSLIDYSITSLNIFAKGNRILGTFDLQFFVQAY
                     ILTVMLIGTSKLSIRASTIWGIIYLILIITFSLISISIQ"
BASE COUNT         9848 a         4586 c         5546 g         9229 t
ORIGIN      
        1 tgcatctttt tatattgaaa acctattaat ttggtcgtga ttaaaataga gataaattta
       61 ttaagaattt gttaaaattt aaggataaca attgttttat gcgagttgtt tgtttaatat
      121 aaacttgctt catggatatt ttgtaacaac aatattgaat ttgcaatgag atatgaacaa
      181 cagaaagtga ttaacgagtt gtagtgtgct atttatcgaa gatgtatgta atttacattg
      241 aaaaatatac caattgtaac gatgatacaa gatgtatttt agggtagaat atatcgctca
      301 tatgttgcga atggtgtttt atattaatca ttacataacg agttaattaa catttcgtcg
      361 gttatcatat catcatgcaa ttttagtaag taatgagtat tatgatatga aagaaggact
      421 ttttatgatt atgggtaatt tgagatttca acaggaatat tttcgtatat acaaaaataa
      481 tacagaatca acgacacacc gtaatgcgta ttgggttaaa ctcgctaaaa atgttgaagc
      541 tactaaaatg atgtatgcat tatcgacaat tgtgcaacaa catgcatcta taagacattt
      601 ttttgatgtt actaccgatg acaatttaac aatgatactt catgaatttc tgccttttat
      661 tgagataaaa caagttccat cttcttccgc aaactatgat ttagaagctt tttttaagca
      721 agaattaagt acttaccatt ttaatgattc acctttattc aaagttaaat tgtttcagtt
      781 cgctgatgct gcatatatac tattagattt tcatgtgtcc attttcgatg atagtcaaat
      841 tgatattttt cttgatgatt tatgcaatgc atatcgtggc aatactgtta ttaacaatac
      901 tcgacagcat gcacatataa atagaaatga tgataaagac aatcaagatg catcgcatat
      961 agcattagac tcaaactatt ttcggttaga gaataactct gacatccata ttgatagtta
     1021 ttttccaatt aagcatccat ttgaacaagc tttatatcaa acgtatttga ttgatgatat
     1081 gacatcaata gatatggcat cgttggctgt tagtgtgtat ttagctaatc atataatgag
     1141 tcaacaacat gatgtcacat taggtataca tgtaccatca catttaccaa atgatttaca
     1201 cggaaatatt gtgccgttaa cgttaacaat cgatgcaaaa gatgtatgtc aacgttttac
     1261 aacagatttt aataaatgtg tgttgcaaaa tatgtcgcaa ttacagtgcg cgaagtcttc
     1321 gctttcacta gagactattt ttcattgtta tcatcatatg atgtcttgtt gtaatgatgt
     1381 tattgaggat gtacatcaaa tacatgatgc acatacatct ttagcggata ttgaaatttt
     1441 tccacatcaa cacgggttca aaattatata taacagtgca gcatatgatt tgctctcaat
     1501 cgagacgctg agtgacttag ttcgaaatat ttatttgcaa attactgaag aaaatggaaa
     1561 taaacgaaca actgtagatg aacttaattt gatgacagaa cgtgatattc aattatatga
     1621 cgatatcaat ttaagtttgc ctgagataga tgatgcgcaa acagttgtta ccttatttga
     1681 gcaacaagtt gaagcaacgc cgaatcatgt cgctgtgcaa tttgacggag tgtttataac
     1741 atatcaaaca ttgaatgcac gcgcgaatga tttagcacac cgtttgagaa accagtatgg
     1801 tgttgaacct aatgatcgtg tcgctgtcat agctgaaaaa agtattgaga tgataatagc
     1861 gatgataggt gtgttgaaag ctggtggggc ttacgtgcca attgatccga actatccaag
     1921 tgatcgtcag gagtacattt taaaagatgc aacgcctaaa gttgtaataa cgtaccaagc
     1981 tttatatgaa aatggtaaac aaaatattaa tcacattgat ttgaataaga tagcgtggaa
     2041 aaatattgat aatctttcta aatgtaacac gttagaagat catgcttatg ttatttacac
     2101 gtcggggaca actggtaacc ctaaagggac actaattccg caccgaggta ttgttcgctt
     2161 ggtccatcga aatcattatg taccattaaa tgaagagacg acaattttgt tatcaggaac
     2221 tatagccttt gatgctgcaa catttgaaat atatggtgca ttgctcaatg gtggaaagct
     2281 gattgttgct aaaaaagaac aattattaaa tccaatagcg gtagaacaat taatcaatga
     2341 aaatgacgtt aatactatgt ggttaacctc ctcattattt aatcagattg ctagtgaacg
     2401 aatagaagta ttggtaccgt taaagtattt attaattggt ggagaagtat tgaatgctaa
     2461 gtgggtggat ttgcttaatc aaaaaccgaa gcatcctcaa attattaatg gttatggacc
     2521 aactgaaaat acaacattta caacgacgta taatatacct aacaaagttc caaatcgtat
     2581 tcctattggt aaaccgattc tgggtactca tgtttatatc atgcaaggcg agcgtcggtg
     2641 tggcgttggt attcctggag aattatgtac aagtggcttt gggttagctg caggttattt
     2701 aaatcagcca gaattgacag cagataaatt tatcaaagat tcaaatataa atcagctgat
     2761 gtatagaagt ggtgatatcg ttcgtttgtt acccgatggc aacatagatt atttatatcg
     2821 aaaggacaaa caagttaaga ttcgagggtt taggattgag ttgtcagagg ttgagcatgc
     2881 gctcgagcgt atacaaggta ttaataaagc agttgttatt gttcaaaatc atgatcaaga
     2941 tcagtatatc gttgcttatt atgaagcgat gcatacatta tcacataata agattaaatc
     3001 acaattacgt atgaccttac cggagtacat gataccagtt aatttcatgc atattgagca
     3061 aattcctatt actattaatg ggaaattaga taagaaggca ttgcctatca tggactatgt
     3121 cgatacggat gcctatgtag caccgagtac agataccgaa cacttgctat gccaaatttt
     3181 tgcagatatt ttacatgtga atcaagtagg tattcatgat aatttctttg aattaggtgg
     3241 ccattcatta aaagcaacgt tagtggtgaa tcggatagag gcatctactg ggaaacgatt
     3301 acaaattggt gatttattac aaaagccaac tgtatttgaa ctagcacaag cgattgctaa
     3361 ggttcaagaa caaaactatg aagtgattcc agaagctata gttaaagatg attatgtgct
     3421 gagctctgca caaaagcgta tgtatttatt atggaaatca aaccataaag atacggtgta
     3481 taacgtacct tttttatggc ggttatcatc aggacttaat gtagctcaat tgcaacaagc
     3541 agttcagcat ttgatagcgc gacatgagat tctacgaaca caatatattg ttgtagatga
     3601 tgaggttcga caacgtattg tggcagatgt tgtagctgac tttgaagaag ttaacacgca
     3661 ttttacggat gaacaagaaa ttatgcgcca atttgtggca ccttttaatt tggaaaagcc
     3721 aagtcaaatt agagtgagat acattagaag tcccttacat gcatacctgt ttatagatac
     3781 acatcatatc attaatgacg gtatgagtaa tatacaatta atgaatgatc ttaacgcact
     3841 ttatcaacat aaattattgt taccacttaa attgcaatat aaagactata gtgagtggat
     3901 gtcgcatcgt gatatgacga aacatagaca atattggtta tctcaattca aagatgaagt
     3961 acctatttta agcttaccga cagactatgt tagaccaaat attaaaacga caaatggagc
     4021 aatgatgtca tttacaatga atcaacaaat gagacagcta cttcaaaagt atgtagaaaa
     4081 gcatcaaatt actgatttta tgttctttat gagtgtggtc atgacgttgt taagcagata
     4141 tgctcgaaaa gatgatgttg ttgtcggtag tgtgatgagt gcgcgtatgc ataaaggcac
     4201 ggagcaaatg ctaggcatgt ttgctaatac gttggtatat agagggcaac cgtcacctga
     4261 taaaatgtgg acacagtttt tacaagaggt taaggaaatg agtttggagg catacgagca
     4321 tcaagaatac ccattcgaat gtttagtaaa tgacttagat caatcacatg atgcctcacg
     4381 gaatccatta tttgatgtca tgttagtact acaaaacaat gaaacgaatc atgctcattt
     4441 tgggcatagt aaattaacac acattcaacc caaatcagtg acggcgaaat ttgatttatc
     4501 tttcatcatt gaagaagatc gcgatgacta tacaatcaat atcgagtata ataccgattt
     4561 atatcactca gaaacagttc gtcacatggg taatcaatgt atgattatga ttgattatat
     4621 tttgaagcat caagatacac tacaaatttg tgatatacca aacggtacgg aggaacttct
     4681 aaattgggtc aatacgcatg ttaacgatcg aatgcttaat gtcccgggaa ataaatctat
     4741 cataagttac tttaatgaag ttgtctcacg acaaggtaat catgttgcgc tagtcatgaa
     4801 tgatttgaca atgacgtatg aaacattacg caactatgtg gatgccattg cgcacatgct
     4861 cctatcaaat ggtgtgggca atggtcaacg ggttgccttg tttacagaac gtagttttga
     4921 aatgattgcg gcgatgttgg cgacagttaa agtaggtgca tcttatatac ctatcgatat
     4981 tgattttccg aataaacgac aaggtgcaat tttggaggat gctaaagtaa ctgcagtcat
     5041 gtcttacggc gttgaaattg aaacgacatt accagtcatt caattggaaa atgctaaagg
     5101 ctttgttgaa tcaaaggaaa atgaacaata tgatgattta catggcaatc aacttgaaaa
     5161 cacagcgatg ttagataatg agatgtatgc tatttacaca tctggtacga ccgggatgcc
     5221 taaaggggtt gccatacgac aacgaaattt gttgaattta gtgcatgcat ggtcaactga
     5281 attgcaatta ggcgacaatg aagtattttt gcaacatgca aatattgttt ttgatgcatc
     5341 agttatggag atttattgtt gtttgttaaa tggtcatacg cttgtgattc cagatagaga
     5401 ggaacgtgtt aatccagaac agttacaaca actcattaat aagcatcgtg tgacggttgc
     5461 gtcgattccg ttacagatgt gtagtattat ggaagacttt tatattgaaa agttgattac
     5521 aggcggggca actagtacgg catcctttgt taaatatatt gagaagcatt gtggcacgta
     5581 tttcaatgcc tatggaccat ctgagtcaac agtcatcaca tcgtattggt cacatcattg
     5641 tggtgatttg atacctgaga cgattccaat tggcaaaccc ttatctaaca tccaagtgta
     5701 tattatgtca gatggtttgt tatgcggtat tggtatgcca ggcgagttgt gtattgcagg
     5761 tgatagttta gcgataggat atattaaccg tccagaatta atggctgata aatggcaaaa
     5821 taatccattt ggtaaaggaa agttgtatca tagtggtgat ttagcacgtt atacatctga
     5881 tggtcaaatt gaatttttag gaagaataga taaacaagtg aaagttaacg ggtaccgtat
     5941 tgaacttgat gaaattgaaa atgtaatatt agctattcgt ggtatatctg attgtgttgt
     6001 aacagtaagt cactttgata cgcatgatat attgaatgct tattatgtcg gagagcaaca
     6061 agtggagcag gatttgaagc aatatttaaa tgatcagctg cctaagtata tgattcctaa
     6121 gactataacg catatcgatt gtatgccatt aaccacgaat gacaaggtgg atactacgcg
     6181 tttgccaaat ccatcaccta tacaacagtc taataaagtg tatagcgaac cctctaatga
     6241 aattgagcag acatttgttg atgtatttgg agaggtgttg aaacaaaatg atgtcggtgt
     6301 tgacgatgat ttctttgaac ttggtggtaa ctcattagag gcgatgttag ttgtctcgca
     6361 tttaaaacga tttggccatc atatttcaat gcagacatta taccaatata aaaccgtgcg
     6421 acagattgtt aattatatgt accaaaatca acaatcatta gttgcattac cggataatct
     6481 ttcggaatta caaaagattg ttatgtctcg ttataacttg ggtattttag aggatagtct
     6541 aagtcatcga cctctaggaa atacactatt gactggcgcg acaggttttt taggtgctta
     6601 tctgattgaa gcactacaag gatacagtca tcgcatttat tgtttcatac gtgctgataa
     6661 tgaggaaata gcatggtata agttgatgac gaatttaaat gattattttt cagaagagac
     6721 ggttgaaatg atgttatcaa acattgaagt cattgttggt gatttcgagt gtatggatga
     6781 tgttgtttta cctgaaaaca tggatacgat tattcatgca ggtgctcgta cggatcactt
     6841 tggtgatgat gatgaatttg aaaaagtaaa tgttcaaggt actgttgatg tcatacgttt
     6901 ggcacaacaa catcatgcaa ggttaatata tgtgtctacg ataagtgtgg gaacttattt
     6961 tgatatagac acagaagatg tgacattttc agaagcggat gtctataaag ggcaactact
     7021 aacatcacca tatacacgga gcaaatttta tagtgaatta aaagtattag aagctgtaaa
     7081 taatggctta gatggtcgga ttgtacgtgt tggtaatttg acgagtcctt acaatggaag
     7141 atggcatatg agaaatataa agactaaccg tttttcaatg gtaatgaatg atttgttaca
     7201 actggattgt atcggggtta gcatggctga aatgcctgta gatttttctt ttgtggatac
     7261 gactgcaaga caaattgtcg cattagcaca ggtcaacaca ccacaaatca tttaccatgt
     7321 gctatcacct aataaaatgc cggtgaaatc tttgttagaa tgcgttaagc gcaaagaaat
     7381 tgaactcgtc agcgatgaat catttaatga aattttacag aaacaagaca tgtacgaaac
     7441 gattggatta actagtgttg accgtgaaca acaactagca atgatagata caacattaac
     7501 attaaaaata atgaatcaca tcagtgaaaa atggccaacg ataactaaca attggctgta
     7561 tcattgggca caatatatca aaacaatatt caataagtaa gtagggaaag ttatgacagt
     7621 atttgtaatg caattacaga gtaacttgaa aagtattgaa gaattaatat cacaaagtcg
     7681 ttggtcatat aaaaaaccgc gtacagtcaa ctatagatac aatcaagata aactcatgca
     7741 cagattggga gatattttag tgcaatatgg aattcaacat gacacaggtt tattaccaca
     7801 tgaatggcat tatcacattt cgccacgagg taaggcagat attgttcaac acaatcgtga
     7861 tggacagccc atctatgtga gcttatcata tagttatcct tatatcgtgt gtgttgtcga
     7921 taaagaacca gttggtatag atatcgaaaa gatatcacaa cgtttagact ggcgtacgtt
     7981 agtgacgtgt ttctctacaa acgaagcaca tcaaatatgt agtttaaatg atttttatca
     8041 aatatggaca caaaaagaaa gttttacaaa attgattggt gaaggtttaa tcaaaggatt
     8101 ggacatttat gatatgacac aatcacactt ttatcaatca cgtgaagtga agttcaaaca
     8161 atttattttt gatcagttta tggtacaggt atgtttctta ggagaggcac cctggggtta
     8221 taaaaaagtg tctgtatttc agttattgag tagttaatat gacgtctgac agtatcattg
     8281 cgcagtgatt ttaaactata ctgcctaaat gtttactgtt agatgtattt ttattggaaa
     8341 aatgaaaggt gaatacacga tggcatcatt caaaatcatg acgagattaa tgtaagtgat
     8401 aaggtgaaaa tatactgagc aatattgaaa ttgccaaaaa taatatcttg ttacgacgca
     8461 tttgagactt taatgctcac gtaagttggc tggaatcata tcatgtcagt taataaaata
     8521 acgtgagcat caatcatcat actcaaacga tgatttaaaa ctcacgttaa tgttagtcag
     8581 tgtattaatt taatttatag cgatagacaa aatgttgtcg cactaattca attgtcatca
     8641 tccagacgat atggccaaag aattctgaca gatgctcttg gtatggttga tcccacacag
     8701 caggtacagt atgcatgatt ggcataatga taaggtggaa taatacccaa atagcaatac
     8761 caaaaacagc accttgtcca attgctaagt aagcgtattt tttaactaat atgcagtaaa
     8821 taattgcaat gacgatagaa aaactaaagt ggacaataaa gcttacccaa ggcaattcca
     8881 tatttgaaaa tgtatatgtt tgatgcgtaa actcactact aaatcctaat tgttgcaata
     8941 actcttgagg tgggttcgtt gcattacgtt ctggtgtgcg aggtggaaac atgacctccc
     9001 aacctaattt tacaattcca gataacaagc caccgataat tccagcataa atgaatatac
     9061 ccatttgtcg tttcgtcaac ttaaggactc ctctctattt actatcctca ttatagtatt
     9121 ttgtgaaaat aatcacaata aaaagctttg caaaacttaa aatattttga taaacaaatt
     9181 tgtaaaaggt tattagtttt ttacatctca tattgtatcg tactgtattc aaaattgtta
     9241 acgagatgaa cggtaaataa atgttttagc gcaatggata atcgaactgg aatcatctcg
     9301 cgtgtcaaaa cttgttaggc cttaatttca tagttatgaa ttaaggattg ttgtgccaac
     9361 aaaatcatta ttgtaaatag attcaatgat atttggcttg tttcctgatg caatgataac
     9421 tttaggacag ccattttcaa tcgcattttt ggcatctagc actttgggaa tcatacctcc
     9481 ataaatatca ccatgttcaa tatattgatg aatatcgact aatggcaatt gaggtataac
     9541 aacatcattg atgagtacac ctgcaatatt acttaataca taaataggcg cttttaatga
     9601 tgatgcaata aaataggcaa gcgtgtcagc attaatattg taaaattctc catcatggtt
     9661 attgaaacca atcgaattga tgataggtac aaatttagta cataaatact gtaaagcatc
     9721 cttatttaaa gcggtcggaa caccgacata tccatattgt tgatcaaaag atgtaatttc
     9781 aaacagctgt gcatccaaac cacataagcc tattgcagaa cattggtgct ggttaaattg
     9841 agctactaat gcagtgttaa cgtctgcaat gagcgtgtgt ttagtaatgg tcatggttgc
     9901 tttatcagtc actcttaggc cattaacaaa gtgtggctcg atttgctggt ttgataatgc
     9961 ttcattaata aatgggccac cgccatgaac gataatgggg tagatgttgt ttgatcgtaa
    10021 atgcttaatg ttgttaataa ttgatggatg catgtcacta agtgtactgc caccaatttt
    10081 aatgacaata aatttcatct aaccaacacc accttatgtt cgatatgatg cgttaatacg
    10141 cacataatca taggataaat cacaaccgta tgcagtcgct gcagcgttac ctaaaccaag
    10201 ttgaacgtca attgtgacat gttcatgagt taatgtgttc gacatagctt gctcatcaaa
    10261 tagtacagcc atacctttat ccacgacagg tatttggttc agttgaacat atgtgcattt
    10321 aggatcaatt tcacatccgc tatagccaat agctgtaatg attcgaccaa aattggcatc
    10381 ttcgccaaaa atagctgatt ttactagatt tgaacttacg atagttttac cgatttttct
    10441 tgcatctgat attgatttag cgcctgacac attgacgctg attaactttg ttgcgccttc
    10501 gccatctctg gctatagctt tagctaaaaa tgtacagaca aaattgaatg catcaacaaa
    10561 tgtttcccat tgtggatggt cttgactaag tatttggtgt tcaacttggt gatttgccat
    10621 gactaatacc atgtcatttg tacttgtatc gccatcaaca gtaatcatat taaatgtatg
    10681 gtcagtcgaa gattttaata attgatgaag tgtattcgat tcaatcgatg catcggttgt
    10741 tataaaagca agcatggtag ccatatttgg atgaatcata cctgaacctt tggcgctacc
    10801 accaattgta acggttttac catcgatttt tagtgataca gcgatatgtt ttgtacaggt
    10861 atcagttgtt aaaattgcct cgttaaacgc acctggcgtt gcaaaattag tatccttaat
    10921 atgttcggtc ccagtcttaa ttttatccat aggcaaatat tcaccaatga ccccagttga
    10981 agcaacagca acatgctcag atggtatttg aagttgttga gcaacccatg tttgtgtttg
    11041 tcgtgcatca tctatgcctt gttgaccggt acaagaattt gcattagctg aattaacaac
    11101 aagtgcttgt aattttcctt tagacttttg taaagtgtct tcagtgacaa taagtggtgc
    11161 agctttaaac tgatttaaag tatatacggc agctgaactt gccaaagacg atgagtaaat
    11221 ccacccaaag tcttttttgt tagcgcgtaa accgatgtgc ataccaccag ccgtgaagcc
    11281 ttgaggtgta ctgatatcgc catgtttaat aattgaaaag ttatattgtt gtgatgtcgt
    11341 ttcttgatgt ttcattctaa caccccttat ggataaactg gtgattgatt taggccagtc
    11401 gttacttcaa aatcatataa tatatttaaa ttttgaatgg cttgaccact tgcgcctttg
    11461 acaaggttat caatcactga tactaaaatt gctgtttgtg ttgtttcatc tacatagata
    11521 ccgatatcgc agtagttact accgagtact tcttttgtgg ttggaaaagt cccaatatct
    11581 ctaattctga caaatggctg attagcataa taagaggtca ttaatttatg taatgattca
    11641 gtcgtatatt cagatgaaaa tttgacataa attgttgata aaatacctcg tgtcattggt
    11701 acgagatgtg gtgtaaatat gactgataca tcttgacccg caatgataga taaatattgc
    11761 tcgatttccg gtttgtgttt atggtttccg attgcataag cacttagatt ttcattcatt
    11821 tctgaaaaat gaacacgttg tgataatgaa cgaccagcac ctgacacgcc ggtcttagca
    11881 tcaataataa tagatgacaa atctactatt ttttcgctaa taagtggatg taatgctaat
    11941 aatgttgctg tagggaaaca gccaggatta gaaatgagct tcgttccatt gttatcaaac
    12001 gattgccatt ctgaaatgct gtaaatagca tgattcaaat catcttgtgc tgcagcagtt
    12061 tctttgtaat atgcttcata tatttcacga ttcttaattc taaatgcgcc agataaatcg
    12121 ataacatgaa tacctttttc tactaaggga gggatacatg ttttacttac gggtgctggt
    12181 gtcgcaaaga aaattacatc acagtcatta ttgtccactg taagtgcttc gaaatgttgc
    12241 ataatatgtt gtaaatgtgg aaatgttaat ttcaacggtt catctacttt tgaatgtgag
    12301 tagatgtgtg caatcgttac atgaggatgt gtttgtaaca atcgaattaa ttcaattgcg
    12361 ccataaccgc taccgccaac gatacctact ttaatcatca tgaatccctc ctatgtcata
    12421 tataaatgat taattgttaa tttttaaaaa cgtcttgaaa agctgcaaca atttgatgga
    12481 tttcctcttt atcaataact agaggtggag acaatcgaat gatagtacga tgcgtgtctt
    12541 tgcataagat tccacgttga atcagttgat ccacaaaagg tgcagcatct gtgttaagct
    12601 ctatgcctat aaataaacca cgacctctaa tttctttaat actaggatgt ttaagttgta
    12661 gcaacgcttt taataaaaat gaacctaagc gttctgatcg ttcaaccagt tgttcatctt
    12721 taagtacatc aagcgctgcc gtcgatattg caatggctaa agggttacca ccaaatgttg
    12781 aaccatgtgt acctggtgtt agaacacgca tgacatcatt atttgcaagt acagcagata
    12841 cagggtataa gccgccaccc aatgccttac ctaaaatata aatgtctgga acaacttgtt
    12901 cccattccat cgcaaaccat ttcccagttc taccaagacc aacttgaatt tcatctgcaa
    12961 tcattaatat ttgatgttta tcacatagtt gacgcacagc ttgaatatat cctttcggtg
    13021 gtatattaac gccaccttca ccttgaattg gttccaaaat aattgctgct gtattcggtg
    13081 aaatagcttg tgttaattgt tcaatgtctc caaaatctac agttgtagtg ccttgaagta
    13141 gggggtgaaa tcctgcttta tatgcgtcgt ggttagatag tgataatgag ccaagtgtac
    13201 gaccgtgaaa attgttattc atagcgatga tttcaacttg tccgtcagta atgcctttaa
    13261 cttcagagcc ccattttcta gcaattttaa tggctgcttc aacagcttca gtaccagagt
    13321 taagggagag tactttgtct ttcttagcaa gatgacaaat tttttcttcc catttcccga
    13381 gattgtcact ataaaggaca cgtgaaatga tagacaactt tgaagcttgt tctgtcatcg
    13441 ctttaacaat tgttggatga caatggcctt ggtttgcaac tgaaaaaccc gaaatgcaat
    13501 ctatatattg tttgccatca gtatcccaaa ctttgacacc tttaccttta gaaatgacaa
    13561 gcttaagtgg tgcataatta ttagagctat aataatcagt taattcaatg attgaattca
    13621 tcggtctccc tcctttatat ttatacaatt taaagaatta atattaatat attacagttg
    13681 ttcaatataa gtgtcaaatg taaatttaat aatataaaga taaggttgtg tttgtattaa
    13741 tgtgatttgt aaaatgaaag tactaatatt ttttgtggat aatcagtgat atgtatgtat
    13801 acgattgcag tttaatgtag tatgaataaa aaatgccaca agcatatgtt taacatatga
    13861 ctgtggcata gtattatgct tatggaattt tacgatgctt aattttataa ataatgaagc
    13921 cgataatgaa accaatcaaa ctgagaacaa cccagcccat accaatgtct gataatggta
    13981 aatatttttg gctgaaatta atcaaagttt gtgagaatga tgtgcttgaa atgaactctg
    14041 gactagcttt taatccatct actaatgcag caatcattgt aaagaaaatg gtacattgat
    14101 aaataatttt tgaatgatgg aatttgctac taaataatgt tagtacaatc aaggcaattg
    14161 ctaatggata taagaacatt aacactggga ctgagtacat aataatctta gttaagccaa
    14221 cattcgcgaa taagaacgaa ataaagctta caactgttgc aatcgctagg taattcattt
    14281 tagggaaaag gtgttcgaat gtttctgaaa atgccgtaat caaaccgatg gctgttttta
    14341 aacaagcaac cataacgata agtgacaaca ggacgatacc gtagttacct aagtagtatt
    14401 gcgtaatttg cgctaaggca ataccaccat tttcactaag tttgaaatga ccaatactta
    14461 atgtacccat gattgctagt agggtataaa taatccccat cataatgata ctgatagtac
    14521 cagacttaat tgtttcttta gcgatatcag ttggattttc gatacctaac tttttaatcg
    14581 ttgcaacaat gatgatacca aatgccaatg acgctagcgc atctaaggta ttgtatccat
    14641 ctaaaaagcc gttaaataag gcatgtgatt gatattgttt actaataggt gcatcagata
    14701 tgccacctaa tggatggata aaagcaaata ataaaataat tgctaataat actaagaata
    14761 ccggatttaa aaatttaccg atatattcta aaattcttga tggctttctc gcaaaaaacc
    14821 atgcaatcac aaagaagacg aagctaaaaa taaataaata taaagtgatt tgctttggtg
    14881 ataaaaatgg cgaaaatgca atttcaaatg atgtcgttgc cagtctaggt aaggcgaaaa
    14941 atggtccgat aactagatat aaggcaatcg tgaaaatgta agcatatgtt ttattaacac
    15001 gcgatgcaat ttcaaataaa ccagatgtct ttgaaatgcc aatagcaatg atacctagaa
    15061 atggtaagcc aattgctgta attaaaaatc ctaagttagc gataaaaacg ttagaaccag
    15121 cagcttgacc caagtgtatt gggaagataa gattgccggc accaaagaat aaaccaaata
    15181 acatagaacc tataaacatg ttttctttaa atgttagttt cttcttcata tatgtatttg
    15241 ttctcctttt tagaacttat aaaaatctac ttaattaaga taatatcaaa aattaaacct
    15301 aaagtgatac ataattttgt aataaatact aaattttttg aaaattccgg aataaatact
    15361 gttttttatg tagtaggggc gtaagttaag aacgatttaa tagatatagg ctaagtttat
    15421 agataggagg ttagacaacc cgacgtatta atactttgta aaataaagat acccccttca
    15481 acatgatcga agggggtaag tgtatgttta gttcaatata tttagaaagt cgtttgtagt
    15541 tattgtttgt cccattaatg ggaatacatt atctattgga aattgatgta gcgtttcgtt
    15601 ttgtgcactc atcatatctg taacaaaaaa ctgattgtag tttaattgat aggcatctcg
    15661 cgctgtcgta tctacgccaa tatgcgttgc gacaccacca agaacaatcg tatcaattcc
    15721 tcgacgtcgc aattgtaagt ccaaatctgt tcctacaaat gcactaaaat gtcgtttgtc
    15781 tatgacaaaa tcgtcatctc tcttgtctaa taaatgatgg aaacgactgt agtcgtcgcc
    15841 ttcttttggt ggtaatgaga tcattgcatt tggttgcaat acatctttac catcatagaa
    15901 attcacgcga acaaaagcga taaagccatt gttttttcta aaaacatcta ttaatttatt
    15961 agcgttttga acgacatttt cagctgtatg tggcgcataa tccatcttaa gtataccttc
    16021 ttgcaggtcg attaacacta aagcggtttt attaaaatta atcatcttat atcagagtcc
    16081 ttttcgttta ataaagtatt cgcttaaact tataccctgt atacgatgaa attatttatt
    16141 ttgttgtgaa aaagctttag cgatatcgat cagtttcttc ggtgcgtctt cgacagacat
    16201 tttgacttcg acaaaatgca tcacatcggg atgaccatta attgcattaa acgtgtcttg
    16261 taaatctttt gatgattcaa cgtcatgaat ttcaacattt ttaccaccaa atacagctgg
    16321 taaagcttta taatcccaca tgtgaatttc attataaggt tcatacatgc cgtgaataag
    16381 tcgttctacc gtatagccgt cattattaat cacaaataat accggtttaa tatgctgtct
    16441 aatcatagtt gaaatagctt gaacagttag ttgcaatgag ccatcaccaa ttaataataa
    16501 gttacgacga tctttgtctg ctaattgtga acctaatgtt gcaggtaatg tatagccgat
    16561 agaaccccat aacggttgcc ctataaaagt attgtttttg tataatgcta aatcataagc
    16621 accaaagaat gatgtacctt gatcagcaat aatgacatca tttggtttta agaaattttg
    16681 catcatttta aaataagttt gttgtgttaa tggttctgtg ccaacagtat aatcgggtga
    16741 tgttggacga tgatacgcag ggaacgttgc actgttcgta tatgaaatat tggataactg
    16801 ttttaacaac gatggtagag atatttcatc atttgtaaca tcatcaattt tgatattgtg
    16861 atgatttaac ataacgacat catcgatatt gaattggtat gaaaatcctg ctgttgctga
    16921 atctgttaat ttggctccaa tatttaaaat taaatcgctg ttgtccacat aatctcgtat
    16981 tttatcttcg gcaattttcc cgtcgtaaat acccatataa tatggatttt cctcattaaa
    17041 agcacctttt cctaatgaaa gttgtgctac tggtatctgt gtttgattta caaaatcttc
    17101 taattcttga tggaggtgaa aactgttaat ttcatgtcca gtaatgatga taggctgctt
    17161 cgcttgatgc agtttagttg ctaataactc tatatatgtt gatgcatccg tatctttagt
    17221 tgccgtcact tcaaatggtg tcggtatctc aatttcagag attgcgacat cgattggtaa
    17281 atgtaaatga actgggcgtc tttcggcgat tgctgtatta attaaacgtg gtatttcggt
    17341 tgttgcattt tcaggtgtga tataaccttg tgcaacggtt atatgtgcaa acatttttcg
    17401 gtagtcgtca aatgtacctt caccaagtga gtgatgtaca tatttaccgg cttgttcaac
    17461 agcacgtgtc ggcgcacctg taatcgcaat gacaggtatg cgttcagcat atgaacctgc
    17521 gataccgttg acggcactta attcgccaac accaaatgta gtaactaatg cagcgagtcc
    17581 attaagacgg gcataaccgt ccgctgcgta acttgcgttt aattcatttg tatttcctac
    17641 ccaatctaca ttgggattgc tgataatatc gtctagaaaa gcgagattaa aatcaccagg
    17701 aacaccaaaa attttatcga cgcctgctcg atgaaaagcg tcaattaagt aagctccaat
    17761 gcgttgtttc ataaaaaaac caccttttct ctatatttac acttaaaatg atagcctcga
    17821 atacagtgta aggtcaagga agttgacttt gttgtgaaaa cgctaacaat agtgaacaat
    17881 taatactttt taggaaatga gtatgaaggg gggcttaaat tgatgatctt aaaattcaaa
    17941 atttaagggg agagcgaact atcaatgatg ccgcaaaata caatagcaaa gaaaaaacag
    18001 gcgcgtcacc ttatgaaggt atacgcacct gtttatagta agcattattt agcttcaaat
    18061 aattgatcgc caaatgaaat gttgccatgt tcaccttgtt taaaatcaag gtttgtaatg
    18121 tttccttgtg tcacgataat aggcgtaata tcactctttg catgattgcg gatgtagtct
    18181 aaatcaaagt tgattaataa atcaccttgt ttaacttctt gaccttcctc aacatgtaaa
    18241 gtaaagcctt ctccgtttaa tttaacagtg tctaaaccga tgtggattaa tagttctaaa
    18301 ccactatctg atacaagacc aattgcatgt tttgttggga aaatcatttg tattttaccg
    18361 ttgaatggtg cacgaacttc accttgtgaa ggtttaatag cgataccgtc acccatcatt
    18421 ttttcgctga acacttgatc aggcacttct gataaaggtg ttacttcacc agttaatggt
    18481 gcatgaacga tatggctcaa ttcgcttgtt gcagatttat cttctgcaac aacaacagtt
    18541 tcgtctttat cgtcttccat agtagtaggg ttttctacta cttgaccatt cataatctgt
    18601 tgcatttcat gtttgatttg gtcagattta ggaccaaaaa ttgcttgcat attattgccg
    18661 acttctaata caccagatgc gcctaaatct ttcaaaccag gaacatcaac tttagatttg
    18721 tcgttaactt caacacgtag acgtgtgata caagcgtcta aatgtttaat gtttgctttg
    18781 ccacccatag cttctaatac tgcatatggt aattcagttg ctgaagcagt aacagcttgt
    18841 gattgcttat cttcacgacc tggtgttttg tattttaatt ttacaattaa gaatcggaat
    18901 acgaagtagt aaataactgc gtatacaaga cctacaggaa tgactaacca ccattgtgtc
    18961 ttattaggta gtacgccaag taaaacgtag tcgatgaaac cacctgagaa agtataacct
    19021 aagtgaacat ccaataagta caatgttaag aatgataaac catcaagtac tgcgtgaata
    19081 aagaataata atggtgctac gaataagaat gagaattcta aaggttctgt aataccagtt
    19141 aagaatgatg ttaaagcagc agaacccatt aaacctgcta ctactttctt attttcaggt
    19201 ttagctgtgt gataaattgc taaagctgct gcaggtaaac cgaacatcat aacagggaat
    19261 tcaccttgca tgaatttacc agctgtcaaa tgtgcgcctt cacgaatttg ttcgataaag
    19321 atacgttggt caccgtgaat aatttcacca gctgcatttt tccatgaacc aaactcgaac
    19381 cagaacggtg cgtggaaaat gtgatgtaga ccgaatggaa ttaataaacg cttgatgaaa
    19441 ccaaataaga atacggcaac accagtattt gaatctaata atcctgtact gaatgcattt
    19501 aatcctgatt gaatcgttgg ccaaattaat gccattggaa atgctaaaat aaatgatgtt
    19561 gtagccatca taataggtac gaaacgctta ccagcgaaga aacctaaata agatggtaag
    19621 ttaatgttat agaacttgtt ataacaccaa gctgccaggg ccccgataat aataccgccg
    19681 aacacacctg tttgtaatgt tggaatacct aaaatgctag cgtaaccact cgctggatca
    19741 gtaacatttt taggtgtaac ttgtaaaaag tcgcccattg ttttgttcat gattatgtaa
    19801 ccgacgaatg ctgcgatagc tgctacgcca tcaccgccag ctaatccgat tgcgacacct
    19861 aatgcgaaaa tcataggtaa gttttcaaaa ataatactac cggcagctgt cattaattta
    19921 gcgacatttt gaacgccacc attttgtata aacggcaagt agtgttgtaa tgcttcgcct
    19981 tgtatagctg taccgatagc taataacaga cccgctgctg gtaaaattgc aacaggtaac
    20041 attagcgctt taccaatacg ttgcaattga ccgaaaagtt tcttcctcac ttgtccaacc
    20101 tccaaagttg tatgtaatat ttaagattca ataaaaaaga cacgagcaaa tagactgtgt
    20161 tggtaaccat cacagcttaa tttaactcat gcctaatctt acttagtaac acgttggtgt
    20221 atgtaattaa tgtaaaagaa gcaattgact aagtagtata taaaactgat acgttagttt
    20281 atctagctta ccatcacatc ttattgaatc ttatttgttt gcgactccta ttttagcatg
    20341 gtgaagtatg cgttttcaat acaattatta aattcaattt caaatctctt aaatgttcac
    20401 agattattat taaaacataa tataagataa tcctgaataa aatgtataaa agttgttgta
    20461 ataatccagc agtagcagct attatcagca cttaaaagaa aggataatct ttgatggtgt
    20521 aataattaac ttgtgacaga aatatgtaag attattacaa tgaacaggct ttaactgatt
    20581 aatattgtaa tgtgttttct cttatagtaa aatgtaagcg attacacaac tcaactatat
    20641 tttcctaata attatattga taaggagggc tactttgaca ggcttttcag tgtatttagg
    20701 acaaccttta gatgaagcgt atattaagcg aatgattaaa caaggttacc aaatgatttt
    20761 tacatctgta caaataccag aagaagatga cgagacaaaa tatcattatt tcacaaaact
    20821 actcaattta ttaaaatatg aacaagtgac ttacctcata gatgctaatc catctatttt
    20881 aacaccatct ttttatgagc atcttcgaca atatgatgca caatttatga ttcgtatcga
    20941 tcatagtaca tcaattgagg caatcgaagc gataatggca cagggtttaa agtgctgttt
    21001 gaatgcaagt attatttccc gggaattgtt aacaagctta catcaacaat tgaatgattt
    21061 tacattactt tcattttgtc ataactatta tccaagacca gatacgggat tatctgttga
    21121 cttggtcaat aagaaaaatg aactcattta tcaatttaat ccaaaggcac aaatatatgg
    21181 ttttattgta gggagtggtt tgcgaggtcc tttgcataaa ggcttgccaa caattgaagc
    21241 aacgagacat agtcatcctg tcgttgcagc taaattatta caagaaactg gtgtatctga
    21301 agtgttagtt ggagactcat tgattgaaat gaggcaggca aaacaactta tagatttttg
    21361 caagcatagg catttcacgt tatgtattga agaagtgttt gatacgacag tgacttacct
    21421 tttcgatatg tgtcataaag tacgcccgga taatccggaa aatgtcattc gttcggaaac
    21481 gtcaagacaa atatgtccac attcgattca accacagttt acgacgcaac gacgcattgg
    21541 ttcagtaacc gttgataatt tgaataacgg acgttatcaa ggcgaaatgc aaattgtgag
    21601 acaaacgctt agtgcacatg acaatgtgaa tgttgttgca caaattatta aagaagactt
    21661 accactgtta agttgtatcg agccgaatga tacatttgat tttcaaaaaa ctagggagtg
    21721 taagaagtga tggaaaatag tacgaccgaa gcgcgtaatg aagcgacgat gcatcttgat
    21781 gaaatgactg tggaagaggc tttaattacg atgaataaag aagatcagca agtcccgtta
    21841 gcagttcgaa aggcaatacc acaattgaca aaagtaataa aaaaaacaat tgcacagtat
    21901 aaaaagggtg gacgattgat ctatatcggt gcaggtacaa gtggaagatt gggtgtctta
    21961 gatgcagcgg agtgtgtacc tacattcaat actgaccctc atgaaattat aggtattatt
    22021 gctggtggac aacatgctat gacgatggct gtagaaggtg cggaagatca caaaaaatta
    22081 gcggaagaag atttgaaaaa tatagattta acatcaaaag atgtcgttat aggaattgcc
    22141 gcgagtggca aaacgccata tgttataggc ggtttaacat ttgctaacac aatcggtgct
    22201 acaacagtat ctatttcatg caatgaacat gcagttataa gtgaaattgc gcagtatcca
    22261 gtagaagtta aagttggtcc agaagtatta actggttcaa cacgtttaaa gtctggtaca
    22321 gcacaaaagt taattttaaa tatgatttca accatcacaa tggttggtgt cggaaaagtt
    22381 tacgataacc tcatgattga tgttaaagca accaatcaaa aactgatcga ccgttcagtg
    22441 cgtattattc aagaaatatg tgctatcaca tatgatgaag caatggcgtt atatcaggta
    22501 tctgagcatg atgtgaaagt tgcgacagtt atgggtatgt gtggcatttc taaggaagaa
    22561 gcaacaagac ggttattaaa caatggtgac attgttaaac gagcaatcag agatagacaa
    22621 ccttaggagg gatttaaatg accaaagaac aacaacttgc agaacgaatt attgctgcag
    22681 taggtggtat ggataatata gatagtgtca tgaactgcat gacacgtgtg cgtattaaag
    22741 tattagatga aaataaagta gatgaccaag aactaaggca tattgatggt gtcatgggtg
    22801 ttatacacga tgaacgcatt caagttgtgg ttggacctgg tacagtcaat aaagtggcta
    22861 atcatatggc ggaattaagt ggtgttaaac taggtgaccc aataccacac catcacaatg
    22921 atagtgaaaa aatggactat aaatcatatg cagctgataa agcaaaggcg aataaagaag
    22981 cacataaagc aaaacaaaag aatggtaagt tgaataaagt attgaaatca attgccaata
    23041 tctttatacc gttgattcct gcatttattg gagctggatt aattggtggt attgcagcag
    23101 tactgagtaa cttaatggtg gcaggctata tttcaggtgc ttggattacg caacttataa
    23161 cagtattcaa tgtcattaaa gacggtatgt tagcatactt agctattttt actggtatta
    23221 atgcggctaa agaatttggt gcaacaccag gacttggtgg cgtgattggt ggtacaacgt
    23281 tattaacggg tattgctggt aaaaatattt taatgaatgt cttcactgga gaaccattgc
    23341 aacctggaca aggtggaatt attggcgtta tttttgccgt ttggatttta agtattgtcg
    23401 aaaagagatt gcataaaatt gtgccaaatg cgattgatat tattgtaacg ccgactattg
    23461 cattgttgat tgtaggacta ttaactatct ttatctttat gccattagca ggttttgttt
    23521 cagacagttt agtttcagta gttaacggaa ttattagtat tggtggcgta tttagtggat
    23581 ttatcattgg agcaagcttc ctaccgttag ttatgttagg gcttcatcat atttttacgc
    23641 caattcatat agaaatgatt aaccaatctg gtgctactta cttattgcca attgcagcga
    23701 tggctggtgc tggacaagta ggtgccgcat tagcactttg ggtaagatgt aaacgcaaca
    23761 caacattacg taatacttta aaaggtgcat tgccagttgg tttcctaggt atcggagaac
    23821 cattaatcta tggtgtgact ttgccattag gtcgaccttt cttaactgct tgtattggtg
    23881 gtggtattgg tggcgctgta ataggtggaa ttggacatat tggtgccaaa gcaataggcc
    23941 caagtggtgt gtcactctta ccattaatct cagataatat gtatttaggt tatattgcag
    24001 gattacttgc tgcatatgct ggtggattcg tttgtacata tttatttgga acgacaaagg
    24061 cgatgcgaca gacagatttg ttgggtgatt aatgatgaca aatattttat atcgcattga
    24121 taaacagttg agtgatttta cgaagacaga aaagataatc gctgattaca ttttaaagaa
    24181 tccacataaa atcattgata tgacggtgaa tgatttggca gatgttacga atgttagtac
    24241 agcatcaatt gttagattta gtcggaaaat gacacatcaa ggttttcaag agctaaagat
    24301 tgcgatatct cgatacttac ccgaagatat tgcaaccaat ccacatttag aattgattga
    24361 aaatgaatct gtagaaactt tgaaaaataa aatgattgct agagcaacga atacgatgcg
    24421 atttgtagct actaatatta tggatgcgca aattgatgca atttgtgatg tgttgaaaaa
    24481 tgccaggaca atatttttat ttggatttgg cgcatcgagt ttgactattg gtgatctttt
    24541 tcaaaagtta tctcgtattg gcttaaatgt caggttatta catgaaacgc atttacttgt
    24601 gtcaacattt gcgacgcatg atgatagaga ttgcatgatt tttgtgacga atcaaggtag
    24661 tcatagtgaa ttgcagtcaa ttgcacaggt ggccacacat tacagtattc ccatcataac
    24721 tatatctagt acagctaata atccagtggc tcaaattgca gactatgcat tgatttatgg
    24781 cagaactgat gaaaatgaaa tgcgtatggc ggctacaact tcactatttg cacagttatt
    24841 cacggtagat atattgtact atcgatttgt agcattaaat tatcatgcga ttctagattg
    24901 tataacccaa tcgaaaatgg cacttgataa ttaccggaag catcttgcga cgatagattt
    24961 taaacattag atattgtgag cgttctaata taaagttagt tgcaacgtaa acaattgaag
    25021 tttttgatgt gattcagttg tttgatgaaa aattgttgct acaagtttgt aaaactggca
    25081 tagaggctat tttgaaaatg ataaaggcgt gacaaattaa agcaaagttc aggtttgagc
    25141 tttgcttttt tcatagatgg aaattaaatg ctgaagaata attttaatgc taaatgaact
    25201 aatacgaagg tgctaaaagc aattattatc aaattagtga tatgaatgaa atgtttaaat
    25261 cgtttgcgtt gattgtattc gggactttta tagagtaggt agtagtacat aaagagtatg
    25321 gctgcgaaca tgccgaatat tgaagtgaag ttacctacag tgactgccat gtaatgattt
    25381 aaagttaaat agtaacttag actaataata aaaaataaaa tgatttgata ggtaattaac
    25441 accattcaag tccctccatt aatcgtagac aaaaaattta tcaatgattt gattatttgt
    25501 attcaaattt tagtatacga cttacctcaa agacaaatat taaaagataa gacaataaaa
    25561 ttagaataaa tttacgtttg aagataaaaa acaaacattt ttcattaaag tttatgatat
    25621 atttagagca atagaaagtg tatggaaggg gatatgaatg gcataccaaa gtgaatacgc
    25681 attagaaaat gaaatgatga atcaacttga acaattgggt tacgaaagag taacgatacg
    25741 tgataataag caattgcttg ataattttag aacgatttta aatgagcgtc atgcggacaa
    25801 attagaaggc aatcccttaa cagataaaga atttcaacgt ctgttaacga tgattgatgg
    25861 gaaaagtatt ttcgagagtg cccgtatttt acgtgataaa ttaccactta gacgtgatga
    25921 tgagtctgag atttatttgt cgtttttaga taagaaaagt tggtgtaaaa ataagtttca
    25981 agtgacgaat caagtatctg tcgaggatac atataaagca cgttatgatg taacgatatt
    26041 aatcaacgga ctaccccttg tccaagttga attgaaacgt cgaggtattg atattaatga
    26101 ggcgtttaac caagtaaaac gttaccgcaa acaaaattac acaggcttat tccgctacat
    26161 acaaatgttt atcattagta atggtgttga aacgcgatac ttttctaata atgatagcga
    26221 actattgaag agtcacatgt tttattggag tgataaacag aataaccgaa tcaatacatt
    26281 acaatcattt gctgagtcgt ttatgagacc ctgtcaatta gctaagatga tatcgcgcta
    26341 tatgattatt aatgaaacag atagaatact gatggcaatg cgtccgtatc aagtgtatgc
    26401 ggtagaagca cttattcaac aagcgactga gacagggaat aatggatatg tatggcatac
    26461 aactggaagt ggtaagacat tgacttcttt taaagcgagt cagattttat cacagcaaga
    26521 tgacattaag aaagttatct ttttggttga ccgtaaagac ttggatagtc aaacagaaga
    26581 ggaatttaat aaatttgcta agggtgctgt agacaaaact tttaatacct cgcaactggt
    26641 acgccaacta aatgataaaa gtttgccact tattgtaacg acgattcaaa aaatggctaa
    26701 agcgattcaa gggaatgccc atttattaga acagtataaa acgaataaag ttgtatttat
    26761 tattgatgag tgtcatcgca gtcaatttgg tgacatgcat cgtctagtta aacaacattt
    26821 caaaaatgcc caatactttg gattcactgg tacgccacgt tttccagaaa atagtagtca
    26881 agatggtaga acaactgcag atattttcgg tagatgctta catacgtatt taattagaga
    26941 tgccattcat gatggtaatg tacttggttt ctcagttgac tatattaata cttttaaaaa
    27001 taaagcttta aaagcagaag ataacagcat ggttgaagca attgatacgg aagaagtatg
    27061 gttagcggat aaacgtgtgg aattagtaac acgacatatc atcaataatc atgataaata
    27121 tacacgtaat cgtcaatatt caagtatatt tacagtccaa agtattcacg cgcttattaa
    27181 atattatgag acatttaagc gacttaacaa aaagttggaa caaccgttaa cgatagctgg
    27241 tatatttacg tttaaaccta atgaagatga tcgtgatggt gaagtgccat atcattcacg
    27301 tgaaaaatta gagataatga ttagtgatta taataaaaag ttcgagacga atttttcaac
    27361 agacacaact aatgagtatt ttaatcatat ttcaaaaaac gttaaaaagg gcgttaaaga
    27421 tagtaaaatt gatatcttaa tcgttgttaa tatgttctta actggttttg atagtaaagt
    27481 actgaacact ttatatgttg ataagaattt aatgtatcat gatttaattc aagcgtattc
    27541 acgtacaaat agggttgaaa aagaatcaaa gccatttggt aaaattgtaa actatcgtga
    27601 cttgaaaaaa gagacagacg atgcactgag agtattctca caaacaaatg atacggatac
    27661 aattttaatg cgcagttatg aagagtataa aaaagaattt atggacgctt atcgtgagct
    27721 taaaatgatt gtgccgacac cacacatggt tgatgacatt caagatgaag aagagctaaa
    27781 gcgctttgtt gaagcttatc gtttattagc taaaataata ttacgtttaa aagcatttga
    27841 cgagtttgag tttacaattg atgaaattgg aatggatgaa caagagaatg aagactataa
    27901 aagtaaatat ttagctgtgt acgatcaagt aaaaagagcg acggctgaga aaaataaagt
    27961 atccatttta aatgatattg atttcgaaat agaaatgatg cgtaatgata cgattaatgt
    28021 gaattatatt atgaatatat tgagacaaat tgatcttgaa gacaaagcgg aacaacgtcg
    28081 taaccaagaa caaattagac gcattttaga tcatgcagat gatccgacat tgaggttaaa
    28141 acgagatcta attagagaat tcatcgacaa tgttgtacct tctttaaata aggatgatga
    28201 tatcgatcaa gaatatgtta atttcgaaag tattaaaaaa gaagcggagt tcaaaggatt
    28261 tgctggagag agatctatcg atgaacaagc cctaaaaaca atttcaaatg actaccagta
    28321 tagtggtgtt gtaaacccac atcaccttaa aaaaatgatt ggtgatttgc cattgaaaga
    28381 aaagcgtaaa gcaagaaaag ccattgaatc tttcgtggca gaaacaactg aaaaatacgg
    28441 tgtgtaatga ttcagccccc tcgctagatt agtgtagggg gcattattat ttcattagaa
    28501 aaggctcatt tgttaaattc atcttatcaa gaatataacg aatgttcctt gttttaaact
    28561 tattaattaa tagagacatt ttatctgaaa atatcactga atgctaatac taatcaaaga
    28621 aaaagtgata attaaaataa gatatataat accccaaata gtggatgctc gtatagaaag
    28681 tttcgacgta ccaattagca tgactgtaag tatgtatgct tgtacaaaaa attgtaagtc
    28741 gaaagtaccc agaatgcgat ttcctttagc aaaaatattt aaactagtaa tagaatagtc
    28801 aattaatgat aaaccggcac ttatttggat ggtaagtata attaaactaa tagcatttgt
    28861 aaacagtatg tagcttaacg ttgcagagaa tatagatttt gtattagcat ctgagcgcat
    28921 aattctagag ataactagaa tgattaaaaa tgtaaataat acgctgaaaa ctcttgagaa
    28981 aaaaatacta atttgactaa aaatttttat ttgtgctaat gatgtctctg agagtcctgc
    29041 ttcactcaat aactttttat agtccacaga tagtccacta acaaatgtat atgtaacaat
    29101 tattatcaaa gtcagaaaca gcttaatgat taactttggt tgaatgcgga tatttgcaaa
    29161 gtgattaaac aaaggtagtt ttgataatga catatagacc tcctaatga
//
LOCUS       DANBWH010000010        28153 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.010, whole genome shotgun sequence.
ACCESSION   DANBWH010000010 DANBWH010000000
VERSION     DANBWH010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 28153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28153
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.010"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            1..258
                     /locus_tag="PPY92_000285"
                     /pseudo
     CDS             1..258
                     /locus_tag="PPY92_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144711.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            251..487
                     /locus_tag="PPY92_000286"
     CDS             251..487
                     /locus_tag="PPY92_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HDJ2624314.1"
                     /translation="MNNREKIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEILE
                     GMTNAIQHSVKEGIELDEAVGIMAGQVVYKYEEE"
     gene            492..1025
                     /locus_tag="PPY92_000287"
     CDS             492..1025
                     /locus_tag="PPY92_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP pyrophosphatase"
                     /protein_id="HDJ2624315.1"
                     /translation="MTNTLQVKLLSENARMPERNHKTDAGYDIFSAETVVLEPQEKAV
                     IKTDVAVSIPEGYVGLLTSRSGVSSKTHLVIETGKIDAGYHGNLGINIKNDNETLESE
                     DMSNFGRSPAGIDGKYARLPVTDKILCMNGSYVINKGDKLAQLVIVPIWTPELKRVEE
                     FESVSERGAKGFGSSGV"
     gene            1062..1268
                     /locus_tag="PPY92_000288"
     CDS             1062..1268
                     /locus_tag="PPY92_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HDJ2624316.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITRAKSNQRFTVVEAESKEEAKEKY
                     EAQVKRDAVIKVGQLFENIRECGK"
     gene            1265..1459
                     /locus_tag="PPY92_000289"
     CDS             1265..1459
                     /locus_tag="PPY92_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624317.1"
                     /translation="MTDVKIKTISGGVYFVKTAEPFEKYVERTVNFNGYIYASTIIKQ
                     PTYIKTDTIESITLIEERGK"
     gene            1456..1659
                     /locus_tag="PPY92_000290"
     CDS             1456..1659
                     /locus_tag="PPY92_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624318.1"
                     /translation="MNQLRILLHDGSSLVLHEDELFNEIVFVLDDFRNDDDYLTIEKD
                     YGRELVLNKGYIVGINVEEADDD"
     gene            1652..1825
                     /locus_tag="PPY92_000291"
     CDS             1652..1825
                     /locus_tag="PPY92_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator RinB"
                     /protein_id="HDJ2624319.1"
                     /translation="MTKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDFAKL
                     GDQCDLMRAEVSE"
     gene            1826..2227
                     /locus_tag="PPY92_000292"
     CDS             1826..2227
                     /locus_tag="PPY92_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624320.1"
                     /translation="MYSKESIVNMIGTHKMKCNVLADVIPEYDSNSIAQYGIQATLPK
                     PQGENSSKVEDVVVRLERANKRYAQMLKEVEFINQSQQRLGHVDFCFLELLKKGYNRD
                     AIIKKMPNSKLNRNNFLARRDELAEKIYLLQ"
     gene            2578..3072
                     /locus_tag="PPY92_000293"
     CDS             2578..3072
                     /locus_tag="PPY92_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDJ2624321.1"
                     /translation="MIKLTPKQEKFVLGLIEGKSQRKAYIDAGYSTKGKSESYIDMQA
                     STVAKNNKVLIRYEELRQEVAEQSKWTRQKAFEEYEWLKNTAKNDIEIEGVKKATADA
                     FLASLDGMNRMTLGNEVLTNKKIETEIKMLEKKIDQMDKSENNSQEAEVAKALIKLAG
                     VNDD"
     gene            3065..4273
                     /locus_tag="PPY92_000294"
     CDS             3065..4273
                     /locus_tag="PPY92_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HDJ2624322.1"
                     /translation="MINEMLNPKQQEVWNCFINDKPKVLIASGAKRAGKTYVFILLFL
                     MHIATYKDKGLNFIIGGATQASIRRNILDDMELILGRELTLDKSNAVKIFGNKVYVFD
                     GQNSDAWKKARGFTSAGAFLNEGTALHNMFIKEVFSRCSYKGARILIDTNPENPMHPV
                     KKDYIDKSGQRLSNGRLNIKAFQFTLFDNTFLDEEYIESIIASTPTGMFTDRDIYGKW
                     VSAEGVVYKDFKEKVHYIKEEEFKTKQIKRKYAGVDWGYEHYGSIMVVAEDFDGNKYV
                     IEEHAHRHKEIDDWVAIAKGVIKRHGDILFYCDTARPEHIERFRREKIKARYADKAVI
                     AGIEVISRLFKLNKIFIIKEKVSLFKEEIYNYVWKDNADEPVKLNDDTLDALRYAVYT
                     ANKPSGTGFN"
     gene            4227..5705
                     /locus_tag="PPY92_000295"
     CDS             4227..5705
                     /locus_tag="PPY92_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDJ2624323.1"
                     /translation="MQFIQLISQVAQALIKGGNILYPSQPTQTEIFDAIVRTNNKPET
                     LEEMIVRYIKQHLEKLPEISIGQEYYEQRPDIVKEPKPVDATGAVDPLKPDDRMITNF
                     HANLVDQKVSYIVGKPIAFKHTDDEVVKRIDEVLGNRFDDKLHSVLTGASNKGIEWLH
                     PYLDEEGEFKLFRVPAEQGIPIWTDKEHEELEAFIRMYKLENETKVEYWDKVTVNYYV
                     YENGSLIPDYSNNLENSKTHFSTGSWGKIPFIPFKNNDLEISDIFMYKTLIDAYNRRL
                     SDLSNTFKDSNELTYVLTNYDDQELPEFKRLLRYYGAIKVSDNGGVDTIQVEVPVENS
                     KKYLDELYQKIMLFGQAVDFSSDKFGSAPSGVALEFLYTNLNLKADKLARKAKVAIQE
                     LLWFVFEHFDIKGEHKDVDISFNYNKVANTELQVQTAQQSMGIVSHETVLENHPFVED
                     LQAELERIEQEQMEYNKQLPNLDDGGADGAQQQERSNNKESE"
     gene            5644..6624
                     /locus_tag="PPY92_000296"
     CDS             5644..6624
                     /locus_tag="PPY92_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001795666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HDJ2624324.1"
                     /translation="MTEVLTVPNNKKDLTIKNQNDIDEYIDSLISKAEKPIEQLFANR
                     LKEIKQIIADMFEKYQSDDVYVTWTEFNKYNRLNKELTRIGTMLTDDYRQVAKMVQKS
                     QEDAYIEKFLMSLYLYETASQTSMQFDVPSKEVITSAIEQPIEFIRLVPTLQKHRDEV
                     LKKIRLHITQGIMSGEGYSKIAKAIRDDIGMSKAQSLRVARTEAGRAMSQAGLDSALV
                     AQKNGLQMYKYWQATKDTRTRDTHRHLDGAKKKIDEPFKSSGCVGQAPKLFVGVNSAK
                     ENINCRCKLMYYIDEDDLPSTTRVRKDDGTTEVIPQMTYREWEKYKRKRK"
     gene            6722..7318
                     /locus_tag="PPY92_000297"
     CDS             6722..7318
                     /locus_tag="PPY92_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HDJ2624325.1"
                     /translation="MDLYTLLGQFKDGEIDKQKVIDAIDESKSGMVPRSRLNDKNTEI
                     EELKEEISKRDKQIVKLQDSVKDDSEIQKELEELKNQNSEWETKYKETQLNNAVKLAV
                     AKEANDANDILAFINKDELELVDDGTVKGLDEAIKTLKESKPYLFASSKPVGKTPQGG
                     GNPDSGVTKERFDNMSVAERNELYLNDRETFEKLVNQN"
     gene            7339..8163
                     /locus_tag="PPY92_000298"
     CDS             7339..8163
                     /locus_tag="PPY92_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HDJ2624326.1"
                     /translation="MPQGITKTSNQIIPEVLAPMMQAQLEKKLRFASFAEVDSTLQGQ
                     PGDTLTFPAFVYSGDAQVVAEGEKIPTDILETKKREAKIRKIAKGTSITDEALLSGYG
                     DPQGEQVRQHGLAHANKVDNDVLEALMGAKLTVNADITKLNGLQSAIDKFNDEDLEPM
                     VLFINPLDAGKLRGDASTNFTRATELGDDIIVKGAFGEALGAIIVRTNKLEAGTAILA
                     KKGAVKLILKRDFFLEVARDASTKTTALYSDKHYVAYLYDESKAVKITKGSGSLEM"
     gene            8180..8506
                     /locus_tag="PPY92_000299"
     CDS             8180..8506
                     /locus_tag="PPY92_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HDJ2624327.1"
                     /translation="MYKVIERFEDAQDNGHEYQVGDTYPRDGLEVSEERFTELSTTNN
                     RRNLIAIKLVEDKQVEQSEASADEQKSLSDMKVSELKELAKKREIKGYSDMKKDELIK
                     VLEGVK"
     gene            8506..8820
                     /locus_tag="PPY92_000300"
     CDS             8506..8820
                     /locus_tag="PPY92_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015978403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HDJ2624328.1"
                     /translation="MDAKDVKMINGLSLNDSSNDEQIDYLIEEYKSVAEDYCNQKFDD
                     KEVPSGVKKFIAECIKFGTTGNISARTMGTVSYTYVTDIPSSAYAYLLPYRKLSWGKR
                     YV"
     gene            8813..9148
                     /locus_tag="PPY92_000301"
     CDS             8813..9148
                     /locus_tag="PPY92_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDJ2624329.1"
                     /translation="MFNPFDEFPHTIEIGEVEVVGTYPKEYERFKSNETIKGFMDTPT
                     SSETLKFHQMSKDFDRNLYTPYHIPITNKTLFNYEGKTYEVVGEPVDQGGQHEINLTR
                     LRVRPIGKG"
     gene            9135..9548
                     /locus_tag="PPY92_000302"
     CDS             9135..9548
                     /locus_tag="PPY92_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002480803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDJ2624330.1"
                     /translation="MAKVKYGNWDLVKELENYERDMERWVKRGIAKTTAKIHNTIISL
                     MPVDTGYLRESVTMDFKDSGFTGVINIGSEYAIYVNYGTGIYATGAGGSRAKKIPWSY
                     KDANGKWHTTKGQHAQPFWEPAIDAGRAFFNKYFS"
     gene            9561..9998
                     /locus_tag="PPY92_000303"
     CDS             9561..9998
                     /locus_tag="PPY92_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HDJ2624331.1"
                     /translation="MWVSVERYLFNKVYNKLKSNLIIQKQLDGRVFDCVQKDAVYPYI
                     VVGETNVTNKETTTSMVEDVGITLHVYSQARNRDEASQIIQFLGFVLNNEIEIDYYSF
                     IKSRIDTQEVITDIDQYTKHGIIRLVFKYRHNTLQRSVTNGAG"
     gene            9985..10545
                     /locus_tag="PPY92_000304"
     CDS             9985..10545
                     /locus_tag="PPY92_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDJ2624332.1"
                     /translation="MAQDKYIVALQIADKDLAKKLTIEEATLLGSLAEGGHTISNDLA
                     EIIQGGKKDYSRNSVEEEIKLTLDVVPGDKGQLALKESVKQFKQLRVWIWETKKRDGK
                     HHGVFAYVVIEEHEWSFDDEDNKIEITAKVKFNSADGTINDLPKEWLNPSALAPVVEF
                     EDMNAYEDSYENRTKKTTAGSSDLSM"
     gene            10607..11101
                     /locus_tag="PPY92_000305"
     CDS             10607..11101
                     /locus_tag="PPY92_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624333.1"
                     /translation="MTETTFNPITSLTINNEEVKAKATFMFDKTAKKFATEQEDNKGR
                     KQTISGFTNVYNALLERDTVAIVDFWECATAYLGKSAPKREDIEAEIMEIIERENDTL
                     NLLQGALDVMNNSGFFKQKSRLFWTQMNQAPSLAKEDEKEGAKAGIEMMKNNYKEIMT
                     VAPY"
     gene            11122..11463
                     /locus_tag="PPY92_000306"
     CDS             11122..11463
                     /locus_tag="PPY92_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015978311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624334.1"
                     /translation="MTSRYIGYMSNDELMSMLPAEWNDWIIGARQALIDQRDIALYGA
                     QYNAVAQAGKSLKRFVRQNEREHYIIRGQEDEYERMKQRELAKNKRKREIQKQGTRKF
                     LNSLKTSHKGG"
     gene            11466..14435
                     /locus_tag="PPY92_000307"
     CDS             11466..14435
                     /locus_tag="PPY92_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDJ2624335.1"
                     /translation="MEKNFLARVTAIISDFKRNIRTAQRMAKTDIPDEIKTEVTANIR
                     DYQRELTRAKSMAQRWREHKVNIDADASKVKQVISFVKAELSNIRRKKVEIDGDASGL
                     KRNVATSKAMLAGWRKHTVKLDFDTTGMSKMQVALTAGKRALDQYQSTMDGIASNIRT
                     FGTIFAQQVKGLMIASIQALIPVIAGLVPAIMAVLNAVGVLGGGVIGLAGAFSVAGVG
                     AVGFGAMAITALKMVKDGTLAVTKEVQNFRDASDQLKTTWQGIVKENQASIFNAMSAG
                     IRGVTSAMSQLKPFLSEVSMLVEANAREFEDWVKHSETTKKAFEALNSIGGAIFGDLL
                     NAAGRFGDGLVNIFTQLMPLFKFVSQGLQNMSIAFQNWANSVAGQNAIKAFIDYTTTN
                     LPKIGQIFGNVFAGIGNLMIAFAQNSSNIFDWLVKLTSQFRAWSEQVGQSQGFKDFIS
                     YVQENGPTIMQLIGNIVKTLVAFGTAMAPIASKLLDFITNLAGFIAKLFETHPAIAQV
                     AGVMGILGGVFWALMAPIVAISSVLTNVFGLSLFSVTEKILDFVRTSSLVTGATEALI
                     GAFGSISAPILAVVAVIGAFIGVLVYLWKTNENFRNTITEAWNGVKTAVSGAIQGVVG
                     WLTQLWGKIQSTLQPIMPILQVLGQVFMQVLGVLVIGIITNVMNIIQGLWTLITIAFQ
                     AIGTVISVAVQIIVGLFTALIQLLTGDFSGAWETIKTTVTNVLDTIWQYMQSVWESII
                     GFLTGVMNRTLSMFGTSWSQIWSTITNFVSSIWNSVTSWFSRVASSVAEKMGQALNFI
                     ITKGSEWVSNIWNTVTSFASKVADGFKRVVSNVGDGMKNALDKIKSFFSDFLNAGAEL
                     IGKVAEGVANAAHKVVSAVGDAISSAWDSVTSFVSGHGGGSGLGKGLAVSQAKVMATS
                     FGKTFTSELGSTLTDGFNDSLTPSVDGHMTNDVQHSMKENNRPIVNVTVRNEGDLNMI
                     KSHIDDMDAKDGSFNLM"
     gene            14450..15385
                     /locus_tag="PPY92_000308"
     CDS             14450..15385
                     /locus_tag="PPY92_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HDJ2624336.1"
                     /translation="MIAHDVEIIKNGVKYRVSDNPHTYKHLRVLDYNVIGSGYKRNYS
                     PLDGVDGRFHNYAKEEYKKVELRLRYEVPKIAYASHLKSDIQTLFYGRFYLRELATPD
                     NTIKFENMFEPLEQEFELDYVDGRQLFVGLVSEVSFDTTKTSGEITLTFETTELPFFE
                     SIGYSTDLESDNDLEKWSVPDRIALNENDRSRQMTFYNTSSGDVYYNGDVALTQFNQF
                     NVVEIELAEDVKADDKDGFTFYTDKGNISVIKDVDLKAGDKIIFDNKHTYRGNLNIDL
                     YNKTLEQPVLYPGWNHFKANRLMKKIVFRHKLYYR"
     gene            15396..17282
                     /locus_tag="PPY92_000309"
     CDS             15396..17282
                     /locus_tag="PPY92_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDJ2624337.1"
                     /translation="MPVLLKSLQGVGHAIHVNTKLNEKLNEDSTLDIDMIENASTFDA
                     IGAITKMWTITNVKGEDDLNEYVIVMLDKSTIGNKIKLSIKARQKELDDLNNSRIYQE
                     YNESFTGVEFFNTVFKGTSYKYVLHTKVDASKFEGLGKGDTRLEIFKKGLERYHLEYE
                     YDAKTKTFHLYDELSKVANYYIKSGVNADNVKIQEDASKCYTYIRGYGDFDGQQTFTE
                     AGLQFEFTHPLAQLIGKREAPPLIDGRIKKEDVLKKSMELVIKKSVTASISLDFVAQP
                     EHFPEANPRIGDIVRVAEPTIGYNDLVRIVEITTHRDAYNNIIKQDVVLGDFTMRDRY
                     RKAIHEATNYVKNVKTTKSDPAKYLRELNAKVNASLSINNELVKQNEKINAKVDKMST
                     KTVTTANGTIMYDFTSQSSIRNIKSIGTIGDSVARGSHAKTNFTEMLGNKLKAKTTNL
                     ARGGATMATVPIGKEAVENSIYRQAEQIRGDLIILQGTDDDWLHGYWAGVPIGTDKTD
                     TKTFYGAFCSAIEVIRKNNPDSKILVMTATRQCPMRGTTIRRKDTDKNKLGLTLEDYV
                     NAQILACSELDVPVFDAYHTDYFKPYNPAFRKASMEDGLHPNEKGHEVIMYELIKDYY
                     SFYD"
     gene            17295..19193
                     /locus_tag="PPY92_000310"
     CDS             17295..19193
                     /locus_tag="PPY92_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624338.1"
                     /translation="MAYGLITSLHSMTGRKIVAQHEYNYRLLDEGMSKLEKMFIYHQK
                     EEIYAHSAKQIKYLNDSVEDYLTYLNGRFSNMILGHNGDGINEVKDARIDNTGYGHKT
                     LQDRLYHDYSTLDAFTKKVEKVVDEHYKEYRATEYRFEPKEQEPEFITDLSPYTNAVM
                     QSFWVDPRTKIIYMTQARPGNHYMLSRLKPNGQFIDRLLVKNGGHGTHNAYRYIGNEL
                     WIYSAVLDANNNNKFVRFKYRSGEMTYGNEMQDVMPNVFNDRYTSAIYNPIENLMVFR
                     REYKTSEQQAKNALNFVEVRSADDIDKGIDKVLYQMDIPMQYTSGTQPMQGIAYDAGI
                     LYWYTGDSNPANPNYLQGFDIKTKELLFKRRIDIGGVNNNFKGDFQEAEGLDMYYDLE
                     TGRKALLIGVTIGPGNNRHHSIYSIGQRGVNQFLKNIAPQVSMTDSGGRVKPLPVQNP
                     AYLSDITEVGHYYIYTQDTQNALDFPLPKAFRDAGWFFDVLPGHYNGALRQVLTRNST
                     GRNMLKFERVIDIFNKKNNGSWNFNPQSAGYWEHIPKSITKLSDLKIVGLDFYITTEE
                     SKRFSDFPKDYKGIAGWVLEVKSNTPGNTTQVLRRNNFASAHQFFVRNFGTGGNSGWS
                     IIEGKEVE"
     gene            19193..21016
                     /locus_tag="PPY92_000311"
     CDS             19193..21016
                     /locus_tag="PPY92_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HDJ2624339.1"
                     /translation="MVVDNFSKDDNLIELQTTSQYNPVIDTNISFYESDRGTGVLNFA
                     VTKNNRPLSISSEHVKTSIVLKTDDYNVDRGAYISDELTIVDAIDGRLQYVIPNEFLK
                     HSGKVHAQAFFTQNGSNNVVVERQFSFNIENDLVSGFDGITKLVYIKSIQDTIEAVGK
                     DFNQLKQNMADTQTLIAKVNDSATKGIQQIEIKQNEAIQAITATQTSATQAVTAEFSK
                     IVEKEQAIFARVNEVEKQINGADLVKGNTTTNWQKSKITDDYGKAIESSEQSIDSVLS
                     AINTSRIIHITSATDAPSFKDIGTLETPKEDGVDDGSEVSATTNTLGKSGLLVVYVVD
                     DSTARATWYPDDSNDEYTTYKIGGTWYQFYKKVDEELTKKFVKETSNNALNQAKQYVD
                     DKFGTTSWQQHKMTEANGQSIQVNLNNAQGDLGYLTAGNYYATRVPDLPGSVESYEGY
                     LSVFVKDETNKFFNFTPYNSKKIYTRSITNGRLEQQWTVPNEHKSTVLFDGGANGVGT
                     TINLTEPYTNYSILLVSGTYPGGVIEGFGLTTLPNAIQLSKANVVDSDGNGGGIYECL
                     LSKTSSTTLRIDNDVYFDLGKTSGSGANANKVTITKIMGWK"
     gene            21016..21393
                     /locus_tag="PPY92_000312"
     CDS             21016..21393
                     /locus_tag="PPY92_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2977 domain-containing protein"
                     /protein_id="HDJ2624340.1"
                     /translation="MKITVNDKNEVIGYVNTGGLRNSLDVDDNNVPIKFKEEFEPRKF
                     VFTNGEIKYNSNFEKEDVPNASNQQSASDLSDEELRGMVASMQMQMTQVNMLTMQLTQ
                     QNAMLTQQLTELKTNKTNTEGDV"
     gene            21397..21570
                     /locus_tag="PPY92_000313"
     CDS             21397..21570
                     /locus_tag="PPY92_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDJ2624341.1"
                     /translation="MKMIYPTFKDIKTFYVWGCYKNEQIKWYVDMGVIDKEEYALITG
                     EKYPEAKDEKSQV"
     gene            21611..21910
                     /locus_tag="PPY92_000314"
     CDS             21611..21910
                     /locus_tag="PPY92_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HDJ2624342.1"
                     /translation="MFGFTKRHEHEWRIRRLEENDKTMLSTLNEIKLGQKTQEQVNIK
                     LDKTLDAIQRERQIDEKNKKENDKNIRDMKMWILGLIGTIFSTIVIALLRTIFGI"
     gene            22047..23921
                     /locus_tag="PPY92_000315"
     CDS             22047..23921
                     /locus_tag="PPY92_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDJ2624343.1"
                     /translation="MGLPNPKTRKPTASEVVEWAKSNIGKRINIDNYRGSQCWDTPNF
                     IFKRYWGFVTWGNAKDMANYRYPKGFRFYRYSSGFVPEPGDIAVWHPGNGIGSDGHTA
                     IVVGPSNKSYFYSVDQNWVNSNSWTGSPGRLVRHPYVSVTGFVRPPYSKDTSKPSSTD
                     TSSASKANDSTITGEAKKPQFKEVKTVKYTAYSNVLDKEEHFIDHIVVMGDERSDIQG
                     LYIKESMHMRSVDELYTQRNKFISDYEIPHLYVDREATWLARPTNFDDPRHPNWLVIE
                     VCGGQTDSKRQFLLNQIQALIRGVWLLSGIDKNLSETTLKVDPNIWRSMKDLINYDLI
                     KQGIPDNAKYEQVKKKMLETYIKRDILTRENIKEVTTKTTIRISDKTSVDSASTRGPT
                     PSDEKPSIVTETSPFTFQQALDRQMSRGNPKKSHTWGWANATRAQTSSAMNVKRIWES
                     NTQCYQMLNLGKYQGISVSALNKILKGKGTLDGQGKAFAEACKKNNVNEIYLIAHAFL
                     ESGYGTSNFANGRYGAYNYFGIGAFDNDPDYAMTFAKNKGWTSPAKAIMGGASFVRKD
                     YINKGQNTLYRIRWNPKNPATHQYATAIEWCQHQASTIAKLYKQIGLKGIYFIRDKYK
                     "
     gene            23934..25172
                     /locus_tag="PPY92_000316"
     CDS             23934..25172
                     /locus_tag="PPY92_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HDJ2624344.1"
                     /translation="MYKIKDVETRIKNDGVDLGDIGCRFYTEDENTASIRIGINDKQG
                     RIDLKAHGLTPRLHLFMEDGSIFKNEPLIIDDVVKGFLTYKIPKKVIKHAGYVRCKLF
                     LEKEEEKIHVANFSFNIVDSGIESAVAKEIDVKLVDDAITRILKDNATDLLSKDFKEK
                     IDKDVISYIEKNESRFKGAKGDKGEPGQPGAKGDTGKKGEQGAPGKNGTVVSINPGTK
                     MWQIDGKDTNIKAEPELLDKINIANVEGLEDKLQEVEKIKDTTLNDSKTYTDSKIAEL
                     VDSAPESMNTLRELAEAIQNNSISESVLQQIGSKVSTEDFERFKQSLNSLYADKNHSH
                     TIKQIEGLENALSKKSDINHSHDERYLLSSNAFTKEEADKLYQPIGSSQPSLNIWTGS
                     ETEYNYLYQKDPNTLYLIKG"
     gene            25177..25572
                     /locus_tag="PPY92_000317"
     CDS             25177..25572
                     /locus_tag="PPY92_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624345.1"
                     /translation="MEGNFKNVKKLIYEGEEYTKVYAGNIQVWKKPSSFVIKPLPKNK
                     YPDSIEESTAKWTINGVEPNKSYQVTIENVRSGIMRVSQTNLGSSELGISGVNSGVAS
                     KNINFSNPSGTLYVTISDVYSGSPTLTIE"
     gene            25628..26065
                     /locus_tag="PPY92_000318"
     CDS             25628..26065
                     /locus_tag="PPY92_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDJ2624346.1"
                     /translation="MDINWKLRFKNKAVLTGLVGALLLFIKQITDLFGLDLSTQLNQA
                     SAIIGAILTLLTGIGVITDPTSKGVSDSSIAQTYQAPRDSKKEEQQVTWKSSQDSSLT
                     PELSAKAPKEYDTSQPFTDASNDVGFDVNEYHHGGGDNASKIN"
     gene            26046..27491
                     /locus_tag="PPY92_000319"
     CDS             26046..27491
                     /locus_tag="PPY92_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDJ2624347.1"
                     /translation="MQAKLTKKEFIEWLKTSEGKQFNVDLWYGFQCFDYANAGWKVLF
                     GLLLKGLGAKDIPFANNFDGLATVYQNTPDFLAKPGDMVVFGSNYGAGYGHVAWVIEA
                     TLDYIIVYEQNWLGGGWTDGIEQPGWGWEKVTRRQHAYDFPMWFIRPNFKSETAPRSV
                     QSPTQAPKKETAKPQPKAVELKIIKDVVKGYDLPKRGSNPKGIVIHNDAGSKGATAEA
                     YRNGLVNAPLSRLEAGIAHSYVSGNTVWQALDESQVGWHTANQIGNKYYYGIEVCQSM
                     GADNATFLKNEQATFQECARLLKKWGLPANRNTIRLHNEFTSTSCPHRSSVLHTGFDP
                     VTRGLLPEDKRLQLKDYFIKQIRAYMDGKIPVATVSNESSASSNTVKPVASAWKRNKY
                     GTYYMEESARFTNGNQPITVRKVGPFLSCPVGYQFQPGGYCDYTEVMLQDGHVWVGYT
                     WEGQRYYLPIRTWNGSAPPNQILGDLWGEIS"
BASE COUNT        10182 a         4393 c         6007 g         7571 t
ORIGIN      
        1 taacaagtta ttagataaac ttcacaaagc attaaaagat cgtgaagagt acaagaagca
       61 acgagatgag cttattgggg atatagcgaa gttacgagat tgtaacaaag aactggagaa
      121 gaaagcaagc gcatgggata ggtattgcaa gagcgttgaa aaagatttaa taaacgaatt
      181 cggtaacgat gatgaaagag ttaaattcgg aatggaatta aacaataaaa ttttatggag
      241 gatgacacaa atgaataatc gcgaaaaaat cgaacagtcc gttattagtg ctagtgcgta
      301 taacggcaat gacacagagg ggttgctaaa agagattgag gacgtgtata agaaagcgca
      361 agcgtttgat gaaatacttg agggaatgac aaatgctatt caacattcag ttaaagaagg
      421 tattgaactt gatgaagcag tagggattat ggcaggtcaa gttgtctata aatatgagga
      481 ggaataggaa aatgactaac acattacaag taaagctatt atcagaaaac gctagaatgc
      541 cagaacgaaa tcataagacg gatgcaggtt atgacatatt ctcagctgaa actgtcgtac
      601 ttgagccaca agaaaaggca gtgatcaaaa cagatgtagc tgtaagcata ccagagggct
      661 atgtcggact attaactagc cgtagtggtg taagtagtaa aacgcattta gtgattgaaa
      721 caggcaagat agacgcggga tatcacggca atttagggat taatatcaag aatgataatg
      781 aaacgttaga gagtgaggat atgagtaact ttggtcggag tcctgctggg atagatggaa
      841 agtatgcccg actacctgta acagataaaa ttttatgtat gaatggtagt tatgtcataa
      901 acaaaggcga caaactagct caattggtta ttgtgcctat atggacacct gaactaaagc
      961 gagtggagga attcgagagt gtttcagaac gtggagcaaa aggcttcgga agtagcggag
     1021 tgtaaagaca tcttagatcg agtcaaggag gttttgggga agtgacgcaa tacttagtca
     1081 caacattcaa agattcaaca ggacgcaagc atacacacat aactcgagct aaaagcaatc
     1141 aaaggtttac agttgttgag gcagagagta aagaagaagc gaaagagaag tacgaggcgc
     1201 aagttaaaag agatgcagtt attaaagtgg gtcagttgtt tgaaaatata agggagtgtg
     1261 ggaaatgacg gatgttaaaa ttaaaactat ttcaggtgga gtttattttg taaaaacagc
     1321 tgaacctttt gaaaaatatg ttgaaagaac ggtaaatttt aatggttata tttacgcaag
     1381 tactataatc aagcaaccaa cgtatattaa aacagatacg attgaatcaa tcacacttat
     1441 tgaggagcgt gggaaatgaa tcagctgaga attttattac atgacggtag tagtttggta
     1501 ttacatgaag atgaattatt taacgaaata gtatttgttt tggatgattt tagaaatgat
     1561 gatgactatt taacgataga aaaagattat ggcagagaac ttgtattgaa caaaggttat
     1621 atagttggga ttaatgttga ggaggcagac gatgactaaa caaatattaa gactattatt
     1681 cttactagca atgtatgagc taggtaagta tgtaactgag caagtatata ttatgatgac
     1741 ggctaatgat gatgtagagg cgccgagtga cttcgcaaag ttgggcgatc agtgtgattt
     1801 gatgagggcg gaggtgtcag aatagatgta tagcaaagag tcaattgtta atatgatagg
     1861 cacacataaa atgaagtgta atgtattagc tgatgtaata ccggaatatg atagcaattc
     1921 aatcgcacag tatggtatac aagcgacgtt accgaaacca caaggggaaa actcaagcaa
     1981 agttgaagat gttgttgtga ggcttgaaag agcaaataaa aggtatgcgc agatgttaaa
     2041 agaagttgag tttataaatc aatcacaaca gagattagga cacgttgact tttgcttctt
     2101 agagttgttg aagaaaggtt ataacagaga tgcaattatc aagaagatgc ctaactctaa
     2161 attgaacaga aacaacttct tagcgcgccg tgatgagtta gcagaaaaga tttatctact
     2221 acagtgacga aaatgacaaa aatgacagaa atgacgaaaa tgacactatt tttaaactgt
     2281 gaattaattt tatataattg atttgtaaga attatcttaa gacgtggggt aatagccaca
     2341 ttagatgttc tcatcgatgt gattgagaag tgacaaacat gtaaagttga tatgttacgc
     2401 tattaatcac ttactacctg cctatatggt gggtagttta attcttgcaa tttgagtcat
     2461 aactattttc ctcctttcac atttattgaa cgtagctcct gcacaagatg taggggcatt
     2521 tttgtattta aataactaga gtaattaacg taaaggcgtg tgatacagtg aaaacaattg
     2581 attaaattaa caccgaagca agaaaagttt gtattgggac tcatcgaggg caagagccaa
     2641 cgcaaagctt atattgacgc agggtattcg accaaaggta aaagtgaatc atatatagac
     2701 atgcaagcga gcacggtagc taaaaataat aaggttttaa taaggtacga agaacttcgt
     2761 caagaagtag ctgagcaatc aaaatggaca cgccaaaagg cttttgaaga gtatgagtgg
     2821 ctaaagaata cagcgaagaa cgatattgaa atagagggag tgaagaaagc gacagctgat
     2881 gcattcctcg ctagtttgga cggcatgaat agaatgacgt taggcaatga agttctgact
     2941 aataaaaaga ttgaaactga aatcaagatg cttgagaaaa aaatcgatca aatggataaa
     3001 tcagaaaata attcacaaga agcagaagtt gctaaagcac ttattaagtt agcgggtgtt
     3061 aatgatgatt aatgaaatgt taaacccgaa acaacaagaa gtctggaact gcttcataaa
     3121 cgataaaccc aaagtattaa tagcgagtgg tgcgaagagg gcaggtaaaa catatgtatt
     3181 tatcctgctt tttttaatgc acatagctac ttataaagac aaggggctta acttcattat
     3241 cggaggagcg acacaagcat ctatcagacg taacatacta gatgatatgg agttaatact
     3301 aggtagagag ttgacacttg ataaatctaa cgcagtcaaa atatttggta ataaagtgta
     3361 tgtattcgac ggacaaaact cggatgcatg gaaaaaagca cgtggtttta cttcagcagg
     3421 tgctttttta aacgagggga cagcattaca caatatgttt attaaagagg tattctcacg
     3481 ttgtagttac aaaggcgcga ggatattaat tgataccaac cccgaaaacc cgatgcatcc
     3541 agttaaaaaa gattacattg ataagagtgg tcaacggtta tcgaatggaa gactaaatat
     3601 caaagcattc caatttactt tgtttgacaa tacattttta gatgaagaat atattgaatc
     3661 gattatcgca agtacaccaa caggaatgtt cacagatcgt gatatttatg gtaagtgggt
     3721 ttctgctgaa ggtgttgtat ataaagattt caaagaaaaa gttcattaca tcaaagaaga
     3781 agaatttaaa actaaacaaa taaaaaggaa atatgcaggc gtcgactggg gatatgagca
     3841 ttatggttct attatggttg tagcggaaga ctttgacgga aacaagtacg ttattgaaga
     3901 acacgcacac agacataaag agatagatga ctgggtagct attgctaaag gagttataaa
     3961 aaggcatggc gatattcttt tttattgtga tacagctaga cctgaacata ttgaacgatt
     4021 tagaagagag aagataaaag caagatatgc tgacaaagcc gttattgctg gcattgaagt
     4081 tatttctagg ttattcaagt taaataaaat attcattatc aaagaaaaag ttagtttgtt
     4141 taaagaagaa atatacaact acgtttggaa agataatgca gacgaaccag ttaaattaaa
     4201 cgatgacaca ttagatgcgt taagatatgc agtttataca gctaataagc caagtggcac
     4261 aggctttaat taaaggaggt aatattttgt accctagcca accaacacaa acagaaatat
     4321 ttgatgctat tgtgaggact aacaataagc cagaaacact agaagaaatg attgtcagat
     4381 atataaaaca acatttggag aagttacctg aaatctcaat aggtcaagaa tattatgagc
     4441 aacgccctga tattgttaag gaacccaagc cggttgatgc tacaggagca gttgacccat
     4501 tgaaaccaga tgacagaatg attaccaact tccatgctaa cctagtagat caaaaagttt
     4561 cttatattgt agggaagcct atcgctttta aacatacaga tgatgaagta gttaaacgta
     4621 ttgatgaagt tttaggtaat agatttgatg ataagttaca cagtgtacta acaggagcta
     4681 gcaataaagg tattgaatgg ttgcaccctt accttgatga agagggagaa tttaagctat
     4741 ttagagtacc ggcggaacaa ggtattccta tatggactga taaagagcac gaagaattag
     4801 aggcgtttat caggatgtat aaattggaaa atgaaactaa agttgaatac tgggataaag
     4861 taactgttaa ttactacgtt tatgaaaacg gctcgcttat tccggattac tctaacaatt
     4921 tggagaattc aaaaacgcat tttagtacag ggtcatgggg taagattcca tttattccat
     4981 tcaaaaataa cgatttagaa atatcagata tatttatgta taaaacattg attgatgctt
     5041 ataacaggcg attatccgat ttatccaata cttttaaaga ttcaaacgaa ttaacgtatg
     5101 tattaacgaa ctatgatgac caagagttgc cagaattcaa acggttacta cgttattacg
     5161 gggcgataaa agtatcggat aacgggggtg tcgacacaat acaggtagaa gtaccagttg
     5221 aaaacagtaa aaagtattta gatgagttat atcaaaaaat aatgttgttt ggtcaagcgg
     5281 ttgactttag ttctgacaaa ttcggttcgg ctccaagtgg ggttgcgttg gagtttttat
     5341 atactaactt aaacttgaaa gcagataagt tagcgcgcaa agctaaagtt gctatacagg
     5401 agttgctttg gtttgtgttt gagcacttcg atatcaaagg agaacataaa gatgtcgata
     5461 ttagtttcaa ctacaacaaa gtagcgaaca cagaattaca agtacaaaca gctcaacaat
     5521 ctatgggaat tgtaagccat gaaacagtat tagaaaatca cccgtttgtc gaagatttgc
     5581 aagcagaact cgaacgaata gaacaagaac aaatggagta caacaagcaa ctgcctaatt
     5641 tagatgacgg aggtgctgac ggtgcccaac aacaagaaag atctaacaat aaagaatcag
     5701 aatgatattg atgagtatat cgacagtcta atctctaaag ctgagaagcc tatagaacaa
     5761 ctatttgcta atcgacttaa agagataaaa caaatcatcg cagatatgtt tgagaagtat
     5821 caaagtgatg atgtgtatgt tacatggact gaatttaata aatataacag gctcaataag
     5881 gagttaactc gtataggtac aatgttgact gatgactata ggcaagtagc taagatggtt
     5941 cagaagtcgc aggaagacgc ttatatagaa aagttcctta tgagccttta tttatacgag
     6001 acggcaagtc aaacatctat gcaatttgat gttcctagca aagaagttat cacatcggct
     6061 attgaacaac ctattgagtt cattcgatta gtaccgacac tacagaagca tcgtgatgaa
     6121 gtactgaaaa agatacgctt acatatcaca caaggcatta tgagcggaga gggctactct
     6181 aaaatagcga aagcaatccg tgatgatatt ggtatgtcta aagctcaatc gttgcgtgta
     6241 gctcgtacag aagcgggcag agcgatgtct caagctggac tcgatagtgc attggtagct
     6301 caaaagaatg gcttacagat gtataagtat tggcaagcta ctaaagatac acgtacaaga
     6361 gacacacaca gacatctaga cggtgctaag aagaaaatag acgaaccgtt caagtcgagc
     6421 ggttgcgttg gacaggcgcc taagttgttt gttggtgtga atagtgcaaa agaaaacatc
     6481 aactgtcgtt gtaagcttat gtattacatt gatgaagatg atttgcctag tacaacgaga
     6541 gtgcgtaaag atgatggcac aaccgaagta ataccacaaa tgacttatcg tgagtgggag
     6601 aaatataaac gtaaaagaaa gtagtttact actcgacctt agcatgtcgt taaactgctt
     6661 ctttttatac caaaattctt cgtggcgttg cacgtaaaac tcgtaaaaag gagtagttta
     6721 aatggattta tacacattgt taggacaatt taaagacgga gaaatcgata agcagaaagt
     6781 aattgatgcg attgacgaat caaaatcggg aatggtacca cgttcaagat tgaacgacaa
     6841 gaataccgaa attgaagagt taaaagaaga gatttctaaa cgtgataaac aaattgtcaa
     6901 attgcaagac tctgttaaag atgatagcga gattcaaaaa gaactcgaag aattaaagaa
     6961 tcaaaattca gagtgggaga caaagtataa agaaacacaa cttaataacg cagttaagtt
     7021 agcggttgct aaagaagcaa atgacgctaa cgacattcta gcattcatca ataaagatga
     7081 actggaatta gtagacgacg gcactgtaaa aggtttagat gaagcgatta aaacgcttaa
     7141 agagtctaaa ccttatttat ttgcgtcgtc taagcctgta ggtaaaacac cacaaggcgg
     7201 aggcaatccg gactcaggtg taacgaaaga aaggtttgac aacatgagtg tcgctgaacg
     7261 taacgaattg tatttgaacg atcgtgagac attcgaaaaa ttagttaatc aaaattaaac
     7321 aaagagagag gtataagtat gccacaagga attactaaaa caagtaatca aatcattcca
     7381 gaagtattag cgcctatgat gcaagcgcaa ctcgaaaaga aattgcgttt cgcttcattt
     7441 gcagaagtag atagcacatt acaaggacaa ccgggagaca ctttgacatt cccagcattc
     7501 gtttatagcg gagatgcaca agtagttgca gagggagaaa aaatcccaac tgatatctta
     7561 gaaactaaaa aacgtgaggc taaaatccgt aaaattgcta aaggtacatc tatcacagat
     7621 gaggctttat taagtggtta tggagaccca caaggcgaac aagtacgtca acatggtttg
     7681 gcacacgcta acaaagttga taatgacgta ttagaggctt taatgggagc taaacttact
     7741 gttaatgcgg acatcactaa attaaacggc ttacaatcag caatcgacaa atttaacgat
     7801 gaagacttag aaccaatggt tttatttatc aatccacttg atgctggtaa attacgtgga
     7861 gatgcatcaa ctaactttac gcgtgcaacc gaattaggcg atgacatcat cgttaaaggt
     7921 gcgtttggcg aagctctagg cgctatcatt gtacgtacta acaagttaga agccggcaca
     7981 gctattctag ctaaaaaagg tgcagttaaa ttaatcttga aacgtgattt cttcttagaa
     8041 gtagcgcgtg acgcatcaac aaaaacaact gcattataca gtgataagca ctatgtagct
     8101 tatttatatg atgaatctaa agcagtgaaa atcactaaag gttctggaag cttagaaatg
     8161 taatgggagg tagtgacgta tgtataaagt aatcgaacgt tttgaagatg cacaagacaa
     8221 cggacatgaa tatcaagtgg gagacactta cccacgtgat ggattagaag tgtcagaaga
     8281 acgattcact gaattatcta caacaaacaa ccgccgtaat ttaatcgcta ttaaacttgt
     8341 tgaagacaag caagtagaac agtctgaggc gagcgctgac gagcaaaaaa gtttatctga
     8401 tatgaaagta tcagaattaa aagaacttgc taaaaagcgt gaaattaaag ggtatagtga
     8461 tatgaaaaaa gacgagctta tcaaagtttt agagggtgtt aagtaatgga cgcaaaagac
     8521 gtcaaaatga ttaatggact ttcactcaat gattcgtcta acgatgagca aatcgattat
     8581 cttattgaag aatataaaag tgttgcagaa gattattgta atcagaagtt tgatgacaaa
     8641 gaagtgccgt cgggtgttaa gaagtttatt gctgaatgta tcaagtttgg tacaaccggc
     8701 aatatctcag cacgcacgat gggcactgtg agttatacct atgtaactga catacctagt
     8761 agtgcttatg catatttatt accttatcgt aaattaagct ggggcaagcg atatgtttaa
     8821 tccgtttgat gagtttccgc acacaattga aattggagag gttgaagtcg taggaacata
     8881 tcccaaagaa tacgagcgtt ttaaaagtaa cgaaacaatt aaagggttta tggacacacc
     8941 tacatcaagt gagacactca aatttcatca gatgagcaaa gactttgacc gaaacctata
     9001 tacgccgtac cacataccaa taacaaacaa aactttattt aattacgagg gtaaaacgta
     9061 cgaagttgta ggcgaaccgg tcgaccaagg cggacaacat gaaatcaatt taactagatt
     9121 gagggtgcga cctattggca aaggttaagt atggtaattg ggacttagta aaagagttgg
     9181 aaaattacga gcgagacatg gagcgatggg tcaaacgagg tatagcaaag acaactgcta
     9241 agattcacaa tacaatcatt tcattaatgc cagttgatac cggatatctt agagaaagtg
     9301 taacaatgga ctttaaagac agcggtttta ctggtgttat taatattggt agtgaatacg
     9361 caatatatgt caattatggt actggtatat atgcaacagg tgctggaggt agtagagcca
     9421 aaaagatacc gtggtcatac aaagatgcaa acggtaagtg gcacactact aaaggacaac
     9481 atgctcaacc tttttgggag ccggcaatag acgctggacg agcattcttt aataagtatt
     9541 tttcatgagg tggttaagat atgtgggtat cagttgaacg gtacttattt aacaaagtat
     9601 ataacaaatt aaaaagtaac cttattatcc aaaaacaatt ggacggtagg gtttttgatt
     9661 gcgttcagaa agacgctgtt tacccatata tcgttgtggg tgaaacaaac gtcactaaca
     9721 aagaaacgac cacgagcatg gtcgaagatg tcggcatcac gttgcatgtt tatagtcaag
     9781 cacgtaatag agatgaggca tcacaaataa ttcaattttt aggcttcgtt ttaaataacg
     9841 aaatagaaat tgattattat tcattcatta aaagtcgaat tgatacacaa gaagtgatta
     9901 ctgacataga tcagtacact aaacacggta tcattcggct tgtttttaaa tacagacata
     9961 acacattaca aaggagtgta acgaatggcg caggataaat atattgtcgc tctccaaatc
    10021 gctgataagg atttagctaa gaagctaact atcgaagaag caacgctttt aggtagttta
    10081 gcagagggtg gacatactat cagtaatgac cttgctgaaa tcattcaagg cggtaaaaaa
    10141 gattatagcc gtaactctgt cgaagaagaa atcaagttga cgcttgatgt cgttccggga
    10201 gataaaggtc aattagcatt aaaagaatca gttaaacaat tcaaacagtt acgtgtttgg
    10261 atttgggaaa cgaaaaaacg cgatggcaaa catcacggtg tattcgcata tgtagttatc
    10321 gaagagcacg aatggtcatt tgatgatgaa gataacaaaa tcgaaatcac agcgaaagtt
    10381 aagttcaata gtgcagacgg tacaatcaat gatttaccaa aagaatggct taaccctagc
    10441 gcattggctc cagttgttga attcgaagac atgaacgctt atgaagatag ttatgaaaac
    10501 cgaactaaaa aaacaactgc tggcagtagc gatttaagta tgtaattaac gagggcatta
    10561 gccctctatt tttttgtaca aaataacgat aaacgaggta tttaatatga ctgaaacaac
    10621 ttttaatcca attacatcat taacaattaa caatgaagaa gtgaaagcaa aagcaacatt
    10681 tatgttcgat aaaaccgcta aaaagtttgc aactgaacaa gaagataata aaggcagaaa
    10741 acaaacgatt tcaggattta ctaatgttta caatgcttta ttagaacgtg acacagtggc
    10801 aattgtagac ttttgggaat gcgcaacagc ttatctaggt aaaagtgcac ctaaaagaga
    10861 agacattgaa gcggaaatca tggaaatcat cgaaagagaa aacgacacgt taaatctatt
    10921 acagggtgcg ttggacgtaa tgaataatag cggttttttc aagcagaaat cacgtctatt
    10981 ctggacacag atgaaccaag cgccatcgtt agccaaagaa gacgagaaag agggcgcgaa
    11041 agctggtatc gagatgatga agaacaacta caaagaaatc atgaccgtag caccttatta
    11101 gactattcgg aaataaggca gatgacaagt cgttacatag gttatatgag taatgacgag
    11161 ctaatgagca tgctacctgc cgaatggaat gactggatta ttggcgctag acaagcattg
    11221 attgaccaaa gagacattgc gttgtacggc gctcaatata atgcggttgc tcaagctggt
    11281 aaatcactaa aacgttttgt taggcagaac gaaagagaac attacattat tcgtggtcaa
    11341 gaagatgaat acgaaagaat gaaacagcgt gagctagcta aaaacaaacg taaaagagaa
    11401 atacaaaaac aagggactcg caagttcctt aacagcttaa aaacaagtca taaaggaggt
    11461 taggcatgga aaagaatttt ctggctcgtg ttacagctat aatcagtgat tttaaaagga
    11521 atattagaac tgctcaacgt atggctaaaa ctgatatacc ggacgaaatc aagacagaag
    11581 ttacagcaaa cataagagac taccaaagag agctaacgcg agctaaatcg atggctcaac
    11641 gttggcgaga acataaagtt aatatcgatg cagacgctag caaagtaaaa caagtcatat
    11701 cgtttgttaa agcagaacta tcgaatatca gacgtaaaaa agttgaaatc gacggcgacg
    11761 caagcggatt aaaaagaaat gttgcgactt ctaaagcgat gttagctggt tggcgcaaac
    11821 acactgttaa attagatttt gatacaaccg gaatgtcgaa aatgcaagta gcattgactg
    11881 caggtaaaag agcattagat cagtatcaat caacaatgga tggcatcgca tcaaatatta
    11941 gaactttcgg tactatcttt gcgcaacaag tcaaaggttt aatgattgct agtatacaag
    12001 cattaatacc agtaattgct ggattagttc cggcaattat ggcagtactt aatgccgttg
    12061 gtgtattagg tggtggtgtt attgggcttg ctggtgcatt ctctgtagct ggtgttggag
    12121 cggttggctt cggtgcaatg gctattactg cactaaaaat ggtaaaagat ggaacgttag
    12181 cagtaacaaa agaagttcaa aattttagag atgcgagcga tcagttaaaa actacatggc
    12241 aaggcattgt aaaagagaat caagcaagta tctttaatgc gatgtcagcg ggtatcagag
    12301 gcgttacaag tgcgatgtct caattaaaac cattcttatc cgaagtatct atgctggttg
    12361 aagcaaacgc acgcgaattt gaggattggg ttaaacattc tgaaacaact aagaaagcat
    12421 ttgaagcatt gaatagcata ggtggcgcaa tcttcggaga tttattgaac gctgcaggac
    12481 gatttggcga cggattagtt aacattttca ctcaattaat gccgttgttc aaatttgtgt
    12541 ctcaaggact acagaacatg tctatagctt tccaaaattg ggctaatagt gtagctggtc
    12601 agaatgctat taaagcgttt attgactaca ctaccactaa cttacctaag attggtcaga
    12661 tatttggtaa tgtgttcgct ggtattggta atttaatgat tgcttttgca caaaacagtt
    12721 ccaacatttt tgattggttg gttaaattaa cttctcaatt tagagcatgg tcagaacaag
    12781 taggacaatc acaagggttt aaagacttta tcagttatgt tcaagagaat ggtcctacta
    12841 ttatgcagtt aatcggtaat atcgtaaaaa cattagttgc ttttggtact gcaatggctc
    12901 ctatagctag taaattgtta gactttatca ctaatctagc tggatttatc gctaaactat
    12961 tcgaaacaca cccagctata gcacaagttg ctggcgttat gggtatttta ggcggtgtat
    13021 tttgggcttt aatggctccg attgttgcta taagtagtgt acttacaaat gtgtttggtt
    13081 tgagcttatt cagcgtcact gaaaagattt tagacttcgt tagaacatca agtttagtta
    13141 ctggagctac ggaagcatta ataggtgcat tcggttcgat ttcagcacct attttagcag
    13201 ttgttgcagt aattggtgca ttcattggtg tcctcgttta tttatggaaa acaaacgaga
    13261 actttagaaa tactattact gaagcgtgga acggtgttaa aacggcagtt tctggtgcga
    13321 ttcaaggtgt agtcggctgg ttaactcaat tgtggggcaa aattcaatca acattacagc
    13381 caatcatgcc tatattacaa gtattaggac aagtattcat gcaagtttta ggtgttttgg
    13441 taataggcat tattacaaat gttatgaata tcatacaagg tttgtggacg ttaattacaa
    13501 ttgcgttcca agccatagga acagtgatat ccgtggcagt ccaaatcata gttggtttgt
    13561 tcactgcttt aattcagttg cttactggcg acttctcggg tgcttgggag actattaaaa
    13621 ctacggttac caatgtactt gatacgattt ggcaatacat gcaatcagtt tgggagtcaa
    13681 tcatcggctt tttaactggc gtaatgaatc gaacactttc tatgtttggt acaagttggt
    13741 cacagatatg gagtacaatc actaattttg ttagcagtat ttggaacagt gttacaagtt
    13801 ggtttagtcg tgttgcttcg agtgtggccg aaaaaatggg acaagcacta aactttatta
    13861 tcacaaaagg ttctgaatgg gtttctaata tttggaatac tgttacaagt ttcgcaagta
    13921 aagtagctga tggatttaaa agagttgtct caaatgtagg cgacggcatg aaaaacgcgc
    13981 ttgataagat taaaagcttt ttcagcgatt ttttaaatgc cggagcggaa ttaatcggca
    14041 aagtggcaga aggtgtagct aacgctgcgc acaaagtagt aagcgcggta ggcgatgcga
    14101 tttcatcagc gtgggactca gtaacttcat tcgtaagtgg acatggtgga ggtagtggtt
    14161 taggtaaagg tttagcggta tcacaagcta aagtaatggc tactagcttc ggtaaaacgt
    14221 tcacaagtga gttaggttca acgttgacag atggattcaa cgacagttta acaccaagcg
    14281 ttgacggcca tatgacaaac gatgtgcaac atagcatgaa agaaaataac agacctattg
    14341 ttaatgtaac tgttagaaac gagggcgatc taaacatgat taaatctcac attgacgata
    14401 tggatgcaaa agatggtagt ttcaacttaa tgtaagggag gtttgtttat tgatagccca
    14461 tgatgtagaa attattaaaa atggtgtgaa gtatcgcgtc agtgacaatc ctcacactta
    14521 caaacactta agagtgcttg attacaatgt tatcggttcg ggttacaaaa ggaattattc
    14581 gcctttagat ggagttgatg gacgttttca caattacgct aaagaagaat ataaaaaagt
    14641 tgaattaaga ttgaggtatg aagtacctaa gattgcttat gcctcacatc ttaaatcaga
    14701 cattcaaaca ttgttttatg gtcgctttta cctaagagaa ttggcgacgc cggataacac
    14761 tatcaaattt gaaaatatgt tcgaaccatt agaacaagaa tttgaattag attatgttga
    14821 cggtagacaa ctattcgttg gattagttag cgaagtatct tttgacacaa ctaagacatc
    14881 aggagaaatc acgttgactt ttgagacgac tgaattgcct ttctttgaaa gtattggcta
    14941 tagcactgat ttagaaagtg ataacgattt agaaaaatgg tcagttccgg acagaatagc
    15001 actaaatgaa aatgatagaa gtagacaaat gacattctat aacacgagtt ctggagatgt
    15061 ttattacaac ggagatgtag cattaacaca gttcaaccaa ttcaatgtag ttgaaattga
    15121 attagccgaa gatgttaaag ctgatgataa agacggtttc actttctata cggataaagg
    15181 aaacatctca gtaattaaag atgtcgattt aaaagcaggc gataaaatca tttttgataa
    15241 caagcacaca tatagaggca atttaaatat tgacctatac aacaaaacgt tagaacaacc
    15301 ggtgttgtat cccggttgga atcattttaa agccaacaga cttatgaaaa agatagtctt
    15361 tagacacaaa ttatattaca gataaggagt agcatatgcc ggtattatta aaaagtttgc
    15421 aaggcgtcgg tcatgcgatt catgttaata caaaattaaa cgaaaaattg aatgaagata
    15481 gcacgttaga cattgatatg atagaaaatg ccagcacttt cgacgcaatc ggcgctatta
    15541 caaagatgtg gactatcaca aacgtaaagg gggaagatga cctcaacgaa tatgtaatag
    15601 taatgcttga taaatcaaca atcggaaaca aaatcaaact tagtatcaaa gcgagacaaa
    15661 aagaattaga tgatctaaac aattctagga tttaccaaga atataacgaa agtttcacag
    15721 gcgtagagtt ttttaacact gtatttaaag gaactagtta taagtacgta ttgcacacta
    15781 aggttgacgc atcaaagttc gagggattag gcaagggaga cacaagactt gagatattca
    15841 aaaaaggact tgaacgctat catctcgaat atgagtacga cgctaaaaca aaaacatttc
    15901 acttgtatga cgaattatca aaagtagcaa actactatat taaatcaggt gtaaatgctg
    15961 ataacgtcaa aattcaagaa gatgcttcta aatgctacac atatataaga ggttatggcg
    16021 actttgacgg tcagcaaact tttacagagg ctgggttaca attcgaattc acacacccat
    16081 tagcacaact gattggtaaa agggaagcgc ctccgttaat agatggacgc ataaaaaaag
    16141 aagatgtttt gaaaaaatca atggagctag tgataaagaa aagtgtcact gcttctattt
    16201 ctttggactt cgtagcacag cctgagcatt ttccagaggc taaccctaga attggcgata
    16261 ttgtaagagt agctgaacca actataggct ataacgattt agtgagaata gtcgaaatca
    16321 ctacacatag agatgcgtac aataacatta ttaaacaaga tgtagtatta ggcgatttta
    16381 caatgcgtga cagatataga aaagctatcc atgaagctac gaactatgtt aagaatgtaa
    16441 aaacaactaa gtcagaccca gctaagtact tgagagaact aaacgctaaa gttaacgcta
    16501 gtttatctat aaataacgaa ttagttaagc agaatgaaaa aataaacgca aaagtcgata
    16561 agatgagtac taaaacagtt acaactgcga atggcacgat catgtacgac tttacgagtc
    16621 aatcaagtat aagaaatatc aaatctattg gaacgattgg cgattctgta gctagagggt
    16681 cgcatgcaaa aactaatttc acagaaatgt taggcaataa attaaaagcc aaaacaacaa
    16741 accttgcaag aggtggtgct acaatggcaa cagttccaat aggtaaagaa gcggtagaaa
    16801 acagcattta tagacaagca gagcaaataa gaggagacct aatcatatta caaggtacag
    16861 atgatgactg gttacatggt tattgggcag gcgtaccgat aggcactgat aaaacggata
    16921 ctaaaacgtt ttatggtgcc ttttgttctg caattgaagt tatacggaag aataatccgg
    16981 attcaaaaat actagtaatg acagctacta gacaatgtcc tatgcgtggt acaacgatac
    17041 gccgtaaaga tacggataaa aacaaactag ggttaacgtt agaggattat gtcaacgctc
    17101 aaatactagc ttgtagcgag ttggacgtac cagtgtttga cgcatatcac acagactact
    17161 ttaagccata caatccagct tttaggaaag cgagcatgga ggacggctta caccctaacg
    17221 aaaaaggtca cgaggttatt atgtacgagt taatcaagga ttattacagt ttttacgact
    17281 aaaggaggca accaatggct tacggattaa ttacaagttt acattcaatg acaggtcgga
    17341 aaatagttgc tcaacatgag tataactatc gcttgttaga tgaaggtatg agcaaacttg
    17401 agaaaatgtt tatataccat caaaaagaag aaatatacgc acactcagcg aaacaaatta
    17461 aatacttgaa tgacagtgtt gaagattatt taacgtattt aaatggccgt tttagcaata
    17521 tgattctagg ccataacggc gacggtatca atgaagtaaa agacgcgcgt attgataata
    17581 caggttatgg tcataagaca ttgcaagatc gtttgtatca tgattattca acactagatg
    17641 ctttcactaa aaaggttgag aaagttgtag atgaacacta taaagaatat cgagcgacag
    17701 aataccgatt cgaaccaaaa gagcaagaac cggaatttat cactgattta tcgccatata
    17761 caaatgcagt aatgcaatca ttttgggtag accctagaac gaaaattatt tatatgacac
    17821 aagcgcgtcc aggcaatcat tacatgttat ctagattgaa gcctaacgga caatttattg
    17881 atagactgct agttaaaaat ggcggacacg gcacacacaa cgcctataga tatatcggca
    17941 atgagttgtg gatttattca gcagtgttag acgctaacaa caataacaag tttgtacgct
    18001 ttaaatacag aagcggagaa atgacgtatg gcaacgaaat gcaagacgtt atgccaaatg
    18061 tatttaacga tagatatacg tcagcaattt ataatcctat agaaaactta atggttttta
    18121 gacgtgaata taaaacttct gaacaacaag ctaagaacgc attaaatttt gttgaagtaa
    18181 gaagtgctga cgatattgat aaaggtatag acaaagtact gtaccaaatg gatatcccta
    18241 tgcaatacac atcaggtacg caacctatgc aaggtattgc ttatgatgca ggtatcttat
    18301 attggtacac tggcgattca aatccagcta accctaatta cttacaaggc ttcgatatca
    18361 aaacgaaaga attgttattt aaacgtcgta tcgatatagg cggtgtgaat aacaacttta
    18421 aaggagattt ccaagaggct gagggtctag atatgtatta cgatttagaa acaggacgca
    18481 aagcgctttt aatcggggta actattggac ctggtaacaa cagacaccac tcaatttatt
    18541 caattggtca aagaggtgta aaccaattct taaaaaacat cgcacctcaa gtatcaatga
    18601 ctgattcagg tggacgtgtt aaaccgttac cagtgcaaaa cccagcatat ctaagtgata
    18661 ttacggaagt tggtcattac tatatctata cgcaagacac acaaaatgcg ttagatttcc
    18721 cgttaccgaa agcgtttaga gatgcaggtt ggttctttga tgtactgcct ggtcattata
    18781 atggtgcgtt aagacaagta ctaactagaa acagcacagg tagaaatatg ctcaaatttg
    18841 aacgtgttat cgacatcttt aacaagaaaa acaacggttc atggaatttt aacccacaaa
    18901 gtgctggtta ttgggaacat atccctaaga gcatcacgaa attgtctgat ttaaaaattg
    18961 ttggtttaga cttctatatc accactgaag aatcaaaacg tttttctgac ttccctaaag
    19021 attacaaagg tattgcaggc tgggtgttag aagtaaaatc aaatacaccg ggtaacacaa
    19081 cacaagtgct aagacgtaat aactttgctt ctgctcacca gtttttcgtt agaaactttg
    19141 gtactggtgg taatagtggt tggagcataa tagaaggtaa ggaggttgaa taatggtagt
    19201 agataatttt tcgaaagatg ataacttaat cgagttacaa acaacatcac aatataatcc
    19261 ggttattgac acaaacatca gtttctatga atcagataga ggaactggtg ttttaaattt
    19321 tgcagtaact aagaataaca gaccgttatc tataagttct gaacatgtca aaacatctat
    19381 cgtgttaaaa accgatgatt ataacgtaga tagaggcgct tatatttcag acgaattaac
    19441 gatagtagat gcaattgatg ggcgtttgca gtatgtgata ccgaatgaat ttttaaaaca
    19501 ttcgggtaag gtgcatgctc aagcattctt tacacaaaat gggagtaata atgttgttgt
    19561 tgaacgtcaa tttagcttca atatcgaaaa tgatttagtt agtgggtttg atggtataac
    19621 aaagcttgtt tatatcaaat ctattcaaga tactatcgaa gctgtcggta aagactttaa
    19681 ccaattaaag caaaatatgg ctgatacaca aacgttaata gcaaaagtga atgatagtgc
    19741 gacaaaaggc attcaacaaa tcgaaatcaa gcaaaacgaa gctatacaag ctattactgc
    19801 gacgcaaact agtgcaacac aagctgttac agctgaattc agtaaaatag ttgaaaagga
    19861 gcaagcgata tttgcgcgtg tcaatgaagt tgagaaacaa atcaatggtg ctgaccttgt
    19921 caaaggtaac acaacgacaa attggcaaaa atcaaaaatt actgatgatt atggtaaagc
    19981 aattgaatcg tctgaacagt ccatagatag cgttttaagc gcaattaata catctaggat
    20041 tattcatatc actagcgcga cagatgcgcc ctcgtttaaa gatataggca ctttagagac
    20101 gcctaaagaa gatggcgttg atgatggttc tgaagtttca gcaactacga atactttagg
    20161 gaaatcaggc ttgttagttg tctatgttgt tgatgatagt acggcacgtg caacatggta
    20221 tccagacgat tcaaatgatg agtacacaac atataaaatc ggtggcacat ggtatcagtt
    20281 ctataaaaaa gttgacgaag aattaacgaa gaaatttgtt aaagaaacat ctaacaatgc
    20341 tttaaatcaa gctaagcagt atgtagatga taaattcgga acaacgagtt ggcaacaaca
    20401 taagatgaca gaggcgaacg gtcaatcaat acaagttaac ttaaacaatg cgcaaggcga
    20461 tttaggctat ttaactgctg gtaattacta tgcaacaaga gtgccggatt taccaggtag
    20521 tgttgaaagt tatgagggtt atttatctgt attcgttaaa gatgaaacaa acaaattttt
    20581 caactttacg ccttataact ctaaaaagat ttacacacga tcaatcacaa acggcagact
    20641 tgagcaacag tggacagttc ctaatgaaca taagtcaacg gtattgttcg acggtggagc
    20701 aaatggtgta ggtacaacaa tcaatctaac cgaaccatac acaaactatt ctattttatt
    20761 agtaagtgga acttatccag gtggcgttat tgagggattc ggactaacca cattacctaa
    20821 tgcaattcaa ttaagtaaag cgaatgtagt tgactcagac ggtaacggtg gcggtattta
    20881 tgagtgttta ctatccaaaa caagtagcac tactttaaga atcgataacg atgtgtactt
    20941 tgatttaggt aaaacatcag gttctggagc gaatgccaac aaagttacta taactaaaat
    21001 tatggggtgg aaataatgaa aatcacagta aacgataaaa acgaagttat cggatacgtt
    21061 aatactggcg gtttacgtaa tagtttagat gtagacgata acaatgtgcc tatcaaattc
    21121 aaagaagagt tcgaacctag aaagtttgtt ttcactaacg gcgaaattaa atacaatagc
    21181 aatttcgaaa aagaagacgt accgaatgca tcaaaccaac aaagtgcgtc agatttaagt
    21241 gatgaggaac ttcgcggaat ggttgcgagt atgcaaatgc agatgacgca agtgaacatg
    21301 ttgacaatgc aattgacgca acaaaacgct atgttaacac aacagttgac cgaactgaaa
    21361 actaacaaaa caaatactga gggggacgtt taaatgatga agatgattta tccaactttt
    21421 aaagacatta aaacttttta tgtgtggggt tgctataaaa atgagcaaat taagtggtac
    21481 gtagacatgg gtgtaatcga caaagaagaa tatgcattga tcactggtga aaaatatcca
    21541 gaggcaaaag atgaaaagtc acaggtgtaa tgcttgaggc tttttaattt aacacaaagt
    21601 aggtggcgta atgtttggat ttaccaaacg gcacgaacat gaatggcgaa ttagaagatt
    21661 agaagagaat gataaaacaa tgcttagcac tctcaatgag attaaattag gtcaaaaaac
    21721 tcaagagcaa gttaacatta aattagataa aactttagat gctatccaga gggaaagaca
    21781 gatagacgaa aaaaataaga aagaaaacga caaaaatata cgcgatatga aaatgtggat
    21841 tctcggtttg atagggacta tcttcagtac gattgtcata gctttactaa gaactatttt
    21901 tggtatttaa aggaggtgat taccatgctt aaagggattt taggatatag cttctgggcg
    21961 tgcttctggt ttggtaaatg taaataacag ttaagagtca gtgcttcggc actggctttt
    22021 tattttgatt gaaatgaggt gcatacatgg gattacctaa cccaaagact agaaagccta
    22081 cagctagtga agtggtggag tgggcaaagt cgaatattgg taagaggatt aatatagata
    22141 attatcgggg cagtcaatgt tgggatacac ctaactttat ttttaaaaga tattggggtt
    22201 ttgtaacatg gggcaatgct aaggatatgg ctaattacag atatcctaag ggtttccgat
    22261 tctatcgtta ttcatctgga tttgtaccgg aacctggaga catcgcagtt tggcaccctg
    22321 gcaacggaat aggttcggac ggacacaccg caatagtagt aggaccatct aataaaagtt
    22381 atttttatag cgttgaccaa aactgggtta attctaatag ttggacaggt tctccaggaa
    22441 gattagtaag acacccttat gtaagtgtta caggctttgt taggcctcca tactcaaaag
    22501 atactagcaa acctagtagt actgatacaa gttcagcatc aaaagccaat gactcaacaa
    22561 ttactggcga agcgaagaaa ccgcaattta aagaagttaa aacagtaaaa tacactgctt
    22621 acagcaatgt tttagataaa gaagagcact tcattgatca tatagttgta atgggtgatg
    22681 aacgctcaga tattcaagga ttatatataa aagaatcaat gcatatgcgt tctgtagacg
    22741 aactgtatac gcaaagaaat aagtttataa gcgattatga aataccgcat ttatatgtcg
    22801 atagagaggc tacatggctt gctagaccaa ccaattttga tgacccgcgt caccctaatt
    22861 ggctagttat tgaagtatgt ggtggtcaaa cagatagcaa acgacaattc ttattgaatc
    22921 aaatacaagc gttaatacgt ggtgtttggt tattgtcagg gattgataaa aacttatctg
    22981 aaacgacgtt aaaggtagac cctaatattt ggcgtagtat gaaagattta attaattacg
    23041 acttgattaa gcaaggtata ccggataacg caaagtatga gcaagttaaa aagaaaatgc
    23101 ttgagacata cattaaacga gatatattga cacgagaaaa tataaaagaa gtaacgacaa
    23161 aaacaacaat aagaattagt gataaaacat cagttgacag tgcgtccaca cgaggcccta
    23221 ctccatcaga cgaaaaacca agcatcgtta ctgaaacaag tccattcaca ttccagcaag
    23281 cactggatag acaaatgtct aggggtaacc cgaaaaaatc tcatacatgg ggctgggcta
    23341 atgcaacacg agcacaaacg agctcggcaa tgaatgttaa gcgaatatgg gaaagtaaca
    23401 cgcaatgcta tcaaatgctt aatttaggca agtatcaagg catttcagtt agtgcgctta
    23461 acaaaatact taaaggaaaa ggaacgctcg acggacaagg caaagcattc gcggaagctt
    23521 gtaagaaaaa caacgttaac gaaatttatt tgatcgcgca cgctttctta gaaagtggat
    23581 acggaacaag taacttcgct aatggtagat acggtgcata taattacttc ggtattggtg
    23641 cattcgacaa cgaccctgat tatgcaatga cgtttgctaa aaataaaggt tggacatctc
    23701 cagcaaaagc aatcatgggc ggtgctagct tcgtaagaaa ggattacatc aataaaggtc
    23761 aaaacacatt gtaccgaatt agatggaatc ctaagaatcc agctacccac caatacgcta
    23821 ctgctataga gtggtgccaa catcaagcaa gtacaatcgc taagttatat aaacaaatcg
    23881 gcttaaaagg tatctatttt ataagagata aatataaata aagaggtgta taaatgtaca
    23941 aaataaaaga tgttgaaacg agaataaaaa atgatggtgt tgacttaggt gacattggct
    24001 gtcgatttta cactgaagat gaaaatacag catctataag aataggtatc aatgacaaac
    24061 aaggtcgtat cgatctaaaa gcacatggct taacacctag attacatttg tttatggaag
    24121 atggctctat attcaaaaat gagcccctta ttatcgacga tgttgtaaaa gggttcctta
    24181 cctacaaaat acctaaaaag gttatcaaac acgctggtta tgttcgctgt aagctgtttt
    24241 tagagaaaga agaagaaaaa atacatgtcg caaacttttc tttcaatatc gttgatagtg
    24301 gtattgaatc tgctgtagca aaagaaatcg atgttaaatt ggtagatgat gctattacga
    24361 gaattttaaa agataacgcg acagatttat tgagcaaaga ctttaaagag aaaatagata
    24421 aagatgtcat ttcttacatc gaaaagaatg aaagtagatt taaaggtgcg aaaggtgata
    24481 aaggcgaacc gggacaacct ggtgcgaaag gtgatacagg taaaaaagga gaacaaggcg
    24541 cacccggtaa aaacggtact gtagtatcaa tcaatcctgg cactaaaatg tggcaaattg
    24601 atggtaaaga tacaaatatc aaagcagaac ctgagttatt ggacaaaatc aatatcgcaa
    24661 atgttgaagg gttagaagat aaattgcaag aagttgaaaa aatcaaagat acaactctca
    24721 acgactctaa aacgtatacg gattcaaaaa ttgctgaact agttgatagc gcgcctgaat
    24781 ctatgaatac attaagagaa ttagcagaag caatacaaaa caactctatt tcagaaagtg
    24841 tattgcaaca gattggctca aaagttagta cagaagattt tgagagattc aagcaatcat
    24901 taaacagttt gtatgcagat aaaaatcata gtcatacaat caaacagatt gaaggattag
    24961 aaaatgcttt atcaaaaaaa tcagacataa atcacagtca tgatgaacgt tatcttttat
    25021 catcaaatgc ttttacaaaa gaggaagcag ataaacttta tcaacctatc ggttcttcgc
    25081 agccgtcact gaatatttgg acaggcagtg aaacagaata taattatttg tatcaaaaag
    25141 accctaatac actttactta attaaggggt gattttatgg aaggtaattt taaaaatgta
    25201 aagaagctta tttacgaagg cgaagaatat acaaaagtat atgctggaaa tatccaagta
    25261 tggaaaaaac cttcatcttt tgtaataaaa cccttaccta aaaataaata tccggatagc
    25321 atagaagaat caacagcaaa atggacaata aatggagttg aacctaataa aagttatcag
    25381 gtgacaatag aaaatgtacg tagcggtata atgagggttt cgcaaactaa tttagggtca
    25441 agtgaattag gaatatcagg agtcaatagc ggagttgcaa gtaaaaatat caactttagt
    25501 aatccttcag ggacgttgta tgtcactata agtgatgttt attcaggatc tccgacattg
    25561 accattgaat aattttaaac gactaatttt ttagttgttt tttattttgg ataaaaggag
    25621 caaacaaatg gatattaact ggaaattgag attcaaaaat aaagcagtac taactggttt
    25681 agttggagcg ttgttgctat ttatcaagca aatcacggat ttgttcggat tagatttatc
    25741 cactcaatta aatcaagcta gcgctattat aggcgctatc ctcacgttac ttacaggtat
    25801 tggtgttatt actgacccaa cgtcaaaagg cgtctcagat tcatctatag cacagacata
    25861 tcaagcgcct agagatagca aaaaagaaga acaacaagtt acgtggaaat catcacaaga
    25921 cagtagttta acgccggaat taagcgcgaa agcaccaaaa gaatatgata catcacaacc
    25981 tttcacagac gcctctaacg atgttggctt tgatgtgaat gagtatcatc atggaggtgg
    26041 cgacaatgca agcaaaatta actaaaaaag agtttataga gtggttgaaa acttctgagg
    26101 gaaaacaatt caatgtggac ttatggtatg gatttcaatg ctttgattat gccaatgctg
    26161 gttggaaagt tttgtttgga ttacttctaa aaggtttagg tgcaaaagat ataccatttg
    26221 caaacaattt tgatggacta gctactgtat accaaaatac gccggacttt ttggcaaaac
    26281 ccggcgatat ggttgtgttc ggtagtaatt acggtgcagg atacggacac gtagcatggg
    26341 taattgaagc aactttagat tatatcattg tatatgagca gaattggcta ggcggtggct
    26401 ggactgacgg aatcgaacaa cccggctggg gttgggaaaa agttacaaga cgacaacatg
    26461 cttatgattt ccctatgtgg tttatccgtc cgaattttaa aagtgagaca gcgccacgat
    26521 cagttcaatc tcctacacaa gcacctaaaa aagaaacagc taagccacaa cctaaagcag
    26581 tagaacttaa aatcatcaaa gatgtggtta aaggttatga cctacctaag cgtggtagta
    26641 accctaaagg tatagttata cacaacgacg cagggagcaa aggggcgact gctgaagcat
    26701 atcgtaacgg attagtaaat gcacctttat caagattaga agcgggcatt gcgcatagtt
    26761 acgtatcagg caacacagtt tggcaagcct tagatgaatc acaagtaggt tggcataccg
    26821 ctaatcaaat aggtaataaa tattattacg gtattgaagt atgtcaatca atgggcgcag
    26881 ataacgcgac attcttaaaa aatgaacagg caactttcca agaatgcgct agattgttga
    26941 aaaaatgggg attaccagca aacagaaata caatcagatt gcacaatgaa tttacttcaa
    27001 catcatgccc tcatagaagt tcggttttac acactggttt tgacccagta actcgcggtc
    27061 tattgccaga agacaagcgg ttgcaactta aagactattt tatcaagcag attagggcgt
    27121 acatggatgg taaaataccg gttgccactg tctctaatga gtcaagcgct tcaagtaata
    27181 cagttaaacc agttgcaagt gcatggaaac gtaataaata tggtacttac tacatggaag
    27241 aaagtgctag attcacaaac ggcaatcaac caatcacagt aagaaaagtg gggccattct
    27301 tatcttgtcc agtgggttat cagttccaac ctggtgggta ttgtgattat acagaagtga
    27361 tgttacaaga tggtcatgtt tgggtaggat atacatggga ggggcaacgt tattacttgc
    27421 ctattagaac atggaatggt tctgccccac ctaatcagat attaggtgac ttatggggag
    27481 aaatcagtta gaatgacata gtcatgtcta tttaagcagg tgcgttacac acctgctttc
    27541 tatttacatt taaagataaa atgtgctatt attttactag aactttttaa catttctctc
    27601 aagatttaaa tgtagataac aggcaggtac tacggtactt gcctattttt tatgttataa
    27661 tgtaattaca ttaccagtaa ccaatctggc ttaaaaccac atttccggta gccaatccgg
    27721 ctatgcagag gacttacttg cgtaaagtag taagaagctg actgcatatt taaaccaccc
    27781 atactagttg ctgggtggtt tttatgttat aatataaatg tgaaatggtc attcttgaaa
    27841 tgactcggtc gctactggca cagactgttt aaagtgtcgc cacaacatga actgagaatt
    27901 catatgacgt tgctgacgag cgacaaagct ctgtgttcct gaacgggagt aggtttgtgt
    27961 ggtggtttaa tttagtaaca gcatagactg tctatagcaa ggttgccgaa gagattctaa
    28021 acgtattcgt aagtacgtgg tacttgctag ataaccgtat cttaaccgat gcggttattt
    28081 tttaccacac aaccaacaaa accacaccac ctattaattt aggagtgtgg ttgtttttgt
    28141 tggaagtgtg tat
//
LOCUS       DANBWH010000011        26640 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.011, whole genome shotgun sequence.
ACCESSION   DANBWH010000011 DANBWH010000000
VERSION     DANBWH010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 26640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26640
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.011"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(47..511)
                     /gene="bstA"
                     /locus_tag="PPY92_000320"
     CDS             complement(47..511)
                     /gene="bstA"
                     /locus_tag="PPY92_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol transferase BstA"
                     /protein_id="HDJ2624348.1"
                     /translation="MTTKTVFDVIDMGLGYLVNVYDAWKVEKVLDDYHEPFSNTIHWQ
                     FGHVLTIFESALAVAGKENIDLNIYRPLFGNGSSPDEWKDEVPSIEKILEGLQTLPER
                     ARNLTEDDLAIELKQPIVGCNNLEELLVLNAIHIPLHAGKIEEMSRILKNLK"
     gene            678..1016
                     /locus_tag="PPY92_000321"
     CDS             678..1016
                     /locus_tag="PPY92_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDJ2624349.1"
                     /translation="MSVSDQIMKGLLDGAILGLIAQGETYGYEIMTKLKNQEFPEISE
                     GSIYPVLMRLNKKGYVDTTIKKSSNGGPRRKYYTITDSGLKELKVFKSKWQALNKGMI
                     NLFGGENDVN"
     gene            1006..1773
                     /locus_tag="PPY92_000322"
     CDS             1006..1773
                     /locus_tag="PPY92_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624350.1"
                     /translation="MLTKQSQDFLEKLRVELLFRGKTEAEVEELLEELEDHLTMAEKN
                     NEDTSSIINTPIKSMADQLSPEISLTTGLYKYITLYFSFILAVIIIPRFLDLGTFDVT
                     IAFLLYIASIVILGVVIGMLILRNTLVRFGDQKITYVINVTYGIVVFLWMIFGGLIIK
                     KYPIYSFFELSDKQSFIVGLVLLIALIVICILIKQKIYALIVLVISLPSLIARIAAIF
                     SKEPDTFNLVSVSILIILNIAFIIYTFVNFRKLKKED"
     gene            complement(1892..2530)
                     /gene="pcp"
                     /locus_tag="PPY92_000323"
     CDS             complement(1892..2530)
                     /gene="pcp"
                     /locus_tag="PPY92_000323"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDJ2624351.1"
                     /translation="MHILVTGFAPFDNQNINPSWEAVTQLEDIIGTHTIDKLKLPTSF
                     KKVDNIINKTLASNHYDVVLAIGQAGGRNAITPERVAINIDDARIPDNDDFQPIDQAI
                     HLDGAPAYFSNLPVKAMTQSIINQGLPGALSNSAGTYVCNHVLYHLGYLQDKHYPHLR
                     FGFIHVPYIPEQVIGKPDTPSMPLEKIVAGLTAAIEAISNDEDLRIALGTTE"
     gene            complement(2682..3627)
                     /locus_tag="PPY92_000324"
                     /pseudo
     CDS             complement(2682..3627)
                     /locus_tag="PPY92_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501473.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
     gene            3871..4842
                     /locus_tag="PPY92_000325"
     CDS             3871..4842
                     /locus_tag="PPY92_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HDJ2624352.1"
                     /translation="MSQQDLPTLFYSGKSNSAVPIISESELQTITAEPWLEISKKGLQ
                     LEGLNFDRQGQLFLLDVFEGNIFKINPETKEIKRSFVSHKANPAAIKIHKDGRLFVCY
                     LGDFKSTGGIFAATENGDNLQDIIEDLSTAYCIDDMVFDSKGGFYFTDFRGYSTNPLG
                     GVYYVSPDFRTVTPIIQNISVANGIALSTDEKVLWVTETTANRLHRIALEDDGVTIQP
                     FGATIPYYFTGHEGPDSCCIDSDDNLYVAMYGQGRVLVFNKRGYPIGQILIPGRDEGH
                     MLRSTHPQFIPGTNQLIICSNDIEMGGGSMLYTVNGFAKGHQSFQFQ"
     gene            complement(4993..5508)
                     /locus_tag="PPY92_000326"
     CDS             complement(4993..5508)
                     /locus_tag="PPY92_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HDJ2624353.1"
                     /translation="MTNFTFDGAHSSLEFQIKHLMVSKVKGSFDQFDVAVEGDINDFS
                     TLKATATIIPSSINTKNEARDNHLKSGDFFGTDEFDKITFETKSVTENKVVGDLTIKG
                     ITNEETFDVEFNGVSKNPMDGSQVTGVIVTGTINRENYGINFNQALETGGVMLGKDVK
                     FEASAEFSISE"
     gene            5887..6725
                     /locus_tag="PPY92_000327"
                     /pseudo
     CDS             5887..6725
                     /locus_tag="PPY92_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501470.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
     gene            6754..7308
                     /locus_tag="PPY92_000328"
     CDS             6754..7308
                     /locus_tag="PPY92_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HDJ2624354.1"
                     /translation="MKKQDISVKTVVAIGIGAAVFVILGRFVVIPTGFPNTNIETSYA
                     FLALISAIFGPFAGLMTGLVGHAIKDFTTYGSAWWSWVICSGIIGCLYGWIGLKLNLS
                     SGLFSRKSMIYFNIGQIIANIICWALIAPTLDILIYNEPANKVYTQGVISAVLNIISV
                     GIIGTILLKAYASSQIKKGSLRKE"
     gene            7361..9073
                     /locus_tag="PPY92_000329"
     CDS             7361..9073
                     /locus_tag="PPY92_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDJ2624355.1"
                     /translation="MTEPIISFKDFSFQYHSQATPTLQNINVDIYPGEKVLVVGASGS
                     GKSTFANCINGLIPFKTKGNITGELYINNQDATVSCLHDRSNVVGTVLQDTDGQFIGL
                     TAAEDMAFLLENNCVEQDDMKKNVSYWAEKVGMIEHLNHRPQDLSGGQKQRVSLGGIL
                     IHRTPILILDEPLANLDPATGHETLRLLNNIHEETKSTMIIVEHRLEESLDDTFDRVL
                     LFKDGKIIANTTPSDLLKSSKLKEAGIREPLYCTALKYAEVDVESIDNLANLRDVCMS
                     EHVKFKVKKWIDETSANNDNKYKSEPLLELNEVCVQYSDYSNSVLNNVQLNVYRREML
                     SIVGHNGAGKSTLAKAICGFLDITGNIQFCNRGFNQLSISERSEFVGYVMQNPNHMIS
                     EKMIYDEVALGLRARGMKESDIKIRVENVLKICGLYAFRNWPIAALSYGQKKRVTIAS
                     VLVLNPEIIILDEPTAGQDFYHYNEIMSFLIELNRQGKTIIMITHDMHLLSEYSSRTV
                     VLSKGQVVADTTPVLILNDKKICEIASLRQTSLFEMAEYIGISEPQKLVQLFINHDRK
                     VRRQ"
     gene            9070..9903
                     /locus_tag="PPY92_000330"
     CDS             9070..9903
                     /locus_tag="PPY92_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDJ2624356.1"
                     /translation="MNQYNTIGFHPGNSRIHQLNATVKLLFLLVVSISAMVTYDTRYL
                     ILISASSILLFKYAHIEWKQVRFVVKFILFFTILNIIAVYIFDPEYGVKIYNQRTELV
                     NGIGRFTLTSQELFYLFNLILKYISTVPLALIFLFTTNPSHFAASLNQLGVNYKISYA
                     VSLALRYIPDIQETYFNISQAQQARGYDNSKKAKFTSRVKGIKRIVLPLIFSSIERID
                     TISTAMELRQFGQYKRRTWYVKKQLKKDDYVVLCLTLILLMLVVTLFFLNNSRYFNPW
                     H"
     gene            9950..10447
                     /locus_tag="PPY92_000331"
     CDS             9950..10447
                     /locus_tag="PPY92_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDJ2624357.1"
                     /translation="MINTERLNLMIPSSSHLIELYNICSHPQANIYTPKGLHNSKLDT
                     QRWIEKWQNHWQQYQFGYFVLVKKIDCSVIGICGYEYRQLKQETVLNLFYKLHPSFEG
                     QGYACEAITAITNFVNYIDQETVKVIRTNKCNQRSINLAERLKFKRDKAMDDIINQGD
                     IVFYK"
     gene            10565..11689
                     /locus_tag="PPY92_000332"
     CDS             10565..11689
                     /locus_tag="PPY92_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDJ2624358.1"
                     /translation="MHKKYLLLFFLLLSVALLYSCSKQNEKAQNKSVNPNEYAKHKSK
                     MDEHWETYHGQVYHVFYHPLITDPKVAFTGDTHQAKGNNDWMITVSEFKKSLNELYKN
                     NYIIVNPHDVYDLSSHHVKAKKIKLPKGKKPLIISIDDMNYYSYMRHHGYADRLVLDK
                     NKHVVSETTDKKGHATYSDDNDIVPILNKFVKEHPDFSLNGEKGVVALTGYEGVLGYR
                     TNELTSKDYTKNKKAAEEVVRAMKRDGWSFASHSYGHINFEKTSLEGIKRDTKRWKDE
                     VEPIVGKTDMFVFPHGAQDRHTQAYDYLVDEAEFKFIAGVGPNNFTDISATNVYQDRV
                     AIDGLNLFEFKYKLKPFFNPENVYSKQDRQFFKGNRDYEE"
     gene            11993..12811
                     /locus_tag="PPY92_000333"
     CDS             11993..12811
                     /locus_tag="PPY92_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HDJ2624359.1"
                     /translation="MNNSEQLIALKESETAFLKYFNKADYELVDFSVVEKLDWKQLNH
                     EDLQQMGERNFWQHEHQIYALRNDFTDQLLRYYSMYPTAATKVAYTGLIIRNNEAAVQ
                     VGLENYAPSLANVQQSLKLFIQFIQQQLRDNVHFVVLGHYQLLDALLDKSLQTPDILS
                     MIEERNLSGLVTYLSTEHPIVQILKENTQQQLNVLEHYIPNDHPALVELKIWERWLHT
                     QGYKDIHLDITAQPPRSYYTGLFIQCHFAENESRVLTGGYYKGSIEGFGLGLTL"
     gene            12829..13443
                     /locus_tag="PPY92_000334"
     CDS             12829..13443
                     /locus_tag="PPY92_000334"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDJ2624360.1"
                     /translation="MLRIAIAKGRLMDSLINYLDVIEYTTLSETLKNRERQLLLSVDN
                     IECILVKGSDVPIYVEQGMADIGIVGSDILDERQYNVNNLLNMPFGACHFAVAAKPET
                     TNYRKIATSYVHTAETYFKSKGIDVELIKLNGSVELACVVDMVDGIVDIVQTGTTLKA
                     NGLVEKQHISDINARLITNKAAYFKKSQLIEQFIRSLEVSIANA"
     gene            13436..14686
                     /locus_tag="PPY92_000335"
     CDS             13436..14686
                     /locus_tag="PPY92_000335"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDJ2624361.1"
                     /translation="MLNAQQFLNQFSLEAPLDESLYPIIRDICQEVKVHGDKALKMYN
                     LTFDHTKTDHLEISHEQIKAAFDTLDEKTKQALQQSYERIKAYQESIKQTNQQLEESV
                     ECYEIYHPLESVGIYVPGGKASYPSTVLMTATLAQVAGVENIVVVTPPQPNGVSQEVL
                     AACYITQVNQVFQVGGAQSIAALTYGTETIPKVDKIVGPGNQFVAYAKKYLFGQVGID
                     QIAGPTEIALIIDDTADLDAIVYDVFAQAEHDELARTYVIGEDAQVLKDLESRIAKAL
                     PNVDRYDIVSKSIANQHYLIHASNFDEACHVMNTIAPEHASIQTVNPQPYIEKVKYVG
                     ALFIGHYSPEVIGDYVAGPSHVLPTNRTARFTNGLSVNDFLTRNTVIHLSKDTFEQIA
                     DSAQHIAHVEALYNHQQSILIRQS"
     gene            14702..15715
                     /locus_tag="PPY92_000336"
     CDS             14702..15715
                     /locus_tag="PPY92_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HDJ2624362.1"
                     /translation="MIYIDKNESPVTPLDEKTMTSIISATPYNLYPDAAYEQFKEAYA
                     KFYGLSPEQIIAGNGSDELIQKLMLIMPEGPALTLNPDFFMYQAYAAQVNREIAFVDA
                     GSDLTFDLETILTKIDEVQPSFFIMSNPHNPSGKQFDTAFLTAIADKMKALNGYFVID
                     EAYLDYGTAYDVELAPHILRMRTLSKAFGIAGLRLGVLISTAGTIKHIQKIEHPYPLN
                     VFTLNIATYIFRHREETRVFLTMQRQLAEQLKQIFDTHVADKMSVFPSNANFVLTKGS
                     AAHQLGQYVYEQGFKPRFYDEPVMKGYVRYSIATASQLKQLEEIVKEWSAKYDLSKTT
                     KHS"
     gene            15684..16262
                     /gene="hisB"
                     /locus_tag="PPY92_000337"
     CDS             15684..16262
                     /gene="hisB"
                     /locus_tag="PPY92_000337"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HDJ2624363.1"
                     /translation="MIYQKQRNTAETQLNISISDDQSPSHINTGVGFLNHMLTLFTFH
                     SGLSLNIEAQGDIDVDDHHVTEDIGIVIGQLLLEMIKDKKHFVRYGTMYIPMDETLAR
                     VVVDISGRPYLSFNASLSKEKVGTFDTELVEEFFRAVVINARLTTHIDLIRGGNTHHE
                     IEAIFKAFSRALGIALTATDDQRVPSSKGVIE"
     gene            16259..16837
                     /gene="hisH"
                     /locus_tag="PPY92_000338"
     CDS             16259..16837
                     /gene="hisH"
                     /locus_tag="PPY92_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDJ2624364.1"
                     /translation="MIVIVDYGLGNISNVKRAIEHLGYEVVVSNTSKIIDQAETIILP
                     GVGHFKDAMSEIKRLNLNAILAKNTDKKMIGICLGMQLMYEHSDEGDASGLGFIPGNI
                     SRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGADIPAIVQF
                     NNYIGIQFHPEKSGTYGLQILRQAIQGGFIND"
     gene            16830..17534
                     /locus_tag="PPY92_000339"
     CDS             16830..17534
                     /locus_tag="PPY92_000339"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDJ2624365.1"
                     /translation="MIELWPAIDLIGSTSVRLTEGKYDSEEKMSRSAEESIAYYSQFE
                     CVNRIHIVDLIGAKAQYAREFDYIKSLRRLTTKDIEVGGGIRTKSQIMDYFAAGINYC
                     IVGTKGIQDTDWLKEMAHTFPGRIYLSVDAYGEDIKVNGWEEDTELNLFSFVRQLSDI
                     PLGGIIYTDIAKDGKMSGPNFELTGQLVKATTIPVIASGGIRHQQDIQRLASLNVHAA
                     IIGKAAHQASFWEGLE"
     gene            17531..18289
                     /gene="hisF"
                     /locus_tag="PPY92_000340"
     CDS             17531..18289
                     /gene="hisF"
                     /locus_tag="PPY92_000340"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDJ2624366.1"
                     /translation="MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAIYYNEAGA
                     DELVFLDISKTEEGHSLMLEVIEQTASRLFIPLTVGGGIQSLDDITQLLNHGADKVSL
                     NSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKAHYCCTHGGKKMTNIKVYDWVQ
                     QVEQLGAGELLVTSMGHDGMKQGFDIEHLAKIKSLVNIPIIASGGGGNAQHFVELFNQ
                     TDVSAGLAASILHDRETTVQSIKEVIRQGGIAVR"
     gene            18286..18918
                     /locus_tag="PPY92_000341"
     CDS             18286..18918
                     /locus_tag="PPY92_000341"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDJ2624367.1"
                     /translation="MTKYKIDFSKGLVPAILQDNQTKQVLMLGYMNQEAFDKTIEDGV
                     VCFYSRSKQRLWTKGETSGHTQLVKDIHVDCDNDTILIDVIPNGPTCHTGSQSCFNTE
                     VPFSVQTLAQTVQDSAQSNNEKSYTKYLLTEGIEKITKKYGEEAFEVVIEAIKGDKKA
                     FVSEVADELYHLFVLMHALGVDFSEIEAELARRHHKRNNFKGERQNIEQW"
     gene            19111..19290
                     /locus_tag="PPY92_000342"
     CDS             19111..19290
                     /locus_tag="PPY92_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624368.1"
                     /translation="MKSKLSNSGIDVTVKDVEKYMNRYNEVMMGKNGEKAKELCLSLL
                     PINIIVVFTFFVFIL"
     gene            20011..22053
                     /gene="lip1"
                     /locus_tag="PPY92_000343"
     CDS             20011..22053
                     /gene="lip1"
                     /locus_tag="PPY92_000343"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip1"
                     /protein_id="HDJ2624369.1"
                     /translation="MKSQNKYSIRKFSVGASSILIATLLFLSGGQAQAAEKQVNMGNS
                     QEDTVTAQSIGDQQTRENANYQRENGVDEQQHTENLTKNLHNDKTISEENHRKTDDLN
                     KDQLKDDKNSSLNNKNIQRDTTKNNNANPSDVNQGLEQAINDGKQSKVASQQQSKEVD
                     NSQDSNANNNLPSQSLTKEAPSLNKSDQTSQREIVNETEIEKVQPQQNNQANDKITNH
                     NFNNEQEVKPQKDEKTLSVSDLKNNQKSPVEPTKDNDKKNGLNLLKSSAVATLPNKGT
                     KELTAKAKDDQTNKVAKQGQYKNQDPIVLVHGFNGFTDDINPSVLAHYWGGNKMNIRQ
                     DLEENGYKAYEASISAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYEGI
                     YKDWKPGQKVHLVGHSMGGQTIRQLEELLRNGNREEIEYQKKHGGEISPLFKGNNDNM
                     ISSITTLGTPHNGTHASDLAGNEALVRQIVFDIGKMFGNKNSRVDFGLAQWGLKQKPN
                     ESYIDYVKRVKQSNLWKSKDNGFYDLTREGATDLNRKTSLNPNIVYKTYTGEATHKAL
                     NSDRQKADLNMFFPFVITGNLIGKATEKEWRENDGLVSVISSQHPFNQAYTNATDKIQ
                     KGIWQVTPTKHDWDHVDFVGQDSSDTVRTREELQDFWHHLADDLVKTEKVTDTKQA"
     gene            complement(22388..23440)
                     /gene="icaC"
                     /locus_tag="PPY92_000344"
     CDS             complement(22388..23440)
                     /gene="icaC"
                     /locus_tag="PPY92_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide intercellular adhesin
                     biosynthesis/export protein IcaC"
                     /protein_id="HDJ2624370.1"
                     /translation="MKKIRLELVYLRAIICAIIIITHLLTQITLKHENMEGGSLVLQF
                     YIRNIVIFGTPCFIILSQLLTTLNYQKVTYRYLTTRVKYILIPYILMGLFYSYSESLL
                     TDSSFNKQFIENVLLGQWYGYFIVVIMQFFILSYIIFKINYNLFNSKILLLLSFILQQ
                     SFLYYFTNNTAFHDTVLHYYPLSENTIIFGWIFYFFLGAYMGYNYERVLNFLERYLVI
                     MIVLAVATYFVFIALANGDYWNVTSFSYSLTPYNSIMFIVILGICTHFKTMLFNTIQM
                     ISAFSFFIYLLHPIILDSLFAYTNIFEDNTMVFLAISLLFILGLCIGVGMILREFYIF
                     RFIIGKQPYKLNINAY"
     gene            complement(23427..24299)
                     /gene="icaB"
                     /locus_tag="PPY92_000345"
     CDS             complement(23427..24299)
                     /gene="icaB"
                     /locus_tag="PPY92_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intercellular adhesin biosynthesis
                     polysaccharide N-deacetylase"
                     /protein_id="HDJ2624371.1"
                     /translation="MKYRKLIILVLSILIILPVSTLDGHHIANADDDSPKKLKYKENS
                     ALALNYHRVRKANFLNNFIYFFSSSKEIKNYSVSQSQFESQIKWLKSHDAKFLTLKEF
                     LYYKKKGKFPKRSVWINFDDMDETIYENAYPILKKYKIPATGFIITGHVGEENFHNLD
                     MISKKELKEMYKTGLWEFETHTHDLHNLSKNNKSKLMKASEATIIKDLNKSEKYLTKN
                     FKKSQKTIAYPYGLMNDDKLPVIKKAGLKYGFSLEEKAVTPNSNDYYIPRILISDDAF
                     EHLIKRWDGFHEKD"
     gene            complement(24296..24601)
                     /gene="icaD"
                     /locus_tag="PPY92_000346"
     CDS             complement(24296..24601)
                     /gene="icaD"
                     /locus_tag="PPY92_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular adhesion protein D"
                     /protein_id="HDJ2624372.1"
                     /translation="MVKPRQREYPTLKSSLNIVRETALIAISCVFWIYCLVVLLVYIG
                     TIFEIHDESINTIRVALNIENTEILDIFETMGIFAIIIFVFFTISILIQKWQRGRES"
     gene            complement(24565..25803)
                     /gene="pgaC"
                     /locus_tag="PPY92_000347"
     CDS             complement(24565..25803)
                     /gene="pgaC"
                     /locus_tag="PPY92_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HDJ2624373.1"
                     /translation="MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVD
                     ELEGITFLLACYNESETIEDTLSNVLALKYEKKEIIIINDGSSDNTAELIYKIKENND
                     FIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAV
                     TGNPRIRNKSSILGKIQTIEYASLIGCIKRSQTLAGAVNTISGVFTLFKKSAVVDVGY
                     WDTDMITEDIAVSWKLHLRGYRIKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLL
                     RDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGYLFITANFLDYTFMTYSFSIFL
                     LSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVYWIINAAVVLVAFPKAL
                     KRKKGGYATWSSPDRGNTQR"
     gene            25967..26527
                     /gene="icaR"
                     /locus_tag="PPY92_000348"
     CDS             25967..26527
                     /gene="icaR"
                     /locus_tag="PPY92_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ica operon transcriptional regulator IcaR"
                     /protein_id="HDJ2624374.1"
                     /translation="MKDKIIDNAITLFSEKGYDGTTLDDIAKSVNIKKASLYYHFDSK
                     KSIYEQSVKCCFDYLNNIIMMNQNKSNYSIDALYQFLFEFIFDIEERYIRMYVQLSNT
                     PEEFSGNIYGQIQDLNQSLSKEIAKFYDESKIKMTKEDFQNLILLFLESWYLKASFSQ
                     KFGAVEESKSQFKDEVYSLLNIFLKK"
BASE COUNT         9485 a         3963 c         4768 g         8424 t
ORIGIN      
        1 aataaaaaac ccgttaacgt gttgttaacg gttaataagc acatatttat tttaaatttt
       61 ttaatatacg agacatctct tcaattttac cagcatgaag tgggatgtga atggcattta
      121 atactaataa ctcttctaag ttattacaac caacaattgg ttgtttcaat tcaattgcta
      181 aatcatcttc agttagattt cgtgcacgtt caggtaatgt ttggagacct tctaaaatct
      241 tttcaatact cggtacttca tccttccatt catctggaga cgaaccattt ccgaataaag
      301 gtctatagat atttaaatca atattctctt taccagcaac agctaaggcc gattcaaaaa
      361 ttgttaatac atgcccaaat tgccaatgaa tggtattaga aaaaggctca tgataatcat
      421 caagtacctt ttcaactttc caagcatcat acacatttac taaatatcct aaccccatat
      481 caatgacatc aaatactgtt tttgtagtca ttattaattc ctcccccata aataaaacat
      541 acttccgtaa gtattaccct attaacaaag ttgttagtca tttaaggaat gtgtacaaca
      601 actaccttac aataaaagat agttgacgaa aagttctcaa tgtactatct tattatgtaa
      661 gatagtaggt gattaaaatg tcagtgtcag atcagattat gaaaggtctc ttagatgggg
      721 cgatattagg tctcattgcc caaggcgaaa cgtatggata tgaaattatg acgaaactta
      781 agaatcaaga gtttccagag ataagtgagg gtagtattta tccagtttta atgcgtttaa
      841 acaaaaaagg atacgttgat acgacaataa aaaaatctag taatggcggc ccgaggcgga
      901 aatactatac gattaccgat tcaggtttaa aggaactaaa agtatttaaa tcaaaatggc
      961 aagctttgaa taaagggatg attaacttgt ttggagggga aaacgatgtt aactaaacaa
     1021 tctcaagatt ttttagaaaa attacgtgtt gaattgttat ttagaggtaa gactgaagca
     1081 gaagtagaag agttattgga agagctcgaa gatcatttaa ctatggctga aaaaaataac
     1141 gaagatacaa gttcaattat taatacaccg attaagtcaa tggctgatca attatcacct
     1201 gagataagtc ttactaccgg tttgtataaa tatattactt tgtacttctc ttttatatta
     1261 gcagtcatta taattcctag gtttttagat ttgggtacat ttgatgtgac aattgcattt
     1321 ttactttata tagctagtat cgttatttta ggtgttgtca ttggtatgct gattctacgc
     1381 aatacattag tccgatttgg tgaccaaaaa ataacgtatg ttataaatgt gacttatgga
     1441 attgtcgtat ttttatggat gatttttggt ggtttgatta ttaaaaaata tcctatctat
     1501 agtttctttg agttaagtga caaacagagt ttcatcgttg ggttagtact tttaattgcg
     1561 cttattgtaa tttgtatttt aattaaacag aaaatttatg cgctcatcgt gttggtaatt
     1621 tcattgccaa gtttgatagc aaggatagca gctatcttta gtaaagaacc agacactttt
     1681 aatttagttt cagtaagtat actcatcatt ctaaatatag catttattat ttatacattt
     1741 gtaaatttta ggaagttaaa aaaagaagac taaacaatgt cgtttagcta aaatacatca
     1801 ttatagtaaa actgtatacc aatcatccaa aagcacccta aagttagact taaactaaca
     1861 aattagggtg cttttgtttg tgcatttata actattccgt tgtgcctaga gctatacgta
     1921 aatcctcatc attagaaata gcttcaatgg ctgcagtcaa acctgcaact atcttttcta
     1981 atggcataga tggtgtatcg ggtttaccaa tgacctgctc tggtatgtat ggcacatgaa
     2041 taaatccgaa tcgtaggtga gggtaatgct tatcttgtaa ataacctaag tgataaagca
     2101 cgtgattaca tacatacgta cctgcgctat ttgaaagtgc tccaggaagt ccttgattaa
     2161 taatactttg agtcattgct ttaactggta aatttgaaaa ataagctggc gcaccgtcta
     2221 agtgaatggc ttgatcaata ggttgaaaat catcattatc tggaatacgt gcatcatcaa
     2281 tattaatggc gacacgttct ggggtaatgg catttctacc accagcttgt cctatagcta
     2341 gtacaacatc ataatgatta gatgccaacg ttttatttat aatattatct actttcttaa
     2401 aagaggttgg tagttttaat ttatcgattg tatgtgtgcc aataatatct tctagttgag
     2461 tcacagcttc ccatgaggga ttgatatttt gattgtcaaa aggcgcaaac cctgttacta
     2521 aaatgtgcat tttaagacac cccgcagtat taaaatttaa tttagtataa cacgcgcata
     2581 attaaaaaca tatcaggtta tgtatgcgca taaaaaacag ccttgaaaaa cctttataaa
     2641 tcggattttc aaagcgtgtt ttgaaaggta tttaattaaa actaagcatg ttgatgtaaa
     2701 tcatcaaagt ttgttaaacg ttgttgccac tcattatcac taatattatt tgcttgaaca
     2761 taacgattac gctcattcat aagagcatgc acctaaatat ttagtttcgt tttcttcaga
     2821 aactaatatt tgtttattac attctgggtt agcgcaatta atataacgtt cacatggttt
     2881 gccatcaaac caatccttac caataattgt tttttcaact tggttgatat caacactgat
     2941 acgatcatca aatacataca ttttaccgtc ccaatattga ccttttgttt cagggtcttt
     3001 accatatgta gctataccgc cgtgaagctg cgctacatct tcgaaacctt cttttaaaag
     3061 ccatccagaa aatttttcac atcgaatgcc acctgtacag tacgtaacca cttttttatc
     3121 tgtaaataac gctttattct ctttaatcca gtcaggtaaa tctctaaagc gtgtgatgtc
     3181 tgggcgaatt gcacctcgga aatgacctaa atcaaattca taatcattac gtgcatcaat
     3241 aatgactgtg tcatcatctt caagagcttt tctaaattct acaggtgata aatattggcc
     3301 agttgtgtgt cttggatcga cgtcatcttc taaatctaaa gcaacgattt cttttcgagg
     3361 acgtacatgc attttcttaa aagcatgtcc ttcagcttca tcaattttaa acaccatatc
     3421 tttgaatcgt tcatcggcat gcatatgtgc catatattgt tcggtttctt cttttgtacc
     3481 agataatgtg ccgttaatac cttctgtaga aacaagaatt ctacctttta aatggtgtgc
     3541 tttgcaaaac gctaagtgat cctgagcaaa ctgttcaggg tcatcaatcg tcgtatattt
     3601 ataatataaa agaacttgat agttcatagg ttaatctcct tctataaaca tgttataagc
     3661 atgttaattt ttaaaataaa gcgtcaatac gcatatttat ctacgtccat cattttaaca
     3721 gagttgtatt gtcattcaat atttctagct tgaaatcagc cactagaccg ataaaataga
     3781 tagatgaaac cgttatcatt ttatttgata gttatcttgt ttaaaataaa attgatagtt
     3841 aaaacgaatg aaatgaaagg aatgatcatg atgtcacaac aagatttacc tacattattt
     3901 tatagcggga agtccaatag tgctgttcca attatatctg aaagtgaatt acaaacaatt
     3961 acagctgaac catggcttga aatttccaaa aaaggattgc aactagaagg attgaacttt
     4021 gatcggcagg gacaactctt tttattggat gtattcgaag gcaatatttt caaaatcaat
     4081 cctgaaacga aggaaatcaa acgatctttt gtaagtcaca aagcgaatcc tgcagcaatc
     4141 aaaatacata aagatggccg attattcgtt tgttatttag gagattttaa atctacagga
     4201 ggcatttttg cagctacaga aaatggtgac aacttacaag atattattga agatctttca
     4261 acagcatatt gtattgatga catggtattt gattctaaag gtggatttta ttttacagat
     4321 tttagaggat actctaccaa tccactagga ggcgtttatt atgtttcgcc ggactttaga
     4381 acagtgacgc ctatcattca aaatattagc gtagcaaatg gtattgcttt aagtacagat
     4441 gaaaaagtac tatgggtaac agaaactaca gccaatcgat tacatcgcat tgcacttgaa
     4501 gatgatggtg tgacgataca accatttgga gctactatac cgtactattt tacaggtcat
     4561 gaaggaccag actcatgttg tattgatagt gacgataatt tatacgtagc aatgtatggt
     4621 caaggtcgag tgttagtttt taataaaagg ggttatccaa taggacaaat attgatacca
     4681 ggccgagatg aagggcatat gttacgttct actcatccgc aatttatacc tggaacaaat
     4741 caactcatca tttgttccaa tgatatagaa atgggcggag gatctatgct ttatacagtt
     4801 aatggctttg caaaaggtca tcaaagtttt cagtttcaat aagacctacc tgaatgaaag
     4861 gcgtcttaaa ttagggtgaa cagttagtgc ttgttacaca tgagttaaac gaaaaatgcc
     4921 aactatacct agggatgata tagttggcat ttcgtatata tatattaaaa caatgaggat
     4981 tgtgtaatga gattattctg agattgagaa ttcagctgat gcttcgaatt taacatcttt
     5041 gcctagcatt acgccaccag tttcaagtgc ttggttaaag ttaatgccgt agttttctct
     5101 attgattgta ccagtaacaa taacacctgt tacttgagaa ccatccatag gattcttact
     5161 tactccgttg aattcaacgt cgaatgtttc ttcgttagtg atacctttaa ttgttaaatc
     5221 accaacaact ttgttttcag taactgattt tgtttcaaac gtgattttat caaattcatc
     5281 agtaccaaag aaatcaccag attttaagtg gttatcacgt gcttcgtttt tagtgttaat
     5341 tgagcttgga ataattgttg cagtagcttt caaagtactg aagtcattaa tatctccttc
     5401 aacagctaca tcaaattgat caaatgaacc tttcacttta gaaaccatta aatgtttaat
     5461 ttggaattct aaactactgt gtgcaccatc aaaagtaaag ttagtcataa tataaaacct
     5521 ccatattttt atctcttaga tatatttttt atacttgata tacatacaat accacctaaa
     5581 aaacaaagat acaagcattt tatttcgaat tcgaatattt tctgtgatgt atttaaagaa
     5641 tgacctataa aacaccacac tattgttata ccctagtcaa ttaattcaaa cataaagaag
     5701 tgattacaag tccgtgaaat ttagttaatg agataaatct gactattatt atcattttta
     5761 tagtcactac tattgacttt gcatcaagtg actcgtacta ttttaatata attctgaaaa
     5821 ttcaaaatat agaacgttaa atatgtagat tatatttttt tgaagtttta aaaaaggagg
     5881 cattttatgg gaaattattt ggttttgcaa tcagattttg gacttagtga tggcgctgta
     5941 agtgcaatgt atggagttgc atatactgta agtgatgata tacgtgtcga aaacttaacg
     6001 catgatatac caccgtatga catttgggta gcgtcatatc gtttatatca aactgtaaaa
     6061 tactggccta aaggcacagt ttttgtatca gtggtagacc cgggtgtagg tagtgatagg
     6121 cggagcattg cttgcttaac atattcaggt cattacatta ttacacctga taatggttct
     6181 ttaagtcata ttaaacatta cgaaggtatt aaaaaagtta tcgaaattga tgaagtgaaa
     6241 agccgattgc ctcattcgga agaaagtcat acttttcatg gtagagatgt ttatgcatac
     6301 aatggtgcga gattagctgc aaataagata gcgtttgaac gtctcggtca ggcaattaat
     6361 gttgatagta ttgaggcatt agaaataaga gaagcgacaa aaaatgagga ttctgttaca
     6421 ggaagcattg atattttaga tatcagattt gggtaaatat tccattggca ttcttaaaat
     6481 ccttggaaat tgttcatggt aataatttgg tggtaacaat ttatcatcaa gataagaaag
     6541 tctatcaaaa tattattaaa tttgcgcgat cgtttgcaga tgttaatgtg ggtgaacctc
     6601 ttgtgtatgt taactcgcta gtaaatattg gtgtagcagt caatcaagac tcattttcag
     6661 atttatatca tattggtact ggaaatgatt ggacgattca tttatctaaa gcaccatcaa
     6721 tttaatgaat tttttatgta aggagtaatg aagatgaaaa aacaagatat ttcagttaaa
     6781 actgttgttg cgattggtat aggagcagcg gtatttgtga ttttagggcg ttttgttgta
     6841 ataccaacag gttttcctaa tacgaatata gaaacatctt atgcattttt agcattaata
     6901 tctgcaatat ttgggccttt tgctggttta atgactggat tagttggaca cgctattaaa
     6961 gatttcacga catacggtag tgcttggtgg agttgggtta tttgttcagg aattataggc
     7021 tgtttatatg gatggattgg cctaaaatta aatctttctt caggcctatt ttctaggaaa
     7081 tcgatgattt attttaatat cggtcaaatt attgcgaata ttatttgctg ggcacttatt
     7141 gcaccaacat tagatatttt gatttataac gaaccggcta acaaggttta tacacaaggt
     7201 gttatctctg cagtattaaa tattatttca gttggtatta ttgggacaat attattaaaa
     7261 gcatatgctt catctcaaat aaaaaaaggt agtttacgta aagaataatc attttgttga
     7321 atcagatatg taaatgaatg tagaaaggta atgatatatc atgactgaac caattatctc
     7381 gttcaaagac tttagttttc aatatcatag tcaagcaaca cctacattac agaatataaa
     7441 tgttgatatt tatccaggag aaaaagtgct agtagttggt gcttcaggta gtggtaaatc
     7501 gacttttgca aattgcataa acggattaat tccatttaaa actaaaggta acataaccgg
     7561 ggaactatat ataaataatc aagatgcaac cgttagttgt ttacatgata gatctaatgt
     7621 tgttggtaca gttttacaag atacagatgg acagttcata ggcttaacag cagctgaaga
     7681 tatggccttt ttattagaaa ataattgtgt tgaacaagat gatatgaaga aaaatgtaag
     7741 ttattgggct gaaaaagttg gcatgataga acatttaaat caccgaccgc aagatttatc
     7801 tggaggtcaa aaacaacgcg tttcattagg tggtatatta atccatcgta cgcctatttt
     7861 aatattggat gagccgctgg ccaatttaga tcctgcgaca ggacatgaaa cgctgagatt
     7921 gttaaacaat attcatgaag aaacaaagtc aacgatgatt attgtcgaac atcgattaga
     7981 agaatcatta gatgatacgt ttgatagagt tcttcttttt aaagatggaa aaatcatcgc
     8041 taatacaacg cctagtgatt tgctaaaatc atctaagctt aaagaagcag gcatacgtga
     8101 accattatac tgtacagcgc ttaaatatgc agaagtagat gtcgaatcca ttgataactt
     8161 agcgaattta cgtgacgttt gtatgagtga acatgttaaa tttaaagtga aaaaatggat
     8221 agatgaaaca tctgcaaata atgataataa gtacaaatct gaaccgctat tagaattaaa
     8281 tgaggtatgt gttcaatata gtgactatag taatagtgta cttaataatg ttcaattaaa
     8341 tgtttataga agagaaatgc ttagtatagt tggtcacaat ggtgccggca aatcaacact
     8401 tgctaaagca atttgtggat ttttagatat aacaggtaat atccaatttt gtaatagggg
     8461 atttaatcaa ttgtcaatta gcgaacgatc tgaattcgta ggttatgtga tgcaaaatcc
     8521 aaatcatatg atttctgaaa aaatgattta cgatgaagta gcattagggt taagagcacg
     8581 aggtatgaaa gaatcagata taaagatacg agtagagaat gtgctcaaaa tatgcggact
     8641 ttatgcattt cggaattggc caattgcagc tttaagctat ggtcaaaaaa agagggtcac
     8701 tatagcatct gttttagtct taaatccgga aataatcata ttggatgaac cgactgctgg
     8761 tcaagatttc tatcattata atgagataat gtcattttta attgagctaa acagacaggg
     8821 aaagacgatt attatgatta cgcatgatat gcatttattg tctgagtata gttcaagaac
     8881 agttgtatta tcaaaaggac aagtcgttgc tgataccacg ccagtattga tattaaatga
     8941 taaaaaaatc tgtgagattg catcattgag acaaacatcg ctatttgaaa tggccgaata
     9001 tatagggatt agcgagccac agaaattagt acaattattt attaaccatg ataggaaggt
     9061 gagacgccaa tgaatcaata taatactata ggttttcacc cgggaaatag tcgtattcat
     9121 caattaaatg cgactgttaa acttttattc ttattagttg tttctatttc tgcaatggtg
     9181 acttatgaca caagatattt aattttaatt agtgcttcat ctattttatt gttcaaatat
     9241 gctcatattg aatggaaaca agttcgattt gttgttaaat tcattctgtt tttcacaata
     9301 ttaaatatta ttgccgtata catatttgac cctgaatatg gtgtgaagat ttataatcag
     9361 cgtacagagt tagtcaatgg tattggtcga tttacgctaa catcacagga attattctat
     9421 ctttttaatc taatattaaa atatattagt acagttcctt tagcgttaat atttttattc
     9481 acaacgaatc cgagtcattt tgctgcaagt ttaaatcagc taggtgtgaa ttataaaatc
     9541 agttacgcag tctcactagc attaaggtat attccagata ttcaagaaac atattttaat
     9601 atttcacaag cgcaacaagc aagaggatat gataattcga aaaaagcgaa atttactagc
     9661 cgtgttaaag gtatcaaacg cattgtgtta cctttaatat tttctagtat cgaaagaatt
     9721 gacactatta gtactgctat ggagttaaga caattcggac agtataaaag gagaacctgg
     9781 tacgtcaaaa aacaattaaa aaaagatgat tatgttgttt tgtgtttgac gttaatactt
     9841 ctgatgttag tagttacatt attcttttta aataatagtc gatatttcaa cccgtggcat
     9901 tagtattcat ataaatagtc tttaaataga aataggaggg agacatttaa tgataaatac
     9961 tgaaagatta aatttaatga ttccaagttc ctcgcattta attgaacttt ataatatttg
    10021 tagtcatcca caagcaaata tatacactcc caaaggttta cataattcca aattagacac
    10081 acaacggtgg attgaaaaat ggcaaaacca ttggcaacaa tatcaatttg gttactttgt
    10141 attggtgaaa aaaatagatt gtagtgttat tggtatttgt ggatatgaat atcgacaatt
    10201 aaagcaagaa acagtactaa atttatttta taaattacat ccaagttttg aaggacaagg
    10261 gtacgcatgt gaggctatta cagcaatcac aaattttgtg aattatatcg atcaagaaac
    10321 agtaaaagtt atcaggacaa ataagtgtaa ccaacgttca ataaatttag cagaaaggct
    10381 taaattcaag cgagacaaag ctatggacga cattatcaat caaggagata ttgtgtttta
    10441 taaataaaat actatgacat tatctaaaaa ataaaattaa aatcccaaca gtttcttaaa
    10501 aagttatgag ccgcataata aaaatgttat aattgtgcat aataaaacat agggagaatg
    10561 tttaatgcat aaaaaatatt tattgctatt ttttctgtta ttatcagttg cattgctata
    10621 tagctgttcc aagcaaaatg agaaagctca aaataaatct gttaatccaa atgagtatgc
    10681 aaagcataaa tcgaaaatgg atgaacattg ggaaacatat cacggccaag tttatcatgt
    10741 gttttatcac ccattaatta cagacccaaa agttgcgttt acaggagata cacaccaagc
    10801 taagggcaac aatgattgga tgattactgt aagtgaattt aaaaaatcat tgaacgaatt
    10861 atataaaaat aattacatca tcgttaatcc acatgatgta tacgacttat catctcatca
    10921 tgtaaaagcc aaaaaaataa aattgccgaa aggtaaaaag ccactaatca tctcaattga
    10981 tgatatgaat tactatagtt acatgagaca ccatggttat gctgaccgtt tagtactcga
    11041 taaaaataaa catgttgttt ctgaaactac agacaaaaaa ggtcatgcta cttattctga
    11101 tgacaatgat atcgtgccaa ttttaaataa atttgtaaaa gagcatccag atttttcttt
    11161 aaatggtgaa aaaggtgttg ttgctttaac cggttatgag ggcgtattag gttatcgcac
    11221 gaatgaatta acgagtaaag attatacaaa gaataaaaag gcagctgagg aagttgttcg
    11281 cgcaatgaaa cgggatggat ggtcatttgc gagtcattca tatggtcaca ttaattttga
    11341 aaaaacatct ttagaaggta ttaaacgtga tactaaaaga tggaaagatg aagttgaacc
    11401 aattgtaggt aaaacagata tgtttgtatt tccacatggc gcacaggata gacatacaca
    11461 agcgtatgat tatttggtag atgaagcaga gtttaagttt attgcaggtg tcggtccaaa
    11521 caattttaca gatatatcag caacgaacgt ttaccaagat agggttgcga ttgatggtct
    11581 aaacttattt gaatttaaat ataaactcaa accgtttttt aatcctgaaa atgtatatag
    11641 taaacaagat agacaattct ttaaaggtaa tcgggattat gaagaatagt taaatttaat
    11701 aaaaagatag aaagaaaata actttacata ataaggcaag cacaatatgt gcaagaagta
    11761 caacttcaat ataaaaatct tggtttagca tttttatagg cacaaatgtc ttgccttttt
    11821 cttttgtaaa aatttaaaaa caatacaccg aattttctga aaaaggttgt aatgtattgc
    11881 taaaaatgat aagttattaa tcatattaca ctttagtgtg gtagagaagt aaagtaaaca
    11941 acgaaaatac caagtgattt taaatacagt attgaaagta aagggtggtc gagtgaataa
    12001 ttcagaacaa ttaattgcgt taaaagaatc ggaaacagca tttttaaaat attttaataa
    12061 agcggattat gagttagttg attttagtgt ggttgaaaag ttggactgga aacaattgaa
    12121 ccatgaggat ttgcagcaga tgggtgagcg aaatttttgg caacatgagc atcaaattta
    12181 tgcacttcga aatgatttta cagatcaatt attgcgttat tactcaatgt atccaactgc
    12241 agcaacaaaa gtggcataca caggtttaat cattcggaat aatgaagcgg ctgtacaagt
    12301 gggtttggaa aactatgcac catcattagc gaatgtacaa cagagcttaa agctatttat
    12361 tcagtttatt caacagcagt tgagagacaa cgtgcatttt gttgtattgg gacattatca
    12421 attgcttgat gccttactcg ataaatcgtt acaaacacca gacatactat caatgattga
    12481 ggaacgtaat ttatcgggac tggttacata tttgtcgact gaacatccaa tcgtgcaaat
    12541 cttgaaagag aatacgcaac aacaattaaa tgtattggaa cactatattc caaatgacca
    12601 tccagcactc gttgaattaa aaatatggga acgttggtta catacacaag gttacaaaga
    12661 catccattta gatattactg cgcagccacc tagatcctat tacacaggct tatttattca
    12721 atgtcatttt gctgaaaatg aatctcgagt tttaacaggt ggttattaca aaggaagcat
    12781 cgaagggttt ggattaggat taacacttta agtaagggag tatgcacaat gttaagaatc
    12841 gccatagcca aaggacgtct aatggatagt ttaattaact atttagatgt aattgaatat
    12901 acgacattat cagaaacatt aaaaaataga gaacgccaat tattattaag tgtagataat
    12961 attgaatgca ttttagtaaa aggaagtgac gtgccaatct atgtggaaca aggaatggcc
    13021 gacataggca ttgttggtag cgacatatta gatgagcgcc aatataatgt taataatttg
    13081 ttgaatatgc cttttggagc atgtcatttt gcggttgcag cgaaacctga aacgaccaat
    13141 tatcgtaaaa tcgcaacgag ttatgttcat actgctgaaa catattttaa atcaaaaggt
    13201 attgatgtcg aattgattaa attgaatggt tctgttgaat tagcttgtgt cgtagatatg
    13261 gtagacggaa ttgtcgacat cgttcaaaca ggtactacgc taaaagcgaa cggactggtt
    13321 gaaaagcaac atattagtga tatcaatgca agattaataa ctaataaagc agcttatttt
    13381 aaaaaatcac aattaataga gcaatttatt cgctctttgg aggtgtctat tgccaatgct
    13441 taatgcacaa caatttttaa atcaattttc attagaagca ccattagatg agtcattgta
    13501 tccaattatt cgcgatattt gtcaggaagt taaagttcat ggagataagg ctttgaaaat
    13561 gtataatcta acattcgatc atacgaaaac agatcattta gaaattagtc atgaacaaat
    13621 taaagcagca tttgacacat tagatgaaaa aacaaaacaa gcattacaac aaagttatga
    13681 aagaattaaa gcatatcaag aaagtattaa gcagacgaat caacagttag aagaatcagt
    13741 ggagtgttat gaaatatacc atccactaga aagtgtgggt atttatgtgc ctggtggcaa
    13801 agcaagttat ccatcaacgg ttctaatgac agcgacttta gcacaagtag cgggtgtaga
    13861 aaatattgtt gttgtgacac cacctcaacc taacggagta tcccaagagg tattagctgc
    13921 atgttatatt acgcaagtta atcaagtgtt tcaagttggt ggtgctcaaa gtattgctgc
    13981 attgacttat ggaacagaaa cgatacctaa agttgataag attgtaggtc caggtaacca
    14041 atttgttgca tatgccaaaa aatatttatt tggacaggta ggtattgatc aaattgcagg
    14101 accaacagaa atagcactga ttattgacga caccgcagat ttagatgcca tcgtatatga
    14161 tgtatttgcg caagcagaac atgatgaatt agcacgtaca tatgtcattg gtgaagatgc
    14221 gcaagtcctt aaagatttag aatcacgtat tgctaaagca ttgcctaatg tggacagata
    14281 cgacattgtt tctaaaagta tcgctaatca acactacctt atccatgcta gtaattttga
    14341 tgaagcatgc catgtcatga atacaatcgc gcctgaacat gcgtcgattc aaacagtaaa
    14401 tcctcaacca tatattgaaa aagtgaaata tgtgggtgca ttgtttattg gacattattc
    14461 gccagaggtc ataggagatt acgttgcagg tccaagtcat gtattaccta caaatagaac
    14521 agctagattt accaatgggt tatcggtcaa tgatttctta acacggaaca cggtcatcca
    14581 tttatcaaaa gatacgtttg aacaaattgc tgattcagca caacatattg ctcatgttga
    14641 agcattatac aatcaccagc agtctatttt aatacgtcag tcttagggga gtgtaattga
    14701 aatgatttat attgataaaa atgaaagtcc agttacgccg ttggatgaaa aaacaatgac
    14761 gtctattatt agtgcaacgc catataattt atatcctgat gcagcatatg aacaattcaa
    14821 ggaagcttat gctaagtttt acggattatc gcctgaacaa attattgcag gaaatggatc
    14881 tgatgaattg attcaaaagt taatgctgat catgccagaa ggtccggcat taacgctaaa
    14941 tcctgatttt tttatgtatc aagcatatgc ggcacaagta aatcgtgaaa ttgcatttgt
    15001 agatgcagga tcagatttaa cgtttgattt ggaaaccatt ttaacgaaaa tcgatgaagt
    15061 acaaccatca ttttttatta tgagtaatcc acataaccct tcaggcaagc aatttgatac
    15121 ggcattttta acagctattg cagataagat gaaagcatta aacggatact ttgtcattga
    15181 tgaagcatat ttagattatg gtacggcata tgacgtggaa ctggcaccac acatcttaag
    15241 aatgcgtaca ttatcaaagg cgtttggaat tgccggctta agattaggtg tcttaattag
    15301 tactgctgga acgataaagc atattcaaaa aatagaacat ccatatccat taaatgtatt
    15361 tacgctaaat attgcgactt atatttttag acatagagaa gagacaagag tatttttaac
    15421 gatgcaacga cagttagctg agcagttaaa acaaatattt gatacacatg ttgcagataa
    15481 aatgtcagta ttcccatcaa atgccaattt tgtacttact aaaggttcag cagcgcacca
    15541 attaggacaa tacgtatatg aacaaggatt taaacctcgc ttttatgatg agccggtgat
    15601 gaagggctat gtaagatact caattgcaac agcatcgcag ttaaagcaat tagaagaaat
    15661 tgttaaagaa tggagcgcaa aatatgattt atcaaaaaca acgaaacaca gctgaaacac
    15721 aactaaatat ttcaatatca gatgaccagt caccatcgca tattaataca ggtgtgggct
    15781 ttttaaatca tatgttaaca ttgtttacat ttcatagcgg tctgtcatta aacattgagg
    15841 cacaaggtga tattgacgta gatgatcacc atgtaactga agatatcggc attgtcattg
    15901 gccaattgtt acttgaaatg attaaagata aaaagcattt cgttcgttat ggaacgatgt
    15961 acattccaat ggatgaaaca ttagcacgtg tcgttgtgga tataagtggg cgcccatacc
    16021 tatcattcaa tgcatcatta agtaaagaaa aagttggtac gtttgatacg gagttagtag
    16081 aagaattttt tagagcggtc gtaatcaatg caagattaac aacgcatatt gatttaattc
    16141 gtggaggtaa tacacaccat gaaattgaag ctatattcaa agcgttttcc cgtgcattag
    16201 gcatagcgct aactgcaact gatgatcagc gtgtgccgtc atcgaaaggt gtgattgaat
    16261 gattgtcatc gttgattatg gattagggaa tattagtaat gtaaaacgcg ctattgaaca
    16321 tttagggtat gaggtggttg tctcaaatac ctcaaaaata atcgatcaag cagaaacaat
    16381 catattgccc ggtgtcggcc attttaaaga tgcgatgtca gagataaaac gattaaatct
    16441 caatgcaata ttggctaaga atactgataa gaagatgatt ggtatttgtt taggcatgca
    16501 attaatgtat gagcatagtg atgaaggcga tgcatctgga ttagggttta tcccaggaaa
    16561 tatttcgcgt atccaaacag aatacccagt gccgcactta ggttggaata atttagtgag
    16621 taagcatcct atgttaaatc aagatgttta cttcgtacat tcttaccaag cgccgatgtc
    16681 agaaaatgtc attgcatatg cgcagtatgg ggctgatatt ccggcaattg ttcaatttaa
    16741 caattatatt ggtattcaat tccatcctga aaaaagcggt acatatgggt tacaaatttt
    16801 gcgtcaggca atacaagggg gatttataaa tgattgaatt atggccagcg attgatttga
    16861 ttgggtcaac aagtgtgagg ttaacagagg gtaaatatga tagtgaagaa aaaatgtcgc
    16921 gttcggctga agaaagtatt gcttactata gtcaatttga atgtgtgaat cgtattcata
    16981 tcgtcgactt gataggtgct aaggcacagt atgcccgaga gtttgattat attaagtcat
    17041 tgaggagatt aacaaccaaa gatattgaag taggtggtgg cattcgtacg aagtcacaaa
    17101 tcatggacta ctttgccgca gggattaatt attgcatagt tggaacgaaa ggcattcaag
    17161 atactgattg gcttaaagag atggcacata catttccagg tcgtatttat ctgtcagttg
    17221 atgcttatgg agaagatatt aaagtgaacg gatgggaaga ggacacagag ttaaatttat
    17281 ttagttttgt gagacagtta tcggatatac ctcttggagg cattatatat actgatattg
    17341 ctaaagatgg caaaatgtcc ggacctaact ttgaattaac tggtcaatta gtaaaggcaa
    17401 cgacgattcc tgtcattgct tccggtggta ttagacatca gcaagatatt caacgattag
    17461 cgtcgctaaa tgttcacgct gctattatag gaaaggctgc acatcaagca tctttttggg
    17521 aggggctaga atgattaaaa aacgtatcat tccatgttta gatgtcaaag atggtcgtgt
    17581 cgttaaaggg attcaattta aaggattaag ggatataggg aatcctgttg atttagcaat
    17641 atattacaat gaagcgggtg ctgatgaatt agtattttta gacatctcta agacggaaga
    17701 gggtcatagc ttaatgctag aagtgattga acagacagcg tcacgcttgt ttatccctct
    17761 tactgtaggg ggtgggattc aaagtctcga tgatattacc caattgttaa atcatggtgc
    17821 agataaagta tcattaaatt caagtgcttt aaaaaatcca cagctcatta aacaagcgag
    17881 tgataaattc ggtagacaat gcatctgtat agcaattgat agctattatg atcctgaaag
    17941 aaaagcacat tattgttgta cgcatggtgg taaaaaaatg acaaatatta aagtatatga
    18001 ctgggtacag caagtagaac agttaggtgc aggtgagctc ctcgttacaa gtatgggaca
    18061 tgatggtatg aaacaaggat ttgatattga gcatcttgca aaaataaaat cccttgtaaa
    18121 tattccaatc attgcttctg gtggtggtgg caatgcacaa cactttgtag aactatttaa
    18181 tcagacggat gtttctgcag gtttagctgc aagtatatta catgaccgag aaacgacggt
    18241 tcaatcaatt aaagaagtga tacggcaagg gggtatagca gtaagatgac caaatataaa
    18301 attgatttta gcaaaggttt agtgccagca attttacaag ataatcaaac aaaacaagta
    18361 ttgatgttgg gttatatgaa tcaagaagct tttgataaaa cgatagaaga tggtgtggta
    18421 tgtttctatt cgcggtcgaa acaacgtcta tggacaaaag gtgaaacatc tggtcatacg
    18481 caacttgtta aagatattca tgtagattgc gacaatgata ctattttaat tgatgtcata
    18541 ccaaatggac caacatgtca tacaggcagt caaagttgtt tcaacacaga agttccattt
    18601 tcagtgcaaa cattagcgca gacagttcaa gatagtgccc aatccaataa tgaaaagtca
    18661 tatacaaaat atttattaac agaaggtata gaaaagatta caaaaaaata cggtgaagaa
    18721 gcttttgaag tcgtaattga agcaattaaa ggtgacaaaa aagcatttgt aagtgaagta
    18781 gcagatgaac tttatcattt atttgtcttg atgcatgcgc taggcgtcga tttttcagaa
    18841 attgaggcgg aattagcgcg tagacatcat aagcgcaata actttaaagg tgaacgacaa
    18901 aatatcgaac agtggtaaag caagtatgga ctaagatata aggaaaagga tcatggctta
    18961 tacacttaca aatattgtgg aaaacgtgac attttcaagt ttaaaatacg acaccaacat
    19021 attttaacta tgaatgctgt gatggtacta aagttgcgaa ctcgttatag ataagtagtg
    19081 gataatcaca atacgaaatc aaaaataatt atgaaaagta aattgagcaa ctcaggaata
    19141 gatgtcactg ttaaagatgt cgaaaagtat atgaatcgat ataatgaagt tatgatggga
    19201 aaaaatggcg aaaaagctaa agagttatgt ttgtcgttac tacctattaa tatcatagtt
    19261 gtctttacat tctttgtatt tatactataa atacaaatat atctagcctg aaatagaaat
    19321 gtcatagcct atttaaaaga caatctccat tagaactaag atgtgcatcc cgaaagttag
    19381 actaaaaaac taactttatg gggtgtattt ttatgctaat catcataaat tcgagattaa
    19441 gttaaaggta gttcaagagt aattaaacaa taaattaaaa atagtaggat acttactttg
    19501 agggaagaaa attaactgta tatatttaat gtaggaacaa gtattacggt ttatcctgat
    19561 acaatcattg tggatgggat gatattttta ggtttaaaat acgacaccag caaacataat
    19621 aactgtaata gctcataaat ctctccatac agctaatcta aaaaaataat acatcattgg
    19681 aatcaagacc caagcatgta aatattaaaa atcacaaaag atatatgtaa aaaagttaca
    19741 atttgcataa ttaaattgtg tctaattatt gactaattaa attttgccaa atataatatt
    19801 aattaataat ttgaaatgat tagcgtatac actttaaatt ctctttggag aatatatttt
    19861 ttaaatacaa atgtaaacgc tttctcgtca aattaaacaa tagaaaggat ggtcattatg
    19921 agtgcttggt taagtaaatt atttgagttt attcctcgaa taattatcaa tttgtttatc
    19981 taaactaaaa aaatagaggt gctgacaatg atgaaaagtc aaaataagta tagtattcgt
    20041 aaatttagtg taggtgcatc ttccatttta atagctacat tactattttt aagtggtgga
    20101 caagcacaag cagctgagaa gcaagtgaat atgggaaatt cacaggagga tacagttaca
    20161 gcacaatcta ttggggatca acaaactagg gaaaatgcta attatcaacg tgaaaacggt
    20221 gttgacgaac agcaacatac tgaaaattta actaagaact tgcataatga taaaacaata
    20281 tcagaagaaa atcatcgtaa aacagatgat ttgaataaag atcaactaaa ggatgataaa
    20341 aattcatcgc ttaataataa aaatattcaa cgtgatacaa caaaaaataa caatgctaat
    20401 cctagcgatg taaatcaagg gttagaacag gctattaatg atggcaaaca aagtaaagtg
    20461 gcgtcacagc aacagtcaaa agaggtagat aatagtcaag attcaaacgc taataacaat
    20521 ctaccttcac aaagtttaac aaaagaagca ccatcattaa ataagtcaga tcaaacaagt
    20581 caacgagaaa ttgttaatga gacagaaata gagaaagtac aaccacaaca aaataatcaa
    20641 gcgaatgata aaattactaa ccacaatttt aacaatgaac aagaagtgaa acctcaaaaa
    20701 gacgaaaaaa cactatcagt ttcagattta aaaaacaatc aaaaatcacc agtagaacca
    20761 acaaaagaca atgacaagaa aaatggatta aatttattaa aaagtagtgc agtagccacg
    20821 ttaccgaata aggggacaaa ggaacttact gcaaaagcga aagatgatca aacgaataaa
    20881 gttgccaaac aagggcagta taaaaatcaa gatcctatcg ttttagtgca tggtttcaat
    20941 gggtttacag atgatattaa tccttcagtg ttagctcatt attggggcgg taataaaatg
    21001 aacattcgcc aagatttaga agaaaatggt tacaaagctt atgaagcaag tataagtgct
    21061 tttggaagta actatgaccg cgcagttgaa ctttattatt atatcaaagg cggtcgtgta
    21121 gattatggtg cagcacatgc agcaaaatat ggacatgaac gttatggaaa aacatacgaa
    21181 ggaatttaca aagactggaa accaggacag aaggtacacc tagttggaca tagtatgggc
    21241 ggtcaaacga tacgtcaact agaagaatta ctgcgtaatg gtaatcgtga agaaatagag
    21301 tatcaaaaga aacatggtgg cgaaatttct ccactattca aaggtaataa tgacaacatg
    21361 atttcatcaa ttactacttt aggaacacca cataatggta cacacgcatc agatttagct
    21421 ggtaatgaag ctttagtgag acaaatcgta tttgatatcg gtaaaatgtt tggtaataaa
    21481 aattcaagag tagacttcgg gttggctcaa tggggtctaa aacagaagcc aaatgaatca
    21541 tatattgatt atgtcaaacg cgttaaacaa tctaatttat ggaaatcaaa agataatgga
    21601 ttttacgatc tgacgcgtga gggtgcaaca gatttaaatc gtaaaacgtc gttgaaccct
    21661 aacattgtgt ataaaacata cactggtgaa gcaacgcaca aagcattaaa tagcgataga
    21721 caaaaagcag acttaaatat gtttttccca tttgtgatta ctggtaactt aatcggtaaa
    21781 gcaactgaaa aagaatggcg agaaaacgat ggtttagtat ccgttatttc ttctcaacat
    21841 ccatttaatc aagcttatac aaatgcgacg gataaaattc aaaaaggcat ttggcaagtg
    21901 acgcctacaa aacatgattg ggatcatgtt gactttgtag gacaagacag ttctgataca
    21961 gtgcgcacaa gagaagaatt acaagatttt tggcatcatt tagcagacga tttagtgaaa
    22021 actgaaaagg tgactgatac taagcaagca taatttataa agtaaaggga ggtatataca
    22081 atgacagcag acttctttca attaatcgga tcattattta gaattctaaa agaattattc
    22141 aagtaatatc ttggcggggc cccaacaaaa agaatttcga aaagaaattc tacaaacaat
    22201 gcaagttggg gtgggacgac gaaataaatt ttgcgaaaat atcatttctg tcccactccc
    22261 attggcattt acgaagttta aatgtgcaat tagaatatat gtataacatt attaaacacg
    22321 cggtaaaacg aagtcagtca attcaaactg atttcgccca ccgcgtgttt ttaacatagc
    22381 ttaataatta ataagcatta atgttcaatt tatatggttg ttttccaata ataaacctaa
    22441 agatatagaa ttcacgcaat atcataccga cacctataca taaccctaaa atgaatagta
    22501 gtgatatcgc tagaaagacc attgtattat cctcaaatat atttgtatat gcaaacaatg
    22561 agtctagaat gattggatgt aataaataaa taaagaatga gaaagcacta atcatttgaa
    22621 tcgtattaaa taacattgtt ttaaaatgcg tgcaaatacc caagataaca ataaacataa
    22681 tactattata tggtgttaat gaatatgaaa agctggtaac gttccaatag tctccatttg
    22741 ctaacgcaat aaacacaaaa taagtagcta cagctaatac aatcataata actaaataac
    22801 gttctaagaa atttaataca cgttcgtagt tataacccat atatgcacct aaaaagaaat
    22861 aaaaaatcca tccgaatatt atagtgtttt cacttaatgg ataatagtgt agcacggtat
    22921 cgtgaaacgc tgtgttgttc gtaaagtaat ataaaaatga ttgctgtaaa ataaaagata
    22981 acaataataa tattttactg ttgaataggt tatagttaat tttaaaaatg atataactca
    23041 aaataaagaa ttgcatgata acaacgataa aatagccata ccattgacct aataggacat
    23101 tttcaatgaa ttgtttattg aaacttgaat ctgttaataa tgattcacta taactgtaaa
    23161 acaatcccat taatatgtaa ggaataagta tatattttac gcgtgtagtt aagtatctat
    23221 aggtgacttt ttggtaattc aaggttgtca gtaactgtga caagataata aagcaaggtg
    23281 taccaaaaat cacaatatta cgaatgtaaa attgtaatac taaggatcct ccctccatat
    23341 tttcatgttt taaagtaatt tgtgtaagta aatgtgtgat aataataatt gcacatataa
    23401 tagcacgtaa atatacgagt tcaagtctaa tctttttcat ggaatccgtc ccatctctta
    23461 attaaatgct caaaagcatc atcactaatt aatattctag ggatgtaata atcattggag
    23521 ttcggagtga ctgctttttc ctctaatgaa aaaccgtatt ttaacccagc ttttttgatt
    23581 accggtaatt tatcgtcatt catcaagcca taaggatagg ctatagtttt ctgcgacttt
    23641 ttaaagtttt tagttagata tttttcactt ttgtttaaat cttttatgat tgtagcttca
    23701 gaagctttca ttaattttga cttattattt ttagataagt tatgcaaatc gtgggtatgt
    23761 gtttcaaatt cccataaccc agttttatac atttctttta gttctttttt actaatcata
    23821 tcgaggttgt gaaagttttc ttccccaaca tgacctgtga taataaaccc agttgccggt
    23881 attttatatt tttttaagat tggataagca ttttcataaa tagtttcatc catatcatca
    23941 aagttaatcc atacacttcg ttttggaaac ttaccttttt tcttgtaata taaaaattct
    24001 ttcaaggtta aaaatttagc atcatgtgat tttagccatt ttatttgaga ttcaaattgt
    24061 gattgactaa cactataatt tttaatttct ttactactag aaaagaagta aataaaatta
    24121 ttcagaaaat tcgcttttct tacacggtga taatttaatg ccagagcact attttcttta
    24181 tatttcagtt ttttaggtga atcgtcatct gcatttgcaa tatgatgacc atccagtgtg
    24241 cttacaggca atatgatcaa gatactcaac actaaaatta ttaattttct atacttcacg
    24301 attctcttcc tctctgccat ttttgaatca atatgctaat tgtaaaaaat acaaaaatga
    24361 taatcgcgaa aatgcccata gtttcaaata tatctaaaat ttcagtattt tcaatgttta
    24421 aagcaacacg tattgtattg atactttcgt catgaatttc aaatatagta ccaatataaa
    24481 cgagtagaac aactaaacaa tatatccaaa agacacacga tatagcgata agtgctgttt
    24541 ctcttacaat atttagcgat gattttagcg ttgggtattc cctctgtctg ggcttgacca
    24601 tgttgcgtaa ccacctttct tacgttttaa tgcttttgga aatgcgacaa gaactactgc
    24661 tgcgttaata atccagtata ctgtcggata ccaacttaca aatatgagtc cagccatatt
    24721 ctttttctcg tagcgactat caataaagag tgcgactgta aattgaataa cgtttataaa
    24781 agtcatagta aatgatgata gtagaaatat tgaaaaacta tatgtcataa atgtatagtc
    24841 taagaagttt gctgttatga acaaatagcc taaatataga agcactatat atacccataa
    24901 aattgagatg atttgctcaa acatcaaaat atataaagga aaccttttcg ttttcattgt
    24961 gctaaaaaag tctcgtagta atacttcgtg tcccccttga gcccatctca cgcgttgctt
    25021 ccaaagacct cccaatgttt ctggaaccaa catccaacac atggcaagcg gttcatactt
    25081 aatacgatat ccacgtaaat gcaatttcca agaaactgca atatcttcgg taatcatatc
    25141 agtatcccag tagccaacgt cgacaactgc acttttttta aatagagtga agacacccga
    25201 aatagtattg actgcgccag caagtgtctg acttcgctta atacagccaa ttaaacttgc
    25261 atattctatc gtttgaattt tacctaaaat agaactctta tttcgaattc taggattacc
    25321 tgtaactgca ccaagttttg gatcatgttt gaaattctca atcatataat atggtgcatc
    25381 ttgatcaacg atagtatctg catccaagca cattacataa tcatatgaag cctgtttaat
    25441 gccttgattg agtgcgttgg ctttacctct gttttcttgt aaatcgacga aaataaagtc
    25501 attattttct ttgattttat agatgagttc tgctgtatta tctgaacttc catcattaat
    25561 gataataatt tctttcttct cgtatttgag tgcaagaaca ttagacaacg tatcttcaat
    25621 cgtttcactt tcgttataac aggcaagtaa aaatgtaatg ccttctaatt catccacatt
    25681 tatgtcaggc ttcttgttca atgaatatct aatttctctg gtaaaataga aataaattga
    25741 accgacaatc cagtaaatag acataaatac aggataaaaa agcaaaaagt taaaaaattg
    25801 caatttcttt acctaccttt cgttagttag gttgtaagcc atatggtaat tgatagtatt
    25861 ttaatttgca atagattgtt gttataatta aacggaaata tttgtaattg caacttaatt
    25921 ttcctgtaac atagtgtgat taattttcag tagggggtta taaaaattga aggataagat
    25981 tattgataac gcaataacct tattttcaga gaaggggtat gacggtacaa cacttgatga
    26041 tatagctaaa agtgtaaata taaagaaagc gagtttatat taccattttg actcgaaaaa
    26101 aagtatttac gaacaaagtg ttaaatgctg ttttgattac cttaataata ttattatgat
    26161 gaatcaaaat aaatcgaact attcaattga tgctttatat caattcttat ttgagtttat
    26221 tttcgacatc gaagaaaggt atattagaat gtacgttcaa ttatctaata cgcctgagga
    26281 attttctgga aatatttacg gacaaataca agatttaaat caatcattaa gtaaagagat
    26341 agcaaaattc tatgatgaat caaagataaa gatgacaaaa gaagactttc agaatttaat
    26401 attgctgttt cttgaaagtt ggtatttgaa agcatccttt tcgcaaaaat ttggagcagt
    26461 ggaagaaagt aaaagtcaat tcaaagatga agtgtattcg ctactaaata tatttttgaa
    26521 gaaataattt ttgttactag tttgtaataa ttaacttact tttgtaaaaa aagacatgag
    26581 attatttttt taaatctata taaagttgac aatacaaatc gatattgaga atattaagat
//
LOCUS       DANBWH010000012        25057 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.012, whole genome shotgun sequence.
ACCESSION   DANBWH010000012 DANBWH010000000
VERSION     DANBWH010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 25057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25057
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.012"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            70..375
                     /locus_tag="PPY92_000349"
     CDS             70..375
                     /locus_tag="PPY92_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HDJ2624375.1"
                     /translation="MKLTKKQLKEYIEDYKKSDDILINLYIETYEFYCRLRDELKNSD
                     LMIEHTNKAGASNIIKNPLSIELTKTVQTLNNLLKSMGLTAAQRKKIVQEEGGFGDY"
     gene            365..2055
                     /locus_tag="PPY92_000350"
                     /pseudo
     CDS             365..2055
                     /locus_tag="PPY92_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500054.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            2060..3298
                     /locus_tag="PPY92_000351"
     CDS             2060..3298
                     /locus_tag="PPY92_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDJ2624376.1"
                     /translation="MNVIAKENIVTRIKKKLIDNWIDQSTSKLYDFSPWKNRSFWGVI
                     NNTLETNETIFSAITKLSNSMASLPLKMYEDYKVVNTEVSDLLTVSPNNSLSSFDFIN
                     QIETIRNEKGNAYVLIERDIYHQPSKLFLLNPNVVEMLIENQSRELYYSIHAATGNKL
                     IVHNMDMLHFKHIVASNMVQGISPIDVLKNTTDFDNAVRTFNLTEMQKPDSFMLKYGS
                     NVGKEKRQQVLEDFKQYYEENGGILFQEPGVEIEPLPKKYVSEDIVASENLTRERVAN
                     VFQLPSVFLNARSNTNFAKNEELNRFYLQHTLLPIVKQYEEEFNRKLLTKTDREKNRY
                     FKFNVKSYLRADSATQAEVYFKAVRSGYYTINDIREWEDLPPVEGGDKPLISGDLYPI
                     DTPLELRKSLKGGDKNVNES"
     gene            3282..4055
                     /locus_tag="PPY92_000352"
     CDS             3282..4055
                     /locus_tag="PPY92_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDJ2624377.1"
                     /translation="MSMKAKYFQMKRKSKSKGEIFIYGDIVSDKWFESDVTATDFKNK
                     LDELGDISEIDVHINSSGGSVFEGHAIYNMLKMHPAKINIYVDALAASIASVIAMSGD
                     TIFMHKNSFLMIHNSWVMTVGNAEELRKTADLLDKTDAVSNSAYLDKAKNLDQEQLKQ
                     MLDAETWLTAEEALSFGLIDEVLGANEIAASISKEQYKRFENVPEDLKKDVDKITKID
                     DVDTSELVETPKESMSLEEKEKREKIKRECEILKMTMSY"
     gene            4067..5230
                     /locus_tag="PPY92_000353"
     CDS             4067..5230
                     /locus_tag="PPY92_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDJ2624378.1"
                     /translation="MPTLYELKQSLGMIGQQLKNKNDELSQKATDPNIDMEDIKQLET
                     EKAGLQQRFNIVERQVQDIEEKEKAKVKDTGEAYQSLNDHEKMVKAKAEFYRHAILPN
                     EFEKPSMEAQRLLHALPTGNDSGGDKLLPKTLSKEIVSEPFAKNQLREKARLTNIKGL
                     EIPRVSYTLDDDDFITDVETAKELKLKGDTVKFTTNKFKVFAAISDTVIHGSDVDLVN
                     WVENALQSGLAAKERKDALAVSPKSGLEHMSFYNGSVKEVEGADMYDAIINALADLHE
                     DYRDNATIYMRYADYVKIISVLSNGTTNFFDTPAEKVFGKPVVFTDAAVKPIVGDFNY
                     FGINYDGTTYDTDKDVKKGEYLFVLTAWYDQQRTLDSAFRIAKAKENTGSLPS"
     gene            5299..5577
                     /locus_tag="PPY92_000354"
     CDS             5299..5577
                     /locus_tag="PPY92_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDJ2624379.1"
                     /translation="MSLEEIKLWLRIDYNFENDLIEGLIQSAKSELLLSGVPDYDKDD
                     LEYPLFCTAIKYIIARDYESRGYSNDQSRSKVFNEKGLQKMILKLKKW"
     gene            5589..5921
                     /locus_tag="PPY92_000355"
     CDS             5589..5921
                     /locus_tag="PPY92_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624380.1"
                     /translation="MEFNEFKDRAYFFQYINKGPYPDEEEKMKLYSCFCKIYNPSMKD
                     REILKATESKSGLTIIMRSSKIEYLPQTNHLVKIDRGLYSDKLFNIKEIRIDTPDIGY
                     NTVVLSEK"
     gene            5918..6319
                     /locus_tag="PPY92_000356"
     CDS             5918..6319
                     /locus_tag="PPY92_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624381.1"
                     /translation="MSVEIKGIPEVLKKLESVYGKQSMQAKSDRALNEASEFFIKALK
                     KEFESFKDTGASIEEMTKSKPYTKVGSQERAVLIEWVGPMNRKNIIHLNEHGYTRDGK
                     KYTPRGFGVIAKTLAANERKYREIIKKELAR"
     gene            6320..6715
                     /locus_tag="PPY92_000357"
     CDS             6320..6715
                     /locus_tag="PPY92_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HDJ2624382.1"
                     /translation="MNILNTIKEILLSDAELQTYINSRIYYYKVTENAETSKPFVVIT
                     PIYDLPSDFMSDKYLSEEYLIQIDVESSNNQKTIDITKRIRYLLYQQNLIQASSQLDA
                     YFEETKRYVMSRRYQGIPKNIYYKNQRIE"
     gene            6750..7391
                     /locus_tag="PPY92_000358"
     CDS             6750..7391
                     /locus_tag="PPY92_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDJ2624383.1"
                     /translation="MAEGQGSYKVGFKRLYVGVFNPEATKVVKRMTWEDEKGGTVDLN
                     ITGLAPDLVDMFASNKRVWMKKQGTNEVKSDMSIFNIPSEDLNTVIGRSKDKNGTSWV
                     GENTRAPYVTVIGESEDGLTGQPVYVALLKGTFSLDSIEFKTRGEKAEAPEPTKLTGD
                     WMNRKVDVDGTSQGIVYGYHEGKEGEAEFFKKVFVGYTDSEDHSEDSAGSLPS"
     gene            7483..7938
                     /locus_tag="PPY92_000359"
     CDS             7483..7938
                     /locus_tag="PPY92_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HDJ2624384.1"
                     /translation="MTKTLKVYKGDDVVASEQGEGKVSVTLSNLEADTTYPKGTYQVA
                     WEENGKESSKVDVPQFKTNPILVSGVSFTPETKSIMVNTDDNVEPNIAPSTATNKILK
                     YTSEHPEFVTVDENTGAIHGVAEGTSVITAMSTDGSDKSGQISVTVTNG"
     gene            7996..8346
                     /locus_tag="PPY92_000360"
     CDS             7996..8346
                     /locus_tag="PPY92_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624385.1"
                     /translation="MIKFEIKDRKTGKTESYTKEDVTMGEAEKCYEYLELVNQENKKE
                     APNATKMRQKERQLLVDLFKDEGLTEEDVLNKMSTKTYTKALQDIFREINGEDEEDSE
                     TEPEEMGKTEEQSQ"
     gene            8388..8546
                     /locus_tag="PPY92_000361"
     CDS             8388..8546
                     /locus_tag="PPY92_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624386.1"
                     /translation="MEQYGWTLTEVRKQPYVKLLEILNEENKEETEEKQSEQKVITGT
                     DLRKLFGS"
     gene            8560..14761
                     /locus_tag="PPY92_000362"
                     /pseudo
     CDS             8560..14761
                     /locus_tag="PPY92_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500042.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            14761..15585
                     /locus_tag="PPY92_000363"
     CDS             14761..15585
                     /locus_tag="PPY92_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HDJ2624387.1"
                     /translation="MQSFVKIIDGYKEEVITDFNQLIFLDARAESPNTNDNSVTINGV
                     DGILPGAISFAPFSLVLRFGYDGIDVIDLNLFEHWFRSVFNRRHPYYVITSQMPGVKY
                     AVNTANVTSNLKDGSSTEIEVSLNVYKGYSESVNWTDSEFLFDSNWMFENGIPLDFTP
                     KYTHTSNQFTIWNGSTDTINPRFKHDLKILINLNASGGFELVNYTTGDIFKYNKSIDK
                     NTDFVLDGVYAYRDINRVGIDTNRGIITLAPGKNEFKIKGDVSDIKTTFKFPFIYR"
     gene            15594..17177
                     /locus_tag="PPY92_000364"
     CDS             15594..17177
                     /locus_tag="PPY92_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDJ2624388.1"
                     /translation="MDYHDHLSVMDFNELICENLLDVDYGSFKEYYELNEARYITFTV
                     YRTTHNSFVFDLLICENFIIYHGEKYTIKQTAPKVEGDKVFIEVTAYHIMYEFQNHSV
                     ESNKLDDDSSETGKTPEYSLDEYLRYGFANQKTSVKMTYKIIGDFKRKVPIDELGNKN
                     GLEYCKEAVDLFGCIIYPNDTEIGFYSPETFYQRSEKVIRYQYNTDTVSATVSTLELR
                     TAIKVFGKKYTAEEKKNYNPIRTTDIKYSNGFIKEGTYRTETIGSKATINFDCKYGNE
                     TVRFTIKKGSQGGIYKLILDGKQIKQISCFAKSVQSETIDLIKNIDKGKHVLEMIFLG
                     EDPKNRIDISSNKKAKPCMYVGTEKSTVLNLIADNSGRNQYKAIVDYVADSAKQFGIR
                     YANTQTNEDIETQDKLLEFAKKQINDTPKTELDVNYIGYEKIEPRDSVFFVHELMGYN
                     TELKVVKLDRSHPFVNAIDEVSFSNEIKDMVQIQQALNRRVIAQDNRYNYQANRINHL
                     YTSTLNSPFETMDIGSVLI"
     gene            17177..17467
                     /locus_tag="PPY92_000365"
     CDS             17177..17467
                     /locus_tag="PPY92_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624389.1"
                     /translation="MATEEVKIKALLENDKQYFPATHWKAINGIPYAGSSDIDGLPQD
                     GIISVDDKNKLDKLKIGEAGIIQNSIVQKSPNGKLWKITVDDSGKLGTVLFY"
     gene            17483..19393
                     /locus_tag="PPY92_000366"
     CDS             17483..19393
                     /locus_tag="PPY92_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624390.1"
                     /translation="MENLYLIKDLGALAGRDYRAKEIQNLQRIEQFALGLTTEFKLHQ
                     KAKTMQHFAEQIYYNGRSQAAVNKSLQSQINALVVAPRNNSANEIVQARVNVNGETFD
                     TLKEHLDDWETKTQINKEETIRELNKTKQEILDIEYRFEPDKQEFLFVTELAPLTNAV
                     MQSFWFDNRTGIVYMTQARNNGYMLSRLRPNGQFIDSSLIVGGGHGTHNGYRYIDDEL
                     WIYSFILNGNNENTLVRFKYTPNVEISYGKYGMQDVFTGHPEKPYITPVINEKENKIL
                     YRIERPRSQWELENSMNYIEIRSLDDVDKNIDKVLHKISIPMRLTNETQPMQGVTFDE
                     KYLYWYTGDSNPNNRNYLTAFDLETGEEAYQVNADYGGTLDSFPGEFAEAEGLQIYYD
                     KDSGKKALMLGVTVGGDGNRTHRIFMIGQRGILEILHSRGVPFIMSDTGGRVKPLPMR
                     PDKLKNLGMLTEPGLYYLYTDHTVQIDDFPLPREWRDAGWFLEVKPPQTGGDVIQILT
                     RNSYARNMMTFERVLSGRTGDISDWNYVPKNSGKWERVPSFITKMSDINIVGMSFYLT
                     TDDTKRFTDFPTERKGVAGWNLYVEASNTGGFVHRLVRNSVTASAEILLKNYDSKTSS
                     GPWTLHEGRIIS"
     gene            19393..20859
                     /locus_tag="PPY92_000367"
     CDS             19393..20859
                     /locus_tag="PPY92_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HDJ2624391.1"
                     /translation="MSNLEKSVAINLENTAHYENISNLDITFRTGESDSSVLLFNIIK
                     NNQPLLLSEENIKARIAIRGKGVMVVAPLEILDPFKGILKFQLPNDVIKRDGSYQAQV
                     SVAELGNSDVVVVERTITFNVEKSLFSKIPSETKLHYIVEFQELEKTIMDRAKAMDEA
                     IKNGEDYASLIEKAKEKGLSDIQIAKSSSIDELKQLANSRISDLENKAQAYSRTFDEQ
                     KRYMDEKHEAFKQSVNSGGLVTSGSTSNWQKAKITKDDGKIMQITGFDFNNPEQRIGD
                     STQFIYVSQAINYPRDVSTNGTVEYLVVTSDYKRMTYRPNGTNKVFVKRKEAGSWSEW
                     SELAINDYNTPFETVQSAQSKANMAESNAKLYADDKFNKRYSVIFDGTANGVGSTLYL
                     NESLDQFILLIFYGTFPGGDFTEFGSPFGGGKISLNPSNLPDGDGNGGGVYEFGLTKS
                     SRTSLTISNDVYFDLGSQRGSGANANRGTINKIIGVRK"
     gene            20859..21248
                     /locus_tag="PPY92_000368"
     CDS             20859..21248
                     /locus_tag="PPY92_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2977 domain-containing protein"
                     /protein_id="HDJ2624392.1"
                     /translation="MQILVNKRNEIISYAIIGGFEEGIDIENLPENFSQVFRPKAFKY
                     SNGEIVFNEDYSEEKDDLHQQIDSEEQNTVASDDILRKMVASMQKQVVQSTKLSMQVN
                     KQNALMAKQLVTLNKKLEEVKGETENA"
     gene            21241..21405
                     /locus_tag="PPY92_000369"
     CDS             21241..21405
                     /locus_tag="PPY92_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDJ2624393.1"
                     /translation="MLKLISPTFEDIKTWYQLKEYSKEDIAWYVDMEVIDKEEYAIIT
                     GEKYPENLES"
     gene            21451..21750
                     /locus_tag="PPY92_000370"
     CDS             21451..21750
                     /locus_tag="PPY92_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HDJ2624394.1"
                     /translation="MFGFTKRHEQDWRLTRLEENDKTMFEKFDRIEDSLRTQEKIYDK
                     LDRNFEELRRDKEEDEKNKEKNAKNIRDIKMWILGLIGTILSTFVIALLKTIFGI"
     gene            21886..22188
                     /locus_tag="PPY92_000371"
     CDS             21886..22188
                     /locus_tag="PPY92_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDJ2624395.1"
                     /translation="MDAKVITRYIVLILALVNQFLANKGISPIPVDEESVSSIILTVV
                     ALYTTYKDNPTSQEGKWANQKLKKYKAESKYRKATGQAPIKEVMTPTNMNDTNDLG"
     gene            22199..23653
                     /locus_tag="PPY92_000372"
     CDS             22199..23653
                     /locus_tag="PPY92_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDJ2624396.1"
                     /translation="MLITKNQAEKWFDNSLGKQFNPDLFYGFQCYDYANMFFMIATGE
                     RLQGLYAYNIPFDNKARIEKYGQIIKNYDSFLPQKLDIVVFPSKYGGGAGHVEIVESA
                     NLNTFTSYGQNWNGKGWTNGVAQPGWGPETVTRHVHYYDDPMYFIRLNFPDKVSVGDK
                     AKSVIKQATAKKQAVIKPKKIMLVAGHGYNDPGAVGNGTNERDFIRKYITPNIAKYLR
                     HAGHEVALYGGSSQSQDMYQDTAYGVNVGNKKDYGLYWVKSQGYDIVLEIHLDAAGES
                     ASGGHVIISSQFNADTIDKSIQDVIKNNLGQIRGVTPRNDLLNVNVSAEINMNYRLSE
                     LGFITNKKDMDWIKKNYDLYSKLIAGAIHGKPIGGLVAGNVKTSAKNQKNPPVPAGYT
                     PDKNNVPYKKETGYYTVANVKGNNVRDGYSTNSRITGVLPNNTTITYDGAYCINGYRW
                     ITYIANSGQRRYIATGEVDKAGNRISSFGKFSTI"
     gene            24375..24590
                     /locus_tag="PPY92_000373"
     CDS             24375..24590
                     /locus_tag="PPY92_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624397.1"
                     /translation="MTALPSQYDDMFILNTQAHARLDQMAQQFEVVCNGLNENEGQAI
                     QTMDQSASLIRSNLIQVKEQLEKLAVY"
     gene            24680..>25057
                     /locus_tag="PPY92_000374"
     CDS             24680..>25057
                     /locus_tag="PPY92_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HDJ2624398.1"
                     /translation="MFKRTKLILIATILLSGCSTTNNESNNETKSVPEEMEASKYVGQ
                     GFQPPAEKDAIEFSKKHKDKIAKRGEQFFMDNFGLKVKATNVVGSGDGVEVFVHCDDH
                     DIVFNASIPFDKSIIESDSSLRSE"
BASE COUNT         9526 a         3432 c         5044 g         7055 t
ORIGIN      
        1 ttcgccgggt accggagagg cccaaacgct agcaacgcgg ataaattttt catgaaaggg
       61 ggtctttata tgaagttaac aaaaaaacag ctaaaagaat atatagaaga ttacaaaaaa
      121 tctgatgaca tattaattaa tttgtatata gaaacatatg aattttattg tcggttaaga
      181 gatgaactta aaaatagtga tttaatgata gagcatacaa acaaggctgg tgcgagcaat
      241 attattaaga atccattaag catagaactg acaaaaacag ttcaaacact aaataactta
      301 ctcaagtcta tgggtttaac tgcagcacaa agaaaaaaga tagttcaaga agaaggtgga
      361 ttcggtgact attaaagttt taaatgaacc ttcaccaaaa ctattaacaa catggtatgc
      421 agagcaagtc actcaaggga aaataaaaac aagcaaatat gttagaaaag aatgtgagag
      481 acatcttaga tatctagaaa atggaggtaa atgggtattt gatgaagaat tagcgcatcg
      541 tcctattcga tttatagaaa agttttgtaa accttccaaa ggatctaaac gtcaacttgt
      601 attacagcca tggcaacatt ttattatcgg cagtttgttt ggttgggttc ataaagaaac
      661 aaaactgcgc aggtttaaag aagctttgat atttatgggg cgaaaaaatg gtaaaacaac
      721 cactatttct ggggttgcta actatgctgt atcacaagat ggagaaaatg gtgcagaaat
      781 tcatttgtta gcaaacgtaa tgaaacaagc taggattcta tttgatgaat ctaaggcgat
      841 gattaaagct agcccaaagc ttgataaaaa tttcagaaca ttaagagatg aaatccatta
      901 tgacgcaacg atatcaaaaa ttatgcccca agcatcagat agcgataagt tagatggatt
      961 gaatacacac atggggattt ttgatgaaat tcatgaattt aaagactata aattgatttc
     1021 agttataaaa aactcaagag ctgcaaggtt acaacctctt ctcatctaca ttacgacagc
     1081 agggtatcaa ttagatggtc cacttgttga tatggtagaa gcgggaagag acaccttaga
     1141 tcaaatcata gaagacgaaa gaacttttta ttatttagca tctttggatg atgacgatga
     1201 tattaatgat tcgtcgaact ggataaaagc aaatcccaac ttaggtgtct ctataaattt
     1261 agatgagatg aaagaagagt gggaaaaagc taagagaaca ccagctgaac gtggagattt
     1321 tataaccaaa aggtttaata tctttgctaa taatgacgag atgagtttta ttgattaccc
     1381 aacactccaa aaaataatga aattgtttct ttagaagagc tggaaggcag accgtgcacg
     1441 attggttatg atttatcaga aacagaggac tttacagccg cgtgtgctac ttttgcgtta
     1501 gataatggta aagttgcagt tttatcgcat tcatggattc ctaagcacaa agttgaatat
     1561 tctaacgaaa aaatacccta tagagaatgg gaagaagatg gcttattaac agtgcaagat
     1621 aagccttata ttgactacca agatgtttta aattggataa ttaagatgaa tgagcattat
     1681 gtagtagaaa aaattactta tgatagagcg aacgcattca aactaaatca agagttaaaa
     1741 aattacgggt ttgaaacgga agaaacaaga caaggagctt tgaccttgag ccctgcattg
     1801 aaggatttaa aagaaatgtt tttagatggg aaaataatat ttaataataa tcctttaatg
     1861 aaatggtata tcaataatgt tcagttgaaa ctagacagaa acggaaactg gttgccgtct
     1921 aagcaaagca gatatcgtaa aatagatggt tttgcagcat ttttaaacac atatacagat
     1981 attatgaata aagttgtttc tgatagtggt gaaggaaaca tagagtttat tagtattaaa
     2041 gacataatgc gttaaggagg tgaatgttat cgcaaaagag aatattgtca cacgcataaa
     2101 gaaaaaattg atagacaatt ggattgatca gtcaacttct aagctttatg actttagccc
     2161 atggaaaaat agatcttttt ggggtgtaat taataatacg cttgaaacta atgaaacgat
     2221 attttcagct attacaaagt tatctaattc gatggctagt ttgcccttga aaatgtatga
     2281 agattataaa gtagttaata cagaagtatc tgatttactt acagtgtcac cgaataattc
     2341 tctgagcagt tttgatttta ttaatcaaat tgaaacaatc agaaatgaaa aaggtaatgc
     2401 atatgtgcta attgaacgag acatctatca tcaaccatca aagcttttct tattaaatcc
     2461 aaatgttgtt gaaatgttaa ttgaaaacca atcacgtgaa ctttattatt ccattcatgc
     2521 tgcaactgga aataaattga ttgttcataa tatggacatg ttgcatttta aacacatcgt
     2581 ggcatctaat atggtgcaag gcattagtcc gattgatgtg ttgaagaata caactgattt
     2641 tgataatgca gtaagaacct ttaatcttac agaaatgcaa aaacctgatt ctttcatgct
     2701 taaatatggt tccaatgtag gtaaagaaaa aaggcagcaa gtgttagaag atttcaaaca
     2761 gtactatgaa gaaaacggtg gaatattatt ccaagagcct ggtgttgaaa tcgaaccgtt
     2821 acctaaaaaa tatgtctctg aagatatagt ggcaagcgag aatttaacaa gagaaagagt
     2881 agctaacgtt tttcaattgc cctcagtatt cttaaatgca agatcaaata caaatttcgc
     2941 gaaaaatgaa gagttaaaca gattttactt gcagcatacc ttattgccaa tcgtcaaaca
     3001 gtatgaagaa gaatttaatc ggaaactact tactaaaaca gacagagaaa aaaataggta
     3061 ttttaaattt aacgttaaat cttatttaag ggctgatagt gcaacacaag cagaagtgta
     3121 ctttaaagca gttcgtagtg gttactacac tataaatgac attagagagt gggaagattt
     3181 accaccagtt gaaggtggag ataagccgct aataagcggt gatttatacc caattgacac
     3241 gccacttgaa ttaagaaaat ctttgaaagg tggtgataaa aatgtcaatg aaagctaagt
     3301 attttcaaat gaaaagaaaa tcaaaaagta aaggtgaaat atttatttat ggtgatattg
     3361 taagtgataa atggtttgaa agtgatgtaa ctgctacaga tttcaaaaat aaactagatg
     3421 aactaggaga catcagtgaa atagatgttc atataaattc atctggaggc agtgtatttg
     3481 aagggcatgc aatatacaat atgctaaaaa tgcatcctgc aaaaattaat atctatgtcg
     3541 atgccttagc ggcatcaatt gctagtgtta tcgctatgag tggtgacact atttttatgc
     3601 acaaaaatag ttttttaatg attcataatt catgggttat gactgtaggt aatgcagaag
     3661 aattaagaaa gacagcggat ttacttgata aaacagatgc tgttagtaat tcagcgtatt
     3721 tggataaagc aaaaaactta gatcaagaac aattaaagca gatgttagat gcagaaacat
     3781 ggcttactgc tgaagaagcc ttatcttttg gcttaataga tgaagtttta ggagctaatg
     3841 aaatagctgc tagtatctct aaagagcagt ataagcgttt cgagaacgtc ccggaagatt
     3901 taaagaaaga tgtagacaaa atcacaaaaa ttgatgatgt agatacatct gaattggttg
     3961 aaacacctaa agaaagtatg tcactagaag aaaaagaaaa aagagaaaaa attaaacgcg
     4021 aatgcgaaat tttaaaaatg acaatgagtt attaggagga aatgaaatgc cgacattata
     4081 tgaattaaaa caatcattag gtatgattgg acaacaatta aaaaataaaa atgatgagtt
     4141 gagtcagaaa gcaacagatc caaatattga tatggaagac atcaaacaac tagaaacaga
     4201 aaaagcaggc ttacaacaaa gatttaacat tgttgaaaga caagtacaag acattgaaga
     4261 aaaagaaaaa gcgaaagtta aagacacagg agaagcttat caatctttaa atgatcatga
     4321 gaagatggtt aaagctaagg cagagtttta tcgtcacgcg attttaccaa atgaatttga
     4381 aaaaccttca atggaggcac aacgtttatt acacgcttta ccaacaggta atgattcagg
     4441 tggagataag ctcttaccaa aaacactttc taaagaaatt gtttcagaac catttgctaa
     4501 aaaccaatta cgtgaaaaag ctcgtctaac taacattaaa ggtttagaga ttccaagagt
     4561 ttcatacact ttagacgatg atgatttcat tacagacgta gaaacagcaa aagaattaaa
     4621 attaaaaggt gatacagtca agttcactac taataaattc aaagtatttg ctgcaatttc
     4681 agatactgta attcatggat cagatgtaga tttagtaaac tgggttgaaa acgcactaca
     4741 atcaggatta gcagctaaag agcgtaaaga tgccttagca gtaagtccta aatctggatt
     4801 agaacacatg tcattttata atggatctgt taaagaagtt gagggagcag acatgtatga
     4861 tgctattatt aacgctttag cagatttaca tgaagattac cgtgataacg caacaattta
     4921 tatgcgatat gcagattatg tcaaaattat tagtgttctt tcaaatggaa caacaaattt
     4981 ctttgacaca ccagcagaaa aagtatttgg caaaccagta gtatttacag atgcagcagt
     5041 taaacctatt gtgggagatt tcaattattt tggaattaac tatgatggaa caacttatga
     5101 cactgataaa gatgttaaaa aaggcgaata tttgtttgta ttaacagcat ggtatgatca
     5161 gcaacgtaca ttagacagtg cattcagaat tgcaaaagca aaagaaaata caggttcatt
     5221 acccagctaa accccaaaag gttaatgtaa cagctaaggc taaatcagct gtaatatcag
     5281 ccgaataggg gtgatgaaat gagtttggaa gaaattaaat tgtggttgag aattgactat
     5341 aatttcgaaa atgatttaat tgaaggtctc attcaatcgg ctaagtctga attattatta
     5401 agtggggttc cagattatga caaagatgac ttggaatacc cgcttttttg tacagcgatt
     5461 aaatatatca ttgcaagaga ttatgaaagt cgtggatact caaatgacca atctagaagc
     5521 aaggtgttta atgaaaaagg attgcaaaaa atgattttga aattaaaaaa gtggtaggtg
     5581 atttttaaat ggaatttaat gaatttaaag atcgcgcgta tttttttcaa tatataaaca
     5641 aaggaccata tccagatgaa gaggaaaaaa tgaaattgta tagttgcttt tgtaaaattt
     5701 ataatccttc tatgaaagat agagaaattt taaaagcgac tgaatcaaag tcaggactaa
     5761 ccataattat gaggtcttct aaaattgaat atctaccaca aacaaatcac ttagttaaaa
     5821 ttgacagagg cttatattcc gataaattat tcaacattaa agaaataaga attgatacac
     5881 cagatattgg ctataataca gtggttttat cagaaaaatg agtgtagaaa ttaaagggat
     5941 acctgaagtg ttgaagaaat tagaatcggt atacggtaaa caatcaatgc aagctaagag
     6001 tgatagagct ttaaatgaag catctgaatt ttttataaag gctttaaaga aagaattcga
     6061 gagttttaaa gatacgggtg ctagcataga agaaatgact aaatctaagc cttatacaaa
     6121 agtaggaagt caagaaagag ctgttttaat tgaatgggta ggccctatga atcgcaaaaa
     6181 cattattcac ttgaatgaac atggttatac aagagatgga aaaaaatata caccaagagg
     6241 ttttggagtt attgcaaaaa cattagctgc taatgaacgg aagtatagag aaattataaa
     6301 aaaggagttg gccagataaa tgaatatatt aaacaccata aaagaaattt tattatctga
     6361 tgcagagctc caaacatata taaattctag aatatactat tataaagtca ctgaaaatgc
     6421 tgaaacttcc aaaccttttg ttgttattac acctatttat gatttacctt cagactttat
     6481 gtctgataaa tatcttagtg aagaatactt aattcaaata gatgtagaat cttcaaataa
     6541 tcagaaaaca attgatataa caaaacgaat aagatatctg ttatatcaac aaaatttaat
     6601 tcaagcatct agtcagttag atgcttattt tgaagaaact aaacgttatg tgatgtcgag
     6661 acgttatcaa ggcataccaa aaaatatata ttataaaaat cagcgcatcg aataggtgtg
     6721 ctttttaatt tttaaggagg aaataagcaa tggcagaagg acaaggttct tataaagtag
     6781 gttttaaaag attatacgtt ggagttttta acccagaagc aacaaaagta gttaaacgca
     6841 tgacatggga agatgaaaaa ggtggtacag ttgatctaaa tatcacaggt ttagcaccag
     6901 atttagtaga tatgtttgca tctaacaaac gtgtttggat gaaaaaacaa ggtactaatg
     6961 aagttaagtc tgacatgagt atttttaata ttccaagtga agatctaaat acagttattg
     7021 gtcgttctaa agataaaaat ggtacatctt gggtaggaga gaatacaaga gcaccatacg
     7081 taacagttat tggagaatct gaagatggtt taacaggtca accagtgtac gttgcgctac
     7141 ttaaaggtac ttttagcttg gattcaattg aatttaaaac acgaggagaa aaagcagaag
     7201 ccccagaacc tacaaaatta acaggtgact ggatgaatag aaaagttgat gttgatggaa
     7261 cgtcacaagg tattgtatac ggttatcatg aaggtaaaga aggagaagca gaattcttca
     7321 aaaaagtatt cgttggatac acggacagtg aagatcattc agaggattct gcaggttcgt
     7381 tacccagcta atccccaaaa tgttgaagta gcagttaatt caaaatctgc aacagtttca
     7441 gcagaatagg ggctttcaaa ataaatcaaa ggagaataat ttatgactaa aactttaaag
     7501 gtttataaag gagacgacgt cgtagcttct gaacaaggtg aaggcaaagt gtcagtaact
     7561 ttatctaatt tagaagcgga tacaacttat ccaaaaggta cttaccaagt ggcatgggaa
     7621 gaaaatggta aagaatctag taaagttgat gtacctcaat tcaaaaccaa tccaattcta
     7681 gtctcaggcg tatcatttac accagaaact aaatcaatta tggtaaatac cgatgacaat
     7741 gttgagccaa acattgcacc aagcacagca acgaataaaa tattgaaata tacaagtgaa
     7801 catccagaat ttgttactgt agatgaaaat acaggagcaa ttcacggtgt agctgaaggt
     7861 acttcagtaa tcactgctat gtctactgat ggaagcgata agtcaggaca aatttcagtg
     7921 acagtaacaa acggataggg atttaaggcg cagtatatct gcgtcttttt tatttgaata
     7981 aaaggagcta atacaatgat taaatttgaa attaaagatc gtaaaacagg aaaaacagag
     8041 agctatacaa aagaagatgt aacaatgggc gaagcagaaa aatgctatga gtatttagaa
     8101 ttagtaaatc aagagaataa aaaagaagca cctaacgcaa caaaaatgag acaaaaagag
     8161 cgacagttat tagtagattt atttaaagat gaaggattga ctgaagaaga tgttctgaac
     8221 aagatgagta ctaaaactta tacaaaagcc ttacaagata tatttcgaga aatcaatggt
     8281 gaagatgaag aagattcaga aactgaacca gaagagatgg gaaagacaga agaacaatct
     8341 caataaaaga cattttatcg aacattaaga aaatacaacg tttctgtatg gagcagtatg
     8401 ggtggacatt aactgaagtc agaaaacagc cgtatgtaaa acttttagaa atacttaatg
     8461 aagagaataa agaagagact gaagaaaaac aaagtgaaca aaaagtcatt acaggtacgg
     8521 atttaagaaa actttttgga agctagaaag gaggttaata tgaatgaaaa agtagaaggc
     8581 atgaccttgg agctgaaatt agaccattta ggtatccaag aaggcatgaa aggtttaaag
     8641 cgacaattag gtgttgttaa tagtgaaatg aaagctaatc tgtcagcatt tgataagtct
     8701 gaaaaatcaa tggaaaaata tcaggcgaga attaaggggt taaatgatag gcttaaagtt
     8761 caaaaaaaga tgtattctca agtagaagat gagcttaaac aagttaacgc taattaccaa
     8821 aaagctaaat ccagtgtaaa agatgttgag aaagtatatt taaagttagt agaagccaat
     8881 aaaaaagaaa aattagctct tgataaatct aaagaagcct taaaatcatc gaatacagaa
     8941 cttaaaaaag ctgaaaatca atataaacgt acaaatcaac gtaaacaaga tgcgtatcaa
     9001 aaacttaaac agttgagaga tgcagaacaa aagcttaaga atagtaacca agctactact
     9061 gcacaactaa aaagagcaag tgacgcagta cagaagcagt ccgctaagca taaagcactt
     9121 gttgaacaat ataaacaaga aggcaatcaa gttcaaaaac taaaagtgca aaatgacaat
     9181 ctttcaaaat caaatgataa aattgaaagt tcttacgcta aaactaatac taaattaaag
     9241 caaacagaaa aagaatttaa tgatttaaac aatactatta agaatcatag cgctaatgtc
     9301 gcaaaagctg aaacagctgt taataaagaa aaagctgctt taaataattt ggagcgttca
     9361 atagataaag cttcatccga aatgaagact tttaacaaag aacaaatgat agctcaaagt
     9421 catttcggta aacttgcaag tcaagcggat gtcatgtcaa agaaatttag ttctattgga
     9481 gacaaaatga cttccctggg acgtacaatg acgatgggcg tatctacacc aattacttta
     9541 gggttaggtg cagcattaaa aacaagtgca gactttgaag gccaaatgtc tcgagttgga
     9601 gcgattgcgc aagcaagcag taaagacttg aaaagcatgt ctaatcaagc agttgactta
     9661 ggagctaaaa ccagtaaaag tgctaacgaa gttgctaaag gtatggaaga attggcagct
     9721 ttaggcttta atgccaaaca aacaatggag gctatgccag gtgttatcag tgcagcagaa
     9781 gcaagtggtg cagaaatggc tacaactgca actgtaatgg cttcagcgat taactctttc
     9841 ggtttaaaag catctgatgc aaatcatgtt gctgatttac ttgcgagatc agcaaatgat
     9901 agtgctgcag atattcagta catgggagat gcattgaagt atgctggtac tcctgcaaaa
     9961 gcattaggag tttcaataga ggacacttcc gcagcaattg aagttttatc taactcaggt
    10021 ttagagggtt ctcaagcagg tactgcccta agagcttcat ttatcaggct agctaatcca
    10081 agtaaaaata cagctaagga aatgaaaaaa ttaggtattt atttgtctga tgctaaaggt
    10141 caatttgttg gcatgggtga attgattaga cagttccaag ataatatgaa aggcatgacg
    10201 agagaacaaa aactagctac agtggctaca atagttggta ctgaagcagc aagtggattt
    10261 ttagccttga ttgaagcggg accagataaa attaatagct atagtaaatc cttaaagaat
    10321 tccaatggcg aaagtaaaaa agcagcagat ttgatgaaag ataatctcaa aggcgctctg
    10381 gaacaattag gtggcgcttt tgaatcatta gcaatcgaag tcggtaaaga tttaacgcct
    10441 atgattagag caggagcgga aggtttaaca aaattagttg atggatttac acatctccct
    10501 ggttgggtta gaaaagcttc agtaggatta gcactttttg gtgcagcaat tggacctgca
    10561 gttcttgctg gagggttatt aatacgtaca gttggaagtg ctgctaaagg atatgcgtca
    10621 ttaaatagac gtattgctga aaatacaatc ctttcaaata ctaattcaaa agcaatgaaa
    10681 tctttaggtc ttcaaacatt atttcttggt tctacaacag gaaaaacgtc aaaaggcttt
    10741 aaagggttag ccggagctat gatgtttaat ttaaaaccta taaatgtttt gaaaaattct
    10801 gcaaagctag caattttacc gttcaaactt ttgaaaaacg gtttaggatt agctgcaaaa
    10861 tctttatttg cagtaagtgg aggcgcaaga tttgcgggtg tagccttaag gtttttaaca
    10921 ggacctatag gtgctacaat aactgctatt acaattgcgt ataaagtttt taaaaccgca
    10981 tatgatcgtg tggaatggtt cagaaacggt attaacggtt taggagaaac tataaagttt
    11041 tttggtggta aaattattgg cggcgctgtt agaaagctag gagagtttaa aaactatctt
    11101 ggaagtatcg ggcaaaagct tcaaagaaaa gttttcaaaa gatatgaaag atggttataa
    11161 atcattaagc gacgatgacc ttctcaaagt aggagtcaac aagtttaaag gatttatgca
    11221 aaccatgggc acagcttcta aaaaagcgtc tgatactgta aaagtgttag ggaaaggtgt
    11281 ttcaaaagaa acagaaaaag ctttagaaaa atatgtgcat tattctgaag aaaatagcag
    11341 aatcatggaa aaagtacgtt taaactcggg tcagatatca gaagacaaag caaaaaaact
    11401 tttgaaaatt gaaacggatt tatctaataa ccttatagct gaaatagaaa aaagaaataa
    11461 aaaggaactc gaaaaaactc aagaacttat tgataagtat agtgcattcg atgaacaaga
    11521 aaagcaaaac attttaacta gaactaaaga aaaaaatgac ttgcgaatta aaaaagagca
    11581 agaactcaat cagaaaatca aagaattgaa agaaaaagct ttaagtgatg gtcagatttc
    11641 agaaaatgaa agaaaagaaa ttgaaaagct tgaaaatcaa agacgtgaca tcactgttaa
    11701 agaattgagt aagactgaaa aagagcaaga gcgtatttta gtaagaatgc aaagaaacag
    11761 aaatgcttat tcaatagacg aagcgagcaa agcaattaaa gaagcagaaa aagcaagaaa
    11821 agcaagaaaa aaagaagtgg ataagcagta tgaagatgat gtcattgcta taaaaaataa
    11881 cgtcaacctt tctaagtctg aaaaagataa attgttagct attgctgatc aaagacataa
    11941 ggatgaagta agaaaggcaa aatctaaaaa agatgctgta gtagacgttg ttaaaaagca
    12001 aaataaagat attgataaag agatggattt atccagtggt cgtgtatata aaaatactga
    12061 aaagtggtgg aatggcctta aaagttggtg gtctaacttc agagaagacc aaaagaagaa
    12121 aagtgataag tacgctaaag aacaagaaga aacagctcgt agaaacagag aaaatataaa
    12181 gaaatggttt ggaaatgctt gggacggcgt aaaaactaaa actggcgaag cttttagtaa
    12241 aatgggcaga aatgctaatc attttggcgg cgaaatgaaa aaaatgtgga gtggaatcaa
    12301 aggaattcca agcaaattaa gttcaggttg gagctcagcc aaaagttctg taggatatca
    12361 cactaaggct atagctaata gtactggtaa atggtttgga aaagcttggc aatctgttaa
    12421 atcgactaca ggaagtattt acaatcaaac taagcaaaag tattcagatg cctcagataa
    12481 agcttgggcg cattcaaaat ctatttggaa agggacatca aaatggttta gcaatgcata
    12541 taaaagtgca aagggctggc taacggatat ggctaataaa tcgcgctcga aatgggataa
    12601 tatttctagt acagcatggt cgaatgcaaa atccgtttgg aaaggaacat cgaaatggtt
    12661 tagtaactca tacaaatctt taaaaggttg gactggagat atgtattcaa gagcccacga
    12721 tcgttttgat gcaatttcaa gttcggcatg gtctaacgct aaatcagtat ttaatggttt
    12781 tagaaaatgg ctatcaagaa catatgaatg gattagagat attggtaaag acatgggaag
    12841 agctgcggct gatttaggta aaaatgttgc taataaagct attggcggtt tgaatagcat
    12901 gattggcggt attaataaaa tatctaaagc cattactgat aaaaatctca tcaagccaat
    12961 acctacattg tctactggta ctttagcagg aaagggtgta gctaccgata attcgggagc
    13021 attaacgcaa ccgacatttg ctgtattaaa tgatagaggt tctggaaacg ccccaggcgg
    13081 tggagttcaa gaagtaattc acagggctga cggaacattc catgcacccc aaggacgaga
    13141 tgtggttgtt ccactaggag ttggagatag tgtaataaat gccaatgaca ctctgaagtt
    13201 acagcggatg ggtgttttgc caaaattcca tggtggtacg aaaaagaaaa aatggatgga
    13261 acaagttact gaaaatcttg gtaaaaaagc aggggacttc ggttctaaag ctaaaaacac
    13321 agctcataat atcaaaaaag gtgcagaaga aatggttgaa gccgcaggcg ataaaatcaa
    13381 agatggtgca tcttggttag gcgataaaat cggcgatgtg tgggattatg tacaacatcc
    13441 agggaaacta gtaaataaag taatgtcagg tttaaatatt aattttggag gcggagctaa
    13501 cgctacagta aaaattgcta aaggcgcgta ctcattgctc aaaaagaaat tagtagacaa
    13561 agtaaaatcg tggtttgaag attttggtgg tggaggcgat ggaagctatc tatttgacca
    13621 tccaatttgg caaaggtttg ggagctacac aggtggactt aactttaatg gcggtcgtca
    13681 ctatggtatc gactttcaaa tgcctactgg aacgaacatt tatgctgtta aaggcggtat
    13741 agctgataaa gtatggactg attacggtgg cggtaattct atacaaatta agaccggtgc
    13801 taacgaatgg aactggtata tgcatttatc taagcaatta gtaagacaag gccaacgtat
    13861 taaagctggt caactgatag ggaaatcagg tgctacaggt aatttcgtta gaggagcaca
    13921 cttacatttc caattgatgc aagggtcgca tccagggaat gatacagcta aagatccaga
    13981 aaaatggttg aagtcactta aaggtagtgg cgttcgaagt ggttcaggtg ttaataaggc
    14041 tgcatctgct tgggcaggcg atatacgtcg tgcagcaaaa cgaatgggtg ttaatgttac
    14101 ttcgggtgat gtaggaaata tcattagctt gattcaacac gaatcaggag gaaatgcagg
    14161 tataactcaa tctagttcgc ttagagacat caacgtttta cagggcaatc cagcaaaagg
    14221 attgcttcaa tatatcccac aaacatttag acattatgct gttagaggtc acaacaatat
    14281 atatagtggt tacgatcagt tattagcgtt ctttaacaac agatattggc gctcacagtt
    14341 taacccaaga ggtggttggt ctccaagtgg tccaagaaga tatgcgaatg gtggtttgat
    14401 tacaaagcat caacttgctg aagtgggtga aggagataaa caggagatgg ttatcccttt
    14461 aactagacgt aaacgagcaa ttcaattaac tgaacaggtt atgcgcatca tcggtatgga
    14521 tggcaagcca aataacatca ctgtaaataa tgatacttca acagttgaaa aattgttgaa
    14581 acaaattgtt atgttaagtg ataaaggaaa taaattaaca gatgcattga ttcaaactgt
    14641 ttcttctcag gataataact taggttctaa tgatgcaatt agaggtttag aaaaaatatt
    14701 gtcaaaacaa agtgggcata gagcaaatgc aaataattat atgggaggtt tgactaatta
    14761 atgcaatctt ttgtaaaaat catagatggt tacaaggaag aagtaataac agattttaat
    14821 cagcttatat ttttagatgc aagggctgaa agtccaaaca ccaatgataa cagtgtaact
    14881 attaacggag tagatggtat tttaccgggc gcaattagtt ttgcgccttt ttcattagta
    14941 ttaaggtttg gctatgatgg tatagatgtt atagatttaa atttatttga gcattggttt
    15001 agatctgtgt ttaatcgcag acatccttat tatgttatta cttctcaaat gcctggtgtt
    15061 aaatatgcag tgaatacagc taatgttaca tctaatttaa aagatggttc ttcaactgaa
    15121 attgaagtaa gtttaaatgt ttataaaggg tattctgaat cagttaattg gaccgatagc
    15181 gagttcttat tcgactctaa ttggatgttt gaaaatggaa ttcctcttga tttcacacct
    15241 aaatatactc atacatcaaa tcaatttact atttggaacg gttctactga tacgataaat
    15301 ccacgattca agcacgattt gaaaatatta attaatttaa atgcgagtgg aggatttgaa
    15361 ctggttaact atacaacagg tgatattttt aagtacaaca aaagtataga taaaaacact
    15421 gattttgttt tagatggtgt gtatgcatat cgagatataa atagagtggg aattgataca
    15481 aatagaggca ttataacatt agcgccaggt aaaaatgaat ttaagattaa aggagacgtc
    15541 agtgatatta aaactacatt taagtttcct tttatttata ggtaggtgat ttaatggatt
    15601 atcatgatca tttatcagta atggatttta atgaattgat ttgtgaaaat ttactagatg
    15661 tagattatgg ttcttttaaa gaatattatg aactgaatga agctaggtac atcaccttta
    15721 cagtttatag aactactcat aatagttttg tttttgattt attgatttgt gaaaacttca
    15781 taatttatca tggtgaaaaa tatacaatta agcagacagc gccaaaggtt gaaggtgata
    15841 aagtttttat tgaagttacg gcatatcaca taatgtatga atttcaaaat cactcagtgg
    15901 aatcaaataa gcttgatgac gacagtagcg aaactggtaa aacgccagaa tactctttag
    15961 atgagtactt aagatatgga tttgcaaatc aaaaaacttc ggtcaaaatg acctataaaa
    16021 taattggaga ttttaagcga aaagtaccga ttgacgaatt aggtaacaaa aacggcttag
    16081 aatactgtaa agaagcggta gacctgtttg gctgtataat ttacccaaat gatacagaga
    16141 ttggttttta ttctcctgaa acattttatc aaagaagcga gaaagtgatt cgatatcaat
    16201 ataatactga tactgtatct gcaactgtca gtacattgga attaagaaca gctataaaag
    16261 tttttggaaa aaagtataca gctgaggaaa agaaaaatta taatcctatt agaacaactg
    16321 acattaaata ttcaaatggt tttataaaag aaggtactta tcgtaccgaa acaattgggt
    16381 ctaaagctac tattaacttt gattgcaagt atggtaatga aacagttaga tttacaataa
    16441 aaaagggctc tcaaggtgga atatataagt tgattttaga cggcaagcaa attaagcaaa
    16501 tttcttgttt tgctaagtcg gttcagtctg aaacaataga tttaataaaa aatattgata
    16561 aaggcaagca cgttttagaa atgatatttt taggagaaga ccccaaaaat agaattgata
    16621 tatcttcaaa taaaaaagct aagccttgta tgtatgttgg aactgaaaaa tcaacagtct
    16681 taaatttaat tgctgataat tcaggtcgca atcaatacaa agcaattgtc gactacgtcg
    16741 cagatagtgc aaagcagttt gggattcgat atgctaatac gcaaacaaat gaagatatcg
    16801 aaacacagga taagctgtta gaatttgcaa aaaagcaaat aaatgatact cctaagactg
    16861 aattagatgt taattatata ggttatgaaa aaatagagcc aagagatagc gtattttttg
    16921 ttcatgaatt aatgggatat aacactgaat taaaggttgt taaacttgat aggtcacatc
    16981 catttgtaaa cgcaatagat gaagtgtctt tcagcaatga aataaaagat atggtacaaa
    17041 ttcaacaagc acttaacaga cgagttattg cacaagataa tagatataac tatcaagcaa
    17101 atcgtataaa tcatttatac actagtactt tgaattctcc tttcgagaca atggatatag
    17161 ggagtgtatt aatataatgg caacagaaga agttaaaatc aaagcgctac ttgaaaacga
    17221 taaacagtac tttccagcta cacactggaa agctataaat gggatacctt atgcaggcag
    17281 tagtgatatt gatggattgc ctcaagacgg tatcatttcg gtagatgata aaaataaatt
    17341 agataaatta aaaataggcg aagcaggaat tattcaaaat agcattgtac agaaatcccc
    17401 aaacggtaaa ttgtggaaaa taacagttga cgatagtggg aaacttggta cagtgctatt
    17461 ttattagaaa ggaaggtgca ttatggaaaa tttgtattta ataaaggatt tgggagcttt
    17521 agcaggtcga gattatagag ctaaagaaat tcaaaacctg caaagaatag agcaatttgc
    17581 gcttggcttg acaacagagt ttaagttgca tcagaaagct aaaacaatgc aacacttcgc
    17641 tgagcaaatt tattataatg gtagatcgca agcagcagta aacaaatctt tacaaagtca
    17701 aattaacgca cttgttgtgg caccacgtaa taacagtgct aatgagattg ttcaagctcg
    17761 agttaatgta aacggcgaaa cctttgacac attaaaagaa catttagacg attgggaaac
    17821 caaaactcaa attaataaag aggaaactat aagagaatta aataagacca aacaagaaat
    17881 tcttgatatc gagtatcgtt ttgaacctga taagcaagag tttttatttg tgacagaact
    17941 tgcacctctt acaaatgcag taatgcaatc cttctggttt gataatagaa caggcatagt
    18001 atacatgaca caagctagaa ataatggcta tatgctaagt cgtttaagac ctaatggtca
    18061 atttatagac agctcattga ttgtaggtgg gggtcatggt acacataacg gttatagata
    18121 tattgatgat gagttatgga tttatagttt tatcttaaat ggtaataatg agaatacatt
    18181 agttcgtttc aagtatacgc ctaatgtgga aattagctat ggcaagtatg gtatgcaaga
    18241 tgtatttaca ggacacccag aaaaacccta catcacccct gtcataaatg aaaaagaaaa
    18301 taaaattcta tacagaattg agagacctag aagtcagtgg gaacttgaaa actcaatgaa
    18361 ttatatagag ataagaagtt tagacgatgt tgataaaaat attgataaag ttttgcataa
    18421 aatcagtatc cctatgagac taacaaacga aacccaacca atgcagggtg tgacttttga
    18481 tgaaaaatac ttgtattggt atacaggaga cagtaatcca aataatagaa actatttaac
    18541 ggctttcgat ttagaaacag gagaagaagc gtatcaggtt aatgctgact atggtggaac
    18601 actagattca tttcctggcg aatttgcgga agcagaaggt ttgcaaatat actatgacaa
    18661 agatagtggt aaaaaagctt tgatgctagg tgttactgtc ggtggtgatg gaaatagaac
    18721 acatcgtatt ttcatgattg ggcaaagagg tattttagaa atacttcact caagaggcgt
    18781 tccttttatc atgagtgaca caggtggtag agttaaacct ttaccaatga ggcctgataa
    18841 acttaagaat cttgggatgt taacagagcc aggtctttac tatttataca ctgatcatac
    18901 agttcaaatc gatgatttcc cattaccaag agaatggcgt gatgcaggtt ggttcttgga
    18961 agttaagcca ccacaaactg gcggtgatgt aattcagata ttgacgcgta atagttatgc
    19021 aaggaatatg atgacttttg aaagggtgct ttctggaaga actggagaca tttcggactg
    19081 gaattatgtg cctaaaaata gtggtaaatg ggagagagta ccttcattca tcacaaaaat
    19141 gtcagatatt aacatagtag gcatgtcgtt ttatttaact acggatgata caaaacgttt
    19201 tacagatttt ccaactgaac gtaaaggggt agctggttgg aacttatatg tagaagcttc
    19261 aaacacaggt ggctttgttc ataggctagt tcgtaatagt gttacagcat ctgctgagat
    19321 actattgaaa aattatgata gtaaaacaag ttcagggcca tggactttac acgaagggag
    19381 aattataagt taatgagtaa tttagagaaa tctgtagcta taaatttaga aaacacagcg
    19441 cattatgaaa atatttcaaa tctagatata acttttagaa caggagagag tgattcttct
    19501 gttcttcttt ttaatatcat taaaaataat caaccgttat tactgagtga agaaaatatc
    19561 aaagcacgaa tagcgattcg aggtaaagga gtaatggtag ttgctccact agaaatatta
    19621 gatccattta aaggtatttt aaaatttcaa ttacctaatg atgtaattaa aagagatgga
    19681 agttatcaag ctcaagtttc ggttgcagaa ttaggtaatt cagacgtggt agttgtagag
    19741 agaactatca catttaacgt tgaaaaaagt ttgtttagca agattccctc tgaaacaaaa
    19801 ctacactata ttgttgagtt tcaagaatta gaaaaaacta ttatggatcg cgcgaaagca
    19861 atggacgagg ctataaaaaa tggtgaggat tatgcgagtc tgattgaaaa agctaaagaa
    19921 aaaggtctat cagatattca aatagcaaaa tcttcaagta ttgatgaatt aaagcaactt
    19981 gctaatagcc gtatatctga tttggaaaat aaagcgcaag catattcaag aacattcgat
    20041 gagcaaaagc gatatatgga tgagaaacat gaagccttca agcagtcagt gaatagtggt
    20101 ggtttagtca caagtggttc tacttcaaat tggcaaaaag ctaagattac taaagatgat
    20161 ggtaagataa tgcagattac tggatttgat tttaataatc cagaacaaag aataggtgat
    20221 tcaacccaat ttatttatgt ttcgcaagct ataaattatc caagagatgt tagtactaac
    20281 ggtactgtcg aatatttagt agtaacttca gattacaagc gtatgactta tcgaccgaac
    20341 ggtacaaata aagtgtttgt taaaagaaaa gaagcgggtt catggtctga gtggtcagaa
    20401 ttagctatta atgattacaa tacacctttt gaaactgttc aaagtgccca atcaaaagct
    20461 aatatggccg aaagtaacgc taaattatac gcagatgaca agtttaataa aaggtattcg
    20521 gttatttttg atggaacagc aaatggtgtg ggctctacat tgtacttaaa tgagagttta
    20581 gaccaattta ttttattaat tttttatggg acttttccag gtggtgactt tacagagttt
    20641 ggcagtcctt ttggaggagg aaagatttca ttgaatccct caaatcttcc agatggtgat
    20701 ggaaatggtg gaggtgttta tgagtttgga ttaactaaat ctagtcgtac atctttaact
    20761 atatcaaacg atgtctattt cgacttagga agtcaaagag gctctggtgc gaacgcaaat
    20821 agagggacaa ttaacaaaat tataggagtg agaaaataat gcaaatatta gttaacaagc
    20881 gtaatgagat aatttcatac gctatcattg gcggctttga agaaggtatt gatattgaaa
    20941 atttaccaga aaatttctct caagttttta gacctaaagc ctttaaatat tcaaatgggg
    21001 aaatagtttt taacgaagat tattcagaag aaaaagatga cttgcatcaa cagattgaca
    21061 gtgaagaaca aaacacagtc gcttctgatg acatcttacg aaaaatggtt gctagtatgc
    21121 agaaacaagt tgttcaaagt acaaagttat cgatgcaagt taataagcaa aatgcactaa
    21181 tggcaaaaca acttgtgaca cttaataaaa aattagaaga ggttaaagga gagactgaaa
    21241 atgcttaaat taatttcacc aacattcgaa gatattaaaa catggtatca attgaaagaa
    21301 tatagtaaag aagatatagc gtggtatgta gatatggaag ttatagataa agaggaatat
    21361 gcaattatta caggagaaaa gtatccagaa aatctagagt cataggttat aatcttatgg
    21421 ctttttaatt tgaataaagt gggtggtgta atgtttggat ttaccaaacg acacgaacaa
    21481 gattggcgtt taacgcgatt agaagaaaat gataagacta tgtttgaaaa attcgacaga
    21541 atagaagaca gtctgagaac gcaagaaaaa atttatgaca agttagatag aaatttcgaa
    21601 gaactaaggc gtgacaaaga agaagatgaa aaaaataaag agaaaaatgc taaaaatatt
    21661 agagacatca agatgtggat tctaggatta atagggacga ttctaagtac atttgttata
    21721 gccttgttaa aaactatttt tggcatttaa aggaggtgat caccatgctt aagggaattt
    21781 taggatatag cttttggtcg tgtttctggt ttagtaagtg taagtaatag ttaagagtca
    21841 gtgcttcggc actggctttt tattttggat aaaaggagca aacaaatgga tgcaaaagta
    21901 ataacaagat acatcgtatt gatcttagca ttagtaaatc aattcttagc gaataaaggt
    21961 ataagtccga taccagtaga tgaagaaagt gtttcatcga ttatcttaac agttgttgct
    22021 ttatatacta catataaaga taatccaaca tctcaagaag ggaaatgggc gaatcaaaaa
    22081 ttaaagaaat ataaagctga aagtaaatat agaaaagcaa cagggcaagc gccaattaaa
    22141 gaagtaatga cacctacgaa tatgaacgac acaaatgatt tagggtaggt gatgaccaat
    22201 gttgataaca aaaaaccaag cagaaaaatg gtttgataat tcattaggga agcagttcaa
    22261 tcctgatttg ttttatggat ttcagtgtta cgattacgca aatatgtttt ttatgatagc
    22321 aacaggcgaa aggttacaag gtttatacgc ttataatatt ccatttgata ataaagcaag
    22381 gattgaaaaa tacgggcaaa taattaaaaa ctatgatagc tttttaccgc aaaagttgga
    22441 tattgtcgtt ttcccgtcaa agtatggtgg cggagctgga catgttgaaa ttgttgagag
    22501 cgcaaattta aacactttca catcatatgg gcaaaattgg aatggtaaag gttggacaaa
    22561 tggcgttgcg caacctggtt ggggtcctga aactgttaca agacatgttc attattacga
    22621 tgacccaatg tattttatta gattaaattt cccagataaa gtaagtgttg gagataaagc
    22681 taaaagcgtt attaagcaag caactgccaa aaagcaagca gtaattaaac ctaaaaaaat
    22741 tatgcttgta gccggtcatg gttataacga tcctggagca gtaggaaacg gaacaaacga
    22801 acgcgatttt atccgtaaat atataacgcc aaatatcgct aagtatttaa gacatgccgg
    22861 tcatgaagtt gcattatatg gtggctcaag tcaatcacaa gacatgtatc aagatactgc
    22921 atacggtgtt aatgtaggca ataaaaaaga ttatggctta tattgggtta aatcacaggg
    22981 gtatgacatt gttctagaga ttcatttaga cgcagcggga gaaagtgcaa gtggtggaca
    23041 tgttattatc tcaagtcaat ttaatgcaga tactattgat aaaagtatac aagatgttat
    23101 taaaaataac ttaggacaaa taagaggtgt aacacctcgt aatgatttac taaacgttaa
    23161 tgtatcagca gaaataaata tgaattatcg tttatctgaa ttaggtttta ttactaataa
    23221 aaaagatatg gattggatta agaaaaacta tgacttgtat tctaaattaa tagctggtgc
    23281 gattcatggt aagcctatag gtggtttggt agctggtaat gttaaaacat cagctaaaaa
    23341 ccaaaaaaat ccaccagtgc cagcaggtta tacacccgat aaaaataatg taccgtataa
    23401 aaaagaaact ggttattaca cagtagctaa tgttaaaggt aataatgtaa gagacggtta
    23461 ttcaactaat tcaagaatta caggggtatt acccaacaac acaacaatta cgtatgacgg
    23521 tgcatattgt attaatggtt atagatggat tacttatatt gctaatagtg gacaacgtcg
    23581 ctatattgcg accggagagg tagacaaggc aggtaataga ataagtagtt ttggtaagtt
    23641 tagcacgatt tagtatttac ttagaataaa aattttgcta cattaattat agggaatctt
    23701 acagttatta aataactatt tggatggatg ttaatattcc tatacacttt ttaacattac
    23761 tctcaagatt taaatgtgcg taactggcag gtacttcggt acttgcctat ttttttatgt
    23821 tatagctagc cttcgggcta gttttttgtt atgatgtgtt acacatgcat caactattta
    23881 catctatcct tgttcaccca agcatgtcac tgggcgtttt ttccttgcga tagagagcat
    23941 agtttttata ctactccccg tagtatatat gactttagca ttcccgtata acagtttacg
    24001 gggtgctttt tatgttatac ttacttttat atagtaggag tgaactatat agccgggcag
    24061 aggccatgta tctgactgtt ggtcccacag gagacatctt ccttgtcatc actcgataca
    24121 tatatcttaa caacatagaa atgttacatt cgctacaacc gtatcttaat cgatacggtt
    24181 atatttattc ccctacaacc aacaaaacca cagatcctat taatttagga ttgtggttat
    24241 tttttgcgtt ttttcggggc gaaaaaaggg cagattattt gaaaaagggc aaacgcttgt
    24301 ggaaaatcta aaaggttaaa aatgacaaaa acattgatac aacagtgttt ttggacgctc
    24361 gtgtacgtta gagaatgacc gctttaccat cacaatatga tgatatgttt attttaaaca
    24421 cacaagctca tgcacgtctt gatcaaatgg cacaacagtt tgaagttgtt tgtaatggct
    24481 tgaacgaaaa tgaaggacaa gcaattcaaa cgatggatca atctgcctct ctaatacggt
    24541 caaacttaat tcaagttaaa gaacaattag aaaaactagc tgtatactaa gtaatttatt
    24601 aaatgctact tgttttcttt gagaataagt agttttttta acataaaagt tttacaaaca
    24661 cataaatggg tgatgagcta tgtttaaaag aacaaaacta atcttaatag caacgatact
    24721 actatcagga tgttcaacta ccaataacga atccaacaat gaaacaaaat cagtgccaga
    24781 agaaatggaa gcttcaaaat atgtaggaca aggcttccaa ccacctgcag aaaaagatgc
    24841 gattgaattt tcgaagaagc ataaagataa aattgctaaa cgtggcgaac aattttttat
    24901 ggataacttt ggactaaaag ttaaagctac aaatgttgta ggtagtggcg acggtgtaga
    24961 agtattcgtg cattgtgatg accacgacat cgtatttaat gcaagtattc catttgacaa
    25021 atcaataatt gagagtgata gctcattaag aagtgag
//
LOCUS       DANBWH010000013        24095 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.013, whole genome shotgun sequence.
ACCESSION   DANBWH010000013 DANBWH010000000
VERSION     DANBWH010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 24095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24095
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.013"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            202..1098
                     /gene="xerC"
                     /locus_tag="PPY92_000375"
     CDS             202..1098
                     /gene="xerC"
                     /locus_tag="PPY92_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDJ2624399.1"
                     /translation="MNHIQEAFLNTLKVERNFSEHTLKSYQDDLIQFNQFLEQEHLQL
                     KTFEYRDARNYLSYLYSNHLKRTSVSRKISTLRTFYEYWMTLDENIINPFVQLVHPKK
                     EKYLPQFFYEEEMEALFKTVEEDTSKNLRDRVILELLYATGIRVSELVNIKKQDIDFY
                     ANGVTVLGKGSKERFVPFGAYCRQSIENYLEHFKPIQSCNHDFLILNMKGEAITERGV
                     RYVLNDIVKRTAGVSEIHPHKLRHTFATHLLNQGADLRTVQSLLGHVNLSTTGKYTHV
                     SNQQLRKVYLNAHPRAKKENET"
     gene            1095..1640
                     /gene="hslV"
                     /locus_tag="PPY92_000376"
     CDS             1095..1640
                     /gene="hslV"
                     /locus_tag="PPY92_000376"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDJ2624400.1"
                     /translation="MSNTTLHATTIYAVRHNGKAAMAGDGQVTLGQQVIMKQTARKVR
                     RLYEGKVLAGFAGSVADAFTLFEKFETKLQQFSGNLERAAVELAQEWRGDKQLRQLEA
                     MLIVMDKDAILVVSGTGEVIAPDDDLIAIGSGGNYALSAGRALKRHASHLSAEEMAYE
                     SLKVAADICVFTNDNIVVETL"
     gene            1706..3109
                     /gene="hslU"
                     /locus_tag="PPY92_000377"
     CDS             1706..3109
                     /gene="hslU"
                     /locus_tag="PPY92_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HDJ2624401.1"
                     /translation="MDTAGIRLTPKEIVSKLNEYIVGQNDAKRKVAIALRNRYRRSLL
                     DEESKQEISPKNILMIGPTGVGKTEIARRMAKVVGAPFIKVEATKFTELGYVGRDVES
                     MVRDLVDVSVRLVKAQKKSLVQDEATAKANEKLVKLLVPSMKKKASQTNNPLESLFGG
                     AIPNFGQNNEDEEEPPTEEIKTKRSEIKRQLEEGKLEKEKVRIKVEQDPGALGMLGTN
                     QNQQMQEMMNQLMPKKKVEREVAVETARKILADSYADELIDQESANQEALELAEQMGI
                     IFIDEIDKVATNNHNSGQDVSRQGVQRDILPILEGSVIQTKYGTVNTEHMLFIGAGAF
                     HVSKPSDLIPELQGRFPIRVELDSLSVEDFVRILTEPKLSLIKQYEALLQTEEVTVNF
                     TDEAITRLAEIAYQVNQDTDNIGARRLHTILEKMLEDLSFEAPSMPNAVVDITPQYVD
                     DKLKSISTNKDLSAFIL"
     gene            3134..3907
                     /gene="codY"
                     /locus_tag="PPY92_000378"
     CDS             3134..3907
                     /gene="codY"
                     /locus_tag="PPY92_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDJ2624402.1"
                     /translation="MSLLSKTRELNTLLQKHKGIAVDFKDVAQTISSVTVTNVFIVSR
                     RGKILGSSLNELLKSQRIIQMLEERHIPSEYTERLMEVKQTESNIDIDNVLTVFPPEN
                     RELFIDSRTTIFPILGGGERLGTLVLGRVHDDFNENDLVLGEYAATVIGMEILREKHS
                     EVEKEARDKAAITMAINSLSYSEKEAIEHIFEELGGTEGLLIASKVADRVGITRSVIV
                     NALRKLESAGVIESRSLGMKGTFIKVKKEKFLDELEKSK"
     gene            3981..4055
                     /locus_tag="PPY92_000379"
     CDS             3981..4055
                     /locus_tag="PPY92_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624403.1"
                     /translation="MTLMIMLKSFLYFCLIFNQIDISH"
     gene            4254..5021
                     /gene="rpsB"
                     /locus_tag="PPY92_000380"
     CDS             4254..5021
                     /gene="rpsB"
                     /locus_tag="PPY92_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDJ2624404.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKKVDEAYNFLKQVSEDGGQVLFVGTKKQAQESVKSEAERAGQFYINQRWLGGLLT
                     NYKTISKRIKRISEIEKMEEDGLFEVLPKKEVVELKKEYDRLIKFLGGIRDMKSMPQA
                     LFVVDPRKERNAIAEARKLNIPIVGIVDTNCDPDEIDYVIPANDDAIRAVKLLTAKMA
                     DAILEGQQGVSNEEVAAEQNIDLDEKEKSEETEATEE"
     gene            5055..5168
                     /locus_tag="PPY92_000381"
     CDS             5055..5168
                     /locus_tag="PPY92_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624405.1"
                     /translation="MGDKILMLITFFKKKIEANYKYSIRVLAILPIIMLKS"
     gene            5203..6084
                     /locus_tag="PPY92_000382"
     CDS             5203..6084
                     /locus_tag="PPY92_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDJ2624406.1"
                     /translation="MATISAKLVKELRKKTGAGMMDCKKALTETDGDIDKAIDYLREK
                     GIAKAAKKADRIAAEGLVHVETKGNDAVIVEINSETDFVARNEGFQELVKEIANQVLD
                     TKAETVEALMETTLPNGKSVDERIKEAISTIGEKLSVRRFAIRTKTDNDAFGAYLHMG
                     GRIGVLTVVEGSTDEEAARDVAMHIAAINPKYVSSEQVSEEEINHEREVLKQQALNEG
                     KPENIVEKMVEGRLRKYLQEICAVDQDFVKNPDVTVEAFLKTKGGKLVDFVRYEVGEG
                     MEKREENFADEVKGQMK"
     gene            6221..6943
                     /locus_tag="PPY92_000383"
     CDS             6221..6943
                     /locus_tag="PPY92_000383"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDJ2624407.1"
                     /translation="MAQISKYKRVVLKLSGEALAGEKGFGINPVIIKSVAEQVAEVAK
                     MDCEIAVIVGGGNIWRGKTGSDLGMDRGTADYMGMLATVMNALALQDSLEQLDCDTRV
                     LTSIEMKQVAEPYIRRRAIRHLEKKRVVIFAAGIGNPYFSTDTTAALRAAEVEADVIL
                     MGKNNVDGVYSADPKVNKDAVKYEHLTHIQMLQEGLQVMDSTASSFCMDNNIPLTVFS
                     IMEEGNIKRAVMGEKIGTLITK"
     gene            6962..7516
                     /gene="frr"
                     /locus_tag="PPY92_000384"
     CDS             6962..7516
                     /gene="frr"
                     /locus_tag="PPY92_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDJ2624408.1"
                     /translation="MSDIINETKSRMQKSIESLSRELANISAGRANSNLLNGVTVDYY
                     GAPTPVQQLASINVPEARLLVISPYDKTSVADIEKAIIAANLGVNPTSDGEVIRIAVP
                     ALTEERRKERVKDVKKIGEEAKVSVRNIRRDMNDQLKKDEKNGDITEDELRSGTEDVQ
                     KATDNSIKEIDQMIADKEKDIMSV"
     gene            7889..8659
                     /locus_tag="PPY92_000385"
     CDS             7889..8659
                     /locus_tag="PPY92_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDJ2624409.1"
                     /translation="MFKKLINKKNTINNYNEELDSSNIPEHIAIIMDGNGRWAKKRKM
                     PRIKGHYEGMQTIKKITRIASDIGVKYLTLYAFSTENWSRPESEVNYIMNLPVNFLKT
                     FLPELIEKNVKVETIGFTDKLPKSTIEAINNAKEKTANNTGLKLIFAINYGGRAELVH
                     SIKNMFDELHQQGLNSDIIDETYINNHLMTKDYPDPELLIRTSGEQRISNFLIWQVSY
                     SEFIFNQKLWPDFDEDELIKCIKIYQSRQRRFGGLSEE"
     gene            8666..9448
                     /locus_tag="PPY92_000386"
     CDS             8666..9448
                     /locus_tag="PPY92_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDJ2624410.1"
                     /translation="MKVRTLTAIIALIVFLPILLKGGLVLMIFANILALIALKELLNM
                     NMIKFVSVPGLISAVGLIIIMLPQHAGPWVQVIQLKSLIAMSFIVLSYTVLSKNRFSF
                     MDAAFCLMSVAYVGIGFMFFYETRSEGLHYILYAFLIVWLTDTGAYLFGKMMGKHKLW
                     PVISPNKTIEGFIGGLFCSLIVPLAMLYFVDFNMNVWILLGVTLILSLFGQLGDLVES
                     GFKRHFGVKDSGRILPGHGGILDRFDSFMFVLPLLNILLIQS"
     gene            9660..10946
                     /gene="rseP"
                     /locus_tag="PPY92_000387"
     CDS             9660..10946
                     /gene="rseP"
                     /locus_tag="PPY92_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDJ2624411.1"
                     /translation="MSYLVTIIAFIIVFGVLVTVHEYGHMFFAKRAGIMCPEFAIGMG
                     PKIFSFRKNETLYTIRLLPVGGYVRMAGDGLEEPPVEPGMNVKIKLNEENEITHIILD
                     DHHKFQQIEAIEVKKCDFKDDLFIEGITAYDNERHHFKIARKSFFVENGSLVQIAPRD
                     RQFAHKKPWPKFLTLFAGPLFNFILALVLFIGLAYYQGTPTSTVEQVADKYPAQQAGL
                     QKGDKIVQIGKYKISEFDDVDKALDKVKDNKTTVKFERDGKTKSVELTPKKTERKLTK
                     VSSETKYVLGFQPASEHTLFKPIVYGFESFLKGSTLIFTAVVGMLASIFTGGFSFDML
                     NGPVGIYHNVDSVVKAGIISLIGYTALLSVNLGIMNLIPIPALDGGRILFVIYEAIFR
                     KPVNKKAETTIIAIGAIFMVVIMILVTWNDIRRYFL"
     gene            10966..12669
                     /locus_tag="PPY92_000388"
     CDS             10966..12669
                     /locus_tag="PPY92_000388"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDJ2624412.1"
                     /translation="MKQSKVFIPTMRDVPSEAEAQSHRLLLKSGLIKQSTSGIYSYLP
                     LATRVLNNITAIVRQEMERIDSVEILMPALQQAELWEESGRWGAYGPELMRLQDRHGR
                     QFALGPTHEELVTSIVRNELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDA
                     YSFHADEASLDQTYQDMYQAYSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGE
                     DTIVYSKESDYAANIEKAEVVYEPNHKHSTVQPLEKIETPNVKTAQELADFLGRPVDE
                     IVKTMIFKVDGEYIMVLVRGHHEINDIKLKSYFGTDNIELATQDEIVNLVGANPGSLG
                     PVIDKEIKIYADNFVQDLNNLVVGANEDGYHLINVNVGRDFNVDEYGDFRFILEGEKL
                     SDGSGVAHFAEGIEVGQVFKLGTKYSESMNATFLDNQGKAQPLIMGCYGIGISRTLSA
                     IVEQNHDDNGIVWPKSVTPFDLHLISINPKKDDQRELADALYAEFNTKFDVLYDDRQE
                     RAGVKFNDADLIGLPLRIVVGKRASEGIVEVKERLTGDSEEVHIDDLMTVITNKYDNL
                     K"
     gene            12933..17243
                     /locus_tag="PPY92_000389"
     CDS             12933..17243
                     /locus_tag="PPY92_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDJ2624413.1"
                     /translation="MTEQQKFKVLADQIKISNQLDAEILNSGELTRIDVSNKNRTWEF
                     HITLPQFLAHEDYLLFINAIEQEFKDIANVTCRFTVTNGTNQDEHAIKYFGHCIDQTA
                     LSPKVKGQLKQKKLIMSGKVLKVMVSNDIERNHFDKACNGSLIKAFRNCGFDIDKIIF
                     ETNDNDQEQNLASLEAHIQEEDEQSARLATEKLEKMKAEKAKQQDNNESAVDKCQIGK
                     PIQIENIKPIESIIEEEFKVAIEGVIFDINLKELKSGRHIVEIKVTDYTDSLVLKMFT
                     RKNKDDLEHFKALSVGKWVRAQGRIEEDTFIRDLVMMMSDIEEIKKATKKDKAEEKRV
                     EFHLHTAMSQMDGIPNIGAYVKQAADWGHPAIAVTDHNVVQAFPDAHAAAEKHGIKMI
                     YGMEGMLVDDGVPIAYKPQDVVLKDATYVVFDVETTGLSNQYDKIIELAAVKVHNGEI
                     IDKFERFSNPHERLSETIINLTHITDDMLVDAPEIEEVLTEFKEWVGDAIFVAHNASF
                     DMGFIDTGYERLGFGPSTNGVIDTLELSRTINTEYGKHGLNFLAKKYGVELTQHHRAI
                     YDTEATAYIFIKMVQQMKELGVLNHNEINKKLSNEDAYKRARPSHVTLIVQNQQGLKN
                     LFKIVSASLVKYFYRTPRIPRSLLDEYREGLLVGTACDEGELFTAVMQKDQSQVEKIA
                     KYYDFIEIQPPALYQDLIDRELIRDTETLHEIYQRLIHAGDTAGIPVIATGNAHYLFE
                     HDGIARKILIASQPGNPLNRSTLPEAHFRTTDEMLNEFHFLGEEKAHEIVVKNTNELA
                     DRIERVVPIKDELYTPRMEGANEEIRELSYTNARKLYGEDLPQIVIDRLEKELKSIIG
                     NGFAVIYLISQRLVKKSLDDGYLVGSRGSVGSSFVATMTEITEVNPLPPHYICPNCKT
                     SEFFNDGSVGSGFDLPDKTCETCGAPLIKEGQDIPFETFLGFKGDKVPDIDLNFSGEY
                     QPNAHNYTKVLFGEDKVFRAGTIGTVAEKTAFGYVKGYLNDQGIHKRGAEIDRLVKGC
                     TGVKRTTGQHPGGIIVVPDYMDIYDFTPIQYPADDQNSAWMTTHFDFHSIHDNVLKLD
                     ILGHDDPTMIRMLQDLSGIDPKTIPVDDKEVMQIFSTPESLGVTEDEILCKTGTFGVP
                     EFGTGFVRQMLEDTKPTTFSELVQISGLSHGTDVWLGNAQELIKTGICDLSSVIGCRD
                     DIMVYLMYAGLEPSMAFKIMESVRKGKGLTEEMIETMKENEVPDWYLDSCLKIKYMFP
                     KAHAAAYVLMAVRIAYFKVHHPLYYYASYFTIRASDFDLITMIKDKTSIRNTVKDMYS
                     RYMDLGKKEKDVLTVLEIMNEMAHRGYRMQPISLEKSQAFEFIIEGDTLIPPFISVPG
                     LGENVAKRIVEARDDGPFLSKEDLNKKAGLSQKIIEYLDELGSLPNLPDKAQLSIFDM
                     "
     gene            17533..18000
                     /gene="rimP"
                     /locus_tag="PPY92_000390"
     CDS             17533..18000
                     /gene="rimP"
                     /locus_tag="PPY92_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDJ2624414.1"
                     /translation="MSKITEQVEVIVQPIMEDLNFELVDVEYVKEGRDHFLRISIDKE
                     GGVDLNDCTLASEKISEAMDANDPIPEMYYLDVASPGAERPIKKEQDFQNAITKPVFV
                     SLYVPIEGEKEWLGILQEVNNETIVVQVKIKARTKDIEIPRDKIAKARHAVMI"
     gene            18021..19196
                     /gene="nusA"
                     /locus_tag="PPY92_000391"
     CDS             18021..19196
                     /gene="nusA"
                     /locus_tag="PPY92_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDJ2624415.1"
                     /translation="MSSNELLLATEYLEKEKKIPRAVLIDAIEAALITAYKKNYDSAR
                     NVRVELNMDQGTFKVIARKDVVEEVFDDRDEVDLSTALVKNPAYEIGDIYEEDVTPKD
                     FGRVGAQAAKQAVMQRLRDAEREILFEEFIDKEEDILTGIIDRVDHRYVYVNLGRIEA
                     VLSEAERSPNEKYIPNERIKVYVNKVEQTTKGPQIYVSRSHPGLLKRLFEQEVPEIYD
                     GTVIVKSVAREAGDRSKISVFSENNDIDAVGACVGAKGARVEAVVEELGGEKIDIVQW
                     NEDPKVFVKNALSPSQVLEVIVDETNQSTVVVVPDYQLSLAIGKRGQNARLAAKLTGW
                     KIDIKSETDAREAGIYPVVEAEKVTEEDIALEDADTTESTEEVNDVSVETNVEKESE"
     gene            19217..19501
                     /locus_tag="PPY92_000392"
     CDS             19217..19501
                     /locus_tag="PPY92_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDJ2624416.1"
                     /translation="MKKKKIPMRKCILSNEMHPKKDMIRVVVNKEGEIFADVTGKKQG
                     RGAYVSKDVAMVEKAQQKEILEKYFKASKEQLDPVYKEIIRLIYREEIPK"
     gene            19498..19815
                     /locus_tag="PPY92_000393"
     CDS             19498..19815
                     /locus_tag="PPY92_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ family RNA-binding protein"
                     /protein_id="HDJ2624417.1"
                     /translation="MSIDQILNFLGLAMRAGKVKTGESVIVNEIKKGNLKLVIVANDA
                     SDNTAKLITDKCKSYKVPFRKFGNRNELGIALGKGERVNVGITDPGFAKKLLSMIDEY
                     HKE"
     gene            19820..21937
                     /gene="infB"
                     /locus_tag="PPY92_000394"
     CDS             19820..21937
                     /gene="infB"
                     /locus_tag="PPY92_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDJ2624418.1"
                     /translation="MSKQRIYEYAKELNLKSKEIIDELKSMNIEVSNHMQALEDDQIK
                     ALDKKFKKEQKNDNKQSTQNNHQKSNNQNQNKGQQKDNKKNQQQNNKGNKGNKKNNRN
                     NKKNNKNNKPQNQPAAPKEIPSKVTYQEGITVGEFADKLNVESSEIIKKLFLLGIVAN
                     INQSLNQETIELIADDYGVEVEEEVVINEEDLSIYFEDEKDDPEAIERPAVVTIMGHV
                     DHGKTTLLDSIRHTKVTAGEAGGITQHIGAYQIENDGKKITFLDTPGHAAFTTMRARG
                     AQVTDITILVVAADDGVMPQTIEAINHAKEAEVPIIVAVNKIDKPTSNPDRVMQELTE
                     YGLIPEDWGGETIFVPLSALSGDGIDDLLEMIGLVAEVQELKANPKNRAVGTVIEAEL
                     DKSRGPSASLLVQNGTLNVGDAIVVGNTYGRIRAMVNDLGQRIKTAGPSTPVEITGIN
                     DVPQAGDRFVVFSDEKQARRIGESRHEASIVQQRQESKNVSLDNLFEQMKQGEMKDLN
                     VIIKGDVQGSVEALAASLMKIDVEGVNVRIIHTAVGAINESDVTLANASNGIIIGFNV
                     RPDSGAKRAAEAENVDMRLHRVIYNVIEEIESAMKGLLDPEFEEQVIGQAEVRQTFKV
                     SKVGTIAGCYVTEGKITRNAGVRIIRDGIVQYEGELDTLKRFKDDAKEVAKGYECGIT
                     IENYNDLKEGDVIEAFEMVEIKR"
     gene            22323..22673
                     /gene="rbfA"
                     /locus_tag="PPY92_000395"
     CDS             22323..22673
                     /gene="rbfA"
                     /locus_tag="PPY92_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDJ2624419.1"
                     /translation="MSSMRAERVGEQMKKELMDIINNKVKDPRVGFITITDVVLTNDL
                     SQAKVFLTVLGNDKEVENTFKALDKAKGFIKSELGSRMRLRIMPELMYEYDQSIEYGN
                     KIERMIQDLHKQDR"
     gene            22842..23759
                     /gene="truB"
                     /locus_tag="PPY92_000396"
     CDS             22842..23759
                     /gene="truB"
                     /locus_tag="PPY92_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDJ2624420.1"
                     /translation="MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAG
                     VLPVCIGNATRVSDYVMDMGKAYEATVSIGRSTTTEDQTGDTLETKGVHSADFNKDDI
                     DRLLENFKGVIEQIPPMYSSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDF
                     KENECHFKIRVICGKGTYIRTLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQI
                     KELHEQDSLQNKLFPLEYGLKGLPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVF
                     IDDDSEKVLAIYMVHPTKESEIKPKKVFN"
     gene            23774..>24095
                     /locus_tag="PPY92_000397"
     CDS             23774..>24095
                     /locus_tag="PPY92_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDJ2624421.1"
                     /translation="MKVIEVTHPIQSKQYITEDVAMAFGFFDGMHKGHDKVFDILNEI
                     AEARSLKKAVMTFDPHPSVVLNPKRKRTTYLTPLSDKIEKISQHDIDYCIVVNFSSRF
                     ANVSV"
BASE COUNT         8872 a         3279 c         4763 g         7181 t
ORIGIN      
        1 tttaaaagac aatgaaataa taaatggcaa ggctattaat aaagactttt agtaattaat
       61 ttaaaaaaga ggtattctaa ttaacaggtt ttccgattag ttacaattat ttaattctca
      121 aaagatttag aattgattat caaattactg taagcccttt gctgtatatg ctacaattct
      181 tattgatgga gggtaaatgt attgaatcat attcaagaag cgtttttaaa tacattgaaa
      241 gttgaacgga atttttcgga acatacattg aaatcatatc aagatgactt aattcagttt
      301 aatcaatttt tagaacaaga acatttacag ttgaaaactt ttgaatacag agatgctaga
      361 aattatttga gctatttata ttcaaatcat ttgaaaagaa catctgtttc tcgtaaaatc
      421 tcaacgttaa gaactttcta tgaatattgg atgacgcttg atgagaacat tattaatcca
      481 tttgttcaat tagtacatcc gaaaaaagaa aaatatcttc cgcaattctt ttacgaagaa
      541 gaaatggaag cgttattcaa aactgtagaa gaggacactt caaaaaattt acgggatcga
      601 gttattcttg aattgttgta tgctacaggc atccgtgttt cggaattagt aaatattaaa
      661 aaacaagata tagattttta cgcgaatggt gttaccgtat taggaaaagg gagcaaagag
      721 cgttttgtac cgtttggtgc ttattgtaga caaagcatcg aaaattattt agaacatttc
      781 aaaccaattc agtcatgcaa tcatgatttt cttattttaa atatgaaggg tgaagcaatc
      841 actgaacgcg gtgtacgata tgttttaaat gatattgtta aacgaacagc aggcgtaagt
      901 gagattcatc cccacaagct cagacataca tttgcaacgc atttattgaa tcaaggtgca
      961 gacctaagaa cagtacaatc gttattaggt catgttaatt tgtcaacaac tggtaaatat
     1021 acacacgtat ctaaccaaca attaagaaaa gtgtatctaa atgcacatcc tcgagcgaaa
     1081 aaggagaatg aaacatgagt aatacaacat tacatgcaac aacaatttat gctgtaagac
     1141 ataatgggaa agcagctatg gctggagatg gacaagtaac gcttggtcaa caagtcatca
     1201 tgaaacaaac ggcaagaaaa gtgcgacgtt tatatgaagg taaagtgtta gctggtttcg
     1261 caggtagtgt agcagatgcg tttacgttat ttgaaaaatt tgaaacaaaa ttacaacagt
     1321 ttagtggtaa cttagaaaga gctgctgttg aattggcaca agaatggcga ggcgataaac
     1381 aattacgtca attagaagct atgctaattg taatggataa agatgctatt ttagttgtca
     1441 gtggaactgg cgaagttatt gccccagatg atgaccttat cgctattgga tcagggggca
     1501 actacgcatt aagcgcagga cgtgcattga aacgccatgc atcgcatttg tctgctgaag
     1561 aaatggcata tgagagcttg aaagtagcgg ctgatatttg tgtctttacc aacgataata
     1621 ttgttgttga aacactataa taatcagagc acgataaata attacgacca attaatttta
     1681 gttaaaagac ggaggaatga aattaatgga tacagctgga ataagattaa ctccaaaaga
     1741 aatcgtatct aaattaaatg aatatatcgt tggacaaaat gatgctaaac gtaaagtggc
     1801 aattgcccta cgtaatcgat acagaagaag tttattagat gaggaatcaa agcaagaaat
     1861 ttcacctaaa aatattttga tgattggacc aaccggcgtt ggtaaaactg aaattgcaag
     1921 aagaatggcc aaagttgtcg gcgcgccatt tataaaagta gaagctacta aatttactga
     1981 gctaggttat gtaggacgag atgttgaaag tatggttaga gatcttgttg atgtttcagt
     2041 aagattagtc aaggcgcaga aaaaatcatt ggtacaagat gaagcaacag ctaaggccaa
     2101 tgaaaaactt gttaagttat tagttccaag tatgaaaaag aaagcgtctc aaacgaataa
     2161 tcctttagag tcacttttcg gaggtgcaat tccaaatttc ggacaaaata acgaagatga
     2221 agaagaacca cctactgagg aaattaaaac aaaacgttct gaaattaaga gacagctaga
     2281 agaaggcaaa cttgaaaaag aaaaggtaag aattaaagtc gaacaagatc ctggtgcttt
     2341 aggtatgcta ggtacaaatc aaaatcagca aatgcaagag atgatgaatc aattaatgcc
     2401 taaaaagaaa gttgagcgag aagttgctgt tgagacggca aggaaaatct tagctgatag
     2461 ttatgcggat gaactaattg atcaagaaag cgctaaccaa gaagcgcttg aattagcaga
     2521 acaaatgggt atcatcttta tagatgaaat cgacaaagtt gcgacgaata atcataatag
     2581 tggtcaagat gtctcaagac aaggtgttca aagagatatt ttacctatac ttgaaggtag
     2641 cgttattcaa accaaatatg gtactgtgaa tactgaacat atgctgttta taggtgctgg
     2701 agctttccat gtatctaagc cgagtgactt gataccagaa ttgcaaggtc gttttccgat
     2761 tagagttgaa cttgatagtt tatcggtaga agattttgta agaattttga cagaaccaaa
     2821 attgtcatta attaaacaat atgaagcatt gcttcaaaca gaagaagtta ctgtaaactt
     2881 taccgatgaa gcaattactc gcttagctga gattgcttat caagtaaatc aagatacaga
     2941 caacattggt gcacgtcgac ttcatacaat tttagaaaag atgctagaag atttatcatt
     3001 cgaagcacca agtatgccga atgcagttgt agatattacc ccacaatatg ttgatgataa
     3061 attaaaatca atttcaacaa ataaagattt aagtgcattt attctataaa aatatacaaa
     3121 aggagaaaaa ttcatgagct tattatctaa aacgagagag ttaaacacgt tacttcaaaa
     3181 acacaaaggt attgcggttg attttaaaga tgtagcacaa acgattagta gcgtaactgt
     3241 aacaaatgta tttattgtat cgcgtcgagg taaaatttta gggtcgagtc taaatgaatt
     3301 attaaaaagt caaagaatta ttcaaatgtt ggaagaaaga catattccaa gtgaatatac
     3361 agaacgatta atggaagtta aacaaacaga atcaaatatt gatatcgaca atgtattaac
     3421 agtattccca cctgaaaaca gagaattatt catagatagt cgtacaacta tcttcccaat
     3481 tttaggtgga ggagaaagat taggtacatt agtacttggt cgagtacatg atgattttaa
     3541 tgaaaatgat ttggtactag gtgaatatgc tgctacagtt attggtatgg aaatcttacg
     3601 tgagaagcat agtgaagtag aaaaagaagc gcgcgataaa gctgctatta caatggcaat
     3661 taattcatta tcttattctg aaaaagaagc gattgaacat atctttgaag aacttggcgg
     3721 tacggaaggc ctattaatcg catcaaaagt tgcagataga gttggtatta ctagatctgt
     3781 aattgtaaat gcactacgta aattagaaag tgctggtgta attgaatcac gttctttagg
     3841 aatgaaaggt actttcatta aagttaaaaa agaaaaattc ttagatgaat tagaaaaaag
     3901 taaataagtc gatgagtctg ggacataatt caatgtccta gtcgtgacga tgttatcttg
     3961 gcagtagtca actcaacgaa atgactttga tgattatgtt gaagtcattt ctttattttt
     4021 gtctaatttt caatcaaatt gatatttcac attaacttga tagaattacc tttaaattta
     4081 tggaaaaatg cttgaaaaac atttgcttta gacacggcat tatgttatat ttattttcgg
     4141 ctataaagcc aatacacaca tatcaaacgt gattgtgtaa agggtgcaat atttatatat
     4201 tgtctttaca atagtttgct atggaggtaa ttaaccaata ggaggaattt ataatggcag
     4261 taatttcaat gaaacaatta ctagaagcgg gtgttcactt cggtcaccaa acacgtcgtt
     4321 ggaacccaaa aatgaaaaaa tatatcttca ctgagagaaa tggtatttat atcatcgact
     4381 tacaaaaaac agtgaaaaaa gtagacgagg catacaactt cttgaaacaa gtttcagaag
     4441 atggtggaca agtcttattc gtaggaacta aaaaacaagc acaagaatca gttaaatctg
     4501 aagcagaacg tgctggtcaa ttctacatta accaaagatg gttaggtgga ttattaacta
     4561 actataaaac aatctcaaaa cgaatcaaac gtatttctga aattgaaaaa atggaagaag
     4621 atggtttatt cgaagtatta cctaaaaaag aagtagtaga acttaaaaaa gaatacgacc
     4681 gtttaatcaa attcttaggc ggaattcgtg atatgaaatc aatgcctcaa gcattattcg
     4741 tagttgaccc acgtaaagag cgtaatgcaa ttgctgaagc tcgtaaatta aatattccta
     4801 tcgtaggtat cgttgacact aactgtgatc ctgacgaaat tgactacgtt atcccagcaa
     4861 acgacgatgc tatccgtgcg gttaaattat taactgctaa aatggcagat gcaatcttag
     4921 aaggtcaaca aggcgtttct aatgaagaag tagctgcaga acaaaacatc gatttagatg
     4981 aaaaagaaaa atcagaagaa acagaagcaa ctgaagaata atcaactgtt gaatctgact
     5041 tagatatagt ttaaatgggt gataagatat taatgcttat cacctttttt aaaaagaaaa
     5101 tcgaggcaaa ttacaaatat tcaattagag tattggcaat cttgcctata ataatgctaa
     5161 aatcataata tataaaatga taacttattg gaggaataat gaatggcaac tatttcagca
     5221 aaacttgtta aagaattacg taaaaaaact ggcgcgggta tgatggattg taaaaaagcg
     5281 ctaactgaaa ctgatggtga catcgataaa gcgattgatt acctacgtga aaaaggtatt
     5341 gctaaagcag ctaaaaaagc agaccgtatt gcggctgaag gtttagtaca tgtagaaact
     5401 aaaggtaacg acgcagttat cgttgaaatc aactctgaaa cagactttgt tgctcgtaac
     5461 gaaggattcc aagagttagt taaagaaatc gctaatcaag tattagatac aaaagctgaa
     5521 actgttgaag ctttaatgga aacaacttta ccaaatggta aatcagttga tgaaagaatt
     5581 aaagaagcaa tttcaacaat cggtgaaaaa ttaagtgttc gtcgttttgc tatcagaact
     5641 aaaactgata acgatgcttt cggcgcttac ttacacatgg gtggacgcat tggtgtatta
     5701 acagttgttg aaggttcaac tgacgaagaa gcagcaagag acgttgctat gcatatcgct
     5761 gcaatcaacc ctaaatatgt ttcttctgaa caagttagcg aagaagaaat caaccacgaa
     5821 agagaagttt taaaacaaca agcattaaat gaaggtaaac cagaaaacat cgttgaaaaa
     5881 atggtggaag gacgtttacg taaatactta caagaaattt gtgctgtaga tcaagacttc
     5941 gttaaaaacc ctgatgtaac agttgaagct ttcttaaaaa caaaaggtgg aaaacttgtt
     6001 gacttcgtac gctatgaagt aggcgaaggt atggaaaaac gcgaagaaaa ctttgcggat
     6061 gaagttaaag gacaaatgaa ataatctgtc ataaagtaaa acaaggaaga agacaccttt
     6121 aatgttgctt tattaaaatg taaatcattc taataaaacg acaactgtgt cttctttact
     6181 tgtatatgtt acatatattc acgatagaga ggataagaaa atggctcaaa tttctaaata
     6241 taaacgtgta gttttgaaac taagtggtga agcgttagct ggagaaaaag gatttggcat
     6301 aaatccagta attattaaaa gtgttgctga gcaagtggct gaagttgcta aaatggactg
     6361 tgaaatcgca gtaatcgttg gtggcggaaa catttggaga ggtaaaacag gtagtgactt
     6421 aggtatggac cgtggaactg ctgattacat gggtatgctt gcaactgtaa tgaatgcctt
     6481 agcattacaa gatagtttag aacaattgga ttgtgataca cgagtattaa catctattga
     6541 aatgaagcaa gtggctgaac cttatattcg tcgtcgtgca attagacact tagaaaagaa
     6601 acgcgtagtt atttttgctg caggtattgg aaacccatac ttctctacag atactacagc
     6661 ggcattacgt gctgcagaag ttgaagcaga tgttatttta atgggcaaaa ataatgtaga
     6721 tggtgtatat tctgcagatc ctaaagtaaa caaagatgcg gtaaaatatg aacatttaac
     6781 gcatattcaa atgcttcaag aaggtttaca agtaatggat tcaacagcat cctcattctg
     6841 tatggataat aacattccgt taactgtttt ctctattatg gaagaaggga atattaaacg
     6901 tgctgttatg ggtgaaaaga taggtacgtt aattacaaaa taaatttaga ggtgtaaaat
     6961 aatgagtgac attattaatg aaactaaatc aagaatgcaa aaatcaatcg aaagcttatc
     7021 acgtgaatta gctaatatca gtgcaggaag agctaattca aatttattaa acggcgtaac
     7081 agttgattac tatggtgcac caacacctgt acaacaatta gcaagcatca atgttccaga
     7141 agcacgttta cttgttattt ctccatacga caaaacttct gtagctgaca tcgaaaaagc
     7201 gataatagcg gctaacttag gtgtcaaccc aacaagtgat ggtgaagtga tacgtattgc
     7261 agtacctgcc ttaacagaag agcgtagaaa agagcgcgtt aaagatgtta agaaaattgg
     7321 tgaagaagct aaagtatctg ttcgaaatat tcgtcgtgat atgaatgatc agttgaaaaa
     7381 agatgaaaaa aatggcgaca ttactgaaga tgagttgaga agtggtactg aagatgttca
     7441 gaaagcaaca gacaattcaa taaaagaaat tgatcaaatg attgctgata aagaaaaaga
     7501 tattatgtca gtataaaact aatatacaat gacatattaa aatgccagta ttaaacgata
     7561 atgtaacatt taaaatgggc atgtttaatt aaatcaaaga tgcatgtgat aatttaaatt
     7621 cacaatgagc ataaaaatgg tgtttaaaca agttaattaa acatatactt tataaataat
     7681 aggcattagg tatattgcta taataaagtt atgtaatttt taacctcagt atgtatgtca
     7741 catttctggt gtaaactgta ccgagtcaga ctttggtaca gtttttttat ttgcttattc
     7801 aatgcattaa atgagtatga taaaatgata atgattgttt agtaacttat attatatgac
     7861 agagatgatc aggctcggag gaaagaccat gtttaaaaag ctaataaata aaaagaacac
     7921 tataaataat tataatgaag aattagactc gtctaatata cctgaacata tcgctattat
     7981 tatggatggt aatgggcgat gggctaagaa gcgaaaaatg cctagaatta aaggccatta
     8041 cgaaggtatg caaacaataa aaaaaattac taggatagct agtgatattg gtgttaagta
     8101 cttaacttta tatgcctttt ccactgaaaa ttggtcaaga cctgaaagtg aagtaaatta
     8161 tattatgaat ttgcctgtca atttcttaaa gacattctta ccggaactaa ttgaaaaaaa
     8221 tgtcaaagtt gaaacaattg gatttactga taagttgcca aaatcaacga tagaagcaat
     8281 taataatgct aaagaaaaga cagctaataa taccggctta aaattaatat ttgcaattaa
     8341 ttatggtggc agagcagaac ttgttcatag tattaaaaat atgtttgacg agcttcatca
     8401 acaaggttta aatagtgata tcatagatga aacatatata aacaatcatt taatgacaaa
     8461 agactatcct gatccagagt tgttaattcg tacttcagga gaacaaagaa taagtaattt
     8521 cttgatttgg caagtttcgt atagtgaatt tatctttaat caaaaattat ggcctgactt
     8581 tgacgaagat gaattaatta aatgtataaa aatttatcag tcacgtcaaa gacgctttgg
     8641 cggattgagt gaggagtagt atagtatgaa agttagaacg ctgacagcta ttattgcctt
     8701 aatcgtattc ttgcctatct tgttaaaagg cggccttgtg ttaatgatat ttgctaatat
     8761 attagcattg attgcattaa aagaattgtt gaatatgaat atgattaaat ttgtttcagt
     8821 tcctggttta attagtgcag ttggtcttat catcattatg ttgccacaac atgcagggcc
     8881 atgggtacaa gtaattcaat taaaaagttt aattgcaatg agctttattg tattaagtta
     8941 tactgtctta tctaaaaaca gatttagttt tatggatgct gcattttgct taatgtctgt
     9001 ggcttatgta ggcattggtt ttatgttctt ttatgaaacg agatcagaag gattacatta
     9061 catattatat gcctttttaa ttgtttggct tacagataca ggggcttact tatttggtaa
     9121 aatgatgggc aaacataagc tttggccagt aataagtccg aataagacaa tcgaaggatt
     9181 cataggtggc ttgttctgta gtttgatagt gcctcttgca atgttatatt ttgtagattt
     9241 caatatgaat gtatggatat tacttggagt gacattgatt ttaagtttat ttggtcaatt
     9301 aggtgattta gtggaatcag gatttaagcg tcatttcggc gttaaagact caggtcgaat
     9361 actacctgga cacggtggta ttttagaccg atttgacagc tttatgtttg tgttaccatt
     9421 attaaatatt ttattaatac aatcttaatg ctgagaacaa atcaataaac gtaaagagga
     9481 gttgctgaga taatttaatg aatctcagaa ctccttttga aaattatacg caatattaac
     9541 tttgaaaatt agacgttata ttttgtgatt tgtcagtatc atattataat gacttatgtt
     9601 acgtatacag caatcaattt taaaataaaa gaaatttata aacaatcgag gtgtagcgag
     9661 tgagctattt agttacaata attgcattta ttattgtttt tggtgtacta gtaactgttc
     9721 atgaatatgg acatatgttt tttgcgaaaa gagcaggcat tatgtgtcca gaatttgcga
     9781 tcggtatggg gccaaaaatt tttagtttta gaaaaaatga aacactttac actattaggt
     9841 tattgcctgt tggtggatat gttcgtatgg caggagatgg cttagaagag ccaccagtcg
     9901 agcccggtat gaacgttaaa attaaactga atgaagaaaa tgaaataaca catatcatat
     9961 tagatgatca tcataagttt caacaaattg aagcgatcga agttaaaaaa tgtgatttta
    10021 aggatgactt attcatagaa ggtatcactg cttatgataa tgaaagacat cattttaaaa
    10081 ttgctagaaa gtctttcttt gttgaaaatg gtagcttagt tcaaattgct ccgagagaca
    10141 gacaatttgc acataaaaag ccatggccga aatttttaac attatttgcg ggaccgttat
    10201 ttaactttat attagcttta gtcctattta ttggtcttgc atattatcaa ggtacaccta
    10261 cgtctactgt agaacaagtc gcagataagt atccagctca acaagcagga ttacaaaaag
    10321 gtgataaaat tgttcaaatt ggcaaatata aaatatctga atttgatgat gttgataagg
    10381 cgttagataa agttaaagat aataagacga ctgttaaatt tgaacgtgat ggtaaaacga
    10441 agtcagttga attaacacct aaaaagactg aaagaaaact gactaaagta agttcagaga
    10501 cgaagtatgt tctcggattc caaccagcga gtgaacatac acttttcaaa ccaattgtat
    10561 atggatttga aagctttttg aaaggtagta cactaatttt tacagctgta gtcggtatgt
    10621 tggctagtat atttacgggc ggattctcat ttgatatgtt aaatggtccg gttggtattt
    10681 atcataacgt cgactcagtt gttaaagcgg gtatcattag cttaattggt tacactgcgt
    10741 tattaagtgt aaacttaggt attatgaatt taattcctat tcctgcacta gacggtggtc
    10801 gtattttatt tgttatatat gaagcgattt tcagaaaacc agttaataaa aaagcggaaa
    10861 caacgattat tgctattggt gccattttca tggtcgttat tatgatatta gtaacgtgga
    10921 atgatattcg acgatatttc ttataattta ggaggataaa taattatgaa gcaatccaaa
    10981 gtttttatac caacgatgcg tgatgtgcca tcagaagcag aagcacaaag tcatcgttta
    11041 ttattgaaat cgggtttgat aaaacaaagt acaagtggga tttatagtta tttaccgcta
    11101 gcaacacgtg tgttaaataa tattactgca attgtgcgac aagaaatgga acgtatcgat
    11161 tctgttgaaa ttttaatgcc agcgttacaa caagctgaat tatgggaaga atcaggacgt
    11221 tggggtgcat atggcccaga attaatgcgt ttacaagata gacatggaag acaatttgca
    11281 ttaggtccaa cacatgaaga attagttaca tcaatagtaa gaaatgaatt gaaatcatac
    11341 aaacaattac cgatgacatt attccaaatt caatctaaat tccgtgatga aaagagacca
    11401 cgttttggtt tacttcgtgg gcgtgaattt attatgaaag atgcgtattc attccatgct
    11461 gacgaggcat cattagatca aacgtatcaa gatatgtatc aagcgtatag ccgtattttt
    11521 gagagagttg gcattaatgc tagaccagtt gtagcagatt caggtgctat aggcggtagc
    11581 catacacatg aatttatggc attaagtgct atcggtgagg atacaatcgt ttacagtaaa
    11641 gaaagtgact atgctgctaa tatcgaaaaa gcagaagtcg tttacgaacc aaatcataag
    11701 cattctactg tgcaaccttt agaaaaaatt gaaacaccaa atgttaagac tgcacaagaa
    11761 ttggcagact tcttaggtag accagtagat gaaatcgtta aaacgatgat tttcaaagtt
    11821 gatggcgaat atattatggt tttagtgcgt ggccatcatg aaattaatga cattaaatta
    11881 aaatcttatt tcggcacaga taatattgaa ttagcaacac aagacgaaat tgttaattta
    11941 gttggtgcaa atccgggttc actaggtcct gttattgata aagaaatcaa aatttatgca
    12001 gataattttg tgcaagattt aaataattta gttgtcggtg ctaacgaaga tggctatcac
    12061 ttaattaatg taaatgtagg tagagacttc aacgttgatg aatatggcga tttccgtttt
    12121 attttagaag gcgaaaagtt aagtgatggt tcaggcgttg cacattttgc tgaaggtatt
    12181 gaagttggtc aagtattcaa attgggtact aagtattcag aatcaatgaa tgctacattc
    12241 ttagataacc aaggaaaagc tcaaccttta attatgggct gttacggtat tggaatttct
    12301 agaacgctaa gtgcgattgt tgaacaaaat cacgatgata atggaattgt ttggcctaaa
    12361 tcagttactc catttgattt acatttaatt tctattaatc ctaagaaaga tgatcaacga
    12421 gagctagcag atgcactata tgctgaattt aatactaaat ttgatgtgtt gtacgatgat
    12481 cgtcaggaac gtgcaggtgt caaatttaat gatgccgatt taattggttt accactgcga
    12541 attgttgttg gtaaacgtgc atcggaaggt attgtagaag ttaaagaacg tttaacaggt
    12601 gatagcgaag aagttcacat tgatgactta atgactgtca ttacaaataa atatgataac
    12661 ttaaaataat taagatcgaa tgaattataa gagtaggaaa aagctgaaag aaatctgatg
    12721 cttatgtcct gctcttatta tttttgatat aatgattatt cgatgaaaaa tgactgaaga
    12781 catagtataa ttaaagataa atttgtttta acaatattat gattagccaa atataaagca
    12841 tttaattttc tatcattact atgctcacat aatctaaata ttgttcgaac acgtaaaagt
    12901 aatttctatt taaggtggta attgtcttgg caatgacaga gcaacaaaaa tttaaagtgc
    12961 ttgctgatca aattaaaatt tcaaatcaat tagatgctga aattttaaat tcaggtgaac
    13021 tgacacgtat agatgtttct aacaaaaaca gaacatggga atttcatatt acattaccac
    13081 aattcttagc tcatgaagat tatttattat ttataaatgc aatagagcaa gagtttaaag
    13141 atatcgccaa cgttacatgt cgttttacgg taacaaatgg cacgaatcaa gatgaacatg
    13201 caattaaata ctttgggcac tgtattgacc aaacagcttt atctccaaaa gttaaaggtc
    13261 aattgaaaca gaaaaagctt attatgtctg gaaaagtatt aaaagtaatg gtatcaaatg
    13321 acattgaacg taatcatttt gataaggcat gtaatggaag tcttatcaaa gcgtttagaa
    13381 attgtggttt tgatatcgat aaaatcatat tcgaaacaaa tgataatgat caagaacaaa
    13441 acttagcttc tttagaagca catattcaag aagaagacga acaaagtgca cgattggcaa
    13501 cagagaaact tgaaaaaatg aaagctgaaa aagcgaaaca acaagataac aacgaaagtg
    13561 ctgtcgataa gtgtcaaatt ggtaagccga ttcaaattga aaatattaaa ccaattgaat
    13621 ctattattga ggaagagttt aaagttgcaa tagagggtgt catttttgat ataaacttaa
    13681 aagaacttaa aagtggtcgt catatcgtag aaattaaagt gactgactat acggactcat
    13741 tagttttaaa aatgtttact cgtaaaaaca aagatgattt agaacatttt aaagcgctaa
    13801 gtgtaggtaa atgggttagg gctcaaggtc gtattgaaga agatacattt attagagatt
    13861 tagttatgat gatgtctgat attgaagaga ttaaaaaagc gacaaaaaaa gataaggctg
    13921 aagaaaagcg tgtagaattc cacttgcata ctgcaatgag ccaaatggat ggtataccca
    13981 atattggtgc gtatgttaaa caggcagcag actggggaca tccagccatt gcggttacag
    14041 accataatgt tgtgcaagca tttccagatg ctcacgcagc agcggaaaaa catggcatta
    14101 aaatgatata cggtatggaa ggtatgttag ttgatgatgg tgttccgatt gcatacaaac
    14161 cacaagatgt cgtattaaaa gatgctactt atgttgtgtt cgacgttgag acaactggtt
    14221 tatcaaatca gtatgataaa atcatcgagc ttgcagctgt gaaagttcat aacggtgaaa
    14281 tcatcgataa gtttgaaagg tttagtaatc cgcatgaacg attatcggaa acgattatca
    14341 atttgacgca tattactgat gatatgttag tagatgcccc tgagattgaa gaagtactta
    14401 cagagtttaa agaatgggtt ggcgatgcga tattcgtagc gcataatgct tcgtttgata
    14461 tgggctttat cgatacggga tatgaacgtc ttgggtttgg accatcaacg aatggtgtta
    14521 tcgatacttt agaattatct cgtacgatta atactgaata tggtaaacat ggtttgaatt
    14581 tcttagctaa aaaatatggc gtagaattaa cgcaacatca ccgtgccatt tatgatacag
    14641 aagcaacagc ttacattttc ataaaaatgg ttcaacaaat gaaagaatta ggtgtattaa
    14701 atcataacga aatcaacaaa aaactcagta atgaagatgc atataaacgt gcaagaccta
    14761 gccatgtcac attaattgta caaaaccaac aaggtcttaa aaatctattt aaaattgtaa
    14821 gtgcatcatt ggtgaagtat ttctaccgta cacctcgaat tccacgttca ttgttagatg
    14881 aatatcgtga gggattattg gtaggtacag cgtgtgatga aggtgaatta tttacggcag
    14941 ttatgcagaa ggaccagagc caagttgaaa aaattgccaa atattatgat tttattgaaa
    15001 ttcaaccacc ggcactttat caagatttaa ttgatagaga gcttattaga gatactgaaa
    15061 cattacatga aatttatcaa cgtttaatac atgcaggtga cacagcgggt atacctgtta
    15121 ttgcgacagg aaatgcacac tatttgtttg aacatgatgg tatcgcacgt aaaattttaa
    15181 tagcatcaca acccggcaat ccacttaatc gctcaacttt accggaagca cattttagaa
    15241 ctacagatga aatgttaaac gagtttcatt ttttaggtga agaaaaagcg catgaaattg
    15301 ttgtgaaaaa tacaaacgaa ttagcagatc gaattgaacg tgttgttcct attaaagatg
    15361 aattatacac accgcgtatg gaaggtgcta acgaagaaat tagagaacta agttatacaa
    15421 atgcgcgtaa actgtatggt gaagacctgc ctcaaatcgt aattgatcga ttagaaaaag
    15481 aattaaaaag tattatcggt aatggatttg cggtaattta cttaatttcg caacgtttag
    15541 ttaaaaaatc attagatgat ggatacttag ttggttcccg tggttcagta ggttctagtt
    15601 ttgtagcgac aatgactgag attactgaag taaacccgtt accgccacac tatatttgtc
    15661 cgaactgtaa aacgagtgaa tttttcaatg atggttcagt aggatcagga ttcgatttac
    15721 ctgataagac gtgtgaaact tgtggagcgc cacttattaa agaaggacaa gatattccgt
    15781 ttgaaacatt tttaggattt aagggagata aagttcctga tatcgactta aactttagtg
    15841 gtgaatatca accgaatgcc cataactaca caaaagtatt atttggtgag gataaagtat
    15901 tccgtgcagg tacaattggt accgttgctg aaaagactgc ttttggttat gttaaaggtt
    15961 atttgaatga tcaaggcatc cacaaaagag gcgctgaaat agatcgactc gttaaaggat
    16021 gtacaggtgt taaacgtaca actggacagc atccaggagg tattattgta gtacctgatt
    16081 acatggatat ttatgatttt acgccgatac aatatcctgc cgatgatcaa aattcagcat
    16141 ggatgacgac acattttgat ttccattcta ttcatgataa tgtattaaaa cttgatatac
    16201 ttggacacga tgatccaaca atgattcgta tgcttcaaga tttatcgggc attgatccaa
    16261 aaacgatacc tgtagatgac aaagaagtca tgcaaatatt tagtacacct gaaagtttag
    16321 gtgttactga agatgaaatt ttatgtaaaa caggtacgtt tggggttcca gaattcggta
    16381 cagggttcgt gcgtcaaatg ttagaagata caaagccaac aacattttct gaattagttc
    16441 aaatctcagg attatctcat ggtacagatg tgtggttagg caatgctcaa gaattaatta
    16501 aaaccggtat atgtgattta tcaagtgtaa ttggttgtcg tgatgatatc atggtttatt
    16561 taatgtatgc tggtttagaa ccatcaatgg cttttaaaat aatggagtca gtacgtaaag
    16621 gtaaaggttt aactgaagaa atgattgaaa cgatgaaaga aaatgaagtg ccggattggt
    16681 atttagattc atgtcttaaa attaagtaca tgttccctaa agcccatgca gcagcatacg
    16741 ttttaatggc agtacgtatc gcatatttca aagtacatca tccactttat tactatgcat
    16801 cttactttac aattcgtgcg tcagattttg atttaatcac gatgattaaa gataaaacaa
    16861 gcattcgaaa tactgtaaaa gacatgtatt ctcgctatat ggatctaggt aaaaaagaaa
    16921 aagacgtatt aacagtcttg gaaattatga atgaaatggc gcatcgaggt tatcgaatgc
    16981 aaccgattag tttagaaaag agtcaggcgt tcgaatttat cattgaaggc gatacactta
    17041 ttccgccgtt catatcagtg cctgggcttg gcgaaaacgt tgcgaaacga attgttgaag
    17101 ctcgtgacga tggcccattt ttatcaaaag aagatttaaa caaaaaagct ggattatctc
    17161 agaaaattat tgagtattta gatgagttag gctcattacc gaatttacca gataaagctc
    17221 aactttcgat atttgatatg taaaatgaaa taatcaaggt atttatttaa tgcgtatggc
    17281 gtagttaaag aaatacaaaa ttggtgctgg acataaaatt atgcctgtat ttcttttcaa
    17341 cgtcttacga gtctattcga atgtaatggt gaaataaatg aacaaacttt tacaagaatc
    17401 tctgattaat agtgaagtca tttgtttcaa gcataaactt atgctataat taagttgctt
    17461 aaaaattagt gaactcaggc agaagagtgg gagattcccg ctcttttcta tttgccaaaa
    17521 agggaggcct gtatgagtaa aattacagaa caagtagaag tgattgttca accaattatg
    17581 gaagacttga attttgaact tgtagacgtt gaatatgtca aagagggtag agatcatttt
    17641 cttagaatct ctattgataa agaaggtggc gtagatttaa atgattgtac gctagcttct
    17701 gaaaaaataa gtgaagctat ggatgcaaat gatcctattc ctgaaatgta ttatttagac
    17761 gtagcgtcac ctggtgcaga acgtccaatt aaaaaagaac aagatttcca aaatgcaata
    17821 actaaacctg tatttgtttc tttatatgta ccaattgaag gtgaaaagga atggttaggc
    17881 attttacaag aagtcaataa tgaaacaatt gtagtacaag ttaaaatcaa agcaagaacg
    17941 aaagatatag agataccgag agacaaaata gcaaaagcac gtcacgcagt tatgatttaa
    18001 cgtgatgagg aggaaaaaac gtgtcaagta atgaattatt attagctact gagtatttag
    18061 aaaaagaaaa gaagattcct agagcagtat taattgatgc tattgaagca gctttaatta
    18121 ctgcatacaa aaagaattat gatagtgcaa gaaatgtccg tgtggaatta aatatggatc
    18181 aaggtacttt caaagttatc gctcgtaaag atgttgttga agaagtattt gacgacagag
    18241 atgaagtgga tttaagtaca gcgcttgtta aaaaccctgc atatgaaatt ggtgatatat
    18301 acgaagaaga tgtaacacct aaagattttg gtcgtgtagg tgctcaagca gcgaaacaag
    18361 cagtaatgca acgtcttcgt gatgctgaac gtgaaatttt atttgaagaa tttatagaca
    18421 aagaagaaga catacttact ggaattattg accgtgttga ccatcgttat gtatatgtga
    18481 atttaggtcg tatcgaagct gttttatctg aagcagaaag aagtcctaac gaaaaatata
    18541 ttcctaacga acgtatcaaa gtatatgtta acaaagtgga acaaacgaca aaaggtcctc
    18601 aaatctatgt ttctcgtagc catccaggtt tattaaaacg tttatttgaa caagaagttc
    18661 cagaaattta cgatggtact gtaattgtta aatcagtagc acgtgaagct ggcgatcgct
    18721 ctaaaattag tgtcttctct gaaaacaatg atatagatgc tgttggtgca tgtgttggtg
    18781 ctaaaggcgc acgtgttgaa gctgttgttg aagagctagg tggcgaaaaa atcgacatcg
    18841 ttcaatggaa tgaagatcca aaagtatttg taaaaaatgc tttaagccct tctcaagttt
    18901 tagaagttat tgttgatgaa acaaatcaat ctacagtagt tgttgttcct gattatcaat
    18961 tgtcattagc gattggtaaa agaggacaaa acgcacgtct agctgctaaa ttaaccggct
    19021 ggaaaattga tattaaatca gaaacagatg cgcgtgaagc gggtatctat ccagtagttg
    19081 aagctgaaaa agtaactgaa gaagatattg ctttagaaga tgctgacaca acagaatcaa
    19141 ccgaagaggt aaatgatgtt tcagttgaaa caaatgtaga gaaagaatct gaataatagg
    19201 ttggagtgaa gtatctatga aaaagaaaaa aattccgatg cgaaaatgta ttctttcaaa
    19261 tgaaatgcat cccaaaaaag atatgattcg tgttgttgtt aataaagaag gcgaaatctt
    19321 tgcggatgtt actggaaaga aacaaggccg tggcgcatat gtttctaaag atgttgctat
    19381 ggttgaaaaa gcacaacaaa aagaaatttt agaaaaatat tttaaagcat ctaaagagca
    19441 attggatcct gtttacaaag aaattattag attaatttat agagaagaga ttccaaaatg
    19501 agtatagatc aaatattaaa ctttttagga ttagcaatga gagctggtaa agtaaaaaca
    19561 ggtgaatcag tcattgttaa tgagattaaa aaaggaaatt tgaagctcgt tattgttgca
    19621 aatgatgcgt ctgataatac agctaaatta attacagata aatgtaagag ttacaaagtt
    19681 ccattcagaa agtttggaaa tcgaaatgaa ttgggaatag cacttggaaa aggtgagcgt
    19741 gttaatgtag ggattactga cccaggcttt gctaaaaagt tgctatcaat gatagatgaa
    19801 tatcataagg agtgattata tgagtaaaca aagaatttac gaatatgcga aagaattaaa
    19861 tctaaagagt aaagagatta tagatgagtt aaaaagcatg aatatagagg tttcaaatca
    19921 tatgcaagct ttggaagatg accaaattaa agcattagat aaaaagttca aaaaagaaca
    19981 aaagaacgac aataaacaaa gcactcaaaa taatcaccaa aaatcaaaca atcaaaacca
    20041 aaataaaggg caacaaaaag ataacaaaaa gaatcaacaa caaaataata aaggcaacaa
    20101 aggcaataaa aagaataata gaaataataa gaaaaataac aagaataata aaccacaaaa
    20161 tcaaccagct gctccaaaag aaataccatc aaaagtgaca tatcaagaag gtattacagt
    20221 aggcgaattt gcggataaat taaatgttga atcatcagaa attatcaaaa aattattctt
    20281 acttggtatt gttgctaata tcaatcaatc attaaatcaa gaaacaatcg aattaattgc
    20341 cgatgattat ggcgttgagg ttgaagaaga agttgtgatt aatgaagaag atttatcaat
    20401 ctacttcgaa gacgaaaaag atgatccaga agcaattgag agaccagcag ttgtaacaat
    20461 tatgggacat gttgaccatg gtaaaacgac tttattagat tcaattcgtc atacaaaagt
    20521 tacagcaggt gaagcaggcg gaatcactca acatattggt gcatatcaaa ttgaaaacga
    20581 tggcaaaaaa atcactttct tagatacacc gggacatgct gcatttacaa cgatgcgtgc
    20641 gcgtggtgca caagtaacag atattacaat tttagtagta gcagctgacg atggtgttat
    20701 gccacaaaca attgaagcaa ttaaccatgc taaagaagca gaagtaccaa ttattgttgc
    20761 agtaaataaa attgataaac caacttcaaa tcctgatcga gttatgcaag aattaactga
    20821 atatggttta attcctgaag attggggcgg cgaaacaatt ttcgttccac tttctgcatt
    20881 aagtggtgat ggtatcgacg atttattaga aatgatagga ttagttgcag aagttcaaga
    20941 acttaaagca aatcctaaaa accgtgctgt tggtacagtt atcgaagctg aattagataa
    21001 atcacgtggt ccttctgcat cattattagt acaaaacggt acattaaatg ttggtgatgc
    21061 gattgtagtt ggtaatactt acggccgtat ccgtgcaatg gttaatgact taggtcaaag
    21121 aatcaaaacg gctggtccat caacgcctgt tgaaattaca ggtattaatg atgtgccaca
    21181 agctggggat cgctttgttg tatttagtga tgaaaaacaa gctcgtcgta ttggtgaatc
    21241 aagacacgaa gctagcattg tacaacaacg tcaagaaagt aaaaatgttt cattagataa
    21301 cctgtttgaa caaatgaaac aaggtgaaat gaaagattta aacgttatta ttaaaggtga
    21361 tgttcaaggt tctgttgaag ctttagctgc atcattaatg aaaattgatg ttgaaggcgt
    21421 aaatgttcgt atcattcata cagcggttgg tgcaattaat gagtcagacg tgacacttgc
    21481 taatgcctca aatggtatta tcattggttt caatgttcgt ccagacagtg gtgcaaaacg
    21541 tgctgcagaa gctgaaaatg ttgatatgcg tttacacaga gttatttata atgttatcga
    21601 agaaattgaa tcagcgatga aaggtttact tgatccagaa tttgaagaac aagttatcgg
    21661 acaagctgaa gttcgtcaaa cattcaaagt ttctaaagtt ggtactattg ctggatgtta
    21721 tgttactgaa ggtaaaatta cgcgaaatgc tggtgtacgt attattcgtg atggtattgt
    21781 tcaatatgaa ggcgaattag atacacttaa acgtttcaaa gatgatgcta aggaagttgc
    21841 aaaaggttat gaatgtggta ttacaattga aaactacaat gaccttaaag aaggcgatgt
    21901 tatcgaagca tttgaaatgg ttgaaattaa gcgttaatta aataaattac aagctaaaag
    21961 tatagttaag attgatatgc tccctataaa tattgcactt tttaagtgtc tactttatag
    22021 ggagcatatt tgatactagc ttttggtttt ttattagaat agattaccta ttaaaagtta
    22081 cgttatatgg acatgatttt gtataaaatt ttgtggtggc ctagaatgat ttttaatgac
    22141 aaaatataat gtcgactatt attggaaaat tttctgttga aatgcctatc ttacggcaaa
    22201 ctttatttga ttttataggc ttaatttatt aaaataacgt gtgagctaaa ataattgttt
    22261 aagcattgtt acactaaaaa atgcaaataa caattgaact taaagataaa gaggtgacaa
    22321 gaatgagcag tatgagagca gagcgtgttg gtgaacaaat gaagaaggaa ttaatggata
    22381 tcatcaacaa taaagtcaaa gatcctcgag ttggttttat tacaattaca gatgttgttt
    22441 taacaaatga tttatcgcag gctaaagtat ttttaactgt attaggtaac gataaagaag
    22501 tagaaaatac atttaaagca cttgataaag caaaaggctt cattaagtct gaattaggtt
    22561 ctagaatgcg attacgtatt atgccggaat taatgtatga atatgatcaa tcaatcgaat
    22621 atggtaataa aattgaacga atgattcaag atttacacaa acaagataga taatttagtg
    22681 ttaggtatct ggaaaatgtt tgataatttc ttaatatcgg tatattaaca ttaaacagtt
    22741 aatacataga tgtgtagaaa tagttaacat tttccagttt ttttatgaat aaattcagtt
    22801 gatacgctat taaaatatat tttaaaaaag aaggtgacta tatgtataat gggatattac
    22861 cagtatataa agagcgcggt ttaacaagtc atgacgttgt attcaaattg cgtaaaatat
    22921 taaaaactaa aaaaataggt cacacgggta cgcttgatcc cgaagttgca ggcgtgttac
    22981 cggtatgtat aggtaatgca acgagagtta gtgattatgt tatggatatg ggcaaagctt
    23041 atgaagcaac tgtatcgata ggaagaagta caacgactga agatcaaacg ggtgatacat
    23101 tggaaacaaa aggtgtacac tcagcagatt ttaataagga cgatattgac cgattgttag
    23161 aaaattttaa aggtgttatt gaacaaattc cgccaatgta ttcatcagtc aaggtgaatg
    23221 gcaaaaaatt atatgaatat gcgcgtaata atgaaacagt tgaaagacca aagcgtaaag
    23281 ttaatattaa agacattggg cgtatatctg aattagattt taaagaaaat gagtgtcatt
    23341 ttaaaatacg cgtcatctgt ggtaaaggta catatattag aacgctagca actgatattg
    23401 gtgtgaaatt aggctttccg gcacatatgt cgaaattaac acgaatcgag tctggtggat
    23461 ttgtgttgaa agatagcctt acattagaac aaataaaaga acttcatgag caggattcat
    23521 tgcaaaataa attgtttcct ttagaatatg gattaaaggg tttgccaagc attaaaatta
    23581 aagattcgca cataaaaaaa cgtattttaa atgggcagaa atttaataaa aatgaatttg
    23641 ataacaaaat taaagaccaa attgtattta ttgatgatga ttcagaaaaa gtattagcaa
    23701 tttatatggt acaccctaca aaagaatcag aaattaaacc taaaaaagtc tttaattaaa
    23761 ggagatagaa tttatgaaag tcatagaagt gacacatcct atacaatcta aacagtatat
    23821 tacagaggat gttgcaatgg cattcggatt tttcgatggc atgcataaag gtcatgacaa
    23881 agtctttgat atattaaatg aaatagctga ggcacgcagt ttaaaaaaag cggtgatgac
    23941 atttgatccg catccgtctg tcgtgttgaa tcctaaaaga aaacgaacaa cgtatttaac
    24001 accactttca gataaaatcg aaaaaattag ccaacatgat attgattatt gtatagtggt
    24061 taatttttca tctaggtttg ctaatgtgag cgttg
//
LOCUS       DANBWH010000014        23642 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.014, whole genome shotgun sequence.
ACCESSION   DANBWH010000014 DANBWH010000000
VERSION     DANBWH010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 23642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23642
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.014"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..732)
                     /gene="ccsA"
                     /locus_tag="PPY92_000398"
     CDS             complement(<1..732)
                     /gene="ccsA"
                     /locus_tag="PPY92_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HDJ2624422.1"
                     /translation="MQENLFIRFNEIILLIYLISIICYFYDFVQKSHKIRSLGIYLLG
                     IVWVLQTISLSIFIIQTRHIPLGSISDVFYTLSWLIISISLILNLIKVLNFSVFFLNL
                     IGLTLLGMNTFQPTHYTNKVQKIAVVDELLLVHIGLAVLSYVFFALAFVNALLYIMQY
                     RNLKEKRFDQKYFRIGSVATLESIVFYSTLSGWIILIFSIILGTQWGVISVGERIFID
                     PKVILSSIITVLYGSYILLRINKWLN"
     gene            complement(754..2100)
                     /locus_tag="PPY92_000399"
     CDS             complement(754..2100)
                     /locus_tag="PPY92_000399"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDJ2624423.1"
                     /translation="MHFIAISINHRTADVALREQVAFRDDALRIAHEDLYETKSILEN
                     VILSTCNRTEVYAVVDQIHTGRYYIQRFLARAFGFEVDDIKAMSEVKVGDEAVEHLLR
                     VTSGLDSIVLGETQILGQIRDAFFLAQSTGTTGTIFNHLFKQAITFAKRAHNETDIAD
                     NAVSVSYAAVELAKKVFGKLKSKQAIIIGAGEMSELSLLNLLGSGITDITVVNRTIEN
                     AMKLAAKHQVKYDELSSLPNLLESADIVISSTSAQSYIITNEMIERIAENRKQDSLVL
                     IDIAVPRDIEPGISAITNIFNYDVDDLKGLVDANLRERQLAAATISEQIPAEIHAHNE
                     WISMLGVVPVIRALREKAMAIQAETMDSIDRKLPGLSERERKIISKHTKSIINQMLKD
                     PIKQAKELSSDKKSNEKLELFQNIFDIEAECPHEQAKQQKESKVKEISARRIFSFE"
     gene            complement(2317..2907)
                     /locus_tag="PPY92_000400"
     CDS             complement(2317..2907)
                     /locus_tag="PPY92_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDJ2624424.1"
                     /translation="MKVNPNNIELIISAVKEEQYPETELSEVALSGRSNVGKSTFINS
                     MIGRKNMARTSQQPGKTQTLNFYNIDEQLIFVDVPGYGYAKVSKTQREKFGKMIEEYI
                     TKRENLQLVIQLVDLRHDPTQDDILMYNYLKHFDIPTLVICTKEDKIPKGKVQKHIKN
                     IKTQLDMDPDDTIVSYSSIQNNKQQQIWNLIEPYIS"
     gene            complement(3061..4323)
                     /gene="clpX"
                     /locus_tag="PPY92_000401"
     CDS             complement(3061..4323)
                     /gene="clpX"
                     /locus_tag="PPY92_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDJ2624425.1"
                     /translation="MFKFNEDEENLKCSFCGKDQDQVKKLVAGSGVYICNECIELCSE
                     IVEEELAQNTSEAMTELPTPKEIMDHLNEYVIGQEKAKKSLAVAVYNHYKRIQQLGPK
                     EDDVELQKSNIALIGPTGSGKTLLAQTLAKTLNVPFAIADATSLTEAGYVGDDVENIL
                     LRLIQAADFDIDKAEKGIIYVDEIDKIARKSENTSITRDVSGEGVQQALLKILEGTTA
                     SVPPQGGRKHPNQEMIQIDTTNILFILGGAFDGIEEVIKRRLGEKVIGFSSNEADKYD
                     EQALLAQIRPEDLQAYGLIPEFIGRVPIVANLETLDVTALKNILTQPKNALVKQYTKM
                     LELDDVDLEFTEEALSAISEKAIERKTGARGLRSIIEESLIDIMFDVPSNENVTKVVI
                     TAQTINEETEPELYDAEGNLINNSKTSA"
     gene            complement(4474..5775)
                     /locus_tag="PPY92_000402"
     CDS             complement(4474..5775)
                     /locus_tag="PPY92_000402"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDJ2624426.1"
                     /translation="MTATWEKKEGNEGLLTVTVPAEKVNKALDQAFKKVVKQINVPGF
                     RKGKVPRPIFEQRFGVEALYQDAIDILLPDAYGEAIDETDIKPVAQPEVSVTQIEKGK
                     DFIFEATVTVEPEVKLGDYKGLEIEKQETELSDDELQEAIDHSLGHLAEMVVKEDGVV
                     ENGDTVNIDFSGSVDGEEFEGGQAEGYDLEIGSGSFIPGFEEQLEGMKVDEEKDVVVT
                     FPEEYHAEELAGKEATFKTKVNEIKFKEVPELTDEIANELDAEANTVDEYKENLRKRL
                     AEQKATDAENVEKEEAITKATDNTTIDIPEAMVNTELDRMVSEFAQRIQQQGLDLQTY
                     FQISGQDETQLREQMKDDAEQRVKTNLTLTAIAEAEKIEATDEDIDKELEKMSKQFNI
                     SVEDIKNTLGNTDIIKNDVRIQKVIDLLRDNAKFVEGTKED"
     gene            complement(5823..5933)
                     /locus_tag="PPY92_000403"
     CDS             complement(5823..5933)
                     /locus_tag="PPY92_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624427.1"
                     /translation="MQNNTQHFNIVLTKKVDLQIYKRCDFNVCIGNRPMI"
     gene            complement(5938..6867)
                     /locus_tag="PPY92_000404"
     CDS             complement(5938..6867)
                     /locus_tag="PPY92_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624428.1"
                     /translation="MSDIIQFPNSSKKLYKDIKRAEQDQNYDLMYEYIVQYERQFELT
                     EEIAMMKCRMLYETGSFLELREETIVLLKTGIQQYDALMIYYVKSLIGLGQYFEAVEV
                     IHQIIDEVKDHKTRMALHPLKEFAKSKLIEDEKRLTQSLTDFDTLSMREQTHLILKLI
                     DNGHFQFQETVLYILKSNTYSYNLISLMIEYLRFANCTQELTIEKYGMDVTFVPANLN
                     GLEHTTLKEKVIPNVMETLNDGALHIAEEAHHIMNNHSIMIYPIDIETLFETNKWINA
                     YECYFKNMLGLKCELQSIDAFNFIQQLDLNNNS"
     gene            complement(6886..7494)
                     /locus_tag="PPY92_000405"
     CDS             complement(6886..7494)
                     /locus_tag="PPY92_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDJ2624429.1"
                     /translation="MSKFDEQIIVVPREIIFNNEKNTFNGFLNKNKPEGQNIFDALSQ
                     YEVKRRGDMEEDPSYKQLISYCLLENEHGEILVYERLSGGGEARLHGQSSIGVGGHMN
                     DVPGAESINEVLRVNAQRELEEEVGLSEQDSQNMEYIGFINDDNNEVGKVHIGVVFKI
                     TVSTNDVEAKETDTLRIKWVEKGNIESYDDFETWSALILQDL"
     gene            complement(7636..7992)
                     /gene="rplT"
                     /locus_tag="PPY92_000406"
     CDS             complement(7636..7992)
                     /gene="rplT"
                     /locus_tag="PPY92_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDJ2624430.1"
                     /translation="MPRVKGGTVTRARRKKTIKLAKGYFGSKHTLYKVAKQQVMKSGQ
                     YAFRDRRQRKRDFRKLWITRINAAARQHEMSYSRLMNGLKKAGIDINRKMLSEIAISD
                     EKAFAQLVTKAKDALK"
     gene            complement(8039..8239)
                     /gene="rpmI"
                     /locus_tag="PPY92_000407"
     CDS             complement(8039..8239)
                     /gene="rpmI"
                     /locus_tag="PPY92_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDJ2624431.1"
                     /translation="MPKMKTHRGAAKRVKRTASGQLKRSRAFTSHLFANKSTKQKRQL
                     RKARLVSKSDMKRVKQLLAYKK"
     gene            complement(8268..8795)
                     /gene="infC"
                     /locus_tag="PPY92_000408"
     CDS             complement(8268..8795)
                     /gene="infC"
                     /locus_tag="PPY92_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDJ2624432.1"
                     /translation="MSTIAKDQTQINDKIRAKELRLIGQDGEQIGVKSKREALEMAER
                     VDLDLVVVAPNAKPPVARIMDYGKFKFEQQKKEKEMKKKQKIINVKEIRLSPTIEEHD
                     FQTKLKNGRKFLTKGDKCKVSIRFRGRAITHKEIGQRVLEKYADECKDIATVEQKPKM
                     DGRQMFIMLAPTAEK"
     misc_feature    complement(8824..8949)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            complement(9024..10517)
                     /locus_tag="PPY92_000409"
     CDS             complement(9024..10517)
                     /locus_tag="PPY92_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDJ2624433.1"
                     /translation="MSKVQNESNNVVKRGLKDRHISMIAIGGCIGTGLFVTSGGAIHD
                     AGALGALIGYAIIGIMVFFLMTSLGEMATYLPVSGSFSTYATRFVDPSLGFALGWNYW
                     FNWVVTVAADITIAAQVIQYWTPLQGIPAWAWSALFLVIIFSLNSLSVRVYGESEYWL
                     ALIKVITVIVFIAIGLLTIVGIMGGHVVGFEIFNKGEGPILGGNLGGSLLSILGVFLI
                     AGFSFQGTELIGITAGESENPERAVPKAIKQVFWRILLFYILAIFVIGMLIPYDSSAL
                     MGGSDNVATSPFTLVFKNAGFAFAASFMNAVILTSVLSAGNSGMYASTRMLYSMSKDK
                     LAFEAFGKTNKNGVPYMSLLVTAIIVVIIFVLQSLTKGAYEYIVAASGLTGFIAWVGI
                     AVSHYRFRRAFDKQNYDKSKLKYTAKLFPFGPLFAGFLCIVVIIGQDVDFIKTGDFNF
                     SRFLITYMGIPVFLAFFIYHKLRYKTKKIPLEQVDLRQDVSMDEIKG"
     gene            complement(10943..12880)
                     /gene="thrS"
                     /locus_tag="PPY92_000410"
     CDS             complement(10943..12880)
                     /gene="thrS"
                     /locus_tag="PPY92_000410"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDJ2624434.1"
                     /translation="MEQINIQFPDGNKKAFDKGTTTEDIAQSISPGLRKKAVAGKFNG
                     QLVDLTKPLETDGSIGIVTPGSEEALEVLRHSTAHLMAHAIKRLYGNVKFGVGPVIEG
                     GFYYDFDIDQNISSDDFEQIEKTMKQIVNENMKIERKVVSRDEAKELFSNDEYKLELI
                     DAIPEDENVTLYSQGDFTDLCRGVHVPSTAKIKEFKLLSTAGAYWRGDSNNKMLQRIY
                     GTAFFDKKELKAHLQMLEERKERDHRKIGKELELFTNSQLVGAGLPLWLPNGATIRRE
                     IERYIVDKEVSMGYDHVYTPVLANVDLYKTSGHWDHYQEDMFPPMQLDETESMVLRPM
                     NCPHHMMIYANKPHSYRELPIRIAELGTMHRYEASGAVSGLQRVRGMTLNDSHIFVRP
                     DQIKEEFKRVVNMIIDVYKDFGFEDYSFRLSYRDPEDKEKYFDDDDMWNKAENMLKEA
                     ADELGLSYEEAIGEAAFYGPKLDVQVKTAMGKEETLSTAQLDFLLPERFDLTYIGQDG
                     EHHRPVVIHRGVVSTMERFVAFLTEETKGAFPTWLAPKQVQIIPVNVDLHYDYARQLQ
                     DELKSQGVRVSIDDRNEKMGYKIREAQMQKIPYQIVVGDKEVENNQVNVRQYGSQDQE
                     TVEKDEFIWNLVDEIRLKKHR"
     gene            complement(13078..13200)
                     /locus_tag="PPY92_000411"
     CDS             complement(13078..13200)
                     /locus_tag="PPY92_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624435.1"
                     /translation="MFDENMVNKLDDYYFIYGICNEYPDQDRYLKQRYLIHKLY"
     gene            complement(13293..14213)
                     /gene="dnaI"
                     /locus_tag="PPY92_000412"
     CDS             complement(13293..14213)
                     /gene="dnaI"
                     /locus_tag="PPY92_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HDJ2624436.1"
                     /translation="MKQFKSIINTSQDFEKRIEKIKKEVINDPDVKQFLEAHRAELTN
                     AMIDEDLNVLQEYKDQQKHYDGHKFADCPNFVKGHVPELYVDNNRIKIRYLQCPCKIK
                     YDEERFEAELITSHHMQRDTLNAKLKDIYMNHRDRLDVAMAADDICTAITNGEQVKGL
                     YLYGPFGTGKSFILGAIANQLKSKKVRSTIIYLPEFIRTLKGGFKDGSFEKKLHRVRE
                     ANILMLDDIGAEEVTPWVRDEVIGPLLHYRMVHELPTFFSSNFDYSELEHHLAMTRDG
                     EEKTKAARIIERVKSLSTPYFLSGENFRNN"
     gene            complement(14213..15613)
                     /locus_tag="PPY92_000413"
     CDS             complement(14213..15613)
                     /locus_tag="PPY92_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HDJ2624437.1"
                     /translation="MGRQAFEFGLRPKDQFKVMQHFDLNTNHLEVLNRLYTPLIGTQA
                     VGLYHFMTQFVKDSHNETLILSHYIFMNELKINLLEFRQQMDLLEAIGLLKAFVKHDE
                     QETQFVYQLIQPPSAHLFFNDPMLSIFLYSEVEHRRFHELKKYFEYQQIDLSEFKQVT
                     RQFTDVFKVPSTKIDIDTSDIPINEPYQGIDLSNESFDFEMLRQMLGKHFISQDIVTK
                     DAKRLITQLATLYGLTADGMKHVILNSITSGQQLSFEEMRKQARSYYLMEHENQMPKL
                     QVKSPATSSSTGKSSEVNPKPQSDEWFELLEQTSPIDMLASWSESEPTISQKTMVEEL
                     IEREKMSFGVINILLQFVMLKEDMKLPKAYILEIASNWKKKGIKTAKEAYNYAKKVNQ
                     PKNEGSSGNYQKRGSYYGQRNRISKEKTPKWLENRDKPSEEDSAKDNSVDDQQLEQDR
                     QAFLDKLSKKWEEDSR"
     gene            complement(15614..16084)
                     /gene="nrdR"
                     /locus_tag="PPY92_000414"
     CDS             complement(15614..16084)
                     /gene="nrdR"
                     /locus_tag="PPY92_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDJ2624438.1"
                     /translation="MKCPKCNSTQSKVVDSRHADELNAIRRRRECENCGTRFTTFEHI
                     EVSQLIVVKKDGTREQFSREKILNGLVRSCEKRPVRYQQLEDITNKVEWQLRDEGHTE
                     VSSRDIGEHVMNLLMHVDQVSYVRFASVYKEFKDVDQLLASMQGILSENKRSDA"
     gene            complement(16295..17320)
                     /gene="gap"
                     /locus_tag="PPY92_000415"
     CDS             complement(16295..17320)
                     /gene="gap"
                     /locus_tag="PPY92_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDJ2624439.1"
                     /translation="MSTNIAINGMGRIGRMVLRIALQNKNLNVVAINASYPPETIAHL
                     INYDTTHGKYNLKVEPIENGLQVGDHKIKLVADRNPENLPWKELDIDIAIDATGKFNH
                     GDKAIAHIKAGAKKVLLTGPSKGGHVQMVVKGVNDNQLDIEAFDIFSNASCTTNCIGP
                     VAKVLNNQFGIVNGLMTTVHAITNDQKNIDNPHKDLRRARSCNESIIPTSTGAAKALK
                     EVLPELEGKLHGMALRVPTKNVSLVDLVVDLEKEVTAEEVNQAFENAGLEGIIEVEHQ
                     PLVSVDFNTNPNSAIIDAKSTMVMSGNKVKVIAWYDNEWGYSNRVVDVAEQIGALLTS
                     KETVSAS"
     gene            complement(17490..18113)
                     /locus_tag="PPY92_000416"
     CDS             complement(17490..18113)
                     /locus_tag="PPY92_000416"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDJ2624440.1"
                     /translation="MPKVIGLTGGIASGKSTVSELLSVFGFKVVDADKAAREAVKKGS
                     KGLAQVREVFGDEAIDENGEMNRRYMGDLVFNHPEKRLELNAIIHPIVRDIMEEEKQE
                     YLKQGYNVIMDIPLLFENELENTVDEVWVVYTSESIQMDRLMQRNNLSLEDAKARVYS
                     QISIDKKSRMADHVIDNLGDKLELKQNLERLLEEEGYIEKPNYGEED"
     gene            complement(18129..19001)
                     /gene="mutM"
                     /locus_tag="PPY92_000417"
     CDS             complement(18129..19001)
                     /gene="mutM"
                     /locus_tag="PPY92_000417"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDJ2624441.1"
                     /translation="MPELPEVEHVKRGIEPYVINQKIEHVIFSDKVIEGKAQGKETII
                     KGIELDTFKTLSEGYTITNVERRSKYIVFQLDNKREQRTLISHLGMAGGFFIVDELED
                     IMIPNYRKHWHVIFELSNDKKLIYSDIRRFGEIRNVASVASYPSFLEIAPEPFTNEAL
                     TYYLNRIHQQSNKNKPIKQVILDHKVIAGCGNIYACEALFRAGVLPDKKVKDLTHQQQ
                     EMVFYYVREVLEEGIKHGGTSISDYRHADGKTGEMQLHLNVYKQPVCKVCGSQIETKI
                     IATRNSHYCPVCQK"
     gene            complement(19017..21647)
                     /gene="polA"
                     /locus_tag="PPY92_000418"
     CDS             complement(19017..21647)
                     /gene="polA"
                     /locus_tag="PPY92_000418"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDJ2624442.1"
                     /translation="MNKLVLIDGNSLSFRAFYALPLLSNKAGIHTNAVYGFAMLLEKI
                     LKEEKPNHFLVAFDAGKTTFRHEKYSEYKGGRQKTPPELSEQFPYIRQLLDAYHIKRY
                     ELDNYEADDIIGTLSKEADKAGFQTIIITGDRDLTQLATDNVTIYYTKKGVTDVDHYT
                     PDFIAEKYNGLTPNQIIDMKGLMGDTSDNIPGVAGVGEKTAIKLLNQFDTVEGVYEHL
                     DEISGKKLKEKLQNSKEDALMSKELATINVDSPIEVKLEDTLMTHQDEQQEKIELFKK
                     LEFKQLLSDIDQSASVEDAIEKTFEIETSFDNIDFTSLKEAAIHFELDGGNYLRNNIL
                     KFSLFTGEKHIVINADDINNYAELVSWLENPNTKKVVYDAKKTYVASHRLGIDIQNIS
                     FDIMLASYIIDPSRTISDVQSVVSLYGQSFVKDDVSIYGKGKKFKVPEDDVLNPYVAS
                     ITDAIYFAKPNMDKQLEEYNQVELLADLELPLAKILSEMEEIGIFTDVHDLEEMEKEI
                     QEKLDVLIRNIHDAAGEDFNINSPKQLGVVLFETLQLPVIKKTKTGYSTAVDVLEQLQ
                     GEHPIIDYILEYRQLSKLQSTYVEGLQKVISDDQRIHTRFNQTLAQTGRLSSVDPNLQ
                     NIPVRLEEGRKIRKAFKPTSKDSVILSADYSQIELRVLAHITQDESMKEAFINGDDIH
                     TATAMKVFGVEADQVDSLMRRQAKAVNFGIVYGISDYGLSQSLGITRKKAKAFIDDYL
                     ASFPGVKQYMSDIVKDAKALGYVETLLHRRRYIPDITSRNFNLRGFAERTAMNTPIQG
                     SAADIIKLAMVKFAQKMKETTYQAKLLLQVHDELIFEVPKSEVDSFSEFVEEIMENAL
                     QLDVPLKVDSSYGATWYDAK"
     gene            complement(21941..23428)
                     /locus_tag="PPY92_000419"
     CDS             complement(21941..23428)
                     /locus_tag="PPY92_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624443.1"
                     /translation="MKTIQLHKTTLILIAILLFYTFMGILLPIMHDDLQWFSNYNTDI
                     LKVGFASLNGRYIGNIFEIIAVHVSWLRWLSYGLISMGIIWMIMHITRCKAWTSYYLL
                     AFSLMLILPSAIYADTYGWFAGFYNYATSTLISLFIIYYCINAIIYKEKQPVSVTVLF
                     YVLCFFGQLFMENVTLFHCIILILAFLYEFTANRTLNYKLLFSFMIATIGTIIMFSNP
                     NYRKILFEGSEYQQVSNNQGIFSKFAEMISTSLPYGVIFSQIIILSMIAALIIYLLLR
                     SDRYVHLTILKRRIIMIGFITLPLYYLLFYNQFLLNKNTDIGLVGFVNVIVCGYFAFS
                     IFVGIYLSINDRKTQITLYSLLIAICVSASTLVIVTPIEPGNFLIVYTIHVIILIILL
                     KEFRKYKSINVNFIKGASLVLAVIYLSAFIYVHYEHEKRVQLLKQQIKDHPRQEVYTV
                     ESLPFEHYMHHPSPINKNYQEWFNNYEELPKMTKVKYIPFGSDDS"
BASE COUNT         7353 a         4448 c         3175 g         8666 t
ORIGIN      
        1 attcaaccat ttgttgattc taagcaatat ataacttcca tataatactg taataattga
       61 agagagtatt accttcgggt caatgaatat acgttcacca actgaaatta caccccactg
      121 tgtacctaaa ataatactaa atatgagaat tatccaccca cttaacgttg agtaaaacac
      181 aattgattca agtgtagcaa cgctacctat tctaaagtat ttttgatcaa aacgtttttc
      241 cttcaaatta cggtattgca tgatatacag taatgcattg acaaaagcta aggcaaagaa
      301 gacataactt aacacagcta gaccgatatg gactaacagt aactcgtcta caacagcaat
      361 tttctgaacc ttattagtat aatgtgtcgg ttgaaatgta ttcatcccta aaagtgttaa
      421 ccctattaaa ttcaagaaaa acacagagaa attcaatact ttgataaggt taagaattaa
      481 agagattgaa ataatcagcc aacttaaagt atagaataca tctgaaatag accctaatgg
      541 aatatgtcta gtttgtataa taaaaataga taaagagatt gtttgtaaaa cccaaacaat
      601 ccccaataaa tatatgccta aacttctaat cttatgactt ttttggacaa aatcataaaa
      661 atagcaaatg atactgatta agtatattaa taatataatt tcattgaatc gaataaacag
      721 gttttcttgc atattatcac catattaata tgcttattca aaactaaaga tacgtcgcgc
      781 tgaaatttcc ttgaccttgc tttctttttg ttgcttcgct tgctcatgag gacattcagc
      841 ttcgatatca aatatatttt gaaatagctc taatttttca ttacttttct tatcactact
      901 taattctttg gcctgtttaa taggatcttt caacatttga ttgatgatac tttttgtatg
      961 tttagatata attttacgtt ctctttcgct taaccctggc aatttacgat caatactgtc
     1021 catcgtttct gcttgaattg ccatagcttt ttcacgtaaa gctctaatca ctggaacaac
     1081 acccaacata ctaatccact cattgtgtgc atgtatttct gcaggaattt gttccgaaat
     1141 tgttgcagcc gctaattgtc gctcacgtaa gtttgcatca actaaacctt ttaagtcatc
     1201 aacatcataa ttaaagatgt ttgtgatggc actaatacca ggttcaatat ctcgaggaac
     1261 tgcaatatca atcaatacta gtgaatcttg ctttctattt tctgcaattc tttctatcat
     1321 ttcatttgta atgatataag attgtgcact cgttgaacta atcacaatat ctgcactttc
     1381 aagtaaattt ggtaatgatg atagttcatc atatttcact tgatgctttg ctgctaattt
     1441 catagcattt tcaattgttc tatttactac tgtaatatca gtaattccag aaccaagaag
     1501 atttaatagt gataattcac tcatttcccc tgcaccaata atgatagctt gcttactttt
     1561 caatttgcca aatacttttt tcgccaactc gaccgcagca taagacacac ttacagcatt
     1621 atcagctata tctgtttcat tatgtgctct ttttgcaaaa gtaattgcct gtttaaatag
     1681 atgattaaaa attgttcctg tcgtacctgt gctttgcgct aagaaaaatg catctcttat
     1741 ttgacctaaa atttgagttt ctccaagtac gattgaatct aaaccagaag tgacacgcaa
     1801 taaatgttct actgcttcgt cccccacttt tacttctgac attgctttaa tatcatctac
     1861 ttcaaatcca aatgcacgag ctaaaaatcg ttgaatatag taacgacctg tgtgaatttg
     1921 atcaacaaca gcatatactt cagttcgatt acatgttgat aatatgacat tttctaaaat
     1981 agatttagtt tcatataaat cttcatgggc aattcgtaag gcatcatctc taaaagcaac
     2041 ttgctctctt agtgcaacat cagctgtgcg atgatttata ctaattgcaa taaaatgcat
     2101 ttgaaacgcc cccatatata ctaacaatac aaatattata acataattta aaatccaatt
     2161 aagtacgatg cttaaataaa ttataatctt tctaatttac ttttgagttt ttgacaaaat
     2221 gatgaacgtt gaaaaatgtt atcaattgtg tactaattaa aagtttcgtg cttacattat
     2281 ttttaaaatt tttgtataag ttttgtcgta caaaaactat gaaatatacg gttcaattaa
     2341 attccatatt tgttgttgtt tattattttg aattgatgaa taacttacaa ttgtatcgtc
     2401 tgggtccata tctaattgtg tcttaatatt tttaatatgc ttttgaactt taccttttgg
     2461 aattttgtct tctttagtgc atataactaa agtaggaata tcaaaatgtt tcaaataatt
     2521 gtacattaag atatcatctt gtgttggatc atgtcttaaa tcaactaatt gaataactaa
     2581 ttgcaaattc tctctcttag ttatatattc ctcaatcatt ttcccaaatt tttcacgttg
     2641 tgttttactt actttagcat atccataccc tggaacatcc acaaaaataa gttgttcatc
     2701 tatattataa aaatttaacg tttgcgtttt gccgggttgc tgtgatgtac gtgccatatt
     2761 ttttctgcca atcatactat tgataaatgt agacttacct acattagatc gaccgctcag
     2821 tgcaacttca gacaattctg tttctggata ttgttcttct tttactgcac taatgattaa
     2881 ttcaatatta ttaggattaa ctttcatttt atatcctcac ttttttatat tctctttcaa
     2941 ctctaccatt atataatatt tgaacctttc ggtatacatt ataaaaaagc tccgatcaaa
     3001 gttaaactat ttgagcgtca actttgattg gagcttttca cttttataac acatcaatga
     3061 ttaagctgat gttttactat tattaattaa attgccttct gcgtcgtata gttctggttc
     3121 agtttcttca ttaattgttt gtgctgtaat aactaccttc gttacatttt cgttagaagg
     3181 cacatcaaac ataatatcga ttaacgattc ttctatgatt gaacgtaaac cacgcgcacc
     3241 tgtttttctt tcaattgctt tttcactaat tgctgataaa gcttcttcag tgaactctaa
     3301 atccacatca tctaattcca gcattttagt atattgtttc acaagtgcat ttttaggttg
     3361 cgttaagatg tttttcaacg cagttacatc taatgtttct aaattagcta caattggcac
     3421 acgtccgata aattcaggaa tcaaaccata ggcttgcaaa tcttctgggc gaatttgtgc
     3481 taataatgct tgttcgtcat atttatcagc ttcattgctt gagaaaccaa taactttttc
     3541 accaagacgg cgcttaatca cttcttcaat accatcaaag gcaccaccaa gaataaataa
     3601 gatatttgtt gtatcaattt gaatcatttc ttggtttgga tgtttgcgtc caccttgtgg
     3661 cggaacactt gcagtcgtac cttctaagat tttaagcaat gcttgttgaa caccttcacc
     3721 tgaaacgtca cgtgttatag atgtgttttc agatttacgt gcaattttat caatttcatc
     3781 tacataaata ataccttttt cggctttatc aatgtcaaag tcagctgctt gaattaatct
     3841 caacaagata ttttcaacat catcgcctac ataaccagct tcagttaaac ttgtcgcatc
     3901 tgcaattgca aatggtacat tcaacgtctt ggctaatgtt tgagctaata atgttttacc
     3961 actacctgtt ggcccaatta atgcaatgtt acttttttgt aattcaacat catcttcttt
     4021 tggtcctaat tgttgaatac gcttatagtg gttataaaca gctacagcta aagatttttt
     4081 agctttttct tgaccaataa catattcgtt taaatgatcc ataatttctt taggagtagg
     4141 taattctgtc atcgcttcag aagtgttttg agctaattct tcttcgacga tttctgagca
     4201 taattcaata cactcattac aaatatatac accacttcct gctacaagtt tttttacttg
     4261 atcttggtct tttccgcaga aagagcattt caaattttct tcatcttcat tgaatttaaa
     4321 cattcttttt acacccctat tcgttaaaga ctatactaga ttggatatta caatgcaaca
     4381 tattaacata caaacttttt gcttaaagaa tagtagcaga tacataagct atgcatctgc
     4441 tactcatttt tgtaatttaa tatttaatga agattaatct tctttagttc cttcaacgaa
     4501 ctttgcgtta tctcttaata aatcgataac tttttggata cgaacatcat ttttaatgat
     4561 atcagtatta cctaaagtat ttttgatatc ttcaactgag atattaaatt gtttactcat
     4621 tttttctaat tctttatcga tatcttcatc agtagcttcg attttttcag cttcagcgat
     4681 cgcagttaaa gttaagttag ttttaacacg ttgttctgca tcgtctttca tttgctctct
     4741 taattgagtt tcatcttgac ctgagatttg gaagtacgtt tgtaaatcta aaccttgttg
     4801 ttgaattctt tgtgcaaatt cagacaccat acgatctaat tcagtattaa ccattgcttc
     4861 aggaatatcg attgttgtat tatcagtagc ttttgtaatc gcttcttctt tttcaacatt
     4921 ttcagcatct gtagcttttt gttcagctaa acgtttacgt aagttttctt tgtactcgtc
     4981 tactgtattt gcttctgcat ctaattcatt agcaatttca tctgttaatt ctggaacttc
     5041 tttaaattta atttcgttaa cttttgtttt gaaagttgct tctttaccag ctaattcttc
     5101 agcatggtat tcttctggga atgttacgac aacatctttt tcttcgtcaa ctttcatacc
     5161 ttctaattgc tcttcgaaac caggtatgaa tgaacctgaa ccgatttcta aatcgtaacc
     5221 ttcagcttgt ccaccttcga attcttctcc gtcaactgaa ccactaaagt caatgttaac
     5281 tgtgtcgcca ttttcaacaa caccatcttc tttaactacc atttcagcta aatgtcctaa
     5341 gctgtggtca atcgcttctt gtaactcatc atcagataat tcagtttctt gtttttcaat
     5401 ttcaagacct ttatagtctc ctaatttaac ttctggctca actgtaactg ttgcttcaaa
     5461 aatgaaatct ttaccttttt caatttgagt aacacttact tctggttgtg caactggttt
     5521 aatatcagtt tcgtcaattg cttcaccata agcatctggt aataaaatgt cgatagcatc
     5581 ttgatataat gcttctacac caaagcgttg ttcaaaaatt ggacgtggca ctttaccttt
     5641 acggaatcca ggtacgttaa tttgtttaac cacttttttg aatgcttgat ctaacgcttt
     5701 gtttactttt tctgcaggaa cagtaacagt taataaacct tcgttacctt cctttttttc
     5761 ccaagttgct gtcatgtata tatacctcca tgattaacta atttattttt tcaacttccc
     5821 tattatatca taggtctatt ccctatacaa acattgaaat cacaacgttt atatatttgt
     5881 aaatcaactt ttttcgtcaa aactatatta aaatgctgag tattattttg caacaattta
     5941 actattatta ttcaaatcta attgctgaat aaaattaaat gcatcgatac tttgtaattc
     6001 acatttaagt cctaacatat ttttgaaata acattcgtat gcattaatcc atttattcgt
     6061 ttcaaacaaa gtttcaatat caattggata aatcatgata gagtgattat tcataatatg
     6121 gtgtgcttct tcagcgatat gtaatgcacc atcatttaat gtttccataa cgttaggtat
     6181 aactttttct ttcagtgttg tatgttctag cccatttaaa ttagctggta caaaagttac
     6241 atccatacca tacttttcaa ttgtcagttc ttgtgtacaa tttgcaaacc ttaaatactc
     6301 aatcattaaa ctaatgaggt tataactgta cgtattagat tttaatatat ataataccgt
     6361 ttcttgaaat tgaaaatgac cattgtctat taacttcaaa atcaagtgcg tctgttccct
     6421 cattgataac gtatcaaaat cagttaatga ctgagtcaat cgtttttcat cttcaattaa
     6481 ttttgactta gcaaattctt taagaggatg taatgccatt cttgttttgt gatctttgac
     6541 ttcatcaata atttgatgaa ttacttctac cgcttcaaaa tattgtccca aaccaatcaa
     6601 acttttcacg taataaatca tcaatgcatc atattgttgt atgccagttt tcaataagac
     6661 aattgtttct tctcttaatt caagaaatga ccccgtctca tataacatac ggcatttcat
     6721 cattgctatt tcttcagtga gttcaaattg acgttcatat tgcacaatat attcatacat
     6781 caggtcatag ttttggtctt gttcagcacg ttttatatct ttatataact ttttcgaaga
     6841 gttaggaaac tgaataatat ctgacatata tgtcacctcg tttgattata aatcttgaag
     6901 gattaatgcg ctccacgttt cgaaatcatc atatgactct atgttgcctt tttcaaccca
     6961 ttttattcgt aaagtatctg tttctttagc ttctacatca ttcgtactta cagtgatttt
     7021 aaatacaaca ccaatatgta ccttgcccac ttcattatta tcgtcattaa taaaaccgat
     7081 atattccata ttttgtgaat cttgctcact taaacctact tcttcttcta attctctctg
     7141 tgcattaact ctcaatactt cgttaataga ttctgctcct ggaacatcat tcatatgacc
     7201 gcctacacct attgaagatt gtccatgcaa tcgagcttct ccaccgccag ataatcgttc
     7261 atacactaat atctcgccat gctcattttc aagtaaacaa taagaaatga gttgtttata
     7321 tgatggatcc tcttccatat cgccgcgtcg cttaacttca tattgactta gcgcatcaaa
     7381 aatattttga ccttctggtt tattcttatt taaaaaccca ttgaaagtat tcttttcatt
     7441 attaaaaata atttctctag gtactacaat gatttgttca tcaaatttag acatcgtata
     7501 aaactcctca ctttaatgtt tgttctctta aatgcatttt agcaaatatg tcatatttta
     7561 gcgattaaat gtggcatttt gcttttaaaa aaataaaaaa gagatgacaa tgccatctct
     7621 tttttataag tgttattatt ttaaagcatc tttagcttta gttactaatt gagcaaatgc
     7681 tttttcgtca gaaattgcga tttctgataa cattttacgg ttaatgtcga taccagcttt
     7741 tttcaaaccg ttcattaaac gtgagtagct catttcatgt tgacgagctg ctgcgttgat
     7801 acgtgtaatc cataatttac ggaagtcacg tttacgttga cgacggtcac ggaaagcata
     7861 ttgacctgat ttcattactt gttgcttagc tactttgtat aatgtatgtt ttgaaccgaa
     7921 gtaaccttta gctaatttaa tcgttttttt acgacgcgct cttgttactg ttccaccttt
     7981 aactcgtggc ataaaaaatt cctccttagg taattcctac cgatttctgt atttgttctt
     8041 attttttgta tgctaataat tgttttacac gtttcatatc gctcttagac actaatctag
     8101 ctttacgtaa ttgacgtttt tgtttagtgc tcttgtttgc gaataagtga gatgtgaaag
     8161 ctcttgaacg ttttaattga cctgaagcag ttcttttaac acgtttagct gctccgcggt
     8221 gagttttcat ttttggcata atataagtcc tcctgtaata gctttaatta tttttcagct
     8281 gttggcgcta acatgataaa catttgacgc ccgtccattt taggtttttg ttcaactgtt
     8341 gctatatctt tgcattcatc tgcatatttt tctagcacac gttgaccaat ttccttatgc
     8401 gtaatggcac gccctctgaa acgaatagat actttacatt tatcgccttt agttaagaat
     8461 ttacgtccgt ttttcaactt agtttggaaa tcatgttcct caattgttgg acttaaacga
     8521 atttctttaa cattgataat tttttgtttc tttttcattt ctttttcttt tttctgttgt
     8581 tcgaatttga atttaccgta atccataatt cttgcaactg gtggtttcgc attcggtgca
     8641 acgaccacta agtctaaatc tacacgttca gccatttcta aagcttcacg ctttgattta
     8701 acaccaattt gttcaccatc ttgaccgatt aaacgtaatt cttttgcacg aattttgtca
     8761 ttgatttgag tttgatcttt tgctatggtt gacacctcca aaatttttac gaaatttgca
     8821 ccaagcaaaa aggaagagca ggtataaaat acccgctctt ccttatacac agttatgtgt
     8881 aatgtgatta acctgccaac tgctttatgc gtcgctacag gtgagaagcg ggtgcttcta
     8941 cttggttcgt ttcgtattca acgttgttaa tcatatcaac aattcacatt taagtcaaca
     9001 ctataactgt aattattttt attttaacct tttatttcat ccattgacac gtcttgacgt
     9061 aaatctactt gttctaatgg aatttttttc gttttatatc gaagcttatg ataaataaag
     9121 aatgctaaaa atactggaat tcccatatac gtaattaaga agcgactaaa attaaaatct
     9181 cctgttttaa taaagtcaac atcttgacca ataattacta caatacataa aaagccagca
     9241 aataatggtc cgaatgggaa taatttagca gtatatttta atttagattt gtcataattt
     9301 tgtttatcaa atgctcttct aaatcgataa tgacttactg ctatacctac ccaagcaata
     9361 aaaccagtta aaccacttgc tgcaacgata tattcgtatg caccttttgt aaggctttgt
     9421 aatacgaaaa taatcactac aatgattgct gtaacgagta acgacatata tggcacaccg
     9481 tttttatttg ttttaccaaa tgcttcaaac gctaatttat ctttactcat tgaatatagc
     9541 attcgggttg aagcatacat acctgagtta cctgctgata acacagacgt taaaatgact
     9601 gcattcataa atgatgctgc aaacgcaaat ccagcatttt taaacactaa tgtgaatgga
     9661 gacgttgcta cattatcact accacccatt aatgcactac tatcataagg aattaacata
     9721 ccgataacaa aaatggctaa aatgtaaaat aataaaattc tccagaatac ttgtttaatt
     9781 gctttcggca cagcacgttc aggattttct gattcaccag ccgtaatacc aattaactca
     9841 gtaccttgga atgagaaacc agcgattaag aatacaccta gaattgataa caaacttcct
     9901 cctaagttgc caccaagaat aggaccttca cctttattaa atatttcaaa tcctacaaca
     9961 tgaccaccca tgattcctac aatcgttaat aaaccaattg caatgaaaac aataactgta
    10021 atcactttta tcaatgccaa ccagtattca ctttcaccat agacgcgaac tgataacgaa
    10081 ttcagactaa aaattataac taaaaacaac gcactccatg cccaagcggg tatgccttgc
    10141 aatggtgtcc aatattgaat gacttgtgct gcaatcgtaa tatctgctgc tacagttact
    10201 acccagttaa accaatagtt ccaaccaagc gcaaacccta aagatggatc aacaaatctt
    10261 gtagcatatg tactaaatga acctgatact ggtaaatacg tagccatttc accaagtgac
    10321 gtcattaaga aaaataccat tattccgata attgcgtatc ctattaatgc acccaaagca
    10381 cctgcatcat gaattgctcc accagaagtt acaaataaac ctgtaccaat acaaccccca
    10441 atcgcaatca tagaaatatg acgatcttta agtccccttt tgacaacatt gttactttca
    10501 ttttgaactt ttgacatata agctcccttt ccatgtttta gaggccaaga aataaataac
    10561 ctttattcac tttcataccc ttttttacag aacatagtga tattaaaagt aaaaaagtgg
    10621 ctacatacct ttaagatatg caaccacttt ggcaattatc tatataaggt agcacatcac
    10681 attctgtgac agtacagttc ctattcgaaa actgtcccaa tagatataat ttatggttta
    10741 tatctatttc ggcatcttta cctttcactt gttcaactta tgtaccataa atacttctga
    10801 caagttacta attaaacatg caacctctaa ctcaatttaa tattttaact aacttgtaat
    10861 atacaggatt catcacgcat aatcaaccct gtaaaacttg atacgcaata aaagttttaa
    10921 agcattttat tgcgacaact gtctatctat gttttttcaa acgaatttca tcaactagat
    10981 tccagataaa ttcatctttt tcaactgttt cttggtcttg cgatccatat tgacgcacat
    11041 tcacttgatt attttcaact tccttatccc caactacgat ttgataaggt attttttgca
    11101 tttgagcttc tctgatttta taacccattt tttcattacg gtcatcaata cttacacgaa
    11161 caccttgaga tttcaattca tcttgcaatt ggcgcgcata atcataatgt aaatcaacgt
    11221 taactggaat gatttgaact tgttttggcg ctaaccaagt tgggaatgca cctttagttt
    11281 cttcagttaa aaatgctaca aatcgttcca ttgttgatac aacaccacga tgaataacaa
    11341 ctggacgatg atgttcacca tcttgaccaa tataagttaa atcaaaacgt tctggtaata
    11401 agaaatcaag ttgtgctgtt gataatgtct cttctttacc catcgctgtt ttaacttgaa
    11461 catctagttt cggaccatag aatgccgctt caccaatcgc ttcttcgtac gataagccaa
    11521 gctcatccgc tgcctcttta agcatatttt cagctttatt ccacatgtca tcatcatcaa
    11581 agtacttttc tttatcttca gggtctctat aacttaatct aaagctataa tcctcgaaac
    11641 caaagtcttt atacacatca ataatcatgt ttacaacgcg tttgaattct tctttaattt
    11701 gatcaggtcg aacaaagata tgtgaatcat ttaaagtcat accacgaaca cgttgtaatc
    11761 ctgatacagc accacttgct tcatatctat gcatcgttcc tagctcagcg atacggatag
    11821 gtaattcacg atatgaatgt ggtttattcg cataaatcat catatgatgt ggacagttca
    11881 ttggacgtaa taccatagat tcagtttcat ctaactgcat tggtgggaac atatcttctt
    11941 gatagtgatc ccagtgacca gatgttttgt ataaatcaac attagcaagt actggtgtat
    12001 aaacgtggtc ataacccatg ctcacctctt tatcaacaat gtaacgttca atttcacgtc
    12061 taattgttgc accgttaggt aaccataatg gcaaaccagc accaactaat tggctatttg
    12121 tgaataattc taactcttta ccaattttac gatgatcacg ttctttacgc tcttctaaca
    12181 tttgtaaatg tgcttttaat tcttttttat caaagaaagc agtaccgtat atacgttgta
    12241 acattttgtt gttactatct ccacgccagt atgcacctgc tgtagataat agtttaaact
    12301 ctttaatttt agctgttgat ggaacgtgaa ctccacgaca taaatcagta aaatcacctt
    12361 gactatataa tgttacattt tcatcttcag gaatcgcgtc gattaattct aatttgtatt
    12421 catcattgct gaataactct ttcgcttcat ctcgtgaaac cacttttcgt tcgattttca
    12481 tattttcgtt aacgatttgt ttcattgttt tttcaatttg ttcaaagtca tcagatgaga
    12541 tgttttggtc aatgtcgaag tcatagtaga atccaccttc tattacagga cctacaccaa
    12601 atttaacatt accatataac cttttaatcg cgtgtgccat taaatgtgca gtagaatgac
    12661 gtaatacctc taacgcttct tcactacctg gtgtcacaat tccaattgat ccatcagttt
    12721 caagcggttt agttaaatct acaagttgcc cgttaaattt gccggcaaca gcttttttac
    12781 gtaatccagg actaattgat tgtgctatat cttcagtagt agtaccttta tcaaacgcct
    12841 ttttattacc atctggaaat tgaatattaa tttgttccat gttaaaccct cctatttgct
    12901 ttataaatac ttttatctga cctcgttaaa aaattgaaca acaatcatcg ttttaacatt
    12961 aaacctatta acaataaaca gggaatatat gacaccaaca aacatttatg tctgaaaaaa
    13021 acaaaaaatc ccgcccctat acaagggacg agatcgtcgt ggtaccaccc tagttattta
    13081 atacaattta tgtattaaat atctctgctt aagataacgg tcttgatccg ggtattcatt
    13141 acaaatacca taaatgaagt agtaatcatc taatttatta accatattct catcaaacaa
    13201 tggctttctg tgtaatattg attagatcat cttgtcttca atcatttaaa cgatttagat
    13261 ttgtattcat tatacaccaa tcattttaaa attcaattgt ttctgaaatt ttctcctgat
    13321 aaaaagtatg gtgttgacaa agatttgaca cgttcaataa tacgtgctgc tttagtcttc
    13381 tcttcaccat cacgagtcat cgctaaatga tgttccaatt cactatagtc aaaattagaa
    13441 ctaaagaatg ttggtaattc atgaaccatt cgatagtgta gcaaaggtcc aattacctca
    13501 tctctcaccc atggagtcac ttcttcagcc ccaatatcat caagcattaa aatgtttgct
    13561 tctcttacgc gatgtaattt cttttcaaaa gaaccatctt taaagccacc ttttaatgtt
    13621 ctaataaatt ccggtaaata aataattgtc gaacgtacct tcttagattt gagctgattc
    13681 gcaattgcac ctagaataaa agatttacct gtcccaaatg gaccataaag gtaaaggcct
    13741 ttcacttgtt ccccattagt tattgctgta caaatatcat ctgctgccat agctacatca
    13801 agacggtctc gatgattcat ataaatatct ttcaatttgg catttaaagt atctcgttgc
    13861 atatgatgag atgtaattag ctcagcttca aagcgttctt cgtcgtactt gattttacat
    13921 gggcattgta aatagcgtat tttaattcgg ttattatcaa catataactc aggcacatgc
    13981 ccctttacga aatttggaca atcagcaaat ttatgaccgt cataatgttt ttgttgatct
    14041 ttatactctt gtaacacatt taagtcttca tcaatcatag cattcgttaa ttcagctcga
    14101 tgcgcttcca aaaattgctt aacatctggg tcattgatta cttctttttt tatcttttct
    14161 attctttttt caaagtcctg cgacgtgtta attatacttt taaattgctt cattatcgac
    14221 tgtcctcctc ccatttttta gataatttat ctagaaatgc ttgtcgatct tgctctaatt
    14281 gttgatcatc tacgctatta tctttagccg aatcttcttc actaggttta tctctatttt
    14341 ctaaccattt aggtgttttt tcttttgaaa tacgattacg ttgcccatag tatgaaccac
    14401 gcttttggta atttccgcta gaaccctcat ttttaggttg attaactttt ttagcgtaat
    14461 tatatgcttc tttagctgtc ttaatacctt ttttcttcca atttgatgct atttccaaaa
    14521 tatacgcttt aggaagtttc atatcttctt ttaacatgac aaattgcaac aaaatattaa
    14581 tgacgccaaa agacattttt tcacgttcaa ttaattcttc aaccattgtc ttttgcgata
    14641 tagttggttc tgattcagac caagaagcta acatatcaat tggactcgtt tgttcaagta
    14701 actcaaacca ttcatcactt tgtggctttg gattcacttc tgaagatttg cccgtcgaag
    14761 atgatgtagc aggagatttc acctgtaatt taggcatttg attttcgtgt tccattaaat
    14821 aatacgagcg tgcttgttta cgcatttctt caaaggataa ctgttgtcca cttgtaattg
    14881 aatttaaaat aacatgcttc atgccatctg ctgttaaacc atataaagtc gcgagttgtg
    14941 taattaaacg ctttgcatct ttggtaacaa tgtcttgact aataaaatgt ttacctaaca
    15001 tttgtctcaa catttcaaag tcaaaagatt catttgataa atcgatacct tggtacggtt
    15061 cattaatcgg aatatcactt gtatcgatat ctatttttgt agacggcact ttaaaaacat
    15121 cagtaaattg tcttgttacc tgtttaaatt cactcaaatc aatttgttga tactcaaagt
    15181 atttcttcaa ctcatgaaat cgacgatgct cgacttcact atataaaaag attgacaaca
    15241 ttggatcatt aaaaaataaa tgtgctgaag gcggttgaat taattggtaa acaaattgtg
    15301 tttcttgttc atcatgtttg acaaacgcct ttaacaatcc aatcgcttca agtaagtcca
    15361 tttgttgtct aaactctagt aaattaattt taagttcatt cataaaaata taatgagaaa
    15421 gaatcaatgt ttcattatga ctgtctttaa cgaattgagt cataaaatga tataaaccca
    15481 ctgcttgcgt tccaattagc ggtgtataca gtcgattcaa tacctctaaa tgattcgtat
    15541 ttaaatcaaa gtgttgcata actttgaatt gatcctttgg tcttaagccg aattcgaagg
    15601 cttgtcgtcc catttaagca tcactccgtt tgttttcgct taaaatccct tgcatcgatg
    15661 ctaacaattg atcaacatct ttaaattctt tatagactga tgcaaatcta acatatgaaa
    15721 cttgatcaac atgcattaac aagttcataa cgtgttcacc tatatctcgt gaagacactt
    15781 ccgtatgacc ttcatctcgt aattgccatt caaccttgtt tgttatgtct tcaagttgtt
    15841 gatatctaac tggtcgtttc tcacaagaac gcacaagtcc attaagtatt ttttctcttg
    15901 aaaactgctc tcttgtgcca tcttttttca caactataag ctgactaact tcgatatgtt
    15961 caaatgtagt gaaacgtgtt ccacaatttt cacattctct tcgtcttcga atggcattta
    16021 attcatcggc atgccttgaa tctacaactt tagattgtgt agaattacat ttcgggcatt
    16081 tcattacatc accctcttta ttttgattat gcctaattat actataaatc tagagatgaa
    16141 aaaagaatcc ctcaatttaa ttcatttaac caaataatga aacaataaaa aacattatat
    16201 cgttacttat taagtaattt gcatgacaat attattgtat taaaaaataa aaaacctaac
    16261 tccgaagtca gagttaggct ataaattaat tgtattaact tgcacttaca gtttcttttg
    16321 atgtcaaaag tgctccaatt tgctcagcaa catctacaac tctatttgaa taaccccatt
    16381 cattatcata ccaagcaata acttttactt tattccctga catgaccatt gttgattttg
    16441 catcaataat agctgaattt ggattagtat taaaatcaac agacactagt ggttgatgtt
    16501 cgacttctat gataccttct aaacctgcat tttcaaaagc ttggtttact tcttctgcag
    16561 ttacttcttt ttctaaatca acaactaaat caacgagcga tacattcttt gttggtacac
    16621 gtaatgccat gccgtgtaat ttaccttcta attctggtaa tacttctttt aaagctttcg
    16681 ccgcaccagt agaagtagga ataatgcttt cattacatga acgtgcacgt cttaaatctt
    16741 tatgtggatt atcaatattt ttttggtcat ttgtaatagc gtgaacagta gtcattaaac
    16801 cattaactat tccaaactga ttatttaaaa cttttgcaac tggaccaatg caattagtag
    16861 tacatgaagc attactaaaa atgtcaaatg cttctatatc taattggtta tcatttacgc
    16921 ctttaactac catttgaaca tgtccacctt ttgaaggacc agttaacaat acttttttgg
    16981 cacctgcttt aatatgtgcg atggctttat caccatgatt aaatttacca gttgcatcta
    17041 tagcaatatc gatatctaat tctttccatg gcaagttttc aggattgcga tcagcaacca
    17101 atttaatttt atgatcacca acttgcaatc cattttcaat cggttcaact tttagattat
    17161 attttccatg tgttgtatcg taattgatta aatgtgcaat tgtttcgggt ggataactag
    17221 catttatcgc tactacattt aaatttttat tttgtaatgc aatacgtaat accattcttc
    17281 caattctacc cataccatta attgcaatat tcgttgacat taaaagcacc ccttgatttt
    17341 tatatgttat actttatgga attagtatag catattatat gaattttgaa agcgttttcc
    17401 gttgatatat ttatcttttt tataaaaaat agtcattact tacatacatc aacgcgtacg
    17461 taaagtaatg acttatttat agtgtaatat taatcttctt ctccgtaatt cggcttttca
    17521 atataacctt cttcttctaa caatctctca aggttttgtt ttaattcaag tttatcccct
    17581 aaattatcga taacatgatc ggccattcgg ctttttttat caatagaaat ttggctatag
    17641 acacgtgctt tcgcatcttc taatgacaga ttattacgtt gcattaaacg atccatttgt
    17701 atactttcag aagtgtatac aacccacact tcgtctactg tattttccaa ttcattttca
    17761 aataataatg gaatatccat gattacatta tatccttgtt ttaaatattc ttgcttttct
    17821 tcttccataa tatctcgcac gataggatgt atgatagcat ttaattctaa gcgtttttct
    17881 ggatggttaa acactaaatc acccatataa cgacgattca tctcaccatt ttcatcaatt
    17941 gcttcatcac caaagacttc tcgtacttga gctaaacctt tactcccctt tttaacagct
    18001 tccctggctg ctttatcagc atctactact ttaaaaccga atacggataa gagttctgat
    18061 actgttgatt ttcctgaggc gattccacct gttagaccaa taactttcgg cataatttca
    18121 ctctttcttt atttttgaca tactggacaa taatgactat ttcttgtcgc gatgattttt
    18181 gtttcaattt gacttccaca cactttgcat accggctgct tatatacatt aagatgcaat
    18241 tgcatctcac cagtttttcc atcagcatga cgataatctg aaatacttgt accgccatgc
    18301 ttaatacctt cttctagtac ttctctaaca taataaaaaa ccatttcttg ttgttggtgt
    18361 gttaagtctt ttactttttt atctggtaaa acacctgcac gaaacaacgc ttcacatgcg
    18421 taaatatttc cgcaacctgc gattacttta tgatccaaaa tcacttgttt gattggttta
    18481 ttcttattag actgttgatg aattcgattt aaataatacg tcaatgcttc atttgtaaaa
    18541 ggttcaggcg ctatttctaa aaatgaagga taagatgcta cagacgcaac atttctaatt
    18601 tctccaaaac gacgtatatc tgaataaatt aactttttgt catttgacaa ctcaaaaata
    18661 acatgccaat gcttacgata attaggtatc ataatatctt caagttcatc tacaatgaaa
    18721 aaaccacccg ccatacctaa atgactaatt aatgtacgtt gttctcgttt attatctagc
    18781 tgaaaaacga tatatttact tcttcgttct acatttgtaa tggtatagcc ttccgataaa
    18841 gttttaaaag tatctaattc aattcctttt ataattgttt ccttgccttg agctttacct
    18901 tcgattactt tatccgaaaa tataacgtgt tcaatttttt gatttataac gtagggttca
    18961 attcctcttt ttacatgttc tacttctggt aattcgggca taccattaac ctcactttat
    19021 tttgcatcat accaagttgc accataactt gagtctactt ttaatggaac atctaattgc
    19081 aatgcatttt ccattatctc ttctacaaat tcactaaatg aatctacttc tgacttaggt
    19141 acttcaaaaa ttaattcatc gtgtacttgt aataatagtt tagcttgata tgttgtctct
    19201 ttcatttttt gtgcaaattt aaccattgcc agtttaatga tatctgcagc actgccttgt
    19261 attggcgtat tcatagcagt acgttcagca aagccgcgta aattaaagtt acgactcgta
    19321 atatcaggaa tatagcgtcg acgatgtagc aatgtttcca cgtaacctaa agctttggca
    19381 tcttttacaa tatcagacat atattgtttc acacctggga aactagctaa ataatcatca
    19441 atgaatgctt ttgctttttt acgagtaata cctaaacttt gacttaaacc ataatcactt
    19501 atcccataaa caattccaaa gttaaccgct tttgcttgac gacgcattaa actatcgact
    19561 tgatcagcct ctacaccaaa tactttcata gcagttgctg tatgaatatc atcgccgttg
    19621 ataaatgctt ctttcatact ctcatcttgt gtaatgtgtg ctaatacacg caattcaatt
    19681 tgagaataat ctgctgataa tataacgcta tcttttgaag ttggtttaaa ggcttttctt
    19741 attttacgcc cttcttcaag tctaaccgga atattttgta aattaggatc tacacttgat
    19801 aaacgtccag tttgcgctaa agtttggtta aaacgtgtat ggatacgttg atcatcacta
    19861 attacctttt gcaatccttc aacataagta gactgtaact tagataattg acgatattct
    19921 aaaatataat caatgatagg atgttcacct tgcaattgct ctaagacatc tacagcagta
    19981 gaatatcctg ttttcgtctt tttaataaca ggtaattgta atgtctcaaa caatacaaca
    20041 cctaattgtt taggagaatt tatattaaaa tcttcaccag ctgcatcatg gatatttcga
    20101 atcaagacgt ctaatttttc ttgaatttct ttttccattt cttctaaatc atgaacatct
    20161 gtaaatatac caatttcttc catttcactt aaaatcttag ctagcggtag ctctaaatca
    20221 gctaagagtt ctacctgatt gtattcttct aattgtttat ccatatttgg tttcgcaaag
    20281 taaattgcat cagtaataga agcaacatat ggatttaaaa catcatcttc aggtacctta
    20341 aatttcttac cttttccata tatacttaca tcgtctttca caaaactttg accgtacaat
    20401 gaaacaactg attgaacatc actaatcgta cgagatggat caattatata actggccaac
    20461 ataatatcga aagaaatatt ttgaatatca attcccaatc tatgtgatgc tacatatgtt
    20521 tttttagcat catatacgac ttttttcgta ttcggatttt ctaaccatga aactagttcg
    20581 gcataattat ttatgtcatc cgcattaatt acaatatgtt tctcacctgt aaataaagag
    20641 aattttaaaa tattatttcg caaatagtta ccaccgtcta attcgaaatg gatggccgct
    20701 tctttcaatg aagtaaaatc aatattatca aaagacgttt caatttcaaa tgtcttttct
    20761 attgcatctt caacgcttgc tgattgatca atgtcagaca acaattgttt gaattctaac
    20821 ttcttaaaca attcgatttt ttcttgttgt tcatcttgat gagtcattaa cgtatcttca
    20881 agttttactt caatcggact atctacatta atcgttgcta attctttact cattaatgca
    20941 tcttctttgc tattttgaag tttttctttt aactttttac ctgaaatttc atctaaatgt
    21001 tcatagacac cttctactgt gtcaaattgg tttagcaatt ttattgctgt tttctctcca
    21061 acacctgcaa cacctggtat attatcagaa gtatctccca ttaatccttt catatcaata
    21121 atttgattag gtgttaaccc gttgtatttt tccgcaataa agtcaggtgt ataatgatca
    21181 acatcagtaa cacctttttt agtgtaataa atggttacat tatccgttgc aagttgtgtt
    21241 aaatctcggt ctcccgttat aataattgtc tgaaagcccg ctttatctgc ttctttactt
    21301 aaagttccga taatatcatc tgcctcatag ttatctaatt cataacgttt aatatgataa
    21361 gcatctaata attggcgtat ataaggaaat tgctcactta gttcaggcgg cgttttctgg
    21421 cgtccacctt tatattcact atatttttca tgtctgaaag ttgttttacc tgcatcaaac
    21481 gctactaaaa aatgatttgg cttttcttct tttaaaatct tctctagtaa cattgcaaaa
    21541 ccatatactg cattggtatg aatgcctgct ttgtttgata acaaaggtaa tgcataaaaa
    21601 gctctaaaac ttaagctatt accatcgatt aatactaatt tattcacaat tttaacctcc
    21661 agaactaatt tatatatatt ttagcatatt cgactgtgat gtcgtaatta aagaaactat
    21721 ctcatagagt atttatacac gacctaattt caaataaaac aagtgcctat atagacttag
    21781 ctaaaaaata aaaaagacat aatcatgatt gttcttgatg agcactccat aaagtcaggt
    21841 taaaagtcca acttttgaga tgcacattat catttcatga ttatgtctac tttttaatat
    21901 ttttatgctg ctattttaaa tttttatagg taagaataag ttatgaatcg tctgaaccaa
    21961 atggaatata tttaactttg gtcattttcg gtaattcttc ataattatta aaccactctt
    22021 ggtaattttt attgattggt gacggatgat gcatataatg ttcaaacggc aatgattcca
    22081 ctgtataaac ctcttgtcgt ggatgatctt taatttgttg ttttaataac tgaactcttt
    22141 tttcatgttc ataatgaaca taaataaatg cactaagata tatcacagct aaaactagtg
    22201 atgcaccttt aataaaatta acattgattg acttatattt ccgaaattct tttaataaaa
    22261 taattaaaat gattacatgt atcgtataaa caatcaaaaa attacctggt tctattggag
    22321 taacaatgac tagtgtcgac gccgaaacac atattgcaat aagtaaggaa tataaagtga
    22381 tttgtgtttt acgatcatta atagacaaat aaatacctac aaatatcgaa aacgcaaagt
    22441 aaccacatac aattacgttc acaaaaccaa ccaatccaat atctgtattt ttatttaata
    22501 aaaactgatt gtaaaatagt aaataataaa gcggtaaagt gataaatcct atcataatga
    22561 tacgacgctt taatattgtt aaatgtacat accgatcact tctaagcagt agataaataa
    22621 taagcgctgc aatcatactt aaaattataa tctgactgaa aatgacacca tatggaagtg
    22681 acgttgatat catttctgca aattttgaaa agataccttg attatttgaa acttgttgat
    22741 attctgatcc ttcaaataaa attttgcgat aatttggatt ggaaaacata atgattgtac
    22801 ctatagtagc aatcataaat gaaaacaata atttataatt aagtgttcga tttgcagtaa
    22861 attcatacag aaaagctaaa attaaaatga tacagtggaa taatgtcaca ttctccataa
    22921 ataattgacc gaagaagcat aaaacataga atagtacagt aacacttacc ggctgctttt
    22981 ctttatatat aattgcatta atacaataat aaataataaa gagtgaaatt agtgttgatg
    23041 tcgcataatt ataaaatcct gcaaaccagc catatgtatc tgcataaata gcacttggta
    23101 aaattaacat taaagaaaat gctaacaaat aatagcttgt ccacgcttta caacgtgtaa
    23161 tgtgcataat catccaaatg atgcccatac taatgagacc atatgaaagc caacgtaacc
    23221 agcttacatg tacagctata atttcaaaga tatttccgat atagcgacca ttaagtgatg
    23281 caaatcctac ttttaaaata tcagtattat aattgctaaa ccattgtaaa tcatcgtgca
    23341 tgattggtag taagataccc ataaaagtat aaaacaataa tatcgcaatt aatatcaaag
    23401 ttgtcttgtg taattgaatt gttttcactt tgctaatcct caaatctagt taaattttcc
    23461 tcaacttgta ggtcgaaaaa ttaattcaat atttttaatg tatttctaat tttcacctat
    23521 gcatgtttcc tcaatcaaat tagataaaca aggtatttaa tattactttc aacaatttat
    23581 ctaaatcgcc cctcgtcttt ttctatgacg aatgattaca cttgtgtatt caagtgttca
    23641 aa
//
LOCUS       DANBWH010000015        23317 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.015, whole genome shotgun sequence.
ACCESSION   DANBWH010000015 DANBWH010000000
VERSION     DANBWH010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 23317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23317
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.015"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..355
                     /locus_tag="PPY92_000420"
     CDS             <1..355
                     /locus_tag="PPY92_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDJ2624444.1"
                     /translation="PVGYLISFNTQSLSQSVAENIIRVYTVMTIILFVVIHAIILMGI
                     VQYYLKRGQKITKDKVKTADKDKKVVTEPKNTKVENGKVTTSVETKTEKAQDSLNDNT
                     TKTMTSDKPEDNQPK"
     gene            complement(788..892)
                     /locus_tag="PPY92_000421"
     CDS             complement(788..892)
                     /locus_tag="PPY92_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624445.1"
                     /translation="MLLLERTSMSDFEMLMVVLTIIGLVLISTQDHKK"
     gene            1572..1730
                     /locus_tag="PPY92_000422"
     CDS             1572..1730
                     /locus_tag="PPY92_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001792784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDJ2624446.1"
                     /translation="MSKSSDALFNSLTQSFELIEGVYNELVTDNLKIVMYKPRKESTS
                     GRINRRFK"
     gene            complement(2388..3245)
                     /locus_tag="PPY92_000423"
     CDS             complement(2388..3245)
                     /locus_tag="PPY92_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDJ2624447.1"
                     /translation="MDNVKAIFLDMDGTILHENNQASTYTKDVINQLREKGYKVFLAT
                     GRSHSEIHQLVPQDFAVNGIISSNGTIGEVDGEIIFKHGLSLAQVQQITNLAKRQQIY
                     YEVFPFEGNRVSLKEDETWMRDMIRSQDPINGVSHSEWSSRQDALAGKIDWVTKFPEG
                     EYSKIYLFSSNLEKITAFRDELKQNHVQLQISVSNSSRFNAETMAYQTDKGTGIKEMI
                     AHFGIHQEETLVIGDSDNDRAMFEFGHYTVAMKNARPEIQALTSDVTAYTNEEDGAAK
                     YLAKHFLAD"
     gene            complement(3313..4095)
                     /locus_tag="PPY92_000424"
     CDS             complement(3313..4095)
                     /locus_tag="PPY92_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDJ2624448.1"
                     /translation="MLIQLDQIGRMKQGKTILKNISWQIAKGDKWILYGLNGAGKTTL
                     LNILNAYEPATTGGVNLFGKMPGKVGYSAETVRQHIGFVSHSLLEKFQEGERVIDVVI
                     SGAFKSIGVYQDIDDEVRNEAHHLLKLVGISAKAQQYIGYLSTGEKQRVMIARALMGQ
                     PQVLILDEPAAGLDFIARESLLNILDSLSDSYPTLAMIYVTHFIEEITGNFTKILLLK
                     DGESVQQGLIDDILTSENMSRFFQKNVAVQRWNNRFSMAMLE"
     gene            complement(4327..6132)
                     /gene="glmS"
                     /locus_tag="PPY92_000425"
     CDS             complement(4327..6132)
                     /gene="glmS"
                     /locus_tag="PPY92_000425"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDJ2624449.1"
                     /translation="MCGIVGYIGYDNAKELLLKGLEKLEYRGYDSAGIAVVNDDNTTV
                     FKEKGRIAELRKVADSSDFDGPVGIGHTRWATHGVPNHENSHPHQSSNGRFTLVHNGV
                     IENYEELKGEYLQGVSFISETDTEVIVQLVEYFSNQGLSTEEAFTKVVSLLHGSYALG
                     LLDAEDKDTIYVAKNKSPLLLGVGEGFNVIASDALAMLQVTSEYKEIHDHEIVIVKKD
                     EVIIKDADGNVVERDSYIAEIDASDAEKGVYAHYMLKEIHEQPAVMRRIIQEYQDAEG
                     NLKIDQDIINDVKEADRIYVIAAGTSYHAGLVGKEFLEKWAGVPTEVHVASEFVYNMP
                     LLSEKPLFVYISQSGETADSRAVLVETNKLGHKSLTITNVAGSTLSREADHTLLLHAG
                     PEIAVASTKAYTAQIAVLSILSQIVAKEHGREADIDLLRELAKVTTAIEAIVDDAPIM
                     EQIATDFLETTRNAFFIGRTIDYNVSLEGALKLKEISYIQAEGFAGGELKHGTIALIE
                     DGTPVVALATQENVNLSIRGNVKEVVARGAHPCIISMEGLEKEGDTYVIPHVHELLTP
                     LVSVVALQLISYYAALHRDLDVDKPRNLAKSVTVE"
     gene            6813..8351
                     /locus_tag="PPY92_000426"
     CDS             6813..8351
                     /locus_tag="PPY92_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDJ2624450.1"
                     /translation="MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFID
                     NGWLPNKDLATLAGPMITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPM
                     LLGAMIMGPLVGWLMKKTDQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLM
                     KFIMHILSVAVEALVHAHLLPLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQS
                     ILYTIESNPGPGLGILLAYMIFGKGTAKATSYGAGIIHFLGGIHEIYFPYVLMRPLLF
                     IAVILGGMTGVATYQATGFGFKSPASPGSFIVYCLNAPRGEFLHMLLGVFLAALVSFV
                     VAALIMKFTREPKQDLEAATAQMENTKGKKSSVASKLVSSDKNVNTEENASGNVSETS
                     SSDDDPEALLDNYNTEDVDAHNYNNINHVIFACDAGMGSSAMGASMLRNKFKKAGIND
                     ITVTNTAINQLPKDAQLVITQKKLTDRAIKQTPNAIHISVDNFLNSPRYEELLNNLKK
                     DDQA"
     gene            8386..10518
                     /locus_tag="PPY92_000427"
     CDS             8386..10518
                     /locus_tag="PPY92_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDJ2624451.1"
                     /translation="MLLSTREKEMIALLIKYHSQYITIHDIAQQLAVSSRTIHRELKG
                     VEAYLTSFSLTLERANKKGLRIAGADSDLNDLKQSIAQHQTIDLSVEEQKVIIIYALI
                     QAKEPVKQYSLAQEIGVSVQTLAKMLDDLELDLNKYQLSLSRKRGEGIYLVGTESKKR
                     EFLSQLMVNNLNSTSVYSVIENHFVFHSLNQIHKDFVDLERIFNVERLLMDYLSALPY
                     QLTESSYLTLTVHIVLSISRIKNGEYVALNDDIYDSVQNTFEHKVASELADKLGQIYD
                     VTFNQAEIAFITIHLRGAKRKNLNDTSLNNRCEENKIKAFVNKVEMISGMTFADLDTL
                     VDGLTLHLNPAINRLQANIETYNPLTDMIKFKYPRLFENVRLALNDCWPDLIFPESEI
                     AFIVLHFGGSIKNQGNRFLNILVVCSSGMGTSRLLSTRLEQVFSEIERITQASVSDLK
                     SLDLSQYDGIISTVNLDIDSPYLTVNPLLPDSDISYVAQFLNTKSTFQETHDKSSNMI
                     DKDDVHVETKDVDGNTSFENEQTSYLTSVFEKHLSDEKSEQLLHHMRSGLTLLDSVKI
                     VSTEVKQWQTYIADYLYQCDVINDPTSFAELLEQRLIDNPGWILSPYPVAIPHLRDNM
                     IKHPMILITVLEEPLTLPSIQNDNQTIKYMISMFIPDNDFMASLVSDLSEFLSLKLES
                     IDTFMENPQELETLLRNKFLERIKKQFI"
     gene            10530..10964
                     /locus_tag="PPY92_000428"
     CDS             10530..10964
                     /locus_tag="PPY92_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDJ2624452.1"
                     /translation="MSELFSNDNIFLNVNVNSQNEAIEKAGKALVDSGAVTDAYIQAM
                     KDREQVVSTFMGNGLAIPHGTDEAKTNVIHSGLTLLQIPEGVDWDGEVVKVVVGIAGK
                     DGEHLDLLSKIAITFSEEENVDRIVQAKSAEEIKQVFEEADA"
     gene            10964..12070
                     /locus_tag="PPY92_000429"
     CDS             10964..12070
                     /locus_tag="PPY92_000429"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDJ2624453.1"
                     /translation="MKAVHFGAGNIGRGFIGYILADNNVKVTFADVNEEIINALAHDH
                     QYDVILADESKTTTRVNNVDAINSMQPSEALKQAILEADIITTAVGVNILPIIAKSFA
                     PFLKEKTNHVNIVACENAIMATDTLKKAVLDITGPLGNNIHFANSAVDRIVPLQKNEN
                     ILDVMVEPFYEWVVEKDAWYGPELNHIKYVDDLTPYIERKLLTVNTGHAYLAYAGKFA
                     GKATVLDAVKDSSIEAGLRRVLAETSQYITNEFDFTEAEQAGYVEKIIDRFNNSYLSD
                     EVTRVGRGTLRKIGPKDRIIKPLTYLYNKDLERTGLLNTAALLLKYDDTADQETVEKN
                     NYIKEHGLKAFLSEYAKVDDGLADEIIEAYNSLS"
     gene            complement(12389..19834)
                     /locus_tag="PPY92_000430"
     CDS             complement(12389..19834)
                     /locus_tag="PPY92_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HDJ2624454.1"
                     /translation="MNLFRQQKFSIRKFNVGIFSALIATVTFISTNPTTASAAEQNQP
                     AQNQPAQPADANTQPNANAGAQANPAAQPANQGGQTNPAGGAAQPAGQGNQADPNNAA
                     QAQPGNQAAPANQAGQGNNQATPNNNATPANQTQPANAPAAAQPAAPVAANAQTQDPN
                     ASNTGEGSINTTLTFDDPAISTDENRQDPTVTVTDKVNGYSLINNGKIGFVNSELRRS
                     DMFDKNNPQNYQAKGNVAALGRVNANDSTDHGNFNGITKTVNVKPDSELIINFTTMQT
                     NSKQGATNLVIKDAKKNTELATVNVAKTGTAHLFKVPTDADRLDLQFIPDNTAVADAS
                     RITTNKDGYKYYSFIDNVGLFSGSHLYVKNRDLAPKATNNKEYTINTEIGNNGNFGAS
                     LKADQFKYEVTLPQGVTYVNDSLTTTFPNGNEDSTVLKNMTVNYDQTANKVTFTSQGV
                     TTARGTHTKEVLFPDKSLKLSYKVNVANIDTPKNIDFNEKLTYRTASDVVINNAQPEV
                     TLTADPFSVAVEMNKDALQQQVNSQVDNSHYTTASIAEYNKLKQQADTILNEDANHVE
                     TANRASQADIDGLVTKLQAALIDNQAAIAELDTKAQEKVTAAQQSKKVTQDEVAALVT
                     KINNDKNNAIAEINKQTTSQGVTTEKDNGIAVLEQDVITPTVKPQAKQDIIQAVTTRK
                     QQIKKSNASLQDEKDVANDKIGKIETKAIKDIDAATTNAQVEAIKTKAINDINQTTPA
                     TTAKAAALEEFDEVVQAQIDQAPLNPDTTNEEVAEAIERINAAKVSGVKAIEATTTAQ
                     DLERVKNEEIFKIENITDSTQTKMDAYKEVRQAATARKAQNATVSNATDEEVAEANAA
                     VDAAQTEGLHDIQVVKSQQEVADTKAKVLDKINAIQTQAKVKPAADTEVENAYNTRKQ
                     EIQNSNASTTEEKEAAYTELDAKKQEARTNLDAANTNSDVTTAKDNGIAAINQVQAAT
                     TKKSDAKAEIAQKASERKTAIEAMNDSTTEEQQAAKDKVDQAVVTANADIDNATANTD
                     VDNAKTTNEATIAAITPDANVKPAAKQAIADKVQAQETAIDANNGSTTEEKEAAKQQV
                     QTEKTAADAAIDAAHSNVEVEAAKNAEIAKIEAIQPATTTKDNAKQAIATKANERKTA
                     IAQTQDITAEEIAAANADVDNAVTQANSNIEAANSQNDVDQAKTTGETSIDQVTPTVN
                     KKATARNEITAILNNKLQEIQATPDATDEEKQAADAEANTENGKANQAISAATTNAQV
                     DEAKANAEAAINAVTPKVVKKQAAKDEIDQLQATQTNVINNDQNATNEEKEAAIQQLA
                     TAVTDAKNNITAATDDNGVDTAKDAGKNSIQSTQPATAVKSNAKNEVDQAVTTQNQAI
                     DNTTGATTEEKNAAKDLVLKAKEKAYQDILNAQTTNDVTQIKDQAVADIQGITADTTI
                     KDVAKDELATKANEQKALIAQTADATTEEKEQANQQVDAQLTQGNQNIENAQSIDDVN
                     TAKDNAIQAIDPIQASTDVKTNARAELLTEMQNKITEILNNNETTNEEKGNDIGPVRA
                     AYEEGLNNINAATTTGDVTTAKDTAVQKVQQLHANPVKKPAGKKELDQAAADKKTQIE
                     QTPNASQQEINDAKQEVDTELNQAKTNVDQSSTNEYVDNAVKEGKAKINAVKTFSEYK
                     KDALAKIEDAYNAKVNEADNSNASTSSEIAEAKQKLAELKQTADQNVNQATSKDDIEV
                     QIHNDLDNINDYTIPTGKKESATTDLYAYADQKKNNISADTNATQDEKQQAIKQVDQN
                     VQTALENINNGVDNGDVDDALTQGKAAIDTIQVDATVKPKANQAIEAKAEDTKESIDH
                     SDQLTAEEKTEALAMIKQITDQAKQGITDATTTAEVEKAKAQGLEAFDNIQIDSTEKQ
                     KAIEELETALDQIEAGVNVDADATTEEKEAFTNALEDILSKATEDISDQTTNAEIATV
                     KNSALEQLKAQRINPVVKKNALEAIREVVNKQIEIIKNADADASAKEIARTDLGRYFD
                     RFADKLDKTQTNTEVAELQNVTIPAIEAIVPQNDPDANDTNNGTDNNDATANSNANAT
                     PENTGQPNVSETTDNGKADASPTTPNNSDAATGETTVTSATDDAKDKPQANNNSSADA
                     STNSPTMDNDVTSKPEVESTNNGTTDKPVTETDNATPAESTTNNNSTTTATNENAPTG
                     STATAPTTASTEAASSADSKDNASVNDSKQNAEVNNSAESQSTNGKVAQPKSENKAKA
                     EKDGRDSTNQSMVESTTETLPSADITEPNVPSNTSKDKEESTTNQTDAGQLKSETNVA
                     SNEADKSPSKADTEVSNKPSTSASSEAKDKMTSTNVSQKDDTATADTNDTQKSVGPVA
                     NNKAKDMQTNDTQKSVGSAANNKATQNDGANASPATVSNGSHSMHQDMLNVTKPEENK
                     ANAKSDQQGKVNKPKQQAKTLPDTGMSHNDDLPYAELALGAGMAFLIRRFTKKDQQTE
                     E"
     gene            complement(20112..21467)
                     /locus_tag="PPY92_000431"
     CDS             complement(20112..21467)
                     /locus_tag="PPY92_000431"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDJ2624455.1"
                     /translation="MGKYFGTDGVRGVANQELTPELAFKLGRYGGYVLAHNKGEKHPR
                     VLVGRDTRVSGEMLESALIAGLISIGAEVMRLGIISTPGVAYLTRDMGAELGVMISAS
                     HNPVADNGIKFFGSDGFKLSDEQENEIEALLDQENPELPRPVGNDIVHYSDYFEGAQK
                     YLSYLKSTVDVNFEGLKIVLDGANGSTSSLAPFLFGDLEADTETIGCSPDGYNINEKC
                     GSTHPEKLAEKVVETESDFGLAFDGDGDRIIAVDENGQIVDGDQIMFIIGQEMHKNQE
                     LNNDMIVSTVMSNLGFYKALEQEGIKSNKTKVGDRYVVEEMRRGNYNLGGEQSGHIVM
                     MDYNTTGDGLLTGIQLASVIKMTGKSLSELAGQMKKYPQSLINVRVTDKYRVEENVDV
                     KEVMTKVEVEMNGEGRILVRPSGTEPLVRVMVEAATDEDAERFAQQIADVVQDKMGLD
                     K"
     gene            complement(21494..22426)
                     /locus_tag="PPY92_000432"
     CDS             complement(21494..22426)
                     /locus_tag="PPY92_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HDJ2624456.1"
                     /translation="MLESKWGLRFIAFLLALFFFLSVNNVFGNIFNTGNLGQKSSKTI
                     QDVPVEILYNTKDLHLTKAPETVNVTISGPQSKIIKIENPEDLRVVIDLSNAKAGKYQ
                     EKYQVKGLADDIHYSVKPKLANITLENKVTKKMTVQPDVSQSDIDPLYKITKQEVSPQ
                     TVKVTGGEEQLNDIAYLKATFKTNKKINGDTKDVAEVTAFDKKLNKLNVSIQPNEVNL
                     QVKVEPFSKKVKVNVKQKGSLADDKELSSIDLEDKEIEIFGSRDDLQNISEVDAEVDL
                     DGISESTEKTVKINLPEHVTKAQPSETKAYINVK"
     gene            complement(22428..23237)
                     /locus_tag="PPY92_000433"
     CDS             complement(22428..23237)
                     /locus_tag="PPY92_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDJ2624457.1"
                     /translation="MDFSNFFQNLSTLKIVTSILDLLIVWYVLYLLITVFKGTKAIQL
                     LKGILVIVIGQQISMILNLTATSKLFDIVIQWGVLALIVIFQPEIRRALEQLGRGSFL
                     KRYTSNTYSKDEEKLIQSVSKAVQYMAKRRIGALIVFEKETGLQDYIETGIAMDSNIS
                     QELLINVFIPNTPLHDGAMIIQGTKIAAAASYLPLSDSPKISKSLGTRHRAAVGISEV
                     SDAFTVIVSEETGDISVTFDGKLRRDISNEIFEELLAEHWFGTRFQKKGVK"
BASE COUNT         6859 a         4128 c         3707 g         8623 t
ORIGIN      
        1 acctgttggt tacttgattt catttaatac gcaatcactc tctcaaagtg ttgcagaaaa
       61 tatcataaga gtttatacag taatgacaat cattttattc gttgttattc atgcaatcat
      121 tttaatgggt atcgttcaat attacttaaa acgtggtcaa aaaattacta aagataaagt
      181 aaaaacagct gacaaggata aaaaagttgt cactgaacca aaaaatacaa aagtagaaaa
      241 tggtaaagtg acaacatctg ttgaaacaaa aacagaaaaa gcacaagatt cattaaatga
      301 taatacaaca aaaacgatga cttcagataa accagaagac aatcaaccaa aataaatttt
      361 ggagcccctt acaactttag ttgttagggg ctcttatgca tgcgcttagc gcaggcgctc
      421 ctacttttca actttagttg ttaggggctt ctttctaaaa taactaagtg aattatctat
      481 atatgaagag gtctccaata ctttttatat aattcgtgat tacattctat acttttaatt
      541 gtattttctg ctgtatggaa agttggtttt attaattctg attatgttca gaactacaat
      601 tcataagaaa aatgtgttaa aatatatttg tagtaagtag aagcaaaaga tgaaaatctt
      661 taactcttga aacacaaaaa gggcaacact cggaaacatg ttaccctaat gagcccgtta
      721 aaaagacggt gaccttatat tttatttaaa aatagccttc aaaatgccgg tcaaagcgaa
      781 tagaaggtta ttttttatgg tcttgagtac taatcaatac taaaccaatg attgttaata
      841 caaccatcag catttcaaaa tcagacatgc tagtcctttc taggagtaac aactataacc
      901 ataggcatca cccctttatt aagagattag ccaccatcta tccaacttgc tcaataaata
      961 ttataacatg cacactttta aacagtaatt catagtataa gttttttcat ttcttaatca
     1021 actcgataaa cagttgagtg acataccctt gtctaactag ctatttcttc ccctgttatc
     1081 gtttgttcat catataaaaa tttaatttct cattttttat tctctgataa agaagagcac
     1141 caaccttttc ggctgatatg ctcccttcaa aatagacatt gaaaaatgaa aactttgaaa
     1201 gagaaatcgt cagtctatta ttgataagta ttatcctatc ctcgaaaaat tactttcaga
     1261 cactagtgaa caaaaatttt actataagcg tatactttgg caatattcga aggaaaaaca
     1321 tggattaacc tgtgcatatt atacatttag agcttatata cttaaacatg atgaatttac
     1381 tccttatatt atgaaatgtt atcaatagat gtcgcctaaa gatacaacaa gattcgagac
     1441 aaaacctgat catcaagcac agtttgactg gaaagaaagc attaacttta aaacgaaaga
     1501 taatcaaagg attccactat atataggtgt gctactactt tattattctc gttttataat
     1561 catgcataac aatgagtaaa tcaagtgatg ccttatttaa ttcattaaca caatcgtttg
     1621 agctaataga aggtgtatat aatgaacttg taacggataa cttgaaaata gtcatgtata
     1681 agccaagaaa agaaagtaca agtggtcgaa taaatcgtag atttaaataa ttcgctgatg
     1741 gttttaaatt tcaagtaaaa ccttgtattt ccggcctccc tagaaaaaga taaagttgaa
     1801 tcaataacaa aaatattaga tgaaattaat gcttaccaag acgaattgca tttggaagag
     1861 attcctaagt ttttttaatc ttaatgaacg atttaactac taagttcata ctagtacagg
     1921 taaattgcct attactgcct aagagaaaga aaaaattttc ttacaaccac tacagaatgt
     1981 gaatgtaaaa aactcatata aagtgaaaca tatcttactt atggaatgaa atgtttgatt
     2041 atcccaaaat cacaaatgcg attttggagt tgttaggggc tctaacgcaa atacacccct
     2101 acttaaaata acggtagttg ttatagtata cttaaatgca taaaccatat aaaaggtgat
     2161 gctaatatga catacggttc aattgtagct atactctgtg ccctatttgt gattttcttc
     2221 attccatata tggagaagaa agacaaaaag aagaagtaac actagtatct ggattactag
     2281 catacaaagg agcagtaaga cattttctaa tagaaaataa ctttactgct ctttgtacat
     2341 tactcatcaa ctattgtaaa ttaaataata aataactacc tattttatta atcagctaaa
     2401 aaatgctttg ctaaatattt tgctgcgcca tcctcttcat tcgtgtatgc cgttacatct
     2461 gaagttaatg cttggatttc agggcgtgca tttttcatag caactgtata atgaccaaat
     2521 tcaaacattg ctctatcatt gtcgctatct ccaataacta acgtttcttc ttgatgaata
     2581 ccaaaatgtg caatcatttc tttaatgcct gtacctttat cagtttgata agccattgtt
     2641 tccgcattaa atcttgatga atttgaaaca ctaatctgta gttgcacatg attttgcttt
     2701 aattcatctc taaatgctgt tattttttct aaattagaac tgaatagata aatttttgaa
     2761 tattcacctt caggaaactt agttacccaa tctatcttac cagcaagcgc atcttgtctt
     2821 gaagaccatt cactatgact tacgccatta ataggatctt gactacgaat catatctcgc
     2881 atccatgttt catcttcttt taaagaaact ctattacctt caaaaggaaa tacctcataa
     2941 taaatttgtt ggcgcttagc taaattagta atttgttgca cttgagccaa tgataaacca
     3001 tgcttgaaga taatttctcc atctacttct ccaattgttc catttgaact aatgatgcca
     3061 ttaaccgcaa aatcttgagg tacaagttga tgtatttcag aatgcgaacg tcctgttgcc
     3121 aaaaatactt tatatccttt ctctctcaat tgattaatga catctttcgt atacgttgat
     3181 gcttgattat tttcatgtaa aattgttcca tccatgtcca aaaatattgc ttttacgtta
     3241 tccatattgc cctccactgt ccattttatc ttaattaaat tttgtcatta ttacttatta
     3301 tttgcaaaat atttactcta acattgccat agaaaatcga ttattccatc tttgaactgc
     3361 tacatttttc tggaaaaatc gtgacatgtt ttcagatgtc aaaatatcat ctattaatcc
     3421 ttgttgtaca ctttccccat cttttaatag caaaatttta gtaaagttac cagtaatttc
     3481 ttcaataaag tgcgtcacat aaatcatcgc aagcgttgga tatgaatctg acaatgagtc
     3541 aagtatattc aatagtgact cacgagcaat aaagtctaaa ccagctgctg gctcatctaa
     3601 aattaaaacc tgaggttgcc ccattaaagc tcgtgcaatc atcactcgtt gtttttcacc
     3661 tgtagataaa taaccaatat attgttgcgc tttagcagat attccaacta atttaagtaa
     3721 atgatgtgct tcattacgta cctcatcatc aatatcttga taaacaccaa ttgatttaaa
     3781 agcaccgctt atcaccacat cgattactct ttcaccctct tgaaactttt ccagtaaact
     3841 atgagataca aaacctatat gttgtcgtac agtctctgca gaatacccta ccttgcctgg
     3901 cattttacca aatagattaa caccgccagt tgtcgcaggc tcatatgcat ttaatatatt
     3961 taatagcgtt gtcttaccag caccattcaa cccatataat atccatttat cacctttagc
     4021 aatttgccaa gaaatatttt ttaaaattgt ttttccttgc ttcattcgcc caatttgatc
     4081 taattgaatt aacatgcgag gtcacccctt tattgctact ccatacttct cataaaatca
     4141 tattaacata acacccttaa ttgtcagact atttaaataa ataaaacact tcctttttac
     4201 gcatttctgc caaattaaga tgaagtaaaa gctaagtcga cctaaaaaag cacccttcta
     4261 gtcgattaat ctaaaagggt gccatatact ttaattttaa tacatgattg attctaaaaa
     4321 agtgaattat tctacagtaa ctgatttagc aaggttacgt ggtttatcca catctaaatc
     4381 tctgtgtaat gctgcataat atgaaatcaa ttgtaatgca actactgaaa ctaatggtgt
     4441 caataattca tgtacatgag gaatgacata agtgtcgcct tctttttcaa gaccctccat
     4501 agaaataata catggatgtg caccacgtgc tactacttct ttaacgttac cacgaattga
     4561 taaattaacg ttttcttgag ttgctaaagc aacaactggt gtgccatctt cgattaatgc
     4621 aatcgtacca tgtttaagtt cgccaccagc aaaaccttct gcttgaatgt aagaaatttc
     4681 tttaagtttt aacgcacctt ctaaacttac gttatagtca atagtacgtc cgataaagaa
     4741 tgcattgcgt gttgtttcta agaaatctgt tgcaatttgt tccataattg gtgcatcgtc
     4801 aacaattgct tctattgctg ttgttacttt tgctaattct ctcaataaat caatatctgc
     4861 ttcacgaccg tgctcttttg caacgatttg agacaagatt gataatactg caatttgtgc
     4921 agtatatgct tttgtagatg caactgcgat ttcaggaccc gcatgtaata acaatgtgtg
     4981 gtctgcttca cgtgataaag ttgaacctgc aacattagtg attgttaatg atttatgacc
     5041 taatttatta gtttcaacta atactgcacg gctatctgct gtttcacctg attgagaaat
     5101 ataaacgaac aatggttttt cagataataa tggcatgttg tagacaaact ctgatgcaac
     5161 gtgtacttca gttggtacgc cagcccattt ttctaaaaat tctttaccta ctaaacctgc
     5221 atggtagctt gtacctgctg caataacgta aatgcggtct gcttctttaa catcattgat
     5281 gatgtcttga tcaattttca agttaccttc tgcatcttga tattcttgaa taatacgacg
     5341 cattactgct ggttgttcat gaatttcttt taacatgtag tgtgcataaa cacctttttc
     5401 agcatctgat gcatcaattt cagcaatata tgaatcacgt tctacaacgt ttccatctgc
     5461 atctttaata ataacttcat cttttttaac aataacgatt tcatggtcat ggatttcttt
     5521 atattcgctt gtcacttgta acattgcaag tgcgtctgat gcgataacat tgaaaccttc
     5581 accaacacct aataataatg gtgatttatt tttagcaaca tagattgtgt ctttgtcttc
     5641 agcatctaat aaacctaatg catatgaacc atgtaataat gacacaactt ttgtaaatgc
     5701 ttcttcagtt gaaagtcctt gatttgaaaa gtattcaact aattgaacga taacttctgt
     5761 atctgtttct gaaatgaatg atacaccttg taagtattca ccttttaact cttcatagtt
     5821 ttcaataaca ccgttatgaa ctagagtaaa acggccattt gatgattgat gtggatgaga
     5881 gttttcatga ttcggtacac cgtgtgttgc ccaacgtgtg tgaccgattc caacaggtcc
     5941 atcaaaatcg ctactatcag caactttacg taattctgca atacgacctt tttctttaaa
     6001 tacagttgta ttatcatcat ttactaccgc gatacctgca gagtcataac ctctgtattc
     6061 taatttttct aaacctttta ataataattc tttggcatta tcatagccaa tataaccaac
     6121 aattccacac ataacgacat tttcctccat attggaatag tacgcgtaaa ttatgattta
     6181 ttgccgataa tttagattga caatctgctt tcataatata aataggaaca tgctattatc
     6241 gcattcatcc ataacaaatt aagcatagtt atttttacaa ctatacaaat tgctcacact
     6301 gtactttcca tattaatatt ttttatattc aatttctggc gatcttatta actttgtcca
     6361 ttaagtcacc ctaatgtttt acttaataag ctaacgaatg agccacatcc gggatagcat
     6421 ccgccgatct attcgatcac tatcctcctc gtctacaaat acatatattg cactctataa
     6481 aggccactca tatattaacc tttaatcttc aaatacaaat atttatttgc acaggcgctt
     6541 taactgtact gccgaacttt ccccctttcc attaatcatt attgtacaac ggtgttgttt
     6601 tgttttgcaa atattttcac aataaaattt taaaaatcct aaaacaattt ttttgtttta
     6661 ttttttcaaa atatctatac tgtcacattg atgacacttt atttaatttt gtcacattta
     6721 ttttgacaaa gttgattttt gtttatattg agtaacaagt aacgtctcta tacactatat
     6781 atagtcacat atattaaaaa agaggtgtaa atatgtcgca aactgaagag aaaaaaggaa
     6841 ttggtcgtcg cgttcaagca ttcggatcat ttttgagtag tatgattatg ccaaacattg
     6901 gtgcattcat cgcttggggt ttcatcgcgg ccatttttat agataatgga tggttaccta
     6961 acaaagattt agctacttta gcaggaccaa tgattactta tttaatccca ttattgatcg
     7021 catttagtgg tggtcgttta atttatgatt tacgtggtgg tatcatcgca gcaacagcaa
     7081 ctatgggggt catcgttgca ttacctgata caccaatgtt acttggtgca atgattatgg
     7141 gtccacttgt tggttggtta atgaagaaga ccgaccaatt gattcaacct agaacgccac
     7201 aaggttttga aatgttattt aataactttt ctgctggtat tttaggattt atcatgacga
     7261 ttgcaggatt taaaatttta gcaccactta tgaaatttat tatgcatatt ttatcagtgg
     7321 cagttgaagc attagtacat gcacacctac ttccacttgt aagtatttta gtagagcctg
     7381 caaaaattgt atttttaaac aatgcgatta accatggtgt attcacaccg cttggtgcag
     7441 atcaagctgc aaaagctggt caatcaattt tatacacaat tgaatctaac cctggaccag
     7501 gtttaggtat cttacttgct tatatgattt ttggaaaagg cactgcaaaa gcaacgtcat
     7561 atggtgctgg aattatccac ttcttaggtg gtattcatga aatttatttc ccatatgtat
     7621 taatgcgtcc tttattattt atcgctgtta ttttaggtgg aatgactggt gttgcaactt
     7681 atcaagcaac aggatttgga tttaaaagtc ccgcgtcacc aggttcattt atagtttact
     7741 gtttaaatgc acctagaggt gaattcttgc acatgttgct cggtgtcttc cttgctgcac
     7801 ttgtatcatt cgttgtagct gctttaatta tgaagttcac tagagaacca aagcaggatt
     7861 tagaagctgc gacagctcaa atggaaaata ctaaagggaa aaaatcaagc gttgcttcta
     7921 agttagtatc ttctgataaa aatgttaata cagaagaaaa tgctagtggt aatgttagtg
     7981 aaacatcttc atcagatgat gatcctgaag cgctattgga taattacaac actgaagatg
     8041 ttgatgcaca caattacaat aatataaatc atgttatttt tgcctgcgat gcgggtatgg
     8101 gttctagtgc aatgggtgca agcatgttac gtaataaatt taaaaaggcg ggcattaatg
     8161 atattacagt tacaaatact gcgattaatc aattgccaaa agatgctcaa ttagttatca
     8221 ctcagaaaaa actaactgat cgtgctatta aacaaacacc aaatgccatc catatttcag
     8281 tggataattt ccttaattca ccaagatatg aagaactttt aaataatcta aaaaaagatg
     8341 atcaagcata ataattaaat aaattaaaaa atggaggata ccgccatgtt attgagtaca
     8401 cgtgaaaaag aaatgatagc cctattgatt aagtaccaca gtcaatatat cactatacac
     8461 gacattgctc agcaacttgc ggtgtcctct cgtactattc accgtgaatt aaaaggtgtt
     8521 gaagcatatt taacttcatt ttcattaact ttagaacgcg caaacaaaaa agggctacgc
     8581 attgctggcg cagattctga tttaaacgat ttgaagcaat cgattgcaca acatcaaacc
     8641 attgacttat ctgttgaaga gcagaaagta attattatat acgctttgat acaagccaag
     8701 gagccagtta aacaatatag tttagcgcaa gaaattggcg tttctgtcca gactttagca
     8761 aagatgttag atgatttaga gcttgattta aataagtacc aactatcttt atctcgaaag
     8821 cgtggcgaag gcatttactt ggtaggtact gaatcaaaga aacgtgaatt tttaagtcaa
     8881 ttaatggtga ataacttaaa tagtactagc gtttattcag taattgaaaa tcattttgtc
     8941 tttcattcat taaatcaaat ccacaaagac tttgttgact tagagcgcat ttttaatgtt
     9001 gaaagactat taatggacta cctaagtgcc ttaccctacc aacttaccga atcaagttat
     9061 ttaactttaa ctgtccatat cgtgctatcc atttcacgta taaaaaatgg agagtatgtc
     9121 gcattaaacg atgatattta tgattctgta caaaacacat ttgaacacaa agtagcaagc
     9181 gaacttgctg ataaacttgg tcaaatatat gacgtcacgt ttaatcaggc agaaattgct
     9241 ttcattacta tccatttacg tggagctaaa cgaaaaaatc ttaatgatac atcattaaat
     9301 aatcgttgtg aagaaaacaa aattaaagcg tttgttaaca aagtagaaat gatttccggt
     9361 atgacatttg cagatttgga tactttagta gatggactga cgctacacct taatcctgca
     9421 atcaatcgtt tgcaagctaa tatcgagacc tataatccgt taacagacat gattaagttc
     9481 aaatatccaa gactatttga aaatgtaaga ttagctttaa atgattgttg gcctgatttg
     9541 atttttccag agagtgaaat tgcttttata gttttacact ttggtggctc gattaaaaac
     9601 caaggtaatc gatttttaaa catattagtc gtttgcagca gtggtatggg aactagtcgt
     9661 ctattatcaa ctcgtctaga gcaagttttt agtgagattg agcgtattac acaagcatca
     9721 gtcagcgatt tgaagtcact agatttaagt caatatgatg gcattatttc tactgtgaat
     9781 ttagacatcg actcccccta tttaacggta aacccattat taccagatag tgatatcagt
     9841 tatgtcgcac agtttttaaa tacaaagtct acgttccaag agacgcatga taaatcatca
     9901 aacatgattg ataaggatga tgttcatgtt gaaacgaaag atgttgatgg caacacatct
     9961 tttgaaaatg aacaaacttc atacttaact tcagttttcg aaaaacattt aagtgacgaa
    10021 aaatcagaac aattattgca tcatatgcgt tcgggtttaa ctttgcttga ttcagtgaaa
    10081 atagttagta ccgaagttaa acagtggcaa acatatatcg cagattatct atatcaatgc
    10141 gatgtaataa acgatccaac gtcattcgct gaactactag agcaacgatt gattgacaat
    10201 ccaggatgga tattaagtcc atatcctgtt gcaataccac acctaagaga caatatgatt
    10261 aaacacccta tgattctaat cacagtttta gaagaaccgt taacattgcc tagtattcaa
    10321 aatgacaatc aaacaattaa atatatgatt tccatgttta ttcctgacaa tgattttatg
    10381 gcatcactgg taagtgactt gtccgaattt ttaagtttga aattagaatc tattgatact
    10441 tttatggaaa atccacagga acttgaaaca ttattaagaa acaaattttt agaacgaatt
    10501 aaaaaacaat ttatttagga gtgtacaaaa tgagcgaatt atttagtaat gacaatatct
    10561 ttttaaatgt aaatgttaac agccaaaatg aagcaattga aaaagcaggt aaagccttag
    10621 ttgatagtgg tgctgtaaca gatgcttata ttcaagcaat gaaagatcgt gagcaagttg
    10681 tatcaacatt tatgggaaat ggcttagcaa ttcctcatgg cacagatgaa gctaaaacaa
    10741 atgtgattca ctcaggttta acattattac aaatccctga aggcgttgac tgggatggcg
    10801 aagtagttaa agttgtagtg ggaatcgctg gtaaagatgg cgaacattta gacttgttat
    10861 ctaaaattgc aattacattt agcgaagaag aaaatgtgga tcgtatcgtt caagcaaaat
    10921 ctgcagaaga aattaaacaa gtattcgagg aggcagatgc ataatgaaag cagttcactt
    10981 tggtgctggt aacataggtc gtggtttcat tggttatatt cttgcagaca acaatgttaa
    11041 agtaacattt gcagacgtca atgaagaaat cattaatgct ttagctcatg atcatcaata
    11101 cgatgttatt ttagctgatg agtctaaaac aacgacgcgc gtgaataatg ttgatgcaat
    11161 taattcaatg caaccttctg aagcgttgaa acaagcaatt ctagaagctg atattattac
    11221 aacagctgtt ggtgttaaca tactacctat tattgctaaa tcttttgcgc ctttcttaaa
    11281 agaaaaaaca aaccatgtta atattgttgc ttgtgagaat gctattatgg caactgatac
    11341 attgaaaaaa gcagtacttg atattactgg ccctcttggt aacaatattc attttgctaa
    11401 ctcagcagtt gatagaattg taccattaca aaagaatgaa aatatattag acgttatggt
    11461 tgagccattt tacgaatggg ttgttgaaaa agatgcatgg tatggtccag aactaaacca
    11521 tattaaatat gttgatgatt taacaccata tattgagcgt aaattattaa ctgtgaatac
    11581 aggacatgca tatttagcgt atgctggtaa atttgcaggt aaagctaccg tattagacgc
    11641 tgtaaaagat agttcaattg aagctggctt acgccgtgtt ttagctgaaa caagtcaata
    11701 tattactaat gaatttgatt ttactgaagc ggaacaagct ggttatgttg aaaaaataat
    11761 agaccgtttc aacaattctt atttatctga tgaagtgaca cgtgtcggac gaggtacgtt
    11821 acgtaaaatt ggccctaaag atagaattat aaaaccatta acatatcttt ataataaaga
    11881 tttagaacgc actggtttat taaatacagc tgcattgtta ttgaagtatg atgatacagc
    11941 agaccaagaa actgttgaga aaaataatta cattaaagaa cacggtttaa aagcgttttt
    12001 aagcgaatat gctaaagttg acgatggctt agccgatgaa ataattgaag cgtacaattc
    12061 actttcataa tttattgagc tttgtttgaa acaagaagtt tccaacgtta ttcgttaaca
    12121 atcagtaata atgtagtagt tccgttgaat taacaatatt aaatttctga acataaaaaa
    12181 tactcccttc aacatagaca cttaacttgt gttatgtatg aaaggagtat ttttgcgtta
    12241 ataatttgtt ttattttcga gccacagcga cctattcaat tgctattggt cattactaaa
    12301 acaaattcat attaactgtt agacttggtt acttagtaag gaatatttcc ctatgaaata
    12361 actagatgtt cacattcttg aataaatttt attcttcagt ttgttggtct ttcttagtga
    12421 atcttctaat taagaatgcc atacctgcac ctagagctaa ttcagcatat ggtaaatcgt
    12481 cattatgtga cataccagta tctggtaaag ttttagcttg ttgtttaggc ttattaactt
    12541 ttccttgttg gtctgatttt gcatttgctt tgttttcttc aggcttagtt acattaagca
    12601 tatcttgatg catactatga ctaccatttg aaactgttgc tggagatgca ttggcaccgt
    12661 cgttttgcgt agctttattg tttgcagctg aaccaactga tttttgcgta tcattagttt
    12721 gcatatcttt agctttattg tttgcaactg ggccaactga tttttgcgta tcattagtat
    12781 ctgctgttgc cgtatcatct ttttggctaa cattagttga agtcatttta tcttttgctt
    12841 cagaagatgc agatgttgat ggtttattcg aaacttcagt atcagctttg cttggcgatt
    12901 tatctgcttc gttagatgca acgttagttt cagacttaag ttgtcctgca tcagtttgat
    12961 ttgtcgtact ttcttcttta tcttttgatg tattagaagg tacatttggt tctgttatgt
    13021 ctgctgaagg caatgtttca gttgttgatt caaccatact ttgatttgtt gaatctctac
    13081 catctttttc tgccttagct ttattttcag attttggttg tgcaaccttg ccattagttg
    13141 attgagattc agcactatta tttacttcag cattttgttt tgaatcattt acagatgcat
    13201 tatctttact atcagcagat gatgctgctt ctgtgctcgc agttgttgga gccgttgctg
    13261 ttgatcctgt tggtgcattc tcgtttgttg ctgtagttgt actattgtta tttgttgtgc
    13321 tttctgctgg cgttgcatta tctgtttctg ttacaggttt atcagttgtg ccgttattag
    13381 ttgattctac ttctggttta ctagttacat cgttatccat tgtcggactg tttgttgatg
    13441 catctgcact agaattgtta ttagcttgcg gtttatcctt tgcatcatca gttgctgatg
    13501 ttactgttgt ttcacctgtt gccgcatcac tattatttgg tgttgtcgga gaagcgtctg
    13561 ctttgccatt atctgtcgtc tcagatacgt taggttgtcc agtgttttct ggtgttgcat
    13621 tagcatttga atttgctgtt gcatcattat tatctgtacc attattagta tcattagcat
    13681 ctggatcatt ctgaggcaca atcgcttcaa ttgcaggtat cgttacattt tgtaattcag
    13741 caacctctgt atttgtttgt gttttatcta atttatcagc aaatctgtca aaatatctac
    13801 ctaaatctgt acgtgcaatt tctttcgccg atgcatctgc atctgcattt ttaattattt
    13861 ctatttgctt gttaaccact tctctaattg cttccaaagc atttttctta actactggat
    13921 taatacgttg tgctttaagt tgttcaagcg cactattttt gacagtagcg atttctgcat
    13981 ttgtagtttg atcagaaata tcttcagttg cttttgataa aatgtcttct aaagcattcg
    14041 taaacgcttc tttttcttca gttgtagcat cagcgtcgac atttacacct gcttcaatct
    14101 ggtctagtgc agtttctaat tcttcgatag ctttttgttt ttctgttgag tcaatttgaa
    14161 tgttatcaaa tgcctcaagt ccttgagctt tcgctttttc aacttcagca gttgttgttg
    14221 catcagtaat accttgttta gcttgatctg taatttgttt aatcattgct aatgcttcag
    14281 ttttttcttc agcagttaac tggtcactat gatcaattga ttctttcgta tcttctgctt
    14341 tagcttcaat agcttggttc gctttaggtt taacagtagc atctacttga atagtatcaa
    14401 ttgctgcttt accttgtgtt aatgcatcat caacgtcacc attatccaca ccattattaa
    14461 tgttttctaa tgcagtttga acattttggt caacttgctt aattgcttgt tgcttttcat
    14521 cttgtgttgc attagtgtca gctgaaatat tatttttctt ctgatctgca taagcatata
    14581 aatctgttgt agctgattct tttttacctg ttggaattgt gtaatcgtta atattatcta
    14641 agtcattatg aatttgaact tcaatgtcat ctttagaagt agcttgatta acattttgat
    14701 ccgcagtttg ttttaattca gcaagttttt gtttcgcttc agcaatttca cttgaagtcg
    14761 atgcgttaga gttatccgct tcgtttactt tagcattata tgcatcttca attttagcta
    14821 aagcatcttt tttgtactca ctaaatgttt taactgcatt aattttagct tttccttctt
    14881 taactgcatt atcaacatat tcatttgttg atgattgatc gacatttgtt ttcgcttgat
    14941 ttaattcagt atcaacttct tgttttgcat cattaatttc ttgttgtgat gcatttggtg
    15001 tttgttctat ttgtgttttc ttatcagctg cagcttgatc taattctttt ttacctgctg
    15061 gtttcttaac aggatttgca tgaagttgtt gaactttttg tactgctgta tctttagcag
    15121 tagttacatc acctgtagta gttgctgcat taatattatt taaaccttct tcatatgctg
    15181 ctctaactgg tccaatatcg ttaccttttt cttcattagt agtctcatta ttattaagta
    15241 tttcagttat tttattttgc atttcagtta gcaattctgc tcttgcattc gttttaacat
    15301 ctgttgatgc ttgaattggg tcaattgctt gaattgcatt atcttttgca gtgtttacat
    15361 catcgattga ctgtgcattt tcaatatttt gattaccttg tgttaattgt gcgtctactt
    15421 gttgatttgc ttgttctttt tcttcagtag tcgcatctgc agtttgtgca ataagcgctt
    15481 tttgttcgtt tgcttttgtt gctaattcat ctttcgcaac atctttaatt gttgtatctg
    15541 cagtaatacc ttgaatatca gcaactgctt gatctttaat ttgcgtaaca tcattagttg
    15601 tttgtgcatt taagatatct tgatacgctt tttctttagc ttttaaaact aaatcttttg
    15661 ctgcattttt ctcttcagtt gtagcgccag ttgtattatc aattgcttga ttttgagttg
    15721 tcacagcttg atcaacttca tttttcgcat tcgatttaac tgctgttgct ggttgtgtgc
    15781 tttgaattga attctttcca gcgtctttcg ctgtatctac accattatca tcagttgcag
    15841 ctgtaatatt atttttcgcg tctgtaactg ctgttgctaa ttgttgaata gctgcttctt
    15901 tttcttcatt tgtagcgttc tgatcattat tgataacatt tgtttgcgtt gcttgtaatt
    15961 gatcaatctc atctttagcc gcttgtttct taacaacttt tggtgttacc gcattaattg
    16021 ctgcttctgc attagctttc gcttcatcaa cttgtgcgtt agtagttgct gctgaaatgg
    16081 cttgatttgc tttaccattt tcagtatttg cttctgcatc agctgcttgt ttttcttcat
    16141 ctgttgcatc tggtgtagct tgaatctctt gcaatttgtt atttaaaatt gctgtgattt
    16201 cattacgtgc agttgctttt ttattaactg ttggtgttac ttgatcaata ctagtttcac
    16261 ctgtcgtttt cgcttggtct acatcatttt gactattagc agcttcaatg ttgctatttg
    16321 cttgtgtcac agcattatct acgtccgcat tagccgctgc aatttcttca gcagtaatat
    16381 cttgcgtttg agcgattgct gttttacgtt cattcgcttt cgtagcaatt gcttgtttcg
    16441 cattatcttt agttgttgtt gctggctgaa tcgcttcaat tttagcaatt tctgcatttt
    16501 tagccgcttc aacttccaca tttgaatgag ctgcatctat tgctgcatca gcagccgttt
    16561 tttcagtttg aacttgttgt ttagcagctt ctttttcttc tgttgttgaa ccgttattag
    16621 catcaattgc tgtttcttga gcttgtactt tatctgcaat tgcttgtttt gctgctggtt
    16681 taacatttgc atctggcgta attgcagcta ttgtagcttc attagtagtt tttgcattat
    16741 ctacatctgt atttgctgta gcattatcta tatcagcgtt tgcagtaact actgcttgat
    16801 caactttatc ttttgcagct tgttgttctt cagtagtcga atcattcatt gcttcaattg
    16861 cagttttacg ttcacttgct ttttgagcga tttccgcttt agcatccgat ttcttagttg
    16921 tggcagcttg cacttgatta attgcagcaa taccattgtc tttagctgtt gttacatcac
    16981 tgtttgtatt tgcagcatca agatttgttc ttgcttcttg ctttttagca tctaattctg
    17041 tatatgcagc ttctttttct tctgtagttg aagcattgct attttggatt tcttgtttac
    17101 gtgtattata tgcgttttct acttctgtat cagctgcagg tttaactttt gcttgtgttt
    17161 gaattgcatt gattttatct aatacttttg ctttagtatc agccacttct tgttgtgatt
    17221 taacaacttg gatgtcatgt aaaccttctg tttgagctgc atctactgct gcatttgcct
    17281 ctgctacttc ttcatcagtt gcatttgaaa ctgtagcatt ttgcgctttt ctagctgtag
    17341 ctgcttgtct aacttcttta taagcatcca ttttagtttg agttgaatcc gtaatattct
    17401 caattttgaa tatttcttcg tttttaacgc gttctaaatc ttgtgcagtc gttgtcgctt
    17461 caattgcttt aacaccagaa actttagctg cattaatacg ttcaatagct tccgctactt
    17521 cttcatttgt tgtatcagga tttaaaggtg cttgatcaat ttgtgcttga acaacttcgt
    17581 caaattcttc aagagctgct gctttagctg ttgtagcagg tgtagtttga ttaatatcat
    17641 tgattgcttt tgttttaatg gcttctactt gtgcatttgt tgttgctgca tcaatatctt
    17701 taattgcctt tgtttcaatt ttaccaattt tatcatttgc tacatctttt tcatcttgta
    17761 atgatgcatt tgacttttta atttgttgtt tacgagttgt aactgcttgg ataatatctt
    17821 gtttcgcttg aggtttaact gttggtgtaa tcacatcttg ttctaacact gcgataccat
    17881 tatctttttc agttgtgaca ccttgtgatg ttgtttgttt attaatttct gcgattgcat
    17941 tatttttatc attgttaatt ttagtcacaa gcgctgcaac ttcatcttgc gtaacttttt
    18001 tactttgttg tgctgctgta accttttctt gagctttagt atctaattca gcaattgctg
    18061 cttgattatc aattaatgca gcttgtaatt tagttactaa accgtcaata tccgcttgag
    18121 atgcacgatt tgcagtttca acgtgattcg catcttcatt taaaatagtg tctgcttgtt
    18181 gtttaagttt attgtattct gcaattgatg ctgttgtgta atgactatta tcaacttgtg
    18241 agtttacttg ttgttgcaac gcatctttgt tcatttcaac cgctactgaa aatggatctg
    18301 cagttagtgt aacttctggt tgcgcattat taattacaac atctgaagca gtacgatatg
    18361 ttaatttttc attaaaatca atatttttag gtgtatcgat attcgcaaca ttaactttat
    18421 atgataattt taaagattta tctgggaata aaacttcttt agtgtgtgta ccacgtgccg
    18481 ttgtcacacc ttggcttgta aatgtaactt tatttgcagt ttgatcataa ttaacagtca
    18541 tatttttcaa tactgtactg tcttcattac cattagggaa tgttgtagtt aatgaatcat
    18601 taacgtaagt tacaccttgt ggtaatgtta cttcatattt aaattgatct gcttttaatg
    18661 aagcaccaaa attaccattg ttaccgattt cagtattaat agtatattct ttattgttag
    18721 ttgctttcgg tgctaaatct ctatttttga catataaatg tgatcctgag aatagaccta
    18781 cattatcaat gaatgaatag tatttataac catctttatt tgttgtaatt cttgaagcat
    18841 cagcaactgc tgtattgtca ggaataaatt gtaaatctaa acgatcagca tcagttggta
    18901 ctttaaataa atgtgcagta ccagtcttag caacatttac agttgctaat tcagtatttt
    18961 tcttagcatc tttaataact aaatttgttg caccttgctt actattcgtt tgcatagtag
    19021 taaagttaat aattaattct gaatctggtt ttacatttac agtttttgta ataccgttaa
    19081 agttaccatg atctgtagaa tcatttgcat tcacacgacc taatgcagcc acgtttcctt
    19141 tagcttgata gttttgaggg ttattcttat caaacatatc gcttcgtctt aattctgagt
    19201 taacgaaacc aatcttaccg ttgttaatta atgaataacc atttacttta tctgtaacag
    19261 ttacagttgg atcctgtcta ttctcatctg ttgatatggc aggatcatca aatgttaatg
    19321 tcgtattaat actgccttca ccagtattgc tagcatttgg atcttgagtt tgtgcgtttg
    19381 ctgctacagg tgctgctggt tgcgctgctg ctggagcatt cgctggctgt gtttgatttg
    19441 ccggtgttgc attattatta ggtgttgctt ggttatttcc ttgacctgct tggtttgccg
    19501 gtgctgcttg atttccaggt tgtgcttgtg cagcgttatt cggatcagct tgattacctt
    19561 gtccagctgg ttgtgctgct cctcctgctg gattagtctg tccaccttgg tttgctggct
    19621 gtgctgcagg attagcttga gcaccagcat ttgcgttagg ctgtgtattg gcatcagctg
    19681 gttgtgctgg ttgattttgt gcaggctgat tttgctctgc tgcagacgct gttgtcgggt
    19741 tagtagatat aaaagtaaca gtggcaatta aagctgaaaa aataccgaca ttaaattttc
    19801 tgatactaaa tttttgttgt ctgaataaat tcattaagtc atcctcctgg ttgattattc
    19861 tcgctgttaa atgatttcac ttaatcaact gttaagataa gtagtagcat ctgcgttaaa
    19921 aacacaaagc aactctatct aattaaaatt aattttatca tcattatata ttgagtacca
    19981 gtgtatttta tattacatat tgattacttt gtttttattt tgtttatatc attttacgtt
    20041 tgtactataa attatttcta gaaacacaaa aaaaccgatg catacgcatc ggcttatttg
    20101 taatacagta tttatttatc taatcccatt ttatcttgaa ccacatcagc tatttgttgt
    20161 gcaaatcttt cagcatcttc atcagttgct gcttcaacca tgacacgaac taatggttct
    20221 gttccagaag gtcttactaa aattcgacct tctccattca tttctacttc tactttagtc
    20281 ataacttctt taacgtcaac attttcttca acacgatatt tatctgttac gcgtacgtta
    20341 attaatgatt gtggatattt tttcatttgt ccagctaatt cacttagtga tttaccagtc
    20401 atttttatta cagaagctaa ttgaatacca gttaataaac catcaccagt tgtattgtaa
    20461 tccatcataa cgatatgccc agattgttct ccacctaagt tataattacc gcgacgcatt
    20521 tcttctacta catatctgtc gccaacttta gttttattag atttaattcc ttcttgttca
    20581 agcgctttgt aaaaacctaa attactcata acagtagaaa caatcatgtc attattcaat
    20641 tcttgatttt tatgcatttc ttgaccaata ataaacataa tttggtcacc gtcaacgatt
    20701 tgaccattct catctactgc tatgattctg tctccatcgc cgtcaaatgc taacccaaaa
    20761 tcactttcag tttcaactac tttttcagct aatttttcag gatgtgtaga gccacatttc
    20821 tcattgatat tatatccatc aggactacat ccaattgttt cagtatctgc ttctaaatca
    20881 ccaaataaga atggcgctag tgatgatgtt gaaccatttg caccatctaa aacaattttc
    20941 aaaccttcaa agttaacatc tactgttgat tttaaatagc tcaaatattt ttgtgcccct
    21001 tcaaagtaat ctgaataatg tacaatatca ttgccaactg gtcttggtaa ttctggattt
    21061 tcttgatcca ataatgcttc aatttcattt tcttgttcat ctgatagttt aaaaccatct
    21121 gatccaaaga atttaatacc attatctgca actggattat gagaggctga aatcattaca
    21181 cctaactctg cacccatatc gcgtgttaaa tatgcaacac ctggtgttga aataatacct
    21241 aatcgcatca cttctgcacc aattgaaatc aaaccagcta ttaatgctga ttctaacatt
    21301 tcacctgaaa ctctagtatc gcgacctaca agtacacgtg ggtgtttttc acctttatta
    21361 tgtgctagaa catagccacc gtatcttcct aatttaaatg ccaattcagg tgttagttct
    21421 tggtttgcga cacctcttac tccgtctgta ccaaaatatt ttcccattgt ttactctcct
    21481 ttaatttagc tatttatttt acatttatat aagccttcgt ttcacttggt tgtgctttag
    21541 tgacatgttc tggtaaattg atttttacag tcttttcagt tgattctgaa ataccatcta
    21601 aatctacttc tgcatcaact tcgcttatat tttgtaagtc atctcgacta ccgaagattt
    21661 caatttcttt atcttctaaa tcaatcgaac ttaactcttt atcatctgct aaactacctt
    21721 tctgtttaac atttacttta acctttttgc taaaaggctc tactttaact tgtaaattca
    21781 cttcattagg ttgaatcgat acatttaatt tattcagttt tttatcaaaa gccgttactt
    21841 ctgcgacatc ttttgtgtca ccattaatct ttttattagt tttaaaagtg gcttttaaat
    21901 aagcgatatc attcaattgt tcttctccac ctgttacttt aactgtttgt ggtgaaactt
    21961 cttgctttgt aattttataa agtggatcaa tatcactctg acttacatca ggttgaactg
    22021 tcatcttttt agttactttg ttttcaagcg taatatttgc taatttaggt tttacagaat
    22081 aatgaatgtc atcagctaac cctttaactt gatacttctc ttgatatttt ccagctttag
    22141 catttgataa atcaatcact actcttaaat cttctggatt ttcaattttt attatctttg
    22201 attgtggtcc tgaaatagtc acattaactg tttcaggcgc ttttgttaaa tgcaaatctt
    22261 tagtgttata aagaatttca acgggtacat cttgaatcgt tttactagac ttttgaccaa
    22321 gattaccagt gttaaagata tttccaaaaa cattattaac agataaaaag aaaaacaatg
    22381 ccaaaagaaa ggcaataaat ctcaagcccc atttactttc tagcatatta tttcacacct
    22441 ttcttttgaa agcgtgtgcc aaaccaatgt tcagcaagca actcttcaaa aatttcgttt
    22501 gaaatgtctc gtcgtaattt tccatcaaat gttaccgaaa tatcaccagt ttcttcagat
    22561 acaataacgg taaatgcatc agatacttct gaaataccaa ccgcagctct atgtcttgta
    22621 cccaaacttt tagatatctt aggactatca gacaatggca aataacttgc tgctgctgca
    22681 atcttagtgc cttgaataat cattgcacca tcatgtaaag gtgtgttagg tataaagaca
    22741 ttaattaaaa gttcttgcga aatatttgaa tccattgcaa tacctgtttc aatataatct
    22801 tgaagacctg tttctttttc aaagacaatt aatgcaccta tacgtctttt agccatatat
    22861 tgcacagcct ttgaaaccga ttgaatcaat ttctcttcat ctttactata cgtattagaa
    22921 gtatagcgtt ttaaaaagct acctctacca agttgttcta acgcacgtct aatttctggt
    22981 tggaatatta ctattaaagc taataccccc cattgaataa cgatatcgaa taatttagat
    23041 gttgcagtca agttcaatat catacttatc tgctgaccaa taacaattac taatatccct
    23101 ttaagtaatt gtatcgcttt agttccctta aagaccgtga tgagaagata aagtacatac
    23161 caaactatca gtaaatcaag gatactcgtt acaattttta acgtactgag gttttgaaaa
    23221 aagttggaaa aatccataac atctcctccg ggtaatattt ttccataata cccattatac
    23281 caatcattta catcactgtc atacgaacat atattta
//
LOCUS       DANBWH010000016        22945 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.016, whole genome shotgun sequence.
ACCESSION   DANBWH010000016 DANBWH010000000
VERSION     DANBWH010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 22945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22945
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.016"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..539
                     /locus_tag="PPY92_000434"
     CDS             <1..539
                     /locus_tag="PPY92_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624458.1"
                     /translation="GFNLYRHQMQHLSLSAMLLSITINAFINTFIIDKYNFITLCTIT
                     MFIIWTILQFYIDKKLKPVYITDQKFIAIILTIVVSLTQRVTDFSSTQSIYMSIPFLA
                     PAIFIIGGIMLFISTFNSLDETAENNNKIKKLMIKGLIIINISFIVMMVLTPYWYLYL
                     IVYLIFLLFLLWQKVYKF"
     gene            819..1259
                     /locus_tag="PPY92_000435"
     CDS             819..1259
                     /locus_tag="PPY92_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HDJ2624459.1"
                     /translation="MNTKYFLAVGAVASVLTLGACGNSNSQDQGNKTEQKTKSEDSNV
                     KTDKTKHLTGTFSSKNGETVEGKAEIKNGKLMLTNYKSSKGPDLYVYLTKNGDIKNGK
                     EIAMVDYDKEKQTFDLKNVDLSKYDEVTIYCKKAHVIFGGAKLK"
     gene            1602..2075
                     /locus_tag="PPY92_000436"
     CDS             1602..2075
                     /locus_tag="PPY92_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDJ2624460.1"
                     /translation="MNKLLLLVTFIIRVGSGIVMLMQGYEKLTGGFTLKGLVPVIANN
                     TDSPEWYKWFFANIVAHTTSLFDIVVPLGEIAIGLGLIFGVFAYAASFFGAFVMINYI
                     LADMIFTYPLQLTFFILLLMSHSLLKQISLKEIINYFRGRKNRGEKIDDPLTDRG"
     gene            2050..2736
                     /locus_tag="PPY92_000437"
     CDS             2050..2736
                     /locus_tag="PPY92_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDJ2624461.1"
                     /translation="MTHLLIVDDEQDIVDICQTYFEYEGYKVTTTTSGKEAISLLSND
                     IDIMVLDIMMPEVNGYDIVKEMKRQKLDIPFIYLTAKTQEHDTIYALTLGADDYVKKP
                     FSPRELVLRINNLLTRMKKYHHQPVEQLSFDELTLINLSKVVTVNGHEVPMRIKEFEL
                     LWYLASRENEVISKSELLEKVWGYDYYEDANTVNVHIHRIREKLEKESFTTYTITTVW
                     GLGYKFERSR"
     gene            2736..3791
                     /gene="saeS"
                     /locus_tag="PPY92_000438"
     CDS             2736..3791
                     /gene="saeS"
                     /locus_tag="PPY92_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SaeS"
                     /protein_id="HDJ2624462.1"
                     /translation="MVLSIRSQIIIGVVSSILLTSTILAIAYILMWFNGHMTLTLTLT
                     TIITSCLTLLICSIFINPLIQKIKQFNIKTKQFANGNYASNDKTFNSPKEIYELNQSF
                     NKMASEITQQMNQIKSEQQEKTELIQNLAHDLKTPLASIISYSEGLRDGIITKDHEIK
                     ESYDILIKQANRLSTLFDDMTHIITLNTGKTYPPELIQLDQLLVSILQPYEQRIKHEN
                     RTLEVNFCSEIDAFYQYRTPLERILTNLLDNALKFSNVGSRIDINISENKDQDTIDIA
                     ISDEGIGIIPELQERIFERTFRVENSRNTKTGGSGLGLYIANELAQQNNAKISVSSDI
                     DVGTTMTVTLHKLDITS"
     gene            complement(3863..4846)
                     /gene="csbB"
                     /locus_tag="PPY92_000439"
     CDS             complement(3863..4846)
                     /gene="csbB"
                     /locus_tag="PPY92_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosylation protein
                     CsbB"
                     /protein_id="HDJ2624463.1"
                     /translation="MKIRVVIPCFNEGEVITQTHQQLTEILSQDSSVKGYDYNMLFID
                     DGSTDTTIDEMQHLATIDRHVSFISFSRNFGKEAAMIAGYQHSTEFDAVIMIDCDLQH
                     PPEYIPKMVEGFMEGYDQVIAKRDRSGENFSRKTLSHLYYKLVNCFVEEVQFDDGVGD
                     FRLLSQRAVKSIASLEEYNRFSKGLFEWIGYNTKVFTYQNVERQKGESKWSFKKLFNY
                     GIDGLISFNSKPLRMMIYLGLFIFSISALYIIYLFINIMISGVNIPGYFSTIAAILLL
                     GGIQLISIGVVGEYIGRIYYEVKARPKYIIQATNLSSIENDEKDTHKVYSK"
     gene            complement(4978..5817)
                     /locus_tag="PPY92_000440"
     CDS             complement(4978..5817)
                     /locus_tag="PPY92_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDJ2624464.1"
                     /translation="MLNEIQILNNGYPMPSVGLGVYKISDEDMTKVVNAAIDAGYRAF
                     DTAYFYDNEASLGRALKDNGVDREDLFITTKLWNDYQGYEKTFEYFNKSIENLQTDYL
                     DLFLIHWPCEADGLFLETYKAMEELYEQGKVKAIGVCNFNVHHLEKLMAQSSIKPMVN
                     QIEVHPYFNQQELQEFCDRHDIKVTAWMPLMRNRGLLDNPVIVKIAEKYHKTPAQVVL
                     RWHLAHNRIIIPKSQTPKRIQENIDILDFNLELTEVAEIDALDRNARQGKNPDDVKIG
                     DLK"
     gene            complement(6039..7388)
                     /locus_tag="PPY92_000441"
     CDS             complement(6039..7388)
                     /locus_tag="PPY92_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDJ2624465.1"
                     /translation="METSTIISLIIFILLIALTTVFVGSEFALVKIRATRIEQLADEG
                     NKPAKIVKKMIANLDYYLSACQLGITVTSLGLGWLGEPTFEKLLHPIFEAINLPTALT
                     TTISFAVSFIIVTYLHVVLGELAPKSIAIQHTEKLALVYARPLFYFGNIMKPLIWLMN
                     GSARVIIRMFGVNPDAQTDAMSEEEIKIIINNSYNGGEINQTELAYMQNIFSFDERHA
                     KDIMVPRTQMITLNEPFNVDELLETIKEHQFTRYPITDDGDKDHIKGFINVKEFLTEY
                     ASGKTIKIANYIHELPMISETTRISDALIRMQREHVHMSLIIDEYGGTAGILTMEDIL
                     EEIVGEIRDEFDDDEVNDIVKIDNKTFQVNGRVLLDDLTEEFGIEFDDSEDIDTIGGW
                     LQSRNTNLQKDDYVDTTYDRWVVSEIDNHQIIWVILNYEFNEARPTIGQSDEDEKSE"
     gene            complement(7609..8790)
                     /gene="nagA"
                     /locus_tag="PPY92_000442"
     CDS             complement(7609..8790)
                     /gene="nagA"
                     /locus_tag="PPY92_000442"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDJ2624466.1"
                     /translation="MSELIIYNGKVYTEDGKIDNGYIHVKDGQIVAIGEGDDKAAIDN
                     DTTNKIQVIDAKGHHVLPGFIDIHIHGGYGQDAMDGSYDGLKYLSENLLSEGTTSYLA
                     TTMTQSTDKIDKALTNIAKYEAEQDVHNAAEIVGIHLEGPFISENKVGAQHPQYVVRP
                     FIDKIKHFQETANRLIKIMTFAPEVEGAKEALETYKDDIIFSIGHTVATYEEAVEAVE
                     RGAKHVTHLYNAATPFQHREPGVFGAAWLNDALHTEMIVDGTHSHPASVAIAYRMKGN
                     ERFYLITDAMRAKGMPEGEYDLGGQKVTVQSQQARLANGALAGSILKMNHGLRNLISF
                     TGDTLDHLWRVTSLNQAIALGIDDRKGSIKVNKDADLVILDDDMNVKSTIKQGKVHTF
                     S"
     gene            complement(9098..11056)
                     /locus_tag="PPY92_000443"
     CDS             complement(9098..11056)
                     /locus_tag="PPY92_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDJ2624467.1"
                     /translation="MRVTELLTKDTIAMDLMANDKNGVIDELVNQLDKAGKLSDVASF
                     KEAIHNRESQSTTGIGEGIAIPHAKVAAVKSPAIAFGKSKEGVDYQSLDMQPAHLFFM
                     IAAPEGGAQTHLDALAKLSGILMDENVREKLLHASSPEEVLAIIDEADDEVTKEEEAE
                     AEAQQVATAEQSSKQSNEPYVLAVTACPTGIAHTYMARDALKKQADKMGIKIKVETNG
                     SSGIKNHLTEQDIENATGIIVAADVHVETDRFDGKNVVEVPVADGIKRPEELINKALD
                     TSRKPFVARDGQRKGNSNDSQEKLSPGKAFYKHLMNGVSNMLPLVISGGILMAIVFLF
                     GANSFNPKSSEYNAFAEQLWNIGSKSAFALIIPILSGFIARSIADKPGFASGLVGGML
                     AISGGSGFIGGIIAGFLAGYLTQGVKAMTRKLPQALEGLKPTLIYPLLTVTATGLLMI
                     YVFNPPASWLNHLLLDGLNNLSGSNIVLLGLVIGAMMAIDMGGPFNKAAYVFATGALI
                     EGNAAPITAAMIGGMIPPLAIATAMLIFRRKFTKEQRGSIIPNYVMGMSFITEGAIPF
                     AAADPLRVIPSMMIGSGIGGAIALGLGSRITAPHGGIIVIVGTDGAHLLQTLIALLVG
                     TLVSALIYGLIKPKLTETEIEASKSMDE"
     gene            complement(11062..11982)
                     /gene="pfkB"
                     /locus_tag="PPY92_000444"
     CDS             complement(11062..11982)
                     /gene="pfkB"
                     /locus_tag="PPY92_000444"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDJ2624468.1"
                     /translation="MIYTVTFNPSIDYVIFTNDFKIDGLNRATATYKFAGGKGINVSR
                     VLKTLDVESTALGFAGGFPGKFIIDTLNNSAIQSNFIEVDEDTRINVKLKTGQETEIN
                     APGPHITSTQFEQLLQQIKNTTSEDIVIVAGSVPSSIPSDAYAQIAQITAQTGAKLVV
                     DAEKELAESVLPFHPLFIKPNKDELEVMFNTTVNSDTDVIKYGRLLVDKGAQSVIVSL
                     GGDGAIYIDKEISIKAVNPQGKVVNTVGSGDSTVAGMVAGIASGLTIEKAFQQAVACG
                     TATAFDEDLATRDAIEKIKSQVTISVLDGE"
     gene            complement(11979..12743)
                     /locus_tag="PPY92_000445"
     CDS             complement(11979..12743)
                     /locus_tag="PPY92_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDJ2624469.1"
                     /translation="MMIITEKRHELILEELSHKDFLTLQELIDRTGCSASTIRRDLSK
                     LQQLGKLQRVHGGAMLKENRMIEANLTEKLATNLDEKKMIAKIAANQINDNECLFIDA
                     GSSTLELIKYIQAKDIIVVTNGLTHVEALLKKGIKTIMLGGQVKENTLATIGSSAMEI
                     LRRYCFDKAFIGMNGLDIELGLTTPDEQEALVKQTAMSLANQSFVLIDHSKFNKVYFA
                     RVPLLESTTIITSEKALNQESLKEYQQKYHFIGGTL"
     gene            complement(12993..13475)
                     /gene="ybaK"
                     /locus_tag="PPY92_000446"
     CDS             complement(12993..13475)
                     /gene="ybaK"
                     /locus_tag="PPY92_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDJ2624470.1"
                     /translation="MAKNKKTNAMRMLDRAKIKYEVHSFEVPEEHLSGAEVAELIQAN
                     VKTVFKTLVLENTKHEHFVFVIPVSETLDMKKAAALVGEKKLQLMPLDNLKNVTGYIR
                     GGCSPVGMKTLFPTVVDKSCENYSHISVSGGLRTMQITIAVEDLITITKGKIGAVIHE
                     "
     gene            complement(13649..14101)
                     /locus_tag="PPY92_000447"
     CDS             complement(13649..14101)
                     /locus_tag="PPY92_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624471.1"
                     /translation="MTVKNLFLGFVAVILTVCLIGLLILATNEDALAKVHKTINTLNA
                     INVSTEDTYKKKMDILNIHTAKASEVNENVEKQNHFKHRVNANKSNSFNEQECQVIAD
                     RYADKHINDNYGLERISKTNHGYNYVYSNDNSTSKQHVSISNQGIITK"
     gene            complement(14394..15560)
                     /gene="norA"
                     /locus_tag="PPY92_000448"
     CDS             complement(14394..15560)
                     /gene="norA"
                     /locus_tag="PPY92_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorA"
                     /protein_id="HDJ2624472.1"
                     /translation="MNKQIFVLYFNIFLIFLGIGLVIPVLPVYLKDLGLTGSDLGLLV
                     AAFALSQMIISPFGGTLADKLGKKLIICIGLILFSVSEFMFAVGHNFSVLMLSRVIGG
                     MSAGMVMPGVTGLIADISPSHQKAKNFGYMSAIINSGFILGPGIGGFMAEVSHRMPFY
                     FAGALGILAFIMSIVLIHDPKKSTTSGFQKLEPQLLTKINWKVFITPVILTLVLSFGL
                     SAFETLYSLYTADKVNYSPKDISIAITGGGIFGALFQIYFFDKFMKYFSELTFIAWSL
                     LYSVVVLILLVFANGYWSIMLISFVVFIGFDMIRPAITNYFSNIAGERQGFAGGLNST
                     FTSMGNFIGPLIAGALFDVHIEAPIYMAIGVSLAGVVIVLIEKQHRAKLKEQNM"
     gene            15769..16191
                     /locus_tag="PPY92_000449"
     CDS             15769..16191
                     /locus_tag="PPY92_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDJ2624473.1"
                     /translation="MKLQKSNHTILLVLEKGEDIVECITTFADDQDLTFTSVSGIGAC
                     DDVVLKFFNLTTKQYEEKHITEPLELTSLLGNISRLDNGHFAHLHATFGTQSYETFSG
                     HLAKAIVSATAEIILTVTDLDIQRSFKDAVGLNLLDPQ"
     gene            16377..16994
                     /locus_tag="PPY92_000450"
     CDS             16377..16994
                     /locus_tag="PPY92_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HDJ2624474.1"
                     /translation="MQSRYIARIYFLILFIVSLFETKIFQFMTLNLFLAYIPFELSLL
                     LRLFKPNKKYEWPLFIIYSFIFVLLLPNTFYMVTDLIHLNQFQFNFYAGLNLSEWKYF
                     TYLLLGVFLAVYVMILIYFEILTLTSHRWLNRLIVVVLMFLNGFGIYIGRFLRLHSVY
                     FFNEPLHVLNHILESLTLKTAMFVCFMVVMQAAILLFGKGVRLNK"
     gene            16991..17275
                     /locus_tag="PPY92_000451"
     CDS             16991..17275
                     /locus_tag="PPY92_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624475.1"
                     /translation="MTINIIVLIVSIIVFQLIIGHIWHDIGLSYLRSILLMMLPFGLG
                     VFIQQVSYYERQYPKWQVPQNIKVRLKYIYLATFLEYVVLYLTLFTDILR"
     gene            complement(17430..18803)
                     /locus_tag="PPY92_000452"
     CDS             complement(17430..18803)
                     /locus_tag="PPY92_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HDJ2624476.1"
                     /translation="MAIAVLLNRMFRMEHNPLFEYIYQQKEDIDACYFIIPEEDMSSA
                     SDLKAQFYRGTLQRFYQSLHAEKLTPYVMSYDDIISFCKENNISEVVIAGDIMSYHLE
                     EYDILHQRSLFNEARIAVTLIRGNHYFKASKTMNQQGEPYKVFTSFYRKWRPYLRHRD
                     VYHYDLKSFEDFVIASPDDLVFDDIAFGSSQVIEQNKWQHFLDQDIQNYESGRDYLPE
                     VLTSQLSVALAYGLLDIIEIFNDLLARYDEDEANHEAFIRELIFREFYYVLMTQYPET
                     SYQAFKPKYRQIKWSQNAANFNAWCEGQTGFPIIDAAIMELTQTGFMHNRMRMVVSQF
                     LTKDLFIDWTWGEKFFRKHLIDYDAASNIHGWQWSASTGTDAVPYFRMFNPIRQSERF
                     DAKALYIKTYLPIFNQIDAKYLHDTQRNESNLFEQGIELGSHYPRQMVDHQEKRTQVL
                     ATFKALD"
     gene            complement(18889..20442)
                     /locus_tag="PPY92_000453"
     CDS             complement(18889..20442)
                     /locus_tag="PPY92_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HDJ2624477.1"
                     /translation="MSEEKHVVEHEQQKKEKTKKQYKPFWIVMSFIILIVVLLLPAPS
                     SLPIMAKAVLAILAFAVIMWVTEAVSYPVSATLIIGLMILLLGFSPVQNLGEKLGNPK
                     SGSAILAGSDLLGTNHALSLAFSGYATSAVALVAAALFLAAAMQETNLHKRLALLVLS
                     IVGNKTRNIVIGAIIVSIVLAFFVPSATARAGAVVPILLGMIAAFKVSKDSKLASLLI
                     ITSVQAVSIWNIGIKTAAAQNIVAINFINHQLGFDVSWGEWFLYAAPWSIVMSVALYF
                     IMIKVMPPEINTIEGGKDLIKEELHKLGPVSPREWRLIVISMLLLLFWSTEKVLHPID
                     SASITIIALGVMLMPKIGVMTWKHVENKIPWGTIIVFGVGISLGNVLLKTGAAQWLSD
                     QTFGVLGLKHLPIIATIALITLFNILIHLGFASATSLSSALIPVFISLTSTLHLGDQS
                     IGFVLIQQFVISFGFLLPVSAPQNMLAYGTGTFTVKDFLKAGIPLTIVGYILVIVFSM
                     TYWKWLGLL"
     gene            complement(20725..21012)
                     /locus_tag="PPY92_000454"
     CDS             complement(20725..21012)
                     /locus_tag="PPY92_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624478.1"
                     /translation="MTNEDKRFEQLRFERKFIVIPYLIYAVIVLLLNIFYSDLKITMT
                     LFGLFFAYNVVILFIAFIKHYKRTLLLSLILTVLSGAAFFGIIYVYGINHF"
     gene            complement(21047..21955)
                     /locus_tag="PPY92_000455"
     CDS             complement(21047..21955)
                     /locus_tag="PPY92_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HDJ2624479.1"
                     /translation="MEQIMINHYVHFSRLVQGFWRANEWKMTAKELNYFINELVERGI
                     TTMDHADIYGDYQCESLFGNALDLSPELRNKIQIVTKCGIILPSKQFDFTNGHRYDLS
                     SKHIVKSVEQSLINLNVDYLDSLLIHRPSPLMDPEQVADALTKLVKQGKLKSFGVSNF
                     NHSQYQLLNQYIMKERLHISINQLELSPYHVDSLQDGTMDSMYQNHVQIMAWSPFAGG
                     KIFDKEDIKAQRIMKVVQSIADKYGVSDTAVMIAWLVKIPHSIMPILGTSQLKRIDQA
                     IEGLQLNLDDQSWFDIYTAIIGQDIP"
     gene            complement(22058..>22945)
                     /locus_tag="PPY92_000456"
     CDS             complement(22058..>22945)
                     /locus_tag="PPY92_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDJ2624480.1"
                     /translation="INGFLGSGKTTLLTHYISELLKNDEKIKIIMNEFGTFDIDSNSI
                     SNEIEVHSLINGCVCCDLKQELVYELKAIALKGDVNHVIIEATGIAHPLELLVACQDP
                     QIVNFFEKPIIYGVLDATRFLERHQYTENTVSLMEEQLKLSDMIIINKIDLITDDSLE
                     KIDKQLGMICASIPTYKTTYGKVSLEELDLTVKDREISSHHHHHHGIKSMTYTFTGPI
                     DRQLFYQFIMKLPESVLRLKGYVSFRNQPNAIYEFQYAYGLPDYGIIGMQLPLTIVII
                     GETLDTNHIRNQLDMLQFT"
BASE COUNT         7636 a         4189 c         3184 g         7936 t
ORIGIN      
        1 ctggattcaa tttatatagg catcaaatgc aacatttatc attaagtgca atgttgttat
       61 ctattacgat aaacgccttt ataaatacat tcataattga taaatataac tttattactc
      121 tgtgtactat aacgatgttt atcatttgga cgatactaca gttttacatt gataaaaaat
      181 taaaacctgt atacattaca gaccaaaaat ttattgctat catattaacg attgttgtca
      241 gtttaacgca acgtgttacc gacttttcaa gtacacaatc aatatatatg tctattccat
      301 ttttagcccc tgcaattttc atcattggtg gtattatgtt gtttatttca acatttaata
      361 gcttagatga aactgctgaa aataataaca aaataaagaa actaatgatt aaaggactta
      421 tcattattaa catttcattt atcgttatga tggttttaac accatattgg tacttgtatt
      481 taatcgtcta tcttattttc ttgttgtttt tattgtggca aaaggtttat aaattttaat
      541 accaaaacta taaaacactt ctgatattct tagttcaaaa tatcagaagt gttttatagt
      601 gttatctagt tcagataaat attttctcac ctaaaaaaat gccctcctct tattttgact
      661 cctatttatt taaatcagac aattattttc attttcaaat tattctttct tcaatattag
      721 ttaagcgata tttaaacgaa gttaagaatt agttaatggc atattatttg ccttcatttt
      781 aaacttaact tatcaaattg aagaaatgag gagttagcat gaatacaaaa tattttttag
      841 cagttggtgc tgttgcctct gtattaactt taggcgcttg tggtaattct aattcacaag
      901 atcaaggtaa caaaactgaa caaaaaacaa agtcagaaga tagcaatgtt aaaactgata
      961 aaacaaaaca cctaacaggt acattcagtt ctaaaaacgg tgaaactgtt gaaggtaaag
     1021 ctgagattaa aaatggtaaa ttaatgctta ctaactacaa atcatcaaaa ggtccagatt
     1081 tatacgtcta cctaacaaaa aatggcgaca ttaaaaacgg taaagaaatc gcaatggttg
     1141 actacgataa agaaaaacaa acatttgatc ttaaaaatgt agatttaagc aaatatgatg
     1201 aagtaacaat ctattgtaaa aaagctcacg tcatcttcgg cggcgctaaa ttaaaataat
     1261 caatttctga gttaaacttt tatatacaac atactattac tatacattac aaattttaaa
     1321 aatatacatt acactcatta ctcaatggaa agcgtatgat ttcacagccc ccctagcttg
     1381 tagaaatcat actttccttt tttcaatata tatacaacta ttaaatccca taagattgta
     1441 gagcacataa gtaaaatttt tttagagctt gaggtttgtt tagcttaagc aacccatgag
     1501 ctcaaacact tcctgttcac ataacactac aaatcgcatt atgttgctta atcttatgtt
     1561 tatataaatt acacacaata aatagaaaga atgtgaacat catgaataaa ttattgctac
     1621 tcgttacatt tatcattcgt gtgggttcag gtattgttat gttaatgcaa ggctacgaaa
     1681 aattaacggg cggatttacg ctgaaaggtt tagtaccagt catcgctaac aatactgatt
     1741 caccagagtg gtataagtgg tttttcgcaa atatagttgc acatacgacg tcattatttg
     1801 atattgttgt cccacttgga gagattgcaa ttggattagg tttaattttt ggagtttttg
     1861 catatgctgc tagtttcttt ggagcctttg ttatgataaa ttatatctta gcagatatga
     1921 tatttacgta tcctcttcaa ttaactttct ttatcctttt actaatgagt cactcattgt
     1981 taaaacagat ttcacttaaa gaaatcatta attactttag aggtcgtaag aacagaggtg
     2041 aaaaaataga tgacccactt actgatcgtg gatgatgaac aagacattgt agacatttgt
     2101 caaacctatt ttgaatatga aggttacaaa gtaacaacga caactagcgg taaagaagca
     2161 atttctttac tatcaaatga tattgatatc atggtacttg atatcatgat gccagaagtt
     2221 aatggttacg acattgtcaa agaaatgaaa aggcaaaaat tagatatccc ctttatctat
     2281 ttaactgcca aaacacaaga acatgatacc atttacgcct taactttagg tgcagatgac
     2341 tatgtcaaaa aaccatttag tccaagggaa ctcgttttac gtattaataa tttacttaca
     2401 agaatgaaga aataccatca tcaaccagtt gaacaactgt cgtttgatga attaacactt
     2461 attaacttaa gtaaagttgt gactgtaaat ggtcacgaag tccctatgcg tattaaggaa
     2521 tttgagttat tgtggtattt agcttctaga gaaaatgaag ttatttctaa atcagaatta
     2581 cttgaaaaag tttggggata tgactattac gaagatgcta ataccgtgaa tgtccatata
     2641 caccgtatta gagaaaaatt agaaaaagag agctttacaa catataccat cacaactgta
     2701 tggggattag gatataaatt tgaaaggagc cgataatggt gttatcaatt agaagtcaaa
     2761 tcattattgg cgtcgtttcg agtatactat taacttcaac tattttagca attgcatata
     2821 ttttaatgtg gtttaacggc catatgacac taactttgac cttaacgaca ataattacaa
     2881 gctgtttaac cttattaata tgtagtattt ttattaatcc acttatacaa aaaattaagc
     2941 agtttaatat aaaaactaag caatttgcta acggaaatta cgcaagcaat gataaaacgt
     3001 ttaattcacc aaaagaaatt tatgaattaa atcaatcttt taataaaatg gcttctgaaa
     3061 ttacgcaaca aatgaatcaa attaaatccg aacaacaaga aaaaacagaa ctgattcaaa
     3121 acttagccca tgatttaaaa acacctttag caagcattat ttcatattct gaaggactac
     3181 gtgatggtat aatcacaaag gatcatgaga ttaaagagtc atacgacata ttaattaaac
     3241 aagcaaacag attatcaaca ttatttgatg atatgactca tattatcact ttaaatacag
     3301 gtaaaacata tcccccagaa ttaatacaac tagaccaatt acttgtatca atattgcaac
     3361 catatgagca acgtatcaaa catgaaaacc gcacattaga agtgaatttc tgtagcgaaa
     3421 ttgatgcatt ttatcaatat cgaacgccac ttgagcgtat tttaacaaac ttacttgata
     3481 atgcgctaaa attttcaaat gttggtagtc gcattgatat taatattagt gaaaacaaag
     3541 atcaagatac tatcgacatt gctattagcg atgaaggtat tggcattata ccagaactac
     3601 aagaacgtat attcgaacgt acattcagag tagaaaactc tcgtaataca aaaacgggtg
     3661 gttctggatt aggcttatat atagctaatg aactcgcgca acaaaataac gcaaaaatca
     3721 gtgtaagcag tgatatagat gtaggaacta cgatgactgt aacattacac aaattagaca
     3781 ttacgtcata atccgattta tttataaaat aaaatgcaaa gactaaaaag aagctcccat
     3841 taatgagggc ttcttttttt gtttatttag aataaacttt atgggtatcc ttctcatcat
     3901 tttcaatact tgaaagattt gtagcttgaa taatatattt aggacgtgcc ttaacttcat
     3961 aatatatcct gccaatatat tcacctacaa caccaattga aattaactgt atgccgccta
     4021 ataataaaat agctgcaatc gttgaaaaat atcctggaat attaacacca gatatcataa
     4081 tattgatgaa taaatagata atatatagcg cgcttattga aaagataaac aagccaagat
     4141 aaatcatcat tctcaaaggt ttactattaa aggaaatcaa tccatcaata ccataattaa
     4201 ataacttttt aaaggaccac ttagattccc ctttttgtct ctcaacattt tgatacgtaa
     4261 acactttagt attatagcct atccattcaa ataacccttt tgaaaatcga ttatattctt
     4321 caagtgatgc aatggattta acagctcttt ggcttaaaag tctaaaatca ccaacaccat
     4381 catcaaattg tacttcttct acaaagcaat taactaactt ataatacaaa tggcttaatg
     4441 ttttgcgact aaaattttca ccacttctat cacgctttgc aatcacttga tcatagcctt
     4501 ccataaaacc ttcaaccatt ttcggaatat attcaggtgg atgttgcaaa tcacaatcta
     4561 tcatgatgac tgcatcaaat tcagtactat gctggtaacc tgcaatcata gctgcttctt
     4621 ttccaaaatt tctactaaaa gaaataaagc tgacatgcct atctattgtg gcaagatgtt
     4681 gcatttcatc tatagtggta tccgtacttc catcatctat gaaaagcata ttataatcat
     4741 agcctttcac actactatct tgtgaaagta tttcagttaa ttgttgatgt gtttgtgtaa
     4801 tgacttcccc ttcattaaaa caaggaatga cgactctaat tttcatctat gtccatcctt
     4861 tttatttaaa agattttgaa tgtacctgag tattgtttta tcaaatagta aaaaccaact
     4921 catttatttt aatgtacaca ttaaaaggca ttcataaacg taaaatttaa catccagtta
     4981 ttttaaatcc ccaattttca catcatctgg atttttacct tgtcttgcat ttctatctaa
     5041 agcatcaatt tcagctactt ctgttaattc taaattaaaa tctaaaatat ctatattttc
     5101 ttgaatgcgt ttaggtgtct gagattttgg aataataatt ctattgtgtg ctaaatgcca
     5161 acgtaataca acttgtgctg gtgttttatg atatttttca gcaattttaa caatgacagg
     5221 gttgtctagt agtcctctat ttctcatcaa aggcatccat gcagtcactt taatatcgtg
     5281 acgatcacaa aattcttgta attcttgttg gttaaaatat ggatgtacct caatttgatt
     5341 caccattggt ttgatacttg attgagccat taatttttct agatgatgaa cattaaaatt
     5401 acatacacct attgctttta ccttaccttg ctcgtaaagt tcttccatag ctttatatgt
     5461 ttctaaaaat agaccatctg cttcacaagg ccaatgtatt agaaataaat caagataatc
     5521 agtttgtaaa ttttcaatcg atttgttgaa atattcgaat gttttctcat aaccttgata
     5581 gtcattccat aacttcgttg ttataaacaa atcttctcta tcgacgccat tatcctttaa
     5641 tgctcgtcct agtgaagcct cattatcata aaagtatgct gtatcaaacg ctctatagcc
     5701 tgcgtcaatt gcagcattta caactttagt catatcttcg tcagagattt tataaacacc
     5761 taacccaact gaaggcatcg ggtatccatt atttaatatt tgtatctcat tcaacatctt
     5821 tatatctctc caatctatgt atcttttata tctttacatt accctaaatt tttcaacaaa
     5881 ctcaattaat gcgaattatc gctttcaata aaaaatattc attaaatcat taaagatatt
     5941 gagttccaat actattttca cttttcatta attttagtca aaaaaataac caaccaaaaa
     6001 tgaattaaat cattcttagt tggtttatat attaatatct attctgattt ttcatcttca
     6061 tcagactgtc cgatagtagg tctcgcttca ttaaattcat agtttaatat cacccaaata
     6121 atttggtggt tatcgatttc tgaaacaacc cagcgatcat aagttgtatc cacgtaatca
     6181 tctttttgta aattggtatt acgagattgt aaccatccac ctatcgtatc aatatcctca
     6241 gagtcatcaa attctatacc gaactcttca gttaaatcat ccaatagtac tctgccattt
     6301 acttggaatg tcttattatc aattttaacg atatcattca cttcatcatc atcaaattca
     6361 tcacgaattt ctccaacgat ttcttctaaa atatcttcca tcgttaaaat acctgccgtt
     6421 ccaccatatt catctataat aagactcata tgtacatgtt cacgttgcat tctaattaat
     6481 gcatcactga tacgtgttgt ctctgaaatc attggcaact catgtatata gtttgctatt
     6541 ttaatcgttt ttccagaagc gtattcagtt aaaaattctt tgacgttaat aaatccttta
     6601 atgtggtctt tatcaccatc atcagtaatt ggataacgcg taaattgatg ttcttttatt
     6661 gtttctagta attcgtctac attaaaaggt tcatttagtg taatcatttg agttctaggt
     6721 accattatat cttttgcatg tctttcatcg aatgaaaaga tattttgcat atatgccaat
     6781 tcagtttggt tgatttctcc accattataa ctattgttaa taataatttt gatttcttct
     6841 tctgacattg catcagtttg ggcatcagga tttacaccaa acattctaat aataacacgt
     6901 gcagaaccat tcatcagcca aatcaatggt ttcataatgt taccgaaata gaacaatggt
     6961 cttgcatata ctaaagcaag cttttcagta tgttgaatcg ctatagattt aggcgctaat
     7021 tcaccaagta ctacatgcaa atacgtaacg attataaatg acactgcaaa cgaaatcgtc
     7081 gtcgttaatg cagttggtaa attgattgct tcaaatatcg ggtgtaatag cttttcaaac
     7141 gttggttcac caagccaacc taaccctaaa gatgttactg ttatacctaa ctgacaagca
     7201 gaaagataat aatctagatt agcgatcatc ttttttacta ttttagcagg tttatttcct
     7261 tcatctgcta gctgttcaat tcttgttgct ctaattttta ctaatgcaaa ttctgaacca
     7321 acaaatacag tggttaatgc aattaataga ataaatataa tcaaactaat tatggtcgaa
     7381 gtttccaatt aattccctat ttctagggat tcacctccat gttatatgtg tcactcatgg
     7441 gtaacacgca ttcaaattta tcactatgac ttaaattaca actacatgtt atcgccttcc
     7501 catttagaca cccccagaaa aaaatgttat tgcttctatt ttatcataat ataaagtgct
     7561 tatgttaaca gattaaatct attgcataca tttaattatg attatttatt agctaaatgt
     7621 gtgaaccttg ccttgtttta ttgtagattt tacattcata tcatcatcta gaataacaag
     7681 atctgcatcc ttatttactt taatactacc ttttctatca tcgataccta atgcaatggc
     7741 ttgatttaaa cttgttactc gccataaatg atctaatgta tcacctgtaa atgatattaa
     7801 gttacgtaac ccatgattca tttttaaaat actaccagca agcgcaccat ttgcaagacg
     7861 tgcttgttgc gattgaacag ttactttttg tccacccaaa tcatattctc cttcaggcat
     7921 accttttgca cgcattgcat cggtaattaa ataaaaacgt tcattacctt tcatacggta
     7981 agcaattgca accgatgccg gatgagaatg tgtgccatca acaatcattt cggtatgtag
     8041 agcatcattc aaccatgctg ctccaaaaac acctggttct ctatgttgga atggcgtcgc
     8101 tgcattatat aaatgcgtga catgtttagc tcctcgctca acagcttcga ctgcttcttc
     8161 gtatgttgcc actgtatgac caattgaaaa aataatgtca tctttatacg tttcaagcgc
     8221 ttcttttgca ccttcaactt caggtgcaaa cgtcataatc tttattaatc tgttagcagt
     8281 ctcttgaaaa tgtttaattt tatcgataaa tgggcgtaca acgtattgcg gatgttgagc
     8341 accaacttta ttttcagata taaatggtcc ttctaaatgt atacctacaa tttccgctgc
     8401 attgtgaaca tcttgctccg cttcatattt agcaatattt gtaagtgctt tatctatttt
     8461 atcagtagat tgcgtcattg tagtggccaa gtatgatgtc gtcccttcag ataacaaatt
     8521 ttcggataga tattttaagc catcgtatga cccatccatt gcatcttgac cataaccacc
     8581 atgaatatgt atatcaataa aacctggtaa tacatgatga cctttagcat caatcacttg
     8641 aattttattt gtcgtatcat tatcaattgc tgctttatca tccccttctc caattgcaac
     8701 aatctgtcca tctttcacat gaatgtaacc attatcgatt ttgccatctt cagtataaac
     8761 tttgccgtta tatataatta attctgacac gtataaaccc ttctttattt ataaattttc
     8821 actttaagac actcatattt tactatatct gcttcgtttg gcataacttt ttgcaaaata
     8881 ttaaaaaagt ctatacattt gtggtgtcta tttcttcatt catttatcta aaaataaaaa
     8941 caccttcata tcaattcata aaaactgaat taatagaaag tgtttttaca gctttgtata
     9001 agctaccgtt aacacgatgt ataaaactaa catatattca ttgaacttac aacttaatat
     9061 gaagctcttt gctaacaatc attttacatc attaaaacta ctcgtccatt gattttgaag
     9121 cttcgatttc tgtttcagtt aactttggtt taataagtcc ataaattaat gctgaaacta
     9181 atgtaccaac tagaagtgca ataagagttt gaagtaagtg tgcaccatca gtaccaacaa
     9241 ttacaataat accaccatgt ggcgcagtaa ttcgagaacc taagcctaaa gcaattgcgc
     9301 cacctatacc tgaaccaatc atcattgaag gaataacacg taatggatcg gcagctgcaa
     9361 atggaatcgc accttctgta ataaaggaca tacccatcac atagttaggg ataattgaac
     9421 cacgttgttc ttttgtaaat ttacgtctga aaattaacat cgctgtcgca attgctaacg
     9481 gtggaatcat accaccaatc attgcagctg taattggtgc tgcattacct tcaatcaacg
     9541 cacctgttgc aaaaacatat gccgctttgt tgaatggacc gcccatatca atcgccatca
     9601 tagcgccaat aactaaacct aataatacaa tattagaacc tgataaattg tttaatccat
     9661 ctaataacaa atgatttaac caagatgctg gtggattaaa gacataaatc atcaataagc
     9721 ctgtagctgt cactgttaat agtggataaa ttaatgttgg ctttaatccc tctaatgctt
     9781 gtggtaactt acgtgtcatg gctttaacac cttgtgttaa gtaacctgct aagaaacctg
     9841 caataatacc accaataaat cctgaaccac ctgaaattgc taacatacca cctacaagac
     9901 ctgaagcgaa accaggttta tccgcaatac tacgtgcaat gaatccagat aaaattggaa
     9961 tgattagcgc gaatgcactt ttactaccaa tgttccaaag ctgctctgca aacgcattgt
    10021 actctgagct ttttggatta aatgaatttg ctccaaataa aaatacaatt gccattaaaa
    10081 taccaccaga tattacaagt ggcaacatgt tagaaacacc gttcattaag tgtttataga
    10141 atgctttacc tgggcttaat ttttcttgac tgtcatttga gttacctttt ctttgaccat
    10201 cacgggcaac aaaaggttta cgacttgtat ctaatgcttt attaattaat tcttctgggc
    10261 gtttaatacc atctgctact ggtacttcta cgacattttt accatcgaag cgatccgtct
    10321 caacatgaac atcagcagca acaatgatac ctgttgcatt ttcaatatct tgttcagtta
    10381 aatggttttt aatgccgctt gaaccgttcg tttctacttt aattttaata cccatcttat
    10441 ccgcttgttt tttcaatgcg tcacgtgcca tgtacgtatg tgcaatacct gttggacaag
    10501 cagttactgc taacacatat ggctcgttag attgtttaga tgattgttct gcagttgcaa
    10561 cttgttgtgc ttcagcttct gcctcttctt cttttgtcac ttcatcatca gcctcatcta
    10621 taatcgctag tacttcttca ggtgatgaag catgtaataa tttctcgcgt acattttcat
    10681 ccattaaaat accagacaac ttagctaaag catctagatg tgtttgggcg ccaccttctg
    10741 gcgctgcaat cataaagaat aaatgagctg gttgcatatc taaactttgg taatctacac
    10801 cttctttaga tttaccgaat gcgatagctg gagacttaac tgcggctact ttcgcatgtg
    10861 gaatagcaat accttcgccg ataccagttg tactttgtga ttctcgattg tgaatcgctt
    10921 ccttaaatga cgcgacatca cttaatttac ctgctttgtc taattgattt actaactcat
    10981 caataacacc atttttgtca tttgccatta aatccattgc tatcgtatct tttgttaata
    11041 actctgttac tctcattatt ttcactcccc atcaagtacg ctaatcgtaa cttgtgattt
    11101 tattttttct atagcgtccc gtgttgctaa gtcctcatca aatgccgtgg cagtaccgca
    11161 tgcgactgct tgttggaatg ctttttcaat cgttaaacct gaagcaattc cagccaccat
    11221 gcctgcaact gtactatcac cagagccaac tgtattaacc actttccctt gtggattaac
    11281 tgctttaata ctgatttctt tatcaatata aatagcacca tcaccgccaa gcgagacaat
    11341 aacagattgc gcacctttat caactaacaa acgaccatat ttaataacat ctgtgtctga
    11401 gttcactgtt gtattaaaca tcacttctaa ttcatcttta ttaggtttaa taaatagtgg
    11461 atgaaatggt aaaacgcttt cagccaattc tttttcagcg tcgactacta atttagcacc
    11521 tgtctgtgct gtaatttgtg caatttgcgc atacgcatcg cttggaatac tacttggtac
    11581 acttccagca acaataacta tatcttcgct tgttgtattt ttaatttgtt gtaacagttg
    11641 ttcaaattgt gttgacgtta tatgaggacc cggtgcattg atttctgttt cttgtcctgt
    11701 ttttaatttc acattaatac gtgtatcttc atcaacttca ataaaattcg attgaattgc
    11761 actgttattt aatgtatcta taatgaattt cccaggaaat ccacctgcaa atcccaaggc
    11821 agttgactca acatctaatg tctttaagac gcgcgagaca ttaatacctt tccccccagc
    11881 gaatttatat gttgctgttg ctctgttcaa accatcaatt ttaaaatcat tcgtaaaaat
    11941 gacatagtca attgaaggat tgaaagtcac tgtataaatc ataaagtccc tcctataaag
    12001 tgatactttt gttggtattc ttttaacgat tcttgattta atgctttttc agatgtgatg
    12061 attgtcgtac tttctagcaa aggtacacga gcaaaatata ctttattaaa cttagaatga
    12121 tctataagta caaatgattg attggctaat gacattgctg tttgtttaac taatgcctct
    12181 tgctcatcgg gagtagttaa tccaagttca atatctaatc cattcatccc gataaaagct
    12241 ttatcgaaac aatatcgtct taatatctcc atagcactag aaccaatcgt agcaagtgta
    12301 ttttctttaa cttgaccacc tagcataatt gttttaatac cttttttaag taaagcttct
    12361 acatgtgtta aaccattggt taccacaatg atatctttcg cttgaatata tttaattagc
    12421 tccaatgtag atgaaccagc atcgataaat aagcattcat tatcgttgat ttgattagct
    12481 gctattttag caatcatttt cttttcatca agattcgttg ctaatttttc agttaaattc
    12541 gcctcaatca tacgattttc ttttaacatt gcaccaccat gcacacgttg caatttccct
    12601 aattgttgta gtttagataa atctcttcgt attgttgaag cactgcaacc agttcgatct
    12661 attaattctt gtaaagtcaa aaaatctttg tgcgaaagtt cttctaatat taactcgtgt
    12721 cttttttcag taattatcat catcacccca tctcactttg tattataatg agtacgcttt
    12781 cacttttcaa tcataaatta ccaaaaacaa tcacaaacac ccaaattgtg acgataaaat
    12841 tacattttta tgttcaaata ctcaattata atgactgttt atgatcgtaa aatgctaatt
    12901 caatcaaaaa ggagtgtggc agaaatgaat tttataaaaa tttcatctct gccacactcc
    12961 taatcttgcc ccatactttt tgttgttatt aatcattcat ggataactgc tccaattttg
    13021 cctttagtta ttgtaatcaa atcctcaaca gctattgtga tttgcattgt tcgaagccca
    13081 ccactcacac tgatatgact ataattttca cacgatttgt caacgactgt tggaaacaat
    13141 gttttcatac caacaggcga acacccacca cgaatgtatc ccgttacatt tttcaaatta
    13201 tctaaaggca taagctgcaa tttcttctct ccaaccaaag cagctgcctt tttcatatct
    13261 aaagtttcac ttactgggat aacaaataca aaatgttcat gttttgtatt ttctagaaca
    13321 agcgttttaa atactgtttt aacatttgct tgtataagtt ctgcgacttc tgcacccgat
    13381 aaatgttctt ctggtacctc aaagctatga acttcgtatt taatttttgc acgatcaagc
    13441 atacgcatcg cgttcgtttt cttattttta gccatttcaa cacctcattt atacttcttc
    13501 agaactatat taacatttta aaatacatta cggattggtt gattttgaca taacatttac
    13561 atcattttat aagttgattt caccttttta aattaaatta ttgcaaaata aaaacagtat
    13621 atccaattag aatacactgt tccatctatt atttcgttat tatgccttga tttgaaatac
    13681 ttacatgttg cttactagtt gaattatcat tggaataaac ataattatat ccatgatttg
    13741 tcttagaaat tctttctaaa ccataattat cattgatatg cttatctgca taacgatcag
    13801 caataacttg gcactcttgt tcgttaaaag aatttgattt atttgcattc acacgatgtt
    13861 taaaatgatt ttgcttttcc acattttcat tcacttcaga tgctttagca gtatgaatat
    13921 tgagaatatc cattttcttt ttataagtat cttcagttga tacatttatc gcgttaagcg
    13981 tattaattgt tttatgtacc ttagcaagcg catcttcatt tgttgctaat attaataaac
    14041 caattaaaca aacggttaat attacagcaa caaagcctaa aaataaattt tttactgtca
    14101 tccccttacc cacatttcct tactcatttt ttatcatctt aacacattaa cgttaatgaa
    14161 ttaggtatgt ggattgcaat ttaaaaagaa tgtaatattt gtaacatgtt tattactcaa
    14221 aatcattttt tgttacatat aacatttaaa tataaagagt aaaacattta gtcaatcgaa
    14281 tctttttaaa tgtaaagcat cgaattttct taataaaatt cataagaaaa acgatgctaa
    14341 tcattcataa tattacgctt tactttatat caatatacac caaaatactt atgctacata
    14401 ttttgttctt tcaattttgc tctatgttgc ttttcaatta aaacaataac aacacctgct
    14461 aatgaaacac ctatagccat ataaattggt gcttcaatgt gtacatcaaa taacgcacct
    14521 gcgattaaag gacctatgaa attacccata ctagtgaatg tcgagttcaa tccgcctgca
    14581 aagccttgcc tttctccagc aatattagaa aaataatttg taatggctgg tcgtatcata
    14641 tcaaaaccta tgaagacaac aaaactgatt aacattattg accaatagcc attagcaaaa
    14701 actaataata ttaagacaac aactgaatat aataatgacc aagctataaa tgttaactct
    14761 gagaaatact tcataaattt atcgaagaaa tagatttgga aaagtgcccc aaatataccg
    14821 ccacccgtaa tagcaatcga aatatcttta ggtgaataat ttaccttgtc agctgtgtat
    14881 agtgaataca atgtttcaaa tgcagataaa ccaaacgata atacaagtgt taaaataact
    14941 ggtgtaataa acactttcca gttaattttc gttagcaatt gtggctctaa cttttggaaa
    15001 ccacttgtcg tagacttttt cggatcgtga atcaatacaa ttgacattat aaatgctaga
    15061 atacctaatg ctcctgcaaa gtaaaatggc atacgatgtg aaacttctgc cataaatcca
    15121 ccaatccctg gtcctaaaat gaatccagaa ttgataatcg ctgacatgta gccaaagttt
    15181 tttgcttttt gatggcttgg tgaaatgtca gctattaaac ctgtcacacc aggcattacc
    15241 ataccagcac tcataccacc aatcactctc gataacatca ataccgaaaa attgtggcca
    15301 actgcaaaca taaattctga cactgaaaac aaaattaatc ctatacatat aattaatttc
    15361 ttccctaatt tgtcagctag cgtaccacca aacggcgata taatcatttg agataacgca
    15421 aaagcagcaa ctagtaatcc taaatcacta ccagttaatc ccaaatcttt taaataaaca
    15481 ggcaagactg gtattactaa accgatacct aaaaaaatca agaaaatatt aaaatataag
    15541 acaaaaatct gtttattcat atgctcacct cttttttctc catgtcatgc ttaaagctgt
    15601 tattcttctt ttccacgaca gattgcaatt catgctaaat attcgtaaaa ggtttctata
    15661 ttgtattaca acattgctac atttgacaat attttttctt tgtaattatc actattttcc
    15721 atttaattgt ataattaatg acatattaaa ataaagggga ttgatcatat gaaattgcaa
    15781 aaaagtaatc atactatact actagtgttg gaaaaaggag aagatatcgt tgaatgtata
    15841 acaacctttg cagatgacca agacttaaca tttacatctg tcagcggcat tggtgcatgt
    15901 gatgacgttg tattaaaatt tttcaattta acaacgaaac aatacgaaga aaaacatatt
    15961 actgagccac tggaattgac gagcttatta ggtaatattt ctcgtctaga caatggtcat
    16021 tttgcacatt tgcatgctac tttcggtact caatcatatg aaacttttag cggacatctc
    16081 gctaaagcaa ttgtttctgc aactgctgaa atcattctta ctgtaactga tttggacatt
    16141 caacgttcat tcaaagatgc agtcggttta aatttacttg atcctcaata gcactttact
    16201 ttttagttga atcaaatttt attacaagtt atcataagtg cttaatcata aatgcattct
    16261 cgtttattaa tatacttcaa cgagttaaat taaattttta attaggaatt gaatgcgttt
    16321 ataactatca catttgaatg aataactttt tcattagaaa gtagtgttta ttattgatgc
    16381 aatctcgata catcgctaga atttactttt taattttatt tatcgtctcc ctttttgaga
    16441 ctaaaatttt tcaatttatg actttaaatt tgtttttagc atatatacct tttgagctta
    16501 gtctattatt acgattattc aaaccgaata aaaagtatga atggccactc tttatcatat
    16561 atagttttat atttgtgtta cttttaccaa atacatttta tatggtaact gatttaatcc
    16621 atttgaacca atttcaattc aacttttacg caggactaaa tttaagtgag tggaaatact
    16681 ttacctattt actgcttggt gtcttcttag cggtttatgt gatgatttta atctattttg
    16741 aaatactaac attgacttct caccgttggc ttaatagatt aattgttgtc gtgctaatgt
    16801 ttttaaatgg attcggaatt tatattggtc gtttcttacg cttacattcc gtttatttct
    16861 ttaatgagcc cttacatgtt ctaaatcaca ttctagaaag tctaacgctt aaaactgcta
    16921 tgtttgtatg ttttatggtt gtcatgcaag cagcaattct attatttgga aaaggagtgc
    16981 gtttgaacaa gtgacaataa atataatcgt tttaatagta tctatcattg tctttcaatt
    17041 gattattgga cacatatggc atgatattgg tttaagttat ttaagaagta tcttactcat
    17101 gatgctgcca tttggactag gtgtatttat acaacaagtg tcgtattacg aacgacaata
    17161 tcctaaatgg caagtgcctc aaaacataaa agttagattg aaatatattt atctcgctac
    17221 gtttttagag tatgtcgtac tatacctcac tttatttacg gacatactaa gataacatca
    17281 atttaataag aaaactaaat acaacttata tatttgagga aagccaattt ctatcacatc
    17341 aataatagaa attggcttat ttatattgct taagaacttt atattctgct ccagatttta
    17401 ctcatgccag atctatcaat tgagccgaat tagtctagcg ctttaaatgt agctaaaact
    17461 tgtgtacgtt tttcttgatg atctaccatt tgtcttggat aatgactacc taattcaatc
    17521 ccctgttcaa aaaggttgga ctcattgcgt tgtgtatcat gcaaatattt tgcatcaatt
    17581 tgattaaaaa tcggaagata tgttttgata tacaaagctt tagcatcaaa gcgttcactc
    17641 tgtcttattg gattaaacat tctaaaatac ggcactgcat ccgtacctgt agaagcagac
    17701 cattgccatc catgaatatt tgatgctgca tcatagtcaa taaggtgctt tctaaagaat
    17761 ttttctcccc atgtccaatc tataaataaa tctttggtta aaaattgcga cacaaccatt
    17821 ctcattcgat tatgcataaa accagtttgt gtcaattcca ttattgctgc atcaatgatt
    17881 ggaaatcctg tttgcccttc gcaccatgca ttaaaattcg ctgcattttg cgaccatttt
    17941 atctgtcgat atttaggttt gaaagcttgg tatgaggttt ctggatactg tgtcattaac
    18001 acataataaa attctctaaa aatgagttca cgtataaatg cttcatggtt tgcctcatct
    18061 tcatcataac gcgccaataa atcattaaaa atttcaataa tatctaataa tccatatgct
    18121 aaagcaacac ttagctgact tgttaacact tcaggtaaat agtctcttcc gctttcgtaa
    18181 ttctgtatat cttgatctaa aaaatgttgc catttattct gttcaattac ttgtgaggat
    18241 ccaaacgcta tgtcatcaaa cactaaatca tcaggtgatg caatgacaaa gtcttcgaat
    18301 gattttaaat catagtgata tacgtctcta tgcctcaagt aaggtcgcca ttttctatag
    18361 aaactagtaa aaactttgta tggctcccct tgttgattca ttgttttact cgctttaaag
    18421 taatgattcc cacgaattaa agtaacggca atacgtgctt cattgaataa agaacgttga
    18481 tgtaaaatat catattcttc aagatgataa ctcataatat cacccgcaat cactacttca
    18541 gagatattgt tctctttaca aaatgaaatg atatcgtcat aagacataac ataaggtgta
    18601 agcttttctg cgtgcaacga ttggtaaaag cgctgcaaag taccgcgata aaactgtgct
    18661 ttcaaatcag aagctgaaga catgtcctct tccggaatga taaaataaca tgcatcaatg
    18721 tcttcttttt gttgataaat atattcaaat aatggattgt gttccattcg aaacattcga
    18781 tttaataaca cagcaattgc cataaacaaa acacccttca attatctttt tattttatta
    18841 agctttttgt cactttaaaa taacctagat tcttatttat atttttaatt aaagcaaacc
    18901 taaccatttc caataagtca tactaaaaac tatcactaga atatacccta caattgtcaa
    18961 tggtatacct gccttcaaga aatctttaac cgtaaaagta ccagtgccat aagccaacat
    19021 attttgaggt gcactaacag gtaataagaa accaaaacta ataacaaatt gttgaattaa
    19081 aacaaatcct atagactggt ctcctaagtg taacgtagag gttagcgaaa taaaaacagg
    19141 tattaacgct gatgataaac ttgttgcact cgcaaagccc aaatgaatca atatattaaa
    19201 aagcgtgata agtgcaattg tcgcgataat aggtaaatgt tttaaaccta aaacaccaaa
    19261 agtttgatca cttaaccatt gagctgcacc tgttttcaaa agaacgttcc ctagtgaaat
    19321 acctacacca aacacgataa ttgttcccca tggtatttta ttttcaacat gtttccatgt
    19381 catgacacca attttcggca ttaacataac acctaaagca ataatagtaa tggatgcaga
    19441 gtcaatcgga tgtaatactt tttcagttga ccaaaacagt aataataaca tcgatataac
    19501 aattaaacgc cattcacgtg ggctaacggg gccaagttta tgcaattctt cttttattaa
    19561 atctttacca ccttctattg tattaatttc tggaggcatc actttaatca tgatgaaata
    19621 taaagctacg gacataacta tggaccaagg cgctgcatat aagaaccact cgccccatga
    19681 aacatcaaat cctaattgat ggtttataaa attaatcgct acgatatttt gtgctgccgc
    19741 cgttttgata ccaatattcc aaattgacac agcttgtact gaagtaatta ttaataaaga
    19801 cgctaacttg ctatctttgg aaactttaaa tgccgcaatc atacccagca agattggtac
    19861 aactgcccct gctctagctg ttgcagaagg aacgaaaaat gcaagtacaa ttgaaacgat
    19921 aattgctcca ataactatat ttctagtttt attaccaaca attgataaca ctaaaagagc
    19981 tagtctttta tgcaaattcg tttcttgcat agcagcagcc aaaaataatg cagctgcaac
    20041 gagagctaca gctgaagttg catatccact aaacgctaat gataatgcat gattagttcc
    20101 tagaaggtca cttccagcta aaatagcact gccacttttc ggattaccta gcttctcccc
    20161 taaattttga accgggctaa atcctaaaag taatatcatt aagccaataa ttaaagttgc
    20221 tgacaccgga tatgatacag cttccgttac ccacataata actgcaaaag ctaaaatagc
    20281 tagtactgcc ttagccatta tcggcagact tgaaggtgcc gggagtaata gtacaactat
    20341 aagtattata aaactcatga caatccaaaa tggcttgtat tgcttttttg tcttttcttt
    20401 cttttgttgt tcatgttcca ctacatgttt ttcttctgac ataacagtca cccctttgtt
    20461 taatcttaat ttaattataa caatttttta aaacatacat ttttaattta tgcaaaatac
    20521 acaattttat cgaaatattt tgtaaataat attgctaggt gatgagttat ctcggttaat
    20581 tacaaaacga tatctaagtt taaatattag atcataaaaa aactatctac tgaatatcct
    20641 catagcatca tttttctaag caaaagttaa acttagttag ataaaatgca aatgagatta
    20701 ttgtagatag tctcttttta atatttaaaa atgattaatg ccataaacat aaataattcc
    20761 aaagaatgcc gcgccactaa gcactgttaa tataagactt agtaacaatg tgcgtttata
    20821 atgtttaata aatgctatga acaaaatgac tacattatac gcaaagaaaa gtccgaataa
    20881 tgtcattgtt attttcaaat cagaatagaa aatatttaat agtaatacaa tgactgcata
    20941 aattaaatac ggaataacta taaatttgcg ttcaaatctt aattgttcga aacgtttatc
    21001 ttcgtttgtc atgatatgct cctttatgat ttaaaagtaa ataagtttac ggaatatctt
    21061 gtccgataat agcggtgtaa atgtcaaacc acgactgatc atctaaatta agttgtagcc
    21121 cttcgattgc ttgatcaata cgctttaact gacttgttcc aagtatcggc atgatactat
    21181 gcggtatttt tactaaccat gctatcatca cagctgtgtc actcacacca tatttgtcag
    21241 ctattgattg aacaactttc ataatacgtt gcgctttaat atcttccttg tcgaaaattt
    21301 taccgcctgc aaaaggactc caagccataa tttgaacatg gttttgatac attgaatcca
    21361 ttgttccatc ttgtaaacta tcaacgtgat atggcgataa ttctaattga ttgatgctaa
    21421 tatgtagtct ttctttcata atatattgat ttaacaattg gtattgtgaa tgattaaaat
    21481 tcgacacccc gaatgacttc aacttacctt gtttaacaag tttagttaat gcatcagcaa
    21541 cttgttctgg atccatcaat ggtgaaggac gatgaatgag tagactatct aaataatcta
    21601 cattcaaatt gattaatgac tgttcaacag atttcacgat gtgcttacta ctcaaatcat
    21661 aacgatgtcc atttgtaaaa tcaaattgct tagaaggcaa aatgatacca catttcgtaa
    21721 caatttgaat tttatttctt aattcgggtg ataaatccaa agcattacca aacagtgatt
    21781 cacattgata atccccataa atatcagcat gatccatcgt tgtaattcca cgttcaacta
    21841 attcatttat aaaataattt aactctttcg cagtcatctt ccattcattt gcacgccaaa
    21901 aaccttgtac aagcctagaa aaatgaacat agtgattaat cattatttgt tccatatctc
    21961 atccactcca tccctcaatg tcacaaaata ttcacaataa aagtattaag aaattaatgt
    22021 gctatttccg ttaaattgaa acaacacatt ttctgattta cgtaaattgt agcatatcca
    22081 attgattacg tatgtgattt gtatctaaag tttcaccaat aataacaatc gttaatggta
    22141 attgcatgcc aattattcca tagtctggta aaccatatgc atattgaaat tcataaattg
    22201 catttggttg atttctaaat gacacataac ctttcaaacg tagaacagat tccggtaatt
    22261 tcattataaa ttgataaaac aattgacgat caatcggacc tgtaaacgtg taagtcatac
    22321 ttttaatccc atgatgatgg tgatgatgag acgatatctc tctgtcttta acagttaagt
    22381 ccaattcttc caacgaaact tttccatagg ttgttttata agttggaata cttgcacaaa
    22441 tcatacctaa ttgcttatca attttctcaa gactgtcatc agttataaga tcaattttat
    22501 taataataat catgtcactt agtttcaact gttcttccat cagcgaaact gtattttcgg
    22561 tatattgatg acgttctaaa aatcgagtcg catctaatac accataaata atcggctttt
    22621 caaagaaatt aacgatttgc ggatcttgac atgcaactag taattccaaa ggatgcgcaa
    22681 tgcctgtcgc ttctatgatg acatgattaa cgtccccttt taaagcaatg gcttttagtt
    22741 catagacaag ttcttgttta agatcgcaac aaacacaacc attaatcaat gaatggactt
    22801 caatttcatt tgaaatacta ttgctatcaa tatcaaaagt accgaattca ttcatgatga
    22861 ttttaatttt ctcatcattt tttaataatt cactaatata atgtgtcagt aacgtggttt
    22921 taccactacc caaaaatccg ttaat
//
LOCUS       DANBWH010000017        22459 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.017, whole genome shotgun sequence.
ACCESSION   DANBWH010000017 DANBWH010000000
VERSION     DANBWH010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 22459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22459
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.017"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(130..396)
                     /locus_tag="PPY92_000457"
     CDS             complement(130..396)
                     /locus_tag="PPY92_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDJ2624481.1"
                     /translation="MSNYTVKIKNSAKSDLRKIKHSYLKKSFLEIVETLKNDPYKITQ
                     SFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLILSAWSHYD"
     gene            complement(396..653)
                     /locus_tag="PPY92_000458"
     CDS             complement(396..653)
                     /locus_tag="PPY92_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDJ2624482.1"
                     /translation="MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLE
                     DWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDIDWDNL"
     gene            1012..1467
                     /locus_tag="PPY92_000459"
     CDS             1012..1467
                     /locus_tag="PPY92_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1307 domain-containing protein"
                     /protein_id="HDJ2624483.1"
                     /translation="MKKLCSLIVVALICIIALSACGKEQTKTYEGDVSGKHVLTSITY
                     KDDKVLKQSTINTIKYDDLGMDKDEAKKLFAKSESIFKDLKGVKYKVDYKDKKAIEHL
                     DIDYTEVDMKKLNKRLGVSTKENKDISFEKLEKQLKHRGLKEKDKMDDK"
     gene            complement(1635..3212)
                     /locus_tag="PPY92_000460"
     CDS             complement(1635..3212)
                     /locus_tag="PPY92_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HDJ2624484.1"
                     /translation="MTFLTVMQFIVNIIVVGFLLTVIVIGLIWLIKDKRQSQHSVLRN
                     YPLLARIRYISEKMGPELRQYLFSGDNEGKPFSRNDYKNIVLAGKYNSRMTSFGTTKD
                     YQDGFYIQNTMFPMQRNEISVDNTTLLSTFIYKIANERLFSREEYRVPTKIDPYYLSD
                     DHAIKLGEHLKHPFILKRIVGQSGMSYGALGKNAITALSKGLAKAGTWMNTGEGGLSE
                     YHLKGNGDIIFQIGPGLFGVRDKEGNFSEGLFKEVAQLSNVRAFELKLAQGAKTRGGH
                     MEAEKVNEEIAKIRNVEPYKTINSPNRYEFIHNAEDLIRFVDQLQQLGQKPVGFKIVV
                     SKVSEIETLVRTMVELDKYPSFITIDGGEGGTGATFQELQDGVGLPLFTALPIVSGML
                     EKYGIRDKVKLAASGKLVTPDKIAIALGLGADFVNIARGMMISVGCIMSQQCHMNTCP
                     VGVATTDAKKEKALIVGEKQYRVTNYVTSLHEGLFNIAAAVGVSSPTEITADHIVYRK
                     VDGELQTIHDYKLKLIS"
     gene            3407..4156
                     /locus_tag="PPY92_000461"
     CDS             3407..4156
                     /locus_tag="PPY92_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDJ2624485.1"
                     /translation="MSNTNKHYIEEEYATEQSRFFKRDIGFIFLYIFLVNILPIIIVF
                     LTFGLVAVFTQQAPWGFSFPISMNISYLLAVIISLAIFRRMHEDAFVPMIRTQIIAST
                     KYKWHILISFTLSVVLFLLLNYFDIFALNVDAFTVFDIYPLIRDYDSISLIVMLWLTI
                     ILNTLICQLIFRHILIQELSRLIPLKITIVLSVILETLYYYPYINTWWEFIPALILAS
                     SATYLYLKSNRNFMVSYFYQLAVILVLHIFM"
     gene            complement(4518..5711)
                     /locus_tag="PPY92_000462"
     CDS             complement(4518..5711)
                     /locus_tag="PPY92_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDJ2624486.1"
                     /translation="MKGAMAWPFLRLYILTLMFFSANAILNVFIPLRGHDLGATNTVI
                     GIVMGAYMLTAMVFRPWAGQIIARVGPIKVLRIILIINAIALIIYGFTGLEGYFVARV
                     MQGVCTAFFSMSLQLGIIDALPEEHRSEGVSLYSLFSTIPNLIGPLVAVGIWNANNIS
                     LFAIVIIFIALTTTFFGYRVTFAEQEPDTSDKIEKMPFNAVTVFAQFFKNKELLNSGI
                     IMIVASIVFGAVSTFVPLYTVSLGFANAGIFLTIQAIAVVAARFYLRKYIPSDGMWHP
                     KYMVSVLSLLVIASFVVAFGPQVGAIIFYGSAILIGMTQAMVYPTLTSYLSFVLPKVG
                     RNMLLGLFIACADLGISLGGALMGPISDLVGFKWMYLICGMLVIVIMIMSFLKKPTPR
                     PASSL"
     gene            complement(5723..6472)
                     /locus_tag="PPY92_000463"
     CDS             complement(5723..6472)
                     /locus_tag="PPY92_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDJ2624487.1"
                     /translation="MIKVTDVEKSYQSAHVFKRRRTPIVKGVSFECPIGATIAIIGES
                     GSGKSTLSRMILGIEKPDKGCVTLNDLPMHKKKVRRHQIGAVFQDYTSSLHPFQTVRE
                     ILFEVMCQCDGQPKEVMEVQAITLLEEVGLSKAYMDKYPNMLSGGEAQRVAIARAICI
                     NPKYILFDEAISSLDMSIQTQILDLLIHLRETRQLSYIFITHDIQAATYLCDQLIIFK
                     NGKIEEQIPTSALHKSDNAYTRELIEKQLSF"
     gene            complement(6465..7280)
                     /locus_tag="PPY92_000464"
     CDS             complement(6465..7280)
                     /locus_tag="PPY92_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDJ2624488.1"
                     /translation="MTLLTVKHLTITDTWTDQPLVSDVNFTLTKGETLGVIGESGSGK
                     SITCKSIIGLNPERLGVTGEIIFDGTSMLSLSESQRKKYRGKDIAMVMQQGSRAFDPS
                     TTVGKQMFETMKVHTSMSTQEIEKTLIEYMDYLSLKDPKRILKSYPYMLSGGMLQRLM
                     IALALALKPKLIIADEPTTALDTITQYDVLEAFIDIKKHFDCAMIFISHDLTVINKIA
                     DRVVVMKNGQLIEHGTRESVLHHPEHVYTKYLLSTKKKINDHFKHVMRGDVHD"
     gene            complement(7277..8146)
                     /locus_tag="PPY92_000465"
     CDS             complement(7277..8146)
                     /locus_tag="PPY92_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDJ2624489.1"
                     /translation="MIILKRLLQDKGAVIALGIIVLYVFLGLAAPLVTFYDPNHIDTA
                     NKFAGISFQHLLGTDHLGRDILTRLIYAIRPSLLYVFVALFVSVLIGSILGFLSGYFQ
                     GFVDALIMRACDVMLAFPSYVVTLALIALFGMGAENIIMAFILTRWAWFCRVIRTSVM
                     QYTASDHVRFAKTIGMNDMKIIHKHIMPLTLADIAIISSSSMCSMILQISGFSFLGLG
                     VKAPTAEWGMMLNEARKVMFTHPEMMFAPGIAIVIIVMAFNFLSDALQIAIDPRISSK
                     DKLRSVKKGVVQS"
     gene            complement(8143..9078)
                     /locus_tag="PPY92_000466"
     CDS             complement(8143..9078)
                     /locus_tag="PPY92_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDJ2624490.1"
                     /translation="MFKFILKRIALMFPLVIVVSFMTFLLTYITNENPAVTILHAQGT
                     PNVTPELIAETNEKYGFNDPLLIQYKNWLLEAMQFNFGTSYITGDPVAERIGPAFMNT
                     LKLTIISSVMVMITSIILGVVSALKRGKFTDRAIRSVAFFLTALPSYWIASILIIYVS
                     VKLNILPTSGLTGPESYILPVIVITIAYAGIYFRNVRRSMVEQLNEDYVLYLRASGVK
                     SITLMLHVLRNAIQVAVSIFCMSIPMIMGGLVVIEYIFAWPGLGQLSLKAILEHDFPV
                     IQAYVLIVAVLFIVFNTLADIINALLNPRLREGAR"
     gene            complement(9091..10689)
                     /gene="cntA"
                     /locus_tag="PPY92_000467"
     CDS             complement(9091..10689)
                     /gene="cntA"
                     /locus_tag="PPY92_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine-dependent metal ABC transporter
                     substrate-binding protein CntA"
                     /protein_id="HDJ2624491.1"
                     /translation="MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDI
                     GDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLAKKWDVSEDGKTYTFHLRDDVKFH
                     DGTTFDADAVKKNIDAVQQNKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAE
                     LAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSK
                     LNKVQAKVMPAGETAFLSMKKGETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQP
                     MNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQEKPATQLFAKNVTDI
                     NFDMPTRKYDLKKAESLLDEAGWKKGKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEY
                     LQAEFKKMGIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKAKNGY
                     ESATSGIENKDKIYNSIDDAFKIQNGKERSDAYKNILKQIDDEGIFIPISHGSMTVVA
                     PKDLEKVSFTQSQYELPFNEMQYK"
     gene            complement(10832..12133)
                     /gene="cntM"
                     /locus_tag="PPY92_000468"
     CDS             complement(10832..12133)
                     /gene="cntM"
                     /locus_tag="PPY92_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_096659686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine dehydrogenase CntM"
                     /protein_id="HDJ2624492.1"
                     /translation="MSKLLMIGTGPVAIQLANICYLKSDYEIDMVGRASTSEKSKRLY
                     QAYKKEKQFEVKIQNEAHQHLEGKFEINRLYKDVKNVKGEYETVVMACTADAYYDTLQ
                     QLSLETLQSVKHVILISPTFGSQMIVEQFMSKFSQDIEVISFSTYLGDTRIVDKEAPN
                     HVLTTGVKKKLYMGSTHSNSTMCQRISALAEQLKIQLEVVESPLHAETRNSSLYVHPP
                     LFMNDFSLKAIFEGTDVPVYVYKLFPEGPITMTLIREMRLMWKEMMAILQAFRVPSVN
                     LLQFMVKENYPVRPETLDEGDIEHFEILPDILQEYLLYVRYTAILIDPFSQPDENGHY
                     FDFSAVPFKQVYKNEQDVVQIPRMPSEDYYRTAMIQHIGKMLGIKTPMIDQFLTRYEA
                     SCQAYKDMHQDQQLSSQFNTNLFEGDKALVTKFLEINRTLS"
     gene            complement(12126..12944)
                     /gene="cntL"
                     /locus_tag="PPY92_000469"
     CDS             complement(12126..12944)
                     /gene="cntL"
                     /locus_tag="PPY92_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-histidine (S)-2-aminobutanoyltransferase CntL"
                     /protein_id="HDJ2624493.1"
                     /translation="MNNFNNEIKLILQQYLEKFEAHYERVLQDDQYIEALETLMDDYS
                     EFILNPIYEQQFNAWRDVEEKAQLIKSLQYITAQCVKQVEVIRARRLLDGQASTTGYF
                     DNIEHCIDEEFGQCSITSNDKLLLVGSGAYPMTLIQVAKETGASVIGIDIDPQAVDLG
                     RRIVNVLAPNEDITITDQKVSELKDIKDVTHIIFSSTIPLKYSILEELYDLTNENVVV
                     AMRFGDGIKAIFNYPSQETAEDKWQCVNKHMRPQQIFDIALYKKAAIKVGITDV"
     gene            complement(12955..13776)
                     /gene="cntK"
                     /locus_tag="PPY92_000470"
     CDS             complement(12955..13776)
                     /gene="cntK"
                     /locus_tag="PPY92_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine racemase CntK"
                     /protein_id="HDJ2624494.1"
                     /translation="MNRQVIEFSKYNPSGNMTILVHSKHDASEYASIANQLMAATHVC
                     CEQVGFIESTQNDDGNDFHLVMSGNEFCGNATMSYIHHLQESHLLKDQQFKVKVSGCS
                     DLVQCAIHDCQYYEVQMPQAHRVVPTTINMGNHSWKALEIIYETYVHYVIPVKQVTTE
                     IQHLVEAFVREQQWSHKYKTVGMMLFDEQRQFLQPLIYIPEIQSLIWENSCGSGTASI
                     GVFNNYQRNDACKDFTVHQPGGSILVTSKRCHQLGYQTSIKGQVTTVATGKAYIE"
     gene            complement(14466..15233)
                     /locus_tag="PPY92_000471"
     CDS             complement(14466..15233)
                     /locus_tag="PPY92_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDJ2624495.1"
                     /translation="MNIRHIRNATAVIEYGGKRILIDPMLSDKGSFDPFPNSPRQDQK
                     NPLVELPMAIEDIINDIDLVILTHLHIDHFDPKAIEVLPKDIKIYAQNEEDASEVERY
                     GFTNVSVFNDVTHIGEIELIKTDAQHGHGEILKMTGHVHGMILKHSEEPTLYLAADTV
                     WFEGVEKALKTYQPDVVVLNGGANQFFEGGPLVMDEHDVLKVANTLPLAQIVVVHMEA
                     VNHWHLSRKELNEFINSNDLGNRVVVPNDGELLTFEK"
     gene            complement(15333..16151)
                     /locus_tag="PPY92_000472"
     CDS             complement(15333..16151)
                     /locus_tag="PPY92_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDJ2624496.1"
                     /translation="MKRLENKVAVVTGASTGIGQASAIALAQEGAYVLAVDIAEAVSE
                     TVDKIKSNGDNAKAYNVDISDEQQVVDFVSDIKEQFGRIDVLFNNAGVDNAAGRIHEY
                     PIDVYDKIMNVDMRGTILMTKMMLPLMMNQGGSIVNTSSFSGQAEDLYRSGYNAAKGA
                     VINFTKSIAIEYGRDGIRSNAIAPGTIETPLVDKLTGTSEDDAGKTFRENQKWMTPLG
                     RLGKPEEVAKLVVFLASDDSSFITGETIRIDGGVMAYTWPGEMLSDDSWKRTLE"
     gene            16386..17924
                     /locus_tag="PPY92_000473"
     CDS             16386..17924
                     /locus_tag="PPY92_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDJ2624497.1"
                     /translation="MTSKHQQKGSIVNRFLNSVEKIGNKLPDPSVLFFLMCVGLAIMT
                     WVISLFNVSVKHPGTHQTIYIKNIISHDGFTMIMNDTIKNFSEFPALGLVLAVMIGIG
                     VAEKTGYFDKLMISVVNRAPRFLILPTIILIGILGSTAGDAATIILPPLAAMLFIKIG
                     YHPIAGLTMAYASAVGGFAANIVVGMQDALVYSFTEPATRIVSDSIKTNVAMNWYFIA
                     ASVVVLLPTILLVTTKLIIPRLGKYDDSLMHDDHEEASSHITDKEAHALKWANISFIV
                     TIILLIITAIPEHSFLRNAKTGSLLDDAPLINGVGLIILVVFLVPGLVYGILSKEIKN
                     TKDLGKMFGDAVGSMGTFIVIVFFAAQLLAYLKWSNLGIIAAVKGAKLLEHQNGIVLI
                     LGIIVLSAMVNMLIGSASAKWGILGPIFVPMLILIGFHPAFTQVIYRVGDSITNPITP
                     MMPYLPLLLTYAQKYDKRMKLGALLSSLMPYSIALSIVWTVFVIIWFLLGIPVGPGGP
                     IFVK"
     gene            complement(18037..18459)
                     /locus_tag="PPY92_000474"
     CDS             complement(18037..18459)
                     /locus_tag="PPY92_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDJ2624498.1"
                     /translation="MPYNYKKQNGELMSVMSQGEKFIHQSPVNDELSALIKLLISKIN
                     GCHYCVDIHKKELKELGVTQMKIDEVLSFRHLDLFTDQEKVTLEFAEMLNSIKDFKKF
                     EIIDRLKSFYDEEQIIDLVFVVNQINGWNRLNIISDRL"
     gene            complement(18911..19330)
                     /locus_tag="PPY92_000475"
     CDS             complement(18911..19330)
                     /locus_tag="PPY92_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDJ2624499.1"
                     /translation="MNKKHVFVIIGVILCICIVASVIYLKVKYDEKEKQKAIYYKEQQ
                     ERITLYLQHNTKEPNTIKSVHFTSLKRGPMGDAVIEGYINENKKDNFTAYATPEHNYQ
                     FGGAMIESERLSELLKPAHQLKSPDEIKEELDKKEGH"
     gene            complement(19357..19767)
                     /locus_tag="PPY92_000476"
     CDS             complement(19357..19767)
                     /locus_tag="PPY92_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDJ2624500.1"
                     /translation="MSKKKILILTSIMLIILISVASIYFKMKYDEKEKQKSIYYKEQQ
                     ARITLYLKHNTIEPNTIKSVHFTKLETSPMGSAVIEGYINENKEDDFVAYASPENNFQ
                     FVGDLIKSERLSELLKPAHQLKSPDDIKKELNKK"
     gene            complement(20034..20456)
                     /locus_tag="PPY92_000477"
     CDS             complement(20034..20456)
                     /locus_tag="PPY92_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDJ2624501.1"
                     /translation="MNKKHVFIIIGVILCICIVASVIYLKVKYDEKEKQKAIYYKEQQ
                     ERITLYLNHNTKEPNTIKTVHFTSLKRGPMGDAVIEGYINENKEDDFVAYGSPEHNYQ
                     FGGSLIKSKNLSTLLKPVHQTKSPDEIKKELESKKNDR"
     gene            complement(20706..21401)
                     /locus_tag="PPY92_000478"
     CDS             complement(20706..21401)
                     /locus_tag="PPY92_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDJ2624502.1"
                     /translation="MTVLTDKIAVVTGAGSGIGEAIATLLHEEGAKVVLAGRNKDKLQ
                     NVANQLAQDSVKVVPTDVTNKEEVDELMKIAQQTFGGLDIVINSAGQMLSSKITDYQV
                     DEWDSMIDVNIKGTLYTAQAALPTMLEQSSGHLINIASISGFEVTKSSTIYSATKAAV
                     HTITQGLEKELAKTGVKVTSISPGMVDTAITAAYNPSDRKKLDPQDIAEAVLYALTQP
                     KHVNVNEITVRPV"
     gene            21690..21971
                     /locus_tag="PPY92_000479"
     CDS             21690..21971
                     /locus_tag="PPY92_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDJ2624503.1"
                     /translation="MTYHERVLALRAESKRTAFDFRFEDLFSKEEWLSMSLAERQKAE
                     KAFRHEVKNMDDVRMPFSSVHDAQVKLYNVVYSYNGIKRNFKQVENEGF"
     gene            21907..22092
                     /locus_tag="PPY92_000480"
     CDS             21907..22092
                     /locus_tag="PPY92_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624504.1"
                     /translation="MLYILITALNVILNKLKMKDSNIISFIYSRHDRILYVNLVGNRF
                     KNNISMTKATSNIGRGF"
     gene            complement(22231..22422)
                     /locus_tag="PPY92_000481"
     CDS             complement(22231..22422)
                     /locus_tag="PPY92_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624505.1"
                     /translation="MRKEIEALIFSDVSSYDIYVNTGVNQGLIGDIKDGYLTIDSMPY
                     IDAERLYHFAMERKSLVTN"
BASE COUNT         7064 a         4266 c         3314 g         7815 t
ORIGIN      
        1 agtcttgcgc tattataaaa ttaaaaagtg tagagataac aacgcattaa cgtcgctatt
       61 ttccctacac ttaaatacta gtttctttaa ttaacttacc attaaagtta ttcagatatt
      121 gaatattcat taatcataat gtgaccatgc cgatagtatt aatacttctt tatttcgatc
      181 atctacggta tagacgaccc tgtgctgatg gttaattctt cttgaatatc gctctaaata
      241 tttaggctct aatttttcaa aagattgtgt tattttatac ggatcatttt ttaaagtctc
      301 aacaatttct aaaaatgact tctttaaata agaatgtttt attttcctta aatctgattt
      361 cgctgaattt ttaatcttaa ccgtgtaatt gctcattaaa gattatccca atcaatatca
      421 tctatatttg ttgtaccact attatctttt tctctttctc ttactttgtc cattgtacca
      481 gtagattcaa gatatattgt ctcttgtata cttttccaat cttctaaacc tataatcaca
      541 gcattatttt cggcattatt gccactaata taaattggtt cgtgattatt attaacattt
      601 tttagtaatt gataaaaatc ttttctcgct tctgtagggc tagtgataat catataaacc
      661 ctcctttgtt ttctgttacg ctaattatag cgtacgcact tgagtacgtc aatattgcca
      721 acgagttcat cgtattctta atacctatat gctatcgtac acaagctttt gttcttttct
      781 tccaacatac cgtctataat gctcaaaaag aaatcagcag tggaacacac tacgttccct
      841 accgctttat catttttcag gcttaaatat ttttaaaatt ttgtgaggaa tccgcttaaa
      901 aaactcatat cttcaaaaaa aatgacaaaa tttaaatttt tgaataacat cattttttag
      961 taaatgttat aatttcttta taatacaaat atcgaaagaa ggcgactgac tttgaaaaaa
     1021 ttatgttcat taattgtagt agcattaatt tgtatcattg cattatcagc ttgtggtaaa
     1081 gaacaaacca aaacatatga aggtgatgtt agtggtaaac atgtactaac ttctatcaca
     1141 tataaagatg ataaggtttt aaaacagtct acaatcaaca caattaaata tgacgatcta
     1201 ggtatggata aagacgaagc caaaaagtta ttcgccaaat ctgaaagtat tttcaaagac
     1261 cttaaaggcg taaaatacaa agtagactat aaagataaaa aagcaattga acacttagac
     1321 atagattaca cagaagttga catgaaaaaa ttaaataaac gtcttggtgt ttcaactaaa
     1381 gaaaataaag atattagttt tgaaaaactt gaaaagcaat taaagcacag aggtttaaaa
     1441 gaaaaagata aaatggacga caaatagttt ataacataaa aatgccctca gataagacta
     1501 aggatacaaa ccttaattca tattctgagg gctttaatat ttgaaattct tgtgtgacca
     1561 gcatccacta ctaatataaa attatttgca gtaacactaa aatccgctgc tttcattttc
     1621 tcgaaataga taaatcaact aatcaatttc aatttataat catgtatcgt ttgtaactca
     1681 ccatcgactt ttcgatatac aatatgatca gcagtaattt ctgtaggact ggatacgcca
     1741 acagctgctg caatattgaa taagccttca tgcaaacttg ttacatagtt tgtgacacga
     1801 tattgctttt ctccaacaat caatgctttt tcttttttcg catctgtcgt tgcaacacct
     1861 acaggacatg tattcatatg gcattgttga ctcataatac aaccgacact aatcatcatg
     1921 ccacgtgcga tatttacaaa atctgcacct aaacctagtg caatcgcaat tttatctggt
     1981 gtcactaact taccagatgc cgccaatttc actttatctc gaataccata tttttctaac
     2041 atgccagaca caataggtag agctgtaaat agcggtaagc caacaccatc ttgtaattct
     2101 tggaatgttg caccagtacc accttcacca ccatcaatcg taataaagct tggatactta
     2161 tctagttcca ccatcgtacg tacaagtgtt tcaatttctg aaactttgct tactacaatt
     2221 ttgaatccta ctggtttttg acctaattgc tgcaactgat cgacgaaacg aatcaaatct
     2281 tcagcattat gaataaattc gtaacggtta ggtgaattga ttgttttata aggttcaaca
     2341 tttcggattt tagcaatttc ttcgtttacc ttttcagctt ccatatgacc accacgagtc
     2401 ttagcacctt gtgccaactt cagctcaaat gcgcgtacgt tagataactg tgcgacctct
     2461 ttaaataaac cttcactaaa attaccttct ttatcacgaa caccaaataa accgggacca
     2521 atttggaaaa tgatatcccc attacctttt aaatgatatt ctgataagcc accttcacct
     2581 gtattcatcc aagtgcccgc tttagctaga cctttagata aagctgtaat ggcatttttt
     2641 cctaaagcgc cataactcat accagattgt cctacgatac gttttaaaat aaatggatgt
     2701 tttaaatgtt cacctaattt tattgcatgg tcatcactta agtaatacgg atcaatcttt
     2761 gtcggtacac gatattcttc acgactaaat aaacgctcat tcgcgatttt ataaatgaat
     2821 gttgataaca atgttgtatt atctactgaa atctcattac gttgcatcgg aaacattgtg
     2881 ttctgtatgt aaaagccgtc ttgataatct ttagtagtac cgaagctggt catacgagag
     2941 ttatattttc cagccaaaac gatattttta taatcattac gtgaaaaagg tttcccttca
     3001 ttatccccag aaaataaata ctgacgtaat tccggtccca ttttttctga aatatatcta
     3061 atacgtgcta gtaaaggata attccttaat acactatgtt gtgattgtct tttatcttta
     3121 attaaccaaa taagcccgat aacaataacc gtaagcagga agcctacaac gataatgtta
     3181 actataaatt gcatgactgt aagaaacgtc attacaatac ctccccccaa aatttcaatt
     3241 caatatttat gatacacctt acaaaacaaa acacaatgga agcgcttcat tttataaaac
     3301 aattttatga tatgtttttc attttaaatt ttaatgtata aaacatacaa tacaaagtaa
     3361 tatgtgctaa agtatctata taatacaact atttaagagg tatactatgt caaatacaaa
     3421 taaacattac atagaagaag aatacgctac tgaacaatcg cgttttttca aacgtgatat
     3481 tggatttatt ttcctttata tatttttggt taacatcttg ccaatcatta tcgtcttttt
     3541 aacttttggg ttagtagctg tattcactca acaagcccca tggggtttct cttttcctat
     3601 ttcaatgaac attagctatt tactcgcagt tattatttca ctagctattt ttcgtcggat
     3661 gcatgaagat gcatttgttc cgatgatacg tacgcaaatc attgccagca ccaaatataa
     3721 atggcatatt cttatatcat ttacactaag tgtcgtactc tttttacttt taaattattt
     3781 cgatattttc gcactaaatg tagatgcctt tactgtcttt gacatttacc cgctcattag
     3841 agattatgat tccatcagtt taatagtaat gttgtggtta accattatat taaatacttt
     3901 gatttgccaa cttattttcc gtcatatttt aattcaagaa ttaagcagat taataccttt
     3961 aaagattaca atagtattat ccgttatcct tgaaacactg tactattatc catacattaa
     4021 tacatggtgg gaattcattc cagcattgat actagctagt agtgcgactt acttatattt
     4081 aaagtcaaac cgtaatttta tggtgagcta tttctatcaa ttagcagtta tactcgtttt
     4141 gcatattttc atgtaaaatt tttcagagta ttatccatta tccatacaca tcaaaaagca
     4201 cacttaaaag tcagtatcac aactcacttt taagcgtgct ttatttagat atactgtcgt
     4261 tctaaaaacg agccatatag agagcctgag acataaatca atgttcaatg ctctacaaag
     4321 ttatattggc agtagttgac tgaacgaaaa tgcgcttgta acaagctttt ttcaattcta
     4381 gtcaaccttg ccggggtggg acgacgaaat aaatttttcg aaaatatcat ttctgtccca
     4441 ctcccatgaa tcgccatgct caatatacct tttaaaattt aaatattcat taacttaata
     4501 tgctttaatt cacttcatta aagactactc gctggacgtg gtgttggctt tttcaagaaa
     4561 ctcataatca ttattacaat gactaacata ccacaaatta gatacatcca tttaaatcct
     4621 actaaatcgg aaataggtcc catcaatgcg ccacctaacg atatacctaa gtctgcacag
     4681 gcaataaata aacctaacaa catattacga cctacttttg gtaagacgaa gcttaagtat
     4741 gatgttaatg ttgggtacac cattgcttgc gtcattccta ttaatatcgc actaccatag
     4801 aaaataattg cacctacttg cggaccaaat gccactacaa atgacgcgat tactaataat
     4861 gatagtacag ataccatata tttaggatgc cacataccat ctgacggaat gtatttcctt
     4921 aagtaaaatc ttgccgcaac aactgcgatg gcctgtattg tcaaaaagat tcccgcattc
     4981 gcgaatccta aactcactgt gtataacggt acaaatgtac taactgcacc aaatacaatc
     5041 gatgcaacaa tcatgataat accactgttc aacaactctt tatttttgaa aaattgcgca
     5101 aaaacagtta cagcgttaaa cggcattttt tcaatcttat ctgacgtatc gggttcctgt
     5161 tcagcaaagg tcacgcgata gccaaagaat gttgttgtta atgcgataaa gataatgaca
     5221 attgcaaata gtgaaatatt atttgcattc caaataccta cggcaactaa tggtccaatt
     5281 aagtttggaa tcgttgaaaa tagcgagtac aatgatacac cttcagaacg atgttcctct
     5341 ggtaatgcat caataatacc taactgtaaa gacatagaaa agaatgccgt acacacacct
     5401 tgcataacac gtgctacgaa ataaccttct aatccagtaa aaccataaat aattaaagct
     5461 atggcattga tgatcaaaat aattcttaaa actttaatgg gaccgacacg agcaataatt
     5521 tgtcctgccc atggtcgaaa caccattgct gttaacatgt atgcccccat aacgataccg
     5581 ataaccgtat tcgttgcgcc taaatcatgc cctcgtaacg gtataaatac gtttaagatt
     5641 gcattggcac taaagaacat caatgttaat atatataatc tcaaaaaggg ccaagccatt
     5701 gcacctttca ttatctcact ccttagaatg atagttgttt ttctattaat tctcttgtat
     5761 aagcattgtc acttttatgc aatgcgcttg tcggaatttg ttcttctatt tttccgtttt
     5821 taaaaataat taattgatca cataaatacg tggcagcttg aatatcatgt gtgataaaaa
     5881 tataactcaa ctgacgcgtt tcacgtaaat gaatcaataa atctaatatt tgtgtttgaa
     5941 ttgacatgtc gagtgaacta atggcttcat caaacaaaat atatttaggg ttaatacata
     6001 ttgcacgcgc aatcgcaaca cgctgcgctt ctccacctga taacatatta ggatatttat
     6061 ccatgtatgc cttagataga ccgacttctt ccaacaatgt aattgcttgg acttccataa
     6121 cttctttagg ttgtccatca cattgacaca tcacttcaaa taagatttct ctaacagtct
     6181 gaaatgggtg taatgatgac gtataatctt gaaatacagc accaatttga tgacgtctga
     6241 ctttcttctt atgcatcggt agatcattta aggttacaca gcctttatcc ggtttctcaa
     6301 tacctaatat catacgactc aacgtcgatt taccgctacc actttctccg ataatcgcaa
     6361 tcgtcgcacc gattggacac tcaaatgaca cacctttcac gataggtgtt cgacgacgct
     6421 taaaaacatg tgcgctttga tatgattttt caacatctgt aactttaatc atgtacatca
     6481 cccctcatca catgtttaaa atgatcatta atcttcttct tcgttgataa taaatacttc
     6541 gtataaacat gttctggatg atgcaagact gattcacgtg taccatgctc tatcagttga
     6601 ccatttttca tcacaacaac acggtctgca atcttgttaa taaccgttaa atcatgtgaa
     6661 atgaaaatca tcgcacagtc aaagtgtttt ttaatatcta taaatgcttc cagtacatca
     6721 tattgtgtaa ttgtatctaa agccgttgtc ggctcatcag caatgattaa ctttggtttc
     6781 aaagctaacg ctaaagcaat catcaatcgc tgtaacattc ctcctgataa catgtaaggg
     6841 tatgatttta atatacgttt aggatctttc aaacttaaat aatccatata ttcaatcaat
     6901 gtcttttcaa tttcttgtgt agacattgac gtatgtactt tcatagtctc aaacatttgt
     6961 ttaccgacag tagttgatgg gtcaaaggca cgactacctt gttgcatgac catcgcaata
     7021 tctttaccac ggtacttttt ccgttgcgat tcagataatg acaacattga tgtaccatca
     7081 aagataattt cacctgtcac cccgagtcgt tcgggattca aaccaataat cgatttgcat
     7141 gtaattgatt taccgctacc actttctcca ataacgccta aagtttcacc cttagttaat
     7201 gtaaaattca catcactcac gagtggttga tctgtccagg tatctgtaat cgtcaaatgc
     7261 ttaactgtta acaatgtcat gattgcacca ctcctttttt cacagaacga agtttatctt
     7321 tagaagagat gcggggatca atagcaattt gtaaagcatc ggataagaag ttaaatgcca
     7381 tcactataat cactatggca atacctggcg caaacatcat ttcaggatgt gtaaacatca
     7441 cttttctagc ttcgttaagc atcatgcccc actctgcagt aggcgctttg acacctaatc
     7501 ctaaaaatga aaagccagat atttgcaaga tcattgaaca catcgaacta ctagagatga
     7561 tagcaatatc tgctaatgtt aacggcataa tatgtttgtg aataattttc atatcattca
     7621 taccgattgt tttagcaaat ctgacatggt cagaagcagt gtactgcata acacttgtac
     7681 gtataacacg acagaaccat gcccaacgcg tcaaaataaa tgccatgata atattttcgg
     7741 cacccattcc aaacaatgca attaatgcta acgttacaac ataacttggg aatgccaaca
     7801 taacatcaca cgcacgcatg attaaggcgt caacaaaccc ttggaaatat cctgataaga
     7861 atcctaaaat agatccaata agtacagaaa caaatagcgc aacaaagaca tataacaaac
     7921 ttggtctaat cgcataaatt aacctagtta aaatatctct acctaaatgg tcagtaccta
     7981 gtagatgttg aaaactgatg ccagcaaatt tgtttgctgt atcgatatgg ttaggatcat
     8041 aaaatgtcac aagtggtgct gctaatccta aaaagacata taatacaata atgcctaaag
     8101 caattactgc acctttatct tgtaataatc gttttaaaat tatcatcgtg cgccctccct
     8161 taatcttgga tttaatagcg cattaatgat atctgctaat gtattaaata caataaataa
     8221 taccgctaca attaatacat atgcttgaat gactggaaaa tcgtgttcaa gtattgcttt
     8281 taaacttaat tgacctagtc caggccatgc aaagatatac tcgataacaa ctagtccacc
     8341 cattatcatt ggtatagaca tacaaaagat tgataccgca acttgtatag cattacgcaa
     8401 cacatgcaac attaatgtga tagatttcac accgcttgct cttaaataaa gtacataatc
     8461 ttcatttaat tgttccacca tcgagcgtct aacatttcta aagtaaatac cagcataggc
     8521 aatcgtaata acgatcactg gcaatatgta actttctggg cctgttaatc cagaagtcgg
     8581 caatatgttt aacttcactg aaacgtaaat aataagtatt gaagctatcc aatatgatgg
     8641 taatgcagtt agaaagaaag ccactgaacg tatcgcacga tcagtgaact ttcctctttt
     8701 taatgcacta actacaccta aaataattga tgtaatcatc accataacac ttgaaattat
     8761 tgttaatttc aatgtgttca taaatgctgg gccaatgcgt tcagcaacag gatcccctgt
     8821 aatgtaactt gtaccaaaat taaattgcat cgcttcaagt aaccagtttt tatattgaat
     8881 taataatgga tcattgaaac cgtacttttc attcgtttct gcaatcaact ctggtgttac
     8941 atttggcgtt ccttgtgcat gtaaaattgt cacagctgga ttttcatttg taatatacgt
     9001 caatagaaat gtcataaaac ttactacaat cactaatgga aacatgagcg caatacgttt
     9061 taagataaat ttgaacatct aattgctcct ttatttatac tgcatttcat tgaatggtaa
     9121 ttcatactgt gattgtgtga atgatacttt ttctaaatct ttcggcgcaa caactgtcat
     9181 actaccgtgt gaaataggga taaagatacc ttcatcatca atttgtttca aaatgttttt
     9241 ataagcgtct gaacgctctt taccgttttg gattttaaat gcgtcatcaa tgctgttgta
     9301 tattttatct ttgttctcaa tgcctgatgt tgcactttca taaccatttt tcgctttaaa
     9361 tgctgcaata gtactttgtg gatcgtacaa taatccccaa gtttggttga acattaagtc
     9421 ataatcacca gaagtacgac gttcagcaat tttatctgat gtttcgccat tgatgtttaa
     9481 cttaataccc attttcttaa attctgcttg taagtattct gcttgttctt tttgacttga
     9541 agaacctttg tcatagtaca ttgccatttc aaggttttta ccatcttttt gacgaacatc
     9601 actgtcttta cctttcttcc aaccagcttc atctaataat gattctgctt ttttaaggtc
     9661 atacttacgt gttggcatat cgaaattaat gtctgttaca tttttcgcaa ataattgagt
     9721 tgctggtttt tcttgaccat ctaaaatttc tttggcaatt ttatctctgt ttaccatatg
     9781 accaatcgct tgtctgactg ttttgtcact gacagcgtta tcttttttac cagaattgac
     9841 aactaacatt ttcgtattca taggttgact acgcttaact tgatagtcac ctgtatcttt
     9901 caattgtttt aaagagtctt tgtctaagct atccgtacct ctatcatctg tgaaggcaaa
     9961 gttcgtttca ccttttttca ttgataggaa tgctgtttca ccagcaggca ttacttttgc
    10021 ttgtactttg ttaagtttag acttttcgcc ccagtattga tcatttttgt taaagtctgc
    10081 agactcatct tttttgtgtt cacctaattt aaatggacca gtaccatcga actttttaac
    10141 gccatctttt gttgtaccgt ttttaaagtc ttttggagac acaaatacat atggacgagg
    10201 catcgctaat tcagccaatg caggttgata tgcttctttc aaattcaatt caaccgtgta
    10261 cttatcttta actttaacat tgtcaattaa tgttgaaatc tttaaccaag aatgcaattt
    10321 tttattttgt tgaaccgcat caatattttt cttaactgcg tcagcatcaa atgttgttcc
    10381 atcatggaat ttcacatcat ctcttaaatg gaatgtataa gtcttcccat cttcagacac
    10441 atcccatttt ttagctagta aaggcttaat accatctttc gtgttacgta caagcggctc
    10501 gtatatcata ctttcagcag acattgatcc accgtaaaca tgcggattca tatcaccgat
    10561 atctttaacc gtcgtatacg ttaattgctt gttttctttt ttctcctcta aacctttatt
    10621 accgccacaa ccagttaaaa ttagccctga tgcaagtaac attgcactca ttttagttag
    10681 ttttctcatt tgcttttcct ctttctaaat tgataagttg cattacttaa tcaaatcgta
    10741 atgattattt ttctcaatta tttgcttatt tttatgtaaa tgtcaaacat aattcaataa
    10801 tttttttcta ttataaaact tcaaaccctt attatgaaag cgttctattg atttccaaaa
    10861 attttgtgac gagtgcctta tctccttcaa atagatttgt attaaattga gaagatagtt
    10921 gttgatcttg gtgcatatcc ttgtacgcct ggcaacttgc ttcatagcga gttaggaact
    10981 gatcaatcat tggcgttttg atacctaaca ttttcccaat atgctgaatc atcgccgttc
    11041 tgtaataatc ttcacttggc attcttggaa tttgaacaac atcctgttca tttttataga
    11101 cttgcttaaa tggtacagct gaaaaatcaa agtaatgtcc gttttcgtct ggctgtgaaa
    11161 atggatcaat gaggattgcg gtgtatctta cataaagcag atattcttgt aagatatctg
    11221 gcaagatttc gaaatgctca atatcacctt catccaaagt ttcaggacgt actggataat
    11281 tttccttcac cataaattga agcaggttga ctgacggcac tctaaatgct tgtaaaatag
    11341 ccatcatttc cttccacatt aaacgcattt cacggattaa tgtcatcgtt atcggtcctt
    11401 caggaaataa cttatacaca taaaccggta catccgttcc ttcgaaaatg gctttcaatg
    11461 aaaagtcatt cataaatagt ggcgggtgca cataaagcga actgttacgt gtttcagcat
    11521 gcagtggcga ttcaacgact tctaattgaa tcttcaattg ctcagctaaa gcagagattc
    11581 gttgacacat tgttgagttt gaatgtgtcg atcccatgta caatttcttt tttacacctg
    11641 ttgtcaacac atgattaggt gcttctttat caacaatacg tgtatcgcca agataagttg
    11701 aaaatgaaat cacttcgata tcctgactga acttagacat aaattgttcc acaatcattt
    11761 gcgatccaaa tgtcggtgaa attaaaatga catgtttgac actttgcaaa gtttctaacg
    11821 acaattgctg tagtgtgtca taataagcat ctgctgtgca tgccatgaca accgtttcgt
    11881 attcaccctt aacgttttta acatctttat acaaacgatt aatttcaaac ttaccttcca
    11941 gatgttgatg cgcctcgttt tgtattttga cttcaaattg tttctctttt ttatacgctt
    12001 gatataagcg ttttgatttt tctgatgttg aggcacgtcc aaccatatca atctcataat
    12061 ctgattttaa atagcaaata ttcgctaatt ggattgcgac cggaccagtg cctatcatta
    12121 ataatttaga catccgtaat acctaccttt atagctgctt ttttataaag tgctatatca
    12181 aaaatttgct gtggtctcat atgtttattc acacattgcc acttatcttc cgctgtttct
    12241 tgtgacggat aattaaatat tgctttgatg ccatcaccaa agcgcattgc aactacgaca
    12301 ttttcatttg ttaaatcata taattcttct aaaatgctgt actttaaagg aattgtcgag
    12361 ctgaatatga tatgcgtcac atctttgata tctttaagtt cagatacctt ttgatccgta
    12421 attgttatat cttcatttgg tgctaagacg ttaacgattc tgcgccctag gtcaacggct
    12481 tgtggatcaa tatcaatacc gataactgaa gcacctgttt cttttgctac ttgaattaac
    12541 gtcattggat atgcacctga accaactaac aataatttgt cattgctagt gatactacat
    12601 tgtccaaact cttcatcaat acaatgttct atattgtcaa agtaacctgt ggtagacgcc
    12661 tgtccgtcta atagacgtct agctctaatg acttccactt gtttaacaca ctgcgctgta
    12721 atatattgca gtgattttat taattgtgct ttttcttcaa cgtcacgcca agcattaaat
    12781 tgttgttcat aaataggatt taaaataaat tcactatagt catccatcaa tgtttctaat
    12841 gcttcgatat attgatcgtc ttgtaataca cgctcgtaat gcgcttcaaa cttttccaaa
    12901 tattgttgta atatcaattt gatttcatta ttaaagttat tcattgtagg ctccttattc
    12961 tatatatgct tttcctgtag ctacagttgt aacctgtcct ttaattgaag tttgatatcc
    13021 caattgatga catcgctttg atgtcactaa aatactgccc cctggctgat gtactgtaaa
    13081 atctttgcat gcgtcattac gttgataatt attaaaaacc ccaattgatg ctgtaccaga
    13141 accacagcta ttttcccaaa ttaaactttg aatttctggt atatagatta atggctgtaa
    13201 aaattgacgt tgttcatcaa aaagcatcat acctactgtt ttatatttgt gactccattg
    13261 ttgctcacgc acaaatgctt caaccaaatg ttgaatttca gttgttactt gtttaactgg
    13321 aatcacataa tgtacatatg tttcataaat aatttctaat gctttccatg aatgattacc
    13381 catattaatt gttgttggca caacacgatg ggcttgtggc atttgaactt catagtattg
    13441 gcaatcatga attgcgcatt gcactaaatc cgaacagcca gataccttca ccttaaactg
    13501 ttggtcttta agcaaatgac tttcctgcaa atgatgtata tatgacatcg tcgcattacc
    13561 gcaaaattca ttaccgctca taactaagtg aaaatcattg ccatcatcat tttgtgttga
    13621 ttctataaag cctacctgtt cacaacatac atgtgttgcg gccatcaact gattggcgat
    13681 agatgcatat tcactagcat catgttttga atgaacaagt atcgtcatat tccccgaagg
    13741 attatactta gaaaattcta taacctgccg attcatacga cactccttta gatgtatttt
    13801 tagagcctct tttaccataa gtaattagag gctcttatgc agttggtgcg aagcaagcaa
    13861 ctgtattcct ctaaaactgt aatttttaga agatgaacct aatattttcc catctattag
    13921 gtaagtcatg ctaacccgca aaagcgaaaa ccatggctct tatgcagttg gtgcgaagca
    13981 aacaactgta ttcctctaaa ccttgtaatt tttataaaaa gaacctaata ttttcccatc
    14041 tattaggtaa gtcatgctaa tccgcatagt ggaaagcatg acttagtaat cggttgtttc
    14101 atcaacatgc aaccgtaaac cttatttaaa gttgcttctt gtggctctac aagtgaatgc
    14161 agttgtccgt taacttcatt cacttagatg cttttactgt tttcacctca actaaaactt
    14221 caaaaaagtt gcagaaaaca aatcgtaact attacgattt gattatatac tatattagtt
    14281 gagaatcatt gtcaataaag gttgtaaatg aatgcacatt tatattttga taatctttcc
    14341 ataaacatcg tattctcagc acattcaagt cttttgaaaa tttttatcaa acaaaaaaac
    14401 cagcttcaac gtaaaatacg ttaaaactag ctttaagcaa tacacttcat caaataaaca
    14461 aacatttatt tttcaaaggt taataattcc ccgtcatttg gtacaacaac tctattacct
    14521 aagtcgttag aatttataaa ttcattcagc tctttacgag ataaatgcca atgattgacc
    14581 gcttccatat gcacaacaac aatttgcgca agtggcaatg tattagcaac ttttaacaca
    14641 tcatgttcat ccataactaa cggcccacct tcaaagaatt gatttgcacc accattaaga
    14701 acaacaacat ctggttgata cgtttttaat gctttttcaa ccccttcaaa ccaaactgta
    14761 tctgctgcta agtataatgt cggctcttca gaatgtttta aaatcatgcc gtgaacatgt
    14821 cctgtcatct tcaaaatttc accatgacca tgttgagcat ctgttttaat caactcaatt
    14881 tctccaatgt gtgtcacatc attaaataca gaaacatttg taaacccata acgctctact
    14941 tcactagcat cttcttcatt ttgtgcgtaa attttaatat ctttcggcaa tacttcaatt
    15001 gctttcggat caaaatgatc aatatgcaaa tgcgttaaaa tcactaaatc gatgtcattt
    15061 ataatgtctt caattgccat gggtagttct acaagtggat ttttttgatc ttgtctcggt
    15121 gaatttggaa aagggtcaaa tgaaccttta tcacttaaca ttggatcaat caaaatgcgt
    15181 ttacctccat actcaatcac ggccgtggca tttcttatat gtctaatatt catgttaaat
    15241 ccccctattt catatgtctg tcattgcgac aatatgcata cattgcctac ttcatgccaa
    15301 attcttttcc actggatatc gattttaaat aattattcca atgtccgctt ccacgaatca
    15361 tcacttaaca tctcgccagg ccatgtgtaa gccatcacac caccatcaat tcgaatcgtc
    15421 tctccagtta tgaatgaact gtcgtcagat gctaagaaga ctactaattt agcaacttct
    15481 tctggtttac ctaaacgtcc cagcggagtc atccattttt gattttctct aaatgtttta
    15541 cctgcatcat cctcactcgt acctgtcagt ttatctacta acggggtttc aattgtacct
    15601 ggtgcaattg cattggatcg aatgccatca cgaccatact caattgcgat tgattttgta
    15661 aaattaatca ctgcgccttt cgcagcatta tatcctgagc gatacaaatc ttctgcctgt
    15721 cctgaaaatg aagacgtatt aacaatagag ccaccttgat tcatcattaa aggtaacatc
    15781 attttcgtca ttaaaattgt cccacgcata tctacattca taatcttgtc atacacatct
    15841 attggatact catgaattct accagccgca ttatccacac cggcattatt aaacaacaca
    15901 tcgattcttc caaactgttc ctttatgtca gacacaaagt ctaccacttg ttgttcatct
    15961 gaaatatcca cattatacgc cttcgcattg tcaccgttac ttttaatttt atcgacagtc
    16021 tccgataccg cttcagctat gtctaccgcc aatacatacg caccttcttg agctaaagcg
    16081 attgcagaag cttgtccgat acctgtactt gctcctgtta cgactgcaac tttattttcc
    16141 aaacgtttca ttcagtcact cctatacaac atatcttaaa cttacagtcc ttattttcca
    16201 ctgttcaatg cttcataaac ctaatagata agtctccagg cagtcttagc tactctctgc
    16261 tatacaattt ctattgaata actcaaaata tttaacaata ttagtctttt cattttctga
    16321 aaattgttac ttttgagagt attttttgtt acactttaaa tatttaacaa aggggggaat
    16381 cattgatgac atcaaaacat caacaaaagg ggtctatcgt caatcgtttc ttgaatagtg
    16441 ttgaaaaaat tggaaataaa ttgccagatc ctagcgtctt attcttttta atgtgtgtgg
    16501 gcttagccat tatgacgtgg gttatctcat tatttaatgt atctgttaag catccaggta
    16561 cgcatcaaac catttatatt aaaaatataa ttagccatga tggatttacg atgataatga
    16621 atgatacgat taaaaatttc tcagagttcc cagcattagg cttagtacta gcagtgatga
    16681 ttggtattgg tgttgcagag aaaacaggat acttcgataa gttaatgatt tctgttgtga
    16741 atcgcgcacc acgattttta attttaccga ctattatatt aattggtatt ttaggtagta
    16801 cagccggcga tgctgcgaca attatcttgc cgccgcttgc agcaatgctt tttattaaaa
    16861 ttggctatca ccctattgct ggtttaacga tggcatatgc ttccgctgtt ggaggatttg
    16921 cagcaaatat agttgttggt atgcaagatg ctttggtcta ttcatttaca gaaccggcaa
    16981 cacgtatcgt ttcagattct attaaaacaa acgttgctat gaactggtac tttatcgcag
    17041 cgagtgtcgt tgtattactt cctacgatat tgctagttac aactaagctc attattccta
    17101 gattaggaaa atatgatgac agtttaatgc atgatgacca tgaagaagca tcttcacata
    17161 ttactgacaa agaagcacat gctttaaaat gggcaaatat cagtttcata gtcacaatta
    17221 ttttattaat tattacagct attcctgaac atagtttttt aagaaatgct aaaacaggca
    17281 gcttactaga cgatgcgcca ttaattaacg gtgtcggatt aattattctt gtcgtatttt
    17341 tagtacctgg attagtttat gggattttaa gcaaagaaat taaaaataca aaagacttag
    17401 gtaaaatgtt cggcgatgct gtaggatcaa tgggaacatt tatcgttatt gtgttttttg
    17461 cagcacagct acttgcgtat ttaaagtgga gtaatttagg aattatcgct gctgttaaag
    17521 gtgccaaatt attagaacat caaaacggta ttgtattgat actaggtatt attgtactga
    17581 gtgcaatggt caacatgtta atcggaagcg catcggctaa atggggtatt ttaggaccga
    17641 tattcgtacc aatgttaatc cttatcggct ttcatcccgc atttacgcaa gtcatttacc
    17701 gtgtaggcga ttcaattacc aatccaatta caccgatgat gccgtactta cctttattat
    17761 taacttatgc acaaaaatac gataaacgca tgaaactcgg agccttactt tctagtttaa
    17821 tgccgtattc aatcgcgcta agcattgtat ggacagtatt tgtcatcatt tggttcttat
    17881 taggtatccc tgttggacca ggtggaccga tattcgttaa ataacagtat tcaaagggat
    17941 aaattaaaaa tgccactata caagatttga cctgtatagt ggcatgattt gttatgatat
    18001 agcgttcgag atgaaactct gcatttatat gaacaattat agtctatcac taataatatt
    18061 tagtctgttc caaccgttaa tttggtttac aacaaagaca agatcaataa tttgttcttc
    18121 atcataaaat gattttagcc ggtcaataat ttcaaacttc ttaaagtctt tgattgaatt
    18181 taacatttct gcaaattcaa gcgtcacttt ttcttgatca gtaaataaat ctaaatgtct
    18241 aaaactcaag acttcatcaa ttttcatttg tgttacaccc aattccttta attctttttt
    18301 atggatatca acacaataat gacaaccgtt aatttttgaa attaataact taatcaatgc
    18361 actaagttca tcattaaccg gtgattgatg aataaacttt tcaccttggc tcattacaga
    18421 cattaactct ccattttgtt tcttgtaatt atacggcatt tgcaatctct cctttacatt
    18481 gtttaatacc aatccagtga tactacactt cgaacaatat tggatagaat ttaaaattct
    18541 gtataacatc ttgtcaatct atgctttagt taaaacattt agcgcttata tttaaaaaat
    18601 atataaaata tgaaaacaga ttgaaaggat attatacctt gctctaccct tttttataaa
    18661 aaatcacaca catttgagaa aatttgataa tgtcaataaa tcacgtcatc gccattgtta
    18721 ttcggtatat aatgttttta taatggctaa actattattt catttacatt aatggggttc
    18781 gcttgtgtta atgtactgta caaaactggg gaagtattgg tgatgatgaa gtgattgata
    18841 ttaattgcga aacaccttcg atagcaatga catagattat tggaagatta aatcaaaaat
    18901 aaagaagacc ctagtggcct tcttttttgt ctaattcttc tttgatttcg tcaggtgatt
    18961 ttaattgatg cgctggtttt agtaactcac ttaatctttc actttctatc atagcaccac
    19021 caaactgata attatgttct ggtgtagcat aagcagtaaa attatctttt ttgttttcat
    19081 tgatgtagcc ttcaattacg gcatcgccca tgggtcctct ttttaaactt gtgaaatgca
    19141 cagatttgat ggtattgggt tctttggtat tatgctggag atagagtgta atacgctcct
    19201 gttgttcttt atagtatatt gctttttgtt tttctttttc gtcatatttc acttttaaat
    19261 aaatgactga tgcaactata catatacata aaatgacacc aataattaca aaaacatgtt
    19321 ttttgttcat taaagtaact cctaacttta gtgactctac tttttattta gttctttttt
    19381 tatatcatct ggtgatttta attgatgcgc tggttttagt aactcactta atctttcact
    19441 tttaattaaa tcacctacaa attgaaagtt attttcaggt gatgcatagg caacaaaatc
    19501 atcttcttta ttttcattta tgtaaccttc aatcacagca cttcccatag gacttgtttc
    19561 caattttgtg aaatgtacag atttgattgt gttcggttct atagtattat gcttaagata
    19621 aagtgtaatg cgcgcttgtt gttctttata ataaattgat ttttgttttt ctttttcgtc
    19681 atatttcatt ttaaaataga tacttgcaac actaattaat atgattaaca taatactagt
    19741 taatattaaa attttctttt tcgacatcta tcaccagctc ctaaaatgtg gtggaatttt
    19801 atacagttta catatttatg atactacaaa acacactctt acaaagaaaa tctataattt
    19861 taatctaggt caaggaaaag cagccaaaag tgtttatata agatcaacga aatattgcgg
    19921 gtataaagtg gttaacagat aaatacagtg gaaattgtac taaaaattca acttttcatt
    19981 tttatatgta agtatgatta acttgaaaac aaaattaaaa cggccgcata gttttagcgg
    20041 tcgttctttt tagactctaa ttcctttttt atctcatcag gtgatttggt ttgatgtact
    20101 ggttttaata acgtgcttaa atttttactt ttgattaaac ttccaccaaa ttgataatta
    20161 tgttctggag acccatatgc aacaaaatca tcttctttat tttcatttat gtaaccttca
    20221 attactgcat cgcccatagg tcctcttttt aaacttgtga aatgtacagt tttgatggta
    20281 ttgggttctt tagtattatg attaagatag agtgtaatac gctcctgttg ttctttatag
    20341 tatattgctt tttgtttttc tttttcgtca tatttcactt ttaaataaat gactgatgca
    20401 actatacata tacataaaat gacaccaata attataaaaa catgtttttt gttcattaaa
    20461 gtaactccta aaatgtggtg gaaaatgaaa tttttttgta atctgtaatt acgaacatta
    20521 tagattataa accataacac taacatcgtc gccttcatta acttgattgt taaaatcacc
    20581 aattactgag aattgtacaa ggaataattg cctattatgc cctcatgtca ttattgcctt
    20641 actaacatta acaaaatcga tagctattac attaaatgtc tatctcccac acctcaaaca
    20701 cctttttata caggacgcac tgtaatttca ttcacgttaa catgctttgg ttgtgttaat
    20761 gcatacaata ccgcttctgc aatatcttgt ggatcaagct ttttacgatc acttggatta
    20821 tatgcggctg ttattgctgt atctaccatt cctggagaaa tgcttgttac tttaacgcct
    20881 gtctttgcca actctttttc taatccttga gtaatagtgt gaactgctgc tttcgtcgca
    20941 ctataaatcg tactactttt cgttacttca aagccagaaa tagatgcaat gttaataaga
    21001 tgtccacttg attgttctaa catagttggt aatgcagcct gtgccgtata taaagtgcct
    21061 ttgatattca catcaatcat actatcccac tcatctactt gataatcagt aatcttagac
    21121 gataacattt gtcccgcact attgataaca atatccaaac caccgaatgt ttgttgtgca
    21181 attttcatca attcatcgac ttcttcttta ttcgttacat ctgttggcac taccttcaca
    21241 ctatcttgag ctaattgatt cgccacattt tgtagtttat ctttatttct accggctaag
    21301 acaactttcg ccccttcttc atgtagtaat gttgcaattg cttctccaat accactacct
    21361 gcacctgtaa ctactgctat tttatctgtt aatactgtca taatgatcga ctcctttgat
    21421 tctttttatt ttttcagggt aaatcataaa tacatattac ttttaaaaag cgtatcacaa
    21481 ttcatataac ggtcataata actcgcttca ttttcataga taaattacat tacaagccat
    21541 tcgaaatata caattaatcg ttgcttatat tttttatttt taaaaatgtt gaaaaatcgt
    21601 cacttcttta ttgtaaaaac attatattag tactaaagtt aatactgtga tttatcattc
    21661 gattgaatga ttagagggag gaattaaacg tgacatatca tgagcgtgtt ttagcattaa
    21721 gagcagaaag taaaagaacc gcatttgatt ttcgattcga agatttattt agcaaagaag
    21781 aatggctaag tatgtctctt gcagaaagac aaaaagctga aaaagcattt cgacacgaag
    21841 ttaaaaatat ggacgatgta agaatgccct tctcaagtgt ccatgacgcc caagtaaaat
    21901 tatataatgt tgtatattct tataacggca ttaaacgtaa ttttaaacaa gttgaaaatg
    21961 aaggattcta atatcatttc gtttatatat agcagacatg atagaatttt atatgtaaat
    22021 cttgtaggta atcgttttaa aaataatata agtatgacta aagccacatc caatatagga
    22081 cgtggctttt agtatcgttc cagatttgtg gggagttagg gtttacttaa ttaaagggat
    22141 ctgccctcaa ttgctcacca acgagggcaa gttacatcaa tcacacttca attgccgccg
    22201 actaggggta gtaatcattg gcaataagaa ctagttagtg actaacgatt tacgttccat
    22261 agcaaagtga tacaaacgct cagcatcaat gtaaggcata gaatcaatag ttaggtaacc
    22321 atctttgatg tcaccaatta atccttgatt tacaccagtg ttcacgtaaa tatcatagct
    22381 acttacgtct gagaaaataa gcgcttcaat ctcttttctc ataatcatca ctccaatgtt
    22441 tatatattat ttatataaa
//
LOCUS       DANBWH010000018        22438 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.018, whole genome shotgun sequence.
ACCESSION   DANBWH010000018 DANBWH010000000
VERSION     DANBWH010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 22438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22438
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.018"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            119..1498
                     /gene="uhpT"
                     /locus_tag="PPY92_000482"
     CDS             119..1498
                     /gene="uhpT"
                     /locus_tag="PPY92_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HDJ2624506.1"
                     /translation="MNFFDIHKTPNKGIPLSVQRKLWLRNFMQAFFVVFFVYMAMYLI
                     RNNFKAAQPFLKEEIGLSTLELGYIGLAFSITYGLGKTLLGYFVDGRNTKRIISFLLI
                     LSAITVLIMGFVLSYFGSVMGLLIVLWGLNGVFQSVGGPASYSTISRWAPRTKRGRYL
                     GFWNTSHNIGGAIAGGVALWGANVFFHGNVIGMFIFPSVIALLIGIATLFIGKDDPEE
                     LGWNRAEEIWEEPVDKENIDSQGMTKWEIFKKYILGNPVIWILCVSNVFVYIVRIGID
                     NWAPLYVSEHLHFSKGDAVNTIFYFEIGALVASLLWGYVSDLLKGRRAIVAIGCMFMI
                     TFVVLFYTNATSVMMVNISLFALGALIFGPQLLIGVSLTGFVPKNAISVANGMTGSFA
                     YLFGDSMAKVGLAAIADPTRNGLNIFGYTLSGWTDVFIVFYVALFLGMILLGIVAFYE
                     EKKIRSLKI"
     gene            complement(1857..2615)
                     /locus_tag="PPY92_000483"
     CDS             complement(1857..2615)
                     /locus_tag="PPY92_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDJ2624507.1"
                     /translation="MFKVVICDDERIIREGLKQIIPWGDYHFNTIYTAKDGVEALSLI
                     QQHQPELVITDIRMPRKNGVDLLNDIALLDCNVIILSSYDDFEYMKAGIQHHVLDYLL
                     KPVDHAQLEVILGRLVRTLLEQQSQNGRSLASCHDAFQPLLKVEYDDYYVNQIVDQIK
                     QSYQTKVTVSDLIQHIDVSESYAMRTFKDHVGITIVDYLNRYRILQSLQLLDRHYKHY
                     EIADKVGFSEYKMFSYHFKKYLQMSPSDYCKQAK"
     gene            complement(2608..4164)
                     /locus_tag="PPY92_000484"
     CDS             complement(2608..4164)
                     /locus_tag="PPY92_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDJ2624508.1"
                     /translation="MTAYKPYRHQLRRSLFASTIFPVFLVIIIGLVSFYAIYIWIEHR
                     TIHQHVDESQSSLHHTEKQIQTFITQHNNSFQELDLTNHHDVTATKRELLKLIHQQPA
                     TLYYELSGPNQFITNNYEHLNTKNMYLFSTHQLKFNNSTYMLKIYMANTPRLSEIKKD
                     SRQFALIVDQYDNILYANDDRFTIGEKYRPQQFGFMNESVKLNHADHRLIIYKDIHEN
                     IEDGITLLIVMAVVLVLLVIFGFISADNMAKRQTKDIETIIQKIYYAKNRHLGTYTPL
                     KNNSELEEINNYIYDLFESNEQLIHSIEHTERRLRDIQLKEIERQFQPHFLFNTMQTI
                     QYLITLSPKLAQTVVQQLSQMLRYSLRTNSHTVELNEELNYIEQYVAIQNIRFDDMIK
                     LHIESSEEARHQTIGKMMLQPLIENAIKHGRDTESLDITIRLTLARQNLHVLVCDNGI
                     GMSSSRLQYVRQSLNNDVFDTKHLGLNHLHNKAMIQYGSHARLHIFSKRNQGTLICYK
                     IPLSRGNVDV"
     gene            complement(4161..5129)
                     /locus_tag="PPY92_000485"
     CDS             complement(4161..5129)
                     /locus_tag="PPY92_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDJ2624509.1"
                     /translation="MKSKIYILLLFLIFLSSCANTRHSESDKNVLTVYSPYQSNLIRP
                     ILNEFEKQEHVKIEIKHGSTQVLLSNLHNEDFSERGDVFMGGVLSETIDHPEDFVPYQ
                     DTSVTQQLEDYRSNNKYVTSFLLMPTVIVVNSDLQGDIKIRGYQDLLQPILKGKIAYS
                     NPNTTTTGYQHMRAIYSMHHRVSDVHQFQNHAMQLSKTSKVIEDVAKGKYYAGLSYEQ
                     DARTWKNKGYPVSIVYPIEGTMLNVDGIALVKNAHPHPKRKKLVQYLTSRSVQQRLVA
                     EFDAKSIRKDVSEQSDQSIENLKNIPLIPKSKLPDIPHHKFLEMIQ"
     gene            5717..7966
                     /gene="pflB"
                     /locus_tag="PPY92_000486"
     CDS             5717..7966
                     /gene="pflB"
                     /locus_tag="PPY92_000486"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDJ2624510.1"
                     /translation="MLETNKNHATAWQGFKNGRWNRHVDVREFIQLNYTLYEGNDSFL
                     AGPTEATSKLWEQVMQLSKEERERGGMWDMDTKVASTITSHDAGYLDKDLETIVGVQT
                     EKPFKRSMQPFGGIRMAKAACEAYGYELDEETEKIFTDYRKTHNQGVFDAYSREMLNC
                     RKAGVITGLPDAYGRGRIIGDYRRVALYGVDFLMEEKMHDFNTMSTEMSEDVIRLREE
                     LSEQYRALKELKELGQKYGFDLSRPAENFKEAVQWLYLAYLAAIKEQNGAAMSLGRTS
                     TFLDIYAERDLKAGVITESEVQEIIDHFIMKLRIVKFARTPDYNELFSGDPTWVTESI
                     GGVGIDGRPLVTKNSFRFLHSLDNLGPAPEPNLTVLWSVRLPDNFKTYCAKMSIKTSS
                     IQYENDDIMRESYGDDYGIACCVSAMTIGKQMQFFGARANLAKTLLYAINGGKDEKSG
                     AQVGPNFEGINSEVLEYDEVFKKFDQMMDWLAGVYINSLNVIHYMHDKYSYERIEMAL
                     HDTEIVRTMATGIAGLSVAADSLSAIKYAQVKPIRNEEGLVVDFEIEGDFPKYGNNDD
                     RVDDIAVDLVERFMTKLRSHKTYRDSEHTMSVLTITSNVVYGKKTGNTPDGRKAGEPF
                     APGANPMHGRDQKGALSSLSSVAKIPYDCCKDGISNTFSIVPKSLGKEPEDQNRNLTS
                     MLDGYAMQCGHHLNINVFNRETLIDAMEHPEEYPQLTIRVSGYAVNFIKLTREQQLDV
                     ISRTFHESM"
     gene            7989..8744
                     /gene="pflA"
                     /locus_tag="PPY92_000487"
     CDS             7989..8744
                     /gene="pflA"
                     /locus_tag="PPY92_000487"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDJ2624511.1"
                     /translation="MLKGHLHSVESLGTVDGPGLRYILFTQGCLLRCLYCHNPDTWKI
                     SEPSREVTVDEMVNEILPYKPYFDASGGGVTVSGGEPLLQMPFLEKLFAELKENGVHT
                     CLDTSAGCANDTKAFQRHFEELQKHTDLILLDIKHIDNDKHIRLTGKPNTHILNFARK
                     LSDMKQPVWIRHVLVPGYSDDKDDLIKLGEFINSLDNVEKFEILPYHQLGVHKWKTLG
                     IAYELEDVEAPDDEAVKAAYRYVNFKGKIPVEL"
     gene            9066..10829
                     /locus_tag="PPY92_000488"
     CDS             9066..10829
                     /locus_tag="PPY92_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HDJ2624512.1"
                     /translation="MKRISKDIWAVFKLLYQNKGRFSINALLLQLIMIFISSTYLILL
                     FNMMLKVAGQSQLTINNWMEIISHPASVILLIIFILSVAFLIYVEFSLLVYMVYAGFD
                     RQIITFKSIFKNAFVNVRKLIGVPVIFFVIYLMLMIPIANLGLSSVLTKNIYIPKFLT
                     EELMKTTKGIIIYGTFMIAVFILNFKLIFTLPLTILNRQSLFKNMRLSWQITKRNKFR
                     LVIEIVILELIIGAILTLIISGATYLAICVDEEGDKFLVSSILFVVLKSALFFYYLFT
                     KLSLISVLVLHLKQENVLDQPGLEFKYPKSKRKSRFFIISMVLAVTCFIGYNMYLLYN
                     NTINTNISIIGHRGFEDKGVENSIPSLKAAAKANVEYVELDTIMTKDKQFVVSHDNNL
                     KRLTGVNKNISESNFKDVVGLKMRQNGHEAKLVSLDEFIETAKQSNVKLLVELKPHGK
                     EPADYTQRVIDILKKHGVEHQYRVMSLDYDVMTKLKKEAPYLKCGYIIPLQFGHFKET
                     SLDFFVIEDFSYSPRLVNQAHLENKEVYTWTINGEEDLTKYLQTNVDGIITDDPALAD
                     QIKEEKKDETYFDRSIRILFE"
     gene            complement(10993..11337)
                     /locus_tag="PPY92_000489"
     CDS             complement(10993..11337)
                     /locus_tag="PPY92_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN family protein"
                     /protein_id="HDJ2624513.1"
                     /translation="MTTQMKIKTYLVAGIKAALLDTTGIKLASKSETTSHTYQHQALV
                     DQLHELIANTDLNKLSYLNLDAFQKRDILAAHYIAKSAIRTKNLDQMTKAKQRLESIY
                     NSISNPLHSQNN"
     gene            11527..13503
                     /gene="coa"
                     /locus_tag="PPY92_000490"
     CDS             11527..13503
                     /gene="coa"
                     /locus_tag="PPY92_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylocoagulase"
                     /protein_id="HDJ2624514.1"
                     /translation="MKKQIISLGALAVASSLFTWDNKADAIVTKDYSKESRVNEKSKK
                     GATVSDYYYWKIIDSLEAQFTGAIDLLEDYKYGDPIYKEAKDRLMTRVLGEDQYLLKK
                     KIDEYELYKKWYKSSNKNTNMLTFHKYNLYNLTMNEYNDIFNSLKDAVYQFNKEVKEI
                     EHKNVDLKQFDKDGEDKATKEVYDLVSEIDTLVVTYYADKDYGEHAKELRAKLDLILG
                     DTDNPHKITNERIKKEMIDDLNSIIDDFFMETKQNRPNSITKYDPTKHNFKEKSENKP
                     NFDKLVEETKKAVKEADESWKNKTVKKYEETVTKSPVVKEEKKVEEPQLPKVGNQQEV
                     KTTAGKAEETTQPVAQPLVKIPQETIYGETVKGPEYPTMENKTLQGEIVQGPDFLTME
                     QNRPSLSDNYTQPTTPNPILEGLEGSSSKLEIKPQGTESTLKGIQGESSDIEVKPQAT
                     ETTEASQYGPRPQFNKTPKYVKYRDAGTGIREYNDGTFGYEARPRFNKPSETNAYNVT
                     TNQDGTVSYGARPTQNKPSETNAYNVTTHANGQVSYGARPTQNKPSKTNAYNVTTHAN
                     GQVSYGARPTQKKPSKTNAYNVTTHANGQVSYGARPTYKKPSETNAYNVTTHANGQVS
                     YGARPTQKKPSETNAYNVTTHADGTATYGPRVTK"
     gene            complement(13685..13792)
                     /locus_tag="PPY92_000491"
     CDS             complement(13685..13792)
                     /locus_tag="PPY92_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624515.1"
                     /translation="MCVRTRLVSSSSVRLSKAIIIAVIVVYHLDVRGLF"
     gene            complement(14090..15274)
                     /locus_tag="PPY92_000492"
     CDS             complement(14090..15274)
                     /locus_tag="PPY92_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HDJ2624516.1"
                     /translation="MQEAYIVAYGRSAAAKAKQGALFHERPDDVAAKVLQGVLKRIDG
                     KFNKNMIEDVIVGTAFPEGLQGQNIARTIALRAGLSDTVPGQTVNRYCSSGLQTIAIA
                     ANQIMAGQGDILVAGGVELMSAVPMGGNEPTNNPTLQYDDIGASYPMGLTAENVASQF
                     DVSREDQDAYAVRSHQRAYDAQRDGRFKDEIIPIRVNSVEYTNAGPKVHTNIFDQDEF
                     IRPDTTMEALAKLRTVFKADGTVTAGTSAPLSDGAGFVVLMSGDKVKELGVTPIARFV
                     GYKAVGVDPKIMGIGPAYAIPEVLSLSNLSVEDIDLIELNEAFASQTIASIKEVGLDI
                     SRTNVNGGAIALGHPLGATGAMLTARLLNEMGRRPDSRYGMVTMCIGVGMGAAAIFEY
                     VR"
     gene            complement(15304..17565)
                     /locus_tag="PPY92_000493"
     CDS             complement(15304..17565)
                     /locus_tag="PPY92_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HDJ2624517.1"
                     /translation="MTINKVTVLGAGTMGAQLAALFVNAGLKVKLLDIVVDKNDPNLI
                     AKKSYDKITDKKRPLLFDLNLVSHLTYGNFDDDLVNDDADLYIEAVKEDIEIKHAVWQ
                     QVLQHAKEDALFATNTSGIPINAIAQAFNEKDQERFFGLHFFNPPRIMKLVELIPTSH
                     TKESIILDVKNFAQNVLGKGVIVVNDVPGFVANRVGTQTMNDIMYRAEQHKLSIVDVD
                     ALTGQAIGRPKTGTYALSDLVGLDIAVSVIKGMQQVPEETPYFHDVKIVNTLFDNGAL
                     GRKTKQGFYKKDKETKARLVYDVEKQDYVPVSQPQLPILNEFNKDLVHNLDVIFNAQD
                     EAGLFLWETLRNNFYYSAINVPKATDDFRDIDRALVWGFNWKLGPFQLWDAMGYERVK
                     TRMEDELGDLPQWISDLDGGFYKQDETIEYATPVSHFVKDELWDKGDAKLSVTHDDQL
                     LLKLQSKNNVITDEFNDALVDAIDLLENDHYTSMVIYADGNNFSVGANLFLMKKAHED
                     GLVDDVVAQSIDKLHYSFNRLKYSLKPVVTAVQGRALGGGCELVLYSPIVVAASETYI
                     GLVEAGVGLLPSGGGLAEMADRILRTSHKFDDKQASMTKVLTNIAFAKVSTNAFEARR
                     YGYLRDTDTIIFNTAQRVEVALKRAKYEAETNYIPNPRHQYIALGEDFKALIQGQLDA
                     QRRGHFISDHDYHIALNIATILAGGDLPRNTFINQRYIQSLEKIGFIDLLKSKKSYER
                     IAHMLKTGKPLRN"
     gene            complement(17751..18962)
                     /locus_tag="PPY92_000494"
     CDS             complement(17751..18962)
                     /locus_tag="PPY92_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDJ2624518.1"
                     /translation="MTFEKETVLKTLFPEDVLSIAKGLTDGEVEFLQQVDSLLESNYR
                     ENINQHWIDATVPEDYFKDLGELNYFNNPLLYKDRPNAKMPSQLFQFFMSYLLARFDI
                     SLATLLGVHQGLGHNTFYFGGSKEQIAKYVPKLQSHELRTCFALTEPEHGSDVAGGLE
                     TVAERQGDTWVINGEKKWIGGAHVSDVIPVFAVNKKTGKPHCFVVRPEQDGVDIEVID
                     NKIALRIVPNALIKLTNVKVDEADRLQNITSFKDIAKILYSTRAGVAYMATGGMAGAL
                     RATLDYVTERKQFGKPISKYQLIQEKLAMMQGNLAQAMATCAQLANMQAHGEYDEVAT
                     STAKMMNALRLRETVAMGRGITGGNGILADDYDIARFFSDAEAIYTYEGTHEINALVI
                     GRALTGDSAFV"
     gene            complement(19074..20579)
                     /locus_tag="PPY92_000495"
     CDS             complement(19074..20579)
                     /locus_tag="PPY92_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HDJ2624519.1"
                     /translation="MNFDWIKTRSDFDDDKPAVIDHAKQTSWTYQQLNARADNMAHYL
                     TSQGVKKGDVIGIFAPNDIAILDLLFACFKTGAVFLPLNWRLNPKEIAAIVEDAQLKL
                     LFYAEKHLSSLTDIDQNLLHMDIDVAQYDEIVNPDYHQPFQATPVEPQDLAALIYTSG
                     TTGSPKGVMFSYESFVHNGANLELTYKFNSNYITIVSTPMFHVLGFNDTVLPVLMSGG
                     TLILQRYFNGEELNDMIAQYHPTFIIMIPTMYYSTLRASNFNPENFRAMDYIIQGGSQ
                     PLPSIQAAFKQYGINIINGYGLTEAPLVLVNTPENSKRKPMSIGKAVMFVDARILDDN
                     GEEVPTGEIGELAIKAKNVTPGYWNKPAETAKAFHGRYLLTGDLAKMDDDGDIFIIDR
                     KKELIITGGENVLPSEVENALAEHPLVDRCVVVGYDHPKYGESIAAAIILREDEPHYA
                     EILDQHMRSRLAGYKVPRMYVPVTHMPLNSTQKPDKLAIRQMMNDKVSQTL"
     gene            complement(20605..22182)
                     /locus_tag="PPY92_000496"
     CDS             complement(20605..22182)
                     /locus_tag="PPY92_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDJ2624520.1"
                     /translation="MEVTCLKQITWHDLQHIIKDGDVIGLPALAVANLPAEVLRAVLA
                     QHDTYHTPKDLTFILANDIHSLGAAPDLDDFIERRMIKRVIMSILTASSKTAQAMKNN
                     DIEAYFLPQGIIATHYRQSNQLLPGVITKIGLNTAVDPRYGGGKVNTRTTDDLVSLVT
                     INDETYLHYTFPSVDVALLRGTYADQQGNIYLTQEAYLSECYHVALNAKANHGKVIVQ
                     VKALVDDYQLKPNEVVIPGNLVDYVYVTEDEKNHRQVIQSHYLPALSGEERIDGIPEP
                     ALPFNSRKLILRRAAQFLTYGDTISIGYGINNELSNLLHEECVEHDVQPILDVGIFGG
                     FVGSREHFGMNYNADVRMPHDRAWDFIYNNGVSVAYLSFAEVDQYGNVNVSYFNDRLN
                     GCGGFIDITQSVNKIIFSGTFVAGSHVSCHNQRLNIETEGQNQKFVSDVSHIDFNAQY
                     SQLLEQEVYFVTDRAVFELTNQGLKLIEIAPGLDLHKDILNQMAFKPIIADHLKLIDT
                     SIYKEKWGQLKQSIHKV"
BASE COUNT         7346 a         3874 c         4008 g         7210 t
ORIGIN      
        1 atgtttctaa gtatgtgttt atgttcagta ttttggataa tttaataatt ttaaggatat
       61 taagcgctta caccgacgtg atatatttgg cttaacgaaa atgattgagg tgacagagat
      121 gaactttttt gatatccata agactccgaa caaaggcatt ccattatcgg tacaacgtaa
      181 attatggctt agaaacttca tgcaagcttt ctttgtagtg ttctttgttt atatggctat
      241 gtatttaatt cgaaacaact ttaaggcggc ccaaccgttt ttaaaagagg aaattggatt
      301 atctacatta gaacttggtt atatcggatt agcatttagt atcacgtacg gtttagggaa
      361 aacattactt ggatattttg tcgatggacg taacacaaaa cgtattatct cgttcttact
      421 tatcttatct gcgattacag ttttaattat gggatttgtt ttaagttact ttggttctgt
      481 aatgggatta ttaattgtac tttggggact taacggggtg ttccaatcag ttggtggacc
      541 tgcaagttat tcaacgattt caagatgggc gccaagaacg aaacgtggcc gatacttagg
      601 attttggaat acatcacata atatcggtgg tgccattgca ggtggtgttg cactttgggg
      661 tgctaatgta ttcttccatg gaaatgttat agggatgttc attttcccat cggtgattgc
      721 attacttatt ggtatcgcaa cattatttat cggaaaagat gatccagaag aattaggatg
      781 gaatcgtgct gaagaaattt gggaagagcc ggtcgataaa gaaaatattg attctcaagg
      841 tatgacgaaa tgggagatct ttaaaaaata tatcctggga aatcctgtta tatggattct
      901 atgtgtttca aacgtctttg tatacattgt acgaatcggt attgataact gggcaccgtt
      961 atatgtgtca gagcatttac actttagtaa aggcgatgca gttaatacga tattctactt
     1021 tgaaattggt gcattagttg caagtttatt atggggctac gtatcagact tattaaaagg
     1081 tcgtcgtgca attgtagcta ttggctgtat gtttatgatt acatttgttg tcttattcta
     1141 cacaaatgct acaagtgtca tgatggttaa catttcattg tttgcattag gtgcgttaat
     1201 ctttggtccg caattattaa ttggtgtatc attgactggt tttgttccta aaaatgccat
     1261 cagtgtagca aacggaatga caggttcatt cgcgtatcta ttcggtgact caatggcgaa
     1321 agttggtttg gcggctattg ctgatccaac acgtaacggt ttaaacatct ttggatatac
     1381 attaagtgga tggacagatg ttttcatcgt cttctatgtt gcattattcc taggcatgat
     1441 tctattagga atcgttgctt tctatgaaga aaagaaaatt agaagtttaa aaatttaata
     1501 taaatcggat taaaagtatc gccaatctat tgcaatatag ttggcaatcc tgccccgacg
     1561 gcatgtgcgt gaagagatga aagatactgc ttctaccctt gcaaatatat catctctatg
     1621 tctcggggca gatcataatt ccctgttatg aagtatcctt atttgcccga cttagggtga
     1681 ctcaatgaat ttactcctta caataaagac atatagcggt gtcaatattg tagggagtat
     1741 tgttttatat ttaaactctc taaaaagcgg actgaaagaa aagtgaaaac ttctctatca
     1801 gtccgctttt tcatagaaca aaatgtaggc gccataatca ttagttatgt gctaatctat
     1861 ttagcttgct tacaataatc acttggcgac atttgtaaat attttttaaa atgatagcta
     1921 aacattttat actctgaaaa gcctactttg tctgcaattt catagtgttt gtaatgtcga
     1981 tctaacaatt gcagagattg taaaatacga tagcgattta aataatcgac aattgtaata
     2041 ccaacatgat ctttaaatgt tcgcatcgca tacgattcac taacatcgat atgttgaatt
     2101 aaatctgaaa cagtcacttt cgtttgataa gattgcttaa tttgatcaac aatctgattt
     2161 acataataat catcgtattc tacttttaat agtggttgga aggcatcatg acaagatgct
     2221 aagctacggc cgttctgtga ttgttgctct aataaggtac ggacaagtct tcctaaaata
     2281 acttctaatt gtgcatggtc tactggtttt aataaataat caagaacatg atgttgaatg
     2341 ccggctttca tatattcaaa gtcatcgtaa ctcgataata tgatgacatt acaatctaga
     2401 agcgcaatat cattgagtaa atcgacgcca tttttacgtg gcatacgaat atcagtaatt
     2461 actaattctg gctgatgttg ttgaattagt gataatgctt caacaccatc tttagcagtg
     2521 tatattgtat tgaaatgata gtctccccaa ggaatgattt gctttaaacc ttctcgaata
     2581 attcgttcat catcacaaat aactacctta aacatctaca ttcccccttg aaagtggtat
     2641 tttataacaa attaacgtac cttgattacg ctttgaaaaa atatgaagtc gtgcatgtga
     2701 accatattga atcattgctt tattgtgtaa atgatttaat cccaaatgct tagtatcaaa
     2761 tacatcatta ttaagagatt ggcgtacata ttgcaggcga gatgacgaca tcccgatacc
     2821 attgtcgcaa actaaaacat gtaaattctg acgtgccaat gtcaggcgta tagtaatgtc
     2881 caatgactca gtatctctac catgtttaat agcattttct atgagtggct gaagcatcat
     2941 tttaccaatt gtctggtgac gcgcttcttc agaactttca atatggagct taatcatgtc
     3001 atcaaaacgg atgttttgta ttgcaacata ctgttcaatg tagttcaact cttcgtttaa
     3061 ttccactgta tgtgagtttg tacgtaatga gtaacgtaac atttgcgata attgttggac
     3121 cacagtttgt gctaatttcg gagataacgt aattaaatat tgtattgttt gcatcgtatt
     3181 gaataggaaa tgaggctgga attggcgttc tatttccttt aactgaatat cacgcaagcg
     3241 acgttctgta tgctcgatag aatggatcag ttgctcattt gattcaaata aatcgtaaat
     3301 ataattatta atttcttcta gttcactgtt gttttttaaa ggcgtatatg tacctagatg
     3361 acgatttttg gcatagtaaa ttttttgaat aatcgtttcg atatcttttg tttgtcgttt
     3421 agccatatta tctgcgctaa tgaaaccaaa tattactagt aaaacaagaa ctacggccat
     3481 aacaattaac aacgtgatac catcttcaat gttttcatgt atatctttat aaataatgag
     3541 acgatggtca gcatggttta attttacaga ttcattcata aatccgaatt gttgcggtct
     3601 atacttttca cctatagtaa aacggtcatc gttggcgtat aaaatattgt catattgatc
     3661 aacgataagt gcgaattgtc ggctatcttt cttaatttca cttaaacgtg gggtgttagc
     3721 catataaatt ttaagcatat atgtactatt attgaattta agctgatgcg ttgaaaataa
     3781 atacatattt ttagtgttta aatgttcata attattggtt ataaactgat ttggtccaga
     3841 taattcataa taaagtgttg cgggctgttg gtgtattaat tttaataatt cacgttttgt
     3901 agcggtaaca tcatgatgat ttgttaaatc gagctcttga aacgaattat tatgctgtgt
     3961 aataaatgtc tgaatctgct tttcagtgtg atgtaaagat gactgacttt catcaacatg
     4021 ttgatgaatc gtacgatgct caatccaaat atagatggca tagaagctta ctagtccaat
     4081 aataatgact aaaaatactg gaaaaatagt agacgcaaat aacgatcgtc tcaattgatg
     4141 tctataaggt ttgtatgccg tcattgaatc atctccaaaa atttatgatg tggaatgtcc
     4201 ggtaatttag atttcggtat taaaggtatg ttcttaagat tttcgataga ctgatcgctt
     4261 tgttcactaa catcctttcg aattgacttg gcatcgaact ctgcaactaa tcgttgttgt
     4321 actgagcggc ttgttaaata ttgcactaac tttttacgct taggatgagg gtgtgcattt
     4381 ttaactaaag caataccatc aacatttaac attgttcctt caattggata aacgattgat
     4441 acaggataac ctttgttttt ccatgtgcgt gcatcttgtt cgtagcttag acctgcgtaa
     4501 tatttacctt ttgcaacatc ttcaatgact ttagacgtct ttgacagttg catcgcatgg
     4561 ttttggaatt gatgcacatc acttactcga tgatgcatgc tataaatagc gcgcatatgt
     4621 tgatagcctg tcgttgttgt atttggattt gagtacgcaa ttttaccttt aagtataggt
     4681 tgtaataaat cttgataacc tcgaatctta atatctcctt gtaaatctga attcactact
     4741 ataactgttg gcattaatag aaaactagta acatatttat tgttcgagcg ataatcctct
     4801 aattgctgtg ttacagatgt atcttgatag ggaacaaaat cttctggatg atcaattgtt
     4861 tctgataaca caccacccat aaagacgtca ccacgctccg aaaaatcttc gttatgcaag
     4921 tttgaaagca gtacttgagt agatccatgt ttgatttcaa ttttgacatg ctcttgtttt
     4981 tcaaattcat ttaaaattgg acgaatcaag tttgattgat acggagaata aactgttaat
     5041 acatttttat ctgattcaga gtgacgcgta ttagcgcatg atgataaaaa aatgagaaat
     5101 aatagcaaga tataaatttt tgatttcatg atatcccatc aattctatgt atattttaat
     5161 acaataattt tagcaataaa tgacgcataa gtaatgttaa atatttagaa atgtttatag
     5221 atgacttgtt aagacgttgc aaatgttgtg atagcacaaa atttttgttt gtcaagacga
     5281 tttaccgagg ctgtaaaatc aaactgttat attttatttg tagctgttat ataaaaatcg
     5341 gcaagatatt gaacggttca aaagtgaatt tttacgtcaa taaaagtatt taatccagtc
     5401 tcttcatata taaaagtaaa tctttctaag tgttgattta acgcttatca acaatcattt
     5461 tttataaaca aatatatact cctaaattaa cttttaaagc aatgaaaata gtgaacatta
     5521 taactgttgt gtaacagaat gcaattagca tattactgtt acacaaatta gtacagtttc
     5581 tatgttttga catacatttg atgaaaattg tacataattt atgtgaaaaa aatcacaaca
     5641 aacatgctac aatgactatg aaaacgttaa catagcattt caaattcaca acattataca
     5701 gatggaggcg tttagtatgt tagaaacaaa taaaaatcat gcaacagctt ggcaaggatt
     5761 taaaaatgga agatggaaca gacacgtaga tgtaagagag tttatccaat taaactacac
     5821 tctttatgaa ggtaatgatt catttttagc aggaccaaca gaagcaactt ctaaactttg
     5881 ggaacaagta atgcagttat cgaaagaaga acgtgaacgt ggcggcatgt gggatatgga
     5941 cacgaaagta gcttcaacaa tcacatctca tgatgctggt tatttagaca aagatttaga
     6001 aacaattgta ggtgtacaaa ctgaaaagcc attcaaacgt tcaatgcaac cattcggtgg
     6061 tattcgtatg gcgaaagcag cttgtgaagc ttacggttac gaattagacg aagaaactga
     6121 aaaaatcttt acagattatc gtaaaacaca taaccaaggt gtattcgatg catattctag
     6181 agaaatgttg aactgccgta aagcaggtgt aatcactggt ttacctgatg catacggacg
     6241 tggacgtatt atcggtgact atcgtcgtgt agctttatat ggtgtagatt tcttaatgga
     6301 agaaaaaatg cacgacttca acacgatgtc tacagaaatg tcagaagatg taattcgttt
     6361 acgtgaagaa ttatcagaac aatatcgtgc attaaaagaa ttaaaagaac ttggacaaaa
     6421 atatggtttc gatttaagcc gtccagcaga aaacttcaaa gaagcagttc aatggttata
     6481 cttagcatac cttgctgcaa ttaaagaaca aaacggtgca gcaatgagtt taggtcgtac
     6541 atcaacattc ttagatatct atgctgaacg tgaccttaaa gcaggcgtta ttactgaaag
     6601 cgaagttcaa gaaattattg accacttcat catgaaatta cgtattgtta aatttgctcg
     6661 tacacctgat tacaatgaat tattctctgg agacccaact tgggtaactg aatctatcgg
     6721 tggtgtaggt attgacggac gtccacttgt tacgaaaaac tcattccgtt tcttacactc
     6781 attagataac ttaggtccag ctccagaacc aaacttaaca gtattatggt cagtacgttt
     6841 acctgacaac ttcaaaacat actgtgcaaa aatgagtatt aaaacaagtt ctatccaata
     6901 tgaaaatgat gacattatgc gcgaaagcta tggcgatgac tatggtatcg catgttgtgt
     6961 atcagcgatg acaattggta aacaaatgca attcttcggt gcacgtgcga acttagctaa
     7021 aacattactt tacgcaatca atggtggtaa agatgaaaaa tctggtgcac aagttggtcc
     7081 aaacttcgaa ggtattaaca gcgaagtatt agaatatgac gaagtattca agaaatttga
     7141 tcaaatgatg gattggctag caggtgttta cattaactca ttaaatgtta ttcactacat
     7201 gcacgataaa tacagctatg aacgtattga aatggcatta catgatacag aaattgtacg
     7261 tacaatggca acaggtatcg ctggtttatc agtagcagct gactcattat ctgcaattaa
     7321 atatgcacaa gttaaaccaa ttcgtaacga agaaggtctt gtagtagact ttgaaatcga
     7381 aggcgacttc cctaaatacg gtaacaatga cgaccgtgta gatgatatcg cagttgattt
     7441 agtagaacgc ttcatgacta aattacgtag tcataaaaca tatcgtgatt cagaacatac
     7501 aatgagtgta ttaacaatta cttcaaacgt tgtatacggt aagaaaactg gtaacacacc
     7561 agacggacgt aaagctggcg aaccatttgc tccaggtgca aacccaatgc atggccgtga
     7621 ccaaaaaggt gcattatctt cattaagttc tgtagctaag atcccttacg attgctgtaa
     7681 agatggtatt tcaaatacat tcagtatcgt accaaaatca ttaggtaaag aaccagaaga
     7741 tcaaaaccgt aacttaacta gtatgttaga tggttacgca atgcaatgtg gtcaccactt
     7801 aaatattaac gtatttaacc gtgaaacatt aatagatgca atggaacatc cagaagaata
     7861 tccacagtta acaatccgtg tatctggtta cgctgttaac ttcattaaat taacacgtga
     7921 acaacaatta gatgtaattt ctcgtacatt ccatgaaagt atgtaacaaa atttaaggtg
     7981 ggagcactat gcttaaggga cacttacatt ctgtcgaaag tttaggtact gtcgatggac
     8041 cgggattaag atatatatta tttacacaag gatgcttact tagatgcttg tattgccaca
     8101 atccagatac ttggaaaatt agtgagccat caagagaagt cacagttgat gaaatggtga
     8161 atgaaatatt accatacaaa ccatactttg atgcatcggg tggcggtgta acagtcagtg
     8221 gtggcgaacc attgttacaa atgccattct tagaaaaatt atttgcggaa ttaaaagaaa
     8281 atggtgtgca cacttgctta gacacatcgg ctggatgtgc taatgataca aaagcatttc
     8341 aaaggcattt tgaagaatta caaaaacata cagacttgat attattagat ataaaacata
     8401 ttgataatga caaacatatt agattgacag gaaagcctaa tacacacatc cttaacttcg
     8461 cgcgcaaact gtcagatatg aaacaacctg tatggattcg acatgtcctt gtgcctggtt
     8521 attctgatga taaagacgat ttaattaaac taggggaatt cattaattct cttgataacg
     8581 tcgaaaagtt tgaaattctg ccatatcatc agttaggtgt tcataagtgg aaaacattgg
     8641 gcattgcata tgaattagaa gatgtcgaag cgcccgatga tgaagctgtt aaagcagcct
     8701 accgttatgt taacttcaaa gggaaaattc ccgttgaatt ataaatacaa ttcagaccga
     8761 aaagaaagca tatgcaactt caagagtgaa ggggcatatg cttctttttc aattgagtat
     8821 tgagtattag caagacgtag taagtatatg agacaagtcc tacaatggtt gaaggaatac
     8881 gtttttgtaa gtagctatgc tgataaagaa tgtgatgcct tgttaaaggt ggggttccaa
     8941 tatcatcgaa tgggttatta attgctactt gcatatgaat atgagtcttt tcaaattttt
     9001 attgaccctg agtaatgaaa aatattaaga tgaaacttaa tattaaagca atgcggagcg
     9061 tgattatgaa gagaattagt aaagatatat gggcagtatt taaattactg tatcaaaata
     9121 aagggcgttt tagcattaat gccttactat tgcagttaat catgattttt attagtagta
     9181 catacttaat tttactattt aatatgatgt taaaagtagc tgggcaaagc caacttacga
     9241 ttaacaattg gatggaaatc ataagtcatc ctgccagtgt gatacttctt attatattca
     9301 tattaagtgt tgcctttctg atttatgtag agttttcatt gttagtttat atggtttatg
     9361 ccggctttga tcgacagatt attacattta aatccatttt taaaaatgcc tttgtaaatg
     9421 tgcgtaaact cataggtgta ccagttattt tctttgtcat ttatttaatg ttaatgatac
     9481 ccattgccaa cctaggacta agttcagtat taacaaaaaa tatttacata cctaaatttt
     9541 taacggaaga acttatgaaa acgacgaaag gtataatcat ttacggtacc tttatgattg
     9601 ctgtatttat attaaacttt aaattaatat ttaccttacc gttaacgatt ttaaaccgcc
     9661 agtcgttatt taaaaatatg agactaagtt ggcaaattac gaagcgaaat aagtttcgac
     9721 ttgttataga aatagttata ttggaactca tcattggtgc gattttaaca ttaattattt
     9781 caggagcaac atatcttgct atttgtgtag atgaagaagg agataagttt ttagtctcat
     9841 caattttatt tgttgtattg aaaagcgcat tgttcttcta ttatttattt acgaaattat
     9901 cattaatcag tgtgttagta ctgcacttaa aacaagagaa tgtattagac caaccgggct
     9961 tagaatttaa atatccaaaa tcgaaacgga agtctaggtt ctttataatt tcaatggtgc
    10021 ttgcagtgac atgttttatc ggttataaca tgtacttact ttacaataat actatcaata
    10081 caaatatctc cattattggc catcgtggtt tcgaagataa aggggttgaa aattctattc
    10141 cgtcattgaa agctgctgca aaagcgaatg tcgaatacgt tgagttagat acaattatga
    10201 cgaaagataa acaatttgtt gttagtcatg ataacaattt gaaacgttta acaggtgtta
    10261 ataaaaacat ttctgaatct aatttcaaag atgtcgtcgg tttgaaaatg cgtcaaaatg
    10321 gacatgaagc aaaacttgta tccttagacg aatttattga aacggctaaa caatcaaatg
    10381 tgaagctact agttgagtta aagccacatg gtaaagaacc agcagattat acacaacgtg
    10441 ttattgatat tttgaaaaag catggtgttg aacatcaata tcgtgtgatg tctttggatt
    10501 atgatgtgat gactaagttg aaaaaagaag cgccatatct caagtgtggt tatatcattc
    10561 cgttgcagtt tggtcatttt aaagaaacat cattagattt ctttgtcatc gaagattttt
    10621 cttattcgcc aagacttgtt aatcaagcgc acttggaaaa taaagaagtc tatacttgga
    10681 ctattaacgg cgaagaagat ttaacgaaat acttacaaac caatgttgat ggtattatca
    10741 cagatgaccc agcattagct gatcagatta aagaagaaaa gaaagacgaa acatacttcg
    10801 atcgttctat aagaattttg tttgaataat ataaacaaag acctctaaag ttatcaagat
    10861 gataccttca gaggtctttt taatgttgcc atctatggga taggcaatcg tttcattcgt
    10921 ttatattcat atgacaagta tttgtatggc aatttggcgt cacaaacact tacatgattt
    10981 attggtgaat tattaattgt tttgtgaatg caaagggtta gaaattgaat tgtaaatact
    11041 ttctaatctt tgtttcgctt tagtcatttg atccaaattt ttagtgcgta tagcggattt
    11101 tgcaatatag tgcgcagcta aaatatcgcg tttttgaaac gcatctaaat ttaggtacga
    11161 taatttattt aagtcagtgt ttgctattaa ttcatgtaat tgatctacaa gcgcttgatg
    11221 ttgatacgta tgtgatgtag tttcagattt gcttgctaat ttaataccag tcgtatcaag
    11281 gagcgccgct ttaataccag caactaaata tgttttgatt ttcatttgtg ttgtcatgct
    11341 ttgttactcc tttgatgtac attaatcaaa aaaattatac actattgtat attgcaaagc
    11401 taattaacta taacaaaaag atagttaatg ctttgtttat tctagttaat atatagttaa
    11461 tgtcttttaa tattttgttt ctttaatgta gattgggcaa ttacattttg gaggaattaa
    11521 aaaattatga aaaagcaaat aatttcgcta ggcgcattag cagttgcatc tagcttattt
    11581 acatgggata acaaagcaga tgcgatagta acaaaggatt atagtaaaga atcaagagtg
    11641 aatgagaaaa gtaaaaaggg agctactgtt tcagattatt actattggaa aataattgat
    11701 agtttagagg cacaatttac tggagcaata gacttattgg aagattataa atatggagat
    11761 cctatctata aagaagcgaa agatagattg atgacaagag tattaggaga agaccagtat
    11821 ttattaaaga aaaagattga tgaatatgag ctttataaaa agtggtataa aagttcaaat
    11881 aagaacacta atatgcttac tttccataaa tataatcttt acaatttaac aatgaatgaa
    11941 tataacgata tttttaactc tttgaaagat gcagtttatc aatttaataa agaagttaaa
    12001 gaaatagagc ataaaaatgt tgacttgaag cagtttgata aagatggaga agacaaggca
    12061 actaaagaag tttatgacct tgtttctgaa attgatacat tagttgtaac ttattatgct
    12121 gataaggatt atggggagca tgcgaaagag ttacgagcaa aactggactt aatccttgga
    12181 gatacagaca atccacataa aattacaaat gagcgtataa aaaaagaaat gatcgatgac
    12241 ttaaattcaa ttatagatga tttctttatg gagactaaac aaaatagacc gaattctata
    12301 acaaaatatg atccaacaaa acacaatttt aaagagaaga gtgaaaataa acctaatttt
    12361 gataaattag ttgaagaaac aaaaaaagca gttaaagaag cagacgaatc ttggaaaaat
    12421 aaaactgtca aaaaatacga ggaaactgta acaaaatctc ctgttgtaaa agaagagaag
    12481 aaagttgaag aacctcaatt acctaaagtt ggaaaccagc aagaggttaa aactacggct
    12541 ggtaaagctg aagaaacaac acaaccagtg gcacagccat tagtaaaaat tccacaagaa
    12601 acaatctatg gtgaaactgt aaaaggtcca gaatatccaa cgatggaaaa taaaacgtta
    12661 caaggtgaaa tcgttcaagg tcccgatttt ctaacaatgg aacaaaacag accatcttta
    12721 agcgataatt atactcaacc gacgacaccg aaccctattt tagaaggtct tgaaggtagc
    12781 tcatctaaac ttgaaataaa accacaaggt actgaatcaa cgttgaaagg tattcaagga
    12841 gaatcaagtg atattgaagt taaacctcaa gcaactgaaa caacagaagc ttctcaatat
    12901 ggtccgagac cgcaatttaa caaaacacct aagtatgtga aatatagaga tgctggtaca
    12961 ggtatccgtg aatacaacga tggaacattt ggatatgaag cgagaccaag attcaacaag
    13021 ccaagtgaaa caaatgcata caacgtaacg acaaatcaag atggcacagt atcatacgga
    13081 gctcgcccaa cacaaaacaa gccaagtgaa acaaacgcat ataacgtaac aacacatgca
    13141 aatggtcaag tatcatacgg tgctcgccca acacaaaaca agccaagcaa aacaaatgca
    13201 tacaacgtaa caacacatgc aaatggtcaa gtatcatatg gcgctcgccc gacacaaaaa
    13261 aagccaagca aaacaaatgc atataacgta acaacacatg caaatggtca agtatcatac
    13321 ggagctcgcc cgacatacaa gaagccaagc gaaacaaatg catacaacgt aacaacacat
    13381 gcaaatggtc aagtatcata tggcgctcgc ccgacacaaa aaaagccaag cgaaacaaac
    13441 gcatataacg taacaacaca tgcagatggt actgcgacat atgggcctag agtaacaaaa
    13501 taaatttata actctatcca aagacataca gtcaatacaa agaattatgt atctatacaa
    13561 cagtaatcat gcattctatg atgcttctaa ctgaattaaa gcatcgaaca atcggaagca
    13621 tatttctaaa ttatttattc attatagtct taaacataac atgacctaat atattactaa
    13681 cctattaaaa taaaccacgc acatctaagt gatatacgac aatcacagca ataataattg
    13741 ctttagaaag tcgtaccgaa ctggaactta caagtctagt tcgaacacac actgatgtga
    13801 gtggttttct ttattttaaa catgaacaat cagataagtt actagcatta gcaaatatta
    13861 ttaaatcaaa gggcttcgat tcataaaatt taaaacaatg attaaaatta gacgtgtaaa
    13921 tgttaaattc taaaacggaa ataaccacca tcccattaaa ccactttttt gttcaatcac
    13981 tatatttcac acagcttcat taataaaacg aaattgcttc aacccgcttc aacttcagcc
    14041 tacttcattc aataacctaa cgaatccgct tcatccaaaa tcaaccattc taacgcacat
    14101 actcaaatat agcagctgca cccatgccga caccaataca catcgtaacc atgccgtaac
    14161 ggctatcggg acgtctaccc atttcattaa gtaaacgcgc ggttaacatt gcccctgtag
    14221 cacctaatgg atgacctaaa gcaatagcgc caccattcac attcgtacgt gatatatcta
    14281 gacctacttc tttaatagat gcaatcgttt gagaagcaaa tgcttcgttc aattcgatca
    14341 aatcaatgtc ttcaacagat agattgctga gtgacaatac ttcaggaatc gcatatgcag
    14401 gcccaatacc cataattttc gggtcaacgc ctactgcctt ataaccaacg aatcgtgcaa
    14461 taggtgtcac gcctagttct ttcactttat ctccagacat taaaactaca aatcctgcac
    14521 catcagaaag tggggcagat gttcccgcag tcacagtgcc gtcagcttta aatactgtac
    14581 gtaatttggc taatgcctcc atcgtggtgt cagggcgtat aaattcatct tggtcaaaga
    14641 tatttgtgtg tacttttggt cctgcgtttg tatattcaac tgagtttacc cgtattggaa
    14701 taatttcatc tttgaaccga ccatcacgtt gtgcgtcata ggcacgttga tgacttctga
    14761 cagcataagc atcttgatct tcgcgtgata cgtcaaattg ggatgctaca ttttcagcag
    14821 ttaaacccat aggatatgac gcacctatat catcatattg taaggttgga ttgtttgtgg
    14881 gctcgttgcc acccattggt acggcactca tcaattcaac gccaccagct acaagtatat
    14941 ctccttgacc agccataatt tgattggctg caatcgcgat ggtttgtaat cctgatgagc
    15001 agtagcgatt cactgtttga cccggtaccg tgtcagataa tcccgcacgc aatgcaatcg
    15061 ttcgtgcaat gttttgtcct tgtaatcctt ctggaaaagc cgtaccaaca atgacatctt
    15121 caatcatatt cttattgaat tttccgtcaa tacgtttcaa tacgccttgt aatactttgg
    15181 ctgcgacatc atcaggtctt tcgtggaata atgcgccttg ctttgctttc gctgcggctg
    15241 aacgcccata agctacaatg tatgcttctt gcatggttat catcctctct taatgactat
    15301 cttttaatta cgtaatggct taccagtttt taacatatgt gcaattcttt catatgattt
    15361 tttagatttt agtaagtcaa taaagccaat tttctccaac gattgaatgt aacgttgatt
    15421 tataaatgta tttcttggta aatcaccacc cgctaaaatt gtggcgatat ttaaggcgat
    15481 atgataatca tggtcgctaa taaaatgccc ccgtctttgc gcatctaatt gtccttggat
    15541 caatgctttg aagtcttcac ctaaagcgat atattgatgt ctaggattcg gaatatagtt
    15601 tgtttctgct tcatatttcg cacgtttgag cgcaacttcg acacgttgtg ctgtattgaa
    15661 aataatcgta tctgtatcac gtaaataacc ataacgacgt gcctcaaagg catttgtaga
    15721 gactttcgca aatgcgatat tcgtcagtac ttttgtcatg gaagcttgtt tgtcatcaaa
    15781 cttatgcgat gtgcgtaata tgcgatcagc catttctgca aggccaccgc cactcggtaa
    15841 taagccaacg cctgcttcaa caagacctat atatgtttca cttgcagcga caacaatagg
    15901 tgagtaaagt acaagctcac agccaccgcc taaggcacga ccttgaacag ctgtgactac
    15961 tggtttcaaa ctatacttca aacgattaaa gctataatgt aatttatcaa ttgattgtgc
    16021 aacgacatca tctacaagac cgtcttcatg cgcctttttc attaagaaaa ggttagcacc
    16081 cacactgaaa ttattaccat ctgcataaat gaccatactt gtgtaatggt cattttccag
    16141 taaatcaatg gcatcaacta acgcatcgtt gaattcatcg gtaatgacat tatttttact
    16201 ttgtaatttc agtaacagtt gatcatcatg agttacggaa agtttggcat cacctttatc
    16261 ccaaagttca tcttttacga agtgagaaac aggtgttgca tattcaatgg tctcatcttg
    16321 tttataaaag ccaccatcta aatcactaat ccattgtggt aagtctccaa gttcgtcttc
    16381 catacgtgtt ttaacacgtt cgtatcccat tgcatcccat aattggaatg gaccaagttt
    16441 ccagttgaac ccccagacaa gcgcacggtc tatgtctcgg aaatcatcgg tagctttagg
    16501 tacattgata gcagagtaat agaaattatt acgtaatgtc tcccataaaa atagtcccgc
    16561 ttcgtcttgc gcattgaata tgacatcaag gttatgcact aagtctttat taaattcatt
    16621 taaaattggt aattgtggtt gcgatacagg tacataatct tgtttttcaa catcgtaaac
    16681 aagtcgagct ttagtttctt tatccttttt gtaaaatcct tgtttcgttt tacgtccgag
    16741 tgcgccattg tcaaacaacg tatttacaat tttgacatca tgaaaataag gcgtttcttc
    16801 aggtacttgt tgcatgcctt taattacaga cactgcaata tctaaaccga ctaggtcgga
    16861 tagggcatat gtacctgttt taggacgacc aatcgcttgc ccagttaaag catccacatc
    16921 tacaatgctt aacttgtgtt gctcggcgcg atacataata tcattcattg tttgcgtgcc
    16981 gactctattt gcgacaaagc caggcacatc attgacgaca atgacacctt tacctaatac
    17041 attttgcgcg aaatttttta catctaatat gatagattcc ttcgtgtgtg acgtaggtat
    17101 taactccact aatttcataa tacgtggtgg gttaaagaaa tgtagaccaa agaatcgttc
    17161 ttgatccttc tcgttaaatg cttgagcaat cgcattaatt ggaatacctg atgtatttgt
    17221 agcaaataaa gcatcttctt tagcatgttg tagaacttgt tgccaaacag catgcttaat
    17281 ttcaatatct tctttgactg cttcgatata taaatcagca tcatcattta ccaagtcatc
    17341 atcaaaatta ccatatgtta aatgactcac tagatttaag tcgaatagta gcggccgttt
    17401 cttatctgta attttatcgt aagatttttt cgcaatgaga tttggatcgt ttttgtccac
    17461 tacaatatct aatagtttta ctttaagtcc agcattcaca aagagtgctg ccagttgagc
    17521 gcccatcgtg cctgcgccaa gaacggttac tttattaatt gtcatagtga ttcctccaat
    17581 ttagttgagg ataagataac cattaagata attggaataa cgttgctatt ttataaaatt
    17641 aattaagtat ctttgacagt catcttagcc tcttatttaa ggaaaaagct ttatgcttaa
    17701 aataagtctt ttttagtgaa attaatgcat ctcatataat tatttgctat ttatacgaaa
    17761 gcagaatctc cagtcaaagc gcgtccaatt actaaggcat taatttcatg tgtaccttcg
    17821 tacgtgtaaa tcgcttctgc atcagagaag aaacgtgcaa tatcataatc gtcagctagt
    17881 atgccattac cacctgtaat accgcggccc atagctactg tctcacgcaa acgtaaggca
    17941 ttcatcatct tcgccgttga agttgcaacc tcgtcatatt caccatgtgc ttgcatatta
    18001 gctaattgag cacatgttgc cattgcttga gctaaattac cttgcatcat tgctagcttt
    18061 tcttgtatta actgatattt actaattggt ttgccgaatt gcttacgctc agtgacataa
    18121 tctaatgtgg cacgtaaagc gccagccata ccacctgtag ccatataagc aacgcctgct
    18181 ctcgttgaat aaagaatttt ggcaatatct ttaaagcttg ttatgttttg taagcgatcc
    18241 gcttcatcta ctttgacatt agttaattta attagtgcgt taggaacaat gcgaagtgcg
    18301 attttattat caatgacttc aatatcgacg ccatcttgtt ctggtctgac tacaaagcaa
    18361 tggggtttgc cagttttttt atttactgcg aatactggaa tgacatcaga tacatgtgca
    18421 ccaccaatcc atttcttttc accattgata acccaagtat cgccttggcg ttcagcgact
    18481 gtttcaagac ctcccgcaac gtccgaaccg tgttctggtt cagttaaagc aaagcatgta
    18541 cgcagttcat gtgactgtaa tttaggtaca tatttcgcaa tttgttcttt gctacctccg
    18601 aaatagaaag tgttatgccc taaaccttgg tgaacaccga gtagggtagc taaggaaata
    18661 tcaaatcgcg cgagtaggta agacatgaaa aactgaaata gttgactagg cattttggcg
    18721 tttggacgat ccttgtaaag taatggattg ttaaaataat ttaattctcc cagatcttta
    18781 aaatagtcct cgggtacagt agcgtctatc caatgttgat taatattttc acggtaatta
    18841 ctttctagca atgaatctac ttgttgtaaa aattcgactt caccgtctgt taaaccttta
    18901 gcaatactaa gtacatcttc aggaaataat gtttttaaga ccgtttcttt ttcaaatgtc
    18961 atataaattc ctcctaaaaa taatatgaat actaatgtga aatgcattta attcaaaaac
    19021 aacacgcttt atttgtaaac gcttacacta aatgtcaaaa atttttatca cctttaaagt
    19081 gtttgtgaga ctttgtcatt catcatttgt cgaatcgcaa gtttatctgg tttctgcgta
    19141 ctgtttaacg gcatatgtgt cactggtaca tacattcttg ggactttata acctgctaaa
    19201 cgacttcgca tatgttgatc taaaatttca gcgtaatgag gttcatcttc gcgaagtata
    19261 atggctgcag caattgattc accatatttt ggatgatcat agccaacgac cacacaccgg
    19321 tctactagtg gatgctcagc taaagcattt tcgacttcag atggtaagac attttcgcca
    19381 ccagttatga ttaattcttt tttgcggtca ataataaata tatcgccatc gtcgtccatc
    19441 ttcgctaagt caccagttaa taaataacga ccatgaaatg ctttggcagt ctctgctggt
    19501 ttattccaat atcctggcgt gacattttta gccttaattg caagttcgcc aatctcacca
    19561 gtaggtactt cctcaccgtt atcatcaagg atacgtgcat caacgaacat gactgcttta
    19621 ccaatactca ttggcttacg ttttgaattt tccggtgtat taacaagtac aagaggtgct
    19681 tcagttaaac catagccgtt aataatgttt atgccatatt gtttaaaagc tgcttggata
    19741 cttggtaatg gttgtgaacc accttggatg atataatcca tagctctaaa attttcagga
    19801 ttaaaattac tagcacgtag cgtactataa tacattgtcg gaatcatgat aataaatgta
    19861 gggtgatatt gtgcaatcat gtcattcaat tcttcgccgt taaagtaacg ttgaagaata
    19921 agtgtgccac ctgacattaa tactggtaat acagtatcgt taaaccctaa aacatggaac
    19981 attggtgttg atacaatcgt aatatagttt gaattgaact tgtacgtcag ctctaagttt
    20041 gcaccgttat gaacaaatga ttcatatgag aacatcacac ctttaggtga tccggttgta
    20101 ccacttgtat aaattaatgc tgcaagatct tgtggttcaa caggtgttgc ttgaaaaggt
    20161 tggtgataat ctggatttac gatttcatca tattgcgcca catcaatatc catatgcaat
    20221 aagttttggt caatatcggt gagtgaactt aaatgttttt cagcatagaa gagcagtttt
    20281 aattgtgcat cttccacaat ggctgcaatt tcttttgggt taagccgcca attcaatggt
    20341 aaaaaaaccg cacctgtttt aaaacaagca aacaataaat ctaatattgc aatatcattt
    20401 ggcgcaaaaa taccgataac atcgcctttt ttaacacctt gagatgttaa ataatgtgcc
    20461 atattatcag cgcgtgcatt gagttgttgg tatgtccaag atgtttgttt tgcgtgatca
    20521 ataacggcag gcttgtcatc atcgaagtct gaacgcgttt ttatccaatc gaaattcatt
    20581 agtatacccc ctttagcttc actttcatac tttatgaatt gattgtttaa gttgtcccca
    20641 tttttctttg taaatgctgg tatcaattaa ttttaaatga tcagcaataa ttggtttaaa
    20701 agccatttga ttcaaaatat ctttatgcaa atcaagacct ggtgcaattt caattagttt
    20761 caagccttga ttggtgagct cgaatactgc acgatcagta acaaaataga cttcttgctc
    20821 gagtaattgt gaatattgtg cattaaagtc gatatggctc acatctgata caaatttctg
    20881 gttttgtcct tcagtttcaa tgtttaatcg ttgattatga catgagacat gactgccagc
    20941 tacaaaagta cctgaaaaga taattttatt tacagattgc gtaatgtcta taaagccacc
    21001 acatccattt agtcggtcat tgaagtaaga cacgttgaca ttgccgtatt gatcaacctc
    21061 agcaaagcta agataggcaa ctgatacacc attgttataa ataaaatccc atgctcgatc
    21121 atgaggcatg cgcacatctg cattgtaatt cataccaaaa tgttcacgac tcccaacgaa
    21181 tccaccgaaa atgccaacat ctagaatcgg ttgcacatca tgttcaacac attcttcgtg
    21241 caataaatta gagagttcat tattgatgcc ataaccgatg ctaattgtat cgccataagt
    21301 taaaaactga gcagcacgtc ggagaatcaa tttgcgacta ttaaaaggta atgcgggttc
    21361 aggtattcca tcaattcgtt cttctccaga caaggctggt aaataatgac tctgaattac
    21421 ttggcggtga ttcttttcat cttctgtgac gtatacataa tcgacaagat ttcctgggat
    21481 aacaacttca ttcggtttta gttgatagtc gtcaactaaa gctttaactt gtacaataac
    21541 tttcccatga ttagctttcg cgtttaatgc gacatgataa cactcgctca agtacgcttc
    21601 ttgagttaaa taaatgttac cttgttgatc tgcgtatgtt cctctcagta gtgccacatc
    21661 aacgctaggg aatgtgtaat gtaagtatgt ttcatcgttg atggttacta atgaaactaa
    21721 atcatcagtt gttcgtgtat ttactttacc gccaccgtat ctaggatcaa cagctgtgtt
    21781 taatccgatt ttagtaataa ctccaggtaa taattgatta ctctgacgat aatgagttgc
    21841 aatgatacct tgtggtaaaa aataagcttc aatgtcatta tttttcattg cttgtgccgt
    21901 tttggaagaa gccgttaaaa tgctcataat gacacgttta atcatgcgac gttctataaa
    21961 atcatctaaa tccggtgcgg cacctaaact atggatatca ttcgctaata taaacgttaa
    22021 atctttgggc gtatgatatg tgtcatgttg cgctaacaca gcacgtagaa cttcggcggg
    22081 taagttggct acagctaatg ctggtaaacc aatcacatca ccatctttaa tgatatgttg
    22141 taagtcgtgc catgtgattt gtttcaagca agtcacctcc atcacatttg ataaaatata
    22201 gcgtttttac actttgtgta aacccttaca agaaatataa cataacgacg tttaaaatca
    22261 attagaaata tctttttatt ctgataatag acacagtata gacacatttt aatggttgat
    22321 aacaattgta atatcaaggg tttgtaatga attgaatatc attaaaatac ttatataaaa
    22381 atattgctcg gaatataaaa agttaaatag gttttgagtt ttaaatatga aatacaaa
//
LOCUS       DANBWH010000019        22406 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.019, whole genome shotgun sequence.
ACCESSION   DANBWH010000019 DANBWH010000000
VERSION     DANBWH010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 22406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22406
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.019"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            124..471
                     /gene="sarR"
                     /locus_tag="PPY92_000497"
     CDS             124..471
                     /gene="sarR"
                     /locus_tag="PPY92_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarR"
                     /protein_id="HDJ2624521.1"
                     /translation="MSKINDINDLVNATFQVKKFFRDTKKKFNLNYEEIYILNHILRS
                     ESNEISSKEIAKCSEFKPYYLTKALQKLKDLKLLSKKRSLQDERTVIVYVTDTQKANI
                     QKLISELEEYIKN"
     gene            complement(660..1496)
                     /locus_tag="PPY92_000498"
     CDS             complement(660..1496)
                     /locus_tag="PPY92_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HDJ2624522.1"
                     /translation="MDEQQWTGQLDLTVFFDGNRSVSRDIFFEKALKVIRPVYLNQST
                     IPTFYIVNVGGGYLDGDRYRMNVNVEDNAKVTLTSQGATKIYKTPSNHVEQYQTFNLK
                     DNAYLEYVADPIIAYENAKFYQHNTFNLNNSSSLFYTDILTPGYSKTGEAFKYQYMHL
                     INEIYIEDELVTYDNLLLNPNKQSINEIGYMEHYSHYGSAYFIHEDVNQKLIDSVYET
                     ISSYSNTFDCRVAISQLPTHGFAVRIFAYRTQIIEKILGTIQSYIAENIYDRKLDFLR
                     KY"
     gene            complement(1496..2110)
                     /gene="ureG"
                     /locus_tag="PPY92_000499"
     CDS             complement(1496..2110)
                     /gene="ureG"
                     /locus_tag="PPY92_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HDJ2624523.1"
                     /translation="MANPIKIGIGGPVGAGKTQLIEKVVKRLSKEMSIGVITNDIYTK
                     EDEKILVNSGVLPESRIIGVETGGCPHTAIREDASMNFAAIDELLERHDDIELIFIES
                     GGDNLAATFSPELVDFSIYIIDVAQGEKIPRKGGQGMIKSDFFVINKTDLAPYVGASL
                     EQMAEDTKVFRGKRPFTFTNLKTDEGLDEVIDWIERDTLLKGLS"
     gene            complement(2123..2812)
                     /locus_tag="PPY92_000500"
     CDS             complement(2123..2812)
                     /locus_tag="PPY92_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HDJ2624524.1"
                     /translation="MIDHTHLRLFQFCDSQFPTGAFSHSFGLETYIQRNIIHDDHTFI
                     AWLKMFLQEQLTYSDGLAMRLVYDALENDDTQKVLHIDKLMFVQNLPKETRVGAKQMG
                     TRMVKLALELYNSPWIAWYHQQMQDKKAKLNPAICFTMLGHHLGVDIETIIDYYLYQN
                     VSSLTQNAVRAIPLGQTAGQKIVTHMIPYIEETRKQIFELKEADFGMTAPGLELNQMA
                     HENVNVRIFIS"
     gene            complement(2805..3257)
                     /gene="ureE"
                     /locus_tag="PPY92_000501"
     CDS             complement(2805..3257)
                     /gene="ureE"
                     /locus_tag="PPY92_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HDJ2624525.1"
                     /translation="MIVEEIQGNIANLSNSEKQKHVEKVYLENSDLVKRIQRVVTDHG
                     TEIGIRLKQPIDLQYGDILYADDHNMIIVDVNSEDLLVIQPRTLQEMGDIAHQLGNRH
                     LPAQFTETEMLVQYDYLVEDLLKSLGIPYVREDRKVNKAFRHIGHSHD"
     gene            complement(3270..4985)
                     /gene="ureC"
                     /locus_tag="PPY92_000502"
     CDS             complement(3270..4985)
                     /gene="ureC"
                     /locus_tag="PPY92_000502"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HDJ2624526.1"
                     /translation="MSFKMTQNQYTSLYGPTVGDSIRLGDTNLFAQIEKDYAVYGEEA
                     TFGGGKSIRDGMAQNPRVTRDDVNVADLVISNAVIIDYDKVVKADIGIKNGYIFAIGN
                     AGNPDIMDNVDIIIGSTTDIIAAEGKIVTAGGIDTHVHFINPEQAEVALESGITTHIG
                     GGTGASEGSKATTVTPGPWHIHRMLEAAEGLPINVGFTGKGQATNPTALIEQINAGAI
                     GLKVHEDWGATPSALSHALDVADEFDVQIALHADTLNEAGFMEDTMAAVKDRVLHMYH
                     TEGAGGGHAPDLIKSAAFSNILPSSTNPTLPYTHNTVDEHLDMVMITHHLNAAIPEDI
                     AFADSRIRKETIAAEDVLQDMGVFSMISSDSQAMGRVGEVITRTWQVAHRMKEQRGPL
                     DGDFEHNDNNRIKRYIAKYTINPAITHGISEYVGSIEPGKLADIVLWDPIFFGVKPEL
                     VVKGGLINSAVNGDANGSIPTSEPMKYRKMYGQYGGNLTSTSMTFVSKTAYENGINRA
                     LNLKRMVRPVKNIRQLSKADMKNNSATPKLDVDPQTYEVYVDGEKITSNAATELPLTQ
                     RYFLF"
     gene            complement(4982..5392)
                     /locus_tag="PPY92_000503"
     CDS             complement(4982..5392)
                     /locus_tag="PPY92_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HDJ2624527.1"
                     /translation="MIPGEIITKSTEVEINNHHPETVIEVENTGDRPIQVGSHFHFYE
                     ANAALDFEREMAYGKHLDIPAGAAVRFEPGDKKEVQLVEYAGKRKIFGFRGMVNGPID
                     ESRVYRPTDENDAYAGVFGDNGAENVNKKGGKRS"
     gene            complement(5406..5708)
                     /locus_tag="PPY92_000504"
     CDS             complement(5406..5708)
                     /locus_tag="PPY92_000504"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HDJ2624528.1"
                     /translation="MHFTQREQDKLMIVVAAEVARRRKARGLKLNHPEALALISDELL
                     EGARDGKTVAELMSYGRQILNKEDVMDGVEHMITDIEIEATFPDGTKLITVHHPIV"
     gene            5968..6873
                     /gene="yut"
                     /locus_tag="PPY92_000505"
     CDS             5968..6873
                     /gene="yut"
                     /locus_tag="PPY92_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HDJ2624529.1"
                     /translation="MKIIDVLLKNISQVVLISNKWTGLFILIGLFVADWTIGLAAIVG
                     SIIAYTFARFINYSEAEVNDGLAGFNPVLTAIALTIFLDKSGLDIVITMIATLLTLPV
                     AAAVREVLRPYKVPMLTMPFVIVTCFTILLSGQVKFVDTSLKLMPQNIETVNFSNNDR
                     IHFIQSLFEGFSQVFIEASVIGGVCILIGILIASRKAALLAVIASLLSFIIVALLGGN
                     YDDINQGLFGYNFVLMAIALGYTFKTAINPYISTFLGVLLTVVVQLGTTTLLEPFGLP
                     ALTLPFIIVTWILLFAGIKHDKVDA"
     gene            7090..8244
                     /locus_tag="PPY92_000506"
     CDS             7090..8244
                     /locus_tag="PPY92_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HDJ2624530.1"
                     /translation="MNDMDNSFLITTEIQRKWIEKFKVIRDTFKAKAEYNDQHSQFPY
                     KNIEWLIKEGYGKLTLPKAYGGEGATIEDMVILQSFLGELDGATALSIGWHVSVVGQI
                     YEQKLWSQDMLEQFAVEINNGALVNRAVSEAEMGSPTRGGRPSTHAVKADDGYILNGV
                     KTYTSMSKALTHIIVAAYIEELESVGFFLVDRNLPGVEIADNWDVLGMRATESHDLIL
                     NDVKVPLKHLVETEKSKAPNGWILHIPSCYLGIAQAARNYAVDFAIQHSPNSIEGTIA
                     TLPTVQQNLGKMETLLLCARQFLWSTAKGYQQYKDDSQIRNPTSASKVMVMNQGLEVI
                     DLAMRIVGAKSLEMNRPLQRYYRDMRAGLHNPPMEDAAYTNIAKSITDTF"
     gene            8458..9366
                     /locus_tag="PPY92_000507"
     CDS             8458..9366
                     /locus_tag="PPY92_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDJ2624531.1"
                     /translation="MKKLLLPLIIMLLVLAACGNQGEKNNKAETKSYKMDDGKTVDIP
                     KDPKRIAVVAPTYAGGLKKLGANIVAVNQQVDQSKVLKDKFKGVTKIGDGDVEKVAKE
                     KPDLIIVYSTDKDIKKYQKVAPTVVVDYNKHKYLEQQEMLGKIVGKEDKVKAWKKDWE
                     ETTAKDGKEIKKAIGQDATVSLFDEFDKKLYTYGDNWGRGGEVLYQAFGLKMQPEQQK
                     LTAKAGWAEVKQEEIEKYAGDYIVSTSEGKPTPGYESTNMWKNLKATKEGHIVKVDAG
                     TYWYNDPYTLDFMRKDLKEKLIKAAK"
     gene            9764..10705
                     /locus_tag="PPY92_000508"
     CDS             9764..10705
                     /locus_tag="PPY92_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDJ2624532.1"
                     /translation="MTKKVYFNHDGGVDDLVSLFLLLQMENVQLIGVSTIGADCYLEP
                     SLSASVKIINRFSNEDIQVAPSYERGKNPFPKEWRMHAFFMDALPILNEPVKHVASNV
                     SDKEAFEDIIQTLKRQSEKVTLLFTGPLTDLAKALQKDSSIVQYIEKLVWMGGTFLPK
                     GNVEEPEHDGSAEWNAYWDPEAVKIVFDSDIEIDMVALESTNQVPLTLDVRQRWANER
                     QYTGIDFLGVSYAAVPPLTHFITNSTYFLWDVLTTAYIGNKDLVHSIEKKVDVISYGP
                     SQGKTFECKDGRKINVINHVDNNAFFDYITALAKKVN"
     gene            10826..11380
                     /locus_tag="PPY92_000509"
     CDS             10826..11380
                     /locus_tag="PPY92_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDJ2624533.1"
                     /translation="MTTKQLVYTALMTAIIAILGMVPVIPLPFSSVPIVLQNIGIFLA
                     GVILGRKYGTLSVIVFLLLVVAGLPLLSGGRGGIGVFAGPSAGFLLLYPVVAFMIGAI
                     RDRFINEINFWILFVGILVFGVIALDVIGTLIMGMIINIPFTKAISISLAYLPGDILK
                     AIVASLIGTALLNHSQFRQIMGLK"
     gene            11457..12227
                     /locus_tag="PPY92_000510"
     CDS             11457..12227
                     /locus_tag="PPY92_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDJ2624534.1"
                     /translation="MASISMSDIYCNGTIFENDDEQLIYLTPSFPQRYTSNTWIYKKT
                     PTQERWLKDLERQHQLHTNQGSNHYAFSFPENEQLDNHWMAMFKDMNFELGIMELYAI
                     ESDALANLPRNSDVEIAVVDESHIDAYLKVAYQFSLPFGKDYADAHEEMVREHYQKDV
                     IKRLVAYLNNEPIGVVDVIESENYIELDGFGVLEQFRHQGIGSTIQSLIGEYAISKNH
                     KPIILVTDGEDTAKDMYAKQGYVYQSFCYQILKEDIGN"
     gene            complement(12399..13196)
                     /gene="fdhD"
                     /locus_tag="PPY92_000511"
     CDS             complement(12399..13196)
                     /gene="fdhD"
                     /locus_tag="PPY92_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDJ2624535.1"
                     /translation="MNKDVSLGQPIVRYEDGKLFNTTDQYVTEFPLTIMVNGEEFATV
                     ICSPTNLEELVIGFLASEGAILKRDELKSVLIDDSKGFAHVELNKDLGDRFQYSTKRM
                     IASCCGKSREFYFQNDAAIAKTSMSKITLTPIQIINMMTRLQSASHIYQETGGLHNAA
                     ISDGLTFFVHRQDIGRHNALDKLYGFCIQRHITVRDKVLIFSGRISSEILIKAAKIGV
                     GVILSKSAPTTLAVTLANDLNITAVGFIRNGGFNIYSHPERIIDSEQ"
     gene            13485..14267
                     /gene="modA"
                     /locus_tag="PPY92_000512"
     CDS             13485..14267
                     /gene="modA"
                     /locus_tag="PPY92_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDJ2624536.1"
                     /translation="MKMKRFIAIVMALFLVLAGCSNSNDNNESKKDDADNGKKQEIQV
                     AAAASLTDVTKKLASEFKKEHKNADIKFNYGGSGALRKQIESGAPVDVFMSANTKDVD
                     ALKDKNKAHDTYKYAKNSLVLIGDKDSNYTSVKDLKDNDKLALGEVKTVPAGKYAKQY
                     LDNNNLFKEVESKIVYAKDVKQVLNYVEKGNAKQGFVYKTDLYKQNKKIDTVKVIKEV
                     ELKKPITYEAGATSDSKLAKEWMEFLKSDKAKEILKEYHFAA"
     gene            14281..14952
                     /gene="modB"
                     /locus_tag="PPY92_000513"
     CDS             14281..14952
                     /gene="modB"
                     /locus_tag="PPY92_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDJ2624537.1"
                     /translation="MPDLTPFWISIRVAVISTIIVTVLGIFISKWLYRRKGSWVKVLE
                     SLLILPIVLPPTVLGFILLIIFSPRGPIGQFFANVLHLPVVFTLTGAVIASVIVSFPL
                     MYQHTVQGFRGIDTKMINTARTMGASETKIFLKLILPLAKRSILAGIMMSFARALGEF
                     GATLMVAGYIPNKTNTLPLEIYFLVEQGRENEAWLWVLVLVAFSIVVISTINLLNKDK
                     YKEVD"
     gene            14953..15558
                     /locus_tag="PPY92_000514"
     CDS             14953..15558
                     /locus_tag="PPY92_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDJ2624538.1"
                     /translation="MLKINVKYQLKNTLIRINIDDTEPKIYAVRGPSGIGKTTVLNMI
                     AGLRKADEAIIEVNGQLLTDTTKNVNVKIQQRRIGYLFQDYQLFPNMTVYKNITFMAE
                     PSKHIDQLIQTLNINHLMKQYPMTLSGGEAQRVALARALSTKPDLILLDEPFSSLDDT
                     TKDESITLVKRIFNEWQIPIIFVTHSNYEAEQMAHEIITIG"
     gene            15728..16732
                     /locus_tag="PPY92_000515"
     CDS             15728..16732
                     /locus_tag="PPY92_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HDJ2624539.1"
                     /translation="MSQERYSRQILFKQIGEIGQSKINQKCALIIGMGALGTHVAEGL
                     VRAGIAKLIIVDRDYIEFSNLQRQTLFTEEDALKMMPKVVAAKKHLLALRSDVDIDGC
                     IAHVDYYFLETHGQDVDVIIDATDNFETRQLINDFAYKHRIPWIYGGVVQSTYTEAAF
                     IPGKTPCFNCLVPQLPALNLTCDTVGVIQPAVTMATSLQLRDAMKVLTEQPIDTKITY
                     GDIWEGSHYSFGFSKMQRSDCTTCGDVPSYPYLNKNEQRYATLCGRDTVQYENASITH
                     DILVQFLKQHQLNYRSNSYMVMFEFKGHRIVAFKGGRFLIHGMTRTSDATHLMNLLFG
                     "
     gene            16763..17269
                     /locus_tag="PPY92_000516"
     CDS             16763..17269
                     /locus_tag="PPY92_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaB"
                     /protein_id="HDJ2624540.1"
                     /translation="MGEHQNVKLNRTVKAAVLTVSDTRDFVTDKGGQCVRQLLQADDV
                     EVSDAHYTIVKDEKVAITTQVKKWLEEDIDVIITTGGTGIAQRDVTIEAVKPLLTKEI
                     EGFGELFRYLSYVEDVGTRALLSRAVAGTVNNKLIFSIPGSTGAVKLALEKLIKPELN
                     HLIHELTK"
     gene            complement(17271..17765)
                     /gene="moaC"
                     /locus_tag="PPY92_000517"
     CDS             complement(17271..17765)
                     /gene="moaC"
                     /locus_tag="PPY92_000517"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDJ2624541.1"
                     /translation="MTEFTHINQQGHAKMVDVSDKQITKRTAVAHSSITVNETIFKQI
                     SNNTNTKGNVLNTAQIAGIMAAKNTSTIIPMCHPLPLTGIDVHFSWDETNAPLYTLNI
                     QTTVSTTGKTGVEMEALTAASATALTIYDMTKAVDKGMIIGETYLESKSGGKSGDFQR
                     QSGQ"
     gene            17828..19087
                     /locus_tag="PPY92_000518"
     CDS             17828..19087
                     /locus_tag="PPY92_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDJ2624542.1"
                     /translation="MVVEKRNPIPVKEAIQRIVNQQSSMPAITVALEKSLNHILAEDI
                     VATYDIPRFDKSPYDGFAIRSVDSQGASGQNRIEFKVIDHIGAGSVSDKLVGDHEAVR
                     IMTGAQIPNGADAVVMFEQTIELEDTFTIRKPFSKNENISLKGEETKTGDVVLKKGQV
                     INPGAIAVLATYGYAEVKVIKQPSVAVIATGSELLDVNDVLEDGKIRNSNGPMIRALA
                     EKLGLEVGIYKTQKDDLDSGIQVVKEAMEKHDIVITTGGVSVGDFDYLPEIYKAVKAE
                     VLFNKVAMRPGSVTTVAFADGKYLFGLSGNPSACFTGFELFVKPAVKHMCGALEVFPQ
                     IIKATLMEDFTKANPFTRFIRAKATLTSAGATVVPSGFNKSGAVVAIAHANCMVMLPG
                     GSRGFKAGHTVDIILTESDAAEEELLL"
     gene            19084..19569
                     /gene="mobB"
                     /locus_tag="PPY92_000519"
     CDS             19084..19569
                     /gene="mobB"
                     /locus_tag="PPY92_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDJ2624543.1"
                     /translation="MILQIVGYKKSGKTTLMRHIVSFLKTHGYTVATIKHHGHAKEDI
                     QLQDSDVDHMKHFEAGADQSIVQGFQYQQTVTRVDNQNLTQIIEKSVTIDTNIVLVEG
                     FKNADFEKVVVYRDEEELQVLQQLSNVCYSINVREHEDFTAFEQWLLNKIKNDCDTQL
                     T"
     gene            19583..20029
                     /locus_tag="PPY92_000520"
     CDS             19583..20029
                     /locus_tag="PPY92_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HDJ2624544.1"
                     /translation="MKQFEIVIEPIQTEQYREFTINEYQGAVVVFTGHVREWTKGVKT
                     EYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSIVHRIGPLQISDIAVLIAVSSPHRK
                     DAYRANEYAIERIKEIVPIWKKEIWEDGSKWQGHQKGNYEEAKREE"
     gene            20035..20268
                     /gene="moaD"
                     /locus_tag="PPY92_000521"
     CDS             20035..20268
                     /gene="moaD"
                     /locus_tag="PPY92_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HDJ2624545.1"
                     /translation="MKVLYFAEIKDILQKAQEDIVLEQALTVQQFEDLLFERYPQINN
                     KKFQVAVNEEFVQKSDFIQPNDTVALIPPVSGG"
     gene            20275..20874
                     /gene="mobA"
                     /locus_tag="PPY92_000522"
     CDS             20275..20874
                     /gene="mobA"
                     /locus_tag="PPY92_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HDJ2624546.1"
                     /translation="MKAIILAGGHSVRFGKPKAFAEVNGETFYSRVIKTLESTNMFNE
                     IIISTNAQLATQFKYPNVVIDDENHNDKGPLAGIYTIMKQHPEEELFFVVSVDTPMIT
                     GKAVSTLYQFLVSHLIENHLDVAAFKEDGRFIPTIAFYSPNALGAITKALHSDNYSFK
                     NIYHELSTDYLDVRDVDAPSYWYKNINYQHDLDALIQKL"
     gene            20894..21916
                     /gene="moaA"
                     /locus_tag="PPY92_000523"
     CDS             20894..21916
                     /gene="moaA"
                     /locus_tag="PPY92_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDJ2624547.1"
                     /translation="MVEQIKDKLGRPIRDLRLSVTDRCNFRCDYCMPKEVFGDDFVFL
                     PKNELLTFDEMARIAKVYAELGVKKIRITGGEPLMRRDLDVLIAKLNQIDGIEDIGLT
                     TNGLLLKKHGQKLYDAGLRRINVSLDAIDDTLFQSINNRNIKATTILEQIDYATSIGL
                     NVKVNVVIQKGINDDQIIPMLEYFKDKHIEIRFIEFMDVGNDNGWDFSKVVTKDEMLT
                     MIEQHFEIDPVEPKYFGEVAKYYRHKDNGVQFGLITSVSQSFCSTCTRARLSSDGKFY
                     GCLFATVDGFNVKAFIRSGVTDEELKEQFKALWQIRDDRYSDERTAQTVANRQRKKIN
                     MNYIGG"
     gene            complement(22182..22367)
                     /locus_tag="PPY92_000524"
     CDS             complement(22182..22367)
                     /locus_tag="PPY92_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624548.1"
                     /translation="MFRFISFGQLVVYSTTFIPKKDILQLNDLECDINVKEPLKINYV
                     VIFGSGTEITFSQNLFR"
BASE COUNT         7709 a         3328 c         4155 g         7214 t
ORIGIN      
        1 attgaatttt tatacttatt tgtttagaat gaactttata acatagttgg atagagtttc
       61 gatttaataa attacatgtg aaccttgcta caacaagatg tgcatcagag gagtggttta
      121 ataatgagta aaattaatga cattaatgat ttagtcaacg caacatttca agttaagaag
      181 tttttcagag atacaaaaaa gaagttcaat ttgaactatg aagaaattta tattttaaat
      241 catattttaa gaagtgagtc taacgaaatc tcatctaaag agattgctaa gtgctcagag
      301 ttcaaacctt actatttaac taaagcttta caaaagctaa aagatttaaa attgttatca
      361 aagaaaagaa gtttacaaga cgaaagaaca gttattgttt atgttacaga tacacaaaaa
      421 gcaaatattc aaaaactgat ttcagaatta gaagaataca ttaaaaatta aatcaaggtt
      481 aattgcgttt aataacattg aacgataaca atttattaaa acgaagttat ttattcagca
      541 ttgggacata aaattaactt aaaatttaaa tattgaagat gctttaatta aagttaaaga
      601 ctagccatac cttatttcag cttattaagc ttgaaacaag gtacactagt ctttttattt
      661 taatattttc ttagaaaatc aagtttacga tcataaatat tttctgcgat atagctttgg
      721 atggttccaa gtattttctc tataatttgt gtgcgataag caaaaattct aactgcaaag
      781 ccatgtgtag gcaattgaga aatagcaaca cgacaatcga atgtattgct ataagaacta
      841 atggtttcat aaactgaatc gattagtttt tggttaacat cttcatgtat gaagtaagca
      901 gagccataat gagagtaatg ttccatataa cctatttcat tgatcgattg tttattagga
      961 ttcaataata aattatcata tgtgactaac tcatcttcaa tataaatttc atttattaaa
     1021 tgcatatatt gatacttgaa ggcttcgcca gtttttgaat aaccaggagt taaaatatca
     1081 gtataaaata atgaactaga attattgaga ttgaacgtat tgtgttgata aaatttagca
     1141 ttttcatatg cgatgattgg atcagcgaca tattctaaat atgcgttatc ttttaaatta
     1201 aaagtttgat actgctcaac atgattggaa ggtgtcttgt atatttttgt tgcaccttga
     1261 gatgtcaatg tcactttagc gttatcttcg acattcacat tcatacggta acgatctcca
     1321 tctaaatagc caccacctac atttactata taaaatgtag gaatggtaga ttgatttaga
     1381 taaactggac gtatcacttt aagtgctttt tcaaagaaaa tatctcttga tactgatcga
     1441 ttgccatcga aaaacactgt taaatcaagt tgcccagtcc attgttgttc atccattatg
     1501 ataatccttt gagtaaagtg tcgcgttcaa tccaatcgat aacttcatcc aaaccttcgt
     1561 cggtttttaa gttagtaaaa gtaaatggac gtttaccacg aaatacttta gtatcttcag
     1621 ccatttgttc taatgatgca cctacatagg gagctaaatc agttttgtta attacaaaga
     1681 aatctgactt aatcatacct tgaccacctt tacgtggaat cttttcacct tgagcaacat
     1741 cgataatata tattgaaaag tcaacaagtt ctgggctaaa tgttgctgct aagttatcgc
     1801 caccagattc tatgaaaatt agttctatat cgtcatgacg ttctaataat tcgtctattg
     1861 ctgcaaagtt catagatgca tcttcacgaa tcgcagtatg aggacatcca ccagtttcaa
     1921 caccaatgat acgactttca ggtagaactc ctgaatttac taatatcttt tcgtcttctt
     1981 ttgtatatat atcatttgta ataacgccga tactcatttc ttttgaaaga cgttttacaa
     2041 ctttttcaat taattgtgtt ttacctgcac ctacaggacc accaatacca attttaatcg
     2101 gatttgccac aattataacc tcctatgata tgaaaattct aacattgacg ttctcatgcg
     2161 ccatttgatt tagttctaaa ccaggcgctg tcatgccaaa atctgcttct tttaattcga
     2221 aaatctgctt tcttgtttct tctatataag gaatcatgtg agtaactatc ttttgaccag
     2281 cagtttgtcc aagtggaata gcacgaacag cattttgagt taaacttgaa acattttgat
     2341 ataaatagta atcaataatc gtttcaatat ctacacctaa atgatggcct agcatagtaa
     2401 aacaaatagc tggatttaac tttgctttct tatcttgcat ttgttgatga taccaagcaa
     2461 tccatgggct attataaagt tctaaagcca atttaaccat gcgagtcccc atttgttttg
     2521 caccaacacg tgtttcttta ggtaagtttt gaacaaacat cagtttatct atgtgtaata
     2581 ctttttgtgt atcatcattt tccaatgcat cataaactaa acgcatggct aaaccatcag
     2641 aataggtaag ttgctcttgt aaaaacattt ttaaccaagc aataaaagta tgatcgtcat
     2701 gaattatatt tcgttgaata tatgtttcaa gaccaaatga atgactgaaa gcacctgttg
     2761 gaaactgtga atcacagaac tgaaataatc ttaagtgtgt atgatcaatc atgagaatgc
     2821 cctatatgtc tgaaagcctt attaacttta cggtcttctc gaacatatgg gatgcctaaa
     2881 ctttttaata aatcttcaac taaataatca tattgtacta gcatttcagt ctctgtaaat
     2941 tgtgctggca aatgacgatt tcctaattga tgggctatat ctcccatttc ttgcaatgtt
     3001 cttggttgaa tcactaaaag atcttctgaa ttaacatcca caataatcat attatggtca
     3061 tctgcgtata aaatatctcc atattgtaag tcaataggtt gttttaaacg aatgcctatt
     3121 tcagtgccat ggtctgtaac gactctttga atacgtttaa caagatctga attttcaagg
     3181 tatacttttt cgacgtgctt ttgtttttct gaatttgaca aattggcaat attgccttgg
     3241 atttcttcaa caatcattct atgttcctcc tagaataaga agtatctttg agttaatggt
     3301 aactcagttg ctgcattact tgtaattttt tctccatcta catatacttc atatgtttgt
     3361 ggatcaacgt ctaatttagg tgttgcactg ttatttttca tatctgcttt agataattgt
     3421 ctaatatttt taactggacg caccatgcgt tttaaattta atgcacgatt gataccattt
     3481 tcataagcag ttttagacac gaatgtcatt gacgtacttg taaggtttcc gccgtattga
     3541 ccatacattt tacggtactt catcggttca gatgtaggta tagaaccatt tgcatcgcca
     3601 tttacggcag agttaattaa tccgcccttt acaactaatt caggtttaac cccaaagaaa
     3661 attgggtccc ataagacaat gtcagctagt ttgcccggct cgatagatcc tacatattca
     3721 gaaataccat gtgtaattgc tgggttaatt gtatatttag cgatataacg tttgatgcga
     3781 ttattatcat tatgttcaaa atcaccatct aaaggaccac gttgttcttt catgcgatgt
     3841 gctacttgcc atgttcgtgt aattacttca cctacacggc ccattgcttg tgaatcggaa
     3901 ctaatcatac tgaatacacc catatcttgc agaacatctt ctgctgcaat cgtttcttta
     3961 cgaatacgtg aatctgcgaa tgcgatatct tcaggaatag ccgcatttaa atggtgagta
     4021 atcattacca tatctaaatg ttcatctaca gtattatgtg tataaggcaa agttggattt
     4081 gtagatgaag gtaaaatatt tgaaaatgca gcggatttaa ttaaatcagg cgcatgaccg
     4141 ccaccagcac cttcagtatg gtacatatga agtacacggt ctttaacagc agccattgtg
     4201 tcttccataa atcctgcttc atttaaagta tctgcatgta atgcaatttg aacatcaaat
     4261 tcatcagcaa catctaatgc atgactcaaa gcagatggtg ttgcacccca gtcttcatgt
     4321 acttttaatc caattgctcc ggcattgatt tgttcaatga gtgcagttgg atttgttgct
     4381 tgtcctttac ctgtaaaacc gacattaatc ggtaaacctt cggcagcttc taacattcta
     4441 tgaatatgcc atggacctgg agttacagtt gttgctttag aaccttctga agcaccagta
     4501 ccaccaccaa tatgagtcgt aataccactt tctaatgcga cctctgcttg ttcaggatta
     4561 ataaaatgaa catgagtatc aataccacca gcagtgacga ttttaccttc agcggcaatg
     4621 atatctgttg ttgaacctat aataatgtcg acattatcca ttatatctgg gttgccggca
     4681 ttacctatgg cgaaaatata accattttta atgcctatat cagctttaac cactttatcg
     4741 taatcgataa taacggcatt agaaatgaca aggtctgcaa cgttcacgtc atcacgtgtt
     4801 acacgaggat tttgcgccat accgtctcta atagatttac caccaccaaa agtagcttct
     4861 tcaccataaa ccgcatagtc tttttctatt tgagcaaata gattcgtatc acctaaacga
     4921 atagaatctc caacagttgg accgtataag ctcgtatatt gattttgcgt cattttaaag
     4981 ctcatgatct ttttcctcct tttttattca cgttttcagc accgttatct ccgaatacac
     5041 ctgcatatgc atcattttca tcagttgggc gatagacacg tgactcatcg ataggaccat
     5101 tgaccatacc acgaaaacca aaaattttac gtttgccagc atattcaact aattgaactt
     5161 cttttttatc cccaggttca aatcgaacag ctgctccagc tggaatatct aaatgttttc
     5221 catatgccat ttcacgttcg aaatctaatg ctgcatttgc ttcataaaaa tgaaaatgtg
     5281 agcccacttg aattggtcga tctcctgtat tttcaacttc gataactgtt tcaggatgat
     5341 ggttattaat ttcaacctct gtactttttg taataatttc tcctggtatc atttgactgc
     5401 ctcctttaaa caatagggtg atgtactgtg attaacttag taccatcggg gaacgtagcc
     5461 tcgatttcga tatctgtaat catgtgttcg acaccatcca tgacatcttc tttgtttaga
     5521 atttgtctac cataactcat taactctgca acggtcttac catcgcgtgc accttctaat
     5581 aattcatcgc tgattaaagc taatgcctca ggatgattta gtttcaaacc acgtgcttta
     5641 cgacgacgtg caacttccgc cgccactaca atcattaatt tgtcttgctc tcgttgtgta
     5701 aaatgcaaat taaaaccccc aatttcatat tagatacaat ttacaaaatt tatattaatc
     5761 ctaattgttg tgataaacaa gtaatataca aagttcaatg tgtaattaga aaattatatt
     5821 tttagcatat ccgctattga agcaaacaat ctaatcgaaa acaaatagcg gaatatattt
     5881 atgtaaaaac caaaatagtt tttaatataa cttttcatag aatagtagta tattaatgag
     5941 taatgattca aaggaaaggt gaaagatttg aagataatag atgtgctttt gaaaaatata
     6001 tctcaggttg tgttaattag taataaatgg acaggattat ttatcttaat aggattattt
     6061 gtagccgatt ggacaattgg attagcggct attgtaggta gcatcatcgc ctatactttt
     6121 gcgcgtttta taaattatag tgaggcagag gttaatgatg ggttagccgg atttaatcca
     6181 gtgttaactg ccattgcgtt aacaatcttt ttagataagt caggattaga tattgttata
     6241 acaatgatag caactttatt aacgttacca gttgctgctg cagtgagaga agttttaaga
     6301 ccatataaag ttccgatgct gacgatgcct tttgtcattg tgacttgctt tacaatttta
     6361 ctttcaggac aggttaaatt tgtagataca tcgttaaagt taatgcctca aaacattgaa
     6421 acggttaatt ttagcaacaa tgatagaata catttcattc agtcattatt tgaaggattc
     6481 agtcaagtat ttatcgaagc gagtgtaatt ggtggcgtat gtattttaat cggcatattg
     6541 atagcatcaa gaaaagcagc actcttagct gttatagcta gtttgttaag ctttatcatt
     6601 gtagctctat taggtggtaa ttatgatgat attaatcagg gattattcgg ttataacttt
     6661 gtattaatgg caatcgcact aggatataca tttaaaacag cgattaaccc ttatatttcg
     6721 acttttttag gtgtgttatt aacagtagtg gtgcaactag gtacaacaac attgcttgaa
     6781 ccgtttggct tacctgcatt aacattgcca tttattatcg tgacatggat tttattattt
     6841 gctggtatta aacatgacaa agtagatgct tgatagttaa atcaaaccta atattgtttg
     6901 aatatcacct taaaccatac agcgaattgt atagtttaag gtgtattttt atggataaaa
     6961 ttaagtgcat acttaatcga attatcagtt ttcttttaat tgttcacagc acgttccttt
     7021 ttatttttaa agtcgagtgt taaactagga ataaataatt tatattgtgt gttgtgtggg
     7081 gtgactaata tgaatgatat ggataattcc tttttaataa caacggaaat tcaaagaaaa
     7141 tggattgaaa aattcaaagt aattagagat acatttaagg ctaaagctga atataatgat
     7201 caacatagcc aatttccata taaaaatatt gaatggttaa ttaaagaagg ttatggaaaa
     7261 ttaacgttac caaaagcata tggtggtgaa ggtgcgacca tagaagacat ggttattttg
     7321 caatcatttt taggcgaact tgatggtgcc acagcattat ctattggttg gcatgtgagt
     7381 gtcgtaggac aaatttatga acagaaatta tggtctcaag atatgttgga gcaatttgct
     7441 gttgaaatta ataatggtgc attagttaat agagcagtta gtgaagctga aatgggtagt
     7501 ccaacaagag ggggaagacc aagtacacat gctgttaaag ctgatgatgg gtatatttta
     7561 aatggtgtga agacatatac atcaatgagt aaagcactaa cacatattat tgttgctgct
     7621 tatatagaag aattagaaag tgttggtttt ttcttagtag acaggaattt gcctggtgtt
     7681 gaaattgccg ataattggga cgtattaggt atgcgagcga cagaaagtca tgatttaata
     7741 ttaaatgatg taaaagtacc attaaaacat ttagtggaaa cagagaaaag taaagcgcca
     7801 aatggttgga tattgcatat accaagctgt tatttaggta tagcacaagc tgctagaaat
     7861 tatgctgtag actttgcaat ccaacatagc cctaatagta ttgaaggaac gattgcaaca
     7921 ttgccaactg tgcaacaaaa tttagggaaa atggaaacgc tattgttatg tgcgagacaa
     7981 tttttatgga gtacagcaaa agggtatcaa caatataaag atgacagtca gataagaaat
     8041 ccaacaagtg cgagtaaagt gatggtaatg aatcaagggc ttgaagtgat tgatttagcg
     8101 atgagaatag ttggtgctaa aagcttagaa atgaatcgac cattacaacg ttactatcgt
     8161 gacatgcgtg caggattaca caatccacct atggaagatg cagcatatac aaatattgct
     8221 aagagtatta cagatacatt ttaaatttaa aaaggtaaat cgatcgttga ttggcataaa
     8281 gagatgaaat ttgacgttcg gatttaccct tttttgtggg gatttatagg gatgttaaga
     8341 tattttgcta aattttttgc gaaaataaaa aaataaatag cactagctat tgtaaatgta
     8401 tatgcaatct tgtataattt tggaataatt gataatcatt ttcaatagga ggaaattatg
     8461 aaaaaactat tattaccatt aataattatg ttattagtgt tagctgcgtg tgggaaccaa
     8521 ggtgaaaaaa ataacaaagc tgaaactaaa tcttataaaa tggacgatgg caaaacggta
     8581 gatattccga aagaccctaa acgcattgca gtagttgcgc caacatatgc tggtggactt
     8641 aaaaaattag gtgcaaacat tgtagctgta aatcaacaag tcgatcaaag caaagtatta
     8701 aaagataaat ttaaaggtgt tacaaaaatt ggtgatggcg atgtagaaaa agttgctaaa
     8761 gaaaagccag atttaattat tgtatactct actgacaaag atattaaaaa atatcaaaaa
     8821 gtagcaccaa cagtagttgt tgactataat aagcataaat atttagaaca acaagaaatg
     8881 ttagggaaaa ttgttggtaa agaagataaa gtaaaagctt ggaagaaaga ttgggaagaa
     8941 acaactgcta aagacggtaa agaaattaaa aaagcaattg gacaagatgc aacagtgtca
     9001 ttgtttgatg aatttgataa aaaattatac acttacggcg ataactgggg tcgtggtgga
     9061 gaagtattat atcaagcatt tggtttgaaa atgcaaccag aacaacaaaa gttaactgca
     9121 aaagcaggtt gggctgaagt gaaacaagaa gaaattgaaa aatatgctgg tgattacatt
     9181 gtgagtacaa gtgaaggtaa acctacacca ggatatgaat caacaaacat gtggaagaat
     9241 ttgaaagcta ctaaagaagg acatattgtt aaagttgatg ctggtacata ctggtacaac
     9301 gatccttata cattagattt catgcgtaaa gatttaaaag aaaaattaat taaagctgca
     9361 aaataattca gctatataag ttagtgaaat gagagtctga aacatatcat cttttgatat
     9421 tgtattaggc tcttattttt atagctagaa agttagatat ttgtattttt ttaaataata
     9481 agtgccgttg ttatcgttca atttaattaa tgatagatta gtattattat agctaaagta
     9541 gtatacctga gaaaatagct caatgtatct ctttattgat aagttatatc ataattatat
     9601 tagtgcatac tttatggaag ggatatcagg gaatggctat caataaaaga agaggtttaa
     9661 aaggattaca acagaatgtt atgattttgt agaaagatat ataacaacgt tttataaaaa
     9721 cataatattg ttaatggaca atgaaatgta agggggattt cgagtgacta agaaagttta
     9781 ttttaaccat gatggtggtg tagatgattt agtatctcta tttttattat tacaaatgga
     9841 aaacgttcaa ttgatagggg tcagtacaat tggtgctgat tgttatttag agccatcttt
     9901 gagcgcatca gtaaaaatta ttaatcgttt ttcaaatgaa gatattcaag ttgcgccatc
     9961 atatgaacga ggaaaaaatc catttcctaa agaatggcgt atgcatgcct tttttatgga
    10021 cgcattgcca attttaaatg agccagtcaa acatgttgct tcaaatgtga gcgacaaaga
    10081 agcctttgaa gacattattc aaactttaaa gagacaatca gaaaaagtaa cattattatt
    10141 tacaggcccg cttacagatt tagcaaaagc actacaaaaa gattcatcta tcgttcagta
    10201 tatagaaaaa ttagtttgga tgggtggcac ctttttacca aaaggaaatg ttgaagaacc
    10261 tgagcatgat ggttctgcag aatggaatgc atattgggat ccagaagcgg ttaaaattgt
    10321 ttttgatagc gatatagaga ttgatatggt tgctttagaa agtacgaatc aagtgccgct
    10381 aacgttagat gttagacaaa gatgggcgaa tgaacgccaa tatacgggca ttgatttttt
    10441 aggagtaagt tatgcagcgg taccaccatt aacacacttt ataacaaatt ctacttactt
    10501 tttatgggat gttttaacga ctgcttatat tggtaacaag gacttggttc attcaattga
    10561 gaaaaaagtc gatgtaataa gttatggacc aagtcaaggt aagacatttg agtgtaaaga
    10621 tgggcgcaaa attaatgtca taaatcatgt agataacaac gcattttttg attatataac
    10681 tgcacttgct aaaaaagtaa attaacagct gtgtagaata attaaggttt taatttatat
    10741 agaacaactt attgtaaact tttcatttct taaagtttac aatggtgcta taataatggt
    10801 catgaaatac gaaaggaagt aaaaaatgac aacaaaacag ttagtatata cagctttaat
    10861 gacagcgatt atcgctattt taggaatggt accggtaatt ccactaccat tttcttcagt
    10921 accaattgta cttcaaaaca ttggtatttt cttagcaggt gtgattttag gacgtaaata
    10981 tggcacatta agtgttatcg tctttttatt attagtagtt gctggcttgc cattgttatc
    11041 aggtggtcgc ggtggcatcg gtgtattcgc aggtccttca gcagggtttt tactattata
    11101 tccagttgta gcattcatga ttggtgcgat tcgtgataga ttcatcaatg aaattaattt
    11161 ctggatttta ttcgttggta ttttagtttt tggtgttata gcattagatg ttattggtac
    11221 attgattatg ggcatgatta ttaacatacc atttacgaaa gctatttcaa tttcattagc
    11281 ttatttgcct ggtgatatat taaaagcaat tgtagcaagt ttgattggta cagctttact
    11341 taatcactcg cagtttcgtc aaattatggg attaaaataa tcatatttaa gttaagataa
    11401 taaagtaatt gaataagttg ctttgaaatt tataaaagtg aaaggagtag gtgtcaatgg
    11461 ctagtataag tatgtcagat atatattgta acggcactat atttgaaaat gacgacgagc
    11521 agttaattta tttaacgcct tcttttccac aacgatacac aagcaacaca tggatatata
    11581 aaaagacgcc tacccaagag cgatggctga aagacttaga acgtcaacat caattacata
    11641 caaatcaagg ttcaaatcat tatgcgttta gtttcccgga aaatgaacaa cttgataatc
    11701 attggatggc tatgtttaaa gatatgaatt ttgaactagg tattatggaa ttgtatgcca
    11761 tagaaagtga tgcgcttgct aatttgccgc gtaactctga cgttgaaatt gccgtcgttg
    11821 acgagtcgca tatagatgcc tatttaaaag ttgcatatca gtttagtctg ccatttggaa
    11881 aagactatgc agatgcacat gaagaaatgg taagggaaca ttatcaaaaa gatgtgatta
    11941 aacgcttagt agcttattta aataatgaac ctattggcgt tgtagatgtc attgaaagtg
    12001 aaaattacat tgaattagat ggatttggtg tattagaaca atttcgacac caaggaattg
    12061 gatctacaat tcaatcgttg ataggtgaat acgccatatc aaaaaatcac aaaccaatca
    12121 tattagttac agatggtgaa gatacagcaa aagatatgta tgcaaagcaa ggttatgtct
    12181 atcaatcgtt ttgttatcaa atattaaaag aagatattgg aaattaacga ttgatgatag
    12241 gtttgtatta gaataaaaaa gcagtaagct ggtttcgagt agaaatcaac ttactgcttt
    12301 ttaaattgtt ttgagctact tatacttata aaaatagtgc gtttaaattg ttgattcatg
    12361 tagaatatcg ttcattatga cacactataa tgaatatgtt attgttcaga gtcgatgata
    12421 cgttctggat gactatatat attaaagcca ccatttcgaa taaaacctac tgccgtaata
    12481 tttaggtcat tagctaaggt tacagcaagc gttgtcggag ctgatttaga taaaatgacg
    12541 ccaacaccga ttttcgcggc tttaattaaa atttctgatg aaatacgtcc actaaaaatt
    12601 aatactttat ctcggacagt aatatgtcgc tggatacaaa atccatataa tttatctaga
    12661 gcgttatgtc taccaatgtc ttgtcgatgt acaaaaaatg tcaaaccatc gcttatagca
    12721 gcattatgta agccacctgt ttcttggtaa atatgacttg cactttgtaa tcgagtcatc
    12781 atgttaataa tttgtattgg agttaaagtg attttagaca tagaagtttt agcgatagca
    12841 gcatcatttt gaaaataaaa ctcacgactc tttccacaac aagatgcaat cattcgtttt
    12901 gtggaatatt gaaagcgatc gcctaaatct ttattaagtt caacatgggc aaaaccttta
    12961 ctatcatcaa tcagcacaga ttttaattca tctcgcttta aaatggcacc ttccgaagcc
    13021 agaaatccaa tgactaactc ctcaaggttt gttggactgc atataacagt cgcaaattct
    13081 tcaccattca ccataattgt aagtggaaat tctgtcacat attgatctgt tgtattgaat
    13141 aattttccat cttcatatct aacaattggt tgacctaaag atacatcttt gttcattatc
    13201 taaccccttt aattagctta aactttattt taaagcaatt tgcttaaaat tttaacatat
    13261 ttgcttaagt ttgaaatttg atttataaaa ttaatagcga gcaatctgtt tgatttaaat
    13321 tgaattcgag agtatacata ctaggggatc aattaataaa tatcaatctt atgcaaattt
    13381 gacaattgtt tgaatcaata tataaacagg caacggttct tttcaaatat aatagtaagt
    13441 gtataatgaa aatgtaaata ttattaaaaa tgggggttca ctcaatgaaa atgaaacgtt
    13501 ttatagctat tgtaatggca ttatttttag tattagctgg ttgctctaat tctaacgata
    13561 ataatgaaag taaaaaagat gacgcagaca atggtaagaa acaagagatt caagttgcag
    13621 cggcagcaag tttaacagat gtaaccaaga aattagcttc agaatttaaa aaagagcata
    13681 aaaatgctga tattaaattt aactatggtg gatcaggggc attaagaaaa caaattgaat
    13741 caggcgcacc tgttgacgta tttatgtctg caaatactaa agatgtagat gcattaaaag
    13801 acaagaataa agcgcatgat acatataaat atgcgaaaaa tagtctagta ttaattggtg
    13861 ataaagattc aaattacact tcagtaaaag acttaaaaga caatgataaa ttagcattag
    13921 gtgaagtgaa aactgtacca gcaggaaaat atgcgaaaca gtatttagat aacaataact
    13981 tatttaaaga agtcgaaagt aaaatcgttt atgctaaaga tgtaaaacaa gtattaaatt
    14041 atgttgaaaa gggtaatgcg aaacaaggtt ttgtgtataa aactgactta tataaacaaa
    14101 acaaaaaaat tgatactgta aaagtaatta aagaagtaga acttaagaaa ccaatcacat
    14161 acgaagctgg tgctacatca gatagtaaat tagcaaaaga gtggatggaa ttcttaaaat
    14221 cagataaagc taaagaaata ttaaaagaat accactttgc agcataagga gttgtaatcc
    14281 atgcctgact taacaccttt ttggatatca atacgagttg ctgtaatcag tacgattatt
    14341 gtaacggttt taggtatttt tatatctaaa tggttgtatc gtcgtaaggg ttcgtgggtt
    14401 aaagtattgg aaagtttatt gatattacct attgttttgc cgccaacggt attaggtttt
    14461 attctattaa tcatcttctc gccaagagga ccaatcggtc aattctttgc gaatgtacta
    14521 catttacctg tagtgttcac tttgacaggt gctgtgatag catctgtcat tgttagtttt
    14581 ccactaatgt atcaacatac tgtgcaaggc ttcagaggta tagacacgaa aatgattaat
    14641 acagctagaa cgatgggagc aagtgaaacg aaaattttcc tcaaattaat tttaccatta
    14701 gctaaacgct ctattttagc aggtataatg atgagttttg ctcgtgcatt aggtgagttt
    14761 ggtgctacat taatggttgc aggatatatt ccaaataaaa cgaatacact acctttagaa
    14821 atatacttct tagtggaaca aggtagagaa aatgaagcgt ggttatgggt attagtgcta
    14881 gtcgcattct ctattgtggt tatatctaca attaatttat tgaataaaga taaatataag
    14941 gaggtcgact agatgcttaa aatcaatgtg aaatatcaat taaagaacac tttaattcgc
    15001 atcaatatag atgatactga accaaaaatt tatgcagttc gtggtccatc tggcattggt
    15061 aaaactactg ttttaaatat gattgccgga ttacgtaaag cagatgaagc tattatcgaa
    15121 gtgaatgggc aattgcttac tgatacgaca aaaaacgtga atgttaaaat tcaacaacga
    15181 cgtattggat atctgtttca agactaccaa ttgtttccta atatgacggt ttataaaaat
    15241 attactttta tggctgaacc atctaaacac atcgatcaat taattcaaac tttaaacatt
    15301 aatcatttga tgaaacaata tcctatgaca ttgtcaggtg gagaggcaca acgtgtagca
    15361 cttgcacgtg cacttagcac gaaaccagat ttaattttat tagatgaacc tttttctagt
    15421 ttggatgata ctacaaaaga tgagagtatt acattagtta aacgtatttt caacgaatgg
    15481 caaataccaa tcatatttgt gacacattca aactatgaag cagaacaaat ggctcatgaa
    15541 attattacaa ttgggtaatc atttatttgc cattaaagag tttagaacgt atttaaaatt
    15601 gtagaagtga atgcttctat cagcatttta atgatgtttt aaactctttt ttaggggcag
    15661 tttttttgag agacattgac gcgcgtcata taatgaaagt aatgataaaa agaaaggata
    15721 acttaatgtg agtcaagaac gttattcaag gcaaatttta tttaaacaaa taggtgaaat
    15781 aggtcaaagc aaaataaatc aaaaatgtgc gttgattatt ggtatgggcg cattaggtac
    15841 acatgtggcc gaaggacttg ttagagcagg cattgctaaa ctaatcattg ttgatagaga
    15901 ttatattgaa tttagtaatt tacaaagaca aacattgttt actgaagaag atgctttgaa
    15961 aatgatgcct aaagtggttg cagctaaaaa gcatttgcta gcgttacgta gtgatgttga
    16021 tattgatggt tgtattgccc atgtggatta ttattttttg gaaacacatg gacaggacgt
    16081 tgacgttatt attgatgcaa ccgataactt tgaaacacga caactgatta atgattttgc
    16141 atataaacat cgtatacctt ggatttatgg tggtgttgta cagagtacat atacagaagc
    16201 tgcatttata cctggtaaaa caccttgctt taactgtttg gtaccacaat tgccagcatt
    16261 aaatttaaca tgtgatacag taggggtcat tcaacctgcc gtgacgatgg caacaagttt
    16321 acaattaaga gatgcgatga aagtattaac ggaacaacca attgacacaa aaataactta
    16381 tggcgatatt tgggaaggta gtcattattc atttggtttc agtaaaatgc aacgttcaga
    16441 ctgtacaact tgtggagatg taccaagtta tccgtattta aacaagaatg aacaacgtta
    16501 tgcaacattg tgtggtagag acactgtaca gtatgaaaat gcatcaatta cacacgacat
    16561 tcttgttcaa tttttaaaac aacatcaact aaattatcgc agtaattcgt atatggttat
    16621 gtttgaattt aaaggacacc gcattgttgc ttttaaaggt ggaaggtttt taatacatgg
    16681 catgacacgc acatcagatg ccacacatct aatgaattta ttgtttggat aaaaaaagat
    16741 aagacaaaag gagtgtaata ttatgggcga acatcaaaac gttaaattga atcgtacagt
    16801 taaagcagcc gtactaacgg tatcagatac tagagacttt gttacagata aaggtggtca
    16861 atgcgtgcgc caactattac aagcagatga cgttgaagtg agtgacgcac attatacaat
    16921 tgtgaaagat gaaaaagtag ccatcacgac gcaggtgaag aagtggttag aagaagatat
    16981 tgatgtcatc attacgactg gtggaacagg tattgcacaa cgtgatgtga cgattgaagc
    17041 agtaaaacca cttttaacta aagagataga aggctttggg gaattgttta gatatttgag
    17101 ttatgttgaa gatgttggca cgcgtgcatt attgtctcgt gctgtagcag gtacagttaa
    17161 taataaattg atattttcga ttccaggatc aacaggcgca gttaaattag cattagaaaa
    17221 gctcattaaa ccagaattaa atcatctgat tcatgagctt acaaaataat ttattgacct
    17281 gattggcgtt gaaaatctcc agatttaccg ccagacttgc tttcaaggta ggtttcgcca
    17341 ataatcatac ctttatcaac tgctttcgtc atgtcgtaaa tggttaaagc cgttgctgat
    17401 gcagcggtta aagcttccat ttcaacaccg gttttgccag ttgtagagac agttgtttga
    17461 atgtttaaag tataaagggg tgcatttgtt tcatcccagc tgaagtgaac atctatgcca
    17521 gtcaatggta atggatggca catcggaata attgttgatg tatttttggc agccataata
    17581 ccagcgattt gagcagtgtt caatacattg cctttggtat tggtgttatt agaaatctgc
    17641 ttaaaaatag tttcattcac agtaatgctt gaatgagcga cagcagttct ttttgtaatt
    17701 tgtttgtctg atacatcgac cattttggcg tggccttgtt gattaatatg agtaaactca
    17761 gtcattttac ccctcctagt gcatctagta tatcatgaaa aaataaaagt tttggagatg
    17821 atttttaatg gtagtagaaa aaagaaaccc aatcccagtt aaagaagcaa ttcaacgtat
    17881 cgttaatcag cagagttcaa tgccggcaat tacggtagca cttgaaaaaa gtctaaatca
    17941 tatcttagca gaagatattg tagctactta tgatatacca aggtttgata aatcacctta
    18001 tgatggtttt gcaattcgca gtgttgattc acaaggggca agtggtcaga atcgcattga
    18061 gtttaaagtg attgatcata ttggtgcagg ttcagtttct gataaattag ttggggatca
    18121 cgaagcggtg cgtattatga ctggagcaca aataccaaat ggcgcagatg ctgttgttat
    18181 gtttgaacaa acgattgaac tagaagatac atttacaatt cgtaaaccat tttcaaaaaa
    18241 tgaaaatata tctttaaaag gtgaagaaac aaagacaggc gatgttgttc taaaaaaagg
    18301 acaagtaatt aacccagggg ctatcgcggt ccttgcaaca tatggctatg cagaggttaa
    18361 agttattaag caaccgagtg tcgctgttat tgcaacagga agcgaattat tagatgttaa
    18421 tgatgtatta gaagatggaa aaattcgtaa ctctaatggc ccaatgattc gtgccttagc
    18481 agaaaaatta ggtcttgaag ttggtattta caaaacacaa aaagatgatt tagatagtgg
    18541 catccaagtc gttaaagaag ctatggaaaa acacgatatc gttattacaa cgggtggagt
    18601 ttctgttgga gactttgact atttacctga gatttataag gctgtaaagg cggaagtgtt
    18661 atttaataaa gtagcaatgc gtcctggtag cgtaacaacg gttgcatttg cagatggaaa
    18721 gtatttgttt ggattatctg gaaatccatc agcttgtttt acaggatttg aactatttgt
    18781 gaaaccagct gttaaacata tgtgtggcgc actagaagtc ttcccgcaaa taattaaagc
    18841 aacattaatg gaagatttta ccaaggcaaa cccattcaca cgatttatac gtgctaaagc
    18901 aacgttaaca agtgctggag ctactgtagt accttcagga ttcaataaat caggtgcggt
    18961 tgtagcgatt gcacatgcta actgtatggt catgttacca ggagggtcac gtggttttaa
    19021 agcggggcat acagtagata ttatattgac tgaatctgac gctgctgaag aggaacttct
    19081 tttatgattt tacaaattgt aggttacaaa aagtctggta agacaacatt gatgaggcat
    19141 attgtctctt tcttaaagac acatggttat acagttgcta ctattaaaca tcatgggcat
    19201 gctaaggaag atattcaatt acaggattca gacgtcgatc acatgaagca ttttgaagcg
    19261 ggggcagatc aaagtattgt acaaggtttt caatatcagc aaactgtaac acgtgtagat
    19321 aatcaaaatc ttactcaaat tattgagaaa tctgttacaa ttgacaccaa tatcgtatta
    19381 gttgaaggct ttaaaaatgc tgattttgaa aaagttgtag tctatcgaga tgaagaagag
    19441 ttgcaagtat tacaacaatt gtcgaatgtt tgttatagca ttaatgtaag ggagcatgaa
    19501 gattttacag catttgagca atggttatta aataaaatta aaaatgattg tgatacacaa
    19561 ttaacataga ggattgaaat gaatgaaaca atttgaaatc gtgatagaac cgatacaaac
    19621 agaacaatat cgtgaattca ctataaatga atatcaaggt gcagtagttg tttttaccgg
    19681 tcatgttcgc gaatggacta aaggcgtcaa aacggaatat ttagaatatg aagcgtatat
    19741 tccaatggct gaaaagaaat tggcacaaat tggagatgaa ataaatgaaa aatggcctgg
    19801 aacgataacg agtattgttc atagaatagg gccattacaa atttcagata tcgctgtatt
    19861 aattgcagtt tcttcaccgc atcgtaaaga tgcctatcga gcaaatgaat atgcaattga
    19921 gcgtataaaa gaaattgttc cgatttggaa aaaagaaatt tgggaagatg gttcaaaatg
    19981 gcaagggcat caaaaaggga attatgaaga agcgaagagg gaggaataag agagatgaag
    20041 gtactttact tcgcagaaat taaagatata ttacaaaaag cacaggaaga tattgtgctt
    20101 gaacaagcat tgactgtaca acaatttgaa gatttattgt ttgaacgtta tccgcaaatc
    20161 aataataaaa agtttcaagt tgctgtaaat gaggaatttg tacaaaaatc agatttcatt
    20221 caacctaatg atactgttgc attaattcca ccggttagtg gaggttaagg gagcatgaaa
    20281 gcaataattc ttgcaggtgg tcattcagtg cgatttggta agcccaaagc ttttgcggaa
    20341 gtgaacggtg agacctttta tagtagagta attaagacat tagaatcaac aaatatgttc
    20401 aatgaaatta ttattagtac aaatgcgcaa ttggcaacgc aatttaaata tccaaatgtt
    20461 gttatagatg atgagaatca taatgataaa ggtccattag caggaattta tacaatcatg
    20521 aagcaacatc ctgaagaaga attgtttttt gtcgtttctg ttgatacacc aatgattact
    20581 ggtaaagctg taagcacgtt gtatcagttt ttagtttctc atcttattga aaatcattta
    20641 gatgtcgcag cttttaaaga agatggacgt tttattccaa caattgcatt ttatagtccg
    20701 aatgcattag gcgctataac taaagcacta cattctgata attacagttt taaaaatata
    20761 tatcatgaat tatcaacgga ttatttggat gtcagggatg tagatgcgcc ctcatattgg
    20821 tacaaaaata taaattatca gcatgatttg gacgctttaa ttcaaaaatt gtaagctgtt
    20881 aggaggtcca caaatggtag aacaaataaa agataaacta ggacgtccca tccgtgactt
    20941 acggttatct gtgacagatc ggtgtaactt taggtgtgat tattgcatgc ctaaagaggt
    21001 atttggagat gatttcgtat ttttacctaa aaatgaactg ttaacgtttg atgaaatggc
    21061 tagaatcgct aaggtatatg cagaattagg tgtaaaaaaa atacgcatta caggtggaga
    21121 accattgatg cgacgcgatt tagatgtact tatagctaaa ttaaatcaaa tcgatggtat
    21181 tgaagatatt ggtttgacta caaatggttt gttattaaaa aagcatggac aaaagttata
    21241 tgatgctggg ctacgcagaa ttaatgtcag tttggatgct attgatgata cgctatttca
    21301 atcaatcaat aatcgtaata ttaaagcgac tacgatttta gaacaaattg attacgcgac
    21361 gtctattggt ttgaatgtaa aagtaaatgt tgttatacaa aaaggtatta acgatgatca
    21421 aatcatacca atgcttgaat attttaaaga taaacatata gagattcgat ttatagaatt
    21481 tatggatgtt ggtaatgata atggatggga tttcagtaaa gttgtaacta aagatgaaat
    21541 gcttacaatg atagagcagc actttgaaat cgatcctgta gaaccaaaat attttgggga
    21601 agtagcaaaa tattatcgcc ataaggataa tggtgttcaa tttggtttga ttacaagtgt
    21661 ttcacaatca ttttgttcta catgtacacg cgcaaggctg tcatcagatg ggaagtttta
    21721 cggatgttta tttgcaactg tcgatggatt taacgttaaa gcgtttattc gttctggcgt
    21781 gaccgacgaa gaattaaaag aacaatttaa agctttatgg caaataagag atgatcgata
    21841 ttcagatgag agaactgctc aaacagttgc caatcgtcaa cgtaaaaaga taaacatgaa
    21901 ttatattggt ggttaatgtg tagggaccac tacatattaa atcattagag atgttttaat
    21961 atttctgtct tactccctaa aatacaatat tatttattaa agtaaaaacg gtcatatcta
    22021 tgccagattt aatagaaatg atcgttttta aagtttttac aagttggcgg ggccccaaca
    22081 cagaagctga cagaaagtca gcttacaata atgtgcaagt tggcggggcc ccaacataga
    22141 gaatttcaaa aagaaattct acagacaatg caagttgggg atcaacgaaa taaattttgt
    22201 gaaaatgtta tttctgtccc actcccaaaa atcactacat aattaatctt taagggctct
    22261 ttaacattga tgtcacactc caagtcattg agttgtaata tatctttttt aggtataaat
    22321 gttgtcgaat aaacaacaag ttgtccaaaa gatataaatc taaacaagat atagccagca
    22381 atttaatatt tgtaatagat aaaatg
//
LOCUS       DANBWH010000020        20907 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.020, whole genome shotgun sequence.
ACCESSION   DANBWH010000020 DANBWH010000000
VERSION     DANBWH010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20907
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.020"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(48..1124)
                     /locus_tag="PPY92_000525"
     CDS             complement(48..1124)
                     /locus_tag="PPY92_000525"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDJ2624549.1"
                     /translation="MSQAVKVERRETLKQKPNTSQLGFGKYFTDYMLSYDYDADKGWH
                     DLKIVPYGPIEISPAAQGVHYGQSVFEGLKAYKRDGEVALFRPEENFKRLNNSLARLE
                     MPQVDEAELLEGLKQLVDIERDWIPEGEGQSLYIRPFVFATEGALGVGASHQYKLLII
                     LSPSGAYYGGETLKPTKIYVEDEYVRAVRGGVGFAKVAGNYAASLLAQTNANKLGYDQ
                     VLWLDGVEQKYIEEVGSMNIFFVENGKVITPELNGSILPGITRKSIIELAKNLGYEVE
                     ERRVSIDELFESYDKGELTEVFGSGTAAVISPVGTLRYEDREIVINNNETGEITQKLY
                     DVYTGIQNGTLEDKNGWRVVVPKY"
     gene            complement(1460..2425)
                     /locus_tag="PPY92_000526"
     CDS             complement(1460..2425)
                     /locus_tag="PPY92_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDJ2624550.1"
                     /translation="MKKIMITGALGQIGTELVVKCREIYGTDNVLATDIREPEADSPV
                     QNGPFEILDVTDRDRMFELVRDFEADSLMHMAALLSATAEKNPILAWDLNMGGLMNAL
                     EAARTYNLHFFTPSSIGAFGDSTPKVNTPQVTIQQPTTMYGVNKVAGELLCQYYFKRF
                     GVDTRSVRFPGLISHVKEPGGGTTDYAVEIYFKAVREGHYTSFIDKGTYMDMMYMDDA
                     IEAIIKLMEADDAKLETRNGYNLSAMSFDPEMVKEAIQEYYPNFTLDYDVDPIRQGIA
                     NSWPDSIDTSCSRGEWGFDPKYDLASMTKLMLEAIEQKDTVKNNN"
     gene            complement(2639..4276)
                     /locus_tag="PPY92_000527"
     CDS             complement(2639..4276)
                     /locus_tag="PPY92_000527"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HDJ2624551.1"
                     /translation="MSYSIGIDYGTASGRVFLINTTNGQVVSKFVKPYTHGVIESELN
                     GLKIPHTYALQNSNDYLEIMEEGISYIVRESKIDPVNIVGIGIDFTSSTIIFTDENLN
                     PVHNLKQFKNNPHAYVKLWKHHGAYKEAEKLYQTAIENNNKWLGHYGYNVSSEWMIPK
                     IMEVMNRAPEIMEKTAYIMEAGDWIVNKLTNKNVRSNCGLGFKAFWEEETGFHYDLFD
                     KIDPKLSKVIQDKVSAPVVNIGEVVGKLDDKMAQKLGLSKETMVSPFIIDAHASLLGI
                     GSEKDKEMTMVMGTSTCHLMLNEKQHQVPGISGSVKGAIIPELFAYEAGQSAVGDLFE
                     YVAKQAPKSYVDEAANRNMTVFELMNEKIKHQMPGESGLIALDWHNGNRSVLSDSNLT
                     GCIFGLTLQTKHEDIYRAYLEATAFGTKMIMQQYQDWHMEVEKVFACGGIPKKNAVMM
                     DIYANVLNKKLIVMDSEYAPAIGAAILGAVSGGAHNSINDAVDAMKEPILYEINPEAE
                     KVQRYETLFKAYKALHDIHGYKKANIMKDIQSLRVEG"
     gene            complement(4435..5313)
                     /gene="hchA"
                     /locus_tag="PPY92_000528"
     CDS             complement(4435..5313)
                     /gene="hchA"
                     /locus_tag="PPY92_000528"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HDJ2624552.1"
                     /translation="MSQDVNELSKQPTPDKAEDNAFFPSPYSLSQYTAPKTDFDGVEH
                     KGAYKDGKWKVLMIAAEERYVLLENGKMFSTGNHPVEMLLPLHHLMEAGFDVDVATLS
                     GYPVKLELWAMPTEDEAVISTYNKLKEKLKQPKKLADVIKNELGPDSDYLSVFIPGGH
                     AAVVGISESEDVQQTLDWALDNDRFIVTLCHGPAALLSAGLNREKSPLEGYSVCVFPD
                     SLDEGANIEIGYLPGRLKWLVADLLTKQGLKVVNDDMTGRTLKDRKLLTGDSPLASNE
                     LGKLAVNEMLNAIQNK"
     gene            complement(5583..6770)
                     /locus_tag="PPY92_000529"
     CDS             complement(5583..6770)
                     /locus_tag="PPY92_000529"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDJ2624553.1"
                     /translation="MVQSLHEFLEENINYLKENGLYNEIDTIEGANGPEIKINGKSYI
                     NLSSNNYLGLATNEDLKSAAKAAIDTHGVGAGAVRTINGTLDLHDELEETLAKFKGTE
                     AAIAYQSGFNCNMAAISAVMNKNDAILSDELNHASIIDGCRLSKAKIIRVNHSDMDDL
                     RAKAKEAVESGQYNKVMYITDGVFSMDGDVAKLPEIVEIAEEFGLLTYVDDAHGSGVM
                     GKGAGTVKHFGLQDKIDFQIGTLSKAIGVVGGYVAGTKELIDWLKAQSRPFLFSTSLA
                     PGDTKAITEAVKKLMDSTELHDKLWDNAQYLKNGLSKLGYDTGESETPITPVIIGDEK
                     TTQEFSKRLKDEGVYVKSIVFPTVPRGTGRVRNMPTAAHTKDMLDEAIAAYEKVGKEM
                     KLI"
     gene            6941..8116
                     /locus_tag="PPY92_000530"
     CDS             6941..8116
                     /locus_tag="PPY92_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDJ2624554.1"
                     /translation="MLDWFQLANNKENKTIQLRRYLHQYPELSFEEFQTHDYIVNQLS
                     QLSCDIETPIGRNGIKATFKGLGTGPTIALRADFDALPVEELNDVPYKSKNPGCMHAC
                     GHDGHTAILLTVAEILDEHKHLLEGNVVLIFQYGEEIMPGGSQEMIDAGCLENVDRIY
                     GTHLWSGYPTGTIHSRAGAIMASPDEFSVTIKGRGGHGAKPHETIDPIVIMAEFILSA
                     QKIISRTIDPVKQAVLSFGMIQAGTTDSVIPDQAFCKGTVRTFDSDIQNHVMDKMDKL
                     LQGLAIANDINYDLNYIKGYLPVHNNEKAYQVIKEATNDLHVRFNESDLMMIGEDFSH
                     YLKVRPGAFFLTGCGNESKGITAPHHNPKFDIDEKSLKYAVAVFLKIIELEQVFKTN"
     gene            complement(8398..9582)
                     /gene="tuf"
                     /locus_tag="PPY92_000531"
     CDS             complement(8398..9582)
                     /gene="tuf"
                     /locus_tag="PPY92_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDJ2624555.1"
                     /translation="MAKEKFDRSKEHANIGTIGHVDHGKTTLTAAIATVLAKNGDSVA
                     QSYDMIDNAPEEKERGITINTSHIEYQTDKRHYAHVDCPGHADYVKNMITGAAQMDGG
                     ILVVSAADGPMPQTREHILLSRNVGVPALVVFLNKVDMVDDEELLELVEMEVRDLLSE
                     YDFPGDDVPVIAGSALKALEGDAQYEEKILELMEAVDTYIPTPERDSDKPFMMPVEDV
                     FSITGRGTVATGRVERGQIKVGEEVEIIGLHDTSKTTVTGVEMFRKLLDYAEAGDNIG
                     ALLRGVAREDVQRGQVLAAPGSITPHTEFKAEVYVLSKDEGGRHTPFFSNYRPQFYFR
                     TTDVTGVVHLPEGTEMVMPGDNVEMTVELIAPIAIEDGTRFSIREGGRTVGSGVVTEI
                     IK"
     gene            complement(9799..11880)
                     /gene="fusA"
                     /locus_tag="PPY92_000532"
     CDS             complement(9799..11880)
                     /gene="fusA"
                     /locus_tag="PPY92_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDJ2624556.1"
                     /translation="MAREFSLEKTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETH
                     EGASQMDWMEQEQDRGITITSAATTAAWEGHRVNIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFVNKMDKLGANFEYSVSTLHDRLQANAA
                     PIQLPIGAEDEFEAIIDLVEMKCFKYTNDLGTEIEEIEIPEDHLDRAEEARASLIEAV
                     AETSDELMEKYLGDEEISVSELKEAIRQATTNVEFYPVLCGTAFKNKGVQLMLDAVID
                     YLPSPLDVKPIIGHRASNPEEEVIAKADDSAEFAALAFKVMTDPYVGKLTFFRVYSGT
                     MTSGSYVKNSTKGKRERVGRLLQMHANSRQEIDTVYSGDIAAAVGLKDTGTGDTLCGE
                     KNDIILESMEFPEPVIHLSVEPKSKADQDKMTQALVKLQEEDPTFHAHTDEETGQVII
                     GGMGELHLDILVDRMKKEFNVECNVGAPMVSYRETFKSSAQVQGKFSRQSGGRGQYGD
                     VHIEFTPNETGAGFEFENAIVGGVVPREYIPSVEAGLKDAMENGVLAGYPLIDVKAKL
                     YDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVILEPMMKVTIEMPEEYMGDIMGDVT
                     SRRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRSNTQGRGTYTMYFDHYAEVPKSI
                     AEDIIKKNKGE"
     gene            complement(12003..12473)
                     /gene="rpsG"
                     /locus_tag="PPY92_000533"
     CDS             complement(12003..12473)
                     /gene="rpsG"
                     /locus_tag="PPY92_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDJ2624557.1"
                     /translation="MPRKGSVPKRDVLPDPIHNSKLVTKLINKIMLDGKRGTAQRILY
                     SAFDLVEQRSGRDALEVFEEAINNIMPVLEVKARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNYARLRGEKTMEDRLANEILDAANNTGGAVKKREDTHKMAEANKAFAHYRW"
     gene            complement(12539..12952)
                     /gene="rpsL"
                     /locus_tag="PPY92_000534"
     CDS             complement(12539..12952)
                     /gene="rpsL"
                     /locus_tag="PPY92_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDJ2624558.1"
                     /translation="MPTINQLVRKPRQSKIKKSDSPALNKGFNSKKKKFTDLNSPQKR
                     GVCTRVGTMTPKKPNSALRKYARVRLSNNIEINAYIPGIGHNLQEHSVVLVRGGRVKD
                     LPGVRYHIVRGALDTSGVDGRRQGRSLYGTKKPKN"
     gene            complement(13050..13304)
                     /locus_tag="PPY92_000535"
     CDS             complement(13050..13304)
                     /locus_tag="PPY92_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HDJ2624559.1"
                     /translation="MSKEKVARFNKQHFVVGLKETLKALKKDQVTSLIIAEDVEVYLM
                     TRVLSQINQKNIPVSFFKSKHALGKHVGINVNATIVALIK"
     gene            complement(13441..17037)
                     /gene="rpoC"
                     /locus_tag="PPY92_000536"
     CDS             complement(13441..17037)
                     /gene="rpoC"
                     /locus_tag="PPY92_000536"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDJ2624560.1"
                     /translation="MKIGLASPEKIRSWSFGEVKKPETINYRTLKPEKDGLFCERIFG
                     PTKDWECSCGKYKRVRYKGMVCDRCGVEVTKSKVRRERMGHIELAAPVSHIWYFKGIP
                     SRMGLLLDMSPRALEEVIYFASYVVVDPGPTGLEKKTLLSEAEFRDYYDKYPGQFVAK
                     MGAEGIKDLLEEIDLDEELKLLRDELESATGQRLTRAIKRLEVVESFRNSGNKPSWMI
                     LDVLPIIPPEIRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPGIIVQNEK
                     RMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGKRVDYSGRSV
                     IAVGPSLKMYQCGLPKEMALELFKPFVMKELVQREIATNIKNAKSKIERMDDEVWDVL
                     EEVIREHPVLLNRAPTLHRLGIQAFEPTLVEGRAIRLHPLVTTAYNADFDGDQMAVHV
                     PLSKEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTLERKDAVNTGAIFN
                     NTNEVLKAYANGFVHLHTRIGVHASSFNNPTFTEEQNKKILATSVGKIIFNEIIPDSF
                     AYINEPTQENLERKTPNRYFIDPTTLGEGGLKEYFENEELIEPFNKKFLGNIIAEVFN
                     RFSITDTSMMLDRMKDLGFKFSSKAGITVGVADIVVLPDKQQILDEHEKLVDRITKQF
                     NRGLITEEERYNAVVEIWTDAKDQIQGELMQSLDKTNPIFMMSDSGARGNASNFTQLA
                     GMRGLMAAPSGKIIELPITSSFREGLTVLEYFISTHGARKGLADTALKTADSGYLTRR
                     LVDVAQDVIVREEDCGTDRGLLVSDIKEGTEMIEPFIERIEGRYSKETIRHPETDEII
                     IRPDELITPEIAKKITDAGIEQMYIRSAFTCNARHGVCEKCYGKNLATGEKVEVGEAV
                     GTIAAQSIGEPGTQLTMRTFHTGGVAGSDITQGLPRIQEIFEARNPKGQAVITEIEGV
                     VEDIKLAKDRQQEIVVKGANETRSYLASGTSRIIVEIGQPVQRGEVLTEGSIEPKNYL
                     SVAGLNATESYLLKEVQKVYRMQGVEIDDKHVEVMVRQMLRKVRIIEAGDTKLLPGSL
                     VDIHNFTDANREAFKHRKRPATAKPVLLGITKASLETESFLSAASFQETTRVLTDAAI
                     KGKRDDLLGLKENVIIGKLIPAGTGMRRYSDVKYEKTAKPVAEVESQTEVTE"
     gene            complement(17201..20752)
                     /gene="rpoB"
                     /locus_tag="PPY92_000537"
     CDS             complement(17201..20752)
                     /gene="rpoB"
                     /locus_tag="PPY92_000537"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDJ2624561.1"
                     /translation="MAGQVVQYGRHRKRRNYARISEVLELPNLIEIQTKSYEWFLREG
                     LIEMFRDISPIEDFTGNLSLEFVDYRLGEPKYDLEESKNRDATYAAPLRVKVRLIIKE
                     TGEVKEQEVFMGDFPLMTDTGTFVINGAERVIVSQLVRSPSVYFNEKIDKNGRENYDA
                     TIIPNRGAWLEYETDAKDVVYVRIDRTRKLPLTVLLRALGFSSDQEIVDLLGDNEYLR
                     NTLEKDGTENTEQALLEIYERLRPGEPPTVENAKSLLYSRFFDPKRYDLASVGRYKTN
                     KKLHLKHRLFNQKLAEPIVNTETGEIVVEEGTVLDRRKIDEIMDVLESNANSEVFELH
                     GSVIDEPVEIQSIKVYVPNDDEGRTTTVIGNAFPDSEVKCITPADIIASMSYFFNLLS
                     GIGYTDDIDHLGNRRLRSVGELLQNQFRIGLSRMERVVRERMSIQDTESITPQQLINI
                     RPVIASIKEFFGSSQLSQFMDQANPLAELTHKRRLSALGPGGLTRERAQMEVRDVHYS
                     HYGRMCPIETPEGPNIGLINSLSSYARVNEFGFIETPYRKVDLDTHAITDQIDYLTAD
                     EEDSYVVAQANSKLDENGRFMDDEVVCRFRGNNTVMAKEKMDYMDVSPKQVVSAATAC
                     IPFLENDDSNRALMGANMQRQAVPLMNPEAPFVGTGMEHVAARDSGAAITAKHRGRVE
                     HVESNEILVRRLVEENGVEHEGELDRYPLAKFKRSNSGTCYNQRPIVAVGDVVEYNEI
                     LADGPSMELGEMALGRNVVVGFMTWDGYNYEDAVIMSERLVKDDVYTSIHIEEYESEA
                     RDTKLGPEEITRDIPNVSESALKNLDDRGIVYIGAEVKDGDILVGKVTPKGVTELTAE
                     ERLLHAIFGEKAREVRDTSLRVPHGAGGIVLDVKVFNREEGDDTLSPGVNQLVRVYIV
                     QKRKIHVGDKMCGRHGNKGVISKIVPEEDMPYLPDGRPIDIMLNPLGVPSRMNIGQVL
                     ELHLGMAAKNLGIHVASPVFDGANDDDVWSTIEEAGMARDGKTVLYDGRTGEPFDNRI
                     SVGVMYMLKLAHMVDDKLHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGA
                     AYTLQEILTYKSDDTVGRVKTYEAIVKGENISRPSVPESFRVLMKELQSLGLDVKVMD
                     EQDNEIEMTDVDDDDVVERKVDLQQNDAPETQKEVTD"
BASE COUNT         6285 a         4111 c         3255 g         7256 t
ORIGIN      
        1 catgattaac ttttacatat tgttttcatg attatattca atttttatta atattttggt
       61 acaacgactc tccaaccatt tttatcttct aaagtaccat tttgaatacc agtatagacg
      121 tcgtataatt tttgagtaat ttcaccagtc tcattattat taataacgat ttcacgatct
      181 tcgtatctca atgtacccac aggtgaaata actgctgcag taccactacc aaatacttct
      241 gttaactcac ctttatcata tgattcgaat aattcatcga ttgaaacgcg gcgctcttcg
      301 acttcatatc ctaagttttt agctaattcg ataatagatt tacgtgtaat accaggtaaa
      361 atactgccat tcaactctgg tgtaattact tttccatttt caacgaagaa aatattcatg
      421 ctaccaactt cttcgatata tttctgttca acaccatcaa gccataatac ttggtcataa
      481 cctaatttat ttgcatttgt ttgtgctaat aaacttgccg catagttacc tgcaactttt
      541 gcaaagccta caccgccacg aacagcacgc acatattcat cttctacata gattttagtt
      601 ggttttaaag tctcaccacc ataatatgca cctgaaggag ataaaataat taataattta
      661 tactgatgtg atgcaccaac gccaagtgcc ccttctgttg caaaaacaaa tggacgaata
      721 tataatgatt gaccttcccc ttcaggaatc caatctcttt ctatatcaac taattgtttt
      781 agcccctcta acaattctgc ttcgtctact tgaggcattt ctaatcgtgc taacgagtta
      841 ttaagacgct taaaattttc ttcaggacgg aaaagtgcaa cttccccatc tcttttatat
      901 gcttttaatc cttcgaatac cgattgacca taatgaacac cttgtgcagc aggtgaaatt
      961 tcaataggac cataaggtac tatcttcaaa tcatgccatc ctttatctgc atcataatca
     1021 taactcaaca tataatcagt aaaatattta ccaaaaccta gttgagatgt atttggtttt
     1081 tgttttaatg tttctcgtcg ttcaacttta actgcttgtg acatggtgat tgcctcctaa
     1141 taatattgta taagaatttg tttaacttaa attataacaa tccatatttt gctgttcaat
     1201 aaattttcta aaaattcaaa attaattaac agattttcta gaaagactat atcttttagt
     1261 ataaacgtat taatttcaca gagacaagta atctgtgttt tactaatata ctttacatac
     1321 aaaaaactct ttactttaaa atgaactaag ctcgcgaatt caatatgtat aatgaataat
     1381 attagaattc atgcactagt ttattaaaat aaagagtaat ttaaaatatc attccgtgta
     1441 ttaaagtgaa tggaaatgat tagttattat ttttaacagt atctttttgt tcaatagctt
     1501 ctaacattaa tttagtcatg ctcgctaaat catatttagg atcaaatccc cattcgccac
     1561 gtgaacagct tgtatcaata gaatccggcc aactattagc gataccttgt ctaataggat
     1621 caacatcata atctaatgta aaatttggat agtattcttg aattgcttct tttaccatct
     1681 ctggatcaaa actcattgcg ctcaaattat aaccgtttct agtttctaat ttagcgtcgt
     1741 ctgcttccat aagtttaata attgcttcaa ttgcatcatc catatacatc atatccatat
     1801 acgtgccttt atctatgaag cttgtataat gaccctctct tactgctttg aagtatattt
     1861 caacagcata gtctgtagta ccgccacctg gctctttaac atgcgagatt aaacctggga
     1921 atctaacact tcttgtatct acaccaaaac gtttgaaata gtattgacac aataattctc
     1981 cagctacttt atttacacca tacattgtcg taggttgctg aatcgttact tgtggcgtat
     2041 taactttagg agttgagtct ccaaatgcac caattgaact tggtgtgaaa aagtgcaaat
     2101 tataagttct tgcagcttct aatgcattca ttaatccacc catatttaaa tcccaagcta
     2161 gaattggatt tttctctgca gttgctgata ataatgctgc catatgcatt agactatccg
     2221 cttcaaagtc cctaactaac tcaaacatgc ggtcacgatc tgttacgtct aaaatttcaa
     2281 atggtccatt ttgtacaggt gagtctgctt caggttccct aatatctgta gcaagaacat
     2341 tatctgtccc ataaatttct ctgcacttaa caactaattc tgtaccaatt tgtcctaatg
     2401 caccagtaat cataattttt ttcatagaaa tatctccttt gtctcttcta tatagctata
     2461 gtccgtcaca agcggacata atattcattt tcataataat tataatataa aagcgctttc
     2521 ttgtatatat gacatgtaca tgttgctgat gtttctgtaa atggaaattc tagttgtatt
     2581 aattgatttt agtaatttat agcgtttatt attgctaatt actgtcaaat tatatttttt
     2641 atccctcaac tcttaaactc tggatatctt tcattatatt agctttttta taaccatgga
     2701 tatcatgtaa agccttataa gctttaaata atgtttcata cctttgtact ttttccgctt
     2761 ctggattaat ttcgtataaa attggctctt tcatagcatc aactgcatca ttaattgaat
     2821 tatgtgcgcc accactgact gcacctaata ttgctgcgcc tattgctggt gcatactcac
     2881 tatccataac aattagtttt ttattcagta cattcgcata gatatccatc ataacagcat
     2941 tctttttagg tataccgcca catgcaaata ccttttctac ttccatatgc caatcttgat
     3001 actgttgcat aatcatctta gtaccaaatg ctgtagcttc taaatatgct ctataaatat
     3061 cctcatgctt agtttgtaaa gttaatccaa agatacaacc tgttaaattg ctatcactta
     3121 atacacttcg atttccatta tgccaatcaa gagcaatgag cccactttca cctggcattt
     3181 gatgttttat cttttcattc attaattcaa atacagtcat atttctattt gctgcttcat
     3241 ctacatatga ctttggtgct tgcttagcga catactcaaa caaatcacct actgctgatt
     3301 gccccgcttc ataagcaaat aattctggaa taattgctcc ttttacagaa cctgatatac
     3361 ctggcacttg atgttgcttt tcatttaaca taagatggca tgtgcttgtt cccatcacca
     3421 tagtcatttc tttatctttt tcagacccaa tacctaataa actagcatgg gcatcaataa
     3481 taaaaggact taccatagtc tcttttgata atcctaattt ctgtgccatt ttatcgtcca
     3541 gtttccctac tacttcacca atattaacaa ccggtgcaga tactttatct tgaattactt
     3601 ttgataattt ggggtctatt ttatcaaata aatcataatg aaaccctgtt tcttcttccc
     3661 aaaatgcttt gaaacctaat ccacaattcg agcgtacatt tttattagtt aatttattta
     3721 caatccaatc gcccgcttcc ataatataag ccgttttttc cataatttct ggtgctcgat
     3781 tcatgacttc cattattttg ggaatcatcc attcactact aacattatat ccataatggc
     3841 ctaaccactt attattattt tcaatagcag tttgatataa tttctctgct tctttatatg
     3901 caccatgatg tttccaaagt ttcacatacg catgtggatt gtttttaaat tgttttaaat
     3961 tatgtaccgg gttaaggttt tcgtcagtaa aaataatagt agatgaagta aagtctatac
     4021 caatacctac tatattgact ggatctattt ttgattcacg tactatatat gatattcctt
     4081 cttccataat ttctaaataa tcattactat tttgaagtgc atatgtatgt ggtattttca
     4141 aaccatttaa ttcactttca atgacaccat gtgtatatgg tttcacaaat tttgatacta
     4201 cttgaccgtt agttgtatta attaaaaaca cacggcctga agcagttcca taatcaattc
     4261 caatgctata cgacatcatg tttcctccat atcgctagtt gcggttatta gttttgcaag
     4321 cgttttctta aatcatactc ataataggtt ctattttaca agtatcactt aaattttgcc
     4381 aaaaaaaaga gtcctcatac atttacatat gaggctcctc taattaatat ttaattattt
     4441 attttgtatt gcatttaaca tttcattaac tgctaatttt cctaactcat ttgaagctaa
     4501 aggactgtca cctgttaata atttacgatc ttttaacgtt cttcctgtca tatcgtcgtt
     4561 aactactttt aatccttgtt tagttaataa atcagcaact aaccatttca agcgtccagg
     4621 taaataacct atttcaatat ttgcaccttc atctaatgag tcagggaaga cacaaacaga
     4681 gtatccttct aatggagatt tttctctgtt aagccctgct gaaagtagtg ctgctggtcc
     4741 atgacataat gtaactataa agcggtcatt gtctaatgcc caatctaatg tttgttgaac
     4801 gtcctcactt tcagaaatac caacaactgc agcatgtccg cctgggataa agacagataa
     4861 atagtctgaa tcaggtccta attcattttt aatcacatct gctaattttt ttggctgttt
     4921 taatttttct ttcaatttat tataagtact tataactgcc tcgtcttcag ttggcatagc
     4981 ccataattct aatttaactg gataaccaga taatgtcgca acatcaacgt caaaacctgc
     5041 ttccattaaa tgatgtaaag gtaataacat ttcaacagga tgattacccg tagagaacat
     5101 ttttccattt tccaataata catatctctc ttcagcagca atcatcaata ctttccattt
     5161 accatcttta taggcacctt tgtgttcaac accatcaaaa tctgttttag gtgctgtata
     5221 ttgactaagg gaatatggtg atgggaaaaa tgcgttatct tctgctttat ctggcgttgg
     5281 ttgcttactt aattcattta catcttgtga catagtttat tcctactttc tctatatagt
     5341 tactactagt atagacatat actacacact ataaacatgt attactatta aaatactcat
     5401 tatcaaaaat aatcaaaatg aatttctttg taaaaaatac aactaaattc tacaagatgc
     5461 ttttaaaatt caaacttcaa ttttggggag cactaaacat tgttataaga acttactgtt
     5521 aataaactaa acattaaaaa aagcccacga caatatttgc cgtgggaata aataaataaa
     5581 tattaaatca acttcatttc ttttcctact ttttcataag ccgcaattgc ttcatctaac
     5641 atgtcttttg tatgtgcagc tgtaggcata tttcttacac gtcctgtacc tcttggtact
     5701 gttgggaaaa cgatagattt cacatagaca ccttcgtctt ttaaacgctt actaaattct
     5761 tgagttgttt tttcatcacc aataattact ggtgtaattg gagtttctga ctcacctgta
     5821 tcatatccta attttgacaa tccatttttt aaatattgtg cattgtccca taatttatca
     5881 tgtaattcag ttgaatccat taacttttta actgcttcag ttattgcttt ggtatcccca
     5941 ggtgctaatg atgtagagaa taagaatggt cgtgattgtg cttttaacca atctattaac
     6001 tcttttgtac ctgctacata gccgccaacg acaccaattg ctttagaaag cgtacctatt
     6061 tggaaatcga ttttatcttg taaaccaaaa tgtttaaccg taccagcgcc tttacccata
     6121 acacctgaac catgagcgtc gtcaacataa gttaataaac caaattcttc tgcaatttct
     6181 acaatttcag gtaatttagc cacatcacca tccatactaa aaacgccatc agtgatatac
     6241 atcactttat tgtattgacc tgattcaact gcttcttttg ctttcgcacg taaatcatcc
     6301 atgtctgaat ggttaactcg aataatttta gctttagata agcgacatcc atcaataatt
     6361 gatgcatgat taagctcatc tgataaaata gcatcatttt tattcatgac agctgaaata
     6421 gcagccatat tacaattaaa tcctgactga taagctattg cagcttctgt tcctttaaat
     6481 tttgctagtg tttcttctaa ttcgtcgtgt aaatctaatg taccattgat tgtacgaaca
     6541 gcgcctgcac ctacaccatg tgtatcaata gctgcttttg cagctgattt caaatcttca
     6601 tttgttgcta gtcctaaata attatttgaa gataagttaa tgtatgattt cccattgatt
     6661 ttgatttctg gtccgtttgc accttcaatt gtatctattt cattatacaa accattttct
     6721 tttagataat ttatattttc ctctaaaaac tcatgtaatg attgaaccac agtatataat
     6781 cccccttaag tattgtttgg taataccttt atcatacagt aattctacat aacttgaaag
     6841 ccttatcatt catagataaa atatctcaaa gcaatcattt agtacgataa tttcataaaa
     6901 tgttatacta taaacattat tttagaaagt agtgataagg gtgttagatt ggttccaact
     6961 agcaaataat aaagaaaata aaacaattca actcagacgt tatttacacc aatatcctga
     7021 gttatctttt gaagaatttc aaacacatga ttatattgtt aaccaattaa gccagttatc
     7081 ttgtgatatt gaaactccta tcggccgcaa tggtatcaaa gccacattta aaggcttggg
     7141 cacaggacct accattgcat taagagctga ctttgatgcg ttacctgtcg aggaattaaa
     7201 cgatgtgcct tataagtcta aaaatccagg atgtatgcat gcgtgtgggc atgatggtca
     7261 tacagcaatt ttacttactg tagcagagat tttagatgaa cataaacatt tattagaagg
     7321 taatgttgta ttaatattcc agtacggtga agaaatcatg ccaggtggtt ctcaagaaat
     7381 gatagatgct ggatgtctgg aaaatgtaga tagaatttac ggtacacatt tatggagtgg
     7441 ttatcctact ggaactattc attcacgtgc gggggcaatc atggcctctc cagatgaatt
     7501 tagcgtcaca attaaaggtc gtggtggtca tggtgccaaa cctcatgaaa ccattgaccc
     7561 aatagtaatc atggcagagt tcattttaag tgcacaaaaa ataatttcac gtacgatcga
     7621 tccagttaaa caagctgtac tttcatttgg aatgattcaa gcaggtacca cagatagcgt
     7681 tattcctgat caagcatttt gtaaaggtac agttagaact ttcgattcag atatacagaa
     7741 tcatgtaatg gacaaaatgg ataagcttct acaaggttta gcaatagcaa atgatattaa
     7801 ctatgatttg aattatatta aagggtattt gcctgtccat aataacgaga aagcatatca
     7861 agttattaaa gaagcaacaa atgacttaca tgtacgcttt aatgaatcag atttaatgat
     7921 gattggagaa gatttttcac attatttgaa agtacgacca ggtgcattct ttctgactgg
     7981 ttgtggtaat gaaagtaaag gtattacagc gccacaccat aatcccaaat ttgatattga
     8041 tgaaaaatca ttaaaatacg ctgtagctgt atttttaaaa atcattgaat tagagcaagt
     8101 atttaaaaca aattaagcat ttaaattaat tgaaagatat gaatctggga cataaactaa
     8161 tgaaatgctc ccttcaaagt agacattgaa aaaatgaaaa ctttgaaggg agcattttac
     8221 aatgtccaag actcaacaat tttatttaaa cagtattatc agttataagt tttattagtc
     8281 agtctttcat caattattgt tcttctaaat cgaatagcca ttacaaaatt tacacattaa
     8341 aaaaagaccc ctcgattgag ggatcttctt tttatataaa atctaagaaa ttagaaatta
     8401 tttaatgatt tcagtaacaa cgcctgatcc tacagtacgt ccaccttcgc ggattgagaa
     8461 acgagtaccg tcttcaatcg cgattggagc gattaattct actgtcattt caacgttatc
     8521 accaggcatt accatttcag taccttctgg taagtgaaca acaccagtta cgtcagtagt
     8581 acggaaatag aattgtggac gatagtttga gaagaatgga gtgtgacgtc caccttcgtc
     8641 ttttgataat acgtatactt ctgctttaaa ttcagtatgt ggtgtaattg aaccaggagc
     8701 agctaatact tgaccacgtt gtacgtcttc acgagcaaca ccacgtaata atgcaccaat
     8761 gttgtcacca gcttcagcgt agtctaataa tttacggaac atttcaacac ctgtaacagt
     8821 tgttttagat gtgtcatgta aaccgatgat ttcaacttct tcaccaactt tgatttgacc
     8881 acgttcaaca cggcctgtag caacagtacc acgaccagtg attgagaata cgtcctcaac
     8941 tggcatcatg aatggtttgt cagaatcacg ttctggagtt ggaatgtaag tatctacagc
     9001 ttccattaat tctaagattt tttcttcgta ttgagcatcg ccttctaaag cttttaatgc
     9061 tgaaccagcg attacaggta catcgtcacc tgggaagtca tattcgctta ataagtcacg
     9121 aacttccatt tctactaatt ctaataattc ttcatcgtca accatgtcaa ctttgtttaa
     9181 gaatactact aatgctggta caccaacgtt acgtgataaa agaatgtgtt cacgagtttg
     9241 tggcattgga ccgtcagcag cagatactac taagataccg ccgtccattt gagcagcacc
     9301 agtgatcatg tttttaacgt agtcagcgtg tcctgggcag tcaacgtgag cgtagtgacg
     9361 tttgtcagtt tggtactcaa tgtgagaagt attgattgtg ataccacgtt ctttttcttc
     9421 tggagcgttg tcaatcatgt catatgattg tgcaactgag tcaccatttt ttgctaatac
     9481 agtagcgatt gctgctgtta atgttgtttt accatggtca acgtgaccga tagtaccgat
     9541 attggcatgt tctttagaac gatcgaattt ttcttttgcc attataaaat ctctcctatt
     9601 ctaattaaaa ataatattta aatttatctc tcatgatagt ttctcaccat catgagaaat
     9661 tatttatagc gatgcaaaaa gaattttaca attcttttat aatgcattca ccccaaaaaa
     9721 tcaaactata aagatgatag cttacattta agctcacctt gtattttaac ctaggctagc
     9781 tagtcaaaac aagttatatt attcaccttt atttttcttg ataatatctt cagcgattga
     9841 ttttggaact tcagcatagt gatcgaagta catagtgtaa gtaccgcgac cttgagtgtt
     9901 tgaacgtaat gatgttgcat aaccgaacat ttctgaaagt ggtacataag cattaacaac
     9961 ttgtgcatta ccacgaggtt ccataccatc aacacgtcca cgacgagatg ttacgtcacc
    10021 catgatatca cccatgtact cttcaggcat ttcaatagtt actttcatca ttggttctaa
    10081 gattacagga tcacattttt tagcagcttc tttaagtgct aatgatgcag caattttgaa
    10141 ggccatttca gatgaatcga catcatggta tgaaccatca tataatttag ctttaacatc
    10201 aattaaagga taacctgcta agacaccatt ttccatagca tctttaagac cagcttctac
    10261 tgatggaatg tattcacgag gaactacacc accaacgata gcgttttcga attcgaaacc
    10321 tgcgcctgtt tcgtttggtg tgaattcaat gtgaacatca ccgtattgac cacgaccacc
    10381 agattgacga gagaatttac cttgaacttg tgcagatgat ttgaatgttt cacgatatga
    10441 aaccattgga gcacctacgt tacattcaac gttgaattct ttcttcatac ggtctactaa
    10501 gatgtctaag tgaagctcac ccataccacc gatgataact tgtccagttt cttcgtcagt
    10561 gtgtgcatgg aatgttgggt cttcttcttg taatttaact aaagcttgag tcattttatc
    10621 ttggtcagct ttagattttg gctctactga taagtgaata actggctctg ggaattccat
    10681 tgattccaag ataatgtcat ttttctcacc acataaagta tcaccagtac ctgtatcttt
    10741 aagacctacc gcagccgcga tatctccaga gtatacagta tcgatttctt gacgtgagtt
    10801 agcgtgcatt tgtaataaac gacctacacg ttcacgttta cctttagtag agttcttaac
    10861 gtatgaacca gatgtcattg tacctgaata cacacggaag aatgttaatt taccaacata
    10921 agggtcagtc ataactttga acgctaatgc agcgaattca gctgaatcgt ctgctttcgc
    10981 gattacttct tcttcagggt tgctagcacg gtgaccaata attggtttaa cgtctagtgg
    11041 tgaaggtaag taatcaatta cagcgtcaag cattaattga acacctttgt ttttgaaagc
    11101 tgtaccacaa agtactgggt agaattctac gttagtagta gcttggcgga tagcttcttt
    11161 taattcagaa actgaaattt cttcgtcacc aagatatttt tccattaatt cgtcgctagt
    11221 ttctgcaact gcttcgatta agctagcacg agcttcttca gctctatcta agtggtcttc
    11281 aggaatttca atttcttcaa tttcagtacc taaatcattt gtatatttga aacatttcat
    11341 ttcaactaag tcaatgattg cttcgaattc gtcttccgca ccaattggta attggattgg
    11401 agcagcgtta gcttgtaaac gatcatgtaa tgtacttaca gagtattcga agttagcacc
    11461 taatttgtcc attttgttta caaatacgat acgtggaaca ccataagttg tagcctgacg
    11521 ccaaactgtt tcagtttgtg gttcaacacc tgattgtgca tcaagtactg taactgctcc
    11581 gtcaagtaca cgtaatgaac gttcaacttc tacagtgaag tctacgtgtc caggtgtatc
    11641 gataatgttt acacggtgac cttcccaagc tgctgttgtt gcagcagatg tgatagtaat
    11701 accacggtct tgttcttgct ccatccagtc catttgtgaa gcaccttcgt gtgtttcacc
    11761 aattttgtgg atacggccag tgtaataaag aatacgttca gtcgtagtcg ttttaccagc
    11821 atcaatgtga gccatgatac cgatattacg agttttttct aatgaaaatt ctctagccat
    11881 gtattttttc tccttccagt ttatattact gaataaatac gatagatatg gcgatgccct
    11941 aagcatgaac gcttaatgaa aattcattaa tatagaacac actcagggta aaagctttta
    12001 tcttaccaac ggtagtgagc aaatgctttg tttgcttcag ccattttgtg agtgtcctca
    12061 cgtttcttaa cggcaccacc tgtattattt gctgcatcta aaatttcgtt agctaaacga
    12121 tcttccatcg ttttttcacc acgaagacgt gcatagttaa ctaaccaacg taaacctaaa
    12181 gtagtacgac gctctggacg aacttctact ggtacttgat agttagaacc acctacacga
    12241 cgagctttaa cttctaatac tggcataatg ttgttgattg cttcttcgaa tacttctaat
    12301 gcatcacgac cactgcgttg ttcaactagg tcgaatgctg aataaagaat tctttgtgct
    12361 gttccacgtt taccatctaa cataattttg ttaattaatt tagttactaa cttagagtta
    12421 tgaattggat ctggtaatac gtctctttta ggtactgatc ctttacgagg cataatgtaa
    12481 atcctccctt ccttatatta aatattttaa gtttaagatt taattaaaaa ctaaattctt
    12541 agtttttagg tttcttagtt ccgtataatg aacgaccttg tctacgtccg tcaacacctg
    12601 aagtatcaag tgctccacgt acaatatggt aacgcacacc tggtaagtct tttacacgtc
    12661 caccacgtac aagtacaaca ctgtgttctt gtaagttatg tccgatacca gggatgtatg
    12721 cgttaatttc gatgttgttt gataaacgca cacgtgcata tttacgtaac gctgagttag
    12781 gttttttagg tgtcattgta cctacacgag tacatacacc acgtttttgt ggtgagttta
    12841 agtcagtaaa ttttttcttt ttactgttga aacctttatt taaagctgga gaatctgatt
    12901 ttttgatttt gctttgtctt ggtttacgta ctaattggtt aatagttggc atgtgatatg
    12961 tcctcctctc ttcacttttt aatcccacac atccaggtgg ttcattttta ggttaaataa
    13021 aatttagtaa gtaaaacact tactaattct catttaatca atgctactat tgtcgcattg
    13081 acgttaatac ctacatgttt acccaaagca tgtttgcttt tgaaaaaaga tacaggtata
    13141 tttttctgat tgatttggct taacacgcga gtcattaaat atacttcaac gtcttcagca
    13201 ataatcaaag atgtaacttg atctttcttt aacgctttaa gcgtttcttt aagaccaact
    13261 acaaaatgtt gtttgttaaa gcgtgcaact ttttccttag acaataattt atcctccaaa
    13321 gttctgcttg catcaacctt taatatttta acattgaaca agagaattcg tcaacattaa
    13381 atcaaataat ttacataaaa ataacaagag gctaaagcat tagcctctgt tatatacttg
    13441 ttattccgtt acttcagttt gagattcaac ttctgcaact ggtttagctg ttttttcgta
    13501 ttttacgtcg ctataacgtc tcataccagt accagctgga attaacttac caataattac
    13561 gttttcttta agacctaata agtcatcacg cttaccttta attgctgcat ctgtaagaac
    13621 tcttgttgtt tcttggaatg atgctgcaga taagaaactt tctgtttcaa gtgatgcttt
    13681 agtaatacca agtaatactg gtttagctgt tgcaggacgc ttacggtgtt taaatgcttc
    13741 tctatttgca tctgtaaagt tatgaatatc aactaatgaa cctggtaata acttcgtatc
    13801 acctgcttcg ataattctaa ctttacgtaa catttgtcga accataacct caacgtgttt
    13861 atcgtcgatt tctacacctt gcatacggta aactttttgt acttctttta ataagtagct
    13921 ttcagtcgcg tttaatccag caacagataa gtaattctta ggttcaatag aaccttcagt
    13981 taatacttca ccacgttgaa ctggttgacc gatttctaca ataattcttg aagtacctga
    14041 agcaaggtat gatcttgttt cattagcacc tttaacaaca atttcttgtt gtctatcttt
    14101 tgctaattta atatcttcta cgacaccttc gatttccgta attaccgctt gacctttagg
    14161 gttacgtgct tcgaaaatct cttgaatacg aggaagacct tgtgtgatat cgctacctgc
    14221 taccccacct gtatggaatg tacgcattgt aagctgtgta cctggttcac cgatagattg
    14281 ggctgcaatt gtaccaactg cttcaccaac ttcaactttt tcaccagtag caaggttttt
    14341 accgtaacat ttttcacaaa caccatgtcg tgcgttacaa gtaaatgctg agcgaatata
    14401 catttgttca ataccagcat ctgtaatttt cttagcaatt tcaggtgtaa ttaattcatc
    14461 aggacgaatg attatttcat cagtttcagg atgacgaatt gtttctttag aataacgacc
    14521 ttcaatacgt tcgataaatg gttcaatcat ttctgtacct tctttaatat cagaaactaa
    14581 taaacctcta tcagtaccac agtcttcttc acgaacaata acatcttgtg ccacgtcaac
    14641 aagacgacga gtaagatatc ctgagtcagc tgttttaagt gctgtatcgg caagaccttt
    14701 acgtgcaccg tgagttgaga tgaagtattc aagtactgtt aaaccttcac ggaatgaaga
    14761 tgtgattggt aattcgataa tcttaccaga tggtgcggcc atcaatccac gcatacctgc
    14821 taactgtgta aagttagatg cgttaccacg ggcacctgaa tcactcatca tgaagattgg
    14881 gttagtttta tcaagtgatt gcatcaattc accttgaatt tgatcttttg catctgtcca
    14941 aatttcaaca actgcattat atctttcttc ttcagtgatt aaaccacggt tgaattgttt
    15001 tgtaattctg tcgactaatt tttcatgctc atcaagtatt tgttgcttat caggtaatac
    15061 tacgatatca gcaacaccta ctgtaatacc agctttagat gagaatttga atcctaagtc
    15121 tttcatacgg tctaacatca ttgatgtatc agtgatgcta aatctgttga atacttctgc
    15181 aataatatta cctaagaatt ttttgttgaa aggttcaatt aattcttcat tttcaaagta
    15241 ttcttttaat ccaccttcac ctaaagttgt aggatcgatg aaatatctgt ttggtgtctt
    15301 tctttctaag ttttcttgcg taggttcatt aatataagca aatgaatctg gaatgatttc
    15361 attgaatata attttaccta ctgacgtagc aagaatcttt ttgttttgtt cttcagtaaa
    15421 tgttggatta ttgaacgaac ttgcatgtac accaattcta gtgtgtaaat gtacaaagcc
    15481 atttgcatat gcttttaata cttcatttgt attattaaag attgcgcctg tatttactgc
    15541 atcttttctt tctaaagtaa ggtaatagtt accaagtacc atatcttgtg atggtgtaac
    15601 tacaggttta ccatctttag ggttcaagat gttttgtgct gctaacatca acattcttgc
    15661 ttcagcttgt gcctcttttg ataaaggaac gtgaaccgcc atttggtcac cgtcaaagtc
    15721 agcattataa gctgttgtta caagtggatg tagacgaatc gcacgacctt caactaaagt
    15781 tggttcaaat gcttgaatac caagtctatg aagtgttggt gcacggttaa gtaatacagg
    15841 atgttctcta attacttctt ccaatacgtc ccaaacttca tcatccatac gttcgatttt
    15901 actcttcgca tttttaatgt tagttgcaat ttcacgttga actaattctt tcattacgaa
    15961 tggtttaaat agttcaagtg ccatttcttt tggtaaacca cattggtaca ttttcaagct
    16021 tggacctact gcaataactg aacgtcctga atagtcgaca cgtttaccaa gtaagttttg
    16081 acggaaacga ccttgtttac cttttaacat atgagataaa gattttaatg gacggttacc
    16141 tgggccagta actggacgac cacgacgacc attatcaatt aaagcgtcaa cggcttcttg
    16201 taacatacgt ttttcgtttt gaacgatgat accaggtgca cctaaatcta ataaacgttt
    16261 caaacgatta tttcgattaa ttacacgacg gtataagtcg tttaagtcac ttgttgcaaa
    16321 tcgtccacca tctaattgaa ccattggacg aatttctggt gggatgattg gaagtacatc
    16381 taaaatcatc catgaaggtt tgttacctga attacggaat gattcaacaa cttctaaacg
    16441 tttaattgca cgagtaagtc tttgaccagt agctgattcc aactcatcgc gtaacaattt
    16501 aagttcttcg tcaagatcaa tctcttcaag taaatcttta ataccttctg cacccatttt
    16561 tgcaacgaat tgacctgggt atttatcata ataatctctg aattcagctt cagataataa
    16621 agttttcttt tctaaaccag ttggacctgg atctacaaca acataagaag caaagtaaat
    16681 aacctcttct aatgctcttg gtgacatgtc aagtaataat cccatacgac ttggtatacc
    16741 tttgaaatac caaatgtgag aaactggagc agcaagttca atgtgaccca ttctttcacg
    16801 acgtacttta gatttagtta cttcaactcc acatctgtca cagaccatgc ctttgtagcg
    16861 aacacgtttg tatttaccac aactacattc ccagtctttt gtaggtccga aaattctttc
    16921 acagaataga ccatcttttt caggttttaa tgtacggtag ttgattgttt caggtttttt
    16981 aacttcacca aaagaccaag aacggatttt ttcaggtgaa gccaatccta ttttcatata
    17041 atggaaatta tttacatcaa tcaaggagcc tacctccttc aatttagatt agctgtgcta
    17101 tttgattgat ttgcatttat ttaaaatggc ttaaagtact aaactgaatc atcaataaaa
    17161 ttcagcttag tatctttttg cctgttttgt aaattgcgta ttaatcagta acttcttttt
    17221 gtgtttcagg agcatcattt tgttgtaaat ctactttgcg ttctacaaca tcatcgtcat
    17281 caacgtctgt catttcgatt tcattatctt gctcatccat aacttttaca tctaaaccta
    17341 aactttgtaa ttctttcatc aatactcgga atgattctgg aacacttggt ctagagatgt
    17401 tttcaccttt aacaatagcc tcgtatgttt tcacacgtcc tactgtatca tcggatttgt
    17461 aagttaagat ttcttgtaat gtgtatgcag caccatatgc ttcaagtgcc catacctcca
    17521 tctcaccgaa acgttgtcca ccgaattgcg ctttaccgcc aagtggttgt tgtgtaacaa
    17581 gtgaatatgg tcctgttgaa cgcgcatgta atttatcatc aaccatgtgc gcaagtttca
    17641 acatgtacat tacacctact gaaatacggt tatcgaatgg ttcacctgta cgtccatcat
    17701 aaagtacagt tttaccatca cgagccatac cagcttcttc aattgttgac catacatcgt
    17761 catcgtttgc accgtcaaat actggtgatg caacgtgaat accaagattt ttagcagcca
    17821 tacctaagtg tagctctaat acttgtccga tgttcatacg agatggtaca ccaagaggat
    17881 ttaacatgat atcgatcgga cgtccatctg gtaagtaagg catatcttct tcaggaacaa
    17941 tcttagaaat gacacctttg ttaccatgtc gaccacacat cttatcacca acatgaattt
    18001 tacgtttttg aacgatatat acacgtacta attggtttac accaggtgat aatgtatcgt
    18061 cgccttcttc acgattgaat acttttacat caagaacgat accgccagcg ccgtgaggta
    18121 cacgtaatga agtatctcta acttcacgtg ctttttcacc aaagattgca tgtaacaatc
    18181 tttcttcggc agttaactca gttacacctt taggcgttac tttaccaact aaaatatctc
    18241 catcttttac ttctgcacca atataaacga taccacgatc gtctaagttc ttaagtgcac
    18301 tttcagaaac attaggaata tctcttgtga tttcttcagg tcctaactta gtatcacgtg
    18361 cttctgattc atactcttca atatgaatag aagtatacac gtcatctttc acaagtcttt
    18421 cactcatgat aacggcatcc tcatagttgt aaccgtccca agtcatgaaa ccaactacta
    18481 cgtttctacc taatgccatt tctcctaatt ccatagatgg tccatctgct aaaatctcgt
    18541 tatactcaac aacatctcca actgcaacga ttggacgttg gttgtaacat gtacctgagt
    18601 ttgaacgttt aaatttagct aatggatagc gatctaattc accttcatgc tcaacgccgt
    18661 tctcttcaac tagacgacgt acaagaattt cattagattc aacatgttca acacgacctc
    18721 tgtgcttagc tgtaatagct gcaccagaat cacgtgctgc aacgtgttcc atacctgttc
    18781 caacaaatgg tgcttctgga ttcatcaaag gcactgcttg acgttgcatg ttcgcaccca
    18841 tcaatgcacg gtttgagtca tcgttttcta agaatggaat acatgctgtc gctgctgaaa
    18901 caacttgctt cggcgataca tccatataat ccattttttc tttagccata actgtattgt
    18961 taccacggaa acgacataca acttcatcat ccatgaaacg accattttca tctaatttag
    19021 agtttgcttg tgctacaaca tagctatctt cttcgtcagc tgttaaatag tcaatttgat
    19081 cagtgatagc atgtgtatct aaatcaactt tacgatatgg tgtttcaata aagccgaatt
    19141 catttacacg tgcataactt gataatgagt taatcaatcc aatgtttggt ccctcaggtg
    19201 tttcaattgg acacatacgg ccatagtgag agtagtgaac gtcacgtact tccatttgag
    19261 cacgttcacg tgttaaacca ccaggtccta atgctgatag acgacgttta tgcgttaact
    19321 cagctaatgg gtttgcttgg tccatgaatt gtgataattg agagctacca aagaattctt
    19381 taatagatgc aataacaggt cgaatattaa ttaattgttg aggtgtgata gactcagtat
    19441 cttgaattga cattctttca cgtacaactc tttccattct tgataaaccg atacggaatt
    19501 ggttttgtag taattcacct acagaacgta aacgacggtt acctaaatgg tcaatatcat
    19561 ctgtatatcc aataccgctt aataagttaa agaagtaact cattgaagca atgatatctg
    19621 ctggtgtaat gcatttaact tctgagtcag ggaaagcatt accaattaca gttgtcgtac
    19681 gaccttcatc atcgttagga acatatactt taattgattg aatttctact ggctcgtcta
    19741 taacgctacc atgcaattca aacacttcgc tgtttgcatt tgattcaagt acatccatga
    19801 tttcgtcgat tttacgacga tcaagcactg taccttcttc aactacaatt tcaccagttt
    19861 cagtatttac aattggctca gctaattttt gattaaataa acgatgtttt aaatgtaatt
    19921 ttttgtttgt tttataacga cccacgcttg ctaagtcata gcgttttgga tcaaagaaac
    19981 gtgaatacaa tagactttta gcattttcaa cagttggtgg ttcacctgga cgtaaacgtt
    20041 catagatttc taataacgct tgttcagtgt tttcagtgcc gtctttctct aaagtattac
    20101 gtaaatattc attgtcacct aaaaggtcaa caatttcttg gtcacttgag aaacctaatg
    20161 cacgtaacaa tactgttaat ggtagtttac gtgttctatc aatacgtacg tatacaacat
    20221 ctttagcatc tgtttcatat tctaaccatg caccacggtt tggaataatt gttgcatcat
    20281 agttttcacg accatttttg tcgatttttt cattgaaata aacggatggt gaacgaacta
    20341 attgagatac gattacacgt tctgcaccat tgataacgaa cgtacctgta tcagtcatta
    20401 atgggaaatc acccataaag acttcttgtt ctttaacttc tcctgtttct ttaatgatta
    20461 gacgcacttt tacacgaaga ggtgcagcat aagtagcgtc acggttttta gattcttcta
    20521 aatcatattt tggttctcct aaacggtaat ccacaaactc taatgacaaa ttaccagtaa
    20581 aatcttcaat tggagaaatg tctctaaaca tttcgattaa accttctctt aggaaccact
    20641 cgtaagattt agtttgaatt tctattaagt ttggtaattc taatacttct gaaattctcg
    20701 cgtagtttct acgtttacga tgtcttccat attggacaac ttgacctgcc aaacagattc
    20761 acccctcaaa aattatgtga tccgctttta gctaaatacc ataagcatat taataaaaag
    20821 acaaaaagaa aacggcaaca ctaatgatga caccattttc gattctatat ttattcttaa
    20881 ttaacttatc tatttaacgt aaaagta
//
LOCUS       DANBWH010000021        20132 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.021, whole genome shotgun sequence.
ACCESSION   DANBWH010000021 DANBWH010000000
VERSION     DANBWH010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20132
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.021"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            11..520
                     /locus_tag="PPY92_000538"
     CDS             11..520
                     /locus_tag="PPY92_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDJ2624562.1"
                     /translation="MTVDNNKAKQAYDNQTGVNEKEREERQKQQEQNQEPQFKNKLTF
                     SDEVVEKIAGIAAREVKGILDMKGGLTDTFTNAFSSGNNVTQGVSVEVGEKQAAVDLK
                     VILEYGESAPKIFRKVTELVKEQVKYITGLDVVEVNMQVDDVMTQKEWKQKHEKNNEN
                     NNQERQGLQ"
     gene            complement(681..2657)
                     /gene="sfaD"
                     /locus_tag="PPY92_000539"
                     /gene_synonym="sfnaD"
     CDS             complement(681..2657)
                     /gene="sfaD"
                     /locus_tag="PPY92_000539"
                     /gene_synonym="sfnaD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine--citrate ligase SfaD"
                     /protein_id="HDJ2624563.1"
                     /translation="MNLNLIFKEQTLKFNKEEQETYLFLQQHNSDWANIFKEMILQGR
                     DKVTQRLVTSMHRENLVKARTQSKKILSRDLIMLDISTTHILEIQFPQAKQTLYAPIT
                     GEHAFDRIDVEGPFYIKDDITNTITRVHHPNEILECILIEAPDLKNAASDQFQQDLIN
                     SATNMTFAISYQALSMQHDSAPLFNIIENSEDSYLRSEQAVIEGHPLHPGAKLRKGLN
                     ALQTFLYSSEFNQPIKLKIVLIHSKLSRTMSLSKDYDTTVHQLFPDLIKQLENEFTPN
                     FNFNDYHIMIVHPWQLDDVLHSDYQAEVDKELIIEAKHTLDYYAGLSFRTLVPKYPAM
                     SPHIKLSTNVHITGEIRTLSEQTTHNGPLMTRILNDILEKDVIFKSYASTIIDEVAGI
                     HFYNEQDEVDYQTERSEQLGTLFRKNIYQMIPQEVTPMIPSSLVATYPFNNESPIVTL
                     IKRYQSAASLSDFESSAKSWIETYSKALLGLVIPLVTKYGIALEAHLQNAIATFRKDG
                     LLDTMYIRDFEGLRIDKAQLNEMGYSTSHFHEKSRILTDSKTSVFNKAFYSTVQNHLG
                     ELILTISKASNDSNLERHMWYIVRDVLDNIFDQLVLSTHKSNQVNENRINEIKDTMFA
                     PFIDYKCVTTMRLEDEAHHYTYIKVNNPLYRENN"
     gene            2759..3952
                     /gene="sfaA"
                     /locus_tag="PPY92_000540"
     CDS             2759..3952
                     /gene="sfaA"
                     /locus_tag="PPY92_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A export MFS transporter"
                     /protein_id="HDJ2624564.1"
                     /translation="MTKYFFSSSFLLFLGNWIGQIGLNWFVLTTYHNAVYLGIVNFCR
                     LVPILLLSVWAGAIADKYDKGRLLRITISSSFLVTAILCVLTYSFTAIPISVIIIYAT
                     LRGILSAVETPLRQAILPDLSDKISTTQAVSFHSFIINICRSIGPAIAGVILAVYHAP
                     TTFLAQAICYFIAALLCLPLHFKVTKIPEDATRYMPLKVIIDYFKLHMEGRQIFITSL
                     LIMATGFSYTTLLPVLTNKVFPGKSEIFGIAMTMCAIGGIIATLVLPKVLKYIGMVNM
                     YYLSSLLFGIALLGVVFHNIVIMFICITLIGLFSQWARTTNRVYFQNNVKDYERGKVL
                     SIIMMDRGMIPLGSLLMSICADVFGIVRTFSIMGISTICITMVFYFINRKLKLKLEES
                     NHGIS"
     gene            3939..5696
                     /gene="sfaB"
                     /locus_tag="PPY92_000541"
     CDS             3939..5696
                     /gene="sfaB"
                     /locus_tag="PPY92_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A synthetase SfaB"
                     /protein_id="HDJ2624565.1"
                     /translation="MVYLEWAKADRNIQYRVINAIIKERIYPEQTFISQKGSLIEIQY
                     HMHVLTIEVVRKSALERYEFTGDITYLNKGETSLIITLEGLLDVLNHDFDIPISERLR
                     EELIYSRDSLVETYKQMSHRQTLISQSFKFSRLPQDINFFSWLQHVKDSDKTDDLTYS
                     ESLVPEGHPTHPLTKTKLPLTMEEVRAYAPEFEKEIPLQIMMIEKDHVVCTAMDGNDQ
                     FIIDEIIPEYYNQIRVFLKSLGLKSEDYRAILVHPWQYDHTIGKYFEAWIAKKILIPT
                     PFTILSKATLSFRTMSLIDKPYHVKLPVDAQATSAVRTVSTVTTVDGPKLSYALQNML
                     NQYPGFKVAMEPFGEYANVDKDRARQLACIIRQKPEIDGKGATVVSASLVNKNPIDQK
                     VIVDSYLEWLNQGITKESITTFIERYAQALIPPLIAFIQNYGIALEAHMQNTVVNLGP
                     HFDIQFLVRDLGGSRIDLETLQHRVSDIKITNDSLIADSIDAVIAKFQHAVIQNQMAE
                     LIHHFNQYDCVEETELFNIVQQVVAHAINPTLPHANELKDILFGPTITVKALLNMRME
                     NKVKQYLNIELDNPIKKEV"
     gene            5703..6773
                     /gene="sfaC"
                     /locus_tag="PPY92_000542"
     CDS             5703..6773
                     /gene="sfaC"
                     /locus_tag="PPY92_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A biosynthesis protein SfaC"
                     /protein_id="HDJ2624566.1"
                     /translation="MAHVNINISKIKYNAKVLQTVFQSKNIQFTPVIKCIAGDRTIVE
                     SLKALGINHVAESRLDNIISIADQDLTYTLLRTPAKKEISDMIEKVDMSIQTELSTIH
                     QINEVAEVLGKKHKILLMVDWKDGREGVLTYDVLDYIKEIIHLKNIHFVGLAFNFMCF
                     KSDAPSDDDIFMINRFVSAVEREIGYRLKIISGGNSSMLPQLLYNDLGKINELRIGET
                     LFRGVDTTTNQAIAMLYQDAITLEAEILEIKPRVNTQTHESFLQAIVDIGYLDTKVDN
                     ISPMDQHINILGASSDHLMLDLNGQGHYQVGDHISFSLNYEALSHSMYMKNLHKVYID
                     DSKIDTLLQNFDVKSPAMVNQY"
     gene            complement(6874..6999)
                     /locus_tag="PPY92_000543"
     CDS             complement(6874..6999)
                     /locus_tag="PPY92_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624567.1"
                     /translation="MYHCYIIFALIQLTPFSMTVIPVIQLILSVAILCDELLHLI"
     gene            7165..8148
                     /locus_tag="PPY92_000544"
     CDS             7165..8148
                     /locus_tag="PPY92_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDJ2624568.1"
                     /translation="MRGLKTFSILGLIVALLLVAACGNTDNSSKKESSTKDTISVKDE
                     NGTVKVPKDAKRIVVLEYSFADALAALDVKPVGIADDGKKKRIIKPVREKIGDYTSVG
                     TRKQPNLEEISKLKPDLIIADSSRHKGINKELNKIAPTLSLKSFDGDYKQNINSFKTI
                     AKALNKEKEGEKRLAEHDKLINKYKDEIKFDRNQKVLPAVVAKAGLLAHPNYSYVGQF
                     LNELGFKNALSDDVTKGLSKYLKGPYLQLDTEHLADLNPERMIIMTDHAKKDSAEFKK
                     LQEDATWKKLNAVKNNRVDIVDRDVWARSRGLISSEEMAKELVELSKKEQK"
     gene            8160..9191
                     /locus_tag="PPY92_000545"
     CDS             8160..9191
                     /locus_tag="PPY92_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDJ2624569.1"
                     /translation="MAIKEISSQSAIDHKRKRRTTLTYIVSLCFLFICIYLNMAIGSS
                     KINFSDIIHYVTGHTDTKATFLLHNVRMPRMIAGLFIGGALAVSGLLMQAMTRNPLAS
                     PKIFGVSSGASFFIVFVTIIIPSLEYYALYLGVIGAFIGGLTVYTLSGATKGMTPIKL
                     ALAGMAIHLFFSSMTEGIIILNENSNEQVMFWLVGSLSSMKWDEILTILPWIIGALIV
                     TIFIGRQLTIMELGDDIAKGLGQNINKVRIIIGLLVIILTGMSVSVAGPIGFVGLIVP
                     HIVKRYVSKNYLVMIPLTFIIGADLLLLSDVLSRLITYPYESPVGIVTSFVGALYFLF
                     ITIKGVKRI"
     gene            9188..10156
                     /locus_tag="PPY92_000546"
     CDS             9188..10156
                     /locus_tag="PPY92_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDJ2624570.1"
                     /translation="MMTTKIIRRYSLITILLIVSIFISLCVGSVMIHPIDAIKGIFTQ
                     DDFILNEYRIPRTLLGIIIGSSLAISGAIIQAVIRNPLASPDVIGISKGASLAAVIII
                     MIFPTAPLFVLPIGSFLGAFAVSLFLSFLISKYDVKGSKLALIGLAIGAICTAIVQFL
                     LIRNPLEANKALVWLTGSLYGHDMSNIYTILPWFLITIPIIILLSYQLDVLNLGDAVA
                     TALGLKVKTIKMLLLILAVILAGSAISVVGGISFLGLIAPHIARQLIGNKTLHVIIMS
                     GLIGAILLTFGDGLARGIHPPLDIPVGVIIAIIGAPYFLILLRRMK"
     gene            10392..11762
                     /locus_tag="PPY92_000547"
     CDS             10392..11762
                     /locus_tag="PPY92_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HDJ2624571.1"
                     /translation="MDSFSRKEIVIGRLKFITMSLIGILLFLVPIPVEQDGQKQTTLP
                     VAFLAGVLKDVLGGVMPFLIVTIITLSGIITLICSTILKDKLKPDGLMNNAFNVRIGW
                     LILRILAVVFAWMTFLRIGSKVIYSDETGGLLFSSLLPTLVAVFLFAALFLPLLMEYG
                     LLEMLGPIFRPVMRPLFTLPGRSTVDNLASFIGDGTVGVLITSRQYGEGYYSRREATV
                     ISTTFSVVSITFAIVVAETVHMQNQFFAFYLSVIVSCLVAAVIMPRIWPLNKIPDEYA
                     KEVPESARTEALPEGKTALRHGFDTATEVGIKAPGVIDFFKSGLKTVIDMWFVILPVV
                     MSIGTIATIIANYTPFFVILGKPFVPFLELMQIPEAAQASQTIIIGFADMFLPSILIE
                     GVQNDITRFVIGALSISQLIYLSEVGGVILGSKIPVSIGKLFMIFLIRTIITLPIISL
                     MAHLLL"
     gene            complement(11831..12091)
                     /locus_tag="PPY92_000548"
     CDS             complement(11831..12091)
                     /locus_tag="PPY92_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624572.1"
                     /translation="MAMTVKKDNNEVRIQWRVADIKIPTSEIKNITQDQDIHAVPKLD
                     SKDVSRIGSTFGKTNRVIIDTEDHEYIIYTQNDQKVYNELTK"
     gene            12268..12783
                     /locus_tag="PPY92_000549"
     CDS             12268..12783
                     /locus_tag="PPY92_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDJ2624573.1"
                     /translation="MTGKTHASCGMLVGALTTQYFQTDIFSSVTVIILATLASLLPDI
                     CHTQSKIGRRFKVISFFVRLIFGHRTFTHSILFIAIIAFLLQIIQTPNYYMAAIIIGL
                     VSHVILDMITPRGVKLFYPLPFNVKTPIQFKTGGLVDLSLATALMVGTVYVLFQPFIN
                     DIISNWNTKFL"
     gene            12827..14014
                     /locus_tag="PPY92_000550"
     CDS             12827..14014
                     /locus_tag="PPY92_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HDJ2624574.1"
                     /translation="MLDKNQLAKYKQDHLCEYEKIMSNNEKEALEEKVASLDLDFIAK
                     LYNDLYINKKTIDDVSAVSEVKYDIKSQMSDDEIKRLEEQGLQAIKEGQFAVLLMAGG
                     QGTRLGYKGPKGSFEIEGVSLFELQANQLKTLNHQSGHTIQWYIMTSDINHEETLAYF
                     EAHSYFGYDQEAIHFFKQDNIVALSEEGKLILNQQGRIMETPNGNGGVFKSLDKAGYL
                     EEMSNNGVKYIFLNNIDNVLVKVLDPLFAGFTVEHDYDITSKTIQPKPGESVGRLVNV
                     DCKDTVLEYSELDPEVANQFNNANIGIHAFKLGFILNAVNRELPYHLAIKNLKQLDEN
                     FGVIEQPTLKFELFYFDIFTYGTSFVTLQVPREEEFSPLKNKEGKDSVATATEDLRRM
                     GLI"
     gene            14033..14716
                     /locus_tag="PPY92_000551"
     CDS             14033..14716
                     /locus_tag="PPY92_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDJ2624575.1"
                     /translation="MSKSTKKSNDTIVETFKDIIPLSFGEEIGNAASHGVAALLTLLV
                     LPYAAVHSYIAHGTLASVSMSIYVISIFMMFISSTIYHSMQNETPHKYILRIIDHSMI
                     YVAISGTYTPILLTVVGGWIGWTVFILLWGTTLWGILYKSIAVKVNQKLSLIVYLVMG
                     WVGIILLPIIFIRTSWIFILFIFLGGVSYTIGAWFYAQKNRPYFHMIWHIFIVIASLL
                     HLIAILYFM"
     gene            14893..16236
                     /locus_tag="PPY92_000552"
     CDS             14893..16236
                     /locus_tag="PPY92_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDJ2624576.1"
                     /translation="MRLKSIITVIALILIMFMSAIESSIISLALPTIKQDLNAGNLIS
                     LIFTAYFIALVIANPIVGELLSRFKIIYVAIAGLLLFSIGSFMCGLSTNFTMLIISRV
                     IQGFGSGVLMSLSQIVPKLAFEIPLRYKIMGIVGSVWGISSIIGPLLGGGILEFATWH
                     WLFYINIPIAIIAIILVIWTFHFPEEETVAKSKFDTKGLTLFYVFIGLIMFALLNQQL
                     LLLNFLSFILAIVVAMCLFKVEKHVSSPFLPVVEFNRSITLVFITDLLTAICLMGFNL
                     YIPVYLQEQLGLSPLQSGLVIFPLSVAWITLNFNLHRIEAKLSRKVIYLLSFTLLLVS
                     SIIISFGIKLPVLIAFVLILAGLSFGYIYTKDSVIVQEETSPLQMKKMMSFYGLTKNL
                     GASIGSTIMGYLYAIQSGIFGPNLHNVLSAVAVISIGLIVLWVVFFKEQSSQSKE"
     gene            16335..16808
                     /gene="sepA"
                     /locus_tag="PPY92_000553"
     CDS             16335..16808
                     /gene="sepA"
                     /locus_tag="PPY92_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter SepA"
                     /protein_id="HDJ2624577.1"
                     /translation="MIVNYLKHKFYNLLTTMIVLFIFVLSGAIFLTFLGFGLYGLSRI
                     LIYFRLGDFTYNRSMYDNLLYYGSYIIFGYFIIFAVEHLMDYFRKMLPENAYFRGATF
                     HLISYTVATTLFYFIIHLNYVYINIDFWVIMVIIGFLYVCKLQFYPESKNLNNRK"
     gene            17131..18573
                     /gene="lmrS"
                     /locus_tag="PPY92_000554"
     CDS             17131..18573
                     /gene="lmrS"
                     /locus_tag="PPY92_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter LmrS"
                     /protein_id="HDJ2624578.1"
                     /translation="MAKVELTTRRRNFIVAVMLISAFVAILNQTLLNTALPSIMRELN
                     INESTSQWLVTGFMLVNGVMIPLTAYLMDRIKTRPLYLAAMGTFLLGSIVAALAPNFG
                     VLMLARVIQAMGAGVLMPLMQFTLFTLFSKEHRGFAMGLAGLVIQFAPAIGPTVTGLI
                     IDQASWRVPFIIIVGIALVAFVFGLVSISSYNEVKYTKLDKRSVMYSTIGFGLMLYAF
                     SSAGDLGFTSPIVIGALIISMVIIYLFIRRQFNITNVLLNLRVFKNRTFALCTISSMI
                     IMMSMVGPALLIPLYVQNSLSLSALLSGLVIMPGAIINGIMSVFTGKFYDKYGPRPLI
                     YTGFTILTITTIMLCFLHTDTSYTYLIVVYAIRMFSVSLLMMPINTTGINSLRNEEIS
                     HGTAIMNFGRVMAGSLGTALMVTLMSFGAKIFLSTSPSHLTATEIKQQSIAIGVDISF
                     AFVAVLVMAAYVIALFIREPKEIESNRRKF"
     gene            18721..19785
                     /locus_tag="PPY92_000555"
     CDS             18721..19785
                     /locus_tag="PPY92_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HDJ2624579.1"
                     /translation="MLTVDQVKELVGEIKDPIIDVPLKETEGIVEVSIKEEKEHVSVK
                     LAMAQLGGAPQLDLQMAVVNALKENGAKTVGIRFETLPEEKVNQFKPKEENKPKTIEG
                     LLSQNNPVEFIAIASGKGGVGKSTVAVNLAVALAREGKKVGLVDADIYGFSVPDMMGI
                     DEKPGIKGKEVIPVERHGVKVISMAFFVEENAPVIWRGPMLGKMLTNFFTEVKWGDIE
                     YLILDLPPGTGDVALDVHTMLPSSKEIIVTTPHPTAAFVAARAGAMAKHTDHSILGVI
                     ENMSYFESKETGNKEYVFGKGGGTKLADELNTQLLGELPLEQPSWNPKDFAPSIYQSD
                     DRLGKIYSSIAQKVIAATNK"
     gene            19874..20058
                     /locus_tag="PPY92_000556"
                     /pseudo
     CDS             19874..20058
                     /locus_tag="PPY92_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500888.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         6723 a         2719 c         3691 g         6999 t
ORIGIN      
        1 agaatctatt atgactgtag ataacaataa agcaaaacaa gcatacgaca atcaaactgg
       61 tgttaacgaa aaagaaagag aagagcgtca aaaacaacaa gaacaaaatc aagagcctca
      121 attcaaaaat aaattaactt tctctgatga agttgttgaa aaaattgctg gtatcgctgc
      181 acgtgaagtt aaaggtatct tagacatgaa aggtggctta actgatacat tcactaatgc
      241 attctcaagt ggaaataacg ttactcaagg tgtatctgtt gaagttggtg aaaaacaagc
      301 tgctgtagac ttaaaagtaa ttttagaata tggtgaatca gcaccaaaaa tcttccgtaa
      361 agtaactgaa ttagttaaag aacaagttaa atatattact ggtttagatg ttgttgaagt
      421 taacatgcaa gttgatgatg taatgactca aaaagaatgg aaacaaaaac acgaaaaaaa
      481 taatgaaaac aataaccaag aaagacaagg tttacaataa gttattcgat aaaaaagctc
      541 ggcacaccat tttagtgccg agcttttttg tgatttttaa taatttgaat atggaaaatg
      601 tattattctc tcatttgtat agattgtatt taataagtta atgtaatcct tgagctcacg
      661 attaataaaa ttctataatt ttaattattt tctcgataca aaggattatt aactttaata
      721 taagtataat gatgtgcctc atcttcaaga cgcattgttg taacacactt ataatctata
      781 aatggcgcga acatggtatc tttaatttca tttatgcgat tctcatttac ttgattagat
      841 ttgtgtgtcg aaagtacaag ttgatcaaaa atgttatcta gtacatcacg aacgatatac
      901 cacatatgtc tttctaagtt tgaatcattt gatgctttag agattgtaag aattaattcg
      961 cctaaatggt tttgaacggt agaataaaag gctttgttaa acacagacgt ttttgaatca
     1021 gtaagaattc ttgatttttc atggaaatga gatgtactgt atcccatttc atttagttgt
     1081 gctttatcaa ttcttaaacc ttcaaagtct ctaatataca tcgtgtccaa tagaccatct
     1141 ttccgaaatg ttgcaatagc attttgcaag tgagcctcta atgcaatgcc atatttagta
     1201 actagtggga ttacgagacc aagcaatgct ttactataag tttcaatcca cgatttcgcc
     1261 gatgattcaa aatcagacaa tgatgcagct gattgataac gtttaatcaa tgtcacgata
     1321 ggtgattcat tgttaaatgg gtaggttgca actaagcttg aaggaatcat tggtgtgact
     1381 tcttgtggaa tcatttggta tatgtttttt ctaaataatg tacctaattg ttcacttcgt
     1441 tcagtttgat agtctacttc gtcttgctca ttataaaaat gaataccagc aacttcatca
     1501 ataattgttg atgcatagga cttaaatatg acatctttct ccaaaatatc atttaaaata
     1561 cgtgtcatta gtggaccatt gtgcgtcgtt tgttctgata atgtacgaat ttcacctgta
     1621 atatgaacgt ttgtcgacaa tttgatgtgt ggcgacatag ctgggtattt aggaactaat
     1681 gttctgaaag ataaaccggc ataataatcc aacgtatgtt ttgcttcaat gattaattct
     1741 ttatctactt ctgcttgata atcagaatgt aatacgtcat ctaattgcca tggatgtaca
     1801 atcataatgt gataatcatt aaagttaaag tttggcgtaa attcattttc taattgttta
     1861 attaagtccg gaaatagctg atgaacagtt gtatcataat ctttagacag tgacatagta
     1921 cgacttaatt tactgtgaat cagtactatt ttcaacttaa taggttgatt aaattctgaa
     1981 gaatatagga atgtttgtaa tgcatttaaa cctttacgta atttagcccc aggatgtagc
     2041 ggatgacctt caataacggc ttgctctgaa cgcaagtaac tatcttcgct attttcgata
     2101 atattaaata aaggtgcaga atcatgttgc attgacagtg cttgatagct aattgcaaat
     2161 gtcatattag ttgcactgtt tattaaatct tgctgaaatt gatcactagc agcatttttt
     2221 aaatctggtg cttcaattaa aatacactca agaatttcat ttggatggtg tactcgtgta
     2281 atcgtatttg taatgtcgtc tttaatgtag aaagggcctt caacatcaat tcgatcaaag
     2341 gcgtgttctc cagtgatagg agcatataat gtttgcttag cttgtgggaa ctggatttct
     2401 agtatatgag tcgttgagat atctaacata atcaaatcac gactcaatat tttcttactt
     2461 tgagtgcgcg ctttaactaa gttttcgcga tgcattgatg tgactaatct ctgagtgact
     2521 ttatctcttc cttgtaaaat catctcttta aaaatattag cccaatcgct attatgttgt
     2581 tgtaaaaata aatatgtttc ttgttcttct ttgttaaatt ttaatgtctg ttctttaaaa
     2641 attaagttca agttcataat tcacctctat gaaatatttt acaaaagcaa gatagatttg
     2701 tataatccat attaatgata atgattctta ttatcaacag aatgcgggtg taagttttat
     2761 gacaaaatat ttttttagca gttcttttct actatttcta ggtaattgga ttggacaaat
     2821 agggctaaat tggtttgtac ttaccactta tcataacgca gtttatctgg ggattgtcaa
     2881 tttttgcaga cttgtaccaa tattattact aagtgtgtgg gcaggggcaa ttgccgataa
     2941 atatgataaa gggcgattgc tgagaattac aatttcatca tcatttttag taactgcaat
     3001 tttatgtgtg ctcacgtata gtttcactgc aattccaatt agcgtcatta ttatatatgc
     3061 gacattaaga gggattttaa gtgcggttga aacaccttta agacaagcaa tcttaccaga
     3121 tttatcagat aaaatatcta ctacacaagc tgtatcattt cattcattca tcattaatat
     3181 ttgtcgttca atagggcctg ccattgctgg tgtcatatta gcagtctatc atgcgccaac
     3241 aacatttctt gcacaggcaa tttgttattt tatcgcagct ttattatgct taccattaca
     3301 ttttaaagta actaaaatac ctgaagatgc aacaagatac atgccgttaa aagttattat
     3361 agattacttc aaattacata tggaaggtcg acaaatattt ataacatcat tattgattat
     3421 ggcgacaggt ttttcatata cgacactttt accagttttg acaaacaaag tatttccggg
     3481 gaaatctgaa atatttggta tcgctatgac gatgtgtgcc attggtggta ttattgcaac
     3541 gctagtttta cctaaagtac ttaaatatat tggtatggta aatatgtatt atttaagttc
     3601 acttttattt ggcattgctt tgttaggtgt ggtatttcac aatattgtca tcatgttcat
     3661 ttgtattaca ttgattgggt tatttagtca atgggcacgt acgacaaatc gcgtttattt
     3721 tcaaaataat gttaaagatt atgaacgtgg taaagtactg agtattatta tgatggatag
     3781 aggtatgatt ccattgggaa gtctgttaat gagtatatgt gcagatgtgt ttggcattgt
     3841 tagaactttt tcaataatgg gaataagtac tatatgcatt acaatggtat tctattttat
     3901 aaatagaaag ttgaagttaa agttggagga aagtaatcat ggtatatctt gaatgggcaa
     3961 aggcagatag aaatattcaa tatcgtgtaa ttaacgccat tattaaagaa cgtatttacc
     4021 ccgagcaaac atttatttcg caaaaaggat ctttaattga aattcagtat catatgcatg
     4081 tgttgactat tgaagttgtt agaaaaagtg cattagaacg ctatgagttt acaggtgata
     4141 ttacttattt aaataaaggt gaaacgtcat taattataac tttagaaggt ttgttagatg
     4201 tgttgaatca tgactttgat atccctattt cagagcgact acgcgaagag ttaatataca
     4261 gtcgagatag tttagttgaa acatataagc aaatgtctca cagacaaaca ttaataagtc
     4321 aaagttttaa attttcaaga ttaccacaag atattaactt tttttcatgg ttacaacatg
     4381 taaaagatag tgataagaca gatgatttaa cttattctga gagcttggta ccagaggggc
     4441 atccaacaca ccctttaacc aaaacgaaat tgcccttaac tatggaagaa gtacgagcat
     4501 atgcacctga gtttgaaaaa gaaatccctt tgcaaattat gatgattgaa aaagatcatg
     4561 ttgtgtgcac agctatggat ggtaatgatc aatttattat tgatgaaata attcccgaat
     4621 actacaatca gattcgtgtg tttttaaaga gtttaggttt gaaaagtgaa gactatagag
     4681 cgattttagt acatccttgg caatatgatc atacgatagg gaaatatttt gaagcatgga
     4741 ttgctaaaaa aatattaatt ccaacgccgt ttacaatact ttcaaaagca actttatcat
     4801 ttaggacgat gtctttaatt gataaaccat accatgttaa gttgcccgtc gatgcacaag
     4861 caacaagtgc cgttagaaca gtctcaactg tgactactgt agatggacca aagttaagtt
     4921 atgctttaca aaacatgttg aatcaatatc caggatttaa agttgctatg gaaccgttcg
     4981 gtgaatatgc aaatgttgat aaagataggg cacgtcagct tgcttgtatt ataagacaaa
     5041 agcctgaaat tgatggaaaa ggtgcaacag tagttagtgc aagtctagtt aataaaaatc
     5101 caatagatca aaaagttatc gtggatagtt acttagagtg gttaaatcaa ggaattacta
     5161 aagaaagtat tacgacattt attgaacgat acgctcaagc attaatcccg cctttaattg
     5221 cttttattca aaattatgga attgcattag aagcacacat gcaaaataca gtagtgaact
     5281 tggggccaca ttttgacatt caatttttag tgagagattt aggtggttct agaattgatt
     5341 tagaaacatt acaacatcgt gtatcagata ttaaaattac aaatgatagt ttaatagctg
     5401 attctataga tgcagtgatt gcaaaattcc aacatgctgt tattcaaaat caaatggcag
     5461 aattaatcca tcattttaat cagtatgatt gtgttgaaga aaccgaatta tttaacatag
     5521 tacagcaagt agtagcgcat gctattaacc caacactacc acatgcaaat gagttaaaag
     5581 atattttgtt tggaccaaca attactgtca aagcgttgtt aaatatgaga atggaaaata
     5641 aagtaaagca atatttaaat attgagttag ataatccgat aaaaaaagag gtgtagtact
     5701 acatggcaca cgttaacata aatatatcga agattaaata taacgccaaa gtacttcaaa
     5761 cagtttttca aagtaaaaat atacaattca caccagtaat taagtgtata gctggtgacc
     5821 gtacaattgt agaaagctta aaagcgttag gtatcaatca tgttgcagaa tccagattgg
     5881 ataacataat tagtattgca gatcaggatt taacatatac gttattgcgt acgcctgcca
     5941 aaaaagagat ttcagatatg atagaaaaag ttgatatgag tatccaaaca gaattatcta
     6001 ccatacatca aataaatgaa gtagcagaag ttttaggaaa aaaacataag attttattaa
     6061 tggtagattg gaaagatggc cgtgaaggtg ttctgactta tgatgtttta gattatatta
     6121 aagagattat tcacttgaaa aatatccatt ttgtaggttt agcattcaat tttatgtgtt
     6181 ttaaatctga tgcgccaagt gatgatgata tctttatgat taatcgattc gtctcagctg
     6241 ttgaaagaga aataggatat cgattgaaaa ttatttcagg tggtaattcg agcatgttac
     6301 cacaactttt atataatgat ttgggtaaaa taaatgaatt gcgaataggt gaaacgttgt
     6361 ttagaggtgt tgatacaacg acaaatcaag cgatcgctat gttatatcaa gatgcgatta
     6421 cattggaggc tgagatttta gaaattaaac ctcgagttaa tacacaaacg catgaatcat
     6481 tcttacaagc tattgtagat attggttatt tagatacaaa agtcgataat atttcaccta
     6541 tggatcagca tatcaatatt ttaggcgctt cgagtgatca tttaatgctt gatttaaatg
     6601 gtcaaggaca ctatcaagtt ggagatcata tatctttttc attaaattat gaagcacttt
     6661 ctcattcgat gtatatgaaa aatttacata aggtatatat tgatgattca aagattgata
     6721 cgctacttca aaattttgat gtgaaatctc ctgctatggt aaaccaatat taatatattt
     6781 atcaataatt ctaagttgac aagttatact acatatcgtt tgcacggttg tatcataatt
     6841 gttcaactta gattttttgt atttgttgat ttatcaaatt aagtgcaaca gttcgtcaca
     6901 taaaattgca acagataata tcagctgaat tacagggata acggtcatgc taaatggtgt
     6961 caattgtatt aatgcaaaga tgatatagca atgatacatt atcagtattt tgtctaagga
     7021 aatgtgctaa ttgtagtcat aattattaga ggaaatatat agtcatacat tttagaaata
     7081 taaaaaagat tgaacgctac ttgacaatga taattgttat caataaaata ataaatgaag
     7141 ttatacatat taaggagtgg aacgatgaga ggtctaaaaa cttttagtat attgggatta
     7201 atagttgcct tacttttagt tgcagcttgt ggtaatacgg ataattcaag taaaaaagaa
     7261 tcatcaacta aagatactat ttcggtaaaa gatgaaaatg gtacagtaaa agtacctaaa
     7321 gatgcaaaac gtatcgttgt attagagtac tcatttgcag atgcattagc agcattagac
     7381 gttaaaccag ttggtattgc tgatgatggt aagaaaaaac gtatcattaa accagttaga
     7441 gaaaaaattg gggattatac ttctgtaggt acacgtaaac agccaaactt agaggaaatt
     7501 agtaaattaa aaccggattt aattatcgct gatagcagta gacataaagg tattaataaa
     7561 gaattaaaca aaattgcacc aacattatca ttaaagagtt ttgatggaga ctacaaacaa
     7621 aatattaatt cgttcaaaac aattgctaaa gctttaaata aagaaaaaga aggcgaaaaa
     7681 cgtcttgctg agcatgataa attaatcaat aagtataaag atgaaattaa atttgataga
     7741 aatcaaaaag tgcttccagc agtagttgct aaagctggtt tattagcaca tccaaactat
     7801 tcatatgttg gacaattttt aaacgaacta ggatttaaaa atgcattaag tgacgatgta
     7861 acaaaaggtt taagtaaata tttgaaagga ccttacttac aattagacac tgaacattta
     7921 gctgatttaa atccagagcg tatgatcatt atgacagatc atgctaaaaa agattctgct
     7981 gaattcaaga agttacaaga agatgcaaca tggaaaaagt tgaatgcagt taaaaataat
     8041 cgcgtggata ttgttgaccg tgatgtttgg gcaagatctc gtggcttaat ttcttctgaa
     8101 gaaatggcta aagaacttgt tgaattatca aaaaaagaac aaaagtaagg tggaagtaaa
     8161 tggctataaa agaaataagt agccaatctg ccatagatca taaaagaaaa agacgcacaa
     8221 cactcacgta tatagtgagt ttgtgctttc tttttatttg tatatattta aatatggcga
     8281 ttggttcttc gaaaattaat tttagcgata tcattcacta tgttactggt catacagata
     8341 cgaaagcaac gtttttattg cataatgtac gtatgccaag gatgattgca gggttattta
     8401 ttggcggtgc attagcggta tctggtttgt taatgcaagc aatgacacgc aatccgctag
     8461 catcacctaa aatttttggt gtcagttctg gtgcatcgtt tttcattgta tttgtgacga
     8521 ttatcattcc atcattagaa tattatgcat tatatttagg tgtgataggt gcatttatag
     8581 gtggtttaac tgtttataca ctttcaggtg ctacgaaagg tatgacaccc attaaattag
     8641 cattggctgg tatggccatc catttgtttt ttagtagtat gactgaaggt attattattt
     8701 tgaatgagaa ttcaaacgaa caagtgatgt tttggcttgt cggttcatta tccagtatga
     8761 agtgggatga aattttaacc attttaccat ggattattgg tgctttaatt gtaacgattt
     8821 ttattggacg tcaactaacg attatggaac taggcgatga cattgctaag ggattgggtc
     8881 aaaatataaa taaagttaga attatcattg ggctattagt cattattctt acaggtatgt
     8941 cggtgtcagt ggcaggacca attggatttg ttggcttaat cgtaccgcat atagtcaaac
     9001 gctatgtgag taagaattat ttagtcatga ttccattaac atttattata ggtgcagatt
     9061 tgttactact atcagatgta ttaagtcgtt taatcactta tccttacgaa tcacctgtag
     9121 gtattgtgac atcattcgta ggtgcacttt acttcttatt tataacgatt aaaggggtga
     9181 aaagaatatg atgacaacga aaattatacg tcgttatagt ttaatcacaa tccttttaat
     9241 tgtgagtata ttcataagtt tatgtgtagg gtctgtcatg attcatccga ttgatgctat
     9301 taagggaatc tttacacaag atgattttat tttaaatgag tatcgtatac ctagaacatt
     9361 acttggtatt attatcggta gtagcttggc tatatcaggt gcaattatac aagctgtaat
     9421 aagaaacccg ttagcctcac ctgatgttat aggtatttct aaaggtgcca gtttagctgc
     9481 tgtaattatt attatgatct ttccaacagc accattgttt gttttaccta taggttcatt
     9541 cttaggtgcc tttgcagtaa gtttattttt atcattttta atttcaaaat atgatgttaa
     9601 aggttcaaag cttgccttaa ttggtttagc aataggcgca atatgtacgg cgattgtgca
     9661 gttcttatta atcagaaatc cactggaagc aaacaaagcg cttgtttggt tgacaggtag
     9721 tctatatgga catgatatgt cgaatattta tactatatta ccttggtttt taataacgat
     9781 accaatcatc attttattga gttatcaatt agatgtattg aatctaggtg atgctgttgc
     9841 aaccgcactc ggtttaaaag ttaaaactat taaaatgcta ttgctaattt tagcagtaat
     9901 attagcagga tccgcaattt cagttgtcgg tggtattagc tttttaggat taattgcacc
     9961 tcatattgcg agacaattaa taggaaataa aacattacac gttattatca tgtcggggtt
    10021 aattggtgct atattgttaa catttggaga tggtttagcc cgtggcattc atccgccact
    10081 ggatattcct gtaggggtta tcattgcaat tatcggtgca ccatacttct taatattatt
    10141 acgaagaatg aaataactaa catatgatta gagtttaaaa aaggttaagt acgaaatcag
    10201 taattgtact taaccttttt tgaattcatc ttgaaaaatt agaagtaata gcgtatattt
    10261 aataactgta attattcatt attttgcata cataaacatt ttgaaagata gaatgataag
    10321 gaatatgttt gttaactgaa tagaattctg tatatactta aaacgaatac atagagaaat
    10381 ggattgattt tatggatagc ttttcaagga aagagatagt tattgggaga cttaagttta
    10441 ttacgatgtc tttaattggt atcttattat ttctagtacc aattccagtc gaacaagatg
    10501 gacagaagca aacaacgttg cccgtagcat ttttagcagg cgtgcttaaa gatgtattag
    10561 gtggcgtaat gccattttta attgtgacga taattacttt atcgggtatc ataactctga
    10621 tttgttcaac tattttaaaa gataaattga aacctgacgg gctaatgaac aatgctttta
    10681 acgttagaat tggttggtta attttacgaa tattagcagt tgtttttgca tggatgacat
    10741 ttttacgaat tggttcaaag gttatttact cagatgaaac gggtggatta ttgttctcaa
    10801 gtttattgcc aacattagtg gcagtattct tatttgcagc tctattttta ccgctattaa
    10861 tggaatacgg tttgttagag atgttgggtc ctatttttag accggttatg agacctttat
    10921 ttacattgcc aggacgttcg acggtagata atttagcatc atttattgga gacggaactg
    10981 ttggtgtctt aattacaagt agacaatatg gtgaaggata ttattctcgt cgtgaggcaa
    11041 cagttatttc aacgacattt agtgtagttt ctatcacctt tgcaattgta gttgctgaaa
    11101 cagtgcacat gcaaaatcaa ttttttgctt tctatttatc agtgatagta tcctgcctcg
    11161 ttgcagcagt cattatgcca agaatttggc ctttaaataa aattcctgat gagtatgcta
    11221 aggaggtacc tgaaagtgcg cgtactgagg cattgccaga aggaaagacg gcattgagac
    11281 atggctttga tactgcaaca gaagttggta ttaaagcacc gggcgttatt gactttttta
    11341 agtcaggatt aaaaacagtc attgatatgt ggtttgttat tttgcctgta gtgatgagta
    11401 ttggtactat tgctaccatt attgcgaact acacgccctt ttttgtcata ttgggtaaac
    11461 cttttgttcc atttttagag ttaatgcaaa tacctgaagc ggcacaggct tctcaaacga
    11521 ttattatcgg ttttgccgat atgtttttac cttctatatt aattgaaggt gtacaaaatg
    11581 atataactcg ttttgttatt ggggcactta gtatatcgca gttaatatac ttgtctgaag
    11641 taggtggcgt catacttggt tcgaagattc ctgtaagcat cggcaaattg tttatgattt
    11701 tcttgattag aacaatcatt acactaccaa ttatttcttt gatggcacat cttttgttat
    11761 aaatatatgt gaaaaaggac aactgttaaa attatgagaa cagttgtcct tttgtatttt
    11821 tttatacaat ttatttagtt aattcattgt aaactttttg atcattttga gtataaataa
    11881 tgtattcgtg gtcttcagta tcgataataa cgcgattcgt tttaccaaac gttgagccga
    11941 ttctagatac atctttgctg tctaatttag gaactgcatg aatatcttgg tcttgtgtaa
    12001 tatttttaat ttcacttgta ggaattttga tatcagcaac tctccattga atacgcactt
    12061 cattattatc tttctttact gtcattgcca ttattcaaac acatccttta aatattttgt
    12121 aaatacatca taaacgattt tgtaatcgtt ttcaagagtt atgtgatact tttttaaatt
    12181 gtgtcatact aatgaatttt aacccgaaaa tatgttcgtg tttttgttag ggatgtggta
    12241 aaatgtgagt attaaaggag aacaaatatg acaggtaaaa cacatgcttc atgtggcatg
    12301 ctcgttggag cgctaacaac acaatatttt caaacggata ttttttcgtc ggttacggtt
    12361 atcattctag caactctagc tagcttatta cccgatatat gtcacactca aagtaagatt
    12421 ggcagaaggt ttaaggtaat tagttttttt gtgagattga tctttggaca tcgaaccttt
    12481 acgcattcaa tattatttat cgcaatcata gcatttttat tgcaaattat tcagacgcct
    12541 aattattata tggcagcaat tataattggg ttagtgtcac atgttatact agatatgata
    12601 acaccaagag gcgtgaaatt attttatccg ttaccgttta atgtgaagac accgattcaa
    12661 tttaaaacag gtggacttgt agacttatct ttagctacag cgttaatggt cggaacagtt
    12721 tatgttttat ttcagccttt catcaatgat ataataagca actggaatac taagttttta
    12781 tagaaaataa attattaaag gttacaaact cagcaggagg cataaaatgc tagataaaaa
    12841 tcaattagct aaatataaac aagatcattt gtgtgaatat gaaaaaataa tgagtaacaa
    12901 tgaaaaagaa gcacttgaag agaaggtagc gtcattagat ttagatttta ttgccaaatt
    12961 atataacgat ttatacatca ataagaaaac aatagatgat gtatctgcag tttctgaagt
    13021 gaaatatgat attaaatcac aaatgagtga tgatgagatt aagcgtttag aggaacaagg
    13081 acttcaagct attaaagaag gacaatttgc agtacttttg atggcaggtg gtcaaggaac
    13141 aagacttggt tacaagggcc ctaaaggttc gtttgaaatt gaaggtgtaa gtttatttga
    13201 acttcaagct aaccaattaa aaacattgaa tcatcaatct ggacatacga ttcaatggta
    13261 tattatgaca agtgatatca atcatgaaga aactttagct tatttcgaag cacatagtta
    13321 ttttggatat gatcaagaag caatccattt ctttaaacaa gataatattg tggcgcttag
    13381 tgaagaaggg aagttaattt taaatcaaca gggtcgtatt atggaaacgc cgaatggtaa
    13441 tggtggcgtg tttaaatctt tagacaaggc aggatactta gaagaaatgt ctaataatgg
    13501 cgttaagtat attttcttga ataatatcga caatgtttta gtaaaagtgt tggatccact
    13561 atttgcagga tttacggtag aacatgatta cgatattact tctaaaacga ttcaaccaaa
    13621 accaggagaa agtgttggac gtttagttaa tgtcgattgt aaagatacag tactggagta
    13681 ttcagaatta gatccagaag ttgcaaatca atttaacaat gctaatatcg gtattcatgc
    13741 atttaaactt ggttttattt taaacgctgt aaatcgtgag ttaccttatc atttagctat
    13801 taagaatttg aaacaattag atgagaactt tggggttatt gaacaaccaa ctttaaaatt
    13861 cgaattgttc tattttgaca tatttactta tggaactagt tttgtcacgt tacaagttcc
    13921 tagagaagaa gaattttcac ctcttaaaaa taaagaaggt aaagatagtg ttgcaactgc
    13981 aacagaagat ttacgtagaa tgggtttaat ttaatgatag gcggtgacta tgatgtccaa
    14041 atcaactaaa aaaagtaatg acacaatcgt agaaacattt aaggatatca tccctttatc
    14101 atttggagaa gaaattggta atgcggcatc tcatggtgtt gcagctttgt taacattatt
    14161 agtgttaccc tatgcggctg tgcacagtta tattgcacat ggtacgcttg cgtctgtgag
    14221 tatgtctatt tatgttattt ctatctttat gatgtttatt tcatctacta tttatcattc
    14281 tatgcaaaat gaaacacctc ataaatatat tttaaggatt atcgatcaca gtatgattta
    14341 cgtagcaatt tctggtacat atacgcctat tttgttaaca gttgtaggtg gttggatagg
    14401 ttggacagta tttattttat tatggggaac gacactttgg gggattttat ataaatccat
    14461 agctgtcaaa gttaaccaaa agttaagctt aattgtctat ttagtgatgg gctgggtagg
    14521 tattatcttg ttaccaatta tttttattcg cacatcatgg atattcatat tattcatttt
    14581 cttaggtggt gtgtcttata caattggtgc atggttttat gcacaaaaga atagaccata
    14641 ttttcatatg atttggcata tttttattgt aatagcatca ctattgcatt taattgcgat
    14701 cttatatttt atgtagtcgt ttagaaaagc tgggggaaac tccgcttttt ttattacata
    14761 aatcattcta ataggggatt gtattgtaat agaattatcg ttaagatttg aaagtcaaaa
    14821 gtgtttatac atatgaaaac caattatttg taaataaaat cttataaata aatatataga
    14881 aggaggtatt tcatgcgatt aaagtcaatc ataactgtta tagctttaat attaattatg
    14941 tttatgtcag cgattgaatc atctattatt tcgcttgcat tacctacaat taaacaagat
    15001 ttaaatgcag gaaatttaat ttcattaatt tttacagcgt attttatcgc attagtaatt
    15061 gccaatccta tagtgggaga attactttcg agatttaaaa ttatttatgt ggcaattgca
    15121 ggattacttt tatttagtat aggtagtttt atgtgtggtt taagtactaa ttttactatg
    15181 ctaattatat cacgcgtgat acaaggtttt ggatcaggtg tattgatgtc attatcgcaa
    15241 attgttccaa agttagcatt tgaaattccg ttacgatata aaattatggg catagttggc
    15301 agtgtttggg gtatttcgag tatcattgga ccgcttctag gtggtggtat attagaattt
    15361 gcaacatggc attggttatt ctacataaat ataccgattg cgatcattgc cattatatta
    15421 gttatatgga catttcattt tccagaggaa gaaacagttg ctaaatcaaa atttgatact
    15481 aaagggctca cgctatttta tgtatttatt ggattgatta tgtttgcttt attaaaccaa
    15541 cagctattac ttttaaattt cttgagtttt atcttagcta tcgttgtggc aatgtgttta
    15601 tttaaagtag aaaaacatgt ttcttcacca tttttacctg tggttgaatt taatcgttcg
    15661 attactttag tttttataac ggaccttcta acagctattt gtttaatggg attcaattta
    15721 tatattccag tctaccttca agaacaacta ggattatctc cattgcaaag tggattggtt
    15781 atttttcctt tatctgtagc ttggattaca ttgaatttta atttacatcg aattgaagca
    15841 aaactatcaa ggaaagttat ttacttacta tcatttacat tgttactagt aagtagtatt
    15901 atcatttcat ttggtattaa attgccggta cttatagcat ttgtgttaat tttggcagga
    15961 ttaagttttg gatatattta tacgaaagat agtgtaattg tccaagagga aactagccca
    16021 ttacaaatga agaaaatgat gtcattttat ggattaacta aaaatcttgg ggcatcaata
    16081 ggttcaacaa taatgggata tctttatgcg atacaatcag gaatctttgg tccaaactta
    16141 cacaatgtgt taagtgctgt tgctgtaatt agcattgggc ttattgtttt atgggtcgta
    16201 ttttttaaag aacagtcatc tcaatcaaaa gaatagaatt atgattaaaa ttgatgtagt
    16261 gtaattaaag taagaaataa taaatttcct agatactaga taaatcgttc aatattttat
    16321 taataaggag gccaatgatt gtgaattatc ttaaacataa gttttataac ttgttaacta
    16381 caatgattgt tctctttatt tttgtacttt ctggtgcgat ttttttaaca tttctagggt
    16441 ttggtttata tggattaagt agaatactta tttattttag gttgggtgac tttacatata
    16501 atagaagtat gtacgataac ctattatatt atggcagtta tataatcttt gggtacttca
    16561 tcatatttgc agtcgagcat ttaatggatt attttagaaa aatgttgcct gaaaatgcat
    16621 attttagagg cgcgactttt catttgattt cttacacagt tgcaacaacg ttattttatt
    16681 tcatcattca tttaaattat gtttatatca atatcgattt ttgggtcatc atggttatta
    16741 tagggttctt atatgtttgt aagcttcaat tttaccctga aagtaaaaat ttaaataata
    16801 gaaaatagat atctgaagtt gagggcacat atgtgttcca acttcttttt gttcaataga
    16861 agaatcgata atagtgagtc ttagtgctca atttgtggta aataagttaa ataaccattg
    16921 tatgagtctg agaaataaaa taaattatta ggccctacaa tgttatattg ttagtttttt
    16981 aagtacacat tagctgtgcc taatgtgtaa gaaccaataa tgatttatta tgtagtgctt
    17041 ctttatcatt tgagtactac tcccattatg ttaatggcta tatgtattaa agggacaagt
    17101 gtatatctgt tttaaaaagg aggaattatg atggctaaag ttgaattaac aacccggaga
    17161 cgaaacttca tagttgctgt tatgttgatt agtgcgtttg tagctatttt aaatcaaaca
    17221 ttattaaata cagcgttacc tagtataatg agagaattaa atattaatga aagtacatcg
    17281 caatggctag ttactgggtt tatgcttgtt aatggcgtca tgatacctct gacggcatat
    17341 ctaatggata gaattaaaac tagaccttta tacttagcgg cgatggggac atttttatta
    17401 ggttctattg ttgcagcctt agctccgaat tttggagttt taatgttagc acgtgtaatt
    17461 caagcgatgg gtgcaggcgt acttatgccc ttaatgcaat ttacgttatt tacattgttc
    17521 agtaaagaac atcgaggttt tgcaatggga ctagcaggtt tagtaattca atttgcacca
    17581 gcaataggcc ctacagttac aggattaatt attgatcaag cgagttggcg agtaccattt
    17641 attataattg taggaattgc tttagttgcc tttgttttcg gcttagtttc aatctcaagt
    17701 tacaatgaag tgaaatatac gaaattagat aagcgctcag taatgtattc aactattgga
    17761 ttcgggttaa tgctatacgc atttagtagt gcaggagatt taggatttac aagtccaata
    17821 gtaataggtg cgttgataat aagtatggtt attatctatt tatttatacg tagacaattt
    17881 aatattacta atgtactttt aaatttaagg gtttttaaaa atagaacatt tgcattatgt
    17941 acgattagtt caatgattat aatgatgtca atggttggac ctgcgctgct tataccgcta
    18001 tatgttcaaa atagtttatc tttatctgcc ttgttatcag gacttgttat catgcctggt
    18061 gcaataataa atggtattat gtcagttttt acaggtaaat tttatgataa gtatggtcct
    18121 agaccattga tttatactgg ttttacaatt ttaacaatta ctacaattat gttgtgtttc
    18181 ttgcatacag acacatctta tacgtattta atagtcgtat atgcaattag aatgttttca
    18241 gtttctttac tcatgatgcc gataaatact acaggaatta attctttgag aaatgaagaa
    18301 atctcacatg gcacggctat tatgaacttt ggtcgtgtaa tggctggttc actaggcaca
    18361 gctttaatgg ttacattaat gagttttggt gcaaaaatat ttttatctac atcgccatcg
    18421 catttaactg caactgaaat taaacagcaa tccattgcta taggggtgga tatctcattt
    18481 gcttttgtag ctgtgcttgt tatggcagct tatgtgatag cactttttat aagagaacct
    18541 aaagaaatag aaagtaatag aaggaaattt taaaataatt atagtagttg gtctatttaa
    18601 aataataggc taactgcttt ttttatttaa taaaaagttt tatactttta gtgatagact
    18661 aagcaaaaat tgttatttgc tatgatgtag atgtcttaaa atgattaagg gggatttgct
    18721 ttgttaacgg tagatcaagt gaaagaattg gtaggagaaa ttaaagatcc tattatagat
    18781 gtgcctttaa aagaaacaga aggtattgtt gaagtttcta ttaaggaaga aaaagaacat
    18841 gtgagtgtta aattagcaat ggcacaatta ggtggtgcac cgcaattaga tttacagatg
    18901 gctgttgtta atgcattaaa agaaaacggt gcgaaaacgg tcggtatacg atttgaaaca
    18961 ttgccggaag aaaaagtaaa tcaatttaaa ccaaaagaag aaaataaacc taaaacgata
    19021 gaaggcctat tatctcaaaa taatccagtc gaatttattg caatagcctc cggtaaaggt
    19081 ggtgtcggta aatctactgt tgcagtaaat ttagccgttg ccttagctcg tgaagggaaa
    19141 aaagtcggat tagtagatgc cgatatatat ggatttagtg taccagatat gatgggtatt
    19201 gatgaaaagc ctggaattaa agggaaggaa gtaattccag ttgaacgtca tggcgttaaa
    19261 gttatatcaa tggccttttt tgtggaagaa aatgcgccag ttatatggag agggccaatg
    19321 ttaggtaaaa tgttgacgaa tttctttaca gaagttaaat ggggagacat tgaatattta
    19381 atactcgatc ttccacctgg aacaggagat gtagctttag atgttcatac gatgttacct
    19441 tcaagtaagg aaattattgt aacgacacct catcctacag cagcatttgt tgcagctcgc
    19501 gcaggtgcga tggcaaaaca tacggatcat tctattcttg gcgtaattga aaacatgagt
    19561 tattttgaaa gtaaagagac gggtaataaa gaatatgtct ttggcaaagg tggcggtact
    19621 aagttagctg atgaacttaa tactcaatta cttggggaat tacctttaga gcaaccatct
    19681 tggaatccaa aagattttgc accgtcaata tatcaatcag atgatcgtct aggtaaaatt
    19741 tatagctcga ttgcacaaaa agttatagct gctacaaata aataggtata atatatgcta
    19801 aacgtacaaa attactttta ttttgtacgt ttttatgttg atttgttttg gaatgctggt
    19861 aaaataagtt cttgtgttaa gcaataaacg cgttgaaaaa gaagcgggtt gtgaacacaa
    19921 aaagttttaa aaaagttatt gactttaaat tccaaagttg ataatatgat aaagttgtca
    19981 aaaacgacgc agttgttttt aaagaagaat ttcaaaagtg taaaacttac cattgaaata
    20041 actataaaaa caatatagaa taaataaagt aatcgttcaa aaagttattg acttcaagaa
    20101 acaaatgaag tagaataaaa gagattgaat at
//
LOCUS       DANBWH010000022        20070 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.022, whole genome shotgun sequence.
ACCESSION   DANBWH010000022 DANBWH010000000
VERSION     DANBWH010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20070
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.022"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            25..837
                     /locus_tag="PPY92_000557"
     CDS             25..837
                     /locus_tag="PPY92_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014936986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDJ2624580.1"
                     /translation="MEYIKKIALYMSVLLLIIFIGGCGNMKEEQKKEANTNKTDSKEE
                     KIKKSFAKTLDMYPIKNLEELYDKEGYRDGEFEKGDKGMWTIYTDFAKSNKPGELSNE
                     GMVLYLDRNTRTAKGYYFVRTFYRKDKLPDRKNYKVEMKNNKIILLDKVEDPNLKKRI
                     ENFKFFGQYANLKELKNYSNGDVSINENVPSYDVKYKMSNKDENVKQLRSRYNIPTDK
                     SPVLKMHIDGNLKGSSVGDRKLEIDFSKRENSHLSVIDSLDYQPAKVDEDER"
     gene            827..2152
                     /locus_tag="PPY92_000558"
     CDS             827..2152
                     /locus_tag="PPY92_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001581953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624581.1"
                     /translation="MKDKYKIDPGIIKNNTEETTAISKISYEVENANLYGADSEDITR
                     QIEYLKAKKKFPSNLEYVDSYTDSLNGVTTSAFLNKDTGKVTLGMTGTNIQGEAFKKL
                     KEGEFSRQNVTNALETVKDGYADLKILYSPASDQNYRYANTQEFINKIKSKYDIDFIT
                     GHSLGGRDAVVLGMSNGIPNIVVYNPAPISINSLNPNSPDGKRLLELYKNYKGNITRF
                     VAENDALTENLKKYKHYVFFGNDKVFKNGKGHEMEGFLTEEEQKAIKKELKKLQGYAE
                     ENNKSFVKNSNNAISKLASIELLRANMMTTNGGGLSSSQQKVLESLTALTIAQSFSQL
                     IDDEINQIKKMYNEKKKEFGKNWEDAQKAGKAVGEDLSVNGVLNALDEGQVNESSMVR
                     EPEQMISAKERQLSTIGSSVSNYIMRVRLSINEIVDKDQVLASQIGGLL"
     gene            2149..2472
                     /locus_tag="PPY92_000559"
     CDS             2149..2472
                     /locus_tag="PPY92_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624582.1"
                     /translation="MMFNQINNKNELEESYESEKKRIENELHNLNELRHRTRKENERS
                     YDVFQYLKHEMNYSEDAQRKMTRNIEAYEQEINEIIRKQEWKLEEYKEDLKKSYEKQL
                     DKLSD"
     gene            2491..2805
                     /locus_tag="PPY92_000560"
     CDS             2491..2805
                     /locus_tag="PPY92_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624583.1"
                     /translation="MTLIEPDMNLRMPDISTTVETLNLISKMEAQKENIRSVIAPEHK
                     HKYKDIENGLKGEEKVLIEQMAQHCEAFKANFKGAAQGDWVKSAMSEIDSIKDDLKKI
                     NS"
     gene            complement(3094..3294)
                     /locus_tag="PPY92_000561"
     CDS             complement(3094..3294)
                     /locus_tag="PPY92_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624584.1"
                     /translation="MNMLFVILVLYVIGIAFILLSVFGSKTEGLSTKHTLYTIGSAII
                     TIAIFISIGYAIQYLTAALYGL"
     gene            3473..4675
                     /locus_tag="PPY92_000562"
     CDS             3473..4675
                     /locus_tag="PPY92_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDJ2624585.1"
                     /translation="MAKIPVTVLSGYLGSGKTTLLNHILQNREGRRIAVIVNDMSEVN
                     IDKDLVADGGGLSRTDEKLVELSNGCICCTLRDDLLKEVERLVKKGGIDQIVIESTGI
                     SEPVPVAQTFSYIDDELGIDLTAICRLDTMVTVVDANRFVHDINSEDLLMDRDQSVDE
                     TDERSIADLLIDQVEFCDVLIINKIDLISEEELAKLEKVLSALQPIAKIIKTTNSEVD
                     LKEVLNTQRFDFEKASESAGWIKELESGGHASHTPETEEYGISSFVYKRRLPFHAKRF
                     NDWLESMSNNVVRSKGIVWLAQYNHVACLLSQAGSSCNIHPVTYWVASMSEAQQTQIL
                     AERQDVAAEWDPEYGDRHTQFVIIGTELDEEKLTKELDACLVNAQEIDADWQQFEDPY
                     QWQIRPAR"
     gene            complement(5334..5396)
                     /locus_tag="PPY92_000563"
     CDS             complement(5334..5396)
                     /locus_tag="PPY92_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-4"
                     /protein_id="HDJ2624586.1"
                     /translation="MAIVGTIIKIIKAIIDIFAK"
     gene            complement(5460..5528)
                     /locus_tag="PPY92_000564"
     CDS             complement(5460..5528)
                     /locus_tag="PPY92_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-3"
                     /protein_id="HDJ2624587.1"
                     /translation="MEFVAKLFKFFKDLLGKFLGNN"
     gene            complement(5580..5645)
                     /locus_tag="PPY92_000565"
     CDS             complement(5580..5645)
                     /locus_tag="PPY92_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-2"
                     /protein_id="HDJ2624588.1"
                     /translation="MGIIAGIIKFIKGLIEKFTGK"
     gene            complement(5677..5742)
                     /locus_tag="PPY92_000566"
     CDS             complement(5677..5742)
                     /locus_tag="PPY92_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-1"
                     /protein_id="HDJ2624589.1"
                     /translation="MGIIAGIIKVIKSLIEQFTGK"
     gene            6263..7747
                     /locus_tag="PPY92_000567"
     CDS             6263..7747
                     /locus_tag="PPY92_000567"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit 5"
                     /protein_id="HDJ2624590.1"
                     /translation="MLSFQLLFSLFVIALIIALISGLLFLAPVMPMRYIKLHLYILVM
                     PVLFAVIGFFGIHGQHVLGPFKIDRLSWLLAGFVMALGFIIQKFSMRYLLGDHHYRHY
                     FPLFTAITSFASLAWMSEDLRLMALCWGMTLLCLTLLMNVNRFWKVPRESAKLSSMTF
                     LCGWLAFVGAIVTIYIATGEWRVPQHIVHPTWSLLTNVLLVLAVMIPAAQFPFHRWLI
                     ESVTAPTPVSAIMHAGIVNAGGVILTRFAPIFDNGFALSLLLILSSISVLLGSGISLV
                     QVDYKRQLVGSTMSQMGFMLVQCALGVYSAAIIHLILHGIFKATLFLQSGSIVKRFNI
                     PKQASAKDAYGWIVMGRVLAIIVAFLFWMSSDRSAYEVLSALILAWSLLVSWNQMVAF
                     SKGRMARLVGMILIAIVTFIYVITHNYFYDVLQNITTHATTPPTVSVIISVVILIFGS
                     LLSIWVARHRYSKGFAVLYVWLVNLGEARSKAIESHPNYLKKYL"
     gene            7760..10465
                     /locus_tag="PPY92_000568"
     CDS             7760..10465
                     /locus_tag="PPY92_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcC family protein"
                     /protein_id="HDJ2624591.1"
                     /translation="MTTQLNINSVIENAKRVITPLSPISIFAARNPWEGLEADTFEDV
                     AKWLRDVRDVDIFPNKALIESAVARGELDESVFNQLVTDMLLEHHYNIPQHYINLYID
                     NIKTLKDVPASYMNHSNVDVVADLLLEKSKRDMAESYHHYDVRPMSDAIIDEQGEPLS
                     EQVNRQMIKWTKLYIDQFLSSWTMPKREQSFYHAWLHLAQHDHSFTKAQRQVIKGLPN
                     DPEMTIESVLTYFSIDQEDYQAYVEGHLLALPGWAGMLYYRSQQHHFEQHLLTDYLAI
                     RLVVEQLLVGDEFKSVTKDCESRSENWFKQTVASLCYYSDMPSDVLLQHDVNEIQTFI
                     HFAATMNKNVFKNLWLIAWEMTYESQLKQKIKAGHESVAGALDVNQVNVSENDNANQP
                     HSVLLNDTQAVDENNSELNQVGTSTKAQIAFCIDVRSEPFRRHIEAAGPFETIGIAGF
                     FGLPIQKDAVDEQFKHDSLPVMVPPAYRIKEFADRYDMNVYRQQQQTMSSMFYTFKLM
                     KNNVMPSLLLPELSGPFLSLSTIVNSIMPRKSRASLQKIKQKWLKKPETKLTIDREFD
                     RTSDLPVGFTEQEQIDFALQALKLMDLTEAFAPFVVLAGHASHSHNNPHHASLECGAC
                     GGASSGFNAKLLAMICNRPNVRQGLKQSGVYIPETTVFAAAEHHTSTDTLAWVYVPDT
                     LSALALDAYESLNDAMPMISEQANRERLDKLPTIGRVNHPVEEAQRFASDWSEVRPEW
                     GLAKNASFIIGRRQLTKGIDLEGRTFLHNYDWRKDKDGKLLNTIISGPALVAQWINLQ
                     YYASTVAPHFYGSGNKATQTVTSGVGVMQGNASDLMYGLSWQSVMAADRTMYHSPIRL
                     LVVIQAPDYVVARLFANNEHFARKVSNHWLRLMSVNEEGRFKSWI"
     gene            10627..10989
                     /locus_tag="PPY92_000569"
     CDS             10627..10989
                     /locus_tag="PPY92_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HDJ2624592.1"
                     /translation="MKRTKGEIEAEISKAITQWEKDFLGRGSLSVKSDILRDMVIISL
                     QGILTPAEYRVCSTNEGLLNIKRTRSELVESGEQDLNDIILKITGIKVMSFHSDLSTV
                     TGERIIVFKLEDNLEKHI"
     gene            complement(11218..11634)
                     /locus_tag="PPY92_000570"
     CDS             complement(11218..11634)
                     /locus_tag="PPY92_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624593.1"
                     /translation="MLSIISMCHKFHFTDNIKIIKFILLCILQIKHCIIEITIFCYFQ
                     FSTIYIYYTEGVRRINRVIFVYIALIITLVRFFTPIHPSFSNLIYWIFVLYFIPIILC
                     VIGFKAEKLIATMVIIPNFLGILYRLYAYVTHILFM"
     gene            11672..12346
                     /locus_tag="PPY92_000571"
     CDS             11672..12346
                     /locus_tag="PPY92_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDJ2624594.1"
                     /translation="MIDKKLTSPKMTVPLFLIALIVFIGMFYGVVTNQEWLKNIDMGS
                     LTWFTDYFGEPQRQYVNNLFNYYMTFSAEIGDVKGVVLISIIVTIILFIKQRHLAVWF
                     VTYLVSGVIMNKLIKDTVLRPRPYNHLAVDTGFSFPSGHSNASTLLYFALMIIIISVA
                     AKTITKVLSALVMGILWLSILFCRLYFHAHYFSDVIGGTSLAIIWVALFLMVYPYFIN
                     HRRQRV"
     gene            12383..13117
                     /locus_tag="PPY92_000572"
     CDS             12383..13117
                     /locus_tag="PPY92_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HDJ2624595.1"
                     /translation="MRIKTPSPSYLKGTNGHAILLLHSFTGTNRDVKHLAAELNDQGF
                     SCYAPNYPGHGLLLKDFMTYNVDDWWEEVEKAYQFLVNEGYESISATGVSLGGLMTLK
                     LAQHYPLKRIAVMSAPKEKSDDGLIEHLVYYSQRMSNILNLDQQASSAQLAAIDDYEG
                     EITKFQHFIDDIMTNLNVIKIPANILFGGKDEPSYETSAHFIYEHLGSVDKELNGLKD
                     SHHLMTHGEGRDILEENVIRFFNALT"
     gene            13489..14826
                     /locus_tag="PPY92_000573"
     CDS             13489..14826
                     /locus_tag="PPY92_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HDJ2624596.1"
                     /translation="MKRQQSQWKSSTGFILASAGSAIGLGAMWKFPYMAGIYGGGAFL
                     AMFLIFTIFVGLPLLIMEFTVGKMGRTYTTQIYSKLTGKKWLNIIGWNGNLAVFILFG
                     FYSVIGGWIVIYIGQVLWQLVIFQRINHLQEMNFEAVISNPWLTVLGQGIFIFATMII
                     VMLGVEKGLEKASKVMMPLLFVFLIIIVIKSLTLDGALEGVKFILQPRVSEITADGIL
                     FALGQSFFTLSLGTTGMITYASYASKDMTIKSSAISIVVMNIFVSVLAGLAIFPALHS
                     FGYEPQEGPGLLFKVLPMVFSQMHLGTLFYLGFLVLFLFAALTSSISLLELNVSNFTK
                     NDNTKRKKVAVIGSILVFIISIPATLSFGILKDVRFGAGTIFDNMDFIVSNVLMPLGA
                     LGTTLVIGQLLDKKLLQQYFGKDRFRLFNGWYYLIKYAMPVVIILVFIVQLFS"
     gene            15043..15948
                     /locus_tag="PPY92_000574"
     CDS             15043..15948
                     /locus_tag="PPY92_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDJ2624597.1"
                     /translation="MITYDLIGNTPLVLLEHYSDDKVKIYAKLEQWNPGGSVKDRLGK
                     YLVEKAIQEGRVRAGQTIVEATAGNTGIGLAIAANRHHLKCKIFAPYGFSEEKINIMI
                     ALGADVSRTSQSEGMHGAQLAARSYAEKYGAVYMNQFESEHNPDTYFHTLGPELTSAL
                     QQIDYFVAGIGSGGTFTGTARYLKQHHVQCYAVEPEGSVLNGGPAHAHDTEGIGSEKW
                     PIFLERRLVDGIFTIKDQDAFRNVKSLAINEGLLVGSSSGAALQGALNLKAQLSEGTI
                     VVVFPDGSDRYMSKQIFNYEENDYE"
     gene            15941..17083
                     /locus_tag="PPY92_000575"
     CDS             15941..17083
                     /locus_tag="PPY92_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDJ2624598.1"
                     /translation="MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYE
                     YSRTANPTRSSVESVIAALENGKHGFAFSSGVAAISAVVMLLDKGDHIILNSDVYGGT
                     YRALTKVFTRFGIEVDFVDTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEI
                     AKEHGLISVVDNTFMTPYYQNPLDLGIDIVLYSATKYLGGHSDVVAGLVATSDDKLAE
                     RLAFISNSTGGILGPQDSYLLVRGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHP
                     SIESHLNHDVHMAQADGHTGVIAFEVKNTESAKQLIKATSYYTLAESLGAVESLISVP
                     ALMTHASIPADIRAKEGITDGLVRISVGIEDTEDLVDDLKQALDTL"
     gene            17378..18403
                     /locus_tag="PPY92_000576"
     CDS             17378..18403
                     /locus_tag="PPY92_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HDJ2624599.1"
                     /translation="MIEFRQVSKSFHKKKQTIDALKDVSFTVNRNDIFGVIGYSGAGK
                     STLVRLVNHLEAASNGQVIVDGHDITNYSEKGMREIKKDIGMIFQHFNLLNSATVFKN
                     VAMPLILSKKSKTEIKQRVTEMLEFVGLSDKKDQFPDELSGGQKQRVAIARALVTNPK
                     ILLCDEATSALDPATTASILTLLKNVNQTFGITIMMITHEMRVIKDICNRVAVMEKGQ
                     VVETGTVKEVFSHPKTTIAQNFVSTVIQTEPSTSLIRRLNDEQVGGFKDYKIFVEETQ
                     VTQPIINDLIQICGREVKILFSSMSEIQGNTVCYMWLRFNIDQQFDDTAINQYFKEKN
                     IQFEEVH"
     gene            18407..19066
                     /locus_tag="PPY92_000577"
     CDS             18407..19066
                     /locus_tag="PPY92_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDJ2624600.1"
                     /translation="MFGSDLDSAQLLQALYETLYMVSIALFLGAVIGIPLGVLLVITR
                     KQGIWPNIVIHQVLNPLINILRSLPFIILLIAIVPFTKLVVGTSIGTTAAIVPLTVYV
                     APYIARLVENSLLEVDEGIIEAAKAMGASPLQIIRYFLIPEALGSLVLAITTAIIGLI
                     GSTAMAGAVGGGGIGDLALVYGYQRFDTTVIIITVIVLVIIVQVIQTLGNVLARFIRR
                     H"
     gene            19103..19945
                     /gene="gmpC"
                     /locus_tag="PPY92_000578"
     CDS             19103..19945
                     /gene="gmpC"
                     /locus_tag="PPY92_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter
                     glycylmethionine-binding lipoprotein"
                     /protein_id="HDJ2624601.1"
                     /translation="MKRLIGLVIVALVLLAACGSNNDKKVTIGVASNDTKAWEKVKEL
                     AKKDDIDVEIKHFSDYNLPNKALNDGDIDMNAFQHFAFLDQYKKAHKGTKISALSTTV
                     LAPLGIYSDKIKDVKKVKDGAKVVIPNDVSNQARALKLLEAAGLIKLKKDFGLAGTVK
                     DITSNPKHLKITAVDAQQTARALSDVDIAVINNGVATKAGKDPKNDPIFLEKSNSDAV
                     KPYINIVAVNDKDLDNKTYAKIVELYHSKEAQKALQEDVKDGEKPVNLSKDEIKAIET
                     SLAK"
BASE COUNT         6843 a         2916 c         4007 g         6304 t
ORIGIN      
        1 attaaagcag agagatgtga aatgatggag tatataaaaa aaattgcttt gtacatgagt
       61 gtattacttt taatcatttt tattggggga tgtggaaata tgaaagaaga acagaaaaaa
      121 gaagcaaata caaataaaac agattcaaaa gaagaaaaaa tcaaaaagag ttttgcgaaa
      181 acgttagata tgtatccaat taagaatctc gaggaactat atgacaaaga aggctatcga
      241 gatggcgaat ttgaaaaagg tgacaaaggg atgtggacga tatatacaga tttcgccaaa
      301 agtaataaac cgggtgaact aagtaatgaa ggcatggtct tgtacttaga tagaaacaca
      361 cgcacagcta aggggtacta ctttgtcagg acgttttatc gcaaggataa attacctgat
      421 agaaaaaatt ataaagttga aatgaaaaat aataaaatta tcttattaga caaggtagaa
      481 gatccaaatc taaaaaagag aatagaaaac tttaaatttt tcggacaata tgcaaatctt
      541 aaagaattga aaaactacag caatggtgat gtctcaatta atgagaatgt tccaagttat
      601 gacgtgaaat ataaaatgag taataaagat gaaaatgtta agcaattaag aagtcgttat
      661 aatattccta ctgataaatc accggtatta aaaatgcata ttgatggtaa tttgaaagga
      721 agttccgttg gagataggaa gttagaaatt gatttttcga aacgtgaaaa tagccattta
      781 tcagtaatag attctttgga ttaccagcca gcgaaggtgg atgaagatga aagataaata
      841 caaaatagat ccagggatta ttaaaaataa tacagaggaa actacagcta tttctaaaat
      901 cagctacgaa gttgaaaatg cgaatttata tggagctgat agtgaagata taacaagaca
      961 aatagaatat ttgaaagcca aaaaaaaatt cccttctaat cttgaatatg ttgatagtta
     1021 cactgattct cttaatggag taacaacttc tgctttttta aataaagata caggcaaagt
     1081 aactcttggg atgactggga ctaatataca aggcgaagcc tttaaaaagt taaaagaagg
     1141 tgaattttca agacaaaatg ttaccaatgc tttggaaaca gttaaagatg gatatgcaga
     1201 tcttaaaata ttatattctc ctgcatctga tcaaaactat agatatgcga atacacaaga
     1261 atttataaat aaaataaaaa gtaagtatga cattgatttt attactggtc attcactagg
     1321 tggaagagat gcggtagttc ttggaatgag taatggtatt ccgaacattg tggtttataa
     1381 tccagctcct atttctataa atagtttgaa tcctaattcc ccagatggaa aacgattgtt
     1441 agagttatat aaaaattata aaggtaatat tactaggttt gttgcagaaa atgatgcatt
     1501 gacagaaaat ctgaagaaat ataagcatta tgtttttttc ggtaatgata aagtctttaa
     1561 aaatggtaaa ggtcatgaaa tggaaggctt tctgaccgaa gaagaacaaa aagctataaa
     1621 aaaagaactt aaaaaactac aaggttatgc agaagaaaat aataagtcat ttgtaaagaa
     1681 ttcaaataat gctatctcta aattagctag tatagaatta cttagagcaa atatgatgac
     1741 tacaaatgga ggaggattat cttcttcgca gcaaaaagtt ttagaaagtt taacagcttt
     1801 aacaattgcg cagtcattca gtcaactgat agacgatgaa attaatcaaa tcaaaaaaat
     1861 gtataatgaa aagaaaaagg aatttggaaa aaattgggaa gacgctcaaa aagctggaaa
     1921 agctgtaggt gaagatttga gtgtaaatgg agttctcaat gctttagatg aaggtcaagt
     1981 gaatgaaagt agtatggtaa gagaacctga acaaatgata tctgcaaaag aaagacaact
     2041 ttcaacgata gggtcttctg tatcaaatta tattatgaga gttagactca gtattaatga
     2101 aatcgttgat aaagatcaag tgcttgcatc acaaataggt ggattactat gatgtttaat
     2161 caaattaata ataaaaatga attagaagaa tcatatgaat ctgagaaaaa acgtatagag
     2221 aatgaactgc ataatttaaa tgaacttagg catagaactc gaaaagaaaa tgaacgtagt
     2281 tatgatgttt ttcaatattt gaagcacgaa atgaattata gtgaagatgc ccaaaggaaa
     2341 atgacgagaa atatagaagc gtatgagcaa gaaatcaatg agataattag aaagcaagaa
     2401 tggaaattag aagaatataa agaagactta aaaaagtctt atgaaaagca gttagataaa
     2461 ctaagtgact gataatttgg aggagattaa atgactctaa tagaaccaga tatgaactta
     2521 agaatgccag atataagtac tacagtagaa acacttaatc tcatatctaa aatggaagcg
     2581 caaaaagaaa acattcgttc agttattgca cctgaacata agcataaata taaagatatt
     2641 gaaaacggat taaaaggtga agaaaaagta ttgattgaac aaatggcgca gcattgcgaa
     2701 gcttttaaag ctaattttaa aggtgcagct caaggagact gggttaaaag tgcaatgtct
     2761 gagatagaca gtattaaaga tgatttgaaa aaaattaata gctaaactac atatcatact
     2821 actaaataaa gcgaccaatg ttcagtatat tcacaactga cactaggccg cttcttttta
     2881 atttatttta tttccatgta aagtaacgct ctcttaatcg aaataccaca ctcacaattg
     2941 taaaaacaca acttaaatta ctgtcccgca ttaaacatat ctacattcca aacagcaaca
     3001 acaaaatcct caagtaccaa aagctatgac cactttatca aaatcaacgc aagttaacct
     3061 ctcccgaact acccgctata gatcatcatc accttacaaa ccataaagcg ctgcagttaa
     3121 gtattgaata gcatagccaa ttgaaatgaa aatagcaatc gttataatgg cactaccaat
     3181 tgtatataaa gtatgtttcg tagataaacc ttccgtctta gaaccaaaaa cacttagtag
     3241 aataaatgca atgccaatga catataaaac taaaatgaca aataacatgt ttatacacct
     3301 cgattctctt tagtgagata tatctttgtc tccattataa agtaaagtgc tcaataattt
     3361 aattaagtat actatttttt tattatcgaa gattgcgaaa gaggaaacgc tcctatataa
     3421 tgtaaaacgg aattattcct atttacaaat aaattgaagt gagggtatat atatggctaa
     3481 aattccagtt acggtattaa gtggttattt aggctcgggg aaaacaacat tgttaaatca
     3541 tattttacaa aatcgtgaag gtcgacgtat cgcggtaatt gtaaatgata tgagtgaagt
     3601 taatatcgat aaagatcttg tcgcagatgg tgggggacta tcgcgtacag atgaaaaatt
     3661 agtcgaactc tctaatggtt gtatctgttg tacacttaga gatgatttat taaaagaagt
     3721 tgagcgttta gtgaaaaaag gtggcatcga tcaaattgtt attgagtcaa cagggatttc
     3781 agagccagta cctgttgcac aaactttctc atatattgat gatgaacttg gcattgatct
     3841 tacagcgatt tgccgtttag atacaatggt tacagttgtt gatgctaacc gcttcgtaca
     3901 tgacatcaac tcagaagatt tattgatgga tcgtgatcaa agcgttgatg aaacagatga
     3961 acgttcgatt gctgatttat taattgacca agttgaattt tgtgatgtat tgattattaa
     4021 taaaattgat ttaattagtg aagaagaact tgcaaagtta gaaaaagtgt taagcgcatt
     4081 gcaacctatt gctaaaatta ttaagacaac aaattctgaa gtagatttaa aagaagtctt
     4141 aaatacgcag cgttttgatt ttgaaaaagc gagcgagtca gcaggatgga tcaaagaact
     4201 tgagtcgggt ggtcatgcat cgcatacgcc tgaaacagaa gaatatggca tatcatcatt
     4261 tgtatataaa cgtcgtctac ctttccatgc taaaaggttc aatgattggt tagaaagcat
     4321 gtcaaataat gtcgttcgat caaaaggtat cgtatggtta gcacaataca atcatgtagc
     4381 atgtttatta tctcaagcag ggtcatcttg caatattcac ccagttacat attgggtggc
     4441 tagtatgtct gaagcgcaac aaacacaaat attagcagaa cgtcaagatg tcgcagctga
     4501 atgggatcca gaatatggcg atcgtcatac acaatttgtc attattggta cagaattaga
     4561 tgaagaaaaa ttaacaaaag aactcgatgc atgcttagtc aatgcgcaag aaattgatgc
     4621 agattggcaa caatttgaag atccatatca atggcaaatt agaccagcac gataagttga
     4681 atgaagtata atcatttttg aattgtggct taattgtttg caatttatga caattaataa
     4741 aaagttaaga tgttcttagg aactttttgt tttcaatcta tctaaagtgt gatagttttg
     4801 ataaagcaga aatttgcatt ttatccatag ggctaggaca tgtatgtgtc ttagtccttt
     4861 ttatatttgc gttgatgaaa aatgataaaa ccatcgtacc aactgttaat tgaaagtaca
     4921 attattgttt aacggtcata aaaatgagtg aagtaattgc agctatcaat gtactattac
     4981 tgcttacagt cacacgagtg tttcagacta aacgtgcaac gaaattctgc acgcggccaa
     5041 tatatcttgc gccagatcaa gaagatcatt aagatttcgc acgccaaatc atgaatgtct
     5101 tagacttcga gtatcaaaca taaaacacgc cacaaaaata taacgcgtca gactacaaag
     5161 atcacgaata ttccttacgt accaccaaga acatcctata caaaaaatac caagaccatt
     5221 tatttgcaac aaacaaacaa gactcacctc acatcaataa atcaacacaa agcaaagcca
     5281 ccatccctat tggtatagtg gcctgagaat tttaagtatt caattcgctt aaattatttt
     5341 gcgaaaatgt cgataattgc tttgatgatt ttaatgatag tacctacaat agccatcgtt
     5401 ttgtcctcct gtatgttgat ttaacatagt gatgagttgt tgattgttaa tgtttgagat
     5461 tagttgttac ctaaaaattt accaagtaaa tctttaaaga atttgaataa ttttgctacg
     5521 aattccatgt gaatggcccc cttcaaataa gatgttcata tctatgagat ttttataact
     5581 tacttaccag tgaatttctc aattaatcct ttaatgaatt taatgattcc tgcaatgata
     5641 cccatgtgaa agacctcctt tgtttgttat gaaatcttat ttaccagtga attgttcgat
     5701 taagcttttg ataactttaa tgatgccagc gatgataccc attaagatta cctcctttgc
     5761 ttatgagtta acttcattgt acatagttat cttgtgcgta attgattttt ttcctaactt
     5821 gaattgttat gatcataact gcgaattttt tattttaaaa ggacgggaaa tactgcctaa
     5881 ctgtacaggt gcgtttacaa aatctttaca atcattggtt taatagaggt taagctcatg
     5941 aatttgacat gaagcagaaa tttaattata ttttatgtta taagtttaaa acgaataaca
     6001 cgttaggtct cgtctaggca aagcatattt atgaagatga attgaacaga cagaagtaac
     6061 attttatata catatgtgca tgggagcacg cttaaagaga acactgcaac aaggtatatc
     6121 aacgtaccgt gatgcagtgt ctagttaaac tgaaacctgc aatagtaaat tttctgccat
     6181 tatgtacaga atctactatt gtaggttttt atttttatac ataagcaatt atgcgagaga
     6241 ttagaaataa ggaggttatg cagtgttaag ttttcaattg ctattttcac tgtttgttat
     6301 tgcgcttatc attgcattga taagtggctt gttgttttta gcaccagtta tgccaatgag
     6361 atatattaaa ttacatttat acatactagt catgccagta ttatttgcag tcattggctt
     6421 tttcggtatt catggtcaac atgtcttagg tccatttaaa atagatcgtt tatcttggtt
     6481 attagctggc tttgtaatgg cgcttggttt tattattcaa aagttttcaa tgcgatattt
     6541 actaggtgat catcattata gacattactt tccattgttc actgcgatta cgtcgtttgc
     6601 atctttagca tggatgtctg aagacttaag actgatggca ctctgctggg gtatgacatt
     6661 attatgttta acactgctga tgaacgttaa tcgtttttgg aaagtgccac gtgagtctgc
     6721 gaaattatca agcatgacat ttttatgtgg ttggcttgca ttcgttggag caattgtaac
     6781 tatttatatt gcgactggtg agtggcgggt accacaacat atagtacatc cgacatggtc
     6841 attgttgacg aatgtactac ttgtattagc tgtcatgata ccggcagcac aatttccttt
     6901 tcatcgatgg ttgattgaat ctgtaacggc accaacgcca gtatcggcaa ttatgcatgc
     6961 aggaattgtg aatgcaggtg gtgttattct aactcgtttt gcgccgatat ttgataatgg
     7021 atttgcgtta tcattattac ttatcctttc tagtatttct gtattgttag gatcgggtat
     7081 tagcttggtt caagttgact acaaaagaca gttagtgggc tctacgatga gtcaaatggg
     7141 ctttatgtta gttcaatgtg cattgggtgt atattcagca gcgattattc atttaatatt
     7201 gcacggtatt tttaaagcaa cattattttt acaatcaggt tctatcgtga agcgattcaa
     7261 tattccaaaa caagcatctg ctaaagacgc ttatggctgg attgtcatgg gacgtgtatt
     7321 agctattatc gtggcatttc tattttggat gagtagtgac agaagtgcat atgaagtgtt
     7381 aagtgcactc attctagcat ggtcattact tgtatcttgg aatcagatgg tagcctttag
     7441 taaagggcgc atggcacgtc tggttggcat gattttgatt gcaattgtga catttatcta
     7501 cgtcatcaca cacaattatt tttacgatgt attacaaaat ataacaacac atgcgacaac
     7561 accgcctaca gtgagtgtca tcattagtgt tgtcatttta atctttggca gtttattaag
     7621 tatttgggtg gcgcgtcatc gatactctaa gggttttgcg gtattgtacg tgtggttagt
     7681 taatctaggt gaagcacgct cgaaagcgat agaaagtcat ccgaattatt tgaagaagta
     7741 tttataggag gtgaaaggta tgacaacaca gttaaatatc aattcagtca ttgaaaatgc
     7801 gaaacgtgtt attacaccat tatcaccgat ttcgattttt gcagcacgta acccctggga
     7861 aggattagaa gcggatacgt ttgaagatgt cgcaaaatgg ttacgtgacg ttcgcgatgt
     7921 ggatattttc ccaaataaag cattaataga aagcgctgtg gcacgtggtg aattagatga
     7981 aagtgtcttt aatcaacttg ttactgatat gttacttgaa catcactaca atatcccgca
     8041 acactacatc aatctttata ttgataacat taaaacatta aaagacgtac ctgcatcata
     8101 tatgaatcat tcaaatgttg atgttgttgc tgatctacta ttagaaaaat caaaacgtga
     8161 tatggctgaa tcatatcatc actatgatgt acgtccgatg agtgatgcaa taatagatga
     8221 acaaggtgag ccacttagcg aacaagtgaa tcgtcaaatg attaaatgga cgaaacttta
     8281 tatcgatcaa tttctatcga gttggacaat gccgaagcgt gagcaaagtt tttaccatgc
     8341 atggttgcat ttagcgcaac atgaccatag ttttactaaa gcacagcgcc aagtgattaa
     8401 aggcttaccc aatgatcctg aaatgacgat agagtcagta ttaacttatt tttcaataga
     8461 tcaggaagac taccaagctt atgttgaagg acatcttttg gcgttaccgg gttgggcagg
     8521 tatgttgtat taccgttcac aacagcatca ctttgaacaa catttgttaa cggattattt
     8581 ggcaattcgg ttagttgtcg aacaattgct agttggtgat gagtttaagt cagtcactaa
     8641 agattgtgaa agtagatcgg aaaattggtt taagcaaact gttgcatcat tgtgttacta
     8701 cagtgatatg cctagcgatg tattactaca acatgacgtc aatgaaattc aaacgtttat
     8761 tcattttgca gcaactatga ataaaaatgt atttaaaaat ttatggctaa ttgcctggga
     8821 aatgacatac gaatctcagt taaaacaaaa aattaaagca ggtcatgaaa gtgtggcggg
     8881 cgcattagat gtaaaccaag taaatgtctc agaaaatgat aacgctaatc agccacattc
     8941 agtattgtta aatgacacac aagcagttga tgaaaataat agcgagctaa atcaggtggg
     9001 cacatcaacg aaagcgcaaa ttgcattttg tatagatgtt cgttcagaac catttcgtag
     9061 acatatcgaa gcagcagggc cctttgaaac gattggtatt gcaggtttct ttggattacc
     9121 tattcaaaaa gatgccgtag acgaacaatt caaacatgat tcattacctg tcatggtacc
     9181 gccggcatat cgcattaaag aatttgcaga ccgctacgat atgaatgttt atcgacaaca
     9241 gcaacagaca atgtcatcga tgttttacac atttaaattg atgaaaaata atgttatgcc
     9301 tagtctgtta ttgcctgaat taagtgggcc atttttaagc ttgagtacca ttgtcaattc
     9361 gattatgcct agaaaaagtc gcgcgtcttt acaaaaaata aaacaaaaat ggttgaaaaa
     9421 gcctgaaaca aagttaacga ttgatcgtga gtttgaccga acatcagact tacctgttgg
     9481 atttactgag caagagcaaa ttgatttcgc gttacaagcg ttgaaattga tggatttaac
     9541 cgaagcattt gcgccgttcg ttgtgttagc aggtcatgct agtcattctc acaataatcc
     9601 acatcatgca tcacttgaat gtggggcttg tggtggcgca tcaagcggtt ttaatgctaa
     9661 gttattagcg atgatatgta atcgtccaaa tgtcagacaa ggattaaaac aatcaggtgt
     9721 gtatattcca gagacaactg tttttgcggc agcagaacat catacgtcta ctgatacgtt
     9781 ggcatgggta tatgtgccag acacattatc agctttagct ctagatgcat atgaatcatt
     9841 gaatgacgcg atgccgatga tttctgaaca agcgaatcgc gaacgtttgg acaaactgcc
     9901 aacgattggt cgtgtgaatc atccagtgga agaagcacag cggtttgcaa gtgattggag
     9961 tgaggtacgt ccagaatggg gcttggctaa aaatgcatca tttataattg gacgacgcca
    10021 attaacaaaa ggcattgatt tagaagggcg gacattttta cacaattatg attggcgtaa
    10081 agataaagat ggcaaattgt taaataccat catttctggt ccggcgcttg tggcacaatg
    10141 gattaattta caatattatg cgtcgacagt tgcgccgcat ttttacggaa gtgggaataa
    10201 agcgacacaa accgtcacgt caggtgttgg tgtcatgcaa ggtaatgcga gtgatctgat
    10261 gtatggctta tcatggcaat ctgttatggc tgctgatcgg acgatgtatc attcgccaat
    10321 tcgtttactt gtcgttattc aggcacccga ctatgttgta gctagactat tcgcgaataa
    10381 tgagcatttc gctaggaagg tgtctaatca ttggctgcgt ttaatgagcg ttaatgagga
    10441 agggcgtttt aaaagttgga tttaacgatg atatgttgcg atgctaagta attaatatat
    10501 catcatatct acaatgttct agcttcttaa aatatactaa aaatgataaa cttaatgtaa
    10561 tagttgatgt cgtgataacg atacaaagat gaagtaatca attttaatat tattggagtt
    10621 agtgatatga aaagaacgaa aggtgaaatc gaagctgaaa tcagtaaagc cattacgcaa
    10681 tgggaaaaag atttccttgg cagaggttcc ttgtcagtta aatcagacat tttaagagat
    10741 atggtgatta ttagtttaca aggtatctta acgccagcag aatatcgtgt gtgtagtacg
    10801 aatgaaggat tactaaatat taaacgaaca cgttctgaat tagttgaatc cggtgagcaa
    10861 gatttgaatg atatcattct taaaattaca ggtatcaaag tgatgagctt ccacagtgat
    10921 ttaagtacag ttacaggtga acgtattatc gtattcaaac ttgaggataa tttggaaaag
    10981 catatttaag aaggttaggt atgcttctgc tggcttatta aagctataga agtatgcttg
    11041 gttaaacagc gcaacgccat atatttgggt gtgctgtttt tctattacat gatgtgttta
    11101 ttacgtggat gaatgcgtgg gtctgaatat agcccgtgaa aggcgtatgt cataaaattt
    11161 gcgaatggtg acaagtcaac gcaagcatat ttgttgcgag acatgcccac tatcctttta
    11221 cataaagagt atatgtgtga cgtaggcata taatcgataa agtataccta aaaaattagg
    11281 tataatgacc attgttgcaa taagtttttc ggccttgaaa ccgataacgc atagtataat
    11341 aggaataaaa tataatacaa atatccagta aatcaaattt gaaaacgatg gatgaatagg
    11401 ggtaaagaat ctaactaatg taatgattaa cgctatatag acaaaaataa ctctattgat
    11461 acgccttacc ccctctgtat aataaatata gatcgtacta aattgaaaat agcaaaatat
    11521 tgttatttca attatacaat gttttatttg caatatacat aacaatatga actttataat
    11581 tttgatattg tcggtaaagt gaaatttgtg gcacattgat attatggata acatttgtaa
    11641 aatgaaaatg atgatacgag gagattgtaa gatgatagat aaaaaattaa catcaccgaa
    11701 aatgacagtg cctttatttt taatcgcgct gattgtattt ataggtatgt tttacggtgt
    11761 agtgacaaat caagaatggc ttaaaaatat agatatggga tcattaacat ggtttacaga
    11821 ttatttcggt gagccacaac gtcagtatgt taacaatttg tttaattatt atatgacgtt
    11881 tagtgcggaa attggagatg tcaaaggtgt cgtgttgatt tcaattatcg tcacaatcat
    11941 actgtttatt aaacagaggc atttagcggt ttggtttgtg acatatttgg tttcaggtgt
    12001 catcatgaac aaattaatta aagatactgt tttacgtcca agaccatata atcatttagc
    12061 cgttgataca ggcttttcat ttccaagtgg acattccaac gccagcacat tattatattt
    12121 cgccttaatg atcattatta tttcagttgc tgctaagaca ataacaaaag tgttgagtgc
    12181 gctagttatg ggaatattat ggcttagcat attattttgt cgcctttatt ttcatgcaca
    12241 ttacttttca gatgtcattg gcggcacgtc actagcaatc atttgggtag cgttattctt
    12301 aatggtatac ccatacttta ttaatcatcg acgacaacgc gtttagcaaa catctttaga
    12361 catagtggag gtatatagaa aaatgagaat taaaacaccg agtccatctt atttaaaagg
    12421 cacaaatgga catgcgatat tattattaca ttcatttaca ggtacaaatc gggatgtgaa
    12481 gcatcttgca gctgagttaa atgaccaagg atttagttgt tatgcaccga attatccagg
    12541 tcatggttta ctgttgaaag atttcatgac atataatgta gatgattggt gggaagaagt
    12601 tgagaaagct taccaatttt tagtcaatga aggttatgaa tctatcagtg caacgggtgt
    12661 gtctttaggt ggattaatga cattaaaatt ggcgcagcac tacccgttga aacgtatcgc
    12721 tgtcatgtca gcaccaaagg aaaaaagtga tgatggctta atagaacatt tagtttatta
    12781 tagtcaacgc atgtcgaata ttttaaattt agatcagcaa gcatcgagtg cgcaattagc
    12841 agcaattgat gattatgaag gtgaaattac gaagtttcag cattttattg atgatatcat
    12901 gacaaattta aatgttatta aaattccagc taatatatta tttggtggta aagatgagcc
    12961 atcctatgaa acaagtgcac attttattta tgaacattta ggatcagtag acaaagaatt
    13021 aaatggtctg aaggattcgc atcatttaat gacacatgga gaaggcagag atattttaga
    13081 agaaaatgtt attcgctttt tcaatgcttt aacataattg aagattttaa atattaacac
    13141 ttgagtgacg atgttattat atttgtcatt tgagtgtttt ttgtggcgtg atagtatagg
    13201 gaacttagac aaagttaatt tgtagtgtga tgtcgtggaa atattatggg cattgtacct
    13261 gaagttgtta tgtggcgaga tattggcgca cgtatacagc gttgaaccag aaattttaat
    13321 gtagcgtaat gacaacacat tacaagagtt gactatatta tgataaatac aggcgctacc
    13381 gtattaatga aacaattttg atgcaggtgt attgcatgtt atttacatac atgttagagt
    13441 gttacttaaa cacgtagacg ctttaacgct ttaaaggaga tgtgaatgat gaaaagacaa
    13501 caatcacaat ggaagtcatc aactggattt attttagcta gtgcgggttc tgcaatcggt
    13561 cttggtgcca tgtggaaatt cccatatatg gcagggattt atggcggcgg tgcctttcta
    13621 gctatgttct taatattcac catttttgtt gggttgccat tactcattat ggaattcact
    13681 gttgggaaaa tgggacggac atatacaaca caaatatata gtaaattaac tggtaaaaaa
    13741 tggctcaata tcattggctg gaacggtaat ttggccgtgt ttattttatt tggcttctat
    13801 agtgttatcg gtggttggat tgtcatttac atcggacaag tattatggca attagttata
    13861 tttcaacgca tcaatcatct ccaagaaatg aattttgaag cggtaatatc aaatccttgg
    13921 ttaactgttc tagggcaagg tatattcata ttcgctacga tgattattgt catgttaggt
    13981 gttgaaaaag gattagaaaa ggcatcgaaa gttatgatgc cattgctgtt tgtcttttta
    14041 atcatcattg tgattaagtc tttaacatta gatggtgcct tagaaggtgt gaaatttatt
    14101 ttacaaccaa gagtatcaga gattactgct gatggcatct tgtttgcgct aggtcaatca
    14161 ttctttacgt tatcattagg aactacaggt atgattactt atgcgagtta tgcctctaaa
    14221 gacatgacga ttaagtcatc agctatttct atcgttgtta tgaatatctt tgtatctgta
    14281 ttggcaggcc tagctatatt tccggcgtta catagttttg gctatgaacc acaagaaggg
    14341 cctggattat tatttaaagt actgccaatg gtctttagtc aaatgcatct aggcacatta
    14401 ttctatttgg gattcttagt gctgttctta tttgcggctt taacgtcatc tatttcttta
    14461 ttagaattaa atgtttctaa cttcacgaag aatgacaata caaaacgtaa aaaagtcgca
    14521 gtgatcggta gtattttagt atttatcatt agtattccag caaccttatc ttttggtatc
    14581 ttaaaagatg taagattcgg tgcgggaacg atttttgata atatggattt catcgtttcg
    14641 aatgtattga tgccattagg cgcattaggt actacgcttg tcataggaca attattagat
    14701 aaaaaattat tacaacaata ttttggtaaa gatcgattta gattattcaa tggttggtat
    14761 tacttaatta agtatgcgat gcctgtcgtt attattttag tctttatcgt gcaattattt
    14821 agttaatata tagaagcatc tgacacacaa gtatttgtgt tgctcaggtg tttttttgtt
    14881 tggaattgaa aagttagtac tggttctgaa ttaatcgaaa cttagataat tgcaaaataa
    14941 caaaatgtcg tttataattc ttatctgaaa agtaagaatt aaaattgaga catgaagaaa
    15001 ggtttcattc atttgatcgg catgtcagat gagggtgcat ctatgattac ttatgattta
    15061 attggcaata caccattagt actgttagaa cattatagtg atgataaagt taaaatttat
    15121 gccaagcttg aacaatggaa tcctggaggc agtgttaaag acagactcgg gaaatattta
    15181 gtagagaagg caattcaaga agggcgtgtg cgtgcaggtc aaactattgt tgaagcgact
    15241 gctggtaata caggcatagg gttagctatt gcagcgaata gacatcattt gaaatgtaag
    15301 atctttgcgc cgtatggttt ttcagaagaa aagattaata ttatgatagc gcttggtgca
    15361 gatgtttcaa ggacgagtca gtctgaaggt atgcatgggg cacaattagc tgcacgttcc
    15421 tatgctgaaa aatatggtgc cgtttatatg aatcaatttg aatccgaaca taatccggat
    15481 acatattttc atacattggg acccgaattg acttcagcat tacagcaaat tgattatttt
    15541 gtggctggta ttggctctgg cggtacattt acaggtaccg cacgttattt aaagcaacat
    15601 cacgtgcaat gttatgccgt tgagccagaa gggtccgtgt taaatggagg gccagctcat
    15661 gcacatgaca ctgaaggtat cggttctgag aaatggccga tatttttaga gagacgtctt
    15721 gtagatggga tatttacgat taaagatcaa gatgcctttc gaaatgtcaa aagtttggct
    15781 ataaatgaag ggttgttagt aggcagttct tcaggtgcag cattacaagg tgcattgaat
    15841 ttaaaagcgc aattatctga aggtacgatt gttgtcgtat ttccagatgg tagcgatcga
    15901 tatatgtcta agcaaatatt taattatgag gagaatgatt atgaataaga aaactaaatt
    15961 aattcacggt ggacacacaa cggacgacta tacaggtgca gttactacac caatttatca
    16021 aacaagtaca tatttacaag atgatattgg tgatttacgt caaggatatg aatattctcg
    16081 tactgcgaat ccaacaagaa gttctgtaga aagcgttatt gcggcattag aaaatggcaa
    16141 acatggcttt gcgtttagtt caggtgttgc agctatcagt gcagttgtta tgctgttgga
    16201 caaaggtgat catattattt taaattcaga tgtatacggc ggtacttatc gcgcattgac
    16261 aaaagtattt acacgatttg gcattgaagt ggattttgta gatacaacgc atacagattc
    16321 aattgtacaa gcgatacgcc caacaacaaa gatgttgttt attgaaacac cttctaatcc
    16381 attattacgt gttactgaca ttaaaaagtc tgctgaaatt gcgaaagaac acggtttgat
    16441 ttcagtcgtt gataacacat ttatgacacc ttattatcag aatccattag atttaggcat
    16501 cgatattgtc ttgtattctg caacgaaata tttaggtgga catagtgatg tcgttgctgg
    16561 tttagttgca acatcggatg acaagcttgc agaacgttta gcatttattt caaattcaac
    16621 aggtggcatt ttaggacctc aagatagcta tttacttgtg aggggtatta aaacattagg
    16681 tttacgtatg gaacaaatta atcgcagtgt tattgaaatt attaaaatgt tacaagcaca
    16741 tccagctgtg caacaagtgt tccatccaag tattgaaagt catttaaatc atgatgtcca
    16801 tatggctcaa gcggatggcc atacaggtgt gattgcattt gaagtgaaaa atacagaaag
    16861 tgccaaacaa ttgattaaag caacatcgta ttacacatta gctgaaagtt taggtgcagt
    16921 ggaaagttta atttcagtac ctgcattgat gacacatgca tccattccag cagatattcg
    16981 agctaaagaa ggtattacag acggacttgt aagaatttct gtaggtattg aagatactga
    17041 agatttagtc gatgatttaa aacaagcact agatacgcta taaataatag cagcactagc
    17101 atatatttta agatcaactt aaattgattt tgaaaatgct agtgcttttt tgtgcgatgt
    17161 taatcattcg tgattgagtt atggaggttt ggtggacatt tgtatggatt taatttgata
    17221 aatgtcatag tagtctcaca attcatcatt gtcacatgaa ttaattattt ttttaatttt
    17281 ttagaaaatt cggcaaattg tattgacgat atatgcaatt ttacatataa tgctcttatt
    17341 cctagtggat taataagaat ttgtaggagg ggcgatgatg attgagtttc gacaagttag
    17401 taaatcattt cataagaaaa agcaaacaat agatgctttg aaggacgtat catttacggt
    17461 caatcgcaat gatatttttg gtgtgattgg atatagtggt gcaggaaaaa gtacgttggt
    17521 aagactcgtg aatcatcttg aagctgcctc gaatggacaa gtgattgtag atggacatga
    17581 tattacgaat tatagcgaaa aaggcatgcg agaaattaag aaagatatcg gtatgatatt
    17641 tcagcatttc aatttattaa attcagctac ggtatttaaa aatgtagcaa tgccactcat
    17701 tttaagtaag aaaagcaaaa cagaaattaa gcaacgagta acggaaatgc ttgaatttgt
    17761 aggattgagt gataaaaaag accaatttcc tgatgaatta tctggtgggc agaagcaaag
    17821 ggtggctatt gcaagagcgc ttgttactaa tccgaaaata ctcctatgcg atgaagcaac
    17881 aagcgcattg gatccagcaa cgactgcttc gatattgacg ttattaaaga atgtcaatca
    17941 aacctttggc attacaatta tgatgattac acatgaaatg cgcgttatta aagacatttg
    18001 taatcgtgtt gctgtaatgg aaaaggggca agtggttgaa acaggaactg ttaaagaggt
    18061 gtttagtcat cctaaaacga cgattgctca aaattttgtg tctacagtta tacagactga
    18121 gccaagtaca tcattgattc gtcgattgaa tgacgaacaa gttggcggtt ttaaagatta
    18181 taaaatcttc gtcgaggaaa ctcaggtgac acaaccgatt ataaatgact tgattcaaat
    18241 ttgtggcaga gaggttaaaa ttttattttc atctatgtca gaaatacaag gtaacaccgt
    18301 atgttatatg tggcttcgat ttaatataga tcaacaattt gatgacacgg caataaatca
    18361 atatttcaaa gagaaaaata ttcaatttga ggaggtgcat taacatatgt ttggttctga
    18421 tttagacagt gcacagttat tacaagcatt gtacgaaacg ttatatatgg tatctattgc
    18481 tttattttta ggagcagtga ttggtattcc attaggtgtc ttattggtaa ttactcgaaa
    18541 acaaggcata tggcccaata tagtgataca tcaagtttta aatcctttaa tcaatatttt
    18601 aaggtcacta ccatttatta ttttgttaat tgcgattgtg ccattcacaa aattagtagt
    18661 aggtacttca attggtacga ctgctgccat cgtgccttta actgtatatg tggcacctta
    18721 cattgcaaga cttgttgaaa actcattatt ggaagtagac gaggggatta ttgaagcggc
    18781 gaaagcgatg ggcgcttcac cactacaaat cattagatat tttttaattc ctgaagcgtt
    18841 aggttcatta gtattagcaa ttaccactgc gattattgga cttattggaa gtacggcgat
    18901 ggcaggagct gttggcggtg gtggtatagg agacttagct ttagtgtatg gttatcaaag
    18961 atttgatacg acggtcatta ttattaccgt tattgtatta gtcattattg tccaagtgat
    19021 tcaaacgcta gggaatgtcc tagctagatt catacgtaga cattaatgat atatagtgaa
    19081 gattttgaaa ggaattgata gaatgaaaag attgattggg ttagttatcg tagcacttgt
    19141 attattagca gcgtgtggta gtaacaatga taaaaaagta acaattggtg tcgcatcaaa
    19201 tgacactaag gcttgggaga aggttaaaga attagctaaa aaagatgata ttgatgtgga
    19261 gattaagcac ttctccgatt acaatttacc gaataaagca ttaaacgatg gtgatattga
    19321 tatgaatgca ttccaacatt ttgcattttt agatcagtat aagaaggcac ataaaggaac
    19381 aaagatttca gcattgagta caacagtttt agcaccgttg ggcatttact cagataaaat
    19441 taaagatgtc aaaaaggtta aagatggtgc taaagttgtc attccaaatg atgtatcaaa
    19501 ccaagcacgt gcacttaaac tattagaagc agctggttta ataaaactga aaaaagattt
    19561 cggattagca ggtacagtga aagatataac gtcaaatcca aaacatttaa aaattactgc
    19621 agtagatgca caacaaactg cgcgtgcttt atctgatgtc gatattgcag ttattaataa
    19681 tggtgtagca actaaagcgg gtaaagatcc taaaaatgat ccgatatttt tagaaaaatc
    19741 aaattcagat gctgtaaagc catatattaa tattgttgca gttaatgaca aagacttgga
    19801 taacaaaaca tatgctaaaa tcgtagaatt gtatcattca aaagaagctc aaaaagcgtt
    19861 gcaggaagat gtcaaagatg gagagaaacc tgttaattta tctaaagatg agattaaggc
    19921 aatagaaacg tcattagcaa aataaattat attgcgtcct acaagcaaag ttcatgctta
    19981 tgtttgtagg gcgttattgt ttgacaataa aattatttcc aatagagaaa gggaatgtaa
    20041 tcattttata gtgaaatatt atgaaattgt
//
LOCUS       DANBWH010000023        19903 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.023, whole genome shotgun sequence.
ACCESSION   DANBWH010000023 DANBWH010000000
VERSION     DANBWH010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19903
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.023"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(57..224)
                     /locus_tag="PPY92_000579"
     CDS             complement(57..224)
                     /locus_tag="PPY92_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624602.1"
                     /translation="MKRIVILENVIVDNPIWLNHVDYLVKSVLLKSIKLRFQEVRKFE
                     ILVKIMNRIHI"
     gene            complement(352..855)
                     /locus_tag="PPY92_000580"
     CDS             complement(352..855)
                     /locus_tag="PPY92_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDJ2624603.1"
                     /translation="MSKWIIVSDNHTESGVLYQIYEMHPDADVYLHLGDSEFAYDDTE
                     LSLFNRVKGNCDFYPEFENEAVAKYNDVKAFYTHGHLYQVNRTRDLLAEKGLELGCLF
                     AFYGHTHVAKYEYINGVHVINPGSISQSRSSMEETYAEVIIDDQTLHGTINFKNRHHE
                     TISHTTF"
     gene            complement(848..1435)
                     /locus_tag="PPY92_000581"
     CDS             complement(848..1435)
                     /locus_tag="PPY92_000581"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HDJ2624604.1"
                     /translation="MKEIVIASNNQGKINDFKVIFPDYHVIGISELIPDFDVEETGST
                     FEENAILKSEAAAKALNKTVIADDSGLEVFALNGEPGIYSARYAGENKSDEANIEKLL
                     NKLGNTTDRRAQFVCVISMSGPDMETKVFKGTVSGEIADGKYGENGFGYDPIFYVPKL
                     DKTMAQLSKEQKGQISHRRNAINLLQAFLEGEKNV"
     gene            complement(1447..2247)
                     /gene="racE"
                     /locus_tag="PPY92_000582"
     CDS             complement(1447..2247)
                     /gene="racE"
                     /locus_tag="PPY92_000582"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDJ2624605.1"
                     /translation="MNKPIGVIDSGVGGLTVAKEIMRQLPNETIYYLGDIGRCPYGPR
                     PGEQVKQYTVEIARKLMEFDIKMLVIACNTATAVALEYLQKTLSIPVIGVIEPGARTA
                     IMTTRNQNVLVLGTEGTIKSEAYRTHIKRINPHVEVHGVACPGFVPLVEQMRYSDPTI
                     TSIVIHQTLKRWRNSESDTVILGCTHYPLLYKPIYDYFGGKKTVISSGLETAREVSAL
                     LTFSNEHASYTEHPDHRFFATGDPTHITNIIKEWLNLSVNVERISVND"
     gene            complement(2483..3298)
                     /gene="sdhB"
                     /locus_tag="PPY92_000583"
     CDS             complement(2483..3298)
                     /gene="sdhB"
                     /locus_tag="PPY92_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HDJ2624606.1"
                     /translation="MTEQSVKNTPQHETQSKPKQKTVKLIIKRQDTSDSKPYEETFEI
                     PYRENLNVIACLMEIRRNPVNIKGEKTTPVVWDMNCLEEVCGACSMVINGRARQSCSA
                     IVDQLEQPIRLEPMNTFPVIRDLQVDRSRMFDNLKRMKAWIPIDGTYDLGPGPRMPEK
                     KRQTAYELSKCMTCGVCLEVCPNVTENNKFVGAQAISQVRLFNLHPTGSMTKDERLNA
                     LMGTGGLQQCGNSQNCVNACPKGIPLTTSIAAMNRETTFHMFKSFFGSDHEVE"
     gene            complement(3298..5064)
                     /gene="sdhA"
                     /locus_tag="PPY92_000584"
     CDS             complement(3298..5064)
                     /gene="sdhA"
                     /locus_tag="PPY92_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HDJ2624607.1"
                     /translation="MAEKHLIVVGGGLAGLMSTIKAAEKGAHVDLFSVVPVKRSHSVC
                     AQGGINGAVNTKGEGDSPWIHFDDTVYGGDFLANQPPVKAMTEAAPKIIHLLDRMGVM
                     FNRTNEGLLDFRRFGGTLHHRTAYAGATTGQQLLYALDEQVRAYEVDGLVTKYEGWEF
                     LGIVKGDDDSARGIVAQNMTTAEIETFGSDAVIMATGGPGIIFGKTTNSMINTGSAAS
                     IVYQQGAIYANGEFIQIHPTAIPGDDKLRLMSESARGEGGRIWTYKDGKPWYFLEEKY
                     PDYGNLVPRDIATREIFDVCINQKLGINGENMVYLDLSHKDPHELDVKLGGIIEIYEK
                     FTGDDPRKVPMKIFPAVHYSMGGLYVDYDQMTNIKGLFAAGECDFSQHGGNRLGANSL
                     LSAIYGGTVAGPNAIDYISNIDRSYTDMDESIFEKRKAEEQERFDKLLAMRGTENAYK
                     LHRELGEIMTANVTVVRENEKLLETDKKIVELMKRYEDIDMEDTQTWSNQAVFFTRQL
                     WNMLVLARVITIGAYNRNESRGAHYKPEFPERNDEEWLKTTMASFQGAFEKPQFTYDD
                     VDVSLIPPRKRDYTSKSKGGKK"
     gene            complement(5116..5730)
                     /locus_tag="PPY92_000585"
     CDS             complement(5116..5730)
                     /locus_tag="PPY92_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b558 subunit"
                     /protein_id="HDJ2624608.1"
                     /translation="MAQSKNEFYLRRIHSLLGIIPIGAFLVVHLLVNHQATQGAEAFN
                     KASNFMESLPFLIIVEFLFIYIPLLYHGLFGIHIAFTAKENVGHYSIFRNWMFFFQRV
                     SGILTFIFIGIHLWQTRLQKAFYGKEVNYDLMHETLQHPGWAIFYIICIIAVVFHFAN
                     GLWSFLVTWGGLQSPKSQRVFTWVSLIVFLVISYIGVTAIIAFM"
     gene            complement(6054..7835)
                     /gene="uvrC"
                     /locus_tag="PPY92_000586"
     CDS             complement(6054..7835)
                     /gene="uvrC"
                     /locus_tag="PPY92_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDJ2624609.1"
                     /translation="MEDYKQRIKNKLNVVPMEPGCYLMKDRNDQVIYVGKAKKLRNRL
                     RSYFTGAHDAKTTRLVGEIRRFEFIVTSSETESLLLELNLIKQYQPRYNILLKDDKSY
                     PFIKITKEKYPRLLVTRTVKQGTGKYFGPYPNAYSAQETKKLLDRIYPYRKCDKMPDK
                     LCLYYHIGQCLGPCVYDVDLSKYAQMTKEITDFLNGEDKTILKSLEERMLTASESLDF
                     ERAKEYRDLIQHIQNLTNKQKIMSSDKTIRDVFGYCVDKGWMCIQVFFIRQGNMIKRD
                     TTMIPLQQTEEEEFYTFIGQFYSLNQHILPKEVHVPRNLDKEMIQSVVDTKIVQPARG
                     PKKDMVDLAAHNAKVSLNNKFELISRDESRTIKAIEELGTQMGIQTPIRIEAFDNSNI
                     QGVDPVSAMVTFVDGKPDKKNYRKYKIKTVKGPDDYKSMREVVRRRYSRVLNEGLPLP
                     DLIIVDGGKGHMNGVIDVLQNELGLDIPVAGLQKNDKHQTSELLYGASAEIVPLKKNS
                     QAFYLLHRIQDEVHRFAITFHRQTRQKTGLKSILDDIDGIGNKRKTLLLRSFGSIKKM
                     KEATLEDFKNIGIPENVAKNLHEQLHK"
     gene            8001..8096
                     /locus_tag="PPY92_000587"
     CDS             8001..8096
                     /locus_tag="PPY92_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624610.1"
                     /translation="MLDYHLTFIDLLIPPFSSRKQYDDLSFNFLY"
     gene            complement(8159..8473)
                     /gene="trxA"
                     /locus_tag="PPY92_000588"
     CDS             complement(8159..8473)
                     /gene="trxA"
                     /locus_tag="PPY92_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDJ2624611.1"
                     /translation="MAIVKVTDADFDSKVESGVQLVDFWATWCGPCKMIAPVLEELAA
                     DYEGKADILKLDVDENPSTAAKYEVMSIPTLIVFKDGQPVDKVVGFQPKENLAEVLDK
                     HL"
     gene            complement(8646..10994)
                     /locus_tag="PPY92_000589"
     CDS             complement(8646..10994)
                     /locus_tag="PPY92_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDJ2624612.1"
                     /translation="MRQKTLDVLEFEKIKSLVANETISDLGLEKVNQMMPATNFETVV
                     FQMEETDEIAQIYNKHRLPSLSGLSKVSAFIHRADIGGVLNVSELNLIKRLIQVQNQF
                     KTFYNQLVEEDEGVKYPILDDKMNQLPVLTDLFHQINETCDTYDLYDNASYELQGIRS
                     KISSTNQRIRQNLDRIVKSQANQKKLSDAIVTVRNERNVIPVKAEYRQDFNGIVHDQS
                     ASGQTLYIEPSSVVEMNNQISRLRHDEAIEKERVLTQLTGYVAADKDALLVAEQVMGQ
                     LVFLIAKARYSRSIKGTKPIFKEERTVYLPKAYHPLLNRETVVANTIEFMEDIETVII
                     TGPNTGGKTVTLKTLGLIIVMAQSGLLIPTLDGSQLSVFKNVYCDIGDEQSIEQSLST
                     FSSHMTNIVEILKHADKHSLVLFDELGAGTDPSEGAALAMSILDHVRKIGSLVMATTH
                     YPELKAYSYNREGVMNASVEFDVDTLSPTYKLLMGVPGRSNAFDISKKLGLSLNIINK
                     AKTMIGTDEKEINEMIESLERNYKRVETQRLELDRLVKEAEQVHDDLSKQYQQFQNYE
                     KSLIEEAKEKANQKIKAATKEADDIIKDLRQLREQKGADVKEHELIDKKKRLDDHYEA
                     KSIKQNVQKQKYDKIVAGDEVKVLSYGQKGEVLEIVNDEEAIVQMGIIKMKLPIEDLE
                     KKQKEKVKPTKMVTRQNRQTIKTELDLRGYRYEDALIELDQYLDQAVLSNYEQVYIIH
                     GKGTGALQKGVQQHLKKHKSVSDFRGGMPSEGGFGVTVATLK"
     gene            complement(11004..12716)
                     /gene="polX"
                     /locus_tag="PPY92_000590"
     CDS             complement(11004..12716)
                     /gene="polX"
                     /locus_tag="PPY92_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HDJ2624613.1"
                     /translation="MTKKDVIKLLEQIATYMELKGENTFKISAYRKAAQSLELDERPL
                     DEISDVTELKGIGKGVAEVINDYRETGESQYLQQLQEEVPEGLIPLLKIQGLGSKKIA
                     KLYKELNIVDKASLQVACENGKVSELSGFAKKTEQNILEAVKQLGAKKDRYPIDQMRR
                     LNQEIIDYIDTLNYIDQYSSAGSFRRFKEMSKDLDFIISTDNPKAVQQQLLNIPNKVK
                     EVAVGNTKVSLELAYDDETIGVDFRLIEPRAFYHTLQHFTGSKEHNIRIRQLAKARDE
                     KVSEYGIEQADGTLIQYDSEAKIYEHFNVNFIPPAMREDGSEFDKDLSNIITLDDING
                     DIHMHTTYSDGAFSIRDMVEANIAKGYKFMVITDHSQSLRVANGLQVERLLRQNEEIK
                     ALDKEYSEIDIYSGTEMDILPDGSLDYDDEILAQLDYVIGAIHQSFNQSEEQIMERLA
                     NACRNPYVRHIAHPTGRIIGRRDGYKPNIEQLMALAEETNTVLEINANPHRLDLNAEI
                     VRKYPNVKLTINTDAHHTNHLDFMNYGVATAQKGFVTKDRVINALSREAFKDFIENNI
                     KLKK"
     gene            complement(12789..13310)
                     /locus_tag="PPY92_000591"
     CDS             complement(12789..13310)
                     /locus_tag="PPY92_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDJ2624614.1"
                     /translation="MVIDFIIIIFFVYFVIVGFRRGFWLSMIHLSATIVSLWIASQFY
                     KSIVERLIVFIPYPKTTAFNTTFAFHFNHLQNRFEAIVAFLMITLFCKFILYLIIVTF
                     DKIIAYQNIHIFSRAMGMIVGVFMTIIVLHFTLYLLALYPNEALQHQLKISIVSHSLI
                     FHIPYLSAFTINL"
     gene            complement(13311..13577)
                     /gene="zapA"
                     /locus_tag="PPY92_000592"
     CDS             complement(13311..13577)
                     /gene="zapA"
                     /locus_tag="PPY92_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDJ2624615.1"
                     /translation="MAQFKNKVNVSINDQLFTIVGEDNPEHIRYVAHLVDDKIKELGY
                     KAAGLDTSRKAILTAVNIMHEKVLLEEENRRLKQQIHKLQQREQ"
     gene            13947..14885
                     /locus_tag="PPY92_000593"
     CDS             13947..14885
                     /locus_tag="PPY92_000593"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HDJ2624616.1"
                     /translation="MANIVFKLSDKDITTLMSRITFDTENLPQGMKARAKYQNTTVNI
                     YQSGKVMFQGNHAEAVSKELLPQHSQLNTNKTKKKNMANSSLEQTLMYDQFNCIGSDE
                     AGSGDYFGPLTVCAAFVTKEHVPILKTLGVDDSKKLTDTKIVELAEQLVAFIPHSLLT
                     LHNDKYNIQQAKGWTQVKMKAVLHNEAIKNVLEKIDSSQLDYIVIDQFAKREVYSHYA
                     LSDIPLPKKTKFETKGESKSLAIAVASIISRYAFITYMDQISKYINMTIPKGAGAKVD
                     VIAAKIIKKYGLSRLDTISKKHFKNREKAQKILKPL"
     gene            complement(15317..17719)
                     /locus_tag="PPY92_000594"
     CDS             complement(15317..17719)
                     /locus_tag="PPY92_000594"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDJ2624617.1"
                     /translation="MLISNEWLKEYVTIDDSVSDLAERITRTGIEVDDLIDYTKDIKN
                     LVVGFVKSKEKHPDADKLNVCQVDIGEDEPVQIVCGAPNVDAGQYVIVAKVGGRLPGG
                     IKIKRAKLRGERSEGMICSLQEIGISSNYIPKSFESGIYVFSESQVPGTDALQALYLD
                     DQVMEFDLTPNRADALSMIGTAYEVAALYNTKMTKPETTSNELELSANDELTVTIENE
                     DKVPYYSARVVHDVTIEPSPIWMQARLIKAGIRPINNVVDISNYVLLEYGQPLHMFDQ
                     DAIGSQQIVVRQANEGEKMTTLDDTERELLTSDIVITNGQTPIALAGVMGGDFSEVKE
                     QTSNIVIEGAIFDPVSIRHTSRRLNLRSESSSRFEKGIATEFVDEAVDRACYLLQTYA
                     NGKVLKDRVSSGELGAFITPIDITADKINRTIGFDLSQNDIVTIFNQLGFDTEINDDV
                     ITVLVPSRRKDITIKEDLIEEVARIYGYDDIPSTLPVFDKVTSGQLTDRQYKTRMVKE
                     VLEGAGLDQAITYSLVSKEDATAFSMQQRQTIDLLMPMSEAHASLRQSLLPHLIEAAS
                     YNVARKNKDVKLFEIGNVFFANGEGELPDQVEYLSGILTGDYVVNQWQGKKETVDFYL
                     AKGVVDRVSEKLNLEFSYRRADIDGLHPGRTAEILLENKVVGFIGELHPTLAADNDLK
                     RTYVFELNFDALMAVSVGYINYQPIPRFPGMSRDIALEVDQNIPAADLLSTIHAHGGN
                     ILKDTLVFDVYQGEHLEKGKKSIAIRLNYLDTEETLTDERVSKVQAEIEAALIEQGAV
                     IR"
     gene            complement(17719..18777)
                     /gene="pheS"
                     /locus_tag="PPY92_000595"
     CDS             complement(17719..18777)
                     /gene="pheS"
                     /locus_tag="PPY92_000595"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDJ2624618.1"
                     /translation="MSEQQTMSELKQQALVDINEANDERALQEVKVKYLGKKGSVSGL
                     MKLMKDLPNEEKPAFGQKVNELRQTIQNELDERQQMLVKEKLNKQLAEETIDVSLPGR
                     HIEIGSKHPLTRTIEEIEDLFLGLGYEIVNGYEVEQDHYNFEMLNLPKSHPARDMQDS
                     FYITDEILLRTHTSPVQARTMESRHGQGPVKIICPGKVYRRDSDDATHSHQFTQIEGL
                     VVDKNVKMSDLKGTLELLAKKLFGADREIRLRPSYFPFTEPSVEVDVSCFKCKGKGCN
                     VCKHTGWIEILGAGMVHPNVLEMAGFDSSEYSGFAFGMGPDRIAMLKYGIEDIRHFYT
                     NDVRFLDQFKAVEDRGDM"
     gene            complement(19157..19897)
                     /locus_tag="PPY92_000596"
     CDS             complement(19157..19897)
                     /locus_tag="PPY92_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDJ2624619.1"
                     /translation="MEQITSAQNNRIKQANKLKKKRERDKTGLALIEGVHLIEEAYQS
                     GIVITQLFAIEPARLDQQIIAYAQEVFEINMKVAESLSGTVTPQGFFAIIEKPHYDIS
                     KAQQVLLIDRVQDPGNLGTLIRTADAAGMDAVIMEKGTTDPYQDKVLRASQGSVFHLP
                     VMTQDLDTFITQFNGPVYGTALENAVAYKEVTSSDSFALLLGNEGEGVNPELLAHTTQ
                     NLIIPIYGKAESLNVAIAGSILLYHLKG"
BASE COUNT         6160 a         3829 c         2789 g         7125 t
ORIGIN      
        1 tactccatat tttaaccatc aaattattga tataatttat gactttagaa atccttttaa
       61 atatgtatac gattcatgat tttaactaaa atttcaaatt tcctgacttc ttggaatctt
      121 aattttatac tttttaataa tacactttta accaaataat caacatgatt caaccatata
      181 gggttatcaa caatcacatt ctccaaaatt acaattcttt tcataacata aatgtgttat
      241 tcattccaat tatcttataa aattgtcaga aatactttta aacaaacatt catgacacga
      301 cctcctagtt aaatcagtct ataaactaaa aaaagccata gctacttcta tttaaaaagt
      361 agtatgactg attgtttcgt gatgtcgatt tttgaaattg atggtgccat gtaaagtttg
      421 atcatcaata ataacttcag catatgtttc ttccattgaa cttctagatt gagatatact
      481 tccaggatta ataacatgaa caccattaat atactcatat tttgccacat gtgtatgtcc
      541 ataaaatgca aacaaacaac ctaattcaag tcctttttca gctaataaat ctcttgttcg
      601 attgacttga tataaatgtc catgagtata aaatgctttc acgtcattat attttgcgac
      661 cgcttcattt tcaaattctg ggtaaaaatc acaattgcct tttactctat taaataagct
      721 aagttccgta tcatcatacg cgaattctga atctcctaaa tgtaaatata catctgcatc
      781 tgggtgcatt tcataaattt gatataaaac gcctgattca gtatggttat cactcacaat
      841 aatccattta gacatttttt tcaccttcaa gaaaagcttg aagtaaatta atcgcatttc
      901 gtctatgact aatttgccct ttttgttctt ttgaaagttg agccatggtt ttatctaatt
      961 tcggtacata aaatatcgga tcatatccga aaccattttc gccatatttt ccatctgcaa
     1021 tttcacctga aacagtacct ttaaatactt ttgtttccat atcagggcca ctcatactta
     1081 tgacacaaac aaattgcgca cgacgatcag ttgtattacc aagcttattt aataattttt
     1141 caatatttgc ttcatcgctt ttattttcac cagcataacg tgcagagtat atacctggct
     1201 caccatttaa tgcaaaaact tctagtccac tgtcatcagc tatgacagtt ttattcaatg
     1261 cttttgcagc agcttctgat tttaatatag cattttcttc aaatgttgat cctgtttctt
     1321 ccacatcaaa atctggtatt agttctgaaa tacctattac gtggtaatct ggaaatatta
     1381 ctttaaagtc atttattttc ccttgattat tcgatgcaat aacaatctct ttcattaaaa
     1441 atcctcctag tcattcactg atatacgttc cacattgaca gataaattta accactcttt
     1501 gataatgtta gtaatgtgag taggatcacc tgttgcaaaa aatcgatgat ctggatgttc
     1561 agtataactt gcatgttcat tactaaatgt tagcaatgca ctaacttcac gagccgtttc
     1621 taatccagac gaaatcactg tctttttacc accaaaataa tcatagatag gtttatagag
     1681 caatggatag tgggtacatc ctaaaatgac agtatcagac tcactattac gccaacgttt
     1741 cagtgtttga tgaatgacaa tgcttgtaat tgttggatca ctatatctca tttgttctac
     1801 aagtggcaca aaacctggac aggcaacgcc atgtacctct acatgtggat tgatacgttt
     1861 aatatgcgta cgatatgctt cagatttaat tgtgccttca gttcctagta ctaatacatt
     1921 ttgatttcta gtagtcatta ttgctgttct agcacctggt tcaattacac caatcactgg
     1981 gattgataag gtcttttgta aatattctaa agctacagca gttgctgtat tacaagcaat
     2041 cacgagcatt tttatatcaa attccattaa tttacgagcg atttcaactg tatattgttt
     2101 tacttgttct cctggtcttg gcccatatgg acatcgtcca atatcaccta agtaataaat
     2161 cgtctcattt ggcaactgac gcataatttc tttagctact gtcaaacctc cgacaccaga
     2221 gtctattaca cctattggtt tattcatatt cggtcatcct tacttttaat ttaattttta
     2281 gtttatcata actaagcatt ggattttagt attatgcact gtgtttacca tttttgtcat
     2341 tataatattt attttaaatc agcccactat catattgtca tgtaatctgc ttattaaaaa
     2401 aatcccttcc aagttattgt gtatctccat tcaatttaat tttgaaagga acataacatt
     2461 ttaactcaaa agggattaat ttttattcta cttcatggtc tgaaccaaag aatgatttaa
     2521 acatgtggaa tgttgtttct ctgttcattg ctgcaatgga tgttgttaat ggaatacctt
     2581 tagggcaagc attaacacag ttttgtgaat taccacactg ctgtaagcca ccagtaccca
     2641 ttaatgcatt taaacgttca tctttagtca tagatcctgt tgggtgcaaa ttaaacaaac
     2701 gaacttgcga gattgcttgt gcaccaacga atttattatt ttcagtaaca ttaggacaaa
     2761 cctctaaaca tacaccacat gtcatacatt tagataattc ataagctgtt tgacgttttt
     2821 tctctggcat acgtggtccc ggacctaaat catacgttcc atcaattggg atccatgctt
     2881 tcatacgttt taagttatcg aacattctag aacgatcaac ttgtaagtca cggataactg
     2941 ggaaagtatt cattggctct aaacgaatag gttgttctaa ttgatcaaca atcgcagaac
     3001 aagattgtct tgcacgacca ttgataacca tagaacatgc tccacatact tcttctaagc
     3061 agttcatatc ccagacaaca ggtgttgttt tttcaccttt aatattaact gggttacgtc
     3121 taatttccat taaacaagca atgacgttta aattttcacg atatggaatt tcaaatgttt
     3181 cttcataagg cttagaatca cttgtatctt gtcgtttaat aattaatttt actgtttttt
     3241 gtttcggttt agattgtgtt tcatgttgtg gagtgttttt cactgattgt tcagtcatta
     3301 ttttttaccc cctttagact tacttgtgta atcacgttta cgaggtggta ttaaactcac
     3361 atcgacgtca tcataagtaa actgtggttt ttcaaatgcg ccttggaatg aggccattgt
     3421 cgtttttaac cactcttcat cattacgctc tgggaattct ggtttataat gggcaccgcg
     3481 tgattcgtta cggttatatg caccaatcgt aataacacgt gcaagtacta acatgttcca
     3541 tagttgacgg gtaaagaata ccgcttggtt actccaagtt tgagtatctt ccatatcaat
     3601 atcttcataa cgtttcatca attcaacaat ctttttatct gtttctaaca gtttttcatt
     3661 ttcacgaaca acagttacat ttgctgtcat aatttcacca agttcacggt gtaatttata
     3721 ggcattttct gtaccgcgca tagctaataa tttatcaaaa cgttcttgct cttcagcttt
     3781 acgtttttca aaaatacttt cgtccatatc agtatatgat cgatcaatat ttgaaatata
     3841 atcaatcgcg tttggacctg ctactgtacc accataaatc gctgataaca atgaattggc
     3901 acctaagcgg ttaccaccat gttgagagaa gtcacattct ccagctgcaa ataacccttt
     3961 aatatttgtc atttgatcat aatctacata tagaccaccc attgaatagt gaacagctgg
     4021 gaaaatcttc attggtactt tgcgtgggtc atcaccagtg aatttttcat aaatttcaat
     4081 gataccacct agttttacat ctaactcatg tggatcttta tgtgacaaat caagatatac
     4141 catgttttcg ccatttatac ctaatttttg gttaatacat acatcgaaaa tttcacgcgt
     4201 tgcgatatca cgaggtacta agttaccata atcaggatat ttctcttcta agaagtacca
     4261 aggcttacca tctttatatg tccaaattcg tccaccttca ccacgtgctg attcactcat
     4321 tagtcgcagt ttatcatcac cagggattgc agtaggatga atttgaatga actcaccatt
     4381 agcataaata gcgccttgtt ggtaaacaat ggaagccgct gatcctgtat taatcattga
     4441 gtttgttgtt ttaccgaaaa taataccagg gccacccgtt gccataataa ctgcatctga
     4501 accaaatgtt tcaatctcag cagttgtcat attttgtgca acgatacctc ttgcactatc
     4561 atcgtcacct ttaactatgc caagaaattc ccatccttca tacttcgtaa ctaatccatc
     4621 tacttcatat gcacgaactt gttcatccaa tgcatataat aattgttgtc cagttgttgc
     4681 ccctgcatat gctgttctgt ggtgtaatgt accaccgaaa cgtctaaaat ctaatagacc
     4741 ttcatttgtt ctattgaaca ttacacccat acggtctaat aaatgaataa ttttaggtgc
     4801 tgcctctgtc atcgctttaa caggtggttg gtttgcaagg aagtcgccac catacactgt
     4861 atcatcaaag tgaatccaag gagaatcgcc ttccccttta gtattgaccg caccattaat
     4921 gccaccttgg gcacaaacag agtgcgaacg ctttactggt acaactgaga acaaatctac
     4981 atgtgcacct ttttctgccg ctttaattgt tgacattaag cccgctaggc cacctccgac
     5041 aacaataaga tgtttctctg ccataaaaat ttcactcccc taaattttca atctatattt
     5101 gttaaatgcg atgtattaca taaaggcaat aattgcagta acaccaatat acgaaataac
     5161 taaaaatacg attaatgaaa cccatgtaaa tactcgttgt gattttggag attgaagtcc
     5221 accccaagta actaagaatg accataagcc atttgcaaag tggaacacaa cagcaataat
     5281 acaaataata taaaatattg cccatccagg atgttgcaat gtttcgtgca ttaaatcgta
     5341 attcacttct ttgccgtaaa atgctttttg taaacgtgtt tgccataaat ggataccaat
     5401 aaagataaat gttaagatac cactcactct ttggaagaag aacatccagt ttctaaaaat
     5461 cgagtaatgt ccaacatttt cttttgctgt aaatgcaatg tgtataccaa acaaaccgtg
     5521 atataacaac ggaatgtata taaataaaaa ttctacaata attagaaatg gtaatgattc
     5581 cataaagtta gatgccttat taaacgcttc agcaccttgt gttgcttggt gattcactaa
     5641 taaatgaacg accaaaaatg cacctattgg gataatacct aataacgagt gaatacgtct
     5701 tagataaaat tcattttttg attgagccaa aaggagtccc ccctgtgaac gaatatttaa
     5761 tttattgagc tatttatatt aaacgtacgc ttaaccccct aaagtgatat cgtttctagc
     5821 gttgtcatta tcattaagcg aaacatttta aagacaaata cacactgtac gatcaccaaa
     5881 ctgcatgtcg aacaatgtaa catttggatt cgatatttaa aattgcttgt gatgataaac
     5941 tttctcattt agaaaacgct tccacgtaca ttcaaaaaaa taactttgtt aaccatattg
     6001 taacattatt tcatatattt tggggcatga gaatgattct cacgcccagt aatttattta
     6061 tgcaattgtt catgtaggtt ctttgcgacg ttttcaggaa tacctatatt tttaaaatct
     6121 tcaagtgtag cttccttcat tttcttgatt gaaccgaatg aacgcaataa taatgtttta
     6181 cgtttgttac cgataccatc tatatcatca agtattgatt tcaagcctgt cttttgacgt
     6241 gtttgtctat gaaatgtgat tgcgaatctg tgaacctcat cttggatacg atgcaacaaa
     6301 taaaatgcct ggctattttt cttcagtggt acaatttctg cactagcgcc atataataat
     6361 tcagatgttt ggtgtttatc atttttctgc aaacctgcaa cagggatatc aagacctaat
     6421 tcgttttgta gcacatcaat aaccccgttc atatgtcctt taccaccatc gactattatt
     6481 aaatcaggta atggtaatcc ttcgtttaaa acgcgagaat atcgtcgtct tactacttct
     6541 ctcattgatt tgtaatcatc tggaccttta accgttttga ttttatactt tctataattt
     6601 ttcttatctg gtttaccgtc gacaaatgta accattgctg acactggatc cacaccttga
     6661 atattagaat tatcgaatgc ttcaattcta attggtgttt gaattcccat ttgtgttcca
     6721 agttcttcaa tagctttaat cgttcttgac tcatcacgtg atattaattc aaatttatta
     6781 tttaaggata ctttagcgtt atgtgcagct aggtcaacca tatctttttt gggacctcgc
     6841 gcgggttgaa cgattttagt gtccacaaca gattgaatca tttctttatc caaattacgt
     6901 ggtacatgaa cttccttagg taaaatatgt tggtttaagc tataaaattg tccaataaat
     6961 gtataaaatt cttcttcttc tgtttgctgt aatggaatca tcgttgtatc tcgctttatc
     7021 atattacctt gtcgtataaa gaaaacttgg atacacatcc atcctttatc aacacaataa
     7081 ccaaagacat cacgaatcgt tttatctgat gacataattt tttgtttgtt tgtcagattt
     7141 tgaatatgtt gaattaaatc tctatattct ttagcccgtt caaaatcaag tgattcactt
     7201 gcagttaaca ttcgctcttc taaacttttt aaaattgttt tgtcttcccc attcagaaaa
     7261 tcagtaattt ccttcgtcat ttgtgcgtat ttactcaaat caacgtcata tacacatggt
     7321 cctaaacatt gtccaatatg gtaataaaga cataatttat ctggcatctt atcacatttg
     7381 cgatatggat atattctgtc taataacttt ttagtttctt gagcagaata tgcattcgga
     7441 tacggtccga aatatttgcc agtaccttgt tttacagttc tcgtcactag tagtctagga
     7501 tatttctcct tcgtaatttt aataaatgga taacttttat catcctttaa taatatatta
     7561 tatcttggtt gatattgttt tatcagattc aattccagta aaagtgactc tgtttcactt
     7621 gacgtgacaa taaactcaaa acgacgtatt tcaccaacca gtctcgttgt tttagcatca
     7681 tgagcacccg taaaatatga tctcaatcga tttcttagct ttttagcttt gccaacatat
     7741 atcacttgat cattacgatc tttcattaaa tagcagcctg gttccatagg tacgacattt
     7801 aatttatttt taattcgttg cttatagtct tccaaaaata tccccccttc taattttaaa
     7861 atgaatgatg ctacaaaagt gcttcaaatt tatcaccact aattctagct cataatcaac
     7921 atcttcgagt atgacttttc atgcacatca tgtaatattg aacatggttt tatcaaacca
     7981 actgctacaa atctatcagc atgctagatt atcatttaac gttcatagat ttattaatcc
     8041 caccattctc aagtcgtaaa caatatgatg acttatcatt caacttttta tattaaaaac
     8101 acaaacaaaa actggagcaa ttcattaaag aaatgcccca gtcgtcattg gttataactt
     8161 ataaatgttt atctaaaact tcagctaagt tttcttttgg ttggaaacca acaactttat
     8221 caactggttg accgtcttta aagacgatta atgttggaat actcatcact tcatatttag
     8281 ctgcagttga tggattttca tcaacatcta attttaaaat gtcagcttta ccttcatagt
     8341 cagctgctaa ttcttctaat accggagcga tcattttaca tggaccacac catgttgccc
     8401 aaaaatctac tagttgtaca ccagattcta cttttgaatc aaaatctgca tctgttactt
     8461 ttacgattgc cataaatgcc aatcctcctt aattattatt atgatgttca aattatatca
     8521 gataacatgc ttcatgtatt tctatttgct caatttttat gcttatttca aataacatgt
     8581 ttgaaatgta ttcttgttgc tttacattat tttaactgca gctatttaat ttatcaaatt
     8641 ataatttatt ttagtgttgc aacggtaacg ccaaatccac cttcgcttgg cataccacct
     8701 ctaaagtcac taacactttt atgcttcttc aaatgttgtt gtacaccttt ttgaagtgca
     8761 cctgtacctt taccatgaat gatataaact tgttcgtaat tacttaaaac ggcttgatct
     8821 aaatattgat ctagttcaat taaagcatcc tcataacgat agcctcgtaa gtcaagttca
     8881 gttttaattg tttgacgatt ttgacgtgta accattttcg ttggcttaac tttttctttt
     8941 tgttttttct ctaaatcttc aataggtaac ttcattttaa taattcccat ttgaacaatt
     9001 gcttcttcat cattgacaat ttctaaaact tcaccctttt gaccgtaaga taatactttt
     9061 acttcatcac cagcaacaat tttatcgtat ttttgctttt gtacattttg ctttatagat
     9121 ttcgcttcat aatgatcatc taatcgtttc ttcttatcaa tcaattcatg ttctttaaca
     9181 tctgcacctt tttgttcacg caattgtctt aagtctttaa taatatcgtc agcttctttt
     9241 gttgcagctt taatcttctg atttgctttt tctttcgctt cctctattag agacttttca
     9301 taattttgga attgttgata ctgcttagat aaatcatcat gcacttgctc cgcttctttt
     9361 acaagacggt ccagttctaa cctctgtgtc tctacacgtt tataattacg ctctaatgat
     9421 tcaatcattt catttatttc tttttcatca gtaccaatca tcgtcttagc cttattaata
     9481 atattcaaac taagacctaa ctttttagaa atgtcaaaag catttgaacg acccggcaca
     9541 cccattaata acttatacgt tggactcaaa gtatctacat caaattctac actcgcattc
     9601 ataacgcctt ctcgattata actatatgct ttaagttcag gatagtgcgt cgttgccatt
     9661 actagagaac caatttttct aacatgatct aaaatgctca ttgctaatgc agcaccttca
     9721 cttggatctg tacctgcacc taattcatca aataaaacta aactatgttt gtctgcatgc
     9781 tttaaaattt caactatatt cgtcatatga gatgaaaaag ttgataatga ttgttctatt
     9841 gattgttcat ctccgatatc gcaatataca tttttaaata cacttaactg actaccatca
     9901 agtgtgggaa tcaacaatcc tgattgagcc ataacaataa ttaaacctaa tgtttttaat
     9961 gttacagttt taccacctgt attcggtcct gtaataatta ccgtttcaat atcttccata
    10021 aattcgatgg tattagctac aacagtctca cgatttaata atggatggta tgctttaggt
    10081 aaatatacag tacgttcctc tttaaatatc ggctttgttc ctttaatact tctactatat
    10141 ctcgcttttg cgattaaaaa aactaactgc cccatgactt gttctgccac aagtagtgca
    10201 tctttgtccg cagccacata accagttagt tgcgttaaaa cgcgttcttt ttcaattgct
    10261 tcgtcatgac gtaatcgact aatttgatta ttcatttcaa caactgatga tggctcaata
    10321 tacaatgttt gtcctgaagc agattgatca tgtacaatcc cattaaaatc ttgtcgatat
    10381 tcagctttga caggtataac gtttctttca ttcctaactg ttacaatagc atctgataat
    10441 tttttctgat ttgcttggct tttaacaata cggtccaaat tttgtctaat acgttgattc
    10501 gtgctagaaa ttttacttct aatcccttgc aattcataac tcgcattatc atataaatca
    10561 tacgtatcgc atgtttcatt tatttgatga aaaagatcag ttaacacagg taattgattc
    10621 atcttgtcat ctaatattgg gtatttaaca ccttcatctt cttcaaccaa ttgattataa
    10681 aatgtcttaa attgattttg tacttgaatt aatcttttta tcaagttaag ctctgataca
    10741 tttaaaacgc cgccaatatc agcgcgatga atgaatgctg atactttaga taagccactc
    10801 aagcttggta aacgatgctt attatagatt tgagcaatct catccgtttc ttccatttga
    10861 aaaacaaccg tttcaaaatt agtagctggc atcatttgat tgaccttttc caagcctaag
    10921 tcactaatag tttcattggc aacgagtgat tttatttttt caaattctaa gacgtctaat
    10981 gttttttgtc tcataaaatc cctctatttc ttaagtttta tattattttc aataaagtct
    11041 ttaaaagctt cacgcgataa tgcgttaatc actctatctt ttgttacaaa tcctttttgc
    11101 gcagttgcta cgccataatt cataaaatct aaatgatttg tatgatgcgc atcagtgtta
    11161 atagttaatt tcacatttgg atatttacga acgatttcag cgttcagatc cagtcgatgt
    11221 ggattggcat taatttctaa tactgtattc gtttcttcag ctaatgccat taattgttca
    11281 atattcggtt tataaccatc tcttctacct ataatacgcc ctgttggatg cgctatatgt
    11341 cgcacgtatg gattgcgaca tgcattagct aatcgttcca taatttgttc ttctgattga
    11401 ttaaagcttt gatgaatagc tccaattaca taatcaagtt gtgctaaaat ttcatcatca
    11461 taatccagcg agccatcagg taatatatcc atttctgtac ctgaataaat atcaatttca
    11521 ctatattctt tatctaaagc cttaatttct tcgttttgtc ttaaaagtct ttccacttgt
    11581 aagccattag caacacgtaa actttgtgaa tgatcagtaa ttaccatgaa tttataacct
    11641 tttgcgatat ttgcttctac catgtctcga atagaaaacg caccatcact atacgttgta
    11701 tgcatatgaa tatcaccatt aatatcatct aatgtaatga tattacttag atctttatca
    11761 aattcgctac catcttctcg catagcaggt ggtataaaat tcacattaaa atgttcatat
    11821 atcttggctt cactatcata ttgaattaat gtaccatcag cttgttcaat tccatattca
    11881 cttacttttt catcacgtgc tttagcaagt tgtcgaattc ttatattatg ttcttttgac
    11941 ccagtaaaat gttgcaatgt atgataaaaa gcccttggtt caattaatcg aaaatcgaca
    12001 ccaatcgttt catcatcata cgctaattct aatgaaactt ttgtgttccc cactgcaact
    12061 tcttttactt tattgggaat atttaataat tgctgctgca ctgcttttgg gttatcggta
    12121 cttattatga aatctaaatc tttgctcatt tctttaaaac gacggaagct tcctgcagat
    12181 gaatattgat cgatataatt taatgtatct atataatcaa tgatttcttg attaagtctt
    12241 ctcatttgat caattggata tctatctttc ttagcaccaa gttgtttcac agcttctaat
    12301 atgttttgtt ccgttttctt agcaaatccg cttaattcac taacttttcc attttcacaa
    12361 gcaacttgaa gtgacgcttt atcaacaata ttcaactctt tatatagctt agcaattttc
    12421 ttgcttccaa gtccttgaat tttcaaaagt ggaataagac cttccggaac ttcttcctgt
    12481 aattgctgta aatactgaga ttcaccggtc tcacggtaat cattgattac ttctgcaaca
    12541 cctttaccaa tgccttttaa ctccgttaca tcagatattt catctaatgg tcgttcatct
    12601 aattcaagac tttgagctgc ttttcgatac gctgatattt taaaagtatt ttcccctttt
    12661 aattccatat aagtagcaat ttgttctaat agtttgataa catctttttt tgtcataata
    12721 acactccata aaaagaagac caggacgtat cattaatata tacctttgtc ctgacctctt
    12781 atgataattt ataaattaat ggtgaaagcc gataaatatg ggatgtgaaa aatcaatgaa
    12841 tgactcacaa tagatatttt aagctgatgt tgtaatgctt cgttaggata taatgccaat
    12901 agatataacg taaagtgtaa gacaattatc gtcataaaca caccaactat cattcccatt
    12961 gcacgactga aaatatgaat gttttgatac gctattattt tatcaaaagt tacgataatt
    13021 agatataaaa tgaacttaca aaacaatgta atcattaaaa aagctacaat cgcttcaaat
    13081 cgattttgta gatgattaaa atgaaacgca aaagttgtat taaatgctgt tgttttagga
    13141 tatggaataa atacaattaa tctttctaca atagatttgt aaaattgact ggcaatccac
    13201 aatgatacaa tcgttgcact caaatgtatc atagataacc aaaaacctcg tctgaatcca
    13261 acgatgacaa aatacacaaa gaaaatgatt atgataaaat caatgaccat ttattgctca
    13321 cgctgctgca atttgtgaat ttgttgtttc aaacgtcgat tttcttcttc tagtagtact
    13381 ttttcatgca taatattcac agcagttagt attgcttttc ttgaagtatc taaacctgct
    13441 gctttatacc ctaattcttt tattttatca tcaactaaat gtgctacata tcgtatgtgc
    13501 tctgggttat cttccccaac aattgtaaaa agctgatcat taattgatac atttaccttg
    13561 tttttaaact gtgccattta taatttctcc tgatcctttt tttaaaatct aaattcacgt
    13621 tataaaatat gactggatag tttgtctgaa tttgatacta atattgttat attgcaatta
    13681 tgataaaaca acaacacaat ctctatagat gacttaatgt tctttttata atgaaataat
    13741 gtaaagaatt ttctattcaa tactttatca tgtttaaatt gtgtcactat aacattttca
    13801 taaacattat acatgaccac tatgtatttt gtaagtatcc gcaattaatt ctttacaaca
    13861 tacataaatg tttctgacgt tattatcatt tatgatatga ttatttttgc taatgataat
    13921 gaaattatat gaaaggattt acacaaatgg cgaatatcgt ttttaaattg tcggataaag
    13981 acataacgac attaatgtca cgcattactt ttgatactga gaatttacct caaggtatga
    14041 aagcacgtgc aaagtatcaa aatacaactg taaatattta ccaatcaggt aaagtcatgt
    14101 ttcaaggtaa tcatgcagaa gctgtgtcta aagaacttct accgcagcat tctcaattga
    14161 atactaataa aactaaaaag aaaaatatgg ctaattcttc tcttgaacaa actttaatgt
    14221 atgatcaatt taactgtatt ggtagtgatg aggctggaag tggcgattac tttggtcctt
    14281 tgactgtgtg tgctgctttt gttaccaaag agcatgttcc tattttaaaa actttgggtg
    14341 tagatgattc aaaaaaatta accgatacca aaattgtaga acttgctgag caattggttg
    14401 ctttcatccc acattcacta ttaacactac acaatgataa atataatatt cagcaagcaa
    14461 aaggctggac tcaagtaaaa atgaaagctg ttcttcataa tgaagccatc aaaaatgtac
    14521 ttgaaaaaat tgattcgtct caattagact atatcgtcat tgatcagttc gcaaaacgcg
    14581 aagtgtatag ccattacgca ttatccgata taccacttcc aaagaaaaca aaatttgaaa
    14641 caaaaggaga atcgaagtca ttagccattg cagtggcaag cataatttct agatacgcat
    14701 ttattacata tatggatcaa atatcaaaat atattaatat gacgattcct aaaggtgccg
    14761 gtgctaaagt cgatgtaatt gccgcaaaaa ttataaaaaa atacggtctt tcacgtttag
    14821 ataccatttc aaaaaagcat tttaaaaatc gtgaaaaagc ccaaaaaatt ttaaagcctc
    14881 tttgattttt aatcattaga ggcttttatg cagttgtgcg aagcatcatc tgtattccgc
    14941 tgttcctctt tgatttataa aatgaattgg atcctaatag gtgatcctat taggtaagtc
    15001 atactaattt gctttgctca taagtatgac tttatatgca gtgatgcgaa gcatcatctg
    15061 taatccactg tgcctctatg atttataaaa tcaagataaa ttggatccta ataggaaatc
    15121 ctattaggta agtcatacta atttgctttg ctcataagta tgactttata tgcagttgtg
    15181 agaagcatca tctgtattcc gctgtgcctc tttgattttt aaatgaatcg aaacctaatt
    15241 ggtgaatcca aaaataaaac cccatgttaa ggatatcaat ctacttcaga tatccttata
    15301 catggggttt aaatcattat ctaataacag caccttgttc aattaatgct gcttcaattt
    15361 ccgcttgcac ttttgaaacg cgctcatctg tcaatgtttc ttctgtgtct aaataattta
    15421 aacgtattgc aattgatttt ttaccttttt ctaaatgttc gccctgatat acatcaaaga
    15481 caagtgtatc ttttaatata ttgccaccgt gtgcatgaat cgttgataat aaatcagctg
    15541 ctggaatatt ttgatctact tctaatgcaa tgtcacgaga catgcctggg aatctcggaa
    15601 ttggctggta attaatgtaa cctaccgaca cagccattaa tgcatcaaaa ttcaactcaa
    15661 aaacatacgt acgttttaaa tcattatcag ctgctaatgt tggatgtaat tcaccaataa
    15721 aaccaacaac tttattctct aataagattt cagcagtacg acctggatgt aatccatcaa
    15781 tatcagcacg acgataacta aattcaaggt ttaacttttc agatactcga tccacgacac
    15841 cttttgctaa atagaaatca accgtttctt tcttaccttg ccattgattg actacataat
    15901 ctccagttaa aataccactt aaatattcaa cttgatctgg tagttcacct tctccattag
    15961 caaagaagac attgccgatt tcaaataatt ttacatcttt atttttgcgt gccacattat
    16021 atgatgctgc ttcgattaaa tgtggtaata aactttgacg taatgacgca tgcgcttcac
    16081 tcattggcat caataaatca attgtttgac gctgttgcat cgaaaatgca gtagcatcct
    16141 ctttagaaac taacgaatac gtaatagctt gatctaatcc agcaccttct aacacttctt
    16201 taaccattct agttttatat tggcgatcag ttagctgacc actagtaact ttatcgaaga
    16261 caggtaacgt tgatggaata tcgtcgtatc catatatacg tgcaacttct tcaattaaat
    16321 cttctttaat tgtaatatct ttacgacgtg atggtactag cactgtaata acatcatcat
    16381 ttatttctgt atcaaaccct agttgattaa aaatagtaac aatatcattt tgtgacaaat
    16441 caaatccaat agtgcgatta attttatcag cagtgatgtc gattggtgta ataaatgcac
    16501 caagttctcc tgaagacact ctatctttta gcacttttcc gtttgcataa gtttgtaata
    16561 aataacatgc acggtcgact gcttcatcta caaattcagt agctattcct ttttcaaaac
    16621 gactagatga ttcactgcgt aaatttaaac gtcttgatgt atgacgaatt gaaactggat
    16681 caaaaatagc accttcaatc actatatttg atgtttgttc tttaacttct gaaaaatcgc
    16741 cacccataac accagctaat gcaattggag tttgtccatt agtaatgaca atatcgctcg
    16801 ttaataattc acgttctgta tcatctaatg ttgtcatttt ttcgccttca ttagcttgac
    16861 gaacaacaat ttgttgtgaa ccaatcgcat cttgatcaaa catgtgcaat ggttgaccgt
    16921 attctaataa cacataattt gaaatgtcaa caacattatt aataggacgt atacccgctt
    16981 ttattaagcg tgcttgcatc caaattggcg aaggttcaat tgtcacgtcg tgaacaacac
    17041 gtgcactata atatggtact ttatcttcat tttcaattgt cacagtcaat tcatcatttg
    17101 cagataactc aagctcattt gatgttgtct ctggcttagt catttttgta ttatataatg
    17161 ctgcaacttc ataagcagta cctatcatac ttaaagcatc tgcacgattc ggcgttaaat
    17221 caaattccat tacttgatca tctaaatata aagcttgtaa ggcatctgtt cctggaactt
    17281 gggattcact aaaaacataa atgcctgatt caaaactttt cggtatatag ttacttgaaa
    17341 taccaatttc ttgtaacgaa caaatcatac cttctgaacg ttcaccgcgt aatttggcac
    17401 gcttaatttt aataccacca ggcaatctgc cacctacttt agcaacaatg acatattgtc
    17461 ctgcatcaac gttcggtgca ccacatacga tttgtacagg ttcgtcttct ccgatatcaa
    17521 cttggcaaac atttaattta tcggcatcag gatgtttctc ttttgactta acgaaaccga
    17581 caactaaatt tttgatatct tttgtgtagt caattaaatc atccacttca atacctgtgc
    17641 gcgtaatacg ttctgccaaa tcacttacag aatcatcgat tgttacatat tctttcaacc
    17701 attcatttga tatcaacatt acatgtcacc tctatcttct accgctttaa attgatctaa
    17761 aaatctcaca tcattagtat agaaatgacg aatatcttct ataccatatt tcaacattgc
    17821 aatacggtct ggtcccatac caaatgcaaa tccagagtac tctgaagaat caaaaccagc
    17881 catttctaat acattaggat gtaccattcc agcacctaaa atttcaatcc atcctgtgtg
    17941 tttacacaca ttacaacctt ttcctttaca tttaaaacat gacacatcaa cttctacaga
    18001 aggttcagtg aatgggaagt aacttggacg taaacgaatt tcacgatcag caccaaataa
    18061 tttcttagct aacaattcta aagtgccttt caaatcactc attttaacgt ttttatcaac
    18121 aactaatcct tcgatttgtg taaattgatg actatgtgtc gcatcatcag agtcacgacg
    18181 atacacttta ccagggcaaa taattttaac tggaccttga ccatgacgtg attccatcgt
    18241 acgtgcctgc actggtgatg tatgcgtacg taataaaatt tcatccgtaa tatagaaact
    18301 atcttgcata tcacgtgcag ggtgtgattt aggtaaattc agcatttcga agttataatg
    18361 atcttgttca acttcatatc cattcacaat ttcataacct aaacctaaga ataagtcttc
    18421 aatttcttct attgtacgtg ttaatggatg ctttgaaccg atttcaatat gacgacctgg
    18481 taatgataca tcaattgttt cttcagccaa ttgcttattt aatttttctt taactaacat
    18541 ctgttgtctt tcatctaatt cattttgaat tgtttgacgc aattcattca ctttttgacc
    18601 aaacgcaggt ttctcttcat tcggcaaatc cttcatcaat ttcattagtc cgctaactga
    18661 ccctttttta cctaagtatt tcactttaac ttcttgcagt gcacgttcat catttgcttc
    18721 attaatatct acaagcgctt gttgttttaa ctctgacatt gtttgttgtt cagacatatg
    18781 tcactgacct ccctattcga actattcatt ttctattgat catatttgtt gtttaactaa
    18841 aaaatgactt tcatcctctt aaaaataagg gacgaaagtc atttccgtgg taccacccaa
    18901 atttatgcct aagcatacac ttgtttaata ttgataacgg attgtctttc cgacttaaat
    18961 ctctttaagt aaccaaaaag gtgaaaagac tttaaataat tggaataact tgcagtcacg
    19021 attattctcc ctaaaacaat tatttatata aatgtccatg cctttttatt ggtttatatt
    19081 caattgtgta tgcattcacg tagaaatgtt ctgttaaaca attaattata attatatcgg
    19141 aaaactttca acacggtcaa cctttcaaat gataaagtaa aatactacct gcaatcgcta
    19201 catttaaact ttcagcttta ccataaatag gtatgattaa attttgtgta gtatgtgcta
    19261 ataattcagg attaacacct tctccctcat tacctaatag taatgcaaaa gaatcacttg
    19321 aagtaacttc tttgtatgcc actgcgtttt caagtgctgt accataaaca ggaccattaa
    19381 attgagtaat aaacgtatcg agatcttgtg tcataactgg caaatggaaa acactacctt
    19441 gactcgctcg caacacttta tcttgataag gatctgtcgt acccttctcc attattacag
    19501 catccattcc agcagcatcc gcagttctaa ttaatgtgcc taaatttcca ggatcttgaa
    19561 cacgatcgat gagcaatact tgttgtgctt tagaaatatc ataatgcggc ttctcaatga
    19621 ttgcgaaaaa cccttgtggt gtcactgtac ctgataaaga ttcagcaact ttcatgttta
    19681 tttcaaaaac ttcttgcgcg tatgcgataa tttgctgatc taatcttgcc ggttcaattg
    19741 caaataattg tgtaattaca attccacttt gataagcttc ttcaattaaa tgcacacctt
    19801 caattaaagc taatccagtt ttatccctct cacgtttctt ttttagcttg ttcgcttgtt
    19861 taattctatt attttgtgca gaagtaattt gttccattga tag
//
LOCUS       DANBWH010000024        19749 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.024, whole genome shotgun sequence.
ACCESSION   DANBWH010000024 DANBWH010000000
VERSION     DANBWH010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19749
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.024"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..216
                     /gene="lspA"
                     /locus_tag="PPY92_000597"
     CDS             <1..216
                     /gene="lspA"
                     /locus_tag="PPY92_000597"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDJ2624620.1"
                     /translation="QVAISLLFAGALGNFIDRILTGEVVDFIDTNIFGYDFPIFNIAD
                     SSLTIGVILIIIALLKDTSNKKEKEVK"
     gene            216..1133
                     /locus_tag="PPY92_000598"
     CDS             216..1133
                     /locus_tag="PPY92_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDJ2624621.1"
                     /translation="METYEFNITDKEQTGMRVDKLLPELNNDWSRNQIQDWIKAGLVV
                     ANDKVVKSNYKVKLNDHIVVTEKEVVEADILPENLNLDIYYEDDDVAVVYKPKGMVVH
                     PSPGHYTNTLVNGLMYQIKNLSGINGEIRPGIVHRIDMDTSGLLMVAKNDIAHRGLVE
                     QLMDKSVKRKYIALVHGNIPHDYGTIDAPIGRNKNDRQSMAVVDDGKEAVTHFNVLEH
                     FKDYTLVECQLETGRTHQIRVHMKYIGFPLVGDPKYGPKKTLDIGGQALHAGLIGFEH
                     PVTGEYIERHAELPQDFEDLLDTIRKRDA"
     gene            1533..2060
                     /gene="pyrR"
                     /locus_tag="PPY92_000599"
     CDS             1533..2060
                     /gene="pyrR"
                     /locus_tag="PPY92_000599"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDJ2624622.1"
                     /translation="MSERIIMDDAAIQRTVTRIAHEILEYNKGTDNLILLGIKTRGEY
                     LANRIQDKIHQIEQQRIPTGTIDITYFRDDIEHMSSLTTKDAIDIDTDITDKVVIIID
                     DVLYTGRTVRASLDAILLNARPIKIGLAALVDRGHRELPIRADFVGKNIPTSKEETVS
                     VYLEEMDQRNAVIIK"
     gene            2278..3585
                     /locus_tag="PPY92_000600"
     CDS             2278..3585
                     /locus_tag="PPY92_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDJ2624623.1"
                     /translation="MQNDEMFERTVKPVLDVNEKPQPAQWAFLSLQHLFAMFGATVLV
                     PFLTGLPISAALLASGIGTLLYILITKAQIPAYLGSSFAFITPIITGLSTHSLGDMLV
                     ALFMSGVMYVIIGILIKLSGTAWLMKLLPPVVVGPVIMVIGLSLAPTAVNMAMYENPG
                     DMKGYNISFLIVAMITLLVTIVVQGFFKGFLSLIPVLVGIIVGYVVAIFMGIVKFDAI
                     MSAKWIDFPHIYLPFKDYVPSFHLGLVLVMIPIVFVTVSEHIGHQMVLNKIVGRNFFE
                     KPGLDKSIIGDGVSTMFASIIGGPPSTTYGENIGVLAITRIYSIYVIGGAAVIAIVLA
                     FIGKFTALISSIPTPVMGGVSILLFGIIAASGLRMLVESKVDFANNRNLVIASVILVV
                     GIGNLVFNLKEIGINLQIEGMALAALSGIILNLILPKEKKQNN"
     gene            3613..4494
                     /locus_tag="PPY92_000601"
     CDS             3613..4494
                     /locus_tag="PPY92_000601"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HDJ2624624.1"
                     /translation="MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLF
                     FENSTRTKCSFEMAELKLGLKTISFETSTSSVSKGESLYDTCKTLESIGCDLLVIRHP
                     FNNYYEKLANINIPIANAGDGSGQHPTQSLLDLMTIYEEYGYFEGLNVLICGDIKNSR
                     VARSNYHSLKALGANVMFNSPNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGL
                     AEETRFAADDYHQKHGLNEVRYNKLQEHAIVMHPAPVNRGVEIQSDLVEASKSRIFKQ
                     MENGVYLRMAVIDELLK"
     gene            4512..5786
                     /locus_tag="PPY92_000602"
     CDS             4512..5786
                     /locus_tag="PPY92_000602"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDJ2624625.1"
                     /translation="MKLIKNGKVLQNGELQQADILIDGKVIKQIAPAIEPSNGVDIID
                     AKGHFVSPGFVDVHVHLREPGGEYKETIETGTKAAARGGFTTVCPMPNTRPVPDSVEH
                     FEALQKLIDDNAQVRVLPYASITTRQLGKELVDFPALVKEGAFAFTDDGVGVQTASMM
                     YEGMIEAAKVNKAIVAHCEDNSLIYGGAMHEGKRSKELGIPGIPNICESVQIARDVLL
                     AEAAGCHYHVCHVSTKESVRVIRDAKRAGIHVTAEVTPHHLLLTEDDIPGNNAIYKMN
                     PPLRSTEDREALLEGLLDGTIDCIATDHAPHARDEKAQPMEKAPFGIVGSETAFPLLY
                     THFVKNGDWTLQQLVDYLTIKPCETFNLEYGTLKENGYADLTIIDLDSEQEIKGEDFL
                     SKADNTPFIGYKVYGNPILTMVEGEVKFEGDK"
     gene            5788..6888
                     /gene="carA"
                     /locus_tag="PPY92_000603"
     CDS             5788..6888
                     /gene="carA"
                     /locus_tag="PPY92_000603"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDJ2624626.1"
                     /translation="MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETIS
                     DPSYTGQIITFTYPLIGNYGINRDDFESLVPTLNGIVVKEASAHPSNFRQQKTLHDVL
                     ELHQIPGIAGVDTRSITRKIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVS
                     TKTPYVSTGKDLSVVLVDFGKKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVML
                     SNGPGNPEVVECAIPMIQGILGKIPFFGICLGHQLFALSQGASSFKMKFGHRGANHPV
                     KNLETGKVDITSQNHGYAIDIDSLKSTDLEVTHLALNDGTVEGLKHKTLPAFSVQYHP
                     EANPGPSDSNYLFDDFVAMMTNFKEKERHINA"
     gene            6881..10054
                     /gene="carB"
                     /locus_tag="PPY92_000604"
     CDS             6881..10054
                     /gene="carB"
                     /locus_tag="PPY92_000604"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDJ2624627.1"
                     /translation="MPKRNDIKTILVIGSGPIIIGQAAEFDYAGTQACLALKEEGYRV
                     ILVNSNPATIMTDKEIADKVYIEPLTHDFIARIIRKEQPDALLPTLGGQTGLNMAIQL
                     HESGVLQDNNVQLLGTELTSIQQAEDREMFRTLMNDLNVPVPESDIVNTVEQAFKFKE
                     QVGYPLIVRPAFTMGGTGGGICHNDEELHEIVSNGLHYSPATQCLLEKSIAGFKEIEY
                     EVMRDKNDNAIVVCNMENIDPVGIHTGDSIVVAPSQTLSDVEYQMLRDVSLKVIRALG
                     IEGGCNVQLALDPHSFDYYIIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     LNPITGTSYAAFEPTLDYVISKIPRFPFDKFEKGERELGTQMKATGEVMAIGRTYEES
                     LLKAIRSLEYGVHHLGLPNGESFDLDYIKERISHQDDERLFFIGEAIRRGTTLEEIHN
                     MTQIDYFFLHKFQNIIDIEHQLKEHQGDLEYLKYAKDYGFSDKTIAHRFNMTEEEVYQ
                     LRMENDIKPVYKMVDTCAAEFESSTPYYYGTYETENESIVTDKEKILVLGSGPIRIGQ
                     GVEFDYATVHAVWAIQKAGYEAIIVNNNPETVSTDFSISDKLYFEPLTEEDVMNIINL
                     EKPKGVVVQFGGQTAINLADKLAKHGVKILGTSLENLNRAEDRKEFEALLRKINVPQP
                     QGKSATSPEEALANAAEIGYPVVVRPSYVLGGRAMEIVDNDKELENYMTQAVKASPEH
                     PVLVDRYLTGKEIEVDAICDGETVIIPGIMEHIERAGVHSGDSIAVYPPQTLTEDELA
                     TLEDYTIKLAKGLNIIGLINIQFVIAHDGVYVLEVNPRSSRTVPFLSKITDIPMAQLA
                     MRAIIGEKLTDMGYQEGVQPYAEGVFVKAPVFSFNKLKNVDITLGPEMKSTGEVMGKD
                     TTLEKALFKGLTGSGVEVKDHGTVLMTVSDKDKEEVVKLAQRLNEVGYKILATSGTAN
                     KLAEYDIPAEVVGKIGGENDLLTRIQNGDVQIVINTMTKGKEVERDGFQIRRTTVENG
                     IPCLTSLDTANALTNVIESMTFTMRQM"
     gene            10164..10856
                     /gene="pyrF"
                     /locus_tag="PPY92_000605"
     CDS             10164..10856
                     /gene="pyrF"
                     /locus_tag="PPY92_000605"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDJ2624628.1"
                     /translation="MKDLPIIALDFESKEKVNQFLDLFDESLFVKVGMELFYQEGPQL
                     INEIKERGHDVFLDLKLHDIPNTVGKAMEGLAKLNVDLVNVHAAGGVKMMSEAIKGLR
                     KHNQHTKIIAVTQLTSTTEDMLRHEQNIQTSIEEAVLNYAKLANAAGLDGVVCSPLES
                     RMLTEKLGTSFLKVTPGIRPKGASQDDQHRITTPEEARQLGSTHIVVGRPITQSDNPV
                     ESYHKIKESWLV"
     gene            10856..11467
                     /locus_tag="PPY92_000606"
     CDS             10856..11467
                     /locus_tag="PPY92_000606"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDJ2624629.1"
                     /translation="MAKEIAKSLLDIEAVTLSPNDLYTWSSGIKSPIYCDNRVTLGYP
                     LVRGAIRDGLINLIKEHFPEVEVISGTATAGIPHAAFIAEKLKLPMNYVRSSNKSHGK
                     QNQIEGAKSEGKKVVVIEDLISTGGSSVTAVEALKQAGAEVLGVVAIFTYGLKKADDT
                     FSNIQLPFYTLSDYNELIEVAENEGKISSEDIQTLVEWRDNLA"
     gene            11497..11709
                     /locus_tag="PPY92_000607"
     CDS             11497..11709
                     /locus_tag="PPY92_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624630.1"
                     /translation="MNDKTSNDLYGKIKHCNEFINHSNDSNLSSSHDVDESSTKQKHI
                     KNKTTIDHNDDLFKHVKDILRKQGQI"
     gene            12146..12547
                     /locus_tag="PPY92_000608"
     CDS             12146..12547
                     /locus_tag="PPY92_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDJ2624631.1"
                     /translation="MDIPKITTFLMFNNKAEEAVKLYTSLFEDSEIITMAKYGENGPG
                     DHGTVQHSIFTLNGQVFMAIDANSGTELPMNPAISLFVTVKDTIEMERLFNGLKDEGA
                     ILMPKTNMPPYREFAWVQDKFGVSFQLALPE"
     gene            complement(12810..14507)
                     /locus_tag="PPY92_000609"
     CDS             complement(12810..14507)
                     /locus_tag="PPY92_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HDJ2624632.1"
                     /translation="MAYDGLFTKKMVESLQFLTTGRVHKINQPDNDTILMVVRQNRQN
                     HQLLLSIHPNFSRLQLTTKKYDNPFNPPMFARVFRKHLEGGIIESIKQIGNDRRIEID
                     IKSKDEIGDTIYRTVILEIMGKHSNLILVDENRKIIEGFKHLTPNTNHYRTVMPGFNY
                     EAPPTQHKINPYDITGAEVLKYIDFNAGNIAKQLLNQFEGFSPLITNEIVSRRQFMTS
                     STLPEAFDEVMAETKLPPTPIFHKNHETGKEDFYFIKLNQFNDDTVTYDSLNDLLDRF
                     YDARGERERVKQRANDLVRFVQQQLHKYQNKLAKLIEEYEQSKNKDTEQLYGELITAN
                     IYRIKQGDKEVTALNYYTNEEVVIPLNPTKSPSANAQYYYKQYNRMKTRERELQHQIQ
                     LTKDNIDYFSTIEQQLHHISVHDIDEIRDELAEQGFMKQRKNQTKKKKAQIQLQHYVS
                     TDGDDIYVGKNNKQNDYLTNKKAKKTHTWLHTKDIPGSHVVIFNDAPSDTTIKEAAML
                     AGYFSKAGNSGQIPVDYTLIKNVHKPSGAKPGFVTYDNQKTLYATPDYELIQKMKQL"
     gene            14782..15405
                     /gene="gmk"
                     /locus_tag="PPY92_000610"
     CDS             14782..15405
                     /gene="gmk"
                     /locus_tag="PPY92_000610"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDJ2624633.1"
                     /translation="MDNEKGLLIVLSGPSGVGKGTVRKRIFEDPSTSYKYSISMTTRQ
                     MREGEVDGVDYFFKTRDAFEALIKDDQFIEYAEYVGNYYGTPVQYVKDTMDEGHDVFL
                     EIEVEGAKQVRKKFPDALFIFLAPPSLDHLRERLVGRGTESNEKIQSRINEARKEVEM
                     MNLYDYVVVNDEVELAKNRIQCIVEAEHLKRERVEAKYRKMILEAKK"
     gene            15405..15623
                     /locus_tag="PPY92_000611"
     CDS             15405..15623
                     /locus_tag="PPY92_000611"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDJ2624634.1"
                     /translation="MLNPPLNQLTSQIKSKYLIATTAAKRAREIDEQPETELLSEYHS
                     FKPVGRALEEIADGKIRPVISSDYYGKE"
     gene            15839..17038
                     /gene="coaBC"
                     /locus_tag="PPY92_000612"
     CDS             15839..17038
                     /gene="coaBC"
                     /locus_tag="PPY92_000612"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDJ2624635.1"
                     /translation="MKKILLAVTGGIAAYKAIDLTSKLTQSGYEVRVMLTNHAQKFVT
                     PLAFQAISRNAVYTDTFIEENPSEIQHIALGDWADAIIVAPATANTIAKLSVGIADDL
                     VTSTLLATETPKFIAPAMNVHMYENKRTQQNINILKEDGYHFIEPGSGFLACGYVAKG
                     RMEEPLQIVSVIDAHFQNSNRLANSSFQDKRALVTAGPTIEVIDPVRFVSNRSSGKMG
                     YAIAEALRNRGAIVTLVAGPTTLEDPKDIEVIHVQSAEEMFEQVTSRFDEQDIVVKAA
                     AVSDYTPVDVLEHKMKKQDGDLSVSFKRTKDILKYLGEHKTSQYLIGFAAETEDIENY
                     AQQKLRKKNADVIISNNVGDMSIGFSSDDNELTMHFKNNEKVNIKKGKKVVLAAQILD
                     ELETRWQ"
     gene            17038..19446
                     /gene="priA"
                     /locus_tag="PPY92_000613"
     CDS             17038..19446
                     /gene="priA"
                     /locus_tag="PPY92_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDJ2624636.1"
                     /translation="MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRT
                     IQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTSELIALSEWMGSTHVIKRISMLE
                     VMLPSAIKAKYKKAFKMKDDIELPSALLQKFDKHGYYYYKDAQKNNDIQLLMKLLKDD
                     IVEEKTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFL
                     TDIEDMGFSKSSLDGLIKKGYVEKYDAVVERDPFKDRVFEQESKQQLTEDQYKAYEAI
                     KAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLVPEIALTPQMVLRFK
                     RRFGDDVAVLHSGLSNGERYDEWQKIRDDRARVSVGARSSVFAPFKNLGLIIIDEEHE
                     STYKQEDYPRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKDVYHLLSLPNRVN
                     QQALPEIDIVDMREELSEGNRSMFSKDLREAIQLRLDRQEQVVLFLNRRGYASFMLCR
                     DCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGTGTQKVE
                     ELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITL
                     VGVLNADTMLNLPDFRASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQK
                     NDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKVMEASQHVHKILLQHLTEKAL
                     VLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDP
                     QMMM"
BASE COUNT         6992 a         2870 c         3744 g         6143 t
ORIGIN      
        1 caagttgcta ttagtttact ttttgcaggt gcacttggaa actttattga tagaatttta
       61 acaggagaag ttgttgactt tattgataca aatatttttg gttatgattt tccaatattt
      121 aatatagcag attcaagttt aacaattggt gtaatattaa ttattattgc cttattaaag
      181 gatacttcca ataaaaagga gaaggaggtt aagtaatgga gacttatgaa tttaacatta
      241 cagataaaga acaaacaggt atgcgtgtag ataagttgct gcctgaatta aataatgatt
      301 ggtctcgtaa ccagatacaa gattggatta aagcaggttt agtcgttgca aacgataaag
      361 ttgttaaatc taattataaa gtgaaactta atgatcatat agttgtcact gaaaaagaag
      421 tggttgaagc tgatattcta cctgaaaatt taaatttaga tatttattat gaagatgacg
      481 atgttgcagt tgtatataaa ccgaaaggca tggtagttca tccatcacca gggcattata
      541 ccaatacatt agttaatggt ttaatgtatc aaattaaaaa tttgtcaggt attaatggag
      601 aaattcgtcc aggtattgtt caccgtatag atatggatac ttctggttta ttaatggttg
      661 ctaaaaatga tattgctcat cgtgggcttg tagaacaatt aatggataaa tctgttaaaa
      721 gaaaatatat cgctttagtt cacgggaata ttcctcatga ttacggtaca atcgatgcgc
      781 caattggtag aaacaaaaat gatcgtcaat ctatggctgt tgttgatgat ggtaaggaag
      841 cagtgacaca ttttaacgta ctagaacatt ttaaagatta tacgcttgtt gaatgtcaac
      901 ttgaaacagg acgtacgcat caaatccgtg tgcacatgaa atatattggc ttcccattag
      961 ttggtgatcc aaagtatgga ccgaaaaaga cattggatat tggtggtcaa gctctacatg
     1021 ctggacttat tggattcgaa catccagtaa caggtgaata tattgaaaga catgctgaat
     1081 taccacaaga ctttgaagat ttattagata caattcgaaa aagagatgca taattgtgtt
     1141 gtgctataat tacgataaca ttattatgta aactataagt atttatcgtt ttcgtttttt
     1201 taaaatgaaa acacattata aaatagatta aggttaacat ttttactgag atcggttaat
     1261 ttttcaatat agattgacaa agcgcctgat tagtattatc cttataacag tttaagtaca
     1321 acataaagag tctttaattg agcgtccaga gagacgtcaa agacatgaaa atatagatat
     1381 atactaaaat gatcataaca ctttcacata tgcaagaata agtagtaata ctacgattct
     1441 tctatgtaaa taggctgatt gtatatacta aacctcatgt cgatattaaa gacatgaggt
     1501 tttttgtttc taaattttga aagggtgaga atatgtctga acgtattata atggatgatg
     1561 ccgcaataca acgtacagtg acgagaatcg ctcacgaaat tttggagtat aacaaaggta
     1621 ctgataattt aattctttta ggtatcaaaa caagaggtga atatttagcg aatcgtatac
     1681 aagataaaat tcatcaaatt gagcaacaac gtatacctac tggaacaatt gatattacat
     1741 actttagaga tgatatagag cacatgtcat cacttacgac aaaagacgca atagacatcg
     1801 acacagatat tacagataaa gtagtcatca ttattgatga tgtgctgtat actggtcgaa
     1861 cggttcgtgc ttcacttgat gctattttgc taaatgctag acctattaaa attggtttag
     1921 ctgctttggt tgatcgagga catcgtgagt taccaattcg agcagatttt gttggtaaaa
     1981 atatacctac ttctaaagag gaaacggtaa gtgtctattt agaagaaatg gatcaaagaa
     2041 atgcagttat aattaaataa tacccctttt aattcagtac gagagactga aaaaggtggg
     2101 ggagttgaat gataaataag ttttcatatg cttgtttatc taataatacc ctcatgttcg
     2161 acaactataa gtcaatttct atggacgaaa atgacttgta gcaacttgtc acactttttg
     2221 tctctttaca tatactgatg taaagagatt tttttattat cggaggaaat gaatattatg
     2281 caaaatgatg aaatgtttga acgaacagta aaaccagtac tagatgtaaa tgaaaaacca
     2341 caaccagcgc aatgggcatt tttaagctta caacatttat ttgcgatgtt tggcgcaaca
     2401 gtactagtac cattcttaac aggactacca atatccgcag cgttactagc ttcgggaatc
     2461 ggtacattac tttatatctt aataacgaag gcgcaaatac cagcatactt gggatctagc
     2521 tttgcattta tcacaccaat tatcacggga ttaagtacgc atagcttagg agacatgctt
     2581 gtagcattat tcatgagtgg tgttatgtac gtcatcatcg gaattctaat caaattaagt
     2641 gggacagcat ggttaatgaa attattacca ccagttgttg ttggaccagt catcatggta
     2701 attggtttaa gcttagcgcc tactgcagtc aatatggcaa tgtatgaaaa tcctggagat
     2761 atgaaaggtt acaatatcag tttcttaatt gttgcaatga taaccttgct tgtaacaata
     2821 gtcgttcaag gattttttaa aggattccta tctttaattc cagtacttgt aggtattatc
     2881 gtaggttatg tggtagcgat ttttatgggg attgtgaaat ttgatgcaat tatgtcagca
     2941 aaatggatag atttccctca tatttatctg ccatttaaag attatgtacc ttcatttcac
     3001 ttaggacttg tacttgtaat gattccgatt gtgtttgtaa cagtaagtga acatattggg
     3061 caccaaatgg tattgaataa aatcgtaggt agaaacttct ttgaaaagcc aggacttgat
     3121 aaatcaatca ttggtgatgg tgtttctaca atgtttgcca gtattattgg tggaccacca
     3181 agtacaacat acggtgaaaa tatcggtgta ttagcgatta caagaatata cagtatttac
     3241 gtcattggtg gtgcagcagt tatagcaatt gttttagcat tcattggtaa gttcactgca
     3301 ttaatttctt ctatacctac accagttatg ggaggagtat ctatattact tttcggtatt
     3361 attgcagcaa gtggcttaag aatgttagtt gaaagcaaag tagattttgc gaacaatcga
     3421 aatttagtta tagcttctgt aattttagtt gtaggtatcg gtaatttagt atttaactta
     3481 aaagaaattg gtatcaacct tcaaattgag gggatggcat tagctgcact ttcaggaatt
     3541 attttgaact taatcttacc taaagagaaa aaacaaaaca attaagattt acaaattaag
     3601 gagggcgctt ttatgaatca tttattatca atggaacatt tatctacaga tcaaatatac
     3661 aaacttatcc aaaaggcaag tcaatttaaa tctggtgaac gtcaactacc aaactttgaa
     3721 gggaaatatg tcgcaaattt attctttgaa aattctactc gaacaaaatg tagttttgaa
     3781 atggcagaac ttaagctagg gttaaaaacg attagctttg aaacatcaac atcatctgtt
     3841 tcaaaaggtg aatctttata tgacacatgt aaaactttag aaagtattgg ctgtgattta
     3901 ttagtcatta gacatccgtt taataactac tatgaaaaat tagcgaatat taacatccca
     3961 attgcgaatg ctggtgatgg tagtggacaa catccaacac aaagtttact tgatttaatg
     4021 acgatatatg aagaatatgg atattttgaa ggcttgaatg tattgatttg tggagacatt
     4081 aaaaattcac gtgtcgcacg tagtaattac catagtttaa aagcattagg tgcaaacgta
     4141 atgtttaata gcccaaatgc ttggattgat gattctttag aagcacctta tgtaaatata
     4201 gatgatgtta tagaaacagt agatatagtt atgttattaa gaattcaaca tgaaagacat
     4261 gggcttgcag aagaaactag atttgcagca gatgattatc atcaaaagca tggcttaaat
     4321 gaagtgcgct ataacaaatt acaagaacat gctattgtta tgcatccggc acctgtgaat
     4381 agaggagtag aaatacaaag cgatttagta gaagcttcaa aatcaagaat ttttaagcaa
     4441 atggaaaatg gcgtttactt aagaatggca gtcattgatg aattattaaa ataggtaagg
     4501 ggacgaaaat gatgaaatta attaaaaacg gtaaagtatt acaaaatgga gaattacaac
     4561 aagcagatat tttaattgat ggtaaggtaa ttaaacaaat tgcacctgca attgaaccaa
     4621 gcaatggtgt tgacatcata gatgcgaaag gtcactttgt gtcacctgga tttgtcgatg
     4681 ttcatgttca tttacgtgaa cctggtggtg aatataaaga gacaattgaa actggtacta
     4741 aagctgctgc tagaggcgga tttacaactg tatgtccaat gcctaacaca agaccggtac
     4801 cagattctgt agaacatttt gaagctttac aaaaattaat cgatgacaat gctcaagtac
     4861 gtgtattacc ttatgcttca attacaacac gtcaattagg taaagaattg gttgatttcc
     4921 cagcactagt aaaagaaggt gcctttgcgt ttacagatga cggtgtagga gtacaaactg
     4981 caagcatgat gtatgaaggc atgattgaag ctgcaaaagt aaacaaagcc atcgtagcac
     5041 actgtgaaga taattcatta atctatggtg gtgcaatgca tgaagggaaa cgcagtaaag
     5101 agttaggtat accaggtatt ccaaacattt gtgaatctgt tcaaatcgca agagatgtac
     5161 tattagctga agcagcaggt tgtcattatc atgtatgtca tgtttctact aaagaaagtg
     5221 ttagagtcat tcgtgacgct aaacgcgcag gcattcatgt tacagctgaa gttacaccac
     5281 accatttatt gttaacagaa gatgatattc ctggtaataa tgccatttat aaaatgaatc
     5341 caccattgag aagtactgaa gatagagagg ctttgttaga agggttacta gacggtacaa
     5401 ttgactgtat cgcaacagac catgcaccac atgcacgtga tgaaaaagca caaccaatgg
     5461 aaaaagcacc attcggaatt gttggtagtg aaacagcatt cccattatta tatacgcatt
     5521 ttgtaaaaaa tggtgattgg acattacaac aattagtaga ttacttaaca attaaaccat
     5581 gtgagacatt taatttagaa tacggcacat taaaagaaaa tggttatgca gatttaacaa
     5641 tcattgattt agatagtgaa caagaaatta aaggagaaga tttcttatca aaagcagata
     5701 atacaccatt tatcggctat aaagtttatg gaaatccgat cttaacaatg gttgaaggcg
     5761 aagttaaatt tgagggggat aaataatatg caaagcaaac gttatctagt gttagaagac
     5821 ggttcttttt acgaaggcta ccgtttagga tctgataact taactgtagg agaaattgta
     5881 tttaatacag ctatgacagg ttatcaagaa actatttcag atccatcata tacaggtcag
     5941 atcattactt ttacgtatcc attaatcggt aattatggta tcaatagaga cgattttgaa
     6001 tcattagtac ctacattaaa cggtattgta gtgaaagaag cgagtgcgca tccaagtaat
     6061 tttagacaac aaaagacact tcatgacgtt ttagaattgc atcaaattcc agggattgca
     6121 ggtgttgata caagaagtat tacgcgtaaa attcgacaac acggtgtgtt aaaagctggt
     6181 tttactgatc gaaaagaaga tattgatcaa cttgtcaaac atttacaaca agtagaatta
     6241 cctaaaaacg aagtagaaat cgtttcgact aaaacaccgt atgtttcgac aggtaaggat
     6301 ctaagtgtcg tacttgtaga ctttggtaag aagcaaaata ttgttcgaga attaaacgtc
     6361 agaggttgta acgtcacagt tgtaccatat acaactactg ccgaagaaat tttagcaatg
     6421 gctccagatg gcgttatgct atcaaacgga ccaggtaatc ctgaagttgt agaatgtgcg
     6481 attccaatga ttcaaggaat tttagggaaa attccgttct ttggtatctg tctaggacat
     6541 caactttttg cattatctca aggagcaagc tcatttaaaa tgaagtttgg tcatcgtggt
     6601 gcgaaccatc cagttaaaaa tttagagact ggaaaagttg atattacgag tcaaaaccat
     6661 ggatatgcaa tagatataga ttcgttaaaa agtactgatt tagaagttac tcatcttgca
     6721 ttaaatgatg gtactgtaga aggtttaaaa cataaaacat taccagcatt ttctgttcaa
     6781 taccatcctg aagcaaatcc aggaccgtca gattcaaact atctatttga tgattttgta
     6841 gcaatgatga ctaattttaa ggaaaaggag cgtcatatca atgcctaaac gtaatgatat
     6901 caaaacaatt ttagtaatag ggtctgggcc aattatcata ggtcaagcag ctgaatttga
     6961 ttatgctgga acacaagcat gtctagcttt aaaagaagag ggatatcgag ttattcttgt
     7021 aaattcaaat ccagcgacaa tcatgactga taaggaaatt gcggataaag tatatatcga
     7081 accgttaact catgatttta tagcgcgaat tatacgtaaa gagcaacctg acgctttact
     7141 tccaacttta ggtggtcaaa caggtttaaa catggcgatt caactacacg aaagtggtgt
     7201 gcttcaagat aataacgtcc aattattagg aactgagcta acatcaattc aacaagcaga
     7261 agaccgtgaa atgtttagaa cattaatgaa tgatttaaac gttcctgtac cagagagtga
     7321 cattgtaaat acagtagagc aagcctttaa attcaaagag caagtgggat acccgctaat
     7381 tgttagaccg gcatttacga tgggtggtac cggaggcggt atttgtcata atgatgaaga
     7441 attacatgaa atcgtctcaa atggtcttca ttatagtcca gcaacgcaat gtttattaga
     7501 aaaatctatc gcaggtttta aagaaatcga atacgaagta atgcgtgata aaaacgataa
     7561 tgccatcgtt gtatgtaaca tggaaaatat tgatccagtt ggtattcata ctggcgattc
     7621 aattgttgtg gctcctagtc aaacattatc agatgttgag tatcaaatgt tacgtgatgt
     7681 ttcattaaaa gttattcgag ctttaggtat cgaaggtggt tgtaatgttc aattagcatt
     7741 agatccccat tcattcgatt attatattat agaagtaaat ccgcgtgtat cacgttcatc
     7801 agcgttagct tcaaaagcaa caggatatcc tattgcaaaa ttagctgcta aaatcgcggt
     7861 tggtctaaca ttagatgaaa tgttaaatcc aattacagga acatcttatg cagcgtttga
     7921 accaacttta gactatgtga tttcaaaaat accaagattt ccttttgata aatttgaaaa
     7981 aggagaacga gagcttggca cacaaatgaa agcaacaggt gaagttatgg ccattggtcg
     8041 aacttacgaa gaatcattgt taaaagcaat tcgatcactt gagtatggtg tgcatcactt
     8101 aggattacca aatggtgaaa gcttcgatct tgattatatt aaagaacgta tttcacacca
     8161 agatgatgaa cgattatttt tcatcggcga agcaattaga agagggacaa cattagaaga
     8221 aattcataat atgactcaga ttgattactt cttcttacac aagttccaaa acattattga
     8281 tattgagcat caattaaaag agcatcaagg tgatttagaa tatcttaaat atgcaaaaga
     8341 ttatggattt agtgataaaa caatagcgca tcgctttaat atgacggaag aagaagtata
     8401 tcaattgcgt atggaaaatg atattaaacc tgtttacaag atggttgata cttgcgcagc
     8461 tgaatttgaa tcttcaacac catattatta tggtacatac gaaactgaaa atgaatccat
     8521 agttactgac aaagaaaaaa tcttagtatt aggctctgga ccaattcgaa tcggccaagg
     8581 tgtagaattt gactatgcga cagttcacgc cgtttgggca attcaaaaag cagggtacga
     8641 agcgataatt gtgaataaca atccagaaac agtttcaaca gacttctcaa tttctgacaa
     8701 attatacttt gaacctttaa ctgaagaaga tgtgatgaat atcattaatt tagaaaaacc
     8761 taaaggtgtc gttgtacaat ttggaggaca aacagcgatt aatttagcag acaaattggc
     8821 taaacatggt gttaaaatac ttggtacttc actagaaaat ctaaatcgtg ctgaagatag
     8881 aaaagaattt gaagcactat taagaaaaat taacgtgcca cagccacaag ggaaatcagc
     8941 tacatcacct gaggaagcat tagcgaatgc tgcagaaatc ggatatccgg ttgtagtaag
     9001 accttcttat gtattaggtg gtcgcgcaat ggaaattgta gacaatgaca aagagttaga
     9061 aaactatatg acccaggctg taaaagcgag tccggaacat ccggtactag tcgatagata
     9121 tttaactggt aaagaaattg aagttgatgc gatttgtgat ggagaaacgg tcattattcc
     9181 aggaatcatg gaacatattg aacgtgctgg tgtacatagt ggtgactcaa tcgctgtata
     9241 tccgccacaa actttgacag aagacgagtt agcaacactt gaggactata ctataaaatt
     9301 agctaaaggt ttaaacatca ttggcttaat caacattcaa ttcgttatag ctcacgatgg
     9361 tgtatatgtt ttagaagtaa acccacgttc tagtagaacg gtaccattct taagtaaaat
     9421 tactgatatt ccaatggcac aattagctat gcgagcaatc attggggaaa aactaacaga
     9481 tatgggttat caagaagggg ttcaaccata tgctgagggt gtatttgtga aagcaccggt
     9541 atttagtttt aataaattga aaaatgttga tattacttta ggacctgaaa tgaagtctac
     9601 aggtgaagta atggggaaag atactacatt agaaaaggcg ttattcaaag ggttaacagg
     9661 tagtggtgtt gaagttaaag atcacggtac agtattaatg accgtcagtg acaaagataa
     9721 agaggaagtt gttaaattgg cacaacgctt aaatgaagtt ggctataaaa ttttagcaac
     9781 gtctggaaca gctaataaat tagctgagta tgacatacct gcagaagtag taggcaaaat
     9841 cggtggcgaa aatgatttat taacacgtat tcaaaatggt gatgttcaaa tcgttataaa
     9901 tacaatgact aaaggtaaag aagtagaaag ggatggcttc caaattagac gtactacagt
     9961 tgaaaatggt attccatgtt tgacatcttt agatacagct aatgccttaa cgaatgtaat
    10021 tgaaagtatg acatttacaa tgcgtcaaat gtaaatcaat caaactgtat cggtggggct
    10081 gtaattaacc atttacttaa agaagtttat attacagcct cattatttta atgaatttct
    10141 taatataaag ggagacatat atgatgaaag atttaccaat tattgcatta gattttgaat
    10201 caaaagaaaa agtaaatcaa tttttagatt tatttgatga atcattattc gtaaaagtag
    10261 gtatggaact tttttatcaa gaaggtcctc aattaattaa tgagataaaa gaaagaggcc
    10321 atgatgtatt tttagattta aaactgcatg atattcctaa tacagttggt aaggcgatgg
    10381 aaggactagc taaattgaat gttgatctgg taaatgttca tgctgctggt ggcgtaaaaa
    10441 tgatgtctga ggccattaaa ggattaagaa aacataatca acatacaaaa attattgcag
    10501 taacacagct tacgtcaaca acagaagaca tgttacgaca cgaacaaaac atacaaacat
    10561 cgattgaaga ggccgtttta aattatgcca agttagcaaa tgcagctggt ttagatggcg
    10621 ttgtttgttc acctcttgaa agtcgtatgt tgactgaaaa gttaggtaca tcatttttaa
    10681 aagtaacacc aggtattaga cctaaaggtg catctcaaga tgaccaacac cgtattacga
    10741 caccggaaga agcaagacag cttggttcga cgcatattgt agtcggtaga ccgattacac
    10801 aaagtgacaa tccagtcgaa agttatcata aaattaaaga aagttggtta gtataatggc
    10861 taaagaaatt gcaaaatcat tattagatat tgaagctgta acattatcac caaatgattt
    10921 atatacatgg agttcaggta ttaaatcacc gatttactgt gataaccgtg ttacgttagg
    10981 ttatccttta gttcgaggcg caatccgcga tggtttaatt aacttaatta aagaacactt
    11041 tcctgaagta gaagttattt ctggtactgc aacagctggt attccacatg cagcttttat
    11101 tgctgaaaaa ttaaaattac caatgaatta tgttcgttca tcaaataaga gtcatggtaa
    11161 gcaaaatcaa atcgaaggtg ctaaaagtga aggtaaaaaa gtagttgtga tagaagattt
    11221 aatttcgaca gggggatctt cagtcacagc agttgaagcc ttaaaacaag caggtgcaga
    11281 agtattaggt gttgtagcta tctttactta cggtttgaaa aaagcagatg atacatttag
    11341 caatattcaa ctaccttttt acactttaag tgattacaat gaattaattg aagtagctga
    11401 aaatgaaggt aaaatttcta gtgaagatat ccaaacatta gttgaatgga gagacaactt
    11461 agcataatat agacactaga aggaggaatt caacaaatga atgacaaaac atctaatgat
    11521 ttatatggga agataaaaca ttgtaacgaa tttatcaatc attcaaatga ttccaatcta
    11581 tctagtagtc acgatgtcga cgaaagttca acgaagcaaa aacatataaa aaataaaaca
    11641 actatagatc ataatgatga tttatttaaa catgtaaagg atatattacg taaacaagga
    11701 caaatttaaa aacaaaaaag ctatagtgtt taatatcagg ggctgcttaa ccgcatccga
    11761 atattacaaa cactatagct tttattatat aaaaaattta ttagcggata attaacttta
    11821 ttaaaatcca accaattaag aatacgagta ataggactaa tactggaatc acataatgta
    11881 acataacgtc cctcctttaa cttaatttta attgtaatca aatttgacaa taagtcaaaa
    11941 cattaatacc tatgataagt atcatttatt aacatatgta tcatattttt aatcttgcgt
    12001 aatttttatc gttaactatg tgatttaatc aacaggccaa taagacgttt gtcattcgtg
    12061 cacataacgg tatgacgcgc ttttgtcatt ttaattatgt taagataata gtagttatag
    12121 aagttcgatc tataggaggc atagcatgga tattccaaaa atcacgacat ttttaatgtt
    12181 taataacaaa gctgaagaag ctgttaaact atacacaagc ttatttgaag atagtgagat
    12241 tataacaatg gctaagtatg gtgaaaatgg acctggtgat cacgggactg tacaacactc
    12301 aatatttaca ttaaatggac aagtattcat ggcgattgat gctaatagtg gcacagaatt
    12361 accaatgaat cctgcgattt cattatttgt tacagtaaaa gatactattg aaatggaacg
    12421 actatttaat ggattaaaag atgaaggtgc cattttaatg ccaaaaacga atatgccacc
    12481 atacagagag tttgcttggg ttcaagataa gtttggagta agttttcaat tagcattacc
    12541 tgagtaaaag gattcgcaca gcgttgaatg ataaagaaac actttttctt atgcatgcgt
    12601 tttacctatg tatttctatt ttaagtacac attagcattt tagtttcact catttttaaa
    12661 atcacaaaga taatttgtaa cttaaatgat gatacgttat ttaaaaacac gatacttcgt
    12721 ttcaatgaac gcattaaata ataaataaac acctcgcaaa agaaagtagt cctttcttat
    12781 ggtgaggtgt ttatttgtcg acaacttgat tataattgtt tcattttttg aataagttca
    12841 taatcaggtg tagcatacaa agttttttga ttgtcatatg ttacaaaccc aggctttgca
    12901 cctgatggtt tgtgcacatt tttaattaat gtgtaatcaa caggtatttg tccagaatta
    12961 ccagcttttg aaaagtatcc tgctaacata gccgcttcct tgattgtcgt atcacttggt
    13021 gcatcattaa atataacgac atgtgaacca ggaatatctt ttgtgtgtaa ccatgtgtga
    13081 gtttttttag cttttttatt tgttaaataa tcattttgct tgttattctt gccaacatat
    13141 atatcgtcgc catcagttga tacataatgt tgtaattgaa tctgcgcttt ctttttctta
    13201 gtttgatttt tacgctgttt cataaagcct tgttctgcta attcatctct aatttcatca
    13261 atgtcatgga cagaaatatg atgtaattgt tgttcgattg ttgaaaaata atctatatta
    13321 tctttcgtca attgaatttg atgttgtaat tcacgttctc tcgttttcat acgattatat
    13381 tgtttataat aatattgagc atttgctgat ggggattttg taggatttaa aggaatgaca
    13441 acttcttcat tcgtataata attcaatgct gtcacttctt tatcgccttg cttaattcga
    13501 tatatattag cagtgatcaa ttcaccatat aactgttcgg tatctttatt tttagactgc
    13561 tcatattctt caatcaactt cgctaattta ttttgatatt tgtgcaactg ctgttgaaca
    13621 aatcgaacta aatcattcgc acgttgttta acgcgttcac gttcgccacg cgcgtcataa
    13681 aaacgatcaa gtaaatcatt taatgaatcg tacgtaactg tatcatcatt aaattgattt
    13741 aactttataa aatagaaatc ctctttacct gtttcatgat ttttatgaaa aataggagta
    13801 ggtggtaact tggtttctgc cattacttcg tcgaatgctt ctggtaatgt tgatgaagtc
    13861 ataaattgac gacgactaac gatttcattc gtaattaaag ggctaaatcc ttcaaactga
    13921 ttcaataatt gtttagcaat attacctgcg ttaaaatcga tatatttcaa cacctctgca
    13981 cctgtaatat catacggatt tattttgtgc tgagtaggtg gtgcttcata attaaatcct
    14041 ggcattactg tacgatagtg attcgtattt ggtgttaagt gtttaaatcc ttcaattatt
    14101 ttgcgatttt catctactaa aattaagtta ctatgtttac ccataatctc aaggatgaca
    14161 gtgcggtaaa tagtatcgcc aatttcatct ttactcttta tatcgatttc aatgcgacga
    14221 tcattaccaa tttgcttaat cgattcgata ataccacctt ctaagtgttt tctaaaaaca
    14281 cgcgcaaaca tgggtggatt aaatggatta tcatattttt tagtagtcaa ttgtaatctt
    14341 gaaaagtttg gatggattga caataacaat tgatggtttt gtctattttg acgtacaacc
    14401 attagtatcg tgtcattatc aggttgattg attttgtgaa cgcgtcctgt tgttaaaaat
    14461 tgtagagact caaccatttt ctttgtaaat aatccatcat aagccataat ttatcttcca
    14521 ccttcttcat atcattcgtt tcattttaac atgattgtaa cttttcggta tagttattga
    14581 accatacatt atgccacaca ttttacatta tctaaaagtt cgcaattcaa tttatttaat
    14641 taaaattaat cgtgcaatat aacgatattg ttagacttaa tagaaattat ggcatgcaat
    14701 ttcaaatatg ctatacaata taaagaacaa tgtgatatca tatttaaata atagaagatt
    14761 agcttagaga ggtcgtaagg catggataat gaaaaaggat tgttaatcgt tttatcagga
    14821 ccatctggag taggtaaagg tactgttaga aaacgaatat ttgaagatcc aagtacatca
    14881 tataagtatt ctatttcaat gacaacacgt caaatgcgtg aaggtgaagt tgatggcgta
    14941 gattactttt ttaaaactag ggatgcgttt gaagctttaa ttaaagatga ccaatttata
    15001 gaatatgctg aatatgtagg caactattat ggtacaccag ttcaatatgt taaagataca
    15061 atggacgaag gtcatgatgt atttttagaa attgaagtag aaggtgcaaa gcaagttaga
    15121 aagaaatttc cagatgcgtt atttattttc ttagcacctc caagtttaga tcacttgaga
    15181 gagcgattag taggtagagg aacagaatcc aatgagaaaa tacaaagtcg tattaacgaa
    15241 gcgcgtaaag aagttgaaat gatgaattta tacgattacg ttgtagttaa tgatgaagta
    15301 gaacttgcga agaatagaat tcaatgtatt gtagaagctg agcacttaaa aagagagcgc
    15361 gtagaagcta agtatagaaa aatgattttg gaggctaaaa aataatgtta aatccacctt
    15421 taaaccaatt aacgtcacaa attaaatcaa agtatttaat tgcaacaact gcagcgaaaa
    15481 gagcgcgtga aattgatgaa caacctgaaa ctgaattatt aagtgaatat cattcattta
    15541 aaccagttgg tagagcgtta gaagaaattg ctgacggtaa aattcgccct gttatttcaa
    15601 gtgattatta tggtaaagaa tagtttcgac cattaaaaat atgtgaagct agacgtacat
    15661 caaatacgtc aaggcatatc caaatcaaac gcacctatgt aggtgtgttt ttttagttta
    15721 tccaatgatt gatgttataa taatactaaa tttgtatcta taaaaaagta atgagcattt
    15781 gtgcgcatat gatgatgtaa agcgtaaatg tcatagtgat atttaaggga gattgaatat
    15841 gaagaaaata ttattagccg ttacaggtgg cattgcggca tataaagcaa ttgatttgac
    15901 aagtaagtta acacaatctg ggtatgaagt tcgcgttatg ttaacgaatc acgcacaaaa
    15961 atttgtgaca ccattagcat ttcaagcaat aagtcgaaat gctgtttata cagatacttt
    16021 tatagaagaa aatccttcag aaatacagca tattgcatta ggtgattggg cagatgcaat
    16081 cattgttgca cctgcaacgg caaatacaat tgcaaaattg agtgtaggta ttgctgatga
    16141 tttggtgaca tcaacgttgc tagcaacaga gacaccgaaa tttattgcgc ctgctatgaa
    16201 tgtgcatatg tatgaaaata aacgtacgca gcaaaatatt aatattttaa aagaagatgg
    16261 gtatcatttt atcgaaccag gaagcggatt tctagcatgt ggttatgttg ctaaaggacg
    16321 tatggaagaa ccgcttcaaa tcgtttctgt tattgatgct cattttcaaa atagtaatcg
    16381 tttagctaat agttcatttc aagataaacg cgcattggtt acagcaggac caactattga
    16441 agttatcgat ccagtcagat ttgtatccaa tcgttcttct ggaaaaatgg gctatgcaat
    16501 agctgaagca ttgcgaaatc gaggagctat cgtgacgtta gttgctggtc ctacaacact
    16561 agaggatcca aaagatattg aagttattca tgttcaaagt gctgaagaaa tgtttgaaca
    16621 agtgacaagc cgatttgacg aacaagatat tgttgtaaaa gcagcagccg tatctgacta
    16681 tacaccggtt gatgtattag aacataagat gaaaaagcag gatggtgatt tgtcagtatc
    16741 ttttaaacgt actaaagaca ttcttaaata tttaggtgaa cataaaacat cacagtattt
    16801 aataggcttt gcagcagaga ctgaagatat tgaaaattat gcacaacaaa aattacgcaa
    16861 gaaaaatgca gatgtgatta tttcaaataa tgttggggat atgtctatcg gatttagttc
    16921 tgatgataat gaattgacaa tgcattttaa aaataatgaa aaggtaaata tcaagaaagg
    16981 aaaaaaagta gtattagctg cacaaatttt agatgaacta gaaactaggt ggcaataatg
    17041 atagcgaaag tcatagtcga tgtcgcgtcg aagagcgttg actataaatt tgattatata
    17101 attcccgaac aactcgaatc tgtcatccaa cctggtgtgc gtgtaattgt accttttgga
    17161 ccaagaacga ttcaaggtta tgtaatggaa gtaacagcag aacctgatgc acaacttgac
    17221 gtttcgaagt taaaaaaaat catagaagtg aaagatatac aaccagaatt aacatcagaa
    17281 ttaatagctt taagtgagtg gatgggttca actcatgtca ttaaacgtat ttctatgcta
    17341 gaagtgatgc ttccaagtgc tattaaagcg aagtataaaa aagcatttaa gatgaaagat
    17401 gacatagagc taccttcagc tttattacaa aaatttgata agcatggtta ctattattat
    17461 aaagatgcgc aaaaaaataa tgatattcaa ttgcttatga agttgttaaa agatgatatc
    17521 gttgaagaaa aaacgattct cacacaaaat ataactaaaa aaaccaagcg tgctgttcgt
    17581 gtcattgaag ggtatcatcc tgatgaagta ttagctaagt tggagaaagt tattaaacaa
    17641 tacgatttgt atgcttactt gtctgaagaa caacataaaa caatattttt aactgatatt
    17701 gaggatatgg gcttttcaaa atccagttta gatggactta tcaaaaaagg ttatgttgaa
    17761 aaatatgacg cggttgttga aagagaccca tttaaagatc gtgttttcga acaagaatca
    17821 aaacagcaat taacagaaga ccaatataaa gcatatgaag cgattaaagc taaaattgta
    17881 agccaagagc aagaaacatt tttacttcat ggtgtgacgg gatcaggtaa aacagaagta
    17941 tatttacaaa cgatagaaga tgttttaagc caaggaaaac aggcgatgat gttagttcct
    18001 gaaatcgctc taacaccgca aatggtttta cgcttcaaac gtcgatttgg tgatgacgtt
    18061 gctgtattac attctggctt atctaatggg gaacgttatg atgagtggca aaaaattagg
    18121 gatgatcgtg cgagagtaag tgttggtgca aggtcaagtg tgttcgcacc tttcaaaaat
    18181 ttagggttaa tcatcattga tgaagaacat gaatctacat ataaacaaga agattatccg
    18241 agatatcacg ctagagaaat tgcccaatgg cgaagtgaat atcatcactg tccagtcatt
    18301 ttaggaagtg caacaccatg tcttgaaagt tatgcacgag ctgaaaaaga cgtttatcat
    18361 ttgctatcat taccaaacag agtgaaccaa caagctttac ctgaaattga tatagtagac
    18421 atgcgtgaag aattgagtga aggtaatcgg tcaatgtttt caaaagattt acgtgaagcc
    18481 atacaattaa gattagatcg acaggaacaa gttgttttat ttttaaatcg acgtggttat
    18541 gcatcgttta tgttatgtcg ggattgtgga tatgtaccgc aatgtccaaa ctgtgatatt
    18601 tcattaacgt atcataaaac gacagactta ttaaaatgtc actattgtgg ttaccaagag
    18661 acgccaccga atcaatgtcc aaattgtgag agtgaacaca ttcgacaagt aggtactggt
    18721 actcagaaag ttgaagaact attgcaacaa gaatttgaag atgcgcgcat aattaggatg
    18781 gatgtagata caacctcaaa gaaaggtgca catgaaaagt tattgactga attcgaaaaa
    18841 ggtaacggtg acattttact aggtactcag atgattgcga aaggattaga ttatccaaat
    18901 attactttag ttggtgtgct gaatgcagat acaatgttaa atttacctga ttttcgggcg
    18961 agcgaacgta cttatcaact attaacgcaa gtggctggta gagctggtcg tcatgaaaag
    19021 gcaggtcaag tcatcattca aacgtataat ccagatcatt attcaatatt ggatgttcaa
    19081 aaaaatgatt atttaacatt ttatcgtcag gaaatggaat atcgtaaatt aggaaagtat
    19141 ccaccgtatt attatttgat taatttcaca atctcacata aagaaatgaa gaaggttatg
    19201 gaagcatcgc agcatgttca taaaatttta ttacagcatt taacagaaaa agcgcttgta
    19261 ctaggtccat ctccggcagc acttgcgaga atcaacaatg aatttagatt ccaaatttta
    19321 gtgaaatata aaagtgaacc tggattatta caagccattc agtttttaga tgactattac
    19381 catgaaaaat ttataaaaga aaaattagca ttgaagattg atattgatcc acagatgatg
    19441 atgtaacatt actaattatt agaaacaagt tcagtatgta cgagtatttg aaccagtgtt
    19501 tgaatattta ctttaagtac agaaaaaggg cagaatatac aactgttaac tatttaaatt
    19561 agcagtttat attctgtctt tttatatggc tttataactt acgtgatttt ggtttgataa
    19621 ggaatttatt agtattttca tttacgaatt ctgattttaa tgcaagcgcc atacttactg
    19681 atgaataaaa gtactaatga ttaaacaaac tatttttaaa tggtaaattg aaatagtgca
    19741 aattttaca
//
LOCUS       DANBWH010000025        19096 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.025, whole genome shotgun sequence.
ACCESSION   DANBWH010000025 DANBWH010000000
VERSION     DANBWH010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19096
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.025"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            154..1344
                     /locus_tag="PPY92_000614"
     CDS             154..1344
                     /locus_tag="PPY92_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HDJ2624637.1"
                     /translation="MTKSEKIIELTNHYGAHNYLPLPIVISEAEGVWVKDPEGNKYMD
                     MLSAYSAVNQGHRHPKIIQALKDQADKVTLVSRAFHSDNLGEWYEKICKLAGKDKALP
                     MNTGAEAVETALKAARRWAYDVKGIEPNKAEIIAFNGNFHGRTMAPVSLSSEAEYQRG
                     YGPLLDGFRKVDFGDVDALKAAINENTAAVLVEPIQGEAGINIPPEGYLKAIRELCDE
                     HNVLFIADEIQAGLGRSGKLFATDWDNVKPDVYILGKALGGGVFPISVVLADKEVLDV
                     FTPGSHGSTFGGNPLACAASIAALDVIVDEDLPGRSLELGDYFKEQLKQIDHPSIKEV
                     RGRGLFIGVELNESARPYCEALKEEGLLCKETHDTVIRFAPPLIITKEELDLALEKIR
                     HVFQ"
     gene            1453..2697
                     /locus_tag="PPY92_000615"
     CDS             1453..2697
                     /locus_tag="PPY92_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HDJ2624638.1"
                     /translation="MTENNNLVTSTQGIIKEALHKLGFDEGMYDLIKEPLRMLQVRIP
                     VRMDDGTVKTFTGYRAQHNDAVGPTKGGVRFHPDVDEEEVKALSMWMTLKCGIVNLPY
                     GGGKGGIVCDPRQMSIHEVERLSRGYVRAISQFVGPNKDIPAPDVFTNSQIMAWMMDE
                     YSALDKFNSPGFITGKPIVLGGSHGRDRSTALGVVIAIEQAAKRRNMQIEGAKVVIQG
                     FGNAGSFLAKFLYDLGAKIVGISDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEETI
                     SNKELFELDCDILVPAAISNQITEDNAHDIKASIVVEAANGPTTPEATRILTERGILL
                     VPDVLASAGGVTVSYFEWVQNNQGYYWSEEEVNEKLREKLEAAFDTIYELSQNRKIDM
                     RLAAYIIGIKRTAEAARYRGWA"
     gene            complement(3034..3963)
                     /locus_tag="PPY92_000616"
     CDS             complement(3034..3963)
                     /locus_tag="PPY92_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDJ2624639.1"
                     /translation="MTNSSKSFTKFMAASAVFTMGFLSVPTAGAEQTNQIANKPQAIQ
                     WHTNLTNERFTTIAHRGASGYAPEHTFQAYDKSHNELKASYIEIDLQRTKDGHLVAMH
                     DETVNRTTNGHGKVEDYTLDELKQLDAGSWFNKKYPKYARASYKNAKVPTLDEILERY
                     GPNANYYIETKSPDVYPGMEEQLLASLKKHHLLNNNKLKNGHVMIQSFSDESLKKIHR
                     QNKHVPLVKLVDKGELQQFNDQRLKEIRSYAIGLGPDYTDLTEQNTHHLKDLGFIVHP
                     YTVNEKADMLRLNKYGVDGVFTNFADKYKEVIK"
     gene            complement(4205..5584)
                     /gene="argH"
                     /locus_tag="PPY92_000617"
     CDS             complement(4205..5584)
                     /gene="argH"
                     /locus_tag="PPY92_000617"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDJ2624640.1"
                     /translation="MSNKAWGGRFEVQPEEWVDDFNASITFDQTLIDQDIEGSIAHAT
                     MLANQGIISQQDSEQIIQGLKSIQHDYHQDQIQFSASLEDIHLNIEHELIKRIGDAGG
                     KLHTGRSRNDQVATDMHLYTKKQVQDIIALIKSLQSVIVDIASNNVDTIMPGYTHLQR
                     AQPISFAHHIMTYFWMLQRDQQRFEDSLKRIDINPLGAAALSGTTYPIDRHETTALLN
                     FGSLYENSLDAVSDRDYIIETLHNISLTMVHLSRFAEEIIFWSTDEAKFITLSDAFST
                     GSSIMPQKKNPDMAELIRGKVGRTTGHLMSMLMTLKGLPLAYNKDMQEDKEGLFDAVH
                     TIKGSLRIFEGMIQTMTINKERLNQTVKEDFSNATELADYLVTKNIPFRTAHEIVGKI
                     VLECIQQGHYLLDVPLATYQQHHSSIDADIYDYLQPENCLKRRQSYGSTGQSSVKQQL
                     DVAKQLLSQ"
     gene            complement(5574..6779)
                     /locus_tag="PPY92_000618"
     CDS             complement(5574..6779)
                     /locus_tag="PPY92_000618"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDJ2624641.1"
                     /translation="MKEKIVLAYSGGLDTSVAVQWLIDKGYDVVACCLDVGEGKDLDI
                     VYKKALDMGAVECHIIDATKEFSDEYVSYAIKGNLMYENAYPLVSALSRPLIAKKLVE
                     IAEKTNSVGIAHGCTGKGNDQVRFEVAIKALNPSLKAFAPVREWAWSREEEIDYAIKH
                     NIPVSINHDSPYSIDQNLWGRANECGILEDPYAAPPEDAFDLTNALEETPDTADEIIL
                     TFDKGIPVQIDGKTYELDDLILTLNALAGKHGIGRIDHVENRLVGIKSREIYEAPAAE
                     VILKAHKALETITLTKDVAHFKPIIEKQFAEQLYNGLWFSPLTDSLKLFIDSTQQYVS
                     GDVRIKLFKGNAIVNGRKSPYTLYDEKLATYTKEDAFNQDAAVGFIDIYGLPTQVNAM
                     LHGGYSNEQ"
     gene            7130..8461
                     /locus_tag="PPY92_000619"
     CDS             7130..8461
                     /locus_tag="PPY92_000619"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDJ2624642.1"
                     /translation="MTHIQLDFSKTLEFFGEHELKQQQEIVKSIHKTIHEGTGAGSDF
                     LGWVDLPVDYDKEEFSRIVEASKRIKENSDVLVVIGIGGSYLGARAAIEMLTSSFRNS
                     NEYPEIVFVGNHLSSTYTKELVDYLADKDFSVNVISKSGTTTEPAVAFRLFKQLVEER
                     YGKEEAQKRIFATTDKEKGALKQLATNEGYETFIVPDDVGGRYSVLTAVGLLPIATAG
                     INIEAMMIGAAKAREELSSDKLEDNIAYQYATIRNILYAKGYTTEMLINYEPSMQYFN
                     EWWKQLFGESEGKDFKGIYPSSANYTTDLHSLGQYVQEGRRFLFETVVKVNHPKYDIT
                     IEKDSDDLDGLNYLAGKTIDEVNTKAFEGTLLAHTDGGVPNMVVNIPQLDEETFGYVV
                     YFFELACAMSGYQLGVNPFNQPGVEAYKQNMFALLGKPGFEDLKKELEERL"
     gene            8786..9361
                     /locus_tag="PPY92_000620"
     CDS             8786..9361
                     /locus_tag="PPY92_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDJ2624643.1"
                     /translation="MSFHQVEEWFEIFRQFGYLPGFILLYIRAIIPVFPLALYILINI
                     QAYGPILGILISWLGLISGTFTVYLICKRLVNTERMQRIKQRTAVQRLISFIDRQGLI
                     PLFILLCFPFTPNTLINFVASLSHIRPKYYFIVLASSKLVSTIILGYLGKEITTILTH
                     PLRGILMLVVLVVFWIVGKKLEQHFMGSKKE"
     gene            9366..9890
                     /gene="lepB"
                     /locus_tag="PPY92_000621"
     CDS             9366..9890
                     /gene="lepB"
                     /locus_tag="PPY92_000621"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDJ2624644.1"
                     /translation="MKKVVKYLISLILAIIIVLFVQTFVIVGHVIPNNDMSPTLNKGD
                     RVIVNKIKVTFNQLNNGDIITYRRGNEIYTSRIIAKPGQSMAFRQGQLYRDDRPVDAS
                     YAKNRKIKDFSLRNFKELDGDIIPPNNFVVLNDHDNNQHDSRQFGLIDKKDIIGNISL
                     RYYPFSKWTIQFKS"
     gene            9906..10481
                     /gene="lepB"
                     /locus_tag="PPY92_000622"
     CDS             9906..10481
                     /gene="lepB"
                     /locus_tag="PPY92_000622"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDJ2624645.1"
                     /translation="MKKELLEWIISIAVAFVILFIVGKFIVTPYTIKGESMDPTLKDG
                     ERVAVNIIGYKTGGLEKGNVVVFHANKNDDYVKRVIGVPGDKVEYKNDTLYVNGKKQD
                     EPYLNYNLKHKQGDYITGTFQVKDLPNANPKSNVIPKGKYLVLGDNREVSKDSRAFGL
                     IDEDQIVGKVSFRFWPFSEFKHNFNPENTKN"
     gene            10641..14114
                     /gene="addB"
                     /locus_tag="PPY92_000623"
     CDS             10641..14114
                     /gene="addB"
                     /locus_tag="PPY92_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HDJ2624646.1"
                     /translation="MTLHAYLGRAGTGKSTKMLTEIKQKMKADPLGDPIILIAPTQST
                     FQLEQAFVNDPELNGSLRTEVLHFERLSHRIFQEVGSYSEQKLSKAATEMMIYNIVQE
                     QQKYLKLYQSQAKYYGFSEKLTEQIQDFKKYAVTPEHLEHFIADKNMQTRTKNKLEDI
                     ALIYREFEQRIQNEFITGEDSLQYFIDCMPKSEWLKRADIYIDGFHNFSTIEYLIIKG
                     LIKYAKSVTIILTTDGNHDQFSLFRKPSEVLRHIEEIANELNISIERQYFNQLYRFNN
                     QDLKHLEQEFDVLQINRVACQGHINILESATMREEINEIARRIIVDIRDKQLRYQDIA
                     ILYRDESYAYLFDSILPLYNIPYNIDTKRSMTHHPVMEMIRSLIEVIQSNWQVNPMLR
                     LLKTDVLTASYLKSAYLVDLLENFVLERGIYGKRWLDDELFNVEHFSKMGRKAHKLTE
                     DERNTFEQVVKLKKDVIDKILHFEKQMSQAETVKDFATAFYESMEYFELPNQLMTERD
                     ELDLNGNHEKAEEIDQIWNGLIQILDDLVLVFGDEPMSMERFLEVFDIGLEQLEFVMI
                     PQTLDQVSIGTMDLAKVDNKQHVYLVGMNDGTMPQPVTASSLITDEEKKYFEQQANVE
                     LSPTSDILQMDEAFVCYVAMTRAKGDVTFSYSLMGSSGDDKEISPFLNQIQSLFNQLE
                     ITNIPQYHEVNPLSLMQHAKQTKITLFEALRAWLDDEIVADSWLDAYQVIRDSDHLNQ
                     GLDYLMSALTFDNETVKLGETLSKDLYGKEINASVSRFEGYQQCPFKHYASHGLKLNE
                     RTKYELQNFDLGDIFHSVLKYISERINGDFKQLDLKKIRQLTNEALEEILPKVQFNLL
                     NSSAYYRYLSRRIGAIVETTLSALKYQGTYSKFMPKHFETSFRRKPRTNDELIAQTLT
                     TTQGIPINIRGQIDRIDTYTKNDTSFVNIIDYKSSEGSATLDLTKVYYGMQMQMMTYM
                     DIVLQNKQRLGLTDIVKPGGLLYFHVHEPRIKFKSWSDIDEDKLEQDLIKKFKLSGLV
                     NADQTVIDALDIRLEPKFTSDIVPVGLNKDGSLSKRGSQVADEATIYKFIQHNKENFI
                     ETASNIMDGHTEVAPLKYKQKLPCAFCSYQSVCHVDGMIDSKRYRTVDETINPIEAIQ
                     NININDEFGGE"
     gene            14118..17771
                     /gene="addA"
                     /locus_tag="PPY92_000624"
     CDS             14118..17771
                     /gene="addA"
                     /locus_tag="PPY92_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDJ2624647.1"
                     /translation="MTIPEKPQGVIWTDAQWQSIYATGQDVLVAAAAGSGKTAVLVER
                     IIQKILRDGIDVDRLLVVTFTNLSAREMKHRVDQRIQEASIADPANAHLKNQRIKIHQ
                     AQISTLHSFCLKLIQQHYDVLNIDPNFRTSSEAENILLLEQTIDEVIEQHYDILDPAF
                     IELTEQLSSDRSDDQFRMIIKQLYFFSVANPNPTNWLDQLVTPYEEEAQQAQLIQLLT
                     DLSKVFITAAYDALNKAYDLFSMMDGVDKHLAVIEDERRLMGRVLEGGFIDIPYLTDH
                     EFGARLPNVTAKIKEANEMMVDALEDAKLQYKKYKSLIDKVKNDYFSREADDLKADMQ
                     QLAPRVKYLARIVKDVMSEFNRKKRSKNILDFSDYEHFALQILTNEDGSPSEIAESYR
                     QHFQEILVDEYQDTNRVQEKILSCIKTGDEHNGNLFMVGDVKQSIYKFRQADPSLFIE
                     KYQRFTIDGDGTGRRIDLSQNFRSRKEVLSTTNYIFKHMMDEQVGEVKYDEAAQLYYG
                     APYDESDHPVNLKVLVEADQEHSDLTGSEQEAHFIVEQVKDILEHQKVYDMKTGSYRS
                     ATYKDIVILERSFGQARNLQQAFKNEDIPFHVNSREGYFEQTEVRLVLSFLRAIDNPL
                     QDIYLVGLMRSVIYQFKEDELAQIRILSPNDDYFYQSIVNYINDEAADAILVDKLKMF
                     LSDIQSYQQYSKDHPVYQLIDKFYNDHYVIQYFSGLIGGRGRRANLYGLFNKAIEFEN
                     SSFRGLYQFIRFIDELIERGKDFGEENVVGPNDNVVRMMTIHSSKGLEFPFVIYSGLS
                     KDFNKRDLKQPVILNQQFGLGMDYFDVDKEMAFPSLASVAYKAVAEKELVSEEMRLVY
                     VALTRAKEQLYLIGRVKNDKSLLELEQLSISGEHIAVNERLTSPNPFHLIYSILSKHQ
                     SASIPDDLKFEKDIAQVEDSSRPNVNISIIYFEDVSTETILDNNEYRSVNQLETMQNG
                     NEDVKAQIKHQLDYQYPYVNDTKKPSKQSVSELKRQYETEESGTSYERVRQYRIGFST
                     YERPKFLSEQGKRKANEIGTLMHTVMQHLPFKKERISEVELHQYIDGLIDKHIIEADA
                     KKDIRMDEIMTFINSELYSIIAEAEQVYRELPFVVNQALVDQLPQGDEDVSIIQGMID
                     LIFVKDGVHYFVDYKTDAFNRRRGMTDEEIGTQLKNKYKIQMKYYQNTLQTILNKEVK
                     GYLYFFKFGTLQL"
     gene            17937..18839
                     /locus_tag="PPY92_000625"
     CDS             17937..18839
                     /locus_tag="PPY92_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDJ2624648.1"
                     /translation="MKFLSFKYNDKTSYGVKVKREDAVWDLTQVFADFAEGDFHPKTL
                     LAGLQQNHTLDFQEQVRKAVVAAEDSGKAEDYKISFNDIEFLPPVTPPNNVIAFGRNY
                     KDHANELNHEVEKLYVFTKAASSLTGDNATIPNHKDITDQLDYEGELGIVIGKSGEKI
                     PKALALDYVYGYTIINDITDRKAQSEQDQAFLSKSLTGGCPMGPYIVTKDELPLPENV
                     NIVTKVNNEIRQDGNTGEMILKIDELIEEISKYVALHPGDIIATGTPAGVGAGMQPPK
                     FLQPGDEVKVTIDNIGTLTTYIAK"
BASE COUNT         6661 a         2812 c         3458 g         6165 t
ORIGIN      
        1 taaatgtaag ggttttcaaa atagacaaat ttaatgaaac ttagtgatta tttcatcttt
       61 tttggtggaa aagtatctgc tttgaaaact aatgtaatta tttggataat gtttcatgta
      121 taatagttat aaatgatgaa aaggggagat atgatgacta aatctgaaaa aattattgag
      181 ttaacaaatc attacggagc acataattat ttaccattgc caattgtcat ttcagaagct
      241 gaaggggtat gggttaaaga tcctgaaggc aataaatata tggatatgtt atctgcatat
      301 tccgctgtta accaaggtca tagacatccg aaaattattc aagcattaaa agatcaagct
      361 gataaagtga ctctagtttc acgtgctttt catagtgata acttaggtga atggtacgaa
      421 aaaatttgta aactggcagg taaagataaa gctttaccaa tgaatacagg tgctgaagca
      481 gtagaaacag ctttgaaagc agcacgacgc tgggcatacg atgttaaagg aattgagcca
      541 aataaagcag aaatcattgc atttaatggt aacttccatg gtcgaacaat ggcgccagtt
      601 tcattatctt cagaagcaga ataccaacgt ggttatggtc cgttattaga tggatttaga
      661 aaagttgatt ttggagatgt agatgcattg aaagctgcaa ttaatgaaaa tactgcagca
      721 gttttagtag aaccaattca aggtgaagcg ggtataaata taccgccaga aggatatttg
      781 aaagcaatta gagaattatg tgatgaacat aatgtcttat ttattgctga cgaaatccaa
      841 gcaggattag gtcgttcggg taaattattt gctacggatt gggataatgt aaaacctgat
      901 gtctatattt taggtaaagc actaggtggt ggtgtcttcc caatttctgt tgtattagca
      961 gataaagaag tattagatgt ctttacacct ggctcacatg gttcaacatt tggtggtaat
     1021 ccacttgctt gtgctgcatc aattgctgca ttagatgtta tcgttgatga ggatttacca
     1081 gggcgctctt tagaattagg agattatttt aaagaacaat taaagcaaat tgatcatcca
     1141 tcaattaaag aagtccgtgg acgtggtttg tttataggtg tggaacttaa tgaaagtgct
     1201 agaccatatt gtgaagcttt gaaagaagaa ggcttattat gtaaagaaac gcatgatact
     1261 gtcattcgtt ttgcaccacc attaattatt actaaagaag aattggacct tgcacttgaa
     1321 aaaataagac atgtatttca ataatgtcaa aaagttgtaa caatcagttt gtaagggctt
     1381 taaaaaggaa tacgttatgt tacaatatgt ttgaaagcga aatcattagg taaagaagag
     1441 gcgaaaagga tcatgactga gaacaataat ttagtaactt ctactcaagg aattattaaa
     1501 gaagcattgc ataaattggg atttgacgaa ggaatgtacg atttaattaa agaaccttta
     1561 agaatgttac aagtgcgtat ccctgtacga atggatgatg gcacagttaa aacattcaca
     1621 ggttaccgtg cgcaacataa tgatgctgtt ggaccaacaa aagggggcgt gcgtttccac
     1681 ccagatgttg atgaagaaga agtaaaagca ttatcaatgt ggatgacttt gaaatgtggc
     1741 attgtaaact taccatacgg tggtggtaag ggtggtatcg tttgtgatcc acgtcaaatg
     1801 agcattcatg aagttgaacg tttatcacgc ggatatgtaa gagcaatttc acaattcgta
     1861 ggtccgaaca aagatattcc agcaccagat gtatttacaa actcacaaat tatggcttgg
     1921 atgatggatg aatatagtgc attagataaa tttaattcac caggtttcat cacaggtaaa
     1981 ccaattgtat tgggtggttc tcatggacgc gacagatcaa ctgcactagg tgtagttatt
     2041 gcaattgaac aagctgcaaa acgtcgtaat atgcaaattg aaggtgccaa ggttgttatt
     2101 caaggtttcg gtaatgccgg aagtttctta gctaaattct tatatgattt aggtgcaaaa
     2161 attgtaggta tctctgatgc ttacggtgca ttacacgatc caaatggctt agatatagat
     2221 tatttattag accgtcgtga tagttttggt acggtaacaa atttatttga agaaacaatc
     2281 tcaaataaag aattgtttga attagattgt gacattttag taccagcggc tatttcaaac
     2341 caaattacag aagacaatgc acatgatatt aaagctagta tcgttgttga agctgctaat
     2401 ggacctacaa caccagaagc aacacgtatt ttaactgaac gtggtatatt attagttcca
     2461 gacgtattag caagtgctgg tggtgtaacg gtttcttact tcgaatgggt acaaaataat
     2521 caaggttatt attggtctga agaagaagta aatgaaaagc tacgtgaaaa attagaagcg
     2581 gcatttgaca cgatttacga attgtctcaa aatcgaaaaa tagatatgag acttgcagca
     2641 tatatcatag gtattaaacg tacagcagaa gcagctagat atcgtggttg ggcataatta
     2701 attatcatat gtgatttaac gagcttggga cagaaaacaa agccctaagc tcgttaattt
     2761 tattttagca gcagttgact gtaaaacaat gcccgtgtaa cacgctcttt tcaaaaatag
     2821 tcggggcccc aacacagagg ctggtggaaa gtcagcttac aatagtgtgc aagttggctg
     2881 gcccccaaca ctgagaattt ctaattaaac gctcaactat atatagttga tcaaaaagac
     2941 agcagtagga taatttcaat ttggaaatat cttactgctg ttttttatag taatagctat
     3001 atttttgtat ttatgttttc tagtttaaca atactactta atgacttctt tatatttatc
     3061 agcgaaattt gtaaagacac catcaacgcc atatttattt aatcgtaaca tatcagcttt
     3121 ttcattcact gtataaggat gtactataaa tcctaagtct tttaaatgat gggtattttg
     3181 ttcagttaaa tctgtataat caggacctaa tccaatcgca taagagcgta tctcttttaa
     3241 gcgttggtcg ttaaattgtt gtagttcacc tttatcaact aattttacta atggcacatg
     3301 cttattttga cgatgaattt tctttaaact ttcgtcagaa aatgattgaa tcattacatg
     3361 tccatttttt aatttattgt tatttaaaag gtgatgcttt ttcaatgaag ctaataattg
     3421 ttcttccatt cctgggtata catcaggtga ctttgtttca atataatagt ttgcattcgg
     3481 gccataacgt tctaaaattt catctaaagt gggtacttta gcatttttat aacttgctct
     3541 tgcgtatttt ggatattttt tattaaacca acttcctgca tctaactgtt ttaattcatc
     3601 aagggtataa tcctcaactt taccgtgtcc atttgttgta cggttaacag tttcatcatg
     3661 catagcaact aaatgtccat ctttggtacg ttgtaaatca atttcgatat aagatgcttt
     3721 taactcatta tgactcttat catatgcttg aaacgtatgc tctggtgcat agccacttgc
     3781 gccacgatgt gcgatagtag tgaatcgctc attcgttaaa tttgtatgcc attgaatagc
     3841 ctgaggttta tttgcaattt gatttgtttg ttcagcgcca gcagtaggta ctgataaaaa
     3901 tcccatagta aaaacagcag aagcagccat aaatttagtg aagcttttcg aagagttagt
     3961 catagtcttt atcctttcat ctctttattt gtagtctaac aattttaaaa tactacaaaa
     4021 acctgcttgt gaaaacgctt tacacatttc ttttacaatt tataacattt ctgtaatata
     4081 aatttaaaag aattatcaaa cattttagga atcttaaaaa tgtgatgttc aacaagacga
     4141 agagagcatt aaaataccca caatgtaatt ttaatagaca ttgtgggtag gtagattaac
     4201 gtatttattg tgatagtaat tgtttagcaa catcaagttg ttgtttgacc gatgattgcc
     4261 ccgttgaccc gtaactttga cgtcgtttca agcaattttc aggctgcaaa taatcgtaaa
     4321 tatcggcatc gatactagaa tgatgttgtt gatatgttgc taaaggaaca tctaataaat
     4381 aatgaccttg ttgtatacat tctaagacga tttttcctac aatttcatgt gcagttctaa
     4441 atggaatatt tttagttact aaataatctg ctagttccgt tgcatttgaa aaatcttctt
     4501 taacagtttg attgagtcgt tctttattaa ttgtcatcgt ttgaatcata ccttcgaaaa
     4561 tacgtaaaga acctttaatt gtatggacag catcgaataa accttcttta tcttcctgca
     4621 tatctttgtt atatgctaga ggtaatcctt ttaaagtcat aagcatgctc attaaatgac
     4681 cagtcgttcg accaacttta cctctaatta attctgccat atcaggattt ttcttttgtg
     4741 gcataataga tgagccagtt gaaaatgcat ctgataatgt aatgaattta gcttcgtctg
     4801 tggaccagaa aataatttcc tctgcaaagc gtgataagtg aaccatcgtt aaagaaatat
     4861 tatgcaatgt ttcaataata tagtctctgt cactaacagc atctaggcta ttctcataga
     4921 gactgccaaa gttcaacaat gctgttgtct cgtgtctatc gatagggtat gtggtaccac
     4981 ttaaggctgc tgcacctaaa ggattaatat cgattcgttt taaactatct tcaaatcgtt
     5041 gttggtctcg ttgtaacatc caaaaataag tcataatatg atgtgcaaat gaaattggct
     5101 gtgcacgctg taaatgagta taaccaggca taattgtatc aacattattg gaagcgatgt
     5161 ctacaattac actttgtaac gacttaatta atgcgatgat atcttgcact tgtttcttag
     5221 tgtacaagtg catatctgtt gcaacttgat cgtttctact gcgtccagta tgcaacttac
     5281 caccagcatc accaatacgt ttaattaatt catgttcaat atttaaatga atatcttcta
     5341 atgatgcact aaattgaatt tgatcttgat gataatcatg ttgaatagat tttagtcctt
     5401 gtataatttg ttcgctgtct tgttgactaa taatgccttg attcgcaagc atagttgcat
     5461 gcgcaatgct gccttcgata tcttgatcta tgagcgtttg gtcaaaagta atggatgcgt
     5521 taaagtcgtc aacccactct tcaggttgta cttcaaatct accgccccaa gctttattgc
     5581 tcattgctat agcctccatg caacatcgca tttacttgag taggtaaacc atagatatcg
     5641 ataaagccaa cagcagcgtc ttgattaaat gcatcttctt ttgtataagt tgctaatttt
     5701 tcatcatata atgtgtaagg tgattttcta ccattcacga tggcattacc tttgaataat
     5761 ttaattctga catcaccact tacgtattgc tgagtactat caataaataa tttcaagcta
     5821 tcagttaaag gtgagaacca aagtccattg tatagttgtt cagcaaattg cttctcaatg
     5881 attggtttaa agtgtgcgac atctttagtt aacgtaatcg tttctaatgc tttatgcgct
     5941 tttaaaataa cttctgcagc aggtgcctca taaatttctc ttgatttgat acctacaagt
     6001 ctattttcta catggtcaat tcttccgata ccatgcttac cagctaatgc attcaacgtt
     6061 aaaattaaat cgtctaattc atatgttttg ccatcaattt gaactgggat gcctttatca
     6121 aacgttaaaa tgatttcatc agcagtatct ggtgtttctt ctaaagcatt tgttagatcg
     6181 aacgcatcct ctggtggcgc agcataagga tcttctaaaa taccacattc attcgctctg
     6241 ccccatagat tttgatcgat agaataaggt gaatcatggt tgattgatac agggatatta
     6301 tgtttaattg cataatcgat ttcttcttca cgactccatg cccactcacg tacaggtgcg
     6361 aatgctttca atgatgggtt taatgcttta atggcaactt cgaaacgtac ttggtcattc
     6421 cctttaccag tacaaccatg tgcaatacct actgaatttg ttttctcagc aatctctact
     6481 aattttttag cgattaatgg tcttgataaa gctgaaacta atggatatgc attttcatac
     6541 attaaatttc ctttgattgc ataacttaca tactcatcac taaattcttt tgttgcatca
     6601 ataatatgac attcaactgc tcccatatct aaagcttttt tataaacgat gtctaaatct
     6661 ttaccttcac caacatctag gcaacaagct acaacgtcgt atcctttgtc gataagccat
     6721 tgaacggcca cacttgtatc tagtcctcct gaatatgcta aaacaatttt ctctttcata
     6781 aaattcacct cattgtagtt atctgaatat ttcgtatgaa cacatgatag caaactttta
     6841 atgaaaataa aaccattatt ttacatattt attcataaaa tgcgatagag atagttagag
     6901 ttgtaagtat aaatgcttat ttatcaatgt ttaaaagtat tttaaaaaac atgcaaattg
     6961 tataaaaaat gtttgagtgt ttgacgccag tttgaaatgt ttgttgaaaa ataggtgtga
     7021 aacgtcacat aaatatcaat tttattgatt aagtacaaac ttgtgaattg aatcgcaaac
     7081 gtaaacttag taaaattaag atattaaatg aaacaggagg cttttataga tgactcatat
     7141 tcaattagat tttagtaaaa cgttagaatt tttcggtgaa cacgaattaa aacaacaaca
     7201 agaaattgtt aaatcaattc acaaaacaat tcatgaaggt actggtgcag gtagtgactt
     7261 cttaggctgg gttgatttac cagttgatta cgacaaagaa gaattttcaa gaattgttga
     7321 agcatcaaaa cgcattaaag aaaattctga tgttttagta gtcatcggta ttggtggttc
     7381 ttacttaggt gcacgtgcag caatcgaaat gttaacgtca tcatttagaa acagcaatga
     7441 ataccctgaa attgtatttg ttggtaatca cttatcatca acatatacga aagagttagt
     7501 tgattattta gcagacaaag atttctctgt aaacgttatt tctaaatctg gtacaactac
     7561 agaaccagca gttgcattta gattgttcaa acaattagtt gaagaaagat acggtaaaga
     7621 agaagcacaa aaacgtatat ttgcaacaac ggataaagaa aaaggtgctt taaaacagtt
     7681 ggctacaaac gaaggttatg aaacgtttat cgtacctgat gatgtaggtg gaagatattc
     7741 tgttttaaca gcagtaggat tattaccaat tgcaacagct ggaattaaca tcgaagctat
     7801 gatgattggt gctgcaaaag cacgtgaaga attatcttca gataaattag aagacaacat
     7861 tgcataccaa tatgcgacaa ttcgaaacat tttatatgca aaaggttata caacagaaat
     7921 gttgattaac tatgaaccat ctatgcaata ctttaatgaa tggtggaaac aattatttgg
     7981 tgaatcagaa ggtaaagact tcaaaggtat ctatccttca agtgccaact acacaactga
     8041 tttacattct ttaggtcaat atgtacaaga aggccgtcgt ttcttattcg aaacagtggt
     8101 aaaagtaaat catcctaaat atgatattac tattgaaaaa gatagtgatg atctagacgg
     8161 attaaattat ttggctggta aaacaatcga cgaagttaac acaaaagcat tcgaaggtac
     8221 attattagcg catactgatg gtggtgttcc taacatggta gtgaacattc cacaattaga
     8281 tgaagaaact ttcggttatg tcgtatactt cttcgaactt gcttgtgcaa tgagtggata
     8341 ccaattaggt gtaaatccat ttaaccaacc tggtgtagaa gcatataaac aaaacatgtt
     8401 cgcattatta ggtaaacctg gttttgaaga cttgaaaaaa gaattagaag agcgtttata
     8461 aaatacatta cttcaatgat tagtgaagtt tgaaaagata gaactagacg ttaactattt
     8521 aaagcatatt ttcgaggttg tcattacaaa tgtaaaaatg taatgacaac ctcgttttta
     8581 tttatatgca agaactaggt tactagctaa tgtgacaaga tgttaagaga aaattaaaga
     8641 aaaaataaca tctgtcatac aataatattg ttatactact agagactgat ttattagcat
     8701 gattacatgt taatgtttct ttacttagta attaacttta taatgtaaga ataattatct
     8761 tcaaccaaag aaagggattg atgatttgtc gtttcatcaa gtagaagaat ggtttgagat
     8821 atttcgacag tttggttatt tacctggatt tatattgtta tatattagag cgataattcc
     8881 agtatttcct ttagcactct atattttaat taacattcaa gcttatggac ctattttagg
     8941 tatattgatt agttggcttg gattaatttc tggaacattt acagtctatt tgatctgtaa
     9001 acgattggtg aacactgaga ggatgcagcg aattaaacaa cgtactgctg ttcaacgctt
     9061 gattagtttt attgatcgcc aaggattaat cccattgttt attttacttt gttttccttt
     9121 tacgccaaat acattaataa attttgtagc gagtctatct catattagac ctaaatatta
     9181 tttcattgtt ttggcatcat caaagttagt ttcaacaatt attttaggtt atttaggtaa
     9241 ggaaattact acaattttaa cgcatccttt aagagggata ttaatgttag ttgtgttggt
     9301 tgtattttgg attgttggaa aaaagttaga acagcatttt atgggatcga aaaaggagtg
     9361 acatcgtgaa aaaagttgta aaatatttga tttcattgat acttgctatt atcattgtac
     9421 tgttcgtaca aacttttgta atagttggtc atgtcattcc gaataatgat atgtcaccaa
     9481 cccttaacaa aggggatcgt gttattgtaa ataaaattaa agttacattt aatcaattga
     9541 ataatggtga tatcattaca tataggcgtg gtaacgagat atatactagt cgaattattg
     9601 ccaaacctgg tcaatcaatg gcgtttcgtc agggacaatt ataccgtgat gaccgaccgg
     9661 ttgacgcatc ttatgccaag aacagaaaaa ttaaagattt tagtttgcgc aattttaaag
     9721 aattagatgg agatattata ccgcctaaca attttgttgt gctaaatgat catgataaca
     9781 atcagcatga ttctagacaa tttggtttaa ttgataaaaa ggatattatt ggtaatataa
     9841 gtttgagata ttatcctttt tcaaaatgga cgattcagtt caaatcttaa aaagaggtgt
     9901 caaaattgaa aaaagaatta ttggaatgga ttatttcaat tgcagtcgct tttgtcattt
     9961 tatttatagt aggtaaattt attgttacac catatacaat taaaggtgaa tcaatggatc
    10021 caactttgaa agatggcgag cgagtagctg taaacattat tggatataaa acaggtggtt
    10081 tggaaaaagg taatgtagtt gtcttccatg caaacaaaaa tgatgactat gttaaacgtg
    10141 tcatcggtgt tcctggtgat aaagtagaat ataaaaatga tacattatat gtcaatggta
    10201 aaaaacaaga tgaaccatat ttaaactata atttaaaaca taaacaaggt gattacatta
    10261 ctgggacttt ccaagttaaa gatttaccga atgcgaatcc taaatcaaat gtcattccaa
    10321 aaggtaaata tttagttctt ggagataatc gtgaagtaag taaagatagc cgtgcgtttg
    10381 gcctcattga tgaagaccaa attgttggta aagtttcatt tagattctgg ccatttagtg
    10441 aatttaaaca taatttcaat cctgaaaata ctaaaaatta atatgaaaca aatacaacat
    10501 cgtttgtcgg ttttaatact gataaacgat gttttatttt gttagtacca caataaaagc
    10561 taagttcgaa atgaacttat aataaatcaa tcacaatcac tttgtgttaa aatatgtgtc
    10621 aaaggaagtg agggtttgtc atgacattac atgcttattt aggtagagcg ggaacaggta
    10681 agtctacgaa aatgttgacc gaaataaaac aaaaaatgaa agcagatccg cttggagatc
    10741 caatcatttt aattgcgcca actcaaagta catttcaatt agaacaagcc tttgtcaatg
    10801 atccggaatt aaatggtagt ttaagaacag aagtgttgca ttttgaacga ttaagtcatc
    10861 gtattttcca agaagttggt agttatagcg aacaaaagtt atctaaagct gcaacggaaa
    10921 tgatgattta taacattgtt caagaacaac aaaagtattt aaaactttat caatcacaag
    10981 caaaatatta tgggtttagt gaaaaattaa cagaacaaat tcaagatttt aaaaaatatg
    11041 cagtaacgcc tgaacattta gaacacttta ttgctgataa aaatatgcaa actcgaacta
    11101 aaaataagtt agaggatatt gctttaatat accgtgagtt cgaacaacgc attcaaaacg
    11161 agtttattac tggtgaggat tcattacaat attttattga ttgtatgccg aaatcagagt
    11221 ggctaaaacg tgctgatata tatattgatg gttttcacaa cttttcaacg attgagtatt
    11281 taataatcaa aggattaatt aaatatgcga agagtgtcac aattatattg acgacagatg
    11341 gtaaccacga tcaatttagt ttatttagaa aaccatcgga agtgttacga catattgaag
    11401 aaatagcaaa tgaactcaat atttctattg aacgtcaata tttcaaccaa ttatatcgct
    11461 tcaataatca agatttaaag catcttgaac aagaatttga tgtacttcaa atcaatcgag
    11521 tggcatgtca aggtcatatc aatattttag aatctgcgac tatgagagag gaaataaatg
    11581 aaattgcgcg acgtatcatc gttgatattc gtgataagca attacgatat caagatattg
    11641 caattttata tcgtgacgag tcttatgctt atttatttga ttccatatta ccgctttata
    11701 atattcctta taacattgat acaaagcgtt cgatgacaca tcatccggtc atggaaatga
    11761 ttcgttcatt gattgaagtt attcaatcta attggcaagt gaatccaatg ctacgcttat
    11821 tgaagactga tgtgttaacg gcatcatatc taaaaagtgc atacttagtt gatttacttg
    11881 aaaattttgt acttgaacgt ggtatatacg gtaaacgttg gttagatgat gagctattta
    11941 atgtcgaaca ttttagcaaa atggggcgta aagcgcataa actgaccgaa gatgaacgta
    12001 acacatttga acaagtcgtt aagttaaaga aagatgtcat tgataaaatt ttacattttg
    12061 aaaagcaaat gtcacaagcg gaaactgtaa aagactttgc aactgctttt tatgaaagta
    12121 tggaatattt cgaactgcca aatcaattga tgacagagcg agatgaactt gatttaaatg
    12181 gtaatcatga aaaggcggag gaaattgatc aaatatggaa tggcttaatt caaatccttg
    12241 acgacttagt tctagtattt ggagatgaac caatgtcgat ggaacgtttc ttagaagtat
    12301 ttgatattgg tttagaacaa ttagaatttg tcatgattcc acaaacatta gatcaagtta
    12361 gtattggtac gatggatttg gctaaagtcg acaataagca acatgtttac ttagttggaa
    12421 tgaacgacgg caccatgcca caaccagtaa ctgcatcaag tttaattact gatgaagaaa
    12481 agaaatattt tgaacaacaa gcaaatgtag agttgagtcc tacatcagat attttacaga
    12541 tggatgaagc atttgtttgc tatgttgcta tgactagagc taagggagat gttacatttt
    12601 cttacagtct aatgggatca agtggtgatg ataaggagat cagcccattt ttaaatcaaa
    12661 ttcaatcatt gttcaaccaa ttggaaatta ctaacattcc tcaataccat gaagttaacc
    12721 cattgtcact aatgcaacat gctaagcaaa ccaaaattac attatttgaa gcattgcgtg
    12781 cttggttaga tgatgaaatt gtggctgata gttggttaga tgcttatcaa gtaattagag
    12841 atagcgatca tttaaatcaa ggtttagatt atttaatgtc agcattaacg tttgacaatg
    12901 aaactgtaaa attaggtgaa acgttgtcta aagatttata tggtaaggaa atcaatgcca
    12961 gtgtatctcg ttttgaaggt tatcaacaat gcccatttaa acactatgct tcacatggtc
    13021 tgaaactaaa tgaacgaacg aaatatgaac ttcaaaactt tgatttaggt gatattttcc
    13081 attccgtttt aaaatatata tctgaacgta ttaatggcga ttttaaacaa ttagacctga
    13141 aaaaaataag acaattaacg aatgaagcat tggaagaaat tttacctaaa gttcagttta
    13201 atttattaaa ttcttcagct tactatcgtt atttatcaag acgcattggc gctattgtag
    13261 aaacaacact aagcgcatta aaatatcaag gcacgtattc aaagtttatg ccaaaacatt
    13321 ttgagacaag ttttagaagg aaaccaagaa caaatgacga attaattgca caaacattaa
    13381 cgacaactca aggtattcca attaatatta gagggcaaat tgaccgtatc gatacgtata
    13441 caaagaatga tacaagtttt gttaatatca ttgactataa atcctctgaa ggtagtgcga
    13501 cacttgattt aacgaaagta tattatggta tgcaaatgca aatgatgaca tacatggata
    13561 tcgttttaca aaataaacaa cgccttggat taacagatat tgtgaaacca ggtggattat
    13621 tatacttcca tgtacatgaa cctagaatta aatttaaatc atggtctgat attgatgaag
    13681 ataaactaga acaagattta attaaaaagt ttaagctgag tggtttagtg aatgcagacc
    13741 aaactgttat tgatgcattg gatattcgtt tagaacctaa attcacttca gatattgtac
    13801 cagttggttt gaataaagat ggctctttga gtaaacgagg cagccaagtg gcagatgaag
    13861 caacaattta taaattcatt cagcataaca aagagaattt tatagaaaca gcttcaaata
    13921 ttatggatgg acatactgaa gttgcaccat taaagtacaa acaaaaattg ccatgtgctt
    13981 tttgtagtta tcaatcggta tgtcatgtag atggcatgat tgatagtaag cgatatcgaa
    14041 ctgtagatga aacaataaat ccaattgaag caattcaaaa tattaacatt aatgatgaat
    14101 ttgggggtga gtaatagatg acaattccag agaaaccaca aggcgtgatt tggactgacg
    14161 cgcaatggca aagtatttac gcaactggac aagatgtact tgttgcagcc gcggcaggtt
    14221 caggtaaaac agctgtacta gttgagcgta ttatccaaaa gattttacgt gatggcattg
    14281 atgtcgatcg acttttagtc gtaacgttta caaacttaag cgcacgtgaa atgaagcatc
    14341 gtgtagacca acgtattcaa gaggcatcga ttgctgatcc tgcaaatgca cacttgaaaa
    14401 accaacgcat caaaattcat caagcacaaa tatctacact tcatagtttt tgcttgaaat
    14461 taattcaaca gcattatgat gtattaaata ttgacccgaa ctttagaaca agcagtgaag
    14521 ctgaaaatat tttattatta gaacaaacga tagatgaggt catagaacaa cattacgata
    14581 tccttgatcc tgcttttatt gaattaacag aacaattgtc ttcagataga agtgatgatc
    14641 agtttcgaat gattattaaa caattgtatt tctttagcgt tgcaaatcca aatcctacaa
    14701 attggttgga tcaattggtg acaccatacg aagaagaagc acaacaagcg caacttattc
    14761 aactactaac agacttatct aaagtattta tcacagctgc ctatgatgct ttaaataagg
    14821 cgtatgattt gtttagtatg atggatggcg tcgataaaca tttagctgtt atagaagatg
    14881 aacgacgttt aatggggcgt gttttagaag gtggttttat tgatatacct tatttaactg
    14941 atcacgaatt tggcgcgcgt ttgcctaatg taacagcgaa aattaaagaa gcaaatgaaa
    15001 tgatggtcga tgccttagaa gatgctaaac ttcagtataa aaaatataaa tcattaattg
    15061 ataaagtgaa aaatgattac ttttcaagag aagctgatga tttgaaagct gatatgcaac
    15121 aattggcgcc acgagtaaag taccttgcgc gtattgtgaa agatgttatg tcagaattca
    15181 atcgaaaaaa gcgtagcaaa aatattctgg atttttctga ttatgaacat tttgcattac
    15241 aaattttaac taatgaggat ggttcgcctt cagaaattgc cgaatcatac cgtcaacact
    15301 ttcaagaaat attggtcgat gagtatcaag atacgaaccg ggttcaagag aaaatactat
    15361 cttgcatcaa aacgggtgat gaacataatg gtaatttatt tatggttgga gatgttaagc
    15421 aatccattta taaatttaga caagctgatc caagtttatt tattgaaaag tatcaacgct
    15481 ttactataga tggagatggc actggacgtc gaattgattt gtcgcaaaac ttccgttctc
    15541 gaaaagaagt actgtcaacg actaactata tattcaaaca tatgatggat gaacaagtcg
    15601 gtgaagtaaa atatgatgaa gcggcacagt tgtattatgg tgcaccatat gatgaatcgg
    15661 accatccagt aaacttaaaa gtgcttgttg aagcggatca agaacatagt gatttaactg
    15721 gtagtgaaca agaagcgcat tttatagtag aacaagttaa agatatctta gaacatcaaa
    15781 aagtttatga tatgaaaaca ggaagctata gaagtgcgac atacaaagat atcgttattc
    15841 tagaacgcag ctttggacaa gctcgcaatt tacaacaagc ctttaaaaat gaagatattc
    15901 cattccatgt gaatagtcgt gaaggttact ttgaacaaac agaagtccgc ttagtattat
    15961 catttttaag agcgatagat aatccattac aagatattta tttagttggg ttaatgcgct
    16021 ccgttatata tcagttcaaa gaagacgaat tagctcaaat tagaatattg agtccaaatg
    16081 atgactactt ctatcaatcg attgtaaatt acattaatga cgaagcagca gatgctattt
    16141 tagttgataa attaaaaatg tttttatcag atattcaaag ttaccaacaa tatagtaaag
    16201 atcatccggt gtatcagtta attgataaat tttataatga tcattatgtt attcaatact
    16261 ttagtggact tattggtgga cgtggacgac gtgcaaacct ttatggttta tttaataaag
    16321 ctatcgagtt tgagaattca agttttagag gtttatatca atttattcgt tttatcgatg
    16381 aattgattga aagaggcaaa gattttggtg aggaaaatgt agttggtcca aacgataatg
    16441 ttgttagaat gatgacaatt catagtagta aaggtctaga gtttccattt gtcatttatt
    16501 ctggattgtc aaaagatttt aataaacgtg atttgaaaca accagttatt ttaaatcagc
    16561 aatttggtct cggaatggat tattttgatg tggataaaga aatggcattt ccatctttag
    16621 cttcggttgc atataaagct gttgccgaaa aagaacttgt gtcagaagaa atgcgattag
    16681 tctatgtagc attaacaaga gcgaaagaac aactttattt aattggtaga gtgaaaaatg
    16741 ataaatcgtt actagaacta gagcaattgt ctatttctgg tgagcacatt gctgtcaatg
    16801 aacgattaac ttcaccaaat ccgttccatc ttatttatag tattttatct aaacatcaat
    16861 ctgcgtcaat tccagatgat ttaaaatttg aaaaagatat agcacaagtt gaagatagta
    16921 gtcgtccgaa tgtaaatatt tcaattatat actttgaaga tgtgtctaca gaaaccattt
    16981 tagataataa tgaatatcgt tcggttaatc aattagaaac tatgcaaaat ggtaatgagg
    17041 atgttaaagc acaaattaaa caccaacttg attatcaata tccatatgta aatgatacta
    17101 aaaagccatc aaaacaatct gtttctgaat tgaaaaggca atatgaaaca gaagaaagtg
    17161 gcacaagtta cgaacgagta agacaatatc gtatcggttt ttcaacgtat gaacgaccta
    17221 aatttctaag tgaacaaggt aaacgaaaag cgaatgaaat tggtacgtta atgcatacag
    17281 tgatgcaaca tttaccattc aaaaaagaac gcatatctga agttgagtta catcagtata
    17341 tcgatggatt aatcgataaa catattatcg aagcagatgc gaaaaaagat atccgtatgg
    17401 atgaaataat gacatttatc aatagtgagt tatattcgat tattgctgaa gcagagcaag
    17461 tttatcgtga attaccgttt gtagttaacc aagcattagt tgaccaattg ccacaaggag
    17521 acgaagacgt ctcaattatt caaggtatga ttgacttaat ctttgttaaa gatggtgtgc
    17581 attattttgt agactataaa accgatgcat ttaatcgtcg ccgtgggatg acagatgaag
    17641 aaattggtac acaattaaaa aataaatata agatacagat gaaatattat caaaatacgc
    17701 ttcaaacgat acttaataaa gaagttaaag gttatttata cttcttcaaa tttggtacat
    17761 tgcaactgta gtattttgat tttcaaaaga ataaaaaata atttcgatta agtgcaaagt
    17821 ccttgtagca gaatgaacac aactcatttt caaaattgtc ttacttattt atttgttatt
    17881 tgataacgaa aaaagttata atgtgaatta agataaagat gaggagttga gaatgaatga
    17941 aattcttatc attcaagtat aatgacaaaa cttcatatgg cgttaaagta aaacgcgaag
    18001 atgctgtatg ggatttaaca caagtatttg ctgactttgc agaaggagat ttccatccta
    18061 aaacattgtt agctggttta caacaaaatc atactttaga ttttcaagaa caagtacgta
    18121 aagcagttgt agcagcagaa gatagcggca aagctgaaga ctataaaatt tcatttaatg
    18181 acattgaatt cttaccacca gtaacacctc cgaataatgt gattgctttt ggtagaaatt
    18241 acaaagatca tgcgaacgaa ttaaatcatg aagtagaaaa attatatgta tttacaaaag
    18301 cagcgtcatc tttaacagga gataatgcaa caattccaaa tcataaagat attactgatc
    18361 aattagatta tgaaggtgaa ttaggtattg ttattggtaa gtctggtgaa aagattccaa
    18421 aagcattagc tttagattat gtttacggct atacaattat taacgatatc actgatcgca
    18481 aagcacaaag tgaacaagat caagcatttt tatcaaaaag tttaactggc ggttgcccaa
    18541 tgggtcctta tatcgttact aaagacgaac taccattacc tgaaaatgta aatattgtta
    18601 caaaagttaa caatgaaatt agacaagatg gtaacactgg cgaaatgatt cttaaaattg
    18661 atgaattaat agaagaaatt tcaaaatatg ttgcactaca tccgggagat attattgcaa
    18721 ctggtacacc agcaggcgtt ggtgcaggta tgcaaccacc taaattttta caaccaggtg
    18781 atgaagttaa agtgactatt gataatattg gaacgctgac aacttatatc gctaaataat
    18841 tatcatttaa aaagctaacc agtctttata tagattggtt agttttttct tgcttttcta
    18901 aaaaggtgtt aaagataaat tatttataat gttaccattt tgagatgaaa gtgaaatatt
    18961 gatattaaga agtagttgat tattttacag cagattcaca atattctaat aaggaataaa
    19021 atgtcatgtt cttcctctca aatatagaag tgtggtagaa tatatattcg tgtataatca
    19081 aatcatgatt aaatta
//
LOCUS       DANBWH010000026        19071 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.026, whole genome shotgun sequence.
ACCESSION   DANBWH010000026 DANBWH010000000
VERSION     DANBWH010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19071
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.026"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(1..93)
                     /locus_tag="PPY92_000626"
     CDS             complement(1..93)
                     /locus_tag="PPY92_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624649.1"
                     /translation="MLKSKFPSPLSTVDISFFEIIYIFSYQDIL"
     gene            complement(431..835)
                     /locus_tag="PPY92_000627"
     CDS             complement(431..835)
                     /locus_tag="PPY92_000627"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDJ2624650.1"
                     /translation="MNTLNLDIVTPNGSVYNRDNVELVVMQTTAGEIGVMSGHIPTVA
                     ALKTGFVKVKFHDGTEYIAVSDGFVEVRKDKVSIIVQTAETAREIDVERAKLAKARAE
                     SHLENDDDNTDIHRAERALERANNRLRVAELK"
     gene            complement(855..2267)
                     /gene="atpD"
                     /locus_tag="PPY92_000628"
     CDS             complement(855..2267)
                     /gene="atpD"
                     /locus_tag="PPY92_000628"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDJ2624651.1"
                     /translation="MGIGRVTQVMGPVIDVRFEHNEVPKINNALVIDVPKEEGTIQLT
                     LEVALQLGDDVVRTIAMDSTDGVQRGMDVKDTGKEISVPVGDETLGRVFNVLGETIDL
                     KEEISDSVRRDPIHRQAPAFDELSTEVQILETGIKVVDLLAPYIKGGKIGLFGGAGVG
                     KTVLIQELINNIAQEHGGISVFAGVGERTREGNDLYFEMSDSGVIKKTAMVFGQMNEP
                     PGARMRVALSGLTMAEYFRDEQGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTTKGSVTSIQAVFVPADDYTDPAPATAFAHLDATTNLERKLTE
                     MGIYPAVDPLASTSRALEPSIVGQEHYEVARDVQSTLQKYRELQDIIAILGMDELSDE
                     DKQTVERARRIQFFLSQNFHVAEQFTGQKGSYVPVKTTVANFKDILDGKYDHIPEDAF
                     RLVGSMDDVIAKAKDMGVEV"
     gene            complement(2289..3155)
                     /locus_tag="PPY92_000629"
     CDS             complement(2289..3155)
                     /locus_tag="PPY92_000629"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDJ2624652.1"
                     /translation="MASLKEIDTRIKSTKKMKQITKAMNMVSSSKLRRAEKNTKQFTP
                     YMDKMQDAITAVAGASSNTNHPMLRPRKITRSGYLVITSDKGLAGAYSANVLKKLITD
                     IEAKHQDSSEYSIVVLGQQGVDFLKNRGYDIEYSQVDVPDQPSFKSVQALANHAIDLY
                     SEEEIDELNIYYSHYVSVLENKPTSRQVLPLSQEDSSKGHGHLSSYEFEPDKESILSV
                     ILPQYVESLIYGTILDAKASEHATRMTAMKNATDNATELIDDLSLEYNRARQAEITQQ
                     ITEIVGGSAALE"
     gene            complement(3186..4694)
                     /locus_tag="PPY92_000630"
     CDS             complement(3186..4694)
                     /locus_tag="PPY92_000630"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDJ2624653.1"
                     /translation="MAIKAEEISALLRSQIENYESEMSVTDVGTVLQIGDGIALIHGL
                     NDVMAGELVEFHNGVLGLAQNLEESNVGVVILGPYTGITEGDEVKRTGRIMEVPVGEE
                     LIGRVVNPLGQPIDGQGPINTTKTRPVEKKATGVMDRKSVDEPLQTGIKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQGTICIYVAIGQKDSTVRANVEKLRQAGAL
                     DYTIVVAASASEPSPLLYIAPYSGVTMGEEFMFNGKHVLIVYDDLTKQAAAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLNDDLGGGSITALPIIETQAGDISAYVPTNV
                     ISITDGQIFLQSDLFFSGVRPAINAGQSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     ESFAQFGSDLDEFTASKLERGKRTVEVLKQDQNKPLPVEHQVLIIYALTKGYLDDIPV
                     VDITRFEDELNHWAESNATELLNEIRETGGLPDAEKFDTAINEFKKSFSKSE"
     gene            complement(4716..5255)
                     /locus_tag="PPY92_000631"
     CDS             complement(4716..5255)
                     /locus_tag="PPY92_000631"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDJ2624654.1"
                     /translation="MVKVANKYAKALFDVSLDTNNLETINEELTVINEAVKDKIEQLK
                     MVDSNPTQTAEQRRELINGVFTDINPYIKNMMYVLADNRHISLIADVFKAFQSLYNGH
                     YNQDFATIESTYELSQEELDKIVKLVTQQTKLSKVIVDTKINPDLIGGFRVKVGTTVL
                     DGSVRNDLVQLQRKFRRVN"
     gene            complement(5255..5776)
                     /locus_tag="PPY92_000632"
     CDS             complement(5255..5776)
                     /locus_tag="PPY92_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDJ2624655.1"
                     /translation="MTETANLFVLGAAGGVEWGTVIVQVLTFIVLLALLKKFAWGPLK
                     DVMDKRERDINRDIDDAEQAKLNAQKLEEENKQKLKETQEEVQKILEDAKVQARQQQE
                     QIIHEANVRANGMIETAQSEINSQKERAIADINNQVSELSVLIASKVLRKEISEQDQK
                     ALVDKYLKEAGDK"
     gene            complement(5974..6186)
                     /gene="atpE"
                     /locus_tag="PPY92_000633"
     CDS             complement(5974..6186)
                     /gene="atpE"
                     /locus_tag="PPY92_000633"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDJ2624656.1"
                     /translation="MNLIAAAIAIGLSALGAGIGNGLIVSRTVEGVARQPEARGQLMG
                     IMFIGVGLVEALPIIGVVIAFMTFAG"
     gene            complement(6229..6957)
                     /gene="atpB"
                     /locus_tag="PPY92_000634"
     CDS             complement(6229..6957)
                     /gene="atpB"
                     /locus_tag="PPY92_000634"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDJ2624657.1"
                     /translation="MDHKSPLVSWNLFGFDIVFNLSSILMILVTAFLVFLLAIICTRN
                     LKKRPTGKQNFVEWIFDFVRGIIEGNMAWKKGGQFHFLAVTLILYIFIANMLGLPFSI
                     VTKDHTLWWKSPTADATVTLTLSTTIILLTHFYGIKMRGTKQYLKGYVQPFWPLAIIN
                     VFEEFTSTLTLGLRLYGNIFAGEILLTLLAGLFFNEPAWGWIISIPGLIVWQAFSIFV
                     GTIQAYIFIMLSMVYMSHKVADEH"
     gene            complement(6978..7430)
                     /locus_tag="PPY92_000635"
     CDS             complement(6978..7430)
                     /locus_tag="PPY92_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HDJ2624658.1"
                     /translation="MNRIDTTNRLYYHLVCMSGTVFMIDISHTEAYKMSRFYNIFKQF
                     IQYYFYLIIILGGLYLYTHHAFILGLIIGVTGSAINTCIFECYLAKAKRPDTMHISTG
                     NMWRYLVAIIACMIWYLNKAHVSIIGIIIGLMISYVVVIIRPLLKVSK"
     gene            complement(7498..8625)
                     /gene="wecB"
                     /locus_tag="PPY92_000636"
     CDS             complement(7498..8625)
                     /gene="wecB"
                     /locus_tag="PPY92_000636"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDJ2624659.1"
                     /translation="MKKIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREML
                     DSVLSTFEIKPKYDLNIMKSGQTLSEITSKSITQLEQVIQLEKPDMVLVHGDTMTTFA
                     GGLAAFYNQVPIGHVEAGLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLSE
                     GKYSESVVVTGNTAIDAMKYTVDDNYKSNIMDKYHDKKFILMTAHRRENIGKPMENIF
                     KAVRRLIDEYTDLALVYPMHKNPKVREVAQKILGSHDRIELIEPLDVVDFHNFAKKSY
                     FILTDSGGIQEEAPSFNKPVLVLRSVTERPEGVEAGTLKVIGTNKQNVYQAAKELIDD
                     ERLYHQMSEASNPYGDGFASERIVNHIKYYLNLITEKPSDF"
     gene            complement(8649..9278)
                     /gene="upp"
                     /locus_tag="PPY92_000637"
     CDS             complement(8649..9278)
                     /gene="upp"
                     /locus_tag="PPY92_000637"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDJ2624660.1"
                     /translation="MSKVHVFDHPLIQHKLSYIRDVNTGTKEFRELVDEVGMLMAYEV
                     TRDLELQDVDIETPVTKMTAKRLAGKKLAIVPILRAGLGMTDGILSLVPAARVGHIGL
                     YRDPETLKAVEYFAKLPQDITERQIIVVDPMLATGASAIEAITSLKKRGAKNIRFMCL
                     IAAPEGVEKMHEAHPDVDIYIAALDEKLNDKAYITPGLGDAGDRLFGTK"
     gene            complement(9306..10544)
                     /locus_tag="PPY92_000638"
     CDS             complement(9306..10544)
                     /locus_tag="PPY92_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDJ2624661.1"
                     /translation="MSYITKQDKVIAEAIEREFQRQNSNIELIASENFVSEAVMEAQG
                     SVLTNKYAEGYPGRRYYGGCEFVDVTESIAIDRAKALFGAEHVNVQPHSGSQANMAVY
                     LVALEMGDTVLGMNLSHGGHLTHGAPVNFSGKFYNFVEYGVDKDTERINYDEVRKLAL
                     EHKPKLIVAGASAYSRTIDFKKFKEIADEVNAKLMVDMAHIAGLVAAGLHPNPVEYAD
                     FVTTTTHKTLRGPRGGMILCKEEYKKDIDKTIFPGIQGGPLEHVIAAKAVAFGEALEN
                     NFKTYQQQVVKNAKVLAEALINEGFRIVSGGTDNHLVAVDVKGSIGLTGKEAEETLDS
                     VGITCNKNTIPFDQEKPFVTSGIRIGTPAATTRGFDEKAFEEVAKIISLALKNSKDEE
                     KLQQAKERVAKLTAEYPLYQ"
     gene            complement(10571..11095)
                     /locus_tag="PPY92_000639"
     CDS             complement(10571..11095)
                     /locus_tag="PPY92_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HDJ2624662.1"
                     /translation="MKDLTMLLDELKEMSFFNKGDICLIGCSTSEVIGEKIGTVGSME
                     VAETIFNALDVVSKETGVTFAFQGCEHINRAITIEKSQYNPLTMEEVSVVPDVHAGGS
                     LATYAFQHMKDPIVVEHITVPCGIDIGQTLIGMHIKHVCVPVRTSVKQVGQAIVTIAT
                     SRPKKIGGERAKYK"
     gene            complement(11202..11621)
                     /locus_tag="PPY92_000640"
     CDS             complement(11202..11621)
                     /locus_tag="PPY92_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HDJ2624663.1"
                     /translation="MKILFVCTGNTCRSPLAESIAKEVMPNHQFESRGIFAVNNQGVS
                     NYVEDLVEEHHLAETTLSQQFTEADLKADIILTMSYSHKELIEAHFGLQNHVFTLHEY
                     VKEAGEVIDPYGGTKEMYVHTYEELVSLILKLKDIIC"
     gene            complement(11618..12664)
                     /locus_tag="PPY92_000641"
     CDS             complement(11618..12664)
                     /locus_tag="PPY92_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDJ2624664.1"
                     /translation="MDTKIWDVREYNEDLQQYPKINEIKDIVLNGGLIGLPTETVYGL
                     AANATDEEAVAKIYEAKGRPSDNPLIVHIHSKVQLKDFTYTLDPRVEKLMQAFWPGPI
                     SFILPLKPGYLCRKVSGGLSSVAVRMPSHSVGRQLLQIINEPLAAPSANLSGRPSPTT
                     FNHVYQDLNARIDGIVQAEQSEEGLESTVLDCTSFPYKIARPGSITAAMITEILPNSI
                     AHADYNDTEQPIAPGMKYKHYSPNTPLTIITDIESKIGNDGKDWSSIAFIVPSNKVAF
                     IPSEAQFIQLCQDDNDVKQASHNLYDVLHSLDENENISAAYIYGFELNDNTEAIMNRM
                     LKAAGNHIIKGCEL"
     gene            complement(12748..13584)
                     /gene="prmC"
                     /locus_tag="PPY92_000642"
     CDS             complement(12748..13584)
                     /gene="prmC"
                     /locus_tag="PPY92_000642"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDJ2624665.1"
                     /translation="MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHM
                     HDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLH
                     FLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQI
                     QFLTGDALKPLIKEGIKLNGLISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAI
                     YESIIGDLPHVMEKGSPVVFEIGYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSF
                     KW"
     gene            complement(13571..14647)
                     /gene="prfA"
                     /locus_tag="PPY92_000643"
     CDS             complement(13571..14647)
                     /gene="prfA"
                     /locus_tag="PPY92_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDJ2624666.1"
                     /translation="MFDQLDIVEERYEQLNELLSDPDVVNDSDKLRKYSKEQADLQKT
                     VDVYRNYKAKKEELADIEEMLSETDDKEEVEMLKEESNGIKAELPNLEEELKILLIPK
                     DPNDDKDVIVEIRAAAGGDEAAIFAGDLMRMYSKYAESQGFKTEIVEASESDHGGYKE
                     ISFSVSGNGAYSKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEVEDVEIEIRNE
                     DLKIDTYRSSGAGGQHVNTTDSAVRITHLPTGVIATSSEKSQIQNREKAMKVLKARLY
                     DMKVQEEQQKYASQRKSAVGTGDRSERIRTYNYPQSRVTDHRIGLTLQKLGQIMEGHL
                     EEIIDALTLSEQTDKLKELNNGEL"
     gene            complement(14648..15247)
                     /locus_tag="PPY92_000644"
     CDS             complement(14648..15247)
                     /locus_tag="PPY92_000644"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDJ2624667.1"
                     /translation="MYETYHSGWIECITGSMFSGKSEELIRRLRRGIYAKQKVVVFKP
                     AIDDRYHKEKVVSHNGNAIEAINISKASEIMTHNLTNVDVIGIDEVQFFDDEIVSIVE
                     KLSADGHRVIVAGLDMDFRGEPFEPMPKLMAVSEQVTKLQAVCAVCGSSSSRTQRLIN
                     GKPAKIDDPIILVGANESYEPRCRAHHIVAPSDNNKEEL"
     gene            complement(15594..15848)
                     /locus_tag="PPY92_000645"
     CDS             complement(15594..15848)
                     /locus_tag="PPY92_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDJ2624668.1"
                     /translation="MKQGIHPEYHQVIFLDTTTNFKFLSGSTKTSSEMMEWEDGKEYP
                     VIRLDISSDSHPFYTGRQKFAAADGRVERFNKKFGLKSNN"
     gene            complement(15966..17282)
                     /gene="rho"
                     /locus_tag="PPY92_000646"
     CDS             complement(15966..17282)
                     /gene="rho"
                     /locus_tag="PPY92_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDJ2624669.1"
                     /translation="MPERERTSPQYESFHELYKNYTTKELTQKAKTLKLTNYSKLNKK
                     ELVLAIMEAQMEKDGNYYMEGILDDIQPDGYGFLRTVNYSKGEKDIYISASQIRRFEI
                     KRGDKVTGKVRKPKDNEKYYGLLQVDFVNDHNAEEVKKRPHFQALTPLYPDERIKLET
                     ETQNYSTRIMDLVTPIGLGQRGLIVAPPKAGKTSLLKEIANAISTNKPDAKLFILLVG
                     ERPEEVTDLERSVEAAEVVHSTFDEPPEHHVKVAELLLERAKRLVEIGEDVIILMDSI
                     TRLARAYNLVIPPSGRTLSGGLDPASLHKPKAFFGAARNIEAGGSLTILATALVDTGS
                     RMDDMIYEEFKGTGNMELHLDRKLSERRIFPAIDIGRSSTRKEELLISKSELDTLWQL
                     RNLFTDSTDFTERFIRKLKRSKNNEDFFKQLQKSAEESTKTGRPII"
     gene            complement(17529..18956)
                     /locus_tag="PPY92_000647"
     CDS             complement(17529..18956)
                     /locus_tag="PPY92_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDJ2624670.1"
                     /translation="MRDYTKQYINGEWVESNSNETIEVINPATEEVIGKVAKGNKADV
                     DKAVEAADDVYLEFRHTSVKERQALLDKIVKEYENRKDDIVQAITDELGAPLSLSERV
                     HYQMGLNHFVAARDALDNYEFEERRGDDLVVKEAIGVSGLITPWNFPTNQTSLKLAAA
                     FAAGSPVVLKPSEETPFAAVILAEIFDKVGVPKGVFNLVNGDGAGVGNPLSEHPKVRM
                     MSFTGSGPTGSKIMEKAAKDFKKVSLELGGKSPYIVLDDVDIKEAAKATTGKVVNNTG
                     QVCTAGTRVLVPNKIKDAFLAELKEQFSQVRVGNPREDGTQVGPIISKKQFDQVQNYI
                     NKGIEEGAELFYGGPGKPEGLEKGYFARPTIFINVDNQMTIAQEEIFGPVMSVITYND
                     LDEAIQIANDTKYGLAGYVIGKDKETLHKVARSIEAGTVEINEAGRKPDLPFGGYKQS
                     GLGREWGDYGIEEFLEVKSIAGYFK"
BASE COUNT         5627 a         3840 c         2847 g         6757 t
ORIGIN      
        1 ctataaaata tcttggtatg aaaaaatgta gataatttca aaaaatgaaa tgtcgactgt
       61 agatagtggt gaaggaaatt ttgattttag caatctatta gtgattgtaa aaaagtaaaa
      121 ctattcttgg gagtcggaca taaatcaatg tctagactct atagtgttgt atttggcagt
      181 agttgacggg gccccaacat aaagaaatac tttttcttta aaaattagta tttcttatac
      241 atgagtttta ctcatgtatt cctattttta aatacacatt agctgtgttt aatgataaag
      301 aaccactaca tcataaatct ttaatggttc tttatcaatt ccttcccccc tctcatgttt
      361 tgtatcttta aatcaatata attcaaaaaa gggtcgaaga tatgaaatca catcttcgac
      421 cctttattta ctattttaat tcagccacac gcaaacggtt atttgctctc tctaaagctc
      481 tttcggctct atgaatatca gtattgtcgt catcattttc caagtgagac tctgctcttg
      541 ctttggctaa tttagctctt tcaacatcaa tttctcttgc agtttctgca gtctgaacaa
      601 tgattgaaac tttatctttt ctaacttcaa caaagccatc gcttacagca atatattcag
      661 ttccatcgtg aaatttcact tttacaaagc ctgtttttaa agcagctaca gttggaatat
      721 gtccactcat gacacctatc tcaccagctg ttgtttgcat aacaacgagt tcaacattat
      781 cacgattgta aacagaacca ttaggagtga caatatctag gtttaatgta ttcattatcc
      841 attcctccta attgttatac ttcaacaccc atatctttag cttttgcaat aacatcatcc
      901 atgctaccaa ctaaacggaa tgcatcttct ggaatatggt catatttacc atctaagata
      961 tctttaaagt ttgcaactgt tgtcttaaca ggtacataag aacctttttg accagtaaat
     1021 tgttccgcta cgtggaagtt ttgagataag aagaattgaa ttctacgtgc gcgttcaact
     1081 gtttgtttat cttcatcaga taattcgtcc atacctaaga tagcaatgat atcttgcaat
     1141 tcacggtatt tttgaagtgt tgattgtaca tcacgagcta cttcataatg ttcttgacct
     1201 acaattgatg gttccaatgc tcttgatgta gacgctaatg gatccacggc tggataaata
     1261 cccatttcag ttaatttacg ttctaagtta gtagttgcat ctaaatgggc aaacgctgtc
     1321 gcaggcgctg ggtcagtata gtcatcggca ggtacgaata ccgcttgaat agaagtaact
     1381 gatccttttg ttgtagacgt aatacgttct tgtaattgtc ccatttcagt agcaagtgtt
     1441 ggttggtaac ctactgcaga aggcatacga cctaataatg cagatacctc agaaccagct
     1501 tgtgtaaatc tgaaaatgtt atcgatgaat aataatacgt cttgaccttg ttcgtcacgg
     1561 aaatattcag ccattgttaa accagataat gcaacacgca tacgtgcacc aggtggctca
     1621 ttcatttgcc cgaataccat ggctgttttc ttaattacac cactgtcact catttcgaag
     1681 tataaatcgt taccttcacg agtacgttca cctacaccgg cgaatacaga aataccaccg
     1741 tgctcttgag cgatgttgtt aattaattct tggattaata ctgttttacc tacaccggca
     1801 ccaccgaaca atccgatttt accaccttta atataaggtg ctagtaaatc tactacttta
     1861 atacctgttt ctaaaatttg aacttctgtt gaaagttcat cgaatgctgg tgcttgacga
     1921 tggataggat cgcggcgaac agaatcacta atttcttctt taaggtcaat tgtttcacct
     1981 agtacattaa atacacgacc taatgtttcg tcaccaacag gtacactaat ttctttgcct
     2041 gtatctttta catccatgcc tctttggaca ccatcagttg aatccatcgc aattgtacga
     2101 acaacgtcgt cacctaattg cagcgcaact tctaatgtta gttgtattgt accttcttct
     2161 ttaggcacat caataaccaa ggcgttatta attttaggaa cttcgttatg ttcaaatcga
     2221 acatcaatta caggacccat aacttgagtt acacggccaa ttcccatgct attttcctcc
     2281 tttaaatatt attcaagcgc tgcggaacca ccaacaattt cagtaatttg ttgcgtaatt
     2341 tctgcttgtc tcgctctgtt atattctaat gataagtcat caataagttc agttgcatta
     2401 tcagtggcat ttttcatcgc agtcatacgt gttgcatgct cacttgcttt tgcgtctaat
     2461 attgttccgt aaatcaaact ctcaacatat tgaggcaaga ttacacttaa gatagattct
     2521 ttatctggct caaattcata agaagacaaa tgaccatgcc ccttactaga atcctcttga
     2581 gataatggta atacttgtct agatgtaggc ttgttttcaa gaacgctgac ataatgacta
     2641 tagtatatat ttaattcatc aatttcttct tcactgtata agtctatagc atggttagct
     2701 agtgcttgaa cagatttgaa agaaggttga tcaggtacgt ctacttgaga atactcaatg
     2761 tcataacctc tattttttag gaaatcaaca ccttgttgcc ctaaaactac aatactgtat
     2821 tcgctactat cttgatgttt cgcttcaata tcagtaatca attttttaag cacgtttgca
     2881 ctatatgcac ctgctaaacc tttatcactc gtgataacta aatatccact tctagtaatt
     2941 ttacgaggtc ttagcattgg atggtttgta ttgctacttg cccctgcaac tgcagtaatt
     3001 gcatcttgca ttttatccat atatggtgtg aattgttttg tatttttttc agctctacga
     3061 agttttgaac ttgataccat gttcatcgct ttcgtaatct gcttcatttt tttggttgat
     3121 tttattcgag tatctatttc tttaagagaa gccactatct caccacctta tactaaactt
     3181 gtttattatt cagatttgct aaagcttttt ttgaattcgt taattgctgt gtcaaacttc
     3241 tcagcatctg gtaagccacc agtttctctg atttcattta acagttcagt agcatttgat
     3301 tctgcccagt ggtttaactc gtcttcaaaa cgtgtgatat ctacaactgg aatatcatct
     3361 aaatatcctt ttgttaatgc ataaataatc aacacttggt gttcgactgg taatggtttg
     3421 ttttgatctt gttttaagac ttcaacggta cgtttaccac gttctaattt acttgcagta
     3481 aattcatcaa ggtctgaacc gaattgtgca aatgattcaa gttctctgta tgacgctaag
     3541 tcaagacgta acgtaccagc aactttcttc attgctttaa tttgtgcaga tccaccaaca
     3601 cgagatacag attgtccggc attaatcgct ggtcttacac ctgagaagaa taaatcagat
     3661 tgtaagaaga tttgtccatc tgtaattgaa ataacgtttg ttggtacata agctgaaata
     3721 tcaccagctt gtgtttcgat aattggtaat gcagtaattg aaccgccacc taagtcatcg
     3781 tttaattttg ctgctctttc taataatcta ctatgtaagt agaatacgtc acctgggtat
     3841 gcttcacgac ctggaggtct acgtaataat aatgataact cacgataagc tgctgcttgt
     3901 ttagttaaat catcataaac gattaaaaca tgtttaccgt taaacatgaa ttcttcaccc
     3961 attgttacac ctgaatatgg tgcaatatat aataatggag aaggttcaga agctgatgct
     4021 gctacaacaa tagtgtagtc taaagcgcct gcttgtctta acttttcaac atttgctctt
     4081 actgttgaat ctttttgacc aatagcaaca tagatacaaa tcgtaccttg atctttttgg
     4141 ttcaaaattg tgtcaattgc aattgttgtt ttacctgttt gacggtcacc aatgattaac
     4201 tcacgttgac ctctaccaat tggtactaaa gcatcaattg ctttgatacc tgtttgtaat
     4261 ggctcatcta ctgatttacg atccattaca ccagtagctt ttttctctac tggacgtgtt
     4321 ttagttgtgt taatcggtcc ttgtccatca ataggttgtc ctaatggatt aacaactctt
     4381 ccgattagtt cttcacctac tggtacttcc atgatacgac cagtacgttt aacttcgtca
     4441 ccttcagtaa tacctgtata tggtcctaaa ataaccacac ccacgtttga ctcttcaagg
     4501 ttttgggcta aaccaagtac gccgttatgg aattctacta gctcaccagc cataacgtca
     4561 tttaatccgt gaattaatgc aataccatca ccaatttgta atacagtacc tacatcagtt
     4621 acggacattt ctgactcata attttcaatt tgtgagcgaa gtaatgcact gatttcttca
     4681 gcttttatgg ccatctatgt cactcctctt tataattaat taactcttct aaattttctt
     4741 tgtaattgga caagatcatt tctaacacta ccatctaata cagttgtgcc gactttaact
     4801 ctaaatccac caattaaatc tggattaatt tttgtatcta caataacttt agataacttc
     4861 gtttgttgag ttactagttt gacaatctta tctaactctt cttgactcaa ttcatatgtt
     4921 gactcaattg ttgcaaaatc ttgattgtag tgtccgttat ataagctttg gaacgccttg
     4981 aatacgtcag ctattaatga gatatgtcta ttatctgcta acacgtacat catattttta
     5041 atgtatggat taatgtcagt aaatacacca ttaatcaact cacgacgttg ttctgctgtt
     5101 tgagttggat tactgtcaac cattttcaat tgctcaattt tatcttttac tgcttcattt
     5161 ataactgtta attcttcatt aatagtctct aaattatttg tatctaatga cacgtcaaat
     5221 aatgctttag catacttgtt agctactttt accattattt atcgcctgcc tcttttagat
     5281 acttgtcaac caatgctttt tggtcttgtt cagaaatttc ttttctaaga actttagaag
     5341 caattaacac tgatagttca gatacttgat tattaatatc tgcaatggca cgttcttttt
     5401 ggctattgat ttcactttgt gctgtttcaa tcataccgtt tgcacgtacg tttgcttcat
     5461 gaataatttg ttcttgctgt tgacgtgctt gaaccttagc atcttctaaa atcttttgaa
     5521 cttcttcttg tgtttcttta agtttttgtt tattttcttc ttcaagtttc tgtgcattta
     5581 acttagcttg ttctgcgtca tcgatatctc tgttaatatc tctttcacgt ttatccatta
     5641 catctttcaa tggaccccat gcgaactttt taagtaacgc aagtaacacg atgaaagtta
     5701 ggacctgtac aatcacagta ccccactcaa cgcctccagc tgcaccaaga acgaataagt
     5761 tagctgtttc agtcacgggt tcatgacact cctttcatta aaataatcgc tagtgttata
     5821 tagaaaaaaa cgacgaggtt aggacctact gaatttatgc gataggcatg gtgcattcat
     5881 atctcaacta tttaataaag ttcaaagata ttactatata tgcatcatga attatgtcct
     5941 atgcgctttg tcccgacctc ttttatctgt taattatcca gcaaatgtca tgaatgcaat
     6001 tactacaccg atgataggta atgcctcaac taaacctaca ccaatgaaca tgatacccat
     6061 taattgacca cgtgcttctg gttgacgtgc tacaccttca actgttcttg aaacgattaa
     6121 accgttaccg atacctgctc ctaatgctga taaaccaatt gcgattgctg ctgcgattaa
     6181 attcataatt taatttcctc ctgtgattgt atataattat tgaaattttt agtgttcatc
     6241 tgccacttta tgtgacatat aaaccatcga aagcataata aagatatatg cttggattgt
     6301 tcctacaaat attgaaaatg cttgccaaac aattaatcct gggatactaa taatccaacc
     6361 ccatgctggt tcgttaaaga ataagccagc aagtaatgtt aatagtatct cacctgcaaa
     6421 tatgttaccg tacaaacgca gaccaagcgt taatgttgaa gtgaactctt caaaaacatt
     6481 aataattgcc aatggccaaa acggctgtac ataaccttta agatattgtt tcgtaccacg
     6541 cattttaatt ccataaaagt gagttaacag tattatcgtt gtagacaacg ttaaagtcac
     6601 tgttgcatca gctgtcggtg atttccacca caatgtgtga tctttcgtta ctatagaaaa
     6661 cggaagacct aacatattag ctataaaaat gtacagaatc agcgttactg ctaagaagtg
     6721 gaattgacca ccttttttcc aagccatgtt accttcaatg attcccctca cgaaatcaaa
     6781 aatccattca acgaaatttt gtttgccagt tggtcttttt ttcaaattac gcgtacaaat
     6841 gatagcaagt agaaaaacaa gaaacgccgt aacaagtatc atcaatatac ttgataaatt
     6901 gaaaacgata tcaaaaccga ataaattcca actcacgagc ggggatttgt gatccataat
     6961 ctcacctctt tcttaattta tttgctcacc tttagtaaag gacgtatgat aactacaaca
     7021 tatgaaatca ttaaaccaat aattataccg atgatactta catgcgcttt attaaggtac
     7081 caaatcatac aggcaataat tgcaactaag tatcgccaca tatttccagt tgaaatatgc
     7141 atagtgtctg gtcttttagc tttagctaaa tagcattcaa aaatacatgt gttgattgca
     7201 gaaccagtaa caccaatgat taatcctaag ataaatgcat ggtgtgtata aaggtataat
     7261 cctcccaata ttattattag ataaaaataa tattgaatga actgtttaaa aatgttgtag
     7321 aaacgactca ttttatatgc ctccgtgtga ctgatatcaa tcatgaaaac cgttccactc
     7381 atacaaacta aatgataata taggcgattt gtggtgtcaa ttctattcaa attgttgtaa
     7441 aaaaataagc atagttagac acaatttaga cacaattaga caaaaagtct attttcctca
     7501 gaaatcgctt ggtttttcag tgattaaatt caaataatac ttaatatgat taactattct
     7561 ttctgaagca aatccatcac cgtaaggatt agatgcttca gacatttgat gatataatct
     7621 ctcatcatcg attaattctt ttgcagcttg atacacattt tgcttatttg ttccaatgac
     7681 ttttaatgtg ccagcttcaa caccttcagg acgttctgta acgcttcgca aaactaaaac
     7741 tggcttatta aatgatggcg cttcttcctg aattccacct gaatctgtca aaataaaata
     7801 agatttttta gcaaaattat ggaaatctac tacgtccaaa ggttcaatca attcaattct
     7861 gtcatgacta cctaaaatct tttgagccac ctctcgaact ttcgggtttt tatgcattgg
     7921 atataccagt gctaaatcag tatactcatc tattaagcgt ctaaccgctt taaatatatt
     7981 ttccatgggt ttcccgatat tttctcgtcg gtgtgctgtc ataagaatga attttttgtc
     8041 atggtattta tccatgatgt tagatttata attgtcatca actgtatatt tcatagcatc
     8101 aatcgcagta ttaccagtga caacaacact ttctgaatat ttcccttcac ttaacaaatg
     8161 cgatgcagca tttttagtag gtgcaaaatg taagtcagct aatacaccaa ctaattgtct
     8221 attcacctct tctggaaaag gtgaatattt atcataactt ctaagccctg cttcaacgtg
     8281 tccaatcggc acttggttat aaaatgccgc taaaccacct gcaaatgtcg tcatcgtatc
     8341 accatgtaca agtaccatgt ctggtttttc taattgaatc acttgttcta attgagtgat
     8401 tgatttagaa gttatctcag aaagtgtctg tcctgatttc ataatattca aatcgtattt
     8461 tggtttgatt tcaaaggtac ttaatactga atcaagcatt tctctatgct gtgctgtaac
     8521 aacaacaatt ggctcgagca ttttttcttg ttccaaagct ttaactaaag gagccatctt
     8581 tatggcttcg ggtcttgttc caaatatggt cataatcttt ttcatcaaac tacttatctc
     8641 cgattcttct atttagtacc aaacaatcta tctccagcgt cgcctaaccc tggtgtgata
     8701 tatgctttat catttagctt ttcatcaagt gcagcaatat aaatatctac atccggatgt
     8761 gcttcatgca tcttttctac gccttctggt gctgcaatta aacacatgaa gcgaatattt
     8821 ttagcgccac gtttcttcaa tgaagtaata gcttcaattg ctgatgcgcc tgttgctaac
     8881 ataggatcaa caacaatgat ttgtctttca gtaatatctt gaggtaactt agcaaaatac
     8941 tctacagcct ttaatgtttc gggatctcga tataaaccga tatgtccaac tctggctgca
     9001 ggtactaaac ttaaaatacc atcagtcata cctaaaccag ctcttaaaat tggaacgata
     9061 gctaattttt taccagctaa tcgtttagcc gtcattttag ttacaggcgt ttcaatatca
     9121 acatcctgaa gctctaagtc tctagttact tcatatgcca tcaacatacc aacttcgtct
     9181 acaagttctc taaattcttt agtacctgta tttacatctc taatatagct tagtttgtgt
     9241 tgaattaatg gatgatcgaa aacgtgtact ttactcataa aaattactcc tatctttgtg
     9301 tatgtttatt gatatagagg atattcagct gttaatttcg caacgcgttc tttagcttgt
     9361 tgtaattttt cttcatcttt actatttttc aatgctaaac tgatgatttt tgcaacttcc
     9421 tcaaaagctt tttcatcaaa tccacgcgtt gttgcagcag gtgtacctat acgtatacca
     9481 ctcgttacaa aaggtttttc ttgatcgaac ggaatggtat ttttgttaca tgtgatacca
     9541 actgaatcta aagtctcttc agcttcttta ccagtaagtc ctatagaccc ttttacatca
     9601 acagctacta agtgattatc tgtaccgcca gaaacaattc taaatccttc attaattaat
     9661 gcttctgcaa gaacttttgc gtttttaacc acttgttgtt gatacgtttt gaaattattt
     9721 tctaacgctt ctccaaaagc aactgctttt gctgcaataa catgctcaag aggtccacct
     9781 tgaataccag ggaaaattgt tttatctatg tcttttttat attcttcctt acataaaatc
     9841 ataccaccac gtggtccgcg taatgttttg tgtgttgtag ttgttacaaa atcagcatat
     9901 tctactggat ttggatgtaa acctgccgct actaatcctg caatatgtgc catgtctacc
     9961 attaacttag cgtttacttc atctgcgatt tctttaaact ttttgaagtc aattgttctt
    10021 gaatatgctg atgctcctgc cacaataagc ttaggcttat gctctaacgc taatttacga
    10081 acttcatcat aattgattcg ttctgtgtct ttatctactc catattcaac gaaattgtag
    10141 aatttaccac taaaattaac aggcgctcca tgtgtcaagt gaccaccatg actcaaattc
    10201 atacctaaaa ctgtgtcgcc catttctaat gcaactaagt aaacagccat gttcgcttgt
    10261 gaacctgaat gtggttgaac attgacatgt tcagctccaa acaatgcttt agcacgatca
    10321 attgcgatgc tttcagtaac atctacaaac tcacatccac cataatatcg gcgtcctgga
    10381 tagccttcag catacttatt agtcaacact gaaccttgtg cttccataac cgcttccgat
    10441 acaaaatttt ccgatgcgat taactctatg ttgctatttt gtctctgaaa ttctctctcg
    10501 attgcttctg cgataacttt atcttgcttg gtgatataag acataaaatc tccccttctt
    10561 tcaaaaaaac ttatttgtat ttagcacgtt cgccaccaat ctttttcggc ctagatgtgg
    10621 caatagttac aattgcctgt cctacttgct ttactgaggt ccttacaggt acacatacat
    10681 gtttaatatg catgcctatt aatgtttgac caatatcaat tccacaagga acagtaatat
    10741 gttcgaccac gatcggatcc ttcatatgct gaaaagcgta tgttgccaaa ctccctccag
    10801 catgtacatc tggaacgacg gaaacttctt ccattgttaa tggattatac tgagattttt
    10861 ctattgttat cgctctgttg atatgttcac atccttgaaa agcaaaagta acgcctgtct
    10921 ctttactcac aacatctaat gcattaaaaa tagtttctgc aacttccatc gaaccgacag
    10981 tccctatttt ttcgccaatg acttccgatg ttgaacatcc aattaaacat atatctcctt
    11041 tattaaaaaa ggacatttct tttaattcgt ctaataacat tgtcaaatct ttcataaaag
    11101 cccacccttc ctaaaaataa aaaaggaata tagcaaagtg ctacactcct ctattataac
    11161 ttatttaact gttaacatat actaattata cagaattcct actagcaaat aatatctttt
    11221 aattttaaaa ttaaacttac aagttcttca taggtatgta catacatttc ttttgttcca
    11281 ccgtatggat ctataacttc tcctgcttct tttacatatt catgcaatgt gaaaacatga
    11341 ttttgcaaac caaagtgtgc ctctattaat tctttgtgcg aatacgacat cgtcaaaata
    11401 atatctgctt tcaaatctgc ttcagtaaat tgttgcgata aggtcgtttc agctaaatga
    11461 tgttcttcaa ctaagtcttc aacataattc gaaacacctt gattgttcac agcgaatata
    11521 cctcttgatt caaattgatg atttggcata acctcttttg caatactttc cgctaatggg
    11581 ctacgacatg tgttacctgt acaaacgaat aaaatcttca tagttcacat cctttaataa
    11641 tgtgattacc tgcagctttt aacatgcgat tcataattgc ttctgtatta tcattcagct
    11701 caaagccgta tatatacgcc gctgaaatat tttcattttc atcaagtgaa tgtaacacat
    11761 cataaagatt atgacttgct tgtttaacat cattgtcatc ctgacataat tgaatgaatt
    11821 gcgcttcact tggtataaac gccaccttat tactcggcac aataaaagct atagaagacc
    11881 aatctttacc gtcatttcca attttgctct caatatccgt aataattgta agtggtgtat
    11941 tgggtgagta atgcttatac ttcatacctg gtgcaattgg ctgttcagta tcattataat
    12001 cagcatgggc gatactattc ggaagtattt ctgtaatcat tgctgctgtt atagaaccag
    12061 gtcttgcaat tttataagga aaagatgtgc aatctaaaac cgtactttct aatccttctt
    12121 cactttgttc agcttgaaca ataccatcta tacgggcatt caaatcttga tatacatgat
    12181 tgaaagttgt tggtgaaggt ctaccactta aattagcact tggagcagct agaggttcat
    12241 ttatgatttg taataattgt ctacctacag aatggcttgg cattctaaca gcaactgatg
    12301 ataaacctcc agaaactttt cgacatagat agcctggctt tagcggcaat ataaacgaaa
    12361 tagggcccgg ccagaatgcc tgcattaact tttctacgcg tggatccaaa gtatatgtaa
    12421 aatcttttaa ttgaacttta ctgtgtatat gaacaataag cggattgtca gatggacggc
    12481 ctttagcttc atatatttta gctacagctt cttcatctgt cgcatttgct gcaagtccat
    12541 aaactgtttc agttggtaaa cctattaaac caccgtttaa aacaatgtct tttatttcat
    12601 taattttagg atattgctgt aaatcttcat tatattctct aacatcccaa attttagtat
    12661 ccaacttaat cacgcctttc ttatttatca taatataaag caaaaagcta tgcacttaac
    12721 taatcatagc aaaggcataa cttctaatta ccatttaaat gagacgattc gatcgtggcc
    12781 atttatatct ttaataatgt cgattttttt gtcaggaaat ttatttaaaa ttattgattt
    12841 aagtgcctca ccttgattgt aaccaatttc aaaaacaact gggctgcctt tttccataac
    12901 gtgaggtaaa tctccaatga ttgattcata aatagcatat ccatggttat ctgcaaacaa
    12961 tgcctgatgt ggttcgaatc tcgtaaccgt tggagacatc gtaaccatat ctttttcatc
    13021 tatatatggt ggattagata tcaagccgtt caacttgata ccttctttaa ttaagggctt
    13081 taatgcatcc cctgttaaaa attgtatttg tgattgatgc ttctcagcat tattacgagc
    13141 catattcatt gcttcaagtg aaatatcagt agcaataaca tttaaatccg gcttttcaca
    13201 tttcaaagta attgcaagta caccactacc cgttccgata tctacgattg ttgcatcatc
    13261 ttctaactgt tgtaagaaat gcaacattac ttcttcagtt tcaggtcttg gtatcaaaca
    13321 atttgagttt acatcaaacg ttctaccata aaatgaggca aagccaacta tatactgtat
    13381 aggctctcct aataacatac gttgtaatgc taagtcgaac ttcataatca tcgctttcgg
    13441 catatcatca tgcatgtgaa ctacaaagtc cgtacgcgtc cattgaaata catctaacat
    13501 taaccattca gctcgtgttt gttcaaaccc tttttgttgt gttaaatgaa ttgcttcatc
    13561 taacttttct ttataattca ccattattaa gttctttcaa tttatctgtc tgctctgata
    13621 aagtcagtgc atctataatt tcttctaaat ggccttccat aatttgccct aatttttgaa
    13681 gcgttagacc tatacgatgg tctgttacac ggctttgtgg ataattataa gttcgaatac
    13741 gttctgaacg atcaccagta ccgactgctg atttacgttg tgacgcatac ttttgttgtt
    13801 cttcttgaac tttcatatcg tataaacgtg cttttaacac tttcattgct ttttcacggt
    13861 tttgaatttg agacttctca gaagatgttg caatgacacc agttggtaaa tgggtaatac
    13921 gtactgcaga gtcagttgtg tttacgtgct gaccacctgc accacttgaa cgatacgtgt
    13981 cgatttttaa atcttcattt ctaatttcaa tttctacatc ttcaacttct ggtaaaactg
    14041 ccactgtagc tgttgaagta tgaatacgtc cacctgattc tgtttcaggc acacgttgaa
    14101 cgcggtgcgc accattttca aatttcaatt tactatacgc gccattacca gaaactgaga
    14161 aactaatttc tttgtaacca ccatggtcac tttcagacgc ttctactatt tcagttttga
    14221 atccttgtga ttcagcatac tttgaataca tacgcattaa atcaccagca aaaatcgcag
    14281 cctcatcacc acctgctgct gctcttattt ctacaataac gtctttgtca tcattaggat
    14341 ctttaggaat caataatatt ttaagctctt cttcaagatt tggaagttca gctttaatac
    14401 cattactctc ctcttttaac atttctactt cttctttatc atcagtctca cttaacattt
    14461 cttcaatatc agctaattct tcttttttag ctttatagtt acgataaaca tctacagttt
    14521 tttgtaaatc agcttgctct ttagaatatt tacgtaattt atctgaatca tttacaacat
    14581 ctgggtcact taacagttca tttaactgtt cgtatctttc ttctacaata tctaattgat
    14641 caaacactta taattcctcc ttattattat cactaggtgc tacgatatgg tgcgcgcgac
    14701 aacgtggctc ataactttca ttggcaccta ctaagataat cggatcatcg attttagctg
    14761 gtttaccatt tattaatcgt tgcgttctac tagatgaaga accacaaaca gcacaaactg
    14821 cttgaagttt cgttacttgt tcactgacag ccatcaattt aggcattggt tcgaacggtt
    14881 cgcccctaaa atccatatct aatccagcaa caataacacg gtgtccatct gctgatagtt
    14941 tttctactat acttacaatt tcatcgtcaa aaaattgcac ttcgtctatt cctataacat
    15001 caacattagt taagttgtgc gtcataattt cacttgcttt agaaatatta atcgcttcaa
    15061 tggcattacc attatgagag accacttttt ctttatgata tcgatcatca atcgccggtt
    15121 taaatacaac gactttttgt ttagcgtata taccccttct tagacgtctt attagttctt
    15181 cggatttacc gctaaacata ctacctgtaa tacattctat ccaaccggaa tggtaagttt
    15241 catacattga gagttccacc tttttcaaaa cataatcgct ttattatatc atatttcaaa
    15301 tattcataaa tgtctttttc ataattatat cgatattgta catgaacaat tattttataa
    15361 atttgtcttg catatttata tacagctatt ctaatttgtt gcttcttatt cgtgcggtgt
    15421 ttctactcaa tacaaacttt aataaagggg gaaatttatc attatgtatt aacaacatgg
    15481 tattgagtca cttctttgaa cacagtcgta agtatatacc cttactacgc ttataaataa
    15541 aaaagctgtc tacaattcca ttataggatg cagacagctt aacgattact atattagttg
    15601 tttgatttaa gaccaaactt tttgttgaaa cgttccacac gtccatccgc agcagcgaat
    15661 ttttgacgtc cagtgtagaa tggatgtgaa tcagatgaaa tatctaaacg aataactggg
    15721 tattcttttc catcttccca ctccatcatt tctgaagatg tttttgtaga accgcttaag
    15781 aatttgaagt ttgtagtagt atctaaaaag attacttggt gatactcagg atgaattcct
    15841 tgtttcatta ttttcagctc ctttgccctg aaccatctgg aacagagtta tttgtgagtt
    15901 tttacccaat actgtgtaat tataaaggta attaattcaa aacgcaagcc cctatataat
    15961 gtttattaaa ttataggtcg acccgtttta gtactttctt ctgcagactt ttgtagctgc
    16021 ttgaagaaat cttcattatt cttagacctt ttaagtttgc gaataaatct ttcagtaaag
    16081 tcagttgagt cagtgaatag atttcttaat tgccataatg tgtctaattc agatttactt
    16141 attaacaatt cttctttacg cgttgaactt ctgccaatat caattgcagg gaagatacga
    16201 cgttcagaca atttacgatc taaatgtaac tccatgttac ctgttccttt aaattcttcg
    16261 taaatcatat cgtccatacg tgaacccgta tcaactaatg cagttgcaag tattgttaaa
    16321 cttccacccg cttcaatatt tctcgctgca ccgaagaatg cttttggttt gtgtaaagat
    16381 gcaggatcta aaccacctga taatgtacga ccacttggtg gaataactaa gttataagcg
    16441 cgtgctaatc tcgttataga atccattaaa ataatgacat cttccccaat ttctactaaa
    16501 cgctttgcac gttcaagtaa taattcagct actttaacat ggtgttctgg tggttcgtca
    16561 aacgttgaat gaacgacttc agcagcttct actgagcgtt ctaaatctgt cacctcttca
    16621 ggacgctcgc caactaacaa aataaatagc tttgcatctg gtttgttcgt actgattgca
    16681 ttcgctattt cttttaataa cgatgtttta cctgctttag gtggcgccac tattaaacca
    16741 cgttgaccta aaccaatcgg tgttactaaa tccatgatgc gcgttgaata attttgtgtt
    16801 tctgtctcta atttaatacg ctcatctgga taaagtggtg tcaaagcttg gaaatgcgga
    16861 cgtttcttca cttcttctgc gttatggtca ttgacaaagt caacttgtaa taagccataa
    16921 tatttttcgt tatctttagg ttttctaact ttcccagtta ctttatcccc acgtttaatt
    16981 tcaaaacgac gaatttggct agcagatata taaatatctt tttccccttt agaatagttc
    17041 actgttctta aaaaaccata accatctggt tgtatatcat ctaagatacc ttccatataa
    17101 tagttaccat ctttttccat ttgtgcttcc ataatagcta gaacaagttc ttttttattt
    17161 aatttactat agttcgtcaa cttaagagtt ttagcttttt gagtgagttc cttggtagta
    17221 tagttcttgt acaattcgtg gaatgattca tactgaggag atgtacgttc tctttcaggc
    17281 atatagttta cacccatttc attatttttt agttagtaag taatagatag gcatgacaat
    17341 gcataaataa ctgtgctagc acaatcccat tatgctaatc cccaaccttt taaatacatg
    17401 aataactata gcaaattttt attttaatta caaaatctat ttataaaaat tgcaaaatta
    17461 aaacagtaca caaaacttaa ttagtcatgt gcactgtttt attgcgattt ttaattcaaa
    17521 aatcaaattt atttaaaata tccagctata gatttcactt ctaagaactc ttcaatacca
    17581 taatcgcccc attcacgacc taaaccagat tgtttatagc caccaaatgg taaatctggc
    17641 tttctacctg cttcgtttat ttctactgta cctgcttcaa tagaacgagc tactttatgc
    17701 aatgtttctt tgtccttacc aataacatat cctgccaaac catattttgt atcatttgca
    17761 atttgaatcg cttcatctaa atcgttataa gtgataactg acattactgg cccaaaaatt
    17821 tcttcttgtg ctatcgtcat ttgattatct acattaataa aaattgtcgg acgtgcaaag
    17881 tatccttttt caagtccttc tggtttacca ggaccaccat aaaataattc agcaccttct
    17941 tcaatacctt tattaatata attttgtact tgatcaaatt gttttttact aatgataggg
    18001 cctacttgtg taccatcttc tcttggatta ccgacacgca cttggctaaa ttgttctttt
    18061 aattcagcta agaatgcatc tttaattttg ttaggcacta aaacacgtgt accagctgta
    18121 catacttgac cagtattatt aacaactttg cctgttgttg ctttagccgc ttctttaata
    18181 tctacgtcat ctaggacgat atatggtgat ttgccaccaa gctctaatga taccttttta
    18241 aaatctttag cggctttttc cataatttta gaaccagtag ggcctgatcc tgtgaatgac
    18301 atcatgcgta ctttaggatg ttcagataaa ggattcccaa caccagcacc atcaccatta
    18361 acaaggttaa atacaccttt aggaacaccg actttatcaa aaatctcagc taaaataaca
    18421 gctgcaaatg gtgtttcttc agatggttta agtacaactg gactaccagc cgcaaatgct
    18481 gctgctaatt ttaatgatgt ttggtttgta gggaagttcc acggtgtaat taatccagat
    18541 acaccgattg cttctttaac aactaaatca tctccgcggc gttcttcaaa ttcgtagtta
    18601 tctaatgcgt ctctcgctgc aacaaaatgg tttagtccca tttgataatg gacacgctca
    18661 gataatgata aaggagcacc taattcatcc gtaatagctt gtacaatatc gtcttttctg
    18721 ttttcatatt cttttacaat tttatctaat aacgcttgtc tttctttcac agatgtatga
    18781 cggaactcta aataaacatc gtctgccgcc tcgacggctt tatcaacatc agctttatta
    18841 cctttagcaa ctttcccgat tacttcttcg gttgctggat ttataacttc tatcgtttca
    18901 ttactattac tttctaccca ttcgccatta atgtattgct ttgtgtagtc tctcattatt
    18961 ctttcactcc tcaagaatga tttatatatt atttatccgt aatgcatacg atataaacat
    19021 tacattccat tatgatacgc tccattttaa tatgttcaaa ataaaatgct t
//
LOCUS       DANBWH010000027        18683 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.027, whole genome shotgun sequence.
ACCESSION   DANBWH010000027 DANBWH010000000
VERSION     DANBWH010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18683
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.027"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..327)
                     /locus_tag="PPY92_000648"
     CDS             complement(<1..327)
                     /locus_tag="PPY92_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624671.1"
                     /translation="MKQTFITLGEGLTDLFEFMTMIEYNHQRIDKIIYFHSPQAENKK
                     SSVAIIMNPTTGNHFQAFYIMINAIKYPYPDSNKKFQMINDCAEKFDIPILGIDVQPP
                     QAFHDLS"
     gene            397..939
                     /gene="rsmD"
                     /locus_tag="PPY92_000649"
     CDS             397..939
                     /gene="rsmD"
                     /locus_tag="PPY92_000649"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDJ2624672.1"
                     /translation="MRVIAGKHKSKALESMEGRNTRPTMDKVKEGIFNSLYDVSGIGL
                     DLFAGSGALGIEALSRGMDKVIFVDQNFKAVKVIKSNLANLDLEAQSEVYKNNADRAL
                     KALSKRDIQFDVIFLDPPYNKGLIDKALKLISEFNLLKENGIIVCEFSNHEEIDYQPF
                     NMIKRYHYGLTDTLLLEKGE"
     gene            941..1423
                     /gene="coaD"
                     /locus_tag="PPY92_000650"
     CDS             941..1423
                     /gene="coaD"
                     /locus_tag="PPY92_000650"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDJ2624673.1"
                     /translation="MEHTIAVIPGSFDPITYGHLDIIERSTDRFDEIHVCVLKNSKKE
                     GTFSLEERMDLIEQSVKHLPNVKVHQFSGLLVDYCEQVGAKTIIRGLRAVSDFEYELR
                     LTSMNKKLNNEIETLYMMSSTNYSFISSSIVKEVAAYRADISEFVPPYVEKALKKKFK
                     "
     gene            complement(1485..2624)
                     /locus_tag="PPY92_000651"
     CDS             complement(1485..2624)
                     /locus_tag="PPY92_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDJ2624674.1"
                     /translation="MKSVGLITEYNPFHNGHQYHINQSKKLTNADVTIAIMSGNFVMR
                     GEPAIYNKFTRAKMALSTADLVIELPATASLSSGDHFAELAVKVADYMSVDTIAFGSE
                     NNDIKTLKQLAHSINEIEQSESFSQKVKEGKSYPRIISELLEHHEALASPNNILGISY
                     LKAIAKNAKNINAISIKRENAQHHDSLIQHHQFASGTSIRTSIISQDDHWHHVVPKDI
                     QHLYVTPHITLNQIFPYLKYQIIAMTTDSLKNIYTVTEGFENRLKSNIYEATDFHHFV
                     KLLKTKRYTYTHIQRLLMNVLLNIKPTDVTSNIHAVKVLAMNDRGRQYLKHLKTAFPE
                     RQYITNINKSNAHYFTNEIKATHIYNAISGQQQTDFNTPVIQQYR"
     gene            2751..3308
                     /locus_tag="PPY92_000652"
     CDS             2751..3308
                     /locus_tag="PPY92_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDJ2624675.1"
                     /translation="MKWSITQLRKYQGKPFEFDQTVSFDNLKESLDLIDLSPITIQGQ
                     LTIKSTEVVADIHITGTYTMPCARTLVPVKVPLDVTTTEVFDLEGYNQYNDDQDDVDE
                     HYHIIKDGMVNLQDIVEDIVIIEKPMRAYSEQSDQMLTVGNGWEVIDEDQLDELAKQQ
                     EQDDSESRQVDPRLQKLQQLYDKEQ"
     gene            3388..3561
                     /gene="rpmF"
                     /locus_tag="PPY92_000653"
     CDS             3388..3561
                     /gene="rpmF"
                     /locus_tag="PPY92_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDJ2624676.1"
                     /translation="MAVPKRRTSKTRKNKRRTHFKISVPGMTECPNCGEYKLSHRVCK
                     NCGSYNGEEVAAK"
     gene            complement(3678..5063)
                     /locus_tag="PPY92_000654"
     CDS             complement(3678..5063)
                     /locus_tag="PPY92_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001657254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDJ2624677.1"
                     /translation="MKVAIYTRVSTLEQKEKGHSIEEQERKLRAYSDINDWKIHKVYT
                     DAGYSGAKKDRPALQEMLNEIDNFDLVLVYKLDRLTRSVKDLLEILELFENKNVLFRS
                     ATEVYDTTSAMGRLFVTLVGAMAEWERTTIQERTAMGRRASARKGLAKTVPPFYYDRV
                     NDKFVPNEYKKVLRFAVEEAKKGTSLREITIKLNNSKYKAPLGKNWHRSVIGNALTSP
                     VARGHLVFGDIFVENTHEAIISEEEYEEIKLRISEKTNSTIVKHNAIFRSKLLCPNCN
                     QKLTLNTVKHTPKNKEVWYSKLYFCSNCKNTKNKNACNIDEGEVLKQFYNYLKQFDLT
                     SYKIENQPKEIEDVGIDIEKLRKERARCQTLFIEGMMDKDEAFPIISRIDKEIHEYEK
                     RKDNDKGKTFNYEKIKNFKYSLLNGWELMEDELKTEFIKMAIKNIHFEYVKGIKGKRQ
                     NSLKITGIEFY"
     gene            complement(5270..5950)
                     /locus_tag="PPY92_000655"
     CDS             complement(5270..5950)
                     /locus_tag="PPY92_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDJ2624678.1"
                     /translation="MEENAPLETAVNNFKKIQNSEIYKFKYMNSWCLEYSEFLLDEVR
                     LLKENKSYTRYKKGTIIYVKLGVNVGREFSGNHFCMVLNNHDSNKNPILTVVPLTSSR
                     SKFNVHIEEDLLPLVLEKMDVTGKDLAKKIMNNLEKVSKAENPYDQKLLDENKSLNDD
                     FKKYSKVRKRYERFKYKKTYANVLNITTISKDRISKINRYDPAGEISYSKETVDKIEN
                     SIKIRFLS"
     gene            complement(5982..6443)
                     /locus_tag="PPY92_000656"
     CDS             complement(5982..6443)
                     /locus_tag="PPY92_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015977706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDJ2624679.1"
                     /translation="MGKYEELLMKCEVEVKETQRVPRGFDGWYQEGEIFIRPSLSERN
                     KLEVLYEELAHHKLTYGNILDQSKFNNRKFENYARRHGFISAVPLREIVEAYNYGVRN
                     LYELSEYLQLSEEYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKLHHID"
     gene            complement(6465..6791)
                     /locus_tag="PPY92_000657"
     CDS             complement(6465..6791)
                     /locus_tag="PPY92_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDJ2624680.1"
                     /translation="MEYKSARKILSENLEQLMKENNITQVELSEAIGVSQSTISNWLK
                     ELKYPRISKVQQLADYFNVPKSRITEERSIHQETIAGHANKDEFTPEEWEEIENFMQW
                     VRDRKK"
     gene            6954..7163
                     /locus_tag="PPY92_000658"
     CDS             6954..7163
                     /locus_tag="PPY92_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDJ2624681.1"
                     /translation="MPEDFKEFSVKVWRTNSNMTQQDVADKLGVTKQSVIRWEKDSTE
                     LKGLQLYALAKLFNTEVDYIKAKKI"
     gene            7203..8084
                     /locus_tag="PPY92_000659"
     CDS             7203..8084
                     /locus_tag="PPY92_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDJ2624682.1"
                     /translation="MLIDINKVTVGKRIRKDYGDITSLADDIEDRGLINPPVVTPDYE
                     LIAGERRLKAMKKLDYRQIEVRVMSVEDYEHQLKIEISENEERKAFTYSERMDYAKQL
                     ERIEAKKAKDRKTSKLKQNKDTVTDQGPERKGETRDIVGKASGFGSGRTYARAKYIYE
                     NADEETIKEVDEGKKSIRKAHDELRAKEKQNEANKVKVTTKEKPQTTVVDRRERMNAE
                     VEAMSETETNLALSEAAAANIVNVCSNLLYAVNNIEDLELTLNFLKSRDAEELSKVVK
                     ASKALNKIIEKGDFINV"
     gene            8077..8844
                     /locus_tag="PPY92_000660"
     CDS             8077..8844
                     /locus_tag="PPY92_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDJ2624683.1"
                     /translation="MYNQQLPVHRLQTDMSYQSPVQERQVRKIVNKFDSKKLHTIVVS
                     KRKDGSFYIIDGQHRVEALKELNISFIEATVHENLTIEEEAEMYYGVNDRPSKNANSK
                     GKSRLRFKEPVAVAIDETVKSVGLEIDYEKSASTKGYIKAYDALQSIYKGNGANHLGL
                     VLEIIKDSFGEDTRNYQSFILRGFSKLLKVYLHEVDLNFLVKKLQNIGYEGFILEINK
                     KHAGFKTKKECLPFVVVDIYNKNRRQKNQLDKMKLHV"
     gene            8915..9094
                     /locus_tag="PPY92_000661"
     CDS             8915..9094
                     /locus_tag="PPY92_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624684.1"
                     /translation="MEQTIKQFLEFRKQFTPAQWHEINRIIDGQFSKKAAELQLDDQD
                     VEVIKNIITQQKIMK"
     gene            complement(9195..9677)
                     /locus_tag="PPY92_000662"
     CDS             complement(9195..9677)
                     /locus_tag="PPY92_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015977661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624685.1"
                     /translation="MEFIQSTLFSNVVAFLALGLSAYSIFYTRSQNKFSFVISDLNFY
                     YENNFVELNFVVANDSSRTHTLEELIFLDKNKNVLTPINVVLESDEYSSLGIYNPSYL
                     HAPIDKQLDKPEVMIANSSSEFLYKFELEPAFIKIVSNQRINKLKKYKLISTDSYEHN
                     "
     gene            9737..10504
                     /locus_tag="PPY92_000663"
     CDS             9737..10504
                     /locus_tag="PPY92_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HDJ2624686.1"
                     /translation="MQALQTFNFEELPVRTLTVNEEPYFVGKDVADILGYKNGSRDIN
                     AHVDAEDKLTYQISTAGQRRNQTIINESGLYSLIFSSKLESAKRFKRWVTSDVLPAIR
                     KHGIYATDNVIEQTLKDPDYIITVLTEYKKEKEQNLLLQQEIGELKPKADYVDEILKS
                     TGTLATTQIAADYGISAQKLNKLLHEARLQRKVNKQWVLYSEHMGKSYTDSDTITIVR
                     SDGREDTVLQTRWTQKGRLKIHEIMTEFGYEANLGGA"
     gene            10505..10729
                     /locus_tag="PPY92_000664"
     CDS             10505..10729
                     /locus_tag="PPY92_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624687.1"
                     /translation="MTPEQKEKLNNIVLTLYAVKENKSQTYTHKDTLTVTYAGEIEHT
                     YEVDREKHLESMIEWAIDQIEQHFDLDEEE"
     gene            10769..10912
                     /locus_tag="PPY92_000665"
     CDS             10769..10912
                     /locus_tag="PPY92_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624688.1"
                     /translation="MLQKFRIAKEKSKLKLNLLKHANSNLETRNNPELLRAVAELLKE
                     INR"
     gene            complement(10934..11581)
                     /locus_tag="PPY92_000666"
     CDS             complement(10934..11581)
                     /locus_tag="PPY92_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002478862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624689.1"
                     /translation="MNNINLTQRQLDLIKKNKAILSKLPVEAYAKATNTMNNSYVMNA
                     LEIQSTVNNVMNSIRINQSKLSDWASYMHRVTKNHPMFKTNLFSEKILDEFISSNNFP
                     EDEVRKVSTHLRNSFVDTVDVPVLGKTVDSAHPIDDVNTKKYNSVFNESLNHIFISPS
                     AKFIKKVSVGSAIGVTSPVMVRTILDQYVNYFMFFNVIATLLTLYVIANYLDDEN"
     gene            11761..11922
                     /locus_tag="PPY92_000667"
     CDS             11761..11922
                     /locus_tag="PPY92_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 family protein"
                     /protein_id="HDJ2624690.1"
                     /translation="MSNIYKSYLVAVLCFTVLAIVLMPLLYFTTAWSIAGFASIATFM
                     YYKECFFKE"
     gene            12014..12274
                     /locus_tag="PPY92_000668"
     CDS             12014..12274
                     /locus_tag="PPY92_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HDJ2624691.1"
                     /translation="MYYEIGEIIRKNIHVNGFDFKLFILKGHMGISIQVKDMNNVPIK
                     HAYVVDENDLDMASDLFNQAIDEWIEENTDEQDRLINLVMKW"
     gene            12284..12505
                     /locus_tag="PPY92_000669"
     CDS             12284..12505
                     /locus_tag="PPY92_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2483 family protein"
                     /protein_id="HDJ2624692.1"
                     /translation="MKQTVTYIIRHRDMPIYITNKPTDNNSDISYSTNRNRAREFNGM
                     EEASINMDYHKAIKKTVTETIEYEEVEHD"
     gene            12498..13286
                     /locus_tag="PPY92_000670"
     CDS             12498..13286
                     /locus_tag="PPY92_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001668918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDJ2624693.1"
                     /translation="MTEEKQEPQEKVSILKKLKINNIAEKNKRKFYKFAVYGKIGSGK
                     TTFATRDKDAFVIDINEGGTTVTDEGSDVEIENYQHFVYVVNFLPQILQEMRENGQEI
                     NVVVIETIQKLRDMTLNDVMKNKSKKPTFNDWGEVAERIVSMYRLIGKLQEEYKFHFV
                     ITGHEGINKDKDDEGSTINPTITIEAQEQIKKAITSQSDVLARAMIEEFDDNGEKKAR
                     YILNAEPSNTFETKIRHSPSITINNKKFANPSITDVVEAIRNGN"
     gene            13315..13866
                     /locus_tag="PPY92_000671"
     CDS             13315..13866
                     /locus_tag="PPY92_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDJ2624694.1"
                     /translation="MKITGQAQFTKETNQEKFYNGSAGFQAGEFTVKVKNIEFNDREN
                     RYFTIVFENDEGKQYKHNQFVPPYKYDFQEKQLIELVTRLGIKLNLPSLDFDTNDLIG
                     KFCHLVLKWKFNEDEGKYFTDFSFIKPYKKGDDVVNKPIPKTDKQKAEENNGAQQQTS
                     MSQQSNPFESSGQFGYDDQDLAF"
     gene            13879..14550
                     /locus_tag="PPY92_000672"
     CDS             13879..14550
                     /locus_tag="PPY92_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624695.1"
                     /translation="MQYITRYQKDNDGTYSVVATGVELEQSHIDLLENGYPLKAEVEV
                     PDNKKLSIEQRKKIFAMCRDIELHWGEPVESTRKLLQTELEIMKGYEEISLRDCSMKV
                     ARELIELIIAFMFHHQIPMSVETSKLLSEDKALLYWATINRNCVICGKPHADLAHYEA
                     VGRGMNRNKMNHYDKHVLALCREHHNEQHAIGVKSFDDKYHLHDSWIKVDERLNKMLK
                     GEKKE"
     gene            complement(14656..15513)
                     /locus_tag="PPY92_000673"
     CDS             complement(14656..15513)
                     /locus_tag="PPY92_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4393 domain-containing protein"
                     /protein_id="HDJ2624696.1"
                     /translation="MDPILGKGIDKIIEGASKGPVETFSKTWELVFGKFHLYVDKVIY
                     QREVEFEKFKEQFKKEISSVPENNLQEPQFSLLGPALEASKFYISEKTLSNMFAKLIA
                     SSMDDRKNSLTHHSFVEIIKQLSPNDAILLKHLKNHEVHPAVKYRAVLNPKNDGMNIS
                     DTLIKDSPLDIESTEISINNLVRLGVLNETFDMSYLTKKGIYNKFYAPQFLNHFNKII
                     EKQRFVSGLEFVKRMLKSGHNLETISKLSGIEFEVLKLHYSPWVIDIKKGSISLSAYG
                     KAFVKTCIN"
     gene            15577..16314
                     /locus_tag="PPY92_000674"
     CDS             15577..16314
                     /locus_tag="PPY92_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001668920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDJ2624697.1"
                     /translation="MTDQPSYYSIITANVRYDNRLTDSEKLLFAEITSLSNKYGYCTA
                     SNGYFAKLYEVTKVTVSRRIANLKECGYLHVEIIRNGNEIKQRKLYPLTEMIRPINTN
                     DNTPINNSVNTPIITNVKENNTSINNTSNNNINRVDILSGNPTRIPYKEIIDYLNEKT
                     GKKFKHNTTKTKDFIKARWNQDFRLEDFKKVIDIKTAEWLNTDSDKYLRPETLFGNKF
                     EGYLNQKVQPTGTDQLERMKYDESYWD"
     gene            16324..17103
                     /locus_tag="PPY92_000675"
     CDS             16324..17103
                     /locus_tag="PPY92_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDJ2624698.1"
                     /translation="MKQLFSEKINESLKKYQPTHVEKGLKCERCGSEYDLYKFAPTKK
                     HPNGYEYKDGCKCEIYEEYKRNKQRKINNIFNQSNVNPSLRDATVNNYKPQNEKQVHA
                     KQTAIEYVQGFSTKEPKSLILQGSYGTGKSHLAYAIAKAVKAKGHTVAFMHIPMLMDR
                     IKATYNKNAVETTDELVRLLSDIDLLVLDDMGVENTEHTLNKLFSIVDNRVGKNNIFT
                     TNFSDKELNQNMNWQRINSRMKHNARKVRVIGDDFRERDAW"
     gene            17097..17255
                     /locus_tag="PPY92_000676"
     CDS             17097..17255
                     /locus_tag="PPY92_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624699.1"
                     /translation="MVTKEFLKIKLECSDMYAQKLIDEAQGDENKLYDLFIQKLAERH
                     TRPAIVEY"
     gene            17268..17489
                     /locus_tag="PPY92_000677"
     CDS             17268..17489
                     /locus_tag="PPY92_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3269 family protein"
                     /protein_id="HDJ2624700.1"
                     /translation="MPKEKYYLYREDGTEDIKVIKYKENENEVYSLTGAHFSDEKKIM
                     TDSDLKRFKGAHGLLYEQELGLQATIFDI"
     gene            17499..17903
                     /locus_tag="PPY92_000678"
     CDS             17499..17903
                     /locus_tag="PPY92_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HDJ2624701.1"
                     /translation="MSKYNAKKVEYKGIVFDSKVECEYYQYLESNMNGTNYDRIEIQP
                     KFELQPKFGKQRPITYIADFSLWKDGKLVEVLDVKGKATEVANIKAKIFRYQYRDVNL
                     TWICKAPKYTGQEWMVYEDLVKVRRKRKREMK"
     gene            17908..18093
                     /locus_tag="PPY92_000679"
     CDS             17908..18093
                     /locus_tag="PPY92_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3113 family protein"
                     /protein_id="HDJ2624702.1"
                     /translation="MQQQAYINATIDIRIPTEVEYHHFDDVDDEKDMLAKRLDDNPDE
                     LLKYDNITIRHAYIEVE"
     gene            18094..18465
                     /locus_tag="PPY92_000680"
     CDS             18094..18465
                     /locus_tag="PPY92_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624703.1"
                     /translation="MAKTARIVRIHDKPYRFSKFEMELIESHGITAGMVSKRVKDGWE
                     LHEAMDAPEGTRLSEYREKKTIERLEQARLERKLERKRKREAELRRKKPHLFNVPQKH
                     SRDPYWFDNTYNQMFKKWQEV"
     gene            18466..>18683
                     /locus_tag="PPY92_000681"
     CDS             18466..>18683
                     /locus_tag="PPY92_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624704.1"
                     /translation="MPKTDSACKEYLNQFFGSKRYLYQDNERVAHIHVVNGTYYFHGH
                     IVPGWQGVKKTFDTTEELETYIKQHGLE"
BASE COUNT         6797 a         2874 c         3321 g         5691 t
ORIGIN      
        1 cgataaatca tgaaatgctt gagggggctg tacatcgata cctaaaattg gtatgtcgaa
       61 tttttcagca caatcattaa tcatttgaaa ctttttattt gaatctggat atggatattt
      121 aatagcgtta atcatgatat aaaatgcttg gaaatgattg ccagttgtag ggttcataat
      181 gattgctaca gatgactttt tattttcagc ttgtggtgaa tgaaaataga taattttatc
      241 aatacgttga tggttatatt caatcatcgt catgaattcg aacaaatctg ttagaccttc
      301 accaagtgta ataaatgttt gtttcattgg tttatcattc tcctttttct caataaatta
      361 tattaaataa ctaaatgaat tggaagtgtg aacaacatgc gcgtcattgc aggtaaacat
      421 aaaagtaaag ctttagaaag tatggaaggc cgtaatacga gaccaactat ggataaagtt
      481 aaagaaggta tctttaatag tttatatgat gtgtcaggta taggtttaga tttatttgca
      541 ggaagcgggg cgcttggaat agaagcactc tctcgaggta tggataaggt aatctttgtt
      601 gatcaaaatt ttaaagctgt aaaagttatt aaatcaaatc ttgcgaattt ggatttagag
      661 gcacaatctg aagtttataa aaataatgca gatagagctt taaaagcatt gtcaaaacgt
      721 gatattcaat ttgatgtcat tttcttagat ccaccttata ataaaggtct cattgataaa
      781 gctttaaaac taatttcaga gtttaattta ttgaaagaaa atggtatcat cgtttgtgaa
      841 tttagcaatc atgaagaaat agactatcaa ccgtttaata tgattaaacg ttaccattat
      901 gggttgacag acacattgtt attagaaaag ggagaatagc atggaacata caatagcggt
      961 cattccgggt agttttgacc ccattactta tggtcattta gacattattg agagaagtac
     1021 agatagattt gatgaaattc atgtctgtgt tcttaaaaat agtaaaaaag aaggtacgtt
     1081 tagtttagaa gagcgtatgg atttaattga acaatctgtt aaacatttac ctaatgtcaa
     1141 ggttcatcaa tttagtggtt tactagtcga ttattgtgaa caagtaggag ctaaaacaat
     1201 catacgtggt ttaagagcag tcagtgattt tgaatatgaa ttacgcttaa cttcaatgaa
     1261 taaaaagttg aacaatgaaa ttgaaacgtt atatatgatg tctagtacta attattcatt
     1321 tataagttca agtattgtta aagaagttgc agcttatcga gcagatattt ctgaattcgt
     1381 tccaccttat gttgaaaagg cattgaagaa gaaatttaag taataaaaat aacagtattt
     1441 taggtttatc atggtttaca atcctaaaat actgttttca tttgttaacg atattgctgt
     1501 atgacaggcg tgttgaaatc tgtttgttgt tgcccgctta ttgcattgta tatgtgtgtt
     1561 gctttgattt catttgtgaa gtaatgtgca ttgcttttgt taatattggt tatatattgt
     1621 ctttctggga acgctgtttt taaatgcttt aaatattgtc tgccacggtc gttcatcgct
     1681 aatactttaa ctgcgtgaat gttactcgta acatctgtag gtttaatgtt taataataca
     1741 ttcattaaca gtctttggat atgcgtatat gtataacgct ttgtttttag taattttaca
     1801 aaatgatgaa aatcagttgc ttcataaatg ttagatttca aacgattttc aaaaccttca
     1861 gtaacagtat aaatattttt taatgaatct gtagtcatag ctatgatttg atatttcaaa
     1921 tatggaaata tttgatttaa tgttatatga ggtgttacgt acaagtgttg aatatcttta
     1981 ggtaccacat gatgccaatg atcatcttga ctaatgattg atgttctaat agatgtacca
     2041 cttgcaaact gatggtgttg aattaatgaa tcatgatgtt gagcattttc tcgtttgata
     2101 gaaattgcat tgatgttttt agcattttta gcaattgctt tcaggtaact aataccaagt
     2161 atgttgttag gacttgctag tgcttcatga tgctctaata attcgctaat gatacgaggg
     2221 tagcttttac cttcttttac tttttgtgaa aaggattcag attgttcaat ttcattaatg
     2281 ctgtgtgcta attgctttaa tgttttgata tcattatttt cactaccaaa tgcaattgta
     2341 tcgacactca tataatctgc gactttaact gctagttcgg caaaatgatc gccagatgat
     2401 aaactggcag ttgctggtag ttcgataact aaatcagctg ttgataatgc catttttgca
     2461 cgagtaaact tattatagat tgctggttcg ccacgcatga caaagttacc actcattatt
     2521 gcaatagtaa cgtcagcatt tgtaagtttt ttagattgat taatatgata ttgatgccca
     2581 ttatgaaagg gattatattc tgtgattaag ccaacgcttt tcatttgcaa ttcgtccttt
     2641 cggtattcat tattaatatt gtaacagaac atgatatgtt aagaaaaaat cttgacaact
     2701 tgttcttaga aagttaaaat aaattttgtg cttgttagag gtgaagccat atgaaatggt
     2761 caattacgca attaaggaaa tatcaaggta agccatttga atttgatcaa acggtgagtt
     2821 ttgacaattt aaaagaatca ttagatttaa ttgatttatc tccaattaca atccaaggtc
     2881 agttaaccat taagtcaaca gaagtcgttg cggatattca cattactgga acgtatacaa
     2941 tgccttgtgc acgtactctt gtaccagtaa aagtcccact agatgtaact actacagaag
     3001 tatttgattt agaagggtac aatcagtata acgatgatca agatgatgta gatgaacact
     3061 atcacattat taaagatggt atggttaatc ttcaggatat tgtcgaggat atagttatta
     3121 ttgagaaacc aatgagagct tattcagagc aaagtgacca aatgttgaca gtaggtaatg
     3181 gttgggaagt aatcgatgag gatcaattag atgagcttgc taaacagcaa gaacaagatg
     3241 attcagaatc acgacaagtt gatccaaggc ttcaaaaatt acaacaatta tatgataaag
     3301 agcaataagt agtttaatta atgatataat gttgttaatt aaactaacat ttagtatttt
     3361 tcgtttataa tctaaggagg atatatcatg gcagtaccaa aaagaagaac ttctaaaact
     3421 agaaaaaaca aacgtcgtac gcatttcaaa atttcagtac caggtatgac tgaatgccca
     3481 aactgtggcg aatacaaatt atcacaccgt gtatgtaaaa actgtggttc ttacaatggc
     3541 gaagaagtag cagctaaata attttagtta ctcaatataa aaagtcccgc ttaaaatgat
     3601 tgttttaagt gggacttttt atattgcgaa aaataattgg cgaacgaggt aactgcatag
     3661 ttattccgaa cttccaatta ataaaactct atacccgtaa tcttcaatga gttctggcgc
     3721 ttccctttaa ttccttttac atattcaaaa tgaatgtttt tgattgccat ctttatgaat
     3781 tcagttttta actcatcttc cattaattcc cagccgttta gcaatgaata cttgaaattt
     3841 ttaatcttct catagttaaa agtcttaccc ttatcattat ccttgcgctt ttcatactca
     3901 tgtatttctt tgtcaatacg acttattatt ggaaaagctt catccttatc catcatacct
     3961 tctataaaaa gtgtttgaca tctagcgcgt tcttttcgca acttttcaat atcgatgccg
     4021 acatcttcta tttctttagg ttggttttcg attttatatg atgttaaatc aaattgtttt
     4081 agataattgt aaaattgttt taaaacctcg ccttcgtcga tgttacatgc atttttattt
     4141 ttagtatttt tgcagttaga acaaaagtat agtttagaat accaaacttc tttattttta
     4201 ggcgtatgct tgactgtgtt taaagtcaat ttctggttac agtttggaca taatagttta
     4261 cttctgaaaa tagcgttatg ttttacgatt gtagagttag ttttttcact tatccttaat
     4321 tttatttctt cgtattcttc ttcacttata atagcttcgt gggtgttttc gacgaatatg
     4381 tcaccgaaaa caagatgacc tctagctacc ggactcgtta gagcattgcc tataactgat
     4441 ctgtgccagt ttttacctaa gggtgctttg tatttagagt tgttcaattt tatagttatt
     4501 tctcttaaac tagtaccttt tttcgcttct tctactgcaa atcgtaatac ttttttatat
     4561 tcattaggca caaatttatc gtttactctg tcgtaataga aaggagggac agttttagct
     4621 aacccttttc tagctgatgc gcgtcgaccc attgcagtac gctcttgaat tgtagtacgc
     4681 tcccactctg ccatagcacc tactaatgtt acgaacaaac gtcccatagc agaagttgtg
     4741 tcatatactt ctgttgcgct cctaaacaac acgtttttat tctcaaacaa ttctagtatc
     4801 tctagtaagt ctttaacact tcgagttaat cgatctagtt tatagactaa aaccaaatca
     4861 aaattatcta tttcattcaa catttcttgt aaagcgggtc tgtctttttt agctccggag
     4921 tatccagcgt cagtatatac tttatgaatt ttccagtcgt ttatgtcgct gtaagctctt
     4981 aattttcttt cttgttcttc gatagagtgt cctttttctt tttgttcaag tgtactcact
     5041 ctagtataaa ttgctacttt catgtgctcc ctcctcaaaa ttggcaaaaa ataataaggg
     5101 taggcgggct acccgtgaaa attgtataaa aaaagagaga gcgcagatgc accctctcat
     5161 gtcgcaaata tttcagcgac ttgtctaatt tgaagcttgc cgcaaatatt tcagcggctt
     5221 gttttgtata tatgtaatat accatcaaag agagtgtagt tcaagcgatt taactaagaa
     5281 atctaatttt tatactattt tcaattttat ctactgtttc ttttgaatat gatatttctc
     5341 cggcagggtc atacctatta attttcgata ttctatcctt gctgattgta gtgatattta
     5401 aaacgttagc ataggtcttt ttatacttga atcgctcata tcttttgcga accttcgaat
     5461 attttttgaa gtcgtcattc agcgatttgt tttcatcaag taatttttga tcgtatgggt
     5521 tttctgcttt tgacaccttt tcaagattgt tcatgatttt tttagctaaa tccttacccg
     5581 ttacgtccat tttttccaat actaaaggta acaaatcttc ttcgatatgc acattgaatt
     5641 tacttctgga agatgtaagt ggaactaccg ttaatattgg atttttattt gaatcgtgat
     5701 tattaagtac catacaaaaa tggtttccag aaaactctct gccaacatta acacctaact
     5761 ttacataaat tatagtgcct tttttatatc tggtgtaact tttgttttct tttaacaatc
     5821 taacttcatc caataaaaac tctgaatatt caagacacca tgaattcata tatttaaatt
     5881 tgtaaatctc gctattttga atctttttaa aattattaac tgctgtttct aaaggtgcgt
     5941 tctcttccat ccctcatcct cctcacgcca cacaggcgct gttaatcaat atgatgcaat
     6001 ttaaaaactc tcaacggctc aaatgtaata gaatactcgc cgtagtgggt accaatacca
     6061 tatatctttt tatattgttc tattgcttct aatatgtatt cttcacttaa ttgtagatac
     6121 tcagacaact catacaagtt acgtacgcca taattataag cttctacaat ttcgcgtaat
     6181 ggaacagctg agataaagcc gtgtcgtctt gcgtaatttt caaacttgcg attgttgaat
     6241 ttcgattgat ctaaaatgtt gccatacgtc aacttgtggt gggcaagttc ttcatataat
     6301 acttctaatt tgttcctttc ggatagggaa ggtctaataa aaatttctcc ttcttgatac
     6361 caaccatcga atcctcgagg tactctttgt gtttctttca cttcaacttc acatttcata
     6421 agcaattctt cgtattttcc catgcgccaa acccctttgg tgtcttattt ctttctatct
     6481 ctaacccatt gcataaagtt ttcgatttct tcccattctt cgggagtaaa ttcatcttta
     6541 tttgcatgac cggctatagt ttcttgatga atacttcttt cttctgtaat tctcgattta
     6601 ggtacattaa agtaatctgc taattgttgg acttttgata ttctaggata tttaagttct
     6661 ttaagccagt tagagattgt tgattgactt accccgattg cttcagacaa ttctacttga
     6721 gtaatgttgt tctctttcat aagttgttct aagttctctg ataaaatttt tctagcactc
     6781 ttatattcca taattttctc ctttagtatt acttaatgta atactaattt accataagta
     6841 atatcacttt tcaatacaaa atattacttt tttgaaataa atatcacttt aggtgttgac
     6901 atattacttt aagtgatagt atagttgtaa atgtcaacag gaggtgaaac gaaatgccgg
     6961 aagatttcaa agaattctca gtgaaagttt ggaggactaa ttcaaatatg acacaacagg
     7021 atgttgctga taaattaggc gtcactaagc aatctgtcat aagatgggaa aaagatagta
     7081 cagagttaaa aggtttacag ttatacgctt tagccaaatt attcaacact gaagttgatt
     7141 acataaaggc taaaaaaatt taatacaaat atcgctttaa gttatattag gaggtggatt
     7201 aaatgcttat agatatcaat aaggtaactg tcggcaaacg cataagaaaa gattacggag
     7261 atataacatc acttgctgat gatattgaag acaggggttt aattaatccg ccggttgtca
     7321 cgccggatta tgaattaata gctggagaac gaagattaaa agctatgaag aaacttgatt
     7381 ataggcaaat agaagtgagg gttatgtccg tagaagatta tgaacatcaa ctcaaaatag
     7441 aaattagtga aaatgaagag cgaaaagctt ttacttattc agaaagaatg gattacgcga
     7501 aacaattaga aagaattgaa gctaaaaaag ctaaagatag aaagacttct aagctaaaac
     7561 aaaacaaaga caccgttacg gaccaaggac cggaacggaa aggagaaact cgagatatcg
     7621 tcggtaaggc ttctggtttt ggcagtggac gaacatatgc aagagctaaa tacatctatg
     7681 aaaacgcaga cgaagaaact ataaaagaag ttgatgaagg taaaaaaagt attcgcaaag
     7741 cgcatgatga acttcgtgct aaagaaaagc aaaatgaggc aaataaagtg aaagtgacaa
     7801 caaaagaaaa acctcaaacg acagtagtag atcgaagaga acggatgaac gcagaagttg
     7861 aagctatgag tgaaacagaa acaaatttag ctttgtctga agctgcagct gcaaacatag
     7921 taaatgtctg ttctaactta ttatacgcgg taaacaacat tgaagattta gagttaactt
     7981 tgaatttctt gaaatcaaga gatgctgaag aattatcaaa agttgtcaag gcttctaaag
     8041 ctttaaacaa aattatagaa aaaggagatt ttataaatgt ataaccagca attaccagtt
     8101 cacagattac agacagatat gagttatcaa tcaccggtgc aagaaagaca agtaagaaag
     8161 atagttaata aatttgattc aaaaaaatta cacacaattg tagttagtaa aagaaaagat
     8221 gggtcatttt atatcatcga tggtcaacat agggtggaag cgttgaaaga attgaatatt
     8281 tcatttatag aagcgacagt gcacgaaaac ttaactattg aagaagaagc tgagatgtat
     8341 tacggagtta atgatagacc ttcgaaaaac gcaaactcta aaggtaaatc gcgacttaga
     8401 tttaaagagc cggtagctgt agctatagat gaaacggtaa aaagtgtcgg gttggaaatt
     8461 gattatgaaa aaagcgcatc aacaaaagga tatatcaaag cttatgatgc gttgcaatca
     8521 atatacaaag gaaatggtgc taaccattta ggtttagttt tggaaataat taaagattct
     8581 tttggagagg atacaaggaa ttatcaatca tttattttaa gaggattctc taaattattg
     8641 aaagtatacc tgcatgaagt ggatttaaat tttttggtta aaaaacttca gaacattggt
     8701 tacgaaggtt ttattttaga gattaataag aaacatgctg gatttaaaac aaaaaaagaa
     8761 tgcctaccat ttgtagtagt agacatttac aacaaaaatc gtcgccaaaa aaatcaattg
     8821 gacaaaatga aattacatgt ttaatcaaca acacaattat accatacgag gtggttgtga
     8881 gccacctcaa acatcttaaa aggaggaaca acaaatggaa caaacaatca aacaattttt
     8941 agaatttaga aagcaattca cacccgcaca gtggcacgaa atcaacagaa ttattgacgg
     9001 acaatttagt aaaaaagccg ccgagctaca actcgacgac caagatgttg aggttattaa
     9061 aaatattatt actcaacaaa agattatgaa gtaacaattt gaataaaagt tattcaaaaa
     9121 tcacgaaagg aagatacaaa atgatgctga ccaacacact actagcaatt cactttttca
     9181 tgaatttagc gatattaatt atgctcgtaa gaatcggtag agattaactt atacttttta
     9241 agtttgttta ttcgttgatt agaaacaatc tttataaacg caggttctaa ttcgaattta
     9301 tataaaaact ctgatgacga attagctatc ataacttctg gtttatctaa ttgtttatcg
     9361 attggagcat gcaaataact tggattatat ataccgagag atgaatattc atcagattcc
     9421 aatactacgt taataggtgt taaaacattt ttgtttttat ctaaaaatat taattcttct
     9481 aaagtatgag ttctagacga gtcattagcg acgacaaaat ttaattctac aaaattattt
     9541 tcatagtaga aattaagatc gctaataaca aaactgaact tattttgaga acgagtataa
     9601 aaaattgagt atgcagatag acctaaagct agaaaagcta caacgtttga aaacaaagta
     9661 gattgtatga attccataag aataaccacc ttaaatattt gataacaaca ttatacacga
     9721 aaggagcata aatattatgc aagcattaca aacatttaat tttgaagaat taccagtaag
     9781 aacattaaca gtaaatgagg aaccgtattt tgtagggaaa gatgtagcag atattctagg
     9841 ttacaaaaat ggcagtcgtg atattaacgc tcatgttgat gcagaagata agctgacgta
     9901 ccaaatcagt accgcaggtc aaagacgaaa tcaaacaatc atcaacgaat cgggtttata
     9961 cagcctaatc ttctcatcaa aactagaatc agctaaacga ttcaaacgtt gggtaacatc
    10021 agacgtccta ccagccattc gcaaacacgg tatctacgca acagacaatg taattgaaca
    10081 aacattaaaa gatccagact acattattac agtgttgact gagtataaga aagaaaaaga
    10141 gcaaaactta cttttacaac aagaaatcgg agaactaaaa cccaaagcag actatgtaga
    10201 tgaaatctta aagtcaactg gcacattagc cacaactcaa atcgcggcag actacggtat
    10261 atcagcacaa aagttaaaca aactactaca cgaagctaga ctacaacgaa aagtaaataa
    10321 acagtgggtg ctttactcag aacacatggg caagagttac acagattcag acactataac
    10381 aattgtgcgt tctgatggca gagaagacac agttttacaa actagatgga cacaaaaagg
    10441 cagattgaaa atacatgaaa tcatgactga attcggttat gaagctaatt tagggggagc
    10501 gtaaatgaca ccagaacaaa aagaaaagct aaacaatata gtattaacac tttatgcagt
    10561 taaagaaaac aaaagtcaaa catacacaca caaagatact cttactgtga catatgcagg
    10621 cgagattgag cacacttacg aagtcgacag agagaaacac cttgaatcaa tgattgagtg
    10681 ggcaattgac caaatcgaac agcactttga tttagacgaa gaagaataac acacaattga
    10741 acaaacaact taataggagg aactacaaat gttacaaaaa tttagaatcg ctaaagaaaa
    10801 aagtaaatta aaactcaatt tactaaaaca tgcaaacagt aatttagaaa caagaaacaa
    10861 ccctgaactg ttgcgagcag ttgcagagtt gcttaaagag attaatcgat aaattagaga
    10921 actaatcatc gtttcagttc tcgtcatcta agtaattagc aattacgtaa agtgttaaaa
    10981 gagttgctat tacattaaaa aacataaagt agttcacata ttggtcaagt atcgttctaa
    11041 ccataaccgg agatgttact ccgatagcag aaccgacaga aacctttttt ataaattttg
    11101 cagaaggaga aataaaaata tgattgagcg attcattgaa tacactattg tatttcttag
    11161 tatttacgtc atctattgga tgggcagaat cgacggtttt accaagaaca gggacatcga
    11221 cagtatcgac aaaagaattt ctcaaatgag tgctaacttt gcggacttca tcctccggaa
    11281 agttgttaga acttatgaat tcatcaagaa ttttttcaga aaataaatta gttttgaaca
    11341 ttggatgatt cttagttact cgatgcatat aggaagccca atcagataat ttagattggt
    11401 taattctaat gctattcata acattattaa ccgtcgattg aatttccaga gcgttcataa
    11461 catacgaatt attcatagta tttgtggctt tagcgtaagc ttcgacaggc aatttagata
    11521 agatagcttt atttttcttt attaaatcta actgtcgttg agtgagattt atattattca
    11581 taattaccac ctcctttcac taggagataa ctaaattata cacgaaagga atggtagaag
    11641 tgccaccaca cattcaacaa atgttatacg aaatccagtt aaaagctggt atacctcaaa
    11701 aattaatgga aatgcaaggt ttgataaacg atgaaacaac caaagaggag aaaaaagaaa
    11761 atgagtaaca tttataaaag ctacctagta gcagtactat gcttcacagt cttagcaatt
    11821 gtgcttatgc cattgctgta cttcactaca gcatggtcaa ttgcgggatt cgcaagtatc
    11881 gcaacattca tgtactacaa agaatgcttt ttcaaagaat aaaaaaactg ctacttgttg
    11941 gagcaagtaa cagtatcaaa cacttaagaa aaaattcatg ttcaatataa aacgaaaaac
    12001 ggaggaagtc aagatgtatt acgaaatagg cgaaatcata cgcaaaaata ttcatgttaa
    12061 cggattcgat tttaagctat tcattttaaa aggtcatatg ggcatatcaa tacaagttaa
    12121 agatatgaac aacgtaccaa ttaaacatgc ttatgtcgta gatgagaatg acttagatat
    12181 ggcatcagac ttatttaacc aagcaataga tgaatggatt gaagagaaca cagacgaaca
    12241 ggacagacta attaacttag tcatgaaatg gtaggaggtc gctatgaagc agactgtaac
    12301 ttatatcatt cgtcataggg atatgccaat ttatataact aacaaaccaa ctgataacaa
    12361 ttcagatatt agttactcca caaatagaaa tagagctagg gagtttaacg gtatggaaga
    12421 agcgagtatc aatatggatt atcacaaagc aatcaagaaa acagtgacag aaactattga
    12481 gtacgaggag gtagaacatg actgaggaaa aacaagaacc acaagaaaaa gtaagcatac
    12541 tcaaaaaact aaagataaat aatatcgctg agaaaaataa aaggaaattc tataaatttg
    12601 cagtatacgg aaaaattggc tcaggaaaaa ccacgtttgc tacaagagat aaagacgctt
    12661 tcgtcattga cattaacgaa ggtggaacaa cggttactga cgaaggatca gacgtagaaa
    12721 tcgagaacta tcaacacttt gtttatgttg taaatttttt acctcaaatt ttacaggaga
    12781 tgagagaaaa cggacaagaa atcaatgttg tagttattga aactattcaa aaacttagag
    12841 atatgacatt gaatgatgtg atgaaaaata agtctaaaaa accaacgttt aatgattggg
    12901 gagaagttgc tgaacgaatt gtcagtatgt acagattaat aggaaaactt caagaagaat
    12961 acaaattcca ctttgttatt acaggtcatg aaggtatcaa caaagataaa gatgatgaag
    13021 gtagcactat caaccctact atcactattg aagcgcaaga acaaattaaa aaagctatta
    13081 cttctcaaag tgatgtgtta gctagggcaa tgattgaaga atttgatgat aacggagaaa
    13141 agaaagctag atatattcta aacgctgaac cttctaatac gtttgaaaca aagattagac
    13201 attcaccttc aataacaatt aacaataaga aatttgcaaa tcctagcatt acggacgtag
    13261 tagaagcaat tagaaatgga aactaaaaat taattaaaag gacggtattt aattatgaaa
    13321 atcacaggac aagcgcaatt tactaaagaa acaaatcaag aaaagtttta taacggctca
    13381 gcagggtttc aagctggaga attcacagtg aaagttaaaa atattgaatt caatgataga
    13441 gaaaatagat atttcacaat cgtatttgaa aatgatgaag gcaaacaata taaacataat
    13501 caatttgtac cgccgtataa atatgatttc caagaaaaac aattgattga attagttact
    13561 cgattaggta ttaagttaaa tcttcctagc ttagattttg ataccaatga tcttattggt
    13621 aagttttgtc acttggtatt gaaatggaaa ttcaatgaag atgaaggtaa gtattttacg
    13681 gatttttcat ttattaaacc ttacaaaaag ggcgatgatg ttgttaacaa acctattccg
    13741 aagacagata agcaaaaagc tgaagaaaat aacggggcac aacaacaaac atcaatgtct
    13801 caacaaagca atccatttga aagcagtggc caatttggat atgacgacca agatttagcg
    13861 ttttaaggtg tggtttaaat gcaatacatt acaagatacc agaaagataa cgacggtact
    13921 tattccgtcg ttgctactgg tgttgaactt gaacaaagtc acattgactt actagaaaac
    13981 ggatatccac taaaagcaga agtagaggtt ccggacaata aaaaactatc tatagaacaa
    14041 cgcaaaaaaa tattcgcaat gtgtagagat atagaacttc actggggcga accagtagaa
    14101 tcaactagaa aattattaca aacagaattg gaaattatga aaggttatga agaaatcagt
    14161 ctgcgcgact gttctatgaa agttgcaagg gagttaatag aactgattat agcgtttatg
    14221 tttcatcatc aaatacctat gagtgtagaa acgagtaagt tgttaagcga agataaagcg
    14281 ttattatatt gggctacaat caaccgcaac tgtgtaatat gcggaaagcc tcacgcagac
    14341 ctggcacatt atgaagcagt cggcagaggc atgaacagaa acaaaatgaa ccactatgac
    14401 aaacatgtat tagcgttatg tcgcgaacat cacaacgagc aacatgcgat tggcgttaag
    14461 tcgtttgatg ataaatacca cttgcatgac tcgtggataa aagttgatga gaggctcaat
    14521 aaaatgttga aaggagagaa aaaggaatga atagactaag aataataaaa atagcactcc
    14581 taatcgtcat cttggcggaa gagattagaa atgctatgca tgctgtaaaa gtggagaaaa
    14641 ttttaaaatc tccgtttagt taatacaggt ttttacaaaa gctttaccat aggcggacaa
    14701 actaattgag ccttttttga tgtctattac ccaggggctg taatgtaact ttaatacttc
    14761 aaattcaatg ccagaaagtt tacttattgt ttctaggttg tgtcctgact ttaacattct
    14821 tttaacaaat tctaatcccg aaacaaatct ttgtttttct ataatcttat taaagtgatt
    14881 taaaaactga ggagcataaa acttattata aattcctttt tttgttaagt aagacatgtc
    14941 aaaagtttca tttaaaaccc ctaaccttac taggttatta attgaaattt cggttgattc
    15001 tatatctaac ggagagtctt ttattaacgt gtccgatata ttcataccgt cattctttgg
    15061 gtttaaaacc gctctatatt taacggcagg atgtacttcg tgattcttta aatgttttaa
    15121 aagaatagca tcatttgggg ataattgttt aattatttca acaaatgaat ggtgggttaa
    15181 tgagtttttt ctgtcatcca tagatgatgc tattagtttt gcgaacatat tacttaaagt
    15241 tttttcacta atgtaaaact ttgaagcttc tagagcagga cctagaagag aaaattgtgg
    15301 ttcttgtaaa ttattttcag gtacagaaga tatttctttt ttaaattgtt ctttgaattt
    15361 ttcaaattct acttctcttt gataaataac tttatccaca taaaggtgga atttcccaaa
    15421 gacaagttcc caagttttag agaatgtttc tacaggccct tttgatgcgc cttcaataat
    15481 tttatcaata cctttaccta aaataggatc cataattatt cacccccaat ctaacgcagt
    15541 agcgataaca aaattatacc agaaaggaga atcaacatga ctgaccaacc aagttactac
    15601 tcaataatta cagcaaatgt cagatacgat aaccgactta ctgacagcga aaagttactt
    15661 tttgcagaaa taacatcttt aagtaacaaa tacggatact gcacagcaag taatggttac
    15721 tttgcgaaac tatatgaagt tacaaaagtt actgtatcac gccgtatagc caacttaaaa
    15781 gaatgtggat atttacatgt tgaaatcatt agaaatggta atgaaattaa acaaagaaag
    15841 ctatacccct taacagaaat gataagacct attaacacaa atgataatac ccctattaac
    15901 aattctgtta atacccctat tatcacaaat gttaaagaga ataatacaag tattaataat
    15961 acaagtaata acaatataaa tagagtagat atattgtcgg gaaacccgac acgcatccca
    16021 tataaagaga ttattgatta tcttaacgaa aagactggca agaagtttaa acataataca
    16081 actaaaacga aagattttat taaagcaagg tggaatcaag attttaggtt ggaggatttt
    16141 aaaaaggtaa ttgatattaa aacagctgag tggcttaaca cggatagcga taaatacctt
    16201 agaccagaaa cactttttgg caataaattt gagggatacc tcaatcaaaa agtacaacca
    16261 actggcacgg atcaattaga acgcatgaag tacgacgaaa gttattggga ttagagggat
    16321 attatgaaac aactattcag cgaaaagata aacgaaagct taaaaaaata tcaacctact
    16381 catgtcgaaa aaggattgaa atgtgagaga tgtggaagtg aatacgactt atataagttc
    16441 gctcctacta aaaaacaccc gaatggttat gagtataaag acggttgcaa atgtgaaatc
    16501 tatgaggaat ataagcgaaa caagcaacgg aagataaaca acatattcaa tcaatcaaat
    16561 gttaatccgt ctttaagaga tgcaacagtc aacaactaca aaccacaaaa tgaaaaacaa
    16621 gtacacgcta aacaaacagc aatagagtac gttcaaggct tctctacaaa agagccaaaa
    16681 tcattaatat tgcaaggttc atacggaact ggtaaaagcc acctagcata cgctatcgca
    16741 aaagcagtta aagctaaagg gcatacggtt gcttttatgc acataccaat gttgatggat
    16801 cgtatcaaag cgacatacaa caaaaatgca gtagagacta cagacgagct agtcagattg
    16861 ctaagtgata ttgatttact tgtactagat gatatgggtg tagaaaacac agagcacact
    16921 ttaaataaac ttttcagcat tgttgataac agagtaggta aaaacaacat ctttacaact
    16981 aactttagtg ataaagaact aaatcaaaat atgaactggc aacgtatcaa ttcaagaatg
    17041 aaacacaatg caagaaaagt aagagtaatc ggagacgatt tcagggagcg agacgcatgg
    17101 taaccaaaga atttttgaaa attaaacttg agtgttcaga tatgtacgct cagaaactca
    17161 tagacgaggc acagggcgat gaaaataagt tatatgacct atttatccaa aaacttgcag
    17221 aacgtcatac acgccccgct atcgtcgaat attaaggagt gttaaaaatg ccgaaagaaa
    17281 aatattactt ataccgagaa gatggcacag aagatattaa ggtcatcaag tataaagaga
    17341 atgagaatga agtttattcg ctcacaggag cccatttcag cgacgaaaag aaaattatga
    17401 ctgatagtga cctaaaacga tttaaaggcg ctcacggact tctatatgag caagagctag
    17461 gtttacaagc aacgatattt gatatttaga ggtggacgat gagtaaatac aacgctaaga
    17521 aagttgagta caaaggaatt gtatttgata gcaaagtaga gtgtgaatat taccaatatt
    17581 tagaaagtaa tatgaatggc actaactatg atcgtatcga aatacaaccg aaattcgaac
    17641 tacaacctaa atttgggaaa caaagaccga ttacgtatat agctgatttc tctttgtgga
    17701 aggatggcaa actggtcgaa gttttagatg ttaaaggtaa ggcgactgaa gttgccaaca
    17761 tcaaagcgaa gatattcaga tatcagtata gagatgtgaa tttaacgtgg atatgtaaag
    17821 cgcctaaata cacaggtcaa gaatggatgg tatatgagga cttagtgaaa gtcagacgta
    17881 aaagaaaaag agaaatgaag tgatttaatg caacaacaag catatataaa tgcaacgatt
    17941 gatataagaa tacctacaga agttgaatat catcatttcg atgatgtgga tgatgaaaaa
    18001 gatatgctag caaagcgctt agatgacaat ccggatgaat tactaaagta tgacaacata
    18061 acaataagac atgcatatat agaggtggaa taaatggcga aaacagcaag aattgtaagg
    18121 atacacgata aaccttatag gttcagtaaa tttgaaatgg aattaataga aagtcacggt
    18181 ataaccgctg gaatggtttc taagagagta aaagacggtt gggaactaca tgaagcaatg
    18241 gacgcaccag aaggtacgcg tttaagcgag tacagagaaa agaaaacaat agaaagactg
    18301 gaacaagcta gactcgaacg caaattggaa agaaagcgaa agagagaggc tgagctaaga
    18361 agaaagaagc cacacttgtt taatgtacct cagaaacatt cacgtgatcc gtactggttt
    18421 gataatactt ataaccaaat gttcaagaag tggcaggaag tataaatgcc taaaaccgat
    18481 agcgcatgta aagaatactt aaaccaattt ttcggctcta agagatattt gtatcaggat
    18541 aacgaacgag tggcacatat ccatgtagtg aatggcactt attactttca cgggcatatc
    18601 gtaccaggct ggcaaggcgt gaaaaagaca tttgatacaa ccgaagagct cgaaacatat
    18661 ataaagcaac atggtttgga ata
//
LOCUS       DANBWH010000028        18630 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.028, whole genome shotgun sequence.
ACCESSION   DANBWH010000028 DANBWH010000000
VERSION     DANBWH010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18630
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.028"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..338
                     /locus_tag="PPY92_000682"
     CDS             <1..338
                     /locus_tag="PPY92_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDJ2624705.1"
                     /translation="LFLDTIFRLMLPFSTYIFILIPLLGLTYMTQSILNIAIITLRQK
                     KCSENMLGRVNSVFKTMVFAFRPIGLFLGGILLENKGGFYALTISAVLFIPLVLYILK
                     NRFYQVEEY"
     gene            805..1176
                     /locus_tag="PPY92_000683"
     CDS             805..1176
                     /locus_tag="PPY92_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDJ2624706.1"
                     /translation="MEKLNQVMLYVDDVEKAKAFWTETLEFVVVSETPLAEDYVAVEV
                     SPTKDAETSLTIMAKEFIEKHSPEVNLGTPSLMFKEKNFDALYSKLNDLGLTGHDIVE
                     MDGQRVFNFQDGQGNYFAVSD"
     gene            1115..1369
                     /locus_tag="PPY92_000684"
     CDS             1115..1369
                     /locus_tag="PPY92_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624707.1"
                     /translation="MVNVYLISKMDKVIILQLVTKQSILIFIQTFILIFGLNFSMKVL
                     FLSFYAIVCTNLLTFLERVTKLLLYRGWKRFPMQKLTGGD"
     gene            1370..2389
                     /locus_tag="PPY92_000685"
     CDS             1370..2389
                     /locus_tag="PPY92_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDJ2624708.1"
                     /translation="MVTIKDVALKAGVSPSTVSRVIKGNKRISEATISKVKKVMEELN
                     YFPNTAARTLITNQTYKIGLVLKGSEEPIRLNPFYINVLLGISETCNQHGYGTQTTVS
                     NNMNDLMDEVYKMIKQRMVDAFILLYSKENDPIKQMLIDESMPFIVIGKPTSDIDHQF
                     THIDNDNILASENLTRHVIEQGVDELIFITEKGNFEVSKDRIQGFETVASQFNLDYQI
                     IETSNEREVILNYMQNLHTRLKDPNIKQAIISLDAMLHLAILSVLYELNIEIPKDVMT
                     ATFNDSYLTEIASPPQTCIDIKPRMLGQQAGSAILNILKNKAQDVIELVIIDTELKIR
                     KSTQR"
     gene            2405..4054
                     /locus_tag="PPY92_000686"
     CDS             2405..4054
                     /locus_tag="PPY92_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDJ2624709.1"
                     /translation="MNKQWWKEAVAYQVYPRSFNDSNHDGIGDLPGMIDKLDYLKDLG
                     IDVIWLSPMFKSPNDDNGYDISDYQEIMDEFGTMEDFDCLLKGIHDRGMKLILDLVVN
                     HTSDEHPWFIESKSSKDNPKRDWYIWQDPKQDGSEPNNWESIFNGSTWEYDANTEQYY
                     FHLFSKKQPDLNWGNPEVRDAVFEMMNWWFDKGIDGFRVDAITHIKKTFEAGDLPVPE
                     GKTYAPAFDVDMNQPGIQTWLQEMKDRSLSKYDIMTVGEANGVSPDDADDWVGEENGK
                     FNMIFQFEHLGLWNSGDSHFDVNSYKSVLNRWQKQLENKGWNALFIENHDQPRRVSTW
                     GDDDKYWYESATSHAAVYFLQQGTPFIYQGQEIGMTNYPFESIETFNDVAVKNDYQIV
                     KAQGGDVDALLAKYKDENRDNSRTPMQWDDTLNGGFTNGEPWFPVNLNYKTINVAQQL
                     EDEHSVLQFYKDLIQLRKSNDVYVYGQFDLVDAENSQVFAYTRTLNEKQVLIVGNLTN
                     HEAELTVPFDLSHGEVKLFNYDAKVNLKQLRPYEACVIELN"
     gene            complement(4136..5002)
                     /locus_tag="PPY92_000687"
     CDS             complement(4136..5002)
                     /locus_tag="PPY92_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDJ2624710.1"
                     /translation="MDVIKQIQQAIVYIEDRLLEPFNLQELSDYVGLSPYHLDQSFKM
                     IVGLSPEAYARARKMTLAANDVINGATRLVDIAKKYHYANSNDFANDFSDFHGVSPIQ
                     ASTKKDELQIQERLYIKLSTTERAPYPYRLEETDDISLVGYARFIDTKYLSHPFNVPD
                     FLEDLLIDGKIKELRRYNDVSPFELFVISCPLENGLEIFVGVPSERYPAHLESRFLPG
                     KHCAKFNLQGEIDYATNEAWYYIESSLQLTLPYERNDLYVEVYPLDISFNDPFTKIQL
                     WIPVKQSPYDED"
     gene            complement(5232..6716)
                     /locus_tag="PPY92_000688"
     CDS             complement(5232..6716)
                     /locus_tag="PPY92_000688"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDJ2624711.1"
                     /translation="MSTKNKHIPCLITIFGATGDLSHRKLFPSIFHLYQQDNLDEHIA
                     IIGIGRRDITNDDFRNQVKSSIQKHVKDTNKIDAFMEHVFYHRHDVSNEESYQELLDF
                     SNELDSQFELKGNRLFYLAMAPQFFGVISDYLKSSGLTDTKGFKRLVIEKPFGSDLKS
                     AEALNNQIRKSFKEEEIYRIDHYLGKDMVQNIEVLRFANAMFEPLWNNKYISNIQVTS
                     SEILGVEDRGGYYESSGALKDMVQNHMLQMVALLAMEAPISLNSEDIRAEKVKVLKSL
                     RHFQSEDVKKNFVRGQYGEGYIDGKQVKAYRDEDRVADDSNTPTFVSGKLTIDNFRWA
                     GVPFYIRTGKRMKSKTIQVVVEFKEVPMNLYYETDKLLDSNLLVINIQPNEGVSLHLN
                     AKKNTQGIETEPVQLSYSMSAQDKMNTVDAYENLLFDCLKGDATNFTHWEELKSTWKF
                     VDAIQDEWNMVDPEFPNYESGTNGPLESDLLLARDGNHWWDDIQ"
     gene            7029..7949
                     /gene="rnz"
                     /locus_tag="PPY92_000689"
     CDS             7029..7949
                     /gene="rnz"
                     /locus_tag="PPY92_000689"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDJ2624712.1"
                     /translation="MEVTFFGTSAGLPTKERNTQAIALNLEPYSNSIWLFDVGEGTQH
                     QILHHAIKLGKVTHIFITHMHGDHIFGLPGLLSSRSFQGGEQKPLTLVGPKGIKAYVE
                     MSMNLSESHLNYPITYIEIDDHLTYHHDGFTVEAHLLNHGIPSYGYRVMAPETTGTIN
                     VEALKNIGLEPGPKYQEVKSHDTFEHNGQVYQSKDFRGESKQGPVVAIFGDTKPCSNE
                     RVISRDADVMVHEATYIDGEKHLANNYHHSHIEDVFALIKEANVKRTLITHLSNRYNT
                     EDINEIYQILIQNEDTPNFNFVKDFDSFKV"
     gene            complement(8052..8867)
                     /gene="proC"
                     /locus_tag="PPY92_000690"
     CDS             complement(8052..8867)
                     /gene="proC"
                     /locus_tag="PPY92_000690"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDJ2624713.1"
                     /translation="MKLVFYGAGNMAQAIFTGIINSSNLDANDIYLTNKSNEQALKAF
                     AEKLGVNYSYDDATLLKDADYVFLGTKPHDFDALATRIKPHITKDNCFISIMAGIPID
                     YIKQQLECQNPVARIMPNTNAQVGHSVTGISFSNNFDPKSKDEINDLVKAFGSVIEVS
                     EDHLHQVTAITGSGPAFLYHVFEQYVKACTKLGLEKEQVEESIRNLIIGTSKMIERSD
                     LSMAQLRKNITSKGGTTQAGLDTLSQYDLVSIFEDCLNAAVDRSIELSNVEDQ"
     gene            9009..9764
                     /locus_tag="PPY92_000691"
     CDS             9009..9764
                     /locus_tag="PPY92_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDJ2624714.1"
                     /translation="MKGQHFIVTGGTSGLGLSIVRKLLENKVHVTLLVRDVDKATRIF
                     EQELGKTINVIPCDLNDLKSIQALQFEDNTSFDGFIYSAGLGYFKSISDHSFSEMIET
                     YQLNLISFNVLYTVLRPYLTSNAHIVGISSQAAFSTQANAAHYGASKAGFYALMNALR
                     LESPNLHIMTVNVGPIDTPFHQKADPSMKYAKKMGEIMLDANQLAEDIIYGIKTKQLE
                     INRPKWMHHALKMYQIAPRFFERCFPKLFKNKA"
     gene            9780..10028
                     /locus_tag="PPY92_000692"
     CDS             9780..10028
                     /locus_tag="PPY92_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624715.1"
                     /translation="MNCQSESVVRLCVRYAEQLSVFEEFTVLDILGDISVDQISDGTL
                     YYTCEKFKLLVLQGNVLGVQIITNNDESTCEVKYRKMF"
     gene            complement(10244..11152)
                     /locus_tag="PPY92_000693"
     CDS             complement(10244..11152)
                     /locus_tag="PPY92_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDJ2624716.1"
                     /translation="MQKNILKSGIALSELGLGCMSLGTDLKKAEQIIDCAVENGITYF
                     DTADMYDKGINESVVGKALLKYQQRDDIFIGTKVGNRLTKDGSTTWDPSKSYIKEAVK
                     GSLKRLGIDHIDLYQLHGGTIDDPLDETISAFDELKQEGIIRAYGISSIRPNVIDYYL
                     KHSQIETIMSQFNLIDNRPESLLDAIHNNDVKVLARGPVSKGLLTSNSVNVLDNKFKD
                     GIFDYSHDELGETIASIKEIESNLSALTFSYLTSHDVLGSIIVGASSVDQLKENIENY
                     HTKVGLDQIKTARARVKDLEYTNHLV"
     gene            11234..11776
                     /locus_tag="PPY92_000694"
     CDS             11234..11776
                     /locus_tag="PPY92_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDJ2624717.1"
                     /translation="MDLNEKTIDRTVIYNGKIVDVEIHTVTLPNGETSTRELVYHNGA
                     VAVCALTPKKEVVLVKQYRKPVEKPLLEIPAGKLEDDENRVEAAKRELEEETGYIAKE
                     LTHVVDMYGSPGFCDEQLSIYFTDNLEEGTVHLDEDEFVEVIKVPIENVKSMLMNKEI
                     EDAKTIIALQHLLLNYNHSK"
     gene            11881..12330
                     /locus_tag="PPY92_000695"
     CDS             11881..12330
                     /locus_tag="PPY92_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDJ2624718.1"
                     /translation="MEERLNRVKQQLQQSSYKLTPQREATVRVLIENEKDHLSAEDVY
                     LKVKDKAPEIGLATVYRTLELLAELKVVDKINFGDGVARFDLRKEGAKHFHHHLVCME
                     CGRVDEIDEDLLPEVENRVENEFNFKILDHRLTFHGVCETCQAKGKG"
     gene            12379..13266
                     /gene="xerD"
                     /locus_tag="PPY92_000696"
     CDS             12379..13266
                     /gene="xerD"
                     /locus_tag="PPY92_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDJ2624719.1"
                     /translation="METIIEEYLRFIQIEKGLSSNTIGAYRRDLKKYQDYMTEHHISH
                     IDFIDRQLIQECLGHLIDQGQSAKSIARFISTIRSFHQFAIREKYAAKDPTVLLDSPK
                     YDKKLPDVLNVDEVLALLETPDLNKINGYRDRTMLELLYATGMRVSELIHLELENVNL
                     IMGFVRVFGKGDKERIVPLGDAVIEYLTTYIETIRPQLLKKTVTEVLFLNMHGKPLSR
                     QAIWKMIKQNGVKANIKKTLTPHTLRHSFATHLLENGADLRAVQEMLGHSDISTTQLY
                     THVSKSQIRKMYNQFHPRA"
     gene            complement(13344..13850)
                     /locus_tag="PPY92_000697"
     CDS             complement(13344..13850)
                     /locus_tag="PPY92_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HDJ2624720.1"
                     /translation="MQQALINFYYQFHTKQHYFLCHDILEDAWKAENNYSKQDAVVSL
                     ILFATACYHYRRNNLKGAYKSFNKSKEIIQNAKDRDALYLNLNDYQLLIEQQIAKLNA
                     AKPFSSVILPITPVFERIIKANYPDYDYNQETATDPFIVDHHMRRDRSEVIAAKEEAI
                     QLRKHRRN"
     gene            13942..14673
                     /locus_tag="PPY92_000698"
     CDS             13942..14673
                     /locus_tag="PPY92_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDJ2624721.1"
                     /translation="MYEVKLDAFNGPLDLLLHLIQKFEIDIYDIPMQALTEQYMQYVH
                     AMKQLEINIASEYLVLASELLMIKSKMLLPQSTSDMDVDDDPREDLVGRLIEYQNYKE
                     YTAILNDMKEERDFYFTKRPTDLSHLETDESWDPNHTIDLTELIVAYQRVKNRVELNT
                     PKSVEIRKETFTIQQATEQVTSRLKDKDHFNFFSLFTFSEPIEQVVTHFLAILEMSKA
                     GIINIEQQRNFEDINIIRGVNYHFG"
     gene            14666..15208
                     /gene="scpB"
                     /locus_tag="PPY92_000699"
     CDS             14666..15208
                     /gene="scpB"
                     /locus_tag="PPY92_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDJ2624722.1"
                     /translation="MDNHGILESLLFTAGDEGLDEKQLLEILDMSKDQLVELIENYSS
                     HGLMIQRFGTTYVLTTKKEAATYIEQLIEQKSQMKLSQAAMEVLSIIAYNQPLSRSDI
                     ELIRSINSDGAVKTLIAKGLVEAKVVNEQRSQQLITTDLFLNVFGISNIEDLPTTEED
                     DEEMDAFFSNLVNQKGENND"
     gene            15201..15938
                     /locus_tag="PPY92_000700"
     CDS             15201..15938
                     /locus_tag="PPY92_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDJ2624723.1"
                     /translation="MTKELERLQKRIANSGYTSRRKAETLISEGKVKVNGTTVTELGT
                     KVKPSDTIEVEGIKIELEDKIYILFHKPTQVITSVSDDRGRTVVTDYFKDIEARIYPV
                     GRLDYDTSGLLLLTNDGEFTNLMTHPRYQIKKKYVAKLKGYLMREEVKALEKGIELED
                     GMTQPAEVKVKKQDKDKNTTLVEITITEGRNRQVRRMFEHFGHQVTKLSRIEYGPLNV
                     VGLNAGEGRVLTPHEVKVMRHLAEHGK"
     gene            16071..16796
                     /gene="srrA"
                     /locus_tag="PPY92_000701"
     CDS             16071..16796
                     /gene="srrA"
                     /locus_tag="PPY92_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator SrrA"
                     /protein_id="HDJ2624724.1"
                     /translation="MSNEILIVDDEDRIRRLLKMYLERESFEIHEASNGQEAYELAME
                     NNYACILLDLMLPEMDGIQVATKLREHKQTPIIMLTAKGEETNRVEGFESGADDYIVK
                     PFSPREVVLRVKALLRRTQSTTVEQSEPHARDVIEFKHLEIDNDAHRVLADNQEVNLT
                     PKEYELLIYLAKTPNKVFDREQLLKEVWHYEFYGDLRTVDTHVKRLREKLNRVSSEAA
                     HMIQTVWGVGYKFEVKSNDEPAK"
     gene            16777..18528
                     /gene="srrB"
                     /locus_tag="PPY92_000702"
     CDS             16777..18528
                     /gene="srrB"
                     /locus_tag="PPY92_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SrrB"
                     /protein_id="HDJ2624725.1"
                     /translation="MMSRLNSVVIKLWLTIILIVTTVLILLSIALITFMQYYFTQETE
                     NAIREDARRISSLVEQSHNKEEAIKYSQTLIENPGGLMIINNKHRQSTASLSNIKKQM
                     LNEVVNNDHFDDVFDKGKSVTRNVTIKEKGSSQTYILLGYPTKAQKNSHSKYSGVFIY
                     KDLKSIEDTNNAITIITIITAVIFLTITTVFAFFLSSRITKPLRRLRDQATRVSEGDY
                     SYKPSVTTKDEIGQLSQAFNQMSTEIEEHVDALSTSKNIRDSLINSMVEGVLGINESR
                     QIILSNKMANDIMDNIDEDAKAFLLRQIEDTFKSKQTEMRDLEMNARSFVVTTSYIDK
                     IEQGGKSGVVVTVRDMTNEHNLDQMKKDFIANVSHELRTPISLLQGYTESIVDGIVTE
                     PDEIKESLAVVLDESKRLNRLVNELLNVARMDAEGLSVNKEVQPIAALLDKMKIKYRQ
                     QADDLGLNMTFNYCKKRVWSYDMDRMDQVLTNLIDNASRYTKPGDEIAITCDENESED
                     ILYIKDTGTGIAPEHLQQVFDRFYKVDAARTRGKQGTGLGLFICKMIIEEHGGSIDVK
                     SELGKGTTFIIKLPKPE"
BASE COUNT         6554 a         2685 c         3348 g         6043 t
ORIGIN      
        1 tattattttt agatacaatt tttagattaa tgttaccatt tagtacttat atttttatac
       61 tcatcccact tttagggttg acatatatga cacaatccat tttaaatata gcgataatca
      121 cactgaggca aaagaaatgt tcagaaaata tgttaggacg tgttaatagt gtttttaaaa
      181 caatggtttt tgcattcaga cctataggcc tttttttagg cggtatatta cttgaaaata
      241 aaggtggatt ttatgcactt acgatcagtg ctgtgttatt tataccatta gtgttatata
      301 tactgaaaaa tagattttat caagttgaag aatattaaga agaaaattaa taaaaatggc
      361 tgctatgtca aattggactg atgaattcaa tataggaagt taggcaacta aacattgtat
      421 aactttcttt ttagttattg gagcttaaag ttttgaacat aataatattc gattgcgcaa
      481 atgattgtaa cttccataat caaatgatgt acgtttaatt aattttatta tgttagttat
      541 acattctaat ggacaatgtg agctacaccc agttaagttg acatagaaaa tacgaaaact
      601 ttgaacgaag tatttcattg atatatgtcc cagacgacat gtttgattaa tgaagttcta
      661 tgtggctaca aaattttaaa aattacacaa gcaatatcta ttaccttaaa agcatcccgc
      721 catattttta agcattttgt gaagtaataa aatatttact gtgttatgat agcatgtact
      781 atagagaaat agaggtacta ttttatggaa aagttaaatc aagttatgtt atatgttgat
      841 gatgttgaaa aagcgaaagc attttggaca gaaactttag aatttgttgt tgtaagtgaa
      901 acaccgttag ctgaggacta tgttgcagtt gaagtatcac ctaccaaaga tgctgaaacg
      961 tcattgacga ttatggcaaa agaatttatt gaaaagcata gtccagaggt gaacctagga
     1021 acaccatctt taatgtttaa agagaaaaac tttgatgcat tatactcaaa attaaatgat
     1081 ttaggtttaa ctgggcatga tatagtagaa atggatggtc aacgtgtatt taatttccaa
     1141 gatggacaag gtaattattt tgcagttagt gactaaacaa tcgattttaa tatttataca
     1201 aacctttata ttgattttcg gattgaattt cagtatgaag gttttatttt tatcgtttta
     1261 tgcaatcgtt tgcacaaatc ttttgacttt tttagaaaga gtaactaaac tgttattata
     1321 tagaggttgg aagcgttttc caatgcagaa attaacagga ggtgattaaa tggttacgat
     1381 taaagatgtt gcactaaaag ccggtgtttc tccttcgaca gtttcaagag ttataaaagg
     1441 aaataaacgt attagcgaag cgacaatttc aaaagtgaag aaagttatgg aagaattgaa
     1501 ttattttcct aataccgctg ctagaacttt aattacaaac caaacatata aaattggttt
     1561 agtgttaaaa gggtctgagg agcctattcg actgaatcca ttctacatca atgtattgct
     1621 aggaatttct gaaacgtgta accagcatgg ctatggtaca caaacgacag tctcaaataa
     1681 tatgaatgat ttaatggatg aagtttataa aatgattaaa caacgaatgg ttgatgcgtt
     1741 tatactgctt tattcaaaag aaaatgatcc aattaaacaa atgttaattg atgaaagcat
     1801 gccatttatt gtgattggta agcctacatc ggatatagat catcaattta cacacataga
     1861 taatgataat atattagctt ctgaaaattt gacacgacat gttattgaac aaggtgtaga
     1921 tgaattaata tttattacag aaaaaggaaa ttttgaagtt tcaaaagata gaattcaagg
     1981 atttgaaacg gttgcatcac aatttaatct ggattatcaa attattgaga ctagtaatga
     2041 aagagaagtt attttaaatt acatgcaaaa tctacatacg cgtttgaagg acccaaatat
     2101 taaacaggca atcatttcgt tagatgctat gttacattta gcgattttaa gtgtcctata
     2161 tgaacttaat attgaaattc cgaaagatgt aatgacagca acgttcaatg attcttattt
     2221 aaccgaaatt gcgtcaccac ctcaaacctg tattgacatc aagcctcgaa tgttaggtca
     2281 acaggctggt tcagcaattt taaatatatt gaaaaacaaa gcacaggatg ttattgaact
     2341 cgtcattata gatacagaat taaaaataag aaaatcaaca cagcgatagc aaaggagttt
     2401 taaaatgaat aagcaatggt ggaaagaagc agtagcatat caagtatatc caagaagttt
     2461 taatgatagt aatcacgatg gtattgggga tttacctgga atgattgata aattggacta
     2521 cttaaaagat ttaggtatcg atgtcatttg gctcagtcca atgtttaaat cacctaatga
     2581 tgacaatggt tatgatatta gtgactatca agaaattatg gatgaatttg gaacgatgga
     2641 agactttgat tgtttattaa aaggtattca tgatagaggc atgaagctta ttttagattt
     2701 agttgtaaat catacatctg atgaacatcc ttggtttata gaatctaaat ctagtaaaga
     2761 caatcccaaa cgtgattggt atatttggca agatccaaag caagatggct ctgaacctaa
     2821 caactgggaa agtatcttta atggatctac atgggaatat gatgctaata ctgagcaata
     2881 ttatttccat ttattcagta aaaaacaacc tgatttgaat tggggtaatc cagaagttag
     2941 agatgctgta tttgaaatga tgaactggtg gtttgataaa ggcattgatg gatttagagt
     3001 agatgcaatt acgcatatta agaagacgtt tgaagcgggt gacttacctg tacctgaggg
     3061 taaaacatat gccccagcat ttgatgtaga tatgaatcag ccaggtatac aaacttggtt
     3121 acaagagatg aaagatcgct cattaagtaa gtatgacatt atgactgtcg gtgaagcgaa
     3181 tggtgtaagc cctgatgatg ctgatgactg ggtcggggaa gaaaatggta aatttaatat
     3241 gatattccaa tttgaacatt tgggactgtg gaatagtggt gattctcact ttgatgtaaa
     3301 ttcgtataaa tctgtattaa atagatggca aaaacaactt gaaaataaag gttggaatgc
     3361 gttgtttatt gaaaatcacg accaaccacg acgtgtatcg acgtggggtg acgatgacaa
     3421 gtattggtat gaatcagcaa caagtcatgc agctgtttat ttcttgcaac aaggtacgcc
     3481 attcatttat caaggtcaag aaattggtat gacgaattat ccatttgaaa gtattgaaac
     3541 gtttaacgat gttgctgtaa aaaatgacta tcaaatagtg aaagctcaag gtggagatgt
     3601 agacgcttta cttgcgaaat ataaagatga gaaccgagat aattctcgca caccaatgca
     3661 atgggatgat acgttaaatg gaggatttac aaatggtgaa ccgtggttcc cagtgaatct
     3721 gaattataaa actatcaatg ttgcacaaca attagaagat gagcattcag tattacaatt
     3781 ttataaagat ttaattcaat taagaaagtc taatgatgta tacgtatatg gtcaatttga
     3841 tttagtagat gctgaaaatt cacaagtttt tgcgtacacg agaacattaa atgaaaagca
     3901 agttcttatt gtaggtaatc ttactaacca cgaagcagaa ttaactgtac catttgattt
     3961 aagccatgga gaagtaaagc tatttaatta tgatgccaaa gttaatttaa aacagttacg
     4021 tccatatgaa gcatgtgtta tcgaactaaa ttaacatatt aatatgcccc ctaatagaat
     4081 ctaaggccat ttgtagacct atagatatat tagggggctt ctttgttttt aatttttaat
     4141 cttcgtcata aggactctgt ttaacaggaa tccaaagctg aattttagtg aatgggtcat
     4201 taaatgaaat atcgagaggg tacacttcaa catataaatc atttcgttca tatggcaatg
     4261 ttaactgcaa acttgattca atatagtacc aagcttcatt agttgcataa tcaatttcac
     4321 cttgtaaatt gaatttcgca caatgtttgc caggtaaaaa tcggctttct aagtgtgcag
     4381 gataacgttc acttggtaca cctacaaata tttctaaacc attttcaaga ggacaactaa
     4441 taacaaatag ttcaaatgga ctaacgtcat tatatcgtcg taactcttta attttaccat
     4501 caatgagcaa gtcttctaaa aaatccggaa cattaaaagg atgtgacaaa tacttagtgt
     4561 ctataaatcg tgcatatcca accaatgaaa tatcatctgt ctcttctaat ctgtatggat
     4621 agggtgctct ctcagtagtt gataatttga tatataatcg ctcttgaatt tgtaattcat
     4681 cttttttagt agaagcttga ataggtgata cgccgtgaaa atcactaaaa tcatttgcaa
     4741 aatcatttga atttgcatag tgatattttt tagcgatatc tacaagtcgt gtagcaccat
     4801 taatcacatc atttgcagcg agtgtcattt tacgcgcgcg tgcataagct tctggagata
     4861 agccgacaat cattttaaat gattgatcaa gatggtatgg cgaaagacca acgtaatcac
     4921 ttaattcttg caaattgaaa ggctctaata aacgatcttc aatataaaca attgcctgtt
     4981 gtatttgctt gataacgtcc aaaactttca ctccaattaa ttcaatcatt tatttttatt
     5041 ctacattatt tctataaatt atacacccat ttgttcaatg attattaaaa tagttttggg
     5101 cattgtaaaa tataatttca taatatagtc tagaaaaaaa gcgaatgata gaacaattga
     5161 tttacttgat tcgtaatcaa tccttgtcat tcgctcattt atttttgttt aacatgtgcg
     5221 ttttaattca attattgaat atcgtcccac caatggttac catcacgagc aagtagtaaa
     5281 tcactttcta atggaccatt agtacctgat tcatagttag ggaattctgg atcaaccata
     5341 ttccattcat cttgaattgc atcaacaaat ttccatgttg attttaattc ttcccagtgc
     5401 gtgaagttag tggcatcacc tttaagacaa tcgaataata gattttcata tgcatctaca
     5461 gtattcattt tatcttgagc gctcattgaa taagacaatt gaacaggttc agtttcgata
     5521 ccttgtgtat ttttcttagc atttagatgt aaagatacac cttcattagg ttggatattg
     5581 attactaata ggtttgaatc taatagttta tcagtttcat agtataagtt cattggtact
     5641 tctttaaatt caacgacaac ttgaattgtt ttagatttca tacgtttacc agtacgaata
     5701 tagaatggta caccagccca tctaaagtta tcaattgtta atttacctga aacaaaggta
     5761 ggtgtgttag agtcatctgc aacgcgatct tcatcacggt atgctttaac ttgtttacca
     5821 tcgatatagc cttcgccata ttgaccacga acaaagttct ttttaacatc ttcagattgg
     5881 aaatgacgca gtgatttaag tacttttact ttctcagcac ggatatcttc actgtttaaa
     5941 ctaataggtg cttccatagc taataatgca accatttgta acatgtggtt ttgcaccata
     6001 tcttttagcg cgccacttga ttcataataa ccaccacgat cttcaacacc tagtatttca
     6061 gaagatgtaa cttggatgtt tgaaatatat ttgttattcc ataatggttc aaacatcgca
     6121 ttcgcaaaac gtaatacctc gatattttga accatgtctt ttcctaaata gtggtcaata
     6181 cgataaattt cttcttcttt aaatgattta cgaatttgat tgtttaatgc ttcggctgat
     6241 tttaaatcac taccgaatgg tttttcgata acaaggcgtt taaatccttt tgtatcagta
     6301 agaccagaag attttagata atcagaaata acgccaaaga attgtggtgc cattgctaaa
     6361 tagaatagtc gattaccttt taattcaaat tggctatcta attcattact aaaatctagt
     6421 aattcttgat agctttcttc attactaaca tcatgtctat gatagaagac atgttccata
     6481 aacgcgtcaa ttttgtttgt atcttttacg tgcttttgaa ttgatgattt tacttgatta
     6541 cggaaatcat cattagtaat gtcacgacgt ccaataccga tgatggcaat atgttcatct
     6601 aaattgtctt gttggtagag atggaatatt gatggaaaca acttacgatg gcttaagtca
     6661 ccagttgcac caaagattgt gattaaacaa gggatgtgtt tgtttttagt actcaagatt
     6721 aaaacctcaa ttcttaatta gatatatgat ttattattat aagataatcc attcagcagg
     6781 tcattcaata tgctcgacag tatgcagtgt cttaaatgaa atatcgattc atgtatcatg
     6841 tttaatgtga tacttattaa tgataagtat aacgtaattg tcaaaattta tatagttatg
     6901 tctacgttaa agttagaaaa attaactagc aaagacgaat ttttaacaga ttttgattca
     6961 agtataaatt aaaactaaat tgatacaaat tttatgataa aatgaattga agaaaaggag
     7021 gggcatatat ggaagttaca ttttttggaa cgagtgcagg tttgcctaca aaagagagaa
     7081 atacacaagc aatcgcctta aatttagaac catattccaa ttccatatgg cttttcgacg
     7141 ttggtgaagg tacacagcac caaattttac atcatgcaat caaattagga aaagtgacac
     7201 atatatttat tactcatatg catggcgatc atatttttgg tttgccagga ttactttcta
     7261 gtcgttcttt tcagggcggt gaacagaagc cgcttacatt ggttggacca aaaggaatta
     7321 aagcatatgt ggaaatgtct atgaatttat cagagtcaca tttaaactat ccaattacat
     7381 atattgaaat cgatgatcat ctgacatatc atcatgatgg ttttactgta gaggcgcatt
     7441 tacttaatca tggtattcca tcatatggat atagagtaat ggcacctgaa actacaggta
     7501 caatcaatgt tgaagcgtta aaaaacattg gtcttgaacc aggtcctaaa tatcaagaag
     7561 taaaatctca tgatacattt gaacataatg gtcaagttta tcaatcaaaa gattttagag
     7621 gtgagtccaa acagggtcca gttgtagcaa tttttggtga tacaaaacca tgttctaatg
     7681 aacgtgtgat ttctagagat gctgatgtga tggtacatga agcgacctat atagatggtg
     7741 aaaagcattt agccaacaat tatcatcata gccatattga agacgtattt gcattgataa
     7801 aagaagccaa tgttaaacgt acgttgatta cacatttgag caatcgctat aatacagaag
     7861 atataaatga aatttatcaa atattgatac aaaatgaaga tacacctaat tttaattttg
     7921 taaaagattt tgatagtttt aaagtctaat gacaaagtaa taaacaatgc ttgccaaata
     7981 gtaataattt ataaaaataa cccgtccaac tatgtatgtg atgatgcata catgtgttgg
     8041 cgggtttgtt tttattggtc ttctacatta gaaagttcaa tactacggtc gacggcagca
     8101 tttagacaat cttcgaaaat agatactaaa tcatattgtg acaatgtatc aaggccagct
     8161 tgtgtcgtac cacctttaga ggtaatattt tttcttaatt gagccatgct caaatctgaa
     8221 cgttcaatca tcttacttgt acctataata aggttgcgta tagattcttc aacttgttct
     8281 ttttctagac caagtttcgt acaagcttta acgtattgct cgaatacatg atataaaaat
     8341 gctgggccgc ttccggtgat agctgttact tgatgtaaat gatcctctga tacttcaatt
     8401 acagaaccaa atgctttaac taaatcgtta atttcatctt tagatttagg gtcaaagttg
     8461 tttgaaaaac taatgccagt aactgagtgt ccaacttgcg catttgtgtt tggcataatt
     8521 ctagcaactg gattttggca ttctaattgt tgtttaatat aatcaatcgg aatacctgcc
     8581 ataattgaaa tgaagcaatt gtcttttgtg atatgtggtt tgatgcgtgt tgctagagca
     8641 tcaaagtcat gcggtttggt acctaaaaaa acataatctg catcttttaa taatgtcgca
     8701 tcatcataac tatagttaac acctagtttt tcagcgaatg cttttaaagc ttgttcatta
     8761 gatttatttg ttaaatatat atcattggca tctaagttgc ttgagttaat aattcctgta
     8821 aatatagctt gtgccatatt accagctcca taaaatacga gtttcattaa cgtcacatcc
     8881 ctttagtttt tgatttgtat gttatatttt cttattattg tatatatatt aggaaagata
     8941 atcaacttgt ctcaattata cagagacgat aaattgcatt ctatataatt gaaaaggaag
     9001 gttaattgat gaaaggacaa cattttatag tgactggtgg tacgagtggt ttaggtttat
     9061 cgattgtccg aaagttacta gaaaataaag tgcatgtaac gttgttggtg cgtgatgttg
     9121 ataaagcaac tagaatcttt gaacaagaat tggggaaaac tataaatgtt attccttgtg
     9181 acttaaatga tttgaagtcg atacaggcac tgcaatttga agacaataca tcattcgatg
     9241 gattcattta tagtgcaggt ttaggttatt tcaaatctat ttcagaccat agtttttcag
     9301 aaatgattga aacgtatcag cttaatttaa ttagttttaa tgttttatat actgtgttga
     9361 gaccatattt aacatctaac gcacatattg taggtatttc aagtcaagct gcattttcca
     9421 cacaagcaaa tgctgcacat tatggggcat cgaaagcagg gttttatgca ttaatgaatg
     9481 ctttaagact agagtcacca aacttacata tcatgacagt taatgttggc ccgattgata
     9541 caccttttca tcaaaaagcc gacccatcaa tgaagtatgc taaaaagatg ggggaaatta
     9601 tgcttgatgc taatcagctt gcagaagata tcatttatgg cattaaaaca aaacaattgg
     9661 aaattaatcg gcctaaatgg atgcatcatg cgttgaaaat gtatcaaatt gcgcctagat
     9721 tttttgaacg atgtttccct aaattattta aaaataaagc ataaataggt gggatggcaa
     9781 tgaactgtca atcggagagt gtggtaagac tttgtgtacg atatgcggaa caactatcag
     9841 tatttgaaga atttacagtg ctggatattt taggcgatat ttctgtagac caaatttcag
     9901 acggtacatt atattatact tgtgaaaaat tcaaactact tgtgttgcaa ggaaatgtgc
     9961 tgggtgtaca aataataact aataatgatg aatcaacatg cgaagtgaaa tatagaaaga
    10021 tgttttaata aaattattaa gagatgaagt ggcagaagtt tgcttaaggc aatgccgcaa
    10081 ggcgaaatta ctaaatactg atgcgtgagg acaacgcttt aatttctatc ataagtgagt
    10141 aatcataatc ataaattata gcgaattgta cataaacaac agcagtaagg catttttata
    10201 atgaaacgcc tcatgctgct gtttttatta ctgaaaatga cttctacact aaatgattgg
    10261 tatattccaa atcctttaca cgagctcttg ctgttttaat ctgatctaaa ccaactttag
    10321 tatgatagtt ttcaatattt tcttttaatt ggtcgacgct acttgcacct acaatgatgg
    10381 aaccaagcac gtcatgtgat gttaaataac taaatgtcaa tgcagataaa ttactttcaa
    10441 tttctttaat agaggctatt gtttcaccca attcatcatg agaataatca aaaataccat
    10501 ctttaaattt attgtcgagc acattaacac tgtttgaagt taataatcct ttagacacag
    10561 gtcctcttgc caatacttta acatcattgt tgtgaattgc atctaataat gattctggac
    10621 gattatcaat caaattgaat tgagacatta tcgtttcgat ttgactatgt tttaaataat
    10681 aatcaattac atttgggcga atagaagaaa taccgtaagc acgtataatt ccttcttgtt
    10741 tcaattcatc aaatgcgctt attgtttcgt ctaatgggtc atcaatggtt ccgccatgaa
    10801 gttgatataa atcgatatga tcgataccta aacgctttag tgaaccttta actgcctctt
    10861 taatatagga tttactcgga tcccatgttg tactgccatc ttttgttaaa cgattgccta
    10921 cttttgtacc aatgaaaata tcatcgcgtt gttgatattt aagaagtgcc ttaccaacaa
    10981 ctgattcatt tataccttta tcgtacatgt ctgctgtatc aaaataagtg ataccatttt
    11041 caacagcaca atctataatt tgttcggctt tttttaaatc tgtacctaaa ctcatgcaac
    11101 ctagacctaa ttcagataat gcaataccac tttttaatat atttttttgc atgtctgttc
    11161 tcctttcgat acactttagc taacaagcat agtgtatcaa tatataatat gaatttaaag
    11221 gaacgtgatt ataatggatt taaatgaaaa aacaattgat cgaacagtta tttataatgg
    11281 caaaattgta gatgtagaaa ttcatacagt gacattacca aacggtgaaa cgtcaacaag
    11341 agaattagtt tatcataatg gtgcagttgc tgtttgtgca ttgacaccta aaaaagaagt
    11401 cgtattagtg aaacagtatc gtaaaccagt agaaaaacca ttgctagaaa ttccagctgg
    11461 taaattagaa gatgatgaaa atagagtcga agcggctaag cgtgaattag aagaagaaac
    11521 aggatatatt gccaaagagt taacacatgt tgtagatatg tatggttcac caggtttttg
    11581 cgatgaacaa ttatcaattt attttactga taatttagaa gaaggtacag ttcacttaga
    11641 tgaagatgaa tttgtcgaag tcattaaagt tccgattgag aatgttaaat cgatgctaat
    11701 gaataaagaa atagaagatg cgaagactat cattgcattg caacacctat tattaaatta
    11761 taatcattct aaataacaaa tgagaaaagc ttgcatttta ttgagaaaac tgttagtttt
    11821 aattgtgaag tttgaaataa tttgtaatga ttttaattat tagtagggga gtggacatcg
    11881 ttggaagaac gattaaatcg cgttaagcaa caattacaac aatcatcata taagctaacg
    11941 ccacaacgcg aagctactgt tagagttcta attgaaaatg aaaaagatca tctaagtgct
    12001 gaagacgtat atctgaaagt aaaagacaaa gcgcctgaaa ttggcttggc gacagtatac
    12061 agaacgttag agttgttagc tgaactaaaa gttgtcgaca aaattaactt tggtgatggc
    12121 gtcgctcgtt ttgatttaag aaaagaaggc gcaaaacatt tccaccatca tttagtatgt
    12181 atggaatgtg gtcgtgtaga tgaaatcgat gaagatttgt taccagaagt tgaaaatcga
    12241 gttgaaaatg agttcaattt taaaatttta gatcatcgtt taactttcca tggtgtgtgt
    12301 gaaacatgcc aagctaaagg taaaggatag taaattgcgt aggttaaatt aaccttcgct
    12361 ttttttagag gtgtggttat ggaaacaatt attgaagaat atttgcgttt tatacaaatt
    12421 gaaaaaggac taagttctaa cacaattggt gcttatagac gtgatttgaa aaagtatcaa
    12481 gattatatga ctgaacatca tatctcgcat attgatttta tagatcgaca attaattcaa
    12541 gagtgtttgg ggcatttaat agaccaaggg caatctgcta aatctattgc gcgatttatt
    12601 tcaacaatcc gtagttttca tcaatttgct ataagagaaa aatatgcggc gaaagatcca
    12661 acggtattat tagattcacc aaaatatgac aaaaaattgc ctgacgtttt aaatgttgac
    12721 gaagtattgg ctttattaga aacgccagat ttaaataaaa ttaatggata tcgtgatcgt
    12781 acgatgttag aacttctgta cgcaacggga atgcgtgtat ctgaattgat acatttagag
    12841 ttagaaaacg tgaacttaat aatgggattt gtacgcgtat ttggtaaagg cgataaagaa
    12901 agaattgtac cattaggcga cgcagtcatt gagtacttaa ctacttatat tgaaacgatt
    12961 agaccgcaac ttttaaaaaa gactgttact gaagtcttat ttttaaatat gcatggtaaa
    13021 cctttatcac gacaagcaat atggaaaatg attaaacaaa atggcgtaaa ggcaaacatt
    13081 aaaaagacgt taacgccaca tacgttacgc cactcttttg cgacacattt attggaaaat
    13141 ggcgcagatt taagagcagt gcaagagatg ttaggtcact ctgacatatc tactacccaa
    13201 ctctatacac atgtttcgaa atctcaaatt agaaaaatgt ataaccaatt tcatcctaga
    13261 gcataaagtg aaccataact taaaagtcac aatacacatg actaaaaatg tctgtgctat
    13321 tgtggctttt ttaaattggt tgattaatta cgtctatgtt ttcttaattg aatcgcttct
    13381 tcttttgctg caatcacttc tgaacgatca cgccgcatgt gatggtctac aataaaagga
    13441 tctgttgctg tttcctgatt ataatcatag tctggatagt tggccttgat gatgcgttca
    13501 aagactggag ttattggtaa tataacagat gaaaaaggct ttgctgcatt caattttgca
    13561 atctgttgct caattaacaa ttgataatca tttaaattaa ggtataacgc atctctatct
    13621 ttagcatttt gtattatttc tttagattta ttaaaagact tataggcgcc ttttaaatta
    13681 ttgcggcgat aatggtaaca agcagttgca aacaagatta aactaacaac tgcatcttgc
    13741 ttactgtagt tattttcagc tttccatgca tcttctaaaa tgtcatgaca taggaaataa
    13801 tgttgcttag tatgaaattg ataatagaaa tttatcagtg cctgttgcat tttgttatca
    13861 ccccaattta aaagtaagtt attttcatgc tataatattt tagagaatta tgcacatatg
    13921 acgcaatacg aggtagatat tatgtatgaa gttaaattag atgctttcaa tggaccatta
    13981 gatttattgc tgcatcttat ccaaaaattt gaaatagata tttatgatat tcctatgcaa
    14041 gcattaacag agcagtatat gcagtacgtt catgcaatga aacagcttga aattaatatt
    14101 gcaagtgaat acctagtatt agcgtcagaa ctcttaatga ttaaaagtaa gatgctatta
    14161 ccacaatcaa catcagatat ggatgttgat gatgacccac gggaagattt agttgggcgt
    14221 ttaatagaat atcaaaatta taaagaatat actgctattt taaatgacat gaaagaagaa
    14281 agagattttt attttacaaa aagaccgaca gatttatctc atttggaaac agatgaatct
    14341 tgggatccaa atcatacgat tgatttaact gaattaattg tagcttatca aagagttaaa
    14401 aatagagttg agttaaatac acctaaatct gttgaaatcc gaaaagagac atttaccatt
    14461 caacaagcta cagaacaagt gacatcgaga ttgaaagata aagatcattt taacttcttt
    14521 agtctgttta cgttttctga gccaattgaa caagtagtca ctcacttttt agctatttta
    14581 gagatgtcaa aagcaggaat aattaatatt gagcaacaac gtaattttga agatattaac
    14641 attattagag gagtgaacta ccattttgga taatcatggt atattagagt cgcttttatt
    14701 tacagctggc gatgaaggtt tagatgaaaa acaactatta gaaatattag atatgtcgaa
    14761 agaccaactc gttgaattaa ttgaaaatta ttcatcacat ggattaatga tacaacgatt
    14821 tggaacgacg tatgttttaa cgactaaaaa agaagcggca acgtatattg aacaattaat
    14881 tgaacaaaag tcacaaatga aattatcaca agcagcaatg gaagtactat caattattgc
    14941 ttataaccag ccattatcaa gaagtgatat tgaattaatt cgtagtatca attcagatgg
    15001 tgcagttaag acattgattg ccaaaggact agttgaggct aaagtggtta atgaacagcg
    15061 tagccaacag ttaattacta ctgatttatt tttaaatgta tttggtattt ccaatataga
    15121 agatttgccg acaactgaag aagacgatga agaaatggat gcttttttca gtaatctagt
    15181 caatcaaaaa ggagaaaata atgactaaag aattagaacg attacaaaaa cgtatagcta
    15241 atagcggtta tacttcaaga cgtaaggcag aaactttaat ttctgaagga aaagtgaaag
    15301 tgaatggtac gactgtcact gaattaggaa caaaagtgaa gccatcagat acgattgaag
    15361 ttgaaggtat taaaatagaa ctagaagaca aaatttacat tttattccat aaaccaactc
    15421 aagtgataac aagtgtatct gatgatagag gacgtacagt ggttacagat tactttaaag
    15481 atatagaagc acgtatttat cctgttggtc gtttagacta tgatacttct ggattgttat
    15541 tacttacaaa tgatggtgaa tttactaatt taatgacaca tccaagatat caaattaaga
    15601 agaaatatgt agcaaaatta aaaggttact taatgagaga agaagtgaaa gccctagaaa
    15661 aaggtattga attagaagat ggtatgacgc aaccggctga agtgaaagtt aagaaacaag
    15721 ataaagataa aaacacaaca ttggttgaaa ttacaattac agaaggtcgt aatcgccaag
    15781 tcagaagaat gttcgaacat tttggtcatc aagtgacgaa actgtctcgt attgaatatg
    15841 ggcctttaaa tgttgtcggt ttgaatgctg gtgaaggacg tgtattgacg cctcatgaag
    15901 taaaagtgat gcgtcattta gcagaacatg ggaaataatt aaataaaata tgtatttatc
    15961 acaaagtttg agaatttttt cacaaaattt taggttttta ataactgtat gcgctttcct
    16021 gtgatataat acttttgtaa agaaaagcat gtgtgggagg tatgacctgt atgtcgaacg
    16081 aaatacttat cgtagatgat gaggatagaa tcagaagatt acttaaaatg tatttagaaa
    16141 gagaatcttt tgaaatccat gaagcaagta atggccaaga ggcttatgaa cttgcaatgg
    16201 agaataatta tgcttgcata ctactagatt taatgttgcc tgaaatggat ggtatccagg
    16261 tggcaactaa attgcgtgaa cataaacaaa caccgattat tatgttgact gctaaaggtg
    16321 aagaaacaaa ccgtgttgaa ggttttgaat ctggtgcaga tgattatatc gtcaaaccat
    16381 tttcaccaag agaagtagtc ttaagagtta aagcacttct aagaagaacg caatctacaa
    16441 ctgtagaaca aagcgaacct cacgcacgtg atgtgattga atttaaacat ttagaaatag
    16501 ataatgatgc acatcgcgta cttgctgata atcaagaagt taatttgact cctaaagagt
    16561 acgaattatt aatatattta gctaaaacac caaataaagt atttgaccgt gaacaattat
    16621 taaaagaagt ttggcattat gaattctatg gtgatttaag aacagttgat actcatgtta
    16681 aacgacttag agaaaagtta aatcgtgtgt ctagcgaagc tgcgcatatg attcaaacag
    16741 tctggggcgt tgggtataaa tttgaggtta aatctaatga tgagccggct aaatagtgtc
    16801 gtaattaaac tgtggttaac tattatttta atagtgacga cagttttaat tttattaagt
    16861 attgctttaa ttacctttat gcaatactat ttcacacaag aaaccgaaaa tgccataaga
    16921 gaagatgcta gacgtataag ttcactggtc gaacaatcac ataataaaga agaagcaata
    16981 aaatatagtc aaacattaat tgaaaatcct ggtgggttga tgattataaa taataaacat
    17041 cgtcaatcaa cggcttcact ttctaatatt aaaaagcaaa tgttgaatga agtagtcaac
    17101 aacgaccatt ttgacgatgt gtttgataaa ggtaaatctg ttactcgaaa tgtaacgatt
    17161 aaagaaaagg gttcatctca aacatatatt ttgttaggct atccaacaaa agcacagaag
    17221 aatagtcata gcaaatatag tggagtcttt atatataaag acttgaaatc aatcgaagat
    17281 acaaataatg ctattacgat tatcactata attacggctg ttattttctt aacaattaca
    17341 acagtctttg cgtttttctt atcgtcaaga attacaaaac ctttaagacg tttaagagac
    17401 caagctacac gtgtatctga aggggattac tcttataaac cttctgtcac aacgaaagat
    17461 gaaattggtc aattatcgca ggcatttaat cagatgagta cagaaatcga agagcatgtc
    17521 gacgcattat ccacatctaa aaatattaga gacagcttaa ttaactctat ggtagaaggt
    17581 gtcctaggta ttaatgagag tcgacaaatt atcttatcta ataagatggc gaatgatatt
    17641 atggacaata ttgatgaaga tgctaaagct ttcttattaa gacaaattga agatactttt
    17701 aaatcaaaac aaacggaaat gcgtgattta gaaatgaatg cacgatcctt tgttgtgacc
    17761 acaagctata tcgataagat tgaacaggga ggtaaaagtg gtgttgttgt gacagttcgt
    17821 gatatgacta atgagcacaa tctagatcaa atgaagaaag atttcattgc taatgtatca
    17881 catgaattac gaacacctat atcattacta caaggttata ctgaatcaat tgtagatggt
    17941 attgttacag aaccggatga aataaaagaa tcgctcgccg ttgtccttga tgaatcgaaa
    18001 cgtttaaatc gattagttaa tgaattgtta aatgtcgcac gcatggatgc tgaagggtta
    18061 tccgtaaata aagaagttca gcctattgca gcgttactag ataagatgaa aattaagtat
    18121 cgccaacaag ctgatgattt aggtctaaat atgactttta attattgtaa gaagcgtgtt
    18181 tggagttatg atatggatcg catggaccaa gtactaacga acttaattga taatgcatca
    18241 cgttatacga aacctggaga tgaaattgca attacttgtg atgaaaatga aagcgaagat
    18301 attttataca ttaaagatac aggcacaggc attgcaccag aacatttaca acaagtattt
    18361 gatcgttttt ataaagttga tgcagcgaga acgcgaggta aacaaggtac cggtttaggt
    18421 ttgttcattt gtaaaatgat tatcgaagag catggtggtt ccattgatgt taaaagcgaa
    18481 ttaggaaaag gcacaacatt tattattaaa ctaccaaaac cagaataaaa ctgaatatag
    18541 ttatttaaga acgcatgtta ttgattagag actctaattt atagcatgcg ttttttgatt
    18601 gatgtcgaaa gttttgtaag tggattagga
//
LOCUS       DANBWH010000029        18455 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.029, whole genome shotgun sequence.
ACCESSION   DANBWH010000029 DANBWH010000000
VERSION     DANBWH010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18455
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.029"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            41..1306
                     /gene="murA"
                     /locus_tag="PPY92_000703"
     CDS             41..1306
                     /gene="murA"
                     /locus_tag="PPY92_000703"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDJ2624726.1"
                     /translation="MDKIVIKGGNKLTGEVKVEGAKNAVLPILTASLLASDKPSKLVN
                     VPALSDVETINNVLTTLNADVTYKKDENAVVVDATKTLNEEAPYEYVSKMRASILVMG
                     PLLARLGHAIVALPGGCAIGSRPIEQHIKGFEALGAEIHLENGNIYANAKDGLKGTSI
                     HLDFPSVGATQNIIMAASLAKGKTLIENAAKEPEIVDLANYINEMGGRITGAGTDTIT
                     INGVESLHGVEHAIIPDRIEAGTLLIAGAITRGDIFVRGAIKEHMASLVYKLEEMGVE
                     LDYQEDGIRVRAEGELQPVDIKTLPHPGFPTDMQSQMMALLLTANGHKVVTETVFENR
                     FMHVAEFKRMNANINVEGRSAKLEGKSQLQGAQVKATDLRAAAALILAGLVADGKTSV
                     TELTHLDRGYVDLHGKLKQLGADIERIND"
     gene            1340..1780
                     /gene="fabZ"
                     /locus_tag="PPY92_000704"
     CDS             1340..1780
                     /gene="fabZ"
                     /locus_tag="PPY92_000704"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDJ2624727.1"
                     /translation="METIFDYNQIKQIIPHRQPFLLIDKVVEYEEGQRCVAIKQVSGN
                     EPFFQGHFPEYAVMPGVLITEALAQTGAVAILNSEENKGKIALFAGIDKCRFKRQVVP
                     GDTLTLEVEITKIKGPIGKGNAKATVDGQLACSCELTFAIQDVK"
     gene            complement(1839..2279)
                     /locus_tag="PPY92_000705"
     CDS             complement(1839..2279)
                     /locus_tag="PPY92_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwpF-like family protein"
                     /protein_id="HDJ2624728.1"
                     /translation="MKTFKAVRFQIVNEHGRIIEYELEDGVIINKEESGTGWLLEIVI
                     SNEHYETFKEYQDNEQLLDIRVVITRPANDPALFESTVKSIKNFKTTMSIVFECHIYT
                     LRQQYAESLLEQLIDDGLSGEELKKSFNRMMQSKPKLKDEKLEE"
     gene            2473..2868
                     /locus_tag="PPY92_000706"
     CDS             2473..2868
                     /locus_tag="PPY92_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDJ2624729.1"
                     /translation="MLNKIVIVGRLTKDAQIFEKEDRKIATFCVATHRNYKDENGEIV
                     CDYLFCKAFGKLASNIEKYTNQGTLVGITGQMRSRKYDKDGQTHFVTELYVETIKFMS
                     PKSQNNEILSDSILDIDSQNIDNHDLLEI"
     gene            3257..3952
                     /gene="sceD"
                     /locus_tag="PPY92_000707"
     CDS             3257..3952
                     /gene="sceD"
                     /locus_tag="PPY92_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase SceD"
                     /protein_id="HDJ2624730.1"
                     /translation="MKKTLLASSLAVGLGIVAGNAGHEAHASEADLNKASLAQMAQSN
                     DQTLNQKPIEAGAYNYTFDYEGFTYHFESDGTHFAWNYHATGANGANMSAQAPATNNV
                     EPSAVQANQVQSQEVEAPQNAQTQQPQASTSNNSQVTATPTESKASEGSSVNVNAHLK
                     QIAQRESGGNIHAVNPTSGAAGKYQFLQSTWDSVAPAKYKGVSPANAPESVQDAAAVK
                     LYNTGGAGHWVTA"
     regulatory      4157..4259
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4339..5028
                     /gene="tenA"
                     /locus_tag="PPY92_000708"
     CDS             4339..5028
                     /gene="tenA"
                     /locus_tag="PPY92_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HDJ2624731.1"
                     /translation="MEFSQKLYQAAKPIINDIYEDDFIQKMLSGDIGADALRHYLKAD
                     AAYLKEFTNLYALLIPKMNSMNDVKFLVEQIEFMVEGEVLAHDILAQIVGESYEEIIK
                     TKVWPPSGDHYIKHMYFQAHSRENAIYTIAAMAPCPYIYAELAKRSQSDHKLNREKDT
                     AKWFDFYSTEMDDIINVFEALMNKLAESMSDKELEQVKQVFLESCIHERRFFNMAMTL
                     EQWEFGGKVND"
     gene            5021..5851
                     /gene="thiD"
                     /locus_tag="PPY92_000709"
     CDS             5021..5851
                     /gene="thiD"
                     /locus_tag="PPY92_000709"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDJ2624732.1"
                     /translation="MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVA
                     QNTLGVQHIHNLNHQWVDEQLDSVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIP
                     YVIDPVMLAKSGDSLMDNDTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQ
                     AGRIFINEIGSKGIIIKGGHSNDTDIAKDYLFTNEGVQTFENERFKTKHTHGTGCTFS
                     AVITAELAKGRPLFEAVHKAKKFISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK
                     "
     gene            5835..6626
                     /gene="thiM"
                     /locus_tag="PPY92_000710"
     CDS             5835..6626
                     /gene="thiM"
                     /locus_tag="PPY92_000710"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDJ2624733.1"
                     /translation="MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPE
                     EAEEFYKVAQALLINIGTLTAQNEQDIIAIAQTANEAGLPIVFDPVAVGASTYRKQFC
                     KLLLKSAKVSVIKGNASEILALIDDTATMKGTDSDANLDAVAIAKKAYAIYKTAIVIT
                     GKEDVIVQDNKAIVLANGSPLLARVTGAGCLLGGVIAGFLFRETEPDIEALIEAVSVF
                     NIAAEVAAENENCGGPGTFSPLLLDTLYHLNETTYQQRIRIQEVE"
     gene            6628..7269
                     /locus_tag="PPY92_000711"
     CDS             6628..7269
                     /locus_tag="PPY92_000711"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDJ2624734.1"
                     /translation="MFNQSYLNVYFICGTSDVPSHRTIHEVLEAALKAGITLFQFREK
                     GESALKGNDKLVLAKELQHLCHQYDVPFIVNDDVSLAKEINADGIHVGQDDAKVKEIA
                     QYFTDKIIGLSISDLDEYAKSDLTHVDYIGVGPIYPTPSKHDAHIPVGPEMIATFKEM
                     NPQLPIVAIGGINTNNVAPIVEAGANGISVISAISKSENIEKTVNRFKDFFNN"
     gene            7355..8227
                     /gene="yidC"
                     /locus_tag="PPY92_000712"
     CDS             7355..8227
                     /gene="yidC"
                     /locus_tag="PPY92_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDJ2624735.1"
                     /translation="MKKKALLPLFLGIMVFLAGCDYSKPEKRSGFFYNTFVDPMKNVL
                     DWLGNNLLNDNYGLAIIILVLVIRIILLPFMLSNYKNSHMMRQKMKVAKPEVEKIQEK
                     VKRARTQEEKMAANQELMQVYKKYDMNPIKSMLGCLPMLIQLPIIMGLYFVLKDQLVD
                     GLFKYPHFLWFDLGRPDIWITIIAGVLYFIQAYVSSKTMPDEQRQMGYMMMVISPIMI
                     IWISLSSASALGLYWSVSAAFLVVQTHFANIYYEKVAKKEVQPFIEAYEREHNGGSNK
                     KGKNTQVVSKKKKK"
     gene            complement(9040..9687)
                     /locus_tag="PPY92_000713"
     CDS             complement(9040..9687)
                     /locus_tag="PPY92_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDJ2624736.1"
                     /translation="MQQSDVISAAKKYMESIHQNDYTGHDIAHVYRVTALAKSIAENE
                     GVNDTLVIELACLLHDTVDEKVVDANKQYVELKSFLSSLSLSTEDQEHILFIINNMSY
                     RNGKNDHVTLSLEGQIVRDADRLDAIGAIGVARTFQFAGHFGEPMWTEHMSLDKINDD
                     LVEQLPPSAIKHFFEKLLKLESLMHTDTAKMIAKERHDFMMMYLKQFFTEWNYHD"
     gene            complement(9715..11199)
                     /gene="cls"
                     /locus_tag="PPY92_000714"
     CDS             complement(9715..11199)
                     /gene="cls"
                     /locus_tag="PPY92_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDJ2624737.1"
                     /translation="MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANS
                     IWAWLLVLVFLPLFGFILYLLLGRQIQRDQIFKIDKEDKKGLELIVDEQLAALKNENF
                     SNSNYQIVKFKEMIQMLLYNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQ
                     YYIIQNDELGRTILNELGKKAEQGVEVKILYDDMGSRGLRKKGLRPFRNKGGHAEAFF
                     PSKLPLINLRMNNRNHRKIVVIDGQIGYVGGFNVGDEYLGKSKKFGYWRDTHLRIVGD
                     AVNALQLRFILDWNSQATRDHISYDDRYFPDVNSGGTIGVQIASSGPDEEWEQIKYGY
                     LKMISSAKKSIYIQSPYFIPDQAFLDSIKIAALGGVDVNIMIPNKPDHPFVFWATLKN
                     AASLLDAGVKVFHYDNGFLHSKTLVIDDEIASVGTANMDHRSFTLNFEVNAFIYDQQI
                     AKKLKQAFIDDLAVSSELTKARYAKRSLWIKFKEGISQLLSPIL"
     gene            11365..11658
                     /gene="csoR"
                     /locus_tag="PPY92_000715"
     CDS             11365..11658
                     /gene="csoR"
                     /locus_tag="PPY92_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-sensing transcriptional repressor CsoR"
                     /protein_id="HDJ2624738.1"
                     /translation="MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDV
                     LTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQEEAMEELLVTFQKLIKD"
     gene            11670..11879
                     /locus_tag="PPY92_000716"
     CDS             11670..11879
                     /locus_tag="PPY92_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDJ2624739.1"
                     /translation="MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVT
                     VSYETPANLNSIEKEIYDEGYKIVF"
     gene            12007..12144
                     /locus_tag="PPY92_000717"
     CDS             12007..12144
                     /locus_tag="PPY92_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624740.1"
                     /translation="MKRPEKIQNVVKLLSSLGVNIKKTKSRLDIINTLPASNKVSHEL
                     K"
     gene            complement(12408..13610)
                     /locus_tag="PPY92_000718"
     CDS             complement(12408..13610)
                     /locus_tag="PPY92_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HDJ2624741.1"
                     /translation="MNYSSRQQPDKHWLRKVDWVLVATIAVLAIFSVLLINSAMGGGQ
                     YSANFGIRQIFYYILGAIFAGIIMFISPKKIKHYTYLLYFLICLLLIGLLVIPESPIT
                     PIINGAKSWYTFGPISIQPSEFMKIILILALARVVSRHNQFTFNKSFQSDLLLFFKII
                     GVSLVPSILILLQNDLGTTLVLAAIIAGVMLVSGITWRILAPIFITGIVGAMTVILGI
                     LYAPALIENLLGVQLYQMGRINSWLDPYTYSSGDGYHLTESLKAIGSGQLLGKGYNHG
                     EVYIPENHTDFIFSVIGEELGFIGSVILILIFLFLIFHLIRLAAKIEDQFNKIFIVGF
                     VTLLVFHILQNIGMTIQLLPITGIPLPFISYGGSALWSMMTGIGIVLSIYYHEPKRYV
                     DLYHPKSN"
     gene            13928..14998
                     /locus_tag="PPY92_000719"
     CDS             13928..14998
                     /locus_tag="PPY92_000719"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDJ2624742.1"
                     /translation="MTKENICIVFGGKSAEHEVSILTAQNVLNAIDKDKYHVDIIYIT
                     NDGDWRKQNNITAEIKSTDELHLENGEALEISQLLKESSSGQPYDAVFPLLHGPNGED
                     GTIQGLFEVLDVPYVGNGVLSAASSMDKLVMKQLFEHRGLPQLPYISFLRSEYEKYEH
                     NILKLVNDKLNYPVFVKPANLGSSVGISKCNNEAELKEGIKEAFQFDRKLVIEQGVNA
                     REIEVAVLGNDYPEATWPGEVVKDVAFYDYKSKYKDGKVQLQIPADLDEDVQLTLRNM
                     ALEAFKATDCSGLVRADFFVTEDNQIYINETNAMPGFTAFSMYPKLWENMGLSYPELI
                     TKLIELAKERHQDKQKNKYKID"
     gene            15013..16371
                     /locus_tag="PPY92_000720"
     CDS             15013..16371
                     /locus_tag="PPY92_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDJ2624743.1"
                     /translation="MINVTLKQIQSWIPCEIEDQFLNQEINGVTIDSRAISKNMLFIP
                     FKGENVDGHRFVSKALQDGAGAAFYQRGTPIDENVSGPIIWVEDTLTALQQLAQAYLR
                     HVNPKVIAVTGSNGKTTTKDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEI
                     SILEMGMSGFHEIEFLSNLAQPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDN
                     GTFIYDGDEPLLKPHVKEVENAKCISIGVATDNALVCSVDDRDTTGISFTINNKEHYD
                     LPILGKHNMKNATIAIAVGHELGLTYNTIYQNLKNVSLTGMRMEQHTLENDITVINDA
                     YNASPTSMRAAIDTLGTLTGRRILILGDVLELGENSKEMHIGVGNYLEEKHIDVLYTF
                     GNEAKYIYDSGQQHVEKAQHFNSKDDMIEVLTSDLKAHDRVLVKGSRGMKLEEVVNAL
                     IS"
     gene            16888..18408
                     /locus_tag="PPY92_000721"
     CDS             16888..18408
                     /locus_tag="PPY92_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDJ2624744.1"
                     /translation="MQNFKELGISDNTVQSLESMGFKEPTPIQKDSIPYALQGIDILG
                     QAQTGTGKTGAFGIPLIEKVVGKQGVQSLILAPTRELAMQVAEQLREFSRGQGVQVVT
                     VFGGMPIERQIKALKKGPQIVVGTPGRVIDHLNRRTLKTDGIHTLILDEADEMMNMGF
                     IDDMRFIMDKIPAVQRQTMLFSATMPKAIQALVQQFMKSPKIIKTMNNEMSDPQIEEF
                     YTIVKELEKFDTFTNFLDVHQPELAIVFGRTKRRVDELTSALISKGYKAEGLHGDITQ
                     AKRLEVLKKFKNDQINILVATDVAARGLDISGVSHVYNFDIPQDTESYTHRIGRTGRA
                     GKEGIAVTFVNPIEMDYIRQIEDANGRKMSALRPPHRKEVLQAREDDIKEKVENWMSK
                     ESESRLKRISTELLNEYNDVDLVAALLQELVEANDEVEVQLTFEKPLSRKGRNGKPSG
                     SRNRNSKRGNPKFDSKSKRSKGYSSKKKSTKKFDRKEKSSGGSRPMKGRTFADHQK"
BASE COUNT         6639 a         2828 c         3359 g         5629 t
ORIGIN      
        1 gaaatgtaaa gtaataggag ttctaagtgg aggatttacg atggataaaa tagtaatcaa
       61 aggtggaaat aaattaacgg gtgaagttaa agtagaaggt gctaaaaatg cagtattacc
      121 aatattgaca gcatctttat tagcttctga taaaccgagt aaattagtta atgttccagc
      181 tttaagtgat gtagaaacaa taaataatgt attaacaact ttaaatgctg acgttacata
      241 caaaaaggac gaaaatgctg ttgtcgttga tgcaacaaag actctaaatg aagaggcacc
      301 atatgaatat gttagtaaaa tgcgtgcaag tattttagtt atgggacctc ttttagcaag
      361 actaggacat gctattgttg cattgcctgg tggttgtgca attggaagta gaccgattga
      421 gcaacacatt aaaggttttg aagctttagg cgcagaaatt catcttgaaa atggtaatat
      481 ttatgctaat gctaaagatg gattaaaagg tacatcaatt catttagatt ttccaagtgt
      541 aggagcaaca caaaatatta ttatggcagc atcattagct aagggtaaga ctttaattga
      601 aaatgcagct aaagaacctg aaattgtcga tttagcaaac tacattaatg aaatgggtgg
      661 tagaattact ggtgctggta cagacacaat tacaatcaat ggtgtagaat cattacatgg
      721 tgtagaacat gctatcattc cagatagaat tgaagcaggc acattactaa tcgctggtgc
      781 tataacgcgt ggtgatattt ttgtacgtgg tgcaatcaaa gaacatatgg cgagtttagt
      841 ctataaacta gaagaaatgg gcgttgaatt ggactatcaa gaagatggta ttcgtgtacg
      901 tgctgaaggg gaattacaac ctgtagacat caaaacacta ccacatcctg gattcccgac
      961 tgatatgcaa tcacaaatga tggcattgtt attaacggca aatggtcata aagtcgtaac
     1021 cgaaactgtt tttgaaaacc gttttatgca tgttgcagag ttcaaacgta tgaatgctaa
     1081 tatcaatgta gaaggtcgta gtgctaaact tgaaggtaaa agtcaattgc aaggtgcaca
     1141 agttaaagcg actgatttaa gagcagcagc agccttaatt ttagctggat tagttgctga
     1201 tggtaaaaca agcgttactg aattaacgca cctagataga ggctatgttg acttacacgg
     1261 taaattgaag caattaggtg cagacattga acgtattaac gattaattca gtaaattaat
     1321 ataatggagg atttcaacca tggaaacaat ttttgattat aaccaaatta aacaaattat
     1381 acctcacaga cagccatttt tattaattga taaagtagtt gaatatgaag aaggtcaacg
     1441 ttgtgtggct attaaacaag tatcaggaaa cgaaccattc tttcaagggc attttcctga
     1501 gtatgcggta atgccaggcg tattaattac tgaagcgtta gctcaaacag gtgcggtagc
     1561 tattttaaat agtgaagaaa ataaaggtaa aatcgcttta tttgctggta ttgataaatg
     1621 tcgttttaaa cgtcaagtag tacctggtga tactttaacg ttggaagtag aaatcactaa
     1681 aattaaagga ccaatcggta aaggtaatgc taaagctact gtcgatggtc aacttgcttg
     1741 tagttgtgaa cttacatttg caattcaaga tgtaaaataa aacaaaaaaa acattcaaag
     1801 atttaatgtg ttggcataat ctttgaatgt tttttatttt actcttctaa tttttcatcc
     1861 tttaactttg gtttagactg catcattcga ttaaatgatt tttttaattc ttcaccagat
     1921 aatccatcat caataagttg ttctaataaa ctttcagcat actgttgacg taaagtgtaa
     1981 atatgacatt caaatacgat agacattgtt gttttaaaat ttttaatact tttcacagta
     2041 gattcaaata aagctggatc attggctgga cgcgtgatga cgacacgaat atccaataac
     2101 tgttcattgt cttgatattc cttaaatgtt tcataatgct cattagaaat gacaatttca
     2161 agcaaccaac ccgtgccact ttcctcttta ttaattatta cgccatcttc taattcatat
     2221 tctataattc tgccgtgttc attcacaatt tgaaacctta ctgctttaaa agttttcata
     2281 ttcccacatc ccattagttt aatatttatg atttttgaat tatttagaag atttaaggtc
     2341 atttcatgac taatttcttt aaatataaca aataacgaga tgctatagct attaattata
     2401 taaaatttgc cattttgacc atttttaagc tagtaattaa gatataggca taggaggtga
     2461 atcacacact acatgctaaa taaaatcgta attgtcggga gactgacgaa agacgcacaa
     2521 atatttgaaa aggaggatag aaaaattgca acgttttgtg ttgcaacgca ccgaaattat
     2581 aaagatgaaa atggagaaat cgtctgtgat tacttattct gtaaagcatt tggcaagtta
     2641 gcttctaata tagaaaaata tactaatcaa ggtacattgg ttggtataac tggtcaaatg
     2701 agatcaagaa agtatgataa agacggacaa acacactttg tcactgaatt atatgttgaa
     2761 acaataaaat ttatgtcccc taaatcccaa aataatgaaa ttctctcaga tagtatttta
     2821 gatattgact ctcaaaatat agataatcat gacttattag aaatttaaat ccaattatcc
     2881 taaacatcct taatatacat ttaatccatc ctaaattttt tatagatcta attaacttga
     2941 aatatactca attaaaaacg acttacatga ctagtactgt tgtgtaggtc gtctttttgt
     3001 gtagtttttc taatagaaaa tagacataga ttgcaaaacg attacataaa aaattgcaaa
     3061 taaaaaacat ttttactgtc atgacaattc tcgatagaat catatctgaa attatggggg
     3121 atttcgttgt aatcactgtg taaaaaacaa ggctattttt ttattttgtt gtaacctgca
     3181 cggattttat agatgttaca gtagtctctg taaaactttt agagaaacaa attacttgta
     3241 ggaggatttt aagattatga agaaaacatt actcgcatca tcattagcag taggtttagg
     3301 aatcgtagca ggaaatgcag gtcacgaagc ccatgcaagt gaagcggact taaataaagc
     3361 atctttagcg caaatggcgc aatcaaatga tcaaacatta aatcaaaaac caattgaagc
     3421 tggggcttat aattatacat ttgactatga agggtttact tatcactttg aatcagatgg
     3481 tacacacttt gcttggaatt accatgcaac aggtgctaat ggagcaaaca tgagtgcgca
     3541 agcacctgca actaataatg ttgaaccatc agctgttcaa gctaatcaag tacaatcaca
     3601 agaagttgaa gcaccacaaa atgctcaaac tcaacaacca caagcatcaa catcaaacaa
     3661 ttcacaagtt actgcaacac caactgaatc aaaagcatca gaaggttcat cagtaaatgt
     3721 gaatgctcat ctaaaacaaa ttgctcaacg tgaatcaggt ggcaatattc atgctgtaaa
     3781 tccaacatca ggtgcagctg gtaagtatca attcttacaa tcaacttggg attcagtagc
     3841 acctgctaaa tataaaggtg tatcaccagc aaatgctcct gaaagtgttc aagatgccgc
     3901 agcagtaaaa ttatataaca ctggtggcgc tggacattgg gttactgcat aagccattta
     3961 tgcatagcta atcaatagtt atataagtaa cttttagatc ggaatatatc ggggaattct
     4021 cgatatgttc cggtcttttt tatttcgcaa atatacgtca ttatagaatt attatttaaa
     4081 gataataata caactgagca acaataattg aaaatgttga aaccgtttgc taagattaat
     4141 ttgcaattga atactaagga actactaggg gagcctaatg atatggctga gatgaattgt
     4201 tcagaccctt atgacctgat ttcgttagta ccaacgtagg aaagtagtta ttgaagtgat
     4261 ggggctagta tagcaacatt gccatcaaac tactttcatg ttggaagtag tttttttatt
     4321 tagggaggat tttttacaat ggaattttca caaaaattgt accaagctgc aaagccaatc
     4381 attaatgata tttatgagga tgactttatt cagaagatgt tatccggaga tataggagct
     4441 gacgcattac gccattactt aaaagctgat gctgcgtatt taaaagaatt tacaaactta
     4501 tatgctttgt taattccaaa aatgaatagc atgaatgacg taaaattttt agtagagcaa
     4561 atagaattta tggttgaagg tgaagtactt gcgcatgata ttttagcgca aattgttggt
     4621 gaatcatacg aagaaatcat taaaacaaaa gtttggcctc caagtggcga ccattacata
     4681 aaacatatgt atttccaagc gcatagtcgt gaaaatgcta tttatacaat tgctgcaatg
     4741 gcaccttgtc catatattta tgcagaatta gctaagcgct ctcaaagtga tcataagctt
     4801 aatagagaaa aagatacggc aaaatggttt gatttttata gtactgaaat ggatgacata
     4861 attaacgtat ttgaggcatt aatgaataaa ttagccgaat caatgtctga caaggaattg
     4921 gaacaagtaa agcaagtatt tttagaaagt tgcatacatg agcgtcgatt tttcaatatg
     4981 gctatgacac tagaacaatg ggaatttgga ggaaaagtaa atgattaaac ctaaaatagc
     5041 attaaccatt gcaggtactg atccaacagg tggtgccggc gtaatggctg atttaaaatc
     5101 atttcattca tgtggtgtat atggtatggg cgtcgttaca agtattgttg ctcaaaatac
     5161 attgggcgta caacatattc ataatttaaa tcatcaatgg gtagatgaac aacttgatag
     5221 tgtcttcaat gataccttac ctcatgctat taaaacgggg atgattgcta cagcagatac
     5281 tatggaaacg attcgtcatt atttaatgca acatgaatct attccatatg taattgatcc
     5341 tgttatgttg gcgaaaagcg gtgattcatt aatggataat gacacaaagc aaaacttgca
     5401 gcatacgtta ttgccattag ctgacgtagt aacaccgaat ttaccagaag ctgaagaaat
     5461 aacgggacta accattgata gtgaagaaaa aattatgcag gctggccgca tctttattaa
     5521 tgagattggt agtaaaggta tcatcattaa aggcggtcat tcaaatgata ctgatatagc
     5581 aaaagattat ttatttacta acgaaggtgt tcaaacattt gaaaatgaac gatttaaaac
     5641 aaaacatacg catggaacag ggtgtacatt ttcagcagtt ataacggcag aacttgcaaa
     5701 aggtagacca ttatttgagg ctgtacacaa ggctaaaaag tttatttcaa tgagtataca
     5761 atatacacct gaaatcggcc gtggtagagg accagtgaat cattttgcat atttaaagaa
     5821 agagggatta gacgatgaat tatctaaata aaatacgtat tgaaaaccca ttaacaattt
     5881 gttatacaaa cgatgtagtt aaaaatttta cagcgaatgg tttattaagt attggtgcga
     5941 gccctgccat gagtgaagct cccgaagaag ctgaagaatt ttacaaagtt gcacaagcgc
     6001 tattaatcaa tatcggtact ttaacagcac aaaatgaaca agatattatt gcgattgctc
     6061 aaacggcaaa tgaggcaggc ttacctattg tatttgaccc tgtagctgtt ggtgcttcta
     6121 catatcgaaa gcaattttgt aaattattat tgaaatcagc gaaagtatca gtaattaaag
     6181 gcaatgcatc tgaaatatta gcgttgattg atgatacagc aactatgaaa ggtacagata
     6241 gtgatgctaa tcttgatgcg gttgcaatag cgaaaaaggc ttacgcaata tataaaactg
     6301 caatagtaat cacaggtaaa gaggacgtta ttgttcaaga taataaagcc atcgtattag
     6361 ctaatggatc tccattatta gcacgagtaa ctggagctgg ttgtttatta ggaggcgtta
     6421 ttgctggatt tttatttaga gaaacagaac cagacataga agcgttaatt gaagcggtaa
     6481 gcgtatttaa tattgctgct gaggtagctg ctgaaaatga aaattgtggt ggtcctggta
     6541 cgttttcacc attgttgctt gatacgttat atcatttaaa tgaaacaacc tatcaacaac
     6601 ggattcgtat tcaagaggtg gaataatatg tttaaccaat cgtatctaaa tgtgtatttt
     6661 atttgtggga cctccgatgt tccgagtcat cgaactattc atgaagtatt agaagcagca
     6721 ttaaaggcag ggattacact tttccaattt cgagaaaagg gtgaatcagc tttaaaagga
     6781 aacgataaac ttgtattagc taaagaatta cagcatctct gtcatcaata tgatgtgcct
     6841 tttatcgtta atgatgatgt atccttggca aaagaaatta acgcggatgg tatacatgtc
     6901 ggtcaagatg atgcgaaagt caaagagata gcacaatatt tcactgataa aattattgga
     6961 cttagtatta gtgatttaga tgagtatgca aaatctgatt taactcatgt tgattatata
     7021 ggtgttggac caatctatcc tacgccgtcg aagcatgacg cgcatatacc agtaggtcca
     7081 gaaatgattg caacgtttaa agaaatgaat ccgcaactac cgattgtagc tattggcggt
     7141 atcaatacta ataatgtggc gccgattgta gaagcaggtg ccaatggaat ttcagtcatt
     7201 tcagccattt ctaaaagtga aaatattgaa aagactgtta atcgattcaa agattttttt
     7261 aataattagt taaatttttc cacttattgt ttcatacgta ctcattaatg tgatagaata
     7321 aatcctatgt gaatataatg atagaggtgg aaaaatgaag aaaaaagcgt tactaccatt
     7381 atttttaggt attatggtct ttttggctgg ttgtgactat tctaaacctg aaaaacgtag
     7441 tgggtttttc tacaatacat tcgtagatcc aatgaaaaat gtattggatt ggttgggaaa
     7501 taacttatta aacgacaatt atggtttagc tattattatc cttgtattgg taattcgtat
     7561 tattttatta ccattcatgt tgtcaaacta taaaaatagt catatgatgc gtcaaaaaat
     7621 gaaagttgca aagccagaag ttgaaaaaat tcaagaaaaa gtgaaacgtg cgcgtacaca
     7681 agaagaaaaa atggctgcaa accaagaatt aatgcaagta tataaaaagt atgacatgaa
     7741 cccgattaag agtatgttgg gttgtttacc aatgctaatc caattgccaa tcatcatggg
     7801 attatacttt gtacttaaag accaacttgt agatggtttg tttaaatatc cacacttctt
     7861 atggttcgat ttaggacgtc ctgatatttg gattacaatt attgccggtg ttttatactt
     7921 tatccaagca tatgtatcaa gtaaaacgat gccagacgaa caacggcaaa tgggttacat
     7981 gatgatggtc atttcaccaa ttatgattat ctggatttca ttaagctcag catcagcact
     8041 tggtttgtac tggtctgtca gtgcggcgtt ccttgtagtt caaacacact ttgcgaacat
     8101 ttattatgaa aaagtcgcta aaaaagaagt acaacctttc attgaagcgt atgaaagaga
     8161 gcacaacggc ggcagcaata aaaaaggcaa aaacacacaa gtagtgtcta aaaagaaaaa
     8221 gaaataacct tagcaagtcc aaataatgtg caccctaaaa gttgaagata attttaactt
     8281 tatagggcgc acattatttt tcgcttcaat agcgaaactt agtgtcacat tatacaatat
     8341 gtgataaata gaaacagcag taagatgatt tcaaatgtga aagtcatcct actgctgttt
     8401 ttatttgaaa taatattaac gagatttcag tatgtaaaaa gtggttaata acagagttgt
     8461 cattcttcat cgcaatgtca tcttgacaca atgattcatc agtgtagcag tatatgtcgc
     8521 aaatttccac agtaattcac taatacatgt aacagtgtga aatactaaca tcacaacggc
     8581 ataatacaca ttgcaacgtc ccaacaacat atcatagaat agtaacggca tggcatcaac
     8641 atcaaaccgg tctataacac cataacagcg caacgccaca tcatagcact acatcaagtc
     8701 gcatcgtggt accagcacat caaagtaaca tggcatcaac gccaaaccgg tctataacac
     8761 cataacaccg caatgccaca tcatagcact acatcaagac gcatcgtggt accagcacat
     8821 caaagtagca tggcatcgac gccaaaccgg tctataacac cataacaccg caatgccaca
     8881 tcatagcact acatcaagac gcatcgtggt accagcacat caaagtaaca tgacatgaca
     8941 ccaagctccc acaacaacac atcaaagtaa catggcatga caccaagctt ccacaacaac
     9001 acgccattac atcgcatcat catactacaa cttcaatgtc tagtcgtgat aattccattc
     9061 cgtaaaaaac tgtttcaagt acatcatcat aaagtcgtga cgttctttag caatcatctt
     9121 cgccgtatct gtatgcatta aagattctaa cttaagtaat ttttcaaaga aatgtttaat
     9181 tgcagatggt ggcaactgtt caactaaatc atcattaatc ttatctagtg acatatgttc
     9241 tgtccacatt ggttcaccaa agtgtcctgc aaattgaaat gttcgtgcaa cacctatagc
     9301 gcctatagca tcaagacgat ctgcatccct gacaatttga ccttctaaag ataaagtgac
     9361 atgatcgttt ttgccattgc gatagctcat attattaata ataaataaaa tatgctcttg
     9421 atcttcggtt gatagtgata aagaagataa aaatgacttc aattcaacat attgtttgtt
     9481 agcatctaca actttttcgt caacggtatc atgaagcaaa catgcgagtt caatgactaa
     9541 agtatcatta acaccttcat tttcagcgat tgatttagct aaagcagtga cacgatatac
     9601 atgcgcaata tcatggcctg tataatcatt ttgatgaata gattccatat attttttggc
     9661 agcactaatg acgtctgatt gttgcattaa agttacactc ctcatatttc tattttataa
     9721 gataggtgac aataattgtg aaataccttc tttaaattta atccaaagac ttcgcttagc
     9781 ataacgtgct tttgttaatt cagaagatac tgctaaatca tctataaaag cttgttttaa
     9841 ttttttggca atttgttggt cataaataaa agcgttgact tcgaaattca atgtgaaact
     9901 gcgatggtcc atattagctg ttcccacact tgcaatttca tcatctataa caagtgtttt
     9961 tgagtgtaaa aagccattgt cgtagtgaaa tactttaaca ccggcatcta ataaggatgc
    10021 tgcatttttt aaagtagccc aaaaaacaaa cggatggtca ggtttattag gaatcatgat
    10081 attgacatca acaccaccta atgccgcaat tttaatagaa tctaaaaagg cttgatcagg
    10141 tatgaaatag ggagattgaa tataaatcga ttttttagca gatgaaatca ttttcaaata
    10201 gccgtattta atctgttccc attcttcgtc aggaccacta gaagctattt gaacgccaat
    10261 tgttccacca gaatttacat ctgggaaata acgatcatca taggagatgt ggtcacgtgt
    10321 ggcttgtgaa ttccaatcta gaataaatcg taattgcaat gcattcactg catccccgac
    10381 aattcgtaaa tgcgtatctc gccaatagcc gaattttttt gatttaccta agtactcatc
    10441 accaacatta aaaccaccaa catatccaat ttgcccatct attacaacaa tttttcgatg
    10501 gtttcgattg ttcatacgca agttaattaa aggtaatttt gatgggaaaa atgcttcagc
    10561 atgtccacct ttattgcgaa acgggcgtaa gcctttttta cgcagtccac gagaacccat
    10621 gtcatcataa agaattttaa cttctacacc ttgttccgct tttttaccaa gttcatttaa
    10681 aatggtacga cctaattcat cattttgaat aatatagtac tgaaaatgaa tataatcagt
    10741 agcattacgg atgtcttgta ttaggtcatc aaatttttct tggccgtctg tgtatatttt
    10801 taaatcgttg tctgttgtta aaaatgctgc gttattatat aacaacattt gaatcatttc
    10861 tttaaatttt acaatttgat aattggaatt tgaaaagttt tcatttttta aagcagctaa
    10921 ttgctcatca acgattaact ctaatccttt tttatcttcc ttatcaattt tgaaaatttg
    10981 gtcacgttga atttgtcgtc ctaatagtaa gtataaaatg aagccgaata aaggcaagaa
    11041 aactaagact agtaaccaag cccagataga attggcagaa cgtctttcca tgaaaataat
    11101 ggtaaaggcg aataataagt ttaaaataaa tgcaccaata aatattgaat ttaaaataat
    11161 attagaatgc ttgagtgcaa tggataataa ctctatcatt tgaaacctcc catcgaaaat
    11221 cttaaacgag tagtatatct tattaattgt atcatgaagc aatttaaata tcatgacaat
    11281 ttagtttgaa aaacacactt tgtgttgacg ttgtgtttta tgggatatag tatagtaagg
    11341 aatgtaaatg aaggagtgaa tgctatgact gaacaagata atgcacatca ttctgaacaa
    11401 ataaaaacga atcttaaatc acgtttaaat cgaattgaag gacaagtgag agcgattaat
    11461 cgcatgattg aagaagatgt ctattgtgat gatgtcctta cgcaaataag agcgacacgt
    11521 tcggcgttaa acagtgttgc gataaagtta ttagaacaac atatgaaaag ttgtattatg
    11581 aataaagtta atcaaggtgc tcaggaagag gcaatggaag agttattagt gacttttcaa
    11641 aaattgatta aagactaaag gagtttaaga tgatacatca aaatacgatt tacacagcgg
    11701 gaattgaaac agaagaacaa gtaagtcaat tgacagaacg catttcaaat atgataggtg
    11761 ttcatcaagt gaatattaat ataatagatg gtcaagtaac tgtatcgtat gagacaccag
    11821 caaatttgaa tagtattgaa aaagaaatct atgatgaagg atacaaaatt gtattttagg
    11881 gtataatgta gagtgcgcta tagattttaa ttttgaaaat aaattaaaaa ttttgtaaat
    11941 gatgtagtaa aggtatgtcg aatagggtaa gataatattg aaagcgatcc aaacgaggtg
    12001 ataataatga aacgtcctga aaagattcaa aatgtagtca aactattgtc atcattaggt
    12061 gtgaatatta aaaaaactaa atctcgttta gacattatta atactttgcc agcatctaat
    12121 aaagtaagtc acgaattaaa ataattgact gtgattgtga atcacacacc gttaaagcta
    12181 tcgtcggact attcgatgat agcttttttg actggattta ctaaaatgaa agcaagtact
    12241 atataacaaa atccctcttt agagtgtaca ttttacaatg tgtacttcaa agagggattg
    12301 ttattatcga gtaatatcta tatatgacat tttaaatcga cgacgtaaat agactatata
    12361 acgtcatctt gaaaagttat gatatttgaa actcaaaata gtttaaatta attacttttt
    12421 ggatggtata aatcgacata tcgttttggt tcatgataat aaattgataa gactatacct
    12481 attccagtca tcatactcca tagcgcacta ccaccataac taataaatgg taatggaata
    12541 ccagtgattg gtaacaactg aattgtcata ccaatatttt gtaaaatatg gaacacaagt
    12601 aaagtgacga aaccaacgat aaagattttg ttaaattgat cttcaatttt cgcagctaat
    12661 cttattagat ggaagattaa aaataaaaat attaagatca atatgacaga accgataaag
    12721 ccaagttcct ctccaatcac tgaaaagata aagtcagtat gattttcagg tatataaact
    12781 tcaccgtgat tgtatccttt acctagtaac tgtccagaac cgatagcttt aagtgattca
    12841 gttaaatgat agccatcacc actactatat gtataggggt caagccatga attgattcgt
    12901 cccatttgat acagttggac acctaataaa ttttcaatta atgcgggtgc atatagaata
    12961 cctaaaatga ctgtcattgc accaacaata cctgtaataa agataggtgc taagatacgc
    13021 catgttatac cacttactaa catcacacct gcaataatag cagctaatac taatgtagtt
    13081 cctaggtcat tttgcagtaa tattaaaata cttggtacta acgagacacc aataattttg
    13141 aaaaataata acaaatcact ttggaatgat ttattgaatg tgaattgatt atgtctagaa
    13201 acgacacgcg ctaatgctaa aattaaaata attttcatga attcagatgg ctgaatactg
    13261 atagggccaa acgtgtacca acttttggca ccattgataa taggtgtaat aggtgactca
    13321 ggaataacga gcaagcctat taataataga cagattaaga aatacaataa atatgtataa
    13381 tgtttaatct ttttaggtga aataaacatg atgatacctg caaaaattgc acctaaaatg
    13441 taataaaaaa tttgtctgat accgaaatta gcactgtatt gaccaccgcc cattgccgag
    13501 ttaataagca gaacactgaa aattgctaaa acagctatag tggctactaa tacccagtct
    13561 actttgcgaa gccaatgctt atccggctgt tgacgagatg aataattcat tgcaaactcc
    13621 ttttatactc actaatgttt atatcaattt tacatgactt tttaaaaatt agctagaata
    13681 tcacagtgat atcagctata gatttcaatt tgaattagga ataaaataga agggaatatt
    13741 gttctgatta taaatgaatc aacatagata cagacacata agtcctcgtt tttaaaatgc
    13801 aaaatagcat taaaatgtga tactattaag attcaaagat gcgaataaat caattaacaa
    13861 taggactaaa tcaatattaa tttatattaa ggtagcaaac cctgatatat cattggagga
    13921 aaacgaaatg acaaaagaaa atatttgtat cgtttttgga gggaaaagtg cagaacacga
    13981 agtatcgatt ctgacagcac aaaatgtatt aaatgcaata gataaagaca aatatcatgt
    14041 tgatatcatt tatattacca atgatggtga ttggagaaag caaaataata ttacagctga
    14101 aattaaatct actgatgagc ttcatttaga aaatggagag gcgcttgaga tttcacagct
    14161 attgaaagaa agtagttcag gacaaccata cgatgcagta ttcccattat tacatggtcc
    14221 taatggtgaa gatggcacga ttcaagggct ttttgaagtt ttggatgtac catatgtagg
    14281 aaatggtgta ttgtcagctg caagttctat ggacaaactt gtaatgaaac aattatttga
    14341 acatcgaggg ttaccacagt taccttatat tagtttctta cgttctgaat atgaaaaata
    14401 tgaacataac attttaaaat tagtaaatga taaattaaat tacccagtct ttgttaaacc
    14461 tgctaactta gggtcaagtg taggtatcag taaatgtaat aatgaagcgg aacttaaaga
    14521 aggtattaaa gaagcattcc aatttgaccg taagcttgtt atagaacaag gcgttaacgc
    14581 acgtgaaatt gaagtagcag ttttaggaaa tgactatcct gaagcgacat ggccaggtga
    14641 agtcgtaaaa gatgtcgcgt tttacgatta caaatcaaaa tataaagatg gtaaggttca
    14701 attacaaatt ccagctgact tagacgaaga tgttcaatta acgcttagaa atatggcatt
    14761 agaggcattc aaagcgacag attgttctgg tttagtccgt gctgatttct ttgtaacaga
    14821 agacaaccaa atatatatta atgaaacaaa tgcaatgcct ggatttacgg ctttcagtat
    14881 gtatccaaag ttatgggaaa atatgggctt atcttatcca gaattgatta caaaacttat
    14941 cgagcttgct aaagaacgtc accaggataa acagaaaaat aaatacaaaa ttgactaact
    15001 gaggttgtta ttatgattaa tgttacatta aagcaaattc aatcatggat tccttgtgaa
    15061 attgaagatc aatttttaaa tcaagagata aatggagtca caattgattc acgagcaatt
    15121 tctaaaaata tgttatttat accatttaaa ggtgaaaatg ttgacggtca tcgctttgtc
    15181 tctaaagcat tacaagatgg tgctggggct gctttttatc aaagagggac acctatagat
    15241 gaaaatgtaa gcgggcctat tatatgggtt gaagacacat taacggcatt acaacaattg
    15301 gcacaagctt acttgagaca tgtaaaccct aaagtaattg ccgtcacagg gtctaatggt
    15361 aaaacaacga ctaaagatat gattgaaagt gtattgcata ccgaatttaa agttaagaaa
    15421 acgcaaggta attacaataa tgaaattggt ttacctttaa ctattttgga attagataat
    15481 gatactgaaa tatcaatatt ggagatgggg atgtcaggtt tccatgaaat tgaatttctg
    15541 tcaaacctcg ctcaaccaga tattgcagtt ataactaata ttggtgagtc acatatgcaa
    15601 gatttaggtt cgcgcgaggg gattgctaaa gctaaatctg aaattacaat aggtctaaaa
    15661 gataatggta cgtttatata tgatggcgat gaaccattat tgaaaccaca tgttaaagaa
    15721 gttgaaaatg caaaatgtat tagtattggt gttgctactg ataatgcatt agtttgttct
    15781 gttgatgata gagatactac aggtatttca tttacgatta ataataaaga acattacgat
    15841 ctgccaatat taggaaagca taatatgaaa aatgcgacga ttgccattgc ggttggtcat
    15901 gaattaggtt tgacatataa cacaatctat caaaatttaa aaaatgtcag cttaactggt
    15961 atgcgtatgg agcaacatac attagaaaat gatattactg tgataaatga tgcctataat
    16021 gcaagtccta caagtatgag agcagctatt gatacactgg gtactttgac agggcgtcgc
    16081 attctaattt taggagatgt tttagaatta ggtgaaaata gcaaagaaat gcatatcggt
    16141 gtaggtaatt atttagaaga aaagcatata gatgtgttgt atacgtttgg taatgaagcg
    16201 aagtatattt atgattcggg ccagcaacat gtcgaaaaag cacaacactt caattctaaa
    16261 gacgatatga tagaagtttt aacaagcgat ttaaaagcgc atgaccgtgt attagttaaa
    16321 ggatcacgtg gtatgaagtt agaagaagtt gtaaatgctt taatttcata gagattagtc
    16381 gagcgacctt ttacttataa aaatgatttg aattaatact aaaagattac aaagaagagg
    16441 tggttttgtg tgtaaataca aaattgcctt tttcttttta tgttaaatct ataaatttga
    16501 aactaaatca aggttaattc tatgtacaca ctttatatag gaagtagttt gaatgtttat
    16561 ataatgtttt acaaaaagat gtagtattat aatgtctaat ttcacatgtg tttcagtaaa
    16621 atttgttgtg gaatgttaac gatatacgta ttttataaaa aattttttat aatgattatt
    16681 cgaatgatgc gtaacgctta catcttatct aatgctagct ttttgacaaa aatatgacaa
    16741 tcaattaatg tgattctaat aaatattcgc aaattgcttt attgcgatta aatttttttg
    16801 gtggtactat atagaagttg atgaaatatt aatgaactta tatgcaaaag tatattgaga
    16861 aataaacagg taaaaaggag aattattttg caaaatttta aagaactagg gatttcggat
    16921 aatacggttc agtcacttga atcaatggga tttaaagagc cgacacctat ccaaaaagac
    16981 agtatccctt atgcgttaca aggaattgat atccttgggc aagctcaaac cggtacaggt
    17041 aaaacaggag cattcggtat tcctttaatt gagaaagtag tagggaaaca aggggttcaa
    17101 tcgttgattt tagcacctac aagagaattg gcaatgcagg tagctgaaca attaagagaa
    17161 tttagccgtg gacaaggtgt ccaagttgtt actgtattcg gtggtatgcc tatcgaacgc
    17221 caaattaaag ccttgaaaaa aggcccacaa atcgtagtcg gaacacctgg gcgtgttatc
    17281 gaccatttaa atcgtcgcac attaaaaacg gacggaattc atactttgat tttagatgaa
    17341 gctgatgaaa tgatgaatat gggattcatc gatgatatga gatttattat ggataaaatt
    17401 ccagcagtac aacgtcaaac aatgttgttc tcagctacaa tgcctaaagc aatccaagct
    17461 ttagtacaac aatttatgaa atcaccaaaa atcattaaga caatgaataa tgaaatgtct
    17521 gatccacaaa tcgaagaatt ctatacaatt gttaaagaat tagagaaatt tgatacattt
    17581 acaaatttcc tagatgttca tcaacctgaa ttagcaatcg tattcggacg tacaaaacgt
    17641 cgtgttgatg aattaacaag tgctttgatt tctaaaggat ataaagctga aggcttacat
    17701 ggtgatatta cacaagcgaa acgtttagaa gtattaaaga aatttaaaaa tgaccaaatt
    17761 aatattttag tcgctactga tgtagcagca agaggactag atatttctgg tgtgagtcat
    17821 gtttataact ttgatatacc tcaagatact gaaagctata cacaccgtat tggtcgtacg
    17881 ggtcgtgctg gtaaagaagg tatcgctgta acgtttgtta atccaatcga aatggattat
    17941 atcagacaaa ttgaagatgc aaacggtaga aaaatgagtg cacttcgtcc acctcatcgt
    18001 aaagaagtac ttcaagcacg tgaagatgac atcaaagaaa aagttgaaaa ctggatgtct
    18061 aaagagtcag aatcacgctt gaaacgcatt tctacagagt tgttaaatga atataacgat
    18121 gttgatttag ttgctgcact tttacaagag ttagtagaag cgaacgatga agttgaagtt
    18181 caattaactt ttgaaaaacc attatctcgc aaaggccgta atggtaaacc aagcggttct
    18241 cgtaacagaa atagtaagcg tggtaatcct aaatttgaca gtaagagtaa acgttcaaaa
    18301 ggatactcaa gtaagaagaa aagtacaaaa aaattcgacc gtaaagagaa gagcagcggt
    18361 ggaagcagac ctatgaaagg tcgcacattt gctgaccatc aaaaataatt tatagattaa
    18421 gagcttaaag atgtaatgtc ttgagctctt ttttt
//
LOCUS       DANBWH010000030        18445 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.030, whole genome shotgun sequence.
ACCESSION   DANBWH010000030 DANBWH010000000
VERSION     DANBWH010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18445
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.030"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(4..1173)
                     /locus_tag="PPY92_000722"
     CDS             complement(4..1173)
                     /locus_tag="PPY92_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDJ2624745.1"
                     /translation="MIKNTIKKLIEHSIYTTFKLLSKLPNKNLIYFESFHGKQYSDNP
                     KALYEYLTEHSDAQLIWGVKKGYEHIFQQYNVPYVTKFSMKWFLAMPRAKAWMINTRT
                     PDWLYKSPRTTYLQTWHGTPLKKIGLDISNVKMLGTNTQNYQDGFKKESQRWDYLVSP
                     NPYSTSIFQHAFHVSRDKILETGYPRNDKLSHKRNDTEYINGIKTRLNIPLDKKVIMY
                     APTWRDDEAIREGSYQFNVNFDIEALRQALDDDYVILLRMHYLVVTRIDEHDDFVKDV
                     SDYEDISDLYLISDALVTDYSSVMFDFGVLKRPQIFYAYDLDKYGDELRGFYMDYKKE
                     LPGPIVENQTALIDALKQIDETANEYIEARTVFYQKFCSLEDGQASQRICQTIFK"
     gene            complement(1601..3295)
                     /locus_tag="PPY92_000723"
     CDS             complement(1601..3295)
                     /locus_tag="PPY92_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDJ2624746.1"
                     /translation="MTKTKQAIHIDNIYWERVQLYIEGHSEGVDLTSGQFVLRNLTET
                     KTLEANEMKIDGNTFICRFNVAILDDGYYLPMDKYLFVYHDQLEYIGQLNPNIIDQAY
                     AALNEEQIEEYNELTTQNGKVNYLLAYDAKVFRKGGVSQHTVYTITPEIASDVNEFVF
                     DIEITLPQEKSGVIATSAHWLHKQGHKASFESRSFLFKAIFNITKLLHIKRSKTILFT
                     SDSRPNLSGNFKYVYDELLRQKVDFDYDIKTVFKANITDRRKWRDKFRLPYLLGKADY
                     IFVDDFHPLIYTVRFRPSQEIIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKA
                     YVSSETDIPFYAEAFGIREENVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKV
                     ILFAPTFRGNGHGTAHYPFFKIDFERLARYCEKHNAVVLFKMHPFVKNRLNISREHRQ
                     YFIDVSDHREVNDILFVTDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEP
                     YESFVPGKIVQSFDALMDALDTEDYEVEKVVPFLDKHFKYQDGRSSERLVKDLFRR"
     gene            complement(3317..4342)
                     /locus_tag="PPY92_000724"
     CDS             complement(3317..4342)
                     /locus_tag="PPY92_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDJ2624747.1"
                     /translation="MINQVYQLVAPRQFDVTYNNVDIYGNHVIVRPLYLSICAADQRY
                     YTGRRDENVLRKKLPMSLVHEAVGEVVFDSKGVFEKGTKVVMVPNTPTEQHHIIAENY
                     LASSYFRSSGYDGFMQDYVVMAHDRIVPLPNDIDLSTISYTELVSVSYHAIQRFERKS
                     IPLKNSFGIWADGNLGYITAILLRKLYPEAKIYVFGKTDYKLSHFSFVDDIFTVNQIP
                     DDLKIDHAFECVGGKGSQVALQQIVEHISPEGSIALLGVSELPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFEQVVDLYRKYPDIVEKLALLKGHEINVCTMQDIVQAFEMDLSTSWGK
                     TVLKWTI"
     gene            complement(4335..5051)
                     /locus_tag="PPY92_000725"
     CDS             complement(4335..5051)
                     /locus_tag="PPY92_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDJ2624748.1"
                     /translation="MIYAGILAGGIGSRMGNVPLPKQFLDIDNKPILIHTIEKFILVS
                     EFNEIIIATPAQWISHTQDILKKYNITDQRVKVVAGGTDRNETIMNIIDHIRNVNGIN
                     NDDVIVTHDAVRPFLTKRIIKENIEVAAKYGAVDTVIEAIDTIVMSKDKQNIHSIPVR
                     NEMYQGQTPQSFNIKLLQDSYRALSSEQKEILSDACKIIVESGHAVKLVRGELYNIKV
                     TTPYDLKVANAIIQGDIADD"
     gene            complement(5579..6622)
                     /locus_tag="PPY92_000726"
     CDS             complement(5579..6622)
                     /locus_tag="PPY92_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDJ2624749.1"
                     /translation="MKALKLYGVEDLRYEDNEKPVIESANDVIVKVRATGICGSDTSR
                     YKKMGPYIKGMPFGHEFSGVVDAIGSDVTHVNVGDKVTGCPAIPCYQCEYCLKGEYAR
                     CEKLFVIGSYEPGSFAEYVKLPAQNVLKVPDNVDYIEAAMVEPSAVVAHGFYKSNIQP
                     GMTVAVMGCGSIGLLAIQWARIFGAAHIIAIDIDAHKLDIATSLGAHQTINSKEENLE
                     KFIENHYANQIDLAIESSGAKVTIGQILTLPKKGGEVVLLGIPYDDIEIDRVHFEKIL
                     RNELTVCGSWNCLSSNFPGKEWTATLHYMKTKDINVKPIISHFLPLEKGPETFDKLVN
                     KKERFDKVMFTIY"
     gene            complement(6646..6792)
                     /locus_tag="PPY92_000727"
     CDS             complement(6646..6792)
                     /locus_tag="PPY92_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624750.1"
                     /translation="MVESMLTFMLGPLRQITDFYMEHLLVSNSIVIAGYFATGIFKKK
                     KVVN"
     gene            complement(6794..7849)
                     /locus_tag="PPY92_000728"
     CDS             complement(6794..7849)
                     /locus_tag="PPY92_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDJ2624751.1"
                     /translation="MKALVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGICGTDIH
                     TYEGHYKVNFPVTLGHEFSGEIVEVGADVKDFKVGDRVTSETTFYVCNECEYCKSKDY
                     NLCNHRKGIGTQVDGVFTNYVIAREESLHHIPDEVSYQSAAMTEPLACAHHGVSKIQV
                     NSGDVAVVMGPGPIGLLVAQVLKSKGATVVVTGLDNDKVRLDKAEALHMDYVVNLQQT
                     DLKTYINGITDGYGADVVVECSGAVPAARQGLDIIRKKGFYSQIGIFKDAEIPFDMEK
                     VIQKEITVVGSRSQKPADWEPSLQLMADGLVNAEALVTKIYDISKWDEAYQHLKSGEG
                     IKALLKPLDLDENEGEN"
     gene            complement(7867..9126)
                     /locus_tag="PPY92_000729"
     CDS             complement(7867..9126)
                     /locus_tag="PPY92_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDJ2624752.1"
                     /translation="MSYFTDFVRGFLDLGATVILPVVIFLLGLFFRQKIGAAFRSGLT
                     IGVAFVGIFLVIDLLVKNLGPAAQAMVKNLGVSLNVIDVGWPATSSIAWASSVAAFII
                     PLGIIVNVVLLVTKVTKTMNVDIWNFWHYTFTAAMVYAVSGSIWQALLAAVIFQVICL
                     KVADWTAPMMSEFFDLPGVSIATGSTISYAPGIYLVKLLQKVPGLNKLDADPETIQKR
                     FGAFGESIFVGLILGLGIGVLAGYKPGDIINLGMSMAAVMVLMPRMVKILMEGLMPVS
                     ESARTWLNKRFGEREIYIGLDAAVALGHPAVISTALILVPITVLLAVILPGNQVLPFG
                     DLATIPFVVAFIVGAARGNIIHSVIVGTIMIAISLYIATDVAPIFTDMAKGTNVQMPK
                     GSSEISSIDQGGNIVNYLIFKLFSLFN"
     gene            complement(9353..9631)
                     /locus_tag="PPY92_000730"
     CDS             complement(9353..9631)
                     /locus_tag="PPY92_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDJ2624753.1"
                     /translation="MKQVLVACGAGIATSTVVNNAIEEMAKEHNIKVDIKQIKITEVG
                     PYEDTADLLVTTAMTKKEYKFPVINARNFLTGIGIEETKQQILTELQK"
     gene            complement(9654..10121)
                     /locus_tag="PPY92_000731"
     CDS             complement(9654..10121)
                     /locus_tag="PPY92_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDJ2624754.1"
                     /translation="MGQQLVHKENIMLNLSATDKESVLSQMSDVLFQNGFVKSTFKDA
                     VIDREKEFATGLPTHLCSVAIPHTDVEHINHRTIGVAVLEKEVPFIEMGTLDQQTEVK
                     IVFMLAMDKVDDQLKLLQQLMQIFQSEEKLEQILRTKDETILATLINDYLEYN"
     gene            complement(10106..12202)
                     /locus_tag="PPY92_000732"
     CDS             complement(10106..12202)
                     /locus_tag="PPY92_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDJ2624755.1"
                     /translation="MNGDNQQILREIVLNPTIHGKELESIFGLSRRQLGYRIQKINLW
                     LEQEGYPKLERTSQGNFIVSSEIMTLFKRDVSEQQMLNGNNVIFSIETRRYYLMLMLF
                     SKENAMSLNHFSIDLQVSKNTVIHDINHVKEQLEDHGLSLKYSRKHGYEIVGDEFEVR
                     RFFIKLIDQRLNHDITKSEVLKALNLTFEDIAYQKDKIKQVEQFLKSRFIDKSLSSLP
                     YVLCVIRRRIQSGHVMNPLNINYQYLRDTKEYQATEIMTQHEPDLPEAEKLYLTLHLL
                     STSVQWTDLQESDNISNLTMAIAQMIHHFEQITFINIEDKEKLSQQLLLHLTPAFYRI
                     KYNLTDRDELINPLQGNYQSLFHMVKQSCQSLTEYFGKSLPDNEIAYLTMLFGGSLRR
                     QDENFDGKIKAIIVCTQGTSVSQMMLYELRNLFPEIIFLDAISLRTFENYTLDYDIVF
                     SPMFVLTHKKLFITKVALSENEQRKLRKEVMKYINKESADIDKEINKLMALIERTTTV
                     NDITELRDGLEDFIANYNSISTINGSIVTQNKTLDLADLIPARHVKRMHHVENIDEAI
                     AKASDVLVANHFIDIKYIHEMQQVFDDSYMVIMQNIAIPHAYSEKHVHKTAMSMLILQ
                     EPIYMSDGTAIHIIVPIAAVDKVTHLRALLQLRDVAQDNDAIKRIIQSRKNSDVNEIL
                     KNYSNKEARENGWDSN"
     gene            complement(12333..12430)
                     /locus_tag="PPY92_000733"
                     /pseudo
     CDS             complement(12333..12430)
                     /locus_tag="PPY92_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498807.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12540..13475)
                     /locus_tag="PPY92_000734"
     CDS             complement(12540..13475)
                     /locus_tag="PPY92_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDJ2624756.1"
                     /translation="MKRKIIMDCDPGHDDAIALILAGAIDSPLEILAVTTVAGNQSVD
                     KNTTNALNVLDIMGRQDIAVAKGADRPLIKPAAFASEIHGESGLDGPKLPSTPSRQAV
                     AMPASDVIINKVMTSDTPVTIVATGPLTNVATALIREPRIAEHIESITLMGGGTFGNW
                     TPTAEFNIWVDAEAAKRVFESGITINVFGLDVTHQVLADNHVIERFESINNPVAQFVV
                     ELLQFFKKTYKTHFNMDGGPIHDACTILYLLQPELFTMVPVNIDIEHQSPLTYGTMAV
                     DLNHVTGKPANAYFATAVDVEEVWNLIDHKLRTYE"
     gene            13837..15366
                     /locus_tag="PPY92_000735"
     CDS             13837..15366
                     /locus_tag="PPY92_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDJ2624757.1"
                     /translation="MKSLFEKAQQFGKSFMLPIAILPAAGLLLGIGGALSNPNTVKAY
                     PILDITLLQNIFTLMSAAGSIVFQNLPVIFAIGVAIGLSRSDKGTAGLAALLGFLIMN
                     ATMNGLLTITGTLAKDQLAQNGQGMVLGIQTVETGVFGGIITGIMTAILHNKYHKVVL
                     PPYLGFFGGSRFVPIVTAFAAIFLGVLMFFIWPSIQAGIYHVGGFVTKTGAIGTFVYG
                     FILRLLGPLGLHHIFYLPFWQTALGGTLEVKGHLVQGTQNIFFAQLGDPDVTKYYSGV
                     SRFMSGRFITMMFGLCGAALAIYHTAKPEHKKVVGGLMLSAALTSFLTGITEPLEFSF
                     LFVAPILYVIHAFFDGLAFMMADIFNITIGQTFSGGFIDFLLFGVLQGNSKTNYLYVI
                     PIGIVWFCLYYIVFRFLITKFNFKTPGREDKAAAQQVEATERAQTIVAGLGGKDNIEI
                     VDCCATRLRVTLNQNDKVDKVLLESTGAKGVIQQGTGVQVIYGPHVTVIKNEIEELLG
                     D"
     gene            complement(15686..16639)
                     /locus_tag="PPY92_000736"
     CDS             complement(15686..16639)
                     /locus_tag="PPY92_000736"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDJ2624758.1"
                     /translation="MNKFKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDTEKV
                     RGDVMDLKHATPYSPTTVRVKAGEYSDCHDADLVVICAGAAQKPGETRLDLVSKNLKI
                     FKSIVGEVMASKFDGIFLVATNPVDILAYATWKFSGLPKERVIGSGTILDSARFRLLL
                     SEAFDVAPRSVDAQIIGEHGDTELPVWSHANIAGQPLKTLLEQRPEGKAQIEQIFVQT
                     RDAAYDIIQAKGATYYGVAMGLARITEAIFRNEDAVLTVSALLEGEYDEEDVYIGVPA
                     VINRNGIRNVVEIPLNDEEQSKFAHSAKTLKDIMAEAEELK"
     gene            complement(16950..17057)
                     /locus_tag="PPY92_000737"
     CDS             complement(16950..17057)
                     /locus_tag="PPY92_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624759.1"
                     /translation="MSVCTPTIARIYLTNSKNIVVFLEKSKHDNKNVKV"
     gene            17212..18357
                     /locus_tag="PPY92_000738"
     CDS             17212..18357
                     /locus_tag="PPY92_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide dioxygenase"
                     /protein_id="HDJ2624760.1"
                     /translation="MLTEQEKDIIKQTVPLLKEKGTEITSIFYPKMFKAHPELLNMFN
                     QTNQKRGMQSSALAQAVMAAAVNIDNLSVIKPVIMPVAYKHCALQVYAEHYPIVGENL
                     LKAIQDVTGLEEHDPVIQAWAKAYGVIADVFIQIEKEIYDQMMWIGFKPFKITNIKQE
                     SEDIKSFTVETEEYDFSEFTPGQYITVDVSSDKLPYRAKRHYSIVSGEKNHLTFGVKR
                     DVTTEHEGEVSTILHDEIKEGDMINLAAPVGGFVLENTTEPQLFLGSGIGVTPLVAMY
                     EAASAKGLDTQMVQVAENEQHLPFKDNFNSIASHYDNAKLYTHLKDKQGYIGTEELQA
                     FLANKPEIYICGGTKFLQSMIEALKSLNYDMDRVHYETFIPRLSVAV"
BASE COUNT         5699 a         3575 c         2804 g         6367 t
ORIGIN      
        1 ttatcactta aaaatcgttt ggcaaattcg ttgtgacgct tgtccatctt ctaatgaaca
       61 gaatttttga taaaataccg ttcgtgcttc aatatactca tttgcagtct catcgatttg
      121 ttttaatgca tcaatgagtg ctgtttgatt ttcaacaatt ggacctggca actctttttt
      181 ataatccatg taaaaacctc taagctcatc gccatattta tctaagtcat atgcatagaa
      241 aatttgcgga cgctttaata caccgaagtc gaacatgaca gatgagtagt cggtaactaa
      301 cgcatcgctg attaagtata aatccgaaat gtcttcataa tctgaaacgt ctttcacaaa
      361 atcatcatgt tcatcaatac gtgtcacaac taaataatgc atgcgtaata aaataacata
      421 atcatcatcc agcgcttgac gcaaagcttc tatatcaaag ttaacattaa attgatatga
      481 accttctcga atcgcttcat cgtcacgcca agttggcgcg tacataatca cttttttatc
      541 taatggaata tttaatcttg tcttaatacc attaatatat tcagtatcat tgcgtttatg
      601 tgataattta tcatttcttg gataacctgt ttccaaaatc ttatctcgac taacatgaaa
      661 tgcatgttga aatatcgatg tcgaatatgg attaggtgac actagataat cccaccgttg
      721 gctttctttt ttaaagccat cttggtaatt ttgagtattt gttcctagca ttttaacgtt
      781 actaatatcc aaaccaatct tttttaatgg cgtgccatgc catgtttgta agtacgtcgt
      841 tcgcggtgat ttatataacc aatctggtgt acgtgtgtta atcatccacg ctttcgctct
      901 tggcatcgct aaaaaccatt tcattgaaaa ctttgtaaca tatggtacat tgtactgttg
      961 gaatatgtgt tcatatcctt ttttcacacc ccatattaat tgggcatcgc tatgttcagt
     1021 taagtattca tataatgctt tggggttgtc gctgtattgt ttaccatgaa agctttcaaa
     1081 ataaattaga ttcttgtttg gcaattttga tagtaattta aaagtcgtat atatactatg
     1141 ttctatcaat tttttaattg tatttttaat catgtcgtac ctccgacgtg tttttgtaat
     1201 tatattaata tgtatgagca agctcattgt aaccatgcct attatagcat ttcatcataa
     1261 aatacattta accattacac ttgtcgttaa ttatcatacg aaatacatga ttaatgtacc
     1321 actttaacat aacaaaaaat cgttatccat tcataacgta tgtgtttaca catttatgaa
     1381 ttagataacg attggatcga ttattttatt ttacaaaatg acaattcagt tggaaggtga
     1441 ttgcttttga ttgaatcgcc ttatgcatga aaaatcaaaa ggttattctc attgtatagt
     1501 cctgcttctc atcatgacat gttgctcact tcattgtcag aacccttctt gaaaactatg
     1561 ccttatgact catttgcatg gcaagtaata tatgccaaca ttagcgtcta aacaaatctt
     1621 tgactaaacg ttcacttgag cgaccatctt gatatttaaa atgtttatct aagaatggta
     1681 caactttttc aacctcataa tcttcagtgt ccaaagcatc cattaatgca tcaaaggatt
     1741 gtacaatttt acctggaaca aatgattcat atggttcata gaaatcacgc gtcgtaatgt
     1801 aatcttctaa gtcaaatgca tagaaaatca tcggcttttt aaatactgca tattcatata
     1861 ttaaagatga ataatcacta atcaacaagt ctgtaacaaa gagaatatcg ttaacttcac
     1921 gatgatctga cacatcgata aagtattgtc tatgttcacg tgaaatatta agtctatttt
     1981 ttacgaacgg atgcattttg aataacacaa ctgcattatg cttctcgcag tatcttgcta
     2041 aacgttcaaa atcaatttta aaaaatggat aatgtgccgt accgtgacca ttacctctaa
     2101 atgtcggtgc gaatagaata actttcttac cttttataat tggcaattca tcttccatct
     2161 cttgtttaat ttgtgttgca taagcttcat caaatagtac atcagtacgt ggtacacctg
     2221 ttggtacaac attttcttct ctaattccaa atgcttcagc ataaaatgga atatcggttt
     2281 ctgatgaaac atatgctttc gtgtaactac gatggtttaa tgaatcgata aacggaccac
     2341 cttttttacc tgtacgacta aagccaactg ttttaaaggc accaacagca tgccacactt
     2401 gaataatttc ttgtgatggt ctaaagcgaa ccgtataaat taatggatgg aaatcatcaa
     2461 caaaaatata atcggcctta ccaagtaaat atggcaatct aaacttgtct ctccatttgc
     2521 gtctatccgt aatattcgcc ttaaataccg ttttaatatc ataatcaaaa tctacttttt
     2581 ggcgtagtaa ctcatcatat acatacttga aattccctga taaattcgga cgcgaatctg
     2641 atgtgaataa tattgttttg cttcttttaa tatgtagtaa ctttgtaata ttaaaaatag
     2701 ctttaaataa gaaacttcta ctttcaaatg aagctttatg accttgttta tgaagccagt
     2761 gtgcacttgt cgcaatgacc cctgatttct cttgaggtaa ggtgatttca atatcaaata
     2821 caaattcgtt aacgtcactt gctatttccg gagtaatggt atagaccgta tgttgtgata
     2881 cgccaccttt acggaaaact ttagcatcat acgctaataa atagttcact tttccatttt
     2941 gtgtagtcag ctcattgtat tcttcaattt gctcttcatt taatgccgca taagcttgat
     3001 caataatatt tggattaagt tgtccaatat actctaactg gtcatgataa acaaataaat
     3061 atttatccat tggtaaataa tacccatcgt ctaatattgc gacgttgaat ctacatataa
     3121 atgtattacc gtctattttc atttcatttg cttctaatgt ttttgtttcg gttaaattcc
     3181 tcagaacaaa ttgtcctgat gttaaatcga caccttcact atgtccttca atatataact
     3241 gaacacgttc ccagtatatg ttatcaatat gtattgcttg tttcgttttt gtcattgtta
     3301 ttcccccatc ggtttattaa atcgtccatt tcaatactgt ttttccccaa gatgtcgata
     3361 aatccatttc aaacgcttgg acgatatctt gcatcgtaca tacattaatt tcatgtcctt
     3421 ttaataatgc taacttttca actatgtctg ggtacttacg atataaatca acaacttgct
     3481 caaaatcttt agagccgctt cgactactac caatcaaggt taatcctttt tcaagtacta
     3541 atcgtgtatt cacttccacg ggtaattcac ttacgcctaa caaagcaata ctgccttctg
     3601 gtgaaatatg ttcaactatt tgttgaagtg caacttgact tcctttacct ccaacacatt
     3661 caaatgcatg atcaatttta agatcatctg gtatttgatt tactgtaaag atgtcatcta
     3721 caaatgaaaa atgacttaat ttatagtctg tcttaccaaa tacataaatt ttagcttctg
     3781 ggtacaactt acgtagcaaa atagcagtaa tataacctaa gttaccatca gcccaaatac
     3841 caaagctgtt tttcaaaggt atagatttac gttcaaatcg ttgtatagca tgataactta
     3901 ctgacactaa ctctgtgtat gaaatcgtac tcaaatcaat gtcattaggc agcggaacga
     3961 tacgatcatg tgccatcaca acgtagtctt gcataaaacc atcataacca ctagatctaa
     4021 aataactaga ggctaagtaa ttctccgcga taatatgatg ttgctctgta ggtgtattcg
     4081 gcaccattac tactttcgta cctttttcaa atactccttt actatcgaat acaacttcac
     4141 caacagcttc atgaactaat gacattggca attttttgcg cagtacattt tcatctcttc
     4201 gacctgtgta atacctttga tcagctgcac aaatagacaa gtataaaggt cttacgatga
     4261 catgattacc ataaatatca acattattat atgtgacgtc gaactgtctc ggtgcaacga
     4321 gttgatatac ttgattaatc atcggcaata tcaccttgaa taatggcatt tgctactttt
     4381 aaatcatacg gtgttgtcac tttaatgttg tatagttctc cacgtaccaa tttaactgca
     4441 tgtccagatt cgacaattat tttacatgca tctgataaga tttctttttg ttcactactt
     4501 aaggcgcgat aactatcttg taataattta atattaaatg attgtggtgt ttggccttga
     4561 tacatttcat tccttacagg gatactgtgt atgttctgtt tatctttaga cattacaatc
     4621 gtatcaattg cttcaatgac tgtatctact gcaccatatt ttgctgctac ttcaatgttc
     4681 tctttaataa tacgtttagt taaaaatggt cttacggcat catgagttac aatcacatca
     4741 tcattattaa ttccatttac attgcgaata tggtcgataa tgttcataat tgtttcgttt
     4801 cgatccgtac cacctgcaac tactttgaca cgttgatctg taatgttata tttttttaaa
     4861 atatcctgtg tatgggaaat ccactgtgct ggcgttgcga taataatctc attaaattca
     4921 ctcactaaaa tgaacttctc aattgtatgg attaaaatcg gtttattatc aatatctaaa
     4981 aattgttttg gtaatggcac gttccccatt ctcgaaccaa tacctcctgc taaaatacct
     5041 gcataaatca tgttgtcctc cattctgtca ttacatcatt tccatttata cattactgac
     5101 ctatgcccgc acataagcct aacctattgc tcacttgcct cttttattaa tccaaagata
     5161 gttgtcacaa tagtgtgata attttttata aaaatgtatt tttgtaactg accattctaa
     5221 gttgttttgc catgcagtta atcattaact ctgacgatat taaattgtta aaggtattaa
     5281 tgtttactct ttttcaaatt cattattact gccatcattt taccatatat tataataaat
     5341 ttatcttatt aagtggctgt acttgatttt cactttaaaa attatcaaat attgccatct
     5401 cattttaagt atacaaaatg caaaacaacc gattcacaag catatttcac acaagtaaac
     5461 cggctattta tcaacgtata ttcgaagatg aattatttcg atagtatctg tagaccagac
     5521 ggcattcgca ctttcatagc tataactata ccagcgtttt cgtcctcaaa ggtgcatact
     5581 aataaatcgt aaacatgact ttatcaaatc gttctttctt gttaactaat ttatcaaatg
     5641 tctccgggcc tttttctaac ggtaaaaaat gagaaataat aggctttaca ttaatatctt
     5701 tcgtcttcat ataatgtaag gttgccgtcc actctttgcc cggaaaatta ctggacaaac
     5761 agttccaaga gccacatact gtcaactcgt tacgcagaat tttttcaaaa tgaacgcgat
     5821 caatctcaat atcatcatat ggtattccga gtaataccac ctcgccacct tttttaggta
     5881 gcgtcaatat ttgaccaatc gtaactttag cacctgatga ttctatagct aaatcgattt
     5941 gattggcgta atgattttcg atgaatttct caagattttc ttcttttgaa ttgattgttt
     6001 gatgtgcgcc caatgatgtt gcaatatcta gtttatgcgc atctatatct atagcgatga
     6061 tatgtgcagc accaaatatt cgtgcccatt gaatagctaa caaacctata ctgccacacc
     6121 ccattactgc aacagtcata ccaggttgta tattcgattt ataaaaccca tgcgcaacaa
     6181 cggctgatgg ctcaaccatt gctgcttcaa tgtaatcaac attgtctgga acctttaaaa
     6241 cattttgcgc tggcaatttg acatattccg cgaacgatcc aggttcatat gagccaatga
     6301 cgaataactt ttcacatcgt gcatattcac ctttcaaaca atactcgcat tgataacaag
     6361 gtattgctgg gcaacctgtc actttgtcgc ccacattaac atgcgtaaca tcacttccaa
     6421 tggcatctac tacacctgaa aattcatgac caaatggcat acctttaatg tatggcccca
     6481 tttttttgta tcgtgacgtg tctgaaccac atatgccagt cgctcgtact ttaacaataa
     6541 cgtcattcgc actttcaatg actggctttt cattatcctc ataccgtaaa tcttctacgc
     6601 catataattt caatgctttc acttgtaaat cacctcaaat ttgatttaat tcacaacttt
     6661 tttcttttta aaaatacctg tcgcaaaata acctgcaatg acgatggaat tacttacgag
     6721 taaatgttcc atataaaaat cagtgatttg tcttaatggc ccaagcataa aagttagcat
     6781 tgattctacc atattaattc tctccttcat tttcatctaa atcgagcggc ttaagtaatg
     6841 ctttaatacc ttcgccagat tttaaatgtt gatacgcctc gtcccatttc gaaatatcat
     6901 atatttttgt caccaaagct tcagcattta ctaaaccatc cgccataagt tgcaatgaag
     6961 gttcccaatc tgctggcttt tgacttctac taccaacaac tgttatttct ttttgaatca
     7021 ctttttccat atcaaatgga atttcagcat ccttaaaaat acctatttga ctgtagaaac
     7081 cttttttgcg tataatatct aaaccttgtc gtgctgctgg aactgcacct gaacattcaa
     7141 caacaacatc tgcaccgtaa ccgtctgtaa ttccattgat atacgttttt aagtctgttt
     7201 gttgtaaatt gactacataa tccatgtgca atgcttctgc tttatctaat ctgactttgt
     7261 cattgtccaa tccagttacc acaacagttg cgcctttact tttcaacact tgtgctacaa
     7321 gtaatccgat tggcccaggt cccattacaa ctgctacatc gccagagttc acttgaatct
     7381 tagaaacgcc atgatgtgca catgctaatg gttctgtcat agctgcagac tgatacgata
     7441 cttcgtctgg aatatgatgc aaactttctt cacgtgcaat gacataatta gtaaatacgc
     7501 catcaacttg tgttccaata ccttttcgat ggttgcataa attatagtct tttgatttac
     7561 agtattcaca ctcattacaa acatagaatg tagtttcaga agtgacacgg tcaccaactt
     7621 taaaatcttt aacgtctgct ccaacttcaa cgatttcacc agaaaattca tgacctaatg
     7681 tcactggaaa attaacttta taatgccctt cataagtatg aatgtctgtg ccacaaattc
     7741 ctgcataatg tactttaatc tttactttat catctagcgg tgttgcaact tctttatcaa
     7801 gaagttctaa gttgccatgt ccttctcttg tttttactaa agctttcacc acaaacacct
     7861 cgatttttaa ttgaatagac taaatagttt aaagataaga tagttaacga tattaccacc
     7921 ttgatcaata cttgaaattt cagatgaacc ttttggcatt tgtacattcg tacctttcgc
     7981 catatctgtg aaaatgggtg ctacgtctgt tgcaatatat aatgaaattg caatcataat
     8041 cgtacccaca atgacagaat gaataatgtt tcctcttgct gcaccaacaa taaacgcgac
     8101 aacaaatggt atagttgcta agtcaccaaa aggtagtact tggtttcctg gtaaaataac
     8161 ggctaataaa acagtgatag gtactaaaat taatgctgtc gaaataaccg ctggatgacc
     8221 taatgctaca gccgcatcca atccaatata aatttcacgt tcgccaaaac gtttatttag
     8281 ccatgttctt gcagactctg aaactggcat taaaccttcc attaagattt ttaccattct
     8341 aggcattaat accattactg cagccattga cattcctaaa ttaatgatgt ctccaggttt
     8401 gtaacctgct aacacaccaa tacctaaacc taaaattaag ccgacaaata tagactctcc
     8461 aaatgcgcca aaacgttttt gaattgtttc aggatcagca tctaacttat tcagaccggg
     8521 tactttttgt aacaatttaa ctaagtaaat acctggtgca taagaaatcg tgcttcctgt
     8581 cgcaatcgat acacctggta aatcaaagaa ctcactcatc atcggtgctg tccaatctgc
     8641 tactttcaaa cagataactt ggaaaataac tgctgctaat aacgcttgcc aaatactgcc
     8701 tgatacggca taaaccattg ctgctgtaaa cgtataatgc caaaaattcc aaatatctac
     8761 attcatcgtc tttgtcactt tagttactag caatacaacg ttaactatga ttccgagtgg
     8821 aataataaat gctgcgacag atgatgccca agcgatagat gatgttgctg gccaacctac
     8881 atcaatcaca ttcagactga cgcctaaatt tttaaccatc gcttgtgctg ctggccctaa
     8941 atttttaact aataaatcga tgactaagaa aatccctaca aaagccacac ctattgttaa
     9001 accagaccta aatgccgctc caattttctg cctaaagaat aggccaagca agaatatgac
     9061 aaccggtaaa ataacagttg cacctaaatc taaaaatccc cttacaaaat cagtgaagta
     9121 actcatattt aaaccctccc tgttatatat gcattgtcac gatactttcc gattgtgatt
     9181 acatttgacg ttacggtcat ttcaacgaca acccttgcta cattcgactg cagtcccttt
     9241 gaattacagt cactgcgttt ctatgtcatc aacaatcatt tgtcgtgata gtcatttata
     9301 tgcaatttgc atatatttat atgttattga cccacgttac atatcaattc cgttattttt
     9361 gtaactctgt taagatttgt tgttttgttt cttcaatacc aataccagtt aagaaattac
     9421 gtgcgttgat aactgggaat ttatattctt tctttgtcat tgcagttgta actaataaat
     9481 ctgcagtgtc ttcataaggt ccaacttctg taattttgat ttgtttaata tctactttaa
     9541 tattgtgttc ctttgccatt tcctcaattg cattatttac tactgttgac gttgcaatac
     9601 ctgcaccaca cgctactaat acttgtttca ttttcaattc ctccaattaa tttttagtta
     9661 tattccaaat aatcattgat tagtgttgct aaaatcgttt catctttcgt tcgcagaatc
     9721 tgctccaatt tttcttcact ttgaaaaatt tgcatcaact gttgtaacaa cttaagttga
     9781 tcatctactt tatccatcgc taacataaaa acgattttca cttctgtctg ttgatcaagt
     9841 gttcccattt caataaacgg cacctctttt tctagaacag ccacacctat cgttctatgg
     9901 ttaatatgtt cgacatctgt atgcggtata gcgaccgaac atagatgtgt tggtaaacca
     9961 gtagcaaatt ctttttctct gtcgatgact gcatctttaa acgttgactt cacgaaccca
    10021 ttttgaaata acacatctga catttgtgac aatacggatt ctttatcagt tgccgacaaa
    10081 ttgagcatta tattttcttt atgcactaat tgctgtccca tccattttcc ctcgcttctt
    10141 tatttgaata attttttaaa atctcattta catcagaatt tttgcgactt tgtatgatgc
    10201 gcttaattgc gtcattgtct tgcgccacat ctctcaattg tagtaacgct cttaagtgtg
    10261 tcactttatc aacagcagca ataggtacaa taatatggat tgctgtgcca tctgacatgt
    10321 atattggttc ttgtaatatc aacatactca tcgctgtttt atgtacatgc ttttcagagt
    10381 atgcatgtgg aatagcaata ttttgcatga taaccatata cgaatcatca aatacctgtt
    10441 gcatctcatg aatatattta atatcaataa aatgattagc aactaacaca tcacttgctt
    10501 tagcaatagc ttcatcaata ttttcaacat gatgcattct tttcacgtgc cttgccggta
    10561 tcaagtcagc taaatctaat gtcttatttt gtgtgacaat cgatccatta atggttgaaa
    10621 ttgaattata attggcaata aaatcttcta aaccatcacg tagttctgta atgtcattaa
    10681 ctgtcgttgt gcgttcaatt aatgccatta acttgtttat ttccttatca atgtcagccg
    10741 attccttatt aatgtacttc atcacttctt tacgtaactt tcgttgctca ttttcagata
    10801 aagctacttt tgtgataaat aattttttat gtgttaggac aaacattggt gaaaagacga
    10861 tgtcataatc taatgtgtaa ttttcaaatg ttctaagtga aatcgcatct aagaaaataa
    10921 tttctggaaa taagtttcgc aactcgtata acatcatttg tgatactgac gtgccttgtg
    10981 tacacacgat aatagctttt atcttgccat cgaagttttc atcttgacgt ctcaaactac
    11041 ctccgaacaa catggttaaa tatgctattt cattatcagg caacgatttt ccgaaatatt
    11101 cagttaacga ttgacatgat tgtttcacca tatgaaataa ggattgataa tttccttgta
    11161 aaggatttat taattcatca cgatccgtta agttatattt aatcctataa aaagcaggcg
    11221 ttaaatgtaa caagagttgc tgtgataatt tctccttatc ttcaatgtta ataaaagtga
    11281 tttgttcaaa atggtgaatc atttgagcga tggccatcgt taaattcgat atgttatctg
    11341 attcctgcaa atcagtccat tgcacacttg ttgaaagtaa gtgtaatgtc aaatataact
    11401 tttccgcttc tggcaaatca ggctcatgtt gcgtcataat ctccgttgct tgatattctt
    11461 tcgtatccct caaatactga taattaatat ttaatggatt catcacatga ccactttgaa
    11521 ttcgtctacg aatcacacaa aggacataag gcaatgaact aagtgatttg tctataaagc
    11581 gactcttcaa aaattgttct acctgtttga tcttgtcttt ttgatatgcg atatcttcga
    11641 atgttaagtt gagcgccttt aaaacttcac ttttagtaat atcatgattc aacctttgat
    11701 caatcaactt aatgaagaaa cggcgaactt caaattcatc accaacaatt tcataaccat
    11761 gctttcgaga atacttaagt gacaaaccat gatcttccaa ttgctctttc acatgattta
    11821 tatcgtgaat gacagtattt ttactgactt gtaaatcaat tgaaaaatgg tttagagaca
    11881 ttgcattttc cttactaaaa agcatgagca ttaaataata acgacgtgtt tctatgctaa
    11941 aaatgacatt gttgccgttt aacatttgct gctccgatac atctcgcttg aataacgtca
    12001 tgatttcaga acttacaata aaatttcctt ggcttgttct ttcaagtttt ggataaccct
    12061 cttgttcaag ccacaaattg attttttgaa tgcgatatcc tagttgtcta cgagacaaac
    12121 caaatatcga ttcaagttct ttaccatgaa tagtaggatt caatacaatt tctctgagta
    12181 tttgctgatt atccccgttc atgttcgatc ctcctttgag catctgagct caatttatca
    12241 tcaatcgtca caccgatgta cacactttga acacatattt ccaaaatgag catgtacatc
    12301 attgtgatgt tttaacaaca tttcaattat atctatattt tttgtgattt taatctttta
    12361 aaataaagca attgaaattt ttacatatat ttttgtgttt tatgtttttt gaagcatttt
    12421 taacatacat atctcaatca ttatcaaatt gtcatgacca atgtaatcca ctataaaaac
    12481 cctaaggacg cttatatcag gcgccttagg gttaactgta tctatttaat taagtattat
    12541 tattcgtatg tacgtaactt atggtctatc aagttccaca cttcttcaac atcaactgct
    12601 gtagcaaaat aagcattggc aggcttacct gtaacatgat ttaaatcgac agccatagtg
    12661 ccataagtta gtggactttg atgttcaatg tcgatattaa cgggtaccat tgtaaacaat
    12721 tctggttgta acaaatacaa aattgtacaa gcatcatgta ttggaccacc atccatatta
    12781 aagtgagtct tgtatgtctt cttaaagaat tgcaataatt ctacgacgaa ctgtgcaaca
    12841 ggattattga tactttcaaa gcgttcaatc acgtgattgt cggctaaaac ttgatgtgtt
    12901 acatctaaac caaacacatt tatagtaatc ccactttcaa aaacacgctt cgctgcttca
    12961 gcatctaccc aaatattgaa ttctgctgta ggcgtccaat ttccaaatgt accaccaccc
    13021 atcaaagtaa tagattcaat atgctcagcg attcttggct cacgaatcaa tgccgttgct
    13081 acattcgtaa gaggacctgt cgctacaatt gttacaggtg tatcactcgt catcactttg
    13141 tttataatca catctgatgc tggcattgca actgcttgac gtgatggtgt cgacggtagt
    13201 ttcggaccat ctaatccaga ttccccatgt atttcagaag caaaggcagc tggtttaatt
    13261 aacggcctat ccgcaccttt cgctactgct atatcttggc gtcccataat atccaatacg
    13321 ttcaaggcgt ttgtcgtatt cttgtcaact gattgattac ctgcgactgt tgttacagct
    13381 aatatctcta gtggactgtc aattgccccc gctaaaatta atgctattgc atcatcgtgt
    13441 cctggatcac aatccataat aatctttctt ttcatttata tatccacctt tcttaagttg
    13501 ttatcgatag cttatgtata tttatttatg tggtgaatca tgtttatttt gaaaaatagt
    13561 tttaactttc tcatattttt ggatacaaac actatttatc tattttatgg cttataaatt
    13621 tatccgatat gccttatcaa cctacctcgc taaaaatagg atgtctacat atctataccg
    13681 acttttgtca actcattttc acaacaatat aaacagcaat ttatatgatt gttacatgat
    13741 tcaaacaatt tttatgaaaa atattttcat acacagaata tatattgata ttaaatttct
    13801 caaaagctat attgagaata attaggaggg atgttgatga aatctttatt tgaaaaagca
    13861 cagcagttcg gcaagtcctt tatgttacct atcgcaatct taccagctgc aggtctattg
    13921 ttgggtatcg gtggtgcatt aagtaatcca aacaccgtta aagcataccc tattttagat
    13981 attaccttat tacaaaatat ttttacatta atgtcagctg caggtagtat tgttttccaa
    14041 aatttaccgg tcatctttgc aattggtgtc gcaatcggat tatctagaag cgataaaggt
    14101 actgcaggtt tagctgcgct gctcggtttc ttaattatga acgcaactat gaatggctta
    14161 ttaactatca cgggcacatt ggcaaaagat cagcttgcac aaaatggaca aggcatggtg
    14221 ctcggtatac aaacggttga aaccggtgtt tttggcggga ttatcacagg tattatgacc
    14281 gcaatacttc acaacaaata tcacaaagtg gtattaccac cgtatttagg tttctttggt
    14341 ggctctagat ttgtccctat tgtcacagca tttgccgcaa tctttttagg tgtattgatg
    14401 tttttcattt ggccaagcat acaagccggc atttatcatg ttggtggatt tgtaacgaaa
    14461 acaggtgcca tcggtacttt tgtttatggc ttcatcttaa gattgttagg tccactcggt
    14521 ttacaccata ttttttactt accgttttgg cagacggcac ttggtggtac tttagaagtc
    14581 aaagggcact tagttcaagg tacgcagaac atcttctttg ctcaacttgg tgatccagat
    14641 gtgacgaagt attattcagg tgtgtcacgc tttatgtcag gccgttttat tacgatgatg
    14701 ttcggcttat gtggtgccgc acttgcaatt tatcacacag ctaaacctga acataaaaaa
    14761 gttgtcggcg gtttaatgtt atccgctgca ctcacttcat ttttaacagg tattaccgaa
    14821 cctttagagt ttagtttctt gtttgtcgca cctattcttt atgtaatcca tgccttcttt
    14881 gatggattag catttatgat ggcagacatt ttcaacatta caattggtca aaccttcagt
    14941 ggaggcttta tcgatttctt actctttggt gtgctacaag gtaatagtaa aacaaactac
    15001 ctatacgtca tacctattgg aattgtgtgg ttctgtttgt attacatcgt tttcagattc
    15061 ttaattacga aatttaattt caaaacacct ggtcgagaag ataaagctgc agcacaacaa
    15121 gttgaggcta ctgaaagagc acaaactatt gttgctggtt tgggaggcaa agataacatt
    15181 gaaatcgttg actgttgtgc aacgagacta cgcgtcacac ttaatcaaaa tgacaaagtc
    15241 gataaagtat tactcgaaag tactggtgcc aaaggtgtaa tccagcaagg cactggtgtg
    15301 caagtaattt atgggcctca cgttacagtt atcaaaaatg aaattgaaga attgctcggg
    15361 gattaagact aaccgaaata tcaacagaac taatggcaac gatgtacgaa gtaagaagtg
    15421 acatcgttgc ttttattttt aatgttacat ttgaagcatt aagttcatca tacactgtag
    15481 tgagcccgca aatcgcctct gctagacaat catcttaatg ctatgattaa agcttaagtg
    15541 ccagatttga atttaatttc aacaacgact ttcactacat taaaaatagg gctactcgac
    15601 acatatagtt gtatcaaata gccctttata caattttttg ggtaaggttt tacaattttt
    15661 ggaatggtat agattttata aaaagttatt taagttcttc tgcttcagcc ataatatctt
    15721 ttaatgtttt agctgaatgt gcgaacttgc tttgttcttc gtcgtttaat gggatttcta
    15781 cgacgttgcg aataccgttt ctattgatga ctgctggaac accaatataa acatcttctt
    15841 catcatattc gccttctaat aatgctgata cagtcaatac ggcatcttca tttctgaaaa
    15901 tcgcttcagt aattctagct aatcccattg caacaccata ataagtggca cctttagctt
    15961 gaataatgtc atatgctgca tcacgtgttt gaacaaaaat ttgttcaatt tgcgctttgc
    16021 cctcaggacg ttgttcaagt aatgtcttca aaggttgacc cgcaatatta gcgtgtgacc
    16081 atactggtaa ttcagtgtca ccatgttcac caataatttg agcatcgacg ctacgtggcg
    16141 caacatcgaa cgcttcgctt aacaataatc taaagcgtgc agagtctaaa attgtaccag
    16201 aacctataac acgttcttta ggtaaaccag agaatttcca tgttgcatac gctaaaatat
    16261 caacaggatt tgtagctacc aagaaaatac catcaaattt tgatgccatt acttcaccaa
    16321 caattgattt gaatattttc aagtttttag atactaaatc taaacgtgtt tctccaggtt
    16381 tttgtgcagc accagcacag atgacaacta gatccgcatc atgacaatca ctgtattcgc
    16441 cagctttcac acgaactgtt gttggagaat atggtgtggc atgttttaaa tccataacat
    16501 ctcctcgaac tttttcagtg tctaaatcaa tgatgactaa ttcatcaaca atgctttggt
    16561 tcactaatga aaatgcgtag cttgaaccta ctgcaccatt acctattaat acaactttgt
    16621 tccctttaaa tttgttcatt acaaaaactc ccttatgatt aattcactaa catacgtgta
    16681 gcttcaaata tgttagttta atgctgctta ttggcgatac aaaagcaaat aaacatctct
    16741 tttattttca acgcataact taaaaggtca tgtgtcatcc gcttttaagt ttgtgattta
    16801 tttcacatat aaaatgtaac atgcattaag tactgggtca atattaaatt gtgatttatt
    16861 tcacatttta ttttaatttt tacacctttt taatttgtat acgattacat cttaggtgtc
    16921 tttagtcttc gtacttcgcc agtgattatt tacactttca catttttatt atcatgttta
    16981 cttttttcta agaaaacaac aatgtttttt gaattagtca aataaatgcg cgcaatcgtc
    17041 ggtgtgcaaa cagacaattg tacacaatgc ttattgataa gtatttaaaa aattaaaaat
    17101 gtcatacaat tatcaaattt gccattttat ttatattttc tcaaaccaat taattgaata
    17161 tcgaaatttt tagtagaata atcaaaatag acagattaaa ggaggagtat catgcttaca
    17221 gaacaagaga aagacattat caaacaaacg gtgcctttac ttaaagagaa agggacagaa
    17281 attacgtcaa tcttttatcc aaaaatgttt aaagcgcatc ctgaactttt aaacatgttt
    17341 aatcaaacga accaaaaacg aggcatgcaa tcttcagcat tagcacaagc tgtaatggcc
    17401 gctgcggtta atatcgataa cttaagtgtt attaaaccag tcattatgcc agtcgcatat
    17461 aaacactgtg cattacaagt ttatgctgaa cattatccaa ttgtggggga aaatttatta
    17521 aaagcaatcc aagacgtgac aggtttagaa gaacatgacc ctgtgattca agcttgggca
    17581 aaagcatatg gtgttattgc ggatgtgttc atccaaattg aaaaagaaat ttatgatcaa
    17641 atgatgtgga ttggttttaa accattcaaa attaccaata ttaaacaaga atctgaagac
    17701 attaaatcat ttacagttga aactgaagaa tatgacttta gtgaatttac accaggccaa
    17761 tacatcacag ttgatgtttc tagtgataaa cttccatata gagctaaacg tcactattct
    17821 atcgtatcag gtgaaaaaaa ccatttaact tttggcgtta aacgtgatgt cacaacagaa
    17881 catgaaggcg aagtttcaac aattttacac gatgagatta aagaaggcga tatgattaat
    17941 ttagctgcgc ctgtaggtgg attcgtatta gagaatacga ctgaaccaca acttttctta
    18001 ggttcaggta ttggcgtcac acctttagta gcaatgtatg aagctgcctc tgccaaaggt
    18061 ttagatacac agatggttca agttgctgaa aatgaacaac atttaccttt caaagacaac
    18121 ttcaacagta tcgcaagcca ttatgacaac gctaaattat atacacactt aaaagataaa
    18181 caaggctata ttggcactga agaattacaa gcatttttag caaataaacc tgaaatttat
    18241 atctgtggtg gtacaaaatt cttacaatct atgatcgaag cacttaaatc tttaaattac
    18301 gatatggatc gcgtacacta cgaaacattt attccaagac taagcgttgc agtataactg
    18361 agaaaagagg tttagtgctc atatgaaact taatcaacgt tacgtaaaag tatttgcatt
    18421 atatttcgta agtattgtta ctgca
//
LOCUS       DANBWH010000031        18376 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.031, whole genome shotgun sequence.
ACCESSION   DANBWH010000031 DANBWH010000000
VERSION     DANBWH010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18376
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.031"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(94..402)
                     /locus_tag="PPY92_000739"
     CDS             complement(94..402)
                     /locus_tag="PPY92_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDJ2624761.1"
                     /translation="MTEHNHDSQLEINNEEELLTLFDEEGNEVLYRKVLEFYHPEFKK
                     EYVILAEEGAQSDEDDMIELVPMINEPDESGDGGKLVPIETDEEWDMIEEVVNTEMEE
                     "
     gene            complement(417..845)
                     /gene="ruvX"
                     /locus_tag="PPY92_000740"
     CDS             complement(417..845)
                     /gene="ruvX"
                     /locus_tag="PPY92_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDJ2624762.1"
                     /translation="MLQHKILGLDVGSRTVGIAISDIMGWTAQGLDTLRINEENNELG
                     IDQLVDIIKKHNVGTVVIGLPKNMNNSIGFRGEASLTYKEKLLEAYPSIEIVMWDERL
                     STMAAERSLLEADVSRQKRKQVIDKMAAVFILQGYLDSLH"
     gene            complement(849..1109)
                     /locus_tag="PPY92_000741"
     CDS             complement(849..1109)
                     /locus_tag="PPY92_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDJ2624763.1"
                     /translation="MENFDKTMKFDYEELPTQDVRDVLNNVYRTLDERGYNAVNQIVG
                     YLLSGDPAYIPRQNEARNQIRHIDRDVIMEELVSYYLKEQNK"
     gene            complement(1172..3802)
                     /gene="alaS"
                     /locus_tag="PPY92_000742"
     CDS             complement(1172..3802)
                     /gene="alaS"
                     /locus_tag="PPY92_000742"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDJ2624764.1"
                     /translation="MKKLKASEIRQKYLDFFVEKGHMVEPSAPLVPIDDDTLLWINSG
                     VATLKKYFDGRETPKKPRIVNSQKAIRTNDIENVGFTARHHTFFEMLGNFSIGDYFKQ
                     EAIEFAWEFLTSDKWMGMEPDKLYVTIHPEDMEAYNIWHKDIGLEESRIIRIEGNFWD
                     IGEGPSGPNTEIFYDRGEAYGQDDPAEEMYPGGENERYLEVWNLVFSEFNHNKDHSYT
                     PLPNKNIDTGMGLERMASVSQNVRTNYETDLFMPIMNEIEKVSGKQYLVNNEQDVAFK
                     VIADHIRTIAFAISDGALPANEGRGYVLRRLLRRAVRFSQTLGINEPFMYKLVDIVAD
                     IMEPYYPNVKEKADFIKRVIKSEEERFHETLEDGLAILNELIKKAKATTNEINGKDAF
                     KLYDTYGFPIELTEEIAVQAGLKVDMTTFESEMQQQRDRARQARQNSQSMQVQSEVLK
                     NITSASTFVGYDTATAQTTLTHLIYNGEEVSQVEAGETVYFMLTETPFYAVSGGQVAD
                     TGIVYNDNFEIAVSEVTKAPNGQNLHKGVVQFGQVNVGATVSAEVNQNDRRDIQKNHS
                     ATHLLHAALKSVLGDHVNQAGSLVEADRLRFDFSHFGPMTNDEIDQVERLVNEEIWKG
                     IDVNIQEMDIASAKEMGAMALFGEKYGDVVRVVNMAPFSIELCGGIHVRNTSEIGLFK
                     IVSESGTGAGVRRIEALTGKAAFLYLEDIQEKFNTMKSQMKVKSDDQVVEKLTQLQDE
                     EKALLKQLEQRDKEITSLKMGNIEDQVEEINGYKVLVTEVDVPNAKAIRSTMDDFKSK
                     LQDTIIILASNVDDKVSMVATVPKSLTNNVKAGDLIKQMAPIVGGKGGGRPDMAQGGG
                     TQPENISKSLSFIKDYIKNL"
     gene            complement(4145..6622)
                     /locus_tag="PPY92_000743"
     CDS             complement(4145..6622)
                     /locus_tag="PPY92_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDJ2624765.1"
                     /translation="MSDPTLFDYSMIKGTVEAILFQNSDNFYTVLKVDTIETNEDFDT
                     MPTVVGFLPNIVEGDVYTFKGQVVDHPRYGKQLKAETFEKEMPQTKEAIISYLSSDLF
                     KGVGKKTAQNIVNTLGDNAINDILDDHSVLEKVSGLSKKKQKQIAEQISANQESEKIM
                     IRLHDLGFGPKLSMAIYQFYLGDTLTILDRNPYQLIYDIKGIGFNKADQLARNIGIAY
                     NDNERLKAALLYTLEEECIKQGHTYLPINVVIDLTVDVLNYQDEEVIEPEKLDEMLQY
                     LNEEKRLIIDNEQVAIPSLYYSEIKSVQNLFRIKTHTNKLTEIEQSDLQMHIGEIEDA
                     NQVNYAASQKEALQTAINSKVMLLTGGPGTGKTTVIKGIVELYAEIHGLSLDYDDYVN
                     DDYPVVLAAPTGRASKRLQESTGLEAMTIHRLIGWNQDTKPEDILENEINARLIIIDE
                     MSMVDTWLFHQFLSAVPLDAQLIFVGDEDQLPSVGPGQVFKDLIESKAIPRVNLTEVY
                     RQQDGSSIIELAHRMKLGQKIDITQRFHDRSFINCQANQIPTVVEKVVTSAVNKGYTM
                     ADIQVLAPMYKGNAGIKRLNQVLQDILNPKKKDTREIEFGDVVFRKGDKVLQLVNRPN
                     DNIFNGDIGVIVGIFWAKENALNKDVLVVDFEGNEITFTKQDMMELTHAYCTSIHKSQ
                     GSEFPIVIMPIVKQYFRMLQRPILYTGLTRAKTSLVLLGDPEAFDIGLKTNGQARLTQ
                     LCTLLKNYFNSEDEEMLENTATNDTGASQTTIDDQVEAPMSSNDSGEVTSDSTKTDIN
                     VLTEATMFKIDPMINMGEITPYDFIER"
     gene            complement(6624..7292)
                     /locus_tag="PPY92_000744"
     CDS             complement(6624..7292)
                     /locus_tag="PPY92_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDJ2624766.1"
                     /translation="MIDQQTIYQYIQNGKIEEALQALFGNIEEDPTIIENYINAGIVL
                     ADANEIEKAERFFQKALTIDPKNGVVFYNLANVYYNQQRYQEAIKLYQQALQTEIEQV
                     DCNYMIGMAFNQLESFKLALPYLMTAAELDKDKDAEVQFQYGLVLCQLEMFNEAITQL
                     KHVLTIDKNHVDARYNLGLALFMKNEDIDEAITHFKEAVTIDPKHLLSQHALKTFTKM
                     KEEE"
     gene            complement(7987..9105)
                     /gene="mnmA"
                     /locus_tag="PPY92_000745"
     CDS             complement(7987..9105)
                     /gene="mnmA"
                     /locus_tag="PPY92_000745"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDJ2624767.1"
                     /translation="MSNKDIRVVVGMSGGVDSSVTAHVLKEQGYDVIGIFMKNWDDTD
                     ENGVCTATEDYNDVIEVCNQIGIPYYAVNFEKEYWDKVFTYFLDEYKKGRTPNPDVMC
                     NKEIKFKAFLDHAMNLGADYVATGHYARIHRHEDGHVEMLRGVDNNKDQTYFLNQLSQ
                     QQLSKVMFPIGDIEKSEVRRIAEEQGLVTAKKKDSTGICFIGEKNFKTFLSQYLPAQP
                     GDMITLDGKKMGKHSGLMYYTIGQRHGLGIGGDGDPWFVVGKNLKDNVLYVEQGFHHD
                     ALYSDYLIASDYSFVNPEDNDLDQGFECTAKFRYRQKDTKVFVKRENDHALRVTFAEP
                     VRAITPGQAVVFYQGDVCLGGATIDDVFKNEGQLNYVV"
     gene            complement(9106..10248)
                     /locus_tag="PPY92_000746"
     CDS             complement(9106..10248)
                     /locus_tag="PPY92_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDJ2624768.1"
                     /translation="MEIYADYAATTPVKPEVVDAMMMIYNSHYGNPSSIHAKGRDARK
                     YLDESRRQIAQLLGADTHEIIFTSGATESNNTAIKGIVKANEQLGNHIITSKIEHHSV
                     LHVFEQLEREGFDVTYLDVDDTGAIDLDQLEETITDKTILVSIMFVNNEVGTVQQIYD
                     IQDIIAETNAYFHVDAVQAIGHLDVKFDEFEIDAMSITAHKFGGPKGVGALLVKDHVT
                     LDYPQLGGEQELKRRAGTENLAQIVGMAKALQLAEKNRDDNNIHLMNLKEQFLVKLQE
                     RAIPFELNGSMTDATGHIVNLYFPFVEVEMMLTLLDMAQIYVSSGSACTAGSTQPSHV
                     LDAMFEDEERSNHSIRFSFNELTTENEINAIVAEIHKIYFKFKEES"
     gene            10560..11573
                     /locus_tag="PPY92_000747"
     CDS             10560..11573
                     /locus_tag="PPY92_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDJ2624769.1"
                     /translation="MAIKYSALNLVPIREGDDERTAINDMVKLAQHLDELSYERYWIA
                     EHHNAPNLVSSATALLIQHTLEHTKHIRVGSGGIMLPNHAPLIVAEQFGTMATLFPNR
                     VDLGLGRAPGTDMMTASALRRDQHDGVYKFPEEVSLLQQYFGPAHQQAYVRAYPAVGK
                     NVPLYILGSSTDSAHLAARKGLPYVFAGHFAPQQMKEAIEIYKTLFEPSDVLDEPYVI
                     VCLNTIVAENDDEAQYLASSMAQVMVSITRGRMQPVQPPTHDLQNILTPREYAMAMER
                     QKISLIGSENTVQQKIQDFMETYGEVNEIMAISYIYDKDMQLDSYRRFKNVINQINEK
                     NTL"
     gene            complement(11810..11956)
                     /locus_tag="PPY92_000748"
     CDS             complement(11810..11956)
                     /locus_tag="PPY92_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624770.1"
                     /translation="MSFMDKAKDAVEKFKNSDNEQVKNVKDKINEYTGSNNEEKKENE
                     DKEK"
     gene            complement(11996..12178)
                     /locus_tag="PPY92_000749"
     CDS             complement(11996..12178)
                     /locus_tag="PPY92_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDJ2624771.1"
                     /translation="MADESKFDQFKGNVKETVGNVTDNKELEKEGQQDKATGKAKEVV
                     ENAKNKITDAIDKLKK"
     gene            complement(12278..12700)
                     /locus_tag="PPY92_000750"
     CDS             complement(12278..12700)
                     /locus_tag="PPY92_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDJ2624772.1"
                     /translation="MKISTKGRYGLTLMISLAKKEGQGCISLKSIAEENNLSDLYLEQ
                     LVGPLRNAGLIRSVRGAKGGYQLRVPAEEISAGDIIRLLEGPITFVESIESEPPAQKQ
                     LWIRMRDAVRDVLDNTTLKYLAEYVDTSEDLDGYMFYI"
     gene            12785..14059
                     /locus_tag="PPY92_000751"
     CDS             12785..14059
                     /locus_tag="PPY92_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDJ2624773.1"
                     /translation="MSTEPLASRMRPKNIDEIISQQHLVGPKGIIRRMVDTKKLTSMI
                     FYGPPGIGKTSIAKAISGSTQYKFRQLNAVTNTKKDMQLVVEEAKMSGQVILLLDEIH
                     RLDKAKQDFLLPHLENGKIVLIGATTSNPYHAINPAIRSRAQIFELYPLNDEDVRQAL
                     TRAIEDDENGLKTYQPKIDEDAMTYFSTQSQGDVRSALNALELAVLSADNDKDGYRHV
                     TLQDAKDCLQKGAFVSDKDGDMHYDVMSAFQKSIRGSDVNAALHYLARLIEAGDLPTI
                     VRRLLVISYEDIGLASPNAGQRTLAAIESAERLGLPEARIPLSQAVIELCLSPKSNSA
                     MSAIDSALSDIRNGHVGQIPNHLKDGHYQGAKDLGRSIGYKYPHQYVNGYVSQQYLPD
                     KLKNKIYYEPKTTSKSEQQLKEIYNNLLKQRP"
     gene            complement(14220..14993)
                     /locus_tag="PPY92_000752"
     CDS             complement(14220..14993)
                     /locus_tag="PPY92_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HDJ2624774.1"
                     /translation="MKHQFSRNELAIGQQGLDLLKQQTVVILGVGGVGSFAAEALART
                     NIGHIILIDKDDVDITNVNRQLHALTTTVGQSKVSLMEERIKLINPDCKVTPLHMFYT
                     EETYEEIFDNYDIDYFVDASDTIMYKVHLMKECLDRGIKVISSMGAANKTDPTRFTIS
                     DISKTHTDPMAKIIRQKLKKLGIRKGIPVVFSDESPIVIREDVKDIVGDKNAINRKGQ
                     MPPSSNAFVPSVVGLICASYVVNDVLKDIPVRRIKDKGQ"
     gene            complement(14993..15121)
                     /locus_tag="PPY92_000753"
     CDS             complement(14993..15121)
                     /locus_tag="PPY92_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624775.1"
                     /translation="MGFIAFNKCFLVEFWENNQSDNAKYKILSYYVLMYKSVKEVK"
     gene            complement(15186..15376)
                     /gene="ssrS"
                     /locus_tag="PPY92_000754"
     ncRNA           complement(15186..15376)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PPY92_000754"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(15454..17220)
                     /gene="aspS"
                     /locus_tag="PPY92_000755"
     CDS             complement(15454..17220)
                     /gene="aspS"
                     /locus_tag="PPY92_000755"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDJ2624776.1"
                     /translation="MSKRTTYCGLVTEAFLGQEITLKGWVNNRRDLGGLIFVDLRDRE
                     GIVQVVFNPAFSEEALKIAETVRSEYVVEVQGTVTKRDPETVNPKIKTGQVEVQVTNI
                     KVINKSETPPFSINEENVNVDENIRLKYRYLDLRRQELAQTFKMRHQITRSIRQYLDD
                     EGFFDIETPVLTKSTPEGARDYLVPSRVHDGEFYALPQSPQLFKQLLMISGFDKYYQI
                     VKCFRDEDLRADRQPEFTQVDIEMSFVDQEDVMQMGEEMLKKVVKEVKGVEINGAFPR
                     MTYKEAMRRYGSDKPDTRFEMELIDVSQLGRDMDFKVFKDTVENDGEIKAIVAKGAAE
                     QYTRKDMDALTEFVNIYGAKGLAWVKVVEDGLTGPIGRFFETENVETLLTLTGAEAGD
                     LVMFVADKPNVVAQSLGALRVKLAKELGLIDETKLNFLWVTDWPLLEYDEDAKRYVAA
                     HHPFTSPKEADIAKLGTAPEEAEANAYDIVLNGYELGGGSIRIHDGELQEKMFEVLGF
                     TKEQAQEQFGFLLDAFKYGAPPHGGIALGLDRLVMLLTNRTNLRDTIAFPKTASATCL
                     LTNAPGEVSDKQLEELSLRIRH"
     gene            complement(17236..>18376)
                     /locus_tag="PPY92_000756"
     CDS             complement(17236..>18376)
                     /locus_tag="PPY92_000756"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDJ2624777.1"
                     /translation="IFESTDLFARGVGDSTDVVQKEMYTFKDKGDRSITLRPEGTAAV
                     VRSYIEHKMQGNPNQPIKLYYNGPMFRYERKQKGRYRQFNQFGVEAIGAENPSVDAEV
                     LAMVMHIYQSFGLKHLKLVINSVGDMASRKEYNEALVKHFEPVIHEFCSDCQSRLHTN
                     PMRILDCKVDRDKEAIKTAPRITDFLNEESKAYYEQVKAYLDDLGIPYIEDPNLVRGL
                     DYYTHTAFELMMDNPNYDGAITTLCGGGRYNGLLELLDGPSETGIGFALSIERLLLAL
                     EEEGIELDIEENLDLFIVTMGDQADRYAVKLLNHLRHNGIKADKDYLQRKIKGQMKQA
                     DRLGAKFTIVIGDQELENNKIDVKNMTTGESETIELDALVEYFKK"
BASE COUNT         5632 a         3568 c         2614 g         6562 t
ORIGIN      
        1 aaattataag cgttaattat ttatacattg cttgacttaa aaaaatactc ttgcctaccc
       61 atcttaaggt tagcaagagt aaaatctttt taattattct tccatttcag tatttacaac
      121 ttcttcaatc atgtcccatt cttcatcagt ttcgattggt actaacttac caccgtcacc
      181 tgactcatct ggttcattga tcattggtac aagctcaatc atatcgtctt catctgattg
      241 agcaccttct tcagctaaga taacatactc ttttttgaat tcaggatgat aaaattctaa
      301 aacttttcgg tataaaactt catttccctc ttcatcgaat aaagttaata attcttcttc
      361 gttattaatt tctagttgtg aatcatgatt atgttcagtc atagtaaaat ctccttttaa
      421 tgtagtgaat ctaaatagcc ttgtaaaata aataccgctg ccattttatc aatcacttgt
      481 tttctttttt gtcttgaaac atctgcttct aataatgatc gttcagcagc cattgtgctt
      541 aatctttcat cccacatcac aatctcaata gaaggataag cttctaataa tttttcttta
      601 tatgttaacg aagcttcgcc tcgaaatcct attgaattat tcatgttttt aggtagtcct
      661 attacgactg tacccacatt atgtttttta ataatgtcta ctaattggtc aatacctaat
      721 tcattatttt cttcattgat tcggagtgtg tctaatcctt gtgccgtcca acccattata
      781 tcactaattg caattcctac cgttctacta ccgacatcga gtcctaaaat tttatgttgt
      841 aacataaatt atttattttg ctcttttaaa tagtaagaaa caagttcttc cataataaca
      901 tctctatcaa tatgacgaat ttgatttctt gcttcattct ggcgtggaat atacgcaggg
      961 tcacctgata ataaataacc tacaatttgg tttacggcat tatatcctcg ttcatctaat
     1021 gttcgataaa cattatttaa aacatctctt acatcttgcg ttggaagttc ttcatagtcg
     1081 aatttcattg ttttatcaaa gttttccatt tgcgacactc ctttaattac aaatataact
     1141 cactatcatc atacaatatt atggctttaa attatagatt tttaatgtaa tctttaataa
     1201 agcttaatga ttttgagata ttttcaggtt gtgtaccgcc accttgagcc atatctggac
     1261 gaccgccacc tttaccacca acgattggtg ccatttgttt gataagatca ccggctttaa
     1321 cgttatttgt taaagattta gggacagttg caaccatcga tactttatca tcaacattac
     1381 ttgcaagaat gataattgta tcttgtagtt tagatttaaa atcatccatt gtcgagcgaa
     1441 ttgctttcgc atttggtaca tccacttcag taaccaatac tttatagcca ttgatttctt
     1501 caacttgatc ttcaatatta cccattttaa gtgatgtgat ttctttgtca cgttgctcta
     1561 attgttttaa taatgctttt tcttcatctt gtaattgtgt taacttttcg actacttgat
     1621 catcagattt cactttcatc tgtgatttca tcgtattaaa tttctcttga atatcttcta
     1681 aatataagaa agctgcttta cctgttaatg cttcaatacg acgcacacca gctcctgtac
     1741 ctgactcact tactattttg aataagccaa tttcagaagt attgcggaca tgaataccac
     1801 cacataattc aattgaaaat ggtgccatat ttactacacg cacaacatca ccatattttt
     1861 caccgaataa tgccattgcg cccatttctt tagctgaagc aatatccatt tcttgaatgt
     1921 taacgtcaat acctttccaa atttcttcat ttactaagcg ttcaacttga tcaatttcat
     1981 cattagtcat tggaccaaaa tgagagaaat caaaacgtaa acgatctgct tctactagtg
     2041 aaccagcttg gttaacatga tcacccagta ctgatttcaa cgctgcatgt aataaatgtg
     2101 ttgcactatg gttcttttga atgtcacgtc gatcattttg gttcacttca gcagacactg
     2161 tagcgccaac atttacttgg ccaaattgta ctactccttt atgcaagttt tgaccatttg
     2221 gtgctttggt tacttcacta acagcaattt caaaattgtc attataaaca atacctgtat
     2281 ccgcaacttg tccaccactg actgcataaa atggtgtttc cgttaacatg aagtatactg
     2341 tttcacccgc ttcaacttgt gaaacttctt caccattata tatcaagtgt gttagtgttg
     2401 tttgagctgt cgcagtatca taaccaacaa aagtacttgc agatgtaata tttttcaata
     2461 cttcactttg aacttgcatt gattgagaat tttgacgtgc ttgacgtgca cgatcacgtt
     2521 gttgttgcat ttctgactca aatgttgtca tatcaacttt caatcctgct tgcactgcta
     2581 tttcttcagt taattcaatt gggaacccgt acgtatcata caatttaaat gcatctttcc
     2641 cattaatttc atttgttgtc gctttagctt ttttaattaa ttcatttaaa atcgctaaac
     2701 catcttctaa tgtttcatgg aatcgttctt cttcagactt tataacacgc ttaatgaaat
     2761 ctgctttttc cttaacattt ggataatatg gttccataat gtctgcaaca atatcaacaa
     2821 gtttgtacat aaatggctca ttgattccta acgtttgact aaaacgaacg gcacgacgta
     2881 acaatcgacg taatacatat cctctacctt cattggcagg taatgcacca tcagaaattg
     2941 caaatgcaat cgtacgaatg tggtcagcaa ttactttaaa tgccacatct tgttcgttgt
     3001 ttactaaata ttgtttacct gatacttttt cgatttcatt cattataggc ataaataaat
     3061 ctgtttcata gttagtacgt acattttgag aaactgaggc catacgctca agccccatgc
     3121 cggtatcaat atttttatta ggtaatggtg tgtaactatg atctttatta tgattgaatt
     3181 cactaaatac taagttccat acttcaagat agcgttcatt ttctccacct ggatacattt
     3241 cttctgccgg atcgtcttgt ccatatgctt ctccgcgatc atagaaaatc tcagtgttcg
     3301 gtcctgaagg cccttcacca atatcccaga agttaccttc aatgcgaata atacgacttt
     3361 cttcaagccc aatatcttta tgccaaatgt tatatgcttc catatcttcc ggatgaatcg
     3421 taacgtacaa tttatctggc tccataccca tccatttatc actcgttaaa aattcccaag
     3481 caaattcaat cgcttcttgt ttaaaataat caccaattga gaagttacct aacatttcaa
     3541 agaatgtatg gtgacgcgct gtgaaaccaa cattttcaat atcatttgta cgaatagctt
     3601 tttgagagtt tacaattctt ggctttttag gtgtttcacg tccatcaaaa tatttcttta
     3661 atgttgctac acctgaatta atccataata atgtatcatc atcaattggc actaatggtg
     3721 cagaaggttc aaccatatgt cctttttcaa caaagaaatc tagatatttt tgtctaattt
     3781 cactcgcttt taactttttc atcttttaca catcctattt actgttttta aattaccatt
     3841 ccataaaaat tgatgacaca gatagtcgat ttgaaaaact agtataaatc aatatcattt
     3901 tttattatta aaaaataaaa aacgcccatc ctcaaaaggg acgaacgtta tcgcggtacc
     3961 accctagtta taaatgcaat tcaacacatt tatcacttta aatcgactat acagttgtgc
     4021 ataaagtagc gttcactaat gtttgttgta cttttcacca accagtacat ctctgataaa
     4081 caaatcatta actactcatc tttatacgaa tttaattcta ttttagttac atttacgctt
     4141 gttgtcaacg ttctataaag tcatacggcg tgatttctcc catattaatc attgggtcaa
     4201 ttttaaacat tgtagcttcc gttaatacat ttatatctgt ttttgttgaa tcagacgtaa
     4261 cttctccact atcattcgat gacattggcg cttctacttg atcatctatt gtcgtttgtg
     4321 aagctcctgt atcattagtt gctgtgtttt ccagcatttc ttcatcttct gaattaaaat
     4381 aatttttcaa caatgtacat aattgtgtta aacgcgcttg accatttgtt tttaatccaa
     4441 tatcaaatgc ttctggatca ccaagtagaa ctaaactcgt tttcgctcta gttaaaccag
     4501 tatataatat cggtctttgt aacattctaa aatactgttt aacaataggc atgataacaa
     4561 taggaaattc tgaaccttgt gatttatgga ttgatgtaca ataagcatgt gttaattcca
     4621 tcatatcttg tttcgtaaat gtaatttcat taccttcaaa atccacaaca agtacatctt
     4681 tattaagggc attttctttc gcccaaaaaa taccaacaat aactcctatg tcaccattga
     4741 atatgttatc atttggccta ttaacaagtt gtaatacttt gtcacctttt ctaaagacta
     4801 catcaccaaa ctcaatttct cgtgtgtctt tctttttagg gtttaaaata tcttgtaaaa
     4861 cttgatttaa acgtttaata ccggcatttc ctttatacat tggtgcaagc acttgaatat
     4921 cagccatagt atacccttta ttaacagcac tagtaactac cttctcaaca actgttggta
     4981 tttggtttgc ctgacagtta ataaaacttc tatcatgaaa acgctgtgta atatcaattt
     5041 tctgacccaa tttcattcga tgtgctaatt ctataatgct tgaaccatct tgttgacgat
     5101 atacttcagt cagattaact cgtggtatag ctttcgattc aattaaatct ttaaatactt
     5161 gaccaggacc tacagaaggc aattggtcct catcacctac aaatatcaat tgtgcatcta
     5221 aaggaactgc acttaaaaat tggtgaaaca accaagtatc taccatagac atctcatcaa
     5281 tgattatgag tcgtgcgttt atttcatttt ctaatatatc ctctggcttt gtgtcttgat
     5341 tccaacctat taaacgatga atcgtcattg cttctaatcc agttgactct tgtagtctct
     5401 tagacgctct tcctgttggc gctgctaata caactggata atcatcattg acataatcat
     5461 cataatctaa tgataagcca tgaatctcag catataattc tacaatacct ttaattactg
     5521 tcgtctttcc tgttcccggt ccaccggtta atagcatcac cttagaattg atagccgttt
     5581 gcaaagcttc tttttgtgaa gctgcatagt tcacttgatt cgcatcttct atttcaccaa
     5641 tatgcatttg taaatctgac tgttcaattt ccgtaagttt atttgtatgc gtctttattc
     5701 tgaataagtt ttgaacactt ttgatttcag aataatacaa acttggaatt gcaacttgtt
     5761 cattgtcaat aattagtcgt ttttcctcat ttaagtattg caacatttcg tctaattttt
     5821 caggttcgat gacctcttca tcttgataat ttaatacatc aaccgttaaa tctataacaa
     5881 cattgatagg caaatatgta tgtccctgtt taatacattc ttcttctaac gtatagagca
     5941 acgcagcttt taatcgttca ttatcgttat aagcgatacc aatatttcta gcaagttgat
     6001 ctgctttatt aaaaccaata cctttaatat cataaatcaa ttgatatgga tttcgatcta
     6061 aaatagtcag tgtatcgccg agataaaact gataaattgc cattgaaagt ttaggaccaa
     6121 accctaaatc atgtaaacga atcattattt tttcagattc ttgatttgct gaaatttgtt
     6181 ctgcaatttg tttctgtttc tttttagata atcccgaaac tttttctagc actgaatggt
     6241 catctaatat atcatttatc gcattgtcac ctaatgtatt aacaatattt tgagctgtct
     6301 ttttacctac acctttaaac aaatcactag ataaataact tataattgct tctttcgttt
     6361 gtggcatttc tttttcaaaa gtctctgctt ttaattgttt accataacgt ggatgatcaa
     6421 caacttgccc tttaaatgtg tagacatcgc cttcaacaat attcggaaga aaccctacaa
     6481 cagttggcat tgtatcaaag tcttcatttg tttcaatagt atctacttta agcactgtat
     6541 aaaaattatc actgttttga aacaatatcg cttcaacagt acctttgatc attgaataat
     6601 caaatagtgt agggtctgac atgttactcc tcctctttca ttttagtgaa tgttttcagc
     6661 gcatgctgac ttaataagtg tttagggtcg atagtcacag cttctttaaa atgagttatt
     6721 gcttcatcaa tatcttcatt tttcataaat aacgctaagc ccaaattgta tcttgcatca
     6781 acatgatttt tatcaatcgt taatacatgt ttaagttgag ttatggcttc attaaacatt
     6841 tctaattgac ataatacaag accatattga aattgaactt ctgcatcttt gtctttatct
     6901 agttccgcag cagtcattaa atacggcaat gccagcttaa atgattctaa ctgattaaac
     6961 gccataccga tcatataatt acaatcaact tgttcaatct ctgtttgtaa tgcttgttga
     7021 tataatttaa tagcttcttg ataacgttgc tgattataat atacatttgc tagattataa
     7081 aatacgacgc cattcttcgg atctattgtt aaagcttttt ggaaaaaacg ctctgccttt
     7141 tcaatctcat tcgcatcagc aagtacgata ccagcattaa tataattttc aataattgta
     7201 ggatcttctt cgatatttcc gaacaatgct tgtaacgctt cttctatttt tccattttgt
     7261 atgtattgat aaattgtttg ttgatctatc atttacgaac ctcatttctc atcaattata
     7321 acatcttgat aaattgtatg tctcgaatca cttaacaacg aataaaatat aatctaatat
     7381 cattttcatt catgaaaaag cgggaatgga atagaaatgc taaagaacca ttaacggttt
     7441 attatgtaat ggttcttcca cattagccac cactattatg tacttaaaaa taggaataca
     7501 taattagatt catgcatagg gagtgggaca gaaatgatat tttaacaaaa ttaaattcgt
     7561 tatccccaac tggcattgcc tgtagaattt ctttacgaaa ttctctgtgt tggggcccca
     7621 tccccaactt gcacattatt gaaagctgac ttttggtcag cttctatgtt gggggccccg
     7681 tccccaactt ccattgcctg ttgaaattag ggttccaatt tctctgtgtt ggggccccgc
     7741 caacttccat tgcctgttga aattggggtt ccaatttctc tatgttgggg ccccgtcccc
     7801 aacttccatt gcctgtagaa tttctttacg aaattctcta tgttggggcc ccgccaatat
     7861 aacattgtag agcctaggac attgtgatgt cccagactct atcatcatga attattctca
     7921 tcaaaaactg tctttcgtca ttttcgacgt tgaaacttca aataagtaat ttattgttgc
     7981 cattgtttat acaacataat ttaattgacc ttcatttttg aacacatcgt caattgttgc
     8041 accaccaaga cacacatcac cttgataaaa aacaactgct tgtccaggtg tgattgctct
     8101 tactggctca gcaaaagtaa cacgtagtgc atggtcgttt tcacgtttca caaaaacttt
     8161 cgtatctttt tggcgatatc taaatttagc tgtacattca aaaccttgat ctaagtcatt
     8221 atcttctgga tttacaaatg aatagtctga agcaattaag taatcactgt ataatgcatc
     8281 gtgatggaat ccttgttcta catataaaac attatctttt aggtttttac cgacaacaaa
     8341 ccaaggatcg ccatctccac ctatacctaa tccatgtctt tgtcctattg tgtaatacat
     8401 caaaccacta tgtttaccca ttttcttacc atcaagtgtt atcatatcac ccggttgtgc
     8461 aggtaaatat tgtgataaaa atgttttaaa gtttttttcg ccgataaaac aaatgcctgt
     8521 agaatctttt ttcttagcag taacaagtcc ttgttcttca gcaattcgac gcacttcact
     8581 cttttcgatg tcgccaattg ggaacatcac ttttgaaagt tgttgttgag ataattgatt
     8641 caagaagtat gtttgatctt tattattatc tacaccacgt aacatttcaa catgaccatc
     8701 ttcatgacga tgtatgcgtg cgtaatgtcc tgttgctaca taatctgcac ctaaattcat
     8761 cgcatgatct aaaaaggctt taaacttaat ttctttatta cacataacgt ctggatttgg
     8821 agtacgacct tttttgtatt catctaagaa atacgtaaag actttatccc aatattcttt
     8881 ttcaaaatta acagcgtaat atggaatgcc aatttgatta cacacttcaa taacatcgtt
     8941 gtaatcttca gttgcagtac atacgccatt ttcgtcagtg tcatcccagt ttttcataaa
     9001 tatgccaatg acatcataac cttgttcttt taagacgtgg gctgttacag aactatctac
     9061 accgcctgac ataccaacga caacacgtat atctttattt gacaattatg actcctcctt
     9121 aaatttaaaa tatattttat gaatttcagc tacaattgca ttaatttcat tttcagtagt
     9181 caattcgtta aaactaaatc gaatcgaatg atttgatcgc tcctcatctt cgaacattgc
     9241 atctaaaaca tgcgacggtt gtgtagagcc tgctgtacat gcagatccag acgacacata
     9301 gatttgtgcc atatccaaca atgttaacat catttcaact tcaacaaacg gaaaatatag
     9361 atttacaata tggcctgtag catccgtcat tgaaccattt aattcaaatg gaatcgctct
     9421 ttcttgtaat ttaactaaaa attgttcttt taaattcatt aaatgaatat tgttatcgtc
     9481 tcgattcttt tctgctaatt gtaatgcttt agccatcccg acaatttgtg caagattttc
     9541 agtacctgca cggcgtttca attcttgttc accgccaagt tgaggataat ctaatgtgac
     9601 atggtcttta actaataatg caccgacacc ttttggtccg ccaaacttat gtgcagtaat
     9661 actcattgcg tcgatctcaa attcgtcaaa cttaacatca agatgtccaa ttgcttgaac
     9721 cgcatcaaca tggaaatatg catttgtctc agcaataata tcttgaatat cataaatttg
     9781 ttgcactgtg ccaacttcat tatttacaaa cataatagat actaaaatcg tcttatctgt
     9841 aattgtttct tcaagttgat ctaaatcaat agcacctgta tcatcaacat ctagatatgt
     9901 tacatcaaaa ccttctcgct ctaattgttc aaaaacatgt aacacagaat gatgttcaat
     9961 cttcgatgtg ataatgtgat tacccaattg ttcatttgct tttactatgc ctttaattgc
    10021 cgtattattc gattctgttg caccactcgt aaatataatt tcatgtgtat ctgcaccaag
    10081 taattgtgca atttgacgtc ttgactcatc taaatattta cgcgcatctc ttcccttagc
    10141 atgtattgat gatggattac cataatgcga attgtaaatc atcatcatcg catctactac
    10201 ttcaggtttt actggtgtgg tcgcagcata atctgcataa atttccatgt ttggacactc
    10261 ctcacaattt tatcaatgtt ccaataatag caccttacat actatttttc tacttttctg
    10321 tttaacttta tttataatgt ttttaattat attttaccat tttctacaca tgcttttcga
    10381 taggcttttt taagtttatc gctttattct tgtctttttt ataaatttta gtatttgcag
    10441 atattttttc atttgtaaaa tgtaacgtac tattattttg gttatgagca atttaatatt
    10501 tatctggtta ttcgattggt atacttctta tatcataaaa aaggaaggac gatataaaaa
    10561 tggcgattaa atattcagca ttaaaccttg tccctattcg agaaggtgac gatgaacgaa
    10621 cagcaattaa tgatatggtt aagctcgcac aacatttaga tgaattatcg tatgaaagat
    10681 attggattgc tgaacaccat aacgctccca acctagtaag ttcagcaact gctttattga
    10741 ttcaacatac gttagaacat acgaaacaca tacgtgtagg ttctggagga atcatgttac
    10801 ctaaccatgc tccattaatc gttgcggaac aattcggcac gatggcaaca ttatttccaa
    10861 atcgtgtcga tttaggatta ggacgtgcac ctggaacaga tatgatgacc gcaagtgcat
    10921 taagacgaga tcaacatgat ggtgtttata aatttccaga agaagtttca ttattacaac
    10981 aatatttcgg cccagctcac caacaagcat atgttcgtgc ttatccagca gtaggtaaaa
    11041 atgtgccttt atacattctt ggttcttcaa cagattctgc acatttagct gctcgcaaag
    11101 ggcttccata tgtgttcgct ggacattttg cacctcaaca aatgaaagaa gctatcgaaa
    11161 tttacaaaac gttatttgaa ccttctgatg tattagacga accttatgtt attgtatgtt
    11221 taaatacaat cgttgctgaa aatgatgacg aagcacaata tttagcttca tctatggcac
    11281 aagtaatggt tagtatcact cgtggcagaa tgcagcccgt tcaaccgcca acacatgatc
    11341 tacaaaatat attaacgccg agagaatatg cgatggctat ggaaagacag aaaatatcat
    11401 taataggttc agaaaatact gttcaacaaa aaattcaaga ttttatggaa acttatggtg
    11461 aagtcaacga aattatggca ataagttata tttatgataa agatatgcaa ttagactctt
    11521 atcgtcggtt caagaatgtt ataaatcaga taaatgaaaa aaacacttta taatgtgata
    11581 aataaactaa gtgaaagtat gtatccataa tattaataaa aataaacagt aacagcattt
    11641 tgaataaaag atgtctttat tgttcaatca tttgttttag taatgattca aattcactta
    11701 aaattctaaa gcaaatatga aagcgcccct tcaactttac actgtgttag tgtttatttg
    11761 atggggcgct ttcaaaatat tgaaaagcat atccaaaatt taaagaaatt tatttctctt
    11821 tatcttcatt ttcttttttc tcttcgttat tcgatcctgt atattcattt atcttatctt
    11881 ttacattttt aacttgttca ttatcgctat ttttaaattt ttctactgcg tctttagctt
    11941 tatccataaa actcatatta atcgctcctc ttatatttga ttagtttaat tgaacttatt
    12001 ttttaagttt atcaattgca tcagttattt tgtttttagc attttcaaca acttcttttg
    12061 ctttaccagt cgctttatct tgctgacctt ctttttctaa ttctttgtta tcagtaacgt
    12121 tacctactgt ttctttaaca tttcctttaa attgatcgaa cttactttcg tctgccatag
    12181 tgaaacctcc ttggatgtat atatttatat accactaagg aggttcgcta aacattataa
    12241 tatgaagttt ttatgttata gtatagtatt tatacgatta aatataaaac atgtatccgt
    12301 ctaaatcttc acttgtatct acatattccg ctaaatattt caatgttgta ttatctaaaa
    12361 catctctcac tgcatctctc atgcgaatcc atagttgttt ttgcgcaggt ggttctgatt
    12421 caatactttc aacaaatgta attggacctt ctaacagtct tataatatcc cctgctgaga
    12481 tttcttccgc tggtactctt aattggtatc cacctttagc accgcgtaca cttcgaatta
    12541 accccgcatt tcttaaagga cctacaagct gttctaaata taaatcactc aaattatttt
    12601 cttcagcaat tgactttaat gatatacatc cttgcccctc ttttttagca agagaaatca
    12661 tcaatgtaag tccatatctc cctttagtag aaattttcat tgtataacct cacttaattc
    12721 gaatattgat attcccattt tagcattttt tgagttaaga tagtataaga aaggtgtgac
    12781 aaatgtgagt acagaaccat tagcatcgag aatgcgccca aaaaatatag atgaaatcat
    12841 ttcccaacaa catttagttg gaccaaaagg cattatcaga agaatggttg acacaaaaaa
    12901 attaacttca atgatttttt atggtccacc tggtataggc aaaacaagta ttgccaaagc
    12961 aatttcgggc agtacgcaat ataaattcag acaattgaat gctgtaacta acactaaaaa
    13021 agatatgcaa cttgttgttg aagaagctaa aatgtctggt caagttatct tgttgttaga
    13081 tgaaatacat cgactagata aagctaaaca agacttttta ttacctcatt tagaaaatgg
    13141 caaaatcgtc ttgattggtg ctacaacttc aaatccttat catgctatca atccagcgat
    13201 tcgttcaaga gcgcaaattt tcgagttata tcctttaaat gacgaagatg tgcgccaagc
    13261 gttaactcgt gcaatagaag atgatgagaa tggtttgaaa acatatcaac ccaaaattga
    13321 tgaagatgcc atgacctact tttctacaca aagccaaggt gatgttcgta gtgcgttaaa
    13381 tgcattggaa ttagctgtat taagcgcaga taatgacaaa gacggttatc gacatgttac
    13441 attgcaagat gctaaagact gtttacaaaa aggtgcattt gtaagtgata aggatggtga
    13501 catgcattac gatgttatga gcgctttcca aaaatctatc cgtggtagcg acgtcaatgc
    13561 cgctttacat tatttagcac gattaattga agctggagat ttacctacaa tagttcgacg
    13621 attacttgta attagctatg aggatatagg cttagcctca cctaatgctg gtcagagaac
    13681 acttgctgct attgaatcag cagaacgtct aggtttacca gaagctagaa ttccactaag
    13741 ccaagcagta atcgaactat gcttatcacc taagtcaaat tcagcaatga gtgccattga
    13801 tagtgcattg tccgatatta gaaacggtca tgtgggtcaa attccaaatc atttaaaaga
    13861 tggacattat caaggtgcta aagatctagg ccgatctatt ggttacaaat atccacacca
    13921 atatgttaat ggctatgttt cacagcaata tttacctgat aaacttaaaa acaaaattta
    13981 ttatgaacca aaaacgacat ctaaaagtga acaacaactc aaagaaatat ataacaactt
    14041 acttaaacaa aggccgtaaa aataagatta aatcgattac ctttgatatt ttagctataa
    14101 tcaaatcaaa actgactagt acacgaggca tattctacta tgcttgcgaa ctagtcagtt
    14161 ttagttttat ggtgtggtta cacttgcctt acgttgaaaa ttatgacttt caaaatgaat
    14221 tattgccctt tgtctttaat gcgacgaact ggaatatctt ttaatacgtc attcaccaca
    14281 taactagcac aaattaatcc aacaacactt ggcacaaagg catttgaaga aggtggcatt
    14341 tgtccttttc gattgatagc atttttatct ccaactatat cttttacatc ttctcttatg
    14401 acaattggac tttcatctga aaatacaact ggaatccctt tacgaattcc tagttttttc
    14461 aatttttgac gaataatttt ggccattgga tcggtatgtg ttttagagat atctgaaatt
    14521 gtaaaacgtg ttggatctgt tttatttgca gcacccatac tggaaatcac tttaatccct
    14581 cggtcaagac actctttcat taagtgtact ttgtacatta ttgtatcact tgcatctaca
    14641 aaataatcta tatcgtagtt atcgaaaatt tcttcatatg tctcttctgt ataaaacata
    14701 tgtaagggcg tgactttaca atctggatta attaatttaa tacgttcttc catcaaagaa
    14761 actttacttt gtcctaccgt tgtagttaaa gcgtgtaatt gtctgtttac atttgtaata
    14821 tcaacatcat ctttatctat taatataata tgaccaatat tcgttcttgc taatgcttca
    14881 gcagcaaatg aaccaacacc tccaacgcca agtatgacaa cagtttgttg cttcaataaa
    14941 tctaaacctt gttgtccaat cgctagttca tttcttgaaa attgatgttt cattatttta
    15001 cctctttcac tgatttatac ataagtacat agtaacttaa aattttatat ttagcattat
    15061 cgctttgatt attttcccaa aattcaacga ggaaacattt attaaacgct ataaaaccca
    15121 actaattctt tattaaaaac ttaaagaaac gcataaaaat acgcaagaca aagtcttgcg
    15181 tatcgataga gtccgtattg ccgtagttat aatagcttga tcattcggcc tgttatatac
    15241 aggtgggtgc cctgtttctt gttttgtacg tccttcatat aaggcgtgta cgctgcaaga
    15301 aaacccattg ggctcccttg atcaaagagt gttaggccca aattaaaaag caaacttacg
    15361 aacaactcag atgactatct tatgatgtta tattaccaca taattaaaat taatgaaatt
    15421 ataacaaacc aaagtttatt gattttttaa aatttagtga cgaattcgca aagaaagttc
    15481 ttctaattgt ttatcagaaa cttcaccagg cgcattcgtt aataaacatg tagcagatgc
    15541 tgttttaggg aatgcgattg tatctctcaa gtttgttcta ttagtcaata acatgactaa
    15601 tcggtctaat cctaatgcaa taccgccatg tggtggtgca ccatatttaa atgcatctag
    15661 taagaagccg aactgttctt gtgcttgttc tttagtaaat ccaagaactt cgaacatttt
    15721 ttcttgtaac tcaccatcat gaattctgat tgaaccgcca cctaattcat aaccatttaa
    15781 tactatgtca taagcatttg cctcagcttc ttctggcgca gtgccaagct tagcaatatc
    15841 agcttctttt ggagatgtaa atggatgatg tgctgcaacg taacgtttcg catcttcatc
    15901 atattctaat aatggccaat ctgtcaccca taagaagttt aattttgttt catcgattaa
    15961 acctaattct ttagctaatt tgacacgtaa tgcacctaaa ctttgtgcaa cgacatttgg
    16021 tttgtctgca acaaacatta ctaagtcacc agcttcagca ccagttaatg taagtaatgt
    16081 ttcaacattt tctgtttcaa agaaacgtcc aattggacct gtcaaaccat cttccacaac
    16141 tttaacccac gctaatcctt tagcaccata gatgtttaca aattctgtta aagcatccat
    16201 atctttacga gtatattgtt cagctgcacc tttagcgaca attgctttaa tttcaccatc
    16261 attttcaaca gtatctttaa atactttaaa gtccatatca cgtcctaatt gagaaacgtc
    16321 aattaattcc atttcaaaac gtgtatctgg tttatcagaa ccatagcgac gcatcgcttc
    16381 tttatatgtc atgcgtggga aagcgccatt aatttcaacg cctttaactt ctttaacaac
    16441 ttttttaagc atttcttcac ccatttgcat cacatcttct tgatctacaa aactcatttc
    16501 aatatcgact tgtgtgaatt caggttgacg atctgcacgt aaatcttcgt cacggaagca
    16561 ttttacgatt tggtagtatt tgtcaaatcc actaatcatc aataattgct taaataattg
    16621 tggtgattgt ggtaatgcat aaaattcacc atcatgaaca cgagatggta ctaaatagtc
    16681 acgtgcaccc tcaggtgttg acttcgttag tactggtgtt tcgatgtcaa agaacccttc
    16741 atcatccaaa tattgacgaa tagaacgtgt aatttgatgt ctcattttaa atgtttgcgc
    16801 taactcttga cgacgtaaat ctaaataacg gtattttaat cgaatatttt catcaacgtt
    16861 aacattttct tcatttatag aaaatggtgg tgtctcagat ttattaatca ctttaatatt
    16921 tgtaacttgt acttcaactt ggccagtttt aattttagga ttaactgttt cagggtcacg
    16981 cttcgtaact gtaccttgaa cttctacaac atattcagaa cgtactgttt cagcaatttt
    17041 caatgcctct tctgaaaatg caggattaaa cacgacttgt acaattcctt ctctatctct
    17101 taaatcaacg aaaatcaatc cacctaggtc acgacgattg ttaacccatc cttttaatgt
    17161 aatttcttgt cctaaaaatg cttcagtaac taatccacaa taagttgttc tcttactcat
    17221 attttaacgc cctctctact tcttaaaata ttcgactaat gcgtctaatt caattgtttc
    17281 agattcacca gttgtcatat ttttaacatc gattttatta ttttctaatt cttgatcacc
    17341 aataacgatt gtaaacttgg cacctaaacg gtctgcttgt ttcatttgtc ctttaatttt
    17401 acgctgtaag tagtctttat ctgctttaat accattatgt ctcaaatgat ttaataactt
    17461 cacagcatat cgatctgctt gatcacccat tgtaacaatg aataaatcta agttttcttc
    17521 aatatctaat tcgatacctt cttcttcaag tgcaagcaat aatcgttcta tacttagcgc
    17581 aaaaccaata cctgtttcac ttggaccatc tagcaattct aataaaccat tataacggcc
    17641 accaccacaa agcgttgtaa tggcaccatc atagttagga ttatccatca ttaattcaaa
    17701 tgctgtatgt gtataataat ccaatccacg aactaagtta ggatcttcaa tatatggaat
    17761 acctaaatca tctaaataag cttttacttg ttcataatat gccttagatt cctcatttaa
    17821 gaaatcagtg attctaggtg cagtcttaat cgcttcttta tcacggtcaa ctttacaatc
    17881 caaaattcgc atcggatttg tatgcaaacg tgattgacaa tctgaacaaa attcatgaat
    17941 tactggttca aagtgtttca ctaacgcttc gttatattct tttcgagacg ccatatcccc
    18001 tacactatta ataacaagct ttaaatgttt taatccaaat gattgataaa tatgcataac
    18061 catagctaat acttctgcat ctacgctagg attttcagca ccaatagctt ctacaccaaa
    18121 ttgattaaat tgacgatagc gccctttttg cttacgttca tatctaaaca tcggtccatt
    18181 gtaataaagt ttaattggtt ggtttggatt accttgcatt ttatgttcaa tatatgaacg
    18241 cacaactgca gctgttccct caggtcttaa tgtaatactt ctatcgcctt tatctttaaa
    18301 tgtatacatt tctttttgta cgacatcggt tgaatcacca acacctcttg caaaaagatc
    18361 tgtactttca aaaatt
//
LOCUS       DANBWH010000032        18357 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.032, whole genome shotgun sequence.
ACCESSION   DANBWH010000032 DANBWH010000000
VERSION     DANBWH010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18357
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.032"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(72..512)
                     /locus_tag="PPY92_000757"
     CDS             complement(72..512)
                     /locus_tag="PPY92_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HDJ2624778.1"
                     /translation="MASIGFKTYNFNHISYSKNKELDIENVDTFEVNSDFSAKVAFEN
                     EEAYIIMDCQIGNNKPNCPFIINMQLTGVFDIEYENDVEDKNSLKILLTQNAIAILYP
                     YIRSLVSDLTSKANMFDTFIMPVVNVAKMMEENNRIEIVGLDKK"
     gene            complement(512..808)
                     /locus_tag="PPY92_000758"
     CDS             complement(512..808)
                     /locus_tag="PPY92_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusB"
                     /protein_id="HDJ2624779.1"
                     /translation="MGLKLKEYINDSNRKLDKETVRKHLVDILNYTVEENSFLDQMTV
                     GKRLYSNQYKNSPSVSLTVNHKIKINEVTSDFSYKFSDNETQTFNLSNELGVVA"
     gene            complement(829..1401)
                     /locus_tag="PPY92_000759"
     CDS             complement(829..1401)
                     /locus_tag="PPY92_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624780.1"
                     /translation="MCTTIDIRGYHGTDRSCYDNLEINKEFPVNKLPSDLGNGLYLFI
                     DRKNKVGEAIKNAKNYLDRWKPKYEKKIIAQIDIKVKENRLMDLDDHDNQYEFNSFVE
                     ENEETIYNELDKLINNNTKSRGNIDGLIIEIMIREYNLEIDAIQKETYTQFDKSKKRK
                     RSNIPNGKELCIRQNDIIKNKCIYNENVVT"
     gene            complement(1616..2185)
                     /locus_tag="PPY92_000760"
     CDS             complement(1616..2185)
                     /locus_tag="PPY92_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDJ2624781.1"
                     /translation="MSELTAKQARFVNEYIRTLNVTQSAIKAGYSANSAHVTGCRLLK
                     KPHIKQYIQEQKDKIIDENVLTAKELLHVLTNAAVGDETETKEVVVKRGEYKENPQSG
                     KVQLVYNEHVELIEVPIKPSDRLKARDMLGKYHKLFTDKHDINGNVPIFINIGEWDGD
                     DEDLDKTVQEVSNANPNHTVIVDDIPLED"
     gene            complement(2182..2394)
                     /locus_tag="PPY92_000761"
     CDS             complement(2182..2394)
                     /locus_tag="PPY92_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island family protein"
                     /protein_id="HDJ2624782.1"
                     /translation="MTDDNVDDHIIKNHLEMIVDRVANDKEFYIFDSLIQGRSFKDIS
                     NVLECSEQSVRLWYETLLDKIVEVIE"
     gene            complement(2526..3053)
                     /locus_tag="PPY92_000762"
     CDS             complement(2526..3053)
                     /locus_tag="PPY92_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HDJ2624783.1"
                     /translation="MKLLKTKNCLYYRNGDNKLSEYQLLTQFNPAFINKKIKMCEFQI
                     ESMYHMSASTTTCDEIMGVVSVSYPIEKLVIKIIETKAGLQNYKNRSISNMVLLKTVL
                     NHYTEKEQKQVVKYMRSNGRYKPYSVIERLQVDLYQASIKQRSERQKQRNTAIENSKI
                     ARVNAYHQSSHVKVV"
     gene            complement(3106..3759)
                     /locus_tag="PPY92_000763"
     CDS             complement(3106..3759)
                     /locus_tag="PPY92_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624784.1"
                     /translation="MTTTTITGFTWDVYFNDRRYRNLLGDFEDLITETKSLIRQGYKM
                     DVIKNKMDNKALSLQYKFKELGQILLDEHEEKIVEIQQKEKESSYENPQVEMLKRQDI
                     EAKVNLIDAEELFNLVYNANPKTTNVYELNIYKKAIESRLTEDENVRLKPYFDVLVEK
                     VIYPYRNNEEYQKLEYNYNVLRQFGLQNNGQPVIKDNDGDIEIINIQSKYNEVFRNA"
     gene            complement(3790..4131)
                     /locus_tag="PPY92_000764"
     CDS             complement(3790..4131)
                     /locus_tag="PPY92_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001668138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HDJ2624785.1"
                     /translation="MQSIAEKETYHLPTEHLQVFNVIKNTSNKYITKTKILNQLGYEY
                     NSSNERWLRRVINSLVYDYGYPIGCSYKPSERGYYIITTEQEKQQAMRSIKKLADGSM
                     KRYEALKRIEV"
     gene            complement(4667..5308)
                     /locus_tag="PPY92_000765"
     CDS             complement(4667..5308)
                     /locus_tag="PPY92_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012990921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HDJ2624786.1"
                     /translation="MNIETVVNEFETRAGTLLRYYTGLLESSRETHFAFKIYNDPFDM
                     VYVMMKGKLYGHVYIKDCKVRQSFELASPKHTEGLIRSIEGHYVGYELHDGKQLSISD
                     MMASHLFEDEYFMYGLQTYAESNNSDVFEYLENGFDTDTLEGIQSSNTDVIANIEMLY
                     QIATGINEPAPELVEGLKLVTEFVQDEKATQEDYKALERKLTELKSSYYSLNK"
     gene            complement(5305..5589)
                     /locus_tag="PPY92_000766"
     CDS             complement(5305..5589)
                     /locus_tag="PPY92_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HDJ2624787.1"
                     /translation="MNDKEKIYNQLHHDAPIQIIPAPENLFVEYIEADEVWYSPVVCM
                     ALSKAHNINFYDSDDVGCIDKAATCSIKKFNPETGEFEQFSKMAQKEITQ"
     gene            complement(5591..5953)
                     /locus_tag="PPY92_000767"
     CDS             complement(5591..5953)
                     /locus_tag="PPY92_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012990923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HDJ2624788.1"
                     /translation="MNKNQLKSEILEYIKAHAGTSFVEIERVFEENNFDYKGDGAYTS
                     GQHPNVVFWIGWNQEAFDVIAELKKDRRIEMDICEPIVYMVDGKGLDLPIVSSKNIKT
                     DHWLPVTFTISKKETECV"
     gene            complement(6239..7708)
                     /locus_tag="PPY92_000768"
     CDS             complement(6239..7708)
                     /locus_tag="PPY92_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated protein E"
                     /protein_id="HDJ2624789.1"
                     /translation="MENVTNDEVFEMIDSRTGVLNANDWKSQLRRSATTQALKKTTTN
                     AEIILCNDESLKGLVQYDAFEKVTKLKRLPYWRSKGDANYYWADIDTTHVISHIDKLY
                     NVQFSRDLIDTVIEKEAYQNRFHPIKSMIESKSWDGIKRIETLFIDYLGAEDNHYNRE
                     VTKKWMMGAVARIYQPGIKYDSMIILYGGQGVGKSTAVSKLGGHWYNQSIKTFKGDEV
                     YKKLQGSWICEIEELSAFQKSTIEDIKGFISAIVDIYRASYGKRTERHPRQCVFVGTT
                     NNYEFLKDQTGNRRFFPITTDKNKVTKSPFDDLTPVVVQQMFAEAKVYFDENPTDKAL
                     LLDKEASEMALKVQEAHSEKDALVGEIEEFLERPIPSDYWYRTLEEKRVSAHDVIDQD
                     YIKLYGDGKLIELPNAKPGAYVWRDKVCSMEIWKVMMKRDDQPQQHHLRKIDKALRNT
                     SYCGQSKSRHRFGEGIGRQYGFGINLISYYQGLKSKEQK"
     gene            complement(7725..8594)
                     /locus_tag="PPY92_000769"
     CDS             complement(7725..8594)
                     /locus_tag="PPY92_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HDJ2624790.1"
                     /translation="MNEIKLEYDTHVSVVHYESLDSRSFKSFSKPKWSKLINKLSVPI
                     EANYKYARGVAVYGDIKNGANDHGEIIKKHRNDVNVVYRDVIVLDYDEINDLKQLHGA
                     ISSALSNVAWFWHTSYSHRTEQARIRLYIPLNERISADDYRKYSKVLANKIGHKVDEG
                     SYQPSRCFALPVIQKGHIFIKRVNDCPIIDVDMLEQWSKELEQSNASPNVIGYTRRDS
                     AYWRDIAFGVSEGERNSTLASITGYLLRRYVDPNLVYGLVSAWASVCKPPINQSEVNN
                     TFKSILKKDSKSS"
     gene            complement(8661..8978)
                     /locus_tag="PPY92_000770"
     CDS             complement(8661..8978)
                     /locus_tag="PPY92_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1474 family protein"
                     /protein_id="HDJ2624791.1"
                     /translation="MNWEIKDLMCDIEVIKEKINDVAIKHGWFVEDKFVKNELETKQE
                     HINFSASYLEHRIQNEHTVELLQVYLKEFGELIQKFHEIEKASSENFGEESDDAKKLK
                     ITE"
     gene            complement(8981..9190)
                     /locus_tag="PPY92_000771"
     CDS             complement(8981..9190)
                     /locus_tag="PPY92_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HDJ2624792.1"
                     /translation="MNNEQKEVIEHVVYQLELSIMNNFESYEHTEYVDGIEVVSEISP
                     EKHLELIMKWCAQELKNNFQLEKGE"
     gene            complement(9183..9329)
                     /locus_tag="PPY92_000772"
     CDS             complement(9183..9329)
                     /locus_tag="PPY92_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HDJ2624793.1"
                     /translation="MKIYLTYICLVSLLTILLLAISNMYVAFSVYAWLITLGCNLTGG
                     LENE"
     gene            complement(9326..9643)
                     /locus_tag="PPY92_000773"
     CDS             complement(9326..9643)
                     /locus_tag="PPY92_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDJ2624794.1"
                     /translation="MFNINIDENEAREILEQAINARVEELAKEKYFMTYKELSNYLNL
                     SKPTIEELLINNGMKYYMVGSTYRFKKSDVDAFMEQLTAHMNIQNNDFKQVNIKKLLE
                     ASQ"
     gene            complement(9647..9853)
                     /locus_tag="PPY92_000774"
     CDS             complement(9647..9853)
                     /locus_tag="PPY92_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDJ2624795.1"
                     /translation="MKNNLSMLMGKERINASKLSKETGISRTTIYGLYHEKTESPDTK
                     TVLTLCDYFGITLNEFFGIEKKEV"
     gene            10006..10728
                     /locus_tag="PPY92_000775"
     CDS             10006..10728
                     /locus_tag="PPY92_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDJ2624796.1"
                     /translation="MKFSEMLKKYRTKENLSINKLAKLSGVSTTYISKLEKNDRSYPT
                     VEIIFNLAYGIIMKIKEKYDGIENSDDFLYPQIEEIISSFATSEDSNLDEENKNTIID
                     DFIMFMERKEKEFLNKSFGDNKEIYENKIALVSNSMNYKKTDYPYFDLKWLLSQNNFE
                     VFYGRDFITNFATIEDSKLNTKSMYFYNILDKEDLKTIQRLIEVYLESKYPKIKDKDD
                     FFVLATDKQNRIKNTIDWYNIN"
     gene            10742..11914
                     /locus_tag="PPY92_000776"
                     /pseudo
     CDS             10742..11914
                     /locus_tag="PPY92_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002476300.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(11983..13599)
                     /gene="groL"
                     /locus_tag="PPY92_000777"
     CDS             complement(11983..13599)
                     /gene="groL"
                     /locus_tag="PPY92_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500736.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDJ2624797.1"
                     /translation="MVKQLKFSEDARQAMLRGVDQLANAVKVTIGPKGRNVVLDKEFT
                     APLITNDGVTIAKEIELEDPYENMGAKLVQEVANKTNEIAGDGTTTATVLAQAMIQEG
                     LKNVTSGANPVGLRQGIDKAVKVAVEALHENSQKVENKNEIAQVGAISAADEEIGRYI
                     SEAMEKVGNDGVITIEESNGLNTELEVVEGMQFDRGYQSPYMVTDSDKMVAELERPYI
                     LVTDKKISSFQDILPLLEQVVQSNRPILIVADEVEGDALTNIVLNRMRGTFTAVAVKA
                     PGFGDRRKAMLEDLAILTGAQVITDDLGLDLKDASIDMLGTASKVEVTKDNTTVVDGD
                     GDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKL
                     RIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIEAEGDIETGVNIVLKALTAPVRQ
                     IAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNMLEAGIVDPTKVTRSALQHAASVA
                     AMFLTTEAVVASIPEKNNDQPNMGGMPGMM"
     gene            complement(13675..13959)
                     /gene="groES"
                     /locus_tag="PPY92_000778"
     CDS             complement(13675..13959)
                     /gene="groES"
                     /locus_tag="PPY92_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDJ2624798.1"
                     /translation="MLKPIGNRVIIEKKEQEQTTKSGIVLTDSAKEKSNEGVIVAVGT
                     GRLLNDGTRVTPEVKEGDRVVFQQYAGTEVKRDNETYLVLNEEDILAVIE"
     gene            14134..14877
                     /locus_tag="PPY92_000779"
     CDS             14134..14877
                     /locus_tag="PPY92_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDJ2624799.1"
                     /translation="MTRLWASLLTVIIYILSQFLPLLIVKKLPFVQYSGIELTKAVIY
                     IQLVLFLIAATTIILINLKIKNPTKLELEVKEPKKYIIPWALLGFALVMIYQMVVSIV
                     LTQIYGGQQVSPNTEKLIIIARKIPIFIFFVSIIGPLLEEYVFRKVIFGELFNAIKGN
                     RIVAFIIATTVSSLIFALAHNDFKFIPVYFGMGVIFSLAYVWTKRLAVPIIIHMLQNG
                     FVVIFQLLNPEALKKATEQANFIYHIFIP"
     gene            complement(14902..16071)
                     /locus_tag="PPY92_000780"
     CDS             complement(14902..16071)
                     /locus_tag="PPY92_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624800.1"
                     /translation="MSYHWFKKMLLSTSILILSSSSLGLATHTVEAKDNLNGEKPTTN
                     LNHNITSPSVNSEMNNNETGTPHESNQTGNEGTGSNSRDANPDSNNVKPDSNNQNPST
                     DSKPDPNNQNPSPNPKPDPDNPKPKPDPDKPKPNPDPKPDPDKPKPNPNPKPDPNKPN
                     PNPSPDPDQPGDSNHSGGSKNGGTWNPNASDGSNQGQWQPNGNQGNSQNPTGNDFVSQ
                     RFLALANGAYKYNPYILNQINKLGKDYGEVTDEDIYNIIRKQNFSGNAYLNGLQQQSN
                     YFRFQYFNPLKSERYYRNLDEQVLALITGEIGSMPDLKKPEDKPDSKQRSFEPHEKDD
                     FTVVKKQEDNKKSASTAYSKSWLAIVCSMMVVFSIMLFLFVKRNKKKNKNESQRR"
     gene            16268..16894
                     /locus_tag="PPY92_000781"
     CDS             16268..16894
                     /locus_tag="PPY92_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDJ2624801.1"
                     /translation="MGLFKKDKKAMTFDNAMEERRSIYNLKDSISISDDELESMIAHA
                     VKHVPSSFNSQSTRIVLLLNDNNNKFWDNTKAILKEVMGENRDFEPTEQKIDNFKHSY
                     GTILFYEDQDVVSGLQEQMPNYYDNFAIWSTQTNAMHQFAIWTALATKGIGASLQHYN
                     PLVDEMTSKEFNIPKSWKLIAQMPFGDIREAAGEKTFNPVEDRFVIKK"
     gene            17255..18040
                     /locus_tag="PPY92_000782"
     CDS             17255..18040
                     /locus_tag="PPY92_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HDJ2624802.1"
                     /translation="MKVQIYQLPIIFGDSSKNETQITQWFEKNMNAEVDVVVLPEMWN
                     NGYDLEHLNEKADNNLGQSFSFIKHLAEKYKVDIVAGSVSNIRNYQIFNTAFSVNKSG
                     QLINEYDKVHLVPMLREHEFLTAGENVAEPFQLSDGTYVTQLICYDLRFPELLRYPAR
                     SGAKIAFYVAQWPMSRLQHWHSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINP
                     NGDLVGELNESADILTVDLNLNEVEQQRENIPVFKSIKLDLYK"
BASE COUNT         5766 a         3369 c         2484 g         6738 t
ORIGIN      
        1 gtggcgtttt taagttagct tagtattcat taatcattta gttttcataa ttattatttt
       61 tctaaaaatc actatttttt atccaagcca acaatttcaa ttctattatt ttcctccatc
      121 atcttagcaa cattaacaac aggcattata aaagtatcaa acatatttgc tttcgatgtg
      181 agatcagata ctaaactacg aatataagga tataaaattg caatagcatt ttgtgtgagt
      241 aatattttta aactattttt atcttcaaca tcgttttcat attcgatatc aaagactcct
      301 gttaattgca tatttataat aaacggacaa ttgggtttat tattacctat ttggcagtcc
      361 attataatgt aagcttcttc attttcaaag gctacttttg cagaaaaatc actattcact
      421 tcaaaagtat caacattttc aatatctaat tctttatttt tactataact aatatgatta
      481 aagttgtaag ttttgaaacc tatactagcc attacgctac tactcctaac tcatttgaaa
      541 gattaaaagt ttgagtttca ttatcagaaa atttatagct aaaatcactc gtcacttcat
      601 ttattttaat tttatgattt acagttaatg atacagaggg cgagttttta tattgatttg
      661 aatatagtct tttaccaact gtcatttgat ctagaaaact attttcttca acagtgtaat
      721 ttagtatatc tactaagtgc tttctaactg tttctttatc caatttccta ttactatcat
      781 ttatatactc ttttaatttc aaacccattt atatcacctc tacttatatt aagttaccac
      841 attttcatta taaatacact tattcttaat aatatcattc tgtcttatac aaagttcttt
      901 accgttaggt atgttagatc tttttctttt tttgctttta tcaaattgag tataagtttc
      961 tttttggata gcatcaattt caagattata ctctctaatc ataatctcaa ttattaagcc
     1021 gtctatatta cctcgacttt ttgtattatt attaatcaac ttatctaatt cattgtatat
     1081 tgtttcttca ttttcttcaa cgaatgaatt aaattcatat tgattatcat gatcatctaa
     1141 atccatcaat ctattctctt ttacttttat atctatttgt gcaatgattt ttttctcata
     1201 ttttggtttc catctatcta aataattttt agcattttta attgcttctc caaccttatt
     1261 ttttctgtct ataaatagat aaagaccatt acctaaatca gatggtaatt tattaacagg
     1321 gaattcctta tttatttcta aattatcata gcaacttcta tctgtaccat gataacccct
     1381 tatatctatt gttgtgcaca tagtaatact ccttatgaaa aacaattggg tttaattata
     1441 caactataca agcagattat aaagcctttt acttttaatc actatcataa agtctaataa
     1501 acgctaaatc gcataggctt ggcgtctatt tacattactc ctcaaaccca cctatatcat
     1561 caacaatttc tggtgtcaca agactcacat ctagtttatc tgtaaatatt cctcattaat
     1621 cctctaacgg aatatcatcc acaatcacag tatgattagg attagcgtta gatacctctt
     1681 gtaccgtctt atctaaatct tcatcatcgc catcccattc accaatatta atgaatatag
     1741 gcacattccc gttaatatca tgcttatctg taaataactt atggtatttc cccaacatat
     1801 cacgagcttt taaacgatca ctaggtttta ttggtacctc tatcagttca acatgttcat
     1861 tatagactaa ttgtactttg ccactttgtg gattctcttt atattctcca cgctttacta
     1921 caacttcttt cgtttctgtt tcatcaccga ctgccgcatt cgtaagcaca tgtagtaact
     1981 cttttgcggt taatacattc tcatctataa tcttatcttt ttgttcttgt atatattgct
     2041 tgatgtgtgg cttcttcaat aacctacacc ctgtcacatg tgcgctattt gcgctatagc
     2101 ctgcttttat ggcactttgt gttacattaa gtgttcttat atactcattc acaaaacgcg
     2161 cttgttttgc cgttaactca ctcattctat cacctccaca attttatcta ataaggtttc
     2221 ataccataat cttacagatt gttctgaaca ctctaagaca ttgctaatat ctttaaaact
     2281 acgtccttgt attaaagaat cgaaaatata aaactcttta tcattagcta ctcggtcaac
     2341 aatcatttct aagtgattct ttataatatg atcatcaaca ttatcgtctg tcatccattc
     2401 attagaattt tcatcaccta ttgaaaagaa ttcatcagta tttatatcat catctattaa
     2461 tacatcactt ctagttcgct tatgataatc acacacgaag ccttttattt gctttttatc
     2521 cattgttaca ccacttttac atgtgaagat tgatgataag catttactcg tgcaatcttg
     2581 ctattttcaa ttgctgtatt tctttgtttt tgacgttctg aacgttgttt aatacttgct
     2641 tgatacaaat caacctgtaa gcgttcaatg acgctgtatg gcttatatcg tccatttgaa
     2701 cgcatatatt ttacaacttg cttctgctct ttttctgtat aatgatttag taccgttttc
     2761 aacaacacca tattacttat agatcgattt ttatagtttt gtaatcctgc ttttgtttca
     2821 ataattttga taactagttt ttcaattgga tatgagacag agacgacccc cattatttca
     2881 tcacatgttg tggtcgacgc actcatatgg tacatacttt caatttggaa ttcacacatc
     2941 ttaatttttt tattaataaa tgctgggtta aattgcgtta atagttgata ctcagatagt
     3001 ttattgtcgc cattacgata atataaacaa ttcttcgttt taagcagttt catacgttca
     3061 ctcctataaa gagagcctac ccaaattgga taggctattt ttgatttaag cgttacggaa
     3121 cacttcgtta tacttacttt gaatgttaat aatttctata tcgccatcat tatctttgat
     3181 aactggttgc ccgttatttt gtaatccaaa ctgtcttaaa acattatagt tatactctaa
     3241 tttttgatat tcttcattat ttcgatatgg ataaattacc ttttctacca atacatcaaa
     3301 gtaaggtttt aaccttacat tttcatcttc agtaagacga ctttctatcg cttttttata
     3361 gatattaagt tcatatacat tagtggtttt aggattggca ttataaacaa gattaaatag
     3421 ttcttctgca tcaattaaat ttactttcgc ctctatgtct tgacgtttca acatttcaac
     3481 ttgtggattc tcatatgaag attctttctc tttttgttgg atttctacta ttttttcttc
     3541 atgttcatct aataatattt gtcctaattc tttgaattta tattgtaggc tcaaagcctt
     3601 attatccatt ttatttttaa taacatccat tttatagcct tgtctaatta atgatttcgt
     3661 ttctgttatt agatcttcaa aatctcctaa caaatttcta taacgtctat cattaaaata
     3721 tacatcccac gtaaaacccg tgattgttgt agttgtcatt tataagtacc tctttcttta
     3781 gtttttgttt tacacttcaa ttcgtttcaa agcttcatag cgtttcatac tgccatcagc
     3841 taatttctta atacttctca tcgcttgttg cttttcttgt tctgtcgtaa tgatgtaata
     3901 accacgttca ctaggtttat aactgcatcc gataggatag ccataatcat atactaatga
     3961 attgattact cttcgtaacc atcgttcatt gcttgaatta tattcatatc ccaattgatt
     4021 taagatttta gttttagtaa tatacttatt ggacgtattt tttatcacat tgaaaacttg
     4081 caggtgttcg gtgggtagat gatacgtctc tttttctgcg atactttgca tttctacacc
     4141 tctttctttt aattatttca tacctaaatt ataccatttt tacaggtcta aaacaaactt
     4201 acgttcgctt tatagcgcgt tttatcaatt gtttagctta tcatatataa cacttataaa
     4261 atcatgttat aaacttaacg ttaggctttt cacattaacc taatatagaa cttaagttcg
     4321 gtaaaataac acgaacaaac agcgaacaaa cttaactttt aggcctatgc caaaaacaca
     4381 aactttagct tgtattagcg ttaacaaagt tctcacacct tgcacaaatc ttgccatttt
     4441 ttcaattctc aaagactgta taccttccga ttttaaaagc taacaccttt atataacctt
     4501 attattttca aagccataaa acagctcaat atcaacattt cacacttttt ttaatttcgc
     4561 acacctatct attaactcac tattgttgca acctttacat tttaaaactt ctatacctta
     4621 tacttttaca ttaggagcca cacaccacgt gtgactccat atttaattac ttattcaaac
     4681 tgtaataaga tgacttcaac tcagttaact tacgttctaa agccttgtaa tcctcttgtg
     4741 tagccttctc atcttgtaca aactcagtta ctaattttaa tccctcaact aactctggtg
     4801 ctggttcatt gattcccgta gctatctgat acaacatttc aatattcgct atcacatcag
     4861 tattactcga ttgaatgccc tcaagtgtat cagtatcaaa tccattttct aggtactcaa
     4921 acacatcact attatttgat tctgcatatg tttgtaatcc atacataaaa tattcatctt
     4981 caaacaaatg actagccatc atatcactaa tagaaagctg tttaccgtca tgtaattcat
     5041 aacctacata atgcccctct atgcttctta taagcccctc agtgtgctta ggtgacgcta
     5101 attcaaacga ttgccttact ttacaatctt taatatatac atgtccgtat aatttacctt
     5161 tcatcatcac gtataccata tcaaatggat cattatatat tttgaaagcg aaatgcgttt
     5221 ctctactact ttctaataaa cctgtgtaat accttaataa cgtgcctgct cgtgtttcaa
     5281 attcatttac tacagtttct atgttcattg cgttatctcc ttttgagcca ttttgctgaa
     5341 ttgttcaaac tcacctgtct caggattaaa ttttttaatg ctacatgtgg ctgctttatc
     5401 aatgcacccc acatcatcac tgtcataaaa attaatatta tgcgctttac ttaaagccat
     5461 acatacaact ggtgaatacc atacttcatc agcttctata tattcgacaa ataaattttc
     5521 gggtgctggt ataatttgaa ttggtgcatc atgatgaagt tgattataaa ttttctcttt
     5581 gtcattcata ttagacacac tccgtttctt tcttactaat agtaaacgtg acaggtagcc
     5641 aatgatctgt tttaatgttt ttcgaactta caataggcaa atccaaacct ttaccatcaa
     5701 ccatataaac aattggctca caaatatcca tctcaatacg tctgtctttt ttaagttcag
     5761 cgataacatc aaacgcttct tgattccacc caatccaaaa cacaacattg ggatgttgac
     5821 cacttgtata tgcgccgtca cctttataat caaagttatt ttcttcaaat acacgttcta
     5881 tttctacaaa tgatgtacca gcatgcgcct ttatatattc taaaatttct gactttaatt
     5941 gatttttatt cattttcttc ctcctaattt ttgataggtg tcccatttcg ctattttcaa
     6001 tatgtttcag gacattgtca ggacaatgct caaacaccac tctcccaatg gattaaagtt
     6061 atctgtccca ttgtcccact gttttatgcc tacacttata tattttttaa tgtgtatata
     6121 ttattttaaa agtttagggt aaaagttgcg ggacacaggg acaaacataa cataatgctt
     6181 actgccacaa gagattgaag gtgtcctacc attgtccgat aatcgtcccg ttgtcccgtt
     6241 atttttgttc tttgcttttt aaaccttgat aataggatat taaattaata ccaaaaccat
     6301 attgtctacc aataccttca ccaaatctat gacgcgactt actttgccca caataacttg
     6361 tatttcttaa cgctttatca atttttctta aatggtgttg ttgtggttgg tcatctcgtt
     6421 tcatcatcac tttccaaatt tccatgctac ataccttgtc acgccataca taagcacctg
     6481 gttttgcatt cggtaattca atcaatttac catcaccata taatttaata tagtcttggt
     6541 ctataacatc atgcgcagac actctttttt cttctaacgt tctataccaa tagtctgacg
     6601 gaataggacg ttcaagaaat tcttctattt ctccaactaa agcatctttt tcagaatgag
     6661 cttcttggac ttttaaagcc atttcactcg cttctttatc tagcaacaat gctttatctg
     6721 tcggattctc atcaaaatat actttagctt cggcaaacat ttgttgcaca acaactggtg
     6781 ttagatcgtc aaatgggctt ttagttactt tatttttatc tgtcgtaata gggaaaaaac
     6841 gacgattgcc tgtttggtct tttaaaaact catagttatt ggttgtccct acaaacacac
     6901 actgtctagg atgacgctct gtgcgtttac catacgaagc tctataaata tctacaatgg
     6961 cacttataaa acccttaata tcttcaatag tagacttttg aaatgccgac agttcttcaa
     7021 tttcacatat ccaagaaccc tgcaatttct tatagacctc atcaccttta aacgttttaa
     7081 tactttggtt ataccaatga cctcccaatt tactcactgc cgtagatttc ccaacacctt
     7141 gaccaccata taaaataatc atggaatcat atttaatacc tggctgatag attctagcaa
     7201 ctgcacccat catccatttc tttgtaactt ctcgattgta gtgattatct tcagcaccta
     7261 aataatcaat gaagagcgtt tcaattcttt tgattccatc ccatgattta gattcaatca
     7321 tcgatttaat agggtggaat ctattttgat atgcttcctt ttcaattaca gtatcaataa
     7381 gatcgcggct aaactgcaca ttatacaatt tatcaatatg tgaaatcaca tgtgtggtat
     7441 ctatatcagc ccaataataa ttcgcatccc cttttgacct ccaatacggt agacgtttca
     7501 gcttggttac tttttcaaaa gcgtcatatt gtactagccc ttttaaactc tcatcattac
     7561 acaatatgat ttcagcattt gtagtcgttt ttttcaatgc ttgtgtagtg gcagaacgcc
     7621 ttaattgact tttccaatca ttagcattta aaacaccggt tctactatca atcatttcaa
     7681 acacttcatc atttgttaca ttttccaaac aaaaacctcc atttctaact gcttttacta
     7741 tcttttttca aaatactttt aaaagtattg tttacttcac tttgattaat aggtggtttg
     7801 catacacttg cccacgcact cactaaccca taaactaagt ttggatctac atacctacgc
     7861 aaaagataac ctgtaattga agccaatgtt gaattgcgct ctccctcact tacaccaaaa
     7921 gctatatctc gccaatacgc actatcacgt cgcgtgtacc ctataacatt aggactagca
     7981 tttgattgtt caagttcctt cgaccactgt tcgagcatat caacatcgat aattggacag
     8041 tcattcactc gcttaataaa tatgtgccct ttttgaataa ctggtaatgc aaaacatcta
     8101 cttggctgat atgaaccttc atcaacttta tgaccaattt tgtttgctaa tacctttgaa
     8161 tatttacgat aatcatctgc acttattcgc tcatttagag ggatatacag gcgtattcta
     8221 gcttgttcag ttctgtgcga gtaacttgtg tgccaaaacc atgcaacatt gcttaaagct
     8281 gagctgattg ctccatgtaa ttgctttaaa tcatttattt catcgtaatc aagtacaatc
     8341 acatctctgt atacgacatt aacgtcattg cgatgctttt tgataatttc accatgatca
     8401 tttgcaccgt ttttaatatc accgtaaaca gcaacaccac gtgcatactt ataatttgct
     8461 tctataggca cagacagttt attaattaac ttactccatt taggttttga aaagctctta
     8521 aatgaacgtg agtctaaact ttcataatgt accactgaaa catgtgtgtc atattctaat
     8581 ttaatttcat tcattttttg cacctcttaa tgaaacaaca gagcaaagat gttataataa
     8641 aaatagttat tttttattaa ttactctgta atttttaatt tctttgcgtc atctgattcc
     8701 tcgccaaagt tctcagatga tgctttttct atttcatgaa acttttgtat aagttcaccg
     8761 aattctttta agtacacctg taataactca actgtatgtt cattttgtat acgatgttct
     8821 aaatagctag cagaaaaatt aatatgttcc tgttttgttt ctaattcatt tttgacaaat
     8881 ttatcttcaa caaaccaacc atgtttgata gctacatcat tgattttttc ttttatcact
     8941 tcaatatcac acatcaaatc tttaatttcc caattcatat ttattctcct ttttctaatt
     9001 gaaaattatt ctttaattct tgtgcgcacc atttcattat caattctaag tgcttttcag
     9061 gactgatctc tgaaaccact tcaataccat caacatattc cgtgtgttca taactttcaa
     9121 aattattcat gatacttaac tcaagttgat aaaccacgtg ttctattact tctttttgtt
     9181 cattattcat tttctaatcc tcctgttaaa ttacatccta aagttattag ccatgcataa
     9241 acgctaaaag caacatacat gttagatatt gctagtaata gtattgttaa caatgaaact
     9301 aagcagatat aagttaagta tattttcatt gacttgcctc caataacttt ttgatattga
     9361 cttgtttaaa gtcgttattc tggatattca tatgagcagt aagctgttcc atgaatgcat
     9421 ctacatctga ctttttgaat ctgtatgtag atccaaccat gtaatacttc ataccattat
     9481 taattagtaa ttcttcaatc gttggtttac ttaaattcag atagttagac aattctttgt
     9541 aagtcatgaa atatttctct ttcgctaatt cttccacacg tgcatttata gcttgttcga
     9601 gtatctcacg agcttcgttt tcatcaatat taatattgaa catttgttat acctcctttt
     9661 tttcaatacc aaaaaactca ttaagagtta ttccaaagta gtcacataac gttagtactg
     9721 ttttcgtgtc tggactttca gttttttcat gatataaacc ataaattgtt gttcttgaaa
     9781 ttccagtttc tttgcttaat ttcgacgcat taattctttc tttccccatc aacatgctta
     9841 aattattttt caattaagtc acctcctctt tgtttgtatg tacaattata tattttgtgt
     9901 acaaaacaat catacaactt ttcaggttgc actgtcaaca aatacttttt ttgattgtat
     9961 gtacaaccaa aaaaagagta tactactatt aaatgaggtg ataatttgaa attcagcgaa
    10021 atgttaaaaa aatatagaac aaaagaaaac ttgtcaatta ataaattagc taaattgtca
    10081 ggtgtatcta ctacttacat tagtaagctc gaaaaaaatg atcgttcata tccaactgtt
    10141 gagataatat tcaacttagc ttatggaata attatgaaga taaaggaaaa atatgatggt
    10201 attgaaaatt ctgatgattt tttatatccc caaatagaag aaataatatc ttctttcgct
    10261 acaagtgaag atagtaattt agatgaagaa aacaaaaaca caataataga tgactttatt
    10321 atgtttatgg aacgaaaaga aaaagaattt ttgaataaaa gttttggtga taacaaagaa
    10381 atttacgaaa acaaaatagc tttggtttcc aactcaatga attataaaaa aacagattat
    10441 ccttattttg atttaaaatg gttactttct caaaataatt tcgaagtatt ttatggaaga
    10501 gattttataa ctaattttgc aacaattgaa gatagtaaat taaacactaa atcaatgtac
    10561 ttttacaaca ttttagataa agaagattta aaaaccatcc aaagactaat agaagtttat
    10621 ctagaaagca aatatcctaa aataaaggat aaagatgact tttttgtttt ggctactgat
    10681 aaacaaaata gaataaaaaa caccatagat tggtataata tcaattaact gaggtgacaa
    10741 aatggcatca tatgaaaaac gcggaagtac atggcgttat cgtatatcac taggaaaaga
    10801 cgcaaaaacg ggcaaatata aatatatttc aaactcaggt tttaaacgca aatcagacgc
    10861 taaacaccac gctgaaatgg tagaacgtca attaagaacc ggtgattata tcgcaccgtc
    10921 cacatctaca tttaaacagg ttgctaacga ttggcttaaa caatatgcta acgaggtaaa
    10981 agtaagtagt gtcagagcac gcgagaaagc catacaccac gccatagaac gctttaacaa
    11041 taaaccaata caaactatca ataaacatga ttatcaacgc tttgtaaatg atataagcgc
    11101 acagtatagc aagaattata ttgatagcat tatagcctct acgaatatga tatttaagta
    11161 cgcatatgat atgaaattaa taagaatact gcctagcgag ggtattaaac gacctaaaaa
    11221 gaaagttagt gtggaagaat tagaagatac ttagatacat aaaaagtttc ttgaaaaaga
    11281 tgaattattt caattcctgg aggttgctaa aaatcaccat tcaccccaaa acagctttga
    11341 ggtgttttgt acattagcat atacaggcat gcgtgcaggt gaattattgg cattgaagtg
    11401 gtctgatata gactttgaga ataacactat caatattaca aagacttatt acaatccaaa
    11461 taacaataaa aagcaatatc aaatacttac accaaaaact gaaagctcaa tcggaaaaat
    11521 ttcagtggat cctcatgtga ttaaattact taaaaattat aaaaccgacg tgcaggacac
    11581 atggaaaaac aaattgtatg tagataataa tttcgttttt actgatgtta acggctaccc
    11641 tcttgtaatt aaaaaactac aattatggat aaaagctata cttaaaaaga ctgacataac
    11701 taataaacaa ataagcactc attcatttcg tcatactcat tgtgcgttac ttatagaggc
    11761 tggtgtgcat attaaggaaa tacaagaacg cttgcgccat aaagatataa ataccactat
    11821 gaacatctac gctaagatta cgaactcata caaaaaagac gcttcccaaa agtttagtaa
    11881 actcatggaa aacgtctcaa aagatttatt ttaaaatttc tatgaccaaa ttatgaccac
    11941 ccaatattac aaactttata aaatcagcgt ttaacagtca ttttacatca ttcccggcat
    12001 gccacccatg ttaggttggt cattattttt ttctggaatt gatgctacaa ccgcttcagt
    12061 cgttaagaac attgctgcaa cacttgcagc atgttgtaat gctgagcgtg ttactttagt
    12121 tggatcaacg atacctgctt ctaacatatt aacccactcg tttgtagcag cgttaaaacc
    12181 aacacccggc tctgcgtttt tcaaacgttc tacaataaca gaaccttcta atcctgcatt
    12241 ttcagcaatt tgacgaactg gtgcagttag tgctttaagt acaatattta cacctgtttc
    12301 aatgtcacct tctgcttcaa tttcacttac tttttggtaa acatttacta atgcagtacc
    12361 accacctgca acaatacctt cttcaactgc tgcacgtgta gaatttaatg catcttcaat
    12421 acgtaattta cgttctttaa gctctgtttc acttgctgca cctactttaa taactgcaac
    12481 accacctgct aatttagcta agcgctcttg taatttttca cgatcaaagt cagattcagt
    12541 ttcttcaatt tgagatttca attggcttac acgtgcatca atgctgtttt cgtcaccgtc
    12601 accatcaaca acagtggtat tatctttagt tacttctact ttacttgcag tacctaacat
    12661 atcaattgat gcatctttta aatctaagcc taaatcatca gtaatcactt gcgcaccagt
    12721 taaaatagct aaatcttcta acattgcttt acgacgatca ccgaaaccag gcgcctttac
    12781 tgcaacagct gtaaatgtgc cacgcattcg gtttagcacg atatttgtta atgcatcgcc
    12841 ttcaacttca tcagctacaa ttaagattgg acgattagat tgaaccactt gttctaataa
    12901 aggtaagata tcttggaaag acgagatttt cttatctgtt actaaaatgt atgggcgttc
    12961 taattcagca accattttat ctgaatcagt aaccatatat ggtgattgat aaccacgatc
    13021 aaattgcata ccttcaacca cttctagttc agtgtttagt ccatttgatt cttcaattgt
    13081 aatgacacca tcgttaccta ctttttccat agcttcagaa atataacgtc caatttcttc
    13141 atctgctgct gaaatcgcac ctacttgcgc tatttcattt ttattttcaa ctttttgaga
    13201 attttcatgt aatgcttcta cagcaacttt aactgctttg tcgatacctt gacgtaaacc
    13261 aactgggttc gcaccacttg taacattttt caagccttct tgaatcattg cttgagctaa
    13321 tactgttgca gttgtcgtac cgtcaccagc aatttcattt gtcttatttg caacttcttg
    13381 aactagttta gcccccatat tttcatatgg atcttctaat tcgatttctt tagcaatcgt
    13441 tacaccatcg ttcgtaatta aaggtgctgt aaactcttta tctaatacaa cattacgtcc
    13501 tttaggacca atcgttactt taactgcatt tgctaattgg tctacaccac gtaacattgc
    13561 ttgacgtgca tcttcagaga atttcaattg tttaaccata aatgataaac ctccatttgt
    13621 ttagtcatat ttcattttcg ttctttacaa tttatctatg aatttaattt tatattattc
    13681 aattaccgct aaaatatctt cttcatttaa tactagatat gtttcattat ctcgtttaac
    13741 ttctgtacca gcatattgtt ggaacacgac acggtcccct tctttcactt caggagtcac
    13801 tcttgtacca tcatttaata gacgtccagt tcctactgca acgataacgc cttcgtttga
    13861 tttttcttta gcactatcag ttaaaacaat accactttta gttgtttgtt cttgttcttt
    13921 tttctcaata atcacacgat ttccaattgg ttttagcatg attgttcctc cttaaaaaac
    13981 ctaaagttta gcacttaaca ttaaagagtg ctaacataca tttataataa tcaaatttgg
    14041 tcaaaatttc aagtcataac cttttaatta agttttgtaa cttcaagtta tttacgataa
    14101 aataaattat aaacaaatat ttggaggaaa attatgacaa gattatgggc atcattgcta
    14161 actgttatta tttatatatt gtctcaattt ttaccgcttc tcattgtaaa aaaattacca
    14221 tttgtacaat atagtggcat agaactgact aaagcagtca tttacataca acttgttcta
    14281 tttttaatcg ccgccacgac gattatttta attaatttaa aaattaaaaa tccaacaaaa
    14341 ttagaattag aagttaaaga acctaaaaaa tatatcattc catgggcatt gcttggattt
    14401 gcattggtaa tgatttatca aatggtagtg agcattgtat taacgcaaat ttatggtgga
    14461 caacaagtaa gtcctaatac agaaaagcta attattattg ctcgaaaaat acctatattt
    14521 atcttctttg tatctattat cggtccttta ttagaagaat acgtattcag aaaagtaatc
    14581 tttggagaat tatttaatgc gattaaaggt aatcgtatcg tagcatttat tattgctaca
    14641 acagtaagtt cattaatatt tgcattagca cataatgatt tcaaatttat tccagtttat
    14701 tttggtatgg gcgtcatttt ttcattagca tatgtatgga caaaacggct tgctgttcca
    14761 attattatcc atatgttaca aaacggattt gtcgttatat tccaattact taatcctgaa
    14821 gctttgaaaa aagccacgga acaagcgaat tttatatatc acatttttat tccataatat
    14881 aaaaaacgaa tcacacacgg attatcgtcg ctgtgattcg tttttatttt tctttttatt
    14941 tcgctttaca aataagaata gcatgattga aaataccacc atcatagaac atacaattgc
    15001 tagccaactt ttactatatg cagttgacgc acttttctta ttatcttctt gttttttaac
    15061 tactgtaaaa tcgtcttttt catgcggttc aaatgagcgt tgttttgaat ccggcttatc
    15121 ttcgggcttt ttcaaatctg gcattgatcc aatttcacca gtaattaatg cgagtacttg
    15181 ttcatctaaa ttacgatagt acctttctga tttcaatgga ttgaaatatt ggaatctaaa
    15241 gtaattcgat tgctgttgta atccatttaa atatgcattt ccgctgaaat tttgttttcg
    15301 aataatatta taaatgtctt catcagtaac ttctccataa tctttgccca acttattaat
    15361 ttgatttaaa atatacggat tatacttgta agccccattt gccaaggcta aaaatcgttg
    15421 ggatacaaaa tcattaccag taggattttg tgagtttcct tgattcccat ttggttgcca
    15481 ttgaccttga ttagatccat ctgaagcatt tgggttccat gttcccccat ttttcgagcc
    15541 accagaatga tttgaatccc caggttgatc gggatctggt gacgggttag gatttggctt
    15601 attagggtct ggttttggat tcggatttgg ctttggttta tctggatctg gttttggatc
    15661 cggatttggc tttggtttat ctgggtctgg ttttggtttc gggttatctg gatctggttt
    15721 tggattcgga cttgggtttt ggttatttgg gtctggtttt gaatctgtac ttgggttttg
    15781 gttgtttgag tctggcttca cattattcga atcaggatta gcatcacgac tattcgaacc
    15841 tgttccttca ttacccgttt gatttgattc gtgaggtgtc ccagtctcat tattattcat
    15901 ttcactattt actgatggtg aagttatatt atgattcaaa ttagtagttg gtttttctcc
    15961 atttaagtta tcctttgctt caactgtgtg cgttgcaagc cctaaactac tactacttaa
    16021 aattaaaata cttgttgaaa gtaacatttt cttaaaccaa tgatatgaca tttcacgaga
    16081 cacctccctt tttcatgtaa gtattaatct aagtataata aatatgcgtt gaaaatgcat
    16141 ttatatttgg aatttgaaaa tgaatattaa tattcttgaa attttcgtaa atattacttt
    16201 acaagtccga gtataaattt tatacttagt ctattaagta ttctttacta aagaaggagc
    16261 aataattatg ggcttattta aaaaagataa aaaagcaatg acttttgaca atgcaatgga
    16321 agaaagacgt tcaatatata atttgaaaga ttcgatatca attagcgatg acgaattaga
    16381 atcaatgatt gcacatgcag ttaaacatgt tccttcatca tttaattctc aatcaactag
    16441 aattgttctc ttgttaaatg acaacaataa taaattttgg gataacacaa aagcaatttt
    16501 aaaagaagtc atgggagaga atcgtgattt cgagccaact gaacaaaaaa ttgataactt
    16561 caaacattca tacggtacaa ttttgtttta tgaagatcaa gatgttgtaa gtggtttaca
    16621 agaacaaatg ccaaactact atgataattt tgcaatatgg tcaactcaaa caaatgccat
    16681 gcatcaattt gcaatttgga ctgcattagc tactaaaggc ataggtgctt cattacaaca
    16741 ctataatcca cttgtcgatg agatgacttc aaaagaattt aatattccta aaagttggaa
    16801 acttattgct caaatgccat ttggtgatat tcgtgaagct gctggcgaaa aaacatttaa
    16861 tccagtggaa gatcgtttcg taataaaaaa ataatcaata tattgttaag aatgggacag
    16921 aaatgataaa gaatcattag ttattcatta tgtagtagtt cttacacatt agccgctgct
    16981 aatgtgcact taagaatagg aatacatgag taaaactcat gtataagaaa tactaatttc
    17041 taaagaaaaa gtatttcttt atgttagagc ccaccccaac ttgcattgtc tgtagaaatt
    17101 ggtgttccaa tttctctatg ttggggcccc atcaactact gtcaatataa cattgtagag
    17161 cctaggactt tgattttatg tcttgggctc gttttttgcg tttcaacata acttttagta
    17221 agttatatat aagatctatc aaggatgcga tgttatgaaa gtccaaattt atcaattacc
    17281 gattattttt ggagatagtt ctaaaaatga aactcaaata acgcaatggt tcgaaaaaaa
    17341 tatgaatgct gaagtagatg tagtcgtttt acctgaaatg tggaataatg gctatgattt
    17401 agagcattta aatgaaaaag cagataataa tctaggacaa agtttttcat ttattaaaca
    17461 tttagctgaa aaatataaag tcgatatcgt agctgggtca gtttctaaca ttagaaacta
    17521 tcaaatattt aatacagctt ttagtgtgaa taaaagtggc cagctgatta atgaatacga
    17581 caaagttcat ttagttccta tgttaagaga gcatgaattt ttaaccgctg gcgaaaacgt
    17641 agctgaaccc tttcaattgt ctgacggtac ttatgtgacc caacttatct gttatgattt
    17701 acgttttcca gaattattaa gatatcctgc tcgtagtggt gctaaaattg cattttatgt
    17761 ggcgcaatgg cctatgtcac gtcttcaaca ttggcattca ttactaaagg cacgtgcaat
    17821 tgaaaataat atgtttgtca ttggaactaa tagcactgga ttcgatggta acacagaata
    17881 tgctggtcat tcgatagtta taaacccaaa tggagattta gttggagaat taaatgaatc
    17941 cgcagatatt ttgactgtag atttaaactt aaatgaagta gaacagcaac gcgaaaatat
    18001 acctgtattt aaaagtatta aattagattt gtataaataa gaagcgcccg aaataatatt
    18061 taacacatgt catgactaaa catagattta tgagagattt ttcataagtc tatgtttatt
    18121 tttttataat agataactca gtaagaatcc atttcgccct ttgcaaatga atgattttct
    18181 taattaaatt gattaatttt ttgacctttt ccaacattag acttattcat atattttaac
    18241 ggtgggtctc ataatgagga ttaactctcc ccttctttat tacaataaaa aaggccgcga
    18301 gcttgggagg ggctcacgac catacttatt attaagggaa tgttttacag ttatttt
//
LOCUS       DANBWH010000033        18343 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.033, whole genome shotgun sequence.
ACCESSION   DANBWH010000033 DANBWH010000000
VERSION     DANBWH010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18343
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.033"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(158..1417)
                     /gene="femB"
                     /locus_tag="PPY92_000783"
     CDS             complement(158..1417)
                     /gene="femB"
                     /locus_tag="PPY92_000783"
                     /EC_number="2.3.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemB"
                     /protein_id="HDJ2624803.1"
                     /translation="MKFTELTVTEFDNFVQNPSLESHYFQVKENIVTRENDGFEVVLL
                     GIKDDNNKVIAASLFSKIPTMGSYVYYSNRGPVMDFSDLGLVDYYLKELDKYLQQHQC
                     LYVKLDPYWLYHLYDKDIVPFEGREKNDALVNLFKSHGYEHHGFTTEYDTSSQVRWMG
                     VLNLEGKTPEILKKTFDSQRKRNINKAINYGVKVRFLERDEFNLFLDLYRETEERAGF
                     VSKTDDYFYNFIDTYGDKVLVPLAYIDLDEYVLKLQQELNDKENRRDQMMAKENKSDK
                     QMKKIAELDKQIDHDQHELLNASELSKTDGPILNLASGVYFANAYEVNYFSGGSSEKY
                     NQFMGPYMMHWFMINYCFDNGYDRYNFYGLSGDFTENSEDYGVYRFKRGFNVQIEELI
                     GDFYKPIHKVKYWLFTTLDKLRKKLKK"
     gene            complement(1436..2698)
                     /gene="femA"
                     /locus_tag="PPY92_000784"
     CDS             complement(1436..2698)
                     /gene="femA"
                     /locus_tag="PPY92_000784"
                     /EC_number="2.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemA"
                     /protein_id="HDJ2624804.1"
                     /translation="MKFTNLTAKEFGAFTDSMPYSHFTQTVGHYELKLAEGYETHLVG
                     IKNNNNEVIAACLLTAVPVMKVFKYFYSNRGPVIDYENQELVHFFFNELSKYVKKHRC
                     LYLHIDPYLPYQYLNHDGEITGNAGNDWFFDKMSNLGFEHTGFHKGFDPVLQIRYHSV
                     LDLKDKTADDIIKNMDGLRKRNTKKVKKNGVKVRYLSEEELPIFRSFMEDTSESKAFA
                     DRDDKFYYNRLKYYKDRVLVPLAYINFDEYIKELNEERDILNKDLNKALKDIEKRPEN
                     KKAHNKRDNLQQQLDANEQKIEEGKRLQEEHGNKLPISAGFFFINPFEVVYYAGGTSN
                     AFRHFAGSYAVQWEMINYALNHGIDRYNFYGVSGKFTEDAEDAGVVKFKKGYNAEIIE
                     YVGDFIKPINKPVYAAYTALKKVKDRIF"
     gene            complement(3020..3748)
                     /locus_tag="PPY92_000785"
     CDS             complement(3020..3748)
                     /locus_tag="PPY92_000785"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDJ2624805.1"
                     /translation="MTKLFIPYIMGNKDLIENATLLSENGADIIEIGVPFSDPVADGP
                     VIMEAGQQAIKQGITIDYIFNQLEKHGDQIKCNYVLMTYYNIICHYGEQAFFEKCRDT
                     GVYGLIIPDLPYELSQRLKQQFSHYGVKIISLVAMTTDDKRIKDIVSHAEGFIYTVTM
                     NATTGQNGAFHPELKRKIESIKAIANVPVVAGFGIRTPQHVADIKEVADGIVIGSEIV
                     KRFKSNTREEIIRYLQSIQQTLNN"
     gene            complement(3741..4955)
                     /gene="trpB"
                     /locus_tag="PPY92_000786"
     CDS             complement(3741..4955)
                     /gene="trpB"
                     /locus_tag="PPY92_000786"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDJ2624806.1"
                     /translation="MNKQIQTEADELGFFGEYGGQYVPETLMPAIIELKKAYKEAKAD
                     PEFQRELEYYLSEYVGRATPLTYAASYTESLGGAKIYLKREDLNHTGAHKINNALGQA
                     LLAKRMGKKKLVAETGAGQHGVASATVAALFDMELVVFMGSEDIKRQQLNVFRMELLG
                     AKVVAVEEGQGTLSDAVNKALQYWVSHVDDTHYLLGSALGPDPFPTIVRDFQSVIGKE
                     IKSQILKKEGRLPDAIVACIGGGSNAIGTFYPFIKDDVALYGVEAAGQGEDTDKHALA
                     IGKGSPGVLHGTKMYLIQDEGGQVQLAHSISAGLDYPGIGPEHSYYHDIGRVTFENAS
                     DTQAMNALINFTKHEGIIPAIESAHALSYVERLAPTMSKEDIIVVTISGRGDKDMETI
                     RQYMAERGLAND"
     gene            complement(4948..5580)
                     /locus_tag="PPY92_000787"
     CDS             complement(4948..5580)
                     /locus_tag="PPY92_000787"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HDJ2624807.1"
                     /translation="MKLKFCGFTSIKDVTAASQLPIDAIGFIHYEKSKRHQTITQIKK
                     LASAVPNHIDKVCVMVNPDLTTIEHVLSNTSINTIQLHGTESIDFIQEIKKKYSSIKI
                     TKALAADENIIQNINKYKGFVDLFIIDTPSVSYGGTGQTYDWTILKHIKDIPYLIAGG
                     INSENIQTVNQLKLSHQGYDLASGIEVNGRKDIEKMTAIVNIVKGDRDNE"
     gene            complement(5580..6362)
                     /gene="trpC"
                     /locus_tag="PPY92_000788"
     CDS             complement(5580..6362)
                     /gene="trpC"
                     /locus_tag="PPY92_000788"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HDJ2624808.1"
                     /translation="MTILAEIVKYKQSLLQNGYYQDKLNTLKSVKIQNKKSFINAIEK
                     EPKLAIIAEIKSKSPTVNDLPERDLSQQISDYEKYGANAVSILTDEKYFGGSFERLQA
                     LTTKTTLPVLCKDFIIDPLQIDVAKQAGASMILLIVNILSDKQLKDLYNYAISQNLEV
                     LIEVHDRHELERAYKVNAKLIGVNNRDLKRFVTNVEHTNTILENKKPNHHYISESGIH
                     DASDVRKILHSGIDGLLIGEALMRCDNLSEFLPQLKMQKVKS"
     gene            complement(6364..7362)
                     /gene="trpD"
                     /locus_tag="PPY92_000789"
     CDS             complement(6364..7362)
                     /gene="trpD"
                     /locus_tag="PPY92_000789"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HDJ2624809.1"
                     /translation="MTLLTRIKTETILLESDIKELIDILISPSIGTDIKYELLSSYSE
                     REIQQQELTYIVRSLINTMYPHQPCYEGAMCVCGTGGDKSNSFNISTTVAFVVASAGV
                     KVIKHGNKSITSNSGSTDLLNQMNIQTTTVDDTPNQLNEKDLVFIGATESYPIMKYMQ
                     PVRKMIGKPTILNLVGPLINPYHLTYQMVGVFDPTKLKLVAKTIKDLGRKRAIVLHGA
                     NGMDEATLSGDNLIYELTEDGEIKNYTLNATDYGLKHAPNSDFKGGSPEENLAISLNI
                     LNGKDQSSRRDVVLLNAGLSLYVAEKVDTIAEGIELATTLIDNGEALKKYHQMRGE"
     gene            complement(7368..7934)
                     /locus_tag="PPY92_000790"
     CDS             complement(7368..7934)
                     /locus_tag="PPY92_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDJ2624810.1"
                     /translation="MILVVDNYDSFTYNLVDIVAQHTDVIVQYPDDDNVLNQSVDAVI
                     ISPGPGHPLDDQQLMKIISTYQHKPILGICLGAQALTCYYGGEVIKGDKVMHEKVDTL
                     KVISHHQHLLYQDIPEQFSIMRYHSLISNPDNFPEELKITGRTEDCIQSFEHKERPHY
                     GIQYHPESFATDYGVKIITNFINLVKEG"
     gene            complement(7931..9337)
                     /locus_tag="PPY92_000791"
     CDS             complement(7931..9337)
                     /locus_tag="PPY92_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDJ2624811.1"
                     /translation="MDIFYKKIKANVTPEVLAQLHSKKIILESTNQQQTKGRYSVVIF
                     DIYGTLTLDNDVLSVSTLKESYQIIERPYHYLTTKINEDYHNIQDEQLKSLPFISGYV
                     GTCSFDLVRHEFPKLQSIQLEDHKQHDVRLYMVEQVYVFDHYKDELYIIATNQFSNST
                     KSDLENRVNKSIEDLTKIQPFMPTQDFDFKTKEIQSNISEERFIEMIQYFKEKITEGD
                     MFQVVPSRIYKYAHHASQHLNQLSFQLYQNLKRQNPSPYMYYLNIDQPYIVGSSPESF
                     VSVKDQIVTTNPIAGTIQRGETTQIDNENMKQLLNDPKECSEHRMLVDLGRNDIHRVS
                     KIGTSKITKLMVIEKYEHVMHIVSEITGKINQNLSPMTVIANLLPTGTVSGAPKLRAI
                     ERIYEQYPHKRGVYSGGVGYINCNHNLDFALAIRTMMIDEQYINVEAGCGVVYDSIPE
                     KELNETKLKAKSLLEVRP"
     gene            complement(9829..10861)
                     /locus_tag="PPY92_000792"
                     /pseudo
     CDS             complement(9829..10861)
                     /locus_tag="PPY92_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499892.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
     gene            11026..12117
                     /locus_tag="PPY92_000793"
     CDS             11026..12117
                     /locus_tag="PPY92_000793"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDJ2624812.1"
                     /translation="MTTVLFVGLGLIGGSLASNIKYHNPNTNIIAYDADTSQLDKAKS
                     IGIINEKCLNYSEAIKKADVIIYATPVAITNKYLSELIDMPTKPGVIVSDTGSTKAMI
                     QQHESNLLKHNIHLVSGHPMAGSHKSGVLNAKKHLFENAYYILVYNEPRNEQAANTLK
                     ELLSPTLAKFIVTTAEEHDYVTSVVSHLPHIVASSLVHVSQKNGQEHHLVNKLAAGGF
                     RDITRIASSNAQMWKDITLSNKTYILEMIRQLKSQFQDLERLIESNDSEKLLSFFAQA
                     KSYRDALPAKQLGGLNTAYDLYVDIPDESGMISKVTYIMSLHNISISNLRILEVREDI
                     YGALKISFKNPTDRERGMQALSDFDCYIQ"
     gene            complement(12254..13516)
                     /locus_tag="PPY92_000794"
     CDS             complement(12254..13516)
                     /locus_tag="PPY92_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDJ2624813.1"
                     /translation="MYNYHLLEDRDVLCIDQKSFFASVSCIEKGLDPLETKLAVVADT
                     KRQGSVILAATPKLKELGIKTGSRLFEIPHRNDIYIINPSMRKYLNVSVAISKIALRY
                     IPPEDLHQYSIDEFFMDVTDSYHRFSSTVHAFCERLKREIYEETGIYCTVGIGSNMLL
                     SKIAMDVEAKHSQNGIAEWRYQDVPTKLWPIQPLRDFWGINRRTEAKLNKRGIFTIGD
                     LAKYPYKFLKKEFGILGVDMHLHANGIDQSKVREKHKISNPSICKSQILMRDYHFDEA
                     KVVMQELIEDVASRVRARKKVARTIHFAFGYSDEGGVHKQYTLKDPTNLEKDIYKVVM
                     HFADKLCNKQALYRTLSISLSQFINEDERQLSLFEDEYQRKRDECLAKTIDQLHLKYG
                     KGIVSKAVSFTEAGTKHGRLGLMAGHKM"
     gene            13664..13849
                     /locus_tag="PPY92_000795"
     CDS             13664..13849
                     /locus_tag="PPY92_000795"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HDJ2624814.1"
                     /translation="MPIVNVKLLEGRSDEQLKNLVSEVTDAVEKTTGANRQAIHVVIE
                     EMKPNHYGVAGVRKSDQ"
     gene            complement(13937..14920)
                     /locus_tag="PPY92_000796"
     CDS             complement(13937..14920)
                     /locus_tag="PPY92_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDJ2624815.1"
                     /translation="MDKETNDNEYRRQSEHRTSAPKRKKKKKIRKLPIILLIVVILLI
                     ALVVYIVHSYNSGVEYAKKHAKDVKVHQFNGPVKNDGKISILVLGADKAQGGQSRTDS
                     IMVVQYDFINKKMKMMSVMRDIYADIPGYGKHKINSAYALGGPELLRKTLDKNLGINP
                     EYYAVVDFTGFEKMIDELMPEGVPINVEKDMSKNIGVSLKKGNHRLNGKELLGYARFR
                     HDPEGDFGRVRRQQQVMQTLKKEMVNFRTVVKLPKVAGILRGYVNTNIPDSGIFQTGL
                     SFGIRGEKDVKSLTVPIKNSYEDVNTNTDGSALQINKNTNKQAIKDFLDED"
     gene            15057..15566
                     /gene="msrA"
                     /locus_tag="PPY92_000797"
     CDS             15057..15566
                     /gene="msrA"
                     /locus_tag="PPY92_000797"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDJ2624816.1"
                     /translation="MNINTAYFAGGCFWCMTKPFDTFDGIEKVTSGYMGGHIENPTYE
                     QVKSGTSGHLETVEIQYDVALFSYNKLLEIFFSVIDPLDTGGQYQDRGPQYQTAIFYT
                     NDHQKELAETYIEQLKNTINADKAIATKILPASQFYKAEDYHQDFYKKNPERYAEEQK
                     IRQEYKNKQ"
     gene            complement(15769..18291)
                     /gene="mprF"
                     /locus_tag="PPY92_000798"
     CDS             complement(15769..18291)
                     /gene="mprF"
                     /locus_tag="PPY92_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDJ2624817.1"
                     /translation="MNQEVKNKIFSILKITFATALFIFVAITLYRELSGINFKDTLVE
                     FSKINRMSLVLLFIGGGASLVILSMYDVILSRALKMDISLGKVLRVSYIINALNAIVG
                     FGGFIGAGVRAMVYKNYTHDKKKLVHFISLILISMLTGLSLLSLLIVFHVFDASLILD
                     KITWVRWVLYVVSFFLPLFIIYSMVRPPDKNNRFVGLYCTLVSCVEWLAAAVVLYFCG
                     VIVDAHVSFMSFIAIFIIAALSGLVSFIPGGFGAFDLVVLLGFKTLGVPEEKVLLMLL
                     LYRFAYYFVPVIIALILSSFEFGTSAKKYIEGSKYFIPAKDVTSFLMSYQKDIIAKIP
                     SLSLAILVFFTSMIFFVNNLTIVYDALYDGNHLTYYILLAIHTSACLLLLLNVVGIYK
                     QSRRAIIFAMISILLITVATFFTYASYILITWLAIIFVLLIVAFRRARRLKRPVRMRN
                     IVAMLLFSLFILYVNHIFIAGTLYALDIYTIEMHTSVLRYYFWLTILIIAIIIGMIAW
                     LFDYQFSKVRISSKIEDCEEIINQYGGNYLSHLIYSGDKQFFTNENKTAFLMYRYKAS
                     SLVVLGDPLGDENAFDELLEAFYNYAEYLGYDVIFYQVTDQHMPLYHNFGNQFFKLGE
                     EAIIDLTQFSTSGKKRRGFRATLNKFDELNISFEIIEPPFSTEFINELQHVSDLWLDN
                     RQEMHFSVGQFNEEYLSKAPIGVMRNEENEVIAFCSLMPTYFNDAISVDLIRWLPELD
                     LPLMDGLYLHMLLWSKEQGYTKFNMGMATLSNVGQLHYSYLRERLAGRVFEHFNGLYR
                     FQGLRRYKSKYNPNWEPRFLVYRKDNSLWESLSKVMRVIRHK"
BASE COUNT         5886 a         3347 c         2618 g         6492 t
ORIGIN      
        1 ataaaactaa atcaataaaa catcattata atgaaaaaca ttagattgac aaatacatac
       61 tagtttttct tagtgtgaaa taattaaaat ttcgctaaaa aaagcataaa aaagagccta
      121 tagtatcaat aactataagc cctaacatca tttacatcta tttctttaat tttttacgta
      181 atttatccaa tgttgtgaac aaccaatatt tcactttatg aattggttta tagaaatccc
      241 ctattaattc ttcgatttgt acattaaatc cacgtttaaa gcggtatacg ccataatctt
      301 cactgttttc cgtaaaatca cctgataaac catagaaatt ataacgatca tagccattat
      361 cgaagcaata gttaatcata aaccaatgca tcatgtatgg tcccataaat tgattatatt
      421 tttctgatga accaccagag aaataattca cttcatatgc atttgcaaaa taaacgccag
      481 aagcaaggtt tagaattggg ccgtccgttt tgctcaattc acttgcattc aataattcat
      541 gctgatcatg atcaatttgc ttatctaatt ctgcaatttt cttcatttgt ttatctgatt
      601 tgttttcttt cgccatcatt tgatcacgac gattttcttt gtcattcaat tcctgttgca
      661 actttaacac atattcatca aggtcaatat atgctaatgg tactaatact ttatctccat
      721 atgtgtcaat aaagttataa aaataatcat ctgtttttga cacaaatcca gcacgctctt
      781 cagtttcacg atataaatct aagaaaagat tgaactcatc acgttcaagg aatctgactt
      841 taacaccata gtttatcgct ttattaatat tacgtttacg ttgactatca aatgtctttt
      901 tcaatatttc gggtgtttta ccttcaaggt ttaatacgcc catccatcgt acttggctcg
      961 atgtatcata ctcagttgta aagccatgat gctcgtaacc atgtgattta aacaagttta
     1021 ctaaggcatc atttttctcg cgaccttcaa atggcacgat atctttatca tatagatgat
     1081 ataaccaata cggatctaat ttaacatata aacattgatg ttgctgtaaa tatttatcta
     1141 actcttttaa ataataatca actaatccta aatctgaaaa atccattact ggaccacgat
     1201 tcgaatagta aacataactt cccatagtag gaattttaga gaaaaggctt gctgcaatta
     1261 ctttgttatt gtcgtcttta atacctaata aaactacttc aaagccatca ttctcacggg
     1321 taactatatt ttcttttact tggaaataat gactttccaa tgatggattt tgtacaaagt
     1381 tgtcaaattc ggtaacagtt aactctgtaa atttcatgtt ttgataattc ccttcctaaa
     1441 aaattctgtc tttaactttt ttaagtgcgg tatatgctgc gtaaacaggt ttattaattg
     1501 gtttaataaa gtcaccaaca tattcaataa tttcagcatt gtaacctttt ttgaatttaa
     1561 ctacaccagc atcttcagca tcttctgtaa atttaccact aacaccatag aaattataac
     1621 ggtcaatgcc atgatttaat gcataattaa tcatttccca ttgcactgca taacttccgg
     1681 caaaatgacg gaatgcattt gatgtaccac cagcataata aacaacttca aatgggttga
     1741 taaagaagaa accagcagag ataggtaatt tattaccatg ttcttcttgt agacgtttac
     1801 cttcttcaat cttttgctca ttagcatcaa gttgttgttg taagttatct cgcttgttgt
     1861 gtgctttttt attttcagga cgtttttcaa tatcctttaa cgctttattt aaatctttat
     1921 ttaaaatatc acgctcttcg tttagttctt taatatattc atcaaagttg atatacgcta
     1981 aaggtactaa cacacggtct ttgtaatatt ttaagcgatt gtagtaaaat ttgtcatcac
     2041 gatcagcaaa agcttttgat tctgacgtat cttccataaa tgatctaaaa attggcagtt
     2101 cttcttcaga taaatatctt actttaacac cattcttttt aacttttttc gtgtttcttt
     2161 ttctaagtcc atccatattt ttaatgatgt catctgctgt tttatctttt aaatctaaca
     2221 ctgagtgata acgaatttgt agcacaggat caaatccttt atggaatcca gtatgttcaa
     2281 atcctaagtt actcatttta tcaaagaacc aatcattacc agcattacct gtaatctcgc
     2341 catcatgatt caagtattga tatggtaaat atggatcgat atgtaggtat agacaacgat
     2401 gttttttaac atattttgat aattcattaa agaaaaagtg tacgagttct tgattttcat
     2461 aatcgatcac tggaccgcga tttgaataaa aatacttgaa cactttcata acaggtacag
     2521 cagtaagtaa gcaagctgca atgacctcgt tattattgtt ttttattccc actaaatgtg
     2581 tttcataacc ttcagcaagc tttaactcat agtggccaac agtttgcgtg aaatgactgt
     2641 atggcatgct atctgtaaag gcaccaaact ctttagctgt taaatttgta aacttcatta
     2701 tcattactcc tatttgtctc tcgttaatta atttcatttc cgtatttgca gtttttctat
     2761 ttcccctctg caaatgtcaa aaataataaa tctaatctaa ataagtatac aatagttaat
     2821 gttaaaacta aaacataaac gctttaattg cgtatacttt tatagtaata tttagatttt
     2881 tgcatacaat ttcaaaaaaa gtaatatgaa cgtttgggtt tgctcatatt acttttgtat
     2941 catttctatt caattttata attcaccgtt tttcactttt tcaaacagta ttcgcctaat
     3001 ttttttaaat caagtaaact taattattca atgtttgttg gatagattgt aaatatctaa
     3061 tgatttcctc acgcgtgtta gatttaaatc gcttaacgat ttcgctacca atgacgatgc
     3121 catctgcaac ctcttttata tctgcaacat gttgtggtgt tcttatacca aatcctgcga
     3181 caactggcac attggctatc gctttaattg actcaatttt tcgttttaat tctggatgaa
     3241 acgcaccgtt ttgtcctgtt gtcgcattca tcgtcacagt ataaataaag ccttccgcat
     3301 gagatacgat atcttttata cgtttgtcat cagtagtcat cgcaactaac gatatgattt
     3361 tgacgccata gtgactaaat tgttgtttta aacgctgcga taattcatat ggtaaatcag
     3421 gaataattaa gccgtagaca ccagtatctc gacatttttc aaaaaacgct tgttctccat
     3481 aatgacaaat aatattataa tacgtcatta atacatagtt acacttaatt tgatcaccat
     3541 gtttttctaa ttgattgaaa atataatcta tcgtgatgcc ttgtttaatc gcttgttgac
     3601 ctgcttccat gataactgga ccatcagcaa ccggatcaga gaaaggtaca ccaatttcaa
     3661 ttatatctgc accattttca ctcaacaatg ttgcattttc aatcaaatct ttattgccca
     3721 taatataagg tataaataat ttagtcattt gcaagacctc gctctgccat atattgtcta
     3781 attgtttcca tatctttatc gccacgtcca gaaatagtta ctacaataat atcttctttc
     3841 gacatcgtag gcgctagtct ttcaacataa ctcagtgcat gtgcactttc aattgcaggt
     3901 ataatacctt catgttttgt aaagttgatt aaagcattca ttgcttgtgt atcacttgca
     3961 ttttcaaaag ttactctacc aatgtcgtgg taataagaat gttcaggccc aataccagga
     4021 taatcaagtc ctgctgaaat agaatgtgct agttgcactt gcccaccttc atcttgaatt
     4081 aaatacattt tagtaccatg taatacgcca ggtgatcctt tgccaattgc aagtgcatgt
     4141 ttatcagtat cctcgccttg acctgcggct tcaacaccgt ataatgcaac atcatcttta
     4201 ataaatggat aaaatgtacc gattgcattt gagccaccac cgatacatgc tacaattgca
     4261 tccggaagtc gaccttcttt cttcaatatc tgtgatttta tttctttacc aatgacactc
     4321 tgaaaatctc taacaatcgt tgggaatggg tctggaccta atgcagaacc taataaataa
     4381 tgtgtatcat ctacatgact tacccaatat tgcaatgctt tattaactgc atccgataaa
     4441 gtcccttgac cttcttcaac tgccacaacc tttgcaccaa gtaattccat tctaaataca
     4501 ttaagttgtt gtcttttaat atcttcactt cccataaaga caacaagttc catatcaaat
     4561 aatgcagcaa ccgtagcact agctacacca tgttgacccg caccagtttc agcaacaagc
     4621 ttcttcttgc ccattctttt agcaagcaac gcttgaccta acgcattatt aattttatgg
     4681 gcgcctgtat gatttagatc ctctcgtttc aaatatattt tagcgccacc taggctttca
     4741 gtatatgatg cagcatatgt aagtggtgtc gcgcgtccta catactctga taaatagtat
     4801 tccagttctc tttgaaactc tgggtctgct tttgcctctt tataagcttt tttcaactca
     4861 ataattgctg gcattaatgt ttctggaaca tattgccctc catattcacc aaagaaacct
     4921 aattcatctg cttctgtttg tatttgttta ttcattgtct ctatctcctt tcacaatatt
     4981 tacaattgct gtcatttttt ctatatcttt tcgcccattt acttctatac ctgatgcaag
     5041 atcataacct tgatgtgata atttaagttg attaactgtt tgaatatttt cagagttaat
     5101 gcctcctgct atcaaataag gtatgtcttt tatgtgcttc aaaatagtcc agtcatatgt
     5161 ttgaccggta ccaccatacg acactgaggg tgtgtcgata ataaataaat ctacgaaccc
     5221 tttatattta tttatgtttt ggattatgtt ttcatctgca gctaaagctt tagtgatttt
     5281 aatgcttgaa tatttctttt taatttcctg tataaaatca atagattctg tgccgtgtaa
     5341 ctgtattgtg ttaattgacg tattgcttaa tacgtgttca attgttgtta aatcaggatt
     5401 taccatgaca catactttat cgatatgatt tggaacagca gacgctaact tttttatttg
     5461 ggtaattgtt tgatgccttt tacttttttc ataatggatg aaacctatcg catcaatagg
     5521 taattgactg gccgctgtaa catcctttat tgatgtaaag ccacaaaatt tcaatttcat
     5581 catgacttca ccttttgcat tttcagttgt ggtaaaaatt cagatagatt gtcacaacgc
     5641 ataagcgcct cacctattag taagccatcg ataccactat gcaagatttt tcttacatca
     5701 gatgcatcgt gaataccact ttcagaaata taatgatgat tgggtttttt attttctaaa
     5761 atagtatttg tatgttccac atttgtgaca aatcgtttta agtccctgtt atttacacca
     5821 atcaatttag cattaacctt ataggcacgt tctaattcat ggcgatcatg tacttcaatt
     5881 aacacttcta gattttgcga tatagcgtag ttatataaat ctttcaattg tttatccgat
     5941 aagatgttaa cgatcaataa aatcatagat gcaccagctt gtttagcaac atcaatttga
     6001 agcgggtcta taataaagtc tttgcataat acgggtaatg ttgtttttgt tgtcaatgct
     6061 tgtaatcttt caaaactacc accaaagtac ttttcatcag ttaaaatgga cacggcattt
     6121 gcaccgtact tttcataatc tgagatttgt tgcgataaat ctcgttcagg taagtcatta
     6181 actgtaggac tcttcgattt aatttctgca ataattgcta gctttggttc tttctcaatt
     6241 gcgtttataa aagatttttt attctgaatc ttcacacttt tcaaggtatt aagtttgtct
     6301 tgataatagc cattttgtaa aagtgactgt ttatatttaa caatttctgc taaaatcgtc
     6361 atattattca cctctcattt gatggtattt tttcaatgct tcaccattat caatcaatgt
     6421 agttgcaagt tctatgcctt ctgcgatggt atccactttc tctgcaacat aaaggcttaa
     6481 acccgcattt agtaagacaa catcacgtcg acttgactga tctttaccat tcaagatatt
     6541 aagggagatt gctaaatttt cttcaggtga accgccttta aaatcactat tcggcgcatg
     6601 tttcaaacca taatcagtcg catttaatgt gtaatttttg atttctccat cttcagtcaa
     6661 ttcatatatc aaattatcac cagatagtgt tgcttcatcc ataccatttg caccatgtaa
     6721 aacgattgca cgttttctac ctaaatcttt aatcgtttta gcaactaact ttaactttgt
     6781 aggatcaaag acgcctacca tttgatacgt taagtgatat ggattaatta atggacccac
     6841 aaggtttaat attgtaggct ttccaatcat ttttctaact ggttgcatat acttcatgat
     6901 tggatatgat tcagttgcac caatgaatac aaggtctttt tcatttaatt ggttaggtgt
     6961 atcatcaaca gttgttgttt gtatgttcat ttgatttaac aaatccgtac tacctgaatt
     7021 tgaggtaata cttttattac catgttttat aacttttacg ccagcacttg ctacaacaaa
     7081 agcaacagtc gttgaaatgt tgaaactatt tgacttgtca ccacctgtgc cgcacacaca
     7141 catagcccct tcataacatg gttgatgtgg atacattgta ttaattaagc tacgtacaat
     7201 atatgttaat tcttgttgtt ggatttctcg ctccgaatag gaactaagta attcatattt
     7261 aatatcagtt ccaatactag gagaaataag tatatcgatt agctctttaa tgtcgctttc
     7321 aagtaatata gtttcagttt ttattcttgt tagtaatgtc atggttttca tccttccttc
     7381 actagattaa tgaaatttgt aattattttg acaccatagt ctgtagcaaa tgattcagga
     7441 tggtactgaa taccgtaatg cggtctttct ttatgctcaa atgactgtat acaatcttcg
     7501 gtacgtccag taattttcaa ttcttctgga aaattgtcag ggttacttat taatgaatga
     7561 tatctcataa ttgaaaactg ttctggtata tcttgatata acagatgttg atgatgcgat
     7621 ataaccttta gtgtatcaac tttttcgtgc ataaccttgt cgcctttaat gacttctcca
     7681 ccgtaataac aagtcagtgc ctgagcacct aggcaaatac ctaaaatggg tttgtgttga
     7741 taggttgata tgattttcat taactgttga tcgtctaatg gatgccctgg accaggagat
     7801 ataataacag cgtccaccga ttgattcagc acattatcat catcaggata ttgaacaatg
     7861 acgtcagtat gttgagcaac aatatccact aggttatatg taaaggaatc ataattatct
     7921 acaactaaga tcatggcctc acctccaata agcttttagc tttcaatttc gtttcattca
     7981 gttctttttc aggaatagaa tcatatacaa cgccacaacc agcttctacg ttgatatact
     8041 gctcatctat catcatcgtt cgaattgcta aagcaaaatc taagttatga ttacaattta
     8101 tgtatccaac accaccacta taaacgcctc gtttatgtgg atattgttca tatattcttt
     8161 caattgcacg taattttggt gcacctgaaa cggtacctgt tggtaataaa ttcgcaataa
     8221 ctgtcattgg tgataaattt tgatttattt tacctgtgat ttcacttacg atatgcataa
     8281 catgttcata tttttcaata accattaatt tagtaatttt tgaggtaccg attttactta
     8341 ctctatgaat atcattacgt cctaaatcaa ctagcatacg atgttcgctg cattcttttg
     8401 gatcattaag tagttgtttc atattctcat tatctatttg tgtcgtctca ccacgttgaa
     8461 tcgtacctgc aataggatta gttgttacaa tttgatcttt gacacttacg aaactttcgg
     8521 gagaactacc gacaatatat ggttgatcga tattaagata atacatatat ggacttgggt
     8581 tttgtcgttt taaattttga tacagttgaa acgaaagttg atttaaatgc tgactagcat
     8641 gatgcgcata tttgtaaatt cttgatggca caacttggaa catatcccct tctgttattt
     8701 tctctttgaa atactgaatc atttcgataa atctttcttc agaaatattt gattgaattt
     8761 ctttagtttt aaaatcaaaa tcttgtgtag gcatgaatgg ttggatttta gttaagtctt
     8821 cgatagactt attaactcga ttctcaagat ctgattttgt tgaatttgaa aattgattcg
     8881 tcgcgatgat atataactca tctttgtaat ggtcaaatac ataaacttgt tcaaccatat
     8941 ataaccttac atcgtgctgc ttgtgatctt ctaattgtat tgattgcaat ttaggaaatt
     9001 catgtcttac taaatcaaag ctacacgtcc caacatatcc agatataaac ggtaatgact
     9061 taagttgctc atcttgaata ttatggtagt cttcatttat tttagtcgtt aaataatggt
     9121 acggtctttc aatgatttga tacgattctt ttaaagtgct tactgataat acatcattat
     9181 ctaaagttaa agtgccataa atatcaaaaa taacaactga atagcgacct ttagtttgtt
     9241 gttgatttgt actttccaaa atgatcttct tggaatgaag ttgtgctaaa acttcgggcg
     9301 ttacatttgc ttttattttt ttataaaata tatccattct atacgctcct ttcaaaaaag
     9361 cgccagcatc cttatcaata taaaggacgc tgacgcgtgg taccacctaa attaaccgaa
     9421 tattcaaaat attcatagtt cactctttac tatatataat ttttctgttg cagacaaaac
     9481 ccaatttcat taatctcggt gttagttcac atcatccact aactttctgt tgctaccttt
     9541 aattaactac tacattttgt ctaaataaaa agacaccaca taacgcatat acctgtaagg
     9601 acgcgttacc gtggtgccac cttagttaac ataattcatg ttcactttca aaactcattc
     9661 aattgttttc caaagtccaa ttcacaagat atggcgtgct aattcccatc acccattagc
     9721 tttctatttg ctacgtttca tatctgctac tatttcttta gaaaattcta attattttta
     9781 tagttcatta tattacatag gtcgttcaca tttgtcaaca ctaattgttt actcaattgg
     9841 tgataagcaa tatgcatata gtaatttctc tgtcgcttta atagaatcaa tatgtgttcg
     9901 ttccattgca tgagatgatt caatgccagc gccaaataaa ccatgtctga tatccgcacc
     9961 agcatgtaaa gctgctgaag catctgaacc ataatatgga tatatgtcta ctttatatgg
    10021 aatgttattt attttgcaaa gattaactag gtgcgatttc aattgcttat gatatggacc
    10081 tgaagcatct ttggcacaaa tagaaactgt atattcatcc gatgcttgac cgtctcccaa
    10141 cgcgcccata tctaatgcaa tatattcttt gattttcgaa tcaattgatg catttgcacc
    10201 gtaacctatt tcttcgttat tagaaatata aaattgcgtt gtatgtggta atattatttg
    10261 ctcttctttt aatttcttta gtaattgtag tatcatcgct acgctagctt tatcatctaa
    10321 atgacgagat ttaataaaac ctgatgacgt gataactgta cgtggatcaa agctaacaaa
    10381 atcacctact gaaataccta aactctttgt atcttcttct gatgtagtta cttcatcaat
    10441 tcttatttcc atatgctttt gatctctagg tatttcatga ttatttctat atacatgaac
    10501 acttgtttca tgcagacaaa ttgttcctgt atatatttga ccagcatcag ttttaatttg
    10561 gcaatattca ccctcaatcg cgttatacgt gaatccctcc aattaattct attgctaagc
    10621 gaccatcttc tttaatttct ttaaccattg cacctaaagt atcaacatga gcagtaatac
    10681 agcgttgtac ttcatcattt ttgcctggca ctgttattaa taacgcacct ttatttgtta
    10741 gtgttgtttg ataacccaag tcttttgcat atttttcgac aaattgaatt gcttcttctg
    10801 tgtctcctga aggactatta atacctgtta atgttttaat catttctaat attggttcca
    10861 ttgatttacc cctccgttgc cattgtgtta ctatactctt tattgtagat gatttatctc
    10921 cgtttatcta ttaatgctat tatcgatttt tatttactat tgcactgtgt caacagctca
    10981 tttacaatgt tattattctg aaaatttcga aataaggtga tttatatgac aacagtttta
    11041 tttgttggac ttggattaat tggtggaagt cttgctagca atataaaata ccataaccct
    11101 aatactaata ttattgcata cgatgcagat acttctcagt tagataaagc taaatcaatc
    11161 ggcattatta atgaaaaatg tttaaattat agtgaagcta ttaaaaaagc cgatgtaatt
    11221 atttatgcaa cacctgttgc tatcacaaat aaatatctta gcgagcttat agatatgcca
    11281 actaaacctg gtgttattgt ttctgatact ggtagtacta aagcaatgat acagcaacac
    11341 gaatccaatt tattaaagca taatattcat ttagtcagtg gtcatccaat ggctggtagt
    11401 cataaatctg gtgtactaaa tgctaaaaag cacttatttg aaaacgctta ttatatttta
    11461 gtctataatg agccaagaaa tgagcaagca gcaaacacgt taaaagaact gttatcacct
    11521 actcttgcta aatttattgt aactactgct gaagaacacg actacgtaac aagcgtcgta
    11581 agtcatttac ctcatatcgt tgcatctagt ttagttcatg ttagtcaaaa gaacggtcaa
    11641 gaacatcatt tagttaataa acttgcagct ggtggttttc gtgatatcac tcgtatagct
    11701 agtagtaatg cacaaatgtg gaaagatatc accttgagta ataaaacgta tattttagaa
    11761 atgattcgac agctaaaaag tcagtttcaa gatttagaaa gactaattga aagcaatgat
    11821 tctgaaaaat tgttatcatt ttttgcccaa gctaaatcgt atcgcgacgc actacccgct
    11881 aaacaactag gtggactaaa tactgcgtat gatctatatg tagatattcc ggatgaatca
    11941 ggtatgataa gtaaagtgac ttatatcatg agtttacata acatatctat aagcaactta
    12001 agaatcttag aagtacgcga agatatatac ggtgctttaa aaattagttt caaaaatcct
    12061 actgaccgag aacgcggtat gcaagcattg agtgattttg attgttatat ccaataatta
    12121 ttaagtctct tttgataaat tcccatgtat tcttagatat tattgtgaaa tctattgtga
    12181 tatatgaaat atttatcttt agagacttat tttatacgaa tcacagttat atattcctta
    12241 aaccgtatag tcattacatt ttatgtccag ccattaaacc taatctacca tgttttgtac
    12301 ctgcttctgt aaacgatact gctttggaca caataccttt gccgtatttc aaatgtaatt
    12361 ggtctatcgt tttagctagg cattcgtcac gtttgcgttg gtattcatct tcaaacagac
    12421 ttaactgtcg ctcatcctca ttaataaatt gactcaaaga tatacttagc gtacgatata
    12481 gtgcttgttt attacataat ttatctgcaa aatgcattac tactttataa atatcttttt
    12541 ctaagtttgt tggatctttc aaagtatatt gcttatgtac accgccttca tcactatagc
    12601 caaaggcaaa atgtatcgtt cttgccactt tttttcttgc tcgaactctg ctagcaacat
    12661 cttcaattaa ctcttgcatt actacttttg cttcatcaaa atgataatct ctcattaata
    12721 tttgactttt gcatatcgat ggattgctga tcttgtgctt ttcacgtact ttactctgat
    12781 ctatcccatt cgcatgtaga tgcatatcaa cacctaaaat accgaactct ttttttaaaa
    12841 atttatatgg atatttcgct aaatctccta tagtaaaaat tcctctttta ttcaatttgg
    12901 cttctgttcg acgattaata ccccaaaaat ctcgcaaggg ctgaattggc cataatttcg
    12961 ttggtacatc ttgatatcgc cattcagcta taccattttg actatgcttc gcttcaacat
    13021 ccatagcaat tttacttaat aacatattag aaccaatgcc cacagtacaa taaatgcctg
    13081 tttcttcata aatttcacgt ttaagtcttt cgcaaaatgc atgtactgta gaactaaatc
    13141 tatgatagct atcagtaaca tccataaaaa attcgtcaat actatattgg tgtaaatctt
    13201 caggtggaat ataacgcaat gcaatcttag aaatagcaac tgaaacatta agatatttac
    13261 gcatacttgg attgataatg taaatatcat ttctatgtgg tatttcaaac aatcgcgacc
    13321 ctgtcttgat gcctaattct tttaatttag gtgtcgcagc caatattaca gaaccctgac
    13381 gcttagtatc tgcaacaaca gctaactttg tttctaatgg atctagcccc ttttcaatac
    13441 aagaaacact cgcaaaaaaa cttttttggt caatacatag aacatcccta tcttctaata
    13501 aatgataatt atacactgtt attcctccct tacttgaacc ttattatata cgaacacgtg
    13561 ttctatttca actattttag aacaaatgtt ctgttatttt gcaaatgaat ttttaagtat
    13621 tacatgatat actcaaatta ataatgatta ggaggtttgg atgatgccaa tcgtcaatgt
    13681 aaaattatta gaaggtcgtt cggatgaaca attaaaaaat ttagttagcg aagtaactga
    13741 cgccgtagaa aaaacaacgg gggcaaatag acaagcaatt cacgttgtta tagaagaaat
    13801 gaaaccaaac cattatggtg tggctggcgt aagaaagtca gatcaataat tcttcataag
    13861 atgcatgcca attaattctt tgaaaacgaa caaggcgact tctatctgag tatgatagaa
    13921 atcgccttgt ttatttttaa tcttcatcta aaaagtcttt aatagcttgt ttatttgtgt
    13981 ttttattaat ctgtaatgca ctaccatcag tatttgtatt gacatcttcg tatgagttct
    14041 tgattggcac agtcaatgac ttaacatctt tttcacctcg gatgccaaaa ctcaaacctg
    14101 tttggaaaat ccctgaatca ggaatgtttg tattcacata gcctcttaaa atacctgcaa
    14161 cttttggtaa tttaacaact gttctaaaat taaccatttc ttttttcaat gtttgcatca
    14221 cttgttgctg acgtcgcacg cgtccgaagt caccttcagg gtcgtgacgg aatcttgcat
    14281 aaccaagtaa ttctttacca ttcaacctat ggttaccctt tttcaaagat acaccaatat
    14341 ttttcgacat atccttttcg acattaattg gtacaccttc tggcattaat tcatcaatca
    14401 ttttctcaaa tccagtaaaa tcaactactg cataatattc aggattaatt cctaaatttt
    14461 tatcaagtgt ttttctaagt agctctggac cacctaaagc gtatgctgaa ttaattttgt
    14521 gttttccata tcctggaata tctgcataaa tatcacgcat gacagacatc attttcattt
    14581 ttttattgat aaagtcatat tgaacaacca tgatagaatc tgttcttgat tgtccacctt
    14641 gtgctttatc tgcaccgagt acaagaatag aaattttacc atcatttttt actggtccat
    14701 taaattgatg tactttaaca tctttcgcat gtttcttggc atattctaca ccgctattgt
    14761 aactatgtac aatatataca actaatgcga taagtaaaat tacaacaatc agaagaatga
    14821 taggtaattt cctaattttc ttcttctttt ttcgcttagg cgccgaagtg cgatgttcac
    14881 tttgacgtct atattcgttg tcattagttt ctttatccat atttacctac cttatatctt
    14941 caaaaataga tttttgtgta ctataataat cacatattaa cgttatataa aaacaattca
    15001 aaaagcaagt gatttcttaa taaatcgtac taaggtctaa tgaaaggatg tcaaaaatga
    15061 atattaatac agcttatttt gccggaggtt gcttttggtg tatgacgaaa ccatttgaca
    15121 cctttgacgg catagaaaaa gtaacttctg gatatatggg cggacatatt gaaaatccta
    15181 cttacgaaca agtaaaatca ggtacgagtg gtcatttaga aactgttgaa attcaatatg
    15241 atgttgcatt attctcatac aataagttat tagaaatatt tttctcagtc attgacccat
    15301 tagatacagg tggtcaatat caagaccgtg gtcctcaata tcaaacagct attttctaca
    15361 ctaatgatca tcaaaaagaa ctcgctgaga catatatcga gcagcttaaa aatacgatta
    15421 atgctgataa ggcaattgca acaaaaatat taccagcgtc acaattttac aaagccgaag
    15481 actatcacca agatttttat aagaaaaatc cagagcgcta tgcagaagaa caaaaaatac
    15541 gccaagaata caaaaataag caataacatt tatacttgat gaaactgatt gggctgataa
    15601 taaaagttag gcgacttaac ttaagctcat ttcaatatta aaatacggta cagataggaa
    15661 acttaaatat tcttatctgt accgtttttt ataaatcatt ctttatagaa accaaaaaat
    15721 ccaagcgctt caggcataac tgtatacctc ttagcacttg gattttaatt atttgtgacg
    15781 tattacacgc attactttag aaagtgattc ccaaagcgaa ttatctttac gataaactaa
    15841 aaagcgtggt tcccaattcg gattatattt agatttataa cgacgtaatc cttggaaacg
    15901 atatagaccg ttgaaatgtt caaagacacg gcctgcaagt cgttctctta aatatgaata
    15961 atgcaattga ccaacgttcg ataacgttgc catacccata ttaaattttg tataaccttg
    16021 ttctttactc caaagtaaca tgtgcaagta taatccatcc attaatggta aatctaactc
    16081 tggcaaccat ctaattaaat cgactgaaat ggcatcatta aagtatgttg gcattaaact
    16141 acagaatgca attacttcat tttcttcatt tcgcattaca ccaattggcg ctttagataa
    16201 gtattcttca ttaaattgac ccacagagaa atgcatttcc tgacgattat ctagccataa
    16261 atcacttaca tgttgaagct catttataaa ttcagttgaa aacggtggtt caataatttc
    16321 gaatgaaata ttaagttcat cgaatttgtt taaagtcgct ctaaatccac ggcgtttttt
    16381 acctgaagtt gaaaattgcg ttaaatcaat aattgcttct tcacctaatt tgaaaaattg
    16441 gttaccgaaa ttatgatata aaggcatgtg ttgatctgta acttgataga atataacatc
    16501 atagcctaaa tactcagcgt aattatagaa tgcttctaac aattcatcaa aggcattttc
    16561 atcacctaac ggatctccaa gaaccactaa tgaacttgct ttataacgat acattaaaaa
    16621 tgctgtttta ttttcattag tgaaaaactg cttgtcacca ctatatatca agtgactcaa
    16681 ataattaccg ccgtactgat taataatctc ctcgcaatct tcaattttag aagaaatacg
    16741 tactttgcta aattgataat caaacaacca tgcaatcata cctatgatga tagcgatgat
    16801 taaaatcgta agccagaagt aatagcgcaa tacagatgta tgcatttcaa tcgtataaat
    16861 atctaatgca tataacgttc cagcaataaa tatatggtta acatataaaa taaataaact
    16921 gaataaaagc attgcaacta tatttctcat tcttactggg cgtttcaacc tacgtgctct
    16981 acggaaagct acaataagca gaacaaaaat aatagctaac catgttatta aaatatatga
    17041 agcgtaagtg aagaatgtcg ccactgtgat taataaaatt gaaatcatag caaagataat
    17101 ggcacgtcta ctttgcttat aaataccaac tacattcagt aaaagtaata aacaagcact
    17161 agtatgaatt gccaatagaa tataatacgt taagtgattt ccatcatata aagcatcgta
    17221 aacaatcgtt aagttattta caaaaaagat catacttgta aagaatacta aaattgctaa
    17281 tgataatgat ggaattttag caataatatc cttttgataa gacattaaaa atgacgtaac
    17341 atctttagca ggaataaagt atttagatcc ctcaatgtac ttcttagctg atgtaccaaa
    17401 ttcaaatgat gataaaatta atgcaataat taccggtaca aaatagtacg caaaacgata
    17461 tagaagtagc attaataata ctttttcctc agggacacct aaagttttaa atcctagtaa
    17521 tacaactaaa tcgaaagcgc cgaaaccacc aggaataaag ctgactaaac ctgataatgc
    17581 agcaatgata aatattgcaa taaaggacat gaatgataca tgagcgtcaa caattacacc
    17641 acagaaatat aatacaactg cagctgctaa ccattcaaca cacgacacta aagtgcagta
    17701 caatcctaca aaacgattgt ttttatcggg tggtctaacc attgaataaa taatgaataa
    17761 tggtaagaaa aatgacacta catataatac ccatcttacc catgtaatct tatctaagat
    17821 taaagatgca tcgaaaacat ggaatacaat tagcaatgat aataagctta aacctgtcaa
    17881 cattgaaata agtattaagg atataaagtg aactaatttc tttttatcat gcgtatagtt
    17941 tttataaacc attgctctaa cgcctgcacc aataaagcca ccgaaaccta caatcgcatt
    18001 caatgcattg atgatataac ttactcttaa aactttgcct aaggatatat ccatttttaa
    18061 agctctagat aaaatcacat catacattga tagaataaca agcgatgccc caccaccaat
    18121 aaatagtaac actaaggaca tacggttaat cttactaaat tcaaccaacg tatctttaaa
    18181 gttaatacca gataactccc gatacaatgt gattgctaca aaaataaata aagctgtagc
    18241 aaacgtaatt tttaagattg aaaatatttt gtttttaact tcctgattca ttttttcaca
    18301 tcaattctaa ttatttctgt tataaatcaa aatatatcat ata
//
LOCUS       DANBWH010000034        18036 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.035, whole genome shotgun sequence.
ACCESSION   DANBWH010000034 DANBWH010000000
VERSION     DANBWH010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18036
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.035"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            341..655
                     /locus_tag="PPY92_000799"
     CDS             341..655
                     /locus_tag="PPY92_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDJ2624818.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            671..1057
                     /locus_tag="PPY92_000800"
     CDS             671..1057
                     /locus_tag="PPY92_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDJ2624819.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            1086..2471
                     /locus_tag="PPY92_000801"
     CDS             1086..2471
                     /locus_tag="PPY92_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDJ2624820.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            2474..2626
                     /locus_tag="PPY92_000802"
     CDS             2474..2626
                     /locus_tag="PPY92_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDJ2624821.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            2649..3854
                     /locus_tag="PPY92_000803"
     CDS             2649..3854
                     /locus_tag="PPY92_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDJ2624822.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            3897..4118
                     /locus_tag="PPY92_000804"
     CDS             3897..4118
                     /locus_tag="PPY92_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624823.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            4235..4732
                     /locus_tag="PPY92_000805"
     CDS             4235..4732
                     /locus_tag="PPY92_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDJ2624824.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            4707..5213
                     /locus_tag="PPY92_000806"
     CDS             4707..5213
                     /locus_tag="PPY92_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDJ2624825.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            5197..7644
                     /locus_tag="PPY92_000807"
     CDS             5197..7644
                     /locus_tag="PPY92_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDJ2624826.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            7647..9824
                     /locus_tag="PPY92_000808"
     CDS             7647..9824
                     /locus_tag="PPY92_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDJ2624827.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGADRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRNNVNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            9821..10822
                     /locus_tag="PPY92_000809"
     CDS             9821..10822
                     /locus_tag="PPY92_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDJ2624828.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            10819..11751
                     /locus_tag="PPY92_000810"
     CDS             10819..11751
                     /locus_tag="PPY92_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDJ2624829.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            11996..12112
                     /locus_tag="PPY92_000811"
     CDS             11996..12112
                     /locus_tag="PPY92_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDJ2624830.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            12128..14047
                     /gene="tet(M)"
                     /locus_tag="PPY92_000812"
     CDS             12128..14047
                     /gene="tet(M)"
                     /locus_tag="PPY92_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HDJ2624831.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            14167..14334
                     /locus_tag="PPY92_000813"
     CDS             14167..14334
                     /locus_tag="PPY92_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDJ2624832.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(14394..14747)
                     /locus_tag="PPY92_000814"
     CDS             complement(14394..14747)
                     /locus_tag="PPY92_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDJ2624833.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            15252..15674
                     /locus_tag="PPY92_000815"
     CDS             15252..15674
                     /locus_tag="PPY92_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDJ2624834.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            15671..15901
                     /locus_tag="PPY92_000816"
     CDS             15671..15901
                     /locus_tag="PPY92_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDJ2624835.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            16362..16565
                     /locus_tag="PPY92_000817"
     CDS             16362..16565
                     /locus_tag="PPY92_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDJ2624836.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            16647..17864
                     /locus_tag="PPY92_000818"
     CDS             16647..17864
                     /locus_tag="PPY92_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDJ2624837.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
BASE COUNT         5918 a         3225 c         3752 g         5141 t
ORIGIN      
        1 tattaaaaat agcataaaaa tctagttatc cgcataaaaa ctggacttat cacactttat
       61 caaggtcaaa accactcaat ttactactaa tttactactt atgaatgagc tttgatacga
      121 cgatttatcc ttgaaaagtg aagatataaa gatacttcca ataaaatttg aatatttaat
      181 aggtagacac ttcaaaaaat gaggtgtcta tttttttacc cgattttgaa aggaagtgaa
      241 cttatgaaaa caaaaaatca agaatcaaaa ggtcgttccc cactctttaa gaccatcaaa
      301 cattcattca gccaataaaa aagaaaggat aggtaaaaat atggaactta aatttgtgat
      361 tcccaacatg gaaaaaacat tcggcaattt agaatttgct ggcgaggata aagtcgttca
      421 gcgaagaatc aacggacggc taactgtctt atcaagaagc tataatctct attctgatgt
      481 tcaaagagca gatgatattg tggtggtgct tcctgctgaa gctggcgaaa aacatttcgg
      541 ctttgaggaa cgtgtgaagt tagtcaatcc acgtattacc gcagagggct acaaaatcgg
      601 cactcgtggt tttacaaatt accttttaca tgctgacgac atgataaaag aataaagaaa
      661 gagaggaaaa atgatgagat tagcaaatgg cattgtatta gataaagaca cgacttttgg
      721 agaattgaaa ttctctgctc tacgtcgtga agtgagaatc caaaatgaag acgggtcggt
      781 ttcagatgaa atcaaggaac gtacctatga cttaaaatcc aaaggacaag gacgcatgat
      841 tcaagtaagt attcctgcca gcgtgccttt gaaagagttt gattataacg cacgggtgga
      901 acttatcaat cccattgcgg acaccgttgc tactgccacc tatcaaggag cagatgttga
      961 ctggtatatc aaggcagacg atattgtgct gacaaaggat tctagttcat tcaaagctca
     1021 accacaagca aagaaagaac cgacacaaga caaatagtcg ctaggtagaa aggagacttt
     1081 ttcgcatgaa acagcgtggt aaaaggattc gcccatctgg taaagattta gtctttcatt
     1141 ttacgatagc gtcactcctg cctgttttcc tgctggttgt cggactgttt catgtgaaga
     1201 caatccagca gatcaactgg caggatttta acctatcaca agcagataag attgacattc
     1261 cctatttaat tatcagtttc agtgtcgcaa ttcttatctg cttgctggta gcgtttgtat
     1321 tcaaacgggt tcgctatgat acggttaaac aactttacca ccgtcaaaaa ctggcaaaga
     1381 tgatacttga aaacaagtgg tatgaatctg aacaggtcaa aacagagggt ttctttaaag
     1441 atagtgctgg tcgtacaaag gaaaagataa cctacttccc taaaatgtat tatcgactta
     1501 aaaatggctt gatacagata cgggtggaaa tcacgctggg aaaatatcaa gaccaactct
     1561 tacacttgga aaagaaatta gagagtggct tgtactgtga gctgacggat aaagagttaa
     1621 aggattccta tgtggaatat actttgctct atgacaccat agccagtcgt atttctattg
     1681 atgaagtaga agctaaagat ggtaaacttc gcttaatgaa aaacgtatgg tgggaatatg
     1741 ataagctccc tcatatgttg attgctggtg gtacaggtgg cggtaaaact tactttatac
     1801 tgacactgat tgaagccttg cttcatacag attcaaaact gtatattctt gacccgaaaa
     1861 atgctgatct tgcggactta ggttctgtga tggcaaatgt ctactataga aaagaagact
     1921 tgctttcttg cattgaaaca ttctatgaag aaatgatgaa acgtagtgag gaaatgaagc
     1981 agatgaagaa ctataagact ggcaaaaatt atgcttactt aggtctcccg gcacacttct
     2041 taatctttga tgaatacgtc gctttcatgg aaatgctggg aacaaaagaa aacaccgcag
     2101 ttatgaataa gctgaaacag attgtcatgt taggtcgtca agctggcttc tttctaatac
     2161 tggcttgtca acgtccagac gcaaaatatt taggcgacgg aatccgtgat cagtttaatt
     2221 tcagagtggc tttaggtcgt atgtctgaaa tgggctatgg catgatgttt ggcagtgacg
     2281 tacaaaagga tttcttctta aagcgaatca aaggtcgtgg ctatgttgat gtaggaacaa
     2341 gtgtcatatc agagttttat actccccttg taccaaaagg atatgatttc ttggaggaaa
     2401 ttaaaaagtt atccaacagc agacagtcca cgcaggcgac gtgcgaagcg gaagtcgcag
     2461 gtgtggactg atcttgctgg ctggtgtggc aatagccacg ccagcactta accccccgta
     2521 tctaacaggg gggtacaaat cgacaggaaa cagtcaaaaa aacattagaa aatcctttgg
     2581 ttacaaggga tttacaaaat ttcagcgtat gtcaaatggg ctttaaaagt tgacatacgc
     2641 ctttttgatt ggagggattt ttactgaatg aacaaacttg gttacagcat ttaaaagaaa
     2701 aacgcttggc ttatggacta tctcaaaacc gtttagctgt tgcgactggt attacaaggc
     2761 agtatctaag cgatattgaa acaggaaaag tcaagccatc agaggattta cagcagtccc
     2821 tttgggaagc tctggaacgc ttcaatcccg acgctcccct tgaaatgctg tttgattatg
     2881 taagaattcg ctttccgaca acagacgtac agcaggtggt cgaaaacatc ttacaactga
     2941 aactgtccta ttttcttcat gaggactatg gtttctattc ttattcagag cattatgctt
     3001 taggcgacat attcgtcctt tgctcccatg aactggacaa aggagttctg gtggaattga
     3061 aaggtcgtgg gtgcagacaa tttgaaagct atcttctggc acaacaaaga agctggtatg
     3121 agttctttat ggacgttttg gtggctggcg gtgtgatgaa acgccttgac cttgccatta
     3181 acgataagac agggatttta aatatccctg tactcactga aaagtgccaa caggaagaat
     3241 gtatctccgt cttccgcagt tttaaaagct atcgcagtgg cgaactggta cgcaaagagg
     3301 aaaaggaatg tatgggaaac accctctata tcggttcatt acaaagtgaa gtttatttct
     3361 gtatctatga aaaggactac gagcagtaca agaaaaatga tattcccatt gaagacgcag
     3421 aagtaaaaaa ccgttttgag attcgattga aaaatgagcg tgcctattat gcagtccgtg
     3481 atttactcgt ctatgacaat ccagagcata ccgcctttaa aattatcaat cggtatatcc
     3541 gttttgtaga taaagacgat tccaaacctc gttctgattg gaaactgaat gaagaatggg
     3601 cttggtttat tgggaacaat cgtgaacgat taaaactaac cacaaaacca gagccttact
     3661 ccttccaaag gacgctgaac tggctatctc atcaagttgc cccgacctta aaggttgcga
     3721 ttaaacttga tgaaatcaac cagacgcagg ttgtaaaaga cattctcgac catgcgaaac
     3781 tgacagaccg acacaagcag attttgaagc aacagtcagt aaaagaacag gacgtgataa
     3841 caacaaaaaa ataactcaaa tacaaattca ttgaatatag agaggagaac atttttatga
     3901 attttggaca aaacctttat aactggtttc tatcaaacgc tcaatcactg gtgcttttag
     3961 caatcgttgt gattggcttg tatcttggct tcaagcgtga gtttagcaaa ctgattggct
     4021 ttttaattat tgcgattatt gcggttggct tagtcttcaa cgctgctgga gtaaaagaca
     4081 ttttactaga gctattcaat cgcattattg gtgcttaaat aaaaccgttc ttttgtggaa
     4141 tataagtggt tttcttatgt tccgcaaagg aatggtacac caaacgaagt gcggtaggga
     4201 tttttgaatc tctacaaaga aaggacgtga atatatggac gatatgcaag tctatattgc
     4261 gaatttaggc aaatacaatg agggcgaatt ggtcggtgcg tggtttacct ttcccattga
     4321 ctttgaggaa gtcaaagaga aaatcggctt gaatgatgaa tatgaggaat acgccattca
     4381 tgactacgag ttacccttta cggttgacga atacacttcc attggcgaac tcaatcgact
     4441 atgggaaatg gtatcggaat tacccgaaga attacaatcg gagctatctg ctctgctcac
     4501 tcatttttca agcattgaag aactaagcga acatcaagag gatattatca ttcattccga
     4561 ttgtgatgat atgtatgacg tggcacgcta ctacattgaa gaaacgggtg ctttaggcga
     4621 agtaccagct agtcttcaaa actatattga ttatcaagcc tatggtcggg atttagacct
     4681 ttcaggaacg tttatctcaa ccaatcatgg gatttttgaa atcgtctatt aaatctgtcg
     4741 gtacattact actggcagat tttctatttt acggggtggc tcaatcagct acccctattt
     4801 tttatgaaag gattgattac atgaagaaaa tacgaagcta taccagtatc tggtctgtgg
     4861 aaaaggtact gtattctatc aatgatttta gacttccgtt tcccataacc tttacgcaaa
     4921 tgacatggtt tgtcgtgtca ctctttgcag tgatgatact tggcaacttg ccccctcttt
     4981 ccatgataga gggagcattt ctcaaatact ttgggattcc tgtggctttc acatggttta
     5041 tgtctacaaa aacttttgat ggtaaaaagc cttatggatt tttgaagtct gtcattgctt
     5101 atgcactgcg accaaagctg acctatgcag gaaaaaaagt aacgcttggc agaaaccagc
     5161 cacaagaagc cattacagca gttaggagtg aattttatgg catatccaat taaatacatt
     5221 gaaaacaatc tcgtctggaa taaagacggg gaatgttatg cttactatga gcttgttcct
     5281 tacaattact catttctaag tccagaacag aaaatacaag tgcatgattc tttcagacag
     5341 cttatcgcac aaaatcgtga tggcaaaatt catgctttac aaatcagtac agaatccagc
     5401 atacgttctg cacaagagcg ttccaaaaat gaagtcactg gcaagctcaa agcggttgcc
     5461 tatgacaaaa tcgaccaaca gacagacgct ttaatatcca tgattggcga aaatcaagtg
     5521 aactaccgtt tctttatcgg ctttaagttg cttctcaacg atcaggagtt ttctatgaaa
     5581 agtcttaccg ttgaagcaaa aaatgctttg tctgattttg tctatgatgt gaaccataag
     5641 ctgatgggcg attttgttag tatgagtaat gatgaaatcc tgcgttttca gaagatggaa
     5701 aagctcttag aaaataaaat ctctcgtcgt ttcaaaatcc gcaggttaga taaggacgac
     5761 ttcggctatc tgattgaaca cctttacgga cagacaggca ctgcctatga agagtatgag
     5821 taccatctat caaagaaaaa gctggataat gaaacgctga ttaaatacta tgacttgatt
     5881 aagcctactc gctgtttggt ggaagaaaaa cagcgatatt tgaaaatcca gcaggaagat
     5941 gaaaccgtct atgtagctta ctttaccatt aacagcattg tcggagaact ggacttcccg
     6001 tcctctgaaa tcttctacta ccagcaacag caatttacat tcccgattga tacgtcaatg
     6061 aatgtggaaa ttgtagcgaa tcgtaaagcc ctatctactg tccgcaataa aaagaaagaa
     6121 ctgaaagact tggataacca cgcttggcaa agtgataatg aaaccagctc caatgtggcg
     6181 gaagctctgg aaagtgtgaa tgagctggaa accaatttag accaaagcaa ggaatctatg
     6241 tacaagctgt cttatgtggt aagggtatca gcaaatgatc ttgacgaact caaacgtcgt
     6301 tgtaatgaag tgaaagattt ttatgacgat ttaagcgtaa aactggtacg accatttggg
     6361 gatatgctcg gcttacatga agaattttta cctgccagca agcgttatat gaatgattat
     6421 attcaatacg tgacctctga tttcctcgct ggtttaggtt ttggtgctac tcaaatgctg
     6481 ggggaaaatg aggggattta tgttggctac agcttagata ctggacgcaa tgtctatctg
     6541 aaacctgctc ttgccagtca aggggttaag ggttcagtaa ccaatgcgtt agcgtcggct
     6601 tttgttggtt cgctgggtgg tggtaaatcc tttgcgaata accttatcgt ctattatgcg
     6661 gtgctttatg gggcacaagc agtgattgta gacccaaaag cagaacgtgg cagatggaaa
     6721 gaaaccttgc cagagatttc ccatgaaatc aatatcgtca ctctgacttc tgatgagaaa
     6781 aacaaaggct tacttgaccc ttatgtgatt atgaaaaatc ccaaagattc tgaatcactg
     6841 gctattgata ttctgacatt ccttacgggg atttcctctc gtgatgggga acgcttccca
     6901 atccttagaa aagccattcg tgcagtaacc aatagtgaag tacgagggtt gatgaaagtg
     6961 attgaggaat tacgggttga gaatacgcca ctaagtacca gtatagccga ccatatcgaa
     7021 agttttacag actatgactt tgcacattta ttattcagta atggttatgt ggagcagtct
     7081 atcagcttag aaaaacaact gaacattata caggttgcgg acttggtact tcccgacaag
     7141 gaaacttcct ttgaggaata taccactatg gagcttttat ccgttgctat gctgattgtc
     7201 attagtacct ttgctttaga ctttatccat acagaccgaa gcattttcaa gattgtagat
     7261 ttagacgaag catggagctt tttacaggta gcacaaggaa aaacactatc tatgaagctg
     7321 gttcgggctg gtcgtgctat gaacgctggg gtatatttcg tgacccaaaa tacagacgac
     7381 ctcttagatg aaaaactgaa aaataacctc ggcttaaaat ttgcatttcg ttccactgac
     7441 cttaacgaga ttaaaaagac cttagccttt tttggtgtag acccagagga cgaaaacaat
     7501 cagaagcgat tgcgtgattt ggaaaacggg caatgcctta tcagtgattt atatggtcgt
     7561 gtcggtgtga tacagttcca ccctgtattt gaagaactgc tccatgcctt tgataccaga
     7621 ccacctgtgc gaaaagaggt gtaaatgtga aaccatcaat agtaaacaga ataaaatcaa
     7681 actggacgct gaaacgtcta ggtaaagtgg caatgacagt ggctttcaca cttgtgattg
     7741 ccatttttct tttagccatg ctgggaacgg tggttcaagc tgcgggcttg gtagatgata
     7801 cggtcaatgt ggcaaatgaa tacagccgat acccacttga aaactatcaa ctggattttt
     7861 atgtggataa tagctggggc tggcttccgt ggaactggtc ggacgggatt ggaaaacagg
     7921 tcatgtatgg actatatgcc attaccaatt ttatttggac aatcagtttg tatgtttcca
     7981 atgcgacagg ttacttagta caggaagcct attccttaga cttcatttcc gctacagcag
     8041 attccattgg taagaatatg cagaccttag ctggtgtgag tgcaaacgga ttttcaacag
     8101 agggtttcta tgttggattc ctcttactct tgattttggt tcttggggtt tatgttgcct
     8161 atacgggact gataaagaga gaaaccacaa aggcaattca tgccattatg aattttgtgc
     8221 tggtgtttat cctatcggct tcctttattg cctacgctcc cgactacatt aaaaaaatca
     8281 atgacttttc atcagacatc agtaatgcca gtttatcact tggcacgaag attgtcatgc
     8341 cccattccga tagtcaaggc aaggacagcg tggacttaat cagagatagc ctgttttcca
     8401 tacaggttca gcaaccgtgg ctactgcttc aatacaacag ttcagacatt gaaagtattg
     8461 gtgctgaccg tgtggaaagt ctactttcaa ccagtccaga cagtaataat ggagaagaca
     8521 gagaaaaaat tgtagcagaa gaaatcgaag acagaaacaa tgtgaacatg acgattacaa
     8581 agaccattaa ccgtttaggt acagtcttct tcctatttgt cttcaatatt gggatttcca
     8641 tatttgtatt cctattaaca ggaatcatga ttttctcgca ggtacttttt atcatctatg
     8701 ctatgtttct gcctgtgagc tttattttaa gcatgattcc atcatttgat ggtatgtcaa
     8761 aacgagccat aacaaagctc tttaatacca ttttgacacg agctggaatc acattgatta
     8821 ttacgacagc atttagtatt tcaaccatgc tctatacctt atcggctggt tatccgttct
     8881 ttttgattgc ttttctacag attgtgacct ttgcaggaat ctacttcaag ctgggcgatt
     8941 taatgagtat gttttctcta cagagtaacg attctcaaag tgtgggaagt cgtgtgatga
     9001 gaaaacctcg tatgcttatg cacgctcaca tgcaccgtct acagcggaaa cttggacgtt
     9061 ccatgactac tctaggggct gggtctgcca ttgttacagg taaaaaagga cagtcgggtt
     9121 cggggagttc tgcaaggaca caagcagatc actcccgacc agacggaaag gaaaaatcaa
     9181 cacttggaaa acgtatcggt caaaccatcg gtacagtagc tgataccaaa gacagaatgg
     9241 tagacactgc tagtggtttg aaagaacagg ttaaagattt gccgaccaat gcaagatatg
     9301 cagtatatca aggaaaatcc aaagtaaaag agaatgtccg tgatttaacc agtagtattt
     9361 ctcaaaccaa agcggacaga gccagtggac gcaaggaaca gcaggaacaa aggcgaaaaa
     9421 ccattgcgaa gcgtcgctct gaaatggaac aggtcaaaca gaaaaaacag cctgcttctt
     9481 ctgttcatga aagaccgact acaagacaag aacaatatca tgatgaacag acctcaaaac
     9541 agtctaatat tcagacttca tataaggaat ctcaacaagc caaacaagag cgtccagcag
     9601 ttaagtccga tttttcaagt ccaaaagtgg aacgccaagg caataccgtt caagaaaaaa
     9661 ccgttcaaaa gccagcaact tcaaccacta cagcagatag aacttcacaa cgtccaatca
     9721 caaaagaacg tccgtctact gttcaaagag taccactaca aaatacaaga agtagaccac
     9781 caatcaaaac cgccaccatt aagaaagtcg gtaagaaacc atgaagttga aaactttagt
     9841 gattggtggt tctggattat tcttgatggt cttctcactg cttctgtttg ttgccatttt
     9901 attttcagat gaacaggaca gcggaatttc caatattcat tatggaggtg tgaatgtttc
     9961 cgcagaagtg ctggctcata agcctatggt agaaaaatat gccaaagaat atggcgttga
    10021 agaatatgtc aacatacttc ttgcgattat acaggtggaa tcgggcggta ctgcggaaga
    10081 tgttatgcag tcctcggaat ccctcggtct tccacctaat tcattgagta cagaagaatc
    10141 cattaagcaa ggtgtgaagt atttcagtga attattagcc agtagcgaaa ggctcagtgt
    10201 agatttagaa tcggttatcc agtcctacaa ttatggtggt ggtttcttag ggtatgtggc
    10261 taatcgtgga aataaatata cctttgaact ggctcaaagt ttctcaaaag agtattcagg
    10321 tggcgaaaaa gtgtcttacc ccaatcccat agccatacct atcaatgggg gctggcgata
    10381 caactatggc aatatgtttt atgtgcaact ggtaacgcag tatcttgtca caacagagtt
    10441 tgatgatgat acggtacaag ccatcatgga cgaagcactg aaatatgagg gctggcgata
    10501 cgtttacggt ggagcttccc cgactacttc ttttgattgt agcggactga cacaatggac
    10561 gtatggaaaa gctggaatta acttaccacg aaccgcacaa cagcaatatg atgtgaccca
    10621 gcatatccca ctatcggaag cacaagctgg cgatttggtt ttctttcatt ctacctataa
    10681 cgctggctct tatattactc atgttgggat ataccttggc aataaccgta tgtttcatgc
    10741 aggcgaccca atcggttatg ccgacttaac aagcccctac tggcaacagc atttagtggg
    10801 agcaggacga atcaaacaat gagaaaggaa gatttaatga tgaaatttag aaaaaatcag
    10861 aataaagaaa aacagatacc aaaggaaaag aaacctcgtg tctataaggt caatcctcat
    10921 aaaaaggttg tgattgcctt gtgggtactt ttagggctta gtttcagctt tgcgatattc
    10981 aagcacttta cagctataga tactcatact attcacgaaa caactatcat agaaaaggaa
    11041 tacgttgata ctcatcatgt agaaaatttt gtagagaact ttgcgaaagt ctactattca
    11101 tgggagcaat ccgataagtc cattgataat cgaatggaaa gtctaaaagg ctatctgaca
    11161 gatgaacttc aagctctcaa tgttgataca gtacgcaaag atattcctgt atcgtcttct
    11221 gtaagaggat ttcagatatg gacggtagag ccaactggcg acaatgagtt taatgtaacc
    11281 tacagtgtag accagctcat tacagaggga gaaaatacaa agaccgtcca ctctgcttat
    11341 atagtgagtg tctatgtaga tggttctgga aatatggtac tggttaagaa tccgaccatt
    11401 accaacatac ctaagaaatc aagttataaa ccaaaagcca ttgaaagtga ggggacggtt
    11461 gattccatta caaccaatga aatcaatgag tttttaacga cgttcttcaa gctctatcct
    11521 acagcgacag ccagtgaact ttcctactat gtgaatgacg ggatattaaa accaatcgga
    11581 aaagagtaca tctttcaaga actggtaaat cctattcaca atcgtaagga taatcaagtc
    11641 acggtatcgc tgacagtgga gtatatcgac cagcagacca aagcaacgca ggtatctcaa
    11701 tttgatttgg tacttgaaaa gaacgggagt aattggaaga ttatagaata acaaatattg
    11761 gtacattatt acagctattt tgtaatcacg tactctcttt gataaaaaat tggagattcc
    11821 tttacaaata tgctcttacg tgctattatt taagtatcta tttaaaagga gttaataaat
    11881 atgcggcaag gtattcttaa ataaactgtc aatttgatag tgggaacaaa taattggatg
    11941 tcctttttta ggagggctta gttttttgta cccagtttaa gaataccttt atcatgtgat
    12001 tctaaagtat ccggagaata tctgtatgct ttgtatgcct atggttatgc ataaaaatcc
    12061 cagtgataaa agtatttatc actgggattt ttatgccctt ttgggttttt gaatggagga
    12121 aaatcacatg aaaattatta atattggagt tttagctcat gttgatgcag gaaaaactac
    12181 cttaacagaa agcttattat ataacagtgg agcgattaca gaattaggaa gcgtggacaa
    12241 aggtacaacg aggacggata atacgctttt agaacgtcag agaggaatta caattcagac
    12301 aggaataacc tcttttcagt gggaaaatac gaaggtgaac atcatagaca cgccaggaca
    12361 tatggatttc ttagcagaag tatatcgttc attatcagtt ttagatgggg caattctact
    12421 gatttctgca aaagatggcg tacaagcaca aactcgtata ttatttcatg cacttaggaa
    12481 aatggggatt cccacaatct tttttatcaa taagattgac caaaatggaa ttgatttatc
    12541 aacggtttat caggatatta aagagaaact ttctgccgaa attgtaatca aacagaaggt
    12601 agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa tctgaacaat gggatacggt
    12661 aatagaggga aacgatgacc ttttagagaa atatatgtcc ggtaaatcat tagaagcatt
    12721 ggaactcgaa caagaggaaa gcataagatt tcagaattgt tctctgttcc ctctttatca
    12781 tggaagtgca aaaagtaata tagggattga taaccttata gaagttatta ctaataaatt
    12841 ttattcatca acacatcgag gtccgtctga actttgcgga aatgttttca aaattgaata
    12901 tacaaaaaaa agacaacgtc ttgcatatat acgcctttat agtggagtac tacatttacg
    12961 agattcggtt agagtatcag aaaaagaaaa aataaaagtt acagaaatgt atacttcaat
    13021 aaatggtgaa ttatgtaaga ttgatagagc ttattctgga gaaattgtta ttttgcaaaa
    13081 tgagtttttg aagttaaata gtgttcttgg agatacaaaa ctattgccac agagaaaaaa
    13141 gattgaaaat ccgcaccctc tactacaaac aactgttgaa ccgagtaaac ctgaacagag
    13201 agaaatgttg cttgatgccc ttttggaaat ctcagatagt gatccgcttc tacgatatta
    13261 cgtggattct acgacacatg aaattatact ttctttctta gggaaagtac aaatggaagt
    13321 gattagtgca ctgttgcaag aaaagtatca tgtggagata gaactaaaag agcctacagt
    13381 catttatatg gagagaccgt taaaaaatgc agaatatacc attcacatcg aagtgccgcc
    13441 aaatcctttc tgggcttcca ttggtttatc tgtatcaccg cttccgttgg gaagtggaat
    13501 gcagtatgag agctcggttt ctcttggata cttaaatcaa tcgtttcaaa atgcagttat
    13561 ggaggggata cgctatggct gtgaacaagg attgtatggt tggaatgtga cggactgtaa
    13621 aatctgtttt aagtatggct tatactatag ccctgttagt accccagcag attttcggat
    13681 gcttgctcct attgtattgg aacaagtctt aaaaaaagct ggaacagaat tgttagagcc
    13741 atatcttagt tttaaaattt atgcgccaca ggaatatctt tcacgagcat acaacgatgc
    13801 tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa aataatgagg tcattcttag
    13861 tggagaaatc cctgctcggt gtattcaaga atatcgtagt gatttaactt tctttacaaa
    13921 tggacgtagt gtttgtttaa cagagttaaa agggtaccat gttactaccg gtgaacctgt
    13981 ttgccagccc cgtcgtccaa atagtcggat agataaagta cgatatatgt tcaataaaat
    14041 aacttagtgt attttatgtt gttatataaa tatggttttc ttgttaaata agatgaaata
    14101 ttttttaata aagatttgaa ttaaagtgta aaggaggaga tagttattat aaactacaag
    14161 tggatattgt gtcctgtatg tggaaataaa acacgattaa agataaggga agatactgaa
    14221 ttaaaaaaat tccccctcta ttgtccgaaa tgcagacaag aaaatttaat tgaaataaag
    14281 cagttcaaag taactgtgat tacagagcca gacgcaaaga cgcagagccg ataaaatgag
    14341 attaatacaa tctcatttta tcggctcttt ccgttatgta tggattcttt taattagtct
    14401 tcgatgtttc ttgcttcgtt gataccgctg gctaaagatt ccattaagga tagttctttg
    14461 tctgtaaagc tatccatgta tttctctatc tgtaatcgtc gggtgctttt taccaagtta
    14521 ttagcaggta agaaaaattc atcaacggaa acatgaagta acgatacaag gtcataaaga
    14581 acttgtatgc tggggtgttg ccctttattt tcaatattag ttaagtaccg tgggtcaatt
    14641 tcaatcaatg ctcccacttg ttcacgagtt aaacctcgtt tcaatcgagc ttctttaatg
    14701 gctaaaccaa aggctctaaa atcatattta tcttcttttt tacgcatagt agaccacctc
    14761 tatacatttt attgttccta ctgaattaaa aacaggtata gaaaaacgtg ttatatggtt
    14821 tataggttta tatttaataa aaagcactac taaacgccaa taaaaaaaac cgttatatgg
    14881 tagtgctatt tacgctgtta aaatattgta tattacttcc aaatggcggt ttgttggagg
    14941 tcaacgtcgc catgaagtac atcatataca ataaatttcc ttacattggg ttcttgtcaa
    15001 aaaaagtcgt ctatctgcaa tagataagta cgtccaccaa tgtggtttta taaatcatat
    15061 agatagaata acagaagcat gtaaacagag aaataaatct gtttatatgc ttttttggct
    15121 attcagaact tttttacaaa gtttatttat cagtaatgca acaaatcccc ctttcacatt
    15181 gggactaaga gtgaaaggag ataaacgagc aaggctcact tcctttccta gacagaaagg
    15241 gggtgagaaa catgaaacca tcttcttttc agaccacaat agaaaatcag tttgactata
    15301 tctgtaaacg tgctatggaa gacgagcgaa agaattatat gctttatctt tcaaggattg
    15361 caaagcgtga ggtgtccttt tcggatgttg gcgattatct tgttagccag tttgcgacaa
    15421 cagataacta ttcaactgac tttcagattt ttacactcaa tgggttatca gtaggcgttg
    15481 aaaatgattt gttgagtgaa gcattacgtg agttgccaga caagaaacgt gaaattctac
    15541 tgctgtttta ctttatggac atgagcgatt cagaaattgc agacctgttg aaattgaacc
    15601 gttctactgt ctatcggcat agaaccagtg gactagcctt aattaaaaag tttatggagg
    15661 aatttgaaga atgaaaacac aatatcctat gattcccttt cctctcattg taaaggcaac
    15721 agatggcgat accgaagcga ttaaccagat tctacatcat tacagagggt acataacgaa
    15781 gcgttcccta cgacttatga aagatgaata tggcaatcaa agtatggtcg ttgatgaagt
    15841 cttacgtgga agaatggaaa ccagactgat tacaaagatt ttgtcatttg aaattaagta
    15901 atatcctctc tcctttcgtg gaagcgtgct aaaccattcc acgcttcccg aacagggagg
    15961 tttgttattc caccaaagca tattgagctt tcaatgtgtt ttgataggct aacgagccat
    16021 tgttctttga aaactgaata aaagtaatcg aatacgtttc gataagaaaa gagccaacgg
    16081 aactaaccgc catgacctat cttataaaga tagcgagcga ttcatgttag tgatccgaga
    16141 agcaatcttt agcaggattg cctgcaacga cattcttatc gtgataatga tactcccata
    16201 cagtcaatag tccgagcgtg ataaaaccgt cgcaggcaat gagtatggct acatgagaac
    16261 catgcagggg tggaactccc gtgagctttg ctaaagctgt tcgattgctg gtaaaacaac
    16321 ttttatgaaa tccaaataag tgatttggaa aggaggattt tatgaagcag actgacattc
    16381 ctatttggga acgttatacc ctaaccattg aagaagcgtc aaaatatttt cgtattggcg
    16441 aaaacaagct acgacgcttg gcagaggaaa ataaaaatgc aaattggctg attatgaatg
    16501 gcaatcgtat tcagattaaa cgaaaacaat ttgaaaaaat tatagataca ttggacgcaa
    16561 tctagcgtcg ccaaagggtc ttgtatatga taaaatagta ttaagtcgta tcaaggctct
    16621 ttccataaag gaaaggagca aatgccatgt cagaaaaaag acgtgacaat aaaggtcgaa
    16681 tcttaaagac tggagagagc caacgaaaag acggaagata cttatacaaa tatatagatt
    16741 catttggaga accgcaattt gtttactcgt ggaaacttgt ggctacagac cgagtaccag
    16801 caggaaagcg tgattgtatc tcacttagag agaaaatcgc agagttacag aaagacattc
    16861 atgatggtat tgatgttgta ggaaagaaaa tgacactctg ccagctttac gcaaaacaga
    16921 acgctcaaag accaaaggtt agaaaaaaca ctgaaactgg acgcaaatat cttatggata
    16981 ttttgaagaa agacaagtta ggtgtaagaa gtattgacag tattaagcca tcagacgcta
    17041 aagaatgggc tattagaatg agtgaaaatg gttatgctta tcaaaccatc aataactaca
    17101 aacgttcttt aaaggcttca ttctatattg ctatacaaga tgattgtgtt cggaagaatc
    17161 catttgactt tcaactgaaa gcagttcttg atgatgatac tgtccctaag accgtactaa
    17221 cagaagaaca ggaagaaaaa ctgttagcct ttgcaaaagc tgataaaacc tacagcaaaa
    17281 attatgatga aattctgata ctcttaaaaa caggtcttcg tatttcagag tttggtggtt
    17341 tgacacttcc agatttagat tttgagaatc gtcttgtcaa tatagaccat cagctattga
    17401 gagatactga aattgggtac tacattgaaa caccaaagac caaaagtggc gaacgtcaag
    17461 ttcctatggt tgaagaagcc tatcaagcat ttaagcgagt gttagcgaat cgaaagaatg
    17521 ataagcgtgt tgagattgat ggatatagtg atttcctctt tcttaataga aagaactatc
    17581 caaaagtggc aagtgattac aacggcatga tgaaaggtct tgttaagaaa tacaataagt
    17641 ataacgagga taaattgcca cacatcactc cacatagttt gcgacataca ttctgtacca
    17701 actatgcaaa tgcaggaatg aatccaaagg cattacagta cattatggga catgctaata
    17761 tagccatgac gctgaactat tacgcacatg caacattcga ttctgcaatg gcagaaatga
    17821 aacgcttgaa taaagagaag caacaggagc gtcttgttgc ttagtagtac aaatgaattt
    17881 actacttatt taccacttct gacagctaag acatgaggaa atatgcaaag aaacgtgaag
    17941 tatcttccta cagtaaaaat actcgaaagc acatagaata aggctttacg agcatttaag
    18001 aaaatataaa aagataatta gaaatttata ctttgt
//
LOCUS       DANBWH010000035        17890 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.036, whole genome shotgun sequence.
ACCESSION   DANBWH010000035 DANBWH010000000
VERSION     DANBWH010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17890
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.036"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(263..2074)
                     /locus_tag="PPY92_000819"
     CDS             complement(263..2074)
                     /locus_tag="PPY92_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDJ2624838.1"
                     /translation="MDTKDFKRLEKMYSPRYLPGLDGLRAFAVIGIIIYHLNAQWLSG
                     GFLGVDTFFVISGYLITSLLISEYYRTQKIDLLEFWKRRLKRLIPAVLFLICVVLTFT
                     LIFKPELIIQMKRDAIAAIFYVSNWWYISQNVDYFNQFAIEPLKHLWSLAIEEQFYLL
                     FPLVITFLLHRFKPRNIIQTLFIVSLISLGLMIVIHFITGDNSRVYFGTDTRLQTLLL
                     GCILAFIWPPFALKKDISKKIVVSLDIIGISGFAVLMTLFFIVGDQDQWIYNGGFYII
                     SFATLFIIAIAVHPSSLFAKFLSMKPLLIIGKRSYSLYLWHYPIIVFVNSYYVQGQIP
                     VYVYIIEILLTALMAEISYRFIETPIRKKGFKAFAFLPKKKGQFARTVLVILLLVPSI
                     VVLSGQFDALGKQHEAEKKEKKTEFKTTKKKVVKKDKQEDKQTANSKEDIKKSSPLLI
                     GDSVMVDIGNVFTKKIPNAQIDGKVGRQLVDATPIVKSQYKDYAKKGQKVVVELGTNG
                     AFTKDQLNELLDSFGKADIYLVSIRVPRDYEGRINKLIYEAAEKRSNVHLVDWYKASA
                     GHPEYFAYDGIHLEYAGSKALTDLIVKTMETHATNKK"
     gene            complement(2587..3075)
                     /locus_tag="PPY92_000820"
     CDS             complement(2587..3075)
                     /locus_tag="PPY92_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDJ2624839.1"
                     /translation="MAINIIEYNRSYKEELIEFILSIQKNEFNIKIDRDDQPDLENIE
                     HNYLNSGGQFWLAINNHQNIVGTIGLIRLDNNMSALKKMFVDKGYRNLKIGKKLLDKV
                     IMTCKEQNIDGIYLGTIDKFISAQYFYSNNGFREIKRGDLPSSFPKLDVDNRFYYRNL
                     KD"
     gene            complement(3351..4052)
                     /gene="isaA"
                     /locus_tag="PPY92_000821"
     CDS             complement(3351..4052)
                     /gene="isaA"
                     /locus_tag="PPY92_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase IsaA"
                     /protein_id="HDJ2624840.1"
                     /translation="MKKTIMASSLAVALGVTGYAAGTGHQAHAAEVNVDQAHLVDLAH
                     NHQDQLNAAPIKDGAYDIHFVKDGFQYNFTSNGTTWSWSYEAANGQTAGFSNVAGADY
                     TTSYNQGSDVQSVSYNAQSSNSNVEAVSAPTYHNYSTSTTSSSVRLSNGNTAGATGSS
                     AAQIMAQRTGVSASTWAAIIARESNGQVNAYNPSGASGLFQTMPGWGPTNTVDQQINA
                     AVKAYKAQGLGAWGF"
     gene            complement(4660..5715)
                     /locus_tag="PPY92_000822"
     CDS             complement(4660..5715)
                     /locus_tag="PPY92_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HDJ2624841.1"
                     /translation="MTSIKTITPKDFIFRVLSGVAIGIVAGLVPNAILGEIFKYFMDY
                     HPIFKTLLGVVVAIQFTVPALIGALVAMKFDLSPLAIAVVASAAYVGSGAAQFKNGAW
                     MITGIGDLINTMITAAIAVLFILLIQHRVGSMALIVFPTVVGGISGAIGVLILPYTKM
                     ITTAIGNMVNGFTELQPIVMSILISMVFSLIIISPLSTVAIAFAIGITGLAAGSASIG
                     ISATEAVLIIGTSKVNRLGVPLSVFFGGVKMMIPNMVKYPILMLPILTTAIVSGLVSA
                     LVGIHGTKESAGFGFIGMVGPINAFKFMEVDSAWLSVLLIVVAFFVVPFVTAWLADII
                     YRKVFRLYTNDIFKFMG"
     gene            complement(5842..5970)
                     /locus_tag="PPY92_000823"
     CDS             complement(5842..5970)
                     /locus_tag="PPY92_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624842.1"
                     /translation="MDFKNEDKIKVYCNNMFEKLPISLSKNSEFCDILFFIVLSYR"
     gene            6125..6694
                     /locus_tag="PPY92_000824"
     CDS             6125..6694
                     /locus_tag="PPY92_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDJ2624843.1"
                     /translation="MMESKSIDPRIVRTKQLLVDAFLKISREKKLSQITVKDITDIAT
                     LNRATFYAHFTDKEDLLDYTLSVTILKDLNDNLSISNVINEKVLRNIFISIASYIKDA
                     AKSCELNSEAFCNKAHQRINNELEDIFAIMLENSYPEHQRDIIVNSASFLAAGISGLA
                     LHWFNTSQETADVFIDRNLPFLIHHIAHF"
     gene            6892..7392
                     /locus_tag="PPY92_000825"
     CDS             6892..7392
                     /locus_tag="PPY92_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624844.1"
                     /translation="MTSIFVFAYLNNTSGNMLFRQQMICACLLANVRHSSIVCLNNIC
                     CIVDMRCLNTKIGGIDVSETDEPQGFERTHNILNINQSSLGVVTYITNKLKSTLKQHI
                     IIARGKKRIDYRLSYNFYTTYYDNVEIKKIDCEYTYAMPIAILIRHTMSFDNAHFFAQ
                     LRHPVL"
     gene            7612..7842
                     /locus_tag="PPY92_000826"
     CDS             7612..7842
                     /locus_tag="PPY92_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HDJ2624845.1"
                     /translation="MKILDRINELANKEKVQPLTVAEKQEQHALRQDYLSMIRGQVLT
                     TFSTIKVVDPIGQDVTPDKVYDLRQQYGYIQN"
     gene            complement(8144..8521)
                     /locus_tag="PPY92_000827"
     CDS             complement(8144..8521)
                     /locus_tag="PPY92_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDJ2624846.1"
                     /translation="MIQSMWFNLHVQDLEKSAQFYKALGFKINRNPQMLDKMVGIQIG
                     QTTVILIENKHFQNVSQQSLNTEPNEVMISLGVNTNEEVDQLVNKVKEAGGAVVQEPT
                     VSQGFYGAMFKDLDGHHFNFLVC"
     gene            complement(8538..9359)
                     /locus_tag="PPY92_000828"
     CDS             complement(8538..9359)
                     /locus_tag="PPY92_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HDJ2624847.1"
                     /translation="MKDILVIGATGKQGNAVVKQLLEDGWYVSALTRNKNNRKLSDIG
                     HPHLSIVEGDLSDNVSLQSAMKGKYGLYSIQPIVKDDVSEELRQGMKIIEIAEQENIQ
                     HIVYSTAGGVNRNRTGPHFEVLAKIENRLMESNINATVIKPSFFMDNFLRIAKVEDER
                     ITLPEFINPNIKFTMISSIDIAKIASYIFAHPQSFTHQSIEIGSDEVTLSEAATIFSE
                     VTGKSTVIEGEFVSGVAEKQWLEEKGYEVDFELMAEINPTRLSLSDWLKVQNYNK"
     gene            complement(9379..9678)
                     /locus_tag="PPY92_000829"
     CDS             complement(9379..9678)
                     /locus_tag="PPY92_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HDJ2624848.1"
                     /translation="MSLNKEQRRITAEELQAHFEESTLSVQMIAEKLNVTTEDVEKVL
                     AMTAPLGIFSHQLQRFIHLVWDVRDVINDNIKGNGQTPEPYTYLKGEKEDYWFLR"
     gene            9809..10366
                     /locus_tag="PPY92_000830"
     CDS             9809..10366
                     /locus_tag="PPY92_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDJ2624849.1"
                     /translation="MRKDAKENRQRIEEIAHKLFDEEGVENISMNRIAKELGIGMGTL
                     YRHFKDKSDLCYYVIQRDLDIFITHFKQIKDDYHSNYEVMQVSLDYLLQFKIDNKALL
                     QCIEAGNNKLRFYQSAFYQELFDFYYDLFKSDDDTYTKFKTDMLLQSLSTSVFAFQIE
                     HRHISIEAYRNYLLNIYLDEVERND"
     gene            10359..11063
                     /locus_tag="PPY92_000831"
     CDS             10359..11063
                     /locus_tag="PPY92_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDJ2624850.1"
                     /translation="MTKIVLITGGNKGLGYASAEALKALGYKVYIGSRNDVRGQQASQ
                     KLGVHYVQLDVTSDYSVKNAYNMIAEKEGRLDILINNAGISGQFSAPSKLTPRDVEEV
                     YQTNVFGIVRMMNTFVPLLEKSEQPVVVNVSSGLGSFGMVTNPETAESKVNSLAYCSS
                     KSAVTMLTLQYAKGLPNMQINAADPGATNTDLVGDFSNNSKHVSEGIKPIIQLATIGA
                     DGPTGTFINGDGEMPW"
     gene            11161..12171
                     /locus_tag="PPY92_000832"
     CDS             11161..12171
                     /locus_tag="PPY92_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDJ2624851.1"
                     /translation="MKSITFEEHYVIEDIQKETMNAISADPKGVPMKVMLEGLEKKTG
                     FTNADELSHHDERIQFMNNQDVQIQVLSYGNGSPSNLVGQKAIELCQKANDQLANYIA
                     QYPNRFVGFATLPINEPEAAAREFERCINDLGFKGALIMGRAQDGFLDQDKYDIIFKT
                     AENLDVPIYLHPAPVNSDIYQSYYKGNYPEVTAATFACFGYGWHIDVGIHAIHLVLSG
                     IFDRYPKLNMIIGHWGEFIPFFLERMDEALFAEHLNHSVSYYFKNSFYITPSGMLTKP
                     QFDLVKKEVGIDRILYAADYPYIEPEKLGVFLDELGLTDEEKEKISYTNGAKLLGLSS
                     NN"
     gene            12191..13021
                     /locus_tag="PPY92_000833"
     CDS             12191..13021
                     /locus_tag="PPY92_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDJ2624852.1"
                     /translation="METLELQGAKLRYHQVGQGPVLIFIPGANGTGNIFLPLAEQLKD
                     HFTVVAVDRRDYGESELTEPLPDSASNPDSDYRVKRDAQDIAELAKSLSDEPVYILGS
                     SSGSIVAMHVLKDYPEVVKKIAFHEPPINTFLPDSTYWKDKNDDIVHQILTEGLEKGM
                     KTFGETLNIAPIDAKMMSQPADTEEGRIEQYKRTMFWSEFEIRQYTHSDITLDDFTKY
                     SDKITLLNGTDSRGSFPQDVNFYINKETGIPIVDIPGGHLGYIQKPEGFADVLLNMWG
                     "
     gene            complement(13168..14058)
                     /locus_tag="PPY92_000834"
     CDS             complement(13168..14058)
                     /locus_tag="PPY92_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDJ2624853.1"
                     /translation="MKIVIIGGFLGGGKTTVLNHLLAESLKESLKPAVIMNEFGKMSV
                     DGALVSEDIPLSELTEGCICCAMKADVSEQLHQLYLKEQPDIVFIECSGIAEPVSVLD
                     ACLTPILAPFTTITHMIGVIDASMYKHIKSFPKDIQGLFYEQLAYCSVLFVNKIDSAD
                     VETTSKLLKDLEVINPEADIQVGMHGSVTLPISVRQMTATSDNKHESLHKTINHQFVQ
                     SPVTCTKAEFIKRLACLPSHIYRLKGFMTFEDTTHTYLIQFTQGQYELTPVAFSKKVP
                     EYLVLIGKGISKEDYQCLEQ"
     gene            complement(14155..15495)
                     /locus_tag="PPY92_000835"
     CDS             complement(14155..15495)
                     /locus_tag="PPY92_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HDJ2624854.1"
                     /translation="MLEVKYDMRTGHYTPIPHEPHYLVISHADKLTATEKAKLRLLII
                     KQKLDISLAESVVSSPIASEHVIEQLTLFQHERRHLRPKISATFLAWLLIFLMFALPI
                     GIAYQFSDWFQNQYVSAWIEYLTQTTLLNHDILQHILFGDYGVLSLGTYSLVWALPVV
                     ILISLSTAIIDQTGLKSWMIWAIEPSMLWIGLQGNDIVPLLEGFGCNAAAISQAAHQC
                     HTCTKTQCMSLISFGSSCSYQIGATLSIFSVAGKSWLFMPYLILVLLGGILHNRIWLK
                     KNDQQLSVPLPYDRQLHMPNIRQMLLQMWQNIQMFIVQALPIFITICLIVSILSLTPI
                     LNVLSQIFTPILSLLGISSELSPGILFSMIRKDGMLLFNLHQGALLQGMTATQLLLLV
                     FFSSTFTACSVTMTMLLKHLGGQSALKLIGKQMVTSLSLVIGVGIIVKIVMLII"
     gene            complement(15489..16595)
                     /locus_tag="PPY92_000836"
     CDS             complement(15489..16595)
                     /locus_tag="PPY92_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HDJ2624855.1"
                     /translation="MQHHKVAIIGAGAAGIGMAITLKDFGITDVIILEKGTVGHSFKH
                     WPKSTRTITPSFTSNGFGMPDMNAISMDTSPAFTFNEEHISGETYAEYLQVVANHYEL
                     NIFENTVVTNISADDAYYTIATTTETYHADYIFVATGDYNFPKKPFKYGIHYSEIEDF
                     DNFNKGQYVVIGGNESGFDAAYQLAKNGSDIALYTSTTGLNDPDADPSVRLSPYTRQR
                     LGNVIKQGARIEMNVHYTVKDIDFNNGQYHISFDSGQSVHTPHEPILATGFDATKNPI
                     VQQLFVTTNQDIKLTTHDESTRYPNIFMIGATVENDNAKLCYIYKFRARFAVLAHLLT
                     QREGLPAKQEVIENYQKNQMYLDDYSCCEVSCTC"
     gene            complement(16928..17224)
                     /locus_tag="PPY92_000837"
     CDS             complement(16928..17224)
                     /locus_tag="PPY92_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HDJ2624856.1"
                     /translation="METIGSIIYLKEGSQKLMIINRGPIVEIENQKYMFDYSACKYPI
                     GVVEDEIYYFNEENIDSVIFKGYSDQDEVRFQELFENMKQNLDSEIQRGEVTPQ"
     gene            complement(17212..17580)
                     /locus_tag="PPY92_000838"
     CDS             complement(17212..17580)
                     /locus_tag="PPY92_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624857.1"
                     /translation="MSNKLDGINKMITAKHKQMDDLYDEKREVKALIDESDELNHSIE
                     QLYQHLGDRYHSSNMASRMEQFRDEFHFAKRRSTEALYEQQQQIQHGIRKAEEEMIDL
                     EMRRNVEIETVTKEENKWKQ"
     gene            complement(17595..17873)
                     /locus_tag="PPY92_000839"
     CDS             complement(17595..17873)
                     /locus_tag="PPY92_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04197 family type VII secretion effector"
                     /protein_id="HDJ2624858.1"
                     /translation="MMSTVQSDIFKTNSASSSIKSAVETCNNVSKPDKDESTTVSGNN
                     NAHSVIDDLISKNQSVAEAIRNASDSIQKVGEEFNQTDVMIGNEIGKN"
BASE COUNT         5879 a         3243 c         2725 g         6043 t
ORIGIN      
        1 ataattataa cttttatagt tttgcgacaa aacaaactat aacagagatt aggtgtcttg
       61 cgggctttgt tatcataatg taatctgttc ctaataaagc ttcattgaat gtaatatacc
      121 aattagaatg tttacaacac tttttctgca ccgttaacat gggatttgaa aataaaaaag
      181 cactaaactt ttaatgaaat acatcatttt tgataataaa tatcagaagt aatgtaatat
      241 ttcaaaagtt tagtgcatca agttatttct tatttgtagc atgtgtttcc atcgttttta
      301 caatcaaatc agtcagcgct ttactacctg catattctaa gtgaataccg tcatatgcaa
      361 agtattccgg atgacctgca gaagctttat accaatcgac tagatgtaca ttagagcgct
      421 tttcagctgc ctcataaatt aatttattta ttctaccttc ataatctcta ggtactctaa
      481 tagaaactaa atatatgtct gcttttccaa aactatccaa tagttcattt aattgatctt
      541 tcgtaaatgc cccatttgta ccaagctcta ctacaacttt ttgacctttt ttagcatagt
      601 ctttatattg cgatttcaca attggtgtag catcaacgag ttgccgtcca actttaccat
      661 caatttgtgc atttggtatt ttcttagtaa agacattacc aatatccacc atgaccgagt
      721 caccaattaa tagtggtgat gactttttaa tatcctcttt gctattcgct gtctgcttat
      781 cctcttgctt atctttttta acgacttttt tcttcgttgt tttaaattcc gtcttcttct
      841 ctttcttctc ggcttcatgt tgtttgccaa gtgcatcaaa ctgtccactg agcacaacga
      901 tagacggaac caataatagg ataactaaca ctgttctagc aaattgcccc ttctttttag
      961 gtaaaaatgc aaaagcttta aatccttttt tacgtatagg tgtttcaata aagcgatacg
     1021 aaatttcagc cattaacgct gttaacaaaa tttctataat ataaacgtat accggtattt
     1081 gtccttgtac gtaataactg ttcacaaaaa caatgatagg ataatgccat aaatataagc
     1141 tatatgatcg tttacctata attagtaaag gtttcatact taaaaattta gcaaataaac
     1201 tagaaggatg taccgcaatt gcaataatga ataaagttgc aaatgatata atgtaaaatc
     1261 ctccattata gatccattga tcttggtcgc caactataaa gaacaaagtc attagaaccg
     1321 caaaaccaga tatccctata atatctaatg atacgacaat ctttttagaa atatcttttt
     1381 tcaaagcaaa cggaggccaa ataaatgcta atatacaacc aagcaataaa gtttgcagtc
     1441 gtgtatctgt cccaaaatac acacgtgaat tatctccagt gatgaaatga atcactatca
     1501 taagtcctaa agaaatcaac gatacaataa atagcgtttg aataatattt ctcggtttaa
     1561 atctatgtaa taagaacgtg ataaccaatg ggaaaagcaa gtaaaattgt tcttcaatgg
     1621 ctaaagacca taaatgtttg agtggttcaa tagcaaattg gttaaaataa tctacattct
     1681 gtgaaatgta ccaccagttt gaaacataga atatagctgc aatagcatct cgtttcattt
     1741 gtataatcaa ttccggttta aatatcagtg tgaacgtaag cacgacacag attaaaaata
     1801 acactgccgg aatgagtcgt ttcaatcgtc gcttccaaaa ctctagcaaa tcgatttttt
     1861 gcgtccgata atactcactt atcaacaaac ttgttattaa ataacctgaa ataacgaaga
     1921 atgtatctac tcctaaaaag cccccactta accattgtgc attcaagtga taaataatga
     1981 ttcctataac tgcgaatgcc ctcaatccat ctaatccggg taagtatcgc ggggaataca
     2041 ttttttctaa acgtttaaag tcttttgtat ccatgttaat aaacgcccca tttatttttc
     2101 tctattttgt agcatatcac aatatttttg aaaataaaat attgcactga ttttcattaa
     2161 ttgatttaac ccttaattaa gatagtttta aattttttat taagtagaaa acaattatta
     2221 cagttaattt cattactgca aaccacatat aaatttgtcg attttactac ataacataga
     2281 ttatcataga ttcttgaatt tttagcaaaa taactgttat tttcattata tttttacaaa
     2341 aaaaggttcg ttttatattt tatgcatctt actgtaacag aatcattaag atatgctatt
     2401 cgaatttact ttttcaaaat ttctataatg aataaattac catgtattga aaataaagcg
     2461 aaatgcagcc tatcctctaa tttaaaccaa gcgatatatc acgtcagcct ttatatttaa
     2521 acgctatgtg tcacttttaa aatgaatatt actaagattg tcataccaat tattcattgc
     2581 atcgaattaa tcttttaaat ttctgtaata aaacctatta tctacatcta atttaggaaa
     2641 actacttggt aaatctcctc gcttaatttc acggaagcca ttattagaat aaaaatactg
     2701 tgcactaata aatttatcaa ttgttcctaa ataaatacca tcgatatttt gttctttaca
     2761 tgtcattata actttatcta aaagtttttt acctattttt aaattcctat aacctttatc
     2821 aacaaacatt tttttaagtg cagacatatt attatctagt ctaatcaaac ctatagtacc
     2881 aacaatattt tgatgattgt ttattgcaag ccaaaattgc cctccactat tcaaatagtt
     2941 atgttcgatg ttctccaaat caggttgatc atctctatca atttttatat taaattcatt
     3001 tttttgaatc gataaaataa actcgattag ctcttcctta taagacctat tatattcaat
     3061 tatgtttata gccattttta tctccttttt catttaattt aatcataaaa tgcgcgttta
     3121 gtttgtatct agtgtactca gtacagcctc aaatgaagtt ttattccact tggcacttaa
     3181 taaagacaag tattttagca gtaatacaat aaagtccaat aaatttccct aacttcaata
     3241 tccacttttt aagaaatgta tttttaatta ataaaaaaac tctccccaat ttctatggga
     3301 agagctatat atttaatgtc taaacattac ttttatttat tatgaaggaa ttagaatccc
     3361 caagcaccta aaccttgtgc tttgtatgct ttaacagctg cgttgatttg ttggtcaaca
     3421 gtgtttgtcg gaccccaacc tggcatagtt tggaataaac ctgaagcacc tgatgggttg
     3481 taagcattta cttgaccatt tgattcacga gcgatgattg cagcccatgt agaagctgaa
     3541 acaccagtac gttgagccat gatttgagct gctgatgaac cagtagcacc tgcagtatta
     3601 ccattgctta atctcactga acttgaagta gttgaagtgc tgtagttatg gtaagttgga
     3661 gctgaaacag cttcaacgtt tgagttactt gattgtgcat tgtagcttac tgattgtaca
     3721 tctgaacctt ggttgtatga agtagtgtag tctgcacctg caacgtttga gaaaccagca
     3781 gtttgaccat tagctgcttc atagctccat gaccatgtag taccatttga agtaaagtta
     3841 tattggaaac catcttttac aaagtggatg tcatatgcac catctttgat tggagctgca
     3901 tttaattgat cttggtgatt atgcgctaag tcaactaagt gtgcttgatc aacgtttact
     3961 tcagcagcgt gtgcttgatg tcctgtacct gctgcgtaac ctgttacacc taatgccact
     4021 gctaatgatg atgccataat tgtctttttc atagtaaaaa atcctccagt aataattgta
     4081 agtttatgtt tttagtaatt atattttgaa tttgaatgtc gtagtgcaag tttaaattgt
     4141 cttttatttc tttcaacggt actcactata tcacaaaaaa ccagccagta aattacactt
     4201 tctttacaaa acattacaat atcaagtgtt atttgtaatg ttgaaatatg gctgttttat
     4261 actgtaatgt gaaatatgtg ccctttagaa tccaatcaac ccttgaaata gtctttaaca
     4321 cataagattt ttactatatt tagctcaact attacagctt tcgtaatatt acagattgta
     4381 tttttgttac atagctgtaa tatatctgac atgtaacttc tcttattttc gtacattaaa
     4441 cgcaattaaa aagcaatcaa cacatatgtt tctgcacatg tattgattgc tattattgtt
     4501 gtatattcaa agttttaaaa cacacatctt ttgtgaatta tcttatcttt tataagcaca
     4561 aataaactgc aactcaatta tattgctaaa ctttattctc gcaattcaca ataacattaa
     4621 ataatttttg gtctaatatt ttcaaaaaac atactgtcct tatcccatga atttaaaaat
     4681 atcattagta tataaacgaa acactttacg ataaatgata tctgcaagcc aagctgttac
     4741 aaatggcaca acaaagaacg ctactacaat tagtaagaca ctcaaccaag cagaatcaac
     4801 ctccataaat ttaaatgcat taatcggtcc taccattcct ataaaaccaa atccagctga
     4861 ttctttcgtt ccatgaatac ctactaatgc tgataccaaa cctgatacaa tggctgttgt
     4921 caatattggt aacataagaa ttggatattt caccatatta ggtatcatca ttttaacgcc
     4981 tccaaagaag acggataacg gcacccctaa acgattcact ttacttgtac caattatcaa
     5041 tactgcttca gtcgcggaga taccaattga cgctgatcca gctgctaaac ctgtaatacc
     5101 tatcgcaaag gcaatggcca cagttgatag tggcgaaata ataataagac taaataccat
     5161 tgaaatcaaa atactcatga caatcggttg taattctgta aaaccattaa ccatattacc
     5221 gatggctgtt gtaatcattt tcgtatacgg caatattaaa acaccaattg cacctgaaat
     5281 accgccaaca actgttggga atacaatcaa tgccatacta cctacacgat gttgaataag
     5341 taaaatgaac aacactgcaa tcgctgctgt aatcattgta ttaattaaat caccaatacc
     5401 cgtaatcatc caagcaccat ttttaaactg cgctgcaccg cttcctacat atgctgcact
     5461 tgccacaaca gcaattgcta atggcgatag gtcaaatttc atggcaacca atgcaccaat
     5521 caaagcaggt actgtaaatt gaattgcaac gacaacgcct aataacgttt taaaaatcgg
     5581 atgataatcc ataaagtatt taaaaatttc tccaagtatc gcattaggaa ctaaacccgc
     5641 aacaatacct atggcgacac ctgataaaac tctaaatata aaatctttgg gtgtaattgt
     5701 tttaattgat gtcataatat catccttcca tttatgtata tacatctgta tgcaaataat
     5761 aaagagcctt aagttataag ctgccactag cttaaattct aagatgtgca tgccgatgtt
     5821 gttatattta ggctagcagt atcatctata actcaagact atgaaaaata gtatatcaca
     5881 aaattctgaa tttttagata aagaaattgg caatttttca aacatattgt tacaatacac
     5941 ttttatttta tcttcatttt taaaatccat taatacaata gaagaaagac attcaaatgc
     6001 ttaccaaaaa ggtacattat ttgttaggag cgtatcaaca cttacatatc atcaacacaa
     6061 ttgacaatat aatagaagat actgataata agtgttaaaa caacagatgt taggtagtga
     6121 acaaatgatg gaaagtaaat ccatagatcc aagaatcgtt agaaccaaac aattgcttgt
     6181 cgatgctttt cttaaaattt ctagagaaaa gaaattatct caaattacag ttaaagatat
     6241 cactgatatt gctacgctaa atcgtgcaac attttacgct catttcactg ataaagaaga
     6301 cctcctagac tacacattat ctgtaaccat tttaaaagac ttgaatgata acttgagcat
     6361 ttctaatgtc attaatgaaa aggtgctgcg taatattttc atttcaattg cgagttatat
     6421 caaagatgct gcaaagtctt gcgaattaaa tagtgaagca ttttgcaaca aagcacatca
     6481 acgtattaat aatgaattag aagatatttt tgcgattatg ttagaaaaca gctatccgga
     6541 gcatcaacga gatatcattg taaatagtgc gagtttttta gcagctggta tctcaggctt
     6601 agcattacat tggtttaaca cgagtcaaga gacagccgat gtgtttatcg atcgcaacct
     6661 tccattttta attcatcata tagcacattt ttaataaaac ttggtattta gtcatgcatc
     6721 ttgaaatcac tatgtgactt aggttcatac ttgtacacac aataaaattt aacatattac
     6781 gattgattag ccgtgtctag gacataaatc aacgtcctat actctacaat gtcatattag
     6841 cagtcgttaa ctgaatgaaa ataagcttgt cattaaaaca tatagatttt agtgacaagc
     6901 atttttgttt ttgcgtactt aaacaacact tcaggcaata tgttgtttag gcaacaaatg
     6961 atatgtgcgt gtttattggc aaacgtacga catagtagta tagtatgtct aaacaacata
     7021 tgttgcatag ttgatatgcg ttgtttaaat actaagatag gagggattga cgtgagcgag
     7081 acagatgaac ctcaggggtt tgaacgcacg cataatatat taaatattaa tcagagtagt
     7141 ctgggtgtag tgacatacat tacaaataaa ttaaagtcga cgttgaagca acacataata
     7201 attgctcgtg gtaaaaagcg aatcgactat cgactgtcgt ataactttta cactacgtat
     7261 tatgataatg tagaaatcaa gaaaatcgac tgtgaatata cctatgctat gcccattgca
     7321 attttaataa gacacacgat gtcattcgac aatgctcatt tctttgctca gttacgtcat
     7381 cctgtcttat aaaacaacat tgcagacatg tatatcaaac gacacttcaa taacatcact
     7441 ttgccatcgt actactatta aaatcgtgtc tcaaacccct tattttaatt ccaaaaatct
     7501 gctggtcaaa agaccgagaa actaaaaaca ttactggatg tgttgataaa ttatcttata
     7561 gaaataaact caaaatatat caacacttga ttctaaggag gatatgacaa tatgaaaatt
     7621 ttagatagaa ttaatgaact tgcaaataaa gaaaaagtcc aaccacttac tgtagctgaa
     7681 aaacaagaac aacatgcatt gcgtcaagac tacttaagta tgatccgagg acaagtatta
     7741 acaacatttt ccacaataaa agtggttgat ccaatcggtc aggatgtcac accagataaa
     7801 gtttatgatc ttcgccaaca atacggttat attcaaaatt aatatttgct cacgaggtat
     7861 tgcacttaag gtgccaactg acctcataaa caaagcccat actgattgaa gacactaatg
     7921 tgtccaggct gggacataaa tcaatgttct atgctctacg aagttatatt ggcagtagtt
     7981 gactgaatga aaatgcgctt gtaacaagct tttttcaatt ctagtcaacc ttgccggggt
     8041 gggacgacga aataaatttt gcgaaaatat catttctgtc ccactcccac attaaacttc
     8101 atctctgtat gggcttttta tttattcttt tgagaatttc attttagcag actaaaaaat
     8161 taaaatggtg tccatcaaga tctttgaaca ttgcaccata aaacccttgg cttactgttg
     8221 gttcttgaac gactgcgcca cccgcttctt tcactttatt caccaactgg tcaacttctt
     8281 catttgtgtt cacacctaga gaaatcatca cttcatttgg ttcagtatta aggctttgct
     8341 gacttacatt ttgaaaatgc ttgttttcta ttaaaattac ggttgtttga cctatttgaa
     8401 taccgaccat tttatctaac atttgtgggt ttctatttat tttaaatcct aacgctttat
     8461 aaaactgtgc gctcttttct aaatcttgca catgcaaatt aaaccacatt gattgaatca
     8521 tgattgcacc ccattcatta cttattatag ttttggactt taagccaatc acttaatgat
     8581 aatcttgttg gatttatttc agccattaat tcaaagtcta cttcataacc tttttcttcc
     8641 aaccattgct tttctgcaac accactaaca aattctcctt ctataacagt agatttacct
     8701 gtcacttcac taaaaattgt tgctgcttca cttaatgtaa cttcatcgga accaatctct
     8761 attgattgat gcgtaaagct ttgtggatgt gcaaaaatat acgatgcaat tttagctata
     8821 tcaatagaag aaatcattgt gaattttata ttcggattaa taaattctgg taatgtaata
     8881 cgttcatctt cgactttagc aatgcgtaaa aaattatcca taaagaatga tggtttgata
     8941 actgttgcat ttatattaga ttccattaat ctattttcta tttttgctag tacttcaaag
     9001 tgtgggccag ttcgatttcg attaacccct cccgcagtac tatacacaat atgttgaata
     9061 ttttcttgct cagctatttc aattatcttc ataccttgtc ttaattcttc gctaacatca
     9121 tctttaacga ttggctgaat actgtataag ccatacttcc ctttcatcgc tgattgcaaa
     9181 ctaacattat cactcagatc accttcaacg attgataaat gcggatgtcc tatgtctgaa
     9241 agtttacgat tattcttatt tctagttaat gcacttacat accatccatc ctctaacaac
     9301 tgttttacaa ctgcattacc ttgcttccct gttgcgccta ttactaaaat atctttcatt
     9361 ttagatccac tcctcttttt atcttaaaaa ccaatagtcc tctttttcac cttttaaata
     9421 cgtatatggt tctggtgttt gtccatttcc tttaatattg tcgtttatta catctctgac
     9481 atcccatact aaatgaataa atcgttgtaa ttgatgacta aaaatgccta gtggcgctgt
     9541 catagctaat actttttcca catcttctgt agtgacattc agtttttcag caatcatttg
     9601 aaccgataag gtagattctt caaaatgtgc ttgcaactct tcagctgtga tgcgtctttg
     9661 ctctttattt aaagacatta tcaacactct tttcttttat gatatttaat agttattgca
     9721 aattcattgc taactttctt aacacgtttt ataataaacg gatagttatc cgtttgtcaa
     9781 ttttagagaa ttttaagtag gtgtaatcat gcgaaaagat gcaaaagaga atagacaacg
     9841 aattgaagaa atagcgcaca aactttttga tgaagaaggc gttgaaaaca ttagtatgaa
     9901 tcgtattgct aaagaactag gtatcggtat ggggacattg tatcgacatt tcaaagataa
     9961 aagcgattta tgctactacg tcatacaaag agaccttgat atttttatca ctcattttaa
    10021 gcaaattaaa gatgactatc attctaatta tgaggttatg caagtgtcgt tagattatct
    10081 tttacaattt aaaatcgata ataaggcact actgcaatgt attgaagcag gcaacaataa
    10141 attacgtttt taccaaagcg cattttatca agaattattt gatttttatt atgacttatt
    10201 taaaagcgat gacgacactt acacgaagtt caaaacagat atgctgttgc aatcattatc
    10261 aactagtgta tttgccttcc aaatagagca tcgccatatt tcaattgaag cgtacagaaa
    10321 ttatttatta aacatttatt tagatgaggt ggaacgtaat gactaaaatt gtgttaatca
    10381 caggaggcaa taaagggtta ggctatgcaa gtgcagaggc acttaaagca ttgggttaca
    10441 aagtttatat aggttctcgg aatgatgtaa gaggacaaca agcatcacaa aaattaggtg
    10501 ttcattatgt acagttagat gttacaagtg actactcagt caaaaatgct tataacatga
    10561 ttgctgaaaa agaaggccgt cttgatatcc ttattaacaa tgcaggtata tctggtcaat
    10621 tttcagcacc ttccaaattg acacctcgtg acgttgaaga agtatatcaa acgaatgtat
    10681 tcggtatcgt acgaatgatg aatacattcg tccctctctt agaaaaatct gaacaacctg
    10741 ttgtcgtcaa cgtatcaagt ggtttaggtt catttggaat ggttacaaac cccgaaacag
    10801 ctgaatctaa agtgaattca ttagcttatt gttcgtctaa atcggcagta acaatgctaa
    10861 cgctacaata cgctaaagga ttacctaaca tgcaaatcaa tgctgctgac ccaggcgcta
    10921 caaatactga tttagttggt gattttagta ataattccaa acacgtttct gaaggaatca
    10981 agccaatcat tcaattagca acgattggtg cagatggccc aacaggtaca tttattaatg
    11041 gcgatggaga aatgccttgg tagatcaatt ttatgttaca aacattgaaa ttagaggtat
    11101 ataaaaaatg cttctgcaat agatgcaata tacatgcaaa acaatatgga ggcgaattaa
    11161 atgaaaagta ttacgtttga agaacattat gtcattgaag atattcaaaa agaaacgatg
    11221 aatgcgatat cagcagatcc taaaggtgta ccgatgaaag taatgttaga aggccttgaa
    11281 aaaaagacag gttttacaaa tgccgacgaa ttatcacatc atgatgaacg tatccaattt
    11341 atgaataatc aagacgttca aattcaagtc ttatcttatg gaaatggatc tccttcaaat
    11401 ttagttggtc agaaagccat tgaattatgt caaaaagcaa atgatcaatt ggcaaactat
    11461 attgcccaat atcccaatcg ctttgtaggc tttgcaactt tacctatcaa tgagcctgaa
    11521 gccgcagcgc gagaattcga acgttgcatc aatgatttag gattcaaagg tgcgctcatt
    11581 atgggacgtg cacaagatgg ttttcttgat caagacaaat atgacattat tttcaaaaca
    11641 gctgaaaatt tagacgtacc gatttatcta catcccgcgc cagttaacag tgacatttat
    11701 caatcatact ataaaggaaa ttatcctgaa gtaactgcgg caacatttgc ttgttttggt
    11761 tatggttggc acattgatgt cggaattcat gcaatacatc tagtattatc tggcattttt
    11821 gatcgttatc caaagttaaa tatgattatt ggacattggg gtgagtttat cccattcttc
    11881 ttagaacgta tggatgaagc tttattcgct gaacatttga accactctgt aagctattac
    11941 tttaaaaata gtttttatat cacaccaagt ggcatgttaa cgaagccaca gtttgattta
    12001 gtcaagaaag aagtaggtat tgatagaatt ctttatgctg ctgattatcc atatattgag
    12061 cctgaaaaat taggtgtatt tttagatgaa ctgggtttaa cagatgaaga aaaagagaaa
    12121 ataagttata caaatggtgc taaattatta ggtttatcat ctaacaatta acaaatggag
    12181 gtcattgaat atggaaactt tagaattaca aggcgctaaa ttacgatacc accaagtcgg
    12241 acaaggaccc gtgctcatct ttattcctgg tgcaaacggt accggaaaca tttttctgcc
    12301 tcttgcagaa cagttaaaag accattttac tgttgtagcc gttgatcgtc gtgattatgg
    12361 agaaagcgag ttaactgaac cactccctga ttccgcttca aaccctgaca gtgattatcg
    12421 tgtcaaacgc gacgcacagg acattgccga acttgcaaag tcattaagtg atgaacctgt
    12481 ctatatatta ggttcaagtt caggttcaat cgttgcgatg catgtgttaa aagattaccc
    12541 tgaagtcgtt aaaaagattg catttcacga accaccaatt aatacatttt taccagacag
    12601 tacttattgg aaagataaaa atgatgacat tgtgcatcaa attttaacag aaggcttaga
    12661 aaaaggtatg aaaacatttg gtgaaacatt aaatatcgca ccaattgatg caaaaatgat
    12721 gtctcaacct gctgatactg aagaaggacg tatagaacag tacaaacgta cgatgttctg
    12781 gtcagaattt gaaattagac aatatacgca ttcagatatt actttggatg atttcacaaa
    12841 atacagcgat aaaatcaccc tactaaatgg gacagattct agaggctcct tcccacaaga
    12901 tgttaacttt tatataaaca aagaaactgg catacctatt gtagatattc caggcggtca
    12961 cttaggttat attcaaaaac ctgaaggctt tgctgatgta ttattaaata tgtggggtta
    13021 attgatcgtt ctctaataaa acagcttgga cagttttcaa attatatttg atggctgacc
    13081 aagcttattt ttgtttacgt ttaaacgttc aaatcaactc gttattatac atgaaggcat
    13141 gttaaccatt ctcttccact gaaaacacta ctgttccaaa cattgatagt cttcttttga
    13201 aatacccttt ccgataagca ccaaatattc tggcactttt tttgaaaatg ctacaggtgt
    13261 taattcatat tggccttgtg taaattgaat gagatacgta tgtgtggtgt cttcaaatgt
    13321 cataaaccct ttcaacctat aaatatgaga cggaaggcat gctaaacgtt ttataaactc
    13381 tgctttagta catgtgactg gtgattgcac aaattgatga tttattgttt tgtgtaatga
    13441 ttcatgttta ttgtcagaag ttgctgtcat ttgtctaact gatattggca aagtgacgct
    13501 gccatgcata ccgacttgta tatcggcctc tgggttaata acttctaaat ctttcaatag
    13561 tttgctcgtt gtttcaacat ctgctgaatc tattttatta acaaataaga cagaacaata
    13621 tgctaattgc tcataaaata agccttggat gtctttaggg aatgatttaa tgtgtttata
    13681 catgcttgcg tctattacac caatcatatg tgtaattgtt gtaaacggag ctaaaatagg
    13741 cgttaaacaa gcatctaaga cagagactgg ttctgcaatc ccactacatt caataaatac
    13801 aatgtctggt tgctctttta aatataattg atgtaactgt tctgatacat ctgctttcat
    13861 tgcgcaacag atacacccct ctgtcagttc acttaaaggt atgtcttcag atactaaggc
    13921 accatcaaca ctcattttcc caaattcatt catgatgact gctggtttca gcgattcctt
    13981 taatgattca gcgagcaaat gatttaagac agtcgtttta ccgccaccta aaaacccacc
    14041 tataataact attttcatct gcgactctcc tcttatactt catatgaata gcatattcca
    14101 agatagatct cctattgact gacatgactc tatattcagt tatagttcat ttttttaaat
    14161 aatcagcatt actattttaa caatgatgcc tacaccaata actaaagaca atgatgtcac
    14221 catttgcttt ccaattaatt ttagtgctga ctgaccacct aaatgtttca aaagcatcgt
    14281 cattgtgacc gagcacgctg taaatgttga actaaaaaac acaagtagta gtaactgtgt
    14341 tgctgtcatt ccttgtaata aggcgccttg atgcaaatta aacaagagca tgccgtcttt
    14401 tcgaatcatt gaaaataaaa tccctggtga caattctgaa gagatgccta ataacgataa
    14461 tataggtgta aatatttgtg ataaaacatt caaaattggc gttagtgata aaatactaac
    14521 aataagacag attgtgataa aaataggtag cgcttgaacg ataaacattt gtatattttg
    14581 ccacatttgt agcaacattt gacgtatatt tggcatatgt aattgcctat cataaggtag
    14641 cggaacacta agttgttgat cattcttttt caaccatatc ctattatgta agatgccacc
    14701 taaaagtact aatattaagt acggcataaa tagccatgac tttccagcta cactaaaaat
    14761 agataatgtc gcacctattt gataactaca agaactacca aagcttatta aactcataca
    14821 ctgtgtcttc gtgcaggtat ggcattggtg tgctgcttgt gaaatagctg ctgcattaca
    14881 tccaaatcct tctaatagtg gcacgatatc attaccttgt aatccgatcc ataacattga
    14941 cggttcaatt gcccatatca tccatgattt gagtcctgtt tgatcaatta ttgcagttga
    15001 taaactaatt aatattacaa ccggcaatgc ccatacgagc gaatatgttc caagtgatag
    15061 cacaccataa tcaccaaata atatatgctg taatatatcg tgattgagca atgttgtttg
    15121 agttaaatat tctatccatg ctgatacata ctgattttga aaccaatctg aaaattgata
    15181 agcgatacca attggcaatg caaacattaa aaatatcaac aaccaggcta aaaatgtcgc
    15241 acttatttta ggtcttaaat gtcgtcgctc atgttgaaat agtgtcaatt gttctatcac
    15301 atgttcactc gctataggcg aagaaactac actttctgcc aatgaaatat ctaatttctg
    15361 ttttatgatt aataatctta atttcgcttt ttctgttgcg gtaagtttat ccgcatggct
    15421 aataaccaaa taatgaggtt catgaggtat gggcgtataa tgcccagttc tcatatcata
    15481 tttcacttct aacatgtgca tgacacttca caacatgaat aatcatctaa atacatttga
    15541 tttttttggt aattttcaat gacctcttgt ttagctggta agccttcccg ctgtgttaaa
    15601 agatgggcaa gtactgcaaa tcgcgctctg aatttataga tatagcataa tttggcatta
    15661 tcattttcaa ctgttgcacc aatcataaaa atattcggat aacgtgtcga ttcatcatgt
    15721 gttgttaatt taatatcttg atttgttgtc acaaataatt gttgaacgat tggatttttt
    15781 gttgcatcaa agccagttgc tagtattggt tcatgaggcg tatgcacact ttgtccgcta
    15841 tcaaaactga tatgatactg tccattgtta aaatcaatat ctttaactgt ataatgtaca
    15901 ttcatttcga tgcgagcacc ttgcttaatg acattaccta gtcgctgacg tgtataaggt
    15961 gacaatctaa cactaggatc agcatccgga tcatttaaac cggttgtgct agtataaagt
    16021 gcgatgtcag agccattttt tgcaagttga tatgcagcat caaagccact ttcattacct
    16081 ccgataacca catattgccc cttattaaag ttatcaaagt cttcaatttc actataatga
    16141 ataccatatt taaatggctt tttagggaaa ttataatcac ctgttgcgac aaagatataa
    16201 tccgcgtgat atgtctctgt tgtcgttgca atcgtataat atgcatcatc tgcagatata
    16261 tttgtgacaa ctgtattttc aaagatattc agctcgtaat ggttggcaac cacttgtaaa
    16321 tattcagcat atgtttctcc ggaaatatgt tcttcattaa atgtaaatgc tggtgaagta
    16381 tccatggaaa ttgcattcat atcaggcatg ccaaatccat tagacgtaaa tgatggcgtg
    16441 atcgtacggg tcgatttcgg ccaatgttta aatgaatgtc ctactgttcc tttttctaaa
    16501 ataatgacat ctgttatacc gaaatctttt aaggtaatgg ccatacctat acctgcagca
    16561 ccggcaccga taatagccac tttatgatgt tgcatattaa cactccctca atgatctatt
    16621 tcaagttatt gtttttgact ataaatcgta ataattacga tttataaagt atagacctat
    16681 ccttaagaaa tttcaatatt aaaagaaaaa cttccgttat tgtagagaat tcatctacat
    16741 ttcggaagtt ggtatgtgtc ttttttatta aatttaagga tgctacggtt agttcaaatg
    16801 attatgaatg acatttaatg gtatcatcca gcctcagtac ttggagataa cattttggcg
    16861 gtgagacata aatcatattt ttacaaaact tatttcgtcg tctccccaat aaagataaag
    16921 tatttcttta ttgtggtgta acttctccac gttgtatttc actatccaaa ttttgtttca
    16981 tattttcaaa caactcttga aatctaacct catcttgatc agaataacct ttaaaaataa
    17041 ctgaatctat attttcttcg ttaaaataat aaatttcatc ttccacaaca ccaatcggat
    17101 atttacatgc agaatagtca aacatatact tttgattttc aatttctaca attggtcctc
    17161 tattaataat cattaacttt tgcgaacctt cttttaaata aataatgctt cctattgttt
    17221 ccatttattt tcctcctttg tcaccgtctc aatttcaaca ttccttcgca tttccaagtc
    17281 aatcatctct tcttccgctt tacgaatgcc atgttgaatt tgctgttgct gctcgtataa
    17341 cgcttccgtt gaacgtcgtt tcgcaaaatg aaattcatcg cggaactgtt ccatacgact
    17401 agccatattg ctactatgat aacggtcacc taaatgttga tataattgtt ctatcgaatg
    17461 attaagctca tcactttcgt ctatcaaagc tttaacctct cgcttttcat catataagtc
    17521 atccatttgc ttatgtttcg ctgtgatcat tttattgatt ccatcaagtt tattcgacat
    17581 catttcacca cggtttaatt tttaccaatt tcattaccaa tcattacgtc tgtttgatta
    17641 aattcttcgc caactttttg tatactatcg ctcgcatttc ttattgcttc agcaacagat
    17701 tgattcttac taatcaaatc gtctatcaca ctatgagcat tattatttcc acttactgtt
    17761 gtactttcat ctttatccgg tttcgacaca ttattacatg tttcaacggc acttttaata
    17821 gatgatgatg cactattagt cttgaaaata tcactttgaa ctgtactcat tatttacacc
    17881 tcttcccatt
//
LOCUS       DANBWH010000036        17119 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.037, whole genome shotgun sequence.
ACCESSION   DANBWH010000036 DANBWH010000000
VERSION     DANBWH010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17119
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.037"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            106..987
                     /gene="rbsU"
                     /locus_tag="PPY92_000840"
     CDS             106..987
                     /gene="rbsU"
                     /locus_tag="PPY92_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose transporter RbsU"
                     /protein_id="HDJ2624859.1"
                     /translation="MSIVALLIGLGPLIGWGFFPTVASKFGGKPVHQIIGATVGTLIF
                     AIILAVVTSSGFPTGTNLLFALLSGAGWGFGQIITFKAFELIGSSRAMPVTTAFQLLG
                     ASLWGVFALGNWPGIGHKIIGFTALVVILIGARMTVWSERKEASNAKNLRRAVVLLLI
                     GEFGYWLYSAAPQATSIDGLTAFLPQAMGMVIVAVIYGFMNMKSENPFRNKITWLQII
                     SGFFFAFGALTYLISAQPNMNGLATGFILSQTSVVLATLTGIYFLKQHKTSKEMVITI
                     IGLVLILVAASVTVFIK"
     gene            1002..1406
                     /gene="rbsD"
                     /locus_tag="PPY92_000841"
     CDS             1002..1406
                     /gene="rbsD"
                     /locus_tag="PPY92_000841"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDJ2624860.1"
                     /translation="MKKSAVLNEHISKAIATIGHFDLLTINDAGMPIPNDHRRIDLAV
                     TKNLPRFIDVLATVLEEMEIQKIYLAEEIKEHNPTQLQQIKQLISSEIEIIFIPHEEM
                     KSNLAHPLNKGNIRTGETTPYSNIALESNVTF"
     gene            1434..2348
                     /gene="rbsK"
                     /locus_tag="PPY92_000842"
     CDS             1434..2348
                     /gene="rbsK"
                     /locus_tag="PPY92_000842"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDJ2624861.1"
                     /translation="MTNKVVILGSTNVDQFLTVERYAQPGETLHVEEAQKAFGGGKGA
                     NQAIATARMQADTTFITKIGTDGVADFILEDFKAAHIDTSYIIKTTEAKTGQAFITVN
                     AEGQNTIYVYGGANMTMTPEDVINAKDAIINADFVVAQLEVPIPAIISAFEIAKAHGV
                     TTVLNPAPAKALPNELLSLIDIIVPNETEAELLSGIKVTNEQSMKDNANYFLSLGIKT
                     VLITLGKQGTYFATKNQSQHIEAYKVNAIDTTAAGDTFIGAFVSRLNKSQDNLADAID
                     FGNKASSLTVQKHGAQASIPLLEEVNQV"
     gene            complement(2446..2580)
                     /locus_tag="PPY92_000843"
     CDS             complement(2446..2580)
                     /locus_tag="PPY92_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624862.1"
                     /translation="MIIIIINIAYLYAIIWKLKRLQKIVTSFITKHESTSIGLVENYF
                     "
     gene            2599..3360
                     /locus_tag="PPY92_000844"
     CDS             2599..3360
                     /locus_tag="PPY92_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDJ2624863.1"
                     /translation="MSKEAGHTFLAKLGKTRLRPGGKEATDWLIQQGAFSQDKQVLEV
                     ACNMCTTSIYLAHTYGCHIQGVDINKKALEKAQENISAAGLESYIQVQQANAVKLPFD
                     DNQFDIVLNEAMLTMLPIAIKEKALREYYRVLKPGGILLTHDIVIVNESHATHVVKSL
                     SAAINVNVSPQTKLGWLDLYHQAGFNHVHYHTGPMSLMTPKGLIYDEGIVGTIKIIKN
                     ALKKENRPMFCKMFKTMTKLRKDMNYITFVAKKED"
     gene            complement(3847..5283)
                     /locus_tag="PPY92_000845"
     CDS             complement(3847..5283)
                     /locus_tag="PPY92_000845"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDJ2624864.1"
                     /translation="MTKLPQNFMWGGALAANQFEGGYEKGGKGLSVIDVMTSGAHGKA
                     RQITESIDLNHYYPNHEGIDFYHRYKEDIALFKEMGLKCLRTSIAWTRIFPNGDEDVP
                     NEEGLAFYDRIFDELIAQGIEPVVTLSHFEMPLHLAKHYGGFRNREVVDYFVHFARVV
                     FERYKDKVTYWMTFNEINNQMDTSNPIFLWTNSGVALTENDNPEEVLYQVAHHELLAS
                     ALAVRLGKEINPKFKIGTMISHVPIYPYSCHPKDMMEAQIANRLRFFFPDVQVRGYYP
                     SYAKKMLARKGYDVGWQEGDDSILQQGTVDYIGFSYYMSTAVKHDVDTTVENNIVNGG
                     LNHSVENPHIATSDWGWAIDPDGLRYTLNVLYDRYQLPLFIVENGFGAVDEVVDGHIH
                     DDYRIEYLKAHITAAIEAVDQDGVDLIGYTPWGIIDIVSFTTGEMKKRYGLIYVDRDN
                     DGHGTMERLKKDSFYWYQQVIASNGDKL"
     gene            complement(5299..6090)
                     /locus_tag="PPY92_000846"
     CDS             complement(5299..6090)
                     /locus_tag="PPY92_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDJ2624865.1"
                     /translation="MSNKYKEQAQDILTAVGGVENIVDATYDTKCITIHMQHTIPSTA
                     NEVKQIVDVTSVAENDAQLVIKLNGNVDEVYQQLQRLIKNANVEESENTDNINSQDTS
                     YTPQVKVTTPILVKAPIAGRRILLKEVRDSIFREKMVGEGLAIKAHEESKVIAPFNGL
                     ISMIVPTKHAVGIQSEDGVDIVIHIGVNTVDLEGKGFKCFVKQNDHVEAGQTLLQFDQ
                     QYIQQQGYNADVIVVISNSADLGKVELTMNEIITTEDVIFKIFKN"
     gene            6239..6943
                     /locus_tag="PPY92_000847"
     CDS             6239..6943
                     /locus_tag="PPY92_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDJ2624866.1"
                     /translation="MLKYEHIAKQLNAFIHQSNFKPGDKLPNVTQLKERYQVSKSTII
                     KALGLLEQDGLIYQAQGSGIYVRNIADANRINVFKTNGFSKSLGEHRMTSKVLVFKEM
                     ATPPKSVQDELQLNADDTVYYLERLRFVDDDVLCIEYSYYHKEIVKYLNDDIAKGSIF
                     DYLESNMKLRIGFSDIFFNVDKLTSSEASLLQLSTGEPCLRYHQTFYTMTGKPFDSSD
                     IVFHYRHAQFYIPSKK"
     gene            complement(7051..7752)
                     /gene="lrgB"
                     /locus_tag="PPY92_000848"
     CDS             complement(7051..7752)
                     /gene="lrgB"
                     /locus_tag="PPY92_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HDJ2624867.1"
                     /translation="MINHLALNTPYFGILLSVIPFFLATILFEKTNRFFLFAPLFVSM
                     VFGVAFLYLTGIPYKTYKIGGDIIYFFLEPATICFAIPLYKKREVLVKHWHRIIGGIG
                     IGTVVALLIILTFAKLAQFANDVILSMLPQAATTAIALPVSAGIGGIKELTSLAVILN
                     GVIIYALGNKFLKLFRITNPIARGLALGTSGHTLGVAPAKELGPVEESMASIALVLVG
                     VVVVAVVPVFVAIFF"
     gene            complement(7745..8188)
                     /gene="lrgA"
                     /locus_tag="PPY92_000849"
     CDS             complement(7745..8188)
                     /gene="lrgA"
                     /locus_tag="PPY92_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like murein hydrolase modulator LrgA"
                     /protein_id="HDJ2624868.1"
                     /translation="MVVKQQKDASKPAHFFHQVIVIALVLFVSKIIESFMPIPMPASV
                     IGLVLLFVLLCTGAVKLGEVEKVGTTLTNNIGLLFVPAGISVVNSLGVISQAPFLIIG
                     LIIVSTILLLICTGYVTQIIMKVTSRSKGDKVTKKIKIEEAQAHD"
     gene            complement(8301..9041)
                     /locus_tag="PPY92_000850"
     CDS             complement(8301..9041)
                     /locus_tag="PPY92_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LytR"
                     /protein_id="HDJ2624869.1"
                     /translation="MKALIIDDEPLARNELTYLLNEIGGFEEINEAENVKETLEALLI
                     NQYDIIFLDVNLMDENGIELGAKIQKMKEPPAIIFATAHDQYAVQAFELNATDYILKP
                     FGQKRIEQAVNKVRATKAKDDNNASAIANDMSANFDQSLLVEIDDKIHMLKQQNIIGI
                     GTHNGITTIHTTNHKYETTEPLNRYEKRLNPTYFIRIHRSYIINTKHIKEVQQWFNYT
                     YMVILTNGVKMQVGRSFMKDFKASIGLL"
     gene            complement(9044..10798)
                     /locus_tag="PPY92_000851"
     CDS             complement(9044..10798)
                     /locus_tag="PPY92_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDJ2624870.1"
                     /translation="MLSLTMLLLERVGLIIILAYVLMNIPYFKNLMNRRRTWKARWQL
                     CIIFSLFALMSNLTGIVIDHQHSLSGSVYFRLDDDVSLANTRVLTIGVAGLVGGPFVG
                     LFVGVISGIFRVYMGGADAQVYLISSIFIGIIAGYFGLQAQRRKRYPSIAKSAMIGIV
                     MEMIQMLSILTFSHDKAYAVDLISLIALPMIIVNSVGTAIFMSIIISTLKQEEQMKAV
                     QTHDVLQLMNQTLPYFKEGLNRESAQQIAMIIKNLMKVSAVAITSKNEILSHVGAGSD
                     HHIPTNEILTSLSKDVLKSGKLKEVHTKEEIGCSHPNCPLRAAIVIPLEMHGSIVGTL
                     KMYFTNPNDLTFVERQLAEGLANIFSSQIELGEAETQSKLLKDAEIKSLQAQVSPHFF
                     FNSINTISALVRINSEKARELLLELSYFFRANLQGSKQHTITLDKELSQVRAYLSLEQ
                     ARYPGRFNININVEDKYRDVLVPPFLIQILVENAIKHAFTNRKQGNDIDVSVIKETAT
                     HVRIIVQDNGQGISKDKMHLLGETSVESESGTGSALENLNLRLKGLFGKSAALQFEST
                     SSGTTFWCVLPYERQEEE"
     gene            complement(11043..11717)
                     /gene="scdA"
                     /locus_tag="PPY92_000852"
     CDS             complement(11043..11717)
                     /gene="scdA"
                     /locus_tag="PPY92_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein ScdA"
                     /protein_id="HDJ2624871.1"
                     /translation="MINKNDIVADIVIDYPKAADIFRSVGIDFCCGGQVSIEAASLEK
                     KNVDLNELLQRLNDVEQTNTPGSLNPKFLNVSSLIQYIQAAYHEPLREEFKNLTPYVT
                     KLSKVHGPNHPYLVELKETYDTFKSGMLEHMQKEDDVDFPKLIKYEQGEVVNDINTVI
                     DDLVSDHIATGQLLVKMSDLTSSYEPPIEACGTWRLVYQRLKALEVLTHEHVHLENHV
                     LFKKVS"
     gene            complement(11861..13582)
                     /gene="tarS"
                     /locus_tag="PPY92_000853"
     CDS             complement(11861..13582)
                     /gene="tarS"
                     /locus_tag="PPY92_000853"
                     /EC_number="2.4.1.355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(ribitol-phosphate)
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDJ2624872.1"
                     /translation="MMKFSVIVPTYNSEKYITELLNSLAKQDFPKTEFEVVVVDDCST
                     DQTLQIVEKYRNKLNLKVSQLETNSGGPGKPRNVALKQAEGEFVLFVDSDDYINKETL
                     KDAAAFIDEHHSDVLLIKMKGVNGRGVPQSMFKETAPEVTLLNSRIIYTLSPTKIYRT
                     ALLKDNDIYFPEELKSAEDQLFTMKAYLNANRISVLSDKAYYYATKREGEHMSSAYVS
                     PEDFYEVMRLIAVEILNADLEEAHKDQILAEFLNRHFSFSRTNGFSLKVKLEEQPQWI
                     NALGDFIQAVPERVDALVMSKLRPLLHYARAKDIDNYRTVEESYRQGQYYRFDIVDGK
                     LNIQFNEGEPYFEGIDIAKPKVKMTAFKFDNHKIVTELTLNEFMIGEGHYDVRLKLHS
                     RNKKHTMYVPLSVNANKQYRFNIMLEDIKAYLPKEKIWDVFLEVQIGTEVFEVRVGNQ
                     RNKYAYTAETSALIHLNNDFYRLTPYFTKDFNNISLYFTAITLTDSISMKLKGKNKII
                     LTGLDRGYVFEEGMASVVLKDDMIMGMLSQTSENEVEILLSKDIKKRDFKNIVKLNTA
                     HMTYSLK"
     gene            complement(13615..15303)
                     /locus_tag="PPY92_000854"
     CDS             complement(13615..15303)
                     /locus_tag="PPY92_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDJ2624873.1"
                     /translation="MVKSKIYIDKIYWERVQLFVEGHSENLDLEDSNFVLRNLTETRT
                     MKANDVKIDGNQFVCRFNVAILDNGYYLPEDKYLLVNEQELDYIAQLNPDVINDAYQN
                     LKPEQEEEYNELETQNGKINFLLQTYLKEFRKGGISKKTVYTVTPEISSDVNEFVLDV
                     VVTTPEVKSIYIVRKYKELRKYFRKQSFNTRQFIFKAIFNTTKFFHLKKGNTVLFTSD
                     SRPTMSGNFEYIYNEMLRQNLDKKYDIHTVFKANITDRRGIIDKFRLPYLLGKADYIF
                     VDDFHPLIYTVRFRRSQEVIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKAYV
                     SSETDIPFYAEAFGIKEKNVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKVIL
                     FAPTFRGSGHGTAHYPFFKIDFERLARYCEKNNAVVLFKMHPFVKNRLNIADKHKQYF
                     VDVSDFREVNDILFITDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEPYE
                     SFVPGKIVQSFDALMDALDNEDYEGEKVIPFLDKHFKYQDGRSSERLVRNLFGS"
     gene            complement(15325..16350)
                     /locus_tag="PPY92_000855"
     CDS             complement(15325..16350)
                     /locus_tag="PPY92_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDJ2624874.1"
                     /translation="MINQVYQLVAPRQFEVTYNNVDIYSDYVIVRPLYMSICAADQRY
                     YTGSRDENVLSQKLPMSLIHEGVGEVVFDSKGVFNKGTKVVMVPNTPTEKDDVIAENY
                     LKSSYFRSSGHDGFMQDFVLLNHDRAVPLPDDIDLSIISYTELVTVSLHAIRRFEKKS
                     ISNKNTFGIWGDGNLGYITAILLRKLYPESKIYVFGKTDYKLSHFSFVDDVFFINKIP
                     EGLTFDHAFECVGGRGSQSAINQMIDYISPEGSIALLGVSEFPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFQDVVDLYIQYPDIVDKLALLKGQEYEIATINDLTEAFEADLSTSWGK
                     TVLKWIM"
     gene            complement(16343..17059)
                     /locus_tag="PPY92_000856"
     CDS             complement(16343..17059)
                     /locus_tag="PPY92_000856"
                     /EC_number="2.7.7.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribitol-5-phosphate cytidylyltransferase"
                     /protein_id="HDJ2624875.1"
                     /translation="MKYAGILAGGIGSRMGNVPLPKQFLDLDNKPILIHTLEKFILIN
                     DFEKIIIATPQQWMTHTKDTLRKFKISDERIEVIQGGSDRNDTIMNIVKHIESTNGIN
                     DDDVIVTHDAVRPFLTHRIIKENIQAALEYGAVDTVIDAIDTIVTSKDNQTIDAIPVR
                     NEMYQGQTPQSFNINLLKESYAQLSDEQKSILSDACKIIVETNKPVRLVKGELYNIKV
                     TTPYDLKVANAIIRGGIADD"
BASE COUNT         5459 a         3207 c         2588 g         5865 t
ORIGIN      
        1 ttaaacgttt tcagttttta tgaaaacgca tgcattttgc aaataaaaat ggtacgatgg
       61 cattggtaaa acgttttact aaaaacaaat cgtgaggtgt ataacatgag cattgttgca
      121 ttacttatcg ggttaggccc cttaattggc tggggcttct tcccaacagt cgcttcaaag
      181 tttggtggta aacctgtaca tcaaattatc ggtgctactg taggtacgtt aatcttcgct
      241 attatattag ccgtagtcac atcaagtggc ttccctactg gaaccaattt gctattcgcc
      301 ttattatcag gtgcaggatg gggattcgga caaatcatta catttaaagc gttcgaatta
      361 atcggctcat ctcgtgccat gccagtcaca acagcattcc aattattagg cgcatcttta
      421 tggggtgtct ttgcattagg aaattggcca ggcattggtc ataaaatcat tggatttaca
      481 gctttagtcg ttattctaat tggtgcacgt atgacagttt ggagtgaacg caaagaagca
      541 agtaacgcca aaaatttacg tcgtgcagtg gtacttctgt taattggtga atttggatac
      601 tggttatatt cagctgcacc gcaagcaact tctattgatg gcctaactgc ctttttacct
      661 caagcaatgg gtatggtaat tgttgcagtc atttatggct ttatgaatat gaaatcagag
      721 aatccattcc gtaataaaat tacgtggtta caaattattt caggtttctt ctttgcattt
      781 ggtgccttaa catatcttat ttcagcacaa cctaatatga atggtttagc aactggattt
      841 attctttctc aaacatccgt tgtgcttgct acattaactg gtatttattt cttaaaacaa
      901 cataaaactt caaaagaaat ggttattaca attatcggct tagtactcat tttagtagcc
      961 gcttctgtta cagtatttat aaaataagga gtgtagatgt catgaaaaaa tcagctgttt
     1021 taaatgaaca tatttcaaaa gcaatcgcga caattggtca ttttgattta ttaacgatta
     1081 atgacgctgg catgccaatt ccaaatgatc atcgccgtat cgacctagct gtcacaaaaa
     1141 acttaccacg ctttattgat gtcttagcta cagtgttaga agaaatggaa atccaaaaaa
     1201 tatacttagc agaagaaata aaagaacata accctacaca attgcaacaa attaaacaat
     1261 tgatttcatc ggaaatcgaa atcattttca ttcctcacga agaaatgaaa agtaacttag
     1321 ctcacccatt aaataaaggt aatattcgta ctggtgaaac aacgccctac tctaatattg
     1381 cattagaatc gaatgttact ttttaaaaat tataacttga aaggagcata cacatgacca
     1441 acaaagttgt tattttaggt tcaacgaatg tcgatcaatt tttaacagtt gaaagatatg
     1501 cacaaccagg cgaaacatta catgttgaag aagcacaaaa agcattcggc ggaggtaaag
     1561 gtgccaacca ggctattgcc actgcacgca tgcaagcaga cacaacattt attactaaaa
     1621 ttggcactga tggcgttgct gatttcatct tagaagattt taaagcggct catattgata
     1681 catcatatat tatcaaaaca actgaagcaa aaacgggcca agcctttatc actgtgaatg
     1741 cagaaggaca aaacaccatc tatgtttatg gtggtgcgaa tatgacgatg acacctgaag
     1801 atgttattaa cgcaaaagac gctataatca atgcagactt tgtcgttgca caattagaag
     1861 tacccatccc ggctattata tctgcatttg aaattgccaa ggcacatggt gtgacgacag
     1921 tattaaatcc tgcaccagcg aaagcattac ctaatgaatt attatcatta atcgatatta
     1981 ttgtgccaaa cgaaacagaa gccgaattgt tatctgggat taaagtaact aatgaacaat
     2041 ctatgaaaga caatgccaat tactttttat ctttaggcat taagactgtt ttgattacgc
     2101 taggtaagca aggtacatat tttgctacta aaaatcaaag ccaacacatc gaagcttata
     2161 aagtaaatgc gattgataca actgctgcag gcgacacatt tattggtgca tttgtcagtc
     2221 gcttaaacaa gtcgcaagat aacttagcag atgctattga ttttggtaat aaagcgagct
     2281 cactcactgt acaaaaacac ggcgcgcaag catctattcc tctactagaa gaagtaaatc
     2341 aagtttaaat gaatcaaaca cagctatgat atgaaggttt agcatataac atgcaacatt
     2401 cgtatatcat ggctgtgctt ttttatcttt ataaaacatc atctattaga aataattttc
     2461 cactaaacct atgcttgttg actcatgttt agttataaat gaagtgacaa ttttttgtaa
     2521 tctttttaac ttccaaatta ttgcatataa atatgctata ttaatgataa taattatcaa
     2581 ttaaaaggag gttatgctat gtctaaagaa gctggtcata catttttagc taaattagga
     2641 aaaactcgtc tacgccccgg tggtaaagaa gcaacagatt ggttaataca acaaggggca
     2701 ttttcacaag ataaacaagt gttagaagtg gcatgtaata tgtgcacaac atctatttat
     2761 ctagctcata catatggctg tcacattcaa ggcgttgata taaataagaa agcattagaa
     2821 aaagcacagg aaaatatttc agcagcaggt cttgaatcat atattcaagt tcaacaagcg
     2881 aatgctgtta aattgccctt tgatgacaat caattcgata tcgttttaaa tgaagcaatg
     2941 ttaacaatgt tacccatcgc cataaaggaa aaagcattac gcgagtacta ccgagtctta
     3001 aagcctgggg gtatcttgtt aacacatgat attgtcatcg ttaatgaatc acatgccaca
     3061 catgttgtta aatcattatc tgcagcaatt aatgtcaatg tctcaccgca gacgaaactt
     3121 ggctggttag atttatatca tcaagctggt tttaatcatg tgcattatca tactggtcca
     3181 atgagtttaa tgacaccaaa aggtttaatt tatgacgaag gtattgttgg aactataaag
     3241 attatcaaaa atgctttaaa aaaagaaaat cgaccaatgt tttgtaaaat gtttaaaacg
     3301 atgactaaat tgcgtaaaga tatgaattat attacttttg tcgctaaaaa agaggactaa
     3361 atataatgcc actaactgta ctttgtatct atgtttgact gctactttaa tttctttgtg
     3421 acactaatca tctacttaac aatatcgtta tcgttgatta gtaagtcatc aatttgggtt
     3481 aaagactttc ataaacactc aaacattaac actatactta gttagtggca ttattttttt
     3541 aaaaatttta acatcacggg attggaacag aaatgatatt ttcgcaaaat ttattcctgt
     3601 ccttccccaa cttgcattac ctgtagaatt tcttttcgaa attctctgtg ttggggcccc
     3661 gccaacttgc attgcctgta gaatttcttt tcgaatttct ctgtgttggg gccccgccaa
     3721 cttgcattac ctgtagaatt tcttttcgaa attctttgtg ttgggggccc gccattataa
     3781 cactgtaggt cctactatat taatttatgt ctcgggctct aaccctaaaa tacttataat
     3841 atacctttat aatttatctc catttgatgc tatcacttgt tgataccaat agaacgaatc
     3901 ttttttcaag cgttccatcg tgccatgacc atcattatct cgatcaacat atattaaacc
     3961 atagcgtttc ttcatttcac cggttgtaaa tgaaacaata tcaatgattc cccacggtgt
     4021 ataaccgatt aaatctacac catcttgatc aactgcttct atcgctgctg taatatgtgc
     4081 ttttaaatat tcaatgcgat aatcatcatg aatatgtcca tctaccactt catcaactgc
     4141 accaaaacca ttttccacaa taaaaagtgg taactgataa cgatcatata acacattcaa
     4201 tgtatatctt aagccatctg gatcaatcgc ccaaccccaa tcactcgttg cgatatgcgg
     4261 attctccaca gaatgattca aaccaccgtt gacgatgttg ttttcaactg tagtatcaac
     4321 atcatgtttt acagccgtag acatgtaata actaaagcca atataatcaa ccgtgccctg
     4381 ctgtaaaata ctgtcgtccc cttcttgcca tccaacatca tatccttttc gtgccaacat
     4441 ttttttagca tagcttggat aataacctct cacttggaca tccgggaaaa agaaacgtaa
     4501 gcgattcgca atttgtgctt ccatcatatc tttcggatga cacgaatatg gataaatggg
     4561 tacatgtgaa atcattgttc caatcttaaa cttcggatta atctctttac caagacgaac
     4621 tgctaaagca ctggctaaaa gttcatgatg tgctacttga tacaagactt cttcaggatt
     4681 atcattttct gtcaatgcta ccccagaatt cgtccataaa aagataggat ttgatgtgtc
     4741 catctgatta ttaatttcat taaacgtcat ccaatatgta actttatctt tatatctttc
     4801 aaatacaaca cgcgcaaaat gcacaaaata atcgacaact tctctatttc taaatccacc
     4861 ataatgtttc gctaaatgaa gtggcatctc aaaatgtgat aacgtcacaa caggttcaat
     4921 accttgtgca attaattcat caaagatacg atcataaaag gcaagtcctt cttcatttgg
     4981 cacatcttca tccccattcg gaaagatacg tgtccacgca atcgacgtac gtaaacattt
     5041 caatcccatt tctttaaaca aggcaatatc ttccttataa cgatgataaa aatcaatacc
     5101 ttcatgattt ggataatagt gattgaggtc tatagattct gtaatctgac gtgctttgcc
     5161 atgtgcacca ctcgtcataa catcaattac acttaaccct ttaccacctt tttcatatcc
     5221 accttcaaat tgatttgcgg caagagcgcc accccacata aaattttgcg gtaattttgt
     5281 cattattaca acacactcct agtttttaaa tattttaaaa ataacatctt cagtcgtaat
     5341 gatttcattc attgtcagtt ctacttttcc taaatcggca gagttgctaa taacgacaat
     5401 aacgtcagca ttgtagcctt gttgttgtat atattgctgg tcgaattgca acaacgtttg
     5461 ccctgcttca acatgatcat tttgctttac aaagcacttg aaccctttac cttccaagtc
     5521 aactgtattc acgccaatat gaatgactat gtccacaccg tcttctgatt gaataccaac
     5581 tgcatgctta gttggtacaa tcatagatat taaaccattg aacggtgcga ttactttgga
     5641 ttcttcatga gctttgattg ctaagccttc acctaccatt ttctctctaa aaattgaatc
     5701 tcttacttct ttaagtaaaa tacgacgacc agcgattggt gctttcacta aaattggtgt
     5761 tgttactttt acttgaggtg tataacttgt atcttggcta ttaatattgt cagtattctc
     5821 actctcttcg acattagcat tcttaattaa tcgctgtaat tgctgataca cttcatcgac
     5881 atttccattt aattttatga ctaactgcgc atcattttct gctacagatg tcacatcaac
     5941 tatttgtttc acttcatttg ctgtagaagg aattgtatgt tgcatatgaa ttgtaatgca
     6001 cttcgtatca tacgttgcat caacaatgtt ttcgacacca cctacagctg taagaatgtc
     6061 ttgggcttgt tctttatatt tattgctcat aaatattacc ccctcaactt gatagtagaa
     6121 tgaaaatgta tttacttacc tccaattata attgtaccgg ttcaatttgt aaacgccgat
     6181 acaattataa tattttgtgc tataataata acagacaaag tgaaacgagg acagaatatt
     6241 gttaaagtat gaacatattg ctaagcaact taatgcgttt atacatcaat ctaatttcaa
     6301 acccggtgat aaattgccaa acgtgacgca attaaaagaa cgttatcaag taagtaagag
     6361 tactatcatt aaagcattag gcttattgga acaagatggt ttgatctatc aagcacaagg
     6421 cagtggtatt tatgtgagaa atattgcaga tgccaatcgt atcaacgtct ttaagactaa
     6481 tggtttctct aaaagtttag gtgaacatcg aatgacaagt aaggtacttg tttttaagga
     6541 gatggcaacg ccacctaaat ctgtacaaga tgaactccaa ttaaatgcag atgataccgt
     6601 ctactattta gagcgattaa gattcgtgga cgatgatgtt ttatgtatcg aatattctta
     6661 ttatcataaa gaaatcgtga aatatttaaa tgatgatatt gctaagggct ctatcttcga
     6721 ttatttagaa tcaaacatga aacttcgtat tggtttttca gatattttct ttaatgtaga
     6781 taaactcact tcaagtgaag cttcattact acaattgtct acaggtgaac catgtttacg
     6841 ttaccaccag actttttata caatgactgg caaacccttt gattcgtctg acatcgtatt
     6901 tcattatcgt catgcacagt tttatattcc tagtaaaaag taataaatac ataaaaacgt
     6961 ctatatccca gttataaact ggagtataga cgttttttta cgataataac aatggctcaa
     7021 attgctatta tcttgcttag gtttttcgtt ttagaagaat attgctacaa agacaggcac
     7081 aactgctaca acaactacac caactaacac taaagctata cttgccattg attcttctac
     7141 aggtcctaat tctttggctg gtgctacacc taatgtgtga ccacttgttc caagtgctaa
     7201 tcctcgggca atagggttag taattcggaa aagcttcaag aatttattac ctagggcata
     7261 aataatgaca ccatttaaaa taactgctaa tgatgttaat tcttttatac caccgatacc
     7321 agctgatact ggtaacgcaa tcgctgtagt tgctgcttga ggtaacattg ataaaataac
     7381 atcattggca aattgtgcta acttcgcaaa agttaaaata attaataacg ctacaactgt
     7441 accgatacca atacctccta tgatacgatg ccaatgttta acaagcactt cacgcttttt
     7501 atataacgga atcgcaaaac agattgttgc cggttctaag aagaagtaaa taatgtctcc
     7561 acctattttg taagtcttat acggaatgcc tgttaaatag aggaaggcca caccaaatac
     7621 catactgaca aatagcggtg cgaataagaa gaaacgatta gttttttcaa ataatatggt
     7681 cgctaagaaa aatggtataa cggataacag tattccgaag taaggtgtgt ttagtgctaa
     7741 gtggttaatc atgagcttgt gcctcctcta ttttgatctt ttttgtgact ttgtcacctt
     7801 tagatctcga agtaactttc ataataattt gtgtgacata gccagtacaa ataagtaata
     7861 gtattgttga gacgattatt agtccaatga ttaaaaatgg tgcttggcta atgacaccta
     7921 aagagttaac aactgagata ccggctggta cgaagagtaa gccaatgtta tttgttagtg
     7981 tcgttcctac tttttcgact tcgcctaact taacagcacc agtacataat aatacaaata
     8041 ataatactaa accgattact gatgcaggca taggaattgg cataaatgat tcaattattt
     8101 tcgatacaaa gagtactaaa gcaattacaa tgacttggtg aaaaaagtgt gctggttttg
     8161 atgcgtcttt ttgttgtttc acgaccattg cctcctacgt ttgatttaac taaagtatag
     8221 atggctcact tcgatttgcg tgatttttag tccgaaatac aaaatatcat aggtaaaatg
     8281 cataaaaaaa aggattactg ttaaagtaat cctatcgacg ctttaaaatc tttcataaat
     8341 gaacgtccaa cttgcatctt gacaccattt gtcaatatta ccatataagt gtagttaaac
     8401 cattgttgca cttctttaat gtgtttcgtg ttaataatat atgaacgatg aatacgtata
     8461 aaataagtgg gattcaatcg tttttcataa cgattcaatg gctctgttgt ttcgtattta
     8521 tgattcgttg tatgtatggt tgtaatacca ttatgtgtgc caatcccaat aatattttgt
     8581 tgctttaaca tgtgaatttt atcgtcaatt tcaacaagta agctttgatc aaaattcgcc
     8641 gacatatcat tcgcaattgc acttgcgtta ttatcatctt tggctttagt cgcacgcact
     8701 ttattgactg cttgttcaat acgtttttga ccaaacggtt tcaaaatata gtctgtcgca
     8761 tttaattcaa atgcctgtac tgcgtattgg tcatgtgcag ttgcaaaaat aatcgcaggt
     8821 ggctctttca tcttttgaat cttagctcct aattcgatcc cattttcatc cattaaattg
     8881 acatctaaaa atataatgtc atattgattg atcagtagtg cttccaatgt ttcttttaca
     8941 ttttctgcct cattaatttc ttcaaaacca ccaatttcat ttaataaata tgttaattca
     9001 ttacgtgcta atggctcatc atctatgatt aatgctttca tatttattcc tcctcttgtc
     9061 tttcataagg aagtacacac caaaaagtgg taccgctcga tgtcgattca aattgtaatg
     9121 ctgcggattt tccaaataat ccttttaggc gtaagtttaa attttctaaa gcactaccag
     9181 ttccagattc tgattctaca gatgtttctc ccaacaaatg cattttatct ttagaaatac
     9241 cctgaccatt atcttgtaca ataatacgta catgtgttgc agtttcttta atcactgaca
     9301 cgtcaatatc gttaccttgc tttcgattcg taaacgcatg tttgatggca ttttcaacta
     9361 aaatttgaat taaaaatggt ggtacaagca catcgcgata tttgtcttca acattaatat
     9421 tgatattaaa tcttcctgga taacgtgctt gttcgagtga taagtatgca cgcacttgac
     9481 ttaactcttt atctaaagta atcgtatgtt gctttgagcc ttgtaaattc gctctgaaaa
     9541 aataactcaa ttctaatagt aactctcgtg ccttttcgct atttattcta actaaagctg
     9601 aaatcgtgtt aattgaattg aagaaaaaat gtggactcac ttgtgcctgt aatgacttaa
     9661 tctcagcatc tttcaataac ttactttgcg tttcggcttc accaagttca atttggctac
     9721 taaaaatatt tgccaatcct tctgcaagtt gacgttccac aaaagttaaa tcattagggt
     9781 ttgtaaaata catcttcaat gtaccgacga tagaaccatg catctcaagt ggtatcacga
     9841 tagctgctct aagcgggcaa ttcggatgac tacaaccaat ctcttcttta gtatgcactt
     9901 ctttcaactt tcctgatttc aatacatctt tagacagact tgttaatatt tcatttgttg
     9961 gtatgtgatg atcactacct gcacctacat gcgataagat ttcatttttg cttgtaattg
    10021 ctacggcaga tactttcatt aaatttttaa taatcatcgc aatttgctgt gccgattctc
    10081 tattcaatcc ttctttaaaa tacggcaatg tctggttcat caattgcagt acgtcatgtg
    10141 tttgaacagc cttcatttgc tcttcttgct ttaatgttga aatgataata gacataaaaa
    10201 tcgccgtacc aacgctatta acaataatca ttggtagtgc aattaatgat atgaggtcaa
    10261 ccgcatatgc tttgtcgtgg gaaaatgtta aaatgctcaa catttgaatc atttccataa
    10321 caattccaat catggcactt ttcgcaatac tcgggtaacg cttgcgtctt tgagcttgta
    10381 agccaaaata accagcaatt ataccgataa atatagatga gataagataa acttgtgcat
    10441 ccgccccacc catatacact ctgaaaatac ctgaaataac gccaacaaat agacctacaa
    10501 aagggccacc aactaatcct gcgacaccta tcgttaatac acgtgtgtta gctaaagata
    10561 catcatcatc taaacggaag tacacacttc ctgacaaact atgttgatga tcgatgacga
    10621 taccagttaa attagacatt aaggcaaaca aactgaaaat aatacataat tgccaacgtg
    10681 ctttccatgt acgtcgacga ttcattaagt ttttaaaata tggaatattc atcaacacat
    10741 aggccaaaat aataattaaa cctacacgct caagtaataa cattgttagc gatagcacga
    10801 tagcacctca gtgaattata attatgttta ttttatcata atagtgcatg taaaattcat
    10861 gaataaatta ttaaaaaaac acgctactat ttaattatct ataccattaa attttcatat
    10921 caatttttat gctaagttga ccatttttct tttcaaataa tgctgttcat atcactaaat
    10981 ttctgttaag tatagcgaaa aactgctgac attactttgc caacagtttc taatcgcgtt
    11041 atttatgata cttttttaaa taaaacatga ttctctaaat gaacatgctc atgtgttaac
    11101 acttcaagtg cttttaatct ctgataaacg agtcgccacg taccacatgc ctctatcggt
    11161 ggttcatagc tagatgttaa atcgctcatt ttcactaaca attgtcccgt tgcaatgtga
    11221 tcagatacta aatcatcaat cactgtatta atatcgttta ctacttcacc ttgttcatat
    11281 ttaattagtt ttggaaaatc aacatcatct tctttttgca tatgctctaa catgccactt
    11341 ttaaatgtat catatgtttc ttttaactcg actaaatatg gatggttagg tccatgtact
    11401 ttcgataatt tcgtcacata aggtgttaaa tttttaaatt cttctctaag aggttcatga
    11461 tatgctgctt gaatatattg aataagtgac gaaacattta aaaatttagg gttaagcgaa
    11521 cctggtgtat tcgtttgttc aacgtcattg agacgctgta ataattcgtt caaatctaca
    11581 tttttctttt ctaaggatgc tgcttctata cttacttgtc caccgcaaca aaagtctata
    11641 cctacgcttc taaaaatatc tgctgctttc ggataatcta ttactatatc tgctactatg
    11701 tcatttttat ttatcattat agacgacctc ctcattttca gtgtatacaa tttaatgtat
    11761 atgaaaatga aaatcaggga aatgtcataa tcatttcata aatagcaaaa taaaaccctc
    11821 aaattatgtt agttcacata gatttgaggg catttattat ttattttagc gagtaagtca
    11881 tatgtgcagt gtttaactta acaatatttt tgaagtctcg cttcttaata tctttactaa
    11941 gtaagatttc cacttcgttt tctgacgttt ggcttaacat tcccataatc atgtcgtctt
    12001 ttagcacgac actagccata ccttcttcaa atacataacc acgatccaga ccagttaaaa
    12061 tgattttgtt tttacctttt aacttcattg agattgaatc cgttaatgta atagctgtaa
    12121 agtataacga aatgttatta aagtcttttg tgaaatacgg tgttaatcta taaaaatcat
    12181 tatttaaatg aattaatgca cttgtttctg cagtatatgc atatttatta cgttgattac
    12241 caacacgcac ttcaaatact tccgtaccta tttggacttc taagaaaaca tcccaaattt
    12301 tttctttagg taaatacgct ttaatatctt ctaacataat gttaaaacga tattgtttat
    12361 tcgcattgac acttaaaggt acatacattg tgtgcttctt gtttcgtgaa tgtaatttaa
    12421 gtctgacatc ataatgtcct tcgccaatca taaattcatt taacgttagc tctgtaacaa
    12481 ttttatgatt atcaaattta aatgctgtca ttttcacttt tggcttagcg atatcaatgc
    12541 cttcaaagta tggttcgcct tcattgaatt gaatgtttaa tttaccatct acaatatcaa
    12601 aacggtagta ttgaccttga cggtaacttt cttctacagt tctatagttg tctatatctt
    12661 tcgctctcgc gtagtgcaac aatggtcgta atttactcat caccaatgca tctacacgtt
    12721 ctggaactgc ttgtataaag tctcctagag cattaatcca ttgtggttgc tcttcaagtt
    12781 taactttaag tgagaagcca ttcgtacgag aaaaactaaa atgacgattt aaaaattctg
    12841 ctaagatttg atctttatga gcttcttcta aatctgcatt taatatttct acagcaatca
    12901 atctcatgac ttcgtaaaag tcttcaggtg aaacatacgc actactcata tgttcacctt
    12961 cacgctttgt agcataataa tacgctttat cacttaacac actgattcga tttgcattca
    13021 aatatgcttt cattgtaaat aattgatctt ctgcactctt taattcttct ggaaaataaa
    13081 tgtcattatc ttttagtaat gctgttctat agattttagt cgggcttaaa gtatagataa
    13141 ttcttgaatt taacaaagta acttcaggtg ctgtttcttt aaacatagat tgtggtacac
    13201 cacgaccatt aacacctttc attttaatca ataagacatc tgagtgatgt tcatcaataa
    13261 atgctgctgc atcctttaaa gtctctttgt ttatatagtc atcggagtcc acaaataata
    13321 caaattcacc ttctgcttgt tttaacgcca catttctagg tttacctgga ccaccagaat
    13381 ttgtttcgag ttgacttact ttcaagttca atttattgcg atacttttca actatttgta
    13441 acgtttgatc tgttgaacag tcatcaacta caaccacttc aaattcagtt ttcggaaaat
    13501 cttgtttcgc aaggctatta agtaattctg ttatatactt ttctgaattg tatgttggaa
    13561 ctattactga aaatttcatc attatacctc tcccactttg actactatat aaacttagct
    13621 accaaataaa tttctgacta aacgctcact tgatcggcca tcttgatatt taaaatgttt
    13681 atctaagaat ggaatgactt tttctccttc ataatcttca ttgtccaagg cgtccattaa
    13741 tgcgtcaaat gattgcacaa ttttacctgg aacaaatgat tcatatggtt cataaaaatc
    13801 acgcgtcgta atataatctt ctaaatcaaa tgcatagaaa atcattggct ttttaaatac
    13861 tgcatattca tatattaaag atgaatagtc actaattaat aaatctgtta tgaacagtat
    13921 atcattaact tctctaaagt cagaaacgtc aacaaaatat tgtttatgtt tgtctgcaat
    13981 attaagtcta tttttcacaa atggatgcat tttaaataat acaaccgcgt tatttttttc
    14041 gcaatatctt gctaaacgtt cgaaatcaat tttgaaaaat gggtaatgtg ctgtaccatg
    14101 accactacct ctaaatgttg gtgcgaaaag aatgactttc ttacctttaa taattggtaa
    14161 ttcatcttcc atctcttgtt tgatctgtgt cgcataagct tcatcaaata gtacatcagt
    14221 acgtggaaca cctgtaggca ctacattttt ctctttaata ccaaatgctt cagcgtagaa
    14281 tggaatatcg gtttcagatg atacataagc ttttgtatag ctacgatgat ttaatgaatc
    14341 aataaatggt ccaccctttt taccagtacg actaaagcca actgttttaa aggcaccaac
    14401 ggcatgccat acttgaataa cttcttgaga acgtctaaaa cgcactgtat aaatcaatgg
    14461 gtgaaagtca tcaacaaaga tgtagtctgc cttcccaagt aaatatggca atctaaactt
    14521 gtcgatgatg ccacgtctat ctgtaatatt cgctttaaaa acagtgtgaa tatcatactt
    14581 tttatctaaa ttttgacgta acatttcgtt atagatgtat tcaaagtttc cagacatcgt
    14641 tggtctagag tctgatgtga acaacaccgt attccctttt ttcaagtgga aaaatttcgt
    14701 cgtattaaat atcgctttaa aaataaattg tcttgtatta aatgattgtt tgcggaaata
    14761 cttacgtaat tctttatatt tacgaacgat ataaatactt ttaacttccg gagtcgttac
    14821 aacaacatca aggacaaatt cattaacatc gctagaaatt tcaggtgtaa cagtataaac
    14881 cgttttcttc gaaatgccgc cttttctaaa ttcttttagg taagtctgca ataagaaatt
    14941 gattttacca ttttgtgttt ctaattcgtt gtattcttct tcttgttctg gctttagatt
    15001 ttgatatgca tcattaatca catctgggtt taactgtgca atataatcaa gttcttgctc
    15061 attcactaat aagtacttat cttcaggtaa gtaataacca ttatctaaga tagctacatt
    15121 gaaacgacaa acgaattgat tcccatctat tttgacatca ttcgccttca ttgtacgtgt
    15181 ctcagttaaa tttcttaata caaaattact atcttctaaa tctaggtttt cactatgtcc
    15241 ttcaacgaat aactgaacac gttcccaata gattttatct atatatatct tacttttaac
    15301 caacgttaat tcatcctttt ctatttacat aatccatttt aatactgttt taccccaaga
    15361 tgtagacagg tctgcttcaa aagcttctgt aagatcatta attgttgcaa tttcatattc
    15421 ttgacctttt aacaacgcta atttatctac aatatctggg tattgaatgt ataagtctac
    15481 aacatcttgg aaatcttttg aaccacttcg actactacca atcaacgtta gtcctttttc
    15541 caatactaga cgtgtattaa cttctactgg gaactcactt acacctaaca gtgcaatgct
    15601 tccttctggt gaaatataat caatcatttg atttattgct gattggctac cacgcccacc
    15661 aacgcactca aatgcatgat caaatgttaa gccttcaggt attttattaa taaagaagac
    15721 atcatcaaca aatgagaagt gactcaattt ataatctgtt ttaccaaaga catatatttt
    15781 agactctgga tataatttac gtaataaaat ggctgtaatg taacctaagt taccatcacc
    15841 ccaaatacca aatgtatttt tatttgaaat agatttcttt tcaaaacgac gaatagcatg
    15901 caaacttact gttacaagct ctgtatatga aataatactt aaatcaatat catcaggtag
    15961 tggtacagct ctatcatgat ttagcaacac aaaatcttgc ataaacccat catgtccact
    16021 tgatctgaag tagctcgatt ttaaatagtt ttcagcaatg acatcgtctt tttctgtcgg
    16081 cgtattcggt accataacta cttttgtacc tttattaaac acacctttac tgtcaaatac
    16141 gacctcacca acaccttcat gaattaaaga cattggcaat ttctgagata agacattctc
    16201 atcacggcta ccagtataat atctttgatc ggcagcacaa attgacatat ataaaggacg
    16261 tacaatgaca tagtcactgt aaatatctac gttgttatac gtaacttcaa attgtctagg
    16321 tgcaactaat tgatatactt gattaatcat cggcaatacc acctcgaata atagcattcg
    16381 ctacttttaa atcgtaaggt gttgttactt taatgttata taactcacct tttacaagtc
    16441 gaaccggttt gtttgtttct acaataatct tacaagcatc agataaaata ctcttttgct
    16501 catcactcaa ctgtgcatag ctttctttta ataaattaat attaaacgat tgaggtgttt
    16561 gaccttggta catttcatta cgcactggaa ttgcatcaat cgtttgatta tctttagatg
    16621 taacaatcgt atctatagca tcaatcactg tatctactgc accgtactct aaagcagctt
    16681 gaatattttc tttaataata cgatgcgtta aaaatggtct aactgcatca tgtgtcacaa
    16741 tgacatcgtc atcgttaata ccatttgttg attcaatatg tttaacgata ttcataattg
    16801 tatcgttacg atcgctacca ccttgaatga cttcaattct ttcatcagaa attttgaatt
    16861 ttctaagtgt atctttcgta tgcgtcatcc attgttgtgg cgtcgcgata ataatttttt
    16921 caaaatcatt aattaaaata aatttttcta atgtatggat taaaatcggt ttgttgtcta
    16981 aatctaaaaa ttgtttaggt aaaggtacgt tacccattct tgagcctata cctccagcta
    17041 gaataccagc gtatttcata aaatacttcc tccattcaac tatatctata tttaattatt
    17101 taaatttcgt tgcattttc
//
LOCUS       DANBWH010000037        16860 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.038, whole genome shotgun sequence.
ACCESSION   DANBWH010000037 DANBWH010000000
VERSION     DANBWH010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16860
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.038"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(34..1161)
                     /locus_tag="PPY92_000857"
     CDS             complement(34..1161)
                     /locus_tag="PPY92_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDJ2624876.1"
                     /translation="MKSKYEPLFDKVELPNGVELRNRFVLAPLTHISSNDDGTISDVE
                     LPYIEKRSQDVGITINAASNVSDVGKAFPGQPSIAHDSDIEGLKRLATAMKKNGAKAL
                     VQIHHGGAQALPELIPDGDVVAPSPISLKSFGQKQEHSAREMTNEEIEQAIKDFGEAT
                     RRAIEAGFDGVEIHGANHYLIHQFVSPYYNRRNDVWANQYKFPVAVIEEVLKAKEAYG
                     NKDFIVGYRLSPEEAESPGITMEITEELVNKISHMPIDYIHVSMMDTHATTREGKYAG
                     QERLPLIHKWINGRMPLIGIGSIFTADEALDAVENVGVDLVAIGRELLLDYQFVEKIK
                     DGREDEIINYFDPEREDNHHLTPNLWHQFNEGFYPLPRKDK"
     gene            complement(1523..1900)
                     /locus_tag="PPY92_000858"
     CDS             complement(1523..1900)
                     /locus_tag="PPY92_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDJ2624877.1"
                     /translation="MNNYKIGQHIKVRVTGIQPYGAFVETPNHTEGLIHISEIMDDYV
                     HNLKKFLSEGQIVKAKILSIDDEGKLNLSLKDNDYFKNYERKKEKQSVLDEIRETEKY
                     GFQTLKERLPIWIKQSKRAIRND"
     gene            complement(2315..2908)
                     /locus_tag="PPY92_000859"
     CDS             complement(2315..2908)
                     /locus_tag="PPY92_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDJ2624878.1"
                     /translation="MANYPQLNKEVQQGEIKVVMHTNKGDMTFKLFPNIAPKTVENFV
                     THAKNGYYDGITFHRVINDFMIQGGDPTATGMGGESIYGGAFEDEFSLNAFNLYGALS
                     MANSGPNTNGSQFFIVQMKEVPQNMLSQLADGGWPQPIVDAYGEKGGTPWLDQKHTVF
                     GQIIDGETTLEDIANTKVGPQDKPLHDVVIESIDVEE"
     gene            2972..3355
                     /locus_tag="PPY92_000860"
     CDS             2972..3355
                     /locus_tag="PPY92_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase-associated lipoprotein B"
                     /protein_id="HDJ2624879.1"
                     /translation="MKLYRFSHKTGSYGVTIVEENGDDVLVKVEQVIKHPKQGDLHNP
                     TETEGVFFHERKALSHFEKRYTKRSQLRDFNVDVLRYEDSLQQAITDLETKLKAQQTK
                     HAEMSLASLARLKEDYSLQYKENFK"
     gene            3486..5891
                     /gene="mnhA1"
                     /locus_tag="PPY92_000861"
     CDS             3486..5891
                     /gene="mnhA1"
                     /locus_tag="PPY92_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit A"
                     /protein_id="HDJ2624880.1"
                     /translation="MSLLHIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIY
                     MLTLIKTTMSGNTVMKTLNWMPHFGMNFDLYLDGLGLLFSLLISGIGSLVVLYSIGYL
                     SKSEQLGNFYCYLLLFMGAMLGVVLSDNVIILYLFWELTSFSSFLLISFWRERQASIY
                     GAQKSLIITVFGGLSLLGGIILLAIPTQSFSIQYMIQHASEIQNSPFFIFAMILIMIG
                     AFTKSAQFPFYIWLPDAMEAPTPVSAYLHSATMVKAGLYLIARMTPIFAASQGWVWTV
                     TLVGLITLFWASLNATKQQDLKGILAFSTVSQLGMIMAMLGIGAISYHYQGDDSKIYA
                     AAFTAAIFHLINHATFKGALFMITGAVDHSTGTRDVKKLGGLLTIMPISFTITVITAL
                     SMAGVPPFNGFLSKESFLETTFTASQANLFSVDTLGYLFPIIGIVGSVFTFVYSIKFI
                     MHIFFGQYKPEQLPKKAHEVSILMLLSPAILATLVIVLGLFPGILTNSIIEPATSSIN
                     HTVIDDVEFHMFHGLTPAFLSTLVIYILGILLIVTFSYWVKLLQRQPGKLTFNYWYNR
                     SANVIPNYSEKMTNSYVTDYSRNNLVIIFGALILLTFVTIFSVPFNINFKDVSPIRIF
                     EVCIVILLLSAAFLILFAKSRLFSIIMLSAVGYAVSVLFIFFKAPDLALTQFVVESIS
                     TALFLLCFYHLPNLNRYNEKRSFQLTNALIAGGVGLSVIIIGLIAYGNRHFESISKFY
                     QERVYDLAHGKNMVNVILVDFRGMDTLFESSVLGIAGLAVYTMIKLRKKRQTQGNEVK
                     NHE"
     gene            5884..6312
                     /gene="mnhB1"
                     /locus_tag="PPY92_000862"
     CDS             5884..6312
                     /gene="mnhB1"
                     /locus_tag="PPY92_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit B"
                     /protein_id="HDJ2624881.1"
                     /translation="MNRQQNDLILQFAAVIIFFMVMVFGFSLFLAGHYTPGGGFVGGL
                     LFASSLVIITIAFDIETMRKIFPLDFKILIGIGLVFCIATPIASWFLGKNFFTHVTFD
                     IPLFILEPVHMTTAVFFDFGVLCAVVGTVMTIIISIGENE"
     gene            6312..6653
                     /gene="mnhC1"
                     /locus_tag="PPY92_000863"
     CDS             6312..6653
                     /gene="mnhC1"
                     /locus_tag="PPY92_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit C"
                     /protein_id="HDJ2624882.1"
                     /translation="MEIIMIFVSGILTAISVYLVLSKSLIRIVMGTTLLTHAANLFLI
                     TMGGLKHGTVPIYEANVKSYVDPIPQALILTAIVIAFATTAFFLVLAFRTYKELGTDN
                     VESMKGVPEDD"
     gene            6646..8142
                     /gene="mnhD1"
                     /locus_tag="PPY92_000864"
     CDS             6646..8142
                     /gene="mnhD1"
                     /locus_tag="PPY92_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit D"
                     /protein_id="HDJ2624883.1"
                     /translation="MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLV
                     AAIINLANVVKHGPIRVELGSWKAPYSIVFVLDIFSALLIITSIIITAIVILYSYQTI
                     GIERERYYYYFSVLFMLIGIIGAFTTGDIFNLFVFFEVFLMSSYFLLVIGSTKIQLQE
                     TIKYVLVNVVSSSFFVMGVAILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIF
                     VFATKAGVFPMFVWLPSAYYAPPIPIIAFFGALLTKVGVYAIARTLSLFFSDNVSFSH
                     YVILFLALLTIIFGCVGAVAYANIKKIILYNVMIAVGVILVGVAMMTESGMIGAIYYT
                     LHDMLVKLALFLLIGIMIKITGTADLRQFGGLIKRYPVLGWSFFIAALSLAGIPPLSG
                     FYGKFFIVQSTFERGFYLSGVIVLLSSLVVLYSVIRIFLQGFFGQPKGYDLNNKVDVK
                     YLTTIAIVAVVITVLYGLSADYLYPMVKAGAETFYNPSTYVKAVLGGK"
     gene            8144..8623
                     /gene="mnhE1"
                     /locus_tag="PPY92_000865"
     CDS             8144..8623
                     /gene="mnhE1"
                     /locus_tag="PPY92_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit E"
                     /protein_id="HDJ2624884.1"
                     /translation="MAVQLVLNFIIAVFWLFVTNSYTTNNFVLGFIFGLVLVYLLHRV
                     LPGRFYVITLYRIIKLVIIFLIELIKANFDVLKIIIKPSIKNEPGFFVYHTDLKKDWQ
                     IVLLSNLITLTPGTVVLGVSDDRTKIYIHAIDFSTKEQEVESIKTSLEKIVREVGEI"
     gene            8623..8916
                     /gene="mnhF1"
                     /locus_tag="PPY92_000866"
     CDS             8623..8916
                     /gene="mnhF1"
                     /locus_tag="PPY92_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit F"
                     /protein_id="HDJ2624885.1"
                     /translation="MNHNVIIVIALIIVVISMLAMLIRVVLGPSLADRVVALDAIGLQ
                     LMAVIALFSILLNIKYMIVVIMMIGILAFLGTAVFSKFMDKGKVIEHDQNHTD"
     gene            8894..9250
                     /gene="mnhG1"
                     /locus_tag="PPY92_000867"
     CDS             8894..9250
                     /gene="mnhG1"
                     /locus_tag="PPY92_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit G"
                     /protein_id="HDJ2624886.1"
                     /translation="MIKIILISLALIFVIIGALISALAAIGLLRLEDVYSRAHAAGKA
                     STLGAMSLLFGTFLYFIATQGFVNMQLIVAIIFVLITGPLSSHMIMKAAYNIKTPYTK
                     KTKVDEISEDLKDTKL"
     gene            9499..10653
                     /locus_tag="PPY92_000868"
     CDS             9499..10653
                     /locus_tag="PPY92_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDJ2624887.1"
                     /translation="MHWTIIGGGIQGTAIAQKLLSSGLTTDLLTIIDPHETFCQRFNS
                     YTNRIEMPYLRSPIVHHVHPQPFHLKQFAKQHQYTNAFYGPYQRPELTMFMNHIAHAS
                     KQYQLEDCLVQGLVQTLDKQEDKWHIKLEDGQIITTDCVVIAIGSTNIPFMPDILKDK
                     QNVNHIFEKEHDQVVYDKTDHIVGSGITAAHLALKLLNHDNDKKIHLWLNKDIEIHDF
                     DADPGWLGPKNMSSFLSTKSMPERNAIVQRERHKGSMPHELYLRLKKHIKNGRINVHK
                     TPITQISGGVINTENDSVPYQQIMVATGFEQDFMSQPLIKQLIQNYDAPINECNYPVI
                     SEKLEWIPNLFVAGCFADLELGPFGRNVMGGRKAAERIEQAFLKLQQYSA"
     gene            complement(10709..11083)
                     /locus_tag="PPY92_000869"
     CDS             complement(10709..11083)
                     /locus_tag="PPY92_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HDJ2624888.1"
                     /translation="MTHLLETFEMSIDHQEDGLVVISMPVTDKVKQPFGYLHGGASIA
                     LGETACSLGSANLIDTTKFIPLGLEMNANHIHSAKDGRVTATAEIIHRGKSTHVWDIK
                     IKNDKEQLITVMRGTVAIKPLK"
     gene            complement(11102..12418)
                     /locus_tag="PPY92_000870"
     CDS             complement(11102..12418)
                     /locus_tag="PPY92_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HDJ2624889.1"
                     /translation="MINAVVIAVILMIVLCLCRLNVVISLFISALVGGLISGMSIEKV
                     INVFGKNIVDGAEVALSYALLGGFAALISYSGITDYLVGKIINAIHAENSRWSRVKVK
                     VTIIIALLAMSIMSQNLIPVHIAFIPIVIPPLLSLFNDLKIDRRLIGLIIGFGLCFPY
                     VLLPYGFGQIFQQIIQSGFAKANHPIEFNMIWKAMLIPSMGYIVGLLIGLYVYRKPRE
                     YETRKISDSDNVTELKPYILIVTIVAILATFLVQTFTDSMIFGALAGVLVFFISRAYN
                     WYELDAKFVEGIKIMAYIGVVILTANGFAGVMNATGDIDELVKTLTSITGDNKLFSII
                     MMYVIGLIVTLGIGSSFATIPIIASLFIPFGASIGLDTMALIALIGTASALGDSGSPA
                     SDSTLGPTAGLNVDGQHDHIRDTCVPNFLFYNIPLMIFGTIAAMVL"
     gene            complement(12829..14304)
                     /locus_tag="PPY92_000871"
     CDS             complement(12829..14304)
                     /locus_tag="PPY92_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HDJ2624890.1"
                     /translation="MNFKLNNTLSNEINTLIIGIPEHLNQLERISFNHIDITESLERL
                     KHQHIIGSKVGKIYTTAFDVQDQTYRLITVGLGNLKARSYQDMLKIWGHLFQYIKSEH
                     IEDTYLLMDSFISKYDQLSDVLMACGIQSERATYEFDHYKSSKKAPFKTNLNLISESL
                     IELDFIHEGISIGQSINLARDFSNMPPNVLTPQTFAEDIVNHFKNTKVKVDVKDYDTL
                     VSEGFGLLQAVGKGSKHKPRLVTITYNGKDKDVAPIALVGKGITYDSGGYSIKTKNGM
                     ATMKFDMCGAANVVGIIEAASRLQLPVNIVGVLACAENMINEASMKPDDVFTALSGET
                     VEVMNTDAEGRLVLADAVYYANQYQPSVIMDFATLTGAAIVALGDDKAAAFESNSKVI
                     LNDILQISSKVDEMVFELPITATERASINHSDIADLVNHTNGQGKALFAASFVTHFSG
                     QTPHIHFDIAGPATTNKASYNGPKGPTGFMIPTIVQWLKQQ"
     gene            complement(14435..15643)
                     /locus_tag="PPY92_000872"
     CDS             complement(14435..15643)
                     /locus_tag="PPY92_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDJ2624891.1"
                     /translation="MAQDRKKVLVLGAGYAGLQTVTKLQKAISTEEAEITLINKNEYH
                     YEATWLHEASAGTLNYEDVLYPVESVLKKDKVNFVQAEVTKIDRDAKKVETNQGIYDF
                     DILVVALGFVSETFGIEGMKDHAFQIENVITARELSRHIEDKFANYAASKEKDDNDLS
                     ILVGGAGFTGVEFLGELTDRIPELCSKYGVDQNKVKITCVEAAPKMLPMFSEELVNHA
                     VSYLEDRGVEFKIATPIVACNEKGFVVEVDGEKQQLNAGTSVWAAGVRGSKLMEESFE
                     GVKRGRIVTKQDLTINGYDNIFVIGDCSAFIPAGEERPLPTTAQIAMQQGESVAKNIK
                     RILNGESTEEFEYVDRGTVCSLGSHDGVGMVFGKPIAGKKAAFMKKVIDTRAVFKIGG
                     IGLAFKKGKF"
     gene            complement(16097..16456)
                     /locus_tag="PPY92_000873"
     CDS             complement(16097..16456)
                     /locus_tag="PPY92_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HDJ2624892.1"
                     /translation="MPTVILTEAAAYEVKDMLKANEMPDGYLKIKVNGGGCTGLTYGM
                     GAEEAPGENDEVLEYFGLKVLVDKEDAPVLNGTTIDFKQSLMGGGFQIDNPNAIASCG
                     CGSSFRTAKVAGNPENC"
     gene            complement(16469..16705)
                     /locus_tag="PPY92_000874"
     CDS             complement(16469..16705)
                     /locus_tag="PPY92_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzB family protein"
                     /protein_id="HDJ2624893.1"
                     /translation="MNPIVEFCLSNMAKGGDYVFNQLENDPDVDVLEYGCLTHCGICS
                     AGLYALVNGDIVEGDSPEELLQNIYAHIKETWIF"
BASE COUNT         5319 a         3006 c         2549 g         5986 t
ORIGIN      
        1 tacacaatat cattagtggt aacagttaaa cacttattta tctttacgtg gtaatggata
       61 gaatccttca ttaaattgat gccataaatt aggagttaag tgatgattat cttctctctc
      121 tggatcaaag taattaataa tttcatcttc ccgtccatct ttaatttttt caacaaattg
      181 ataatccagt agtagctctc taccaatggc tactaagtca acaccaacat tttcaactgc
      241 atctaaagct tcgtcagctg tgaaaattga accaataccg ataagtggca tacgaccatt
      301 tatccatttg tgaattaaag gtagtctttc ttgtccagcg tatttacctt cacgtgtcgt
      361 tgcatgcgta tccatcattg aaacatgaat atagtcgatt ggcatatggc taattttatt
      421 aacgagttcc tctgtaattt ccattgtgat tcctggagac tccgcttcct ctggagataa
      481 tctgtatcca acaataaagt ctttattgcc atacgcttct ttcgctttaa gcacttcttc
      541 aatcacagcg accgggaatt tatattgatt tgcccataca tcatttcttc tattatagta
      601 tggtgataca aattgatgaa ttaagtaatg attcgcgcca tgtatttcaa caccatcaaa
      661 ccctgcttca attgcacgtc gtgttgcttc accaaaatcc ttgattgctt gttcaatctc
      721 ttcattcgtc atttctctag cactatgttc ttgtttctga ccgaaacttt ttaaagaaat
      781 tggacttggt gctacgacgt ctccatcagg tattaattca ggcaatgctt gtgcaccacc
      841 atgatgtatt tgtacgagtg ctttggcacc gtttttcttc attgctgtag ctaatcgttt
      901 tagtccttca atatcactgt catgcgcgat tgacggctgt cctggaaatg cttttccgac
      961 atcactcaca ttactcgcag cattaattgt aataccaaca tcttgtgaac gcttttcaat
     1021 ataaggaagt tctacatctg aaatagtacc atcatcattt gaagaaatat gtgttaaagg
     1081 ggctaacaca aatcgatttc tcaactctac tccatttggt aattctactt tatcaaacaa
     1141 tggttcgtat ttacttttca ttacttaatt cctccttaaa attattgagt tgactataac
     1201 atgtaatttt ttcattctcc aattatctgt ttatatagtt gcaattcgtt taatacttta
     1261 agttgtagtt ctcatttcta aaataaataa aaaaaacaaa tccacataca atattgcaaa
     1321 tcaacgattc tatctaacaa agataatgga tatgtcacct taaaccattt gtacgtgtaa
     1381 atagaatcaa tcatattacg ttatcgtaga caaaaagaga ttagtatcta ataaaattta
     1441 atagatacta atctctttta tagtctaaga aactttcatt tcagaccctt tgtatgattt
     1501 tcggtacgat ttatctgttc ctttagtcgt ttcgaattgc tcgctttgac tgttttatcc
     1561 agattggtaa gcgttcttta agtgtttgaa acccatattt ttctgtttct ctgatttcat
     1621 ctaatactga ttgtttttcc ttcttacgct cataattttt gaagtaatca ttatccttta
     1681 atgatagatt aagctttcct tcatcatcta tagacaaaat tttagcttta acaatttggc
     1741 cttctgatag aaatttcttc aaattatgaa cgtagtcatc cataatttct gatatatgaa
     1801 tcagtccttc agtatgatta ggggtctcaa caaacgcacc gtatggttga ataccagtta
     1861 cacgcacctt gatatgttgg ccaattttgt agttattcaa cttaataacc ccttatttcg
     1921 tttacttaac actattatca tagcacaaat cgaaatggtg gactaattaa aacatcaaat
     1981 catacgaata ttattttata aatgaataag aattctacaa acttggattt acaaataaaa
     2041 ttagcaacaa actcctatct aaatcaatat gatatccagc tactttgttt tcaatttatt
     2101 aactatttga atagcaattc tctttaaaaa cataaaaagc aacctcaact attatcttta
     2161 gaatacacat ctatttaaaa actgtgcttt cttgataaat atattgagat tgctcatcat
     2221 gttagtttat ttgtttcgtt ggaatatata ctaatccatt cataaattag ctttatccga
     2281 gtttaaaatg ttggtagtta attatgttta gctattattc ttcaacatca atagattcaa
     2341 ttacaacatc atgaagtggt ttgtcttgtg gtcccacttt tgtatttgca atatcttcta
     2401 aagtagtttc accatcaatg atttgaccga atactgtatg tttttgatct aaccatggtg
     2461 taccaccctt ttcgccatat gcatcaacga ttggttgagg ccagccacca tctgcaagtt
     2521 gacttaacat attttgaggt acttctttca tttgaacaat gaaaaattgt gaaccattag
     2581 tattaggtcc tgagttagcc attgataatg cgccatataa gttaaatgca tttaatgaaa
     2641 attcatcttc aaaagcaccg ccataaatac tttcgccacc cataccagta gctgttggat
     2701 cgccaccttg aatcatgaag tcattaatga cacggtggaa tgttattcca tcataataac
     2761 cattttttgc atgtgtcaca aaattttcaa ctgtttttgg tgcaatattt ggaaataatt
     2821 tgaatgtcat gtcaccttta tttgtgtgca taaccacttt aatttcacct tgttgtactt
     2881 ctttgtttaa ctgtggatag ttagccattt atattctcca ttcatgttat gatacatata
     2941 tattaacata cttttaaggg aggaaaatag catgaagcta tatcgatttt cacacaaaac
     3001 aggtagctat ggtgtcacga ttgttgaaga aaatggagac gatgtccttg ttaaagttga
     3061 acaagttatc aaacacccga aacaaggtga tttacataac cctactgaaa ctgaaggtgt
     3121 atttttccat gaacgcaaag cattaagcca ttttgaaaaa cgctatacga aacgttcaca
     3181 attacgcgac ttcaatgttg atgtattgcg ctatgaagat tcacttcaac aagcaattac
     3241 agatttagaa actaaactta aagctcagca aacaaaacat gctgaaatgt ctttagctag
     3301 tttagcacgc ttaaaagaag attattcact tcaatacaaa gaaaatttta aataaaaaat
     3361 gacgtgtttc ttcttattta aatacttctg aaaataaagc acaacaagga gtgtgtacgt
     3421 ttttcagaac tatatcattc atgtataatg aataacataa atatttttta aaggaggaca
     3481 acatagtgag tttgttacat attgcggtga tattaccgtt aatattcgca ctcattattc
     3541 ctatcctgta tagatttttt aaacgtatac atttaggatg gttcgtatta cctgtcccta
     3601 tcgtcatatt tatttatatg cttacattaa ttaaaacgac gatgtcaggt aatactgtaa
     3661 tgaaaacctt aaattggatg cctcattttg gtatgaactt tgatttatat cttgatggct
     3721 taggtttact ctttagtttg cttatttcag gaatcggcag tttagtagta ttatattcaa
     3781 tcggctactt aagtaagtca gaacaactag ggaattttta ttgttattta ttactattta
     3841 tgggtgcaat gttaggtgtc gtactttcag ataatgttat tattctatat ttattctggg
     3901 agctaacgtc attttctagt tttttactta tttcattctg gcgcgaacga caagcatcta
     3961 tatatggtgc tcaaaaatcg ctcataatca cagtatttgg tggtctatca ttattaggtg
     4021 gtatcatctt actcgctata ccaacacaat catttagcat tcagtacatg attcaacatg
     4081 cttctgaaat tcaaaatagc ccattcttca tttttgcgat gattttaatc atgattggtg
     4141 cttttacaaa atctgctcag tttccatttt atatttggct accagatgca atggaagcac
     4201 ctacaccagt cagtgcttat cttcattcag caacaatggt taaagcaggg ttatatttaa
     4261 tcgctcgaat gacaccaatc ttcgcagcat cgcaaggttg ggtatggact gtcacactcg
     4321 ttggtttaat tacgttattc tgggcttcgc taaacgcaac aaaacaacaa gacttgaaag
     4381 gtatactggc attttcaaca gtatcccaat taggtatgat tatggcgatg cttggaatcg
     4441 gtgccattag ttatcactat caaggcgatg atagcaaaat atatgctgct gcatttacgg
     4501 ctgcaatatt ccatttaatc aaccatgcaa catttaaagg tgccctattt atgattacag
     4561 gtgcagtgga tcattctact ggtacacgtg atgttaagaa attaggtggc ttactaacaa
     4621 ttatgcctat ttcatttaca atcactgtta ttacagcatt aagtatggct ggtgtaccac
     4681 cgttcaatgg tttcttatca aaagaatcat ttttagaaac aacatttaca gcaagccaag
     4741 caaatttatt cagtgttgat acattaggct acctattccc aatcattggt atagtgggca
     4801 gtgtcttcac atttgtatat tcaattaaat ttataatgca tatatttttc ggacaatata
     4861 aacctgaaca attacctaaa aaagcacacg aagtatcaat attaatgctt ttatcaccag
     4921 ctattttagc tacattagta attgtattgg gtttattccc tggcatttta actaattcga
     4981 ttattgaacc agctacatct tcgattaatc atacggtaat tgatgatgtt gaattccata
     5041 tgttccatgg attaacgcct gcctttttat caacattagt gatttatatt ttaggtatct
     5101 tactaattgt gacatttagt tattgggtga aattgttgca acgacaacct ggtaaactaa
     5161 cattcaatta ttggtacaac agaagtgcaa atgttattcc aaactactct gaaaagatga
     5221 cgaatagcta tgttaccgat tattcacgta ataatttagt tatcattttt ggtgcattaa
     5281 ttttattaac atttgtcact atattctcag tgcctttcaa cattaatttt aaagacgtta
     5341 gtccgattag aattttcgaa gtttgtatcg taattttatt attaagtgct gcctttttaa
     5401 ttcttttcgc taaatcaaga ttatttagca ttattatgtt aagtgcagtt ggatatgctg
     5461 tgtctgtatt gtttatattc tttaaagcac ccgacttagc attgacacag tttgtcgttg
     5521 aatctatttc aacagcattg ttcttattat gcttctacca cttaccaaat ttaaatcgtt
     5581 ataatgaaaa gcgctcattc cagcttacta atgctttaat tgctggtggt gttggtttgt
     5641 ccgtcattat tatcggttta attgcatacg gaaatagaca ttttgaatcg atatctaagt
     5701 tttatcaaga gcgcgtttat gatttagctc acggtaaaaa catggtaaac gtcatacttg
     5761 ttgatttccg tggtatggat actttattcg agtcatctgt gcttggtatc gcaggattag
     5821 ctgtatatac aatgattaaa ttacgtaaaa agcgtcaaac gcaaggtaac gaggtgaaaa
     5881 atcatgaata gacaacaaaa tgatctaatt ttgcaatttg ctgcagttat tatctttttc
     5941 atggtaatgg tctttggttt ttcacttttc ttagcaggac attatacacc tggtggcggt
     6001 ttcgtcggcg gattattatt cgcaagttca cttgttatca ttacgattgc atttgatata
     6061 gaaacaatgc gtaaaatttt cccattagat tttaaaatct tgataggtat cggattagtt
     6121 ttttgtattg cgacgcctat cgcaagttgg tttttaggca aaaatttctt tacgcatgtc
     6181 acatttgata taccgttatt tatattagaa cctgttcata tgacaacagc agtattcttt
     6241 gatttcggtg ttttatgtgc agtagttggt actgtcatga caattattat ttcgattgga
     6301 gagaacgaat agtggaaatt attatgattt ttgttagtgg tattctcaca gcaattagtg
     6361 tctatctcgt tttgtctaaa agtctgatac gaattgttat gggaactaca ctattaacac
     6421 atgcagcaaa cttattttta ataactatgg gcggacttaa acatggtact gttccaattt
     6481 atgaagcgaa cgtaaaaagc tatgttgatc ctatcccgca agcacttatt ttaacagcaa
     6541 tcgttatcgc ctttgcgaca acagcctttt tcttagtatt agcatttaga acatataaag
     6601 aattaggcac agataacgtt gagagtatga aaggagttcc agaagatgat tgaaagtaat
     6661 atgcttgtgt taacgctagt tattccagta attacagcca tattacttgt ctttattgga
     6721 aagcgtccta ttataaagcg ttatgtcgca ctaggcggta cattattaac cttggtcgcg
     6781 gcaattatca atttagcaaa tgttgttaaa catggaccaa tacgcgtaga gctaggttca
     6841 tggaaagcac catacagtat tgtctttgta ttagacatat tcagtgccct actaataatt
     6901 actagtatta ttattacagc tattgtgatt ttatattcat accaaacaat tggtattgaa
     6961 cgtgaaagat attattatta tttttcagta ctatttatgt taataggtat cattggtgca
     7021 ttcacaactg gtgacatttt caacctattc gtatttttcg aagtattttt aatgtcatct
     7081 tatttcttat tagtaattgg ttctacaaaa attcaactac aagaaacaat caaatatgtt
     7141 cttgttaacg ttgtatcgtc atccttcttc gtaatgggtg tagcgatttt atattcagta
     7201 gtaggaacat tgaatctagc agatataagc aataaattag ctaacctttc agcgcatgac
     7261 agtggcttag tcaatatcgt tttcatacta tttatctttg tttttgctac aaaagctggc
     7321 gtattcccaa tgtttgtttg gttacctagt gcatactatg caccaccaat tccaatcatt
     7381 gcattctttg gtgctttgtt aacaaaagtt ggtgtttatg caatcgctag aacattaagc
     7441 ctattcttta gtgataatgt aagcttttca cattatgtca ttttattctt agcactatta
     7501 acgatcatct ttggttgtgt cggtgctgtt gcatatgcca acattaaaaa aatcatccta
     7561 tacaatgtca tgattgctgt aggtgtgatc ttagtcggcg ttgcaatgat gacagagtca
     7621 ggtatgattg gggctattta ctatacattg cacgacatgt tagtaaaact tgcattattc
     7681 ttgcttatcg gaattatgat taaaattaca ggcactgctg atttaagaca atttggtggc
     7741 ttaattaaac gatatcctgt acttggttgg tcattcttta tcgcagcatt aagtttagct
     7801 ggcattcccc cactcagtgg cttttatgga aaatttttca ttgtccaatc aacatttgaa
     7861 agaggatttt atttaagtgg tgtcatcgtc ttattatcaa gtttagtcgt attatattct
     7921 gtcattcgca tattcctaca aggctttttc ggtcaaccaa aaggatatga tcttaataac
     7981 aaagtcgacg tcaaatattt aactacgatt gcaatcgttg cagttgtgat aactgtttta
     8041 tatggattgt cagcagatta cttatatcca atggtaaaag ctggtgcaga aacattctac
     8101 aatccatcaa cctacgttaa agctgtatta ggaggtaaat aacatggctg tacaattagt
     8161 attaaacttt attatcgcag tgttttggtt gtttgtaaca aatagttaca caacaaataa
     8221 ctttgtgtta ggtttcatct ttggcctagt tcttgtttac ctcttacaca gagtgttacc
     8281 aggtagattt tatgtcatca cgctatatcg tattattaaa ttagttatta ttttcttaat
     8341 cgaactgatt aaagctaatt ttgatgtttt gaaaattatt attaaacctt cgattaaaaa
     8401 tgaaccagga ttctttgttt atcacacaga tttaaaaaaa gattggcaaa ttgtcttgct
     8461 gtctaattta attacattaa cacctggtac tgtagtgctt ggtgtaagtg atgatcgtac
     8521 aaaaatttat attcatgcca tagatttttc aactaaagag caagaagtag aaagtattaa
     8581 aacatcatta gagaaaattg taagagaggt gggcgaaata taatgaatca taatgttatt
     8641 atcgttattg cattaatcat agttgtcatt tctatgttag ctatgctcat tcgcgttgtg
     8701 ctaggcccat cacttgccga tcgtgttgtc gcattagatg cgattggtct tcaattaatg
     8761 gcagttatag cattattcag tattttatta aatattaaat acatgattgt cgttattatg
     8821 atgattggta tattagcctt tttaggtact gcagtattct ctaaatttat ggacaaaggt
     8881 aaggtgattg aacatgatca aaatcatact gattagtctt gcacttatct ttgttatcat
     8941 cggtgcttta attagcgccc tagcagctat aggattattg agacttgaag atgtatattc
     9001 acgtgcacat gctgccggaa aagcatcaac attaggtgca atgtcattac tatttggtac
     9061 ttttttatat tttattgcta ctcaaggttt tgtaaatatg caattaatcg ttgcgattat
     9121 ctttgtatta attacaggtc ctctttcaag tcatatgatt atgaaagcag catataatat
     9181 taaaacgcct tatactaaaa agactaaagt cgatgaaatt tcggaagact taaaagacac
     9241 aaaattgtag attatataac aaaatcatga atataaatca acaacaaaca gcagtaagat
     9301 gattccaaat taggaatgat tttactgctg ttttcttttg acattgttac ctctttttca
     9361 atgatttttt ctttgactac atcttcgccc tatctacgta tatctcttta atttaattgc
     9421 ctttcatgtc gttatgtatt atgataataa taatcataaa tcgtaacgat tacgctttaa
     9481 aaaaagagag gttttattat gcattggaca attatcggcg gtggcataca gggaactgca
     9541 atcgcacaaa aactattatc aagcggatta acaacagacc tattaacaat cattgaccca
     9601 cacgaaactt tttgccaaag gtttaactca tatacaaatc gaatagaaat gccttattta
     9661 agatcaccga ttgtacatca cgtacatcca caaccgttcc atctaaaaca attcgctaaa
     9721 cagcaccaat atacaaatgc tttttatggt ccttatcaac gacctgaatt gacaatgttt
     9781 atgaatcata ttgcacatgc ttctaaacaa tatcaattag aggattgctt ggttcaaggt
     9841 ttagttcaaa ctttagataa acaagaagac aaatggcata tcaagttaga agatggacaa
     9901 attatcacta cagattgcgt cgttattgca ataggtagta caaatattcc gtttatgcct
     9961 gacattttaa aagacaaaca gaatgtaaat catatcttcg agaaagaaca tgatcaagta
    10021 gtatacgata agaccgatca tatcgttggt agtggcatta ctgctgcaca tcttgcactt
    10081 aaattgttaa atcatgataa cgataaaaag attcatttat ggctaaataa agatattgaa
    10141 atacatgact ttgatgctga tcctggttgg ttaggtccga aaaatatgtc ttcattttta
    10201 agtactaaaa gcatgcctga aagaaatgcc attgtacaac gtgaacgtca taaaggatca
    10261 atgcctcacg aactgtactt acgccttaaa aaacatatta aaaatggtcg tataaatgtg
    10321 cataaaacac ctatcactca aattagtggt ggtgtaatta acactgaaaa tgattctgtt
    10381 ccatatcaac agattatggt tgcaactggt tttgaacaag attttatgtc acaaccactt
    10441 attaagcaat taatacaaaa ttatgatgca cctatcaacg aatgtaatta ccctgttatt
    10501 tccgaaaaat tagaatggat accaaatcta tttgtcgcag gatgctttgc agacttagaa
    10561 ttaggaccat ttggtagaaa tgttatgggt ggccgtaaag ctgccgaacg cattgaacaa
    10621 gcatttctaa aattacaaca atatagcgca taaaaaaact gctagccgta gttatgaata
    10681 tctcataaca tttcagctag cagttctttt attttaaagg tttaatagca actgtaccac
    10741 gcataactgt aattaattgt tctttgtcat tcttaatttt tatatcccat acatgtgtcg
    10801 acttacctcg atgaataatt tcagctgtcg cagtaacacg accatcttta gcagaatgaa
    10861 tatggttagc attcatctct aaacctaatg gaataaattt ggttgtatca attaaattag
    10921 cagatcctaa tgaacatgct gtttcaccta aagcaatcga agccccacca tgtaaatatc
    10981 caaatggttg ttttacttta tcagtgacag gcatagaaat aacaactaaa ccatcttcct
    11041 ggtgatctat tgacatctca aatgtctcta ataaatgagt catattttca cctctttatg
    11101 cttatagtac catagcagca atagtaccga aaatcattaa aggaatatta taaaacaaga
    11161 agtttggtac acatgtatcg cgtatatgat catgttggcc atcaacattt aatcccgcag
    11221 ttggtcctaa tgttgaatca cttgcaggtg aacctgagtc acctaatgca ctcgctgttc
    11281 caatcaatgc gattaatgcc attgtatcta gtccaattga cgctccaaaa ggaatgaata
    11341 atgatgcgat aataggaatt gttgcaaatg atgatccaat acctaaagtg acaattaaac
    11401 ctatcacata catcatgata atgctaaata atttattatc accagtaata cttgttaaag
    11461 ttttaactaa ttcatctata tcaccagtag cattcattac accagcaaac ccatttgctg
    11521 ttaaaataac tacaccaata taagccataa ttttaatacc ttcaacaaac ttagcatcta
    11581 attcatacca attatatgca cgtgaaataa agaatacgag tacccctgcc agtgcaccaa
    11641 aaatcattga atctgtaaat gtttgtacta aaaatgtagc tagtattgcc acaattgtta
    11701 ctattaagat atatggtttt aactctgtaa cattgtcact atctgaaatt ttacgtgttt
    11761 catattcacg tggtttacga tatacatata aaccgataag taagccaaca atatacccca
    11821 ttgaaggaat aagcattgct ttccaaatca tattaaactc aattgggtga ttcgcctttg
    11881 caaagccact ttgaataatt tgctggaaaa tttgaccgaa tccatatggt aataacacat
    11941 acgggaaaca taaaccaaaa ccgataatca aaccgattaa acgtctatct atttttaagt
    12001 cattaaacag acttaacaat ggtgggatga caattggaat gaatgcaata tgtacaggaa
    12061 ttaagttttg actcatgata ctcatagcta ataatgcaat gattattgtc acttttactt
    12121 taactcttga ccatcgacta ttttcagcgt gaattgcatt tataattttt cctactaaat
    12181 agtctgtgat accactgtat gaaattaatg ctgcaaatcc acctaataat gcatagctta
    12241 atgctacctc agcaccatcg actatatttt tcccaaatac atttataact ttttcaatgc
    12301 tcatgcctga aattaagcca ccaactagcg cactgataaa taagcttata actacgttta
    12361 atcgacataa acatagcaca atcattaata ttactgctat tactactgca tttatcatat
    12421 gataaccatc ctttatcgct ctatcgaact aaagcgttta tagactacat agtatcagta
    12481 gctttaacac ctgtcaatag caaactatac ccataatctt tattttttag ttaattgtct
    12541 ttcttaaaat aattttcact ttcattaaat taaacaattt tacaagcctg gaacaccaat
    12601 caaaatccta agttctataa tgttatatta gtagtcgttg actgaatgag catatgatta
    12661 taataagttt ttgcaatgct aatcaagttg atttatgctc acaaggatat gcgatttata
    12721 ttttcttaca acaatgaaaa tgcctgatac aatgcgatcc tttatacttt caagagttaa
    12781 attagaaatg tataacaaga cacaagttgt atcaggcaaa taattaaatt attgttgttt
    12841 taaccattgt actatcgtcg gaatcataaa tcctgttggc ccttttggac cattatatga
    12901 agctttatta gtcgttgctg gacctgcaat atcgaaatga atgtgaggtg tttgaccact
    12961 aaaatgtgtt acaaaacttg ccgcaaatag cgctttacct tgtccattcg tatggttaac
    13021 taaatcagcg atatcactgt gattaatact tgcacgttcg gttgcagtaa tcggtaattc
    13081 aaataccatt tcatcgactt tagaacttat ttgtaatata tcgtttaata tcactttact
    13141 attcgattca aatgcagcag ctttatcatc gcctagtgca acgatggctg cacccgttaa
    13201 tgtagcaaag tccataatca cactaggctg atattgattt gcataataca cagcatctgc
    13261 aaggactaat ctaccttcag cgtctgtatt cattacttct acagtttcac cacttaatgc
    13321 tgtaaataca tcatctggct tcattgatgc ttcatttatc atattttcag cacaagcaag
    13381 cactccgaca atatttacag gcagttgtaa acgactagcc gcttcaatga taccaacgac
    13441 attcgcagcg ccacacatgt caaacttcat tgtagccatg ccattcttcg ttttaatact
    13501 ataaccacca gaatcatacg ttataccttt accaactaag gcaattggtg ctacatcttt
    13561 gtctttgcca ttatatgtga tggttactaa tctcggttta tgcttactac ctttacctac
    13621 tgcttgtaaa agtccgaatc cttcagaaac taaagtgtca taatctttaa catctacttt
    13681 gacctttgta tttttaaaat gattaacaat atcttctgca aatgtttgtg gtgttaatac
    13741 attcggtggc atattactaa agtctcttgc caaattaatg gattggccaa tactgatacc
    13801 ctcatgaatg aaatctaatt caattaatga ttcactaata aggtttaaat tcgtcttaaa
    13861 cggtgccttc ttacttgatt tataatgatc gaattcatat gttgcacgct cactttgaat
    13921 accgcatgcc attaatacat ctgataactg atcatatttt gaaataaatg aatccataag
    13981 taaataagta tcttcaatat gttctgactt tatgtattgg aaaagatgtc cccatatttt
    14041 caacatatct tgataactac gtgcttttaa gtttcctaaa ccaactgtaa ttaaacgata
    14101 tgtttgatct tgtacatcaa atgcagttgt ataaatcttc ccaactttac taccaataat
    14161 atgttgatgt tttagtcttt caagtgattc tgtaatatcg atatgattaa aactaatgcg
    14221 ctctaactga tttaaatgtt ctggtatacc aataatcaat gtatttattt cgttgcttag
    14281 tgtgttattt aatttaaaat tcataatgta cctccttaga tttgatatgt tactcacttc
    14341 aaattgtaca acaataaagc ccttcagtga cactgaagga ctttataaat gaataattta
    14401 attttatgtg tttagctgaa actttattta aagcctagaa tttacctttt ttgaatgcta
    14461 aaccgatacc accgattttg aataccgcac gtgtatcaat cacttttttc atgaatgctg
    14521 cttttttacc agcgataggt ttaccaaata ccatacctac accgtcatgt gaacctaaag
    14581 aacaaacagt tccacgatca acatattcga attcttcagt tgattcaccg tttaagatgc
    14641 gtttaatgtt tttagcaaca ctttcacctt gttgcattgc aatttgtgct gtagttggta
    14701 atggacgttc ttctccagct gggataaacg ctgaacagtc accaataaca aaaatgttgt
    14761 cgtaaccatt gattgttaaa tcttgctttg taacgatacg tccacgttta acgccttcaa
    14821 atgattcttc cattaattta ctaccacgta caccagctgc ccatactgaa gtacctgcat
    14881 ttaattgttg tttttcacca tctacttcaa ctacaaaacc tttttcgtta caagcaacga
    14941 ttggtgtagc aattttaaat tcaacaccgc ggtcttctaa gtagctaact gcgtggttaa
    15001 ctaattcttc tgagaacatt ggtaacattt taggtgctgc ttcaacacaa gtgattttaa
    15061 ctttattttg atccacacca tatttgctac ataattcagg aattctgtct gttaattcac
    15121 ctaagaattc aacaccagtg aatccagcac caccaactaa gatagataaa tcgttatcat
    15181 ctttttcttt tgatgctgca tagttagcaa atttgtcttc gatatgacgt gataattcac
    15241 gtgctgtgat aacattttca atttggaaag catgatcttt cataccttcg atgccgaatg
    15301 tttcactaac gaaacctaat gctactacta aaatatcaaa gtcataaata ccttgatttg
    15361 tttctacctt tttagcatca cggtcaattt ttgttacttc tgcttgaaca aagttcactt
    15421 tgtctttctt caagacactt tccacaggat ataatacatc ttcatagttt agtgtacctg
    15481 ctgatgcttc atgtaaccat gttgcttcat agtgatattc atttttatta ataagcgtaa
    15541 tttctgcttc ttctgttgat atcgcttttt gcaatttagt tacagtttgt aaacctgcat
    15601 aaccagcacc aagtacaagt acttttttac gatcttgagc catttaattt cacctaagct
    15661 ttcatatttt tttaaccaaa tgctgataat tactagtaac aactggtcat tactactttt
    15721 accacaatgt atactagttt atcatcattt tgttatttaa gtcttttaaa ttatgtaaaa
    15781 acctgtgatg ttcacaaaaa agacattttt aatccaatac taattctata gcttttatgt
    15841 tatattttca agcaagattc agtcagattg tgaaaaaatt aattaatttt ttcgtagcct
    15901 gatttcgatt aaaaatgtat ccgttatcac gatcatttaa attactattt atagtcagat
    15961 tcttaatttt caataagctt tttaataaaa caaaatatct ttacgtaaaa ataggacata
    16021 atcaaatatg tccctaatca taattgatat caatttgtat cccaaaaatt gggttaacca
    16081 ttaaaaagtt attttattag caattttcag gattacctgc aacttttgca gttctaaatg
    16141 aactaccaca gccacatgaa gcaattgcat taggattgtc gatttggaaa ccgccaccca
    16201 ttaatgattg cttaaaatca atagtcgtac catttaatac gggtgcatct tctttgtcta
    16261 ctaatacttt taatccaaag tattctaaga cttcatcatt ttcaccaggc gcttcttctg
    16321 cacccatacc gtatgttaaa ccagtgcacc cgccaccatt cactttaatt tttaaatagc
    16381 catctggcat ttcatttgct ttaagcatat cttttacttc gtaggcagct gcttctgtta
    16441 atataactgt tggcatgata actcctcctt aaaaaatcca tgtttctttt atatgtgcat
    16501 atatattttg taataattct tccggcgaat caccttcaac aatatcacca tttactaaag
    16561 catacaaccc ggctgaacat ataccacaat gtgtcaggca accatactct aacacatcga
    16621 catctgggtc attttccagt tgattaaaaa cataatctcc accttttgcc atgttagaga
    16681 gacaaaattc tacgatcgga ttcatacttc accttcttat ttcatttgtt acaatattat
    16741 agcattttaa aactggtatt ttaacatgat gtgctcaatt accaacaact gatgtttctt
    16801 atcccagtta tgtaatagtg ccttagttag tacaaatatt tatttgtagt cattttcaaa
//
LOCUS       DANBWH010000038        16473 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.040, whole genome shotgun sequence.
ACCESSION   DANBWH010000038 DANBWH010000000
VERSION     DANBWH010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16473
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.040"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            41..889
                     /locus_tag="PPY92_000875"
     CDS             41..889
                     /locus_tag="PPY92_000875"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDJ2624894.1"
                     /translation="MIYETAPAKINFTLDTLFKRNDGYHEIEMIMTTVDLNDRLTFHK
                     RKDRKIVVEIEHNYVPSNHKNLAYRAAQLFIEQYQLKQGVTISIDKEIPVSAGLAGGS
                     ADAAATLRGLNRLFDIGASLEELALLGSKIGTDIPFCIYNKTALCTGRGEKIEFLNKP
                     PSAWVILAKPNLGISSPDIFKLINLDKRYDVHTKMCYEALENRDYQQLCQSLSNRLEP
                     ISVSKHPQIDKLKNNMLKSGADGALMSGSGPTVYGLARKESQAKNIYNAVNGCCNEVY
                     LVRLLG"
     gene            903..1727
                     /gene="purR"
                     /locus_tag="PPY92_000876"
     CDS             903..1727
                     /gene="purR"
                     /locus_tag="PPY92_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDJ2624895.1"
                     /translation="MRYKRSERIVFMTQYLMNHPNKLIPLTFFVKKFKQAKSSISEDV
                     QIIKNTFQKEKLGTVITTAGASGGVTYKPMMSKEEATEVVNEVITLLEEKERLLPGGY
                     LFLSDLVGNPSLLNKVGKLIASIYMEEKLDAVVTIATKGISLANAVANILNLPVVVIR
                     KDNKVTEGSTVSINYVSGSSRKIETMVLSKRTLAENSNVLVVDDFMRAGGSINGVMNL
                     MNEFKAHVKGVSVLVESKEVKQRLIEDYTSLVKLSDVDEYNQEFNVEPGNSLSKFS"
     gene            1744..2124
                     /locus_tag="PPY92_000877"
     CDS             1744..2124
                     /locus_tag="PPY92_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDJ2624896.1"
                     /translation="MKIINTTRLPEALGPYSHATVVNGMVYTSGQIPLNVDGKIVSAD
                     VQAQTKQVLENLKVVLEEAGSDLNSVAKATIFIKDMNDFQKINEVYGQYFNEHKPARS
                     CVEVARLPKDVKVEIELVSKIKEL"
     gene            2196..2498
                     /gene="spoVG"
                     /locus_tag="PPY92_000878"
     CDS             2196..2498
                     /gene="spoVG"
                     /locus_tag="PPY92_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HDJ2624897.1"
                     /translation="MKVTDVRLRKIQTDGRMKALVSITLDEAFVIHDLRVIEGNSGLF
                     VAMPSKRTPDGEFRDIAHPINSDMRQEIQDAVMKVYDETDEVVPDKNATSEDSEEA"
     gene            2709..2840
                     /locus_tag="PPY92_000879"
     CDS             2709..2840
                     /locus_tag="PPY92_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624898.1"
                     /translation="MRLNDARIFEVRKKLFLKLQRIKNNAFYMLKEYCRLNYNNDEV"
     gene            2863..4215
                     /gene="glmU"
                     /locus_tag="PPY92_000880"
     CDS             2863..4215
                     /gene="glmU"
                     /locus_tag="PPY92_000880"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDJ2624899.1"
                     /translation="MRRHAIILAAGKGTRMKSKKYKVLHEVAGKPMVEHVLESVKGSG
                     VDQVVTIVGHGAESVKGHLGERSLYSFQEKQLGTAHAVQMAKSHLEDKEGTTIVVCGD
                     TPLITKETLETLIAHHEDANAQATVLSASIQQPYGYGRIVRNASGRLERIVEEKDATQ
                     AEKDINEISSGIFAFNNKTLFEKLTQVKNDNAQGEYYLPDVLSLILNDGGIVEVYRTN
                     DVEEIMGVNDRVMLSQAEKAMQRRTNHYHMLNGVTIIDPDSTFIGPDVTIGSDTVIEP
                     GVRINGRTEIGEDVVIGQYSEINNSTIENGACIQQSVVNDASVGANTKVGPFAQLRPG
                     AQLGADVKVGNFVEIKKADLKDGAKVSHLSYIGDAVIGERTNIGCGTITVNYDGENKF
                     KTIVGKDSFVGCNVNLVAPVTIGDDVLVAAGSTITDDVPNDSLAVARARQTTKEGYRK
                     "
     gene            4362..5327
                     /locus_tag="PPY92_000881"
     CDS             4362..5327
                     /locus_tag="PPY92_000881"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDJ2624900.1"
                     /translation="MLNNEYKNSSLKIFSLKGNEALAQEVADQVGIELGKCSVKRFSD
                     GEIQINIEESIRGCDVFIIQPTSYPVNLHLMELLIMIDACKRASAATINIVVPYYGYA
                     RQDRKARSREPITAKLVANLIETAGATRMIALDLHAPQIQGFFDIPIDHLMGVPILAK
                     HFKDDPNINPEECVVVSPDHGGVTRARKLADILKTPIAIIDKRRPRPNVAEVMNIVGE
                     IEGRTAIIIDDIIDTAGTITLAAQALKDKGAKEVYACCTHPVLSGPAKERIENSAIKE
                     LIVTNSIHLDEDRKPSNTKELSVAGLIAQAIIRVYERESVSVLFD"
     gene            5477..6130
                     /locus_tag="PPY92_000882"
     CDS             5477..6130
                     /locus_tag="PPY92_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HDJ2624901.1"
                     /translation="MASLKSIIRQGKQTRSDLKQLRKSGKVPAVVYGYGTKNVSVKVD
                     EVEFIKVIREVGRNGVIELGVGSKTIKVMVADYQFDPLKNQITHIDFLAINMSEERTV
                     EVPVQLVGEAVGAKEGGVVEQPLFNLEVTATPDNIPEAIEVDITELNINDSLTVADVK
                     VTGDFKIENDSAESVVTVVAPTEEPTEEEIEAMEGEQQTEEPEVVGESKEDEEKTEE"
     gene            6441..7013
                     /locus_tag="PPY92_000883"
     CDS             6441..7013
                     /locus_tag="PPY92_000883"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDJ2624902.1"
                     /translation="MKCIVGLGNIGKRFELTRHNIGFEVVDYILEKNNFSLDKQKFKG
                     AYTIERMNGDKVLFIEPMTMMNLSGEAVAPIMDYYNVNPEDLIVLYDDLDLEQGQVRL
                     RQKGSAGGHNGMKSIIKMLGTDQFKRIRIGVGRPTNGMTVPDYVLQRFSNDEMVTMEK
                     VIEHAARAIEKFVETSRFDHVMNEFNGEVK"
     gene            7013..10513
                     /gene="mfd"
                     /locus_tag="PPY92_000884"
     CDS             7013..10513
                     /gene="mfd"
                     /locus_tag="PPY92_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDJ2624903.1"
                     /translation="MTILTTLIKEDNHFQDLNQVFGQANTLVTGLSPSAKVTMIAEKY
                     AQSNQQLLLITNNLYQADKLETDLLQFIDAEELYKYPVQDIMTEEFSTQSPQLMSERI
                     RTLTALAQGKKGLFIVPLNGLKKWLTPVEMWQNHQMTLRVGEDIDVDQFLNKLVNMGY
                     KRESVVSHIGEFSLRGGIIDIFPLIGEPIRIELFDTEIDSIRDFDVETQRSKDIIEEV
                     DITTASDYIITEEVIRHLKEELKTAYENTRPKIDKSVRNDLKETYESFKLFESTYFDH
                     QILRRLVAFMYETPSTIIEYFQKDAIIAVDEFNRIKETEESLTVESDSFISNIIESGN
                     GFIGQSFIKYDDFETLIEGYPVTYFSLFATTMPIKLNHIIKFSCKPVQQFYGQYDIMR
                     SEFQRYVNQNYHIVVLVETETKRMQAMLSEMHIPSITKLHRSMSSGQAVIIEGSLSEG
                     FELPDMGLVVITERELFKSKQKKQRKRTKAISNAEKIKSYQDLNVGDYIVHVHHGVGR
                     YLGVETLEVGQTHRDYIKLQYKGTDQLFVPVDQMDQVQKYVASEDKTPKLNKLGGSEW
                     KKTKAKVQQSVEDIAEELIDLYKEREMAEGYQYGEDTAEQTTFELDFPYELTPDQAKS
                     IDEIKDDMQKSRPMDRLLCGDVGYGKTEVAVRAAFKAVMEGKQVAFLVPTTILAQQHY
                     ETLIERMQDFPVEIQLMSRFRTPKEIKQTKEGLKTGFVDIVVGTHKLLSKDIQYKDLG
                     LLIVDEEQRFGVRHKERIKTLKHNVDVLTLTATPIPRTLHMSMLGVRDLSVIETPPEN
                     RFPVQTYVLEQNMSFIKEALERELSRDGQVFYLYNKVQSIYEKREQLQMLMPDANIAV
                     AHGQMTERDLEETMLSFINNEYDILVTTTIIETGVDVPNANTLIIEDADRFGLSQLYQ
                     LRGRVGRSSRIGYAYFLHPANKVLTETAEDRLQAIKEFTELGSGFKIAMRDLNIRGAG
                     NLLGKQQHGFIDTVGFDLYSQMLEEAVNEKRGIKEPESEVPEVEVDLNLDAYLPTEYI
                     ANEQAKIEIYKKLRKTETFDQIIDIKDELIDRFNDYPVEVARLLDIVEIKVHALHSGI
                     TLIKDKGKIIDIHLSVKATENIDGEVLFKATQPLGRTMKVGVQNNAMTITLTKQNQWL
                     DSLKFLVKCIEESMRISDEA"
     gene            10503..12029
                     /locus_tag="PPY92_000885"
     CDS             10503..12029
                     /locus_tag="PPY92_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDJ2624904.1"
                     /translation="MKHKEAFNGVVVLTAALIVIKILSAVYRIPYQNILGDTGLYAYQ
                     QVYPIVALGMILSMNTIPSAITQNIGEYHSDEAYAKAVAYIQLVGILLFIAIFVFANN
                     IAHMMGDSHLTPMIQAASLSFIFIGMLGVLRGYYQSANNMTVPAISQVIEQVIRVGII
                     IVTIVIFVDRGWTIYEAGTIAILASTIGFLGSSIYLVAHRPFKFKMVNNTAKIVWKQF
                     ALSVLIFAISQLIVILWQVIDSVTIIKSLQAIRVPFDVAITEKGVYDRGASFIQMGLI
                     VTTTFSFALIPLLSDAIKMNNQVLMNRYANASLKITILISTAAGIGLINLLPLMNGVF
                     FKTNDLTLTLSVYMITVICVSLIMMDMALLQAQHAVRPIFVGMTAGLVIKFILNIILI
                     RLSGIIGASISTVVSLIIFGTIIHIAVTRKYHLHAMRRFFINVVLGMVFMSIVVQCVL
                     NIVTTHGRFTGLIELLCAAVLGIIALFFYIFRFNVLTYKELTYLPFGSKLYQIKKGRR
                     "
     gene            12029..13222
                     /locus_tag="PPY92_000886"
     CDS             12029..13222
                     /locus_tag="PPY92_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDJ2624905.1"
                     /translation="MAHTITIVGLGNYGIDDLPLGIYKFLKTQDKVYARTLDHPVIES
                     LQDELTFQSFDHVYEAHDQFEDVYNDIVAQLVEAANEKDIVYAVPGHPRVAETTTVKL
                     LALAKDNTDIDVKVLGGKSFIDDVFEAVNVDPNDGFTLLDATSLQEVILNVRTHTLIT
                     QVYSAMVAANLKITLMERYPDDYPVQIVTGARSDGADNVVTCPLYELDHDENAFNNLT
                     SVFVPKIITSTYLYHDFDFATEVIDTLVDEDKGCPWDKVQTHETLKRYLLEETFELFE
                     AIDNEDDWHMIEELGDILLQVLLHTSIGKKEGYIDIKEVITSLNAKMIRRHPHIFGDA
                     NAETIDDLKEIWSKAKDAEGKQPRVKFEKVFAEHFLNLYEKTKDKSFDEASLKQWLEK
                     GESNT"
     gene            13219..13482
                     /locus_tag="PPY92_000887"
     CDS             13219..13482
                     /locus_tag="PPY92_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDJ2624906.1"
                     /translation="MRLDKYLKVSRLIKRRTLAKEVSDQGRITINGNVAKAGSDVKVE
                     DVLTIRFGQKLVTVKVTALNEHASKDNAKGMYEIIEERRLEEA"
     gene            13500..13892
                     /locus_tag="PPY92_000888"
     CDS             13500..13892
                     /locus_tag="PPY92_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDJ2624907.1"
                     /translation="MKNKVEHIENQYTSQENKKKQRQKMKMRVVRRRITVFAGVLLAI
                     IVVLSILLVVQKHRNDIDAQERKAKEAQFQKQQNEEIALKEKLNNLNDKDYIEKIARD
                     DYYLSNKGEVIFRLPEDKDSSSSKSSKK"
     gene            13997..14398
                     /locus_tag="PPY92_000889"
     CDS             13997..14398
                     /locus_tag="PPY92_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HDJ2624908.1"
                     /translation="MSIEVGNKLKGKVTGIKKFGAFVELPEGKSGLVHISEVADNYVE
                     NVEEHLSVGDEVDVKVLSIADDGKISLSIKKAKDRPRRQHTSKPSHQKPVQKAEDFEK
                     KLSNFLKDSEDKLTSIKRQTESRRGGKGSRR"
     gene            14578..15873
                     /gene="tilS"
                     /locus_tag="PPY92_000890"
     CDS             14578..15873
                     /gene="tilS"
                     /locus_tag="PPY92_000890"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDJ2624909.1"
                     /translation="MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTC
                     LHVNHGVRSASIEEARFLEAYCERHHIDLHIKKLDLSHSLDRNNSIQNEARIKRYEWF
                     DEMMNVLEADVLLTAHHLDDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLA
                     VSKKEIKQFQERYHIPYFEDESNKDNKYIRNDIRNRIIPAIDENNQLKVSHLLKLKQW
                     HDEQYDILQYSAKQFIQEFVKFDEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYEL
                     FNISAKTYEEWFKQFSSKKAQFSINLTDKWIIQIAYGKLIIMAKNNGDTYFRVQTIKK
                     PGNYFFNKYRLEIHSNLPKCLFPLTVRTRQSGDTFKLNGRDGYKKVNRLFIDCKVPQW
                     VRDQMPIVLDKQQRIIAVGDLYQQQTIKKWIIISKNGDE"
     gene            15878..16417
                     /gene="hpt"
                     /locus_tag="PPY92_000891"
     CDS             15878..16417
                     /gene="hpt"
                     /locus_tag="PPY92_000891"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDJ2624910.1"
                     /translation="MHNDLKEVLLTEEDIQNICKELGAQLTKDYQGKPLVCVGILKGS
                     AMFMSDLIKRIDTHLSIDFMDVSSYHGGTESTGEVQIIKDLGSSIENKDVLIIEDILE
                     TGTTLKSITELLQSRKVNSLEIVTLLDKPNRRKADIEAKYVGKKIPDEFVVGYGLDYR
                     ELYRNLPYIGTLKPEVYSN"
BASE COUNT         5913 a         2225 c         3315 g         5020 t
ORIGIN      
        1 ttaaaatcat catattaaga taacgaaaag agggcggaaa atgatatatg aaacggcacc
       61 agccaaaatt aattttacgc tcgatacact ttttaaaaga aatgatggct atcatgagat
      121 tgaaatgata atgacaacag ttgatttaaa tgatcgttta acttttcata aaagaaaaga
      181 tcgaaagata gttgttgaga ttgaacataa ttatgtgcct tctaatcata aaaatctcgc
      241 atatcgtgca gcgcaactat ttattgagca atatcaacta aagcaaggtg taacaatttc
      301 tatcgataaa gaaatacctg tttctgctgg cttagctgga ggttcggctg atgcagcagc
      361 aacgttaaga ggattgaatc gactttttga tataggggcg agtttggaag aattggctct
      421 actaggcagt aaaattggga cagatattcc gttttgtatt tataataaaa ctgcactatg
      481 tactggaaga ggagagaaaa tcgagttttt aaataaacca ccttcagctt gggtgattct
      541 tgctaaacca aacttaggca tatcatcacc agatatattt aagttgatca atttagataa
      601 gcgttacgac gtacatacga aaatgtgtta tgaggcctta gaaaatcgag attatcaaca
      661 attatgtcaa agtttgtcta atcgattaga gccaatttct gtttcaaaac acccacaaat
      721 cgataaatta aaaaataata tgttgaaaag tggtgcagat ggtgcgttaa tgagtggaag
      781 cggaccgact gtgtatgggc tagcacgaaa agaaagccaa gcaaaaaata tttataatgc
      841 agttaacggt tgttgtaatg aagtgtactt agttagacta ttaggataga agggttgaaa
      901 agatgagata taaacgaagc gagagaattg tttttatgac gcaatatttg atgaaccatc
      961 cgaataaatt gattccatta actttttttg tgaaaaaatt taaacaggcg aaatcttcaa
     1021 taagtgaaga tgtccaaatt ataaaaaata cattccaaaa agaaaagtta ggtacagtaa
     1081 ttactactgc tggcgcaagt ggtggtgtta cgtataaacc aatgatgagt aaagaagagg
     1141 cgactgaagt tgttaatgag gtcattactc tattagaaga gaaagaacgt ttgttacctg
     1201 gcgggtattt atttttatca gatttggtag gtaatccatc gctactaaac aaagttggta
     1261 agttaattgc cagtatttac atggaagaaa aattagatgc tgttgttacc attgcgacaa
     1321 aaggtatttc attggcaaat gcggttgcta atattttaaa tttaccagta gtagtgatta
     1381 gaaaagacaa caaggtgact gaaggttcta cagtttcaat taattacgtt tcaggatctt
     1441 caagaaaaat agagacaatg gtactttcga agagaacttt agcagaaaat tcaaatgttt
     1501 tagttgtcga tgattttatg agggctggtg gctctattaa tggtgttatg aatttaatga
     1561 atgagtttaa agcccatgta aaaggggtat cagtacttgt agaatcaaaa gaagttaaac
     1621 aaagattgat tgaagattat acttccttag tgaaattatc tgatgtagat gaatataatc
     1681 aagagtttaa cgtagaacct ggcaacagtt tatctaagtt ttcataaaag gagttttagt
     1741 attatgaaaa tcattaacac aacaagatta ccggaagcac ttggaccata ttcgcatgca
     1801 acagttgtga atggtatggt ttatacttct ggtcagattc cattgaatgt tgatgggaaa
     1861 atcgtaagcg ctgatgttca agcacagaca aaacaagttt tagaaaattt aaaggttgtt
     1921 ttggaagaag caggatctga tttgaattct gttgcgaaag cgaccatttt cattaaagat
     1981 atgaatgatt tccaaaaaat aaatgaagtg tatggtcaat attttaatga acacaagcca
     2041 gcgcgtagtt gtgtagaggt tgcgcgtttg ccaaaagatg tgaaagtaga aattgaatta
     2101 gtaagtaaaa ttaaggaatt ataattttcg attaatatgt ttaatcaagc ttctaaataa
     2161 aacagagaga tatatactat aggggggctc actacatgaa agtgacagat gtaagactta
     2221 gaaaaataca aacagatgga cgaatgaaag cactcgtttc cattacatta gatgaagctt
     2281 tcgtaattca tgatttacgt gtaattgaag gaaactctgg cttgttcgtt gcaatgccaa
     2341 gtaaacgtac accagatggt gaattccgcg acatcgcgca tcctattaat tcagatatga
     2401 gacaagaaat tcaagatgca gtgatgaaag tatatgatga aacagatgaa gtagtaccag
     2461 ataaaaacgc tacatcagaa gattcagaag aagcttaatc aattttatat ttagcgatgt
     2521 aatacatttg caataagttg atttgatact gtcgataaag cataaagctt tgtcggcagt
     2581 ttttttagtt tgtattaatg tttttttagt ttgtattaat gtttttttat ttttaatgaa
     2641 aggctaataa atatatacgt taacagatta tgatgatatg aaaattattg attcattgct
     2701 gataaaaatt gcgtttgaat gatgctcgta tttttgaagt aagaaaaaag ttgtttttaa
     2761 aattacaacg aattaaaaac aatgcctttt atatgttgaa agagtattgc agattaaatt
     2821 ataataatga cgaagtgtaa aatttaatgg gggttaatgt tcatgcgaag acacgcgata
     2881 attttggcag caggtaaagg cacaagaatg aaatctaaaa agtataaagt gctacacgag
     2941 gttgctggga aacctatggt cgaacatgta ttggaaagtg tgaaaggctc tggtgtcgat
     3001 caagttgtaa ccatcgtagg acatggtgct gaaagtgtaa aaggacattt aggcgagcgt
     3061 tctttataca gttttcaaga gaaacaactc ggtactgcgc atgcagtgca aatggcgaaa
     3121 tcacacttag aagacaagga aggtacgaca atcgttgtat gtggtgacac accgctcatc
     3181 acaaaggaaa cattagaaac attgattgcg catcatgagg atgctaatgc tcaagcaact
     3241 gtattatctg catcgattca acaaccatat ggatacggaa gaatcgttcg aaatgcgtca
     3301 ggtcgtttag aacgcatagt tgaagagaaa gatgcaacgc aagctgaaaa ggatattaat
     3361 gaaattagtt caggtatttt tgcgtttaat aataaaacgt tgtttgaaaa attaacacaa
     3421 gtgaaaaatg ataatgcgca aggtgaatat tacctccctg atgtattgtc gttaatttta
     3481 aatgatggcg gcatcgtaga agtgtatcgt accaatgatg ttgaagaaat catgggtgta
     3541 aatgatcgtg taatgcttag tcaggctgag aaggcgatgc aacgtcgtac gaatcattat
     3601 cacatgctaa atggtgtgac aatcatcgat cctgacagca cttttattgg tccagacgtt
     3661 acaattggta gtgatacagt cattgaacca ggcgtacgaa ttaatggtcg tacagaaatt
     3721 ggcgaagatg ttgttattgg tcagtactct gaaattaaca atagtacgat tgaaaatggt
     3781 gcatgtattc aacagtctgt tgttaatgat gctagcgtag gagctaatac taaggtcgga
     3841 ccgtttgcgc aattgagacc aggcgcgcaa ttaggtgcag atgttaaggt tggaaatttt
     3901 gtagaaatta aaaaagcaga tcttaaagat ggtgccaagg tttcacattt aagttatatt
     3961 ggcgatgctg taattggcga acgtactaat attggttgcg gaacgattac agttaactat
     4021 gatggtgaaa ataaatttaa aactatcgtc ggcaaagatt catttgtagg ttgcaatgtt
     4081 aatttagtag cacctgtaac aattggtgat gatgtattgg tggcagctgg ttccacaatc
     4141 acagatgacg taccaaatga cagtttagct gtggcaagag caagacaaac aacaaaagaa
     4201 ggatatagga aataatcatt tacgtattta aaatggctag gataaaagga taatcctatg
     4261 taatattaat gtaatcttta tgatttaatg attcgcatag taatggagtt acattttata
     4321 tataatagta attgcgtaag taaataattg gaggactata aatgttaaat aatgaatata
     4381 agaattcgtc attaaagatt ttttcattga aaggaaacga agcattagcg caagaagttg
     4441 ctgaccaagt aggaattgaa ctaggtaaat gttcagttaa acgttttagt gatggagaaa
     4501 ttcaaattaa tatcgaagag agtattcgtg gttgtgacgt atttattatt caaccaacat
     4561 catatcctgt gaatctacat ttaatggaat tattaattat gattgatgct tgtaaacgtg
     4621 cttctgcagc aacaatcaat attgtagtgc catattatgg atatgcaaga caagatagaa
     4681 aagcccgtag ccgtgagcca atcactgcta aattagttgc aaacttaatc gaaacagctg
     4741 gcgcaactcg tatgattgcg ttagacttac atgcaccaca aattcaagga ttctttgata
     4801 ttccaattga ccacttaatg ggtgtgccaa ttcttgctaa acatttcaaa gatgatccga
     4861 atattaaccc agaagaatgt gtcgttgttt caccagacca tggcggtgtt acacgtgcac
     4921 gtaaattagc tgacatttta aaaactccaa ttgcaattat agataaacgt cgtcctagac
     4981 caaatgttgc tgaagtgatg aacattgttg gtgagattga aggacgtacg gcaattatta
     5041 ttgacgatat tattgataca gcaggtacaa tcactttagc tgcacaagca ttaaaagata
     5101 aaggtgctaa agaagtatat gcttgttgta cacaccctgt tttatcagga ccggctaaag
     5161 aacgtatcga aaattctgct ataaaagaat taatcgtaac aaactcaatt catttagatg
     5221 aagatcgcaa accatctaac actaaagaat tatctgttgc tggtttaatc gcacaagcta
     5281 tcattcgtgt atacgaaaga gaatcagtta gcgtattatt tgactaatat ttaaaaggcg
     5341 tttgacgaac atattccaaa cgtgtataat agtttcgttc gtgattatac gaataaataa
     5401 acacttgcaa gcaacgatga tgttgatggg taagtgaggt gctcgttttg agcaaaaatg
     5461 aaaggtggaa atgagaatgg cttcattaaa gtcaatcatc cgtcaaggta aacaaacacg
     5521 ttcagatctt aaacaattaa gaaaatctgg taaagtacca gcagtagtat acggttacgg
     5581 tactaaaaac gtgtcagtta aagttgatga agtagaattc atcaaagtta tccgtgaagt
     5641 aggtcgtaac ggtgttatcg aattaggcgt tggttctaaa actatcaaag ttatggttgc
     5701 agactaccaa ttcgatccac ttaaaaacca aattactcac attgacttct tagcaatcaa
     5761 tatgagtgaa gaacgtactg ttgaagtacc agttcaatta gttggtgaag cagtaggcgc
     5821 taaagaaggc ggcgtagttg aacaaccatt attcaactta gaagtaactg ctactccaga
     5881 caatattcca gaagcaatcg aagtagacat tactgaatta aacattaacg acagtttaac
     5941 tgttgctgat gttaaagtaa ctggcgactt caaaatcgaa aacgattcag ctgaatcagt
     6001 tgtaacagta gttgctccaa ctgaagaacc aactgaagaa gaaatcgaag ctatggaagg
     6061 cgaacaacaa actgaagaac cagaagttgt tggcgaaagc aaagaagacg aagaaaaaac
     6121 tgaagagtaa ttttaatctg ttacattaaa gtttttatac tttgtttaac aagcactgtg
     6181 cttattttaa tataagcatg gtgctttttg tgttattata aagcttaatt aaactttatt
     6241 actttgtact aaagtttaat taattttagt gagtaaaaga tattaaactc aacaatgata
     6301 catcataaaa attttaatgt actcgatttt aaaatacata cttactaagc taaagaataa
     6361 tgataattga tggcaatggc ggaaaataga tgttgtcatt ataataataa atgaaacaat
     6421 tatgttggag gtaaacacgc atgaaatgta ttgtaggtct aggtaatata ggtaaacgtt
     6481 ttgaacttac aagacataat atcggctttg aagtcgttga ttatatttta gagaaaaata
     6541 atttttcatt agataaacaa aagtttaaag gtgcatatac aattgaacga atgaacggag
     6601 ataaagtgtt atttatcgaa ccaatgacaa tgatgaattt gtcaggagaa gcagttgcac
     6661 cgattatgga ttattacaat gttaatccag aagatttaat tgtcttatat gatgatttag
     6721 atttagaaca aggacaagtt cgcttaagac aaaaaggaag tgcgggcggt cacaatggta
     6781 tgaaatcaat tattaaaatg cttggtacag accaatttaa acgtattcgt attggtgtgg
     6841 gaagaccaac gaatggtatg acggtacctg attatgtttt acaacgcttt tcaaatgatg
     6901 aaatggtaac gatggaaaaa gttatcgaac acgcagcacg cgcaattgaa aagtttgttg
     6961 aaacatcacg atttgaccat gttatgaatg aatttaatgg tgaagtgaaa taatgacaat
     7021 attgacaacg cttataaaag aagataatca ttttcaagac cttaatcagg tatttggaca
     7081 agcaaacaca ctagtaactg gtctttcccc gtcagctaaa gtgacgatga ttgctgaaaa
     7141 atatgcacaa agtaatcaac agttattatt aattaccaat aatttatacc aagcagataa
     7201 attagaaaca gatttacttc aatttataga tgctgaagaa ttgtataagt atcctgtgca
     7261 agatattatg accgaagagt tttcaacaca aagccctcaa ctgatgagtg aacgtattag
     7321 aactttaact gcgttagctc aaggtaagaa agggttattt atcgttcctt taaatggttt
     7381 gaaaaagtgg ttaactcctg ttgaaatgtg gcaaaatcac caaatgacat tgcgtgttgg
     7441 tgaggatatc gatgtggacc aatttcttaa caaattagtt aatatggggt acaaacggga
     7501 atccgtggta tcgcatattg gtgaattctc attgcgagga ggtattatcg atatctttcc
     7561 gctaattggg gaaccaatca gaattgagct atttgatacc gaaattgatt ctattcgcga
     7621 ttttgatgtt gaaacgcagc gttccaaaga tattattgaa gaagtcgata tcacaactgc
     7681 aagtgattat atcattactg aagaagtgat tagacatctt aaagaagagt tgaaaactgc
     7741 atatgaaaat acaagaccca aaatagataa atcagtgcgc aatgatctga aagaaacgta
     7801 tgaaagcttt aaattattcg aaagtacata ctttgatcat caaatactac gtcgtttagt
     7861 agcgtttatg tatgaaacac cttcgacaat tattgagtat ttccaaaaag atgcaatcat
     7921 tgcagttgat gaatttaatc gtattaaaga aactgaagaa agtttaacag tagagtctga
     7981 ttcgtttatt agcaatatta ttgaaagtgg taatggattt ataggacaaa gttttataaa
     8041 atatgatgat tttgaaacat tgattgaagg ctatcctgtc acttattttt cattattcgc
     8101 tacaacaatg ccgataaaac taaatcatat tattaaattt tcatgtaaac ctgtccaaca
     8161 attttatggg caatatgaca ttatgcgttc tgaatttcaa cgatatgtca atcaaaatta
     8221 tcatatcgtg gttttggtcg aaactgaaac taaacgtatg caagcgatgt taagtgaaat
     8281 gcatattcca tcaataacaa aattgcatcg ctcaatgtca tcggggcaag cagtgattat
     8341 tgaaggcagt ttatctgaag gatttgaact acctgatatg ggattagttg tcattactga
     8401 gcgtgagctt tttaaatcaa aacagaaaaa gcaacgaaaa cgtacgaaag ctatctcaaa
     8461 tgctgaaaaa attaagtctt accaagattt aaatgtggga gattatattg ttcatgtgca
     8521 tcatggtgtt ggtagatatt taggtgttga gacgctcgaa gtggggcaaa cgcatcgtga
     8581 ttatattaaa ttgcaatata aaggtacgga tcaactattt gttccagtag atcaaatgga
     8641 tcaagttcaa aaatatgtag cttcggaaga taagacgcca aaattaaata aactcggtgg
     8701 cagtgaatgg aaaaaaacaa aagcaaaagt tcaacaaagt gttgaagata ttgctgaaga
     8761 gttgattgat ttatataaag aaagagaaat ggcagaaggt tatcaatatg gggaagacac
     8821 agctgagcaa acaacatttg aattagattt tccatatgaa cttacgcctg accaagctaa
     8881 atctatcgat gaaattaaag atgacatgca aaaatcgcgt ccaatggatc gcttgctatg
     8941 tggtgatgtt ggttatggta aaactgaagt tgcagtgaga gcagcattca aagctgtaat
     9001 ggaaggaaag caagttgcat ttttagttcc tacaactatt ttagctcagc aacattatga
     9061 gacgttaatt gagcgtatgc aagattttcc tgttgaaatt caattaatga gtcgttttag
     9121 aacgccgaaa gagataaaac aaactaagga aggacttaaa actgggtttg ttgacatagt
     9181 tgttggtaca cacaaattac ttagtaaaga tatacagtat aaagatttag ggctgttgat
     9241 tgtagatgaa gaacaacgat ttggtgtacg ccataaagag cgtattaaaa cattaaaaca
     9301 taatgtagat gtactaacat tgactgcaac cccaatacct agaacattgc atatgagtat
     9361 gctaggtgtg cgcgatttgt cagtgattga aacgccgcca gaaaatcgtt tcccagttca
     9421 aacatatgta ttagaacaga acatgagttt tatcaaagaa gctttagaaa gagaactatc
     9481 ccgtgatggc caagtgtttt atctttataa taaagtgcaa tccatttatg aaaaacgaga
     9541 acaactccag atgttaatgc cagatgctaa cattgcagtt gctcatggac aaatgacaga
     9601 gcgcgattta gaagaaacga tgttaagttt tatcaataat gaatatgata ttttagtaac
     9661 gacgacgatt attgaaacag gtgtcgatgt cccaaatgca aatactttga tcattgaaga
     9721 tgcagatcgc tttggattga gtcagttgta tcaattaaga ggtcgtgttg gtcgttcaag
     9781 tcgtattggt tatgcatact tcttacatcc agcaaataag gtactaactg agactgcaga
     9841 agatcgatta caagcgatta aagaatttac ggagttaggc tcaggattta agattgcgat
     9901 gcgtgatttg aacattcgtg gtgctggtaa tttgttaggt aaacaacagc acggctttat
     9961 tgatacagtt ggatttgatt tgtacagtca aatgttagaa gaagctgtaa atgaaaaacg
    10021 tggtattaag gaaccagaat ctgaggtgcc agaagtcgaa gttgatttaa acttggatgc
    10081 atatttgcca acagaatata ttgcaaatga acaagctaaa attgaaattt ataaaaagct
    10141 acgaaaaact gaaacatttg atcaaattat cgacattaaa gatgaattaa ttgatcgttt
    10201 taatgattat cctgttgaag tagcacgttt gcttgatata gtggaaataa aagtacacgc
    10261 attacattca ggtatcacgt tgattaaaga taaagggaaa ataattgata ttcatttatc
    10321 tgtaaaagcc actgaaaata ttgatggcga agtgctgttc aaagcaacac aacctttagg
    10381 tagaacaatg aaggttggtg ttcaaaataa tgcaatgaca attactttaa cgaaacaaaa
    10441 tcaatggctt gatagtttga agtttttagt taagtgcatt gaagaaagca tgagaatcag
    10501 tgatgaagca taaagaagca tttaatggcg ttgtcgtgtt aactgctgca ttaattgtca
    10561 ttaaaattct gagtgctgta tatcgaattc catatcaaaa tatattaggt gatacaggtt
    10621 tgtatgcata tcaacaagtg tatccaattg tagcattagg aatgatatta tcgatgaata
    10681 ccattcctag tgcaattaca caaaatatag gggagtatca tagtgacgaa gcatatgcaa
    10741 aagcggtcgc ttatatacaa ttagttggta tattattatt tattgctatt tttgtgtttg
    10801 cgaacaatat tgcacatatg atgggtgata gccatttaac accaatgatt caagcagcaa
    10861 gtttaagctt tatatttata ggtatgcttg gcgtgttaag aggttattat caatctgcaa
    10921 ataatatgac agttccggct atttcccagg ttatagaaca agttatacga gtaggtatta
    10981 tcattgttac tattgttatt tttgtagaca gaggttggac gatatatgaa gcgggaacaa
    11041 ttgctatttt agcatcaacg ataggttttt taggttcttc aatttattta gtagcgcacc
    11101 gaccttttaa gtttaaaatg gtaaataaca ctgcaaagat tgtttggaaa cagttcgcac
    11161 tttcggtttt gattttcgct atcagtcaat taatcgtaat tttatggcaa gtgattgata
    11221 gtgttactat tattaagtca cttcaagcga tacgcgtgcc attcgatgtt gccataactg
    11281 aaaaaggagt ctatgaccgt ggtgcatcat ttattcagat gggattgatt gtaactacaa
    11341 catttagttt tgcgctcatt cctctgttaa gtgacgcaat caaaatgaat aatcaggtac
    11401 ttatgaatcg ttatgcaaat gcgtcattaa agattacgat tttaataagt acagcagcgg
    11461 gaataggatt aattaattta ttgcctttaa tgaacggtgt gttttttaag acgaatgatt
    11521 taaccttaac gttaagtgtt tatatgatta cggtcatttg tgtatcgtta attatgatgg
    11581 atatggcttt attacaagcg caacatgctg tgagacctat ttttgttggt atgacggcag
    11641 gattggttat taaatttata cttaatatca ttttgattcg tttaagtggc attattggtg
    11701 cgagcattag tactgttgta tcattaatta tattcggtac gattatccat attgctgtca
    11761 cgagaaaata ccacttacat gcgatgagac gattttttat caatgttgtt ttaggtatgg
    11821 tatttatgtc gattgttgtt caatgcgtgt taaacatagt gacaacacac ggtagattca
    11881 ctggactcat tgaattatta tgtgcagcag tattaggtat cattgcattg tttttctata
    11941 tttttagatt taatgttttg acatataaag agttaactta tttaccattt ggttcaaagt
    12001 tgtatcaaat taagaaagga agacgttgat ggcacatacc attacgattg ttggcttagg
    12061 aaactatggc attgatgatt tgccgctagg gatatataaa tttttaaaga cacaagataa
    12121 agtttatgca agaacgttag atcatccagt tatagaatca ttgcaagatg aattaacatt
    12181 tcagagtttt gaccatgttt atgaagcaca tgaccaattt gaagatgtct ataacgatat
    12241 tgtggcgcaa ttggttgaag ctgctaatga aaaagatatt gtctatgcgg ttccgggtca
    12301 tcctagagtt gctgagacaa ctacagtgaa attactggct ttagcaaagg acaatactga
    12361 tatagatgtg aaagttttag gtggtaaaag ctttattgat gatgtgtttg aagcagttaa
    12421 tgtagatcca aatgatggct tcacactgtt agatgcgaca tcattacaag aagtaatact
    12481 taatgttaga acgcatacat tgattacgca agtttatagt gcaatggttg ctgctaattt
    12541 gaaaatcact ttaatggaac gatatcctga tgattaccct gttcaaattg tcactggtgc
    12601 acgaagcgat ggtgcggata acgttgtgac atgcccatta tatgaattgg atcatgatga
    12661 aaatgcattc aataatttga cgagtgtatt cgtaccaaaa atcataacat cgacatattt
    12721 gtatcatgac tttgattttg caacggaagt gattgatact ttagttgatg aagataaagg
    12781 ttgtccatgg gataaagtgc aaacgcatga aacgcttaag cgttatttac ttgaagaaac
    12841 atttgaattg ttcgaagcta ttgacaatga agatgattgg catatgattg aagaactagg
    12901 agatatttta ttacaagtgt tattgcatac tagtattggt aaaaaagaag ggtatatcga
    12961 cattaaagaa gtgattacaa gtcttaatgc taaaatgatt cgtagacacc cacacatatt
    13021 tggtgatgcc aatgctgaaa ctatcgatga cttaaaagaa atttggtcta aggcgaaaga
    13081 tgctgaaggt aaacagccaa gagttaaatt tgaaaaagta tttgcagagc attttttaaa
    13141 tttatatgag aagacgaagg ataagtcatt tgatgaggcc tcgttaaagc agtggctaga
    13201 aaaaggggag agtaatacat gagattagat aaatatttaa aagtatcacg gttaataaag
    13261 cgacgtacgc tagcaaaaga agtaagtgat caaggtagaa ttacaataaa tggtaatgtt
    13321 gctaaagctg gatcggatgt taaagttgaa gatgtgctga cgattcgctt tggtcaaaaa
    13381 ttagtaacag ttaaagtaac tgcattaaat gaacatgcat ctaaagataa cgcgaagggc
    13441 atgtatgaaa tcattgaaga gcgtcgactt gaagaagcgt aaattggagg tgacaagcaa
    13501 tgaaaaataa agtagaacat atagaaaatc agtacacgtc gcaagagaac aagaaaaaac
    13561 aacgtcaaaa aatgaaaatg cgtgttgttc gtaggcgtat tacagtattt gcgggcgtat
    13621 tacttgcgat aattgttgtt ttatcaatct tgcttgttgt ccaaaaacat cgcaatgata
    13681 ttgatgcaca ggagcgaaaa gcgaaagaag cacagtttca aaagcaacaa aatgaagaaa
    13741 ttgcgttaaa agaaaagttg aataatctga atgacaaaga ttacattgaa aaaattgcgc
    13801 gtgatgatta ttacttaagc aacaaaggtg aagtgatttt taggttgcca gaagacaaag
    13861 attcgtctag ctcaaaatct tcgaaaaaat aaatccaaat tgattcaaaa ttatccgagt
    13921 atagacattg tgaaaaaatc caaacaagga tataataagg gaaaatcgaa tcaaatcggg
    13981 aggatttatt taacatatgt caatcgaagt tggaaataag cttaaaggta aagtcactgg
    14041 tattaaaaag tttggtgcat tcgtagaatt acctgaagga aaaagtggtt tagttcacat
    14101 tagtgaagtc gcagataatt atgttgaaaa cgtagaagag cacctttctg ttggtgatga
    14161 agtagacgtg aaagtattat ctattgctga tgatggaaaa attagtcttt caattaagaa
    14221 agctaaagac cgtccacgta gacaacatac gagtaaacca agtcatcaaa aaccagtgca
    14281 aaaagccgaa gattttgaaa agaaattaag caatttctta aaagatagtg aagataaatt
    14341 aacttcaatc aaacgtcaaa cagaatctag acgcggtggc aaaggttcta gacgttaatt
    14401 aaaataaata aagactgttt cgataaggaa tatatttaga atgatgcgta tcgaataatc
    14461 gattgcagcg ttagacaatc taagactgtt tcttaaataa ggagcagtct cttttatttg
    14521 taatgatata actaagactt ataccatttt tgaaaattgt aaaagtgagg tgatgttatg
    14581 cagttaaata gtaatggttg gcatgttgat gaccatattg ttgtcgctgt ttctacaggt
    14641 attgatagta tgtgtttatt gtatcaacta ctaaatgatt ataaagatag ttatagaaaa
    14701 ctaacatgct tacatgtcaa tcatggcgtt aggtcagctt caattgagga agccagattt
    14761 ttagaagcat actgcgaacg tcatcacatt gatttacata tcaaaaagtt agatttgtcg
    14821 catagtctcg accgaaataa cagcattcag aatgaagctc gaattaaacg ttacgaatgg
    14881 tttgatgaaa tgatgaatgt attagaagcg gatgtattgc taacggcgca tcatttggac
    14941 gatcaattag aaactattat gtatcgtatt tttaatggga aatcaacgcg taataaacta
    15001 ggatttgatg agttatcgaa gcgaaaaggt tatcagattt atcgaccact tttagctgtc
    15061 tctaaaaaag aaataaaaca attccaagag agatatcata ttccatattt tgaagatgaa
    15121 tctaataaag ataacaaata tattagaaat gatattcgta atagaattat tccagctatt
    15181 gatgaaaata atcaacttaa agtatcgcat ttattaaaat taaaacaatg gcatgatgaa
    15241 caatatgata ttttgcaata ttcagctaaa caatttattc aagaatttgt gaagtttgat
    15301 gaacagtcaa aatatttaga ggtttctaga caagctttta ataacttacc aaactcatta
    15361 aagatggttg tgttggactg cctattatca aagtattatg agttgtttaa tattagtgct
    15421 aaaacatacg aagagtggtt taaacaattt agtagtaaga aagcacaatt cagtattaat
    15481 ctcacggata aatggataat tcaaatcgca tatggtaaat taataataat ggctaaaaat
    15541 aatggcgata catattttag agttcaaaca attaaaaagc caggtaatta tttttttaac
    15601 aaatatcgat tagagataca ttctaattta ccaaaatgtt tatttccgct tacagtgaga
    15661 acacgacaaa gtggcgatac atttaaactg aatgggcgcg atggttataa gaaagtgaat
    15721 cgcctgttta tagattgtaa agtgccacag tgggttcggg atcaaatgcc aatcgtattg
    15781 gataaacaac agcgcattat tgcggtagga gatttatatc aacaacaaac aataaaaaaa
    15841 tggattataa ttagtaaaaa tggagatgaa tagcgttatg cataatgatt tgaaagaagt
    15901 attgttaact gaagaagata ttcaaaatat ctgtaaggaa ttgggagcac aattaacaaa
    15961 ggattatcaa ggtaaaccat tagtatgcgt gggtatctta aaaggctcag caatgtttat
    16021 gtcagattta attaaacgaa ttgataccca tttatcaatt gatttcatgg atgtttctag
    16081 ttatcacgga ggcactgagt caactggtga agttcaaatc attaaagatt taggttcttc
    16141 tattgaaaat aaagacgtat taattattga agatatctta gagactggta ctacacttaa
    16201 gtcaattact gaattattac aatctagaaa agttaattca ttagaaatag ttactttatt
    16261 agataaacca aaccgtcgta aagcggacat tgaagctaag tatgtaggta aaaaaatacc
    16321 agatgaattt gttgttggtt acggtttaga ttatcgtgaa ttataccgaa acttaccata
    16381 tatcggtacg ttaaaacctg aagtgtattc aaattaattt tttaatcaat ttcagttatt
    16441 attactatgc gtttgagaaa taatagtgta gac
//
LOCUS       DANBWH010000039        16186 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.041, whole genome shotgun sequence.
ACCESSION   DANBWH010000039 DANBWH010000000
VERSION     DANBWH010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16186
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.041"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(356..1216)
                     /locus_tag="PPY92_000892"
     CDS             complement(356..1216)
                     /locus_tag="PPY92_000892"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002468309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HDJ2624911.1"
                     /translation="MPLVSMKEMLIDAKENGYAVGQYNINNLEFTQAILEASQEENAP
                     VILGVSEGAARYMSGFYTIVKMVEGLMHDLNITIPVAIHLDHGSSFEKCKEAIDAGFT
                     SVMIDASHSPFEENVATTKKVVEYAHEKGVSVEAELGTVGGQEDDVVADGIIYADPKE
                     CQELVEKTGIDALAPALGSVHGPYKGEPKLGFKEMEEIGLSTGLPLVLHGGTGIPTKD
                     IQKAIPFGTAKINVNTENQIASAKAVRDVLNNDKEVYDPRKYLGPAREAIKETVKGKI
                     KEFGTSNRAK"
     gene            1434..1955
                     /locus_tag="PPY92_000893"
     CDS             1434..1955
                     /locus_tag="PPY92_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2529 domain-containing protein"
                     /protein_id="HDJ2624912.1"
                     /translation="MSKILNTQLIGIFNRLEKQSLEIQMAAQCLIQAIGGEGYVYVKG
                     YDDLQFFESFILHSDERLKSSRKLDAIKDFKEIDSTDRVLLFAPFYNDQVALDIQKLI
                     DLDIDVVLISNKPKTDDFPDHLVHFIDLSTPRPIVYTEDYDKIVQPHAIALNYVYYDI
                     YTQMIEMTRDLEL"
     gene            complement(2064..3674)
                     /locus_tag="PPY92_000894"
     CDS             complement(2064..3674)
                     /locus_tag="PPY92_000894"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HDJ2624913.1"
                     /translation="MTKFIFVTGGVVSSLGKGITASSLGRLLKDRGLNVTIQKFDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKFSNVTAGKVYSHVLKKE
                     RRGDYLGGTVQVIPHITNEIKERLLLAGESTNADVVITEIGGTTGDIESLPFIEAIRQ
                     IRSDLGRENVMYVHCTLLPYIKAAGEMKTKPTQHSVKELRGLGIQPDLIVVRTEYEMT
                     QDLKDKIALFCDINKESVIECRDADSLYEIPLQLSQQNMDDIVIKRLQLNAKYETQLD
                     EWKQLLDIVNNLDGKITIGLVGKYVSLQDAYLSVVESLKHAGYPFAKDIDIRWIDSSE
                     VTDENAAEYLADVDGILVPGGFGFRASEGKISAIKYARENNVPFFGICLGMQLATVEF
                     SRNVLGLEGAHSAELDPATPYPIIDLLPEQKDIEDLGGTLRLGLYPCSIKEGTLAQDV
                     YGKAEIEERHRHRYEFNNDYREQLEANGMVISGTSPDGRLVEMVEIPTNDFFIACQFH
                     PEFLSRPNRPHPIFKSFIEASLKYQQNK"
     gene            complement(4010..4540)
                     /locus_tag="PPY92_000895"
     CDS             complement(4010..4540)
                     /locus_tag="PPY92_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HDJ2624914.1"
                     /translation="MKIQDYTKQMVDEKSFIDMAYTLLNDKGETMNLYDIIDEFRALG
                     DYEYEEIENRVVQFYTDLNTDGRFLNVGENLWGLRDWYSVDDIEEKIAPTIQKFDILD
                     ADDEEDQNLKLLGEDEMDDDDDIPAQTDDQEELNDPEDEQVEEEINHSDIVIEEDEDE
                     LDEDEEVFEDEEDFND"
     gene            complement(4652..5512)
                     /locus_tag="PPY92_000896"
     CDS             complement(4652..5512)
                     /locus_tag="PPY92_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDJ2624915.1"
                     /translation="MAKELCFEGITLKAFDEQYRSAINDFDLNERQQIYSSLPKEVID
                     DAINDVDRIANVAINDKNEVVGFFVLHRYYQHEGYDTPENVVYIRSLSINEKYQGFGY
                     GTKIMMSLPQYVQGVFPDFNHLYLVVDAENDNAWNLYERAGFMHTATKEEGPIGKERL
                     YYLDLDSKHVSSLKLEEESRSEVTNVHIINLMIDGQKVGFIALEQIGERMNIAAIEVD
                     KSYRFNGIGSSALRQLPTYLRKNYDNLNVITMILFGENNDFKPLCLNSNFVEIEQTDD
                     YVVFEKYLNY"
     gene            5937..6740
                     /gene="coaW"
                     /locus_tag="PPY92_000897"
     CDS             5937..6740
                     /gene="coaW"
                     /locus_tag="PPY92_000897"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II pantothenate kinase"
                     /protein_id="HDJ2624916.1"
                     /translation="MKVGIDAGGTLIKIVQEQDNQRTFKTELTKNIDQVVEWLNQQQI
                     EKLCLTGGNAGVIAENINIPAQIFVEFDAASQGLGILLKEQGHDLADYIFANVGTGTS
                     LHYFDGQSQRRVGGIGTGGGMIQGLGYLLSQITDYKQLTDMAQHGDRNTIDLKVRHIY
                     KDTEPPIPGDLTAANFGHVLHHLDADFTPSNKLAAVIGVVGEVVTTMAITVAREFKTE
                     NIVYIGSSFHNNALLRKVVEDYTVLRGCKPYYVENGAFSGAIGALYLGK"
     gene            complement(6871..7542)
                     /locus_tag="PPY92_000898"
     CDS             complement(6871..7542)
                     /locus_tag="PPY92_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2750 domain-containing protein"
                     /protein_id="HDJ2624917.1"
                     /translation="MSYKAYPFFRDILINECIYFASKNKKLVRLNYKSEAYVGVWTEE
                     SVAVSFLTSRDIPFDKVVKMDVDRFATYELDELFDEQDHIIMNQTMEEEGHLLNVVAV
                     TQEVMTELDKIRIKEFVQDVAKYDEVYGLTKKGSKQFILISENDSDEKKPHIMPVWSI
                     KNRALKVRDEDFEECDLITIEGSVFGEWLDELRDDHKAVAIDLKTGVVGTIVSAQKLS
                     NELTF"
     gene            complement(7941..9134)
                     /locus_tag="PPY92_000899"
     CDS             complement(7941..9134)
                     /locus_tag="PPY92_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDJ2624918.1"
                     /translation="MTNNGNEPNLTLSDLYDKDVVYTSRPSYISNPWLKPDEHQSNFL
                     TGRELLIANQLPVIVHEASATDKLHQLFQVIGKEVPNSIFTFNNQQSYENLIKQLAHK
                     ENKKIYFQYIHDETILNQQYYALDKTLFVALNNKARIPEWTNGKFLPKRKVVKIEQFE
                     NEIKNWEFPLVIKPGDDLPTAGGYGVMICYHDADLQKAITRIKEATAETNSLIIEQKI
                     EEKANYCVQFAYSESLGIQYLGAATQLTDKYGFYNGNENTTNVPEHVIEAGRQIMENG
                     VNQGFFGVAGFDLLVDEDDNVYAIDLNFRQNGSTSMLLLANELNSGYQKFYSYHSKGD
                     NTHFFNTILKYVKEGSLYPLSYYDGDWYGEDKVKSRFGCIWHGDSKETVLENERAFLA
                     ELEHY"
     gene            complement(9134..10318)
                     /locus_tag="PPY92_000900"
     CDS             complement(9134..10318)
                     /locus_tag="PPY92_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDJ2624919.1"
                     /translation="MSEKQQILDYIETNKYSYIEISHRIHERPELGNEEIFASRTLID
                     RLKEHDFEIETEIAGHATGFIATYDSGLDGPAIGFLAEYDALPGLGHACGHNIIGTAS
                     VLGAIGLKQVIDQIGGKVVVLGCPAEEGGENGSAKASYVKAGVIDQIDIALMIHPGNE
                     TYKTIDTLAVDVLDVKFYGKSAHASENADEALNALDAMISYFNGVAQLRQHIKKDQRV
                     HGVILDGGKAANIIPDYTHARFYTRAMTRKELDILTEKVNQIARGAAIQTGCDYEFGP
                     IQNGVNEFIKTPKLDDLFAKYAEEVGEAVIDDDFGYGSTDTGNVSHVVPTIHPHIKIG
                     SRNLVGHTHRFREAAASVHGDEALIKGAKIMALMGLELITNQDVYQDIIEEHAHLKGN
                     GK"
     gene            10629..11099
                     /locus_tag="PPY92_000901"
     CDS             10629..11099
                     /locus_tag="PPY92_000901"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDJ2624920.1"
                     /translation="MTKMNVESFNLDHTKVVAPFIRLAGTMEGLNGDVIHKYDIRFKQ
                     PNKEHMDMPGLHSLEHLMAENIRNHSDKVVDLSPMGCQTGFYVSFINHDNYDDVLNIV
                     EATLNDVLNATEVPACNEVQCGWAASHSLEGAKTIAQAFLDKRNEWHDVFGTGK"
     gene            complement(11251..11601)
                     /locus_tag="PPY92_000902"
     CDS             complement(11251..11601)
                     /locus_tag="PPY92_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624921.1"
                     /translation="MNILRYLLGIGFSVIGVLHFTRERQFRNIIPKCLPLRKTAVLVT
                     GIFEILFGLALLIKRPSQCLKNTINLFLLAVFPANIYMAVKKLPLGDKQLPTWQLYLR
                     LPMQFVLMGLIKKL"
     gene            complement(11615..12916)
                     /locus_tag="PPY92_000903"
     CDS             complement(11615..12916)
                     /locus_tag="PPY92_000903"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDJ2624922.1"
                     /translation="MRMIDIIEKKRDGHTLTTEEINFFIDGYVKGDIPDYQASSLAMA
                     IYFQDMNDDERAALTMAMVNSGDMIDLSDIKGVKVDKHSTGGVGDTTTLVLAPLVAAV
                     DVPVAKMSGRGLGHTGGTIDKLEAIDGFHVEIDEATFVKLVNENKVAVVGQSGNLTPA
                     DKKLYALRDVTGTVNSIPLIASSIMSKKIAAGADAIVLDVKTGSGAFMKTLEDAEALA
                     HAMVRIGNNVGRNTMAIISDMNQPLGRAIGNALELQEAIDTLKGQGPKDLTELVLTLG
                     SQMVVLANKAETLEEARALLIEAINSGAALEKFKTFIKNQGGDETVIDHPERLPQAQY
                     QIEYKAKKSGYVTELVSNDIGVASMMLGAGRLTKEDDIDLAVGIVLNKKIGDKVEEGE
                     SLLTIHSNRQDVDDVVKKLDSSITIADHVVSPTLIHKIITE"
     gene            complement(13195..13857)
                     /gene="deoC"
                     /locus_tag="PPY92_000904"
     CDS             complement(13195..13857)
                     /gene="deoC"
                     /locus_tag="PPY92_000904"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDJ2624923.1"
                     /translation="MNSAKLIDHTLLKPESTRTQIDQIIDEAKAYHFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVVKAAKGHTVKVIIETVLLDHDEIVKASELTKVAGADFVKTSTGFAGGG
                     ATAEDVKLMKDTVGADVEVKASGGVRNLEDFNKMVEAGATRIGASAGVQIMQGLEADS
                     DY"
     gene            14171..14881
                     /gene="deoD"
                     /locus_tag="PPY92_000905"
     CDS             14171..14881
                     /gene="deoD"
                     /locus_tag="PPY92_000905"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDJ2624924.1"
                     /translation="MTKGTPHIQPNGVKIAKTVLMPGDPLRAKYIADNFLENVEQFND
                     VRNMFGYTGTYKGKEVSVMGSGMGIPSIGIYSYELYNFFDVDTIIRIGSCGALQENVN
                     LYDVIIAQAASTNSNYVDQYNIPGHFAPIADFELVTKAKNVADQIGATTHVGNVLSSD
                     TFYNADPTFNDAWKKMGILGIEMESAGLYLNAIHAGKKALGIFTVSDHILRDEATTPE
                     ERQNSFTQMMEIALEIAE"
     gene            complement(15002..15445)
                     /locus_tag="PPY92_000906"
     CDS             complement(15002..15445)
                     /locus_tag="PPY92_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDJ2624925.1"
                     /translation="MSNQQDVVKELNQQVANWTVAYTKLHNFHWYVKGPNFFSLHVKF
                     EELYNEASQYVDELAERILAVGGNPVGTLTECLEQSIVKEAAKGYSAEQMVEELSQDF
                     TNISKQLENAIEIAGNAGDDVSEDMFIGMQTSVDKHNWMFKSYLS"
     gene            complement(15645..16058)
                     /locus_tag="PPY92_000907"
     CDS             complement(15645..16058)
                     /locus_tag="PPY92_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HDJ2624926.1"
                     /translation="MPIVYYDGNCIYCYNYVIWLIQHDLPRNYQFATLKGEVGQQFFK
                     QHPEAANKNSVILQKGDRIYFESQAIIKLITALPNRTKLLGVGLWLVPKPVRNFGYRM
                     FANNRNRMWKTTWHQPNDYEKSFFLDDNAKVKLTD"
BASE COUNT         5093 a         3083 c         2258 g         5752 t
ORIGIN      
        1 ttctattttc gattgattaa taacaaatca ttcaccttta ctaatatatg ctgttcttcc
       61 ccacttgacc atggtctaaa gtctgattat tcaactaaat attgacgttg ttttgataat
      121 tatttagttt taattctacg atttgtacgt aaactttttt cataaattgc tcattttcct
      181 tataattata tcacaatcaa ttttggtata ttttaccatt tttacgcttt cattttaaaa
      241 tcaaactgtt ttatttatta cttatacgac ataaaaaaga agcaaacacc attttgtctg
      301 cttcttttaa aattaatatc cctacgttat taataactta aaaactaaat attaattatt
      361 tagcgcggtt tgaagtaccg aactctttaa ttttaccttt aactgtttct ttgatggctt
      421 cacgtgcagg tcctaagtat ttacgaggat cgtaaacttc tttgtcgtta tttaaaacgt
      481 cacgaactgc ttttgctgaa gcgatttggt tttcagtgtt tacgttaatt ttagctgtac
      541 caaatggaat tgctttttgg atatctttag tcgggatacc agtaccaccg tgtaatacta
      601 atggtaaacc tgtagataaa ccgatttctt ccatttcttt aaatcctaat tttggttcac
      661 ctttgtatgg accatgaact gaacctaatg ctggcgctaa tgcatcaata ccagtttttt
      721 caactagttc ttgacattct ttaggatcag cataaatgat gccgtctgct acaacatcat
      781 cttcttgtcc accaacagta cctaattcag cttctacaga aacacctttt tcatgagcgt
      841 attcaacaac ttttttagtt gttgctacgt tttcttcgaa tgggctgtgt gaagcatcga
      901 tcattactga tgtgaaacca gcatcgatag cttctttaca tttttcaaag cttgaaccat
      961 ggtctaaatg gattgctaca ggaatagtga tgtttaagtc atgcattaac ccttcaacca
     1021 ttttaacaat tgtgtagaaa ccgctcatgt aacgagcagc accttcagaa acacctaaaa
     1081 ttacaggtgc attttcttct tgtgacgctt ctaaaattgc ttgagtgaat tctaggttat
     1141 taatattgta ttgacctacc gcataaccat tttcttttgc atcaattaac atttctttca
     1201 ttgaaactaa aggcatgaaa gttcctcctt gagtgcttac gcacgtattt ttcaaagtaa
     1261 ttatatcaaa aaaatagcgt caatgcattg aaatactgca gattaaatgt gtattttttg
     1321 aaaaatgcga tagttatact aaatgcaaac gtatacattt ttatacaatt taagccgttg
     1381 cataatatgt agtcattaat tacaatattt tataagtaga ttggaggata aagatgtcaa
     1441 aaatcttaaa cacacaacta ataggtatct ttaataggct agaaaaacaa tctttagaaa
     1501 ttcaaatggc cgcccaatgt ttaattcaag caattggtgg cgaaggttat gtttatgtaa
     1561 aaggttatga tgatttacag tttttcgaaa gtttcattct acatagtgat gagcgtttaa
     1621 aatctagccg aaaacttgac gctattaaag actttaaaga aattgactca actgatcgtg
     1681 tattattatt cgcaccattt tataatgatc aagttgcatt agatattcaa aaacttatcg
     1741 acttagatat cgatgtggtc ttaataagca acaaacctaa aacagatgat ttccctgatc
     1801 atctcgtaca ttttatcgat ttatctacac caagacctat agtctatact gaagattatg
     1861 ataagattgt ccagccacat gcgatagcat tgaattatgt atactatgac atttatacac
     1921 aaatgattga aatgacaaga gacttagaat tataaaagtc actattgtta tttcatacct
     1981 tgtcactaac ataatattaa aattatacga aaaaccggta ctttcaatgt ttaacaatga
     2041 aagtaccggt tttattagca aatttattta ttttgttgat attttaatga agcttcaata
     2101 aatgatttaa aaatcgggtg cggacgattt ggtctagata agaattctgg gtggaattga
     2161 caagcaataa agaaatcatt tgtcggaatc tctaccattt ctactaaacg tccatctgga
     2221 cttgtaccag aaattaccat accatttgct tctaattgtt ctctatagtc attattaaat
     2281 tcataacgat gacgatgtct ttcttcaatt tccgctttac cataaacatc ttgtgccaat
     2341 gtgccttctt taatagaaca tggatataag cctaagcgta atgtaccacc taaatcttcg
     2401 atatcttttt gttctggtaa taaatctata attgggtatg gtgttgctgg gtctaattca
     2461 gctgaatgtg cgccttcaag tcctaatacg tttcttgaaa attcaactgt tgcaagttgc
     2521 attcctaaac aaataccaaa gaatggtaca ttgttttctc tagcatactt aattgcacta
     2581 attttacctt cacttgcacg gaaaccaaat ccacctggta ctaaaatacc gtcgacatct
     2641 gcaaggtatt cggctgcatt ttcatctgtt acttcacttg aatcaatcca tctaatgtca
     2701 atatctttgg caaaaggata tccagcatgt ttcaatgatt caacaactga taaatatgca
     2761 tcttgtaagc taacatattt acctactaaa ccaattgtaa ttttaccatc taaattatta
     2821 acgatatcta acaactgttt ccattcatca agctgtgttt catatttcgc gtttaattgt
     2881 aaacgtttaa taacgatatc atccatattt tgttggctta attgtaatgg aatttcgtat
     2941 aaagagtctg catcacgaca ttcaataaca ctttctttat taatgtcaca gaataatgca
     3001 attttatctt ttaaatcttg tgtcatttca tattcagttc ttacaacgat taagtctggt
     3061 tgaataccta agcctcgtaa ttctttaaca ctatgttgtg ttggcttcgt tttcatttct
     3121 ccagcagctt taatataagg cagtaatgta cagtgaacat acataacatt ttctctacct
     3181 aaatcgctac gaatttgacg aatcgcttca ataaacggta atgactcaat atcacctgtt
     3241 gtaccgccaa tttcagtgat aacaacgtct gcattcgtac tttcccctgc aagtaataaa
     3301 cgttctttaa tttcatttgt aatatgcgga ataacttgaa ctgttccgcc taagtaatca
     3361 ccacgacgtt cttttttcaa tacgtgtgaa tacactttac cggctgtcac atttgaaaac
     3421 ttgtttaaat taatatcaat aaatctttcg taatgtccta agtctaggtc agtttctgca
     3481 ccatcatccg ttacgaatac ttcaccatgt tgataaggac tcattgtacc tgggtcaaca
     3541 tttaagtatg gatcgaattt ttgaattgtt acatttagac ctctatcttt taataatcta
     3601 cctagagaag atgctgtaat acccttccct aatgatgaaa ctacgccacc tgttacaaaa
     3661 ataaattttg tcatgtcttg acctcctaat tattaaaggg aatgcttcct catcaattgt
     3721 aaaaattacg taaaacataa cattaaagca agctcattca agttgtttat ttgtcgaatt
     3781 cgacaaaatt aaattctgtt tacttaccgt ttccatactg ctatgtcact atgtatatca
     3841 tttacaactt tgatttttgc gttatcattt cttgatttac tcaattaaat aaaaaaacgc
     3901 tccctctcac aaattctgta aggggagcgt ataaatttta tacacgtgtc ctatttaaag
     3961 gagcccgaat aaaattttat catctttcaa ctaaaagtca aacaaaaaat taatcgttga
     4021 agtcttcttc gtcttcaaac acttcttcgt cttcgtctag ttcatcttca tcttcttcaa
     4081 tgactatatc cgaatgattg atttcttctt caacctgctc atcttctgga tcatttagtt
     4141 cttcttgatc atctgtttga gctggaatat catcgtcgtc atccatttca tcttcgccca
     4201 ataattttaa gttttgatct tcttcatcat ctgcatccag aatatcgaat ttttgaatag
     4261 ttggtgcgat tttctcttca atatcatcta ccgaatacca atcacgtaat ccccataaat
     4321 tttctccaac atttaaaaaa cgaccatctg tgtttaaatc tgtgtaaaat tgtacaacgc
     4381 gattttcaat ttcttcgtac tcataatcac ctaacgctct aaactcatcg atgatatcat
     4441 ataagttcat agtttcgcct ttatcattca ataatgtata agccatatca ataaatgatt
     4501 tttcatcaac catttgtttt gtataatctt gaattttcat tgcccaatac ttcctttcga
     4561 aggatataat ctaaatttat acgtctgaat tcatttatat atagtaacaa aactaaatta
     4621 taaatgacaa tatcatattt cgcaatcact gttagtaatt taaatatttt tcaaaaacga
     4681 cataatcatc agtttgttcg atttcaacga aattactatt taaacataat ggtttaaaat
     4741 cattattctc tccaaacaga atcatcgtaa tcacattaag gttgtcatag ttttttctta
     4801 agtaagttgg caattgtcgc agcgcacttg aaccaatacc attaaagcga tatgatttat
     4861 ccacttcaat agcagcaatg ttcatgcgtt caccaatctg ctccaatgcg ataaagccaa
     4921 ccttttggcc atcaatcatt aaattaatga tatgtacatt ggtcacttct gaacgacttt
     4981 cttcttcaag ctttaatgac gaaacatgtt ttgaatctaa gtccaagtaa tatagtcgtt
     5041 ctttgccaat tggtccttct tctttagtcg ctgtatgcat gaatccagca cgttcgtata
     5101 ggttccaagc attgtcattt tccgcatcta ctactagata tagatgatta aaatcaggaa
     5161 atacaccttg aacatattgc ggcaatgaca tcattatttt cgtgccatat ccaaaacctt
     5221 gatatttttc attaatcgat aatgaacgaa tataaacgac attttcaggt gtatcataac
     5281 cttcatgctg atagtaacga tgtaatacaa aaaagcccac cacttcattt ttatcgttta
     5341 ttgctacgtt agcaatccta tcaacatcat taattgcatc atcaataact tctttaggta
     5401 aagatgaata tatttgttgt ctttcattca agtcaaaatc attaattgct gaacgatatt
     5461 gttcatcaaa tgcttttaaa gtgatacctt caaaacaaag ttctttagcc atatatttaa
     5521 cccctatctt tttatatatg aagcatgaat ccccatatta ttttacctat tatttcacta
     5581 cgataccatt atttactcta agttaaataa attacttatt tatatttttt taaatttcaa
     5641 aattaatgtg ctattcctca ttactcatca ttttaatcag agacttacaa atacactgcg
     5701 tacgaactac ttttaatatt gcgtcactaa ctaacaatcc tatacaactt caaaattcct
     5761 agaaaataat atatataact taacgtctac gtttgtaata cattaattgc gatgcgctta
     5821 caatatcgaa atacaatggc atcaaatttg ttaataatta ctattattac ttgtttttta
     5881 gtcattttac tatactatta tttaaggata gacattaata atgaggtggg aatcttatga
     5941 aagttggcat tgacgctggc ggtacactaa ttaaaatcgt tcaagagcaa gataaccaac
     6001 gtacttttaa aactgaatta actaaaaata ttgatcaagt tgtggaatgg ttaaaccaac
     6061 agcaaattga aaaattatgc ttaactggag gtaatgcagg tgtcattgct gaaaacatca
     6121 acattcctgc acaaattttt gtagagtttg atgctgcatc tcaaggacta ggtattttgt
     6181 taaaagagca aggccatgac ttagcagatt atatttttgc aaatgttggt accggtacgt
     6241 cactacatta tttcgacggc caatcacaac gtcgtgtagg cggtatcggt acaggtggag
     6301 gcatgattca aggactcggt tatttactaa gtcaaatcac tgattataaa cagttaactg
     6361 acatggcaca acacggtgat cgtaacacta tcgatttaaa ggttagacat atttataaag
     6421 ataccgaacc acccattccc ggtgatttaa cagcagctaa ttttggtcat gtactacatc
     6481 atttagatgc tgattttaca cctagcaata aattagcagc cgttattggt gtcgttggcg
     6541 aagttgttac gacaatggcc attactgttg cacgtgaatt taaaactgaa aatatcgttt
     6601 atatcggttc ttcattccat aataacgctt tattacgcaa agttgtggaa gattatactg
     6661 tgttacgcgg ttgcaaaccg tactatgttg aaaacggcgc attttcaggt gctattggtg
     6721 cattatattt aggtaaatag aaaaagacca tttatatgcg cgttgtttta aataaacgct
     6781 atataaatgg ccttttattt tatggtctac gtgttttgga atacgtattg ttccatttat
     6841 tatcacttaa ttacttacaa ctcaattcaa ttaaaatgta agttcattag acaatttttg
     6901 cgctgaaaca attgtaccaa ccacgccagt ttttaaatct attgcaacgg ctttatgatc
     6961 atctctaagt tcatctagcc attctccgaa aacagaacct tcaatcgtaa ttaaatcaca
     7021 ctcttcaaaa tcttcatctc gaacttttaa cgctctgttt ttaatactcc atacaggcat
     7081 aatatgcggc tttttttcgt cgctatcatt ttcactaatg agaataaact gcttactacc
     7141 ttttttagtt aagccgtata cttcatcata ttttgctaca tcttggacaa attctttgat
     7201 tctaatttta tctaattccg tcatcacttc ttgtgtaaca gctacaacgt ttagtagatg
     7261 cccttcttct tccattgttt gattcataat aatatggtct tgttcatcaa acaattcatc
     7321 taattcataa gtagcaaaac gatcaacgtc catttttaca actttatcaa atggaatatc
     7381 acgacttgtt aaaaatgata cggccacact ttcttctgtc caaacgccta catacgcttc
     7441 acttttataa tttaggcgta ctagtttttt atttttagag gcgaaataaa tacattcatt
     7501 tattaatata tctctaaaga atggatatgc tttataagac atgcttgacc ctcacttttt
     7561 aaaatatgtc atacataaaa tagtaaatga ctccaacaag ttagtcaatt agacatatta
     7621 tgaattcaat ttaatgtcat gtttttcatt ttaaaaattc aatagcatta aattttacgt
     7681 atacacaaat tcggtctata acacgaaaca tatctagtta ctaaaaattt caaaacgata
     7741 acctttagta tacctttatc aacaaaatgg caactcttat aacatagtga aataaaagtc
     7801 gccatttaat attaatatta caaagtattg ccatatcata aatttaatta ctgttgacta
     7861 gaatcgaaaa aagcttgtta caaacgcata ttcagtcaac tactggcaac acaacattgt
     7921 agcgccttat gttccgaact ctaatagtgt tcaagttcag ctaaaaatgc gcgttcattc
     7981 tccagtactg tttcttttga atcaccatgc caaatacagc caaaccttga tttaacttta
     8041 tcttcaccgt accaatcacc atcataataa gataacgggt ataaactacc ttctttgaca
     8101 tatttcaaaa tcgtattgaa gaaatgtgtg ttatcacctt ttgaatgata actataaaac
     8161 ttttgatatc ctgaattcaa ctcgttagca agtaataaca tgctcgttga accattttgt
     8221 ctaaagttta aatcaatcgc ataaacgtta tcatcctcat cgacgagtag gtcaaaacca
     8281 gctacaccaa agaatccttg attcacaccg ttttccataa tttgtctacc tgcttctata
     8341 acgtgctctg gtacattagt agtattttca tttccgttat aaaatccgta cttatccgtt
     8401 aattgcgttg ccgcaccaag atattgaatg cctagcgatt ccgagtatgc aaactgaacg
     8461 caataattcg ctttttcttc aatcttctgt tcgataataa gcgaattcgt ttcagcagtt
     8521 gcctctttaa tacgcgttat cgctttttgt aaatctgcat catgataaca aatcataacg
     8581 ccatagccac ctgctgtcgg aagatcatct cccggcttaa ttactaacgg gaattcccaa
     8641 ttcttaatct cgttttcgaa ttgctcaatt tttacaactt ttctttttgg taaaaacttc
     8701 ccatttgtcc attcaggtat tcttgcttta ttatttaaag caacaaataa cgttttatct
     8761 aatgcataat attgctgatt caagattgtt tcatcatgaa tatattgaaa ataaatcttt
     8821 ttattttcct tatgtgccaa ttgtttgatc aagttttcgt aagattgctg attgttaaat
     8881 gtaaaaattg agttcggtac ttccttacca ataacttgaa atagctgatg caatttgtct
     8941 gtcgcactag cttcgtgaac aataacaggt aattgatttg ctattaataa ctccctacca
     9001 gttaaaaaat tagattgatg ttcgtccggt ttcaaccatg gattcgatat atacgaaggt
     9061 cttgacgtat agacaacatc tttgtcatat aaatcactta acgttaagtt cggctcatta
     9121 ccattatttg tcattacttc ccattccctt tcaaatgcgc atgctcttca ataatgtctt
     9181 gataaacgtc ttgatttgta attaactcta accccatcaa cgccattatt ttagcacctt
     9241 taattaatgc ttcatcacca tgtacactcg cagccgcctc tctaaatcta tgcgtatgtc
     9301 ctactaaatt acgtgatcct attttaatat gaggatgtat tgttggcaca acatgactta
     9361 cgttccctgt atccgtagag ccataaccaa aatcatcatc aataactgct tcaccaactt
     9421 cttcagcata tttagcaaat aaatcatcta atttcggcgt tttaatgaat tcattcacac
     9481 cgttttgaat tggaccaaat tcataatcac aaccagtctg tatcgcagct ccacgtgcga
     9541 tttgatttac tttttctgtt aatatatcca attctttacg cgtcattgct ctagtataaa
     9601 aacgagcatg tgtatagtct ggaataatat tagctgcttt cccgccatct aaaatcacac
     9661 catgcacacg ttgatctttt ttaatatgtt gtcgtagttg tgctacacca ttaaaataac
     9721 taatcatagc gtctaatgca tttaacgctt catctgcatt ttcagaggca tgagcacttt
     9781 ttccgtaaaa tttaacatct aaaacatcca ctgccaaagt atcaatcgtt ttataagttt
     9841 catttcccgg atgaatcatt aaggcaatgt ctatttgatc aatcacacca gccttgacat
     9901 aagaagcttt agcgctacca ttttccccac cttcttcagc tggacatcca agaacgacta
     9961 ctttaccacc aatttggtca atcacttgct tcaaaccaat tgcaccaagc acacttgcag
    10021 ttccaatgat attatgacca caagcatgac ccaaccctgg caaagcatcg tattctgcta
    10081 aaaaacctat agctggcccg tcaagacctg aatcatatgt cgctataaac ccagttgcat
    10141 gcccagcaat ctcggtttca atttcaaaat catgctcttt caaacgatct attaaagttc
    10201 gagacgcaaa tatttcttca ttaccaagtt caggacgttc atgaattcta tgactgattt
    10261 cgatataact atatttattt gtttctatat aatcgagaat ttgttgtttt tcactcattt
    10321 tttactatct cccttttacc ctacactcat tttaatcatc cacaatattt tgttctttca
    10381 aaatgaatta ttacttattc tatcggtttt atctcatgat gtcatctagt ttttctttat
    10441 ttacaaaatt ttctaataac taaaagcctt tcataaattt ataaaacagt ttcaaattgt
    10501 aaaacataag ccctattgtt acaatttaag tattgttaga aacacaactt acaacaaact
    10561 taagttttat attgcaacaa atcataagcg ttttatagtt ttgagtagta aataggagga
    10621 aattcaaaat gacaaaaatg aatgttgaaa gtttcaattt agatcatact aaagtggttg
    10681 ccccatttat tagattagcg ggaacgatgg aaggattaaa cggagatgtc attcacaaat
    10741 acgacattcg tttcaaacaa ccaaacaaag aacatatgga tatgccagga ctacattcat
    10801 tagaacattt aatggctgaa aatattagaa atcatagtga caaagttgtt gatttaagtc
    10861 ctatgggttg ccaaactggt ttctatgtat catttattaa tcatgataat tatgatgatg
    10921 tattaaatat tgttgaagca actttaaatg atgtgctaaa tgctactgaa gtgcctgctt
    10981 gtaatgaagt acaatgtggc tgggcagcaa gtcattcatt agaaggtgct aaaactatcg
    11041 ctcaagcatt tctagacaaa cgaaacgaat ggcatgatgt tttcggtaca ggaaaataaa
    11101 tcttagtcaa tcaagttaat cagaaaagca gtcgaacaat gatgttacaa tcgccattgt
    11161 ccaactgctt tttattatgc ttcaaagtca aaaaatcgaa caaatgaaaa agtaaaatct
    11221 ttaacatttg tccgatttat ttgagaacca ctataatttc ttaattagtc ccattaacac
    11281 gaactgcata ggtaacctta aatatagttg ccatgttggc aattgtttat ctcctaaagg
    11341 taacttttta actgccatat agatattagc tgggaataca gctagcaaga atagattgat
    11401 tgtatttttc aagcattgag atggtctttt aattaaaagt gcaagtccaa ataatatctc
    11461 aaagattcct gtaacaagaa ccgctgtttt tcgaagtggc aaacatttcg gtatgatatt
    11521 tctaaattgt cgttctcgtg taaaatgcaa tacacctatt acactaaaac ctattcctaa
    11581 taaatatctt agtatgttca atcagcttca actcctactc tgtaatgatt ttatgaatta
    11641 atgtaggcga tacaacatga tcagcaattg ttatgcttga atctaatttt ttaacaacat
    11701 cgtctacatc ttggcgatta ctgtgaatcg ttaataatga ttctccttct tctactttat
    11761 caccaatttt tttatttaaa acaataccaa ccgctaaatc aatatcatcc tcttttgtta
    11821 aacgtcccgc tcctaacatc atcgaagcga cacctatatc gttagagact aattcagtca
    11881 cataacctga ttttttagct ttatattcaa tttgatattg agcttgtggc aaacgctctg
    11941 gatggtcaat aacagtttcg tcgccacctt ggtttttaat aaatgttttg aatttttcta
    12001 atgctgcacc tgaattaatt gcctcaatta gcaacgctct cgcttcttca agcgtttcag
    12061 ctttgtttgc aagtacaacc atttgagaac ctaatgttaa tacaagttct gttaaatctt
    12121 tcggaccttg tcctttcaac gtatcaattg cttcttgtaa ctcaagcgca ttgccaatcg
    12181 cacgtccaag tggctgattc atatcagaaa taatcgccat cgtattacgt cccacattat
    12241 taccaatacg taccattgcg tgcgctaatg cttcagcatc ttctaatgtt ttcataaatg
    12301 caccgctacc agtttttaca tctaatacaa ttgcatctgc accagcagca atctttttac
    12361 tcataattga agaggcaatt aatggtattg aattgacagt accagtaaca tcccttaagg
    12421 catataattt tttgtctgca ggagttaaat ttcctgattg tcctacaact gccactttat
    12481 tttcattaac caatttcaca aatgttgctt catctatttc aacatgaaaa ccatcaattg
    12541 cttctaattt atcaatcgta ccacctgtat gacctaatcc acgcccactc atttttgcaa
    12601 caggaacatc tacagctgct actaatggtg ctaaaaccaa tgtagttgta tctcctacac
    12661 cacctgttga gtgcttatct actttgacac ctttaatatc actcaaatct atcatatcac
    12721 cagaattaac catagccatc gttaatgctg cacgctcatc atcattcata tcttggaaat
    12781 aaatcgccat tgctaaactt gatgcttggt aatcaggaat atccccttta acatagccgt
    12841 caataaagaa attaatttct tccgttgtta gtgtatgacc gtcacgcttt ttctcaataa
    12901 tgtctatcat tctcattttt atcatccttt tcttaaaaag cttaggacaa agcatctgcg
    12961 ctttctctag tccattttta aaagcacaag cgaaaattat tatagcaagc tatcgatttc
    13021 atttttaata tcacaatttc aatgcgatgt tattattctt aaatagattg gttataacgt
    13081 taaagtccct attaaattat cttagaatca tcatggcatt tatgatgtct taaagctgat
    13141 atcgacatac ttatatatgg ttacgatgtc ccatgcttac atatttttat aaaattagta
    13201 atctgaatct gcttctaaac cttgcataat ttgaacgcct gcgctcgcac caatacgtgt
    13261 cgcacctgct tcaaccattt tattgaaatc ttctaaatta cgtacgccac ctgatgcttt
    13321 tacttctaca tcagcaccta ctgtatcttt cattaattta acgtcttctg cagtcgcacc
    13381 gccacctgca aaacctgttg aagttttaac gaagtccgca ccagccactt ttgttaattc
    13441 actcgctttt acgatttcgt catggtccaa caataccgtc tcaataatca cttttactgt
    13501 gtgacctttc gcagctttca ccactgcttc aatgtcttgt tgtacatcat caaaacgtcc
    13561 atcttttaat gcgccgatgt tgatgaccat gtcaatttca tctgcaccat tttgaatcgc
    13621 atcttctgtt tcaaatgctt tcgtcgcagt tgtcgatgca cctaatggga atcctattac
    13681 cgtacaaact aacacctctg aatcagctag tcgctctgct gcatatttaa catgcgttgg
    13741 attcacacat acagatttaa aatggtatgc tttcgcttca tcgatgattt gatcgatttg
    13801 cgtacgtgtt gactcaggct tcaataaagt gtgatcaatc aattttgcac tattcatttc
    13861 tatctcctcc tttatggttg attataaaaa tacggttgta aattagttga ttgagcgtca
    13921 ggttcattta aatatcaggt tagatgttcg ctttttatgt aaccgcatac atatactatt
    13981 acattaattc atttcccata aacaaacaat acaattgaac gtgatatctt cattatgaac
    14041 gatgacttga caacaagcta atcaggatta tatttttata attctttaat tctatagtac
    14101 aaaaattcgc aaaaaaggga aacaaatgtt atcttaaaat tattaatgaa tattaaggag
    14161 aagataacaa atgacaaaag gtacaccaca tattcaacca aatggagtaa aaattgctaa
    14221 aacagtatta atgcctggcg atccgctacg cgcaaaatat attgctgata attttttaga
    14281 aaatgttgaa caatttaacg atgtacgtaa catgtttggt tacactggta catataaagg
    14341 taaagaagtt tctgtaatgg gttctggtat gggtattcca agtattggta tttactcata
    14401 tgagttatac aacttctttg atgtagatac aatcattcgt atcggttctt gtggcgcatt
    14461 acaagaaaat gttaacttat acgatgttat tattgcacaa gctgcatcaa ctaattcaaa
    14521 ttatgtagat caatacaata ttccaggtca tttcgcgcct atcgctgact tcgagttagt
    14581 aactaaagct aaaaatgtcg ctgaccaaat cggtgctact acacacgtag gtaacgtatt
    14641 atcttctgat acattttaca atgccgatcc aacattcaat gatgcttgga aaaaaatggg
    14701 tattttaggt atcgaaatgg aatcagctgg tttatattta aatgcgattc atgctggtaa
    14761 aaaagcactt ggtattttca cagtaagtga tcatatttta cgtgacgaag ctactacacc
    14821 tgaagaacgt caaaattcat ttacacaaat gatggaaatc gctttagaaa tcgcagagta
    14881 acttatttaa attgacttta attgctcttt aacaatgcga ttaaactcaa aaagccaaca
    14941 cattctgggc gtatccccat ttatgtgttg gcttttattt atattattac ttatctgtag
    15001 attagcttaa gtaagattta aacatccaat tatgtttatc tactgatgtt tgcataccta
    15061 taaacatatc ttctgataca tcatcgccag cattaccagc aatttcgatt gcgttttcta
    15121 attgttttga gatatttgtg aagtcttgtg ataattcttc aaccatttgt tctgcagagt
    15181 aacctttcgc agcttcttta acaattgatt gctctaagca ttcagttaat gtacctacag
    15241 ggtttcctcc taccgctaaa attctttcag ctaattcgtc tacatattgg cttgcttcat
    15301 tatataattc ttcaaattta acgtgtaatg agaagaagtt aggtcctttc acataccagt
    15361 ggaaattatg tagctttgtg taagctactg tccagtttgc tacttgttga ttcaattctt
    15421 ttacaacatc ttgttgatta ctcatattta atacactcct taaaattgtc tacgtcttgc
    15481 ttgtttatat taattattat attataatga ttctaatctg taaagtgttt ttactctctt
    15541 ttttataatt attctaatct aaatgtaagg tgttccgctt tttaaatgtc taaattttaa
    15601 ttcatcatct agtatatcca ttatcaatta gcaagtatgt tttatcaatc agtaagtttt
    15661 actttcgcat tatcatctaa aaagaacgat ttttcataat catttggttg gtgccatgtt
    15721 gttttccaca ttctatttct attattagca aacattcgat aaccgaaatt acggactggt
    15781 ttaggtacaa gccataaacc tacgcccaat aatttagttc gattaggtag tgctgtgatt
    15841 agtttaataa tcgcctgcga ttcaaaatat atacgatcac ctttctgtaa aatcacacta
    15901 tttttatttg ccgcttctgg atgttgttta aaaaattgtt gtccaacttc accttttaac
    15961 gtggcaaatt gataatttct tggtaggtca tgttgaatta accaaatgac atagttataa
    16021 caatatatac agtttccatc ataatatacg attggcataa tttattcgct cctttaatag
    16081 tacttatagt catttttcct aatttaaaaa catttaaaca aggttgtcac ttgataagtc
    16141 ctcgcgacac gaaattgcca taaaatttat ttttcagttt tatatt
//
LOCUS       DANBWH010000040        15780 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.042, whole genome shotgun sequence.
ACCESSION   DANBWH010000040 DANBWH010000000
VERSION     DANBWH010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15780
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.042"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            26..796
                     /locus_tag="PPY92_000908"
     CDS             26..796
                     /locus_tag="PPY92_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDJ2624927.1"
                     /translation="MMKRLNKLVLGIIFLFLVISITAGCGIGKEAEVKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIINSEMVIQPNNEDMVAKGMVLYMNRNTKTT
                     NGYYYVDVTKDEDEGKPHDNEKRYPVKMVDNKIIPTKEIKDKNIKKEIENFKFFVQYG
                     NFKDLSKYKDGDISYNPEVPSYSAKYQVTNDDYNVKQLRKRYDIPTNKAPKLLLKGTG
                     NLKGSSVGYKDIEFTFVEKKGENIYFSDSLHLEPSEDK"
     gene            848..1618
                     /locus_tag="PPY92_000909"
     CDS             848..1618
                     /locus_tag="PPY92_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDJ2624928.1"
                     /translation="MIKRVNKLVLGISFLFLIISIFAGCGTGKEAEIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIVRSSMSIQSNGKDMNIKGMVLYMNRNTRTT
                     NGYYYVDVIEREDKGIHRDNEKRYPVKMVDNKIIPTKEIKDEKIKKEIENFKFFVQYG
                     DFKDLSKYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYNIPTNKAPKLLLKGTG
                     NLKGSSIGYKKIEFTFVEKKGENIYFSDGLHFNPSEDK"
     gene            1683..2453
                     /locus_tag="PPY92_000910"
     CDS             1683..2453
                     /locus_tag="PPY92_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDJ2624929.1"
                     /translation="MMKRLNTLVLYISFLILIISIVAGCGTGKEAEIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDEFDKNDKGTWTISSEMAIQKKGEALNIKGMVLKLNRNTRSA
                     KGFYYVNAIKKDENGRPQDKQIEYPVKMIDNKIIPTKDIKDEKIKKEIENFKFFAQYG
                     NFKDLTKYKGGDISYNPEAPIYSAKYQLTNDDYNVKQLRKRYDIPTNKAPKLLLKGSG
                     NLDGSSIGYKKIEFTFVEKKGENTYFTANLHFKPSNDE"
     gene            2518..3288
                     /locus_tag="PPY92_000911"
     CDS             2518..3288
                     /locus_tag="PPY92_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDJ2624930.1"
                     /translation="MMKRLNKLVLGISFLFLVISITAGCGMGKEAEIKKSFEKTMSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIVNSQMAIQNKGEPMKSKGMVLYMNRNTRTT
                     NGYYYVNVIKGEDKGKHQDNEKRYPVKMVDNKIILTKEIKDENIKIEIENFKFFVQYG
                     NFKDLENYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYDIPTNKAPKLLLKGTG
                     NLKGSSVGYKDIEFTFVEKKEENIYFSDGLIFKPSEDK"
     gene            3355..4122
                     /locus_tag="PPY92_000912"
     CDS             3355..4122
                     /locus_tag="PPY92_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDJ2624931.1"
                     /translation="MKRLNKLVLYISFLILVISFTAGCGMGKEAEIKKSFEKTLSMYP
                     IKNLEDLYDKEGYRDDQFDKNDKGTWIINSEMVVQPKGERMKSKGMVLYMNRNTKTTT
                     GKYIVSETLHDEDGRPKSKDKEYPVKMVDNKIIPTKGIKDENIKKEIENFKFFAQYGS
                     FKDLSKYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYKIPTNKAPKLLLKGSGD
                     LKGSSVGYKDIEFTFVEKKGENTFFTDSLHLEPSEDK"
     gene            4219..6456
                     /locus_tag="PPY92_000913"
     CDS             4219..6456
                     /locus_tag="PPY92_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDJ2624932.1"
                     /translation="MQRDYLIRVETESMPDFKRLNGLMIGFVIKGEAHIYDENNMTQC
                     NSGDIFIINHRDLYRFQLQQDGIICYIQFQMKYLADKFDDAHCLYFHLTDATTTKNIH
                     QLRNIMARLVSTHIRHNELSKLTEQQLVIQLLMHMIHYVPRTYHSNQSILNDDKVNQV
                     CDYIELHFHEDLSLSELSEYVGWSESHLSKKFTESLGVGFQHFLNTTRIEHAKLDLTY
                     TDETITDIALQNGFSSAASFARTFKHFTHQTPKQYRGDRPAITENQQSAQHNYHDREL
                     ILLLNDYIEEMNHFIEDIEKMNYKEIAFKPTNQQLNQFNHIIQVGYLRNLLNTQYQSQ
                     LLTCHHDFQVNEVLAYDVMPYIMKKLNAPFTYDAEISNIFYDIDLCLDFLLDHNFSLT
                     MHLNQYDSRDYIDAFKVFIHHVALHVSHRKDLKFNLYVTTLHTSLIEMIDYFKALFPN
                     GGLYIHLDQATERHLPLLKRLEPHINHFVFDANSNDAVDFNKMNDDEFKTASQMIINK
                     TNYLIDLMHRHNLKRPLILLNRNTLTGDTFITNGEYFRGGIIIEQLLKLSTKVEGIGY
                     WLNYDLHVSHCRNERDYMNSIELFHQYNGKRPVYFTALLFNKLTSNILYSDDTCIVTG
                     TDSNFQILLYDAKHFNPYLALDNQMNMRATEMIHLNINALEDGMYKIKHFTLDKENGA
                     LFNLWRKHHTIHGMDKDSIDYVNRMSFPKLEVYDIDITDTLALNIKMITNGIHLIEVK
                     RYPSS"
     gene            6607..7785
                     /locus_tag="PPY92_000914"
     CDS             6607..7785
                     /locus_tag="PPY92_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDJ2624933.1"
                     /translation="MNQQLIEALKSKEDKMIEIRRYLHQHPELSFHEDETAKYIAEFY
                     KGKDVEVETNVGPRGIKVTIDSGKPGKTLAIRADFDALPITEDTGLSFASQNKGVMHA
                     CGHDAHTAYMLVLAETLAEMKDSFTGKVVVIHQPAEEVPPGGAKAMIENGVLDGVDHV
                     LGVHVMSTMKTGNVYYRPGYVQTGRAFFKLKVQGKGGHGSSPHMANDAIVAGSYFVTA
                     LQTVVSRRLSPFETGVVTIGSFDGKGQFNVIKDVVEIEGDVRGLTDATKATIEKEIKR
                     LSKGLEDMYGVTCTLEYNDDYPALYNDPEFTEYVAKTLKEANLDFGVEMCEPQPPSED
                     FAYYAKERPSAFIYTGAAVEDGEIYPHHHPKFNISEKSLLISAEAVGTVVLDYLKGDN
                     "
     gene            7787..9175
                     /locus_tag="PPY92_000915"
     CDS             7787..9175
                     /locus_tag="PPY92_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDJ2624934.1"
                     /translation="MNETYRGGNKLILGIVLGVITFWLFAQSLVNVVPNLQQSFGADM
                     GTISIAVSLTALFSGMFVVGAGGLADKIGRVKMTNIGLLLSIIGSALIIITNLPALLI
                     LGRVIQGVSAACIMPSTLAIMKTYYQGAERQRALSYWSIGSWGGSGICSLFGGAVATT
                     MGWRWIFIFSIIVAVLSMLLIKGTPETKSEITNTHKFDVAGLIVLVVMLLSLNVVITK
                     GAALGYTSLWFFGLIAIVIVAFFIFLNVEKKVDNPLIDFKLFENKPYTGATISNFLLN
                     GVAGTLIVANTFVQQGLGYTALQAGYLLITYLIMVLLMIRVGEKLLQKMGSKRPMLLG
                     TFIVVIGIALISLVFLPGIFYVISCVVGYLCFGLGLGIYATPSTDTAISNAPLDKVGV
                     ASGIYKMASSLGGAFGVAISGAVYVGAVTATSIHTGAMIALWVNVLMGIMAFIAILFA
                     IPNDDKRVKDAK"
     gene            complement(9659..11320)
                     /locus_tag="PPY92_000916"
     CDS             complement(9659..11320)
                     /locus_tag="PPY92_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDJ2624935.1"
                     /translation="MSVTEVIFSFLGGLGIFLYGLKIMGDGLQASAGDRLRDILNKFT
                     SNPVLGVIAGIVVTILIQSSSGTTVITIGLVTAGFMTLKQAIGVIMGANIGTTVTAFI
                     IGIDLGEYAMPILALGAFLIFFFKRSKINNIGRILFGFGSLFFGLEFMGDAVKPLASL
                     DGFKQLMLDMSSNPILAVIVGAGLTALVQSSSATIGILQEFYQQDLISLNAAIPVLLG
                     DNIGTTITAILASLAGSIAAKRAALVHVIFNLIGVIIFTIFLPVVIHLISLLQDVWHL
                     KPAMTIAVSHGIFNITNTLIQLPFVAGLAWIVTKLVPGKDIADDYKPQHLNKDLVYHA
                     PGVALQETQKELQNVGQIVLSMFEDIREITKDDKKLIKKLEQKHQAVETINDSIRNYL
                     VRISTKAITKADVERLAVMFDVNRSILKVAELTEEYVAQLKRQHDEDIRITEDAQRGM
                     DKLFNHVAESFDKAIDMLDVYDKTKKDEIVERSRESFNIEHKLRKGHIKRLNRGECTT
                     KGGLLYIDMIGVLERIGYHSRNVSEALVGLNDDVPTDEEIATTEI"
     gene            complement(11645..13420)
                     /locus_tag="PPY92_000917"
     CDS             complement(11645..13420)
                     /locus_tag="PPY92_000917"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HDJ2624936.1"
                     /translation="MYYSYGNYEAFARPKKPENVENKSAYLIGSGLASLAAACFLIRD
                     GQMEGSKIHILEELPKAGGSLDGENMPLKGYVVRGGREMENHFECLWDLFRSIPSLEI
                     DNASVLDEFYWLNKEDPNYSRCRVIEKQGQRLVTDGDFTLTKTAIKEILDLCLTNEED
                     LDDVKITDVFSDDFFNSNFWIYWKTMFAFEPWHSAMEMRRYLMRFVHHIGGLADFSAL
                     KFTKYNQYESLVLPMVEYLKSHGVQFEYDVKVEDIKVDVTTSQKIAREILIHRHGKAE
                     SIKLTVDDLVFVTNGSITESSTYGDNDTPAPPTDELGGSWTLWKNLARQSPEFGNPDK
                     FCQNIPKKSWFVSATSTTNNKEIIDTIESICKRDPLAGKTVTGGIITINDSAWQMSFT
                     INRQQQFKDQPENEISTWIYALYSDVNGDYIKKPITECSGNEICQEWLYHLGVSTDKI
                     EDLAKHVSNTIPVYMPYITSYFMTRAIGDRPLVVPHQSQNLSFIGNFAETERDTVFTT
                     EYSVRTAMEAVYQLLNIDRGIPEVINSTFDLRVLMDAVYELNDHQDLREITKDSKMQK
                     LALAGFLKKIKGTYIETLLKEHKLL"
     gene            complement(13594..14466)
                     /locus_tag="PPY92_000918"
     CDS             complement(13594..14466)
                     /locus_tag="PPY92_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDJ2624937.1"
                     /translation="MNKLRDTTFLSYLFTIILWGSAFPMIKIALNDFSAESLSAFRLI
                     LATIILLPFVIIKKLPTPELRDIPVIFILGFCGFVIYHTALNFGETLISAGISGILVS
                     TTPIFSSALAYIFLKEHFSKWNWLSSLVAFIGISIISISKDDYTTINVLGVFIILLAS
                     FSESLYFTFQKKYIEKYGFIAFTLYTIMASSPFMLIFIPEIINDIHGATFTSIVSVLY
                     LAIFPTIIPYVLLAYIVKSVGVSDATMSLYLTPIVSLLLSYLLLDELPTTLAIIGGII
                     TLLGVSLSNFFQNT"
     gene            14579..15780
                     /locus_tag="PPY92_000919"
                     /pseudo
     CDS             14579..15780
                     /locus_tag="PPY92_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498667.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
BASE COUNT         5672 a         2309 c         2865 g         4934 t
ORIGIN      
        1 attttaaaaa gaagagagtt gtaagatgat gaaacgatta aacaaattag tgttaggcat
       61 tatttttctg tttttagtca ttagtatcac tgctggttgt ggcataggta aagaagcgga
      121 agttaagaaa agctttgaaa aaacattgag tatgtaccct attaaaaatc tagaggattt
      181 atacgataag gaaggctatc gtgatgatca gtttgataaa aatgataaag gtacatggat
      241 tataaattct gaaatggtta ttcaacctaa taatgaagat atggtagcta aaggcatggt
      301 tctatatatg aatagaaata ccaaaacaac aaatggttac tactatgtcg atgtgactaa
      361 ggacgaggat gaaggaaaac cgcacgacaa tgaaaaaaga tatccggtta aaatggtcga
      421 taataaaatc attccaacaa aagaaattaa agataaaaac ataaaaaaag aaatcgaaaa
      481 ctttaagttc tttgttcaat atggaaactt taaagatttg tcgaagtaca aagatggaga
      541 tatttcatac aatccagagg tgccaagtta ttcagcaaaa tatcaagtaa ctaatgatga
      601 ttataatgta aaacaattac gtaaaagata tgatataccg acgaataaag caccaaagtt
      661 attgttgaaa ggtacaggta atttaaaagg ctcatcagtt ggatataaag atattgaatt
      721 tactttcgtg gagaaaaaag gtgaaaatat atactttagt gatagtctac atcttgaacc
      781 aagtgaggat aaataaacgt gaccaataaa gagtatgaaa tcgaacccgg aaaaagagag
      841 tgatgaaatg ataaaacgtg taaataaatt agtgctaggt attagttttc tgtttttaat
      901 catcagtatc tttgctggtt gtggtacagg gaaagaagcg gaaataaaga aaagttttga
      961 aaaaacgttg agtatgtatc cgattaaaaa tctagaggat ttatacgata aagaaggata
     1021 tcgtgatgat caatttgata aaaatgataa gggaacatgg attgttcgtt cgtcaatgtc
     1081 gattcaatct aatggaaaag atatgaatat aaaaggcatg gttttatata tgaatagaaa
     1141 tacaagaaca acaaatggct attactatgt tgatgttata gaaagagaag ataaaggtat
     1201 acatcgtgat aacgaaaaaa gatatccggt taaaatggtc gataataaaa tcattccaac
     1261 taaagaaatt aaagatgaaa aaataaaaaa agaaatcgaa aactttaagt tctttgttca
     1321 atatggcgac tttaaagatt tgtcgaagta caaagacggc gatatatcat acaatccaga
     1381 ggtgccgagt tattcagcaa aatatcaatt aactaatgat gactataatg taaaacaatt
     1441 acgaaaaaga tataatatac caacgaataa agcaccaaag ctattgttga aaggtacagg
     1501 gaatttaaaa ggttcatcaa ttggctacaa aaaaattgaa tttactttcg tagagaaaaa
     1561 gggagaaaat atatacttta gtgatgggct acattttaat ccaagtgagg ataaataatc
     1621 atgattcgat caaattatga aatcgatcca ggaaaaacaa taaaaagaaa taggattgtg
     1681 aaattatgaa acgattaaat acattggtat tgtacatcag ttttttgata ttaatcatca
     1741 gtatcgttgc tggttgtggt acagggaaag aagcggaaat aaagaaaagt tttgaaaaaa
     1801 cattgagtat gtatccaatt aaaaatctag aggatttata cgataaagaa ggctatcgtg
     1861 atgacgaatt tgataaaaat gataaaggaa cgtggactat tagttctgaa atggcaattc
     1921 agaaaaaggg agaagcctta aatataaaag gtatggtttt gaagttaaat agaaatacaa
     1981 ggagtgcaaa aggattttac tatgttaatg cgataaagaa ggatgaaaat ggcagacctc
     2041 aggataagca aatagaatat cccgttaaaa tgattgacaa taaaatcatt ccaactaaag
     2101 atatcaaaga tgaaaaaata aaaaaagaaa tcgaaaactt taagtttttc gcgcagtatg
     2161 gcaattttaa agatttgacg aagtataaag gtggagatat ttcatataat ccagaagccc
     2221 cgatttattc agcaaaatat caactaacca atgatgatta taatgtaaag caattacgta
     2281 aaagatatga tataccgacg aataaagctc caaagttatt gttaaaaggt tcaggaaatt
     2341 tagatggttc atcaattgga tacaaaaaaa ttgaatttac tttcgtagag aaaaaaggag
     2401 aaaacactta ctttacagct aacctacatt ttaaaccgag taatgatgaa taatcgtgac
     2461 taataaagag tatgaaatcg attcaaggaa acaaaataaa aagaagagag ttgtaaggtg
     2521 atgaaacgtt taaataaatt agtgttaggt attagtttct tatttttagt cattagtatt
     2581 actgctggtt gtggcatggg taaagaagcg gaaattaaga aaagttttga aaagacaatg
     2641 agtatgtatc cgattaaaaa cctagaggat ttatatgaca aagagggcta tcgagatgat
     2701 caatttgata aaaatgataa gggaacatgg attgtaaatt ctcaaatggc aattcaaaat
     2761 aaaggagaac ctatgaaatc aaaaggcatg gttctgtata tgaatagaaa tacaagaaca
     2821 acaaatggct attattatgt taatgtgatt aagggcgagg ataaaggtaa acatcaagac
     2881 aatgaaaaaa gataccctgt taaaatggtc gacaataaaa taatccttac taaagaaatt
     2941 aaagatgaaa acataaaaat agaaatcgaa aactttaagt tttttgttca atatggaaac
     3001 tttaaagatt tggaaaatta taaagatgga gatatatcat ataatccaga ggtgccaagt
     3061 tattcagcta aatatcaatt aactaatgat gattacaatg taaagcaatt acgtaaaaga
     3121 tatgacatac caacaaataa agcaccgaag ttgttgttga agggtacagg gaatttaaaa
     3181 ggttcatcag ttggatataa agacattgaa tttacgtttg tagagaaaaa agaagagaac
     3241 atttacttta gtgatgggtt aatttttaaa ccaagtgagg ataaataatc atgactcgat
     3301 taaaatatga aatcaatcca ggaaagataa taataagaat aggattgtga aattatgaaa
     3361 cgattaaaca aattggtatt gtatattagt tttttgatat tagtcattag tttcactgct
     3421 ggttgtggca tgggtaaaga agcggaaata aagaaaagtt ttgaaaaaac attgagtatg
     3481 tatccgatta aaaatctaga ggatttatac gataaagaag gctatcgtga tgatcaattt
     3541 gataaaaacg ataaaggcac atggattatt aattctgaaa tggttgttca acctaaaggg
     3601 gaacgtatga aatcaaaagg tatggttcta tatatgaata gaaacactaa gactacaacc
     3661 gggaagtata tagtgagtga aacacttcat gatgaagatg gtagacctaa gagtaaagat
     3721 aaagaatatc cggttaaaat ggtagacaat aaaatcattc caactaaagg tatcaaagac
     3781 gaaaatataa aaaaagaaat cgagaatttt aagttcttcg cgcaatatgg cagctttaaa
     3841 gatttgtcga agtacaaaga cggcgatata tcatataatc cagaggtacc gagttattca
     3901 gcaaaatatc aattaactaa tgatgactat aatgtaaaac aattacgaaa aagatataaa
     3961 ataccaacga ataaagcacc aaagttattg ttgaaaggtt cgggagactt aaaaggttca
     4021 tcagttggat ataaagacat tgaatttact tttgtggaga aaaaaggtga aaatactttt
     4081 tttactgata gtctacatct tgaaccaagt gaggataaat aatctcctgc ctcccacacg
     4141 attcaacacg aatttcaaat atgttgctgt aaaataatgt aaaataaacg tattcatatt
     4201 actgaattga gggatagtat gcaacgtgat tatttaattc gagtagaaac tgaaagcatg
     4261 ccagatttca aaaggctcaa tggtttaatg attggttttg ttattaaagg tgaggcacat
     4321 atttatgatg aaaataacat gacgcaatgc aacagtggcg acattttcat cattaaccac
     4381 cgtgacttgt atcgatttca acttcaacaa gatggcatca tatgttatat ccaattccaa
     4441 atgaaatatt tggcagacaa gtttgatgat gcgcattgtc tatattttca cttaacagat
     4501 gcgaccacaa ccaagaatat acatcaactg agaaatataa tggcaagact ggtttcaaca
     4561 cacattcgac ataatgagtt gtctaaattg actgagcaac aacttgtgat tcagttgctt
     4621 atgcatatga ttcattatgt cccgcgtaca tatcattcga accaaagtat cttaaatgat
     4681 gataaagtga atcaagtatg cgactatatc gagttacatt ttcatgaaga tttaagcctt
     4741 tcagaattaa gcgaatacgt tgggtggtca gagagccatc tgtctaaaaa gtttacagaa
     4801 tcgctaggtg taggattcca acatttctta aatacgacgc gaattgagca tgcgaaactc
     4861 gatttaacat acacagatga aacgattact gatattgcat tgcaaaatgg cttttcaagt
     4921 gcagcgagct ttgcgagaac atttaaacac tttacgcatc aaacgcctaa acaatatcga
     4981 ggtgatcgtc cagcaatcac tgaaaatcaa caatcggcac aacataatta tcacgaccgt
     5041 gaattgatat tacttttaaa tgactacatt gaagaaatga atcatttcat tgaagatatt
     5101 gaaaagatga actataaaga gattgccttt aaaccaacta atcaacaact aaatcaattt
     5161 aatcatatta ttcaagtggg ctatttgagg aatttgctca atacacagta tcaatcacag
     5221 ttgcttacat gtcatcatga ttttcaagtc aatgaagtat tagcatatga tgtgatgcca
     5281 tatattatga aaaagctcaa tgcgccattc acgtatgatg cagagatttc gaatatattt
     5341 tatgatatcg atttgtgttt agacttttta ttagatcata actttagtct aaccatgcat
     5401 ttgaatcagt atgactcacg agattatatc gatgcattca aagtatttat ccatcacgtt
     5461 gccctgcatg tcagtcatag aaaagatttg aagttcaact tgtatgtgac gacattgcac
     5521 acgtctttaa ttgaaatgat tgattatttt aaagcattat tccctaatgg tggcttgtac
     5581 attcacttag atcaagctac ggaaagacat ctaccattgt tgaaacgact tgagccacac
     5641 atcaaccatt ttgtatttga tgccaattca aatgatgctg ttgattttaa taaaatgaat
     5701 gatgatgaat ttaaaaccgc gagtcaaatg attattaata aaacgaatta ccttatcgac
     5761 ttaatgcatc gccataacct aaagcgtcca ctcattttac tcaatcggaa tacattgacg
     5821 ggtgatacat ttattacaaa cggcgaatat tttagaggtg gtatcatcat tgagcaatta
     5881 ttaaaattaa gtactaaagt agagggtatc gggtattggt tgaattatga tttgcacgtc
     5941 agtcattgta gaaatgaacg ggattatatg aattctattg aactgtttca tcaatataat
     6001 ggaaaacgtc cggtctattt cacggcattg ctatttaata aattaacaag caatattttg
     6061 tattctgatg atacatgtat tgtcacggga actgattcaa attttcaaat attgttatat
     6121 gatgcaaagc attttaatcc gtacttagcg ttggacaatc aaatgaatat gcgtgcaacg
     6181 gaaatgatcc atttgaacat taatgcccta gaagacggta tgtataagat taaacatttt
     6241 accttagata aagaaaatgg tgcgttattt aatctttggc gcaaacatca tacgatacat
     6301 ggcatggaca aggactctat agattacgtt aatcgaatga gttttccgaa attagaagta
     6361 tatgatatag atatcacgga cacactggca ttaaacatta aaatgattac gaatgggatt
     6421 cacttaattg aagtaaaacg ttacccaagt tcataaaatg atcacaaatc acaaattttg
     6481 atatacataa tttgtgattt tttatattca aagctaaaat tgcaaaaaat taatggttaa
     6541 catctctgtt gttggcaata taaataatga ttaatcattt atgatgtaac taaggagatg
     6601 aaggatatga atcaacaatt aattgaagct ttaaaatcta aagaagacaa aatgattgag
     6661 atcagacgtt atttacatca gcatccagaa ttatcttttc atgaagatga aacggcgaaa
     6721 tacatcgctg aattttacaa aggtaaagat gtggaagtag aaacgaatgt cggaccacgt
     6781 ggaattaaag taacgattga ttcagggaaa cctggtaaaa cattagcaat ccgtgcagac
     6841 tttgacgcat tacccattac tgaagataca ggattatctt ttgcatcaca aaataaaggt
     6901 gttatgcacg catgtggtca cgatgcacat acagcttaca tgcttgtatt agcagagacg
     6961 cttgctgaaa tgaaagatag ttttacagga aaagtcgttg tgatacatca accagctgaa
     7021 gaagtaccac caggtggtgc taaagcaatg attgaaaatg gtgtattaga cggtgttgat
     7081 catgtattag gtgtacacgt catgagcaca atgaaaacag gtaatgtgta ttacagacct
     7141 ggttatgttc aaacaggacg cgcattcttc aaattgaaag ttcaaggtaa aggtggtcat
     7201 ggttcatcac cacatatggc caatgatgcc attgttgcag gtagctactt cgtcacagcg
     7261 ttacaaacag ttgtatctag acgactaagt ccatttgaaa ccggtgttgt cacaatcggt
     7321 tcatttgacg gtaaaggtca attcaatgtc attaaagatg ttgttgaaat tgaaggtgat
     7381 gtacgtggat taacagatgc tacaaaagca acaattgaaa aagaaattaa acgtttatca
     7441 aaaggattag aggatatgta tggtgtaact tgcaccttag aatataacga tgattatcca
     7501 gcattatata atgatccaga gtttactgag tacgtggcta agacgttgaa agaagcaaac
     7561 cttgattttg gtgttgaaat gtgtgaacca caaccacctt cagaagactt tgcatattat
     7621 gctaaagaac gtccaagtgc ctttatttat acaggtgcag ctgtggaaga tggtgaaatt
     7681 tacccacatc atcatcctaa atttaacatt tcagaaaaat cattacttat ttcggcagaa
     7741 gctgtaggga cagttgtttt agattacctt aaaggagata actaacatga atgaaacgta
     7801 tcgcgggggc aacaagttaa tcttaggtat tgtattaggt gttattacat tttggttgtt
     7861 tgcacaatca cttgtaaatg ttgtaccaaa tttacaacaa agttttggtg cagacatggg
     7921 gacaattagt atagcggtca gtctaactgc actattttca ggtatgtttg tcgttggagc
     7981 aggtggtcta gcagataaaa ttgggcgcgt gaaaatgacg aatatcggtt tattgttaag
     8041 tattattggt tcagcattaa ttattattac gaatttaccg gcattattaa ttttaggtcg
     8101 tgttatacaa ggtgtatcag cagcgtgtat tatgccttcc acattggcca ttatgaaaac
     8161 ttattatcag ggtgctgaac gtcagcgtgc cttaagttat tggtctatcg gttcttgggg
     8221 tggcagtggt atctgttcac tcttcggtgg tgcagttgcg acaactatgg gttggagatg
     8281 gattttcatc ttctcaatta tcgttgccgt actttcaatg ttactcatca aagggacgcc
     8341 tgaaacgaaa tcagaaatta ccaatacaca taaatttgac gttgcagggc taattgttct
     8401 agtagttatg ttgctaagtt taaacgttgt cattactaaa ggtgcagcac ttggttacac
     8461 atcattatgg ttctttggtt tgattgcaat cgtaattgta gcattcttta ttttcttaaa
     8521 tgttgagaaa aaagtagata atccacttat tgattttaaa ttatttgaaa ataaaccata
     8581 tacaggtgca acgatttcga acttcttatt aaacggtgtt gcaggtacat taattgtagc
     8641 gaatacattc gtgcaacaag gtttaggtta tacagcattg caggcaggat acttattaat
     8701 tacttattta atcatggtgt tattgatgat tcgagttggt gaaaaattat tacaaaaaat
     8761 gggttctaag cgaccaatgt tattaggtac attcattgtg gtcattggta ttgcacttat
     8821 ttcattagta ttcttaccag gcatatttta tgttatcagt tgtgtcgtag gatatttatg
     8881 tttcggacta ggcttaggta tttatgcaac accttctaca gatacagcta tttcgaatgc
     8941 accgttagat aaagttggcg ttgcttcagg tatttataaa atggcttcat cacttggtgg
     9001 cgcattcggt gtcgcaatta gtggtgctgt atatgttggt gcagttactg caacgagcat
     9061 tcatacaggt gcgatgattg cactttgggt taacgtatta atgggaatca tggcatttat
     9121 cgcaatttta ttcgcgattc ctaatgatga taaacgtgtc aaagatgcga aataataacg
     9181 atgacacgcc aagtgttttg ataatgatat aaatgaaagt aaagcaaatt aaatgaggaa
     9241 atgtatagag cagtgcatat agataaatct atgattggat taacactaat ataaatataa
     9301 atgaaaaacc atccacttaa acttttgata agttaggtgg atggttattt attgcgttgt
     9361 tctaaaaatt tggacattta tatagaagga actatatata atgtgaaggt tatggcaatt
     9421 cgcttcaatg aaggcgacgt gaaagtgaat actaaatgat tcgatgtcag actccgagaa
     9481 cagtgtccgt catgcttacc gcatactaat gaagcattat tatatcagtg tttatagccg
     9541 atttaagata ataaagtttg ctaaacaaaa aagccaatcc attgatgttg gattgacctt
     9601 gtatatgcct ctgaatctct aattttaaaa aatatgaata taaataagac attaaaaatt
     9661 aaatttcagt tgttgcaatt tcttcatctg taggtacatc atcgttaaga ccaacaagtg
     9721 cttcagaaac atttcgtgaa tgataaccga tacgttcaag aacaccaatc atatcgatat
     9781 atagtaatcc gccttttgtt gtacattcgc cacgattaag gcgtttaata tgacctttgc
     9841 gtagtttatg ttcaatatta aatgattctc tactacgttc tacaatttca tcttttttcg
     9901 ttttgtcata aacatctaac atgtcgatgg ctttatcaaa tgactcagca acatggttga
     9961 ataatttatc cataccgcgt tgtgcatctt ctgtaatgcg aatatcttca tcatgttggc
    10021 gttttaattg agcgacatac tcttctgtta gctctgctac ttttaaaata gagcgattga
    10081 catcaaacat aactgctaaa cgctcaacgt ctgccttcgt aatggctttt gtagaaattc
    10141 taactaaata atttcgaatg ctatcattga ttgtttcaac agcttggtgc ttttgttcaa
    10201 gcttcttgat caatttttta tcgtcttttg taatttcgcg aatgtcttca aacattgata
    10261 agacaatctg acccacattt tgtaattctt tttgagtttc ttgtaatgca acaccaggtg
    10321 cgtgataaac aagatctttg tttaagtgct gaggtttata gtcatcagca atatctttac
    10381 ctgggacaag ctttgtaact atccatgcta aacctgctac aaatggtaat tgaatcaaag
    10441 tatttgttat gttgaagata ccatgtgata ctgcaatcgt catcgctggt tttaagtgcc
    10501 atacatcttg taacaaacta atcaaatgaa tcacaactgg caagaaaatt gtgaagataa
    10561 ttaccccgat taaattaaag atgacgtgta caagcgccgc acgttttgca gcgattgagc
    10621 cggctaaact agctaagata gctgtaattg ttgtaccaat gttatcacct agtaacacag
    10681 ggattgctgc gtttaagctg attaaatctt gttgataaaa ttcttgtaaa ataccaatcg
    10741 tcgcacttga actttgaact agtgctgtta accctgcgcc gacaatgaca gcaagtattg
    10801 gatttgatga catatcaagc attaattgct taaatccatc taatgatgct aaaggtttaa
    10861 cggcatcacc cataaattct agaccgaaga atagagaacc gaaaccgaat agtatgcggc
    10921 caatgttatt gattttagag cgtttaaaga aaaagattaa aaatgcacct aatgctaaaa
    10981 ttggcattgc atattcgcct aaatctatac cgataataaa tgcagttacc gttgttccga
    11041 tattggcacc cattatcact ccaatagctt gttttaacgt cataaatcca gctgttacca
    11101 gtccgattgt gataacggtc gtacctgaac tactttgtat taaaatagtt acaacgatac
    11161 ctgcaataac acctaatact ggatttgatg taaatttgtt taaaatatct cgtagcctgt
    11221 ctcctgctga tgcttgaagc ccgtctccca tgatttttaa gccgtaaagg aaaataccta
    11281 aaccacctaa aaaggagaaa atgacttctg taaccgacat ttccattatt tcacctcaaa
    11341 taagctttat atttagatta tcgcttataa ttgtaaattt aatgttaaga ttaggtaaaa
    11401 ttatttaaca atatatgtta tttgtatatg acttgtaaaa tatcgtcact tattatgtaa
    11461 attttcagtg tgaaatggca ggtttgcaat aaattgttta acaaaatgat gcaatcaatc
    11521 atgtaattat gtttcatcaa aaaaatcatg tgagtgggat aacgaaataa agtttgtgaa
    11581 catatcattt ctatcccact ccatgatttg aaatcaccaa ataaaaatct attaatggtt
    11641 ttcgttataa caatttgtgt tcttttaata atgtctcaat gtacgtacct tttatctttt
    11701 taaggaatcc tgctaatgcg agtttctgca ttttcgaatc tttagtaatc tcgcgcaaat
    11761 cttgatggtc attcagttcg tatacggcat ccattaagac gcgaagatca aatgtactat
    11821 tgatgacctc tggaatacca cgatctatat ttagtaattg ataaacagct tccatggcag
    11881 tacgaaccga atattctgtt gtaaatacag tatctcgctc tgtttctgca aagttaccaa
    11941 taaatgataa attttgtgat tgatgcggga cgactaaagg tctgtcgccg atagcacgcg
    12001 tcatgaaata agaggtgata tatggcatat aaacaggaat cgtattagat acatgttttg
    12061 ctaagtcttc aattttgtca gttgatacac ctaagtgata cagccattct tggcatattt
    12121 cattaccact acattctgta attggctttt taatataatc gccgtttaca tctgaatata
    12181 aggcataaat ccatgtagat atttcatttt caggttggtc tttaaactgt tgctgacgat
    12241 tgattgtaaa actcatttgc catgcagaat cattgattgt aataataccg cctgtaactg
    12301 ttttgcctgc aagtgggtca cgtttacaaa tactttctat tgtatcgata atctctttat
    12361 tgtttgttgt agaagttgct gaaacaaacc aacttttttt aggaatattt tggcaaaact
    12421 tatcaggatt accaaattca ggactttgtc gcgctaaatt tttccatagt gtccaactac
    12481 cacctaattc gtcagttggt ggtgctggtg tatcattatc accatacgta gagctttccg
    12541 taatactacc gtttgtcaca aagacaagat catctacagt cagtttaatt gactctgctt
    12601 tgccatgtcg gtgaattaat atttctcggg caattttttg acttgtcgta acatctactt
    12661 taatatcttc tacttttaca tcgtattcaa attgaacccc atgcgatttt aaatattcaa
    12721 ccataggtaa tactaaagat tcatattgat tatatttagt gaattttaaa gctgaaaagt
    12781 ctgcgagacc accaatatga tgaacgaatc gcattagata acgacgcatt tccatagcag
    12841 aatgccacgg ttcaaatgca aacatcgttt tccagtaaat ccaaaagttt gaattaaaga
    12901 agtcatcgga aaatacatct gttattttga catcatctaa atcttcttca ttcgttaagc
    12961 ataaatctaa aatttcttta atcgccgttt tagtcaaagt gaagtctccg tctgtgacta
    13021 aacgttgacc ctgtttctca ataacacgac agcgagaata gttagggtct tctttgttta
    13081 gccaatagaa ctcatctaat acagacgcgt tatcgatttc taatgaaggg atagatctga
    13141 ataagtccca caaacattca aagtggttct ccatttcacg accaccgcgg acaacatagc
    13201 cttttaaagg catattttca ccatcaagac taccacctgc tttaggtaac tcttctaaaa
    13261 tatgaatctt cgaaccttcc atttgaccat ccctaattaa gaaacaagct gccgcaagtg
    13321 aagctaaccc agacccgatt aagtaagcag atttgttttc tacattttca ggttttttag
    13381 ggcgcgcaaa tgcttcataa tttccataac tgtaatacat atccaccaag tcctttcgta
    13441 ttagaataca ctcagactat acccctttgt gaattgtaaa tattagtatt tatattggta
    13501 aatttgacga aattagatca tttttatatt tttgattttt aaaattttat cactcgaatg
    13561 tttttggatt ctgaggtgcg ggacttggaa taattatgta ttttgaaaga agttacttaa
    13621 actaacgcct agtagagtga taattccgcc tataatagca agggttgttg gtagctcgtc
    13681 taataacaga taagataata ataaagaaac gataggtgtt aaataaagag acattgttgc
    13741 atcagagaca ccaactgact tcacaatata agcaagcaaa acgtatggaa ttatagtagg
    13801 gaatatagct aaataaagta ccgatactat tgatgtaaaa gtggcgccgt gtatatcgtt
    13861 gatgatttca ggaataaaaa taagcataaa tggtgagctt gccattattg tatatagtgt
    13921 gaaagcgatg aagccgtatt tttctatgta ttttttctgg aaagtaaaat acaaactttc
    13981 actaaaagat gcaagtaaaa taataaaaac acctaataca ttaatagttg tgtaatcatc
    14041 tttacttatt gaaataatgg atattcctat aaatgcgacg agtgaactta accaattcca
    14101 ttttgaaaaa tgctctttta aaaatatata agctaaagca ctagaaaaaa taggcgttgt
    14161 agagactaga attccagata tacctgcact aatcaaagtt tcaccaaaat ttaaagctgt
    14221 gtgatatatc acaaatccac aaaatcctaa aataaaaata acagggatat ctcttagttc
    14281 aggggtaggc aatttcttta taattacgaa cggcaagaga attattgttg ctaaaattaa
    14341 acgaaatgcc gacaatgatt ctgcactaaa atcatttaac gcaatcttta tcattggaaa
    14401 tgcagatccc cacaatatga tagtaaataa atatgataga aaagtagtgt ctcgaagttt
    14461 attcattaat atcatcactc ctttaattat gtgtttctat attaaaaaat atgatttaaa
    14521 atgagtacaa ccaattttga atggatttac ctatccaatt ttaaaaggga gggagaagat
    14581 ggctaaatat taagatattg ctagtgacat aagagataaa ataatcacag gggattggtt
    14641 ttatggaatg aagatacctc cacagaggca gttggcgata caatacaacg taaatagagt
    14701 gacgattatt aaaagtattg agttattaga ggctgaagga tttatctata ctaaagtggg
    14761 gagtggaaca tatgttaatg actatttgaa tgaagcacat attacaaata agtggtctga
    14821 aatgatgtta tggtcctctc aacaaagaag tcagtatacg gtgcaattaa ttaataaaat
    14881 tgagacagat gattcgtata tacatataag taaaggtgaa ttgggtatat cgttaatgcc
    14941 acatattcaa ttgaaaaaag ccatgtctaa tacagccagt catattgaag acttatcttt
    15001 tggttataat aatggctatg gttatatcaa gttaagagat attatcgttg aacgaatgtc
    15061 aaagcaaggt ataaatgtag gtagagaaaa tgtaatgatc acttcaggcg ctttacatgc
    15121 cattcaactt ttatctattg ggtttttagg tcaagatgcc ataataattt cgaatacacc
    15181 atcatatatt cactctacaa atgtttttga gcaattgaat tttagacata ttgatgttcc
    15241 ttataatcaa attaatgaaa ttaataccat cattgataga tttattaatt ttaaaaataa
    15301 agcgatttat atagaaccta ggtttaataa cccgacaggt cgttctttaa cgaatgagca
    15361 aaagaaaaat ataattactt atagcgaaag acataatatt cctatcattg aagatgatat
    15421 ctttagagat attttcttta gcgatccaac tcctgctatc aaaacttatg ataaattggg
    15481 aaaagttata catataagca gtttttcaaa aacgattgca ccagcaataa gaataggttg
    15541 gattgttgct tctgaaaaaa taatagagca attggcagat gtaagaatgc aaattgacta
    15601 tggatccagt attttgtcac aaatggttgt atatgagatg ttgaaaaata agtcttatga
    15661 taaacactta gtaaagttaa ggtatgtttt aaaagataaa cgagacttta tgttaaacat
    15721 cctcaataat ttatttaagg atatagcaca ttgggaggtt ccaagtggag gttattttgt
//
LOCUS       DANBWH010000041        15683 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.043, whole genome shotgun sequence.
ACCESSION   DANBWH010000041 DANBWH010000000
VERSION     DANBWH010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15683
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.043"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            68..361
                     /gene="esxA"
                     /locus_tag="PPY92_000920"
     CDS             68..361
                     /gene="esxA"
                     /locus_tag="PPY92_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxA"
                     /protein_id="HDJ2624938.1"
                     /translation="MAMIKMSPEEIRAKSQSYGQGSDQIRQILSDLTRAQGEIAANWE
                     GQAFSRFEEQFQQLSPKVEKFAQLLEEIKQQLNSTADAVQEQDQQLSNNFGLQ"
     gene            444..3473
                     /gene="esaA"
                     /locus_tag="PPY92_000921"
     CDS             444..3473
                     /gene="esaA"
                     /locus_tag="PPY92_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaA"
                     /protein_id="HDJ2624939.1"
                     /translation="MKKKNWIYALIVTLIIIIAIVSMIFFVQTKYGDQSEKGSQSVSN
                     KNNKIHIAIVNEDQPTTYNGKKVELGQAFIKRLANEKNYKFETVTRNVAESGLKNGGY
                     QVMIVIPENFSKLAMQLDAKTPSKISLQYKTAVGQKEEVAKNTEKVVSNVLNDFNKNL
                     VEIYLTSIIDNLHNAQKNVGAIMTREHGVNSKFSNYLLNPINDFPELFTDTLVNSISA
                     NKDITKWFQTYNKSILSANSDTFRVNTDYNVSTLIEKQNSLFDEHNTAMDKMLQDYKS
                     QKDSVELDNYINALKQMDSQIDQQSSMQDTGKEEYKQTVKENLDKLREIIQSQESPFS
                     KGMIEDYRKQLTESLQDELANNKDLQDALNSIKMNNAQFAENLEKQLHDDIVKEPDTD
                     TTFIYNMSKQDFIAAGLNEGEANKYEAIVKEAKRYKNEYNLKKPLAEHINLTDYDNQV
                     AQDTSSLINDGVKVQRTETIKSNDINQLTVATDPHFNFEGDIKINGKKYDIKDQSVQL
                     DTSNKEYKVEVNGVAKLKKDAEKDFLKDKTMHLQLLFGQANRQDEPNDKKATSVVDVT
                     LNHNLDGRLSKDALSQQLSALSRFDAHYKMYTDTKGREDKPFDNKRLIDMMVDQVIND
                     MESFKDDKVAVLHQIDSMEENSDKLIDDILNNKKNTTKNKEDISKLIDQLENVKKTFA
                     EEPQEPKIDKGKNDEFNTMSSNLDKEISRISEKSTQLLSDTQESKTIADSVSGQLNQL
                     DNNVNKLHATGRALGVRANDLNRQMAKNDKDNELFAKEFKKVLQNSKDGDRQNQALKA
                     FMSNPVQKKNLENVLANNGNTEVISPTLFVLLMYLLSMITAYIFYSYERAKGQMNFIK
                     DDYSSKNHLWNNVITSGVIGTTGLVEGLIVGLIAMNKFHVLAGYRAKFILMVILTMMV
                     FVLINTYLLRQVKSIGMFLMIAALGLYFVAMNNLKAAGQGVTNKISPLSYIDNMFFNY
                     LNAEHPIGLALVILTVLVIIGFVLNMFIKHFKKERLI"
     gene            3473..3931
                     /gene="essA"
                     /locus_tag="PPY92_000922"
     CDS             3473..3931
                     /gene="essA"
                     /locus_tag="PPY92_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HDJ2624940.1"
                     /translation="MLMNSVIALTFLTASSNNGGLNIDVQQEEEKRINNDLNQYDTTL
                     FNKDSKEVNDAIAKQKKERQQQIKNDMFQNQASHSTRLNETKKVLFSKSNLEKTSESD
                     KSPYIQNKQEKKIFPYILMSVGAFLTLGFVIFSIHKGRRTKNESARKSNI"
     gene            3903..4145
                     /gene="esaB"
                     /locus_tag="PPY92_000923"
     CDS             3903..4145
                     /gene="esaB"
                     /locus_tag="PPY92_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaB"
                     /protein_id="HDJ2624941.1"
                     /translation="MNQHVKVTFDFTNYNYGTYDLAVPAYLPIKNLIALVLDSLDISI
                     FDVNTQIKVMTKGQLLVENDGLIDYQIADGDILKLL"
     gene            4158..5492
                     /gene="essB"
                     /locus_tag="PPY92_000924"
     CDS             4158..5492
                     /gene="essB"
                     /locus_tag="PPY92_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HDJ2624942.1"
                     /translation="MVKNHNPKNEMQDMLTPLDAEEAAKTKLRLDMREIPKSSIKPEH
                     FHLMYLLEQHSPYFIDAELTELRDSFQIHYDINDNHTPFDNIKSFTKNEKLRYLLNIK
                     NLEEVNRTRYTFVLAPDELFFTRDGLPIAKTRGLQNVVDPLPVSEAEFLTRYKALVIC
                     AFNEKQSFDALVEGNLELHKGTPFETKVIEAATLDLLTAFLDEQYQKQEQDYSQNYAY
                     VRKVGHTVFKWVAIGMTTLSVLLIAFLAFLYFSVMKHNERIEKGYQAFVKDDYTQVLN
                     TYDDLDGKKLDKEALYIYAKSYIQTNKQGLEKDKKENLLNNVTPNSNKDYLLYWMELG
                     QGHLDEAINIATYLDDNDITKLALINKLNEIKNNGDLSNDKRSEETKKYNDKLQDILD
                     KEKQVKDEKAKSEEEKAKAKDEKLKQQEENEKKQKEQAQKDKEKRQEAERKK"
     gene            5514..9953
                     /gene="essC"
                     /locus_tag="PPY92_000925"
     CDS             5514..9953
                     /gene="essC"
                     /locus_tag="PPY92_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HDJ2624943.1"
                     /translation="MHKLIIKYNKQLKMLNLRDGKTYTISEDERADITLKSLGEVIHL
                     EQNNQGTWQANHTSINKVLVRKGDLDDITLQLYTEADYASFAYPSIQDTMTIGPNAYD
                     DMVIQSLMNAIIIKDFQSIQESQYVRIVHDKNTDVYINYELQEQLTNKAYIGDHIYVE
                     GIWLEVQADGLNVLSQNTVASSLIRLTQEMPHAQADDYNTYHRSPRIIHREPTDDIKI
                     ERPPQPIQKNNTVIWRSIIPPLVMIALTVVIFLVRPIGIYILMMIGMSTVTIVFGITT
                     YFSEKKKYNKDVEKREKDYKAYLDNKSKEINKAIKAQRFSLNYHYPTVAEIKDIVETK
                     APRIYEKTSHHHDFLHYKLGIANVEKSFKLDYQEEEFNQRRDELFDDAKELYEFYTDV
                     EQAPLINDLNHGPIAYIGARHLILEELEKMLIQLSTFHSYHDLEFLFVTREDEVETLK
                     WARWLPHMTLRGQNIRGFVYNQRTRDQILTSIYSMIKERIQAVRERSRSNEQIIFTSQ
                     LVFVITDMSLIIDHVILEYVNQDLSEYGISLIFVEDVIESLPEHVDTIIDIKSRTEGE
                     LITKEKELVQLKFTPENIDNVDKEYIARRLANLIHVEHLKNAIPDSITFLEMYNVKEV
                     DQLDVVNRWRQNETYKTMAVPLGVRGKDDILSLNLHEKAHGPHGLVAGTTGSGKSEII
                     QSYILSLAINFHPHEVAFLLIDYKGGGMANLFKDLVHLVGTITNLDGDEAMRALTSIK
                     AELRKRQRLFGEHDVNHINQYHKLFKEGVATEPMPHLFIISDEFAELKSEQPDFMKEL
                     VSTARIGRSLGIHLILATQKPSGVVDDQIWSNSKFKLALKVQDRQDSNEILKTPDAAD
                     ITLPGRAYLQVGNNEIYELFQSAWSGATYDIEGDKLEVEDKTIYMINDYGQLQAINKD
                     LSGLEDEETKENQTELEAVIDHIESITTRLEIEEVKRPWLPPLPENVYQEDLVETDFR
                     KLWSDDAKEVELTLGLKDVPEEQYQGPMVLQLKKAGHIALIGSPGYGRTTFLHNIIFD
                     VARHHRPDQAHMYLFDFGTNGLMPVTDIPHVADYFTVDQEDKIAKAIRIFNDEIDRRK
                     KILSQYRVTSISEYRKLTGETIPYVFILIDNFDAVKDSPFQEVFENMMIKMTREGLAL
                     DMQVTLTASRANAMKTPMYINMKTRIAMFLYDKSEVSNVVGQQKFAVKDVVGRALLSS
                     DDNVSFHIGQPFKHDETKSYNDQINDEVSAMTEFYKGETPNDIPMMPDEIKYEDYRES
                     LSLPDIVANGALPIGLDYEGVTLQKIKLTEPAMISSENPREIAHIAEIMMKEIDILNE
                     KYAICIADSSGEFKAYRHQVANFAEEREDIKAIHQLMIEDLKQREMDGPFEKDSLYII
                     NDFKTYIDCTYIPEDDVKKLITKGPELGLNILFVGIHKELIDAYDKQIDVARKMINQF
                     SIGIRISDQQFFKFRFIQREPVIKENEAYMVANQAYQKIRWFK"
     gene            9983..10375
                     /gene="esaC"
                     /locus_tag="PPY92_000926"
     CDS             9983..10375
                     /gene="esaC"
                     /locus_tag="PPY92_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion substrate EsaC"
                     /protein_id="HDJ2624944.1"
                     /translation="MNFNDIETMVKSKFKDIKKHAEEIAHEIEVRSGYLRKAEQYKRL
                     EFNLSIALDDVESTAKDVQTAKSSANKDSVSVKGKAPNTLYIEKRNLMKQKLEMLGED
                     IDKNKESLQKAKGIAGEKASEYFNKAMN"
     gene            10391..10705
                     /gene="esxB"
                     /locus_tag="PPY92_000927"
     CDS             10391..10705
                     /gene="esxB"
                     /locus_tag="PPY92_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxB"
                     /protein_id="HDJ2624945.1"
                     /translation="MGGYKGIKADGGKVNQAKQLAAKIAKDIEACQKQTQQLAEYIEG
                     SDWEGQFANKVKDVLLIMAKFQEELVQPMADHQKAIDNLSQNLAKYDTLSIKQGLDRV
                     NP"
     gene            10705..11379
                     /locus_tag="PPY92_000928"
     CDS             10705..11379
                     /locus_tag="PPY92_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5081 family protein"
                     /protein_id="HDJ2624946.1"
                     /translation="MKDVKRIDYFSYEELTILGGSKLPLVNFELFDPSNFEEAKVALI
                     EKELVTENDKLTDAGFKVATLVREYISAIVNIRINDMYFAPFSYEKDEYILLSRFKNN
                     GFQIRIINKDIAWWSIVQSYPLLMRQEKSNDWDFKQIDDEILENLNNESIDTIGRVLE
                     IEIYNHQGDPQQSLYNIYEQNDLLLIRYPLKDKVLNVHIGVINTFIRELFGFDTDENH
                     INKAEE"
     gene            11379..11696
                     /locus_tag="PPY92_000929"
     CDS             11379..11696
                     /locus_tag="PPY92_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624947.1"
                     /translation="MTLSGKISVKAETIAHVVKELESISQKYDEIAQNFGKIAQLNYY
                     SSEKAAHSMENGYSSAATVISGLKGPLSTLGGGVMNSAQKFFEADEHWGTEFAKLYYN
                     IEG"
     gene            11706..13550
                     /locus_tag="PPY92_000930"
     CDS             11706..13550
                     /locus_tag="PPY92_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease YeeF family protein"
                     /protein_id="HDJ2624948.1"
                     /translation="MTKDIEYLTADYDNEKSSIQSVIDAIEGQDFLDVDTTMDDAVSD
                     VSSLDEDGAISLTSSVVGPQGSKLMGYYQNELYDYASQLDSKMKEIIDTPFIEDIDKA
                     FKGITNVKLENILIKNGGGHGRDTYGASGKIAKGDAKKSDSDVYSIDEILKSDQEFVK
                     VIDQHYKEMKKEDKKLSKSDFEKMMTQGASCDYMTVAEAEEIEEQKKKEEAIEIAALA
                     GMVVLSCINPVAGAVAIGAYSAYSAANAATGKNIVTGRKLSKEERIMEGLSLIPLPGM
                     GFLKGAGKSLMKLGFKGGEKFAVKTGLQKTMQQAVSRISPKMGMMKNSVLNQSRNFAQ
                     NTHVGQMLSNMRGQATHTVQQSRNWIGQQAQNVKRIVNNGLDKEIAHPFKQQLAPAGM
                     GGIKFAETTTLRNMGQNMKRAVTPQNHVTHGPKDSMVRSEGKHSVSSHEINLSKYVES
                     PNYTKVKYGEQYARLRPKKLKANIEYTTPTGHIYRTDHKGRIKEVYVDNLSLKDGGRN
                     NHAQRTVGGEDRLPDDDGGHLIARMFGGSKDIDNLVAQSKYINRSFKENGDWYKLEKL
                     WEKAIKSGKSIENIKIEVKYKGNSQRPTIFKVEYEINGKRNIRRILNK"
     gene            13561..14052
                     /locus_tag="PPY92_000931"
     CDS             13561..14052
                     /locus_tag="PPY92_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDJ2624949.1"
                     /translation="MTFEEKLSKIYNEIANEISNMIPVEWEKVYTMAYIDDGGGEVFF
                     NYTKPGSDDLNYYTDIPKEYNISVQVFDDLWMDLYDLFEELRDLFKEEDLEPWTSCEF
                     DFTREGKLKVSFDYIDWINTEFDQLGRENYYMYKKFGVIPEMEYEMEEVKQIEQYIKE
                     QEE"
     gene            14255..14938
                     /locus_tag="PPY92_000932"
     CDS             14255..14938
                     /locus_tag="PPY92_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HDJ2624950.1"
                     /translation="MTTKENIDTLRKPGAQALSLISLFLILFSCLTFFFGLDYERFPN
                     YLKITTIIELIIIVISLLQWIRFIDFEKESARKYKKIYARFLVIINVLTTITVVFALC
                     NLYYFAAVQNHYDLFNYWLMGTISIIISYLLLVIGGMFTLLKLPKVTKRWGGKTKTHF
                     GLLLTALSSFIYIEKIIEYILIPNVVESKFIIIVSMLVIAGAQFVAFQFIMQYSRFYI
                     FELNTEDDD"
     gene            15084..>15683
                     /locus_tag="PPY92_000933"
     CDS             15084..>15683
                     /locus_tag="PPY92_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="HDJ2624951.1"
                     /translation="MEFLLLIVVAGLYYIIYLTAVMYSEKIVVLPIIIYAILFVIIGI
                     TYIFIGDSYDQLTNFNVILYMGSLFYAWMAIRNLWNRPLLLKYKNITDSSSGIVNKSE
                     YNSVESLRINIEIAKYKGIISLIVAIVLTVLMTLKSTPQITAETRDLSISFFILSLFI
                     IIIFAVWDLFIRVRKGAFAFVVIRPILFSCWIFILNMILS"
BASE COUNT         6077 a         2106 c         2937 g         4563 t
ORIGIN      
        1 actttttcaa aaaatagtgt ccctaaaaag ttttgataaa cttaaaatat tcaggaggtt
       61 tctagttatg gcaatgatta agatgagtcc agaggaaatc agagcaaaat cgcaatctta
      121 cgggcaaggt tcagaccaaa tccgtcaaat tttatctgat ttaacacgtg cacaaggtga
      181 aattgcagcg aactgggaag gtcaagcttt cagccgtttc gaagagcaat tccaacaact
      241 tagtcctaaa gtagaaaaat ttgcacaatt attagaagaa attaaacaac aattgaatag
      301 cactgctgat gccgttcaag aacaagacca acaactttct aataatttcg gtttgcaata
      361 agcattctga aattggcaaa gtcacatttt ctaatgtggc tttgcttatc attttttaag
      421 aaaacaactg aaaggaaata agcatgaaaa agaaaaattg gatttatgca ttaattgtca
      481 ctttaattat tataattgcc atagttagta tgatattttt tgttcaaaca aaatatggag
      541 atcaatcaga aaaaggatcc caaagtgtaa gtaataaaaa taataaaata catatcgcaa
      601 ttgttaacga ggatcaacca acgacatata atggtaaaaa agttgagctg ggtcaagcat
      661 ttattaaaag gttagcaaat gagaaaaact ataaatttga aacagtaaca agaaacgttg
      721 ctgagtctgg tttgaaaaat ggtggatacc aagtcatgat tgttatccca gaaaactttt
      781 caaaattggc aatgcaatta gacgctaaaa caccatcgaa aatatcgcta cagtataaaa
      841 cagctgtagg acaaaaagaa gaagtagcta aaaacacaga aaaagttgta agtaatgtac
      901 ttaacgactt taacaaaaac ttagtcgaaa tttatttaac aagcatcatt gataatttac
      961 ataatgcaca aaaaaatgtt ggcgctatta tgacgcgtga acatggtgtg aatagtaaat
     1021 tctcgaatta cttattaaat ccaattaacg acttcccgga attatttaca gatacgcttg
     1081 taaattcaat ttctgcaaac aaagacatta caaaatggtt ccaaacatac aataaatcaa
     1141 tattgagtgc gaattcagat acgttcaggg tgaacacaga ttataatgtt tcgactttaa
     1201 ttgaaaaaca aaattcatta tttgacgagc acaatacagc gatggataaa atgttacaag
     1261 attataaatc gcaaaaagat agtgtggaac ttgataacta tatcaatgca ttaaaacaga
     1321 tggacagcca aattgatcaa caatcaagta tgcaagatac aggtaaagaa gaatataaac
     1381 aaactgttaa agaaaactta gataaattaa gagaaatcat tcaatcacaa gagtcaccat
     1441 tttcaaaagg tatgattgaa gactaccgta agcaattaac agaatcactg caagatgagc
     1501 ttgcaaataa caaagactta caagatgcgc taaatagcat taaaatgaac aatgctcaat
     1561 tcgctgaaaa cttagaaaaa caacttcatg atgatattgt caaagaacct gatacagata
     1621 caacatttat ctataacatg tctaaacaag actttatagc tgcaggttta aatgagggtg
     1681 aagctaataa atacgaagca attgtcaaag aagcaaaacg ttataaaaac gaatataatt
     1741 tgaaaaaacc gttagcagaa cacattaatt taacagatta cgataaccaa gttgcgcaag
     1801 acacaagtag tttgattaat gatggtgtca aagtgcaacg tactgaaacg attaaaagta
     1861 atgatattaa tcaattaact gttgcaacag atcctcattt taactttgaa ggcgacatta
     1921 aaattaatgg taaaaaatat gacattaagg atcaaagtgt tcaactcgat acatctaaca
     1981 aggaatataa agttgaagtc aatggcgttg ctaaattgaa aaaggatgct gagaaagatt
     2041 tcttaaaaga taaaacaatg catttacaat tgttatttgg acaagcaaat cgtcaagatg
     2101 aaccaaatga taagaaagca acgagtgttg tggatgtaac attgaatcat aaccttgatg
     2161 gtcgcttatc gaaagatgca ttaagccagc aattgagtgc attatctagg tttgatgcac
     2221 attataaaat gtacacagat acaaaaggca gagaagataa accattcgat aacaaacgtt
     2281 taattgatat gatggttgac caagttatca atgacatgga aagtttcaaa gacgataaag
     2341 tagctgtgtt acatcaaatt gattcaatgg aagaaaactc agacaaactg attgatgaca
     2401 ttttaaataa caaaaagaat acaacaaaaa ataaagaaga tatttctaag ctgattgatc
     2461 agttagaaaa cgttaaaaag acttttgctg aagaaccaca agaaccaaaa attgacaaag
     2521 gcaaaaatga tgaattcaac acaatgtctt ctaacttaga taaagaaatt agcagaattt
     2581 ctgaaaagag cacgcaattg ctatctgata cacaagaatc aaaaacaatt gcagattcag
     2641 ttagtggaca attaaatcaa ttagataata atgtgaataa actacatgcg acaggtcgag
     2701 cattaggcgt aagagcgaat gatttgaacc gtcaaatggc taaaaacgat aaagataatg
     2761 agttattcgc taaagagttt aaaaaagtat tacaaaattc taaagatggc gacagacaaa
     2821 accaagcatt aaaagcattt atgagtaatc cggttcaaaa gaaaaactta gaaaatgttt
     2881 tagctaataa tggtaataca gaggtgattt caccgacatt attcgtatta ttgatgtatt
     2941 tactatcaat gattacagca tatattttct atagttatga acgtgccaaa ggtcaaatga
     3001 atttcattaa agatgattat agtagtaaaa accatctttg gaataatgtc attacgtcag
     3061 gtgttattgg tacaactggt ttggtagaag gattaattgt cggtttaatt gcaatgaata
     3121 agttccatgt attagctggc tatagagcga aattcatctt aatggtgatt ttaactatga
     3181 tggtctttgt acttattaat acgtatttac taagacaggt aaaatctatc ggtatgttct
     3241 taatgattgc tgcattgggt ctatactttg tagctatgaa taatttgaaa gcggctggac
     3301 aaggtgtgac taataaaatt tcaccattgt cttatatcga taacatgttc ttcaattatt
     3361 taaatgcaga gcatcctata ggcttggcac tagtaatatt aacagtactt gtgattattg
     3421 gttttgtact gaacatgttt ataaaacact ttaagaaaga gagattaatc taatgttgat
     3481 gaatagcgtg attgctttaa cttttttaac agcatctagc aataatggcg gacttaatat
     3541 tgatgtgcaa caagaagagg aaaagcgaat caataatgat ttaaatcaat atgatacaac
     3601 gctatttaat aaagatagta aagaagtcaa cgacgcgatt gctaagcaga aaaaagaacg
     3661 acaacaacaa ataaaaaatg atatgtttca aaatcaagcg agtcactcga ctcgcttgaa
     3721 tgaaactaaa aaagtgttat tttccaaatc taacttagaa aagacttcgg agagtgataa
     3781 aagcccctat attcaaaaca agcaggagaa aaaaatattc ccgtacattt tgatgtctgt
     3841 aggggctttt ttgactttag gatttgtcat tttttcaatt cataaaggga gacgaacgaa
     3901 aaatgaatca gcacgtaaaa gtaacatttg attttactaa ttataattac ggcacatatg
     3961 acttagcagt accagcatat ttaccgataa aaaatttaat agctttagta ttggatagtt
     4021 tggacatttc aatatttgat gtcaatacac aaattaaagt gatgacgaaa ggtcaattac
     4081 ttgttgaaaa tgatggactc attgattatc aaatcgctga tggagatatt ttgaagttac
     4141 tataggagga aaaatagatg gttaaaaatc ataaccctaa aaatgaaatg caagatatgt
     4201 taacgccttt agatgctgaa gaagcagcta aaacaaaatt acgcttagat atgagagaga
     4261 ttcctaagtc ttcaattaaa ccagaacatt ttcatttaat gtacttatta gaacaacatt
     4321 ctccatattt tatagatgct gaattaactg aactacgtga cagtttccaa atacattatg
     4381 acattaatga caatcataca ccttttgata atattaaatc atttactaaa aatgaaaaat
     4441 tacgatactt actcaatatc aaaaatttag aagaagtaaa tcgtacacgt tacacatttg
     4501 ttttggcacc agatgagtta tttttcacaa gagatggatt accaattgct aaaacaagag
     4561 gtttacaaaa tgttgttgat ccattaccgg tgtcagaagc tgaattttta acaagatata
     4621 aagcactggt tatctgtgca ttcaatgaga aacaatcatt tgatgcttta gttgaaggaa
     4681 acttagaact acataaagga acgccatttg aaactaaagt tattgaagcg gcaacgttag
     4741 atttactaac ggcattttta gatgaacagt atcagaaaca agaacaagat tatagtcaaa
     4801 attatgcata tgtacgcaaa gtagggcata ccgttttcaa atgggttgct atcggcatga
     4861 caacgttaag tgtgttatta attgcatttt tagccttttt atatttttca gtaatgaagc
     4921 ataatgagcg cattgaaaaa ggataccaag catttgtaaa ggatgattat acgcaagtac
     4981 taaatacgta tgatgattta gatggaaaaa aattagataa agaggcactt tacatttatg
     5041 ccaaaagtta tatccaaaca aataaacaag gtttagaaaa agataagaaa gaaaatttac
     5101 ttaataacgt gacaccaaat tcaaacaaag actacttatt atattggatg gaattaggac
     5161 aaggacatct tgatgaagcg attaatattg ccacttattt agatgataac gatattacaa
     5221 agttagcgtt gattaataaa ttaaatgaga ttaaaaataa cggagattta tcgaatgata
     5281 aacgttctga agaaacgaaa aagtataacg ataaattgca agatatttta gacaaagaaa
     5341 aacaagttaa agatgaaaaa gcgaaatctg aagaagagaa agcaaaagcg aaagatgaga
     5401 aattaaagca acaagaagag aacgaaaaga aacaaaaaga acaagcacaa aaagataaag
     5461 aaaaacgcca agaagctgaa agaaaaaaat agtataggac tgaggcaaag acaatgcata
     5521 aattgattat aaaatataac aaacaattga agatgctcaa tttgcgagat ggtaagacat
     5581 atactattag cgaagacgag cgtgcagata ttacgttgaa atcgttaggc gaagtcattc
     5641 atttagaaca aaataatcaa ggtacttggc aagcgaatca tacttctatt aataaggtgc
     5701 ttgttagaaa aggtgacctt gatgacatta cattacagct ttatacagaa gctgattatg
     5761 catcatttgc gtatccttca attcaagata cgatgacaat tgggccaaat gcgtatgatg
     5821 atatggttat tcaaagcttg atgaatgcca tcattattaa agattttcaa tcaatacaag
     5881 aatcacaata cgtacgcatt gtgcacgata aaaatacaga tgtgtatatt aactatgaac
     5941 tacaagagca attaacaaac aaagcttaca ttggtgatca tatttatgtt gaagggatat
     6001 ggctcgaagt acaagctgat ggtttaaatg tattgagtca gaatacagtg gcatcgtcat
     6061 taattcgctt aacacaagag atgccacatg cacaggcaga tgattacaat acgtaccatc
     6121 gttcgccaag gattattcac cgtgaaccga ccgatgatat taagattgaa agaccgccac
     6181 agccaataca gaagaacaat acagtgatat ggcgttccat tataccgcca ttagtaatga
     6241 ttgctttaac tgttgtcatc tttttagtga gaccaattgg tatttatatt ttaatgatga
     6301 ttggtatgag tacagtaacg atagtatttg gtattacaac gtatttctct gaaaagaaaa
     6361 agtataacaa agatgttgaa aaacgagaga aagattacaa agcttatttg gataataaat
     6421 ctaaagaaat taataaagcg attaaagcac aacgttttag tttgaattac cattatccaa
     6481 cggttgctga aattaaagat atcgttgaaa cgaaagcacc aagaatatat gaaaaaacat
     6541 cgcatcatca cgatttctta cattataagt taggtattgc gaatgtagaa aagtcattca
     6601 aattagatta ccaagaagaa gaatttaacc aacgtcgtga tgaactattc gacgatgcta
     6661 aagaattgta tgaattttac acagatgtag aacaagcacc attaatcaat gatttaaatc
     6721 atggaccaat tgcatatatt ggtgcacgac atctcatttt agaagaattg gagaaaatgc
     6781 taatccaatt gtcaacgttc catagttatc atgatttaga gtttctattt gtgacacgtg
     6841 aagatgaagt tgaaacattg aaatgggcac gttggttgcc acatatgaca ttgagaggtc
     6901 aaaacattag agggtttgtt tacaatcaac gaacacgtga ccaaatttta acgtcaattt
     6961 atagcatgat taaagaacgt atccaagctg tgcgtgaacg cagcagaagt aatgagcaaa
     7021 ttattttcac atcgcaatta gtgtttgtca ttacagatat gtcattaatt attgatcatg
     7081 tcattttaga atatgtaaac caagatttat cagaatatgg tatttcatta atctttgttg
     7141 aagatgtgat tgaaagtttg ccagagcatg tagataccat tattgatatc aagtctcgta
     7201 ctgaaggcga actgattacg aaagaaaaag aattagttca attgaaattt acacctgaaa
     7261 atattgataa cgttgataaa gaatatatcg cgagacgttt ggcgaatttg atacacgtcg
     7321 aacatttgaa aaatgcaatt cctgatagta ttacattttt agagatgtat aacgtgaaag
     7381 aagtagatca gcttgatgtg gttaatcgat ggagacaaaa cgaaacatac aaaacgatgg
     7441 cagtaccttt aggtgtaaga ggtaaagatg atattttatc attgaactta catgaaaaag
     7501 cacacgggcc acatggttta gttgctggta ccactggttc agggaaatct gagattatcc
     7561 aatcatacat tttatcttta gctattaatt ttcatccaca tgaagttgcg ttcctattga
     7621 ttgactataa aggtgggggt atggcgaact tatttaaaga tttagtccat ttagttggta
     7681 cgattacaaa cttagatggc gatgaagcga tgcgtgcctt aacatcaatc aaagccgaat
     7741 tgagaaaacg tcaacgttta ttcggagagc atgatgttaa ccatattaat caataccata
     7801 agttatttaa agaaggtgtt gcaacagaac caatgccaca tttattcatt atttccgatg
     7861 agtttgccga attaaaatca gaacaacctg attttatgaa agaacttgta tcaacggcac
     7921 gtattggacg ttcgttaggt attcatttaa tacttgcgac acaaaaacca tcgggtgttg
     7981 ttgatgacca aatttggtct aactctaaat ttaaattggc attaaaagta caagatagac
     8041 aagacagtaa tgaaatttta aaaacaccag atgcagcaga cattacatta ccaggtcgtg
     8101 cgtatttaca agttggtaat aatgaaattt atgaattatt ccaatctgca tggagtggtg
     8161 caacatatga catcgaaggc gataaattag aagttgaaga taagacgatt tacatgatta
     8221 atgactatgg tcaacttcaa gcaatcaaca aagacttgag tggacttgaa gatgaagaaa
     8281 cgaaagaaaa ccaaaccgag ttagaagcgg ttatcgatca tatcgaatct attacaacac
     8341 gattagaaat tgaagaagtt aagcgtccat ggttaccacc attaccagaa aatgtatatc
     8401 aagaagattt agtagaaaca gatttcagaa aattatggtc agatgatgca aaagaagtgg
     8461 aattaacatt aggacttaaa gacgtaccag aagaacaata tcaaggaccg atggtattgc
     8521 aattgaaaaa agctgggcac atcgcgttaa tcggaagtcc aggatatggt agaacaacgt
     8581 tcttacacaa cattattttc gatgttgcaa gacaccatcg tcctgatcaa gcacacatgt
     8641 acttgttcga tttcggtacc aatggtttga tgccagtcac agatatacca catgttgctg
     8701 attattttac agtagatcaa gaagacaaga ttgctaaggc gatacgtata tttaatgatg
     8761 aaatcgatcg tcgtaagaag attttaagtc agtatcgtgt tactagtatt tctgaatatc
     8821 gaaaattaac tggtgaaaca attccgtatg tctttattct tattgataac tttgacgcag
     8881 taaaagattc acctttccaa gaagtttttg aaaatatgat gattaaaatg acacgtgaag
     8941 ggctagcatt agacatgcaa gtaaccttaa ctgcttcaag agctaacgca atgaaaacgc
     9001 caatgtacat taatatgaaa acgcgtatcg ccatgttttt atatgataaa tcagaggtgt
     9061 cgaacgtagt agggcaacaa aaatttgcgg ttaaagatgt agtgggtcga gcattgttaa
     9121 gtagtgatga caatgtatca ttccatattg gccaaccatt taaacatgat gagactaaat
     9181 catataatga tcaaattaat gatgaagtat cggcgatgac agaattttat aaaggtgaaa
     9241 caccaaatga tattcctatg atgccagatg aaattaaata tgaagattac agagaatcat
     9301 taagcttacc agatatagtt gccaatggtg ctttaccaat tggattagat tatgaaggtg
     9361 ttacactaca aaaaattaaa ttaactgaac cagcaatgat ttcatcagaa aatccgagag
     9421 aaattgcgca tattgccgaa attatgatga aagaaattga catattaaat gaaaaatatg
     9481 cgatttgtat cgcagactca agtggagagt ttaaagctta taggcatcaa gtggctaact
     9541 ttgccgaaga aagagaagac attaaagcga ttcatcaact aatgattgaa gacttaaaac
     9601 aaagagaaat ggacggtcca tttgaaaaag attcacttta cattatcaat gattttaaaa
     9661 catatattga ttgcacgtat atcccggaag atgatgttaa aaagcttatt acaaaaggac
     9721 cagaacttgg cttgaacatt ttatttgtcg gcattcataa agaattaata gatgcttatg
     9781 ataaacagat tgatgttgca cgtaaaatga ttaaccaatt tagtataggt attcgtattt
     9841 cagaccaaca attctttaaa tttagattta ttcaacgaga acctgttatt aaagaaaacg
     9901 aagcgtatat ggtcgcgaat caagcttatc aaaagattag atggtttaaa tagtaatgaa
     9961 ttaaatagga gggaggtatg ttatgaattt taatgatatt gaaacaatgg tcaagtcgaa
    10021 atttaaagat attaaaaagc atgctgaaga gattgcgcat gaaattgaag ttcgttctgg
    10081 atatttaaga aaagctgaac aatataagcg attagaattt aatttaagta ttgcactaga
    10141 tgatgttgaa agcacagcaa aggacgtaca aactgcaaaa tctagtgcta ataaagacag
    10201 tgtatctgtt aaaggaaaag cgcccaatac gttatatatc gaaaaaagaa atttgatgaa
    10261 acaaaagctt gaaatgttgg gtgaagatat cgataaaaat aaagaatccc tccaaaaagc
    10321 taagggaatt gctggcgaaa aggcaagtga atattttaat aaagcaatga attaatattg
    10381 aggtgaagat atgggtggat ataaagggat taaagcagat ggtggcaagg tgaatcaagc
    10441 gaaacaatta gcggcaaaaa tagctaaaga tattgaagca tgtcaaaagc aaacgcaaca
    10501 gctcgctgag tatatcgaag gtagtgattg ggaaggacag ttcgccaata aggtgaaaga
    10561 tgtgttactt attatggcaa agtttcaaga agaattagta caaccgatgg ctgaccatca
    10621 aaaagcaatt gataacttaa gtcaaaatct agcgaaatac gatacattat caattaagca
    10681 aggacttgat agggtgaacc catgatgaaa gatgttaagc gaatagatta tttttcttac
    10741 gaagaattaa caattttagg tggtagtaaa ttgcctctcg taaattttga attgtttgat
    10801 ccatcaaatt ttgaagaagc taaagtggct ttaattgaaa aagaattagt aacagagaat
    10861 gacaagttaa ctgatgcagg ttttaaagtg gctacattag taagagagta tattagcgca
    10921 attgtaaata ttcgaattaa tgatatgtat tttgcaccat ttagctatga aaaagatgaa
    10981 tatattttgt taagccggtt taaaaataat gggtttcaaa tacgaattat caataaagac
    11041 attgcatggt ggtcgattgt acaatcatat cctttattga tgagacaaga aaaatccaat
    11101 gattgggact ttaaacaaat tgacgatgaa atattggaga acttaaataa tgaaagtatc
    11161 gatacgattg ggcgtgtttt agaaattgaa atctacaatc atcaaggtga ccctcaacaa
    11221 agtttatata acatttatga acaaaatgat ttgttactca ttcgataccc attaaaagat
    11281 aaagtgctga atgttcatat tggtgtcatt aatacattta tacgagaatt atttggattc
    11341 gatactgatg aaaatcatat taataaagca gaggagtaat gacgttgagt ggaaaaatta
    11401 gtgttaaagc tgaaacgatt gcacatgttg taaaagaatt ggaaagcatt agtcaaaagt
    11461 atgatgaaat agctcaaaac tttggaaaaa tagcgcaatt aaattactac agtagtgaaa
    11521 aagctgcaca ttctatggaa aatggctata gtagtgctgc aacagtcatt agtggtctca
    11581 aaggtccatt gagtacactc ggtggtggcg tcatgaattc agcacaaaag ttctttgaag
    11641 cagatgaaca ttggggtacg gaatttgcca agctttacta taatattgag ggataggtgc
    11701 atgacatgac aaaagatatt gaatatctaa cagctgatta tgacaatgaa aagtcatcta
    11761 tccaaagtgt aatagatgca atagagggtc aagacttctt agatgtagat acaacaatgg
    11821 atgatgcggt aagcgatgtc agttctttag acgaagatgg cgcaatatca ttaacaagta
    11881 gtgtagtagg tccacaagga tctaaattaa tggggtatta tcaaaatgag ctatatgatt
    11941 atgcatctca attagattcg aaaatgaaag aaattattga cacgccattt atagaagata
    12001 tagataaagc attcaaaggt ataacgaatg ttaaattgga aaatatatta ataaaaaatg
    12061 gcggtggtca tggtagagat acctatgggg cttctgggaa aattgcaaag ggagatgcca
    12121 agaaaagtga cagcgatgtt tatagcatcg atgaaatatt aaaatcggat caagaatttg
    12181 taaaagtaat tgatcagcat tacaaagaaa tgaaaaaaga agataagaaa ctatctaaga
    12241 gtgattttga aaaaatgatg actcaaggtg cttcttgtga ttacatgaca gtagctgaag
    12301 cggaagagat agaagagcaa aagaaaaaag aagaagctat agagattgca gcactagctg
    12361 gtatggtagt tttatcttgt attaatcctg ttgctggagc agtagctatt ggtgcttatt
    12421 ccgcttattc agcagcaaat gcagccacag gaaaaaatat tgtaactgga agaaagctat
    12481 ctaaagaaga acgaatcatg gaaggacttt cgcttattcc attgccaggt atgggcttcc
    12541 tcaaaggtgc tgggaaaagt ttaatgaaat taggcttcaa aggcggagaa aaatttgcag
    12601 ttaaaacagg attgcaaaag acaatgcaac aagcagttag tcgtatttca cctaaaatgg
    12661 gaatgatgaa aaacagtgtg ttgaatcaat ctcgtaactt tgctcaaaat actcatgttg
    12721 gacaaatgct gagtaacatg cgtggtcaag caactcatac tgttcaacaa agtagaaatt
    12781 ggattggaca acaagcacaa aatgtcaaac gaatagtgaa taatggactt gataaagaaa
    12841 tagcacatcc atttaaacaa caacttgcac cagcgggaat gggtggtata aaatttgctg
    12901 aaacaactac tttgagaaac atgggtcaaa acatgaaacg tgctgttaca ccacaaaatc
    12961 acgtgacaca tggtccgaaa gatagtatgg tgagaagtga aggtaaacat agtgtaagta
    13021 gccatgaaat aaatttatca aaatatgttg aatcaccaaa ctacaccaag gtcaaatatg
    13081 gagagcaata tgcaagactt agacctaaga aactaaaagc aaatatagaa tatacaacac
    13141 caactggtca catatatcgg actgatcata aagggcgcat aaaagaagtt tatgtagaca
    13201 atctttctct aaaagatggc ggtcgtaata accatgcaca aagaactgtg ggaggagagg
    13261 atagattacc agacgatgat ggaggtcact taatcgccag aatgtttggt gggtcaaaag
    13321 acatagataa ccttgtagca caaagtaaat atatcaaccg ttcatttaag gaaaatggtg
    13381 attggtacaa gttggaaaaa ctatgggaaa aggcaatcaa gtctggaaaa tcaatagaaa
    13441 atattaaaat agaagttaaa tataaaggta atagtcaaag gccgactata tttaaagttg
    13501 aatatgaaat taatggtaaa agaaatatta gaagaatatt aaataagtag aggtgccaac
    13561 atgacatttg aagagaagct tagcaaaata tacaatgaaa ttgcgaatga gattagcaat
    13621 atgataccgg tagagtggga aaaagtatat acaatggctt atatagatga tggaggaggg
    13681 gaggtattct ttaattatac taagccaggt agtgatgact tgaattatta caccgatata
    13741 cctaaggagt ataacatttc tgtgcaagta tttgatgatt tatggatgga tttatatgat
    13801 ttgtttgagg agttaagaga tttatttaaa gaagaagatt tagaaccatg gacatcatgt
    13861 gaatttgact ttacaagaga gggtaaattg aaagtatcat ttgattatat agattggata
    13921 aatacagagt ttgatcaatt gggccgtgaa aattattata tgtacaaaaa atttggggtt
    13981 ataccagaaa tggaatatga aatggaggag gttaaacaaa tcgagcaata tattaaagag
    14041 caagaagaat agacaatctt aatgtgagac taaacaataa agctttgttt agtctttttt
    14101 ggcggttaag taaaaagcaa tagataccgt aaagttgatg ctcgtcaaat aataatataa
    14161 agatagtttt atgctttgta tagataaaaa cctaagtact tataacttta aatacgtaat
    14221 atgctattta aaagataatg tataggagac agatatgact actaaagaaa atattgatac
    14281 tcttcgaaag ccaggtgcac aagccttaag tttaatatca ttgtttttga tacttttttc
    14341 atgtctaact ttcttttttg gtttagatta tgaaaggttt ccaaactatt taaagataac
    14401 gacaattata gaattaataa ttattgtaat tagtttactt caatggatta ggtttataga
    14461 tttcgaaaaa gaaagtgcac ggaaatataa aaaaatatat gctcgatttt tagttattat
    14521 aaatgtgtta actactatca ctgtagtatt tgcactgtgt aacctttatt attttgcagc
    14581 tgtacaaaac cattatgatt tattcaatta ttggttaatg ggtacgattt caatcataat
    14641 tagctattta ttattagtaa ttggcggaat gtttacgttg ttaaaattac ctaaagtaac
    14701 aaaacgctgg ggtggtaaaa ctaaaacaca tttcggttta ttattaactg cgttgagctc
    14761 atttatatat attgaaaaaa ttatcgaata tatattgatt cctaatgtcg tagaatccaa
    14821 gtttataatt attgtgagca tgttggttat cgctggagca caatttgtgg catttcaatt
    14881 tattatgcaa tacagtagat tctatatttt tgaattaaac acagaagatg atgactaaat
    14941 gattttgtaa atactgtgaa gtatacttaa attaagaaaa tcaacttcaa cgtatatcac
    15001 catattattt tatactcaat gatgtgaatg gatttaagtg gattgatatt aaagtaaaag
    15061 ttatggaata ttgaggtgcg aatatggagt tcttattatt aattgtcgta gccggactgt
    15121 attatattat atatttaact gctgtgatgt attctgaaaa aatagtagta ttgcctataa
    15181 tcatctatgc cattttgttt gtaataattg gtatcactta tatctttata ggcgacagct
    15241 atgatcaatt aacaaatttc aatgtgattt tgtatatggg gagtttgttt tatgcatgga
    15301 tggctattag aaatctttgg aacagaccac tattactaaa atataagaac attacagata
    15361 gttcaagtgg aatagttaat aaatctgaat acaattcagt tgaaagctta cgtataaata
    15421 ttgaaatagc taagtataaa gggattattt ctttgatagt agctatagta ctaacggtat
    15481 taatgacatt aaagtcaaca cctcaaatca ctgcagaaac acgtgactta agtatctcat
    15541 ttttcatact cagcttattt atcattatta tatttgcagt ttgggatttg tttattagag
    15601 ttagaaaagg agcgtttgct tttgttgtaa taaggccaat attattcagt tgttggatat
    15661 ttattttgaa tatgatttta tca
//
LOCUS       DANBWH010000042        15656 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.044, whole genome shotgun sequence.
ACCESSION   DANBWH010000042 DANBWH010000000
VERSION     DANBWH010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15656
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.044"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(85..600)
                     /locus_tag="PPY92_000934"
     CDS             complement(85..600)
                     /locus_tag="PPY92_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDJ2624952.1"
                     /translation="MAGIIKEISEQNASELVELATRTFYDTFGSYYDDKDFDQFFKDN
                     YTVEKFTQEINHVDSFHYFYQEDGANVGYIKMNINSAQTEEMGETYLEVQRIYFLKDF
                     QGGGRGSQLIELAEKIAQEHNKHKIWLGVWEHNPRAQAFYKRHGFKVVGEHHFQTGDV
                     TDTDLIMEKEL"
     gene            791..1795
                     /locus_tag="PPY92_000935"
     CDS             791..1795
                     /locus_tag="PPY92_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDJ2624953.1"
                     /translation="MIETFKAFVIDKDESGKVTPTFKQLSPTDLPKGDVLIKVHYSGI
                     NYKDALATQDHNAVVKSYPMIPGIDLAGTIVESEAPGFEKGEQVIVTSYDLGVSHYGG
                     FSEYARVKSEWIIKLPDTLTLEESMIYGTAGYTAGLAIERLEKVGMNIEDGPVLVRGA
                     SGGVGTLAVLMLNELGYKVIASTGKQDVSDQLLELGAKEVIDRLPVEDDHKKPLASST
                     WQACVDPVGGEGINYVTKRLNHSGSIAVIGMTAGNTYTNSVFPHILRGVNILGIDSVF
                     TAMKLRQRVWRRLAKDLKPENLHEIKQVITFDELPEQLNKVINHENKGRIVIDFGVDK
                     "
     gene            complement(1841..2242)
                     /locus_tag="PPY92_000936"
     CDS             complement(1841..2242)
                     /locus_tag="PPY92_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDJ2624954.1"
                     /translation="MVKVTYDIPTCEDYCALRINAGMSPKTREAAEKGLPNALFTVTL
                     YDKDRLIGMGRVIGDGGTVFQIVDIAVSKSYQGQDYGSLIMEHIMKYIKNVSVESAYV
                     SLIADYPADKLYAKFGFMPTEPDSGGMYIKY"
     gene            complement(2278..3312)
                     /locus_tag="PPY92_000937"
     CDS             complement(2278..3312)
                     /locus_tag="PPY92_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDJ2624955.1"
                     /translation="MKDVTIIGGGPSGLYASFYAGLRDMSVRLIDVQSELGGKMRIYP
                     EKIIWDIGGIAPKPCHEILKDTIKQGLYFKPEVHLNERVVDIRKKAERHFEVETEAGE
                     IYTSKAVIIAIGAGIINPKQLDVKGVERYQLTNLHYVVQSYRRFKDKDVLISGGGNTA
                     LDWAHDIAKIAKSVTVVYRKEDVSGHEAMKTLVTDLNVKLCPKTRIKYLVGNDDETHI
                     SEVVLEHVESGDRHTVKFDDVIISHGFDRCNTLLSETSSKLDMHDDCRVKGFGNTTTS
                     IPGIYACGDIVYHDAKSHLIASAFSDGANAANLAKTYIQPDANAEGYVSSHHEVFKEA
                     NKTIVNKHLY"
     gene            complement(3323..3439)
                     /locus_tag="PPY92_000938"
     CDS             complement(3323..3439)
                     /locus_tag="PPY92_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624956.1"
                     /translation="MINYAIYFNNININSIMALNDNNYQFEHVNERNEYRES"
     gene            complement(3544..3975)
                     /locus_tag="PPY92_000939"
     CDS             complement(3544..3975)
                     /locus_tag="PPY92_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2871 domain-containing protein"
                     /protein_id="HDJ2624957.1"
                     /translation="MRRLLYSFLFYMIIGLFSGFFYRELTKAYDFTGTTQLSLVHTHT
                     LILGMFMFLILLPLEKLFKLSSYYLFNWFFYVYNIGVIVTIGMMVTKGFFQVTGKSYS
                     PEAFAGFAGIGHTGMLAGLLLLFFLLRQAILKEPRDESFKK"
     gene            complement(4182..5459)
                     /locus_tag="PPY92_000940"
     CDS             complement(4182..5459)
                     /locus_tag="PPY92_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDJ2624958.1"
                     /translation="MNIFKNKLLWIAPIATMIILVIFSLAFYPAYNPKPKDLPIGILN
                     EDKGTTIQDKNVNIGKKLEDKLLDSDSNKIKWVKVDSEKDLEKDLKDQKIFGVAIIDK
                     DFSKDAMSKTQKVVMDSKKEEMQQKVASGEIPPQVVQQMKQKMGNQQVEVKQAKFKTI
                     VSEGSSLQGSQIASAVLTGMGDNINAQITKQSLETLTSQNVKVNAADINGLTNPVKVD
                     NEKLNKVKDHQAGGNAPFLMFMPIWIGSIVTSILLFFAFRTSNNIVVQHRIIASIGQM
                     IFAVVAAFAGSFVYIYFMQGVQGFDFDHPNRIAIFVAFAILGFVGLILGVMVWLGMKS
                     VPIFFILMFFSMQLVTLPKQMLPESYQKYVYDWNPFTHYATSVRELLYLNHHIELNST
                     MWMFIGFMIFGAVSSLVSAIVRKHSTKRTEVPS"
     gene            5620..6243
                     /locus_tag="PPY92_000941"
     CDS             5620..6243
                     /locus_tag="PPY92_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDJ2624959.1"
                     /translation="MKEDRRIRKTKSSIKQAFTKLLQEKDLEKITIRDITTRADINRG
                     TFYLHYEDKYMLLADMEDEYISELTTYTQFDLLRGSSIEDIANTFVNNILKNIFQHIH
                     DNLEFYHTILQLERTSQLELKINEHIKNNMQRYISINHSIGGVPEMYFYSYVSGATIS
                     IIKYWVMDKQPISVDELAKHVHNIIFNGPLRIMAENRLHKSNLDSLT"
     gene            6289..7233
                     /locus_tag="PPY92_000942"
     CDS             6289..7233
                     /locus_tag="PPY92_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDJ2624960.1"
                     /translation="MITSFRHSEDIDKHIIKTPLDHTASWINVVEPDREEIENLMEQY
                     NIPEDFIRDPLDSEESSRIEYDEDTGYSLIIIDLPIVNSTNRSVLSFVTIPLGIIIGN
                     GIIVTVCDAENEFLENLPKRDINLKFHSRFALEILTTIADHYNRNLRLLNKSRIRIEK
                     ELKNNITNKQLFKLMEVEKSLVYFLAALKGNDTIIKKLFHLPAIKRFEEDEELLEDLI
                     IENNQAIETTELHQRILESITTSYASLLSNDMNTIMKTLTLFTVLLTLPTLVFSFFGM
                     NVPLPIDDHSYISWIIVVGISLILVVIVSIFLWRKQKL"
     gene            complement(7318..8760)
                     /locus_tag="PPY92_000943"
     CDS             complement(7318..8760)
                     /locus_tag="PPY92_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HDJ2624961.1"
                     /translation="MNYKQSAEDILNAIGGEENLDAMAHCATRLRLVLNDESLVNEEA
                     LNNMDVVKGTFSTGGQYQIIIGSGTVNKVFSELEKLTGKEASTTSEVKAQSAKNMNPL
                     QRFVKMLSDIFVPIIPAIVAGGLLMGLNNILTAKDLFFSGKSLIDVYSQFAGLAEMIN
                     VFANAPFTLLPILIGFSAAKRFGGNPFLGAVLGMILVHPSLMSAYDFPKAVEAGKAIP
                     YWDVFGLHINQVGYQGQVLPMLVAAYILASIEKGLRKVIPTVLDNLLTPLLSIFITAF
                     LTFSFVGPITRQLGYWLSDSLTWLYEFGGAIGGLIFGLLYAPIVITGMHHSFIAVETT
                     LIADATKTGGSFIFPIATMSNVAQGGAAIAAFFIIKQNKKLKGVASAAGISALLGITE
                     PAMFGVNLKLRYPFIGAIVGSGIGSAYIAFFKVKAIALGTAGLPGFISINPVHAGWLH
                     YFVGMTISFIIAITVTLILSKRKANKEVVE"
     gene            8943..9326
                     /locus_tag="PPY92_000944"
     CDS             8943..9326
                     /locus_tag="PPY92_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HDJ2624962.1"
                     /translation="MKERGHIEQLWREEKYHVLLHSQQSYQMIRNALKTDLSLHQVQQ
                     MIDDALLIEPSIGSVCNAFDHMWGYFKKCANEEERKQSKLLKADFINGKIDTQTLLDF
                     LAELANKYDVQYLLQSRVLNTKRKR"
     gene            10814..12919
                     /gene="rsp"
                     /locus_tag="PPY92_000945"
     CDS             10814..12919
                     /gene="rsp"
                     /locus_tag="PPY92_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator Rsp"
                     /protein_id="HDJ2624963.1"
                     /translation="MTCQLKIHHTLSTIPSRNINNIMVLFSLTSKLNITINGETKDVS
                     NYIILINHGDIYNINHGENIIELMIPVFYFYQQDDDFFNGYLDRHLLQSSNYIKSLIA
                     DLISTPTSSSLMGKNIGQSIIDTLLKEAFIRIDHEYLPNIALSNPVFIDCVNYIHDNI
                     DAHLSLKDIAMHCNISESYCSNLFVRYLSMNFKDYFTSIKLVNAINLLLSTKHSITTV
                     SELAGFNSHTNFANQFKNYLHFSPKQFRSLVSKITEPPQIHFQQDNVSQFTELISTID
                     LTAQLATNTTDIHIDDFNPKDRSQRAKVFVRFSNFNELFQFIFNEYYDINFEHLPKPV
                     VFIDDIHDIEISQTNYNLLNRCFEKLFEKNIGLAIAIKSTQQFETMKQLILTFLQGNQ
                     DYKTSKKLVKFMLVFCSNSMTAEEIHLCHLKIKNKNKEIKYSVTVDGFLETYSTVEQV
                     YDVMQRLKFHYYFIDIENSKTATHLITKNQHYHQTDTHFEQYKKFILDSGISSTQFVY
                     NNLSVSGFKYTNDGKNPIQLSDIVYHLIALLRYGGGISYQLLDDHSNYISLYNKYGSP
                     LPLMHLYKMFRPFVNEDIEITNNYVLSRKDNNYHFLLFNKINDRYMSDVKQDFIFHNE
                     LPQDSLMIIKTLNHEHGSIQHLLPISDQLVYIEKEILDELDKTNYPKTELAVQEETGR
                     TFELKLNHDEVKYICFKPS"
     gene            12990..13412
                     /locus_tag="PPY92_000946"
     CDS             12990..13412
                     /locus_tag="PPY92_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDJ2624964.1"
                     /translation="MSNSQAIQAIENVLATSKVGVLSTAYNNKPNSRYMVFYNDGLTL
                     YTKTNIHSAKVKEIKDNPAAYVLLGYNDTTNRSFVEMEATIEVVTDQKVIDWLWETQD
                     KSFFSSKEDPELCVLKVTPQSVKLMNDKSLDTPIKIDL"
     gene            13676..14032
                     /locus_tag="PPY92_000947"
     CDS             13676..14032
                     /locus_tag="PPY92_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HDJ2624965.1"
                     /translation="MKKKKGFGLGISLIAIMLIVCIVLVIMMMTGGKKDTYYGIMKDN
                     TTIEKMISEKDESIEKNVKLPSDSDVKVKKGDFVIVYKLADSDKIVKVKKVDHDDVPH
                     GLMMKIHDMGKMHMKH"
     gene            complement(14350..15627)
                     /locus_tag="PPY92_000948"
     CDS             complement(14350..15627)
                     /locus_tag="PPY92_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HDJ2624966.1"
                     /translation="MALFKRKISLPMQVVIALVLGVVVGLLLYGQENVANYIKPFGDV
                     FLNLIKMIVIPVVFCSLALSISNVGESKTVGRYGWKTILYFEIITTIAIGLGIIFGNL
                     FKPGAGLDPTKLPKGDISKYQSTAHAAEQSTYGNHFIDTIVHIIPTNFFEALNKGELL
                     PIIFFAVFFGLGLAAVGKKAEPVKEFLSGSLEAVFWMINKILKLAPLGVFAFICTTII
                     TFGASALLPLLKLVLVVVFAMVFFVFAILGLVAWMCGINIMNIIRILKSELLLAFSTS
                     SSEAVLPVMMKKMENFGSPKEITSFVIPIGYTFNLDGSALYQSIAALFVAQMYGMHLT
                     LSEQIVLMLTLMITSKGMAAVPGTSIVVLLTTLGAMGLPAQGLALIIGVDRILDMVRT
                     CVNVIGNALSTIVIAKWENVYDKAKGQEYLKSI"
BASE COUNT         5380 a         2658 c         2281 g         5337 t
ORIGIN      
        1 aacaattcat tacgcattta gcatttcaag gcatacacaa tatttattac tatgatttca
       61 ttttatctgc tgcaaaaaca atcattataa ctctttttcc ataattaaat ctgtatccgt
      121 tacatcacct gtttgaaaat gatgttcacc aaccacttta aatccatgac gtttataaaa
      181 tgcttgagca cgaggattat gctcccaaac tcctagccaa attttatgtt tattatgttc
      241 ttgagcaatt ttttcggcca attctatcaa ttgtgaacct cttccgccac cttgaaagtc
      301 tttcaaaaaa tatatgcgct gcacttctaa ataggtctcc cccatttctt cagtttgagc
      361 actattaata ttcatcttta tataaccaac attcgcacca tcttcttgat aaaaataatg
      421 aaatgaatct acatggttaa tctcttgtgt aaatttttct acagtataat tgtctttaaa
      481 aaattgatca aaatctttgt catcatagta agaaccaaac gtgtcataaa atgttctagt
      541 tgctaattca actaattcac tagcattttg ttctgaaatt tctttgatta tcccagccat
      601 ataaatcctc caataaacag tgatcgaatc aaaatattac ttatgttatt tttcagccaa
      661 aactatttaa aaatacatta acacaaatca attacaaatt gtattgattg tgtgtaacat
      721 caataaatga tacatttatt ccagtaaaat ggccgtattt tcaaaagaga aaaagagagg
      781 atgtatcgtt gtgatagaaa catttaaagc gtttgtaatt gataaagatg agagcggtaa
      841 agtgacacca actttcaaac aattatcgcc tactgattta cctaaaggag atgtgctgat
      901 taaagtacat tactctggta taaattataa agatgcttta gcgactcaag atcataatgc
      961 agtcgtaaaa tcgtatccta tgattccagg aatagattta gctggaacaa ttgttgaatc
     1021 cgaagcacca ggctttgaaa aaggagaaca agtaattgta acgagttatg acctaggtgt
     1081 tagccattat ggcggtttta gtgaatatgc gcgtgtaaaa tcagaatgga ttatcaagct
     1141 tcctgatact ttaacattag aagaatcaat gatatatggc acagctggtt atactgccgg
     1201 tttagcaatt gaaagacttg aaaaagttgg aatgaatatt gaagatggtc ctgtactcgt
     1261 tcgcggtgct tcaggtggtg tcggtacttt agcagtactc atgcttaatg aacttggtta
     1321 taaagttatc gcaagtacag gtaaacaaga tgttagcgat caattacttg aacttggtgc
     1381 caaagaagtt atcgatcgac ttcctgttga agatgatcat aaaaagccac tcgcatcatc
     1441 aacttggcaa gcttgtgtag accctgttgg tggcgaaggt attaattatg ttacaaaacg
     1501 tttaaatcat agtggatcaa ttgcagttat tggtatgact gccggcaata cttatactaa
     1561 ttctgtattc cctcacattt taagaggcgt aaacatttta ggaattgact cagtatttac
     1621 tgctatgaaa ttaagacagc gcgtttggcg tcgtctcgca aaagatttaa agcctgaaaa
     1681 tttacatgag atcaagcaag ttattacatt tgatgaactt ccagaacaac ttaacaaagt
     1741 aattaatcat gaaaataaag gccgcattgt tatcgatttc ggtgtagata aatagtattc
     1801 atgaaaaaga catcccgtta tgcgagatgt cttttttaat ttagtatttg atatacatac
     1861 cgcctgaatc tggttcggta ggcataaatc caaattttgc atataattta tccgctgggt
     1921 agtctgcgat cagactaacg tatgcacttt cgacagatac atttttaata tacttcataa
     1981 tatgttccat aattagactg ccgtaatctt gaccttgata acttttcgaa acagcaatat
     2041 caacaatttg aaaaacagtt ccgccatcgc caatcactct acccatacca attaaccgat
     2101 ctttatcata caaggttact gtaaataagg cattaggtaa tcctttttca gctgcttcgc
     2161 gcgtctttgg actcatacct gcgttaatcc ttaatgcgca ataatcctcg caagtcggaa
     2221 tatcatatgt cactttaacc attatttacc ccacttttca tcacacaata tatcaaccta
     2281 gtataaatgt ttatttacaa tagtcttatt cgcttcttta aacacttcat gatgacttga
     2341 aacataaccc tctgcattcg catctggttg gatatatgtt ttagcaaggt tcgctgcatt
     2401 tgcaccatca ctaaatgcac ttgcaattag atgtgatttt gcatcatgat aaacaatatc
     2461 tccacacgca tagataccag gtatactagt tgtcgtatta ccaaatcctt taacacgaca
     2521 atcatcatgc atatctagct ttgaagatgt ttcactcaat aatgtattac aacgatcaaa
     2581 cccatgacta ataatgacat cgtcaaattt aactgtatgc ctatcgccac tttcaacatg
     2641 ttccaaaaca acttcactta tatgcgtttc atcatcattg ccgaccaagt atttaatacg
     2701 tgtttttggg catagtttca catttaaatc tgtcaccaac gttttcatcg cttcatgacc
     2761 acttacatct tcttttcgat aaacaactgt cacgctttta gcaatcttgg caatatcatg
     2821 cgcccaatct aatgctgtat ttcctccacc tgatattaat acatctttat ctttgaaacg
     2881 tctgtaactt tgtacaacat aatgtaaatt agttaattga tatctctcta cacctttaac
     2941 atctaattgt tttggattaa taatacccgc accaattgca atgataactg ctttcgatgt
     3001 atatatttct cccgcttctg tttcaacttc gaaatgacgt tctgcctttt tcctaatatc
     3061 taccacacgt tcattcaaat gaacttccgg tttaaaatat aatccttgct taattgtatc
     3121 ttttaaaatt tcatgacaag gttttggcgc aatgccgcca atatcccaaa taattttttc
     3181 agggtaaatt ctcatcttac cccctaattc agattgaaca tctatcaatc ttacagacat
     3241 atctcgcaat ccagcataaa agcttgcata caaaccagac ggaccgccac caatgattgt
     3301 aacatctttc attatgtgcc tcctatgact ctctatattc atttctttca ttaacgtgct
     3361 caaattgata attattatca tttaaagcca ttatactatt aatatttata ttgttaaaat
     3421 aaatcgcata gttaatcatg aattatcaat aactatccca tcacaatata aaaacacacc
     3481 tttcaaagta agaaacaaca atgttccttt gaaagatgtg tatatttttt agttctagtt
     3541 atattatttt ttaaaagact catcacgtgg ttctttaaga attgcttgtc ttaaaaggaa
     3601 aaatagcaac aataaacctg caagcatacc tgtgtgccca atacctgcaa agcctgcaaa
     3661 tgcttctgga gagtatgatt taccagtgac ttggaagaat ccttttgtca ccatcatacc
     3721 tatagtaacg ataacaccta tgttatatac atagaagaac cagttaaata agtagtaact
     3781 tgataattta aataacttct ccaatggtaa taatattaaa aacataaaca tacctaaaat
     3841 aagtgtatgt gtgtgtacaa gtgataattg tgttgtacca gtaaaatcat atgcttttgt
     3901 tagctctcta tagaaaaagc cactaaataa acctatgatc atataaaaaa gaaatgaata
     3961 taataaccta cgcattttga atcctccctc accttttcat tatatccgat tatacctatt
     4021 ataacagtga caaaaaagcg aagatatgac acaactgaat ttggtttagc acatagtatg
     4081 acagctaaaa taactttaca tcaagcgcta tacaaataaa atagctatac catgacgctc
     4141 gctacaaacg tcttgtatag ctattatttc tatttattaa attatgacgg cacttcagta
     4201 cgttttgtgc tatgttttct aacaatagct gaaactaaac ttgatacagc gccaaaaatc
     4261 ataaacccta taaacatcca cattgtacta tttaattcaa tatgatgatt caagtataat
     4321 agttctctta cacttgttgc atagtgtgtg aatggattcc aatcatatac atatttttga
     4381 taactttcag gcaacatttg tttaggtaac gttacaagtt gcatactaaa gaacattaaa
     4441 atgaagaaaa ttggaactga cttcatacct agccatacca taacacctaa aataaggccc
     4501 acgaaaccaa gaatcgcaaa tgctacaaaa attgcgatac gatttggatg gtcaaaatca
     4561 aatccttgaa cgccttgcat gaaataaata taaacaaagc tacctgcaaa tgctgcaaca
     4621 actgcaaata tcatctgtcc aattgaagca atgatacgat gttgcacgac gatattgtta
     4681 ctagttctaa atgcaaagaa caataagata gacgttacga ttgaacctat ccaaattggc
     4741 ataaacatta ggaatggtgc attaccacct gcttggtgat ctttaacttt attaagtttt
     4801 tcattatcca cttttactgg attcgtcaaa ccattgatgt ccgcggcatt gactttaaca
     4861 ttttgactcg ttaatgtttc caaactttgc ttcgtaattt gagcattaat attatcaccc
     4921 ataccagtta acacagctga tgcaatttgt gaaccttgta agcttgatcc ttcacttaca
     4981 atcgttttaa atttagcctg cttaacctct acttgttgat tccccatttt ttgtttcatt
     5041 tgttgaacca cttgtggcgg aatttcacct gaagcaactt tttgttgcat ttcttctttt
     5101 ttactatcca taactacttt ttgtgtttta ctcatagcat cttttgaaaa gtctttatca
     5161 ataatagcta ctccaaagat tttttgatct ttcaaatctt tttcaaggtc tttttcacta
     5221 tcaaccttaa cccatttaat tttattagaa tcactatcta ataatttatc ctctaatttt
     5281 ttaccaatgt taacattttt atcttgaatc gttgtacctt tatcctcgtt taatatacca
     5341 attggtaaat cttttggttt aggattatat gcaggataaa aagctaaaga aaagattacc
     5401 aagataatca tagttgctat tggcgcaatc cataataatt tatttttaaa tatattcatt
     5461 ttattaatta cctccttatt agacaatgtg ttgaatattt cgcatgttgt ctattataat
     5521 atgtaagtgg gaataaacaa tacacaatgt tcatcaatac gttcattaat agacaaatac
     5581 acccacattg ttcaataaca caaggaggtt gatttatttt tgaaagaaga taggcgaatt
     5641 agaaagacaa agtcctccat taaacaagcg ttcactaaat tactacaaga aaaagattta
     5701 gaaaagatta ccattcgcga cataacaaca cgcgctgata tcaatagagg gacattttac
     5761 ttacattacg aggataaata tatgttactc gcagatatgg aagatgagta tatttcagaa
     5821 ctaacgacat atactcaatt tgatttgtta cgcggttctt caattgaaga cattgcgaat
     5881 acttttgtaa ataatatact caaaaatatt ttccaacata ttcatgataa tttagagttt
     5941 tatcatacta ttttacaatt ggaacgcacg agtcaattag aattgaaaat caacgaacat
     6001 atcaaaaata atatgcaacg ttatattagt atcaatcatt ctatcggagg cgttccagag
     6061 atgtattttt acagttacgt ttccggagca acaatttcaa ttattaaata ctgggtaatg
     6121 gacaaacagc ccatttcagt tgatgaatta gctaaacatg tgcataatat tatttttaat
     6181 ggtccattaa gaataatggc agaaaaccgt ttgcataaat caaaccttga ttcactaact
     6241 taagtaatac tcgcttttcc taaatccttt agaaaggaac tatatatcat gattacttca
     6301 ttcagacatt ctgaagatat agataaacat attataaaaa caccattaga tcatacagcg
     6361 tcatggatta atgtagtaga accagaccga gaagaaattg aaaatcttat ggaacaatat
     6421 aatatacctg aagactttat acgtgaccct ttggactcag aagaaagttc ccgtattgaa
     6481 tatgacgaag atactggtta ctcattaatc attattgatt tacctatcgt caattcaact
     6541 aatcgtagcg ttctatcttt tgtaacgatt ccattaggca ttattattgg caacggtatt
     6601 atcgtaacag tttgtgacgc tgaaaatgaa tttttagaaa atttacctaa gcgtgatatt
     6661 aatttaaaat ttcacagcag atttgcatta gaaattttaa ctactattgc cgaccattat
     6721 aatcgtaact tacgattact taataaaagt agaattcgta ttgagaaaga actaaaaaat
     6781 aacattacta acaaacaact tttcaaatta atggaagttg aaaaaagttt agtatacttt
     6841 ttagctgcct taaaaggtaa cgatacaatt attaaaaagt tattccattt acctgcaatt
     6901 aaacgttttg aagaagatga agaattacta gaagatttaa tcattgaaaa taaccaagcc
     6961 atcgaaacaa cagaattaca tcaacgtatc ttagaaagta tcacaacatc atacgcttcc
     7021 ttattatcta atgatatgaa tacgattatg aagacattaa cacttttcac ggtactatta
     7081 acgttaccaa cgctcgtatt tagtttcttc ggtatgaatg tgccgttgcc aattgatgac
     7141 catagttata tatcttggat tattgtggta ggtatttcat taattcttgt cgtaattgtt
     7201 agtattttct tatggcgtaa acagaaatta taactaaatt aaaaggtatg acagcttaaa
     7261 gtttcatcct attgcatttg caatgactga tactttgatt actgtcatac ctctttttta
     7321 ttctacaact tctttatttg cttttctttt agataaaatt aaagtaactg ttatagcaat
     7381 gatgaatgat attgtcatac caacaaagta gtgtaaccat cctgcatgta ctggattgat
     7441 tgaaataaat cctggcaatc cagcagttcc taatgcgatt gctttaacct tgaagaaagc
     7501 aatatatgct gaaccaatac ctgatccaac gatagcgcca ataaatggat atcttagttt
     7561 taagttaaca ccaaacatag ccggttctgt aataccaagt aatgctgaaa tacctgcggc
     7621 agatgccaca ccttttaact tcttattttg tttaataata aagaacgctg caattgctgc
     7681 accaccttgt gcaacattag acattgtcgc aatcgggaat ataaatgatc cacccgtttt
     7741 agtggcatct gcaattaatg tcgtttctac agctataaag ctatgatgca tacctgtaat
     7801 aacaatcgga gcatacaata atccgaatat taatccacca attgcaccac caaattcata
     7861 aagccatgtt aaactatctg ataaccagta acctaattgt cgagtgattg gacctacaaa
     7921 tgaaaatgtt agaaatgctg ttataaaaat agataacaat ggtgttaaca aattatctaa
     7981 caccgttgga ataactttgc gtaacccttt ttcaattgag gctaagatat aagctgctac
     8041 aagcataggt aacacttgtc cttgataacc tacttgatta atatgcaaac caaaaacatc
     8101 ccaatatgga atagccttac ctgcttcaac tgcttttggg aaatcgtatg cgctcattag
     8161 cgatggatga actagtatca tacctaatac agcacctaaa aatggattgc caccaaagcg
     8221 ttttgctgca ctaaatccaa ttaaaattgg taataatgta aatggtgcat tcgcaaaaac
     8281 atttatcatt tcagctaatc cagcaaattg actatataca tctatcaatg atttacctga
     8341 aaagaataaa tctttcgcag tcaaaatgtt atttaacccc attaataaac caccagcaac
     8401 gatggctggt ataatcggaa caaagatatc tgaaagcatt tttacaaatc gctgtaacgg
     8461 attcatattt ttagcagatt gtgctttgac ttccgaagtg gttgatgctt cttttccagt
     8521 taatttttcc agttcactaa atactttatt gactgtacca gacccaataa taatttggta
     8581 ttgtccccca gtagaaaacg tccctttaac tacatccata ttgtttagcg cctcttcatt
     8641 tactaaactt tcatcattta aaactaatcg tagtctcgtt gcacaatgcg ccattgcatc
     8701 taaattctct tctccgccta tcgcgttcaa aatatcttct gcggattgct tataattcat
     8761 atatttagcc tcctaattag aaccggttcc atttattcca ctttattgta accggttcca
     8821 aaaatatttg caacactcta ttatcatttt atataaacac ctacaccatt tttgatagcg
     8881 cattcatttt tattgctata ctatatgcat gtatattaac attaattatc ggaggtaatt
     8941 ttatgaaaga acgtggccat atcgaacagc tttggcgtga ggaaaaatat catgtattgt
     9001 tacacagtca acagtcttac caaatgataa ggaatgcttt aaaaacggat ttatcacttc
     9061 atcaagtcca acaaatgatt gatgatgctt tgttaattga accgagtata ggaagtgtat
     9121 gtaatgcttt tgatcatatg tggggatatt ttaaaaaatg tgcgaatgaa gaagaaagaa
     9181 aacaatcaaa actacttaaa gctgatttta ttaatggtaa aatcgacacc caaactttat
     9241 tagatttttt agcagaactt gctaataaat atgatgttca atatttacta caaagtcgtg
     9301 ttttaaacac aaaaagaaag agatagattg aaactttagt tgtaacggac aaatagtagt
     9361 cattggagtt cagcaattca tactttgtac taaagtttac tataatctat ctctcaaggt
     9421 ttaaatgtac atatctatat taagatgcgt ttcatgatgt tagaagatac catacacatt
     9481 taattagtta cagcataaat caatttatac ccttaattat tacattgttt atattttaaa
     9541 ttcgggggag taattttaag taatatcttg ttgctgctaa ttctattgtt aataaattga
     9601 tattagatgt agaaattgag tgtgaaagtt aataatagat gatttagcta gtaacataat
     9661 taattcatat atagtcaaaa agttctgtta gatactgttc ttgatgaata ttctttgatg
     9721 ttaggtgtta gctattctag atgagtttct tagatgtgga tgcctgtctt gatgaagtta
     9781 tctatgatgt taatataata cttgatgttg attcttagat gttagtgcga tttcaagatg
     9841 ctgattcttt gttgttgcac tatcttggtg acgtttctag ttgtttatag ttatcgcatc
     9901 tcttgatgta aaatctaaga tgtttatgca tttctagatg ttaattcttc gctgcttaag
     9961 aattatcttg atggtaaatc tagttgtgcc atgcattttc tcatgatgaa tctaaggtgt
    10021 taattcattg atgtgtgctg tttcttgttg cttcattatc ttgatggtga atttcgttgt
    10081 ctaatgcact ttttcaaatg attattctaa ttgttacggc aataactaga tggattttct
    10141 tagatgttca cttttctaga tgtttatact tatcttgatg ttttctaaac agaactttta
    10201 aacgctttat tttttagtag attgtttaaa atttcttagt tgatggttat tgttgttgat
    10261 ttgaataatt tgtagcgaat attttgtaac agggctacta agaattttta taacaagaag
    10321 tgatttcaat cgtactaatt ttcagcgttt gatgacaaaa gtgtatgtcc aaacttaaat
    10381 aatggcgata aaatttaagt catgggtaca tttaatgttt tttgattcaa acttccattt
    10441 tggtcaattt gcttttttta taacatagtt gaatatcaat aaaagattag caagttagtt
    10501 caaaatggct atgtattgtt ttttgaaata cataggtttg aaatctatat taattatgca
    10561 ccaaataatt taattagact ctatcgaaaa tttccaaatt ttgcttacta tctttctaat
    10621 tttactttta tattgtgcac aatgcataaa aacaagcgtt ttcctaggag ttttcattaa
    10681 tttcttttat ttttcactat taccttttca aaaatattca actataatag gtatactatc
    10741 catgatttta aaaatttcca tgttttacat ttaaatgata gatttcaata tatattaggc
    10801 aggagagaga aatatgacat gccaacttaa aatacatcat acattatcga caataccaag
    10861 tcgtaatatt aataatatta tggtgctatt ttcacttaca agtaaactca atattacgat
    10921 taatggtgaa accaaagacg taagtaatta tattatctta attaaccatg gtgatattta
    10981 taatattaac cacggcgaaa atataatcga attaatgata cccgtttttt atttttatca
    11041 acaagatgat gatttcttta atggttattt agatcgtcat ttattacaat caagtaatta
    11101 tattaagtca ttaattgccg atttaatcag tacaccaaca agttcttctt taatggggaa
    11161 aaatataggt caaagtatta tagacacttt acttaaagaa gcttttataa gaatagatca
    11221 tgaatattta ccaaatattg ctttaagcaa tccggtgttc atagactgtg taaactatat
    11281 tcatgacaat attgatgcgc acttgtcttt aaaagatata gcgatgcatt gcaacatctc
    11341 tgaatcatat tgttctaatc tttttgttcg atatttgagt atgaatttta aagattattt
    11401 tacaagtatt aaacttgtta acgccatcaa tttattactt tctacaaaac attctattac
    11461 aactgtttct gaattggccg gttttaacag tcatacaaat tttgcaaatc aatttaagaa
    11521 ttacttgcat tttagtccta aacagttccg ttcactcgtt tccaagatta ctgaaccacc
    11581 acaaatacac ttccaacaag ataacgtatc acaattcact gagttaattt caacaattga
    11641 tttaacagct caactggcta caaatacaac agatattcat atagatgatt ttaatccgaa
    11701 agatcgaagt cagcgtgcca aagtatttgt tcgatttagt aatttcaatg aactatttca
    11761 atttattttc aatgaatatt acgatattaa ctttgagcac ttaccaaaac ctgtcgtttt
    11821 cattgacgat attcacgata ttgagattag ccaaacgaat tacaacctgt taaatagatg
    11881 ttttgaaaag cttttcgaaa aaaatatagg tttagcaatc gctattaaat caacacaaca
    11941 attcgaaacg atgaagcagt taatactaac atttttacaa ggcaatcaag actataagac
    12001 aagcaagaaa ttagttaaat ttatgctcgt attttgctct aattctatga cagcagaaga
    12061 aattcattta tgtcatctta aaattaaaaa caaaaataaa gaaattaaat atagtgtgac
    12121 agtcgatggc tttttagaaa cttattcaac cgtggaacaa gtttatgatg tcatgcagcg
    12181 tcttaaattc cattattact ttattgatat tgaaaattca aaaactgcaa cacatcttat
    12241 cactaaaaac caacactatc atcaaactga cactcatttt gaacagtata agaaatttat
    12301 tttagattca ggtatatcat caacacaatt tgtatataat aacctgtctg taagcggatt
    12361 taaatatact aatgatggta agaatccaat tcaattatct gacatagtgt atcacttaat
    12421 cgcattatta cgatatggcg gtggcattag ctatcaatta ttagatgacc attcaaatta
    12481 tatttccttg tacaacaaat atggtagccc cctaccatta atgcatttat ataaaatgtt
    12541 tagacctttt gttaatgaag atattgaaat tacaaataat tatgtattga gtcgtaaaga
    12601 taataactac catttcttat tattcaataa aattaatgat cgatatatgt cagacgtaaa
    12661 acaagatttc attttccata atgaattacc tcaagactct ttgatgatta ttaaaacatt
    12721 gaatcatgaa catggttcaa ttcaacattt gcttccaata agcgatcaac ttgtttatat
    12781 agaaaaagaa attttagatg aattagacaa aacgaattac cctaaaacgg agcttgcagt
    12841 tcaagaagaa actggtcgta catttgaact caagttaaat cacgacgagg ttaaatatat
    12901 ttgctttaaa ccaagctaaa tactaacagt cctcttgtgt ttagtttctt acgttaaagg
    12961 ctatttatat cataaggaga tgatatgtaa tgagtaactc acaagcaatt caagcaattg
    13021 aaaacgtgtt agcaacgtca aaagttggtg tattatcaac tgcatataat aacaaaccta
    13081 atagtagata tatggtcttt tataatgatg gtcttacttt atatactaaa acgaatatcc
    13141 attctgctaa ggtcaaagaa attaaagata atccagcagc atatgttttg ttaggctata
    13201 acgacacaac taatcgtagt ttcgttgaaa tggaagccac gatagaagta gttactgatc
    13261 aaaaagtaat cgattggtta tgggagactc aagataaaag ctttttcagt tcaaaagaag
    13321 acccagaact ttgtgtttta aaggtgacac cacaatctgt taaattgatg aatgacaaat
    13381 cattagatac acctatcaaa atcgatttat aacacaaagt atatatagga aataactttt
    13441 atgaattcta gatataacaa tgttaaatac ttaaaataac tcgctataat taaagtgttt
    13501 aatatgttta caatccaatt tgcaatgtat tgtttttaga cacactttat tcacatttaa
    13561 cgtgattagg gtgtgtcttt ttaaattaat tttatgtaat ataaatactg catttgcaaa
    13621 ctgttgcact tttaggtata acagaattaa ctacatttaa ggagattgat gaaccatgaa
    13681 aaagaaaaaa ggttttggtc ttggtattag tttaatcgcc atcatgttaa ttgtatgtat
    13741 tgtattagta atcatgatga tgactggcgg aaagaaagat acatactatg gaattatgaa
    13801 agataatact actattgaaa aaatgattag tgaaaaagat gaaagtattg aaaaaaatgt
    13861 taaattacct tcagattcag atgttaaagt taaaaaaggt gattttgtaa ttgtttataa
    13921 attagcagat tcagataaaa ttgttaaagt taaaaaagtt gaccatgatg acgtccctca
    13981 cggtttaatg atgaaaattc atgacatggg caaaatgcac atgaaacact aattaagctt
    14041 taattttgag taatatagaa gatattaaaa gcagcagtaa gatgttttct aattgaaaat
    14101 tatcttactg ctgcttttta agaattactg tgtcagtcta tttttggttc gcctgcgctt
    14161 ttgcgcatgc ataaaagccc ctaacaacct gaggtcactg cgcttcggtt cgcctgcgct
    14221 tttgcgcatg cataaaagcc cctaacaacc tgaggtcact gcgcttcggt tcgcctgcgc
    14281 ttttgcgcat gcataaaagc ccctaacaac caaaggttgt aaggggcttt aaatgtcaga
    14341 tagtattttt taaattgatt ttaaatattc ttgacctttt gctttgtcat atacgttttc
    14401 ccatttagct ataacgattg ttgataatgc gttaccaata acgtttacac atgtacgtac
    14461 catatctaag atacggtcaa caccaataat taatgctaaa ccttgtgccg gtaagcccat
    14521 ggcacctaat gttgttaata aaacaacgat tgaagttcct ggtactgccg ccataccttt
    14581 agacgtaatc attaatgtta acatcaacac aatttgctct gataatgtta agtgcattcc
    14641 atacatctgt gcaacgaata atgctgcaat agattgataa agtgctgatc cgtctaagtt
    14701 aaacgtataa ccaattggta taacaaaaga agtaatttct tttggagaac cgaagttttc
    14761 cattttcttc atcattacag gaagtacagc ttccgaactt gatgtagaaa atgctaaaag
    14821 caattcactt ttcaagattc taataatatt catgatatta ataccacaca tccatgcaac
    14881 tagtcctagt atagcgaata caaagaacac cattgcaaag acaacaacta atactaattt
    14941 taatagtggt aataatgcgg atgcaccaaa tgtaataatt gtagtacaaa tgaatgcaaa
    15001 cactccaagt ggtgctaatt ttaaaatttt attaatcatc cagaacacag cttcaagcga
    15061 tccgcttaaa aattctttaa ctggttctgc ttttttacct acagcagcta atcctaatcc
    15121 aaagaatact gcgaagaaga taataggtaa taattcaccc ttatttaaag cttcaaaaaa
    15181 gtttgtcgga ataatatgta caatggtatc aataaaatga tttccatatg tagattgttc
    15241 tgctgcatgt gcagttgatt gatatttaga aatatcacct ttaggtaatt ttgttgggtc
    15301 taatccagca cctggtttaa ataggttacc gaagataatc cctaaaccta ttgcgattgt
    15361 tgtaataatt tcaaagtata aaattgtttt ccagccataa cgccctacag ttttcgattc
    15421 cccaacgttc gaaatagaaa gcgctagtga gcaaaataca actggtataa cgatcatttt
    15481 aattaaattt aaaaatacgt caccaaatgg tttaatgtaa tttgctacgt tttcttgtcc
    15541 atataataaa agtcctacga caacacctaa cactaatgca ataacaactt gcataggtag
    15601 gctaattttt ctcttgaata gagccatagt acaaactccc cttcatctga catcac
//
LOCUS       DANBWH010000043        15553 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.045, whole genome shotgun sequence.
ACCESSION   DANBWH010000043 DANBWH010000000
VERSION     DANBWH010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15553
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.045"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            131..1990
                     /locus_tag="PPY92_000949"
     CDS             131..1990
                     /locus_tag="PPY92_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HDJ2624967.1"
                     /translation="MPKNKILIYLLSTTLVLPTLVSPTAYADTPQKDTTAKTTSHDSK
                     KSNDDETSKDTTSKDTDKADNNNTSNQDNNDKKFKTIDDSTSDSNNIIDFIYKNLPQT
                     NINQLLTKNKYDDNYSLTTLIQNLFNLNSDISDYEQPRNGEKSTNDSNKNSDNSIKND
                     TDTQSSKQDKADNQKAPKSNNTKPSTSNKQPNSPKPTQPNQSNSQPASDDKANQKSSS
                     KDNQSMSDSALDSILDQYSEDAKKTQKDYASQSKKDKNEKSNTKNPQLPTQDELKHKS
                     KPAQSFNNDVNQKDTRATSLFETDPSISNNDDSGQFNVVDSKDTRQFVKSIAKDAHRI
                     GQDNDIYASVMIAQAILESDSGRSALAKSPNHNLFGIKGAFEGNSVPFNTLEADGNKL
                     YSINAGFRKYPSTKESLKDYSDLIKNGIDGNRTIYKPTWKSEADSYKDATSHLSKTYA
                     TDPNYAKKLNSIIKHYQLTQFDDERMPDLDKYERSIKDYDDSSDEFKPFREVSDSMPY
                     PHGQCTWYVYNRMKQFGTSISGDLGDAHNWNNRAQYRDYQVSHTPKRHAAVVFEAGQF
                     GADQHYGHVAFVEKVNSDGSIVISESNVKGLGIISHRTINAAAAEELSYITGK"
     gene            complement(2255..2815)
                     /locus_tag="PPY92_000950"
     CDS             complement(2255..2815)
                     /locus_tag="PPY92_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDJ2624968.1"
                     /translation="MSRKTALLVLDMQEGIASSVPRIKNIIKANQRAIEAARQHRIPV
                     IFIRLVLDKHFNDVSSSNKVFSTIKAQGYAITEADASTRILEDLAPLEDEPIISKRRF
                     SAFTGSYLEVYLRANDINHLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDD
                     KHDFIIEQILSRSCDIESVESWKSSL"
     gene            complement(2984..4891)
                     /gene="sasF"
                     /locus_tag="PPY92_000951"
     CDS             complement(2984..4891)
                     /gene="sasF"
                     /locus_tag="PPY92_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasF"
                     /protein_id="HDJ2624969.1"
                     /translation="MAKYRGKPFQLYVKLSCSTMMASSIILTNILPYDAQAASEKDTE
                     ISKEILSKQDLLDKVDKAIRQIEQLKQLSASSKAHYKAQLNEAKTASQIDEIIKRANE
                     LDSKENKSSHTEMNGQSDIDSKLDQLLKDLNEVSSNVDRGQQSGEDDLNAMKNDMSQT
                     ATTKYGEKDDKNDEAMVNKALEDLDHLNQQIHKSKDALKDASKDPAVSTTDSNHEVAK
                     TPNNDGSGHVVLNKFLSNEENQSHSNQLTDKLQGSDKINHAMIEKLAKSNASTQHYTY
                     HKLNTLQSLDQRIANTQLPKNQKSDLMSEVNKTKERIKSQRNIILEELARTDDKKYAT
                     QSILESIFNKDEADKILKDIRVDGKTDQQIADQITRHIDQLSLTTSDDLLTSLIDQSQ
                     DKSLLISQILQTKLGKAEADKLAKDWTNKGLSNRQIVDQLKKHFASTGDTSSDDILKA
                     ILNNAKDKKQAIETILATRIERQKAKLLADLITKIETDQNKIFNLVKSALNGKADDLL
                     NLQKRLNQTKKDIDYILSPIVNRPSLLDRLNKNGKTTDLNKLANLMNQGSNLLDSIPD
                     IPTPKPEKTLTLGKGNGLLSGLLNADGNVSLPKAGETIKEHWLPISVIVGAMGVLMIW
                     LSRRNKLKNKA"
     gene            complement(5132..6490)
                     /gene="gtfB"
                     /locus_tag="PPY92_000952"
     CDS             complement(5132..6490)
                     /gene="gtfB"
                     /locus_tag="PPY92_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HDJ2624970.1"
                     /translation="MIKLFDYFNDHSRKLYESFKASKLEEDLTIVLNDNGFLPDDVIS
                     PYQFFADNHNTENMKPRFFNQVTVPAFWEIKGNNNSATINDMGRLRGKIFYQSGERPR
                     IVSRVEWFDDQQRVRFVDYYSKNGIKFAQTVYDLNRKAILKKYMTAEGKEVIYENFVT
                     SDVILDWQGKSYFFPSKLAFVLFFIKQLEITEHHFVINSLALPFSVLYNLPSKGSDVL
                     VWQEHCNGNVPGNMQLMCKGDMKRHCNIIIPDKNEYETMLNIADAKVQSRILQGGYLY
                     NYRSRNRYTKEIVILTNSDQLRNIKVLVETLPDFNFHIAAITEMSDKLMQLDQYANVH
                     LYPSINIDRVNELYQLCDIYLDINEGNEILNAVEQAFDYELLILGYRQTAHHAKVTLS
                     EHLFEHNDEITMESKDQLIQMLESLKDQQQFRDALLAQKAHAHEISREQFEQVFKQAL
                     ES"
     gene            complement(6483..7991)
                     /gene="gtfA"
                     /locus_tag="PPY92_000953"
     CDS             complement(6483..7991)
                     /gene="gtfA"
                     /locus_tag="PPY92_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HDJ2624971.1"
                     /translation="MTIYNINFGIGWASSGVEYAQAYRAKLLRRTNQDTKFVFLDFIQ
                     SENIQTLTQNIGFKDQEVIWLYQYFSDISIAPTTYTLDDIERELGHEISFRERNGKIV
                     RLYFNGKSSFVTCYLQNEQKDVVDRAEFVINSMLVRKDFYSYTRIFSEYYAPADNKAK
                     LYMRQFYNEDGSIAYNEYIDGDSSIYVFEDAVLYNKTEFIAYFLQRLNLTRDDIVILD
                     RASDIGQAVLQHKGDSKVGVVIHADHYSNNMMSEQHILWNNYYEYQFSKAKYIDFFIT
                     ATDIQNHMVCRQFEQYQGYRPRVYTIPVGSIDALSYPKLSRKPYAMISASRLANEKHI
                     DWLVKAVIVAKRQVPELTFDIYGEGSEKTRLRKIIDTHRAQDYIRLLGHVKLDEIYND
                     YELFLSASTSEGFGLTLMEAVGSGLGMIGLNVNYGNPTFIRDGENGYLVPFDTDEDSV
                     DDVIAKLAHAIVMYFNNGPQAPHDISYEVAQQFMTQDIILKWETLVQEVLHD"
     gene            complement(8009..10399)
                     /gene="secA2"
                     /locus_tag="PPY92_000954"
     CDS             complement(8009..10399)
                     /gene="secA2"
                     /locus_tag="PPY92_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HDJ2624972.1"
                     /translation="MKHKLDVTINELRLKSIRKIVKRINTWSDEVKSYSDDALKQKTI
                     EFKERLASGVDTLDTLLPEAYAVAREASWRVLGMYPKEVQLIGAIVLHEGNIAEMQTG
                     EGKTLTATMPLYLNALSGKGTYLITTNDYLAKRDFEEMQPLYEWLGLTASLGFVDIVD
                     YEYQKGEKRNIYEHDIIYTTNGRLGFDYLIDNLADSAEGKFLPQLNYGIIDEVDSIIL
                     DAAQTPLVISGAPRLQSNLFHIVKEFVDTLIEDVHFKMKKTKKEIWLLNQGIEAAQSY
                     FNVEDLYSEQAMVLVRNINLALRAQYLFESNVDYFVYNGDIVLIDRITGRMLPGTKLQ
                     AGLHQAIEAKEGMEVSTDKSVMATITFQNLFKLFESFSGMTATGKLGESEFFDLYSKI
                     VVQAPTDKAIQRIDEPDKVFRSVDEKNIAMIHDIVELHETGRPVLLITRTAEAAEYFS
                     KVLFQMDIPNNLLIAQNVAKEAQMIAEAGQIGSMTVATSMAGRGTDIKLGEGVEALGG
                     LAVIIHEHMENSRVDRQLRGRSGRQGDPGSSCIYISLDDYLVKRWSDSNLAENNQLYS
                     LDAQRLSQSNLFNRKVKQIVVKAQRISEEQGVKAREMANEFEKSISIQRDLVYEERNR
                     VLEIDDAENRDFKALAKDVFEMFVNEEKVLTKSRVVEYIYQNLSFQFNKDVACVNFKD
                     KQAVVTFLLEQFEKQLALNRKNMQSAYYYNIFVQKVFLKAIDSCWLEQVDYLQQLKAS
                     VNQRQNGQRNAIFEYHRVALDSFEVMTRNIKKRMVKNICQSMITFDKEGMPVIHFP"
     gene            complement(10389..11348)
                     /gene="asp3"
                     /locus_tag="PPY92_000955"
     CDS             complement(10389..11348)
                     /gene="asp3"
                     /locus_tag="PPY92_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HDJ2624973.1"
                     /translation="MLKNKTFKVTWNYISQTTFMYGSKVSFSNGEVTFINPLMPSGLP
                     IHEWLMLKQFSKYKSAPSLPILRRGQHYKLHFDFDATPAGSVYFIIIFYNKNGTKLST
                     EIVKSNSITIQYPDEAYAYKIKMMNAASTSLIFRCLTITEMTHQDDLEYKSMRVTKID
                     DNQYGNDRINVIIAEPSDTYPTISNDFLKLFGHVWLVERWMDDDIKENIKQLKDDLQS
                     QDTLTAINLISYGSKSNVFATYVAQHLDCKVYRTSHEDDDLKEWLTEHVPGNNELKDT
                     NVEFYFKEEQDNHLNYMSRLMNPVRFLDYLDVSKLNGGEVNET"
     gene            complement(11326..12894)
                     /gene="asp2"
                     /locus_tag="PPY92_000956"
     CDS             complement(11326..12894)
                     /gene="asp2"
                     /locus_tag="PPY92_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HDJ2624974.1"
                     /translation="MPRKFRVLQIGGDDLEPIFQHKKGVSWDYFDIGLFEFDSGYVEA
                     IEAIVEAEGRFDFIYIQAPYSETLTNLLQMISEPYNTYVDESFWSVEYEQDENVQKYV
                     VQPLHYRNIEERNNKLEAVSFSGQYGDKVSPKLALVHPNFKVDVVYQGNSELTLSGEF
                     GKEFKPIASWQNNLVYDKDKVIQIWPEFDIDGAVELQYTFRLIQTGADGALIEQIILT
                     DDMLDSPLEIPAKPFDAYISVTVKARGNGTVHLGPIHKRWSRLDMGQFLLGGSRFVDS
                     QRQEFIYYFHPGDMKPPLNVYFSGYRTAEGFEGYYMMKRMNAPFLLIGDPRVEGGSFY
                     IGSSEYEQGIINVIDETLEKLNFKSHELILSGLSMGSFGALYYGAQLNPQAIIVGKPL
                     VNIGTIAEHMRLLRPEEFGTALDVLVSNEGDTSQASIKALNQKFWQTFQKKSLSQTVF
                     AIAYMQHDDYDPHAFQELLPVLTAHQARVMNRSIPGRHNDDSPTIASWFVNFYNIILE
                     DKFGRVQHAEKQNI"
     gene            complement(12884..14437)
                     /gene="asp1"
                     /locus_tag="PPY92_000957"
     CDS             complement(12884..14437)
                     /gene="asp1"
                     /locus_tag="PPY92_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HDJ2624975.1"
                     /translation="MKYFIPAWYDDQRWWQDTTVPYYQLQNKTEFDDMISLMGMHLEN
                     DLDYQLIVLNHAPNLRTFLHRYDLYETKYSSVFDEIQGFSHHAPQAINYHHLKWPDDV
                     EFVYTPYLLKCVTSEQTYTNIYFSQEGYSIWFEEFERDQLQRRYIFDDRGYLSAIRYF
                     DDQGEASYQQYLTINGDCVLHEDLKNGRVTVSKRYQHHYQQTEYNNMAQLIEEKFQAM
                     IAQQIHEDDHVIVASDARHNRQIANHIPAKLLSYSFFKNRNETVSDEEYQSIVKNAHL
                     IVDSVQLERDLISHQEKYQRENTMIRITPFETRQSPNISSQLMETFIGVWIDGMSDAD
                     LQQMMQRLVDYIAQEDYYRLILLSRHQNDIPMWLRECITSVNEEYQAKQNADVNVSAL
                     MTPEDQDDIIAVKTIHAEHDVVEALRTLRLVIDMSKEPDLYLQISAISAGIPQINGQQ
                     TDYVSDYDNGRIINTVDELDDALNYYLFYLKNWNYAYAYSLKLIDAYASKNIINQLDE
                     LIEGENDAT"
     gene            complement(14448..>15553)
                     /gene="secY2"
                     /locus_tag="PPY92_000958"
     CDS             complement(14448..>15553)
                     /gene="secY2"
                     /locus_tag="PPY92_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HDJ2624976.1"
                     /translation="SYNDMQVKHESFFKIAISNMGGDVNTLNIFTLGLVPWLTSMIIL
                     MLISYRNMDKYMKQTSLEKHYKERILTLILSVIQSYFVIHEYVSKERVHQDNIYLTIL
                     ILVTGTMLLVWLADKNSRYGIAGPMPIVMVSIIKSMMHQKMEYIDASHIVIALLIILV
                     IITLFILLFIELVEVRIPYIDLMNVSATNMKSYLSWKVNPAGSITLMMSISAFVFLKS
                     GIHFILSMFNKSISDDMPMLTFDSPVGISVYLVIQMLLGYFLSRFLINTKQKSKDFLK
                     SGNYFSGVKPGKDTERYLNYQARRVCWFGSALVTVIIGIPLYFTLFVPHLSTEIYFSV
                     QLIVLVYISINIAETIRTYLYFDKYKPFLNQYW"
BASE COUNT         5009 a         2988 c         2170 g         5386 t
ORIGIN      
        1 ataatcaatt tactggaaga tatttagtcg attgatacct atcaactatt ttcagcatac
       61 gataaattat aacaaatcat agtttattat cacacttaat tattatattt ttcaagggag
      121 aatacgaaat atgcctaaaa ataaaatttt aatttatttg ctatcaacta cgctcgtatt
      181 acctacttta gtttcaccta ccgcttatgc tgatacacct caaaaagata ctacagctaa
      241 gacaacatct catgattcaa aaaaatctaa tgacgatgaa acttctaagg atactacaag
      301 taaagatact gataaagcag acaacaataa tacaagtaac caagacaata acgacaaaaa
      361 atttaaaact atagacgaca gcacttcaga ctctaacaat atcattgatt ttatttataa
      421 gaatttacca caaaccaata taaaccaatt gctaaccaaa aataaatacg atgataatta
      481 ctcattaaca actttaatcc aaaacttatt caatttaaat tcggatattt ctgattacga
      541 acaacctcgt aatggcgaaa agtcaacaaa tgattcgaat aaaaacagtg acaatagcat
      601 caaaaatgac actgatacgc aatcatctaa acaagataaa gcagacaatc aaaaagcacc
      661 taaatcaaac aatacaaaac caagtacatc taataagcaa ccaaattcgc caaagccaac
      721 acaacctaat caatcaaata gtcaaccagc aagtgacgat aaagcaaatc aaaaatcttc
      781 atcgaaagat aatcaatcaa tgtcagattc ggctttagac tctattttgg atcaatacag
      841 tgaagatgca aagaaaacac aaaaagatta tgcatctcaa tctaaaaaag acaaaaatga
      901 aaaatctaat acaaagaatc cacagttacc aacacaagat gaattgaaac ataaatctaa
      961 acctgctcaa tcattcaata acgatgttaa tcaaaaggat acacgtgcaa catcattatt
     1021 cgaaacagat cctagtatat ctaacaatga tgatagcgga caatttaacg ttgttgactc
     1081 aaaagataca cgtcaatttg tcaaatcaat tgctaaagat gcacatcgca ttggtcaaga
     1141 taacgatatt tatgcgtctg tcatgattgc ccaagcaatc ttagaatctg actcaggtcg
     1201 tagtgcttta gctaagtcac caaaccataa tttattcggt atcaaaggtg cttttgaagg
     1261 gaattctgtt ccttttaaca cattagaagc tgatggtaat aaattgtata gtattaatgc
     1321 tggattccga aaatatccaa gcacgaaaga atcactaaaa gattactctg accttattaa
     1381 aaatggtatt gatggcaatc gaacaattta taaaccaaca tggaaatcgg aagccgattc
     1441 ttataaagat gcaacatcac acttatctaa aacatatgct acagatccaa actatgctaa
     1501 gaaattaaac agtattatta aacactatca attaactcag tttgacgatg aacgcatgcc
     1561 agatttagat aaatatgaac gttctatcaa ggattatgat gattcatcag atgaattcaa
     1621 acctttccgc gaggtatctg atagtatgcc atatccacat ggccaatgta cttggtacgt
     1681 atataaccgt atgaaacaat ttggtacatc tatctcaggt gatttaggtg atgcacataa
     1741 ttggaataat cgagctcaat accgtgatta tcaagtaagt catacaccaa aacgtcatgc
     1801 tgctgttgta tttgaggctg gacaatttgg tgcagatcaa cattacggtc atgtagcatt
     1861 tgttgaaaaa gttaacagtg atggttctat cgttatttca gaatccaatg ttaaaggatt
     1921 aggtatcatt tctcatagaa ctatcaatgc agctgccgct gaagaattat catatattac
     1981 aggtaaataa gtattattaa acccgcaaaa tttataagta taaacaagga gttcggactt
     2041 aaacatattt ctgttcataa gtccgatttc ttattcaatt aaacccgagg tattcagttc
     2101 gaacgcctcg ggtcatttta tataaatata ttattttatg ttcaaatgtt cctcatcata
     2161 tccgtttcaa ttgtcatctc acacatttta taaatatgag caaatgtact tattttcaaa
     2221 cattactgcc tagctttaat tgacgttata ttaactataa actacttttc catgactcta
     2281 cggattcaat gtcacatgag cgtgataaaa tttgttcaat aataaagtca tgtttatcat
     2341 ctgatctatc accaacagca tcttctaaaa cagtaatata atagtcttta tctacacttt
     2401 ctaatgccgt gctcaataca gctccactcg tagagacacc cgttaatact aaatgattaa
     2461 tatcatttgc acgtaaataa acttccaagt aactacctgt aaatgcgcta aagcgtcgct
     2521 tagaaataat cggctcatct tctagtggtg ctaaatcttc aagtattcgt gtagatgcat
     2581 ctgcttcagt aatcgcatat ccttgagctt taattgttga aaacacttta ttactcgagg
     2641 agacatcatt aaaatgctta tctaacacta aacgtatgaa aatgactggt attcgatgtt
     2701 gtcttgctgc ttcaattgct ctctgattcg ctttaataat attttttatt ctaggtacac
     2761 tactcgctat accttcttgc atatccaaaa ctaatagcgc cgtttttcga gacatcttca
     2821 ttctccttta cttctgtagt tctaagtcgt taaattcatt ataacgttaa aatgatggac
     2881 aatctattca ttgcattttg catatacttc acaataattt aagggggaaa taagacgtct
     2941 tatatactta aaaaaatata tagatgctct tcccccaata taattatgct ttatttttca
     3001 acttattgcg tcgtgataac caaatcatta gtacacccat tgcaccaaca attacagata
     3061 tcggcaacca atgttctttt atcgtttccc ccgctttagg caaagataca ttaccatcag
     3121 catttaataa tccacttaac aatccattac ctttaccaag tgttaacgtc ttttctggct
     3181 ttggtgtggg tatatctgga atactgtcta ataaatttga tccttgattc attaaatttg
     3241 ctaacttatt taaatccgtt gttttcccat ttttattcaa tcgatctagt aaacttggac
     3301 gatttactat tggtgataaa atatagtcta tatctttttt cgtttgattg agtctctttt
     3361 gtaaattcaa taaatcatcc gctttaccat tcaatgccga tttaactaaa ttaaaaattt
     3421 tattttgatc tgtttctatt ttagtaatta aatctgccag taattttgcc ttttgtcttt
     3481 ctatacgtgt tgctaaaatc gtttcaattg cttgcttttt atctttggca ttattcaaaa
     3541 ttgcttttaa tatatcatct gaagacgtgt cgccagttga tgcaaaatgt ttcttcaatt
     3601 ggtcaacgat ttggcgattt gataatcctt tattcgtcca atctttagcc aatttatctg
     3661 cttcagcttt tcctaatttc gtttgtaaga tttgagaaat caatagcgac ttatcttgtg
     3721 attgatcaat caatgacgtt aataaatcat cactcgttgt cagagatagt tgatcaatat
     3781 gacgagtaat ttgatctgca atttgttgat ctgttttacc atcaacacgt atatctttta
     3841 gaattttatc tgcctcgtct ttattaaata tactttctaa aatgctttgt gtagcatact
     3901 ttttatcatc agtacgtgca agttcttcca aaataatatt tcgttgactt tttatacgct
     3961 ctttcgtctt atttacttcg ctcattaagt ctgatttttg atttttagga agttgcgtat
     4021 ttgcaatacg ttgatctaaa gattgtaacg tattcagttt atgatatgtg taatgttgcg
     4081 ttgaggcatt acttttagcc aatttttcaa tcatagcatg attaatttta tcgcttcctt
     4141 gtaatttatc agtgagttga ttactatggc tttgattctc ttcatttgaa agaaatttat
     4201 ttaacacaac atgtccagaa ccatcattat ttggcgtttt agctacttca tgattactat
     4261 ctgttgtaga cactgccgga tctttcgatg catctttcaa tgcatctttc gatttgtgta
     4321 tttgctgatt caaatggtct aggtcttcta acgccttatt taccattgct tcatcatttt
     4381 tatcatcttt ttctccatat tttgttgtag ccgtttgtga catatcattt ttcattgcat
     4441 taagatcgtc ctcgccactt tgttgacccc tatcaacatt tgaagaaacc tcatttaaat
     4501 ctttaagcaa ttgatctaat ttactgtcta tatcactttg accgttcatt tcagtgtgag
     4561 aacttttatt ttctttgcta tccaactcat tagctcgttt tatgatttca tctatttgcg
     4621 atgctgtttt cgcttcattt agttgtgctt tataatgtgc tttagatgaa gccgataact
     4681 gttttaattg ctcaatttga cgaattgctt tgtcaacttt gtctaataaa tcttgcttag
     4741 ataatatctc ttttgaaatt tcagtatcct tttcagatgc agcttgggca tcgtacggca
     4801 agatattcgt taaaatgata cttgacgcca tcattgtcga acacgataac tttacatata
     4861 attgaaacgg tttccctcga tatttagcca tcaacatact cctttctcac ttacttcctt
     4921 caaagaatta catactatta tatacctgtt tacaagaaat ttacacttat ctatctagtt
     4981 attgttgtta gtaattatca acttattact tagcttatat ttaagtaaac aaaaaagcat
     5041 gacgtaatat catattgtcc atgtcgctaa catcatatta cgtcaaatct tttaaattaa
     5101 atgatgcttt attttagact gctttttctt tttagctttc gagcgcctgt ttaaaaactt
     5161 gctcgaattg ttcacgcgag atttcgtgtg catgtgcttt ttgtgctaat aaagcatctc
     5221 gaaactgttg ttgatctttc aaactttcta acatttgtat taattggtct ttactttcca
     5281 ttgttatctc atcattatgc tcaaataagt gctctgataa tgttacttta gcatggtgtg
     5341 cggtttgacg ataacctaaa atcaacaact catagtcaaa cgcttgttcc accgcattta
     5401 aaatttcatt accctcattg atatcaagat aaatatcaca taactggtat agttcattta
     5461 ccctgtcaat attaatagat gggtataagt gcacattagc atattgatca agttgcatta
     5521 gcttatcaga catctctgta atagcagcga tgtgaaaatt aaaatctggt aaagtttcaa
     5581 ccaatacctt gatgttacga agttgatccg agttagttaa tattacaatt tctttagtat
     5641 atctattacg actacgatag ttatatagat atccgccttg taaaatacga gattgaacct
     5701 ttgcgtctgc tatattgagc atcgtttcat attcgttttt atctggaata ataatattac
     5761 aatgtcgttt catatcacct ttacacatca attgcatatt tcccgggaca ttaccattac
     5821 agtgttcttg ccataccaaa acatcactac cttttgatgg caaattatat aacactgaaa
     5881 atggtagggc tagtgagtta ataacgaaat gatgttccgt aatttcaagt tgcttgataa
     5941 aaaataatac gaatgcgagc tttgaaggga aaaagtaaga cttcccttgc caatccaata
     6001 tgacatcaga tgttacaaaa ttttcataaa tcacttcttt accttctgct gtcatatatt
     6061 tcttcaagat cgctttacga tttaaatcgt aaacagtttg tgcaaattta ataccattct
     6121 tagaataata atcgacaaat cggacacgtt gttggtcatc aaaccattcg acacgactaa
     6181 caattctagg gcgctctcca ctttgataaa atattttgcc tcgtagacgt cccatatcat
     6241 taattgtagc cgaattgttg ttacctttaa tttcccaaaa agctggtaca gtaacctgat
     6301 taaaaaatcg tggtttcata ttttctgtat tatgattatc tgcaaaaaat tgatacggtg
     6361 atataacatc gtccggtaaa aagccattgt cattgagtac aattgttaaa tcttcttcca
     6421 acttactggc tttaaaagac tcatataact ttcgtgaatg atcgttaaag taatcaaata
     6481 atttaatcat gtagcacctc ttgaactaat gtttcccatt ttaaaataat atcttgagtc
     6541 ataaattgct gtgccacttc ataagagatg tcatgtggtg cctggggacc attgttaaaa
     6601 tacattacaa tggcatgagc tagttttgcg ataacatcat ccacactatc ttcgtcggta
     6661 tcaaaaggta ccaagtagcc attttcccca tctcgaataa aggttgggtt accataattc
     6721 acatttaatc caatcatacc tagtcctgag cctaccgctt ccattagtgt taacccaaaa
     6781 ccttcgctag ttgatgcaga aagaaataac tcataatcat tataaatttc atcaagttta
     6841 acatgcccta gtaaacgaat ataatcttgt gcgcggtgtg tatcaataat tttacgcagt
     6901 cgcgtcttct cgctaccttc tccataaata tcaaatgtta attctggcac ttgtcgttta
     6961 gccacgataa ccgctttgac aagccaatca atatgtttct catttgctaa acgagatgca
     7021 ctaatcatcg catatggctt tcttgataat ttaggatatg ataacgcatc aatgcttccc
     7081 accggaatag tatagacacg tggacgataa ccttgatatt gctcaaattg tcgacaaacc
     7141 atatgatttt gaatatctgt tgctgtaata aagaaatcaa tgtatttagc ttttgaaaat
     7201 tgatattcat aataattgtt ccatagtata tgctgctcgc tcatcatatt attactataa
     7261 tgatcagcat gaatcacaac accaacttta ctatcacctt tatgctgcaa aacagcctga
     7321 ccaatatcag aagcgcggtc taatatgaca atatcgtctc gggttaaatt caatcgttgt
     7381 aaaaagtatg caataaattc cgttttgtta tacaacaccg catcttcaaa cacatatata
     7441 gagctgtctc catcaatata ttcgttataa gcgatggaac catcttcatt atagaattgt
     7501 cgcatatata atttcgcttt attatcagct ggtgcataat actcagaaaa tatacgcgta
     7561 taactataaa aatctttacg tactaacata ctattaatta caaattctgc acgatccaca
     7621 acatcttttt gttcattttg tagataacat gttacaaatg atgatttccc attaaaatat
     7681 agacggacta tcttaccatt tctttctcta aaactaattt catgaccaag ctcacgttca
     7741 atgtcatcta acgtgtacgt tgttggtgct atagaaatat cactaaaata ctgatacaac
     7801 caaataactt cttgatcttt aaacccaatg ttttgcgtta atgtctgtat gttctctgac
     7861 tgtataaaat ctaaaaacac aaatttagtg tcttgatttg tacgtctcaa taatttagca
     7921 cggtaagctt gtgcatattc aacaccgcta ctcgcccagc ctataccaaa gtttatatta
     7981 tatattgtca tgcgctaccc cttttcattt atggaaaatg tataactggc ataccctctt
     8041 tatcaaatgt aatcatgctt tgacaaatat ttttcaccat tctttttttg atatttcgtg
     8101 tcataacttc aaatgaatct aatgcaactc tatggtattc aaaaatagcg ttacgttgcc
     8161 cattttgtcg ttgattaacg cttgctttta attgttgtaa atagtcgact tgttctaacc
     8221 aacatgaatc aattgctttc aaaaagactt tttgaacgaa aatattataa taatatgcac
     8281 tttgcatgtt tttacgattc aaagctaatt gcttttcaaa ttgctctaat aaaaatgtca
     8341 ctactgcttg cttatcttta aaattaacac aagccacatc tttattaaat tggaaactta
     8401 aattttgata aatatactcg acaacacgcg attttgttag caccttttcc tcatttacaa
     8461 acatttcaaa tacatcttta gctaacgctt taaaatctcg attctcagca tcatctattt
     8521 ctaaaactcg attgcgttcc tcgtatacaa gatctcgctg tatactaatg cttttttcaa
     8581 attcattagc catttcacga gctttaaccc cttgttcttc cgagatacgc tgcgctttaa
     8641 ctacaatttg cttaactttg cgattaaaca aattactttg cgataatcgt tgtgcatcta
     8701 atgaatataa ttgattattt tccgctaaat tactatcgct ccatcgctta actaaataat
     8761 catctagtga aatatatata caagatgatc ccggatcccc ttgtctacca gaacgaccac
     8821 gtaattgcct gtctacacgg ctattttcca tatgttcatg aataataaca gctaatccac
     8881 ctaatgcttc gacaccttca ccaagtttaa tatctgtgcc tcgacctgcc atactagtcg
     8941 caacagtcat ggaaccaatt tgccctgctt cagctatcat ctgcgcttct tttgcaacat
     9001 tttgcgcaat gagtaaatta ttaggaatat ccatttggaa taatactttc gaaaagtatt
     9061 cagccgcttc agcagttctc gttatgagta aaaccggtcg ccccgtttca tgaagttcaa
     9121 ctatatcatg aatcatcgcg atgtttttct catcaactga acgaaacact ttatctggtt
     9181 catcgatacg ttgaatcgct ttatcagttg gtgcttgtac gactattttt gaatacaaat
     9241 caaagaactc tgattcgcct aattttcctg tagctgtcat acctgaaaat gattcaaaaa
     9301 gtttaaataa attctggaag gtaattgttg ccataacact tttatctgtt gaaacctcca
     9361 taccttcttt cgcttcaata gcttggtgaa gtccagcttg caacttagtt cccggtaaca
     9421 tacgacctgt aatacggtca attaaaacaa tatcaccatt atatacaaag taatcgacat
     9481 tagattcaaa caaatattgt gcgcgcagtg ctaaattaat attacgcact aggaccatcg
     9541 cttgttcgct atataaatct tcaacattaa agtatgattg tgccgcttca ataccttgat
     9601 ttaacagcca tatttctttt ttggtcttct tcattttaaa atgcacgtct tcaatcaatg
     9661 tatctacaaa ctctttcaca atatgaaata gatttgattg taatcttggt gcacccgaaa
     9721 taactaatgg tgtttgagca gcatctaaaa tgattgaatc cacttcatca ataataccgt
     9781 aatttaattg tggtaaaaat ttcccttccg cactatcagc caaattatca attaaataat
     9841 caaaaccgag acgtccatta gttgtatata taatatcatg ttcatatata ttacgttttt
     9901 cccctttttg atactcataa tccacaatat caacaaaacc taatgaagca gttaaaccta
     9961 accattcata taatggttgc atctcttcaa aatctcgctt cgctaaataa tcattcgttg
    10021 taattaaata tgttcctttt cccgaaagag catttaaata taaaggcatc gttgccgtta
    10081 atgttttacc ttcgcctgtt tgcatctccg caatgttacc ttcatgcaat acaatcgctc
    10141 cgattaactg aacttcttta ggatacatac ctaatactct ccagctcgct tcacgtgcca
    10201 ctgcataagc ttcaggtaac aatgtatcta gtgtatcaac tcctgatgct aaacgttctt
    10261 taaattctat tgtcttttgt tttaacgcat catcagaata tgatttaact tcatcgctcc
    10321 atgtattgat gcgtttcact atttttctaa tcgactttag tcttaattcg tttatcgtaa
    10381 catctagttt atgtttcatt tacttcccca ccattcagtt tcgatacatc taagtaatct
    10441 aaaaatcgta ctggattcat taaacgtgac atataattta gatggttgtc ttgctcttct
    10501 ttaaaataaa actcgacatt tgtatctttt agttcattat ttcctgggac atgttctgta
    10561 agccattctt ttaaatcatc atcttcatgg cttgtacgat atactttgca atctaaatgc
    10621 tgagcgacat aagttgcaaa aacatttgac tttgacccat aactaatcaa attaatagcc
    10681 gttagggtat cttgactttg caaatcatcc tttagttgct taatattttc cttgatatcg
    10741 tcgtccatcc aacgttcaac gagccaaaca tgaccaaaca gtttcaaaaa atcattcgaa
    10801 atagttggat aggtgtcaga tggttctgca ataatgacat tgatcctatc atttccatat
    10861 tggttatcgt ctatcttcgt cacccgcatg cttttatact ctaaatcatc ttgatgcgtc
    10921 atctctgtaa ttgttaaaca tctaaatatt agactcgtcg atgctgcatt catcattttt
    10981 attttataag cataggcttc atcaggatat tgaatcgtaa tactatttga ctttacaatc
    11041 tcagtactta gttttgtgcc atttttatta taaaaaatga tgataaaata cactgaacca
    11101 gcaggcgttg catcaaaatc aaaatgcaat ttataatgct gtcctctacg caaaattggt
    11161 aaacttggcg cacttttata ttttgaaaat tgctttaaca tcaaccactc atgaatcggt
    11221 aatccagagg gcatcaaagg atttataaaa gtcacttcac catttgaaaa tgatacttta
    11281 gagccataca taaatgtagt ttgtgaaata taattccaag taactttaaa tgttttgttt
    11341 ttcagcatgt tgaactctcc caaacttgtc ttccaaaata atgttgtaaa aattaacaaa
    11401 ccaacttgca atggtaggtg aatcatcatt atgtcgccca ggaatactgc gattcatcac
    11461 tcttgcttgg tgtgctgtca atacaggtaa tagctcttga aatgcatgtg gatcataatc
    11521 atcatgttgc atatatgcta tggcaaaaac agtttgtgac aatgatttct tttgaaatgt
    11581 ttgccaaaat ttttgattta atgcctttat cgacgcttga gatgtatcac cttcattaga
    11641 caccaggacg tctaatgctg taccgaactc ttctggtcta agtaatcgca tatgttcagc
    11701 aatcgttcca atattaacaa gtggtttacc aacaataatt gcctgaggat ttaactgagc
    11761 accataatac aaggcaccaa atgaacccat agataaacca gataatatta attcatgtga
    11821 tttaaaattc agcttttcta atgtctcgtc aataacattg ataatacctt gttcatattc
    11881 agatgaaccg atataaaagc taccaccttc aacacgagga tcgccgataa gtaaaaacgg
    11941 tgcattcata cgtttcatca tataatatcc ttcgaaacct tccgctgttc gataaccact
    12001 aaaatatacg tttagtggcg gtttcatatc accagggtgg aaataataaa taaattcctg
    12061 tcgttgacta tctacgaaac gactaccacc aagtaaaaat tgacccatgt ctaatctaga
    12121 ccatcgtttg tgtataggtc ctaaatgtac cgtcccgttc ccacgcgcct taacagttac
    12181 acttatataa gcatcaaatg gtttcgcagg tatctctaaa ggactgtcta acatatcatc
    12241 agttaatatg atttgttcaa ttaatgcacc atcagcgcca gtctgaatca atctaaatgt
    12301 atattgcaac tcgaccgcac catcaatatc aaattctggc catatttgaa tgactttatc
    12361 tttatcgtaa acgagattat tttgccaaga tgcgataggt ttaaattctt ttccaaattc
    12421 tccactcaac gtgagctctg aattaccttg gtatacgaca tctactttaa aattcggatg
    12481 cacaagtgct aacttagggg aaactttatc tccatactgt cctgagaagc taactgcctc
    12541 taatttatta ttacgttctt caatattccg gtaatgtaat ggttgaacaa cgtatttttg
    12601 gacattttcg tcttgttcat attcaactga ccaaaatgat tcatcaacat acgtattgta
    12661 tggttcgctt atcatttgta ataaattcgt taatgtctcc gagtatggtg cttgaatata
    12721 gataaaatca aagcgccctt ctgcttcaac aatcgcttca atagcctcta cataaccact
    12781 atcaaattca aacaatccaa tatcgaagta atcccaactc acaccttttt tgtgttgaaa
    12841 aataggttct aaatcgtctc ctccaatttg caaaactcta aatttacgtg gcatcatttt
    12901 caccttctat taactcatcg agctgattaa taatattctt agaagcatat gcatctatta
    12961 attttaaaga ataggcgtac gcataattcc aatttttcaa ataaaataaa taataattta
    13021 acgcatcatc taattcatca actgtattta taatacggcc attgtcataa tcagagacgt
    13081 aatctgtttg ttgaccatta atttgtggaa tcccagcgct aattgcacta atttgtaaat
    13141 acaaatcagg ttcttttgac atatctatca caagtcgcaa cgtccgcaat gcttctacaa
    13201 catcatgttc agcatgtatc gtcttaacag caatgatgtc atcttgatct tcaggtgtca
    13261 ttaatgctga aacattaaca tccgcattct gtttagcttg gtattcctca tttaccgacg
    13321 taatacattc acgaagccac atcggtatgt cattttgatg gcgcgataat aaaattaaac
    13381 ggtaataatc ttcctgtgcg atataatcca caagtcgttg catcatttgt tgcaaatcag
    13441 cgtcactcat accatctatc catacaccta taaatgtttc catcaattga ctacttatat
    13501 taggtgattg tctcgtttca aatggtgtga ttcgaatcat tgtattctcc cgctgatact
    13561 tctcttgatg actaattaaa tcacgttcta gttgcacact atcaacaatt aaatgggcat
    13621 tctttacgat agattgatat tcctcatctg acacagtttc atttctattt ttaaaaaatg
    13681 aataacttaa caatttcgct ggaatatgat tggctatttg tcgattgtgc ctagcatctg
    13741 aagccacaat cacatgatca tcttcatgta tttgttgtgc aatcattgct tgaaattttt
    13801 cttcaattag ttgagccata ttgttatatt ctgtttgttg atagtgatgt tgatatcttt
    13861 ttgatacagt gactctgcca tttttcaaat cttcatgaag tacacaatct ccattaatcg
    13921 ttaaatattg ttggtaagaa gcctctccct gatcatcaaa ataacgtatc gctgataaat
    13981 aacctctgtc atcaaaaata taacgccgtt gtaactgatc tctttcaaat tcttcaaacc
    14041 aaattgaata cccttcttga ctaaaataaa tatttgtata ggtctgttca ctcgtcacac
    14101 attttaataa atacggcgtg tacacaaact caacatcatc cggccatttt aagtgatgat
    14161 aattaatcgc ttgtggcgca tggtgactga atccttgaat ttcatcaaac acagacgaat
    14221 actttgtttc atataagtca tatcgatgta aaaatgttct taaatttggt gcatgattga
    14281 gaacaatcag ttgataatct aagtcatttt caaggtgcat tcccattaaa ctaatcatat
    14341 cgtcaaattc cgtcttattt tgtagttgat aatacggcac agtcgtgtct tgccaccatc
    14401 gttggtcatc gtaccaagct ggaataaagt atttcataat tacctcctta ccaatactgg
    14461 tttaaaaatg gcttatattt atcaaaatat aaatatgtac gaattgtttc tgcaatatta
    14521 atactgatgt aaactaatac aatcagttgt actgagaaat aaatttcagt agataaatgc
    14581 ggtacaaaca atgtgaaata aagcggtata ccaataatga ctgtaactaa tgccgatcca
    14641 aaccaacata cgcgtcttgc ttgataattt aaataacgtt ctgtatcctt accaggttta
    14701 actcctgaaa aataattgcc actctttaag aaatctttgg atttttgttt agtattgatt
    14761 aaaaatctcg ataaaaaata acccaataac atttgaatca ctaaatatac tgaaatacct
    14821 actggactat caaatgtcag cattggcatg tcatctgata tgcttttatt aaacatagat
    14881 aaaataaaat gaatgccact tttcaagaaa acaaatgctg aaatactcat cattaaagta
    14941 atactgcctg cagggttaac tttccaagat aaataagatt tcatatttgt tgcggaaacg
    15001 ttcattaaat cgatatatgg tattctcact tctactaatt caatgaataa taagataaac
    15061 aatgtgatta tcacaaggat gattaacaac gcaatcacaa tatgacttgc atctatatat
    15121 tccatttttt gatgcatcat tgatttaata atgctaacca ttacaatcgg cattggtcct
    15181 gcgatgccgt aacgactatt tttgtcagct aaccaaacta ataacatcgt tccagtaacc
    15241 aaaatcaata ttgttaagta aatattgtct tgatgaacac gttctttcga aacatattca
    15301 tgaatcacaa aataactttg aatcacactt aaaattaatg ttaagatacg ctctttataa
    15361 tgcttctcaa gacttgtttg tttcatgtat ttatccatat tccgataaga aatcagcatc
    15421 aagataatca ttgatgttaa ccacggaact aatcctaaag tgaaaatgtt taaagtatta
    15481 acgtctccac ccatattaga aatagctatt ttaaaaaatg actcatgttt tacttgcata
    15541 tcgttatagg aaa
//
LOCUS       DANBWH010000044        15326 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.046, whole genome shotgun sequence.
ACCESSION   DANBWH010000044 DANBWH010000000
VERSION     DANBWH010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15326
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.046"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(84..1781)
                     /locus_tag="PPY92_000959"
     CDS             complement(84..1781)
                     /locus_tag="PPY92_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDJ2624977.1"
                     /translation="MKQLHPNEVGVYALGGLGEIGKNTYAVEYKDEIVIIDAGIKFPD
                     DNLLGIDYVIPDYTYLVQNQDKIVGLFITHGHEDHIGGVPFLLKQLNIPIYGGPLALG
                     LIRNKLEEHHLLRTAKLNEINEDSVIKSKHFTISFYLTTHSIPETYGVIVDTPEGKVV
                     HTGDFKFDFTPVGKPANIAKMAQLGEEGVLCLLSDSTNSLVPDFTLSEREVGQNVDKI
                     FRNCKGRIIFATFASNIYRVQQAVEAAIKNNRKIVTFGRSMENNIKIGMELGYIKAPP
                     ETFIEPNKINTVPKHELLILCTGSQGEPMAALSRIANGTHKQIKIIPEDTVVFSSSPI
                     PGNTKSINRTINSLYKAGADVIHSKISNIHTSGHGSQGDQQLMLRLIKPKYFLPIHGE
                     YRMLKAHGETGVECGVEEDNVFIFDIGDVLALTHDSARKAGRIPSGNVLVDGSGIGDI
                     GNVVIRDRKLLSEEGLVIVVVSIDFNTNKLLSGPDIISRGFVYMRESGQLIYDAQRKI
                     KTDVISKLNQNKDIQWHQIKSSIIETLQPYLFEKTARKPMILPVIMKVNEQKESNNK"
     gene            complement(1781..1999)
                     /locus_tag="PPY92_000960"
     CDS             complement(1781..1999)
                     /locus_tag="PPY92_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDJ2624978.1"
                     /translation="MAVFKVFYQHNRDEVIVRENTQSLYVEAQTEEQVRRYLKDRNFN
                     IEFITKLEGAHLDYEKENSEHFNVEIAK"
     gene            complement(2028..2150)
                     /locus_tag="PPY92_000961"
     CDS             complement(2028..2150)
                     /locus_tag="PPY92_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2624979.1"
                     /translation="MNDMMNLVINQIVIKLNYRDDWNEFIDIYSNTILLGLTIN"
     gene            complement(2481..3032)
                     /locus_tag="PPY92_000962"
     CDS             complement(2481..3032)
                     /locus_tag="PPY92_000962"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDJ2624980.1"
                     /translation="MLTMKDIIRDGHPTLRQKAAELELPLTKEEKETLIAMREFLVNS
                     QDEEIAKRYGLRSGVGLAAPQINISKRMIAVLIPDDGSGKSYDYMLVNPKIVSHSVQE
                     AYLPTGEGCLSVDDNVAGLVHRHNRITIKAKDIEGNDIQLRLKGYPAIVFQHEIDHLN
                     GVMFYDHIDKNHPLQPHTDAVEV"
     gene            3397..4023
                     /locus_tag="PPY92_000963"
     CDS             3397..4023
                     /locus_tag="PPY92_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkyA family protein"
                     /protein_id="HDJ2624981.1"
                     /translation="MKFGKTIAVVLASSVLLAGCTTDKKEIKAYLKQVDKIKDDEEPI
                     KTVGKKIAELDEKKKKLTEDVNSKDTAVRGKAVKDLIKNADDRLKEFEKEEDAIKKSE
                     QDFKKAKSHVDNIDNDVKRKEVKQLDDVLKEKYKLHGEYAKAYKKAVNSEKTLFKYLN
                     QNDATQQGVNEKSKAIEQNYKKLKEVSDKYTKVLNKVGKEKQDVDQFK"
     gene            4194..5306
                     /gene="pdhA"
                     /locus_tag="PPY92_000964"
     CDS             4194..5306
                     /gene="pdhA"
                     /locus_tag="PPY92_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HDJ2624982.1"
                     /translation="MAPKLQAQFDAVKVLNDTQSKFEMVQILDENGNVVNEDLVPDLT
                     DEQLVELMERMVWTRILDQRSISLNRQGRLGFYAPTAGQEASQLASQYALEKEDYILP
                     GYRDVPQIIWHGLPLTEAFLFSRGHFKGNQFPEGVNALSPQIIIGAQYIQAAGVAFAL
                     KKRGKNAVAITYTGDGGSSQGDFYEGINFAAAYKAPAIFVIQNNNYAISTPRSKQTAA
                     ETLAQKAIAVGIPGIQVDGMDALAVYQATKEARDRAVAGEGPTLIETMTYRYGPHTMA
                     GDDPTRYRTSDEDAEWEKKDPLVRFRKFLENKGLWNEDKENEVIERAKADIKAAIKEA
                     DNTEKQTVTSLMEIMYEDMPQNLAEQYEIYKEKESK"
     gene            5310..6287
                     /locus_tag="PPY92_000965"
     CDS             5310..6287
                     /locus_tag="PPY92_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017637695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDJ2624983.1"
                     /translation="MAQMTMVQAINDALKTELKNDQDVLIFGEDVGVNGGVFRVTEGL
                     QKEFGEDRVFDTPLAESGIGGLAMGLAVEGFRPVMEVQFLGFVFEVFDAIAGQIARTR
                     FRSGGTKTAPVTIRSPFGGGVHTPELHADNLEGILAQSPGLKVVIPSGPYDAKGLLIS
                     SIRSNDPVVYLEHMKLYRSFREEVPEEEYTIDIGKANVKKEGNDISIITYGAMVQESM
                     KAAEELEKDGYSVEVIDLRTVQPIDVDTIVASVEKTGRAVVVQEAQRQAGVGAAVVAE
                     LSERAILSLEAPIGRVAAADTIYPFTQAENVWLPNKNDIIEKAKETLEF"
     gene            6378..7670
                     /locus_tag="PPY92_000966"
     CDS             6378..7670
                     /locus_tag="PPY92_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDJ2624984.1"
                     /translation="MAFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKS
                     VVEIPSPVSGTVEEVMVEEGTVAVVGDVIVKIDAPDAEDMQFKGHDDDSSSKEEPAKE
                     EAPAEQAPVATQTEEVDENRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDA
                     YLNGGAPTASNESAASATSEEVAETPAAPAAVSLEGDFPETTEKIPAMRRAIAKAMVN
                     SKHTAPHVTLMDEIDVQALWDHRKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALN
                     TSFNEEAGEIVHKHYWNIGIAADTDRGLLVPVVKHADRKSIFQISDEINELAVKARDG
                     KLTADEMKGATCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPV
                     LALSLSFDHRQIDGATGQNAMNHIKRLLNNPELLLMEG"
     gene            7674..9080
                     /gene="lpdA"
                     /locus_tag="PPY92_000967"
     CDS             7674..9080
                     /gene="lpdA"
                     /locus_tag="PPY92_000967"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDJ2624985.1"
                     /translation="MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLG
                     GVCLNVGCIPSKALLHASHRFVEAQHSENLGVIAESVSLNFQKVQEFKSSVVNKLTGG
                     VEGLLKGNKVNIVKGEAYFVDNNSLRVMDEKSAQTYNFKNAIIATGSRPIEIPNFKFG
                     KRVIDSTGALNLQEVPGKLVVVGGGYIGSELGTAFANFGSEVTILEGAKDILGGFEKQ
                     MTQPVKKGMKEKGVEIVTEAMAKSAEETDNGVKVTYEAKGEEKTIEADYVLVTVGRRP
                     NTDELGLEELGVKFADRGLLEVDKQSRTSISNIYAIGDIVPGLPLAHKASYEAKVAAE
                     AIDGQAAEVDYIGMPAVCFTEPELATVGYSEAQAKEEGLAIKASKFPYAANGRALSLD
                     DTNGFVKLITLKEDDTLIGAQVVGTGASDIISELGLAIEAGMNAEDIALTIHAHPTLG
                     EMTMEAAEKAIGYPIHTM"
     gene            9248..9523
                     /locus_tag="PPY92_000968"
     CDS             9248..9523
                     /locus_tag="PPY92_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HDJ2624986.1"
                     /translation="MEYEYPIDLDWSNEEMISVINFFNHVEKYYESGVTAGDFMGAYK
                     RFKEIVPAKAEEKQIFNTFEKSSGYNSYKAVQDVKTHSEEQRVTAKK"
     gene            9667..10206
                     /locus_tag="PPY92_000969"
     CDS             9667..10206
                     /locus_tag="PPY92_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDJ2624987.1"
                     /translation="MNIGNKIKNLRRIKNLTQEELAERTDLSKGYISQIESEHASPSM
                     ETFLNIIEVLGTTPSEFFKDSENEKVLYKKEEQVIYDEYDEGYILNWLVSKSNEYDME
                     PLILTLKPGASYKNFNPSESDTFIYCMSGQITLNLGKEIYQAQEEDVLYFKARDNHRL
                     SNESNNETRILIVATASYL"
     gene            10219..11313
                     /locus_tag="PPY92_000970"
     CDS             10219..11313
                     /locus_tag="PPY92_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDJ2624988.1"
                     /translation="MEPLLSLKSVSKSYDDLNILDDIDIDIESGYFYTLLGPSGCGKT
                     TILKLIAGFEYPDSGEVIYQNKPIGNLPPNKRKVNTVFQDYALFPHLNVYDNIAFGLK
                     LKKLSKTEIDQKVTEALKLVKLSGYEKRNINEMSGGQKQRVAIARAIVNEPEILLLDE
                     SLSALDLKLRTEMQYELRELQSRLGITFIFVTHDQEEALALSDFLFVLKDGKIQQFGT
                     PTDIYDEPVNRFVADFIGESNIVEGRMVRDYVVNIYGQDFECVDMGIPENKKVEVVIR
                     PEDISLIKAEEGLFKATVDSMLFRGVHYEICCIDNKGYEWVIQTTKKAEVGSEVGLYF
                     DPEAIHIMVPGETEEEFDKRIESYEEVDNA"
     gene            11306..12103
                     /locus_tag="PPY92_000971"
     CDS             11306..12103
                     /locus_tag="PPY92_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDJ2624989.1"
                     /translation="MRNTNKFLLIPYLLWMVIFIIVPVVLLIYFSFLDINGHFSFTNY
                     QQIFTTKYLKMFAYSILYAALITIITLAISYPAAYYITRSKFQNILLMIMIIPTWINL
                     LLKTYAFIGLLSHDGVINQFFHLFNLPSFNLLFTTGAFLVVASYIYIPFMILPIFNSM
                     KAIPNNLLQASSDLGASPFYTFRKVIMPLTKEGVMTGIQVTFIPSLSLFMITRLIAGN
                     KVINIGTAIEEQFLTIQNYGMGSTIAIFLIVFMAFILIITKSSNGRG"
     gene            12109..12918
                     /locus_tag="PPY92_000972"
     CDS             12109..12918
                     /locus_tag="PPY92_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDJ2624990.1"
                     /translation="MKWYGKLYIGILLAILYIPIFFLMFYSFNSAGNMIHFEHFTLEH
                     YQSLFQNDHLMSVIFNTIAVALLAASISTVIGTFGAIAIYYLRNKKFKVTLLTLNNVL
                     MVSSDVVIGASFLIMFTTIGHFTGLGLGFWTVLISHIAFCIPIVVIIVLPQLYEMNNN
                     MLNAARDLGATEPQLLSNIIIPNILPSIIGGFFMALTYSLDDFTVSFFVTGNGFSVLS
                     VEVYAMARKGISMEINAISTLLFAVIVLGILGYYLIQYVINKKKLIKRGVK"
     gene            12918..13991
                     /locus_tag="PPY92_000973"
     CDS             12918..13991
                     /locus_tag="PPY92_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HDJ2624991.1"
                     /translation="MKRFLQLIIGALVVGMLCLTLSHWFKSKEQVHTNQKIYVYNWGE
                     YIDPELIKKFEKETGIQVVYETFDSNEAMEAKIRNGGTHYDVAFPSEYTVQKLKRDHL
                     LLPIDHNKVPNIKNLDSDYMNMSFDKGNKYSLPYFFGTVGILYNKEKYPNESFDSWKS
                     LYNPKFKNQILLVDGAREIIGMSLNKLGYNLNDRNSHHLKEAERDLTKLAPQVRGVVG
                     DEITMMLQQNEGNIAVVWSGVAAPLVQEGDKYNYVIPKEGSNLWFDNMVIPKTAQNKE
                     GAYKFMNFLLDAKNNKQNTEFVGYATPNKAARQLLPKEIKDDHRFYPTKKEQERLEVY
                     KDLGPEVLSEYNENFLNFKMSLK"
     gene            14065..15084
                     /locus_tag="PPY92_000974"
     CDS             14065..15084
                     /locus_tag="PPY92_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDJ2624992.1"
                     /translation="MTGEQFTQIKRPVSRLTEKVLGWLCWVMLLVLTVITMFIALVSF
                     SNNTSIANLENTLNNNAFIQQLLAGNGYNTTQFVIWLQNGIWAIIVYFIVCLLISFLA
                     LISMNIRILSGFLFLISAIVTIPLVLLIVTLIIPILFFIIAMMLFIRKDKVEMVAPQY
                     YEEYNGPIYDYREPVYERPQPKDDYYDVPKYEKELDKSNTVYDQEQERDKYDQFPKRA
                     VESEYNHDERTEEEPSVLSRQAKYKQKSTEELGIEDDGYYAEPEVDPKELKAQQKREK
                     AEIKAKKKEKRKAYNQRMKERRKNQPSAVSQRRMNFEERRQIYNNDISEERNSSEVKD
                     KKEQE"
BASE COUNT         5452 a         2220 c         2787 g         4867 t
ORIGIN      
        1 gctcatcata tataaagaaa aagctactaa caatgggtgg tgagtttaat taaaacttca
       61 cttcaaagtt agtagctttt tgattattta ttgtttgatt ctttttgttc gtttaccttc
      121 ataatgactg gtaaaatcat tggttttcta gctgtttttt caaataaata aggttgtaat
      181 gtttcaatga tagaagattt aatctgatgc cattgaatat ctttattttg atttaactta
      241 ctaataacat cagttttgat tttgcgttgt gcatcataaa ttaattgacc tgattccctc
      301 atatatacaa atcctcgaga aataatgtct ggaccagaaa gtaatttatt tgtattaaaa
      361 tcaatactaa caacaacgat aactaaacct tcttcagata atagcttacg gtctcttatt
      421 acaacattac cgatatcacc gataccacta ccatcaacaa gtacattacc agatggaatg
      481 cgaccagctt tacgtgctga atcgtgtgtt aaagctaaga catctccaat atcaaagatg
      541 aagacattat cttcttcaac gccgcattca acaccagtct caccatgtgc ttttaacata
      601 cggtattcac catgaatagg taagaaatat ttcggcttga ttaatcgaag cattaattgt
      661 tgatcacctt gagaaccatg ccctgaagta tggatgttag aaatcttgct atggataaca
      721 tctgcaccag ctttatacaa ggaattaata gttctgttaa tactttttgt attacctggg
      781 ataggtgatg aactaaatac aacggtatct tcaggtataa ttttaatttg cttatgagta
      841 ccattagcaa ttctagataa tgctgccatt ggttcacctt gtgaaccagt acatagtatc
      901 aataactcat gcttcggtac ggtattaatt ttattaggtt caataaatgt ttcaggtggt
      961 gctttaatat aaccaagttc cataccgatt ttaatattgt tttccatcga acgaccgaac
     1021 gtaacaattt tacggttatt tttgatagca gcttcaactg cttgttgaac tcggtaaata
     1081 ttagaagcga aggtagcaaa tataatacga cctttacaat tacggaagat cttatctacg
     1141 ttttgaccaa cttcacgttc gcttaaagta aaatcaggca caagtgaatt tgttgagtct
     1201 gaaagtaaac atagaacgcc ttcttcgcct aattgagcca ttttagcaat gtttgctggt
     1261 ttgcctacag gtgtaaaatc aaatttaaag tcaccggtat gaactacttt tccttcaggt
     1321 gtatctacga tgacgccata agtttcagga atactatgcg tagttaagta gaaagaaatc
     1381 gtaaagtgct tagatttaat cacactgtcc tcattgattt catttagttt agcagtacgt
     1441 aataaatgat gttcttcaag tttattacgg attaaaccta atgctaaagg accaccataa
     1501 ataggtatat taagttgttt taataggaag ggcacaccgc ctatatggtc ttcgtgacca
     1561 tgtgttataa ataggccaac aattttatct tggttttgaa ctagatatgt gtagtcaggt
     1621 ataacataat caatccctaa taagttatca tcagggaatt tgatacctgc atcgataatg
     1681 acaatttcgt ctttatactc aactgcataa gtatttttac cgatttcacc tagacctcca
     1741 agtgcatata cacctacttc atttggatgt aattgtttca ttatttagca atctccacat
     1801 taaagtgttc tgagttttct ttttcgtaat ctaaatgtgc gccctctaat ttagtgataa
     1861 attcgatatt aaaattacga tctttcaagt aacgacgtac ttgttcttct gtttgagctt
     1921 caacataaag tgattgtgta ttttcacgca caattacctc gtctctgtta tgttgataaa
     1981 aaactttaaa tactgccatg ttaaaaattc ctcctaagaa tgtttgttta atttattgtt
     2041 aaccctagta aaatcgtatt ggagtatata tcgataaatt cattccaatc atctctataa
     2101 tttaacttaa taacgatttg gttaataact aggttcatca tgtcgttcat tttaaaaaat
     2161 tagtgaaata acactaaaat ttcagttaaa tcttatttta catgatgaat gataataaat
     2221 aaagcagttt atctcactag aaatctaagt atttcgaata aatgtgcagt tctatttatt
     2281 aaaataaatg tagagattat aaattaatgt taagaaagaa ttaaaggatt ttatatgaaa
     2341 cggtaaaatt taaatgaggg gataaaaagt ttggtagaaa ggtgaatata tttaactgtt
     2401 gtttattcga aaaagttgta caagaaaaag cttaggacat catatttatc ctaagctttg
     2461 ataattagta aaaatgtgct ttaaacttct acagcatctg tatgtggttg taatgggtga
     2521 tttttgtcaa tgtgatcata gaacattaca ccatttaaat ggtcaatttc atgttggaaa
     2581 acaattgctg gatatccttt tagtcgtaat tgtatatcat taccttcgat gtctttggct
     2641 ttaattgtaa ttctattatg acggtgaact agaccagcaa cattatcatc gacactaagg
     2701 caaccttcac cagttggtaa ataagcttct tgaacgctat gacttacaat ttttgggttc
     2761 acaagcatat agtcataaga tttgccactg ccatcatctg gtattaaaac agcaatcata
     2821 cgtttagaaa tattaatttg aggtgcagcc aaaccaacgc ctgaacgtaa accatatcgt
     2881 ttcgcgattt cctcatcttg actatttact aaaaactctc tcatggcgat taatgtttct
     2941 ttttcttctt tagttaatgg taattctaac tcagctgctt tttgacgcaa agttggatga
     3001 ccatctctaa tgatgtcttt cattgttaac atatattgca ccttccttat tttaatttgt
     3061 tttagttgaa tgacagtaaa aaggttgtta agatactcat acatttttat gtgtaaatat
     3121 ctacaaagtt aaccaactac tgccaatgtt tattttagat agtatatgta aattttcaga
     3181 tatgctaatt gcttaaaaaa tgattaaagt gttggttcca agcaatgata ctttagaaat
     3241 ttatttatca tcttgacttt aaaaattata ttataaatga cgtaactgtc aacagatata
     3301 cttagtaatg aagatgtgta atgtaattgt ttaaaattga tttccaagca gattttattt
     3361 atcatttaat ttaaatagca agtggaggta caagtaatga aatttggaaa aacaatcgca
     3421 gtagtattag catctagtgt cttgcttgca ggatgtacta cggataaaaa agaaattaag
     3481 gcatatttaa agcaagtgga taaaattaaa gacgatgaag aaccaattaa aactgttggt
     3541 aagaaaattg ctgaattaga tgagaaaaag aaaaaattaa ctgaagatgt caatagtaaa
     3601 gatacagcag ttcgcggtaa agcagtaaaa gatttaatta aaaatgccga tgatcgtcta
     3661 aaggaatttg aaaaagaaga agacgcaatt aagaagtctg aacaagactt taagaaagca
     3721 aaaagtcacg ttgataacat tgataatgat gttaaacgta aagaagtaaa acaattagat
     3781 gatgtattaa aagaaaaata caaattgcat ggtgaatacg cgaaagcata taaaaaggct
     3841 gtaaactcag agaaaacatt atttaaatat ttaaatcaaa atgacgcgac acaacaaggt
     3901 gttaacgaaa aatcaaaagc aatagaacag aactataaaa agttaaaaga agtatcagat
     3961 aagtatacga aagtactaaa taaggttggt aaagaaaagc aagacgttga tcaatttaaa
     4021 taattaatat aatacagatg gtaggaaaca actaatacag ttcctattat ctgtatcttt
     4081 ttttattaaa acagaacttt ttcaaatggt ttaacagtcc catttatttg tggtacaatt
     4141 agtaaggata aaatgaattt ctatacaatt atgggaaagg tatggtgaat tgaatggctc
     4201 ctaagttaca agcccaattc gatgcagtaa aagttttaaa tgatactcaa tcgaaatttg
     4261 aaatggttca aattttggat gagaatggta acgtcgtaaa tgaagactta gtacctgatc
     4321 ttacggatga acaattagta gaattaatgg aaagaatggt atggactcgt atccttgatc
     4381 aacgttctat ctcattaaac agacaaggac gtttaggttt ctatgcacca actgctggtc
     4441 aagaagcatc acaattagcg tcacaatacg ctttagaaaa agaagattac attttaccgg
     4501 gatacagaga tgttcctcaa attatttggc atggtttacc attaactgaa gctttcttat
     4561 tctcaagagg tcacttcaaa ggaaatcaat tccctgaagg cgttaatgca ttgagcccac
     4621 aaattattat cggtgcacaa tacattcaag ctgctggtgt tgcatttgca cttaaaaaac
     4681 gtggtaaaaa tgcagttgca atcacttaca ctggtgacgg tggttcttca caaggtgatt
     4741 tctacgaagg tattaacttt gcagcagctt ataaagcacc tgcaattttc gttattcaaa
     4801 acaataacta tgcaatttca acaccaagaa gcaagcaaac tgctgctgaa acattagctc
     4861 aaaaagcaat tgctgtaggt attcctggta tccaagttga tggtatggat gcgttagctg
     4921 tatatcaagc aactaaagaa gcacgtgacc gcgcagttgc aggtgaaggt ccaacattaa
     4981 ttgaaactat gacatatcgt tatggtcctc atacaatggc tggtgacgat ccaactcgtt
     5041 acagaacttc agacgaagat gctgaatggg agaaaaaaga cccattagta cgtttccgta
     5101 aattccttga aaacaaaggt ttatggaatg aagacaaaga aaatgaagtt attgaacgtg
     5161 caaaagctga tattaaagca gcaattaaag aggctgataa cactgaaaaa caaactgtta
     5221 cttctctaat ggaaattatg tatgaagata tgcctcaaaa cttagcagaa caatatgaaa
     5281 tttacaaaga gaaggagtcg aagtaagcca tggcacaaat gacaatggtt caagcgatta
     5341 atgatgcgct taaaactgaa cttaaaaatg accaagatgt tttaattttt ggtgaagacg
     5401 ttggtgttaa cggcggtgtt ttccgtgtta ctgaaggact acaaaaagaa tttggtgaag
     5461 atagagtatt cgatacacct ttagctgaat caggtattgg tggtttagcg atgggtcttg
     5521 cagttgaagg attccgtccg gttatggaag tacaattctt aggtttcgta ttcgaagtat
     5581 ttgatgcgat tgctggacaa attgcacgta ctcgtttccg ttcaggcggt actaaaactg
     5641 cacctgtaac aattcgtagc ccatttggtg gtggcgtaca cacaccagaa ttacacgcag
     5701 ataacttaga aggtatttta gctcaatctc caggtctaaa ggttgttatt ccttcaggcc
     5761 catacgatgc gaaaggttta ttaatttctt ctattagaag taatgaccca gtcgtatact
     5821 tagagcatat gaaattgtat cgttcattcc gtgaagaagt acctgaagaa gaatatacaa
     5881 ttgacattgg taaggctaat gtgaaaaaag aaggtaatga catttcaatc atcacatacg
     5941 gtgcaatggt tcaagaatca atgaaagctg cagaagaact tgaaaaagat ggttattctg
     6001 ttgaagtaat tgacttacgt actgttcaac caatcgatgt tgacacaatt gtagcttcag
     6061 ttgaaaaaac tggtcgtgca gttgtagttc aagaagcaca acgtcaagct ggtgttggtg
     6121 cagcagttgt agctgaatta agtgaacgtg caatcctttc attagaagca cctattggaa
     6181 gagttgcagc agcagataca atttatccat tcactcaagc tgaaaatgtt tggttaccaa
     6241 acaaaaatga catcatcgaa aaagcaaaag aaactttaga attttaatac attttaaaag
     6301 ttaacgaagt tagcgtattt tagtctcatt gattaaaatg aaatgtttaa tttacgaaat
     6361 cttaggaggg caaaaacgtg gcatttgaat ttagattacc cgatatcggg gaaggtatcc
     6421 acgaaggtga aattgtaaaa tggtttgtta aagctggaga tactattgaa gaagacgatg
     6481 ttttagctga ggtacaaaac gataaatcag tagtagaaat cccatcacca gtatctggta
     6541 ctgtagaaga agttatggta gaagaaggta cagtagctgt agttggtgac gttattgtta
     6601 aaatcgatgc acctgatgca gaagatatgc aatttaaagg tcatgatgat gattcatcat
     6661 ctaaagaaga acctgcgaaa gaggaagcgc cagcagagca agcacctgta gctactcaaa
     6721 ctgaagaagt agatgaaaac agaactgtta aagcaatgcc ttcagtacgt aaatacgcac
     6781 gtgaaaaagg tgttaacatt aaagcagttt ctggatctgg taaaaatggt cgtattacaa
     6841 aagaagatgt agatgcatac ttaaatggtg gtgcaccaac agcttcaaat gaatcagctg
     6901 cttcagctac aagtgaagaa gttgctgaaa ctcctgcagc acctgcagca gtatcattag
     6961 aaggcgactt cccagaaaca actgaaaaaa tccctgctat gcgtagagca attgcgaaag
     7021 caatggttaa ctctaagcat actgcacctc atgtaacatt aatggatgaa attgatgttc
     7081 aagcattatg ggatcaccgt aagaaattta aagaaatcgc agctgaacaa ggtactaagt
     7141 taacattctt accttatgtt gttaaagcac ttgtttctgc attgaaaaaa tacccagcac
     7201 ttaacacttc attcaatgaa gaagctggtg aaattgtgca taaacattat tggaatatcg
     7261 gtattgcggc ggacactgat agaggtttat tagtacctgt agtgaaacat gctgatcgta
     7321 agtctatttt ccaaatttca gatgaaatta atgaattagc tgttaaagca cgtgatggta
     7381 aattaacagc cgatgaaatg aaaggtgcaa catgcacaat cagtaatatc ggttcagctg
     7441 gtggacaatg gttcactcca gttatcaatc acccagaagt agcaatctta ggaattggcc
     7501 gtattgctca aaaacctatc gttaaagatg gagaaattgt tgcagcacca gtattagcat
     7561 tatcattaag ctttgaccac agacaaattg atggtgcaac tggccaaaat gcaatgaatc
     7621 acattaaacg tttattaaat aatccagaat tattattaat ggaggggtaa aacatggtag
     7681 ttggagattt cccaattgaa acagatacta tagtaatcgg agcaggtcct ggtggatacg
     7741 ttgcagcaat tcgtgcagct caattaggac aaaaagtaac aatcgttgag aaaggtaatc
     7801 ttggtggtgt ttgcttaaac gtaggatgta ttccttcaaa agcattacta catgcttctc
     7861 accgttttgt tgaagcacaa cattctgaaa acttaggtgt tattgctgaa agtgtttctt
     7921 taaacttcca aaaagttcaa gaattcaaat catcagttgt taataaatta actggtggtg
     7981 ttgaaggctt acttaaaggt aacaaagtta acatcgttaa aggtgaagca tatttcgtag
     8041 ataacaatag cttacgtgtt atggacgaaa agagcgcaca aacatacaac tttaaaaatg
     8101 caatcattgc aacaggttca agaccaattg aaattcctaa tttcaaattc ggtaaacgtg
     8161 ttatcgactc aacaggtgct ttaaacttac aagaagtacc aggtaaatta gttgtagttg
     8221 gtggaggata cattggatca gaattaggta cagcatttgc taactttggt tcagaagtaa
     8281 ccatccttga aggtgctaaa gatatcttag gtggcttcga aaaacaaatg acacaacctg
     8341 ttaaaaaagg tatgaaagaa aaaggtgttg aaatcgttac tgaagctatg gctaaatcag
     8401 ctgaagaaac agataacgga gttaaagtta cttatgaagc taaaggcgaa gagaaaacaa
     8461 tcgaagctga ttatgtatta gtaactgtag gtcgtcgtcc aaacacagac gaattaggcc
     8521 tagaagaatt aggtgttaaa ttcgctgacc gtggattatt agaagttgat aaacaaagcc
     8581 gtacgtctat cagcaatatc tatgcaattg gtgatatcgt tccaggttta ccacttgctc
     8641 acaaagctag ctatgaagct aaagttgctg ctgaagcaat tgatggtcaa gctgctgaag
     8701 ttgattacat tggtatgcca gcagtatgct ttactgaacc agaattagct acagttggtt
     8761 attcagaagc gcaagctaaa gaagaaggtt tagcaattaa agcttctaaa ttcccatatg
     8821 cagcaaatgg tcgtgcatta tcattagatg atactaacgg atttgttaaa cttattacac
     8881 ttaaagaaga tgatacttta atcggtgctc aagtagttgg tactggtgca tcagatatta
     8941 tctctgaatt aggtttagca attgaagctg gtatgaatgc tgaagatatc gcattaacaa
     9001 tccatgcaca tccaacatta ggtgagatga ctatggaagc agcagaaaaa gctatcggat
     9061 acccaatcca tacaatgtaa taactgatta tctataaaga ttcagtcatt aaaagctgta
     9121 gcatatgcta cggctttttt gttttaggta aagtaatgta aggaaattga tttgagatat
     9181 cgttaacatg tgacatgcat gttatactag cgatgctaac aaaagaattg aaatggaggg
     9241 ttcaacaatg gaatatgagt atccaattga tttagactgg agtaatgaag agatgatttc
     9301 agtgataaat ttctttaatc atgtagagaa gtattatgaa tccggcgtga cggcaggcga
     9361 ctttatgggt gcatataaaa gatttaaaga aattgtgcct gctaaagcag aggaaaaaca
     9421 aatttttaat actttcgaaa aaagtagtgg ctataatagt tacaaagcag ttcaagatgt
     9481 aaaaactcac tctgaagaac aaagagtaac agctaaaaaa taattcgttc gaaattaaca
     9541 caatttaata ggattttttc tttaaaacta ttgctaataa agctatattt tgataccttt
     9601 atcaagtgtt aaacaaaatg tttgataaaa gtaaacttaa tatagctttt ttaggtggaa
     9661 aaataaatga acataggtaa taaaattaaa aatcttagaa gaattaaaaa tttaacgcaa
     9721 gaagaacttg ctgaacgtac agacttatcg aaaggctaca tttcacaaat agaaagtgaa
     9781 catgcctcac caagtatgga aactttctta aatattatag aggtgttagg aacgacgcca
     9841 agtgaatttt ttaaagacag tgaaaatgaa aaagtattat acaagaagga agaacaagtt
     9901 atttatgatg agtatgatga aggttatata ttaaattggt tagtttcaaa gtcaaatgaa
     9961 tatgatatgg agccattaat attaacttta aagcctggag catcatataa aaattttaat
    10021 ccatcagagt ctgatacgtt tatttattgt atgtcaggtc agataacact taatttaggc
    10081 aaagagatat atcaagcaca agaagaagac gttttgtatt ttaaagcacg agataatcat
    10141 cgtttgtcaa acgaatcaaa caatgaaaca cgaatactta ttgtagcgac agcttcatat
    10201 ttataggggg gatcttattt ggaaccgtta ttatcattaa aatcagttag taaaagctat
    10261 gatgatctta atatcttaga tgacatagat attgatattg aatcaggata cttttataca
    10321 ttattaggtc cttcaggttg tggtaaaaca acaattttaa aattaattgc agggtttgaa
    10381 tatcctgaca gtggtgaagt gatttatcaa aacaaaccaa ttggtaattt accaccaaat
    10441 aaacgtaaag tgaatacagt ctttcaagat tatgcattat ttccacactt aaacgtatat
    10501 gataatatcg cttttggttt gaaattaaaa aaattatcaa aaaccgaaat tgatcaaaaa
    10561 gtaactgagg cattaaaatt agtaaaactt tcaggttatg aaaaaagaaa tattaatgaa
    10621 atgagtggcg gacaaaagca acgtgttgca attgcacgtg ctatcgtaaa tgaaccagaa
    10681 atattattgt tagatgaatc tttatccgca ttagatttga aattgcgtac tgaaatgcaa
    10741 tatgaattac gagaattgca atctagatta ggtattacat ttatatttgt aacacatgat
    10801 caagaagaag cgttagcatt aagtgacttt ctttttgtat taaaagatgg gaaaattcaa
    10861 caatttggca caccaacaga tatatatgac gaaccagtga atcgatttgt agctgatttt
    10921 attggagaat ctaatattgt tgaaggacgc atggttagag attatgtcgt gaatatttat
    10981 gggcaagatt tcgaatgtgt cgatatgggt attcctgaaa ataaaaaagt agaagtcgtt
    11041 attcgaccag aagatatatc attaatcaaa gctgaagaag gattatttaa agcaactgtt
    11101 gattctatgt tatttagagg ggtccactat gaaatatgtt gtatagacaa taaaggttat
    11161 gaatgggtaa tacaaacgac taaaaaagct gaagtaggca gtgaagttgg tctttatttt
    11221 gatcctgaag ccattcatat tatggttcct ggagaaacag aagaagaatt tgataaacgt
    11281 attgaaagct atgaggaagt agacaatgcg taatactaat aaatttctct taatcccgta
    11341 tttactatgg atggttatat ttattattgt accagttgta ttactcattt atttttcatt
    11401 tttagatatc aatggacatt ttagtttcac gaattatcaa caaattttta ctacaaaata
    11461 tttgaaaatg tttgcatatt caattttata tgccgcttta ataacaatta ttacgttggc
    11521 tatcagttat ccagctgcct attatattac tcgttcgaaa tttcaaaata tcttattaat
    11581 gataatgatt attccaacat ggataaattt attgttaaag acatatgctt ttataggttt
    11641 attaagtcat gatggcgtga ttaatcaatt tttccactta tttaatttac catcattcaa
    11701 tttgttattt acaactggtg cgtttttagt agtggcaagt tacatttata taccatttat
    11761 gattttacct atatttaata gcatgaaagc aattcctaat aatttattgc aggcctcaag
    11821 tgatttaggt gccagtcctt tctatacttt tagaaaagta atcatgccgt taacaaaaga
    11881 aggtgttatg actgggattc aagtaacctt tattccatca ctttcattat ttatgattac
    11941 aagattaatt gcaggtaata aagtcataaa tataggtacg gcaatagagg aacaattttt
    12001 aacaattcaa aattatggta tgggatcaac tatagctata ttcttaattg tatttatggc
    12061 attcatttta atcattacaa aatcatctaa tgggagaggg tgaatcatat gaaatggtat
    12121 ggaaagctgt atatcgggat acttttagcg attttataca tcccaatatt ctttttaatg
    12181 ttctattcat tcaattcggc tggtaatatg attcactttg aacattttac attagagcat
    12241 tatcaatcat tatttcaaaa tgatcattta atgtcggtca tttttaatac gatagctgta
    12301 gcacttttag cagcctcaat ttctacagtt attggtacat ttggtgccat tgctatttat
    12361 tatttaagaa ataaaaagtt taaagtaact ttactaacat tgaataatgt cttgatggta
    12421 tcatccgacg ttgtcatagg tgcatcattc ttaattatgt ttacaacgat tggccatttt
    12481 actggtcttg gtttaggatt ttggacagtt ctaatatctc atattgcatt ttgcatacct
    12541 atagttgtga ttatcgtctt accacaactg tatgaaatga ataataatat gttaaatgct
    12601 gcaagagatt taggagcgac tgaaccacaa ttattaagca acattattat tcctaatatt
    12661 ttaccttcta ttataggagg tttctttatg gctttaactt attcactaga cgatttcaca
    12721 gtaagtttct tcgttactgg taatggcttt agtgtgttat cagttgaagt ttatgctatg
    12781 gcgagaaaag gaattagtat ggaaattaat gcgatttcaa cattattgtt tgctgttatt
    12841 gtattaggaa tactaggata ttatttgatt caatacgtga taaataagaa aaaactaatc
    12901 aagcgaggtg taaaataatg aaacgttttt tacaactcat tataggtgca ttagttgtgg
    12961 gtatgctttg tcttacttta agtcattggt ttaaatctaa agaacaagtg catacaaatc
    13021 aaaaaattta cgtatacaat tggggcgaat atattgatcc agagttaatt aagaaatttg
    13081 aaaaagaaac tggcattcaa gtcgtttatg aaactttcga ttcaaatgaa gcgatggaag
    13141 ccaaaattcg caatggcggt acacattatg atgttgcttt tcctagtgaa tatacagttc
    13201 aaaaattgaa aagagatcat ttattgttac caatagatca taataaggta cctaatatta
    13261 aaaatttaga ttcagattat atgaatatgt catttgataa aggcaataaa tattctttac
    13321 cttatttctt tggaactgta ggtattttat ataataaaga aaagtatcca aatgaatcat
    13381 ttgatagttg gaagtcattg tataatccta aatttaaaaa ccaaatttta ctagttgacg
    13441 gtgctagaga gattataggc atgagtttga ataaacttgg gtataatctt aatgaccgta
    13501 attcgcacca tttaaaagaa gcagagcgag atttaaccaa actagcacca caagtaagag
    13561 gtgtcgtagg tgatgaaatt accatgatgc ttcaacaaaa tgaaggtaac atagcggttg
    13621 tttggagtgg tgttgcagca cctctagtgc aagaagggga taaatataat tatgttattc
    13681 ctaaagaagg atcgaattta tggttcgaca atatggtaat tccaaaaacg gcacaaaata
    13741 aagagggtgc atataaattt atgaattttt tattagatgc taaaaataac aagcaaaata
    13801 cagaattcgt aggctatgca acgccaaaca aggctgctcg acaattgttg cctaaagaga
    13861 ttaaagacga ccatcgtttt tatccgacta agaaagagca agaacgcctt gaagtttata
    13921 aagatttagg acctgaagtt ttaagtgaat acaatgaaaa ctttttgaat ttcaaaatgt
    13981 cattaaaata aaatcataaa tagtagttat ttactgtata tttagttata ataacgggat
    14041 aagtactata aggagttgta acgaatgact ggagaacaat ttactcaaat taaacgtcca
    14101 gtaagtagat taactgaaaa agttctaggt tggttatgtt gggtgatgtt attagtgctt
    14161 actgtcatta cgatgtttat agcacttgtt tcattcagta ataacacatc gatagctaat
    14221 cttgaaaata cattaaacaa taatgcattt atccagcaat tattagctgg aaatggctat
    14281 aatacaacac aatttgtaat atggttacaa aatggtatat gggctattat cgtttacttt
    14341 attgtttgtt tgttgatttc atttttagct ctcatatcta tgaatataag aatcttgtca
    14401 ggtttcttat tcttaatatc agcaattgta acgattcctt tagttttact tatcgttact
    14461 ttaatcattc cgatattatt ctttattatt gcgatgatgc tatttataag aaaagataaa
    14521 gttgaaatgg ttgcgccaca atattatgaa gagtataacg gaccgattta tgattatcgt
    14581 gaacctgtgt atgagcgccc ccaaccgaaa gatgattatt atgatgtgcc taaatatgaa
    14641 aaagaattgg ataaatcaaa tactgtatat gatcaagaac aggaaagaga taaatatgat
    14701 caatttccta aacgtgcagt tgaaagtgaa tataatcatg atgagcgcac tgaggaagaa
    14761 ccatcagtat tatccagaca ggctaaatac aaacaaaaaa gtactgaaga actaggtatt
    14821 gaagatgatg gttattatgc agaacctgaa gttgatccaa aagaattgaa agcacaacaa
    14881 aagcgagaaa aagctgaaat taaagctaag aaaaaagaaa aacgtaaagc ttataaccaa
    14941 cgaatgaaag aacgtaggaa aaatcaacct agcgcagtta gtcaacgtcg aatgaatttt
    15001 gaagagcgac gtcaaattta caacaatgat atttctgaag aacgcaattc aagtgaagtt
    15061 aaggacaaaa aagagcaaga ataaatattg atgataccca gattagtaag tagaggtctt
    15121 ttgcttatta atctgggttt ttatatgagg ttaatcatcg ataacgttta attaaagtgt
    15181 ttaggtgtca aaattttaaa tgacgatttc tcattaccat acacctaaat tatcatcaat
    15241 ctgaattcag atgtttatta taaaaattag atgaaaaata tgttaatata caagtaattt
    15301 aatgtgcgta tatctatagg ctcgta
//
LOCUS       DANBWH010000045        15323 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.047, whole genome shotgun sequence.
ACCESSION   DANBWH010000045 DANBWH010000000
VERSION     DANBWH010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15323
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.047"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            29..2125
                     /gene="pnp"
                     /locus_tag="PPY92_000975"
     CDS             29..2125
                     /gene="pnp"
                     /locus_tag="PPY92_000975"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDJ2624993.1"
                     /translation="MSQEKKVFKTEWAGRSLTIETGQLAKQANGAVLVRYGDTVVLST
                     ATASKEPRDGDFFPLTVNYEEKMYAAGKIPGGFKKREGRPGDDATLTARLIDRPIRPL
                     FPKGYKHDVQIMNMVLSADPDCSPQMAAMIGSSMALSVSDIPFQGPIAGVNVGYIDGK
                     YIINPTVEEKEVSRLDLEVAGHKDAVNMVEAGASEITEQEMLEAIFFGHEEIQRLVDF
                     QQQIVDHIQPVKQEFIPAERDEALVERVKSLTEEKGLKETVLTFDKQQRDENLDNLKE
                     EIVNEFIDEEDPENELLIKEVYAILNELVKEEVRRLIADEKIRPDGRKPDEIRPLDSE
                     VGILPRTHGSGLFTRGQTQALSVLTLGALGDYQLIDGLGPEEEKRFMHHYNFPNFSVG
                     ETGPVRAPGRREIGHGALGERALKYIIPDTADFPYTIRIVSEVLESNGSSSQASICGS
                     TLALMDAGVPIKAPVAGIAMGLVTREDSYTILTDIQGMEDALGDMDFKVAGTKEGITA
                     IQMDIKIDGLTREIIEEALEQARRGRLEIMNHMLQTIDQPRTELSAYAPKVVTMTIKP
                     DKIRDVIGPGGKKINEIIDETGVKLDIEQDGTIFIGAVDQAMINRAREIIEEITREAE
                     VGQTYQATVKRIEKYGAFVGLFPGKDALLHISQISKNRIEKVEDVLKIGDTIEVKITE
                     IDKQGRVNASHRALEE"
     gene            2361..4034
                     /locus_tag="PPY92_000976"
     CDS             2361..4034
                     /locus_tag="PPY92_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDJ2624994.1"
                     /translation="MSLIKKKNKDIRIIPLGGVGEIAKNMYIVEVDDEMFMLDAGLMF
                     PEDEMLGIDIVIPDISYVLENKDKLKGIFLTHGHEHAIGAVSYVLEQLDAPVYGSKLT
                     IALIKENMKARNIDKKVRYYTVNNDSIMRFKNVNISFFNTTHSIPDSLGVCIHTSYGT
                     IVYTGEFKFDQSLHGHYAPDIKRMAEIGEEGVFVLISDSTEAEKPGYNTPENVIEHHM
                     YDAFAKVRGRLIVSCYASNFIRIQQVLNIASKLNRKVSFLGRSLESSFNIARKMGYFD
                     IPKDLLIPITEVDNYPKNEVIIIATGMQGEPVEALSQMAQHKHKIMNIEEGDSVFLAI
                     TASANMEVIIANTLNELVRAGAHIIPNNKKIHASSHGCMEELKMMINIMKPEYFIPVQ
                     GEFKMQIAHAKLAAEAGVAPEKIFLVEKGDVINYNGKDMILNEKVNSGNILIDGIGIG
                     DVGNIVLRDRHLLAEDGIFIAVVTLDPKNRRIAAGPEIQSRGFVYVRESEDLLREAEE
                     KVREIVEAGLQEKRIEWSEIKQNMRDQISKLLFESTKRRPMIIPVISEI"
     gene            4291..6657
                     /locus_tag="PPY92_000977"
     CDS             4291..6657
                     /locus_tag="PPY92_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDJ2624995.1"
                     /translation="MAQAKKKSTAKKKTASKKRTNSRKKKNDNPIRYVIAILVVVLMV
                     LGVFQLGIIGRLIDSFFNYLFGYSRYLTYILVLLATGFITYSKRIPKTRRTAGSIVLQ
                     IALLFVSQLVFHFNSGIKAEREPVLSYVYQSYQHSHFPNFGGGVLGFYLLELSVPLIS
                     LFGVCIITILLLCSSVILLTNHQHRDVAKVALENIKAWFGSFNEKMSERNQEKQLKRE
                     EKARLKEEQKARQNEQPQIKDVSDFTEVPQERDIPIYGHTENESKSQCQPSRKKRVFD
                     AENSSNNIVNHQADQQEQLTEQTHNSVESENTIEEAGEVTNVSYVVPPLTLLNQPAKQ
                     KATSKAEVQRKGQVLENTLKDFGVNAKVTQIKIGPAVTQYEIQPAQGVKVSKIVNLHN
                     DIALALAAKDVRIEAPIPGRSAVGIEVPNEKISLVSLKEVLDEKFPSNNKLEVGLGRD
                     ISGDPITVPLNEMPHLLVAGSTGSGKSVCINGIITSILLNAKPHEVKLMLIDPKMVEL
                     NVYNGIPHLLIPVVTNPHKAAQALEKIVAEMERRYDLFQHSSTRNIKGYNELIRKQNQ
                     ELDEKQPELPYIVVIVDELADLMMVAGKEVENAIQRITQMARAAGIHLIVATQRPSVD
                     VITGIIKNNIPSRIAFAVSSQTDSRTIIGTGGAEKLLGKGDMLYVGNGDSSQTRIQGA
                     FLSDQEVQDVVNYVVEQQQANYVKEMEPDAPVDKSEMKSEDALYDEAYLFVVEQQKAS
                     TSLLQRQFRIGYNRASRLMDDLERNQVIGPQKGSKPRQVLIDLNNDEV"
     gene            6662..7375
                     /locus_tag="PPY92_000978"
     CDS             6662..7375
                     /locus_tag="PPY92_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDJ2624996.1"
                     /translation="MSEMNAVYNVKQYILNLIMQNKLEYGDQLPSNLSIARELNVKTD
                     DVYEAIQALITEQVIKDNFEEGTSVKSLPPFFYPLNELISIGQMIKNAGFECGTEYLN
                     FDEQPATMLDANLLSVEEGYPVTIIERLRTADGEPVVYCLDKIAKKELTCTEYQMSNG
                     SILSAIKEQSNHNICYADTEIEAVNYEPRISEVLNASPHEGLILLKITHYNELDEPIL
                     YSLNYMKNSLVQFKITRKI"
     gene            7406..8671
                     /locus_tag="PPY92_000979"
     CDS             7406..8671
                     /locus_tag="PPY92_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDJ2624997.1"
                     /translation="MSRQSQPNIHIKVSPTTKFKTTTIVFKFMAPLEYDTITARSLLS
                     KLLVRATKKWPTDKSFNNHLADLYGAYVNSTISKFKDQHVITFSLEIVNERYLRNGES
                     LFNQGLDLLQEIIWNPLIENKAFNDNFVNQEKTLLAKKIEAMVDNKAQYSFLKLLDHM
                     FENEAYKYLSTGQLEQIPHITAETLYHTYQSMINNDQCSVYVVGNVEPESVEKQIREK
                     FALKPFDKHQFQHSTHHLHDEEVDYIVEYDDVDQAKLNMGYRFPTQYGQSGYAAFVVF
                     NMMFGGDPSSVLFNEVREKQSLAYSIHSQIDGKNGYLFVLSGVSSDKYETAKDTIISE
                     FEKIKAGDFTEEKLELAKKVIISHRYESEDRPKSIIEIMHNQILLEQPQSKETFINDI
                     QKVSREDIVSVAEKAFLDTIYVLTKGGDK"
     gene            8671..9957
                     /locus_tag="PPY92_000980"
     CDS             8671..9957
                     /locus_tag="PPY92_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDJ2624998.1"
                     /translation="MKERYYELIDERVFEQELENGLRLFIIPKPGFQKTFVTYTTQFG
                     SLDNQFKPLGQDQFVTVPDGVAHFLEHKLFEKEEEDLFTAFAEDNAQANAFTSFDRTS
                     YLFSATDNIENNIKRLLTMVETPYFTKETVDKEKGIIAEEIKMYQEQPGYKLMFNTLR
                     AMYQRHPIRVDIAGSVESIYDITKDDLYLCYETFYHPSNMVLFVVGDVNPEEICRIVK
                     QHEDARNKVNQPKIERGLVDEPEDVKEAFVTESMKIQSPRLMLGFKNKPLQEAPQKYV
                     QRDLEMSLFFELIFGEETDFYQNLLNEGLIDDTFGYQFVLEPTYSFSIVTSATEEPDK
                     LKKLLLDELRDKKGNFQDAEAFELLKKQFIGEFISSLNSPEYIANQYTKLYFEGVSVF
                     DMLDIVENITLDSINETSSLYLNLDQQVDSRLEIKK"
     gene            9957..10661
                     /locus_tag="PPY92_000981"
     CDS             9957..10661
                     /locus_tag="PPY92_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDJ2624999.1"
                     /translation="MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTSKFN
                     DDKVRFIQADLSQTIDIDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNV
                     LQLIRLCRYFVDVLRQSANGRIIVISSIWGETGASMETIYSAMKSAQLGFVKALSQEL
                     ALTSVTVNAIAPGFVAGNMASEWQEDELQAMITELPQQRLVLPSEVAHTCAYLYHSNA
                     RSVTGTIQKVNGAWYI"
     gene            10766..11593
                     /locus_tag="PPY92_000982"
     CDS             10766..11593
                     /locus_tag="PPY92_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfK family protein"
                     /protein_id="HDJ2625000.1"
                     /translation="MTVAEKKEWYLEYEIEINRPGLLGDVSSLLGMLGISIVTINGVD
                     QGKRGLLIKTDNLEKVERFEQIARGINEIEITKLKKPELRDRLAVRHGRYIEQDAKDK
                     KTFRFEREDLGLLVDFLAELFKEEGHKLIGIRGMPRVGKTESIVAGSVCAHKRWLFIS
                     STLIKQTVRSSLIKGEYDANHVYIIDGAVTARESNPKHQELVNEVMTLPSIKVVEHPD
                     LFVETSTCTMEDFDYIIELRENENQEIHYEEMKKQTVQSKNNLDFGDPFGGGFGFFE"
     gene            11612..12004
                     /locus_tag="PPY92_000983"
     CDS             11612..12004
                     /locus_tag="PPY92_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDJ2625001.1"
                     /translation="MKTVGEALKGRRERLGMTLTELEQRTGIKREMLVHIENNEFDQL
                     PNKNYSEGFIRKYASVVNIEPNQLIQAHQDEIPSNQAEWDEVITVFNNNKDLDYKSKS
                     KEPIQLLVIMGITVLITLLLWIMLVLIF"
     gene            12038..12616
                     /gene="pgsA"
                     /locus_tag="PPY92_000984"
     CDS             12038..12616
                     /gene="pgsA"
                     /locus_tag="PPY92_000984"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDJ2625002.1"
                     /translation="MNIPNQITVFRVVLIPVFILFALVDFGFGNVSFLGGYEIRIELL
                     ISGFIFILASLSDFVDGYLARKWNLVTNMGKFLDPLADKLLVASALIVLVQLGLTNSV
                     VAIIIIAREFAVTGLRLLQIEQGFVSAAGQLGKIKTAVTMVAITWLLLGDPLATLIGL
                     SLGQILLYIGVIFTILSGIEYFYKGRDVFKQK"
     gene            12840..13991
                     /locus_tag="PPY92_000985"
     CDS             12840..13991
                     /locus_tag="PPY92_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HDJ2625003.1"
                     /translation="MSIAIIAVGSELLLGQIANTNGQFLSKVFNEIGQNVLEHKVIGD
                     NKKRLESSVRHALEKYDTVILTGGLGPTKDDLTKHTVAQIVGKDLVIDEPSLKYIESY
                     FEEQGQEMTPNNKQQALVIEGSTVLANHHGMAPGMMVNFENKQIILLPGPPKEMQPMV
                     KNELLSHFINHNRIIHSELLRFAGIGESKVETILIDLIDKQTNPTIAPLAGSHEVYIR
                     LTANADSKEQAQSLIQPVKQEILDRIGEYYYGSDDTLIEQAVIKKIHEPFVIYDGITN
                     GALYHRLKEVDLNDVLKGMINHNENFVDINKPIEQQLKDAVQFVNKLFNVSSAIILLE
                     YDGVVHIGYDNNFEFKTEQFKMSKSRNLLKNRSQNYVLIRLLNWLRTTN"
     gene            14156..15199
                     /gene="recA"
                     /locus_tag="PPY92_000986"
     CDS             14156..15199
                     /gene="recA"
                     /locus_tag="PPY92_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDJ2625004.1"
                     /translation="MDNDRQKALDTVIKNMEKSFGKGAVMKLGDNIGRRVSTTSTGSV
                     TLDNALGVGGYPKGRIIEIYGPESSGKTTVALHAIAEVQSNGGVAAFIDAEHALDPEY
                     AQALGVDIDNLYLSQPDHGEQGLEIAEAFVRSGAVDIVVVDSVAALTPKAEIEGEMGD
                     THVGLQARLMSQALRKLSGAISKSNTTAIFINQIREKVGVMFGNPETTPGGRALKFYS
                     SVRLEVRRAEQLKQGQEIVGNRTKIKVVKNKVAPPFRVAEVDIMYGQGISKEGELIDL
                     GVENDIVDKSGAWYSYNGERMGQGKENVKMYLKENPQIKEEIDRKLREKLGISDGDVE
                     ETEDAPKSLFDEE"
BASE COUNT         5575 a         2052 c         2972 g         4724 t
ORIGIN      
        1 ctattagatt caagagagga gatttataat gtctcaagaa aagaaagttt ttaaaactga
       61 atgggcagga agatctttaa cgattgaaac agggcaatta gctaaacaag caaatggcgc
      121 tgtattggtt cgttatggag atacagtcgt gttatcgacg gcaactgcat caaaagaacc
      181 tcgtgatgga gatttcttcc cattaacagt gaactacgaa gaaaaaatgt acgctgcggg
      241 taaaattcct ggtggattta aaaagagaga aggacgtcct ggtgacgatg caacattaac
      301 tgcgcgatta attgatagac caattagacc tttattccct aaagggtata agcatgatgt
      361 tcaaattatg aacatggtat taagtgcaga tcctgattgt tcaccacaaa tggctgcaat
      421 gattggttca tctatggcgc ttagtgtgtc ggatattcca ttccaagggc caatcgccgg
      481 tgtaaatgtg ggttatattg atggtaaata tatcattaac ccaacagtag aagaaaaaga
      541 agtttctcgt ttagaccttg aagtagctgg tcataaagat gcagtaaaca tggtagaggc
      601 aggcgctagt gagattactg aacaagaaat gttagaggcg attttctttg gtcatgaaga
      661 gattcaacgt ttagttgatt tccaacaaca aatcgtcgac cacattcaac ctgttaaaca
      721 agaatttatt ccagcagagc gtgatgaagc gctagttgaa cgtgtgaaat ctttaaccga
      781 agaaaaggga cttaaagaaa cagttttaac atttgataaa caacaacgag atgaaaatct
      841 tgataactta aaagaagaaa tcgtcaatga atttatcgat gaagaagatc cagagaatga
      901 attacttatt aaagaagttt atgcaatttt aaatgaatta gtgaaagaag aagttcgacg
      961 tttaattgca gatgaaaaaa ttagaccaga cggccgtaaa cctgatgaaa tccgtccatt
     1021 agattctgaa gttggtattt tacctagaac gcatggttca ggtctattta cacgtggtca
     1081 gactcaagca ctttcagttt taacattagg tgctttaggc gattatcaat taattgatgg
     1141 tttaggacct gaagaagaaa aaagattcat gcatcattat aacttcccga acttttcagt
     1201 aggtgaaact ggtccagtac gtgcgccagg tcgtcgtgaa attggacatg gtgcgttagg
     1261 tgaaagagca ttaaaatata ttattcctga tactgctgat ttcccatata caattcgtat
     1321 tgtaagtgag gtacttgaat caaacggttc atcatctcaa gcgtcaattt gtggatcaac
     1381 cttagcatta atggatgcgg gcgtaccgat taaagcacca gttgctggta ttgctatggg
     1441 ccttgttaca cgtgaagata gctatacgat tttaactgat atccaaggta tggaagatgc
     1501 attaggtgat atggacttta aagtcgctgg tactaaagaa ggtattacag caatccaaat
     1561 ggatattaaa attgacggtt taacgcgtga aattatcgaa gaggctctag aacaagcgag
     1621 acgtggtcgt ttagaaataa tgaatcatat gttacaaaca attgatcaac cacgtactga
     1681 attaagtgct tacgcgccaa aagttgtaac tatgacaatt aaaccagata agattagaga
     1741 tgttatcgga cctggtggta aaaaaattaa cgaaattatt gatgaaacag gcgttaaatt
     1801 agatattgaa caagatggta ctatctttat tggggccgtt gatcaagcta tgataaatcg
     1861 tgctcgtgaa atcattgagg aaattacacg tgaagcggaa gtaggtcaaa cttatcaagc
     1921 cactgttaaa cgtattgaaa aatacggtgc gtttgtaggc ctattcccgg gtaaagatgc
     1981 gttgcttcac atttcacaaa tttcaaaaaa tagaattgaa aaagtggaag atgttttaaa
     2041 aatcggtgac acaattgaag ttaagattac tgaaattgat aaacaaggtc gagtaaatgc
     2101 atcacacaga gcattagaag aataatattt aaagtcatat gacgacaatg tatcgtcatg
     2161 tgattttttt atgccatttt ttacgaagtg atccattttg aatttgttgt attgaacatt
     2221 ttaaaacgct ttattatttt gagtgcaact gttaattatc ctgtatgtat agtgattaat
     2281 agtgtacatc aagtgttttt taacttataa tgaatagtga gtttatatat ggacgggtaa
     2341 caaatttagg aggtaagatt ttgagtttaa taaagaaaaa gaataaagat attcgcatta
     2401 taccattagg cggtgttggc gaaattgcta aaaatatgta tatcgttgaa gtagacgatg
     2461 aaatgtttat gttagatgct ggacttatgt ttccagaaga cgaaatgcta ggtattgata
     2521 ttgttatacc agacatttca tacgtacttg aaaataaaga taaattgaag ggtatattcc
     2581 ttacacacgg acatgagcac gcgattggtg cagtgagtta tgttttagaa caattagatg
     2641 caccagtata tggatctaaa ttgacaatag cgttaattaa agaaaatatg aaagcccgta
     2701 atattgataa aaaagttcgc tactacacag ttaacaatga ttcaattatg agattcaaaa
     2761 acgtgaatat tagtttcttt aatacgacac acagtattcc tgatagttta ggtgtttgta
     2821 ttcacacttc atatggtacc attgtgtata caggtgaatt taagtttgac caaagtttac
     2881 atggacatta tgcaccagat attaaacgta tggcagagat tggtgaagaa ggcgtatttg
     2941 tcttaatcag tgattctact gaggcagaga aacctggata taatactccg gaaaatgtaa
     3001 ttgaacatca tatgtatgat gcttttgcaa aagtgcgagg tcgcttgatt gtttcatgtt
     3061 atgcttcgaa ctttatacgt attcagcaag ttttaaatat tgctagcaag ctaaatcgta
     3121 aagtgtcatt tttaggaaga tcacttgaaa gttcatttaa tattgctcgt aaaatggggt
     3181 atttcgacat tcctaaagat ttgctaattc ctataacaga agttgataat tatcctaaaa
     3241 atgaagtgat aattatagct actggtatgc aaggagaacc tgtagaagcc ttaagtcaaa
     3301 tggcgcaaca taagcataaa attatgaata tcgaagaagg cgattctgta tttttagcaa
     3361 ttacggcttc tgctaatatg gaagttatca ttgcgaatac attaaatgag cttgtacgtg
     3421 ctggcgcaca tattattcca aataacaaga agattcatgc ttcaagtcat ggttgcatgg
     3481 aagaattaaa aatgatgatt aatattatga aacctgaata ctttattcct gtacaaggtg
     3541 aatttaaaat gcagatagca catgcgaagc tagcagctga agcaggtgtt gcaccggaaa
     3601 agattttcct tgtggaaaaa ggagatgtca ttaattacaa cggtaaagat atgatattaa
     3661 atgaaaaggt aaattcggga aatattttaa tagatggtat tggtattggg gatgtaggaa
     3721 atatcgtgtt gagagaccgt catcttttag cagaagatgg tatctttatt gctgtagtaa
     3781 cgttagatcc taaaaataga cgtatagctg cgggtcctga aattcaatct cgtgggtttg
     3841 tatatgtacg tgaaagtgaa gacttattac gtgaagcaga agagaaagta cgtgaaatag
     3901 tagaagctgg tttacaagaa aaacgcatag aatggtctga aattaagcaa aatatgcgtg
     3961 atcaaattag taaactatta ttcgaaagta caaaacgtcg tcctatgatt attccagtaa
     4021 tttctgaaat ttaatcaaaa agtcattaac ataaaagagg tcagaacaag tcaccgaaat
     4081 ataatggttg tcatggacaa tttacttata ttttatgatg gtcaattgaa aggggtaacg
     4141 attaatctgt tatcttaagt aaattgatac atagatgata ttgttctaac ctctttcatc
     4201 gtctgtttgg actacatatt ctaaacatca aataggaaat tatatataat aacgtcgttt
     4261 taactaaggc aacataagga ggtgcgtcaa ttggcacaag caaaaaagaa atcgacagct
     4321 aagaaaaaaa cagcatcaaa aaaaagaaca aattcaagga aaaagaagaa tgataatccg
     4381 atacgttatg tcatagctat tttagtagtt gtattaatgg tgttgggtgt tttccaatta
     4441 ggaataatcg gtcgtctaat tgacagcttc tttaattatt tatttgggta cagtagatat
     4501 ttaacatata ttttagtact cttagcaact ggttttatta catactctaa acgtattcct
     4561 aaaactagac gaacggctgg ttcgattgta ttgcaaattg cattgctatt tgtatcacag
     4621 ttagtttttc attttaatag tggtatcaaa gctgaaagag aacctgtact ttcttatgta
     4681 tatcagtcat accaacacag tcattttcca aattttggtg gcggtgtatt aggtttttat
     4741 ttattagagt taagcgtacc tttaatttca ttatttggtg tatgtattat tactatttta
     4801 ttattatgct caagtgttat tttattaaca aaccatcaac atcgtgatgt tgcaaaagtt
     4861 gcactggaaa atataaaagc ttggtttggt tcatttaatg aaaaaatgtc ggaaagaaac
     4921 caagaaaaac aattgaagcg tgaagaaaaa gcgagactta aagaagaaca aaaggcacgt
     4981 caaaatgaac agccacaaat aaaagatgtg agtgatttta cggaagtgcc tcaagaaaga
     5041 gatattccaa tttatgggca tactgaaaat gaaagtaaaa gccagtgtca accaagtcga
     5101 aaaaaacgag tgtttgatgc agagaatagt tcgaataaca tcgtaaatca tcaagcagat
     5161 cagcaagagc aattaacaga acaaactcat aacagtgttg aaagtgaaaa cactattgaa
     5221 gaagctggtg aagttacgaa tgtatcgtat gttgttccac cgttaacttt acttaatcaa
     5281 cctgcaaaac aaaaagcaac atctaaagct gaagtacaac gtaaaggaca agtactagag
     5341 aatacattaa aagattttgg ggtaaatgca aaagtgacac aaattaaaat tggtcctgca
     5401 gtaactcaat atgaaattca accagctcaa ggggttaaag tgagtaaaat tgtaaacttg
     5461 cataatgata ttgcattagc tttagcagca aaagatgtta gaatcgaagc accaatacct
     5521 ggtcgctctg cagtaggtat tgaagtgcca aatgagaaaa tttcattagt ttcactaaaa
     5581 gaagttttag atgaaaaatt cccgtctaat aataaactag aagttggatt aggaagagat
     5641 atatcaggtg atccaattac tgttccacta aatgaaatgc cacacttatt ggtggcagga
     5701 tcgacgggta gtggtaaatc tgtttgtata aatggtatta ttacaagtat tttattaaat
     5761 gctaagccgc atgaagttaa acttatgtta atcgatccga aaatggttga actaaatgtt
     5821 tataacggaa ttccacattt attaattccg gttgttacaa atcctcataa agctgctcaa
     5881 gctttagaaa aaattgtagc tgagatggaa agacgttatg atttattcca acattcatca
     5941 actagaaata ttaaaggtta taacgaatta atccgtaagc aaaatcaaga attagatgag
     6001 aagcaaccag aattacctta tatcgttgtt attgtagatg agcttgcaga tttaatgatg
     6061 gtagctggta aagaagttga aaatgcgatt caacgtatca cacaaatggc acgtgcagca
     6121 ggtatacatt tgattgtagc aacacaaaga ccttctgtgg atgtaattac aggtatcatt
     6181 aaaaataaca ttccatctag aattgctttt gctgtgagtt ctcaaacaga ttcaagaact
     6241 attattggta ctggcggtgc agaaaagtta cttggtaaag gtgacatgtt atacgttgga
     6301 aatggtgatt catcacaaac acgtattcaa ggggcgtttt taagtgacca agaggtgcaa
     6361 gatgttgtaa attatgtagt agaacaacaa caggcaaatt atgtaaaaga aatggaacca
     6421 gatgcaccag tggataaatc ggaaatgaaa agtgaagatg ctttatatga tgaagcgtat
     6481 ttgtttgttg ttgaacaaca aaaggcaagt acatcattgt tacaacgcca atttagaatt
     6541 ggttataata gagcatctag gttgatggat gatttagaac gcaatcaggt aatcggtcca
     6601 caaaaaggaa gcaagcctag acaagtttta atagatctta ataatgacga ggtgtaaaaa
     6661 aatgtcagaa atgaatgcgg tatataacgt taaacaatac attttaaatt tgattatgca
     6721 aaataaattg gaatatggtg accaacttcc aagtaattta tcaattgcca gagaattaaa
     6781 tgtaaaaacc gacgatgttt atgaagcaat tcaggcattg attactgaac aagtcattaa
     6841 agataatttt gaagagggca caagtgttaa atcactgccc cctttctttt atccattgaa
     6901 tgaacttata agtattgggc aaatgattaa aaatgcagga tttgaatgcg gaactgaata
     6961 cttaaatttt gatgagcaac cagcaactat gttagatgca aatttgttga gcgttgaaga
     7021 aggatatcca gtaaccatta tagaacgatt acgaactgcc gatggagaac cggtcgtcta
     7081 ttgtttagat aaaattgcta aaaaagaatt aacatgtaca gagtatcaaa tgagcaatgg
     7141 atcgatacta agtgcgataa aagaacaaag taatcataat atttgttacg ctgatacaga
     7201 aattgaagcg gtaaattatg aacctcgaat atccgaagta ctgaatgctt cgccacacga
     7261 aggtttgatt ttattaaaaa ttacgcacta caatgaatta gatgaaccta ttttgtattc
     7321 attaaattat atgaaaaata gcttagttca atttaaaatc actagaaaaa tatagatgta
     7381 agaagatata gaggaggcta tatatttgag tagacaatct caaccaaata tacatatcaa
     7441 agtttcacca acaactaaat ttaaaacaac tactatagtt tttaaattta tggcaccttt
     7501 agaatatgac acaattacag ctagatcatt attaagcaaa ttattagttc gagcaactaa
     7561 gaaatggcca accgataagt cgtttaataa tcacttagcc gatttatatg gtgcgtatgt
     7621 gaatagtaca atttcaaaat tcaaagatca gcatgtcatt acattttcat tagaaattgt
     7681 taatgaacgt tatttaagaa acggtgaatc attatttaat caaggattag atttattaca
     7741 agaaatcatt tggaatccat taattgaaaa taaagcattc aatgataatt ttgttaacca
     7801 agagaaaaca ttattagcca aaaaaataga agcaatggta gataataaag cacaatattc
     7861 gtttttaaaa ttactcgacc atatgtttga aaatgaagca tataaatact tatctacagg
     7921 acaactagaa caaatcccac atattactgc tgaaacacta tatcatacat atcaatcaat
     7981 gattaataat gatcaatgtt ctgtttatgt tgtcggcaat gtagaacctg aaagtgttga
     8041 gaaacaaata cgtgaaaaat ttgcacttaa accattcgat aaacatcaat tccaacattc
     8101 tactcatcat ttacacgatg aagaagttga ttatattgtt gaatatgatg acgtggatca
     8161 agctaaatta aatatgggat accgttttcc aacacaatat ggacaaagtg gatatgctgc
     8221 gtttgttgta tttaacatga tgtttggagg agatccttca tctgttttat ttaatgaagt
     8281 gcgagaaaag caaagtttag cgtactctat acattcacaa attgatggca aaaatggcta
     8341 tttatttgtt ttgagtgggg tttcaagtga taagtacgaa actgcaaaag acactattat
     8401 aagtgaattt gaaaaaataa aagcaggaga tttcactgaa gaaaaattag agttagctaa
     8461 aaaagtaatc atttctcatc gatatgaatc tgaagatcgt ccgaaaagta ttatagagat
     8521 tatgcataac caaatattat tagagcaacc acaaagcaaa gaaacattta taaatgatat
     8581 acagaaggta agtcgcgaag atattgtttc tgttgctgaa aaagcatttt tagatacaat
     8641 ctatgtgttg acaaaaggag gggataaata atgaaagagc gttattatga attaatagac
     8701 gaaagagtat tcgaacaaga attagaaaat ggtttacgat tatttattat ccccaaacca
     8761 ggttttcaaa agacatttgt cacttacact acacaatttg gttcattaga taatcaattc
     8821 aaaccccttg gacaagacca atttgttact gtacctgatg gagttgctca ctttttagaa
     8881 cataaattat ttgaaaaaga agaagaagac ttatttactg cgtttgctga agataatgca
     8941 caagcaaatg cgtttacaag ctttgatcgt acaagctact tgttcagtgc aactgataat
     9001 attgaaaaca acattaaacg tttacttaca atggttgaaa cgccttattt tacaaaagaa
     9061 actgttgata aagaaaaagg tattattgca gaagaaataa aaatgtatca agaacaacct
     9121 ggatataaat taatgtttaa tacattgcgt gcaatgtatc aacgacatcc aatacgtgtt
     9181 gatattgccg gtagtgtaga aagtatatac gatattacaa aagatgattt gtatctatgt
     9241 tatgaaacgt tttatcatcc atcaaatatg gttttatttg ttgttggtga tgtgaatcca
     9301 gaagaaatat gtcgaatagt aaaacaacac gaggatgctc gtaataaagt taaccaaccc
     9361 aaaatcgaac gaggacttgt tgatgaaccg gaggatgtta aagaagcatt tgttactgaa
     9421 tctatgaaaa ttcaatcacc aagactaatg cttggtttta aaaataaacc attacaagaa
     9481 gcgcctcaaa aatatgtaca acgtgattta gaaatgtcat tattctttga gttaattttt
     9541 ggggaagaaa cagattttta tcagaattta ttaaacgaag gacttatcga tgatacattt
     9601 ggttatcaat ttgtactaga gccgacgtat agtttttcaa tcgtgacaag cgctactgaa
     9661 gaaccagata aattaaaaaa attattatta gatgagttgc gtgataaaaa aggcaatttc
     9721 caagatgcag aagcatttga acttttgaaa aagcaattta taggtgaatt catatcaagt
     9781 ttaaactcac ctgaatatat tgctaatcaa tatactaaat tgtattttga aggtgttagc
     9841 gtgtttgaca tgttagacat tgttgaaaat atcacgttag atagtattaa cgaaacgtca
     9901 tcgttatatt taaatttaga tcagcaagtc gatagtcgct tggagattaa aaagtaatga
     9961 aagcattagt attaggtggt tctggttcaa ttggttctga gatagtcaaa caattattaa
    10021 ctgatggatt tgaagtttat gtgcaatatt atcgtactga tataaatgaa ttaactagca
    10081 aatttaatga tgataaagtt cgttttatac aagcggattt atctcaaaca attgatattg
    10141 acaaaacatt tggtgacatt aaatcattag actgtttaat atatgcaagt ggtcagtctt
    10201 tatatggtgt tttacaagat atgaaagacc atgatattga tgcatgttat cagttaaatg
    10261 tcttgcaatt aattcgatta tgtagatatt tcgttgatgt tttacgtcaa agtgccaatg
    10321 gaagaattat tgtaatttca tcaatttggg gtgagacagg agctagtatg gaaactattt
    10381 attcggcgat gaaaagtgca caattaggtt tcgttaaggc gcttagtcaa gagcttgcac
    10441 taacatcagt gacagtaaat gctatcgcac ctggatttgt agccggtaat atggcaagtg
    10501 agtggcaaga agatgaactt caagcaatga taactgaatt accacaacag cgattggttt
    10561 taccgagtga ggttgctcat acatgcgcct atttatatca ctcaaatgct agaagcgtta
    10621 ctggaactat acagaaagtt aatggtgctt ggtatattta aaatatagaa ctatttttat
    10681 gagcaaatgt ttttatgatt gatgttataa tatgaatatt acaatgaatc ataatttgtt
    10741 aattaaacaa ggggtgaggt tcaccatgac agttgcagag aaaaaagaat ggtacctaga
    10801 atacgaaatt gaaattaata gaccgggtct tttaggtgat gtatctagtt tattaggtat
    10861 gttaggtata agtattgtta caattaatgg tgttgatcaa ggtaaacgag gccttttaat
    10921 taaaacagac aatcttgaaa aagttgaacg ttttgagcaa atagctcgtg gtataaatga
    10981 aattgaaata acaaagctta aaaaaccaga attaagagac cgtcttgcag ttagacatgg
    11041 tagatatatt gagcaagatg caaaagataa gaaaaccttt cgatttgagc gtgaagattt
    11101 aggcttgtta gtagactttt tagctgaatt gttcaaagaa gaaggtcata agttgattgg
    11161 cattagaggt atgccacggg ttggtaaaac tgaatcaatt gttgcgggaa gtgtttgtgc
    11221 acataagaga tggttattta ttagttctac tttaataaaa caaactgtac gtagctctct
    11281 aattaaaggg gaatatgatg ccaatcatgt atacattatt gatggtgcag ttactgccag
    11341 agaatctaat ccaaaacatc aagagcttgt taacgaagtt atgacgttac catcaatcaa
    11401 agtcgttgaa catccagatt tatttgttga aacaagtact tgtacaatgg aagattttga
    11461 ctatattatc gaattgagag aaaatgaaaa tcaagaaata cattacgaag aaatgaagaa
    11521 acagacagtc caaagtaaga ataatttaga ttttggagat ccgtttggtg gtggttttgg
    11581 tttcttcgag taagtgtaag gaggctatga attgaaaacg gtcggtgaag cgctaaaagg
    11641 tagacgtgaa aggttaggaa tgactttaac agaattagag caacgtactg gaattaaacg
    11701 tgaaatgcta gtgcatattg aaaataatga attcgatcaa ctaccgaata aaaattacag
    11761 cgaaggattt attagaaaat atgcaagcgt agtaaatatt gaacctaacc aattaattca
    11821 agctcatcaa gatgaaattc catcgaacca agccgaatgg gacgaagtaa ttacagtttt
    11881 caataataat aaagacttag attataagag taaatcaaaa gagccaatac aattattagt
    11941 aatcatgggt attacagttt taataacttt attgttatgg atcatgttag ttttaatatt
    12001 ttaacagaaa taaattagtg agaaatgagg atgtataatg aatattccga accagattac
    12061 ggtttttaga gtagtgttaa taccagtttt tatattgttt gcgttagttg attttggatt
    12121 tggcaatgtg tcatttctag gaggatatga aataagaatt gagttattaa tcagtggttt
    12181 tatttttata ttggcttccc ttagcgattt tgttgatggt tatttagcta gaaaatggaa
    12241 tttagttaca aatatgggga aatttttgga tccattagcg gataaattat tagttgcaag
    12301 tgctttaatt gtacttgtgc aactaggact aacaaattct gtagtagcaa tcattattat
    12361 tgccagagaa tttgccgtaa ctggtttacg tttactacaa attgaacaag gatttgtaag
    12421 tgcagctggt caattaggta aaattaaaac agcagttact atggtagcaa ttacttggtt
    12481 gttattaggt gatccattgg caacattgat tggtttgtca ttaggacaaa ttttattata
    12541 cattggcgtt atttttacta tcttatctgg tattgaatac ttttataaag gtagagatgt
    12601 ttttaaacaa aaataaatat ttgtttatac tagatttcat tttcatatgg aatctagttt
    12661 ttttaatccc aattttagaa attagccacg caattgttta taatgatata ttgtaaaaca
    12721 atatttgttc attttttagg caaatgtgtt gtagcatctg atacattgaa tctaaaattg
    12781 atgtgaattt ttaaatggaa tacatgaaaa aagaattaaa cgatacaagg gggatataaa
    12841 tgtcaattgc cattattgct gtaggctcag aactattgct aggtcaaatc gctaatacca
    12901 acggacaatt tctatctaaa gtatttaatg aaattggaca aaatgtatta gaacataaag
    12961 ttattggaga taataaaaaa cgtttagaat caagtgtacg tcatgcgtta gaaaaatatg
    13021 atactgttat tttaacaggt ggcttaggtc ctacgaaaga tgacttaacg aagcatacag
    13081 tggcccagat tgttggtaaa gatttagtta ttgatgagcc ttctttaaaa tatattgaaa
    13141 gctattttga ggaacaagga caagaaatga cacctaataa taaacaacag gctttagtaa
    13201 ttgaaggttc aactgtatta gcaaatcatc atggcatggc tccaggaatg atggtgaatt
    13261 ttgaaaacaa acaaattatt ttattaccag gtccaccgaa agaaatgcaa ccaatggtga
    13321 aaaatgaatt gttgtcacat tttataaacc ataatcgaat tatacattct gaactattaa
    13381 gatttgcggg aataggtgaa tctaaagtag aaacaatatt aatagatctt atcgataaac
    13441 agactaatcc tacgattgcg cctttggcgg gaagtcatga agtatatatt agattgactg
    13501 caaatgccga ctcaaaagaa caagcacaat cattgattca acctgttaaa caagaaattc
    13561 ttgatcgtat tggagaatat tattatggtt cagatgacac attaattgag caagctgtaa
    13621 taaagaaaat tcatgaacct tttgtaatat atgatggtat tactaatggt gctttatatc
    13681 atcgattgaa agaagtggat ttaaacgatg ttctaaaggg tatgattaat cacaatgaaa
    13741 actttgttga tattaataaa cctattgagc agcaattaaa agatgcagtg caatttgtta
    13801 ataaattgtt taatgtgtca tcagcaatta ttctattaga gtatgatggt gtagtccata
    13861 taggctatga taataacttt gaatttaaaa ctgagcaatt taaaatgtct aaatctagaa
    13921 atttattaaa gaacagaagt caaaattatg tgctcataag attattaaat tggcttagaa
    13981 caacaaatta attgtattat cgataaaaat ataagcacgt ttgttcgttt ttgcgttttg
    14041 atttcaagat tttatacgaa caaatattcg caaaacactt gtattttatt ttgaatcctt
    14101 gtatagtatt gataagataa ttaaaagata gcaatttcaa ttaggaggtc tcgctttgga
    14161 taacgatcgt caaaaagctt tagatacagt aattaaaaat atggagaaat ctttcggtaa
    14221 aggtgccgta atgaagttgg gtgacaatat aggtcgccga gtttcaacta catcaactgg
    14281 ttcagttaca ttagataatg cgctaggtgt aggtggctat cctaaaggac gaattattga
    14341 aatttatggt cctgaaagtt ctggtaagac aacagtagcg cttcacgcta ttgctgaagt
    14401 acaaagtaat ggcggggtgg cagcatttat cgatgctgaa catgctttag atccagaata
    14461 tgctcaagca ttaggcgtag atatcgataa tttatattta tcgcaaccgg atcatggtga
    14521 acaaggtctt gaaatcgccg aagcatttgt tagaagtggt gcagttgata ttgtagttgt
    14581 agactcagtt gctgctttaa cacctaaagc tgaaattgaa ggagaaatgg gagacactca
    14641 cgttggttta caagctcgtt taatgtcaca agcgttacgt aaactttcag gtgctatttc
    14701 taaatcaaat acaactgcta ttttcatcaa ccaaattcgt gaaaaagttg gtgttatgtt
    14761 cggtaatcca gagactacac caggtggacg tgcattaaaa ttctatagtt cagtaagact
    14821 agaagtacgt cgtgcagaac agcttaaaca aggacaagaa attgtaggta atagaactaa
    14881 aattaaagtc gttaaaaata aagtggcacc accatttaga gtagctgaag ttgatattat
    14941 gtatggacaa ggtatttcta aagagggtga acttattgat ttaggtgttg aaaacgacat
    15001 cgttgataaa tcaggagcat ggtattctta caatggcgaa cgaatgggtc aaggtaagga
    15061 aaatgttaaa atgtacttga aagaaaatcc acaaattaaa gaagaaattg atcgtaaatt
    15121 gagagaaaaa ttaggtatat ctgatggtga tgttgaagaa acagaagatg caccaaagtc
    15181 attatttgac gaagaatagt acacaaattt atatctatag ttaaacttag caaatatcct
    15241 tataggattg attgaaagtg atattcatct cataaagcta gaataatatc taactttatg
    15301 ggatacacta caaatcgaga cta
//
LOCUS       DANBWH010000046        15132 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.048, whole genome shotgun sequence.
ACCESSION   DANBWH010000046 DANBWH010000000
VERSION     DANBWH010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15132
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.048"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            158..874
                     /locus_tag="PPY92_000987"
     CDS             158..874
                     /locus_tag="PPY92_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDJ2625005.1"
                     /translation="MGRKWNNIKEKKAQKDKNTSRIYAKFGKEIYVAAKSGEPNPESN
                     QALRLVLERAKTYSVPNHIIEKAIDKAKGAGDENFDHLRYEGFGPSGSMLIVDALTNN
                     VNRTASDVRAAFGKNGGNMGVSGSVAYMFDHVATFGIEGKSVDEILETLMEQDVDVND
                     VIDDNGLTIVYAEPDQFAVVQDALRAAGVEEFKVAEFEMLPQTDIELSEADQVTFEKL
                     IDALEDLEDVQNVFHNVDLK"
     gene            874..1347
                     /locus_tag="PPY92_000988"
     CDS             874..1347
                     /locus_tag="PPY92_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDJ2625006.1"
                     /translation="MKSAEQWIDELQLESHPEGGFYRETIREVLKDGRRAPFSSIYFL
                     LTDDNISHFHRIDADEVWYYHAGDSLTIHMINPDGEYTTATLGTDIQNGDVLQYVVPK
                     GTIFASSIEISNTYSLVGCMCQPAFEFKQFELFKQSELITQYPHLKSVIEKYALK"
     gene            1439..1555
                     /locus_tag="PPY92_000989"
     CDS             1439..1555
                     /locus_tag="PPY92_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625007.1"
                     /translation="MLQLFLMKIALNDFENDKSVLFIFLKNHFYEEMCVLSN"
     gene            1700..2344
                     /locus_tag="PPY92_000990"
     CDS             1700..2344
                     /locus_tag="PPY92_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDJ2625008.1"
                     /translation="MKVKYIDKRHWRRLIDREYTEVKVNNNRFKGIIGLVTMKKVRDP
                     LEVTVVGQNIIVADDNYKWLQILPEKKRYSITVMFDNKGNPLEYYFDINIKNITQKGN
                     ARTVDLCLDVLALPSGEYELVDEDDLMFALESEQITKKQFHEAYMIAHQIMAELENDF
                     KGFQKKIMYCFNKINAKAQKNHQKPQNKTNIEKSKQIKPKQYNQTKNHQQQKKN"
     gene            2460..3326
                     /locus_tag="PPY92_000991"
     CDS             2460..3326
                     /locus_tag="PPY92_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDJ2625009.1"
                     /translation="MKIEDYRLLITLDETKTLRKAAEILYISQPAVTQRLKAIENAFG
                     VDIFIRTKKQLITTTEGTMIIEHARDMLKRERLFFDKMQAHIGEVNGTISIGCSSLIG
                     QTLLPEVLSLYNAQFPNVEIQVQVGSTEQIKANHRDYHVMITRGNKVMNLANTHLFND
                     DHYFIFPKNRRDDVTKLPFIEFQADPIYINQIKQWYNDNLEQDYHATITVDQVATCKE
                     MLISGVGVTILPEIMMKNISKEQFEFEKVEIDNEPLIRSTFMSYDPSMLQLPQVDSFV
                     NLMASFVEQPKA"
     gene            3458..4678
                     /locus_tag="PPY92_000992"
     CDS             3458..4678
                     /locus_tag="PPY92_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HDJ2625010.1"
                     /translation="MDMFAALLQIKNYKLFVANMFLLGMGIAVTVPYLVLFATKDLGM
                     TTNQYGLLLASAAISQFTVNSIIARFSDTHHFNRKIIIILALLMGALGFSIYFFVDTI
                     WLFILLYAIFQGLFAPAMPQLYASARESINVSSSKDRAQFANTVLRSMFSLGFLFGPF
                     IGAQLIGLKGYAGLFGGTISIILFTLVLQVFFYKDLNIKHPISTQQHVEKIAPNMFKD
                     KTLLLPFIAFILLHIGQWMYTMNMPLFVTDYLKENEQHVGYLASLCAGLEVPFMIILG
                     VLSSRLHTRTLLIYGAIFGGLFYFSIGVFKNFYMMLAGQVFLAIFLAVLLGIGISYFQ
                     DILPDFPGYASTLFSNAMVIGQLGGNLLGGAMSHWVGLENVFFVSAASIMLGMILIFF
                     TKNQKITKEDMIST"
     gene            4675..5163
                     /locus_tag="PPY92_000993"
     CDS             4675..5163
                     /locus_tag="PPY92_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HDJ2625011.1"
                     /translation="MTIILWLLIIAAFMLAFVGLIKPIIPSVLVLWVGFLIYQFGFHN
                     QHLSWVFYVSMALLTILILCADFLANKYFVNRFGGSKFGEYAALIGVVIGCFVLPPFG
                     IIIIPFILVFIVELIQGYSFERAVKVSIGSIVAFLTSSIAQAIIMFIMIVWFFIDALL
                     IN"
     gene            complement(5262..5951)
                     /locus_tag="PPY92_000994"
     CDS             complement(5262..5951)
                     /locus_tag="PPY92_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HDJ2625012.1"
                     /translation="MKSTAQLTKENNVKSLRLSNTDREIFENYMTYMRSDFRVNPHDT
                     ELIINRILKQLLSAEQHGLLALDFFNHDPKAHAIKELKAMPNETFKNIFKYIYQHIVL
                     LIGIVSFLKGFLGFFMEKNGSNLYFVSFPFSVVVGFFIVFLFIWFSFKTIQLQCFNNS
                     NWIWIFTYLAIILLIVGFFYVFFIPQSLLAFGPYIQVSNWVFIIFSFIVMPIGLRIER
                     NISKKHSHTFL"
     gene            6078..6521
                     /locus_tag="PPY92_000995"
     CDS             6078..6521
                     /locus_tag="PPY92_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDJ2625013.1"
                     /translation="MRTLNKDEHNYIKQIANIHETLLSQAESNYKCTKLSIALRYEMI
                     CSRLEHTNDKIYIYENEGQLIAFIWGHFSNEKSMVNIELLYVEPQFRKLGIATQLKIA
                     LEKWAKTMNAKRISSTIHKNNLPMISLNKDLGYQVSHVKMYKDID"
     gene            6588..6983
                     /locus_tag="PPY92_000996"
     CDS             6588..6983
                     /locus_tag="PPY92_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625014.1"
                     /translation="MKKLIISIMAVMLFLTGCGKSQEKATLEKDIDNLQKENKELKDK
                     KEKLQQEKEKLADKQKDLEKEVKDLKPSKEDNKDDKKDEDKNKDKDKDKEASQDKQSK
                     DQTKSSDKDNHKKPTSTDKDQKANDKHQS"
     gene            7121..7420
                     /locus_tag="PPY92_000997"
     CDS             7121..7420
                     /locus_tag="PPY92_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625015.1"
                     /translation="MHEQDFRILEGQDITLPELGRELENITGHTIADSTGEIKRVIAH
                     LPNFESDTDTFVATYRLNHQQDFIDATFTALKSDRARLKEVPVHVELISYISKSK"
     gene            7500..8042
                     /locus_tag="PPY92_000998"
     CDS             7500..8042
                     /locus_tag="PPY92_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDJ2625016.1"
                     /translation="MIYCETERLILRDWHEDDLLPFQKMNANYDVRKYFPSLLSYRRS
                     ELDMRTMDAVIKDYGIGLFAVEDKESHQWIGFIGLNYIPETSDYPFKELPLYEIGWRL
                     LPEFWGKGLATEGAKATLKLAEEHQIYDVYSFTAEANKASQRVMEKIGMTVYDHFELP
                     NLSKYHLLKRQVRYYINLRK"
     gene            complement(8138..8704)
                     /locus_tag="PPY92_000999"
     CDS             complement(8138..8704)
                     /locus_tag="PPY92_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HDJ2625017.1"
                     /translation="MKRIAVYCGASKGHDPSYVQKAYDLGKYFAEQGYELVFGAGSIG
                     IMGAIQDGVLNHGGKAIGVMPKMLDEHEITSQRLTELILVDSMHERKNKMAELADAFV
                     MAPGGAGSLEEFFEMYSWAQIGIHEKPIAIYNINGFFNPLQTMIDHMIEEGFIDPKYR
                     ALAPLCDTKESLIESILNFKPLGTRSYD"
     gene            complement(8706..9164)
                     /locus_tag="PPY92_001000"
     CDS             complement(8706..9164)
                     /locus_tag="PPY92_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDJ2625018.1"
                     /translation="MTHIIIDGDACPVVDSIIDLTTETGIFVTIIRSFSHFSNQLYPP
                     HVSTLYVDDGPDAVDYKIVQLSTKDDIVVTQDYGLASLLVDKVLIVMHHNGKIYNSKN
                     IQQLLDKRYINAQIRKQGGRHKGPPPFTKQDQKVFEQSLLKVIHRIKELD"
     gene            complement(9167..9850)
                     /locus_tag="PPY92_001001"
     CDS             complement(9167..9850)
                     /locus_tag="PPY92_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625019.1"
                     /translation="MDKKKVIKFMINVLPIVLVPLIVERKRIKQHPDVQKVTDATSKV
                     ASKTSAAISNTASDVKEYVGDKKQDFENKRELKKFAREHDPAYIEKKGEKLAKQNRKD
                     ADKMNKILQKNIEKRHKEEQKAREKNEIQRIKDMKKSQKYEEKAGLTPNKLDEKTEKK
                     GDKLAEKNRKEIAKMNKKLQKNIEKRHKEEQKRQQEADKARIKSFKKYKDYVAKSASQ
                     QNKENNTEA"
     gene            complement(10022..10897)
                     /locus_tag="PPY92_001002"
     CDS             complement(10022..10897)
                     /locus_tag="PPY92_001002"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDJ2625020.1"
                     /translation="MFIIELIKGIILGVVEGLTEFAPVSSTGHMILVDDMWLKSSEFL
                     GSQSAFTFKIVIQLGSVFAAAWVFRERFLEILHIGKHKHVEGENDQQRRSKPRRLNLL
                     HVLVGMVPAGILGLLFDDFIEEHLFSVPTVMIGLFVGAIYMIIADKYSVKVKNPQTVD
                     QINYFQAFVIGISQAVAMWPGFSRSGSTISTGVLMKLNHKAASDFTFIMAVPIMLAAS
                     GLSLLKHYQDIQIADIPFYILGFLAAFTVGLIAIKTFLHLINKIKLIPFAIYRIVLVI
                     FIAILYFGFGIGKGI"
     gene            11116..12747
                     /locus_tag="PPY92_001003"
     CDS             11116..12747
                     /locus_tag="PPY92_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HDJ2625021.1"
                     /translation="MKKLTTILFQYKIFPVLMFLVSTGLGILVITQNILIADFLAKII
                     RHQFQGLWIVLFILLGVLLLRATVQFLNQWLGDTLAFKVKHMLRQLVIYKNNGHPIGE
                     QMTILTENIDGLAPFYKSYLPQVFKSMMVPLIIIIAMFFIHFNTALIMLITAPFIPLF
                     YIIFGLKTRDESKDQMTYLNQFSQRFLNIAKGLVTLKLFNRTEQTEKHIYDDSTQFRT
                     LTMRILRSAFLSGLMLEFISMLGIGLVALEATLSLVVFHNIDFKTAAIAIILAPEFYN
                     AIKDLGQAFHTGKQSEGASDVVFEFLEQPNYNNEFLLKYEENQKPFIQLTDISFRYDD
                     SDRLVLNDLNLEIFKGDQIALVGPSGAGKSTLTHLIAGVYQPTIGTISTNQRDLNIGI
                     LSQQPYIFSASIKENITMFKDIENNTIEEVLDEVGLLDKVQSFTKGINTIIGEGGEML
                     SGGQMRRIELCRLLVMKPDLVIFDEPATGLDIQTEHMIQNVLFQHFKDTTMIVIAHRD
                     NTIRHLQRRLYIENGRLIADDRNISVNITENGDDL"
     gene            12744..14417
                     /locus_tag="PPY92_001004"
     CDS             12744..14417
                     /locus_tag="PPY92_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding/permease
                     protein"
                     /protein_id="HDJ2625022.1"
                     /translation="MKTRLKFQVDKDLLLAIVVGVCGSLVALAMFFLSGYMVTQSALG
                     APLYALMILVVTVKLFGFLRAITRYVERLISHKATFTMLRDIRVQFFGKLVNVIPNVY
                     RKLSSSDLISRMISRVEALQNIYLRVYYPPVVIGLTALVTVIVLAFISIGHALLIMVS
                     MLFTLLIVPWLSSKKARTLKKHAANEQARFLNHFYDYKAGMDELRRFNQINHYRDNLM
                     AKLNHFDKLQLKEQRFLTIYDFILNIIAMLSIFGSLVLGLIQINAGQLNIIYMTSIVL
                     MILTLFEQAVPMTNVAYYKADTDQALHDINEVISVPSTNGKKRLNDKYDATNIYEVKD
                     ASFKYWNQQTYVLSDINFNVNRGEKIAIVGPSGSGKSTLLQIMAGLYQLDSGSVRFEN
                     MDMFEIDDKDKFESLNVLLQSQQLFDGTIRQNLFTDEKDEAVQAIFKQLDLEHLALER
                     QIDLDGHTLSGGEIQRLAITRMLLKDTASTWILDEPTTALDKQNSLKVMDLIEAHAET
                     LIVATHDLTLLSRFETIIVMINGKIVEKGNYQQLLANQGALWNMIQYNA"
     gene            complement(14544..14987)
                     /gene="mgrA"
                     /locus_tag="PPY92_001005"
     CDS             complement(14544..14987)
                     /gene="mgrA"
                     /locus_tag="PPY92_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MgrA"
                     /protein_id="HDJ2625023.1"
                     /translation="MSDQHNLKEQLCFSLYNAQRQVNRYYSNKVFKKYNLTYPQFLVL
                     TILWDESPVNVKKVVTELALDTGTVSPLLKRMEQVDLIKRERSEVDQREVFIHLTDKS
                     ETIRPELSNASDKVASASSLSQDEVKELNRLLGKVIHAFDETKEK"
BASE COUNT         5215 a         2130 c         2623 g         5164 t
ORIGIN      
        1 ttatcggcac atttttaatt tatacatatt ttaaaactaa gtaacagttt gaaggaatcg
       61 tagttcaata atgttaattg tgaaaatgta tataaacata aaaaaatcat gtataatata
      121 tgttgttaat taaacagttc gaaagcgaga tgacattatg ggacgtaaat ggaataacat
      181 taaagaaaaa aaggcccaaa aagataaaaa cacaagtaga atatatgcga aatttggtaa
      241 ggagatttat gttgcagcaa aatctggtga acccaatcca gaatctaacc aagctttaag
      301 gttggtgctt gaacgcgcta agacatattc agtgccgaat catattattg aaaaagcaat
      361 agataaagct aagggtgctg gagacgaaaa ctttgatcac ctaagatatg aaggatttgg
      421 cccaagcgga tcaatgctaa ttgttgatgc gttaacaaat aatgtaaatc gtactgcctc
      481 tgatgtgcga gctgcttttg gtaaaaacgg cggtaatatg ggtgtatctg gatcagttgc
      541 ttatatgttt gatcatgtgg caacatttgg tattgaagga aagtctgttg acgaaatact
      601 tgaaacatta atggaacaag atgtagatgt aaatgatgtg attgacgata atggattgac
      661 aatagtctat gctgaaccag atcaatttgc agtcgttcaa gatgcgcttc gtgcagcagg
      721 tgttgaagaa tttaaagttg ctgaatttga aatgttacct caaacagata ttgaactttc
      781 tgaagcggac caagtaacat ttgaaaaatt aatcgatgca ttagaagatt tagaagatgt
      841 acaaaacgta ttccataatg tggatttgaa ataatgaaat cagcagaaca atggattgat
      901 gaattgcaac ttgaatcaca tcctgaaggt ggtttctata gagagacaat tcgagaagta
      961 ttgaaagatg gacgcagagc gccgtttagt agtatttatt ttttacttac agatgacaat
     1021 atttcgcatt ttcatcgaat tgatgctgat gaagtatggt actatcatgc tggtgattct
     1081 ctaacaattc atatgataaa tccggatggg gaatatacga ctgcaacatt gggtactgat
     1141 atccaaaatg gagatgtatt gcaatatgta gtgcctaaag gaacaatttt tgcttcttca
     1201 atcgaaattt caaatactta tagtttagta ggttgtatgt gtcaaccggc atttgagttt
     1261 aagcagtttg aattgtttaa gcaatctgaa ttaattacac aatatccgca tcttaaatca
     1321 gtgattgaaa aatatgcttt aaaataaaaa tgatcaatga agtggtttga aggttgttaa
     1381 taaacctttg agtcacttca tttttatatg tattcttgat tgaatcagaa tagatttgat
     1441 gcttcagctg tttttaatga aaatagcatt aaatgatttt gaaaacgata agagtgtgtt
     1501 atttatattt ttgaaaaatc acttttatga agaaatgtgt gtactatcta attaatattg
     1561 tctattttaa gtaaattgaa gcgagttgta gtaaaataat aataaatatt ttcatatgat
     1621 taacaaaaac tataaaactg tatcatgaca tgacatcatt tatgtagttt tatataataa
     1681 tgacaaggag ttggtggctg tgaaagtaaa gtatatagat aaacgtcact ggcgtcgcct
     1741 aattgatagg gaatacacag aggtaaaagt taataataat aggtttaagg gtattatagg
     1801 cttagtcacg atgaaaaagg ttcgtgatcc tttagaggtg acggtagttg gacaaaatat
     1861 cattgtcgca gatgacaatt ataaatggtt gcaaatacta cctgaaaaga aacgttatag
     1921 tataactgta atgtttgata ataaaggcaa tccattagaa tattattttg atataaatat
     1981 caaaaatata acgcaaaaag gtaatgcgcg tacagtagat ttatgtttag atgttttagc
     2041 attaccaagt ggtgaatatg agttggtaga tgaagatgat ttaatgtttg cattagaaag
     2101 tgagcaaatt acaaaaaagc aatttcatga agcatatatg attgcacatc aaattatggc
     2161 agagttagaa aatgatttta aaggattcca aaagaaaatc atgtactgct ttaataaaat
     2221 taatgcaaag gctcaaaaaa atcatcaaaa gccacaaaat aaaactaata ttgaaaaaag
     2281 caaacaaata aaacctaagc aatataatca aactaaaaat caccaacaac aaaagaaaaa
     2341 ctaagtaatt caagctgcag ccatgccaat aaaattggtg aaattattcg ttagtaatga
     2401 actcatgaat gcttgtagcc atgaaagttc aataattgaa taatttatgg ggggaaatta
     2461 tgaagattga agactatcgt ttactaataa cattagacga aacgaaaacg ttacgtaaag
     2521 cggctgaaat tttatatata tctcaacctg ctgttacaca aagactaaaa gctattgaaa
     2581 atgcttttgg agtagatatt tttatcagaa caaaaaaaca attgattaca acaactgaag
     2641 gaacaatgat tattgagcat gcccgtgaca tgttgaaaag agagcgatta ttttttgaca
     2701 aaatgcaggc acatattggt gaagtgaatg gaacaatatc aatcgggtgt tcttctttga
     2761 ttggacaaac cttacttcct gaagttttga gcctatataa tgcccaattt cctaatgttg
     2821 aaatacaagt gcaagttggt tcaactgaac aaattaaagc aaatcataga gattatcatg
     2881 ttatgataac tcgtggaaat aaggtaatga atttagctaa cacacattta tttaatgatg
     2941 atcattattt tatttttcca aaaaatagac gagatgatgt tacaaagtta ccatttatag
     3001 agtttcaagc tgatccgatt tatataaatc aaataaaaca atggtataac gataatttag
     3061 aacaagatta ccatgcaact attacagtgg atcaagtagc aacttgcaaa gaaatgttga
     3121 ttagtggtgt aggtgttaca attttgccgg aaattatgat gaaaaatatc agcaaagaac
     3181 aatttgagtt tgaaaaagta gaaattgata atgaaccgct gattcgttcg acatttatga
     3241 gttatgatcc gagcatgttg caattgccac aagttgattc ttttgtaaat ctcatggcga
     3301 gctttgttga acaaccaaag gcgtagtttt agactaattt aaggtttgta tttaatttta
     3361 aactattcgg ttaaattgaa cgtagttgtt tgctaacgca ccaacagcaa aagagcccct
     3421 aattaataaa ttaaaagggg ctctaagaag cggggttatg gatatgtttg cagcgttatt
     3481 acaaataaag aattataaac tctttgttgc taatatgttt ctactaggta tgggtattgc
     3541 ggttacggtc ccatatcttg ttctttttgc aactaaagat ttaggtatga caacaaatca
     3601 gtatggatta cttctagcat ctgcagcgat tagccagttt acagtaaatt caattattgc
     3661 tagattttcg gatacgcatc actttaatag aaaaattatt attattctcg cattattaat
     3721 gggtgcgctt ggtttttcaa tatacttttt tgtagataca atctggttat tcatattact
     3781 atatgcgatt ttccaaggat tatttgcacc agcaatgccc caactttacg catctgctag
     3841 agaatctatc aatgtttcaa gctctaaaga tagagctcaa tttgccaaca cagtattacg
     3901 ttcaatgttc tcattgggct ttttatttgg tccatttatt ggtgcccaat taatcggttt
     3961 aaaaggctat gctggattgt ttggtggaac aataagtatc attttattta ctttagtact
     4021 tcaagtgttt ttctataagg atttaaacat taaacaccct attagtacgc aacaacatgt
     4081 tgaaaaaatt gctcctaata tgtttaaaga caaaacgctt ttattaccat ttattgcatt
     4141 tattttatta cacattggac aatggatgta tacgatgaat atgcctttat ttgttactga
     4201 ttatttaaaa gaaaatgagc aacatgtcgg ttatttagct agtttatgtg ctggtttaga
     4261 agtgccattt atgatcattc ttggcgtttt atcatctaga ttacatactc gaacattgtt
     4321 gatttatgga gcgatttttg gtggtttatt ctacttcagc attggggtat ttaaaaactt
     4381 ctatatgatg ttagcaggac aggtattttt agctattttc ttagcggttc ttttaggaat
     4441 tggtattagt tatttccaag atatcttacc agattttcca ggatacgcct caacactatt
     4501 ttctaatgca atggttattg gacagttagg cggtaaccta ttaggtggtg ctatgagtca
     4561 ctgggtaggt ttggaaaatg tattttttgt atcagcagca tcaatcatgt taggtatgat
     4621 acttatattc tttactaaaa atcaaaaaat tacaaaagag gatatgatat caacatgaca
     4681 attattttat ggctacttat catcgctgcc ttcatgttag catttgttgg gttgattaag
     4741 ccgattattc cttctgtttt agtattatgg gttggctttc taatctatca atttggcttt
     4801 cataatcagc atttatcatg ggtgttttat gtatctatgg cattgctaac aatactaatt
     4861 ttatgtgcgg actttttagc taataaatat tttgtgaatc gcttcggtgg ttctaagttt
     4921 ggagagtatg cagctttaat tggtgtggtt attggatgtt ttgttttacc gccatttgga
     4981 attattatta taccttttat tttggtattc atagttgaat taatacaagg ctattcattt
     5041 gaaagagcag ttaaagtaag tataggttca atcgtagcat ttttaacaag tagtatagct
     5101 caagcaatca ttatgtttat aatgattgta tggttcttta tagatgcttt attgattaat
     5161 taataaaaag cttattgcaa aatatgtttt tcggtaactg taatttagtg attttatcat
     5221 taacagtacc aaattcgcat atgatgtaat aagctttttg tttataaaaa tgtatgagaa
     5281 tgttttttcg aaatatttct ttcaatgcgt aatccaatag gcataactat aaatgaaaat
     5341 ataataaata cccaattgct tacctgaata tatggtccaa aggcaagtaa tgattgtgga
     5401 ataaagaata catagaaaaa tcctacaatt agtagaatga ttgctaaata ggtgaatatc
     5461 catatccaat ttgaattgtt aaaacactgc agttgtattg ttttaaaact aaaccatata
     5521 aataaaaaga caataaagaa tccaacgact actgaaaacg ggaatgaaac aaaatataaa
     5581 ttacttccat ttttttccat gaaaaatcct aaaaatcctt tgagaaaact aacaatccca
     5641 attaatagaa cgatgtgttg atagatatat ttaaaaatat ttttaaatgt ttcattaggc
     5701 atcgctttta gttcttttat tgcatgtgct tttgggtcgt gattgaaaaa atctaaggct
     5761 aataaaccat gttgttctgc gcttaataat tgtttgagta tacggttaat aattaactct
     5821 gtatcatgag ggttgacgcg aaagtcagag cgcatataag tcatataatt ctcgaagatt
     5881 tctctatcag tattgcttaa tcttaatgat ttaacattat tttcttttgt taattgcgca
     5941 gtacttttca ttgttactta agcgctcctt taaaaatgtt taattcctaa ttaaaatgga
     6001 aatgatttta tagtattaat aaagtcaatc atatcatatt aaacgcataa atataacgat
     6061 taatattgga gaggaaaatg aggacactta ataaagatga acataattat atcaagcaaa
     6121 tagctaatat acacgagaca ttattgtcgc aagcagaatc caactataaa tgtactaaac
     6181 tgagtattgc tcttaggtac gagatgatat gttcaagatt agaacataca aatgataaaa
     6241 tttatatata tgaaaatgaa ggtcaattaa tagcgtttat ttggggacat tttagtaatg
     6301 aaaaaagtat ggttaacatt gaactgctat atgttgaacc acaatttcgc aaactgggaa
     6361 tagctacgca actgaagatt gcgcttgaaa aatgggcaaa aactatgaat gcaaagcgaa
     6421 taagcagtac aattcataaa aataatttgc caatgatatc tttgaataaa gatttaggtt
     6481 atcaagtgag tcatgtgaaa atgtataaag atattgatta gaattaggat tatgttgcta
     6541 attcatgtta aaattaaaaa agatttaatg acgttaagga gttttatatg aagaaattaa
     6601 tcatcagtat tatggcggtc atgctatttt taacaggttg tggtaaaagt caagagaaag
     6661 ccactctgga aaaggatatc gataatttac aaaaagaaaa taaagaatta aaagacaaaa
     6721 aagaaaagct tcaacaagaa aaagaaaaat tagcagataa gcaaaaagac cttgaaaaag
     6781 aagtgaaaga tttaaaacct tcaaaagaag ataacaagga tgataaaaaa gacgaagaca
     6841 aaaataaaga caaagataaa gataaagagg catcacaaga taagcaatca aaagatcaaa
     6901 ctaagtcatc ggataaagat aatcacaaaa agcctacatc aacagataaa gatcaaaaag
     6961 ctaatgacaa acaccaatca taatcgtatt gcttacttgt tatagatgaa aggtacagcg
     7021 ttttaaacct tattttaagg gtatgtatta attaaaatgt ggtcatgatt gaaacagaac
     7081 gtaaaaatag acaacgtaat taataaagga gagaaacggc atgcatgaac aagattttag
     7141 aattttagag ggtcaagata ttactttgcc agaattaggt agagaattag aaaatattac
     7201 aggacatacg attgctgatt ctactggcga aattaagcgt gtaattgcac atttaccaaa
     7261 ctttgagtcc gatacagata cttttgttgc tacatatcgt ttaaaccatc aacaagattt
     7321 tatagatgca acttttactg cgctgaaatc agatagagca cgtttaaaag aagtgccagt
     7381 tcatgttgaa cttataagtt atatttctaa atcaaaataa actgctatct aaaacgcaaa
     7441 gttgatcaaa atatcgattt tgtgtttttt attgagaaat tatataggag tgtcaatcga
     7501 tgatttattg tgaaacagag cgtttaatat taagagactg gcatgaagat gatctgttac
     7561 cttttcaaaa aatgaatgcg aattatgacg tacgtaaata ttttccaagt ttattgagtt
     7621 atcgtcgttc agaattagat atgagaacta tggatgcggt tattaaagat tatggcattg
     7681 gattatttgc tgtagaagat aaagagtccc atcaatggat aggctttata ggtttgaatt
     7741 atattccaga aacaagcgat tatccattta aagaattacc gctttatgaa ataggttggc
     7801 gcttgttgcc tgaattttgg ggaaaaggat tagcaactga aggcgcaaag gcaacgttga
     7861 agttagcaga agaacatcaa atatacgatg tctatagttt tacagcagaa gcaaataaag
     7921 cctcacaacg tgtaatggaa aaaattggca tgacagtgta tgatcatttc gaattaccca
     7981 atctaagtaa gtatcattta ttaaaaaggc aagtgcgcta ttacattaat cttcgaaagt
     8041 gaaaaattta tacataatcg taacaaacac ccctaacatt gtttagctga tgataacaat
     8101 attaggggtg tttgtttatt ttttaacctt agaatgatta atcgtatgaa cgagtaccca
     8161 gaggtttgaa atttaatatt gattctatta atgattcctt agtgtcgcat aacggtgcaa
     8221 gagcacgata cttaggatca ataaaacctt cctcaatcat atggtcaatc attgtttgta
     8281 gtggattgaa aaagccatta atattataaa tggcaatagg cttttcatgg atacctattt
     8341 gagcccaact atacatttcg aaaaattctt ctagtgaacc tgcgccacca ggagccatga
     8401 caaatgcatc tgcaagttct gccattttat ttttacgttc atgcatagaa tcaactaaaa
     8461 ttaattcagt taaacgttgg cttgtgattt catgttcatc taacatttta ggcatgacgc
     8521 caatagcttt gccgccatga tttaatacac catcttgaat ggcacccata atgccaattg
     8581 accctgcacc aaatactaat tcataacctt gttcagcaaa atatttacct aaatcgtatg
     8641 ctttttgtac atatgaaggg tcatgacctt tgcttgcacc acaataaact gcgattcgtt
     8701 tcatgttaat ccagctcctt aattcgatga atgactttta atagtgattg ttcaaacact
     8761 ttttgatctt gctttgtaaa aggtggggga cctttgtggc gaccaccttg ttttctaatt
     8821 tgtgcattta tatatcgttt atctaatagt tgttgaatat ttttggaatt gtatatcttc
     8881 ccattatgat gcatgacaat taagactttg tcgactaata aacttgcgag tccataatct
     8941 tgagtgacta cgatatcatc cttcgttgat aattgaacaa ttttgtaatc aactgcatct
     9001 ggtccatcat caacatataa tgttgataca tgtggaggat ataattggtt cgaaaaatgg
     9061 ctgaagctcc gaataattgt cacaaaaatg cctgtctcag ttgttaaatc tataatagaa
     9121 tcaacaacag gacaagcatc tccatcaata ataatatgtg tcacaattat gcctctgtat
     9181 tgttttcttt attttgttga gaggcgcttt tggcaacata atctttatat tttttaaatg
     9241 acttgatgcg tgctttatca gcttcttgtt ggcgtttttg ttcttctttg tgtcgttttt
     9301 caatattttt ttgtaacttt ttattcattt tagcgatttc tttgcgattt ttttcagcta
     9361 gtttatcgcc ttttttctca gttttctcat ctaatttatt aggtgttaag cctgcttttt
     9421 cttcgtattt ttgtgatttt ttcatatctt taatacgttg tatttcattc ttttcgcggg
     9481 ctttttgctc ttctttatga cgcttttcga tatttttttg aagtatttta ttcattttat
     9541 cagcgtcttt acgattttgt ttagctaatt tttcgccttt tttctcaata taggcaggat
     9601 catgttctct agcaaacttt ttaagttcac gtttattttc aaaatcttgt tttttatcgc
     9661 cgacatattc tttaacatca ctcgctgtgt tactgattgc tgcagatgtt tttgaagcaa
     9721 ctttacttgt agcatctgta actttttgta cgtccggatg ttgtttgata cgtttacgtt
     9781 caacaattaa cggtaccaat acaattggta atacattaat cataaatttg atgacttttt
     9841 tcttatccat agatcttgcc tccataatta ctttattaat tttacatacc ctatgataca
     9901 tcaatataaa cgatgatagt agtgaatcac tattaagtat ttcagatgtt ttttaaaaga
     9961 agacaataaa aactgccaat caagtggttc cttaattgac agtctatatt ttaaaaggaa
    10021 attaaatacc tttaccaatg ccaaatccga agtaaagtat agcaataaag attactaata
    10081 caattctgta aatggcaaat ggaattagtt tgattttgtt aattagatgc aagaatgttt
    10141 tgattgcaat tagtccaaca gtaaatgcag ctaaaaagcc taaaatataa aaaggtatat
    10201 cagcaatctg aatatcttga taatgtttta ataaagataa accactagct gctaacataa
    10261 ttggaacagc cataataaat gtgaagtccg atgctgcttt atgatttaat ttcattaata
    10321 ccccagttga aattgttgag cctgaacggc tgaaaccagg ccacatagct actgcttgag
    10381 agataccaat tacaaacgct tggaaataat tgatttgatc cactgtttgt gggtttttaa
    10441 ctttaactga atacttatca gcaataatca tatagatagc acctacgaat aagccaatca
    10501 taacagttgg cacactaaat aaatgttctt cgatgaaatc atcaaatagt aagcctaaaa
    10561 tacctgctgg caccataccc actaatacat gtaataaatt taaacgtctt ggctttgaac
    10621 gtctttgttg atcgttttct ccttcaacat gtttgtgttt accaatatgt aaaatctcta
    10681 agaagcgttc gcggaatacc catgctgctg caaagacgga tcctaattgg atgacgattt
    10741 taaatgtaaa tgctgattga gaacctaaaa attcagatga ttttaaccac atatcatcaa
    10801 ctagaatcat atgtccagta gaggaaacag gtgcaaattc tgttaatcct tcgacgaccc
    10861 ctaagataat accttttatt aattcaatga taaacataat gtacccactt tcattactca
    10921 atttaattta tttaaatatc aaaattacca tatcatgata gcatattcat ttaaagacat
    10981 gctagttata gttataatac tagactaaag atgtatatat tcattttctt ttacatgtaa
    11041 aactacaata ttttattgag ctatttaatt tgattttaag gaaaaccttt tataataggt
    11101 ttaggtgata taattgtgaa aaaattaaca acaatactgt ttcaatataa aatttttccg
    11161 gtactcatgt tcttggtcag tactggtctc ggcatactcg ttataacgca aaatatttta
    11221 atagcagatt ttttagctaa aattataaga catcaatttc aaggtttatg gattgtatta
    11281 tttattttat taggtgtttt acttttaaga gcaactgtgc aatttctaaa tcaatggtta
    11341 ggtgatacat tagcatttaa agttaagcat atgcttagac agctggttat ttataaaaat
    11401 aatggtcatc caatcggtga acaaatgact atactcacag aaaacattga tggtttagca
    11461 cctttttata agagttattt gcctcaagtg ttcaaatcaa tgatggttcc gctcatcata
    11521 atcattgcaa tgtttttcat ccatttcaat accgcattaa ttatgttaat aactgcacca
    11581 tttattcctt tgttttatat tattttcggt ttgaaaacgc gagatgagtc aaaagatcaa
    11641 atgacttatt tgaatcaatt tagtcaacgg tttttaaata ttgctaaagg tttagtgacg
    11701 ttaaagctat ttaatcgtac agagcaaaca gagaagcata tttacgacga tagtactcag
    11761 tttagaactt taacaatgcg cattttacgc agtgcttttt tatcgggatt aatgctcgaa
    11821 tttataagta tgttaggtat tggattggtt gcattggaag caacgctaag cttagtagta
    11881 tttcataata ttgattttaa aactgcggca attgcgatta ttttagcgcc tgaattttat
    11941 aatgcaatta aggacttagg gcaagcgttc catactggaa aacaaagtga aggtgccagt
    12001 gacgttgtgt ttgagttttt agaacaaccg aactataata atgaatttct attaaagtat
    12061 gaggaaaacc aaaagccatt tattcagtta acagacatat catttcgata tgatgattct
    12121 gatagattgg tattaaatga tttaaatttg gaaatattta aaggtgatca aattgcactt
    12181 gtaggtccaa gcggggcagg taaatccact ttgacacatc ttattgcagg tgtttatcag
    12241 ccaacaatag gtactataag tacaaaccag cgtgatttaa atataggaat acttagtcaa
    12301 cagccatata ttttcagtgc ttctataaaa gagaatatta cgatgtttaa agatatagaa
    12361 aataatacta ttgaagaagt gctagacgaa gtaggtttat tagacaaagt gcaatctttc
    12421 acaaaaggca ttaacacaat aataggtgaa ggaggcgaaa tgttatctgg tggacagatg
    12481 agacgcatag aactttgccg tcttttagtt atgaagccag atctcgttat atttgatgag
    12541 cctgcaactg gtttagatat tcaaacagaa cacatgattc agaacgttct gtttcaacat
    12601 tttaaagata caacgatgat tgtcattgca catagagata atacaattcg ccatttacaa
    12661 cgacgcttgt atatagaaaa tggaagactg attgctgatg atcgcaatat ttcagtaaat
    12721 ataacagaaa atggtgatga cttatgaaaa cacgactaaa atttcaagta gataaggatt
    12781 tattgttagc tatagttgtt ggtgtttgtg gaagtttagt tgcgctcgcc atgtttttct
    12841 taagtggtta tatggtgaca caaagtgcac ttggtgcgcc actatacgct ctgatgattt
    12901 tagtcgttac agtaaaattg tttgggtttt taagagctat tactcgatac gtagagcgcc
    12961 ttatttctca taaagctaca tttacaatgc tacgtgatat tcgggtacag tttttcggta
    13021 aattagtaaa tgtcattcct aatgtttacc gtaaactgag ttctagtgat ttaatttcac
    13081 gtatgattag tcgtgttgag gcattacaaa atatatattt acgtgtttat tatccaccag
    13141 tcgttatcgg tttgacagcg ctagttacag tcatagtttt ggcgttcatt tcaatcggcc
    13201 atgcgctatt gattatggtt agcatgttgt ttactttact cattgttcct tggttaagct
    13261 caaaaaaagc acgtacttta aagaaacatg cagctaatga acaggcccga tttttaaatc
    13321 atttttatga ttataaagct ggtatggatg aactacgtcg atttaatcaa attaatcatt
    13381 atcgagataa tttgatggct aaattaaatc attttgataa attacaactt aaagagcaac
    13441 gctttttaac gatttatgat tttatattaa atattattgc tatgctttcg atttttggta
    13501 gtttagttct aggattaatt caaattaatg caggccaact aaatattatt tatatgacga
    13561 gtatagtttt aatgatctta actttatttg aacaagctgt accaatgaca aatgtcgcgt
    13621 attataaagc ggatactgac caagcattgc acgatattaa tgaagtgata tctgtacctt
    13681 ctactaatgg aaaaaaacgt cttaatgata agtatgatgc aacgaacatt tatgaagtta
    13741 aggatgctag ttttaagtat tggaatcagc aaacgtatgt gttgtcggat attaatttta
    13801 atgttaatag aggcgaaaag attgcgattg tgggtccttc aggttcagga aaaagtacat
    13861 tactacaaat tatggcaggg ttatatcaat tagatagtgg ctctgttcgt ttcgaaaata
    13921 tggatatgtt tgaaatagat gacaaagata agtttgaatc gttaaatgtc ttgctacaat
    13981 ctcaacaatt atttgatggt acaatacgtc aaaatttatt taccgatgaa aaagatgaag
    14041 cggtgcaagc aatatttaag caattagatt tagaacattt ggcactagaa cgtcaaattg
    14101 acttagatgg tcatacatta tctggcggag aaattcagcg tttagcgatt acgaggatgt
    14161 tattaaaaga tactgcatca acatggattt tagatgaacc aacaactgca ttagataaac
    14221 aaaatagttt aaaagttatg gatttaattg aagcacatgc agaaacatta attgttgcta
    14281 cacacgattt aactttattg tcacgttttg agaccatcat tgtgatgata aatggtaaaa
    14341 tagttgaaaa gggaaactat caacaattac tcgctaatca aggtgcttta tggaatatga
    14401 ttcaatataa tgcataaaaa aactgcttga taggctaaga aacctttcaa gcagtttttt
    14461 aactaacaag cttacgcgtg ctaattgttt ttctattctt aataaattct aaacattact
    14521 ttaattgtca tgacaaaagt taattatttt tcctttgttt catcaaatgc atgaatgact
    14581 ttacctaata agcgattaag ttctttaact tcatcttgag ataaagaaga agctgaagcg
    14641 actttgtcag atgcattact taattctggt ctaatagttt cacttttgtc agtcaagtga
    14701 ataaatactt cacgttgatc gacttcggaa cgttcacgct taattaagtc tacttgttcc
    14761 attcgtttta ataatggtga tactgtacca gtatcgagtg ctaattcagt tacgactttc
    14821 ttgacgttta caggagattc atcccataaa attgttaaga caagaaattg tgggtatgtt
    14881 agattgtact tcttaaaaac tttgttagag tagtagcgat taacttgtct ttgagcattg
    14941 tacaaactaa agcatagctg ttcttttaaa ttatgttgat cagacattaa agttctcctc
    15001 cagacatact atccgttttt ttctcttttc ggattggtaa tcattaaaaa gttgattgtt
    15061 tattaattca caactttctt tgattcaatg ccatgctaaa attaaagtat gtttaaagtt
    15121 tagaagatat tt
//
LOCUS       DANBWH010000047        15102 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.049, whole genome shotgun sequence.
ACCESSION   DANBWH010000047 DANBWH010000000
VERSION     DANBWH010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15102
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.049"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..1540
                     /gene="miaB"
                     /locus_tag="PPY92_001006"
     CDS             <1..1540
                     /gene="miaB"
                     /locus_tag="PPY92_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDJ2625024.1"
                     /translation="EEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKRGK
                     QEVRYNRDFQIDEKYRGMGNERTFLIKTYGCQMNAHDTEVIAGILEALGYQATTDINT
                     ADVILINTCAIRENAENKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILKSY
                     QNVDMIFGTHNIHHLPEILEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVNIM
                     YGCDKFCTYCIVPFTRGKERSRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDLQD
                     IEYDLGDLLQAISKIAIPRVRFTTSHPWDFTDHMIDVISEGGNIVPHIHLPVQSGNNA
                     VLKIMGRKYTRESYLDLVKRIKDRIPNVALTTDIIVGYPNESEEQFEETLTLYDEVGF
                     EHAYTYLYSQRDGTPAAKMKDNVPLNVKKERLQRLNKKVGHYSQIAMSKYEGQTVTVL
                     CEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEVRIDEAKQYSLNGSFVKEVEPE
                     MVIQ"
     gene            1541..1906
                     /locus_tag="PPY92_001007"
     CDS             1541..1906
                     /locus_tag="PPY92_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RicAFT regulatory complex protein RicA family
                     protein"
                     /protein_id="HDJ2625025.1"
                     /translation="MYNKDDVLKQADNIANKIKNLDTIKTYQQIEAQIHQNQTIKTKM
                     DMLKKHQKQAVNFQNYGKQNALEQSEHTIQSIEAEINTLPIVEQFQTSQYEANQLLKM
                     FVSTMETRLNDHNKAKHSD"
     gene            1933..2424
                     /gene="thiW"
                     /locus_tag="PPY92_001008"
     CDS             1933..2424
                     /gene="thiW"
                     /locus_tag="PPY92_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HDJ2625026.1"
                     /translation="MKSRKLAITALLIAINVVLSSIIIIPLGPVKAAPVQHFVNVLSA
                     VIVGPWYGLAQALISSILRVLFGTGTAFAFPGSMIGVLLASMFYIYRKHIFMAAVGEV
                     LGTGVIGSLICIPLAYFLGLQDFFIKPLMITFIVSSAIGSIISYFLLITLKKRGILQR
                     FIK"
     gene            2727..5345
                     /gene="mutS"
                     /locus_tag="PPY92_001009"
     CDS             2727..5345
                     /gene="mutS"
                     /locus_tag="PPY92_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDJ2625027.1"
                     /translation="MSNVTPMMQQYLKIKSEYQDCLLFFRLGDFYEMFYEDAKEASRV
                     LEITLTKRDAKKENPIPMCGVPYHSADSYIDTLVNNGYKVAICEQMEDPKQTKGMVRR
                     EVVRIVTPGTVMEQGGVDDKQNNYILSFVMNQPEIALSYCDVSTGELKVTHFNDEATL
                     LNEITTINPNEVVINDNISDNLKRQINMVTETITVRETLSSEIYSVNQTEHKLMYQAT
                     QLLLDYIHHTQKRDLSHIEDVVQYAAIDYMKMDFYAKRNLELTESIRLKSKKGTLLWL
                     MDETKTPMGARRLKQWIDRPLISKEQIEARLDIVDEFSAHFIERDTLRTYLNQVYDIE
                     RLVGRVSYGNVNARDLIQLKHSISEIPNIKALLNSMNQNTLVQVNQLEPLDDLLDILE
                     QSLVEEPPISVKDGGLFKVGFNTQLDEYLEASKNGKTWLAELQAKERQRTGIKSLKIS
                     FNKVFGYFIEITRANLQNFEPSEFGYMRKQTLSNAERFITDELKEKEDIILGAEDKAI
                     ELEYQLFVQLREEVKKYTERLQQQAKIISELDCLQSFAEIAQKYNYTRPSFSENKTLE
                     LVESRHPVVERVMDYNDYVPNNCRLDNETFIYLITGPNMSGKSTYMRQVAIISIMAQM
                     GAYVPCKEAVLPIFDQIFTRIGAADDLVSGKSTFMVEMLEAQKALTYATEDSLIIFDE
                     IGRGTSTYDGLALAQAMIEYVAETSHAKTLFSTHYHELTTLDQALPSLKNVHVAANEY
                     KGELIFLHKVKDGAVDDSYGIQVAKLADLPEKVISRAQVILSEFEASAGKKSSISNLK
                     MVENEPEINQENLNLSVEETTDTLSQKDFEQASFDLFENDQESEIELQIKNLNLSNMT
                     PIEALVKLSELQNQLK"
     gene            5358..7367
                     /gene="mutL"
                     /locus_tag="PPY92_001010"
     CDS             5358..7367
                     /gene="mutL"
                     /locus_tag="PPY92_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDJ2625028.1"
                     /translation="MGKIKELQTSLANKIAAGEVVERPSSVVKELLENAIDAGATEIS
                     IEVEESGVQSIRVVDNGSGIEAEDLGLVFHRHATSKLDQDEDLFHIRTLGFRGEALAS
                     ISSVAKVTLKTCTDNANGNEIYVENGEILNHKPAKAKKGTDILVESLFYNTPARLKYI
                     KSLYTELGKITDIVNRMAMSHPDIRIALISDGKTMLSTNGSGRTNEVMAEIYGMKVAR
                     DLVHISGDTSDYHIEGFVAKPEHSRSNKHYISIFINGRYIKNFMLNKAILEGYHTLLT
                     IGRFPICYINIEMDPILVDVNVHPTKLEVRLSKEEQLYQLIVSKIQEAFKDRILIPKN
                     NLDYVPKKNKVLYSFEQQKIEFEQRQNTENNQEKTFSSEESNSKSFMAENQNDEIVIK
                     EDSYNPFVTKTSESLITDDESSGYNNTREKDEDYFKKQQEILQEMDQTFDSNEDASVQ
                     NYENKASDDYYDVNDIKGTKSKDPKRRIPYMEIVGQVHGTYIIAQNEFGMYMIDQHAA
                     QERIKYEYFRDKIGEVTNEVQDLLIPLTFHFSKDEQLVIDQYKNELQQVGIMLEHFGG
                     HDYIVSSYPVWFPKDEVEEIIKDMIELILEEKKVDIKKLREDVAIMMSCKKSIKANHY
                     LQKHEMSDLIDQLREAEDPFTCPHGRPIIINFSKYELEKLFKRVM"
     gene            7373..7915
                     /locus_tag="PPY92_001011"
     CDS             7373..7915
                     /locus_tag="PPY92_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HDJ2625029.1"
                     /translation="MNQVNNNILPAIRNIKDLEKLIKTDYKMCVLLDMHIGHIKSIME
                     LLKQNHIECFIHIDLIKGLSHDEFASEFIIQQYKPKGIVSTKSKVIKKAKSLNTLTIF
                     RVFIIDSQALKRSIDLIKKVEPDFVEVLPGVASKAIHHIQKETNTQVIAGGLINTIDE
                     VNEAVKNGAKYVTTSYDKLW"
     gene            8390..9208
                     /locus_tag="PPY92_001012"
     CDS             8390..9208
                     /locus_tag="PPY92_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDJ2625030.1"
                     /translation="MNVYLAEFLGTAILILFGGGVCANVNLKRSAANGADWIVITAGW
                     GLAVTMGVFAVGQFSGAHLNPAVSLALALDGSFDWSLVPGYIVAQMLGAIVGATIVWL
                     MYLPHWKATEEAGAKLGVFSTAPAIKNYFANFLSEIIGTMALTLGILFIGVNKIADGL
                     NPLIVGALIVAIGLSLGGATGYAINPARDLGPRIAHAILPIAGKGGSNWSYAIVPILG
                     PIAGGLLGAVVYAVFYKHTFNIGCAIAIVVVIITLILGYILNKSSKKGDIESIY"
     gene            9337..10833
                     /gene="glpK"
                     /locus_tag="PPY92_001013"
     CDS             9337..10833
                     /gene="glpK"
                     /locus_tag="PPY92_001013"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDJ2625031.1"
                     /translation="MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWV
                     EHDANEIWTSVLAVMTEVINENDVRADQIAGIGITNQRETTVVWDKHTGRPIYHAIVW
                     QSRQTQSICSELKQQGYEQTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGDLL
                     FGTIDTWLVWKLSGKAAHITDYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKP
                     SSEIYGKTIDYHFYGQEVPIAGVAGDQQAALFGQACFECGDVKNTYGTGGFMLMNTGD
                     KAVKSESGLLTTIAYGIDGKVNYALEGSIFVSGSAIQWLRDGLRMINSAPQSESYATR
                     VDSTEGVYVVPAFVGLGTPYWDSEARGAIFGLTRGTEKEHFIRATLESLCYQTRDVME
                     AMSKDSGIDVQSLRVDGGAVKNNFIMQFQADIVNTSVERPEIQETTALGAAFLAGLAV
                     GFWESKDDIAKNWKLEEKFDPKMDEGEREKLYRGWKKAVEATQVFKTE"
     gene            10991..12664
                     /locus_tag="PPY92_001014"
     CDS             10991..12664
                     /locus_tag="PPY92_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HDJ2625032.1"
                     /translation="MALSTFKREHIKKNLRNDEYDLVIIGGGITGAGIALDASERGMK
                     VALVEMQDFAQGTSSRSTKLVHGGLRYLKQFQIGVVAETGKERAIVYENGPHVTTPEW
                     MLLPMHKGGTFGKFSTSIGLGMYDRLAGVKKSERKKMLSKKETLAKEPLVKKEGLKGG
                     GYYVEYRTDDARLTIEVMKRAAEKGAEIINYTKSEHFTYDKNQQVNGVKVIDKLTNEN
                     YTIKAKKVVNAAGPWVDDVRSGDYARNNKKLRLTKGVHVVIDQSKFPLGQAVYFDTEK
                     DGRMIFAIPREGKAYVGTTDTFYDNIKSSPLTTQEDRDYLIDAINYMFPSVNVTDEDI
                     ESTWAGIRPLIYEEGKDPSEISRKDEIWEGKSGLLTIAGGKLTGYRHMAQDIVDLVSK
                     RLKKDYGLTFSPCNTKGLAISGGDVGGSKNFDAFVEQKVDVAKGFGIDEDVARRLASK
                     YGSNVDELFNIAQTSQYHDSKLPLEIYVELVYSIQQEMVYKPNDFLVRRSGKMYFNIK
                     DVLDYKDAVIDIMADMLDYSPAQIEAYTEEVEQAIKEAQHGNNQPAVKE"
     gene            12814..13728
                     /locus_tag="PPY92_001015"
     CDS             12814..13728
                     /locus_tag="PPY92_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDJ2625033.1"
                     /translation="MTEKQFKLTVQDNTNIEVKVNFTDVDSKGIIHIFHGMAEHMERY
                     DKLAHALSKHGFDVIRHNHRGHGINIDESTRGHYDDMKRVIGDAFEVAQTVRGNVDKP
                     YIIIGHSMGSVIARLFVETYPQYVDGLILSGTGMYSLWKGLPTVKVLQLITKIYGAEK
                     RVEWVNQLVSNSFNKKIRPLRTQSDWISSNPIEVDNFIKDPYSGFNVSNQLLYQTAYY
                     MLHTSQLKNMKMLNHAMPILLVSGYDDSLGDYGKGILKLANIYRKAGIKNVKVNLYHH
                     KRHEVLFEKDHDKIWEDLFKWLNQFYKK"
     gene            13746..14681
                     /gene="miaA"
                     /locus_tag="PPY92_001016"
     CDS             13746..14681
                     /gene="miaA"
                     /locus_tag="PPY92_001016"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDJ2625034.1"
                     /translation="MNKNKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYK
                     HMNIGTAKVTPEEMDGIPHHLIDILNPDDTFSAYEFKRLAEDLITDITNRGKVPIIAG
                     GTGLYIQSLIYNYELEDETVTPAQLSIVKQKLSALEHLDNQQLHDYLAQFDAVSAENI
                     HPNNRQRVLRAIEYYLKTKKLLSNRKKVQQFTENYDTLLLGIEMSRKTLYSRINKRVD
                     IMLDHGLFREVQQLVEQGYESCQSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYA
                     KRQMTWFKNKMSVHWLDKENMSLQMMLDEITTQIK"
     gene            14696..14929
                     /gene="hfq"
                     /locus_tag="PPY92_001017"
     CDS             14696..14929
                     /gene="hfq"
                     /locus_tag="PPY92_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDJ2625035.1"
                     /translation="MIANENIQDKALENFKANQTEVTVFFLNGFQMKGVIEEYDKYVV
                     SLNSQGKQHLIYKHAISTYTVETEGQASTESEE"
BASE COUNT         5680 a         2001 c         2886 g         4535 t
ORIGIN      
        1 cgaagaacaa agaaaagcaa gttctgtaga tgttttagct gagagagata agaaagcaga
       61 aaaagattat agtaaatatt ttgaacatgt ttatcagccg cctaatttaa aagaagcgaa
      121 aaaaagaggt aaacaagaag ttcgttataa tagagatttc caaattgatg aaaaatatcg
      181 cggtatgggg aacgagcgta catttttaat taaaacatat ggatgtcaaa tgaatgcaca
      241 tgacactgag gtcattgctg gtatacttga agcattaggc tatcaagcaa cgactgatat
      301 taacactgca gatgttattt taattaatac atgtgcgatt agagaaaatg ccgagaacaa
      361 agtgtttagt gaaataggta atttgaagca tttgaaaaaa gaacgacctg atattttaat
      421 cggtgtttgt ggttgtatgt cacaagaaga gtcagtagtg aataaaattt taaaatcgta
      481 tcaaaatgta gatatgatat ttggtacaca taatattcat catttaccag aaattttaga
      541 agaagcatac ttatctaaag caatggttgt tgaagtatgg tctaaagaag gagacgttat
      601 tgaaaatctt ccaaaagtcc gtgaaggcaa cattaaagca tgggtcaata ttatgtatgg
      661 ttgtgataag ttttgtacat attgtattgt tccatttaca agaggtaaag aacgaagccg
      721 tagacctgaa gacattatag atgaagtacg tgaacttgct cgtgaaggtt acaaagaaat
      781 aacgctttta ggtcaaaatg taaattctta tggtaaagat ttacaggata tagaatatga
      841 cttaggagat cttttacaag caatttctaa aatagcgatt ccaagagttc gtttcacaac
      901 aagtcatcct tgggacttta cagatcacat gattgatgtt atttcagagg gtggtaatat
      961 cgttcctcat atccacttgc cagttcaatc tggaaataat gcagtattaa aaataatggg
     1021 tagaaaatat acacgagaaa gttatttgga tttagtaaaa cgaatcaaag atagaattcc
     1081 taatgtagca ttaactacag atattattgt agggtatcca aatgaatcag aggaacaatt
     1141 tgaagaaact ttaactctgt atgatgaagt tggttttgaa catgcatata cgtacttgta
     1201 ttcacaacgt gatggtacgc ctgctgctaa aatgaaagat aatgtacctt taaatgtcaa
     1261 aaaggaacga ttgcaacgtt tgaataaaaa agttggtcat tattcacaaa tagctatgag
     1321 taagtacgaa ggacaaactg taacagtact ttgtgaaggt agtagtaaaa aagatgatca
     1381 ggttcttgct ggctacactg ataaaaataa gctagttaat ttcaaagcac ctaaagaaat
     1441 gattggtaaa ctagtggaag tacgaataga tgaagctaaa cagtattcat taaatggcag
     1501 ttttgtaaag gaagtagagc cggaaatggt gattcaataa atgtataata aagatgacgt
     1561 gttgaaacaa gcggataata ttgcaaataa aattaaaaat ttggatacta tcaaaacata
     1621 tcaacaaatt gaagcacaga ttcatcagaa ccaaacgata aagactaaaa tggatatgtt
     1681 aaaaaagcat caaaaacaag cagtaaactt tcaaaattac gggaaacaaa atgcgctaga
     1741 acagtcggaa cataccattc aaagtataga agcagaaata aatacattgc ccatagttga
     1801 acagtttcaa acttcacaat atgaagcgaa tcaattattg aaaatgtttg tatcaacaat
     1861 ggaaacacgt ttaaatgacc ataataaagc caagcatagt gattaattac aaacaaaaag
     1921 gagaacaatc atatgaaatc aagaaaactg gctataactg cacttttaat tgcaataaat
     1981 gttgtattaa gcagtattat catcattcct ctaggaccag ttaaggcagc accagtacag
     2041 cattttgtaa atgtattaag tgcggtcata gtaggtcctt ggtatggatt agctcaagcg
     2101 cttatatcat caattttaag agttcttttt ggtactggta cagcttttgc atttccaggt
     2161 agtatgattg gagttttatt ggctagtatg ttttacatat atcgtaaaca tatattcatg
     2221 gccgcggtcg gtgaagtact tggaactggt gtcatcggaa gtttaatttg tataccatta
     2281 gcatatttcc ttgggcttca agacttcttc attaaaccgc taatgattac gttcatagtc
     2341 tcaagtgcta tcggatctat tataagttat ttcttattaa ttactctaaa aaaacgtggt
     2401 attcttcaaa ggtttataaa ataattttat gatatatgat ttcaaacaga gtcattgttc
     2461 attttaatat tgaacgccct tacttaatta aatgtcttga atgttagaat tttaatttaa
     2521 ttctaaacat gctattgaca cattttatta ggtttgaggc gttttcttat acatttaaga
     2581 tgcttcgtga ttatatggta tgaataggtt tgtaaaagta agtatgttaa aatgtataag
     2641 ttaaataatt aataattaga aagcttacaa acgaatatat tgtaaagaca aattttaaaa
     2701 agagacaagg gttgtgaaga aataatatgt ctaatgttac accaatgatg cagcaatatt
     2761 taaaaataaa atcagaatac caagattgct tattattttt tagactaggt gatttctatg
     2821 aaatgtttta tgaagatgcc aaggaggcat cacgtgtact tgaaattact ttaactaaaa
     2881 gagatgctaa aaaagaaaat ccaattccga tgtgtggtgt tccgtatcat tctgcagata
     2941 gttatataga tacacttgtt aataatggat ataaagtagc tatttgtgaa cagatggaag
     3001 atccgaaaca aacgaaaggt atggttagac gtgaggtagt aagaattgtg actccaggaa
     3061 ctgtgatgga gcaaggtggt gtagatgata aacaaaataa ctatatttta agttttgtta
     3121 tgaatcaacc tgaaattgcg cttagttact gtgatgtttc tactggcgaa ttaaaggtta
     3181 cacattttaa tgatgaagcg actttattaa atgaaattac gacgataaac cctaacgaag
     3241 ttgttatcaa tgacaatatt tccgataatt taaaaagaca aattaatatg gtgacagaaa
     3301 caataacagt cagggaaacg ttatcatcag aaatctatag tgtgaatcaa actgaacata
     3361 aattaatgta tcaagcgaca caattattgc tagattatat tcatcataca caaaaacgtg
     3421 atttatcgca tatcgaggat gttgttcaat atgcagctat agattatatg aaaatggatt
     3481 tttatgctaa gagaaacctt gagttaacgg aaagcattcg attaaaatca aaaaaaggaa
     3541 cgctactttg gctaatggac gaaacgaaaa caccaatggg agcacgccgc ttaaaacaat
     3601 ggatagatag accactaata agtaaagaac aaattgaagc acgattagat atcgttgatg
     3661 aatttagtgc tcatttcata gaaagagaca ccttaagaac atatcttaat caagtgtatg
     3721 atattgaacg tcttgttggg cgtgttagtt acggaaatgt taatgcgaga gatttaattc
     3781 aacttaaaca ttccatttct gaaataccga atattaaagc attactaaat tctatgaatc
     3841 agaatacgct tgtacaagtt aatcaactag aaccccttga tgatttactt gatatattag
     3901 aacagagttt agtagaagaa ccaccaattt cagttaaaga tggcggacta ttcaaagttg
     3961 gttttaatac gcaattagat gaatatcttg aagcttcaaa aaacggaaaa acatggttag
     4021 cagaattaca agccaaagaa agacaacgta caggaataaa atcattgaaa ataagcttta
     4081 ataaagtgtt tggttatttt atagaaataa cacgtgccaa cttgcaaaat tttgaaccaa
     4141 gtgaatttgg ttatatgagg aagcaaacgt tatcgaatgc tgaacgtttt ataactgatg
     4201 aacttaaaga aaaagaagat atcattttag gtgcggaaga caaagccatc gaattagaat
     4261 atcaattatt tgttcagcta cgtgaagaag ttaaaaaata tactgaacgt ttacaacaac
     4321 aagctaaaat tatttcagag ctagattgtt tacagagctt tgcagaaatt gctcaaaaat
     4381 ataattacac taggccttca tttagtgaaa ataaaacatt agaattagtg gaatctaggc
     4441 acccagtagt ggaaagagta atggattata atgactatgt gcctaataat tgtcgattag
     4501 ataatgaaac atttatatat ttaattacag gtccgaatat gtctggtaaa tcgacatata
     4561 tgagacaagt tgccataatt agtataatgg cccaaatggg agcttatgtc ccttgtaaag
     4621 aggcagtgtt acctatattt gatcaaatat tcactagaat aggtgcggca gatgatttgg
     4681 tttcaggtaa gagtacgttt atggtagaaa tgctagaagc acaaaaggca ttaacttatg
     4741 caacagagga tagtttgatt attttcgatg aaattggacg tggtacttca acgtatgacg
     4801 gtttagcttt agcgcaggca atgatagagt atgtagctga aacatcgcat gctaaaacgt
     4861 tattttcaac acattatcat gaattgacaa cattagatca agcattacca agtctaaaaa
     4921 atgttcacgt cgctgctaat gaatataaag gtgaacttat attcttgcat aaagtcaaag
     4981 atggtgcagt tgacgatagt tatggtattc aagttgcgaa attagctgat ttacctgaaa
     5041 aagttattag cagagcacaa gtgatcctaa gcgagtttga agcgtctgct ggtaaaaaat
     5101 catcgatatc aaatttaaaa atggtcgaaa atgaacctga aattaatcaa gaaaatttaa
     5161 acttaagtgt tgaagaaaca actgatactt tatctcaaaa agactttgaa caagcatcat
     5221 ttgatttgtt tgaaaatgat caagaaagcg agattgaact acaaattaaa aatttgaatt
     5281 tatctaatat gacaccaatt gaggcattgg tgaagttaag tgaattacaa aatcaattaa
     5341 aatagaggtg ttgcaaaatg gggaaaatta aagaactcca aacctcatta gcaaataaaa
     5401 tcgcagcagg tgaagtagtt gaaagaccga gttctgttgt gaaagaactg ttggaaaatg
     5461 ctatagatgc aggcgctaca gaaataagca ttgaagtaga ggaatctggc gtccaatcta
     5521 ttcgcgtagt cgataatgga agcggaattg aagcggaaga cttaggatta gtatttcata
     5581 gacatgcgac tagtaaatta gatcaagatg aagatttatt tcatattagg acattaggat
     5641 tccgtggtga agcactagcc agtatttcat cagttgctaa agtaacattg aagacttgca
     5701 cggataatgc taatggaaat gaaatatatg tagaaaatgg tgaaatatta aatcataagc
     5761 ctgcaaaagc gaaaaaagga acagatatac ttgtagaatc attattttat aatacaccag
     5821 cacgtttaaa atatattaaa agtttataca ctgaactagg taaaataaca gatattgtca
     5881 atagaatggc tatgagccat ccggacattc gaatagcact catttcagat ggcaaaacaa
     5941 tgttaagtac aaatggttca ggacgaacta atgaagtgat ggcagagatt tatgggatga
     6001 aagttgcacg agatttagta catatatctg gagatacaag tgattatcac attgaaggtt
     6061 ttgttgcaaa gcctgaacat tctagaagta ataagcacta tatttctatt tttattaatg
     6121 gacgatacat taaaaacttt atgctaaata aagcgatttt agaaggctat catacactct
     6181 taacaatagg taggttcccg atttgttata ttaatattga aatggatcca atcttagtag
     6241 acgtaaatgt tcatccaaca aaactagaag tgcgtttatc aaaagaagag caactatatc
     6301 aattgatagt gagcaaaata caagaagcat ttaaagaccg tatattaatt cctaaaaata
     6361 acttggatta tgtgccaaaa aaaaataaag tattatattc attcgaacaa caaaaaatcg
     6421 aatttgaaca aagacaaaac acagagaata accaagagaa gacgttttca tctgaagaaa
     6481 gtaacagtaa gtcatttatg gcagaaaatc aaaacgatga gatagttata aaagaagatt
     6541 catataatcc attcgtaacg aaaacatctg aaagtttaat aactgatgat gaatcttctg
     6601 gttataataa tacacgtgaa aaagatgaag actacttcaa aaaacaacaa gaaattttac
     6661 aagaaatgga tcaaacattt gattcaaatg aggatgcatc tgtgcaaaat tatgagaata
     6721 aagcgtctga tgattattat gatgtaaacg atattaaagg aacaaaaagt aaagacccta
     6781 aacgaagaat tccatatatg gaaattgttg gccaagtaca tgggacgtat attattgctc
     6841 aaaatgaatt tggcatgtac atgattgacc agcatgcagc tcaagaaaga ataaaatatg
     6901 aatattttcg agataaaata ggtgaagtta ccaatgaagt acaagattta ttaatcccgt
     6961 taacatttca tttttcaaag gatgaacaat tagttattga tcaatataaa aatgagcttc
     7021 aacaagtagg tatcatgtta gaacattttg gtggtcatga ttatattgta agtagctatc
     7081 cagtttggtt ccctaaagat gaagtagaag aaatcattaa agatatgatt gagctaattt
     7141 tggaagagaa aaaagtagat attaaaaaat tacgtgaaga tgtagcaatc atgatgtcat
     7201 gtaaaaaatc tattaaagcg aatcattatt tacaaaaaca tgaaatgtct gatttaattg
     7261 atcaattaag agaagcggaa gatccattta catgtccaca tggtcgtcca ataatcatta
     7321 atttttcaaa atacgaatta gaaaaattat ttaagcgtgt gatgtagaga ggatgaatca
     7381 agtgaataac aacatattgc ctgctataag aaacattaaa gatttagaga aactgattaa
     7441 aacagattat aaaatgtgtg tgcttctaga tatgcatata ggacatataa aaagtattat
     7501 ggaattgctg aagcaaaatc atatagagtg ttttattcat atagatttga taaaaggttt
     7561 aagccacgat gaatttgcaa gtgaatttat tattcagcaa tacaagccaa aaggtatcgt
     7621 atcgactaaa tctaaagtaa taaaaaaagc taaatcatta aatactttaa cgatttttag
     7681 agtatttatt attgatagtc aagcattgaa acgcagtata gatttgataa aaaaagtgga
     7741 acctgatttt gttgaagtac ttccaggtgt tgcgagtaaa gcgattcatc atattcagaa
     7801 agaaacaaac acacaagtca ttgcaggtgg cctaattaat acaatagatg aagtcaatga
     7861 agctgttaaa aatggagcga aatatgtaac aactagttat gataaacttt ggtaaatcat
     7921 aagtctgcaa aaatttcatt tttcacgaga tttagtcata taacataata aaaaagccgt
     7981 tttcatatgg gatatgtaat aaggtattta aggtatcata caaagaataa ttaaataaca
     8041 taattagcac ctctaaaacg attaaaatca ttaaagtttt agaggtgttt attagtaaaa
     8101 aatgcaacta gtcataaaaa atatggctga agaatgtcaa ttaaacaaaa tggcgtcata
     8161 aataaattag tgcggattga tatttctagt tttcaaatta aactactttt ataaagactt
     8221 gaaaaattaa atttgacaaa atttttacaa aaaccattga caacgctttc atatcgatag
     8281 tatcataaca aatataataa aagttaatac atagaataga gacgggagat ttctacgagc
     8341 caaactgcta gtgtaggaat ctctttgtct ttttgggagg acatttaata tgaatgtata
     8401 tttagcagaa ttcctaggaa ctgcaatctt aatccttttt ggtggtggcg tttgtgccaa
     8461 tgtcaattta aagagaagtg ctgcgaatgg tgctgattgg attgtcatca cagctggatg
     8521 gggattagcg gttacaatgg gtgtgtttgc tgtcggtcaa ttctcaggtg cacatttaaa
     8581 cccagcggtg tctttagctc ttgcattaga cggaagtttt gattggtcat tagttcctgg
     8641 ttatattgtt gctcaaatgt taggtgcaat tgtcggagca acaattgtat ggttaatgta
     8701 cttgccacat tggaaagcga cagaagaagc tggcgcgaaa ttaggtgttt tctctacagc
     8761 accggctatt aagaattact ttgccaactt tttaagtgag attatcggaa caatggcatt
     8821 aactttaggt attttattta tcggtgtaaa caaaattgcc gatggtttaa atcctttaat
     8881 tgtcggagca ttaattgttg caatcggatt aagtttaggc ggtgctactg gttatgcaat
     8941 caacccagca cgtgatttag gtccgagaat tgcacatgcg attttaccaa tagctggtaa
     9001 aggtggttca aattggtcat atgcaatcgt tcctatctta ggaccaattg ccggtggttt
     9061 attaggtgca gtggtatacg ctgtatttta taaacataca tttaatattg gttgtgcaat
     9121 tgcaattgtt gtagttatta ttactttgat tttaggttac attttaaata aatcatcaaa
     9181 aaaaggtgat atcgaatcaa tttactaaaa taaaaagaaa cgtaaatagc ataatttaac
     9241 atgtttgatt catggattat gctatttttt cgccaaaatt taacagattt tgtacaatgg
     9301 gttagcgatt attttttaat aaaggagata ctactaatgg aaaaatatat tttatctata
     9361 gaccaaggaa caacaagctc aagagcgatt ttattcaatc aaaaagggga aattgcaggg
     9421 gtagcacaac gtgagtttaa gcaatatttt ccacaatcag gttgggttga acatgatgca
     9481 aatgaaattt ggacatctgt gttagctgta atgacggaag taattaatga aaatgatgtt
     9541 agagctgatc aaattgcagg aatcggtatt acaaaccaac gtgaaacaac ggttgtttgg
     9601 gacaaacata ctggccgccc aatttatcac gcaattgttt ggcaatcacg tcaaacacaa
     9661 tcaatttgtt cagaattaaa acaacaagga tatgaacaaa catttagaga taagacagga
     9721 ttacttttag atccgtattt tgcaggtaca aaagttaaat ggattctaga caatgttgaa
     9781 ggtgcacgag aaaaagcaga aaatggcgat ctattatttg gaacgattga tacttggtta
     9841 gtatggaaat tatcaggaaa agctgcgcat attactgatt attcaaatgc gagtcgtaca
     9901 ttaatgttta atatccatga tttagaatgg gacgatgagt tattagaact acttacagta
     9961 cctaaaaata tgttgccaga agttaaacct tcaagtgaaa tatatggtaa gacaattgat
    10021 taccacttct atggtcaaga agtaccaatc gctggtgtag ctggtgatca acaagcagca
    10081 ttatttggac aagcttgctt cgaatgtggt gacgtgaaaa acacatatgg aactggtggc
    10141 ttcatgttaa tgaatacagg tgacaaagcg gttaaatctg aaagtggttt attaacaaca
    10201 attgcttatg gtattgatgg aaaagtaaat tatgcgcttg aaggttccat ctttgtttcg
    10261 ggttcagcaa tccaatggtt acgtgatgga ttaagaatga ttaattcagc accacaatca
    10321 gaaagttatg cgacacgagt tgactctact gagggtgttt atgttgttcc agcttttgta
    10381 ggtttaggaa caccatattg ggattctgaa gcacgtggtg cgattttcgg tttaacacgt
    10441 ggaactgaaa aagagcactt tatccgtgca actttagaat cattatgtta ccaaactcgt
    10501 gacgttatgg aagcaatgtc aaaagactct ggtattgatg tccaaagttt acgtgtcgat
    10561 ggtggtgcag ttaaaaataa ctttattatg cagttccaag cagacattgt taatacttct
    10621 gttgaaagac ctgaaattca agaaactaca gctttaggtg ctgcattttt agcaggttta
    10681 gcagttggat tctgggagag taaagatgat atcgctaaaa actggaaatt agaagaaaaa
    10741 ttcgatccga aaatggatga aggcgaaaga gaaaaattat atagaggttg gaaaaaagct
    10801 gttgaagcaa cacaagtttt taaaacagaa taaacttgta gattagactt ttgtataaac
    10861 attgtgatac aatcaattta agttaatatt tgaatcgaga aacgagagat ttgttcgaac
    10921 atgtacaatt gaaggattgt ttatgtggga caagtctctc gtttttacat tttttaggag
    10981 gcgttttgga atggcattgt ctacttttaa gagagaacat attaaaaaga atttaagaaa
    11041 tgatgaatat gatttagtaa ttattggtgg cggtattaca ggtgcaggta ttgcactaga
    11101 cgcgagtgaa agaggaatga aagttgcatt agttgaaatg caagactttg cacaaggaac
    11161 aagctcaaga tctacaaaat tagtccatgg tggtttacgt tacttaaaac aattccaaat
    11221 tggagtagtt gccgaaactg gtaaagaacg tgcgattgtt tatgaaaatg ggcctcatgt
    11281 tacgactcca gagtggatgc ttttaccaat gcataaaggt ggaacatttg gtaaattctc
    11341 aacatcaatt ggtttaggaa tgtatgatcg tttagcaggt gttaagaagt ctgaacgtaa
    11401 aaaaatgtta tctaaaaaag aaactttagc taaagaacca ttagttaaaa aagaaggtct
    11461 aaaaggcggc ggttactatg ttgaatatcg tactgacgat gcgcgtttaa ctattgaagt
    11521 tatgaagcgt gctgctgaaa aaggcgcaga aattatcaac tatactaaat ctgaacactt
    11581 cacttatgat aaaaatcaac aagtaaatgg tgttaaagtt atagataaat taactaatga
    11641 aaattataca attaaggcta aaaaagtggt taatgcagca ggcccatggg ttgatgatgt
    11701 tagaagtggt gattatgcac gcaataataa aaaattacgt ttaactaaag gtgtacatgt
    11761 tgttattgat caatcaaaat tcccattagg tcaagcagta tactttgata ctgaaaaaga
    11821 tggaagaatg atttttgcaa ttccacgtga aggaaaagcg tatgtaggta ctacagatac
    11881 attctatgac aatatcaaat cttcaccatt aactacacaa gaagacagag actatttaat
    11941 cgatgcgatt aattacatgt tccctagtgt taatgttaca gatgaagata ttgaatcaac
    12001 atgggcagga attagaccat taatttacga agaaggcaaa gacccttctg aaatctctcg
    12061 taaggatgaa atttgggaag gtaaatcagg tttattaact attgcaggtg gtaaattaac
    12121 aggctatcgt cacatggctc aagacattgt tgatttagta tctaaacgct tgaaaaaaga
    12181 ctacggttta acatttagtc catgtaatac aaaaggtctg gcaatttcag gtggcgatgt
    12241 aggtggtagc aagaactttg atgcgtttgt agagcaaaaa gtagatgtag ctaaaggatt
    12301 cggcattgat gaagatgttg caagacgttt agcatctaaa tatggttcaa atgttgatga
    12361 attgttcaac attgcgcaaa catctcaata ccatgatagc aagttaccat tagaaattta
    12421 tgtagaactt gtttatagta ttcaacaaga aatggtatac aaacctaacg atttcttagt
    12481 tcgtcgttct ggtaaaatgt atttcaatat taaagatgta ttagattata aagatgctgt
    12541 catcgatatt atggcagata tgcttgatta ctctccagct caaattgaag catatactga
    12601 agaagttgag caagcaatta aagaagcgca acatggaaat aatcaaccag cagttaaaga
    12661 ataattaatt tgtacaatca taaactggtg tcctgtttta agggcatcag tttttttata
    12721 cgagatacat tagtcaattg aaactatgca tcactaaatg tatgataata ataaaagcga
    12781 atataaaaac gttgttatta aaggtgtgag atgatgactg aaaaacaatt taaattaact
    12841 gtacaagata atacgaatat tgaagttaaa gtgaatttta cagatgtaga ttcaaaagga
    12901 attattcata tatttcatgg tatggctgaa catatggaac gttacgataa attagcacat
    12961 gcactttcaa agcatggctt cgatgtgata cgtcataatc atcgaggaca tggtattaat
    13021 attgatgaat caacaagagg gcattacgat gatatgaaac gagttatcgg tgatgccttt
    13081 gaagtagcgc aaacagtgag aggcaatgtt gataaaccat acattataat cggacattca
    13141 atgggatccg ttatagctag attgtttgta gaaacatatc cgcaatatgt tgatggtcta
    13201 attttaagtg gtactggtat gtattcatta tggaaaggtt taccaaccgt taaagtgtta
    13261 caactgatta caaaaattta tggtgctgag aaacgagttg aatgggttaa ccagttagta
    13321 tcaaatagtt ttaataaaaa aatacgtcca ttacgtacac aaagtgattg gatttctagt
    13381 aatccaattg aagtagataa ctttattaaa gatccatata gtggatttaa tgtgtcaaat
    13441 caattattat atcaaacagc ctattatatg ctacatacat cacaattaaa aaatatgaaa
    13501 atgttaaatc atgccatgcc tatattatta gtttcaggat atgacgattc tttaggtgat
    13561 tatggtaaag ggattttaaa attggcgaat atatatagaa aagctggcat taaaaatgtt
    13621 aaagtgaatc tttatcatca taaacgtcat gaagtgttat ttgaaaaaga tcatgacaaa
    13681 atttgggaag acttgtttaa atggttgaat caattttata aaaaataaag aaagtggaat
    13741 taaatatgaa taaaaataag ccttttattg tagtaattgt ggggccaact gcttcaggta
    13801 aaacagagct tagcatagaa ctcgcgaagc gtatcaatgg tgaaatcata agcggtgatt
    13861 ctatgcaagt ttacaaacat atgaatattg gaactgcaaa agtaacacct gaagaaatgg
    13921 atggtattcc acatcattta attgatatct tgaatcctga tgatacattt tcagcatatg
    13981 aattcaagcg attagcagaa gatttaatta ctgatataac gaatagaggt aaagttccaa
    14041 tcatagcagg tggaacaggc ttatatattc aatcattaat atataattat gaattagaag
    14101 atgaaacagt tacacctgca caattatcca tagttaaaca aaagttatct gcattagaac
    14161 atttagataa tcagcaacta cacgattatt tagctcaatt tgatgcggtt tctgcagaaa
    14221 atattcaccc taacaaccgc caaagagtgt tgcgcgctat tgaatattat ttaaaaacaa
    14281 aaaaactttt gagtaatcgc aagaaagtgc aacaatttac tgaaaattat gatacattat
    14341 tattagggat tgaaatgtcg cgtaaaacat tatattcaag aataaataaa cgtgttgata
    14401 ttatgttgga tcacggatta tttagagaag tgcaacaact tgttgaacaa ggctatgaat
    14461 cttgccaaag tatgcaagct attggatata aagaattaat acctgtgatt aacggacaaa
    14521 tgatttatga agatgctgtc aatgatttaa agcaacattc acgccaatat gcaaaacgac
    14581 aaatgacatg gttcaagaat aaaatgagtg ttcattggtt agataaagaa aatatgtcac
    14641 ttcaaatgat gttagatgag attacaaccc agattaagta aaaggagtcc gacagatgat
    14701 tgcaaacgaa aacatccaag acaaagcact agagaatttt aaagcaaacc aaactgaagt
    14761 aactgtattc tttctaaacg gtttccaaat gaaaggtgtt attgaagaat acgacaagta
    14821 tgtcgtaagc ttaaattctc aaggcaaaca acacttgatt tacaaacatg cgatcagcac
    14881 ttatacagta gaaactgaag gtcaagcatc tactgaaagt gaagaataag ttgtaatcat
    14941 atgggcacga tttaatgact cgtgtacata acgtttgaag tatgataaat agatatagaa
    15001 ataaagtcat atgtgacatc aattaaatga tgttcaaatg acaagataca atagaggaat
    15061 gtttgtaata aaaacgcttc atataaaggt cgagtcaata ta
//
LOCUS       DANBWH010000048        15061 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.050, whole genome shotgun sequence.
ACCESSION   DANBWH010000048 DANBWH010000000
VERSION     DANBWH010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15061
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.050"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(4..1104)
                     /locus_tag="PPY92_001018"
     CDS             complement(4..1104)
                     /locus_tag="PPY92_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDJ2625036.1"
                     /translation="MKIADLMTLLDHHVPFSTAESWDNVGLLIGDGDVEVTGVLTALD
                     CTLEVVNEAIEKGYNTIISHHPLIFKGVTSLKANGYGLIIRKLIQHDINLIAMHTNLD
                     VNPYGVNMMLAKAMGLKNISIINNQQDVYYKVQTYIPKDNVGPFKDKLSENGLAQEGN
                     YEYCFFESEGRGQFKPVGEANPTIGQIDKIEDVDEVKIEFMIDAYQKSRAEQLIKQYH
                     PYETPVFDFIEIKQTSLYGLGVMAEVDNQMTLEDFAADIKSKLNIPSVRFVGESNQKI
                     KRIAIIGGSGIGYEYQAVQQGADVFVTGDIKHHDALDAKIHGVNLIDINHYSEYVMKE
                     GLKTLLMNWFNIEKINIDVEASTINTDPFQYI"
     gene            complement(1107..1784)
                     /locus_tag="PPY92_001019"
     CDS             complement(1107..1784)
                     /locus_tag="PPY92_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HDJ2625037.1"
                     /translation="MISLNNRLTTVSRFLKQGTIADIGSDHAYLPIYAIQNHLCECGI
                     AGEVIQGPFQAAVKNVAANHLVERIDVRLGDGLSVIHPEDVIDNITICGMGGPLIAKI
                     LKDGQDKLSQHPRLILQSNIQTENLRQTLQQLNYEIIDEIIMEEKGHIYEIVVAEHST
                     QLIELSSDELKFGPKLLNNKNEYFIKKWQRELEALYHIKSKLNTEQHHQRLAQINDEI
                     AVIERVL"
     gene            complement(1915..3021)
                     /gene="rpoD"
                     /locus_tag="PPY92_001020"
     CDS             complement(1915..3021)
                     /gene="rpoD"
                     /locus_tag="PPY92_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDJ2625038.1"
                     /translation="MSDNTVKIKKQTIDPTLTLEDVKKQLIEKGKKEGHLSHEEIAEK
                     LQNFDIDSDQMDDFFDQLNDNDISLVNEKDSSDTDEKLNPSDLSAPPGVKINDPVRMY
                     LKEIGRVNLLSAQEEIELAKRIEQGDEVAKSRLAEANLRLVVSIAKRYVGRGMLFLDL
                     IQEGNMGLIKAVEKFDFNKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LIRVQRQLLQDLGRDPAPEEIGEEMDLPAEKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGK
                     VFGVTRERIRQIEAKALRKLRHPSRSKRLKDFMD"
     gene            complement(3245..5062)
                     /locus_tag="PPY92_001021"
     CDS             complement(3245..5062)
                     /locus_tag="PPY92_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDJ2625039.1"
                     /translation="MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEK
                     TPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQS
                     NSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGF
                     APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVG
                     YSGRTYTGQEPKYLNSPETPIFQKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDT
                     AGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATLKTGQHLLQQGLNVF
                     VIQLPSGMDPDEYIGKYGNDAFTTFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKE
                     LSHDISLMKSSILQQKAINDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYDEYG
                     GYIEPEPIGMAQFDNLSRQEKAERAFLKHLMRDKDTFLNYYESVDKDNFTNQHFKYVF
                     EVLHDFYAENDQYNISDAVQYVNSNELRETLISLEQYNLNDEPYENEIDDYVNVINEK
                     GQETIESLNHKLREATRIGDVELQKYYLQQIVAKNKERM"
     gene            complement(5123..5941)
                     /locus_tag="PPY92_001022"
     CDS             complement(5123..5941)
                     /locus_tag="PPY92_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDJ2625040.1"
                     /translation="MEKIKIIVASDSIGETAELVARAGISQFNPKQCKNELLRYPYIE
                     SFEDVDEVIQVAKDTNAIIVYTLIKPEMKQYMSEKVAEFQLKSVDIMGPLMDLLSASV
                     EEKPYNEPGIVHRLDDAYFKKIDAIEFAVKYDDGKDPKGLPKADIVLLGISRTSKTPL
                     SQYLAHKSYKVMNVPIVPEVTPPDGLYDIDPKKCIALKISEEKLNRIRKERLKQLGLG
                     DTARYATEARIQEELNYFEEIVSEIGCPVIDVSQKAIEETANDIIHYIEQNKSK"
     gene            complement(5952..6575)
                     /locus_tag="PPY92_001023"
     CDS             complement(5952..6575)
                     /locus_tag="PPY92_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDJ2625041.1"
                     /translation="MELSQRQERIIEIVKTKGPITGEQIADKLNLTRATLRPDLAILT
                     MSGFLEARPRVGYYYSGKSKGKFFNEKLRQFEVKDYMSQPVVLRENTTVYDAICTIFL
                     EDVSTLFIINEDNDFVGVCSRKDLLRASMIGADIHTVPISVNMTRMPNVTYLEESELV
                     IYAADRMIEKEIDSIPIVRKKDNQKYEVIGRISKTTIAKLLVALYKE"
     gene            6910..8301
                     /locus_tag="PPY92_001024"
     CDS             6910..8301
                     /locus_tag="PPY92_001024"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HDJ2625042.1"
                     /translation="MAKDMDTIVSLAKHRGFVFPGSDIYGGLSNTWDYGPLGVELKNN
                     VKKAWWQKFITQSPFNVGIDAAILMNPKVWEASGHLNNFNDPMIDNKDSKIRYRADKL
                     IEDYMQDVKGDENFIADGLSFEQMKKIIDDEGIVCPVSKTANWTEIRQFNLMFKTFQG
                     VTEDSTNEIFLRPETAQGIFVNYKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFIFRT
                     REFEQMELEFFCKPGEEIEWQNYWKTFASDWLTSLNMSSENMRLRDHDEDELSHYSNA
                     TTDIEYKFPFGWGELWGIASRTDFDLRKHAEHSGEDFRYHDPETNEKYIPYCIEPSLG
                     ADRVTLAFLCDAYDEEGVEGSKDARTVLHFHPALAPYKAAILPLSKKLSGEAIKIFEQ
                     LSSKFSIDFDESQSIGKRYRRQDEIGTPYCVTFDFDSLEDNQVTVRDRDSMEQVRMPI
                     SELEAFLTEKTKF"
     gene            complement(8451..9197)
                     /gene="recO"
                     /locus_tag="PPY92_001025"
     CDS             complement(8451..9197)
                     /gene="recO"
                     /locus_tag="PPY92_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDJ2625043.1"
                     /translation="MRQKGIIIKAVDYGESDKIITILNEHGAKVPLMARRAKKVKTGL
                     QAQTQLFVYGLFIYNQWRGMGTLNSVDVISQHYKLQMDLYVSSYASLAAETIERSMDE
                     GDIAPYNYQLLQFVLEKIESGTSAQLMSVVVMLKCMKRFGFTASFNRCAVSGNDTQAD
                     LIGYSFKFDGAISRQEASKDVHAVILSNKTLYLLDVLQKLPIDKMNSLNIHQEIIDEM
                     SDIILMLYREYAGMFFKSQKLINQLKRLEQ"
     gene            complement(9225..10124)
                     /gene="era"
                     /locus_tag="PPY92_001026"
     CDS             complement(9225..10124)
                     /gene="era"
                     /locus_tag="PPY92_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDJ2625044.1"
                     /translation="MTEHKSGFVSIIGRPNVGKSTFVNRVIGHKIAIMSDKAQTTRNK
                     IQGVMTRDDAQIIFIDTPGIHKPKHKLGDYMMKVAKNTLSEIDAIMFMVNANEEIGRG
                     DEYIIEMLKNVKTPVFLVLNKIDLVHPDELMPKIEEYQSYMDFTEIVPISALEGLNVD
                     HFIDVLKTYLPEGPKYYPDDQISDHPEQFVVGEIIREKILHLTSEEIPHAIGVNVDRM
                     VKESEDRVHIEATIYVERDSQKGIVIGKGGKKLKEVGKRARRDIEMLLGSKVYLELWV
                     KVQRDWRNKVNFIRQIGYVEDQD"
     gene            complement(10125..10529)
                     /gene="cdd"
                     /locus_tag="PPY92_001027"
     CDS             complement(10125..10529)
                     /gene="cdd"
                     /locus_tag="PPY92_001027"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDJ2625045.1"
                     /translation="MSYQPHYFQEVRKAQQESYSPYSQFKVGAYLKTKDGRTFYGTNV
                     ENASYPLSICAERASLVSAISQGYRPGDFESITVTVDADKPSSPCGACRQVLKELCDD
                     DMPVYMTNHKGDMVMMTVAELLPFGFSGKDLE"
     gene            complement(10540..10884)
                     /locus_tag="PPY92_001028"
     CDS             complement(10540..10884)
                     /locus_tag="PPY92_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDJ2625046.1"
                     /translation="MKRFKYALDGLKILIQKDYKFLLHVFAMIVAIVFGLVLNINRIE
                     WIFILIAIALVLTVEALNTAIEYVVDLVTVEYHDLAKYAKDIAAFSVLIVSILAFIIG
                     LIVFLPHFIALF"
     gene            complement(10887..11354)
                     /gene="ybeY"
                     /locus_tag="PPY92_001029"
     CDS             complement(10887..11354)
                     /gene="ybeY"
                     /locus_tag="PPY92_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDJ2625047.1"
                     /translation="MFTIDFSDHTGLVKDAWYKQIEDLLEFAKKEEHIEDDAELSVTF
                     VDKQEIQEINRTYRDKDKVTDVISFALEEDEPEIDFSGLDIPRVLGDIIICTDVAQEQ
                     ANNYGHSFERELGFLALHGFLHLLGYDHMTEADEKEMFGRQDTILNAYGLTRD"
     gene            complement(11355..12302)
                     /locus_tag="PPY92_001030"
     CDS             complement(11355..12302)
                     /locus_tag="PPY92_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDJ2625048.1"
                     /translation="MPGIIQIDDMNQSQALIGNNDEHLKAIEESFDVVIHARGQEVAV
                     KGTKIENVEKAESVLINLLKVIDLGNNITIKDVEAAIKMAHNNTIQHLLDLYDEEITK
                     DAFGKTIRAKTMGQRIYVNAMKNNDLVFGIGPAGTGKTFLAVVYAAKQLRKGAVKRIV
                     LTRPAVEAGESLGFLPGDLKEKVDPYLRPLYDGLYTVLGREQTERFIERGIIEIAPLA
                     YMRGRTLEDAFVILDEAQNTTHAQMKMFLTRLGFGSKMVVTGDQTQIDLPKGVKSGLK
                     EAVSRLHNVKGISILKLDQSDVVRHPLVSKIIEHYEGEN"
     gene            complement(12606..13304)
                     /locus_tag="PPY92_001031"
     CDS             complement(12606..13304)
                     /locus_tag="PPY92_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HDJ2625049.1"
                     /translation="MSVGILIFVISVIISIITTMRENSHKDRQNQKPPQKTSTDNEPK
                     KGGFFEEIERTFKEISEELNEEEKKSSKRKYDDTLPPLFDELPKEEPKSKPVVEPMAP
                     KKQPETKPMTEKPITVPKAEPVEQKHRPSRQDNSDEIRRQLEKSLRDDIKTIRTDIDR
                     EKEKQIAKMEKRARDIIEDKYLSERTKRLKLKQLLNSQNVEKDLTKSAFQFDKDEVIN
                     GMIWSEILAKPKQL"
     gene            complement(13321..14310)
                     /gene="floA"
                     /locus_tag="PPY92_001032"
     CDS             complement(13321..14310)
                     /gene="floA"
                     /locus_tag="PPY92_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500186.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HDJ2625050.1"
                     /translation="MFSLSFIVIAVIIIVALLILFSFVPIGLWISALAAGVHVGIGTL
                     VGMRLRRVSPRKVIAPLIKAHKAGLALTTNQLESHYLAGGNVDRVVDANIAAQRADID
                     LPFERAAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMNGIEVKAKARITVRANIAR
                     LVGGAGEETIIARVGEGIVSTIGSSKHHTEVLENPDNISKTVLSKGLDSGTAFEILSI
                     DIADVDISKNIGADLQTEQALADKNIAQAKAEERRAMAVATEQEMKARVQEMHAKVVE
                     AESEVPLAMAEALRSGNISVKDYYNLKNIEADTGMRNAINKRTDQSDDESPEH"
     gene            complement(14328..15011)
                     /locus_tag="PPY92_001033"
     CDS             complement(14328..15011)
                     /locus_tag="PPY92_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HDJ2625051.1"
                     /translation="MTTILESTAGDTWVEQVSNIIVQPIFTLILTCLTFLGFVYQLYS
                     KKINAAGIIATLSLLILFLGFLIQGNVNMHSILIFSIGVILVVIELFVVGAVIGIIGM
                     ILITISITTLGDNLLFMLANVIVALILTIVEWVILVKIFNRKIPFLDKVILKDSTNSE
                     SGYNSHDNRSHLVGKTAQTVTDLRPAGIIFCENERIDAVSDGNFILRNKTVKILEVEG
                     TRVVVREVD"
BASE COUNT         4669 a         2835 c         2065 g         5492 t
ORIGIN      
        1 agtttaaata tattgaaatg gatctgtatt aattgtagat gcttcaacat ctatatttat
       61 tttttctata ttgaaccaat tcattagtaa cgtttttaaa ccttctttca tcacatattc
      121 gctgtaatga ttaatatcaa ttaaattcac accatgaatt ttagcatcta aggcatcatg
      181 atgtttaata tcacctgtaa caaagacatc tgcgccttgt tggacagctt gatattcata
      241 tccaatacct gaaccaccaa taattgcaat acgtttaatt ttctgattag actcaccaac
      301 aaaacggaca cttgggatat ttaatttaga tttaatatca gctgcgaaat cttccaatgt
      361 catttgatta tccacttctg ccataacgcc aagtccataa agggatgttt gttttatctc
      421 aataaaatca aataccggtg tttcatatgg atggtattgt ttaattaatt gctcagccct
      481 tgacttttga tatgcatcta tcataaattc aattttaact tcatctacat cttcaatttt
      541 atcaatttgt cctattgttg gattagcttc accaactggt ttgaattgcc ctcttccttc
      601 actttcaaag aaacaatatt cataattacc ttcttgcgct aatccatttt cactaagctt
      661 atctttaaat ggtccaacat tatccttagg tatatatgtt tgaactttat agtatacatc
      721 ttgttgatta tttattattg aaatgttctt caaacccatc gccttcgcca acatcatatt
      781 gacaccatac ggatttacat ctaaatttgt atgcatcgct attaaattaa tgtcatgttg
      841 aattagtttt ctaatgatca aaccataacc attagctttt aatgatgtta cgcctttaaa
      901 gattagagga tgatgactaa taatagtatt ataacctttt tcgattgctt catttactac
      961 ttccaacgta cagtctaatg ctgttaaaac accagtaact tcaacatctc catcacctat
     1021 taacaatcct acattatccc aagattcagc agtactaaat ggcacatgat gatctaacaa
     1081 tgtcattaaa tcagctattt tcattactat aacacccttt caattacagc aatttcgtca
     1141 ttaatttgag ctaaacgttg atgatgttgt tcagtattga gtttcgattt aatatgataa
     1201 agtgcttcta actctctttg ccattttttt ataaaatatt catttttgtt gtttagcaat
     1261 tttggtccga atttcaattc atcagatgat agctctatta attgtgtaga atgttctgct
     1321 acaacaattt cataaatatg gcctttttct tccattatta tttcatcaat tatttcataa
     1381 ttcaattgtt gtaatgtttg tcttaaattt tcagtttgta tattactttg taaaatcaac
     1441 cttggatgtt gacttaactt atcttgccca tcttttaaaa ttttagcaat aagtggtccg
     1501 cccataccac aaattgtgat attatcgatt acgtcctcag gatgaataac acttaagcca
     1561 tcccctaaac gtacatcaat tctttctact aaatgatttg cagctacatt tttcacagca
     1621 gcttgaaaag ggccttgaat aacttctcca gcaataccgc attcgcataa atggttttga
     1681 attgcataga ttggcaaata agcatgatct gagccaatat ccgcgattgt accttgtttt
     1741 aaaaatcgac ttaccgtcgt taatcggtta tttaacgaaa tcattttatt ctcctttatg
     1801 aaaaaagagg ttgaacatta ttcaatgtac caacctcact caagttaaat cttaatctaa
     1861 aaataaataa gcgcttattc atgtcttggt atcaatcatt aaaattgata taaattaatc
     1921 cataaagtct ttcaaacgtt tactacgact tggatgtctt aattttctaa gtgcttttgc
     1981 ttcaatttgt cgaatacgtt cacgtgtaac accgaaaact ttaccaactt cttcaagtgt
     2041 tcttgttctg ccgtcatcaa gaccaaatct taatcgtaat acattttctt ctctatcagt
     2101 taatgtatca agcacatctt ctaattgctc ttttaataat tcataagcag catgatctga
     2161 aggactttgt gcttcctgat cctcaataaa gtctcctaaa tgactatcat cttcttcacc
     2221 aattggtgtt tctaatgaaa caggttcttg cgcaattttt aaaatttcac gaactttttc
     2281 tgctggtaaa tccatttctt caccaatttc ttctggtgct ggatctcgac ctaagtcctg
     2341 taataattga cgttgaacac gaattaattt attaattgtt tctaccatat gcacagggat
     2401 acgaatcgta cgtgcttggt cagcaattgc acgagtgatt gcttgtctaa tccaccatgt
     2461 tgcatatgtt gaaaacttaa atcctttgtt aaagtcaaat ttttcaacag ctttaataag
     2521 acccatatta ccttcttgga ttaaatcaag gaataacata ccacgaccta cgtatctttt
     2581 agcaatactt acaactaaac gtaagttcgc ttctgcaagt cttgattttg ctacttcatc
     2641 accttgttca atacgtttgg ctaattcgat ttcttcttgt gcacttaata agttaacacg
     2701 cccaatttct ttaaggtaca tacgaactgg gtcatttatt ttaacacctg gaggggcact
     2761 aagatcactt ggattcagtt tctcgtcagt atctgaacta tctttttcat taactagtga
     2821 aatatcatta tcatttaatt gatcaaagaa atcatccatt tgatcagagt cgatatcaaa
     2881 attctgaagt ttttcagcaa tttcttcatg acttaaatga ccctcttttt tacctttttc
     2941 aattaattgc ttcttaacat cttctaatgt taatgtcgga tcaattgttt gttttttaat
     3001 tttaactgtg ttatcagaca tgaaacggcc tcccgatttt aaatatgaac attcgaaatt
     3061 tattcaatat tgctatttta aactaaattc ttaattaatt catccatatt tttaatttta
     3121 ttttacaaat tggaactaat cccaatattt atttttcaat agtgttgttt aaatgcggta
     3181 actatcattt acccttatac attttgacat aatcattatt cgtattattc tttaaaatca
     3241 catgctacat gcgttcttta ttcttagcaa caatttgctg taaatagtat ttttgtaatt
     3301 ctacatcgcc aatccttgta gcttccctta atttatgatt caatgactca attgtttctt
     3361 gtcctttttc attaataaca ttgacataat catcaatttc attttcatat ggttcgtcat
     3421 tcaaattata ttgttctaag ctaattagtg tttctctcaa ctcatttgaa ttaacatact
     3481 gcacagcatc actgatatta tattgatcat tttccgcata aaaatcatgt aagacttcga
     3541 atacatattt aaaatgctga tttgtgaagt tatccttatc aacactttca taataattta
     3601 aaaatgtatc tttatctctc attaaatgtt ttaaaaatgc tcgctccgct ttttcttgac
     3661 ggctcaaatt gtcaaattgt gccataccaa ttggctcagg ttcaatataa ccgccatact
     3721 catcataacc gccatactca tcttctggat aataattggc tggtgcttga ttgaattgta
     3781 tttcgttagc taactgctca ggactaacat tgaaaaatgg cgcaacatca tttatagcct
     3841 tttgttgcag aattgatgac ttcataagtg aaatgtcatg actcagttct ttcaaataac
     3901 gttcatatga aaggtcatta tgtgcaattt catcttttaa tatacttact ttataatgtg
     3961 caaatgactt tttgtcattt tttacaaaag tagtaaatgc gtcgttgcca tacttaccaa
     4021 tgtattcatc cggatccatg ccagatggca attgtataac aaatacattt agcccttgct
     4081 gtaacaaatg ttgacctgtt ttaagtgttg cttcactacc cgcaaaatcc ccatcaaaca
     4141 ttaatgttat atttgatgtt aactttcgta taaaggtaat atgttcatct gacaactgtg
     4201 tacccattgt tgcaacaacg tttttcaagc cagcagtatc agattttata acatccataa
     4261 aaccttctag taatacaatt tcatctaatt ttctaattga tttacgtgct ttatctaagt
     4321 tatataacaa ctttcttttt tgaaagatag gcgtttcagg actatttagg tattttggtt
     4381 cttgaccggt atatgttcga cctgaatatc caacaattct tccttgcgca tttttcaaag
     4441 gaaacataat acgatttcga aatctatcgt aataactgaa attttcttcg ttacgtgata
     4501 ataatccggc ttcatatgct aattcaatat cgtaaccctt tttttgaaga aaatcatgac
     4561 aaaaatgtga gctatcgggt gcaaagccaa tgcctcgctc tttaataagc gcatctgtaa
     4621 aaccacgttc ttgtaagtat gttaatgctt gttcgccttc gactgtcttt gttaaagcgt
     4681 aataataaaa ttcttgtatt aactcatgca tttcaatcat ttgtaaatca tcagaagcaa
     4741 tttgaacatt tgagttagat tgtgttgcct caatatctac agcaacatta actctatcac
     4801 ctaattcttt aaccgcttca acaaatgata tgtctttaat ttcttgagta aattgaaaaa
     4861 cattgccacc ttttttacaa ccaaaacaat gacagatttg tttatcttca gaaactgtaa
     4921 atgaaggtgt cttttcatca tgaaaaggac acaaacctat ataattgcgt cctctctttt
     4981 ctagttttac atattcactt accaagtcta aaatgtcggt tttatctttt atttcattaa
     5041 tgatcgattg atctattcgc aaattaaatc acctattaag cattagttat actattatac
     5101 ctaatttttg acaaaaatga aatcatttcg atttattttg ttcaatataa tggattatat
     5161 cgtttgctgt ttcttcgatt gctttttgag aaacatcaat gacaggacat ccaatttcac
     5221 ttacgatttc ttcaaagtaa ttcaattctt cttggattcg agcttctgtt gcataacgag
     5281 ctgtgtcacc tagtcccaat tgttttaatc gctcttttct aatgcgattt aatttttctt
     5341 cacttatttt aagtgcgata catttcttag gatcaatatc atataagcca tctggcggtg
     5401 tcacttctgg tacaatcggt acattcataa ctttgtaact cttatgcgct aaatactgag
     5461 ataatggtgt ctttgaagtt ctcgaaatac caagtaaaac aatatcagct ttaggtaatc
     5521 ctttaggatc tttaccatca tcatatttaa ccgcaaactc tatcgcatca attttcttga
     5581 aatatgcatc atctaatcta tgaacgatac ctggctcatt ataaggtttt tcttcaaccg
     5641 atgctgataa taaatccatt aatggcccca tgatatcaac ggacttcaat tggaattctg
     5701 ctactttctc actcatatat tgcttcattt caggtttaat aagtgtataa acaatgatag
     5761 catttgtatc ttttgcaact tgaatcactt catcaacatc ttcaaaagat tcaatatatg
     5821 gatatcttaa taattcattt ttacattgct taggattaaa ttgtgaaata cctgccctag
     5881 caactaactc tgccgtttca cctatagaat ctgaggctac gataatttta attttttcca
     5941 ttacttctca cctattcttt atataatgct actaataact tagctattgt tgttttggaa
     6001 attcttccaa ttacttcata cttttgatta tctttttttc ttacaattgg aatcgaatca
     6061 atttcttttt caatcattct atctgctgcg tatatgacta attcgctttc ctctaaataa
     6121 gtgacattag gcatacgtgt catatttaca ctgataggta ctgtatgaat atctgctcca
     6181 atcattgaag ctcttaataa atcttttctt gaacacacac caacaaaatc gttatcttca
     6241 ttaataataa ataatgtact tacatcttct aaaaaaattg tacaaatagc gtcataaact
     6301 gttgtattct ctcttagcac aacaggttga gacatatagt ccttaacctc aaattgtcga
     6361 agtttttcat taaaaaattt gccttttgat ttacctgaat aataatatcc aactcgggga
     6421 cgcgcttcta aaaaacctga cattgttaat atcgctaaat ctggtctaag cgttgctctt
     6481 gttaaattca acttatctgc tatttgttca ccagtaatgg gtcctttagt tttaacaatt
     6541 tcgatgattc gttcttgtct ttgactgagt tctataggtc ttcacccctt ttttattcac
     6601 ttattattat accgatattt ctttcatgtc acaaacacat taattttact tgcctttaaa
     6661 taatctatca attagaataa acgtacagcg agttaaggat agtgtaagct taacaataag
     6721 attggcgcaa cgaatcattt taaaataaaa gcgagtgact acactaattt gggtggaacc
     6781 gcgggttaaa tacgtaactt attttgtatt atatacaatt taaaatatta actcgtccca
     6841 tggaagtaaa gcatatttac tatgctgtta ttttccatgg ggcgttttca tgtatgagga
     6901 gaggtaatta tggcaaaaga tatggataca attgtttcat tagcaaaaca cagaggtttt
     6961 gtgttccctg gtagtgatat ttacggtggt ttatcaaaca catgggatta tggtccttta
     7021 ggtgttgaat taaagaataa tgttaaaaaa gcttggtggc aaaaattcat tacacaatca
     7081 ccgtttaacg ttggtatcga cgctgcaatc ttaatgaatc caaaagtatg ggaagcttca
     7141 ggacacttaa acaacttcaa cgacccaatg attgataata aagatagtaa aattcgatat
     7201 cgcgctgata aattaattga agattatatg caagatgtta aaggtgatga aaacttcatt
     7261 gccgatggtt taagttttga acaaatgaaa aaaattattg acgatgaagg tattgtttgt
     7321 cctgtaagta aaactgctaa ctggactgaa attcgccaat tcaatttaat gtttaaaaca
     7381 ttccaaggtg taactgaaga ttctacaaat gaaattttct tacgtcctga aacagcacaa
     7441 ggtatttttg taaactataa aaacgtgcaa cgttcaatgc gtaaaaaatt accatttggt
     7501 atcggtcaaa ttggtaaatc attccgtaat gaaatcactc caggtaactt cattttcaga
     7561 acaagagaat ttgaacaaat ggaacttgaa ttcttctgta aacctggaga agaaatcgaa
     7621 tggcaaaatt attggaaaac ttttgcaagt gactggttaa caagcttaaa tatgagcagt
     7681 gaaaatatgc gtttacgtga tcatgatgaa gatgaattat ctcattactc aaatgcaaca
     7741 actgatattg aatataaatt cccatttggt tggggtgagt tatggggtat cgcaagtcgt
     7801 acagacttcg acttacgtaa acatgctgaa cactctggtg aagatttcag ataccatgat
     7861 ccagaaacga acgaaaaata tattccatat tgtatcgagc catcacttgg tgcagatcgt
     7921 gtaacattag ctttcttatg tgatgcatat gatgaagaag gcgttgaagg tagtaaagat
     7981 gcacgtacag ttttacactt ccatcctgca ttagcaccat ataaagcagc gattttacct
     8041 ttaagtaaga aattatctgg cgaagcgatt aagatttttg agcaattaag ttctaaattc
     8101 tcaatcgatt tcgatgaatc acaatctatc ggtaaaagat accgtcgtca agatgaaatc
     8161 ggtacacctt attgtgtaac attcgacttt gattcattag aagataatca agttacagta
     8221 cgtgacagag attcaatgga acaagttcgt atgccaatct cagagttaga agctttctta
     8281 actgaaaaaa caaaattcta attaaaaaga ctttccacca tatgtggtgg ctatataatt
     8341 tataaaaaga gatactggtt agtaaaattg ccagtatctc ttttctattc aaaaaggtat
     8401 tcaaataaac acatctatgt ggctacttga atacctttta ttattttatg ttattgttcc
     8461 aatcttttta attggttgat tagtttctga cttttaaaaa acatacctgc atattcacga
     8521 tataacatta aaatgatatc tgacatttca tcaataattt cttgatggat attcaatgaa
     8581 ttcattttat ctatcggtaa tttttgtaat acatctaata aatatagtgt tttattcgat
     8641 aatataactg catgtacatc tttagaagcc tcttgccttg aaatcgcacc gtcaaactta
     8701 aaactataac ctattaaatc tgcttgtgtg tcattaccac tcacagcaca gcgattaaat
     8761 gatgcagtaa aaccaaatcg tttcatgcac tttaacataa ctacgactga cattaactgt
     8821 gcagatgtac ctgattctat tttttcaaga acaaattgta ataattgata gttatatggt
     8881 gcaatgtcac cttcatccat tgagcgctca atagtttcag ctgccagaga ggcataactg
     8941 cttacgtaaa ggtccatttg taatttataa tgttgactaa taacatctac agaatttaac
     9001 gttcccatac ctcgccactg attgtaaata aacaaaccat aaacaaacaa ttgcgtttgc
     9061 gcttgtaaac ccgtcttaac ttttttagca cgccttgcca taagtggtac ttttgcacca
     9121 tgctcattta aaatcgtgat aattttatca gattcaccat aatcaactgc tttgatgata
     9181 atcccttttt ggcgcattaa caattatctt caccactttt aagattaatc ttggtcttca
     9241 acataaccaa tttggcgaat aaagttaact ttgtttcgcc agtctctttg aactttgacc
     9301 cataattcta agtatacttt agagcctaga agcatttcta tatcacgtct cgcacgtttt
     9361 cctacttctt ttaacttttt accgcctttt ccaatgacaa ttcctttttg cgaatctctt
     9421 tcaacatata tagttgcttc gatatgaaca cgatcttcgc tttctttaac catacggtcc
     9481 acattaacac caatcgcatg agggatttct tcacttgtaa gatgaaggat tttttcacga
     9541 atgatttcac ccactacaaa ttgttcagga tggtctgaaa tttgatcatc tggataatat
     9601 ttaggtcctt cgggtaaata cgtctttaaa acatcaataa aatgatctac atttagccct
     9661 tctaatgctg aaataggtac aatctctgta aagtccatat aactttgata ttcttcaatc
     9721 tttggcatta attcatctgg atgcactaaa tctattttat ttaatactaa aaatactggt
     9781 gtcttaacat ttttcaacat ttctataata tattcatcgc ctcgtccaat ttcctcattg
     9841 gcattaacca taaacatgat tgcatctatt tcagataatg tatttttagc gactttcatc
     9901 atatagtcac ctaatttgtg tttaggttta tgaatacctg gcgtatcaat gaatataatt
     9961 tgcgcgtcat ctcttgtcat aacaccttga attttatttc tagttgtttg agctttatcg
    10021 gacatgattg ctattttatg gccaatcact ctattaacaa atgttgactt ccctacattt
    10081 ggtctaccta taattgaaac aaatcctgat ttatgttctg tcatttattc taaatccttt
    10141 cctgaaaatc caaatggtag taactctgcg actgtcatca taaccatatc tcctttatga
    10201 tttgtcatat acacaggcat atcatcatca cataattcct ttaaaacttg acgacatgca
    10261 ccacaaggtg atgacggttt atctgcatct acggttacag ttattgattc aaaatcacct
    10321 ggtctgtatc cttgagaaat tgccgatacc aaactagctc gttcagcaca tatcgataat
    10381 ggataagaag cattttctac attggtacca taaaaagttc taccgtcttt cgtttttaaa
    10441 taagccccta ctttaaattg actgtatggc gaatatgatt cttgttgtgc ttttctaact
    10501 tcttgaaaat aatgaggttg ataactcata tatgcctccc taaaataacg ctataaaatg
    10561 tggtaaaaat actattaaac ctataataaa tgctaatatt gaaactataa gtacactaaa
    10621 agccgctata tcttttgcgt atttagctaa atcatgatat tcaacggtca ctaaatcgac
    10681 aacatattca atagcagtgt ttaaagcttc aacagtgaga actaatgcaa tagcaatgag
    10741 tataaatatc cactcaatac gattaatatt tagtacgaga ccaaagacaa tagcaacaat
    10801 cattgcaaac acatgtaaaa gaaatttata gtctttttga attaagattt tcagcccatc
    10861 aagtgcatat ttaaaccttt tcataattag tctcgtgtta atccataagc gtttaatatt
    10921 gtatcttgtc gaccaaacat ttccttttca tccgcttcag tcatatgatc ataacctaat
    10981 agatgcaaaa atccatgtaa tgctaaaaat cctaattctc gttcaaaaga atgtccgtaa
    11041 ttgtttgctt gttcttgcgc tacatccgta cagataatta tatcccctaa aacacgtggt
    11101 atatcaagac cactaaaatc aatctctggc tcatcttctt ctaaagcaaa tgagattaca
    11161 tctgtaactt tatctttatc tctatatgtt cgattaattt cttgtatttc ttgtttatct
    11221 acaaatgtaa cagaaagctc agcatcgtct tctatatgct cttctttttt agcaaattct
    11281 aataaatctt caatttgttt ataccacgca tctttaacta agcctgtgtg atcgctaaaa
    11341 tctatcgtaa acatttaatt ctctccttca taatgttcaa tgatcttact taccaatgga
    11401 tgtcttacta catcgctctg atctaatttc aatatactta tacctttaac gttgtgtaac
    11461 ctactgaccg cttccttaag tccactttta acacctttag gtaaatcgat ttgagtttgg
    11521 tcaccagtaa ctaccatttt tgagccaaaa cctagtcttg ttaaaaacat tttcatttgc
    11581 gcatgtgtcg tattctgcgc ctcatcaaga attacaaatg catcttctaa tgttcgtccg
    11641 cgcatatatg caagtggcgc tatttcgata atgcctcttt caataaatcg ctctgtttgt
    11701 tcacgcccaa gaacagtata tagaccatca tataaaggtc ttaaatatgg atctaccttt
    11761 tctttcaaat ctcctggtaa aaatccaagt gactctcctg cttcaacagc aggtcttgtt
    11821 aatacaatac gtttaacagc acctttacgg agttgctttg ctgcataaac tacagctaag
    11881 aatgtcttac ctgtaccagc aggacctata ccaaatacta aatcattatt tttcatggca
    11941 ttaacatata tacgttgccc catcgttttc gcacgaatcg tcttaccaaa tgcatcttta
    12001 gttatctctt catcatataa atctaacaga tgttgaattg tgttattatg cgccatttta
    12061 atagctgctt caacatcttt aattgtaata ttattaccta aatcaataac cttcagcaaa
    12121 ttgattaata ctgattccgc tttttctacg ttttctattt ttgtaccttt aacggcaact
    12181 tcttgtcctc ttgcatggat gacaacatcg aaactctctt caattgcttt taaatgttca
    12241 tcattatttc caattaaagc ttgagattgg ttcatatcgt ctatttgtat aattccaggc
    12301 atacacgcgc tccttttcat atatcattta tattcattct atgatgcttt aatatctact
    12361 taattatatc atgcaattga ttgatttaaa catctttgca ttgacttact catttttctt
    12421 taatttaaaa taatcattta tacttttcac cttgtcataa aagcagtttc aattttagac
    12481 taatcaattt tttgctgtaa tttaaataac taatatttct cgaaagattc tctttgtttt
    12541 ttaatttttc ttaacgtaaa aaaacaagca actaccatta cgatagtgct tgttttcaaa
    12601 aaattttata attgttttgg tttagctaaa atttctgacc atatcatacc attgattact
    12661 tcatctttat caaattggaa cgctgattta gtcaaatctt tttcgacatt ttgagaatta
    12721 agcaactgct ttaatttcaa acgttttgta cgttcagata agtatttatc ctcaataata
    12781 tctctagcac gtttttccat tttagcaatt tgcttttctt tttctctatc aatgtcagta
    12841 cgaatcgttt taatatcatc tctaagtgat ttttctaatt gacgtctaat ttcgtcagaa
    12901 ttatcttgtc tagaaggtct atgtttctgc tccaccggtt ctgctttagg cactgtaatt
    12961 ggtttctctg tcatcggttt tgtttctggt tgttttttag gtgccatagg ttctacaaca
    13021 ggtttcgatt taggctcttc ctttggaagt tcatcgaata aaggtggtaa cgtatcatca
    13081 tattttcgtt tcgatgattt cttttcttct tcatttaatt cttcacttat ttctttaaac
    13141 gttcgctcaa tttcttcaaa aaagccacct ttttttggtt cattatcggt agatgttttt
    13201 tgaggtggct tttgattttg tctatcttta tgactatttt cgcgcatagt agtaatgata
    13261 gaaatgatca ctgatatgac aaaaattaga ataccgacac tcatttaatc acctctcgac
    13321 ttaatgttca ggtgactcat catcactttg atcagttcgt ttattaattg catttctcat
    13381 gcctgtatca gcttcgatat ttttcaaatt ataataatct ttaacactga tattacctga
    13441 acgtaatgct tcagccatag ctaatggtac ttcagattcg gcttcaacta ctttagcatg
    13501 catttcttgt acacgcgctt tcatttcttg ctcagttgct acagccatag ctctacgttc
    13561 ttcagctttt gcttgtgcaa tatttttgtc tgctaatgct tgttcagttt gtaagtctgc
    13621 accaatattt ttactaatat caacgtcagc aatatcaatt gataaaattt caaatgcagt
    13681 acctgaatct aaacctttgc ttaaaactgt tttagaaata ttatctgggt tttcaagtac
    13741 ttctgtatga tgcttactag aaccaattgt tgaaacgata ccttcaccaa cacgtgcgat
    13801 gattgtttct tcacctgcac caccaacaag tcgagcaata ttagctctaa ctgtgatacg
    13861 agctttggct ttcacttcaa taccgttcat tgctacacct gcgataaatg gtgtttcaat
    13921 gactttagga ttaacagaca tttgaaccgc ttctaatacg tcacgtcctg caaggtcaat
    13981 tgcagcagca cgttcgaaag gaagatcaat gtcagcacgt tgtgcagcaa tattagcatc
    14041 aacaactctg tcaacatttc ctcctgctag ataatgcgat tctaattggt ttgttgttaa
    14101 tgctagtcct gctttgtgcg ctttaattaa tggcgctata acttttcttg gagatacacg
    14161 acgtaaacgc ataccaacca atgtacctat accaacatga acgccagctg ctaacgctga
    14221 aatccacaaa ccaatcggta caaatgagaa taaaataagt aatgcaacta ttataataac
    14281 tgctattacg ataaaactta aactaaacat ggtatcgctc cttttaatta atctacttcc
    14341 ctcacaacta ctcttgttcc ttcaacttca aggattttca ccgttttatt gcgtaaaata
    14401 aagttgccat ctgaaacagc atcaatacgt tcattttcac aaaaaataat ccctgcaggt
    14461 cgaagatctg taactgtttg agcagtcttt cctacgaggt gcgagcggtt atcatgagaa
    14521 ttgtaacctg actcagaatt agttgaatct tttaagataa ccttatccaa aaacggaatc
    14581 tttctgttga aaatcttcac taatatcacc cattctacaa tcgttaaaat caaggcaacg
    14641 ataacattcg caagcataaa tagcaaatta tcaccgagcg ttgttatgct tatagttatc
    14701 agtatcatgc caataatacc aattactgca ccaactacaa ataattcaat tacaactaat
    14761 ataacgccaa ttgagaatat taagatagaa tgcatattga catttccttg gattagaaat
    14821 cccaaaaata aaataagtaa tgataatgtg gcgataatac cagctgcatt gattttttta
    14881 gagtaaagtt gatatacaaa tcctaagaat gtcaaacagg ttaatattaa cgtaaaaata
    14941 ggttgaacaa ttatattact aacttgttca acccaagtat ctccagccgt tgattccaag
    15001 atagttgtca tttgtaaaaa attattataa ctcacaattt cacctcgccc tcacattaat
    15061 t
//
LOCUS       DANBWH010000049        14876 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.051, whole genome shotgun sequence.
ACCESSION   DANBWH010000049 DANBWH010000000
VERSION     DANBWH010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14876
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.051"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..109
                     /locus_tag="PPY92_001034"
     CDS             <1..109
                     /locus_tag="PPY92_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="staphostatin A"
                     /protein_id="HDJ2625052.1"
                     /translation="VIEIFIPNDNKILLTIMNTEALGTSPRMTFIKHKS"
     gene            complement(401..574)
                     /locus_tag="PPY92_001035"
     CDS             complement(401..574)
                     /locus_tag="PPY92_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NETI motif-containing protein"
                     /protein_id="HDJ2625053.1"
                     /translation="MKFKVNENETIADCLSRMKMQGYMPVKRIEKPVFQEQKDGTVEV
                     SHQEIVFVGKKIQ"
     gene            complement(555..1157)
                     /locus_tag="PPY92_001036"
     CDS             complement(555..1157)
                     /locus_tag="PPY92_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HDJ2625054.1"
                     /translation="MSFVTENPWLMVLTIFIINVCYVTFLTMRTILTLKGYRYIAASV
                     SFLEVLVYIVGLGLVMSNLDHIQNIIAYAFGFSIGIIVGMKIEEKLALGYTVVNVTSA
                     EYELDLPNELRNLGYGVTHYAAFGRDGSRMVMQILTPRKYERKLMDTIKNLDPKAFII
                     AYEPRNIHGGFWTKGIRRRKLKDYEPEELESVVEHEIQSK"
     gene            complement(1428..2249)
                     /gene="nadE"
                     /locus_tag="PPY92_001037"
     CDS             complement(1428..2249)
                     /gene="nadE"
                     /locus_tag="PPY92_001037"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDJ2625055.1"
                     /translation="MSKLQDVIVQEMKVKKRIDSAEEIMELKQFIKNYVQSHSFIKSL
                     VLGISGGQDSTLVGKLVQMSVNELREEGIDCTFIAVKLPYGVQKDADEVDQALRFIEP
                     DEIVTVNIKPAVDQSVQSLKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTD
                     HSAENITGFYTKYGDGAADIAPIFGLNKRQGRQLLAYLGAPKELYEKTPTADLEDDKP
                     QLPDEDALGVTYEAIDNYLEGKPVTPEEQKVIENHYIRNAHKRELAYTRYTWPKS"
     gene            complement(2242..3711)
                     /locus_tag="PPY92_001038"
     CDS             complement(2242..3711)
                     /locus_tag="PPY92_001038"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDJ2625056.1"
                     /translation="MYQLEDDSLMLHNDLYQINMAESYWNDNIHEKMAVFDLYFRKMP
                     FNSGYAVFNGLKRVIDFIEHFGFSESDLEYLKSIGYKDDFLSYLKDLKFTGSIRSMQE
                     GELCFGNEPLLRVEAPLIQAQLIETILLNIVNFHTLITTKASRIRQIASNDKLMEFGT
                     RRAQEIDAALWGARAAYIGGFDSTSNVRAGKLFGIPVSGTHAHAFVQTYGDEYVAFKK
                     YAERHKNCVFLVDTFHTLKSGVPNAIKVAKELGDKINFVGIRLDSGDIAYLSKEARRM
                     LDEAGFTETKIIASNDLDEETITSLKAQGAKVDSWGVGTKLITGYDQPALGAVYKLVA
                     IENEEGSYSDRIKLSNNAEKVTTPGKKNVYRIINKKTGKAEGDYITLENENPYDEQPL
                     KLFHPVHTYKMKFIKSFEAIDLHHNIYENGKLVYQMPTEDESREYLAQGLQSIWDENK
                     RFLNPQEYPVDLSKACWDNKHKRIFEVAEHVKEMEEDNE"
     gene            3895..4971
                     /locus_tag="PPY92_001039"
     CDS             3895..4971
                     /locus_tag="PPY92_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase oxygenase"
                     /protein_id="HDJ2625057.1"
                     /translation="MLFKEAQAFIENMYKECHYETQIINKRLHDIELEIKETGTYTHT
                     EEELIYGAKMAWRNSNRCIGRLFWDSLNVIDARDVTDEASFLSSITYHITQATNEGKL
                     KPYITIYAPKDGPKIFNNQLIRYAGYDNCGDPAEKEVTRLANHLGWKGKGTNFDVLPL
                     IYQLPNESVKFYEYPTSLIKEVPIEHNHYPKLRKLNLKWYAVPIISNMDLKIGGIVYP
                     TAPFNGWYMVTEIGVRNFIDDYRYNLLEKVADAFEFDTLKNNSFNKDRALVELNYAVY
                     HSFKKEGVSIVDHLTAAKQFELFERNEAQQGRQVTGKWSWLAPPLSPTLTSNYHHGYD
                     NTVKDPNFFYKKKESNANQCPFHH"
     gene            4991..5785
                     /locus_tag="PPY92_001040"
     CDS             4991..5785
                     /locus_tag="PPY92_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDJ2625058.1"
                     /translation="MQLYYLGPKGTFSYLACRQYFSENEATFQPKSNLFEVIKAVADD
                     DTSIGVVPIENSIEGTINIVADALAQQDVFAHGEIRLDINFALYGNGTDSISDIKKVY
                     SIAPAISQTTNYIHQHQFDYDYVDSTIQSLTKIENGVAAIAPLGSGEAYGFTPIDTHI
                     EDYPHNVTRFLVIKNQQQFDQNATSLMFLITPMHDKPGLLASVLNTFALFNINLSWIE
                     SRPLKTQLGMYRFFVQADSAITTDIKKVIAILETLDFKVEMIGAFN"
     gene            5857..5964
                     /locus_tag="PPY92_001041"
     CDS             5857..5964
                     /locus_tag="PPY92_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625059.1"
                     /translation="MVDFLLFVLFLHDAAVYVSLPLVQAIANVTFYFSF"
     gene            complement(5975..7537)
                     /locus_tag="PPY92_001042"
     CDS             complement(5975..7537)
                     /locus_tag="PPY92_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDJ2625060.1"
                     /translation="MAYFNQHQSMISKRYLTFFSKSKKKKPFSAGQLIGLILGPLLFL
                     LTLLFFHPQDLPWKGVYVLAITLWIATWWITEAIPIAATSLLPIVLLPLGHILTPEQV
                     SSEYGNDIIFLFLGGFILAIAMERWNLHTRVALTIINLIGASTSKILLGFMVATGFLS
                     MFVSNTAAVMIMIPIGLAIIKEAHDLQEANTNQTSIQKFEKSLVLAIGYAGTIGGLGT
                     LIGTPPLIILKGQYMQHFGHEISFAKWMIVGIPTVIVLLGITWLYLRYVAFRHDLKYL
                     PGGQTLIKQKLDELGKMKYEEKVVQTIFVLASLLWITREFLLKKWEVTSSVADGTIAI
                     FISILLFIIPAKNTEKHRRIIDWEVAKELPWGVLILFGGGLALAKGISESGLAKWLGE
                     QLKSLNGVSPILIVIVITIFVLFLTEVTSNTATATMILPILATLSVAVGVHPLLLMAP
                     AAMAANCAYMLPVGTPPNAIIFGSGKISIKQMASVGFWVNLISAIIIILVVYYVMPIV
                     LGIDINQPLPLK"
     gene            complement(7743..8840)
                     /locus_tag="PPY92_001043"
     CDS             complement(7743..8840)
                     /locus_tag="PPY92_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HDJ2625061.1"
                     /translation="MYINMKDYGLTGINKTKDTRAIQRALNRGRCKPTTVYIPKGTYD
                     ICKPLTIYGNTTLLLDNETILRRCHSGPLLKNGHRFGFYRGYNGHSHIHIKGGKFDMN
                     GVSYPYNNTAMCIGHAEDIQLIGVTIKNVVSGHAIDACGINGLYIKSCSFEGFIDYSG
                     ERFYSEAIQLDIQVPGAFPKFGTTDGTITKNVVIEGCYFGPSELPEMGSWNRAIGSHA
                     SRHNRYYENIHIRNNIFEDIQGYALTPLKYKDAFIINNKFINCEGGIRYLGVRDGKNA
                     ADVMTGKDLGSQAGINMNIIGNEFKGSMSKDAIHVRNYNNVKHKDVLIVGNTFNNSTQ
                     SIHLEDIDTVFLSPVEAGIQVTTINIDEIKK"
     gene            9209..9769
                     /locus_tag="PPY92_001044"
     CDS             9209..9769
                     /locus_tag="PPY92_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDJ2625062.1"
                     /translation="MTHRALLVVDYSYDFIADDGLLTCGKPGQNIEDFIVSRINDFNY
                     YQDHIFFLMDLHYLHDIHHPESKLFPPHNIVDTSGRELYGKVGKLYETIKAQPNVHFI
                     DKTRYDSFFGTPLDSLLRERSINQVEIVGVCTDICVLHTAISAYNLGYKISVPAEGVA
                     SFNQKGHEWALAHFKNSLGAEVEQHV"
     gene            9822..10751
                     /locus_tag="PPY92_001045"
     CDS             9822..10751
                     /locus_tag="PPY92_001045"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HDJ2625063.1"
                     /translation="MAKTYIFGHKNPDTDAISSAIIMAEFEQLRGNSGAKAYRLGDVS
                     AETQFALDTFNVPAPELLTDDLDGQDVILVDHNEFQQSSDTIASATIKHVIDHHRIAN
                     FETAGPLCYRAEPVGCTATILYKMFRERGFEIKPEIAGLMLSAIISDSLLFKSPTCTQ
                     QDVKAAEELKDIAKVDIQKYGLDMLKAGASTTDKSVEFLLNMDAKSFTMGDYVTRIAQ
                     VNAVNLDEVLNRKEDLEKEMLAVSAQEKYDLFVLVVTDIINSDSKILVVGAEKDKVGE
                     AFNVQLEDDMAFLSGVVSRKKQIVPQITEALTK"
     gene            complement(10944..11117)
                     /locus_tag="PPY92_001046"
     CDS             complement(10944..11117)
                     /locus_tag="PPY92_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625064.1"
                     /translation="MIVVAVNFKWSFELYMLKVENRAMNRVHNNIYNFNHNEKGRKKT
                     KIYTQHRKYFKKM"
     gene            11169..12548
                     /locus_tag="PPY92_001047"
     CDS             11169..12548
                     /locus_tag="PPY92_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDJ2625065.1"
                     /translation="MNIIEQKFYDSKAFFNTQQTKDISFRKDQLKKLSKAIKSYESDI
                     LEALYTDLGKNKVEAYATEIGITLKSIKNARKELKNWTKTKNVDTPLYLFPTKSYIKK
                     EPYGTVLIIAPFNYPFQLVFEPLIGAIAAGNTAIIKPSELTPNVARVIKRLINETFDA
                     NYIEVIEGGIEETQTLIHLPFDYVFFTGSENVGKIVYQAASENLVPVTLEMGGKSPVI
                     VDETANIKVASERICFGKFTNAGQTCVAPDYILVHESVKDDLITALSKTLREFYGQNI
                     QQSPDYGRIVNLKHYHRLTSLLNSAQMNIVFGGHSDEDERYIEPTLLDHVTSDSAIMQ
                     EEIFGPILPILTYQSLDEAIAFIHQRPKPLSLYLFSEDENATQRVINELSFGGGAIND
                     TLMHLANPKLPFGGVGASGMGRYHGKYSFDTFTHEKSYIFKSTRLESGVHLPPYKGKF
                     KYIKAFFKN"
     gene            complement(12668..13696)
                     /locus_tag="PPY92_001048"
     CDS             complement(12668..13696)
                     /locus_tag="PPY92_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HDJ2625066.1"
                     /translation="MTNGYIGSYTKKNGKGIYRFELNENQSRIDLLEIGFELEASTYL
                     VRNNEVLYGINKEGEQCGVASLKIDDNGELHLLNKCLSSKAGTGCYVSISEDKRYLFE
                     AVYGAGIIRMYELNTHTGEIIRLIQELAHDFPTGTHERQDHPHAHYINQTPDGKYVAV
                     TDLGADRIVTYKFDDNGFEFYKESLFKDSDGTRHIEFHDNGKFAYVVHELSNTVSVAE
                     YNDGKFEELERHLTIPENFDGDTKLAAVRLSHDQQFLYVSNRGHDSIAIFKVLDNGQH
                     LELVTITESGGQFPRDFNIASSDDLLVCAHEQGDSVVTVFERSKETGKITLCDNTRVA
                     SEGVCVIF"
     gene            13966..14367
                     /locus_tag="PPY92_001049"
     CDS             13966..14367
                     /locus_tag="PPY92_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HDJ2625067.1"
                     /translation="MTSYEEETDYRNVPRELLDNNIPMGRGMIKWAPFATLPEQFETI
                     QQYIIDQNKITRPVLSDDQLAELNIRLHEALQYAQPVEVKFYNNGFVDSVRLTIYRID
                     AINYEIDGYVYNQQQRQKISIFDILDIVLLP"
     gene            complement(14385..14593)
                     /locus_tag="PPY92_001050"
                     /pseudo
     CDS             complement(14385..14593)
                     /locus_tag="PPY92_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4964 a         2631 c         2188 g         5093 t
ORIGIN      
        1 tgttattgaa atatttatac caaatgataa taaaatacta ctaacaatta tgaacacaga
       61 agctttggga acttctccta gaatgacttt cattaagcat aagtcataag actatctaga
      121 tatgattaag taaactatta atcttattaa aggtctctag gaagaaaaaa tttcatgcga
      181 tgagattgtt ttagaaaaag cgacgatata tataatctaa caataatttt taaaatatta
      241 agaaccccca gcatatgctt tatatagctt gctgggggtt tctcatacaa tatggatcga
      301 gcatcttgca tgtataaaca aataggctat tcaaagtaaa attactcaaa ctttgaatag
      361 cctttcctct tcaaatagtc tttatttaaa ttggattatg ttattggatt ttcttaccta
      421 caaaaacgat ttcttgatgt gatacttcaa ccgtgccatc tttttgctct tgaaacacgg
      481 gtttttcaat acgttttacc ggcatatacc cttgcatttt cattcgtgac aaacaatcag
      541 caattgtttc attctcattt actttgaatt tcatgttcta ctacactttc cagttcttct
      601 ggttcataat ctttaagctt tctacgacga atgcctttag tccagaatcc accatgtatg
      661 tttcgaggtt catacgcaat gataaatgct ttcggatcta aattttttat cgtatccatc
      721 aatttacgtt catattttct tggtgttaaa atttgcatca ccatacgact accatctcta
      781 ccaaacgcag catagtgcgt aacgccatat cctaaatttc gaagttcatt cggtaaatct
      841 aactcatatt ctgctgaagt tacatttaca actgtataac ctaatgccag tttttcttct
      901 attttcatac caacaatgat acctattgaa aaaccaaatg cgtaggcaat aatattttga
      961 atatggtcta aattagacat aaccaaacct aaaccaacga tataaactaa tacttctaaa
     1021 aaactaactg atgcagcaat ataacgataa cctttcaacg ttaaaattgt tcgcatcgtt
     1081 aaaaacgtta cataacaaac gttaatgata aatatagtta gtaccattaa ccatggattt
     1141 tctgttacga atgacataag gcgccccact ctctttcaat tacactttaa ataccaaact
     1201 gcttcaacct aaccttaatg aatgcatatt aatataatat agtacgaaaa ctctcataaa
     1261 taaatagcga atttgtcata aataaaaatt tattctcaaa tgaacatact tactttagtt
     1321 catacacttt caatttatac actgaagtag tctagctgaa tgtggtttaa tgttattaat
     1381 tctaactcat atttaattta agtcacacgt tagaagaaaa aattaaatta ggattttggc
     1441 cacgtgtatc ttgtatatgc aagttcacgt ttgtgtgcat ttcgtatata atgattttca
     1501 attacttttt gttcttctgg cgtaactggc ttaccttcta aataattatc aatcgcctca
     1561 taagttacac ctaatgcatc ttcatctgga agctgtggtt tatcatcttc taaatcagca
     1621 gttggcgttt tttcatataa ttcctttggc gcaccaagat acgctaataa ttgacgacct
     1681 tgtcgtttat tcaaaccaaa tataggtgcg atatctgcag caccatcacc gtacttcgta
     1741 taaaacccag ttatattttc agctgaatga tctgttccta ctacaatacc ttgtcggttt
     1801 gaagcaattg aaaattgtac tttcatacgt tcacgcgctt tttcatttcc tttttggaaa
     1861 tctgtaagaa caataccagc ttcttttaat gattgcacac tttgatcaac tgcaggctta
     1921 atattgactg ttactatttc atctggttca atgaatcgca aagcttggtc aacttcatca
     1981 gcatcttttt gaactccata aggtaattta actgcaataa acgtacaatc aatgccttct
     2041 tcacgtaatt cgttaacaga catttgtact agttttccaa ctaatgtaga atcttgtcct
     2101 cctgaaatac ctaacactaa agattttata aatgaatgtg attgtacata attttttata
     2161 aattgcttta attccataat ttcttcagca ctatcgatac gctttttcac tttcatttct
     2221 tgtacaataa cgtcttgtaa tttactcatt atcttcttcc atctccttaa cgtgttccgc
     2281 aacttcaaaa atacgtttat gtttattatc ccaacatgcc ttgcttaaat cgactggata
     2341 ttcttgtgga ttaaggaaac gcttattttc atcccaaata gattgtaatc cttgtgctaa
     2401 atattcacgt gattcatctt ctgttggcat ttgatatact aatttaccat tttcataaat
     2461 attatgatgc aaatcaatgg cttcgaaaga ttttataaat ttcattttat aagtatgcac
     2521 tggatggaat aattttaaag gttgttcatc gtatggattt tcattttcca aagtaatata
     2581 atcgccttct gccttacctg ttttcttgtt tataatgcga tatacatttt tcttacctgg
     2641 cgtcgtaacc ttttcagcgt tatttgataa tttaatacga tcactatatg aaccctcttc
     2701 attttcaata gctacaagtt tatatactgc acctaatgct ggttgatcgt atcctgtaat
     2761 cagctttgta ccaacgcccc aagaatctac ttttgcacct tgtgctttca aactcgtaat
     2821 cgtttcttca tccaaatcat tagacgcgat aattttagtt tcagtaaatc ctgcttcatc
     2881 aagcatacgt cttgcctctt tagataaata agcgatatct ccagaatcca atcgaatacc
     2941 tacaaagtta attttgtcac ctaattcttt tgcaactttt attgcatttg gcacgccaga
     3001 ttttaaagta tggaatgtat ctactaggaa cacacaattt ttatgtcttt cagcatattt
     3061 tttgaaggca acatattcat ctccataagt ttggacaaat gcgtgtgcat gtgtaccaga
     3121 cacaggtata ccaaataatt tacctgccct aacattactt gtagaatcaa agcccccgat
     3181 gtaagcagct ctagcgcccc acaatgctgc atcaatttct tgcgcacgac gtgtaccaaa
     3241 ctccattaat ttatcatttg atgcaatttg acgaattcta ctagcctttg ttgtaattag
     3301 tgtatggaaa tttacaatgt ttaataaaat tgtttctatt aattgcgctt gaatcaatgg
     3361 tgcttctacg cgtaacaatg gttcgttacc aaagcataat tcgccttctt gcatcgaacg
     3421 aatgcttcct gtgaatttta aatcttttaa atatgataag aaatcatcct tgtagccaat
     3481 agactttaaa tattccaaat cagattctga aaatccaaaa tgttctataa aatcaatgac
     3541 gcgttttaaa ccattaaaaa cagcatagcc actattaaat ggcatttttc taaaatacaa
     3601 atcaaataca gccatttttt catgaatatt atcattccaa taactttcag ccatatttat
     3661 ttgatataag tcattatgta acattaaact gtcgtcttct aattggtaca cttgtatctc
     3721 tccaatcgac ctaaatattt tcttacattt tatcataatt cattttttta tatacataag
     3781 agccccttaa tttccatact tttaattaaa atcaaccaac aatttaatga catatacata
     3841 atttttaaga gtattttaat aatgtagact ataatataaa gcgaggtgtt gttaatgtta
     3901 tttaaagagg ctcaagcttt catagaaaac atgtataaag agtgtcatta tgaaacgcaa
     3961 attatcaata aacgtttaca tgacattgaa ctagaaataa aagaaactgg gacatataca
     4021 catacagaag aagaacttat ttatggtgct aaaatggctt ggcgtaattc aaatcgttgc
     4081 attggtcgtt tattttggga ttcgttaaat gtcattgatg caagagatgt tactgacgaa
     4141 gcatcgttct tatcatcaat tacttatcat attacacagg ctacaaatga aggtaaatta
     4201 aagccgtata ttactatata tgctccaaag gatggaccta aaattttcaa caatcaatta
     4261 attcgctatg ctggctatga caattgtggt gatcctgctg aaaaagaagt tacacgctta
     4321 gcaaatcact taggttggaa aggaaaaggt actaattttg acgtgttacc actgatttac
     4381 caattaccta atgagtcagt taaattttac gaatatccta cttcattaat taaagaagta
     4441 cctattgaac ataatcatta tccaaaatta agaaaattga acttaaaatg gtatgcagtc
     4501 cctatcattt ccaatatgga cttaaaaatc ggtggcattg tatatccaac tgcacccttt
     4561 aacggttggt atatggtaac tgaaattggc gtacgtaact ttattgatga ttaccgttac
     4621 aatttactag aaaaagttgc agatgcgttt gaatttgata cacttaaaaa taattcattt
     4681 aataaagatc gagcacttgt tgaattgaac tatgctgtgt atcattcctt taaaaaagaa
     4741 ggcgtatcaa ttgtcgatca tttgaccgct gcaaagcaat tcgaactatt cgaacgtaac
     4801 gaagcacaac aaggtcgtca agttaccgga aaatggtctt ggctagcacc gccattatct
     4861 ccaacattga cgtcaaatta tcatcacgga tatgacaata cagtaaaaga tccaaacttt
     4921 ttctataaaa agaaagaatc aaatgctaac cagtgccctt tccatcatta agttagtaga
     4981 ggtgtagcat atgcaattat attatttagg ccctaaaggt acattttcat atttggcttg
     5041 tcgtcagtat ttttctgaaa atgaagcaac ttttcaacca aagtcaaatt tatttgaagt
     5101 catcaaggca gttgcagatg atgacacatc gattggtgtt gtccctattg aaaattctat
     5161 agaaggaaca atcaacattg tggcagatgc acttgcacag caagatgtct ttgcacacgg
     5221 tgaaatacgt ttagatatta actttgcatt atatggtaat ggaacagatt ccatttctga
     5281 tattaaaaaa gtgtattcta ttgcaccagc aatcagtcaa acaacaaatt acatacatca
     5341 acatcaattt gactatgatt atgtcgacag tacaattcaa agtttgacta aaattgaaaa
     5401 tggcgttgca gcaattgcac ctttaggtag tggcgaagct tatggtttta cacctattga
     5461 tacacatatt gaagactatc cacataacgt gacacgtttt ttagttataa aaaatcagca
     5521 gcaatttgat caaaatgcga catcattgat gtttttaatt acaccaatgc atgataaacc
     5581 tgggctgtta gcaagtgtgc ttaatacatt tgctttgttt aatattaatt tatcctggat
     5641 tgagtctcgt cctttaaaaa cacaattagg tatgtatcga tttttcgttc aggctgattc
     5701 tgcaattaca accgatataa agaaagtcat tgctatttta gaaaccttag atttcaaagt
     5761 agaaatgatt ggtgctttta attaaacttt attttctagg ttctacaatt taaataaaat
     5821 aaagagtcaa ccaaggatta aaataggaat ccatccttgg ttgactttct tttgtttgta
     5881 ttgtttttac acgatgctgc ggtatacgtt tcattaccac tcgttcaagc aattgctaat
     5941 gtaacctttt atttttcgtt ctaatctgca attactattt caatggcagt ggttgattta
     6001 tatcaatacc taaaactata ggcattacat aatacacgac taaaataata attattgcac
     6061 tgattaagtt tacccagaat cctactgatg ccatttgttt gatagatatt ttacctgaac
     6121 caaagataat tgcattcggt ggtgtcccta ctggtaacat gtatgcacag ttagccgcca
     6181 tagctgcagg tgccataagt agtaatggat gcactccaac agcaacagac aacgttgcta
     6241 aaatcggtaa aatcatcgtt gcagttgcag tattagatgt cacttcagtt aaaaataaga
     6301 caaagattgt tatgacaatt acaataagaa tcggactaac accatttaat gatttcaact
     6361 gttcgcctaa ccattttgct aaaccacttt cagaaatacc tttcgctaat gctaaaccgc
     6421 caccaaataa aattaataca ccccaaggga gctcttttgc aacttcccag tcaatgatac
     6481 ggcgatgttt ttcagtattt ttagctggaa taataaataa taatattgat ataaaaatag
     6541 caatcgtacc atctgcaaca gatgacgtaa cttcccattt tttcagaaga aactctcttg
     6601 taatccataa taagctggca agtacaaaga tagtttgtac taccttttct tcatacttca
     6661 ttttgccaag ctcgtctaac ttttgtttaa ttaacgtctg accaccaggt aaatatttca
     6721 aatcatgtct aaacgcaaca tatcttaaat agagccaagt aatacctaac aaaacaatga
     6781 ccgttggaat ccctacaatc atccatttag caaaactaat ttcatgtcca aaatgttgca
     6841 tgtattgtcc ttttaaaata attaatggcg gggttccgat taatgtaccc aagccaccaa
     6901 tcgtacctgc atagccaatt gctaaaacta gagatttttc aaacttttga atacttgttt
     6961 gattcgtatt ggcttcttgt aaatcatgtg cttccttaat aattgctaaa ccaatcggaa
     7021 tcataatcat tacagctgca gtgttcgata caaacataga taagaatcct gttgccacca
     7081 tgaatccaag taatattttt gaagtgcttg caccaattaa attaataata gtcaaagcta
     7141 ctctcgtatg taaattccat ctttccattg caattgccaa aataaatcca cctaaaaaca
     7201 aaaagataat atcattgcca tattcggatg atacttgttc tggtgtaagt atgtgaccta
     7261 atggtaataa cacaattggt aataagctcg ttgctgcaat aggaattgct tcagtaatcc
     7321 accaagtcgc aatccataaa gtaatcgcta agacatatac gcctttccaa ggtaagtctt
     7381 gtggatgaaa gaacaataat gttaatagga aaagtaatgg acctaatatt agtccaataa
     7441 gttgtcccgc actaaacggt ttctttttct ttgattttga aaagaatgtt aaataccttt
     7501 tcgatatcat tgattgatgt tgattgaaat aagccataaa ttttccccct catgatgatt
     7561 atgacaagct acttcaactc actatattag agtatagaaa gttgtctatc ttgtattatc
     7621 attaccgtca aaatacccct tttatcagat aattgggttt attatagcat atttataccg
     7681 ttttatgaat gcgctttcaa aaattatcta ctattcttaa tcctaatgtc atgcgaaact
     7741 ttttactttt ttatttcatc tatattgatt gtagtaactt gaatacccgc ttcaacagga
     7801 cttaaaaaca ctgtatcaat atcttctaaa tgaattgatt gagtcgaatt attgaatgta
     7861 ttcccaacga ttaatacatc tttatgttta acattattat aattacgtac atgtatcgca
     7921 tctttagaca ttgatccttt aaattcattt ccaattatat tcatatttat gcctgcttgg
     7981 gaacctaagt cttttcctgt catcacatct gctgcatttt taccatctct aactcctaaa
     8041 tatctaatac caccctcaca gttaataaac ttattattaa taatgaaagc atctttatac
     8101 ttcaagggag ttaatgcata accttgtata tcttcaaata tattatttct aatatgaata
     8161 ttctcatagt aacgattatg tctacttgca tgtgagccaa tagcacgatt ccaacttccc
     8221 atttcgggca attctgaagg tccaaaataa caaccttcga taacgacatt tttcgttatc
     8281 gtaccatctg tcgttccgaa ttttggaaaa gcaccaggta cttgaatgtc taattgtatt
     8341 gcttcagaat aaaagcgttc gccactatag tctatgaatc cttcaaatga acagctttta
     8401 atatagagtc cgttaatccc acaagcatca attgcatgac cactcactac attcttaatg
     8461 gtcacaccta ttaattgaat atcttcagca tgcccaatgc acatagctgt attgttataa
     8521 ggatacgata caccattcat atcaaacttg ccgcctttaa tatgaatatg actgtgtcca
     8581 ttataaccac gataaaaacc aaagcgatga ccatttttta ataaaggacc agaatgacat
     8641 cggcgtaaaa tagtttcatt atctaacaaa agtgttgtat tgccatatat cgttaatggt
     8701 ttgcaaatat catacgtccc tttcggtata taaactgtcg ttggtttaca tcttccacga
     8761 tttaacgcac gttgtattgc tcgagtatct ttagttttgt ttatgcctgt taacccataa
     8821 tctttcatat tgatatacat aaatcgtcat cctttgtcta ttttctaatt actcatatat
     8881 taaccaatta ttcaaattat aatcgtgtta ggttgattta gcagatagtc ataaatctcg
     8941 tgatgccatc tattagcatt tttatattca aaacatcaac acttgatatt agttctaagt
     9001 ttgtcacatt tagtattcta ttacagctat tttaaaacta tcgcaaaaac ttcttatatt
     9061 atttgttgat tgcgtgattt gctcttccaa aaaagttgat taatctatat acttaatcta
     9121 tatttatata ttaaccatta gggttaaaaa ttactctagc atttatgaat agatgggagt
     9181 ttattttatt atataggaga gatgttgaat gacacatcgc gcactattag ttgttgacta
     9241 ttcatatgac tttatcgcag atgacggctt actaacatgc ggtaaacctg gacaaaatat
     9301 tgaagatttt attgtttctc gtatcaatga ctttaattat tatcaagacc atatattctt
     9361 tttaatggat ttacattatt tacatgacat tcatcatcct gaaagtaaat tattcccacc
     9421 acacaatatc gtagatacaa gtggtagaga attatacggt aaagtaggta aattatacga
     9481 aacaattaaa gcgcaaccta atgtacattt cattgataaa acgcgctatg attcgttctt
     9541 tggtaccccg cttgatagtt tattgagaga aagaagtatt aatcaagtcg aaatcgttgg
     9601 tgtatgtacc gatatttgcg tgttacatac agcaatttct gcatacaact taggttataa
     9661 aatttcagta cctgctgagg gagtggcttc atttaatcaa aaagggcatg aatgggcact
     9721 tgcacatttc aaaaactcat taggtgcaga ggtagaacaa cacgtttaaa tcgtgctaaa
     9781 ataattataa agaatacaat ttacaaggga gatatttgac aatggctaaa acatatattt
     9841 tcggacataa gaatccagac actgatgcaa tttcatctgc gattattatg gcagaatttg
     9901 aacaacttag aggtaattca ggagccaaag cataccgttt aggtgatgtg agtgcagaaa
     9961 ctcaattcgc gttagataca tttaatgtac ctgctccgga attattaacg gatgatttag
    10021 atggtcaaga tgttatctta gttgatcata acgaattcca acaaagttct gatacgattg
    10081 cctctgctac aattaagcat gtaattgatc atcacagaat tgcaaatttc gaaactgctg
    10141 gccctttatg ttatcgtgct gaaccagttg gttgtacagc tacaatttta tacaaaatgt
    10201 ttagagaacg tggctttgaa attaaacctg aaattgccgg tttaatgtta tcagcaatta
    10261 tctcagatag cttacttttc aaatcaccaa catgtacaca acaagacgtt aaagcagctg
    10321 aagaattaaa agatattgct aaagttgata ttcaaaagta cggcttagat atgttaaaag
    10381 caggtgcttc aacaactgat aaatcagttg aattcttatt aaacatggat gctaaatcat
    10441 ttactatggg tgactatgtg actcgtattg cacaagttaa tgctgttaac cttgacgaag
    10501 tgttaaatcg taaagaagat ttagaaaaag aaatgttagc tgtaagtgca caagaaaaat
    10561 atgacttatt tgtacttgtt gttactgaca tcattaatag tgattctaaa attttagttg
    10621 taggtgctga aaaagataaa gtcggcgaag cattcaatgt tcaattagaa gatgacatgg
    10681 ccttcttatc tggtgttgtt tctcgaaaaa aacaaatcgt acctcaaatc actgaagcat
    10741 taacaaaata atactatatt actgtctaat catagacatg ttgtatttaa ctaacagttc
    10801 attaaagtag aatttatttc actttcaatg aactgttttt tatttacgtt tgactaattt
    10861 acaacctttt caatagtagt tttcattcct ttagctatcc taacccacag attagtgatt
    10921 tctatacaat tcctcttttg tctttacatt ttcttaaaat atttgcgatg ttgagtataa
    10981 atttttgttt tcttcctacc tttttcgtta tgattaaagt tataaatatt attatgtaca
    11041 cgattcatcg ctctattttc aactttcaac atatataatt cgaaagacca tttaaaatta
    11101 acggccacaa caatcaaatc aattaatcac tttttccaaa ataatcatat aaggaggttc
    11161 ttttcattat gaatatcatt gagcaaaaat tttatgacag taaagctttt ttcaatacac
    11221 aacaaactaa agatattagt tttagaaaag atcaattaaa gaagttaagc aaagctatta
    11281 aatcatacga gagcgatatt ttagaagcac tatatacaga tttaggaaaa aataaagtcg
    11341 aagcttatgc tactgaaatt ggcataactt tgaaaagtat caaaaatgcc cgtaaggaac
    11401 ttaaaaactg gactaaaaca aaaaatgtag acacaccttt atatttattt ccaacaaaaa
    11461 gctatatcaa aaaagaacct tatggaacag ttttgatcat tgcaccattt aactatcctt
    11521 ttcaactagt attcgaacct ttaatcggtg ctattgcagc aggtaataca gcaattatta
    11581 aaccatctga gttgacacca aatgttgcac gagtgattaa acgattaatc aatgaaacat
    11641 ttgatgcaaa ttacattgaa gttattgagg gaggaattga agaaacgcaa acgttaattc
    11701 acttaccttt tgactatgtc ttctttacag gaagtgaaaa tgtaggcaaa atcgtttatc
    11761 aagctgccag cgaaaattta gttcctgtga cattagaaat gggtggtaaa tctccagtca
    11821 tcgttgatga aacagcgaat attaaagttg ctagtgagcg catttgtttt gggaaattca
    11881 ctaatgccgg ccaaacatgt gttgcaccag attacatttt agtacacgaa tctgtaaaag
    11941 atgatttaat cacagcccta tcaaaaacgt tgcgtgaatt ttatggtcaa aatatacaac
    12001 aaagtccaga ttatggccgc attgtaaacc ttaaacatta tcatcgtctg acttcattac
    12061 ttaacagtgc acaaatgaat attgtatttg gtggtcatag tgatgaggat gaacgttata
    12121 tagaaccaac attgttagat cacgttacaa gtgattcagc aattatgcaa gaagaaattt
    12181 ttggtcctat cttaccgatt ttaacgtatc agtcattgga tgaagcaata gcctttattc
    12241 accaaagacc aaaacctttg agtttatatt tatttagcga agatgaaaat gctacacaac
    12301 gtgtaataaa cgagttatca tttggcggcg gcgctattaa tgatacattg atgcacctag
    12361 cgaatcctaa attaccattt ggtggtgttg gcgcctcagg tatgggacgc tatcatggta
    12421 aatattcatt cgacactttt acacatgaaa aaagctacat tttcaaatct acacgattag
    12481 aatcaggtgt ccatttacca ccatataaag gtaaatttaa atacatcaaa gctttcttta
    12541 aaaattaatt tttgagacaa aaataaacca tgcttccata atttgatgtt cttgaaaaca
    12601 ataaatttca aggtatacac aaattttttg taaacatggt tttcttttta tcatgattaa
    12661 ttaaagatta aaatatgaca catacacctt cagatgctac acgagtatta tcacatagag
    12721 taattttacc tgtttcttta cttctttcaa aaacagttac aactgaatct ccttgctcat
    12781 gagcacaaac taaaaggtca tctgatgagg caatattaaa atctcttggg aattgaccac
    12841 cactttcagt aattgttact agttctaagt gttgaccatt atcaagaact ttaaaaattg
    12901 caatgctatc atgccctcta ttagatacat ataagaattg ttgatcatga gacaaacgca
    12961 ctgctgcaag tttagtatct ccatcaaagt tttcaggaat tgttaaatga cgctcgagct
    13021 cttcaaattt accgtcatta tattctgcaa cactcacagt atttgataat tcgtgtacga
    13081 cataagcaaa ttttccatta tcatgaaatt caatatgtct tgtcccatca ctatctttaa
    13141 ataaagattc tttataaaat tcaaacccgt tgtcatcaaa tttataagta acgatacgat
    13201 cagcacctaa atctgttact gcaacgtact taccatctgg agtttgatta atataatgtg
    13261 cgtgtggatg atcttgtctt tcatgtgtac ctgttggaaa atcatgtgca agttcttgaa
    13321 ttagacgtat aatttcacct gtgtgcgtat ttaattcata catacgtatg atgccagcac
    13381 catataccgc ttcaaataaa tatcgtttat cttctgaaat cgatacataa caacctgtac
    13441 cagcttttga agacaaacat ttatttaata aatgcaattc accattgtca tcaattttca
    13501 aactcgcaac accgcattgt tctccttctt tgttgattcc atataaaact tcgttattac
    13561 gcaccaaata tgtagacgct tctaattcaa atcctatttc taataaatca atacgtgact
    13621 gattttcgtt taattcaaaa cgataaatcc ctttaccatt ctttttagtg taagaaccaa
    13681 tatatccatt tgtcattact tttacccctc catgtcagtc ttattttcat tttattaaat
    13741 tttagctaga attaacaatc atttatcatt tccttaacgc taaaactgtt taactacttt
    13801 aaaagaacta tttgctgtga tatgtcatta ttcgatttta attaatccct cttttttact
    13861 tacacgagca ttaattatta acgcgtttat gcttccacaa aatttttgtt gcaaaacaga
    13921 acacatgttc gtataatgta attatcagat tggggtgaat taaggatgac aagttatgaa
    13981 gaagaaactg attatcgtaa cgttccaaga gaattactag ataacaatat acctatggga
    14041 agaggcatga ttaaatgggc tccttttgca acattacccg aacaattcga aacgattcaa
    14101 caatacatta tcgaccaaaa caaaattaca cgtcctgtat tatcagatga tcaattggct
    14161 gagctaaaca ttcggctaca cgaggcgcta caatatgccc aacctgtaga agttaaattt
    14221 tataataatg gctttgtaga ttcagtacgc ttaaccattt atcgtattga tgctattaat
    14281 tatgaaattg atggttatgt ttacaatcag caacaacgtc aaaaaatttc tatctttgat
    14341 atcttagata ttgtcttgtt accttaatgc tttgaacata attaaacaga ttgagtatat
    14401 gcatataaga tgatttttaa catcattttt ggatgatagg atgttgcgcc acgatgatgt
    14461 ctgaattcat cgaattcgct atcaggtatt gtttcaacaa tttcattaac atatcgtgaa
    14521 atatcatttt gagggattct aacagaagtt tctattggta gtgtagttgg gtcatgttat
    14581 aatttttata cataaggcac ctcgttaatt tagtttagtg gtatttatta aattataaga
    14641 agggacccaa cacagaaaat tcattttatt gaattttaca tttatgtgca agttgggcaa
    14701 agtgtcttat tttttaaaag tatttcaaag taaaattaca tgttaatacg tagtattaat
    14761 ggcgagactc ctgagggagc agtgccagtc gaagaccaag gctgagacgg caccctagga
    14821 aagcgaagcc attcaatacg aagtattgta taaatagaga acagcagtaa gatatt
//
LOCUS       DANBWH010000050        14832 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.052, whole genome shotgun sequence.
ACCESSION   DANBWH010000050 DANBWH010000000
VERSION     DANBWH010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14832
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.052"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..941
                     /gene="hlgC"
                     /locus_tag="PPY92_001051"
     CDS             <1..941
                     /gene="hlgC"
                     /locus_tag="PPY92_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgCB subunit C"
                     /protein_id="HDJ2625068.1"
                     /translation="NKILATTLSVSLLAPLANPLLENAKAANDTEDIGKGSDIEIIKR
                     TEDKTSNKWGVTQNIQFDFVKDKKYNKDALILKMQGFISSRTTYYNYKKTNHVKAMRW
                     PFQYNIGLKTNDKYVSLINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGGNGSFNYS
                     KSISYTQQNYVSEVEQQNSKSVLWGVKANSFATESGQKSAFDSDLFVGYKPHSKDPRD
                     YFVPDSELPPLVQSGFNPSFIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTHYGN
                     SYLDGHRVHNAFVNRNYTVKYEVNWKTHEIKVKGQN"
     gene            943..1920
                     /gene="hlgB"
                     /locus_tag="PPY92_001052"
     CDS             943..1920
                     /gene="hlgB"
                     /locus_tag="PPY92_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB/HlgCB subunit
                     B"
                     /protein_id="HDJ2625069.1"
                     /translation="MKMNKLVKSSVATSMALLLLSGTANAEGKITPVSVKKVDDKVTL
                     YKTTATADSDKFKISQILTFNFIKDKSYDKDTLVLKATGNINSGFVKPNPNDYDFSKL
                     YWGAKYNVSISSQSNDSVNVVDYAPKNQNEEFQVQNTLGYTFGGDISISNGLSGGLNG
                     NTAFSETINYKQESYRTTLSRNTNYKNVGWGVEAHKIMNNGWGPYGRDSFHPTYGNEL
                     FLAGRQSSAYAGQNFIAQHQMPLLSRSNFNPEFLSVLSHRQDGAKKSKITVTYQREMD
                     LYQIRWNGFYWAGANYKNFKTRTFKSTYEIDWENHKVKLLDTKETENNK"
     gene            complement(1972..2439)
                     /locus_tag="PPY92_001053"
     CDS             complement(1972..2439)
                     /locus_tag="PPY92_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDJ2625070.1"
                     /translation="MTSKDITQISVIAAILTILAVLKIPSIIPGLDFQLSAPAALLIL
                     AFFGIKKYFLGGLLSSVLLLIFGVFNPINVIISIIFRVIAIAVVYLLKINVLSLVLSS
                     VLGSLVSRLLLSIILNLPVWVVLLNAIPGVIFTLIVAIPLYLTLRKRMAVLLR"
     gene            complement(2450..3142)
                     /locus_tag="PPY92_001054"
     CDS             complement(2450..3142)
                     /locus_tag="PPY92_001054"
                     /EC_number="6.2.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxyhexanoate--CoA ligase"
                     /protein_id="HDJ2625071.1"
                     /translation="MYSIKMRSSNQDVHISGAETICEFDKIEQTVQRFYNKGFFHENG
                     QPDFLNIKIQKIMEPIQQIKALQIIEDDKANLQHLTQECGVTEQALNQGMTYIKNETV
                     YTGAIILSAISGKRLDSFGQRGIRATHFSFEDINNKGDLNERVTDALAIASCINAHPY
                     VKGELCVSDDLTYTTGYFAAAKIGYHRLFDIKPVNTRYGGRIIFVDDCIDLNHYISFL
                     ESTPKQVVYETV"
     gene            complement(3153..4268)
                     /locus_tag="PPY92_001055"
     CDS             complement(3153..4268)
                     /locus_tag="PPY92_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HDJ2625072.1"
                     /translation="MMFQEQIDLLQQKGLYRSLKSVGYIDRQYIEVENKRCTNYTSND
                     YLGLGQIAFDKDDFERFMRKYSYHLSSSRLISGSSTAYEEIETMLAGWLGYSACTILN
                     SGYDANLALFNIFKNTNCVVFSDQENHASIIDGIKLSGLEKVIYKHLDIADLEKRLEK
                     YPNQNIPKIIISDSVFSTNGDVVDIGQLVSLKHKYNATLILDVSHSFGIENYSNYQGV
                     DILTSSLSKACGAYGGVILSSNDVKDMLINHGRPLIYSSSLPIYNLYFIKRNIEKLIN
                     ADDRRTKLNSLSKYFNQKLKALNVNYNSSNSPIKFIEFDDIEAAENIHQTLLKHHVFT
                     SYLRYPTVTKPMLRISLSYFHTEQDIDRLFEILHQED"
     gene            complement(4246..5253)
                     /gene="bioB"
                     /locus_tag="PPY92_001056"
     CDS             complement(4246..5253)
                     /gene="bioB"
                     /locus_tag="PPY92_001056"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDJ2625073.1"
                     /translation="MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYF
                     GKKVKLNMILNAKSGICPENCGYCGQSRDIKQKQRYALIPEEQIIDGAKVAHDNHIGT
                     YCIVMSGRGPSDKEVDHISNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYN
                     HNINTSENYHDNVVTTHSYKDRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFAL
                     KEMDADSIPINFLHPIKGTKFGSMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNL
                     RSLQPLALKAANSIFVGDYLITGGQPNQLDYDMINDLGFEIDYDTCENKENKNDVSRA
                     N"
     gene            complement(5255..6613)
                     /gene="bioA"
                     /locus_tag="PPY92_001057"
     CDS             complement(5255..6613)
                     /gene="bioA"
                     /locus_tag="PPY92_001057"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HDJ2625074.1"
                     /translation="MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDT
                     NGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIAHSTLLGSSNIPSIELAEKLIEI
                     TPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAV
                     SVGGIKTFHKIFKDLIFENIQVESPSFYRSNYDTENEIMTAILTNIEQILIERNDEIA
                     GFILEPLIQGATGLFVHPKGFLKEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHED
                     VQPDIMCLGKAITGGYLPLAATLTSQKIYNTFLSDSHGVNTFFHGHTYTGNQIVCTVA
                     LENIRLYEKRKLLSHIETTSSTLEKQLHALKRHRNVGDVRGRGLMFGVELVTDKDSKT
                     PLEIEKVERIVRKCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDR
                     KC"
     gene            complement(6591..7277)
                     /gene="bioD"
                     /locus_tag="PPY92_001058"
     CDS             complement(6591..7277)
                     /gene="bioD"
                     /locus_tag="PPY92_001058"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HDJ2625075.1"
                     /translation="MRIFITSTNTDVGKTYVTKHLYHALKTRGHRVCVFKPFQTEERQ
                     DGTFPDLEVFKNECDLSYDITSLYTFKQPVSPHLAFKMTDQIFLNKQRVLDKVKVLDK
                     EFDFILIEGAGGIAVPIYEGTDDFYMTKDLINDCADCVISVLPSKLGAISDAVVHQDY
                     VNKNVSASNFLIMNRYTDSYIEKDNQITIGKLTNKTVYTFEEHATYENFSEAFLKQLI
                     GVKNELHTTT"
     gene            complement(7332..7499)
                     /locus_tag="PPY92_001059"
                     /pseudo
     CDS             complement(7332..7499)
                     /locus_tag="PPY92_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501179.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7730..9463)
                     /locus_tag="PPY92_001060"
     CDS             complement(7730..9463)
                     /locus_tag="PPY92_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDJ2625076.1"
                     /translation="MFQITFKILNWIRPYKARMILGFSMSFLNAIFIALPIFLAAKIF
                     NNVLSHKPIYMKDILNVVIIMVLLVIGRFITAYFKSKSHESIAYEMSAKERLDIGDKL
                     KNVRLGYFNSHHSNELTTIVTTDLTFLENFAMKMVDIVVNGYILITVLILSLLVVSWQ
                     VSLLACIGVLLSFFAIQLLERKSRQNAPAYHNVQNQLVEKVLEVIRGIQVIKSFAKEN
                     TSLKSFNQAVNESKRINTKIEMQYIPFNLLHLLSLKVVSIMIVLVACLLYMNHSIDLP
                     TLIMISIFSFVIFDSVENINSAAHVLEMIDMTIDDIEKIKNAPELDENGKNLTIKNEN
                     IAFQNVNFSYDDKQVIKNVNFEIPTQTSTAIIGPSGSGKSTLCHLLLRFYDIDDGNIR
                     IDGVDIKDMTLSTLMSKISAVFQKVYLFNDTIENNILFGNPGATKEEIIRAAKQACCH
                     DFIMSLPEGYQTMLNEKGSNLSGGEKQRISIARAILKDAPIIILDEATASIDPENEQL
                     IQTAINELSKGKTVITIAHKLETIKNADQIIVLNEGEIIQKGSHDELIRKPGMYQDFI
                     RIKSKSAGWKL"
     gene            complement(9488..11251)
                     /locus_tag="PPY92_001061"
     CDS             complement(9488..11251)
                     /locus_tag="PPY92_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDJ2625077.1"
                     /translation="MNNQTNWIKILSGFASDSKWKIMLSILLSIISVFSGLVPYWAVF
                     KIILMMINNAYTINSIMVYIFIALITYISQVCCFGASTMLSHITAYEILSEIRKKLAQ
                     KLMRLPLGVVESKKIGELKNIFVDKVETIELPLAHMIPEVIGNLLLSAAIFLYIMLID
                     WRMASALLVTIPISIFAFKKVMSGFNETYAEQMKSNNYMNSAIVEFIEGIEVIKTFNQ
                     SQSSYKKYKDAVDNYKIHTLNWFKNTWGYMNLGASVLPSTFLGILPVGMYLISINQLN
                     YAEFFLCIVLSLGVVAPIKNFTNYVNHLKSIQYALTEVNQILSLEELVLSTKFKKPQH
                     YEIAFNNVGFSYNKDKDDLVFKHLSFTVPENNFTAIVGASGSGKSTIAKLISRYWDVT
                     SGEITIGGINIKDIESKQLNDLVGFVGQDNFLLNLTFKENIKLGNPEATDEAVEKAAK
                     LAQCHEFIEKLPDGYDTNVGTVGDKLSGGEKQRVTIARMILKDAPIIVLDEATAYVDP
                     DNEQKIQEALNVLTQDKTLIVIAHRLSTIQHADQIIVLGKQQILEKGSHHLLLKLNGN
                     YKKMWDTHMHTKDWGINTGHN"
     gene            complement(11954..12061)
                     /locus_tag="PPY92_001062"
     CDS             complement(11954..12061)
                     /locus_tag="PPY92_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDJ2625078.1"
                     /translation="MFNLLINIMTSALSGCLVAFFAHWLRTRNNKKGDK"
     gene            complement(12195..12581)
                     /gene="gtxA"
                     /locus_tag="PPY92_001063"
     CDS             complement(12195..12581)
                     /gene="gtxA"
                     /locus_tag="PPY92_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase GtxA"
                     /protein_id="HDJ2625079.1"
                     /translation="MKLTQTHAEILKFIIVGGINTLNYYVVYLLLLKLLHIEYMISHI
                     TGFLVAFVISYYLNCYFVYRVKPTWRKFISFPITQIVNVSLQTVLLYVFVSWLNLPAE
                     IAPFAGLVITIPITFVLSKWILKDSN"
     gene            12849..13991
                     /locus_tag="PPY92_001064"
     CDS             12849..13991
                     /locus_tag="PPY92_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDJ2625080.1"
                     /translation="MQLQKIVIAPDSFKESMTAQQVGNIIKQAFTNVYGNTLHYDIIP
                     MADGGEGTTDALMHATGATKYTVIVNDPLMRPIEACYARADEQQIAIIEMAAASGLDL
                     LEKEERNPLYTSSYGTGELIKDALNHGAKTIILGIGGSATNDGGTGMLSALGVKFTDV
                     NGDLLQMNGANLAHIAQIDITNLDSRLKEVTFKVACDVSNPLLGENGATYIYGPQKGA
                     DAKMIPKLDFAMSHYHDKIKMCTGKSVNQIPGSGAAGGMGAALLAFCETTLTKGIDVV
                     FDITDFHQRIKDADLVITGEGRMDYQTIFGKTPVGVALAAKQYHIPVIAICGSLGENY
                     QHVYDFGIDSAYSIISSPSTLEDVLQNSEQNLLNTATDIARILKLQ"
     gene            14051..14710
                     /locus_tag="PPY92_001065"
     CDS             14051..14710
                     /locus_tag="PPY92_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625081.1"
                     /translation="MKRTDKYRDSYQYDNQNQNHRRQSEDASYRQQYAKGDPEEHPER
                     YYNGRDYRREQILEEENEKSRRSKKWLYIIIAILLIIVAIFVTRALLNNDSDKVSNDP
                     KVSQNYKKQVENQDGQINQQVDNAKENIKNNQKTDDIIKNLQNQIDNLKQQEQNKADS
                     KLTQFYQDQINKLTEANNALKNNASQGKIESMLNDINTKFDSIKSKLESLFKDDNGGA
                     N"
BASE COUNT         4989 a         2672 c         2116 g         5055 t
ORIGIN      
        1 aaaataaaat attagctaca actttatctg tgagcttact tgcccctctt gccaatccgt
       61 tattagaaaa tgctaaagct gccaacgata ctgaagacat cggtaaagga agcgatatag
      121 aaattatcaa aaggacagaa gataaaacaa gtaataaatg gggcgtgact caaaatattc
      181 aatttgattt tgtgaaggat aaaaaatata acaaagatgc tctgatatta aagatgcaag
      241 gattcattag ctctagaaca acatattaca actataaaaa aactaatcat gttaaagcta
      301 tgcgatggcc attccaatat aatattggtt taaaaacaaa tgataaatat gtttctttaa
      361 ttaattattt acctaaaaat aaaattgaat ctacaaacgt gagtcagaca ttaggataca
      421 atatcggtgg taatttccaa tcagccccat cactcggtgg taatggatca tttaactatt
      481 ctaaatcgat tagctataca caacaaaatt atgtaagtga agtagaacaa caaaactcaa
      541 aaagtgtttt atggggcgtc aaagcgaatt cattcgccac tgaatcaggt caaaaatcag
      601 catttgatag cgatttattt gtaggctaca aacctcatag taaagatcct agagattatt
      661 tcgttccaga cagtgagtta cctcctcttg tacaaagtgg atttaaccct tcatttatcg
      721 ccacagtatc tcatgaaaaa ggttcaagcg atacaagcga atttgaaatt acttacggaa
      781 gaaacatgga tgtcactcat gccattaaaa gatcaacgca ttatggcaac agttatttag
      841 acggacatag agtccataat gcattcgtaa atagaaacta tactgttaaa tacgaggtca
      901 attggaagac tcatgaaatc aaggtgaaag gacagaattg atatgaaaat gaataaatta
      961 gtcaaatcat ccgttgctac atctatggca ttattattac tttctggtac tgctaatgct
     1021 gaaggtaaaa taacaccagt cagcgtaaaa aaagtcgatg acaaagttac tttatacaaa
     1081 acaacagcca cagcagattc tgataagttt aaaatttcac agattttaac atttaatttc
     1141 atcaaagata aaagttatga taaagatact ttagtactta aagctactgg gaatattaac
     1201 tcaggctttg tgaaacctaa tcctaatgac tatgactttt caaaattata ttggggagct
     1261 aaatacaatg tatctataag ctcacaatct aatgattcag taaacgtcgt tgattatgca
     1321 ccaaaaaatc aaaatgaaga gtttcaagtt caaaatactt taggctatac atttggtggt
     1381 gacattagta tctctaatgg tttatctggt ggacttaatg gaaatacagc tttttctgaa
     1441 acaattaatt ataaacaaga aagttacaga acaacattaa gtcgcaacac aaattataaa
     1501 aatgttggct ggggagttga agcacataaa attatgaata atggttgggg accttatgga
     1561 agagatagct tccacccaac atatggtaat gaactcttct tagctggcag acaaagcagt
     1621 gcatacgctg gccaaaactt catagcgcaa caccaaatgc cattattatc tagaagtaac
     1681 ttcaatccag aatttttaag cgtactatca cacagacaag atggcgctaa aaaatctaaa
     1741 attacagtaa cttatcaacg tgaaatggat ttataccaaa ttcgttggaa tggcttctac
     1801 tgggcaggcg caaattataa aaactttaaa actagaacat ttaaatcaac atatgaaatt
     1861 gattgggaaa atcacaaagt gaaattgtta gatacaaaag aaactgaaaa caataaatag
     1921 ctagtaaaac acggtcgcca acagtaattg tgacgaccgt gttttgattt attatcttag
     1981 taagactgcc attctttttc tcaatgtgag atataaagga atagctacaa ttaaagtgaa
     2041 tattacgcct ggaatcgcgt ttaacaacac tacccacaca ggtaaattta aaataataga
     2101 taatagtagc ctagatacca aactgcctaa tacacttgat aaaactaatg atagtacatt
     2161 tattttcaat aaataaacaa ctgcaatagc tataactcta aatataatag aaataatcac
     2221 attaattgga ttaaatacgc caaatatcag taataatacg cttgataaaa gcccacctaa
     2281 aaagtacttt ttaattccaa agaaagctaa tatcaataat gctgccggtg cagataattg
     2341 aaaatctaat cctggtataa tggacggtat tttcaaaact gccaaaatgg ttaaaatcgc
     2401 agcaatgaca ctaatttgag taatatcttt tgatgtcata ctaaaacccc tataccgttt
     2461 cataaacaac ttgcttcggt gtgctttcta aaaatgatat gtaatgattt aaatcaatac
     2521 aatcgtccac aaatattatt ctgcctccat atctcgtatt aactggttta atatcaaata
     2581 atcgatggta accaatttta gcagcggcaa aataacctgt cgtatacgtt aagtcatcgg
     2641 acacgcaaag ttctcctttg acatacggat gcgcattgat acaactagca attgctaagg
     2701 catcagtcac tctttcatta agatcacctt tattatttat atcttcaaac gaaaaatgtg
     2761 ttgccctaat ccccctttgt ccaaatgaat ctaaacgttt accagatata gcagatagaa
     2821 taatagctcc tgtataaacc gtttcatttt taatatatgt catcccttga tttagcgctt
     2881 gttcagtgac accacattct tgtgttaaat gttggagatt tgctttatca tcctcaataa
     2941 tttgtaatgc ttttatttgt tgaatcggtt ccataatttt ttgtattttt atattgagaa
     3001 aatccggttg cccattttca tgaaaaaaac ctttattata aaacctttgt accgtctgtt
     3061 ctattttgtc aaactcacaa atcgtttcag caccactaat atgaacatct tgattgctag
     3121 aacgcatttt tatactatac atgacgatca cctcaatctt cttgatgcaa aatttcaaac
     3181 aacctatcta tatcttgttc agtatgaaaa tacgacaatg atattcttaa cattggctta
     3241 gtcacagttg gataccttaa ataacttgta aacacatgat gctttaataa tgtttgatga
     3301 atgttctcag ccgcttctat gtcatcaaac tcaataaact taatcggcga gtttgaacta
     3361 ttataattaa cattgagtgc ttttaacttt tggttaaaat atttactcaa actatttaat
     3421 ttagtgcgtc tatcatcagc atttattaac ttttcaatgt ttctttttat aaaatacaaa
     3481 ttataaattg gcaaactact tgagtagatg agtggtctac cgtgattaat taacatatcc
     3541 ttcacatcat ttgaacttaa aatcacaccc ccgtatgcac cacatgcttt agataaacta
     3601 gaagtgagta tatctacacc ttgataattc gagtaattct ctattccaaa actatgtgaa
     3661 acatcgagta tcagtgttgc gttatattta tgctttaatg agactaattg accaatatcc
     3721 acaacgtcgc cattcgttga aaatacacta tcagatatga ttatttttgg tatattttga
     3781 ttagggtatt tctctaacct tttttctaaa tcagcaatat ctaaatgctt atatatcact
     3841 ttttctaaac cacttaactt aataccgtca ataatactcg catgattttc ttgatctgaa
     3901 aacacgacac aatttgtatt tttgaaaata ttaaacaacg ccaaattagc atcataacca
     3961 ctatttaaga tagtacatgc actatatccg agccaacctg ctaacattgt ttcaatttct
     4021 tcataagctg tcgaacttcc actaattaat cttgaacttg ataagtgata actatacttc
     4081 cgcataaatc tttcgaaatc atccttatca aacgctattt gacctaatcc taaataatca
     4141 ttagatgtat agttcgtaca tctcttattt tctacttcaa tatactgtct atctatatac
     4201 cctaccgatt taagcgaccg atataaccct ttctgttgta ataaatcaat ttgctcttga
     4261 aacatcattc ttgttttcct tattttcaca agtgtcataa tcaatttcaa agcccaaatc
     4321 attaatcata tcgtagtcta attggttcgg ttgcccacca gtaattagat aatcaccgac
     4381 aaatattgaa ttcgccgctt ttaatgctaa tggctgtaac gaacgtaagt tgacctctct
     4441 tcctccagca atacgaattt ctttcgtagg attgattaat cggaataatg ctacgattct
     4501 taaacatttc attggtgtta aatcatccat gcttccaaac tttgtgcctt tgattggatg
     4561 caaaaaatta atcggaatac tgtcggcatc catttctttt aaagcaaatg ccatatcaac
     4621 aatatcttga ttagattctc ccataccaca aatcacgcca gaacatggtg atatattatt
     4681 cgctttcatt agttctatcg tatctgttct atctttataa ctatgcgttg tcacgacgtt
     4741 atcatggtaa ttttcacttg tattaatatt gtggttatat ctgtctacac cagctgactt
     4801 aagtttctta gcttgttcgt cattcgttaa tcctaaacat gcacagattt ttagttgcgg
     4861 gtgttgagat ttaatcgttc ttacagtatt actaatatga tcaacttctt tatcgctcgg
     4921 tcctctacca ctcataacaa tacaatatgt tccaatatga ttatcatgtg ccacctttgc
     4981 tccatcgata atttgttcct ctggaattaa agcatatcgc tgtttttgtt taatatctcg
     5041 tgattgtcca cagtacccac aattctcagg acatatacca cttttagcat ttaaaatcat
     5101 gtttaatttt acttttttac caaaataatg ttttcttaaa atgtacgcct catttaataa
     5161 atctaaggta tcaatattag tatcctcata aattttcaat acagtctctt ttgttaattg
     5221 ttccccttgt aatatgcgtt tagccaaatt catattaaca cttcctatct aaaatgttat
     5281 gtactgcctt tttaaaaata cgtaccatcg ttttcacttc tttatttgac atacttaaca
     5341 ctggcacaaa cgtaatgaca ttttctaaat ttctaatcat tagcccgttt tctttacatt
     5401 tacgtacaat acgttcaact ttttcaattt ctaacggcgt tttactatct ttatctgtaa
     5461 ctaattcaac tccaaacatt aagcctcgtc ctcttacatc accaacattt cgatgacgct
     5521 tcagcgcatg taactgtttc tcaagtgttg atgatgtcgt ttcaatatgt gacaataact
     5581 tacgtttttc ataaagtctt atattttcta atgcaaccgt acaaacgatt tgatttccgg
     5641 tgtatgtatg accatggaaa aaggtattaa caccatgcga atcacttaaa aatgtattgt
     5701 atattttttg agatgtcaat gtagctgcaa gtggtaagta gccaccagta atcgccttac
     5761 ctaaacacat aatatccggt tgaacatctt catgattgca tgcaaacatc tttccagttc
     5821 tcccaaaacc aactgctacc tcatcacaaa ttaataagac atcgtatttt ttgcacaatt
     5881 tctcgacttc tttcaaaaag cctttaggat gaacaaacaa gcctgtcgca ccttgaatca
     5941 acggttccaa tataaaccct gcgatttcat catttctttc aattagaatt tgctctatat
     6001 tcgttaaaat agctgtcata atttcatttt cagtatcgta attactgcga tagaaagatg
     6061 ggctttctac ttgaatattc tcaaatatta agtctttaaa tattttatga aaggtcttga
     6121 taccaccaac acttaccgca ccaatcgtat ccccatgata accgtgattt agcgttataa
     6181 acttgttttt cttggcatat ttttctctat caatattttt ccaatactga tatgccatct
     6241 ttattgcgat ttcaacagac gcactgcctg tatcagaata aaatactttt cttagattac
     6301 ttggcgtgat ttcgattaat ttttccgcaa gttctattga cggaatattt gatgatccta
     6361 gcagcgtaga atgggcaatt ttattgagtt gctttttaat taccttattc aagtatttgt
     6421 tattatgacc atgcacattg acccacaacg atgcataacc atctaaatat ttattgccat
     6481 tcgtatcgta aaggtaacta ccctttcctt tttcaatgat gattgcttct tctttgctat
     6541 atacacccat ttgtgtaaat ggatgccaaa catattctga gtctttttgt ttaagttgtt
     6601 gtgtgtaatt catttttaac tcctattaat tgttttaaaa atgcttctga gaaattttca
     6661 tacgtggcat gttcttcaaa tgtatagact gttttatttg ttaattttcc aatcgtaatt
     6721 tgattgtctt tttcaatata gctgtctgta tagcgattca ttattaaaaa attactcgcc
     6781 gatacattct tattaacata atcttggtga acaacggcat cgctaatagc acctaatttt
     6841 gatggcaaca cactgatgac acaatctgca caatcattga ttagatcttt agtcatgtag
     6901 aaatcatctg taccttcata tattggtacg gcaattcccc cagcaccctc aattaagata
     6961 aaatcaaatt ccttatctaa aacttttacc ttatctaata cacgctgctt atttagaaaa
     7021 atttgatctg tcattttaaa tgcaaggtgt ggtgatacag gttgcttaaa agtataaagt
     7081 gacgttatgt catagcttaa atcacattca tttttaaata cttctaaatc tggaaatgtc
     7141 ccgtcttggc gttcctcagt ttgaaatggt ttaaaaacac aaacacgatg accacgtgtt
     7201 ttcaaagcat ggtataaatg ctttgtaaca taggttttgc ctacatcagt attcgtactt
     7261 gtaataaaaa tcctcataaa aaataagcac ctcaatccga atgtaaactt ttataattaa
     7321 taagtttaca tttaaggcgc atgtttttca agaaaacatg aatttaaact tctcagtata
     7381 actataattt ttggcaaaat tttgaccttg ctattgaaat gactaaggct tcttacgaaa
     7441 tttgggcaga tataaacagc gattcgttaa aatatgtcag ttaaaattaa taattgcatt
     7501 atttcttaac aaaaagacat cctagcttta ccatgttgtg aaacaatcca ttaattgtaa
     7561 tattacgttg agcgccttgt tcatcaatgc tataattagt tacataataa attgaacatc
     7621 taaatatacc aaatcccctc actattgccc tagtgagggg atttattttg ctgttgatta
     7681 tctatcaccc tttttatttc caaattctga ttccatatta aactattatt tataatttcc
     7741 agcctgctga tttacttttt attcttataa agtcttgata cattcctggt tttcgaatta
     7801 attcatcatg actacctttt tgaattattt caccttcatt gagcactata atctgatctg
     7861 catttttaat agtttcaagt ttatgtgcaa ttgtaattac tgttttgcct ttacttaatt
     7921 cattaattgc cgtttgaatc agctgttcat tttcagggtc aatacttgca gttgcttcat
     7981 ctaaaataat tattggtgca tcttttaata tcgccctagc aatagaaatc ctttgctttt
     8041 ctccgccaga taaattacta cctttttcat ttagcattgt ttgatatccc tcaggtaatg
     8101 acatgatgaa gtcgtggcaa catgcttgct tcgctgcacg aataatttct tctttcgttg
     8161 cacctggatt gccaaacaat atgttatttt caatcgtatc attaaataaa tacacctttt
     8221 gaaatactgc actaattttc gacattaacg tacttaatgt catatcttta atatcaacac
     8281 catcgatgcg aatatttcca tcatcgatat cataaaagcg caagagtaag tgacataatg
     8341 tagatttccc acttcctgaa ggtccaatta ttgctgttga tgtttgtgta ggtatctcaa
     8401 aattcacatt ctttatcact tgtttatcat catatgaaaa gttcacgttt tgaaaagcga
     8461 tattttcatt tttaatcgtc aaattttttc cattctcatc cagttctgga gcatttttta
     8521 tcttttcaat atcatctatc gtcatatcta tcatttcaag tacgtgtgca gcactattaa
     8581 tattttcaac actatcaaat atcacaaatg aaaaaattga aatcataata agggtaggta
     8641 aatcaatact atgattcata tataataagc atgcaactaa tacaatcatt attgaaacaa
     8701 cttttaaact aagtaaatgc aataaattga atgggatata ttgcatttct atttttgtat
     8761 ttatacgttt actttcattg actgcttggt taaaactttt aagactcgta ttttctttcg
     8821 cgaatgattt tattacttga ataccacgaa taacctccaa taccttttcc actaattggt
     8881 tttgtacatt atgatacgct ggcgcatttt gtcgactctt tctttctaat aattgaatcg
     8941 caaaaaatga tagtaatacg ccaatgcatg ctaataatga tacttgccac gaaactacaa
     9001 gtagagacaa tatgagtact gtaattaata tgtatccatt aacaacaata tctaccattt
     9061 tcatggcaaa gttttctaga aatgttaaat ctgttgttac tattgttgtt aactcatttg
     9121 aatgatgcga attaaaataa cctaacctta catttttcaa tttatcccct atatctaaac
     9181 gttctttcgc actcatttca taagcgatgc tctcatggct tttgcttttg aaatatgctg
     9241 taataaatcg tccaatcact aataaaacca tgataattac aacattcaat atatctttca
     9301 tataaatagg tttatgagat agcacattat taaatatttt tgcagctaaa aagataggta
     9361 acgcaataaa aatagcattt aaaaatgaca tgctaaatcc taaaatcatt cttgctttat
     9421 atggtcttat ccagtttaaa attttaaatg taatttgaaa catatttgac ctccttttct
     9481 cattagttta attatgtcca gtattgattc cccaatcttt tgtatgcatg tgcgtatccc
     9541 acattttctt ataattaccg tttaatttta gcaataagtg atgtgaccct ttttctaaaa
     9601 tttgttgttt acctaaaaca ataatttgat ctgcatgttg aatcgtagat aaccgatgtg
     9661 caataacaat caatgtttta tcctgcgtca acacatttaa tgcttcttga attttttgtt
     9721 cgttgtctgg atcaacgtaa gcagtcgctt catctaatac aatgataggc gcatctttta
     9781 atatcattct tgcaatagtg actcgttgtt tttcaccacc agacaattta tctcccactg
     9841 taccaacatt cgtatcatat ccatctggca acttttcaat aaattcatga cattgtgcta
     9901 acttggcagc tttttcaact gcctcatccg tagcttctgg attgccaagt ttaatatttt
     9961 ctttaaaagt aagatttaat aaaaagttat cttgtccaac aaaaccaact aaatcgttaa
    10021 gttgtttcga ttcaatatct tttatattta taccgccaat cgtaatttca cctgaagtca
    10081 catcccagta tcgtgatata agcttggcaa tggttgactt accgctacca gaagccccga
    10141 cgatagctgt gaaattattt tctggtactg taaatgataa atgcttaaag accagatcgt
    10201 ccttatcttt gttatatgaa aatccaacat tattaaaagc aatttcataa tgttgaggct
    10261 tcttaaattt tgttgacaat actaactctt ctagacttaa tatttgattc acttcagtta
    10321 atgcgtattg tatagacttt aaatgattta cataattagt aaaattctta atcggtgcta
    10381 ctacacctaa agataatacg atgcatagga aaaattccgc atagtttaat tggttgatag
    10441 atatcaaata catgccgacc ggtaaaatcc ctaaaaatgt tgaaggtaat acactagctc
    10501 ctaaattcat atacccccat gtatttttaa accaattcaa agtgtgaatc ttataattat
    10561 ctactgcatc tttatatttt ttatatgaac tttgagattg attaaatgtt ttaatcactt
    10621 caatgccttc gataaactct acaatcgcgc tattcatata attatttgat ttcatttgtt
    10681 cagcatatgt ctcattaaat ccagacataa cttttttaaa agcgaaaatt gaaattggta
    10741 tcgttactaa taaggcacta gccatacgcc aatcaatgag cattatgtat aaaaagatag
    10801 cagctgacaa aagtaagttt cctataactt caggaatcat atgtgctaaa ggtaattcta
    10861 ttgtttcaac cttatcgaca aatatatttt ttaattcacc tattttctta gattccacta
    10921 cgcctaaagg gaggcgcatt aatttttgag ctaatttttt acgaatttca gataaaattt
    10981 catatgccgt aatatgtgat agcatcgttg acgctccaaa acaacacact tgtgaaatat
    11041 aagtgattaa agcaataaag atataaacca taatcgaatt aatcgtatat gcattgttaa
    11101 tcatcattaa aataatttta aagactgccc aatatggaac taatccagaa aagacactga
    11161 tgatagacaa caaaattgat aacataattt tccatttact atcagaagca aatccgctga
    11221 gtatctttat ccaatttgtc tgattgttca tcgtatatgc aactcctttc aattaataat
    11281 ctaaattaag ccgcttatat tatttatttc actggatgat atacataata taaatttgtc
    11341 atttgttaaa aattaatact tattacaagt acatcatata ttagttgata acgattatca
    11401 atgtcgcgtg gatttgtgac acatttcttt taaaaattca caaggttatg gggcagaaat
    11461 gataaagagc cactaatgat ttattatgta atgattcttt atccttagcc acagctaata
    11521 tgtacttaaa aataggaata catgagtaaa actctatgtt ggggctccgc caacttgcac
    11581 attattgtaa gctgactttc cgccagctac tgtgttgggg ccccgccaac ttgcattgtc
    11641 tgtagaaact gtggatccaa attctctatg ttgggtccct gagtatagcc ttgtagagtc
    11701 tagtacattg atttgtatcc caatgtccct ataattgatt attcgcttta tctaatgatc
    11761 ctatgactca actattaaat catttttcga aatacttaat tctaatataa ttaaattcat
    11821 ttattgtaat attgcaaaat acattgcaca ccttgtttat caatgctata attaattaca
    11881 taataaattg aacatctaaa tacaccaaat cccctcacta ctgccatagt gaggggattt
    11941 atttaggtgt tggttatttg tcaccttttt tattgttgcg cgttcgtaac caatgtgcaa
    12001 aaaacgcaac aagacagccg cttaaagctg aagtcatgat gttaattaat aaattgaaca
    12061 tccatcatac acctcctctc tgcgttaaag taacgcccga gatgttaggc gaccattata
    12121 ttatatcatt tatttattat atttcacgca atattaaagt tcgagtaaag cgttttttag
    12181 tggtttacgc tactttaatt actatctttt aaaatccatt tagataatac aaatgtgatg
    12241 ggtatcgtaa tcactaaacc tgcaaatggc gcaatttccg ctggcaaatt tagccaggat
    12301 acaaaaacat ataataaaac tgtttgtaag cttacgttga caatctgcgt aattggaaaa
    12361 ctaatgaatt ttctccaagt aggttttacc ctgtaaacaa aataacaatt caaataatac
    12421 gaaatcacaa aagcgactag aaatccggta atatgactaa tcatatattc aatgtgtaat
    12481 aattttaaca gcaataaata gacaacataa taatttaacg tattaatgcc gccaacaatg
    12541 ataaatttta aaatttcagc atgcgtttgt gttagtttca tatgtgtaac tcctcaacat
    12601 caaaatatat gcatagctac gttctccaac atactcgaat atgcgagcca atccgcttca
    12661 cttcaaattt acttatttca acctttatac cctttcacag caaatttagt ctctttcctc
    12721 tcatccttat acgccattat aatgtaactg atttatcgcg tgactcatta gcactataga
    12781 gattacttta gttcactagt aattttatat acaataagag cgacaacagt aatgagagga
    12841 tgtctactat gcaattacaa aaaattgtca tcgctcctga ctcatttaag gaaagtatga
    12901 ccgcacagca agttggcaat attataaaac aggcatttac taatgtttat gggaataccc
    12961 ttcattatga tatcattccg atggctgatg gtggtgaagg taccacagat gctttaatgc
    13021 atgcaacagg tgccactaag tatacagtca tcgttaatga ccctttaatg cgacctattg
    13081 aagcatgtta tgcacgcgca gacgaacaac aaattgcaat tattgaaatg gcggcagcgt
    13141 caggtttgga tttattagaa aaagaggaac gtaatccttt atacacatca tcatatggta
    13201 ccggtgaact aattaaagat gcattaaatc atggtgctaa gaccattatt ttagggattg
    13261 gtggcagtgc aacaaatgat ggtggtacag gtatgctaag tgcactaggc gtaaagttta
    13321 ctgatgtaaa cggggactta ttacaaatga atggtgctaa tcttgctcac attgcacaaa
    13381 tcgatataac caatctagat tcgcgattaa aagaggtgac ctttaaagtg gcctgtgatg
    13441 tttcaaatcc tttattgggt gaaaatggtg ctacctatat ttatggtcct caaaaaggcg
    13501 ctgatgcaaa gatgatacca aagttggatt tcgcaatgtc gcattatcat gataagataa
    13561 aaatgtgcac aggaaagtcc gttaatcaaa taccaggttc tggtgcagct ggcggtatgg
    13621 gcgcagcatt attagcgttt tgtgagacaa ctttaacaaa aggtattgat gtcgtctttg
    13681 acattacaga ttttcatcaa agaattaaag atgcagacct cgttattact ggagaaggac
    13741 gcatggatta tcagaccatc tttggtaaaa cacccgtagg cgttgcgtta gctgcaaaac
    13801 aatatcatat tcctgtcatc gcgatttgtg gcagtctagg cgaaaattat caacatgttt
    13861 acgatttcgg tattgatagt gcctattcta taatctcttc acctagcact ttagaagatg
    13921 tcctacaaaa tagcgaacaa aatttattaa acactgcaac tgacattgct cgtattctga
    13981 aattacaata atgtcaaagt aaatcatcag ctttattatt tgcagttaaa acttgaatga
    14041 ggtgaaaccc atgaaaagaa ctgataaata ccgtgattca tatcaatacg acaatcaaaa
    14101 ccaaaatcat cgtcgtcaat ctgaagacgc atcgtataga caacaatatg ctaaaggcga
    14161 tcctgaagaa cacccggaac gatactataa tggtagagat tatcgaagag aacaaattct
    14221 tgaagaagaa aacgagaaat cccgccgttc aaaaaaatgg ttatatatca ttattgccat
    14281 tctcttaatt attgtcgcta tttttgtcac acgcgcctta cttaacaatg atagcgataa
    14341 agttagtaat gaccctaaag tctctcaaaa ttataaaaaa caagttgaaa atcaagacgg
    14401 ccaaattaac cagcaagtag ataatgctaa agaaaatatt aaaaacaacc aaaaaactga
    14461 tgacattatt aaaaatttac aaaatcaaat cgacaacttg aagcagcaag aacaaaacaa
    14521 agctgattct aagctaactc aattttatca agaccaaatc aacaaattga cagaggcaaa
    14581 taatgcactt aaaaacaatg caagccaagg taaaattgaa agcatgttaa atgatattaa
    14641 tacaaaattc gacagtatta aatctaaatt agaaagctta tttaaagatg acaatggtgg
    14701 cgctaattaa ttattacacc tgctttgatg ataaacatta attccctata ctttatctgt
    14761 atcactacgt tattcgtgat gatgcattaa gagtataggg attttttata taaacttgta
    14821 ttctaactac at
//
LOCUS       DANBWH010000051        14780 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.053, whole genome shotgun sequence.
ACCESSION   DANBWH010000051 DANBWH010000000
VERSION     DANBWH010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14780
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.053"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            19..909
                     /locus_tag="PPY92_001066"
     CDS             19..909
                     /locus_tag="PPY92_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HDJ2625082.1"
                     /translation="MNKISKYIAIASLSVAVTVSAPQTTNSTAFAKSSAEVQQTQQAS
                     IPASQKANLGNQNIMAVAWYQNSAEAKALYLQGYNSAKTQLDKEIKKNKGKHKLAIAL
                     DLDETVLDNSPYQGYASIHNKPFPEGWHEWVQAAKAKPVYGAKEFLKYADKKGVDIYY
                     ISDRDKEKDLKATQKNLKQQGIPQAKKSHILLKGKDDKSKESRRQMVQKDHKLVMLFG
                     DNLLDFTDPKEATAESREALIEKHKDDFGKKYIIFPNPMYGSWEATIYNNNYKASDKA
                     KDKLRKNAIKQFDPKTGEVK"
     gene            1158..2207
                     /locus_tag="PPY92_001067"
     CDS             1158..2207
                     /locus_tag="PPY92_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDJ2625083.1"
                     /translation="MFLAWNEIRRNKLKFGLIIGVLTMISYLLFLLSGLANGLINMNK
                     EGIDKWQADAIVLNKDANQTVQQSVFNKKDIENKYKKQATLKQTGEIVSNGHQKDNVL
                     VFGVEKSSFLVPSLIEGHKATKDNEVLADETLKNKGFKIGDTLSLSQSDEKLHIVGFT
                     ESAKYNASPVIFTNDATIAKINPRLTGDKINAVVVRDTNWKDKKLNQELEAVSINDFI
                     ENLPGYKPQNLTLNFMISFLFVISATVIGIFLYVMTLQKTSLFGILKAQGFTNGYLAN
                     VVISQTLILALFGTAFGLLLTGVTGAFLPDAVPVKFDVLTLLVFAIVLMIVSVLGSLF
                     SILTIRKIDPLKAIG"
     gene            2220..2897
                     /locus_tag="PPY92_001068"
     CDS             2220..2897
                     /locus_tag="PPY92_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDJ2625084.1"
                     /translation="MLKFENVTKSFKDGNRNIEAVKDTNFEINKGDIIALVGPSGSGK
                     STFLTMAGALQTPTSGHILINNQDITTMKQKALAKVRMSEIGFILQATNLVPFLTVKQ
                     QFTLLKKKNKNVMSNEDYQQLMSQLGLTSLLNKLPSEISGGQKQRVAIAKALYTNPSI
                     ILADEPTAALDTENAIEVIKILRDQAKKRKKACIIVTHDERLKAYCDRSYHMKDGVLN
                     LENETVE"
     gene            3105..4136
                     /locus_tag="PPY92_001069"
     CDS             3105..4136
                     /locus_tag="PPY92_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDJ2625085.1"
                     /translation="MDLMIGTLFLFLVLVIFTLFTYKAPNGMRAMGALANAAIATFLV
                     EAFNKYVGGEVFGIKFLEELGDAAGGLGGVAAAGLTALAIGVSPVYALVIAAACGGMD
                     LLPGFFAGYMIGYVMKYTEKYVPDGVDLIGSIVILAPLARLIAVLLTPVVNSTLIRIG
                     DIIQSSTNTNPIIMGIILGGIITVVGTAPLSSMALTALLGLTGVPMAIGAMAAFSSAF
                     MNGTLFHRLKLGDRKSTIAVSIEPLSQADIVSANPIPIYITNFFGGAIAGLIIAMSGL
                     INDATGTATPIAGFLVMFGFNHPMTIVIYGVVMAIVGALAGYLGSIVFKKYPIVTKQD
                     MINRGAVDA"
     gene            4479..5597
                     /locus_tag="PPY92_001070"
     CDS             4479..5597
                     /locus_tag="PPY92_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDJ2625086.1"
                     /translation="MSDSEKEILKRIKDNPFISQRELAEAIGLSRPSVANIISGLIQK
                     EYVMGKAYVLNEDYPIVCIGAANVDRKFYVHKDLVAETSNPVTSTRSIGGVARNIAEN
                     LGRLGETVAFLSASGQDSEWEMIKRLSTPFMNLDHVQQFENASTGSYTALISKEGDMT
                     YGLADMEVFDYITPEFLIKRSHLLKKAKCIIVDLNLGKEALNFLCAYTTKHQIKLVIT
                     TVSSPKMKNMPDSLHAIDWIITNKDETETYLNLKIESTDDLKIAAKRWNDLGVKNVIV
                     TNGVKELIYRSGEEEIIKSVMPSNSVKDVTGAGDSFCAAVVYSWLNGMSTEDILIAGM
                     VNAKKTIETKYTVRQNLDQQQLYHDMEDYKNGKFTKVY"
     gene            5572..6495
                     /locus_tag="PPY92_001071"
     CDS             5572..6495
                     /locus_tag="PPY92_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HDJ2625087.1"
                     /translation="MANLQKYIEYSREVQQARENNQPIVALESTIISHGMPYPQNVEM
                     ATTVEQIIRNNGAIPATIAIIDGKIKIGLESEDLEILATSKDVAKVSRRDLAEVVAMK
                     CVGATTVATTMICAAMAGIQFFVTGGIGGVHKGAEHTMDISADLEELSKTNVTVICAG
                     AKSILDLPKTMEYLETKGVPVIGYQTNELPAFFTRESGVKLTSSVETPERLADIHLTK
                     QQLNLEGGIVVANPIPYEHALSKAYIEAIINEAVVEAENQGIKGKDATPFLLGKIVEK
                     TNGKSLAANIKLVENNAALGAKIAVAVNKLL"
     gene            6506..7726
                     /locus_tag="PPY92_001072"
     CDS             6506..7726
                     /locus_tag="PPY92_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDJ2625088.1"
                     /translation="MNILFAITGIAFALFVAFLFSFDRKNIDFKKTLIMIFIQVLIVL
                     FMMNTTIGLTILTALGSFFEGLINVSKAGINFVFGDIQNKNGFTFFLNVLLPLVFISV
                     LIGIFNYIKVLPFIIKYVGIAINKITRMGRLESYFAISTAMFGQPEVYLTIKDIIPRL
                     SRAKLYTIATSGMSAVSMAMLGSYMQMIEPKFVVTAVMLNIFSALIIASVINPYKSDD
                     SDVEIDNLTKSTETKSVNGKTGKPKKVAFFQMIGDSAMDGFKIAVVVAVMLLAFISLM
                     EAINIMFGSVGLNFKQLIGYVFAPIAFLMGIPWSEAVPAGSLMATKLITNEFVAMLDF
                     KNVLGDVSARTQGIISVYLVSFANFGTVGIIVGSIKGISDKQGEKVSSFAMRLLLGST
                     LASIISGSIIGLVL"
     gene            complement(7831..9363)
                     /locus_tag="PPY92_001073"
     CDS             complement(7831..9363)
                     /locus_tag="PPY92_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HDJ2625089.1"
                     /translation="MKEVGFGTLNWVAVIIYLLAMLFIGVYFTKRASQSTNSFFTASG
                     RLPSWVVGFSIYATTLSAITFMSTPEKAFLTDWSYIAGNIAIVAIIPLLIYFYVPFFK
                     KLKVTSAYEYLEARFGPSIRVIGSLLFVVYHLGRVAIVIYLPTLAITSVSDMNPYIVA
                     SLVGLLCILYTFLGGFEGVVWSDFIQGVILLGGALVIIILGVMNIKGGFGTVFADAIE
                     HKKLISADNWKLNTAAAAIPIIFLGNIFNNLYQYTASQDVVQRYQASDSLKETNKSLW
                     TNGILALISAPLFYGMGTMLYSFYTHEAVLPKGFNTSSVVPYFILTEMPPFVAGLLIA
                     AIFAAAQSTISSSLNSISACISIDIKQRFFGKDSERHEVNFARFIIIIAGIFGFGMSL
                     YLIASNSNDLWDLFLFVTGLFGVPLAGVFAVGIFTKRTNTFGVICGLILGIIFAYVYN
                     GVGKGNSPFYVSTISFTVAFVFAYILSFIVPSKHKKDITGLTIFEKDKPSTYISKTAT
                     NK"
     gene            complement(9403..10284)
                     /locus_tag="PPY92_001074"
     CDS             complement(9403..10284)
                     /locus_tag="PPY92_001074"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDJ2625090.1"
                     /translation="MNKDLKGLYAALLVPFDENGQVNEQGLKQIAQNAIETEELDGLY
                     VNGSSGENFLLNTEQKKQVFKVAKEAVGDKVKLIAQVGSLDLNEAIELGKYATELGYD
                     ALSAVTPFYYPFTFEEIRDYYFDIIEATQNNMIIYAIPDLTGVNISIEQFSELFNHEK
                     IVGVKYTAPNFFLLERIRKAFPDKLILSGFDEMLVQATISGVDGAIGSTYNVNGRRAR
                     KIFDLARQGQIQEAYQLQHDSNDIIETVLSMGIYPTLKEILRHRDIDAGLPKRPFKPF
                     NEAHRQTLDQLIAKYDL"
     gene            10442..11302
                     /locus_tag="PPY92_001075"
     CDS             10442..11302
                     /locus_tag="PPY92_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDJ2625091.1"
                     /translation="MYYIAIDIGGTQIKSAVIDKQLNMFDYQQISTPDNKSELITDKV
                     YEIVTGYMKQYQLIQPVIGISSAGVVDEQKGEIGYAGPTIPNYKGTNFKRLLKSLSPY
                     VKVKNDVNAALLGELKLHQYQAERIFCMTLGTGIGGAYKNNQGHIDNGELHKANEVGY
                     LLYRPTENTTFEQRAATSALKKRMIDGGFTRSTHVPVLFEAAEEGDDIAKQILNEWAE
                     DVAEGIAQIQVMYDPGLILIGGGISEQGDNLIKYIEPKVAHYLPKDYVYAPIQTTKSK
                     NDAALYGCLQ"
     gene            complement(11579..12379)
                     /locus_tag="PPY92_001076"
     CDS             complement(11579..12379)
                     /locus_tag="PPY92_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDJ2625092.1"
                     /translation="MKFENRVQRYQHLFTKTDKRIVNYIRQNGYSDAFSTINSLAHAI
                     GTSPATMTRFSHKLDYENFQDLKFNIQQEMTETVIENSPIIQRIHKYHQQIIQQTGEF
                     IDNDIIQTFIDKLQSSRHILFAGLGSSGLSATEFYYRMIRMGLKGNVTTDSHLMKISA
                     SLLSHSDMFIAMSNSGNTSELISAAEVAKSHGAYVVAITNFEGSKLTDCADLVLLTTD
                     QSRNTDHQFINTQIATLFLIDIVSYHLLENTNLSQTYQHTKSIILDNK"
     gene            12519..13187
                     /locus_tag="PPY92_001077"
     CDS             12519..13187
                     /locus_tag="PPY92_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDJ2625093.1"
                     /translation="MLPHGLIVSCQALADEPLHSSFIMSKMALAAYEGGAVGIRANTK
                     EDILAIKETVDLPVIGIVKRDYDHSDVFITATSKEVDELIESQCEVIALDATLQQRPK
                     ETLDELVSYIRTHAPNVEIMADIATVEEAKNAARLGFDYIGTTLHGYTSYTQGQLLYQ
                     NDFQFLKDVLQSVDAKVIAEGNVITPDMYKRVMDLGVHCSVVGGAITRPKEITKRFVQ
                     VMED"
     gene            complement(13318..14631)
                     /locus_tag="PPY92_001078"
     CDS             complement(13318..14631)
                     /locus_tag="PPY92_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HDJ2625094.1"
                     /translation="MNQYHSNAQQPSAWRFFVYSLVGILCFFIPFTINGNNTIFVDHV
                     HLAIRSIIGPLMPYVALIMILIGTALPIVRRTFMTSITNLVITLFKVAGAMIGIMYVF
                     KIGPSILFKANYGPFLFEKLMMPLSILIPVGAIALSLLVGYGLLEFVGVYMEPIMRPI
                     FKTPGKSAVDAVASFVGSYSLGLLITNRVYKQGMYNKREATIIATGFSTVSATFMIIV
                     AKTLGLMPHWNLYFWITLVITFVVTAITAWLPPITNESTEYYNGQEGEQEVAIEGSRL
                     KTAYAEAMKQNALTPSLVKNVWDNFKDGLEMTVGILPSILSIGFLGLIVANYTPFIDW
                     LGYIFYPFIYIFPIADQAVLAKASAISIVEMFLPSLLVTKAAMSTKFVVGVVSVSAII
                     FFSALVPCILATEIKIPVWKLIIIWFLRVALSLLITIPVALLIFG"
BASE COUNT         5100 a         2277 c         2826 g         4577 t
ORIGIN      
        1 taatcatgag gtgataagat gaataaaatt tcaaagtata ttgcaatagc atcattatcg
       61 gtagcggtta cagtttcagc accacaaacg acaaattcta cagcgtttgc caaaagttct
      121 gctgaagttc aacaaacgca acaagcttct ataccagcat cacaaaaggc gaatcttggt
      181 aatcaaaata ttatggcagt ggcttggtat caaaattcag ctgaagcaaa agcattatat
      241 ttacaaggtt ataacagtgc aaagacacag ttagataaag agattaaaaa gaataaaggt
      301 aaacataagt tagctattgc tttggattta gatgaaacag ttttagataa ttctccatat
      361 caaggctatg catcaataca taataaacct ttcccagaag gttggcatga atgggtacaa
      421 gctgctaaag ctaaacctgt ctatggcgca aaagaattct tgaaatatgc tgacaaaaaa
      481 ggtgtcgata tctactatat ttctgataga gataaagaaa aagatttaaa ggcaacacaa
      541 aagaacttaa aacaacaagg tatccctcaa gctaagaaga gtcatatttt actaaaaggt
      601 aaagatgata agagtaaaga atcacgcaga caaatggttc aaaaggatca taaacttgtc
      661 atgctatttg gagataattt attagacttt acagatccaa aagaagctac agctgaatct
      721 cgtgaagcat taattgaaaa acataaagac gatttcggta agaaatatat cattttccct
      781 aacccaatgt atggtagttg ggaagctacg atttacaaca ataactataa agcaagtgac
      841 aaagcaaaag ataaattacg taaaaatgct attaagcaat tcgatcctaa aacaggcgaa
      901 gttaaataat atatgaattg gacgtctaca tgtacttaaa ggtatatgta ggcgttttta
      961 aattgatcct taattttaaa tgtaccgtac aaaaataata gacaattaat tgcattttcc
     1021 ataaatttgc ttttaatata aaaagttcgg gtataatgaa ggataagtgt atgtataaca
     1081 ttacagagcg caacacgacg tacgtgcatc taagaatata aacaatgttt aaatcatagt
     1141 agtagggaga gaaatgcatg tttttagctt ggaatgaaat acggcgcaac aaattgaagt
     1201 ttggactaat tattggtgtg ttaacgatga ttagttactt gctattttta ttatctggat
     1261 tggcgaatgg tcttatcaat atgaataaag aaggcattga taagtggcaa gcagatgcca
     1321 ttgttctaaa taaagatgcc aatcaaactg tgcaacaatc tgtttttaac aagaaagata
     1381 ttgaaaataa atacaagaag caagctactt tgaagcaaac aggggaaatt gtgtctaatg
     1441 gccatcaaaa agacaatgtt ttagtgttcg gtgttgaaaa gtcatcattt ttagttccga
     1501 gtttaataga agggcataaa gcgactaaag ataatgaagt gttagctgat gaaacgctta
     1561 aaaataaagg atttaaaatt ggtgacacat tatcactatc tcaatcagat gaaaaattgc
     1621 atatcgtagg ttttacagaa agtgcaaaat ataatgcgtc accagtcatt ttcacgaatg
     1681 acgctaccat tgccaagatc aatcctagat tgactggaga taaaattaat gcagttgttg
     1741 tacgtgatac aaattggaaa gacaaaaaat taaaccaaga gcttgaagcg gtaagtatta
     1801 atgactttat tgaaaattta ccaggttata aaccacagaa cttaacatta aactttatga
     1861 tttcattctt atttgtcatt tcagctacag ttataggcat tttcttatat gtcatgacat
     1921 tacaaaaaac aagtttattt ggcatattaa aggctcaagg atttacgaat ggctatttag
     1981 cgaacgtagt tatttcgcag acgctcatat tagcattatt tggtacggca tttggcttac
     2041 tgttaacagg cgttacaggt gcatttttac ctgatgcagt acctgtcaaa ttcgatgtac
     2101 taacattgct cgtatttgca attgtgttaa tgattgtctc tgtattagga agtttattct
     2161 ccattttaac aattagaaaa atagatccgt taaaggcgat tgggtaggag gtgtagcaaa
     2221 tgttgaaatt tgaaaatgta acaaagtcat ttaaagatgg gaatcgtaac attgaagcgg
     2281 ttaaagatac aaattttgag ataaataaag gtgatattat agcgttggtt ggaccttctg
     2341 gctctggtaa aagtacattt ctaactatgg caggtgcttt acaaacgccg acatctgggc
     2401 acattttaat caataaccaa gatattacga caatgaagca aaaagcattg gcaaaagtta
     2461 gaatgtctga aataggtttt attttacaag ctacaaacct tgtaccattt ttaacggtaa
     2521 agcaacaatt tacattattg aaaaagaaaa ataagaatgt tatgtctaat gaagactatc
     2581 agcaacttat gtcacaatta ggtctaactt cattgcttaa taagttacct tcagaaattt
     2641 caggtggtca gaaacaacgt gtggcgatag ccaaagcgtt atatacgaat ccgtcgatta
     2701 ttttagcgga tgaacctacc gcggcgttag atactgaaaa tgcgattgaa gtcattaaaa
     2761 ttctacgtga tcaagccaaa aaaagaaaga aagcatgtat tattgttaca catgatgaac
     2821 gacttaaagc atattgtgat cgttcatatc atatgaaaga tggcgtcctt aatcttgaaa
     2881 atgaaacagt agaatagttt tattaagccg gtacatcatg tgccggtatt tttatgttta
     2941 tgtattattt gaataaactt tcacattcaa ttaataataa ttattatcga aaattagaaa
     3001 tattccgtga aatataatat tttttgtagt aaaatggcct ctaagtattc aatatttaaa
     3061 tatggggatt gaatataaaa ttatcgtaat gggggtcaat ggttatggat ttaatgatag
     3121 gtactttatt tttatttttg gtcttagtga tttttacatt atttacatat aaagcaccta
     3181 atggtatgcg tgccatggga gcattagcta atgcagcaat cgcaacattt ttagtggaag
     3241 catttaataa atatgttggt ggcgaagtat tcggtattaa atttttagaa gagctaggag
     3301 acgctgcggg aggtctaggt ggtgtcgctg ccgctggatt aacagcatta gctatcggtg
     3361 tatcaccagt atatgcatta gttatagcag ccgcgtgcgg tggtatggat ttattaccag
     3421 gtttctttgc gggttatatg attggatatg tgatgaaata tacagagaaa tatgtgccgg
     3481 atggtgtcga cttaattgga tcgattgtca tcttagcgcc attagctcgt cttattgcag
     3541 tattattaac gccagtagtg aatagtacat tgattcgaat tggtgatatt atccaaagta
     3601 gtacgaatac gaatccaatt atcatgggta tcattttagg tggtattatc acggttgtcg
     3661 gcacagcgcc attgagttca atggcattga cagcattatt aggtttaacg ggtgtaccta
     3721 tggctattgg tgccatggca gcatttagtt cggcatttat gaatgggacg ctattccatc
     3781 gcttaaaatt aggtgatcgt aagtctacga ttgcagtaag tattgaacct ttatcacaag
     3841 cagatattgt atcagccaat ccaattccaa tctatattac aaatttcttt ggtggtgcga
     3901 ttgctggttt aattattgct atgtcaggtt taattaacga tgcgacaggt acagctacac
     3961 cgattgcagg atttttagta atgtttggat ttaatcatcc aatgactatt gtaatttatg
     4021 gtgtagtaat ggcgattgta ggtgcgcttg caggttatct tggttcaatc gtatttaaaa
     4081 aatatccaat tgttactaag caagacatga ttaatcgagg tgcagttgac gcatagcatc
     4141 atcatattga atagtaaaaa caaataaaac atagtaacgt gattcagtcg atgaaaaaat
     4201 cgataatgag tcacgttttt ttatagaaaa atataagaca taaaaatgtc ataatttatt
     4261 gtcgacaaat atcatactgt ataaacattt atcattttct caagtacctt ttacacgatg
     4321 gaatgaactt actttttacg aaattatgcg tattttataa acaaatatca ttgatataac
     4381 ggcaaatgta agcgtttaca acagaaataa cagcatgcta cgatattttt gtaaattcac
     4441 tgattcaagt attttaagtc aatatgagga gggatgttat gagcgattct gagaaagaaa
     4501 ttttaaaaag aattaaagat aatccgttta tttcacaacg tgaacttgct gaggcaattg
     4561 gattatctag acccagcgta gcaaacatta tttcaggatt aatacaaaag gaatatgtta
     4621 tgggaaaggc atatgtttta aatgaagatt atcctattgt ttgtattggc gcagcgaatg
     4681 tagatcgtaa gttttatgtg cataaagatt tagttgcaga aacatcaaat cctgtaacgt
     4741 caacacgctc tattggtggc gtagcaagaa atattgctga gaacttaggt aggcttggcg
     4801 aaacggtcgc ttttttatct gctagtggac aagatagtga atgggaaatg attaaacgat
     4861 tgtccacacc atttatgaat ttggatcatg ttcaacaatt tgaaaatgcg agtacaggtt
     4921 catatacagc tttaattagt aaagaaggcg acatgacata tggcttagca gatatggaag
     4981 tgtttgacta cattacgcct gaatttttaa ttaagcgttc acacttattg aaaaaggcta
     5041 agtgcattat tgtcgatttg aatttaggca aagaggcatt aaacttctta tgtgcctata
     5101 ccacgaaaca tcaaatcaaa ttagttatca ccacggtttc ttccccaaaa atgaaaaata
     5161 tgcctgattc attacatgct attgattgga ttatcacgaa taaagatgaa acagaaacat
     5221 acttaaattt aaaaatagaa tctactgatg atttaaaaat agctgctaaa cgctggaatg
     5281 atttaggtgt taaaaatgtt attgtgacaa atggcgtgaa agaactcatt tatcgaagtg
     5341 gtgaggaaga aatcatcaag tcagttatgc catcaaatag tgtgaaagat gttacaggtg
     5401 caggcgattc attctgtgct gcagtagtat atagctggtt aaatgggatg tctactgaag
     5461 atatattaat tgctggtatg gttaacgcaa agaaaacgat agaaacgaaa tatacagtta
     5521 ggcaaaacct agatcaacag caactttatc acgatatgga ggattataaa aatggcaaat
     5581 ttacaaaagt atattgagta ttctcgagaa gttcagcaag cacgggagaa caatcaaccg
     5641 attgtagcat tagaatcaac aattatttcg catggtatgc cgtacccaca aaatgttgaa
     5701 atggcaacaa cagtagagca aattatcagg aataatggtg ccattccagc aaccatagcc
     5761 attatagatg gcaaaattaa aattggttta gaaagcgaag atttagaaat actggcaact
     5821 agtaaagacg ttgctaaagt atctagaagg gatttagcag aagttgttgc gatgaagtgt
     5881 gttggtgcta ctactgtagc gacgacgatg atatgtgctg caatggctgg tattcaattt
     5941 tttgttacag gaggtattgg gggcgtccat aaaggtgcag aacatacgat ggacatttca
     6001 gcagacttag aagaactgtc taaaacaaat gtcactgtta tctgtgcagg tgccaaatca
     6061 attttagact tacctaagac gatggagtat ttagaaacaa aaggcgttcc agttattgga
     6121 tatcaaacga atgaattgcc agcattcttc actcgcgaaa gcggtgttaa gttaacaagt
     6181 tcggttgaaa cgccagaacg acttgctgac attcatttaa caaaacagca gttaaatctt
     6241 gaaggtggca ttgttgttgc taatccaatt ccatatgagc atgccttatc aaaagcatat
     6301 attgaggcaa tcataaatga agctgttgtt gaagcggaaa atcaaggtat taaaggtaag
     6361 gacgccacac cgttcttgtt agggaaaatt gtagaaaaaa cgaatggtaa aagtttagca
     6421 gcaaatataa aacttgttga aaacaatgcg gcgttgggtg ctaaaattgc tgtcgctgtt
     6481 aataaattat tgtaggtgat gatacatgaa tattttattc gctatcacag ggatagcatt
     6541 tgcactattt gttgcgtttt tattcagttt tgatcgtaaa aacatagact tcaaaaagac
     6601 gttaataatg atatttattc aagtgttgat tgtgttattt atgatgaaca caacgattgg
     6661 tttaacaatc ttaactgcat taggctcatt ttttgaaggg ttaataaatg ttagtaaagc
     6721 aggtataaac tttgtttttg gagatataca aaataaaaat ggctttacgt tctttttaaa
     6781 tgtgttactg ccattagtgt tcatttctgt attaataggc atttttaatt atattaaggt
     6841 attaccattt attatcaaat atgtaggtat cgctattaat aaaataacga gaatggggcg
     6901 cttagaaagt tactttgcta tttcaacagc aatgtttgga caaccagaag tatatttaac
     6961 aataaaagat attattccaa gattatctag agcgaaatta tatacaattg cgacgtctgg
     7021 tatgagtgct gttagtatgg caatgttggg ttcatatatg cagatgattg agcccaagtt
     7081 cgtagttaca gctgtaatgt taaatatttt tagtgcgctt atcatcgcta gtgtaatcaa
     7141 tccctataaa tctgatgata gtgatgttga gattgataat ttaactaaat caacggaaac
     7201 gaaatcagtg aatggaaaaa caggaaaacc taagaaagtt gccttcttcc aaatgatagg
     7261 tgatagtgcg atggatggat ttaaaatcgc tgttgtagta gccgtaatgt tgttagcatt
     7321 tatttcatta atggaagcaa ttaatatcat gtttggtagt gttggtttga actttaaaca
     7381 gcttattggc tatgtgtttg caccaatcgc attcttaatg gggattccat ggagcgaagc
     7441 tgttccagct ggctctttaa tggcgactaa attaattaca aatgagtttg tagcaatgct
     7501 tgattttaaa aatgtcctgg gtgatgtatc tgctcgaaca caaggtatca tttcagttta
     7561 cttagtaagc ttcgctaatt ttggtacggt tggtatcatc gtaggttcaa ttaaaggcat
     7621 tagtgataaa caaggagaaa aagtttcatc ctttgcaatg aggctgttac ttggttcaac
     7681 tctagcttca atcatttcag gatcaatcat tggcttagta ttgtaaatta atcgaagtat
     7741 ctaaattaaa ttcatggcaa agctaaaccc catcaccaag ttggcgcaac agcgcatcat
     7801 aacttggtga tgggatttta tcataacaat ctacttattc gtagccgttt ttgaaatgta
     7861 tgttgatggt ttatcttttt caaaaattgt taatcccgtt atatcttttt tatgttttga
     7921 agggacaatg aagctaagta tataagcaaa gacaaaagca actgtaaatg aaatggtaga
     7981 tacatagaaa ggtgagttac ctttgccaac accattatag acataagcaa agatgatacc
     8041 caatattaat ccacaaataa caccgaatgt attcgtacgt ttagtgaaaa taccaactgc
     8101 aaatacacca gctaatggaa caccgaataa tccagttaca aacaagaata aatcccataa
     8161 gtcatttgaa ttagaagcaa ttaagtatag tgacattcca aaaccgaaaa tacctgcaat
     8221 gatgataatg aaacgtgcaa agttaacttc gtgtcgctcg ctatcttttc cgaagaagcg
     8281 ttgcttaatg tcgattgaaa tacaagcaga tatagaattt aaactagatg aaatggtaga
     8341 ctgtgcagcg gcgaaaatgg ctgcaataag taatcctgct acaaatggtg gcatctcagt
     8401 caaaatgaaa tatggcacta cagatgatgt attgaagcct tttggtaaaa cagcttcatg
     8461 tgtataaaat gaatacagca ttgtacccat accataaaat aagggtgctg aaattaaagc
     8521 taggatacca tttgtccata acgatttatt tgtttctttt aaactatcag aagcttgata
     8581 acgctgcacg acgtcttgac tcgctgtgta ttgatacaag ttgttgaaaa tatttcctag
     8641 gaaaataatt ggaatggcag ctgccgcagt atttagtttc caattgtctg cactaattaa
     8701 ttttttgtgc tcaatcgcat ctgcaaagac agtgccaaaa ccgcctttaa tgttcataac
     8761 acctagaata ataataacta aagcgccgcc taataaaatg acgccttgaa tgaaatcact
     8821 ccaaaccaca ccttcgaaac cacctaaaaa tgtatataaa atacatagta aacctacgag
     8881 tgatgcaacg atataagggt tcatgtctga tacagatgtg attgctaatg ttggtaagta
     8941 gataacaatt gcaacacgcc ctaaatggta aacgacaaat aataatgagc caatgacacg
     9001 tatgctaggg ccaaatctag cttctaaata ttcatatgca gatgttacct ttaacttttt
     9061 aaagaaaggg acatagaaat aaataagtaa tggaataatt gcgacgatag caatgttacc
     9121 agcgatatat gaccaatctg ttaaaaatgc tttctctggt gtagacataa atgtaatcgc
     9181 acttaacgta gtagcataaa ttgaaaagcc aactacccaa gatggcaagc gaccacttgc
     9241 ggtaaagaaa ctattggtac tttggctcgc gcgcttggta aaataaacgc caatgaacaa
     9301 catagctagt agataaatga taacggcaac ccagtttagt gtgccaaatc caacttcttt
     9361 catgggcaac atccccttta caatgtattg attctttgat gtctataaat cgtattttgc
     9421 aatgagttga tctaatgttt gtcgatgtgc ttcgttaaaa ggtttgaaag gtcttttcgg
     9481 taatcctgca tcaatgtcac gatgacgtaa tatttctttc aatgttggat aaatccccat
     9541 tgataacact gtttcgataa tgtcgtttga atcatgttgt agttggtaag cttcttgaat
     9601 ttgaccttgt cgtgctaagt cgaagatttt tctagcgcgg cgaccattaa cgttatatgt
     9661 agaaccaatt gcaccatcta cgccagaaat cgtagcttga actaacattt catcaaagcc
     9721 agataagatt aatttgtctg ggaatgcttt tctaatacgt tcgagtagga agaagttcgg
     9781 cgctgtatat ttaacaccaa caattttttc gtgattaaat agctcgctga attgttcaat
     9841 agaaatattc acacctgtta aatctggtat tgcataaata atcatattgt tctgtgttgc
     9901 ttcgataata tcgaaatagt aatctctaat ttcttcaaaa gtaaatggat agtagaatgg
     9961 tgttacggca gaaagtgcat cataaccgag ttctgtggca tattttccga gttcaatggc
    10021 ttcatttaaa tctaacgaac ctacttgagc aatcaatttc actttatccc caactgcctc
    10081 tttggcaacc ttgaaaactt gcttcttctg ctctgtattt aataaaaagt tttcgcctga
    10141 gctaccattt acataaagac cgtctaattc ttcagtttca atggcatttt gagcaatttg
    10201 tttaagtcct tgttcattta cttgaccatt ttcatcaaaa ggaacgagta acgctgcata
    10261 taaacctttt aaatctttgt tcattatgaa gtccctccaa aaatcatttg ataatatagt
    10321 ttacagctat aattgtaaac gctatcataa aatgtaacaa tatctttttg aaaattgtag
    10381 tcatatttat gtataattaa tgaaaatgtt tttcaaaatc aatagaaatg gagtgagtaa
    10441 ggtgtattac atcgcaattg atattggagg cactcaaatt aaatcggcag ttattgataa
    10501 gcaattgaat atgtttgact atcaacaaat atcaacgccg gacaacaaaa gtgagcttat
    10561 tactgacaaa gtatatgaga ttgtaacagg atatatgaag caatatcagt tgatccaacc
    10621 tgtcataggt atttcatcag caggcgttgt tgatgaacaa aaaggcgaaa ttggatacgc
    10681 agggccaacc attccgaatt ataaaggtac taattttaag cgattattaa aatcactgtc
    10741 tccttatgtc aaagtaaaaa atgatgtaaa cgctgcatta ctaggcgaat tgaaattaca
    10801 tcaatatcaa gcagaacgga tcttttgtat gacgcttggt acaggcattg ggggtgcgta
    10861 caagaataat caaggtcata ttgataatgg tgagcttcat aaggcaaatg aagttgggta
    10921 tttattgtat cgtccaactg aaaatacaac gtttgagcaa cgtgctgcaa cgagtgcatt
    10981 gaaaaagcgc atgattgacg gaggatttac gagaagcaca catgtgccag tattgtttga
    11041 agcagctgaa gaaggtgatg atattgcaaa acaaatattg aatgagtggg cagaagatgt
    11101 agcagaaggg attgcccaaa tacaagtcat gtatgatcca gggcttatat taattggtgg
    11161 cggtatatct gaacaaggag acaatctcat taaatatatc gagccgaaag ttgcacacta
    11221 tttaccaaaa gactatgttt atgcaccaat acaaacgact aagagtaaaa atgatgcagc
    11281 attatatggc tgtttgcaat gatagttgaa agaaggagtc attctaaaat agaatttgaa
    11341 accgttacga gagatgagag ctgttgttag ttccacacat cacactctat ctaggaccaa
    11401 tctaaactat atcaaccaac agtgtgccac gggcatatta aattgaagac ggttgaacgt
    11461 atagatctca aaaatgtaaa aaagtatttg gtattgaaat taaaaaagca cctagcaact
    11521 cgttaggaca atcacgatga ttgtctacag ttgcaggtgg atttgaatat actactagtt
    11581 atttgttgtc taggataata gatttagtat gttgataagt ttgactcaga tttgtatttt
    11641 ctaataaatg ataactcacg atatcgatta aaaagagtgt cgcaatttgt gtgttgataa
    11701 attgatggtc ggtattacgc gattgatccg ttgttaaaag tactaaatct gcacaatctg
    11761 taagtttact accttcgaaa tttgtgatgg caacgacata tgcaccatga gatttggcga
    11821 cttccgctgc tgaaattaat tccgaagtat taccactatt tgacatagca ataaacatgt
    11881 ccgaatgaga tagtagggat gccgatattt tcattaaatg tgaatcggta gtaacattac
    11941 cttttagccc catacgaatc atacgataat aaaattcagt cgctgataaa ccagagctac
    12001 ctagtccagc aaagagtata tgtcgacttg attggagttt gtcgataaag gtttggataa
    12061 tgtcgttatc aataaattca ccagtttgtt gaatgatttg ttgatgatat ttatgaattc
    12121 tttgaataat tgggctattt tcaataactg tctctgtcat ttcttgttga atattaaatt
    12181 ttaaatcttg gaaattctca taatctagct tatgactaaa gcgtgtcatc gttgctggtg
    12241 atgtaccaat cgcatgggct aaggagttaa tcgttgaaaa ggcatcgcta taaccatttt
    12301 gtcttatata attgacgatg cgtttatcag tttttgtaaa taaatgttga taacgttgaa
    12361 cacgattctc aaatttcatt gtgtcacccc ttcatcttaa tgattactat tatatatgaa
    12421 aaatattttc aagatagtaa aaagcattga taaaaattat cttaatgata tattgtaaat
    12481 gactttacgt gaaaaaacga cttatggagt gaggaataat gttaccacat ggattaatag
    12541 tatcttgtca ggcactagca gatgaaccat tgcattcatc ttttattatg tcgaaaatgg
    12601 cattagctgc gtatgaaggt ggtgctgttg gtattcgcgc aaatactaag gaagacattt
    12661 tagcaattaa agaaacggta gatttaccag ttattggcat tgtgaaacgt gactatgatc
    12721 actcagatgt tttcattact gcaacgtcaa aagaagttga tgaactgata gaaagccaat
    12781 gtgaagtcat tgcattggat gcaacgttac agcaacgtcc gaaagaaacg ttagacgaat
    12841 tagtatcata tattagaaca catgcaccga acgttgaaat catggctgat atcgcgaccg
    12901 ttgaagaagc taaaaatgcc gcacgacttg gctttgatta tattggcacg acgttacatg
    12961 gctatactag ttatacgcaa ggacaattac tttatcaaaa tgacttccaa tttctaaaag
    13021 atgtactaca aagtgttgat gcaaaagtta ttgcggaagg taatgtcatt acaccggata
    13081 tgtataaacg cgtgatggac ttaggcgttc attgttcagt cgttggtggt gcgataacac
    13141 gaccaaaaga aattacgaaa cgttttgttc aagttatgga agattaaatg ataacgataa
    13201 aaaaacgaga tgaccatcat taattaaagg tacctaatta tcttaggtgg ctgaatgaat
    13261 gtaatgggtt catctcgttt tgtttgttta cgatagtgat tttattttca atttgaatta
    13321 tccaaaaata agtaaagcga cggggatggt gattaatagc gacaatgcca cgcgtaaaaa
    13381 ccaaatgatg atgagtttcc agacaggtat tttaatttca gttgctagta tacatggcac
    13441 taatgctgag aaaaagataa tggctgatac gcttactaca ccgacgacaa atttagtact
    13501 cattgcagct ttagttacta acaaagatgg tagaaacatt tctacaatag aaatcgctga
    13561 cgcttttgca agtacagcct gatcagcaat tgggaaaata taaataaatg gatagaatat
    13621 atagccaagc caatcaatga atggtgtata gttcgctaca atcagtccta aaaaaccaat
    13681 cgataatata gaaggcaaaa taccaacagt catttctaaa ccgtctttaa aattgtccca
    13741 aacgttcttc acgagagatg gtgttaatgc attttgtttc atcgcctctg catatgcagt
    13801 tttcagtctg cttccttcaa tagcaacttc ttgttctcct tcttgtccgt tataatattc
    13861 tgttgattca ttggtgattg gcggtagcca tgcagtaatt gcagtcacga caaatgtgat
    13921 gactaaagtt atccaaaagt ataaattcca atgcggcatt agccctaaag ttttagcaac
    13981 gataatcata aaagttgctg aaactgttga aaagccagtc gcaataatcg tggcttctcg
    14041 tttgttgtac atcccttgct tatagacacg attagtaatc aataatccta aggaataact
    14101 gccgacaaac gaagctactg catcgacagc ggattttcct ggtgttttaa aaataggtct
    14161 cataataggc tccatataaa caccgacaaa ttctaataag ccatagccta ctaataaaga
    14221 aagcgcaatt gcacctactg gaattaagat acttaatggc atcattaatt tttcaaacaa
    14281 gaacggacca tagttagctt taaatagtat tgatggaccg attttaaata catacattat
    14341 accaatcatt gcacctgcaa ctttaaataa tgtaatgacc aagtttgtga ttgaagtcat
    14401 aaaagtacgt ctcactattg gtaacgctgt accaattaaa atcataatca gtgcaacata
    14461 gggcataagt ggacctatga ttgagcgaat ggctagatga acatgatcga cgaaaatagt
    14521 gttgttacca ttaatcgtaa aaggaataaa gaaacatagt atgcccacta aactatagac
    14581 aaaaaaacgc catgcacttg gttgttgtgc attagaatga tattgattca ttaaagcaac
    14641 ccctttgttt aaatgaatac acaaaactgt atgatgcatc ttccccttaa tgagatgaat
    14701 cattatttta atttagaaaa atctgaaaac ttactataat tgtatagttt gaattatttt
    14761 cataccaata caaattaact
//
LOCUS       DANBWH010000052        14748 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.054, whole genome shotgun sequence.
ACCESSION   DANBWH010000052 DANBWH010000000
VERSION     DANBWH010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14748
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.054"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..1643
                     /locus_tag="PPY92_001079"
     CDS             <1..1643
                     /locus_tag="PPY92_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR04141 family sporadically distributed
                     protein"
                     /protein_id="HDJ2625095.1"
                     /translation="DKELVSDSNELKLISKVYIRKKDKKLLDWQLLIKNVYLDTEEDD
                     NLFSESGHHFDAILFLKEDTTLQNNVYIIPFGQAYHDINNLIDYDFGIDFAERAIKNE
                     DIVNKNVNFFQQNRLKEIVNYRRNSVDYVRPSESYISVQGHPQNPQIFGKTMTCGTSI
                     SLRVPNRKQQFIDKISVIIKEINAIINLPQKISEFPRIVTLKDLNKIEVLDTLLLKKL
                     SNSSTTENISIDISRFLELSNMILLLDDMLDVNIYINSFKNNTLETFDATDPEVDYIT
                     EIGDYLLKYDVNSINDVRIEVIDNLGHSNNMLLKTILHAEVEMEDGKKYLLQNGKWGY
                     FNREFFDLLNDHLNEIEIRYNTLTPTGLVFKEGEEGYIKEIVGRLPEEYLMLHKKFIK
                     PINKNFIVKGNGIELADLYNIKNKELFTIKRGINTSLSLYSLEQNIIAINALKYPESY
                     NFEELIEAIPDNSENIFNDIQRSTNFSIVWILPISSIDNMPIKDMVHTSNVINKNFQL
                     TNLGSVLLKNKLVEWSLYLKDQRINPIIYMETPTEDRN"
     gene            complement(1741..2096)
                     /locus_tag="PPY92_001080"
                     /pseudo
     CDS             complement(1741..2096)
                     /locus_tag="PPY92_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
     gene            complement(2401..2489)
                     /locus_tag="PPY92_001081"
                     /pseudo
     CDS             complement(2401..2489)
                     /locus_tag="PPY92_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498638.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2587..3342)
                     /locus_tag="PPY92_001082"
     CDS             complement(2587..3342)
                     /locus_tag="PPY92_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDJ2625096.1"
                     /translation="MDIVNIVIMLLIGVFGGFISGLVGVGGAIIIYPAILLLPPLFGA
                     PAYSAYIASGLTSSQVFFSTLSGSLKARKKTEFSPQLVLYMGGGMIIGSMLGAFLANL
                     FDATFVNTVYIIIALLALTLMFIKVKPSSEKSSFNKYLLVIIGLFIGIISGIVGAGGA
                     FIIIPILLVLFKLPMNTVVANSIVIAFISSIGAFVIKLIQGYIPLYDALFLIIGSIIF
                     APIGLKLSKKVPNIIQKWIISILIVFAIVQLIL"
     gene            complement(3342..3602)
                     /gene="cstR"
                     /locus_tag="PPY92_001083"
     CDS             complement(3342..3602)
                     /gene="cstR"
                     /locus_tag="PPY92_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide-sensing transcriptional repressor
                     CstR"
                     /protein_id="HDJ2625097.1"
                     /translation="MNYDKKMINRINRIQGQLNGIIKMMEEGKDCKDVITQISASKSS
                     LQRLMGIIISENLIECVKVAADDEESSQELINEAVNLLVKSK"
     gene            3739..4803
                     /gene="cstA"
                     /locus_tag="PPY92_001084"
     CDS             3739..4803
                     /gene="cstA"
                     /locus_tag="PPY92_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide response sulfurtransferase CstA"
                     /protein_id="HDJ2625098.1"
                     /translation="MKQYGEKFIDEFSKAELEKLAKQGQLIDVRTEEEYALGHINGSI
                     LHPVDEIESFNKEKNKTYYVICRSGNRSANASKYLAKQGYNVINLDGGYKAYEENDSY
                     DTQEEYKSIEIKADRKQFNYRGFQCPGPIVKISQEMKNIEVGDQIEVKVTDPGFPSDI
                     KSWVKQTRHTLVKLDENNNGINAIIQKEKAKDLDINYSAKGTTIVLFSGELDKAVAAL
                     IIANGARAAGKDVTIFFTFWGLNALKKVQTVNVKKQGIAKMFDLMLPQKNIRMPLSKM
                     NMFGLGNMMMRYVMKKKNVDSLPTLINQAIEQNIKLIACTMSMDVMGIQKEELRDEVE
                     YGGVGTYIGATENANHNLFI"
     gene            4834..6168
                     /gene="cstB"
                     /locus_tag="PPY92_001085"
     CDS             4834..6168
                     /gene="cstB"
                     /locus_tag="PPY92_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide dioxygenase-sulfurtransferase CstB"
                     /protein_id="HDJ2625099.1"
                     /translation="MFFKQFYDNHLSQASYLVGCQRTGEAIIIDPVRDLSKYIEVADS
                     EGLTITQATETHIHADFASGIRDVAKRLNANIYVSGEGEDALGYKNMPSKTQFVKHGD
                     IIQVGNVKLEVLHTPGHTPESISFLLTDLGGGSSVPMGLFSGDFIFVGDIGRPDLLEK
                     SVQIKGSTEISAKQMYESVQNIKNLPDYVQIWPGHGAGSPCGKALGAIPISTIGYEKI
                     NNWAFNEIDETKFIESLTSNQPAPPHHFAQMKQVNQFGMNLYQSYDVYPSLDNKRVAF
                     DLRSKEAFHGGHTKGTINIPYNKNFINQIGWYLDFEKDIDLIGDKSTVEKAKHTLQLI
                     GFDKVAGYRLPKSGISTQSVHSADMTGKEEHVLDVRNDEEWNNGHLDQAVNIPHGKLL
                     NENIPFNKEDKIYVHCQSGVRSSIAVGILESKGFENVVNIREGYQDFPESLK"
     gene            6187..7380
                     /gene="sqr"
                     /locus_tag="PPY92_001086"
     CDS             6187..7380
                     /gene="sqr"
                     /locus_tag="PPY92_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II sulfide:quinone oxidoreductase Sqr"
                     /protein_id="HDJ2625100.1"
                     /translation="MNKHYQIVIIGGGTAGVTVASRLLRKNQNLKEKIAIIDPADHHY
                     YQPLWTLVGAGVSSLKSSRKDMESVIPEGANWIKQAVSSFQPENNSVILGDNTVVYYD
                     FLVVAPGLQINWSSIKGLKENIGKNGVCSNYSPDYVNETWNQISNFKQGNAIFTHPNT
                     PIKCGGAPMKIMYLAEDYFRKHKIRSNANVIYATPKDALFDVGKYNKELERIVEERNI
                     TVNYNYNLVEIDGDKKVATFEHIKAYDRKTISYDMLHVTPPMGPLDVVKESTLSDSEG
                     WVDVNPTTLQHKSYSNVFALGDASNVPTSKTGAAIRKQAPIVANNLLQVMNNQMLTHH
                     YDGYTSCPIVTGYNRLILAEFDYNKNTKETMPFNQAKERRSMYIFKKDLLPKMYWYGM
                     LKGLI"
     gene            complement(8046..9032)
                     /locus_tag="PPY92_001087"
     CDS             complement(8046..9032)
                     /locus_tag="PPY92_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDJ2625101.1"
                     /translation="MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEF
                     TNTESFCHPEGIHSVRGRLTFSEDEHPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLN
                     MGCPVANVAKKGKGSGLILRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKH
                     VFEQDIANLSIHLRTRKEMSKVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGL
                     ELAEKYGIDGVMIGRGIFHNPFAFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQF
                     KSLRRFFKIYVRGIRGASELRHQLMNTQSIAEARALLDEFEAQMDEDVKIEL"
     gene            9341..9544
                     /locus_tag="PPY92_001088"
     CDS             9341..9544
                     /locus_tag="PPY92_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625102.1"
                     /translation="MEDLKESLKSLGWWDLFFAIPMFLLFAYLPNYNFITIFLNIVII
                     IFFSIGLILTTHIIIDKIKSNTK"
     gene            9593..9889
                     /locus_tag="PPY92_001089"
     CDS             9593..9889
                     /locus_tag="PPY92_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HDJ2625103.1"
                     /translation="MATKKDVHDLFLNHVNSNAVKTRKMMGEYIIYYDSVVIGGLYDN
                     RLLVKATKSVRHQLQDNTLVSPYPGAKEMILIPDITEAINLTNLFELIKNDLKN"
     gene            10097..10699
                     /locus_tag="PPY92_001090"
     CDS             10097..10699
                     /locus_tag="PPY92_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625104.1"
                     /translation="MLISLVIPVFALLVIGGIIWVIIEGIVHISKKNKAIDNFLNQVN
                     KVSETYKFATAFLFLILATAGISQFYLYYIVSAFLFWLVILTFGIAGIIFLMPYGLCF
                     LPLYKQKKKKQTFKKYMVYTTIGLSICLGLSLVLVHTTKIYMDEGGVRYYYGSFVMKQ
                     AGGYAYLALAVLSTLLIVAKKATNKNKEIETVDNTNITER"
     gene            10966..14118
                     /locus_tag="PPY92_001091"
     CDS             10966..14118
                     /locus_tag="PPY92_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA2/NAM7 family helicase"
                     /protein_id="HDJ2625105.1"
                     /translation="MSQLLNDTLSAWLLIESLSPGEVNFTAEDILSAEHFKNGAKQAQ
                     LQSFDEYFEIWNSERFIISEEKSETGELIFKFYRHCFRYNEINLKIQDIFDDYSDIHN
                     PNGTHCYGYTFNTDKHGKVIVDSIHIPMIMSALKEIEKNKNANIEEKFNDSVEKFFQK
                     VKEILADEPINEFKLKKMDKAYDEYFSVLNSKKDGLFGHYVAIEYVKDSDLPQPEFNS
                     FFISDIEKARKSPNQTLIDYIEGVEESQRIEVDENKEMFDKFLHPSRLPDGRWPSQTE
                     FRLSLMQQLAVNQITSGNERISSVNGPPGTGKTTLLKDIFAHLVVERGKELAKLNNPK
                     DAFVKTKIHETDDKYVYLLKESIAKYKMVVASSNNGAVENISKDLPKIEEIIRNPEKC
                     KFPKYEQNYANLAHELKDFAEIAEDLIGESAWGLFSGVFGKSTNINQVLSHMLKQDAN
                     DIGFAKLLQNENNRMSYNELMSEWQSHQRAFLEELRHVEMLKEESIRAYDVYKNCESF
                     SKIEQVINSEKTSIEEQVYHLDNETLRDNKEIEDLDNRINYIVKQIETLNELIKSIKE
                     SNKGFINKLKAMFNSEEDESYKDHNKEKQQLLTQQLELEKCKKNKHEDLVSKLKEKEK
                     LIKQLTKVQLQLDELNSQLQELEAYRIESKITIPEKDFWSDNNYDERQVTNLWTSDEL
                     QYRRAMLFLRAMILHKLLLIANNTTIYYAINDFKDRRKLIDANPDKVHNAWNVMHLIF
                     PVVSTTFASFKSMYGGIPKDFIDYLFIDEAGQAIPQAAVGALYRSKKVVAVGDPIQIE
                     PVVTLESHLIDNIRKNYHVPEYLVSKEASVQSVADNANQYGFWKSDATDSNQKTWIGI
                     PLWVHRRCLKPMFTIANQIAYNNKMVLPSNITKVGKTGWYDVKGNAVQKQFVKEHGEK
                     VVGLLADDWIEAIKEGKNEPSSFVISPFSAVQQQIKRMLKQQLPTRIDIERTKINQWV
                     DKSIGTVHTFQGKEAQKVYFVIGTDNTQDGAVNWSCEKPNLLNVAVTRAKKEFYVIGD
                     MQRIQMKPFYETIFKERNVK"
     gene            14175..14657
                     /locus_tag="PPY92_001092"
     CDS             14175..14657
                     /locus_tag="PPY92_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625106.1"
                     /translation="MEKFNNWILNAISGSQTDKNETTEELKGAKFIILYAYSMLVLLA
                     LVISNIFIHILEPKLSITTQIIIVLILIEALIGLRFLKAYDVKRGKDKENKKNSKDFV
                     KLKSILVAILFTSLALTAGTVADIYGFTDLGNTRSDLIVWSIGGIIFGLVCYTMEDKK
                     "
BASE COUNT         5436 a         2059 c         2523 g         4730 t
ORIGIN      
        1 atgataaaga acttgttagt gattcaaatg aattaaaatt aattagtaaa gtttatatac
       61 gtaaaaaaga caaaaaacta ttagattggc aattattaat aaagaatgta tacctagata
      121 ctgaagaaga tgacaattta ttttcagaat ccggtcatca ttttgatgca atattatttc
      181 tcaaagaaga tactacatta caaaataatg tatatattat accttttgga caagcatatc
      241 atgatataaa taatttgatt gattatgact tcggaattga ttttgcagaa agagcaatca
      301 aaaatgaaga catagttaat aaaaatgtta atttttttca acaaaacagg cttaaagaga
      361 ttgttaatta tagaaggaat agtgtagatt acgttagacc ttcagaatct tatatatcag
      421 tccaaggaca tccacagaat cctcaaattt ttggaaaaac gatgacttgt ggtacaagta
      481 tttcattgcg tgtaccgaat agaaagcagc aattcataga taaaattagt gtgataatca
      541 aagaaataaa cgctattatt aatcttcctc aaaaaattag tgaatttcct agaatagtaa
      601 ctttaaaaga cttgaataaa atagaagtat tagatacttt attgctaaaa aaactatcga
      661 attcttcaac tacagaaaat atatctatag atatatcaag atttttagaa ctaagtaata
      721 tgatactctt gttagatgat atgctcgacg tcaatatata tataaattct tttaaaaata
      781 atacattaga gacatttgat gcgactgatc ctgaagttga ttacatcact gaaataggag
      841 attacttatt aaaatatgac gttaattcta taaatgatgt cagaattgaa gtgatagata
      901 atttggggca ttcaaataat atgctactaa aaacgatact acatgcagaa gttgaaatgg
      961 aagatggaaa gaaatattta ttacaaaatg ggaaatgggg ttattttaat agagaatttt
     1021 ttgacctttt gaatgatcat ttgaatgaga tagaaattag gtataacaca ctaactccca
     1081 caggtttagt atttaaagaa ggagaagaag gatatataaa agaaatagta ggaagattgc
     1141 cagaagaata tttaatgtta cataaaaaat tcataaaacc aataaataag aattttatag
     1201 taaaaggaaa tggaatagag ttggcagact tatacaatat taaaaacaaa gagcttttca
     1261 cgattaaaag aggaattaat acatctttat ctctttatag tctagaacag aatataatag
     1321 caattaacgc cttaaaatat ccagaatcat ataattttga agaattaata gaagctattc
     1381 ctgataactc ggaaaatata tttaatgata tacaacggag tacaaatttt agtatagtat
     1441 ggattttacc gatatcatct attgataata tgcccataaa agatatggtt catactagta
     1501 acgtaataaa taaaaacttt caattaacta atttaggttc agtattactt aaaaataaat
     1561 tagtagagtg gtctctctat ttaaaagatc aaagaattaa tccaattatt tatatggaaa
     1621 cgccaactga agatagaaat taaactttta ttttaaatca ccctagttca tacctaaaag
     1681 actttcacac aaacaaacaa aggaggaaac ttaaattcct cctttccctt attactcata
     1741 ctataattca attttaacgt cttcgtccat ttgggcttca aattcatcta gtaatgctcg
     1801 tgcttctgca attgattgtg tgttcatcaa ttgatgtcga agttcgctag cgctcttatg
     1861 ccacgcacat agattttaaa gaatctacgc aaactcttga attgtcgtat ttcatctttt
     1921 tcatatttgt taaacaatga taaatgcaat ctcaacagat ctaatagttc cttgcttgtg
     1981 tgttcgcgtg gttctttttc aaaagcgaat ggattatgga aaatgccgct accaatcatg
     2041 acgccatcaa tgccatattt ttctgcaagt tcaagtcctg tttttctatc gggaatatgg
     2101 gagtgggaca gaaatgatat tttcccaaaa tttatttcgt cgtcccactc caacttgcac
     2161 attattgtaa gctgactttc cgccagctac tatgttgggg cccctgacta gaattgaaaa
     2221 aacttgttac aagtgcattt tcgttcagtc aactactgcc aatataactt tgtagagcat
     2281 agaacattga tttatgtccc aacctgcaat ttcgtcacgt aaatttttaa tagcttcgat
     2341 taattcccac tgtgcatcta ctttactaat gtgtttgata aaaacttaaa taattttaat
     2401 tcagtcatca gtggcattaa atcttctatc atttttagtt atagttgata aatttatatt
     2461 tataagcata tatatggata tttcatcaag aatttttaat catataaatc cgaactgcat
     2521 acatatttgt tgaaataaga ggtattattt ttcgggaaat tgctgtctga gttataagta
     2581 ttagttttat aaaatgagtt gaactatagc aaaaacgatt aaaatactga taatccattt
     2641 ttgtattatg ttagggactt ttttacttaa ttttaaccct attggagcaa atataatact
     2701 ccctattata aggaataagg cgtcatataa agggatataa ccttgaataa gtttgatgac
     2761 aaaagcacca attgaagata taaaagcaat tactatacta ttagcgacta cagtattcat
     2821 tggtaatttg aataaaacca ataatatagg aataataatg aaggcaccac ctgcacctac
     2881 tatacctgaa ataataccaa tgaaaaggcc aatgataact aataaatatt tattaaatga
     2941 agacttttcg gaactaggtt tcactttaat aaacattaat gttaatgcaa gtaaagcaat
     3001 aatgatatat accgtattta caaatgtagc atcaaataaa tttgctagaa atgcacctaa
     3061 catactccct ataatcatgc cgccacccat ataaagaact aattgtggtg agaactctgt
     3121 tttttttcga gcttttaatg agccacttaa tgtactgaaa aagacttggc tagaagtaag
     3181 acctgatgcg atatatgcgc tatatgcagg ggctccgaat aatggtggta ataataaaat
     3241 agctggataa ataatgatag caccacctac gcctactaga ccagatatga acccaccgaa
     3301 taccccaatg agtaacatga taactatatt aacaatatcc attacttact tttcaccaat
     3361 aagtttacag cttcattaat taactcttgg gagctttctt catcatccgc agctactttt
     3421 acacattcta ttaaattctc actaataatg atacccatca agcgttggag tgaactcttt
     3481 gatgcactta tttgtgtaat gacatcttta cagtcttttc cttcctccat cattttaata
     3541 attccattta gttgcccttg tattctatta atacgattaa tcattttttt atcataattc
     3601 atagtcatac ctccactttt aattgaataa aaatatatta atagataaac acaaatgtgt
     3661 caaatacccc tagaggtatt tgacaagttc cattcaaccg tttagaatac ccctacaggt
     3721 atttttaggg aggttattat gaaacaatac ggagaaaagt ttatcgatga atttagtaaa
     3781 gcagaattgg aaaaactagc caagcaaggg caattaattg atgttagaac agaagaggag
     3841 tatgcattag gacatatcaa tggttccata cttcatcctg ttgatgagat tgagtcattc
     3901 aataaagaaa aaaataaaac ctattatgta atctgtagaa gtggtaacag aagtgctaat
     3961 gctagtaaat atttagctaa acaaggttat aacgttataa atcttgatgg tggttataaa
     4021 gcttatgaag aaaacgatag ttatgataca caagaagaat ataaaagtat agaaattaaa
     4081 gcagatcgta aacaatttaa ctatcgtggt tttcaatgtc cagggccaat tgtaaaaatt
     4141 agtcaagaaa tgaagaatat tgaagtaggt gaccaaattg aagtcaaagt cacagaccct
     4201 ggattcccta gtgatattaa aagttgggtg aaacaaacaa ggcatacttt agttaagctt
     4261 gatgaaaata acaatggaat taatgcgatt attcaaaaag aaaaagcaaa agatttagat
     4321 ataaattatt ctgctaaagg tactacaatt gtattattta gtggagaatt agacaaagct
     4381 gtagcagcat tgattattgc aaatggcgct agagctgctg gaaaagatgt aactatcttc
     4441 tttacttttt gggggcttaa tgcattaaaa aaagtgcaaa cagttaatgt taaaaagcaa
     4501 ggtattgcaa aaatgtttga tttaatgttg ccccaaaaga atatacgaat gcctctttcc
     4561 aaaatgaata tgtttggttt aggaaatatg atgatgcgct acgtaatgaa aaagaaaaat
     4621 gttgattcat taccaacact tatcaatcaa gctattgagc aaaatatcaa attaatcgct
     4681 tgtacgatga gtatggatgt catgggtatt cagaaagaag aacttagaga tgaagttgag
     4741 tacggtggtg taggcactta tattggtgct actgaaaatg cgaatcataa tttatttatc
     4801 taattaaatc tattaataaa aggagttgtt atcatgtttt ttaaacagtt ttacgataat
     4861 catttatctc aagcatcata tttagtgggt tgtcaacgta caggagaggc aataataata
     4921 gaccctgttc gtgatttatc gaaatatata gaagttgcag attctgaagg tttaacaatt
     4981 acacaagcta cagaaacaca tattcatgct gattttgctt caggaattcg tgatgtggct
     5041 aaacgcttaa atgcaaatat atatgtgtct ggcgaaggtg aagatgcatt agggtataaa
     5101 aatatgccat caaaaacaca atttgttaaa catggagata tcattcaagt aggcaatgtt
     5161 aaattagaag ttctgcatac tccaggacac acgcctgaaa gtattagctt tttactcact
     5221 gatttaggtg gtggttcaag tgttccgatg ggattattta gtggtgactt tatttttgtt
     5281 ggtgatatag gtagacctga tttattagaa aaatctgttc aaataaaggg ttctacagaa
     5341 attagcgcga aacaaatgta tgagtccgtt caaaatatta aaaatttacc agactatgtt
     5401 caaatctggc cgggtcatgg tgctggaagc ccttgtggta aagcattagg tgccatacct
     5461 atatctacaa taggttatga gaaaattaat aactgggcat ttaatgaaat tgatgagact
     5521 aaatttattg aatcattaac atcaaatcaa ccagcaccac cgcatcattt tgcacaaatg
     5581 aaacaagtta atcagtttgg tatgaattta tatcaatcat atgatgttta tcctagttta
     5641 gataataaga gagtagcatt tgatcttcgt agcaaagagg cctttcacgg tggccacaca
     5701 aaaggaacaa tcaatatacc atacaacaaa aactttatta atcaaattgg ttggtactta
     5761 gattttgaaa aagatataga tttaattgga gataaatcta ctgttgagaa agcgaaacac
     5821 actttacaat taattgggtt tgataaggta gcaggctatc gtttgccaaa atcaggcatt
     5881 tcaacccagt ccgttcatag cgctgatatg acaggtaaag aagaacatgt attagacgta
     5941 cgtaatgatg aagagtggaa taatggacac ttagatcaag cagttaatat tccacatggt
     6001 aaattattaa atgaaaatat tccttttaat aaagaggata aaatatatgt acattgtcag
     6061 tcaggtgtta gaagttcaat tgcagtgggt atattggaaa gcaaaggttt tgaaaatgtg
     6121 gtgaatatta gagaaggcta tcaagatttt ccagaatcat taaaataatt taaggatgtg
     6181 gaaaaaatga ataagcatta tcaaattgtt attattggtg gcggtacagc aggtgttacc
     6241 gtagcatcaa gactattaag aaaaaatcaa aacttaaaag agaaaatagc aattatagac
     6301 ccagcagacc atcattacta tcaaccatta tggacgttgg ttggtgcagg ggtatctagt
     6361 ttgaaaagtt ctcgtaaaga tatggaaagt gttatacctg aaggtgctaa ctggataaaa
     6421 caggctgttt caagttttca acctgaaaat aatagcgtta ttttaggaga taatacagtc
     6481 gtttattatg attttttagt agtagctcca ggattacaga ttaattggtc ttcaattaaa
     6541 ggactaaaag aaaatatagg taaaaatggt gtttgctcta actattcacc tgactatgtt
     6601 aacgaaactt ggaaccaaat ttctaatttt aaacaaggaa atgccatttt tacgcatcca
     6661 aacactccta taaagtgtgg aggtgcgcct atgaaaatta tgtatttagc tgaagattat
     6721 tttaggaaac ataaaatccg ttctaacgct aatgtgatat atgcaacgcc aaaagatgct
     6781 ttatttgacg taggaaaata taataaagaa ttagaaagga ttgttgaaga aagaaatata
     6841 acagtcaatt ataattataa ccttgttgaa atcgacggtg acaaaaaagt ggctacattc
     6901 gaacatatca aagcatacga tagaaaaaca ataagttatg atatgttaca tgtaacacca
     6961 cctatgggtc ccttagatgt agtaaaagaa agtacacttt cagatagtga gggttgggta
     7021 gatgttaacc caaccacatt acagcataaa agctactcta atgtatttgc acttggtgat
     7081 gcttcaaatg tacctacttc aaaaacaggc gcagctattc gtaagcaagc acctatcgtc
     7141 gctaataatt tattgcaagt gatgaataat caaatgttaa cgcatcatta tgatggttat
     7201 acttcatgcc ctattgttac tggatataat aggttaatac ttgcagagtt tgattataat
     7261 aaaaatacta aagaaacaat gccgtttaat caggccaaag aacgtagaag tatgtatata
     7321 tttaagaaag atttattacc taaaatgtat tggtacggca tgctaaaagg attaatataa
     7381 taaagtacag aaaacaataa atttttaatg aaaaatcttt tactataaaa gattaagtat
     7441 ttaaatgacg tgtcagtgtt gtgtttatat gtcgtgaatt tttagctcta aatagtataa
     7501 gattgaaaaa gttgttactg ttttaaatga tcacgatgaa gtcattcaat aagaatgatt
     7561 atgaaaatag aaacagcagt aagatatttt ctaattgaaa atcatctcac tgctgttttt
     7621 taaaggttta tacctcatcc tctaaattat ttaaaaataa ttaatggtat ttgagcacgt
     7681 ttagcgactt tatgactgac attaccaatt tccatttctt gccagatatt caaaccacgt
     7741 gtactcaaaa tgatagcttg gtatgtacct ccaatagtaa tttcaataac tttgtctgtt
     7801 gaacactaag agcaatttta atttcataat gtgttgtaaa catttttttg attggagttt
     7861 ttttctgagt taaacgatat cctgatgtat ttttaatttt gcaccatttc caaaaggata
     7921 agtgacataa gtaaaaaggc atcatcggga gttatcctat caggaaaacc aagataatac
     7981 ctaagtagaa agtgttcaat ccgtgttaaa ttgggaaata tcatccataa actttattac
     8041 tcatactata attctatttt aacgtcttcg tccatttggg cttcaaattc atctagaagt
     8101 gctcgtgctt ctgcaattga ttgtgtgttc atcaattgat ggcgaagttc gctagcgcct
     8161 cttatgccac gcacatagat tttaaagaat ctacgcaagc tcttgaattg tcgtatttca
     8221 tctttttcat atttgttaaa caatgataaa tgcaatctca atagatctaa tagttccttg
     8281 cttgtgtgtt cgcgtggttc tttttcaaaa gcgaatggat tatggaaaat gcctctacca
     8341 atcatgacac catcgatgcc atatttttct gccagttcaa gtcctgtttt tctatcggga
     8401 atatcaccgt taattgttaa caatgtattt ggtgcaattt cgtcacgtaa atttttaata
     8461 gcttcgatta attcccaatg tgcatctact ttactcattt ctttacgtgt acgaagatga
     8521 atagataaat tggcaatatc ttgttcgaag acgtgcttca accaatcttt ccattcatcg
     8581 atttcatagt agccaaggcg tgttttaaca cttaccggaa gcccacctgc tttagttgct
     8641 tgaataattt cggcagcgac atccggtctt aagattaagc cggaaccctt acccttttta
     8701 gcaacatttg ctacaggaca tcccatattt aagtcaatgc ctttaaagcc cattttagct
     8761 agttgaatac tcgtttcacg gaactgttct ggcttatctc cccatatatg agcgaccatc
     8821 gggtgttcat cttcgctaaa agttaagcgt ccgcgcacac tatgtatgcc ttcagggtgg
     8881 caaaagcttt cagtatttgt aaattcagtg aaaaacacat ccggtctagc tgcttcactt
     8941 acaacgtgtc gaaagacgat atctgtaacg tcttccattg gcgccaaaat aaaaaatgga
     9001 cgtggtaatt cactccaaaa attttctttc ataatatatt tataccctct ttataattag
     9061 tatctcgatt ttttatgcat gatgatatta ccacaaaagc ctaacttata caaaaggaat
     9121 ttcaatagtt gcaaccattt gaaagggaag tctaagagta gtctaaaata aatgttgtgg
     9181 taagttgatc aatacaaaga tcaaggatta tagtattaaa ttgttcatta ttaatgatac
     9241 actacttatg aatatgattc agaattttct ttggctactt ttacagtaaa gcgatttttt
     9301 agttatctta taacaaagac aaatttataa aggtgatatt atggaagatt taaaggaatc
     9361 tttaaaaagt ttaggttggt gggatttatt ttttgcgata cctatgtttc tgctattcgc
     9421 ataccttcca aactataatt ttataacgat atttcttaac attgttatca ttattttctt
     9481 ttccataggt ttgattttaa ctacgcatat aattatagat aaaattaaga gcaacacgaa
     9541 atgaatcatt aatacgaatg tgattaaaca taaaactgaa ggagcgatta caatggcgac
     9601 taagaaagat gtacatgatt tatttttaaa tcatgtgaat tcaaacgcgg ttaagacaag
     9661 aaagatgatg ggagaatata ttatttatta tgatagtgtg gttataggtg gtttgtatga
     9721 taatagatta ttggttaagg cgactaaaag tgtacgtcat caattgcaag ataatacatt
     9781 agtttcgcca tatccaggtg ccaaagaaat gatattaatt ccagacatta ccgaagcaat
     9841 aaatctcact aatttatttg agctcataaa aaatgatttg aaaaactaat cataaattgc
     9901 catactcaac tattttgaaa agtatcaatt tcatattatc aaattattga gtatctcgat
     9961 ttattgttat acacaaacag tatttttgct atgatagagt taataaatat aataaattga
    10021 ataagtaact ttacaaaact agaatgattc aattcaatca caattatttg aaaatagagg
    10081 tgagcaggtg aacgatatgt taattagtct cgtaatccca gtttttgctt tgttagttat
    10141 tggtggtatt atttgggtga ttatagaagg tatagtacat atttcaaaaa agaataaagc
    10201 aatcgataac tttttaaacc aagttaataa agtaagtgag acatataaat tcgctactgc
    10261 ttttttattt ctaatcttgg ctacggctgg tatttctcaa ttttatctat attatatagt
    10321 atcagcgttt cttttttggc ttgtcatact tacctttggt attgcaggta tcattttttt
    10381 aatgccatat ggattatgtt tcctaccact ttataagcaa aaaaagaaaa aacagacatt
    10441 taaaaaatac atggtttaca ctacgattgg tttgtcaatt tgtctaggct tatctctagt
    10501 tttggttcac actacgaaaa tttatatgga cgaaggtggc gtaagatact attacggtag
    10561 ttttgtaatg aaacaagcgg gcggttatgc ttatttagct ttagcggtac tttcaacgtt
    10621 gttaattgtt gcgaaaaaag ctacaaataa aaataaagaa atcgaaaccg tcgacaatac
    10681 aaatataacg gaaagataat taagggagtg ctcattcagg agtgctcttt tttgatgtcc
    10741 aaatttagtt gcaaatgaag gcatagaaga aatttttata gtcatttatt ggataataaa
    10801 ttatggtcat atcgattaat tttattagtg aggattttac aaacatttgt tgaataaata
    10861 tggtaatgat aatatatgaa tatttttgaa aaaacactaa tatgattaaa ttatttgtta
    10921 aaatagcatt aagtaaaaaa caaataggga acaaggtggg atttcatgag tcaattgcta
    10981 aatgatacgt tatcggcttg gttgttaatt gaatctttaa gtccaggaga agtaaatttt
    11041 acagcggaag atatactctc agctgaacat tttaaaaatg gtgcaaagca agcacaactt
    11101 caaagttttg atgaatattt tgaaatatgg aattctgaac gctttattat atcagaagaa
    11161 aaatcagaga ctggggaact tatatttaaa ttttatagac attgcttccg ctataatgaa
    11221 attaatttga aaattcagga tatttttgat gattattctg atattcataa tccaaatggg
    11281 acacactgtt atggttacac atttaacaca gataaacacg gcaaagtgat agttgattct
    11341 atacatattc cgatgattat gagtgcatta aaagaaattg aaaagaacaa aaatgccaat
    11401 atagaagaaa aatttaatga ttctgttgaa aaattttttc aaaaagtaaa agaaatttta
    11461 gcagatgaac caattaatga atttaaattg aagaagatgg acaaagctta tgatgagtac
    11521 ttttctgtat taaattcaaa gaaagatgga ttatttggac attatgtagc aatagaatat
    11581 gtgaaagata gtgatttacc acagccggaa tttaacagtt tcttcataag tgatattgag
    11641 aaagcaagaa aatctcccaa ccaaacttta attgattaca ttgaaggtgt agaagaaagt
    11701 cagcgcatag aagtagatga aaataaagaa atgtttgaca aatttttaca tccttcacgt
    11761 ttgcctgatg gacgatggcc atcacagact gagtttagat tgtctttaat gcaacaactt
    11821 gctgtaaacc aaattacgag tggtaatgaa agaataagtt cagttaatgg gccaccaggg
    11881 acaggtaaga ctactttatt aaaagatata tttgctcatc tagtagttga aagaggtaaa
    11941 gagttagcta aactaaataa tcctaaagat gcatttgtca aaacaaaaat tcatgaaacg
    12001 gatgataaat acgtatactt actaaaggaa tctattgcca aatataagat ggtagtcgca
    12061 tctagtaata atggagctgt tgaaaatata tctaaagatt taccgaaaat tgaagaaatt
    12121 ataagaaatc ccgaaaaatg taaattccct aaatatgaac agaattatgc aaatttagca
    12181 catgaattaa aagattttgc tgaaatagct gaagatttga ttggtgaaag tgcctggggc
    12241 ttattttctg gagtttttgg taaaagtact aatattaacc aagtattgag tcatatgtta
    12301 aaacaagatg cgaatgatat tggctttgct aaattactac aaaatgagaa taatcgtatg
    12361 agttataacg agttaatgag tgaatggcaa tcacatcaac gtgcattttt agaagagttg
    12421 aggcatgttg aaatgttaaa agaagaatct attagagcat atgatgttta taaaaattgt
    12481 gagtctttct ctaagattga acaggttatt aatagtgaaa aaacaagtat tgaagaacag
    12541 gtatatcatt tagataatga aacgttacga gacaataaag aaatagaaga tttggataat
    12601 cgaattaatt atattgttaa gcaaatagaa actttaaatg agttaattaa atccatcaaa
    12661 gaaagcaaca aaggttttat taacaaactg aaagcgatgt ttaattcaga agaagatgaa
    12721 agctataaag atcataataa agagaagcaa caattattaa cacaacagtt agagttagag
    12781 aaatgtaaaa aaaacaaaca tgaagacctt gttagcaaac taaaagaaaa agagaaatta
    12841 attaaacaat taactaaagt acagttgcaa ttagacgagt taaattcaca gttacaagag
    12901 ttagaagcat atcgtattga gtcaaaaatt acaattccag aaaaagattt ttggagtgac
    12961 aacaattatg atgagcgcca agttactaat ctgtggacga gtgacgaact tcaatacaga
    13021 cgtgccatgc tctttttaag agcaatgata ttgcataaat tattattgat tgctaataat
    13081 acaactattt attatgcgat taatgatttt aaagatagaa ggaaattaat tgatgcaaat
    13141 ccagataaag tacacaacgc atggaatgtg atgcatttaa tatttccagt agttagtacg
    13201 acgtttgcaa gctttaaatc tatgtatggg ggcataccaa aagatttcat agactactta
    13261 tttattgatg aagcaggaca agcaatacct caagcagctg tgggagcatt atatcgttca
    13321 aaaaaagttg tagctgtagg tgatccgatt caaatagaac cggttgtgac tttagaaagt
    13381 catttaattg ataacattcg taaaaattat catgttccgg aatatctagt ttctaaagaa
    13441 gcttctgtgc agtctgttgc agacaacgcc aatcaatatg gtttttggaa atctgatgct
    13501 actgatagta atcaaaaaac ctggataggc atacctttat gggtgcacag acgatgttta
    13561 aaacctatgt tcacgatagc taaccaaatc gcttataata ataaaatggt gttgccaagt
    13621 aatattacaa aagtaggtaa aacaggttgg tatgacgtta aaggaaacgc agttcaaaaa
    13681 caatttgtga aagagcatgg tgaaaaagta gtgggattat tagctgatga ttggattgaa
    13741 gcaattaagg aaggtaaaaa tgaaccgagc tcatttgtaa tatcgccttt ttcagcagta
    13801 cagcaacaga ttaaacgtat gttaaagcaa caactaccga ctagaattga tattgaacgt
    13861 acaaaaatta atcaatgggt cgataaatcc attggtactg ttcatacttt tcaaggtaaa
    13921 gaggctcaga aggtgtattt tgtaataggt actgataata cccaagatgg tgctgtgaac
    13981 tggtcatgcg aaaaaccaaa cttgttaaac gttgcagtga caagagctaa gaaagagttt
    14041 tatgtaattg gcgacatgca aagaatacag atgaaaccat tttatgagac gatttttaaa
    14101 gaaagaaatg taaaataaca tacaaaaaag tatatagagg aagttataca ttttaaaagg
    14161 agcaaaattg aataatggag aaatttaaca actggatatt aaatgcaata agtggatctc
    14221 aaacagacaa gaatgaaaca actgaagaat taaaaggggc aaaatttatc attttatatg
    14281 catattcaat gctcgttttg cttgcgttag taatttctaa catattcatt cacattttgg
    14341 agcctaaact atcaatcacc actcaaatca tcatcgtttt gattttaatt gaagcactaa
    14401 ttggactgcg tttcttgaaa gcgtacgatg ttaagcgtgg caaagataaa gaaaataaga
    14461 aaaatagtaa ggatttcgtt aaactaaaat caattttagt agcaatttta tttacatcat
    14521 tggcgctgac agcaggtact gtagctgata tatacggttt cactgactta ggaaatacta
    14581 gaagtgattt aatcgtttgg agcataggtg gtattatatt tggcctcgta tgttacacaa
    14641 tggaagataa aaaataacga taaggagctg gcgattataa agctagctcc ttttttaact
    14701 tatatatgta aagcactatc ctaagggtct ttaatcatat gtcaataa
//
LOCUS       DANBWH010000053        14715 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.055, whole genome shotgun sequence.
ACCESSION   DANBWH010000053 DANBWH010000000
VERSION     DANBWH010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14715
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.055"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(161..1159)
                     /gene="sirC"
                     /locus_tag="PPY92_001093"
     CDS             complement(161..1159)
                     /gene="sirC"
                     /locus_tag="PPY92_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirC"
                     /protein_id="HDJ2625107.1"
                     /translation="MTEKINKKDNYHLIFALIFLAIVSVVSMMIGSSFIPLQRVLMYF
                     INPNDSMDQFTLEVLRLPRITLAILAGAALGMSGLMLQNVLKNPIASPDIIGITGGAS
                     LSAVVFIAFFSHLTIHLLPLFAVLGGAVAMMILLVFQTKGQIRPTILIIIGISMQTLF
                     IALVQGLLITTKQLSAAKAYTWLVGSLYGATFKDTIILGMVILAVVPLLFLVIPKMKI
                     SILDDPVAIGLGLHVQRMKLIQLITSTILVSMAISLVGNIGFVGLIAPHIAKTIVRGS
                     YAKKLLMSAMIGAISIVIADLIGRTLFLPKEVPAGVFIAAFGAPFFIYLLLTVKKL"
     gene            complement(1156..2151)
                     /gene="sirB"
                     /locus_tag="PPY92_001094"
     CDS             complement(1156..2151)
                     /gene="sirB"
                     /locus_tag="PPY92_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirB"
                     /protein_id="HDJ2625108.1"
                     /translation="MLLKPKYQIVIAGLCLAIVAILSLMIGNTLVSPGTVIQALFNFD
                     SENDLHDVVTGARASRTIIALLTGAALAVSGLLMQALTRNPIASPGLFGVNAGAVFFV
                     IFSITFIQIQSFKMIVVIAFLGAIVVTILVVALGMFRQTLFSPHRVILAGAAIAMLFT
                     AFTQGILIMNETDLQGLLFWLSGSVSLRNIWDIPWIIPLVLILILIAFSMAAHINILM
                     TSDDIATGLGQNIKLIKWMIIMLISMLAGISVAVAGSIVFVGLIVPNISKRLLPPNYK
                     YLIPFTALAGAILMIISDIVARIIIKPLELPIGVVTAVIGAIVLIYIMKKGRQRL"
     gene            complement(2167..3159)
                     /gene="sirA"
                     /locus_tag="PPY92_001095"
     CDS             complement(2167..3159)
                     /gene="sirA"
                     /locus_tag="PPY92_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter
                     substrate-binding protein SirA"
                     /protein_id="HDJ2625109.1"
                     /translation="MNKVIKMLVVTLAFLLVLAGCSGNSNKQSSDNKDKETTSIKHAM
                     GTTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVESWTQKPKFEYIKNDLKDTKIVGQ
                     EPAPNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALG
                     KEKEAEDLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILN
                     DLGFKRNKDLQKQVDNGKDIIQLTSKESIPLMNADHIFVVKSDPNAKDAALVKKTESE
                     WTSSKEWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLYEKLNIEKQSK"
     gene            3390..4370
                     /gene="sbnA"
                     /locus_tag="PPY92_001096"
     CDS             3390..4370
                     /gene="sbnA"
                     /locus_tag="PPY92_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="HDJ2625110.1"
                     /translation="MIEKSQACHDSLLDSVGQTPMVQLHQLFPKHEVFAKLEYMNPGG
                     SMKDRPAKYIIEHGIKHGLITENTHLIESTSGNLGIALAMIAKIKGLKLTCVVDPKIS
                     PTNLKIIKSYGANVEMVEEPDAHGGYLMTRIAKVQELLATIDDAYWINQYANELNWQS
                     HYHGAGTEIVETIKQPIDYFVAPVSTTGSIMGMSRKIKEVHPNAQIVAVDAKGSVIFG
                     DKPINRELPGIGASRVPEILNRSEINQVIHVDDYQSALGCRKLIDYEGIFAGGSTGSI
                     IAAIEQLITSIEEGATIVTILPDRGDRYLDLVYSDTWLEKMKSRQGVKSE"
     gene            4367..5377
                     /gene="sbnB"
                     /locus_tag="PPY92_001097"
     CDS             4367..5377
                     /gene="sbnB"
                     /locus_tag="PPY92_001097"
                     /EC_number="1.5.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate
                     dehydrogenase SbnB"
                     /protein_id="HDJ2625111.1"
                     /translation="MNREMLYLNRSDIEQAGGNHSQVYVDALTEALTAHAHNDFVQPL
                     KPYLRQDPENGHIADRIIAMPSHIGGEHAISGIKWIGSKHDNPSKRNMERASGVIILN
                     DPETNYPIAVMEASLISSMRTAAVSVIAAKHLAKKGFKDLTIIGCGLIGDKQLQSMLE
                     QFDHIKRVFVYDQFSEACARFVDRWQQQRPEINFIATENAKEAVSNGEVVITCTVTDQ
                     PYIEYDWLQKGAFISNISIMDVHKEVFIKADKVVVDDWSQCNREKKTINQLVLEGKFS
                     KEALHAELGQLVTGDIPGREDDDEIILLNPMGMAIEDISSAYFIYQQAQQQNIGTTLN
                     LY"
     gene            5398..7152
                     /gene="sbnC"
                     /locus_tag="PPY92_001098"
     CDS             5398..7152
                     /gene="sbnC"
                     /locus_tag="PPY92_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis protein SbnC"
                     /protein_id="HDJ2625112.1"
                     /translation="MQNHTAVNTAQAIILRDLVDALLFEDIAGIVSNSEITKENGQTL
                     LIYERETQQIKIPVYFSALNMFRYESSQPITIEGRVSKQPLTAAEFWQTIANMNCDLS
                     HEWEVARVEEGLTTAATQLAKQLSELDLASHPFVMSEQFASLKDRPFHPLAKEKRGLR
                     EADYQVYQAELNQSFPLMVAAVKKTHMIHGDTANIDELENLTVPIKEQATDMLNDQGL
                     SIDDYVLFPVHPWQYQHILPNVFATEISEKLVVLLPLKFGDYLSSSSMRSLIDIGAPY
                     NHVKVPFAMQSLGALRLTPTRYMKNGEQAEQLLRQLIEKDEALAKYVMVCDETAWWSY
                     MGQDNDIFKDQLGHLTVQLRKYPEVLAKNDTQQLVSMAALAANDRTLYQMICGKDNIS
                     KNDVMTLFEDIAQVFLKVTLSFMQYGALPELHGQNILLSFEDGRVQKCVLRDHDTVRI
                     YKPWLTAHQLSLPKYVVREDTPNTLINEDLETFFAYFQTLAVSVNLYAIIDAIQDLFG
                     VSEHELMSLLKQILKNEVATISWVTTDQLAVRHILFDKQTWPFKQILLPLLYQRDSGG
                     GSMPSGLTTVPNPMVTYD"
     gene            7145..8401
                     /gene="sbnD"
                     /locus_tag="PPY92_001099"
     CDS             7145..8401
                     /gene="sbnD"
                     /locus_tag="PPY92_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B export MFS transporter"
                     /protein_id="HDJ2625113.1"
                     /translation="MINQSIWRSNFRILWLSQFIAIAGLTVLVPLLPIYMASLQNLSV
                     VEIQLWSGIAIAAPAVTTMIASPIWGKLGDKISRKWMVLRALLGLAVCLFLMALCTTP
                     LQFVLVRLLQGLFGGVVDASSAFASAEAPAEDRGKVLGRLQSSVSAGSLVGPLIGGVT
                     ASILGFSALLMSIAVITFIVCIFGALKLIETTHMPKSQTPNINKGIRRSFQCLLCTQQ
                     TCRFIIVGVLANFAMYGMLTALSPLASSVNHTAIDDRSVIGFLQSAFWTASILSAPLW
                     GRFNDKSYVKSVYIFATIACGCSAILQGLATNIEFLMAARILQGLTYSALIQSVMFVV
                     VNACHQQLKGTFVGTTNSMLVVGQIIGSLSGAAITSYTTPATTFIVMGVVFAVSSLFL
                     ICSTITNQINDHTLMKLWELKQKSAK"
     gene            8391..10127
                     /gene="sbnE"
                     /locus_tag="PPY92_001100"
     CDS             8391..10127
                     /gene="sbnE"
                     /locus_tag="PPY92_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,3-diaminopropanoate--citrate ligase SbnE"
                     /protein_id="HDJ2625114.1"
                     /translation="MQNKELIQHAAYAAIERILNEYFREENLYQVPPQNHQWSIQLSE
                     LETLTGEFRYWSAMGHHMYHPEVWLIDGKSKKITTYKEAIARILQHMAQSADNQTAVQ
                     QHMAQIMSDIDNSIHRTARYLQSNTIDYVEDRYIVSEQSLYLGHPFHPTPKSASGFSE
                     ADLEKYAPECHTSFQLHYLAVHQDVLLTRYVEGKEDQVEKVLYQLADIDISEIPKDFI
                     LLPTHPYQINVLRQHPQYMQYSEQGLIKDLGVSGDLVYPTSSVRTVFSKALNIYLKLP
                     IHVKITNFIRTNDLEQIERTIDAAQVIASVKDEVETPHFKLMFEEGYRALLPNPLGQT
                     VEPEMDLLTNSAMIVREGIPNYHADKDIHVLASLFETMPDSPISKLAQVIEQSGLAPE
                     AWLECYLDRTLLPILKLFSNTGISLEAHVQNTLIELKDGIPDVCFVRDLEGICLSRTI
                     ATEKQLVPNVVAASSPVVYAHDEAWHRLKYYVVVNHLGHLVSTIGKATRNEVVLWQLV
                     AHRLMTWKKEYANNAVFVDCVEDLYQTPTIAAKANLMSKLNDCGANPIYTHIPNPICY
                     NKEVSYCESNNS"
     gene            10036..11886
                     /gene="sbnF"
                     /locus_tag="PPY92_001101"
     CDS             10036..11886
                     /gene="sbnF"
                     /locus_tag="PPY92_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(L-alanin-3-ylcarbamoyl)-2-[(2-
                     aminoethylcarbamoyl)methyl]-2-hydroxypropanoate synthase
                     SbnF"
                     /protein_id="HDJ2625115.1"
                     /translation="MIVVQTLFIHIYQIQFVITRRYRIVNQTILNRVKTRVMHQLVSS
                     LIYENIVVYKASYQDGVGHFTIEGHDSEYRFTAEKTHSFDRIRITSPIERVVGDEADT
                     TTDYTQLLREAVFTFPKNDEKLEQFIVELLQTELKDTQSMQYRESNPPATPETFNDYE
                     FYAMEGHQYHPSYKSRLGFTLSDNLKFGPDFVPNVKLQWLAIDKDKVETTVSRNVVVN
                     EMLRQQVGDKTYEHFVQQIEASGKHVNDVEMIPVHPWQFEHVIQVDLAEERLNGTVLW
                     LGESDELYHPQQSIRTMSPIDTTKYYLKVPISITNTSTKRVLAPHTIENAAQITDWLK
                     QIQQQDMYLKDELKTVFLGEVLGQSYLNTQLSPYKQTQVYGALGVIWRENIYHMLIDE
                     EDAIPFNALYASDKDGLPFIEKWIKQYGSEAWTKQFLAVAIRPMIHMLYYHGIAFESH
                     AQNMMLIHENGWPTRIALKDFHDGVRFKREHLSEAASHLTLKPMPEAHKKVNSNSFIE
                     TDDERLVRDFLHDAFFFINIAEIILFIEKQYGIDEQRQWQWVKDIIEAYQEAFPELNN
                     YQHFDLFEPTIQVEKLTTRRLLSDSELRIHHVTNPLGVGGINDATTISET"
     gene            11861..12637
                     /gene="sbnG"
                     /locus_tag="PPY92_001102"
     CDS             11861..12637
                     /gene="sbnG"
                     /locus_tag="PPY92_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis citrate synthase
                     SbnG"
                     /protein_id="HDJ2625116.1"
                     /translation="MQQLSLKHRLNNGDSVYGIFNSIPDPLMIEVIAASGYDFVVIDT
                     EHVAINDETLAHLIRAAEAAHIIPIVRVTAVIDRDIIKVLDMGARGIIVPHVKDRETV
                     EHIVKLSRYYPQGLRSLNGGRMARFGRTPLLDAMEMANEHIMVIAMIEDVEGVMAIDD
                     IAQVEGLDMIVEGAADLSQSLGIPWQTRDDQVTSHVQHIFEVVNAHGKHFCALPREDE
                     DIAKWQAQGVQTFILGDDRGKIYRHLSASLATSKQKGDDG"
     gene            12637..13839
                     /gene="sbnH"
                     /locus_tag="PPY92_001103"
     CDS             12637..13839
                     /gene="sbnH"
                     /locus_tag="PPY92_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis decarboxylase
                     SbnH"
                     /protein_id="HDJ2625117.1"
                     /translation="MRIVQPVIEQLKAQSHPVCHYIYDLVGLEHHLQHITSSLPSNCQ
                     MYYAMKANSERTILDTISQYVEGFEVASQGEIAKGLAFKPANHIIFGGPGKTDEELRY
                     AVSEGVQRIHVESMHELQRLNAILEDEDKTQHILLRVNLAGPFPNATLHMAGRPTQFG
                     ISEDEVDDVIEAALVMPNIHLDGFHFHSISNNLDSNLHVDVVKLYFKKAKSWSEKHRF
                     PLKHINLGGGIGVNYADLTSQFEWDNFVENFKTLIVEQEMEDVTLNFECGRFIVAHIG
                     YYVTEVLDIKKVHGAWYAILRGGTQQFRLPVSWQHNHPFEIYRYKDNPYSFEKVSISR
                     QDTTLVGQLCTPKDVFAREVQIDAISTGDVIVFKYAGAYGWSISHHDFLSHPHPEFIY
                     LTQTKEDE"
     gene            13843..14607
                     /gene="sbnI"
                     /locus_tag="PPY92_001104"
     CDS             13843..14607
                     /gene="sbnI"
                     /locus_tag="PPY92_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional
                     regulator/O-phospho-L-serine synthase SbnI"
                     /protein_id="HDJ2625118.1"
                     /translation="MNHIHEHLKLVPVDKIDLHETFEPLRLEKTKSSIEADDFIRHPI
                     LVTAMQHGRYMVIDGVHRYTSLKALGCKKVPVQEIHETQYSISTWQHKVPFGVWWETL
                     QQEHRLPWTTETRQEAPFITMCHGDTEQYLYTKDLGEAHFQVWEKVVASYSGCCSVER
                     IAQGTYPCLSQQDVLMKYQPLSYKEIEAVVHKGETVPAGVTRFNISGRCLNLQVPLAL
                     LKQDDDVEQLRNWKQFLADKFANMRCYTEKVYLVEQ"
BASE COUNT         4882 a         2414 c         2944 g         4475 t
ORIGIN      
        1 agtcggtgtt atttgtatat aaagtattaa tatgtaatta agaaagtcga cgcttgttaa
       61 caataacaac aactatatgt ataaaaaaac ctcgcaactg ttagttaact gaatcattta
      121 gctaacttcg ttgtgaggtc attttgtttt aataatatcg ttataacttt ttcacggtta
      181 ataataagta tatgaagaat ggggcaccaa aagcagcaat aaatacacct gctggcactt
      241 ctttaggcaa gaataaggta cgcccaatta agtctgcaat aacaattgat atggcaccaa
      301 tcattgctga cattagtaac tttttagcat aacttccgcg aacgattgtt ttcgcgatat
      361 gtggtgcgat taaaccgaca aacccaatgt tacctactaa actgattgcc atagatacga
      421 gtatagtaga agtgattaat tggattagtt tcatacgttg tacatgtaag cctaagccaa
      481 tcgctacagg gtcatcaagt atagatattt tcatttttgg tataacaaga aataacaacg
      541 gcacaacagc taaaataacc atacccaaaa tgattgtatc tttaaacgta gcaccgtaaa
      601 gacttccgac tagccatgta taagctttgg cagcagataa ttgcttcgtt gtaatgagta
      661 atccttggac aagcgcaata aacaacgttt gcatcgaaat accgatgatt atgagtattg
      721 tcgggcgtat ttgtcctttc gtttgaaaca ctaatagtat catcattgca actgcgccac
      781 ctaatactgc aaatagtgga agtaaatgta tcgttaaatg gctgaaaaat gcaataaaga
      841 caacagcact taagctagca ccacctgtga taccgataat atcaggtgag gcaattggat
      901 tttttaatac attttgcaac attaaaccac tcattcctag tgcggcacct gctaaaatcg
      961 caagtgtaat gcgaggtaag cgtaatactt ctaaagtgaa ttgatccata ctgtcatttg
     1021 gatttataaa gtacatcagt acgcgttgta atggtataaa gcttgaacca atcatcatac
     1081 ttaccactga aacgatggct aaaaagatta acgcgaagat gagatggtaa ttgtcttttt
     1141 tattaatctt ttcggtcata agcgttgacg tcctttcttc ataatataga ttaagacaat
     1201 agcgccaatg acagcggtaa cgacaccgat aggcaactct agtggcttaa ttattatacg
     1261 agcaacaatg tctgaaatga tcattaggat tgctccagct aatgcagtaa aaggaattaa
     1321 atacttatag tttggtggta ataatcgttt gctaatattc ggtacgataa gacccacaaa
     1381 gacgattgat ccagctacgg ctaccgaaat accggctaac atactgatga gcataataat
     1441 catccatttg attaatttta tgttttgacc gaggccggtt gcaatgtcgt cacttgtcat
     1501 caagatgttg atgtgtgcag ccatgctaaa tgcaattaaa ataagtatca atacaagcgg
     1561 aataatccat gggatatccc aaatattacg taatgaaacg gagccactta accaaaataa
     1621 taggccttgt aagtctgttt cgttcataat aagtatgcct tgagtaaagg ctgtaaatag
     1681 catcgcaatc gcagcacctg ccaaaatgac acggtgaggt gagaatagtg tttgtctaaa
     1741 catacctagc gcaacaacta atatagtaac aacaatggca cctaaaaatg caataactac
     1801 aatcatttta aaagattgaa tttggataaa tgtaatacta aaaatgacaa aaaatactgc
     1861 gcctgcattg acaccgaaaa gccctggtga ggctattggg tttcgtgtaa gtgcttgcat
     1921 caacaaacct gagacagcaa gggcagcacc agtcaataac gcaatgattg ttctcgacgc
     1981 ccgtgcacca gtgacaacat catgtaaatc gttttcacta tcaaagttga ataacgcctg
     2041 tatcaccgta cctggtgaca caagcgtatt tccaatcatt aaacttaaga tagctactat
     2101 tgcaagacat aaaccagcaa taacgatttg gtattttggt ttaagtagca tcgtaaaact
     2161 ccttaattat tttgattgtt tttcaatatt taacttttca tataaatcgt caataagttt
     2221 taatgaagat ttatatccgc cagctaagtt ccaagtgatt tcatctaaat catcagatac
     2281 ttggttgttt ttaactgcgt ctaaattttt ccactcttta cttgaagtcc attcgctttc
     2341 agtcttttta actaatgcag catctttcgc atttggatct gattttacta caaaaatatg
     2401 atcagcgttc attaatggaa tgctttcttt agatgtaagt tggataatat ctttaccatt
     2461 atcaacttgt ttttgtaagt ctttattacg tttgaatcct aaatcattta agatttcacc
     2521 agcatatcca ccagcataaa ttcttgtatg atcagcacgg aagttaacaa ctgaagcttt
     2581 caatggccat gcatctttat actttgcttt tgcatctttt tggaatgcag ctactttatc
     2641 atcgtacttt ttaagtaaat cttcagcttc tttttctttc cctaaagctt tccccattaa
     2701 cttagttgta tctttgaatt tgaaaactgt atcagtagaa actgttggtg cgattttaga
     2761 taattgatcg taaacttttt catttctaac ttttgacgcg acaattaagt ccggttttaa
     2821 tttagagatt tcctctaagt taggtgcagg ttcttgacct acaatcttag tatcttttaa
     2881 atcatttttt atgtattcga atttcggttt ttgtgtccat gattctacag cacctacagg
     2941 tttaacacct aaagatacag cgacgtcagt ggcaccttga tatagcgtaa caacacgctt
     3001 tggtttccct ttaatttcag ttgtacccat tgcatgttta attgaagttg tttccttatc
     3061 tttgttatca gatgattgtt tatttgaatt cccactacat cctgctaaaa caagtaggaa
     3121 agcaagcgta acaacaagca ttttaattac tttattcatt gactaattag cctccttcgt
     3181 gatgtatgac aatgagaatc attatcacga tttagtatga attaaatttt ttcctaagtc
     3241 aataaaatat ttatgattta catgcaactt ataattattt gacatataaa tgcataaaaa
     3301 atataatcct aattacttga tagtgagaat cattatcaat taggtaacac acaatattat
     3361 agaattttaa atttgaggag gaagcgcttt tgattgaaaa aagtcaagca tgtcacgatt
     3421 cattgttaga ttctgtaggg caaacaccta tggttcaact tcatcaacta tttccgaaac
     3481 atgaagtgtt tgcaaagtta gagtatatga atcctggagg cagcatgaaa gatcgacctg
     3541 ccaagtacat cattgaacat ggtattaaac atggtttaat cactgagaat acacatttaa
     3601 ttgaaagtac ttctggtaat ttaggcattg cgttggcaat gatagctaaa atcaagggat
     3661 taaaactcac gtgtgttgtt gatcctaaaa tatcaccaac aaatttgaaa attattaaaa
     3721 gttatggtgc caatgtagaa atggttgaag aacctgatgc acatgggggt tatttaatga
     3781 ctcgtattgc aaaggtgcaa gaactgttag ccactattga cgatgcatat tggattaatc
     3841 aatatgcgaa tgagttaaat tggcaatccc attatcatgg tgcaggcaca gagattgttg
     3901 aaacaattaa gcaacctata gattattttg tcgcgccagt cagcacgaca ggtagcatta
     3961 tgggtatgag tagaaaaata aaagaagtgc atccaaacgc acaaattgtt gctgttgatg
     4021 cgaaagggtc agtcattttt ggtgacaaac ctattaatag agaattacct ggtatcggtg
     4081 ctagtcgtgt acccgaaata ttgaatagat cagaaattaa tcaagtgatc catgtagatg
     4141 attatcaatc tgctttgggc tgtcgaaaac tgattgatta tgaaggcata tttgccggag
     4201 gttcaacagg ctcgattatt gcagcgattg agcagttgat aacgtcaatt gaagaaggtg
     4261 caacaattgt cacgatttta ccagatcgag gcgatcgtta cttagattta gtttattcag
     4321 atacatggtt agaaaaaatg aaatcaagac aaggagttaa atcagaatga atagagagat
     4381 gttgtattta aatagatcag atattgaaca agcgggaggt aatcattcac aagtttatgt
     4441 ggacgcatta acagaagcat taacagccca tgcgcacaat gattttgtac aaccgcttaa
     4501 gccgtattta agacaggatc ctgaaaatgg acacatcgca gatcgaatta ttgcaatgcc
     4561 aagtcatatc ggtggtgaac acgcaatttc aggtattaag tggataggta gtaagcacga
     4621 caatccatcg aaacgtaata tggagcgtgc aagtggtgtc attattttga atgatccaga
     4681 aacgaattat ccaattgcag ttatggaagc aagtttaatt agtagtatgc gtactgcagc
     4741 agtttcagtg attgcagcta agcatttggc taaaaaagga tttaaagact taacaatcat
     4801 tggttgcggg ctaatcggag acaagcaatt acaaagtatg ttagagcaat tcgatcatat
     4861 taaacgcgtg tttgtttacg atcaattctc tgaagcatgt gcacgctttg ttgatagatg
     4921 gcaacaacag cgtccggaaa ttaattttat tgcgacagaa aatgctaaag aagcagtatc
     4981 aaatggtgaa gtagtcatta catgtaccgt aacggatcaa ccatacattg aatatgattg
     5041 gttacaaaag ggtgcattta ttagcaacat ttctatcatg gatgtgcata aagaagtctt
     5101 tattaaagct gacaaagtcg tagtagatga ctggtcacaa tgtaatcgag aaaagaaaac
     5161 tattaaccaa ttggtgttag aaggtaaatt cagcaaagaa gcacttcatg ctgaactagg
     5221 gcaacttgtg acaggtgaca taccaggacg tgaagacgat gatgaaatca tattacttaa
     5281 tccgatgggt atggctatcg aagatatttc aagtgcttat tttatttatc aacaggcaca
     5341 acaacaaaat attgggacaa cattgaacct atattaagaa tgcgaggtgt ctgaacattg
     5401 cagaatcata cagcagtcaa tacagcacaa gcgataatat taagagattt agttgatgca
     5461 ttattatttg aagatatagc cggaattgta tcgaatagtg agattactaa agaaaacgga
     5521 caaacgcttt tgatatacga acgtgaaaca caacaaataa agatacctgt ttattttagt
     5581 gctttaaata tgtttcgtta cgaaagttca caaccaatta cgatagaggg aagggtgtct
     5641 aagcaacctt taacggcagc tgaattttgg caaacaattg ctaatatgaa ttgtgatcta
     5701 agtcatgaat gggaagtggc tcgcgttgaa gaaggactga ctactgctgc cacacagctt
     5761 gctaaacaat tatcagaatt agatttagcg tcacatcctt ttgtgatgtc agagcagttt
     5821 gcaagtttaa aagatcgtcc atttcatcca ttagctaaag aaaaaagagg attaagagaa
     5881 gcggattatc aagtgtatca agctgaatta aatcaatcat ttcctttaat ggttgcagca
     5941 gttaaaaaga cacatatgat tcatggcgat actgcaaata tcgatgaatt agaaaatttg
     6001 acagtaccta taaaagaaca agcgacagac atgttaaatg atcaagggtt atcaatagat
     6061 gactatgtac tatttccggt acatccttgg caatatcagc atattctgcc gaacgtcttt
     6121 gcgacagaga ttagtgaaaa gttggttgta ctattaccgt taaaatttgg agattatctg
     6181 tcgtcttcaa gtatgcgttc attaattgat attggcgcac cgtataacca tgtcaaagta
     6241 ccatttgcaa tgcagtcatt aggcgcatta aggctaacgc ctacgcgtta catgaaaaac
     6301 ggagaacaag cagaacaatt attacgtcag cttatagaaa aagatgaagc actagctaag
     6361 tatgtcatgg tttgtgatga aacagcttgg tggtcatata tgggtcaaga taatgatatt
     6421 ttcaaagatc aattaggtca tctaactgtt cagctaagaa agtatcccga agtgctagcc
     6481 aaaaatgata cgcaacagct agtgtcaatg gcagcactcg cggcaaatga tcgcacttta
     6541 tatcaaatga tttgtggaaa agataatatt tctaaaaatg atgtcatgac gttatttgaa
     6601 gatatcgcgc aagtcttttt aaaggtaaca ctatcattta tgcaatacgg cgcattacca
     6661 gagttgcatg gtcaaaatat attgttgtca tttgaagatg gacgtgtaca aaaatgcgtg
     6721 ttacgtgatc atgatactgt cagaatttat aaaccatggc taacagcaca tcagctttca
     6781 ttgccgaagt atgtcgtcag agaagataca cctaatacgc taattaatga ggatttggaa
     6841 acattctttg cttattttca aacattagct gtatcggtaa atctatatgc cattattgat
     6901 gcaattcaag atttatttgg tgtaagtgag catgaactta tgtcgttgtt aaaacaaatt
     6961 ttaaaaaatg aagtggcaac tatttcctgg gttacaactg atcagctagc tgtcagacac
     7021 attttatttg ataaacagac gtggccattc aaacaaattt tattaccatt gctatatcaa
     7081 cgtgatagtg gtggaggtag tatgccttca ggtttaacta ccgtaccaaa tccaatggtg
     7141 acatatgatt aatcagtcta tatggcgcag taactttcgc attttatggc tcagtcagtt
     7201 tatagcgatt gctggactga cagtacttgt gccattattg ccaatttata tggcatcact
     7261 acaaaatcta tcagtcgtag aaatacagtt gtggagtggt atagcgattg ctgctccagc
     7321 tgtaacgacg atgatagctt cgccgatatg ggggaagcta ggtgataaga tcagccgaaa
     7381 atggatggtc ttaagagcgt tacttggttt ggcggtatgc ttatttttaa tggcattgtg
     7441 tacgacacca ttacagtttg tacttgtgag gttattgcag ggactatttg gtggtgttgt
     7501 tgatgcatca agtgcgtttg cgagtgcaga ggcgccagct gaagatcgtg gaaaggtatt
     7561 aggaagactg caaagttcag tcagcgcagg gtctcttgtg gggccattaa ttggcggtgt
     7621 tacagcttcg atattaggtt ttagtgcgtt actgatgagt attgccgtta ttacttttat
     7681 tgtctgtatt ttcggtgcat taaaattgat tgaaacgaca catatgccaa aatcacaaac
     7741 accaaatatt aataaaggta ttcgccgttc atttcaatgt ctattatgca cacaacaaac
     7801 atgtcgattt attatcgttg gcgttttagc aaactttgct atgtatggca tgctaactgc
     7861 attatcacca cttgcttcat cagtgaatca tacagcgata gatgaccgta gtgtgattgg
     7921 atttttacag tccgcatttt ggacggcttc gatattaagc gcgcctttat ggggacgctt
     7981 taatgataaa tcatatgtta aatcagtata tatatttgcc acgattgcat gtggttgtag
     8041 tgcgatactg caaggtttag cgacgaatat agagttttta atggctgcaa gaatacttca
     8101 aggattaaca tatagtgcat tgattcaaag tgtcatgttt gttgtcgtga atgcgtgtca
     8161 tcaacaactt aaaggcacat ttgttggaac gacgaacagt atgttagttg ttggtcaaat
     8221 tattggcagt cttagtggcg ctgccattac aagttatact acaccagcta ctacgtttat
     8281 cgttatgggc gtagtatttg cagtaagtag tttattttta atttgttcaa ccatcactaa
     8341 tcaaatcaac gatcacacat taatgaaatt atgggagttg aaacaaaaaa gtgcaaaata
     8401 aagaattaat acaacatgca gcgtatgcgg ctatcgaacg cattttaaat gaatatttta
     8461 gagaagaaaa tttatatcaa gtaccacctc aaaatcatca atggtctata caattatcag
     8521 agctcgaaac tttaacgggt gaatttcgct attggtctgc gatggggcat catatgtatc
     8581 atccagaggt atggcttatc gatggaaaaa gtaaaaaaat aacaacttat aaagaagcaa
     8641 ttgcgcgtat tttgcaacat atggctcaaa gtgcagataa tcaaacggca gtgcaacaac
     8701 atatggcgca aattatgtct gacatcgata atagcattca tcgcacggcg cgttatttgc
     8761 aaagtaacac aatagactac gtagaggatc gttatatcgt ttcagaacaa tctttatact
     8821 taggtcatcc atttcatccg actcctaaga gtgcaagtgg gttttcagaa gcagatttag
     8881 agaaatatgc acccgaatgt catacatcat tccaattgca ttatttagct gtgcatcaag
     8941 atgttctgct cacgcgctat gtagaaggta aagaagatca ggttgagaaa gtgttgtatc
     9001 aattagcaga catagatata tcagagatac ccaaagattt tattttatta ccaacacatc
     9061 cttatcaaat caatgtgttg cgacagcatc cacagtatat gcaatatagt gaacaaggtt
     9121 taataaaaga ccttggcgtt tccggtgatt tagtgtaccc gacgtcttcg gttagaactg
     9181 tattttcaaa agcattaaac atttatttaa aattaccgat acacgttaaa attactaatt
     9241 ttatacgtac gaatgatctt gaacaaattg aacggacaat tgatgccgcg caagttatcg
     9301 catcagtcaa agatgaggtt gaaacacccc attttaaatt gatgtttgaa gaaggatatc
     9361 gtgcattgtt accgaatcca ttagggcaaa cagttgaacc tgaaatggat ttattaacaa
     9421 atagtgccat gattgttcgt gaagggatac cgaattacca tgctgataaa gatattcatg
     9481 tattggcgtc attatttgaa acgatgcctg attcaccgat ctctaagtta gcacaagtga
     9541 ttgagcaaag tggtttagca ccagaagcat ggcttgaatg ttatttggat cgtacattat
     9601 tgccgatatt aaagttgttt agtaacacag gcattagtct agaagcacat gtacaaaata
     9661 cattaattga attaaaagat ggcatacccg acgtatgctt tgtcagagat cttgaaggca
     9721 tttgtctatc tagaacgatt gctactgaaa aacagcttgt gccaaatgtt gtggcagcat
     9781 caagccctgt tgtatatgca catgatgaag catggcatcg tcttaaatat tatgttgtag
     9841 taaatcactt aggacattta gtatcaacta ttggtaaagc aactagaaat gaagttgtgt
     9901 tatggcaact tgtagcgcat cgtcttatga cttggaaaaa agaatacgcg aataacgcag
     9961 tatttgttga ctgtgtagaa gatttatatc aaacgccgac cattgcggct aaagcgaatt
    10021 tgatgagtaa attgaatgat tgtggtgcaa accctattta tacacatata ccaaatccaa
    10081 tttgttataa caaggaggta tcgtattgtg aatcaaacaa ttcttaatcg tgtaaagact
    10141 agagtaatgc atcaactggt atcatcactt atttatgaga atattgttgt gtataaagcg
    10201 tcatatcaag acggtgtcgg tcattttaca atagaaggac atgattcaga gtatcgtttt
    10261 actgctgaaa agacacatag ctttgatcgt atacgtatca catcaccaat tgagcgtgtc
    10321 gtaggagatg aggcagatac aacaacagac tatacacaat tattgagaga ggctgtattt
    10381 acatttccta aaaatgatga aaagctagaa caatttattg ttgagttatt acagacagaa
    10441 ttaaaagata cacaaagtat gcagtatcga gaatcaaacc caccagcaac acctgagaca
    10501 tttaacgact atgaatttta tgcgatggaa gggcatcagt atcatccaag ttacaaatca
    10561 cgtttaggat ttacgttgag tgataatttg aaatttggtc ctgattttgt accaaacgtt
    10621 aaactgcagt ggttagctat cgacaaagat aaagtagaaa cgacggtatc aagaaatgtt
    10681 gtagttaacg aaatgttacg tcaacaagtt ggcgataaga cttatgaaca ttttgtacag
    10741 caaattgaag cgtctggcaa acatgtaaat gatgttgaga tgatacctgt acacccatgg
    10801 cagtttgaac atgtcatcca agttgatttg gctgaagaaa ggcttaatgg cacagtacta
    10861 tggttagggg aaagtgatga gctatatcac cctcaacaat cgattcgtac gatgtcgcca
    10921 atagacacga caaaatatta tttaaaggta ccaataagta taacgaacac ttcaacgaaa
    10981 cgagtgttgg cgcctcatac aattgaaaat gcagcgcaaa ttacggattg gttaaagcag
    11041 atacagcaac aagatatgta tttaaaagat gaattaaaga cagtttttct aggggaagtc
    11101 ttaggacagt cttatttaaa tacacaactt tcgccttata aacaaactca agtttatggt
    11161 gcgttaggtg ttatatggcg tgaaaatata tatcatatgt taatcgatga agaggatgcg
    11221 ataccattta atgcacttta tgcaagtgat aaggatggtt taccattcat tgaaaagtgg
    11281 attaaacaat atggttctga agcttggaca aagcaatttt tagctgtagc gattcgtcca
    11341 atgattcata tgctttatta tcacggtatt gcctttgaat cgcatgcaca aaatatgatg
    11401 ctcattcatg aaaatggttg gcctacacgt attgccttaa aagatttcca cgatggtgtt
    11461 cgttttaagc gtgagcattt aagtgaagca gcttcacacc tgacattaaa gccaatgcca
    11521 gaagcacata aaaaagtgaa tagtaattca tttattgaaa cagatgacga acgtttagta
    11581 cgcgactttt tacatgatgc atttttcttt attaatatcg ccgaaatcat cttatttatt
    11641 gaaaagcaat atggtatcga tgagcagcga caatggcaat gggttaaaga cattatcgag
    11701 gcgtatcaag aagcatttcc agagttgaat aactatcaac atttcgattt gtttgaacct
    11761 acgattcaag ttgaaaagtt aacgacacgt cgattattaa gtgactccga gttaagaatt
    11821 catcatgtta caaatccatt aggtgtagga ggtatcaatg atgcaacaac tatctctgaa
    11881 acatagatta aacaatggtg attcagttta tggcattttt aattctatac cggacccatt
    11941 gatgatcgag gttatcgcag caagcgggta tgactttgtt gtgattgata cagaacacgt
    12001 ggcgattaat gatgagacac tagcgcattt aattcgtgca gctgaagcag cgcatattat
    12061 accaattgta cgtgtcactg cagtgataga tagagatatc attaaagtgt tagatatggg
    12121 tgcgagaggt attattgtgc cacacgttaa agatcgtgag acagttgagc atattgtgaa
    12181 attaagtcgt tattacccgc aaggattaag aagtttgaat ggtggtcgca tggcaagatt
    12241 tggacgtaca ccattacttg atgcaatgga gatggctaat gagcatatta tggtgattgc
    12301 catgatagaa gatgttgaag gggttatggc cattgacgat atagctcaag tcgaaggttt
    12361 agacatgata gtcgaaggtg ccgcagattt atcgcagtca cttggcatac catggcaaac
    12421 gcgtgatgat caagtaacat cacatgttca acatattttt gaggttgtga atgcacatgg
    12481 taaacatttt tgtgcattac cacgtgaaga tgaagatatt gcaaaatggc aggcacaagg
    12541 tgtacaaaca tttattttag gtgatgatcg cggaaaaata tatcgccatt taagtgcatc
    12601 tctagcgacg tctaaacaga aaggggatga tggctaatgc gtatagttca acctgttatt
    12661 gaacaattaa aagcacaatc tcatccagtt tgtcattata tctatgattt agtcggactg
    12721 gaacatcatt tgcaacatat tacatcgtca ttgccgagta attgtcaaat gtactatgca
    12781 atgaaagcaa atagtgaacg aacaatccta gatacaatta gtcagtatgt tgaaggattc
    12841 gaagttgcat ctcaaggtga aatagcaaaa ggtcttgctt ttaaaccagc aaatcatatt
    12901 atttttggtg gccctggtaa gacagacgag gaactaagat atgcagtaag tgaaggtgtt
    12961 cagcgtattc atgttgaaag tatgcatgaa ttacaacggc taaatgccat cttagaagat
    13021 gaagataaga cacaacacat tttattgcgt gttaatttag caggaccatt tcccaatgca
    13081 acgttgcata tggcaggacg cccaacacaa tttggtattt ctgaagacga agttgatgat
    13141 gtcattgaag ctgcgctagt aatgccaaat attcatctag atggctttca ttttcattct
    13201 atttctaaca atttagactc gaatttacat gtcgatgtag tgaaacttta ttttaaaaaa
    13261 gcaaaatcat ggtctgaaaa acatcgattt ccactcaaac atatcaatct tggtggtggc
    13321 ataggcgtta actatgcaga tttaactagc caatttgagt gggataattt tgtagaaaat
    13381 tttaaaacac ttatcgttga gcaagaaatg gaagatgtga cattgaactt tgaatgtggg
    13441 cgctttattg tggcacatat tggttactat gtgacagaag tgctagatat taagaaagtg
    13501 catggtgctt ggtatgccat tttaagagga ggtacgcaac aatttagact gccggtatct
    13561 tggcagcata accatccttt tgaaatttat cgttataagg acaatccata ttcatttgaa
    13621 aaagtttcaa tttcgagaca ggacacaacg ttagtcggtc aattatgtac accgaaagat
    13681 gtctttgcta gagaagtaca gatagacgca atcagtacag gcgacgttat tgttttcaaa
    13741 tatgcaggtg catacggatg gtctatttca catcacgatt tcttaagcca tccacatcct
    13801 gaatttattt atttaacgca aacaaaggag gatgaataac tattgaatca tattcatgaa
    13861 catttaaaat tggtaccagt agataagatt gatcttcacg aaacattcga acctttaaga
    13921 ttggaaaaaa cgaaaagtag tattgaagca gatgatttta tacgtcatcc tattttagtg
    13981 acagcgatgc aacatggtag atatatggtt atagatggtg tgcatcggta tacaagtttg
    14041 aaagcgttag gatgtaagaa agttccagtg caagaaatcc atgaaacaca atattcaatt
    14101 agtacatggc aacataaagt tccatttggt gtgtggtggg aaacgttaca acaagaacat
    14161 cgcttgccat ggactactga gacaagacaa gaagcgccat ttattacgat gtgtcatggt
    14221 gatacagaac aatatttgta tacgaaagat ttaggcgaag cacattttca agtatgggaa
    14281 aaggttgtcg caagttatag tggttgttgt tctgtagaga gaattgcaca aggtacatat
    14341 ccttgtcttt ctcaacaaga tgtactcatg aagtatcagc cattgagtta taaggaaatt
    14401 gaagcggttg ttcataaagg ggaaactgtg ccagcaggtg tgacacgctt taatatttca
    14461 ggacgatgtc ttaatcttca agtaccactg gcattactta aacaagatga tgatgttgaa
    14521 caactgcgta attggaagca gtttttagca gataagtttg ccaatatgag atgctatact
    14581 gaaaaagtat acttggtgga gcaatagttt tactgtgatg ttgagggaaa tatgatgatt
    14641 tagcgtattg atagcgaaaa tataataaaa caatatagtg tggagaactt ttgatatttt
    14701 ataaatattg aagtt
//
LOCUS       DANBWH010000054        14549 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.056, whole genome shotgun sequence.
ACCESSION   DANBWH010000054 DANBWH010000000
VERSION     DANBWH010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14549
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.056"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(494..1189)
                     /locus_tag="PPY92_001105"
     CDS             complement(494..1189)
                     /locus_tag="PPY92_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDJ2625119.1"
                     /translation="MKTTLLVVEDDEAILHLIDVALTMNYYKVVTAKTGKEADFRLRT
                     EQPDIILLDLGLPDIDGLSLIQQFRDFVDTPIIVISARTEEQTIVEVLDRDANDYMTK
                     PFNIDELRARIRVALRMSRSTETIQSRFTNGPLTIDFDAKSVLVNEQSVHLTPNEFQL
                     LTILCHHVGKVLTYQVLLKSLYGYVNHTEMAALRVHMASLRKKLNYTQDPNTEKKLII
                     THPRIGYQMQHLE"
     gene            complement(1164..3833)
                     /locus_tag="PPY92_001106"
     CDS             complement(1164..3833)
                     /locus_tag="PPY92_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HDJ2625120.1"
                     /translation="MESTYKKRGKLTIYLGYSPGVGKTYEMLSNGIDQYQEGADIKIG
                     YIEPHQRPETNALVQQLPEISTSSRKFGSHTFHYIDVSKIIEANPDVVLIDELAHTNI
                     SKERHLKRYKDIEEILSHGIDVWTTLNIQHIESLSGQIALMTGIQVTERVPDQFITSA
                     DAFEVVDISPNMLIQRLKAGKVYKKERLETAFSNFFTYENLTELRELTLRTVADIMSQ
                     KGQQYKTKHTDITPHIAVAISGSIYNERVIREARRAAYKEHAKFTAVYIDVFETPSES
                     RKQDHYVHKNLMLAKALGAEIKVLYAQDIAKALTDWCDLAFVTKLVLGQSEQPRWKDY
                     FKQSLIEQINHVPHHFKLEIVPIHYIHSDKPTNTPKQAQKSSRLTVEIMKMLVIQTIC
                     VLLGIWVYNLDKSESSAIILLMFFIGIIVLSIWTQSYLIGFFASILNVFVFNYFFTVP
                     RFTLEMYRFEYPITFATSIFASIFTSAILKNLKHQHSLTERQLYRTNIMLQFNQSIKE
                     SYSIERLLNIAGDQIHQLLNQDVTVFLIQSKKVAASNSFGNRSISSSDKTHDAETLSW
                     VIENESRAGKLTDTFPGSKFLCIPIGTNPVKGVISIRFTDETYIDTYDNSILDSMLND
                     ITLAIENVDLLKQTRQSILKAEREATRSNFLHSISHDIRTPLTSIMGNLDMLKYHNEH
                     LNDQQQAELLTASYGEAQYLHTLVTNILSLTKLESSDIQIQRTPYLVEELLEEFEEGL
                     IRRHQANHVIIENEDDASLINIDSKLILQVLFNLIDNALKHAESHSEIKLHVQHETNK
                     IKFEMIDCGKGIPEEERQLIFNPYYSGDNFKDNKKDSLGLGLYLVQLILKQHNSELEY
                     QPNTPQGSIFYFYLTVNPIEMEGAMDENNIIGR"
     gene            4000..4080
                     /gene="kdpF"
                     /locus_tag="PPY92_001107"
     CDS             4000..4080
                     /gene="kdpF"
                     /locus_tag="PPY92_001107"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDJ2625121.1"
                     /translation="MITLLAVVVIALILFLFYALIWSEKF"
     gene            4101..5777
                     /gene="kdpA"
                     /locus_tag="PPY92_001108"
     CDS             4101..5777
                     /gene="kdpA"
                     /locus_tag="PPY92_001108"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit A"
                     /protein_id="HDJ2625122.1"
                     /translation="MSIVLFLIVFILLSLIVSRYLYSVALNVPSKIDVVFNPIEKLIY
                     QLIGTKLEHMSGKTYIKHFLLFNGLMGGLSFVLLLIQQWLFLNPNHNLNQSVSLAFNT
                     MASFLTNTNLQHYAGETDLSYLTQMCVITFLMFTSAASGYAVCIAMLRRLTGMTDVIG
                     NFYQDITRFIVRVLIPFALIISLFLISQGTPQTLKGNLVIETLSGVKQTIAYGPMASL
                     ESIKHLGTNGGGFLGANSSTPFENPTYWSNYAEALSMMLIPGSLVFLFGRMLKTKLQI
                     HPHAIMIFVAMFVMFIGFLVTCLYFEFAGNPVLHHLGIAGGNMEGKETRFGIGLSALF
                     TTITTAFTTGTVNNMHDSLTPLGGMVPMVLMMLNAVFGGEGVGLMNMLIYVMLTVFIC
                     SLMIGKTPSYLGMKIEGKEMKLIALSFLVHPLLILVFSALAFIVPGASDALTNPQFHG
                     VSQVLYEFTSSSANNGSGFEGLGDNTVFWNISTGIVMLLARYIPIVLQILIVSSLVNK
                     KTYQQHTQDVPINNLFFSSVLIIFIILLSGLTFLPDLMLGPIGEQLLLHA"
     gene            5796..7817
                     /locus_tag="PPY92_001109"
     CDS             5796..7817
                     /locus_tag="PPY92_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit B"
                     /protein_id="HDJ2625123.1"
                     /translation="MAETTKIFESHLVKQALKDSVLKLYPVYMIKNPIMFVVEVGMLL
                     ALGLTIYPDLFHQESVSRLYVFSIFIILLLTLVFANFSEALAEGRGKAQANALRQTQT
                     EMKARRIKQDGSYEMIDASDLKKGHIVRVATGEQIPNDGKVIKGLATVDESAITGESA
                     PVIKESGGDFDNVIGGTSVASDWLEVEITSEPGHSFLDKMIGLVEGATRKKTPNEIAL
                     FTLLMTLTIIFLVVILTMYPLAKFLNFNLSIAMLIALAVCLIPTTIGGLLSAIGIAGM
                     DRVTQFNILAKSGRSVETCGDVNVLILDKTGTITYGNRMADAFIPVKSSSFERLVKAA
                     YESSIADDTPEGRSIVKLAYKQHIDLPQEVGEYIPFTAETRMSGVKFTTREVYKGAPN
                     SMVKRVKEAGGHIPVDLDALVKGVSKKGGTPLVVLEDNEILGVIYLKDVIKDGLVERF
                     RELREMGIETVMCTGDNELTAATIAKEAGVDRFVAECKPEDKINVIREEQAKGHIVAM
                     TGDGTNDAPALAEANVGLAMNSGTMSAKEAANLIDLDSNPTKLMEVVLIGKQLLMTRG
                     SLTTFSIANDIAKYFAILPAMFMAAMPAMNHLNIMHLHSPESAVLSALIFNALIIVLL
                     IPIAMKGVKFKGASTQTILMKNMLVYGLGGMIVPFIGIKLIDLIIQLFV"
     gene            7833..8390
                     /locus_tag="PPY92_001110"
     CDS             7833..8390
                     /locus_tag="PPY92_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDJ2625124.1"
                     /translation="MQTIRKSLGLVLIMFVLCGFIFPLTVTALGQVLFPEQANGSLVK
                     QDGKVIGSKLIGQQWTEPKYFHGRISAVNYNMNANEVKESGGPASGGSNYGNSNPELK
                     KRVQETIKQEGKKISSDAVTASGSGLDPDITVDNAKQQVKRIAKERNIDASKINHLID
                     ENKQASPMADDYVNVLKLNITLDKL"
     gene            complement(8935..9804)
                     /locus_tag="PPY92_001111"
     CDS             complement(8935..9804)
                     /locus_tag="PPY92_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625125.1"
                     /translation="MISTSEHSIFKNPAKNDGLRVRTIEVSEKFTSSAQNSDTIKKVV
                     SQNNGHVLPIIAQFLLDNTNDVTSLFQKEDSVFYQLLSTESSNTGIRMIKHYATLTTT
                     AHILNQSLDLQLNVSSIKNYLLSYHNETVSERSLADKAMDTIVQFIAENLGKFSQQTR
                     LANMIQNFGILSLKDDYIEVKIIRNVFDKMLTDSQFEDTKNVIDALDNAGKLVSDRDR
                     KTTKRNAKDDTGKSTSLVFYQIKIDLDYADVLGLQSQGRDNVIQKHSNPSQFKNTNLE
                     EFMKSENTTLIVF"
     gene            complement(9814..10710)
                     /locus_tag="PPY92_001112"
     CDS             complement(9814..10710)
                     /locus_tag="PPY92_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HDJ2625126.1"
                     /translation="MEKILYQTDEFKLKPSGWYKTIPPKKDGGTEFEIMLSGPIAFTD
                     RFIDPATRKEKVFLSDLNNIELVEKASILTALQLPSLIEYGFTINEKHIRDLGFVLQQ
                     MRSTTPLSTIYSGVGMLHTLLGPLISLDQPYFSNEITNSTSIICDNKYDLIPKGNLSE
                     WLQMYKEEVHGNLSLELDVLFGVSSLVTAFLKYHNNVEFSGTIFSFTGQSSTGKSTAA
                     MLAASVAGNPTKGTENLFRSWNATRNALEGYLSGNYGVPIVLDELSAATFHDTTGLLY
                     SFAEGQGRQRANINGDVKTPKN"
     gene            complement(11829..12455)
                     /locus_tag="PPY92_001113"
     CDS             complement(11829..12455)
                     /locus_tag="PPY92_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625127.1"
                     /translation="MQLQTMSNSIFIKNLIFAINESLEYSETNSVMEVTFQKAIGFTQ
                     YNFEGEKLKLKDDLKELISLVGREEAVLIFVNRLNEGLYCILNQLIEWDNLNYQFAMY
                     KEAMHNYLFNTIEKLNDDTLFLLHQDGLITLPEHKYKTVAVKSTFGSRDFTFRFKFPE
                     ESDTVNTHQMLNKAISITGSLANTHLEQPQKYKAHFKKIPIETKNLTS"
     gene            12570..14078
                     /locus_tag="PPY92_001114"
     CDS             12570..14078
                     /locus_tag="PPY92_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HDJ2625128.1"
                     /translation="MNEEILREVADIFIGDDRDSIYDYKTGNELVRFFNHYFNKGDIY
                     QAPFPSRWLYVVKHLQTLIQERKINQFFTLILSNHYIKYELKIDEVEAAKQAAKALKL
                     FNKRLNHYGYYITGTNNARYFMDKDEDTESIGYGGYANIYLQKSTGLAVKKLKEEYLT
                     DSSIKSRFKREFDLTKSFDTNPLFINVFEFNESDYSYTMELADETLKDYIESKTISEL
                     EKVKIIMKILKAMSQAHSENKIHRDISSKNVLMFRGKVKISDLGLGKNLDEIHSHQTF
                     DTNGVGQYKYCAPEQMYSLKQADKQSDVFSLGRLINFIMTGNVVNNHHLFRGVSDKAT
                     NSSKEYRFEDANEMLKMLQRILEYHSSAKHVEKCQEKLKRGVFDDESEEFIMTRSDEQ
                     LCQMVLSSNNNEQACLIRYMQKNESSACDLIESINRKYQEFCGRFEDYDPFAKLAYMI
                     LCNNFSYRVNETAARVLNYVAWSVNRFSAQDLIKGLINRGVEPLIEEKLKDN"
     gene            complement(14328..14462)
                     /locus_tag="PPY92_001115"
     CDS             complement(14328..14462)
                     /locus_tag="PPY92_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625129.1"
                     /translation="MKLHWYIYYFLSISVILFMIIYHYLATSMTVDYEITVSIAKLLL
                     "
     gene            14426..14518
                     /locus_tag="PPY92_001116"
     CDS             14426..14518
                     /locus_tag="PPY92_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625130.1"
                     /translation="MTKNSKYTNEVSKEQFQEIENVNNKVKEFY"
BASE COUNT         4605 a         2242 c         2781 g         4921 t
ORIGIN      
        1 aagaaacata gaatggaatt gcaattggga agtatacgat tttaactgcg aatcatatgt
       61 tcaacgactt acaaatgcgt aaatcgataa cacattcaaa aatatataat ctgaaaattg
      121 atttactaag agaggattat tgtagttaca ggtgttaagt tgctccaata tggagttgtc
      181 gtcttgtttt atattaaaca agttttattc aaatacatga caatacgtaa aataacacta
      241 gaattatcaa tcactaaaat taaaacaaat gtatctattc aaacttttct ttagggttta
      301 cttgctgaat aatttaaaaa tgaagtatta ggtgtacatg ctttcagaat tgcttttgaa
      361 gaatattaaa agtataatct gaatacgatg acagaatgaa taggtaaact atgacaagaa
      421 gtcatcaagg ttatcaaata aatatatccc agcggttgag acgactcact gctgggattt
      481 tattgttagt tatttattct aagtgttgca tttggtagcc gattctagga tgtgtgatga
      541 ttaatttctt ttcagtgttt gggtcttgcg tataattcaa ctttttacgt aaagaggcca
      601 tatgtacacg taaggcagcc atttctgtat gattgacata gccatagagt gattttaata
      661 acacttgata ggttaatact ttgccgacat gatggcataa tatcgttagg agttggaatt
      721 cattcggtgt tagatgtacg gactgttcat tgacaaggac tgatttcgca tcgaaatcaa
      781 tggttaatgg accatttgtg aaacgacttt gaattgtctc agtagaacgt gacatacgta
      841 atgctactct gatacgtgct cttaactcat cgatattgaa aggcttagtc atataatcat
      901 tggcatcgcg atctaatact tcgacaatgg tttgttcctc tgttcgtgca ctaatcacaa
      961 taataggtgt gtccacaaag tctctgaatt gctgaatgag agataaacca tcaatatctg
     1021 gtaagcctaa atctaataag ataatatctg gttgttctgt tcttaggcga aagtccgctt
     1081 ctttccccgt cttcgccgta accactttat aataattcat agttagcgca acatcgatta
     1141 aatgtaaaat cgcttcatca tcttcaacga ccaataatgt tgttttcatc catggctccc
     1201 tccatttcaa ttggattaac agtcaaataa aaataaaaaa tactgccttg tggtgtattc
     1261 ggttgatatt ctaattcact gttgtgttgt ttcaagatga gttgtactaa atagagccct
     1321 aatcccaaac tatctttttt attgtcttta aagttgtctc ctgaataata gggattaaaa
     1381 atcaattgac gttcttcttc tggaatacct tttccgcaat ctatcatttc gaattttatt
     1441 ttgtttgttt catgttgaac gtgcagtttt atttcagaat gtgattctgc atgctttaaa
     1501 gcattatcga taaggttgaa tagcacttgc agtattaatt tactgtcgat attaatgagt
     1561 gaagcgtcat cctcattttc aataatgaca tgatttgctt ggtgtctgcg tatgaggcct
     1621 tcttcaaatt cttctagaag ttcttctact aaataagggg tgcgttgtat ttgaatgtca
     1681 gagctttcta acttagtcaa agataaaata tttgtgacta aggtatgcag atattgtgct
     1741 tcgccataag aagcagttaa gagttctgct tgttgttgat cgtttaaatg ctcgttatgg
     1801 tatttcagca tatctaagtt gcccataatg gaagtcagtg gtgttctaat atcatgtgaa
     1861 attgaatgca agaagtttga acgtgtggct tctcgttcag ctttcaatat ggattgtctg
     1921 gtttgtttta atagatccac attctctatt gccagggtaa tatcgttcaa catagagtct
     1981 aatattgaat tgtcataggt gtcgatgtaa gtttcgtcgg tgaagcggat agaaatcaca
     2041 cctttgactg gatttgtgcc aatgggaata cacaaaaatt tactgccagg aaaggtatcg
     2101 gttaatttac cggcacggct ttcattttca atgacccagc tcaatgtctc agcatcatgt
     2161 gtcttatctg agcttgaaat gcttctgttt ccaaatgagt tggaagcagc gactttctta
     2221 ctttgaatta agaataccgt gacatcctga ttgagtagct gatgaatctg atcaccagca
     2281 atatttaata agcgttcaat tgaataagat tctttaatgg actggttaaa ttgcagcata
     2341 atattggtcc gatataactg ccgttctgtt aaggaatgct gatgttttaa attctttaag
     2401 atggcactcg taaagatact agcaaaaata ctggtcgcaa acgtaatcgg atattcaaag
     2461 cgatacattt ctaaagtgaa tcttggcacc gtaaagaaat aattaaaaac aaacacattt
     2521 aagatagacg caaagaatcc gattaaatag gattgggtcc aaatagaaag cacgataata
     2581 ccgataaaga acatcagcaa gataatggca ctggattcgc ttttatctaa gttgtaaacc
     2641 catataccga gcaagacaca gatcgtttga attacaagca tcttcataat ctctacagtt
     2701 aaacgagagg atttttgagc ttgcttaggt gtatttgtag gtttgtctga atgaatataa
     2761 tgaatcggta caatttctaa cttgaaatgg tgtggtacgt gattaatttg ttcaatgagt
     2821 gactgtttaa agtaatcttt ccaacgtggc tgttctgact gtccaaggac tagcttcgtc
     2881 acaaaagcga gatcacacca atcggttaac gctttcgcaa tatcttgtgc atacaacact
     2941 ttgatttctg cacctaacgc tttggcgagc attagatttt tatggacata atgatcttgt
     3001 tttctgcttt ctgacggtgt ctcaaagaca tcaatataca cagctgtgaa tttagcatgt
     3061 tctttatagg cagcacgtct tgcttctcgt atgacccgtt cgttataaat actgccgctg
     3121 atagctaccg caatatgcgg tgtaatgtcg gtatgtttag ttttatattg ttgtcctttt
     3181 tgactcataa tatctgcgac agttctgagt gtaagttcac gcagctctgt cagattttca
     3241 tacgtaaaga aattagaaaa ggctgtttct aagcgttctt ttttatatac ttttcctgct
     3301 ttaaggcgct gaatcaacat atttggtgaa atatccacaa cttcaaaggc atctgctgac
     3361 gtaatgaatt ggtcgggcac acgttctgta acttgaatac ctgtcattaa cgcaatttgt
     3421 ccgcttagac tctcgatatg ttggatgttg agtgttgtcc agacatcgat accatgcgat
     3481 agaatttctt ctatatcctt ataacgtttt aaatggcgct cttttgaaat gtttgtatgt
     3541 gctagttcat caattaagac cacatctgga ttagcttcta tgattttaga gacatctata
     3601 tagtgaaagg tgtggctgcc aaatttacgg ctggaggtcg aaatttcagg caattgttga
     3661 accagtgcat tggtttcagg gcgttgatgg ggttcgatat aaccaatttt aatatctgca
     3721 ccttcttgat actgatcaat accatttgat aacatttcat acgttttacc tacccctggg
     3781 ctatagccta aataaatggt aagtttccct ctttttttat atgtactttc catgaggcac
     3841 cccctcttaa tcacatcata attaatatac aacatattat ccatttatct ttctgttttg
     3901 ttaatcatct ttatagtttc tttataactt tccttaaatg ttaatacttc gattagataa
     3961 ctcttgttaa gattttgatg aaagtaagga gggagcgcaa tgattacact attagctgtc
     4021 gttgtcatcg cattaatttt atttttattt tacgcattaa tttggagtga aaaattttaa
     4081 cagagaaaga gggaagcatc atgagtattg tgttgttttt gattgtattt atcttgctct
     4141 cactcattgt gagccgatat ttatattcag ttgctttaaa tgtgccatct aaaatagatg
     4201 ttgtttttaa tccgattgag aaattgattt atcaactgat tggcacgaaa ttagaacaca
     4261 tgtctgggaa gacgtatatc aaacattttt tgttgtttaa cggattgatg ggcggattgt
     4321 cctttgtatt attgcttatt caacaatggc tgtttttgaa tcctaaccat aatttaaatc
     4381 aatctgtatc gttagccttt aatactatgg catctttttt gaccaatact aacttacagc
     4441 attatgcagg tgaaacagat ttaagttatt taacacaaat gtgtgtcatc actttcttaa
     4501 tgttcacgtc agcagcgtca ggttacgccg tatgtattgc gatgttaaga cgtttgactg
     4561 gaatgacaga tgtgattggt aatttctatc aagatattac gcgttttatt gtacgggtgc
     4621 tcataccttt cgcattgatc atcagtttgt ttttaatcag tcagggcaca ccgcaaacgc
     4681 ttaaaggtaa tttggtgatt gagacattat caggtgtgaa acaaacgatt gcatatggac
     4741 cgatggcgtc tttagaatct attaaacatt tagggacaaa tggtggtggt ttcttaggtg
     4801 cgaactcttc tacacctttt gaaaatccga catactggtc taattacgct gaagctttaa
     4861 gtatgatgtt gattccaggt tcattagtct ttctattcgg tagaatgttg aaaactaaac
     4921 tacagattca tccgcatgcg attatgattt tcgttgcgat gtttgtaatg ttcatcggct
     4981 ttttagtgac atgtctctat tttgaatttg cggggaatcc agtgttgcat cacttaggta
     5041 ttgccggtgg caatatggaa ggcaaagaaa cacgtttcgg tattggctta tccgctttat
     5101 ttacaaccat tacgaccgct tttactacag gaacagttaa caatatgcac gatagtctta
     5161 caccgctagg cggcatggtt ccaatggtat taatgatgtt gaatgcagtt tttggcggtg
     5221 aaggtgttgg gctgatgaac atgttgattt atgtcatgtt aacggtcttt atctgtagtt
     5281 tgatgattgg gaaaacacca agttatttag gaatgaagat tgaaggtaaa gagatgaaac
     5341 tcattgcgct ttctttctta gtacatcctt tacttatttt ggttttttca gcactagctt
     5401 ttattgtgcc aggggcatca gatgcgttaa ctaatccgca attccacggt gtatcacaag
     5461 tgttgtatga gtttacatca tcttcagcga ataatggctc tggttttgaa ggattaggag
     5521 acaatacggt attttggaac atttcaacag gcattgtgat gttgcttgca cgatatattc
     5581 caatcgtttt acaaattttg attgtatcta gtttggtaaa taaaaagacc tatcagcaac
     5641 atactcaaga tgtaccgatt aataatttat ttttcagcag tgtattgatt atctttatta
     5701 ttttgttgag cggcttaacg ttcttacctg acttaatgct tggaccaata ggcgaacagc
     5761 ttttgctgca cgcatagata aaggaggatt agaaaatggc tgaaactact aaaatatttg
     5821 aatcacattt ggtcaaacag gctctaaaag acagtgtatt gaagctctat cctgtttata
     5881 tgattaaaaa tccgattatg tttgttgtag aagtgggcat gctgcttgcc ttaggattaa
     5941 ccatttatcc ggatttattt caccaagaaa gtgtatcacg gctatatgtg ttcagtatct
     6001 ttatcatatt attactgaca cttgtctttg cgaacttctc tgaagcatta gctgaaggtc
     6061 gcggtaaagc acaagccaac gctttacgcc aaacacaaac tgaaatgaag gcacgtcgta
     6121 ttaaacaaga cggcagttat gaaatgattg acgctagtga cctgaaaaaa ggacatatcg
     6181 tacgtgtcgc gacaggtgaa caaatcccaa atgacggtaa agttattaag ggcctcgcaa
     6241 cagtggatga atctgcgatt acaggtgaat ctgcacctgt aatcaaagaa agcggtggag
     6301 atttcgataa tgtaattgga ggaacttctg tagcttcaga ctggttagaa gttgagatta
     6361 cttcagaacc aggtcattca tttttagata aaatgattgg tttggttgaa ggggctacaa
     6421 gaaagaaaac acctaatgaa attgcgttat ttactttatt gatgacatta acgattatct
     6481 tcttggtcgt tattttaacg atgtatccat tggcgaaatt cctgaatttc aatttatcca
     6541 ttgcgatgct gattgctttg gctgtgtgtt taattccaac aaccattggg ggattattat
     6601 cggctatagg gattgcaggg atggatcgtg tgacacagtt taatatcttg gctaaaagcg
     6661 gacgttctgt agagacttgt ggtgatgtga atgtcttgat tttagataaa acaggtacca
     6721 ttacctacgg caaccgtatg gcagatgcgt ttattccggt gaaatcatca agctttgaac
     6781 gtttagttaa agcggcctat gaaagttcta tcgcagatga cacaccagag ggacgtagta
     6841 ttgtgaaatt agcttataaa caacatatcg acttaccgca agaggtcgga gaatatattc
     6901 cgtttactgc tgaaacacgt atgagcggtg tgaaatttac gacacgtgaa gtatataaag
     6961 gtgcaccgaa tagtatggtt aagcgtgtga aagaagcagg gggacatatt ccagttgatt
     7021 tagacgctct tgtcaaaggg gtgtctaaaa aaggtggcac accgctggtt gtgcttgaag
     7081 ataatgagat tttaggtgtt atttatttga aagatgtcat taaagatgga ctcgtagaac
     7141 gtttccgtga attacgtgag atggggattg aaacggtgat gtgtacagga gataacgaat
     7201 tgacagctgc gacaatagcg aaagaagcgg gtgtggatcg ctttgtggca gagtgtaaac
     7261 ctgaagataa aatcaatgtg attagagaag aacaagcgaa aggtcatatt gttgcgatga
     7321 cgggtgacgg tacgaatgac gcgccagctt tagcagaagc taatgtaggt ttggcaatga
     7381 actcaggaac catgagtgcc aaagaagcgg cgaatttaat tgatttagat tctaatccaa
     7441 ccaaactgat ggaagtcgtt ctaattggga aacaattatt aatgacacgt ggctcactca
     7501 ctacatttag tattgcgaat gacattgcga aatactttgc gattttacca gccatgttta
     7561 tggcggctat gcctgcgatg aatcatttga atattatgca tctgcattca cctgaatcag
     7621 cagtattatc tgcgttaatc tttaatgcgt tgattattgt attattgatt ccgattgcga
     7681 tgaaaggcgt gaaatttaaa ggtgcctcaa cgcaaaccat attgatgaaa aatatgttag
     7741 tttacggctt aggcggtatg atcgtgccat ttatcggcat taagctcatt gatctcatca
     7801 tccaactctt tgtctaaaag gaggacaaaa caatgcagac aataagaaaa agtttaggac
     7861 tagtactgat tatgtttgtt ttatgcggat ttatcttccc gctgactgtc acagcgcttg
     7921 gacaagtatt atttccagaa caagcaaacg gcagtttagt gaaacaagat ggcaaagtaa
     7981 ttggttcaaa gctcattgga caacaatgga cagaacctaa atatttccat ggacgtatca
     8041 gtgcagtcaa ttacaatatg aatgcgaatg aagtgaaaga aagtggcgga cctgcttcag
     8101 gcggctcaaa ctacggcaat tcaaatcctg aattgaaaaa aagagttcaa gagactatta
     8161 aacaagaagg aaaaaaaatt tcaagtgatg cggtgaccgc ttctggctct ggtttagacc
     8221 cagatattac ggttgacaat gcgaaacaac aagtaaaacg cattgcgaaa gaaagaaaca
     8281 tagatgcttc aaaaattaat caccttattg atgaaaacaa acaagcatca ccaatggcag
     8341 atgattatgt taatgtctta aaattgaata tcactttaga taaactctaa ataaacaggg
     8401 agtgaggtga gacatccatg tggttcatta gcattattat tttaatagca ttcctaatta
     8461 tattaatgat tgacgattta attaaaacat gacaacttca cgcaaaacaa tatgaattgg
     8521 atgtaataga gaagggagta ccaagaaata ctcagtttat tgaggataat tattagggga
     8581 gattagagaa gaattctcta ttcacgaaca ttactttaac agttgtaatc attggtttca
     8641 tcatatcgat taatagtaca gtcatcttga gattttcaac acactattat agctagctaa
     8701 gatactttta aattatgact tatttttcat catttacgat acatcattct cgataactta
     8761 ctatttgtta tggtatttat agatttaaag aaagtgaagg agattaagca atgtaacttg
     8821 gcttaatctc tttttgatta tatttattaa ggcaaagctt catttccctt aaatcaagta
     8881 tatcttctga ataagcaaat gaaatcgtga cgccaactac agtgataact tcgcctaaaa
     8941 cacaatgagt gtggtgtttt cacttttcat aaactcctct aagtttgtgt ttttaaactg
     9001 tgatggattt gaatgttttt gaattacgtt gtcgcgtcct tgggattgca atccaagaac
     9061 gtcagcataa tctaaatcaa ttttgatttg gtagaagaca agagaagtac ttttgcctgt
     9121 atcatctttc gcatttcttt ttgttgtctt tcgatcccga tcggaaacta attttcctgc
     9181 attatccaaa gcatcaataa catttttcgt gtcttcgaat tgactgtctg ttaacatctt
     9241 atcgaataca tttcggataa tttttacttc gatgtagtca tctttaagtg agaggatacc
     9301 aaagttttga atcatattag caagacgtgt ttgttgtgaa aacttgccca ggttttccgc
     9361 aataaactgt acgattgtat ccatcgcttt atctgcaaga gaacgttctg acaccgtttc
     9421 attatgatag cttaataaat agttctttat gctgctaacg tttaactgta ggtctagtga
     9481 ttgatttaaa atatgggctg ttgttgttag agttgcataa tgtttaatca ttcgaatacc
     9541 agtgttactg gattcagttg atagtaattg ataaaaaaca ctgtcttctt tttgaaaaag
     9601 ggaagtaaca tcattagtgt tgtctaatag gaattgagca attataggta aaacatgacc
     9661 attattttga ctaaccactt tttttatagt atctgagttt tgagcacttg atgtgaattt
     9721 ttcagacacc tcaatagtac gaacacgcaa accatcattt ttagctggat ttttgaaaat
     9781 actatgttca gaagttgaaa tcactgatgt atcctagttt ttaggtgttt tgacatcacc
     9841 atttatattt gctctttgac gtccttgtcc ctctgcaaat gaatatagaa gacctgttgt
     9901 atcatgaaat gttgccgctg ataattcatc taatactatg ggtacaccat aatttccgct
     9961 aagatatcct tctagtgcat ttcttgttgc attccaactc cggaataggt tttcagtacc
    10021 ttttgttggg ttgccagcta ccgatgcggc taacatagcg gctgtcgatt ttcctgttga
    10081 tgattggcca gtgaaagaga aaatagttcc tgaaaactcg acgttattat gatacttaag
    10141 gaaagcagtg actaatgaag atacgccaaa caacacatct agttctaaag ataagttccc
    10201 atggacttct tctttataca tttgtaacca ttctgataaa ttgccttttg gtattaaatc
    10261 gtacttgtta tcacatataa tagaagtgct attagtaatc tcattagaaa aataaggctg
    10321 gtctaatgaa attaatggtc caagtaatgt atgaagcatt cctacacctg agtatattgt
    10381 tgataaaggt gtggtgcttc tcatttgttg cagtacgaac cctaaatctc taatgtgctt
    10441 ttcattaatg gtaaatccat attcaattaa ggagggtaat tgcaaagcag taaggattga
    10501 agccttttcg acaagctcta tattattcaa atccgaaaga aatacttttt ctttacgagt
    10561 cgcggggtct ataaaacgat ctgtgaaagc aatcggtcca gataacatta tttcaaactc
    10621 cgtaccccca tcttttttag gagggatggt tttgtaccat cccgaaggtt tgagcttgaa
    10681 ttcgtcagtt tgatatagaa ttttttccat taacagtgac ccccttggtt gtaagaggaa
    10741 attataatga tatgtcggta cttaagtggg ttagttccta taataatgaa agggatgctt
    10801 ggtttgtact ttatccacca agcaaagcgt cttcctgctt cacgttgatc gttgatgttg
    10861 cactgttctc tgaataactg taattataag gtttagtaat ggcaattttt tatttaaaca
    10921 aatttttacg aatggattgt ctgtttatta attcagcaac gtttattgaa gaaaaataag
    10981 tgtcttctga taattgcaaa gggtttaatt caatgtaaac tgaattaagg gtagggtagg
    11041 tatgcctgtt ttttggttct tttaagttca atcgattaat aaaattgttg aacctttgtt
    11101 ctaagtcata caaaaacatc agtaattcat taggagaatg atttattatg caatccatat
    11161 tttcgtttat gcttgaagtt atacatctaa aaacagtatt ggtttcttcg tataaaaata
    11221 acttattaat agaggtaaca ttgattaaga ctgagtatac gtataataag agtgactctg
    11281 acatctcatt tagataaata agactgtgat cattcttatt tacgattatt atatgtgatt
    11341 gaatcaattc aataatgtct agaagaagac ttgaaaagtc gctgttaatc ttggtctttg
    11401 aaaaatcatc gtgaccattt gtcttattac ttagatgtag ttgttcgtat tttatagttt
    11461 ggcggttgag gaattaagca tttgattatt ataaactatc tcaaaaatta aattacctcc
    11521 ttttttcttc ctcgatgaag gtttatctaa tcttccaata tacaggtttt tgaagtaaat
    11581 tatagatatg ataatcatta attcagtaaa tgtatactta ttaaggggtg gagctttagc
    11641 taacttatcg atagtactgc cgatgcgtgc tttgaaacta ttcaaattgt cgtgactgtc
    11701 tatatctaaa tcttcaaatt tactagcaag ggtttgcaaa gtagaaatta caacttcttc
    11761 ttttgtataa tatttattga aaatatcatc ttctttatca tcgctttgga gttttaaagt
    11821 gcctggttct aagatgtcaa attttttgtt tcgattggta tttttttaaa atgtgccttg
    11881 tatttttggg gttgttccaa atgtgtattt gctagactac ctgtaattga tattgcttta
    11941 tttagcattt ggtgtgtatt aactgtatct gattcttctg gaaatttaaa tctaaaagta
    12001 aaatctctac taccgaatgt actttttacg gctacagttt tgtacttatg ctcaggcaat
    12061 gtaattaaac catcttgatg caggaggaaa agtgtgtcat catttaattt ctcaattgta
    12121 ttaaataagt aattatgcat agcctcttta tacatggcga attgataatt tagattatcc
    12181 cattctatta gttgatttaa aatacaatat agaccttcat tcaatcggtt tacgaatatc
    12241 aatacagctt cttctcgtcc aacaaggctt attaactcct tcaagtcatc ctttaatttt
    12301 aatttttctc cttcaaaatt gtattgagtg aagcctatag ccttttgaaa agtaacttcc
    12361 atgacagagt tggtttcaga atattctaac gattcattta ttgcaaaaat caaatttttt
    12421 ataaatatgg aattactcat ggtttgtaat tgcatatact acccaacttt cttatttact
    12481 ttaaaacaaa tataagctaa agtagttatt atcataaagg gaacgtacgt tcttatttag
    12541 ctgatttcta tgaagagagg ttatggatat tgaatgaaga aatcttacgc gaagtagcag
    12601 atatttttat aggtgatgac agagatagca tttatgatta taaaactggg aacgaattag
    12661 tgaggttttt taatcattac tttaataaag gggacatata tcaggctccg tttccatcta
    12721 gatggctata tgttgtgaaa catttgcaaa ctctgattca ggagagaaag atcaatcaat
    12781 ttttcacatt aattttaagc aatcactata ttaagtatga actgaaaatt gacgaggttg
    12841 aagcagcaaa gcaggctgct aaagcactta agttgttcaa caaaagatta aatcattatg
    12901 ggtactacat aacaggaact aacaatgcta gatattttat ggataaggat gaagatacgg
    12961 agtctattgg ttatggaggg tatgcgaata tttatttaca gaagtctaca ggtcttgctg
    13021 taaaaaaatt gaaagaggag tatcttactg attcttcaat taaaagtagg tttaagagag
    13081 aatttgatct cactaaatct tttgatacaa atccattgtt cattaatgtg tttgaattta
    13141 atgaatcaga ttattcatac acgatggagt tagctgatga aactttgaaa gattacattg
    13201 aaagcaagac aattagtgag ctagaaaaag taaagataat aatgaaaatt ttaaaagcga
    13261 tgagtcaagc acatagtgaa aataaaatac acagagatat cagttctaaa aatgtattaa
    13321 tgtttagagg aaaagtcaaa atatcagact taggattagg taaaaacctt gatgaaattc
    13381 attcgcatca aacctttgat acaaacggtg taggacaata taaatactgt gcaccggaac
    13441 agatgtatag tttaaaacaa gcagataaac aatctgatgt ttttagttta ggaagattga
    13501 taaattttat tatgactgga aatgtagtta acaaccatca cctatttaga ggtgtatctg
    13561 ataaggctac gaacagtagt aaagaataca gatttgaaga tgcaaatgaa atgttgaaaa
    13621 tgctgcagag aattttagag tatcacagta gtgcaaagca cgtcgaaaaa tgtcaagaaa
    13681 agctgaaaag aggagtgttt gatgatgaaa gcgaagaatt tattatgaca cgaagtgatg
    13741 aacaattatg tcaaatggtt ctaagttcta ataataatga gcaagcgtgt ttaattcgtt
    13801 atatgcaaaa aaacgaatct tcagcatgtg atttaataga gagtattaat agaaagtatc
    13861 aagagttttg tggaaggttt gaagactacg atccttttgc taaattagca tatatgattt
    13921 tatgtaataa cttcagttat agagtgaatg aaacagcagc tagagtgcta aattatgttg
    13981 cttggtctgt aaatagattt tcggcacaag acttaattaa aggtttaatt aatagaggag
    14041 tggagccttt gattgaagaa aaattaaagg acaattaaaa aaacctcatc attaactgat
    14101 acgcagaggc gtatcataag taaaactaaa aaattctgta tgaggagata ataatttgga
    14161 gggtgttaaa tggtggacat taaatccacg ttcattcaat atataagata tatcacgata
    14221 attgcgcata taacttaagt agtagctaac agttgaaatt aggccctatc aaattggttt
    14281 atatctaaaa tgattaatat agaatgcttc tttttgtcct tattaaatta taaaagtaac
    14341 tttgcaatag aaacagttat ttcataatca acagtcattg acgtagctaa gtaatgataa
    14401 ataatcataa ataaaattac agatattgac aaaaaatagt aaatatacca atgaagtttc
    14461 aaaagaacaa ttccaagaaa ttgagaatgt aaataataag gtcaaagaat tttattaaga
    14521 tttgaaagag tatcaatcaa gaaagatgt
//
LOCUS       DANBWH010000055        14530 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.057, whole genome shotgun sequence.
ACCESSION   DANBWH010000055 DANBWH010000000
VERSION     DANBWH010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14530
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.057"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            494..1507
                     /locus_tag="PPY92_001117"
     CDS             494..1507
                     /locus_tag="PPY92_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDJ2625131.1"
                     /translation="MKDLLQAQQKLIPDLIDKMYKRFSILTTISKNQPVGRRSLSEHM
                     DMTERVLRSETDMLKKQDLIKVKPTGMEITAEGEQLISQLKGYFDIYADDNRLSEGIK
                     NKFQIKEVHVVPGDADNSQSVKTELGRQAGQLLEGILQEDAIVAVTGGSTMACVSEAI
                     HLLPYNVFFVPARGGLGENVVFQANTIAASMAQQAGGYYTTMYVPDNVSETTYNTLLL
                     EPSVINTLDKIKQANVILHGIGDALKMAHRRQSPEKVIEQLQHHQAVGEAFGYYFDTQ
                     GQIVHKVKTIGLQLEDLESKDFIFAVAGGKSKGEAIKAYLTIAPKNTVLITDEAAAKI
                     ILE"
     gene            1560..2570
                     /gene="gap"
                     /locus_tag="PPY92_001118"
     CDS             1560..2570
                     /gene="gap"
                     /locus_tag="PPY92_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDJ2625132.1"
                     /translation="MAVKVAINGFGRIGRLAFRRIQEVEGLEVVAVNDLTDDDMLAHL
                     LKYDTMQGRFTGEVEVVDGGFRVNGKEVKSFSEPDASKLPWKDLNIDVVLECTGFYTD
                     KDKAQAHIEAGAKKVLISAPATGDLKTIVFNTNHQELDGSETVVSGASCTTNSLAPVA
                     KVLNDDFGLVEGLMTTIHAYTGDQNTQDAPHRKGDKRRARAAAENIIPNSTGAAKAIG
                     KVIPEIDGKLDGGAQRVPVATGSLTELTVVLEKQDVTVEQVNEAMKNASNESFGYTED
                     EIVSSDVVGMTYGSLFDATQTRVMSVGDRQLVKVAAWYDNEMSYTAQLVRTLAYLAEL
                     SK"
     gene            2709..3899
                     /locus_tag="PPY92_001119"
     CDS             2709..3899
                     /locus_tag="PPY92_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDJ2625133.1"
                     /translation="MAKKIVSDLDLKGKTVLVRADFNVPLKDGEITNDNRIVQALPTI
                     QYIIEQGGKIVLFSHLGKVKEESDKAKLTLRPVAEDLSKKLDKEVVFVPETRGEKLEA
                     AIKDLKEGDVLLVENTRYEDLDGKKESKNDPELGKYWASLGDVFVNDAFGTAHREHAS
                     NVGISTHLETAAGFLMDKEIKFIGGVVNDPHKPVVAILGGAKVSDKINVIKNLVNIAD
                     KIIIGGGMAYTFLKAQGKEIGISLLEEDKIDFAKDLLEKHGDKIVLPVDTKVAKEFSN
                     DAKITVVPSDSIPADQEGMDIGPNTVKLFADELEGAHTVVWNGPMGVFEFSNFAQGTI
                     GVCKAIANLKDAITIIGGGDSAAAAISLGFENDFTHISTGGGASLEYLEGKELPGIKA
                     INNK"
     gene            4021..4782
                     /locus_tag="PPY92_001120"
     CDS             4021..4782
                     /locus_tag="PPY92_001120"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDJ2625134.1"
                     /translation="MRTPIIAGNWKMNKTVQEAKDFVNALPTLPDSKEVESVICAPAI
                     QLDALTTAVKEGKAQGLEIGAQNTYFEDNGAFTGETSPVALADLGVKYVVIGHSERRE
                     LFHETDEEINKKAHAIFKHGMTPIICVGETDEERESGKANDVVGEQVKKAVAGLSEDQ
                     LKSVVIAYEPIWAIGTGKSSTSEDANEMCAFVRQTIADLSSKEVSEATRIQYGGSVKP
                     NNIKEYMAQTDIDGALVGGASLKVEDFVQLLEGAK"
     gene            4785..6302
                     /locus_tag="PPY92_001121"
     CDS             4785..6302
                     /locus_tag="PPY92_001121"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDJ2625135.1"
                     /translation="MAKKPTALIILDGFANRESEHGNAVKLANKPNFDRYYNKYPTTQ
                     IEASGLDVGLPEGQMGNSEVGHMNIGAGRIVYQSLTRINKSIEDGDFFENDVLNNAIA
                     HVNSHDSALHIFGLLSDGGVHSHYKHLFALLELAKKQGVEKVYVHAFLDGRDVDQKSA
                     LKYIEETEAKFNELGIGQFASVSGRYYAMDRDKRWEREEKAYNAIRNFDAPTYATAKE
                     GVEASYNEGLTDEFVVPFIVENQNDGVNDGDTVIFYNFRPDRAAQLSEIFANRAFEGF
                     KVEQVKDLFYATFTKYNDNIDAAIVFEKVDLNNTIGEIAQNNNLTQLRIAETEKYPHV
                     TYFMSGGRNEEFKGERRRLIDSPKVATYDLKPEMSAYEVKDALLEELNKGDLDLIILN
                     FANPDMVGHSGMLEPTIKAIEAVDECLGEVVDKILDMDGYAIITADHGNSDQVLTDDD
                     QPMTTHTTNPVPVIVTKEGVTLRETGRLGDLAPTLLDLLNVEQPEDMTGESLIKH"
     gene            6432..7736
                     /gene="eno"
                     /locus_tag="PPY92_001122"
     CDS             6432..7736
                     /gene="eno"
                     /locus_tag="PPY92_001122"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDJ2625136.1"
                     /translation="MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTG
                     EHEAVELRDGDKSRYLGKGVTKAVENVNEIIAPEIIEGEFSVLDQVSIDKMMIALDGT
                     PNKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDA
                     PIAFQEFMILPVGATTFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTE
                     DAVETIIQAIEAAGYKPGEEVFLGFDCASSEFYENGVYDYSKFEGEHGAKRTAAEQVD
                     YLEQLVDKYPIITIEDGMDENDWDGWKQLTERIGDRVQLVGDDLFVTNTEILAKGIEN
                     GIGNSILIKVNQIGTLTETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDELFETAKYDGIKSFYNLDK"
     gene            8075..8533
                     /locus_tag="PPY92_001123"
     CDS             8075..8533
                     /locus_tag="PPY92_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625137.1"
                     /translation="MADRTNKEIKTGRFIATASIVFSILLIIHYFVSLDNATAKALLN
                     LTNQNTSDKAIDYILNSFRFTGIMYILAYLAGFITFWNRHTYVWWFMFAVYVSNSLFT
                     LINLSITIQAIKAAHGAYLTLPILIVIIGSVALAIYMLVVSIKRKSTFNR"
     gene            8600..8833
                     /gene="secG"
                     /locus_tag="PPY92_001124"
     CDS             8600..8833
                     /gene="secG"
                     /locus_tag="PPY92_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDJ2625138.1"
                     /translation="MHTFLIVLLIIDCIALITVVLLQEGKSSGLSGAISGGAEQLFGK
                     QKQRGVDLFLNRLTIILSILFFVLMICISYLGM"
     gene            8949..9689
                     /locus_tag="PPY92_001125"
     CDS             8949..9689
                     /locus_tag="PPY92_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HDJ2625139.1"
                     /translation="MQIKLPKPFFFEEGKRAVLLLHGFTGNSSDVRQLGRFLQKKGYT
                     SYAPQYEGHAAPPEEILKSSPFVWFKDALDGYDYLVEQGYDEIVVAGLSLGGDFALKL
                     SLNRDVKGIVTMCAPMGGKTEGAIYEGFLEYARNFKKYEGKNQETIDNEMDHFKPTET
                     LKELSEALDTIKDQVDEVFDPILVIQVENDNMIDPQSANYIYDHVDSDDKDIKWYSQS
                     GHVITIDKEKEQVFEDIYQFLESLDWSE"
     gene            9723..12095
                     /gene="rnr"
                     /locus_tag="PPY92_001126"
     CDS             9723..12095
                     /gene="rnr"
                     /locus_tag="PPY92_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDJ2625140.1"
                     /translation="MNLKQSIEEIINQPEYEPMSVSDFQDALGLSSADSFRDLIKVLV
                     ELEQSGLIERTKTDRYQKKHSSKGHSKLIKGTLSQNKKGFAFLRPEDEDMEDIFIPPT
                     KINRALDGDTVIVEIHQSKGEHKGKIEGEVKSIEKHSVTQVVGTYSEARHFGFVIPDD
                     KRIMQDIFIPKGQSLGAVDGHKVLVQITKYADGSDNPEGHISAILGHKNDPGVDILSI
                     IYQHGIEIEFPDEVLQEAEAVPDHIENTEIKGRHDLRDELTITIDGADAKDLDDAISV
                     KKLANGNTQLTVSIADVSYYVTEDSALDKEAYDRATSVYLVDRVIPMIPHRLSNGICS
                     LNPHVDRLTLSCRMEIDASGRVVKHEIFDSVIHSDYRMTYDAVNQIITEKDPNIREQY
                     KEITPMLDLAQDLSNRLIQMRKRRGEIDFDISEAKVLVNEVGIPTDVQLRQRGEGERL
                     IESFMLIANETVAEHFSKLNVPFIYRVHEQPKSDRLRQFFDFITNFGIMIKGTGEDIH
                     PTTLQKVQEEVEGRPEQMVISTMMLRSMQQAHYDDVNLGHFGLSAEYYTHFTSPIRRY
                     PDLTVHRLIRKYLIEKSMDNKEVKRWEDKLPELAEHTSKRERRAIEAERDTDELKKAE
                     YMIQHIGDEFEGIVSSVANFGMFIELPNTIEGMVHIANMTDDYYRFEERQMALIGERQ
                     AKVFRIGDTVKVKVTHVDVDERLIDFQIVGMPLPKNDRSQRPARGKTIQAKTRGKSLD
                     KSKSDDKGRKKKGKQRKGKNQRNNDKSGNSKHKPFYKDKSVKKKARRKKK"
     gene            12117..12581
                     /gene="smpB"
                     /locus_tag="PPY92_001127"
     CDS             12117..12581
                     /gene="smpB"
                     /locus_tag="PPY92_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDJ2625141.1"
                     /translation="MAKKKSPGTLAENRKARHDYNIEDTIEAGIVLQGTEIKSIRRGS
                     ANLKDSYAQVKNGEMYLNNMHIAPYEEGNRFNHDPLRSRKLLLHKREIFKLGEQTREI
                     GYSIVPLKLYLKHGHCKVLLGVARGKKKYDKRQALKEKAVKRDVARDMKARY"
     gene            12672..13033
                     /gene="ssrA"
                     /locus_tag="PPY92_001128"
     tmRNA           12672..13033
                     /gene="ssrA"
                     /locus_tag="PPY92_001128"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(13137..13352)
                     /locus_tag="PPY92_001129"
     CDS             complement(13137..13352)
                     /locus_tag="PPY92_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625142.1"
                     /translation="MIINGWKLYNNSNINLNFIDNKNSIQAISQMTPAFWVYFEFDLQ
                     KEEIIDINPKLCLEINKDNKTIKLFDK"
     gene            complement(13364..14470)
                     /locus_tag="PPY92_001130"
     CDS             complement(13364..14470)
                     /locus_tag="PPY92_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015728767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDJ2625143.1"
                     /translation="MKHNLNLSHNIYKDTKRGTYYFRITYYDKSNTRKYITRKGFKQR
                     KDAVKKCNEMMDELEGVGQLNRLPFDKLVEEYIDWYSARRKTSSVKALKTHTNNHLLP
                     YFKSIDVFKMTTQDVMKFQNKKLKEGYSGDYLKKMHVYLVSLLNHAMKFHELKQNVAS
                     LVGNFEIESQKRLNYWTLEQFNQFYGALVTQQQKLFFKLLFYSGARKGEIRALTWRDV
                     NFEDEFIHINKTDYHGEVTAPKTKSAIRDIYLPTHMMDDIKDYLIWHKENNIYKDDYV
                     LFGTFFKAYSESTIDRWFTTALKVLDDQLPDGQTFPRIVIHELRHSHASMLVNHGASI
                     MVIAQRLGHADSNEVYNRYGHLYPSTQKEIVKYL"
BASE COUNT         4991 a         2220 c         2839 g         4480 t
ORIGIN      
        1 caacttgcac attattgtaa gctgactttc cgccagcttc tgtgttgggg ccccacccca
       61 acttgcattg cctgtagaaa ttggaaatcc aatttctcta tgttggggcc cctgacttta
      121 attggaaaaa gcttgttaca agtgcatttt cgttcggtca actactacta atgtgacttt
      181 tcggattcta gagcattgtt ttatgtctta gtctcaaata ataagcaaat gattagcgag
      241 tcagtataag ggtatacatt tgactacagc gaataaaata aaacgttttg ttgaataaca
      301 aatcgaaaat gtattgcaag cgctttatca aattatttag aaaatttaag ttttatgctt
      361 gcaatttttg aaatagaata gtactattgc aagtgtaaag aggttaattt ttgtcccacg
      421 cgggacttaa aaaggcaacc actggttgtg acatatcctt atttacattt ataaatataa
      481 ggaggaggta gtagtgaaag acttattgca agcacagcaa aagcttatac cggatctcat
      541 agataaaatg tataaacgtt tttctattct tactactatc tcaaaaaatc agcctgtcgg
      601 acgtcgaagt ttaagcgaac atatggatat gactgaacgt gtactgcgtt ctgaaacaga
      661 tatgcttaag aaacaagatt tgataaaagt taagcctacc ggaatggaaa ttacagctga
      721 aggtgagcaa ctgatttcgc aattgaaagg ttactttgat atctatgcag atgataatcg
      781 tctgtcagaa ggtattaaga ataaatttca aattaaggaa gttcatgttg ttcctggtga
      841 tgctgataat agtcaatctg ttaaaacaga attaggtaga caagcaggtc aattacttga
      901 aggcatatta caagaagacg cgatagttgc tgtaactggc ggatccacga tggcatgtgt
      961 tagtgaagca attcatttat taccatataa tgtattcttc gtaccagcca gaggtggact
     1021 aggcgaaaat gttgtctttc aggcaaacac aattgcagcc agtatggcac aacaagctgg
     1081 cggttattat acgacgatgt atgtacctga taatgtcagt gaaacaacat ataacacatt
     1141 gttgttagag ccatcagtca taaacacttt agacaaaatt aaacaagcaa acgttatatt
     1201 acacggcatt ggtgatgcgc tgaagatggc gcatcgacgt caatcacctg aaaaggtcat
     1261 tgaacaactt caacatcatc aagctgtcgg agaggcattt ggttattatt ttgatacaca
     1321 aggtcaaatt gtccataagg ttaaaacaat tggacttcaa ttagaagacc ttgaatcaaa
     1381 agactttatt tttgcagttg caggaggcaa atcgaaaggt gaagcaatta aagcatactt
     1441 gacgattgca cccaagaata cagtgttaat cactgatgaa gccgcagcaa agataatact
     1501 tgaataagag ataaaaagtt taatactttt taaatatcat tttaaaggag gccattataa
     1561 tggcagtaaa agtagcaatt aatggttttg gtagaattgg tcgtttagca ttcagaagaa
     1621 ttcaagaagt agaaggtctt gaagttgtag cagtaaacga cttaacagat gacgacatgt
     1681 tagcgcattt attaaaatat gacactatgc aaggtcgttt cacaggtgaa gtagaggtag
     1741 ttgatggtgg tttccgcgta aatggtaaag aagttaaatc attcagtgaa ccagatgcaa
     1801 gcaaattacc ttggaaagac ttaaatatcg atgtagtgtt agaatgtact ggtttctaca
     1861 ctgataaaga taaagcacaa gctcatattg aagcaggcgc taaaaaagta ttaatctcag
     1921 caccagctac tggtgactta aaaacaatcg tattcaacac taaccaccaa gagttagacg
     1981 gttctgaaac agttgtttca ggtgcttcat gtactacaaa ctcattagca ccagttgcta
     2041 aagttttaaa cgatgacttt ggtttagttg aaggtttaat gactacaatt cacgcttaca
     2101 caggtgatca aaatacacaa gacgcacctc acagaaaagg tgacaaacgt cgtgctcgtg
     2161 cagcggcaga aaacatcatc cctaactcaa caggtgctgc taaagctatc ggtaaagtta
     2221 ttcctgaaat cgatggtaaa ttagatggtg gtgcacaacg tgttcctgta gctacaggtt
     2281 cattaactga attaacagta gtattagaaa aacaagacgt aacagttgaa caagttaacg
     2341 aagctatgaa aaatgcttca aacgaatcat tcggttacac tgaagacgaa atcgtttctt
     2401 cagacgttgt aggtatgact tacggttcat tattcgacgc tacacaaact cgtgtaatgt
     2461 cagttggcga ccgtcaatta gttaaagttg cagcttggta tgataacgaa atgtcatata
     2521 ctgcacaatt agttcgtaca ttagcatact tagctgaact ttctaaataa ttttagtata
     2581 gtttttattc aaatacgcta gtgctcagaa ctatttagca ttaattaaag cttatgagta
     2641 agcggggagc acaaacgctt ctccgcttat ttttatataa aatttcctaa ttacaaggag
     2701 gaaacaccat ggctaaaaaa attgtttctg atttagatct taaaggtaaa acagtcctag
     2761 tacgtgctga ttttaacgta cctttaaaag acggtgaaat tactaatgac aaccgtatcg
     2821 ttcaagcttt acctacaatc caatacatca tcgaacaagg tggtaaaatc gtactatttt
     2881 cacatttagg taaagtgaaa gaagaaagtg ataaagcaaa attaacttta cgtccagttg
     2941 ctgaagactt atctaagaaa ttagataaag aagttgtttt cgtaccagaa acacgcggcg
     3001 aaaaacttga agctgctatt aaagacctta aagaaggcga cgtattatta gttgaaaata
     3061 cacgttatga agatttagac ggtaaaaaag aatctaaaaa tgatccagaa ttaggtaaat
     3121 actgggcatc tttaggtgat gtgtttgtaa atgatgcttt tggtactgcg catcgtgagc
     3181 atgcatctaa tgttggtatt tctacacatt tagaaactgc agctggattc ttaatggata
     3241 aagaaattaa gtttattggt ggcgtagtta acgatccaca taaaccagtt gttgctattt
     3301 taggtggagc aaaagtatct gacaaaatta atgtcatcaa aaacttagtt aacatagctg
     3361 ataaaattat catcggcgga ggtatggctt atactttctt aaaagcgcaa ggtaaagaaa
     3421 ttggtatttc attattagaa gaagataaaa tcgacttcgc aaaagattta ttagaaaaac
     3481 atggtgataa aattgtatta ccagtagaca ctaaagttgc taaagaattt tctaatgatg
     3541 ccaaaatcac tgtagtacca tctgattcaa ttccagcaga ccaagaaggt atggatattg
     3601 gaccaaacac tgtaaaatta tttgcagatg aattagaagg tgcgcacact gttgtatgga
     3661 atggacctat gggtgtattc gagttcagta actttgcaca aggtacaatt ggtgtatgta
     3721 aagcaattgc aaaccttaaa gatgcaatta cgattatcgg tggcggtgat tcagctgcag
     3781 cagcaatctc tttaggtttt gaaaatgact tcactcatat ttcaactggt ggcggcgcgt
     3841 cattagagta cctagaaggt aaagaattgc ctggtatcaa agcaatcaat aataaataat
     3901 aaagtgatag tttaaagtga tgtggcatgt ttgtttaaca ttgttacggg aaaacagtca
     3961 caagatgaca tcgtgtttca tcacttttca aaaatattta caaaacaagg agtgtcttta
     4021 atgagaacac caattatagc tggtaactgg aaaatgaaca aaacagtaca agaagcaaaa
     4081 gacttcgtca atgcattacc aacattacca gattcaaaag aagtagaatc agtaatttgt
     4141 gcaccagcaa ttcaattaga tgcattaact actgcagtta aagaaggaaa agcacaaggt
     4201 ttagaaatcg gtgctcaaaa tacgtatttc gaagataatg gtgcgttcac aggtgaaacg
     4261 tctccagttg cattagcaga tttaggcgtt aaatacgttg ttatcggtca ttctgaacgt
     4321 cgtgaattat tccacgaaac agatgaagaa attaacaaaa aagcgcacgc tattttcaaa
     4381 catggaatga ctccaattat ttgtgttggt gaaacagacg aagagcgtga aagtggtaaa
     4441 gctaacgatg ttgtaggtga gcaagttaag aaagctgttg caggtttatc tgaagatcaa
     4501 cttaaatcag ttgtaattgc ttatgagcca atctgggcaa tcggaactgg taaatcatca
     4561 acatctgaag atgcaaatga aatgtgtgca tttgtacgtc aaactattgc tgacttatca
     4621 agcaaagaag tatcagaagc aactcgtatt caatatggtg gtagtgttaa acctaacaac
     4681 attaaagaat acatggcaca aactgatatt gatggggcat tagtaggtgg cgcatcactt
     4741 aaagttgaag atttcgtaca attgttagaa ggtgcaaaat aatcatggct aagaaaccaa
     4801 ctgcgttaat tattttagat ggttttgcga accgcgaaag cgaacatggt aatgcggtaa
     4861 aattagcaaa caagcctaat tttgatcgtt attacaacaa atatccaacg actcaaatcg
     4921 aagcgagtgg cttagatgtt ggactacctg aaggacaaat gggtaactca gaagttggtc
     4981 atatgaatat cggtgcagga cgtatcgttt atcaaagttt aactcgaatc aataaatcaa
     5041 ttgaagacgg tgatttcttt gaaaatgatg ttttaaataa tgcaattgca cacgtgaatt
     5101 cacatgattc agcgttacac atctttggtt tattgtctga cggtggtgtg cacagtcatt
     5161 acaaacattt atttgctttg ttagaacttg ctaaaaaaca aggtgttgaa aaagtttacg
     5221 tacacgcatt tttagatggt cgtgacgtag atcaaaaatc cgctttgaaa tacatcgaag
     5281 agactgaagc taaattcaat gaattaggca ttggtcaatt tgcatctgtg tctggtcgtt
     5341 attatgcaat ggaccgtgac aaacgttggg aacgtgaaga aaaagcttac aatgctattc
     5401 gtaattttga tgccccaact tatgcaactg ccaaagaagg tgtagaagca agctataatg
     5461 agggcttaac tgacgaattc gtagtaccat tcatcgttga gaatcaaaat gacggtgtta
     5521 atgatggaga tacagtgatc ttctataatt tccgacctga tagagcagca caattatcgg
     5581 aaatttttgc gaacagagca ttcgaaggct ttaaagttga acaagttaaa gacttattct
     5641 atgcaacatt cactaagtat aacgacaata tcgatgcggc tatcgttttc gaaaaagttg
     5701 atttaaataa tacaattggt gaaattgcac aaaataacaa tttaacacaa ttacgtattg
     5761 cagaaactga aaaatatcct cacgttactt actttatgag tggtggacgt aacgaggaat
     5821 ttaaaggtga acgccgtcgt ttaattgatt cacctaaagt tgcaacgtat gacttgaaac
     5881 cagaaatgag tgcttatgaa gttaaagatg cattattaga agagttaaat aaaggtgact
     5941 tggacttaat tattttaaac tttgctaacc ctgatatggt tggacatagt ggtatgcttg
     6001 agccgacaat caaagcaatc gaagcggttg atgaatgttt aggtgaagtc gttgataaga
     6061 ttttagacat ggacggttat gcaattatta ctgctgacca tggtaactct gatcaagtat
     6121 tgacggatga tgatcaacca atgactacgc atacaacgaa cccagtacca gtgattgtaa
     6181 caaaagaagg cgttacactt cgagaaactg gtcgcttagg tgacttagca cctacattat
     6241 tagatttatt aaatgtagaa caacctgaag acatgacagg tgaatcttta attaaacact
     6301 aatattgtaa aagatgttaa gtaaacgctt aatgacactt attttttgaa aataatagta
     6361 atatcatttt gttaaatgaa agaataaagc tataataatt atagaataac tatttaaagg
     6421 agattataaa catgccaatt attacagatg tttacgctcg cgaagtctta gactctcgtg
     6481 gtaacccaac tgttgaagta gaagtattaa ctgaaagtgg cgcatttggt cgtgcattag
     6541 taccatcagg tgcttcaact ggtgaacacg aagctgttga attacgtgat ggagacaaat
     6601 cacgttattt aggtaaaggt gttactaaag cagttgaaaa cgttaatgaa atcatcgcac
     6661 cagaaattat tgaaggtgaa ttttcagtat tagatcaagt atctattgat aaaatgatga
     6721 tcgcattaga cggtactcca aacaaaggta aattaggtgc aaatgctatt ttaggtgtat
     6781 ctatcgcagt agcacgtgca gcagctgact tattaggtca accactttac aaatatttag
     6841 gtggatttaa tggtaagcag ttaccagtac caatgatgaa catcgttaat ggtggttctc
     6901 actcagatgc tccaattgca ttccaagaat tcatgatttt acctgtaggt gctacaacgt
     6961 tcaaagaatc attacgttgg ggtactgaaa ttttccacaa cttaaaatca attttaagca
     7021 aacgtggttt agaaactgca gtaggtgacg aaggtggttt cgctcctaaa tttgaaggta
     7081 ctgaagatgc tgttgaaaca attatccaag caatcgaagc agctggttac aaaccaggtg
     7141 aagaagtatt cttaggattt gactgtgcat catcagaatt ctatgaaaat ggtgtatatg
     7201 actacagtaa gttcgaaggc gaacacggtg caaaacgtac agctgcagaa caagttgact
     7261 acttagaaca attagtagac aaatatccta tcattacaat tgaagacggt atggacgaaa
     7321 acgactggga tggttggaaa caacttacag aacgtatcgg tgaccgtgta caattagtag
     7381 gtgacgattt attcgtaaca aacactgaaa ttttagcaaa aggtattgaa aacggaattg
     7441 gtaactcaat cttaattaaa gttaaccaaa tcggtacatt aactgaaaca tttgatgcaa
     7501 tcgaaatggc tcaaaaagct ggttacacag cagtagtttc tcaccgttca ggtgaaacag
     7561 aagatacaac aattgctgat attgctgttg ctacaaacgc tggtcaaatt aaaactggtt
     7621 cattatcacg tactgaccgt attgctaaat acaatcaatt attacgtatc gaagatgaat
     7681 tatttgaaac tgctaaatat gacggtatca aatcattcta taacttagat aaataatcat
     7741 tttaaacatt aagtactgcc ataattttag ttgaggatta ttatgtcggt ataaattgaa
     7801 taaagttttt gagttcacgc ttaaaaagtt cacgcttgta tttataacct gccacagagt
     7861 tgagactgtg gtaggttttt tattttgaag tattattcat aacagactaa taatcatgag
     7921 gtaactaata acacatattt aacttgtatt cttaaactgg tataataaat ttatgttgaa
     7981 atgaatattg tatgacaggg tattcacttt tattaaaagg taaaattaaa taaaggtttt
     8041 atagaacgta tttaaatata tgaggagtaa acaaatggct gatagaacga ataaagaaat
     8101 taaaacagga cgctttattg caactgcatc aatcgtattc tcaatattat tgattattca
     8161 ttactttgtt tcgttggata atgcgactgc caaagcatta cttaatttaa cgaatcaaaa
     8221 cacttcagat aaagcgattg attacatttt aaacagcttt agattcactg gtattatgta
     8281 tattttggct tatctagcag gcttcatcac tttttggaat cgacatactt atgtgtggtg
     8341 gtttatgttt gcagtttatg tatcaaatag tttgtttacg ttgattaatt tatcaatcac
     8401 aattcaagca ataaaagctg cacacggtgc gtacttaaca ttgccaattt taatcgttat
     8461 tataggttcg gttgcattag cgatttatat gcttgttgtt tctatcaaac gtaaaagtac
     8521 atttaatcgc tagaaaattg attttaacaa taaaaatatg atatactact tgtcgtatat
     8581 aaggaacgga ggacaattta tgcatacatt tttaatcgta ttattaatca ttgattgtat
     8641 tgcattaata actgttgtac tactccaaga aggtaaaagc agtggacttt caggtgccat
     8701 cagtggtggt gctgagcagt tattcggtaa acaaaaacaa cgtggcgtcg atttattctt
     8761 aaatagatta acaattattt tatcaatatt attttttgta cttatgattt gcataagtta
     8821 tcttggtatg taaggtccgg cgatgtaaat gtcgggcttt tttatttata attaagaatg
     8881 taatagttta acaataagct atgtaaaata tatagcctag ttaagtatgc aaagggagcg
     8941 ttagatttat gcagataaaa ttaccaaaac ctttcttttt tgaagaaggc aaacgtgccg
     9001 tgttattatt acatggattt acaggcaatt cgtctgatgt acgtcaatta ggtcgatttt
     9061 tacaaaaaaa gggatatact tcgtatgcac cacaatatga aggtcacgcg gcaccaccag
     9121 aggaaatact gaaatctagt cctttcgttt ggtttaaaga tgcgttagat ggttatgatt
     9181 atcttgttga acaaggttat gatgaaattg ttgttgctgg tctatcatta ggtggggatt
     9241 ttgctttaaa attaagctta aatagagatg taaagggtat tgtaacgatg tgtgcaccaa
     9301 tgggtggcaa aactgaaggt gccatttatg aaggcttttt agagtatgca cgtaatttta
     9361 aaaagtatga aggtaaaaat caagagacta ttgataatga aatggatcat tttaaaccaa
     9421 ctgaaacttt aaaagaacta agtgaagcat tagatacgat taaagatcaa gttgatgagg
     9481 tatttgaccc aattttagtg attcaagtag aaaacgacaa tatgattgat ccacaatccg
     9541 caaattatat atatgaccat gtagattctg atgacaaaga catcaagtgg tatagtcaat
     9601 caggacatgt tattacgatt gataaagaaa aagaacaagt atttgaagat atttatcaat
     9661 ttttagagtc attagactgg tcagaataaa aagagatttt aacattagaa aggaggggca
     9721 taatgaattt aaagcaatca atagaagaga ttattaatca acctgaatat gaacctatgt
     9781 cagtgtcaga ttttcaagat gcattaggtt taagtagtgc cgactcattt agagatttaa
     9841 ttaaggtgct cgtagaatta gaacaatcag gattaattga acgtacaaaa acagacagat
     9901 atcaaaaaaa gcatagttca aaaggtcatt caaaattgat aaagggaacg ttaagtcaaa
     9961 ataaaaaagg ctttgcattc ttaagacctg aagatgagga tatggaagat atatttattc
    10021 ccccaacaaa aattaatcgt gccttagatg gagatactgt aattgtagaa atacatcaat
    10081 ctaaaggtga acataaaggt aaaattgaag gggaagttaa gtcgattgag aagcattcag
    10141 taactcaagt tgttggtacg tatagtgaag ctagacattt cggatttgtt attccagatg
    10201 ataaacgtat tatgcaagat attttcattc ctaaaggaca aagtttaggt gcagtagatg
    10261 ggcataaggt acttgttcaa attactaaat atgctgatgg ttcagataat ccagaaggac
    10321 atatttctgc tattttagga cataaaaatg atcctggcgt agatatttta tctattatct
    10381 atcaacatgg catagaaatt gaatttcctg atgaagtgtt acaagaagct gaagcagtac
    10441 ctgatcatat tgaaaatact gaaattaaag gccgtcatga tttacgtgat gaattgacaa
    10501 tcacaattga tggtgctgat gctaaagact tagatgacgc aattagtgtt aaaaagttag
    10561 cgaacggtaa tacgcagttg actgtaagta ttgctgatgt cagttattat gtaactgaag
    10621 attcagcttt agataaagaa gcttatgata gagcgacaag tgtatatctt gttgaccgtg
    10681 tgattccgat gattccacat cgattaagca atggtatttg ttcattaaat cctcatgttg
    10741 atcgtttaac gttaagctgt cgcatggaaa tcgatgctag tggtcgcgtt gttaaacatg
    10801 aaatttttga tagtgttata cattctgatt atcgaatgac gtatgatgcg gtaaatcaga
    10861 ttattactga aaaggatcct aacattcgcg aacaatataa agaaattacg cctatgttag
    10921 atttagcaca agatttatct aatcgtttga ttcaaatgag aaaacgacgt ggtgaaatcg
    10981 attttgatat tagtgaagca aaagtattag ttaacgaagt cggtatacca acagatgttc
    11041 aattaagaca acgtggcgag ggtgaacgtc taattgaatc atttatgtta attgcaaatg
    11101 aaacagttgc tgaacatttt agtaagttaa atgtaccttt tatttaccga gtgcatgagc
    11161 aacctaaatc agatcgctta agacaattct ttgattttat tacaaacttt ggcatcatga
    11221 ttaagggcac tggcgaagat attcatccaa caacacttca aaaggttcaa gaagaagtag
    11281 aaggtcgacc tgaacaaatg gtcatttcaa caatgatgtt acgttcaatg caacaagcgc
    11341 attatgatga tgtgaacttg ggacattttg gcttatcagc tgaatattat acgcatttta
    11401 catcaccaat tagacgttat cctgatttaa cagttcatcg tttaatccgt aagtatttaa
    11461 ttgagaaatc aatggataac aaagaagtga agcgttggga agacaaattg cctgagttag
    11521 ctgaacatac ttctaaacgt gaacgtcgtg ctattgaggc agaacgtgat actgatgaat
    11581 tgaaaaaagc agaatatatg attcaacata ttggtgatga atttgaaggt attgtcagct
    11641 cagtagctaa cttcggtatg ttcattgaat tgccaaatac gatagaaggt atggttcata
    11701 ttgcgaatat gactgatgat tattaccgtt ttgaagagcg tcaaatggca ttaattggtg
    11761 agcgtcaagc taaagtattt agaattggtg acacagttaa ggttaaagtg acgcatgttg
    11821 atgtagatga acgattaatt gattttcaaa ttgttggaat gcctttacct aaaaatgacc
    11881 gctcacagcg accagcaaga ggtaaaacga ttcaagctaa aacgcgtggc aaatctttag
    11941 ataaatcgaa atctgatgat aagggtcgta agaaaaaagg taagcaacgt aaaggtaaaa
    12001 accaacgtaa taatgataaa tcaggtaata gtaagcataa gccattttat aaagataaaa
    12061 gtgtgaaaaa gaaagcacgt cgtaagaaaa aataagcagc aatgaggtga gtatgaatgg
    12121 ctaagaagaa atcaccaggt acattagcgg aaaatcgtaa agcaagacat gattataata
    12181 ttgaagatac gattgaagcg ggaatcgtat tacaaggtac agaaataaaa tcgattcgcc
    12241 gaggtagtgc taaccttaaa gatagttacg cgcaagttaa aaacggtgaa atgtatttga
    12301 ataatatgca tatagcacca tacgaagaag ggaatcgttt taatcacgat cctcttcgtt
    12361 ctcgaaaatt attattgcat aaacgtgaaa tctttaaatt aggtgaacaa actcgagaaa
    12421 ttggttattc gattgtgccg ttaaagcttt atttgaagca tggacattgt aaagtattac
    12481 ttggtgttgc acgaggtaag aaaaaatatg ataaacgtca agctttgaaa gaaaaagcag
    12541 tcaaacgaga tgttgcgcgc gatatgaaag cccgttatta agcgatttag ttgcttaatc
    12601 gggctatatt tgatatagtt atatgtgctt ttgtaaatta caaaagtatg atttgtttga
    12661 tttattattt cggggacgtt catggattcg acaggggtcc cccgagctca ttaagcgtgt
    12721 cggagggttg gcttcgtcat caacacacac agtttataat aactggcaaa tcaaacaata
    12781 atttcgcagt agctgcctaa tcgcactctg catcgcctaa cagcatttcc tatgtgctgt
    12841 taacgcgatt caaccttaat aggatatgct aaacactgcc gtttgaagtc tgtttagaag
    12901 aaacttaatc aagctagcat catgttggtt gtttatcact tttcatgatg cgaaaccttt
    12961 cgataaacta cacacgtaga aagatgtgta tcaggacctc tggacgcggg ttcaaatccc
    13021 gccgtctcca ttataaagcc tacaaccaca tgggttgcag gctttttgtt tttgggtgca
    13081 caaaaagtgc acagatagtg cacagtacat aaaaaagaca cccatattta gggtgcttat
    13141 ttatcaaata gtttaattgt tttattatct ttatttattt ctaagcataa ctttggatta
    13201 atatctatta tttcctcttt ttgtaaatca aattcaaagt aaacccaaaa agctggtgtc
    13261 atttgactaa tggcttgtat gctattttta ttatctataa aatttaaatt gatatttgaa
    13321 ttattataaa gtttccaacc gttaattatc atatatcttc tccttacaaa tatttaacta
    13381 tttctttttg agtactaggg tataaatgtc catatctgtt gtatacttca ttgctatcag
    13441 catgtcctag cctttgagct attaccataa tactagcgcc gtgatttaca agcatagagg
    13501 catggctatg tcttaactcg tgtataacaa ttctagggaa tgtctgaccg tctggaagtt
    13561 ggtcgtctaa tacttttaat gcagtggtaa accaacgatc aatggttgac tcactataag
    13621 ccttaaagaa tgtaccaaat aatacgtaat catctttata tatgttattc tctttatgcc
    13681 aaattaaata atctttgatg tcatccatca tgtgagtagg caaatatata tcacgtatgg
    13741 ctgatttcgt tttaggggct gtcacttcac cgtgatagtc cgttttgttt atatggataa
    13801 attcatcttc aaaattaacg tcacgccatg tgagagccct aatttcgccc tttcttgctc
    13861 cagagtagaa caatagttta aagaataact tttgttgttg tgtcactagc gcaccataaa
    13921 attgattgaa ttgttctaac gtccaataat tcaatcgttt ctgtgattct atttcaaaat
    13981 tccctacaag agatgcaaca ttttgtttta actcatgaaa cttcattgca tgattcagta
    14041 atgatactaa atatacatgc atcttcttta agtagtctcc agaatacccc tcttttaact
    14101 tcttattctg aaatttcatc acatcttgtg tagtcatttt aaatacatct atagatttaa
    14161 aataaggtag caaatggtta tttgtatgtg tttttaatgc ttttacactt gatgtttttc
    14221 tacgcgctga ataccagtct atatattctt caacgagctt gtcaaaaggt aatctattaa
    14281 gctgtccgac tccctctaat tcgtccatca tttcgttgca tttcttaact gcgtctttac
    14341 gttgtttaaa ccccttacgt gttatgtact tacgagtatt gcttttgtca tagtaagtga
    14401 tacggaaata ataagtaccg cgtttagtgt ctttatatat gttgtgggac aggtttaagt
    14461 tatgttttat ggcaatcacc tatttcattt cttgttttta tgagtaagga taatttgatc
    14521 actgtatgtg
//
LOCUS       DANBWH010000056        14006 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.058, whole genome shotgun sequence.
ACCESSION   DANBWH010000056 DANBWH010000000
VERSION     DANBWH010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14006
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.058"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            27..1397
                     /locus_tag="PPY92_001131"
     CDS             27..1397
                     /locus_tag="PPY92_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HDJ2625144.1"
                     /translation="MNSDNMWLTVMGLIIIISIVGLLIAKKINPVVGMTIIPCLGAMI
                     LGYSVTDLVGFFAKGLDQVINVVIMFIFAIIFFGIMNDSGLFKPLVKRLILMTRGNVV
                     IVCAMTALIGTIAQLDGAGAVTFLLSIPALLPLYKALNMNKYLLILLLALSAAIMNMV
                     PWGGPMARVAAVLKAKSVNELWYGLIPIQIIGFILVMLFAVYLGFKEQKRIKKAIERN
                     ELPQTQDIDVHKLVEVYERDQDVRFPVKGRARTKSWIKWVNTALTLAVILSMLINIAP
                     PEFAFMIGVSLALVINFKSVDEQMERLRAHAPNALMMAAVIIAAGMFLGVLNETGMLK
                     AIATNLIKVIPAEVGPYLHIIVGLLGVPLDLLTSTDAYYFAVLPIVEQTAGQFGVPSV
                     STAYSMVIGNIIGTFVSPFSPALWLAIGLAEANMGTYIKYAFFWIWGFAIVMLVIAML
                     MGIVTI"
     gene            1587..3437
                     /gene="nrdD"
                     /locus_tag="PPY92_001132"
     CDS             1587..3437
                     /gene="nrdD"
                     /locus_tag="PPY92_001132"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDJ2625145.1"
                     /translation="MNQIEEALTGLISKDPAIVNENANKDSDTFSTMRDLTAGIVSKS
                     YALNYLLPKHVADAHQRGDIHFHDLDYHPFQPLTNCCLIDAKNMLHNGFEIGNANVTS
                     PKSIQTASAQLVQIIANVSSSQYGGCTVDRVDELLSTYARHNEEQHRNIAKQFVKESE
                     IDRYVDQQVTKDINDAIESLEYEINTLYTSNGQTPFVTLGFGLGTDHLSRKIQQAILN
                     TRIKGLGKDRTTAIFPKLVFSIKKGTNFSPQDPNYDIKQLALKCSMKRMYPDILNYDK
                     LVEILGDFKAPMGCRSFLPSWKDAEGHFENNGRCNLGVVTLNLPRMALESAGNMTKFW
                     EIFYERIDVLHDALLYRINRLKDAVPNNAPILYKSGAFNYKLKETDDVAELFKNKRAT
                     ISMGYIGLYETATVFYGPDWETSQEAKAFTLEILKEMKRYQTKWTELYDIWFSIYSTP
                     SESLTDRFCRLDQERFGDIKDITDKGYYQNSFHYDVRKDVTPFEKLDFEKDYPYYASG
                     GFIHYCEYPKLQHNLKALEAVWDYSYDKVGYLGTNIPIDHCYECDYDGDFEATEKGFK
                     CPNCGNDNPKTVDVVKRTCGYLGNPVQRPVIKGRHKEICARVKHMKAPKE"
     gene            3434..3970
                     /gene="nrdG"
                     /locus_tag="PPY92_001133"
     CDS             3434..3970
                     /gene="nrdG"
                     /locus_tag="PPY92_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDJ2625146.1"
                     /translation="MTLLDIKQGQGYIAKIESNSFVDGEGVRCSVYVSGCPFNCVGCY
                     NKASQKFRYGEKYTDEILAEILDDCDHDYISGLSLLGGEPFCNLDITLNLVKAFRARF
                     GNTKTIWVWTGFLYEYLANDCTERRELLSYIDVLVDGLFIQHLFKPDLPYKGSLNQRI
                     IDVQQSLSHARMIEYIVS"
     gene            4070..4180
                     /locus_tag="PPY92_001134"
     CDS             4070..4180
                     /locus_tag="PPY92_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625147.1"
                     /translation="MIEYDLTIILIPILKVAYPFFKKVERKKWYFSGKEV"
     gene            complement(4196..4357)
                     /locus_tag="PPY92_001135"
     CDS             complement(4196..4357)
                     /locus_tag="PPY92_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625148.1"
                     /translation="MAIIEYLYNFSRNKQNFNSAKTAVLSYDLTNLTNFTRTAFKNIY
                     TFLTNIYLL"
     gene            4489..6111
                     /locus_tag="PPY92_001136"
     CDS             4489..6111
                     /locus_tag="PPY92_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDJ2625149.1"
                     /translation="MKKEKVMDWTTFIGTVAVLLFAVIPMMAFPKASEDIITGINSAI
                     SDSIGSIYLFMGLAIFCFVMYIAFGKYGNVTLGKASDKPEFNTFTWAAMLFCAGIGSD
                     ILYWGVIEWAFYYQVPPNGAKSMSDEALQYATQYGMFHWGPIAWAIYVLPALPIGYLV
                     FVKKQPVYKISQACRPILKGQTDKFVGKVVDILFIFGLLGGAATSLALGVPLISAGIE
                     RLTGLDGKNMILRSAILLTITVIFAISSYTGLKKGIQKLSDINVWLSFVLLAFIFIIG
                     PTVFIMETTVTGFGNMLRDFFHMATWLEPFGGIKGRKETNFPQDWTIFYWSWWLVYAP
                     FIGLFIARISKGRRLKEVVLGTIIYGTLGCVLFFGIFGNYAVYLQISGQFNVTQYLNT
                     HGTEATIIEVVHHLPFPSLMIVLFLVSAFLFLATTFDSGSYILAAASQKKVVGEPLRA
                     NRLFWAFALCLLPFSLMLVGGERALEVLKTASILASVPLIVIFIFMMISFLIILGRDR
                     IKLETRAEKLKEVERRSLRIVQVSEEEQDDNL"
     gene            complement(6282..6845)
                     /locus_tag="PPY92_001137"
     CDS             complement(6282..6845)
                     /locus_tag="PPY92_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HDJ2625150.1"
                     /translation="MTRSQNKQQQLEQAKDIVINSIGQTMDLYGTNRSVGNLYGTMVF
                     EGSMTLDEMRHQLQMSKPSMSAGVKKLQEFDIVKQQFTRGSRKQHFIAEKDFFIFFRN
                     FFTKKFQREIDINVEAVKDAQAIINPLLESSDLTEAETQEAQKIKAQLDHTHVYYEWL
                     EQLTEAIESGEIFKYFPIPEQPSDSEN"
     gene            complement(6934..7029)
                     /locus_tag="PPY92_001138"
     CDS             complement(6934..7029)
                     /locus_tag="PPY92_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625151.1"
                     /translation="MKNVSIYKTTQELSFVSSLDFYLTKHLTLML"
     gene            7098..8588
                     /gene="betB"
                     /locus_tag="PPY92_001139"
     CDS             7098..8588
                     /gene="betB"
                     /locus_tag="PPY92_001139"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HDJ2625152.1"
                     /translation="MELLKHLSQRQYIDGEWVESTNKNTRDIINPYNQEVIFTVSEGT
                     KEDAERAILAARRAFESGEWSQETAEKRGKKVRAIADKIKEHREALARLETLDTGKTL
                     EESYADMDDIHNVFMYFAGLADKDGGEMIDSPIPDTESKIVKEPVGVVTQITPWNYPL
                     LQASWKIAPALATGCSLVMKPSEITPLTTIRVFELMEEVGFPKGTINLILGAGSEVGD
                     VMSGHKEVDLVSFTGGIETGKHIMKNAANNVTNIALELGGKNPNIIFDDADFELAVDQ
                     ALNGGYFHAGQVCSAGSRILVQNSIKDKFEQALIDRVKKIKLGNGFDADTEMGPVIST
                     EHRNKIESYMDVAKAEGATIAVGGKRPDRDDLKDGLFFEPTVITNCDTSMRIVQEEVF
                     GPVVTVEGFETEQEAIQLANDSIYGLAGAVFSKDIGKAQRVANKLKLGTVWINDFHPY
                     FAQAPWGGYKQSGIGRELGKEGLEEYLVSKHILTNTNPQLVNWFSK"
     gene            8849..10558
                     /gene="betA"
                     /locus_tag="PPY92_001140"
     CDS             8849..10558
                     /gene="betA"
                     /locus_tag="PPY92_001140"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HDJ2625153.1"
                     /translation="MSNKNKSYDYVIIGGGSAGSVLGNRLSEDKDKEVLVLEAGRSDY
                     FWDLFIQMPAALMFPSGNKFYDWIYSTDEEPHMGGRKVAHARGKVLGGSSSINGMIYQ
                     RGNPMDYEGWAEPEGMETWDFAHCLPYFKKLEKTYGAAPYDKFRGHDGPIKLKRGPAT
                     NPLFQSFFDAGVEAGYHKTPDVNGFRQEGFGPFDSQVHRGRRMSASRAYLHPAMKRKN
                     LTVETRAFVTEIHYEGRRATGVTYKKNGKLHTIDANEVILSGGAFNTPQLLQLSGIGD
                     SEFLKSKGIEPRVHLPGVGENFEDHLEVYIQHKCKEPVSLQPSLDIKRMPFIGLQWIF
                     TRTGAAASNHFEGGGFVRSNNEVDYPNLMFHFLPIAVRYDGQKAAVAHGYQVHVGPMY
                     SNSRGSLKIKSKDPFEKPSIRFNYLSTEEDKKEWVEAIRVARNILSQKAMDPFNGGEI
                     SPGPEVQTDEEILDWVRRDGETALHPSCSAKMGPASDPMAVVDPLTMKVHGMENLRVV
                     DASAMPRTTNGNIHAPVLMLAEKAADIIRGRKPLEPQYIDYYKHGVHDENEGAIEVKP
                     YAK"
     gene            10836..11048
                     /locus_tag="PPY92_001141"
     CDS             10836..11048
                     /locus_tag="PPY92_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HDJ2625154.1"
                     /translation="MSFYEYIQTFKDDKTPLGELAIWIKEDDSFPKQEKLTENILSYF
                     HQMSNIDHEFLEIVKRSLSLYDQLKS"
     gene            11328..11771
                     /locus_tag="PPY92_001142"
     CDS             11328..11771
                     /locus_tag="PPY92_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDJ2625155.1"
                     /translation="MNIYDEYRSYLIEELDDCLTIQKNNDTAYYDVLEAINDLSNDSL
                     CVLNHLYINEGQEETFEQKFLQRNKHLKNVDGFKALRFLRPRTAGRHYIIITLWENRQ
                     AFYHWQNSAEYKHTHKHRGTSKGADVKIINRELSYNIRIELADMV"
     gene            11965..13563
                     /locus_tag="PPY92_001143"
     CDS             11965..13563
                     /locus_tag="PPY92_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HDJ2625156.1"
                     /translation="MQKSALFAPEKYNIISEIEKYSHIPDKKAILYHNTEGEDISVTY
                     QQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRT
                     KDLQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVIAGQKDGWISLEAEKENASN
                     KLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWAT
                     AAPGWQKWVWSPFLSVLGSGATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMA
                     KLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDT
                     PQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPALFKGYYKEPERTAKAQ
                     TGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVA
                     KPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNS
                     GKIRRVELRDSEIEKWQQQKDSNQ"
     gene            complement(13623..13952)
                     /locus_tag="PPY92_001144"
     CDS             complement(13623..13952)
                     /locus_tag="PPY92_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625157.1"
                     /translation="MAYSILNLYSILIQYKCKINHKKEIQYLHPRKTLQLPLILKIIL
                     NLALIGIVIIVLTHTEFQLNAGDSNVFLLNWDIRGLTSYIFSFLILVALVSKLILIFI
                     TRRQRTS"
BASE COUNT         4806 a         2070 c         2733 g         4397 t
ORIGIN      
        1 tattaaaagt ggagggagaa aataatatga atagtgataa tatgtggtta acagtaatgg
       61 ggctcattat tattatttca attgtaggtt tactcattgc caaaaagata aatccagttg
      121 taggtatgac aatcatacct tgcttagggg caatgatttt aggatatagt gtgacagatt
      181 tggttggatt ttttgctaaa gggttagatc aagtcatcaa cgttgttatt atgtttatct
      241 ttgccattat tttctttggc atcatgaacg atagtggttt attcaagccg cttgtcaaac
      301 gcttaatatt aatgacacga ggcaatgtcg tcattgtctg tgcaatgaca gctttaattg
      361 gcacaatagc ccaattagat ggggccggtg cggtaacatt tttgctttct attcctgcat
      421 tattaccttt atataaagcg ttaaatatga ataaatattt attgatttta ctattagcat
      481 taagcgcggc gattatgaac atggtacctt ggggaggtcc aatggctcgt gtagctgcag
      541 tgttaaaagc caaaagtgtc aatgaattat ggtatggatt aatacctatt caaataatag
      601 gtttcattct tgttatgttg tttgcggtat atcttggatt taaagaacag aaacgtatca
      661 aaaaagcaat agagagaaat gaattaccgc aaacacaaga tatagatgta cataaattag
      721 ttgaagtata tgaacgagat caagatgtaa ggtttcctgt aaaaggacgt gcaagaacaa
      781 aatcatggat aaaatgggtg aatacagctt taactttagc tgttattcta tcgatgttaa
      841 taaatattgc gccacctgaa tttgcattca tgataggtgt ctcattggcg cttgttatta
      901 attttaaatc agtggatgaa caaatggagc gattaagagc gcatgcgccg aatgcattaa
      961 tgatggctgc agtgattatt gcagcaggta tgtttttagg tgtactaaat gaaaccggta
     1021 tgcttaaagc gattgcgacc aatttaatca aagtgattcc tgcagaagta ggaccatact
     1081 tgcatattat tgtaggttta cttggcgtac cattagattt actaactagc acagacgctt
     1141 attattttgc tgtgttaccg attgtcgaac aaacagcagg gcaatttggt gtaccgtctg
     1201 tatcaacagc ttattcaatg gtcataggga atattatagg tacatttgtc agcccatttt
     1261 caccagcctt atggttggca attggtttag cagaggcaaa catgggcacg tatattaagt
     1321 atgcattctt ttggatttgg ggattcgcca tcgttatgtt agtaattgca atgttgatgg
     1381 gcattgtgac gatttaagtt gcataaacat gtcgaaatga cagaacaatt ttaaaaatta
     1441 atgaaataat tgtgtagaaa tatgaatttc actaaatgtt aataactttg tgacgtttta
     1501 gttaacagac taataaaaat ttgaaaatac tatatatagt ggtataacgt aatgagtaga
     1561 cacaatatat aggaagaagg ggtaaaatga atcaaatcga agaagcatta acgggtttga
     1621 tttctaaaga tcctgctatt gttaacgaaa atgccaacaa agatagtgat acattttcaa
     1681 caatgagaga tttaacagca ggtatcgttt ctaaatctta cgcattaaat tatttattac
     1741 caaagcacgt tgcagatgca catcaaagag gggacataca ttttcacgac ttagattatc
     1801 atccattcca accgttaaca aactgttgtt taatagatgc taaaaatatg ctacataatg
     1861 gatttgaaat aggcaacgcg aatgtaactt caccaaaatc aatacaaact gcatcagcgc
     1921 agcttgtaca aattatagcc aatgtttcca gcagtcaata tggtggctgt acggttgacc
     1981 gcgttgacga attacttagt acatatgcac gacataatga agaacaacat agaaatatcg
     2041 caaagcaatt tgtcaaagaa tctgaaattg atcgttatgt tgatcaacaa gtcactaaag
     2101 acatcaatga tgcgattgaa agtctagaat atgaaattaa taccttatat acatctaatg
     2161 gacagacacc ttttgtaaca ttaggattcg gcttaggtac agatcattta agtcgcaaaa
     2221 ttcaacaagc tatcttaaat actcgtatca aaggcttagg aaaagaccgc acgacagcga
     2281 ttttcccaaa acttgtattt tcaattaaaa aaggaaccaa ctttagtccg caagatccga
     2341 actatgacat taaacaacta gcattaaagt gttcaatgaa acgtatgtat ccagatattt
     2401 taaattatga caaactcgta gaaatattag gtgatttcaa agcgccaatg ggttgtcgtt
     2461 catttttacc aagttggaaa gatgcggaag gtcattttga aaataatggt cgttgtaatc
     2521 ttggtgttgt tacacttaat ttacctagaa tggcattaga atctgccggt aatatgacga
     2581 aattctggga aatcttttat gaacgtatcg atgtgttaca tgatgcatta ctttatcgta
     2641 taaatcgttt gaaagatgct gtaccgaata acgcaccaat tttatataaa agtggcgctt
     2701 ttaactataa attaaaagaa acagatgatg ttgctgagtt atttaaaaat aaacgtgcaa
     2761 cgatttcaat gggctatata gggttgtatg aaacagctac tgttttctat ggtccagact
     2821 gggaaacatc tcaagaagca aaagcattta cgcttgaaat tcttaaagaa atgaaacgtt
     2881 atcaaacgaa atggacagaa ttatatgaca tttggttcag tatttacagt acgccgagtg
     2941 aatcgctaac ggatcgtttt tgtcgtttag accaagagag atttggagat attaaagaca
     3001 ttacagataa aggatattat caaaactctt tccattatga tgtacgtaaa gatgttacac
     3061 cttttgaaaa gttagatttt gaaaaagatt atccttatta tgcgagtggt ggtttcattc
     3121 actattgtga gtatccgaaa ttgcaacaca atttgaaagc actagaagcg gtatgggact
     3181 actcttatga caaagttggt tacttaggta caaatattcc gattgatcat tgttatgaat
     3241 gtgattacga tggagatttt gaagcaactg aaaaaggatt taaatgcccg aactgtggca
     3301 atgataatcc taaaacagtt gatgtcgtta aacgaacatg tggttactta ggcaatccag
     3361 ttcaacgtcc agtaattaaa ggccgtcata aagaaatttg cgcacgagta aaacatatga
     3421 aagcgcctaa agaatgacac ttttagacat taaacaagga caaggttata ttgctaaaat
     3481 agaatcaaat agctttgttg acggtgaagg agtaagatgc agtgtttatg tatcaggatg
     3541 tccatttaat tgtgttggat gttataacaa agcctcacaa aagttcagat atggcgagaa
     3601 atacactgat gaaatattag cagaaatatt agatgattgc gatcatgatt atatatctgg
     3661 gctaagtcta ttaggtggcg aaccattttg taatttggat attacattaa atcttgtcaa
     3721 agcatttcga gcacgttttg gaaatacaaa gacaatttgg gtatggactg gatttttata
     3781 tgaatattta gcaaatgatt gtacagaacg tcgagagtta ttatcataca ttgacgtttt
     3841 agtagatggt ctatttatac aacacttatt caaacctgat ttaccatata aaggttcttt
     3901 aaatcaacgc attatagatg tacaacaatc actctcgcat gcgcgtatga ttgaatatat
     3961 agttagttga atatgtatta gaagtcaagg taacattcgt tgccttggct tctttttagg
     4021 ttaggtacat aattgaaagt taataaaagc aattctttag aaaaatatat tgatagaata
     4081 tgacctaaca atcattttga taccaatact aaaagttgca tatccgtttt ttaaaaaagt
     4141 tgaaagaaaa aagtggtact ttagtgggaa ggaagtctaa ctttttggta gcgttttaca
     4201 ataaataaat attcgttaaa aacgtataaa tattcttaaa tgccgtccta gtaaaatttg
     4261 ttaaattcgt taaatcataa cttaacacag ctgttttagc gctattaaag ttttgtttat
     4321 tacgagaaaa attatataaa tattcaataa ttgccaagtt tcaaattgta tgaaatttgc
     4381 attattatta aatattagtt attgtcaatt ttgtgaatca atataattat tacattttga
     4441 gataaatcga aacaggattc ataaaattaa taattagggg gagcacaatt gaaaaaagag
     4501 aaagttatgg actggacgac ctttataggg acagtagctg tacttctttt tgcagttata
     4561 cctatgatgg cttttccaaa agcaagtgaa gatatcatca ctggtattaa tagtgccatt
     4621 tctgattcaa ttggttcgat atatttattt atggggctgg cgattttttg ctttgtaatg
     4681 tatatcgcat ttggtaaata tggtaatgtc acacttggta aagcaagtga taaaccagaa
     4741 tttaatacat ttacatgggc ggcaatgctg ttttgtgcag gcataggctc tgatatttta
     4801 tactggggcg ttattgaatg ggctttttac tatcaagttc caccaaatgg cgcgaaaagt
     4861 atgagtgatg aagcactcca atatgcgacg caatatggta tgttccactg gggaccaatt
     4921 gcttgggcta tttacgtttt accagcatta ccaattggtt atttagtgtt tgttaaaaaa
     4981 caaccggtgt ataaaattag tcaagcttgt cgtccgattt taaaaggtca aacagataaa
     5041 tttgtaggta aagttgtaga tatcttattt atctttggat tgctaggtgg tgcggcaaca
     5101 tcactagcgt taggtgtgcc attaatttct gcaggcatag aaagattaac tggtttagat
     5161 ggtaaaaata tgattttacg ttcggctatt ttattaacga ttacggtcat atttgctatt
     5221 agttcatata caggattgaa aaaaggtatt caaaagttaa gtgatatcaa cgtttggcta
     5281 tcatttgtac ttttagcctt tatatttatt attggaccga ctgtttttat tatggaaacg
     5341 acagtgacag ggttcggaaa tatgttgaga gattttttcc atatggcaac atggttagaa
     5401 ccattcggtg gtattaaagg tcgaaaagaa acgaatttcc cacaagactg gacaatattc
     5461 tactggtcat ggtggttagt atatgcgcca tttatcggtt tatttatcgc tagaatttca
     5521 aaaggtagac gccttaaaga agtcgtgcta ggaacaatta tttatggaac gcttggatgc
     5581 gtattattct ttggtatttt tggtaactat gctgtgtatt tacaaatttc tggacagttt
     5641 aatgtaacac aatatttaaa tacacatggt acagaggcaa ccattattga agtggtgcat
     5701 catttaccat tcccatcatt gatgattgta ctattcttag tatctgcttt cttattctta
     5761 gcaacaacat ttgattcggg ttcatatatt ttagcggcag catctcagaa aaaagtggta
     5821 ggcgaaccat tacgtgccaa tcgtttattc tgggcatttg cattgtgctt attgccattt
     5881 tcattgatgc tagttggtgg cgaacgtgca ttagaagtat tgaaaactgc ttcaatactg
     5941 gcaagtgtgc cattaattgt tatttttatt ttcatgatga tatcattttt aatcatttta
     6001 gggcgcgata gaattaaact tgaaacgcgt gctgaaaaat taaaagaagt tgaacgtcgt
     6061 tcattgcgaa tcgttcaagt atcagaagaa gaacaagacg ataatttata attcaaataa
     6121 tgatgcataa gattttgaat tttataagta tataattgat tgtcatataa aacaaaaacg
     6181 gtaatttcta tttattgaga tagaaattac cgtttatttc gtggacctat tgtattgttt
     6241 tcatcatgca taatcatcat tgccgctgtc cgaggctaat tttaattttc agaatcagaa
     6301 ggctgttctg gaattgggaa atatttgaaa atttcaccgc tttcaatcgc ttcggttaac
     6361 tgttctaacc attcgtaata aacatgtgta tgatcaagct gagctttaat tttttgtgcc
     6421 tcttgtgttt cagcttcagt taaatcactg ctttcaagta atggattgat aatagcttga
     6481 gcatctttta ctgcttcgac attgatgtca atttcacgct gaaatttttt agtgaaaaag
     6541 tttcggaaaa agatgaaaaa gtctttctcg gcgataaaat gttgtttgcg gcttcctctc
     6601 gtaaattgtt gtttaacaat atcaaattcc tgcaatttct taacgccagc actcatactt
     6661 ggtttgctca tttgcaattg atgacgcatt tcatcaagcg tcatactgcc ttcaaacacc
     6721 atagtgccat ataagtttcc tacacttcta ttagtgccat acaaatccat tgtctgtcca
     6781 attgaattaa ttacaatatc ttttgcttgt tctaattgtt gctgtttgtt ctgagaacga
     6841 gtcatcattg cacctccgta catcattttg ttcacgttaa aataaatact aatacattat
     6901 aaaacctttt ctaaaaaaag acattaaaaa tatttaaagc attaaagtta aatgtttcgt
     6961 taaataaaaa tctaacgaac ttacaaaact taattcttga gttgttttgt aaattgacac
     7021 atttttcatt tctatgctaa cataagtttg taaaattcgt taaataaaaa tttaacaaac
     7081 ttaacggagg ttgttgaatg gaacttttaa aacatttatc tcagcgtcaa tatattgatg
     7141 gtgagtgggt tgaaagcacg aataaaaata caagagatat tatcaatcct tacaatcaag
     7201 aagtgatatt tacggtttct gaagggacaa aagaggatgc agaacgtgca atcttagctg
     7261 caagacgtgc gtttgagtct ggtgaatggt cgcaagaaac tgctgaaaaa agaggtaaaa
     7321 aagtacgtgc catcgcggac aagatcaaag aacatcgcga agcgttagca cgattagaaa
     7381 cattagatac tggaaaaacg ttagaagaat catatgcaga tatggatgat attcataatg
     7441 tgtttatgta ttttgctgga ttagcagata aagatggtgg cgaaatgatt gattcaccaa
     7501 ttccagatac agaaagcaaa attgttaaag aaccagtagg tgtagttaca caaattacac
     7561 cttggaatta tccgttatta caagcatcat ggaaaattgc gccagcgctt gctacgggtt
     7621 gttcactagt tatgaaacca agtgaaatta caccattaac aacaatacgt gtttttgaat
     7681 taatggaaga agttggtttc cctaaaggaa caattaatct tattctaggt gcaggttctg
     7741 aagttggtga cgtaatgtca ggtcataaag aggttgacct tgtatcattt acaggtggca
     7801 ttgagactgg taagcatatt atgaaaaatg ctgctaataa tgttacgaat attgccttgg
     7861 aacttggcgg taaaaatcca aacattatct ttgatgatgc tgattttgaa ttggcagtag
     7921 accaagcgtt aaatggtgga tattttcatg caggtcaagt ttgttcagca ggatcaagaa
     7981 tattagtaca aaacagtatt aaagacaaat ttgagcaagc acttattgat cgcgtgaaaa
     8041 aaatcaaatt aggtaatggt tttgatgctg atactgaaat gggaccagtg atttcaacag
     8101 aacatcgtaa taagatcgaa tcttatatgg atgtagctaa agcagaaggc gcaacaattg
     8161 ctgttggtgg taaacgtcca gatagagacg atttaaaaga tggtctattc ttcgagccaa
     8221 cagtcattac aaattgtgat acgtcaatgc gtattgtaca agaagaggtt ttcggacctg
     8281 tcgttactgt agaaggcttt gaaactgaac aagaagcgat tcaattagcg aatgattcta
     8341 tatatggttt agcaggtgct gtattttcta aagatattgg aaaagcacaa cgcgttgcta
     8401 acaagttgaa acttggaacg gtgtggatta atgatttcca tccatatttt gcacaagcgc
     8461 catggggtgg atacaaacaa tcaggtatcg gtagagaatt aggcaaagaa ggcttagaag
     8521 agtaccttgt ttcaaaacac attttaacaa atacaaatcc acaattagtg aattggttta
     8581 gcaaataaaa attagataag gtgagtgcca ttgtaagaac acaagacact cactttgttt
     8641 tgtataagtg gcgaaatgtt gattgataat ttggactaaa cgcaaaatga atcatagatt
     8701 atttcatgac tgttagtaac aatcgtaaaa ggaaaatcga gtgttttggt tagccaagtt
     8761 tagcaattca acgataacca atcagccact aacaaatatt tcatgcaata ctcactttga
     8821 aatacaacaa actttggagg tcataacgat gagtaacaaa aataaatcat atgattatgt
     8881 catcattgga ggaggcagtg caggttctgt actaggtaat cgtctgagtg aagataaaga
     8941 taaagaagtc ttagtattag aagcgggtcg cagtgattat ttttgggatt tatttatcca
     9001 aatgcctgct gcgttaatgt tcccttcagg caataaattt tacgattgga tttattcaac
     9061 agatgaagaa ccacatatgg gcggtcgtaa agtagcacat gcccgaggta aagttttagg
     9121 tggatcaagt tcgattaatg gcatgattta tcaacgtggt aatccaatgg actatgaagg
     9181 ctgggcagaa ccagaaggta tggaaacttg ggattttgcg cactgtttac cgtattttaa
     9241 aaaattagaa aaaacatacg gtgcagcgcc ttatgataaa tttagaggcc atgatggacc
     9301 aattaagtta aaacgagggc cagcaacgaa tcctttattc cagtcattct ttgatgcagg
     9361 tgttgaagca ggttatcata aaacacctga tgtgaatgga tttagacaag aaggttttgg
     9421 accgttcgat agtcaagtac atcgtggtcg ccgaatgtca gcttcaagag catatttaca
     9481 tccagcgatg aagcgtaaaa acttaaccgt tgaaacacgt gcctttgtaa ctgaaattca
     9541 ttatgaaggt agaagagcaa ctggtgttac gtataagaaa aatggcaaac tacataccat
     9601 cgatgctaat gaagtcattt tgtctggtgg ggcattcaat acgccacaat tactacaatt
     9661 atctggtatc ggtgattcag agttcctaaa atcaaaaggc attgagccac gtgttcattt
     9721 accaggtgtg ggtgaaaact ttgaagatca cttagaggta tacattcaac ataaatgtaa
     9781 ggaacctgta tcattacagc caagcttaga tatcaagcgc atgccattta ttggtttaca
     9841 atggattttc acacgtacag gtgcagcagc atctaaccat tttgaaggtg gcggatttgt
     9901 acgttcaaat aacgaagttg attatcctaa cttaatgttc catttcttac caatagctgt
     9961 aagatatgat ggtcaaaagg cagcggtcgc acatggttat caagttcacg taggaccaat
    10021 gtattcaaac tctcgtggta gcttgaaaat caaatctaaa gatccattcg aaaaaccaag
    10081 tatccgcttt aattatttat caacagaaga agataaaaaa gaatgggtag aagcaattcg
    10141 tgtagcaaga aatatcttat ctcaaaaagc aatggatcca ttcaatggtg gagaaatttc
    10201 accaggacca gaagtgcaaa cagacgaaga aattcttgat tgggttcgta gagatggtga
    10261 aacagcatta cacccatctt gtagtgctaa aatgggtcct gcttcagatc caatggcagt
    10321 agttgacccg ttaacaatga aagtccacgg tatggaaaat ttacgtgtag ttgatgcatc
    10381 cgctatgcca cgtacgacaa atggcaatat acacgcacct gttttaatgt tagctgaaaa
    10441 agcagcagac attatacgtg gcagaaagcc tttagagcca caatatattg attattataa
    10501 gcatggtgtt catgatgaaa atgaaggtgc aatcgaagta aaaccatacg ctaaataaat
    10561 ttatatagta gactcttagt ctggcatcta ttgtattggt gccagacttt tttgcgtttt
    10621 atctaaatga gaagttaatg tgttgtttaa aaacggtgtg tgagtaagtg aactgaaaat
    10681 agagttaatt gtacattgtg atgattaatt gacctaattg gcagacatta aaagagtgta
    10741 caaccttaaa aagttcacta aatagacatt taattgtgtt ttcttgaaag ctttcgtggt
    10801 aggatttata taagtaaatt tagagaagga atactatgag cttttacgaa tatatacaaa
    10861 catttaaaga tgataaaaca ccattaggcg aattagcgat ttggattaaa gaagatgatt
    10921 cattcccaaa acaagagaag ctaacagaaa acattttgtc ttattttcat caaatgtcca
    10981 atatagatca tgagttttta gaaattgtaa aaagatcact ttctctgtat gatcaattaa
    11041 aatcgtaaaa tcagaaataa agttagctaa aataaaaggc cggtgatttc acagatgaag
    11101 ttgtcggcta ttttcatgac taatgttaaa taatgatcat gaaattgaag acacaaaaat
    11161 agtgggcgac tcctgtcccc atttgcatga aatattaata ctttttacaa agtcttatct
    11221 tttaaaaaga agccaaacta tgattgtaat taataaatac gggaatgaat tagtcataga
    11281 tataaagtaa tatgaccaaa aatataaatg aaatgaaggc atttattatg aacatttatg
    11341 atgaatatag aagttattta atagaagaac tggatgattg tctcacaata caaaaaaata
    11401 atgacactgc atattacgat gtgctagaag cgataaatga tttatcaaat gattcattat
    11461 gtgtgttaaa tcatttatat attaacgaag gacaagaaga aacattcgag caaaaatttt
    11521 tgcaacgtaa taagcattta aaaaacgttg atgggtttaa agcattaagg tttttaagac
    11581 ctagaacagc tggtagacac tatatcatta ttacgctgtg ggaaaatagg caagcatttt
    11641 atcattggca aaattcagct gagtacaagc atacacacaa gcatcgtggc acttcgaaag
    11701 gtgcagatgt aaaaataatt aatcgtgaat tatcatacaa tattcgtatt gaattagctg
    11761 atatggtcta gcccgagata tcaaccctca atcatttgaa agtccttgaa tgctacgtca
    11821 tggcaaagtg tagtgccgag gactttggta tttaacccta tttaattgaa aattctgtaa
    11881 agataggcgt atgatatata taattcattt ataaaattac aatgttcatc acagggaaag
    11941 tggatgtatg aaaggaagtt agttatgcaa aagtcagctc tattcgcgcc tgaaaaatat
    12001 aacattattt cggaaatcga aaaatatagc cacataccag ataaaaaggc aattttatat
    12061 cataatactg aaggcgaaga tatatcagtc acttaccaac agctcattga acagtctaat
    12121 aaagtaggta atgttttagc atcgcacggt ttatctaaag gcgataaagt actcattatg
    12181 atgccacgat ctattgcgac gtatgaatta tatattgctg ctttaaaatt aggtgttgca
    12241 atcattccat gttctgaaat gctaagaact aaagatttac aataccgtat cacgcacggt
    12301 gaaataaatg cagttattgc actagagcca ttcacagttg aatttgaaaa aattaaagaa
    12361 tatgatgcgt taactaaatt tgttattgcc ggtcaaaaag atggatggat ttcacttgaa
    12421 gctgaaaaag aaaacgcaag taacaaacta gaaatggcag atacgacgcg tgatgatatg
    12481 gcgattctgt catatacatc tggtacaact ggcaatccta aagccgttac acattgtcat
    12541 ggatggggat ttgcacattt acaaatggca ccaaaacatt ggttatgtat taatgaagat
    12601 gacttagttt gggcaacagc agcaccaggt tggcaaaaat gggtatggag tcctttctta
    12661 tctgtattag gttcaggtgc aactgctttt gtatttaatg gaaggttcag tcctgaaaca
    12721 tacttagaat tgcttcaaaa atatcaaatt aatgttttat gttgtacacc gacagaatat
    12781 cgtatgatgg ctaaattaca gaacttaaat gattatgact taacgcattt acatagtgcc
    12841 gtttctgcag gtgagccact aaatcgagaa gtagttgaac aattcaagaa atactttaat
    12901 ttaactgtaa gagatggata tggccagact gaaagtacct tgcttatagg ctttttaaaa
    12961 gatacaccac agaggattgg atcaatgggt aaaggcattc caggtagttc ggtcactgta
    13021 gtagatgatg aggggaatag cgtacctgct aatacgaaag ggaatattgc agtaccactt
    13081 gatttgccag cacttttcaa agggtattat aaagagccag aacgtacggc gaaagcgcaa
    13141 acaggtaaat actacatcac tggtgactta gctcatgtag atgatgatgg ttatttctgg
    13201 tttgaaggac gtcgagatga cattattatt agttcaggct atacgattgg gccttttgaa
    13261 gttgaagatg cgttaacgaa tcatccagcc gttaaagaat gtgctgtagt agcaaaacct
    13321 catgatatac gaggaaatat tgttaaagca ttcgttattt tacaggacca tactgcgggt
    13381 gatgatacgt tagttaaaga actacaacaa tttgttaaaa atgaagttgc accatataaa
    13441 tatccgagag agattgaatt cgttgacgac ttaccaaaaa caaactcagg taaaattcgt
    13501 cgtgttgaac tacgagattc tgaaatagaa aaatggcaac aacaaaaaga tagcaatcaa
    13561 taaacatagc aaagcagtcc aatgcgtaag gtatgatgat acgcactgga ctgcttgttt
    13621 atttatgatg ttctttgtcg tctagttata aatattaaaa ttagtttaga aacaagcgca
    13681 accagtatta agaatgagaa aatataagat gttaaacctc gaatatccca gtttaataaa
    13741 aacacattac tatcaccggc attaagttga aactcagtat gtgttagaac gataattaca
    13801 atacctataa gtgcaagatt caaaataatt ttcaatatta aaggtaattg taatgttttt
    13861 cttggatgca gatattgaat ctccttttta tgattgattt tacacttata ttgtatcaaa
    13921 atagagtata ggtttaaaat gctataagcc aaatgtagat taaaacaatc gttagctatt
    13981 tgttgtcaaa taattatgat tgattt
//
LOCUS       DANBWH010000057        13914 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.059, whole genome shotgun sequence.
ACCESSION   DANBWH010000057 DANBWH010000000
VERSION     DANBWH010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13914
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.059"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            126..1490
                     /gene="radA"
                     /locus_tag="PPY92_001145"
     CDS             126..1490
                     /gene="radA"
                     /locus_tag="PPY92_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDJ2625158.1"
                     /translation="MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPK
                     HGVKTKELAGKVQKLNSIKHETTPRVLTDSAEFNRVLGGGIVSGSLVLIGGDPGIGKS
                     TLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
                     EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEG
                     QIAGPRLLEHMVDTVLYFEGDEHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNP
                     SEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPRRMATGIDHNRLSLL
                     MAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGE
                     VGLTGEVRRVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEALSF
                     ALHS"
     gene            1515..2588
                     /locus_tag="PPY92_001146"
     CDS             1515..2588
                     /locus_tag="PPY92_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017328539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDJ2625159.1"
                     /translation="MNIVKLMVIIIYLIIGSALGIIIIPEIANDLGLQNSSLLKNHYV
                     DGIIGSIFMFLIFGVFIRRVTNAIKGLEHFIMRRSAVEILFATIGLIIGLLISVMVSF
                     ILESIGNSIFNHFIPVIITILLCYFGFQFGLKKRDEMLMFLPENIARSMSQHTKSATP
                     KIIDTSAIIDGRILEVIRCGFIDGNILIPQGVINELQIVADSNDSVKREKGKRGLDIL
                     NELYDLDYPTKVIHPTKTHSDIDTMLLKLAKQYHASIITTDFNLNKVCHVHGIKALNV
                     NDLSEAIKPNVHQGDQLHILLTKMGKEPGQAVGYLDDGTMVVVDNAKNLIGSHVNLEV
                     VSLLQTSSGRIVFAKKIEDTVSL"
     gene            3145..4599
                     /locus_tag="PPY92_001147"
     CDS             3145..4599
                     /locus_tag="PPY92_001147"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDJ2625160.1"
                     /translation="MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIE
                     DTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGYGPYRQSERQHIYQPLIDQLLAE
                     DKAYKCYMTEEELEAEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVP
                     QNQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMQISDVIRGDDH
                     ISNTPKQIMIYEAFGWEPPRFGHMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEA
                     LFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQKLAWVNNQYMKQKDT
                     ETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMP
                     ALGEEEQQVINGEQVPELMTHLFSKLEALEPFEAAEIKKTIKEVQKETGIKGKQLFMP
                     IRVAVTGQMHGPELPNTIEVLGKEKVLNRLKQYK"
     gene            5022..5669
                     /gene="cysE"
                     /locus_tag="PPY92_001148"
     CDS             5022..5669
                     /gene="cysE"
                     /locus_tag="PPY92_001148"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDJ2625161.1"
                     /translation="MILLKRMRDDIKMVFEQDPAARSTLEVITTYAGLHAVWSHLIAH
                     KLYNQKKYVAARAISQISRFFTGIEIHPGAKIGKRLFIDHGMGVVIGETCTIGDNVTI
                     YQGVTLGGTGKERGKRHPDIGDNVLIAAGAKVLGNIKINSNVNIGANSVVLQSVPSYS
                     TVVGIPGHIVKQDGVRVGKTFDHRHLPDPIYEQIKHLERQLEKTRNGEIQDDYII"
     gene            5653..7053
                     /locus_tag="PPY92_001149"
     CDS             5653..7053
                     /locus_tag="PPY92_001149"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDJ2625162.1"
                     /translation="MITLYNTLTRQKEVFKPIEPGKVKMYVCGPTVYNYIHIGNARPA
                     INYDVVRRYFEYQGYNVEYVSNFTDVDDKLIKRSQELNQSVPEIAEKYIAAFHEDVGA
                     LNVRKATSNPRVMDHMDDIIQFIKDLVDQGYAYESGGDVYFRTRKFEGYGKLSHQSID
                     DLKVGARIDAGEHKEDALDFTLWKKAKPGEISWNSPFGEGRPGWHIECSVMAFHELGP
                     TIDIHAGGSDLQFPHHENEIAQSEAHNHAPFANYWMHNGFINIDNEKMSKSLGNFILV
                     HDIIKEVDPDVLRFFMISVHYRSPINYNLELVESARSGLERIRNSYQLIEERAQIATN
                     IENQQTYIDQIDAILNRFETVMNDDFNTANAITAWYDLAKLANKYVLENTTSTEVIDK
                     FKAVYQIFSDVLGVPLKSKNADELLDEDVEKLIEERNEARKNKDFARADEIRDMLKSQ
                     NIILEDTPQGVRFKRG"
     gene            7046..7450
                     /locus_tag="PPY92_001150"
     CDS             7046..7450
                     /locus_tag="PPY92_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDJ2625163.1"
                     /translation="MDNQQDNHIKLLNPLTLAYMGDAVLDQYVRTYIVLKLKSKPNKL
                     HQMSKKYVSAKSQAQTLEYLMEQEWFTDEEMDILKRGRNAKSHTKAKNTDVQTYRKSS
                     AIEAVIGFLYLEKREERLEALLNKIITIVNER"
     gene            7458..8204
                     /gene="rlmB"
                     /locus_tag="PPY92_001151"
     CDS             7458..8204
                     /gene="rlmB"
                     /locus_tag="PPY92_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDJ2625164.1"
                     /translation="MEDTVIVGRHAVREAIITGHPINKILIQEGIKKQQINEILKNAK
                     DQKIIVQTVPKSKLDFLANAPHQGVAALIAPYEYADFDQFLKQQKEKEGLSTVLILDG
                     LEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVIRVTNLAKT
                     IDELKDNGFWVAGTEANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCDFYIKIPM
                     VGHVNSLNASVAASLMMYEVFRKRHDVGEI"
     gene            8204..8728
                     /locus_tag="PPY92_001152"
     CDS             8204..8728
                     /locus_tag="PPY92_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDJ2625165.1"
                     /translation="MKERYLIIDGYNMIGQSPTLSAIAKENLEEARMQLIDAIANYNA
                     VISDEIICVFDAYDQSGVEREYMYHGVKTIFTKEKETADSFIERYVYELYDKHTKHIA
                     VVTSDMSEQHAIFGSGAYRISSREMWRDLKENEIDVSKSLDDISENKPRTRIPLSSEI
                     LAEFEKIRRGHHKK"
     gene            8809..9378
                     /locus_tag="PPY92_001153"
     CDS             8809..9378
                     /locus_tag="PPY92_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDJ2625166.1"
                     /translation="MKYDLTTQDSTIKRNNSINDKDFEKLVMDLQPLIIRRIKTFGFN
                     HYDLEDLYQEILIRMYRSVQTFDFSGEQPFTNYVQCLITSVKYDYLRKYLATNKRMDN
                     LINEYRVTYPCAIKRFDVENNYLNKLAIKELICQFKYLSAFEKDVMYLMCEQYKPREI
                     AQLMHVKEKVIYNAIQRCKNKIKRYFKMI"
     gene            9493..9636
                     /gene="rpmG"
                     /locus_tag="PPY92_001154"
     CDS             9493..9636
                     /gene="rpmG"
                     /locus_tag="PPY92_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002451211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDJ2625167.1"
                     /translation="MRKIPLNCEACGNRNYNVPKQEGSATRLTLKKYCPKCNAHTIHK
                     ESK"
     gene            9692..9874
                     /gene="secE"
                     /locus_tag="PPY92_001155"
     CDS             9692..9874
                     /gene="secE"
                     /locus_tag="PPY92_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDJ2625168.1"
                     /translation="MAKKESFFKGVKSEMEKTSWPTKEELFKYTVIVVSTVIFFLVFF
                     YALDLGITALKNLLFG"
     gene            9887..10435
                     /gene="nusG"
                     /locus_tag="PPY92_001156"
     CDS             9887..10435
                     /gene="nusG"
                     /locus_tag="PPY92_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDJ2625169.1"
                     /translation="MSEEVGAKRWYAVHTYSGYENKVKKNLEKRVESMNMTEQIFRVV
                     IPEEEETQVKDGKAKTTVKKTFPGYVLVELIMTDESWYVVRNTPGVTGFVGSAGAGSK
                     PNPLLPEEVRFILKQMGLKEKTIDVELEVGEQVRIKSGPFANQVGEVQEIETDKFKLT
                     VLVDMFGRETPVEVEFDQIEKL"
     gene            10616..11038
                     /gene="rplK"
                     /locus_tag="PPY92_001157"
     CDS             10616..11038
                     /gene="rplK"
                     /locus_tag="PPY92_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012664221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDJ2625170.1"
                     /translation="MAKKVDKVVKLQIPAGKANPAPPVGPALGQAGVNIMGFCKEFNA
                     RTQDQAGLIIPVEISVYEDRSFTFITKTPPAPVLLKKAAGIEKGSGEPNKTKVATVTK
                     DQVREIANSKMQDLNAADEEAAMRIIEGTARSMGIVVE"
     gene            11246..11938
                     /gene="rplA"
                     /locus_tag="PPY92_001158"
     CDS             11246..11938
                     /gene="rplA"
                     /locus_tag="PPY92_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDJ2625171.1"
                     /translation="MAKKGKKYQEAASKVDRTQHYSVEEAIKLAKETSIANFDASVEV
                     AFRLGIDTRKNDQQIRGAVVLPNGTGKSQSVLVFAKGDKIAEAEAAGADYVGEAEYVQ
                     KIQQGWFDFDVVVATPDMMGEVGKLGRVLGPKGLMPNPKTGTVTMDVKKAVEEIKAGK
                     VEYRAEKAGIVHASIGKVSFTDEQLIENFNTLQDVLAKAKPSSAKGTYFKSVAVTTTM
                     GPGVKIDTASFK"
     misc_feature    12026..12172
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            12210..12710
                     /locus_tag="PPY92_001159"
     CDS             12210..12710
                     /locus_tag="PPY92_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDJ2625172.1"
                     /translation="MSAIIEAKKQLVDEIAEVLSNSVSTVIVDYRGLTVAEVTDLRSQ
                     LREAGVEYKVYKNTMVRRAAEKAGIEGLDEFLTGPTAIATSSEDAVAAAKVISGFAKD
                     HEALEIKSGVMEGNVITAEEVKTVGSLPSHDGLVSMLLSVLQAPVRNFAYAVKAIGEQ
                     KEENAE"
     gene            12753..13121
                     /gene="rplL"
                     /locus_tag="PPY92_001160"
     CDS             12753..13121
                     /gene="rplL"
                     /locus_tag="PPY92_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDJ2625173.1"
                     /translation="MANHEQIIEAIKEMSVLELNDLVKAIEEEFGVTAAAPVAVAGAA
                     GGADAAAEKTEFDVELTSAGSSKIKVVKAVKEATGLGLKDAKELVDGAPKVIKEALPK
                     EEAEKLKEQLEEVGATVELK"
     gene            13296..13904
                     /locus_tag="PPY92_001161"
     CDS             13296..13904
                     /locus_tag="PPY92_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDJ2625174.1"
                     /translation="MSHYYDEDPSVISNEQRIQYQLNHHKIDLITDNGVFSKDKVDYG
                     SDVLVQTFLKAHPPGPSKRIADVGCGYGPIGLMIAKVSPHHSITMLDVNHRALALVEK
                     NKKLNGIDNVIVKESDALSAVEDKSFDFILTNPPIRAGKETVHRIFEQALHRLDSNGE
                     LFVVIQKKQGMPSAKKRMNELFGNVEVVNKDKGYYILRSIKA"
BASE COUNT         5151 a         1882 c         2734 g         4147 t
ORIGIN      
        1 tctggtaaaa cagtttgcgt tagtagtcat gttaaaatac aaacaaatgt aactcacatt
       61 taatttgtca taatgggaat gtgcgtttaa aatagatttg ttcaaaggaa agtggaggtg
      121 caattttggc caagaaaaaa gtgatttttg aatgtatggc ttgtggttat caatctccta
      181 aatggatggg gaaatgtcct aattgtggcg cttggaatca aatggaggaa attgttgaaa
      241 aagcagccaa tcctaaacat ggagttaaaa ccaaggaatt agcaggtaaa gtacaaaaat
      301 taaatagtat taaacatgaa acaacgccga gagtgttaac agattcagca gaattcaacc
      361 gtgtattagg tggaggtatt gtgagcggat cgttagtact tattggtggg gatccaggta
      421 ttggtaagtc aacgttactt ttacaaattt gtgcatcgtt atctcaaaag aaaaaagtac
      481 tatatattac tggagaagaa tcgcttagtc agactaaatt acgtgcagag cgattagatg
      541 aagattcaag tgaattgcaa gtattagctg aaacagatct tgaagttatt tatcaaacag
      601 taaaagaaga acaacctgat ttattagtag tggattcgat tcaaacaata tatcatcctg
      661 aaatcagctc tgcgccaggt tctgtttcac aagttcgtga aagtacacaa agtttaatga
      721 atattgctaa acaaatgaac attgcaactt ttatagtggg tcatgtaacg aaagaaggtc
      781 aaattgctgg cccaagattg ctagaacaca tggttgatac tgtgctttat tttgaaggcg
      841 atgaacacca cgcatatcga attttgcgag ctgttaaaaa ccgttttggt tcaacgaatg
      901 aaatgggaat cttcgaaatg aagcaaagtg gattaaaagg tgtaaataat ccatctgaaa
      961 tgtttttaga agaacgttca acaaatgttc caggttcaac aattgttgca accatggagg
     1021 gaaccagacc acttttaata gaagttcaag cgctggtaac tccaacgact tttaacaatc
     1081 cgagacgaat ggcaacaggg attgatcata atcgattaag tttgttgatg gctgttttgg
     1141 aaaagaaaga aaattatcta ttacaacaac aagatgctta tatcaaagta gctggcggtg
     1201 taaagttaac ggagccagca gttgatttaa gtgtaattgt agcaactgca tctagcttta
     1261 aagataaagc tgtcgacgga ttagattgct atattggaga agttggttta acgggtgagg
     1321 tacgtcgtgt atctcggata gaacaacgcg tgcaagaggc tgcaaaacta ggtttcaaac
     1381 gtgtaattat tcctaaaaat aatataggcg gatggacata tcctgaaggt atacaagtaa
     1441 taggtgtaac tactgtacat gaagcattgt catttgctct tcattcataa aacatcaaga
     1501 aaggaggaca ttgtgtgaat atcgttaaac taatggttat tattatttac ttaattattg
     1561 ggagcgcatt aggaataatt attattcctg aaattgcaaa tgatcttgga ttacaaaact
     1621 ccagcttatt aaaaaatcac tatgtagatg gcattatcgg tagtattttt atgttcttaa
     1681 tttttggtgt atttattaga cgagttacta acgctataaa aggtttagaa cattttatta
     1741 tgcgtagaag tgctgttgaa atactattcg caacaatagg tttaataatc ggattactta
     1801 tttctgttat ggtgtcgttt atattagaat caattggtaa ctctattttt aatcatttca
     1861 ttcctgtcat aattacgata ttactatgtt atttcggttt ccaatttggc cttaaaaaac
     1921 gagatgaaat gttaatgttt ttacctgaga atatagcgcg ttccatgtca caacatacta
     1981 aaagtgctac gccaaaaatt atcgacacaa gcgcaattat tgatggtcgt attttagaag
     2041 tcattcgttg cggttttatc gatggcaata ttttaattcc gcaaggtgtt attaatgaat
     2101 tacaaattgt tgcagattca aatgacagtg ttaaacgtga aaagggtaaa agaggcttag
     2161 atattttaaa tgaattgtat gatttagatt atcctacaaa ggttatacat ccaactaaaa
     2221 cacatagtga tattgataca atgctattaa agctagctaa acaatatcat gcaagtatta
     2281 ttacgacaga tttcaaccta aataaagttt gtcatgtaca tggtatcaaa gcattaaatg
     2341 ttaatgattt atcagaagca atcaaaccta atgtacatca aggtgatcaa ctgcatattt
     2401 tactgacaaa aatgggtaaa gagcctggtc aggcagtagg atatctagat gatggtacga
     2461 tggtagttgt tgataatgct aaaaatctta ttggcagtca tgtcaattta gaagtagtca
     2521 gcttattgca aacatcttca ggaagaattg tttttgctaa aaaaatcgaa gatacagtat
     2581 cattataaaa actgtagttg acgaaaacaa aatagatatt aaaaggtata tgaaatggcg
     2641 aagtttgtaa tttgaagtat aactataaat aaatgcaaaa catatcagtt atcaaaaaat
     2701 acaaatagtg ctaatatatc attgaaacaa gttcattaag tgttaaattt tagatattaa
     2761 taaagttatt cttatagcaa tcaagacata caaaattaga atcaatttta acgtatttga
     2821 atagttaatt tgaagtttaa ttttacagta cataatattc atttttaagt cgttgaaatg
     2881 cttatgtagt gcagtcttga ttcttaggga tagctttttt aaagtgttga aaaaacaact
     2941 gctttctatt taaaatttaa gcatattacc cagtgaatta tgttatgatt aaacaatgaa
     3001 attattttaa ggtcatgtta aaaagcattg tttcgtttta acaataagac gagttagaca
     3061 tttgttatga cggtcgaaga tagcaaatga ataatttata tgaactaact tgaaaatctg
     3121 atatttaatt taggagtgaa aaaaatgagc gatcgtataa gagtaagata tgcaccaagt
     3181 ccaactggtt atcttcatat tggtaatgca agaacagcat tattcaatta cttgtatgct
     3241 aaacattaca acggagattt tgtgattcga attgaagata ctgataaaaa acgtaattta
     3301 gaagatggag aaacatcaca atttgataat cttaaatggt taggattaga ttgggatgag
     3361 tctgtagata aagacaatgg ctacggacca tatcgtcaat ctgaacgtca acatatctac
     3421 caaccattaa tagatcagtt actagcagaa gataaagcat ataaatgcta tatgacagaa
     3481 gaagaattag aagctgaacg tgaagcgcaa atcgctcgtg gtgaaatgcc tcgctatggt
     3541 ggtcaacatg cgcatttgac tgaagaacaa cgtcaacaat ttgaagcaga aggacgccaa
     3601 ccatcaattc gtttccgagt acctcaaaac caaacgtatt catttgatga tatggtaaaa
     3661 ggaaatattt catttgattc aaatggtatt ggtgactggg ttatcgtaaa aaaagatggc
     3721 attccaacgt acaattttgc agtagctata gatgatcatt acatgcaaat ttcagatgta
     3781 attcgtggtg atgatcatat ttcaaacacg cctaaacaaa ttatgattta tgaagcattt
     3841 ggctgggagc cacctcgttt tggtcatatg tcattaattg ttaatgaaga acgtaaaaag
     3901 ttaagtaaac gtgatgggca aattttacaa tttattgagc aatatcgtga cttaggttat
     3961 ttacctgaag cgttatttaa ttttattgcg ttattaggtt ggtctcctga aggtgaagaa
     4021 gaaatctttt ctaaagaaga atttatcaaa atctttgatg aaaagcgttt gtcaaaatca
     4081 ccagcatttt tcgataagca aaaattagca tgggttaata accaatatat gaaacaaaaa
     4141 gatactgaaa cagtattcca attagcatta cctcatttaa ttaaagcaaa tttgattcct
     4201 gaggtgccgt cagaagagga tttatcttgg ggacgcaaat taattgcgct ttatcaaaaa
     4261 gaaatgagtt atgccggtga aattgtacct ttatcagaaa tgttctttaa agaaatgcca
     4321 gctcttggtg aagaagaaca acaagtgatt aatggagagc aagtaccaga gttaatgacg
     4381 cacttattca gtaaattaga agcacttgaa ccatttgaag cggctgaaat taaaaagaca
     4441 attaaagaag ttcaaaaaga aacaggaata aaaggcaagc aattatttat gcctattcgt
     4501 gttgctgtaa caggccaaat gcatggtcct gaattaccaa atacaattga agtacttggt
     4561 aaagaaaaag tgctaaaccg tttaaaacaa tataagtaat gaaatagaca agatcaaggt
     4621 agtatatact tgaattttat aacgtaacca catatgataa agttgtatta acaaattaca
     4681 acaatatgaa taaagtattt tatataaaac gaaggattag taattaaatt tatacgatgc
     4741 agagagtgta cggttgctgt gagtacaacg tagaaattaa tgaatgcacc ttcgtaaaat
     4801 gaattaaata tataatgaga gtgatgagca ttaagttgac ttagtttcct tgataatttg
     4861 gaagcgcccg caatattatt aatgttattc gctaaattca gagtggaacc gtgcggaagc
     4921 gcctctaaca atacaatttg tatgttagtg gtgctttttt gatatttaat ttcgcaggta
     4981 cttcaattat gtatttacgt agttcaatca ttgaggagga aatgatcttg ttaaaaagaa
     5041 tgagagacga tataaaaatg gtatttgagc aggatccagc ggcacgttca acattagaag
     5101 tcattacaac gtatgcaggt ttacatgcag tttggagtca tttgattgca cataagttat
     5161 acaaccaaaa aaaatatgtt gcagcacgcg cgatatctca aatttcaaga tttttcacag
     5221 gtatagaaat ccatccaggt gctaaaattg gaaagcgtct atttatagat catggtatgg
     5281 gcgttgtaat aggagaaaca tgtacaattg gtgataatgt gacaatctat caaggcgtga
     5341 cacttggtgg gacagggaaa gaaagaggga aaagacaccc agatatagga gacaatgttt
     5401 taatagcagc cggtgcgaaa gttttaggaa atattaaaat aaattcaaat gtaaatattg
     5461 gtgcaaattc agttgtttta caatcagttc caagttattc aacggttgtt ggtataccag
     5521 gacatattgt taagcaagat ggtgttcgag ttggaaaaac atttgatcat cgccatctac
     5581 ctgatccaat ttatgaacaa attaagcatt tagaacgaca acttgaaaag actaggaatg
     5641 gagagattca agatgattac attatataat acgcttacac gtcaaaaaga agtgttcaag
     5701 cctatagaac cagggaaagt aaaaatgtat gtatgtggtc ctactgtata taactacatt
     5761 catattggta acgcaagacc agcaattaat tatgacgtag tgagacgtta ctttgaatac
     5821 caaggatata atgtagaata tgtatcaaat tttacagacg tagatgataa attaattaaa
     5881 cgttctcaag aattaaatca gtctgttccc gaaattgcag aaaaatatat cgctgctttt
     5941 catgaagatg ttggtgcgtt aaatgttaga aaagcgactt caaatccaag ggtaatggac
     6001 catatggatg acattattca atttattaaa gatttggtgg atcaaggtta tgcatatgaa
     6061 agtggtggcg atgtttactt tagaacacgt aaatttgaag gttatggtaa attaagtcat
     6121 caatccatag atgacttaaa agtgggtgct cgtatagatg caggagagca taaagaagat
     6181 gcacttgatt ttacattgtg gaaaaaagcg aagcctggcg agattagttg gaatagccca
     6241 tttggtgaag gtagaccagg atggcatata gaatgttctg taatggcatt tcatgagcta
     6301 ggacctacaa ttgatataca tgcgggtggt tcagatttac aatttccaca tcatgaaaat
     6361 gaaatagcac aatcagaagc acataatcat gcgccatttg ctaattattg gatgcataat
     6421 ggtttcatta atattgataa tgaaaaaatg agtaaatcac taggcaactt tattttagtt
     6481 cacgatatta ttaaagaagt tgatccagat gtactaagat tctttatgat tagcgtacat
     6541 tatagaagcc caattaacta taatctagaa ttggtagaat cagcacgtag tggactagag
     6601 cgtattcgca atagttatca attaattgaa gagcgcgcac aaattgctac taatattgaa
     6661 aatcaacaga catatattga tcaaattgat gcgattttaa atcgttttga aacagttatg
     6721 aatgatgatt ttaatacagc taatgcaatt acagcttggt atgatttagc aaaacttgcg
     6781 aataaatatg tactagagaa cacaacatca acagaagtaa ttgataaatt taaagcagtt
     6841 tatcaaattt tcagcgatgt tttaggtgta ccgttaaaat ctaaaaatgc agatgaatta
     6901 ttggatgaag atgttgaaaa attaatcgaa gagcgtaatg aagcaaggaa aaacaaagat
     6961 tttgcacgag cagatgagat tcgagacatg ctgaaatcac aaaacattat attagaagac
     7021 acacctcaag gggttagatt taaacgtgga taatcaacaa gataatcaca ttaaattatt
     7081 gaatccattg accttagcat atatgggaga cgcagtctta gatcaatatg tacgtaccta
     7141 tatcgtttta aagcttaaaa gtaagcctaa taaactacat caaatgtcta aaaaatatgt
     7201 atctgccaaa agtcaggcgc aaacgttaga atatttaatg gagcaagaat ggtttacaga
     7261 cgaagaaatg gatattttga agcgagggcg taacgcgaaa agtcatacta aagctaaaaa
     7321 cactgatgtt caaacatatc gtaaaagttc agcgatagaa gcagtgatag gttttcttta
     7381 tttagaaaaa agagaagaac gattagaggc attattaaat aaaataataa caatagtaaa
     7441 cgaaaggtag tgacgatgtg gaagatacgg ttattgttgg taggcatgct gttagagaag
     7501 cgattattac tgggcatccg ataaataaga tattgattca agaaggtatt aaaaagcaac
     7561 aaattaatga aattttaaaa aatgcaaaag atcaaaaaat tattgttcaa actgtaccaa
     7621 aatctaaatt agatttttta gcaaatgcac cacatcaggg tgttgcagcg cttattgcac
     7681 catatgaata tgctgacttc gatcaatttt taaaacagca aaaagaaaaa gaaggtttat
     7741 cgacagtact tatattagac ggcttagaag acccgcataa cttgggatca attttaagaa
     7801 cagccgatgc aacgggagtt gatggtgtta ttattcctaa acgtcgttca gttacactaa
     7861 cgcaaacagt tgcaaaagcc tcaacaggtg caattgaaca tgtaccagtt attcgagtga
     7921 caaatttagc taaaactatc gatgaactaa aagataatgg cttttgggta gctggcactg
     7981 aagctaataa tgcaacagat tatagaaatc tagaagcgga catgtcattg gctattgtaa
     8041 ttggtagcga aggacagggt atgagtcgcc tagtaagtga taaatgcgat ttttatatta
     8101 agattccaat ggttggacat gtaaacagtt tgaatgcttc ggttgcagca agtttaatga
     8161 tgtacgaagt atttcgaaaa agacatgatg ttggagaaat ataatgaaag aacgttactt
     8221 aatcattgat ggatacaata tgataggaca atcaccaacg ctaagcgcca ttgcaaaaga
     8281 gaatttagaa gaagctagaa tgcaattaat agatgcaatt gcaaattata atgcagttat
     8341 ttcagatgaa attatttgtg ttttcgatgc ttatgaccaa tcgggtgttg aaagagaata
     8401 catgtatcat ggcgttaaaa cgatttttac caaggaaaaa gaaacagctg atagtttcat
     8461 agaacgttat gtttatgaac tttatgacaa gcatactaag catattgcag ttgtaacaag
     8521 tgatatgagt gagcaacatg ctatctttgg atcaggtgca tatagaatat catctcgcga
     8581 aatgtggaga gatttaaaag aaaatgaaat tgatgtgagt aaatcattag atgatataag
     8641 tgaaaacaag ccaagaactc gaattccgtt atcttctgaa atccttgcag aatttgaaaa
     8701 aatacgaaga ggacatcata agaaatgaca tttccgtcat cttgaaattt tgaatgtaac
     8761 aatattaaac taaccttaaa ttttagatag aaggggttag tttaatactt gaaatatgat
     8821 ttgacaactc aagacagtac aatcaaacgt aacaattcaa taaatgataa agacttcgaa
     8881 aagttagtaa tggatctaca accattaatt attcgacgca tcaaaacatt tggatttaat
     8941 cattatgatt tagaagactt atatcaagaa atacttatac ggatgtatag gtcggtccaa
     9001 acatttgatt ttagtggaga gcagcctttc acaaattatg ttcaatgttt aattacgtct
     9061 gtaaagtatg attatttgag aaaatattta gctacaaata aaagaatgga taatttgatt
     9121 aatgaatata gagttacgta tccatgtgca ataaagcgtt ttgatgttga aaacaattat
     9181 ttgaataaat tagcaattaa agagttgatt tgtcagttta agtatttgag tgcatttgaa
     9241 aaagatgtca tgtatttaat gtgtgaacaa tataagccga gagaaattgc tcaattgatg
     9301 catgtaaaag agaaagtgat ttataatgcc atacaacgat gtaaaaataa aataaaacgt
     9361 tatttcaaaa tgatttgaaa agcgccttag gacgtgaatt gaattataac gtgttactta
     9421 ctgatggttt gacatttgtt ataaatttta tgtatagtat actggtatta taatgaataa
     9481 aggtgaatta ttgtgagaaa aataccttta aattgtgaag cttgtggcaa tagaaattat
     9541 aatgttccta agcaagaagg ctcggcaaca agattaacct taaagaaata ttgtccaaaa
     9601 tgtaacgcgc acacaattca taaagaatcg aaataaatac attcgaaata atactttgat
     9661 aatatgttca aaggatttgg aggttgagca gatggctaaa aaagaaagtt tctttaaagg
     9721 cgttaagtct gaaatggaaa aaacaagttg gccgacgaaa gaagagctat ttaaatatac
     9781 tgtaattgta gtttctactg ttatattctt cttagtcttt ttctatgcct tagatttagg
     9841 aattacagca ttgaaaaatt tattatttgg ttagaggagt gaagacatgt ctgaagaagt
     9901 tggcgcaaag cgttggtatg cagtgcatac atattctgga tatgaaaata aagttaaaaa
     9961 gaatttagaa aaaagagtag aatctatgaa tatgactgaa caaatcttta gagtagtcat
    10021 accggaagaa gaagaaactc aagtaaaaga tggcaaagct aaaacgactg ttaaaaaaac
    10081 attccctgga tatgttttag tggaattaat catgacagat gaatcatggt atgtggtaag
    10141 aaatacacca ggcgttactg gttttgtagg ttctgcaggt gcagggtcta agccaaatcc
    10201 attgttacca gaagaagttc gcttcatctt aaaacaaatg ggtcttaaag aaaagactat
    10261 cgatgttgaa ctcgaagttg gcgagcaagt tcgtattaaa tcaggtccat ttgcgaatca
    10321 agttggtgaa gttcaagaaa ttgaaacaga taagtttaag ctaacagtat tagtagatat
    10381 gtttggccga gaaacaccag tagaagttga attcgatcaa atagaaaagc tttaattaac
    10441 aattaaagtt attaaactaa ccaaaagata aaaaagagta ttgatttttt aattagaaaa
    10501 gtgttaaaat tatgtggtcg cgcttttaga gcgcccattt cgtcacgaaa tgttaagagt
    10561 gggagggcaa aactgagccc tgtgaccaca tcacgatatc aaggaggtgc acatcgtggc
    10621 taaaaaagta gataaagttg ttaaattaca aattcctgca ggtaaagcga atccagcacc
    10681 accagttggt ccagcattag gtcaagcagg tgtgaacatc atgggattct gtaaagagtt
    10741 caatgcacgt actcaagatc aagcaggttt aattattccg gtagaaatca gtgtttatga
    10801 agatcgttca tttacattta ttacaaaaac tccaccggct ccagtattac ttaaaaaagc
    10861 agctggtatt gaaaaaggtt caggcgaacc aaacaaaact aaagttgcta cagtaactaa
    10921 agatcaagta cgcgaaattg ctaacagcaa aatgcaagac ttaaacgctg ctgacgaaga
    10981 agcagctatg cgtattatcg aaggtactgc acgtagtatg ggtatcgttg tagaataatt
    11041 ttacgaatat taaatttgat tacatgattt aaacgatgaa gcagataaca gagataataa
    11101 tgatgaatta taaatataat ctgaatgact agattaatga ttgatttatt cataagatta
    11161 attcttctgt tgtctgctct taacttgcat atagcaagta atgtgggagg aaattccgct
    11221 aaaaccacta aaggaggaac tataaatggc taaaaaaggt aaaaagtatc aagaagcagc
    11281 tagtaaagtt gaccgtactc agcactacag tgttgaagaa gcaattaaat tagctaaaga
    11341 aacaagcatt gctaactttg acgcttctgt tgaagttgca ttccgtttag gaattgatac
    11401 acgtaaaaat gaccaacaaa tccgtggtgc agttgtatta ccaaacggaa ctggtaaatc
    11461 acaaagtgta ttagtattcg ctaaaggtga caaaattgct gaagctgaag cagcaggtgc
    11521 tgactatgta ggtgaagcag aatacgttca aaaaatccaa caaggttggt tcgacttcga
    11581 tgtagtagtt gctacaccag acatgatggg tgaagttggt aaattaggtc gtgtattagg
    11641 accaaaaggt ttaatgccaa accctaaaac tggaactgta acaatggatg ttaaaaaagc
    11701 tgttgaagaa atcaaagctg gtaaagtaga atatcgtgct gaaaaagctg gtatcgtaca
    11761 tgcatcaatt ggtaaagttt catttactga tgaacaatta attgaaaact tcaatacttt
    11821 acaagatgta ttagctaaag ctaaaccatc atctgctaaa ggtacatact tcaaatctgt
    11881 tgctgtaact acaacaatgg gtcctggagt taaaattgat actgcaagtt tcaaataata
    11941 aatgatataa acaattacag gctgaaagaa atatctttca gtctgtaaaa atatattgac
    12001 aataagtaat ttccaagtta tattacttat tgtgattatt ttacctaaga cagtaggagt
    12061 tatttataac ttaaaattta tcctgccgag gctaaaattg acttgaacgt gatgatctat
    12121 gatctttcaa gcactttttg ccgtgggtag aaagtgcttt ttttattaat tttaaaaaaa
    12181 gcaccaaaaa tttaaatgga ggtgtctgaa tgtctgctat cattgaagct aaaaaacaac
    12241 tagttgatga aattgctgag gtactatcaa attcagtttc aacagtaatc gttgactatc
    12301 gtggattaac agtagctgaa gttactgact tacgttcaca attacgtgaa gctggtgttg
    12361 agtataaagt atacaaaaac actatggtac gtcgtgcagc tgaaaaagct ggtatcgaag
    12421 gcttagatga attcttaaca ggtcctactg ctattgcaac ttcaagtgaa gatgctgtag
    12481 ctgcagcgaa agtaatttct ggatttgcta aagatcatga agcattagaa attaaatcag
    12541 gtgttatgga aggcaatgtt attacagcag aagaagttaa aactgttggt tcattacctt
    12601 cacacgatgg tcttgtatct atgcttttat cagtattaca agctcctgta cgcaacttcg
    12661 cttatgcggt taaagctatt ggagaacaaa aagaagaaaa cgctgaataa tttttagcgt
    12721 aaaaaaatta aaaataatgg aggaattata aaatggctaa tcatgaacaa atcattgaag
    12781 cgattaaaga aatgtcagta ttagaattaa acgacttagt aaaagcaatt gaagaagaat
    12841 ttggtgtaac tgcagctgct ccagtagcag tagcaggtgc agctggtggc gctgacgctg
    12901 cagcagaaaa aactgaattt gacgttgagt taacttcagc tggttcatct aaaatcaaag
    12961 ttgttaaagc tgttaaagaa gcaactggtt taggattaaa agatgctaaa gaattagtag
    13021 acggagctcc taaagtaatc aaagaagctt tacctaaaga agaagctgaa aaacttaaag
    13081 aacaattaga agaagttgga gctactgtag aattaaaata attcaagtat cttaaactta
    13141 ataatcaaag ttttatagca agtattgcta taatataatg attctttgag aagttaaaac
    13201 cccgttattt tgataacggg gttttattca tttaaagact gagtgaaatg ttataattat
    13261 aatgacgagt tacaaagtga agatgaggtg gaataatgag tcattattac gatgaagatc
    13321 caagtgtaat tagcaatgaa caacgtattc aatatcaatt aaaccatcat aaaattgatt
    13381 taataactga taacggagtg ttttcgaaag ataaagtaga ttatggttca gatgttcttg
    13441 ttcaaacttt tttaaaagcg catccacctg gtccaagtaa gcgaattgcc gatgttggtt
    13501 gtggttacgg accaattggt ttgatgattg ctaaagtatc accacatcat tcaattacaa
    13561 tgctagatgt taatcacaga gcgctagcct tagttgaaaa aaacaaaaaa ttaaatggta
    13621 ttgataatgt gatcgtaaag gaaagtgatg ctttgtctgc tgtggaagac aaaagttttg
    13681 attttatttt aaccaatcca ccaataagag cagggaaaga aaccgtgcat cgtatattcg
    13741 agcaagcatt acatagatta gactcgaacg gtgaactatt cgttgtaatt cagaagaagc
    13801 aaggtatgcc atctgcaaag aaaagaatga atgaactttt tggaaatgta gaagtggtaa
    13861 ataaagataa aggatattac attctgagaa gtataaaagc ttgaaatgaa atgg
//
LOCUS       DANBWH010000058        13549 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.060, whole genome shotgun sequence.
ACCESSION   DANBWH010000058 DANBWH010000000
VERSION     DANBWH010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13549
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.060"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..217
                     /locus_tag="PPY92_001162"
     CDS             <1..217
                     /locus_tag="PPY92_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HDJ2625175.1"
                     /translation="PEFFNYVFNDIGEDERQHSIIVGDSLTSDILGGINAGIATCWFN
                     FRGFDHNPGIIPDYEINSWKQLNDIVR"
     gene            complement(326..766)
                     /locus_tag="PPY92_001163"
     CDS             complement(326..766)
                     /locus_tag="PPY92_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDJ2625176.1"
                     /translation="MSEIKRLEINYKTDELFENFRAFGNKDLYMVNELNGQMIDASSD
                     SPFYGIFVGDQLGARMALLKKGDVEEIYFPDFEDYILLWKLEVLPKYQNRGYASELID
                     FAKSFNMPIKAIGRNDSKDFFLHHGFTDVEAKNIEGHDVLLWKP"
     gene            961..2574
                     /gene="bshC"
                     /locus_tag="PPY92_001164"
     CDS             961..2574
                     /gene="bshC"
                     /locus_tag="PPY92_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HDJ2625177.1"
                     /translation="MDCKVVSLNEKDQFIPKIKSSDPVITGLFQYDAAQQTSFEKRMS
                     KENNGREAALANVIREYMSDLKLSNEQELNIQHLANGSKVVIGGQQAGLFGGPLYTFH
                     KIFSIITLSKELTDTHKQQVVPVFWIAGEDHDFDEVNHTFVYNENHGSLHKVKYHTME
                     MPETTVSRYYPDKAELKQTLKTMFIHMKETVHTQGLLEICDRIIDQYDSWTDMFKALL
                     HETFKAYGVLFIDAQFEPLRKMEAPMFKKILKKHQLLDDAFRATQQRTQNQGLNAMIQ
                     TDTNVHLFLHDENMRQLVSYDGKHFKLNKTDKTYIKEEIINIAENQPELFSNNVVTRP
                     LMEEWLFNTVAFVGGPSEIKYWAELKDVFELFDVEMPIVMPRLRITYLNDRIEKLLSK
                     YNIPLEKVLVDGVEGERSKFIREQASHQFIEKVEGMIEQQRRLNKDLLDEVAGNQNNI
                     NLVNKNNEIHIQQYDYLLKRYLLNIERENDISMKQFREIQETLHPMGGLQERIWNPLQ
                     ILNDFGTDVFKPSTYPPLSYTFDHIIIKP"
     gene            2718..3149
                     /gene="mraZ"
                     /locus_tag="PPY92_001165"
     CDS             2718..3149
                     /gene="mraZ"
                     /locus_tag="PPY92_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDJ2625178.1"
                     /translation="MFMGEYDHQLDTKGRMIIPSKFRYDLNERFIITRGLDKCLFGYT
                     LDEWQQIEEKMKTLPMTKKDARKFMRMFFSGAVEVELDKQGRINIPQNLRKYANLTKE
                     CTVIGVSNRIEIWDRETWNDFYEESEESFEDIAEDLIDFDF"
     gene            3165..4100
                     /gene="rsmH"
                     /locus_tag="PPY92_001166"
     CDS             3165..4100
                     /gene="rsmH"
                     /locus_tag="PPY92_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDJ2625179.1"
                     /translation="MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLND
                     DGRLIAIDQDQTAIDNAKEVLKDHLHKVTFVHSNFRELTQILKDLNIEKVDGIYYDLG
                     VSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFS
                     KQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELS
                     AFEDSIEQAIELVKVDGRISVITFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTP
                     KLKRVNRKPITATEEDLDDNNRARSAKLRVAEILK"
     gene            4114..4515
                     /gene="ftsL"
                     /locus_tag="PPY92_001167"
     CDS             4114..4515
                     /gene="ftsL"
                     /locus_tag="PPY92_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDJ2625180.1"
                     /translation="MAVEKVYQPYDEQVYNSIPKQQPQTKPEKKTVSRKVVVQLTKFE
                     KVLYITLITVIAMLSIYMLSLKMDAYDTRGKIADLDYKIDKQSSENSALQSEIKKNSS
                     YERIYEKAKKQGMSLENDNVKVVRSNGEAKN"
     gene            4496..6730
                     /locus_tag="PPY92_001168"
     CDS             4496..6730
                     /locus_tag="PPY92_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDJ2625181.1"
                     /translation="MAKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNG
                     QDLVMKANEKYLVKNAQQPERGKIYDRNGKVLAEDVERYKLVAVIDKKASANSKKPRH
                     VVDKKETAKKLSTVIDMKPEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPG
                     ISLLPETERFYPNGNFASHLIGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIH
                     DIWGYIAPNTKKEKQPKRGDDVHLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAK
                     TGEILAYSQRPTFNPETGKDFGKKWANDLYQNTYEPGSTFKSYGLAAAIQEGAFDPDK
                     KYKSGHRDIMGSRISDWNRVGWGEIPMSLGFTYSSNTLMMHLQDLVGADKMKSWYERF
                     GFGKSTKGMFDGEAPGQIGWSNELQQKTSSFGQSTTVTPVQMLQAQSAFFNDGNMLKP
                     WFVNSVENPVSKRQFYKGQKQIAGKPITKDTAEKVEKQLDLVVNSKKSHAANYRIDGY
                     EVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMGDAPKKNPKVIVYAGMSLAQKNDQEAY
                     ELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYSKVPDVEGQDKQKAIDNVSAKSLEP
                     VTIGSGTQIKAQSIKAGNKVLPHSKVLLLTDGDLTMPDMSGWTKEDVIAFENLTNIKV
                     NLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNSTNNSDSNSDDKKKSDSKTDK
                     DKSD"
     gene            7022..7987
                     /locus_tag="PPY92_001169"
     CDS             7022..7987
                     /locus_tag="PPY92_001169"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDJ2625182.1"
                     /translation="MIFVYALLALVITFVLVPVLIPTLKRMKFGQSIREEGPQSHMKK
                     TGTPTMGGLTFLLSIVITSLVAIIFVDQANPIILLLFVTIGFGLIGFIDDYIIVVKKN
                     NQGLTSKQKFLAQIGIAIIFFVLSNVFHLVNFSTSIHIPFTNVAIPLSFAYVIFIVFW
                     QVGFSNAVNLTDGLDGLATGLSIIGFTMYAIMSFVLGETAIGIFCIIMLFALLGFLPY
                     NINPAKVFMGDTGSLALGGIFATISIMLNQELSLIFIGLVFVIETLSVMLQVASFKLT
                     GKRIFKMSPIHHHFELIGWSEWKVVTVFWAVGLISGLIGLWIGVH"
     gene            7989..9338
                     /locus_tag="PPY92_001170"
     CDS             7989..9338
                     /locus_tag="PPY92_001170"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDJ2625183.1"
                     /translation="MLNYTGLENKNVLVVGLAKSGYEAAKLLSKLGANVTVNDGKDLS
                     QDAHAKDLESMGISVVSGSHPLTLLDNNPIIVKNPGIPYTVSIIDEAVKRGLKILTEV
                     ELSYLISEAPIIAVTGTNGKTTVTSLIGDMFKKSRLTGRLSGNIGYVASKVAQEVKPT
                     DYLVTELSSFQLLGIEKYKPHIAIITNIYSAHLDYHENLENYQNAKKQIYKNQTEEDY
                     LICNYHQRQVIESEELKAKTLYFSTQQEVDGIYIKDGFIVYKGVRIINTEDLVLPGEH
                     NLENILAAVLACILAGVPIKAIIDSLTTFSGIEHRLQYVGTNRTNKYYNDSKATNTLA
                     TQFALNSFNQPIIWLCGGLDRGNEFDELIPYMENVRAMVVFGQTKAKFAKLGNSQGKS
                     VIEANNVEDAVDKVQDIIEPNDVVLLSPACASWDQYSTFEERGEKFIERFRAHLPSY"
     gene            9354..10673
                     /locus_tag="PPY92_001171"
     CDS             9354..10673
                     /locus_tag="PPY92_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDJ2625184.1"
                     /translation="MDDKTKNDQQESNEDKDELELFTRNTSKKRRQRKRSKATHFSNQ
                     NKDDTSQQADFDEEIYLINKDFKKEESNDENNGSASSHANDNNIDDSTDSNIENEDYR
                     YNQEIDDQNESNGIAVGNEQPQSAPKEQNSDSNDEETVTKKERKSKVTQLKPLTLEEK
                     RKLRRKRQKRIQYSVITILVLLIAVILIYMFSPLSKIAHVNINGNNHVSTSKINKVLG
                     VKNDSRMYTFSKKNAINDLEEDPLIKSVEIHKQLPNTLNVDITENEIIALVKYKGKYL
                     PLLENGKLLKGSNDVKINDAPVMDGFKGTKEDDMIKALSEMTPEVRRYIAEVTYAPSK
                     NKQSRIELFTTDGLQVIGDISTISKKMKYYPQMSQSLSRDSSGKLKTRGYIDLSVGAS
                     FIPYRGNTSSQSESDKNVTKSSQEENQAKEELQSVLNKINKQSSKNN"
     gene            10779..12191
                     /gene="ftsA"
                     /locus_tag="PPY92_001172"
     CDS             10779..12191
                     /gene="ftsA"
                     /locus_tag="PPY92_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDJ2625185.1"
                     /translation="MEEHYYVSIDIGSSSVKTIVGEKFHNGINVIGTGQTYTSGIKNG
                     LIDDFDIARQAIKDTIKKASIASGVDIKEVFLKLPIIGTEVYDESNEIDFYEDTEING
                     SHIEKVLEGIREKNDVQETEVINVFPIRFIVDKENEVSDPKELIARHSLKVEAGVIAI
                     QKSILINMIKCVEACGVDVLDVYSDAYNYGSILTATEKELGACVIDIGEDVTQVAFYE
                     RGELVDADSIEMAGRDITDDIAQGLNTSYETAEKVKHQYGHAFYDSASDQDIFTVEQV
                     DSDETVQYTQKDLSDFIEARVEEIFFEVFDVLQDLGLTKVNGGFIVTGGSANLLGVKE
                     LLSDMVSEKVRIHTPSQMGIRKPEFSSAISTISSSIAFDELLDYVTINYHDNEETEED
                     VIDVKDKDNESKLGGFDWFKRKTNKKDTHENEVESTDEEIYQSEDNHQEHKQNHEHVQ
                     DKDKDKEESKFKKLMKSLFE"
     gene            12224..13396
                     /gene="ftsZ"
                     /locus_tag="PPY92_001173"
     CDS             12224..13396
                     /gene="ftsZ"
                     /locus_tag="PPY92_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDJ2625186.1"
                     /translation="MLEFEQGFNHLATLKVIGVGGGGNNAVNRMIDHGMNNVEFIAIN
                     TDGQALNLSKAESKIQIGEKLTRGLGAGANPEIGKKAAEESREQIEDAIQGADMVFVT
                     SGMGGGTGTGAAPVVAKIAKEMGALTVGVVTRPFSFEGRKRQTQAAAGVEAMKAAVDT
                     LIVIPNDRLLDIVDKSTPMMEAFKEADNVLRQGVQGISDLIAVSGEVNLDFADVKTIM
                     SNQGSALMGIGVSSGENRAVEAAKKAISSPLLETSIVGAQGVLMNITGGESLSLFEAQ
                     EAADIVQDAADEDVNMIFGTVINPELQDEIVVTVIATGFDDKPTSHGRKSGSTGFGTS
                     VNTSSNATSKDESFTSNSSNAQATDSVSERTHTTKEDDIPSFIRNREERRSRRTRR"
BASE COUNT         5001 a         1857 c         2614 g         4077 t
ORIGIN      
        1 tccggaattt tttaattatg tttttaatga tattggtgag gatgaaagac agcactcgat
       61 tatagttgga gattctttaa catctgacat tctaggtgga atcaatgcgg gtatagctac
      121 ttgctggttt aattttagag gatttgatca taatccagga attatacctg attatgaaat
      181 taattcatgg aaacaactaa atgatattgt acgttaagac aaatagtttg aaaatgattt
      241 acttatatta gcttaagaaa gtaattgtaa aaacgacact acgaaaagat gcattttcat
      301 gctcgtagtg tcgaatatta ttatcttatg gtttccacaa taagacatca tgtccctcta
      361 tatttttagc ttccacatct gtaaatccat gatgtaaaaa gaaatcctta gaatcatttc
      421 tgccaatggc tttaattggc atattgaaac tctttgcaaa atcaatcaat tctgaagcgt
      481 accctctgtt ttgatatttt ggtaatactt ctaacttcca taataatata taatcttcaa
      541 aatctgggaa gtagatttct tcgacatcac ctttttttag taatgccatt ctagctccta
      601 attgatctcc gacaaatatg ccataaaatg gtgaatctga acttgcatca atcatttgac
      661 cgtttaactc attgaccatg tataagtctt tgttgccaaa cgctctaaag ttttcgaata
      721 attcgtcagt tttgtaatta atttcaagac gtttgatttc actcatattt atctacccct
      781 tatttgttta ctactattat tatacattaa accacttgtg tttttcattg atttgaaaat
      841 gtaaaacaat gaacattata aattttccat gattaaattg attttcaagc cgtaaaattg
      901 tcaatcaccg gtatagactt tatactatag tttgtaatcg cgtacttaag gagaaaaaac
      961 atggactgta aagtagttag tttaaatgaa aaagatcagt ttataccaaa aataaagagc
     1021 agtgaccctg taataacagg attatttcaa tatgatgcag ctcaacaaac tagttttgaa
     1081 aaaaggatgt ctaaagaaaa taatggaaga gaagcggcat tagcgaatgt tattcgtgaa
     1141 tatatgagtg atttaaagct ttcaaatgaa caagaattaa acatacaaca tttagctaat
     1201 ggttcaaaag ttgtgattgg tggacaacaa gcagggcttt tcgggggacc attgtataca
     1261 ttccataaaa tattttcaat cattacttta tctaaggaat taacggatac acataagcaa
     1321 caagtagtac cagttttttg gattgcagga gaagatcatg atttcgatga agtgaatcat
     1381 acatttgttt ataacgaaaa tcatgggtcg ctgcataagg ttaaatatca tacaatggag
     1441 atgccagaga cgacagtctc tagatattat cctgataagg ctgagttgaa acaaacttta
     1501 aaaacgatgt tcattcatat gaaagaaact gttcatacac aaggtctact ggagatttgt
     1561 gacagaatta ttgaccaata tgactcgtgg actgatatgt ttaaagcact actgcatgaa
     1621 acatttaaag catatggcgt tctatttata gatgcgcagt ttgagccgtt aagaaaaatg
     1681 gaagcgccta tgtttaaaaa gattttgaaa aaacatcagt tgcttgatga tgcttttaga
     1741 gcaacacaac aacgtactca aaatcaaggc ttgaatgcga tgatacaaac agatacaaat
     1801 gttcatttat tcttacatga tgaaaatatg cgccaattag tttcgtatga tggtaagcat
     1861 tttaaattaa ataaaacaga taagacatat ataaaggaag aaattataaa tattgcggaa
     1921 aatcaacctg aattattttc taataatgta gtgacaagac cattaatgga agaatggtta
     1981 tttaacacgg tggcatttgt tggaggaccg agtgaaatta agtactgggc tgaactaaaa
     2041 gatgtatttg aactatttga tgttgaaatg cctatcgtga tgccaaggct tagaattact
     2101 tatttaaatg accgtataga aaaattactt tcgaaataca atattccatt agaaaaagtg
     2161 ttagtcgatg gtgttgaagg agaaagaagt aagtttatta gagaacaagc atcacatcaa
     2221 tttattgaaa aggtagaagg tatgattgaa caacagcgtc gtctaaacaa agacttatta
     2281 gatgaagtgg cggggaatca aaataatatt aaccttgtga ataaaaataa tgaaattcat
     2341 atacaacagt atgattattt gttaaaacgt tatcttttaa acattgaaag agaaaacgac
     2401 atcagtatga agcaatttag agaaattcaa gaaacactcc atccaatggg aggattacaa
     2461 gaaagaatat ggaatccact tcaaattttg aatgattttg ggacagatgt gttcaagccc
     2521 tccacctatc caccactttc ttacactttt gatcatatta ttataaaacc ttaatatacc
     2581 aagggtttag cccgatttat cttaatgata aatcgggcat ttttttgttt tttaaaataa
     2641 atttcacaaa ttttgtataa atagtggtgg atagtgggga gatgtggtaa attatatata
     2701 aggtgaggtg ataaaaaatg ttcatgggag aatacgatca tcaattagat acaaaaggac
     2761 gtatgattat accgtccaag tttcgttatg acttaaatga gcgttttatt atcacaagag
     2821 gccttgataa atgtttattc ggttacactc tagacgaatg gcaacagatt gaagagaaaa
     2881 tgaaaacctt acctatgaca aaaaaagacg cacgtaagtt tatgcgtatg ttcttctctg
     2941 gtgctgttga agtagaactt gataagcaag ggcgtattaa catccctcaa aacttgagga
     3001 aatacgctaa tttaactaaa gaatgtacag taatcggtgt ttcaaatcgt attgagattt
     3061 gggatagaga aacttggaat gatttctatg aagaatctga agaaagtttc gaagatattg
     3121 ctgaagattt aatagatttt gatttttaaa atggaggaat tgaagtgttt catcatatca
     3181 gcgttatgtt aaacgaaacc attgattatt taaatgtaaa agaaaatggt gtgtacattg
     3241 actgtacgct aggtggagcg ggacatgccc tttatttact aaatcaatta aatgacgacg
     3301 gaagattaat agcaatcgat caagaccaaa ctgcaattga taatgctaaa gaggtattaa
     3361 aggatcattt gcataaggtg acttttgttc atagcaactt ccgtgaatta actcaaatat
     3421 taaaagactt aaacattgaa aaagtagatg gaatttatta cgacttgggt gtttcaagcc
     3481 cacaactcga cattccagaa cgaggattca gttatcacca tgacgcaaca ttagacatgc
     3541 gtatggacca aacacaagaa ctaacagcat atgaaattgt taacaattgg tcatatgaag
     3601 cgttagtgaa gattttttat cgttatggcg aggagaaatt ttcaaaacag atagctcgaa
     3661 gaatcgaagc acatcgtgaa caacaaccaa taacaacaac attagaatta gttgacatta
     3721 taaaagaagg tattcctgca aaagcaagaa gaaaaggcgg acatcctgca aaacgagtat
     3781 ttcaagcact acgaattgca gtaaacgatg aattgtcagc ttttgaagat tcaatagaac
     3841 aagcgattga attagtgaaa gtagacggca ggatttcggt aatcactttc cattctttag
     3901 aagatcgttt atgtaaacag gtgttccaag aatatgaaaa aggtccagag gtaccaagag
     3961 gattgccagt tataccagaa gcatatacac ctaagttaaa gcgtgttaat cgtaaaccga
     4021 ttaccgctac agaagaagat ttagatgaca ataacagagc acgaagcgcg aaattacgtg
     4081 tagctgaaat acttaaataa ggagcaattt ataatggctg tagaaaaagt gtaccaacca
     4141 tatgacgaac aagtttataa tagtataccg aagcaacaac cacaaactaa gcccgaaaag
     4201 aagactgttt cgagaaaagt ggttgtacaa ttaactaaat ttgaaaaagt tttatacata
     4261 actttgatta ctgtaattgc tatgttaagt atttatatgc tatctttaaa aatggatgcg
     4321 tatgatacgc gaggaaagat tgcagattta gattataaaa tagataaaca atcaagtgaa
     4381 aacagtgctt tacaatctga aatcaaaaag aattcttctt atgaacgcat atacgaaaag
     4441 gctaagaaac aggggatgag ccttgagaac gataatgtaa aggtagtgcg tagtaatggc
     4501 gaagcaaaaa attaaaatta aaaaaaataa aataggggca gtcctacttg ttggtttatt
     4561 cggactgctc ttttttatat tggttttaag aatttcatat atcatgatta ctggacattc
     4621 taatggtcaa gatttagtca tgaaggcaaa tgaaaagtat ttagttaaga atgcacaaca
     4681 accagaacga ggaaagatat atgatcgtaa tggtaaagtg ctagcagaag atgtagaaag
     4741 atataaactt gttgcagtaa tagataaaaa ggcgagtgcc aattctaaaa aacctaggca
     4801 tgtagttgat aaaaaagaga ctgcaaagaa attatctaca gtcattgata tgaagccaga
     4861 ggaaattgaa aagagactta gtcaaaagaa agctttccaa attgaatttg gacgcaaagg
     4921 aacaaattta acgtatcagg acaaattgaa aatagagaaa atgaatttgc ctggtatttc
     4981 tttattgcct gaaacagaac gcttttatcc aaatggcaat tttgcatcac acttaattgg
     5041 tagagctcag aaaaatccgg atactggtga acttaaaggt gcacttggag ttgaaaagat
     5101 ttttgatagt tatttaagtg gatctaaagg atcattgaga tatattcatg atatttgggg
     5161 atatatcgca ccaaatacta aaaaagagaa gcagcctaaa cgtggtgatg atgtccattt
     5221 aacaatcgat tcaaatattc aagtatttgt tgaagaagct ttagatggca tggttgaaag
     5281 ataccagccg aaagatttat ttgcggttgt catggatgcc aaaactggag aaattttagc
     5341 atacagtcag cgaccaacat ttaatcctga aactggtaaa gactttggta aaaagtgggc
     5401 aaatgacctt tatcaaaaca catacgagcc tggatcaaca tttaaatcat atgggttagc
     5461 agctgctatt caagaaggtg cttttgatcc tgataagaaa tataaatctg gacatagaga
     5521 tattatgggt tcacgtattt cagactggaa tagagtcggt tggggtgaaa tcccaatgtc
     5581 actcggattt acttattcat ctaatacatt gatgatgcat ttacaagatt tagttggtgc
     5641 agacaaaatg aaatcttggt atgaacgatt tggatttgga aaatcaacta aaggtatgtt
     5701 tgatggagaa gcacctggtc aaattggatg gagtaatgag ttgcaacaaa aaacgtcatc
     5761 atttggccaa tcgacaacag taacacctgt tcaaatgtta caggcgcaat cagcgttctt
     5821 taatgatggt aatatgttaa aaccatggtt tgtgaatagc gttgaaaatc ctgttagtaa
     5881 aagacaattt tataaaggtc aaaaacaaat cgcaggcaaa ccaataacaa aagatactgc
     5941 tgaaaaagtt gaaaagcaat tggatttagt tgtgaatagt aagaagagtc acgctgcaaa
     6001 ctatcgtatt gatggttatg aggtcgaagg taagactggt acagcacaag tcgctgcacc
     6061 taatggtggt ggatacgtta aaggtccaaa cccatatttt gtaagtttta tgggtgacgc
     6121 accgaagaaa aatcctaaag ttattgtata cgctggtatg agcttggcac aaaaaaatga
     6181 ccaagaagct tatgaattag gtgttagtaa agcgtttaaa ccaataatgg aaaatacttt
     6241 gaaatattta aatgtaggta aatcaaaaga tgacacatct aatgcagagt atagtaaagt
     6301 gccagatgtt gaaggtcaag acaaacaaaa agctattgat aatgtgagtg caaaatcatt
     6361 agaaccagtt actatcggtt ctggcacaca aataaaagca caatctataa aagcagggaa
     6421 taaagtctta cctcatagta aagtactgtt attaacagac ggagacttaa ctatgcctga
     6481 catgtcagga tggacgaaag aagatgtcat tgcttttgaa aacctaacaa atattaaagt
     6541 aaatttaaaa ggtagcggtt ttgtgtccca ccaatcaatt agtaagggac aaaaacttac
     6601 tgaaaaagat aaaatagacg tagaattttc atcagagaat gtagacagca attcgacgaa
     6661 taattctgat tcaaattcag atgataagaa gaaatctgac agtaaaactg acaaggataa
     6721 gtcggactaa caagaatggc tacattaaat aagggtgtat tgtcactatt tgtttttact
     6781 aaagtaatgc tgtgatagaa aaaaacatct tcatttaatc tttataaata agtgttgata
     6841 gagaatttta aggtagacga ggtcagatta acttgtttgt tataggcaaa ctttttatac
     6901 tggcctctta tgccgtaaaa aagaaaaatt ataaattttg attacaactt tgtttattta
     6961 attgaaaaat tgataatata ttagtggtta aaaaagtctg gattaagaag aggagatagt
     7021 tatgattttt gtatatgcgt tattagcgct agtgattaca tttgttttgg tacctgtttt
     7081 aatacctaca ttaaaaagga tgaaatttgg tcaaagtatt cgagaagaag gtccacaaag
     7141 ccatatgaag aagactggta caccaacgat gggtggacta acatttctat taagtattgt
     7201 gataacgtct ttggtggcta ttatatttgt agatcaagct aatccaatca tactgttatt
     7261 atttgtgacg attggttttg ggttaattgg ttttatagat gattatatta ttgttgttaa
     7321 aaagaataac caaggtttaa caagtaaaca gaagtttttg gcgcaaattg gtattgcgat
     7381 tattttcttt gttttaagta atgtgtttca tttggtgaat ttttctacga gcatacatat
     7441 tccatttacg aatgtagcaa tcccactatc atttgcatat gttattttca ttgttttttg
     7501 gcaagtaggt ttttctaatg cggtaaattt aacagatggt ttagatggat tagcaactgg
     7561 actgtcaatt atcggattta caatgtatgc catcatgagc tttgtgttag gagaaacggc
     7621 aattggtatt ttctgtatca ttatgttgtt tgcactttta ggatttttac catataacat
     7681 taaccctgct aaagtgttta tgggagatac aggtagctta gctttaggtg gtatatttgc
     7741 tacgatttca atcatgctta atcaggaatt atcattaatt tttataggtt tagtattcgt
     7801 aattgaaaca ttatctgtta tgttacaagt cgctagcttt aaattgactg gaaagcgtat
     7861 atttaaaatg agtccgattc atcatcattt tgaattgata ggatggagcg aatggaaagt
     7921 agttacagta ttttgggctg ttggtctgat ttcaggttta atcggtttat ggattggagt
     7981 gcattaagat gcttaattat acagggttag aaaataaaaa tgtattagtc gtcggtttgg
     8041 caaaaagtgg ttatgaagca gctaaattat taagtaaatt aggtgcgaat gtgactgtca
     8101 atgatgggaa agacttatcg caagatgctc atgcaaaaga tttagaatct atgggtattt
     8161 ctgttgtaag tggaagtcat ccattaacat tgcttgataa taatccaata attgttaaaa
     8221 atcctggaat accttataca gtatctatta ttgatgaagc agtgaaacga ggtttgaaaa
     8281 ttttaacaga agttgagtta agttatctaa tctctgaagc accaatcata gctgtaacgg
     8341 gtacaaatgg taaaacgaca gttacttctc taattggaga tatgtttaaa aaaagtcgct
     8401 taactggaag attatccggc aatattggtt atgttgcatc taaagtagca caagaagtaa
     8461 agcctacaga ttatttagtt acagagttgt cgtcattcca gttacttgga attgaaaagt
     8521 ataaaccaca cattgctata attactaaca tttattcggc gcatctagat taccatgaaa
     8581 atttagaaaa ctatcaaaat gctaaaaagc aaatatataa aaatcaaacg gaagaagatt
     8641 atttgatttg taattatcat caaagacaag tgatagagtc ggaagaatta aaagctaaga
     8701 cattgtattt ctcaactcaa caagaagttg atggtattta tattaaagat ggttttatcg
     8761 tttataaagg tgttcgtatt attaacactg aagatctagt attgcctggt gaacataatt
     8821 tagaaaatat attagcagct gtgcttgctt gtattttagc tggtgtacct attaaagcaa
     8881 ttattgatag tttaactaca ttttcaggaa tagagcatag attgcaatat gttggtacta
     8941 atagaactaa taaatattat aatgattcca aagcaacaaa cacgctagca acacagtttg
     9001 ccttaaattc atttaatcaa ccaatcattt ggttatgtgg tggtttggat cgagggaatg
     9061 aatttgacga actcattcct tatatggaaa atgttcgcgc gatggttgta ttcggacaaa
     9121 cgaaagctaa gtttgctaaa ctaggtaata gtcaagggaa atcggtcatt gaagcgaaca
     9181 atgtcgaaga cgctgttgat aaagtacaag atattataga accaaatgat gttgtattat
     9241 tgtcacctgc ttgtgcgagt tgggatcaat atagtacttt tgaagagcgt ggagagaaat
     9301 ttattgaaag attccgtgcc catttaccat cttattaaag ggtgtgagta ttgatggatg
     9361 ataaaacgaa gaacgatcaa caagaatcaa atgaagataa agatgaatta gaattattta
     9421 cgaggaatac atctaagaaa agacggcaaa gaaaaaggtc aaaggctaca catttttcta
     9481 atcaaaataa agatgataca tctcaacaag ctgattttga tgaagaaatt tacttgataa
     9541 ataaagactt caaaaaagaa gaaagcaatg atgaaaataa tggttctgct tctagtcatg
     9601 cgaatgataa taatatcgat gattctacag actctaatat tgaaaatgag gattatagat
     9661 ataatcaaga aattgacgac caaaatgaat cgaatggaat tgcagtcggc aacgaacaac
     9721 ctcaatcagc tcctaaagaa caaaatagcg actcgaatga tgaggaaaca gtaacgaaaa
     9781 aagaacgaaa aagtaaagta acacaattaa agccattaac acttgaagaa aagcggaagt
     9841 taagacgtaa gcgacaaaaa cgaatccaat acagtgttat tacaatatta gtattgttga
     9901 ttgctgttat attaatttac atgttttcac cacttagtaa aattgcgcat gtaaatataa
     9961 atggaaataa tcacgttagt acttcaaaga taaacaaagt tttaggtgtt aaaaatgatt
    10021 cgaggatgta tacgtttagt aaaaaaaatg ctattaatga tctcgaagag gatccattaa
    10081 tcaaaagtgt tgagatacac aagcaattac caaacacatt aaacgtagat atcacagaaa
    10141 atgaaattat tgctttagtg aaatataaag gtaaatattt acctttatta gaaaatggta
    10201 aattgcttaa aggttcaaat gatgtcaaaa ttaatgatgc acctgtcatg gatggtttca
    10261 aaggtacaaa agaagatgat atgattaagg cgttatctga aatgacacct gaagttagac
    10321 gatatattgc cgaagtgaca tacgccccaa gtaaaaacaa acaaagcaga attgaattgt
    10381 ttacgacaga tggacttcaa gtaatcggtg atatttcgac gatatctaag aaaatgaaat
    10441 attatccgca gatgtcacaa tcattatcaa gggatagttc gggtaaacta aaaacaagag
    10501 gctatattga tttatcagtc ggtgcttcat ttatcccata ccgtggaaac acgtctagtc
    10561 aatcagaaag cgataaaaat gtgactaaat catctcaaga ggaaaatcaa gcaaaagaag
    10621 aattacaaag cgttttaaac aaaattaaca aacaatcaag taagaataat taaaaaattt
    10681 tgatattgtc tatgtttata gttcacaagc cattcaacgt attgtaaact aaggatagtg
    10741 tattttttta atagtaattt gtcaggaggt gcctatctat ggaagaacat tactacgtaa
    10801 gtattgatat tggatcatca agcgtaaaaa caatagtagg cgagaaattt cacaatggta
    10861 taaatgtgat aggtacagga caaacctaca cgagcggtat aaaaaatggt ttaattgatg
    10921 attttgatat tgcgcgacaa gcaatcaaag acacaattaa aaaggcatca atcgcttcgg
    10981 gtgttgatat taaagaagtt ttcctgaaat tacctatcat tggaacggaa gtttatgatg
    11041 aatcaaatga aatcgacttt tatgaggata cagaaatcaa cggttcacat atcgaaaaag
    11101 tattagaagg tattagagaa aaaaatgatg tgcaagaaac agaagtaatt aatgtgttcc
    11161 cgattcgttt tatagtcgat aaagaaaatg aggtttcaga ccctaaagaa ttaattgcca
    11221 gacattcatt aaaggttgaa gcaggcgtaa ttgctattca aaaatcgatt ttaattaata
    11281 tgattaaatg cgtagaagca tgtggtgttg atgtattaga tgtttactct gatgcatata
    11341 actatggttc aatcctaaca gctactgaaa aagagttagg tgcatgtgtc attgatattg
    11401 gtgaagacgt tacgcaagtt gctttttatg aacgcggtga attagtagat gctgattcta
    11461 tcgaaatggc agggcgtgat attacagacg atattgcaca aggattaaac acttcttatg
    11521 aaactgctga aaaagttaaa caccaatatg gtcatgcatt ctatgattct gcttcagatc
    11581 aagatatctt cactgttgaa caggttgata gtgatgaaac agtacagtat actcaaaaag
    11641 atttgagtga ctttattgaa gcgcgtgtag aagaaatatt cttcgaagta tttgatgttt
    11701 tacaagattt aggattaaca aaagtaaatg gtgggtttat tgtaactggt ggatctgcaa
    11761 acttacttgg cgtaaaagaa ttattatcag atatggtaag tgaaaaagtt agaattcaca
    11821 cgccatcaca aatgggaatt agaaaacctg aattttcttc agcaatttct acaatttcta
    11881 gtagtatcgc ttttgatgag ttattagatt atgttacaat taattatcat gataatgaag
    11941 aaactgaaga agatgttatt gatgtgaaag acaaagataa cgaatctaaa ttaggcggat
    12001 ttgattggtt taaacgtaaa acaaacaaaa aagatactca tgaaaatgaa gtagagtcaa
    12061 cagatgaaga aatttatcaa tcagaagata atcatcagga acataaacag aatcatgaac
    12121 atgttcaaga caaagataaa gataaagaag aaagtaaatt caaaaaacta atgaaatctc
    12181 tatttgaatg attattggcc aataaaacta ggaggaaatt taaatgttag aatttgaaca
    12241 aggatttaat catttagcga ctttaaaggt cattggtgta ggtggtggcg gtaacaacgc
    12301 cgtaaaccga atgattgacc acggaatgaa taatgttgaa tttatcgcta tcaacacaga
    12361 cggtcaagct ttaaacttat ctaaagctga atctaaaatc caaatcggtg aaaaattaac
    12421 acgtggttta ggagcaggag ctaaccctga aatcggtaaa aaagctgcag aggaatctcg
    12481 tgaacaaatt gaagatgcaa tccaaggtgc agacatggta tttgttactt ctggtatggg
    12541 tggcggaact ggtactggtg cagcaccagt cgttgctaaa attgcaaaag aaatgggcgc
    12601 attaactgtt ggtgttgtaa ctcgtccatt tagttttgaa ggacgtaaac gtcaaactca
    12661 agctgctgct ggagtagaag ctatgaaagc tgcagtagat acattaatcg ttataccaaa
    12721 tgaccgttta ttagatatcg ttgacaaatc tacgccaatg atggaagcat ttaaagaagc
    12781 tgataacgtg ttacgccaag gtgtacaagg tatctcagac ttaatcgctg tttctggtga
    12841 agtaaactta gactttgcag acgttaagac aattatgtct aaccaaggtt ctgcattaat
    12901 gggtattggt gtttcttctg gtgaaaatag agcggtagaa gctgctaaaa aagcaatctc
    12961 ttctccatta cttgaaacat ctatcgttgg tgcacaaggt gtgcttatga atattactgg
    13021 tggcgagtca ttgtcattat ttgaagcaca agaggctgct gatattgtcc aagatgctgc
    13081 agatgaagac gttaatatga ttttcggtac agttattaat cctgaattac aagatgagat
    13141 tgttgtaaca gttattgcaa ctggttttga tgacaaacca acatcacatg gtcgtaaatc
    13201 tggtagcact ggattcggaa caagcgtaaa tacttctagc aatgcaactt ctaaagatga
    13261 atcattcact tcaaattcat caaatgcaca agcaactgat agtgtaagtg aaagaacgca
    13321 tacaactaaa gaagatgata ttccaagctt cattagaaat agagaagaaa gacgttcaag
    13381 aagaacaaga cgttaatcgg ttaatatata tacacaaata attcaacaca aatcatcaga
    13441 taacatatct gatgattttt ttactaattt ttagaacatg tagaaggaca tttaagtttt
    13501 tcgaagtcat taaaagtgtt taagtatcgt gtcaaaatta agtcaaaaa
//
LOCUS       DANBWH010000059        13515 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.061, whole genome shotgun sequence.
ACCESSION   DANBWH010000059 DANBWH010000000
VERSION     DANBWH010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13515
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.061"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            36..170
                     /gene="graF"
                     /locus_tag="PPY92_001174"
     CDS             36..170
                     /gene="graF"
                     /locus_tag="PPY92_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance-associated protein GraF"
                     /protein_id="HDJ2625187.1"
                     /translation="MSNENQNKKAAEKAKEVEEKLKDKKEEKTEDINQTKQDIQDTLN
                     "
     regulatory      829..929
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1006..1581
                     /locus_tag="PPY92_001175"
     CDS             1006..1581
                     /locus_tag="PPY92_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDJ2625188.1"
                     /translation="MSKGLKLSEILVTVLISVVFAVIYNLWWFVYNGVQATGLHLEQL
                     TNGVWFMAAIVCYLIIPKPGIALLAEFAAGAGETIIMGRFDIPTIVYAFIQGLACELV
                     FAIFKYQSRSVMVAMLAGFCTAIAAFPIDYFYGYLNEVAGWNLTLFIVFRLISGAVVA
                     GVLSYLLVKALDKTGVTKLFRPAAKEDYDNL"
     gene            1596..2996
                     /locus_tag="PPY92_001176"
     CDS             1596..2996
                     /locus_tag="PPY92_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDJ2625189.1"
                     /translation="MLKVSDLRLKYPNGQRKIFDHLNITIQDKEKVLLLGPSGCGKST
                     LLNVLSGIVPNLIELPMKYDELIVDPLSGVIFQDPDSQFCMPKVYEELAFVLENRQVP
                     REDMDALIINALNMVNLNVTPETYIKDLSGGMKQKLAIVETILQQSKTLFLDEPTAML
                     DVQATEDLWTKLIELWEDQTVVIVEHKVKHIWNHVDRVILMDYNGNIIADECPEIILQ
                     KYVHLLSEYGVWHPRAWEFAPSRVDFPTTNSHLLQFKNGRIIRGKSTLLSFSDLEIGL
                     GEWITITGANGSGKTTLLESIMQLIKYQGDVYFENQRLTKIKHAAKHMYLVYQNPELQ
                     FITNSVYDEINIHFNHLSKDQSDDETIQLLKLLDLQNVKDQHPYELSIGQKRRLSVAT
                     ALSSKADIIFLDEPTFGLDSHNTFQLIKLFQKRINLGQSIVMVTHDDEIIERYPSRRL
                     KISDGALLDCDGDTNV"
     gene            2989..3795
                     /locus_tag="PPY92_001177"
     CDS             2989..3795
                     /locus_tag="PPY92_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDJ2625190.1"
                     /translation="MYEQWKVRQSFVDDVNIITKLFLGIILFFFIIFVHNFDFMLYIT
                     ILMLVFLLMFNGTQFKITAIFITISIGFALLSSLFMILYGNGSHTILKFGFIHITTES
                     LVRGLHVSLRTIAISFFGILIALTSQVVMIFYSLMQHLKVKSKVAYAFMAAIRMIPLM
                     ISSLIQLRRSLKMRYQMIDAANYRGLKRFKHLIIPLLSQNIRKAHQLSVAMESKGFRD
                     GPRTYYYNVPFSYKDIIFIIVLIAIITMAYYCSITLPITGIDDVRLGRVG"
     gene            complement(4062..5309)
                     /locus_tag="PPY92_001178"
     CDS             complement(4062..5309)
                     /locus_tag="PPY92_001178"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDJ2625191.1"
                     /translation="MNVLVIGAGGREHALAYKLNQSNLVKQEFVIPGNEAMTPIAEVH
                     TEISESNHQGILDFAKQQNVDWVVIGPEQPLIDGLADILRANGFKVFGPNKQAAQIEG
                     SKLFAKKIMKKYNIPTADYKEVERKKDALTYIENCELPVVVKKDGLAAGKGVIIADTI
                     EAARSAIEIMYGDEEEGTVVFETFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHD
                     EGPNTGGMGAYCPVPHISDDVLKLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKDG
                     PKVIEFNARFGDPEAQVLLSRMESDLMQHIIDLDEGKRTEFKWKNESIVGVMLASKGY
                     PDAYEKGHKVSGFDLNENYFVSGLKKQGDTFVTSGGRVILAIGKGDNVQDAQRDAYEK
                     VSQIQSDHLFYRHDIANKALQLK"
     gene            complement(5331..6809)
                     /gene="purH"
                     /locus_tag="PPY92_001179"
     CDS             complement(5331..6809)
                     /gene="purH"
                     /locus_tag="PPY92_001179"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDJ2625192.1"
                     /translation="MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVP
                     VRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLY
                     PFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYHEVLTRLRNDS
                     LDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIA
                     GAKQLHGKQLSYNNIKDADATLALVKKFDTPAAVAVKHMNPCGVGIGDTIEQAFQHAY
                     EADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEALDILKQKKNVRLLEI
                     DMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPS
                     VKSNAIILSNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVEL
                     AAQHGIKAIIQPGGSIKDQDSIDMANKHGIAMVVTGTRHFKH"
     gene            complement(6824..7390)
                     /gene="purN"
                     /locus_tag="PPY92_001180"
     CDS             complement(6824..7390)
                     /gene="purN"
                     /locus_tag="PPY92_001180"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDJ2625193.1"
                     /translation="MVKIAIFASGSGSNFENIVEHVESGKLENIEVTALYTDHQNAFC
                     IDRAKKHDIPVYINEPKQFDSKAAYEQHLVTLLNKDKVEWIILAGYMRLIGPDLLASF
                     EGKILNIHPSLLPKYKGIDAIGQAYHSGDTITGSTVHYVDCGMDTGEIIEQRQCDIRP
                     DDSKEQLEEKVKKLEYELYPSVIAKIVK"
     gene            complement(7393..8421)
                     /locus_tag="PPY92_001181"
     CDS             complement(7393..8421)
                     /locus_tag="PPY92_001181"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDJ2625194.1"
                     /translation="MSKAYEQSGVNIHAGYEAVERMSSHVKRTMRKEVIGGLGGFGAT
                     FDLSQLNMTAPVLVSGTDGVGTKLKLAIDYGKHDSIGIDAVAMCVNDILTTGAEPLYF
                     LDYIATNKVVPEVIEQIVKGISDACVETNTALIGGETAEMGEMYHEGEYDVAGFAVGA
                     VEKDDYVDGSEVKEGQVVIGLASSGIHSNGYSLVRKLINESGIDLASNFDNRPFIDVF
                     LEPTKLYVKPVLALKKEVSIKAMNHITGGGFYENIPRALPAGYAARIDTTSFPTPKIF
                     DWLQQQGNIDTNEMYNIFNMGIGYTVIVDEKDASRALKILAEQNVEAYQIGHIVKNES
                     TAIELLGV"
     gene            complement(8414..9898)
                     /locus_tag="PPY92_001182"
     CDS             complement(8414..9898)
                     /locus_tag="PPY92_001182"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDJ2625195.1"
                     /translation="MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIV
                     VSDQNELKGERGLGLLTEAINDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHF
                     YDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKES
                     LRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQ
                     DIHAGEYVVINDKGITVKSYTHHTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKK
                     LAQESPVNADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYVARTFIQPTQELREQ
                     GVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFP
                     SFYGIDVSTTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVE
                     SFTGDYPAGLYDYEANYKAHLSHRQKQYISKNKHFFDSEGNLNV"
     gene            complement(9877..12066)
                     /gene="purL"
                     /locus_tag="PPY92_001183"
     CDS             complement(9877..12066)
                     /gene="purL"
                     /locus_tag="PPY92_001183"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HDJ2625196.1"
                     /translation="MSKFIEPSVEEIKLEKVYQDMGLSDQEYEKVCDILGRQPNFTET
                     GIFSVMWSEHCSYKHSKPFLKQFPTSGEHVLMGPGEGAGVVDIGDNQAVVFKVESHNH
                     PSAIEPYQGAATGVGGIIRDIVSIGARPINLLNSLRFGELDNKQNQRLLKGVVKGIGG
                     YGNCIGIPTTAGEIEFDERYDGNPLVNAMCVGVINHDMIQKGTAKGVGNSVIYVGLKT
                     GRDGIHGATFASEELTEESESKRPSVQIGDPFVGKKLMEATLEAITFDELVGIQDMGA
                     AGLTSSSSEMAAKGGSGLHLRLEQVPTREPGISPYEMMLSETQERMLLVVEKGNEQKF
                     LDLFDKHELDSAVIGEVTDTNRFVLTYDDEVYADIPVEPLADEAPVYILEGEEKDYNT
                     SKNDYTHIDVKDTFFKLLKHPTIASKHYLYDQYDQQVGANTIIKPGLQASVVRVEGTN
                     KAIASTIDGEARYVYNNPYEGGKMVVAEAYRNLIAVGATPLAMTDCLNYGSPEKKEIY
                     QQLIDSTKGMAEACDILKTPVVSGNVSLYNETKGTSIFPTPVVGMVGLIENVNYLNDF
                     EPQVGDKLYLIGDTKDDFGGSQLEKLIYGKVNHEFESLDLSSEVEKGESIKTAIREGL
                     LSHVQTVGKGGLLITLAKLSAHYGLGLKSSIDITNAQLFSETQGRYVVSVKSGKTLNI
                     DNAIEIGLLTDSDNFKVTTPYTEISENVSDIKQIWEGAIAQCLTTQD"
     gene            complement(12059..12730)
                     /gene="purQ"
                     /locus_tag="PPY92_001184"
     CDS             complement(12059..12730)
                     /gene="purQ"
                     /locus_tag="PPY92_001184"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HDJ2625197.1"
                     /translation="MKFAVLVFPGSNCDRDMFNAAIKSGVEAEYVDYRETSLSGFDGV
                     LIPGGFSFGDYLRSGAMASVAPIISEVKRLATEGKPVLGVCNGFQILTEIGLLPGALL
                     HNDSHLFISRNEELEIVNNQTAFTNLYEQGEKVIYPVAHGEGHYYCTDEIYQQLKANN
                     QIILKYVNNPNGSYDDIAGIVNEKGNVCGMMPHPERALETLLGTDSGVKLFEAMVKSW
                     REQHV"
     gene            complement(12732..12995)
                     /gene="purS"
                     /locus_tag="PPY92_001185"
     CDS             complement(12732..12995)
                     /gene="purS"
                     /locus_tag="PPY92_001185"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HDJ2625198.1"
                     /translation="MKTIELHITLQPQVLDTQGQTLTRAVHDLGYAQVNDIRVGKVLY
                     MTVDEVSDEKVHNIITTLSEKLFANTVIEEYSYKVLDDEKENA"
     gene            complement(12995..>13515)
                     /locus_tag="PPY92_001186"
     CDS             complement(12995..>13515)
                     /locus_tag="PPY92_001186"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDJ2625199.1"
                     /translation="IESHFIKQLSETEQLVKPVKIIPLEVVVRNIASGSITKRLGFEN
                     GEVFREPLVEFFYKNDALNDPLITDDHVKLLNIASDEDIEILKSKALKINNVLKQLMD
                     AMNLKLVDFKIEFGKTETGQILLADEISPDTCRIWDKATNANFDKDVYRNNTGSLIET
                     YQIFLNKLEDLK"
BASE COUNT         4168 a         2650 c         1972 g         4725 t
ORIGIN      
        1 atataaataa ttatccaatt tgggaggaat taatcatgtc taatgaaaat caaaataaaa
       61 aagcagcaga aaaagctaaa gaagttgaag aaaaattaaa agataaaaaa gaagaaaaaa
      121 cagaagatat taatcaaaca aaacaagata tccaagatac actaaattaa tttaaatccc
      181 cttacaactt ttagttgtta ggggctttta tgcatgtgct cggcgcatgt gttcctcttc
      241 ttccttacaa cttttagttg ttaggggatt ttatgcatgt gctcggcgca tgtatccctc
      301 ttttacctta caacttttag ttgttagggg attttatgca tgtgctcggt gcatgtatcc
      361 ctcttcttcc ttacaacctt tagttgttag gggctttttt gcttgcgctc ggtgcatgta
      421 tccctctttt accttacaac ttttagttgt taggggcttt tttgcatgtg ctcggcgcat
      481 gtatccctct tttaccttac aacttttagt tgttaggggc ttttttgcat gtgctcggcg
      541 catgtatccc tcttttaccc tacaaactat aattcgtaag ccattatgca cgggttcttc
      601 actgttgtta aagaggtatg attgataagt atccctcttc atttatcagc tcaatcttat
      661 tacatgtcac atatttttat atctttaatt actaacattc ctactacatc ttcaatttaa
      721 atccaatgct aatattataa attttaaatc gaattaaacg aaatttcatg aagacaacct
      781 attgacataa gtttacaaac gtcttacagt ataattaatt aaataaatcg cacacactag
      841 gggtgtttta tactgagatg aggcttgccc tcaaaccctt tgaacctgat ctagcttgaa
      901 ctagcgtagg aaagtgttac tatacatatg ttttactaat atatattgtg aacgcataac
      961 tttcctatgg atggttgtgc gtttttttat taggaggatg taaaaatgtc aaaaggttta
     1021 aagctatctg aaatactagt aaccgtactt atttcagtag tattcgcagt tatttacaat
     1081 ttatggtggt ttgtttacaa tggtgtgcaa gctacaggcc ttcacctcga acaattaaca
     1141 aacggggttt ggttcatggc agcgattgtc tgctatctaa ttattccaaa accaggtatt
     1201 gctttactgg ctgaatttgc tgcaggtgcc ggcgaaacta tcattatggg tcgtttcgat
     1261 attccgacaa tcgtttacgc tttcatacaa ggtttagcgt gtgaacttgt ctttgcaatt
     1321 tttaaatatc aatctcgttc agttatggta gctatgttag caggtttttg tacagcaatc
     1381 gcggctttcc ctatagatta tttctatggt tatttaaatg aagttgcagg ttggaactta
     1441 acattattca tcgtatttag attaattagt ggtgcagtag tcgccggcgt cctatcttac
     1501 cttttagtaa aagctttaga taagacaggc gtaacaaaat tatttagacc agctgctaaa
     1561 gaagattacg acaacttata agaggagcat tcagagtgtt aaaagtaagt gatttacgat
     1621 taaaatatcc aaatggtcag cgtaaaattt tcgatcattt aaatatcact attcaagaca
     1681 aagaaaaagt acttttactc ggtccttctg gttgcggtaa aagtacactt ctgaatgtat
     1741 taagtggtat tgttcctaat ttaattgaat tacctatgaa atatgatgaa ctaatcgttg
     1801 acccattaag tggcgttatt ttccaagacc ctgatagcca gttttgtatg ccaaaagtat
     1861 acgaagaact tgcattcgtt ttagaaaata gacaagtacc acgtgaagac atggatgcgt
     1921 taattatcaa tgctttaaat atggtcaatt taaatgttac ccctgaaacg tatatcaaag
     1981 atttaagtgg cgggatgaaa cagaaattgg caattgttga aaccattctt caacaatcaa
     2041 aaacattgtt tttagatgaa ccgacagcaa tgttagatgt tcaagcaaca gaagatttat
     2101 ggactaaact aattgaactt tgggaagatc aaactgttgt aatcgttgaa cataaagtta
     2161 aacacatctg gaatcatgtc gaccgcgtca ttttgatgga ttataacgga aatatcattg
     2221 ccgatgaatg tcctgaaatc atattacaga agtatgttca tttactcagt gaatatggtg
     2281 tgtggcatcc acgtgcatgg gaattcgcac caagtcgtgt tgactttcca acaacaaact
     2341 cacacttatt acaatttaaa aatggacgta ttattcgcgg taaatcaaca ttgctctcat
     2401 tctcagattt agaaattggt ctaggtgagt ggattacaat tacaggggca aatggtagtg
     2461 gtaaaacaac cttgcttgaa tcaattatgc aattgattaa atatcaaggt gatgtttatt
     2521 ttgaaaatca gcgtttaaca aaaattaaac atgcagcaaa acacatgtac ctagtttatc
     2581 aaaacccaga attacaattt ataacaaatt cggtttatga tgaaattaac attcatttta
     2641 atcacctttc taaagatcaa agtgatgatg aaacgataca acttttaaaa cttttagatt
     2701 tacaaaatgt aaaagatcaa catccttatg agttgtctat tggtcaaaaa cgacgcctta
     2761 gcgtagctac cgcactaagt tctaaagctg atattatctt tttagatgaa ccgacatttg
     2821 gacttgatag ccataataca ttccagttga tcaaactttt ccaaaagcga attaatttgg
     2881 gacaaagtat tgtcatggtt acacatgacg atgaaataat tgaacgttat ccatcaagaa
     2941 gacttaaaat ttcagatggt gcgttactag attgtgatgg tgatacgaat gtatgagcaa
     3001 tggaaagttc ggcaatcatt tgttgatgat gtcaacatta taacgaaact atttcttgga
     3061 attatactat ttttctttat tatatttgtg cataatttcg attttatgct ttatatcact
     3121 atcttaatgt tagttttctt attgatgttt aatggtactc agtttaaaat tacagcaatt
     3181 tttataacta taagtattgg ctttgcatta ctctcttccc tgttcatgat tttatacggg
     3241 aatggtagcc atacaatttt gaaattcgga tttatccata ttacaacaga aagtttagtt
     3301 cgtggtttac atgtatcatt aagaaccatt gctatttcat ttttcggtat cttaatcgca
     3361 cttacatctc aggttgtcat gatattttat agtttaatgc aacatttaaa ggttaaatca
     3421 aaagttgctt atgcattcat ggctgccatt cgtatgatac ctttaatgat tagttcttta
     3481 atacagcttc gcagatcttt aaaaatgcgc tatcagatga tagacgcagc aaattataga
     3541 gggttaaaga gatttaagca tttaattata cctctattaa gccaaaatat tagaaaggca
     3601 catcaattat cagttgcgat ggagtcgaaa ggatttagag atggtcctcg tacttactat
     3661 tacaacgtac ctttctcata taaagatata atctttatta tagttttaat tgccattatt
     3721 actatggcat attattgttc aatcacctta cccatcacgg gcatagatga tgttcgtctt
     3781 ggtcgtgtag gttaaatttt atccataatt gtatagatac taaattagaa attcattgta
     3841 tagaaaaaga aaaccattta ttccatacta aacaaaagag gtgccacaaa catgttattt
     3901 tgttcatcca aacatgttgt ggcgcctctt ttgtattaca tatgcataat cacaaatttg
     3961 gtacagaaat caatgtccta gactctacaa ctaaaaaagc tatgaaaaaa actatcgtta
     4021 tagatttctt catagctaat cttagtattt tttaaatata cttatttaag ttgtagtgct
     4081 ttattcgcaa tgtcatgacg atagaataaa tggtcacttt gtatttgaga tactttttcg
     4141 tatgcgtctc gctgtgcatc ttgtacattg tcaccttttc cgatggcaag tataactcta
     4201 ccacctgaag taacaaaggt atcaccttgc ttctttaatc cactaacaaa atagttttca
     4261 tttaaatcaa agccacttac tttatgccct ttttcatatg catcaggata tccttttgat
     4321 gccaacatga cccctacaat agattcattt ttccatttga attcagtacg ttttccttca
     4381 tctaaatcaa taatatgctg cattaaatca ctttccatgc gacttaataa tacttgagct
     4441 tcaggatcac caaaacgggc attaaattct attacttttg gaccatcttt agttaaaata
     4501 gcaccaatgt ataatacacc gaagaattga taaccttcat taagcattgc ctttgcaatg
     4561 ggttgtgcaa ttgtttcatt tgtaagtttt aaaacatcgt cactaatatg tggtactgga
     4621 caataagccc ccataccacc agtatttggt ccttcatcat gatcaaatgc gcgtttatga
     4681 tcttgtgcaa tacagtcgaa aggtactgct aaatcaccat taacaaatgt cattagcgag
     4741 aactcttcac cttctaaaaa cgtttcaaat acaacagtac cttcttcttc atcaccatac
     4801 ataatctcaa tagcacttct ggctgcttca atagtatctg caataataac gcctttccca
     4861 gcagctaacc catctttctt gacaacaacg ggcaattcac agttttcaat atatgttaaa
     4921 gcatcctttt ttcgctcaac ttctttataa tcagcagttg gaatattata ttttttcatt
     4981 atctttttag caaataattt tgagccttcg atttgagctg cttgcttatt tggaccaaac
     5041 actttgaaac cattcgctcg taaaatgtct gctaatccat caattagcgg ctgttctgga
     5101 cctataacta cccaatcaac attttgctgt ttagcaaaat ctagtatccc ttgatgatta
     5161 gattctgaaa tttcagtgtg tacttcagct ataggtgtca ttgcctcatt acctggaatg
     5221 acaaactctt gtttaactag attcgattga ttaagtttat atgcaagtgc atgttctcgt
     5281 ccaccagcac caattactaa tacattcatt tatgttacgc ccctttaaaa ttagtgttta
     5341 aaatgtcgag tgcctgtgac taccattgca ataccatgtt tattagccat atcaattgaa
     5401 tcttgatctt taatcgaacc acccggttgg ataattgcct ttataccatg ttgtgctgca
     5461 agttcaactg tatctcccat agggaaaaat ccatcagata ctaacgctac atgatcattg
     5521 atttcaatag ctctctctaa cgcaatttta gcagcaccga cacgattcat ttgtccagca
     5581 cctataccta cagtttgttt attattactt aaaataattg cattactttt tactgatggt
     5641 acaactttcc atcctaataa cattgcttcc cattgttcat cagtaggtgc tacttctgta
     5701 acaactttca tttcttcttt tggcacgaca taattgtctt tatcttgaac taaatatccg
     5761 ccagatactg aaacaaactc ttcttcgtta ctgtctatag tcatatcaat ttctaataat
     5821 cttacattct tcttttgttt taaaatatct aatgcttcat ctgtaaattt tggtgcaata
     5881 atgacttcca aaaagatact atgcaattgc tctgctaact caggtgttac agctcggttt
     5941 aatgcaacaa ttccaccaaa tattgattga ctatccgctt catacgcatg ttgaaatgct
     6001 tgttcaatcg tgtcaccgat accaacacca catggattca tgtgtttaac cgcaactgca
     6061 gcaggtgtat caaacttttt aactaaagct agtgtagcat ctgcatcttt aatattgtta
     6121 tagcttaatt gtttcccatg taattgttta gcacctgcaa tcgtgtgctt agcattcgaa
     6181 gttctcacaa aatacgctga ttgttgtgga ttttctccat atcttaaagt ttctttatcc
     6241 cctttaaaga aacgtacaat cgcttcatca tattctgcag tatgctcaaa aactttaatc
     6301 attaatgatt gtctatatga ctcatctaac gaatcgtttc ttaatcgcgt caatacttca
     6361 tgataatctg ccggatgtac aattgttgtt acatgtttat agtttttagc tgcagcacgt
     6421 aacattgttg gaccaccaat atcaatattt tcaattgctt cgtccatcgt cacatcaggg
     6481 tttgcaacag tttgttggaa tggatataaa ttaactacta ccatatcaat taaatctata
     6541 tgttgttctg ataattcatt taaatgctgc ggtttatttc gatcagctaa aatgccacca
     6601 tgaacagccg gatgtaatgt tttaacacgg ccatccatta tttctggaaa atgtgttaag
     6661 tctgaaacag aacgaactgg tacatttgct tcatctaata tacgtttagt accaccagtt
     6721 gaatataatt cataatttaa ttgcgttaac gctttagcaa actctacaat acctgtttta
     6781 tttgatacgc tcaaaatagc tttcttcatc acatacatac tccttatttt acaattttag
     6841 caataacact tggatataac tcatattcca atttttttac tttctcttct aattgttctt
     6901 ttgaatcgtc cggtctaata tcacattgtc tctgttcaat aatttctccc gtatccatac
     6961 cacaatcaac ataatgtact gtcgaaccag taatagtatc gccactatga tatgcttggc
     7021 ctattgcgtc aatcccctta tatttcggca atagagatgg atgtatattc aatattttac
     7081 cttcaaatga agctaataaa tctggaccaa ttagacgcat gtagccagct aaaataatcc
     7141 actctacctt atctttattt aataatgtta ctaaatgttg ttcatacgct gcttttgaat
     7201 caaattgttt tggttcatta atataaacag gaatatcgtg cttttttgct ctatctatac
     7261 aaaacgcatt ttgatgatcc gtatatagcg ccgtaacttc aatattttca agttttcctg
     7321 attcaacatg ctcaactata ttttcaaagt tacttcctga acctgatgca aaaatcgcaa
     7381 ttttaaccat tgttataccc ccaacaattc aattgcagtt gactcatttt tcacaatatg
     7441 accaatttga taagcttcca cattttgttc tgctaaaatc ttcaaagcgc gtgatgcatc
     7501 tttttcatca acgataaccg tatagccaat acccatgtta aaaatgttat acatttcatt
     7561 tgtgtctata ttgccttgtt gttgtaacca atcaaatatt tttggcgttg gaaatgatgt
     7621 agtatcaatt ctagcagcat atccggctgg caatgcacgt ggaatatttt cataaaaacc
     7681 tccaccagta atatgattca ttgccttaat agaaacttct ttttttaaag caagtacagg
     7741 tttgacatat aatttagttg gttctaaaaa gacatctata aatggacgat tatcgaagtt
     7801 tgatgccaaa tcaatgcctg attcattaat taatttgcgc actaaactgt atccatttga
     7861 atgaatgcca cttgacgcaa gtcctataac aacttgtccc tctttcactt ctgaaccatc
     7921 tacatagtca tccttttcaa ctgctccaac agcaaatccg gctacatcat attcaccttc
     7981 gtgatacatt tcacccattt cagcagtctc tccaccgata agtgcagtat tcgtttcaac
     8041 gcatgcatca ctaatacctt taacaatttg ttcaataact tcaggaacaa ctttatttgt
     8101 agcgatgtaa tctaaaaaat acaatggttc tgcacctgtc gttaaaatat cattaacaca
     8161 cattgcaact gcatcgatac ctatcgaatc atgtttccca tagtcgatag ctagttttaa
     8221 tttcgtacct actccgtctg ttccagaaac taaaactggc gctgtcatat ttaattgtga
     8281 taaatcaaat gtagcaccga atccacctaa accaccgata acttctttac gcatcgtacg
     8341 tttaacatga ctagacattc tttctacagc ttcataacca gcatgaatat ttacaccaga
     8401 ttgttcatat gctttagaca tttaaatttc cctcgctatc aaaaaagtgt ttgtttttag
     8461 aaatatattg cttttgtcga tgacttaaat gtgctttata atttgcttca taatcatata
     8521 accctgcagg ataatctcca gtgaaacttt ctacacataa gccactatat ggcgcgtcat
     8581 aatctaaacc aattgattca attaacccat ctacagatag atatgctaat gaatcagcgc
     8641 caatataatc tttaatttct tcaggtgatt tgcttgcaga aattaattct gccgtagttg
     8701 aaacatcgat tccataaaaa cttgggaaca taaattccgg tgatgctata cgcacatgca
     8761 ctttatttgc accagaatct tttaacattt tcacaatgcg tcgaattgtc gtaccgcgaa
     8821 caatggaatc atcaacaaga atgatatttt tcccatctac tatatctttt accgcagata
     8881 acttcactct cacaccttgc tcacgtaatt cttgagttgg ttgaataaat gttcttgcaa
     8941 catattgatt tttaactagt cccatttcat atggcaaacc tatttcttca gcataaccac
     9001 tcgcagctga tagcgatgaa ttgggtacac cgatgaccat atcagcattt acagggcttt
     9061 cttgggctaa ttttttacca gaagctttac gtactgcatg gacattttta ccagctattg
     9121 ttgagtctgg tctagcaaaa taaatatatt ccatcgcaga aattgcagtt gtcgtatgat
     9181 gtgtataaga tttaactgta atacctttat cgttaatcac gacatattca cctgcatgaa
     9241 tatcttgaac aaattctgca cctaacacat ctattgcaca tgtttcactt gcaaggatgt
     9301 atgtaccatc tttcatttta cctacaacaa gtggtctgat agcatttgga tctactgcgc
     9361 catataacgc atctttagtt aatatcgcaa atgtaaaacc gcctttaact tttcgcaaac
     9421 tttctttcaa tgcttcctca aaagtaggag ctttacttcg acgtatcaaa tgcataatga
     9481 cttcagtatc tgaagacgaa tggaagatag caccttgttt ttctaaattc tgacgcaatg
     9541 atttagcatt aatgaggtta ccattatgac aaatacctac actcatatca taaaagtgat
     9601 acagaaacgg ttgaatattt tcaatacctt tattacctga agtagcataa cggacgtgac
     9661 caattgcatg ttgatatcct tttaatcgtt ccatttgatc atcgttaatc gcttcagtta
     9721 gtaagcctaa tcctcgctcg ccttttaatt cattttgatc agaaacaact atacctgcac
     9781 cttcttgacc acgatgttgc aaactatgaa gtcccatata tgttagttgc gctgcttcag
     9841 gatgattcca aataccaaac acgccacatt cttcgtttaa tcctgagtag ttaaacattg
     9901 agcaattgcc ccttcccata tttgtttaat atctgaaaca ttttcactaa tctctgtata
     9961 tggtgttgtt actttgaaat tatcactatc tgttaaaagt ccaatttcta ttgcattatc
    10021 aatatttaaa gttttacctg atttaacaga aacaacatat cggccttgtg tctcactaaa
    10081 caattgtgca tttgttatat ctattgaaga ttttaatcct aaaccgtaat gcgcacttag
    10141 tttagctaag gtaatcagta agccaccttt accaactgtt tgaacatgtg ataatagtcc
    10201 ttcacgaata gcggtcttga ttgattcacc tttttcaact tctgaactca aatctaatga
    10261 ctcaaattca tgattaactt tgccataaat taacttttca agttgactac caccaaagtc
    10321 gtccttagta tcaccgatta aatataattt atctccaact tgaggttcaa aatcatttaa
    10381 ataatttaca ttttcaatca aacctaccat tccaacaact ggtgttggga aaatagaagt
    10441 acctttcgtt tcgttatata aagatacatt tccagaaact actggtgtct taagaatgtc
    10501 gcatgcttct gccatacctt tcgttgaatc tatcaactgt tgatagattt ctttcttttc
    10561 aggagaacca taatttaaac aatctgtcat tgctaatggt gttgcaccca cggcaattaa
    10621 atttcgataa gcttcagcta ctaccatctt tccaccttca tatggattgt tatatacata
    10681 acgcgcttca ccatcaattg ttgaagcaat tgccttattt gtgccttcca cacgtactac
    10741 cgatgcttga agtcctggct taattatcgt attggcacca acttgttggt cgtattgatc
    10801 atataaatag tgtttagatg ctatagtcgg atgcttaagt aatttaaaga aagtatcttt
    10861 aacatcgatg tgtgtataat catttttaga agtattataa tctttttctt ctccttctaa
    10921 aatatataca ggtgcttcat cagctagtgg ttcaactgga atgtcagcat aaacttcatc
    10981 atcatatgtt aaaacaaaac gatttgtatc tgtaacttca cctataacag cactatccaa
    11041 ttcgtgctta tcaaataaat ctaagaattt ttgttcatta cctttttcaa caactagtaa
    11101 catacgttct tgagtttctg aaagcatcat ttcataagga gaaatacctg gctcacgtgt
    11161 tggcacttgt tctaatctca aatgtaaccc actaccacct tttgccgcca tttcagacga
    11221 tgaagatgtt aaaccagcag cacccatatc ttgaatacca actaattcat caaatgtaat
    11281 tgcttcaagt gttgcttcca ttaatttttt acctacaaat ggatcaccga tttgtacaga
    11341 aggtcgttta ctttcgcttt cttccgtcaa ttcttcagat gcaaaagtag caccatgaat
    11401 accatctcga ccagttttca aaccaacata aatgaccgaa ttacctacac cttttgctgt
    11461 gcctttttga atcatgtcgt gattgataac accaacacac attgcattaa caagtggatt
    11521 gccatcataa cgttcatcaa attcgatttc accagcagtt gttggaatac caatgcagtt
    11581 accataacct ccgataccct ttacaacccc tttaagtaat ctttggtttt gtttattatc
    11641 taattctcca aatctaagac tgtttaacaa attaataggt ctagccccaa tagaaacaat
    11701 gtcacgaatg attccaccaa cgcctgtagc agccccttga tatggttcaa ttgctgatgg
    11761 atgattgtga gactctactt taaatactac ggcttgatta tcacctatat cgactacccc
    11821 tgcaccttca cctggaccca taagcacatg ctcacctgac gtaggaaatt gctttaaaaa
    11881 cggtttagaa tgtttataag agcaatgttc actccacata acagaaaaga tacctgtttc
    11941 tgtaaagtta ggttgtctgc ctaaaatatc gcaaactttt tcatattctt gatcacttaa
    12001 tcccatatct tgatatactt tttcaagttt aatttcttca acgcttggtt cgataaattt
    12061 agacatgttg ttccctccaa ctttttacca tcgcttcaaa taatttcaca ccactatcag
    12121 tacctaacaa cgtttctaaa gctctttcag gatgtggcat catgccacat acattgcctt
    12181 tttcgttaac aattcctgca atatcatcat atgaaccgtt cggattattc acatatttca
    12241 gaataatttg attgttagct tttaattgtt gatatatttc atcagtacaa taataatgac
    12301 cttcaccgtg agctacagga tatataactt tttcaccttg ctcataaaga tttgtaaatg
    12361 ccgtttgatt attcactatt tctaactctt catttctact aataaataaa tgtgaatcgt
    12421 tatgcaataa tgcaccaggt aataagccta tttcagttaa aatttgaaac ccattacaaa
    12481 cacctaatac tggcttacct tcagttgcaa gacgtttaac ttccgaaata atcggcgcta
    12541 cactagccat tgccccagat cttaagtaat ccccgaatga aaatccacca ggaataagta
    12601 cgccatcaaa tccacttagt gatgtttctc tataatctac atattccgct tcaacaccac
    12661 ttttaatagc agcattaaac atgtctctat cacaattcga acctggaaaa acaagaaccg
    12721 caaatttcat tttatgcatt ctccttttca tcatctaaca ctttatagct atattcttca
    12781 atcactgtat ttgcaaacaa tttttcactt agagttgtaa taatgttgtg taccttttca
    12841 tcactaacct catccactgt catatataat acttttccta cacgaatatc attcacttgt
    12901 gcataaccta agtcatgtac agctcgagta agcgtttgtc cttgcgtatc taatacttgt
    12961 ggttgtaatg tgatatgtag ttcaattgtt ttcattattt taaatcctcc aatttgttta
    13021 aaaatatttg atatgtttca atcagtgatc cagtgttatt tctatataca tctttatcaa
    13081 agtttgcatt ggtagcttta tcccaaattc gacatgtatc tggagatatt tcatccgcta
    13141 acaaaatttg accagtctca gtctttccaa attcgatttt aaaatctact aattttaaat
    13201 tcatagcatc cattaattgt ttcaacacat tattaatctt taatgctttg gattttagta
    13261 tttcaatatc ttcatctgat gctatattga gcaatttaac atggtcatcc gttatcaacg
    13321 gatcatttaa cgcatcattt ttatagaaaa attctacaag tggttctcta aaaacttcac
    13381 cattttcaaa acctaaacgc tttgtaatag atccactagc aatattacga acaactactt
    13441 ctaatggaat tattttcaca ggcttaacta attgttctgt ttcagataat tgtttaataa
    13501 agtgactttc tattc
//
LOCUS       DANBWH010000060        13434 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.062, whole genome shotgun sequence.
ACCESSION   DANBWH010000060 DANBWH010000000
VERSION     DANBWH010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13434
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.062"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            116..1411
                     /gene="purB"
                     /locus_tag="PPY92_001187"
     CDS             116..1411
                     /gene="purB"
                     /locus_tag="PPY92_001187"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDJ2625200.1"
                     /translation="MIERYSREEMSNIWTDQNRYEAWLEVEILACEAWSELGHIPKAD
                     VQKIRQNAKVNVERAQEIEQETRHDVVAFTRQVSETLGEERKWVHYGLTSTDVVDTAL
                     SFVIKQANDIIEKDLERFIDVLAEKAKNYKYTLMMGRTHGVHAEPTTFGVKMALWYTE
                     MQRNLQRFKQVREEIEVGKMSGAVGTFANIPPEIESYVCKHLGIGTAPVSTQTLQRDR
                     HAYYIATLALIATSLEKFAVEIRNLQKTETREVEEAFAKGQKGSSAMPHKRNPIGSEN
                     ITGISRVIRGYITTAYENVPLWHERDISHSSAERIMLPDVTIALDYALNRFTNIVDRL
                     TVFEDNMRNNIDKTFGLIFSQRVLLALINKGMVREEAYDKVQPKAMISWETKTPFREL
                     IEQDESITSVLTKEELDECFDPKHHLNQVDTIFERAGLA"
     gene            1520..1822
                     /locus_tag="PPY92_001188"
     CDS             1520..1822
                     /locus_tag="PPY92_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625201.1"
                     /translation="MQIEKLRGAALDELFDAILTLENREECYQFFDDLCTVNEIQSLS
                     QRLQVAKMIKQGYTYATIEQESGASTATISRVKRSLQWGNDAYTMILDRMNIETNE"
     gene            1994..2686
                     /locus_tag="PPY92_001189"
     CDS             1994..2686
                     /locus_tag="PPY92_001189"
                     /EC_number="2.5.1.n9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenylglyceryl phosphate synthase"
                     /protein_id="HDJ2625202.1"
                     /translation="MYDIKKWRHIFKLDPAKHISDDDLDAICMSQTDAIMIGGTDDVT
                     EDNVIHLMSRIRRYPLPLVLEISNIESVMPGFDFYFVPTVLNSTDVAFHNGTLLEALK
                     TYGHSIDFEEVIFEGYVVCNADSKVAKHTKANTDLTTEDLEAYAQMVNHMYRLPVMYI
                     EYSGIYGDVSKVQAVSEHLTETQLFYGGGISSEQQATEMAAIADTIIVGDIIYKDIKK
                     ALKTVKIKESSK"
     gene            2683..4875
                     /gene="pcrA"
                     /locus_tag="PPY92_001190"
     CDS             2683..4875
                     /gene="pcrA"
                     /locus_tag="PPY92_001190"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HDJ2625203.1"
                     /translation="MNALLNHMNTEQSEAVKTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     LDEKDVSPYNVLAITFTNKAAREMKERVQKLVGDQAEVIWMSTFHSMCVRILRRDADR
                     IGIERNFTIIDPTDQKSVIKDVLKNENIDSKKFEPRMFIGAISNLKNELKTPADAQKE
                     ATDYHSQMVATVYSGYQRQLSRNEALDFDDLIMTTINLFERVPEVLEYYQNKFQYIHV
                     DEYQDTNKAQYTLVKLLASKFKNLCVVGDSDQSIYGWRGADIQNILSFEKDYPEANTI
                     FLEQNYRSTKTILNAANEVIKNNSERKPKGLWTANTNGEKIHYYEAMTERDEAEFVIR
                     EIMKHQRNGKKYQDMAILYRTNAQSRVLEETFMKSNMPYTMVGGQKFYDRKEIKDLLS
                     YLRIIANSNDDISLQRIINVPKRGVGPSSVEKVQNYALQNNISMFDALGEADFIGLSK
                     KVTQECLNFYELIQSLIKEQEFLEIHEIVDEVLQKSGYREMLERENTLESRSRLENID
                     EFMSVPKDYEENTPLEEQSLINFLTDLSLVADIDEADTENGVTLMTMHSAKGLEFPIV
                     FIMGMEESLFPHIRAIKSEDDHEMQEERRICYVAITRAEEVLYITHATSRMLFGRPQS
                     NMPSRFLKEIPESLLENHSSGKRQTIQPKAKPFAKRGFSQRTTSTKKQVSSSDWNVGD
                     KVMHKAWGEGMVSNVNEKNGSIELDIIFKSQGPKRLLAQFAPIEKKED"
     gene            4879..6882
                     /gene="ligA"
                     /locus_tag="PPY92_001191"
     CDS             4879..6882
                     /gene="ligA"
                     /locus_tag="PPY92_001191"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDJ2625204.1"
                     /translation="MADLSSRVNELHDLLNQYSYEYYVEDNPSVPDSEYDKLLHELIK
                     IEEEHPEYKTVDSPTVRVGGEAQASFKKVNHDTPMLSLGNAFNEDDLRKFDQRIREQI
                     GNVEYMCELKIDGLAVSLKYVDGYFVQGLTRGDGTTGEDITENLKTIHAIPLKMKEPL
                     NVEVRGEAYMPRRSFLRLNEEKEKNDEQLFANPRNAAAGSLRQLDSKLTAKRKLSVFI
                     YSVNDFTDFNARSQSEALDELDKLGFTTNKNRARVNNIDGVLEYIEKWTSQRESLPYD
                     IDGIVIKVNDLDQQDEMGFTQKSPRWAIAYKFPAEEVVTKLLDIELSIGRTGVVTPTA
                     ILEPVKVAGTTVSRASLHNEDLIHDRDIRIGDSVVVKKAGDIIPEVVRSIPERRPEDA
                     VTYHMPTHCPSCGHELVRIEGEVALRCINPKCQAQLVEGLIHFVSRQAMNIDGLGTKI
                     IQQLYQSELIKDVADIFYLTEEDLLPLDRMGQKKVDNLLAAIQQAKDNSLENLLFGLG
                     IRHLGVKASQVLAEKYETIDRLLTVTEAELVEIHDIGDKVAQSVVTYLENEDIRALIQ
                     KLKDKHVNMIYKGIKTSDIEGHPEFSGKTIVLTGKLHQMTRNEASKWLASQGAKVTSS
                     VTKNTDVVIAGEDAGSKLTKAQSLGIEIWTEQQFVDKQNELNS"
     gene            6895..8094
                     /locus_tag="PPY92_001192"
     CDS             6895..8094
                     /locus_tag="PPY92_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CamS family sex pheromone protein"
                     /protein_id="HDJ2625205.1"
                     /translation="MKRTLVLLITAIFILAACGNHKDDQAGKDNQKHNNSSNQVKEIA
                     TDKNVQGDNYRTLLPFKESQARGLLQDNMANSYNGGDFEDGLLNLSKEVFPTDKYLYQ
                     DGQFLDKKTINAYLNPKYTKREIDKMSEKDKKDKKANENLGLNPSHEGETNPEKIAEK
                     SPAYLSNILEQDFYGGGDTKGKNIKGMTIGLAMNSVYYYKKEKDGPTFSKKLDDSEVK
                     KQGKQMASEILSRLRENDDLKDIPIHFAIYKQSSEDSITPGEFITQATAEKSQTKLNE
                     WHNINEKSALLPSSTAADYDENLNNNFKQFNDNLQSYFSNFTQAVGKVKFVDKKPQRL
                     VVDLPIDYYGQAETIGITQYVTEQANKYFDKIDNYEIRIKDGNQPRALISKTKDDKEP
                     QVHIYSN"
     gene            complement(8183..9721)
                     /gene="putP"
                     /locus_tag="PPY92_001193"
     CDS             complement(8183..9721)
                     /gene="putP"
                     /locus_tag="PPY92_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HDJ2625206.1"
                     /translation="MLTMGTALSQQVDANWQTYIMIAVYFLILIVIGFYGYKQATGNL
                     SEYMLGGRSIGPYITALSAGASDMSGWMIMGLPGSVYSTGLSAMWITIGLTLGAYINY
                     FVVAPRLRVYTELAGDAITLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSG
                     GKLFESAFGLDYHFGLILVAFIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAM
                     MNLNGWGTFHDVAAMKPTNLNLFKGLSFIGIISLFSWGLGYFGQPHIIVRFMSIKSHK
                     MLPKARRLGISWMAVGLLGAVAVGLTGIAFVPAYHIKLEDPETLFIVMSQVLFHPLVG
                     GFLLAAILAAIMSTISSQLLVTSSSLTEDFYKLIRGEEKAKTHQKEFVMIGRLSVLVV
                     AIVAIAIAWNPNDTILNLVGNAWAGFGASFSPLVLFALYWKGLTRAGAVSGMVSGALV
                     VIVWIAWIKPLAHINEIFGLYEIIPGFIVSVIVTYVVSKLTKKPGAFVETDLNKVRDI
                     VREK"
     gene            10088..10390
                     /gene="gatC"
                     /locus_tag="PPY92_001194"
     CDS             10088..10390
                     /gene="gatC"
                     /locus_tag="PPY92_001194"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HDJ2625207.1"
                     /translation="MTKVTREEVEHIANLARLQISPEETEEMANTLESILDFAKQNDS
                     ADTEGVEPTYHVLDLQNVLREDKAIKGIPQELALKNAKETEDGQFKVPTIMNEEDA"
     gene            10392..11849
                     /gene="gatA"
                     /locus_tag="PPY92_001195"
     CDS             10392..11849
                     /gene="gatA"
                     /locus_tag="PPY92_001195"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HDJ2625208.1"
                     /translation="MSIRYESVENLLTLIKDKKIKPSDVVKDIYDAIEETDPTIKSFL
                     ALDKENAIKKAQELDELQAKDQMDGKLFGIPMGIKDNIITNGLETTCASKMLEGFVPI
                     YESTVMEKLHKENAVLIGKLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAA
                     AVAAGLVPLSLGSDTGGSIRQPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTR
                     NVKDNAIVLEAISGADVNDSTSAPVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDV
                     KEAVQNAVETLKSLGAVVEEVSLPNTKFGIPSYYVIASSEASSNLSRFDGIRYGYHSK
                     EAHSLEELYKMSRSEGFGKEVKRRIFLGTFALSSGYYDAYYKKSQKVRTLIKNDFDKV
                     FENYDVVVGPTAPTTAFNLGEEIDDPLTMYANDLLTTPVNLAGLPGISVPCGQSNGRP
                     IGLQFIGKPFDEKTLYRVAYQYETQYNLHDVYEKL"
     gene            11862..13289
                     /gene="gatB"
                     /locus_tag="PPY92_001196"
     CDS             11862..13289
                     /gene="gatB"
                     /locus_tag="PPY92_001196"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HDJ2625209.1"
                     /translation="MHFETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAY
                     PGVLPVVNKRAVDWAMRAAMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGEN
                     GYIDIEVDGETKRIGITRLHMEEDAGKSTHKGEYSLVDLNRQGTPLIEIVSEPDIRSP
                     KEAYAYLEKLRSIIQYTGVSDVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFN
                     YVRKGLEYEEKRQEEELLNGGEIGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIV
                     PLYIDDAWKERVRQTIPELPDERKAKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHG
                     ADVKLTSNWLMGGVNEYLNKNQVELLDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPE
                     LAAKGGNAKQIMEDNGLVQISDEATLLKFVNEALDNNEQSVEDYKNGKGKAMGFLVGQ
                     IMKASKGQANPQLVNQLLKQELDKR"
     gene            13329..>13434
                     /locus_tag="PPY92_001197"
     CDS             13329..>13434
                     /locus_tag="PPY92_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625210.1"
                     /translation="MIADLDAIEGLFISIEVILLLTLFIVHVNGKNINF"
BASE COUNT         5032 a         1990 c         2504 g         3908 t
ORIGIN      
        1 taaccaattc tgttaaactg tgaagagtag agttaaaaac gaactttttg tttattttta
       61 ttaaaaatgt tctgtttttg aaaattcgag tatacatacg ataggagtta aaaaaatgat
      121 tgaacgctat tctagagaag aaatgtctaa tatttggact gatcaaaatc gctatgaagc
      181 atggttagaa gtggaaattt tagcatgtga agcatggagt gaattaggac atataccgaa
      241 agctgacgta caaaaaattc gtcaaaacgc aaaggtaaat gtcgaacgtg cacaagaaat
      301 tgaacaagaa acgcgccatg atgttgtagc ctttactaga caagtttctg aaacgctagg
      361 tgaagaacgt aagtgggtac attatggttt aacttctact gatgttgtag atacagcttt
      421 aagtttcgtt attaaacaag caaatgacat tattgaaaaa gatttagaaa gatttatcga
      481 cgttttagct gaaaaagcaa aaaattataa atatacattg atgatgggac gtacacatgg
      541 agtgcatgca gaaccaacta catttggtgt caaaatggca ttgtggtaca cggaaatgca
      601 acgtaactta caacgtttca aacaagtaag agaagaaatt gaagtaggga aaatgagtgg
      661 tgcagtaggt acttttgcta acattcctcc tgaaatagaa agttatgtgt gtaaacattt
      721 aggtattggc acagcgcctg tatcaacgca aacattgcaa cgcgatcgac atgcatacta
      781 tattgcgaca ttagcattaa tcgcgacgtc actagaaaaa tttgccgttg aaattcgtaa
      841 ccttcaaaaa accgaaacaa gagaagttga agaagcattt gcaaaaggcc aaaaaggttc
      901 atctgcaatg cctcataaac gaaatccaat tggttctgaa aatatcacag gtatttcaag
      961 agtcattcgt ggttatatta ctacagctta tgagaatgtg ccattatggc atgaaagaga
     1021 tatttctcat tcttctgctg agcgtattat gttaccagat gttacaatag cactagatta
     1081 tgcattgaat cgtttcacaa atattgtgga ccgtttaact gtatttgaag ataatatgcg
     1141 taataacata gataaaacat ttggtctgat tttctcacaa cgtgtcttat tagcattaat
     1201 taataaaggt atggttcgtg aagaagcata cgacaaagta caaccaaaag cgatgatatc
     1261 ttgggaaacg aaaacacctt tccgtgaatt aatcgaacaa gatgagtcta ttacaagtgt
     1321 tttaacaaaa gaagaattag atgaatgttt cgatcctaaa catcatttga atcaagttga
     1381 cactattttc gaacgcgctg gattagcata aaattaaata aagtaacgat tcagatttaa
     1441 taaaatataa atcgctacac tttaatagat taatgcgtta aaatgacata aagaataaaa
     1501 ttataggggt tgtttcgata tgcaaattga aaaattacga ggtgcagcgt tagatgaatt
     1561 gtttgatgca atactaacgt tagaaaatag agaagaatgt taccaatttt tcgatgattt
     1621 gtgtactgta aatgaaattc aatcactgtc tcaaagatta caagttgcta aaatgattaa
     1681 gcaaggttat acctatgcaa cgattgaaca agaatctgga gcatcgactg caacgatttc
     1741 tagagtgaaa cgttcattac aatggggtaa tgatgcttat acaatgattt tagatcgtat
     1801 gaatattgaa acaaatgaat aataaatgaa cgataaacaa tggttatcta tctgcactaa
     1861 taaggtagat aatcattgtt ttttcacgaa aaaatttaca gagtaaaaga acttaaaatt
     1921 catattaagt ctttagaact cgacacttaa aaatgctata atcatatgta tgttaaaaaa
     1981 ggagtttcgg aaaatgtatg acattaaaaa atggcgccat atttttaaat tagacccagc
     2041 taaacatatt tcagatgatg atttagatgc gatttgtatg tctcaaacag atgcaattat
     2101 gattggtgga actgatgacg ttactgaaga taatgtcatt catttaatga gcagaataag
     2161 aagataccca ttgcctttag tacttgaaat atcaaatatc gaaagtgtaa tgcctggttt
     2221 tgatttttat tttgtaccta cagtactgaa cagtacagat gttgcatttc acaatggtac
     2281 attattagaa gcgcttaaaa catatggaca tagtatagat tttgaagaag taatatttga
     2341 agggtatgtc gtgtgcaatg ctgatagcaa agtggcaaaa cataccaaag caaatacaga
     2401 tttaacaaca gaagatttag aagcatatgc ccaaatggtc aatcatatgt atcgattacc
     2461 ggttatgtat atagagtata gtggcattta tggcgacgta tcaaaggttc aagctgtctc
     2521 agaacatcta acagaaacgc aactttttta tggtggcggt atttcctcag aacaacaagc
     2581 gacagagatg gcagctattg cagatacaat tatcgtcggt gatattattt ataaagatat
     2641 taaaaaagct ttaaaaacag taaaaataaa ggagtctagt aaatgaatgc gttattaaat
     2701 catatgaata cagagcaaag tgaagctgta aagacaacag aaggaccatt gttaattatg
     2761 gcaggtgctg gttcagggaa gacacgtgtt ttaacacata gaattgctta tttattagac
     2821 gaaaaagatg tctcaccata caatgttttg gctattactt ttacaaataa agctgcaaga
     2881 gaaatgaaag aacgtgttca aaaattagta ggtgatcaag cagaagttat ttggatgtca
     2941 acattccact caatgtgtgt tcgtatttta cgtcgtgatg cagatcgaat aggtatagaa
     3001 cgcaatttta cgataattga tcctacagac caaaaatctg ttattaaaga cgtcttaaaa
     3061 aatgaaaata ttgatagtaa aaagtttgaa cctcgtatgt ttatcggtgc gatcagtaat
     3121 ttgaaaaatg aacttaaaac acctgcagat gctcaaaaag aagccacaga ttatcactcg
     3181 caaatggtag caacggttta tagtggatat caacgccaat tgtcgcgtaa tgaagcgtta
     3241 gattttgatg accttattat gacaacgatt aacttatttg agcgtgtacc agaagttcta
     3301 gaatattatc agaacaaatt ccaatatatt catgtagatg agtatcaaga tactaataaa
     3361 gcacaatata cattagttaa attattagca agtaagttta aaaacttatg tgttgtaggt
     3421 gactcagatc agtcaattta tggttggcgt ggtgctgata ttcaaaatat cttatcattt
     3481 gaaaaagact acccagaagc gaatacaatc tttttagagc aaaattatcg ttcgacgaaa
     3541 acgattttaa atgcggctaa cgaagtgatt aaaaataatt ctgaacgtaa gccaaaagga
     3601 ctgtggactg caaatacgaa tggtgagaaa attcattact atgaagcaat gacagaacgt
     3661 gatgaagcgg aatttgtaat acgagaaatt atgaagcatc aacgtaatgg taagaaatat
     3721 caagatatgg caattttata tagaacaaat gcacaatcac gtgtacttga ggaaacattc
     3781 atgaaatcta atatgccata cacaatggtt ggtggccaaa agttctatga ccgtaaagaa
     3841 atcaaagatt tattaagtta tttgcgtata attgccaata gtaatgatga cattagtttg
     3901 caacgtatta ttaatgttcc aaaaagaggt gtaggtcctt catctgttga aaaagttcaa
     3961 aactatgcac ttcaaaacaa tatcagtatg tttgatgcac ttggagaagc tgattttatc
     4021 ggattgtcaa aaaaggtgac acaagagtgt cttaattttt acgagttaat ccaaagcttg
     4081 ataaaagaac aagaattttt agaaattcat gaaatagttg atgaagtatt acaaaagtct
     4141 ggctaccgag aaatgcttga acgtgaaaat acactagaat ctcgaagtag attagaaaac
     4201 atcgatgaat ttatgtcagt accaaaagac tatgaggaaa ataccccatt agaagaacag
     4261 tcattaatta actttttaac ggatttatcg ttagtagctg atattgatga ggcagatact
     4321 gaaaatggcg taacactaat gacgatgcac tcggctaagg gccttgaatt tccaatcgtc
     4381 tttataatgg ggatggaaga atctttattc ccacatatta gagcgattaa gagtgaagat
     4441 gatcacgaaa tgcaagaaga acgtcgtatt tgttatgtag caattacaag ggctgaagag
     4501 gtgttatata tcactcatgc gacatcaaga atgttatttg gtcgccctca gtcgaatatg
     4561 ccatctagat ttttaaagga aattccagaa tcactattag aaaatcattc aagtggcaaa
     4621 cgacaaacga tacaacctaa ggcaaaacct tttgctaaac gcggatttag tcaacgaaca
     4681 acgtcaacga aaaaacaagt atcgtcatct gattggaatg taggtgacaa agtgatgcat
     4741 aaagcctggg gagaaggcat ggtgagtaat gtaaacgaga aaaatggctc aatcgaacta
     4801 gatattatct ttaaatcaca agggccaaaa cgtttgttag cgcaatttgc accaattgaa
     4861 aaaaaggagg attaagggat ggctgattta tcgtctcgtg tgaacgagtt acatgattta
     4921 ttaaatcaat acagttatga atactatgta gaggataatc catctgtacc agatagtgaa
     4981 tatgacaaat tacttcatga actgattaaa atagaagagg agcatcctga gtataagact
     5041 gtagattctc caacagttag agttggcggt gaagcccaag cctctttcaa aaaagtcaac
     5101 catgacacgc caatgttaag tttagggaat gcatttaatg aggatgattt gagaaaattc
     5161 gaccaacgca tacgtgaaca aattggcaac gttgaatata tgtgcgaatt aaaaattgat
     5221 ggcttagcag tatcattgaa atatgttgat ggatacttcg ttcaaggttt aacacgtggt
     5281 gatggaacaa caggtgaaga tattaccgaa aatttaaaaa caattcatgc gatacctttg
     5341 aaaatgaaag aaccattaaa tgtagaagtt cgtggtgaag catatatgcc gagacgttca
     5401 tttttacgat taaatgaaga aaaagaaaaa aatgatgagc agttatttgc aaatccaaga
     5461 aacgctgctg cgggatcatt aagacagtta gattctaaat taacggcaaa acgaaagcta
     5521 agcgtattta tatatagtgt caatgatttc actgatttca atgcgcgttc gcaaagtgaa
     5581 gcattagatg agttagataa attaggtttt acaacgaata aaaatagagc gcgtgtaaat
     5641 aatatcgatg gtgttttaga gtatattgaa aaatggacaa gccaaagaga gtcattacct
     5701 tatgatattg atgggattgt tattaaggtt aatgatttag atcaacagga tgagatggga
     5761 ttcacacaaa aatctcctag atgggccatt gcttataaat ttccagctga ggaagtagta
     5821 actaaattat tagatattga attaagtatt ggacgaacag gtgtagtcac acctactgct
     5881 attttagaac cagtaaaagt agctggtaca actgtatcaa gagcatcttt gcacaatgag
     5941 gatttaattc atgacagaga tattcgaatt ggtgatagtg ttgtagtgaa aaaagcaggt
     6001 gacatcatac ctgaagttgt acgtagtatt ccagaacgta gacctgagga tgctgtcaca
     6061 tatcatatgc caacccattg tccaagttgt ggacatgaat tagtacgtat tgaaggcgaa
     6121 gtagcacttc gttgcattaa tccaaaatgc caagcacaac ttgttgaagg attaattcac
     6181 tttgtatcaa gacaagccat gaatattgat ggtttaggca ctaaaattat tcaacagctt
     6241 tatcaaagcg aattaattaa agatgttgct gatattttct atttaacaga agaagattta
     6301 ttacctttag atagaatggg gcagaaaaaa gttgataatt tattagctgc cattcaacaa
     6361 gctaaggaca actctttaga aaatttatta tttggtctag gtattaggca tttaggtgtt
     6421 aaagcgagcc aagtgttagc agaaaaatat gaaacgatag atcgattact aacggtaact
     6481 gaagcggaat tagtagaaat tcatgatata ggtgataaag tagcacaatc tgtagttact
     6541 tatttagaaa atgaagatat tcgtgcttta attcaaaaat taaaagataa acatgttaat
     6601 atgatttata aaggtatcaa aacatcagat attgaaggac atcctgaatt tagtggtaaa
     6661 acgatagtac tgactggtaa gctacatcaa atgacacgca atgaagcatc taaatggctt
     6721 gcatcacaag gtgctaaagt tacaagtagc gttactaaaa atacagatgt cgttattgct
     6781 ggtgaagatg caggttcaaa attaacaaaa gcacaaagtt taggtattga aatttggaca
     6841 gagcaacaat ttgtagataa gcaaaatgaa ttaaatagtt agaggggtat gtcgatgaag
     6901 cgtacattag tattattgat tacagctatc tttatactcg ctgcttgtgg taaccataag
     6961 gatgaccagg ctggaaaaga taatcaaaaa cataacaata gttcaaatca agtaaaagaa
     7021 attgctacgg ataaaaatgt acaaggtgat aactatcgta cattgttacc atttaaagaa
     7081 agccaggcaa gaggactttt acaagataac atggcaaata gttataatgg cggcgacttt
     7141 gaagatggtt tattgaactt aagtaaagaa gtatttccaa cagataaata tttgtatcaa
     7201 gatggtcaat ttttggacaa gaaaacaatt aatgcctatt taaatcctaa gtatacaaaa
     7261 cgtgaaatcg ataagatgtc tgaaaaagat aaaaaagaca agaaagcgaa tgaaaattta
     7321 ggacttaatc catcacacga aggtgaaaca aatcctgaaa agattgcaga aaaatcacca
     7381 gcctatttat ctaacatttt agagcaagat ttttatggtg gtggagatac aaaaggtaag
     7441 aatattaaag gtatgacgat tggtttagct atgaatagtg tttattacta taaaaaagaa
     7501 aaagatggac cgacttttag taaaaaacta gatgatagcg aagttaaaaa gcaaggtaaa
     7561 caaatggcta gtgagatatt atcaaggtta cgtgaaaatg atgatttaaa agatatacca
     7621 attcattttg caatttataa gcaatcaagt gaagattcaa tcacaccagg tgaatttatc
     7681 actcaagcga ctgcagaaaa gagtcaaaca aagcttaatg aatggcataa tatcaatgaa
     7741 aaatcagctt tattaccttc ttcaacagca gcagattatg atgaaaattt aaataataat
     7801 ttcaagcaat ttaatgataa tttgcaatca tatttttcta atttcacaca agcagtaggg
     7861 aaagttaaat ttgttgataa aaagccacaa cgattagtag tagatttacc aatcgattac
     7921 tatggacaag ctgaaacaat tggtattaca cagtacgtta ctgaacaagc gaataaatat
     7981 ttcgataaaa tcgataacta tgaaattcgt attaaagatg gtaaccaacc acgtgcttta
     8041 attagtaaga caaaagatga caaagaaccg caagttcata tttacagtaa ttaattgtaa
     8101 attaaagata ataaaaaggt accaatgtta gtcataattt tgattaacat tagtaccttt
     8161 ttaatttgtt aagacttatg aattattttt ctctaacgat gtcacgaact ttgtttaagt
     8221 cagtttcaac aaatgcacca ggttttttag taagtttact tacaacatat gtaacgatta
     8281 cacttacaat aaatccagga ataatttcat ataagccgaa tatttcgttg atatgtgcca
     8341 atggtttaat ccacgcaatc caaacgataa cgactaaggc accggaaacc attccactta
     8401 cagcaccggc acgtgtcaaa cctttccagt aaagtgcaaa tagcacaagt ggactgaacg
     8461 atgcaccaaa tccggcccaa gcgttaccta ctaagtttag aattgtgtcg tttggattcc
     8521 atgcaatcgc gatggcaaca attgctacaa ctaatacaga taatcttcca atcataacaa
     8581 attctttttg gtgcgtttta gctttttctt caccacgaat taatttataa aagtcttccg
     8641 ttagtgaact agatgttaca agtaattgtg aagaaatcgt gctcataatt gcagctagaa
     8701 tcgcagcaag taagaaacca cctacaagag gatggaagag tacttgactc atcacgatga
     8761 ataatgtctc aggatcttct agtttaatat gataagcagg tacgaatgca atacctgtta
     8821 aaccaacagc cacagcgcct aataaaccaa cagccatcca gctaatacct aaacgtctag
     8881 ctttaggtag catcttgtgt gatttaatag acataaacct tacaatgata tgaggttgac
     8941 cgaaataacc taatccccat gaaaatagag agataattcc tataaatgat aaccctttaa
     9001 ataaatttaa atttgtaggt ttcatagctg ctacatcatg aaacgttccc cagccgttta
     9061 aattcatcat agcaacaata gggaccataa ccatcgcaat taacataatg acaccttgga
     9121 agaaatctgt aattgataca gctaaatatc caccaaagaa agtatagaaa atgacaatga
     9181 aagcaactaa tattaaaccg aaatgataat ctaatccaaa agcactttca aataatttac
     9241 caccagatac gaaaccagaa tgtgtatata atgtaaagaa tactacgata atcaatccag
     9301 aaataatctt taacacatta tttttatcgt ttaaacgatt tttaaagaaa tctggtaatg
     9361 taattgcatc tccagctaat tcggtataaa cacgaagtct aggagcaaca acaaagtaat
     9421 ttatataagc acctaatgtt aaaccgattg taatccacat agctgataga ccagtgctat
     9481 agacagaacc aggtagcccc ataatcatcc atccactcat atctgaagct ccagctgata
     9541 atgcagtaat atacggtcca atactacgtc cacctaacat gtactcgctt aggttaccag
     9601 ttgcttgctt gtaaccgtaa aagccaataa cgattagtat caagaagtag acggcaatca
     9661 taatataagt ttgccaattg gcatctactt gttgacttaa tgctgtcccc attgtaagca
     9721 tgataaacac tccctttatt taatttataa tttagttact tgaaaacatt atacataaag
     9781 ataaatgttt agaaaagtag gaaaattaaa tttattcttt ttgaaatact tgcataccaa
     9841 tataggaagc gctttcgtat tttttggata tgagcaaatt tttataatta aaattgcata
     9901 atcataatat tgaattgggt tgtctaatat taaaagagtg ttgttttcat aaaaaatgat
     9961 ttgataaacg aatgataatg tttatacagt ttatatgaaa ttaaagtagc acctcataaa
    10021 tacttagatt tttaattgga aatttgatac aatttagtga tgaatgactt aaaggaggct
    10081 tttattaatg acaaaagtaa cacgtgaaga agttgagcat atcgcgaatc ttgcaagact
    10141 tcaaatttct cctgaagaaa cggaagaaat ggccaacaca ttagaaagca ttttagattt
    10201 tgcaaaacaa aatgatagcg ctgatacaga aggcgttgaa cctacatatc acgttttaga
    10261 tttacaaaac gttttacgtg aagataaagc aattaaaggt attccgcaag aattagcttt
    10321 gaaaaatgcc aaagaaacag aagatggaca atttaaagtg cctacaatca tgaatgagga
    10381 ggacgcgtaa gatgagcatt cgctacgaat cggttgagaa tttattaact ttaataaaag
    10441 acaaaaaaat caaaccatct gatgttgtta aagatatata tgatgcaatt gaagagactg
    10501 atccaacaat taagtctttt ctagcgctgg ataaagaaaa tgcaatcaaa aaagcgcaag
    10561 aattggatga attacaagca aaagatcaaa tggatggcaa attatttggt attccaatgg
    10621 gtataaaaga taacattatt acaaacggat tagaaacaac atgtgcaagt aaaatgttag
    10681 aaggttttgt gccaatttac gaatctactg taatggaaaa actacataaa gagaatgccg
    10741 ttttaatcgg taaattaaat atggatgagt ttgcaatggg tggttcaaca gaaacatctt
    10801 atttcaaaaa aacagttaac ccatttgacc ataaagcagt accaggtggt tcatcaggtg
    10861 gatctgcagc agcagttgca gctggcttag taccacttag cttaggttca gacacaggtg
    10921 gttcaattag acaaccggct gcatattgtg gcgttgtcgg tatgaaacca acatacggtc
    10981 gtgtatctcg atttggatta gttgcttttg catcttcatt agaccaaatt ggtccattga
    11041 ctcgaaatgt aaaagataat gcaatcgtat tagaagctat ttctggtgca gatgttaatg
    11101 actctacaag tgcaccagtt gatgatgtag actttacatc tgaaattggt aaagatatta
    11161 aaggattaaa agttgcatta cctaaagaat acttaggtga aggtgtagct gatgacgtaa
    11221 aagaagcagt tcaaaacgct gtagaaactt taaaatcttt aggtgctgtc gttgaggaag
    11281 tatcattgcc aaatactaaa tttggtattc catcatatta cgtgattgca tcatcagaag
    11341 cttcgtcaaa cctttctcgt tttgacggaa ttcgttatgg ttatcattct aaagaagctc
    11401 attcattaga agaattatat aaaatgtcaa gatctgaagg tttcggtaaa gaagtaaaac
    11461 gtcgtatttt cttaggtaca tttgcattaa gttcaggtta ctacgatgct tactataaaa
    11521 aatctcaaaa agttagaaca ttgattaaaa atgactttga taaagtattc gaaaattatg
    11581 atgtagtagt tggtccaaca gcgcctacaa ctgcgtttaa tttaggtgaa gaaattgatg
    11641 atccattaac aatgtatgcc aatgatttat taacaacacc agtaaactta gctggattac
    11701 ctggtatttc tgttccttgt ggacaatcaa atggccgacc aatcggttta cagttcattg
    11761 gtaaaccatt cgatgaaaaa acgttatatc gtgtcgctta tcaatatgaa acacaataca
    11821 atttacatga cgtttatgaa aaattataag gagtggaaat catgcatttt gaaacagtta
    11881 taggacttga agttcacgta gagttaaaaa cggactcaaa aatgttttct ccatcaccag
    11941 cgcattttgg agcagaacct aactcaaata caaatgttat cgacttagca tatccaggtg
    12001 tcttaccagt tgttaataag cgtgcagtag actgggcaat gcgtgctgca atggcactaa
    12061 atatggaaat cgcaacagaa tctaagtttg accgtaagaa ctatttctat ccagataatc
    12121 caaaagcata tcaaatttct caatttgatc aaccaattgg tgaaaatgga tatatcgata
    12181 tcgaagtcga cggtgaaaca aaacgaatcg gtattactcg tcttcacatg gaagaagatg
    12241 ctggtaagtc aacacataaa ggtgagtatt cattagttga cttgaaccgt caaggtacac
    12301 cgctaattga aatcgtatct gaaccagata ttcgttcacc taaagaagca tatgcatatt
    12361 tagaaaaatt acgttcaatt attcaataca ctggtgtatc agacgttaag atggaagagg
    12421 gatctttacg ttgtgatgct aacatctctt tgcgtccata tggtcaagaa aaatttggta
    12481 ctaaagccga attgaaaaac ttaaactcat ttaactatgt acgtaaaggt ttagaatatg
    12541 aagaaaaacg ccaagaagaa gaattgttaa atggtggaga aatcggacaa gaaacacgtc
    12601 gatttgatga atctacaggt aaaacaattt taatgcgtgt taaagaaggt tctgatgatt
    12661 accgttactt cccagagcct gacattgtac ctttatatat tgatgatgct tggaaagagc
    12721 gtgttcgtca gacaattcct gaattaccag atgagcgtaa ggctaagtat gtaaatgaat
    12781 taggtttacc tgcatacgat gcacacgtat taacattgac taaagaaatg tcagatttct
    12841 ttgaatcaac aattgaacac ggtgcagatg ttaaattaac atctaactgg ttaatgggtg
    12901 gcgtaaacga atatttaaat aaaaatcaag tagaattatt agatactaaa ttaacaccag
    12961 aaaatttagc aggtatgatt aaacttatcg aagacggaac aatgagcagt aaaattgcga
    13021 agaaagtctt cccagagtta gcagctaaag gtggtaatgc taaacagatt atggaagata
    13081 atggcttagt tcaaatttct gatgaagcaa cacttctaaa atttgtaaat gaagcattag
    13141 acaataacga acaatcagtt gaagattaca aaaatggtaa aggcaaagct atgggcttct
    13201 tagttggtca aattatgaaa gcgtctaaag gtcaagctaa tccacaatta gtaaatcaac
    13261 tattaaaaca agaattagat aaaagataat ttaaatcatc aaactatgaa gatttaaaaa
    13321 ataaaccctt gattgctgac ttagatgcaa tcgagggttt atttatatct atagaagtca
    13381 tattactttt aactttattc attgtacatg ttaatggtaa aaatattaat ttta
//
LOCUS       DANBWH010000061        13067 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.063, whole genome shotgun sequence.
ACCESSION   DANBWH010000061 DANBWH010000000
VERSION     DANBWH010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13067
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.063"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(112..387)
                     /locus_tag="PPY92_001198"
     CDS             complement(112..387)
                     /locus_tag="PPY92_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDJ2625211.1"
                     /translation="MENKFVPGILIGAVIGGAISLADKSTRQALVQSVKDAKNGNRTR
                     KPSKVSKIKDEVLYWKDVVEEIRRNNPELERSLKDAKETFVNRKNQR"
     gene            complement(394..858)
                     /locus_tag="PPY92_001199"
     CDS             complement(394..858)
                     /locus_tag="PPY92_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HDJ2625212.1"
                     /translation="MVDVAFVCLGNICRSPMAEAIMRQRLKDRNIHDIKVHSRGTGSW
                     NLGEPPHEGTQKILNKHNIPFDGMISELFEATDDFDYIVAMDQSNVDNIKSINPNLKG
                     QLFKLLEFSNMEESDVPDPYYTNNFEGVYDMVLSSCDNLIDYIVKDANLKEG"
     gene            1005..1205
                     /locus_tag="PPY92_001200"
     CDS             1005..1205
                     /locus_tag="PPY92_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1128 family protein"
                     /protein_id="HDJ2625213.1"
                     /translation="MTNEEKVLAIREKLNIVNQGLLDPEKYKNANEEELTDIYDFVQS
                     RERLSPSEVTAIADALGQLRHD"
     gene            1208..2464
                     /locus_tag="PPY92_001201"
     CDS             1208..2464
                     /locus_tag="PPY92_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDJ2625214.1"
                     /translation="MGILTNYKEKLQQYAELLVKVGMNVQPKQPVFIRSSVETLELTH
                     LIVEEAYHCGASDVRVVYSDPTLKRLKFENESVEHFANHEIKSYDVEARMDYVKRGAA
                     NLALISEDPDLMDGIDSQKLQAFQQQNARAFKGYMESVQKNQFPWVVAAFPSKAWAKR
                     VYPELSVEEAYIKFIDEIFDIVRIDGNDPVENWRQHIANLSVYAQKLQQKNYHALHYV
                     SEGTDLTVGLAKNHIWEDATSYVNGEEQAFIANIPTEEVFTAPDRNRVDGYVTNKLPL
                     SYNGTIIDQFKLMFKDGEIIDFSAEKGEAVLKDLINTDEGSRRLGEVALVPDDSPISN
                     RNTIFYNTLFDENAACHLAIGSAYAFNIQGGTEMTVEEKIASGLNDSNVHVDFMIGSS
                     DLTIYGIFEDGSKELVFENGNWASTF"
     gene            2554..3084
                     /locus_tag="PPY92_001202"
     CDS             2554..3084
                     /locus_tag="PPY92_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDJ2625215.1"
                     /translation="MTNQDRPMKSMSESKCYKNRQVFPQDTNHHHTMFGGTLMANIDE
                     IAAITAMKHAGAQVVTASTDSVDFLKPIKTGDILQYVAMVSYAGTSSMEVVVQIRIDD
                     VFNNKHDLAALSYLTFVALDDEGKPKHVPGVYPEDDVEKWFYDTAPQRVERRKARRIE
                     SKQTIEYLAQVQHIRD"
     gene            complement(3344..4495)
                     /gene="yfkAB"
                     /locus_tag="PPY92_001203"
     CDS             complement(3344..4495)
                     /gene="yfkAB"
                     /locus_tag="PPY92_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/CxCxxxxC motif protein YfkAB"
                     /protein_id="HDJ2625216.1"
                     /translation="MNSVKLKQPVSIYNDPWESYNDVKEHGQLTLSNIEFTTTNLCNM
                     RCSHCAVGYTLQTVDPEPLDMDLIYRRLDEIPNLRTMSITGGEPMFSKKSIRNVVKPL
                     LKYAHHRGIYTQMNSNLTLPQDRYLDIAEYIDVMHISHNWGTTDEFANVGFGAMKKQP
                     PLKAKLKLYEQMISNARTLSEQGMFVSAETMLNQSTLPHLRKIHQEVVHDMKCSRHEI
                     HPMYPADFASQLNVLTLAEMKKTIHDILDFRDEDIWMLFGTLPVFPCLKDDEDQKLLS
                     RLRNANNVTTRNDPDGRSRLNVNVFTGNVIVTDFGDETGTISNIQKDKLTDVFDKWLS
                     SDLAKSLNCHCSEFSCLGPNVLVKNMYYPNMDFKDNERHMHKQPQIIQF"
     gene            4719..4880
                     /locus_tag="PPY92_001204"
     CDS             4719..4880
                     /locus_tag="PPY92_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625217.1"
                     /translation="MNEQQTIEQIKARLNKFIEDIDHVNPDEVRVEDIDEWIGLLDQL
                     EEKVKLVSK"
     gene            5011..5526
                     /locus_tag="PPY92_001205"
     CDS             5011..5526
                     /locus_tag="PPY92_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDJ2625218.1"
                     /translation="MTKKVAIILANEFEDIEYSSPKEALENAGFNTVVIGDTANSEVV
                     GKHGEKVTVDVGIAEAKPEDYDALLIPGGFSPDHLRGDTEGRYGTFAKYFTKNDVPTF
                     AICHGPQILIDTDDLKGRTLTAVLNVRKDLSNAGAHVVDESVVVDNNIVTSRVPDDLD
                     DFNREIVKQLQ"
     gene            5866..6675
                     /gene="sgtB"
                     /locus_tag="PPY92_001206"
     CDS             5866..6675
                     /gene="sgtB"
                     /locus_tag="PPY92_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional peptidoglycan glycosyltransferase
                     SgtB"
                     /protein_id="HDJ2625219.1"
                     /translation="MKRSDRYSNSNEHFEHMKHEPHYNTYYQPVGKPPKKKKSKRILL
                     KILLTILIIIALFIGIMYFLSTRDNVDELRKIENKSSFVSADNMPEYVKGAFISMEDE
                     RFYNHHGFDLKGTTRALFSTISDRDVQGGSTITQQVVKNYFYDNDRSFTRKVKELFVA
                     HRVEKQYNKNEILSFYLNNIYFGDNQYTLEGAANHYFGTTVNKNSTTMSHITVLQSAI
                     LASKVNAPSVYNINNMSENFTQRVSTNLEKMKQQNYINETQYQQAMSQLNR"
     gene            6935..7753
                     /gene="recX"
                     /locus_tag="PPY92_001207"
     CDS             6935..7753
                     /gene="recX"
                     /locus_tag="PPY92_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDJ2625220.1"
                     /translation="MPKITKIEVQKKNKERFNLFLDEQFEMGIDIDTLVKFNLKKGQQ
                     LEAADMAEIQKYDHYRIGLNKAIQYLSYKKRTEKEVIQYLQKEEISEQAISEVIEYCY
                     REKLIDHQDYAESLKNTMIRTTDKGPKIYQQKLYQLGIEPNIIEMFTELYREQQELDD
                     IIQIAEKISKTKKGPQNKVKEKVMQSLIQKGFEMETIHAVLNEMDFTQDEAVLDDLLQ
                     RDLEKIYNKNRKKYTQQKLISKTIEGLMRKGYKYDKIKAKLEESGIADGTEEIE"
     gene            7731..8045
                     /locus_tag="PPY92_001208"
     CDS             7731..8045
                     /locus_tag="PPY92_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhH family protein"
                     /protein_id="HDJ2625221.1"
                     /translation="MEQKKLSEMSEPELRHEIQLYKEKMRKAEMNGILNEYDVYQSKV
                     IVAESYLVDRKKIEIGKIYKLTDGSNQYFKVERLKGIFAWGFRFNSDEPEEGLPIALL
                     QL"
     gene            8060..9577
                     /locus_tag="PPY92_001209"
     CDS             8060..9577
                     /locus_tag="PPY92_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDJ2625222.1"
                     /translation="MGSSIVLKLLKVTHYYRNKQNKKWYLPFGYDAEDIDLNNISLHI
                     YQGEALGIIGEPESSKALVGQLLAGAIKPDKGKVVCTEDLFYGYIEDQSLIHQTVEAY
                     TAQLVQLFPYEINDHKAEQIIQYAHLGDYKTKPVNHISKAAYAQLLLSIARSSKSNII
                     ILNHVIDYLTPQFMERAIELTNDYIENNLTIVSIGDDIDKISQVSNYIAWFSHGQLRM
                     EGSLKQVIPSFKEHERDRLSLNSKEEIENFDLDWKKNRTRIPEMTYNFKRVERYNHAK
                     PPKFLVRFWTLASGTILGLALMMLLIFNNIGIISITDFTNRATMQNENKDPYGEKLAY
                     GIAFNGSVDMQGDKQVTIPKYSVVTITGENSKNYRVTADNKTYYVSKDKLEYFNPAGL
                     YQTHSFKKLAPYMKSNYSNYYAYFNSQLHKKHSSVIKTLVPDDDNRFVASVTQQPIQL
                     LFNDNNQLYGFVYPIVDKKELKDKFNINNNIWITKVGNGYCIANLKEDKWIYIEL"
     gene            9586..10422
                     /locus_tag="PPY92_001210"
     CDS             9586..10422
                     /locus_tag="PPY92_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625223.1"
                     /translation="MLDNIILYFKNLPHTKRYVTERLKQSWKSFLIVLAACLILIIAS
                     ETLFSFSHLTDVKEVRWLFRIIALIVFAVLMFTIYISYHHYMNDFLVTKLFNISAATP
                     VVIMSILSFIMLVILTMISALVKPVNFETSYIALFYFIVMATIFVGLISVTFGLIRLL
                     TEKINIIFYGVCVLCILLLPIIFIPNPNHVFINHILMLNPMYYIVNGIAQSIIFGISS
                     MENIPYHFYFILFLCLIAAVNFVLARYTTHAIYNKTSKVTQTDNQQDVSNDSTDEADT
                     SS"
     gene            complement(10683..11660)
                     /locus_tag="PPY92_001211"
     CDS             complement(10683..11660)
                     /locus_tag="PPY92_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDJ2625224.1"
                     /translation="MDTATHIAIGVGLTALATQDPAMASTFGATATTLIVGSLIPDGD
                     TVLKLKDNATYISHHRGITHSIPFTILWPILITFLIFTFFSGTNPFHVWMWAQLAVFL
                     HVFVDIFNSYGTQALRPITNKWIQLSVINTFDPIIFTVLCIGIVLWVIGLHPFAVFFP
                     IIALLIIYYMIRFKMRAVIKQQALKAIQQEHHPVKVFVAPTIKFMEWRVAIQTDAHDY
                     VGKAYGRNVVFSDKVERQTLSTDSILWKVKGNKDIRTFLNFSSIYRWQTTTLADGSTE
                     IRLIDLRYLKNDHYSFVAIAHVTNDNVIDHSYIGWVFTEDKLQRKLYAK"
     gene            11812..12849
                     /gene="mutY"
                     /locus_tag="PPY92_001212"
     CDS             11812..12849
                     /gene="mutY"
                     /locus_tag="PPY92_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDJ2625225.1"
                     /translation="MYQQSSFKENLIHWFDENQREMPWRQTTNPYYIWLSEVMLQQTQ
                     VKTVIDYYHRFVERFPTVEVLSQASEDEVLKYWEGLGYYSRARNFHTAIKEVYDKYEG
                     LVPKDPDQFKALKGVGPYTQAAVMSIAYNVPLATVDGNVFRVWSRLNDDYRDIKLQST
                     RKSYEQELLPYVTTEAGTFNQAMMELGALICTPKNPLCLFCPVQENCEAFDKGTFEKL
                     PVKSKNVSKKVIEQSVFLIRNNQGQYLLQKRSEKLLHGMWQFPMFESEHARRKMTEKI
                     GHDIQPVETPIFELKHQFTHLTWKIKVYAASGAINIETLPDDMIWFNLSDRDQYTFPV
                     PMSKIYQFING"
BASE COUNT         4715 a         1840 c         2259 g         4253 t
ORIGIN      
        1 caatactgaa acaatgtagt ttggccaatc attgtattat catttaataa tgataactat
       61 gaaaggcgta caccaaattg ttaagtattg ccttataaaa acaaaatcat cttagcgttg
      121 attttttcta ttaacaaatg tttctttcgc atcctttaat gaacgttcta attcaggatt
      181 attacgacga atttcttcaa caacatcttt ccagtataaa acttcgtctt taatcttgct
      241 gactttagaa ggcttacgag tgcggttacc attttttgca tctttaactg attgaactaa
      301 agcttgacgt gtagatttat cagctaaact aattgcacca ccaattacgg caccaattaa
      361 aataccagga acaaatttat tttccataaa aaactacccc tctttcaaat ttgcatcttt
      421 tacgatgtag tctattaaat tatcacaaga tgataatacc atgtcgtata caccttcaaa
      481 attattcgtg tagtatggat ctggtacatc actctcttcc atattactaa attctaacag
      541 tttgaacaat tgtcccttaa gattaggatt gatagattta atattatcaa cgttactttg
      601 atccatagcc acaatgtaat caaaatcatc tgtcgcttcg aataattcac taatcatgcc
      661 atcaaatgga atattgtgtt tgttgagaat tttttgtgta ccttcatgag gcggctctcc
      721 taaattccag ctaccagtac ctcttgaatg tactttaata tcatgaatat ttctgtcttt
      781 aagtctttgt cgcatgattg cttctgccat tggagaacga catatattgc caagacagac
      841 aaatgctaca tctaccattt tgattcctcc aaactatgta gttatatccc cattttatag
      901 cgactttaaa caataagaaa gcagattata taaaattcta ttaaagttta ttaaattgtg
      961 atactttgat aacataacta ttattagagg tgaacattgt ggctatgaca aatgaagaga
     1021 aagtattagc tattagagag aagttaaata ttgttaatca aggattatta gatcctgaaa
     1081 aatataaaaa tgcaaatgaa gaagaattaa cagatatata tgattttgtt caatcaagag
     1141 aaagattgtc gccaagtgaa gtgacagcta ttgctgacgc tttaggacaa ttgcgacacg
     1201 actaggagtg ggaattttga ctaattacaa agaaaagtta caacaatacg ctgaactatt
     1261 agttaaggtt ggtatgaatg ttcaaccaaa acagccagta tttattagat catcagtaga
     1321 aacattagaa ttaacgcatt taattgtaga agaagcatat cattgtggcg cttcagatgt
     1381 acgtgttgta tatagcgatc caaccttaaa acgcttaaaa tttgaaaatg aatctgtgga
     1441 acattttgct aatcatgaga taaaatcata tgatgttgaa gcgagaatgg attacgttaa
     1501 acgtggcgct gctaatttag cacttattag tgaagatcct gatttaatgg atggtatcga
     1561 tagccaaaaa ttacaagcat ttcaacaaca aaacgcacgc gcatttaaag gatatatgga
     1621 aagtgtacaa aaaaatcaat ttccatgggt cgtagctgct tttccatcta aagcttgggc
     1681 gaagagagta tatcctgaac tatctgtaga agaagcatat attaagttta ttgacgagat
     1741 atttgatatt gtgcgaattg atggtaacga tccagttgaa aattggcgtc aacacatcgc
     1801 taatttaagt gtttacgctc aaaagttaca acaaaaaaat taccatgcgt tgcattatgt
     1861 atctgaaggt actgatttaa cagtaggttt agcgaaaaat catatctggg aagatgctac
     1921 aagttatgtt aacggcgagg aacaagcttt tattgctaat ataccaactg aagaagtatt
     1981 tacagcacca gatcgaaata gagtagatgg atatgttact aataaattgc ctttaagtta
     2041 taatggaacg attatcgatc aatttaaatt gatgtttaaa gatggagaaa ttattgattt
     2101 ttcagctgaa aaaggtgaag cggtattgaa agatttaatc aataccgatg aaggttcaag
     2161 aagattaggt gaagtagcgt tagtacctga tgattcaccg atatcaaatc gtaataccat
     2221 tttttacaat actttatttg atgaaaatgc agcatgtcat ttagcgattg gatctgctta
     2281 cgcctttaat attcaaggtg gaacggaaat gactgttgag gaaaagattg caagcggatt
     2341 aaatgattca aacgtacatg tcgatttcat gattggtagt agtgatttga ctatttatgg
     2401 catattcgaa gatggttcaa aagaactagt atttgaaaat ggaaattggg catcaacatt
     2461 ttaataaatg ttattttgag gtgctgagta ggaaatgaaa catgtattta aaggtcaaat
     2521 acgtgattaa agtatagatt gggagataaa ataatgacaa atcaggacag accaatgaaa
     2581 tctatgtcag aatcaaaatg ttataaaaat agacaagttt tccctcaaga tacgaatcac
     2641 catcatacaa tgtttggtgg tacattgatg gctaatattg atgaaattgc agcaatcaca
     2701 gctatgaaac atgctggtgc gcaagtagtt accgcatcta cagactcagt agatttctta
     2761 aagccgatta aaacagggga catattacaa tacgtagcga tggtttcata cgctgggact
     2821 agttcaatgg aagtggtcgt tcaaattaga attgacgatg tatttaataa caagcatgat
     2881 ttagctgcat taagttactt aacatttgtt gcgttagatg atgaaggcaa acctaaacat
     2941 gtacctggtg tatatccaga agatgatgtt gaaaaatggt tttatgatac agcaccacaa
     3001 cgtgttgaac gaagaaaagc acgtcgcatt gaaagtaaac aaacaattga atatttagct
     3061 caagtgcaac atattagaga ctaaaaatat aaagacagat tgttaaggat gaattgaact
     3121 gttatgttcg aatattccac ctacaatctg tctttttgat tatgcacgtt tatgctttta
     3181 gttaaaattt aaccttttta ttatagacta ttagtcctat taaatgacct ttattattaa
     3241 agtagagtac tgccagcaat taattgtttt ttattttata atagcattaa aatatgcgga
     3301 gactacttat cgataaagtg cttctccgca taattaagag tttttaaaat tgtataattt
     3361 gtggttgttt gtgcatatga cgctcattat ctttaaaatc catattcgga tagtacatat
     3421 ttttaacaag aacatttggt cctaaacaac taaactcgga acaatgacaa tttaatgatt
     3481 tagcaagatc agaggataac catttatcaa atacatctgt taatttatct ttttgtatat
     3541 tcgaaattgt acctgtttca tctccgaaat cagttacgat tacattacct gtaaatacat
     3601 tgacatttaa acgactacgg ccatccgggt catttctagt cgttacattg ttagcatttc
     3661 ttaaacgtga tagtaacttt tgatcttcat catcctttaa gcatggaaac acaggcaaag
     3721 taccaaataa catccaaata tcttcatctc tgaaatccaa tatatcatga attgtctttt
     3781 tcatttccgc tagagttaac acatttaatt gacttgcaaa gtcagctgga tacatagggt
     3841 gaatctcgtg tctgctacat ttcatatcat gaacgacttc ttgatgtatt tttcgtaaat
     3901 gtggtagcgt actttgattg agcattgttt ccgcagatac aaacattcct tgttctgata
     3961 atgtacgtgc attcgaaatc atttgttcat ataattttaa cttagctttt aacggtggtt
     4021 gcttcttcat tgcgccaaag ccaacatttg cgaattcatc agttgttccc cagttatgtg
     4081 agatatgcat aacatcgata tattcagcaa tatctaaata acgatcttga ggcaatgtta
     4141 ggtttgaatt catttgtgta tatatacctc gatgatgtgc atactttaat agaggtttaa
     4201 caacatttct aatagacttt ttagaaaaca ttggttcgcc acctgtaatt gacatcgttc
     4261 gcagatttgg aatttcatca agtctacgat aaattaagtc catatctaaa ggctcggggt
     4321 cgacagtttg taaagtataa ccaactgcac agtggctaca acgcatatta caaagatttg
     4381 tagttgtaaa ttcgatgtta cttaaagtta attggccatg ttctttaaca tcgttatatg
     4441 attcccatgg atcattgtaa atgctaacag gttgcttcaa tttgacgcta ttcatcgtct
     4501 taaacttcct tctttctatt tggttcatat cggtatgata ccacttcgta ttatcactcg
     4561 taacaaatta tctcttaatt ttagttaatt gaaaagggga tttttaaaag tcaaaataat
     4621 ttgaatgtca taaatatagc taagattata tcgctttaac gcaatttctg ttaggataat
     4681 aatgaaaaag aaaacaaaat aaaaggagtt aactttttat gaatgaacag caaacaatcg
     4741 aacagataaa agcacgttta aataagttta ttgaggatat cgatcatgta aatcctgatg
     4801 aagtacgtgt tgaagatata gatgaatgga ttggattgtt agatcagctt gaagaaaagg
     4861 ttaaattagt atctaagtaa atgccacata agatgtgtta gagttagata tcgcgatagt
     4921 cgcttaatat tttttatatc ttgtatgttt aatcactgat ttacttgtct atagtaatta
     4981 tatcaatgat atatagaaat gtgattttca atgactaaaa aagtagcaat tattctagca
     5041 aacgaatttg aagatataga atattcaagt cctaaagagg cattagagaa tgctggattt
     5101 aatactgtag tgattgggga tactgcaaat agtgaagttg ttggtaaaca cggtgaaaaa
     5161 gttactgtcg atgtaggcat tgcagaagct aaaccagaag attatgatgc attattaatt
     5221 cctggaggat tttcaccaga tcatttacgt ggagatacag aaggtcgata tggcacattt
     5281 gctaaatact ttactaaaaa tgatgtacca acatttgcca tttgtcatgg gccacaaata
     5341 ctaatagata cagatgattt aaaaggtcgt acgttaacag cagtattgaa cgtgcgcaaa
     5401 gatttatcaa atgcaggcgc acatgtagtt gatgagtcag tagttgtaga caacaatatt
     5461 gtaacaagtc gagtaccaga cgatttagat gattttaatc gagaaatcgt taaacaatta
     5521 caatagaagt attgtggtta tcgattgtta aaatttatat attttcgttt gaaatgaaat
     5581 atggatttta gttttaataa atgaataaca aataaaaggt cgtctctatt ggcatttaat
     5641 agggatggtc ttttatttta caaaatataa caatatttgg catgattaat atttaaaatt
     5701 gtaatgattt atctataatc ttttcaaaaa acaaaaaagt aagtatgatg gcgtatacga
     5761 ttgtaagccc ttggactgat tttcccaaaa agtgtagcct atttgtgata gtactggtaa
     5821 actcaaggta tatactaagt gagttttaaa agaaggagca aacgcatgaa aagaagcgat
     5881 aggtactcaa actcaaatga acattttgag catatgaaac acgaacctca ctataatacg
     5941 tattatcaac cagttggcaa accgccgaaa aagaaaaaaa gtaaacgaat actattaaaa
     6001 atattattaa ccattctaat cattatcgca ttgtttattg gtatcatgta ttttttatct
     6061 acacgcgata atgtggatga actaagaaaa attgaaaata aaagtagttt tgtgtcagct
     6121 gataacatgc cagagtatgt taaaggtgcc tttatttcaa tggaagatga acgattctac
     6181 aatcatcatg gattcgattt gaaaggtaca actagagctt tattttcaac gattagcgac
     6241 agagatgtgc aaggtggtag taccattaca caacaagttg tcaaaaatta tttttatgat
     6301 aatgatcgtt catttactag aaaagtaaaa gaattatttg tagctcatcg agttgaaaaa
     6361 caatataata agaacgaaat tttaagcttt tatttaaata atatttactt tggggataat
     6421 caatatacgc ttgagggcgc agcaaaccat tactttggaa caaccgtgaa taaaaatagt
     6481 acaacaatgt ctcacataac agttttacaa agcgctattt tagctagtaa agtcaatgca
     6541 cctagcgtat ataatatcaa taatatgtca gagaatttta cgcaacgtgt aagcacgaac
     6601 ttagaaaaaa tgaagcaaca aaattatatc aatgaaacac aatatcaaca ggctatgtca
     6661 caattaaatc gttaaatact tgcattcatg tgtaagatgc ttggaactat aatgtagaag
     6721 gtttaattga aatgtcataa tgataaagtt gaattgtttc tgcttaataa attttacaga
     6781 tttgcttgta atcacacttt cattttaaaa gatgatagtg tgattttttg ttagcatcga
     6841 aaaatattta aagttaaaca tttgctatag gaagtggctt attgaataat aaaagagtat
     6901 tgtataaaac ttgagaaagt aacggggtga tccagtgccg aagattacta aaatagaagt
     6961 tcaaaagaaa aacaaagaac gttttaatct ttttttagac gaacaatttg aaatgggtat
     7021 agatatcgat acattagtca aatttaattt aaaaaaaggg caacaacttg aagctgctga
     7081 catggcagag attcaaaagt atgatcatta tcgcataggt ttaaacaaag caatccaata
     7141 tttatcatat aaaaagagaa ctgaaaaaga agttattcaa tatttacaaa aagaagagat
     7201 atcagagcaa gcgatttctg aagtgattga atattgttat cgcgaaaagt taatcgacca
     7261 tcaagattat gcggaaagtt taaaaaatac aatgattcgc acgacagata aaggacctaa
     7321 aatttatcaa caaaaacttt atcaacttgg tatagaacca aatatcattg aaatgtttac
     7381 agaactttat agagaacaac aggaacttga tgatattatc caaattgctg aaaaaatatc
     7441 taaaacaaaa aaaggaccac aaaataaggt taaagaaaaa gtaatgcaat ctttaattca
     7501 aaaagggttt gaaatggaaa ccattcatgc tgtattgaat gaaatggatt ttacacaaga
     7561 tgaagcggtt ttagacgatt tattacaacg agatttagaa aaaatttata ataaaaatcg
     7621 aaagaagtac acgcaacaga aattaatttc aaaaactatc gaaggcctta tgagaaaagg
     7681 atataaatat gataaaatta aagctaaatt agaagaaagt ggtattgccg atggaacaga
     7741 agaaattgag tgagatgtct gagccagaat taagacacga aatacaactt tataaagaga
     7801 aaatgagaaa agctgagatg aatggtattt taaatgaata tgacgtttat caaagtaaag
     7861 taattgttgc cgaaagttac ttagtagaca gaaaaaagat agaaattggt aaaatttata
     7921 aactgactga tggcagtaat caatatttta aagtagaaag attaaaaggt atttttgcgt
     7981 gggggtttag atttaatagc gatgaacccg aagaaggctt acctatagcg ttattacaat
     8041 tatagaagga tgaaaataga tgggaagttc aattgtttta aagttactaa aagtaacaca
     8101 ctactatagg aataaacaga ataagaaatg gtatttacct tttggatatg atgctgaaga
     8161 tatcgattta aacaatatta gtttacatat ttatcaagga gaagcattag gtataattgg
     8221 tgaacctgaa tcttccaaag cgttggtagg tcaattgttg gcaggtgcaa ttaaacctga
     8281 taaaggtaaa gtagtttgta ctgaagattt gttctacgga tatattgaag atcaatcgtt
     8341 aattcatcaa actgttgaag cttacacagc gcagttagtt caactatttc catatgaaat
     8401 taatgatcat aaagctgaac agattattca atatgcacat ttaggcgatt ataaaacgaa
     8461 gccggttaac catatttcga aagcggcata cgctcaatta ctattaagta ttgcacgctc
     8521 atcaaaatca aatattatta ttttaaatca tgttattgac tatttaacac cacaatttat
     8581 ggaacgtgcg attgaattaa caaatgatta tattgaaaat aatttaacga ttgtgtcaat
     8641 tggtgatgat attgataaaa tttcacaagt gagtaactat atagcttggt tttcacatgg
     8701 tcaattaaga atggaagggt cacttaaaca agttattcca tcttttaaag aacatgaacg
     8761 tgatcgatta agtttaaact caaaagaaga aattgaaaac tttgatttag attggaaaaa
     8821 gaatcgtaca agaataccag agatgaccta taatttcaaa cgcgttgagc gctataatca
     8881 tgcaaaaccg cctaagtttt tagtgcgttt ttggacttta gcctcaggta ctattttagg
     8941 cttagcattg atgatgttgc tcattttcaa taatatagga attatttcga taacagattt
     9001 tacgaatcgt gctacgatgc aaaatgaaaa taaagatcca tatggcgaaa agttagctta
     9061 tggaattgct tttaatggca gtgtggatat gcaaggggat aaacaagtca caattccaaa
     9121 atatagtgta gttacaatta ctggcgaaaa tagtaaaaat tatcgtgtta ccgccgataa
     9181 taagacttac tatgttagta aagataaatt agaatatttt aacccggcag gtttatatca
     9241 aacgcatagt tttaaaaaat tagcgccata tatgaaatca aattatagta attactatgc
     9301 atactttaac agtcaattac ataaaaagca tagttcagtt ataaaaactt tagttcctga
     9361 tgatgataac cgtttcgttg catccgttac acaacaaccg atacaattac ttttcaatga
     9421 taataatcag ttatacggtt ttgtttatcc aattgtagat aaaaaagaat taaaagataa
     9481 gtttaatatt aacaataaca tttggattac taaagttggg aatggatatt gtattgccaa
     9541 tttgaaagaa gacaaatgga tttatattga attgtaggtg taaagatgct agataatatt
     9601 attttatatt ttaaaaactt accgcatact aaaaggtatg taacagaacg tttaaaacaa
     9661 agttggaaaa gttttctaat cgtattagct gcatgtttaa tacttattat tgcaagtgaa
     9721 acgctatttt cctttagtca tttgaccgat gttaaagagg tgcgatggtt atttagaatt
     9781 attgcattga ttgtttttgc ggtattgatg ttcacaattt atatctctta tcatcattat
     9841 atgaatgact ttttagtgac taagttattt aatatttccg cagcgacgcc agtagttatt
     9901 atgtctattt taagttttat tatgctagtc attttgacga tgattagtgc attggttaaa
     9961 ccagtaaact tcgaaacatc atatatagcg ctattctatt ttattgtgat ggcaactatt
    10021 tttgtaggat taatatccgt tacttttggt cttattcgac tgttaactga aaaaataaat
    10081 atcatatttt atggggtttg tgtattgtgt atcttattgt taccaattat tttcatacca
    10141 aatccaaatc atgtatttat caatcacatc ctaatgctga atccaatgta ttacattgtt
    10201 aatggtatag cacaatctat catatttggt atatcaagta tggaaaacat tccatatcat
    10261 ttttacttta ttttattctt atgtttaata gctgcagtaa atttcgtatt agcaaggtat
    10321 acgacacacg caatttataa taaaacatct aaagtgacac aaactgataa tcaacaagat
    10381 gtttcaaatg atagtacaga cgaggcggat acttcttcat gatgtttagc tgacctgttg
    10441 tgtaaattaa tctgtgagaa tcagaaacaa ttagaatatc atctagatat tgttttccta
    10501 tactgatttt gatatttcga gtcattaaag gtagtttgtt agcaacaata taaattttag
    10561 attttgtaaa agcattcata gttttacatt aaaaaaccgg acagagtaaa aatcgttttg
    10621 attttcattc tgcccggttg ttcatttttt atagattaac tttagtgaat aataacttga
    10681 aattatttag catacagttt acgttgtaac ttatcttctg taaataccca gccaatataa
    10741 gagtggtcta tgacattatc gtttgttaca tgtgcaattg ccacaaatga ataatgatca
    10801 ttttttaaat aacgcaaatc aatcaaacga atttcagtag aaccatctgc taacgttgtt
    10861 gtttgccaac gatagattga tgaaaagttt aaaaaagtac gtatatcttt attacctttg
    10921 actttccata aaatggagtc tgttgataat gtttgacgtt ccactttatc actaaacacc
    10981 acattcctac catatgcttt tccaacatag tcatgtgcat cagtttgtat cgcgacacgc
    11041 cattccataa attttattgt tggcgcaaca aatactttaa cagggtgatg ctcttgttga
    11101 attgctttta aagcttgttg cttaattacg gctctcattt taaaacgaat catgtaataa
    11161 atgattagta aagcgattat aggaaagaag actgcaaatg gatgcaagcc tataacccat
    11221 aatacaatac caatacaaag aactgtgaaa ataatagggt caaatgtgtt aatcacactt
    11281 aattgaatcc atttgtttgt gataggtcta agcgcttgtg taccataaga attgaatata
    11341 tctacaaaga catgtaaaaa tactgcgagc tgagcccaca tccatacatg aaatgggttg
    11401 gttccactaa agaacgtgaa tattaaaaat gtaattaaaa ttggccatag tattgtgaaa
    11461 gggatggaat gcgtgatacc tctatgatgc gaaatatatg ttgcattgtc ctttaattta
    11521 agaacagtat ccccatcagg aattaatgaa ccaacgataa gggttgtagc tgttgcacca
    11581 aacgtagaag ccattgctgg atcttgagtt gcaagtgctg taaggcccac cccaattgcg
    11641 atatgtgttg cagtgtccat atcagttcac tcatttcata atattatata tactattata
    11701 atccaatttc gcaaataact aaattaactt acatactaac catgatttta atgtacaata
    11761 aaaacattat gagtaaagac aattaatttt ggtgaggaaa gggttttata aatgtatcag
    11821 cagtctagtt ttaaagaaaa tttgatacac tggtttgatg aaaatcaaag agaaatgcca
    11881 tggcgacaaa cgaccaatcc gtactatatt tggttaagtg aggtcatgtt acaacagaca
    11941 caagttaaaa cagttattga ctattatcat cgttttgttg aacgatttcc aactgtggaa
    12001 gttttgagtc aagcttcgga agatgaagtg ttaaagtatt gggaaggctt aggatattat
    12061 agtagagctc gaaattttca tacggcgatt aaagaagttt atgataaata tgaaggctta
    12121 gttcccaaag atcctgatca atttaaagcg ttaaaaggtg ttggcccata cacacaagct
    12181 gcagtaatga gcattgcata taatgtacca ctagcaactg tagacggaaa tgtttttcga
    12241 gtatggtcta gattgaatga tgactatcga gatattaaat tacaatcgac gaggaaatcg
    12301 tacgaacaag aattgttacc gtatgttact acagaagctg gaacgtttaa tcaagctatg
    12361 atggaattag gggcattaat ttgtacacca aaaaatccat tatgcttatt ttgtccagtt
    12421 caagaaaact gtgaggcatt tgataaaggt acatttgaaa agttacctgt taaatctaaa
    12481 aatgtaagta aaaaagtaat tgagcaatcc gtctttttaa ttagaaataa tcaaggtcaa
    12541 tatctgttgc aaaagcgtag cgaaaaatta ttgcatggta tgtggcaatt tcctatgttt
    12601 gaaagtgagc atgctagacg taaaatgact gaaaaaatcg gtcatgacat tcaaccagta
    12661 gaaacaccta tttttgagtt gaagcatcaa tttactcatt taacatggaa gattaaagta
    12721 tacgccgctt caggagcaat taacattgaa acattaccag atgacatgat ttggtttaat
    12781 ttaagtgatc gagaccaata tacattccct gtacctatgt caaagattta tcaatttata
    12841 aatgggtaac attcgatgat tggtaatatt atcggaaaac atagtgacat taattttaaa
    12901 aatgtataaa tgatatcttt aaatctcata ggataacaat gtcaaaaagt taaagtgttg
    12961 atttataaaa gtgatgattt tacatacttt aaatgggaca tgtaaatctc tgttgtagta
    13021 cttagcaata ttaccgagtt taatcattaa gtagtaaaat cttattt
//
LOCUS       DANBWH010000062        12978 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.064, whole genome shotgun sequence.
ACCESSION   DANBWH010000062 DANBWH010000000
VERSION     DANBWH010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12978
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.064"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(186..881)
                     /locus_tag="PPY92_001213"
     CDS             complement(186..881)
                     /locus_tag="PPY92_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDJ2625226.1"
                     /translation="MEGNLLQQLFNYYVTNFGYLWDLFFKHLLMSVYGVLFAALIGIP
                     LGILLARYTKLSGFVITIANIIQTVPVIAMLAILMLVMGLGSETVVLTVFLYALLPII
                     KNTYTGIASVDANIKDAGKGMGMTRNQVLRMIELPLSVSVIIGGIRIALVVAIGVVAV
                     GSFIGAPTLGDIVIRGTNATDGTTFILAGAIPIAIIAIVIDVLLRFLEKRLDPTTRHR
                     KNQSNHRPQSINM"
     gene            complement(881..1822)
                     /locus_tag="PPY92_001214"
     CDS             complement(881..1822)
                     /locus_tag="PPY92_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HDJ2625227.1"
                     /translation="MKKIKYILVVFVLSLTVLSGCSLPGLGSKSTKNDVKITALSTSE
                     SQIISHMLRLLIEHDTHGKIKPTLVNNLGSSTIQHNALINGDANISGVRYNGTDLTGA
                     LKEAPIKDPKKAMIATQQGFKKKFDQTFFDSYGFANTYAFMVTKETAKKYHLETVSDL
                     AKHSKDLRLGMDSSWMNRKGDGYEGFKKEYGFDFGTVRPMQIGLVYDALNTEKLDVAL
                     GYSTDGRIAAYDLKVLKDDKQFFPPYAASAVATNELLRQHPELKTTINKLTGKISTSE
                     MQRLNYEADGKGKEPAVVAEEFLKKHHYFDKQKGGHK"
     gene            complement(1839..2474)
                     /locus_tag="PPY92_001215"
     CDS             complement(1839..2474)
                     /locus_tag="PPY92_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDJ2625228.1"
                     /translation="MIEFLHEHGGQLMSKTLEHFYISIVALLLAIIVAVPIGILLSKT
                     KRTANIVLTVAGVLQTIPTLAVLAIMIPIFGVGKTPAIVALFIYVLLPILNNTVLGVQ
                     NIDSNIKEAGKSMGMTQFQLMKDVELPLALPLIIGGIRLSSVYVISWATLASYVGAGG
                     LGDFIFNGLNLYDPLMIVTATVLVTALALGVDALLALVEKWVVPKGLKVSG"
     gene            complement(2471..3697)
                     /locus_tag="PPY92_001216"
     CDS             complement(2471..3697)
                     /locus_tag="PPY92_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDJ2625229.1"
                     /translation="MLSIKHLTKIYSGNKKAVDDISLDIQSGEFIAFIGTSGSGKTTA
                     LRMINRMIEATEGQIEIDGKDVRSMNPVELRRNIGYVIQQIGLMPHMTIKENIVLVPK
                     LLKWTKEEKDKRAKELIKLVDLPESFLERYPAELSGGQQQRIGVVRALAAEQDIILMD
                     EPFGALDPITRDTLQDLVKTLQRKLGKTFIFVTHDMDEAIKLADKICIMSEGKVVQFD
                     TPDNILRHPANDFVRDFIGQNRLIQDRPNDKTVEGVMIKPITIQADATLNDAVHIMKQ
                     KRVDTIFVVDSNNHLLGFLDIEDINQGIRGHKSLRDTMQQHIYTVQIDSKLQDSVRTI
                     LKRNVRNVPVVDDKQRLVGLITRANVVDIVYDTIWGDSEDAVQTEHVGEDTVSSKMHE
                     QHTTNVKVRDIGDDKS"
     gene            complement(4024..4143)
                     /locus_tag="PPY92_001217"
     CDS             complement(4024..4143)
                     /locus_tag="PPY92_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625230.1"
                     /translation="MGGCPSVTMDACALLQKFDFCNNISHFRHFFAIKQPIER"
     gene            complement(4223..4363)
                     /locus_tag="PPY92_001218"
     CDS             complement(4223..4363)
                     /locus_tag="PPY92_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625231.1"
                     /translation="MSKAYRLYNNIQDDTKQPMAHAVGCFFRCVCHGQHFDVGIPLQA
                     WE"
     gene            complement(4367..4966)
                     /locus_tag="PPY92_001219"
     CDS             complement(4367..4966)
                     /locus_tag="PPY92_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family protein"
                     /protein_id="HDJ2625232.1"
                     /translation="MSEKQSNEQVEAFLNKESQWQEEYKYLRALIFNETELEEAYKWM
                     HPCYTLNNKNVVLIHGFKNYVALLFHKGAILEDKYHTLIQQTEKVQAARQLRFENLTE
                     IQARTEEIKYYLAEAIKAEKAGKKVEMKKTEEYVIPKELEAKFEEMPQLESSFYKLTP
                     GRQHQYIYHIGQAKRSETRQKRVEKYINQILEGKGMHDK"
     gene            5146..6783
                     /locus_tag="PPY92_001220"
     CDS             5146..6783
                     /locus_tag="PPY92_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDJ2625233.1"
                     /translation="MNKDKQLHNDKINLSQLVLLGLGSLIGSGWLFGAWEASSIAGPV
                     AIISWVLGFLVIGTIAYNYIEIGTMFPQSGGMSNYAQYTHGSLLGFIAAWANWVSLVT
                     IIPIEAVSAVQYMSSWPWHWAKPMRYLMENGSISTYGLLAVYLIIVIFSLLNYWSVKL
                     LTSFTSLISVFKLGVPMLTIIMLMLSGFDTSNYGHSASTFMPYGSAPIFAATTASGII
                     FSFNSFQTIINMGSEIKNPEKNIARGIAISLSISAVLYIILQSTFITSMPQSMLQHSG
                     WNGINFNSPFADLAILLGINWLAILLYIEAFVSPFGTGVSFVAVTGRVLRAMEKNGHI
                     PKFLGKMNEKYHIPRVAIIFNAIISMIMVTLFRDWGTLAAVISTATLVAYLTGPTTVI
                     ALRKMGPTMTRPFRAKILKVMAPLSFVLASLAIYWAMWPTTAEVILIIILGLPIYFFY
                     EYRMNWRNTKKQIGGSLWIIVYLIVLSILSFIGSKEFKGLNMIHYPFDFIVIIVVALI
                     FYYIGTTSSFESVYFRRATRINTKMRESLNNESKSSH"
     gene            7092..8399
                     /locus_tag="PPY92_001221"
     CDS             7092..8399
                     /locus_tag="PPY92_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HDJ2625234.1"
                     /translation="MKINTTGGQIHGITQDGLDIFLGIPYAEPPVHDNRFKHSTLKTQ
                     WSEPIDATEIQPIPPQPDNKLEDFFSSQSTTFTEHEDCLYLNIWKQHNDQTKKPVIIY
                     FYGGSFENGHGTAELYQPAHLVQNNDIIVITCNYRLGALGYLDWSYFNKDFHSNNGLS
                     DQINVIKWVHQFIESFGGDANNITLMGQSAGSMSILTLLKIPDIEPYFHKVVLLSGAL
                     RLDTLKSARNKAQYFQKMMRDYLDTDDVTSLSTDDILMLMAKLKQSRGPSKGLDLIYA
                     PIKTDYIQNDYPTTKPIFACYTKDEGDIYITSEQKKLSPQRFIDIMELNDIPLKYEDV
                     QTAKQQSLAITHCYFKQPMKQFLQHLNIQDSNAQLWLAEFAWHDTSSAHYRSAYHILD
                     MVFWFGNLQILAAHQYPTTAHLKFLSRQMQNDLANFAKSGKMP"
     gene            complement(8506..9693)
                     /locus_tag="PPY92_001222"
     CDS             complement(8506..9693)
                     /locus_tag="PPY92_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDJ2625235.1"
                     /translation="MSIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQL
                     VTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRIIS
                     SAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYT
                     FLFLIIVSIIVGFLMMIYLPKDQEIQRGPVNHEAPSHENHVTSKILRPAEVAKYLIIT
                     FLVLIANSVTFVFINPLILSNGHDMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRW
                     LMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQV
                     MSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVVFTLKNNHYAKTF
                     KSS"
     gene            complement(10089..10865)
                     /gene="fetB"
                     /locus_tag="PPY92_001223"
     CDS             complement(10089..10865)
                     /gene="fetB"
                     /locus_tag="PPY92_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HDJ2625236.1"
                     /translation="MSNTALGLTALLLVIPIIISYKEGLHIIKDLIVATLRAVVQLII
                     LGFLLHYIFKINDKWLLILCVLVIIINASWNTISRASPVMHHVFWISFLAIFIGTALP
                     LAGTVATGAIHFTANEVIPIGGMLANNGLIAINLAYQNLDRAFVQDGTNIESKLSLAA
                     TPKLASKGAIRESIRLAIVPTIDSVKTYGLVSIPGMMTGLIIGGVPPLQAIKFQLLVV
                     FIHTTATIMSALIATYLSYGQFFNARHQLVARNTDVKSES"
     gene            complement(10858..11520)
                     /locus_tag="PPY92_001224"
     CDS             complement(10858..11520)
                     /locus_tag="PPY92_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDJ2625237.1"
                     /translation="MLLEIKDLVYKASDRIILDHISLKVDKGESIAIIGPSGSGKSTF
                     QKQICNLISPTSGELYFKGKPYNDYDPEELRQRISYLMQQSDLFGETIEDNMIFPSLA
                     RNDKFDRKRAKQLIKDVGLGHYQLSSEVENMSGGERQRIAIARQLMYTPDILLLDEST
                     SALDVNNKEKIENIIFKLVEQDVAIMWITHSDDQSMRHFQKRITIVDGKISKVEELNQ
                     HE"
     gene            complement(11768..12844)
                     /locus_tag="PPY92_001225"
     CDS             complement(11768..12844)
                     /locus_tag="PPY92_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDJ2625238.1"
                     /translation="MNQSVKLLKHLTDVNGIAGYEMQVKEAMRNYIEPVSDQIIEDNL
                     GGIFGKKNAENGQYSIMISGHMDEVGFMVTKIDKHGFISFTPVGGWWNQVMLSQKVTI
                     TTDSGKEIRGIIGSKPPHVLTPEERKKPMEIKNMFIDIGVSSKEEAEEAGVEVGNMVT
                     PYSEFEVLANDKYLTAKAFDNRYGCALAVEVLKRLKDENIGINLYSGATVQEEVGLRG
                     AKVAANTIKPDLAIAVDVGIAYDTPGMSGQTSDSKLGGGPVVIMMDATSIAHQGLRKH
                     IKDVAKEHNIEVQWDTTPGGGTDAGSIHVANEGIPTMTIGVTLRYMHSNVSVLNVDDY
                     ENSIRLVTEIVRSLNDESYKNIMW"
BASE COUNT         4178 a         2538 c         1922 g         4340 t
ORIGIN      
        1 agcattttta caataattaa cgctttttat ccaaaaactt tcatgataat aattgctatt
       61 ttagtgtaaa cgcatacaaa cacaacctat aaaaaggcta ctcaaagatt atttgagctc
      121 cttgagtagc ctcatttttt ataaatcatg aaacgaaatc gctaaattat aaacatcttc
      181 tactattaca tattaatact ttgcggccga tgattagatt gatttttacg atgtcgtgtt
      241 gttgggtcta atcgtttttc taaaaatctt aatagtacat caatgacgat tgcaatgata
      301 gcaatcggaa tcgcacctgc taaaataaac gttgtgccat ccgtcgcatt tgtaccacga
      361 atcacaatgt caccaagcgt aggtgctcct ataaatgatc caacggcaac aacacctatc
      421 gcaacaacca aggcaatacg aatgccaccg ataataaccg aaacagataa cggtaattca
      481 atcattcgta gcacttgatt gcgtgtcatt cccatacctt tgccagcatc cttaatattc
      541 gcatcaacac tagctatacc agtataagtg ttttttataa ttggaagtaa cgcatataaa
      601 aacactgtta aaactactgt ttctgaacct aagcccatga ctaacattaa aatagctaac
      661 attgcaatga ctggaactgt ttgaattata tttgcaattg taattacaaa tccagaaagt
      721 tttgtgtatc ttgcaagcaa gattcccaat ggaataccaa ttaaagctgc aaacagcaca
      781 ccatagacag acattaataa gtgtttgaaa aataaatccc atagataacc aaagttcgta
      841 acataataat tgaataattg ctgtaataaa ttaccttcca ttacttatga ccacctttct
      901 gtttatcaaa atagtggtgt tttttcaaaa attcttctgc gacgacagca ggttctttac
      961 ctttaccatc cgcttcataa ttcaagcgtt gcatctctga agtcgaaatc tttcctgtca
     1021 acttattaat cgtcgtttta agttctgggt gttgccgtaa taattcattt gttgcaacag
     1081 cactcgcagc ataaggtggg aaaaattgtt tatcatcttt aagtactttc aaatcatacg
     1141 cggcaattcg accatctgta gaataaccta atgcaacgtc taacttctct gtgtttaatg
     1201 cgtcgtagac tagacctatt tgcattggtc tcactgtacc aaagtcaaaa ccatactctt
     1261 ttttaaatcc ttcatagcca tcgcctttac gattcatcca tgaactatcc atacctaaac
     1321 gtaaatcttt actatgcttt gctaaatctg aaactgtctc taaatgatat tttttagcag
     1381 tttccttcgt taccatgaat gcatacgtat tcgcaaaacc atacgaatca aaaaacgttt
     1441 gatcaaattt ctttttaaat ccttgttgtg tggctatcat tgctttctta ggatctttaa
     1501 ttggtgcttc cttcaaagct cccgttaaat ctgtgccatt atatctaaca cctgatatat
     1561 tagcatcccc attaattaag gcattatgtt gaatcgtact tgaccctaaa ttatttacta
     1621 atgttggctt tatcttacca tgtgtatcat gctctattaa caaccgtaac atatgtgaaa
     1681 taatttgcga ttcgcttgtt gataatgctg taattttgac atcatttttc gtgctcttac
     1741 tacctagtcc gggcaaacta catccagata atacggtaag cgataagaca aacacgacaa
     1801 gtatatattt aattttcttc attatcttag cctcctaatt atccagatac ttttaagcct
     1861 ttgggaacta cccatttttc aactaaagct aataaggcat caacacctaa tgctagtgca
     1921 gtaacgagta ccgttgcagt tacaatcatc agtggatcat ataaatttaa accattgaaa
     1981 atgaaatcac ctaatccacc cgcacctaca taacttgcaa gtgtagccca actaattaca
     2041 tacacagatg acaaacgaat gccaccaatg ataagcggca atgctaacgg caattcaaca
     2101 tccttcatca attgaaattg tgtcattccc atactttttc cagcttcttt aatgttgcta
     2161 tcaatatttt gaacaccgag taccgtgtta tttaaaatag gtaataatac ataaataaat
     2221 agcgctacaa ttgcaggcgt tttaccaaca ccaaaaatcg gtatcataat agcaagtaca
     2281 gctagtgttg gaatagtttg taagacacct gccacagtta atacaatatt ggcagttcgc
     2341 tttgtttttg ataataaaat gcctataggt actgcaacaa tgatggcaag taataatgcc
     2401 actatagaaa tataaaaatg ttccagtgtt ttcgacatca actgtccacc atgttcatgt
     2461 aggaactcaa tcatgattta tcatctccta tgtcacgtac tttgacatta gtagtgtgtt
     2521 gctcatgcat ttttgaggac acagtgtctt cccccacatg ttctgtttgc actgcatcct
     2581 cactatcgcc ccaaatcgtg tcatatacaa tgtcaacaac attggcacgc gtaatcagtc
     2641 ctactaaacg ttgcttatca tctacgacag gtacattcct aacgtttctt tttaaaatcg
     2701 tacgtacaga atcttgtaat ttagaatcaa tttgaacggt ataaatatgt tgttgcatgg
     2761 tgtctcgtaa acttttgtgt ccacgtatac cctgatttat atcttcaatg tctaagaaac
     2821 ctagtaaatg gttattacta tctactacaa aaatagtatc aacacgcttt tgtttcataa
     2881 tatgaacagc atcattaagc gttgcatctg cctgtatcgt gattggttta atcattacac
     2941 cttctacagt cttgtcattg ggacggtctt gaatcagtct attttgtcct ataaaatcac
     3001 gtacaaaatc atttgcggga tgtcttaaaa tattgtctgg tgtatcaaat tgcaccacct
     3061 taccttctga cataatacaa attttgtctg ctaatttaat cgcttcatcc atatcatgtg
     3121 ttacaaagat aaacgtcttg cctaattttc gttgtaacgt tttaactaaa tcttgtaacg
     3181 tatctctcgt aataggatcc aatgcaccaa aaggttcatc cattaaaata atatcttgtt
     3241 cggccgcaag tgctcttacg acaccgatac gttgttgttg cccacctgat agttctgctg
     3301 gataacgctc taaaaatgac tccggtaaat ccacaagttt aattaattcc tttgcacgtt
     3361 tatccttttc ttctttagtc catttcaaca atttgggtac caatacaata ttctctttaa
     3421 tcgtcatatg aggcattaac ccaatttgtt gaataacata accaatattt ctacgtaatt
     3481 cgacaggatt catactccga acatctttac catcaatttc aatttgtcct tctgtcgctt
     3541 caatcatacg gtttatcatt cttaaagcag ttgttttacc acttccactg gttccaataa
     3601 atgcgataaa ttccccagat tgaatatcta aagagatgtc atctactgcc tttttattac
     3661 cagaataaat tttcgttaaa tgcttaatac ttaacataat catttctcct tcctcataaa
     3721 aaatatgacg aatgatacgc ataaaataaa aagaccatta tcgcaattgt taaacaaata
     3781 gagtcataag ttaaacaagt acacatcatg gtcttgatta tgtagaattt tatatttttc
     3841 atctgtaatg tgaatactta acagtaaacg gcgctatgta tgttgaattt ctaccgatta
     3901 ctttgtttgc acattcgtat attcgacatt taaaactaac atactctata tgatgttatt
     3961 aaacattatt attttatctt tggcaactaa atagataact catatttttt aaaagcgaat
     4021 ttattatcgc tcaattggtt gttttatagc aaaaaagtgt cgaaaatgtg aaatattatt
     4081 acaaaagtcg aatttttgta ataatgcaca cgcatccatt gttacagacg gacaaccgcc
     4141 catccgccac tcacaaagtc atccttatat tttaatatct tcatcattca ttgtagacac
     4201 ccttgccttt tgctaacatt ttctactccc aagcctgtaa cggaattcca acgtcaaagt
     4261 gttgcccatg acagacacac ctaaaaaaac aaccaacagc atgagccatt ggttgtttcg
     4321 tgtcatcttg tatgttgttg tataaccggt atgctttact cattaattac ttatcatgca
     4381 tccctttacc ttctagtatt tggttaatat acttttcaac acgcttttgt cttgtttcac
     4441 tgcgtttagc ttgtccaata tgatatatat attgatgttg tctgcctggc gttaatttat
     4501 aaaatgaaga ctctaactgt ggcatttctt cgaacttagc ttctaactct ttaggaataa
     4561 catattcctc tgttttcttc atttcaactt ttttaccagc tttttcagct ttaattgctt
     4621 cggctagata atatttaatt tcttcggtac gtgcttgaat ctctgttaaa ttttcaaatc
     4681 gtaactgacg agctgcttgc accttttcag tctgttgaat gagtgtatga tatttatcct
     4741 ccaaaatggc acctttatga aataatagtg caacataatt tttgaagcca tggataagta
     4801 ctacattttt attattcaac gtgtaacaag gatgcatcca tttatacgct tcctctaatt
     4861 ctgtttcatt aaagattaac gctctcaaat atttatattc ttcttgccat tgactctctt
     4921 tatttaaaaa tgcttcaact tgttcatttg attgtttctc actcatagtc atcacttcca
     4981 ttttgttttt caatacacat tgtattatat atcctaattt tataaaaaac taattttaca
     5041 aatgcttttg cgttcttaca aaaaatgcat ttgactatta ttataataag cgtataattg
     5101 tcgcatatta ttttttgtat ttttggcaat aacgaaggag tatttatgaa taaagacaag
     5161 caattgcaca acgacaaaat caatctatcc caattagtct tattagggtt aggctcttta
     5221 ataggatctg gttggctatt tggtgcgtgg gaagcatcat caatagctgg accagtagca
     5281 atcatatcat gggttcttgg attcctagtc attggaacca ttgcctataa ctacattgaa
     5341 atcggcacaa tgtttcctca atcaggtggc atgagtaact atgcccagta tacacatggc
     5401 tcattattag gctttattgc tgcttgggcg aattgggtgt ctttggtgac aataatacct
     5461 atcgaagctg tgtcagctgt tcaatatatg agttcttggc cgtggcattg ggcgaaacca
     5521 atgagatatt taatggaaaa tggctctatt agcacatacg gattgctagc tgtatatctc
     5581 atcattgtta ttttttcatt attaaactat tggtccgtaa aacttttaac atcatttacg
     5641 agtttaattt ctgtatttaa attaggcgta cccatgttaa ccatcatcat gttgatgtta
     5701 tcaggattcg acacttcaaa ttacggccat tcggcaagca catttatgcc ttacggaagt
     5761 gcaccgattt ttgctgcaac aacagcatca gggattattt tttcattcaa ttcattccag
     5821 acaattatta atatgggttc agaaattaaa aatcctgaaa aaaatatcgc aagaggcatc
     5881 gctatttcac tgtcaatcag tgcagtgttg tacatcattt tacaaagtac gtttatcact
     5941 tctatgcctc aatcaatgtt acaacatagt ggatggaatg gcatcaactt caattcacca
     6001 tttgcagatt tagctatctt attaggaatt aattggctcg caattttact atatattgaa
     6061 gcttttgtat caccattcgg tactggcgtg tcatttgtcg ccgttacagg tcgagtttta
     6121 cgagcaatgg agaaaaatgg acatatccct aaatttcttg ggaagatgaa tgaaaaatat
     6181 catatcccac gtgtagcaat catctttaat gccatcatta gtatgattat ggttacatta
     6241 tttagagatt ggggtacgct agcagcagtt atttctactg caactttagt agcctattta
     6301 actggcccaa cgacagtgat tgcattaaga aaaatgggac caacaatgac tcgtccattt
     6361 agagcaaaaa ttttaaaagt aatggcacca ttatcatttg tattagcttc attagctata
     6421 tattgggcaa tgtggccaac aacggctgaa gttattttaa tcattatact tggattacca
     6481 atctacttct tctatgaata ccgtatgaat tggcgtaata caaagaaaca aattggtggt
     6541 agcttatgga ttattgtata tttaatcgtg ctatcaatac tgtcatttat aggaagcaaa
     6601 gaatttaaag gcttaaatat gattcactat ccatttgact ttatcgttat tattgttgtg
     6661 gcgcttatat tctattacat cggtacaacg agttcatttg aaagcgtcta tttccgtcgc
     6721 gcaacacgaa tcaatacaaa gatgcgcgag tcactaaata atgaatcaaa gtcatctcat
     6781 taaatatttt aaaatgcaat gccatatatg atttcaacac acacacatta accaaccatt
     6841 gatttcaaca tcttggttgg ttttttattt tgaaaatcgg ttataaataa ctaacataac
     6901 aatgtggcga gagtgatatt gtaatgaaat gtcactgtag aagaacatca tataatgctg
     6961 aaaatggttt gagttaaaac agatatcaag ctagtctgat tcagtcacaa aattgtcttg
     7021 ttatacttgt cacctatcat ctatagaccg tggtatgatt aaattgggga tgataaagga
     7081 ggttaataaa tatgaagatt aatactacag gtggtcaaat tcatggtatt acacaagatg
     7141 gtttagatat cttcttaggc attccttatg cagaaccacc agttcatgat aatcgcttta
     7201 aacattctac gttaaaaaca caatggtcag agccaattga tgcaactgaa atacaaccca
     7261 tcccaccgca accagacaac aaattagaag attttttctc ctcacaatct acaactttta
     7321 ctgaacatga agactgttta tatctaaata tttggaaaca acataatgat cagacgaaga
     7381 aacctgtcat catttatttt tatggtggta gttttgaaaa tggtcatggt acagccgaac
     7441 tctatcaacc ggcacattta gtacaaaata acgacattat cgttattaca tgcaattatc
     7501 gtttaggcgc attaggatat ttagactggt catattttaa taaagatttt cattccaata
     7561 atggactttc agatcaaatc aatgtcataa aatgggtgca tcaatttatt gaatcctttg
     7621 gtggcgacgc taataacatt actttaatgg gtcagtctgc aggcagtatg agcattttga
     7681 ctttacttaa aatacctgac attgagccat acttccataa agtcgttcta ctaagtggcg
     7741 cactacgatt agacaccctt aagagtgcac gcaataaagc acaatatttc caaaaaatga
     7801 tgcgcgatta tttagataca gatgatgtta catcattatc gacagatgat attcttatgc
     7861 tgatggcgaa gctaaaacaa tctcgaggac cttctaaagg gcttgattta atatatgcgc
     7921 ctattaaaac agattatata caaaatgatt atccaacaac gaaaccaatt tttgcatgtt
     7981 atacaaaaga tgaaggcgat atttatatta ctagtgaaca gaaaaaatta tcgccgcaac
     8041 gctttatcga cattatggaa ttaaatgata ttcctttaaa atacgaagat gttcagactg
     8101 cgaagcaaca atctttagcg attacacatt gttatttcaa acagccgatg aagcaatttt
     8161 tacaacatct taatatacaa gattcaaacg cacaactatg gcttgctgaa tttgcatggc
     8221 atgatacttc tagcgcacac tatcgcagtg cttaccatat tctagatatg gtattttggt
     8281 tcggaaactt acaaatatta gcagcacatc aatatccaac aactgctcac cttaaatttt
     8341 taagtcgaca aatgcaaaat gatttagcta attttgctaa aagcggtaaa atgccttgac
     8401 cgatgtatca taatgaacgt cgctattatc gcacatatca ataacgttat cgcatctaaa
     8461 aaagcacacc tgaatttgat agcaggcgtg cttttcatat gagaattatg atgatttaaa
     8521 tgttttagca taatgattat ttttcaatgt gaaaacaacg attaatccta atgcgccaat
     8581 gatggcactc gtatatgtga tagcttgaac agaaacatgt gtcatgacca agcctccaat
     8641 aatgccgcca acaccaatac cagcgtttaa actagacatg ttccaactca ttacttggct
     8701 tgtgtcgcct tcaacatgtt gaatcacacc gctttgcact gctggattag tactccattg
     8761 catgatattc caaataaata gtcctgctaa caatagacct gaaccaggta agattaaatt
     8821 cataagtaac atcataacga taaaaataga aacagaaatc attaaccaac gcttacttgt
     8881 aattttatcg gagaatatac cacccaatga tgttccaata acgccagcga ttccatttac
     8941 tagaagtgct aatgaaacga atgacatatc atgaccatta gataaaataa gtggatttat
     9001 aaagacgaat gtcactgagt ttgcaatcaa tactaaaaac gtaataatta aatattttgc
     9061 tacttcagca ggtcttaata ttttcgaagt aacatgattt tcatgagatg gtgcctcatg
     9121 attcacaggg cctcgttgta tttcctgatc cttcggtaaa tagatcatca tcaagaagcc
     9181 aacaataata ctcacaataa ttaagaatag aaatgtatag cgccaaccta ctaaatcgcc
     9241 gataaccgtt ccaattggta caccaaaaac attggcccca ctaaaccctg tatagacaag
     9301 tccaatcatt ttgccacgat ttttaggtgc tgataacatc gctgtaatag ctaatacttt
     9361 tacgataatt agtgctgctg ctgcagatga gataattcta ccaactacta atattgaaaa
     9421 atttggcgct acagcaataa tgccattacc aatgataaat ataagtaatg tccataataa
     9481 tacaggcctt gatgaaaaac ggttcgttaa tttaaccaga ataggtccac atatcgcaaa
     9541 tgttaaagcg tacattgtca ctaattgacc aacgacagct tctgatacat gtaagtcctg
     9601 actcatcaag ttcataattc ctgcaaccat catttcaacc attcctacga taaaaatact
     9661 taaaatgaat gtaaatagtc gcatgattga cattttaaaa ctcctttcaa acaaaatttc
     9721 cgatattcat ttcccttaga ataacgattt attaaaaaat gtgtgcaaca caattttata
     9781 tataaccgtt ctattatata atattttgct ttaattttct gtgcttaaat ggaaaattat
     9841 tgaaattgtt tttcagtatt tgaaaaatta acacaagtat gtcatttaat aattatgtat
     9901 aattacttca gtttcaacaa tgtcttcaaa aaagcacgca cgctttatat gtcagaacag
     9961 ataactgact acaaagtatg cgtgccttta tgaatatttt cgctgtgtta atgaatagcg
    10021 atactatatg tgcgcgtcac ttcatttcca attaaaacta atgcctaaat ctgatgcagt
    10081 aaaatctatc atgattcact cttaacatcc gtatttcgtg ctactaattg atgtcttgca
    10141 ttgaaaaatt gaccatagct taaatatgtc gcaatcaaag cagacataat ggtcgcagtt
    10201 gtatgaataa acacgactaa caattgaaat ttaatcgctt gtaaaggtgg tacgccacca
    10261 ataattaagc ctgtcatcat accaggaatc gacacaagcc catatgtttt aaccgaatca
    10321 attgttggca ctatagctaa acgaatactt tcacgtattg cacctttaga agccaattta
    10381 ggtgtagctg caagtgataa tttagattca atattagtac catcttgtac gaatgcacga
    10441 tctaaattct ggtaagctaa attaattgca atcaagccat tatttgcaag catgccgccg
    10501 ataggtataa cttcattggc tgtaaaatga attgcccctg tagctacagt acctgcaagt
    10561 ggtaatgctg ttccaatgaa gatagctaga aatgatatcc aaaacacatg atgcatcact
    10621 ggtgatgctc gactaattgt attccatgat gcattaataa taatgaccaa tacacataaa
    10681 ataagcagcc atttatcgtt tattttaaaa atataatgca gcaaaaatcc caaaatgatt
    10741 aattgcacca ctgcgcgtag tgtcgcaaca attaaatctt taataatatg taaaccttct
    10801 ttatatgaaa tgataatcgg aatgactaaa agcaatgccg tcaatccaag tgccgtatta
    10861 ctcatgttga ttcaactcct caaccttaga aattttgcca tcaacaatcg ttatgcgctt
    10921 ttgaaaatgt ctcatacttt gatcatcact atgggtaatc cacatgatag caacatcctg
    10981 ttccactaac ttaaaaatga tattttctat cttttcttta ttattaacgt ctaatgcact
    11041 ggtcgattca tctaataaaa gaatatccgg tgtatacatc agttggcgcg ctatagcaat
    11101 tctttgccgc tcaccacccg acatattttc cacttccgaa cttaattgat aatgtcccaa
    11161 accgacatct ttaattaatt gctttgcacg ttttctatca aatttatcat tacgtgcaag
    11221 tgatgggaat atcatgttat cttcaatcgt ttcaccaaac aagtcacttt gctgcatcaa
    11281 ataactgatt cgttgacgca attcttccgg gtcataatca ttatagggtt tacctttaaa
    11341 ataaagttct ccactagttg gactaatcaa attacatatt tgcttttgaa atgtactttt
    11401 accactacct gatggaccta taatggcaat actctcgcct ttatctactt ttagactgat
    11461 atgatctagt atgattctat cgctcgcttt atacactaaa tctttaattt ctaacaacat
    11521 ttgcaccgct cctcatgtaa tatatcaggt atattattcc cgtttttttg gtttttagtg
    11581 caattacatt taagaaaaaa tcattttctt gtaatatcaa aatttaggca taattgattt
    11641 tcagcattta aattaaaaac aaaaaatcct attaacatgg atactgtcta atgactaaga
    11701 attaaagtta gacaattgtt taacaaccta ccatattaaa aggattcttt attatttatg
    11761 gatttgatta ccacatgata tttttataac tttcatcatt caatgaacgg acaatttcag
    11821 taacaagacg gatagaattt tcataatcat ctacattgag cactgaaaca ttagaatgca
    11881 tgtatcgcag cgtaacaccg attgtcatcg ttggaatacc ttcatttgcg acatgaatac
    11941 ttcccgcatc tgtacctcca cctggtgtcg tatcccattg tacttcgatg ttatgttcct
    12001 tagctacatc tttaatatgc ttacgcaaac cttggtgagc aatacttgta gcatccatca
    12061 taatgacaac tggaccaccg cctagtttac tatcgctcgt ttgacctgac atacctgggg
    12121 tatcataagc aatacctaca tcgacagcta tcgccaaatc tggtttaatt gtattcgctg
    12181 ccactttcgc accacgcaaa ccaacttctt cttgcactgt ggcaccactg tataagttaa
    12241 ttccaatatt ttcatctttt aaacgtttta atacctcaac agctaatgca cagccatagc
    12301 gattatcaaa tgctttcgca gttaaatatt tatcatttgc aagcacttca aattcactat
    12361 atggcgttac catattgcct acttcaacgc cagcttcttc agcttcttcc ttgctactaa
    12421 caccaatatc tataaacata tttttgattt ccattggctt tttacgttct tcaggcgtta
    12481 agacatgtgg cggtttagaa ccgatgatac ctctaatttc tttgcccgaa tctgttgtaa
    12541 tcgttacttt ttgagatagc atgacttgat tccaccatcc accaactggc gtaaatgaaa
    12601 taaaaccatg tttatcaatc tttgttacca taaaaccaac ttcatccata tgaccagaaa
    12661 tcataattga gtattgacca ttctcagcat ttttctttcc aaaaatgcca cccaagttat
    12721 cttcaataat ttgatcactg acaggctcta tatagttacg cattgcttct ttaacttgca
    12781 tttcataacc agcaatgccg tttacatctg ttaaatgttt aagtaatttg actgactgat
    12841 tcatgttatt catcctcctg tatttcaact taaaactatt gtatcactaa ctttcagaaa
    12901 aacgttccct accgctttat cattttcagg cttaaatttt ttaaaatttt gtgagaaatc
    12961 cgcttcaaaa acctcata
//
LOCUS       DANBWH010000063        12955 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.065, whole genome shotgun sequence.
ACCESSION   DANBWH010000063 DANBWH010000000
VERSION     DANBWH010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12955
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.065"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            225..575
                     /locus_tag="PPY92_001226"
     CDS             225..575
                     /locus_tag="PPY92_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002502216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625239.1"
                     /translation="MKTTTQELKQYITRLFQLSNNETWECEALEEAAENILPERFIND
                     SPLAHLTLETYTYYNNELHDLSIYPFLMYANNQLISVGYLNHFDMDFLYLTDTKNTII
                     DERHLLREGGTHHE"
     gene            662..973
                     /locus_tag="PPY92_001227"
     CDS             662..973
                     /locus_tag="PPY92_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625240.1"
                     /translation="MKINRYITRGISEYLSLDLQILLWNMVKERDNQPHTDYLHIFKL
                     QEDENILSITHEQEQPAYKLEYHYTNYVKNQNALPKKVYVIREDGVDAFYYVMLLPEE
                     Y"
     gene            991..1497
                     /locus_tag="PPY92_001228"
     CDS             991..1497
                     /locus_tag="PPY92_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HDJ2625241.1"
                     /translation="MNTIKNTIYTEAIFSKDEKHRYLLKKTWDEKKPACTVITMYPHL
                     DGVLSLDLTTVLILNQLANSERYGAVYLVNLFSNIKTPENLKHIKEPYDKHTDIHLMK
                     AISESDTVILAYGAYAKRPVVVERVEQVMEMLKPHKKKVKKLINPATNEIMHPLNPKA
                     RQKWTLKA"
     gene            1518..1835
                     /locus_tag="PPY92_001229"
     CDS             1518..1835
                     /locus_tag="PPY92_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HDJ2625242.1"
                     /translation="MKEINIVSLQMIKTDTLSYLKNRISNPEDAAEIMRSFIGNSDRE
                     HLILICMNSKNEPTHIQTLSIGSINQTVIHPREIFKTAILSNANSIMLGHNHPSGDVL
                     TIV"
     gene            1954..3039
                     /locus_tag="PPY92_001230"
     CDS             1954..3039
                     /locus_tag="PPY92_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDJ2625243.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSKEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            3036..4928
                     /locus_tag="PPY92_001231"
     CDS             3036..4928
                     /locus_tag="PPY92_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDJ2625244.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNVDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            4935..5312
                     /locus_tag="PPY92_001232"
     CDS             4935..5312
                     /locus_tag="PPY92_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDJ2625245.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            5463..6245
                     /locus_tag="PPY92_001233"
     CDS             5463..6245
                     /locus_tag="PPY92_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HDJ2625246.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(6371..7102)
                     /gene="erm(A)"
                     /locus_tag="PPY92_001234"
     CDS             complement(6371..7102)
                     /gene="erm(A)"
                     /locus_tag="PPY92_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HDJ2625247.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(7185..7244)
                     /locus_tag="PPY92_001235"
     CDS             complement(7185..7244)
                     /locus_tag="PPY92_001235"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HDJ2625248.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            7636..8298
                     /locus_tag="PPY92_001236"
     CDS             7636..8298
                     /locus_tag="PPY92_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDJ2625249.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            8509..8679
                     /locus_tag="PPY92_001237"
                     /pseudo
     CDS             8509..8679
                     /locus_tag="PPY92_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002453405.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8747..9031
                     /locus_tag="PPY92_001238"
     CDS             8747..9031
                     /locus_tag="PPY92_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625250.1"
                     /translation="MTLFIIIGVLVPMVYTMQLNIKNEPVTKRNLLITLALSTLGILV
                     TALAGVIVTKQAFPLLSVAIGSIFTGIVWGLLLSGSYALIRFLSNAFGRK"
     gene            complement(9148..9345)
                     /locus_tag="PPY92_001239"
     CDS             complement(9148..9345)
                     /locus_tag="PPY92_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625251.1"
                     /translation="MSILTIILIALLVILLFRVGLSILRFLIYVGLVLLCIYLGYQGL
                     IWLLDFFQINSGFLPHFQFNN"
     gene            9505..9978
                     /locus_tag="PPY92_001240"
     CDS             9505..9978
                     /locus_tag="PPY92_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4930 family protein"
                     /protein_id="HDJ2625252.1"
                     /translation="MRLIFSMIKNIIAVIAIVIIVYIALKYAPFLKEQDWNPVNHDND
                     QMNQVTQPTNDAQHVYVSGEKYSLKENDLIKNVPLSQIKNVFKMIDKQEFMAVSGMNR
                     MAYNDQYIIGQRGDEFILYKFGDESMRVYNTEFEMQQDLNELGQNLQLKPENAYQ"
     gene            10052..10171
                     /locus_tag="PPY92_001241"
     CDS             10052..10171
                     /locus_tag="PPY92_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625253.1"
                     /translation="MAEQSKQKQANEQQKAQNLFARWRQLQNSNSESSNDTNK"
     gene            10370..11212
                     /gene="mreC"
                     /locus_tag="PPY92_001242"
     CDS             10370..11212
                     /gene="mreC"
                     /locus_tag="PPY92_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDJ2625254.1"
                     /translation="MLKFFKNNKLIVVLCAIIVFIALIGLSIRSQSQSPPEQYIGDSV
                     SFGQRVVSYPVNFVAGTIGDFFKKGDSKESKNKISQLESKNQQLEAENEKLKKELDLK
                     DISKFDPISTTVLARNPDQWMNTIVIDKGSKSGITSNMAVMTSQGFVGRVTKVNKFSS
                     QVDLISTNTRAGKLSVNIQHGSKNIFGLIDRYDEKNSELVISDINNRDNISKGDKVVT
                     SGLADQLPSNLYIGEVTKVQNDQYGLAKEVRVKTGADLTDLSHVYVAKRDPKTIPDDE
                     SRDK"
     gene            11212..11742
                     /gene="mreD"
                     /locus_tag="PPY92_001243"
     CDS             11212..11742
                     /gene="mreD"
                     /locus_tag="PPY92_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDJ2625255.1"
                     /translation="MRTLYYFLIGILLFYIDTAIGLLIPMHIGKFELVFVPHLTFMYI
                     LMMVVYRGFGVSLLLSIFLGVMTDVYFGSIYGVYLFGYILFLALIDRFFKIFYKDHSM
                     LFIIILASTLLLEVYVALIYGMLGFIQFDIIHFVVFRLLPTLIMNFVLLIMLYPLIIK
                     FLKKTNNDIDMKRRQW"
     misc_feature    11769..11965
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            11978..12286
                     /gene="rplU"
                     /locus_tag="PPY92_001244"
     CDS             11978..12286
                     /gene="rplU"
                     /locus_tag="PPY92_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDJ2625256.1"
                     /translation="MFAIIETGGKQIKVEEGQEIFVEKLDVNEGDTFTFDKVLFVGGD
                     SVKVGAPTVEGATVTATVNKQGRGKKITVFTYKRRKNSKRKKGHRQPYTKLTIDKINA
                     "
     gene            12292..12612
                     /locus_tag="PPY92_001245"
     CDS             12292..12612
                     /locus_tag="PPY92_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDJ2625257.1"
                     /translation="MITVDITVNDEGKVTDVIMDGHADHGEYGHDIVCAGASAVLFGS
                     VNAIIGLTSERPDINYDDNGGHFHIRSVDTNNDEAQLILQTMLVSLQTIEEEYNENIR
                     LNYK"
     gene            12624..12908
                     /gene="rpmA"
                     /locus_tag="PPY92_001246"
     CDS             12624..12908
                     /gene="rpmA"
                     /locus_tag="PPY92_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDJ2625258.1"
                     /translation="MLKLNLQFFASKKGVSSTKNGRDSESKRLGAKRADGQFVTGGSI
                     LYRQRGTKIYPGENVGRGGDDTLFAKIDGVVKFERKGRDKKQVSVYAVAE"
BASE COUNT         4698 a         1845 c         2382 g         4030 t
ORIGIN      
        1 cagagcccca ccctaacatc aatagttata aagaatgagt tgattgttcg tcaaaatagg
       61 cggaatttcg ggatttcctt gtcataccaa ggtttgaagc ggtttttcat atggaatttt
      121 ctctcattct caccgggatg cgtaataaat gacaaatcaa atatatatta tcccaatgat
      181 gtacataaac tacatcacta taacactatc taggaggcaa tgatatgaaa acgaccactc
      241 aagaactcaa acaatatata actcgtctat tccaactatc taacaatgaa acatgggaat
      301 gcgaagcgtt agaagaagca gcagaaaata ttctaccgga acgttttatt aatgattccc
      361 cacttgcaca tcttacactt gaaacttata cctactacaa taatgaacta catgacctaa
      421 gtatctatcc atttcttatg tacgctaata accaactcat cagcgttggt tatctgaatc
      481 attttgacat ggacttttta tatctcacag atactaaaaa tacgattatc gatgaacgtc
      541 atttactaag agaaggaggg actcaccatg aataactgga tcaaagtggc acaaatatct
      601 gttacgatta ttagccaagc gattatcatt atgaaagaaa ttcaagacga ggtaaaataa
      661 catgaaaatc aatcgataca tcacaagagg tattagtgaa tacctatctc tagaccttca
      721 aatcttactt tggaatatgg taaaagaaag agataatcaa cctcatacag attacctaca
      781 catttttaaa ctgcaagaag atgagaatat actctcaatc acacatgaac aagaacaacc
      841 tgcatacaaa ttggaatatc actatacaaa ctatgtaaaa aatcaaaatg cattacctaa
      901 gaaagtctac gtcattcgtg aagatggcgt agacgctttt tattatgtga tgcttttacc
      961 agaagaatac taaaggaagt gattatatat atgaatacaa tcaaaaatac gatatacaca
     1021 gaagctatat ttagcaagga tgaaaaacac cgctatttac tcaagaaaac atgggatgaa
     1081 aagaaacccg cttgtacagt gataacgatg taccctcatt tagatggtgt attatcactc
     1141 gatctcacaa ctgttcttat cctcaatcaa ttagcgaatt cagaacgata cggtgctgta
     1201 tatcttgtaa atctattctc taatattaaa acaccagaga accttaaaca tattaaagaa
     1261 ccttatgata aacacacaga catccacttg atgaaagcaa ttagcgaaag tgacacagtg
     1321 attctagctt atggtgctta tgcgaagcgg ccagttgttg tagaacgtgt cgaacaagta
     1381 atggaaatgt tgaagcctca taaaaagaaa gtcaaaaagc tcattaatcc agccacaaat
     1441 gaaatcatgc acccactcaa ccctaaagca cgtcaaaaat ggactttgaa agcataacct
     1501 atatatggag gtaaacgatg aaggaaatca atattgtttc actacaaatg ataaaaacag
     1561 atacattaag ttatctaaaa aatcgtattt caaaccctga ggatgcggca gaaatcatgc
     1621 gttcattcat tggaaacagt gaccgagagc atctcattct catatgtatg aacagtaaaa
     1681 atgaacctac acatattcaa acactatcga ttggatctat taaccaaacg gtgattcacc
     1741 ctagagaaat attcaaaaca gcgatactca gtaacgcaaa tagtataatg ctcggtcata
     1801 atcatccaag tggagatgta ttaaccatag tataataaat aatagtagtt aaagtagttt
     1861 aagaggagat attaaaggga tattttactt taaataaaaa ttaacaaggt ttataatttg
     1921 tagtagttaa agtatttaaa gaggtgggaa catgtgaagg ttcaaaggat agaagtggag
     1981 aataagccgt atccattgta tttattacta gataaagaat accagctaat agaaccagta
     2041 atgaaattta ttaaatactt agataatact ggtaagtctc ctaataccat taaggcatac
     2101 tgctatcatt taaagttgct gtacgagttc atggaacaga gaggtgttat tcttaatgat
     2161 attaactttg agttgttagc agacttcgta ggttggttga gatatccttc agcatcaaat
     2221 gtaattgatc ttcagtcaaa aaaagccata agagaagaaa cgacagtgaa tacaatttta
     2281 aatgtagtta tgagttttct tgattattta agtagattag gagaatttaa atcaattgat
     2341 gtatttaaac aagcaaaggg aagaaatttc aaaggatttt tacatcatgt taataagggt
     2401 agataccaaa agaatgtctt aaagttaagg gttaaaaaga aacagataag aacattgaga
     2461 tcaaaggaag ttaagcaaat tattgatgct tgtcatacga aaagagataa attaatttta
     2521 atgcttatgt atgagggtgg tttaagaatc ggtgaagtgt tatcgcttag gcttgaagat
     2581 attgtcactt gggacaatca aatccattta acacctagag atgttaatgt taatgaagct
     2641 tatattaaat taaggaagga aagaacaata catgtgagta aagaacttat gtcactttat
     2701 acagattact tgatatatga gtatagtgag gaattggagc atgattatgt ttttatttcc
     2761 ttaaaagaag gctattttgg gaaaccacta aagtaccaaa gtgttcttga tctagttaga
     2821 agaatagtta aaaggactgg aatagaattt acatcacata tgcttcgcca cactcacgca
     2881 acgcagctaa ttagggaagg atgggatgtt gcgttcgttc aaaagagatt aggtcacgca
     2941 catgttcaga caacgttaaa tacctatgtt catctttcag atcaggatat gaaaaatgag
     3001 tttaataaat acctcgagag aaaggagcat aagaaatgaa tgcttctagt aaaaggaaaa
     3061 ttattagtca gagtgagatt agcaaaaaaa tagctgtaat gaatgaagaa atgcaggggt
     3121 tttgggctaa taatagttgg gatataagaa aatgtccaca tccttctgcc atagaattaa
     3181 gtaagaatcc tgctttaagg aatcgttggg ttcgttttga acgtgttaaa aatctgtggt
     3241 taagaacaga attgaaatat ttttattttt accatttaaa caatggaata tggaatgcaa
     3301 aaactgtctg gattagaaaa ggaacagtaa ttaataaaat gttagatttc ttagatttaa
     3361 agtatcctag cattacttca attactgaag ttcctattga aaaagcaatg acggagtata
     3421 gaacttattt gacaaaacgg ggtgttagaa ttaccactac taattataag attactgcta
     3481 atcaagaaaa aacacctgta aaagctaatt cctactatgt tactaatcta aaacaattta
     3541 tggagtttta tgagaacttt tattttgatg gagaggagtg ggataaagac gtttgggata
     3601 gacgtaactt acctttgcca gatgataagg ttaacccaac acaatatgaa tatacaatta
     3661 actttaaagg gtttcggaat acatatttta aacaacttgt aaaaagatat tgtaagttga
     3721 gattgaacgt ggatagcttt tcctatgtaa gtgatattgc ccaaagactt aaagagttct
     3781 ttaattttct ggacatgaaa tttaaacaag ttcagagagt acaccaatta acgagagtgg
     3841 aaattgaagc atatttaagt gaactaaaca tgatgggaat aaaacctagt acaataactg
     3901 ggaggatctc tatattggaa ggactattta gtacccttct taggctagaa tgggatgatg
     3961 ttccttccaa aatattaatt tattctgagg actatccgaa aataccaaga gcaaaaccac
     4021 gctttataga tgaattcgtc ctagagcaat tgaacagtca tcttgataaa ttacccgaat
     4081 atatagctac gatgactatg attgttcaag aatgtggaat gaggataagt gaattgtgca
     4141 ccttgaaaaa aggctgtcta ttagaggaca aagatggaga tttcttttta aagtattatc
     4201 aatggaaaat gaaaaaggag catatagttc caatatctaa agaggtagct ttacttatta
     4261 aagttcggga agataaagtt tcagaggaat ttccagatag tgaatacctc tttccaagaa
     4321 aagatggatc gccattaaaa caagaaacat ttagaggtga gttaaataaa ttagcttatg
     4381 agcaaaatat agtggataaa tcaggtgaga tttatagatt ccatgcccat gcctttcgcc
     4441 atacagtagg aacaagaatg attaacaacg ggatgcccca gcatattgtg cagaaatttt
     4501 tggggcatga aagcccagaa atgacaagca gatacgctca tatctttgat gaaactctaa
     4561 aaaatgaatt tactaaattt caggaaaaac tggttaccaa taatggagat gtgcttgatc
     4621 tagatgaaga taatgaagtc gatgatgtag agcttcaatg gttcaagaaa aatataaatg
     4681 cacaagtgct tccaaatggt tattgtagat tgccagtagt agcaggtggt tgtccacatg
     4741 cgaatgcatg cttagattgc actcacttct gtaccagtaa gcaattctta ccacagcacg
     4801 aagaacagtt agagcgtaca gaagagttat tagccatagc taaggataaa caatggcaaa
     4861 gacaagtaga gactaatagc cgtgttaaag agcgtttaga acaaatcatt ggaagtttga
     4921 cggggtaatt atcaatggat aaacaagtta gaaatacaac agaaattgta cgtttggcga
     4981 agcagaaatc aaaaaagaca agggaaaaag tagacaaagc gatttctaaa ttttcgattg
     5041 aaggtaaagt tattaatttt aattcaatag caaaggaagc taatgtttct aaatcatggc
     5101 tttataagga acacgatatt aggcaaagaa tcgaatccct tcgtgagcgt caaataacag
     5161 caaatgtagt ctcaaaaccc aagaaaagtt ctcgttcgga ggaaatcctt attaaaacct
     5221 taaaaagaag agtaatggaa ttagaaaaag aaaataaaaa attacagaac caaattcaaa
     5281 aattatatgg agatctgtat aataaagaat aattattaat ctgtagacaa attgtgaaag
     5341 gatgtactta aacgctaacg gtcagcttta ttgaacagta atttaagtat atgtccaatc
     5401 tagggtaagt aaattgagta tcaatataaa ctttatatga acataatcaa cgaggtgaaa
     5461 tcatgagcaa tttgattaac ggaaaaatac caaatcaagc gattcaaaca ttaaaaatcg
     5521 taaaagattt atttggaagt tcaatagttg gagtatatct atttggttca gcagtaaatg
     5581 gtggtttacg cattaacagc gatgtagatg ttctagtcgt cgtgaatcat agtttacctc
     5641 aattaactcg aaaaaaacta acagaaagac taatgactat atcaggaaag attggaaata
     5701 cggattctgt tagaccactt gaagttacgg ttataaatag gagtgaagtt gtcccttggc
     5761 aatatcctcc aaaaagagaa tttatatacg gtgagtggct caggggtgaa tttgagaatg
     5821 gacaaattca ggaaccaagc tatgatcctg atttggctat tgttttagca caagcaagaa
     5881 agaatagtat ttctctattt ggtcctgatt cttcaagtat acttgtctcc gtacctttga
     5941 cagatattcg aagagcaatt aaggattctt tgccagaact aattgagggg ataaaaggtg
     6001 atgagcgtaa tgtaatttta accctagctc gaatgtggca aacagtgact actggtgaaa
     6061 ttacctcgaa agatgtcgct gcagaatggg ctatacctct tttacctaaa gagcatgtaa
     6121 ctttactgga tatagctaga aaaggctatc ggggagagtg tgatgataag tgggaaggac
     6181 tatattcaaa ggtgaaagca ctcgttaagt atatgaaaaa ttctatagaa acttctctca
     6241 attaggctaa ttttattgca ataacaggtg cttactttta aaactactga tttattgata
     6301 aatattgaac aatttttggg aagaataaag cgtcctcttg tgaaattaga gaacgcttta
     6361 ttactttaat ttagtgaaac aatttgtaac tattgaaaat agaaagaaat tgttccttcg
     6421 atagtttatt aatattagtg acatttgcat gcttcaaagc ctgtcggaat tggtttttag
     6481 tgaaaagaac acgatattca cggtttaccc acttataaac aaaagatcga tactttttgt
     6541 agtccttctt tgaaatcaat ggttgatgtc gttcaagaac aatcaataca gagtctacac
     6601 ttggcttagg atgaaaatat agtggtggta cttttttgag catttttata tccatctcca
     6661 ccattaatag taaacccaaa gctcgttgca gattttgcaa tcttttcgca aatcccttct
     6721 caacgataag atagctatat ttagcctgac tttcaaaggt aattcttttg acaatatccg
     6781 tactgatgtt ataaggaata ttaccatata tcttatagtt tatatgtttt gggaaggaaa
     6841 attttagaat atccgtttga atcactttta tattctcaga ggggtttacc gcttctttag
     6901 tcacttgaca taagcctcca tcaatttcta tagcagtaac tgatcgactc attttgacta
     6961 gctctttggt aaaatgtcct tttcctgatc cgatttctat tacgttgtct tgtttactga
     7021 tattcgtgtg attcaatatt tcttttacat gctttttaga agtaataaaa ttttgcgtgt
     7081 ctttagggtt tttctggttc attataacct tctccttact ggttcattat aaccttctcc
     7141 ttactggtta taatgaactg acattaagcg ttcattataa ccaattaatt ttgatttggt
     7201 tgataacgaa ctttattaat aacaaatata gaaaaagtac ccatactttt atctcctttc
     7261 catttttttc attatgaatg agataaagta atttctacta ctgcgatact ggtgcacata
     7321 attgaaacct ccttatgtaa ctgaatatga ttgtatcatc ttttcatttt agaataaacc
     7381 tttatatgta attttgcact tgacacttca gatggacgtt ttcttcaaaa taatatgttt
     7441 tgaaacttga taagagaagc cactgtcttt actaaaaagt aaaggctgcg ataaaggaca
     7501 gtgactttaa taaagttgca ctgttgtatt gtctttggat aaaattgtct aaagggaagt
     7561 tttatcccca acgaaaaaac atctaaagtg gtaagtaatg aaagaatgtc gataaagtag
     7621 gaatggggga tctttttgaa tacaatagaa tcacagtttg ataaggttgc agaagattac
     7681 gatttcgtga atgagctttt gaatgattat tcattctttg tgtctaatat gtctccaaag
     7741 aaaggcagag cattagatat cggatgtggc tcgggtttgt tagtggagaa attagcaagt
     7801 tattatgatg aagtggtagg gattgatatt tctaatcaaa tgctcgatct tgccaaatct
     7861 aaacgtcaac taacaaatac ggtctatctg aatatgaatg cagaacaact taattttaat
     7921 gagaagtttg attttattgt aagccgaaca acctttcatc atttggatga tatagccagc
     7981 gtaatacagc aaatgaagga actgttgaat gaggaaggaa gaatagttat tcttgataat
     8041 gtatctgaag tcgagacacc gcctacctat gtttataaat taggggcaat ccaagagttt
     8101 ttaccgcatt gtttcaaatt tggaataaaa aatgcgatta gaatttataa tcacaacaca
     8161 tctaaatcat ggcttgagca tttagcttca gataagtacc tatctgaaca aaattattac
     8221 gatttatatg aaaagttgtt gcctggatgc caattccata aaatgggttg ggcaatgggg
     8281 gttgtctgga caaaataact taaagctgtg gctttgaaaa gttgaatttg tgattgtttt
     8341 cagagctatc gggtgcttta gtataaataa caatgaataa aaaatcagcc tgtatcttat
     8401 gttactatta agtaatttaa gatacaggtt ttactatttt tgaaataagt accttccact
     8461 tcaaatacat gtattcaaag tgaaagtatt gatttaaatg attcttttgt aatttactct
     8521 tgataagatg taacgccctc acaagaagat atcataacaa caatgaggtt gaaggagtgt
     8581 ggtttgattt tagggataga tttattggat catattataa tcggtgataa tagatttacc
     8641 agtcttgtag aagcgggtta ctttgatgaa aatgattgaa gttgatagaa ttaatgacgt
     8701 atcttgtgta taatacctac gaagtacttt cattggagga aaaatagtga ctctatttat
     8761 tattatcggg gttctcgtgc caatggttta taccatgcag ttaaatatta aaaatgaacc
     8821 tgtaacaaag cgcaatctat taataacatt agctttatct acgttaggta ttttagtaac
     8881 ggcgttagca ggtgtgatcg ttacgaaaca agcttttcct ttattaagtg tagcaatcgg
     8941 ctcaattttt actggaatcg tttggggcct tttactaagt ggtagctacg cgctgatacg
     9001 atttttatct aacgcatttg ggcgtaagta atagtaatct gttcatcaag tagtatccgt
     9061 gcttgaaacc aaattaaaac tcctaatgtg gaactagtta aaaaattcta aactagtaca
     9121 ttaggagttt tgttatgcag aataaattta attgttaaat tgaaagtgcg gtaaaaaccc
     9181 actatttatt tgaaaaaaat cgagcaacca aattaaacct tgatatccta agtaaataca
     9241 taataaaaca agtccgacat aaattaaaaa tcgcaaaata gaaagtccaa ctctaaaaag
     9301 gaggatgact aatagtgcta ttaaaataat tgttagtata ctcaatgcta caaacctcct
     9361 catacgcttt taaatcataa caaaattata agctatttta aatgaacgtg catcagtcct
     9421 aagatatatc atgattaagg ctgaagactg aggaaaatat acataataaa atatataatt
     9481 agatgaagac attggaggcg aactatgaga ttgatattta gtatgattaa aaacattata
     9541 gcagtaatag caattgtgat aattgtttat atcgccttga aatacgcacc ttttttaaaa
     9601 gaacaagatt ggaatcctgt aaatcacgat aatgatcaaa tgaatcaagt tacgcaaccc
     9661 actaatgatg cccaacatgt ttatgtaagt ggtgagaaat attcattaaa agaaaatgat
     9721 ttaataaaga atgtaccatt aagccaaatt aaaaatgtat ttaaaatgat agataaacaa
     9781 gagtttatgg ctgtgtctgg aatgaatcgc atggcttata atgatcaata tattataggt
     9841 caaagaggag acgaatttat tctttataaa tttggagatg agtcaatgcg tgtttacaat
     9901 actgaatttg aaatgcaaca agacttaaat gaattagggc aaaatttaca actaaaaccc
     9961 gaaaatgctt atcaatagga aattattgca taagaaatat attttagtta tcctgtcact
    10021 agaataaatt taaaaggttg gtgtagttca aatggcagaa caatcaaaac agaaacaagc
    10081 taatgaacaa caaaaggccc agaacttatt cgcacgttgg agacaacttc agaattcgaa
    10141 cagtgaatcc tcgaacgata caaacaaata attttaaaat ttaaatcgat tatttaaact
    10201 actagtgact ggaatgtttg ctagtagttt tttgttttta aaatgccaca aattttttac
    10261 ggagttgttc attttcaaag taattcgtag ttagtgcttt atttcataat gggagtaata
    10321 taaaatagaa aaggatatac aatattttga cataatagag gtgttctggg tgcttaagtt
    10381 ttttaaaaat aacaaattaa ttgttgtttt atgtgcaatt atcgttttta ttgcattaat
    10441 tgggctgtcc atacgttcac aatctcaatc acctcctgaa caatatatag gtgattctgt
    10501 gtcttttgga caacgagttg tgagttatcc agttaatttt gttgctggta cgattgggga
    10561 cttttttaaa aaaggagatt ctaaagaatc taaaaataag attagccagt tagaatctaa
    10621 gaaccaacaa ttagaagcgg aaaatgaaaa attaaaaaaa gagcttgatt taaaagatat
    10681 ttcaaaattt gatcctattt ctactacggt tttagcaaga aatccggatc agtggatgaa
    10741 tacaattgta attgataagg gatctaaatc aggtataact tcaaatatgg ctgtgatgac
    10801 atcacaaggt tttgttggaa gagttactaa agttaataaa ttttcttcac aagttgattt
    10861 aatctcaact aatacacgtg cgggtaaatt atctgtaaat atacaacacg gttctaaaaa
    10921 tatatttggt ttaattgatc gttatgatga aaagaactca gaacttgtaa ttagtgacat
    10981 taataataga gataatatct caaaaggtga taaagtcgtt acaagtggat tagctgatca
    11041 actaccaagt aatttatata taggagaagt gactaaggtt caaaatgatc aatacggctt
    11101 agctaaagaa gttagggtta agactggtgc agacttaaca gatttgagtc atgtttatgt
    11161 tgcaaaaaga gatcctaaaa caattcctga tgatgaaagc agggataaat aatgcgtaca
    11221 ctgtattatt ttttgatagg tattttacta ttttatatag atactgcaat tgggcttctt
    11281 attccaatgc atataggtaa gtttgaactt gtatttgtac cacaccttac atttatgtat
    11341 attttaatga tggtagtgta tcgtggcttt ggcgtatcat tattgctcag catattttta
    11401 ggcgtaatga ctgatgtata ctttggtagt atttatggag tgtacttatt tggctatata
    11461 ttgtttttag cacttataga tcgattcttt aaaatttttt acaaagatca ttcgatgtta
    11521 ttcattatta tattagccag caccttatta ttagaagtct atgtggcatt aatatacggt
    11581 atgttaggat tcattcaatt tgatattatt cattttgtag tctttagatt attgccaaca
    11641 ttaattatga attttgtact gttgattatg ctttatccgt tgattataaa gttccttaaa
    11701 aaaacaaaca atgacattga catgaaacgt cgtcaatggt aagatgcaat agttgagtag
    11761 ttataagtag ctacatacaa ccgctcaaat ataggttcaa gaacattttt aatgaagaaa
    11821 gcgattgtca atcaaggcgt tgaaggaaga aacgagaaga gtttatgttt ataaatgact
    11881 cgagttgcga ccgaaacaac gtagagtgca aacgcttgac tcaataacga aatgtcacct
    11941 atgtatggcg tgacttatta tacaggaggt gcaaagtatg tttgctatta ttgaaacagg
    12001 tggaaaacaa atcaaagtag aagaaggtca agaaatcttc gttgaaaaat tagacgtaaa
    12061 cgaaggagat acttttacat ttgataaagt attatttgta ggtggagatt cagttaaagt
    12121 tggagcgcca acagttgaag gtgcaacagt tactgctact gttaataaac aaggtcgcgg
    12181 taaaaaaatc actgtattca catacaaacg tcgtaaaaat tcaaaacgta aaaaaggcca
    12241 tcgtcaacca tacactaaat taacaatcga taaaatcaac gcgtaattat tatgattact
    12301 gttgatatta cagttaatga tgaaggcaaa gtaacagacg ttattatgga tggccatgct
    12361 gaccatggtg aatatggtca tgatatcgtt tgtgctggag cttcagctgt attgtttggt
    12421 agtgttaatg cgattatagg attgacatct gagagaccag atatcaatta tgacgacaat
    12481 ggtggtcatt ttcatataag aagcgttgat acaaataacg atgaagcgca actaattctt
    12541 caaacaatgc ttgtgtcttt acaaactatt gaagaagaat ataatgagaa tattagatta
    12601 aattataagt gaggtgcatt ccgatgttaa aattaaactt acaattcttc gcatctaaaa
    12661 aaggggtaag ttctacaaaa aacggacgtg actctgaatc aaaacgctta ggtgctaaac
    12721 gtgctgacgg tcaattcgta acaggtggtt caattttata tcgccaacgt ggtactaaaa
    12781 tttaccctgg tgaaaatgta ggtcgtggtg gcgatgatac attattcgct aaaatcgacg
    12841 gcgttgttaa attcgaacgt aaaggtcgcg acaaaaaaca agtttctgta tatgcagtag
    12901 ctgaataatt ttgtctagtt aacaccagaa gtgaatcttc tggtgttttt tactt
//
LOCUS       DANBWH010000064        12831 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.066, whole genome shotgun sequence.
ACCESSION   DANBWH010000064 DANBWH010000000
VERSION     DANBWH010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12831
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.066"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..370)
                     /locus_tag="PPY92_001247"
     CDS             complement(<1..370)
                     /locus_tag="PPY92_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HDJ2625259.1"
                     /translation="MAKINFDAATKGNPGISTCAIVIKEDKQHYTYTHELGEMDNHTA
                     EWAACIYALEHARELNVQNALLYTDSKLIADSIEAGYVKNANFKPYFDQIEIFEKDFD
                     LLFVKWIPREQNKEANQHAQQ"
     gene            complement(853..1557)
                     /locus_tag="PPY92_001248"
     CDS             complement(853..1557)
                     /locus_tag="PPY92_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HDJ2625260.1"
                     /translation="MYNEILGLVTFIATFVLMVLMYRFFGKQGLIAWVAIGTIIANIQ
                     VIKTVEIFGISATLGNVMFASIYLATDILNDIYGRRVAKRAVWLGFSSTLIMIIVMQL
                     SLHFIPAPEDMAQKALHAIFDVVPRIALGSIVAYIIGQHIDVFIFSLIKKVFSSDKTF
                     FIRAYGSTFLSSIIDTALFVAIAFIGSLPGTVVFEIFITTYVLKLASTVFNVPFGYIA
                     KSFYRKGKIDKLDQGY"
     gene            1802..1996
                     /locus_tag="PPY92_001249"
     CDS             1802..1996
                     /locus_tag="PPY92_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HDJ2625261.1"
                     /translation="MSRILTFSEQNTLAKIDDLMNTYCNQCPIKTRLRKLEGKTKAHH
                     FCINECSIGKEIKQLGNELQ"
     gene            2008..2259
                     /locus_tag="PPY92_001250"
     CDS             2008..2259
                     /locus_tag="PPY92_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HDJ2625262.1"
                     /translation="MKIISISETPNHNTMKITLSESREGMTSDTYTKVDDSQPAFIND
                     ILKVEGVKSIFHVMDFISVDKENDANWETVLPKVEAVFE"
     gene            2297..3421
                     /locus_tag="PPY92_001251"
     CDS             2297..3421
                     /locus_tag="PPY92_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor C"
                     /protein_id="HDJ2625263.1"
                     /translation="MEILRIEPTPSPNTMKVVLSYTREDKLSNTYKKVEETQPRFINQ
                     LLSIDGITSIFHVMNFLAVDKAPKADWEVILPDIKAAFSDANKVLESVNEPQIDNHFG
                     EIKAELLTFKGIPYQIKLTSADQELREQLPQTYVDHMTQAQTAHDNIVFMRKWLDLGN
                     RYGNIEEVMDGVLEEVLATYPESQLPVLVKHALEENHATNNYHFYRHVSLDEYHATDN
                     WKTRLRMLNHFPKPTFEDIPLLDLALSDEKVPVRRQAIVLLGMIESKEILPYLYKGLR
                     DKSPAVRRTAGDCISDLGYPEALPEMVLLLDDPQKIVRWRAAMFIFDEGNAEQLPALK
                     AHINDNAFEVKLQIEMAISRIENGDEALGSVWKQMANRTI"
     gene            3437..3874
                     /locus_tag="PPY92_001252"
     CDS             3437..3874
                     /locus_tag="PPY92_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HDJ2625264.1"
                     /translation="MNAYDAYMKEIAQQMRGELTQNGFTSLETSEAVSEYMNQVNADD
                     TTFVVINSTCGCAAGLARPAAVAVATQNEHRPTNTVTVFAGQDKEATATMREFIQQAP
                     SSPSYALFKGQDLVYFMPREFIEGRDINDIAMDLKDAFDENCK"
     gene            4298..5254
                     /locus_tag="PPY92_001253"
     CDS             4298..5254
                     /locus_tag="PPY92_001253"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDJ2625265.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYNGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            5454..5933
                     /locus_tag="PPY92_001254"
     CDS             5454..5933
                     /locus_tag="PPY92_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDJ2625266.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            5948..6787
                     /gene="fakB2"
                     /locus_tag="PPY92_001255"
     CDS             5948..6787
                     /gene="fakB2"
                     /locus_tag="PPY92_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HDJ2625267.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQA
                     SQMVDANVTVIDSKSISFSLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSIGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            6873..7406
                     /gene="msrA"
                     /locus_tag="PPY92_001256"
     CDS             6873..7406
                     /gene="msrA"
                     /locus_tag="PPY92_001256"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDJ2625268.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHVDNPTYE
                     QVCTNKTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DEHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            7399..7827
                     /gene="msrB"
                     /locus_tag="PPY92_001257"
     CDS             7399..7827
                     /gene="msrB"
                     /locus_tag="PPY92_001257"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDJ2625269.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMVRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            7839..8339
                     /locus_tag="PPY92_001258"
     CDS             7839..8339
                     /locus_tag="PPY92_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDJ2625270.1"
                     /translation="MFKKLFGKGKEVQKDIAIYAPLTGEYVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEFINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
                     IDVTMN"
     gene            8339..8560
                     /locus_tag="PPY92_001259"
     CDS             8339..8560
                     /locus_tag="PPY92_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HDJ2625271.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDDLYEEYTEWLKF"
     gene            8752..10242
                     /locus_tag="PPY92_001260"
     CDS             8752..10242
                     /locus_tag="PPY92_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HDJ2625272.1"
                     /translation="MDDKQHTSSSDDERAEIATSNQDQETNSSKRVHLKRWQFISILI
                     GTTLITAVITVVAYIFINQKISGLNKTDQANLNKIENVYKILNSDYYKKQDSDKLSKA
                     AIDGMVKELKDPYSEYLTKEQTKSFNEGVSGDFVGIGAEMQKKNDQIMVTSPMKGSPA
                     ERAGIRPKDVITKVNGKSIKGKALDEVVKDVRGKENTEVTLTVQRGSEEKDVKIKREK
                     IHVKSVDYKKKGKVGVITINKFQNDTSGELKDAVLKAHKDGLKKIVLDLRNNPGGLLD
                     EAVKMANIFIDKGKTVVKLEKGKDTEAIQTSNDSLKEAKDMDISILVNEGSASASEVF
                     TGALKDYNKAKVYGSKTFGKGVVQTTREFKDGSLLKYTEMKWLTPDGHYIHGKGIKPD
                     VTIDTPKYQSLNVIPNTKTFKVGDDDKNIKTIKIGLSALGYKVDNESTQFDQALENQV
                     KAFQQANKLEVTGEFNKETNNKFTELLVEKANKHDDVLDKLINILK"
     gene            10642..11151
                     /locus_tag="PPY92_001261"
     CDS             10642..11151
                     /locus_tag="PPY92_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDJ2625273.1"
                     /translation="MIRLGKMSDLDQILNLVEEAKELMKEHDNEQWDDQYPLLEHFEE
                     DIAKDYLYVLEENDKIYGFIVVDQDQAEWYDDIDWPVNREGAFVIHRLTGSKEYKGAA
                     TELFNYVIDVVKARGAEVILTDTFALNKPAQGLFAKFGFHKVGEQLMEYPPYDKGEPF
                     YAYYKNLKE"
     gene            11163..12233
                     /locus_tag="PPY92_001262"
     CDS             11163..12233
                     /locus_tag="PPY92_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDJ2625274.1"
                     /translation="MTKIAFTGGGTVGHVSVNLSLIPTALSQGYEALYIGSKNGIERE
                     MIESQLPEIKYYPISSGKLRRYISLENAKDVFKVLKGILDARKVLKKEKPDLLFSKGG
                     FVSVPVVIAAKSLNIPTIIHESDLTPGLANKIALKFAKKIYTTFEETLNYLPKEKADF
                     IGATIREDLKNGNAHNGYQLTGFNENKKVLLVMGGSLGSKKLNSIIRENLDALLQQYQ
                     VIHLTGKGLKDAQVKKSGYIQYEFVKEDLTDLLAITDTVISRAGSNAIYEFLTLRIPM
                     LLVPLGLDQSRGDQIDNANHFADKGYAKTIDEEQLTAQILLQELNEMEQERTRIINNM
                     KSYEQSYTKEALFDKMIKDALN"
     gene            12250..>12831
                     /locus_tag="PPY92_001263"
     CDS             12250..>12831
                     /locus_tag="PPY92_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDJ2625275.1"
                     /translation="MSQWKRISLLIVFALVFGIIAFFHESRLGKWIDNEVYEFIYSSE
                     SFITTSIMLGATKVGEVWAMLCISLLLVAYLMLKRHKIEALFFALTMALSGILNPALK
                     NIFDRERPTLLRLIDITGFSFPSGHAMGSTAYFGSGIYLLNRLNQGNSKGILIGLCAA
                     MILLISISRVYLGVHYPTDIIAGIIGGVFCIILS"
BASE COUNT         4724 a         1852 c         2263 g         3992 t
ORIGIN      
        1 cctgttgagc gtgttgattc gcttctttgt tttgttctct cggtatccat ttaacaaata
       61 ataaatcaaa atctttttca aatatttcta tttgatcaaa ataaggtttg aaatttgcgt
      121 ttttcacata accagcttca atgctatctg caattagctt tgagtctgta tataatagtg
      181 cgttttgaac atttaattca cgtgcatgtt ctagtgcata aatacatgca gcccattctg
      241 cagtgtggtt atccatttcg cctaactcat gtgtatatgt ataatgctgc ttatcttctt
      301 tgattacaat ggcacatgta cttatgcctg gatttccttt cgtcgcagca tcaaaattta
      361 ttttcgccat aataaaccta ctttctattc aatacttagt taaagttact attactgtaa
      421 tacaaaatat gttgggtaat ccattaaaaa acacgcatca cttaaataag taacacgtgt
      481 ttaaaatact cgctgattca aagatgattt tctaatacgt agtactgtaa tatacttcct
      541 aaaaaaatca tcttcaggct gggacataaa tcaatgttct atgctctacg aagttatatt
      601 ggcagtagtt gactgaacga aaatgcgctt gtaacaagct tttttcaatt ctagtcaggg
      661 gccccaacac agaagctgac gaaaagtcag cttacaataa tgtgcaagtt ggggatggac
      721 cccaacaaag agaaattgta ttcccaattt ctacagacaa tgcaagttgg ggtgggacga
      781 cgaaataaat tttgcgaaaa tatcatttct gtcccactcc cttaaaactt attcttttgt
      841 gtagtaagtg cgttaatagc cttgatctaa cttatcaatc ttacctttac gataaaatga
      901 tttagcaata tatccaaatg gtacattgaa aactgttgaa gctaatttta atacgtaagt
      961 tgtaataaat atttcaaata caactgtacc aggtaaactt ccgataaaag cgatagctac
     1021 aaataaagct gtatcaatta ttgagcttaa aaatgtacta ccatatgcac ggatgaaaaa
     1081 cgttttatca gaactaaata cttttttaat tagtgaaaag ataaatacat caatatgttg
     1141 accaataata tatgcgacaa ttgagcctaa agcaatacgt ggcacaacat caaagattgc
     1201 gtgtaatgct ttttgtgcca tatcttctgg tgcaggaata aaatgtaatg acaattgcat
     1261 aacaataatc ataattaatg ttgatgaaaa gcctaaccaa actgctcttt ttgcaactct
     1321 acgcccataa atatcattta aaatatctgt tgctaaataa atagaagcaa acatgacatt
     1381 acctaaagtt gctgaaatac caaagatttc tacagtttta atcacttgta tgttggcaat
     1441 gattgtgcca attgcaaccc atgcaattaa accttgttta ccaaaaaagc gatacataag
     1501 taccataagt acgaacgttg caataaacgt aactagtcct aaaatttcat tatacatatt
     1561 aaaatgtcct cctaaatttt gatcatgcgg gtgtttagaa accgctcaat aaataactta
     1621 ctcattttac tgaaaaatcg tttgcgatgc aatagcattt gaatataaat acattttacg
     1681 tatagaattg taaaaggttt cattcaaatc ttagggtcaa aaatgttata atatttttat
     1741 gtcaaattta aaacagtaac acttatttac aaggttgcaa tattttgaag taataaagga
     1801 agtgtcgcgt attttaactt tttcagagca aaatacactc gcgaaaatag atgatttaat
     1861 gaatacttat tgcaatcaat gtccaatcaa aactcgtctg cgtaaattag aggggaaaac
     1921 gaaggcgcat catttttgta tcaatgagtg ttcaataggg aaagaaataa aacaattagg
     1981 aaatgaactt caataggagg aagtcaaatg aaaattatat ctatatcaga aacaccgaac
     2041 cacaacacaa tgaagattac acttagtgaa agcagagaag gtatgacatc agatacgtat
     2101 actaaagttg atgattcaca gccagcattt attaatgaca tcttaaaggt tgaaggcgtt
     2161 aaatcaattt tccatgttat ggactttatt tcagtagata aagaaaatga cgcaaattgg
     2221 gaaacagtat tgccaaaagt agaggctgta ttcgaataaa tttttcatca actagtattc
     2281 ggggggaata aagtatatgg aaattttacg tatagagcca acaccaagtc caaatacaat
     2341 gaaagttgtt ttgtcatata caagagaaga caagttatct aatacttata aaaaagtaga
     2401 agaaacacaa ccaagattta taaatcagtt gttatctata gatggtatca cttccatttt
     2461 tcatgtcatg aacttcttag ctgttgataa ggcaccaaaa gctgattggg aagtcatatt
     2521 acctgatatt aaagctgctt tttctgatgc gaataaggtt ttagaatctg taaatgaacc
     2581 tcaaattgac aatcattttg gtgaaattaa agctgaatta ttaactttta agggtatacc
     2641 gtatcaaatt aagctaactt ctgctgacca agaattaaga gaacaattac cacaaacata
     2701 tgttgatcat atgactcaag cgcaaacagc acatgacaat attgttttta tgcgtaaatg
     2761 gctagattta ggaaatcgct atggaaatat tgaagaagta atggatggtg tcctagaaga
     2821 agtgctagct acctatccag aatcacagtt acccgtattg gtaaaacatg ctttagaaga
     2881 aaatcacgca actaataatt atcatttcta tcgacatgtc tctttggatg aatatcatgc
     2941 aactgataat tggaagactc gattacgaat gttaaaccat tttccaaagc cgacttttga
     3001 agatataccg ctacttgatt tagctttatc tgatgaaaaa gtaccggtta gacgtcaagc
     3061 gattgtatta ttaggtatga ttgaaagtaa agaaatttta ccgtatttat ataaggggct
     3121 tcgtgataaa agtcctgctg taagaagaac agcaggggat tgcataagcg atttagggta
     3181 tccagaggca ctaccagaaa tggtgctact attagatgat ccacagaaaa tcgttaggtg
     3241 gcgtgctgct atgtttatct ttgatgaagg taatgcagag cagcttcccg cactaaaagc
     3301 ccatattaat gacaatgcgt ttgaagttaa attacaaatt gaaatggcca tatctcgtat
     3361 tgaaaacgga gatgaggcac ttggttcagt atggaaacaa atggcaaaca gaacaattta
     3421 attggaggaa ttaaatatga atgcatatga tgcttatatg aaagaaattg cgcaacaaat
     3481 gcgtggcgaa ttaactcaaa atggttttac aagtttagaa acgagcgaag cagtatcgga
     3541 gtatatgaac caagtaaatg ctgatgacac tacttttgta gttattaact ctacatgcgg
     3601 ctgtgcagct ggattagcaa gaccagctgc agtagcagtt gcaacacaaa atgaacatag
     3661 acctacaaat acagttacag tttttgctgg gcaagataaa gaagcaactg ctacaatgcg
     3721 agaattcatt cagcaagcac catctagtcc ttcgtatgct ttattcaaag gtcaagattt
     3781 agtttatttt atgcctagag aatttatcga aggtagagat attaatgaca ttgcaatgga
     3841 cttaaaggat gcctttgacg aaaattgtaa atagtacaca taaataaata taaaggttaa
     3901 cacattttat aatattaaaa atggtgtctg tcattgaaaa tagagaatat agttgtattc
     3961 tatttgttaa ataaagtccg tttttaccaa ctatattttc tagaaattta actgttttaa
     4021 taggacatca aacataatat tcaaatcatg tgttaacctc ttttttaaaa ttttttagca
     4081 ttaaagttat agatttgggt aaacaattac caattggaaa catatatcac gttacgatgt
     4141 ggtaggtact taatcagcat tttataaata aagtaacgga attcatgata ttaatatcat
     4201 attcctaaaa tgagtgataa caaaatgcta cataaaatta agttatatca aactaaatat
     4261 acatactata aataatgaaa atgaggtgtt atcgcatatg ttgaattcat ttgatgcagc
     4321 atatcacagt ctttgtgaag aggttttaga aataggaaat acacgaaatg atcgcacaaa
     4381 tacaggtacg atttcgaaat ttggtcatca acttcgcttt gacttatcta aaggatttcc
     4441 actattaacg acaaagaaag tttcttttaa attagtagca accgaattat tatggttcat
     4501 taaaggagat acaaacatcc aatacttatt aaaatataat aataatattt ggaacgaatg
     4561 ggcttttgaa aattatataa aatcagacga gtataacggt ccagatatga cagatttcgg
     4621 gcatcgtgca ttgagtgatc ctgaatttaa cgaacaatat aaagaacaaa tgaaacaatt
     4681 taagcaacgt attcttgaag atgatacttt tgcgaagcaa ttcggtgatt taggaaatgt
     4741 ttatggtaaa caatggcgag attgggttga taaagatggt aatcattttg atcaacttaa
     4801 aacagtaatt gaacaaatta agcataatcc agattcaaga cgacacatcg tatctgcatg
     4861 gaatccaaca gaaattgata caatggcact tccgccttgt cataccatgt tccagtttta
     4921 tgtccaagat ggtaagttaa gttgccagtt ataccaacgt agcgcagata tctttttagg
     4981 tgtgccattt aatatcgcaa gctacgcttt attgacacac cttattgcca aagaatgtgg
     5041 acttgaagta ggagaatttg tgcatacatt tggagatgca catatttatt caaatcatat
     5101 tgatgcgatt caaacacaat tagcacgtga aagcttcaat cctccaacat taaaaattaa
     5161 cagtgacaag tctattttcg acataaatta tgaagatttg gaaattgttg actatgaatc
     5221 acatccagca ataaaagctc caatagcagt gtagtcattg catagttagc taaccatata
     5281 gacatcaaaa tgacatcata gtattttcaa gtgcaaaaaa gtactttttt gtgttaaacg
     5341 ttttcataaa ttatgcaaaa tcattatttc tatcacactt tatgataaaa attgtgttaa
     5401 attaaagata acttagtaat aaaaaatgaa atgatagaag aaggaggata attatgactt
     5461 tatccattct agttgcacat gacttgcaac gagtaattgg ttttgaaaat caattacctt
     5521 ggcacctacc aaatgatttg aaacatgtta aaaaattatc aacaggtcat actttagtaa
     5581 tgggtcgtaa gacatttgaa tcgattggta aaccactacc gaatcgtcga aatgttgtac
     5641 ttacttcaga tacaagtttc aacgtagagg gcgttgatgt aattcactct attgaagata
     5701 tttaccaact accgggccat gttttcatat ttggagggca aacattattt gaagaaatga
     5761 ttgataaagt ggacgacatg tatattactg ttattgaagg taaattccgt ggtgatacgt
     5821 tctttccacc ttatacattt gaagactggg aagttgcctc ttcagttgaa ggtaaactag
     5881 atgagaaaaa tacaattcca catacctttc tacatttaat tcgtaaaaaa taagggggaa
     5941 aacgaccatg acaaaacaga ttatagtaac agactcaaca tccgatttat ctaaagaata
     6001 cttagaagca aacaacattc atgtaattcc tttaagttta actattgaag gagcttcata
     6061 cgttgaccaa gtagatatta catcagaaga atttattaat catattgaaa atgatgaaga
     6121 tgtaaagaca agtcagccag ccataggtga atttatatct gcttatgaag aactaggaaa
     6181 agatggctct gaaatcataa gtattcatct ttcttcagga ttaagtggta catataacac
     6241 tgcttaccaa gcaagtcaaa tggtagatgc taatgtaact gttattgatt caaaatctat
     6301 ttcttttagt ttagggtatc aaatacaaca cctagtagag cttgtaaaag aaggtgtctc
     6361 aacttctgaa atagttaaaa agttaaatca tttaagagaa aacattaaat tatttgtagt
     6421 tatagggcaa ttgaatcaat taattaaagg tggcagaatt agtaaaacaa aaggtttgat
     6481 tggtaatctt atgaaaatta aaccaattgg tacacttgat gatggtcgct tagagcttgt
     6541 gcacaatgcg agaactcaaa attctagtat ccaatacttg aaaaaggaaa ttgctgaatt
     6601 tataggagat catgaaatca aatccattgg tgtcgcacat gctaacgtca ttgaatatgt
     6661 tgataaattg aagaaagttt ttaacgaagc ttttcatgtg aataattacg atataaatgt
     6721 aactacacca gttatttctg cacatactgg tcaaggtgcg attggcctcg tagtccttaa
     6781 gaagtaaatt taatcttttc agtgttaatt acttccattt caatccttta tagactaaat
     6841 ttataattag atagatagag gaggtaattc atatgacaaa agaatatgca acattagcag
     6901 gaggatgttt ctggtgcatg gttaaaccat ttacatcata tccaggcatc aagtcagtcg
     6961 tatctggtta tagtggcggt catgttgata accctactta tgaacaggta tgtacgaata
     7021 aaaccggcca tgtcgaagca gtacaaatta cgtttgatcc agaggttact tcctttgaaa
     7081 atatattaga catatatttc aaaacatttg acccaactga tgatcaaggg caatttttcg
     7141 atagaggcga aagctatcaa ccagtcattt tctatcatga tgagcatcag aaaaaggctg
     7201 ctgagtttaa aaagcaacaa ttaaatgaac aaggtatttt caagaaacca gtgattacac
     7261 ctattaaacc atataaaaat ttctatccag ctgaagacta ccatcaagat tattataaaa
     7321 agaacccggt acattattac caatatcaac gtggttcagg tagaaaagcg tttatagaat
     7381 cacattgggg gaatcaaaat gcttaaaaaa gataaaagtg aactaacaga catagaatat
     7441 attgttacac aagaaaatgg cactgaacca ccatttatga atgaatattg gaatcatttt
     7501 gctaaaggga tttatgtaga taagatttct ggtaaacctt tatttacatc tgaagaaaag
     7561 tttcattctg aatgtggatg gcctagcttt tctaaagcgc ttgatgacga tgaaattata
     7621 gaattagtcg acaaatcatt tggtatggta agaactgaag tgcgttcaga agaatcaaat
     7681 agtcatttag gacacgtctt taatgatgga cctaaagaaa gtggcggctt aagatactgt
     7741 atcaattccg ctgcaattca atttattcca tatgaaaaat tagaagaatt gggttatggc
     7801 gatttaatat cacattttga taagtaggag tggaaagtat gtttaaaaaa ttattcggaa
     7861 aaggcaaaga agttcagaaa gatatcgcaa tttatgcacc attaactgga gaatacgtga
     7921 aaattgaaga tattccagat cctgtattcg cacaaaaaat gatgggcgaa ggttttggta
     7981 ttaatccaac tgaaggagaa gttgtgtctc caattgcagg acgtgttgac aatgtctttc
     8041 caactaagca tgcgattggg ttaaaagcag ataacggatt agaattatta gttcatatcg
     8101 gtttagacac agttcaatta gacggtgaag gatttgaagt gttagtgtct agtggtgacg
     8161 aagttaacgt cggagatcca ttagtaagat tcaaccttga atttattaat aataacgcta
     8221 aatctgttat ttcaccaatt ataattacta acactgatca agcagcttca attaatattt
     8281 atgatgaaaa tgctgtgatt aaaggtgaaa caaaagtgat tgatgtgaca atgaactaat
     8341 gaaaaactat tcgttttatc aatttgtcat gacagttcgt ggtcgacacg acgataaagg
     8401 tcgtctagca gaagagatat ttgacgatct tgctttccca aaacacgatg atgattttaa
     8461 catactgtct gattatattg agacacatgg tgatttcaca ttgccaatgt ctgtatttga
     8521 tgatttatat gaagaatata cggaatggct aaaattttaa tataattttt aaaaataata
     8581 gttagaacca gggtgatgca attcgttatc ctggttttaa tttaaaataa actaagttgt
     8641 gactaaaaat taatcaatta tagtgaaata tggtgcgcta tcttgcataa attgatatga
     8701 ttaactacac agaatttaaa agtacataat acataataag gaagtgatac aatggatgat
     8761 aagcaacaca catcttcatc cgatgatgaa cgcgctgaaa ttgcaacaag caatcaagac
     8821 caagaaacta attcatcgaa acgcgttcac ttaaaacgtt ggcaattcat atcaatatta
     8881 attggcacaa ccctaataac agctgtcatc acagttgttg catatatttt tataaatcaa
     8941 aaaataagtg gtttaaacaa aactgatcaa gcaaacttaa ataaaattga aaatgtgtat
     9001 aaaatcttaa atagtgatta ttacaaaaaa caggactctg acaagttaag taaagctgca
     9061 attgatggta tggtcaaaga attaaaagat ccttattctg aatatttaac aaaagaacaa
     9121 acgaaatcct ttaatgaagg tgtttcaggt gattttgtag gtattggtgc agaaatgcaa
     9181 aagaaaaatg atcaaattat ggttactagc cctatgaagg gttctccagc agaacgtgct
     9241 ggcatccgtc ctaaagatgt cattactaaa gtaaatggaa aatcaattaa aggaaaagca
     9301 ttagacgaag ttgtcaaaga cgttcgtggt aaagaaaaca ctgaagtaac tttaactgtt
     9361 caacgaggca gtgaagaaaa agacgttaag attaaacgtg aaaaaattca tgttaaaagt
     9421 gttgattata agaaaaaagg taaggttgga gttattacta ttaataaatt ccagaatgat
     9481 acatcaggtg aattgaaaga tgcagttctt aaagctcaca aagatggttt gaaaaagatt
     9541 gttttagatt taagaaataa tcctggtgga ctactagatg aagctgttaa aatggcaaat
     9601 atttttatcg ataaaggaaa aactgttgtt aaactagaaa aaggtaaaga tactgaagca
     9661 attcaaactt ctaatgactc gttaaaagaa gcgaaagaca tggatatatc catcttagtg
     9721 aatgaaggtt cagctagtgc ttctgaagtg tttactggtg cgctaaagga ctataataaa
     9781 gctaaagttt atgggtcaaa aacattcggc aaaggtgtcg tacaaactac aagagagttt
     9841 aaggatggtt cattgttaaa atatactgaa atgaaatggt taacgccaga tggtcattat
     9901 attcacggta agggcattaa accagacgtt actattgaca cacctaaata tcaatcttta
     9961 aatgtcattc ctaatacgaa aacatttaaa gttggagacg atgataaaaa tattaaaact
    10021 attaaaattg gtttatcagc tttaggttat aaagttgata atgaatcaac gcaatttgat
    10081 caagctttag aaaatcaagt taaagctttc caacaagcga ataaactcga ggtaactggt
    10141 gaatttaata aagaaacgaa taataaattt actgagttat tagttgaaaa agctaataaa
    10201 catgatgatg ttctcgataa gttgattaat attttaaaat aagcgataca cactactaaa
    10261 attgtattat tattatgtta atgacacgct tcctaaattt gcaaagatag caatttagga
    10321 ggcgtgttta tttttattga cgtctaaccc taaaagatat aaattagaca tttacaaatg
    10381 atgtaaataa cgcaatttct atcatcgctg ttaacaattc atgctttaat atactatgag
    10441 catatacttt ttaaatagta ttattcacta gttttaacaa tcgattaatt ggtatatgat
    10501 acttttattg gttattttca tcccatagtg tgataattac tatttttcat tcataataaa
    10561 ggtttaaagc atgttaatag tgtgtaagat taacatgtac tgaaaaacat gtttaacata
    10621 acgatataag gagtgacgta catgatccgt ctaggtaaaa tgtcagattt agatcaaatc
    10681 ttaaatctag tagaagaagc aaaagaatta atgaaagaac acgacaacga gcaatgggac
    10741 gatcagtacc cacttttaga acattttgaa gaagatattg ctaaagatta tttgtacgta
    10801 ttagaggaaa atgacaaaat ttatggcttt attgttgtcg accaagacca agcagaatgg
    10861 tatgatgaca ttgactggcc agtaaataga gaaggcgcct ttgttattca tcgattaact
    10921 ggttcgaaag aatataaagg agctgctaca gaattattca attatgttat tgatgtagtt
    10981 aaagcacgtg gtgcagaagt tattttaacg gacacctttg cgttaaacaa acctgcacaa
    11041 ggtttatttg cgaaatttgg atttcataag gtcggtgaac aattaatgga atatccgcca
    11101 tatgataaag gtgaaccatt ttatgcatat tataaaaatt taaaagaata gaggtaatat
    11161 taatgacgaa aatcgcattt accggagggg gaacagttgg acacgtatca gtaaatttaa
    11221 gtttaattcc aactgcatta tcacaaggtt atgaagcgct ttatattggt tctaaaaatg
    11281 gtattgaaag agaaatgatt gaatcacaac taccagaaat taagtattat cctatttcga
    11341 gtggtaaatt aagaagatat atttctttag aaaatgccaa agacgtattt aaagtattga
    11401 aaggtattct tgatgctcgt aaagttttga aaaaagaaaa acctgatcta ttattttcaa
    11461 aaggtggatt tgtatctgtg cctgttgtta ttgcagccaa atcattaaat ataccaacta
    11521 ttattcatga atctgactta acaccaggat tagcgaataa gatagcactt aaatttgcca
    11581 agaaaatata tacaacattt gaagaaacgc taaactactt acctaaagag aaagctgatt
    11641 ttattggagc aacaattcga gaagatttaa aaaatggtaa tgcacataat ggttatcaat
    11701 taacaggctt taatgaaaat aaaaaagttt tacttgttat gggtggaagc ttaggaagta
    11761 aaaaattaaa tagcattatt cgcgaaaact tagatgcatt attacaacaa tatcaagtga
    11821 tacatttaac tggtaaagga ttaaaagatg ctcaagttaa aaaatcagga tatatacaat
    11881 atgaatttgt taaagaggat ttaacagatt tattagcaat tacggataca gtaataagta
    11941 gagctggatc aaatgcgatt tatgagttct taacattacg tataccaatg ttattagtac
    12001 cattaggttt agatcaatcc cgaggcgacc aaattgacaa tgcaaatcat tttgctgata
    12061 aaggttatgc taaaacgatt gatgaagaac aattaacagc acaaatttta ttacaagaac
    12121 taaatgaaat ggaacaggaa agaactcgaa ttatcaataa tatgaaatcg tatgaacaaa
    12181 gttatacgaa agaagcttta tttgataaga tgattaaaga cgcattgaat taatgggggg
    12241 taatgcttta tgagtcaatg gaaacgtatc tctttgctca tcgtttttgc attggttttt
    12301 ggaattatcg cgtttttcca cgaatcaaga ctagggaaat ggattgataa tgaagtttat
    12361 gaatttatat attcatcaga gagctttatt acgacatcta tcatgcttgg ggctactaaa
    12421 gtaggtgaag tctgggcaat gttatgtatt tcattacttc ttgtggcata tctcatgtta
    12481 aagcgccaca aaattgaagc attatttttt gcattaacaa tggcactatc tggaattttg
    12541 aatccagcat taaaaaatat attcgataga gaaagaccta cattgctgcg tttaattgat
    12601 ataacaggat ttagttttcc tagcggtcat gctatgggat caactgcata ttttggaagt
    12661 ggtatctatc tattaaatcg attaaatcaa ggtaattcaa aaggtattct tatagggtta
    12721 tgtgcagcta tgattttatt gatttccata tcacgtgtat atctaggtgt acattatcca
    12781 acagatatta ttgccggcat tattggtggc gtattttgca ttattttatc a
//
LOCUS       DANBWH010000065        12806 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.067, whole genome shotgun sequence.
ACCESSION   DANBWH010000065 DANBWH010000000
VERSION     DANBWH010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12806
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.067"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..384)
                     /locus_tag="PPY92_001264"
     CDS             complement(<1..384)
                     /locus_tag="PPY92_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HDJ2625276.1"
                     /translation="MNYLSNFFNTTIQLNIYIIVIVAACYIAIHQYRHKPVLNYLDVI
                     LNYIPVLTHEFGHVLFNKLAGGRAKDLVIVTSPRERQQTLQQGFAITQSRHLAGQWLT
                     TIGGYLMPPIMLLIGLASSHYQIPSF"
     gene            620..1384
                     /locus_tag="PPY92_001265"
     CDS             620..1384
                     /locus_tag="PPY92_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HDJ2625277.1"
                     /translation="MTVEERSNTAKVDILGVDFDNTTMLQMVENIKTFFANQSTNNLF
                     IVTANPEIVNYATTHQAYLELINQASYIVADGTGVVKASHRLKQPLAHRIPGIELMDE
                     CLKIAHVNHQKVFLLGATNEVVEAAQYALQQRYPNISFAHHHGYIDLEDETVVKRIKL
                     FKPDYIFVGMGFPKQEEWIMTHENQFESTVMMGVGGSLEVFAGAKKRAPYIFRKLNIE
                     WIYRALIDWKRIGRLKSIPIFMYKIAKAKRKIKKAK"
     gene            complement(1445..2239)
                     /gene="tagH"
                     /locus_tag="PPY92_001266"
     CDS             complement(1445..2239)
                     /gene="tagH"
                     /locus_tag="PPY92_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter
                     ATP-binding subunit TagH"
                     /protein_id="HDJ2625278.1"
                     /translation="MNVSVNIKNVTKEYRIYRTNKERMKDALIPKHKNKTFFALDDIS
                     LKAYEGDVIGLVGINGSGKSTLSNIIGGSLSPTVGKVDRNGEVSVIAISAGLSGQLTG
                     IENIEFKMLCMGFKRKEIKAMTPKIIEFSELGEFIYQPVKKYSSGMRAKLGFSINITV
                     NPDILVIDEALSVGDQTFAQKCLDKIYEFKEQNKTIFFVSHNLGQVRQFCTKIAWIEG
                     GKLKDYGELDDVLPKYEAFLNDFKKKSKAEQKEFRNKLDEARFVIK"
     gene            2586..3398
                     /gene="tagG"
                     /locus_tag="PPY92_001267"
     CDS             2586..3398
                     /gene="tagG"
                     /locus_tag="PPY92_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter permease
                     subunit TagG"
                     /protein_id="HDJ2625279.1"
                     /translation="MSAIGTVFKEHVKNFYLIQRLAQFQVKIINHSNYLGVAWELINP
                     VMQIMVYWMVFGLGIRSNAPIHGVPFVYWLLVGISMWFFINQGILEGTKAITQKFNQV
                     SKMNFPLSIIPTYIVTSRFYGHLGLLLLVIIACMFTGIYPSIHIIQLLIYVPFCFFLT
                     ASVTLLTSTLGVLVRDTQMLMQAILRILFYFSPILWLPKNHGISGLIHEMMKYNPVYF
                     IAESYRAAILYHEWYFMDHWKLMLYNFGIVAIFFAIGAYLHMKYRDQFADFL"
     gene            3497..4600
                     /locus_tag="PPY92_001268"
     CDS             3497..4600
                     /locus_tag="PPY92_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDJ2625280.1"
                     /translation="MNVLIKKFYHLVVRILSKMITPQVIDKPHIVFMMTFPEDIKPII
                     KALNNSSYQKTVLTTPKQAPYLSELSDDVDVIEMTNRTLVKQIKALKSAQMIIIDNYY
                     LLLGGYNKTSNQHIVQTWHASGALKNFGLTDHQVDVSDKAMVQQYRKVYQATDFYLVG
                     CEQMSQCFKQSLGATEEQMLYFGLPRINKYYTADRATVKAELKDKYGITNKLVLYVPT
                     YREDKADNRAIDKAYFEKCLPGYTLINKLHPSIEDSDIDDVSSIDTSTLMLMSDIIIS
                     DYSSLPIEASLLDIPTIFYVYDEGTYDKVRGLNQFYKAIPDSYKVYTEEDLIMTIQEK
                     EHLLSPLFKDWHKYNTDKSLHQLTEYIDKMVTK"
     gene            4597..5658
                     /locus_tag="PPY92_001269"
     CDS             4597..5658
                     /locus_tag="PPY92_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDJ2625281.1"
                     /translation="MRLTIIIPTCNNEATIRQLLISIESKEHYRILCIDGGSTDQTIP
                     MIERLQRELKHISLIQLQNASIATCINKGLMDIKMTDPHDSDAFMVINPTSIVLPGKL
                     DRLTAAFKNNDNIDMVIGQRAYNYHGEWKLKSADEFIKDNRIVTLTEQPDLLSMMSFD
                     GKLFSAKFAELQCDETLANTYNHAILVKAMQKATDIHLVSQMIVGDNDIDTHATSNDE
                     DFNRYIIEIMKIRQRVMEMLLLPEQRLLYSDMVDRILFNNSLKYYMNEHPAVTHTTIQ
                     LVKDYIMSMQHSDYVSQNMFDIINTVEFIGENWDREIYELWRQTLIQVGINRPTYKKF
                     LIQLKGRKFAHRTKSMLKR"
     gene            5721..6119
                     /gene="tagD"
                     /locus_tag="PPY92_001270"
     CDS             5721..6119
                     /gene="tagD"
                     /locus_tag="PPY92_001270"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HDJ2625282.1"
                     /translation="MKRVITYGTYDLLHYGHIELLRRAREMGDYLIVALSTDEFNQIK
                     HKKSYYDYEQRKMMLESIRYVDLVIPEKGWGQKEDDVEKFDVDVFVMGHDWEGEFDFL
                     KDKCEVIYLKRTEGISTTKIKQELYGKDAK"
     gene            complement(6236..7531)
                     /gene="pbp4"
                     /locus_tag="PPY92_001271"
     CDS             complement(6236..7531)
                     /gene="pbp4"
                     /locus_tag="PPY92_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP4"
                     /protein_id="HDJ2625283.1"
                     /translation="MKNLISIIIILCLTLSIMTPYAQATNSDVTPVQAANQYGYAGLS
                     AAYEPTSAVNVSQTGQLLYQYNIDTKWNPASMTKLMTMYLTLEAVNKGQLSLDDTVTM
                     TNKEYIMSTLPELSNTKLYPGQVWTIADLLQITVSNSSNAAALILAKKVSKNTSDFVD
                     LMNNKAKAIGMKNTHFVNPTGAENSRLRSFAPTKYKDQERTVTTARDYAILDLHVIKE
                     TPKILDFTKQLAPTTHAVTYYTFNFSLEGAKMSLPGTDGLKTGSSDTANYNHTITTKR
                     GKFRINQVIMGAGDYKNLGGEKQRSMMGNALMERSFDQYKYVKILSKGEQRINGKKYY
                     VENDLYDVLPSDFSKKDYKLVVEDGKVHADYPREFINKDYGPPTVEVHQPIIQKANTV
                     AKSMWEEHPLFTIIGGACLVAGLALIVHMIINRLFRKRK"
     gene            7952..9679
                     /locus_tag="PPY92_001272"
     CDS             7952..9679
                     /locus_tag="PPY92_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDJ2625284.1"
                     /translation="MKRENPLFFLFKKLSWPVGLIVAAITISSLGSLSGLLVPLFTGR
                     IVDKFSVSHINWNLIALFGGIFVINALLSGLGLYLLSKIGEKIIYAIRSVLWEHIIQL
                     KMPFFDKNESGQLMSRLTDDTKVINEFISQKLPNLLPSIVTLVGSLIMLFILDWKMTL
                     LTFITIPIFVLIMIPLGRIMQKISTSTQSEIANFSGLLGRVLTEMRLVKISNTERLEL
                     DNAHKNLNEIYKLGLKQAKIAAVVQPISGIVMLLTIAIILGFGALEIATGAITAGTLI
                     AMIFYVIQLSMPLINLSTLVTDYKKAVGASSRIYEIMQEPIEPTEALEDSENVLIDDG
                     VLSFEHVDFKYDVKKILDDVSFQIPQGQVSAFVGPSGSGKSTIFNLIERMYEIESGDI
                     KYGLESVYDIPLSKWRRKIGYVMQSNSMMSGTIRDNILYGINRHVSDEELINYAKLAN
                     CHDFIMQFDEGYDTLVGERGLKLSGGQRQRIDIARSFVKNPDILLLDEATANLDSESE
                     LKIQEALETLMEGRTTIVIAHRLSTIKKAGQIIFLDKGQVTGKGTHSELMASHAKYKN
                     FVVSQKLTD"
     gene            10036..11265
                     /locus_tag="PPY92_001273"
     CDS             10036..11265
                     /locus_tag="PPY92_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDJ2625285.1"
                     /translation="MFLLINIIGLIVFLGIAVLFSRDRKNIQWQSIGILVVLNLFLAW
                     FFIYFDWGQKAVRGAANGIAWVVQSAHAGTGFAFASLTNVKMMDMAVAALFPILLIVP
                     LFDILMYFNILPKIIGGIGWLLAKVTRQPKFESFFGIEMMFLGNTEALAVSSEQLKRM
                     NEMRVLTIAMMSMSSVSGAIVGAYVQMVPGELVLTAIPLNIVNAIIVSCLLNPVSVEE
                     KEDIIYSLKNNEVERQPFFSFLGDSVLAAGKLVLIIIAFVISFVALADLFDRFINLIT
                     GLIAGWIGIKGSFGLNQILGVFMYPFALLLGLPYDEAWLVAQQMAKKIVTNEFVVMGE
                     ISKDIASYTPHHRAVITTFLISFANFSTIGMIIGTLKGIVDKKTSDFVSKYVPMMLLS
                     GILVSLLTAAFVGLFAW"
     gene            11520..11612
                     /locus_tag="PPY92_001274"
     CDS             11520..11612
                     /locus_tag="PPY92_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625286.1"
                     /translation="MFCYYKQHKGDNFSIEEVKNIIADNEMKVN"
     gene            11790..12623
                     /locus_tag="PPY92_001275"
     CDS             11790..12623
                     /locus_tag="PPY92_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDJ2625287.1"
                     /translation="MNKTVKDLILVVLGSFIFAAGVNAFIISGNLGEGGVTGLAIILY
                     YAFHISPAITNFLVNAVLIAIGYKFLSKRSMYLTILVTILISIFLSLTESWQVETGNS
                     IVNAIFGGVSVGLGIGVIILAGGTTAGTTILARIATKYLDVSTPYALLFFDMIVVAIS
                     LTVIPLDKVLVTVISLYIGTKVMEYVIEGLNTKKAMTIISTNPDKLAKAIDEQIGRGL
                     TILNGHGYYTREEKDVLYVVISKTQVSKAKRLIKQIDKDAFLVIHDVRDVYGNGFLAD
                     E"
BASE COUNT         4400 a         1832 c         2259 g         4315 t
ORIGIN      
        1 gaaactaggt atctgataat ggctcgacgc taaacctatc aataacataa taggtggcat
       61 taaataacca ccaatcgttg taagccattg gcctgctaga tgtctagatt gtgtaattgc
      121 gaatccttgt tgtaatgtct gttgtcgctc tcgtggactt gttacaatga ctaaatcttt
      181 tgcacggcca ccagcgagtt tattaaacag tacatgacca aattcatgtg ttaaaacagg
      241 gatatagttt aaaatgacat ctaaatagtt caaaacaggc ttatgtctat attgatgaat
      301 agcaatataa caagctgcaa caataacgat aatgtatata ttaagttgaa ttgtcgtatt
      361 aaaaaagttt gataaataat tcattgttaa cctcatataa gatattaatt taaagtttgc
      421 ttagcactta ttataaatga tattggcatc aatagcgtta gactttagac ttaccttagt
      481 taaactaatt ttaatttttg aaaaggtgaa tatgtgttaa aataaagcaa aatcatttcg
      541 atataaatag gatgaatata aatactgtta atattgatta cactaacata ataatgaaat
      601 aagataggag attcctgtta tgactgttga agaaagatcc aatacagcca aagttgacat
      661 tttaggggtc gattttgata atacaacaat gttgcaaatg gttgaaaata ttaaaacctt
      721 ttttgcaaat caatcaacga ataatctttt tatagtaaca gccaaccctg aaatagtgaa
      781 ttacgcgacg acacatcaag cgtatttaga gttaataaat caagcgagct atattgttgc
      841 tgatgggaca ggagtagtca aagcttcgca tcgtttaaag caacctctag cgcatcgtat
      901 acctggtatt gagttgatgg atgaatgttt gaaaattgct catgtaaatc atcaaaaagt
      961 atttttgcta ggggcaacta atgaagttgt agaagcggca caatatgcat tgcaacaaag
     1021 atatccaaac atatcgtttg cacatcatca cggttatatt gatttagaag atgagacagt
     1081 agtaaaacga attaaactgt ttaaacctga ttacatattt gtaggtatgg gattccctaa
     1141 acaagaagaa tggattatga cacatgaaaa ccaatttgaa tctacagtga tgatgggcgt
     1201 aggtggttct cttgaagtat ttgctggggc taaaaagaga gcgccttata tatttagaaa
     1261 attaaacatt gaatggatat atagagcatt aatagattgg aaacgtattg gtagattgaa
     1321 gagtattcca atatttatgt ataaaatagc caaagcaaaa agaaaaataa aaaaagcgaa
     1381 atagtcatga tgataaaaat aaaaccgagg aaatccttaa atggagattc tcggtttttt
     1441 cggtttattt aataacgaag cgggcctcat cgagtttgtt tctaaattct ttttgttcgg
     1501 ctttggattt ctttttaaaa tcgttaagga aagcttcata tttaggtaat acatcatcaa
     1561 gttcaccgta atcttttaac tttccgcctt caatccaagc aatcttagta caaaattgtc
     1621 tcacttggcc taagttatga ctaacgaaaa agatggtttt gttttgctct ttaaactcgt
     1681 aaattttatc taaacatttt tgtgcaaaag tttggtcacc tacagataaa gcttcgtcaa
     1741 tgactaagat atctggatta actgtgatat taattgaaaa accaagtttt gcacgcatac
     1801 cacttgaata ctttttaact ggttgataaa taaactcacc aagttcacta aattcaataa
     1861 tcttaggtgt catcgcttta atttcttttc gcttaaagcc catacataac attttaaatt
     1921 cgatattttc aatccctgta agttgtccac tcaagccagc actaattgcg ataacgctga
     1981 cttcaccatt acgatccact ttgccaacag taggcgacaa agaaccgcca atgatattgc
     2041 tcaacgttga tttgccggaa ccattgatgc caacaagccc tatgacgtca ccttcatatg
     2101 cttttaaact aatgtcatct aaagcgaaaa atgttttgtt tttatgtttg ggaatgagcg
     2161 catctttcat acgttcttta tttgtacgat aaatacgata ttcttttgtt acatttttaa
     2221 tgtttaccga aacgttcatt tgtagacctt ccttattcac atttatctag attataatat
     2281 actactcaac agttgttaaa ttttaaaacc tgttgtaaag tgtatagaag attttgttat
     2341 tatcagagtt ggtgttttga cacaaaatgt taatcatcaa tgataacaat gatatttaaa
     2401 aactaaactt atttcaactt acatgattgt atactataat gtatttgtaa taaactaata
     2461 ttttaaagaa ctagacaata attttgatag catccatgta tagtgatagt atttacaaca
     2521 attattataa tactatttag ttaagtagag aaatagttaa acatttgaaa gtgtggttta
     2581 atggaatgtc agcaatagga acagttttta aagaacatgt aaagaacttt tatttaattc
     2641 aaagactggc tcagtttcaa gttaaaatta tcaatcatag taactattta ggtgtggctt
     2701 gggaattaat taaccctgtt atgcaaatta tggtttactg gatggttttt ggattaggaa
     2761 taagaagtaa tgcaccaatt catggtgtac cttttgttta ttggttattg gttggtatca
     2821 gtatgtggtt cttcatcaac caaggtattt tagaaggtac taaagcaatt acacaaaagt
     2881 ttaatcaagt atcgaaaatg aacttcccgt tatcgataat accgacatat attgtgacaa
     2941 gtagatttta tggacattta ggcttacttt tacttgtgat aattgcatgt atgtttactg
     3001 gtatttatcc atcaatacat atcattcaat tattgatata tgtaccgttt tgttttttct
     3061 taactgcctc ggtgacgtta ttaacatcaa cactcggtgt gttagttaga gatacacaaa
     3121 tgttaatgca agcaatatta agaatattat tttacttttc accaattttg tggctaccaa
     3181 agaaccatgg tatcagtggt ttaattcatg aaatgatgaa atataatcca gtttacttta
     3241 ttgctgaatc ataccgtgca gcaattttat atcacgaatg gtatttcatg gatcattgga
     3301 aattaatgtt atacaatttc ggtattgttg ccattttctt tgcaattggt gcgtacttac
     3361 acatgaaata tagagatcaa tttgcagact tcttgtaata tatttatatg acgaaacccc
     3421 gctaaccatt aataaatgga agtggggttc atttttgttt ataatttaag taaataacat
     3481 attaagttgg tgtattatga acgttttaat aaagaaattt tatcatttgg tagttcgaat
     3541 actttctaaa atgattacgc ctcaagtgat tgataaaccg catatcgtat ttatgatgac
     3601 ttttccagaa gatattaagc ctatcatcaa agcattaaat aattcgtcgt atcagaaaac
     3661 tgttttaaca acaccaaaac aagcgcctta tttatctgaa cttagcgacg atgttgatgt
     3721 gatagaaatg actaatcgaa cattggtaaa acaaattaag gctttgaaaa gcgcgcagat
     3781 gattattatc gataattatt acctattact aggtggatat aataagactt ctaatcaaca
     3841 cattgttcaa acgtggcatg caagtggtgc attaaaaaac tttggattaa cagatcatca
     3901 agtcgatgtg tctgacaagg caatggttca gcagtaccgt aaagtttatc aagcgacgga
     3961 tttttactta gtgggttgtg aacaaatgtc acaatgtttt aaacagtctt taggtgcaac
     4021 agaagagcaa atgctgtatt ttgggcttcc gagaattaat aaatattaca cagctgatag
     4081 agcaacggtt aaggcagagt taaaggataa atatggaatt acaaataagt tggtattata
     4141 tgtaccaaca tatagagaag ataaagcaga taatagggct attgataaag cttattttga
     4201 aaaatgttta ccaggatata cactgattaa taaattacat ccatcaattg aagattcaga
     4261 cattgatgac gtatcttcaa tcgacacgtc tacattaatg ctaatgtcag atataattat
     4321 tagcgactat agttcgctgc caatagaagc tagcttgtta gatattccaa ctatatttta
     4381 tgtgtatgat gaaggaacat atgataaagt gagaggcctg aatcaatttt acaaagcaat
     4441 accggatagc tacaaagtgt atactgaaga agatttaata atgacgatac aagaaaaaga
     4501 acatctatta agtccgttat ttaaagattg gcataagtat aatactgata aaagtttaca
     4561 tcagctcaca gaatatatag ataagatggt gacaaaatga ggcttacgat aatcatacct
     4621 acatgtaata atgaggcaac aattcgacaa ttgttaatat ctattgagag taaagaacac
     4681 tatagaatcc tttgtattga tggtggttct actgatcaaa caattcctat gattgaacgg
     4741 ttacaaagag aactcaagca tatttcatta atacaattac aaaatgcttc gatagctacg
     4801 tgtattaata aaggtttgat ggatatcaaa atgacagatc cacatgatag tgacgcattt
     4861 atggtcataa atccaacatc aatcgtattg ccaggtaaat tagataggtt aactgcagct
     4921 ttcaaaaata atgataatat tgatatggta atagggcagc gagcttacaa ttaccatggt
     4981 gaatggaaat tgaaaagtgc tgatgagttt attaaagata atcgaatcgt tacattaacg
     5041 gaacaaccag atttgttatc aatgatgtct tttgacggaa agttattcag tgctaaattt
     5101 gctgaattac agtgtgacga aactttagct aacacataca atcacgcaat acttgtcaag
     5161 gcgatgcaaa aagctacgga tatacattta gtttcacaga tgattgtcgg agataacgat
     5221 atagatacac atgctacaag taacgatgaa gattttaata gatatatcat agaaattatg
     5281 aaaataagac aacgagtcat ggaaatgtta ctattacctg aacaaaggct attatatagt
     5341 gatatggttg atcgtatttt attcaataat tcattaaaat attatatgaa tgaacaccca
     5401 gcagtaacgc acacgacaat tcaactcgta aaagactata ttatgtctat gcagcattct
     5461 gattatgtat cgcaaaacat gtttgacatt ataaatacag ttgaatttat tggtgagaat
     5521 tgggatagag aaatatacga attgtggcga caaacattaa ttcaagtggg cattaatagg
     5581 ccgacttata aaaaattctt gatacaactt aaagggagaa agtttgcaca tcgaacaaaa
     5641 tcaatgttaa aacgataacg tgtatattga tgaccataaa ctgcaatcct atgatgtgac
     5701 aatatgagga ggataactta atgaaacgtg taataacata tggcacatat gacttacttc
     5761 actatggtca tatcgaattg cttcgtcgtg caagagagat gggcgattat ttaatagtag
     5821 cattatcaac agatgaattt aatcaaatta aacataaaaa atcttattat gattatgaac
     5881 aacgaaaaat gatgcttgaa tcaatacgct atgtcgattt agtcattcca gaaaagggct
     5941 ggggacaaaa agaagacgat gtcgaaaaat ttgatgtaga tgtttttgtt atgggacatg
     6001 actgggaagg tgaattcgac ttcttaaagg ataaatgtga agtcatttat ttaaaacgta
     6061 cagaaggcat ttcgacgact aaaatcaaac aagaattata tggtaaagat gctaaataaa
     6121 ttatatagaa ctatcgatac taaacgataa attaacttag gttattataa aataaatata
     6181 aaacggacaa gtttcgcagc tttataatgt gcaacttgtc cgtttttagt atgttttatt
     6241 ttctttttct aaataaacga ttgattatca tatgaacaat aagtgctaat ccagcgacaa
     6301 ggcatgcacc accaatgata gtgaataatg gatgttcttc ccacatactt ttagcaacag
     6361 tatttgcctt ttgaataatt ggctgatgaa cttctacagt tggaggtcca taatctttat
     6421 taataaattc tcttggatag tccgcgtgta ctttaccatc ttcgactaca agtttataat
     6481 cttttttact aaaatcactt ggtaaaacat cgtaaagatc attttcaaca taatatttct
     6541 taccatttat cctttgctca cctttagaca atatttttac atatttatac tgatcaaatg
     6601 agcgttccat taatgcattc cccatcatac tacgttgctt ctcgccacca aggtttttat
     6661 agtctcctgc acccatgata acttgattaa ttctaaattt acctcgtttg gtagtaatcg
     6721 tatggttgta atttgctgta tcacttgatc cagtttttaa accatctgta ccaggcaaac
     6781 tcatttttgc accttccaat gaaaagttga atgtgtaata cgtaactgca tgcgttgttg
     6841 gtgctaactg ctttgtaaag tctaatattt taggtgtctc tttaatcacg tgtaaatcta
     6901 aaatggcata gtctctagca gtcgttacag tacgttcttg gtctttatac tttgttggtg
     6961 caaatgaacg taatcttgaa ttttcagcac ccgttggatt gacgaaatgt gtatttttca
     7021 ttccgatagc tttagctttg ttattcatta aatcaacgaa atcgctggtg ttttttgata
     7081 ccttcttagc taaaattaat gccgcggcat tactagaatt agatactgta atttgtaata
     7141 ggtctgcgat tgtccatact tgtccaggat atagtttcgt attactcaac tcaggtagtg
     7201 tagacataat atattctttg ttcgtcattg tgactgtgtc atcaagtgaa agctgcccct
     7261 tatttacagc ttccaatgtt aagtacattg tcattaattt agtcatagac gctggattcc
     7321 acttagtatc gatattgtat tgatacagta attgtccagt ttgacttaca ttaacagcac
     7381 tcgtcggttc gtatgcagcc gacaaacctg cataaccata ttgatttgct gcttgtacag
     7441 gggttacgtc actgttagta gcttgtgcat atggtgtcat aatacttaat gttaaacata
     7501 aaatgatgat aatagatatt aaatttttca taaagcgtta atcttccctt ttccaattct
     7561 taaatattcc ctaaaagcaa tggttattcc tacttacgga aatcattgct aattcacttc
     7621 accttaatta aattgttgaa aataaagttt tctgcagtta atttgaaaaa taatgcaaat
     7681 atattacgtg tgtagctaaa ggtgttataa tgtttgtacg aagagcaaac ttactcaaaa
     7741 gcgattaatt ttcatgtttt aatataaaga ctttgagaag ttattacaaa aaatgcaata
     7801 gaaatattct atcatataaa tgttatgagc ggtattttgg ggcaacactt tatttgattt
     7861 ttaaagtttt gttgggagaa agtatatgat agaaatgcat gtatctatct aaatgaatta
     7921 actataaatt tcaaacagaa gaggtaaaac tatgaaacga gaaaatccat tgtttttctt
     7981 atttaaaaaa ctatcatggc cagtgggtct tatcgttgca gctatcacta tttcatcact
     8041 agggagctta agtggactat tagtgccact gtttactgga cgaattgtag ataaattttc
     8101 cgtgagccat atcaattgga atctaatcgc attatttggt ggtatctttg tcatcaatgc
     8161 tttattaagc ggattaggtt tatatttatt aagtaaaatt ggtgaaaaga ttatttatgc
     8221 gatacgctca gttttatggg agcatatcat acaattaaaa atgccattct ttgacaaaaa
     8281 tgaaagtggt caattaatga gtcgattaac tgacgatacg aaagtgataa atgaatttat
     8341 ttcacaaaag ctacctaact tattaccatc aatcgttaca ttagttgggt cactaatcat
     8401 gttatttatt ttagattgga aaatgacatt attaacattt ataacgatac cgatattcgt
     8461 tttaattatg attcctctag gtcgtattat gcaaaagata tcgacaagta cacaatctga
     8521 aattgcaaac ttcagtggtt tgttagggcg tgtcctaact gaaatgcgtc ttgttaaaat
     8581 atcaaataca gagcgtcttg aattagataa tgcacataaa aatttgaatg aaatatataa
     8641 attaggttta aaacaggcta aaattgcggc agttgtacaa ccaatttcag gtatagttat
     8701 gttgctaaca attgcaatta ttttaggttt tggtgcatta gaaattgcga ctggtgcaat
     8761 cactgcaggt acattaattg caatgatatt ttatgttatt cagttatcta tgcctttaat
     8821 caatctttcc acgttagtta cagattataa aaaggcagtc ggtgcaagta gtagaatata
     8881 cgaaatcatg caagaaccta ttgaaccgac agaagctctt gaagattctg aaaatgtatt
     8941 aattgatgac ggtgtattgt catttgaaca tgtagacttt aaatatgatg tgaagaaaat
     9001 attagatgat gtgtcgttcc aaatcccaca aggtcaagtg agtgcttttg taggtccttc
     9061 tgggtctggt aaaagtacga tatttaatct gatagaacgt atgtatgaaa ttgagtcagg
     9121 tgatattaaa tatggccttg aaagtgtcta tgatatcccg ttatctaagt ggcgacgcaa
     9181 aattggatat gttatgcaat caaattcgat gatgagtgga acaattagag acaatatttt
     9241 atacggaatt aatcgtcatg tttcagatga agaacttatt aattatgcta aattagcgaa
     9301 ctgtcatgat tttatcatgc aatttgatga aggatatgac acgcttgtag gtgaacgagg
     9361 attgaaactg tctggcggac aacgtcaacg tattgatatt gctagaagtt ttgttaaaaa
     9421 tcctgatatt ttgttacttg atgaagcaac agctaatctc gatagtgaaa gtgaattgaa
     9481 aattcaagaa gctttagaaa cattgatgga aggtagaaca acgattgtca ttgcgcatcg
     9541 tttgtctaca attaaaaaag ccggtcaaat tatattctta gacaaaggac aggtaacagg
     9601 taaaggtacg cattcagaac tgatggcatc acatgcgaag tataaaaact ttgtagtgtc
     9661 tcaaaaatta acagattaat tttatatata agtaagcttg gagcaaatac acatatacca
     9721 tcgaggaaat taaagtgtgg cacattgatg gatatagatg ttaataaatt gcttcaagct
     9781 tttgtctatt ttaaatcatt tgagaagtta cgacataata attcttaaat taataaaatc
     9841 gatattttaa gaaaaaaatg ctcatggtat aatacaagtt ataagcaaac atacatatat
     9901 taaatactgt agccacgagt cataattctt catattttac atagcaattt aactgatttt
     9961 agagtccacg gtacagaagt ttgatatttc aatgtttcta aatttttaaa aaattaaatc
    10021 ataggtgggt gctaaatgtt tttattaatc aacattattg gtctaattgt atttcttggt
    10081 attgcggtat tattttcaag agatcgcaaa aatatccaat ggcaatcaat tgggatctta
    10141 gttgttttaa acctgttttt agcatggttc tttatttatt ttgattgggg tcaaaaagca
    10201 gtaagaggag cagccaatgg tatcgcttgg gtagttcagt cagcgcatgc tggtacaggt
    10261 tttgcatttg caagtttgac aaatgttaaa atgatggata tggctgttgc agccttattc
    10321 ccaatattat taatagtgcc attatttgat atcttaatgt actttaatat tttaccgaaa
    10381 attattggag gtattggttg gttactagct aaagtaacaa gacaacctaa attcgagtca
    10441 ttctttggga tagaaatgat gttcttagga aatactgaag cattagccgt atcaagtgag
    10501 caactaaaac gtatgaatga aatgcgtgta ttaacaatcg caatgatgtc aatgagctct
    10561 gtatccggag ctattgtagg tgcgtatgta caaatggtac caggagaact ggtactaacg
    10621 gcaattccac taaatatcgt taacgcgatt attgtgtcat gcttgttgaa tccagtaagt
    10681 gttgaagaga aagaagatat tatttacagt cttaaaaaca atgaagttga acgtcaacca
    10741 ttcttctcat tccttggaga ttctgtatta gcagcaggta aattagtatt aatcatcatc
    10801 gcatttgtta ttagttttgt agcgttagct gatctatttg atcgttttat caatttgatt
    10861 acaggattga tagcaggatg gataggcata aaaggtagtt tcggtttaaa ccaaatttta
    10921 ggtgtgttta tgtatccatt tgcgctatta ctcggtttac cttatgatga agcgtggttg
    10981 gtagcacaac aaatggctaa gaaaattgtt acaaatgaat ttgttgttat gggtgaaatt
    11041 tctaaagata ttgcatctta tacaccacac catcgtgcgg ttattacaac attcttaatt
    11101 tcatttgcaa acttctcaac gattggtatg attatcggta cattgaaagg cattgttgat
    11161 aaaaagacat cagactttgt atctaaatat gtacctatga tgctattatc aggtatccta
    11221 gtttcattat taacagcagc tttcgttggt ttatttgcat ggtaatatgt cgaagagtga
    11281 ctatgataat acattttaac taataaatat gtccaggcat gtcgtctatt gatataggtg
    11341 agatgcttgg acttttttat tattgatata aaggtattta aatattttta aagttaccga
    11401 aattgaagca ttataaaaac caagtgcaca tggtaataca cttggctttt atgggaaatg
    11461 aatattattg tacatatgac agtaaggact aggtacagtc atagtacttc gagcaaaatt
    11521 tgttttgtta ttataaacaa cacaaaggag ataacttctc tattgaagaa gttaaaaaca
    11581 ttatagcaga caatgaaatg aaagtaaatt aaaaattcag aatattttta attattatat
    11641 tgtgagtgat atttattagg gaaagctatt cttcatataa attagttaaa tagtaaatct
    11701 ttgttagaac tctttccata gagttgtgac agcatttttg ttgtgctact atttttatag
    11761 tctaataaat atataaaggg gatggtttcg tgaataaaac ggttaaagat ttaatactag
    11821 ttgtcttagg ttcatttatc tttgctgcag gtgtaaatgc atttattatt tctggtaact
    11881 taggtgaagg cggggttaca ggtttagcaa ttattttata ttatgcgttt catatttcac
    11941 cagccatcac taacttcttg gtcaacgcag tattgattgc cataggttat aaatttttga
    12001 gtaagagaag tatgtactta actattcttg taacaattct tatttcaata tttttgagtt
    12061 taacagaatc atggcaagta gaaactggaa acagcattgt gaatgccatt tttggtggtg
    12121 taagcgttgg actaggaatc ggagtaatta tccttgcagg cggtacaaca gcaggtacaa
    12181 caattttggc gagaattgca acgaaatacc tcgatgtaag cacgccatat gctttgcttt
    12241 tcttcgatat gatcgttgtt gcaatttcac ttacagttat tccacttgat aaagtattag
    12301 taacagtaat atcactttat ataggaacaa aagtgatgga atatgtcata gaaggtttaa
    12361 acactaaaaa agctatgacg attatttcaa ctaatcccga caaacttgcc aaagcaatag
    12421 acgagcaaat tggaagaggt ttaaccattt taaacggaca tggctattat acgcgtgaag
    12481 aaaaagatgt cttatacgtt gttatttcta aaacacaagt ttcaaaagca aagcgattaa
    12541 ttaaacaaat cgataaagat gcattcctcg taattcatga tgtaagagat gtctatggta
    12601 atggctttct tgcagatgaa taaataaatg gtatgagcac acatacttaa atagaagtcc
    12661 acggacaagt ttttgaacta tgaagactta tctgtgggcg ttttttattt tataaaagta
    12721 atatacaaga catgacaaat cgagctatcc aatttaaaaa gtaatgttag tcaataagat
    12781 tgaaaaatgt tataatgatg ttcatg
//
LOCUS       DANBWH010000066        12687 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.068, whole genome shotgun sequence.
ACCESSION   DANBWH010000066 DANBWH010000000
VERSION     DANBWH010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12687
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.068"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            66..1151
                     /locus_tag="PPY92_001276"
     CDS             66..1151
                     /locus_tag="PPY92_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDJ2625288.1"
                     /translation="MLNKVWFRTGIALIMLFILIKLFMEVHEVFTPIATIIGSVFLPF
                     LISGFLFYICLPFQNLLEKVGFPRWASITTIMLALFAIIGLIVAFVAPIIISNINNLI
                     SQTPALQKEAEQIIKFALAQMDKLPEDVTSRITNMVKSMGDGVTNILSNSLQYITSLI
                     STIFLLIMVPFFLIYMLKDHEKFIPAVAKFFKGERKVFFVDLLTDLNFTLKSYIQGQV
                     TVSVILGIFLYIGYSIIDLPYIPLLVLFAGVANLIPFLGSWLSFAPAAILGIIDSPTT
                     FIWVCIITLIAHQLEGNIITPNVMGKSLSIHPLTIIVVILAAGDLGGFTLVLIAVPLY
                     AVLKTVVSNIFKYRQRIIDKANSNVKD"
     gene            complement(1348..2118)
                     /gene="fabI"
                     /locus_tag="PPY92_001277"
     CDS             complement(1348..2118)
                     /gene="fabI"
                     /locus_tag="PPY92_001277"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDJ2625289.1"
                     /translation="MLNLENKTYVIMGIANKRSIAFGVAKVLDQLGAKLVFTYRKERS
                     RKELEKLLEQLNQPEAHLYQIDVQSDEEVINGFEQIGKDVGNIDGVYHSIAFANMEDL
                     RGRFSETSREGFLLAQDISSYSLTIVAHEAKKLMPEGGSIVATTYLGGEFAVQNYNVM
                     GVAKASLEANVKYLALDLGPDNIRVNAISAGPIRTLSAKGVGGFNTILKEIEERAPLK
                     RNVDQVEVGKTAAYLLSDLSSGVTGENIHVDSGFHAIK"
     gene            complement(2396..4240)
                     /locus_tag="PPY92_001278"
     CDS             complement(2396..4240)
                     /locus_tag="PPY92_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter family
                     protein"
                     /protein_id="HDJ2625290.1"
                     /translation="MEFLSLVIVVLAAFLTPIIVNRLNINFLPVVVAEILMGIVIGNS
                     FLNIVERDSILNILSTLGFIFLMFLSGLEIDFKAFKKDKRARQGQNDDESSIPGHLNL
                     ALTVFAFIMIISILLAYVFKWLGLVDDVLLMVIIISTISLGVVVPTLKEMNIMRTTIG
                     QFILLVAVLADLVTMILLTVYGAINGQGGSTIWLIGILVVFTAISYILGVQFKRMSFL
                     QKLMDGTTQIGIRAVFALIILLVALAEGVGAENILGAFLAGVVVSLLNPDEEMVEKLD
                     SFGYGFFIPIFFIMVGVDLNIPSLIKEPKLLIIIPILIVAFIISKLIPVMFIRRWFDM
                     KTTIASAFLLTSTLSLVIAAAKISERLNAISAETSGILILSAVITCVFVPIIFKKLFP
                     VPDEFNRKIEVSLIGKNQLTIPIAQNLTSQLYDVTLYYRKDLSDRRQLSDDITMIEIA
                     DYEQDVLERLGLFDRDIVVCATNDDDINRKVAKLAKAHQVERVICRLESTTDDTELVD
                     SGIEIFSSYLSNKILLKGLIETPNMLNLLSNVETSLYEIQMLNYKYENIQLRNFPFGG
                     DIIFVRIIRNNESIVPHGDTQLRYGDRLIVTGAKEYVDELKQELEFYF"
     gene            complement(4250..5635)
                     /gene="mgtE"
                     /locus_tag="PPY92_001279"
     CDS             complement(4250..5635)
                     /gene="mgtE"
                     /locus_tag="PPY92_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDJ2625291.1"
                     /translation="MSMNTDEKERVQEELYDETLLDQYLENDDIDQFRDEFLALHTYE
                     QSEYFEDTTDENRQKIFQYLSPEEVANFFDQLDIDDDEYELLFDKMNATYASHILEEM
                     SYDNAVDILNELTKPKVASLLTLMNKDDANEIKALLHYDEDTAGGIMTTEYLSLKAHT
                     PVKEALLLVKAQAPDAETIYVIFVVDDDGKLVGVLSLRDLIVAENDAYIEDIMNERVI
                     SVNVADDQEDVAQVMRDYDFMAVPVIDYQEHLLGIITIDDILDVMDEEASEDYSRLAG
                     VSDIDSTNDSIIKTALKRLPWLIILTFLGMITATILGRFEKTLENVALLAAFIPIISG
                     MSGNSGTQSLAVSVRNITTGEINEQSKFRIALREAGSGVLSGVVCSTILFTIIVAIYH
                     QPLLALIVAGSLTCAMTVGTFVGSMIPLLMNKLNIDPAVASGPFITTINDIISMLIYF
                     GLATSFMAYLI"
     gene            complement(5656..6510)
                     /locus_tag="PPY92_001280"
     CDS             complement(5656..6510)
                     /locus_tag="PPY92_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDJ2625292.1"
                     /translation="MKFKYHISQQETVKAFLARHDFSKKTVSAIKNNGALIVNDEPVT
                     VRKQLMPNDILEIHLPREIPSVNLIPYARKLEVLYEDAFIIIVTKPNNQNCTPSREHP
                     HESLIEQVLYHCQEHGENINPHIVTRLDRNTTGIVIFAKYGHIHHLFSKVNLKKIYTC
                     LVYGKTHTSGIIEANIRRSKDSIITREVASDGKYAKTSYEVINQNDKYSLCKVHLHTG
                     RTHQIRVHFQHIGHPIVGDSLYDGFHDKIHGQVLQCTQIYFVHPINKNNIYITIDYKQ
                     LLKLFNQL"
     gene            complement(6507..7316)
                     /locus_tag="PPY92_001281"
     CDS             complement(6507..7316)
                     /locus_tag="PPY92_001281"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HDJ2625293.1"
                     /translation="MRYTILTKGDSKSNALKHKMMNYMKDFRMIEDSENPEIVISVGG
                     DGTLLQAFHQYSHMLSKVAFVGVHTGHLGFYADWLPHEVEKLIIEINNSEFQVIEYPL
                     LEIIMRYNDNGYETRYLALNEATMKTENGSTLVVDVNLRGKHFERFRGDGLCVSTPSG
                     STAYNKALGGALIHPSLEAMQITEIASINNRVFRTVGSPLVLPKHHTCLISPVNHDTI
                     RMTIDHVSIKHKNVNSIQYRVANEKVRFARFRPFPFWKRVHDSFISSDEER"
     gene            complement(7333..7968)
                     /locus_tag="PPY92_001282"
     CDS             complement(7333..7968)
                     /locus_tag="PPY92_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDJ2625294.1"
                     /translation="MNQWDQFLTPYKQAVDELKVKLKGMRKQYEVGEQASPIEFVTGR
                     VKPIASIIDKANKRQIPFDRLREEMYDIAGLRMMCQFVEDIDVVVNILRQRKDFKVIE
                     ERDYIRNTKESGYRSYHVIIEYPIETLQGQKFILAEIQIRTLAMNFWATIEHTLRYKY
                     DGAYPDEIQHRLERAAEAAYLLDEEMSEIKDEIQEAQKYYTQKRSKKHEND"
     gene            complement(7985..8332)
                     /locus_tag="PPY92_001283"
     CDS             complement(7985..8332)
                     /locus_tag="PPY92_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625295.1"
                     /translation="MRLYINEIKIKDDILYCYTEDSIKGLSEVGQMLVDSDNYAFAYT
                     LDDGKAYAYLIFVQETWTMLHENTTKKIIINDELELTEFHQELTYILDNIKGNNNYGK
                     EFVATVEETFDIE"
     gene            8518..9111
                     /locus_tag="PPY92_001284"
     CDS             8518..9111
                     /locus_tag="PPY92_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDJ2625296.1"
                     /translation="MATNHEIEFKQMITASIYNKLQEKYFKDSVLFKQVNYYIDTPDF
                     KLKEHRSALRIRVKDNQYEMTLKTPAKVGLLEYNYIVDIKPEMNLIISNDNLPDDIRQ
                     IIVEQFGVKDTTLSILGALTTYRQETKYKGDLLVLDKSEYFDTADYELEFEVKDYNQG
                     LQKFQSLLNELNLEHHQPLNKVQRFFKKKETLSNNIN"
     gene            9215..9580
                     /locus_tag="PPY92_001285"
     CDS             9215..9580
                     /locus_tag="PPY92_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="truncated hemoglobin"
                     /protein_id="HDJ2625297.1"
                     /translation="MTTTPYDIIGKEALYDMIDYFYTLVEKDERLNHLFPGDFAETSR
                     KQKQFLTQFLGGPNIYTEEHGHPMLRKRHMDFTITEFERDAWLENMQTAINRAAFPQG
                     VGDYLFERLRLTANHMVNS"
     gene            9603..10409
                     /gene="yjbH"
                     /locus_tag="PPY92_001286"
     CDS             9603..10409
                     /gene="yjbH"
                     /locus_tag="PPY92_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease adaptor protein YjbH"
                     /protein_id="HDJ2625298.1"
                     /translation="MAGELRIMENKSREDINLSPVSKIEIYSFFDPFSSDCFKLSAIL
                     SKLRIEYNQYIRIRHILNPSLKVLTKCQAQSTSNFDNIALAYKAAELQGRVRAERFIH
                     LMQNEIIPKRDIITESMICDCIQNAGIDLEVFKDDLQKSKLTESLKIDLHIAREMEIE
                     QAPSLVFFSEDVHEEGLKVEGLYPYHIYTYIINELMGKPIEKNLPPKLETYIQQQQLV
                     TMEELLTIYEWPEKLLNKELKKLAIQQKIEKLKYPDGDFWKSKMPKIKSK"
     gene            complement(10869..12677)
                     /gene="pepF"
                     /locus_tag="PPY92_001287"
     CDS             complement(10869..12677)
                     /gene="pepF"
                     /locus_tag="PPY92_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDJ2625299.1"
                     /translation="MSQQLSREEQERKYPEYTWDLTTIFKDDEAFEAAFKEVENELGK
                     EEQFKGHIGDSAETLYNALELEDTLGTKLEKVYVYAHLKQDQDTTNDKYTGMESRAHQ
                     LIIKFSSAWSFLVPEILQIDEDKIQSFVNSYDKLQKFAFDLKLINEKRPHILDAETEK
                     LLTEAQDALSTPSNVYGMFSNADLVFEDAIDKDGNAHPLTQGTFIKYLESDDRKLRES
                     AFRNVYKAYGAHNNTLGATLAGEVKKNVFNARTHNYKTAREKALSNNHIPENVYDNLV
                     KTVHKYLPLLHRYTELRKELLGLDDLKMYDLYTPLIKDIKFEMPYEEAKEWMLKALEP
                     MGEEYLNVVKEGLNNRWVDVYENKGKRSGGYSSGAHLTNPFILLNWSNTISDLYTLVH
                     EFGHSAHSYFSRKFQPSNSSDYTIFVAEVASTCNEALLSDYMDKHLDDEKRLLLLNQE
                     LERFRATLFRQTMFAEFEHKIHAIEEAGEPLTPTRMNEEYAKLNKLYFGDSVETDEDI
                     SKEWSRIPHFYMNYYVYQYATGYSAAQSLSHQILTEGKPAVDRYINEFLKKGSSNYPI
                     EILKNAGVDMTTPEPIEQACEVFEQKLNAFEKLMKA"
BASE COUNT         4236 a         2240 c         1782 g         4429 t
ORIGIN      
        1 aagcactgct atttaggata tactaaataa taactaagtt tagaaaaata ggaggaacat
       61 cgtttatgtt aaacaaggtt tggttccgaa ctggcatcgc tctgattatg ctgttcattc
      121 tcatcaaact atttatggaa gtgcacgaag tatttactcc aatagctaca atcatcggtt
      181 ctgtatttct tccattttta attagtggtt ttttatttta tatctgtcta ccttttcaaa
      241 acttacttga aaaagtcggt tttccaagat gggctagtat aacaacaatc atgttagctt
      301 tgtttgctat tatcggctta attgttgcgt ttgttgcacc aatcatcatt tcaaatatca
      361 acaatttaat tagtcagaca cctgccctac aaaaagaagc agagcaaatt attaaattcg
      421 cactagctca aatggataaa ttacctgaag atgtaacaag tagaattaca aatatggtta
      481 aatcaatggg tgatggcgtt actaatattt tatctaattc attacagtac attacatcat
      541 taatttcaac catcttctta ttgattatgg ttccattctt cttaatttat atgttaaaag
      601 accatgaaaa atttatccca gctgttgcta aatttttcaa aggtgaacgt aaagtatttt
      661 tcgtagattt attaactgat ttaaacttta cattaaaatc ttacattcaa ggtcaagtaa
      721 ctgtcagtgt tattttaggt atattcttat atatcggtta ttcaattatt gacttaccat
      781 atattccttt attagttcta tttgctggtg tcgcaaactt aattcctttc ttaggttcat
      841 ggttatcatt tgcacctgca gcaatactcg gtattataga tagtcctact acatttatat
      901 gggtttgtat cattacatta attgcacacc aacttgaagg taatatcatt acgccaaatg
      961 taatgggtaa atctttaagt atccatcctt taacaattat cgttgttatt ttagcagcag
     1021 gagatttagg tggctttaca ttagttctga ttgcagtgcc attatatgct gtacttaaaa
     1081 cggttgttag taatattttc aaataccgcc aacgcattat tgataaagca aacagtaatg
     1141 ttaaggacta attctgtgga tgtcttttaa gaatataaga tactatctcc tcaaaagttg
     1201 aaactacagc ttttgaggag atttttttgt gcataaaaaa aatcagtcag atgaaatatc
     1261 aaataatttt ccatcagtcc gattattata aaagcaaaaa agctttgctc acatatatga
     1321 taacgtgaac aaagctgttg aatgatatta tttaattgcg tggaatccgc tatctacatg
     1381 aatattttca cctgtaacgc cacttgataa gtcacttaat aagtaagccg ctgttttacc
     1441 tacttctact tgatcaacgt tacgttttaa aggtgcacgc tcttcgattt ctttaagaat
     1501 tgtattgaaa ccacccacac cttttgcact taatgtacgg attggaccag ctgaaattgc
     1561 attaacgcga atattatcag gacctaagtc taatgctaaa tatttaacat ttgcttctaa
     1621 gctcgcttta gcaacaccca tcacattata attttgaact gcgaattcgc cacctaaata
     1681 tgttgttgca acaatgctac caccttctgg cattaatttt ttagcttcat gagccacaat
     1741 tgttaatgag taagaactaa tgtcttgagc taacaagaag ccttcacgtg aagtttcaga
     1801 aaagcgtccg cgtaagtctt ccatattagc aaatgcgatt gaatgatata caccatcaat
     1861 attgccaaca tctttaccaa tttgctcaaa accattaata acctcttcat cgctttgaac
     1921 atcaatttga tataagtgcg cttctggttg atttaattgt tctaataatt tttcaagctc
     1981 tttacggcta cgttctttac ggtaagtaaa tactaattta gcacctaatt gatctaaaac
     2041 tttagcgaca ccaaaagcaa tactacgctt attagcgatt cccatgatga catatgtttt
     2101 gttttcaaga tttaacatgt aagataactc cttataataa ttaatttaac ggatagaata
     2161 tctaatccca gttttaacta actttttatt cgtcaataca ttttaacact attatgactt
     2221 tttaataaag ttatttaaac gtattacaac gataaatgtt tgatttagtc ctttctgtat
     2281 caaacttgat ttacgtaaaa agcatttatt tatcaaaatc atatacatac tagcatttta
     2341 aaatcattaa ttttacgtcg tactaaaata cgtctgcatt ttaatatcat tattgttaaa
     2401 aataaaattc taactcttgc ttcaattcat caacgtattc tttagcaccg gtaacaatta
     2461 agcgatctcc atatcgcaat tgtgtatctc catgcggaac aatcgactca ttattacgga
     2521 taatacgcac gaagatgatg tctcctccga atgggaaatt acgtaattga atattttcat
     2581 atttataatt taacatttga atttcatata gtgacgtttc aacattactt aataaattca
     2641 acatgttagg tgtttcaatt aaacctttta ataagatttt attacttaag tagctactga
     2701 aaatttcaat acctgaatca actaactctg tatcgtccgt tgtgctttca agtctgcaaa
     2761 tgacacgctc aacttgatgt gctttggcta atttagcaac ttttcggtta atatcatcgt
     2821 cattcgtagc acaaacaact atgtctcggt caaacagacc tagtcgttct aaaacatctt
     2881 gttcataatc agcaatttct atcatcgtga tatcatctga caattgacga cgatcactca
     2941 agtctttgcg ataatataat gtcacgtcat ataactgaga tgttaaattt tgcgctatag
     3001 gaatcgttaa ttgattttta ccaattaaac taacttcaat tttacggtta aactcatctg
     3061 gaactggaaa cagttttttg aaaataatcg gaacgaatac acatgtaatg actgcgctta
     3121 aaattaatat acctgacgtt tcagctgaaa tagcatttaa tctttctgaa attttggctg
     3181 cagctatcac gagcgataat gttgatgtta ataaaaatgc tgatgcaatc gttgttttca
     3241 tatcaaacca acgtcgaata aacatgactg gaattaattt tgaaatgata aatgcaacga
     3301 ttaaaatcgg tatgataatt agtaatttcg gttctttaat taatgaaggt atgtttaaat
     3361 ctacaccaac cattataaag aaaataggaa taaagaaccc ataaccaaat gagtctaact
     3421 tttcaaccat ttcttcatct ggatttaata atgaaacaac gacacctgct aagaatgcac
     3481 ctaatatatt ttctgcgcca actccctctg ctagggctac taataatatt attaatgcaa
     3541 ataccgcacg aataccgatt tgcgtcgtac catccatcaa tttttgtaaa aatgacattc
     3601 ttttaaattg aacacctaaa atatatgaaa ttgctgtgaa aacaactaat atacctatta
     3661 accatattgt actgccgcct tgaccattga ttgcgccata gaccgttaat aaaatcatag
     3721 ttactaagtc cgcaagtact gctactaata ggataaattg ccctatagtt gttctcataa
     3781 tattcatttc ttttaaagtt ggaacaacta cgcctaagga aatagttgaa atgataatga
     3841 ccattaataa cacatcatcc actaatccaa gccatttaaa tacatacgct aaaagaatcg
     3901 aaataatcat aataaatgca aatacagtta acgctagatt aagatgccct ggaattgagg
     3961 attcatcatc attttgtcct tgacgtgcgc gtttatcttt tttaaaagct ttaaaatcaa
     4021 tttctaaacc acttaaaaac attaaaaaga taaagcctaa cgttgataaa atatttagaa
     4081 ttgaatccct ttctactata tttagaaatg aatttccaat cacaatcccc atcaaaattt
     4141 ctgcaacaac aactggcaag aaattaatat ttaatcgatt gacaattatt ggagttaaaa
     4201 acgctgctaa aacaacaata actaaagata aaaactccat aactcctcct taaattaagt
     4261 aagccataaa tgatgtagct aaaccaaaat aaatcaacat actaataata tcattaattg
     4321 ttgtaataaa tggtccacta gccactgctg gatcgatatt taatttattc atcaataatg
     4381 gaatcatcga acctacaaac gtccccaccg tcatcgcaca agttaaactt cctgcaacga
     4441 ttaatgctaa aagtggctga tgatatattg caacaataat tgtaaataat attgttgaac
     4501 atacaacgcc cgataataca ccacttcccg cttctcttaa tgcaattcta aatttacttt
     4561 gctcattaat ttcccctgtc gtaatgttac gaactgaaac ggctaaagat tgtgtacctg
     4621 aatttcctga cataccacta ataataggaa taaacgctgc gagtagcgct acattttcta
     4681 atgttttttc aaatctccct aaaattgtcg cagtaatcat tcctaaaaat gttaaaataa
     4741 tcaaccatgg taaacgtttt aatgctgttt taatgattga atcattagtc gaatcgatat
     4801 ctgatacccc ggctaaacga gagtagtctt cactagcctc ttcatccata acgtctaaaa
     4861 tatcatcaat cgtgatgata ccaagcaaat gttcttggta atctataaca ggtacagcca
     4921 tgaaatcata gtctctcata acttgagcaa catcttcttg gtcgtctgct acattcacac
     4981 taatgacacg ttcattcata atatcttcaa tataagcatc attttcagct acaattaaat
     5041 ctcttagtga taaaacacct actaatttac catcatcatc aacgacaaat ataacatata
     5101 ttgtttctgc gtctggcgct tgcgctttga ccaataataa cgcttcttta acaggcgtat
     5161 gcgctttaag tgataaatac tccgtcgtca taataccgcc ggccgtatcc tcatcatagt
     5221 gaagtaatgc tttgatttca ttcgcgtcat ctttattcat taatgttaaa agactagcaa
     5281 cttttggttt agtcaactca tttaaaatat ctactgcatt atcgtatgac atttcttcta
     5341 atatgtgact tgcgtatgtc gcattcattt tatcaaatag caactcatat tcatcgtcat
     5401 caatatctaa ttgatcaaag aaatttgcaa cctcttcagg tgataaatat tgaaaaatct
     5461 tttgtctatt ttcatcggta gtatcttcaa aatactcact ttgttcatat gtgtgtaatg
     5521 ctagaaattc atctctaaat tgatcaatat catcattttc taagtattga tctaataatg
     5581 tctcatcata taattcctct tgaacacgct ctttttcatc tgtgttcatt gacatactta
     5641 caccccctgt gtgaattaga gttgattgaa taatttaagt aattgcttat aatcaattgt
     5701 aatataaata ttgttcttat tgattggatg aacaaaatat atttgcgtac attgcagtac
     5761 ttgaccatga attttgtcat gaaaaccatc atacaaagaa tctcccacaa ttggatgccc
     5821 aatatgttga aaatgtacac gaatttgatg tgtacgtccc gtatgcaaat gaactttgca
     5881 taaactgtat ttatcattct gatttattac ttcataagat gttttagcgt atttaccatc
     5941 cgaggcaact tctctagtta taatactatc ctttgaccgt ctaatattag cttcaataat
     6001 accagatgta tgggttttgc catatacaag gcaagtatat atttttttca agtttacttt
     6061 agaaaataaa tgatggatat gtccatattt agcgaatatc acaataccag ttgtattacg
     6121 atctagacgc gtaacaatat gtgggttaat attttcacca tgttcctgac aatgatatag
     6181 tacttgttcg attaaacttt catgaggatg ttctctcgaa ggcgtacaat tttgattgtt
     6241 tggtttagta actatgatga taaaagcatc ttcatacaat acttctagct tacgagcata
     6301 aggtattaaa ttaacactcg gtatttctcg cggtaaatga atttctaata tatcatttgg
     6361 cattaattgc ttacgtactg tgactggttc atcattaaca attaaagcgc cattattttt
     6421 aatggcgctc actgtcttct tagaaaaatc atgtcgtgct aaaaaagctt taacagtttc
     6481 ttgttgtgat atatgatact taaatttcat cgttcttcat cacttgatat gaaagaatcg
     6541 tgtacacgtt tccagaacgg gaatggtcta aaacgtgcaa acctcacttt ttcatttgct
     6601 acacggtatt gtattgaatt aacattttta tgtttgatac taacatgatc tatcgtcatt
     6661 ctaatggtat catgattaac tggtgatatt aaacatgtat gatgctttgg taatacaagt
     6721 ggtgatccta ccgttctaaa cacacgatta tttattgagg caatttctgt aatttgcatt
     6781 gcttcaagtg aaggatgtat cagtgcgcca cctagcgctt tgttataagc cgttgaaccc
     6841 gaaggtgttg atacacataa tccatcgcct ctaaatcgct caaagtgttt ccctcttaag
     6901 ttaacatcca caacaagtgt tgagccattt tcagttttca tcgttgcttc atttaatgct
     6961 aaataccttg tttcatagcc gttgtcgttg tatctcataa taatttcaag caatggatat
     7021 tcaatgacct gaaactctga attattaatt tcgatgatta atttttcaac ttcatgaggt
     7081 aaccaatccg cataaaatcc taaatgacct gtatgaactc caacaaatgc cacttttgat
     7141 aacatgtggc tatactgatg gaatgcttgt agtaatgtac catcaccacc aactgaaata
     7201 acaatttcag gattttcact atcctcaatc atgcgaaaat ctttcatata gttcatcatt
     7261 ttatgcttta aggcattaga cttggagtca cctttagtta aaattgtata acgcatgatt
     7321 tataacacct cgttaatcat tttcatgttt tttagaacgt ttttgcgtgt aatatttttg
     7381 agcttcctga atttcatctt taatttcaga catctcttca tcaagtaaat acgctgcttc
     7441 tgccgctctt tccaaacgat gttgaatttc atccggataa gcaccatcat atttatatcg
     7501 taaagtatgt tcaatcgttg cccagaaatt cattgctaat gtacgaatct gaatctcagc
     7561 caatataaat ttttggcctt gtaatgtttc aattggatat tcaataatga catgatacga
     7621 gcggtaacca ctttctttag tgttacgaat ataatctcgt tcttcaatta ctttaaaatc
     7681 ttttctttgt cttaaaatat tgacgacaac atcaatatct tctacaaatt ggcacatcat
     7741 tcttaaacca gcgatatcgt acatttcttc tcttaaccta tcaaatggta tttgtcgttt
     7801 gtttgcctta tctataatac tagcgatcgg tttaacacga ccagtaacaa attctattgg
     7861 cgacgcttgt tcaccaactt catattgttt gcgcatgcct ttaagtttca ctttcaactc
     7921 atcaaccgct tgcttataag gtgttaagaa ctgatcccat tgattcatag tgcttcaccc
     7981 cgctttattc aatgtcgaat gtttcttcaa cggttgcaac aaattcctta ccataattat
     8041 tattcccttt tatgttgtct aaaatataag taagttcttg gtggaattca gtcaattcta
     8101 gttcatcatt gataataatt tttttagtcg tgttttcatg caacatcgtc catgtttctt
     8161 gtacgaaaat gagataagca tacgctttac catcatctaa tgtatacgca aaggcataat
     8221 tatcactatc aacgagcatt tgtcctactt cagataatcc tttaatagaa tcttctgtat
     8281 aacaataaag tatgtcatct ttaattttaa tttcattaat atataaacgc atgcttaatc
     8341 ctcctcttat tccataacat tttaacacaa ttcaataata caaaacactt acgaacacta
     8401 tttttaatgg tcaaattaac attgataata gataaaggaa attgctttta aaattcaact
     8461 ttgtcattca taatatagtt aagataaatt gaatttgata cgaggtgtat acatatcatg
     8521 gcaacaaatc atgaaataga atttaaacaa atgataactg catcaatcta taataaattg
     8581 caagagaaat attttaaaga cagtgtgctt tttaagcaag tcaattacta tattgataca
     8641 cccgatttca aattaaagga acatcgctct gcactacgca taagagttaa agacaatcaa
     8701 tatgaaatga ctttaaagac accagcaaag gttggtctat tagaatacaa ctatatcgtc
     8761 gacattaaac cagagatgaa cttgattatt tcaaacgaca atcttccaga tgacattcgt
     8821 caaattatag ttgaacagtt tggtgtaaaa gatacaactt tatcaatact tggtgcatta
     8881 acgacatatc gccaagaaac taaatataaa ggtgatttac tcgttttaga taaaagtgag
     8941 tatttcgata ctgcggatta tgagttagag ttcgaagtta aagactataa tcaaggttta
     9001 caaaagttcc aatctttatt aaacgaacta aacctcgaac atcatcaacc tttaaataaa
     9061 gttcaacgtt ttttcaagaa aaaagaaaca ctttcaaaca atataaatta aattggcaaa
     9121 tatgcatatt tcttccaaat gtttaaaatc atgttatatt agttatatat taacttaagt
     9181 tcgtaatagt aaatttttga aaatgggtga ttaaatgaca acaacaccat atgacatcat
     9241 tggtaaagaa gcgttatacg atatgattga ttatttttac acccttgtag aaaaagatga
     9301 acgacttaat cacctgtttc caggagattt tgcagaaaca agtcgtaaac aaaaacaatt
     9361 tttaacacag tttttgggcg gtccaaacat ttataccgaa gaacacggac atcctatgct
     9421 aagaaaaaga catatggatt ttacaatcac tgaatttgaa agagatgcat ggttagaaaa
     9481 tatgcagaca gcaattaatc gcgctgcgtt tccacaaggt gttggagatt atttatttga
     9541 gcgcttaaga ttaactgcta atcatatggt gaattcctaa aattaattgt aggtgaataa
     9601 acatggctgg agaattacga ataatggaaa ataagagtcg tgaagatatt aatctatcac
     9661 ctgtaagtaa aatcgaaatt tattcatttt tcgatccatt tagctccgat tgcttcaaat
     9721 tatcagcaat cttatccaaa ttaagaattg aatataatca atatatacgt atcagacata
     9781 tattaaatcc ttcgttaaaa gtattaacga aatgccaagc tcaaagtaca tccaactttg
     9841 ataacatcgc cctagcttat aaagcagctg agttacaagg tcgtgtacgt gccgaacgat
     9901 ttatacattt aatgcaaaat gaaatcatac ctaaaagaga tattattaca gaatcaatga
     9961 tttgtgactg tattcaaaat gcaggtattg atttagaagt atttaaagac gacttacaaa
    10021 aaagtaaact aaccgaaagc ttgaaaattg atttgcatat tgcaagagaa atggaaatcg
    10081 aacaagcccc ttctctcgtt ttctttagtg aagatgttca tgaagaaggt ttaaaagtcg
    10141 aaggattata cccatatcac atctatactt atataattaa tgaattgatg ggtaaaccta
    10201 tcgaaaagaa tcttcctcct aaattagaaa cttatataca gcaacaacaa cttgtaacga
    10261 tggaagaatt acttactatt tatgaatggc cagaaaaact tttaaacaaa gagttaaaga
    10321 agttagccat tcaacaaaaa attgaaaaac ttaaatatcc agacggcgat ttctggaaat
    10381 ctaaaatgcc taaaatcaaa tcaaaataaa taaattaaag acctcgtaca ccataaatgt
    10441 aacgaggtct ttttactgac tcttaatttt gcaacattaa attattagaa cgtaatgtta
    10501 cacatcgatc taaatatatt agtgatattt ttcgaattta accagttcga aaattataca
    10561 aattataaat ttggtacata atagacttat attaatcgtt aatgtataaa aagtgcatat
    10621 ttaatacatt tttatacaac ttcacttctc tcttattaaa aaagaacata aaaaaacgtc
    10681 gtgtctgaat acacgacgct aaacaaaggg gatgggagaa atttttcact tcaaacaaag
    10741 gggtatgttt gttatgtgat taatttcatg ttcataatat aacatgctat ctctaccatt
    10801 tcaacctttt aatagtactt aatttaaatt gtcacaatct gttcataatg taagcgaata
    10861 caatagtact aagctttcat taatttttca aaagcgttca atttttgttc aaaaacttca
    10921 caagcttgtt caattggttc aggtgttgtc atatctacac cagcattttt taatatctca
    10981 attggataat ttgagctacc ttttttcaag aattcattaa tatatctatc tactgctggc
    11041 ttaccttctg ttaaaatttg atgacttaag ctttgagctg cactgtaacc agttgcgtat
    11101 tgatatacat aataattcat atagaagtgt ggaatacgtg accattcctt actaatatct
    11161 tcatcagttt ctacagaatc accgaagtat aatttattta atttggcata ttcttcattc
    11221 attctagttg gcgttaatgg ttcacctgct tcttcaattg catgaatttt atgctcaaat
    11281 tctgcgaaca ttgtttgtcg gaataatgta gctctgaaac gttctaattc ttggtttaat
    11341 aataataagc gtttttcatc atcaagatgt ttatccatat aatcacttaa aagtgcttcg
    11401 ttacaagttg atgcaacttc agcgacaaaa atagtgtagt cactagaatt tgacggttgg
    11461 aattttctac tgaagtaact atgtgctgaa tgcccaaatt catgaactaa tgtgtataag
    11521 tctgaaatag tattagacca gttaagtaga ataaatggat tagttaaatg tgcacctgat
    11581 gaatagccac ctgaacgttt acctttattc tcatagacat cgacccaacg attgtttaag
    11641 ccttctttaa ctacatttaa atattcttca cccattggtt ctaaagcttt taacatccac
    11701 tctttagctt cttcataagg catttcaaac ttaatatctt taattaatgg tgtatataaa
    11761 tcatacattt tcaagtcatc taaacctagc aattctttgc gcaattcagt atatctatgt
    11821 agcaatggta aatatttatg tacagttttt actagattgt catatacatt ttctggaata
    11881 tgattattac ttaatgcttt ttctcttgca gttttgtaat tgtgtgtacg agcattaaat
    11941 acatttttct tcacttcacc tgctagcgta gcgccaagcg tattattatg agcaccatat
    12001 gctttatata catttctaaa agcactttct cttagtttgc gatcatctga ttctaaatac
    12061 ttaataaatg taccttgtgt taacgggtgt gcatttccat ctttatcaat cgcatcttca
    12121 aatactaaat cagcgttgct aaacataccg tatacatttg atggcgttga taacgcgtcc
    12181 tgcgcttctg ttaataactt ttcagtttca gcatctaaaa tatgaggacg tttttcatta
    12241 atcaacttca aatcaaatgc gaatttttgt aatttatcat atgaatttac aaatgattga
    12301 attttatctt catcaatttg taaaatctct ggcactaaga aactccatgc cgagctaaat
    12361 ttaataatta attgatgtgc tcttgactcc ataccagtat acttgtcgtt cgttgtgtct
    12421 tggtcttgtt ttaggtgcgc gtatacatat actttttcta atttagtacc taatgtatct
    12481 tctaattcta acgcattgta taatgtctca gcactatcac caatgtgtcc tttaaattgt
    12541 tcttctttgc ctaactcatt ttcaacttct ttaaatgcag cctcaaaagc ttcatcatct
    12601 ttgaaaattg ttgttaagtc ccatgtatat tcaggatatt tacgttcctg ttcttctctc
    12661 gataattgtt gactcatatg tagtaaa
//
LOCUS       DANBWH010000067        12589 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.069, whole genome shotgun sequence.
ACCESSION   DANBWH010000067 DANBWH010000000
VERSION     DANBWH010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12589
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.069"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..251)
                     /locus_tag="PPY92_001288"
     CDS             complement(<1..251)
                     /locus_tag="PPY92_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDJ2625300.1"
                     /translation="MANIKSAIKRVKTTEKAEARNISQKSAMRTAVKNAKTAVSNNAD
                     NKNELVSLAVKLVDKAAQSNLIHSNKADRIKSQLMTANK"
     gene            597..2420
                     /gene="lepA"
                     /locus_tag="PPY92_001289"
     CDS             597..2420
                     /gene="lepA"
                     /locus_tag="PPY92_001289"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDJ2625301.1"
                     /translation="MDNEQRLKRRENIRNFSIIAHIDHGKSTLADRILENTKSVETRD
                     MQDQLLDSMDLERERGITIKLNAVRLKYEAKDGNTYTFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNELELLPVINKIDLPAAEPERVKQEIEDMI
                     GLDQDDVVLASAKSNIGIEEILEKIVEVVPAPDGDPEAPLKALIFDSEYDPYRGVISS
                     IRIVDGVVKAGDKIRMMATGKEFEVTEVGINTPKQLPVDELTVGDVGYIIASIKNVDD
                     SRVGDTITLASRPASEPLQGYKKMNPMVYCGLFPIDNKNYNDLREALEKLQLNDASLE
                     FEPESSQALGFGYRTGFLGMLHMEIIQERIEREFGIELIATAPSVIYQCILRDGSEVT
                     VDNPAQMPDRDKIDKIFEPYVRATMMVPNDYVGAVMELCQRKRGQFINMDYLDDIRVN
                     IVYELPLAEVVFDFFDQLKSNTKGYASFDYEFIENKESNLVKMDILLNGDKVDALSFI
                     VHRDFAYERGKALVEKLKTLIPRQQFEVPVQAAIGQKIVARTNIKSMGKNVLAKCYGG
                     DISRKRKLLEKQKAGKAKMKAVGNVEIPQDAFLAVLKMDDE"
     gene            2986..4110
                     /locus_tag="PPY92_001290"
     CDS             2986..4110
                     /locus_tag="PPY92_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDJ2625302.1"
                     /translation="MTVQSAYIHIPFCVRICTYCDFNKYFIQNQPVDEYLDALITEMS
                     TAKYRNLKTMYVGGGTPTALSINQLERLLKAIRDTFTITGEYTFEANPDELTKEKVQL
                     LEKYGVNRISMGVQTFKPELLSVLGRTHNTEDIYTSVLNAKNAGIKSISLDLMYHLPK
                     QTIEDFEQSLDLALDMDIQHISSYGLILEPKTQFYNMYRKGLLKLPNEDLGADMYQLL
                     MSKIEQSPFHQYEISNFALDGHESEHNKVYWFNEEYYGFGAGASGYVDGVRYTNINPV
                     NHYIKAINKESKAILVSNKPSLTERMEEEMFLGLRLNEGVSSSRFKKKFDQSIESVFG
                     QTINNLKEKELIVEKNDAIALTKRGKVIGNEVFEAFLIND"
     gene            4211..5188
                     /gene="hrcA"
                     /locus_tag="PPY92_001291"
     CDS             4211..5188
                     /gene="hrcA"
                     /locus_tag="PPY92_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HDJ2625303.1"
                     /translation="MITDRQLSILNAIVEDYVDFGQPVGSKTLIERHNLNVSPATIRN
                     EMKQLEDLNYIEKTHSSSGRSPSQLGFRYYVNRLLEQTSHQKTNKLRRLNQLLVENQY
                     DVSSALTYFADELSNISQYTTLVVHPNHKQDIINNVHLIRANPNLVIMVIVFSSGHVE
                     HVHLASDIPFSNDKLNTISNFVTNKLTEFNQNLQDDIVSFVQSEQEEIFINKLINTMN
                     NHISNQSNSIYMGGKVKLIDALNESNVSSIQPILQYIESNRIAELLQDISSPNINVKI
                     GNEIDDSLSDISIVTSQYHFDETLKGQIAVIGPTAMHYQNVIQLLNRIW"
     gene            5220..5846
                     /gene="grpE"
                     /locus_tag="PPY92_001292"
     CDS             5220..5846
                     /gene="grpE"
                     /locus_tag="PPY92_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDJ2625304.1"
                     /translation="MTNKDESVEKNTESTVEETNIKQNIDDSVEQAEESKGHLQDEAI
                     EETSDENVIEEIDPKDQKINELQQLADENEEKYLRLYAEFENYKRRIQKENEINKTYQ
                     AQRVLTDILPAIDNIERALQIEGDDETFKSLQKGVQMVHESLINALKDNGLEVIKTEG
                     EAFDPNIHQAVVQDDNPDFESGEITQELQKGYKLKDRVLRPSMVKVNQ"
     gene            5915..7747
                     /gene="dnaK"
                     /locus_tag="PPY92_001293"
     CDS             5915..7747
                     /gene="dnaK"
                     /locus_tag="PPY92_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDJ2625305.1"
                     /translation="MSKIIGIDLGTTNSCVTVLEGDEPKVIQNPEGSRTTPSVVAFKN
                     GETQVGEVAKRQAITNPNTVQSIKRHMGTDYKVDIEGKSYTPQEISAMILQNLKNTAE
                     SYLGEKVDKAVITVPAYFNDAERQATKDAGKIAGLEVERIINEPTAAALAYGLDKTDK
                     DEKVLVFDLGGGTFDVSILELGDGVFEVLSTAGDNKLGGDDFDQVIIDYLVAEFKKEN
                     GVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFE
                     ELSDSLIRRTMEPTRQAMKDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEIGKEPNKG
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSK
                     SQIYSTAVDNQPSVDVHVLQGERPMAADNKTLGRFQLTDIPPAERGKPQIEVTFDIDK
                     NGIVNVTAKDLGTNKEQRITIQSSSSLSDEEIDRMVKDAEVNAEADKKRREEVDLRNE
                     ADSLVFQVEKTLTDLGENIGEEDKKSAEEKKDALKTALEGQDIEDIKSKKEELEKVIQ
                     ELSAKVYEQAAQQQQQAQGANAGQNNDSTVEDAEFKEVKDDDKK"
     gene            7883..9022
                     /gene="dnaJ"
                     /locus_tag="PPY92_001294"
     CDS             7883..9022
                     /gene="dnaJ"
                     /locus_tag="PPY92_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDJ2625306.1"
                     /translation="MAKRDYYEVLGISKDASKDEIKKAYRKLSKKYHPDINKEEGADE
                     KFKEISEAYEVLSDDNKRASYDQFGHDGPQGFGGQGFNGSDFGGFSGFGGGGFEDIFS
                     SFFGGGRQRDPNAPQKGDDLQYTMTLTFEEAVFGTTKEISIRKDVTCETCHGDGAKPG
                     TSKKTCSYCNGAGHVAVEQNTILGRVRTEQVCPKCNGSGQEFEEACPTCHGKGTENKT
                     VKLEVKVPEGVDNEQQIRLAGEGSPGVNGGPAGDLYVVFRVKPSETFKRDGDDIYYKL
                     NVSFPQAALGDEIKIPTLNNEVMLTIPAGTQTGKQFRLKEKGIKNVHGYGYGDLYVDI
                     KVVTPTKLTDRQKELMKEFAQLNGEEINEQPSNFKDRAKRFFKGE"
     gene            9026..9964
                     /gene="prmA"
                     /locus_tag="PPY92_001295"
     CDS             9026..9964
                     /gene="prmA"
                     /locus_tag="PPY92_001295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDJ2625307.1"
                     /translation="MNWTELSIIINHEAVELATNILENHGSNGVVIEDSDDLINQPED
                     KYGEIYALKKEDYPDKGVRLKAYFNEMTYDDKLRQQIKDELLNLDELDQHNIQFSEQI
                     IAETDWENEWKNYFHPFRASKKFTIVPSWETYAKEADEELCIELDPGMAFGTGDHPTT
                     SMCLKAIETYVLPQHSVIDVGTGSGILSIASHLIGVKRIKALDIDEMAVSVAKENFRR
                     NHCETLIEAVPGNLLKDETEKFDIVIANILAHIIDEMIEDAYNTLNEGGYFITSGIIK
                     EKYEGIQSHMERVGFKIISEQHDNGWVCLVGQKVSE"
     gene            9966..10718
                     /locus_tag="PPY92_001296"
     CDS             9966..10718
                     /locus_tag="PPY92_001296"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDJ2625308.1"
                     /translation="MQRYFIDQNADVSQRFFITKKEDIHHITNVMRYDVGSKIILTFN
                     DQTVYLCEIADITSDKIAVDLKEKQNINTELPVDVTICSGLIKADKYEWLLQKATELG
                     ASSFIAVSMERSIVKLNEAKVVKKIERWQKIIKEAAEQSYRLVIPSIQFESNLKLICD
                     TIDNYDYILIAYEEEAKDGELSNFKQTLQQFKAQDKVLMIFGPEGGLSENEISLFSNT
                     STVVGLGPRILRAETAPLYALSAISYEKELMG"
     gene            10725..12071
                     /gene="mtaB"
                     /locus_tag="PPY92_001297"
     CDS             10725..12071
                     /gene="mtaB"
                     /locus_tag="PPY92_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HDJ2625309.1"
                     /translation="MSTVAFHTLGCKVNHYETEAIWQLFKEANYERVDFEANADVFVI
                     NTCTVTNTGDKKSRQIIRRAIRQNPDAVICVTGCYAQTSSAEIMEIPGVDVVVGTQDR
                     HKLLGYIDEFRKERQPINGVGNIMKNRKYEELDVPYFTDRTRASLKIQEGCNNFCTFC
                     IIPWARGLMRSRDPEKVVEQATQLVNSGYKEIVLTGIHTGGYGQDLKDYNLAQLLRDL
                     ETINGLERIRISSIEASQLTDEVIDVLERSTKVVRHLHIPLQSGSDTVLKRMRRKYTM
                     DRFSERLTKLHKALPDLAVTSDVIVGFPGETEAEFQETYDFIVKHKFSELHVFPYSPR
                     IGTPAARMDDQIDEEIKNERVHKLITLSNQLGKLYVSKFDQDVLEVIPEEQGDTEGTL
                     VGYADNYMKVQFEGDESLIGQIVKVKITQANYPLNEGQAIKVVDFATNKSDREVLV"
     gene            12364..12540
                     /locus_tag="PPY92_001298"
     CDS             12364..12540
                     /locus_tag="PPY92_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDJ2625310.1"
                     /translation="MSKTVVRKNESLEDALRRFKRSVSKSGTIQEVRKREFYEKPSVK
                     RKKKSEAARKRKFK"
BASE COUNT         4720 a         1723 c         2418 g         3728 t
ORIGIN      
        1 tatttatttg cagtcattaa ttgtgattta atacggtcag ctttgtttga atgtattaaa
       61 ttactttgag cagctttgtc tactaactta actgctaagc ttactaattc atttttatta
      121 tcagcgttat ttgaaacagc tgttttagcg tttttaactg ctgtacgcat tgcactcttt
      181 tgtgaaatgt tgcgtgcttc agctttttca gttgttttta cacgtttaat tgcagatttg
      241 atatttgcca tttctgtcac ctcctaaaag tgagttcatc ttatcaaaat tttatttgat
      301 tacaacaaga aatattttaa caaaagtcta accgttgtgc aatcatttat ttgatggggt
      361 tagcttaaaa ttttgcagat ttaataaatt gtgttggaaa cacaatcaat acgttttata
      421 attataagta cgtaattaac atattaacat atcgcaagta tgtatttaaa taaagattgt
      481 tataatttca aagttcatcc aagattatgg cgtttgcatt tacctattaa aaacgttatt
      541 atatcaaaga tgcgaaagat aatacgggtt tattttatga aagtgagaag gataaaatgg
      601 ataatgagca acgcttaaaa agaagagaga atataaggaa tttctcgatt atagcacata
      661 ttgaccacgg aaaatctaca ttggctgata gaattttaga aaataccaaa tcagttgaaa
      721 caagagatat gcaagatcag ttactagatt caatggattt agaaagagaa cgtggtatta
      781 caatcaaatt aaacgcagtt cgtttaaagt acgaagctaa agatggaaat acttatacat
      841 tccatttaat cgatacgcct ggacacgtcg attttacata tgaagtgtca cgttctttgg
      901 cagcttgtga gggtgcgatt ttagtagtag atgcggctca aggtatcgaa gcacaaacat
      961 tagcaaatgt ttatttggca ttagataatg agttagagtt attgcctgtt attaacaaaa
     1021 ttgatttacc tgctgcagaa cctgaacgcg tgaaacaaga aattgaagat atgataggtt
     1081 tagaccaaga cgatgttgtt ttagcaagtg ctaaatctaa cattggaatt gaagagatac
     1141 tagagaaaat agttgaagtt gtgccagctc cagatggtga cccagaagca ccactaaaag
     1201 cattaatatt tgattctgag tatgatccat atagaggggt aatttcatcg ataagaattg
     1261 tagacggtgt tgttaaagct ggagataaaa ttcgaatgat ggccactggt aaagagttcg
     1321 aagtaacaga agttggaatt aatacaccta agcagcttcc agttgatgaa ttaacagttg
     1381 gtgatgttgg ttatattatt gcaagtatta aaaatgttga tgattctagg gttggtgaca
     1441 ccatcacatt agctagtaga cctgcatcag aaccattgca aggttataag aaaatgaatc
     1501 caatggtata ttgcggactg ttcccaatag ataacaaaaa ttataatgat ttaagagaag
     1561 cattagaaaa attacaattg aatgatgcat cattagaatt tgagcctgaa tcgtcacaag
     1621 cattaggttt tggttataga actggtttct taggtatgtt acacatggaa ataattcaag
     1681 aaagaattga aagagaattt ggcattgaat taattgcaac agcaccatct gtaatttatc
     1741 aatgtatttt aagggacggt tcagaagtga cggttgataa cccagcacaa atgccagatc
     1801 gtgataaaat tgataaaata tttgagccat atgttcgtgc aactatgatg gttccaaatg
     1861 actatgtagg tgcagtaatg gaattatgtc aacgtaaacg tggacaattt ataaatatgg
     1921 actatttaga tgatattcgt gtaaatattg tttatgaatt acctttagct gaagttgtat
     1981 ttgatttctt cgatcaactt aaatctaata ctaaaggata tgcatcattt gattatgaat
     2041 tcatcgaaaa taaagaaagt aatttagtta agatggatat tttattaaac ggtgataaag
     2101 tggatgcgct gagcttcata gttcatagag attttgcata tgaacgtggt aaagcattag
     2161 ttgaaaaact taaaacgtta attccaagac agcaatttga agtacctgta caggctgcaa
     2221 taggacaaaa aattgtagcg cgtacaaata ttaaatcaat gggtaaaaac gttttagcta
     2281 aatgttatgg cggtgacata agccgtaaac gtaaattact tgaaaaacaa aaagcaggta
     2341 aagctaagat gaaagcagtt ggtaatgttg aaattccaca ggatgctttc ttggctgtat
     2401 tgaaaatgga tgacgaataa gtttttaaaa aatttattaa caatttacaa tgaataaagt
     2461 ttaataacta aaaagaggga gcctaggata aattaacgtc ctgggcttta caatgttata
     2521 ttggcagtca tcgacagagt taaaatgagc ttataacaat ggggccccaa cacagagaat
     2581 ttcgaaaaga aattctacag gcaatgcaag ttggggtgtg ggccccaaca cagagaaatt
     2641 ggattcccaa tttctacaga caatgcaagt tggggtggga caacgaaata aattttgcga
     2701 aaatatcatt tctgtcccac tcccttatgc atgagtttta ctcatgtaat tttattttta
     2761 aggacatatt acatctggct aatgtgtaag agccactaca taataaatca ttagtggttc
     2821 tttattattt ctatctcact ccctctaaac aagaataaat atcaaaatga atcgatatat
     2881 tagacaacca ttgattaaac gttaaagtta aaagtaagaa taacagcaga tagtccaaca
     2941 ggatgtagcc gattggataa acagtctgag aagcggggca ttaaaatgac ggtacaaagt
     3001 gcatatatac atattccatt ttgtgtaaga atatgtacat attgtgattt caataaatat
     3061 tttatacaga atcaacctgt agatgagtac ttagatgcac taataacaga aatgtctaca
     3121 gcaaaatata ggaacttaaa gaccatgtat gtaggtggcg gcacaccaac ggccctttct
     3181 attaatcagt tggaaagatt acttaaagca atacgtgata cgtttacaat cacaggcgag
     3241 tatacatttg aagcaaatcc tgatgagtta actaaagaga aagtccaact attagagaaa
     3301 tatggagtaa atagaatttc aatgggcgtt caaacattca aaccggagtt attgtctgtt
     3361 ttaggtagaa cgcacaatac tgaagatatt tacacttcgg tgttaaatgc taaaaacgca
     3421 ggtattaaat caatcagttt agatttaatg tatcatttac cgaaacagac gattgaagat
     3481 tttgaacaaa gtttagatct agctttagat atggatattc aacatatttc gagttacggc
     3541 ttaatacttg aacctaaaac ccaattttat aatatgtata gaaaagggtt gcttaaactt
     3601 cctaatgagg atttaggtgc tgacatgtat cagttgttga tgtctaagat agaacaatct
     3661 cctttccatc aatacgaaat atctaatttt gcattagatg gccatgaatc agaacataat
     3721 aaggtttact ggtttaatga ggaatattat ggatttggag caggtgcaag tggttatgta
     3781 gatggtgtgc gttatacgaa tatcaatcca gtgaatcatt atatcaaagc tataaataaa
     3841 gaaagtaaag caattttagt atcaaataaa ccttctttga ctgagagaat ggaagaagaa
     3901 atgtttcttg ggttgcgttt aaatgaaggt gtgagtagta gtaggttcaa aaagaagttt
     3961 gaccaatcta ttgaaagtgt ctttggtcaa acaataaata atttaaaaga gaaagaatta
     4021 attgtagaaa agaacgatgc gattgcacta acaaagcgag ggaaagtcat aggtaatgag
     4081 gtttttgaag ctttcctaat aaatgattaa aaaaaattga aatttcgagt ctttaacatt
     4141 gacttacttt gaccaatttg ataaattata attagcactt gagataagtg agtgctaatg
     4201 aggtgaaaac atgattacag ataggcaatt gagtatatta aacgcaattg ttgaggatta
     4261 tgttgatttt ggacaacccg ttggttctaa aacactaatt gagcgacata acttgaatgt
     4321 tagtcctgct acaattagaa atgagatgaa acagcttgaa gatttaaact atatcgaaaa
     4381 gacacatagt tcttcagggc gttcgccatc acaattaggt tttaggtatt atgtcaatcg
     4441 tttacttgaa caaacatctc atcaaaaaac aaataaatta agacgattaa atcaattgtt
     4501 agttgagaat caatatgatg tatcatcagc attgacatat tttgcagatg aattatcaaa
     4561 tatatctcaa tatacaactt tagttgttca tcctaatcat aaacaagata ttatcaataa
     4621 tgtacatttg attcgtgcta atcctaattt agttataatg gttatcgtat tttcatcagg
     4681 tcatgttgag catgtacatc ttgcttcaga cataccgttt agtaatgata aactaaatac
     4741 aatatcaaat tttgttacta acaaactaac tgagtttaat cagaatttac aagatgatat
     4801 tgtctctttt gttcaatcag agcaggaaga aatatttatc aataaattga taaatacgat
     4861 gaataatcat atttctaatc aaagtaatag catttatatg ggtggaaagg ttaaacttat
     4921 tgatgcattg aatgaaagta atgtatcttc aattcagccc attctacaat atatagaatc
     4981 aaatagaatt gcagaattat tacaagatat atcttcacca aatattaatg taaaaattgg
     5041 taatgaaatt gatgacagct taagtgatat ttcaattgta acaagtcaat atcattttga
     5101 tgaaacgtta aaaggtcaaa tcgctgtgat tggacctaca gctatgcatt atcaaaatgt
     5161 cattcaatta ttaaatagaa tttggtaatg atggattaga aagtattgga ggacagaaaa
     5221 tgacaaataa agacgaatca gttgaaaaaa acactgaatc aacagttgaa gaaacaaaca
     5281 tcaaacaaaa tattgatgat tcagttgaac aagctgaaga aagtaaaggt catttacaag
     5341 atgaagcaat agaagaaacg tctgacgaga atgttattga agaaatagat ccaaaagatc
     5401 aaaaaattaa tgaacttcaa caattagcag atgaaaacga agagaaatat ttaaggctct
     5461 acgctgagtt tgaaaattat aagcgtagaa ttcaaaaaga aaatgaaata aataaaacat
     5521 atcaagcaca acgtgtgtta acagatattt taccagcaat agacaatata gaacgtgcac
     5581 ttcaaattga aggtgatgat gagactttta aatctcttca aaaaggtgta caaatggtgc
     5641 atgaaagttt gattaacgca ctaaaagata atggtcttga agttattaaa actgaaggtg
     5701 aagcatttga tccaaatatt caccaagctg tagttcaaga tgataaccct gattttgaat
     5761 ctggcgaaat cactcaagaa ctacaaaaag gatacaagct taaagataga gtattaagac
     5821 catcaatggt caaagtaaac caataactta aatttggcga aaagacattg tttaaaatta
     5881 aattaattta atgattaaat ggaggaattt tattatgagt aaaattattg gtatagactt
     5941 aggtacaaca aattcatgtg taacagtatt agaaggcgat gagccaaaag taattcaaaa
     6001 ccctgaaggt tcacgtacaa caccatctgt tgtagctttc aaaaatggag aaactcaagt
     6061 tggtgaagta gctaaacgtc aagctattac aaacccaaac actgttcaat ctattaaacg
     6121 tcatatgggt actgattata aagtagatat tgaaggtaaa tcatacacac cacaagaaat
     6181 ctcagctatg attttacaaa acttaaaaaa tacagctgaa agctatttag gtgagaaagt
     6241 tgacaaagct gtaattacag tacctgcata ctttaacgac gctgaacgtc aagcaactaa
     6301 agatgctggt aaaattgctg gtttagaagt tgagcgtatc attaatgaac caacagctgc
     6361 agcattagca tatggtttag ataaaactga taaagatgaa aaagttcttg tttttgactt
     6421 aggtggcggt acatttgacg tatctatcct agaattaggt gacggtgtat tcgaagtact
     6481 atcaacagcc ggtgacaaca aacttggcgg tgatgatttt gaccaagtaa ttattgacta
     6541 cctagttgca gaattcaaaa aagaaaatgg cgtagactta tctcaagata aaatggcatt
     6601 acaacgtttg aaagatgctg ctgaaaaagc taaaaaagac ttatcaggtg tatcacaaac
     6661 tcaaatctca ttaccattta tctcagctgg tgaaaacggt ccattacact tagaagtaaa
     6721 cttaactcgt tctaaatttg aagaattatc agattcatta attagaagaa caatggaacc
     6781 tacacgccaa gcaatgaaag acgctggctt aacaaactca gatatcgatg aagttatctt
     6841 agttggtgga tcaactcgta ttccagcagt acaagaagct gtcaaaaaag aaatcggtaa
     6901 agaacctaac aaaggtgtaa acccggacga agtagtggca atgggagctg caatccaagg
     6961 tggcgttatc acaggtgacg ttaaagacgt agtattatta gacgtaacac cactatcttt
     7021 aggtattgaa attttaggtg gacgtatgaa tacgttaatt gaacgtaaca ctacgattcc
     7081 tacatctaaa tctcaaatct attcaacagc agtagataat caaccatcag tagatgtaca
     7141 cgtattacaa ggtgaacgtc caatggctgc ggataataaa acacttggta gattccaatt
     7201 gactgatatt ccaccagctg aacgtggtaa acctcaaatt gaagtaacgt ttgatatcga
     7261 taaaaacggt attgtaaatg taactgcaaa agacttaggt acaaataaag aacaaagaat
     7321 tacaattcaa tcaagttctt cattatcaga cgaagaaatc gaccgtatgg taaaagatgc
     7381 tgaagttaac gctgaagcag ataaaaaacg tcgtgaagaa gtagacttaa gaaacgaagc
     7441 tgacagtcta gtattccaag ttgaaaaaac tttaactgat ttaggcgaaa atattggtga
     7501 agaagataaa aaatctgctg aagagaaaaa agacgctctt aaaactgctt tagaaggtca
     7561 agatatagaa gatattaaat ctaaaaaaga agaacttgaa aaagtgattc aagaattatc
     7621 agcaaaagta tatgagcaag cggctcaaca gcaacaacaa gcacaaggtg caaatgctgg
     7681 tcaaaacaac gatagtactg tagaagatgc tgaatttaaa gaagtaaaag acgacgacaa
     7741 aaaataatta aacaatacaa ttagaatgtt gtcatgatct gaaaagtcaa agtcaataga
     7801 acattggctt tggcttttta tttggatagt atgacatatt acgttaaact atatgagtta
     7861 attaagaaaa ggagagatag ctgtggccaa aagagactat tatgaagttt taggcattag
     7921 taaagatgct tcaaaagatg aaatcaaaaa agcgtatcga aagctttcga aaaaatatca
     7981 tccagatatt aacaaagaag aaggtgcaga tgaaaagttt aaagaaattt ctgaagccta
     8041 tgaagtttta agtgatgata ataaacgcgc aagctatgat caatttggac atgatggtcc
     8101 acaaggtttt ggtggacaag gattcaatgg ctctgacttt ggcggtttta gtggcttcgg
     8161 tggcggcggt tttgaagata tctttagctc tttcttcggt ggcggcagac aaagagatcc
     8221 aaatgcaccg caaaaaggtg atgatcttca gtatacaatg acattaacat ttgaagaagc
     8281 ggtatttggt acaacaaaag aaatttcaat ccgtaaagat gtaacatgcg aaacatgtca
     8341 tggtgatggt gcaaagcctg gaacaagtaa aaagacttgt agttactgta atggcgctgg
     8401 tcatgtagct gtagaacaaa acacaatttt aggtagagtt cgtactgaac aagtttgtcc
     8461 taaatgtaat ggaagtggtc aagaatttga agaagcttgt ccaacatgtc acggaaaagg
     8521 tactgaaaat aaaacagtta aattagaagt gaaagtacct gaaggtgtcg acaatgaaca
     8581 acaaattaga ttagctggtg aaggctctcc aggcgtgaat ggaggacctg ctggtgattt
     8641 atacgtagta tttagagtta aaccatctga aacatttaaa cgtgatggtg atgatattta
     8701 ttacaaatta aatgttagct tcccacaagc agctttaggc gatgaaataa aaataccaac
     8761 attaaataat gaagttatgt taacaatccc tgctggtaca caaacaggta agcaattccg
     8821 cttaaaagaa aagggtataa aaaatgttca tggctatgga tacggagatt tatatgttga
     8881 tattaaagta gtaacgccta ctaaattaac agatagacaa aaagaactaa tgaaagaatt
     8941 tgcccaatta aatggtgaag aaattaatga acaaccttcg aattttaaag atagagcaaa
     9001 aagattcttt aagggagaat aaagtatgaa ctggacagag ctttcaatta ttattaatca
     9061 tgaagcagta gaattggcta ccaatatact tgaaaatcat ggatcaaatg gtgtcgtgat
     9121 agaagattca gatgatttaa ttaaccaacc agaagataaa tacggtgaaa tttacgcttt
     9181 gaaaaaagag gattatccag ataagggagt aagattgaaa gcctatttta atgaaatgac
     9241 ttatgatgat aagttgcgac agcaaattaa agatgagtta ttaaatttag atgaacttga
     9301 tcaacataac attcaattca gtgagcaaat tattgcagag acggattggg aaaatgaatg
     9361 gaaaaactat ttccatccat tccgagcgtc gaagaagttc acaatagttc ctagttggga
     9421 aacatatgct aaagaagcgg atgaagagct ttgcattgag ctcgacccag gtatggcttt
     9481 tggaacaggc gatcatccga ctacaagtat gtgtttgaag gcaatagaaa catatgtatt
     9541 gccacagcat tcagtaattg atgttggtac tggctcaggt atattaagta ttgcaagtca
     9601 tctaatcggt gtaaaacgta ttaaagcgtt ggatattgat gaaatggcag tgagtgtagc
     9661 taaagaaaac ttcagaagaa atcattgtga aacgttaatt gaagctgttc caggtaactt
     9721 attgaaagac gaaacagaaa aatttgatat tgtaatagca aatattttag cgcatattat
     9781 tgatgaaatg attgaagatg cttataatac tctaaatgaa ggcggttatt ttattacttc
     9841 tggtattata aaagagaagt atgaaggtat acagtcacat atggagcgtg taggttttaa
     9901 aattatttca gaacaacatg acaatggttg ggtttgtctt gttggccaga aagtgagtga
     9961 ataatgtgca acgttatttc atagaccaaa acgctgatgt aagtcagcgt ttttttatta
    10021 caaaaaaaga agatattcat catataacaa atgtaatgag atatgatgtt ggtagtaaaa
    10081 ttattttaac ttttaatgat caaacagttt atttatgtga aattgctgac ataacaagtg
    10141 ataaaattgc agttgattta aaagaaaaac aaaatattaa tacagaattg ccagttgatg
    10201 ttacgatttg cagtggacta attaaagctg acaaatatga gtggttgcta caaaaagcta
    10261 ctgaattggg tgcttcatca tttatagctg tgagcatgga acgctcaatt gttaaattaa
    10321 acgaggcgaa agttgtcaaa aagatagagc gttggcaaaa aataattaaa gaagctgctg
    10381 aacaaagcta tcgtttagtg attccatcta ttcaattcga gtcgaattta aaattaattt
    10441 gtgatactat tgataattat gactatattc ttattgcata tgaagaggaa gcaaaagatg
    10501 gtgagttaag caatttcaag caaactttac aacaattcaa ggctcaggat aaagtgttga
    10561 tgatttttgg tcctgagggt ggtttgtcag aaaatgaaat ttcattattt agtaatacta
    10621 gtacagttgt tggtcttgga ccgagaattt tacgggcaga aactgcgcca ttgtatgcgt
    10681 taagtgcaat aagttatgaa aaagaattaa tggggtgaat atttatgtca acagttgcgt
    10741 ttcacacatt aggttgtaaa gtaaaccatt atgaaactga agcaatctgg caattattta
    10801 aagaagcaaa ctatgagcgc gttgactttg aagcgaatgc tgatgtattt gttattaata
    10861 cttgtacagt aacgaataca ggtgataaaa aaagtcgtca aataattaga cgtgcaataa
    10921 gacaaaatcc tgatgctgta atctgtgtaa caggttgtta tgcgcaaact tcatcagctg
    10981 aaattatgga aattcctggt gtcgatgtag tagttggtac acaagataga cataaactat
    11041 taggttacat tgatgaattc cgtaaagaac gccaaccaat taatggtgtt ggaaatatca
    11101 tgaaaaatcg taaatatgaa gaattagatg ttccatattt tacagataga acacgtgcgt
    11161 cattaaaaat tcaagaaggt tgtaacaact tctgcacatt ctgtattatt ccatgggctc
    11221 gtggcttaat gcgttcaaga gatccggaaa aagtagttga acaagcgacg caactagtga
    11281 attcaggata taaggaaatt gtattgacgg gaattcatac aggtggatat ggtcaagatt
    11341 taaaagatta taacttggcc caattattac gtgatcttga aacgattaat ggattagaac
    11401 gaattcgaat ttcttcaatt gaagcaagtc aacttacaga tgaagtaatt gacgttttag
    11461 aacgttcaac aaaagttgtg cgtcatttgc atattccatt acaatctggt tcagatacag
    11521 tattaaaacg tatgagacgt aagtatacaa tggatagatt ttcagaacga ttaacaaaat
    11581 tgcataaagc tttaccagac ttggcagtta cgagtgatgt aattgttggt ttcccaggtg
    11641 aaactgaagc agagttccaa gaaacatatg attttatcgt aaagcataag ttctctgaac
    11701 tgcatgtttt cccttattct cctagaattg gcacgccagc tgcaagaatg gatgaccaaa
    11761 ttgatgaaga aattaaaaat gaacgcgtgc ataagttaat tacgctaagc aatcaactcg
    11821 gaaagttata tgtgtctaaa tttgatcaag atgtgcttga agtaattcct gaggaacagg
    11881 gtgacacaga aggtacatta gttggatatg cagataatta tatgaaagta caatttgaag
    11941 gtgacgaatc actcataggt caaattgtaa aagttaaaat tacgcaagca aattatccat
    12001 taaatgaagg gcaagcaatt aaagttgttg atttcgcaac aaataaatct gatagagaag
    12061 ttttagttta agtattcctt gattcatacc ataaggttcc gtattaaaga tataggtttt
    12121 gtaacgttgt tttaacggct tcacaattta ctatataagc aactcttctc attaaataaa
    12181 ttattaagag atttttagaa caaaatctaa aagtctttgt gattttgatg tgaaatatgt
    12241 ttcaaataaa aaacaattta ctaattgacc ataaattaca gatatattat acttataaat
    12301 gcatagtttt actgtgcaat tgactataaa gttccgttga tatttggagg gagggaaata
    12361 cagatgtcta aaacagtagt acgtaaaaat gaatcacttg aagatgcgtt acgtagattt
    12421 aaacgttcag tttctaaaag tggaacaatc caagaagtac gtaaacgtga attttacgaa
    12481 aaaccaagcg taaaacgtaa aaagaaatca gaagctgcac gtaaacgtaa attcaaataa
    12541 ttaatacctc tgttgactcc ctcaacacga atattaatta tataaaata
//
LOCUS       DANBWH010000068        12512 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.070, whole genome shotgun sequence.
ACCESSION   DANBWH010000068 DANBWH010000000
VERSION     DANBWH010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12512
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.070"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..879
                     /locus_tag="PPY92_001299"
     CDS             <1..879
                     /locus_tag="PPY92_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDJ2625311.1"
                     /translation="ILVVISIFLSLIYSIMTYTFPWSMFVTFIIISIIMLFKHRYLFS
                     HTSSRLFIKKLLSFSYKFVLYTSALLFISTIQSPIVKGLSMWLAILCLSLLLTFICYL
                     VWTVSYRHRSYNKEVDIIIVLGAGIFTEFVTPMLAARLDRALDIYQQQASATKIIVSG
                     GQGPDEPISEALAMQRYLIAHGVDKTDILMESHSINTYTNFLYSKQIINNLYHEAVKI
                     VCVTSQFHVLRALRLAQKLGLSIDGVGSHTPYHFFLHVLIKDYLGVMYQYRLLLTLYC
                     SSSWFICLYAAFIYNS"
     gene            complement(1110..1775)
                     /locus_tag="PPY92_001300"
     CDS             complement(1110..1775)
                     /locus_tag="PPY92_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDJ2625312.1"
                     /translation="MALVVKDIVKNFGEGLSETKVLKGINFEVEQGEFVILNGASGSG
                     KTTLLTILGGLLSQTSGTVLYNDAPLFDKQHRPSDLRLEDIGFIFQSSHLVPYLKVIE
                     QLTLVGQEAGMTKQQSSTRAIQLLKNIGLEDRLNVYPHQLSGGEKQRVAIMRAFMNNP
                     KIILADEPTASLDADRATKVVEMIRQQIKEQQMIGIMITHDRRLFEYADRVIELEDGK
                     ITD"
     gene            complement(1775..2830)
                     /locus_tag="PPY92_001301"
     CDS             complement(1775..2830)
                     /locus_tag="PPY92_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDJ2625313.1"
                     /translation="MKLAIKEIMFYKFRYILITLIILLLSIMVLFISGLAQGLGRENI
                     SLFEHFDNDEYVVQKMKEPQIEKSQLSDTQQNQIKKVIHQEPYKMNIQTLKLSNKEQD
                     VITMNDVKQQRIQLKKGDYPKNAHEVAINDKLAADNIRVGDRLHFKNNSTSYRVSGIL
                     NDTMYAHSSIVLLNDNGFNALNKVNTAFYPVKNLTQQQRDELNKINDVQVVSEKDLTG
                     NIASYQAEQAPLNMMIVSLFAITAIVLSAFFYVMTIQKISQIGILKAIGIKTRHLLSA
                     LVLQILTLTIIGVGIAVIIIVGLSFMMPVTMPFYLTTQNILLMVGIFILVAILGASLS
                     FIKLFKVDPIEAIGGAE"
     gene            2966..3640
                     /gene="hssR"
                     /locus_tag="PPY92_001302"
     CDS             2966..3640
                     /gene="hssR"
                     /locus_tag="PPY92_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding heme response regulator HssR"
                     /protein_id="HDJ2625314.1"
                     /translation="MVQCLVVDDDPRILNYIASHLQTEHIDAYTQPSGEAALKLLEKQ
                     RVDIAVVDIMMDGMDGFQLCNTLKNDYDIPVIMLTARDALSDKERAFISGTDDYVTKP
                     FEVKELIFRIRAVLRRYNINSNSEMTIGNLTLNQSYLELQVSNKTMTLPNKEFQLLFM
                     LAARPKQIFTREQIIEKIWGYDYEGDERTVDVHIKRLRQRLKKLNATLTIETVRGQGY
                     KVENHV"
     gene            3633..5006
                     /gene="hssS"
                     /locus_tag="PPY92_001303"
     CDS             3633..5006
                     /gene="hssS"
                     /locus_tag="PPY92_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme sensor histidine kinase HssS"
                     /protein_id="HDJ2625315.1"
                     /translation="MFKTLYARIAIYSITVILFSALISFVLTNVYYHYNLKASNDAKI
                     MKTLKEARQYEQSAKPTHIQQYFKHLGQMNYQIMTVDQKGHKTFYGEPFREDTLSQNA
                     INNVLNNKDYHGIKDKPFALFVTGFFDNVTDNTVGINFKTKDGSIAVFMRPDIGETFS
                     EFRTFLAVLLMLLLFISISLVIASTYSIIRPVKKLKLATERLIDGDFETPIKQTRKDE
                     IGTLQYHFNKMRESLGQVDQMRQHFVQNVSHEIKTPLTHIHHLLSELQQTSDKTLRQQ
                     YINDIYTITTQLSGLTTELLLLSELDNHQHLLFDDKIQVDQLIKDIIRHEQFAADEKS
                     LIILADLESINFLGNQRLLHQALSNLLINAIKYTDVGGAIDIALQHSHNNIIFTISND
                     GSPISPQAEARLFERFYKVSKHDNSNGLGLAITKSIIELHHGTIQFTQSNEYVTTFTI
                     TLPNNSH"
     gene            5169..5612
                     /locus_tag="PPY92_001304"
     CDS             5169..5612
                     /locus_tag="PPY92_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDJ2625316.1"
                     /translation="MMKLNLFINANETESYIDIHAPKMNDHVQSIINAVNDLDKSHTL
                     VGYIDKEIHIINVSDVITFQVINKNVTAITSNQKFKLKLRLYELEKQLPQHFIRISKS
                     EIVNKYYIEKLLLEPNGLIRMYLKDAHYTYSSRRYLKSIKERLSI"
     gene            5609..6064
                     /locus_tag="PPY92_001305"
     CDS             5609..6064
                     /locus_tag="PPY92_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HDJ2625317.1"
                     /translation="MKNLKNSLFISLIIGLSLSLFFSMLFADGKYYPLNPQSTIGILY
                     YNHFTETTVMLISIILWLLIGVVFFLGDFIFKYTDWSITKATIMHFITTYVGFLPLAM
                     LAGWFPLTVHYLIIFTIIFIVIYVLIWIIQFFKNKNYVDTINEQLKQLK"
     gene            complement(6130..7608)
                     /gene="mqo"
                     /locus_tag="PPY92_001306"
     CDS             complement(6130..7608)
                     /gene="mqo"
                     /locus_tag="PPY92_001306"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HDJ2625318.1"
                     /translation="MTTQHSKTDVILIGGGIMSATLGTLLKELSPEKNIKVFEKLAQP
                     GEESSNVWNNAGTGHSALCELNYTKEGKDGTVDCSKAIKINEQYQISKQFWAYLVKTG
                     QLDNPDRFIQAVPHMSFVIGEDNVAFIKSRVATLKKSVLFEKMKLSQDEEEMKSWVPL
                     MIEGRKSDEPIALTYDETGTDVNFGALTAKLFENLEQRGVGIQYKQNVLDIKKQKSGA
                     WLVKVKDLETNETTTYESDFVFIGAGGASLPLLQKTGIKQSKHIGGFPVSGLFLRCTN
                     QEVIDRHHAKVYGKAAVGAPPMSVPHLDTRFVDGKRSLLFGPFAGFSPKFLKTGSHMD
                     LIKSVKPNNIVTMLSAGIKEMSLTKYLVSQLMLSNDERMDDLRVFFPNAKNEDWEVIT
                     AGQRVQVIKDTEDSKGNLQFGTEVITSDDGTLAALLGASPGASTAVDIMFDVLQRCYR
                     DEFKGWEPKIKEMVPSFGYRLTDHEDLYHKINEEVTKYLQVK"
     gene            complement(7989..9587)
                     /locus_tag="PPY92_001307"
     CDS             complement(7989..9587)
                     /locus_tag="PPY92_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDJ2625319.1"
                     /translation="MLVNTFNPFDNLLLSSLIAAIPIVLFLLCLTVFKMKGIYAAITT
                     LVVTLLIAIPFFKLPVGIASGAVVEGFFQGIIPIGYIVMMAVLLYKITVESGQFLTIQ
                     DSITNISQDQRIQVLLIGFAFNAFLEGAAGFGVPIAICALLLTQLGFNPLKAAMLCLV
                     ANAASGAFGAIGIPVGVVETLKLPGDVSVLGVSQSATLTLAIINFIIPFLLIFIIDGF
                     RGVKETLPAILVVSITYTLTQGLLTVFSGPELADIIPPLLTMLALAVFSKKFQPKHIY
                     RVNKDEEIEPAKAHSAKAVLHAWSPFIVLTVIVMIWSAPFFKNLFLPNGALSSLVFKF
                     NLPGTISEVTHKPLVLTLNIIGQTGTAILLTIIITILMSKKVNFKDAVRLFGVTFKEL
                     WLPVLTICFILAISKITTYGGLSAAMGQGIAKAGNVFPVLSPILGWIGVFMTGSVVNN
                     NSLFAPIQASVAQQIGTSGSLLVSANTVGGVAAKLISPQSIAIATAAVKQVGKESELL
                     KMTLKYSVCLLIFICIWTFILSLL"
     gene            9904..11577
                     /locus_tag="PPY92_001308"
     CDS             9904..11577
                     /locus_tag="PPY92_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDJ2625320.1"
                     /translation="MIKQINVSNMQKFESQLMKAQSEGYTHVVPYANEIMIYQSMLDA
                     VQLYPKSIVVDYTVDGQYKNDCHYFGQSSINIADWAQNNNYYPNLIYAIQQTLDLIHY
                     YSVETIFDLALLTLLKGDLSIDGHVVFDFKAPIATSASIWETIKTIEDFDMMSQFYLN
                     KMAYIDHHPIPFRNLFIEDSEQLNSPDNWLYSTKFMLPKWLYKIAKQRADNKQLQNFG
                     LYTKQPNVLKDHIVFIGDHHQYIGNSKYLFTYFVKHNPMTACYFVTDDRRGPHFISPK
                     SEKADELINSARVVIVENDIPETLQPNGTLIQLHQGTPIMQLFLDSKEPIKNIETPFY
                     RAKRYNRWLQFDYVIHSADDISHFYQTAFPSHQANVLAYGNPKHQYLLQKRNESTTQQ
                     QYKKSFKINDQKPVLLYAPIGLVSAQQLPLSDALFKAYHVVVQGVDEAILPEEALVAP
                     KYLSAQDLILMSDVVITDYSNIIFDAMAIDKTVALYTPNHSQYIESQGVNEDIWRHLS
                     KIWYTDRQLLINNLISQAIPVIKYPQIQHKEQPLESISQLILSKMTSNQ"
     gene            complement(11731..12345)
                     /locus_tag="PPY92_001309"
     CDS             complement(11731..12345)
                     /locus_tag="PPY92_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625321.1"
                     /translation="MKRLVTGLLALSLFLAACGQDSDQQKDSNKEKDDKAKTEQQDKK
                     TNDSSKDKKDNKDDSKDVNKDNKDNSANDNQQQSNSNATNNDQNQTNNNQSSNNQKSS
                     YVAPYYGQNAAPVARQIYPFNGNKTQALQQLPNFQTALNAANNEANKFGSNNKVYNDY
                     SIEEHNGNYKYVFSFKDPNANGKYSIVTVDYTGQAMVTDPNYQQ"
BASE COUNT         4200 a         2203 c         1790 g         4319 t
ORIGIN      
        1 atcttagttg tcatatcgat atttttgtca ctcatctata gcatcatgac ctatactttc
       61 ccttggtcta tgtttgtaac atttataata atcagtatta tcatgctatt caaacataga
      121 tatttatttt ctcatacttc tagtcgctta tttattaaaa aattattatc tttttcatat
      181 aaatttgtac tctacacatc agcacttcta tttataagta cgattcagtc acctattgtg
      241 aaaggtttgt ctatgtggtt agctatatta tgtctttccc tgttactaac ctttatttgt
      301 tatctcgttt ggacagtatc atatagacat agatcataca acaaagaagt tgatatcata
      361 attgtgttag gtgctggtat ttttaccgaa tttgtcacac caatgcttgc agcgcgttta
      421 gaccgtgcac ttgatatcta tcaacaacaa gcatcagcaa caaagattat tgtatctggc
      481 ggacaaggac cagacgaacc aatttctgaa gctttagcta tgcaacgtta tttgattgca
      541 catggtgttg ataaaacaga tatactaatg gaatctcact ctataaatac atacacaaac
      601 tttttatatt ctaaacaaat catcaacaat ttgtatcatg aagcagttaa aattgtttgt
      661 gtaacaagtc agtttcatgt attgcgcgcc ttacgactag cccaaaaatt aggtttatct
      721 atcgatggtg tcggaagtca tacaccatat cactttttct tacatgtgct cattaaagat
      781 tatctaggcg taatgtatca gtatagattg ttattaacgt tatattgttc aagtagttgg
      841 tttatatgtc tttacgccgc ttttatttat aatagttaat ccacaaacaa actattatta
      901 taggttgttt ctaatattgc gtcaaactat ctttaaatat tcgttaatcc ttgattagac
      961 aattgttata ttgaaaataa ataaactaaa gcacgtcatc attttatata atagtgacgc
     1021 gcttttttgt aaacccaaat cgtattctgt tatctaactt ttatactact atgtcttaaa
     1081 tcattaacat ttagcgtctt tacaagccac taatcagtta ttttgccatc ttctaattca
     1141 atcactcgat ctgcatattc aaataatctt cgatcgtgtg taatcataat accaatcatt
     1201 tgttgttctt taatttgttg acgtatcatc tcaacaactt ttgttgctct atcggcatct
     1261 aaacttgctg tgggctcatc tgctaaaatg attttcggat tattcataaa tgctctcata
     1321 atcgcaacac gttgcttttc accgccagat aactgatgcg gatatacatt caagcgatct
     1381 tctaacccaa tatttttcaa aagttgtatt gctcttgttg aactttgttg tttggtcatt
     1441 cccgcttctt ggcctacgag tgtcaattgc tctatcactt ttaaataagg aactaaatgt
     1501 gaagattgaa aaataaaacc aatatcttcc aatcgcaaat cactaggacg atgctgttta
     1561 tcaaacaatg gtgcatcatt gtaaagcact gtaccactcg tttgacttaa caatccgcct
     1621 aatatcgtta gcaatgttgt tttcccagaa ccagaggcac catttaaaat gacaaattcc
     1681 ccttgttcca cttcaaaatt aatacctttt aaaacttttg tttcagacaa accttctccg
     1741 aaatttttga cgatatcttt aacgactaat gccattattc tgcacctcca attgcttcga
     1801 taggatccac tttaaataat ttgataaatg atagtgaggc acctaaaatc gctactaata
     1861 taaatatccc caccattaat aaaatatttt gcgttgttaa gtaaaaaggc atcgttaccg
     1921 gcatcataaa tgatagtcct actatgatga tcacagcaat acctacccca attattgtta
     1981 gtgttaaaat ttgtaaaact aacgcactca ataaatgtct tgtcttaata ccaattgctt
     2041 tcaaaatgcc aatttgtgat attttttgaa tcgtcataac atagaaaaat gcacttagaa
     2101 cgattgctgt aatagcaaac aaactaacaa tcatcatatt taacggtgct tgctctgctt
     2161 gataactcgc aatattacct gttaaatctt tttcactcac aacttgaacg tcatttattt
     2221 tattaagctc atcacgttgt tgttgtgtta aatttttcac tggataaaat gccgtattaa
     2281 ccttattcaa tgcattaaat ccgttatcgt tcaatagcac aatggaacta tgcgcataca
     2341 ttgtgtcgtt taaaatacca gaaactctat aactagttga attattttta aaatgtaatc
     2401 tatccccgac tctaatgttg tctgcagcta acttatcatt aatagcgacc tcatgagcat
     2461 ttttaggata atcacctttt ttaagttgta tgcgttgttg tttaacgtca ttcattgtaa
     2521 tcacatcttg ttctttatta gataatttca atgtttgaat attcatttta tatggttctt
     2581 gatgaattac ctttttaatt tgattctgtt gcgtatcact aagttgcgat ttctcaattt
     2641 gcggctcttt cattttttga acaacatatt catcattatc aaagtgttca aataacgaaa
     2701 tattctccct accaagccct tgagctaaac cactaataaa taacaccata atacttaata
     2761 aaagaatgat taatgtgatt aaaatataac gaaatttgta aaacataatc tcttttatcg
     2821 ctaatttcat atcgattcac ttctccctat ttcttcttta gcgtcagtat aaggttgaaa
     2881 tatgaactca atatgaacaa ttgttttttt acgcaaaaat attttacatg tataataaat
     2941 gaaaaagata agggagttta tagctatggt gcaatgtctt gttgtcgatg acgatcctcg
     3001 aatcctaaat tatatagcta gccatttaca aacagagcac attgatgcat acacacaacc
     3061 aagtggtgaa gctgcattaa aacttttaga aaaacagcgt gtcgatattg cagtggtaga
     3121 tattatgatg gatggtatgg acggctttca attatgtaat acattaaaaa atgattatga
     3181 tataccagtt attatgttaa cagcgcggga tgcacttagt gacaaagagc gtgcgtttat
     3241 aagtggtact gacgattatg taaccaaacc ctttgaggtt aaggaactta tttttagaat
     3301 tcgtgcggta ttacgtcgat ataatatcaa ttcaaattca gaaatgacta ttggcaactt
     3361 aacgctaaac caatcctatt tggaactcca agtatctaat aaaacgatga cgttaccgaa
     3421 caaggaattt caattattat ttatgcttgc agcacgtcct aagcaaatct ttactcgtga
     3481 acaaataata gaaaaaattt ggggctatga ttatgaagga gatgaacgaa cagttgacgt
     3541 tcatattaag cgactacgcc aaagattaaa aaaattaaat gccacactta caattgaaac
     3601 agtaagagga caaggctata aggtggagaa tcatgtttaa aacactctat gctagaattg
     3661 cgatttattc cattacggtt attttattta gtgcattaat aagttttgta ttaacaaatg
     3721 tttactatca ttataattta aaagcatcta atgacgcgaa aatcatgaag acacttaaag
     3781 aagcaagaca atatgaacaa tcagctaaac caacgcacat tcaacaatat ttcaaacatt
     3841 tagggcaaat gaattaccaa ataatgaccg ttgatcaaaa aggtcataag acattttatg
     3901 gggagccatt tagagaagat actttatcac aaaatgctat taacaacgta ttaaataata
     3961 aagattacca tggtattaaa gataaaccat tcgcattatt cgtaactgga tttttcgaca
     4021 atgtaacaga taatactgtt ggtattaact ttaagacaaa agacggttca atagcagtat
     4081 tcatgcgtcc agatattgga gaaacattta gcgagtttag aacattctta gccgtcttgt
     4141 taatgttgtt attatttatc tcgatttcat tagttatcgc atcaacctat tcaatcattc
     4201 gtccagtaaa aaagttaaag ctagcgaccg aacgcttaat tgatggtgat tttgaaacac
     4261 ctatcaaaca aacacgcaaa gatgaaattg gaacattaca gtatcacttt aataagatga
     4321 gagagtcatt gggacaagtt gatcaaatga ggcagcattt tgtacaaaat gtatctcatg
     4381 aaatcaaaac accattaaca catattcatc atttattaag cgagttacaa caaacttctg
     4441 ataaaacatt aagacaacaa tatataaatg atatttatac cattacaacg cagctgagcg
     4501 gattaacaac tgaattgtta ctcttatccg aattagataa tcaccaacat ttattattcg
     4561 atgataagat tcaagtcgat caactcatta aagatatcat tcgacatgaa caatttgctg
     4621 ctgatgaaaa gtcgttaatc attttagctg acctagaatc aatcaatttc ctaggaaatc
     4681 aacgtttgtt acatcaagca ttgagtaatt tattaataaa tgctattaag tatacagatg
     4741 ttggtggtgc cattgacatc gctttgcaac atagtcacaa caatatcatt ttcacaataa
     4801 gtaatgatgg ttcaccaatt agtccacaag ctgaagcacg tttatttgaa cgtttttata
     4861 aagtgagcaa acatgataat agcaatggtt taggtcttgc aattacaaaa tccatcattg
     4921 aattgcatca tggtacaatc caattcacac aaagtaacga gtatgtaacc acctttacta
     4981 ttacactgcc aaataattca cattaatctc tacctcctga aaaaacattt ctgtttttat
     5041 ttcaggaggt atcttttata ttgattagcg ctttctagcc acttaacatt taatttctac
     5101 cattcaacaa aatttaaata tatcgatggt catttatggt ttaataattt tgaaggaggc
     5161 attaacctat gatgaaactc aatttattta tcaatgcaaa tgagacggaa tcttatattg
     5221 atatacatgc acctaaaatg aatgatcacg ttcaaagtat tattaatgca gtcaatgatt
     5281 tagacaaatc acatacatta gtcggataca tagacaaaga aatccatatc attaatgtat
     5341 cagatgttat aacattccaa gttattaata aaaatgtaac agccattaca agcaatcaaa
     5401 aattcaaact aaaattacga ctttatgaac tagaaaaaca gttaccacaa catttcattc
     5461 gcatttccaa atcagaaatc gtcaacaagt attacattga aaaattatta ttagagccaa
     5521 atggacttat acgtatgtat cttaaagatg cacactacac ctattcttct agacgttatt
     5581 taaaatctat aaaggagcgc ttatcaatat gaaaaatttg aaaaactcat tatttattag
     5641 cttaatcatt ggtttgtccc tttcactgtt ctttagtatg ttatttgcag acggcaaata
     5701 ttatccactg aacccacaat caactattgg cattttgtat tataatcatt tcacagaaac
     5761 taccgtaatg ttgatttcta tcattttatg gttactcata ggcgtcgtct ttttccttgg
     5821 agattttatc tttaaataca cagattggag cattactaaa gcaactataa tgcatttcat
     5881 aacaacgtat gtcggattct tgcctttagc aatgcttgcc ggttggtttc cactaacagt
     5941 gcattatcta atcatattca caattatctt cattgtgatt tatgtattaa tttggataat
     6001 tcaattcttt aaaaacaaga actacgtaga cactattaac gagcaactga aacaattaaa
     6061 atgattttta ttaaccacta aaaaacggta atgtctatca cattaaaaaa gacattaccg
     6121 tttcgtttat tatttaactt gtaaatactt agttacttct tcattaattt tgtgatataa
     6181 atcctcatga tctgttaagc gataaccaaa tgacggcacc atttctttaa tctttggttc
     6241 ccaccctttg aattcatcac gatagcatcg ctgtaaaaca tcaaacataa tatctacagc
     6301 tgttgacgca ccaggtgatg caccaagtaa tgcagctaat gtgccatcat ctgacgtaat
     6361 gacttcggta ccaaattgta agttaccttt agaatcctca gtatccttga ttacttggac
     6421 acgttgccct gctgtaatca cttcccaatc ttcattttta gcatttggga aaaagactct
     6481 taaatcatcc atacgctcat cattagataa catcaattgt gacactaaat acttcgtaag
     6541 actcatttct ttgatacctg cagataacat cgtcacgata ttatttggtt taaccgattt
     6601 aattaaatcc atatgtgaac ctgtttttaa aaatttaggt gagaaacctg caaatggacc
     6661 aaataacaat gaacgcttgc cgtctacaaa acgtgtatct aagtgcggca ctgacattgg
     6721 tggcgcaccc actgctgctt ttccgtacac tttagcatga tgacgatcaa tcacttcttg
     6781 atttgtacag cgcaggaata atccacttac tgggaaacca ccaatatgtt ttgattgttt
     6841 aatcccagtc ttttggagta atggtaaact cgcaccgcca gcaccaataa atacaaaatc
     6901 agattcatat gttgtcgttt cattagtttc taaatcttta actttaacta gccatgcccc
     6961 agatttctgt ttcttgatgt ctaatacatt ctgcttatat tgaattccca caccacgttg
     7021 ctctaaattt tcaaataact ttgcagttaa cgcaccaaag ttaacatctg taccagtttc
     7081 atcataagtt aaagcaattg gttcatcaga cttacgacct tcaatcatta acggtaccca
     7141 agatttcatt tcttcttcat cttgcgataa tttcattttt tcgaataaaa cacttttctt
     7201 taacgttgca acacgacttt ttataaaagc tacattatct tcgccaatga caaaactcat
     7261 gtgtggcacc gcttgaataa agcgatctgg gttatctaat tgtcctgttt taactaaata
     7321 tgcccaaaac tgttttgaaa tttggtactg ctcatttatc ttaattgctt tactacaatc
     7381 aactgtgcca tccttacctt cttttgtata gttcaactcg caaagtgctg aatgccctgt
     7441 accggcatta ttccatacat ttgaactctc ttcgccaggt tgtgctaatt tttcaaacac
     7501 tttaatattt ttctcaggtg ataattcttt aagtaatgtt cctaatgttg cactcataat
     7561 accgccacca attaagatga catctgtttt gctatgttgt gttgtcataa caaatacagt
     7621 cccccttttt aaaatgaata atggaacgtt cgagaaatac gacatattaa taccacttta
     7681 aatcaataag tgtctcaata ggttcccatg gtgatgctct attactatct actatgtata
     7741 ttaatatttt ataataacga aactacttta attatatact ttatgtgcga tagcacaaag
     7801 acaaaaccac aaaccttaac agctatcaac caatttaatt tgattgctga ttactcacta
     7861 ttcattttaa cataaagcgc taccattatt ataatgtcaa acttctttca ttaatgatcc
     7921 ataaaaaaaa gatgagatgt tagaaaacat ctcaccttct gtatatttat attttgaaat
     7981 acgttttttt ataataatga caagatgaaa gtccaaatac agatgaatat tagtaaacat
     8041 acactgtatt tcaatgtcat ttttaataat tctgattcct taccaacttg ttttactgct
     8101 gcagttgcaa ttgcaattga ttgtggtgaa atcaattttg ccgctacacc accaactgta
     8161 ttagcagata caagaagtga accacttgtt ccaatttgtt gtgcaacaga agcttgaatt
     8221 ggtgcaaata atgaattgtt atttacaact gatcctgtca taaacacacc tatccaacct
     8281 aagattggtg atagaactgg gaagacatta cctgctttag ctataccttg acccattgct
     8341 gcacttaaac caccataagt tgtgatttta gaaattgcta agatgaaaca aattgtaaga
     8401 actggtaacc acaactcttt aaatgtaacg ccgaataatc taacagcatc tttaaagtta
     8461 acctttttag acattaaaat tgtaataata atagttaata aaatagctgt acctgtttgt
     8521 ccaataatat ttaaagtcaa tactaatggt ttatgcgtaa cttcgctgat tgttccaggt
     8581 aagttgaatt taaatactaa tgatgataaa gcaccatttg gtaagaataa gtttttaaag
     8641 aatggcgcac tccaaatcat tacaatgact gttaatacaa tgaatgggct ccatgcatgt
     8701 aatactgctt ttgcagaatg tgcttttgca ggttcaattt cttcatcttt attaacacga
     8761 taaatgtgtt ttggttggaa ttttttagaa aatactgcta atgctaacat tgttaataac
     8821 ggtggaataa tatctgctaa ttcaggtcca ctgaatacag ttaataatcc ttgagtaagt
     8881 gtataagtga ttgaaactac taaaattgct ggtaatgttt ctttaacacc tctaaaacca
     8941 tcaataataa agataagtaa gaatggaata atgaaattta tgattgccaa tgttaaagtt
     9001 gctgattgag aaacacctaa tactgaaaca tctccaggta atttcaacgt ttctacaaca
     9061 cctacaggga taccaatcgc accaaaagca ccagacgctg catttgcgac taaacataac
     9121 atcgcagctt ttaatggatt aaatcctaat tgtgttaata aaagtgcaca aattgcaatt
     9181 ggtacaccaa atcctgctgc accttctaaa aatgcgttga atgcaaatcc aataagtaaa
     9241 acttgaatac gttggtcttg tgaaatattt gtaatactat cttgaattgt taaaaattgt
     9301 ccagattcaa cagtaatttt gtataacaat actgccatca taacgatata gccaatcgga
     9361 atgatacctt ggaagaaacc ttcgactact gcaccagaag cgataccaac tggcaatttg
     9421 aaaaatggta ttgcaattag taatgttaca acaagtgttg taatagctgc ataaatacct
     9481 ttcattttaa aaacagttaa acatagtaaa aacagtacaa tcggtattgc tgcaattaaa
     9541 gacgataata ataaattatc aaatgggtta aacgtattta ctaacatagg ctgactcttc
     9601 tttccataaa gtagtagtgt aattgcttca ttaaaatcat gctctggcta tagcattttg
     9661 ttatcgataa cattttacag aacagtgaga ttaatgtgat ttttttcaca attaaaatat
     9721 accatttttt cagactatta acaatatcac tttcctattt aattgtttta tttttcattt
     9781 taaatctgtt catcaaaatg aattgtactt aatgtaatga agtaacttat actattcatg
     9841 aattttacat aagaccgtgc taaaataagg aagatactac gatgagaaat ggaagttata
     9901 gcaatgatta aacagataaa tgtttctaat atgcaaaagt ttgaatctca attaatgaaa
     9961 gctcagtctg aaggttatac acatgtcgtt ccatatgcaa atgagataat gatttatcaa
    10021 tcgatgttag atgcggttca attatatcct aagtctatcg tggttgatta tactgtggat
    10081 ggacaataca aaaatgattg tcactatttc ggtcaatcat cgatcaacat tgccgattgg
    10141 gctcaaaaca ataattatta tcctaacctg atatatgcga ttcaacagac tcttgattta
    10201 atacattatt actctgtaga aacgattttc gatttagctt tattgacact tctgaaaggt
    10261 gatttatcaa tcgatggtca tgtcgttttt gattttaaag cccctatagc aacaagtgct
    10321 tcaatatggg aaactattaa aactattgaa gattttgata tgatgtctca gttttatttg
    10381 aataaaatgg cttatattga tcatcatcct ataccttttc gcaacctttt tatcgaggat
    10441 tcagaacaac ttaattcgcc agataattgg ttgtattcta ccaagtttat gctacctaaa
    10501 tggttatata aaatagcaaa gcaacgtgct gataacaaac aattacaaaa cttcggcctg
    10561 tatacgaagc aaccaaacgt tttaaaagat cacatcgttt ttatcggtga tcatcatcaa
    10621 tatataggta attcaaaata tttatttact tacttcgtta aacataatcc aatgactgcc
    10681 tgttattttg ttactgacga tcgtcgcgga ccacatttca tatcacctaa gtctgaaaaa
    10741 gctgacgaat tgattaacag cgcacgcgta gttatagttg aaaatgatat acctgaaacg
    10801 ttacaaccaa acggtacttt aattcaatta catcaaggca caccaataat gcaattattt
    10861 ttagatagta aagagcctat taaaaatata gaaacacctt tttatcgtgc taaaaggtat
    10921 aatcgctggt tgcaattcga ttatgttata cattctgctg atgatattag ccacttttat
    10981 caaacggctt ttcctagtca ccaagcaaat gtattggctt atggtaatcc taaacaccaa
    11041 tatttactac aaaagcgtaa cgaaagtact actcaacaac agtataagaa atcgtttaaa
    11101 ataaatgatc aaaaaccagt tttattatac gcacctattg gattagtgag tgcgcaacaa
    11161 ttaccactat cagatgcttt attcaaagcc tatcatgttg tggttcaggg tgttgatgaa
    11221 gcgatcttac cagaagaggc gcttgttgca ccaaagtatc ttagtgctca agacttaatt
    11281 ctaatgtctg atgtcgttat cactgattac tcaaatataa tatttgatgc gatggctata
    11341 gacaaaacag tagcattata cactccaaat cacagtcaat acattgagtc tcaaggtgtt
    11401 aacgaagata tttggcgtca tctatcaaaa atttggtata cagaccgaca attattaatt
    11461 aataatttaa tttcccaggc aataccagtg attaaatatc cacaaataca acataaagaa
    11521 caaccattag aatctatttc acaacttata ttgtctaaga tgacatctaa tcaatagtgt
    11581 ttaaatttct cagtggctgt gaatgaggtt taaaagtgct ataaaacgta aacttttata
    11641 ctttaaaata cgcaaaaaac ggtaaaccct aattcatatt atagagttta ccgttttatt
    11701 tttttaactt gcatcatagt tatattaaca ttattgttgg tagtttggat cagtaaccat
    11761 tgcttgtcca gtataatcaa ccgttacaat tgaatatttt ccatttgcat ttgggtcttt
    11821 aaaactaaac acatacttat agttgccatt atgttcttca atagaataat cattatacac
    11881 tttattatta ctaccaaatt tatttgcttc attattagcc gcatttaaag ctgtttggaa
    11941 atttggcaat tgctgtaaag cttgagtttt atttccatta aacggataaa tttgacgtgc
    12001 aaccggcgcg gcattttgtc cataatatgg tgcaacgtaa cttgattttt gattattact
    12061 tgattggtta ttatttgttt ggttttggtc attgtttgtt gcatttgaat tagattgttg
    12121 ctggttatcg tttgcactat tatctttatt atctttgttt acgtctttac tatcatcttt
    12181 attgtctttc ttatctttag atgaatcatt tgttttttta tcttgttgtt cagttttcgc
    12241 tttatcatct ttttctttat tactgtcttt ttgttggtca ctatcttgac cacatgcagc
    12301 taaaaataat gataatgcta gtaaccctgt aactaatctt ttcatacata tctcctccta
    12361 taattcgata ttcattgaat aagcttgaaa tacatatcta ccatgtgtat catttcatgg
    12421 cttgttacca aagacgtcaa cattaatatg atttgaatta tcacaatacc cataactaca
    12481 atctaaggtt ctttttatta taccctaatt tt
//
LOCUS       DANBWH010000069        12091 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.071, whole genome shotgun sequence.
ACCESSION   DANBWH010000069 DANBWH010000000
VERSION     DANBWH010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12091
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.071"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..416
                     /locus_tag="PPY92_001310"
     CDS             <1..416
                     /locus_tag="PPY92_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDJ2625322.1"
                     /translation="GSLFFNDLNIFESSGNQLSLLNQLLLVSLGTFVGVWLVLSLTLL
                     LSSATNSTGVAIAVGIVFYFASSILAVIQTALLEKIDWLKWNPINMMNIMLQTVEKGF
                     SKSTKLELHELFIGNIAYISIFLILVVFIFKKKNI"
     gene            789..2753
                     /locus_tag="PPY92_001311"
     CDS             789..2753
                     /locus_tag="PPY92_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HDJ2625323.1"
                     /translation="MTQITEKELKKKYLDLLSQNFDTPEKLATEIINLESILELPKGT
                     EHFVSDLHGEYEAFQHVLRNGSGNVRAKINDIFKERLSTKELNDLTALVYYPEDKLKL
                     IKSDFQSCGQLNVWYITTIEHLIELIKYCSSKYTRSKLRKALPKQYVYIIEELLYKSN
                     EYQNKKSYYETLVNQVIELKQADDLIIGLAYSVQRLVVDHLHVVGDIYDRGPQPDKIM
                     DTLINYHSLDIQWGNHDVLWVGAYAGSKVCLANLLRICARYDNLDIIEDAYGINLRPL
                     LTLAEKYYDADNPAFKPKKRPDKHERLTQREESQITKIHQAIAMIQFKLEIPIIKRRP
                     NFEMEERLVLEKVNYDTNEITVYGNTYPLKDTCFQTINRNNPAELLPEEEEVMNKLLL
                     SFQQSEKLRRHMSFLMRKGSLYLPYNGNLLIHGCIPVDENGEMESFEIDGHTYSGQEL
                     LDVFEYHVRKSFDEKENTDDLSTDLVWYLWTGKYSSLFGKRAMTTFERYFIADKASHK
                     EEKNPYYHLREDVNMVRKMLSDFGLNPDEGRIINGHTPVKEINGEDPIKADGKMLVID
                     GGFSKAYQSTTGIAGYTLLYNSFGMQLVAHQQFNAKEKILSEGIDELSIKRVVDKELQ
                     RKKIRDTNIGKELQAQIDILKMLMHDRYLD"
     gene            3095..4174
                     /locus_tag="PPY92_001312"
     CDS             3095..4174
                     /locus_tag="PPY92_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625324.1"
                     /translation="MRILKESIIVAFAFVGVVVGAGFATGQEIFQFFTSHGAYSISGI
                     IVTGLLITLGGMVVMHTGHHLKSRNHSDSINYFLYPSIARGFDIILTMFMLSLAIIMT
                     AGGASTIHQSFNLPYWLSALILVAFILATLFLKFDRLIAVLGGVTPFLIAIVIMIAVY
                     YFTTSHLDFTAANNDAQIHKQKSLSPGWWFDAINYASLQIAAAFSFLSVMGSKVKYRD
                     STLYGGLIGGLIITFLLMMINLGLISQFDKIKHVDLPTLKLATQMSPSIGIIMSVIMI
                     LVIYNTVVGLMYAFASRFSVPFSRRYFIIIITMAVITYISTFIGFISLIGKVFPIMGL
                     FGFILLIPVLYKGLIKRITGKSHID"
     gene            complement(4279..4872)
                     /locus_tag="PPY92_001313"
     CDS             complement(4279..4872)
                     /locus_tag="PPY92_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDJ2625325.1"
                     /translation="MEHIFREGQKGAPTLILLHGTGGDEFDLLPLGEALNENYHLLSI
                     RGQVSENGMNRYFKRLGEGVYDEEDLEFRGQELLAFIKEAAERYEFDIEKAVLVGFSN
                     GSNIAINLMLRSEAPFKKALLYAPLYPVEVTSTKDLSDVSVLLSMGKHDPIVPLAASE
                     QVINLFKTRGAVVEEVWVNGHEITETGLTAGQQILGK"
     gene            complement(4903..5862)
                     /locus_tag="PPY92_001314"
     CDS             complement(4903..5862)
                     /locus_tag="PPY92_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001628197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HDJ2625326.1"
                     /translation="MINNHELLGIHHVTAMTDDAERNYKFFTEVLGMRLVKKTVNQDD
                     IYTYHTFFADDVGSAGTDMTFFDFPNITKGQAGTNSITRPSFRVPNDDALMYYEQRFD
                     EFGVKHEGIQELFGKKVLPFEEVDGQVYQLISDELNDGVAPGVPWKNGPVPVDKAIYG
                     LGPIEIKVSYFDDFKNILETVYGMTTIAHEDNVALLEVGEGGNGGQVILIKDDKGPAA
                     RQGYGEVHHVSFRVKDHDAIEAWATKYKEVGINNSGIVNRFYFEALYARVGHILIEIS
                     TDGPGFMEDEPYETLGEGLSLPPFLENKREYIESEIRPFNTKR"
     gene            complement(5940..6374)
                     /locus_tag="PPY92_001315"
     CDS             complement(5940..6374)
                     /locus_tag="PPY92_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDJ2625327.1"
                     /translation="MDRTKQSLNVFVGMNRALDTLEQITKEDVKRYGLNITEFAVLEL
                     LYNKGPQPIQRIRDRVLIASSSISYVVSQLEDKGWITREKDKDDKRVYMACLTEKGQS
                     QMADIFPKHAETLTKAFDVLTKDELTILQQAFKKLSAQSTEV"
     gene            6600..6884
                     /locus_tag="PPY92_001316"
     CDS             6600..6884
                     /locus_tag="PPY92_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDJ2625328.1"
                     /translation="MSNLEIKQGENKFYIGDDENNALAEITYRFVDNNEINIDHTGVS
                     DELGGQGVGKKLVKAVVEHARENHLKIIASCSFAKHMLEKEDSYQDVYLG"
     gene            7055..7861
                     /locus_tag="PPY92_001317"
     CDS             7055..7861
                     /locus_tag="PPY92_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDJ2625329.1"
                     /translation="MAFHDKTATQVTNIELNVRDLDLMTTFYKNILGLSVKSSDDNTT
                     VLSVGTGGHTLTLHLLEDGRQTSPREAGLFHIAFLLPTTEDLANFLYFVAQKNMGIGA
                     GDHLVSEALYFNDPEGNGIEVYRDRPSSSWEWQNGKVKMDTLEVDSQTLLTHRTDEGW
                     QGMPAKGMIGHLHLKTHDLDAAYQCYIEQLGFQHVSDFPRALFMSTNHYHHHIAANTW
                     QSNQARQNNAQSYGLTHVDIYQPDASTHTFTAPEGFDITVHSNTDLVPEK"
     gene            complement(7982..8653)
                     /locus_tag="PPY92_001318"
     CDS             complement(7982..8653)
                     /locus_tag="PPY92_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDJ2625330.1"
                     /translation="MSNMNQTIMDAFHFRHATKQFDPQKKVSKEDFETILESGRLSPS
                     SLGLEPWKFVVIQDQALRDELKAHSWGAAKQLDTASHFVLIFARKNVTSRSPYVQHML
                     RDIKKYEAQTIPAVEQKFDAFQADFHISDNDQALYDWSSKQTYIALGNMMTTAALLGI
                     DSCPMEGFSLDTVTDILANKGILDTEQFGLSVMVAFGYRQQDPPKNKTRQAYEDVIEW
                     VGPKE"
     gene            8923..9915
                     /locus_tag="PPY92_001319"
     CDS             8923..9915
                     /locus_tag="PPY92_001319"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDJ2625331.1"
                     /translation="MTKIMFFGTRDYEKEMALNWGKKNNVEVTTSKELLSSATVDQLK
                     DYDGVTTMQFGKLENDVYPKLESYGIKQIAQRTAGFDMYDLDLAKKHNIVISNVPSYS
                     PETIAEYSVSIALQLVRRFPDIERRVQTHDFTWQAEIMSKPVKNMTVAIIGTGRIGAA
                     TAKIYAGFGATITAYDAYPNKDLDFLTYKDSVKEAIKDADIISLHVPANKESYHLFDK
                     AMFDHVKKGAILVNAARGAVINTPDLIAAVNDGTLLGAAIDTYENEAAYFTNDWTNKD
                     IDDKTLLELIEHERILVTPHIAFFSDEAVQNLVEGGLNAALSVINTGTCETRLN"
     gene            complement(10162..10962)
                     /locus_tag="PPY92_001320"
     CDS             complement(10162..10962)
                     /locus_tag="PPY92_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDJ2625332.1"
                     /translation="MVKAIAVDMDGTFLDSKKTYDKPRFEAIFTELRNRDITFIAASG
                     NQYAKLKSIFGDRDMYFISENGAVIYKGNELYNYKSFNRQVFQQVVDYLNMNQSIDQL
                     VICGLKSAYILKHTSEAFKEDTRFYYHQLKEIDSLQQLPEDDYVKIAFNINRETHPNV
                     DEEVATQFSNDIKLVSSGHDSIDIIMPNMTKGQALKRLLDKWEMSPSELMAFGDANND
                     KDMLAFAKHSYVMENSHDEELFNIASAVAPSNDKQGVLTIIEQEVLNK"
     gene            11282..11917
                     /locus_tag="PPY92_001321"
     CDS             11282..11917
                     /locus_tag="PPY92_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDJ2625333.1"
                     /translation="MKLNNYSLKVKNKQLVDNCDLNFYLGQINHIVGKNGVGKSLLAK
                     DFLLNNSGNIPKSISQNVTLISSSSNIPNDITKDFLLSLLKSKFENNRQTFDKIYNIL
                     NIEAIPSNVLLKNLSDGQKQKLKLLSFLLEDHDLIILDEVTNALDKKTVNEIYEFLND
                     FIQSHQTKTIINITHNLSDLSALPGKYFIFKDLQIEEYQSKEEVINDYINL"
     gene            11923..>12091
                     /locus_tag="PPY92_001322"
     CDS             11923..>12091
                     /locus_tag="PPY92_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HDJ2625334.1"
                     /translation="MSTLKLEFKKSISNKIIYTLGVLFIFLFLLGYFLPIGIDKVKNL
                     SYGQFFFSSYTV"
BASE COUNT         3937 a         2135 c         1860 g         4159 t
ORIGIN      
        1 ttgggtcttt attctttaat gatttaaata tatttgaaag tagcggtaat caattatctt
       61 tattgaatca attattatta gttagtttag gtacatttgt tggcgtttgg ttagttttaa
      121 gcttaacgtt gctattatca tctgcaacta attcaacggg tgtagccatt gctgtaggta
      181 ttgtttttta ttttgcaagt tctattttag cagttattca aacggcactt ttagaaaaaa
      241 tagactggct aaagtggaat cctattaata tgatgaatat tatgcttcaa acagttgaaa
      301 aaggctttag taagtcgacg aaattagaac ttcatgaatt gtttattggt aatattgctt
      361 atatttctat tttcttaata cttgtagtat ttattttcaa gaagaaaaat atttagtaac
      421 ttaaagtatt agatgaataa atacacacat attccatcgt aattcaaaat cattttcaaa
      481 tcccttcacc caaataatgg tgcggggatt tttttatcca aattttggca attccacatt
      541 ttacatatcg taatttttta ggaaactagt gaatataaca aattcctcct ctcattttta
      601 aaataaatat atcactttcc cacttttact taactaaact gcaacgactc ctaataccaa
      661 aatcctgccc tctattttta tcaatccaag catacttatt gaaaaatgtt aacgttttct
      721 tgataatcat tgtaagcgca tttattttat aaactaacgt ttgaaatata ctacaggagt
      781 gacacgtaat gactcaaatt actgaaaaag aattaaaaaa gaagtattta gatttactat
      841 cccaaaattt tgatactcca gaaaaacttg caactgaaat tatcaattta gaatcaattt
      901 tagaattacc taaaggtacg gaacatttcg tcagcgattt acatggtgaa tacgaagctt
      961 tccaacacgt attacgcaac ggttctggga atgtgcgagc gaaaatcaat gatattttca
     1021 aagagagact ttcaactaag gagcttaatg atttaactgc tcttgtctac tatccagaag
     1081 acaaattaaa attgattaaa agtgatttcc aaagttgcgg tcaacttaat gtctggtata
     1141 tcacaacaat cgaacattta attgagttga ttaaatattg ttcttcaaaa tatacgcgtt
     1201 caaaactgcg taaagcattg ccaaaacaat acgtttatat tattgaggaa ctactgtata
     1261 aaagtaatga atatcaaaat aaaaaatcat attacgaaac acttgttaac caagtaattg
     1321 aacttaaaca ggcagatgat ttaattattg gactcgctta ttccgtacaa cgcttagtcg
     1381 tcgatcattt acacgttgtc ggtgatattt atgatcgtgg accacaacca gataaaatta
     1441 tggatacact gattaattat cattccctag atattcaatg gggtaatcat gatgtgcttt
     1501 gggttggagc ctatgctggg tcaaaagtat gcttagcaaa cttacttcga atttgtgcac
     1561 gctatgataa tttagatatt atcgaagacg cttatggcat taatttaaga ccactgctta
     1621 ctttagctga aaaatactat gacgcagata atcctgcttt taagcctaaa aaaagacctg
     1681 acaaacacga acgtttaact caacgtgaag aaagtcaaat tactaaaatt catcaagcta
     1741 ttgcgatgat tcaattcaag ttagaaatac caattattaa acgtcgtcca aatttcgaaa
     1801 tggaagaacg tcttgtgctt gaaaaggtta attatgatac aaatgaaatt acagtttatg
     1861 gtaatacata cccgttgaaa gacacatgtt tccaaactat caatcgtaat aatccagcag
     1921 aattactacc tgaagaagaa gaagtcatga ataaactatt attgtcattc caacaatctg
     1981 aaaaattacg tcgtcatatg tctttcttga tgcgtaaagg ttcgctttac ttaccatata
     2041 atggtaattt actcattcat ggttgtattc cagttgatga aaatggtgag atggaatcat
     2101 ttgaaattga tggtcatact tacagcggcc aagaattatt agatgtgttt gagtatcatg
     2161 tccgtaaatc atttgatgaa aaagaaaata ctgatgactt atcgacggat ttagtttggt
     2221 atttatggac tgggaaatat tcgtcattat ttggtaaacg cgcgatgact acgtttgagc
     2281 gatattttat tgcagataaa gcttctcata aagaagaaaa aaatccatac tatcatttac
     2341 gtgaagatgt gaatatggtt cgtaaaatgc tcagtgattt cggattaaat ccagatgaag
     2401 gacgcattat taatggtcac acaccagtga aagaaatcaa tggcgaagat cctatcaagg
     2461 ctgatggaaa gatgcttgtc attgatggtg gtttttcaaa agcttatcag tcaacaactg
     2521 gcattgcagg ctatacattg ttgtataatt cattcggaat gcaactcgtt gcacaccaac
     2581 aatttaacgc taaagaaaaa atactttccg aaggtatcga tgaactttct ataaagcgcg
     2641 ttgtagacaa agaattacaa cgtaagaaga ttcgtgatac caatattggt aaagagcttc
     2701 aagctcaaat agatattttg aaaatgttaa tgcatgatcg ttacttagac taaagacgac
     2761 attttatgca tgtattgtat actgttgtat tgatgcggtt tacgcattgg ctacgcaaac
     2821 gcgatgataa ataggcgacc tagctacacc gaaaaaaatc ccctcactat tgcgagtagt
     2881 gaggggattg gtgtatctat gctaattcct gttaactgta ttataacaac tttacagcaa
     2941 aaaaatgcaa aatcaactaa taaattaatg aaatgaccga cgccctcgct tctacttcaa
     3001 tttaacaaaa tactatatta gtactatatt tttcattaac atgtatgtta cattttctat
     3061 attacttaaa attacgaagg gacattttta aatcatgcgt atcttaaaag agtccattat
     3121 tgtggcattt gcctttgttg gtgttgtcgt tggtgccggc tttgctactg gtcaagaaat
     3181 tttccagttt ttcacaagtc atggcgcata tagcatttca ggcattattg taacaggact
     3241 attgattact ttaggtggaa tggttgtcat gcatacaggt catcatctaa agtccagaaa
     3301 tcattctgat tcaattaact atttcttata cccctctatt gcaagaggtt ttgatattat
     3361 tttaacaatg tttatgttgt ctttagctat tattatgact gcaggtggtg cgtcaaccat
     3421 tcatcaaagt ttcaacttac cgtattggct gagcgcactc atattagtcg cctttatttt
     3481 agcaacactg tttctaaaat tcgatcgttt aattgctgtg cttggtggag ttactccatt
     3541 tttaattgca attgtcatta tgattgcggt ctactatttc acaacgagtc atcttgattt
     3601 tactgccgct aataatgatg ctcagattca taagcagaaa tcattatcac ctggatggtg
     3661 gtttgatgcg attaactatg caagcttgca aattgctgct gccttcagct tcttatcagt
     3721 gatgggtagc aaagttaaat atcgtgactc aacgttatac gggggcttga ttggcggttt
     3781 aatcattaca tttttactca tgatgattaa cctaggttta atttctcaat tcgataaaat
     3841 taaacacgtc gatttaccta cattaaaatt agcgacacaa atgtctccgt caattggtat
     3901 tattatgtct gtcattatga tacttgtcat ctacaatact gttgttggat taatgtatgc
     3961 atttgcgtca cgtttcagcg ttccattcag cagacgttac ttcatcatta ttattacaat
     4021 ggctgtcatc acttatatta gtacatttat cggtttcatt tcattaattg gaaaagtatt
     4081 ccctattatg ggattgttcg gtttcatctt actcatacct gtactctata aaggtttaat
     4141 taagcgtatt accggcaaat ctcatatcga ttaaaacatc tatactattg taaaatttat
     4201 tacagcactt caaaatcaaa aaagcgggca gatgaaaagt aaaaatcttt tccatctgtc
     4261 cgcttcttaa tagaactgtt atttcccaag tatttgttga ccagccgtta atccagtttc
     4321 tgtaatttca tgaccattca cccaaacttc ttcgactact gccccacgtg tcttaaacaa
     4381 gttaatgact tgttcacttg cagctaatgg cacaattgga tcatgtttcc ccatagaaag
     4441 caacacactt acatctgata aatcctttgt tgacgttact tcaactgggt ataacggtgc
     4501 atataacaat gcttttttaa atggtgcttc cgaacgcaac attaagttaa tcgctatatt
     4561 tgatccattt gaaaatccaa caagtactgc tttttcaata tcaaattcat aacgttcagc
     4621 agcttcttta atgaacgcta acaattcttg tccacgaaat tccaaatctt cttcatcata
     4681 aacaccttca ccaagacgtt tgaaataccg gttcatccca ttttctgaaa cttgtcctct
     4741 aatacttaac aagtgataat tttcattcaa tgcttcgcct aatggcaata aatcgaattc
     4801 atcgccacct gtaccatgca acaatattag cgttggtgca cctttttgtc cttcacgaaa
     4861 aatatgttcc atcacaaatc ctcctcattc caattaatca tgttaacgct tagtattaaa
     4921 tggtcttatt tctgattcta tatactctct tttattttct aaaaatggtg gcaatgataa
     4981 accttcacct aatgtttcat acggttcatc ttccataaat cctggtccat ctgttgaaat
     5041 ttctattaaa atatgcccca cacgtgcata taatgcttca aaatagaaac gattaacgat
     5101 gcctgagttg ttaataccta cctctttata tttcgttgcc cacgcttcta ttgcatcatg
     5161 atctttcaca cgaaatgaca catgatgtac ctcaccataa ccttgacgtg ctgctggccc
     5221 tttatcatct tttattaaga ttacctggcc accattgccg ccttcgccaa cttcaagtaa
     5281 tgcgacatta tcttcatgcg caatagttgt cataccgtaa acagtctcta aaatattttt
     5341 aaagtcgtca aaataactta ctttaatttc aatgggtcct aatccataaa tcgctttatc
     5401 tactggaacc ggtccattct tccaaggtac accaggtgct accccatcat ttaactcatc
     5461 tgaaattaat tgatacactt ggccatcgac ttcttcaaat ggcaacactt ttttaccaaa
     5521 taattcttga ataccttcgt gtttaacacc aaattcatca aagcgctgtt cataatacat
     5581 taatgcgtca tcgttaggca ctctaaaaga cggtcttgta atggaatttg ttcctgcctg
     5641 cccttttgta atatttggaa aatcaaagaa tgtcatatct gtacctgctg aacctacatc
     5701 atctgcaaaa aatgtatgat acgtataaat atcatcttga ttgactgtct ttttaactaa
     5761 acgcatgcct aatacttctg taaaaaattt ataattacgt tctgcatcat ctgtcattgc
     5821 tgtgacatgg tgaataccta gtaattcatg attatttatc attttatata ccctctttat
     5881 atttttagtg tatttatctc gaaatcgaga tattttaatg taaattttta gtgcacgcct
     5941 tacacttctg tagattgtgc acttagtttc ttaaacgctt gttgtaagat tgttaattca
     6001 tcctttgtta acacatcaaa cgcttttgtt aatgtctcag catgcttagg gaaaatatct
     6061 gccatttgac tttgaccttt ttcagttaaa caagccatat atacacgttt atcatcttta
     6121 tccttttcac gtgtaatcca acctttgtcc tctaattgac ttacaacata tgaaatgctg
     6181 ctacttgcaa ttaatacgcg gtctctaata cgttgaattg gttgcggacc tttattataa
     6241 agcaactcga gcactgcaaa ttcagtaata tttaagccat atcgctttac gtcttctttt
     6301 gtaatttgct ctaatgtgtc taacgcccta ttcattccga caaagacatt gagcgattgt
     6361 ttcgttcgat ccatatcatc accaactttt taatttattt cgatttcgag ataaattata
     6421 tagctgaatg gatttaatgt caagatacgt gttttcatct ttatttcaaa ttgcgcttaa
     6481 taaaaacgac tacataaatg cggcaatgtc gtcacatgac tgtcatgcac cttttacttc
     6541 gtttaagtta aaatagaata gggaaatatt ataatcaaac atgataggag ttgatatgta
     6601 tgagtaacct tgaaatcaaa caaggcgaga acaaattcta tattggtgat gatgaaaata
     6661 atgctttagc tgaaatcaca taccgttttg tggataataa tgaaattaac attgatcata
     6721 caggcgtatc tgatgaactt ggtggtcaag gtgttggcaa aaaactagtt aaagcagttg
     6781 ttgaacatgc tcgagaaaat catttaaaaa ttattgcctc atgttcattt gccaaacata
     6841 tgttagaaaa agaagattca tatcaagatg tatatcttgg ttaaataaat tttgcgatgg
     6901 tgcaactgta ccatcgcttt tttgttcgca tcaatataaa attgcaaaac gactagttat
     6961 tttgcatttc aaactttatg ctatcaattc attttctttt ctggtatact ttttatacta
     7021 acttatagaa aaatagaaaa cgaggcgatg cataatggca tttcacgata aaacagcaac
     7081 acaagtgaca aacattgaac tgaacgtaag agacttagac ttaatgacaa cattttacaa
     7141 aaacatttta ggactatccg ttaaaagttc tgacgataac acaaccgtat tatctgttgg
     7201 gactggtggc catactctaa cgttacattt attagaagac ggccgtcaga cttccccacg
     7261 tgaagcagga ctttttcata tagcattttt attaccaact actgaggatc tagctaactt
     7321 cttatatttc gtggcacaaa aaaatatggg cattggcgct ggtgatcatt tagtaagtga
     7381 agctttatat ttcaacgacc ccgaaggtaa tggtattgaa gtctatcgcg atagaccttc
     7441 atcttcatgg gaatggcaaa atggcaaagt taaaatggat actttagaag ttgatagcca
     7501 aaccttatta acacatcgta ctgatgaagg ttggcaagga atgccagcaa aaggcatgat
     7561 tggacactta catttaaaaa cacatgattt agacgcagca tatcaatgtt acattgaaca
     7621 attaggattc caacatgtgt ctgattttcc acgtgcgcta tttatgtcaa cgaatcatta
     7681 tcaccatcat attgctgcta atacttggca atccaatcaa gcaagacaaa acaatgcaca
     7741 aagttatgga ttaacacatg ttgatattta tcaacccgac gcctcaacac atacatttac
     7801 ggcaccagaa gggtttgaca tcacagttca ttcaaatact gaccttgttc cagaaaaata
     7861 atcatccata ctttattaat acaatttttt cacgcaaaaa cctgcgattc atttatgtaa
     7921 tcgcaggctt tttttattgc taacttttta attttatata tttagacata ctgtattcta
     7981 tttattcttt tggtccaacc cattcaataa catcttcata tgcttggcgt gttttatttt
     8041 tcggtggatc ttgttgtctg tagccaaatg caaccatcac tgataaacca aattgctcag
     8101 tatctaagat acctttattt gctaaaatgt ctgtcactgt atccagacta aaaccttcca
     8161 tcggacatga atcaatacct aacaatgcgg ctgtcgtcat catattgcct aatgcgatat
     8221 acgtttgttt acttgaccag tcatacaagg cttgatcatt atcagaaata tggaaatctg
     8281 cttggaatgc atcgaatttt tgttcaacag ctgggatcgt ttgtgcctca tattttttaa
     8341 tatctcttaa catatgttgt acatacggtg atcttgacgt tacattttta cgcgcaaaaa
     8401 ttagcacaaa atggctcgct gtatctaatt gttttgctgc gccccaactg tgcgctttta
     8461 attcatcacg taacgcttga tcttgaatca cgacaaactt ccaaggttct aacccaagag
     8521 aacttggaga caatctacct gactctaata ttgtttcaaa atcttctttc gaaactttct
     8581 tttgtggatc gaattgctta gtcgcatgtc tgaaatgaaa tgcatccata attgtttgat
     8641 tcatattgct cattgaacat agcctccaat ttttatattt attactatac aaattatact
     8701 tcatgtttta aaaagactaa agtaatccgt ttgcaattcg ctataacttg tgcacatgtt
     8761 ataagttttc tgcaataaaa cacacttttg aattttggaa aatttagtgt tataatatat
     8821 ttgtgatatt tttcacaatt aaattgtggc gtgattttaa tgtacataat ctttaatttc
     8881 actcatttac ttttcaatct tttaaaagcg aggtttttaa taatgacaaa aattatgttc
     8941 tttggtacgc gtgattatga gaaagagatg gcattaaatt gggggaaaaa gaataatgtc
     9001 gaagtaacta cttctaaaga gctattatca agtgctacag tcgatcaatt aaaagattac
     9061 gatggcgtaa ctacaatgca atttggtaag ttagaaaatg acgtttatcc taaattagaa
     9121 tcttacggta ttaaacaaat tgcacaacgt actgctggat ttgatatgta tgatttagat
     9181 ttagctaaaa aacacaacat tgtgatatct aacgttccta gttattcacc tgaaacaatt
     9241 gcagagtatt ctgtttctat cgccctacaa ttagtgcgtc gcttcccaga tattgaacgc
     9301 cgtgtacaaa cacatgattt tacttggcaa gcagaaatca tgtctaaacc agttaaaaat
     9361 atgactgttg caattatcgg tacgggtcgt atcggtgctg ctacagctaa aatatatgca
     9421 ggatttggtg ctacaattac agcttatgac gcttatccta ataaagattt agacttttta
     9481 acttataaag atagtgttaa agaagctatt aaagatgccg atattatttc tttacatgtt
     9541 ccagcgaaca aagaaagcta tcatttattc gataaagcaa tgtttgatca tgtgaaaaaa
     9601 ggtgcaatct tagttaacgc agcacgtggt gcagtcatca atacacctga tttaatcgct
     9661 gcagtgaacg atggtacttt gttaggtgct gcgattgata cttatgaaaa tgaagctgca
     9721 tacttcacaa atgactggac taataaagac attgacgata aaacattatt agagttaatc
     9781 gaacatgaaa gaattttagt aacaccacat attgctttct tctctgatga agcagtacaa
     9841 aaccttgttg aaggtggttt aaacgcagca ttatctgtca ttaacactgg tacatgtgaa
     9901 acacgtttaa attaatatat atttaaaagc aaaggcgcac catgtgcctt tgctttttat
     9961 tttgtttatc tgtatcaaaa tcggtttgat taaaatctgc tttattttat catctattca
    10021 actgattgta gtgcgcttat tttactatgg cattattgcc taacctgttt gagatatatt
    10081 aatgtttgat gattcgtatt caaagttttc atttaacatg tatttagtat catgatagcc
    10141 gcttcattga tgatgtgcta cctacttgtt caatacttct tgttcgataa tcgttaaaac
    10201 accctgctta tcattacttg gagcgacagc cgaggcaata ttgaacaatt cttcatcatg
    10261 actattttcc atcacataac tatgcttagc gaacgctagc atatctttat cattattcgc
    10321 atctccaaag gccatgagct ctgaaggaga catttcccat ttatctaaca atcgttttaa
    10381 tgcctgccct ttagtcatgt ttggcataat aatatcaatg ctatcgtgcc cacttgagac
    10441 aagtttaata tcattgctga attgcgttgc tacttcttcg tcaacatttg gatgcgtctc
    10501 acgattaata ttaaatgcta ttttgacata atcatcctca ggtaattgtt gtagactgtc
    10561 aatttctttt aactgatgat aataaaatct cgtatcttct ttaaatgctt cagaagtatg
    10621 ttttaaaata tacgcgcttt tcaaaccaca gatgacgagt tgatcaatac tttggttcat
    10681 atttaagtaa tcgacgacct gttgaaacac ctgacgatta aagcttttat aattatataa
    10741 ctcattgcct ttataaataa ctgcaccatt ttcagaaata aaatacatat ctctatcccc
    10801 gaaaatagac ttcaacttcg catattgatt gccactcgca gcaataaatg taatatctct
    10861 atttctaagt tcagtaaaaa tcgcttcaaa tctcggttta tcgtatgtct ttttcgaatc
    10921 taaaaacgtc ccgtccatat ctaccgcaat agccttaacc atgattatcc tcctcattgc
    10981 tcttttagtc gtcttcatta tatccatctc atgacaagca atgaagtttg tcataccaca
    11041 atgaaactat attcacattt tgaaattgct ttcatatatg caaaacacat tacactggat
    11101 atagcattcg gaggcgaaca taaatttcag aatattgtaa aaatataacg atatatttat
    11161 catttggaca aaatatagtg tgatatacta atttataaca tctatacata atgtgaaaaa
    11221 atttttaaaa tacaattaaa tatcggtgta ataaatccct ttatacatag gagttgtata
    11281 catgaaacta aataactatt ctttaaaagt taaaaacaaa caactagttg acaattgtga
    11341 tttaaatttc tatcttggtc agatcaatca cattgttggt aaaaatggtg taggaaaatc
    11401 tttattagct aaagatttct tactaaataa tagtggaaat atccctaagt ccatttctca
    11461 aaatgtaacc ttaatatcaa gttcatcaaa tattcctaat gatataacaa aagatttttt
    11521 attatcattg ttaaaatcaa aatttgaaaa caatcgacaa acattcgata agatttataa
    11581 catactaaac atcgaagcaa taccgtctaa cgtactacta aaaaacttga gtgatggtca
    11641 aaaacaaaag cttaaattat taagtttctt attggaagat catgatttaa tcatattaga
    11701 tgaagttaca aacgctttag acaagaaaac agttaatgaa atttatgaat ttttaaatga
    11761 ttttattcaa agccatcaaa ctaaaactat tatcaatatt acacataatt tatccgattt
    11821 aagtgctttg ccgggtaaat actttatttt taaagacctt caaatagaag agtaccaatc
    11881 aaaagaagaa gtcataaatg attacattaa tttataattg aggtgtccac tttgaaatta
    11941 gaatttaaaa aaagtatttc caataaaatt atttacacgc ttggtgtgct attcatattc
    12001 cttttcttac ttgggtattt tttaccaata ggaatagaca aagttaaaaa tttaagctat
    12061 ggtcagtttt tctttagttc ttatacagtt g
//
LOCUS       DANBWH010000070        12023 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.073, whole genome shotgun sequence.
ACCESSION   DANBWH010000070 DANBWH010000000
VERSION     DANBWH010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12023
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.073"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..771
                     /locus_tag="PPY92_001323"
     CDS             <1..771
                     /locus_tag="PPY92_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDJ2625335.1"
                     /translation="IFVILIPKLTGEIPSYINFGLMNYQNASYLSAFTAGLGIYFIMK
                     GSVKHKWIYVLFTIIDIPIVFIPGGRGGAILLILYGLFAFILITFKRGIPIAVKSIMY
                     IFALSISSVLIYFLFTKGSNTRTFSYLQGGTLNLEGTSGRGPIYEKGIYFIQQSPLLG
                     YGPFNYYKLIGNIPHNIIIELILSFGLLGFFIIMICILLLVYKMIRNYDPNTIDLLVM
                     FIAIYPITLLMFSSNYLVVSEFWFVLFYFITKGRRHHG"
     gene            764..1969
                     /locus_tag="PPY92_001324"
     CDS             764..1969
                     /locus_tag="PPY92_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HDJ2625336.1"
                     /translation="MVKKVFIMDSVKTIIGTLLIALGLQFLAYPIINQRVGNEAFGSI
                     LTIYTIITITSVVLGNTLNNIRLINMNLYKSNHYYWKFVSILLISILIESIALIIVFL
                     YFFNLNTIDIIFLILLNILMCLRIYLNVFFRMTLKYNQILYIALIQFLGLLIGLFLYY
                     LIQNWIVCFITSELFATIYTLVKLRGLTIGEYQSEDNNVVKDYVMLLSTNSLNNLNLY
                     LDRLILLPIIGGTAVTISFLSTFIGKMLATFLYPINNVVLSYISVNESDNIKKQYLKT
                     NLFAIAALCLVMIICYPITLIIVSLLYNIDSSLYSKFIILGNIGVLFNAVSIMIQTLN
                     TKHASITLQANYMTLHTITFIFITILMTIAFGLNGFFWTTLFSNIIKYVILNIIGLKS
                     KFINKKDVD"
     gene            1970..3175
                     /gene="cap8L"
                     /locus_tag="PPY92_001325"
     CDS             1970..3175
                     /gene="cap8L"
                     /locus_tag="PPY92_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8L"
                     /protein_id="HDJ2625337.1"
                     /translation="MSEKKILILCQYFYPEYVSSATLPTQLAEDLIANHINVDVMCGW
                     PYEYSNHKQVSKTEMHRGIRIRRLKYSRFNNKSKVGRIINFFSLFSKFVINIPKMLKY
                     DQILVYSNPPILPLIPDVLHRLLKKKYSFVVYDIAPDNAIKTGATRPGSMIDKLMRYI
                     NRHVYKNAENVIVLGTEMKNYLLNHQISKNADNIHVIPNWYDMRQLQDNRIYNDTFKA
                     YREQYDKILLYSGNMGQLQDMETLISFLKLNKDQPQTLTILCGHGKKFADVKTAIEDH
                     RIENVKMFEFLTGTDYADVLKIADVCIASLIKEGVGLGVPSKNYGYLAAKKPLVLIMD
                     KQSDIVQHVEQYDAGIQIDNGDAHAIYNFINTHSSKELHEMGERAHQLFKDKYTREIN
                     TMKYYNLLK"
     gene            3186..3743
                     /gene="cap8M"
                     /locus_tag="PPY92_001326"
     CDS             3186..3743
                     /gene="cap8M"
                     /locus_tag="PPY92_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8M"
                     /protein_id="HDJ2625338.1"
                     /translation="MKRLFDVVSSIYGLVVLSPILLITALLIKMESPGPAIFKQKRPT
                     INNELFNIYKFRSMKIDTPNVATDLMDSTSYITKTGKVIRKTSIDELPQLLNVLKGEM
                     SIVGPRPALYNQYELIEKRTKANVHTIRPGVTGLAQVMGRDDITDDQKVAYDHYYLTH
                     QSMMLDMYIIYKTIKNIVTSEGVHH"
     gene            3743..4630
                     /gene="capN"
                     /locus_tag="PPY92_001327"
     CDS             3743..4630
                     /gene="capN"
                     /locus_tag="PPY92_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     epimerase CapN"
                     /protein_id="HDJ2625339.1"
                     /translation="MRKNILITGVHGYIGNALKDKLIEQGHQVDQINVRNQLWKSTSF
                     KDYDVLIHTAALVHNNSPQARLSDYMQVNMLLTKQLAQKAKAEDVKQFIFMSTMAVYG
                     KEGQVGKSDQIDTQTPMNPTTNYGISKKFAEQALQELISDSFKVAIVRPPMIYGAHCP
                     GNFQRLMQLSKRLPIIPNINNQRSALYIKHLTAFIDQLISLEVTGVYHPQDSFYFDTS
                     SVMYEIRRQSHRKTVLINMPSVLNKYFNKLSVFRKLFGNLTYSNTLYENNNALEVIPG
                     KMSLVIADIMDETTTKDKA"
     gene            4684..5946
                     /gene="cap8O"
                     /locus_tag="PPY92_001328"
     CDS             4684..5946
                     /gene="cap8O"
                     /locus_tag="PPY92_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8O"
                     /protein_id="HDJ2625340.1"
                     /translation="MKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQSGQI
                     SIEEPGLQEVYEEVLSSGKLKVSTTPEASDVFIIAVPTPNNDDQYRSCDISLVMRALD
                     SILPFLEKGNTIIVESTIAPKTMDDFVKPVIENLGFTIGEDICLVHCPERVLPGKILE
                     ELVHNNRIIGGVTKACIEAGKYVYRTFVQGEMIETDARTAEMSKLMENTYRDVNIALA
                     NELTKICNNLNINVLDVIEMANKHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKL
                     IQTGREINNSMPAYVVDTTKQIIKALSGNKVTVFGLTYKGDVDDIRESPAFDIYELLN
                     QEPDIEVCAYDPHVELDFVEHDMSHAVKDASLVLILSDHSEFKNLSDSHFDKMKHKVI
                     FDTKNVVKSSFEDVSYYNYGNIFNFIDK"
     gene            6023..7168
                     /gene="cap8P"
                     /locus_tag="PPY92_001329"
     CDS             6023..7168
                     /gene="cap8P"
                     /locus_tag="PPY92_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8P"
                     /protein_id="HDJ2625341.1"
                     /translation="MKKIMVIFGTRPEAIKMAPLVKEIDHNGNFEANIVITAQHRDML
                     DSVLSIFDIQADHDLNIMQDQQTLADLTANALAKLDSIINEEQPDMILVHGDTTTTFV
                     GSLAAFYHQIPVGHVEAGLRTHQKYSPFPEELNRVMVSNIAELNFAPTVIAAKNLLFE
                     NKDKERIFITGNTVIDALSTTVQNDFVSTIINKHKGKKVILLTAHRRENIGEPMHQIF
                     KAVRDLADEYKDVVFIYPMHRNPKVRAIAEKYLSGRNRIELIEPLDAIEFHNFTNQSY
                     LVLTDSGGIQEEAPTFGKPVLVLRNHTERPEGVEAGTSRVIGTDYDNIVRNVKQLIED
                     DEAYQRMSQANNPYGDGQASRRICEAIEYYFGLRSDKPDEFVPLRHK"
     gene            complement(7233..7559)
                     /gene="isdI"
                     /locus_tag="PPY92_001330"
     CDS             complement(7233..7559)
                     /gene="isdI"
                     /locus_tag="PPY92_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdI"
                     /protein_id="HDJ2625342.1"
                     /translation="MFMAENRLQLQKGSAEETIERFYNRQGIETIEGFQQMFVTKTLN
                     TEDTDEVKILTIWESEDSFNNWLNSDVFKEAHKNVRLKSDDDGQQSPILSNKVFKYDI
                     GYHYQK"
     gene            complement(7566..7949)
                     /locus_tag="PPY92_001331"
     CDS             complement(7566..7949)
                     /locus_tag="PPY92_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HDJ2625343.1"
                     /translation="MRLILIVIGLIFTALGIAGAVLPLLPTTPFLLVAVFCFARSSDR
                     FYNWLINQKIYKEYVENFYLHRGYTLQQKIKILISLYIVIGFSIYMVDVLAVRVGLII
                     MVIIQTAVLFTFVKTLPKSNHKIEE"
     gene            8376..9863
                     /locus_tag="PPY92_001332"
     CDS             8376..9863
                     /locus_tag="PPY92_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDJ2625344.1"
                     /translation="MAVNVRDYIAENYGLFINGEFVKGSSDETIEVTNPATGETLSHI
                     TRAKDKDVDHAVKVAQEAFESWSLTSKSERAQMLRDIGDKLMAQKDKIAMIETLNNGK
                     PIRETTAIDIPFAARHFHYFASVIETEEGTVNDIDKDTMSIVRHEPIGVVGAVVAWNF
                     PMLLAAWKIAPAIAAGNTIVIQPSSSTPLSLLEVAKIFQEVLPKGVVNILTGKGSESG
                     NAIFNHDGVDKLSFTGSTDVGYQVAEAAAKHLVPATLELGGKSANIILDDANLDLAVE
                     GIQLGILFNQGEVCSAGSRLLVHEKIYDQLVPRLQEAFSNIKVGDPQDEATQMGSQTG
                     KDQLDKIQSYIDAAKESDAQILAGGHRLTENGLDKGFFFEPTLIAVPDNHHKLAQEEI
                     FGPVLTVIKVKDDQEAIDIANDSEYGLAGGVFSQNITRALNIAKAVRTGRIWINTYNQ
                     VPEGAPFGGYKKSGIGRETYKGALSNYQQVKNIYIDTSNALKGLY"
     gene            10510..11469
                     /locus_tag="PPY92_001333"
     CDS             10510..11469
                     /locus_tag="PPY92_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDJ2625345.1"
                     /translation="MNGKKANTINRYKYFHHVNHQKIQQSSKKTLWASLIITLLFTVI
                     EFVGGLVSNSLALLSDSFHMLSDVLALGLSMLAIYFASKKPTARYTFGYLRFEILVAF
                     LNGLALIVISIWILYEAIVRIIYPQPIESGIMFMIASIGLLVNIILTIILVRSLKQED
                     NINIQSALWHFMGDLLNSIGVIVAVVLIYFTGWRIIDPIISIVISLIILRGGYKITRN
                     AWLILMESVPQHLDTDQIMADIKNIDGILDVHEFHLWSITTEHYSLSAHVVLDKKYEG
                     DDYQAIDQVSSLLKEKYGIAHSTLQIENLQLNPLDEPYFDKLT"
     gene            complement(11764..11967)
                     /locus_tag="PPY92_001334"
     CDS             complement(11764..11967)
                     /locus_tag="PPY92_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625346.1"
                     /translation="MDKESVVASLARNKKIAVETMAGQRYIIERILHTNDEKHIHILK
                     PKDVVLDVDSIKEIDENHLNDAT"
BASE COUNT         4131 a         1731 c         2196 g         3965 t
ORIGIN      
        1 atttttgtaa ttttaatacc aaaacttaca ggtgagatac ctagctatat caattttgga
       61 cttatgaact atcaaaacgc ttcgtacctt tcagcattta ctgccggatt aggcatttat
      121 ttcattatga aaggttcagt gaaacataag tggatatatg ttctatttac aataattgat
      181 atccctattg tgtttatacc aggagggcgt ggaggtgcta ttttattaat tctttacggc
      241 ttatttgcat ttatacttat tacgtttaaa agaggaatac ctattgcagt aaaaagcatt
      301 atgtatattt ttgcattaag catatctagt gtattgattt actttctttt tacaaaaggt
      361 tcgaatacta gaacattttc atatctacaa ggtggaacac ttaatttaga aggtacttct
      421 ggaagaggac cgatttatga aaaaggtatt tactttattc aacaaagtcc gttattaggc
      481 tatgggccat ttaactatta taaactaatc ggaaatatac cacataacat cattattgag
      541 ttgattctat catttggctt attagggttt tttatcataa tgatttgcat tttgctacta
      601 gtttataaaa tgattaggaa ctatgatcca aacactatag atttactcgt tatgtttata
      661 gcaatctatc caatcacatt attaatgttt agttcaaatt atttagttgt aagtgaattt
      721 tggtttgtgt tgttctattt tattacaaaa ggacggcgtc atcatggtta agaaagtttt
      781 tattatggat agcgtaaaga caataattgg tacgttgctt atagctttag gattacaatt
      841 tttagcttat ccaattatta atcaacgagt aggtaatgaa gcgtttggtt ctattttaac
      901 gatttataca ataataacaa tcacgagtgt tgtattaggc aatacgctta acaatatacg
      961 attaattaat atgaatctat acaaatccaa tcattactac tggaaatttg tgtcgatact
     1021 tttaatttca attctgattg agagtatagc tttaattatt gtatttcttt acttttttaa
     1081 tttgaacacc atcgatatta tctttttaat tctacttaat attttaatgt gtttaaggat
     1141 ttatctgaat gtatttttta ggatgacttt aaaatataat cagattttgt atattgctct
     1201 tattcaattt ttaggtttgc tgataggact atttctatat tatttaatcc aaaactggat
     1261 tgtttgtttt attaccagtg aattgtttgc aacgatatat acattggtta aattacgggg
     1321 attaactata ggcgagtatc aaagtgaaga taataatgtg gtcaaagatt atgtgatgct
     1381 actgagtaca aatagcctta ataatttgaa tctctactta gatagattaa tcttattacc
     1441 aattataggt ggaacagctg taactatatc atttctttca acatttattg ggaaaatgtt
     1501 agctacattt ctgtatccga ttaataatgt agtactttca tatatttctg taaatgaaag
     1561 cgacaatata aagaagcaat atttgaaaac taatctattt gctatagctg cactatgttt
     1621 agtcatgatt atatgttatc caattacatt aattattgtc tctttactgt ataacattga
     1681 ttcaagttta tattcgaagt ttattatttt aggtaatata ggtgttttat tcaatgcagt
     1741 gagtattatg atccaaactt taaatacaaa acacgcatca ataacattac aagcgaatta
     1801 tatgacgctt cacacgatta catttatatt cataactatt ttaatgacaa ttgcgtttgg
     1861 tctaaatgga ttcttttgga caacgctgtt cagcaacatt attaagtatg tgattttaaa
     1921 tattataggt ttaaagtcta aattcattaa taaaaaggac gtcgattaga tgagtgaaaa
     1981 aaagattttg attttatgtc agtattttta tccggaatat gtatcttctg cgacgttacc
     2041 aactcaattg gcggaagatt taattgcgaa tcacattaat gtcgatgtca tgtgtggatg
     2101 gccatatgaa tatagtaatc ataaacaggt ttctaaaacc gagatgcatc gtggaattcg
     2161 cattcgacgt ctcaagtatt cgaggtttaa taacaaaagt aaggttggaa ggatcatcaa
     2221 tttctttagt ttattttcaa aattcgtgat taatatacct aaaatgttga aatatgatca
     2281 gattcttgtt tactctaatc caccaatctt gccattaata ccagacgttt tacacagact
     2341 gcttaagaaa aaatattctt ttgtggtgta tgatatagca cctgataatg cgattaagac
     2401 aggtgcaact cgtccaggta gcatgattga taagctgatg cgttacatta atagacatgt
     2461 ctacaagaat gctgaaaatg tcattgtcct tggtacggaa atgaaaaact acttactaaa
     2521 tcatcaaatt tctaaaaatg ctgacaatat ccatgtgatt cctaactggt atgacatgcg
     2581 tcaattacaa gacaatcgta tctataatga cacatttaaa gcttaccgtg agcaatacga
     2641 caaaatttta ttgtatagcg gtaatatggg gcagttacag gatatggaga cacttatctc
     2701 atttttaaaa ttaaataagg atcagcctca aacgttaaca atactttgtg gtcatggtaa
     2761 gaaatttgca gatgtcaaaa cggcaataga agaccatcgt attgaaaatg ttaaaatgtt
     2821 tgagttttta acaggtacag actatgctga cgtattaaaa attgcggatg tatgtattgc
     2881 atcgctgatt aaagaaggcg tcggtttagg cgttccgagc aagaattatg gctaccttgc
     2941 agctaagaaa ccgttggtac tcatcatgga taagcaatct gatatcgttc aacatgttga
     3001 acaatatgat gcgggtatcc aaattgataa tggcgatgca catgccattt ataacttcat
     3061 caacactcac tcgagtaagg aattgcacga gatgggtgag cgcgcacatc aactgtttaa
     3121 agataaatat acgagagaaa ttaatactat gaagtattac aatctgttga agtgaggaga
     3181 taattatgaa gcgattattc gatgtagtga gttcaatata tggtttagta gttttaagtc
     3241 cgattctgtt aattacagca ttactaatta aaatggaatc acctggacca gccattttca
     3301 aacaaaaaag accgacgatt aataatgaat tgtttaatat ttataagttt agatcaatga
     3361 aaatagacac acctaatgtt gcaactgatt taatggattc aacatcgtat ataacaaaga
     3421 cagggaaggt cattcgtaag acctctattg atgaattgcc acaattattg aatgttttaa
     3481 aaggagaaat gtcaattgta ggtcctagac cagcgcttta taatcaatac gaattaatcg
     3541 aaaaacgtac aaaagcgaac gtgcatacga ttagaccagg tgtgacagga ctagctcaag
     3601 tgatggggag agatgatatt actgatgatc aaaaagtagc gtatgatcat tattacttaa
     3661 cacatcaatc tatgatgctt gatatgtata tcatatataa aacaattaaa aatatcgtta
     3721 cttcagaagg tgtgcatcac taatgagaaa aaatatttta attacaggcg tacatggata
     3781 tatcggtaat gctttaaaag ataagcttat tgaacaagga catcaagtag atcaaattaa
     3841 tgttaggaat caattatgga agtcgacctc gttcaaagat tatgatgttt taattcatac
     3901 agcagctttg gttcacaaca attcacctca agcaaggcta tctgattata tgcaagtgaa
     3961 tatgttgctt acgaaacaat tggcacaaaa ggctaaagct gaagacgtta aacaatttat
     4021 ttttatgagt actatggcag tttatggaaa agaaggtcag gttggtaaat cagatcaaat
     4081 tgatacacaa acaccaatga accctacgac caactatggt atttccaaaa agttcgctga
     4141 acaagcatta caagagttga ttagtgattc gtttaaagta gcaattgtga gaccaccaat
     4201 gatttatggt gcacattgcc caggaaattt ccaacggtta atgcaattgt caaagcgact
     4261 gccaatcatt cccaatatta acaatcagcg cagtgcatta tatattaaac atctgacagc
     4321 atttattgat caattaatat cattagaagt gacaggcgtg tatcatcctc aagatagttt
     4381 ttactttgat acatcgtcag taatgtatga aatacgtcgc caatcacatc gtaaaacggt
     4441 attgatcaac atgccttcag tgttaaataa gtattttaat aagttgtcgg tctttagaaa
     4501 attattcggc aatttaacat acagcaatac gttatatgaa aataataatg cacttgaagt
     4561 tattcctgga aaaatgtcac ttgttattgc ggacatcatg gatgaaacga caaccaaaga
     4621 taaggcataa gtcatctatt aaataaaatc aacatacaaa tcgttttatt tggaggttat
     4681 agtatgaagt taacagtagt tggcttaggt tatattggtt taccaacatc aattatgttt
     4741 gcaaagcatg gcgtcgatgt gcttggtgtt gatattaatc agcaaacgat tgataagtta
     4801 caaagtggtc aaattagtat tgaagaacct gggttacaag aggtttatga agaggtactg
     4861 tcatcgggaa aattgaaggt atctacaacg ccagaagcat ctgatgtttt tatcattgcc
     4921 gttccgacgc cgaataatga tgatcagtac cggtcatgtg acatttcgct agttatgcgt
     4981 gcattagata gtattttacc atttttagaa aaagggaata ctattattgt agagtcgaca
     5041 attgcgccta aaacgatgga tgattttgta aaaccagtca ttgaaaattt aggatttaca
     5101 ataggtgaag atatttgttt agtgcattgt ccagaacgtg tactgccagg aaaaatttta
     5161 gaagaattag ttcataacaa tcgtatcatt ggcggtgtga ctaaagcttg tattgaagcg
     5221 ggtaaatatg tctatcgcac attcgttcag ggagaaatga ttgaaacaga tgcacgtact
     5281 gctgaaatga gtaagctaat ggaaaacaca tatagagacg tgaatattgc tttagctaat
     5341 gaattaacaa aaatttgcaa taacttaaat attaatgtat tagatgtgat tgaaatggca
     5401 aacaaacatc cgcgtgttaa tatccatcaa cctggtccag gtgtaggcgg tcattgttta
     5461 gctgttgatc cgtactttat tattgctaaa gaccctgaaa atgcaaagtt aattcaaact
     5521 ggacgtgaaa ttaataattc aatgccggcc tatgttgttg atacaacgaa gcaaatcatc
     5581 aaagcgttga gcgggaataa agtcacagta tttggtttaa cttataaagg tgatgttgat
     5641 gatataagag aatcgccagc atttgatatt tatgagctat taaatcaaga accagacata
     5701 gaagtatgtg cttatgatcc acatgttgaa ttagattttg tggaacatga tatgtcacat
     5761 gctgtcaaag acgcatcgct agtattgatt ttaagtgacc actcagaatt taaaaattta
     5821 tcggacagtc attttgataa aatgaagcat aaagtgattt ttgatacaaa aaatgttgtg
     5881 aaatcatcat ttgaagatgt atcgtattat aattatggca atatatttaa ttttatcgac
     5941 aaataaaatg tgtcaaacta gggcatacat gattaaggaa agataagctg tcatgtgttt
     6001 gaacttcaga gaggataatg ttatgaaaaa aattatggtt attttcggta cgagacccga
     6061 agcaataaaa atggcaccat tagtaaaaga aattgatcat aatgggaact ttgaagcgaa
     6121 cattgtgatt acagcacaac atagagatat gttagatagt gtgttaagta tatttgatat
     6181 tcaagctgat catgatttaa atattatgca agatcaacaa acgttagcgg accttacggc
     6241 gaatgcgctt gctaaacttg atagcatcat taatgaggaa cagccggata tgattttagt
     6301 acatggtgat actacaacga cttttgtagg aagtttggca gcattttatc atcaaattcc
     6361 ggttggacat gtagaagctg gacttcgaac acatcagaaa tactcaccat ttcctgaaga
     6421 gttaaatcga gtcatggtaa gtaatattgc cgaattgaat tttgcgccaa cagtaattgc
     6481 agctaaaaat ttactttttg aaaacaaaga caaagagcgt atctttatta ctggaaatac
     6541 agttattgac gcattgtcaa caacagttca aaatgatttt gtttcaacga ttattaataa
     6601 acataaaggc aagaaagtta ttttactaac agcgcatcgt cgtgaaaata ttggggaacc
     6661 gatgcatcag atttttaaag cagtaagaga tttggcagat gaatataaag atgttgtctt
     6721 catttatcca atgcatcgta atccaaaggt aagagcgatt gccgaaaaat atttatctgg
     6781 gagaaatcgg attgaattaa ttgagccatt agatgcgatt gagttccata attttacaaa
     6841 tcaatcgtac ctcgtgctga cagattctgg tggtattcaa gaggaggctc ctacatttgg
     6901 aaaacctgtg ttggtattaa ggaatcatac agagcgtccc gaaggcgttg aggcgggaac
     6961 atcgagagta attggcacag attatgacaa tattgttcga aatgtgaaac aattgattga
     7021 ggatgatgaa gcgtatcaac gtatgagtca agcgaataat ccatatggtg atggacaagc
     7081 atcacgacgt atttgtgaag caatagaata ttattttgga ttgcgctcag acaagccgga
     7141 tgaattcgta cctttacgtc acaaataata aaaaacccct aatcatgaag ttggtttaga
     7201 caaccagcgg tgactagggg tttttaatat atttattttt gatagtggta gccaatatca
     7261 tatttgaata ctttatttga taatattgga ctttgctgtc catcgtcatc actttttaaa
     7321 cgtacatttt tatgagcttc tttaaataca tcggaattca accaattatt aaagctatct
     7381 tcagattccc aaatagttaa gattttaact tcgtctgtat cctcggtatt taatgtttta
     7441 gtgacaaaca tttgttggaa gccttcaata gtttcaatgc cttgtctatt gtaaaaacgt
     7501 tcaatcgttt cttccgcact gcctttttgt aattgtaatc tattttctgc cataaacatg
     7561 ggcaatcact cctctatttt atgatttgat ttgggtaatg tttttacaaa tgtaaagagt
     7621 acagcggttt gtatgataac cattatgatt aatcctacac ggactgcaag aacatccacc
     7681 atataaattg aaaaacctat tacaatgtat aagctaatta aaattttaat tttctgttgt
     7741 agcgtgtagc ctcgatgtaa ataaaagttt tctacatatt ctttataaat tttttgatta
     7801 ataagccaat tgtaaaaccg atctgaactt cgagcaaaac aaaaaactgc tacgagtaaa
     7861 aaaggggtcg ttggcagtaa aggtaatacg gcacctgcaa taccaagcgc tgtaaatatt
     7921 aagccaataa cgattaaaat aagtcgcatt gaaaaaactc cattctagta ctaatgcgca
     7981 tgtaatattg ttttagtaat ataactcatg ctaaatataa tgtgtatgat aagtgcaatg
     8041 actcagtaaa atgaaacgtt gttgaattat tcttgtccca ttaacgcatt ttaagcgcga
     8101 ctttcataac aaccaaacta tttaatgaga attattctca agtattatag ttatattatg
     8161 tgttttattt ttgaaaagtg caatatgttt tcgaaaataa gattattttt atgtgcaaaa
     8221 acgacgcaaa agttttaaaa atgagacttc tgtgagctga ttattttata aaatgtaaac
     8281 gcttactata taatgtgaat catatcgttt aaaagcatta ttaaatatga tgctaagaga
     8341 tttatattat agccaataaa caaaggagag ataatatggc agtaaacgtt cgagattata
     8401 ttgcagagaa ttatggttta tttatcaatg gggaatttgt taaaggtagc agtgacgaaa
     8461 caatcgaagt gactaatcca gcaactggag aaacactatc acatattaca agagcaaaag
     8521 ataaagatgt cgatcatgca gtcaaagtgg cgcaagaggc atttgaatca tggtcattaa
     8581 cttctaaatc agaacgtgca caaatgttgc gtgatattgg tgataaatta atggcacaaa
     8641 aagataaaat tgcaatgatt gaaacattaa ataatggtaa accgattcgt gagacaacag
     8701 caattgatat tccatttgct gcaagacatt tccattattt cgcaagtgtt attgaaacag
     8761 aagaaggtac agtaaatgat atcgataaag acacaatgag tatcgtacga catgagccga
     8821 ttggcgtcgt aggtgctgtt gttgcttgga acttcccaat gctattagct gcatggaaga
     8881 ttgcgccagc cattgctgca ggtaatacaa ttgtgattca accttcgtct tcaacaccat
     8941 taagtttatt ggaagttgct aaaattttcc aagaggtatt acctaaaggt gttgtcaata
     9001 tactaacggg taaaggttca gaatcaggta atgcaatttt caatcatgat ggtgtagata
     9061 aattatcatt tacgggctca actgatgtag gttatcaagt tgccgaagct gcagcaaaac
     9121 atctagtacc cgctacatta gagcttggtg gtaaaagcgc caatatcata ttagatgatg
     9181 ctaatttaga ccttgcagtt gaaggtattc agttaggtat tttattcaac caaggtgaag
     9241 tatgtagtgc aggttctcga ttattagttc atgaaaaaat ttatgatcaa ttggtgccac
     9301 gtttacaaga ggcattttca aatattaaag ttggagatcc acaagatgaa gctacacaaa
     9361 tgggtagtca aactggtaag gatcaattag ataaaattca atcatatatt gatgcagcaa
     9421 aagaatcaga tgcacaaatt ttagcaggtg gtcatcgctt aactgaaaat ggattagata
     9481 aagggttctt ctttgagccg acattaattg ctgtgccaga caatcatcac aaattagcac
     9541 aagaagaaat atttggacca gtgttaacag tgattaaagt gaaggacgat caagaagcaa
     9601 ttgatatagc taatgattct gagtatggtt tagcaggcgg tgtattttct caaaatatca
     9661 cacgtgcatt aaatattgct aaagctgtac gtacaggacg tatttggatt aacacttaca
     9721 accaagtacc agaaggcgca ccatttggtg gttataaaaa atcaggtatc ggtcgagaaa
     9781 cttataaagg tgcgttaagt aactatcaac aagttaaaaa tatttatatt gatacaagca
     9841 atgctttaaa aggtttgtac tagaataaat atcgtttctg aagcgtgttt gtaggtcagt
     9901 ctagcggtaa gtcttaacat ttaacggcgt tgtttagatt ttaagcaaaa caaaatatat
     9961 aggaacacgt atcatgatat taggatataa tgactaaaat aatagcagta ggatggtttt
    10021 taattgcaaa tcatcttact gctgttttta attatgctaa tttgcgatgc agctattata
    10081 aggacagagt tgtttatgaa ttgtggtgat ttagaaatat gaagttcaat atgcaaagtc
    10141 atcgtttgtt ttaatatgtg gaacaatcat taaagttatt gtgatttttt gaacttaatg
    10201 aaactaaaca ataaatttga gatacttttt tgtcattttt atgtaactaa cacaataatc
    10261 tcgtacatta ttaaaatttt ctatatgata gggataaagt aaagagcgaa tgtgctgtaa
    10321 aagtttttcc aaggtgatat tacataaagg tataaagggt aaagattaat tagttgtcat
    10381 gtaaaggacg atgatgtata aatcatggtt aattacggaa gcattaatat taatctgaga
    10441 agctataaag aattattttt aaaagcgaca atattaaata cgacgcattt atttaggagt
    10501 ggcaaacgta tgaatgggaa aaaggcaaat acgataaaca gatacaaata ttttcatcat
    10561 gtcaatcatc aaaaaattca acaaagttct aaaaagacgc tgtgggcatc actaatcatc
    10621 acattgttat ttacagtgat tgaatttgtc ggaggtttag tatctaattc attggcatta
    10681 ctgtcagatt catttcatat gcttagtgat gtattagcac ttggtttatc tatgttggcc
    10741 atttattttg caagtaaaaa gccgactgca cgatacacat ttggatattt aagatttgag
    10801 atattagttg catttttaaa tggtttagca ttaattgtaa tttcaatctg gattttatat
    10861 gaagctattg tacgtattat ttatccgcaa ccaattgaaa gtggcattat gtttatgatt
    10921 gctagtattg gtttactcgt taatattatt ttgacaatta tccttgtaag gtctttaaaa
    10981 caagaagaca atatcaatat tcaaagtgca ttatggcatt tcatgggaga cttattgaac
    11041 tctattggtg tcatcgttgc agttgtattg atttacttta caggatggcg catcatcgac
    11101 ccaatcatta gtattgtaat ttcactcatc attttacgtg gtggttataa aattacgcgt
    11161 aatgcgtggt taattttaat ggaaagtgtg cctcaacatt tggatactga tcaaattatg
    11221 gcagatatta aaaacataga tggcatatta gatgtacatg aatttcattt gtggagtatt
    11281 acaacagagc attattcatt aagtgcccat gttgtgttag ataaaaaata tgagggtgat
    11341 gattatcaag cgattgatca agtatcatca ttgttgaaag aaaaatatgg cattgcacat
    11401 tcaacgttgc aaattgaaaa cttgcaattg aatccattag atgagccata cttcgacaaa
    11461 ttaacataaa taaaacattg tacaggctgg gacataaatc aatgttctat gctctacgaa
    11521 gttatattgg cagtagttga ctgaacgaaa atgcgcttgt aacaagcttt tttcaattct
    11581 agtcaggggc cccaacacag agaatttcga aaagaaattc tacaggcaat gcaagttggg
    11641 gtgtgggccc caacaaagag aaattggatt cccaatttct acagacaatg caagttgggg
    11701 tgggacaacg aaataaattt tgcgaaaata tcatttctgt cccactccca cgtttgtttt
    11761 ggattatgtt gcatcattta aatgattttc gtcaatttct ttgatgctat ctacatctaa
    11821 cacgacatct ttaggtttca aaatatgaat atgtttttca tcatttgtat gtaaaatgcg
    11881 ttctatgatg tatctttgac cggccattgt ttctacagca atctttttgt ttctagctaa
    11941 acttgctacg acagattctt tatccataat gatagccccc tatatatatg tttatttact
    12001 tataccctaa catgattttt ata
//
LOCUS       DANBWH010000071        12014 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.074, whole genome shotgun sequence.
ACCESSION   DANBWH010000071 DANBWH010000000
VERSION     DANBWH010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12014
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.074"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            94..273
                     /gene="prli42"
                     /locus_tag="PPY92_001335"
     CDS             94..273
                     /gene="prli42"
                     /locus_tag="PPY92_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stressosome-associated protein Prli42"
                     /protein_id="HDJ2625347.1"
                     /translation="MLVISSQVKSFISYVVIINTKVIHLEAHKMQNKVLRIIIIVMLV
                     SVVLALLLTSIIPIL"
     gene            complement(400..1380)
                     /locus_tag="PPY92_001336"
     CDS             complement(400..1380)
                     /locus_tag="PPY92_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDJ2625348.1"
                     /translation="MLKLNPYKIGFRTIKTAVGMTLGVIISKLLGLDNYASSAILVVL
                     CIKHTKVHSLQAIISRLVSCFLVLFLGSAIFSLLGQSPIVLGIIVLLFIPLTVVLKVQ
                     EGVITSCVILLHVFNAKSIDAHLIVNETLLLLIGLSIAFTMNLMMPSLDKQLDEYKCK
                     IEQQIADIFSKYSYICEKYEDTIAIEFEVLLLNIKKAKSIAFRDVKNHFVRNENSYYH
                     YFDMREEQVELLMRMKPLIESICHKDPLLNDVSSLLKEIGLNVNSNDYTAMRLHNLYE
                     TRLKLDELPLPTTHKALNSRASIIQILNELEEYLQIKSQFGSLKMHSEVA"
     gene            complement(1394..1831)
                     /locus_tag="PPY92_001337"
     CDS             complement(1394..1831)
                     /locus_tag="PPY92_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HDJ2625349.1"
                     /translation="MDMNFDLYMNGVVEQARNEIESAGYEQLTTAEDVDKVLKQDGTT
                     LVMINSVCGCAGGIARPAASHALHYDVLPDRLVTVFAGQDKEATQRAREYFEGYAPSS
                     PSFALVKDGKITEMIERHQIEGHDVMNVINQLQTLFNKYCEER"
     gene            complement(2182..3456)
                     /locus_tag="PPY92_001338"
     CDS             complement(2182..3456)
                     /locus_tag="PPY92_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDJ2625350.1"
                     /translation="MEITMPKLGESVHEGTIEQWLVSVGDHIDEYEPLCEVITDKVTA
                     EVPSTISGTITEILVEAGQTVAIDTIICKIETADEKTNETTEEIQAKVDEHTQKSTKK
                     ASATVEQTFTAKQNQPRNNGRFSPVVFKLASEHDIDLSQVVGSGFEGRVTKKDIMSVI
                     ENGGTTAQSDKQVQTKSTSVDTSSNQSSEDNSENSTIPVNGVRKAIAQNMVNSVTEIP
                     HAWMMIEVDATNLVNTRNHYKNSFKNKEGYNLTFFAFFVKAVADALKAYPLLNSSWQG
                     NEIVLHKDINISIAVADENKLYVPVIKHADEKSIKGIAREINTLATKARNKQLTTEDM
                     QGGTFTVNNTGTFGSVSSMGIINHPQAAILQVESIVKKPVVINDMIAIRSMVNLCISI
                     DHRILDGLQTGKFMNHIKQRIEQYTLENTNIY"
     gene            complement(3469..4452)
                     /locus_tag="PPY92_001339"
     CDS             complement(3469..4452)
                     /locus_tag="PPY92_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDJ2625351.1"
                     /translation="MAKLSYLEAIRQAQDLALQQNKDVFILGEDVGKKGGVFGTTQGL
                     QQQYGEDRVIDTPLAESNIVGTAIGAAMVGKRPIAEIQFADFILPATNQIISEAAKMR
                     YRSNNDWQCPLTIRAPFGGGVHGGLYHSQSIESIFASSPGLTIVIPSTPYDAKGLLLS
                     SIESNDPVLYFEHKKAYRFLKEEVPEEYYTVPLGKADVKREGEDLTVFCYGLMVNYCL
                     QAADILAADGINVEVVDLRTVYPLDKETIIDRAKNTGKVLLVTEDNLEGSIMSEVSAI
                     IAEHCLFDLDAPIMRLAAPDVPSMPFSPVLENEIMMNPEKILNKMRELAEF"
     gene            complement(4452..5444)
                     /locus_tag="PPY92_001340"
     CDS             complement(4452..5444)
                     /locus_tag="PPY92_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HDJ2625352.1"
                     /translation="MIDYKSLGLSEEDLKVIYKWMDLGRKIDERLWLLNRAGKIPFVV
                     SGQGQEATQIGMAYALEEGDITAPYYRDLAFVTYMGISAYDTFLSAFGKKDDVNSGGK
                     QMPSHFSSRAKNILSQSSPVATQIPHAVGAALALKMDGKKKIATATVGEGSSNQGDFH
                     EGLNFAGVHKLPFVCVIINNKYAISVPDSLQYAAEKLSDRALGYGIHGEQVDGNDPLA
                     MYKAMKEARDRAISGQGSTLIEAVTSRMTAHSSDDDDQYRTKEEREALKKADCNEKFK
                     KELLSAGIIDDAWLAEIEAEHKDIINKATKAAEDAPYPSVEEAYAFVYEEGSLN"
     gene            complement(5460..6881)
                     /gene="lpdA"
                     /locus_tag="PPY92_001341"
     CDS             complement(5460..6881)
                     /gene="lpdA"
                     /locus_tag="PPY92_001341"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDJ2625353.1"
                     /translation="MSEKQYDLVVLGGGTAGYVAAIRASQLGKKVAIVERQLLGGTCL
                     HKGCIPTKSLLKSAEVFQTVKQAAMFGVDVKDANVNFENMLARKEDIINQMYQGVKHL
                     MQHNHIDIYNGTGRILGTSIFSPQSGTISVEYEDGESDLLPNQFVLIATGSSPAELPF
                     LSFDHDKILSSDDILSLKTLPSSIGIIGGGVIGMEFASLMIDLGVDVTVIEAGERILP
                     TESKQASQLLKKSLSARGVKFYEGIKLSENDINVNEDGVTFEISSDIIKVDKVLLSIG
                     RKPNTSDIGLNNTKIKLSTSGHILTNEFQQTEDKHIYAAGDCIGKLQLAHVGSKEGVV
                     AVDHMFEGNPIPVNYNMMPKCIYSQPEIASIGLNIEQAKAEGMKVKSFKVPFKAIGKA
                     VIDSHDANEGYSEMVIDQSTEEIVGINMIGPHVTELINEASLLQFMNGSALELGLTTH
                     AHPSISEVLMELGLKAESRAIHV"
     gene            complement(7032..8711)
                     /gene="recN"
                     /locus_tag="PPY92_001342"
     CDS             complement(7032..8711)
                     /gene="recN"
                     /locus_tag="PPY92_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDJ2625354.1"
                     /translation="MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQ
                     LIGMRASSDFVRHGEKKAVIEGIFDIDESKDAIHILKNMDIDVDEDFLLVKREIFSSG
                     KSLCKINNQTVTLQDLRKVMQELLDIHGQHETQSLLKQKYHLTLLDNYAESRYQDLLD
                     KYHQTFQNYKAKKQELEDLESADQALLQRLDLMKFQLEELSEAHLKEGEIEQLEIDIK
                     RIQNSEKLSLALNNAHMTLTDENAITDRLYELSNHLLTINDIVPNKYDKLKEDIDQFY
                     YILEDAKHELYDEMANTEFDEQVLNEYESRMNLLNNLKRKYGKDISELIAYQEKLNNE
                     INKIENYEQSTSQLREEINALYNQVIEVGQALSKQRCIVARELRDHIVSEIQNLQMKD
                     ANLEISFKKLEEPNIDGIEFVEFLISPNKGEPLKSLNKIASGGELSRIMLALKSIFVK
                     SRGQTAILFDEVDSGVSGQAAQKMAEKMRDIAEYIQVICISHLPQVASMSDHHLLISK
                     SSKDDRTTTQVQELIGDDKVDEIARMISGASVTDLTRENAREMIQHNQRRR"
     gene            complement(8727..9179)
                     /gene="argR"
                     /locus_tag="PPY92_001343"
     CDS             complement(8727..9179)
                     /gene="argR"
                     /locus_tag="PPY92_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDJ2625355.1"
                     /translation="MPKKSVRHIKIREIISNEQIETQDELVKRLNDYDLNVTQATVSR
                     DIKELQLIKVPIPSGQYVYSLPNDRKFHPLEKLGRYLMDSFVNIDGTDNLLVLKTLPG
                     NAQSIGAILDQINWEEVLGTICGDDTCLIICRSKEASDEIKSRIFNLL"
     gene            complement(9611..10492)
                     /locus_tag="PPY92_001344"
     CDS             complement(9611..10492)
                     /locus_tag="PPY92_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDJ2625356.1"
                     /translation="MTNLPMNKLIDEVNNELSVAINKSVMDTQLEESMLYSLNAGGKR
                     IRPVLLLLTLDSLNTEYELGVKSAIALEMIHTYSLIHDDLPAMDNDDYRRGKLTNHKV
                     YGEWTAILAGDALLTKAFELISSDDRLTDEVKIKVLQRLSIASGHVGMVGGQMLDMQS
                     EGQPIDLETLEMIHKTKTGALLTFAVMSAADIANVDDATKEHLESYSYHLGMMFQIKD
                     DLLDCYGDEAKLGKKVGSDLENNKSTYVSLLGKDGAEDKLTYHRDAAVDELTQIDEQF
                     NTKHLLEIVDLFYSRDH"
     gene            complement(10470..10700)
                     /locus_tag="PPY92_001345"
     CDS             complement(10470..10700)
                     /locus_tag="PPY92_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDJ2625357.1"
                     /translation="MTKETQSFEEMMQELEQIVQKLDNETVSLEESLDLYQRGMKLSA
                     ACDTTLKNAEKKVNDLIKEEAEDVKNDESTDE"
     gene            complement(10693..>12014)
                     /locus_tag="PPY92_001346"
     CDS             complement(10693..>12014)
                     /locus_tag="PPY92_001346"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDJ2625358.1"
                     /translation="VSALTKYIKYKFDQDPHLQSVLIKGELSNFKKHSSGHLYFNVKD
                     KESVISAMMFKGSASKLNFEPKEGDEVLLEARVSVFERRGNYQIYVNKMQLDGIGNLY
                     QKLEALKKKLTEEGCFDKANKKSIPKFPKKIAVLTASTGAAIRDIHSTINSRFPLAEQ
                     IQISTLVQGEKAKDDIIEKIEYADSLGVDTIIVGRGGGSIEDLWNFNEEAVVRAIYNC
                     KTPIISAVGHETDFTLSDFAADIRAATPTQAAVIATPDQYELLQQIQQYQFTLTRFIK
                     KHLEQQRKHVEHLSSYYKFKQPTLLYDQQIQRRDDLEKRLKQQIQATFEQQRHRLMLL
                     QQRYNLKALLSSVNQEQQNNLQLTNQLVKLLNSKILSYKNDLKNKVENLNNLSPTNTM
                     LRGYAIVNKKDEVITSTKDLTENDQLTLTMKDGLVDAKVTKVRCNND"
BASE COUNT         3633 a         2263 c         1631 g         4487 t
ORIGIN      
        1 aatatattga ttgatttttc aaagatatcg tataatgagg aaaatgacat aagcaaactt
       61 actcatgttt tttattatat tccttaatga tgattgctag ttatatcgtc tcaagttaaa
      121 agttttatat cttatgtcgt aattattaat acaaaggtta ttcatttgga ggcacacaaa
      181 atgcaaaata aagttttaag aattatcatt atcgttatgc ttgtatcagt tgtattagca
      241 ttgttattaa cgagtatcat tccaatttta taaactatat ctcaactacc tatacaaaat
      301 catacaatta aaaatccatc cgttataaac gcatgtatta ataagttatc gtattgcaac
      361 gattactttc aaacatgggt catacggatg gattattttt taagctactt cactatgcat
      421 tttcaatgaa ccaaattgcg atttgatttg taaatattct tctaattcat ttaatatttg
      481 aataatactt gctctcgagt taagcgcttt gtgtgttgtt ggcaatggca gttcatccaa
      541 tttcaaacgc gtctcataca aattgtgtaa acgcattgct gtatagtcat tactattcac
      601 atttagacca atttctttca gcagtgacga aacatcattt aaaagtggat ctttatgaca
      661 gatactttcg atgagcggtt tcattctcat taacaattcc acttgctctt ctcgcatatc
      721 aaaataatga tagtatgaat tttcgtttct aacaaaatga tttttaacat ctcggaacgc
      781 gatagacttc gcctttttaa tatttaaaag taacacttca aattcaatcg caatggtatc
      841 ttcatatttt tcacaaatat aactatattt actaaaaata tcagcaattt gttgctcaat
      901 tttacatttg tattcgtcta gttgtttgtc taaacttggc atcattaaat tcattgtaaa
      961 tgcaatgctc agtccaatta acagtaataa tgtttcatta acaattaaat gtgcatcaat
     1021 tgattttgca ttaaaaacat gaagtaatat aacgcaactc gtaatgacac cttcttgtac
     1081 ttttaatacg acagttaatg gtataaataa caatacgata ataccgagta caattggact
     1141 ctgacctaat aaactaaata ttgctgaacc taaaaacaat actaaaaaac atgatactaa
     1201 tcttgaaata atcgcttgta gcgaatgtac ttttgtatgt ttaatacata atacgactaa
     1261 tatggcgctt gaagcataat tatctaaacc taacagctta ctaataatta cacctaaagt
     1321 catacccact gctgttttta ttgttctaaa tccaatcttg taaggattta actttaacat
     1381 gggttagcgc ctcttatctt tcttcacaat atttattgaa taatgtttgt aattgattaa
     1441 ttacgttcat cacatcatga ccttcgattt gatgtctttc aatcatttct gtaatctttc
     1501 catcttttac taatgcaaat gacggacttg aaggcgcata accttcgaag tattcacgcg
     1561 ctctttgtgt cgcttcttta tcttgtccag caaatactgt cactagacga tcaggtaata
     1621 cgtcataatg taaagcatgt gatgctgctg gtcttgcgat accacctgca caaccacata
     1681 cagaattgat cataactagt gttgtaccat cttgtttaag aactttgtca acatcttctg
     1741 cagtagttaa ttgctcatat cccgcagatt caatttcatt ccttgcttgt tctacaacac
     1801 cgttcatgta taaatcgaaa ttcatgtcca taagttcaat cacctatccc tttatattta
     1861 aactatcctc attctactaa ttaataacat attgttcaat aaactaatct gaatcacacc
     1921 aatatttaga cacaatttta acaatatacc aaacattatt gtgcttaata tcatggaaac
     1981 taatttgttc acctgttttc attaatatgt ttccagtatg atgtattatt ttgactttac
     2041 tgcaaaaatg cattcaacca tgttgattat tgttctttat ctttttttga atttattgca
     2101 catattttat tgcccaaaaa aatacatcca tcgacaagaa caagataaaa caagttgtcg
     2161 atagatgtat ccatgttatc actaatatat atttgtattt tctaaagtat actgttcgat
     2221 acgctgttta atatgattca taaatttacc agtttgtaaa ccatctaaaa tgcgatgatc
     2281 tattgaaata cataaattta ccatacttcg aattgcaatc atatcattaa ttactactgg
     2341 ctttttaacg attgattcta cttgtaatat cgctgcttga gggtgattta taatccccat
     2401 tgatgatact gaaccaaatg taccagtatt attaactgta aatgttccac cttgcatatc
     2461 ttcagttgtc aattgcttat tacgcgcttt tgttgctaaa gtattaattt ctctagctat
     2521 acctttgatt gacttttcgt ctgcatgctt aatcacaggt acgtataatt tattttcatc
     2581 agcaacagca attgaaatat taatgtcttt atgtaagaca atttcatttc cttgccagct
     2641 actatttaat aaaggatatg cttttaaagc atctgctaca gcttttacaa agaaagcaaa
     2701 gaacgttaga ttatatcctt ctttattttt aaagctgttt ttataatgat ttctcgtatt
     2761 cacaagattt gtagcatcta cttcaatcat catccatgca tgtggaatct ctgttacact
     2821 attaaccata ttttgcgcaa ttgctttacg cacaccattt actggtattg tgctgttttc
     2881 actattgtct tcagatgatt ggttacttga tgtatctact gatgttgatt ttgtttgaac
     2941 ttgtttgtca gattgagctg tggtaccacc attttcaata actgacatta tatccttctt
     3001 agttacacga ccttcaaatc cactacctac aacttgtgat aaatcaatgt catgctctga
     3061 agcgagttta aatacaacag gtgaaaagcg accattatta cgaggttgat tttgtttagc
     3121 agtaaatgtc tgttccactg ttgcactagc ttttttagta gatttctgag tatgctcatc
     3181 cacttttgct tgtatctctt cagttgtttc atttgtcttt tcatcagcag tttcaatttt
     3241 acagataatt gtatcaatag ctactgtctg ccccgcttca actaaaattt ctgtaattgt
     3301 tcctgatatc gtggaaggga cttcagctgt cactttatct gtaataactt cacataatgg
     3361 ttcatattca tcaatatgat caccaacaga aactaaccat tgttcaatgg taccttcatg
     3421 aacactctca cctaacttag gcattgttat ttccatgact ttccctccct agaattctgc
     3481 taattcacgc attttattta agattttttc tggattcatc ataatttcat tttctaatac
     3541 aggagaaaat ggcatagatg gtacatctgg agcagctaaa cgcatgattg gtgcatctaa
     3601 atcgaacaag caatgctctg caataatcgc tgacacttct gacataatac taccttctaa
     3661 attatcttca gttacaagta aaactttacc tgtattttta gcacgatcaa taattgtttc
     3721 tttatctaat ggataaacag ttcgtaaatc aacgacttca acgttgatac cgtctgcagc
     3781 taaaatatcc gctgcttgta aacaataatt gaccattaat ccataacaaa atactgttaa
     3841 atcttcacct tcacgtttaa catctgcttt tcctaaaggt acagtgtaat attcttctgg
     3901 cacttcttcc tttaagaaac gataagcttt tttatgctca aagtacaata ctggatcatt
     3961 tgattcgata gatgataata aaagcccttt agcatcatac ggtgtggaag gaataacaat
     4021 tgttaaacct ggtgatgaag caaatatact ttcaatactt tgtgaatgat atagtcctcc
     4081 gtgaacaccg ccaccaaatg gtgcacgaat cgttaatggg cattgccaat cattatttga
     4141 acgataacgc attttcgcag cttcactaat aatttgattt gtcgcaggta aaataaaatc
     4201 tgcaaattga atttctgcaa ttggtctttt acctaccata gctgcaccaa tggcagttcc
     4261 aacaatattt gactcagcta atggcgtatc gataactctg tcttcaccat attgttgttg
     4321 tagtccttga gtagtaccaa atacgccacc ttttttacca acatcttcac caagaataaa
     4381 cacatcttta ttttgttgta atgctaagtc ttgtgcctgg cgtatcgcct ctaaataaga
     4441 taatttagcc attagttaag actcccttct tcgtacacaa atgcataggc ttcttcgaca
     4501 cttggatatg gcgcgtcttc agcagccttt gtcgctttat tgatgatgtc tttatgctcc
     4561 gcttctattt ctgccaacca agcatcatcg ataatgccag ctgaaagcaa ctcttttttg
     4621 aacttttcat tgcagtcagc ttttttaagc gcttcacgct cttctttcgt acgatattgg
     4681 tcgtcatcat ctgatgaatg agctgtcata cgacttgtta ctgcttcaat caaagttgaa
     4741 ccttgaccag aaatagctcg atctcttgct tctttcatcg ctttatacat tgctaatgga
     4801 tcattaccat ctacttgttc accatgtata ccgtaaccaa gtgctctatc cgataatttt
     4861 tcagctgcgt attgtaatga atcaggtact gaaattgcat atttattatt tataatgaca
     4921 catacaaaag gaagtttgtg tacacccgcg aagtttaaac cttcatggaa gtcaccttgg
     4981 tttgagctac cttcaccaac agttgctgtt gcaattttct tcttaccatc catttttaaa
     5041 gctaaagcag caccaacagc atggggtatt tgagttgcta ccggtgaact ttgagacaaa
     5101 atattcttag ctctactact aaagtgtgat ggcatttgtt ttccaccaga gttaacatcg
     5161 tctttctttc caaacgctga taaaaacgta tcatacgctg agatacccat ataagtaacg
     5221 aaagctagat ctctataata aggcgctgta atatcacctt cttctaatgc gtatgccatc
     5281 ccaatctgag ttgcttcttg tccttgacca cttacaacaa atggaatttt acctgcacgg
     5341 ttcaataacc acagtctttc atctattttt ctacctaaat ccatccattt atatattact
     5401 tttaggtctt cttcgctaag gcctaatgat ttataatcaa tcatgttaaa tcctcctatt
     5461 tatacgtgaa tagctctact ttctgctttc aatcctaatt ccatcaacac ttcagagatg
     5521 gaaggatgtg cgtgtgttgt tagtcctaat tctaatgccg agccattcat gaactgtaac
     5581 agtgatgcct cattaatcaa ttctgttaca tgtggaccaa tcatattaat acccacaatt
     5641 tcttcagttg attgatcaat caccatttcg ctataccctt cgtttgcgtc atggctatca
     5701 atcactgctt taccaattgc tttaaatggt actttaaaac ttttaacttt cattccctct
     5761 gcctttgctt gttcaatgtt taaaccgata gaagcaattt caggttgtga ataaatacac
     5821 ttaggcatca tgttatagtt tactgggatt gggttcccct caaacatatg atcaacagcc
     5881 acaacacctt cttttgatcc aacatgtgcc aattgtaatt ttcctataca atcaccagct
     5941 gcataaatat gtttatcttc agtttgttga aattcgttcg ttaaaatatg tcctgatgta
     6001 gaaagtttta ttttagtgtt gtttaaacca atatctgatg tgttaggttt tctaccaatc
     6061 gatagcaaca ctttatctac tttaattatg tctgaagaaa tttcaaacgt aacaccatct
     6121 tcgttaacat ttatatcatt ttcagaaagt tttattccct catagaattt aacaccacgt
     6181 gctgacaatg atttttttaa tagttgtgaa gcttgtttac tttcagttgg taaaattctt
     6241 tcacctgctt ctataactgt tacgtcaaca cctaaatcta tcatcaatga tgcaaattcc
     6301 attccgataa caccaccacc aataatacca atacttgatg gtaacgtctt taatgataat
     6361 atatcatcgc tagataaaat tttatcatga tcaaatgata agaatggcaa ctctgcaggc
     6421 gaagaaccag ttgcaattaa tacaaattgg ttgggtaata agtctgattc accatcttca
     6481 tattcgacag aaattgtgcc actttgaggt gaaaatatag atgtacctag aatacgtccc
     6541 gtgccattat aaatgtcaat gtgattgtgt tgcattaaat gctttacacc ttgatacatt
     6601 tgattaataa tgtcttcttt tcgtgccaac atattttcaa aattaacatt agcatctttg
     6661 acatcaacgc caaacattgc tgcctgtttt actgtttgaa atacttcagc agatttaagc
     6721 agcgatttag taggaataca acctttatgg agacaagtac ctcctaatag ttgtcgttct
     6781 actattgcca ctttcttacc taattgagac gcacgtatcg cagcaacata tcctgcagta
     6841 cctccaccga gaacgactaa atcatattgt ttctctgaca tgttcttact cctaactaat
     6901 gatatatatc cattgaaaat ttattaatac atagttttca tgtccattaa ttacctattt
     6961 tacatgattg tctatttagt ttgaatgcac ataaataaat ccataaatga gtattcaaca
     7021 cactttggaa attatctacg tctttgattg tgttgtatca tttctcttgc attttctcga
     7081 gttaaatcag taacacttgc acctgaaatc attcgtgcaa tttcatctac tttatcatcg
     7141 ccaattaact cttgaacttg tgttgttgta cgatcatctt ttgatgactt cgaaattaat
     7201 aaatgatggt cgctcatcga tgcaacttgt ggcaagtgag aaatacaaat aacttgtata
     7261 tattctgcta tatctcgcat tttctctgcc attttttgtg cggcttgtcc agatacacct
     7321 gagtcaacct catcgaataa aattgcagtt tgacctctcg atttaacaaa aatacttttt
     7381 aacgctaaca taattctaga aagttctcca cctgatgcaa ttttatttaa actttttaat
     7441 ggttcccctt tatttggact gattaaaaat tctacaaatt cgattccatc aatattcggt
     7501 tcttctaatt ttttaaatga aatctcaaga tttgcgtctt tcatttgtaa gttttgaatt
     7561 tccgatacaa tgtggtctct taactcccta gcgactatac aacgctgctt tgataacgct
     7621 tgtccaacct ctataacttg attatacaat gcatttattt cttctcgtaa ctgcgatgta
     7681 ctttgttcat agttttcaat tttattgatt tcattattaa gcttttcttg atatgcgatt
     7741 aattctgaaa tatcttttcc atatttacgt tttaaattat tcagcaaatt catacgagat
     7801 tcatactcgt ttaacacttg ttcgtcgaat tctgtattag ccatttcatc atataactca
     7861 tgttttgcat cttctaaaat gtaataaaat tgatcaatat cttcttttaa tttgtcatat
     7921 ttatttggaa ctatatcgtt tattgttaac aaatggttgc ttagttcata caaacgatca
     7981 gtgatagcat tttcatccgt taatgtcata tgtgcgttat taagcgctaa gcttaatttt
     8041 tcagagtttt gaatgcgttt aatatctatt tcaagttgct ctatttcgcc ttcttttaga
     8101 tgtgcttcag acaattcttc taattggaat ttcattaaat ctaaacgctg cagcaatgct
     8161 tggtctgctg attctaaatc ttctaactct tgctttttgg ctttataatt ttgaaaagtt
     8221 tggtgatatt tatccaacaa atcttgataa cgtgattctg cgtaattatc caataatgtt
     8281 aaatggtatt tttgtttcaa caaagactgc gtttcatgtt ggccatgaat atctaataat
     8341 tcttgcataa cttttcgtaa atcttgtaaa gtaactgttt gattattaat tttacaaaga
     8401 cttttaccag agctaaaaat ttcccgttta actaataaaa aatcttcatc tacatcaata
     8461 tccatatttt tcaatatatg tatagcatct ttactctcgt caatatcaaa tataccttcg
     8521 atgacagcct ttttttcacc atgtcttaca aaatcagatg aagctctcat tccaattaat
     8581 tgtccaattg catctataat aattgactta cctgaacccg tttcaccact taaaacagtt
     8641 aaaccatcag aaaattgaat ttctaattct tcaataatag caaattgctt gattgataag
     8701 gtttgtaaca taaactcatc gcatccttat aacaaattga aaattcttga cttgatttca
     8761 tcacttgcct ctttgcttcg acaaataatt aaacaagtat catcaccaca aattgtgcct
     8821 agtacttctt cccaattgat ttggtctaat atagctccaa tagattgtgc attaccaggt
     8881 aatgttttta gaacaagtaa attatcagta ccatctatat taacaaagga atccattaaa
     8941 taacgtccca atttttctaa aggatggaat tttctatcat ttggtaaact ataaacatat
     9001 tgacctgaag gtataggtac tttaataagt tgtagttctt taatatcacg agaaacagtt
     9061 gcttgagtga catttaaatc ataatcgttt aatcgtttaa ctaattcatc ttgtgtctct
     9121 atctgttcat ttgaaataat ttctctaatt tttatatgcc taaccgattt tttgggcaca
     9181 gtaaacacct ctgattacga atatttatac atttatttta acacatgcac tgatttacga
     9241 ctactaaaca cctttacgta aaaagggtaa acatggttta tctatcttgg ttatctattt
     9301 ataaatattt ttcatattac gcataacaat tgcttaaaat atgtataaaa atgaatatat
     9361 gtgtaataaa cttgctaatt attagattta ataagcgtca attgtttgaa catatttaat
     9421 taaaatcaca ttgatgtcac agatacgaat attgtcgtat agaaattgaa aattctattt
     9481 tttaaatgaa agtcttcaac ataattttaa gtttcaacat gagaaaaatc gattaacaaa
     9541 caacgtcagt tgaatatgcc ttttgagaca tttcaaactt tacaattgtt gctaatcgat
     9601 atatttgctt ttagtgatcc ctgctataaa ataaatcaac gatttctaat aagtgttttg
     9661 tattgaattg ttcatcaatt tgcgttagtt catccactgc tgcgtctcta tgataagtca
     9721 atttatcttc tgcgccatct ttccctaata aactcacgta cgtactttta ttattttcaa
     9781 gatcgctgcc cactttttta cctaactttg cttcatcacc atagcagtct aataaatcat
     9841 ctttaatctg gaacatcata cctaaatgat aactataact ttctaaatgt tctttagttg
     9901 catcatcgac attagcgata tctgctgcac tcataaccgc aaaagttaat aatgctcctg
     9961 tttttgtttt gtgtatcatt tccaaagttt caagatcaat tggttggcct tcgctttgca
    10021 tatctaacat ttgaccgccg accattccaa catgaccact tgctattgac agccgttgta
    10081 gaacttttat ttttacttca tcagttaatc tatcatcact tgaaataagt tcaaatgctt
    10141 tagttaataa agcatcacct gctaatatcg cagtccactc accatatact ttatgatttg
    10201 ttaatttccc tcgtcgataa tcatcattat ccatcgctgg taggtcatca tgaataagtg
    10261 aatatgtatg aatcatttct agtgcaattg cgctcttcac acctaactca tactcggtat
    10321 ttagtgaatc taaagtgagt aataacagaa ctggtcggat gcgtttacct ccagcattta
    10381 atgaatacaa catgctttct tctagctgag tatccattac tgatttattt atcgcaaccg
    10441 ataattcatt attgacttca tctattaatt tattcatcgg tagattcgtc attttttaca
    10501 tcctcagctt cttcttttat taagtcattc accttttttt cggcattttt taaagttgtg
    10561 tcacaagctg ctgatagttt cataccacgt tgatataaat ctaatgattc ctctaaagat
    10621 actgtttcat tatctaattt ttgaacaatt tgctctaatt cttgcatcat ttcttcaaaa
    10681 ctttgcgttt ctttagtcat tattacacct tactttcgta acttttgcat ctactaagcc
    10741 atctttcatt gttaacgtca attgatcatt ttctgttaaa tctttagtac tcgtaatgac
    10801 ttcgtctttt ttattaacaa ttgcatatcc acgcaacatt gtattagttg gacttaaatt
    10861 gtttaagttt tctactttat ttttcaaatc atttttataa cttaatatct tagaattcaa
    10921 taatttaaca agttggtttg tcaattgaag attattttgt tgttcttgat taacactact
    10981 tagtaatgct tttaaattat aacgttgttg caacagcatt aatcgatgtc tctgttgttc
    11041 aaaagttgcc tgaatttgtt gtttcagtct cttttctaaa tcatctcgac gttgtatctg
    11101 ttgatcatac aataaagttg gttgtttaaa cttgtaatac gatgacaaat gttcaacatg
    11161 tttacgttgt tgttctaaat gtttcttgat gaaacgagtc aatgtaaact gatattgctg
    11221 tatttgttgc agcaattcat attggtctgg tgttgcaata acagcagctt gagttggagt
    11281 cgcagctctg atgtctgcag caaaatcact taatgtaaag tctgtttcat gacctactgc
    11341 tgatataatc ggtgtcttac aattatatat tgcacggacg acagcttctt cgttgaaatt
    11401 ccataaatct tctatggatc caccacctcg acctacaata atggtatcta cacctaaact
    11461 atctgcatat tcaatttttt caataatgtc gtcttttgct ttttcacctt gaaccaaagt
    11521 actaatttgt atttgttcag ctaatggaaa acgactattt atcgttgaat ggatatctcg
    11581 aattgcggca cctgtactcg ctgttaaaac tgcaattttt ttaggaaact taggtattga
    11641 tttcttattc gctttatcaa aacaaccttc ttcagttaat tttttcttta atgcttctaa
    11701 tttttgatat aagttcccta taccatctaa ttgcatttta tttacataaa tttgatagtt
    11761 tccacgacgt tcaaaaacag aaacacgtgc ttctaataag acttcatctc cttctttagg
    11821 ttcgaagttt aatttagaag cactaccttt gaacatcatg gcacttataa cgctctcttt
    11881 atctttcaca ttaaagtata aatgaccact tgaatgcttt ttgaaatttg aaagctcacc
    11941 tttaatcaat acagattgga gatgtggatc ttgatcaaat ttatatttaa tatatttcgt
    12001 taaagctgaa acac
//
LOCUS       DANBWH010000072        12002 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.075, whole genome shotgun sequence.
ACCESSION   DANBWH010000072 DANBWH010000000
VERSION     DANBWH010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12002
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.075"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            80..1417
                     /locus_tag="PPY92_001347"
     CDS             80..1417
                     /locus_tag="PPY92_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDJ2625359.1"
                     /translation="MKQPILNKLESLNQEEAISLHVPGHKNMTIGHLSQLSMTMDKTE
                     IPGLDDLHHPEEVILESMKQVEKHSDYDAYFLVNGTTSGILSVIQSFSQKKGDILMAR
                     NVHKSVLHALDISQQEGHFIETHQSPLTNHYNKVNLSRLNNDGHKLAVLTYPNYYGET
                     FNVEEVIKSLHQLNIPVLIDEAHGAHFGLQGFPDSTLNYQADYVVQSFHKTLPALTMG
                     SVLYIHKNAPYRETIIEYLSYFQTSSPSYLIMASLESAAEFYKTYDSTVFFDKRAQLI
                     ECLEKKGFEMLQVDDPLKLLIKYEGFTGHDIQNWFMNAHIYLELADDYQALAILPLWH
                     HDDTYLFDLLLRKIEDMILPKKSVSKVKQTQLLTTEGNYKPKRFEYVTWCDLKKAKGK
                     VLARHIVPYPPGIPIIFKGETITENMIELVNEYLETGMIVEGIKNNKILVEDE"
     gene            1419..2036
                     /locus_tag="PPY92_001348"
     CDS             1419..2036
                     /locus_tag="PPY92_001348"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDJ2625360.1"
                     /translation="MSAFITFEGPEGSGKTTVINEVYHRLVKDYDVIMTREPGGVPTG
                     EEIRKIVLEGNDMDIRTEAMLFAASRREHLVLKVIPALKEGKVVLCDRYIDSSLAYQG
                     YARGIGVEEVRALNEFAINGLYPDLTIYLNVSAEVGRERIIKNSRDQNRLDQEDLKFH
                     EKVIEGYQEIIHNESQRFKSVNADQPLENVVEDTYQTIIKYLEKI"
     gene            2064..2393
                     /locus_tag="PPY92_001349"
     CDS             2064..2393
                     /locus_tag="PPY92_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HDJ2625361.1"
                     /translation="MKMIIAIVQDQDSQELADQLVKNNFRATKLATTGGFLRAGNTTF
                     LCGVNDDRVDEILSVINQTCGNREQLVSPITPMGGSADSYIPYPVEVEVGGATVFVMP
                     VDAFHQF"
     gene            2607..3533
                     /locus_tag="PPY92_001350"
     CDS             2607..3533
                     /locus_tag="PPY92_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDJ2625362.1"
                     /translation="MDEQQQLTNAYHSNKLSHAYLFEGDDAQTMKQVAINFAKLILCQ
                     TDSQCETKVSTYNHPDFMYISTTENAIKKEQVEQLVRHMNQLPIESTNKVYIIEDFEK
                     LTVQGENSILKFLEEPPDNTIAILLSTKPEQILDTIHSRCQHVYFKPIDKEKFINRLV
                     EQNMSKPVAEMISTYTTQIDNAIALNEEFDLLALRKSVIRWCELLLTNKPMALIGIID
                     LLKQAKNKKLQSLTIAAVNGFFEDIIHTKVNVEDKQIYSDLKNDIDQYAQKLSFNQLI
                     LMFDQLTEAHKKLNQNVNPTLVFEQIVIKGVS"
     gene            3534..4337
                     /locus_tag="PPY92_001351"
     CDS             3534..4337
                     /locus_tag="PPY92_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDJ2625363.1"
                     /translation="MPNVIGVQFQKAGKLEYYTPNDIQVDIEDWVVVESKRGIEIGIV
                     KNPLMDIAEEDVVLPLKNIIRIADDKDIDKFNCNERDAENALILCKDIVREQGLDMRL
                     VNCEYTLDKSKVIFNFTADDRIDFRKLVKILAQHLKTRIELRQIGVRDEAKLLGGIGP
                     CGRSLCCSTFLGDFEPVSIKMAKDQNLSLNPTKISGACGRLMCCLKYENDYYEEVRAQ
                     LPDIGEAIETPDGNGKVVALNILDISMQVKLEGHEQPLEYKLEEIETMH"
     gene            4354..4701
                     /gene="yabA"
                     /locus_tag="PPY92_001352"
     CDS             4354..4701
                     /gene="yabA"
                     /locus_tag="PPY92_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HDJ2625364.1"
                     /translation="MDRNEIFEKIMRLEMNVNQLSKETSELKAHAVELVEENVALQLE
                     NDNLKKVLGNDEPTTIDTANSKPAKAVKKPLPSKDNLAILYGEGFHICKGELFGKHRH
                     GEDCLFCLEVLSD"
     gene            4975..5700
                     /locus_tag="PPY92_001353"
     CDS             4975..5700
                     /locus_tag="PPY92_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDJ2625365.1"
                     /translation="MLKENERFDQLIKEDFSIIQNDDVFSFSTDALLLGHFTKPRTKD
                     IVLDLCSGNGVIPLLLFAKHPRHIEGVEIQKTLVDMARRTFQFNDVDEYLTMHHMDLK
                     NVTKVFKPSQYTLVTCNPPYFKENQQHQHQKEAHKIARHEIMCTLEDCMIAARHLLKE
                     GGRLNMVHRADRLMDVLFEMRKVNIEPKKVVFIYSKVGKSAQTIVVEGRKGGNQGLEI
                     MPPFYIYNEDGNYSEEMKDVYYG"
     gene            5693..5941
                     /locus_tag="PPY92_001354"
     CDS             5693..5941
                     /locus_tag="PPY92_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDJ2625366.1"
                     /translation="MDSHFVYIVKCSDGSLYTGYAKDVNARVEKHNRGQGAKYTKVRR
                     PVHLVYQEMYETKSEALKREYEIKTYTRQKKLRLIKER"
     gene            5943..6782
                     /gene="rsmI"
                     /locus_tag="PPY92_001355"
     CDS             5943..6782
                     /gene="rsmI"
                     /locus_tag="PPY92_001355"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDJ2625367.1"
                     /translation="MAVLYLVGTPIGNLADITYRAVDVLKRVDMIACEDTRVTSKLCN
                     HYDIPTPLKSYHEHNKDKQTAFIIEQLELGLDVALVSDAGLPLISDPGYELVVAAREA
                     NIKVETVPGPNAGLTALMASGLPSYVYTFLGFLPRKEKEKSAVLEQRMHENSTLIIYE
                     SPHRVTDTLKTIAKIDATRQVSLGRELTKKFEQIVTDDVTQLQALIQQGDVPLKGEFV
                     ILIEGAKANNEISWFDDLSINEHVDHYIQTSQMKPKQAIKKVAEERQLKTNEVYNIYH
                     QIS"
     gene            7067..9040
                     /gene="metG"
                     /locus_tag="PPY92_001356"
     CDS             7067..9040
                     /gene="metG"
                     /locus_tag="PPY92_001356"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDJ2625368.1"
                     /translation="MAKETFYITTPIYYPSGNLHIGHAYSTVAGDVIARYKRMQGYDV
                     RYLTGTDEHGQKIQEKAQKAGKTEIEYLDEMIAGIKQLWAKLEISNDDFIRTTEERHK
                     HVVEQVFERLLKQGDIYLGEYEGWYSVPDETYYTESQLVDPQYENGKIIGGKSPDSGH
                     EVELVKEESYFFNISKYTDRLLEFYDQNPDFIQPPSRKNEMINNFIKPGLADLAVSRT
                     SFNWGVHVPSNPKHVVYVWIDALVNYISALGYLSDDESLFNKYWPADIHLMAKEIVRF
                     HSIIWPILLMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPNILIDRYGLDATRYY
                     LMRELPFGSDGVFTPEAFVERTNFDLANDLGNLVNRTISMVNKYFDGELPAYQGPLHE
                     LDEEMEAMALETVKSYTESMESLQFSVALSTVWKFISRTNKYIDETTPWVLAKDDSQK
                     DMLGNVMAHLVENIRYAAVLLRPFLTHAPKEIFEQLNINNPQFMEFSSLEQYGVLTES
                     IMVTGQPKPIFPRLDSEAEIAYIKESMQPPATEEEKEEIPSKPQIDIKDFDKVEIKAA
                     TIIDAEHVKKSDKLLKIQVDLDSEQRQIVSGIAKFYTPDDIIGKKVAVVTNLKPAKLM
                     GQKSEGMILSAEKDGVLTLVSLPSAIPNGAVIK"
     gene            9071..9844
                     /locus_tag="PPY92_001357"
     CDS             9071..9844
                     /locus_tag="PPY92_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDJ2625369.1"
                     /translation="MLIDTHVHLNDEQYDDDLSEVITRAREAGVDRMFVVGFNKSTIE
                     RAMKLIDEYDFLYGIIGWHPVDAIDFTEEHLEWIESLAQHPKVIGIGEMGLDYHWDKS
                     PADVQKEVFRKQIALAKRLKLPIIIHNREATQDCIDILLEEHAEEVGGIMHSFSGSPE
                     IADIVTNKLNFYISLGGPVTFKNAKQPKEVAKHVSMERLLVETDAPYLSPHPYRGKRN
                     EPARVTLVAEQIAELKGLSYEEVCEQTTKNAEKLFNLNS"
     gene            10011..10547
                     /gene="rnmV"
                     /locus_tag="PPY92_001358"
     CDS             10011..10547
                     /gene="rnmV"
                     /locus_tag="PPY92_001358"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDJ2625370.1"
                     /translation="MKINEFIVVEGRDDTERVKRAVECDTIETNGSAINEQTLEVIRN
                     AQQSRGVIVLTDPDFPGDKIRSTITEHVKGVKHAYIDREKAKNKKGKIGVEHADLIDI
                     KEALMHVSSPFDEAYESIDKSVLIELGLIVGKDARRRREILSRKLRIGHSNGKQLLKK
                     LNAFGYTEADVRQALEDE"
     gene            10558..11451
                     /gene="rsmA"
                     /locus_tag="PPY92_001359"
     CDS             10558..11451
                     /gene="rsmA"
                     /locus_tag="PPY92_001359"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDJ2625371.1"
                     /translation="MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIID
                     ASDIDAQTGVIEIGPGMGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVIN
                     EDILKANIKEAVENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEV
                     GERLNAEVGSKAYGSLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLV
                     TVDNEEAFFKLAKAAFAQRRKTINNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGET
                     LSIQDFAKLYEEKKKFPQLEN"
     gene            11551..11814
                     /locus_tag="PPY92_001360"
     CDS             11551..11814
                     /locus_tag="PPY92_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDJ2625372.1"
                     /translation="MPKSILDIKNSIDCHVGNRIVLKANGGRKKTIKRSGILKETYPS
                     VFIVELDQDKHNFERVSYTYTDVLTENVQVSFEEDNHHESIAH"
BASE COUNT         4375 a         1594 c         2372 g         3661 t
ORIGIN      
        1 tcaaagaagt gttactatat aattaatact tttgaaagta actaattcca aaacagtgta
       61 aataaaggaa gcgtatatca tgaagcaacc tattttaaat aaattagaaa gtttaaatca
      121 agaagaagcg atttctttgc atgttccggg tcataaaaat atgactatcg gtcatttatc
      181 tcaattatca atgacaatgg ataaaactga aatacctgga ttagatgatt tacatcatcc
      241 tgaagaagtc attttggaaa gtatgaagca ggtggagaaa cattcagatt atgatgctta
      301 tttcttagtg aatggcacca cttcaggaat attatctgtc atccagtctt tttcacagaa
      361 aaaaggcgat atcttaatgg caagaaatgt acataaatct gtgttacatg cgctcgatat
      421 tagccaacaa gaagggcatt ttattgaaac gcatcaaagt ccgttaacga atcattataa
      481 taaagttaat ttaagccgtt tgaataatga cggtcacaaa cttgctgtgt tgacttatcc
      541 taactattac ggtgaaacat ttaatgtaga agaggttatc aaatctttgc accaattaaa
      601 tattcctgta ctcattgacg aagcacacgg cgcgcacttt ggattgcaag gatttccaga
      661 ttctacatta aattatcaag ctgactatgt tgttcaatct tttcataaaa cgttaccagc
      721 tttaacgatg ggctcggtac tttatattca taaaaatgca ccttatagag aaactattat
      781 agaatatcta agctacttcc aaacatctag tccttcgtat ttgattatgg ctagtttaga
      841 gtcagctgcc gagttctata aaacatatga tagtaccgtg ttttttgata agagagcgca
      901 attaatcgaa tgtttggaga agaagggttt tgaaatgctt caagttgatg atccgttgaa
      961 gttgctgata aaatatgaag gttttacagg tcatgatatt caaaattggt ttatgaatgc
     1021 acatatctat ttagaattag cggacgacta tcaagcatta gcgatattgc cgttatggca
     1081 tcatgatgat acgtatttat ttgatttgct tttacgtaaa attgaagata tgattttacc
     1141 gaaaaaatca gtttctaaag ttaaacaaac acaactttta acaactgaag gtaactataa
     1201 accaaaacgc tttgaatatg ttacttggtg tgatttgaaa aaggcaaaag gtaaagttct
     1261 ggcgcgacat attgtcccgt atccgccagg gattcctatt attttcaaag gagaaacaat
     1321 aactgaaaat atgatagaat tggtaaatga atatctggaa actggaatga tagttgaagg
     1381 aattaaaaat aataaaattt tagttgagga tgaataaaat gtcagctttt ataacttttg
     1441 agggcccaga aggctctgga aaaacaactg taattaatga agtttaccat agattagtaa
     1501 aagattatga tgtcattatg actagagaac caggtggtgt tcctactggt gaagaaatac
     1561 gtaaaattgt attagaaggc aatgatatgg acattagaac tgaagcaatg ttatttgctg
     1621 catctagaag agaacatctt gtattaaagg tcataccagc tttaaaagaa ggtaaggttg
     1681 tgttgtgtga tcgctatatc gatagttcat tagcttatca aggttatgct agagggattg
     1741 gcgttgaaga agtaagagca ttaaacgaat ttgcaataaa tggattatat ccagacttga
     1801 cgatttattt aaatgttagt gctgaagtag gtcgcgaacg tattattaaa aattcaagag
     1861 atcaaaatag attagatcaa gaagatttaa agtttcacga aaaagtaatt gaaggttacc
     1921 aagaaatcat tcataatgaa tcacaacggt tcaaaagcgt taatgcagat caacctcttg
     1981 aaaatgttgt tgaagacacg tatcaaacta tcatcaaata tttagaaaag atatgatata
     2041 attgttagaa gaggtgttat aaaatgaaaa tgattatagc gatcgtacaa gatcaagata
     2101 gtcaggaact tgcagatcaa cttgttaaaa ataactttag agcaacaaaa ttggcaacaa
     2161 caggtgggtt tttaagagcg ggtaatacaa cattcttatg tggtgtcaat gatgaccgtg
     2221 tagatgaaat attgtctgtg attaatcaaa cgtgtggtaa tagagaacag ttggtttcac
     2281 ctattacacc tatgggaggc agtgcggatt cgtacattcc atatccagtt gaagttgaag
     2341 ttggcggtgc tactgtattt gttatgccag ttgatgcatt ccatcaattt taattctata
     2401 atacaatcat caattagaga tacttaaaat agtgtattaa taagttatta acaattttgg
     2461 gttgcttgac tgcgactagt tcagatgcca atagatttga tttttgtggt tctaaaaata
     2521 atcacaaatc atggtcgcta ttgttgcagt aattagttgc tctttggcaa cctttttata
     2581 taaaagcaaa agggagtttg taatgaatgg atgaacagca acaattgacg aatgcatatc
     2641 attcaaataa attatcgcat gcctatttat ttgaaggtga tgatgcacaa acgatgaaac
     2701 aagttgcgat taattttgca aagcttattt tatgtcaaac agatagtcaa tgtgaaacaa
     2761 aggttagtac atataatcat ccagacttta tgtatatatc aacaactgag aatgcaatta
     2821 agaaagaaca agttgaacaa cttgtgcgtc atatgaatca acttcctata gaaagcacaa
     2881 ataaagtgta catcattgaa gactttgaaa agttaactgt tcaaggggaa aacagtatct
     2941 tgaaatttct tgaagaacca ccggacaata cgattgctat tttattgtct acaaaacctg
     3001 agcaaatttt agacacaatc cattcaaggt gtcagcatgt gtatttcaag cctattgata
     3061 aagaaaagtt tataaataga ttagttgaac aaaacatgtc taagccagta gctgaaatga
     3121 ttagtactta tactacgcaa atagataatg caatagcttt aaatgaagaa tttgatttat
     3181 tagcattaag gaaatcagtt atacgttggt gtgaattgtt gcttactaac aagccaatgg
     3241 cacttatagg tattattgat ttattgaaac aggctaaaaa taaaaaactg caatctttaa
     3301 ctattgcagc tgtgaatggt ttcttcgaag atatcataca tacaaaggta aatgtagagg
     3361 ataaacaaat atatagtgat ttaaaaaatg atattgatca atatgcgcaa aagttgtcgt
     3421 ttaatcaatt aattttgatg tttgatcaac tgacggaagc acataagaaa ttgaatcaaa
     3481 atgtaaatcc aacgcttgta tttgaacaaa tcgtaattaa gggtgtgagt tagatgccaa
     3541 atgtaatagg tgttcagttt caaaaagcgg gaaaattaga atattataca cctaatgata
     3601 tacaagtaga tatagaagac tgggtagttg tcgaatctaa aagaggcata gagataggta
     3661 ttgttaaaaa tccattaatg gatattgctg aagaggatgt tgtgttacct cttaaaaata
     3721 ttattcgcat tgctgatgac aaagatattg ataaatttaa ttgtaatgaa cgagatgctg
     3781 aaaatgcatt aatactatgt aaagacattg taagagaaca aggtttggac atgcgtttag
     3841 tcaattgcga atatacatta gataaatcga aagttatttt taattttacg gcggatgatc
     3901 gtattgattt tagaaaatta gtaaaaatat tagcgcaaca tttaaaaaca cgtatcgagt
     3961 tgagacaaat tggtgtaagg gatgaagcca aattgcttgg cggtatcgga ccttgtggta
     4021 ggtcgttatg ttgttctaca tttttagggg attttgaacc agtatcgatt aagatggcta
     4081 aggatcaaaa tttatcatta aatccaacta aaatttctgg tgcatgtggt cgtttgatgt
     4141 gttgtttaaa atatgaaaat gactattatg aggaagtacg tgcacaatta cctgatattg
     4201 gtgaagcaat tgaaacgcct gatggtaacg ggaaagtagt tgctttaaat atattagaca
     4261 tttctatgca ggtgaagctt gagggacatg aacagccact tgaatataaa ttagaagaaa
     4321 tagaaactat gcattaagga ggcattatta catttggatc gcaatgaaat atttgaaaaa
     4381 ataatgcgtt tagaaatgaa tgtcaatcaa ctttcaaagg aaacttcaga attaaaggca
     4441 catgcagttg aattagtaga agaaaatgta gcgcttcaac ttgaaaatga taatttgaaa
     4501 aaggtgttgg gcaatgatga accaactact attgatactg cgaattcaaa accagcaaaa
     4561 gctgtgaaaa agccattacc aagtaaagat aatttggcta tattgtatgg agaaggattt
     4621 catatttgta aaggcgaatt atttggaaaa catcgacatg gtgaagattg tctgttctgt
     4681 ttagaagttt taagtgatta atcaagcaca ctcaaatagt gttataatta taaatgaata
     4741 tggtttggat aagtctgaga caatgcatgt ttcaggcttt aattgtgtat aaagttttgg
     4801 tgattgcata agagatggcg gtactaaatg ttattattaa gtgtgcacgc agtatcatta
     4861 gttataaaat gtagctgtta aaagtcaaaa atacatcgaa tgtagttagg catataatat
     4921 aaaaagagat ttcaattact caatagaaaa aggttgtctt cataggagtt aaaaatgtta
     4981 aaagagaatg aacgatttga tcaactaatc aaagaagatt ttagtattat tcaaaatgat
     5041 gatgtttttt cgttttcaac ggatgctttg ttgttagggc attttacaaa acctagaaca
     5101 aaagatattg tgttggactt atgttcaggc aatggggtga tacccttgtt attgtttgcg
     5161 aaacatccac gacatataga aggtgttgag attcaaaaaa cacttgtcga tatggcgcga
     5221 cgcacatttc aattcaatga tgttgatgaa tatttaacaa tgcatcacat ggatttgaaa
     5281 aacgttacta aagtatttaa accttcacaa tatactttag taacgtgtaa tccgccttat
     5341 tttaaagaga atcagcaaca ccaacatcaa aaagaagcac ataagatagc gagacatgag
     5401 attatgtgta cacttgaaga ttgcatgatt gcagcccgtc atttattaaa agaaggtggc
     5461 aggctaaata tggtacatcg tgcagataga ctaatggatg tcttgtttga aatgagaaaa
     5521 gtgaatattg aacctaagaa agtcgttttt atatatagta aggtagggaa atcagcacaa
     5581 acgatagtag tagaaggtcg aaaaggtgga aatcaaggtt tagaaatcat gcccccattt
     5641 tatatttata atgaagatgg taattatagc gaagaaatga aggacgtata ttatggatag
     5701 tcattttgta tatattgtaa aatgtagtga tggaagttta tatacaggat acgctaaaga
     5761 cgttaatgca cgtgttgaaa aacataaccg aggtcaagga gccaaatata cgaaagtaag
     5821 acgtccggtg catttagttt atcaagaaat gtatgagaca aagtctgaag cattgaagcg
     5881 tgaatatgaa attaaaactt ataccagaca aaagaaattg cgattaatta aggagcgata
     5941 gtatggctgt attatattta gtgggcacac caattggtaa tttagcagat attacttata
     6001 gagcagttga tgtattgaaa cgtgttgata tgattgcttg tgaagacact agagtaacta
     6061 gtaagctgtg taatcattat gatattccaa ctccattaaa gtcatatcac gaacataaca
     6121 aggataagca gactgctttt atcattgaac agttagaatt aggtcttgac gttgcgctcg
     6181 tatctgatgc tggattgccc ttaattagtg atcctggata cgaattagta gtggcagcca
     6241 gagaagctaa tattaaagta gagactgtgc ctggacctaa tgctgggctg acggctttga
     6301 tggctagtgg attaccttca tatgtatata catttttagg atttttgcca cgaaaagaga
     6361 aagaaaaaag tgctgtatta gagcaacgta tgcatgaaaa tagcacatta attatatacg
     6421 aatcaccgca tcgtgtgaca gatacattaa aaacaattgc aaagatagat gcaacacgac
     6481 aagtatcact agggcgtgaa ttaactaaga agttcgaaca aattgtaact gatgatgtaa
     6541 cacaattaca agcattgatt cagcaaggcg atgtaccatt gaaaggcgaa ttcgttatct
     6601 taattgaagg tgctaaagcg aacaatgaga tatcgtggtt tgatgattta tctatcaatg
     6661 agcatgttga tcattatatt caaacttcac agatgaaacc aaaacaagct attaaaaaag
     6721 ttgctgaaga acgacaactt aaaacgaatg aagtatataa tatttatcat caaataagtt
     6781 aatcacttta tcgattatat gaaattttaa acgattttat aaacgcaagc tgtaatttta
     6841 aatggtaagt tatcattttg cattgatact gataaaatga tgttgactat gataaaaaaa
     6901 tgatgacatc gacgtttttt aatgtaaaat aaatacattg aaagtaataa ataccttaac
     6961 attgaataag atgaaaatga gatgacgaga taaatgttcg cgtccgttga aatgcataga
     7021 aatcttagat attatttgaa gtgagacatt acgaggagga acagttatgg ctaaagaaac
     7081 attttatata acaaccccaa tatactatcc tagtgggaat ttacatatag gacatgcata
     7141 ttctacagtg gctggagatg ttattgcaag atataagaga atgcaaggat atgatgttcg
     7201 ttatttgact ggaacggatg aacacggtca aaaaattcaa gaaaaagctc aaaaagctgg
     7261 taagacagaa attgaatatt tggatgagat gattgctgga attaaacaat tgtgggctaa
     7321 gcttgaaatt tcaaatgatg attttatcag aacaactgaa gaacgtcata aacatgtcgt
     7381 tgagcaagtg tttgaacgtt tattaaagca aggtgatatc tatttaggtg aatatgaagg
     7441 ttggtattct gttccggatg aaacatacta tacagagtca caattagtag acccacaata
     7501 cgaaaacggt aaaattattg gtggcaaaag tccagattct ggacacgaag ttgaactagt
     7561 taaagaagaa agttatttct ttaatattag taaatataca gaccgtttat tagagttcta
     7621 tgaccaaaat ccagatttta tacaaccacc atcaagaaaa aatgaaatga ttaacaactt
     7681 cattaaacca ggacttgctg atttagctgt ttctcgtaca tcatttaact ggggtgtcca
     7741 tgttccgtct aatccaaaac atgttgttta tgtttggatt gatgcgttag ttaactatat
     7801 ttcagcatta ggctatttat cagatgatga gtcactattt aacaaatact ggccagcaga
     7861 tattcattta atggctaagg aaattgtgcg attccactca attatttggc ctattttatt
     7921 gatggcatta gacttaccgt tacctaaaaa agtctttgca catggttgga ttttgatgaa
     7981 agatggaaaa atgagtaaat ctaaaggtaa tgttgtagac cctaatattt taattgatcg
     8041 ctatggttta gatgctacac gttattatct aatgcgtgaa ttaccatttg gttcagatgg
     8101 cgtatttaca cctgaagcat ttgttgagcg tacaaatttc gatctagcaa atgacttagg
     8161 taacttagta aaccgtacga tttctatggt taataagtac tttgatggcg aattaccagc
     8221 gtatcaaggt ccacttcatg aattagatga agaaatggaa gctatggctt tagaaacagt
     8281 gaaaagctac actgaaagca tggaaagttt gcaattttct gtggcattat ctacggtatg
     8341 gaagtttata agtagaacga ataagtatat tgacgaaaca acgccttggg tattagctaa
     8401 ggacgatagc caaaaagata tgttaggcaa tgtaatggct cacttagttg aaaatattcg
     8461 ttatgcagct gtattattac gtccattctt aacacatgcg ccgaaagaga tttttgaaca
     8521 attgaacata aacaatcctc aatttatgga atttagtagt ttagagcaat atggtgtgct
     8581 tactgagtca attatggtta ctgggcaacc taaacctatt ttcccaagat tggatagcga
     8641 agcggaaatt gcatatatca aagaatcaat gcaaccgcct gctactgaag aggaaaaaga
     8701 agagattcct agcaaacctc aaattgatat taaagacttt gataaagttg aaattaaggc
     8761 agcaacgatt attgatgctg aacatgttaa gaagtcagat aagcttttaa aaattcaagt
     8821 agacttagat tctgaacaaa gacaaattgt atcaggaatt gccaaattct atacaccaga
     8881 tgatattatt ggtaaaaaag tagcagttgt tactaacctg aaaccagcta aattaatggg
     8941 acaaaaatct gaaggtatga tattatctgc tgaaaaagat ggtgtattaa ccttagtaag
     9001 tttaccaagt gcaattccaa atggtgcagt gattaaataa ctgtattttt aaaaattagg
     9061 agagataatt atgttaatcg atacacatgt ccatttaaat gatgagcaat acgatgatga
     9121 tttgagtgaa gtgattacac gtgctagaga agcaggtgtt gatcgtatgt ttgtagttgg
     9181 ttttaacaaa tcgacaattg aacgcgcgat gaaattaatc gatgagtatg attttttata
     9241 tggcattatc ggttggcatc cagttgacgc aattgatttt acagaagaac acctggaatg
     9301 gattgaatct ttagctcagc atccaaaagt gattggtatt ggtgaaatgg gattagatta
     9361 tcactgggat aaatctcctg cagatgttca aaaggaagtt tttagaaagc aaattgcttt
     9421 agctaagcgt ttgaagttac caattatcat tcataaccgt gaagcaactc aagactgtat
     9481 cgatatctta ttggaggagc atgctgaaga ggtaggcggg attatgcata gctttagtgg
     9541 ttctccagaa attgcagata ttgtaactaa taagctgaat ttttatattt cattaggtgg
     9601 acctgtgaca tttaaaaatg ctaaacagcc taaagaagtt gctaagcatg tgtcaatgga
     9661 gcgtttgcta gttgaaaccg atgcaccgta tctctcgcca catccgtata gagggaagcg
     9721 aaatgaaccg gcgagagtaa ctttagtagc tgaacaaatt gctgaattaa aaggcttatc
     9781 ttatgaagaa gtgtgcgaac aaacaactaa aaatgcagag aaattgttta atttaaattc
     9841 ataaagttaa aagtgagaaa gatcaccgcc ataaatgtaa acgatgctat attcgtttaa
     9901 tatgctatgg ttctttctca cttttttaaa ttaaaatatc gtacatgtgc aatacgtacg
     9961 atagagatgg ttagagcttt gaaattaaga attgtaggaa ggcgttttaa atgaaaatca
    10021 atgagtttat agttgtagaa ggacgagatg atactgagcg tgttaaacga gctgttgaat
    10081 gtgatacgat tgaaacgaat ggtagtgcca tcaacgaaca aactttagaa gtaattagaa
    10141 atgctcaaca aagtcgaggc gttattgtat taacagatcc agatttccca ggagataaaa
    10201 ttagaagtac aattactgaa catgtcaaag gtgttaaaca tgcgtatatt gatagagaaa
    10261 aagctaaaaa taaaaaaggg aaaattggtg ttgaacatgc cgacttaatt gatattaaag
    10321 aagcgttaat gcatgttagt tcaccctttg atgaagctta tgaatcaatt gataaatctg
    10381 tgctaataga gttggggtta atcgttggga aagatgcaag gcgccgtaga gaaattttaa
    10441 gtagaaaatt gcgaatcggc cattccaatg gtaagcagtt attgaaaaag ttaaatgcat
    10501 ttggttatac cgaagcggat gtaaggcaag ctttagaaga tgaatgagga agtgaaaatg
    10561 ttggataata aagatattgc aacaccatca agaacgcgag cgttgttaga taaatatggc
    10621 tttaatttta aaaaaagttt aggacagaac tttttgatag atgtgaatat cattaataat
    10681 atcattgatg caagtgatat tgatgcacaa actggggtga ttgaaattgg tccaggtatg
    10741 gggtcgttga cagaacaatt ggccagacat gctaaaagag tattggcatt tgaaattgat
    10801 caacgtttaa tacctgtatt aaatgatact ctatcacctt atgataatgt gacggtgatt
    10861 aatgaagata ttttaaaagc gaatattaaa gaagctgttg aaaatcattt acaagattgc
    10921 gaaaaaataa tggttgttgc aaacctgccg tactatatta cgacgccaat tttattaaat
    10981 ttaatgcaac aagatatacc aattgatggc tacgtggtga tgatgcaaaa agaagtgggc
    11041 gaacgcttaa atgctgaagt aggttcaaaa gcatatggtt cattatcaat tgtcgtacaa
    11101 tactatacag agactagtaa agtattaacg gtacctaaat ctgtatttat gccaccacct
    11161 aatgttgatt caatagttgt aaaactgatg cagagaactg aaccgttagt aacagtagat
    11221 aacgaggaag cattctttaa gttagcaaaa gcagcatttg cacaaagaag aaagacaatt
    11281 aacaataact atcaaaatta ttttaaagat ggtaaacaac acaaagaagt gattttacaa
    11341 tggttggaac aagcaggtat tgatccaaga cgtcgcggtg aaacgctatc tattcaagat
    11401 tttgctaaat tgtatgaaga aaagaaaaaa ttccctcaat tagaaaatta aatgattgac
    11461 aaagcaaagc actattgtta aaatttaaat tttgtttgac gaaaacgctg caaatatggt
    11521 attatgtaac ttgtagcgag gtggagcaat atgccaaaat caattttgga catcaaaaat
    11581 tctattgatt gtcatgtagg aaatcgtatt gtactgaaag ccaatggagg ccgtaagaaa
    11641 acaataaaac gttctggaat tttaaaagaa acatatccgt cagttttcat tgttgagtta
    11701 gatcaagaca aacacaactt tgagagagta tcttatacat acactgatgt gttaactgaa
    11761 aatgttcaag tttcatttga agaggataat catcacgaat caattgcaca ctaaataaga
    11821 catatagaga tgttaggcgt ttcttagtat aagaagtaaa tattatgata attatttgag
    11881 tgttgggcat tatgttcaat actcttttta tttacaaaat gtttaacact gatgtttcgc
    11941 ttatagattt ttcagtaaat ggataattgt atttataaac acaaatacaa gtaaatacta
    12001 ag
//
LOCUS       DANBWH010000073        11894 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.076, whole genome shotgun sequence.
ACCESSION   DANBWH010000073 DANBWH010000000
VERSION     DANBWH010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11894
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.076"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..533)
                     /gene="radC"
                     /locus_tag="PPY92_001361"
     CDS             complement(<1..533)
                     /gene="radC"
                     /locus_tag="PPY92_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDJ2625373.1"
                     /translation="MKIKEMVTSEMPRERLLSHGAKSLSNTELLAILINTGRKGFSSI
                     DISNELLKSASNLNELKKSSINDLIKVKGIGLQKAITLKAAFELGERMGRRAENNRIK
                     ITQPSDVADYMIPTMKDLTQEHFVILLLNSKNVVIKETCVFKGTLNSSIVHPREIFSI
                     AVRENANAIIAVHNHPSG"
     gene            complement(530..1237)
                     /locus_tag="PPY92_001362"
     CDS             complement(530..1237)
                     /locus_tag="PPY92_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDJ2625374.1"
                     /translation="MVVLLSYSCSCIFSFLYQFISIEETSFDYLHRRSKCDYCNSSLK
                     WYELMPIISFLLLKGRCRNCRKRISLTHFLGETFALIPIVFIKYDFTYVNATLFITTY
                     VFLLIFTMTDITSLMLDCRLIIIYCIVSLSLSMIYPVAFIIISMTTHIFYFLFRAYIG
                     YGDVLLISALSLFFPLQFTIYVILFTFVIAGLVALITMIFKPIKLLPLVPFIFISFFI
                     NSLFYNDIHQFLGGVYF"
     gene            complement(1507..2778)
                     /locus_tag="PPY92_001363"
     CDS             complement(1507..2778)
                     /locus_tag="PPY92_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDJ2625375.1"
                     /translation="MNYLESLYWIHERTKFGIKPGVKRMEWMLAQFNNPQNNIKGIHV
                     GGTNGKGSTVAYLRTALVENGYEVGTFTSPFIETFNERISLNGVPISNDAIVELVSRI
                     KPVSEMMERETDLGVATEFEIITAMMFLYFGEIHPVDFVIVEAGLGIKNDSTNVFTPV
                     LSILTSIGLDHTDILGGTYLDIARDKGAIIKPNVPVIYAVKNEDALKYVRERAIEQHA
                     KPIELDREIVVVSQNDEFTYRYKDYELETIILSMLGEHQKQNAALAITALIELNEQGL
                     IELDFNKMIDGIESVRWTGRIEQVHDKPLIILDGAHNSESIDALIDTIKQYHDKEKVD
                     ILFSAINGKPINEMVKHLSLIAHTFYATEFDFPKALRKEEIVGSIENDEIQLVDDYVE
                     FIKNYQGDTLVITGSLYFISEVKSKLDFEAL"
     gene            complement(2791..5421)
                     /locus_tag="PPY92_001364"
     CDS             complement(2791..5421)
                     /locus_tag="PPY92_001364"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDJ2625376.1"
                     /translation="MEMKPKYDPREVEAGRYEEWVKNGYFKPSEDKSKETYTIVIPPP
                     NVTGKLHLGHAWDTTLQDIITRMKRMQGYDTLYLPGMDHAGIATQAKVEAKLNEQGIT
                     RYDLGREKFLEQAWDWKEEYASFIRAQWAKLGLGLDYSRERFTLDEGLSKAVKKVFVD
                     LYNKGIIYRGERIINWDPKARTALSDIEVIHEDVQGAFYHFKYPYADGEGFIEIATTR
                     PETMLGDTAIVVNPNDERYKDVIGKTVILPIVGRELPILADEYVDIDFGSGAMKVTPA
                     HDPNDFEIGQRHQLENIIVMDENGKMNDKAGKYEGMDRFDCRKQLVKDLKEQDLVIKI
                     EDHVHSVGHSERSGAVVEPYLSTQWFVRMEDLAKRSLDNQKTDDRIDFYPQRFEHTFN
                     QWMENIRDWTISRQLWWGHQIPAWYHKETGEIYVGEEAPTDIENWQQDEDVLDTWFSS
                     ALWPFSTLGWPDLESEDFKRYYPTNALVTGYDIIFFWVARMIFQGLEFTDRRPFNDVL
                     LHGLVRAEDGRKMSKSLGNGVDPMDVIDEYGADSLRYFLATGSSPGHDLRYSTEKVES
                     VWNFINKIWNGARFSLMNIGEDFKVEDIDLSGNLSLADKWILTRLNETIATVTDLSDK
                     YEFGEVGRALYNFIWDDFCDWYIEMSKIPMNSNDEEQKQVTRSVLSYTLDNIMRMLHP
                     FMPFVTEKIWQSLPHEGDTIVKASWPEVRESLIFEESKQTMQQLVEIIKSVRQSRVEV
                     NTPLSKEIPILIQAKDKEIETTLSQNKDYLIKFCNPSTLNISTDVEIPEKAMTSVVIA
                     GKVVLPLEGLIDMDKEISRLEKELAKLQSELDRVDKKLSNENFVSKAPEKVINEEKRK
                     KQDYQEKYDGVKARIEQLKA"
     misc_binding    complement(5465..5766)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5824..6384
                     /locus_tag="PPY92_001365"
     CDS             5824..6384
                     /locus_tag="PPY92_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDJ2625377.1"
                     /translation="MNECAFGTKDPVYLNYHDHVWGQPLYDSKALFKLLALESQHAGL
                     SWLTILKKKEAYEEAFYDFEPEKVAQMTAQDIDRLMTFPNIVHHRKKLEAIVNQAQGY
                     LKIEQAYGSFSKFLWSYVNGKPKDLQYEHASDRITVDDTATQLSKDLKQYGFKFLGPV
                     TVFSFLEAAGLYDAHLKDCPSKPKHN"
     gene            complement(6607..7680)
                     /locus_tag="PPY92_001366"
     CDS             complement(6607..7680)
                     /locus_tag="PPY92_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDJ2625378.1"
                     /translation="MTMIYRNNFIVFVLSFFISIILYSSHVLLPFMFGPIIASIICVK
                     VFKLDIKWPFLLSELGIVLLGVQIGSTFTKNVVMDIKNNWLSIIVVSISILLIAIVMA
                     FIFKKIARINTETAILSVIPGALTQMLVMAEQDKRANLLVVSLTQTSRIIFVVVLVPF
                     ISYFFHDGNMHANGKLTKVLPLSQVLNIGQIVILAIAIFIVYLIMSKIKFPTFQLLAP
                     LIVLIVWNFSTGLTFTLDHWLLNMAQLIYMIRIGVQIAHLLSDLKGRLAIAITIQNIM
                     LIIGALIMVYIIHFFDNNPINELFLGAAPGGVNQIVLVALATGADVAMITSYHIFRIF
                     FILFIIAPGINYFLKYRSNKRED"
     gene            complement(7714..7815)
                     /locus_tag="PPY92_001367"
     CDS             complement(7714..7815)
                     /locus_tag="PPY92_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625379.1"
                     /translation="MGGDLIVKVHICFVVKTASGYCYLNKREAQAAI"
     gene            complement(7876..9162)
                     /gene="hemL"
                     /locus_tag="PPY92_001368"
     CDS             complement(7876..9162)
                     /gene="hemL"
                     /locus_tag="PPY92_001368"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDJ2625380.1"
                     /translation="MRYTKSEEAMKVAETLMPGGVNSPVRAFKSVDTPAIFMDHGKGS
                     KIYDIDGNEYIDYVLSWGPLILGHRDPQVISHLHEAIDKGTSFGASTLLENKLAQLVI
                     DRVPSIEKVRMVSSGTEATLDTLRLARGYTGRNKIVKFEGCYHGHSDSLLIKAGSGVA
                     TLGLPDSPGVPEGIAKNTITVPYNDLDALKIAFEKFGDDIAGVIVEPVAGNMGVVPPI
                     EGFLQGLRDITTEYGALLIFDEVMTGFRVGYHCAQGYFGVTPDLTCLGKVIGGGLPVG
                     AFGGKKEIMDHIAPLGNIYQAGTLSGNPLAMTSGYETLSQLTPETYEYFNMLGDILED
                     GLKRVFAKHNVPITVNRAGSMIGYFLNEGPVTNFEQANKSDLKLFAEMYREMAKEGVF
                     LPPSQFEGTFLSTAHTKEDIEKTIQAFDTALSRIVK"
     gene            complement(9210..10184)
                     /gene="hemB"
                     /locus_tag="PPY92_001369"
     CDS             complement(9210..10184)
                     /gene="hemB"
                     /locus_tag="PPY92_001369"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001633925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDJ2625381.1"
                     /translation="MKFDRHRRLRSSATMRDMVRENHVRKEDLIYPIFVVEKDDVKKE
                     IKSLPGVYQISLNLLESELKEAYDLGIRAIMFFGVPNSKDDIGTGAYIHDGVIQQATR
                     IAKKMYDDLLIVADTCLCEYTDHGHCGVIDDHTHDVDNDKSLPLLVKTAISQVEAGAD
                     IIAPSNMMDGFVAEIRRGLDEAGYYNIPIMSYGVKYASSFFGPFRDAADSAPSFGDRK
                     TYQMDPANRLEALRELESDLKEGCDMMIVKPALSYLDIVRDVKNHTNVPVVAYNVSGE
                     YSMTKAAAQNGWIDEERVVMEQMVSMKRAGADMIITYFAKDICRYLDK"
     gene            complement(10187..10855)
                     /locus_tag="PPY92_001370"
     CDS             complement(10187..10855)
                     /locus_tag="PPY92_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDJ2625382.1"
                     /translation="MKPVVVMTQTNDMQSDLVSIIHKPFIDIKPLNFDIHLLNQRYDW
                     LIFSSKNAVKFFYKYLKGINVDNIAVIGSKTAQYCESLGIRVDFMPNDFSQEGFLKSF
                     NQTNQKILLPSSELARPLLLAALSKDNEVVKIDLYTSVPNKQNIQDVKEMIEHQQIDA
                     LTFSSSSAVRYYFNEGFVPKFKSYFAIGEQTARTIKTYQQPVTIAEIQTLESLIEKIL
                     ESRG"
     gene            complement(10877..11803)
                     /gene="hemC"
                     /locus_tag="PPY92_001371"
     CDS             complement(10877..11803)
                     /gene="hemC"
                     /locus_tag="PPY92_001371"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDJ2625383.1"
                     /translation="MRKLVVGSRRSKLALTQSQQFINKLKAVEPNLEIEIKEIVTKGD
                     RIVDKQLSKVGGKGLFVKEIQHELFEKNIDMAIHSLKDVPSVIPEGLTLGCIPDRELP
                     FDAYISKTHTPLSQLPEGSIIGTSSLRRGAQILSKYPNLEIKWIRGNIDTRLEKLQTE
                     DYDAIILAAAGLRRMGWSDDIVTSYLDRDTLLPAIGQGALGIECRSDDEELLTLLSKV
                     HNDEVAKCVTAERTFLAEMDGSCQVPIAGYATISDQKEIEFTGLIMTPDGKERFEYTM
                     NGTDPVELGKTVSNKLKEQGAYEIIKRLNEQH"
BASE COUNT         3871 a         2273 c         1643 g         4107 t
ORIGIN      
        1 ccggatggat gattatgaac tgcgatgatt gcattggcat tttctctcac cgcaatacta
       61 aaaatttcac gtggatgtac aatcgaacta tttaatgtac ctttaaaaac acaggtttct
      121 ttaatcacta cattttttga atttaacaat aaaatgacaa aatgttcttg tgttaaatct
      181 ttcattgttg gaatcatata atcagcaaca tcacttggtt gcgttatttt tatacgatta
      241 ttttcagctc ttctccccat cctttcccct aactcaaatg ctgcttttaa agtaattgct
      301 ttttgtaatc caatcccttt aacttttatc aaatcgttaa ttgaagattt tttcaattca
      361 ttcagattcg aagcagattt aagcagttca ttactaatgt ctatgctcga gaatcctttt
      421 cttccggtgt taattaatat agctaataat tctgtattcg aaagactttt tgcaccatgg
      481 cttaacaaac gttctcttgg catttctgaa gttaccattt ctttaatttt caaaaatata
      541 cgcctcctaa aaattgatgg atatcattat aaaaaagtga attgataaaa aaggaaataa
      601 atataaatgg aacaaggggt aatagtttaa tcggcttaaa tatcatggta attaaagcaa
      661 ctaaaccagc aatgacaaat gtaaataaaa tgacataaat agtgaattgg agagggaaaa
      721 acaaagaaag tgcagatatt agtaaaacgt caccataacc aatatatgcc cgaaataaaa
      781 agtagaatat atgcgtggtc atactaataa tgataaaagc tactggataa atcatactta
      841 acgagagaga aacgatacaa taaattataa ttaagcgaca atctaacatt aaagaagtga
      901 tatcggtcat agtaaaaata agcagaaaaa catatgtagt tataaatagc gtagcattta
      961 cgtatgtgaa atcatactta ataaagacga taggtattaa agcaaaggtt tcccctaaga
     1021 aatgtgttag ggaaatacgc tttcgacagt ttcgacatcg cccttttaat aataaaaaac
     1081 taataatcgg cattaattca taccatttga gtgacgaatt acaataatca catttcgatc
     1141 ttctatgtaa ataatcaaat gacgtttctt ctatagatat aaattgatat agaaaactaa
     1201 aaatacaact gcagctataa gataacaata ctaccaaatg acaacctcct tatgtaaatt
     1261 atagttagtt attaccaaaa tgtaaatata cactattttt caagaattga accgcttttt
     1321 catttaaatt tttcaatatt gctaagcata attgatggat actttaacaa cccattactg
     1381 ctcggcaaaa ttaataatgg caagaaattg aaccttataa acacatacga tttagagcat
     1441 aaaaaataac catgaagctc tacctattga ttaaatagat tcttcatggc tattttagtt
     1501 ttagttttat aatgcttcaa agtctaattt tgatttaact tcacttatga aatacagact
     1561 accggtaatt actaatgtat caccttgata attttttata aattcaacgt agtcatctac
     1621 taattgtatt tcatcatttt caatactacc tacaatttct tctttgcgta acgctttcgg
     1681 aaaatcaaat tcagttgcat aaaacgtatg cgcaattaaa cttaaatgtt tgaccatctc
     1741 gttaatcggt tttccgttta ttgctgagaa caaaatatct actttttctt tatcatggta
     1801 ctgtttaatt gtatcaatta gagcatctat actctctgaa ttatgtgcgc catccaaaat
     1861 gattaaaggt ttgtcatgca cctgctcaat acgtccagtc caacgaactg attcaatacc
     1921 gtctatcatc ttattgaaat ctaattcaat taatccttgt tcatttaatt caataagagc
     1981 tgttatggct aatgcagcat tttgtttctg atgttcacct aacatgctta aaatgattgt
     2041 ttctaattca taatctttat aacggtaagt aaattcatca ttttgcgata caacaacaat
     2101 ttctctatct aattcaattg gctttgcatg ttgttcaatt gcgcgttcac gaacatattt
     2161 taatgcatct tcatttttaa cagcatatat cactggaacg ttaggcttta taatcgcgcc
     2221 tttatcccta gcaatatcta gataagtacc acctaaaata tctgtatggt ctagaccgat
     2281 actagttaag attgataaaa ccggtgtaaa gacatttgtc gaatcgttct ttatacccaa
     2341 tccagcctca acaatgacaa aatcaacagg atgtatttca ccaaaatata aaaacatcat
     2401 cgctgtgatt atttcgaatt cagttgcaac acctaaatct gtttcacgtt ccatcatttc
     2461 acttactggt ttaatacgtg atactaattc tacaatagcg tcatttgata ttggcacacc
     2521 atttagacta attcgttcat taaatgtttc aataaacggc gacgtaaatg tacctacttc
     2581 ataaccattt tcaactaaag ctgttctaag gtaagcaact gtagagcctt taccatttgt
     2641 gccacctaca tgaataccct taatgttatt ttgaggatta ttaaattgtg ctagcatcca
     2701 ttccatacgt ttaacacctg gtttgatgcc aaatttagtt ctttcgtgta tccaatacaa
     2761 gctctctagg taattcattg ttactaactc ctatgctttt aattgttcaa ttcttgcctt
     2821 cacaccatca tatttttctt gataatcttg ttttttacgt ttttcttcat ttataacctt
     2881 ttcaggtgct ttacttacaa agttttcatt agagagcttt ttatctactc tatctaattc
     2941 gctttgaagt ttagctaatt ctttttccaa acggctgatt tccttatcca tatcaattag
     3001 cccttctaat ggtaatacca ctttacctgc aattacaact gatgtcattg ctttctcagg
     3061 aatttccacg tcagtgctaa tatttaaggt actaggatta cagaatttga ttaaataatc
     3121 tttgttttgt gataaagttg tttcaatttc tttatcttta gcttgaatta aaataggtat
     3181 ttctttagac aatggcgtat ttacttctac acgtgattgt cttacagatt taatgatttc
     3241 aacaagttgt tgcattgttt gtttactttc ttcaaaaatc aatgattcac gcacttctgg
     3301 ccatgaagct ttaacaattg tgtcaccttc atgtggtaaa ctttgccata ttttctctgt
     3361 tacaaatggc atgaatggat gtagcattct cataatattg tctaaagtat aactcaatac
     3421 tgaacgtgta acttgttttt gttcttcatc attactattc attggaattt tactcatttc
     3481 aatgtaccaa tcacagaaat catcccaaat gaaattatat aatgcacgtc caacttcgcc
     3541 gaattcatat ttgtcactta aatcagtaac tgttgcaatc gtttcattta aacgtgttag
     3601 aatccattta tctgctaatg ataagttacc acttaaatcg atatcttcaa ctttaaagtc
     3661 ttcaccgata ttcattaaac tgaaacgtgc cccattccag attttattga taaagttcca
     3721 cactgactca actttttcag ttgagtatct taaatcatgt cctggagatg aacctgttgc
     3781 taagaagtaa cgcaagctat cagcaccgta ttcgtcaata acatccattg gatccacacc
     3841 attacctaat gatttactca tcttacgccc gtcttcagca cgaactaaac cgtgtaataa
     3901 tacatcatta aatggacgac gatctgtaaa ttctaagcct tggaatatca tgcgtgcgac
     3961 ccagaaaaag ataatatcgt aacctgtaac taaggcattt gttgggtagt atcgtttaaa
     4021 gtcttcactt tctaaatcag gccaacctaa cgtagagaaa ggccataaag cacttgagaa
     4081 ccacgtatct aatacatctt catcttgttg ccaattttca atatcagttg gcgcttcttc
     4141 tccaacatat atttcgcctg tttctttatg ataccaagcc ggaatttgat gaccccacca
     4201 taattgtctt gaaatcgtcc aatctctaat attttccatc cattggttaa atgtatgttc
     4261 gaaacgttgc ggataaaaat caatacgatc atctgttttt tggttatcta atgaacgttt
     4321 cgctaagtct tccatgcgca caaaccattg tgttgataaa tatggttcaa caacagcgcc
     4381 agatcgttct gaatgaccta cagaatgaac atgatcttca atcttgataa ctaaatcttg
     4441 ttcttttaaa tctttaacta gctgtttacg acaatcaaaa cggtccatac cttcatattt
     4501 acccgctttg tcgttcattt taccattttc atccataacg ataatatttt ctaattgatg
     4561 tctttgacca atttcaaaat cattagggtc atgtgctggt gtcactttca tagcaccaga
     4621 accgaagtct atatcaacat actcatctgc taaaataggc agttcgcgtc ctacgattgg
     4681 taatataaca gttttaccga ttacatcttt gtatcgttcg tcattagggt taacaacaat
     4741 cgctgtatca cctaacatcg tttctggtct tgttgttgca atttcaataa aaccttcacc
     4801 atcagcgtaa ggatatttaa aatgataaaa cgcaccttga acatcttcat gtattacttc
     4861 aatatcagat aaagctgtac gtgctttagg atcccaattt ataatacgtt cgccacgata
     4921 aataattcct ttattgtata aatcaacaaa aactttttta actgctttac ttaaaccttc
     4981 atctaaagta aaacgttctc tactataatc taaacctaga cctaatttag cccattgcgc
     5041 acgaataaat gacgcatact cttctttcca atcccatgcc tgttctaaaa acttttcacg
     5101 accaagatca tatctagtta ttccttgttc atttaattta gcttctacct ttgcctgtgt
     5161 cgcaatacca gcatgatcca tacctggtaa gtataacgta tcgtatcctt gcatacgttt
     5221 catacgtgta atgatatctt gtaaagtcgt atcccatgca tgtcctaaat gtaatttacc
     5281 agttacattt ggtggcggga taacaattgt atatgtttct tttgatttat cttctgacgg
     5341 tttaaaataa ccattcttta cccattcttc ataacgtccc gcttcaactt cacgaggatc
     5401 atattttggt ttcatttcca tttgctatac ctcctaaaaa aataaaaata tccatcctat
     5461 atacaaatag gacggatatt ccgtggtacc acctatattc aagaaggatg tttaatatca
     5521 aattcactct tttaacataa ttggaataat cataccaata ctatcatcgt gaaatttgaa
     5581 atgcttcatc tcttcaagca ctctagatta tgattaacgc tcaaacacgt cttagcctac
     5641 tattaatcac gttcagctaa gatactctgt gggctacctt cagtaaaaat catttacata
     5701 ctcacaccaa atcatatgct ctctttaaaa taatttgaac ttactcttcc caaatcctat
     5761 attaaactct taacttatag tataatgatt gacaaaataa gtcaatgtat aggtgggaat
     5821 aaaatgaatg aatgcgcatt tggtactaaa gatccagtct acttaaacta tcatgatcat
     5881 gtatggggac aaccgctcta tgatagcaag gcattgttta aacttttagc attagaatca
     5941 caacatgctg ggctatcttg gttaactatt ttaaaaaaga aagaagccta tgaagaagca
     6001 ttttatgatt tcgaaccaga aaaggtagca caaatgaccg ctcaagatat cgaccgctta
     6061 atgacttttc caaatatcgt tcatcatcgt aaaaaattag aagcaattgt taatcaagct
     6121 caagggtatt taaaaattga acaagcatat ggtagtttta gtaaattttt atggtcttat
     6181 gtaaatggta agcctaaaga tttgcagtat gaacatgctt ctgatcgtat cacagttgat
     6241 gatactgcaa cacaactatc taaagattta aaacaatacg ggtttaaatt tttaggtcca
     6301 gtaacagtat tttcgttttt agaagcagct ggtttatatg atgcacattt aaaagattgt
     6361 ccatcaaagc ctaaacacaa ttaatttgct atagcatcat aaagtaaacg agacatcatc
     6421 tatttttaac taatagactg tctcgttttt tgatgtgaaa atttattcaa atcataagtt
     6481 aaagttccaa gttaatgcac tgctaatatt agatatctta atctaaaaat agatgtacaa
     6541 cctttgctta aaccttaata atgaattcta tcctaagcat aattcaactt aaatggatga
     6601 tagtttctaa tcttcacgct tattcgaacg atattttaaa aagtaattaa ttcctggtgc
     6661 aataataaat aggatgaaaa agattcggaa aatatgataa ctcgtaatca tagcaacatc
     6721 ggcaccagta gctaatgcaa ctaaaactat ctgattaacc cctcctggtg ctgcaccaag
     6781 aaacaattca ttaataggat tattatcaaa gaaatgtatg atataaacca tgattagcgc
     6841 accaattatc aacataatat tttgaattgt aattgcgatt gctagtctac cttttaaatc
     6901 tgacaataaa tgcgcaattt gaactccaat tctaatcata tatattagtt gtgccatgtt
     6961 caacaaccaa tgatctagtg taaatgttaa acctgtagaa aaattccaaa caattaatac
     7021 aatgagtggt gctaataatt gaaatgttgg aaactttatt ttagacataa ttagataaac
     7081 tataaagata gctatcgcta aaataactat ttgccctatg tttaatactt gtgataaagg
     7141 caagactttt gttaactttc cattcgcatg catgttacca tcatgaaaaa aatatgaaat
     7201 gaacggtact aaaacaacaa caaatataat tcgtgatgtt tgcgttaagc taacaactaa
     7261 caaattagca cgtttgtctt gttcagccat gaccagcatt tgtgttagtg ctcctggtat
     7321 aacacttaaa atagctgttt ctgtattaat acgtgcaatt tttttaaaaa taaatgccat
     7381 tactattgca attaataata tcgaaataga tacaacaata atcgaaagcc aattgttttt
     7441 aatatccata acgacatttt tcgtaaacgt tgatccgatt tgcaccccta atagtacaat
     7501 ccctaattca ctaagtaaga atggccattt aatatcaagt ttgaaaactt tcacacaaat
     7561 gattgatgcg ataataggac caaacataaa tgggagtaat acgtgcgatg aatacaatat
     7621 aatactaata aaaaatgata aaacgaacac aatgaaatta tttctatata tcattgtcat
     7681 gttttccact tctttcaata aaaaataaaa tgactaaatt gctgcttgag cttcacgttt
     7741 gttaagataa caatatccgc tagcagtttt gactacaaag catatatgga ctttcactat
     7801 caagtcgccg cccatgcctg atatacattt aaaaagagcc tgaacaaagt tcaggctctc
     7861 aatttgtccg tatatttatt ttacaatacg acttaaagcc gtatcaaatg cttgaatcgt
     7921 tttttcaata tcttctttcg tgtgtgccgt agataagaat gtaccttcaa attgagatgg
     7981 tggtaaaaac acaccttctt ttgccatttc tcgatacatt tctgcaaata atttcaaatc
     8041 acttttattc gcttgttcaa aattagttac aggtccttca tttaagaaat aaccaatcat
     8101 tgaacctgct ctatttacag ttattggtac attgtgttta gcaaatacac gctttaaacc
     8161 gtcttcaagt atatcgccta acatattaaa atactcatat gtctctggcg ttaattggct
     8221 taacgtttca taaccacttg tcattgcaag aggatttcct gataacgtac ccgcttgata
     8281 aatatttcct aatggtgcta tatgatccat gatttctttt ttaccaccaa aagcacctac
     8341 aggtagtcct ccaccgataa cttttcctaa gcaagttaaa tctggtgtca caccaaagta
     8401 accttgtgca caatgataac cgactctgaa accagtcatt acttcatcga aaattagcaa
     8461 tgcgccgtat tcagtcgtaa tatctcttaa tccctgtaaa aaaccttcaa tcggtggtac
     8521 gacacccata ttaccagcaa caggttctac gattacacca gcaatatcgt ctccaaattt
     8581 ttcgaaagcg attttaagtg catctaaatc attgtatgga actgtaattg tatttttagc
     8641 aataccttca ggcacaccag gagaatccgg caatcctaat gttgccaccc cagaaccagc
     8701 tttgattaat aacgaatcac tatgaccatg atagcaacct tcaaatttca caattttatt
     8761 tctgccagta taaccacgtg ctaatcttaa agtatccaat gtagcttctg taccagatga
     8821 caccatacgc actttttcta ttgaaggtac tcggtcaata acgagctgcg ccaatttatt
     8881 ttcaagtaat gttgatgcac caaaacttgt acctttatca attgcttcat gtaaatgact
     8941 aataacttga gggtctctat gtcctaaaat aagcggcccc caacttagta catagtcgat
     9001 atactcgtta ccatcgatat cataaatttt tgagccttta ccgtgatcca taaaaattgc
     9061 tggtgtatct actgatttaa atgcgcgtac tggactattt acaccaccag gcattaaagt
     9121 ttcagcaacc ttcattgctt cttctgattt cgtatatctc ataaagtaaa ttcctcctat
     9181 agtttatgga aaatcataca tataaaacct tatttatcta aatagcgaca aatgtccttt
     9241 gcaaaatacg taataatcat atcagcacct gcacgtttca ttgaaaccat ttgttccata
     9301 acgacacgtt cttcatctat ccaaccattt tgtgccgctg ctttagtcat actatattct
     9361 ccactcacat tatatgcaac aactggaaca ttcgtatgat ttttaacatc tcgaactatg
     9421 tctaaataac ttagagcagg tttaacaatc atcatgtcgc acccttcttt aagatcactt
     9481 tctaattcac gaagtgcttc caaacggtta gcagggtcca tctgatacgt ttttctatcc
     9541 ccaaatgatg gcgctgaatc tgctgcatct ctaaaaggtc caaagaaact tgatgcatac
     9601 ttgacaccat aactcattat aggaatattg taatagccgg cttcatctaa tccacgacga
     9661 atttcagcaa caaaaccatc catcatatta cttggcgcaa taatatcagc accagcttcc
     9721 acttgagaaa ttgctgtttt aacaagtagt ggcaatgatt tatcattgtc aacgtcatgt
     9781 gtatggtcat caatcacgcc acaatgacca tgatcagtat attcacataa acaagtgtct
     9841 gcaacaatta ataagtcatc atacattttt ttagcaatac gtgttgcctg ttgaataaca
     9901 ccatcgtgaa tgtatgcacc agtacctata tcatcttttg agtttggaac accgaaaaac
     9961 ataatggcac gtatgcctaa gtcataagct tcttttaatt cactttcaag taaattcaaa
    10021 ctgatttggt atacacctgg caatgactta atttcttttt tcacatcgtc tttttcaact
    10081 acaaaaattg gatatattaa atcttctttt cttacatgat tctctctaac catatctctc
    10141 attgtcgctg atgatctcaa tcttctatgt ctatcaaatt tcatttttag cccctacttt
    10201 ctaaaatctt ttcaattagt gattcgagtg tttgaatttc tgcaattgtt actggttgtt
    10261 gatatgtttt aatggtccgt gctgtttgtt ctccaatagc aaaatacgac ttgaattttg
    10321 gtacaaatcc ttcattaaaa taataacgta ctgccgacga acttgaaaat gttaatgcat
    10381 cgatttgttg atgttctatc atttctttaa catcttgtat attttgtttg ttaggcactg
    10441 aagtatataa atctatttta acaacttcat tatctttaga taacgctgct aataacaatg
    10501 gtctcgccaa ttcactcgaa ggcaaaagta ttttttggtt agtttgatta aatgatttta
    10561 aaaatccttc ttgagaaaag tcgtttggca taaaatcaac tcgaatgcca agtgattcac
    10621 aatattgcgc tgtcttactt cctatcacag caatgttatc aacattaatt ccttttagat
    10681 atttataaaa gaatttcaca gcatttttag atgaaaaaat aagccagtca tagcgttgat
    10741 ttaacaaatg aatatcaaaa tttagtggct ttatatcaat aaagggtttg tgaataattg
    10801 atactaaatc actttgcatg tcatttgttt gtgtcataac tacaactggc ttcatattta
    10861 aacgtcactc cattatttaa tgttgttcat ttaagcgttt tataatttca taagcacctt
    10921 gctcttttaa tttgttactc actgttttgc ctaactcaac cggatctgtt ccgttcattg
    10981 tatattcaaa tcgttcttta ccatctgggg tcataattaa acctgtaaat tcgatttctt
    11041 tttgatctga gattgtagca tatcctgcga ttggcacctg acaactacca tccatttctg
    11101 ctaaaaacgt tcgttcagca gtcacacatt ttgcaacctc atcattatgt actttgctta
    11161 ataatgttaa tagttcttca tcgtcactac gacattctat ccctaaagca ccttgtccga
    11221 ttgcaggtaa caatgtatct ctatcaagat aagatgttac aatatcatct gaccagccca
    11281 ttcttcttaa accagctgca gctaaaataa tcgcatcata atcttcagtt tgtaactttt
    11341 ctaatcgtgt atctatatta cctctaatcc atttaatctc taaattagga tacttagata
    11401 atatttgtgc accacgacgt aatgaactag taccaataat actgccttct ggcaattggg
    11461 atagtggtgt atgtgtttta gaaatatacg catcaaaagg taattctcta tcagggatac
    11521 aacctaatgt taaaccttcc ggaattacac ttggtacgtc tttaagcgag tgtattgcca
    11581 tatcgatatt tttttcaaaa agttcatgtt gtatttcttt aacaaataag cctttgcctc
    11641 cgactttaga caattgttta tctactatac gatcgccttt cgtgacaatt tctttaattt
    11701 caatttctag atttggctcg acagctttta atttattaat aaattgctgg ctttgtgtta
    11761 aagctaattt acttcttctg gagccaacga ctaatttacg catgttcaat tcctcctagg
    11821 aacggattgc tctagattat tttctcaatt cacaaaatgt gttgcaaaaa ataaattaat
    11881 catatttaag caaa
//
LOCUS       DANBWH010000074        11710 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.077, whole genome shotgun sequence.
ACCESSION   DANBWH010000074 DANBWH010000000
VERSION     DANBWH010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11710
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.077"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..243
                     /locus_tag="PPY92_001372"
     CDS             <1..243
                     /locus_tag="PPY92_001372"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625384.1"
                     /translation="SWGEGPNTEADEKSAYNNVQVGDGPQQREIGFPISTDNASWRGP
                     NIEAGGKSVYNNVQVGVGRRNKFCENIISVPLPSKE"
     gene            533..3238
                     /gene="acnA"
                     /locus_tag="PPY92_001373"
     CDS             533..3238
                     /gene="acnA"
                     /locus_tag="PPY92_001373"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HDJ2625385.1"
                     /translation="MAANFKEQSKKHFDLNGQSYTYYDLKAVEEQGITKVSNLPYSIR
                     VLLESLLRQEDDFVITDDHIKALSQFGKDGNEGEVPFKPSRVILQDFTGVPAVVDLAS
                     LRKAMDDVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFL
                     NWATKAFDNYNAVPPATGIVHQVNLEYLASVVHVRDVDGEKTAFPDTLVGTDSHTTMI
                     NGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLVNSLPQGATATDLALRVTQELR
                     KKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDDESLKYMKLTGRSD
                     EHIALVKEYLKQNHMFFDVEKEDPNYTDVIELDLSTVEASLSGPKRPQDLIFLSDMKS
                     SFENSVTAPAGNQGHGLDKSEFDKKAEINFKDGSKATMKTGDIAIAAITSCTNTSNPY
                     VMLGAGLVAKKAVEKGLKVPEYVKTSLAPGSKVVTGYLRDAGLQPYLDDLGFNLVGYG
                     CTTCIGNSGPLLPEIEKAIADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAY
                     ALAGTVDIDLQNEPIGKGNDGEDVYLKDIWPSIKEVSDTVDSVVTPELFIEEYNNVYN
                     NNELWNEIDVTDQPLYDFDPNSTYIQNPSFFQGLSKEPGTIVPLNGLRVMGKFGDSVT
                     TDHISPAGAIGKDTPAGKYLQDHQVPIREFNSYGSRRGNHEVMVRGTFANIRIKNQLA
                     PGTEGGFTTYWPTNEVMPIFDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLG
                     VKTVIAQSYERIHRSNLVMMGVLPLEFKKGESADSLGLDGTEEISVNIDENVQPHDYV
                     KVTAKKQDGDLVEFDAMVRFDSLVEMDYYRHGGILQMVLRNKLAQ"
     gene            3418..3885
                     /locus_tag="PPY92_001374"
     CDS             3418..3885
                     /locus_tag="PPY92_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDJ2625386.1"
                     /translation="MIYSITEIEARYAETDKMGVIYHGNYATWFEVARLDYISKLGFS
                     YADMEKQGIISPVTDLNVNYKKSIFYPEKVKVKTWVEKYSRLRSVYKYEIFNEKGELA
                     TTGSTELICIKEDTFKPIRLDRYFPDWHEAYSKVQTLNNEGKTVEIMDGIDSL"
     gene            complement(4092..4388)
                     /locus_tag="PPY92_001375"
     CDS             complement(4092..4388)
                     /locus_tag="PPY92_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HDJ2625387.1"
                     /translation="MQIELTDAAVTWFKNELELPENNKVLVFFVRYGGEFQLKQGFSP
                     AFTVEPKEDVDIGYEQQYDDLNVVVAEKDLWYFEDDHIIVNVVDHEDEISYSTK"
     gene            complement(4764..5372)
                     /gene="plsY"
                     /locus_tag="PPY92_001376"
     CDS             complement(4764..5372)
                     /gene="plsY"
                     /locus_tag="PPY92_001376"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDJ2625388.1"
                     /translation="MMIIVMLLLSYLIGAFPSGFVIGKLFFKKDIRQFGSGNTGATNS
                     FRVLGRPAGFLVTFLDIFKGFITVFFPLWLPVHADGPISTFFTNGLIVGLFAILGHVY
                     PVYLKFQGGKAVATSAGVVLGVNPILLLILAIIFFIVLKIFKYVSLASIVAAICCVIG
                     SLIIQDYILLVVSFLVSIILIIRHRSNIARIFRGEEPKIKWM"
     gene            5578..7569
                     /gene="parE"
                     /locus_tag="PPY92_001377"
     CDS             5578..7569
                     /gene="parE"
                     /locus_tag="PPY92_001377"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDJ2625389.1"
                     /translation="MNKQNNYSDDSIQVLEGLEAVRKRPGMYIGSTDKRGLHHLVYEI
                     VDNSVDEVLNGYGNEIDVTINKDGSISIEDNGRGMPTGIHKSGKPTVEVIFTVLHAGG
                     KFGQGGYKTSGGLHGVGASVVNALSEWLEVEIHRDGSIYHQSFKNGGSPSSGLVKKGK
                     TKKTGTKVTFKPDDTIFKASTSFNFDVLSERLQESAFLLKNLKITLNDLRSGKERQEH
                     YHYEEGIKEFVSYVNEGKEVLHDVATFSGEANGIEVDVAFQYNDQYSESILSFVNNVR
                     TKDGGTHEVGFKTAMTRVFNDYARRINELKTKDKNLDGNDIREGLTAVVSVRIPEELL
                     QFEGQTKSKLGTSEARSAVDSVVADKLPFYLEEKGQLSKSLVKKAIKAQQAREAARKA
                     REDARSGKKNKRKDTLLSGKLTPAQSKNTEKNELYLVEGDSAGGSAKLGRDRKFQAIL
                     PLRGKVINTEKARLEDIFKNEEINTIIHTIGAGVGTDFKIEDSNYNRVIIMTDADTDG
                     AHIQVLLLTFFFKYMKPLVQAGRVFIALPPLYKLEKGKGKTKRVEYAWTDEELNKLQK
                     ELGKGFTLQRYKGLGEMNPEQLWETTMNPETRTLIRVQVEDEVRSSKRVTTLMGDKVQ
                     PRREWIEKHVEFGMQEDQSILDNSEVQVLENDQFDEEEI"
     gene            7569..9971
                     /gene="parC"
                     /locus_tag="PPY92_001378"
     CDS             7569..9971
                     /gene="parC"
                     /locus_tag="PPY92_001378"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDJ2625390.1"
                     /translation="MSEIIQDLSLEDVLGDRFGRYSKYIIQERALPDVRDGLKPVQRR
                     ILYAMYSSGNTHDKNFRKSAKTVGDVIGQYHPHGDYSVYKAMVRLSQDWKLRHVLIEM
                     HGNNGSIDNDPPAAMRYTEAKLSLLAEELLRDINKETVSFIPNYDDTTLEPMVLPSRF
                     PNLLVNGSTGISAGYATDIPPHNLAEVIQATLKYIDNPDITVNQLMKYIKGPDFPTGG
                     IIQGIDGIKKAYESGKGRIIVRSKVEEETLRNGRKQLIITEIPYEVNKSSLVKRIDEL
                     RADKKVDGIVEVRDETDRTGLRIAIELKKDVNSESIKNYLYKNSDLQISYNFNMVAIS
                     DGRPKLMGIRQIIDSYLNHQIEVVANRTKFELDNAEKRMHIVEGLIKALSILDKVIEL
                     IRSSKNKRDAKENLIEVFEFTEEQAEAIVMLQLYRLTNTDIVALEGEHKELEALIKQL
                     RHILDNHDALLNVIKEELNEIKKKFKSERLSLIEAEIEEIKIDKEVMVPSEEVILSMT
                     RHGYIKRTSIRSFNASGVEDIGLKDGDSLLKHQEVNTQDTVLVFTNKGRYLFIPVHKL
                     ADIRWKELGQHVSQIVPIEEDEVVINVFNEKDFNTDAFYVFATQNGMIKKSTVPLFKT
                     TRFNKPLIATKVKENDDLISVMRFEKDQLITVITNKGMSLTYNTSELSDTGLRAAGVK
                     SINLKAEDFVVMTEGVSENDTILMATQRGSLKRISFKILQVAKRAQRGITLLKELKKN
                     PHRIVAAHVVTGEHSQYTLYSKSNEEHGLINDIHKSEQYTNGSFIVDTDDFGEVIDMY
                     IS"
     gene            10221..11681
                     /locus_tag="PPY92_001379"
     CDS             10221..11681
                     /locus_tag="PPY92_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDJ2625391.1"
                     /translation="MKDFDSLIPGWFKEFVHVGTDLIWSQYLIGLLLTAGFFFTISSK
                     FVQLRMLPEMFRALVERPETLEDGKKGISPFQAFAISAGSRVGTGNIAGVATAIVLGG
                     PGAVFWMWVIAFIGAASAFIEATLAQVYKVHDKDGGFRGGPAYYITKGLNQKWLGIVF
                     AILITITFAFVFNTVQSNTIAESLNTQYNISPVITGIILAIVTAIIIFGGVRSIATLS
                     SLIVPIMAIIYIGMVLVILLFNLDQIVPMIGTIIKSAFGIEQVTGGAVGAAVLQGIKR
                     GLFSNEAGMGSAPNAAATAAVPHPVKQGLIQSLGVFFDTMLVCTATAIMILLYSGLKF
                     GDNAPQGVAVTQSALNEHLGSAGGIFLTIAVTLFAFSSVVGNYYYGQSNIEFLSTNRV
                     ILFIFRCLVVVLVFVGAVVKTETVWNTADLFMGLMAIVNIISIIGLSNVAFALMKDYQ
                     KQKKEGKNPVFKPENLEINLFGISAWGANKYKNSDK"
BASE COUNT         4079 a         1700 c         2263 g         3668 t
ORIGIN      
        1 agttggggtg agggccccaa cacagaagct gacgaaaagt cagcttacaa taatgtgcaa
       61 gttggggatg ggccccaaca aagagaaatt ggattcccaa tttctacaga caatgcaagt
      121 tggcggggcc ccaacataga agctggcgga aagtcagttt acaataatgt gcaagttggg
      181 gtgggacgac gaaataaatt ttgcgaaaat atcatttctg tcccactccc atcaaaagaa
      241 tgacattgaa aagaaatatt aaagtttggc gccacaattt tctttctgct caatgaaata
      301 atacgtttga aaattgaatt gaatcattca cgaaattgat aattattctc aattaataaa
      361 aaatacttat tacaccttac ctgtatgata agttttgctt atatactctg attaaaaagt
      421 caaaacctaa attaagcgtt ttctaagcat gatgagttgt ttatcaataa atataataat
      481 acaatttaat caaggcatat aaatataaaa atgtatcaag ggggatcatt aaatggctgc
      541 aaattttaaa gagcaatcaa aaaaacattt tgacttgaat ggccaaagtt atacttacta
      601 tgatttaaaa gctgtagaag agcaaggtat tactaaagtt tccaatttac cttattcaat
      661 tcgtgttttg ttagaatctt tacttcgtca agaagatgat tttgtaatta cagacgatca
      721 tattaaagct ttaagtcagt ttggaaaaga tggaaatgaa ggcgaggtac catttaaacc
      781 ttctcgtgtt attttacaag atttcacagg tgtaccagcc gtagttgatt tagcttcttt
      841 acgtaaagca atggatgacg ttgggggaga tattactaaa attaatccag aagtaccggt
      901 ggatttagtt attgaccact cagttcaagt ggatagctat gcaaatccag aagctcttga
      961 acgtaatatg aaattagaat ttgaacgtaa ctatgaacgt tatcagtttt taaattgggc
     1021 aacgaaagca tttgataatt acaatgcagt tcctcctgca actggaatag ttcaccaagt
     1081 taacttagaa tatttagcaa gtgttgtaca tgttcgtgat gtagatggtg aaaaaactgc
     1141 atttccagat acattagttg gtactgattc acatacaaca atgataaatg gtattggcgt
     1201 actaggatgg ggtgttggtg gtattgaagc tgaagctgga atgcttggac aaccttctta
     1261 tttcccaatt ccagaggtta ttggtgtacg actagtaaat tcattaccac aaggcgcaac
     1321 agcaactgat ttagcgttaa gagtaactca agagctacgt aaaaaaggtg ttgttggtaa
     1381 atttgtggag ttctttggtc caggtgtaca acatttacca ctagcagacc gtgctacaat
     1441 tgcaaacatg gcaccagagt atggagcaac ttgcggattc ttcccagttg atgatgaatc
     1501 tcttaaatat atgaagttaa ctggtagatc agacgaacat atcgcgctag taaaagaata
     1561 tttgaaacaa aaccatatgt tctttgatgt tgagaaagaa gatcctaatt atacagatgt
     1621 tatcgaattg gatttatcaa cagttgaagc atcgctttca ggaccaaaac gtcctcaaga
     1681 tttaattttc ttaagtgata tgaaatcatc atttgaaaat tctgtaacag ctccagcagg
     1741 caaccaagga cacggtttag ataaaagtga atttgataag aaagctgaaa ttaactttaa
     1801 agatggatca aaagctacaa tgaaaacagg tgatattgca atagcagcaa ttacatcatg
     1861 tacaaataca tctaaccctt atgtaatgtt aggtgcaggt ttagttgcta aaaaagcagt
     1921 tgaaaaaggc ttgaaagttc ctgaatacgt taaaacttct ctagcaccag gatcaaaagt
     1981 tgttaccgga tatttaagag atgctggctt acaaccttat ttagatgatt taggcttcaa
     2041 cttggttggt tatggatgta caacttgtat cggtaattca ggtcctttat taccagaaat
     2101 tgaaaaagcg attgctgatg aggacctatt agtgacatct gtattatctg gtaaccgtaa
     2161 ctttgaaggt cgtatccatc ctcttgttaa agccaattac ctagcttcac cacagttagt
     2221 tgttgcttat gcattagctg gaacggttga tattgattta cagaatgaac ctattggtaa
     2281 aggtaatgac ggtgaagatg tatatttgaa agatatttgg ccatcaatta aagaagtttc
     2341 agataccgtt gatagtgttg taacacctga attatttatt gaagaatata ataacgtata
     2401 caataacaac gaattatgga atgagattga tgtaactgat caacctctat atgactttga
     2461 tcctaattca acatacattc aaaatccatc attcttccaa ggattatcta aagaaccggg
     2521 tacgattgtt ccattaaatg gtttacgtgt tatgggtaaa ttcggtgatt ctgtgacaac
     2581 tgaccacatc tctccagcag gtgcaattgg taaagatacg ccagctggta aatatttaca
     2641 agatcatcaa gtgcctattc gtgaatttaa ttcatatggt tcaagacgtg gtaatcacga
     2701 agtaatggtt cgaggtacgt ttgctaatat acgtattaaa aaccaattag cgccaggtac
     2761 tgaaggtggt tttacaactt attggccaac aaatgaagta atgcctatct ttgatgctgc
     2821 aatgaaatat aaagaagatg gtacaggttt agttgtatta gctggtaacg attatggtat
     2881 gggttcatct cgtgactggg cagcaaaagg tacaaactta ttaggtgtta aaacagttat
     2941 tgcacaaagt tatgaacgta tccatcgttc aaatttagtt atgatgggtg tattaccatt
     3001 agagtttaaa aaaggtgaat cagctgattc tcttggtcta gatggtacag aagaaatttc
     3061 tgttaatatt gatgaaaatg ttcaaccaca tgactacgtc aaagttactg ctaagaagca
     3121 agatggtgat ttggtagaat ttgacgctat ggttcgtttt gactcacttg ttgaaatgga
     3181 ttactatcgt cacggtggaa ttttacaaat ggttttaaga aataaattag cgcaataaaa
     3241 aatagatatc acagtaaaat ttcaatcggt atttgaggca ctttgatagt gctcaaaaat
     3301 acttctataa actttatgat ataataggtc ggttcaaaac ctttgtgttc ttggccggcc
     3361 tcttcttgta ttttatgtta gaatgagata taaagttaaa ctgaaagaag gcttaaaatg
     3421 atatatagta ttacagaaat agaagcgcgt tatgctgaaa ctgataagat gggtgtaatt
     3481 tatcacggga attatgcaac ttggtttgaa gttgcgcggt tggattatat atcgaagtta
     3541 ggttttagtt atgctgatat ggaaaaacaa ggaatcattt cacctgtgac tgacctcaat
     3601 gtcaattata aaaagtctat tttttatcca gaaaaagtta aagttaaaac ttgggttgaa
     3661 aaatattcga gattacgttc agtgtataaa tatgagattt ttaatgaaaa aggtgaactt
     3721 gcaactacag gttccacaga attgatttgc attaaagaag atacttttaa gcctatacgg
     3781 ttggatcgtt atttcccaga ttggcatgaa gcttatagta aagtgcaaac gctcaataat
     3841 gaagggaaaa cagtagagat aatggatggt attgattctt tataagttac tgtaaatgtt
     3901 tggagaggca atattaacaa ttaatctata agaaatagcc aatacgttaa cattaatgta
     3961 attagcgtga agataagcta atatatagca aaaacgaact tgttcataca acatcagatg
     4021 tgctaaacca aagtacaact tccgaggttc aggacacatc agaatgtgta aacaagttcg
     4081 ttttatttta gttattttgt ggaataagaa atttcatctt cgtgatcaac tacatttaca
     4141 ataatgtggt catcttcaaa gtaccacaaa tctttttccg ctacgacaac atttaaatcg
     4201 tcatattgtt gttcatagcc aatatcaaca tcttcctttg gttcaactgt aaaagcagga
     4261 ctaaatcctt gcttgagttg gaattcgcca ccatatctta caaaaaacac gagcacttta
     4321 ttattttcag gcaactcaag ttcattttta aaccaagtta ctgctgcatc agtaagttct
     4381 atttgcataa caatgacctc ctatatttat tacagaatgg gagtgggcca acttgtatta
     4441 tttgtggaat ttcttatcga aattctctgt gttggagccc accccaacct gctcattatt
     4501 gtaagctgaa tttccataag cttctatgtt gggacccccg accagaattg aaaaaaccta
     4561 gttataagcg catttcgttc ggttaactac agctaatata acattgtgga gcctatgaca
     4621 ttgatttatg tctcggtctc aaatgttctt gtaaaaaaac taagtatagt tgaattttaa
     4681 gttaaaaaaa ttgtttttgc aaaaataaaa taatccatca actgcaaaaa aatatgcaat
     4741 gatggactaa tgtcaaaaaa ttgttacatc cattttattt taggttcttc gcctctaaaa
     4801 atccttgcga tattagagcg atgtctaatt atcaatatga ttgaaactaa gaaactaacg
     4861 actaataaaa tatagtcttg aatgataagc gagccaatca cacagcaaat tgctgcaacg
     4921 atacttgcta aagaaacata tttaaaaatc ttcaatacaa taaagaagat aattgcaagt
     4981 attagtaaaa gtatcggatt gactcccaag acgacacctg cactagttgc aacagctttg
     5041 ccaccttgga attttaaata aacaggataa acgtgtccaa gtatagcgaa taagccaaca
     5101 attaaaccat ttgtaaaaaa agtactaata gggccatctg cgtgaactgg taaccataaa
     5161 gggaagaaaa cagttatgaa ccctttgaaa atatctagaa atgttaccaa gaatcctgca
     5221 ggacgaccta atactctaaa gctattagta gcgccagtat taccactacc aaattgtcta
     5281 atatcttttt tgaaaaataa ttttccaatt acgaatccac ttgggaaagc gccgataaga
     5341 taacttagta gtaacatgac gattatcatc ataaatatta cacatccttt aatatcttag
     5401 gactatttta tcatattcat ttaaattacg gctaaaaatt ttaaaaacgg ggattaatat
     5461 atggaattaa gctatgaaag ttaattgata cttgcatttt acgctgattt atataagaat
     5521 aactattgta tagttttaaa aacgaacgta cgtttgcagg aggcgaaatc attggcaatg
     5581 aataaacaaa ataattattc agatgattca atacaggttt tagaggggtt agaagcagtt
     5641 cgtaaaagac ctggtatgta tattggatca actgataaac ggggattaca tcatctagta
     5701 tatgaaattg tcgataactc cgtcgatgaa gtattgaatg gttacggtaa cgaaatagat
     5761 gtaacaatta ataaagatgg tagtatttct atagaagata atggacgtgg tatgccaaca
     5821 ggtatacata aatcaggtaa accgacagtc gaagttatct ttactgtttt acatgcagga
     5881 ggtaaatttg gacaaggcgg ctataaaact tcaggtggtc ttcacggtgt tggtgcttca
     5941 gttgtaaatg cattgagtga atggcttgaa gttgaaatcc atcgagatgg tagtatatat
     6001 catcaaagtt ttaaaaacgg tggttcgcca tcttctggtt tagtgaaaaa aggtaaaact
     6061 aagaaaacag gtaccaaagt aacatttaaa cctgatgaca caatttttaa agcatctaca
     6121 tcatttaatt ttgatgtttt aagcgaacga ctacaagagt ctgcgttctt attgaaaaat
     6181 ttaaaaataa cgcttaatga tttacgcagt ggtaaagagc gtcaagagca ttaccattat
     6241 gaagaaggaa tcaaagagtt tgttagttat gtcaatgaag gaaaagaagt tttgcatgac
     6301 gtggctacat tttcaggtga agcaaatggt atagaggtag acgtagcttt ccaatataat
     6361 gatcaatatt cagaaagtat tttaagtttt gtaaataatg tacgtactaa agatggtggt
     6421 acacatgaag ttggttttaa aacagcaatg acacgtgtat ttaatgatta tgcacgtcgt
     6481 attaatgaac ttaaaacaaa agataaaaac ttagatggta atgatattcg tgaaggttta
     6541 acagctgttg tgtctgttcg tattccagaa gaattattac aatttgaagg acaaacgaaa
     6601 tctaaattgg gtacttctga agctagaagt gctgttgatt cagttgttgc agacaaattg
     6661 ccattctatt tagaagaaaa aggacaattg tctaaatcac ttgtgaaaaa agcgattaaa
     6721 gcacaacaag caagggaagc tgcacgtaaa gctcgtgaag atgctcgttc aggtaagaaa
     6781 aacaagcgta aagacacttt gctatctggt aaattaacac ctgcacaaag taaaaacact
     6841 gaaaaaaatg aattgtattt agtcgaaggt gattctgcgg gaggttcagc aaaacttgga
     6901 cgagaccgca aattccaagc gatattacca ttacgtggta aggtaattaa tacagagaaa
     6961 gcacgtctag aagatatttt taaaaatgaa gaaattaata caattatcca cacaatcggg
     7021 gcaggcgttg gtactgactt taaaattgaa gatagtaatt ataatcgtgt aattattatg
     7081 actgatgctg atactgatgg tgcgcatatt caagtgctat tgttaacatt cttcttcaaa
     7141 tatatgaaac cgcttgttca agcaggtcgt gtatttattg ctttacctcc actttataaa
     7201 ttggaaaaag gtaaaggcaa aacaaagcga gttgaatacg cttggacaga cgaagagctt
     7261 aataaattgc aaaaagaact tggtaaaggc ttcacgttac aacgttacaa aggtttgggt
     7321 gagatgaacc ctgaacaatt atgggaaacg acgatgaacc cagaaacacg aactttaatt
     7381 cgtgtacaag ttgaagatga agtgcgttca tctaaacgtg taacaacatt aatgggtgac
     7441 aaagtacaac ctagacgtga atggattgaa aagcatgttg agtttggtat gcaagaggac
     7501 caaagtattt tagataattc tgaagtacaa gtgcttgaaa atgatcaatt tgatgaggag
     7561 gaaatctagt gagtgaaata attcaagatt tatcacttga agatgtttta ggtgatcgct
     7621 ttggaagata tagtaaatat attattcaag agcgtgcatt gccagatgtt cgtgatggtt
     7681 taaaaccagt acaacgtcgt attttatatg caatgtattc aagtggtaat acacacgata
     7741 aaaatttccg taaaagtgcg aaaacagtcg gtgatgttat tggtcaatat catccacatg
     7801 gagactactc agtgtacaaa gcaatggtcc gtttaagtca agactggaag ttacgacatg
     7861 tcttaataga aatgcatggt aataatggta gtatcgataa tgatccgcca gcggcaatgc
     7921 gttacactga agctaagtta agcttactag ctgaagagtt attacgtgat attaataaag
     7981 agacagtttc tttcattcca aactatgatg atacgacact cgaaccaatg gtattgccat
     8041 caagatttcc taacttacta gtgaatggtt ctacaggtat atctgcaggt tacgcgacag
     8101 atataccacc acataattta gctgaagtga ttcaagcaac acttaaatat attgataatc
     8161 cggatattac agtcaatcaa ttaatgaaat atattaaagg tcctgatttt ccaactggtg
     8221 gtattattca aggtattgat ggtattaaaa aagcttatga atcaggtaaa ggtagaatta
     8281 tagttcgttc taaagttgaa gaagaaactt tacgcaatgg acgtaaacag ttaattatta
     8341 ctgaaattcc atatgaagtg aacaaaagta gcttagtaaa acgtatcgat gaattacgtg
     8401 ctgacaaaaa agtcgatggt atcgttgaag tacgtgatga aactgataga actggtttac
     8461 gaatagcaat tgaattgaaa aaagatgtga acagtgaatc aatcaaaaat tatctttata
     8521 aaaactctga tttacagatt tcatataatt tcaacatggt cgctattagt gatggtcgtc
     8581 caaaattgat gggtattcgt caaattatag atagttattt gaatcatcaa attgaggttg
     8641 ttgcaaatag aacgaagttt gaattagata atgctgaaaa acgtatgcat atcgttgaag
     8701 gtttgattaa agcgttgtca attttagata aagtaattga attgattcgt agctctaaaa
     8761 acaagcgtga cgctaaagaa aaccttatcg aagtattcga gttcacagaa gaacaggctg
     8821 aagcaattgt aatgttacag ttatatcgtt taacaaacac tgacatagtt gcgcttgaag
     8881 gtgaacataa agaacttgaa gcattaatca aacaattacg tcatattctt gataaccatg
     8941 atgcattatt gaatgtcata aaagaagaat tgaatgaaat taaaaagaaa ttcaaatctg
     9001 aacgactgtc tttaattgaa gcagaaattg aagaaattaa aattgacaaa gaagttatgg
     9061 tgcctagtga agaagttatt ttaagtatga cacgtcatgg atatattaaa cgtacttcta
     9121 ttcgtagctt taatgctagc ggtgttgagg atattggttt aaaagatggt gacagtttac
     9181 ttaaacatca agaagtaaat acgcaagata ccgtactagt atttacaaat aaaggtcgtt
     9241 atctatttat accggttcat aaattagcag atattcgttg gaaagaattg gggcaacatg
     9301 tatcacaaat agttcctatc gaagaagatg aagtggttat taatgtcttt aatgaaaagg
     9361 actttaatac agatgcattt tatgtttttg cgactcaaaa tggcatgatt aagaaaagta
     9421 cagtgcctct atttaaaaca acgcgtttta ataaaccttt aattgctact aaagttaaag
     9481 aaaatgatga tttgattagt gttatgcgct ttgaaaaaga tcaattaatt accgtcatta
     9541 ctaataaagg tatgtcatta acgtataata caagtgaact atcagatacc ggattaaggg
     9601 cagctggtgt taaatcaata aatcttaaag ctgaagattt cgttgttatg acagaaggtg
     9661 tttctgaaaa tgatactata ttgatggcca cacaacgcgg ctcgttaaaa cgtattagtt
     9721 ttaaaatctt acaagttgct aaaagagcac aacgtggaat aactttatta aaagaattaa
     9781 agaaaaatcc acatcgtatt gtagctgcac atgtagtgac aggtgaacat agtcaatata
     9841 cattatattc aaaatcaaat gaagaacatg gtttaattaa tgatattcat aaatctgaac
     9901 aatatacaaa tggctcattc attgtagata cagatgattt tggtgaagta atagacatgt
     9961 atattagcta aaaactatat gcaatcacga aattaaatga taaaatacag taatgttaaa
    10021 ttttgactaa attcaaggga tttatattaa atgctgacca agtacttatc gttaaattag
    10081 cgatagactt tgaccaaact aatataaatc tcttgtattg tataagaaat gatgatgtat
    10141 ctctatattt actgaagttt caattcggtc taattttata gaaggtgcat gattcatgta
    10201 gaggatgcga ggggtttatt ttgaaagatt tcgatagttt aattcctgga tggtttaaag
    10261 aatttgtcca tgttggtacc gatttaatat ggtctcaata tttaattggt ctattattga
    10321 cagctggatt cttctttaca attagttcta aattcgttca attacgaatg ttacctgaaa
    10381 tgtttagagc tttagtagaa cgtccagaaa ctttagaaga tggtaagaag ggtatttcgc
    10441 cattccaagc atttgcgatt agtgctggtt cgagagttgg tactggtaat attgctggtg
    10501 ttgcgactgc gattgtttta ggcggtccag gtgcagtgtt ttggatgtgg gttattgcat
    10561 ttataggtgc agcgagtgca tttatagaag cgactttggc tcaggtttat aaagtacatg
    10621 ataaagatgg tggattccgt ggtggtccag cttactatat tactaaaggt ttaaatcaaa
    10681 aatggctagg tatcgtattt gcgattttaa ttacaattac atttgcattt gtatttaaca
    10741 cagtgcaatc taatacaatt gcggagtcgt taaatacgca atataatatt agtccagtaa
    10801 tcacaggtat tattttagca atcgtaacag ctattattat atttggtggt gtacgtagta
    10861 ttgctacgtt atcttcgtta attgtaccga ttatggctat catttacatt ggtatggttt
    10921 tagtaatatt gctatttaat ttagatcaaa ttgttcctat gataggtacg attattaaaa
    10981 gtgcatttgg tatcgaacaa gtaactggtg gcgctgtagg tgctgcggtt cttcaaggta
    11041 tcaaacgtgg tttattctct aacgaagctg gtatgggttc tgcgccgaat gcagcggcaa
    11101 ctgctgccgt accacaccct gttaagcaag gtttaatcca atcattaggt gtgttctttg
    11161 atacaatgtt ggtttgtaca gcaactgcaa tcatgatttt actatattca ggattgaaat
    11221 ttggtgataa cgcacctcaa ggtgttgcag ttactcaatc agcacttaat gagcatttag
    11281 gttctgctgg aggtattttc ttaacaatag cagttacact gtttgcattt tcatctgttg
    11341 taggtaatta ctattacggt caatctaata ttgaattttt atcaacaaat cgtgtgatat
    11401 tatttatctt tagatgtctt gttgtagtac ttgtctttgt cggtgcagtt gtaaaaacag
    11461 aaacagtatg gaatacggca gacttattta tgggcttaat ggcaattgta aacattattt
    11521 ccattatagg actgtccaat gtagcttttg cattgatgaa agattatcaa aagcagaaaa
    11581 aagaaggcaa gaaccctgtc tttaaacctg aaaacttaga aattaactta tttggaatta
    11641 gtgcttgggg cgctaacaaa tataagaact ctgataaata aatattttta atgtaacgat
    11701 tcattatata
//
LOCUS       DANBWH010000075        11641 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.078, whole genome shotgun sequence.
ACCESSION   DANBWH010000075 DANBWH010000000
VERSION     DANBWH010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11641
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.078"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(235..1593)
                     /locus_tag="PPY92_001380"
     CDS             complement(235..1593)
                     /locus_tag="PPY92_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease"
                     /protein_id="HDJ2625392.1"
                     /translation="MFNEIWPLISVVLGIVILLVLIIGFKLNTFISLIITSMITALML
                     GIPLTKIMETIEKGMGSTLGHIALIFGLGAILGKLLADGGGATRIADTLIQKFGQKHV
                     QWAMLVAAFIVGIALFFEVGLVLLIPLVFTVAKRANVSVLKLGLPMVTALSVTHGFLP
                     PHPGPVVIAKELKANVGDVLLYGMIIAIPVTLIAGPIFNKVAQKMIPSAYTREGDISA
                     LGAQKEFTDQEMPGFGMSLLTATLPVILMLVSTITQLVTGHDKPTNLFESIIYMIGTA
                     GTAMLIAVLFAIVTMGLMRKRKMNHIMESVTNAIYPIGMMLLIIGGGGTFKQVLIDGG
                     VGNTIAKMFEGTEMSPILLAWIVAAVLRIALGSATVAAISTTGIVLPLLQSSDVNVAL
                     VVLAIGAGSVILSHVNDAGFWMFKEYFGLTVKETFLTWSLLETIISVSGIIFILFISL
                     FV"
     gene            complement(1710..3263)
                     /gene="gntK"
                     /locus_tag="PPY92_001381"
     CDS             complement(1710..3263)
                     /gene="gntK"
                     /locus_tag="PPY92_001381"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDJ2625393.1"
                     /translation="MKYMIGVDIGTTSTKSVLYDENGAFIMKHQIGYDLHTPNVDVSE
                     ENPDELFDAVLMTIKYIMRESKVNQDDIKFVSFSAQMHSLIAMDQQHQRLTNNITWAD
                     NRAAKYATVINEVHDGNAIYQRTGTPIHPMSPLVKIFWMKHEWQDVFQRTAKFADIKT
                     YIFYHLFDTYIIDYSMASATGMFNLETLDWDVGALELLGISKEMFPELVPTTYVMKGM
                     KERYATLMGLNEDTPFVIGASDGVLSNLGVNSVGKGEVAVTIGTSGAIRTVIDKPRTD
                     YKGRIFCYVLTEDHYVIGGPVNNGGVVLRWLRDELLASEVETAKRLGVDPYDVLTQIA
                     KRVKPGADGLIFHPYLAGERAPLWNANARGSFFGLTLSHKKEHMIRAALEGVLYNLYT
                     VYLALIEVMNETPNMIKATGGFAKSEVWRQMMSDIFDTELVVPESYESSCLGACVLGL
                     KAVGDIEDFSIVSSMVGATNNHTPFEENVAVYQELVSIFINLSRSLTENYEQIADFQR
                     QHMAENKTQ"
     gene            complement(3287..3967)
                     /locus_tag="PPY92_001382"
     CDS             complement(3287..3967)
                     /locus_tag="PPY92_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDJ2625394.1"
                     /translation="MMYGYPEKWLEGMTTGEGIAAELRLGIVNGHIAEGTLLTENQMA
                     KQFNVSRSPIRDAFKLLQQNQLIQLERMGAHVLPFGEQEKKEMYDLRLMLESFAFSRV
                     KNQERLPIVKEMKKQLEMMKVAVKFEDAESFTKHDFEFHETLIKASNHQYLNSFWSHL
                     KPVMMALVLTSMRQRMQQNPQDFERIHHNHQVFIDAVEQYDSQILKEAFHLNFDDVGK
                     DIEGFWLN"
     gene            complement(4120..4884)
                     /locus_tag="PPY92_001383"
     CDS             complement(4120..4884)
                     /locus_tag="PPY92_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDJ2625395.1"
                     /translation="MSNYSTGELAKLCNVTTRTIQYYDRKGILKPQGFTEGKRRVYTE
                     QQRQTLELILLLKDLGCALSDIDMLLKGEGTLKTLNTLLTMKQQEINQQVKQQQAVLN
                     KIKNVQYYVNEASTSPITHLKDIEHVMSKSAEMKSIRRNIWISAGIIGIIQYSSIMSS
                     ILMKNKWPFLIALPFMNCYGIGVTFYYQQKVAYLCPNCQHIFSPSLWAVIKAKHTATT
                     RRFECPNCHETHYCIEVPKAHMSTEQLEISHIQHNN"
     gene            complement(5049..5741)
                     /locus_tag="PPY92_001384"
     CDS             complement(5049..5741)
                     /locus_tag="PPY92_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDJ2625396.1"
                     /translation="MYVDRKPSLYLEDLRHDFKNSLSKFENGDEAFDTLLGFVELDHI
                     YSSALKEISTKLSILDDNFNHIYKHNPIHHMERRVKEMRSLIEKLNRKGLQISAETAK
                     EHILDIAGIRVVCNYLDDIYLIEEMLLKQEDVQLIKRKDYIQHPKENGYRSLHIVVSI
                     PVFLAERVEVLPVEIQIRTIGMDMWASLEHKIRYKNNAETEKYRDLLKECATEITEVE
                     DKLQQIHSEITE"
     gene            complement(5972..6388)
                     /locus_tag="PPY92_001385"
     CDS             complement(5972..6388)
                     /locus_tag="PPY92_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDJ2625397.1"
                     /translation="MPFTMKGYPEKWKEFEVIKRQKAIEIANAMLKEGYLEKDVVPIA
                     AKKAKDWYRALSKEEIKALEEENFVQFHTATMEKAEVDSEYATTDNEEHIQEQSNQDK
                     SYFDSESIGNQSGGVRQREPLMDGAHPKVVESFKDV"
     gene            complement(6527..8350)
                     /locus_tag="PPY92_001386"
     CDS             complement(6527..8350)
                     /locus_tag="PPY92_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDJ2625398.1"
                     /translation="MIIPAIIGYLCGNFQFGLLVATGTLAHIYVFKGPSRSKLRTVII
                     CNLAFAICMMLGTLTAKTPLVFGMTLLIVTVIPFYIFTALKIAGPSSTFFIVTFSLPI
                     NLPIAPEEALYRGFAILVGGILATMMVLITIVFSKNKAEEQAIQNDFKLISKLLHTYN
                     DKAAFLQVAKTAVDSFKASDKLLITSTSSNDKLSRRFQKLLLLHTSAQGIYSELLELN
                     AKQIRPLPDELIEMMDHIIAQLDNSDENVRYWRKEVTVTEEFQNLFNHILKIDEMVHA
                     NEARIAYEADMRKPLYSKRIYQNLTLDSIVFRNTLRYTAIMMIAIFIALMFDFEKAYW
                     IPLSAHTILLGTSTIHAIERGMARGLGTILGVLVLSVILLFSIPTPVAVILMGIAALF
                     TEALVGANYAIAVVFITIQVILMNGLASQNLTINIAFPRVIDVAIGIVIAIIGLFVLG
                     QRTASVLLPNVMAEVVRKEATLFHYLFSENQYKDNVYQKNTAMNLSVKLNNMTQVYNA
                     ANGELFSDKTLIQNYYPSLFALEEISFMLNRAMANEDRLTINEQLMGEYLATFENIAK
                     HLELNTELEIKILPDLPQYNYIQSAMMNIQHNGFRERDKNV"
     gene            complement(8827..10104)
                     /locus_tag="PPY92_001387"
     CDS             complement(8827..10104)
                     /locus_tag="PPY92_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDJ2625399.1"
                     /translation="MKEKRTNVRWMFALAFFFIGVIAYMDRANISYIAKQMMDDLGMT
                     KPQFGLLASFFSLGYALMQVPSGMLAEKFGPRKMITIALVWWSAFTILTGMIKNHGLI
                     YLVRFLFGVGEAPMYPSNAVFNSFWFSKNEKGRASSALLAGSYFGPVLAPIVTIAIVN
                     AFNWQAVFYIFGAVGILMAVLWAIIAKDLPEQHRMVNEAEKRFIMENRDIVATEKSSP
                     PWNNFFKRFSFYAIAIQYFVVQFIITLFLIWLPTYLTEVFHVNFKEMSISSLPWLLMF
                     FLILSAGAISDRVLGLGRSKFVARGVIAIAGFIVFAVSIIFAVRTGNLYVSIFWLSLG
                     LGGIGISMGMSWAAATDLGRNFSGTVSGWMNLWGNIGALISPLLAGLFVEHLGWTMTF
                     QLLIVPAVIAVIMWFYVKPDQPLIVSDDKAIEK"
     gene            10651..11262
                     /locus_tag="PPY92_001388"
     CDS             10651..11262
                     /locus_tag="PPY92_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDJ2625400.1"
                     /translation="MEQIITEFISRFGYAAIFILILLENVLPIVPSEIILTFAGLMSV
                     KSHLSILTLFIIATIASFIGLLILYYICRLISEERLYRFIDRHGKWIKLKSKDLRRAN
                     DWFKKYGVWAVFICRFIPVLRVLITIPAGVNRMNVVTFTVISLIGTTIWNFGLILLGR
                     TLSDSFGMLMTGLHTYSRIMYVVIIIAVIYFAIRYIGKRKRVK"
     gene            complement(11271..11370)
                     /locus_tag="PPY92_001389"
                     /pseudo
     CDS             complement(11271..11370)
                     /locus_tag="PPY92_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501275.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3681 a         2258 c         1692 g         4010 t
ORIGIN      
        1 aaattaaatt catcattatc taatttaaaa caatatacta agtgtttcat aattatcgcc
       61 tgtacaatac gcacaaaaac atgtcttgaa acgcctttca ttacactaaa atccccaata
      121 tactttttat atcgttcgga ttctgactat ttcagacgat tttctgcata aaaataaacg
      181 tgtttcaagg caatatattg caattaccta aaaacacgtt tacttaatat ttagttaaac
      241 aaataagctg atgaatagaa tgaagatgat acctgaaacg gaaataatcg tttctaataa
      301 tgaccatgtt aagaatgttt cttttacagt taaaccaaaa tattctttaa acatccaaaa
      361 tcctgcgtca tttacatgag acaaaatcac actacctgca cctatcgcaa gtacaactaa
      421 tgcaacattt acatctgatg attgtaataa tggtaagaca atacctgtag ttgaaatcgc
      481 agctactgta gccgaaccta atgcgatacg tagcacagct gcaacaatcc atgctagtaa
      541 aattggagac atctctgtac cttcaaacat tttagcaatt gtatttccga caccaccgtc
      601 aattaatact tgtttaaatg taccgccacc gccaataatc aataacatca ttccgattgg
      661 ataaatcgca ttcgtcactg attccataat atgattcatc ttacgttttc tcattaatcc
      721 catcgtaacg attgcaaata atactgctat tagcatggct gtccctgctg ttcctatcat
      781 ataaatgata gattcaaata gatttgtagg tttgtcgtgc ccagttacaa gttgcgttat
      841 cgtagacact aacattaata tgactggtaa tgttgcagtt aataaactca taccaaatcc
      901 tggcatctct tgatccgtaa attctttttg tgcacctaac gctgaaatat cgccttctcg
      961 tgtatacgca gacggaatca ttttttgtgc aactttgtta aatataggtc ctgcaatgag
     1021 tgtaactgga atggcaataa tcataccgta cagtaataca tctccaacat ttgcctttaa
     1081 ttcttttgcg atgactaccg gtcctggatg tggtggtaaa aagccatgtg tcacagataa
     1141 agctgttacc ataggtagtc ctagttttaa cactgaaaca tttgcgcgtt ttgctactgt
     1201 aaatactaat ggaatcagta agactaaacc tacttcaaag aacaatgcaa taccgacgat
     1261 aaatgctgca acaagcattg cccattgtac atgtttttga ccaaattttt gaatcaacgt
     1321 gtctgcaatt cgagttgcac caccaccatc agcaagtaat ttcccaagta tggcacctaa
     1381 accgaatatt agtgcaatgt ggccgagcgt actgcccatc cctttctcaa tcgtctccat
     1441 aattttagtc aatggtatac ctagcattaa cgctgtaatc atcgatgtga taattaatga
     1501 aataaatgta tttaatttaa acccaataat taatactaat aaaataacga tacctaaaac
     1561 aacactgatt aacggccata tttcgttaaa catgacattc ccctctttct cttttcaata
     1621 gaatgtaaca ccgtcgtcgc gcacgtattc cagataatat atagtagtca tggcacaaca
     1681 actttatcta agatgcttga gtgacgtatt tattgtgttt tattttcagc catatgttgg
     1741 cgttgaaaat ctgcaatttg ttcataattc tctgttaaag aacgacttaa attgataaaa
     1801 atggatacga gctcttggta aacagcgaca ttttcttcaa acggcgtatg attatttgta
     1861 gcaccgacca tcgatgaaac gattgaaaaa tcttcaatgt cacctacagc tttaagtcca
     1921 agcacgcagg cacctaagca tgaactttca taactttcag gaaccactaa ctctgtgtca
     1981 aatatatctg acatcatttg acgccatact tcacttttcg caaaaccacc tgttgctttt
     2041 atcatattag gcgtttcatt cattacttca atgagtgcaa ggtagacggt atacaaattg
     2101 taaagaacac cttctaatgc agcgcgaatc atatgttctt ttttatgaga taaagttaaa
     2161 ccgaagaatg aacctcttgc atttgcgttc caaagcggcg cacgttctcc agctaaatag
     2221 ggatggaata ttaaaccatc tgcacctggt ttaacacgct ttgcaatttg agttaagaca
     2281 tcataaggat caacaccgag acgtttcgca gtttcgactt cactcgctag caactcgtcg
     2341 cgcaaccatc tcaatacgac accaccatta tttacaggac ctccgatgac gtagtggtcc
     2401 tctgttaaga cataacaaaa tattctacct ttgtaatcag tacgtggttt atctatcaca
     2461 gtacgaatcg cgccagatgt accgattgtg acagcaactt ctcctttacc aacactattg
     2521 acacccaaat tagaaaggac cccatcactc gcaccaataa caaacggtgt atcttcatta
     2581 agccccatta atgttgcata acgttctttc atgcctttca ttacgtacgt tgttggcact
     2641 aattccggga acatttcctt ggaaatacca agcaattcta atgccccaac atcccaatct
     2701 aatgtttcta aattaaacat ccctgttgcg gaagccattg aataatcaat gatatatgta
     2761 tcaaataaat gatagaaaat gtatgtttta atatctgcaa acttagcagt acgttgaaat
     2821 acatcttgcc attcatgttt catccaaaaa attttcacta aaggcgacat aggatgaata
     2881 ggcgtacctg ttcgctgata aatcgcattg ccatcatgca cttcatttat tactgttgca
     2941 tattttgcag cgcggttatc tgcccaagta atattatttg ttaatctttg atgttgctga
     3001 tccatcgcaa tcaagctatg catttgcgca ctaaatgaca caaatttaat atcatcttga
     3061 ttaactttcg attccctcat tatgtattta attgtcatta ataccgcatc aaataattca
     3121 tctgggtttt cttctgagac atcaacgttt ggtgtgtgta aatcatagcc gatttgatgt
     3181 ttcatgataa aagctccatt ttcatcatat aagactgact tggtactcgt cgttccaatg
     3241 tcgacaccaa tcatatattt catgataaat ccttctttct ttcattttaa ttcaaccaaa
     3301 atccttcaat atctttacca acatcgtcaa aatttaaatg aaacgcttcc ttcaaaattt
     3361 gactgtcgta ttgttccact gcatcaataa acacttgatg attatgatgt atgcgttcaa
     3421 aatcttgcgg gttctgttgc atacgttgtc gcatggatgt taaaacgagt gccatcatta
     3481 ctggttttaa atgactccaa aatgagttta aatattggtg gttagatgct ttgattaatg
     3541 tttcatgaaa ttcaaagtca tgcttcgtaa atgattctgc atcttcaaat tttactgcca
     3601 ctttcatcat ttcaagttgt ttcttcattt cttttacgat aggtagtctc tcttgatttt
     3661 taactcttga aaatgcaaat gactctagca tcagtcgcaa atcgtacatt tctttctttt
     3721 cttgttcccc aaacggcaac acatgtgcac ccattctttc taattggatg agttgatttt
     3781 gttgcaataa tttaaatgca tctcgaattg gcgaacgact cacattaaat tgctttgcca
     3841 tttgattttc agtgagtaac gtaccttcag ctatgtgacc attcacaata cctaagcgta
     3901 attctgccgc gataccttct ccagttgtca taccttccaa ccatttctct ggatatccat
     3961 acatcatcaa agcactcctt cattacatga catacttgta tacaagtatg ttaatatagt
     4021 tcttaagtgt ttgcaagcgc tttctttatg cgaactaaaa tagtgaccac ccctcttcga
     4081 tgtaaattta aagggaatga tcactatcac acgaatgatt taattgttat gttgtatgtg
     4141 ggatatttct aattgttctg tactcatatg cgctttaggt acttcaatgc aataatgcgt
     4201 ttcatgacag ttcggacatt cgaatcgacg tgttgtcgct gtatgtttcg ctttgataac
     4261 tgcccacaaa gatggtgaga atatatgctg gcagttagga cataaatagg caaccttttg
     4321 ttggtaataa aaagtaacac caatgccgta acaattcata aatggtaaag caattaaaaa
     4381 cggccatttg tttttcatca agattgaact cataatgcta gaatattgaa ttattcctat
     4441 aataccagca ctaatccaaa tgttacgacg aatacttttc atttcagctg atttactcat
     4501 gacatgctct atgtctttta agtgtgtgat tggagacgtc gacgcttcat ttacgtaata
     4561 ttgaacattt ttaattttgt ttaataccgc ttgttgctgt ttgacttgtt ggttaatttc
     4621 ttgttgtttc atggttagta aggtattgag tgtcttcaaa gtaccttcac cttttagcaa
     4681 catatctata tcgcttaacg cacaacctaa atctttaagc aataagatta actctaatgt
     4741 ttgtcgctgt tgttctgtat acacacgacg ctttccttct gtaaatcctt gtggtttcaa
     4801 aatacctttg cgatcataat attgaatcgt tcgtgttgtc acattgcata attttgcgag
     4861 ttctccagtc gaatagttag acatagattc cacctcctac aatcaccata gttgatgacc
     4921 cgacgtcacg agcaagtaca atttccatat ttttaagaaa tttattatac ttggcgtctt
     4981 atttttatga tttcgtacca tgttgattta caaaatcact caaattaagc aacactccta
     5041 ctaaacgtct actctgttat ttcagaatga atttgttgta atttatcttc aacttcagta
     5101 atctctgtcg cacattcttt cagtaaatct cgatactttt ccgtctctgc attgttttta
     5161 taacgtattt tatgttctaa acttgcccac atatccatac ctatcgttct aatttgaatt
     5221 tcaacaggca atacctcaac gcgttctgct aaaaagactg gaatggatac aacgatatgt
     5281 aaactgcggt aaccattttc tttagggtgc tgaatataat ctttacgttt tatcaattgt
     5341 acgtcttctt gtttaagcaa catctcttca atcaaataaa tatcatctaa gtaattacat
     5401 actacgcgaa ttcccgcaat atccagtatg tgttctttgg cagtttctgc actaatctgt
     5461 aatcctttac gattaagctt ttctattaaa ctacgcattt ctttcacacg tcgctccata
     5521 tgatgtatag gattgtgttt ataaatgtga ttgaaattgt catctaaaat actcagttta
     5581 gtgcttattt cctttagtgc tgacgaataa atatgatcta actctacgaa acctaataac
     5641 gtatcaaatg cttcatcacc attttcaaat ttacttaaac tatttttaaa atcatgtcgc
     5701 aaatcctcta aatatagtga tggttttcga tctacataca tttttatact aacctccgat
     5761 atataatcat ctttattgta cctaacattt tattaagata atactaatac actatcgaac
     5821 tttgggctga tactgaaaca gcacaaagaa actcaaacaa tccgaattat gtatcaaaac
     5881 cttcaattac aggaagtaat tttaaaatga tttaacgcaa aaaaacacct gttaccgtta
     5941 tataggtaca agtgcttaat tgatagagat gttatacgtc tttaaatgat tccacaactt
     6001 ttggatgtgc accatccata agcggttctc tttgtcgaac gccaccactt tggttgccaa
     6061 ttgattcgct atcaaagtac gatttatctt gatttgattg ttcttgaata tgttcttcat
     6121 tatcagttgt tgcatattca ctatccacct ctgctttttc cattgttgct gtatggaatt
     6181 gtacgaagtt ctcttcttct aatgctttga tttcttcttt cgataacgct cgataccaat
     6241 ctttcgcttt tttcgccgca ataggaacaa catctttttc aagatatcct tcttttaaca
     6301 ttgcattcgc aatttctatc gctttttgcc gtttaattac ttcaaattct ttccattttt
     6361 cggggtaacc tttcattgta aagggcattt ccttaacctc caatatgtta taattcatgt
     6421 tatataccac cataattata aaatgaaaca tattaaacac aattttttag actcccgtca
     6481 taataacttc agacaaacgt tagtctgacc tgataaatat tttattttaa acgttcttgt
     6541 ctctctcacg aaaaccattg tgttgaatat tcatcattgc tgattgaata taattatact
     6601 gtggtaaatc tggtaatatc tttatttcta attctgtatt taattctaaa tgcttagcga
     6661 tgttttcaaa tgttgctaaa tactcgccca ttaattgttc atttattgta agtctatctt
     6721 cattagccat ggctctattt aacataaagc taatttcttc taatgcgaat aagctaggat
     6781 aataattttg aatcaatgtc ttatcactaa ataattcacc attagctgca ttataaactt
     6841 gcgtcatgtt atttaatttc acagataggt tcatggccgt gtttttctga tacacattat
     6901 ctttatattg gttttcagaa aacaaataat ggaagagcgt tgcttcttta cgaacaactt
     6961 cagccattac attaggaagc aatacggatg cggtacgttg tccaaggaca aataaaccta
     7021 tgattgcaat cacaatacct attgcaacgt caataactct tggaaacgca atgttaattg
     7081 ttaaattctg tgatgctaat ccattcatta aaataacttg tattgtaata aaaactactg
     7141 caatcgcata atttgctccc accaatgctt cagtaaacaa tgctgcaatg cccattaaaa
     7201 ttactgcaac aggtgttggt attgaaaaca acaatatgac tgaaagtact aacacaccta
     7261 aaatagtacc taaacctcgt gccatacctc tctcgattgc atgtatagtt gatgttccta
     7321 gtaatattgt atgtgcagat aacggtatcc agtatgcttt ttcaaaatca aacattaacg
     7381 caataaatat cgctatcatc ataatcgctg tatatctcaa tgtatttcta aaaacaatag
     7441 agtctaatgt taaattttga taaatgcgtt tactatataa aggttttcgc atgtctgctt
     7501 catacgcaat acgcgcttca tttgcatgca ccatttcatc aattttcaat atatggttga
     7561 ataaattttg aaattcctct gttactgtca cttcttttcg ccaatatctt acattttcat
     7621 cactattatc tagttgtgca atgatatgat ccatcatttc aattaactca tctggcaatg
     7681 gtcgaatttg tttagcgttc aactctaaca gttcagaata aatcccttgg gcagatgtgt
     7741 gtaataataa taatttttgg aaacgtctac ttaatttatc gttacttgaa gtagaagtga
     7801 ttaataattt atcagatgct ttaaaactat ccaccgctgt ttttgctact tgtaaaaaag
     7861 cagctttatc attataagtg tgtaacaact tagatatgag tttaaaatca ttttgaattg
     7921 cttgttcttc agctttgttt ttagaaaata cgatcgtgat taacaccatc atagtggcaa
     7981 gtataccgcc tactaaaatc gcaaagcctc tatataatgc ttcttcggga gctataggta
     8041 agttaatggg tagactgaat gtcacaatga agaatgtcga tgacggtcca gctattttta
     8101 aggcagtaaa tatataaaat ggtataaccg taacaattaa taatgtcatt ccaaaaacga
     8161 gtggcgtttt ggctgttagc gtaccaagca tcatacatat tgcaaacgct aaattacaaa
     8221 ttattacagt tcgcagctta gatcgcgacg gacctttaaa aacataaata tgggctagtg
     8281 ttccggttgc aactaataat ccaaattgga aattaccaca taagtaaccg attattgcag
     8341 gaataatcat taacagtccc tgtcttacac ctttataaaa gtctaagtta agtcgattga
     8401 tttttataag tgatgtcaaa aagatgtcat ttcgacttct cctttccttg tcaaaatatt
     8461 tccataatct ttttattagc atagttaaaa tgaatttgtt gctataaatt ttaattacgc
     8521 aacatatgtg attataacac cttatgctca attatctaaa tttaaaaact tttaaagtcc
     8581 aattaaaatt aatttctaat gttatttaac atcgttgaac ctttggtcgc ccacctttgg
     8641 gctagttaaa atttatatgt acttttacaa gcgttacctg actaatgttt taaaacttta
     8701 tttctttaca tatatcattt taataacttc ccgttcatct taaaccatat ctgttttaaa
     8761 aagtttcaat ttcaacgtaa aaaagcaata agccccaaca atgtgaaagc ttactgcttg
     8821 tttaaattat ttttctattg ctttatcatc actaacaatt aaaggttgat ctggtttcac
     8881 atagaaccac ataatcacag cgattactgc tggaacgatt aacaattgga atgtcattgt
     8941 ccaacctaaa tgttctacga atagacctgc taatagcgga ctgattaatg cacctatatt
     9001 accccataag ttcatccacc ctgatactgt accagagaag ttacgtccta agtcagttgc
     9061 tgcagcccaa ctcataccca ttgaaatacc gataccacca agacctagtg ataaccagaa
     9121 aatacttaca tataaatttc ctgtacgtac agcaaagata attgaaactg caaacacaat
     9181 aaatcctgcg attgcaatta cacctctagc tacgaatttt gaacgaccta atcctaatac
     9241 acggtcagaa attgcacctg ctgataagat taagaagaac attaataacc aaggtaatga
     9301 actaatgctc atttctttaa agttaacgtg gaatacttct gttaaatacg tcggtaacca
     9361 aattaagaat aatgtaatga taaattgtac aacaaagtat tgaattgcaa ttgcatagaa
     9421 gctaaaacgt ttaaagaaat tattccatgg tggtgatgac ttttcagtag ctacgatatc
     9481 acgattttcc ataatgaaac gtttctccgc ttcattaacc attctatgtt gctcaggtaa
     9541 gtctttggca ataatcgccc ataatacagc cattaaaata cctactgcac caaaaatgta
     9601 aaatactgct tgccagttaa atgcgttaac aatagcaatt gtaactattg gtgctaatac
     9661 aggtccgaaa tatgatcctg ctaataatgc acttgatgct ctaccttttt catttttaga
     9721 gaaccaaaat gagttaaaca cagcattaga aggatacatt ggcgcctcac ccacaccaaa
     9781 taagaatctc actaaataaa ttaaaccgtg gttcttaatc atacccgtta agattgtaaa
     9841 tgcactccac caaactaatg caattgtaat catcttacgt ggaccgaatt tttcagccaa
     9901 catccccgat ggtacttgca ttaatgcata acctagagag aagaatgatg ccaataaacc
     9961 aaattgtggt ttggtcatcc ctaaatcatc catcatttgt ttagcgatat atgaaatgtt
    10021 tgctctatcc atatacgcaa taaccccaat aaagaagaac gcaagcgcga acatccacct
    10081 tacgttagtt cgtttttcct tcataactat tcctacttcc ctaattaatt ttttaaaact
    10141 taccctgaaa cgttaaaatt ttcggacatc tggcatgtcc acccctactt tgtcacaaaa
    10201 ttgtaccaaa gttatttttt ataatattat cagacatgga aagcgtttac aatagaatct
    10261 ataaaaattt tattaaattc cgcttaactt ttgctattaa aacgatataa aggaactttt
    10321 agttacatag tagcctatca tttgttaatt aaaaatactg caatcctagt gttttaaaac
    10381 gtatattgca ggctttctta catattcgac attcatttta aattgcataa aactacattg
    10441 ctcattcatt gtgcagcgcc tgttatttag atgatcattt gttgactttt cgtacttata
    10501 gcaagcaatt ttttgattta ttgcttataa tacattttaa gacaaatgct tgtttcaaca
    10561 tcgcatacga catcattcat tattcatttg aatttatcca gtttctattt gtctgcaatt
    10621 gctgaaaatt aaaggagctt atttttaata atggaacaaa ttatcactga atttattagt
    10681 cgttttgggt atgcagccat ttttatatta attttattag aaaatgtttt acctatcgta
    10741 ccatcggaaa ttattctgac atttgctggc cttatgtctg ttaaatcaca tttatcaatt
    10801 ttaaccttat ttattattgc aaccatcgca tcgtttatag ggctgttaat tttatattat
    10861 atctgccgtt tgatttcaga agaacgtcta tatcgtttta ttgatcgaca cggtaagtgg
    10921 attaaattga aaagtaagga tttaaggcga gcaaatgatt ggtttaaaaa gtatggcgta
    10981 tgggctgtat ttatctgtcg tttcatacct gtattacgtg tattgattac cattccagcc
    11041 ggtgtaaacc gcatgaatgt tgtgacattt accgttattt cattaatagg tacaacaatt
    11101 tggaatttcg gtttaatttt actaggacgc acgttgagcg atagctttgg tatgttgatg
    11161 actggtcttc atacatattc acgtattatg tatgttgtca tcattatcgc agtcatttac
    11221 tttgctatac gttacattgg caaacgtaag agagtaaaat aatattgttg ttagtataaa
    11281 ggcatgaatt ttaaatttct atacacaaaa agccgtaaaa tcattccatc tattgaaatg
    11341 attcactgct tttttaagtt tatttggcat actttagctg aaagtcacct aaaatccttt
    11401 acgttaagag atataatgaa gtttcccatc aaagttcagt tccctcagta tgtatacatt
    11461 ccgcaaactt atctcacaaa tccatctata atacaaacta tcatcttaaa taaacagaat
    11521 ttaccctgtc ctccacacaa attgttttcg acactttaat gtgcctacta tccaaaaaag
    11581 ttactcataa caaattgcgc attttaataa atgtagcaat accatttgca cgctacaaaa
    11641 a
//
LOCUS       DANBWH010000076        11633 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.079, whole genome shotgun sequence.
ACCESSION   DANBWH010000076 DANBWH010000000
VERSION     DANBWH010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11633
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.079"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            109..6669
                     /gene="sasC"
                     /locus_tag="PPY92_001390"
     CDS             109..6669
                     /gene="sasC"
                     /locus_tag="PPY92_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored repetitive surface protein SasC"
                     /protein_id="HDJ2625401.1"
                     /translation="MNLLKKNKYSIRKYKVGIFSTLIGTVLLLSNPNGAQALTTDNNV
                     QSDTNQATPVNSQDTNVANNRGLANSAQNTPNQSATTNQSTNQALVNHNNGSIANQAT
                     PTSVQSSTPSAQNNNHTDGNTTATETVSNANNKDVVSNNTTLNVPNKTNENGSGGHLT
                     LKEIQEDVRHSSDKPELVAIAEQASNRPKKRSRRAAPADPNATPADPAAAAAGNGGAP
                     VAITAPYTPTTDPNANNAGQNAPNEVLSFDDNGIRPSTNRSVPSVTVVDNLPGFTLIN
                     GGKVGVFSHAMVRTSMFDSGDAKNYQAQGNVIALGRIKGNDTNDHGDFNGIEKSLTVN
                     PNSELIFEFNTMTTKNYQGVTNLIIKNADNDTVIAEKSVAYGPIWRLFKVPENVSHLK
                     IQFVPKNDAITDARGIYQLRDGYKYYDFVDSIGLHSGSHVYVERRTMEPTATNNKEFT
                     VTTSLKNNGNFGASFNTDDFVYKVQLPEGVEYVNNSLTKDFPSSNSGVDMNDFNVTYD
                     AANRVITIKSTGGGSGNSPARLMPDKILDLKYKLRVNNVPTPRTVTFNDTLTYKTYTQ
                     DFINSPAESHTVSTNPYTIDIIMNKDALQAEVDRRIQQADYTFASLDIFNDLKRRAQT
                     ILDENRNNVPLNKRVSQADIDSLTNQMQHTLIRSVDAENAVNKKVDQMEDLVNQNDEL
                     TDEEKQAAIQVIEEHKNEIIGNIGDQTTDDGVTRIKDQGIQTLSGDTATPVVKPNAKK
                     AIRDKATKQREIINATPDATEDEIQDALNQLATDETDAIDNVTNATTNADVEIAKNNG
                     INTIGAVVPQVTHKQAARDAINQATATKRQQINSNREATQEEKNAALNELTQATNHAL
                     EQINQATTNADVDNAKGDGLNAINPIAPVTVVKQAARDAVSHDAQQHIAEINANPDAT
                     QEERQAAIDKVNAAVTAANTNILNANTNADVEQVKTNAIQGIQAITPATKVKTDAKNA
                     IDKSAETQHNTIFNNNDATLEEQQAAQQLLDQAVATAKQNINAADTNQEVAQAKDQGT
                     QNIVVIQPATQVKTDARNAVNDKAREAITNINATPGATREEKQEAINRVNTLKNRALT
                     DIGVTSTTAMVNSIRDDAVNQIGAVQPHVTKKQTATGVLNDLATAKKQEINQNTNATT
                     EEKQVALNQVDQELATAINNINQADTNAEVDQAQQLGTKAINAIQPNIVKKPAALAQI
                     NQHYNAKLAEINATPDATNDEKNAAINTLNQDRQQAIESIKQANTNAEVDQAATVAEN
                     NIDAVQVDVVKKQAARDKITAEVAKRIEAVKQTPNATDEEKQAAVNQINQLKDQAINQ
                     INQNQTNDQVDTTTNQAVNAIDNVEAEVVIKPKAIADIEKAVKEKQQQIDNSLDSTDN
                     EKEVASQALAKEKEKALAAIDQAQTNSQVNQAATNGVSAIKIIQPETKVKPAAREKIN
                     QKANELRAKINQDKEATAEERQVALDKINEFVNQAMTDITNNRTNQQVDDTTSQALDS
                     IALVAPEHIVRAAARDAVKQQYEAKKQEIEQAEHATDEEKQVALNQLANNEKLALQNI
                     NQAVTNNDVKRVETNGIATLKGVQPHIVIKPEAQQAIKATAENQVESIKDTPHATVDE
                     LDEANQLISDTLKQAQQEIENTNQDAAVTDVRNQTIKAIEQIKPKVRRKRAALDSIEE
                     NNKNQLDAIRNTLDTTQDERDVAIDTLNKIVNTIKNDIAQNKTNAEVDRTETDGNDNI
                     KVILPKVQVKPAARQSVGVKAEAQNALIDQSDLSTEEERLAAKHLVEQALNQAIDQIN
                     HADKTAQVNQDSIDAQNIISKIKPATTVKATALQQIQNIATNKINLIKANNEATDEEQ
                     NIAIAQVEKELIKAKQQIASAVTNADVAYLLHDEKNEIREIEPVINRKASAREQLTTL
                     FNDKKQAIEANIQATVEERNSILAQLQNIYDTAIGQIDQDRSNAQVDKTASLNLQTIH
                     DLDVHPIKKPDAEKTINDDLARVTALVQNYRKVSNRNKADALKAITALKLQMDEELKT
                     ARTNADVDAVLKRFNVALSDIEAVITEKENSLLRIDNIAQQTYAKFKAIATPEQLAKV
                     KVLIDQYVADGNRMIDEDATLNDIKQHTQFIVDEILAIKLPAEATKVSPKEIQPAPKV
                     CTPIKKEETHESRKVEKELPNTGSEGMDLPLKEFALITGAALLARRRTKNEKES"
     gene            complement(6786..8054)
                     /locus_tag="PPY92_001391"
     CDS             complement(6786..8054)
                     /locus_tag="PPY92_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDJ2625402.1"
                     /translation="MYQTIIIGGGPSGLMAAVAASEQSSSVLLIEKKKGLGRKLKISG
                     GGRCNVTNRLPYAEIIKNIPGNGKFLYSPFSIFDNESIIDFFESRGVKLKEEDHGRMF
                     PVSNKAQDVVDTLVTTIERQHVTIKEEEAVSRIEVNTDQTFTVHTQNNSYESHSLVIA
                     TGGTSVPQTGSTGDGYKFAQDLGHTITELFPTEVPITSAEPFIKSNRLKGLSLKDVEL
                     SVLKKNGKKRISHQMDMLFTHFGISGPAALRCSQFVYKEQKNQKTQHISMAIDAFPEL
                     NHEQLKQHITSLLSDTPDKIIKNSLHGLIEERYLLFMLEQAGIDENTTSHHLSNQQLN
                     DLVNMFKGFVFKVNGTLPIDKAFVTGGGVSLKEIQPKTMMSKLVPGLFLCGEVLDIHG
                     YTGGYNITSALVTGHVAGLYAGHYSHTSME"
     gene            8461..10122
                     /locus_tag="PPY92_001392"
     CDS             8461..10122
                     /locus_tag="PPY92_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDJ2625403.1"
                     /translation="MSESKEMVRGTFLITISILITKVLGVLFIIPFNYLIGGQENMAP
                     FTYAYAPYNIAIAVATAGVPLAASKYVAKYNAIGAYKVSQKFYKSSFIVMSITGVLGF
                     LVLYFLAPYISELTLARNIHDKNGWSVDDITWIIRIISMVVIFIPVLATWRGIFQGYK
                     SMGPTAVSEVTEQIARVIFILIGSYLVLNVFDGSILLANGIATFAAAVGAIIGIFTLW
                     YYWRKRKHNIDRMVESDYTDIDVSYGKMYKEIIAYSIPFVIVSLNYPLFNLVDQFTHN
                     GALSLVGIPSQLQDIFFNMLNMSTNKIVMIPTSLSAGFAVSLIPYITKTFAEGRLHEM
                     HHQIRTSIGVLMFITVPASIGIMALAQPLFTVFYGYDPIVLGHDPNHDGSRLLFYYAP
                     VAILISLLSVTASMLQGIDKQKLTVYVILASVVIKLALNYPLIMLFHTPGAILSTSIA
                     LLFAIGCNFYILKKYAKFKFSYSWIHFAKIFLYSFIMMLGVELVFFLANLFLEPTKLG
                     YLIIIILGVTVGILIYGTITIKTRLADEFLGEIPEKLRRRVRFLR"
     gene            10119..10814
                     /locus_tag="PPY92_001393"
     CDS             10119..10814
                     /locus_tag="PPY92_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDJ2625404.1"
                     /translation="MRIDKFLANMGVGTRNEVKQLLKKGLVNVNEQVIKSPKTHIEPE
                     NDKITVRGELIEYIENVYIMLNKPKGYISATEDHHSKTVIDLIPEYQHLNIFPVGRLD
                     KDTEGLLLITNDGDFNHELMSPNKHVSKKYEVISANPITEDDIQAFKEGVTLTDGKVK
                     PAILTYIDNQTSHVTIYEGKYHQVKRMFHSIQNEVLHLRRIKIADLELDSNLDSGEYR
                     LLTENDFDKLNYK"
     gene            10831..11253
                     /locus_tag="PPY92_001394"
     CDS             10831..11253
                     /locus_tag="PPY92_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDJ2625405.1"
                     /translation="MAKGNLFKAILGIGGAVAAVLVTRKDSRDKLKAEYNKYKQDPQS
                     YKDNAKDKATQLGTIANETIKEVKTNPKEYANRLKNNPKAFFEEEKSKFTEYDNKTDE
                     SIEKGKFDDEGGAAPNNNLRIVTEEDLKKNKNALSDKE"
BASE COUNT         4438 a         1854 c         2060 g         3281 t
ORIGIN      
        1 aaatattgta caaaataata tagctataaa cataatatga gagagcgtat atttttagca
       61 taattctaaa caacacagca gagaacagac aaccaggagg aaaatgaaat gaatttgtta
      121 aagaaaaata aatatagtat taggaagtat aaagtaggca tattctctac tttaatcgga
      181 acagttttat tactttcaaa cccaaatggt gcacaagcct taactacgga taataatgta
      241 caaagcgata ctaatcaagc cacacctgta aattcacaag atacaaatgt tgctaataat
      301 agaggtttag caaatagtgc tcagaataca cctaatcaat ctgcaacaac taatcaatca
      361 acgaatcaag cattggttaa tcataataat ggtagtatag caaatcaagc tacgccaaca
      421 tcagtgcaat caagtacgcc ttcagcacaa aacaataatc atacagatgg caatacaaca
      481 gcaactgaga cagtgtcaaa cgctaataat aaggatgtag tgtcgaataa taccacatta
      541 aatgtaccta ataaaacaaa tgaaaatggt tcaggaggac atctaacttt aaaggaaatt
      601 caagaagatg ttcgtcattc ttcagataaa ccagagctag ttgcaattgc tgaacaagca
      661 tctaatagac cgaaaaagag aagtagacgt gcggcaccgg cagatcctaa tgcaactcca
      721 gcagatccag cggctgcagc ggcaggaaac ggtggtgcac cagtagcaat tacagcgcca
      781 tacacgccga caactgatcc taatgccaat aatgcaggac aaaatgcacc taacgaagtg
      841 ctgtcttttg atgacaatgg tattagacca agtaccaacc gttctgtacc atcagtaaca
      901 gttgttgata atttaccagg ctttacactt atcaatggtg gtaaagtagg tgtgtttagt
      961 catgcgatgg ttagaacaag tatgtttgat tcaggagatg ctaagaacta tcaagcacaa
     1021 ggaaatgtaa ttgcattagg tcgtattaag ggaaatgata caaatgatca tggtgatttt
     1081 aatggaattg aaaaatcttt aacagtaaat ccaaattctg aattaatttt cgaatttaat
     1141 acgatgacta caaaaaacta tcaaggtgtg acaaatttaa taattaaaaa tgctgataat
     1201 gatactgtta ttgcagaaaa atcagtagct tatggtccga tttggcgttt gtttaaagtg
     1261 cctgaaaatg ttagtcactt aaaaattcaa tttgtaccta aaaatgatgc aataacagat
     1321 gcgcgtggca tttatcaact aagagacggt tataaatact atgactttgt tgactcaatt
     1381 ggtctacatt ctggttcgca tgtctatgtt gaaagacgaa caatggaacc cacagctact
     1441 aataataaag aatttacagt cacaacttca ttaaagaata atggtaactt cggtgcttca
     1501 tttaatacag atgattttgt atataaagtt caattacctg aaggggtaga atatgttaat
     1561 aactcattga ctaaagattt tccaagcagt aattcaggtg ttgatatgaa tgattttaat
     1621 gtgacgtatg atgcggcgaa tcgagtgata acaattaaaa gtactggtgg aggttcaggt
     1681 aactcgccgg cacgattaat gcctgataaa atattggatt taaagtataa gttgcgtgtt
     1741 aataatgtac cgacaccaag aacagtaaca tttaatgata cattaacata taaaacatat
     1801 acacaagatt ttattaattc acctgctgaa agtcatactg taagtacaaa tccatataca
     1861 atcgatatca tcatgaataa agatgcatta caagccgagg ttgatagacg cattcaacaa
     1921 gctgattata catttgcatc attagatatc tttaatgatc ttaaaagacg tgcacaaacg
     1981 attttagatg aaaatcgtaa caatgtacca ttaaataaaa gagtttctca agcagatatt
     2041 gattcattaa ctaatcaaat gcaacatacg ttaattcgaa gtgttgatgc tgaaaatgca
     2101 gttaataaaa aagttgacca aatggaagat ttagttaatc aaaatgatga attgacagat
     2161 gaagaaaaac aagcagcaat acaagttatc gaggaacata aaaatgaaat aattggtaat
     2221 attggtgacc aaacgactga tgatggcgtt actagaatca aagatcaagg tatacagacc
     2281 ttaagtgggg atactgcaac accggttgtt aaaccaaatg ctaaaaaagc aatacgtgat
     2341 aaagcaacga aacaaaggga aattatcaat gcaacaccag atgctactga agacgagatt
     2401 caagatgcac taaatcaatt agctacggat gaaacagatg ctattgataa tgttacgaat
     2461 gctactacaa atgctgacgt tgaaatagct aaaaataatg gcatcaatac tattggagca
     2521 gttgttcctc aagtgacaca taaacaagct gcaagagatg caattaacca agcaacagca
     2581 acgaaaagac aacaaataaa tagtaataga gaagcaactc aggaagagaa aaatgcagca
     2641 ttgaacgaat taactcaagc aaccaaccat gctttagaac aaatcaatca agcaacaaca
     2701 aatgctgatg ttgataacgc caaaggagat ggtctaaatg ccattaatcc aattgctcct
     2761 gtaactgttg ttaagcaagc tgcaagggat gctgtatcac atgatgcaca acaacatatc
     2821 gcagaaatca atgctaatcc tgatgcgact caagaagaaa gacaagcagc aattgacaaa
     2881 gtgaatgctg ctgtaactgc agcaaacaca aacattttaa acgctaatac caatgctgat
     2941 gttgaacaag taaagacaaa tgcgattcaa ggaatacaag caattacacc agctacaaaa
     3001 gtaaaaacag atgcaaaaaa tgccatcgat aaaagtgcgg aaacgcaaca taatacgata
     3061 tttaataata atgatgcgac gctcgaagaa caacaagcag cacaacaatt acttgatcaa
     3121 gctgtagcca cagcgaagca aaatattaat gcagcagata cgaatcaaga agttgcacaa
     3181 gcaaaagatc agggcacaca aaatatagtt gtgattcaac cggcaacaca agttaaaacg
     3241 gatgctcgca atgctgtaaa tgataaagct cgagaggcga taacaaatat caatgctaca
     3301 cctggcgcga ctcgagaaga gaaacaagaa gcgataaatc gtgtcaatac acttaaaaat
     3361 agagcattaa ctgatattgg tgtgacgtct actactgcga tggtcaatag tattagagac
     3421 gatgcagtca atcaaatcgg cgcagttcaa ccgcatgtaa cgaagaaaca aactgctaca
     3481 ggtgtattaa atgatttagc aactgctaaa aagcaagaaa ttaatcaaaa cacaaatgca
     3541 acaactgaag aaaagcaagt ggctttaaat caagtggatc aagagttagc aacggcaatt
     3601 aataatataa atcaagctga tacaaatgcg gaagtagatc aagcgcaaca attaggtaca
     3661 aaagcaatta atgcgattca gccaaatatt gttaaaaaac ctgcagcatt agcacaaatc
     3721 aatcagcatt ataatgctaa attagctgaa atcaatgcta caccagatgc aacgaatgat
     3781 gagaaaaatg ctgcgatcaa tactttaaat caagacagac aacaagctat tgaaagtatt
     3841 aaacaagcta acacaaatgc agaagtagac caagctgcga cagtagcaga gaataatatc
     3901 gatgctgttc aagttgatgt agtaaaaaaa caagcagcgc gagataaaat cactgctgaa
     3961 gtggcgaagc gtattgaagc ggttaaacaa acacctaatg caactgacga agaaaagcag
     4021 gctgctgtta atcaaatcaa tcaacttaaa gatcaagcaa ttaatcaaat taatcaaaac
     4081 caaacaaatg atcaggtaga cacaactaca aatcaagcgg taaatgctat agataatgtt
     4141 gaagctgaag tagtaattaa accaaaggca attgcagata ttgaaaaagc tgttaaagaa
     4201 aagcaacagc aaattgataa tagtcttgat tcaacagata atgagaaaga agttgcttca
     4261 caagcattag ctaaagaaaa agaaaaagca cttgcagcta ttgaccaagc tcaaacgaat
     4321 agtcaggtga atcaagcagc aacaaatggt gtatcagcga ttaaaattat tcaacctgaa
     4381 acaaaagtta aaccagctgc acgtgaaaaa atcaatcaaa aagcgaatga attacgtgct
     4441 aagattaatc aggataaaga agcaacagca gaagaaagac aagtagcact agataaaatc
     4501 aatgaatttg taaatcaagc catgacagat attacgaata atagaacaaa tcaacaagtt
     4561 gatgatacaa caagtcaagc gcttgatagc attgctttag tagcgcctga acatattgtt
     4621 agagcagctg ctagagatgc agtaaagcaa caatatgagg ctaaaaagca agaaatagag
     4681 caagctgaac atgcgactga tgaagaaaaa caagttgctt taaatcaatt agcgaataat
     4741 gaaaaacttg cattacaaaa catcaatcaa gcagtaacga ataatgatgt gaaacgtgtt
     4801 gaaacaaatg gcattgctac actaaaaggt gtacaacctc atattgtaat taagcctgaa
     4861 gcacaacaag caataaaagc aactgcagaa aatcaagtag aatcaataaa agatacacca
     4921 catgcaacag ttgatgaatt agatgaagcg aatcaattaa ttagcgacac actcaaacaa
     4981 gcgcaacaag aaatagaaaa tacaaatcaa gatgctgctg ttactgatgt tagaaatcaa
     5041 acaatcaagg caattgagca aataaaacct aaagtaagac gtaaacgagc tgcgcttgat
     5101 agcattgaag aaaataataa aaatcaactc gatgcaatcc gaaatacgtt ggatactact
     5161 caagatgaaa gagatgttgc tattgatact ttaaataaaa ttgtaaatac aattaaaaat
     5221 gacattgcac aaaacaaaac gaatgcagaa gtggatcgaa ctgagactga tggcaacgac
     5281 aacatcaaag tgattttacc taaagttcaa gttaaaccag cagcgcgtca atctgttggt
     5341 gtaaaagccg aagctcaaaa tgcactaatc gatcaaagcg atttatcaac tgaagaagaa
     5401 agactagctg ctaaacattt agtagaacaa gcacttaatc aggctattga tcagatcaat
     5461 catgcagata agactgccca agttaatcaa gatagtatag atgctcaaaa tattatttca
     5521 aaaattaaac cagcgacaac agttaaagca acagcattac aacaaattca aaatatcgct
     5581 acaaataaaa ttaatttaat taaagcaaat aacgaagcga cagatgaaga acaaaatatt
     5641 gcaatagcac aagttgaaaa agagttaatt aaagctaaac aacaaattgc tagtgcagtg
     5701 actaatgctg atgtggcata tttattgcat gatgagaaaa acgaaattcg tgaaatcgaa
     5761 cctgttatta acagaaaggc gtctgctcga gaacaattga caacattatt caacgataaa
     5821 aaacaagcaa ttgaagcgaa tattcaagca acggtagaag aaagaaatag tatattagca
     5881 cagttacaaa atatttatga cactgctatt ggacaaattg atcaagatcg tagcaatgca
     5941 caagttgata aaacagcatc attaaatcta caaacaatac atgatttaga tgtacatcct
     6001 attaaaaagc cagatgctga aaaaacgatt aatgatgatc ttgcacgcgt cactgcttta
     6061 gtgcaaaatt atcgaaaagt aagtaatcgt aataaggctg atgcattaaa agctataact
     6121 gctttaaaat tacaaatgga tgaagaatta aaaacagcac gcactaatgc tgatgttgat
     6181 gcagttttaa aacgatttaa tgttgcatta agcgatatag aagcagtaat tactgaaaaa
     6241 gaaaatagct tactgcgaat tgataacatt gctcaacaaa catatgcgaa attcaaagcg
     6301 atcgcaacac cagaacaatt agctaaagta aaagtattaa ttgatcaata tgttgcagat
     6361 ggcaatagaa tgattgatga agatgcgaca ttaaatgaca tcaaacaaca cacgcaattc
     6421 attgttgatg aaattttagc aattaaatta ccagctgaag cgacgaaagt atcaccaaaa
     6481 gaaattcagc cagctccaaa agtttgtacg cctattaaaa aagaagagac acatgaatcg
     6541 cgcaaagttg aaaaagaact tccaaataca ggttctgaag gaatggattt accattgaaa
     6601 gaatttgcac tgattacagg tgcggctttg ttagctagaa gacgtactaa aaacgaaaaa
     6661 gaatcataat taacgaaatg accttaatat aacgacactt tttagtgact cagttatatt
     6721 aaggtcattt ttaattgaat gaaaaagttg atctactaaa gagaatcgaa ccaaatttta
     6781 tactattatt ccattgatgt atgtgagtaa tgaccggcat ataatccagc gacatgtcct
     6841 gttacgagtg cacttgtaat attataacca ccagtataac catgtatatc taatacttca
     6901 ccacataaaa ataatcccgg aactaattta gacatcattg ttttaggttg aatttcttta
     6961 agtgacacac caccacctgt gacaaatgcc ttatctatag gtaatgtccc gtttacctta
     7021 aatacaaacc ctttaaacat atttactaag tcgttcaatt gttgatttga taagtgatgt
     7081 gatgtggtat tttcatcgat tccagcttgt tccagcatga acagtaagta gcgctcttca
     7141 attagaccat gcaaactgtt tttaatgatt ttatctggtg tgtccgataa taatgatgtg
     7201 atgtgttgtt ttaattgttc atggtttaat tcaggaaatg catcgattgc catagaaatg
     7261 tgctgtgtct tttgattttt ttgttcttta taaacaaact gactacatct taatgcagct
     7321 ggaccactga taccaaaatg agtaaataac atatccattt gatgactgat gcgtttttta
     7381 ccatttttct taagtactga caattcaaca tcttttaaac ttaaaccttt tagacgattg
     7441 gatttgatga aaggttcagc tgatgtaatt ggaacttcgg tcgggaataa ctcagtaatg
     7501 gtatgaccta aatcttgtgc gaacttataa ccatcaccag ttgaaccagt ttgagggaca
     7561 cttgtaccac ctgtagcaat cactagcgaa tggctttcat aactattatt ttgagtatgt
     7621 acagtgaaag tttggtctgt attaacttcg attctactaa cagcttcttc ttctttaatc
     7681 gttacatgtt ggcgttcgat agttgtcact aatgtatcaa ccacgtcttg tgctttgttg
     7741 gaaactggaa acatacgccc gtgatcttct tcttttaatt taacacccct agactcaaaa
     7801 aaatctatga tggattcatt atcaaaaatt gaaaagggac tatataaaaa tttcccattt
     7861 ccaggaatgt tcttaataat ttcagcatat ggtaatcgat tagttacgtt acatctaccg
     7921 ccaccagata ttttgagttt acgacctaga cctttctttt tttcaatgag taacacactg
     7981 ctactttgtt cgcttgcagc tactgccgcc attaagccgc taggtccgcc tccgataata
     8041 attgtttgat acatatacgc ttcctccaaa tttgttactg gacattcaat tgctatttta
     8101 tagtactttt ttaaactttg ctatatctct tgtgttatga gatgacaatt atgttgaata
     8161 tatttataat gtgaacttca taaaaaatga agatgtatgt gtatgataag aatgattttt
     8221 ctactgttat gcgatgttca tctcaaatag tagtataatg tttatatgaa atttcgtaat
     8281 attctaatca aaatcgagta ttcaatgaca atgcgttgat aattttgtta tgataattca
     8341 ttgaatatac gacaatgaaa taaagttttg tgatatattc ataataatga aaaatgattt
     8401 tacagagaaa tagcttttag tttacaaaac tttaataaac atgagatagg gagattcgta
     8461 atgagtgaaa gtaaagaaat ggtgcgtgga acctttttaa ttaccattag tatattaatt
     8521 accaaggtat tgggcgtact ttttatcatt ccattcaact atttaattgg tggacaagaa
     8581 aatatggcgc cgttcacata cgcttatgca ccatataata ttgcaattgc agttgcgaca
     8641 gcaggtgtac cattagcagc ttcgaaatat gttgcgaaat ataatgctat tggggcatat
     8701 aaagtaagtc agaaatttta taaatcgagt tttattgtaa tgagtattac cggtgtttta
     8761 ggtttcttgg tactgtattt cttagcacct tatatttctg aattaacatt agctagaaac
     8821 attcatgata aaaacggttg gtcagtcgat gatattactt ggattataag aatcattagt
     8881 atggttgtta tctttattcc tgtattagcg acatggagag ggatattcca aggttataaa
     8941 tctatggggc caacagcagt gtcagaagta actgagcaaa ttgcacgcgt gattttcata
     9001 ttaatcggaa gttatttagt actaaatgtc tttgatggct ctattttatt agccaatggt
     9061 attgctactt ttgcagcagc agttggtgcg attattggta tattcacact ctggtattac
     9121 tggagaaagc gtaagcataa tattgatcgt atggtcgaat ctgattacac agatatagat
     9181 gtgtcatatg gcaagatgta taaagaaatt attgcttata gtataccgtt cgttattgta
     9241 agtttgaatt atccattatt taacttagta gatcaattta cacataacgg tgctttatct
     9301 ttagttggta taccttcaca gttacaagat atattcttta atatgctaaa tatgtcaact
     9361 aataaaattg taatgattcc gacatcatta agtgctggtt ttgcagtgag tttaattcct
     9421 tatattacaa aaacatttgc agaaggtcgc ttacatgaaa tgcatcatca aattagaaca
     9481 tctattggtg tattaatgtt tataactgtt ccagctagta ttggaattat ggccttggca
     9541 caaccattat tcacagtttt ctatggttac gatccaattg ttttaggtca tgaccctaac
     9601 catgatggta gtcgcttatt attctattat gcgcctgtag caatactaat ttcactatta
     9661 agtgtaacgg catcaatgtt acaaggtata gataaacaga aattaacagt ttatgttatt
     9721 ttagcttctg tagttatcaa gttagcgtta aactatccat taattatgtt attccataca
     9781 cctggtgcaa ttttaagtac tagtatcgca ttattatttg caattggatg taatttctat
     9841 attcttaaaa aatatgcgaa gtttaaattc agctatagtt ggattcattt tgcaaaaata
     9901 ttcttgtatt catttattat gatgctaggt gtagaattag tatttttcct tgcaaatcta
     9961 ttcttagaac ctactaaact tggttactta atcattatta ttttaggtgt tacagtaggt
    10021 attctaattt acggtacaat cacaattaaa acgcgtcttg cggacgaatt cttaggtgaa
    10081 attcctgaaa aattaagacg tagagttagg tttttacgat gagaatagat aaatttttag
    10141 caaatatggg cgtcggtaca cgcaatgagg tcaaacagtt acttaaaaaa ggccttgtaa
    10201 atgtcaacga acaagtaatt aaatcaccaa aaacacatat agaaccagaa aacgataaga
    10261 taacagttcg tggagaatta atagaataca ttgaaaatgt ctatattatg ttaaataagc
    10321 caaaaggtta tatatcagca actgaagatc atcattcaaa aaccgttatt gatttaatac
    10381 ctgaatacca acatctaaat atttttccgg ttggtcgtct tgataaagat acagaagggc
    10441 ttttattgat aacgaatgat ggtgatttta atcatgaatt aatgagtcca aacaaacatg
    10501 tttctaaaaa atatgaagtt atttcagcaa atcctatcac tgaagacgac attcaagctt
    10561 ttaaagaggg tgtaacactg actgatggca aggttaagcc tgcaatatta acttatattg
    10621 ataatcaaac gtcacacgta acaatttatg aaggaaaata tcatcaagtt aaacgaatgt
    10681 ttcatagcat tcaaaatgag gtattacatt taagacgtat caaaattgct gatttagaat
    10741 tggacagcaa tttagattca ggagaatatc gtttattaac tgaaaatgat tttgacaaat
    10801 taaattataa ataaaaggga gacgattgat atggcaaaag gtaatttatt taaagcgatt
    10861 ttaggtatag gtggcgctgt agcagctgta cttgttacac gtaaagatag tcgtgacaag
    10921 ctgaaagcag aatataataa atataaacaa gatcctcaaa gctataaaga taatgctaag
    10981 gataaagcga cgcaattagg aacaattgct aatgaaacaa ttaaagaagt aaaaacaaat
    11041 ccgaaagaat atgctaatag attaaaaaat aatccaaaag catttttcga agaagaaaaa
    11101 tcaaaattta ccgaatatga caataagact gacgaaagta ttgaaaaagg taaatttgat
    11161 gatgaaggtg gcgcagcacc aaataataat ttacgtatcg tcactgaaga agatttaaaa
    11221 aagaataaaa atgcattatc tgataaagaa taagatgtga gtaggacaga aatgataaag
    11281 aaccactatg atttattata tagtggttct ttatcgttag tcatagctaa catgcatata
    11341 gaaagaagaa tacatgcgtg aaactcattc ataagaaata ctaatatcta aagaaaacgt
    11401 atttctttat cttttcgaaa ttctctgtgt tggggccctt accccaacct gcacattatt
    11461 gaaagctgac tattggccag cttctgtgtt ggggccccgc caacttgcat tgtctgtaga
    11521 atttcttttc gaaattctct gtgttggggc cccggggcgc attttcgttc ggttaattac
    11581 tgtcaatatt aaattgtgtg cctagtacat tgacttatat ctcgagctct tga
//
LOCUS       DANBWH010000077        11275 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.080, whole genome shotgun sequence.
ACCESSION   DANBWH010000077 DANBWH010000000
VERSION     DANBWH010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11275
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.080"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(207..1085)
                     /locus_tag="PPY92_001395"
     CDS             complement(207..1085)
                     /locus_tag="PPY92_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HDJ2625406.1"
                     /translation="MTEFDLSTREGRWKHFGSVDPIEGTKPTTKNEMTDLQSTHKDFL
                     FEIEEVGIKNLVYPVLVDQYQTAGTFSFSTSLTKDEKGINMSRIIESVEKHYDNGIEL
                     EFNTLYQVLRTLQTNMKQNAAGVDVSGKWFFDRYSPTTNIKAVGNADVTYGLAIDGDK
                     VTRKELTIEATVTTLCPCSKEISEYSAHNQRGVVTVKTYINKDQNIVDDYKNKILDAM
                     EANASSILYPILKRPDEKRVTERAYENPRFVEDLIRLIAADLVEFDWLDGFDIECRNE
                     ESIHQHDAFAKLKYRK"
     gene            complement(1098..1763)
                     /gene="bshB2"
                     /locus_tag="PPY92_001396"
     CDS             complement(1098..1763)
                     /gene="bshB2"
                     /locus_tag="PPY92_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB2"
                     /protein_id="HDJ2625407.1"
                     /translation="MTDERHVLVIFPHPDDETFSSAGTLASYIQKGIPVTYACLTLGQ
                     MGRNLGNPPFATRESLPSIRERELEEACKVIGITDLRKMGLRDKTVEFEPYEHIDGMI
                     KSLIDDTNPSLIISFYPGYAVHPDHEATADAVIRTVERMPKEERPRLTLVAFSNDATE
                     ALGEPDIQNDITDFKELKIKAFEAHASQTGPFLKQLASPEIDGKQHSFLTVEPYWTYH
                     FKS"
     gene            complement(1777..2139)
                     /locus_tag="PPY92_001397"
     CDS             complement(1777..2139)
                     /locus_tag="PPY92_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YojF family protein"
                     /protein_id="HDJ2625408.1"
                     /translation="MLEPIKEQEVIDLLASFEHKPVYLHVETTNGAYANHFDQRVFNA
                     GTFLRNIQVTYTHAQLKGGNKEPYRIGLKLSNGGWVYVQGLTHFEVNEHDEFLIAGFN
                     YEGQLAAALQISERPFNL"
     gene            2415..3173
                     /gene="nagB"
                     /locus_tag="PPY92_001398"
     CDS             2415..3173
                     /gene="nagB"
                     /locus_tag="PPY92_001398"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDJ2625409.1"
                     /translation="MKVLNLGSKKQASFYVACELYKEMAFNQHCKLGLATGGTMTDLY
                     EQLVKLLNKNQLNVDNVSTFNLDEYVGLTASHPQSYHYYMDDMLFKQYPYFNRKNIHI
                     PNGDADDMNAEASKYNDVLEQQGQRDIQILGIGENGHIGFNEPGTPFDSVTHIVDLTE
                     STIKANSRYFKNEDDVPKQAISMGLANILQAKRIILLAFGEKKRAAITHLLNQEISVD
                     VPATLLHKHPNVEIYLDDEACPKNVAKIHVDEMD"
     gene            3250..3882
                     /gene="hxlA"
                     /locus_tag="PPY92_001399"
     CDS             3250..3882
                     /gene="hxlA"
                     /locus_tag="PPY92_001399"
                     /EC_number="4.1.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hexulose-6-phosphate synthase"
                     /protein_id="HDJ2625410.1"
                     /translation="MELQLAIDLLNKEDAAELANKVKDYVDIVEIGTPIIYNEGLPAV
                     KHMADNISNVKVLADMKIMDAADYEVSQAIKFGADVITILGVAEDASIKAAIEEAHKN
                     NKQLLVDMIAVQDLEKRAKELDEMGADYIAVHTGYDLQAEGQSPLESLRTVKSVIKNS
                     KVAVAGGIKPDTIKDIVAESPDLVIVGGGIANADDPVEAAKQCRAAIEGK"
     gene            3884..4432
                     /gene="hxlB"
                     /locus_tag="PPY92_001400"
     CDS             3884..4432
                     /gene="hxlB"
                     /locus_tag="PPY92_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HDJ2625411.1"
                     /translation="MAKFSDYQLILDELKMTLSHVEADEFSTFASKILHAEHIFVAGK
                     GRSGFVANSFAMRLNQLGKQAHVVGESTTPAIKSNDVFVIISGSGSTEHLRLLADKAK
                     SVGADIVLITTNKDSAIGNLAGTNIVLPAGTKYDEQGSAQPLGSLFEQASQLFLDSVV
                     MGLMTEMNVTEQTMQQNHANLE"
     gene            4545..5192
                     /locus_tag="PPY92_001401"
     CDS             4545..5192
                     /locus_tag="PPY92_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDJ2625412.1"
                     /translation="MKFDNYIFDFDGTLADTKKCGEVATQSAFKACGLTEPSSKEITH
                     YMGIPIEESFLKLADRPLDEAALAKLIDTFRHTYQSIEKDYIYEFAGITEAITSLYNQ
                     GKKLFVVSSKKSDVLERNLSAIGLNHLITEAVGSDQVSAYKPNPEGIHTIVQRYNLNS
                     QQTVYIGDSTFDVEMAQHAGVPSAAVTWGAHDARSLLHSNPDFIINDPSEINTVL"
     gene            5694..7094
                     /locus_tag="PPY92_001402"
     CDS             5694..7094
                     /locus_tag="PPY92_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDJ2625413.1"
                     /translation="MDFNKENINMVDAKKAKKTVVATGIGNAMEWFDFGVYAYTTAYI
                     GANFFSPVENADIRQMLTFAALAIAFLLRPIGGVVFGIIGDKYGRKVVLTSTIILMAF
                     STLTIGLLPSYDQIGLWAPILLLLARVLQGFSTGGEYAGAMTYVAESSPDKRRNSLGS
                     GLEIGTLSGYIAASIMIAVLTFFLTDEQMASFGWRIPFLLGLFLGLFGLYLRRKLEES
                     PVFENDVATQPERDNINFLQIIRFYYKDIFVCFVAVVFFNVTNYMVTAYLPTYLEQVI
                     KLDATTTSVLITCVMAIMIPLALMFGKLADKIGEKKVFLIGTGGLTLFSIIAFMLLHS
                     QSFVVIVIGIFILGFFLSTYEATMPGSLPTMFYSHIRYRTLSVTFNISVSIFGGTTPL
                     VATWLVTKTGDPLAPAYYLTAISVIGFLVITFLHLSTAGKSLKGSYPNVDNEQDRAYY
                     AEHPKEALWWVKERKN"
     gene            7638..9014
                     /locus_tag="PPY92_001403"
     CDS             7638..9014
                     /locus_tag="PPY92_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HDJ2625414.1"
                     /translation="MNVILEQLKTHTQNKPNDIALHIDDETITYSQLNARITSAVESL
                     QKYSLNPVVAINMKSPVQSIICYLALHRLHKVPMMMEGKWQSTIHRQLIEKYGIKDVI
                     GDTGLMQNIDSPMFIDSTQLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEM
                     LMLKNENAIAAPGPLSHSLTLYALLFALSSGRTFIGQTTFHPEKLLNQCHKISSYKVA
                     MFLVPTMIKSLLLVYNNEHTIQSFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETS
                     FISYNLNQQAPVESVGVLFPNVELKTTNHDHNGIGTICIKSNMMFSGYVSEQCINNDE
                     WFVTNDNGYVKEQYLYLTGRQQDMLIIGGQNIYPAHVERLLTQSSSIDEAIIIGIPNE
                     RFGQIGVLLYSGDVTLTHKNVKQFLKKKVKRYEIPSMIHHVEKMYYTASGKIAREKMM
                     SMYLRGEL"
     gene            9016..10155
                     /locus_tag="PPY92_001404"
     CDS             9016..10155
                     /locus_tag="PPY92_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HDJ2625415.1"
                     /translation="MNQAVIVAAKRTAFGKYGGTLKHLEPEQLLKPLFQHFKEKYPEV
                     ISKIDDVVLGNVVGNGGNIARKALLEAGLKDSIPGVTIDRQCGSGLESVQYACRMIQA
                     GAGKVYIAGGVESTSRAPWKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENVA
                     KMYDVSRELQDEFAYRSHQLTAENVKNGNISQEILPITVKGEIFNTDESLKSHIPKDN
                     FGRFKPVIKGGTVTAANSCMKNDGAVLLLIMEKDMAYELGFEHGLLFKDGVTVGVDSN
                     FPGIGPVPAISNLLKRNQLTIENIEVIEINEAFSAQVVACQQALNISNTQLNIWGGAL
                     ASGHPYGASGAQLVTRLFYMFDKETMIASMGIGGGLGNAALFTRF"
     gene            10130..10495
                     /locus_tag="PPY92_001405"
     CDS             10130..10495
                     /locus_tag="PPY92_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraC"
                     /protein_id="HDJ2625416.1"
                     /translation="MQHYLLDSNQRLNVSFSKDSVAAYYQCFNQPYRKEVPPLMCASL
                     WPKFDLFKKYANSELILTKSAINQTQKIEVDTIYVGHLEDIECRQTRNITRYTMALTL
                     TKNDQHVITVTQTFIKAMK"
     gene            10498..10767
                     /locus_tag="PPY92_001406"
     CDS             10498..10767
                     /locus_tag="PPY92_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625417.1"
                     /translation="MKFNEIWINEYLALVNDDNPIHNEIVPGQLVSQMMLMAMSLETN
                     QCQINYVKPILINENIEFIEQHEHEIIAINDDGEIKIKISLSTKK"
     gene            complement(10968..11135)
                     /locus_tag="PPY92_001407"
     CDS             complement(10968..11135)
                     /locus_tag="PPY92_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraX"
                     /protein_id="HDJ2625418.1"
                     /translation="MIIYRQYHHEGAPVYEIITKTFQHVSIKCDDSFSDTEIFKLLSL
                     LQDDIDHMKVS"
BASE COUNT         3892 a         1677 c         2050 g         3656 t
ORIGIN      
        1 gtttatttat aaaacaacaa attttgatat ttataatgat agtagttatt caatcaacta
       61 cgaccaatat atcattgtag agcttaggat attgatttat gcctcaggca catcaaatga
      121 gaagatttat aaaagagata tacaactcta gaaggtataa taaaaacgcg caactaatgt
      181 tacgcgtttg aattaatcat atgatattat ttgcgatact ttaatttagc gaaagcatca
      241 tgttgatgga tagactcttc attacgacat tcgatatcga aaccgtctaa ccaatcaaat
      301 tcaactaagt ccgcggcaat taaacgaatt aagtcttcga caaaacgtgg attttcatat
      361 gcacgctctg tcacacgttt ttcatcagga cgttttaaaa tagggtatag aattgaactt
      421 gcattagctt ccattgcatc taaaattttg tttttatagt catcaactat gttttgatct
      481 ttattaatat atgttttaac agtgacaaca ccacgttggt tgtgcgctga atactcactt
      541 atttcttttg aacaagggca tagcgttgtg acagttgctt caatagtaag ttctttacgt
      601 gtaactttat caccgtcaat tgctaatcca taagtgacat cggcattacc aactgcttta
      661 atatttgtgg ttggactata gcggtcaaag aaccatttcc cagaaacatc aacgcctgcc
      721 gcattttgtt tcatattcgt ttgtaaagtg cgtaacacct gataaagtgt attaaattca
      781 agttcaatac cattatcata gtgcttttca acactttcaa ttatacggct catattaata
      841 cctttttcgt cttttgttaa acttgttgaa aaactaaatg tgccagctgt ttgatactgg
      901 tcaacaagta cagggtacac taagttttta ataccaactt cttctatttc aaataaaaaa
      961 tctttatgtg tactttgtaa atctgtcatt tcgttcttag tagtaggttt cgtgccttca
     1021 ataggatcta cggaaccaaa gtgtttccaa cgaccttctc gtgtcgataa atcaaattca
     1081 gtcatttttt tcctccgtta agatttaaag tgatatgtcc aatatggttc gactgttaaa
     1141 aagctgtgtt gtttaccatc gatttcagga cttgctaatt gttttaaaaa tggacctgtt
     1201 tgagaagcat gtgcttcaaa tgccttaatt ttaagttctt taaaatctgt aatatcattt
     1261 tgaatatcag gttctccaag agcttcggtt gcatcattac tgaacgcaac taaagttaaa
     1321 cgagggcgtt cttctttagg catgcgttca accgttcgaa ttacagcgtc tgctgttgct
     1381 tcgtgatcag gatgtactgc atatccagga taaaatgaaa taatcaatga tggatttgta
     1441 tcatcgatta aagatttaat cataccatct atatgttcgt agggttcaaa ttcgacagtt
     1501 ttgtcacgta aacccatttt tcttaaatca gtaataccaa taactttaca agcttcttct
     1561 agttcacgct cacgaatact tggtaatgat tcgcgtgttg caaatggggg attacctaaa
     1621 tttctgccca tttgtcctag ggttaaacat gcatatgtta caggtatgcc tttttggata
     1681 taacttgcta atgtgcctgc agatgagaag gtttcatcat caggatgtgg aaatattact
     1741 aatacatgtc tttcgtcagt catgttgatg cctcctctat aaattaaatg gtcgctcact
     1801 aatttgaagt gctgcagcga gttgaccttc gtaattaaaa cctgcaatta aaaattcatc
     1861 atgttcattg acctcaaaat gcgttagacc ttgtacataa acccaaccac catttgatag
     1921 tttaagacca atgcgataag gttctttatt accacctttt agttgtgcat gcgtatatgt
     1981 tacttgtatg tttcttaaaa atgtaccagc attaaaaaca cgttgatcga aatggttcgc
     2041 ataggcccca tttgtcgttt caacatgcag atacacaggt ttatgttcaa aagaagcaag
     2101 taaatctata acttcttgtt ctttaattgg ttccaacacg ttcactcctt acactatcaa
     2161 tgtgtttatc tttctatttt actaaaaact attcgataat tgtatacgat tgctcaatta
     2221 tttataaatt aattttcatg aagggtaatt actcaggatt acgtaatcat acagcattag
     2281 ttttttactt ttaaaaatca aaaatttgtt ggaatttgaa aagtgttaaa cattaaaaat
     2341 gatgctatat taatggtgta tgaatgaatt cataagtttt taaaatgtat taaatttgtg
     2401 gaggcatgta aacaatgaaa gtattaaact taggatcgaa aaaacaagca tcattctatg
     2461 ttgcatgtga gttatataaa gagatggcat ttaatcagca ctgtaaacta ggtttagcaa
     2521 ctggtggtac aatgacagat ttgtatgaac aacttgttaa gttattaaat aaaaatcagt
     2581 taaacgtaga caatgtatcc acgtttaatt tagacgaata tgtaggttta accgcatcac
     2641 atccgcaaag ttatcactat tatatggatg acatgctttt caaacaatat ccttatttta
     2701 atagaaagaa cattcatatt ccaaatggag atgccgatga tatgaatgcg gaagcgtcaa
     2761 aatataatga cgttttagaa caacaaggtc aacgtgatat tcaaatttta ggtattggtg
     2821 aaaatggtca tattggattt aatgaacctg gtacgccgtt tgatagcgtt actcatatcg
     2881 ttgatttgac tgaaagtact attaaggcta atagtcgata ttttaaaaac gaagatgatg
     2941 ttccaaagca agccatttcg atgggacttg ctaatattct tcaagccaaa cgtatcattt
     3001 tactcgcatt tggtgaaaag aaacgtgctg ctattacaca tttattaaat caggaaattt
     3061 ctgttgatgt tccagccaca ttacttcaca aacacccgaa tgttgagata tatttagacg
     3121 acgaagcttg cccgaaaaat gttgcgaaaa ttcatgtcga tgaaatggat tgattgcaat
     3181 gtttaattaa gaaatgcctc gggaaaggtt ccaatagaaa gataaaaagc attggaagga
     3241 tgatttttag tggaattaca attagcaatt gatttattaa acaaagaaga cgcggctgag
     3301 ttagcaaata aagtaaaaga ttatgtagat atcgtagaaa tcggtacgcc aatcatttac
     3361 aacgaaggtt taccagcagt taaacatatg gcagacaaca ttagtaatgt aaaagtatta
     3421 gcagacatga aaattatgga tgcagctgat tatgaagtta gccaagcaat taaatttggc
     3481 gcggatgtaa ttacaatact aggtgttgca gaagatgcat caattaaagc agctattgaa
     3541 gaagctcata aaaataataa acaattacta gttgatatga ttgctgttca agatttagaa
     3601 aaacgtgcaa aagaactaga tgaaatgggt gctgattata ttgcagtaca cactggttat
     3661 gatttacaag cagaagggca atcaccatta gaaagtttaa gaaccgttaa atctgttatt
     3721 aaaaattcta aagttgcagt agcaggtgga attaaaccag atacaattaa agatattgtc
     3781 gctgaaagtc ctgatcttgt tattgttggt ggcggaatcg caaatgcaga tgatccagta
     3841 gaagctgcaa aacaatgtcg cgctgcaatc gaaggtaagt aatatggcta aatttagtga
     3901 ctatcaatta attctagatg aattaaagat gactttgtca catgttgaag cggatgagtt
     3961 ttcaactttt gcatccaaaa tactacatgc tgaacatata tttgtagctg gcaaaggacg
     4021 ttcaggattc gtggcgaata gttttgcaat gcgcttaaat cagctcggca aacaggcaca
     4081 tgttgttgga gaatcaacga cacctgcgat taagtcgaat gatgtatttg taattatctc
     4141 tggttcaggt tccacggaac atttaagatt attagcagac aaagcaaaat cagtaggtgc
     4201 tgacatcgta ttaattacta caaataaaga ttctgcaata ggcaatctag ctgggacgaa
     4261 catcgttttg cctgcaggta caaaatatga tgaacaaggc tcggcacaac cattaggaag
     4321 tttgtttgaa caagcatctc aattattttt agatagtgtt gtaatgggat tgatgactga
     4381 aatgaatgtt acggaacaaa cgatgcaaca aaatcatgct aatttagaat aaaataaaga
     4441 tagtcgataa tatgatgcct aggcagaaat attatcgatt atttttttat ttaaataata
     4501 aattatagta taatatcaat aataaacgaa taggggtgtt aatattgaag tttgacaatt
     4561 atatttttga ttttgatggt acgttggcag acacgaaaaa atgtggtgaa gtagcaacac
     4621 aaagtgcatt taaagcatgt ggcttaacgg aaccatcatc taaagaaata acgcattata
     4681 tgggaatacc tattgaagaa tcatttttaa aattagcaga ccgaccatta gatgaagcag
     4741 cattagcaaa gttaatcgat acatttagac atacatatca atctattgaa aaggactata
     4801 tttatgaatt tgcgggtata actgaagcca ttacaagttt gtataaccaa gggaaaaaac
     4861 ttttcgtggt gtctagtaag aagagtgatg tattagaaag aaatttatcg gctattggat
     4921 taaatcactt gattaccgaa gctgttggat ccgatcaagt aagtgcatat aaaccaaatc
     4981 ctgaaggcat acacacaatt gtgcaacgct acaatttaaa tagccaacaa acggtttata
     5041 ttggtgattc aacatttgat gttgagatgg cgcaacatgc tggtgtacca tctgcagcag
     5101 taacatgggg agcacatgat gcaaggtcat tacttcattc aaatccagat tttataatca
     5161 atgatccatc agaaattaat acagtattat aaaatttgtt aaaacagaga ataccatggt
     5221 taaaatgcat attcataaat attagattat acatagaaat atttcgcttt agattaggaa
     5281 tgtaaaataa atatttatta ataatcgtgt atgtttaaag agtgatgtct gattcgttga
     5341 tcatttattt ttgtacaaat aaattaaagt aaaaacaaag ttattgaagt aaatcgaata
     5401 taaacatttt aatatgatga ggaaagtagt gatagcatat gacttacatg aagttgtgtg
     5461 ctatcgctat ttttagttat aattccaaaa agttaatcgt tcgatgattt aagaattatt
     5521 attgtttaat tcaaatgtat gagggtataa aatcattgaa tttaattcga taaagtgaaa
     5581 tttttgaaca aacatacttt tgtatttata taaaagttta aattcttata aatttgacaa
     5641 aactaattaa ctccgtataa ttatgaaaca tacaagaggg agtgtatgaa ttcatggatt
     5701 ttaataaaga gaatattaac atggtggatg caaagaaagc taaaaaaacc gttgttgcaa
     5761 ccggtatcgg taatgcaatg gaatggttcg attttggtgt ctatgcatat acaactgcgt
     5821 acattggagc gaacttcttc tctccagtag agaatgcaga cattcgacaa atgttgactt
     5881 tcgcagcatt agccattgcg tttttattaa gaccaattgg tggtgtggta tttggtatta
     5941 ttggtgacaa atatggacgt aaagttgtat taacatctac aattatttta atggcatttt
     6001 caacattaac cattggatta ttgccaagct atgatcaaat tggactttgg gcaccaatac
     6061 tattattgct tgcaagagta ctacaagggt tttcaacagg tggagagtat gcgggggcaa
     6121 tgacatatgt tgcggagtca tctccagata agcgtcgtaa ctcattaggt agtggactag
     6181 aaattgggac attatcaggt tacatagctg cttcaattat gattgcagta ttaacattct
     6241 ttttaacaga tgaacaaatg gcatcatttg gttggagaat cccattctta cttggtttat
     6301 tcctaggatt attcggctta tatttacgtc gtaagcttga agaatcacca gttttcgaaa
     6361 atgatgttgc aacacaacca gaacgagaca acattaactt tttacaaatc atcagatttt
     6421 attacaaaga tatatttgtg tgttttgtag ctgttgtatt cttcaatgtt acgaactata
     6481 tggtaactgc atatttacca acctatttag aacaagttat taaattagat gcaacgacaa
     6541 caagtgtatt aattacttgt gtcatggcaa taatgattcc attagcatta atgtttggta
     6601 agttagcgga taaaataggt gaaaagaaag tatttctaat tggtactggt gggctaacat
     6661 tatttagtat catcgcattt atgttattac attcacaatc atttgttgta atagtaatcg
     6721 gtatatttat attaggattt ttcttatcaa cttacgaagc gacaatgcca gggtcgttac
     6781 caacgatgtt ttacagtcat ataagatatc gaactttatc agtaacattt aatatctctg
     6841 tttcgatatt tggtggtacg acgccattag ttgcaacatg gttagttacg aaaactggag
     6901 atccattagc acctgcgtat tatttaacag caatcagtgt tattggcttt ttagttatta
     6961 cattcttaca tttaagtaca gcaggaaaat ctctaaaagg ttcgtatcca aatgtagata
     7021 acgagcaaga tagagcttat tatgcagaac acccaaaaga agcattatgg tgggttaaag
     7081 aacgtaagaa ttagagattt taataaaaag tataaatcaa tcgtatataa gcactttaaa
     7141 gctagtaggt tctgctaact ttaaagtgct ttttaaattg agaactgtaa ttagccgtaa
     7201 taaagttttt gtatatacat aaacccccac tgcaatgatt atcgcaatgg gggaaagagg
     7261 ggacttaaag catatgttta gctttgaata cttaaaattc tcttgctatt gaaatgttag
     7321 gatgtaaata tgtcttagag tattttgtcc aacgcaatta atattgagac tctaaccttc
     7381 aatattatta tagagaacac aaacttaaat agattgggtg acttatttgt gtcagttatt
     7441 gcgattgcga taacttcttt tctctatata catatagtaa cgtcttatct aataaaaaac
     7501 atggtactac agtatcaaat ttatctaggg cttaagtttg atttttataa taggcaggtt
     7561 tacctgataa aaatacttat tcattatata atgttaacaa gatgtatttt aaagtttaca
     7621 ttgagtgagg gatattgatg aacgtaattt tagaacagtt gaaaacacat actcaaaata
     7681 aacctaatga catagcatta catatcgatg atgaaacaat tacatatagt caactaaatg
     7741 cccgcatcac tagcgcagtt gaatctttgc agaaatattc acttaaccct gtcgttgcta
     7801 ttaatatgaa atcaccggtg caaagtatta tttgttattt agctttgcat cgtttacata
     7861 aagtgcctat gatgatggaa ggtaaatggc aaagtactat acatcgtcaa ttgattgaaa
     7921 aatatggtat taaagatgta attggagata caggtctcat gcagaatata gactcaccga
     7981 tgtttattga ttcaacgcaa ttacagcact accccaattt attacatatt ggttttactt
     8041 cagggacaac tggactgcca aaagcatatt atcgtgatga agattcatgg ttggcttctt
     8101 ttgaagttaa tgaaatgttg atgttaaaaa atgaaaatgc aatagcagcc cctggaccac
     8161 tatcgcactc gttaacatta tatgcgttat tgtttgcttt aagttccggt cgtactttta
     8221 taggacagac cacttttcat cctgaaaagt tacttaatca atgtcataaa atatcatcat
     8281 acaaagttgc tatgtttctt gttccaacga tgattaaatc attattgtta gtttacaaca
     8341 atgaacatac aatccaatca ttttttagca gtggagataa gctgcattct tctattttta
     8401 aaaagataaa aaatcaagca aatgacataa atttgattga attttttggt acatcggaaa
     8461 ccagttttat cagctataac ttgaatcagc aagcaccagt tgaatcagta ggtgtgctat
     8521 ttccaaatgt ggaattgaaa acaacgaatc acgatcacaa tggtatagga actatttgta
     8581 taaaaagtaa tatgatgttt agtggctatg taagtgaaca atgtataaat aatgatgaat
     8641 ggtttgttac taatgataat ggctatgtaa aagagcagta tttatattta acgggacgtc
     8701 aacaggatat gttaattatt ggtggtcaaa atatatatcc agcacatgtt gaacgccttt
     8761 taacgcaatc ttcgagcatt gatgaagcaa ttatcatcgg tattccaaat gagcgttttg
     8821 gtcaaatagg cgtattgctt tattctggtg atgtgacact tacacataaa aatgtaaaac
     8881 aatttttaaa aaagaaagtg aaacgctatg aaattccatc gatgattcat catgtagaaa
     8941 agatgtatta cactgcaagt ggtaaaattg ctagagaaaa aatgatgtcg atgtatttga
     9001 gaggtgaatt ataatatgaa tcaagcagtc atagttgcag ctaaacgaac tgcatttggg
     9061 aaatatggtg gcactttaaa acatttagag ccagaacaat tgcttaaacc tttattccaa
     9121 cattttaaag agaagtatcc agaggtaata tctaaaatag atgatgtagt tttaggtaat
     9181 gttgttggga atggtggcaa tattgcaaga aaagcattgc ttgaagcggg gcttaaagat
     9241 tcaatacctg gcgtcacaat cgatcggcaa tgtgggtctg gacttgaaag tgttcaatat
     9301 gcatgtcgca tgatccaagc cggagctggc aaggtatata ttgcaggtgg tgttgaaagt
     9361 acaagtcgag caccttggaa aatcaaacga ccgcattctg tgtacgaaac agcattacct
     9421 gagttttatg agcgtgcatc atttgcacct gaaatgagcg acccatcaat gattcaaggt
     9481 gctgaaaatg tggccaagat gtatgatgtt tcaagagaat tacaagatga atttgcttat
     9541 cgaagtcatc aactgacagc ggaaaatgta aagaatggaa atatttctca ggaaatatta
     9601 cctataaccg ttaaaggaga aatattcaat actgatgaaa gtctaaaatc acatattccg
     9661 aaagataact ttggccgatt taagcccgta atcaaaggtg ggaccgttac cgctgcgaat
     9721 agttgtatga aaaatgatgg tgcagtttta ttgcttatta tggaaaaaga tatggcatac
     9781 gaattaggtt tcgagcatgg tttattattt aaagatggtg ttacggtagg tgttgattct
     9841 aattttcctg gcattggtcc agtaccagcc atttccaact tactaaaaag aaatcaatta
     9901 acgatagaaa atattgaagt cattgaaatt aacgaagcgt tcagtgcaca ggtagttgcc
     9961 tgccaacaag ctttaaatat ttcaaatacg caattaaata tatggggtgg tgcattagca
    10021 tcaggtcatc catacggtgc aagcggtgcc caattagtga ctcgattatt ttatatgttt
    10081 gacaaagaga ctatgattgc atctatgggg atagggggag gtctaggaaa tgcagcatta
    10141 tttactcgat tctaaccagc gattaaatgt gtcattttct aaggatagtg tggctgcata
    10201 ttatcagtgt tttaaccaac cttatagaaa agaagtacca ccattaatgt gtgcgtcatt
    10261 atggccaaaa tttgatttat ttaaaaaata tgcaaatagc gaactgattt taacaaaatc
    10321 agcaattaat caaactcaaa agatagaagt agacacaata tatgtagggc atttagaaga
    10381 tattgaatgc cgacagactc gcaatatcac acgttataca atggctttaa cattaactaa
    10441 aaatgatcaa catgtcataa cggttacaca aacttttatt aaggcgatga agtagagatg
    10501 aagtttaatg agatatggat aaatgaatat ttggcgctcg taaatgatga taatccaata
    10561 cataatgaga ttgtgccagg acaattagtg agtcaaatga tgctgatggc tatgtcatta
    10621 gagacaaacc agtgtcaaat taactacgtt aaacctattt taataaatga aaatatcgaa
    10681 ttcattgaac aacacgaaca cgaaattata gcaattaatg acgatggaga gattaaaata
    10741 aaaatttctt tgagcacaaa aaaataaccg atattagctg catgaacgca tattaattag
    10801 gagatgaaag gacagctaat atcagttatg tattgttatt attattggga acagagatga
    10861 atataggtta cgtttctttc tttgcacggg gatgcattaa tctaaaataa taataacaac
    10921 tatatcaatg tttaataaat tctggattat tggaacgatt agtcaattta actaactttc
    10981 atatgatcta tatcgtcttg taataaagag agcaatttga atatttcagt atcactaaat
    11041 gaatcgtcac atttaattga aacatgctga aacgttttgg ttataatttc ataaactggt
    11101 gcgccttcat ggtgatactg tcgataaata atcataacct atattacctc ctttgctact
    11161 ctatggttat attataaata acatttttat gtgtgacatc aaccttaagt atcaactttt
    11221 tatcagacat agaacgtatg atttactaag actatttatg tataaaagtt ctaaa
//
LOCUS       DANBWH010000078        11274 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.081, whole genome shotgun sequence.
ACCESSION   DANBWH010000078 DANBWH010000000
VERSION     DANBWH010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11274
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.081"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..674
                     /locus_tag="PPY92_001408"
     CDS             <1..674
                     /locus_tag="PPY92_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625419.1"
                     /translation="SIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKSKNIEVSQ
                     MTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEANDKNAYGY
                     IANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKNYVEYKAE
                     KVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAVCGTAFAA
                     GCSVG"
     gene            complement(842..991)
                     /locus_tag="PPY92_001409"
     CDS             complement(842..991)
                     /locus_tag="PPY92_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625420.1"
                     /translation="MKIKTFATFTHRQLDKQVNRFLKNDIHVLEIQTHSNWLYISAIV
                     FYEDK"
     gene            complement(1538..1854)
                     /locus_tag="PPY92_001410"
                     /pseudo
     CDS             complement(1538..1854)
                     /locus_tag="PPY92_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002483623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
     gene            complement(1847..2830)
                     /locus_tag="PPY92_001411"
     CDS             complement(1847..2830)
                     /locus_tag="PPY92_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDJ2625421.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILR
                     DRLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGL
                     NKPNILYLMKPIVTERDIYKIEKEENDVEPYGEKDVRFSNVQKFENRTSRSSKIERPE
                     VRKSNTNDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNFDDKEMLLQ
                     EFPEQLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEILTSLKRLK
                     KAIHDRGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ"
     gene            3256..3999
                     /locus_tag="PPY92_001412"
     CDS             3256..3999
                     /locus_tag="PPY92_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDJ2625422.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDI
                     EKIIRRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEK
                     QESRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTST
                     NTRQNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPA
                     NLNDNDSDIEEKSEETEPKKGFWSRLFGS"
     gene            complement(4215..4400)
                     /locus_tag="PPY92_001413"
     CDS             complement(4215..4400)
                     /locus_tag="PPY92_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625423.1"
                     /translation="MFGDQRQEATKYVIKEGYQDIYFLNKNGEWYYFEVRSAWRGKHI
                     IRVKDGLLGWRKEIVTE"
     gene            4549..4644
                     /locus_tag="PPY92_001414"
     CDS             4549..4644
                     /locus_tag="PPY92_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDJ2625424.1"
                     /translation="MNEILVHIMTTAISGCLVTLFGYWLHKRDKK"
     gene            complement(4959..5198)
                     /locus_tag="PPY92_001415"
     CDS             complement(4959..5198)
                     /locus_tag="PPY92_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDJ2625425.1"
                     /translation="MKLAEAIKEQRELKRWSQEELANILKVSRQSVSKWESAKNYPSL
                     DILIAMSDLFGISLEHLIKGDARFKKVILEGNYRE"
     gene            complement(5285..6145)
                     /locus_tag="PPY92_001416"
     CDS             complement(5285..6145)
                     /locus_tag="PPY92_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDJ2625426.1"
                     /translation="MTGETVVYKNEMNLVPLRRFTATEINLFFAMCNKLKEQDTNTLR
                     LSFDELKKLSNYSPETRNINRFANDLDNVYKKMLNLTIRYEDDDVIERFVLFNHYRIH
                     KREQYLEISTSSNLKHILNSITNNFTKFELKEMTRLKSTYSKNMFRLLKQYKHTGYLK
                     IHIDDFKNRLDIPKSYRMTDINKNVFKPIIIELGSIFNNLTINKIKAKKGRKIEWIEF
                     TFDAEKRIHNKRQPQMSKIDKSRQYVRREKTPKWLEERSYEKQPQKDYDPQLEKERED
                     FLKQLELNWE"
     gene            complement(6509..7009)
                     /locus_tag="PPY92_001417"
     CDS             complement(6509..7009)
                     /locus_tag="PPY92_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HDJ2625427.1"
                     /translation="MYEIKTKNVGGWFHKEKQETGNIVITKTYFEKYTKQIKAAQMIL
                     DDYEWIKSGKSLKKSEKQNESLVNELTSVHMENEKLVEEFNDLAQRYNYLLSENEKKD
                     KELNYTLKLFNQVFKIIKSMMKEERYHTLINHIDNHLDNSKIREVMTIDNNDEQFFKK
                     KYQAQE"
     gene            complement(7076..7402)
                     /locus_tag="PPY92_001418"
     CDS             complement(7076..7402)
                     /locus_tag="PPY92_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HDJ2625428.1"
                     /translation="MEEEYGKENLLYATVHMDEITPHMHYGVIPITKDGRLSAKEVVG
                     NKKALTEFQDRFNTYINKQGYDLKRGISRQLTKEKHDQVSGYKQKTEYHKQMYMREKQ
                     IEDHLK"
     gene            complement(7342..7719)
                     /locus_tag="PPY92_001419"
     CDS             complement(7342..7719)
                     /locus_tag="PPY92_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001643491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HDJ2625429.1"
                     /translation="MSYSIIRVVKVKSKTNTRGIQRENKNYENIDIDLSKSYLNYDLV
                     NDTKFDFNKKIDEKIEKNYKGKRKIRTDAIKHIDGLITSDNVFLINYLKKRQNSFLSM
                     LKRSWKKNTVKKIFYMRQYIWMR"
     gene            complement(7957..8583)
                     /locus_tag="PPY92_001420"
     CDS             complement(7957..8583)
                     /locus_tag="PPY92_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDJ2625430.1"
                     /translation="MIKLENVFVKKGNKNILDGCNFNFEKGKSYALVGESGAGKSTLL
                     NIIAGFEDVSQGSIYIEDKLLKKKVDFYRYTLGYLFQNFALLENQTISQNLDLALKFK
                     KNKKDNMNLKKEVLKKVGLDLDIKRIVSSLSGGEQQRVALARLILKDPKIILADEPTG
                     SLDTKNGKIVIDLLLKLLDENKTMIVVTHDLELAKRFDVIVNISELRS"
     gene            complement(8595..8906)
                     /locus_tag="PPY92_001421"
     CDS             complement(8595..8906)
                     /locus_tag="PPY92_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDJ2625431.1"
                     /translation="MKRTLIIVTIVLLIIILSLLLVRNEHLDRFNPLLKEKVSYAKVP
                     KDTQDYRNITVYSKNGEKKSYKLDFLGYDPTKEYVKVNHKGKYVRSIEYITKDIPSFL
                     K"
     gene            complement(8910..10844)
                     /locus_tag="PPY92_001422"
     CDS             complement(8910..10844)
                     /locus_tag="PPY92_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HDJ2625432.1"
                     /translation="MKKFKLFLDFSTLLLISGLLFLFFFKENEEIIPESSNILTISNW
                     DKSNSKSKVLDVIESGAKNQNIQIIKSVKDFDNKKEFFVFNSKRNNSDFIRNKTSLLT
                     PSDLLNREIKGKYYIIGEHFNVEELTSELKKAGLTSEVEYINRNILFFELVIDNDLLV
                     PFIFLGVLYLLYFLYDRGYNLKFYAIQKLHGFSTTRIIFHNIHIKIIYWLVLTTIFFI
                     ANILIIHIANLELDFLMFIRRFIVLLFVFACITTLLWLISYSLLLKLSIPLTLKGKKG
                     YKFSIFMGTFTKCIMVLILSFLLAQNLNAYTKLKNIYDSEKIWQKLDNYYTLEISPNI
                     RNSEEKQILETNIYNLIKKSEQLGGLLLKNNNIANPDKNNYIPENGNVFFINNNFLNF
                     YKNINHDFKMIDNHSDKINVFIPPRLQYQKSEIQKNHQGWIDFQKQQNKKVDVQVLSK
                     NVSVFSFDRTTNLKFGYLDSPIVMLLTPDDLTGDFYLAAVSQGGYLFKDLDTLKQLLK
                     DNHLEEEISGITNYKDSVLNELNETKTKMIITIVTIIINVFILLIATVFETIQYFSWN
                     KKQLLLRKIHGYSLFSSNVRYLTISILLSIMLAYTTHILFGSKILLFIIMSIAIIQHL
                     LQIAYIKYLEKYFKDLMREI"
     gene            complement(10919..11212)
                     /locus_tag="PPY92_001423"
     CDS             complement(10919..11212)
                     /locus_tag="PPY92_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HDJ2625433.1"
                     /translation="MKKKFVSSCIASTILFGTLLGVTYKAEAATVHVAGGVWSHGIGK
                     HYVWSYYSHNKRNHGSTAVGKYSSFSGVARPGVQSKASAPKAWGGNKTFYSLH"
BASE COUNT         3889 a         1736 c         1460 g         4189 t
ORIGIN      
        1 tttcaataat ttccttgttc ctactggtat ttagcgcgaa tttttctaat gcttacgcaa
       61 atgaagaaca aaaaagttct ttactagaaa atcaaaaaga aaaattgaaa agcaaaaaca
      121 tcgaagtatc acaaatgacc aagaaagaaa taagttttct agaacaattg caaaataaaa
      181 atgaagatat gcaaaaattc gaaaagcaaa tgaaaaaaga aggatttaaa aaaataaagc
      241 tagaagacaa aaattctaaa ttaaaatatc tagctacaga agcaaatgat aaaaatgcat
      301 atggctacat agctaataac gcttataaaa atgaaaaaac aaaagaaatt gtagtttctg
      361 aaacaatata tgataactac aacaaaaaaa tcacaaaatt cattgccgaa aaaagaagta
      421 tgactgaaaa caaagaggga aaaatactta ttaacaaaaa ttacgttgaa tataaggctg
      481 aaaaagttga taaaaacaga ccggtaacat atgcaggatt caaatggaac ggcaaagcat
      541 tcgcttgtag tatggctgga ctttatgcat gtgctcacta ctgtggagta tgggcaatcg
      601 taaatccaat agccggcggt gtatgtgaag cagtttgcgg aactgcattt gcagctggat
      661 gctctgtagg ttgattttat tccaattgct ttattgacgt tgagcctcgg aacccttaac
      721 aatcccaaaa cttgtcgaat ggtcggctta atagctcacg ctatgccgac attcgtctgc
      781 aagtttagtt aagggttctt ctcaacatca ataaattttt tcggcataaa tacgatgttc
      841 cttatttatc ctcataaaaa acaatagctg aaatatataa ccaattactg tgtgtttgaa
      901 tttctaatac atgaatatca ttctttaaaa acctattaac ttgtttatct aattgacgat
      961 gagtaaaagt agcgaaagtt ttaatcttca tgatatctct ccttttataa aaatagcaat
     1021 ccttattaca atattaaaca cttttatttt ggattttaag aggcattttg aagacttaga
     1081 ggcaaatcat ataggttttg tacttaaaaa tctatacagt ctcttaaaac gcaaatatga
     1141 gccaaataaa tatatgctaa tttcacaaac aaaaatttca gcaaaatcag tgactaattt
     1201 tttagatact gcccatttac atgcaaattt aaaaattagc ataaaatact ttaaaacttc
     1261 cccatgtttt tctaaattcc tgcagggctt ttcttatcaa aaacttagaa attttaattt
     1321 aactctaaaa tctccgtaag agcataaggg aaatttgcta tcattcaaaa atgatagatc
     1381 tggcaaggcc agacattcca agggttttaa gttattttaa agtgatatca tacaaagctg
     1441 tagtgtcaca aaagtgtgac actacataca gcatttgtca caaaaataag acagcacttt
     1501 tttacagaaa aacaataaaa aaagacatca gttatcacta atgtcttaaa taataatttt
     1561 gcttttcgaa attccaaatt gcggtaattg aatgtgcttt ttttcttcgt ctgtcgtttt
     1621 atcttcatca cttgtattaa tcaaattacc atcttcggca tcgtctaagt ttaaaatttt
     1681 atgcttttgt ttaagcaatc caccataact taacatacgt tttcgataca aacctttttc
     1741 taaatcactt acaatttctt gatttttttc gtcgtcatca gttaaaaagt cagatgattt
     1801 aacggaatat tttgatgtct cttcgagtgt tataaaatca tttgaattat tgattaaaga
     1861 tattatattg tttcatgttt ttacggcgca tataaagggc atgtgtttct tcaagttcag
     1921 ttagaattgt ttgcattaaa taagcttgca ttgacttgat tgtttccttt tggcctttaa
     1981 ctcccctatc atgtatagct tttttaagtc tttttaaaga agttaatatt tcatcctcaa
     2041 tgtcctctaa catgtaatgc atatcttcat gccttgaatt aaatgatttt tttgctttaa
     2101 gaataacagc ctttatgatt tctaaatctc tataatcata ttttaataaa taattagtta
     2161 gttgttctgg gaattcttgc aatagcattt ctttatcatc aaaattatgt gtaaattgat
     2221 ttgaatgatt tgaatgatta ctaatttcat ttttgtactt tatatcattc aagtcattca
     2281 tatcattatt ctcagtatca ttaaagtcaa tgtcattaaa gtcagtatca ttagtgttcg
     2341 attttcgaac ttctggacgt tcgattttcg aacttctgga cgttcgattt tcgaacttct
     2401 ggacgttcga aaatcgaacg tccttctcgc cgtaaggttc aacatcgttt tcttcttttt
     2461 ctattttgta aatatcacgt tctgtgacaa taggtttcat taaatataga atatttggtt
     2521 tatttaaacc acgtcgtttt tgaatgagta gaccatcgtt ttctaaccct ttttttattt
     2581 ttgttaactt ttctttttta caatttaaga tttccattag cttttcattt gaataaacaa
     2641 agtaaatgtt accatcttca tcaacccagc tatttttaat agataaattt aagcgatcac
     2701 gtaagatagc ataggctatt ttcatatcat tagttagatt tttataattt tcactggtaa
     2761 agaatacttt agggatttga taaaatcttt ctttgtaatt ttcttgcact gtaaaatatt
     2821 gattttgcat gacaaaaaac ctctttccat attttagttt ggtaagaggt aataaaacgt
     2881 ttttatttag ctttacattt atttaatgat ttaggtgctt gcactaagat attattcatt
     2941 atataataca agtatcgaaa attatataaa taaacagttt ctaaacgttt tattaccaaa
     3001 ccccgactat taccagtagt tggggttttt gttatattaa gtttattata gcactttaaa
     3061 taaacttata aaagggaaaa tatagcttta tcaagggttt ataaaccctt gataaatatc
     3121 ataaaccttt cataaaccct tgataaaaag gttgatggat gtttattttt atttattaat
     3181 cactttatta ttaagcatta taagcttata atattttatc aagggtttat aaacatttta
     3241 taaagggttg atgatatgaa aagtgtgaaa aagttgagtg aagaattagg ggttagtaag
     3301 caaacgattt ttaataatat taagcgattg aatatagaga caataaaaca ggaaaacact
     3361 tcgtttatta aagaagatac cgatatagaa aaaatcattc gaagagtaaa tgaaaataaa
     3421 aagaaatatg gttttgaaag tactactgaa gacaaacaaa aaaaagggtc ggataatatt
     3481 aattttgaat cgaaaagcga tgctcaaata gtagaaatcc ttaaaaatca gattgatact
     3541 ttaaataatc aaattgaaaa acaagaaagt cgtcatgaaa caacaattga attttacaga
     3601 aaagagttgc aagaaagatc aaaattgctt gaaaatcagc aagttctggc attagagagt
     3661 aataaaaaga ttcaaaaatt agaaaatcaa ttagaaaaag aaaaacacct taattatccc
     3721 tttgatacat ctactaacac tagacaaaac gttgatgcac aagaaaaaac atatactact
     3781 aatcctgtaa atattaacag agatcaggaa gaaactaaag aaacagagat tcaatataag
     3841 gatatatctg gaagtcaaag cgacgaaagt acacaaggtg aagaagcaaa aaaagaagac
     3901 gttccagcta atctaaatga caatgatagt gatatagaag aaaagagtga agaaacggaa
     3961 cctaaaaagg ggttttggag tcgtttgttt ggtagctaaa ataattgatt aggcactaaa
     4021 agttataaaa ttttagtgtc ttttatatag tgagaacttc tggtaatcat cgattatgtt
     4081 aaccaaacaa aaagccataa atgccgtcat ataggcattt atggcttttt actttcatta
     4141 tcttttatgc atcttttata catcgttgat ttgacgcgtt ttcattcttt tgcattggca
     4201 agtattttgt atctttattc agttactatt tcttttctcc aacctaacaa accatcttta
     4261 actctaataa tatgtttgcc acgccaggca cttcttactt cgaaataata ccattccccg
     4321 tttttgttga ggaaataaat atcttgataa ccttctttaa tgacatattt tgtagcttct
     4381 tgtctttgat cgccaaacat tatcaatccc acccttatat ttatttatct ttaatatata
     4441 acaacattta ttaatattca tgttatttca tggaagaaga gatttgttat aatgtgaagg
     4501 tcgtctatct ctcagacgtc aattcggcgt agagaggagg tgcgatttat gaatgaaatt
     4561 cttgttcaca tcatgacaac ggcaatcagt ggttgtctcg ttacgttatt tggttattgg
     4621 ctccataaac gtgataaaaa ataatataga cgattaaagc caattcacca aaaaaccccg
     4681 aactacggcg aatagttcgg ggtttggtgc gtttacaaac gaaattcttg ttacttttag
     4741 tatatatttc atattgcctt ttgtaaactc ttctattctt atcaatacat ttcaaggtgt
     4801 cgtaaaaata ctttcctttt tcatattgcc tgatgtgcat taaattttga ttttaaaatc
     4861 aacccatgaa cgaatgaatg gcccaaacta aaaatccggc aacaggaaag aaaagccacc
     4921 aaaaatcata aagaaaatca actatggaag gtgccctgtt actctctata atttccctct
     4981 aaaataactt ttttaaaacg tgcatctcct ttaattaaat gctctaaaga gattccaaat
     5041 aaatcgctca tagcaatcaa aatatctaag gaaggatagt ttttggcact ttcccattta
     5101 gaaacacttt gtctggaaac ctttaatata ttggctaatt cctcttgaga ccatcttttt
     5161 aattctctct gttcttttat agcttcagct aatttcataa aatcactcct taactcaaac
     5221 tataattaaa ataattgaaa agtcacgata cttttagtta accaactcgc aacgaaaagt
     5281 tgcgttattc ccaattcagt tcaagttgtt ttaaaaaatc ctctcgttct ttctctaatt
     5341 gtgggtcgta atctttttga ggttgctttt catatgaccg ttcttctaac catttaggcg
     5401 tcttttcacg cctcacatat tgtcttgatt tatctatctt ggacatttga ggttgtcgtt
     5461 tgttatgaat gcgtttctca gcgtcaaatg tgaactctat ccattcaatt ttacgccctt
     5521 ttttcgcttt gattttattg atagtaagat tattaaatat agaacctaat tcaataataa
     5581 taggtttaaa aacatttttg ttaatatcag tcatacgata agatttaggt atatctaaac
     5641 gatttttaaa atcatcgatg tgaattttta aatagcctgt atgtttgtat tgtttaagta
     5701 atctaaacat atttttagaa tatgtagatt taagcctagt catttctttt aattcaaatt
     5761 ttgtaaaatt attagtaatt gaattcaata tgtgttttaa atttgaagaa gttgatattt
     5821 ctagatattg ttctcgctta tggattctat aatgattgaa taaaacgaat ctttctatta
     5881 catcatcatc ttcatatcta atggttaaat tcaacatctt tttatacaca ttatctaaat
     5941 cattagcaaa tctattaata ttacgtgttt ctggactata attacttaat ttttttaatt
     6001 catcaaaaga caaacgtaat gtattagtat cttgctcttt taatttatta cacatcgcaa
     6061 aaaataaatt aatttcagta gctgtaaatc gccttaatgg aactaaattc atttcatttt
     6121 tatatacaac tgtctctcct gtcatgatac agcccccctt tattacgaac ctaaaggagt
     6181 atcgtttgaa tatacactcc catttgaaaa cttttattca caataaacta ctttattatc
     6241 aatgtcaaac cataaaagga gtacaacttt ttaaaacact ccttttatgt ggcaaaaaca
     6301 ctcctttagg cattgaaatc ccttgttgct ctaggtctga acaagcccct aaaaaggttt
     6361 ttaaaaagat ttataaaaat atattaaaaa gttagagggg ggctacctat ttttcatcct
     6421 aaaaaataat taacctcaaa caaatgcatt taaatatcta tttttataat gtataaccat
     6481 cttctctatc ttctttaaaa atgatttctc attcttgtgc ttgatatttc ttcttaaaga
     6541 actgttcatc attgttatct atcgtcatta cttcacgtat tttagaattg tctaaatgat
     6601 tatcaatatg atttattagc gtatgataac gctcttcttt catcatactt ttaatgattt
     6661 taaaaacttg attgaataac ttaagcgtat aatttagttc tttatctttc ttttcattct
     6721 ctgacaacaa ataattataa cgttgtgcta aatcattaaa ttcttcaacc agcttttcat
     6781 tttccatatg gacactagtc aattcattaa ctaaactttc attttgtttc tctgattttt
     6841 ttaatgactt cccactttta atccattcat aatcatctaa tatcatctgt gctgctttaa
     6901 tttgtttagt gtatttttca aaataagttt tagtaattac aatattgcct gtttcttgct
     6961 tctctttatg aaaccatcca cctacatttt tagttttaat ttcatacatg acactcagaa
     7021 gctttttaag cgtatctaat gatttttggt agttttgtat caatttatca ttttctcact
     7081 ttaaatgatc ctctatttgc ttctcgcgca tatacatttg tttatgatat tctgtttttt
     7141 gtttatagcc gctaacttga tcatgttttt cttttgtaag ttgtctagaa attcctcgtt
     7201 ttaaatcata gccttgctta ttaatatacg tattgaatct atcttggaat tcggttaatg
     7261 cttttttatt acctacaact tctttcgcac ttaaacgtcc atctttagta ataggaatta
     7321 cgccataatg catatgtggt gttatctcat ccatatgtac tgtcgcatat aaaagatttt
     7381 ctttaccgta ttcttcttcc aagaacgctt tagcatgctt aaaaaactat tttgtctctt
     7441 cttcagataa ttgattaaaa acacattatc tgatgtaatt aatccatcaa tatgtttaat
     7501 ggcatctgtt ctaattttgc gtttcccttt ataatttttt tcgattttct catcaatttt
     7561 tttattaaaa tcaaacttag tatcattgac taaatcatag tttaaatatg acttacttaa
     7621 atcaatatct atattttcat aatttttatt ttctctttga atacctcttg tatttgtttt
     7681 tgatttcact ttaacaactc gaataattga ataagacata ataaatcaac ttccactgca
     7741 tatgattata acacctaaac acagaaaagt tttaacatac tagacttatt aaaaaccaaa
     7801 taagtcatta aaaacgtgtg ctctgcgagg cttgtttttt gtcatcacaa aaaaggactg
     7861 gaaaatccag tccttaaatt aaaaaactca aaagtaatga ctaatcatat ttcatattgt
     7921 tttttctcta tgtatggcta tacctatcaa tatttcttaa cttctaagct cagaaatatt
     7981 gactatgaca tcaaatcgtt tcgcaagctc taaatcatgc gttacgacaa tcatagtttt
     8041 attttcatca agtagcttca ataataaatc aataactatt tttccatttt tcgtatctag
     8101 tgatccagta ggttcatcag ctaaaatgat tttcggatct tttaatatta atcttgccaa
     8161 tgctactctt tgttgttcac caccgcttag agaactaaca attctcttga tatctaaatc
     8221 taggccgacc ttttttaata cttctttctt taagttcata ttatcttttt tattcttttt
     8281 gaactttagg gctaaatcta aattttgact gattgtttga ttttcaagta aagcaaaatt
     8341 ttgaaacaaa taccctaacg tatatctata aaaatcgact ttctttttta gtaacttatc
     8401 ttcaatatat atgcttcctt gtgatacatc ttcgaaccca gcgattatgt taagtaaagt
     8461 agattttcca gcgccacttt ctccaactaa agcatatgat tttccttttt caaaattaaa
     8521 gttacatccg tctaaaatgt ttttattacc tttttttaca aaaacatttt caagttttat
     8581 cactatcggt cacctcattt taaaaagcta ggaatatctt ttgtaatgta ttctatcgaa
     8641 cgcacatatt tccctttatg atttactttc acatattctt ttgtaggatc ataacctaaa
     8701 aaatccaatt tataactctt tttttctcca tttttagagt atacagttat atttctatag
     8761 tcctgggtat cttttggaac tttggcataa gatacttttt cttttaacag tgggttaaaa
     8821 cgatctaaat gctcatttct aaccaataac agactaagta tgatgattaa tagtacaatt
     8881 gttacaataa ttaaagttct tttcattgtt taaatctccc tcattaaatc tttaaagtat
     8941 ttttcaagat atttaatata agcaatttga agtaaatgtt gaataatagc aatagacatt
     9001 attatgaata gtaatatctt tgaaccaaat aaaatatgcg tagtgtaagc taacataata
     9061 gaaagtaaaa tagaaattgt taaataacgt acattactgc tgaataatga ataaccatga
     9121 atttttctta ataatagttg ctttttattc cagctaaaat attgaattgt ttcaaataca
     9181 gtagctatca aaagaatgaa aacgttaata ataatagtaa ctattgttat tatcattttt
     9241 gttttcgttt catttaattc atttaacaca gaatccttgt aattagttat accgctaatt
     9301 tcttcttcta ggtgattatc tttaagcaac tgtttaagtg tatctaaatc tttaaataga
     9361 tatccacctt gagaaacagc agctaaataa aaatctcctg ttaaatcgtc aggtgtcaac
     9421 aacattacta ttggagaatc tagatatcca aatttcaaat tagttgtgcg atcaaaagaa
     9481 aaaacagata cgttttttga cagtacttgc acatctactt ttttgttttg ttgcttttga
     9541 aagtcaatcc atccttggtg atttttttga atttcagatt tttgatattg taaccttgga
     9601 ggaataaaca cgtttatttt atccgaatga ttgtcaatca ttttaaaatc atgatttata
     9661 tttttataga aattcagaaa attgttattg ataaaaaaga cattgccatt ttcaggaatg
     9721 taattgtttt tatcgggatt ggctatatta ttattcttta aaagcaatcc accaagttgt
     9781 tcgcttttct taattaaatt gtatatatta gtttctaaaa tttgtttttc ttctgaattt
     9841 ctaatattag gagaaatctc taatgtatag taattatcta acttttgcca gattttttca
     9901 gagtcatata tattctttaa cttcgtataa gcatttaaat tttgtgccag taaaaaagat
     9961 agtattagca ccatgataca tttggtgaag gtacccataa atattgaaaa cttatatcct
    10021 tttttccctt ttaaggttaa tggaatactt aacttcaaaa gaagactata cgaaatcagc
    10081 cacaacaatg tagtaataca agcaaaaaca aaaagcaaca cgataaatct acgaataaac
    10141 attaagaaat ccaattctaa attggctata tgaatgatta atatattagc aataaagaaa
    10201 atagtggtaa gtactagcca ataaatgatt tttatatgaa tattatgaaa aataatgcgt
    10261 gtagttgaaa agccatgtag cttttgaata gcataaaatt tcaaattata tcctctgtca
    10321 tataagaagt acaaaagata taatacgcct aagaatatga aaggcactaa taaatcatta
    10381 tctatgacta attcaaaaaa taatatgttt ctatttatgt attcaacttc agatgttaaa
    10441 ccagcttttt ttaattcact cgtaagctct tctacattga aatgttcgcc aataatataa
    10501 tactttcctt ttatctctct gtttaataaa tctgatggag ttaaaagtga tgttttatta
    10561 cgtataaagt cagaattatt tcgttttgag ttgaaaacaa aaaattcttt tttattatca
    10621 aaatctttaa cggatttaat tatttgtata ttttgatttt tggcgcctga ttcaatgaca
    10681 tctaacactt ttgatttact gttagattta tcccaattag agattgttaa aatattggaa
    10741 ctttcaggta taatttcttc attttctttg aagaaaaata aaaataatag ccctgaaatt
    10801 aataataaag tactaaaatc taaaaataat ttgaattttt tcatattgcc tctttcaaga
    10861 gaatagacag aattaagttt acttaattct gtctattctc cttaattaat atatgtaatt
    10921 agtgaagact ataaaatgtt ttgtttccac cccaagcctt tggagcagat gccttagatt
    10981 gaacaccagg tcgagcaaca ccactaaaag atgaatattt ccctactgct gttgaaccat
    11041 gatttctttt attgtgacta taataagacc atacgtaatg ttttccaata ccatggctcc
    11101 aaacaccacc agctacatgt actgttgcag cttcagcttt ataagttaca cctaataaag
    11161 tgccgaataa gattgtacta gcaatacaac tagaaacaaa tttctttttc ataaaaacac
    11221 ctccatttaa tattatttat ttattatagc aaaaataaat aaaatagaga atga
//
LOCUS       DANBWH010000079        11245 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.082, whole genome shotgun sequence.
ACCESSION   DANBWH010000079 DANBWH010000000
VERSION     DANBWH010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11245
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.082"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            219..983
                     /locus_tag="PPY92_001424"
     CDS             219..983
                     /locus_tag="PPY92_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDJ2625434.1"
                     /translation="MFLDEHINRNFDKLNDNDLHIAHFINTHIDECKNMKIQDLAQFT
                     HASNATIHRFTRKLGFDGYSDFKSYLKFESNKTHQLPSNSIDSFKQEIDSTFSYLERV
                     DYQLLTEKMHTASTIYLYGTGRAQLNVASEAQRILLTLHNHVIVLHDEHELKMILNKS
                     EPNDLFFIISLSGETHELLEVTNLLQLRQKYFISVTTMKDNTLAQKANYNVYVSSNTF
                     YLTDGTDYSSFISYHIFFETLVRKYNEYLLHNDISN"
     gene            complement(1041..1547)
                     /locus_tag="PPY92_001425"
     CDS             complement(1041..1547)
                     /locus_tag="PPY92_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HDJ2625435.1"
                     /translation="MAKFNVENEHVEVEIEKLYKFSPELVYEAWTKKDLLKQWFMTSA
                     RTNKEIEADVKEGGKYRIVDQQRNGKVNVIEGIYESLVMDEYVKMTIGMPGLSETQDV
                     IEVEFFERETGGTQMLFYYRSLVEKERRFTNLEYKQKKKEYHDAMVHGFELMFDKMYH
                     VIETSTQQ"
     gene            complement(1714..3027)
                     /locus_tag="PPY92_001426"
     CDS             complement(1714..3027)
                     /locus_tag="PPY92_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HDJ2625436.1"
                     /translation="MEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITIT
                     VIVALLMNRKESFAKKVEVFTKGAGHSNIILMMLIFILAGAFSNTAEKMGGVKSTVNL
                     GLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVV
                     GGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISL
                     NHAKNYDYNLIKVVPYILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDA
                     VSKGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSV
                     ADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAA
                     GVAGISPLMLFPYSIYPILLGVCGLIAILFNFPKLKKNTSQEMKH"
     regulatory      complement(3104..3199)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(3252..3821)
                     /locus_tag="PPY92_001427"
     CDS             complement(3252..3821)
                     /locus_tag="PPY92_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625437.1"
                     /translation="MALIVSRAILFLILALAFGIATVFPEQKLPLYIVFTLFVAVAII
                     MNVWFTHQNVKKHIIKFNKYVAYFLSFVSGLLYIGFVIYTLTIGSYDVLPIIIFAISI
                     VLVQILENILGISIKNTRLSNKQLKVISKQRVQQFKVISLILVSVSIVFIFIALMLKW
                     WLLLVISIIVLIIITVAMIVYEKYYTTPD"
     gene            complement(4308..5189)
                     /locus_tag="PPY92_001428"
     CDS             complement(4308..5189)
                     /locus_tag="PPY92_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDJ2625438.1"
                     /translation="MATQDPRTKFKTTEYEKQEQEVPGLQSEMTPAPDCGETSYQGHQ
                     RLQGYKMLVTGGDSAIGRAAAIAYAKEGADVAINYLPSEEQDAQEVRQVIEESGQKAV
                     LIPGDIRDEQFNYDLVEQAYQQLGGLDNVTLVAGHQQYHDDIHGFTTEAFTETFETNV
                     YPLFWTVQKALEYLKPGASITTTSSVQGYNPSPILHDYAASKAAIISLTKSFSEELGP
                     KGIRVNCVAPGPFWSPLQISGGQPQSKIPTFGQKTPLGRAGQPVELCGTYVLLASEES
                     SYTTGQVFGVSGGVQID"
     gene            complement(5300..6421)
                     /locus_tag="PPY92_001429"
     CDS             complement(5300..6421)
                     /locus_tag="PPY92_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HDJ2625439.1"
                     /translation="MGLKDQAVTWRRYFHQFPELSDKEFKTTQKIKDILTEHHIRILD
                     LPLMTGLVAEVGQGLSCIAVRADIDALPIQELVEQDFKSENEGVMHACGHDIHMASIL
                     ATAVKLKEIEGTLTGRVKFIFQPAEELGHGAFKIIETDALKDVQAVLGFHNDPSRSVG
                     TFAIKTGAITSAVDRFEFHIKGVGGHAAKPEQCNDPVIVLAQLINSIQSIVSRNLSAF
                     DEAVVTIGQISCGNTWNVIADHAYVQGTVRSFDPVVRKLVETRLQDIADGLAQAYNMK
                     INLNYTHLPGAVMNDEALTHKAIAVAQHVGYKVEMMEQPLTIGEDFSGYSQHFPSVFA
                     LIGSHSEYDLHHPQYKPDERILEKVPEYFVEFVKRLLHE"
     gene            complement(6697..7935)
                     /locus_tag="PPY92_001430"
     CDS             complement(6697..7935)
                     /locus_tag="PPY92_001430"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HDJ2625440.1"
                     /translation="MNDLIINHIAELILPKSTDKPLKGKELDELNVVKNGTVVIKDGK
                     IVYAGQHTDDYDATETIDASGKVVSPALVDAHTHLTFGGSREHEMSLKRQGKSYLEIL
                     EMGGGILSTVNATRETSEDDLFKKAEHDLLTMIKHGVLAVESKSGYGLDRENELKQLK
                     VSNRLAEKYDLDMKHTFLGPHAVPKEASSNEAFLEEMIALLPEVKQYADFADIFCETG
                     VFTIEQSQHYMQKAKEAGFKVKIHADEIDPLGGLELAIDEQAISADHLVASSDKGKEK
                     LRNSDTVAVLLPATTFYLGKEDYADARGMLDNNGAIALATDYNPGSSVTNNLQLVMAI
                     AALKLKLSPSEVWNAVTVNAAKAIDINAGTINTGDKANLVIWDAPNHEYIPYHFGINH
                     AEKVIKDGKVIVDNTLSFKV"
     gene            complement(7935..9596)
                     /gene="hutU"
                     /locus_tag="PPY92_001431"
     CDS             complement(7935..9596)
                     /gene="hutU"
                     /locus_tag="PPY92_001431"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HDJ2625441.1"
                     /translation="MRKIQAKKGLSIECKGWEQEAVLRMLYNNLDPEVAERPEDLVVY
                     GGIGKAARNWEAFEAIEKTLRELESDETMLVQSGKPVAVFKTHEEAPRVLISNSVLVP
                     EWANWDHFNELDKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFAELGNQHFNGDLAGT
                     VTLTAGLGGMGGAQPLAITMNHGVAICVDVDETRVDKRIDTKYCDVKTADLDEALKLA
                     EEAKERGEGLSIGLVGNAVDIHQAILEKGFKIDIITDQTSAHDPLNGYVPQGYSVEEA
                     KVLREKDPKKYVELSQASMAKHVELMLEFQKRGAVAFDYGNNIRQVAFNNGVKNAFDF
                     PGFVPAYIRPLFCEGKGPFRFAALSGDPKDIERADEEMRKLFPENEKLLRWLDLAEEK
                     ISYQGLPSRIAWLGYGERAKMGLALNRLVRDGEISAPIVIGRDHLDAGSVASPNRETE
                     SMKDGSDAVGDWAVLNALINTAAGGSWISFHHGGGVGMGYSLHAGMVVVADGSERAER
                     RLERVLTTDPGMGVARHVDAGYDIAIQTAKEKGIHIPMIDKAGDK"
     gene            9756..10640
                     /locus_tag="PPY92_001432"
     CDS             9756..10640
                     /locus_tag="PPY92_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDJ2625442.1"
                     /translation="MKIIQLEYFLAIVKYNSFTKAAQFLHISQPSLTATIKKMEADLG
                     YDLFTRSTKDIKITEKGIQFYRYASELVQQYRSTMEKMYDLSVTSEPRIKIGTLESTN
                     QWIANLIRKHHSDYPEQQYRLYEIHDKHQSIEQLLNFNIHLAITNEKKTHEDIRSIPL
                     YEESYILLAPKETFKNQNWVDVENLPLILPNKNSQVRKHLDDYFNRRNIRPNVVVETD
                     RFESAVGFVHLGLGYAIIPRFYYQSFHTSNLEYKKIRPNLGRKIYINYHKKRKHSEQV
                     HTFVQQCQDYLYGLLEAL"
     gene            10822..11241
                     /locus_tag="PPY92_001433"
     CDS             10822..11241
                     /locus_tag="PPY92_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB1/FosB3 family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HDJ2625443.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
BASE COUNT         3612 a         2277 c         1590 g         3766 t
ORIGIN      
        1 acgacattga cgtattttga aagtattttg tataatcaga ttatcttttc atatagtgaa
       61 aattttttca cgaccttata tatgacatcg ttgtatttgt aatacattcg ttttaaacgc
      121 ataatcaaac ctatatcaat acacaaatat atataatgac atacaagatt ttaatgtaat
      181 aacgatctat cacacattta ttttcaagga ggttgaatat gtttttagat gaacacatta
      241 atcgaaactt tgataaactt aatgataatg atttacatat cgctcacttt atcaatacac
      301 atatagatga atgtaaaaat atgaaaatac aagatttagc gcaattcaca catgcctcga
      361 atgcaaccat tcacagattt acacgcaaat taggttttga cggttatagt gattttaaat
      421 cgtaccttaa atttgaaagt aataagacac accaattacc ttctaactct atcgatagtt
      481 tcaaacaaga aatcgatagt acattcagtt atttagaacg cgttgattac cagcttctta
      541 ctgaaaaaat gcatacagct tcaacaatat atttgtatgg tacagggcgt gcacagttaa
      601 acgttgcttc agaggcacag cgtatattgt taacgttaca taatcatgtc attgtacttc
      661 atgatgaaca tgaacttaaa atgattttga acaagagtga acctaatgat ttattcttta
      721 ttatttcatt atcaggtgaa acacatgaat tattagaagt aacaaacttg cttcaactta
      781 gacaaaaata ctttatttct gttactacaa tgaaagataa tacccttgct caaaaagcaa
      841 attacaacgt ctatgtatcc agtaacacat tctacttaac tgatggtaca gactattcta
      901 gcttcatcag ttatcatata ttctttgaaa cacttgtaag aaaatataat gagtatttac
      961 tacataatga tatttcaaat tagaaaaaag gaagttatga tttaatggcc ttgagcctat
     1021 cataacttcc ttttatcatt ttattgttgt gttgatgttt cgataacgtg gtacatctta
     1081 tcaaacatca attcgaaacc atgcaccatg gcatcatgat attctttttt cttttgcttg
     1141 tattctaaat tagtaaatcg tctttctttt tcaactaatg aacgataata aaatagcatt
     1201 tgggtgccac ctgtttcacg ttcaaaaaat tctacctcaa tgacatcttg tgtttcactt
     1261 agtccaggca taccgatagt catcttaacg tattcatcca taactaaaga ttcataaatg
     1321 ccttcaatca catttacttt gccattacgt tgttgatcta caatacgata tttaccgcct
     1381 tctttaacgt ccgcttcaat ctctttattc gttctggctg atgtcataaa ccattgtttc
     1441 aacaaatctt tctttgtcca agcttcgtat actaactctg gagaaaattt ataaagcttt
     1501 tcaatttcaa cttcgacatg ttcattctct acattaaatt ttgccactgt tgtccaccca
     1561 ctttcgctct tacttttatt ttaacgtatt tttgctcagt tccaaacata gatgatcatc
     1621 atttttaaaa gattagcgtt atacggtgag tacaacatga tctgttaata taacaagcca
     1681 ccttacttgg ctacatcgat atattgttaa gcattaatgt ttcatttctt gactagtgtt
     1741 cttttttagc tttggaaaat taaataaaat cgcaataagt ccgcatacac ctaataatat
     1801 aggataaatg ctgtatggga ataacattaa tggtgaaata ccagctacac cagccgctga
     1861 aatgacttgc gggctatatg gtaataaacc ttggaagcag cctccaaata tatcaagaat
     1921 acttgctgat ttccttgaat ctacatcata ttcatctgca atatttttag ctaaaggacc
     1981 tgacataata atagagatgg tgttgtttgc cgtggcaata tctgcgacac ttaccaaact
     2041 cgcaattcct aattctgcgc cacgctttga tttcacttta gagcgaacaa attgcaacaa
     2101 ccattcaata ccaccattgt gttgaataat accgactaaa ccaccaatta gcaacgcaat
     2161 catagcaata tcttccatgc ttataatacc tttggacact gcatctagta gtcccatcca
     2221 accgaatgaa ccatctatga gaccaatgat tccagctaat aatgttccgc caatcaatac
     2281 gataatgaca tttacaccta ataatgctaa taccaatact aaaatatacg gtacaacttt
     2341 aattagatta taatcatagt ttttagcatg atttaaagaa atgccattcg ttaagaaata
     2401 cagaataata atcgttaaaa tagcacctgg caatacaatt ttaaagttta ctctgaattt
     2461 atctttcatt ttcgtatgtt gtgttctaac cgcagcaatt gttgtatctg aaatcattga
     2521 tagattatcg ccgaacattg cacctccaac aactgtagcc attgctagcg cagttggtac
     2581 atctgtcgct tgtgcaaatc cgtaaccaac aggtgctatt gcagcaacag ttcctacaga
     2641 cgtccccata gatatagaaa caaacataca aatcacaaac aatcctacaa taattaaatt
     2701 ttctgggatt aatgatagtc ctaaattaac tgtcgacttt acgccaccca ttttttcagc
     2761 tgtatttgaa aatgcacctg ctaaaataaa aatcaacatc attaaaataa tgtttgaatg
     2821 gcctgcacct ttcgtgaaga cctcaacttt tttagcaaat gattcttttc gattcattaa
     2881 taacgccaca attaccgtta tcgtaattgc aacatttaat ggcattgaag taaaatcacc
     2941 tgtgataata cctacgccta aaaacaacgc cacaaataat aacaagggga ataatgccca
     3001 agcattgctc tttttatgta cttccatcct ttttacctgc tttccaatta aaaatacctc
     3061 tttctcacaa acgatgaaga aagaggtttt catgtgcttt acctgcttat cttcaaacca
     3121 ttacggttac tggaattggc acattcgaga tgttgccgag gcttcatagg gccagtccct
     3181 ccacctctct agataagtga tgcttattta cgtttacgtt acaagataat ccttagtacg
     3241 tcaatcataa attaatcagg agtcgtataa tatttttcat aaacaatcat tgctactgta
     3301 ataataatca aaacaataat gctaataaca agtaaaagcc accatttaag cattaatgca
     3361 ataaaaatga acacgataga cacacttact aatattaatg atatgacttt aaattgctga
     3421 acacgttgct tggagatgac tttcaactgt ttgtttgata gacgcgtatt ttttatactg
     3481 attcccagta tattttctaa tatttgaacc aatacgatac ttattgcaaa tataataatt
     3541 ggtaaaacat catagctccc tatagttaat gtataaatta caaatccaat gtaaagtaac
     3601 cctgagacaa aggataaaaa gtatgcgacg tatttgttaa acttaatgat atgcttttta
     3661 acgttttgat gggtaaacca tacattcata ataatcgcaa ctgctacaaa taatgtgaat
     3721 actatatata atggtaattt ttgttcagga aaaacagtcg ctattccaaa agctaatgct
     3781 aaaatcaaaa ataatatagc tctagatact attaatgcca taataacaac ccctttgttt
     3841 aatatcgagt ttgcaaattt acgtttatca gcatttctat gatcagtact tctacgggta
     3901 gcgtttctat gtaatttaca tcatcttaac atataaatac ttcgctattt aattgaaaac
     3961 atatcttatt attctttgtc cgttctgaca tttaatatct aaccttaggc atttcacttg
     4021 ttaataaatt taactttctt ccactaaccg tccctaaacc caatcccgca acagttttta
     4081 actttttcgt tgttgtcctg acatcctcat taagaaagtt tattctgctt aaaacttata
     4141 atccacaccc tgagcaaacg ctccttatga cagagtatta aaataagccg ataaagatac
     4201 acacctttac cgactattta aaatacactt caccaattca ttttaattta atggattgaa
     4261 gtaactaaat taatattatg ctgttcaatt aaaagcttca tacaacccta atctatttgc
     4321 actccaccgc taacaccgaa cacttgtcca gttgtataac ttgattcctc tgacgctaat
     4381 agcacataag ttccacataa ctcaacaggt tgacctgcac gacctaaagg tgttttttga
     4441 ccaaatgttg ggattttact ttgaggttgc ccaccagaaa tttgtaatgg tgaccagaat
     4501 ggaccaggtg ctacacagtt cactctaatt ccttttggtc ctaattcttc tgaaaaactt
     4561 ttagttaatg aaataattgc tgcttttgaa gcggcataat catgaagaat aggactagga
     4621 ttataacctt gtacagatga tgtcgttgta attgacgcac ccggttttaa atattccaat
     4681 gctttttgaa ctgtccaaaa tagcggatag acattcgttt caaatgtttc tgtaaatgcc
     4741 tcagttgtaa atccatgaat atcatcatga tactgttgat gtccagcaac taaagtaaca
     4801 ttatctaagc cacctaattg ttgatatgct tgttcaacaa ggtcatagtt gaactgctca
     4861 tctcttatat caccaggaat taacactgcc ttttgaccac tttcttcaat tacttggcgt
     4921 acttcttgtg catcttgttc ttcactcgga agatagttaa tcgctacatc tgcaccttct
     4981 ttagcatacg caattgctgc tgcacgccct attgctgagt caccacctgt gactaacatt
     5041 ttatagcctt gtaagcgttg atgaccttgg taagacgttt cgccacaatc aggtgctggc
     5101 gtcatttcag attgtaaacc cggtacctct tgttcttgtt tttcatattc cgttgtttta
     5161 aattttgttc taggatcttg agttgccatt ttttaacatc tccttattcg cttaatggtt
     5221 attatttacc caatcttcct aggaacttaa tcatgattac actaaaaatt actttcttct
     5281 ttataaaaac aagctcgaat tactcatgca atagtctctt tacaaattca acaaaatact
     5341 caggtacttt ttccagaatc ctttcatccg gtttatattg agggtgatgt aaatcatatt
     5401 cactatgaga accaattaac gcaaatacac ttggaaaatg ttgactataa cctgaaaaat
     5461 cttctccaat cgtaagcggc tgttccatca tttccacctt atatccaaca tgttgggcta
     5521 ctgcaattgc tttatgcgtc aatgcctcat cattcatcac agcgccaggt aaatgtgtat
     5581 aatttaaatt aattttcata ttatatgctt gagccaatcc gtccgcaata tcttgtaatc
     5641 gtgtttctac aagctttcgt accacaggat caaaactacg cactgtgcct tgtacatacg
     5701 catgatcagc aatgacattc caagtattac cacatgatat ttgtccaatt gttactaccg
     5761 cttcatcaaa cgcagataga tttctactaa ctatggattg aatactatta atcaattgcg
     5821 ccaacacaat aactggatcg ttgcattgtt ctggctttgc agcatgacca cccacgcctt
     5881 taatatgaaa ctcaaagcga tctactgctg atgtaattgc ccctgttttg attgcaaatg
     5941 tacctaccga acgcgatggg tcattatgaa aacccaatac tgcttgtaca tcttttaatg
     6001 catctgtttc aataatttta aaagcgccat gtcctagttc ttctgctggt tgaaaaatga
     6061 atttaacacg cccagtaaga gtgccctcaa tttcttttaa ttttacagcc gtagccaaaa
     6121 tactagccat gtgaatatca tgaccgcacg catgcataac accttcattt tcagatttaa
     6181 aatcctgttc aacaagctct tgaatcggta aagcatcaat gtcagcccga acagctatac
     6241 aactcagacc ttgtcccact tcagcaacca gcccagtcat aagcggtaag tctaatattc
     6301 taatatgatg ttctgttaaa atatctttaa ttttttgtgt agtcttaaat tctttatcgg
     6361 atagttctgg aaattgatga aaataccttc tccaggtaac agcttgatct tttaatccca
     6421 tcggtcattc cccttcctta agtcaatgat atgttgtcta ccctacgatg atcatctttg
     6481 actattaaac gatgatttca caacaatgta ctcttgttaa ttactttcgc taatgataga
     6541 cagttgttta ataatatcgt aacactgttg tcaaactatt ctaactttta taattaagaa
     6601 tctatacaaa aacgtgttct cggataaact tgtttttaca aaccacaaaa gctctaaaca
     6661 ttagtgtaaa ccaatgctta gagctttcta attattttat actttaaaag ataatgtgtt
     6721 atctacgatg accttaccgt ctttaataac tttttctgcg tgattgatac caaaatgata
     6781 tggaatatat tcatgatttg gtgcatccca aattactaaa ttagccttat cacctgtgtt
     6841 aattgtaccc gcgttaatat ctattgcttt agcagcgttg accgtaacag cattccacac
     6901 ttcactaggc gaaagcttta attttaatgc tgcaatggcc ataacaagtt gcaagttatt
     6961 cgttacactg ctgcctggat tataatcagt agctaacgcg atggcaccat tattatcaag
     7021 catacctctc gcatctgcat aatcttcttt acctaaatag aacgtcgttg caggtaagag
     7081 gacagctaca gtatcactat ttcgcaactt ttcttttcct ttatcactag aagctactaa
     7141 gtggtctgct gatattgctt gttcatcaat tgctaattcc agtccgccta acggatcaat
     7201 ttcatccgca tgtattttca ctttaaaacc tgcttctttg gctttttgca tataatgttg
     7261 cgattgttct attgtaaata cacctgtttc acagaaaata tccgcaaagt ctgcatattg
     7321 ttttacttcc ggaagtaacg caatcatttc ttctaaaaat gcctcatttg aacttgcctc
     7381 tttaggtaca gcatgaggcc ctaggaaagt atgtttcatg tctaaatcat atttctcagc
     7441 taaacgatta gacactttca attgcttcag ttcattttct ctatctaatc cataaccact
     7501 cttactttca actgcaagca cgccgtgttt aatcatagta agcaaatcat gctctgcttt
     7561 tttaaacaag tcatcttcgg atgtttctct agtagcatta acggtagata atatgccacc
     7621 acccatttct aatatttcaa ggtaagactt accttgacgt tttaatgaca tctcatgttc
     7681 tcgagatcca ccaaatgtta aatgggtatg agcatctact aatgccggag aaactacttt
     7741 tccactagca tctatcgtct cagtcgcatc atagtcatct gtatgttgtc cagcatatac
     7801 aattttgcca tctttaatga caactgtacc atttttcaca acatttaatt catctaattc
     7861 cttacccttc aaaggtttat ctgttgattt cggtaaaatt aattctgcta tgtgattaat
     7921 tattaaatca ttcattattt atcacctgct ttatcaatca ttggaatatg aatacctttt
     7981 tctttagctg tttgaatagc gatgtcatag ccagcatcaa catgtcgggc aacacccata
     8041 cctggatcag tcgtcaatac acgttccaat cttctttcag cacgctctga tccatctgct
     8101 acaacaacca tacccgcatg aagtgaatat cccatgccaa caccgccacc gtgatggaat
     8161 gaaatccatg aaccacctgc agctgtgtta atgagtgcat tcaatacagc ccaatcacca
     8221 accgcgtcac tgccatcttt catactttct gtttcacggt taggactagc aactgaacca
     8281 gcatctaaat ggtctcgtcc aataacaatt ggtgctgaaa tttcaccgtc acgtacaaga
     8341 cgatttaaag ctaagcccat tttcgctctt tctccatagc ctaaccaagc aatacgtgat
     8401 ggtagtcctt gatatgaaat tttttcttca gctaaatcaa gccatcttaa taacttttca
     8461 ttttctggga aaagtttgcg catttcttca tccgcacgct cgatatcttt tggatcacca
     8521 ctcaacgcag caaagcggaa tggcccttta ccttcacaga ataatggtct aatgtaagct
     8581 ggtacaaagc ctgggaagtc aaaagcattt ttcactccgt tattgaaggc tacttgacga
     8641 atattgttac cataatcaaa tgctacagcg ccacgttttt ggaattcaag cattaattca
     8701 acatgctttg ccattgaagc ttgtgacagt tcaacatatt ttttcggatc tttttcacgc
     8761 aatactttcg cttcttctac agagtatcct tgtggcacat atccatttag cggatcatgt
     8821 gcacttgttt ggtcagtaat aatgtcaatt ttaaatcctt tttctagaat cgcttgatgg
     8881 atgtctacag catttccaac taacccgatt gataatcctt ctccacgttc tttcgcctct
     8941 tctgctaatt ttaatgcttc atctaaatca gctgttttaa catcacagta tttcgtatca
     9001 attcgcttat caacacgtgt ttcatcaaca tccacgcaaa ttgctacccc atgattcata
     9061 gtaattgcta acggttgcgc accacccata ccacctaaac ctgctgtcag tgtaacagtg
     9121 cctgctaaat ctccattaaa gtgttgatta cctagctcgg caaatgtctc ataagtacct
     9181 tgcacaatac cttgagaacc aatatatatc caactaccgg ctgtcatctg tccatacatg
     9241 attaaacctt ttttatctaa ttcattaaaa tgatcccagt ttgcccattc aggcactaat
     9301 actgaatttg aaattaatac acgtggcgct tcttcatgtg ttttaaatac agcaactggc
     9361 tttcctgatt gtactaacat tgtctcatct gattctaatt ctcgtaacgt tttctctatt
     9421 gcttcaaaag cttcccaatt acgtgctgct tttccaatac caccataaac aactaaatct
     9481 tctggtcttt cagcaacttc tgggtctaaa ttgttgtata acattctaag tactgcttct
     9541 tgttcccaac ctttacactc aatactcaaa cctttttttg cttgaatttt tctcataaaa
     9601 ttcgctcctg ttcttttaag aagttaattc cactaaattt aaaacgctta cattattatc
     9661 ttcaatattc attatagtat gttaaaatat agccaacaaa tataaataaa ctaattatcc
     9721 atagcttgaa cctataaata aaaggagcaa aacacatgaa aattattcag ttagaatact
     9781 tcttggctat cgtgaaatat aatagtttta ctaaagctgc acaattttta catattagcc
     9841 agccatcttt aactgctacg attaaaaaaa tggaagcaga tttaggttat gacttattta
     9901 cacgttcaac aaaagacatc aagattaccg aaaaaggaat acagttttat cgttatgcga
     9961 gcgaattagt tcaacaatat cgatccacga tggaaaaaat gtatgattta agcgttacat
    10021 cagaaccaag gataaaaatt gggactcttg aatctacgaa tcaatggatt gcgaatttaa
    10081 ttcgaaagca ccattccgac taccctgaac agcaatatcg tttatatgaa atacatgaca
    10141 aacatcaatc tatagagcaa ttactgaatt ttaatattca tttagctata acaaatgaaa
    10201 aaaaaaccca cgaagatata agatccattc ctttatatga ggaatcttac attttattag
    10261 cacccaagga aacatttaaa aatcaaaatt gggtagatgt tgaaaatttg ccactcatat
    10321 taccaaacaa aaattctcaa gtgcgcaaac acttagatga ctattttaat agaagaaata
    10381 ttcgtccaaa tgtcgttgta gaaacagatc gattcgaatc agcagttgga tttgttcatc
    10441 tcggcttagg ttacgctatc attccgagat tttattacca atcatttcac acgtctaatt
    10501 tagaatataa aaaaattcgt ccaaacttag gccgaaaaat ttatatcaat taccataaaa
    10561 aacgcaaaca ctccgaacaa gtacatacat tcgtacaaca atgccaagat tatttatatg
    10621 gacttttaga ggctctttaa cttaagttat tagagcctct tatgcagttg cgtagtcaac
    10681 tgtatacctt tttcctttaa cttaagttat tagagcctct tatgcagttg ctcagatcat
    10741 cgtataaaaa ttaatgacgt catttcaaaa atcgatacaa aaataattta ttataaaaat
    10801 tctaagaaag aagtgaagca gatgttaaaa tctattaatc atatatgctt ttcagtcaga
    10861 aatttaaacg attcaataca tttttataga gatattttac ttgggaaatt gctattgact
    10921 ggtaaaaaaa ctgcttattt tgagcttgca ggcctatgga ttgctttaaa tgaagaaaaa
    10981 gatataccac gtaatgaaat tcacttttca tatacacata tagctttcac tatagatgac
    11041 agcgaattta aatattggca tcagaggtta aaagataata acgtgaatat tttagaagga
    11101 agagttagag atattagaga tagacaatca atttacttta ctgaccctga tggtcataag
    11161 ctagaattac atactggcac acttgagaac agattaaatt attataaaga ggctaaacca
    11221 catatgacat tttacaaata aggcg
//
LOCUS       DANBWH010000080        11245 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.083, whole genome shotgun sequence.
ACCESSION   DANBWH010000080 DANBWH010000000
VERSION     DANBWH010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11245
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.083"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            54..1742
                     /gene="ilvD"
                     /locus_tag="PPY92_001434"
     CDS             54..1742
                     /gene="ilvD"
                     /locus_tag="PPY92_001434"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDJ2625444.1"
                     /translation="MRSDMIKKGDHQAPARSLLHATGALKSPTDMNKPFVAICNSYID
                     IVPGHVHLRELADIAKEAIREAGAIPFEFNTIGVDDGIAMGHIGMRYSLPSREIIADA
                     AETVINAHWFDGVFYIPNCDKITPGMILAAMRTNVPAIFCSGGPMKAGLSAHGKALTL
                     SSMFEAVGAFKEGSISKEEFLDMEQNACPTCGSCAGMFTANSMNCLMEVLGLALPYNG
                     TALAVSDQRREMIRQAAFKLVENIKNDLKPRDIVTREAIDDAFALDMAMGGSTNTVLH
                     TLAIANEAGIDYDLERINAIAKRTPYLSKIAPSSSYSMHDVHEAGGVPAIINELMKKD
                     GTLHPDRITVTGKTLRENNEGKEIKNFDVIHPLDAPYDAQGGLSILFGNIAPKGAVIK
                     VGGVDPSIKTFTGKAICFNSHDEAVEAIDNRTVRAGHVVVIRYEGPKGGPGMPEMLAP
                     TSSIVGRGLGKDVALITDGRFSGATRGIAVGHISPEAASGGPIALIEDGDEITIDLTN
                     RTLNVNQPEDVLARRRESLTPFKAKVKTGYLARYTALVTSANTGGVMQVPENLI"
     gene            1770..3539
                     /gene="ilvB"
                     /locus_tag="PPY92_001435"
     CDS             1770..3539
                     /gene="ilvB"
                     /locus_tag="PPY92_001435"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HDJ2625445.1"
                     /translation="MSKTQHEVNQNIDPLKMAESLELEQLNEKTLNDMRSGSEVLVEA
                     LLKENVDYLFGYPGGAVLPLYDTFYDGKIKHILARHEQGAVHAAEGYARVSGKTGVVV
                     VTSGPGATNVMTGITDAHCDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNY
                     QVKRVEDIPKIVHEAFHVANSGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTE
                     PENKDIDTFISLLKEAKKPVVLAGAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVP
                     YEDTLFLGMGGMHGSYASNMALTECDLLINLGSRFDDRLASKPDAFAPNAKIVHVDID
                     PSEINKVIHVDLGIIADCKRFLECLNDKNVETIEHSDWVKHCQNNKQKHPFKLGEEDQ
                     VFCKPQQTIEYIGKITNGEAIVTTDVGQHQMWAAQFYPFKNHGQWVTSGGLGTMGFGI
                     PSSIGAKLANPDKTVVCFVGDGGFQMTNQEMALLPEYGLDVKIVLINNGTLGMVKQWQ
                     DKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFLIDKPEQLEEQLDAAFAYQGPVLIEVR
                     ISPTEAVTPMVPSGKSNHEMEGL"
     gene            3539..3793
                     /locus_tag="PPY92_001436"
     CDS             3539..3793
                     /locus_tag="PPY92_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDJ2625446.1"
                     /translation="MTRILKLQVADQVSTLNRITSAFVRLQYNIDTLHVTHSEQPGIS
                     NMEIQVDIQDDTSLHILIKKLKQQINVLTVECYDLVDNEA"
     gene            3915..4949
                     /gene="ilvC"
                     /locus_tag="PPY92_001437"
     CDS             3915..4949
                     /gene="ilvC"
                     /locus_tag="PPY92_001437"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDJ2625447.1"
                     /translation="MEEFIMTTVYYDQDVKTDALQGKKIAVVGYGSQGHAHAQNLKDN
                     GYDVVIGIRPGRSFDKAKEDGFDVFPVAEAVKQADVIMVLLPDEIQGDVYKNEIEPNL
                     EKHNALAFAHGFNIHFGVIQPPADVDVFLVAPKGPGHLVRRTFVEGSAVPSLFGIQQD
                     ASGQARNIALSYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGGVSKLIQSGFETL
                     VEAGYQPELAYFEVLHEMKLIVDLMYEGGMENVRYSISNTAEFGDYVSGPRVITPDVK
                     ENMKAVLTDIQNGNFSNRFIEDNKNGFKEFYKLREEQHGHQIEKVGRELREMMPFIKS
                     KSIENKIDLQ"
     gene            4963..6492
                     /locus_tag="PPY92_001438"
     CDS             4963..6492
                     /locus_tag="PPY92_001438"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDJ2625448.1"
                     /translation="MSSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIE
                     AGFPASSTGSFKSVQAIAQTLTTTAVCGLARCKKSDIDAVYEATKDAAKPVVHVFIAT
                     SPIHLEHKLKMSQEDVLASIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVD
                     AGATVINIPDTVGYSYHDEYAHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAI
                     EGGARRIEGTVNGIGERAGNAALEEVALALYVRNDHYGAQTALNLEETKKTSDLISRY
                     AGIRVPRNKAIVGQNAFSHESGIHQDGVLKHRETYEIMTPQLVGVSTTELPLGKLSGK
                     HAFSEKLKALGYNIDKEAQIDLFKQFKTIADKKKSVSDRDIHAIIQGSEHEHQALYKL
                     ETLQLQYVSSGLQSAVVVVKDKEGHIYQDSSIGTGSIVAIYNAVDRIFQKETELIDYR
                     INSVTEGTDAQAEVHVNLLIEGKTVNGFGIDHDILQASCKAYVEAHAKFAAENVEKVG
                     N"
     gene            6495..7541
                     /gene="leuB"
                     /locus_tag="PPY92_001439"
     CDS             6495..7541
                     /gene="leuB"
                     /locus_tag="PPY92_001439"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDJ2625449.1"
                     /translation="MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFG
                     GASIDTFGEPLTEKTLNACKRADAILLSAIGGPKWTDPNNRPEQGLLKLRKSLNLFAN
                     IRPTTVVKGASSLSPLKEERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTRE
                     EIERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDA
                     CSMHLITNPKQFDVIVCENLFGDILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGS
                     APDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYNMIEHGQTTADLGGKLN
                     TTDIFEILSQKLNH"
     gene            7555..8925
                     /gene="leuC"
                     /locus_tag="PPY92_001440"
     CDS             7555..8925
                     /gene="leuC"
                     /locus_tag="PPY92_001440"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDJ2625450.1"
                     /translation="MGQTLFDKVWNRHVLYGKLGEPQLLYIDLHLIHEVTSPQAFEGL
                     RLQNRKLRRPDLTFATLDHNVPTIDIFNIKDEIANKQITTLQKNAIDFGVHIFDMGSD
                     EQGIVHMVGPETGLTQPGKTIVCGDSHTATHGAFGAIAFGIGTSEVEHVFATQTLWQT
                     KPKNLKIDINGTLPTGVYAKDIILHLIKTYGVDFGTGYALEFTGETIKNLSMDGRMTI
                     CNMAIEGGAKYGIIQPDDITFEYVKGRPFADNFAKSVDKWRELYSDDDAIFDRVIELD
                     VSTLEPQVTWGTNPEMGVNFSEPFPEINDINDQRAYDYMGLEPGQKAEDIDLGYVFLG
                     SCTNARLSDLIEASHIVKGNKVHPNITAIVVPGSRTVKKEAEKLGLDTIFKNAGFEWR
                     EPGCSMCLGMNPDQVPEGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIHGKFVD
                     VRKVVV"
     gene            8926..9498
                     /gene="leuD"
                     /locus_tag="PPY92_001441"
     CDS             8926..9498
                     /gene="leuD"
                     /locus_tag="PPY92_001441"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDJ2625451.1"
                     /translation="MAAIKPITTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPF
                     AFDEWRYLPDGSDNPDFNPNKPQYKGASILITGDNFGCGSSREHAAWALKDYGFHIII
                     AGSFSDIFYMNCTKNAMLPIVLEKSAREHLAQYEEIEIDLPNQTVSSPDKRFHFEIDE
                     TWKNKLVNGLDDIAITLQYESLIEKYEKSL"
     gene            9513..10781
                     /gene="ilvA"
                     /locus_tag="PPY92_001442"
     CDS             9513..10781
                     /gene="ilvA"
                     /locus_tag="PPY92_001442"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HDJ2625452.1"
                     /translation="MTVKTTVSTKDIDEAFLRLKDIVKETPLQLDHYLSQKYDCKVYL
                     KREDLQWVRSFKLRGAYNAISVLSDEAKSKGITCASAGNHAQGVAYTAKKLNLNAVIF
                     MPVTTPLQKVNQVKFFGNSNVEVVLTGDTFDHCLAEALTYTSEHQMNFIDPFNNVHTI
                     SGQGTLAKEMLEQSKTDNVNFDYLFAAIGGGGLISGISTYFKTYSPTTKIIGVEPSGA
                     SSMYESVVVNNQVVTLPNIDKFVDGASVARVGDITFEIAKENVDDYVQVDEGAVCSTI
                     LDMYSKQAIVAEPAGALSVSALENYKDHIMGKTVVCVISGGNNDINRMKEIEERSLLY
                     EEMKHYFILNFPQRPGALREFVNDVLGPQDDITKFEYLKKSSQNTGTVIIGIQLKDHD
                     DLIQLKQRVNHFDPSNIYINENKMLYSLLI"
BASE COUNT         3761 a         1890 c         2170 g         3424 t
ORIGIN      
        1 tttttattac tataaaacat gattaatcat taaaatttac gggggaattt actatgcgaa
       61 gcgacatgat caaaaaagga gatcaccaag caccagcaag aagtctttta catgccacgg
      121 gcgcgctaaa aagtccaact gatatgaaca aaccatttgt agctatttgt aactcttata
      181 ttgatattgt tcctggacat gttcacttga gagagcttgc agatatagct aaagaagcaa
      241 ttagagaagc cggtgccatt ccatttgaat tcaatacaat tggtgttgat gatggaatag
      301 ctatgggaca tatcggaatg cgatattctc taccatcacg tgaaattatt gcagatgcag
      361 ctgaaactgt aattaacgct cattggtttg acggcgtatt ttacattcct aattgtgaca
      421 agattacacc cggtatgatt ttagcagcca tgaggacaaa cgtaccagct atcttttgct
      481 ctggtggacc aatgaaagct ggcttatctg cacatggaaa agcattaaca ctttcatcaa
      541 tgtttgaagc agtcggcgca tttaaagaag gatcgatttc taaagaagaa tttttagata
      601 tggaacaaaa tgcctgccct acttgtggtt catgtgctgg gatgtttact gcaaattcaa
      661 tgaactgttt gatggaagtt ttaggtctag cattaccata caacggtact gcacttgcag
      721 tcagtgatca gcgacgcgaa atgattcgcc aagcagcttt taaattagtt gaaaatatta
      781 aaaatgattt aaaaccacgt gatatcgtta ctcgcgaagc aattgatgat gcatttgcac
      841 ttgatatggc tatgggtggt tcaacaaaca cggtactgca tacgttagcc attgccaatg
      901 aagctggtat tgattatgac ttagagcgca ttaatgctat tgccaaacgc acgccatatt
      961 tatcaaaaat agcacctagt tcatcgtatt caatgcatga tgtgcatgaa gctggtggcg
     1021 tcccagcaat tattaatgaa ttgatgaaga aagatggcac gttacaccca gatagaatca
     1081 cagttactgg caaaacgtta cgtgaaaata acgaaggcaa agaaattaag aactttgatg
     1141 tcattcaccc tcttgatgca ccatatgatg cacaaggcgg tttatctatc ttatttggta
     1201 atatcgcccc taaaggcgca gttattaaag ttggcggcgt tgatccatct atcaaaacat
     1261 ttactgggaa agcaatttgt ttcaattcgc atgatgaagc tgttgaagca atagacaatc
     1321 gtaccgttcg tgcaggccac gtcgttgtca ttagatatga aggacctaaa ggtggaccag
     1381 gtatgcctga aatgttagca cctacttcct ctattgttgg tcgcggctta ggtaaagatg
     1441 ttgcattaat tactgatggg cgtttttccg gtgccacaag aggtattgca gttggtcata
     1501 tttcccctga agctgcatct ggtggaccaa ttgccttaat tgaagatgga gatgagatta
     1561 ctattgattt aacaaatcgt acattaaacg taaaccagcc tgaagatgtt ctagcgcgtc
     1621 gccgagaatc tttaacaccg tttaaagcga aagtaaaaac aggttatcta gctcgttata
     1681 ctgccctagt aactagcgca aatacaggtg gcgtcatgca agtccctgag aatttaattt
     1741 aatttatttt tatattggag atggttaaaa tgtctaaaac tcaacatgaa gtaaaccaaa
     1801 atattgaccc tttaaaaatg gctgaatcac ttgaacttga acaactaaat gaaaaaactt
     1861 taaatgacat gcgttcagga tcagaagtgc tagtagaagc tctacttaaa gaaaatgtgg
     1921 attatttatt cggttatcct ggtggtgccg tactaccttt atacgacaca ttttatgatg
     1981 gtaaaatcaa acatatttta gcaagacacg aacaaggtgc tgttcatgct gcagaaggtt
     2041 atgcacgtgt atctggtaaa actggcgtcg ttgtagttac aagcggtcca ggtgcaacta
     2101 atgtaatgac aggtattacg gatgcacatt gcgactcttt acctctagtt gtattcactg
     2161 gacaagttgc tacaccaggc attggtaaag atgcattcca agaagcggat attctatcta
     2221 tgacttcacc aattacaaaa caaaattatc aagtgaaacg tgttgaagat atccctaaaa
     2281 tcgtacacga agctttccat gtagctaatt ctggacgcaa aggtcctgta gtgattgatt
     2341 ttccaaaaga tatgggtgtt ttagctacaa atgtggattt atgcgacgaa atcaatattc
     2401 caggttatga agttgttaca gaaccagaaa ataaagacat tgacactttc atctcacttt
     2461 taaaagaagc gaaaaagcct gtcgtattag ccggcgcagg tattaatcaa tcaaaatcaa
     2521 atcaattatt aacacagttt gttaataaac atcaaatccc aacagttact actttactag
     2581 gtctaggtgc tgtaccttat gaggatacac tatttttagg tatgggaggt atgcatggtt
     2641 cttatgctag taacatggca ttaactgagt gtgatttact cattaattta ggtagccgct
     2701 tcgatgatag attagcaagc aaacctgatg cctttgcacc taacgccaaa attgtacatg
     2761 tagatattga tccttcagaa atcaataaag ttattcatgt agatttaggt attattgcag
     2821 actgtaaaag atttttagaa tgtttaaatg ataaaaatgt tgagactata gaacacagtg
     2881 actgggttaa acattgtcaa aataataagc agaaacaccc atttaaactt ggtgaagaag
     2941 atcaagtatt ttgtaagcca caacaaacaa tcgaatatat cggcaaaatt acaaatggtg
     3001 aagcaattgt tactacagac gtgggacaac atcaaatgtg ggcagctcaa ttttatccat
     3061 ttaaaaatca cggacaatgg gttacaagcg gtggtttagg aacaatggga ttcggtattc
     3121 cttcgtcaat tggtgccaaa ttagctaatc ctgataaaac agtcgtatgt ttcgtcggtg
     3181 acggtggttt ccaaatgaca aaccaagaaa tggcactttt acccgaatat ggtttagatg
     3241 tcaaaatcgt actaatcaat aatggaacat taggtatggt taaacaatgg caagataagt
     3301 tctttaatca acgcttctca cactcagtat ttaatggtca acctgatttt atgaaaatgg
     3361 cagaagcata tggcgtcaaa ggtttcttaa tcgataagcc agaacaactg gaagaacaat
     3421 tagatgcagc gtttgcttat caaggaccag ttttaattga ggttcgtatt tcccctactg
     3481 aagctgtaac cccaatggtt ccgagtggca aatcaaatca tgaaatggag ggcttataat
     3541 gacaagaatt cttaaattac aagttgcgga tcaagtcagc acgctaaatc gaattacaag
     3601 tgcttttgtt cgcctacaat ataatatcga tacattacat gttacacatt ctgaacaacc
     3661 tgggatttct aacatggaaa ttcaagtcga tattcaagat gatacatcac ttcatatatt
     3721 aattaaaaaa ttaaaacaac aaattaatgt tttaacggtt gaatgctacg accttgttga
     3781 taacgaagct taattttaag acaaaggcaa tgatgcgcta attagttata gatatatcat
     3841 aggctgctag ttaacatctg ccactattac aaagttatat ttcagaattt tcgaaaaaca
     3901 aaacatttaa ttatttggag gaatttatta tgacaacagt ttattatgat caagacgtaa
     3961 aaacggacgc tttacaaggc aaaaaaattg cagtagtagg ttatggatca caaggccacg
     4021 cgcatgcaca aaacttaaaa gacaatggat atgatgtagt catcggtatt cgcccaggtc
     4081 gttcttttga caaggctaaa gaagatggat ttgatgtgtt ccctgttgca gaagcagtta
     4141 agcaagctga tgtaattatg gtgctattac ctgatgaaat tcaaggtgat gtatacaaaa
     4201 acgaaattga accaaattta gaaaaacata atgcgcttgc atttgctcat ggctttaaca
     4261 ttcattttgg tgttattcaa ccaccagctg atgttgatgt atttttagta gctcctaaag
     4321 gaccgggtca tttagttaga cgtacatttg ttgaaggttc tgctgtacca tcactatttg
     4381 gtattcaaca agacgcttca ggtcaagcac gtaatattgc tttaagttat gcaaaaggta
     4441 ttggtgcaac tcgtgcaggt gttattgaaa caacatttaa agaagaaact gagacagatt
     4501 tatttggtga acaagcagta ctttgcggtg gtgtatcgaa attaattcaa agtggctttg
     4561 aaacattagt agaagcgggt tatcaaccag aattagctta ttttgaagta ttacatgaaa
     4621 tgaaattaat cgttgatttg atgtatgaag gcggtatgga aaatgtacgt tactcaattt
     4681 caaatactgc tgaatttggt gactatgttt caggaccacg tgttatcaca ccagatgtta
     4741 aagaaaatat gaaagctgta ttaactgata tccaaaatgg taacttcagt aatcgcttta
     4801 tcgaagacaa taaaaatgga ttcaaagaat tttataaatt acgcgaagaa caacatggtc
     4861 atcaaattga aaaagttggt cgtgaattac gcgaaatgat gccttttatt aaatctaaaa
     4921 gcattgaaaa taagatagac ctacaatgag gagttgttaa atatgagtag tcatattcaa
     4981 atttttgata cgacactaag agacggtgaa caaacaccag gagtgaattt tacttttgat
     5041 gaacgcttgc gtattgcatt gcaattagaa aaatggggtg tagatgttat tgaagctgga
     5101 tttcctgctt caagtacagg tagctttaaa tctgttcaag caattgcaca aacattaaca
     5161 acaacggctg tatgtggttt agctagatgt aaaaaatctg acatcgatgc tgtatatgaa
     5221 gcaacaaaag atgcagcgaa gccggtcgtg catgttttta tagcaacatc acctattcat
     5281 cttgaacata aacttaaaat gtctcaagaa gacgttttag catctattaa agaacatgtc
     5341 acatacgcga aacaattatt tgacgttgtt caattttcac ctgaagatgc aacgcgtact
     5401 gaattaccat tcttagtgaa atgtgtacaa actgccgttg acgctggagc tacagttatt
     5461 aatattcctg atacagtcgg ctacagttac catgatgaat atgcacatat tttcaaaacc
     5521 ttaacagaat ctgtaacatc ttcaaatgaa attatttata gtgctcattg ccatgacgat
     5581 ttaggaatgg ctgtttcaaa tagtttagct gcaattgaag gcggtgcgag acgaattgaa
     5641 ggcactgtaa atggtattgg tgaacgagca ggtaatgcag cacttgaaga agtcgcgctt
     5701 gcactatacg ttcgaaatga tcattatggt gctcaaactg cccttaatct cgaagaaact
     5761 aaaaaaacat cggatttaat ttcaagatat gcaggtattc gagtgcctag aaataaagca
     5821 attgttggcc aaaatgcatt tagtcatgaa tcaggtattc accaagatgg cgtattaaaa
     5881 catcgtgaaa catatgaaat tatgacacct caacttgttg gtgtaagcac gactgaactt
     5941 ccattaggaa aattatctgg taaacacgcc ttctcagaga agttaaaagc attaggttat
     6001 aacattgata aagaagcgca aatagattta tttaaacaat tcaagaccat tgcggacaaa
     6061 aagaaatctg tttcagatag agatattcat gcgattattc aaggttctga gcatgagcat
     6121 caagcacttt ataaattgga aacactacaa ctacaatatg tctctagcgg ccttcaaagt
     6181 gctgttgttg ttgttaaaga taaagagggt catatttacc aggattcaag tattggtact
     6241 ggttcaatcg tagcaattta caatgcagtt gatcgtattt tccagaaaga aacagaatta
     6301 attgattatc gtattaattc tgtcactgaa ggtactgatg cccaagcaga agtacatgta
     6361 aatttattga ttgaaggtaa gactgtcaat ggctttggta ttgatcatga tattttacaa
     6421 gcctcttgta aagcatacgt agaagcacat gctaaatttg cagctgaaaa tgttgagaag
     6481 gtaggtaatt aattatgact tataacattg ttgccctacc tggtgatgga atcggtccag
     6541 aaattttgaa cggatctcta tcattgcttg aaattataag taataaatat aactttaatt
     6601 atcaaataga gcaccacgaa tttggtggtg cctctattga tacattcggc gagcctttaa
     6661 ctgagaaaac cttaaatgcg tgtaaaagag cagatgctat tttacttagt gcaatcggtg
     6721 gacctaaatg gacagatcct aacaatcgac cagaacaagg attattaaaa ttgcgtaaat
     6781 ccttaaattt atttgcaaat attcgcccca ctaccgttgt caaaggcgct agttctttat
     6841 cacctttaaa ggaagaacgc gttgaaggca cagatttagt tatagtccgt gaattgacaa
     6901 gtggtattta ttttggagaa cctagacatt ttaataatca cgaggcctta gattctctta
     6961 cttatacaag agaagaaata gaacgcattg ttcacgtagc atttaaattg gccgcttcaa
     7021 gacgaggaaa actaacatca gttgataaag aaaatgtatt agcttctagt aaattgtggc
     7081 gcaaagtcgt aaatgaagta agtcaattat atccagaagt aacagtaaat cacttacttg
     7141 ttgatgcttg tagtatgcat ttaatcacaa atccaaaaca atttgacgtc atcgtatgtg
     7201 aaaacttatt tggcgatatt ttaagtgatg aagcttcagt gattcctggt tcacttggtt
     7261 tatcaccttc tgctagtttt agtaacgatg gtccaagatt gtatgagcct attcatggat
     7321 cagcaccaga tattgcaggt aaaaacgttg ccaatccatt tggaatgatt ctatctttag
     7381 cgatgtgttt acgtgaaagc ttaaatcaac cagatgctgc agatgaatta gaacaacata
     7441 tttataacat gattgaacat gggcaaacga cagcagattt aggcggcaaa ttgaatacta
     7501 ctgatatttt cgaaattcta tctcaaaaat tgaatcacta agggggagat gtaaatgggt
     7561 caaacattat ttgacaaggt gtggaacaga catgtgttat acgggaaatt gggcgaaccg
     7621 caactattat acattgattt acaccttata catgaagtta cttctcctca agcatttgaa
     7681 ggacttaggc ttcaaaacag aaaattaaga cgcccagatt taacatttgc aacactcgat
     7741 cacaatgttc ctactattga tattttcaat attaaagatg aaattgcaaa caaacaaatc
     7801 acaacattac aaaaaaacgc catagatttt ggggtgcata tttttgatat gggctctgat
     7861 gaacaaggta ttgttcacat ggtaggacct gagacaggac ttacacagcc cggcaaaaca
     7921 atcgtttgtg gtgactctca cacagcaaca catggagcat ttggtgctat tgcatttgga
     7981 attggaacaa gtgaagttga acatgttttc gcaactcaaa cgctatggca aacaaaaccc
     8041 aaaaacttaa aaatcgatat taatggtacc ttaccaacag gcgtctatgc taaggacatt
     8101 attctgcatt taattaaaac gtatggtgtt gattttggta caggctatgc tttggaattt
     8161 actggcgaaa caattaaaaa cctttcaatg gacggtcgaa tgactatttg taacatggct
     8221 atcgaaggtg gtgccaaata cggcataatc caacctgatg atataacatt tgaatatgtt
     8281 aaagggagac catttgccga taacttcgct aaatcagttg ataagtggcg tgagctatat
     8341 tctgatgacg acgcgatatt tgatcgtgta attgaacttg atgtttcaac attagaacca
     8401 caagtgacat ggggaactaa tcctgaaatg ggtgttaatt tcagtgaacc attccctgaa
     8461 atcaatgata tcaacgatca acgtgcgtat gattatatgg ggttagaacc aggtcaaaaa
     8521 gctgaagaca tcgacttagg gtatgttttt ctcggttcat gtacaaatgc tagactatca
     8581 gatttgattg aagctagtca tattgttaaa ggaaataaag ttcatccaaa tattacagct
     8641 attgtcgtac caggttctcg tacagtaaaa aaagaagcag aaaaattagg tctagatact
     8701 atctttaaaa atgcaggatt tgaatggcgt gaaccaggat gttcaatgtg tttaggcatg
     8761 aatcctgacc aagtacctga gggcgtacat tgtgcatcta caagtaatcg aaactttgaa
     8821 ggacgacaag gcaaaggtgc aagaacacat ttagtatccc ctgctatggc agcagcagca
     8881 gctattcatg gtaaatttgt ggacgtaaga aaggtggttg tttaaatggc agcaatcaaa
     8941 cctattacaa catataaagg taaaatagtc cctctcttca acgacaatat cgatacagac
     9001 caaatcattc ctaaggtaca cttaaagcgt atttcaaaaa gtggctttgg tccatttgct
     9061 tttgatgaat ggcggtactt acctgatggt tcagataatc ctgatttcaa tcctaacaaa
     9121 ccacaatata aaggggcttc tattttaatt actggagata attttggatg tggttcaagt
     9181 cgtgaacatg ctgcttgggc tcttaaggac tatggttttc atattattat tgcaggaagt
     9241 ttcagtgaca tattttatat gaattgcact aaaaatgcga tgttgcctat cgttttagaa
     9301 aaaagtgctc gtgaacatct tgcacaatat gaggaaattg aaatagattt accaaatcaa
     9361 actgtgtcat caccagacaa gcgtttccat tttgaaattg atgaaacttg gaaaaataaa
     9421 cttgtaaatg gcttagatga cattgcaatc accctacaat atgaatcatt aatagaaaaa
     9481 tatgaaaaat cactttaagg gagttgaata ttatgacagt caaaacaaca gtttctacga
     9541 aagatatcga tgaagcattt ttaagactta aagatattgt caaagaaaca cctttacaat
     9601 tagaccatta cttatctcaa aagtatgatt gtaaagttta tttaaaacga gaagatttac
     9661 aatgggtacg ttcttttaaa ttaagaggtg cttacaacgc tatttctgtt ttatcagatg
     9721 aagctaaaag taaaggtatt acatgtgcga gtgcaggtaa tcatgctcaa ggtgttgcct
     9781 atacagctaa aaaacttaat ttaaacgctg ttatctttat gccagtcact acacctttac
     9841 aaaaggtaaa tcaagtaaag ttctttggaa atagtaacgt tgaagttgta ctcactggtg
     9901 atacatttga tcactgttta gctgaagctt taacttatac aagtgaacat caaatgaact
     9961 ttatagatcc attcaataat gttcatacaa tttctggaca aggtacgctt gctaaagaaa
    10021 tgctagaaca atcaaagact gacaatgtta actttgatta tttatttgcc gctattggtg
    10081 gtggtggatt aatttcaggt attagtactt actttaaaac ctattcacct actacgaaaa
    10141 ttataggtgt tgaaccttca ggtgcaagta gtatgtatga atctgttgtc gtaaataatc
    10201 aggtagtcac attgcctaat atcgataaat ttgtggacgg tgcatctgta gctagagttg
    10261 gcgatattac atttgaaatt gcaaaagaaa atgtagatga ttacgttcaa gtagatgaag
    10321 gtgcagtttg ttctacgatt ttagacatgt attcaaaaca agcaattgta gcagaacctg
    10381 ctggcgcatt aagtgtgagt gctcttgaaa actacaaaga ccacattatg ggtaaaactg
    10441 ttgtttgtgt cattagtggt ggtaataatg atattaatcg tatgaaggaa attgaagaac
    10501 gttctttatt atatgaagaa atgaaacatt attttatttt aaacttccct caacgccctg
    10561 gtgcattaag agaatttgta aatgatgttt taggcccaca agacgatatt acaaaatttg
    10621 aatacttaaa aaaatcttct caaaatacag gtactgtcat tattggtatt caacttaaag
    10681 atcatgatga tttaatacaa ctcaaacaac gtgtaaatca tttcgatcct tccaatattt
    10741 atattaatga aaataagatg ttatattcat tgttaattta acacatagta agaaaaacag
    10801 tcataaattg atttctaatt gaaatcatct tatgactgct ttttattata ctttacattt
    10861 ctcgtttcgt cagattcaaa cgttttcact tcgccaagcc atctttcttt gtgtttgctt
    10921 tttactatgt aactttagac ataaaaaaag agaccttgcg gtctcaatgc ggctcatcgc
    10981 atccactttt tgcctggcaa cgttctactc tagcggaacg taagtttgct accttcgtcg
    11041 ctaagaacct ttcttgactt gtgacaatcg cttgcttctt tcctctcctt cggctctcgc
    11101 ttactcattt agctctacta aactcgttgc actcttttct cgtttcgtca gattcaaacg
    11161 ttttcacttc gccaagccat ctttctttgt gtttgctttt attttgacgt tttagacata
    11221 aaaaaaagag acctgcggtc tcaac
//
LOCUS       DANBWH010000081        11226 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.084, whole genome shotgun sequence.
ACCESSION   DANBWH010000081 DANBWH010000000
VERSION     DANBWH010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11226
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.084"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            201..1685
                     /locus_tag="PPY92_001443"
     CDS             201..1685
                     /locus_tag="PPY92_001443"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HDJ2625453.1"
                     /translation="MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVG
                     YTVDSHKFCQNVADQGCKLVVVNKEQSLPANVTQVVVPDTLRVASILAHTLYDYPSHQ
                     LVTFGVTGTNGKTSIATMIHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLT
                     KKIKEAVDAGAESMTLEVSSHGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHA
                     KSLLFSQLGEDLSKEKYVVLNNDDSFSEYLRTVTPYEVFSYGIDEEAQFMAKNIQESL
                     QGVSFDFVTPFGTYPVKSPYVGKFNISNIMAAMIAVWSKGTSLETIIKAVENLEPVEG
                     RLEVLDPSLPIDLIIDYAHTADGMNKLIDAVQPFVKQKLIFLVGMAGERDLTKTPEMG
                     RVACRADYVIFTPDNPANDDPKMLTAELAKGATHQNYIEFDDRAEGIKHAIDIAEPGD
                     TVVLASKGREPYQIMPGHIKVPHRDDLIGLEAAYKKFGGGPVDQ"
     gene            1666..1926
                     /locus_tag="PPY92_001444"
     CDS             1666..1926
                     /locus_tag="PPY92_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueH family protein"
                     /protein_id="HDJ2625454.1"
                     /translation="MALLINKRFIDEGKTIDVYLFEALNNQIIIAIPDWFWSYQMAMT
                     LDEETCFEAILMQLFVFKEEEEAESIASQLTDWIETYKKEKD"
     gene            1926..3488
                     /locus_tag="PPY92_001445"
     CDS             1926..3488
                     /locus_tag="PPY92_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDJ2625455.1"
                     /translation="MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVK
                     GKKTGKFATSDWMKVEQERGISVTSSVMQFDYDDYKINILDTPGHEDFSEDTYRTLMA
                     VDSAVMVIDCAKGIEPQTLKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLN
                     IETYPMNWPIGMGQSFFGIIDRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSAFE
                     QAIEELMLVEEAGEAFDNDALLSGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQ
                     TKEDVEVSPFDDSFSGFIFKIQANMDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQ
                     KITRSTSFMADDKETVNHAVAGDIIGLYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEI
                     FMKVSAKNVMKQKHFHKGIEQLVQEGAIQYYKTLHTNQIILGAVGQLQFEVFEHRMKN
                     EYNVDVVMEPVGRKIARWIENEDQITDKMNTSRSILVKDRYDDLVFLFENEFATRWFE
                     EKFLEIKLYSLL"
     gene            3790..4593
                     /locus_tag="PPY92_001446"
     CDS             3790..4593
                     /locus_tag="PPY92_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDJ2625456.1"
                     /translation="MLMDPSLILPYLWVLVVLVFLEGLLAADNAIVMAVMVKHLPPEQ
                     RKKALFYGLLGAFVFRFLALFLISIIANFWFIQAAGAVYLIYMSIKNLWQFFKHPEIE
                     SPEAGDDHHYDESGEEIKASNKSFWGTVLKIEFADIAFAIDSMLAALAIAVTLPKVGI
                     HFGGMDLGQFVVMFLGGMIGVILMRYAATWFVELLNKYPGLEGAAFAIVGWVGVKLVV
                     MVLAHPDIAVLPEHFPHGVLWQSIFWTVLIGLVIIGWLGSVVKNKKSHK"
     gene            4812..7136
                     /locus_tag="PPY92_001447"
     CDS             4812..7136
                     /locus_tag="PPY92_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDJ2625457.1"
                     /translation="MEWTLVDIGKKHVIPKSQYRRKRREFFHNEDREENLNQHQDKQN
                     IDNTTSKKADKQIHKDSIDKHERFKNSLSSHLEQRNRDVNENKAEESKSNQDSKSAYN
                     RDHYLTDDVSKKQNSLDSVDQDTEKSKYYEQNSEATLSTKSTDKVESTEMRKLSSDKN
                     KVGHEEQHVLSKPSEHDKETRIDSESSRTDSDSSMQTEKIKKDSSDGNKSSNLKSEVI
                     SDKSNTVPKLSESDDEVNNQKPLTLPEEQKLKRQQSQNEQTKTYTYGDSEQNDKSNHE
                     NDLSHHTPSISDDKDNVMRENHIVDDNPDNDINTLSLSKIDDDRKLDEKIHVEDKHKQ
                     NADSSETVGYQSQSTASHRSTEKRNTSINDHDKLNGQKTNTKTSANNNQKKATSKLNK
                     GRATNNNYSDILKKFWMMYWPKLVILMGIIILIVILNAIFNNVNKNDRMNDNNDADAQ
                     KYTTTMKNANNTVKSVVTVENETSKDSSLPKDKASQDEVGSGVVYKKSGDTLYIVTNA
                     HVVGDKENQKITFSNNKSVVGKVLGKDKWSDLAVVKATSSDSSVKEIAIGDSNNLVLG
                     EPILVVGNPLGVDFKGTVTEGIISGLNRNVPIDFDKDNKYDMLMKAFQIDASVNPGNS
                     GGAVVNREGKLIGVVAAKISMPNVENMSFAIPVNEVQKIVKDLETKGKIDYPDVGVKM
                     KNIASLNSFERQAVKLPGKVKNGVVVDQVDNNGLADQSGLKKGDVITELDGKLLEDDL
                     RFRQIIFSHKDDLKSITAKIYRDGKEKEINIKLK"
     gene            7153..8511
                     /locus_tag="PPY92_001448"
     CDS             7153..8511
                     /locus_tag="PPY92_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDJ2625458.1"
                     /translation="MSIFSQFLKRSSPQQGIVLYYIVAIVIAFLLLNLPYVHKPGVEV
                     NPIDTLFVAVSGISVTGLSPISIVDTYSTFGQLIILVILNIGGIGVMAIGTMLWVVLG
                     KHIGIRERQLIMLDNNKNTMSGTVKLIIDIVKSIFVIELVGAMLLAFYFYRDNPDLKY
                     AIMQGVFVSISATTNGGLDITGKSLIPYAHDYFVQAIVIFLIILGSIGFPVLLEVKAY
                     IQNRVTNFRFSLFTKITTSTYLFLFIVGVLAILLFEHNHAFKGLSWHQSLFYSLFQSA
                     TTRSAGLQTIDVTTLSDPTNIIMGILMFIGSSPSSVGGGIRTTTFAILILFLINFSNN
                     ADKTSIKVYNREVHIMDIQRSFAVFTMATILTFLGMLIISATENGKLTFLQVFFEVMS
                     AFGTCGLSLGVTSDISDISKVVLMVLMFIGRVGLISFIIMIAGRREPDKFHYPKERIQ
                     IG"
     gene            8650..10161
                     /locus_tag="PPY92_001449"
     CDS             8650..10161
                     /locus_tag="PPY92_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDJ2625459.1"
                     /translation="MEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQIRAQN
                     HRVILLDSGGSLAGSLAAYYYAIVAPYKRHPMIKLMNRMHYDASGVSPSEFKFGLSFL
                     TRSIALARFPWLSANIEYNVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSEM
                     EQDVSIEKTLVASKRWIRYIHEVEEPDFLIVIYHGGLNKISNSTKNKKASSNEAEKLM
                     EELGVIDLMITAHQHQTIVGQDHETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDS
                     KVIDLNEYEEDQELLDLTFYDRKAVAYWSQEIISDKGLMLSVNGLQDLVCQTHPFSQL
                     LHDAIQLAFDNDITCVHVPMNGEKGLSGQIRNEDLYHAYPYPDKPMDMTISGQNIKDI
                     LEYSYSHLDFVNEQLSLTIIDETLCTMWQGFNYEIDMNQEPGQRVMLDQIDLTKSYRV
                     TMTDYCYRNYKNYLKNAIIHESYDETMSTLIAEKLRDPNYRISCSDNFVVKNR"
     gene            complement(10341..10429)
                     /locus_tag="PPY92_001450"
     tRNA            complement(10341..10429)
                     /locus_tag="PPY92_001450"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10392..10394),aa:Ser,seq:gct)
     gene            complement(10432..10506)
                     /locus_tag="PPY92_001451"
     tRNA            complement(10432..10506)
                     /locus_tag="PPY92_001451"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10472..10474),aa:Asn,seq:gtt)
     gene            complement(10648..11217)
                     /locus_tag="PPY92_001452"
     CDS             complement(10648..11217)
                     /locus_tag="PPY92_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HDJ2625460.1"
                     /translation="MYSQNIYVIRKGDMVIRPAFDDDDQRNGSEIIRFDKTRIQNPFK
                     VQKIIERSCKFYGNTYLGKKAETNRITGISSKPPILLTPLFPTYFFPTHSDRQNENIW
                     LNMHYIESIKELKNRKCKVTFINNESIILHVSYHSLWHQYNNSIFYYYMVDKQSRMIS
                     KNPDQPIDYNKATLNVFEALTRYSLFEDK"
BASE COUNT         4013 a         1519 c         2148 g         3546 t
ORIGIN      
        1 attataccat gttataagat ggttttaata atttaagatg agccataatt gtaaaactaa
       61 ttcataatac cgtatgtttt atttttaata gtagaaatta gaaaatgctg attagtagga
      121 tataacagtg aaattataaa tttattaaca tcaacaaaac gtgtataata aacatattgt
      181 agaaaaagga gcggttcagt ttggatgcaa gtacgttgtt taagaaagta aaagtaaagc
      241 gtgtattggg ttctttagaa caacaaatag atgatatcac tactgattca cgtacagcga
      301 gagaaggtag catttttgtc gcttcagttg gatatactgt agacagtcat aagttctgtc
      361 aaaatgtagc tgatcaaggg tgtaagttgg tagtggtcaa taaagaacaa tcattaccag
      421 ctaacgtaac acaagtggtt gtgccggaca cattaagagt agctagtatt ctagcacaca
      481 cattatatga ttatccgagt catcagttag tgacatttgg tgtaacgggt acaaatggta
      541 aaacttctat tgcgacgatg attcatttaa ttcaaagaaa gttacaaaaa aatagtgcat
      601 atttaggaac taatggtttc caaattaatg aaacaaagac aaaaggtgca aatacgacac
      661 cagaaacagt ttctttaact aagaaaatta aagaagcagt tgatgcaggc gctgaatcta
      721 tgacattaga agtatcaagc catggcttag tattaggacg actgcgaggc gttgaatttg
      781 acgttgcaat attttcaaat ttaacacaag accatttaga ttttcatggc acaatggaag
      841 catacggaca cgcgaagtct ttattgttta gtcaattagg tgaagatttg tcgaaagaaa
      901 agtatgtcgt gttaaacaat gacgattcat tttctgagta tttaagaaca gtgacgcctt
      961 atgaagtatt tagttatgga attgatgagg aagcccaatt tatggctaaa aatattcaag
     1021 aatctttaca aggtgtcagc tttgattttg taacgccttt tggaacttac ccagtaaaat
     1081 cgccttatgt tggtaagttt aatatttcta atattatggc ggcaatgatt gcggtgtgga
     1141 gtaaaggtac atctttagaa acgattatta aagctgttga aaatttagaa cctgttgaag
     1201 ggcgattaga agttttagat ccttcgttac ctattgattt aattatcgat tatgcacata
     1261 cagctgatgg tatgaacaaa ttaatcgatg cagtacagcc ttttgtaaag caaaagttga
     1321 tatttttagt tggtatggca ggcgaacgtg atttaactaa aacgcctgaa atggggcgag
     1381 ttgcctgtcg tgcagattat gtcattttca caccggataa tccggcaaat gatgacccga
     1441 aaatgttaac ggcagaatta gccaaaggtg caacacatca aaactatatt gaatttgatg
     1501 atcgtgcaga agggataaaa catgcaattg acatagctga gcctggggat actgtcgttt
     1561 tagcatcaaa aggaagagaa ccatatcaaa tcatgccagg gcatattaag gtgccacatc
     1621 gagatgattt aattggcctt gaagcagctt acaaaaagtt cggtggtggc cctgttgatc
     1681 aataaaagat ttattgatga aggtaaaact attgatgttt atttattcga agcattaaat
     1741 aaccagataa tcattgctat accagattgg ttttggtcat atcagatggc aatgacatta
     1801 gatgaagaaa cttgttttga agcaatactc atgcaattgt ttgtttttaa agaagaggaa
     1861 gaggcagaat cgattgcatc acaactaaca gattggatag aaacatataa aaaggagaaa
     1921 gactaatgaa cttaaagcaa gaagttgagt ctagaaagac ttttgcgatt atttcacatc
     1981 ccgatgcagg gaaaacaacg ttaactgaaa aactattgta cttcagtggt gctattcgtg
     2041 aagcgggtac agttaaaggg aagaagactg gtaaatttgc gacaagtgac tggatgaaag
     2101 ttgaacaaga gcgtggtatt tctgtaacta gttcagtaat gcaatttgat tacgatgatt
     2161 ataaaatcaa tatcttagat acaccaggac atgaagactt ttcagaagat acgtatagaa
     2221 cattaatggc agttgacagt gctgtcatgg tcatagactg tgcaaaaggt attgaaccac
     2281 aaacattgaa gttatttaaa gtttgtaaaa tgcgtggtat tccaatcttt acattcatta
     2341 ataaattaga ccgagtaggt aaagaaccat ttgaattatt agatgaaatc gaagagacat
     2401 taaatattga aacataccct atgaattggc caattggtat gggacaaagt ttctttggca
     2461 tcattgatag aaagtctaaa acaattgaac catttagaga tgaagaaaat atattacatt
     2521 tgaatgatga ttttgagttg gaagaagatc atgcaattac aaatgatagt gcttttgaac
     2581 aagcgattga agaattaatg ttggttgaag aagcgggtga agcctttgat aatgacgcgc
     2641 tgttgagtgg agacttaaca cctgtatttt tcggttcagc tttagctaac tttggtgtac
     2701 aaaatttctt aaatgcatat gttgattttg cgccaatgcc aaatgcgaga caaacaaaag
     2761 aagacgttga agtaagcccg tttgatgatt cattttcagg atttatcttt aaaattcaag
     2821 ccaacatgga ccctaaacac cgtgatagaa ttgcctttat gcgtgtcgtt agtggtgcat
     2881 ttgaacgtgg tatggatgtt actttgcaac gtactaataa aaagcaaaag atcacacgtt
     2941 caacgtcatt tatggcagac gataaagaaa ctgtgaatca tgctgtagca ggcgatatca
     3001 ttggactata tgatactggt aattatcaaa ttggagatac tttagttggt ggaaaacaaa
     3061 cctacagttt ccaagattta ccacaattta cgccagaaat ttttatgaaa gtttctgcta
     3121 aaaacgtcat gaaacagaag catttccata aaggtattga acaattagta caagaaggtg
     3181 cgattcaata ctataaaaca ttacacacaa accaaattat tttaggtgct gttggtcagt
     3241 tacaatttga agttttcgaa catagaatga aaaacgaata taatgttgat gttgttatgg
     3301 agccagtagg ccgtaaaatt gcacgttgga ttgaaaatga agaccaaatt acagataaga
     3361 tgaacacatc aagatcgatt ttagtgaaag atagatatga cgatttagta ttcttatttg
     3421 aaaatgaatt tgcaacaaga tggtttgaag agaaattcct tgaaattaaa ttgtatagtt
     3481 tactttaaca gctcaattgt ataatcgaat ttgttacatt aaaaataatt gtttcgttga
     3541 agaaaaataa attgtatatt ttaaaagaaa aaggtatact atgatgtatc aaatgaataa
     3601 cctatggcat tttgtcagag gggagtaact taagaatcat gaccgtataa atgattcgac
     3661 actttatcgt cattacgaag atatcttccg gtaaagtggg caatttaaat tgcttagtga
     3721 gacctttgct atttatttag cataggtctt tttgtttgta cttaacttat ttatttaaag
     3781 gagttgtaca tgttaatgga tccaagtttg atcttacctt atttatgggt acttgtcgtt
     3841 ttagtatttt tagaaggctt attagcagca gataacgcga ttgttatggc tgtaatggtt
     3901 aagcacttac cacccgaaca acgtaaaaaa gctttgtttt acggtttgtt aggtgcattt
     3961 gtatttagat ttttagcatt attcttaatt agtattatcg cgaacttttg gtttattcaa
     4021 gctgcaggag cggtttactt aatttatatg tcaatcaaaa atctgtggca gttctttaaa
     4081 cacccagaaa ttgaaagtcc tgaagctgga gatgatcatc attatgatga atctggtgaa
     4141 gagattaaag caagtaacaa atcattctgg ggaactgtgt tgaaaataga atttgcagat
     4201 atcgcatttg ccattgattc tatgcttgct gctttagcta ttgctgtaac acttcctaaa
     4261 gttggtattc actttggtgg tatggactta ggtcagttcg tagtcatgtt cctaggtgga
     4321 atgattggtg ttattctaat gcgttatgca gcaacatggt ttgtagagct attaaacaaa
     4381 tatccaggac ttgaaggtgc agccttcgcg atcgttggtt gggtaggtgt taaattagtt
     4441 gtcatggtat tagcgcaccc agacatcgct gtattgcctg agcacttccc acatggcgta
     4501 ttatggcaat ctattttctg gacagtacta attggattag taattatcgg ttggttaggt
     4561 tcagttgtta aaaataaaaa atcgcataaa taattgatgt gaagcggaca atcttaattt
     4621 agtttaaggt tgtccttttt catttaattg agtgatttat gaaaaatgga ttttgaagaa
     4681 tgtgaatcaa aagatgcgat atagtattaa gaaaatgtgc cttttatatt tagcattttt
     4741 tcaatagaaa ttatatagat tttaaagcaa attaggtgtt aatgtgtcat aatgataagt
     4801 gattttattg aatggagtgg acattagtgg atattggtaa aaaacatgta attcctaaaa
     4861 gtcagtaccg acgtaagcgt cgtgaattct tccacaacga agacagagaa gaaaatttaa
     4921 atcaacatca agataaacaa aatatagata atacaacatc aaaaaaagca gataagcaaa
     4981 tacataaaga ttcaattgat aagcacgaac gttttaaaaa tagtttatca tcgcatttag
     5041 aacagagaaa ccgtgatgtt aatgagaata aagctgaaga aagtaaaagt aatcaggata
     5101 gtaagtcagc atataacaga gatcattatt taacagacga tgtatctaaa aaacaaaatt
     5161 cattagattc agtggaccaa gatacagaga aatcaaaata ttatgagcaa aattctgaag
     5221 cgactttatc aactaaatca accgataaag tagaatcaac tgaaatgaga aagctaagtt
     5281 cagataaaaa caaagttggt catgaagagc aacatgtact ttctaaacct tcagaacatg
     5341 ataaagagac tagaattgat tctgagtctt caagaactga ttcagacagc tcgatgcaga
     5401 cagagaaaat aaaaaaagac agttcagatg gaaataaaag tagtaatctg aaatctgaag
     5461 taatatcaga caaatcaaat acagtaccaa aattgtcgga atctgatgat gaagtaaata
     5521 atcagaagcc attaacttta ccggaagaac agaaattgaa aagacagcaa agtcaaaatg
     5581 agcaaacaaa aacctataca tatggtgata gcgaacaaaa tgacaagtct aatcatgaaa
     5641 atgatttaag tcatcataca ccatcgataa gtgatgataa agataacgtt atgagagaaa
     5701 atcatattgt tgacgataat cctgataatg atatcaatac actatcatta tcaaaaatag
     5761 atgacgatcg aaaacttgat gaaaaaattc atgttgaaga taaacataaa caaaatgcag
     5821 actcgtctga aacggtggga tatcaaagtc agtcaactgc atctcatcgt agcactgaaa
     5881 aaagaaatac ttctattaat gaccatgata aattaaacgg tcaaaaaaca aatacaaaga
     5941 catcggcaaa taataatcaa aaaaaggcta catcaaaatt gaacaaaggg cgcgctacga
     6001 ataataatta tagtgacatt ttgaaaaagt tttggatgat gtattggcct aaattagtta
     6061 ttctaatggg tattattatt ctaattgtta ttttgaatgc catttttaat aatgtgaaca
     6121 aaaatgatcg catgaatgat aataatgatg cagatgctca aaaatatacg acaacgatga
     6181 aaaatgccaa taacacagtt aaatcggtcg ttacagttga aaatgaaaca tcaaaagatt
     6241 catcattacc taaagataaa gcatctcaag acgaagtggg atcaggtgtt gtatataaaa
     6301 aatctggaga tacgttatat attgttacga atgcacacgt tgtcggtgat aaagaaaatc
     6361 aaaaaataac tttctcgaat aataaaagtg ttgttgggaa agtgcttggt aaagataaat
     6421 ggtcagattt agctgttgtt aaagcaactt cttcagacag ttcagtgaaa gagatagcta
     6481 ttggagattc aaataattta gtgttaggag agccaatatt agtcgtaggt aatccacttg
     6541 gtgtagactt taaaggcact gtgacagaag gtattatttc aggtctgaac agaaatgttc
     6601 ctattgattt cgataaagat aataaatatg atatgttgat gaaagctttc caaattgatg
     6661 catcagtaaa tccaggtaac tcgggtggtg ctgtcgtcaa tagagaagga aaattaatag
     6721 gtgtagttgc agctaaaatt agtatgccaa acgttgaaaa tatgtcattt gcaatacctg
     6781 ttaatgaagt acaaaagatt gtaaaagatt tagaaacaaa aggtaaaatt gactatcccg
     6841 atgtaggtgt taaaatgaag aatattgcca gtctaaatag ttttgaaaga caagcagtta
     6901 aattgccagg aaaagttaag aacggtgttg ttgtagatca agttgacaac aatggtttag
     6961 cagatcaatc tggtctgaaa aaaggtgatg taattactga attagatggc aaacttttag
     7021 aagatgattt acgctttagg cagattatat ttagtcataa agatgacttg aaatcaatta
     7081 cagcgaagat ttatagagat ggtaaagaga aagaaattaa tattaaacta aaataatgtt
     7141 gaggtgaaca acgtgtcaat ttttagccag tttttaaaaa gatcaagccc tcaacaaggt
     7201 attgtattgt actatatcgt cgcaattgtc attgcatttt tattattaaa cttaccgtat
     7261 gttcataaac caggtgtaga agtaaatcca attgacacat tatttgttgc cgtatccgga
     7321 attagtgtta ctggattgtc tccgataagt attgtcgata cctattctac atttggacaa
     7381 ttaattatcc tcgtgatatt aaatattggt ggaattggcg tcatggcaat tggtacgatg
     7441 ttatgggtgg tactaggtaa acatattgga attagagaac gtcagttaat tatgttagat
     7501 aataacaaaa acacaatgag tggtaccgtc aaattgatta ttgatattgt aaaatcaata
     7561 tttgtaatcg aactcgtagg agccatgtta ttagcatttt acttttatcg agataatcca
     7621 gatttaaaat atgcaatcat gcaaggtgtt tttgtttcta tttctgccac taccaatggt
     7681 ggattagata ttacaggtaa gtcattaatt ccttatgcac atgattattt tgtacaagcg
     7741 atagttatat ttttaataat tttaggatca atcggcttcc cagtattatt agaagttaaa
     7801 gcttatattc aaaatagggt tactaatttt agattttcat tatttactaa aattacgaca
     7861 tcaacatatt tattcctatt tattgttggg gtattagcca ttctattatt tgaacataac
     7921 catgcgttca aaggtttaag ttggcatcaa tcgttattct attcgctgtt tcaatcagcg
     7981 actacaagaa gtgcgggtct tcaaacaatt gatgtgacaa cactaagtga ccccactaat
     8041 attatcatgg gtattttaat gtttatagga tcttcgccaa gttcggttgg tggcggtatt
     8101 cgtacaacaa ctttcgctat tttaattttg tttttaatta actttagtaa taatgccgat
     8161 aaaacatcca ttaaagttta caatagagaa gtacacatta tggatattca acgttcattt
     8221 gcagtattta caatggcgac aattttaaca tttttaggaa tgctaattat atcagctact
     8281 gaaaatggta agcttacatt tttacaagta ttttttgaag tcatgtctgc atttggaact
     8341 tgtggactat cgcttggtgt cacaagtgat attagtgata tttctaaggt cgtactaatg
     8401 gtactcatgt ttataggacg tgttggctta atatcattta tcattatgat agcaggacgt
     8461 cgagaaccag ataaattcca ttatccaaaa gaacgtattc aaataggata atataatagc
     8521 aatctaagtt tagttaatat agattttaac tggaacttag attgcttttt tagtttgtat
     8581 ttttaactta ttttataaga cgattggttt tcaaaatggt aaactagtaa caatgagagg
     8641 tgtaacatga tggaaaaaaa tgaaaacatt aatgtagaga ttttaactac gtcagatatg
     8701 catagtcatt tcttaaatgg tgattatggt tcaaatattt atagagctgg tacttatgtt
     8761 aaccaaataa gagcacaaaa tcatcgcgtc attttattag atagtggcgg aagtttagct
     8821 ggctcgttag cggcctatta ttatgctatt gttgcacctt ataaacgaca tccaatgata
     8881 aagttaatga acagaatgca ttatgatgct agcggtgtga gtccaagtga attcaagttt
     8941 ggtttatcat ttttaactcg ttcaattgct ttggcacgtt ttccatggtt atcagcaaat
     9001 attgaataca atgttactaa ggagccttat ttttcaactc catattgtat taaacatttt
     9061 ggtgacttaa aaattgctat cgtaggcgtc acagcagatg gtttaatgga aaatgagtat
     9121 tctgaaatgg agcaagatgt atctattgaa aagacattag tggcatcaaa acgttggatt
     9181 agatatatcc atgaagttga agagccagat tttttgattg taatttatca tggtggattg
     9241 aataaaatta gtaatagtac gaaaaataaa aaggcaagtt cgaatgaagc tgaaaaatta
     9301 atggaagaac tcggtgttat agatttaatg attacagctc atcagcatca aacaatagta
     9361 ggtcaagatc atgaaacgta ttatgttcag gctggtcagg atgccaaaga gcttgtacat
     9421 ctttcgatta attttaaaaa gagaacaaca acttatgatg ttgaaagcat tgattctaaa
     9481 gtgattgact taaatgagta tgaagaggat caagaattat tagatttaac attctatgat
     9541 agaaaagcag tggcttattg gtcacaggaa atcataagtg ataaaggttt gatgttatca
     9601 gtgaatgggt tacaagattt agtctgtcaa acacatccat tttcgcaatt attacatgat
     9661 gcaattcagc ttgcatttga taatgatata acatgtgtcc acgtgcctat gaacggagag
     9721 aaggggttga gtggacagat tcgaaatgaa gatttgtatc atgcataccc atatccagat
     9781 aagccaatgg atatgacaat tagtggtcaa aatatcaaag atatattgga gtatagttat
     9841 tcacatttag attttgttaa cgagcaatta agcttaacaa ttattgatga aacgttatgt
     9901 acaatgtggc aaggattcaa ttatgagatt gatatgaatc aagaacctgg gcaacgagta
     9961 atgttagatc aaattgattt gactaagagt tatagagtta caatgactga ctattgttat
    10021 cgtaactaca agaattattt aaaaaatgct attatacatg aatcgtacga tgaaacaatg
    10081 agtacattaa ttgcagagaa gttaagagat ccgaattatc gtatttcatg tagtgataat
    10141 tttgtagtta aaaacaggta aatattgcta tgtaataaat taattataag ttcaacaagt
    10201 ataaaaacct ttcttagaga agattatcta ggaaaggttt ttagaatttt aaaattgata
    10261 taaaagaaca gtgaaattct cattcattat atataagtaa acaaaaaagc taaaatggta
    10321 tataccattt tagcaagtaa cggaaacgga gggattcgaa ccctcgcgcc gctttcgcga
    10381 cctacaccct tagcaggggc gcctcttcag ccaacttgag tacgtttcca atggctccac
    10441 aggtaggact cgaacctacg accgatcggt taacagccga tagctctacc actgagctac
    10501 tgtggattaa tttgtatgtg tttgaatatc aagcacaaat attattataa caagattaag
    10561 ttaatttgca atagatacct taaaaaaata tgcagaacat ttacactata tttataaaac
    10621 attccgcata ttttaaaaat aaacaattta tttatcttca aataaagaat agcgtgtcaa
    10681 tgcttcaaac acattcaatg tggctttatt ataatctatt ggttggtcgg gattttttga
    10741 tatcatgcga gattgtttat ctaccatgta atagtaaaaa atggaattgt tatattgatg
    10801 ccataaactg tggtatgaaa catgaagaat gatagattca ttattaataa atgtcacttt
    10861 acatttacga ttttttaatt ctttaatact ttcgatataa tgcatattta accaaatatt
    10921 ttcattttgt ctgtcagaat gtgttgggaa aaaataagtt ggaaataatg gtgttagtaa
    10981 aataggtggt ttactagaaa tgccagtaat gcggtttgtc tctgctttct tgccaagata
    11041 agtattacca taaaatttgc aagagcgttc aatgattttc tggactttaa aaggattttg
    11101 aatacgcgtt ttgtcaaacc gaattatttc actaccgttt ctttgatcgt catcatcaaa
    11161 tgctggtcga ataaccatgt ctcctttgcg tatcacataa atattttgag aatacataag
    11221 caaaac
//
LOCUS       DANBWH010000082        11141 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.085, whole genome shotgun sequence.
ACCESSION   DANBWH010000082 DANBWH010000000
VERSION     DANBWH010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11141
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.085"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..420
                     /locus_tag="PPY92_001453"
     CDS             <1..420
                     /locus_tag="PPY92_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HDJ2625461.1"
                     /translation="LNIDLGKMIVAQDLNLQEIFFHNLIYFFSAIFGFLTLGLLNIGM
                     LLINSGMLGFFIAHGIYSNQLKSILLVLAPHAIFEILALLIASTFSLGFLSYLYQRMV
                     KKNKIKNKMFHNFVTTIITVIVLTIIASLLEVYVTIN"
     gene            432..1154
                     /locus_tag="PPY92_001454"
     CDS             432..1154
                     /locus_tag="PPY92_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDJ2625462.1"
                     /translation="MLVLNNLQKKFTNSKMVLNNVNAKFRSGSINCIVGVNGAGKTTL
                     LNIISSILMPTKGDVYLNSESIFENSTLKKEIFYTPVNPFFYENLSAKDNLYLICSLY
                     NRKIDQITIEKTIKDVGLNIEDLNIPVYNFSSGMKQKLNFASMLLVDSNVLLLDEPFN
                     ALDHVAQKNFTRILKGLVSKEKIIIFTSHLPNTILELSENIYLLKDGCFTDKRKAQSF
                     NQTTLETWLLESQKEVIPYEEK"
     gene            1141..1329
                     /locus_tag="PPY92_001455"
     CDS             1141..1329
                     /locus_tag="PPY92_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625463.1"
                     /translation="MKKNDLLVMSVVYILTSVIMFYINGLSKQFFTYVVGFPIIYFGY
                     ICLVNLIKKEAGIIKSEK"
     gene            1319..1516
                     /locus_tag="PPY92_001456"
     CDS             1319..1516
                     /locus_tag="PPY92_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDJ2625464.1"
                     /translation="MKNNLKIARVSLSMTQKELANKVGVTRQTISLIEKGVHNPSLSL
                     CKNICSVLNKNLDEIFGEKPQ"
     gene            complement(2026..3618)
                     /locus_tag="PPY92_001457"
     CDS             complement(2026..3618)
                     /locus_tag="PPY92_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDJ2625465.1"
                     /translation="MSNLLEVNSLNVQFNYDETTVQAVKNVSFELRKKHILGIVGESG
                     SGKSITAKSILGLLPDYPDHTLTGEIIFNGQSLNNLSTSALQQIRGKDISMIFQDPLS
                     SLNPRLTIGKQITEVLFQHKRVSKSEAKSMTINILEKVGIKHATRQFDAYPHELSGGM
                     RQRVMIAMALILKPQILIADEPTTALDASTQNQLLQLMKSLYEYTETSIIFITHDLGA
                     VYQFCDDVIVMKDGSVVESGTVESIFKSPQHTYTKRLIDAIPDIHQTRPPRPLNNDIL
                     LKFDRVSVDYTSPSGSLYRAVNDINLAIRKGETLGIVGESGSGKSTLAKTVVGLKEVS
                     EGFIWYNELPLSLFKDDELKSLRQEIQMIFQDPFASINPRFKVIDVIKRPLIIHGKVK
                     DNDDIIKTVVSLLEKVGLDQSFLYRYPHELSGGQRQRVSIARALAVEPKVIVCDEAVS
                     ALDVSIQKDIIELLKQLQLDFGITYLFITHDMGVINEICDRVAVMKNGEIVELNNTED
                     IIKHPQSDYAKQLISEVAVIAK"
     gene            3575..5053
                     /locus_tag="PPY92_001458"
     CDS             3575..5053
                     /locus_tag="PPY92_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDJ2625466.1"
                     /translation="MNCTFRLLTSNKFDMMLCLPIHKCVNLFYTFVYHSNLLKIILQG
                     VIIIITYLESFKSLYHKAFYKFVLSVLSLPIFLYAVIKFFFSAKRKNFYANNSEISEI
                     ERALHQKYKYLSQQKSSTQIHKEALKIFKAQSSNTSSKNIEQAHFSTYFENLLFHKFI
                     MIKVILALPMFILLTIYLQPLVRYIFERIVMAVIVIIGVIVSVFTILYFSPLDAAYSI
                     LGQNATKAQIHQFNVLHHLNEPYFIQLWDTIKGVFTFDLGTTYKGNEVVTKAVGERIP
                     ITIIVAVLALIVALIIAIPIGIISAMKRNSWLDITLMIIALIGLSIPSFWQGLLFILA
                     FSLKLDILPPSYMPEHPISLILPVLVIGTSIAASITRMTRSSVLEVMRSDYVLTAYAK
                     GLSTTQVVIKHILKNAIIPIVTLVGLLVAELLGGSAVTEQVFNINGIGRYIVQKQLIP
                     DIPAVMGGVVYISIVISLANLIIDIFYALIDPKLRSEINERK"
     gene            5059..6222
                     /locus_tag="PPY92_001459"
     CDS             5059..6222
                     /locus_tag="PPY92_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDJ2625467.1"
                     /translation="MVKLTTKIASLKLFASYAIATYILVMLTSALNLFKGYVADTFYI
                     AETLLIILTIILIIILTTEQTWKHHDLWRRIVEVLLLLMALTGNVFTLLMFVSIRRYQ
                     RTSQIHSYNGWESFIRKTTRHRIAIIGLLILVYMLTLSIVSQFTFDTTLATKNQFNAL
                     LHGPSLAYPFGTDDFGRDLFTRVVVGTKLTFSISIISVVIAVIFGVLLGTIAGYFNHI
                     DNLIMRILDVVFAIPSLLLAVAIIASFGASIPNLIIALSIGNIPSFARTMRASVLEIK
                     RMEYVDAARITGENTWNIIWRYILPNAIAPMIVRFSLNIGVVVLTTSSLSFLGLGVAP
                     DVAEWGNILRTGSNYLETHSNLAIVPGVCIMFVVLAFNFIGDAVRDALDPRIH"
     gene            6239..8014
                     /locus_tag="PPY92_001460"
     CDS             6239..8014
                     /locus_tag="PPY92_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDJ2625468.1"
                     /translation="MKKIISIAIIVLALVLSGCGVPTKSEVAQKSSKVEVKGERPTIH
                     FLGQASYENDMNIVKDQLENAGFNVKMNIQPDYGSYRTQRQAGNYDIQIDDWMTVFGD
                     PNYAMTALFSSTGSNSLLKDKHVDQLLNKASTQNEADVKQTYKQIEDEVVFDKGYMAP
                     LYGSKKNLVYDNKVLDKNSVGLPNSRALIWQQFDYNNSRERDTRPLVMTQQDGEIPTL
                     DPIRSIAPSVYSINMNMYTRLLLLDENDHLTTKGSLSRDYAVNKDNKAFYFLLRDDDY
                     FAKVVNGQARNTGERVSAEDVKFSLDRARDKKSVPNNNTYNMHKHINDIKILKDEDID
                     QLRKEKDKDDKSIYDKLIKAYNVKSLTTDGQKVNNKDGIYQIVKITTDQSMPREVNYL
                     THSSAGILSKKFVNQVNQEYPKGYGDSSTIPANSDGKNALYASGAYIMTQKNAYQATF
                     QRNPGFNETEKGSYGSPKIKNITLKFNGDPNNALSELRNHSIDMLADVNQKHFDLIKS
                     DKNLSIIRKNGRKSVFLMLNIKKGIFKTHPNLRQAVVNAIDQDQFIKFYRGDKFKIAS
                     PITPLVDTGNEQRQDLEKVEKAINQ"
     gene            8052..10058
                     /gene="ggt"
                     /locus_tag="PPY92_001461"
     CDS             8052..10058
                     /gene="ggt"
                     /locus_tag="PPY92_001461"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDJ2625469.1"
                     /translation="MVINLNDKQTKTSKEGLISVSHPLAAKIGKDVLDQGGNAMDAVI
                     AIQLALNVVEPFASGIGGGGYLLYYEQSTGSITAFDARETAPEHVDKQFYLDDSGEYK
                     SFFDMTTHGKTVAVPAIPKLFDYIHKRYAKLSLEDLINPAIELAIEGHAANWATEKYS
                     RQQHARLTKYHETAQVFTHENQYWREGDWIVQPELGKTFQILREQGFNAFYKGDIAKQ
                     LVNVVKACGGTIILEDLANYDIQIKAPISATFKDYDIYSMGPSSSGGITVIQILKLLE
                     HVDLPSMGPRSVDYLHHLIQAMHLAYSDRAQYLADDNFHEVPVQSLIDDDYLKARSTL
                     INSNKANIDIEHGVVSDCISHTDVEENHTETTHFCVIDKEGNIASFTTSIGMIYGSGI
                     TIPGYGVLLNTTMDGFDVVDGGINEIAPYKRPLSNMAPTIVMHHGKPILTVGAPGAIS
                     IIASVAQTLINVLVFGMDIQQAIDEPRIYSSHPNRIEWEPQFSQSTILALIARGHAME
                     HKPDAYIGDVHGLQVDLNTRDASGGADDTREGTVIGGDVLSIRKQPLPSPKIYDNDTH
                     RVYFNDMQLPLYAEQVRWMHDKYWVDESVIRIIFPEVSVHIEDLRSYEIAGKNYIDIA
                     WLARKKGYQVTLKDDSLYLTDETYHSVKANTNAYYRYDRDSITR"
     gene            complement(10371..>11141)
                     /locus_tag="PPY92_001462"
     CDS             complement(10371..>11141)
                     /locus_tag="PPY92_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDJ2625470.1"
                     /translation="KAHKIFWLNLAAIIIISIVVSGGMFLAMKWEQIHLKDGLKKVLS
                     TYPIKNLETLYEIDGHDNPHYENNDQDTWYIESSYSVVGSDELLKEDRMLLKVDKNTH
                     KITGEYDTTTNDRKDATDSTYKSYPVKVVNNKIVFTKDVKDPALKQKIENNQFLIQSG
                     DLTSILNSNDLKVTHDPTTDYYNLSGKLSNDNPNVKQLKRRYNIPSNASTKVELKGMS
                     DLKGNNHQDQKLYFYFSSPGKNQIIYKESLTYNKLSEH"
BASE COUNT         3758 a         1674 c         2005 g         3704 t
ORIGIN      
        1 ttaaatattg atttaggtaa aatgatagtt gctcaagatt taaatttaca agagattttc
       61 tttcataatt taatttattt cttctctgca atatttggtt ttctgacact tggtctgtta
      121 aatataggta tgttactcat aaattctggg atgttaggct tttttatagc acatggaatc
      181 tatagcaatc aattaaaatc tattctatta gtattagcgc cacacgctat atttgagatt
      241 ttagcacttt taattgcttc aacattctca ttaggttttt tatcatacct ttatcaaaga
      301 atggtaaaga aaaataaaat aaaaaataag atgttccata attttgtaac aacaataata
      361 actgttattg tattaacaat aatcgcatca ttattagaag tttatgtaac cataaattaa
      421 ggaagtgtat catgctagtt ttaaataatc ttcaaaaaaa atttactaat tcaaaaatgg
      481 ttttgaataa tgtgaatgct aagtttagaa gtgggagcat caattgcata gtgggagtaa
      541 atggggcagg taaaacgact ttgttaaata ttattagtag tatattgatg ccaacaaaag
      601 gtgatgtata tttaaatagt gaaagtattt ttgaaaatag cacactaaaa aaagagattt
      661 tttacacacc agtaaatccc tttttttatg aaaatttaag tgctaaagat aatttgtact
      721 taatatgtag cctatataat cggaaaatag atcaaataac tattgagaaa actattaaag
      781 atgtaggttt gaatatagaa gatctaaata tacctgtata taatttttca agtggtatga
      841 agcaaaaact aaattttgct tctatgttat tagtagattc aaatgtttta ttattagatg
      901 aaccatttaa tgcccttgac catgttgctc aaaaaaattt cacacgaatt ttaaagggat
      961 tagtttctaa agaaaagata ataattttca ctagtcattt gcctaataca attttagaac
     1021 tctcagaaaa tatttatttg ttaaaagatg gatgttttac agataaacga aaagcacagt
     1081 catttaatca aaccacgtta gaaacatggt tgttagaaag tcaaaaagag gtgatacctt
     1141 atgaagaaaa atgatttgtt agtaatgagt gtagtatata ttttaacttc agtcataatg
     1201 ttctacatta atggattaag taaacaattt tttacatatg tagttggttt cccaattatt
     1261 tattttggtt atatatgtct agttaatctt ataaaaaaag aagccggaat aattaaaagt
     1321 gaaaaataat ttgaaaatag ctagagtttc actatctatg actcaaaagg agctagcaaa
     1381 taaagttgga gtaactagac aaacaatatc tcttatagaa aagggtgtac ataacccctc
     1441 tctatcacta tgtaaaaata tttgttcggt gttgaataaa aatttggatg agatatttgg
     1501 agagaaacca caataaaaga gcttattgaa agataatcta aaagtcgaca atatatttct
     1561 tctcagaaac tgctattatt ttgaaaagaa aacacacgta ttataaattt aaaataccga
     1621 aatatagcat tattaaatca aaaatagaat taaaaaagaa aattaatcga gaaaagtatt
     1681 aaatgtcatt tatttgaatg tgttaattat gaaagaaaaa gagtaattga tgttaaatat
     1741 acattgacta agctaagttg acacactcga aaatctgaat attctaataa aatataaata
     1801 atcccccgca cttcacatag tggcggggga tttattctgg cgtcaaaatg cccagagtat
     1861 gggtgtgacc cttaaatcat ggcaaggcac ttaaaacttg atatggtgcc gagccctaac
     1921 ttagcacaat atagttagta tgacaaagtc atctaacata acacatcaga ggttgcccga
     1981 tttattccag accatacagt gataaagtta cacaacgcat gacttttatt tagcaataac
     2041 tgctacttct gaaataagtt gctttgcata gtctgattgc ggatgtttga taatatcttc
     2101 tgtgttattc agttcaacga tttcgccatt tttcataact gcaacgcgat cacatatttc
     2161 attgataaca cccatgtcat gtgtgatgaa taaataagtg atgccgaagt ctaactgtaa
     2221 ttgttttaat aactcgatga tatctttttg aattgaaacg tctaaagcgg acactgcctc
     2281 gtcgcaaaca atcactttag gttctacagc aagtgctctc gcgatactta cacgctgacg
     2341 ttgcccacca gataattcgt gtggatagcg atataagaaa ctttgatcta ggccaacctt
     2401 ttctaacaac gatacgacag ttttaataat gtcatcatta tctttgactt tcccatgaat
     2461 gattagtggt cgtttaatca catcaatgac tttaaatctt ggattaatag atgcgaatgg
     2521 atcttgaaaa atcatttgta tctcttgtcg taaagatttc aattcatcat ctttaaataa
     2581 acttaatggt aattcgttat accaaataaa gccttctgac acttccttta gaccgacgac
     2641 cgtcttagct aatgtcgatt tccctgaccc tgattcaccg acaatgccta atgtttcgcc
     2701 ttttctaata gccaagttaa tatcattaac tgctcggtat aggctgccac tcggtgatgt
     2761 gtaatccacg ctcacgcgat cgaattttaa taaaatatca ttgtttaacg gtcttggcgg
     2821 acgcgtttga tgaatatcag gaatcgcatc tattaagcgt tttgtatagg tatgttgtgg
     2881 cgatttaaaa atactttcaa ccgtgccact ttcaacgaca cttccatctt tcattacaat
     2941 cacatcgtcg caaaattgat acacagcgcc taaatcgtga gtgataaaaa taatagatgt
     3001 ttctgtgtac tcataaaggg acttcattaa ctgcagtaat tgattttgtg tactggcatc
     3061 taatgccgtt gttggttcat ctgcgattaa aatttgtggc tttaaaatca atgccattgc
     3121 tatcatgaca cgttgacgca taccaccaga aagttcatgt ggataagcat caaattgtcg
     3181 agttgcatgt tttataccta ctttttctaa aatgtttatt gtcatcgact ttgcttcaga
     3241 tttagataca cgtttatgtt gaaatagtac ttctgtaatt tgtttgccaa tcgttaatct
     3301 tggattcaac gaagagagtg gatcttgaaa aatcattgaa atatccttac ctcgaatttg
     3361 ttgtaacgct gaagttgata aattatttaa cgattgccca ttaaaaataa tttctcctgt
     3421 taatgtgtga tctggataat ctggtagtag ccctaaaata gatttagcag taatactttt
     3481 tcctgatcct gattcaccaa caatacctag gatatgtttt tttcgtaatt cgaaagagac
     3541 attttttacc gcttgaactg tagtttcatc ataattgaat tgtacattca gactgttgac
     3601 ttctaataaa tttgacatga tgttgtgcct ccctattcac aaatgtgtaa atttattcta
     3661 tacttttgta tatcatagta atctactcaa gattatttta caaggagtga ttatcataat
     3721 tacttattta gaatctttta agtcgttgta tcacaaggca ttttacaagt ttgtattatc
     3781 ggtattaagc ttgccaatat ttttatatgc agtgataaag tttttctttt ctgctaagag
     3841 aaaaaatttt tacgctaata attctgaaat ttcagaaatt gaacgagcgt tacatcaaaa
     3901 atataaatat ttatcgcagc aaaagtcatc cacacaaata cataaagaag cattaaaaat
     3961 attcaaggca caaagttcta atacgagttc aaagaatatt gaacaagcac atttttcaac
     4021 atactttgaa aatttattat ttcataagtt catcatgatc aaagtaatat tggccttgcc
     4081 gatgttcatc ttattgacta tttatttaca gccattagtt agatatattt ttgagcgaat
     4141 tgtcatggct gtgattgtca tcattggtgt tattgtcagt gtgtttacca ttctgtattt
     4201 ttcaccgctt gatgcggctt atagcatact gggacaaaat gcaacaaagg cacagataca
     4261 tcaattcaat gtattacatc atcttaacga accttatttt attcaattgt gggataccat
     4321 caagggtgtt tttacctttg acttaggtac gacttacaaa gggaatgagg ttgtgactaa
     4381 agcagttggc gaaagaattc caattacaat aattgtcgca gtattagcgc taattgtggc
     4441 attaattatt gcaataccaa ttggtattat cagtgcgatg aagcgaaata gttggcttga
     4501 tatcacgtta atgataattg cattaattgg tttatctatt ccaagtttct ggcaagggct
     4561 attattcatt ttagcgttct cattgaaatt ggatattttg ccaccatctt atatgccaga
     4621 acatccaata tcgttgattt tacctgtact tgtcattgga acaagtattg ctgcttctat
     4681 cacgcgtatg acaaggtctt ctgtacttga agtaatgcgc agcgattatg ttttaactgc
     4741 ttatgcaaaa ggattatcga cgacacaagt tgttattaaa catattttga aaaatgccat
     4801 tattccaatt gtaacgttag ttggtcttct agtggcagag ttactaggcg gttcagcagt
     4861 gacggaacaa gtatttaaca ttaatggtat cggtcgttat atcgtccaaa aacaactaat
     4921 acctgatatc ccagcagtca tgggtggggt cgtatatata tcaattgtaa tatctttagc
     4981 aaacttaatt attgatatat tttatgcttt aatcgatcca aaattacgta gtgaaattaa
     5041 cgaaaggaag tgaggcatat ggtaaaactt acaacaaaga tagcttcctt aaaactattc
     5101 gcaagttatg ctatagcaac ttatatttta gttatgttaa cgagtgcatt aaatcttttt
     5161 aaaggttatg tggccgatac gttctatatt gctgaaacat tgctaatcat tttaaccatc
     5221 attttaatta tcattttaac aacggaacaa acatggaagc atcatgacct atggcgacgt
     5281 atcgtcgaag tgttgttatt gttgatggca ttaacaggca acgtatttac attattaatg
     5341 tttgtaagta ttagacgtta ccaacgtaca tcgcaaatac atagttataa cgggtgggaa
     5401 tcgtttatac gaaaaactac tagacatcgt attgcgatta tcgggttact tattttagtc
     5461 tacatgctga cattatcaat tgtgtcacaa tttacatttg atacgacatt ggctactaaa
     5521 aatcagttca atgcactgtt acatggaccg agtctagcct atccgtttgg tactgatgat
     5581 ttcggtagag acttatttac acgcgtagtt gtaggaacga agctgacatt ttcaatttca
     5641 attatttcag tagttattgc agttattttt ggtgtgttac taggcactat cgcaggttat
     5701 tttaatcata ttgataattt aataatgcga attttagatg tagtgtttgc aattccatca
     5761 ttattgttag cggtggcaat tattgcatca tttggagcaa gtattccaaa tttaattatt
     5821 gctttaagta tcggtaatat accatcattt gcacggacaa tgcgtgccag tgttttagaa
     5881 attaaacgca tggaatatgt agatgcagca cgtatcactg gtgaaaacac ttggaatatc
     5941 atatggcgtt atattttacc gaatgcgatt gcgcctatga ttgtacgttt ttcattaaat
     6001 ataggtgtgg ttgtattaac aacaagtagt ttaagtttcc taggacttgg tgttgcacct
     6061 gatgtagctg aatggggcaa cattttacgt accggtagta actacttgga aacgcacagt
     6121 aatttagcta ttgtacctgg tgtttgtatt atgttcgtcg ttttagcatt taattttata
     6181 ggtgatgcag tgcgtgatgc actagatcca agaattcatt aaaaaggtag ggatagatgt
     6241 gaagaaaatc attagtatcg caattatagt tttagcgttg gtattaagtg gttgtggtgt
     6301 ccctacgaaa tcagaagtgg ctcaaaagtc atcgaaagtt gaagtgaaag gcgagcgacc
     6361 aacaatacat ttcctaggac aagcaagtta tgaaaatgat atgaatatcg ttaaagatca
     6421 attggaaaat gcaggattta acgtgaagat gaatatccaa ccagattatg gtagctatcg
     6481 tacacaacgt caagccggca attatgatat ccaaattgat gactggatga cagtgtttgg
     6541 tgacccgaac tatgctatga cggcattatt tagttctaca ggatcaaata gtttattgaa
     6601 agataaacat gtagaccagt tgttaaataa agcttctact caaaatgaag cagatgttaa
     6661 acaaacatat aagcaaattg aagatgaagt tgtatttgat aaagggtata tggcgccttt
     6721 atatggatca aaaaagaatt tagtatatga caataaagtg ttagataaaa atagtgttgg
     6781 attgccaaat tcacgtgcat taatatggca acaatttgat tacaacaata gtagagaacg
     6841 agatacgcgg ccacttgtga tgacacaaca agatggtgaa attcctacat tggatccaat
     6901 acgttcaatt gcgccgtcag tatattcaat taatatgaat atgtacacaa ggttattatt
     6961 attagatgaa aatgatcact taacaacgaa aggttcgtta agtcgtgatt atgctgtgaa
     7021 taaggacaat aaagcatttt atttcttgtt aagagatgat gattattttg cgaaagtggt
     7081 caatggacaa gcacgtaata ctggagagcg tgtatcggct gaagatgtta agttttcttt
     7141 agatagagca cgtgataaaa agtctgtgcc taacaataat acttacaata tgcacaaaca
     7201 tataaatgac atcaagatat taaaagatga ggacatcgat cagttgcgta aagagaaaga
     7261 caaggatgat aaatcaatct atgataagtt gattaaagct tataacgtca aatcgttaac
     7321 gacagatggt caaaaagtaa ataataaaga cggtatttat caaattgtta aaattacgac
     7381 agatcaatcg atgcctcgag aggtaaatta cttaacacac tcttcggcag gcattttatc
     7441 taaaaaattt gttaatcaag taaatcaaga atatccaaaa ggatatgggg atagcagtac
     7501 aattcctgca aattcagatg gaaaaaatgc gctttatgca agtggcgcat acattatgac
     7561 acagaaaaat gcatatcaag caacgtttca acgtaatcca ggattcaacg aaacagaaaa
     7621 aggtagttat ggatcaccta aaattaaaaa tattacattg aagtttaatg gtgatccgaa
     7681 taatgcattg tcagaactta gaaatcattc aattgatatg ttggcagatg tgaatcaaaa
     7741 acattttgat ttaattaagt cggataaaaa tttaagcatt attcgcaaaa atggacgcaa
     7801 gtcagtcttt ttaatgctaa atattaaaaa aggtatattt aagacgcatc caaacttgag
     7861 acaagcagta gttaatgcga tagatcagga tcaatttatt aagttttatc gtggcgataa
     7921 atttaaaatt gcatcaccga ttacaccact tgtcgatact ggtaacgagc aacgtcaaga
     7981 tttagaaaaa gtagaaaaag ccattaatca ataatgtttt aaatatttaa cagaaagtag
     8041 gaggatatag tatggtcatt aacttaaatg acaaacagac aaaaacatct aaagaagggt
     8101 taatttccgt atcacatcct cttgcggcta aaattggtaa ggatgtatta gatcaaggtg
     8161 gcaacgccat ggatgccgtg attgcaattc aactggcatt gaatgtggta gaaccatttg
     8221 catcaggtat tggtggtggc gggtacttgc tatattatga gcaaagtact ggcagtataa
     8281 ctgcgtttga tgcacgtgag acagcacctg aacatgtaga caaacaattt tatctagatg
     8341 attcaggcga atataaatca ttttttgata tgactacaca tggtaaaact gtcgctgtgc
     8401 cagcaattcc aaagctgttt gattatattc acaagcgtta tgctaaattg tcattggaag
     8461 atttaattaa tcctgcaatt gaactagcca ttgaaggtca tgcagccaat tgggctactg
     8521 aaaaatattc gcgccagcaa cacgcacgat tgacaaagta tcatgaaacg gcacaagtat
     8581 ttacgcatga aaatcaatat tggcgtgaag gtgattggat tgtacaaccc gaattaggta
     8641 agacatttca aatattaaga gaacaagggt ttaatgcatt ttataaaggt gacattgcga
     8701 aacaattagt caatgttgtt aaagcatgtg gtgggacaat cattttagag gatctagcca
     8761 attatgacat tcagattaaa gcgccaatca gtgcgacatt taaagactat gacatttatt
     8821 caatgggacc atctagttct ggcggtatca cggtcattca aatattgaag ttattagaac
     8881 atgtcgattt accatctatg ggtccaagat ctgtcgatta cttgcatcat ttgatacaag
     8941 cgatgcattt agcatatagt gatcgcgcgc aatacttggc ggatgataat tttcatgagg
     9001 tgcctgtaca gtcattaatt gatgacgatt atttaaaagc acgcagtacg ctcattaata
     9061 gcaataaagc aaatattgat atagagcatg gtgttgtgtc tgattgcatt agtcatacag
     9121 atgttgaaga aaatcatacc gaaacaactc atttttgtgt gattgataag gaaggtaata
     9181 ttgcttcatt tacgacatca attggtatga tttatggttc aggtatcacg attccaggct
     9241 acggtgtgtt attgaatacg acaatggatg gctttgatgt agtagatggt ggtattaacg
     9301 aaattgcacc atataaacga ccactaagta acatggctcc aacgattgtg atgcatcacg
     9361 ggaagccgat attaacagta ggtgcacctg gtgccataag tatcattgct agtgttgcgc
     9421 aaacattaat caatgtatta gtgtttggca tggatattca gcaggctata gatgaaccta
     9481 gaatttatag tagccatcct aatcgcattg aatgggagcc tcaattttca caatctacaa
     9541 tattagcatt gattgcacgt ggacatgcaa tggaacataa accagatgcc tatattggag
     9601 atgtacatgg gttacaggtt gacttgaata cacgtgacgc gtcgggaggt gctgatgata
     9661 cgagggaagg cacagtgata ggtggcgatg tattatcaat tagaaaacaa ccattaccta
     9721 gtccgaaaat atacgataat gacacccatc gagtatattt caatgatatg cagttgccat
     9781 tatatgctga acaagtgcgg tggatgcatg acaaatattg ggttgatgaa agtgttatta
     9841 gaatcatttt ccctgaagtt agtgtgcata ttgaagattt aagaagttat gaaatcgccg
     9901 gaaaaaatta tatagatatt gcctggttgg cacggaagaa aggttatcaa gttaccttaa
     9961 aggatgacag tttatactta actgatgaaa catatcattc agtgaaagca aacacaaatg
    10021 catactatag atatgatcga gatagtatca caagataatt tatgtatttg atatatagtt
    10081 tgaagtggat tttgattttt gttgaacgaa agggttcgat ttcaaacttt agagtgatgc
    10141 ttgagtgtag taatgttgaa gttgttataa gaaacgctag tcgcctttgt aaaatataaa
    10201 acgccccaca aattcgtttt tgatgtacac cacaatagtt agtctaaaat ctaacttgat
    10261 gtgattcggt acaattaaat gaatgatgtg aggcgttttt ataatgtgtg attttaatat
    10321 gttaaacgat aataaatgat ttggttattt ctataattac atcataagta ttaatgttca
    10381 cttaatttat tataagtaag gctttctttg tatataattt ggttttttcc aggacttgaa
    10441 aaataaaaat aaagtttctg atcttgatga ttattgcctt ttaaatcact cattcccttt
    10501 aattccacct ttgttgatgc attactggga atattatatc tacgttttaa ttgtttaacg
    10561 tttggattat cattcgacaa cttaccagat aaattataat aatcagtggt aggatcatgt
    10621 gtgactttca aatcattgct atttaaaatg cttgttaaat caccactttg aatcaaaaat
    10681 tgattgtttt cgattttttg tttcagcgcg ggatctttta cgtcttttgt gaaaacgatt
    10741 ttattattaa ctacttttac tggataactt ttgtatgtcg agtcagtagc atcttttcta
    10801 tcgtttgtag ttgtgtcata ttcaccagtt attttatgtg tgttcttatc tacctttaac
    10861 aacatacggt cttcttttaa aagctcatct gatccaacaa ctgaataaga ggattctata
    10921 taccatgtgt cttgatcatt attttcataa tggggattat cgtgaccatc aatttcataa
    10981 agcgtttcta agtttttaat aggatacgta cttagtactt ttttaagacc atctttcaaa
    11041 tgaatttgtt cccacttcat tgccaaaaac ataccgccac tgactacaat tgaaataata
    11101 ataattgctg ctaagtttaa ccagaaaatt ttatgtgctt t
//
LOCUS       DANBWH010000083        11020 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.086, whole genome shotgun sequence.
ACCESSION   DANBWH010000083 DANBWH010000000
VERSION     DANBWH010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11020
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.086"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            312..1652
                     /locus_tag="PPY92_001463"
     CDS             312..1652
                     /locus_tag="PPY92_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDJ2625471.1"
                     /translation="MKNTFNPLQRLHFYAAIFIAPLLITLTISGIGYLFFPEVENNIY
                     KNEFFGDSDVKTHQTLNDAVHQVEQQYEGFFVSKVSILDEPYNKRITLSDMAGNQRYV
                     FLDHNNQFVADQNAKHTYSNVMRSIHSSLFTENTIINYLVELTACWTIFMILSGTYLL
                     IKKHLISNKSKALRWQKWHAMIGVIIAIPVFVLVLTGLPWSGFMGSKIAGMMDTNGDL
                     GQGELAINPPKSDLNELPWATRKNKQPASSEKGSSGHHGNAAMPQTKLDYQISIDKVV
                     VQAQKAGIKKPFSIVYPSDKNGTFIVSNTSNSGVTGLDVSPYKEQTLYFDQYSGKKLG
                     TIKYDDYGIIAKWFTWGIPLHEGHLFGILNKIINLFVCITLLVAIGMGFVSWIKRTKN
                     TAVKVPHRVKKPASISLIICLIVLGLLMPLFGLSLILVFIIELILYIKDRRAKQ"
     gene            complement(1858..2190)
                     /locus_tag="PPY92_001464"
     CDS             complement(1858..2190)
                     /locus_tag="PPY92_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HDJ2625472.1"
                     /translation="MGKKMGLGLSIALVVIGIAVVCLMIFSSQKTTYFGYMNSKTNAE
                     KVVSEKDGLVKHNIKVEPSNDFKPKKGDFVKLVSKDDGKTFYKQEIVKHDDVPHGLMM
                     KIHDMHMN"
     gene            complement(2274..3419)
                     /locus_tag="PPY92_001465"
     CDS             complement(2274..3419)
                     /locus_tag="PPY92_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDJ2625473.1"
                     /translation="MFQLLAVCPMGLEAVVAREIQELGYETNVENGRIFFEGDASAIV
                     KANLWLRTADRIKIVVGRFNATTFDELFEQTKALPWESIIDKEGNFPVQGRSVKSTLH
                     SVPDCQAITKKAIVERLRRAYNEKGWLNESGAKYPVEVAILKDNVLLTIDTSGSGLNR
                     RGYRLAQGEAPIKETLAASLIRLANWKGDTPLIDPFCGSGTIAIEACLIAQNIAPGFN
                     REFVSEQWNIMPANIYDDYRDEADKMADYDKEIEVYASDIDPEMVEIAKRNAEEVGLS
                     DIIKFSVKDVNTLTIDTEEPVALIGNPPYGERIGDREEVEEMYRYIGKLMKQHPFLST
                     YILTSNKEFEYLVNRKATKRRKLFNGYIECTYYQYWGKKTERKTIEN"
     gene            complement(3628..4007)
                     /gene="rnpB"
                     /locus_tag="PPY92_001466"
     ncRNA           complement(3628..4007)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PPY92_001466"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(4070..4414)
                     /gene="gpsB"
                     /locus_tag="PPY92_001467"
     CDS             complement(4070..4414)
                     /gene="gpsB"
                     /locus_tag="PPY92_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HDJ2625474.1"
                     /translation="MSDVSLKLSAKDIYEKDFEKTMARGYRREEVDAFLDDIIADYQK
                     MADMNNEVVKLSEENHKLKKELEELRLRVATSRPQDNKSFSSNNTTTNTSSNNVDILK
                     RISNLEKAVFGK"
     gene            complement(4428..4991)
                     /locus_tag="PPY92_001468"
     CDS             complement(4428..4991)
                     /locus_tag="PPY92_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HDJ2625475.1"
                     /translation="MVKTVYVTGYKSFELNIFKDDAPEVHYLKQFIKHKIEQLLDEGL
                     EWVLIQGQMGIELWTAEVVIELQRTYDSLKFAVITPFQGHTEKWNEHNQSKYANIIKH
                     ADYVDSIFHTSYQGPFQFKQADQFMLEHSDQTLLIYDEEQEASPKFFKQMLVDFMDKT
                     NYTCDIVTFDELTAFINDLQWSEDQSF"
     gene            complement(4984..5334)
                     /locus_tag="PPY92_001469"
     CDS             complement(4984..5334)
                     /locus_tag="PPY92_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YppE family protein"
                     /protein_id="HDJ2625476.1"
                     /translation="MNDVVESLIYEVNNMQQNFENVKSQQQDHDFYQTVKPYTEHIDS
                     MLNEIKLHREFIIEVPYMNSRKFELLIANIEQLSVECHFKRTSRKLFIEKLKSVQYDL
                     QNILDGVTKEGTYG"
     gene            complement(5389..5568)
                     /locus_tag="PPY92_001470"
     CDS             complement(5389..5568)
                     /locus_tag="PPY92_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001788819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625477.1"
                     /translation="MLLISRGVFDLNDFIRLKVFSSYNNIHDKILYLYRKFLDLLDLT
                     DNTFEKIVKSNYIEC"
     gene            5823..6500
                     /gene="recU"
                     /locus_tag="PPY92_001471"
     CDS             5823..6500
                     /gene="recU"
                     /locus_tag="PPY92_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HDJ2625478.1"
                     /translation="MLCIINNVYYQTCVVKFMNYPNGKPYRKNSAIDGGKKTAAFSNI
                     EYGGRGMSLEKDIEHSNTFYLKSDIAVIHKKPTPVQIVNVNYPKRSKAVINEAYFRTP
                     STTDYNGVYQGYYIDFEAKETKNKTSFPLNNIHDHQVEHMKNAYQQKGIVFLMIRFKT
                     LDEVYLLPYSKFEVFWKRYKDNIKKSITVDEIRKNGYHIPYQYQPRLDYLKVVDKLIL
                     DESEDRV"
     gene            6497..8680
                     /locus_tag="PPY92_001472"
     CDS             6497..8680
                     /locus_tag="PPY92_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDJ2625479.1"
                     /translation="MTENKGSSQPKKNGNNGGKSNSKKNRNVKRTIIKIIGFMIIAFF
                     VVLLLGILLFAYYAWKAPAFTEAKLQDPIPAKIYDKNGELVKTLDNGQRHEHVNLKDV
                     PKSMKDAVLATEDNRFYEHGALDYKRLFGAIGKNLTGGFGSEGASTLTQQVVKDAFLS
                     QHKSIGRKAQEAYLSYRLEQEYSKDDIFQVYLNKIYYSDGVTGIKAAAKYYFNKDLKD
                     LNLAEEAYLAGLPQVPNNYNIYDHPKAAEDRKNTVLYLMHYHKRITDKQWEDAKKIDL
                     KANLVNRTPEERQNIDTNQDSEYNSYVNFVKSELMNNKAFKDENLGNVLQSGIKIYTN
                     MDKDVQKTLQNDVDNGSFYKNKDQQVGATILDSKTGGLVAISGGRDFKDVVNRNQATD
                     PHPTGSSLKPFLAYGPAIENMKWATNHAIQDESSYQVDGSTFRNYDTKSHGTVSIYDA
                     LRQSFNIPALKAWQSVKQNAGNDAPKKFAAKLGLNYEGDIGPSEVLGGSASEFSPTQL
                     ASAFAAIANGGTYNNAHSIQKVVTRDGETIEYDHTSHKAMSDYTAYMLAEMLKGTFKP
                     YGSAYGHGVSGVNMGAKTGTGTYGAETYSQYNLPDNAAKDVWINGFTPQYTMSVWMGF
                     SKVKQYGENSFVGHSQQEYPQFLYENVMSKISSRDGEDFKRPSSVSGSIPSINVSGSQ
                     DNNTTNRSTHGGSDTSANSSGTAQSNNNTRSQQSRNSGGLTGIFN"
     gene            complement(9246..9587)
                     /locus_tag="PPY92_001473"
     CDS             complement(9246..9587)
                     /locus_tag="PPY92_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625480.1"
                     /translation="MTNITKEVFDNLEQEIDVFAKNKALGSSEAKPYLDEYHSKIIDY
                     FKQVNDITSNIDFDNLNQYPVVPMNFKERYDYMIERKYHFMGYRQMKTFKTELIKMNA
                     SYQTRLKNKQV"
     gene            complement(9592..10251)
                     /gene="nth"
                     /locus_tag="PPY92_001474"
     CDS             complement(9592..10251)
                     /gene="nth"
                     /locus_tag="PPY92_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDJ2625481.1"
                     /translation="MVSKKKALEMIDVIANMFPDAECELKHDNPFELTIAVLLSAQCT
                     DVLVNRVTTELFKKYKTPEDYLAVSDEELMNDIRSIGLYRNKAKNIKKLCQSLIDQFN
                     GEIPQTHKELESLAGVGRKTANVVMSVAFDEPSLAVDTHVERVSKRLGINRWKDNVRQ
                     VEDRLCSVIPRDRWNRSHHQLIFFGRYHCLARKPKCDICPLLEDCREGQKRYKASLKE
                     A"
     gene            complement(10241..10927)
                     /locus_tag="PPY92_001475"
     CDS             complement(10241..10927)
                     /locus_tag="PPY92_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HDJ2625482.1"
                     /translation="MDKYQLKARPVVIRRELLDHYSDLGLDEQDLVILLKLIYASETS
                     NKQPSIELLQKGSTMQPRDITMVIQNLIQRELLELQVQKDEEGRFTEYMNLDPFFEKL
                     SHILKQQSMETKEQNSKEKFKQLFRVLEDTFARPLSPYEIETLNQWIDVDKHDSAIIQ
                     AALDEANSLNKLSFKYMDRILLNWKKNNVKTIDDSRKIREKFNKPKMTHTVKTVPKFD
                     WLNGENLDGK"
BASE COUNT         3620 a         1909 c         1630 g         3861 t
ORIGIN      
        1 ttaatcatgt tactttcccc tttggttcgt tcacatagaa atgcttgctt gttaaaacga
       61 attcatacaa tatcgatgtc catttaaaca tagatcgtta aatggctcgc attgtattat
      121 gtttaaattt tatcatagct gcttgataaa acatacgaat aatcaatttt cttcaaacat
      181 tccaacttaa aagttcatat ttctaaaaat cacaaatcat cgttaaattt ttctagaaaa
      241 taaatattgt cacagtttgt tcacacacgt cacagaattg acaccgtata atagccaatt
      301 gggagggaaa tatgaaaaat acatttaatc cattacaaag attacatttc tatgcagcaa
      361 tatttattgc tccactgtta atcactttaa ccatttcggg cattggttac ttgttctttc
      421 cagaagttga aaataatatt tataagaatg agttttttgg tgacagtgat gtaaaaacgc
      481 atcaaacatt aaatgatgca gtacatcaag ttgaacaaca atatgaagga ttctttgtaa
      541 gtaaagttag catacttgat gaaccatata acaaacgaat tacactgagt gatatggcag
      601 gaaatcaacg ttacgtcttt ctagatcata acaatcaatt tgttgcagat caaaatgcga
      661 aacatacgta ttctaatgtg atgcgaagta tacatagttc tttgtttact gaaaatacta
      721 ttattaatta tttagtagag ttaaccgcat gttggacgat attcatgatt ttatctggta
      781 cttatttact cattaagaag catttaattt ctaacaaaag taaggcactt cgttggcaaa
      841 agtggcacgc aatgattgga gttatcattg caattccagt atttgtatta gttttaactg
      901 gattgccatg gtctggtttt atgggcagta aaattgccgg tatgatggac acaaacggtg
      961 accttggtca aggtgaatta gcgattaatc cacctaaatc agatttgaac gaattacctt
     1021 gggctacacg taaaaataaa cagccagctt catccgaaaa aggttcaagt ggtcatcatg
     1081 gtaatgcagc aatgcctcaa accaaattag attatcaaat atctattgat aaggtcgttg
     1141 tacaagcgca aaaagctggt attaaaaagc cgttttcaat cgtatatcca agtgataaaa
     1201 atggtacctt tattgtatct aatactagta attcaggtgt tactgggcta gatgtatcac
     1261 cttacaagga acaaacactt tatttcgacc aatatagcgg taaaaagcta ggtacgatta
     1321 aatatgatga ctacggtatt attgctaaat ggtttacatg gggcattccg cttcacgaag
     1381 gtcatttatt cggcatttta aataaaatca ttaatttatt tgtatgtatc actttattag
     1441 tagccattgg catggggttt gtctcttgga taaagcgtac aaaaaatact gcagtaaaag
     1501 taccacatcg cgtaaaaaaa ccagcatcta tatcactcat aatatgttta attgtattag
     1561 gattattaat gccattattt ggattatcac ttatccttgt atttataatt gaattaatat
     1621 tatatattaa agatcgtcgt gctaaacaat aatgcactta aagttttgaa ctgacgaaat
     1681 ttacaaaatg attctcgtct ctctaattac ttaaaacggg ttctataata aatcgtactg
     1741 atggaaagtt tttacttttt atctgtcaga ttttttgaat ttgaagataa aaaagcatct
     1801 aaaacgcata agaagataac tctacttctt caaaattacg ttatagatgc ttttttatta
     1861 attcatatgc atgtcgtgaa ttttcatcat taaaccgtgt gggacgtcat catgtttaac
     1921 aatctcttgt ttataaaatg tcttcccatc atctttagaa actaatttta caaagtctcc
     1981 ttttttcggt ttaaaatcat ttgatggttc tactttgata ttatgtttga ctaatccatc
     2041 tttttcactg acaacttttt ctgcatttgt tttactattc atataaccaa aataagtcgt
     2101 tttttgacta gaaaaaatca ttaaacatac aacggcaata ccaataacaa ccaatgctat
     2161 agataaacct agacccattt ttttacccat ttatagactc ctccattttt caaaaatacc
     2221 tattatcatg agaacttaat tattactaat tttagtcacg cattagaaac actttagttt
     2281 tcaatagttt tacgttctgt ctttttaccc cagtattgat aataagtaca ttctatatat
     2341 ccattaaata acttacgtcg cttcgttgct ttacgattta ctaaatattc aaattcttta
     2401 ttacttgtta aaatgtatgt agataaaaat ggatgttgtt tcattagttt accaatataa
     2461 cggtacattt cttcaacttc ttcacgatca ccaatacgtt caccatatgg aggatttcca
     2521 attaacgcca ccggttcttc tgtatcaatt gttaatgtat tgacatcttt tacactaaat
     2581 ttaataatat cagacaaccc aacttcttca gcgttacgct tagcaatctc taccatttct
     2641 ggatcgatat cagaagcata tacttcgatt tctttatcat aatcagccat cttatccgct
     2701 tcatcacggt aatcatcata aatatttgct ggcatgatgt tccattgctc tgatacgaac
     2761 tcgcgattaa aaccaggtgc gatattttga gcaattaaac aagcttctat agctattgta
     2821 cccgaaccgc aaaatggatc aattaaaggt gtatcacctt tccagtttgc aagacggatt
     2881 aaacttgctg ccaacgtttc tttaattggt gcttcacctt gtgctaatct ataaccacgt
     2941 ctgttcaaac cagaacctga tgtgtcgata gtcaataata cattatcttt taaaatagca
     3001 acttcaacag ggtatttggc acctgattca tttaaccaac ctttttcgtt atatgcgcga
     3061 cgtaatcgtt caacaatagc tttcttagtt atcgcctgac aatctggcac actatgtagt
     3121 gttgatttaa cgcttctacc ttgaactggg aagttacctt ctttatcaat tatagattcc
     3181 caagggagcg ctttggtttg ttcgaataat tcgtcaaacg ttgttgcgtt aaaacgtcca
     3241 acaacaattt tgattcggtc tgctgtgcgc aaccataaat ttgcctttac aattgcactt
     3301 gcgtctcctt caaaaaatat acgaccattt tcaacatttg tttcatagcc taattcttga
     3361 atttccctag caacaacagc ttctaatccc atcggacaaa ctgcaagtaa ttgaaacata
     3421 tatgattctc cttttataca ggtattttat tcttagcttg tgttttttat acatttccaa
     3481 caaatttaat cgctgataca ttaacgcatc cgcttactat tttaaaacaa ggcagtgtca
     3541 ttatatcaag acaaggcgtt aattttaagt gttcttttca tgaaaaaagc tctccatcat
     3601 ctaggagagc taaactagta gtgatatttc tataagccat gttctgttcc atcgtactca
     3661 tcacgtgcac tagtcacact ggtactcagg tgataaccat ctgtctacac cacttcattt
     3721 cgcgaagtgt gtctcgttta tacgttgaat tccgttaaac aagtgctcct accaaatttg
     3781 gattgctcac tcgaggggtt taccgcgttc caccttttat atttctataa aagctacgtc
     3841 actgtggcac tttcaaatta ctctatccat atcgaaagac ttaggatatt tcattgccgt
     3901 caaattaatg ccttgattta ttgtttcatc aagcacgaac actacaatca tctcagactg
     3961 tgtgagcatg gactttcctc tatataatat agcgattacc caaaatatca cttttaaaat
     4021 tataacatag tcattattag taagacagtt aaacttttgt atttagtaat tatttaccaa
     4081 atacagcttt ttctaagttt gaaatacgtt ttaaaatatc tacattattt gaagatgtat
     4141 ttgttgttgt attattcgaa gaaaaacttt tattgtcctg aggtcttgat gttgctacac
     4201 gtagtcttaa ttcttctaat tcttttttaa gtttatgatt ctcttctgat aattttacaa
     4261 cttcattatt catatcggcc attttttgat aatcagcaat aatgtcatct aaaaatgcat
     4321 ctacttcttc tcttctatag ccacgagcca tcgttttttc aaaatctttt tcataaatat
     4381 cttttgctga taatttcaat gaaacatctg acattttttc cacctcatta gaaactttga
     4441 tcttcagacc actgtaagtc attgatgaat gctgttaatt catcgaacgt cacaatatca
     4501 caagtatagt ttgttttatc cataaaatca actaacatct gcttgaagaa cttaggactt
     4561 gcctcttgtt cctcatcata aatgagcaat gtttgatccg aatgttcaag cataaattga
     4621 tctgcttgtt taaattgaaa aggaccttga tacgacgtat gaaaaatact atcaacataa
     4681 tctgcatgct taattatgtt ggcatactta ctttgattat gttcattcca cttttctgta
     4741 tgtccttgga acggtgtaat tacagcaaac tttaacgaat catatgttcg ttgtaattca
     4801 ataacaactt ctgcagtcca taattctata cccatttgcc cttgtattaa cacccattct
     4861 aatccttcat ccaacagttg ttcaatttta tgttttataa attgttttaa ataatgtact
     4921 tcaggtgcgt catctttaaa aatgtttaat tcgaatgatt tgtaacctgt tacataaact
     4981 gttttaacca taagtaccct cttttgttac gccatctaat atattttgta aatcatattg
     5041 gacactttta agcttttcta taaataactt tcgacttgtt cgcttaaaat gacattcgac
     5101 agaaagttgt tcaatgttag caatcagtag ctcaaatttc cttgaattca tataaggtac
     5161 ttctataata aattcacgat gtaatttgat ctcattgagc atgctatcaa tatgttcagt
     5221 atatggcttt acagtttggt aaaaatcatg atcttgttgt tgtgatttca cattttcaaa
     5281 attttgttgc atgttgttaa cttcataaat tagtgattcg actacatcat tcatgaggac
     5341 gcctcctaca ttttttaatt tatcacaata tactgtattc gtcatgtttt aacactctat
     5401 ataatttgat ttaactattt tttcaaatgt gttatctgtt aaatcaagta aatctaaaaa
     5461 cttcctatat aaatacaaaa ttttatcgtg tatgttgtta tacgatgaaa atacttttaa
     5521 tctaataaaa tcatttaaat caaatacacc tctgctgatt aacaacacat acttgtactt
     5581 gcctcaaaaa taaaaattac tgatcatgat ttgactttta taacaaaatt caaaaatatt
     5641 gtaatgagta ttcattttta taagcaaaac ttttcaatta attaaaattg tgtgtaaaat
     5701 tccaacatta acggtaaatg tattataata ttattgtgtt aatgctgaaa tcacgttaaa
     5761 ataagctttg gtgtattgtg tctttcgcat ttatactaat ttaagtttgg tgtttcacta
     5821 taatgttgtg tataataaac aacgtttact accaaacttg tgtggtgaaa tttatgaatt
     5881 atccaaatgg taaaccatat cgtaaaaata gtgctataga cggagggaaa aagaccgctg
     5941 cctttagtaa tattgagtat ggtggacgtg gtatgtcact tgaaaaagat atcgaacatt
     6001 caaatacgtt ttatcttaaa agcgacattg cagttattca caaaaagcct acgccagtac
     6061 aaatagttaa tgtcaactat cctaagcgga gtaaagctgt gattaacgaa gcttattttc
     6121 gtacaccttc aacaactgat tacaacggcg tttatcaagg ttattatatt gattttgaag
     6181 caaaggaaac taaaaacaag acgtcctttc ctttaaataa tattcatgac catcaagtcg
     6241 aacatatgaa aaatgcatat caacaaaaag gtattgtgtt tttaatgatt cgttttaaaa
     6301 cgctagatga agtttatctt ttaccctatt caaaattcga agtattttgg aagagatata
     6361 aagataatat taaaaagtct ataacagttg atgaaatacg aaaaaatggt taccatattc
     6421 cttatcagta tcaaccaaga ttagactatc taaaagtagt tgataagttg atattagatg
     6481 aaagtgagga ccgcgtatga cggaaaacaa aggatcttct cagcctaaga aaaacggtaa
     6541 taatggtggg aaatccaact caaaaaagaa tagaaatgtg aagagaacga ttattaagat
     6601 tattggcttc atgattattg catttttcgt tgttctttta ctaggtatct tattgtttgc
     6661 ttattatgct tggaaagcac ctgcttttac cgaagctaaa ttacaagatc cgattcctgc
     6721 aaagatatat gacaagaacg gagaacttgt taaaacatta gataatggcc aaagacatga
     6781 gcatgtaaat ttaaaagacg tgccgaaatc aatgaaagac gcagtacttg caactgaaga
     6841 caatcgtttc tacgaacatg gcgcacttga ttataaacgt ttattcggtg caattggtaa
     6901 gaacttgact ggtggatttg gttctgaagg tgcctcaaca ttaacacaac aagttgttaa
     6961 agatgcattt ttatcacaac ataaatctat tggacgtaaa gctcaagaag catacttatc
     7021 atatcgttta gaacaagagt atagtaaaga tgatatcttc caagtatatc taaacaaaat
     7081 ttactattct gatggcgtaa caggtattaa agctgctgct aagtattact ttaataaaga
     7141 tttaaaagat ttaaacttag cggaagaagc ttatttagcc ggtttacctc aggttccaaa
     7201 caactataat atttatgatc atccaaaagc tgctgaagat cgtaaaaaca ctgttttata
     7261 cttaatgcat tatcataaac gcattacaga taaacagtgg gaagatgcta agaaaatcga
     7321 tttaaaagcg aacttagtaa atcgtactcc tgaagaacgt caaaacattg atacaaatca
     7381 agattctgag tataattcat acgttaactt tgtgaaatct gaattaatga ataataaagc
     7441 attcaaagat gaaaatttag gtaatgtatt acaaagtggt attaaaattt atacaaacat
     7501 ggataaagat gttcaaaaaa cattacaaaa tgatgttgat aatggtagct tctacaagaa
     7561 taaagaccaa caagttggtg caacgattct tgatagtaaa actggtggtt tagttgctat
     7621 atctggtgga cgtgatttca aagacgtcgt taacagaaac caagcaacag atcctcaccc
     7681 tactggttca tctttaaaac ctttcttagc gtatggacct gccattgaaa atatgaaatg
     7741 ggcaacaaac catgcgattc aagatgaatc ttcatatcaa gttgacggtt ctacatttag
     7801 aaactatgat acgaagagtc acggtactgt atctatttat gatgctttac gacaaagttt
     7861 caatatccca gctttaaaag catggcaatc agttaagcaa aatgctggta acgatgcacc
     7921 taagaaattc gctgccaaac ttggcttaaa ctacgaaggc gatattggtc catctgaagt
     7981 acttggtggt tctgcttcag aattctcacc aacacaatta gcatcagcat ttgctgcaat
     8041 cgctaacggt ggtacttata acaacgcgca ttcaattcaa aaagtagtta ctcgtgacgg
     8101 tgaaacaatc gaatacgatc atactagcca taaagcgatg agtgattaca ctgcatacat
     8161 gttagctgag atgctaaaag gtacatttaa accatatggt tctgcatatg gccatggtgt
     8221 atctggagta aatatgggtg ctaagacagg tactggtact tacggtgctg aaacttattc
     8281 acaatataat ttacctgata atgcagcgaa agacgtgtgg attaacggct ttacacctca
     8341 atacactatg tcagtgtgga tgggcttcag taaagttaaa caatatggtg aaaactcatt
     8401 tgtgggacat agccaacaag aatatccaca gttcttatat gaaaatgtga tgtcaaaaat
     8461 ttcatctaga gatggcgaag actttaaacg tcctagctca gtaagtggta gtatcccatc
     8521 aatcaatgtt tctggtagtc aagataacaa cactacaaat cgtagtacac acggtggtag
     8581 tgacacatca gcaaacagca gtggtactgc acaatcaaat aacaatacta gatctcaaca
     8641 atctagaaac agcggtggat taacaggtat attcaactaa tccactcaac ataaaatcct
     8701 cagttatacc atatttatgg tgtagctgag gattttttta ggttcttcat cttttatggt
     8761 gggaaggtaa aacttcctgc tttttttata agtattcata cgctaatgct atattagtag
     8821 aaaccgtaaa atcagtcatt cacatacatt ttgaaacatc taaatagttt ttcatattca
     8881 catctttagc tatgtaaaat tattgaattt gaataagtgg ttgttcccat ttttaaaatt
     8941 atgattatat tttctcatag tcatcttaga cgctgtttta aattgaaatg tttatcaatt
     9001 ccctgtgtac gttaatcagt gaaatatcaa gatgtttgca acatattttc acaatcatag
     9061 atataagaaa tccacctacg attcgaaata ggtatcttaa agcatatctc aagatatcca
     9121 ttatgatgta gatggacagt ttttatatta agacgttgta atgacacgac acctaacata
     9181 aatacaaata tgtgtgcatg aattagtttt acatattttc aacattaaga aatgatcaat
     9241 cttgtctata cctgcttatt ctttaacctt gtttgataac tagcattcat tttaatcaat
     9301 tcagttttaa aggtcttcat ttgtcgatag cccataaaat gatatttacg ttcaatcata
     9361 taatcatatc tttctttaaa attcattggc acaacaggat attgatttaa attatcaaaa
     9421 tcaatattgc tagttatgtc attaacctgc ttaaaataat caataatttt actatggtat
     9481 tcatccaagt agggctttgc ttcactagaa cctaatgcct tatttttggc aaatacatca
     9541 atctcttgtt ctaaattatc aaatacctct tttgtaatat ttgtcatgtt atcacgcttc
     9601 tttcaaacta gctttataac gtttttgtcc ttctctacaa tcttctaata gtggacaaat
     9661 atcgcattta ggttttcggg ctaaacagtg gtatcttcca aagaaaatga gttgatgatg
     9721 gctcctattc catctatctc taggtatgac agaacataaa cggtcttcta cctgtctcac
     9781 attatctttc caacgattaa tacctaagcg tttagaaaca cgttctacat gcgtatcaac
     9841 agctaatgaa ggttcatcaa atgctacact catgactaca ttagcagttt tacgtcctac
     9901 acctgctaaa ctttctaatt ccttatgtgt ttgtggtatt tctccattaa attgatcaat
     9961 caaagattga caaagtttct taatattctt agctttgtta cgatacagac cgatagaacg
    10021 aatatcattc ataagttctt catcactgac tgccaaataa tcttcaggcg ttttgtattt
    10081 tttaaacagc tcagttgtta ctctatttac tagaacgtct gtacattgcg ctgacaataa
    10141 tacagcaata gttaattcga acggattatc atgttttaat tcacattctg catccggaaa
    10201 catatttgct ataacatcaa tcatttctaa tgcttttttc ttacttacca tcaaggttct
    10261 ccccgtttaa ccaatcaaat ttaggtaccg ttttaactgt gtgcgtcatt ttcggtttat
    10321 taaatttttc tcttattttt ctagaatcgt caattgtttt gacattgttt ttcttccaat
    10381 taagtaaaat acgatccata tatttaaagc taagtttatt caaactattc gcctcgtcta
    10441 atgccgcttg tataattgca ctatcgtgtt tatcaacatc aatccattga tttaacgttt
    10501 ctatttcata tggagataac ggccttgcaa atgtatcctc taaaactcta aataattgtt
    10561 taaatttttc tttactattt tgctctttcg tttccatact ttgttgcttc aatatatgac
    10621 ttaatttttc gaaaaaagga tctagattca tatattcggt aaatctacct tcttcatctt
    10681 tttgaacttg taattctagc aattcacgtt gtatcaaatt ttgaataacc attgtaatat
    10741 cacgtggttg catagttgag cccttctgaa gtaattcaat tgaaggctgt ttatttgatg
    10801 tttcggaagc ataaatcaat ttaagcaaaa tgactaaatc ttgctcatct aaacctaagt
    10861 cactgtaatg gtctaataat tctcttcgta tcactacagg tcttgctttt aattgatatt
    10921 tatccatgtt cgtgccccct tttaaattta accattactt aatagtataa atcattttct
    10981 ttaattttcg actaaaatgc ttaatatatt taactagtag
//
LOCUS       DANBWH010000084        11007 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.087, whole genome shotgun sequence.
ACCESSION   DANBWH010000084 DANBWH010000000
VERSION     DANBWH010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11007
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.087"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(451..1380)
                     /locus_tag="PPY92_001476"
     CDS             complement(451..1380)
                     /locus_tag="PPY92_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDJ2625483.1"
                     /translation="MLLFYIIGVLVGMLIPIQTSVNSRLSLYTKSPFYTSFISFSVGT
                     ICLIILNIIINPEVFTIHFYNNQSFNYTWVVGGLLGVSFLTGNLLLLPKLGATLTVIA
                     TVAGQIIMGVIIDTFGLFGATIHDFNLIKAIGVLLLIVGIVIMNQFNKNNLLLTDQKY
                     LLFWLLLGFIFGFFPPIQTTINSALASHTHSPAFASLVSFTIGSITLLILTAIFNSSL
                     KLKTSHLKFGKLKPIYFTGGILGMAFVTANIILMPHMGAALTTLIGMFGQILMGILID
                     HFGLFGSPKIAMTSRKTIGLLCILTGIILLRLF"
     gene            complement(1575..1799)
                     /locus_tag="PPY92_001477"
     CDS             complement(1575..1799)
                     /locus_tag="PPY92_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HDJ2625484.1"
                     /translation="MTKTLELSFKSSLDKPVKLQLPQLNNVVTEQVARDSMNALVDLN
                     IIKSNSGTITKVHSAQIIDKTTTVLFEDKK"
     gene            complement(1816..2019)
                     /locus_tag="PPY92_001478"
     CDS             complement(1816..2019)
                     /locus_tag="PPY92_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625485.1"
                     /translation="MSKINHITIVLSFTKVDANGKQTEFKRRFANINPDASNDQIKTF
                     SKLIERLTGETYNNIELIKSLSI"
     gene            2178..2738
                     /locus_tag="PPY92_001479"
     CDS             2178..2738
                     /locus_tag="PPY92_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDJ2625486.1"
                     /translation="MNKVEAIKFNDDIVKMYEALKIKSERDYLFFKLAIHSGLKVSEL
                     LTITVSQVKRLIEKCTLSEMCKAHFHSLIKIRLPETLSKELLQYIEDRSLSNEDVLFQ
                     SLRTNQVLSRQQAYRIIHQASIEAGIDNVGLTTLRKTFAYHAYQKGIPIPVIQKYLGH
                     QSAIETLNFIGLENECEHSIYISLQL"
     gene            2757..5159
                     /gene="mnhA2"
                     /locus_tag="PPY92_001480"
     CDS             2757..5159
                     /gene="mnhA2"
                     /locus_tag="PPY92_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit A"
                     /protein_id="HDJ2625487.1"
                     /translation="MSLVYLLIAILVIMAMILLMSKRRALAKYAGYIALVAPVISSIY
                     FLIQIPSVAKLQYLSTSIPWIKTLDINLDLRLDGLSLMFSLIISLIGIAVFFYATQYL
                     SSRKDNLPRFYFYLTLFMFSMIGIVLSDNTILMYIFWELTSVSSFLLISYWYNNGDSQ
                     FGAIQSFMITVFGGLALLVGFIMLYIMTGTNNITDILGQADHIKNHGLFIPMIFMFLL
                     GAFTKSAQFPFHIWLPRAMAAPTPVSAYLHSATMVKAGIFLLLRFTPLLGLSNMYIYI
                     VTFVGLITMLFGSITALKQWDLKGILAYSTISQLGMIMAMVGIGGGYAQHQQDAIASI
                     YVFVLFGALFHLMNHAIFKCALFMGVGILDHEAGSRDIRILSGMRQLFPKMNLVMTIA
                     ALSMAGVPFLNGFLSKEMFLDALTQTGQLSQFSLISMIAIVFVGVIASIFTFTYALYM
                     VKEVFWTKYDSKVFTKKNIHEPWLFSLPSLILMVLVPVIFFVPNIFGKGIIVPALRGV
                     SGGNHQIDPLAPHVSQWHGFNIPLLLTIIIILLGSVLAIKVDWKKVFTGKIRQISVSK
                     GYEMVYRHFEKFATKRFKRVMQDRLNQYIIMTLGIFMIIIGYGYIRIGLPKVHQLHVS
                     EFGALEIILAIVTVTIGISLIFIRQRLTMVILNGVIGFVVTLFFIAMKAPDLALTQLV
                     VETITTILFIVSFSRLPNVPRSNANKKREIIKISVSLLMALIVVSLIFITQQTDGLSS
                     ISDFYLKADKLTGGKNIVNAILGDFRALDTLFEGLVLIITGLGIYTLLNYQDRRGQDE
                     RE"
     gene            5146..5571
                     /gene="mnhB2"
                     /locus_tag="PPY92_001481"
     CDS             5146..5571
                     /gene="mnhB2"
                     /locus_tag="PPY92_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit B"
                     /protein_id="HDJ2625488.1"
                     /translation="MKENDVVLRTVTKLVVFILLTFGFYVFFAGHNNPGGGFIGGLIF
                     SSAFILMFLAFNVEEVLESLPIDFRILMIIGALVSSITAIIPMFFGKPFLSQYETTWI
                     LPILGQIHVSTITLFELGILFSVVGVIVTVMLSLSGGRS"
     gene            5568..5912
                     /gene="mnhC2"
                     /locus_tag="PPY92_001482"
     CDS             5568..5912
                     /gene="mnhC2"
                     /locus_tag="PPY92_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit C"
                     /protein_id="HDJ2625489.1"
                     /translation="MNLILLLVIGFLVFIGTYMILSINLIRIVIGISIYTHAGNLIIM
                     SMGTYGSSRSEPLITGGNQLFVDPLLQAIVLTAIVIGFGMTAFLLVLVYRTYKVTKED
                     EIEGLRGEDDAK"
     gene            5902..7398
                     /gene="mnhD2"
                     /locus_tag="PPY92_001483"
     CDS             5902..7398
                     /gene="mnhD2"
                     /locus_tag="PPY92_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit D"
                     /protein_id="HDJ2625490.1"
                     /translation="MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTII
                     SLMLLIYVQRHRPITLDFGGWSAPFGIQFLGDSLSLIMVTTASFVITLIMAYGFGRGE
                     HKANRYHLPSFILFLSVGVIGSFLTSDLFNLYVMFEIMLLASFVLITLGQSVEQLRAA
                     IIYVVLNIIGSWLFLLGIGLLYKTVGTLNFSHIAMRLNDMGDNRTVTMISLIFLVAFS
                     AKAALVLFMWLPKAYAVLNTELAALFAALMTKVGAYALIRFFTLLFDQHNDLIHPLLA
                     TMAAITMVIGAIGVIAYKDIKKIAAYQVIISIGFIILGLGTNTFAGINGAIFYLVNDI
                     VVKTLLFFIIGSLVYITGYRQYQYLNGLAKKEPLFGVAFIIMIFAIGGVPPFSGFPGK
                     VLIFQGALQNGNYIGLALMIITSLIAMYSLFRIFFYMYFGDKDGEEVNFKKIPLYRKR
                     ILSILVVVVIAIGIAAPVVLNVTSDATELNTSDQLYQKLVNPHLKGED"
     gene            7399..7881
                     /gene="mnhE2"
                     /locus_tag="PPY92_001484"
     CDS             7399..7881
                     /gene="mnhE2"
                     /locus_tag="PPY92_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit E"
                     /protein_id="HDJ2625491.1"
                     /translation="MNQIVLNIIIAFLWVLFQDEDHFKFSTFFSGYLIGLIVIYILHR
                     FFSDDFYVRKIWVAIKFLGVYLYQLITSSISTINYILFKTKDMNPGLLSYETRLTSDW
                     AITFLTILIIITPGSTVIRISQDSKKFFIHSIDVSEKEKDSLLRSIKHYEDLILEVSR
                     "
     gene            7878..8180
                     /gene="mnhF2"
                     /locus_tag="PPY92_001485"
     CDS             7878..8180
                     /gene="mnhF2"
                     /locus_tag="PPY92_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit F"
                     /protein_id="HDJ2625492.1"
                     /translation="MIQTITHIMIISSLIIFGIALIICLFRLIKGPTTADRVVTFDTT
                     SAVVMSIVGVLSVLMGTVSFLDSIMLIAIISFVSSVSISRFIGGGHVFNGNNKRNL"
     gene            8155..8592
                     /gene="mnhG2"
                     /locus_tag="PPY92_001486"
     CDS             8155..8592
                     /gene="mnhG2"
                     /locus_tag="PPY92_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit G"
                     /protein_id="HDJ2625493.1"
                     /translation="MEITKEIFSLIAAVMLLLGSFIALISAIGIVKFQDVFLRSHAAT
                     KSSTLSVLLTLIGVLIYFIVNTGFFSVRLLLSLVFINLTSPVGMHLVARAAYRNGAYM
                     YRKNDAHTHASILLSSNEQNSTEALQLRAKKREEHRKKWYQND"
     gene            8932..10974
                     /locus_tag="PPY92_001487"
     CDS             8932..10974
                     /locus_tag="PPY92_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDJ2625494.1"
                     /translation="MEIFETILIFIAVVILSSFVHTFIPKVPLAFIQIFLGMLLFITP
                     IPVQFNFDSELFMVTMIAPLLFVEGVNVSRVHLRKYIKPVMMMALGLVITTVIGVGLF
                     IHWIWPDLPIGAAFAIAAILCPTDAVAVQAITKGKVLPKGAMTILEGESLLNDAAGII
                     SFKIAVGVLVTGAFSLVDAVQLFLIASIGGAVVGLLIGMALVRFRLTLMRRGYENINM
                     FTIIQLLTPFVTYLIAELFHASGIIAAVVAGLVHGFERDRIMQVRTQLQMSYNHTWNI
                     LGYVLNGFVFSILGFLVPEVIIKIIKTEPHNLIFLIGITIVVALAVYLFRFVWVYVLY
                     PYFYLAISPFQKMMTKNDDDNPTTEKPPKRSLYALIMTLCGVHGTISLAIALTLPYFL
                     AGHHAFTYRNDLLFIASGMVIISLVVAQVLLPLLTKPAPKTVIGNMSFKVARIYILEQ
                     VIDYLNQKSTFETSFKYGNVIKEYHDKLAFLKTVEKDDENSKELERLQKIAFNVETKT
                     LESLVDEGQITNSVLENYMRYAERTQVYRQASLIRRMIVLLRGALLKRRVQTRVNSAS
                     SLSVTDNLMELNKINKLVHYNVVSRLSKETTKDNTLEIGMVCDGYLMRIENLTPSNFF
                     NSASEDTITKIKLNALREQRRILRELIDTDEVSEGTALKLREAINYDEMVIVDSMT"
BASE COUNT         3651 a         1514 c         1896 g         3946 t
ORIGIN      
        1 ttaatgataa gaaaaacaca ttaatttagt gattaaaacc aatctatagc acgttaaagt
       61 gtatatacgt tattccgatt gataacgggt aacaaataat agaaagtacg ataattgatc
      121 atgcattaaa gcacttttat ttttcaaact cgactcaatg aattataaaa agttgtgcat
      181 tttgacttat ttttaattaa ttgaataacc aactatgaaa tattagcacg aataacataa
      241 tttttgctat taatttggtg taaattatta aaaaataaca ttaatttagg tcttaagaat
      301 gagttgacta tcgaatttgc taaattaaaa tttgttgtgt caaagtatac tacccaaatc
      361 aacgcttttc taaagatttt tagaccacaa aaaaatagct tatcactttt aaaaataatt
      421 acaagttaaa atgataagct aaaagttaat ttaaaataat cttaaaagta taatgcctgt
      481 caaaatacat aatagaccaa tagtttttct ggatgtcatt gctattttag gtgaaccaaa
      541 taatccaaag tgatctatca atatgcccat tagaatctgg ccaaacatcc caataagtgt
      601 tgttaatgct gcacccatat gaggcattaa gataatgtta gctgttacaa aagccatacc
      661 aagtataccg ccagtaaaat agataggctt taatttaccg aattttaaat gacttgtttt
      721 tagttttaaa gaactattaa aaatagcggt taaaatcaat agcgttattg acccaattgt
      781 aaatgatact aatgatgcaa aggctggtga atgagtatga cttgctaaag cactattaat
      841 tgtcgtttga ataggtggaa agaaaccaaa aataaatcct aagagaagcc aaaacagtaa
      901 atacttttga tcagttagta ataaattatt cttgttaaat tgattcatta tgacgatgcc
      961 gacaatgagt aacaatactc caattgcttt aattaaatta aaatcatgaa ttgtagcgcc
     1021 aaataagcca aatgtatcaa taatgacacc cataataatt tgacccgcaa ctgttgcaat
     1081 tacagttaat gttgcaccta attttggcaa taacaataaa ttgccagtta aaaagctaac
     1141 cccaagcaaa ccaccgacta cccatgtgta gttaaatgat tgattattgt aaaagtgaat
     1201 agtaaatact tctggattga taatgatatt taaaataatt aaacaaattg ttccaactga
     1261 aaatgaaatg aatgaagtat agaaaggtga tttagtatac agtgatagtc ttgaattgac
     1321 agatgtttgg ataggaataa gcattccaac taggacacca atgatataga aaagaagcat
     1381 atgaataaag tctcactttc caaagttaat aataaaatca tatgaataca ttatcattat
     1441 agagaaaata tacaactcat ttacaataat gttgaaaaaa acaggacatt atcataacaa
     1501 aatcacccta gacaatcatg atgaaagtct agggtgttag tatttatact aaattaattg
     1561 cgctttcttt atatctattt cttatcttca aataaaacag ttgttgtttt atcaataatt
     1621 tgagcagaat gtactttggt gatagtacca ctatttgact ttataatatt taaatctact
     1681 aatgcattca tactatcacg tgcaacttgc tcggtcacta cgttgtttaa ttgtggtaat
     1741 tgcagtttta caggcttgtc tagtgaagat ttaaaactta attcaagtgt tttagtcatg
     1801 aatatgtcct cctgattaaa ttgataaaga tttgatgagt tcgatattgt tatatgtttc
     1861 tccagtaagt cgctcaataa gtttgctgaa tgttttaatt tggtcgtttg atgcatcagg
     1921 gttaatgtta gcgaatcgac gcttaaattc tgtttgtttg ccgttagcgt ctactttagt
     1981 aaatgataat acaatagtga tgtggtttat tttactcata ttttaaaacc tcctttcaca
     2041 ctatatatcg aaataaaata ataaaatggc taattttatt ttctatgttt aaaatctata
     2101 aaaaaggcaa tagatatatg taactaaaat ataaaatagc tatgattaag acacttaaag
     2161 caaggggagg ttgagtaatg aataaagtag aagcgattaa atttaatgat gatattgtta
     2221 aaatgtatga agcgctcaag ataaaatctg aacgtgacta tttattcttt aagttagcta
     2281 tacatagtgg attgaaagta tcagaattat taacaattac agtctctcaa gttaagagac
     2341 taattgaaaa gtgtacgtta tcagaaatgt gtaaagcaca ttttcattcg ttgattaaaa
     2401 ttaggttacc agaaacatta tcgaaagaac tacttcaata tatagaggac aggagtcttt
     2461 cgaatgaaga cgttcttttt caatcactac gaacaaatca agtattatct agacagcaag
     2521 catatcgaat aattcaccaa gcatcaattg aagctggtat agataatgta ggactaacga
     2581 cattgcgtaa gacatttgca tatcatgctt atcaaaaagg tatacctata ccagtcattc
     2641 aaaagtattt agggcatcaa tctgctattg aaacactaaa ttttatcggt ttagaaaatg
     2701 agtgtgaaca tagtatttat atttcattac aattatagaa acaaaggagg ctaataatga
     2761 gtttggttta tttattaatt gctatacttg tgattatggc gatgatactt ctaatgtcta
     2821 aacgtagagc attggctaaa tatgccgggt acatagcgtt ggttgcacct gtaatttcat
     2881 ctatctattt tttgattcaa ataccatcag tagctaaact gcaatatctt tctacctcta
     2941 ttccatggat taagacatta gatattaatt tagatttacg tttagatggt ttaagtttaa
     3001 tgttttctct tattatttca cttattggaa ttgcagtatt cttctatgca actcaatatt
     3061 tatcctctcg aaaagacaat ttaccaaggt tttattttta tttaacgtta tttatgttca
     3121 gtatgattgg tattgtatta tcagacaata cgatattgat gtacattttt tgggaattaa
     3181 cgagtgtatc atcattttta ttgatttcat attggtataa caatggtgac agtcaatttg
     3241 gtgcgattca atcatttatg attacagtat ttggtggatt ggcgttatta gttggtttta
     3301 ttatgctgta tatcatgaca ggaacgaata atatcactga catattagga caagcagatc
     3361 atattaagaa tcatggattg tttatcccta tgatttttat gtttttatta ggtgcattta
     3421 caaaatcagc acaatttcca tttcatattt ggctacctag agcaatggct gcacctacac
     3481 ctgtaagtgc ttatttacat tcagccacga tggtaaaagc tggtatcttt ttattacttc
     3541 gatttacacc attattaggt cttagcaata tgtacatata tatcgtcacg tttgttggtt
     3601 taataacaat gttatttggt tcaatcacag ctttaaaaca atgggattta aaaggaatcc
     3661 tagcatattc aacaatcagt caactaggaa tgattatggc aatggtcggt atcggtggtg
     3721 gatatgctca acaccaacaa gacgcaatag catctattta tgtatttgta ttatttggtg
     3781 cgctatttca tctaatgaat catgccatct ttaaatgtgc gcttttcatg ggagtaggta
     3841 ttttagatca tgaagcaggt tcaagggata tacgaatttt aagtggaatg cgtcaactat
     3901 ttcctaaaat gaatctagtc atgacgatag cggctctatc tatggctgga gtaccatttt
     3961 taaatggatt tttaagtaaa gaaatgtttt tagatgcatt aacacaaact ggacaattat
     4021 cccaatttag tttgatttca atgatagcta tcgtgtttgt tggtgttatt gctagtattt
     4081 ttacattcac atatgcactt tacatggtta aagaagtatt ttggactaaa tatgattcta
     4141 aagtgtttac taaaaaaaat atccacgaac catggttgtt tagtttaccc tctcttatat
     4201 taatggtgct agtacctgta atcttttttg tgccaaatat atttgggaag gggattatcg
     4261 ttccagcatt aagaggtgta tcaggtggta atcatcaaat tgatccattg gcaccacatg
     4321 tttcgcaatg gcatggattt aacataccgc ttcttttaac catcatcatt attttattgg
     4381 gtagtgtact agcaatcaaa gtagattgga aaaaagtgtt cacaggtaaa attagacaga
     4441 tttcagtttc aaaagggtat gagatggtat atcgacattt tgaaaagttt gctacgaagc
     4501 gatttaaacg tgttatgcaa gatcgtttaa accaatacat tattatgacc ttaggcatat
     4561 ttatgattat cattggatat ggttatattc gaattggact tcctaaagta catcagttac
     4621 atgtttctga atttggggca ttagaaatta tattagcaat cgtaactgtc acaattggta
     4681 tttctttaat ttttatacgt caacgactga caatggtcat tttaaatgga gtcatcggat
     4741 ttgttgtgac cttattcttt atagcaatga aagcccctga tttagcattg actcagctag
     4801 tagttgaaac aataacgacg atactattta ttgtcagttt ttcaagatta ccaaacgtac
     4861 caagatctaa cgctaacaaa aaaagagaaa taattaaaat ttctgtatca ctcttgatgg
     4921 cacttattgt tgtatcatta atttttatta cacaacaaac agatggttta tcatcaatat
     4981 cagactttta tttaaaagct gacaaactaa caggtggtaa aaatattgta aatgcgatac
     5041 ttggtgactt tagagcatta gatacattat ttgaaggatt agtgttaatt attactgggc
     5101 taggtattta cacattatta aattatcaag atcggagggg acaagatgaa agagaatgat
     5161 gtcgtgttaa gaacggtcac gaaacttgtt gtatttattt tattgacttt cggattctat
     5221 gtcttcttcg caggtcataa taatcctggt ggtggcttta ttggtggttt aatatttagt
     5281 tcagcgttta ttttaatgtt tctggctttt aatgttgaag aggttttaga aagtttaccg
     5341 attgatttta gaattttaat gattattgga gcattggtat catctattac tgcgataata
     5401 cctatgtttt ttggaaaacc atttttgtct caatatgaaa caacttggat acttccaatt
     5461 ttagggcaaa ttcatgtaag tacaataaca ctttttgaat taggtatttt attctcagtt
     5521 gttggtgtta ttgtcacagt gatgttgtcg cttagcggag gtcgatcatg aatttaatat
     5581 tattactagt tataggattt ttagtgttta taggaacata tatgatttta tcaatcaatt
     5641 taattcgtat tgtaatcgga atttcaatat atactcatgc tggtaatctc attattatga
     5701 gtatgggaac gtatggttct agtagatcag aaccattaat aactggtgga aaccaattgt
     5761 ttgttgatcc cttgttacaa gctattgtac taactgcaat agttataggg tttgggatga
     5821 ctgcgttttt acttgtactt gtttatagaa cttataaagt aacaaaagaa gatgaaattg
     5881 aaggcctaag gggggaagat gatgctaagt aacttattga ttttaccaat gttattacca
     5941 ttcctttgtg ccttaatcct tgtattttta aaaaataatg atcgtatttc taaatattta
     6001 tacttaggta caatgactat caccacaatt atttcattaa tgctattaat ttatgttcag
     6061 cgtcaccgtc caattacgct agactttgga ggatggtcag cgccctttgg tatacagttt
     6121 ttaggagatt ctttaagttt gattatggtt acaaccgctt cgtttgtgat tactttaatt
     6181 atggcatacg gatttgggcg tggcgaacat aaagcaaatc gttatcactt gccatcgttc
     6241 atattatttt taagtgttgg cgtgataggc tcttttctaa catcagattt atttaattta
     6301 tacgtcatgt ttgaaattat gttactagcg tcatttgtac tcattacact tggacaatct
     6361 gtagaacaat tacgtgctgc aattatttat gttgtcttga atattattgg ttcatggcta
     6421 ttcttattag gtataggttt actttataaa acagtaggta cattaaactt ttcacatatt
     6481 gcaatgcgtt tgaatgacat gggagataat cgcactgtta caatgatttc attaatcttc
     6541 ttagtcgcat ttagtgcgaa agcagcgctg gtccttttta tgtggctacc caaagcctac
     6601 gctgtgttaa atactgagct tgcagcatta tttgcagcgt taatgaccaa agtaggggcc
     6661 tatgcattaa ttcgattctt cactttacta tttgatcaac ataatgatct catacatcca
     6721 ttgctagcaa ctatggctgc tataactatg gtcatcggcg ctataggtgt cattgcttat
     6781 aaagatatta aaaagattgc agcttaccaa gtcataatct caataggatt tatcatttta
     6841 ggtttaggaa caaacacgtt tgcaggtatt aatggtgcaa tattttattt ggtaaatgac
     6901 attgttgtaa aaacattgct attctttatt attggtagtt tagtttacat tacaggctat
     6961 cgacaatatc aatatttgaa tggcttagct aaaaaagaac ctttatttgg agttgcgttt
     7021 attataatga tttttgctat tggcggcgtg cctccattta gtggctttcc ggggaaagta
     7081 cttattttcc aaggtgcatt gcaaaatggc aattatattg gactagcgtt aatgattatt
     7141 actagtctaa ttgcaatgta cagtttattt aggatatttt tttatatgta ttttggagat
     7201 aaagatgggg aggaagttaa ttttaagaaa atcccgctat atcgaaaaag aattttaagt
     7261 attttagtag ttgtggttat cgcaatcgga attgctgcac ctgttgtgtt aaatgttaca
     7321 agtgatgcaa ctgagttgaa cacgagtgat caattatatc aaaaacttgt aaatccgcat
     7381 ttgaaaggag aggactaaat gaatcaaata gttttaaata ttatcattgc attcttatgg
     7441 gtattatttc aagatgaaga tcattttaaa ttctcgactt tcttttctgg atatctaatt
     7501 ggtttaattg tcatttatat attacacagg tttttcagcg atgattttta tgttagaaaa
     7561 atatgggtag ctattaaatt tttaggtgtt tatttatatc aattaataac atctagcatt
     7621 agcacgatta attatattct ttttaaaaca aaagatatga accctggatt actttcatat
     7681 gaaacaagac taacaagtga ttgggcaata acatttttaa caattttaat tattataact
     7741 ccagggtcta cagtaatacg aatttctcaa gactctaaaa agttttttat tcatagtatc
     7801 gacgtgtcag aaaaagaaaa agatagtttg ttaagaagta ttaagcatta tgaagactta
     7861 atattggagg tgtcgcgatg atacaaacaa taacacatat tatgattatt agttcactca
     7921 ttatttttgg aattgcatta atcatctgtt tatttagatt aatcaaggga cctacaacag
     7981 cagatcgtgt cgttacattt gatacaacaa gtgctgtcgt aatgtcaatt gtgggtgtgt
     8041 taagtgtact tatgggcacc gtttctttct tagattcaat catgctcatt gccattatat
     8101 cttttgtaag ttctgtttca atatcacgct ttattggtgg ggggcatgtg tttaatggaa
     8161 ataacaaaag aaatctttag tcttattgct gctgtgatgt tgttgttagg tagttttatt
     8221 gctcttatta gtgcaatagg tatcgtgaaa ttccaagatg ttttcttaag aagtcacgct
     8281 gcgacaaaaa gttcaacttt atccgtgtta ttaactttaa tcggtgtatt aatttatttt
     8341 attgtgaata caggattttt cagtgtgcgt ttattactgt cacttgtttt tattaattta
     8401 acttcaccag tcggcatgca cttagtcgct cgcgctgctt atcgcaacgg cgcttatatg
     8461 tatcgaaaaa atgatgctca cacacatgca tcaatattat taagttcaaa tgaacaaaac
     8521 tctacagaag cattacaatt acgtgctaaa aaacgagaag agcatcgtaa gaaatggtat
     8581 caaaacgatt gaagtgttgg ctaaattgtt aagcataaca atgcttttga aaatctgttt
     8641 tcaaaattaa tcattaaaag agtactttga aattggttag tcgattttaa ggtgctcatt
     8701 ttttgtatta taagttaatg ttgttatgtg aaagcaagtt gtttatttat acaaacaatt
     8761 agtgataaaa taaaattaaa taattgcaaa tttaagtact gcaatcgtag acatatatct
     8821 atattaaagt gcttttgtgg tcgctaactc aatttatcct ctaaagtata attacaacga
     8881 gatgtgatac ttaattttca attaaacaat actttttaga gaggtgaaag tttggaaata
     8941 tttgaaacaa ttcttatatt tatagctgtt gtgatactaa gttcgtttgt ccatactttc
     9001 atacctaaag tacccctagc atttatacaa attttcttgg gcatgttact atttattacc
     9061 ccaatccctg ttcaatttaa ttttgattct gaattgttta tggtaacaat gattgcgcct
     9121 ttgttatttg tagaaggtgt taatgtttct agagtccatt taaggaaata tattaagcca
     9181 gtgatgatga tggcattagg attagtcatt actactgtga taggtgtagg tttatttatt
     9241 cattggattt ggccagattt acctattgga gcagcatttg caattgctgc cattctttgt
     9301 cctactgatg cagtagcagt gcaagcaatc actaaaggaa aggtcttgcc aaaaggagca
     9361 atgacaattc ttgaaggtga gtcattattg aatgatgctg ctggtattat ttcatttaaa
     9421 atagctgttg gagtattagt tacaggtgct ttttcacttg ttgatgctgt tcagttgttt
     9481 ttaattgcat caattggtgg cgcagtggtt ggtttactta taggtatggc attagtaagg
     9541 ttccgattaa cattgatgcg tcgaggatat gaaaacatta atatgtttac aattattcaa
     9601 ttgttaacac catttgttac gtatttaatt gctgaattgt ttcacgcatc aggaatcatt
     9661 gcagcagtag ttgcaggact tgtacatggt ttcgaacgtg acagaattat gcaagtacgt
     9721 acacaactgc aaatgagtta caatcataca tggaatatac taggttatgt tttaaatggc
     9781 tttgtttttt caatattagg atttttagta cctgaagtta ttattaaaat tatcaaaaca
     9841 gaaccgcaca atttaatctt tttaataggc atcactattg ttgttgcttt agctgtctat
     9901 ctatttagat ttgtttgggt ttatgtctta tatccttatt tttatttagc catcagtcca
     9961 ttccaaaaaa tgatgactaa aaatgatgat gataatccaa cgactgagaa accaccaaag
    10021 cgaagtttat acgctttaat tatgacgtta tgtggtgtgc atggaacaat ttctttagca
    10081 atcgcattaa cgttacctta ttttttagca gggcatcatg cttttacgta tagaaacgac
    10141 ttattattta ttgcatctgg tatggttatt attagtttgg tagttgcgca agtattattg
    10201 ccattattaa cgaaacctgc acctaaaaca gtaattggca atatgtcgtt taaagttgct
    10261 agaatttata tattagaaca agttattgat tatctaaatc aaaaatctac tttcgaaaca
    10321 agttttaaat atggtaacgt gattaaagaa tatcatgata aattagcatt tttaaaaact
    10381 gtagagaaag atgatgaaaa ctctaaagaa ttagaacgtc tacaaaaaat tgcttttaat
    10441 gtagaaacaa aaacattaga gtctttagta gatgaaggac aaataacgaa tagtgtactt
    10501 gaaaactata tgcgttatgc tgaaagaaca caggtatata gacaagcatc attaataaga
    10561 agaatgattg tattattacg aggtgcttta ttaaaacgaa gagtacaaac gagagtgaac
    10621 tccgcatctt cacttagtgt tacggataac ttaatggaat taaataaaat taataaatta
    10681 gtccattata atgtggttag tcgtttgtct aaggaaacaa caaaagataa tacacttgaa
    10741 attggaatgg tttgtgacgg ttatttaatg cgaattgaaa acttaacacc atcaaatttc
    10801 ttcaactcag caagtgaaga tacgattact aaaattaaat taaatgcatt gagagaacaa
    10861 cgtcgcattt tacgtgagtt gattgataca gatgaagtat cagaaggtac agcgttaaaa
    10921 ctaagagaag ccatcaatta tgatgaaatg gttattgtag atagtatgac gtagttccta
    10981 attatgctaa aagggattga tgaaaaa
//
LOCUS       DANBWH010000085        10978 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.088, whole genome shotgun sequence.
ACCESSION   DANBWH010000085 DANBWH010000000
VERSION     DANBWH010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10978
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.088"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            116..2749
                     /gene="clfB"
                     /locus_tag="PPY92_001488"
     CDS             116..2749
                     /gene="clfB"
                     /locus_tag="PPY92_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfB"
                     /protein_id="HDJ2625495.1"
                     /translation="MKKRIDYLSNKQNKYSIRRFTVGTTSVIVGATILFGIGNHQAQA
                     SEQSNDTTQSSKNNASADSEKNNMIETPQLNTTANDTSDISANTNSANVDSTTKPMST
                     QTSNTTTTEPASTNETPQPTAIKNQATAAKMQDQTVPQEANSQVDNKTTNDANSIATN
                     SELKNSQTLDLPQSSPQTISNAQGTSKPSVRTRAVRSLAVAEPVVNAADAKGTNVNDK
                     VTASNFKLEKTTFDPNQSGNTFMAANFTVTDKVKSGDYFTAKLPDSLTGNGDVDYSNS
                     NNTMPIADIKSTNGDVVAKATYDILTKTYTFVFTDYVNNKENINGQFSLPLFTDRAKA
                     PKSGTYDANINIADEMFNNKITYNYSSPIAGIDKPNGANISSQIIGVDTASGQNTYKQ
                     TVFVNPKQRVLGNTWVYIKGYQDKIEESSGKVSATDTKLRIFEVNDTSKLSDSYYADP
                     NDSNLKEVTDQFKNRIYYEHPNVASIKFGDITKTYVVLVEGHYDNTGKNLKTQVIQEN
                     VDPVTNRDYSIFGWNNENVVRYGGGSADGDSAVNPKDPTPGPPVDPEPSPDPEPEPTP
                     DPEPSPDPEPEPSPDPDPDSDSDSDSGSDSDSGSDSDSESDSDSDSDSDSDSDSDSES
                     DSDSESDSDSDSDSDSDSDSDSESDSDSDSDSDSDSDSDSESDSDSESDSESDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSESDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSRVTPPNNEQKAPSNPKGEVNHSNKVSKQHKTDALPETGDKSENTNATLFGAM
                     MALLGSLLLFRKRKQDHKEKA"
     gene            2865..3626
                     /locus_tag="PPY92_001489"
     CDS             2865..3626
                     /locus_tag="PPY92_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDJ2625496.1"
                     /translation="MAYISLNYHSPTIGMHQNLTVILPEDQSFFNNDITAKPLKTLML
                     LHGLSSDETTYMRYTSIERYANEHKLAVIMPNVDHSAYANMAYGHSYYDYILEVYDYV
                     HQIFPLSKKRDDNFIAGHSMGGYGTIKFALTQGDKFAKAVPLSAVFEAQNLMDLEWND
                     FSKEAIIGNLSSVKGTEHDPYYLLDKAVAEDKQIPKLLIMCGKQDFLYQDNLDFIDYL
                     SRKNVPYQFEDGPGDHDYAYWDQAIKRAITWMVND"
     gene            3871..4326
                     /locus_tag="PPY92_001490"
     CDS             3871..4326
                     /locus_tag="PPY92_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDJ2625497.1"
                     /translation="MSTNKNDYEHMLFYFAYKTFITTADEIIEKYGMSRQHHRFLFFI
                     NKLPGITIKSLLEILEISKQGSHATLQKLKEQGLIIEKVLETDRRVKKLYSTDKGDQL
                     IAELNKAQDELLQNIYQQVGSDWYDVMEALAKGRPGFDFIKHLKDEKES"
     gene            complement(4429..4539)
                     /locus_tag="PPY92_001491"
                     /pseudo
     CDS             complement(4429..4539)
                     /locus_tag="PPY92_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501411.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4609..6033)
                     /locus_tag="PPY92_001492"
     CDS             complement(4609..6033)
                     /locus_tag="PPY92_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HDJ2625498.1"
                     /translation="MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVG
                     NTKNPKNVIFLVGDGMGPSFNTAYRYYKNDSHAKEMTPTAFDSYLKGTNRTYSNDPKQ
                     NITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLERAKEKGKSTGLVSTAELTDAT
                     PAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDG
                     YDLATNSKDLSKSDKDKVLGLFADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGF
                     FLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKDTLVVATADHSTGGL
                     TIGKDKGYEWNPQPIKSMKHSGSYMTEKMAKGEDPEKVINEGYGFEFSQDDMKKVKKE
                     NKKLQKLLKKEKDEKSADVEKQTKALQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYG
                     PRSESFSGNIENTESAKIIFDIFK"
     gene            6231..6431
                     /locus_tag="PPY92_001493"
     CDS             6231..6431
                     /locus_tag="PPY92_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625499.1"
                     /translation="MILVMLSPLLIIFFIVLSILEERKRTKKKQLEKEKANTLNQNTN
                     DTESSNQEPSLQQTKEQKDNKG"
     gene            6457..7122
                     /gene="nsaR"
                     /locus_tag="PPY92_001494"
     CDS             6457..7122
                     /gene="nsaR"
                     /locus_tag="PPY92_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system response regulator NsaR"
                     /protein_id="HDJ2625500.1"
                     /translation="MKILIVEDDFVIAESLASELKKWNYGVIVVEQFDDILSIFNQHQ
                     PQLVLLDINLPTLNGFHWCQEIRKTSNVPIIFISSRIDNMDQIMAIQMGGDDFIEKPF
                     NLSLTIAKIQALLRRTYDLSVANDSLTVKGCTLILDEAKVVYQEQNIQLSLTELQILK
                     LLFQNEDKYVSRTALIEKCWESENFIDDNTLAVNMTRLRKKLNTIGVNDFIITKKNVG
                     YKV"
     gene            7133..8020
                     /gene="nsaS"
                     /locus_tag="PPY92_001495"
     CDS             7133..8020
                     /gene="nsaS"
                     /locus_tag="PPY92_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system sensor histidine kinase NsaS"
                     /protein_id="HDJ2625501.1"
                     /translation="MTFLKSITQEIAIVIVIFALFGLMFYLYHLPLEAYLLALGVILL
                     LLLIFIGIKYLSFVKTISQQQQIENLETALYQLKNEQIEYKNDVESYFLTWVHQMKTP
                     ITAAQLLLERDEPNVVNRVRQEVIQIDNYTSLALSYLKLLNETSDISVTKISINNIIR
                     PIIMKYSIQFIDQKTKIHYEPCHHEVLTDVRWTSLMIEQLINNALKYARGKDIWIEFD
                     EQSNQLHVKDNGIGISEADLPKIFDKGYSGYNGQRQSNSSGIGLFIVKQISTHTNHPV
                     SVVSKQNEGTTFTIQFPDE"
     gene            8128..8883
                     /locus_tag="PPY92_001496"
     CDS             8128..8883
                     /locus_tag="PPY92_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDJ2625502.1"
                     /translation="MLLEVKHVKKVYGKGLNATTALNQMNLSVGAGEFVAIMGESGSG
                     KSTLLNLIASFDGLTEGDIIVDGAHLNNMKNKSKALYRQQMVGFVFQDFNLLPTMTNK
                     ENIMMPLILAGAKRKDIEQRVHQLAVQLHLEGFLNKYPSEISGGQKQRIAIARALVTK
                     PTILLADEPTGALDSKTSKALMMLFQEIHQLEQTILMVTHSNIDASYAERVIFIKDGR
                     LYHEIYRGEESQLAFQQRITDSLALVNGGSVNI"
     gene            8880..10880
                     /locus_tag="PPY92_001497"
     CDS             8880..10880
                     /locus_tag="PPY92_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDJ2625503.1"
                     /translation="MKLRLLWHIVRRQFITQRLVIIPFILAVSVLFMIEYTLVSIGLN
                     SYIKQKNDFLVPFIIIANFFMALLTFIFIFYANHFMMSQRRKEFSIFMTLGMTKKSMR
                     LIVVMETILQFVIISVVSIAGGYLLGAIFFLFIQKIMGSEVATLRYYPFDSVAMFITL
                     IIIAVLMGMLLIFNLFSINFQRPITYQHRSDSSVISRWLRYVLIVIGSAALYLGYFIA
                     LQQDTTFGAFFKIWIVIGLVIIGTYAFFVGISEIIISILQQVSKVYYHPRYFFVVVGM
                     RVRLKMNAVSLATITLLCTFLIVTLTMTLTTYRDMNHTITKLITNDYDLSFSDNSKSQ
                     IERQQTIENIKRDIQGSVNAKDFKVYETTLFRASLEKNRAYYKLKQASDDIPIDFIGN
                     EGAIFSRQSVMITAFTAQDYNRYHQNKLHLDNQSIGMITNVPIFKKQSKVGLNNEVFK
                     VKQLDAHALNLMMIQDSMVLIVKDNNQLQKVKGFYAHEQKNSTISINFNVFGKTKLTT
                     SQIQKLSKKYDASINSKSEMLMVWDRLTGGLIFVGGVVSIVLVIGIFLMMYYKQVSEA
                     YANQHNYDIMKKLGLDNGRIAKITRNQMTFLFAIPITVALIHTLISSNIVYTLLNMIG
                     INNHHIFLTSYVLAVIIISFLYMAMYKITSYIYAKVIHQQRN"
BASE COUNT         3901 a         1716 c         1932 g         3429 t
ORIGIN      
        1 tatataaata ggaagaaaac aaattttacg taattttttt cgaaaagcaa ttgatataat
       61 tcttatttca ttatacaatt tagactaatc tagaaattga aatggagtaa tatttttgaa
      121 aaaaagaatt gattatttgt cgaataagca gaataagtat tcgattagac gttttacagt
      181 aggtaccaca tcagtaatag taggggcaac tatactattt gggataggca atcatcaagc
      241 acaagcttca gaacaatcga acgatacaac gcaatcttcg aaaaataatg caagtgcaga
      301 ttccgaaaaa aacaatatga tagaaacacc tcaattaaat acaacggcta atgatacatc
      361 tgatattagt gcaaacacaa acagtgcgaa tgtagatagc acaacaaaac caatgtctac
      421 acaaacgagc aataccacta caacagagcc agcttcaaca aatgaaacac ctcaaccgac
      481 ggcaattaaa aatcaagcaa ctgctgcaaa aatgcaagat caaactgttc ctcaagaagc
      541 aaattctcaa gtagataata aaacaacgaa tgatgctaat agcatagcaa caaacagtga
      601 gcttaaaaat tctcaaacat tagatttacc acaatcatca ccacaaacga tttccaatgc
      661 gcaaggaact agtaaaccaa gtgttagaac gagagctgta cgtagtttag ctgttgctga
      721 accggtagta aatgctgctg atgctaaagg tacaaatgta aatgataaag ttacggcaag
      781 taatttcaag ttagaaaaga ctacatttga ccctaatcaa agtggtaaca catttatggc
      841 ggcaaatttt acagtgacag ataaagtgaa atcaggggat tattttacag cgaagttacc
      901 agatagttta actggtaatg gagacgtgga ttattctaat tcaaataata cgatgccaat
      961 tgcagacatt aaaagtacga atggcgatgt tgtagctaaa gcaacatatg atatcttgac
     1021 taagacgtat acatttgtct ttacagatta tgtaaataat aaagaaaata ttaacggaca
     1081 attttcatta cctttattta cagaccgagc aaaggcacct aaatcaggaa catatgatgc
     1141 gaatattaat attgcggatg aaatgtttaa taataaaatt acttataact atagttcgcc
     1201 aattgcagga attgataaac caaatggcgc gaacatttct tctcaaatta ttggtgtaga
     1261 tacagcttca ggtcaaaaca catacaagca aacagtattt gttaacccta agcaacgagt
     1321 tttaggtaat acgtgggtgt atattaaagg ctaccaagat aaaatcgaag aaagtagcgg
     1381 taaagtaagt gctacagata caaaactgag aatttttgaa gtgaatgata catctaaatt
     1441 atcagatagc tactatgcag atccaaatga ctctaacctt aaagaagtaa cagaccaatt
     1501 taaaaataga atctattatg agcatccaaa tgtagctagt attaaatttg gtgatattac
     1561 taaaacatat gtagtattag tagaagggca ttacgacaat acaggtaaga acttaaaaac
     1621 tcaggttatt caagaaaatg ttgatcctgt aacaaataga gactacagta ttttcggttg
     1681 gaataatgag aatgttgtac gttatggtgg tggaagtgct gatggtgatt cagcagtaaa
     1741 tccgaaagac ccaactccag ggccgccggt tgacccagaa ccaagtccag acccagaacc
     1801 agaaccaacg ccagatccag aaccaagtcc agacccagaa ccggaaccaa gcccagaccc
     1861 ggatccggat tcggattcag acagtgactc aggctcagac agcgactcag gttcagatag
     1921 cgactcagaa tcagatagcg attcggattc agacagtgat tcagattcag acagcgactc
     1981 agaatcagat agcgattcag aatcagatag cgactcagat tcagatagcg attcagattc
     2041 agatagcgat tcagaatcag atagcgattc ggattcagac agtgattcag attcagacag
     2101 cgactcagaa tcagatagcg actcagaatc agatagtgag tcagattcag acagtgactc
     2161 ggactcagac agtgattcag actcagatag cgattcagac tcagatagcg attcagactc
     2221 agacagcgat tcagattcag acagcgactc agaatcagac agcgactcag actcagatag
     2281 cgactcagac tcagacagcg actcagattc agatagcgat tcagactcag acagcgactc
     2341 agaatcagac agcgactcag actcagatag cgattcagac tcagacagcg actcagattc
     2401 agatagcgat tcggactcag acagcgattc agattcagac agcgactcag actcggatag
     2461 cgattcagat tcagacagcg actcagactc ggatagcgac tcggattcag atagtgactc
     2521 cgattcaaga gttacaccac caaataatga acagaaagca ccatcaaatc ctaaaggtga
     2581 agtaaaccat tctaataagg tatcaaaaca acacaaaact gatgctttac cagaaacagg
     2641 agataagagc gaaaacacaa atgcaacttt atttggtgca atgatggcat tattaggatc
     2701 attactattg tttagaaaac gcaagcaaga tcataaagaa aaagcgtaaa tactttttta
     2761 ggccgaatac atttgtattc ggtttttttg ttgaaaatga ttttaaagtg aattgattaa
     2821 gcgtaaaatg ttgataaagt agaattagaa aggggtcatg acgtatggct tatatttcat
     2881 taaactatca ttcaccaaca attggtatgc atcaaaattt gacagtcatt ttaccggaag
     2941 atcaaagctt ctttaataac gatataactg ctaaaccatt aaaaacttta atgttgttac
     3001 atggattatc aagtgatgaa acgacatata tgagatatac aagcatagaa aggtatgcga
     3061 atgaacacaa attagctgtg attatgccca atgtggatca tagcgcatat gctaacatgg
     3121 catatggtca tagctattat gattatattt tggaagtata tgattatgtt catcaaatat
     3181 ttccactttc caaaaagcgt gatgacaatt ttatagcagg tcactctatg ggaggatatg
     3241 gcacaattaa atttgcatta acacaagggg ataaatttgc caaagctgta ccattatctg
     3301 ctgtgtttga agcgcaaaat ttaatggatc tagagtggaa tgatttttca aaagaggcca
     3361 taattggcaa tctttcaagt gttaaaggaa ctgaacatga tccgtattat ttgctagaca
     3421 aagctgtagc tgaagataaa caaattccaa aattgctcat tatgtgtggt aaacaagact
     3481 ttttatatca agacaactta gattttatcg attatttatc acgtaaaaat gttccttatc
     3541 aatttgaaga tggaccagga gatcatgatt atgcatattg ggaccaagcg attaagcgtg
     3601 ctataacatg gatggtgaat gattaattat ttcctggaaa atatgtggtt gcattaaata
     3661 cacagagtga gagatacaaa ctatttacgc acgactaaca tttctgagtg tttaaattaa
     3721 atttgtatta atatgattgg cgcaatttgc tgatacgcaa aaatgtttct cgtgaaactt
     3781 agatttagct tatagtttta tcatcatttg tatgacttac attataaatt ttattataat
     3841 gaggttaacg ctttgaaagg agtcatcatc atgtcgacca ataaaaacga ttatgagcat
     3901 atgttgtttt attttgcata taaaacgttt attactaccg ctgatgaaat tatagagaag
     3961 tatggtatga gccgtcagca tcatcgtttt ttgtttttta tcaataaatt acctggtatt
     4021 actattaaat cattactaga aatattagaa atttctaaac aaggatcaca tgcaacactt
     4081 caaaaattaa aagagcaagg tctcattatt gaaaaagttt tagagactga tcgacgtgtc
     4141 aaaaaattat attcgacgga taaaggcgat caactcattg ctgaattgaa caaggcgcaa
     4201 gatgaattat tgcaaaatat atatcaacaa gtcggttcgg attggtatga tgtgatggaa
     4261 gcattggcta aagggcgacc tggctttgat tttattaagc atttgaaaga tgaaaaagaa
     4321 agctagcatc agaaatgtta aaaatcttcg cattcttaaa tttaaaaaat atgtcaaaaa
     4381 gtgtataata aaaacatata atttaattga actcagtttc aacacatctt agaaaggagt
     4441 ttgaatgatg aaaaaattag cagttatttt aacattagtt ggcggtttat actacgcatt
     4501 taaaaaatac caagaacgtg ttaaccaagc acctaacatt gagtactaaa ttaaaccata
     4561 aaaaattccc gaacaccttg ttatagtgct cgggaatttt tttatgcttt acttgaatat
     4621 atcaaatatt atttttgcgc tttctgtatt ttcgatatta ccactaaatg attctgatct
     4681 aggtccgtaa gcgtaggtat taacatcctc gcctgtatgt ccatcggaag tccaccctgt
     4741 ataagattta tcatttactg gcttctgaat tgcgtgttgt aaggcttttg tttgtttttc
     4801 tacatctgca gatttttcgt ctttttcttt tttaagtaat ttttgcaact ttttattctc
     4861 ttttttaacc tttttcatat catcttgtga aaattcaaag ccataacctt cattaataac
     4921 tttttcaggg tcttcacctt tagccatttt ttctgtcata tatgatccag agtgtttcat
     4981 cgatttaatc ggttgaggat tccattcgta tcctttatct ttaccaattg ttagaccacc
     5041 agtagagtga tctgctgttg caacgacaag tgtatcttta tgtttcttag catattgaat
     5101 ggcgtcatcg aatgcttttt caaaaccttc catttcagac ataacgcctg taatatcgtt
     5161 ggaatgcgct gatttatcta tagaagcacc ttcgaccatt aaaaagaaac cttttttatt
     5221 acgctcaagc ttactaagtg cactttgttg catatcagct aatgatggtt cgtctttaga
     5281 agcatctatt gcaagtggca tatttttatc tgcaaacaaa ccaagaactt tatctttatc
     5341 agattttgat aaatccttac tgttcgtggc aaggtcgtaa ccatcttttt tgaatttttt
     5401 atctaaattg ccattacttt taccgaaata tttagcgccg ccgcctaata aaacatcaac
     5461 tttatgcttt ccgttgattt tatctttata aaattgttta gcgatttcgt ttttatcatc
     5521 tctagaagtc acgtgtgcag catatgctgc tggtgttgca tctgttaatt cagctgttga
     5581 aacaagacca gtcgacttac ctttttcttt tgcacgttca agcactgtct ttactttctg
     5641 cttgttactg tcaacaccga tggcaccatt atatgtctta tggccagaac taaaggctgt
     5701 tccgccagct gcagaatcag taatattctg ttttgggtca tttgaatatg tacgatttgt
     5761 gccttttaaa tatgaatcaa aagcagtagg ggtcatttct ttggcatgcg aatcattttt
     5821 ataataacga taagctgtgt taaatgatgg acccatgcca tcgccaacta aaaagataac
     5881 attttttgga tttttagtat taccaaccgc gaaactttca tctttagaac ttttatcgga
     5941 ttgcgcaatt gcaggtgtga cagaactgaa aactgttgac acgataataa ggttagcaac
     6001 tgcaaatttt gtggcttttt taactgataa cataatacat cctcctgagt atatgactat
     6061 gtcttcagtg taaaagagga attttgagca attatgtagt tttagttaaa aatatgtaaa
     6121 cagagtgatt tagaataaca aaaaatgaat atatatgaca atttgttata gaaagcgtta
     6181 gaatagaagc gtgtgaaaat atagaattaa atataatttg aggtggaaaa atgatactag
     6241 taatgttatc tccattatta atcatattct ttatagtgtt gtctatttta gaggagcgta
     6301 aacgtacgaa gaaaaagcaa ctcgagaaag aaaaagcaaa tacactaaat caaaatacaa
     6361 atgacacgga aagttcaaat caagagccgt cattgcagca gactaaagaa caaaaagata
     6421 acaaaggata attcaattga aggaagaaga ttatagatga aaatattaat tgttgaagat
     6481 gattttgtta tagcagaaag tttagcatct gaacttaaaa aatggaatta cggtgttatt
     6541 gtcgttgaac aatttgatga tatactgtct atctttaacc aacatcaacc tcagcttgta
     6601 ttgctagata ttaatttgcc aacgttaaat ggttttcatt ggtgtcaaga aatccgaaaa
     6661 acatctaatg tgccaattat atttattagt tcccgtattg ataatatgga ccaaattatg
     6721 gcaatacaaa tggggggaga tgattttatc gaaaagccat ttaacttgtc attaacgatt
     6781 gccaaaattc aagcattatt gagacgaact tatgacttgt cagtagctaa tgattcattg
     6841 acggttaaag gttgtacatt aatattagat gaagcaaaag tcgtgtatca agaacaaaac
     6901 atacagctat ctttgactga attacaaata ttaaagttat tatttcaaaa tgaagataaa
     6961 tatgtaagta gaactgcttt aattgaaaaa tgttgggaat cagaaaactt catagatgat
     7021 aacacattag ctgttaacat gacgcgcctg cgtaaaaaat taaatactat tggcgttaat
     7081 gattttatca ttacaaagaa aaatgtcgga tataaagtat agggtgaatg caatgacctt
     7141 tcttaaaagt attactcagg aaatagcaat agtcatagtt atttttgctt tatttggctt
     7201 aatgttttac ctgtatcatt tgccattaga agcatattta ctagcacttg gcgttatttt
     7261 attattatta ctcatattca taggtattaa atatttaagt tttgtaaaaa ctataagcca
     7321 acaacaacaa attgaaaact tagaaactgc gttgtatcag cttaaaaatg aacaaattga
     7381 atataaaaat gatgtagaga gctacttttt aacatgggta catcaaatga aaacacccat
     7441 tactgcagca caactgttac ttgaaagaga tgagcctaat gttgttaatc gtgttcgtca
     7501 agaggttatt caaattgata actatacaag tttagcactt agttatttaa agttattaaa
     7561 tgaaacttct gatatttctg tcactaaaat ttcgattaat aatatcattc gcccaattat
     7621 tatgaaatat tcaatacagt ttattgatca aaaaacaaaa atccattatg aaccttgtca
     7681 tcacgaagta ttaactgacg ttagatggac ctctttaatg atagaacaat taataaataa
     7741 tgcacttaag tatgcgagag gtaaagatat atggattgaa tttgatgagc aatccaatca
     7801 attacacgta aaagataatg gtatcggtat tagtgaagcg gacttgccta aaatatttga
     7861 taagggctat tcaggttata atggccagcg ccaaagtaac tcaagtggga ttggtttatt
     7921 tatcgtaaaa caaatttcaa cacacacaaa ccatcctgtt tcagtcgtat ctaaacaaaa
     7981 tgagggtaca acatttacga ttcaatttcc agatgaataa aaactttcaa tattgtaagt
     8041 atactagtaa cattttttta ctaatttaaa tgttattagt atttttttgt tttaatatag
     8101 aactaacaaa gaaatgaggt gcatgccatg ttgctagaag tgaaacatgt aaaaaaggtt
     8161 tatggtaaag gtttgaatgc tacgacagca cttaatcaaa tgaatttatc agttggagct
     8221 ggtgaatttg ttgcaattat gggtgagtct gggtcaggga agtctacact actaaattta
     8281 attgcttctt ttgatggact aactgaaggt gacattattg tggatggcgc acatttaaat
     8341 aatatgaaaa ataaaagtaa agcattgtat cgtcaacaaa tggtaggttt tgtttttcaa
     8401 gattttaatc ttttaccaac aatgacgaat aaagaaaata taatgatgcc attaatttta
     8461 gctggtgcta aacgaaaaga tatagaacaa agggtacatc agttggcagt acaattacat
     8521 ttagagggat tcttaaacaa gtatccttct gaaatctctg ggggtcagaa gcaacgcatt
     8581 gccattgcac gtgcattagt tactaagccg acgattttac tagccgatga acctacaggt
     8641 gcacttgatt caaaaacgtc aaaggcattg atgatgttat ttcaagagat tcatcaattg
     8701 gaacagacaa ttttaatggt aactcattca aatatcgatg cgtcttatgc agagcgagtc
     8761 atttttatta aagatgggcg tctatatcat gaaatatatc gtggtgaaga aagtcaatta
     8821 gcttttcaac aacgaataac agatagctta gcacttgtga atggaggaag tgtcaatata
     8881 tgaagttaag attgttatgg cacatagtgc gacgtcaatt tattacgcag cgacttgtaa
     8941 tcattccatt cattttagcg gtaagtgtac tattcatgat tgaatatacg cttgtgtcaa
     9001 ttgggttaaa tagctacata aaacagaaga atgacttcct agtaccattt attatcatag
     9061 ctaatttttt tatggcgctt ttaactttta tttttatttt ctatgcaaat cactttatga
     9121 tgtcacaaag acgaaaagag tttagcattt ttatgacatt gggcatgacc aagaaaagta
     9181 tgcgtttaat tgtagtgatg gaaactatct tacaatttgt gataatttca gtcgttagta
     9241 ttgccggcgg atacttactt ggtgcgatat ttttcttgtt tatacagaaa ataatgggca
     9301 gtgaagttgc gacgttaagg tattatccat ttgactctgt agcgatgttt attactttga
     9361 ttatcattgc tgtattaatg ggcatgctac ttatattcaa cttgttcagt attaattttc
     9421 aacggccgat aacttatcaa catcgttccg attctagtgt catatcacga tggttgcgtt
     9481 acgttttaat tgttatagga agcgcagcac tatatttagg ttactttatt gcattacaac
     9541 aagatacgac gtttggtgcc ttttttaaaa tatggattgt cataggatta gttattatcg
     9601 gtacttatgc attttttgta ggtataagtg aaataattat tagtatattg cagcaggtat
     9661 caaaagttta ctatcatcca cggtattttt ttgtggtagt tgggatgcgt gtacgtctta
     9721 aaatgaatgc agtcagtctt gcaacaatca ctttgctgtg tacatttttg attgtaacgc
     9781 tcacaatgac attaacaacc tatcgtgata tgaatcatac cattacgaaa ttgattacga
     9841 atgattatga tttgtcattt agcgacaatt ctaagtcaca aatagaacgt caacaaacaa
     9901 ttgagaatat taaacgtgat atacagggtt cggttaatgc aaaagacttc aaagtatatg
     9961 aaacgacatt atttagagct tctttagaaa agaatcgtgc ttattacaag ttaaaacagg
    10021 ctagtgatga tattccaata gacttcattg gtaatgaagg cgcgatattt tcaagacaat
    10081 cagtaatgat tacagctttt actgcacaag attataatcg atatcaccaa aacaaattac
    10141 acttagacaa tcaaagtata ggtatgatta cgaatgtacc tatttttaaa aagcagtcta
    10201 aggtcggtct taataatgaa gtctttaaag tgaaacaact tgacgcacat gcgttaaatt
    10261 tgatgatgat tcaagatagt atggttttaa ttgttaaaga taacaatcaa ttacaaaaag
    10321 taaaaggatt ttatgcacat gaacaaaaga attcgacaat atcgataaac tttaatgtat
    10381 ttggaaagac gaaattaaca acatcacaaa tacaaaagct atcgaaaaaa tacgatgcgt
    10441 caataaattc taaatcagaa atgctaatgg tttgggatcg tttgacaggt ggactaatat
    10501 ttgtaggtgg cgtagtatca attgtacttg tcattggaat atttctaatg atgtactata
    10561 aacaagtctc agaagcctat gcaaatcagc acaattatga cattatgaaa aaacttggtt
    10621 tagataatgg aagaatagca aagataacac gaaatcaaat gactttcttg ttcgcaatac
    10681 caataacagt agcactgatt cacacattaa tttctagtaa cattgtatat acgttactaa
    10741 atatgatagg tattaataac catcatatat ttttaacaag ttacgtacta gctgtcatta
    10801 taatttcgtt tctatatatg gcgatgtaca aaataacttc ctatatttat gctaaagtta
    10861 ttcatcaaca acgtaattaa ccatgtttca gtgaaatcac aatcaatcat gtattttcac
    10921 tgaaatatat tatattaaaa agaaacctaa aaacattaca aataacaata ccaaattt
//
LOCUS       DANBWH010000086        10692 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.089, whole genome shotgun sequence.
ACCESSION   DANBWH010000086 DANBWH010000000
VERSION     DANBWH010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10692
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.089"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            117..533
                     /locus_tag="PPY92_001498"
     CDS             117..533
                     /locus_tag="PPY92_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDJ2625504.1"
                     /translation="MKTKEVVALMNISQDTLRYYEKVGVIPPVNRDENGYRIYNDSDL
                     NWIYLVKNLRNAGVSIESLIEFCRLAQLPKNKNIQTQQKQILNKQLEELNENLKTIHD
                     ARDLLKYKIDNYDNHIAKINASDNHDDNVERLWERK"
     gene            603..1451
                     /locus_tag="PPY92_001499"
     CDS             603..1451
                     /locus_tag="PPY92_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDJ2625505.1"
                     /translation="MNHIEISKDVKIPVLGFGVFQIPQEQTAEAVKEAIKAGYRHIDT
                     AQSYLNETEVGQGIEASGIDRSELFITTKVWIENVNYEDTIKSIERSLQRLNLDYLDL
                     VLIHQPYNDVYGSWRALEELKENGKIKAIGVSNFGVDRIVDLGIHNQIQPQVNQIEIN
                     PFHQQEEQVAALQQENVVVEAWAPFAEGKNQLFQNQLLQAIADKYNKSIAQVILRWLV
                     ERDIVVLAKSVNPERMAQNLDIFDFELTEEDKQQIATLEESNSQFFSHADPEMIKALT
                     SRELDV"
     gene            1684..1887
                     /locus_tag="PPY92_001500"
     CDS             1684..1887
                     /locus_tag="PPY92_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625506.1"
                     /translation="MKYKHFAIITLITGIVMLTAMYFVPFGYHKIPIFAGMAIIFTVY
                     FSNKKSIDETNIFSKKNRNKNKQ"
     gene            complement(1974..2135)
                     /locus_tag="PPY92_001501"
     CDS             complement(1974..2135)
                     /locus_tag="PPY92_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625507.1"
                     /translation="MLLILELRFWNLEFGFRNSTFGIQNYNEWKILIIACLMTWFCSD
                     FYLMNCFFV"
     gene            complement(2172..2912)
                     /locus_tag="PPY92_001502"
     CDS             complement(2172..2912)
                     /locus_tag="PPY92_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDJ2625508.1"
                     /translation="MFILRNDLETLKHIIDSSNRITFFTGAGVSVASGVPDFRSMGGL
                     FDEISKDGLSPEYLLSRDYLEDDPEGFINFCHKRLLFVDTKPNIVHDWIAKLERNQQS
                     LGVITQNIDGLHSDAGSQHVDELHGTLNRFYCNACHKSYTKSDVIDRTLKHCDNCGGA
                     IRPDIVLYGEMLDQPTIIRALNKIEHADTLVVLGSSLVVQPAAGLISHFKGDNLIIIN
                     KDRTPYDSDATLVIHDDMVSVVKSLMTE"
     gene            3118..3909
                     /locus_tag="PPY92_001503"
     CDS             3118..3909
                     /locus_tag="PPY92_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDJ2625509.1"
                     /translation="MTHRLNKGGNFTLNKHERLDEIAKLVNKKGTIRTNEIVEGLNVS
                     DMTVRRDLIELENKGILTKIHGGARSNSTFQYKEISHKEKHTRQIAEKRYIARKAASL
                     IEDGDTLFFGPGTTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMR
                     HITEAFVGEMANAMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYL
                     LIDHTKVGKEDFTSFCQLNELTAVVMDYEDEEKVETIKTYIEVVD"
     gene            4276..4704
                     /gene="lacA"
                     /locus_tag="PPY92_001504"
     CDS             4276..4704
                     /gene="lacA"
                     /locus_tag="PPY92_001504"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HDJ2625510.1"
                     /translation="MAIIIGSDEAGKRLKEVIKSYLLDNKYDVVDVTEGQEVDFVDAT
                     LAVAKDVQSQEGNLGIVIDAFGAGSFMVATKIKGMIAAEVSDERSGYMTRGHNNSRMI
                     TMGSEIVGDTLAKNVVKGFVEGKYDGGRHQIRVDMLNKMC"
     gene            4720..5235
                     /gene="lacB"
                     /locus_tag="PPY92_001505"
     CDS             4720..5235
                     /gene="lacB"
                     /locus_tag="PPY92_001505"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HDJ2625511.1"
                     /translation="MKIALGCDHIVTDTKMRVSEFLKSKGHEVIDVGTYDFTRTHYPI
                     FGKKVGEQVVSGNADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSALYAKEELNAN
                     VIGFGGRIIGELLMCDIIDAFINAEYKATEENKKLIAKIKHLETSNADQADPHFFDEF
                     LEKWDRGEYHD"
     gene            5248..6180
                     /gene="lacC"
                     /locus_tag="PPY92_001506"
     CDS             5248..6180
                     /gene="lacC"
                     /locus_tag="PPY92_001506"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HDJ2625512.1"
                     /translation="MILTLTLNPSVDISYPLTALKLDDVNRVQEVSKTAGGKGLNVTR
                     VLAQVGEPVLASGFIGGELGQFIAKKLDHADIKHAFYNIKGETRNCIAILHEGQQTEI
                     LEQGPEIDNQEAAGFIKHFEQLLEKVEAVAISGSLPKGLNQDYYAQIIERCQNKGVPV
                     ILDCSGATLQTVLENPYKPTVIKPNISELYQLLNQPLDESLESLKQAVSQPLFEGIEW
                     IIVSLGAQGAFAKHNHTFYRVNIPTINVLNPVGSGDSTVAGITSAILNHENDHDLLKK
                     ANTLGMLNAQEAQTGYVNLNNYDELFNQIEVLEV"
     gene            6184..7164
                     /gene="lacD"
                     /locus_tag="PPY92_001507"
     CDS             6184..7164
                     /gene="lacD"
                     /locus_tag="PPY92_001507"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDJ2625513.1"
                     /translation="MSKSNQKIASIEQLSNNEGIISALAFDQRGALKRMMAKHQTEEP
                     TVAQIEQLKVLVAEELTQYASSILLDPEYGLPASDARNKDCGLLLAYEKTGYDVNAKG
                     RLPDCLVEWSAKRLKEQGANAVKFLLYYDVDDAEEINIQKKAYIERIGSECVAEDIPF
                     FLEVLTYDDNIPDNGSVEFAKVKPRKVNEAMKLFSEPRFNVDVLKVEVPVNMKYVEGF
                     AEGEVVYTKEEAAQHFKDQDAATHLPYIYLSAGVSAELFQETLKFAHEAGAKFNGVLC
                     GRATWSGAVQVYIEQGEDAAREWLRTTGFKNIDDLNKVLKDTATSWKQRK"
     gene            7186..7497
                     /locus_tag="PPY92_001508"
     CDS             7186..7497
                     /locus_tag="PPY92_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDJ2625514.1"
                     /translation="MNREEVQLLGFEIVAFAGDARSKFLEALTAAQAGDFAKADALIE
                     EGNNCIAEAHRAQTSLLAKEAQGDDIAYSVTMMHGQDHLMTTILLKDLMKHLLEFYKR
                     G"
     gene            7503..9215
                     /locus_tag="PPY92_001509"
     CDS             7503..9215
                     /locus_tag="PPY92_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDJ2625515.1"
                     /translation="MMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSI
                     FLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVAFLVAGTTAKSLTDSFNRKLESTN
                     QINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKR
                     NITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLF
                     TAADGWIGVTIIFGAFALFWFVGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKI
                     ITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVVPTFFGVNEPILFGAP
                     LVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWS
                     FVLAITLIVVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILA
                     ASGVSDDAAKASNITEQTNVLVLCAGGGTSGLLANALNKAAEEYHVPVKAAAGGYGAH
                     MDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQAALDFVQQ
                     QFEN"
     gene            9233..10645
                     /gene="lacG"
                     /locus_tag="PPY92_001510"
     CDS             9233..10645
                     /gene="lacG"
                     /locus_tag="PPY92_001510"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDJ2625516.1"
                     /translation="MTKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWY
                     TAEPASDFYNRYPVDLELSEKFGVNGIRISIAWSRIFPNGYGEVNPKGVEYYHKLFAE
                     CHKRHVEPFVTLHHFDTPEVLHKDGDFLNRKTIDYFVDYAEYCFKEFPEVKYWTTFNE
                     IGPIGDGQYLVGKFPPGIKYDFEKVFQSHHNMMVAHARAVKLFKDGGYQGEIGVVHAL
                     PTKYPFDPSNPEDVRAAELEDIIHNKFILDATYLGKYSRETMEGVQHILSVNGGKLNI
                     TDEDYAILDAAKDLNDFLGINYYMSDWMRGYDGESEITHNATGDKGGSKYQLKGVGQR
                     EFDVDVPRTDWDWMIYPQGLYDQIMRVVKDYPNYHKIYITENGLGYKDEFIESEKTVH
                     DDARIDYVRQHLNVIADAIKDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQE
                     RYPKKSAYWYKELAETKEIK"
BASE COUNT         3859 a         1573 c         2020 g         3240 t
ORIGIN      
        1 taaaaaagag acaaatttaa atttgagtaa taaaataaga agtattcaaa caaagatggc
       61 aatatataaa acaacattac tttagatgcc caaatataac tgaaaggata gtcgcaatga
      121 aaactaaaga agtcgtagcg ctcatgaata tatctcaaga cactttaaga tattatgaaa
      181 aggttggtgt gattccaccc gttaatcgag atgaaaatgg atatagaata tataatgata
      241 gtgatttaaa ttggatttat ttagtgaaaa atttgcgaaa tgcaggcgtc agtattgaat
      301 cgcttatcga attttgtaga ttagcgcagc ttcctaaaaa taaaaatatt caaacacagc
      361 aaaagcaaat tttaaataag caactcgaag aattaaatga aaacttaaag acaattcatg
      421 atgcgagaga tttactaaaa tataaaattg ataattatga taatcatatt gctaaaatca
      481 atgctagtga taatcatgat gacaatgttg aacgtctttg ggagagaaag taaacctctg
      541 gcttgctata gagttcgctc catggtttat agtggacata caagataaag gaggttcatt
      601 ttatgaatca tattgaaata agtaaagatg taaagatacc agtattaggt ttcggagtat
      661 ttcaaattcc tcaagaacaa acagcagaag cggttaaaga agcaattaaa gcgggttata
      721 gacatattga tacagcacaa agttatttaa atgaaactga agtaggtcaa ggtattgaag
      781 cttcaggtat tgatagaagt gaactattca ttacgactaa agtatggatt gaaaatgtta
      841 actatgaaga tactatcaaa tcaatcgaac gatcattaca acgattaaat ttggactatc
      901 ttgatctagt gcttattcac caaccttaca atgacgtata tggctcatgg cgtgcattag
      961 aagagttaaa agaaaatggt aaaattaaag caatcggtgt atctaacttc ggcgttgatc
     1021 gaattgtaga tttaggtatt cacaatcaaa ttcaaccaca agtgaaccaa atagaaatta
     1081 atccattcca tcaacaagaa gaacaagttg cagcacttca acaagaaaat gttgtggtag
     1141 aagcttgggc accatttgca gaaggtaaaa atcaattatt ccaaaatcaa ttgttgcagg
     1201 ccattgcaga taaatacaac aaatcaattg cacaagttat tttacgctgg ttagtagaac
     1261 gtgacattgt tgtactcgca aaatcagtga atccagaacg tatggctcaa aacttagata
     1321 tatttgattt tgaacttaca gaagaagata aacaacaaat tgcgacatta gaagaaagta
     1381 atagccaatt tttctcacat gctgatccag agatgattaa agcacttaca agcagagaat
     1441 tagatgttta atcataatat aacaaagtaa ataatttaca ttacgctaac ctgaaacttg
     1501 gatgttaaag tctatgttgc aggttatttt attactctta aaaagtgaat ggagcaagca
     1561 aataagttat tatgaatttt tgtattatgc aataacttac ctaagataat aattatagta
     1621 ttgataattt tataaaatga agtatataat gaatgtagaa taaattgtat aggggataat
     1681 aacatgaaat ataaacattt tgccattatt actttaatta caggcatcgt catgctcacg
     1741 gcaatgtact ttgtaccatt tggatatcat aaaataccaa tatttgcagg aatggcgatt
     1801 atttttacag tctattttag taataaaaaa tctattgatg aaacaaatat ctttagtaag
     1861 aaaaatcgca ataaaaataa acaataaaat gagttgtaag cataagattt acagttaatt
     1921 ttttacgaat gggcctttag cgattgaaaa tgttataaag tagcatccca acattaaaca
     1981 aaaaaacaat tcatcaaata aaaatcgcta caaaaccaag tcattaaaca cgcaataatt
     2041 aaaattttcc actcattata attctgaatt ccaaatgtcg aattccgaaa accaaactcc
     2101 aaattccaaa aacgcaactc caaaattaaa agcatctccc tgttactcgg gaaatgcttt
     2161 ttacctactg attactctgt cattaatgat tttacaacgg aaaccatgtc gtcatgtatg
     2221 accaaagtag cgtcgctatc ataaggtgtt cgatctttat tgataataat taaattgtcg
     2281 cctttaaaat gtgatattaa tcctgcggca ggttgtacaa cgagtgatga acctagtaca
     2341 acaagggtgt cagcatgttc aattttattt aatgccctta tgatggtagg ttgatctaac
     2401 atttcaccgt ataatacgat gtccggtcga atggcaccac cacaattatc acaatgtttc
     2461 aaagttctat caataacatc tgacttcgta taagatttat gacatgcatt acaataaaaa
     2521 cgatttaacg tgccatgtaa ttcatcaaca tgttgacttc cagcgtctga gtgcaaacca
     2581 tcgatatttt gcgtgatgac acctaaagat tgttgattac gttctaattt tgcaatccaa
     2641 tcatgaacga tattaggctt cgtatcgaca aatagtaaac gcttatggca gaaattgata
     2701 aaaccttcag gatcatcttc taaataatca cggcttaaca agtattctgg cgaaagtcca
     2761 tcttttgaaa tttcatcaaa taagccaccc attgaacgga aatctggaac gccacttgcg
     2821 acagatacac cagcacctgt gaaaaatgta atacgattcg aactatctat aatatgtttt
     2881 aatgtctcta aatcatttct caaaataaac accccatcaa aataatgaat gatttataac
     2941 gtcttttgct tttattatag tatattcatt ttacgattat caaaagcgca gtttaaacag
     3001 ttctttttaa aaatgaatta tacatataat cgaacagtta aattatatta gtttttacgc
     3061 aatttttatt atagaataag catgtatatt aaaattgatg tctaaacata aaggataatg
     3121 acccatcgcc taaataaagg aggcaatttt acattgaata aacatgaacg tttggacgaa
     3181 attgctaaac tagtgaataa aaagggcacg ataagaacga atgaaatcgt cgaaggttta
     3241 aatgtgtctg atatgacagt tcgaagagat ttaattgagt tggaaaataa gggcatttta
     3301 acgaagattc atggtggtgc acgcagtaat tcaacatttc agtataaaga aatatcacat
     3361 aaagaaaaac atactcgtca aattgctgaa aaaagatata tagctaggaa agctgcatca
     3421 ttaattgaag atggggatac tttgtttttc ggaccgggaa caacagtgga actattagca
     3481 gaagaagtca atcatcatac gctcacaatt attacgaatt gtttgccggt gtataaaatt
     3541 ttgttagaaa aacaaacagc acattttcgt gtctatttaa ttggtggtga aatgcgccat
     3601 attacagaag catttgtagg tgaaatggcg aatgccatgt tggaaaaact aagatttagc
     3661 aagatgttct ttagtagtaa cgcagtaaat aaaggtgctg tgatgacatc tacattagat
     3721 gaagcctata cgcaacaatt ggcactaagt aattcaattg aaaaatactt gttaatcgat
     3781 catacgaaag ttggcaaaga agattttaca tcattttgtc agttaaacga attgactgca
     3841 gtggtcatgg actatgaaga tgaagaaaaa gtagaaacga ttaaaacata tattgaagta
     3901 gtcgactaaa ttaaaagtat aactgcattg ttaaaatatg tatctatatg ctcataagtg
     3961 aatgtgaatt tgcattcatt tatgggttat ttttttgcaa atttatgtaa acgggaacat
     4021 cgacaaagtt ttactacaat ttcagacgaa tacctaatta aaacaacacc acaataggaa
     4081 tgttcactta ttgtttgaaa atatgtcata aacaaacagg ttataaacat attatgttga
     4141 tttattgttt gtttatgttt ataatttaaa cgtaatcaaa ttgaaagcct tttctcaaga
     4201 ttttcataag ctacaaataa atatgtcata ttttaagaaa ggtatttcaa aattaaagta
     4261 aaaaggagtc ttattatggc gattattatt ggttcagatg aagctggcaa acgattaaaa
     4321 gaagtcatca aatcatactt attagacaat aaatatgatg ttgttgacgt aacagaagga
     4381 caggaagttg actttgttga tgcaactttg gctgtagcaa aagatgttca aagtcaagaa
     4441 ggtaacttag gtattgttat cgatgcattt ggtgcaggta gctttatggt tgctacaaaa
     4501 atcaaaggca tgattgctgc tgaagtatca gatgaacgct ctggatatat gactagaggc
     4561 cataacaatt caagaatgat cactatggga tctgaaattg ttggagacac attagcgaaa
     4621 aatgtcgtta aaggttttgt tgaaggtaaa tacgatggtg gtagacacca aatccgcgta
     4681 gatatgctta ataaaatgtg ctaaggagga aaaattacta tgaagattgc attaggatgc
     4741 gaccatattg ttacagatac aaaaatgcgt gtatctgaat ttttaaaatc aaaaggacat
     4801 gaagtcattg acgtaggaac atacgatttt acaagaacac attatccaat ttttggtaaa
     4861 aaagttggcg aacaagttgt tagcggtaat gcagacttag gtgtttgtat ttgtggaaca
     4921 ggtgttggta ttaacaatgc tgtaaataaa gtacctggcg ttcgttcagc actagtacgt
     4981 gatatgacat cagcgttata tgctaaagaa gaattaaatg cgaacgttat tggctttggt
     5041 ggacgtatta taggtgagtt attaatgtgc gatattatcg atgcatttat taacgctgaa
     5101 tataaagcaa ctgaagagaa caaaaaatta atcgctaaaa ttaaacattt agaaacaagc
     5161 aatgcagatc aagctgatcc acatttcttt gatgaattct tagaaaaatg ggacagaggc
     5221 gaataccacg attaagggac gacacatatg attttaactt taacattaaa tccatccgtt
     5281 gatatttcat atccattaac agcattgaaa ttagatgatg ttaatcgtgt tcaagaagta
     5341 agtaaaacag ctggtggtaa aggtttaaat gtaacaagag tacttgcgca agtaggcgag
     5401 ccagtccttg cgagtggttt tattggcggt gaattaggtc aatttattgc taaaaaatta
     5461 gatcatgccg acatcaagca tgcattttat aacattaagg gtgaaacacg aaactgcatt
     5521 gcaattttac atgaagggca acaaacagaa attttagagc agggaccgga gattgataat
     5581 caagaggctg caggatttat taaacatttt gaacaattat tagaaaaagt tgaagcagtt
     5641 gctatttcag gtagcttacc taaaggatta aatcaagatt attatgcaca aattattgaa
     5701 cgatgccaaa acaaaggtgt gcctgtgata ttggactgct cgggtgcaac tttgcaaaca
     5761 gttttggaaa atccatacaa accaacagtc attaaaccaa acatttctga actatatcaa
     5821 ttgcttaacc agccacttga tgaaagttta gaaagtttga aacaggcagt gtcacaaccc
     5881 ttattcgaag ggattgagtg gattattgtg tctttaggtg cacaaggtgc attcgctaag
     5941 cacaatcata cattctatag agtgaatatt ccgacaataa acgtattaaa tccggttggt
     6001 tcaggtgatt ctacagttgc tggaattact tcagcaattc tcaatcatga aaacgaccac
     6061 gatttactaa aaaaagctaa tactttaggc atgctgaatg cgcaagaagc acaaacagga
     6121 tatgtcaatt taaataatta tgatgaatta ttcaatcaaa ttgaagtttt agaggtgtaa
     6181 ataatgtcaa aatctaatca aaaaatcgca tcaattgaac aattaagtaa taacgaaggt
     6241 atcatttctg cattagcatt tgaccaacgt ggtgctttaa aacgaatgat ggctaaacat
     6301 caaacggaag agccaactgt cgctcaaatt gaacaattaa aagtattggt tgcggaagaa
     6361 ttaacacaat atgcgtcttc aattttatta gatccagaat acggtttacc agcatctgat
     6421 gcacgtaaca aagattgtgg attattgcta gcttatgaaa aaactggtta tgacgtaaat
     6481 gctaaaggtc gtttacctga ctgtttagta gagtggtctg caaaacgttt aaaagaacaa
     6541 ggtgcaaatg ctgttaaatt cttactttac tatgatgttg atgatgctga agaaattaac
     6601 attcaaaaga aagcatacat tgaaagaatc ggttcagaat gtgttgcaga agacattcct
     6661 ttcttcttag aagtattaac atacgatgac aacattcctg ataacggtag tgttgaattc
     6721 gcaaaagtta agcctagaaa ggttaacgaa gcaatgaaat tattctcaga accacgcttt
     6781 aatgttgatg tattgaaagt tgaagtacca gtaaacatga agtatgtaga aggctttgct
     6841 gaaggtgaag tggtttatac aaaagaggaa gcggcacaac actttaaaga tcaagatgca
     6901 gcaactcatt taccatacat ttacttaagt gctggtgttt ctgcggaatt attccaagaa
     6961 actttaaaat ttgctcacga agctggtgct aaattcaacg gtgtactttg tggccgtgct
     7021 acatggtctg gtgctgttca agtatacatt gaacaaggtg aagatgctgc tcgtgaatgg
     7081 ttacgtacaa ctggtttcaa aaatattgat gacttaaata aagtattaaa agatactgcg
     7141 acatcttgga aacaaagaaa ataagtataa aaggggatta atgctatgaa tagagaagaa
     7201 gtccaattat taggttttga aattgttgca tttgcagggg atgcacgttc taagttttta
     7261 gaagcattga cagcagctca agctggagat tttgcaaaag cagatgcatt gattgaagaa
     7321 ggaaacaatt gcattgctga agcgcataga gcacaaacaa gcctgttagc taaagaagcg
     7381 caaggtgatg atattgcata cagtgtaacg atgatgcatg gtcaagacca tttaatgaca
     7441 acaattttac tgaaagattt aatgaagcat ttattagaat tttataaaag agggtgatga
     7501 caatgatgca aaaattgatc gcacaaattg aaaaagggaa gccatttttc gagaaattat
     7561 cacgtaacat atatttaaga gccatccgtg atggatttat ttctgcaatg cctgtcatct
     7621 tattctcaag tatctttcta ttaattgcat acgtgccaaa tatatttggt tttaaatggg
     7681 ataaaggcat ggaagccatt ttaatgaaac catataacta tacgatgggg cttgtagcat
     7741 ttttagtagc aggaacgaca gcgaaatctt taacagattc atttaaccgt aagctagaaa
     7801 gtacaaatca aattaacttt atttctacaa tgctagcagc gatgtgtgga ttcttgttct
     7861 tagcttctga tccagccaaa gatggtggat tcttaagtgc atttatggga actaaaggtt
     7921 tattaacagc atttttatca gcatttgtaa ctgttattgt ttataacttc tgtgtgaagc
     7981 gcaatattac aattaaaatg ccgaaagaag taccgccgaa tatttcacaa gtatttaagg
     8041 acttaattcc attttcagcg gtaatcatca ttctttatgc attagattta gtcattcgca
     8101 acagctttaa atcaaatgta gcggaaggta ttttaaaatt attcgaacca ttatttacag
     8161 cagcagatgg atggattggt gtcacaatta tctttggtgc ctttgcatta ttctggtttg
     8221 taggaattca tggtccatca atagtagagc cagcaattgc agccattaca tatgcgaata
     8281 tcgaagcgaa cttcaagttg cttcaagctg gagaacacgc agataaaatt attacatcag
     8341 gtacacaaat gtttatcgtt acttttggag gtacgggtgc aacattggtc gtcccgttca
     8401 tgtttatgtg gatgacgaaa tctaaacgaa acaaagctat tggacgtgca tcagttgtac
     8461 caacattctt tggtgtaaac gaaccaattt tatttggtgc accactagta ttgaatcctg
     8521 tgttctttat tccatttgta ttagcaccaa ttgttaacgt atggattttc aaacttttcg
     8581 ttgaagtgtt aggaatgaat agttttagtg taaatttacc ttggacaaca ccaggtccat
     8641 tgggcattat catgggtaca ggttttggtt tatggtcatt cgtactagct attactttga
     8701 ttgttgtaga tattattatt tactacccat tcctaaaagt ttatgatagt gaaattcttg
     8761 atgaagaaga aggacgtaaa gaaagtaatt cagatttaaa agaaaaagtt gcagcaaact
     8821 ttgatacgaa aaaagctgat tcaattttag cggcaagtgg tgtatcagac gatgcagcta
     8881 aagcttcaaa tatcacagaa caaacaaatg tcttagtact ttgtgccggt ggtggtacaa
     8941 gtggattatt agccaacgca cttaataaag cagctgaaga atatcatgtg ccagtgaaag
     9001 cagcagctgg cggatacggc gcacatatgg acattatgaa agagtaccaa ttaatcattt
     9061 tagcgccaca agttgcgtct aactatgaag atattaagca agatacagat agattaggta
     9121 ttaaattagc gaagactcaa ggtgctgaat atatcaaatt gacacgagac ggccaagcag
     9181 cccttgattt cgttcaacaa caatttgaaa attaagaata ggagtttttc atatgactaa
     9241 aacattacct gaagatttta tttttggtgg agcaacagca gcatatcaag cagaaggtgc
     9301 gacaaataca gatggtaaag gtcgtgttgc atgggatacg tatttggaag aaaactattg
     9361 gtacactgca gaaccagcga gtgattttta taacagatat ccagtggatt tagaattaag
     9421 tgaaaaattc ggtgttaatg gtatccgtat ttcaattgca tggtcacgta tcttcccaaa
     9481 tggatatggc gaagttaacc ctaaaggtgt tgagtattac cataagttat ttgcagagtg
     9541 tcataaacgt catgttgaac catttgtaac attgcatcat tttgatacac cagaagtgtt
     9601 acacaaagat ggcgattttc taaatcgtaa aacgattgac tactttgtag attatgctga
     9661 atattgtttt aaagaattcc cagaagtaaa atattggact acattcaatg aaattggacc
     9721 tattggtgat ggtcaatatt tagtaggtaa attccctcca ggtattaaat atgattttga
     9781 aaaagttttc caatcacatc acaatatgat ggttgctcat gcaagagcag taaaattatt
     9841 taaagatggc ggctatcaag gagaaattgg tgttgtacac gcattgccaa caaaatatcc
     9901 gttcgatcca tcaaatcctg aagatgttcg tgcagcagaa ttagaagata tcatccataa
     9961 taaattcatc ttagatgcaa catatttagg taagtattct cgtgaaacga tggaaggtgt
    10021 gcaacatatc ttatctgtta atggcggtaa attaaatatt acagatgaag attatgccat
    10081 tttagatgca gccaaagatt taaacgactt cttaggtatc aattactaca tgagtgactg
    10141 gatgagaggt tacgatggag aatctgaaat cacgcataat gcgacaggtg ataaaggcgg
    10201 ttctaaatac caacttaaag gtgtcggaca acgtgaattt gatgtcgacg tacctcgcac
    10261 agactgggac tggatgatct atcctcaagg tttatatgat caaatcatgc gtgttgttaa
    10321 agattatcct aactatcaca agatttatat cactgaaaat ggtttaggat ataaagatga
    10381 atttattgaa tctgaaaaaa cagttcatga tgatgcacgt attgattatg taagacaaca
    10441 tttgaacgtt attgcagatg ctattaaaga tggtgcaaat gttaaaggtt actttatttg
    10501 gtcattaatg gatgtgttct cttggtcaaa tggttatgaa aaacgatatg gtttattcta
    10561 tgtcgatttt gagactcaag aacgttatcc taagaaaagt gcttattggt acaaagagtt
    10621 agccgaaaca aaagaaatta aataattata gatattaaaa ggcctatcag tcgagtgctg
    10681 gtaggtcttt at
//
LOCUS       DANBWH010000087        10680 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.090, whole genome shotgun sequence.
ACCESSION   DANBWH010000087 DANBWH010000000
VERSION     DANBWH010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10680
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.090"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(149..892)
                     /locus_tag="PPY92_001511"
     CDS             complement(149..892)
                     /locus_tag="PPY92_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDJ2625517.1"
                     /translation="MTLNKLKDELQIVSHRGLPSDFPENTMVGYREVMGLNVAMLEID
                     VHLTKDQHFVVIHDETIDRTSDGKGRIADYTLSQLKSFDFGSYKDVAFKGERIPTLDE
                     VLSLCLKYDKKLLIELKSPNLYPEIECKLLAFLEEKKVDATQVVIQSFDIECIEKLNT
                     LGSIYELGVLSSKRNYWYKKPNFSRIAQIASYVNPNYALVARKFVDKAHHHQLQVMPY
                     TVNKLKTGEKLRQMGVDGLITDNPKLFIK"
     gene            972..1418
                     /locus_tag="PPY92_001512"
     CDS             972..1418
                     /locus_tag="PPY92_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HDJ2625518.1"
                     /translation="MHQHDFKVQTSWQGGRNNVGNVQGDILSENISIPASLGGVGIGT
                     NPDEMLVSAASSCYIISLAATLERAKFTDISIEQQSIGTACLNNGKFSMSKIVHHPHI
                     EIPSDQIAQLEKRLPKLITIADNNCMISNAVRNNVDIKIYPIIQAK"
     gene            1533..2690
                     /locus_tag="PPY92_001513"
     CDS             1533..2690
                     /locus_tag="PPY92_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDJ2625519.1"
                     /translation="MYYHQPLLLTPGPTPVPDAIMREIQAPMVGHRSKDFEDIAQQAF
                     QGLKPIFGSQNDVLILTSSGTSVLEASMLNIVNPEDHFVVIVSGAFGNRFKQIAQTYY
                     KNVHIYDVTWGEAVDVKDFINYLSTLDVEVKAVFSQYCETSTTVLHPIHELGNAINQF
                     NSNIYFVVDGVSCIGAVDVDINKDKIDVLVSGSQKAIMLPPGLAFVAYSHRAKERFKE
                     VTTPKFYLDLNKYISSQADNSTPFTPNVSLFRGVNAYVETVKAEGFNHVIARHYAIRN
                     ALRSALKALDLTLLVNDKDASPTVTAFKPNTNEVKIIKDELKNRFKITIAGGQGHLKG
                     QILRIGHMGKISPFDILSVVSALEIILTEHRKVNYIGKGISKYMEVIHEAI"
     gene            2677..4281
                     /locus_tag="PPY92_001514"
     CDS             2677..4281
                     /locus_tag="PPY92_001514"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDJ2625520.1"
                     /translation="MKQFNVLVADPISKDGIKALLDHEQFNVDIQTGLSEEALIKIIP
                     SYHALIVRSQTTVTENIINAADSLKVIARAGVGVDNININAATLKGILVINAPDGNTI
                     SATEHSLAMLLSMARNIPQAHQSLTNKEWNRNAFKGTELYHKTLGVIGAGRIGLGVAK
                     RAQSFGMKILAFDPYLTDEKAKSLSITKATVDEIAQHSDFVTLHTPLTPKTKGLINAD
                     FFAKAKPSLQIINVARGGIIDEKALIKALDEGQISRAAIDVFEHEPATDSPLVAHDKI
                     IVTPHLGASTVEAQEKVAISVSNEIIEILIDGTVTHAVNAPKMDLSNIDDTVKSFINL
                     SQTVGELAIQLMYNAPSSIKITYGGDLASIDSSLLTRTIITHILKDDLGPEVNIINAL
                     MLLNQQQVTLNIENNKAETGFSNYLEVELSNDSDSVKVGASVFTGFGPRIVRINNFSV
                     DLKPNQYQIVSYHNDTPGMVGKTGALLGKYNINIASMTLGRTEAGGDALMILSVDQPV
                     SNNIIDELKQVGEYNQIFTTELTVQS"
     gene            4375..4470
                     /locus_tag="PPY92_001515"
     CDS             4375..4470
                     /locus_tag="PPY92_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625521.1"
                     /translation="MKPLVQSFTSSYAVKQQSNYKLSSTPLSSPK"
     gene            complement(4431..5561)
                     /locus_tag="PPY92_001516"
     CDS             complement(4431..5561)
                     /locus_tag="PPY92_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDJ2625522.1"
                     /translation="MKKILFDVDGIFLSEERCFDVSALTVYELLMDKCYLGLHSHIDW
                     ETLTDNDIQDIRNRIFQKDKILNKLKSLGLNSNWDMLFIVFSIHLIDILKKLSHDEIE
                     AFMYQDEPVELKLQNISTNLADCFNLNEQLPLQFLDNVKVGKNNIYAALEEFATTELH
                     VSDATLFSLKGALWTLAQEVYQEWYLGSKLYEDVEKKIARTTFKTGYIYQEIILRPVD
                     EVKVLLNDLKGAGFELGIATGRPYTETVVPFENLGLLPYFEADFIATASDVLEAENMY
                     PQARPLGKPNPFSYIAALYGNNRDKYESYINKQDNIVNKDDVFIVGDSLADLLSAQKI
                     GATFIGTLTGLKGKDAAGELEAHHADYVINHLGELRGVLDNL"
     gene            complement(5668..7134)
                     /locus_tag="PPY92_001517"
     CDS             complement(5668..7134)
                     /locus_tag="PPY92_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIBC"
                     /protein_id="HDJ2625523.1"
                     /translation="MYKFFQNLGRSLMLPVAILPAAAIIAGIGNTLNALHATPKIAMF
                     FTTVGTTILEQLGILFAIGVAIGMAKKNDGAVALAATLGYFLVTVVLSPMKLAPLLGM
                     KASEINSAFEKMNNGNVFVGIVIGLIAAYAYNKFSETELPLALSFFSGKRLVPIMTAF
                     YCTFLVVILLFLWPLLYSWIVKFGESIVGLGSFGAFIYGVANRLLIPTGLHHALNSVF
                     WFDTIGINDIGKFQSGKDAIKGITGRYQAGFFPIMMFGIPAAALAMYHTAKTTQKKQV
                     YGWFLASSVAAFFVGVTEPIEFAFMFVAPILYVVHALLTGLSLFIAATFHWTAGFSFS
                     AGLIDYVLSLINPVSNHPLMLLVQGVVFFILYYVIFRVVIQVFNLNTIGRGENELVDP
                     TVVKDNIAPGENDIKQSKYHQHAIQILEGLGGQENIVNLTNCATRLRLELKDTSIIDQ
                     QKIKNAGAVGVTVNGKHSTQVIVGTHVQQVADEIEKHL"
     gene            complement(7262..7879)
                     /locus_tag="PPY92_001518"
     CDS             complement(7262..7879)
                     /locus_tag="PPY92_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDJ2625524.1"
                     /translation="MYSVISKILNFILVKMSKSLYVIGKDNIPKDSKYVVTCTHESYN
                     EVIMLGMALYPNQIHYMAKKELFKNKWIGKFLTSLNAFPVDRENPGPSTLKRPINLLK
                     DNKTVGIFPTGSRTSQEGAPLKRGASTIAMLSKSPILPVAYVGPTKIHGLLTGQAYIN
                     IGKPIDINDLPKDLKRNERIDYITKEIETRTAKLQQELYEIVKSL"
     gene            8051..9325
                     /locus_tag="PPY92_001519"
     CDS             8051..9325
                     /locus_tag="PPY92_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDJ2625525.1"
                     /translation="MSDFNHTDHSTTNHSQTPRYRRPKFPWFKTVIVALIAGIIGALL
                     VLGIGKVLNSTILNKDGSTVQTTNNKGGNQLDGQSKKFGTVHEMIKSVSPTIVGVINM
                     QKASSVDDLLKGKSSKPSEAGVGSGVIYQINNNSAYIVTNNHVIDGANEIRVQLHNKK
                     QVKAKLVGKDAVTDIAVLKIENTKGIKAIQFANSSKVQTGDSVFAMGNPLGLQFANSV
                     TSGIISASERTIDAETTGGNTKVSVLQTDAAINPGNSGGALVDINGNLVGINSMKIAA
                     TQVEGIGFAIPSNEVKVTIEQLVKHGKIDRPSIGIGLINLKDIPEEEREQLHTDREDG
                     IYVAKADSDIDLKKGDIITEIDGKKIKDDVDLRSYLYENKKPGESVTVTVIRDGKTKE
                     VKVKLKQQKEQPKRQSRSERQSPGQGDRDFFR"
     gene            complement(9419..10666)
                     /locus_tag="PPY92_001520"
     CDS             complement(9419..10666)
                     /locus_tag="PPY92_001520"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDJ2625526.1"
                     /translation="MEDLKWRGLIYQQTDEQGIEDLLNKEQVTLYCGADPTADSLHIG
                     HLLPFLTLRRFQEHGHRPIVLIGGGTGMIGDPSGKSEERVLQTEEQVDKNIEGISKQM
                     HNIFEFGTDHGAVLVNNRDWLGQISLISFLRDYGKHVGVNYMLGKDSIQSRLEHGISY
                     TEFTYTILQAIDFGHLNRELNCKIQVGGSDQWGNITSGIELMRRMYGQTDAYGLTIPL
                     VTKSDGKKFGKSESGAVWLDAEKTSPYEFYQFWINQSDEDVIKFLKYFTFLGKEEIDR
                     LEQSKNEAPHLREAQKTLAEEVTKFIHGEDALNDAIRISQALFSGDLKSLSAKELKDG
                     FKDVPQVTLSNDTTNIVEVLIETGISPSKRQAREDVNNGAIYINGERQQDVNYALAPE
                     DKIDGEFTIIRRGKKKYFMVNYQ"
BASE COUNT         3666 a         1912 c         1618 g         3484 t
ORIGIN      
        1 attatatgga gctaccctat tttacaatca acttagaatt tacaagtctt atttgtttaa
       61 ttatctttag tatttcatca ttagttttta aaattatcaa ttgcgatata cgaatcctca
      121 tccctctatc aaattaccat tcatcgtctc acttaataaa taatttcgga ttatctgtaa
      181 ttaatccatc tacacccatt tgtcttaatt tttctccagt tttcaattta ttaactgtgt
      241 atggcataac ctgtaattga tgatgatgtg ctttatcaac aaattttctg gccactaatg
      301 cataattagg attaacataa ctagcaattt gagcaattct tgaaaagttt ggctttttat
      361 accaataatt acgtttacta gaaagcacac ctaattcata tatactgccc aatgtgttta
      421 acttttcgat gcattcgata tcaaaagatt gtataactac ttgtgtcgca tcgactttct
      481 tttcttccaa aaatgccagc aatttacatt ctatttctgg atataaattc ggacttttca
      541 attcaatcaa tagcttttta tcatacttta agcataatga aagtacttca tctaacgtgg
      601 gtattcgttc tcccttaaaa gcaacatctt tataactacc aaaatcaaat gattttaatt
      661 gcgataatgt gtaatcagca atacgcccct taccatccga tgttctatca attgtttcat
      721 catgtatcac aacaaaatgt tggtctttgg tcaaatgaac atctatttct aacatagcaa
      781 cattgagccc cattacctct cgataaccga ccattgtatt ttcaggaaaa tcactcggta
      841 atccacggtg cgaaacaatt tgtaattcat ctttcagttt attcagagtc attgataagt
      901 cacctttctg ttaaactttg ataaactatt ctaaaattaa actatcacaa ttatattgga
      961 ggttaaagca attgcatcaa catgacttta aagtccaaac ttcttggcaa ggtggtcgta
     1021 acaatgtcgg aaacgttcaa ggcgacatac tttcagagaa tatttcaata cctgcttctt
     1081 taggtggtgt tggtatagga acaaatcccg atgaaatgtt agtatcagcc gcttcatcat
     1141 gttatatcat ctcattagca gctactcttg aacgtgcaaa gttcacagat atttcaattg
     1201 aacaacaatc gattggaaca gcttgtttaa ataatggaaa attcagtatg tcaaaaattg
     1261 tgcaccatcc tcacattgaa attccaagtg atcaaatagc acaattagaa aagcgattac
     1321 caaaattgat aacaattgca gataataatt gcatgatttc aaatgctgta agaaataatg
     1381 tagacataaa aatctatccc atcattcagg ccaaataaaa tcgttacgtg ataacgttta
     1441 ttcgacaata tagcgttatc acttttttta ggcttttaaa ttttcagaaa atttgctaaa
     1501 atagtgaaat cttatttata ggaggcatat aaatgtatta tcatcaaccg ttgttattaa
     1561 cacctggccc aacccctgta cctgatgcca ttatgagaga aattcaagca cctatggttg
     1621 gtcatcgttc taaagatttt gaagacatcg cacaacaagc atttcaaggt ctaaagccaa
     1681 tatttgggag tcaaaatgat gtacttattt taacatctag cggtacaagc gtcttggagg
     1741 ctagtatgtt gaacattgta aaccctgaag atcacttcgt tgtcattgtt tcaggtgcct
     1801 ttggtaaccg atttaaacaa attgcacaaa cttattacaa aaatgtgcac atttatgacg
     1861 taacatgggg agaagctgta gatgttaaag atttcatcaa ttacctttca actttagatg
     1921 ttgaagttaa agcagtattt agtcaatatt gcgaaacatc tacgacagtg ctacacccta
     1981 ttcacgagtt aggaaatgcc atcaatcaat ttaatagtaa tatttatttt gtagttgacg
     2041 gcgtaagttg cattggtgct gttgatgttg acattaacaa agataaaatt gatgtacttg
     2101 tttctggtag tcaaaaagca attatgttac ctccaggatt agcttttgta gcttatagcc
     2161 accgtgcaaa agaacgtttc aaagaagtaa ctacgccaaa attttatcta gacttaaata
     2221 aatacatttc gtcacaagct gacaattcta caccgttcac accaaatgtg tctttattta
     2281 gaggtgtaaa tgcatacgtt gaaaccgtaa aagcagaagg tttcaatcac gtaatagcac
     2341 gacactatgc aattagaaat gcattaagaa gcgccttaaa agcattagat ttaactttat
     2401 tagtcaatga taaagatgca tctccaacgg ttacagcatt caaacctaat acaaatgaag
     2461 tgaaaataat caaagatgaa cttaaaaatc gctttaaaat aacaattgct ggtggtcaag
     2521 gccatcttaa aggtcaaatt ttaagaattg gtcatatggg gaaaattagt cctttcgata
     2581 ttttatcggt agtatctgct ttagaaatta ttttaactga acaccgtaaa gttaactata
     2641 tcggtaaagg tatatcaaaa tatatggagg ttattcatga agcaatttaa tgtactcgtt
     2701 gcagatccca tatcaaaaga tggtatcaaa gcattattag atcacgaaca attcaatgta
     2761 gatattcaaa ctggcttgtc cgaagaagca ttaatcaaaa ttataccttc ataccatgct
     2821 ttaatcgttc gtagtcaaac tacggttact gaaaatatca taaatgctgc tgattcttta
     2881 aaagtaatcg cacgcgccgg tgttggtgta gataatatta atattaatgc tgcaacatta
     2941 aaaggtattt tagttattaa tgccccagat ggtaatacga tttcagctac tgaacattca
     3001 ctggcaatgt tattatcaat ggctcgaaat attccgcaag cacaccaatc acttacaaat
     3061 aaagaatgga atcgaaatgc atttaaaggt actgagcttt atcataaaac attaggtgtc
     3121 attggtgctg gtagaattgg tttaggtgtt gctaaacgtg cgcaaagttt cggaatgaaa
     3181 atactagctt ttgaccctta cttaacggat gaaaaagcaa aatctttaag tattacgaag
     3241 gcaacagttg atgagattgc ccaacattct gatttcgtta cattacatac accactaaca
     3301 cctaaaacaa aaggcttaat taatgctgac ttttttgcca aagcaaaacc tagtttgcaa
     3361 ataatcaatg tggcacgtgg tggtattatt gatgaaaagg cgctaataaa agcattagac
     3421 gaaggacaaa ttagtcgggc agctatcgat gtgtttgaac atgaacctgc aactgattcg
     3481 cctcttgttg cacatgataa aattattgtt acacctcatt tgggtgcatc aacagtcgaa
     3541 gctcaagaaa aagtggcaat ttctgtttca aatgaaatca tcgaaatttt aattgatggt
     3601 actgtaacgc atgcagtgaa tgcacctaaa atggacttaa gcaatataga tgatactgta
     3661 aaatcattca tcaatttaag ccaaacagtt ggtgaattag ctattcaatt aatgtacaat
     3721 gcaccaagct ctattaaaat tacgtacggt ggcgacttag cctctattga tagtagttta
     3781 ttaacacgta caattattac tcatatttta aaagatgatc ttggtcctga agtcaatatt
     3841 atcaatgctc taatgttgtt aaatcaacaa caagtgacat taaatattga aaataataaa
     3901 gcagagacag gttttagtaa ctacttagag gtagaactat caaacgatag cgattccgtt
     3961 aaagttggcg cttctgtctt tacaggtttc ggtccaagaa ttgttagaat taataatttt
     4021 tctgtagact taaagccaaa tcaatatcaa attgtgtcat atcataatga tactccaggt
     4081 atggtaggaa aaactggcgc attgttaggt aaatacaata tcaacattgc atctatgact
     4141 ttaggtagaa ctgaagcggg cggagatgcg ctaatgattt tatccgttga tcaacctgtt
     4201 tcaaacaata taattgatga acttaaacaa gttggtgaat acaatcaaat tttcacaact
     4261 gaattgacgg tacagtcata aacaatttat gtaaattcaa aatgccaaga agacattgga
     4321 ttacttccat tgcttcttgg cattttttca ttattaattt cattgtaact caatatgaaa
     4381 cctttggttc aatcattcac ttcaagttat gctgtcaaac aacaatcaaa ttacaaatta
     4441 tctagtacac ctctaagttc acctaaatga ttaataacat agtcggcatg atgcgcttct
     4501 aactcacctg cagcatcctt accttttaaa cctgttaatg ttccaataaa cgttgcacct
     4561 attttttgag cacttaataa gtcagctaac gaatcgccta ctataaatac gtcatcttta
     4621 tttacaatgt tatcttgctt attgatataa gattcatatt tatcgcgatt attaccatat
     4681 aaagctgcga tataactaaa aggattcggc tttcctaatg gtcgtgcttg cggatacata
     4741 ttctctgctt ctaaaacatc acttgctgtt gcaataaaat cagcttcaaa atatggtaac
     4801 aatcctaaat tttcaaatgg cacaacagtc tcagtataag gacgacctgt tgcaatacct
     4861 aattcgaaac cagcaccttt taaatcattc agaagtacct taacttcatc tactggtctc
     4921 aaaataattt cttgataaat ataacctgtc ttaaaagtag ttcgtgctat tttcttttca
     4981 acatcttcat acaacttcga tcctaaatac cattcttgat aaacttcttg cgctaacgtc
     5041 cataatgcac ccttcaaact aaataaagta gcatccgaaa catgtaactc tgttgttgca
     5101 aactcttcca aagcagcata aatattattt ttaccaactt ttacattatc caaaaattgt
     5161 aaaggtagtt gttcatttaa attaaaacag tctgctaagt ttgtgcttat attttgcaac
     5221 ttcaattcga caggctcatc ttgatacatg aatgcctcaa tttcatcatg tgataacttt
     5281 ttcaaaatat caattaaatg aatactaaaa acgataaata acatatccca atttgaattc
     5341 agccctagcg attttaattt gtttaaaatc ttatcttttt gaaaaattcg attcctaatg
     5401 tcttgtatat cgttatcagt caaagtttcc caatctatat gtgaatgaag acctaaataa
     5461 cacttatcca ttaataattc atataccgtt aatgcagaga catcgaaaca acgttcttca
     5521 cttaaaaaaa tgccatcaac atcaaataaa attttcttca caatccccac tccatttctg
     5581 aaaattcaga tataaatcat tctactattt gactaaaaaa agcgcaaacc ctattgaagt
     5641 tagatttgcg ctttagctgt ttaaatttta taaatgtttt tcaatttcat cagcaacctg
     5701 ctgtacgtgt gtaccgacaa taacttgagt tgaatgtttg ccattaacag taacaccaac
     5761 tgcaccggcg tttttaatct tctgttgatc aataatagat gtgtctttta actctagacg
     5821 caaccttgtt gcacaattgg ttaaattaac aatattctct tgaccgccta aaccttctaa
     5881 tatttgtata gcatgttgat gatatttact ttgtttaata tcattttcac caggagcaat
     5941 attatctttt acaactgttg gatcaactaa ttcattttca cctctaccaa tcgtattcaa
     6001 gttaaatact tggattacta cacggaaaat cacatagtat aagatgaaaa atacaacacc
     6061 ttgaacaagc aacatcaatg gatgatttga tactggatta attagtgata acacataatc
     6121 tatcaaacct gcactaaatg aaaatccagc tgtccaatgg aatgtagctg cgataaataa
     6181 agataatcct gttaataacg catgaacaac atataagatt ggcgcaacaa acataaatgc
     6241 aaactcaatc ggctctgtaa caccaacgaa aaatgctgca actgaactcg ctaggaacca
     6301 accgtaaact tgttttttct gagtcgtttt agctgtatga tacattgcta acgcagccgc
     6361 tggaataccg aacatcatga ttgggaagaa tcccgcttga tagcgtcctg taataccttt
     6421 tatagcatct ttgccacttt ggaatttacc aatatcatta ataccaatcg tatcaaacca
     6481 gaacacacta ttcagtgcat gatgtaatcc tgtaggaatt aataatctat tggcaacacc
     6541 atatatgaaa gctccaaacg atcctaaacc gactatagat tcaccaaatt ttacaatcca
     6601 tgaataaagt agtggccata agaataacaa tatgacaact aaaaatgtac agtaaaatgc
     6661 agtcataatt ggaactagac gtttaccact aaaaaatgat aatgctaatg gtaattctgt
     6721 ttcactaaac ttattgtatg cataggctgc tattaaacct attacaatac caacaaagac
     6781 attgccatta ttcatctttt caaaagctga atttatttcc gaagctttca ttcctaataa
     6841 aggcgctaat ttcattggtg ataatacaac tgtaactaaa aaatatccta acgtagctgc
     6901 aagcgcgact gcaccatcat ttttctttgc cattcctata gctacaccaa ttgcaaataa
     6961 aatacctaat tgctctaaaa tcgtagtacc taccgtagta aagaacattg cgattttcgg
     7021 cgtcgcatga agtgcattta acgtattacc aattccggca ataattgctg cagccggtaa
     7081 aatggcaact ggtaacatta acgaacgccc taaattttgg aaaaatttat acattgaatg
     7141 tcatccttct taaaataagg tagaaatata aagattacta atgtaactag aataactact
     7201 tcgatactcc gttatagtca cctaggcata ctaaccagct atatttctac ctcaagttat
     7261 tttataaact ttttacaatt tcatacaatt cttgttgtaa ctttgctgtt cgtgtttcaa
     7321 tctcttttgt aatgtaatcg atacgctcgt ttcgttttaa atctttaggt aaatcgttaa
     7381 tatcgattgg tttaccaata tttatgtatg cttgtcctgt taaaagacca tgaatcttag
     7441 taggaccaac ataagcaaca ggtaatattg gtgacttact taacattgca attgttgaag
     7501 caccacgttt caatggtgca ccttcttgcg atgtgcgaga acctgttggg aagataccaa
     7561 ctgtcttatt atctttcaac aaattgattg ggcgttttaa agtactaggt cctggatttt
     7621 cacgatctac aggaaatgca tttaaagacg ttaaaaattt accaatccat ttatttttga
     7681 ataattcttt tttagccata taatgaattt gattaggata taatgccata cctagcataa
     7741 taacttcgtt ataactttca tgcgtacaag ttacgacata tttactatcc ttaggaatat
     7801 tatctttacc gattacgtat aatgattttg acattttaac taaaatgaaa ttcaaaatct
     7861 tactaatcac tgaatacatt gtgccaccta cttaacttat ttgtttataa caactagatt
     7921 ttatcactat atttttacta gaacaagata gtctgaaaat acatctaaag ttgtatagtg
     7981 tattactagc aataattata aaaaaaagac ataattaaag catatataaa tcatataatg
     8041 gaggttaagt atgtcagatt ttaatcatac agatcattct acaacaaacc atagccaaac
     8101 acctagatac agaagaccta aatttccatg gtttaaaaca gtcatcgttg cattgattgc
     8161 tggaattatt ggtgcacttc tagtacttgg tataggcaaa gtattaaata gtacaatttt
     8221 aaataaagat ggttcaactg ttcagacaac aaataataaa ggtggcaatc aattagacgg
     8281 tcaaagcaag aaattcggta ccgttcatga aatgataaaa tctgtctccc ctacaattgt
     8341 tggagttatt aacatgcaaa aagcatcaag tgtagacgac ttattaaaag gcaaatcatc
     8401 taaaccatct gaagctggag taggttcagg tgttatctat caaataaaca acaattcagc
     8461 ttatatcgtt acaaacaatc atgttattga tggcgcaaat gaaattagag tccaattaca
     8521 taataaaaaa caagttaaag cgaaattagt tggtaaagat gcagtaactg atattgctgt
     8581 acttaaaatt gaaaatacaa aaggtattaa agcgattcaa tttgccaact cttcaaaagt
     8641 acaaactggc gatagcgtat tcgcaatggg taacccatta ggattacaat ttgctaactc
     8701 tgtaacatct ggtatcattt cagcaagcga acgtacgatt gacgctgaga caactggtgg
     8761 caatacaaaa gttagcgttc ttcaaacaga tgctgctatt aacccaggta actcaggtgg
     8821 cgcattagta gatattaatg gtaatttagt tggtattaac tcaatgaaaa ttgctgcgac
     8881 acaagttgaa ggtatcgggt ttgctattcc aagtaatgaa gttaaagtaa caattgaaca
     8941 acttgtaaaa catggtaaaa ttgaccgccc ttcgattggt attggtttaa ttaatttgaa
     9001 agatattcct gaagaagagc gcgagcaact tcatactgat agagaagacg gtatttatgt
     9061 cgccaaagct gatagtgata ttgatcttaa aaaaggtgat attattacag aaattgatgg
     9121 caagaaaatt aaagatgatg ttgatttaag aagctattta tatgaaaata aaaaacctgg
     9181 tgaatcagtc actgttaccg ttatccgtga tggtaaaaca aaagaagtta aagtgaaatt
     9241 aaaacaacaa aaagaacaac caaaacgtca aagccgatca gaacgtcaat cacctggcca
     9301 aggcgataga gatttcttta gataatttac agacagaaaa gtacattaag agcctactca
     9361 ttacattctt aggaatgaat atgagtaggc tctaatttat ttagctatgc aattatattt
     9421 attgatagtt aaccatgaag tattttttct taccgcgacg aataatcgta aattcgccat
     9481 caattttatc ttctggtgct aaagcataat taacatcttg ttgtctctca ccattaatat
     9541 aaatcgcacc attgttaaca tcttcacgtg cttgtcgttt agaaggagaa atgcctgttt
     9601 caataaggac ttcaacgata tttgttgtgt catttgataa tgtcacttga ggcacatctt
     9661 taaatccatc ttttaattct ttcgctgata atgattttaa atcaccacta aataatgctt
     9721 gtgaaatacg gattgcatca tttaatgcat cttcaccatg aataaattta gttacttctt
     9781 cagctaatgt tttttgagct tcacgtaaat gcggtgcttc atttttagat tgttctaagc
     9841 gatcaatttc ttcttttcct aagaaagtaa agtattttaa gaatttaatt acatcttcgt
     9901 ctgattgatt aatccagaat tgataaaatt cataaggact tgttttttca gcatctaacc
     9961 aaacagcacc tgactcagac ttaccaaatt tcttaccatc tgatttagtt acaagcggaa
    10021 tagttaaacc gtatgcgtct gtttgaccat acatacgacg cattaattca ataccacttg
    10081 tgatattacc ccattgatct gatccaccta cttgaatctt acaattcaat tctctattca
    10141 aatgaccgaa atcaatagct tgtaaaatcg tgtatgtgaa ttctgtatat gaaataccat
    10201 gttctaaacg actttggatt gaatctttac ctaacatgta attaacgccg acgtgtttac
    10261 catagtcacg taaaaagcta attaatgaga tttgtcctaa ccagtctcta ttattaacaa
    10321 gcactgcacc atggtctgtt ccaaattcaa aaatattgtg catttgctta ctaatacctt
    10381 cgatattttt atctacttgt tcttctgttt gtagcacacg ttcttctgat ttacctgatg
    10441 gatcaccaat catacctgta ccaccgccaa ttaaaacgat aggacgatgt ccatgttctt
    10501 gaaaacgtct taatgttaag aatggtagta agtgaccaat atgtaaacta tctgccgttg
    10561 gatcggcacc gcagtataac gtcacttgtt ctttatttaa taaatcttca ataccttgtt
    10621 catcagtttg ttgataaata agacctctcc attttaaatc ttcaattaat acattcgtca
//
LOCUS       DANBWH010000088        10523 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.091, whole genome shotgun sequence.
ACCESSION   DANBWH010000088 DANBWH010000000
VERSION     DANBWH010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10523
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.091"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            45..473
                     /locus_tag="PPY92_001521"
     CDS             45..473
                     /locus_tag="PPY92_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625527.1"
                     /translation="MTRSALKPFKNKRVMVTGRIQRVLFKNYLDRHSTFKPNVRILLK
                     DVFVSGVSIDHLWLYETNKYYALAMELIHQRVKFSANVVPYYKINRNNNLFVQDYGIK
                     RKGRLITEEAYNQNNQYQDKIYEKLPDIDFRLEDFYSKEN"
     gene            complement(682..1188)
                     /locus_tag="PPY92_001522"
     CDS             complement(682..1188)
                     /locus_tag="PPY92_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDJ2625528.1"
                     /translation="MAHIIRRVSIKDVENFISMLAKIYDESPYMFYTPGEYDPSVTSA
                     SKQLEEYITSPHKVIFVAESDEQLVGFAFVNTTPFQRIKHVAKIDLGVKKLYQHRGIG
                     QALLDAIMAWCLNNQIHRIEANVPLNNQPALELFKSADFQIEGVLKDKLFIDGKYYDD
                     YMMAKILN"
     gene            1301..2224
                     /gene="graX"
                     /locus_tag="PPY92_001523"
                     /gene_synonym="apsX"
     CDS             1301..2224
                     /gene="graX"
                     /locus_tag="PPY92_001523"
                     /gene_synonym="apsX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="auxiliary protein GraX/ApsX"
                     /protein_id="HDJ2625529.1"
                     /translation="MKPKVLLAGGTGYIGKYLSEVIENDAELFTISKYPDNKKTDDVE
                     MTWIQCDIFHYEQVVAAMNQIDIAVFFIDPTKNSAKITQSSARDLTLIAADNFGRAAA
                     INQVKKVIYIPGSRYDNETIERLGAYGTPVETTNLVFKRSLVNVELQVSKYDDVRSTM
                     KVVLPKGWTLKNVVNHFIAWMGYTKGTFVKTEKSHDQFKIYIKNKVRPLAVFKIEETA
                     DGIITLILLSGSLVKKYTVNQGKLEFRLIKESAVVYIHLYDYIPRLFWPIYYFIQAPM
                     QKMMIHGFEVDCRIKDFQSRLKSGENMKYTK"
     gene            2240..2914
                     /gene="graR"
                     /locus_tag="PPY92_001524"
                     /gene_synonym="apsR"
     CDS             2240..2914
                     /gene="graR"
                     /locus_tag="PPY92_001524"
                     /gene_synonym="apsR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor
                     GraR/ApsR"
                     /protein_id="HDJ2625530.1"
                     /translation="MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFN
                     PEIVILDVQLPKYDGFYWCRKMREVSNVPILFLSSRDNPMDQVMSMELGADDYMQKPF
                     YTNVLIAKLQAIYRRVYEFTAEEKRTLTWQDAVVDLSKDSIQKGDDTIFLSKTEMIIL
                     EILITKKNQIVSRDTIITALWDDEAFVSDNTLTVNVNRLRKKLSEISMDSAIETKVGK
                     GYMAHE"
     gene            2907..3947
                     /gene="graS"
                     /locus_tag="PPY92_001525"
                     /gene_synonym="apsS"
     CDS             2907..3947
                     /gene="graS"
                     /locus_tag="PPY92_001525"
                     /gene_synonym="apsS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase GraS/ApsS"
                     /protein_id="HDJ2625531.1"
                     /translation="MNNLKWVAYFLKSRMNWIFWILFLNLLMLGISLIDYDFPIDSLF
                     YIVSLNLSLTMIFLILTYFKEVKLYKHFDKDKEIEEIKHKDLAETPFQRHTVDYLYRQ
                     ISAHKEKVVEQQLQLNMHEQTITEFVHDIKTPVTAMKLLIDQEKNQERKQALLYEWSR
                     INSMLDTQLYITRLESQRKDMYFDYVSLKRMVIDEIQLTRHISQVKGIGFDVDFKVDD
                     YVYTDTKWCRMIIRQILSNALKYSENFNIEIGTELNDQHVSLYIKDYGRGISKKDMPR
                     IFERGFTSTANRNETTSSGMGLYLVNSVKDQLGIHLQVTSTVGKGTTVRLIFPLQNEI
                     VERMSEVTNLSF"
     gene            4091..4852
                     /gene="vraF"
                     /locus_tag="PPY92_001526"
     CDS             4091..4852
                     /gene="vraF"
                     /locus_tag="PPY92_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein VraF"
                     /protein_id="HDJ2625532.1"
                     /translation="MAILEVKQLTKIYGTKKMAQEVLRDINMSIEEGEFIAIMGPSGS
                     GKTTLLNVLSSIDYISQGSITLKGKKLEKLSNKELSDIRKHDIGFIFQEYNLLHTLTV
                     KENIMLPLTVQKLDKEHMLNRYEKVAEALNILDISDKYPSELSGGQRQRTSAARAFIT
                     LPSIIFADEPTGALDSKSTQDLLKRLTRMNEAFKSTIIMVTHDPVAASYANRVVMLKD
                     GQIFTELYQGDDDKHTFFKEIIRVQSVLGGVNYDL"
     gene            4842..6731
                     /gene="vraG"
                     /locus_tag="PPY92_001527"
     CDS             4842..6731
                     /gene="vraG"
                     /locus_tag="PPY92_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease VraG"
                     /protein_id="HDJ2625533.1"
                     /translation="MTFNEIIFKNFRQNLSHYAIYLFSLITSVVLYFSFVALKYAHKL
                     NMTESYPIIKEGSQVGSYFLFFIIIAFLLYANVLFIKRRSYELALYQTLGLSKFNIIY
                     ILMLEQLLIFIITAILGIIIGIFGSKLLLMIVFTLLGIKEKVPIIFSLRAVFETLMLI
                     GVAYFLTSAQNFILVFKQSISQMSKNNQVKETNHNKITFEEVVLGILGIVLITTGYYL
                     SLNIVQYYDSIGILMFILLSTVIGAYLFFKSSVSLVFKMVKKFRKGVISVNDVMFSSS
                     IMYRIKKNAFSLTVMAIISAITVSVLCFAAISRASLSSEIKYTAPHDVTIKDQQKANQ
                     LASELNNQKIPHFYNYKEVIHTKLYKDNLFDVKAKEPYNVTITSDKYIPNTDLKRGQA
                     DLFVAEGSIKDLVKHKKHGKAIIGTKKHHVNIKLRKDINKIYFMTDVDLGGPTFVLND
                     KDYQEIRKYTKAKHIVSQFGFDLKHKKDALALEKVKNKVDKSIKTRSEAISSISSLTG
                     ILLFVTSFLGITFLIAVCCIIYIKQIDETEDELENYSILRKLGFTQKDMARGLKFKIM
                     FNFGLPLVIALSHAYFTSLAYMKLMGTTNQIPVFIVMGLYICMYAVFAVTAYNHSKRT
                     IRHSI"
     gene            7454..8071
                     /locus_tag="PPY92_001528"
     CDS             7454..8071
                     /locus_tag="PPY92_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HDJ2625534.1"
                     /translation="MFSKKKDKFMVQLEEMVFNLDRAAIEFGKMDFNTHLDLKAYSDN
                     IKTYESHGDELVHQVITDLNQTFITPIEREDILSLCDAIDDVLDAIEETAAMFEMYSI
                     EYTDEYMAEFVDNIQKAVAEMKLAVGLLVDKKLSHMRIHSINIKEFETNCDGILRQSI
                     KHIFNSETDPITLIKIKDIYESMEEIADKCQIVANNFETIIMKNS"
     gene            8087..9094
                     /locus_tag="PPY92_001529"
     CDS             8087..9094
                     /locus_tag="PPY92_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HDJ2625535.1"
                     /translation="MSYIIIVTIAVVIFSLIFDFINGFHDTANAVATAVSTRALTPKT
                     AILMAAVMNFIGALTFTGVAGTITKDIVDPFKLENGLVVVLAAILAAIIWNLATWFYG
                     IPSSSSHALIGSIAGAAIASEGSFGVLHYQGFTKIIIVLIVSPIIAFCVGFLMYSIFK
                     VIFKNANLTRANRNFRFFQIFTAALQSFSHGTNDAQKSMGIITLALIVANVQNDGSVE
                     PQLWVKFACATAMGLGTAIGGWKIIKTVGGNIMKIRPANGAAADLSSALTIFVASSLH
                     FPLSTTHVVSSSILGVGASNRAKGVKWSTAQRMIITWVITLPISALLAGLLFYILNLF
                     F"
     gene            complement(9682..10479)
                     /locus_tag="PPY92_001530"
     CDS             complement(9682..10479)
                     /locus_tag="PPY92_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDJ2625536.1"
                     /translation="MKKLAFAITATSGAAAFLTHHDAQASTQHTVQSGESLWSIAQKY
                     NTSVESIKQNNQLDNNLVFPGQVISVGGSDAQNTSNTSPQAGSASSHTVQAGESLNII
                     ASRYGVSVDQLMAANNLRGYLIMPNQTLQIPNGGSGGTTPTATTGSNGNASSFNHQNL
                     YTAGQCTWYVFDRRAQAGSPISTYWSDAKYWAGNAANDGYQVNNTPSVGSIMQSTPGP
                     YGHVAYVERVNGDGSILISEMNYTYGPYNMNYRTIPASEVSSYAFIH"
BASE COUNT         3778 a         1370 c         1843 g         3532 t
ORIGIN      
        1 atagattgag cacgaagtga tttgaaataa ggttgtgaaa gggaatgaca aggtcagcat
       61 taaaaccatt taaaaataaa cgcgttatgg ttactggacg tatacaacgt gttttgttta
      121 aaaattattt agatagacat agcacattta agccgaatgt aaggatatta ttaaaagatg
      181 tatttgtttc aggtgtatca atagatcatt tatggttata tgagacaaat aaatactatg
      241 cattggcaat ggaacttatt catcaacgag taaaatttag tgcgaatgtt gtaccatatt
      301 acaaaataaa tagaaataat aatttattcg tacaagatta tggaattaag cgtaaaggta
      361 ggttaattac tgaagaagct tacaatcaaa acaatcagta tcaggataag atatatgaaa
      421 aattaccgga tatagatttt agactcgaag atttttatag taaggaaaac taaacaacat
      481 atataaagca ctactaatcg tagttgatag ttgatcaatt acacattagt ggtgctttat
      541 tttgaattta aatttgataa atgaataaga gtatacgcat tcatttaaga ttagatgaca
      601 ttgataagta tgagtaatgt attaggtaac cattaaattc gttgttatgc gattctgata
      661 caagattatg ataaaatagc tttaattaag aattttagcc atcatatagt catcgtaata
      721 tttaccatcg ataaataact tatcttttaa aacgccttcg atttgaaaat cggcactttt
      781 aaaaagctcg agggcaggtt ggttattgag tggtacattt gcttcaattc ggtgtatttg
      841 attgtttaaa caccaagcca taatggcatc aagaagtgct tggccaattc cacgatgttg
      901 atataatttc tttacaccta aatcaatttt agcaacatgt ttaatgcgtt gaaatggtgt
      961 cgtattaaca aaggcaaagc caacgagttg ttcatcactt tcagcaacaa agatgacttt
     1021 atgtggagaa gtgatatatt cttctaattg tttactagcc gacgtgacgc taggatcata
     1081 ttctcctggt gtgtagaaca tatacggaga ttcgtcgtat attttcgcta acattgaaat
     1141 gaaattttct acatctttga tactaactct acgtataata tgggccataa aaagcctcca
     1201 gtattttgaa attttaaaac atttgctact attataatat atatgtaact aaaaggtgga
     1261 gtaatatgtt ttttggttac agtttaaagg agtattttag atgaaaccta aagttttatt
     1321 agcaggtgga acaggatata ttggtaagta tttaagtgaa gtgattgaaa atgatgctga
     1381 actttttact atatcaaaat atccagacaa taaaaaaaca gatgatgttg aaatgacttg
     1441 gattcagtgt gatatatttc attacgaaca ggttgttgca gcaatgaatc aaatagatat
     1501 tgctgtattc tttatcgacc caacaaagaa ttctgccaaa ataacacaat catcagcaag
     1561 agatttaaca ttaatcgcag cagataattt tggtcgagca gcggctatca atcaagtaaa
     1621 aaaagtaatc tacatacctg ggagtcgtta tgataatgaa acaattgaac gcctaggtgc
     1681 atatggcaca cctgtagaaa caacaaattt agtttttaaa cgttctttag ttaatgtaga
     1741 attacaagtt tcaaagtatg atgatgttag atcaacgatg aaggtagttt taccaaaggg
     1801 atggacatta aagaacgttg taaaccattt tattgcatgg atgggttaca ctaaaggaac
     1861 ttttgtgaaa acagaaaaat cacatgatca atttaagata tatattaaga ataaggtgcg
     1921 accgctcgca gtatttaaaa tagaagaaac agctgacgga ataataactt taattttatt
     1981 gagtggaagt ttagtgaaaa aatacacagt taatcaaggg aagttagaat ttagattaat
     2041 caaagagtcg gcagtcgttt atatacatct atacgattat atccctcgat tattttggcc
     2101 gatttattac tttatacaag caccaatgca aaaaatgatg attcatggct ttgaagttga
     2161 ctgccggatt aaagattttc aaagtcgatt aaaatcagga gaaaatatga aatatactaa
     2221 atgatattgg gtgatatgga tgcaaatact actagtagaa gatgacaata ctttgtttca
     2281 agaattgaaa aaagaattag aacaatggga ttttaatgtt gctggtattg aagatttcgg
     2341 caaagtaatg gatacatttg aaagttttaa tcctgaaatt gttatattgg atgttcaatt
     2401 acctaaatat gatgggtttt attggtgcag aaaaatgaga gaagtttcca acgtaccaat
     2461 attattttta tcatctcgtg ataatccaat ggatcaagtg atgagtatgg aacttggcgc
     2521 agatgattat atgcaaaaac ctttctatac caatgtatta attgctaaat tacaagcgat
     2581 ttatcgtcgt gtctatgagt ttacagctga agaaaaacgt acattgactt ggcaagatgc
     2641 tgtcgttgat ctatcaaaag atagtataca aaaaggtgac gatacgattt ttctatccaa
     2701 aacagaaatg attatattag aaattcttat taccaaaaaa aatcaaatcg tttcgagaga
     2761 tacaattatc actgcattat gggatgatga agcatttgtt agtgataata cgttaacagt
     2821 aaatgtgaat cgtttacgaa aaaaattatc tgaaattagt atggatagtg caatcgaaac
     2881 aaaagtagga aaaggatata tggctcatga ataatttgaa atgggtagct tattttttga
     2941 aatctcgcat gaactggata ttttggatat tgtttttaaa cttgcttatg ttaggcatta
     3001 gtctaatcga ttatgatttt ccaatagaca gtttatttta tattgtttct ttgaatttaa
     3061 gtttaacaat gatttttctt atattgacat attttaaaga agtaaaatta tataagcatt
     3121 ttgacaaaga taaagaaata gaagaaatta aacataaaga tttagcggaa acgccatttc
     3181 aacgtcatac agttgattat ttatatcgtc aaatctcagc gcacaaagaa aaggttgttg
     3241 agcaacagtt acaattgaac atgcatgaac aaaccattac agaatttgtg cacgacataa
     3301 aaacacctgt gacagccatg aaattattaa ttgatcaaga aaaaaatcaa gaaagaaaac
     3361 aggcattact atatgaatgg tctcgtataa actcgatgct ggatacacag ctgtatatta
     3421 ctagattaga atctcaacgc aaagatatgt attttgatta cgtgtcactt aaacgcatgg
     3481 tcattgatga aatacaatta acaagacata ttagtcaggt taaaggtatt ggttttgatg
     3541 ttgactttaa agtggatgat tatgtttata cagatacaaa atggtgtcgt atgattatta
     3601 gacagatttt gtcaaacgca ttgaaatata gtgagaattt taatattgaa attgggacag
     3661 aattaaatga tcaacatgtt tcgttatata ttaaagacta tggcagaggt attagtaaaa
     3721 aagatatgcc gcgaatattt gaacgaggat ttacgtcaac ggctaacaga aatgaaacga
     3781 cgtcttcagg tatgggtcta tatttagtaa atagtgtaaa ggatcaatta ggtattcacc
     3841 tgcaagtcac gtcgactgtt ggtaagggga caactgtcag attgattttc ccattacaaa
     3901 atgaaattgt tgaacgcatg tcggaagtga caaatttgtc attttaaaca tgcgttttgt
     3961 tacttagaat tgatacatca atgcagcttc aacgttataa taagatagat gttagtcata
     4021 tgttaaattg aagatacaag tgccaaagcc taaaggaaat gaagttaaga taaattatag
     4081 gagtgttaaa gtggcaattt tagaagtaaa acaattaaca aaaatatatg gaactaaaaa
     4141 aatggcacaa gaagtgttgc gagatatcaa tatgtctatt gaagaaggcg agtttattgc
     4201 tattatgggt ccctctggat ctgggaaaac gacattatta aatgttttaa gttcaattga
     4261 ttatatttca caaggttcta ttacattaaa aggaaaaaaa ttagaaaagc tttcaaacaa
     4321 ggaattatct gatatacgca agcatgatat tggttttatt tttcaagagt ataatttact
     4381 gcatacattg actgttaaag aaaacataat gttaccacta acggttcaga agttagataa
     4441 agaacatatg ttaaatcgtt atgaaaaagt agcagaagca ttaaatatat tggatattag
     4501 tgataaatac ccttctgaat tgtctggtgg acaaagacaa cgaacatctg ctgcaagagc
     4561 gtttattaca ttaccttcta ttatatttgc tgacgaacca acaggtgcac tggattctaa
     4621 aagtactcaa gatttattaa aacgattaac aagaatgaat gaagcattta agtctacaat
     4681 tattatggta acgcatgatc ctgttgcagc aagttatgcc aatcgagtag tgatgctaaa
     4741 agatggtcaa attttcactg aattatacca aggggatgac gataaacata cctttttcaa
     4801 agaaataata cgtgtacaaa gtgttttagg tggcgttaat tatgaccttt aacgagataa
     4861 tatttaaaaa tttccgtcaa aatttatcac attatgccat ctatcttttt tcattaatta
     4921 cgagtgtagt attgtatttt agctttgtag cattaaaata cgcgcataaa ctaaacatga
     4981 cagagtcata tccaattatt aaggaaggct cacaagtcgg aagctacttt ctatttttca
     5041 tcataattgc atttttgtta tatgccaatg tgttatttat taaacgacga agttatgagc
     5101 ttgcattata tcaaacatta ggtttatcta aattcaacat tatttatata ctaatgctcg
     5161 aacaattact aatatttata attacggcaa tattaggtat tattattggt atttttggtt
     5221 caaaactgtt attaatgatt gtctttacat tattaggaat taaagaaaag gttccaatta
     5281 tttttagttt gagggcggta tttgaaacat taatgttaat cggtgtcgct tattttttaa
     5341 cctctgctca aaattttata ttagtgttca aacaatctat ttcacagatg tcaaagaata
     5401 accaggttaa agaaacaaat cataataaaa ttacatttga agaggttgtt ttaggcatct
     5461 taggtatagt attgattacc acaggatact atctatcttt gaacattgtt caatattatg
     5521 attctatcgg tatacttatg tttattttat tgtcaactgt gattggggca tacttatttt
     5581 ttaaaagctc tgtttctcta gtttttaaaa tggtgaagaa gtttagaaaa ggtgttataa
     5641 gtgtaaatga tgtcatgttc tcatcatcta ttatgtatcg tattaagaaa aatgcttttt
     5701 cacttacggt catggcaatc atttcagcga ttactgtttc agttctttgc tttgctgcta
     5761 taagtagagc gtccttatca agtgaaataa aatatactgc accacacgac gttacaatta
     5821 aagaccaaca aaaagctaat caattagcaa gtgaattaaa caatcaaaaa attcctcatt
     5881 tttataatta taaagaagta attcatacga aattgtataa agataattta tttgatgtaa
     5941 aagcgaaaga accatacaat gtaacaatta ctagtgataa atatatccct aatactgatt
     6001 tgaaacgtgg acaagctgat ttgtttgtag cggaaggttc tatcaaagat ttagtgaaac
     6061 ataagaagca tggtaaggca attataggaa cgaaaaaaca tcatgttaat attaagttac
     6121 ggaaagatat taataaaatc tattttatga cagatgttga tttaggtgga ccaacgtttg
     6181 tcttaaatga caaagactat caagaaataa gaaagtatac aaaagcaaag catatcgtct
     6241 ctcaatttgg attcgatttg aaacataaaa aagatgcttt agcattagaa aaagtgaaaa
     6301 ataaagttga taaatctatt aaaacaagaa gtgaagcgat aagctcaata tcaagtttaa
     6361 ccggaatatt attatttgta acatcatttt taggtattac attcttgatt gctgtatgtt
     6421 gcattatata cattaagcaa atagatgaaa ccgaagatga gttagagaat tatagtatat
     6481 tgagaaagct tggatttaca caaaaagata tggcaagggg actaaagttt aaaattatgt
     6541 ttaattttgg gttaccttta gttattgcac tatcacatgc atattttaca tcattagcat
     6601 atatgaaatt aatgggtaca acgaatcaaa taccggtttt catagtaatg ggattataca
     6661 tttgtatgta tgctgttttt gcagtgacgg cttataatca ttccaagcga acaattagac
     6721 attccatata aaatatacag atggctttca gtagagtagt ggattcgaat tcacgaacta
     6781 tactggaagc tttttattat aaatgaagag aagttatatt tttagcatgt atagttgaat
     6841 actgggttaa aataccatat taataatgaa gtaaaggtat gagtgattat gaaagtgttt
     6901 tgaatgaaat atatttaatt ggtgatgctt ttaattgaaa agattaacag gattcaactt
     6961 tgtaaattgt attaaatgtg agaaaataaa agtatattca ttgagatata tgagtcaatg
     7021 atcgttttaa acaagataag tgtattttaa tatgtaaaag ttatgtaata aatattgtat
     7081 cgttgcaaat ttcccaacta tatccattta caatttttag agttgtatag ataaaaaatt
     7141 gcctataaaa taactttttc aaaacaaatg cctactacta tttaaatttt atgtaaaagt
     7201 taagtttcgg aattgttaaa aatttgtaaa ttgaattgtg ggattgtaaa ttttgtgcta
     7261 ttttcatata agggcaaata cagtaaattg tttactgtga ggcatttttg aaattaatat
     7321 cagtacacta aaattatact gacgattgat aataacaaat ttgtatcatt agtttgtaaa
     7381 ttcacttgtc ttaatttgaa acaaattata actgaatgtg attggtgaca atcgcttaaa
     7441 tggaggattt taaatgttta gtaagaaaaa agataagttt atggttcaat tagaagagat
     7501 ggttttcaat ctggatcgtg ctgctattga attcggtaaa atggatttca atacacattt
     7561 agatttaaaa gcatactcag acaacattaa aacttatgag tcacatggtg acgaattagt
     7621 acatcaagta attactgatt taaatcaaac atttatcaca ccaattgaac gtgaagatat
     7681 tttatcatta tgtgatgcaa ttgatgatgt tttagatgca attgaagaaa cggcagctat
     7741 gtttgaaatg tattcaatcg aatacacaga tgaatatatg gctgagtttg ttgataacat
     7801 tcaaaaagca gttgcagaaa tgaaacttgc tgtcggctta ttagtcgata aaaaattatc
     7861 acatatgcgt attcattcaa ttaatattaa agaatttgaa acaaactgtg atggtatttt
     7921 aagacagtca attaaacata ttttcaatag cgaaacagat ccaatcacat taattaaaat
     7981 aaaagatatt tatgaaagca tggaagaaat tgctgataaa tgtcaaatcg tagcaaataa
     8041 ttttgaaact attattatga aaaatagcta aggggagtat atatttatgt catatataat
     8101 catcgtcact atagctgtag ttattttctc gctgatattt gactttatca atggattcca
     8161 tgatacagcc aatgcagtag ctactgctgt atctactaga gcgttaacgc ctaaaacggc
     8221 aattttaatg gcagcagtga tgaactttat aggtgcttta acatttacgg gcgttgcagg
     8281 caccattact aaagacattg tcgatccatt taaattggaa aatggattag ttgttgtgtt
     8341 agctgcaata cttgcggcta ttatttggaa tttagctact tggttttacg gaattccaag
     8401 ttcgtcttca catgcactta taggttcaat tgcgggtgca gcaatcgcat ctgaaggctc
     8461 atttggagtg ttacattacc aaggtttcac aaaaattatt attgtattaa tcgtttcacc
     8521 gataatcgca ttttgtgttg gtttcttgat gtattcaatt tttaaagtta tctttaaaaa
     8581 tgcaaattta acaagagcga atcgtaactt tagatttttc caaattttca cggcagcgtt
     8641 acaatcattc tctcacggta cgaatgatgc gcaaaaatca atgggtatta ttacgttggc
     8701 attgattgtc gctaatgtac agaatgatgg cagtgttgaa ccacagttat gggtaaaatt
     8761 tgcctgtgcg acagcaatgg ggcttggtac tgcaattggt ggctggaaaa ttatcaaaac
     8821 tgtaggtggt aatattatga aaatacgtcc agcaaatggt gctgcggccg atttatcatc
     8881 tgcattaaca atttttgttg catcatcgct acatttccca ttatcaacaa ctcacgttgt
     8941 gtcatcatca atcttaggtg ttggtgcttc taaccgagct aaaggtgtaa aatggagcac
     9001 tgcgcaacga atgatcatta catgggtgat tacattacct atttcagcat tgttagcagg
     9061 tttactattc tatatactta acttattttt ctaattgaaa ataaaactaa actgaacttc
     9121 agtatcacaa acatatggtg gtattgaagt tcattttttt atgtaagtag ataacatatt
     9181 ttatagaatt tacgtagaat gaccaagaca aagcatttta attagggggc taagttataa
     9241 atttattcca tggaacataa acagttaggt cattaaaata taagtaagcg taaaataatg
     9301 atttaatagt ttatattctt caatgaagga ataattaaat agttatcaac ttgtttaatt
     9361 aacctaaatt gtattgggct ttatttaata taaatttgta tatgtagaag aaaaagcgta
     9421 aaatactggt ataacaaatt aaatcattat atagtcaact tagttgcatt attttgattt
     9481 ttaatatatt atgcatttaa tttaatcaaa taggtaggtt attatttttt acctaactac
     9541 cattcataca aaatataaat aagtatgata gggaagggtg agataatttt ttgtaccaaa
     9601 atgaaaattt ttttcaaaaa taaaaaacta gcaatctcac atgatgtgaa ttgctagtat
     9661 atatcagtac aatttattta attaatggat gaatgcatag ctagaaactt ctgaagctgg
     9721 aattgtacgg tagttcatat tgtatggacc atatgtgtaa ttcatttcag aaatcaagat
     9781 actaccatca ccattgacac gttcaacata agcaacatga ccatatggac caggtgtgct
     9841 ttgcataatt gaaccaactg atggtgtgtt gtttacttgg taaccatcat tagctgcgtt
     9901 accagcccaa tacttagcgt ctgaccaata tgtgctaatt ggactaccag cttgagcacg
     9961 acggtcaaat acgtaccatg tacattgacc agcagtgtat aaattttggt gattaaaaga
    10021 tgatgcattg ccattgctac ctgttgtagc tgttggtgtt gtaccacctg atccaccatt
    10081 aggaatttgt aatgtttggt taggcataat taaataacca cgtaagttat tggctgccat
    10141 taattgatca actgaaacac catatctgct agcaatgata tttaatgatt caccagcttg
    10201 tacagtatga gatgatgctg aaccagcttg tggagaagtg tttgacgtat tttgtgcatc
    10261 acttccacct actgagataa cttgaccagg gaataccaag ttgttatcta attggttatt
    10321 ttgtttaata ctctctactg aagtgttgta tttttgagca atactccata atgattcacc
    10381 agattgtact gtatgttgtg tagaagcttg tgcatcatga tgcgttaaaa atgcagctgc
    10441 accagatgtt gctgttattg caaatgctaa ttttttcaaa gggactcctc cttaaaatta
    10501 agttttacta cttttaaaaa tta
//
LOCUS       DANBWH010000089        10371 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.092, whole genome shotgun sequence.
ACCESSION   DANBWH010000089 DANBWH010000000
VERSION     DANBWH010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10371
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.092"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            26..1204
                     /gene="deoB"
                     /locus_tag="PPY92_001531"
     CDS             26..1204
                     /gene="deoB"
                     /locus_tag="PPY92_001531"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDJ2625537.1"
                     /translation="MTRPFNRVHLIVMDSVGIGEAPDAADFKDEGSHTLRHTLEGFDQ
                     TLPNLEKLGLGNIDKLPVVNAVEQPEAYYTKLSEASVGKDTMTGHWEIMGLNIMQPFK
                     VYPNGFPEELIQQIEEMTGRKVVANKPASGTQIIDEWGEHQMKTGDLIVYTSADPVLQ
                     IAAHEDIIPLEELYDICEKVRELTKDPKYLIGRIIARPYVGEPGNFTRTSNRHDYALK
                     PFGKTVLDHLKDGGYDVIAIGKINDIYDGEGVTEAVRTKSNMDGMDQLMKIVKKDFTG
                     ISFLNLVDFDALYGHRRDKPGYAQAIKDFDDRLPELFSNLKEDDLVIITADHGNDPTA
                     PGTDHTREYIPVIMYSPKFKGGHALESDTTFSSIGATIADNFNVTLPEFGKSYLKELK
                     "
     gene            complement(1333..2148)
                     /gene="phnE"
                     /locus_tag="PPY92_001532"
     CDS             complement(1333..2148)
                     /gene="phnE"
                     /locus_tag="PPY92_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDJ2625538.1"
                     /translation="MTQEIAKYNVHTKAHKRKLIKRWLIAIVVLAIIIWAFAGVPSLE
                     LKSKSLEILKSIFSGLFHPDISYIYIPDGEDLLRGLLETFAIAVVGTFIAAIICIPLA
                     FLGANNMVKLRPVSGVSKFILSVIRVFPEIVMALIFIKAVGPGSFSGVLALGIHSVGM
                     LGKLLAEDIEGLDFSAVESLKASGANKIKTLVFAVIPQIMPAFLSLILYRFELNLRSA
                     SILGLIGAGGIGTPLIFAIQTRSWDRVGIILIGLVLMVAIVDLISGSIRKRIV"
     gene            complement(2145..2945)
                     /gene="phnE"
                     /locus_tag="PPY92_001533"
     CDS             complement(2145..2945)
                     /gene="phnE"
                     /locus_tag="PPY92_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDJ2625539.1"
                     /translation="MPLEIPTKYDSLLKKKVSLKTSFTFMLIIVLIIWSFIYTGFNFG
                     DLMIGIPQIGDLFKQMIPPDFEYLQQITTPMLDTIRMAIVSTVLGSIVSIPIALLCAS
                     NIVHQKWISIPSRFILNIVRTIPDLLLAAIFVAVFGIGQIPGILALFILTICIIGKLL
                     YESLETIDPGPMEAMTAVGANKIKWIVFGVVPQAISSFMSYVLYAFEVNIRASAVLGL
                     VGAGGIGLFYDQTLGLFQYPKTATIILFTLVIVVVIDYISTKVRAHLA"
     gene            complement(2947..3720)
                     /gene="phnC"
                     /locus_tag="PPY92_001534"
     CDS             complement(2947..3720)
                     /gene="phnC"
                     /locus_tag="PPY92_001534"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HDJ2625540.1"
                     /translation="MSQIEFKNVSKVYPNGHVGLKNINLNIEKGEFAVIVGLSGAGKS
                     TLLRSVNRLHDITSGEIFIQGKSITKAHGKALLEMRRNIGMIFQHFNLVKRSSVLRNV
                     LSGRVGYHPTWKMVLGLFPKEDKIKAMDALERVNILDKYNQRSDELSGGQQQRISIAR
                     ALCQESEIILADEPVASLDPLTTKQVMDDLRKINQELGITILINLHFVDLAKEYGTRI
                     IGLRDGEVVYDGPASEATDDVFSEIYGRTIKEDEKLGVN"
     gene            complement(3915..4871)
                     /locus_tag="PPY92_001535"
     CDS             complement(3915..4871)
                     /locus_tag="PPY92_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDJ2625541.1"
                     /translation="MKNFKYLFVLMLAVIVFAAACGNSSSLDNQKNASNDSDSKSGGY
                     KPKELTVQFVPSQNAGTLEAKAKPLEKLLSKELGIPVKVSVSTNYNTIVEAMKSKKVD
                     VGFLPPTAYTLAHDQKAADLLLQAQRFGVKEDGSASKELVDSYKSEILVKKDSKIKSL
                     KDLKGKKIALQDVTSTAGYTFPLAMLKNEAGINATKDMKIVNVKGHDQAVISLLNGDV
                     DAAAVFNDARNTVKKDQPNVFKDTRILKLTQAIPNDTISVRPDMDKDFQEKLKKAFID
                     IAKSKEGHKIISEVYSHEGYTETKDSNFDIVREYEKLVKDMK"
     gene            5100..6644
                     /locus_tag="PPY92_001536"
     CDS             5100..6644
                     /locus_tag="PPY92_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDJ2625542.1"
                     /translation="MKKIYKSLTVSAIVATVSLSALPQSLAITHESQPTKQQRTVLFD
                     RSHGQTAGAADWVSDGAFSDYADSIQKQGYDVKAIDGHSNITEASLKSSKIFVIPEAN
                     IPFKESEQAAIVNYVKQGGNVVFISDHYNADRNLNRIDSSEAMNGYRRGAYEDMSKGM
                     NAEEKSSTAMQGVKSSGWLSTNFGVRFRYNALGDLNTSNIVSSKESFGITEGVKSVSM
                     HAGSTLAITNPEKAKGIVYTPEQLPAKSKWSHAVDQGIYNGGGKAEGPYVAISKVGKG
                     KAAFIGDSSLVEDSSPKYVREDNGEKKKTYDGFKEQDNGKLLNNITAWMSKDSDGKSL
                     KASGLTLDTKTKLLDFERPERSTEPEKEPWSQPPSGYKWYDPTTFKAGSYGSEKGADP
                     QPNTPDDHTPPNQNVKISFDIPQNVSVNEPFEVTIHLKGFEANQTLENLRVGIYKEGG
                     RQIGQFSSKDNDYNPPGYSTLPTVKADENGNATIKINAKVLESMEGSKIRLKLGDKTL
                     ITTDFK"
     gene            6695..8230
                     /locus_tag="PPY92_001537"
     CDS             6695..8230
                     /locus_tag="PPY92_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDJ2625543.1"
                     /translation="MSNIAFYVVSDVHGYIFPTDFTSRNQYQPMGLLLANHVIEQDRR
                     QYDQSFKIDNGDFLQGSPFCNYLIAHSGSSQPLVDFYNQMAFDFGTLGNHEFNYGLPY
                     LKDTLRRLNYPVLCANIYENDSTLTDNGVQYFQVGDQTVGVIGLTTQFIPHWEQPEHI
                     QSLTFHSAFETLQQHLPEMKRHADIIVVCYHGGFEKDLENGTPTEVLTGENEGYAMLE
                     AFSKDIDIFITGHQHRQIAERFKQTAVIQPGTRGTTVGKVVLSTDEYENVSVESCELL
                     PVIDDSTFTIDEDDQHLRKQLEDWLDYEITTLPYDMTINHAFEARVAPHPFTNFMNYA
                     LLEKSDADVACTALFDSASGFKQVVTMRDVINNYPFPNTFKVLAVSGAKLKEAIERSA
                     EYFDVQNDEVSASADFLGPKPQHFNYDIYGGVSYTIHVGRPKGQRVSNMMIQGHAVDL
                     KQTYTICVNNYRAVGGGQYDMFIDAPVVKDIQVEGAQLLIDFLSNNNLMRIPQVVDFK
                     VEK"
     gene            8387..9154
                     /locus_tag="PPY92_001538"
     CDS             8387..9154
                     /locus_tag="PPY92_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625544.1"
                     /translation="METIKQEVRESIVKGIIAGYKDYVNERNEVKKRMVISDAYAFTK
                     SNHIESQVAKHLENFVKYIKENAGPSWKYLKFIFNKDEEKNNIMFILKNEDYFDEKNV
                     SVGKSLVADKNPKSKNYLEVLMAKNRDINFGTVGEDFEIGHQMTADSILFNIKEGSNR
                     DINSYFLIITYRIDKESKQLAAIKQWLPNPETNSAIMVDDLTELIEKVIVEREDYHID
                     EEELEVLKNDGELELIDVEHAFGISIDEGNDMIESER"
     gene            9160..10335
                     /locus_tag="PPY92_001539"
     CDS             9160..10335
                     /locus_tag="PPY92_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDJ2625545.1"
                     /translation="MFIGKNLEYVRKLNALSRKELSEKINVSEQAIWQYETKNMMPEI
                     SKIYDMTSIFNVKSSYFISEQPEELLINSVDKHSIAFRAKNYKVSTKLLNKQYYQAMY
                     LSNLTSYLFSFVTIPDNIILSLINNLDELLNGNLESLNKKESIKEIAKVVRAKILQDE
                     SNEALLFMLEKAGIVIYEKRINDSIDAYSFWSKDQTPFIILGTNRGVAVRRNFDLAHE
                     LGHLVLHRHIQFDLLSPEEYKTIEHEADIFASEFLLPEEAFKKDFDQMTKKSNPDYLA
                     VLKEKWYVSIQAIAMRAYYLGLMSSTQYRYFWASLNKKGYKSKEPLDDVIEMSRPVKM
                     NSLLKLYFDRNILTPQKLLNYLKVDETFLNHLAGINLKLFKDYVNENREYNITNLYK"
BASE COUNT         3596 a         1609 c         1892 g         3274 t
ORIGIN      
        1 tatataaaat ttgggagtga tagctatgac aagaccattt aatcgtgtac atttaatcgt
       61 aatggattca gtaggtattg gtgaagcgcc agacgcagct gattttaaag atgaaggttc
      121 acatacttta agacatacct tagaaggttt cgatcaaact ttaccaaacc ttgaaaagtt
      181 aggtctaggg aacatcgata aattaccagt agtaaatgca gttgaacaac cagaagcata
      241 ctatactaaa ttgagtgaag cttcagttgg taaagataca atgactggtc actgggaaat
      301 tatgggatta aatattatgc aaccttttaa agtataccct aatggattcc ctgaagagtt
      361 aattcaacaa attgaagaaa tgacaggtcg taaagttgtt gctaacaaac cggcatcggg
      421 tacgcaaatt atcgatgagt ggggcgagca ccaaatgaaa actggtgact taattgttta
      481 tacaagtgca gacccagtat tgcaaattgc tgcacatgaa gacattatcc cattagaaga
      541 gttatatgat atttgtgaaa aggttcgtga gttgacaaaa gaccctaaat atttaattgg
      601 tcgtattatc gcacgtccat atgttggtga accaggaaac tttacacgta catctaatcg
      661 acatgactat gcgttaaaac catttggtaa aactgtctta gatcatttga aagacggtgg
      721 ttatgatgtt attgccatcg gtaaaattaa tgacatttat gatggtgaag gtgtaacaga
      781 agcggttcgt acgaagagta acatggacgg tatggatcaa ttgatgaaaa ttgttaagaa
      841 agatttcaca ggtattagct tcttaaactt agtagacttt gatgcattat acggtcatcg
      901 tcgtgataaa ccaggttatg cacaagcaat taaagatttc gatgatcgct tgccagaact
      961 gtttagcaac ttaaaagaag acgatttagt aattattaca gcagaccatg gtaatgaccc
     1021 gacagcgcca ggtacggacc atacgagaga atatatccca gtaattatgt acagtccgaa
     1081 atttaaaggt ggtcatgcac tagaaagtga tactacattc agttctatcg gtgcaactat
     1141 agcagataat ttcaacgtaa cattaccaga gttcggtaaa agttatttaa aggaattgaa
     1201 atagaataaa tttagatatt ataaaaacag cagtgaagtt aactataaca atagttttct
     1261 tcactgctgt ttttattata atagagaaac gtgacggtag gacctcttat ttaggagtat
     1321 cctgatttaa tgttaaacaa tacgttttcg gattgaaccg gaaattaaat cgacaattgc
     1381 gaccattagt actaaaccga ttaatataat acctacacgg tcccaagaac gtgtttgaat
     1441 ggcaaatatg agtggtgtcc cgataccacc agccccaatt agccccagta tagaagctga
     1501 acgtaagttt agttcaaagc gataaagtat gagtgataga aaggcaggca taatttgtgg
     1561 tatgactgca aatacgagtg ttttaatctt attcgcacca ctggccttta atgattctac
     1621 agcactgaaa tctagacctt caatatcttc agctaaaagt ttcccaagca tacctacaga
     1681 atggatacct aaagctaata cacctgaaaa tgaacctggg ccaacagctt tgataaatat
     1741 aagtgccatt acaatttctg ggaagacacg tataacactt aaaataaatt tgctaacacc
     1801 tgaaaccggg cgtaacttta ccatattatt tgcacctaaa aatgctaatg gaatacagat
     1861 aattgcggcg atgaaagtac ctacaacggc tatcgcaaag gtttcaagta aaccacgtaa
     1921 taagtcttcg ccatctggta tatagatata gctgatgtca ggatggaata atccgctgaa
     1981 tatggatttt aagatttcta atgatttact tttaagttct aaacttggta cacctgcaaa
     2041 tgcccagatg ataatagcta agacgacaat tgcaataagc catcttttaa tcaattttcg
     2101 tttgtgtgct tttgtgtgaa cattatattt tgctatttcc tgtgtcatgc gagatgtgcc
     2161 ctcactttcg tactgatgta atcaatgacg acgacgataa ctaaagtaaa taaaataatc
     2221 gttgctgttt ttggatattg aaataaacca agtgtttgat cataaaacaa tccaataccg
     2281 ccagcgccga ctaatccaag cacagctgaa gctcgtatat ttacttcaaa tgcatataat
     2341 acgtatgaca taaatgacga tatggcttgt ggtacaacac cgaaaacaat ccattttatt
     2401 ttattagcgc caacagccgt cattgcttcc attggacctg gatctatcgt ttccaatgat
     2461 tcatataata attttccaat aatacagata gttaaaataa acaatgctaa tatccctgga
     2521 atttgaccga ttccaaatac agccacaaag attgctgcta ataacaaatc tggaatagta
     2581 cgaactatat ttaaaataaa gcgcgagggt attgaaatcc acttttgatg aacgatattg
     2641 ctagcacata ataacgcaat tggtattgaa acgatgctac ctaatactgt acttacgata
     2701 gccattcgaa tggtatctaa cattggcgtt gtaatttgtt gtaaatactc gaaatcaggt
     2761 ggaatcattt gtttgaatag atcacctatt tgaggtattc ctatcattaa atctccaaaa
     2821 ttaaaccccg tataaatgaa gctccaaatg ataagcacaa tgattaacat gaaggtaaaa
     2881 ctcgttttta aagaaacctt tttctttaaa agggagtcat actttgtagg tatttctaaa
     2941 ggcatgttag ttcactccta gcttttcatc ttctttaatt gtacgtccat atatttcact
     3001 aaatacgtca tctgttgctt cagatgcagg accatcatag acaacttcac catcacgtaa
     3061 accaatgatg cgtgtgccat attcttttgc caagtcaaca aaatgtaaat taattaaaat
     3121 tgtgatgcct aattcttggt tgatttttct taaatcatcc ataacctgtt tcgttgttaa
     3181 tgggtctaat gaagcaactg gttcatctgc aagaataatt tcggattctt ggcatagcgc
     3241 acgtgcaata gatatacgtt gttgttggcc acctgataat tcatcagagc gttgattata
     3301 tttatctaag atattgacgc gttctagtgc atccattgct ttaattttgt cttcttttgg
     3361 gaataaacct aataccattt tccaagtagg gtgataacct acacgtccac ttagtacatt
     3421 tcgtaataca cttgaccgtt taactaaatt aaaatgttgg aaaatcatac ctatatttcg
     3481 gcgcatttct aataatgctt taccatgggc tttagtgatg gatttgcctt ggatgaaaat
     3541 ttcacctgac gtgatatcat gcaaacgatt tacagatctt aataacgtgg atttcccagc
     3601 accagatagt ccgacaataa ctgcaaattc acctttttca atatttaagt taatattttt
     3661 caagcctaca tgaccgttag gatagacttt actgacgttt ttaaattcga tttgactcat
     3721 gtttgacacc tttctttaat aaagaaaagc caaggtaaac tgaaatatca tagacattgg
     3781 gtttctgtgt acaaatggtt tcaaatgtac agcgacacct ttctatatct gtttcagttt
     3841 agcgcctagc ttttcaatat ttatctagaa tatatctatt gaacgaaagc ttttaatacc
     3901 aaattcgata atgattattt catatcttta actaattttt cgtactctct tacaatgtcg
     3961 aaatttgaat ctttcgtttc tgtgtatcct tcatgtgaat aaacttcgct aataattttg
     4021 tgaccttctt ttgatttagc aatgtctata aaagcttttt tcaatttttc ttggaaatct
     4081 ttatccatat ctggtcttac agaaattgtg tcattcggaa tagcttgtgt taattttaaa
     4141 attcgtgtgt ctttaaatac atttggttgg tcttttttca cagtattacg tgcatcgtta
     4201 aatacagccg cagcatctac atctccattt aataatgaga taactgcttg gtcatgacct
     4261 ttaacattca caattttcat atctttagtt gcattaatac ctgcttcgtt ttttaacatc
     4321 gcaagtggga atgtatatcc agcagttgat gttacatctt gtaaggcaat tttcttacct
     4381 tttaaatctt tcaagctttt aatttttgag tcttttttaa caagaatttc tgatttataa
     4441 ctatctacaa gttctttact tgctgaacca tcttctttta caccaaaacg ttgtgcttgt
     4501 aataataaat cagctgcttt ttgatcatgt gctaatgtgt atgccgttgg tggtaagaaa
     4561 ccaacatcaa cttttttaga cttcatagct tcaacaattg tattgtagtt agttgataca
     4621 gacactttaa ctggaattcc taattcttta gatagtaatt tttctaatgg ttttgcttta
     4681 gcctctaatg ttccagcatt ttgcgaaggt acaaattgta ctgttaattc tttaggtttg
     4741 tatcctcctg atttagaatc cgaatcatta ctagcgttct tttgattatc taaagaactt
     4801 gagtttccac atgctgctgc aaaaacaatg actgctaaca ttaatacaaa taaatactta
     4861 aaatttttca tttgataact gtccccttca cattcttaat cgtgtaatat gtatgcaatg
     4921 aaattacact tatagcgtac cacatttaaa tgtgaaaaag tattttataa aaatgaattt
     4981 taataacttt ctttaagagt ttgataagat tttgtaaact tttcgttaaa ttgctcatat
     5041 gcaacgaatt tttatcctat aattcaagaa caaatttatt ttaaaaggag cctcacaaaa
     5101 tgaaaaaaat atataagtca ttaactgtct ctgcaattgt tgcaacggta tcattaagtg
     5161 ctttaccgca atctttagct ataacgcatg aatcgcaacc tacaaagcaa cagcgaacgg
     5221 tattattcga tcgttctcat ggtcaaacag ctggtgctgc agattgggtt agtgatggtg
     5281 cattttcaga ttatgcggat tcaatacaaa aacaaggtta tgacgttaaa gctattgatg
     5341 gtcattcgaa cataacagaa gcaagtttga aaagttccaa aatatttgta attcctgagg
     5401 ctaacattcc tttcaaagaa tcagaacagg cagcaattgt taactatgtg aaacaaggtg
     5461 gcaatgttgt ctttatttca gatcattaca atgctgaccg aaatttaaat cgtattgatt
     5521 catcggaggc aatgaatggt tatcgacgtg gagcatatga agatatgtcg aaaggtatga
     5581 atgcagaaga aaaaagctct actgcaatgc aaggtgtgaa aagttcaggt tggttatcta
     5641 caaactttgg cgtacgtttt cgatataatg cactaggtga tttaaatacg agcaatattg
     5701 tttcttcaaa agaaagtttc ggtattactg aaggtgtgaa atctgtctct atgcatgccg
     5761 gatcgacatt agcaattact aatccagaga aagcaaaagg tattgtgtat acaccagaac
     5821 aattgccagc gaaaagtaaa tggtcacatg ctgtagatca aggtatttat aatggtggcg
     5881 gtaaagcaga aggcccctat gtagcaattt ctaaagttgg aaaaggtaaa gcagcattta
     5941 tcggtgattc atcacttgtg gaagatagtt cgcccaaata tgtaagagaa gataatggag
     6001 aaaagaagaa aacatatgat ggttttaaag aacaagacaa cggtaagcta ttaaataata
     6061 taacggcttg gatgtctaaa gatagtgatg ggaaatcact taaggcgagt ggactaacat
     6121 tagatacaaa gactaagttg cttgattttg aacgaccaga gcgttcaact gagcctgaaa
     6181 aagagccatg gtcacaaccg ccgagtggtt ataaatggta tgacccaaca acatttaaag
     6241 caggtagtta tggcagtgaa aaaggcgcgg atcctcagcc aaacacacca gatgatcata
     6301 cgccaccaaa tcagaacgta aaaatatcat ttgatatccc gcaaaatgtt tctgtaaatg
     6361 agccatttga agtgacaata catttaaaag gatttgaagc aaatcaaaca cttgaaaatc
     6421 ttagagttgg tatttacaaa gaaggcggac gtcaaatcgg acaattttca agtaaagata
     6481 acgattataa cccaccaggt tacagtactt tgccaacagt taaagcagat gaaaacggaa
     6541 atgctacaat taagatcaat gctaaagtac ttgaaagtat ggaaggttca aagattcgtt
     6601 taaaactcgg tgacaaaacc ttgattacaa cagacttcaa ataaatatat aatagaaata
     6661 agaaagatgt ttgtgattga aggagtgagt gaggatgtca aacatagcat tttatgtcgt
     6721 gagcgacgta catggttata ttttcccaac agattttacg agtagaaatc aatatcaacc
     6781 tatgggattg ttactagcga atcatgttat agaacaagac agaaggcagt atgaccaaag
     6841 ttttaaaata gataatggtg attttttgca agggtcacca ttttgtaatt acttaatcgc
     6901 gcatagcggc agtagccagc ctttagttga tttttataat caaatggcat tcgactttgg
     6961 tacgcttggt aatcatgaat ttaattatgg attgccatac ttaaaagaca ctttacgcag
     7021 actcaattat ccagttttgt gcgctaatat ttatgaaaat gatagtacat tgactgataa
     7081 cggtgtgcag tattttcagg ttggagatca aacggttggt gtgataggtt taacgacaca
     7141 atttattccc cattgggaac aaccagagca tattcagtca cttacgtttc atagtgcttt
     7201 tgaaacactt caacaacact tacctgaaat gaagcgacat gcagatatca ttgtggtttg
     7261 ttaccatggt ggatttgaaa aggatttaga aaatggtacg ccgaccgaag tattaacggg
     7321 tgaaaatgaa ggatatgcca tgttagaagc gttttctaaa gatatagata tctttattac
     7381 gggtcaccaa catcgacaaa ttgctgaaag atttaagcaa acggctgtga ttcaacctgg
     7441 tacgagaggt acaactgtag gcaaagttgt cttgagtact gatgaatatg aaaatgtatc
     7501 cgttgaatca tgtgaattac ttcctgttat agatgattcc acatttacta ttgatgaaga
     7561 tgaccaacat ttacgaaagc agttagagga ctggttagat tacgaaatta ctacattgcc
     7621 atatgatatg acgattaatc atgcatttga ggcacgtgtg gcaccgcatc cttttactaa
     7681 ttttatgaat tacgctttat tagaaaaaag tgacgcagat gttgcctgta cagctttgtt
     7741 tgattctgct agtggtttca agcaagtcgt gacgatgcga gatgttatta acaattaccc
     7801 atttccaaat acatttaaag ttttagctgt aagtggtgcc aaacttaaag aagccattga
     7861 acgatcagca gaatattttg acgtgcaaaa tgatgaagta agtgcgagcg cagacttcct
     7921 tggacccaaa ccacaacact ttaattatga tatatatggt ggcgtaagtt ataccattca
     7981 tgttggaaga ccaaagggac aacgtgtgag caatatgatg attcaaggtc acgcagttga
     8041 tttaaagcag acatatacaa tttgtgtaaa taattatcgt gcagttggcg gtggtcagta
     8101 tgatatgttt atcgacgcgc cagttgtgaa agatattcaa gttgaaggcg cacaattact
     8161 tattgatttt ttatcaaata ataatttgat gcgtatcccg caagttgttg attttaaagt
     8221 tgaaaagtga cggatatatg aaaaacttac ttgattttaa tcgggtaagt ttttgttttt
     8281 gtttaaacta accactctta aacgtcaata tactttaaga aaaatgttga ttttacaaaa
     8341 aatgaggctt atattttata taacttaata ttatgaagtg attaatttgg aaacaattaa
     8401 acaagaagta agagaatcta ttgttaaagg aattatcgcg ggatataagg actatgttaa
     8461 tgagagaaat gaagtcaaga aacgtatggt gattagtgat gcctatgcct ttactaaatc
     8521 taatcatatt gaaagtcaag ttgctaagca tttggaaaat tttgttaagt atattaaaga
     8581 aaatgctgga ccatcatgga agtatttaaa gtttatattt aataaagatg aagagaaaaa
     8641 taatatcatg tttatattga aaaatgaaga ttactttgat gaaaaaaatg taagcgtagg
     8701 gaagagttta gttgctgata aaaatccaaa aagtaaaaac tacctagagg tacttatggc
     8761 taagaatagg gacattaatt ttggtactgt tggagaagat tttgaaattg ggcatcaaat
     8821 gactgcagat agtatattat ttaatataaa agaaggcagt aatagagata ttaattctta
     8881 ttttttaatt attacatatc gtattgataa agaatcaaag caattagctg caatcaaaca
     8941 atggttacca aatccagaaa caaattctgc aattatggtt gatgatttga cagagttaat
     9001 agaaaaagta atagtagaaa gagaagatta tcatattgac gaagaagagt tagaagtact
     9061 taaaaacgat ggagagttag aactcataga tgtggaacat gcatttggta tcagtatcga
     9121 tgaaggtaac gatatgattg aaagtgaaag gtgaagttaa tgtttattgg caagaattta
     9181 gaatacgtga gaaagcttaa tgctctaagt agaaaagaat tatcagaaaa aataaatgtg
     9241 agtgaacaag caatttggca atatgaaaca aaaaatatga tgccagaaat tagtaaaatt
     9301 tatgatatga catctatttt taatgttaaa tctagctact ttattagcga gcagcccgaa
     9361 gagctcctaa taaattctgt cgataaacat agtatagctt ttagagcaaa aaattataaa
     9421 gtatctacga aactattaaa taagcaatat tatcaagcaa tgtatttatc taatttaact
     9481 agctacttat tttcttttgt aacgataccg gataatatta ttttatcttt aataaataat
     9541 ttagatgaat tgttgaatgg caatttagaa tcactaaata aaaaagaaag cataaaagaa
     9601 attgctaaag ttgtgagggc taaaattctt caagatgaaa gcaacgaagc actattattt
     9661 atgcttgaaa aagcgggtat agttatatac gaaaagcgaa ttaatgatag catagacgca
     9721 tatagttttt ggagtaaaga tcagacacct tttattatac tgggtacaaa taggggggta
     9781 gcagttagaa ggaattttga tcttgcacat gagctgggac atctcgttct acatcgtcat
     9841 attcaattcg acttattatc tcctgaagaa tataagacaa ttgaacatga ggcagatatt
     9901 ttcgcctctg aatttttact acctgaagaa gcgtttaaaa aagactttga tcaaatgacc
     9961 aaaaaatcaa atcctgatta tttagctgta ttgaaagaaa aatggtatgt ttctatacaa
    10021 gcaattgcaa tgagagctta ttatctaggc ctaatgtcta gtactcagta cagatatttt
    10081 tgggcatcat taaataaaaa aggctataaa agtaaagagc ctttagacga cgtgattgaa
    10141 atgtcacgtc ctgtcaaaat gaatagttta ttaaagttgt attttgatag aaatatactg
    10201 acaccacaaa aattattgaa ttatttaaaa gtcgatgaaa catttttgaa tcatctagca
    10261 ggtattaatt taaaactctt taaggattat gttaatgaaa atagagaata taatataacg
    10321 aatctatata aataaggcac caatatttaa aactcgagct gaaaagatca t
//
LOCUS       DANBWH010000090        10312 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.093, whole genome shotgun sequence.
ACCESSION   DANBWH010000090 DANBWH010000000
VERSION     DANBWH010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10312
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.093"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            571..1324
                     /locus_tag="PPY92_001540"
                     /pseudo
     CDS             571..1324
                     /locus_tag="PPY92_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503564.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
     gene            complement(1526..1795)
                     /locus_tag="PPY92_001541"
     CDS             complement(1526..1795)
                     /locus_tag="PPY92_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625546.1"
                     /translation="MAENNQNSLVTKIATYGSFIAIASFVILFISIFLKFSFNIEFIA
                     TIMNICRYTLILGFILMSLPDVVDKNIKKIIFDAFIIIVFIFFLL"
     gene            2109..2795
                     /gene="mtnN"
                     /locus_tag="PPY92_001542"
     CDS             2109..2795
                     /gene="mtnN"
                     /locus_tag="PPY92_001542"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDJ2625547.1"
                     /translation="MIGIIGAMEEEVTILKNKLTQLSEISVAHVKFYTGILKDREVVI
                     TQSGIGKVNAAISTTLLINKFKPDVIINTGSAGALDESLNVGDVLISDDVKYHDADAT
                     AFGYEYGQIPQMPVAFQSSKPLIEKVSQVVQQQQLTAKVGLIVSGDSFIGSVEQRQKI
                     KKAFPNAMAVEMEATAIAQTCYQFNVPFVVVRAVSDLANGEAEMSFEAFLEKAAVSSS
                     QTVEALVSQL"
     gene            2815..3342
                     /locus_tag="PPY92_001543"
     CDS             2815..3342
                     /locus_tag="PPY92_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HDJ2625548.1"
                     /translation="MGLVRKFFMPNSYVQSIFQIDLDKLVDKGVKGIITDLDNTLVGW
                     DVKEPTERVKAWFKEANEKGITITIVSNNNESRVASFSQHLDIDFIFKARKPMGKAFD
                     KAITKMNIRPDQTVVIGDQMLTDVFGGNRRGLYTIMVVPVKRTDGFITKVNRLIERRL
                     LRHFSKKGYITWEEN"
     gene            3343..4443
                     /gene="yqeH"
                     /locus_tag="PPY92_001544"
     CDS             3343..4443
                     /gene="yqeH"
                     /locus_tag="PPY92_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HDJ2625549.1"
                     /translation="MSDILKCIGCGAPLQSEDKNKPGFVPEHNMFRDDVICRRCFRLK
                     NYNEVQDVGLESEDFLKLLSGLADKKGIVVNVVDVFDFEGSFINAVKRIVGNKKIILA
                     ANKLDLLPKQINKRRVKEWLKRTARKYGLEADDVVLISAEKGWGIDDLLSSIANIREN
                     EDVYIVGTTNVGKSTLINKLIEASVGEKDVVTTSRFPGTTLDMIDIPLDESSFMYDTP
                     GIIQDHQMTHLVSEKELKIIMPKKEIKQRVYQLNEAQTLFFGGLARIDYVSGGKRPLV
                     CFFSNDLNIHRTKTEKANDLWRNQLGDLLTPPGNPQNFDLNELKAVRLETGKEKRDVM
                     ISGLGFITIGPGAKVIVRVPKNVDVVLRNSIL"
     gene            4457..5263
                     /gene="aroE"
                     /locus_tag="PPY92_001545"
     CDS             4457..5263
                     /gene="aroE"
                     /locus_tag="PPY92_001545"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDJ2625550.1"
                     /translation="MKFAVIGNPISHSLSPVMHRANFNSLGLDDTYEALNIPIEDFQL
                     IKEIISKKELDGFNITIPHKERIIPYLDYVDEQAINAGAVNTVLIKDGKWIGYNTDGI
                     GYVKGLHSVYPDLENAYILILGAGGASKGIAYELAKFVKPKLTVANRTMARFESWNLN
                     INQISLADAEKYLAEFDIVINTTPAGMAGNNESIINLKHLSPNTLMSDIVYIPYKTPI
                     LEEAERKGNHIYNGLDMFVYQGAESFKIWTNKDADINSMKTAVLQQLKGE"
     gene            5267..5557
                     /gene="yhbY"
                     /locus_tag="PPY92_001546"
     CDS             5267..5557
                     /gene="yhbY"
                     /locus_tag="PPY92_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDJ2625551.1"
                     /translation="MLTGKQKRYLRSLAHNIDPIFQIGKGGINENMIKQIDDTLENRE
                     LIKVHVLQNNFDDKKELAETLSEATRSELVQVIGSMIVIYRESKENKEIELP"
     gene            5560..6129
                     /gene="nadD"
                     /locus_tag="PPY92_001547"
     CDS             5560..6129
                     /gene="nadD"
                     /locus_tag="PPY92_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HDJ2625552.1"
                     /translation="MKKIVLYGGQFNPIHTAHMIVASEVFHELQPDEFYFLPSFMSPL
                     KKHNNFIDVQHRLTMIQMIIDELGFGDICDDEIKRGGQSYTYDTIKAFKEQHKDSELY
                     FVIGTDQYNQLEKWYQIEYLKEMVTFVVVNRDKNSQNVENAMIAIQIPRVDISSTMIR
                     QRVSEGKSIQVLVPKSVENYIKGEGLYEH"
     gene            6119..6703
                     /gene="yqeK"
                     /locus_tag="PPY92_001548"
     CDS             6119..6703
                     /gene="yqeK"
                     /locus_tag="PPY92_001548"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDJ2625553.1"
                     /translation="MNIEKAKRLAKEKLPEKRYNHSLRVAETAIKLAEIYDGDTSKVE
                     LAGVLHDFCKYDDLGKMYQIVRQYELGNDLLSYGSEILHGPVCAAIMEHEYGINDEEV
                     LMAIKYHTTGRQQMTKTEKLIFIADYIEPGRTIPGVDDIRDMAYNQGSLDKTIYEISK
                     RTVLFLIQKDITVYNKTIDCLNYYNYSDERIKDD"
     gene            6704..7057
                     /gene="rsfS"
                     /locus_tag="PPY92_001549"
     CDS             6704..7057
                     /gene="rsfS"
                     /locus_tag="PPY92_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDJ2625554.1"
                     /translation="MNSQELLAIAVDAIDNKKGEDTISLEMKGISDMTDYFVVTHGNN
                     ERQVQAIARAVKEVANEQNIEVKRMEGYNEARWILIDLADVVVHVFHKDERNYYNIEK
                     LYQDAPLESYGQVAY"
     gene            7060..7776
                     /locus_tag="PPY92_001550"
     CDS             7060..7776
                     /locus_tag="PPY92_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDJ2625555.1"
                     /translation="MSQYAEMSLVYDQLTQDQPYEKWFEIVKNHCKDESNILDIGCGT
                     GSLTVQLEALGNVTGMDLSVDMLTVAAQKSANVNWLEGDMTSFDLQQQFDIITIFCDS
                     LNYLQDETAVIETFINVYHHLTDSGVFIFDVHTVYKMMTLFNNQSYIDDKGDIFLAWD
                     AVQGDLPLSVYHDMTFFIRHEDETYSRFDESHFQRTFDEKTYLSWLAQVGFKHVETFT
                     DFNIDEHNEDTERLFFIAKK"
     gene            7816..8502
                     /locus_tag="PPY92_001551"
     CDS             7816..8502
                     /locus_tag="PPY92_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001802022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HDJ2625556.1"
                     /translation="MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYT
                     SRNFENKDTALKQSTSENNSLSKLEDVQVKDGDNSKNKGPVYVDVKGAVKHPNVYKMT
                     SKDRVVDLLDKAQLLDDADVSRINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHVKN
                     GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKT
                     FDKLKSYFTI"
     gene            8594..9055
                     /locus_tag="PPY92_001552"
     CDS             8594..9055
                     /locus_tag="PPY92_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HDJ2625557.1"
                     /translation="MERIKWEEYFMAQSHLLALRSTCQRLSVGATIVKDNRIIAGGYN
                     GSVAGEVHCIDEGCLIEDGHCIRTIHAEMNALLQCAKQGVSTEGATIYVTHFPCLNCT
                     KSIIQAGIKRIYYAEDYHNHEYATKLLKQSGIEFKKIPFSPEYVAKYLTKG"
     gene            9060..>10312
                     /locus_tag="PPY92_001553"
     CDS             9060..>10312
                     /locus_tag="PPY92_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HDJ2625558.1"
                     /translation="MLYVALSMIVGVLWNSSKVLSTFLFILLLYITYRKNKIVYAPIS
                     LFLIIFSSWYLHYSQQAIFNYINYIERNSQFNERAQVIQIQRQGSDTYKGRLSLKNEI
                     YPFFLTDKKNFDLKKIESRNCIVKGQFKVNENKFVTLKLQSIVVQSCLESNRSNLIEK
                     HKQFIMNRIYDSGIKFPDRIMALITGDVKEINEQFKERVKEIGIYHLLAVSGSHIAAI
                     VFLIYQPLKRLNLPLFVIKGITIIVLTLYAQYTNYAPSAVRAIIMTTLVLLITKQIKI
                     KGIQLLAFAFIIMFILNPLVVYDIGFQFSFIISFFIMLLFPFLQQLSKLQSLFIITFI
                     AQLASFIVAIPNFHQLQWVGFLSNLIFVPYYSIILFPLSILFFITSHFIVGLTPLNYL
                     VDLSFNFHDWLLDLFTRIKQSHFSVP"
BASE COUNT         3740 a         1319 c         1878 g         3375 t
ORIGIN      
        1 attttttctg atatactgtt aaacggtttg gaatttttat gtttacactg tattggttga
       61 tataactcaa ctaataattg cgaacagggt atttcaaatt ggaaagtatt atgaacgtga
      121 tacataatca aaattgtagg ctcaagggag tgggacagaa atgataaaga gccactaatg
      181 atttattatg tagtggttct tacacattag ccacagctaa tgtgtactta aaaataggaa
      241 tacatgagta aaactcatgt ataagaaata ctaatttcta tagaaaaagt atttctttat
      301 cgtcgtccca cccaacttgc acattattgt aagctgactt tccgccagct tctatgttgg
      361 ggccccgccc caacttgcat tgcctgtaga atttcttttc gaaattctct gtgttggggc
      421 ccctgactag aattgaaaaa agcttgttac aagtgcattt tcgttcagtc aactactgcc
      481 aatataactt cgtagagcat agaacattga tttatgtccc agcctgttta tcatttatgt
      541 ctcgctctca aatgtaaatt aataattgtt ttgggggagt ttgaagttaa atatttaaca
      601 ggatttattt taatattatt gttagaagga atttttacaa attcagcgag tgcaatcgaa
      661 tattcagact tacatcataa aagtaagttt gattcaaagc gtctaagtaa tgctaagatg
      721 tcattcatca atccaactca gcttgaaaat aaaaacacaa acgatagact gttgaagcat
      781 gatttgttat ttcatgacat gttcgtaaat gttgcttcga aaaaggattt taaagttgaa
      841 ttcgaaaatg aggcactttc aaagaaattt ataaataaga atatcgatat atatgctgga
      901 agctatagct atgaatgtca tgggggagca accaataaaa cgcaatgtag ttatggtggt
      961 gttactttaa gtgacaataa taaataagat gattataaga atataccttc taatttatgg
     1021 attgacggac atcaaacaga aatagaacta actgcagtaa aaacgaaaaa agaaaattgt
     1081 taccattcaa gaattagatg ttcaattaag aaattatttg aatgagaagt ataagttgta
     1141 cgaacaaggt ggcgacattg ttaaagggta tgttaaatat cataatgatg atgaacaaaa
     1201 tgtagaatat gatttttata atttaaatgg tgagtatggt tatgaggtat taaaaatgta
     1261 tgctgataat aaaactatca atagagacaa attgcattta gatatctatt tattcaaatc
     1321 ataatttaat taagttaatc attaataatc tttgacagtg aaatataaag tacctgacat
     1381 tatattgtta caaatgaaaa cagcagtaag gatgctaaca cttagaaaag tcttactgct
     1441 attttctatt agaattatct ttaatgcaca caggaattcg atacgaaaca aatatgatta
     1501 actgtgtaat tacaaaataa aactactata gtaaaaagaa gataaagacg atgataataa
     1561 atgcatcgaa tataattttc ttgatgtttt tatcaacaac atcaggtaag gacataagta
     1621 taaatcctag aattagagtg tagcgacata tgttcataat tgttgctata aactcgatat
     1681 taaatgaaaa ctttaaaaat atacttataa ataaaatgac aaacgacgca attgcaataa
     1741 aactgccgta tgtagctatc tttgttacga gactattttg attattttca gccattgtaa
     1801 aatcctctta tttcatcatt attattttaa acatatcatt ttcctgttat ttaataaagt
     1861 ttcttgacac gattggttga tgtaattgat atgattagtt tttgaaaaat agacaaatta
     1921 gaagcatcga attcactgtt atgagttcaa aaataattat ttatgatacc aagttattga
     1981 aagagctttt tataaaaata aagcaaaagt tttatacagt aatgaaaatc atgatacttg
     2041 tcgttgtagt gattaaaatg tgataaaata acaaatgcta ggatttttta aaagaggtga
     2101 atcaaaggat gattggtata attggtgcca tggaagaaga agtaacaata ttaaaaaata
     2161 aattaacaca attaagcgaa atttcagttg cacatgttaa attttatact ggcattttaa
     2221 aagatagaga agtagtgatt acccaaagtg gcattggaaa agttaatgct gcaatttcta
     2281 cgacattatt aattaataag tttaaaccgg acgtcattat taatacaggt tctgctggag
     2341 ctttagatga aagtttaaat gtaggtgacg ttcttataag tgatgatgta aaatatcatg
     2401 atgcggatgc aacagcattt ggttatgaat atggacaaat accacagatg ccggtagcat
     2461 ttcaatcaag taaaccttta atagaaaaag tatctcaagt tgtacaacaa caacaattaa
     2521 cagctaaagt aggcttaatt gtaagtggtg atagctttat cggtagtgtt gaacaacgcc
     2581 aaaaaattaa aaaagcattt ccaaatgcga tggcggttga aatggaagca actgcaattg
     2641 cacaaacatg ttatcaattt aatgtaccat ttgttgtagt tcgtgcagtt tcagacttag
     2701 caaatggaga agcggaaatg agcttcgaag catttttaga aaaagcagct gtatcatcaa
     2761 gtcaaactgt tgaagcatta gtgtctcaat tataaaaagg taaaggtgag ttaaatgggt
     2821 ttagttcgca agttttttat gccgaattca tatgttcaat caatatttca aattgattta
     2881 gacaagttag tggacaaagg cgttaaaggt attattacag atttagataa tacgctagta
     2941 ggttgggatg ttaaagaacc tacagaacgt gttaaagcat ggtttaagga agctaatgaa
     3001 aaaggaatca ctattacaat cgtgtctaat aataatgagt ctcgtgttgc tagttttagt
     3061 cagcatttag acatcgattt tatttttaaa gcgagaaagc caatggggaa agcgtttgat
     3121 aaagcaataa ctaagatgaa tatcagacca gatcaaactg ttgttatagg tgaccaaatg
     3181 cttactgatg tatttggtgg taatcgtcga ggtctatata caattatggt tgttccagtt
     3241 aaacgaactg atggctttat tactaaggtt aatagattaa ttgaaagacg attattacgt
     3301 catttcagta aaaaaggtta tatcacatgg gaggaaaatt gattgtctga cattttaaaa
     3361 tgtatcggtt gtggtgcgcc acttcaatct gaagataaaa ataaacctgg ttttgtacca
     3421 gagcataata tgtttcgtga tgacgtgatt tgcagacgtt gtttccgctt gaaaaattat
     3481 aacgaagttc aagatgtagg attagaaagt gaagactttt taaaattatt atcaggactt
     3541 gcggataaaa agggtattgt cgtcaatgtc gtggatgtat ttgactttga aggatcattt
     3601 attaatgcag ttaaacgtat tgtcggaaac aaaaaaatca ttttagcagc aaataaattg
     3661 gatttattgc cgaaacaaat taataaacgt cgagttaaag aatggttaaa acgaacagca
     3721 agaaaatatg gtttggaagc tgacgatgtc gtattaattt cagctgaaaa aggctggggc
     3781 atagacgact tattatcatc aattgcgaat attcgagaaa atgaagatgt gtatattgta
     3841 gggacaacga atgttgggaa atctacattg attaataaac tgattgaagc tagtgttggt
     3901 gaaaaagatg tagtaacaac ttcaagattc cctggaacaa ctttagatat gatagatatt
     3961 cctttagatg aatcatcatt tatgtatgat acaccaggta ttattcaaga tcatcaaatg
     4021 acgcatttag ttagtgaaaa agaattgaaa attattatgc ctaagaaaga aataaaacaa
     4081 cgcgtatatc aattaaatga ggcgcagaca ttattcttcg gcggtctagc gcgcatagat
     4141 tatgtatcag gtggtaaacg tccgttagtt tgtttctttt ctaatgactt gaatatacat
     4201 cgtactaaaa cggagaaggc taatgattta tggcgtaatc aacttggcga tttattaacg
     4261 ccacctggaa atccacaaaa ttttgatctt aatgagttaa aggctgttag acttgaaaca
     4321 ggcaaagaga aacgtgatgt tatgatctct ggtctaggct ttataactat aggaccagga
     4381 gctaaagtaa tcgttcgtgt tcctaaaaat gttgatgttg tattaagaaa ttctatttta
     4441 taaggtgatt taaaaaatga aatttgcagt tatcggaaat cctatttcac attccttgtc
     4501 gcccgttatg catagagcaa attttaattc tttaggatta gatgatactt atgaagcttt
     4561 aaatattcca attgaagatt ttcagttaat taaagaaatt atttcgaaaa aagaattaga
     4621 tggctttaat atcacaattc ctcataaaga acgtatcata ccgtatttag attatgttga
     4681 tgaacaagcg attaatgcag gtgcagttaa cactgttttg ataaaagatg gcaagtggat
     4741 agggtataat acagatggta ttggttatgt taaaggattg cacagcgttt atccagattt
     4801 agaaaatgca tacattttaa ttttgggcgc aggtggtgca agtaaaggta ttgcttatga
     4861 attagcaaaa tttgtaaagc ccaaattaac tgttgcgaat agaacgatgg ctcgttttga
     4921 atcttggaat ttaaatataa accaaatttc attggcagat gctgaaaagt atttagctga
     4981 attcgatatc gttattaata caacaccagc gggtatggct ggaaataacg aaagtattat
     5041 taatttaaaa catctttctc ccaatacttt aatgagtgat attgtttata taccgtataa
     5101 aacacctatt ttagaggaag cagagcgcaa gggaaaccat atttataatg gcttagatat
     5161 gtttgtttac caaggtgcgg aaagctttaa aatttggact aataaagatg ctgatattaa
     5221 ttctatgaaa acagcagttt tacaacaatt aaaaggagaa taacatatgc ttactggcaa
     5281 acaaaaaaga tacttaagaa gtttagcaca caatattgat ccgatttttc aaattggaaa
     5341 aggcggtatc aacgaaaata tgattaaaca aatagatgat acgttagaaa acagagaatt
     5401 gattaaagta catgtactac aaaataactt tgatgataaa aaagaattag ctgaaacatt
     5461 aagcgaagct acgcgtagtg aattagtgca agtgattgga tctatgatag tgatttatag
     5521 agaatctaaa gaaaataaag aaattgaatt gccataataa tgaaaaagat agtactttac
     5581 ggcggtcagt ttaaccctat ccatactgca catatgatag tagctagcga agtatttcat
     5641 gaattacagc cagatgaatt ttatttttta cctagtttta tgtctccatt gaaaaagcac
     5701 aataatttta tagatgttca gcacagatta acaatgatac agatgattat cgacgagctt
     5761 ggttttggag atatttgcga cgatgaaatt aaacgtggtg gtcagagtta tacctatgac
     5821 acgatcaagg cattcaagga gcaacacaaa gacagtgagt tgtactttgt tattgggacg
     5881 gatcagtata accaactaga gaaatggtat caaattgaat acttaaaaga aatggttact
     5941 tttgtagttg taaatcgaga caaaaatagt caaaatgttg aaaatgctat gattgcaatt
     6001 cagataccta gggtagatat aagttcgaca atgattcgac aaagagttag tgaagggaaa
     6061 tctatccaag ttcttgttcc taaatccgtt gaaaactata ttaaggggga aggattatat
     6121 gaacattgaa aaagcaaaac ggcttgcaaa agagaaactg ccagagaaac gttataatca
     6181 ttctttaaga gttgcagaaa cagcgattaa attggcagaa atttatgatg gagacactag
     6241 taaagtagaa ttagcaggtg tattacatga tttctgtaaa tatgatgatt taggtaaaat
     6301 gtatcaaatt gttcgacaat acgaattagg taatgatcta ctgagttatg gtagtgaaat
     6361 attgcatggc cctgtgtgtg cagcaatcat ggaacatgaa tatggtatca atgatgagga
     6421 agtattaatg gctatcaaat accatactac tggacgtcaa caaatgacaa aaactgaaaa
     6481 actgattttt attgctgatt acatcgaacc tggaagaaca atcccaggag ttgatgatat
     6541 tcgagatatg gcatacaatc aaggtagttt agataagaca atttatgaaa tttctaaacg
     6601 cacagtacta tttttaatac agaaagatat tacggtatat aataagacga ttgactgttt
     6661 aaattattat aactatagtg acgaaagaat aaaggatgat taaatgaatt cacaagaatt
     6721 attagcaatt gctgtggatg caattgacaa taaaaaaggc gaagatacga tttctttaga
     6781 aatgaaaggt atcagcgata tgacagatta ttttgttgta acgcacggaa ataatgaacg
     6841 acaagttcaa gcgattgcta gagcggtgaa agaagtagcc aatgaacaaa atatagaagt
     6901 aaaacgtatg gaaggataca atgaagcgcg ttggatatta attgacttag ctgatgttgt
     6961 ggtacatgtt ttccataaag acgaaagaaa ttattataat attgaaaagt tatatcaaga
     7021 tgcaccatta gaatcatatg gtcaggttgc gtattaatta tgtcgcaata tgcagaaatg
     7081 agcctagtgt acgatcaatt gactcaagat caaccatatg aaaaatggtt tgaaattgta
     7141 aaaaatcact gcaaagatga atcaaatatt ttagatattg gatgcggtac tggtagttta
     7201 acagttcaat tagaagcttt aggtaatgtt actggtatgg atttaagtgt tgatatgttg
     7261 actgttgcag ctcaaaaatc agcaaatgta aattggcttg aaggagatat gacatctttt
     7321 gatttgcaac aacaatttga tatcatcact attttttgtg actctctaaa ttatttgcaa
     7381 gatgaaactg cagtgattga aacattcatc aatgtttatc atcatctgac tgattcaggt
     7441 gtatttattt ttgatgtaca tactgtttat aaaatgatga ctttgtttaa taatcaaagt
     7501 tatattgatg ataaagggga cattttttta gcatgggatg ctgtgcaagg agatttacct
     7561 ttaagtgttt atcatgatat gacatttttc attcgacatg aagatgaaac atattcaaga
     7621 tttgatgagt ctcattttca gagaacattt gatgaaaaga catatttgtc ttggttagct
     7681 caagtcggtt ttaaacatgt tgaaacattt actgatttta atatagatga acataatgaa
     7741 gatacagaaa gattgttttt cattgcgaaa aaataaaagt aaaaccactc gctcagacgt
     7801 gtataaagtt gaggagtggt tttattgtat caatttttat tacgctataa agatttttta
     7861 actcagtgga agttatatat tataagtgct gttgttttaa ttatggtatt aattggtttt
     7921 atattctgga gacaagatga ttatacttca agaaattttg aaaataaaga tactgctctg
     7981 aaacaaagca ctagtgaaaa taatagtttg tccaaattag aagatgtcca ggtcaaagat
     8041 ggagataatt ccaaaaataa aggtcctgta tatgtcgatg taaaaggtgc tgttaaacat
     8101 cctaatgttt ataaaatgac atctaaggat agagtagttg atttacttga taaagcacaa
     8161 ttattggatg atgcagatgt aagtcgaatt aatttgtctg aaaaattaac agatcaaaaa
     8221 atgattttca tacctcataa aggacaaaag aatgttgaac cacaaattga agtaaacagt
     8281 gtgcacgtaa aaaatgggaa cacaaataat actaaagtaa atttaaatac cgcatctgta
     8341 tcagaattga tgtctgttcc tggagtaggg caagctaaag ctaatgcaat tgttgaatat
     8401 cgcaaccaac aaggtgcatt tcaagaaatt gacgatttga aaaaagtaaa aggttttgga
     8461 agtaaaactt ttgataaact gaaatcttat ttcacgatat aatggattat tttaattgca
     8521 aattttattt tttgtgatta atcttatatt attagaaaat gttactaaat tttaagtatg
     8581 gaaggtgaac atcttggaaa gaatcaaatg ggaagaatat tttatggcac aaagtcattt
     8641 gctagcatta cgttcaactt gtcaaagatt atctgtaggt gcaacgattg ttaaggataa
     8701 tcgtattatt gctggtggtt ataatggctc tgtagctggc gaggtgcatt gtatagatga
     8761 aggatgttta attgaagatg gacattgtat cagaacgata catgcagaaa tgaatgcttt
     8821 attacaatgt gcaaaacaag gtgtatctac tgaaggtgca acaatctatg ttactcattt
     8881 tccatgccta aattgtacaa agtcaattat tcaagcaggt ataaagcgta tctactatgc
     8941 agaagattat cataaccatg aatatgcaac taaattactc aaacaatctg gtattgaatt
     9001 taaaaaaatt ccattttcac cagaatatgt tgctaaatat ctgactaaag gttaatgttt
     9061 tgctgtatgt cgcgttatca atgattgtag gagtgctttg gaattctagc aaagtgctct
     9121 ctacatttct tttcatttta cttttgtata ttacttatcg taaaaataaa atcgtttatg
     9181 cccctatttc tctcttttta atcattttct cctcatggta tttacattat tcacaacaag
     9241 caatatttaa ttatatcaat tatattgaac gtaattctca gtttaatgag cgtgctcaag
     9301 taatccaaat tcaacgtcaa ggtagtgaca catataaagg taggttgagt ttaaaaaatg
     9361 aaatatatcc tttcttttta acagataaaa agaattttga tttaaagaaa attgaaagtc
     9421 gtaattgtat tgttaaagga caattcaaag ttaatgaaaa taagtttgta actcttaaat
     9481 tacaaagtat agttgtacaa agctgcctag aatcgaaccg gtctaattta attgagaaac
     9541 ataaacagtt tataatgaat cgaatttatg attcgggtat taagtttccg gatcgtatta
     9601 tggcattgat tactggtgac gtaaaagaaa ttaatgagca atttaaggaa cgtgttaaag
     9661 agataggtat atatcatttg ctggcagtta gtggctcgca tatagctgca attgtattct
     9721 taatttacca acctttaaaa cgattaaatt tacctttatt tgtcattaaa ggaattacaa
     9781 tcattgtatt aactttatat gctcaataca caaattatgc acctagtgct gtaagagcta
     9841 taataatgac aactcttgta ctgcttatta ctaagcaaat taaaataaag ggtattcagc
     9901 tattagcatt tgcatttata attatgttta ttttaaaccc actagttgtt tatgatattg
     9961 gatttcaatt ttcattcatc atttcatttt ttattatgct actttttcct tttttacagc
    10021 aattgtcaaa gttacaatca ttattcataa ttacgtttat tgcacaatta gcttcattta
    10081 tcgttgccat tccaaacttt catcaacttc aatgggtggg atttttatct aatttgattt
    10141 ttgtaccgta ctattcgatt atattgtttc cgctatctat tttattcttt attacaagtc
    10201 attttattgt gggattaacg ccgctaaatt acttggttga cctaagtttt aattttcatg
    10261 actggttact agacctattc acaagaatta agcaatcaca tttttctgtt cc
//
LOCUS       DANBWH010000091        10243 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.094, whole genome shotgun sequence.
ACCESSION   DANBWH010000091 DANBWH010000000
VERSION     DANBWH010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10243
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.094"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            105..1073
                     /gene="pfkA"
                     /locus_tag="PPY92_001554"
     CDS             105..1073
                     /gene="pfkA"
                     /locus_tag="PPY92_001554"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002462395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDJ2625559.1"
                     /translation="MKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGL
                     LNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIEGLVVIGG
                     DGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTAS
                     SHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHS
                     IVLVAEGCMTAQDCQKELSQYINVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDL
                     LMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKLSI"
     gene            1095..2852
                     /gene="pyk"
                     /locus_tag="PPY92_001555"
     CDS             1095..2852
                     /gene="pyk"
                     /locus_tag="PPY92_001555"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDJ2625560.1"
                     /translation="MRKTKIVCTIGPASESEEMIEKLINAGMNVARLNFSHGSHEEHK
                     GRIDTIRKVAKRLDKIVAILLDTKGPEIRTHNMKDGIIELERGNEVIVSMNEVEGTPE
                     KFSVTYENLINDVQVGSYILLDDGLIELQVKDIDHAKKEVKCDILNSGELKNKKGVNL
                     PGVRVSLPGITEKDAEDIRFGIKENVDFIAASFVRRPSDVLEIREILEEQKANISVFP
                     KIENQEGIDNIAEILEVSDGLMVARGDMGVEIPPEKVPMVQKDLIRQCNKLGKPVITA
                     TQMLDSMQRNPRATRAEASDVANAIYDGTDAVMLSGETAAGLYPEEAVKTMRNIAVSA
                     EAAQDYKKLLSDRTKLVETSLVNAIGISVAHTALNLNVKAIVAATESGSTARTISKYR
                     PHSDIIAVTPSEETARQCSIVWGVQPVVKKGRKSTDALLNNAVATAVETGRVSNGDLI
                     IITAGVPTGETGTTNMMKIHLVGDEIANGQGIGRGSVVGTTLVAETVKDLEGKDLSDK
                     VIVTNSIDETFVPYVEKALGLITEENGITSPSAIVGLEKGIPTVVGVEKAVKNISNNM
                     LVTIDAAQGEIFEGYANVL"
     gene            complement(3200..4561)
                     /locus_tag="PPY92_001556"
     CDS             complement(3200..4561)
                     /locus_tag="PPY92_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDJ2625561.1"
                     /translation="MAEKLQRELSNRHIQLIAIGGAIGTGLFLGAGQTIALTGPSILL
                     TYIIIGFMLFMFMRGLGEIIIQNTEFKSFADVTNTYIGPFAGFVTGWTYWFCWIITGM
                     AEVTAVAKYVSFWFPEIPNWISALFCVLLLMSFNLLSARLFGELEFWFSIIKIATIIG
                     LIVVGFVMILFAFKTQFGHASFTNLYEHGIFAKGASGFFMSFQMALFSFVGIEMIGVT
                     AGETKDPVKTIPKAINSVPIRILIFYVGALAVIMSIIPWQQVDPDNSPFVKLFALIGI
                     PFAAGLINFVVLTAAASSCNSGIFSNSRMLFGLSSQQQAPPNFSKTNKYGVPHVAIFA
                     SSALLLVAALLNYIFPDATKVFTYVTTISTVLFLVVWGLIIIAYINYSRKNPDLHKNA
                     TYKLLGGKYMGYLIFVFFIFVFGLLFINVDTRRAIYFIPIWFILLAFMYLRYKRIAAK
                     SNK"
     gene            4909..6030
                     /locus_tag="PPY92_001557"
     CDS             4909..6030
                     /locus_tag="PPY92_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001662816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HDJ2625562.1"
                     /translation="MAELQRGLEGVIAAETKISSIIESQLTYAGYDIDDLAENAQFEE
                     VIFLLWNYRLPNEEELAHLKGKLNQYMTLNPRVYTHFEEYVTDHVHPMTALRTSLSYI
                     AHFDPDAENESDENRYERAMRIQAKVASLVTAFARVRQDKEPLKPNPDLSYAANFLYM
                     LRGELPTDIEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIVAAVGSLKGPL
                     HGGANEQVMTMLSEIGSIENVDAYLDEKFANKDKVMGFGHRVYKDGDPRAKYLREMSR
                     QITKDAGREELFEMSVKMEKRMAEEKGLIPNVDFYSASVYHCMEIPHDLFTPIFAVSR
                     SAGWIAHILEQYKDNRIMRPRAKYIGETNRKYIPLEERK"
     gene            6079..7347
                     /gene="icd"
                     /locus_tag="PPY92_001558"
     CDS             6079..7347
                     /gene="icd"
                     /locus_tag="PPY92_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDJ2625563.1"
                     /translation="MTAEKITQGTEGLNVPNEPIIPFIIGDGIGPDIWKAASRVIDAA
                     VEKAYNGEKRIEWKEVLAGQKAFDTTGEWLPQETLDTIKEYLIAVKGPLTTPIGGGIR
                     SLNVALRQELDLFTCLRPVRWFKGVPSPVKRPQDVDMVIFRENTEDIYAGIEFKEGTT
                     EVKKVIDFLQNEMGATNIRFPETSGIGIKPVSKEGTERLVRAAIQYAIDNNRKSVTLV
                     HKGNIMKFTEGSFKQWGYDLALSEFGDQVFTWQQYDEIVENEGRDAANAAQEKAEKEG
                     KIIIKDSIADIFLQQILTRPAEHDVVATMNLNGDYISDALAAQVGGIGIAPGANINYE
                     TGHAIFEATHGTAPKYAGLNKVNPSSVILSSVLMLEHLGWQEAADKITDSIEDTIASK
                     VVTYDFARLMDGAEEVSTSAFADELIKNLK"
     gene            7737..8441
                     /locus_tag="PPY92_001559"
     CDS             7737..8441
                     /locus_tag="PPY92_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDJ2625564.1"
                     /translation="MSQKVLVVDDEHSIVTLLKYNLETAGYEVVVAFDGDEALEKVES
                     EQPDLIILDVMLPKKDGIDVCKTVRTNKNLVPILMLTAKNDEFDRVLGLELGADDYMT
                     KPFSPREVVARVKAILRRSQFVKEVEEIDDDITIGSIRIRPDYFEVYKHNELLELTPK
                     EFELLLYLIERQGRVITREHMLNTVWNYEFAGDSRIVDVHISHLRDKLEDNPKKPQLI
                     KTVRGLGYKLERPKEQ"
     gene            8441..10102
                     /locus_tag="PPY92_001560"
     CDS             8441..10102
                     /locus_tag="PPY92_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HDJ2625565.1"
                     /translation="MKFHHRLMLLISSILIISFVTLGIIIHASITYVLSEKHEQALAT
                     DARSYIYLVQDNEIDKVKEIVKQQNIDLLITKHDKKVFSSGKLKAIPNSDSYIQKRDV
                     FIFNKKVNGYHVWIKGYNNDITEMHWTLWKYLILTCLVVLICLYFASRSFKRTLIRPI
                     QEVTYATQLLANGYYHIRVPESNVVETKALFVSTNDLARRLQKLNNEQKIQSNRLKTT
                     IENIPSAILMIDRNGKIVVANKAYYEQFNISHNIEQVGYHGYVNTEIEQLILESFKVE
                     KPIYEQLEVAINQVHAKYFDISCVPILTRSQKSLQGILVVMHDITNLKQLENLRREFV
                     ANVSHELKTPITSIKGFAETLIDGAKNDAESLDMFLNIILKESNRIESLVTDLLDLSH
                     IEQHTELDTDYMNLSDLTRRIIDNMMTQANQKNISIHTDIEKDVIVKAQESKIAQVIT
                     NLLTNAINYSYEDGDINVRVYRDDFRVIFEVQDFGIGIKLEDQQRIFERFYRVDKARS
                     RDSGGTGLGLSITKHIVEAHQGNIEVNSQVGKGSTFKVILKDYKE"
BASE COUNT         3666 a         1436 c         1983 g         3158 t
ORIGIN      
        1 taaaacgttt acatcaattc aatacattag ttttgatgga atgacatatc aatttgtggt
       61 aatttagagt taaagataaa tcagttatag aaaggtatgt cgtcatgaag aaaattgcag
      121 ttttaactag tggtggagat tcacctggaa tgaatgctgc cgtaagagca gttgttcgta
      181 cagcaattta caatgaaatt gaagtttatg gtgtgtatca tggttaccaa ggattgttaa
      241 atgatgatat tcataaactt gaattaggat cagttgggga tacgattcag cgtggaggta
      301 cattcttgta ttcagcaaga tgtccagagt ttaaggagca agaagtacgt aaagttgcaa
      361 tcgaaaactt acgtaaaaga gggattgagg gccttgtagt tattggtggt gacggtagtt
      421 atcgcggtgc acaacgcatc agtgaggaat gcaaagaaat tcaaactatc ggtattcctg
      481 gtacgattga caatgatatc aatggtactg attttacaat tggatttgac acagctttaa
      541 atacgattat tggcttagtc gacaaaatta gagatactgc gtcaagtcac gcacgaacat
      601 ttatcattga agcaatgggc cgtgattgtg gagatctagc attatgggct ggattatcag
      661 ttggtgctga gacaattgta gttccagaag tgaaaacaga tattaaagaa atagctgata
      721 aaattgaaca aggtattaaa cgtggtaaga aacactcaat cgttcttgta gcagaaggtt
      781 gcatgactgc gcaagattgt caaaaagaat tatcacaata catcaatgtt gataatagag
      841 tgtctgtgtt aggtcacgtt caacgtggtg gtagcccaac aggtgcggat agagttttag
      901 catcacgttt aggtggatat gcggtagact tattaatgca aggtgaaaca gctaagggtg
      961 ttggaattaa gaacaataaa attgtagcaa catcttttga tgaaattttt gatggtaaag
     1021 atcataaatt tgattatagt ctatatgaac ttgctaacaa gttatctata taagatttca
     1081 ggaggaatta taaaatgaga aaaactaaaa ttgtatgtac aattggacca gcttcagaat
     1141 cagaagaaat gattgagaaa ttaatcaatg ctggtatgaa cgttgcacga ttaaactttt
     1201 cacatggtag tcatgaagag cataaaggta gaattgatac aattcgtaaa gtagctaaaa
     1261 gattagacaa aattgtagca attttattag atacgaaagg tccagaaatt cgtacgcata
     1321 atatgaaaga cggtatcatt gaacttgaac gtggtaacga agttattgtt agcatgaatg
     1381 aagttgaagg aacacctgaa aagttctcag taacatatga aaacttaatt aacgatgttc
     1441 aagtaggttc atacatttta cttgatgatg gcttaattga attacaagtt aaagatattg
     1501 accatgctaa aaaagaagtt aaatgtgata ttttaaactc tggtgagctt aaaaacaaaa
     1561 aaggtgttaa cttacctggt gtaagagtaa gtttacctgg tattacagaa aaagatgctg
     1621 aagatatccg tttcggtatt aaagaaaatg ttgacttcat tgcagcaagt ttcgtacgtc
     1681 gtcctagtga tgttttagaa attcgtgaaa ttttagaaga acaaaaagct aacatttcag
     1741 tattccctaa aattgaaaac caagaaggta ttgataatat tgcggaaatt cttgaagtgt
     1801 ctgatggttt aatggttgca cgtggtgaca tgggtgttga aattccacct gaaaaagtac
     1861 caatggttca aaaagattta atcagacaat gtaacaaatt aggtaaacca gttattacag
     1921 ctacacaaat gttagattct atgcaacgta acccacgtgc tacacgtgca gaagctagtg
     1981 acgttgccaa cgcaatctat gatggtacag atgcagtaat gttatctggt gaaactgctg
     2041 ctggtttata tcctgaagaa gctgttaaaa caatgagaaa tattgctgta tcagctgaag
     2101 cagcccaaga ttataaaaag ttattgtcag atcgtactaa attagttgaa acttcattag
     2161 tgaatgctat cggtatttcg gttgcacata cagctttaaa cttaaatgtt aaagcaattg
     2221 tagctgctac tgaaagtggt tcaacggcac gtactatctc taaatatcgt ccacattcag
     2281 acattattgc ggtgactcca agtgaagaaa cagcacgtca atgttcaatt gtttggggag
     2341 ttcaacctgt agttaaaaaa ggacgtaaga gtacagatgc attgttaaac aatgcagttg
     2401 caacagctgt tgaaactggt agagtatcta atggtgattt aatcattatt actgctggtg
     2461 taccaactgg tgaaactgga actactaata tgatgaaaat ccacctagtt ggtgacgaaa
     2521 ttgctaatgg tcaaggtatt ggacgtggat cagttgttgg tactacgtta gttgctgaaa
     2581 ctgttaaaga tttagaaggt aaagatttat ctgacaaagt tatcgttact aactcaatcg
     2641 atgaaacgtt tgtaccttat gtagaaaaag ctttaggatt aattacagaa gaaaatggta
     2701 ttacatcacc aagtgcaatt gttggtttag aaaaaggtat tccaacagtt gtaggtgtag
     2761 aaaaagctgt taaaaacata agcaataaca tgttagttac gattgatgct gctcaaggtg
     2821 aaatctttga aggatatgca aacgtactat aatttataaa aaaacgtctt tccatttatc
     2881 aacaatggaa aggcgttttt tggttcatct ggtattttat gacgtaatta ataggttatt
     2941 tgataatgat agtgtatgaa tggcaatcta tataaatgtt tatatctttt atacatgtac
     3001 attatcacct tcaaactttc actcatatta ctttggaaat ttattataaa atagaagtat
     3061 ggatgtattt ctgaaatgat acattattaa atagatgaga aagtaaaagt tttgagccaa
     3121 gtacgcaatt taatattata agttgcatat aaaaacccct gttgaacttt agtatattca
     3181 acaggggtaa aacttgttgt tatttatttg atttagcagc gatacgttta tatcttaagt
     3241 acataaatgc taaaagtata aaccaaatcg gaataaaata aattgcacgt cttgtatcaa
     3301 cattaataaa taataacccg aacacaaaaa tgaagaatac aaatattaag tagcccatat
     3361 atttaccacc taatagtttg tacgtagcat ttttatgtag atctgggttt ttacgactat
     3421 aattgatata tgcaatgata atcagacccc atacaactaa aaataacact gtagagatgg
     3481 tagtcacata cgtaaatact tttgtcgcat ctgggaaaat atagtttagt aatgctgcca
     3541 caagtaataa tgctgatgaa gcaaagattg caacatgtgg aacgccatat ttattcgtct
     3601 tagaaaagtt cggaggtgct tgttgttgac ttgataaacc gaaaagcata cggctatttg
     3661 agaatatacc actgttacat gatgaagcag cagcggttaa tactacaaaa ttaatcaagc
     3721 ccgcagcaaa cggaattccg atcaatgcga ataattttac gaatggactg ttatcaggat
     3781 caacttgctg ccaagggata atagacatga taaccgctaa cgcaccaacg taaaatatta
     3841 aaattctaat gggtacactg ttaattgcct ttggaattgt tttaactgga tcttttgttt
     3901 ccccagctgt aacaccaatc atttcaattc ctacaaatga gaataatgcc atttggaaag
     3961 acataaagaa tccagaagca cctttagcga atatgccgtg ttcatataaa tttgtgaaac
     4021 tggcatgccc gaattgagtt ttaaatgcaa atagaatcat gacgaaacca actactatta
     4081 aaccaataat tgtcgctatt ttaatgatag agaaccaaaa ttctaattct ccgaaaagtc
     4141 ttgcgctaag taggttgaat gacattaata acagtacaca aaatagtgca cttatccagt
     4201 ttggaatttc tgggaaccaa aagctaacat attttgccac agccgttact tcagccatac
     4261 ctgtaataat ccaacagaac cagtatgtcc atccggtaac aaatcctgca aaaggcccaa
     4321 tatatgtatt ggttacatct gcgaaagatt taaattcagt attctgtata atgatttctc
     4381 ctaaacctcg cataaacata aataacataa atcctataat gatgtatgtt aatagaattg
     4441 aagggccggt taatgcaatc gtttgaccag cacctaagaa taagcctgta ccaattgcac
     4501 cgccaattgc aattaattgt atgtggcgat tgctcagttc cctttgtaat ttttcagcca
     4561 taatacatct cccttaaata tagatatgtt tattatgcac ttatattgag atatatacaa
     4621 ttattttcgg taaaaatgtg taaaattcca tgttaatata ctttggtttt tataatcata
     4681 tataataacc aattgaaaat ttaattctat tgtaaaattc atggattatt cacatcttga
     4741 aaaagcttta atggtgctat ttgtggctat tctgtgacat ttacatagat ttacaaaaaa
     4801 attgttgcac atataatgcc agtttttata tttcacaaac gaaatgcgtt tagctataat
     4861 attagttgaa agccatttca taaagaaaca gtaaagggga aatttatcat ggcagaatta
     4921 caaagaggtt tagaaggggt tatcgcagcg gagactaaaa taagttcaat tattgaaagt
     4981 caattgactt atgccggcta tgatattgat gatctagctg aaaatgcgca atttgaagaa
     5041 gttattttcc tattatggaa ctatagattg ccaaacgaag aagagcttgc tcatctaaaa
     5101 gggaaattaa atcaatacat gacattaaat cctcgtgtgt atacacattt tgaggagtat
     5161 gttacagatc acgtgcatcc aatgacagca ttacgtacgt cattatcata tattgcacat
     5221 ttcgatcctg atgctgaaaa tgaatcagat gaaaatcgtt atgaaagagc aatgcgtata
     5281 caggctaaag tagcatcatt agttacagcg tttgctcgag taagacaaga taaagaacca
     5341 cttaagccta atcctgactt aagttatgcg gcaaacttcc tatatatgtt acgtggggaa
     5401 ttaccaacag atatagaagt agaagccttc aataaagcac ttattttaca cgctgatcat
     5461 gagttgaacg catctgcatt tacagcacgt tgtgcggtat catcattgtc agatatgtac
     5521 tcaggtattg tagcagctgt aggttctctg aaagggccat tacatggtgg tgcaaacgaa
     5581 caagttatga cgatgttatc tgagattggg tcaattgaaa atgttgatgc ttacttagat
     5641 gaaaaatttg ctaataaaga taaagtaatg ggctttggtc atcgtgtata taaagatggt
     5701 gatcctagag cgaaatattt aagagaaatg agccgtcaaa ttacgaaaga cgctggtcgt
     5761 gaagaattat ttgaaatgtc agtgaaaatg gaaaaacgta tggcagaaga aaaaggatta
     5821 attcctaatg ttgattttta tagtgcgagt gtttatcact gtatggaaat acctcatgac
     5881 ttattcacgc caatctttgc tgtaagtcgt tctgcaggat ggattgctca tattttagaa
     5941 caatataaag ataatagaat tatgcgtcct agagcgaaat atattggcga aacgaatcgt
     6001 aagtatatcc cgcttgaaga aagaaaataa tcaatacaaa ttaaaaatga agatgtaaaa
     6061 tttggaggta aaataactat gactgcagaa aaaattactc aaggaactga aggattaaac
     6121 gtacctaatg aaccaattat cccatttatt atcggtgatg gaattggacc ggatatttgg
     6181 aaggcagcaa gccgagttat agatgctgct gttgagaaag cctataatgg cgaaaaacgc
     6241 attgaatgga aagaagtgct agctggccaa aaagcatttg atacaactgg tgaatggtta
     6301 cctcaagaaa cacttgatac aattaaagaa tatttaattg ctgttaaagg acctttaaca
     6361 acaccaattg gtggtggtat tagatcatta aatgtggctt tacgccaaga attagattta
     6421 tttacttgct taagaccggt acgttggttt aaaggagtac catcacctgt taaacgtcca
     6481 caagatgttg atatggttat tttccgtgaa aatactgaag acatttatgc tggtattgaa
     6541 tttaaagaag gtacaacaga agttaaaaag gtaattgact tcttacaaaa cgaaatgggt
     6601 gcgacaaaca ttcgattccc agaaacttca ggtattggta ttaaaccagt ttctaaagaa
     6661 ggaactgagc gattagttag agcagctata caatatgcta tcgataataa ccgtaaatca
     6721 gttactttag ttcataaagg taatattatg aaatttacag aaggctcatt taagcagtgg
     6781 ggttacgatt tagcattatc tgaatttggt gatcaagtat tcacttggca acaatatgac
     6841 gaaattgttg aaaatgaagg cagagatgct gctaatgctg ctcaagaaaa agctgaaaaa
     6901 gaaggcaaga ttatcattaa agattctatt gctgacattt tcttacaaca aattttaact
     6961 cgtccagctg agcatgatgt tgtagcaact atgaacttga atggtgacta tatttcagat
     7021 gctttagctg cacaagttgg tggtattggt attgcgccag gtgcaaacat taattatgaa
     7081 acaggtcatg ctatttttga agcaacacat ggtacagctc caaaatatgc aggtttaaat
     7141 aaagtgaatc catcttcagt aattttaagt tctgtattaa tgttagaaca tttaggatgg
     7201 caagaagcgg cagataagat tacagattca attgaagata caattgcttc aaaagttgtt
     7261 acttatgact ttgcccgttt aatggatggt gctgaagaag tttctacatc agcatttgca
     7321 gatgaattga ttaaaaattt aaaataagca aaataaaatt aggtgagtct agcaacttta
     7381 attgctaggt tcgccttttt aaatgaattg aagttggcaa caggatagaa ataataaaga
     7441 gtcaccaatg attgattatg tagtgtttat tacacattag acaaagcgaa tttgtacata
     7501 agcttaggaa taaatgagta aaactcatgc ataggtagtg ggatagaaat gatattttta
     7561 caaaatttat ttcgttgtcc cagagtaagt catgcaaatg atagaaggaa atataagaag
     7621 tattttattt atatgtaaag taatgtaaaa aatgtgtaaa gataatgtgt aggatgttat
     7681 ttttataaat agttcggtta taatgtaaat ataattataa gttagggagg cataccatgt
     7741 cgcaaaaagt gttggtagta gatgacgaac attcaatcgt aacattactt aaatataact
     7801 tagaaacagc tggttatgaa gttgttgtcg catttgatgg tgatgaggct ttagaaaagg
     7861 tagaaagtga acagccagat ttaattattt tagatgttat gctacctaaa aaagatggca
     7921 ttgacgtatg taagactgta agaacaaata aaaatttagt ccctatatta atgttaacgg
     7981 cgaaaaatga tgagtttgat cgggtattag gtttagaatt aggtgccgat gactatatga
     8041 ctaagccttt ttcacctaga gaagttgttg cccgtgtgaa agccatttta agacgttctc
     8101 aatttgtaaa agaagtagaa gagattgacg atgatattac catcggatcg atacgtatta
     8161 gacccgatta ttttgaagta tataagcata atgagttatt ggagctaaca ccgaaagaat
     8221 ttgaactctt gctttatttg atagaaagac aaggcagagt cattacgaga gagcatatgt
     8281 taaatacggt atggaattat gaatttgcag gagactcaag aattgttgat gtacatatca
     8341 gtcatttaag agataaatta gaagataatc ctaagaagcc acaacttatt aaaactgtgc
     8401 gtggcttagg ctataaattg gaaagaccta aagaacaatg atgaagtttc accaccgctt
     8461 aatgttattg ataagcagta tattaattat cagttttgtt acattaggca ttattattca
     8521 cgcttccatc acatatgttt tatcagaaaa acacgaacaa gctctagcaa cagatgcaag
     8581 atcatatatt tatttagtcc aagataatga gattgataaa gttaaagaaa ttgtaaaaca
     8641 gcaaaatata gatttattga taactaaaca tgataaaaag gtgtttagta gtggcaaact
     8701 gaaagctatc ccaaatagtg atagctatat tcaaaaaagg gacgttttta tttttaataa
     8761 gaaggttaac ggttatcatg tatggattaa aggttacaat aacgatatta cagaaatgca
     8821 ttggacgttg tggaaatatc ttatattgac atgtttagtt gtactcattt gtctgtattt
     8881 tgcaagccgt agttttaagc gtacattaat ccgtcccatt caagaagtta cttatgcaac
     8941 acaattgttg gcaaatggtt attatcacat acgtgtacct gaaagtaatg ttgtagaaac
     9001 gaaagcgctt tttgtgtcta ccaatgattt agcacgacgc cttcaaaagc ttaataatga
     9061 gcaaaaaatt caatccaacc gtttaaaaac aacgattgaa aatattccaa gtgctatttt
     9121 aatgatagat cgaaatggaa aaatcgttgt tgcaaataaa gcgtattatg aacagttcaa
     9181 catctcgcat aatattgaac aagtaggcta tcacggctat gttaatactg aaattgagca
     9241 attaatttta gaaagtttta aagttgaaaa acctatttat gaacaattgg aagttgcaat
     9301 aaaccaagtc catgccaagt actttgatat ttcatgcgtt ccgattttaa ctaggtctca
     9361 aaaaagtttg caagggattt tagttgtgat gcatgatata acaaatctga aacaacttga
     9421 aaatctaaga cgtgaatttg tagcaaatgt ttcacatgaa ttaaaaacac ctattacttc
     9481 aattaagggg tttgctgaaa cgcttattga tggtgcaaaa aatgatgcgg aatcattaga
     9541 catgttttta aacataattt taaaagaatc aaatcgaata gaatctttag taacagatct
     9601 gttagattta tcgcatattg agcaacatac agagttagat acagactata tgaatttatc
     9661 agatttaacg cggcgtatta ttgataatat gatgactcaa gccaatcaaa aaaatatttc
     9721 cattcatact gatattgaaa aagatgtcat tgttaaagca caagaaagta aaattgctca
     9781 agttatcacg aatttgttaa cgaatgcaat taactattct tatgaagatg gagatattaa
     9841 cgttcgtgtg tatcgagatg actttcgtgt cattttcgaa gtacaagatt ttggtatagg
     9901 tattaaatta gaagaccaac aacgtatttt tgaaagattt tatagagtag ataaagcacg
     9961 aagtagagat tctggtggga caggtcttgg attatcaatt acaaagcata tagtagaggc
    10021 ccaccaaggc aatattgaag tgaatagtca agttggcaaa ggctcaacgt tcaaagttat
    10081 tctaaaagat tataaagaat aaaaattaaa gtggtaacag cgcgtgtatt tttcacgaag
    10141 ttgttgctgc ttttttattt cgttcaaatc tctattggta ttaaattaga gttaatgaat
    10201 tggaataaat tcggttgtca attttgtcat tagcatatat cgt
//
LOCUS       DANBWH010000092        10240 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.095, whole genome shotgun sequence.
ACCESSION   DANBWH010000092 DANBWH010000000
VERSION     DANBWH010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10240
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.095"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..218)
                     /locus_tag="PPY92_001561"
     CDS             complement(<1..218)
                     /locus_tag="PPY92_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625566.1"
                     /translation="MTMTDSGRKEYLKHFFGSKRYLYQDNERVAHIHVVNDTYYFHGH
                     IVPGWQGVKKTFDTAEELETYIKQHDLE"
     gene            complement(218..472)
                     /locus_tag="PPY92_001562"
     CDS             complement(218..472)
                     /locus_tag="PPY92_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HDJ2625567.1"
                     /translation="MSIISNRKVDMNKTQDNVKQPAHYTYGDIEIIDFIEQVTAQYPP
                     QLAFAIGNAIKYLSRAPLKNGHEDLAKAKFYVDRVFDLWE"
     gene            complement(472..873)
                     /locus_tag="PPY92_001563"
     CDS             complement(472..873)
                     /locus_tag="PPY92_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVL family protein"
                     /protein_id="HDJ2625568.1"
                     /translation="MARRKVIRVRIKGKLMTLREVSEKYHISPELLRYRYKHKMRGDE
                     LLCGRKDSKSKDEVEYMKSQIKDEEKEREKIRKKAILNLYQRNVRAEYEEERKRRLRP
                     WLYDGTPQKHSRDPYWFDVTYNQMFKKWSEA"
     gene            complement(873..1058)
                     /locus_tag="PPY92_001564"
     CDS             complement(873..1058)
                     /locus_tag="PPY92_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3113 family protein"
                     /protein_id="HDJ2625569.1"
                     /translation="MQHQAYINAPVDIRIPTEVESVNYNQIDKEKENLADYLFNNPGE
                     LLKYNVINIKVLDLEVE"
     gene            complement(1071..3023)
                     /locus_tag="PPY92_001565"
     CDS             complement(1071..3023)
                     /locus_tag="PPY92_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HDJ2625570.1"
                     /translation="MNIDIETYSSNDISKCGAYKYTEAEDFEILIIAYSIDGGAISAI
                     DMTKVDNEPFHADFETFKIALFDPAVKKYAFNANFERTCLAKHFNKQMPPEEWICTMV
                     NSMRIGLPASLDKVGEVLRLQNQKDKAGKNLIRYFSIPCKPTKVNGGRTRNLPEHDLE
                     KWQQFIDYCIRDVEVEMTIAHKIKDFPVTAIEQAYWVFDQHINDRGIKLSKSLMLGAN
                     VLDKQSKEELLNQAKHITGLENPNSPTQLLAWLKDDQGLDIPNLQKKTVQEYLKEATG
                     KAKKMLEIRLQMSKTSVKKYNKMHDMMCSDERVRGLFQFYGAGTGRWAGRGVQLQNLT
                     KHYISDTELEIARDLIKEQRFDDLDLLLNVHPQDLLSQLVRTTFTAEEGNELAVSDFS
                     AIEARVIAWYAKEQWRLDVFNTHGKIYEASASQMFNVPVESITKGDPLRQKGKVSELA
                     LGYQGGAGALKAMGALEMGIEENELQGLVDSWRNANPNIVNFWKACQEAAINTVKSRK
                     THHTHGLRFYMKKGFLMIELPSGRALAYPKASVGENSWGSQVVEFMGLDLNRKWSKLK
                     TYGGKLVENIVQATARDLLAISIARLEASGFKIVGHVHDEVIVEIPRGSNGLKEIETI
                     MNKPVDWAKGLNLNSDGFTSPFYMKD"
     gene            complement(3091..3648)
                     /locus_tag="PPY92_001566"
     CDS             complement(3091..3648)
                     /locus_tag="PPY92_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HDJ2625571.1"
                     /translation="MKAKVLNKTKVITGKVRASYAHIFEPHSMQEGQESKYSISLIIP
                     KSDTSTIKAIEQAIEAAKEEGKVSKFGGKVPANLKLPLRDGDTEREDDVNYQDAYFIN
                     ASSKQAPGIIDQNKIRLTDSGTVVSGDYIRASINLFPFNTNGNKGIAVGLNNIQLVEK
                     GEPLGGASAAEDDFDELDTDDEDFL"
     gene            complement(3674..4840)
                     /locus_tag="PPY92_001567"
     CDS             complement(3674..4840)
                     /locus_tag="PPY92_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HDJ2625572.1"
                     /translation="MKLDHSNRAHAKLSASGAKQWLNCPPSIKASEGIADKSTVFAEE
                     GTFAHELSELYFSLKYEGLTQFEFNKAFQNYKRNQYYSEELREYVEEYVANVEEKYNE
                     ALSRDNDVIALFETKLDLGKYVPESFGTGDVIIFSGGVLEIIDLKYGKGIEVSAIDNP
                     QLRLYGLGAYELLSLMYDIHTIRMTIIQPRIDNFSTEELPISRLLQWGADFVKPLARL
                     AYNGGGEFKAGSHCRFCKINHSCRTRAEYMQNVPQKPPHLLSDEEIAELLYKLPDIKK
                     WADEVEQYALNQAKENDKNYPGWKLVEGRSRRMITDTKAMLEKLVEAGYKPEDITETK
                     LLSITNLEKLIGKKAFSKITEGFIEKPQGKLTLATESDKRPAIKQSAEDDFDKL"
     gene            complement(4837..5199)
                     /locus_tag="PPY92_001568"
     CDS             complement(4837..5199)
                     /locus_tag="PPY92_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625573.1"
                     /translation="MAILEDIFEELKLLNKNLRVLNTELSTVDSSIVQEKVKEAPMPK
                     EETAQLESIEEVKETSADLTKDYVLSVGKEFLKKADTSDKKEFRNKLNELGADKLSTI
                     KEEYYEKIVDFMKARINA"
     gene            complement(5214..5537)
                     /locus_tag="PPY92_001569"
     CDS             complement(5214..5537)
                     /locus_tag="PPY92_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HDJ2625574.1"
                     /translation="MTLQQKILSHFATYDNFNSDDVVETFGISKTHAKSTLSKLKKKG
                     KIEMESWGIWRVIESQLHLSVVERKKEILEEQFELLARLNEQSEDPREIEERIKLMIR
                     LANQF"
     gene            complement(5616..5777)
                     /locus_tag="PPY92_001570"
     CDS             complement(5616..5777)
                     /locus_tag="PPY92_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
                     /protein_id="HDJ2625575.1"
                     /translation="MSDTYKSYLIAVLCFTVLAIVLMPFLYFTTAWSIAGFASIATFI
                     FYKEYFYEE"
     gene            complement(5790..6053)
                     /locus_tag="PPY92_001571"
     CDS             complement(5790..6053)
                     /locus_tag="PPY92_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDJ2625576.1"
                     /translation="MPKIIVPPTPENTYRGEEKFVKKLYATPTQIHQLFGVCRSTVYN
                     WLKDYREDNLGVENLYIDYSPTGTLINISKLEEYLIRKHKKWY"
     gene            complement(6080..6295)
                     /locus_tag="PPY92_001572"
     CDS             complement(6080..6295)
                     /locus_tag="PPY92_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625577.1"
                     /translation="MNTRSEGLRIGVPQVSSKADASSSYLTEKERNLGAEILELIKKS
                     DYSYLEINKVFYALDRELQYRANNNKL"
     gene            6350..6715
                     /locus_tag="PPY92_001573"
     CDS             6350..6715
                     /locus_tag="PPY92_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625578.1"
                     /translation="MPLISDEFDTLTKDQQYILSVLYKDYLECVKLGSVKLTCNNFGS
                     AKDIHTKYFQKLHFEDVKYDLNKLKNSGFLNGVYASNTIYHVTISDKTVVYFENEFKN
                     NLKSIIDSISKIASIIPGL"
     gene            complement(6684..6929)
                     /locus_tag="PPY92_001574"
     CDS             complement(6684..6929)
                     /locus_tag="PPY92_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HDJ2625579.1"
                     /translation="MNIQEATKIATKNLVSMTRKDWKESHRTKILPTNDSFLQCIISN
                     SDGTNLIRYWQPSADDLMANDWEVINPTRDQELLKQF"
     gene            complement(6958..7734)
                     /locus_tag="PPY92_001575"
     CDS             complement(6958..7734)
                     /locus_tag="PPY92_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HDJ2625580.1"
                     /translation="MQALQIVEQNETHYVDSREVAEMIGKRHDNLVRDIKGYIKVLED
                     SSKLSSHNFFEESTYVNSQNKVQPCYLLTKKGCDIVANKMTGSKGILFTATYVDAFHK
                     MDEYIKQQAQLNVPQTPMQALEMMFKAQKDQEQFNKQMQQEITGIRHIVGIETKNWRN
                     DTNKMLSAIAQHLGGGAMHQKVKSEAYKALEEKGRCNLKIRMQNRKGKMLANGATKTQ
                     INKLSKLDVITDEPRLVEIYISVIKSMAIKYGVDISQFEI"
     gene            complement(7748..7984)
                     /locus_tag="PPY92_001576"
     CDS             complement(7748..7984)
                     /locus_tag="PPY92_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002469095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDJ2625581.1"
                     /translation="MPIDAKLLKSKMALKEHNIKTLSEEIGVNRDTLSNMIHGRTKPS
                     YPVINGIYFALELTPQEGRDIFFNEDLRKKKVLT"
     gene            8136..8450
                     /locus_tag="PPY92_001577"
     CDS             8136..8450
                     /locus_tag="PPY92_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDJ2625582.1"
                     /translation="MCFSKRMKQSREKQGMTLAELGRKIGKTEATVQRYESGNIKNLK
                     NDTIESIATALNVNPAYLMGWVEENDDEVQHRAAHLEGELTDDEWQRVLDYANYIRSK
                     RK"
     gene            8463..8924
                     /locus_tag="PPY92_001578"
     CDS             8463..8924
                     /locus_tag="PPY92_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000525002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDJ2625583.1"
                     /translation="MGLYEETLIQHDYIEIREADVLPDNLDGVWLGDLILIKRGLSDR
                     EKAGILFEELAHNKLTYGDIADYSKFNNRKFENYARRHGFISAVPLREIVEAYNYGVR
                     NLYELSEYLQLSEEYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI"
     gene            8942..9526
                     /locus_tag="PPY92_001579"
     CDS             8942..9526
                     /locus_tag="PPY92_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001636973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625584.1"
                     /translation="MKRLLGLTLASALVLGACGSHDGDKKEESKKTETKKDNKDKKKE
                     TKEKAEAKKENANQNDNNNQVNNESNTNVNNDQQTNRPLTKDEISQRVKNGHNVNGMV
                     DADGNTWYQAQGAGDVIGYTKPDGTQCTVGGCVTPQQQEQINEANYKEMEKYGYSREK
                     YDAIQKEASKLQQQKENGEITAEEFTNRYIELYD"
     gene            9756..9902
                     /locus_tag="PPY92_001580"
     CDS             9756..9902
                     /locus_tag="PPY92_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625585.1"
                     /translation="MNGGENFMVDKNKKQETTRSNPLNKSFEKSGASEKLKSTLSEKA
                     KKKD"
     gene            complement(9899..>10240)
                     /locus_tag="PPY92_001581"
     CDS             complement(9899..>10240)
                     /locus_tag="PPY92_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDJ2625586.1"
                     /translation="FNPLIVKFIIWLINITRKFMKLDCISLLDKRDKLFNNNGKPVFI
                     VIKDFENRIIEEGELKTYNSAGSDFDLLEVERQDFKVSDLPSNDELYIKHTLVDLKQQ
                     IKLDLYLMNEY"
BASE COUNT         3127 a         1879 c         1549 g         3685 t
ORIGIN      
        1 tattccaaat catgttgctt tatatatgtt tcaagctctt cggctgtatc aaatgtcttt
       61 ttcacgcctt gccaacctgg tacgatatgc ccatgaaagt aataagtgtc atttactaca
      121 tggatatgtg ccactcgctc gttatcctga tacagatatc tcttagagcc gaaaaaatgt
      181 tttaagtatt ctttacgtcc gctatctgtc atggtcatca ctcccacaag tcaaatactc
      241 tatcgacgta aaacttcgcc tttgctaaat cctcatgacc attctttaac ggtgctctag
      301 acaagtattt aattgcatta cctattgcga atgctaattg tggtgggtac tgtgccgtaa
      361 cttgttcaat aaaatctata atttcaatgt cgccgtatgt gtaatgcgca ggttgcttaa
      421 cgttgtcttg cgttttgttc atatctactt ttctgttact gattatgctc attatgcttc
      481 actccatttc ttgaacattt ggttataagt gacatcgaac cagtacggat cacgtgaatg
      541 tttttgaggc gttccatcat aaagccatgg tctcaatctt ctctttcttt cttcttcata
      601 ttccgctctc acatttcgtt ggtataggtt caaaatcgct ttttttctga ttttttctct
      661 ctctttttct tcatctttta tttgactctt catatattca acttcatctt tagattttga
      721 gtcttttctt ccacacaata attcatcgcc gcgcatttta tgtttgtatc tgtatctaag
      781 aagttctgga gatatatgat atttttctga aacttctctc aatgtcatta gttttccttt
      841 gatacgcact cttataactt ttcttctagc catcattcca cctctaaatc taaaaccttg
      901 atatttataa cgttatattt taatagttca cctggattat taaataaata gtccgccaaa
      961 ttctcttttt ctttatcaat ctgattgtaa ttaacacttt cgacttctgt aggaattcta
     1021 atgtcaacag gagcattgat ataagcttga tgttgcattc aatcacactc ctaatccttc
     1081 atataaaacg gagaagtaaa tccgtcacta ttcaaattca atccttttgc ccaatcgaca
     1141 ggcttattca tgatagtttc gatttcctta agtccatttg aacctctagg tatttctaca
     1201 attacttcat catggacatg tccaactatt ttaaaacctg atgcttcaag cctagctata
     1261 gaaatcgcaa gtaaatccct tgcagttgct tgaacaatat tctcgactaa cttcccacca
     1321 tacgttttta actttgacca tttacggtta agatctaagc ccataaattc aacaacttga
     1381 ctaccccaac tattttcacc aactgaagct tttggataag ctaaagctct tccactaggc
     1441 agttcaatca ttagaaaacc ttttttcata taaaatctaa gtccatgcgt atgatgcgtc
     1501 tttcgggatt tcacagtatt aattgcagcc tcttggcaag ccttccaaaa attaactatg
     1561 ttaggatttg cgttacgcca actatcaact aaaccttgta attcattttc ttcaatgccc
     1621 atttccaatg cacccatcgc ttttaaagct ccagcgccac cttgataacc taaagctaat
     1681 tcggacactt ttcccttttg tctgagaggg tcgcctttag ttatgctttc taccgggaca
     1741 ttaaacattt gagaagccga tgcttcatat atctttccgt gtgtgttgaa cacatctaaa
     1801 cgccattgtt cttttgcata ccatgctatg actcttgcct ctattgcaga aaaatcactt
     1861 actgctagtt cattaccttc ttcagcagta aatgtcgtcc taactaattg acttaataag
     1921 tcttgaggat gaacattgag taataaatct aaatcgtcaa aacgttgttc tttaataaga
     1981 tctcttgcta tttctaattc agtatctgaa atataatgct ttgttaaatt ctgaagttgt
     2041 acacctctac ctgcccatct tccagtaccg gcaccgtaaa attgaaacag acctcttacc
     2101 cgttcatcac tgcacatcat gtcatgcatt ttgttgtatt ttttcacact ggttttagac
     2161 atttgcaatc taatttctag cattttttta gcttttcctg ttgcttcttt taagtactcc
     2221 tgaaccgttt tcttttgtaa attaggtata tctaatcctt ggtcatcctt taaccaagcc
     2281 aataattgtg taggactatt aggattttct aaacctgtta tatgtttagc ttgattaagc
     2341 aattcttctt tactctgctt atcgagcaca ttagctccta acatcaatga tttagaaagc
     2401 ttaatacctc tgtcgtttat atgttggtca aaaacccaat atgcttgttc aattgcagtt
     2461 actggaaagt ctttaatttt atgagcaatc gtcatttcta cttctacatc tcgaatacag
     2521 taatctataa attgttgcca tttttcaaga tcatgttcag gcaagtttct tgttcttcct
     2581 ccattaactt ttgttggttt acaaggtata gagaaataac gaattaaatt tttacctgct
     2641 ttatcttttt ggttttgtag tcttaaaact tctccaactt tatcaagcga agcaggtaag
     2701 ccaatacgca ttgaattaac cattgtgcaa atccattctt caggtggcat ctgtttatta
     2761 aaatgtttag caagacaagt tctttcgaaa ttagcattga atgcatactt ttttacagca
     2821 ggatcaaaaa gagcaatttt aaacgtctca aaatcagcgt ggaaaggctc attatctact
     2881 ttagtcatgt caatcgcact aatcgctcca ccatctattg aataagctat aattaaaatt
     2941 tcgaaatctt cagcttctgt gtatttatag gcaccacatt tcgaaatatc attactgcta
     3001 tatgtttcaa tatctatatt cataaatctc aaattcttga cacctcaatt tctttaaaat
     3061 taaagtgggg ctaaaacccc acctattgac ttataagaaa tcctcatcat cagtgtctaa
     3121 ttcatcgaaa tcatcttctg ctgcacttgc accgccaaga ggttcgcctt tttctacaag
     3181 ttgaatgttg ttcaatccaa ctgcgatacc cttattacca tttgtgttga aaggaaatag
     3241 attaattgaa gctctaatat aatcaccact tacaacagtt ccagaatccg ttaatctaat
     3301 tttgttttgg tcaataatac caggtgcttg tttgcttgat gcgttaataa aataagcgtc
     3361 ttgataattg acatcatctt ctctttcagt atctccatca cgtaatggaa gtttcagatt
     3421 tgcaggaact ttgcctccaa acttactaac ttttccttct tctttagcag cttctatagc
     3481 ttgttcaatg gcttttatcg tacttgtatc tgatttagga atgattaaac tgattgaata
     3541 ctttgattct tgcccttctt gcatactgtg aggttcaaaa atatgtgcat atgatgctct
     3601 tacttttcct gtaatcactt tagttttatt taatactttt gctttcatgt ttatataccg
     3661 tccttttaaa tttttatagt ttgtcaaaat catcttcagc agattgcttt atagctggtc
     3721 gtttatccga ctcggtagca agtgttaatt taccttgcgg cttttctata aaaccctctg
     3781 taattttaga aaatgctttt ttaccaatta atttttctaa attcgtaatg ctaagtaact
     3841 tggtttctgt aatatcttca ggtttataac ccgcttcaac taacttttca agcattgctt
     3901 ttgtatcagt tatcattctt cgtgaacgac cttctacaag cttccaacca ggatagtttt
     3961 tatcattttc tttcgcttga tttagtgcat attgttctac ttcatcagcc cattttttga
     4021 tatcaggcag tttatataaa agttctgcaa tctcttcatc acttaacaaa tgtggtggct
     4081 tttgaggcac attttgcatg tattctgcac gtgttctaca tgaatgattt atcttacaga
     4141 atctacaatg actacctgct ttaaactctc ctccaccgtt ataagcaagt ctggctaatg
     4201 gtttaacaaa atcggctccc cattgaagta atcttgatat tggtaactct tcagtagaaa
     4261 agttatctat tcgaggttgt atgatagtca tgcgaattgt atgaatgtca tacattaaac
     4321 taagcagttc atatgcgccc aagccatata atctaagttg aggattatct atagctgaaa
     4381 cttcaatgcc tttaccatat ttaaggtcaa taatttcaag tacaccacct gaaaatataa
     4441 tgacatcacc agttccaaaa gattcaggga cgtatttacc taaatctaat tttgtttcaa
     4501 ataaagctat tacatcatta tccctactca aagcttcgtt atatttttct tctacattag
     4561 ctacatactc ttccacatat tcacgcaatt cttcgctgta atattgattt ctcttataat
     4621 tttgaaaagc cttattaaac tcaaactgtg ttaggccttc atatttaagg ctgaaatata
     4681 actcacttaa ctcatgggcg aatgtacctt cttcggcaaa aactgtactt ttatctgcaa
     4741 taccttcact tgccttaata ctcggtgggc agtttagcca ttgttttgct ccacttgcac
     4801 taagctttgc atgagctcta tttgagtgat ctagcttcat gcattgattc tcgccttcat
     4861 aaaatcaaca attttttcat aatactcttc tttgatagta gatagcttat ccgcaccaag
     4921 ttcgttaagt ttatttctaa attctttctt atcagaagta tctgcttttt taaggaactc
     4981 ttttcctact gataaaacat aatctttagt caaatcagca gaagtttcct taacttcttc
     5041 aattgattcc agttgagctg tttcttcttt tggcattggt gcttctttaa ctttctcttg
     5101 tacaattgat gaatctacag ttgatagttc agtgtttaac acacgtaaat tcttatttaa
     5161 tagttttaat tcttcaaaaa tatcttctaa tattgccatt gattaactcc tccttaaaat
     5221 tggttagcta gacgaatcat taacttgata cgttcttcta tttctctagg gtcctcactt
     5281 tgttcattta atcttgctaa caattcaaat tgttcttcta aaatttcttt tttacgttcg
     5341 actacactta aatgcaattg cgattcaata acacgccaga taccccaact ttccatttca
     5401 atctttcctt ttttcttaag ttttgaaaga gtggattttg catgtgtttt agatatccca
     5461 aaagtttcaa caacatcatc agaattgaaa ttgtcatatg ttgcaaaatg tgatagtatt
     5521 ttttgttgta aggtcatatt aataactcct tatataatta tttaagacaa atgcttatct
     5581 ttaactgtta cttgttgtcg caagtagcag tttttttatt cttcataaaa gtactcttta
     5641 tagaatatga atgttgcgat acttgcgaat cctgcaattg accacgctgt agtgaagtat
     5701 agaaacggca tgagtacaat cgctaagacc gtgaagcata gcactgctat taggtagctt
     5761 ttatatgtgt cgctcatttg ataatcctcc taataccatt ttttatgctt tctgatcaaa
     5821 tactcttcca atttagaaat attaatcagt gtgcctgttg gtgaataatc aatgtataaa
     5881 ttttctacac ctaaattatc ttcacggtaa tctttcaacc agttgtatac tgtgcttcta
     5941 catactccaa acaattgatg gatttgcgta ggtgttgcgt ataacttttt aacaaatttt
     6001 tcttcgcctc tatatgtgtt ttctggtgtt ggtggtacta tgatttttgg catttctatc
     6061 tttccttccg tgtataatgt taaagtttgt tattattcgc cctgtattga agttctctat
     6121 ctaatgcata gaaaactttg tttatttcta agtagctgta atcacttttt ttaataagct
     6181 ctaatatttc cgctcctaag ttacgttcct tttccgttaa ataggatgaa gaagcatcag
     6241 ctttgctaga aacttgtggg acgcctatac gcaatccttc tgatcttgtg ttcatttgtt
     6301 tatgctcctt tcgtgtataa tgttgttatc aacctaagga ggtgataaca tgcccttgat
     6361 atctgatgaa tttgatacac ttactaaaga ccaacaatat atcttgtccg tactctacaa
     6421 agattattta gaatgtgtaa agttaggttc ggttaaatta acctgcaata attttggaag
     6481 tgctaaagat atacatacaa agtattttca aaaactacat ttcgaagatg taaaatacga
     6541 tttaaataaa cttaaaaact ctgggttcct aaacggcgtg tatgctagta acactattta
     6601 tcatgtaaca atttcagaca agactgttgt ttactttgaa aatgagttta aaaacaattt
     6661 aaaaagtatc attgatagca tttctaaaat tgcttcaata attcctggtc tctagttggg
     6721 tttataactt cccaatcatt tgccatgagg tcatcggctg aaggttgcca atatctgata
     6781 aggtttgtcc catcgctatt tgaaatgatg cattgtaaaa aactatcatt tgttggtaat
     6841 atcttagttc gatgactctc tttccaatct ttccgtgtca tagagacaag attttttgta
     6901 gctatcttag ttgcttcttg aatgttcatt tgttattcct ccttttaaga tgtttgttta
     6961 aatttcaaat tggctaatat ctacaccgta tttaatcgcc atactcttaa tcactgaaat
     7021 gtatatctca accaatctag gttcatcagt aatcacatct aattttgaca acttgttaat
     7081 ctgtgttttc gttgcaccat tcgctagcat tttacctttg cgattctgca tacggatttt
     7141 taaattacaa cgtccttttt cttctaaagc tttatatgct tcagacttaa ctttctggtg
     7201 cattgctccg ccacctaaat gttgtgcaat cgcagataac attttgtttg tgtcgttacg
     7261 ccagtttttc gtttcaatac cgacaatgtg acgaatgcct gtgatttctt gttgcatttg
     7321 tttgttaaac tgttcttggt ctttttgtgc tttgaacatc atctctaatg cttgcattgg
     7381 tgtttgtggt acattaagct gtgcttgttg tttaatgtat tcatccattt tatgaaatgc
     7441 atcaacataa gttgcagtaa acaaaatgcc tttactacct gtcatcttgt ttgctactat
     7501 gtcgcaacct tttttggtta gtaggtaaca aggttgtact ttgttttgtg aattaacata
     7561 ggtgctttct tcaaagaaat tatgactact caattttgag gagtcctcta aaaccttgat
     7621 ataaccttta atgtctctta ctaaattgtc gtgtcgcttt cctatcattt ccgcaacttc
     7681 tctactgtct acataatgtg tttcgttctg ttctactatt tgtaatgctt gcatttcagt
     7741 ttcctcctta agttaaaact ttctttttgc gtaagtcttc gttaaaaaaa atatctcttc
     7801 cttcttgagg tgtcaattct aacgcaaaat aaataccatt tattaccggg tacgacggtt
     7861 tcgttctccc gtgaatcata ttagataaag tatctctatt aacaccaatt tcttcagaaa
     7921 gggttttgat gttatgttct ttcaaagcca ttttagattt caaaagttta gcatctatag
     7981 gcatttcttt tcaccacctt tcgtattacg taagtaatct tatcatgatg ttacgaaaga
     8041 ggtcaagcac tttacgaaag ttttttagaa aaatattgca aatgccgaaa gttttcctta
     8101 taatagaact atcaagtaaa aggagctgta ttacgatgtg cttttcaaaa agaatgaaac
     8161 aatcaagaga aaaacaaggt atgactttgg ccgaactagg aagaaaaatt ggtaaaactg
     8221 aagctactgt acagcgttat gaaagcggaa atatcaaaaa tttaaaaaac gatactatag
     8281 aaagtatagc tactgcatta aatgttaatc ctgcatattt aatggggtgg gttgaagaaa
     8341 acgatgatga agtacaacat cgtgcagctc accttgaagg agaattgaca gatgatgaat
     8401 ggcaaagagt tttagattat gctaattata taagaagcaa acgtaagtaa aggatgtatc
     8461 agatgggatt atatgaagaa actttaatac aacatgatta tattgaaata agagaggctg
     8521 atgtgcttcc agataatttg gatggggtat ggttaggaga tttaatttta ataaagcgtg
     8581 gcttatcaga tagagaaaag gcaggaattc tctttgaaga attagcacat aataaactta
     8641 catacggtga tatagccgat tactcgaaat tcaacaatcg caagttcgaa aattacgcaa
     8701 ggcgacacgg ctttatctca gcagtcccgt tacgcgaaat tgtggaagct tacaattatg
     8761 gtgtacgtaa cttgtatgag ttgtctgagt atctgcaatt gagtgaagaa tacatattag
     8821 aagcaataga acaatataaa aagatatatg gtattgggac tcactacggc gaatactcaa
     8881 ttacatttga gccattgaga gtttttaaat ataaagaaat ataaacaaag gagaaattaa
     8941 aatgaaaaga ttattaggtt taacattagc gagtgcatta gttttaggcg cttgtggtag
     9001 ccacgacggc gataagaaag aggaaagcaa aaaaactgaa acaaagaaag ataacaaaga
     9061 taaaaagaaa gaaactaaag aaaaagcaga agcgaaaaaa gaaaatgcta atcaaaacga
     9121 taacaataat caagtaaaca acgagagcaa cacaaacgtt aacaacgatc aacaaaccaa
     9181 tagaccttta actaaagacg aaatatcaca aagagtaaaa aatggtcaca atgttaacgg
     9241 catggtagat gcagatggta atacttggta ccaagcacaa ggcgcaggtg acgttatagg
     9301 ttacacaaaa cctgatggta cacaatgcac agttggtggt tgtgtcacac ctcagcaaca
     9361 agaacaaata aacgaagcta attataaaga gatggaaaaa tatgggtatt ctcgtgaaaa
     9421 atacgatgca attcaaaaag aagcttctaa acttcaacaa caaaaagaaa atggagaaat
     9481 aacagcagaa gaatttacta ataggtatat agaattatac gactaagtat cttacaatca
     9541 attaattgta ttgtgattaa taacgtctat ttagtgattt aatataaata taaacaaagg
     9601 agaaattgac atgaaaaaag caatcttaac tttaagtctt atatttatta cctactacct
     9661 cacttttaaa tatatgtgga ttaaagaatt gaagtattaa cagcttttta tagcccttta
     9721 atataaaaat caaaaaacgc ctactagtgt agacgttgaa tggtggtgag aattttatgg
     9781 tagataaaaa caaaaaacaa gaaactactc gtagtaaccc attaaacaaa agttttgaaa
     9841 agtcaggcgc cagcgaaaaa ttaaaaagca ctttatcaga aaaagctaag aaaaaagatt
     9901 agtattcatt cattaaatat aaatccaatt taatttgttg tttaaggtct acaagcgtat
     9961 gtttaatata caattcatcg tttgacggta aatcagatac tttgaaatct tgtcgctcaa
    10021 cttctagtaa atcgaaatcg ctaccagctg aattataggt tttaagttca ccctcttcaa
    10081 tgattctgtt ttcaaagtct tttataacta taaatactgg tttaccgttg ttattaaaca
    10141 acttgtctct tttgtctaat aagcttatac aatccaattt cataaacttt ctggttatat
    10201 taattaacca gataataaat ttaacaatta aaggattaaa
//
LOCUS       DANBWH010000093        10204 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.096, whole genome shotgun sequence.
ACCESSION   DANBWH010000093 DANBWH010000000
VERSION     DANBWH010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10204
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.096"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..1474
                     /locus_tag="PPY92_001582"
     CDS             <1..1474
                     /locus_tag="PPY92_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625587.1"
                     /translation="IGGIAWGVYAFFANTPKNTYLKSEQQTAKMYKDYFNDRFENEVK
                     FQEKMKDNSFLSSLELSADASDEIVKGLGIPKSVVNASKIKMSYGHDPKKEKSMINLE
                     PTIADSALGKFQLAADKDKHYFESPLFKGKYSVNNSDLLSTYSKLTGEDEETAKENGI
                     TNQQLNLNTLFSNAQAQQSDYSKIAEKYSELIVDKLDDDNFDKGKKEEIKVNGEKYKV
                     RPVTLTLSRADTKKITLAVLEEAKKDKDLKKLMEEQGATKDYDKDIKKAIDDVKETKK
                     DEFAKIQSKIYTEKHTIVKREITITDKENNKTKIKGTNTLEDDKLKLDYALDFDQDKY
                     TYAEAKYTIKGVSSKEKDNKYSDKYEFGKKTEYDESKIKLDNQEKVDGTKRQDKGKIT
                     VALDKYSDENEFTFENNIDSDVKNNTQKSTLNIGIKYAEEPINFILKSSTKLKADIDF
                     DDSGAKDFNSLSSKDREKLEKEIEKNGGKMFESILKKASK"
     gene            1542..2708
                     /locus_tag="PPY92_001583"
     CDS             1542..2708
                     /locus_tag="PPY92_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDJ2625588.1"
                     /translation="MKTIHLFRIYHSFLLKKWYLIIYLLFILAALLITLTTIQHVTED
                     DNHFNIGVVDKDQSSETKLILNSIGKGSNLGKNVSIKAYDDKQAHTLLKKHKLQGYFV
                     FDKGMTKAFYKQGELPISVYTYDQQSMKSVVLSQLTDSVYQRLMRSMGGILAFQDLAP
                     KASHSDSINVMTDLLITGLNRSGAFNLEPIHLYDTGSYYAITGFLATVFIFALSLFTV
                     LKMNQDTVLKARLKMFHFSKERLLIIRALITWFYTMLWSIVGVVWIVFSIPNTFELYN
                     WPTLAIHLSYYVTFLILWLLLIELLTTGLLNSISKVILAIVILVLSGLTIPTIFLQHI
                     ANGVFNIQPFAVVTNQLLEIILNNYILELHPSFYLSFIALLIINLAVLVWRYRQ"
     gene            2705..3625
                     /locus_tag="PPY92_001584"
     CDS             2705..3625
                     /locus_tag="PPY92_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDJ2625589.1"
                     /translation="MKPYIQLVLFKQWLQYILLVTTIVIALVLIGIGYRVAHDNFKIP
                     ITIQDLDQTTASKSFVNKIKQSDYVTIKKVDEDESYIEDDVTKKEAILSMQIPKGFSQ
                     KLKENRLKETIQLYGRDDFIGGIAIEIVSSSLYEQQIPNIIYEHLEDMKQHQSIDAIN
                     KSYHKHTPESKIKFVSLTKQAQHSISISLIFAVILFVSAVQVVLHYRLNQQAALQRLS
                     QYHLSRFKLYSTYVMTHTILLLLVLLAVSLYLSQPLSLIFYLKSLLLILIYEIGIVFI
                     LFHIQTISHRLFMTFIYALAMGIVYLIIFM"
     gene            3639..4298
                     /locus_tag="PPY92_001585"
     CDS             3639..4298
                     /locus_tag="PPY92_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDJ2625590.1"
                     /translation="MIEINNLSKRYRNKQIFNHLTMSFDSNRLTVLLGDNGAGKSTLL
                     RMIAGIEKANDGTINYFGEKWNQRQIQNHIGYVPQDIALFEHMTVAENIKFFKSLCKN
                     PINDTTINEYLQQLNFDDTSAKVSTLSGGNKRKINILVGLLGQPRILILDEPTVGIDL
                     KSRHDIHQLLNIMKSKCLIILTTHHLDEVEALADDIKLIGQDPFYQHVLEDKQWAYTY
                     Y"
     gene            complement(4350..5300)
                     /gene="lytM"
                     /locus_tag="PPY92_001586"
     CDS             complement(4350..5300)
                     /gene="lytM"
                     /locus_tag="PPY92_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-glycine endopeptidase LytM"
                     /protein_id="HDJ2625591.1"
                     /translation="MKKLTAAAIATMGFATFTMAHQADAAETTNTQQAHTLMSTQSQD
                     VSYGTYYTIDSNGDYHHTPDGNWNQAMFDNKEYSYTFVDAQGHTHYFYNCYPKNANAN
                     GSGQTYVNPATAGDNNDYTASQSQQHINQYGYQSNVGPDASYYSHSNNNQAYNSHDGN
                     GKVNYPNGTSNQNGGSASKATASGHAKDASWLTSRKQLQPYGQYHGGGAHYGVDYAMP
                     ENSPVYSLTDGTVVQAGWSNYGGGNQVTIKEANSNNYQWYMHNNRLTVSAGDKVKAGD
                     QIAYSGSTGNSTAPHVHFQRMSGGIGNQYAVDPTSYLQSR"
     gene            complement(5626..6618)
                     /locus_tag="PPY92_001587"
     CDS             complement(5626..6618)
                     /locus_tag="PPY92_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDJ2625592.1"
                     /translation="MCTGFTIQTLNNQVLLGRTMDYDYPLDGSPAVTPRNYRWTSRTG
                     TTGQTQYGFIGTGTDMEGFIYGDGVNEHGVAISTQYFRGYSSYGSTHKADAMNITQNE
                     IVTWILGYTTSIEDMKQQASQIHVVAVYLNDIGEVPPLHYHVSDATGHSVEVSFKEGE
                     VVIKDNPIGVLTNHPDLDWHYSNLRQYINISPYPATAKLLEGVTIEPLGNEAGTFGLP
                     GGFTSTERFVRMAFMKANIAQNNDKEMDLMNAFYLLDAVNIPIGIVRPHDADNHYTMY
                     QTVINLTTRTLYIKYYGSNEIVALKLTDDLINRKDMTIFKPEKHITIRKLNDNQ"
     gene            6852..8228
                     /locus_tag="PPY92_001588"
     CDS             6852..8228
                     /locus_tag="PPY92_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDJ2625593.1"
                     /translation="MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTS
                     PAVLNLSISLTSFATGIFMVAAGDIADKIGQLRMTYMGLIISMFASLLLIISDITALL
                     IIGRILQGLSAAILLPSTVGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLIAT
                     NFGWQMNFIISIVIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLT
                     QGFEQGWFSTFSFICLSIFIITTLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVL
                     NSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIRLGEKATLRFGGKLMLII
                     GPLFPVIGITIISMTQLSASQYVIAVIIGFVICAIGNGLVATPGLTIAIFSMPNEKVG
                     LATGLYKMSGTLGGAFGIALSTTVFSMLQLNYAPSVAATVTFIVSIVLMILGSLSAYM
                     IIPKTVKS"
     gene            complement(8356..8748)
                     /locus_tag="PPY92_001589"
     CDS             complement(8356..8748)
                     /locus_tag="PPY92_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625594.1"
                     /translation="MQFKLKEEEIISFLELKYPEKEFEYGRLLVGQHKRDDLDVYYFG
                     DTFLMCTIISFKTFEIKETVELSYDAVNRIVLKDGWLFRKMRIETMQKVLKYGTSKLM
                     LTDFQKENYNKYIQGQKQRVIFENGHFV"
     gene            complement(8798..8977)
                     /locus_tag="PPY92_001590"
     CDS             complement(8798..8977)
                     /locus_tag="PPY92_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HDJ2625595.1"
                     /translation="MRRENILLRLIPFNDYDNKNFKMFIVMKNTKVIILKNKIFVFEQ
                     SIKKHNEMMYIELCD"
     gene            9128..10126
                     /locus_tag="PPY92_001591"
     CDS             9128..10126
                     /locus_tag="PPY92_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDJ2625596.1"
                     /translation="MKKVSIKDVAREAGVSVTTVSHILNHNDSRFSATTIKNVHAVSE
                     RLGYAPNKHAKQLRGSKIQTIGVILPSLTNPFFSALMQSIHDHKPSDVDLCFLTSTAT
                     DLYDNIKHLIDRGIDGLIIAQYISSPDALNNYLKKHHVPYVVLDQNDHQGYTDFVRTN
                     EYQGGQLAAQHLIELGHNHMIIVAPYDMMANMSTRVAGFVDTLRANQLPEPQIVHTEL
                     SKRGGLTIVDDIMVQSATAIFAINDELAIGILRGLIEHGISIPKDISLIGYDDIDYAA
                     YVSPPLTTVAQPITDIGKTSLTLLLQRLQHLDKSIDMIELPTTLKIRATTGYHLSN"
BASE COUNT         3404 a         1786 c         1606 g         3408 t
ORIGIN      
        1 aataggtgga atcgcatggg gagtttatgc attttttgca aacacaccga aaaatacata
       61 cttaaaaagc gaacaacaaa ctgcaaaaat gtataaagat tattttaatg accgttttga
      121 aaatgaagtg aagttccaag aaaagatgaa agataattcg tttttatctt cattagaatt
      181 aagcgcagat gcatcagatg aaattgttaa ggggcttggt attcctaaat ctgttgttaa
      241 tgcttcgaaa attaaaatgt catatggaca tgatcctaaa aaagagaaat caatgattaa
      301 tcttgaacca acaatcgcag actctgcatt agggaaattc cagttagctg cagataaaga
      361 taagcattat ttcgaatcac cattatttaa agggaaatat agtgttaata attctgattt
      421 attatcaacg tattcaaaac ttacaggtga agatgaagaa acagcaaaag aaaatggtat
      481 tacaaaccaa caactaaatt taaatactct ttttagtaat gctcaagcac aacaaagtga
      541 ctacagcaaa attgccgaaa aatattccga acttattgtc gacaaattag atgacgataa
      601 ttttgataaa ggtaaaaaag aagaaattaa ggttaatggt gaaaagtaca aagttagacc
      661 tgtcacgtta acacttagca gagctgacac taaaaaaatt acattagctg tattagaaga
      721 agctaaaaag gataaagacc ttaaaaaatt aatggaagaa caaggtgcta caaaagacta
      781 tgataaggac atcaaaaaag caattgacga tgtcaaagaa actaaaaagg atgaatttgc
      841 taaaattcaa tctaaaattt ataccgaaaa acatacgatt gtaaaacgag aaattactat
      901 tacagacaaa gaaaataata aaactaaaat caaaggtact aatactttag aagacgataa
      961 gttaaaacta gattacgcac ttgatttcga tcaagataaa tacacgtatg ctgaagcgaa
     1021 atatacaatt aaaggcgtat cttctaagga aaaagacaat aaatacagcg ataaatacga
     1081 atttggtaaa aagacagaat atgatgaatc aaaaatcaaa ttagataacc aagaaaaagt
     1141 agatggcaca aaacgtcaag ataaaggtaa aatcactgtc gcattagata aatatagcga
     1201 cgaaaatgaa ttcacttttg aaaataatat agattctgac gtaaaaaata acactcagaa
     1261 atctacgtta aatatcggca tcaaatatgc tgaagaacca attaatttca ttttaaaatc
     1321 tagcacaaaa ttgaaagcag atattgattt tgatgatagt ggtgcgaaag atttcaatag
     1381 tctatcttca aaagaccgtg aaaaacttga aaaagaaatc gaaaaaaatg gcggcaaaat
     1441 gtttgaatca attttaaaaa aggcatctaa ataactaaaa tataaaaata agattgaaag
     1501 gtcatcttgc ctttcaatct tatcattgga ggagtggtta aatgaaaaca atacatttgt
     1561 ttcgcatcta ccactctttt ttattgaaaa agtggtattt gattatctat ttactattta
     1621 tcttagcggc actccttatt acattaacga cgatccaaca tgtaacagaa gatgacaatc
     1681 attttaatat aggtgtcgta gataaagatc aatcaagtga aacgaaatta atcttaaact
     1741 ctattggtaa agggagtaac ctaggaaaaa atgtaagcat taaagcatat gatgataagc
     1801 aagcacatac tttgttaaaa aaacataaac ttcaaggcta ttttgttttt gataaaggta
     1861 tgaccaaggc attttataaa caaggcgaac taccaatttc agtatataca tatgatcaac
     1921 aatccatgaa aagtgtcgtg ctatctcagc taacagattc tgtttaccaa cgtcttatgc
     1981 gatcaatggg tggcatctta gcttttcaag acttagcacc gaaagcatca cattctgaca
     2041 gtatcaatgt tatgactgat ttgctgatta caggattaaa ccgttcaggt gcatttaact
     2101 tagaaccgat tcatttatac gatacgggca gttattatgc aattacagga tttttagcaa
     2161 cggtattcat ctttgcacta tctttattta cagttttgaa aatgaatcaa gatactgtat
     2221 tgaaagcgcg attgaaaatg tttcattttt ctaaagagcg tttattaatc attcgtgcgt
     2281 tgattacatg gttttatact atgttatgga gtatcgttgg tgtagtttgg attgtgttca
     2341 gtattccgaa tacctttgaa ttatataatt ggccaacgtt agccattcat ttaagctatt
     2401 atgttacctt tttaatactg tggttattac tcatcgaact tttaacaaca ggcttgttga
     2461 atagtattag taaagtcata ttagctattg tcatcctggt gttatctggt ttaacaatac
     2521 ctacgatttt tttacaacat atagcaaatg gcgttttcaa tattcaaccg ttcgcagttg
     2581 taacaaatca attgttagaa attattttaa acaactacat tttagaactg caccctagtt
     2641 tctatcttag cttcatcgca cttttgataa ttaatttagc tgtattggtt tggaggtatc
     2701 gtcaatgaag ccttatatac aacttgtttt gttcaagcaa tggttacaat acatcttgct
     2761 cgtaacaacc attgtcatcg cactcgtact tattggtatc ggttaccgtg tagcacatga
     2821 caacttcaaa ataccgatta ccattcaaga tttagaccaa accactgcat caaaatcatt
     2881 cgtcaataaa attaaacaat ctgactatgt aactattaaa aaagtcgatg aagatgaaag
     2941 ctatattgaa gatgatgtta ctaaaaagga agctatttta agcatgcaaa ttcctaaagg
     3001 tttctctcaa aaattaaaag agaaccgttt aaaagaaacg atacagttat atggtagaga
     3061 tgactttata ggtggtattg ctatagaaat tgttagtagt tcattatatg aacagcaaat
     3121 tcctaacatt atttatgaac accttgagga tatgaaacag catcaatcca tcgatgctat
     3181 caacaagtcc tatcataaac atacacctga atctaaaatc aaatttgtgt cgcttactaa
     3241 acaagcacaa cactctattt caattagctt aatctttgcg gtgattttat ttgttagcgc
     3301 tgttcaagta gtacttcatt atcgtttaaa ccaacaagca gcattgcaac gattatcaca
     3361 atatcattta agccgtttca aactatatag tacttatgta atgacacata cgatcttgtt
     3421 attgttggta ctattggcag ttagtctata tttgtctcaa ccactcagct taatatttta
     3481 cttaaaatca ttgttactta tattgattta tgagataggt atcgtcttta tcttattcca
     3541 tattcaaaca ataagtcatc gtttattcat gacatttata tatgcacttg ctatgggtat
     3601 cgtatacttg attattttca tgtaaaggag cgtaactgat gatagaaatt aataatcttt
     3661 caaagcgtta ccgtaacaaa cagattttca atcatttaac tatgtccttt gatagtaatc
     3721 gtttaaccgt attacttggt gataatggtg ctggaaaatc aacattactt cgtatgattg
     3781 ctggtattga aaaagctaat gatggaacta tcaactattt cggcgaaaaa tggaatcaaa
     3841 gacaaataca aaatcacatc ggttatgtgc cacaagacat tgcgttattt gaacacatga
     3901 cagtggctga aaacattaaa ttttttaaat cactttgtaa aaatccaatt aatgatacaa
     3961 ctatcaacga atatttacag caattaaact ttgatgatac gtctgccaaa gtatctacat
     4021 tgtccggtgg aaataaacgt aaaattaata tattagtagg tttactaggt caacctcgaa
     4081 ttctcatttt agatgaaccg acagttggta ttgatttaaa atctagacat gacatccacc
     4141 aactacttaa catcatgaaa tctaaatgtt taattatatt aactacccat catttagatg
     4201 aagtggaagc acttgcagat gatatcaagt taattggcca agatcctttt tatcaacatg
     4261 ttttagagga caagcaatgg gcttatacct attattaaaa cgaaaaaatc ccaagttgcg
     4321 tatgatattg taacttggga tttactgtat tatctacttt gcaagtatga cgttgggtct
     4381 actgcatatt gattaccgat gccaccagac atacgttgga agtgtacgtg aggcgctgtt
     4441 gaattacccg tactacctga atatgcaatt tggtcaccag ctttgacttt atcaccagct
     4501 gaaacagtta aacgattatt atgcatatac cattggtagt tattactgtt cgcttcttta
     4561 atagttactt gattgccgcc accatagtta ctccaacctg cttgtactac tgtaccatca
     4621 gttaatgagt aaactggtga attttcaggc attgcatagt cgacaccgta atgcgcacca
     4681 ccaccgtgat attgtccata tggttgtagt tgtttacgac ttgttaacca gcttgcgtct
     4741 ttcgcatgac cactagctgt cgctttactt gctgatccac cattttgatt agatgtgcca
     4801 ttaggataat tgacctttcc attaccatca tggctgttat acgcttggtt gttgttacta
     4861 tgtgaataat agctcgcgtc tggacctaca tttgattgat aaccatattg attaatatgc
     4921 tgttggcttt gactcgctgt gtagtcattg ttatctcctg ctgttgctgg attcacatat
     4981 gtttggccgc ttccattggc atttgcattt tttggataac agttataaaa ataatgcgta
     5041 tgtccttgag catctacgaa tgtatagcta tattctttat tatcaaacat tgcttgattc
     5101 cagttaccat caggtgtgtg atgataatcc ccattagaat caattgtata ataagtacca
     5161 taagatacgt cttgtgattg tgttgacatt agtgtatgtg cttgttgggt gtttgtcgtt
     5221 tctgctgcat ctgcttgatg cgccattgta aatgtagcga agcccatcgt tgcaatcgct
     5281 gctgctgtta attttttcat gtataaaaca tcctccatta aagttaaagt tagttttcaa
     5341 ttaaactgta ctgcacatac taaaagaatt agacaactga gtaaaggatt taattctcat
     5401 tttccaacta tttaatattc ccgaaatgtt ttactaaact cattacattg tcattacaaa
     5461 atagccatac attgatatta aaatgacatc tctcactgca ttcgcttaag cttttataaa
     5521 ttttcaaaat taacaactaa tcattcgtcc atgttcgcgg ttcaacgcta atgtatgata
     5581 gtaatcatcc atcaaatcat atcaaccaaa ttccattctc aatcgctatt gattgtcatt
     5641 caactttcta atagtgatat gcttctcagg cttaaaaatc gtcatatctt ttctattaat
     5701 taaatcatct gtgagcttta atgctactat ttcattgctg ccataatact taatatataa
     5761 cgttcttgta gttaaattta ttacggtctg atacatcgta tagtgattgt cagcatcatg
     5821 cggacgtaca attccaatcg gtatatttac cgcatctaat aaataaaatg cattcattaa
     5881 atccatttct ttatcattgt tttgagcaat gtttgctttc ataaatgcca ttctcacaaa
     5941 gcgctcagtt gaagtaaatc cacctggcaa tccaaatgta cctgcttcat tgcctaaagg
     6001 ttcaatcgtt acaccttcca ataactttgc tgttgctgga taaggagaaa tattgatata
     6061 ttgtcttaaa ttactataat gccaatctaa gtctggatga tttgttaaga caccaatagg
     6121 gttatctttt ataaccactt ccccctcttt aaatgaaact tcgactgaat gtccagttgc
     6181 atcggaaaca tgataatgca atggcggaac ttcaccgatg tcatttaaat ataccgctac
     6241 aacatgtatt tgggatgctt gttgtttcat atcttcaatg cttgttgtat atcccaaaat
     6301 ccatgtcaca atttcatttt gcgtaatatt catcgcgtcc gctttgtgtg ttgagccata
     6361 tgaactataa cctcggaaat attgtgtcga aatagcaacg ccatgttcat taacaccatc
     6421 accataaata aaaccttcca tatctgttcc tgtaccaata aagccatatt gcgtttggcc
     6481 tgtcgtgcca gtgcgagatg tccaacgata atttctaggc gtcactgctg gcgaaccatc
     6541 taatggataa tcataatcca tcgtgcgtcc aagaagtact tgattattta aagtttgtat
     6601 tgtgaatcct gtgcacattg ttctcactcc tctgtacctt catttacttt aatcactttc
     6661 aaataaagct gtttcactta aacatactat aaaaaatcaa tcatacaagc aattaattga
     6721 tattcattct caataactgt ggtatgatat gtaaggaaat catgacttat gtgtgagtga
     6781 acgatcatct atacatccgt tcacttcatc tcatgacttt ctatatttaa tttttacaag
     6841 gagtgacatc tgtgaataac acacaatctt caccacgcag taatattatt attgcgatta
     6901 tgttgtctgc attaacatat tggttgtttg cacaatcatt tattaatata ggacctctcg
     6961 ttggtcaaac gtatcaaacc tctcctgccg tgttaaattt atctattagt ttaacttcct
     7021 tcgccacagg tatcttcatg gtggctgcag gtgatattgc tgataaaata ggacaactga
     7081 gaatgacata catgggtctc ataatcagta tgtttgcatc tcttctatta attatatcgg
     7141 acatcactgc actgctcatc atcggtagaa ttttacaagg tctatcagca gctatcttgt
     7201 taccttcaac agttggcgtt ttaaataatc aatttaaagg agaacattta agacgagcga
     7261 ttagttatct aatgattagt actgttgggg gcatcggcct agctggtgtt atcggcggtt
     7321 taattgctac aaatttcgga tggcaaatga atttcatcat tagtatagtc attgctttca
     7381 ttgccatatt acttctaaaa ggcacacctg aaaaagtaag tcaacatagc caccgtcatc
     7441 cgttcgatta caaaggtatg tcgattttcg ctgttatgat tggtagcttt acattattgt
     7501 taacacaagg attcgaacaa ggttggttta gtacattctc attcatttgt ctgagcattt
     7561 ttatcatcac tacgctgata ttcatcatca tcgaacgtcg acatgaagta ccttttattg
     7621 atttctcagt attacgcaac cgtccgttca ttggtgcatt tttaaataac tttgttttaa
     7681 atagcggtct aggcgttaca gtggtctttt tcatatatgc tcaaacacac cttggtttat
     7741 cagctgcgca atctggactt gttacattgc catatgccat tgtggcagtt gcgatgattc
     7801 gtttaggtga aaaagcaaca ttacgtttcg gtggcaaatt gatgctcatc attggtccgt
     7861 tgttccctgt catcggcatc actattatta gcatgactca attatcggca tcacaatatg
     7921 tcattgcagt tatcattggt ttcgtcattt gcgcaattgg aaatggttta gtcgcaacac
     7981 ccggccttac aattgcaatt ttcagtatgc caaatgaaaa agttggttta gctacaggat
     8041 tatataaaat gagtggtaca ttaggtggcg cctttggtat agcactaagt actacagttt
     8101 tcagtatgtt acaactaaac tatgcaccaa gtgtagctgc aaccgtaaca tttatcgtca
     8161 gcattgtatt gatgatcctt ggctcattgt ctgcatacat gatcattcca aaaacagtta
     8221 aatcttaaat ataatagaag aattatgttt cgaaatatct ttatcacttt aaaatgatat
     8281 acaagaaatt caagaaaaat aagcgaagaa agtttttctc tgttcttcgc ttactctaat
     8341 tatattcact atcaattaga caaaatggcc attttcaaat atcacgcgtt gtttctgacc
     8401 ttgaatatat ttattataat tctctttttg aaaatcagtt aacattaatt tagatgtacc
     8461 gtattttaac actttttgca ttgtttctat tctcattttt ctaaataacc atccatcttt
     8521 taacacaata cgattaacag catcatatga taattctact gtttctttaa tttcaaatgt
     8581 cttgaatgaa ataatcgtgc acattaaaaa cgtatcacca aagtaataaa catctaaatc
     8641 atcacgttta tgttgtccaa ctaacaaacg accatattcg aactcttttt ctggatattt
     8701 caattctaaa aaactaataa tctcttcttc ttttaatttg aattgcattt aaaaacatcc
     8761 tctcttaagt tttaacaagc cttaattaaa aattttttca atcacatagt tcaatataca
     8821 tcatttcgtt atgtttttta atactttgtt caaaaacaaa tattttattc tttaaaataa
     8881 tgacttttgt atttttcatc acaataaaca ttttaaaatt cttgttatca taatcattaa
     8941 aaggtattaa ccttaataat atattctctc gtctcaacct taatcgtata cttcagacgt
     9001 ctgtttgtag acaataaaag tcattcacgt cttcatatgt catcaaatgt ttatcatgat
     9061 atgatgaata taataatcgg gtatataact gtatgattaa ttacacaata aagagaagga
     9121 atataatatg aaaaaagtgt caattaaaga tgttgctaga gaagctggtg tatcagttac
     9181 aactgtgtca catattttaa atcataatga tagtcgtttt tccgcaacaa cgataaaaaa
     9241 cgtacatgct gtttcagaac gtttaggcta tgcccctaat aaacatgcaa aacaattgcg
     9301 cggcagtaaa attcaaacta ttggcgtcat tttacctagc ttaacaaatc cgtttttctc
     9361 agcactgatg caaagtattc atgaccataa accatctgat gttgatttat gctttttaac
     9421 atctacagca actgatttgt atgacaatat taaacattta attgatcgag gtattgacgg
     9481 attaattatc gcacaataca tatcatcccc ggacgcccta aataactatc taaagaaaca
     9541 tcatgtacct tatgtcgtac tggatcaaaa tgaccatcaa ggctatacag attttgttcg
     9601 gacaaatgaa tatcaaggtg gacaacttgc agcacaacat ttaatagaac tcggtcacaa
     9661 ccatatgata attgttgcac catatgacat gatggcgaat atgtcgactc gtgtcgctgg
     9721 atttgtcgat actttgcgcg cgaatcaatt gccagaacca caaatcgtcc atactgaatt
     9781 atctaagcgc ggtgggctaa ccattgttga tgacatcatg gttcaatctg ccactgcaat
     9841 cttcgctatt aacgatgaac tcgctattgg cattttacga ggactaattg aacatggcat
     9901 cagtatcccg aaagatatct cattaatagg ttatgacgac attgattatg cagcgtacgt
     9961 ctcgccacct ttaactactg tggcacaacc tataactgat attggcaaaa catctttaac
    10021 cttattactt caacgattac agcacttaga taaatccatt gatatgattg aattaccaac
    10081 gactttaaaa attcgtgcaa caactggcta tcatctttca aactaactac gtatcttccg
    10141 aaatatactc atcattgtta ggcccttagc gttgctttaa tgctgagggt ttttaatcat
    10201 aatt
//
LOCUS       DANBWH010000094        10144 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.097, whole genome shotgun sequence.
ACCESSION   DANBWH010000094 DANBWH010000000
VERSION     DANBWH010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10144
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.097"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            143..781
                     /locus_tag="PPY92_001592"
     CDS             143..781
                     /locus_tag="PPY92_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDJ2625597.1"
                     /translation="MDDLNKKYLIDLHQHQNSSIEVLREFAEGNEVPIVDRLTLDLIK
                     QLIRMNNVKNILEIGTAIGYSSMQFASISDDIHVTTIERNETMIQYAKQNLATYHFEN
                     QVRIIEGNALEQFENVNDKVYDMIFIDAAKAQSKKFFEIYTPLLKHQGLVITDNVLYH
                     GFVSDIGIVRSRNVRQMVKKVQDYNEWLIKQPGYTTNFLNIDDGLAISIKGE"
     gene            784..1707
                     /locus_tag="PPY92_001593"
     CDS             784..1707
                     /locus_tag="PPY92_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDJ2625598.1"
                     /translation="MTELLVTPKSLSHMETLIDLGADAFVIGEQKFGLRLPGEFNRQQ
                     MTEAVALAHKNDKKVYAAVNGLFHNYHLDAVEDYINFLHEIRVDRIIFGDPAVVMYVK
                     AQENPIPLHWNAETLVTNHFQCNYWGKRGASRAVLARELNLDEIINIKENSNVEIEVQ
                     VQGMTCMFQSKRMLLGNYYTFQDRQMKIERRNDEQSLLLYDEERQNNYPVYEDYNGTH
                     IMSPNDICLIEELAPFFEAGIDSFKIDGILQTEEYINVVTEQYRQAIDLYNEDPEIYE
                     DEKFMLIDPIEEIQPDHRPFDEGFLYKQTVY"
     gene            1719..2987
                     /locus_tag="PPY92_001594"
     CDS             1719..2987
                     /locus_tag="PPY92_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDJ2625599.1"
                     /translation="MKTIEEIKSTPKTVMKKPELLAPAGNLEKLKIAVHYGADAVFLG
                     GQEYGLRSNADNFTMEEIAEGVEFANRYGAKIYVTTNIIAHDENIEGLESYLRNLEKT
                     GATGIIVADPLIIETCKEVAPKLEIHLSTQQSLSNYKAVEYWKEEGLDRVVLARETGA
                     MEMREMKEKVDIEIEAFIHGAMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYELLE
                     VDDNGELDVFYNQGEVTPFAMSPKDLKLIESIPQMMDIGVDSLKIEGRMKSIHYIATV
                     VSVYRKVIDAYAADPDNFKINPEWLIELDKCANRDTAPAFFEGTPGYEEQMFGQQQSK
                     KSPFDFCGLVLDYNEDTKIATIQQRNNFKPGQEIEFFGPEIETFTQVVEAIYDEEGNS
                     LDAARHPLQIVQIKVDRPIYPNNMMRKEIG"
     gene            2987..3610
                     /gene="udk"
                     /locus_tag="PPY92_001595"
     CDS             2987..3610
                     /gene="udk"
                     /locus_tag="PPY92_001595"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDJ2625600.1"
                     /translation="MKATTIIGIAGGSGSGKTTVTNEIMKNLEGHSVALLAQDYYYKD
                     QKHLTFDERLETNYDHPFAFDNDLLIENLKDLKNGKAVEVPTYDYASHTRSDITIDFK
                     PKDVIIVEGIFALENKVLRDMMDVKIYVDTDADLRILRRLTRDTKERGRSMDSVINQY
                     LSVVRPMHDQFIEPTKKYADIIIPEGGSNKVAIDIMTTKIQSLVSKQ"
     gene            3638..4114
                     /gene="greA"
                     /locus_tag="PPY92_001596"
     CDS             3638..4114
                     /gene="greA"
                     /locus_tag="PPY92_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDJ2625601.1"
                     /translation="MENQKQYPMTQEGFEKLERELEELKTVKRPEVVEKIKVARSFGD
                     LSENSEYDAAKDEQGFIEQDIQRIEHMLRNALIIEDTGDNNVVKIGKTVTFVELPGDE
                     EESYQIVGSAESDAFNGKISNESPMAKALIGKGLDDEVRVPLPNGGEMNVKIVNIQ"
     gene            4440..5174
                     /gene="pxpB"
                     /locus_tag="PPY92_001597"
     CDS             4440..5174
                     /gene="pxpB"
                     /locus_tag="PPY92_001597"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDJ2625602.1"
                     /translation="MDVRFINEQTIMIYFENKISEETYRNVTAMVRWIREKEILEIQD
                     IVPSYRAVLIYFDEQAITSSKLIENLELNKFNEKNVHAVNQTNRIIKIPVQYGGTYGP
                     DIEEVAKHNRITVEQVIEKHTSKPYLIYMLGFMPGFPYLGGLDEQLHTPRRNQPRLKI
                     HAGSVGIANNQTGLYPSDSPGGWQIIGRTPLKVFSSEREPMSMYEAGEWIQFYAIDEQ
                     KFIQIERDISDGNFNVDDWVVIENVN"
     gene            5164..6174
                     /locus_tag="PPY92_001598"
     CDS             5164..6174
                     /locus_tag="PPY92_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HDJ2625603.1"
                     /translation="MSIKILQPGLFSTVQDLGRIGYEHIGFSGAGAMDQFSFKVAQSL
                     INNDGPAIEYTLIGPTIQFNTQNTFVITGGSINASLNNKTISMNSVILAEKGDILKIG
                     AITKGVRGYLTFGHSINVPSIAESYATHTRSSIGGFKGRKLLANDVITVKINNDFKEN
                     IGKTIHLQDDLLPENNIIHILQGPQFEAFSEEARAKIVNHPYLITEQSDRMGYRLEGD
                     SVAPINQADIISEPVALGSVQVPNDGQPIILLNDKQTIGGYTKIATVCKFDLPKLAQM
                     KPKDTIQFKWISFQEAVDKNREQMSLFNEILKSHQKTPIFDTSSLRHTSKKLSTILKG
                     DL"
     gene            6176..6625
                     /locus_tag="PPY92_001599"
     CDS             6176..6625
                     /locus_tag="PPY92_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HDJ2625604.1"
                     /translation="MNIEKIEQIIKLVKENDVKKFKYKNFEDEIEIDFTDSNHLAAHS
                     NQSNQSMNNNDLTASKANDNSDVSTNDYHDIKSPMVGTFFLQDSKELTEPIVNVGDKV
                     NKGDIIGYVEAMKVLNEVTTDVAGEITEIVADHGTNVEYDQVLVRIK"
     gene            6639..8000
                     /locus_tag="PPY92_001600"
     CDS             6639..8000
                     /locus_tag="PPY92_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDJ2625605.1"
                     /translation="MLRCLIANRGEIAVRIIRACREYGIETVAVYAKGDEQSLHVHLA
                     DQAICIGEANALDSYLNIDRIISAAQITGANAIHPGYGFLSESTKFAQTVEEQGIAFI
                     GPTKKTMEMMGDKITARQTVHHAGVPVIPGSNGAVNHVSEIENLAKDIGYPVVIKAAS
                     GGGGKGIRIVKKAEDLEKAFKEAKSEGKKYFDDDRVYVEAFIPVAKHVEVQVMGDGQD
                     NYVHLGERDCSVQRKNQKLIEESPCAALTEERRQQICNDAVKVARAANYRSAGTIEFL
                     VTDTAHYFIEMNARIQVEHTVTEMRAERDLVAAQLYLLEHNHLPFSQSDIQFNGHVIE
                     ARINAENPEKKFQPTPGKVTALHLPQGFNVRVDSLLYHGYQVSPYYDSLVAKVIVKSH
                     DRASAIDKLKVTLDEMVIDGFSTTADFLYAVLNYPLYRDGDAKDVDIKFLEKHQIVKG
                     VEL"
     gene            8000..8752
                     /gene="pxpA"
                     /locus_tag="PPY92_001601"
     CDS             8000..8752
                     /gene="pxpA"
                     /locus_tag="PPY92_001601"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDJ2625606.1"
                     /translation="MRVDLNCDLGEAFGNYSFGGDHQIIPLITSANVACGFHAGDENV
                     MNETVKLAKAHNVAVGAHPGLPDLKGFGRRNIDISNDEIYNLMIYQLGALQGFCRIHQ
                     LKINHVKPHGALYQMGAKDREIANVIAQAVYDFDPSLVLVGLANSYLISEAKNVGLIT
                     ASEVFADRRYEDDGQLVSRKESDAVITDTDEALKQVLKMVKENKVISKNNKEVTLQAD
                     TICVHGDGEHALLFVSKIREILMKEGIDIQSL"
     gene            8765..9994
                     /locus_tag="PPY92_001602"
     CDS             8765..9994
                     /locus_tag="PPY92_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDJ2625607.1"
                     /translation="MGRNLKLKKESDFEFTKNHKRLLLGSVFLMATSAIGPAFLTQTA
                     VFTSQFFASFAFAILLSIIIDIGAQINIWRILVVTGLRGQEISNKVVPGLGTVISILI
                     AFGGLAFNIGNIAGAGLGLNAIFGLDVKWGAAITAIFAILIFVSKSGQKIMDVVSMIL
                     GIVMILVVAYVMFVSNPPYGDAFVHTFAPEHPMKLVLPIITLVGGTVGGYITFAGAHR
                     ILDSGIKGKQYLPFVNQSAIAGILTTGIMRTLLFLAVLGVVVTGVTLSSENPPASVFE
                     HAIGPIGKNIFGIVLFAAAMSSVIGSAYTSATFLKTLHKSLNERSNLIVIVFIVISTM
                     IFLFIGKPISLLIIAGAINGWILPITLGAILIASKKKSIVGDYKHPNWMFIFGIVAVL
                     VTILTGIFSFKEVLQLF"
BASE COUNT         3626 a         1401 c         2000 g         3117 t
ORIGIN      
        1 cgcttctata aataaaatag ctttgcgtct aacaagaaat cagtcattta attggttggc
       61 aaaattataa tcaattgtga aggctgattt attttattgt cataatcata ttgacttatt
      121 ttacataaag gaagtttaaa acatggatga cctaaataaa aaatatttaa tagatttaca
      181 tcaacatcaa aatagttcaa tcgaagtttt gcgtgaattt gccgagggaa atgaagtgcc
      241 aattgtagat cgtttaacat tagatttaat taagcaatta attcgtatga ataatgttaa
      301 aaatatttta gaaattggta cagcaatcgg ctatagttct atgcaattcg cttctatatc
      361 tgatgacatt catgtcacaa cgatagagcg taatgaaacg atgattcaat atgctaaaca
      421 aaatttagct acttatcatt ttgaaaacca ggttcgaatt attgaaggta atgctttaga
      481 gcaatttgaa aatgtaaatg acaaagttta tgatatgata tttattgatg cagcaaaagc
      541 gcaatcaaag aaattttttg aaatatatac accactttta aagcaccaag gtctcgtaat
      601 tacagataat gttttatatc acggttttgt atcggatatt gggattgtta gatcgagaaa
      661 tgtaagacaa atggttaaaa aggttcaaga ttataatgag tggttaataa agcaaccagg
      721 atatacaacg aattttttaa atatagacga tggattagcg atttcaatta aaggagaatg
      781 actatgacag aattacttgt cacacccaag tcattaagcc atatggaaac acttattgat
      841 ttaggtgcag atgcttttgt aattggcgag caaaagtttg gtttaagact tccaggggaa
      901 ttcaatagac aacaaatgac agaggctgtc gcattagctc acaaaaacga taaaaaagta
      961 tatgcggcag tgaatggatt atttcataat tatcatttag atgctgtaga agattatatt
     1021 aattttttac atgaaatacg agttgacaga attattttcg gggatccagc agtagtaatg
     1081 tatgtgaagg cgcaagaaaa tccaatacca cttcattgga atgcagagac gttagtaact
     1141 aatcattttc aatgtaatta ctggggtaaa agaggcgcat caagagctgt cttagcacgt
     1201 gagctgaatt tggatgaaat tattaatata aaagaaaatt caaatgtgga aattgaggtg
     1261 caagttcaag gaatgacttg tatgttccaa tcaaagcgta tgctactagg caattattat
     1321 acgttccaag atcgccaaat gaaaattgaa cgtcgcaatg atgaacaatc tttattactt
     1381 tatgatgaag aaagacaaaa taattaccca gtttacgaag attacaatgg tacgcacatt
     1441 atgtcaccaa acgacatttg tttaattgaa gaattagcac cattttttga ggcgggtata
     1501 gattcattta aaatcgatgg tattctacaa acggaagaat atattaatgt ggtaacggaa
     1561 cagtatcgtc aagcgataga tttgtacaat gaagatcctg aaatctatga agatgagaaa
     1621 tttatgttga ttgatccaat tgaagaaatt caacctgatc atcgaccatt tgacgaaggt
     1681 ttcttatata aacaaacagt atattaagga ggttaatcat gaagacaata gaagagatta
     1741 aatcaactcc taaaacagtt atgaagaaac cagaattatt agcacctgct ggaaacttag
     1801 aaaagttaaa aatagcagta cattatggcg ctgatgccgt atttttaggt ggtcaagaat
     1861 atggattacg ttcaaatgct gataatttca cgatggaaga aatagctgaa ggtgttgaat
     1921 ttgcgaaccg ttacggtgcc aaaatttatg ttacgacaaa tattattgca catgatgaga
     1981 atattgaagg tctagaatca tatttgcgta atttggaaaa gactggtgcg acaggtatca
     2041 ttgttgcaga tcctttaatt atcgaaacat gtaaagaagt tgcgccaaaa cttgaaattc
     2101 atttatctac tcaacaatca ctttctaatt acaaagctgt agaatattgg aaagaagaag
     2161 gattggatcg tgttgtatta gcacgtgaga ccggcgcgat ggaaatgcgt gaaatgaagg
     2221 aaaaagtaga tattgaaatc gaagcattta ttcatggtgc tatgtgtatc gcctattcag
     2281 gtagatgtac attaagtaat catatgactg caagggattc caacagaggc ggttgctgtc
     2341 aaagttgccg ttgggattat gaattattag aagttgatga taatggtgaa cttgatgtat
     2401 tttataatca aggtgaagtt acaccgtttg cgatgagtcc taaagattta aaattaatcg
     2461 aatcaattcc tcaaatgatg gatattggtg tggactcatt aaaaattgaa ggacgtatga
     2521 agtcaattca ttatattgca acagttgtct cagtatatcg taaagtcatt gatgcgtatg
     2581 cggcagatcc tgacaacttt aagattaatc cggaatggtt aattgagtta gataaatgtg
     2641 caaatagaga cactgcacca gcattctttg agggaacacc tggttatgaa gaacagatgt
     2701 ttggtcaaca acaatctaaa aaatcaccat ttgatttttg tggtttggta ttagactata
     2761 atgaagatac aaaaattgcg actattcaac aacgaaataa ctttaaacca ggtcaagaaa
     2821 tagaattctt tggtccagaa attgaaacat ttacacaagt agtagaagca atttatgatg
     2881 aagaaggtaa cagcttagac gcggcgcgcc atccattaca aatcgttcaa attaaagtgg
     2941 atcgcccgat atatccaaac aacatgatga gaaaggaaat tggctaatga aagctactac
     3001 aatcattggc atagctggtg gatctggctc aggaaaaaca actgtaacta acgaaattat
     3061 gaaaaactta gaaggtcata gtgtcgcttt acttgctcaa gattactatt ataaagatca
     3121 aaagcacttg actttcgacg agcgcctaga aaccaattat gaccatccat ttgcattcga
     3181 taatgattta ttaattgaaa atcttaaaga cttgaaaaat ggtaaagcag tagaagtacc
     3241 gacatatgat tatgctagtc atacaagaag tgacattacc attgatttta aacctaaaga
     3301 tgttattatc gtagaaggta ttttcgcttt agaaaataag gtattacgtg atatgatgga
     3361 tgttaaaata tatgttgata cagatgcaga cttgagaata ttacgccgtt taacacgaga
     3421 tactaaagag cgtgggcgtt caatggactc tgttatcaat caatatttaa gtgttgttag
     3481 acctatgcat gaccaattta ttgaaccgac taagaaatat gctgatataa ttattcctga
     3541 aggtgggagc aataaagttg caatagatat tatgacaaca aaaattcagt ctttagttag
     3601 caagcaatag tataattcag taaaggaaga tgacaatatg gaaaatcaaa agcaatatcc
     3661 aatgactcaa gaaggttttg aaaaattaga gcgtgaactt gaagaattaa aaacagttaa
     3721 gcgtcctgaa gttgtagaga aaattaaagt tgcacgttca tttggtgact tatcagagaa
     3781 ctctgagtat gatgcagcaa aagatgaaca aggattcatc gaacaagata ttcaaagaat
     3841 tgagcatatg ttaagaaatg cattaatcat tgaagatact ggagataaca acgttgttaa
     3901 aattggtaaa acagtaacgt ttgtagaatt accaggtgat gaagaggaaa gttatcaaat
     3961 cgttggttca gctgaatcag atgcatttaa tggtaagatt tcaaatgaat caccaatggc
     4021 taaagcgtta attggtaaag gtttagatga tgaagttcgt gttccactac ctaatggtgg
     4081 cgaaatgaac gtaaaaattg ttaatatcca ataaatgatc aaatagattg tttaattgcg
     4141 cgattgaagt gtgttaatga tacttcggtc gtgctttttt atattcaaaa tcaaaaaatc
     4201 ggacagatga aaagtaaaaa ttttccatca gaccgaatga tagcagaacc taaattagtg
     4261 taagagttac aatattaact actccatttt aaaaaagcaa aatatttccg agaaaacaaa
     4321 ttgattatca tttgttattg tatgcgattt gaattactta ttaaacaact tgtgcttctc
     4381 aatgatacaa tgaaaaggta ttcaatactg gtttattgca atacaaaggg ggatttaaag
     4441 tggatgtaag atttattaac gagcagacaa ttatgattta ttttgaaaat aaaatatccg
     4501 aagagacata tcgtaatgtc acagcaatgg tacggtggat acgagaaaaa gaaatattag
     4561 aaattcaaga tattgtccct tcgtatcgag cagttctaat atattttgat gaacaagcta
     4621 taacatcttc taaattaatt gaaaatttag aacttaacaa atttaatgaa aaaaatgttc
     4681 atgctgtgaa tcaaaccaat cgtattatta aaatacctgt tcaatatggt ggtacatatg
     4741 gaccagatat tgaagaagta gcaaagcata atcgaataac tgttgagcaa gttattgaaa
     4801 aacatacaag taaaccttat ttaatatata tgctaggatt tatgccagga tttccatact
     4861 taggcggact tgatgaacaa ctacatacac ctagacgtaa tcagccaagg ttaaaaattc
     4921 atgcaggttc tgtaggtatt gcgaataatc aaacaggact atatccatca gattctcctg
     4981 gcggatggca aattattgga cgcacaccgt taaaagtatt tagcagcgag cgtgaaccaa
     5041 tgtcaatgta cgaagcagga gaatggatac aattttatgc aatagatgaa cagaaattta
     5101 tacaaatcga aagagatata tcagatggca actttaatgt tgatgactgg gtggtgattg
     5161 aaaatgtcaa ttaagatttt acaaccaggt ctcttttcaa cggtacaaga tctaggaaga
     5221 ataggttatg aacatattgg attttcaggc gcaggtgcca tggatcaatt tagttttaag
     5281 gttgcgcagt cattaattaa caatgatggt ccagcgattg aatatacttt gattggtcct
     5341 accattcaat ttaatacgca aaatacattt gttataaccg gtggtagtat taatgcttcg
     5401 ctaaataata aaactatatc aatgaattct gtcatattag ctgagaaagg tgacatttta
     5461 aaaataggtg ctataaccaa aggtgtacgc ggttatctta cttttggtca ttctatcaac
     5521 gtaccttcta ttgctgaaag ttatgcaaca catacccgaa gcagtatagg aggattcaaa
     5581 ggtagaaagt tattagctaa tgatgtaata acagtgaaaa taaataatga ctttaaagaa
     5641 aacattggaa agactattca tttacaggac gatttattgc cagaaaataa tattatacac
     5701 attcttcaag gacctcaatt cgaggcattt tctgaagagg ctagagcgaa aattgtaaat
     5761 catccatatt taattactga acaatcagac cgtatgggct atcgtttgga aggtgacagc
     5821 gttgcaccaa ttaatcaagc agatatcatt tctgaaccgg ttgctcttgg tagtgttcaa
     5881 gtaccaaatg atggtcaacc tattattctg cttaatgata aacaaacgat aggtggttat
     5941 accaaaattg caactgtatg caaatttgat ctgccaaagt tagcacagat gaaaccaaaa
     6001 gatacaattc aatttaaatg gatatcattc caagaagctg tagataagaa tcgggaacaa
     6061 atgtctctgt ttaatgaaat tcttaagagt catcaaaaga ctccaatttt tgatacatct
     6121 agcttgcgac atacttcgaa aaaattatca acaattttaa aaggggattt gtaaaatgaa
     6181 tattgaaaaa atcgaacaaa taatcaaatt agtgaaggaa aatgatgtta agaaatttaa
     6241 atataaaaat tttgaagatg aaatagaaat tgacttcact gactcgaatc atttggctgc
     6301 acacagtaat caatcaaatc aaagtatgaa caataatgat ttgacagctt caaaagcgaa
     6361 tgataactcc gatgtttcga caaatgatta tcatgacatt aaatcaccaa tggtaggtac
     6421 attcttttta caagatagta aagaattaac tgaaccaatt gtgaatgtcg gtgacaaagt
     6481 taacaaggga gatattatag gatatgttga agcgatgaaa gtattaaacg aggtaacaac
     6541 agatgttgct ggagaaatta ctgaaatagt agctgatcat ggcacaaatg ttgaatacga
     6601 ccaagttttg gtacgtatta agtaaagggg gtaagtaaat gcttcgttgt ttaattgcga
     6661 acagaggtga aattgctgta aggattataa gagcttgcag agaatatggg attgaaactg
     6721 tagcagttta tgcaaaaggg gacgagcaaa gcttgcatgt acatttagca gatcaagcta
     6781 tatgtattgg ggaagctaat gctttagata gttatttaaa tattgaccgc atcatatctg
     6841 ctgcacaaat cactggtgct aatgcaattc acccaggata tggcttttta tcagaatcaa
     6901 caaaatttgc tcaaaccgtt gaggaacaag gcatagcgtt tatagggcct actaaaaaaa
     6961 cgatggaaat gatgggggat aaaataacag caagacaaac agttcatcat gcaggtgttc
     7021 ctgtgattcc aggatcaaat ggtgctgtga atcatgtgtc tgaaattgag aatcttgcca
     7081 aagacatcgg ttatccagtt gttatcaaag ctgccagtgg cggtggtggt aaaggtatac
     7141 gtattgtaaa gaaagctgaa gatttagaaa aggcatttaa agaagctaaa agtgaaggaa
     7201 aaaaatactt tgatgatgat cgtgtttatg ttgaggcttt tatacctgtt gcaaaacatg
     7261 ttgaagttca agttatggga gatgggcagg ataattatgt acatttaggg gaacgtgatt
     7321 gttctgtaca acgtaaaaat caaaaattaa tcgaagaatc tccttgtgca gcattaactg
     7381 aagaacgacg acaacaaata tgtaacgatg ctgttaaagt tgcacgtgca gcaaattata
     7441 gaagtgcagg tacgattgaa tttttagtaa ctgataccgc acattacttt attgaaatga
     7501 atgctcgaat acaagttgaa catactgtta cagagatgcg agcagaacgt gatttggttg
     7561 cagcacaact gtatttattg gaacataatc atttaccttt ttcacaaagc gatattcaat
     7621 ttaatggtca tgtgatagaa gcgagaataa atgccgaaaa tcctgaaaag aaatttcaac
     7681 caacaccagg taaagtgaca gcgttacatt tacctcaagg ctttaatgtg cgagtcgatt
     7741 ctttactata tcacggttat caagtttcac catattatga ttctttagta gctaaagtaa
     7801 ttgtaaaatc tcatgataga gcttcagcaa ttgataagtt gaaagtcacc ttagatgaaa
     7861 tggtgataga tggattttct acaactgccg attttttata tgcagtttta aattatccac
     7921 tatatcgtga tggagatgca aaagatgttg atattaaatt tttagaaaaa catcaaattg
     7981 ttaaaggggt ggaattataa tgcgagttga tttgaattgt gatttaggcg aagcatttgg
     8041 aaattattcc tttggtggtg atcatcaaat tattccgtta attacaagtg cgaatgttgc
     8101 ttgtggtttt cacgctggtg atgaaaatgt aatgaatgaa acggtaaaac ttgccaaagc
     8161 acataatgtt gcagtaggtg cacatcctgg tttacctgat ttgaaaggct ttggcagacg
     8221 aaatatagat atctctaacg acgagattta taatttgatg atttatcaat taggtgcatt
     8281 acaagggttt tgtcgcattc atcaacttaa aattaatcat gttaaaccgc atggtgcatt
     8341 gtatcagatg ggtgcaaaag acagagaaat agcaaacgtt atagcacaag ctgtttatga
     8401 ctttgatcca tcactagtgt tagtaggatt agcaaattca tatctaattt cagaagcaaa
     8461 gaatgtcgga ttaattacag cttctgaagt gtttgctgat agacgatacg aagatgatgg
     8521 gcagctcgtt agtagaaaag aaagtgatgc tgtgattact gatactgacg aagcacttaa
     8581 gcaggtttta aagatggtga aggaaaataa agttatttca aaaaacaata aggaagtaac
     8641 gttacaagca gatacaattt gtgtgcatgg tgatggagaa catgcattat tatttgtttc
     8701 gaaaattaga gaaattttaa tgaaagaagg cattgatatt caatccttat agggggctaa
     8761 aaaaatgggg agaaatttaa agcttaaaaa agaaagtgac tttgaattca caaaaaatca
     8821 taaaaggtta ttattaggtt ctgtattttt gatggcaact tctgcaattg ggccagcatt
     8881 tttaacgcaa acagcagtat ttacatcaca attttttgca agtttcgcat ttgccatatt
     8941 actgtctatc atcattgaca ttggtgcaca aattaatata tggcgcatat tagttgtaac
     9001 tggtttaaga ggtcaagaaa tatcaaataa agttgttcct gggcttggta ctgttatctc
     9061 aatattgatt gcatttggtg gtctagcttt taatattggt aatattgctg gtgccggttt
     9121 aggtttaaat gcaatttttg gattagatgt aaaatggggc gcagctatta ctgcaatctt
     9181 tgcaatatta atctttgtaa gtaaaagtgg ccaaaaaatt atggacgttg tttcaatgat
     9241 tcttggtatt gtgatgattt tagttgtggc atatgtgatg tttgtttcta atccacctta
     9301 tggtgatgct tttgtgcata catttgcgcc agaacatcca atgaaattag tcttgcccat
     9361 cattacgtta gttggtggaa ctgtaggtgg ttatattacc tttgcaggtg cacatcgtat
     9421 attagactct ggcattaaag gtaagcaata tttaccattt gtaaatcaat cagcaattgc
     9481 tggtatttta actacaggta ttatgagaac gttactattc ctagcggtat taggagttgt
     9541 tgtaacaggt gtgacactaa gttctgaaaa tccaccagcg tcagtttttg aacacgcaat
     9601 tggaccaatt ggaaagaata tttttggtat tgtgttattt gctgcagcta tgtcatcagt
     9661 aattggctca gcatacacaa gcgcaacatt tttaaaaaca cttcataaat cacttaacga
     9721 aagaagtaat ttaattgtga ttgtgtttat cgttatttca acaatgattt tcttatttat
     9781 tggaaaacca atcagccttt taattatagc aggcgcgata aatggatgga ttttacctat
     9841 tacgttaggt gcaattttga ttgcaagtaa aaagaaatca attgttggtg attataaaca
     9901 cccaaattgg atgttcatct ttggtatcgt tgcagtactt gttacaatat tgacaggtat
     9961 attctcattc aaagaagtac ttcagttatt ttaaaagaca tttcgactaa tagcatcact
    10021 gtaaatgttg ttagtattag gcaagatata ttttgatttc cggtgattca tttataaagc
    10081 ataattagat atattttaag ggagagtggt gcatgacaaa tgtactgctc tttttgttgt
    10141 gatg
//
LOCUS       DANBWH010000095        10031 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.098, whole genome shotgun sequence.
ACCESSION   DANBWH010000095 DANBWH010000000
VERSION     DANBWH010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10031
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.098"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            117..1325
                     /gene="gltS"
                     /locus_tag="PPY92_001603"
     CDS             117..1325
                     /gene="gltS"
                     /locus_tag="PPY92_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HDJ2625608.1"
                     /translation="MIELNAITTLCLACILYLLGTAIVNHVNFLKRICIPAPVIGGLI
                     FAILVAALDSFGMVKIKLDASFIQDFFMLAFFTTIGLGASLKLFKLGGKVLLLYFMFC
                     AIISVIQNIVGVSLAKVLNIKPLLGLTAGSMSMEGGHGNAAAYGKTIQDLGIDSALTA
                     ALAAATLGLVFGGLIGGPVVKYLIKRYNLKPQHSDDTFKDYSQVAYNEHLHSKFNATE
                     VFFIQFTIVVFCMAVGSYFSHLFTAQTGINVPIYVGSLFVAVIVRNISESFNFNIVDL
                     KITNQIGDVALGIFLSLALMSIQLIEIYKLAIPLIIIVLVQVVVMILFAVLILFRGLG
                     KDYDAAVMVGGFIGHGLGATPNAMANLDVITKKYGNSPKAYLVVPIVGAFLIDLIGVI
                     VIMGFIQWFS"
     gene            complement(1373..2422)
                     /locus_tag="PPY92_001604"
     CDS             complement(1373..2422)
                     /locus_tag="PPY92_001604"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDJ2625609.1"
                     /translation="MSDFQREQRKNEHVEIAMAQSDAMHSDFDKMRFVHHSIPSINVN
                     DIDLTSQTPDLTMAYPIYINAMTGGSEWTKNINEKLAVVARETRLAMAVGSTHAALRN
                     PRMAETFTIARKMNPEGMIFSNVGADVPVEKALEAVELLEAQALQIHVNSPQELVMPE
                     GNREFVTWLDNIASIVSRVSVPVIIKEVGFGMSKELMHDLQQIGVKYVDVSGKGGTNF
                     VDIENERRANKDMDYLSSWGQSTVESLLETTAYQSEISVFASGGLRTPLDAIKSLALG
                     AKATGMSRPFLNQVENNGIAHTVAYVESFIEHMKSIMTMLDAKNIDDLTQKQIVFSPE
                     IMSWIEQRSLNIHRG"
     gene            complement(2453..3400)
                     /gene="corA"
                     /locus_tag="PPY92_001605"
     CDS             complement(2453..3400)
                     /gene="corA"
                     /locus_tag="PPY92_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDJ2625610.1"
                     /translation="MALKIRYQTTYEPFKVVDDIKEIPKDATIVWYDFDEPNEQENEW
                     FKAHFNFNDLEVDDAINGMPRAKYKSYKDYQYLVFHSIMGSNYSPIALNIFIQDNVLV
                     TYHHQTLESLNKVVYKYMNTLDAELDCADVVILILDMMVDKYFNFVYALEDSVYHFED
                     RHVDDRFNKMVMDSVFKLRSDLIKVKRVLFPMQELIDTMKQNGDLIIDNKHSLYIQHI
                     DDHLIKQRNIIRTAQEMTNEIRENFESYTSFRMNSIMQVLTLVSVIFSPLTFIAGIYG
                     MNFVNMPALHLHYGYYICLAVMFVIAVVLIIFFRRKKWF"
     gene            3497..3988
                     /locus_tag="PPY92_001606"
     CDS             3497..3988
                     /locus_tag="PPY92_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HDJ2625611.1"
                     /translation="MVIIKQLFTHTQTVTSEFIDHNNHMHDANYNIIFSDVVNRFNYS
                     HGLSLKERENLAYTLFTLEEHTTYLSELSLGDVFTVTLYIYDYDYKRLHLFLTLTKED
                     GTLASTNEVMMMGINQHTRRSDAFPESFSTQIAHYYKNQPTITWPEQLGHKIAIPHKG
                     ALK"
     gene            3985..4404
                     /locus_tag="PPY92_001607"
     CDS             3985..4404
                     /locus_tag="PPY92_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625612.1"
                     /translation="MTDALQQKIHIELLDLLDDVKFELTELNAQKGLYINGPANQLLK
                     RGVHMAYVQGQKQAIDNIMTIVEQQLEDQHFLEDYDKFQNEVTHRNYDKTANFAELSD
                     IPRQFDNFLDQFYQIKGQYFIITHINTLIGDFHSEAH"
     gene            complement(4512..5432)
                     /locus_tag="PPY92_001608"
     CDS             complement(4512..5432)
                     /locus_tag="PPY92_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDJ2625613.1"
                     /translation="MIRNRVMNSVVNKYLLHNRSIMFKNDQDVERFFYKREIENRKKH
                     KQPSTLNVKANLEKLSLDDMQVFRFNFRHQIDKKILYIHGGFNALQPSPFHWRLLDKI
                     TLSTLYEVVLPIYPKTPEFHIDDTFQAIQRVYDQLVSEVGHQNVVVMGDGSGGALALS
                     FVQSLLDNQQPLPNKLYLISPILDATLSNKDISDALIEQDAVLSQFGVNEIMKKWANG
                     LPLTDKRISPINGTIEGLPPVYMFGGGREMTHPDMKLFEQMMLQHHQYIEFYDYPKMV
                     HDFPIYPIRQSHKAIKQIAKSIDEDVTQNN"
     gene            5640..6278
                     /locus_tag="PPY92_001609"
     CDS             5640..6278
                     /locus_tag="PPY92_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625614.1"
                     /translation="MTNPNQRLEPFDETFQQPNIHRGKRYGKKKRSLVSMIIQIIVVI
                     LTAIAGYSMWKQPIFNIVFTKESVDFDGIKNFNETIGKMHQLNINLGNINDLQQGIDR
                     LILVFNGFFILCIVSLVITILTIVFNRTVLKVVNILVLTIMLVITLYFSYIIKTLAQE
                     IADALKKYYLNVPPDQVITEADAIHNGIILLGCSIGLLVISLFFRNRMPRLK"
     gene            complement(6524..8455)
                     /locus_tag="PPY92_001610"
     CDS             complement(6524..8455)
                     /locus_tag="PPY92_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HDJ2625615.1"
                     /translation="MTTTFIISYIILALIIVGVINLFLIRSRKKGKRQQKEQQFTTRQ
                     SNQSKFKASDLDKTTDQSTQRMTHEELRVDNQDDHSQVSLNGYTKGSEKDQEAFTNNN
                     GEEAVAAKNPESEEYKVNEKIKKEHKNFIFGEGVSRGKILAALLFGMFIAILNQTLLN
                     VALPKINTEFNISASTGQWLMTGFMLVNGILIPITAYLFNKYSYRKLFLVALVLFTIG
                     SLICAISMNFPIMMVGRVLQAIGAGVLMPLGSIVIITIYPPEKRGAAMGTMGIAMILA
                     PAIGPTLSGYIVQNYHWNVMFYGMFIIGIIAILVGFVWFKLYQYTTNPKADIPGIIFS
                     TIGFGALLYGFSEAGNKGWGSVEIETMFAIGIIFIILFVIRELRMKSPMLNLEVLKFP
                     TFTLTTIINMVVMLSLYGGMILLPIYLQNLRGFSALDSGLLLLPGSLIMGLLGPFAGK
                     LLDTIGLKPLAIFGIAVMTYATWELTKLNMDTPYMTIMGIYVLRSFGMAFIMMPMVTA
                     AINALPGRLASHGNAFLNTMRQLAGSIGTAILVTVMTTQTTQHLSAFGEELDKTNPVV
                     QDHMRELASQYGGQEGAMKVLLQFVNKLATVEGINDAFIVATIFSIIALILCLFLQSN
                     KKAKATAQKLEASSNNNHE"
     gene            complement(8468..9115)
                     /locus_tag="PPY92_001611"
     CDS             complement(8468..9115)
                     /locus_tag="PPY92_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDJ2625616.1"
                     /translation="MKKMILINVITVVVLLAIGIAGFYFWNKTTSYVTTDNAKVNGDQ
                     IKIASPASGQIKSLNVKQGDKLDKGDKVATVTVQGQDGETKDMDLKMPQKGTIAKLDG
                     MEGSMVQAGNPIAYAYNLDDLYVTANIDEKDIKDVEVGKDVDVTIDGQKASIKGKVDS
                     IGKATAASFSLMPSSNSDGNYTKVSQVIPVKITLESEPSKQVVPGMNAEVKIHKN"
     gene            9236..9790
                     /locus_tag="PPY92_001612"
     CDS             9236..9790
                     /locus_tag="PPY92_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDJ2625617.1"
                     /translation="MKRQAKIEIQNALVDLMAEYPFQEISTKMICAYCNINRSTFYDY
                     YKDKFDLLDTINSKHKEKFQFLLSALHHNFENIKQDKLKLYKFFIIIAKYIKHNEQFF
                     KDILVTYPMKTLFIDYINLARDYYQQIMNDYSTTVTNKQLYVTYIIGGQAGVFINWLS
                     NGCNESPEEVADILLANTIKLQIQ"
BASE COUNT         3284 a         1743 c         1476 g         3528 t
ORIGIN      
        1 ttcaaaaact agaaatattg atttgttgca tgtataatgt taaaagtgcc cttttataac
       61 gcttacatat aaaagcttat ttagggagag ggatattcaa caagggggat ttgaaaatga
      121 tagaacttaa tgcaattaca acattatgtt tagcctgtat actttactta cttggtacag
      181 ctatcgttaa tcacgttaat tttttgaaac gcatttgtat accagcacca gtgattggcg
      241 gcttaatctt tgctatttta gttgcggctt tggattcatt tggcatggtt aagattaaat
      301 tagatgcttc attcattcaa gatttcttca tgttagcatt ctttacgaca atcggtcttg
      361 gtgcatcatt gaaattattt aaattaggtg gcaaagtctt gctattatac tttatgtttt
      421 gtgctatcat ttcagtcatt caaaacatag ttggtgtatc actagcaaaa gtattaaata
      481 ttaaaccgtt actaggatta acagcaggtt ccatgtctat ggaaggcggt catggtaatg
      541 ctgctgctta tggtaagaca attcaagatt taggtattga ttcggcactg acagcggctc
      601 ttgcagctgc aactttaggt cttgtatttg gagggcttat cggtggtcca gttgtaaaat
      661 atttaatcaa acgttataac ttaaagcctc aacatagtga cgatacattt aaagattata
      721 gccaagtagc atataacgaa catttacata gtaaatttaa tgccactgaa gtattcttca
      781 ttcaatttac aatcgttgta ttctgtatgg cagttggaag ttatttcagt catttgttta
      841 cagctcaaac agggattaat gttccaattt acgttggctc attatttgta gctgttatcg
      901 tccgaaatat ctctgaaagt tttaatttta atattgtaga tttaaaaatt actaatcaaa
      961 ttggcgatgt cgcattaggt attttcttat ctcttgcgct aatgagcatt caattaatcg
     1021 aaatttataa acttgctata cctcttatta ttatcgtttt agttcaagtt gtcgttatga
     1081 ttttatttgc tgttttaatt ttatttagag gtttaggaaa agattatgat gctgcagtaa
     1141 tggtaggtgg ttttatcggg catgggcttg gtgcaacgcc aaatgccatg gcaaatttag
     1201 atgttattac taaaaaatat ggaaactcac ctaaagcata tttagttgta cctattgttg
     1261 gtgcattctt aatcgattta attggtgtta tagtcattat gggattcatt caatggttta
     1321 gttaaacacc aaactcataa ataaaagagg aggcctccgc ctcctctttt atttatcctc
     1381 gatgtatatt caagctacgt tgttctatcc atgacattat ttccggacta aatacgattt
     1441 gtttttgtgt taagtcgtca atatttttag catctaacat cgtcattatt gatttcatgt
     1501 gttcaataaa tgattctaca taagctactg tatgtgcaat gccattattt tcaacttgat
     1561 ttaaaaacgg acgtgacata ccagttgcct ttgcaccaag tgctaaactt ttaattgcat
     1621 cgagtggtgt acgtaaacca ccactcgcga aaactgaaat ttcgctttga taagccgttg
     1681 tttcaagtaa tgactcaact gtagactgtc cccatgatga taagtaatcc atatctttat
     1741 ttgcacgacg ttcattttca atatctacaa agttagtacc acctttgcca ctaacatcga
     1801 catacttgac gcctatttgt tgtaagtcat gcattaattc tttgctcata ccaaatccaa
     1861 cttcttttat aatgactgga acagacactc gtgatacaat cgacgctata ttatctaacc
     1921 aagtcacaaa ttcacgattc ccttcaggca taactaattc ttgaggagaa ttaacatgga
     1981 tttgtaacgc ttgtgcctca agtaattcaa ctgcttccaa agccttttct actggtacgt
     2041 ccgcaccaac attgctaaaa atcatgcctt caggattcat ttttcgcgca atcgtaaacg
     2101 tctcagccat gcgtggattt ctcaatgccg catgtgttga tccaactgcc atcgctaagc
     2161 gagtttctct tgcaactaca gctagctttt cattgatgtt tttcgtccac tcgctaccac
     2221 ccgtcattgc attaatataa atcggatatg ccatcgttaa gtcaggcgtc tgtgatgtca
     2281 aatcgatatc atttacatta attgatggga tagaatgatg cacaaaacgc atcttatcaa
     2341 aatctgaatg cattgcgtca gattgggcca ttgctatttc aacatgttca ttttttctct
     2401 gttctctttg aaaatcactc atgattaaac ctaccttttc gtcatttcta atttaaaacc
     2461 attttttacg tctgaaaaag atgattaata cgacagctat aacaaacatt acagctaagc
     2521 aaatataata tccataatgt aaatgtaatg ccggcatatt tacaaagttc ataccataaa
     2581 tcccagctat gaatgttaac ggtgaaaata taactgatac taacgttaat acttgcataa
     2641 tactattcat tctaaatgac gtgtatgact caaaattttc tcgtatttcg tttgtcattt
     2701 cttgagcagt acgaatgata ttacgttgct taatcaagtg gtcatcgata tgttgaatgt
     2761 atagcgaatg tttattatct ataatcaaat caccattttg tttcattgta tcaattagct
     2821 cttgcatagg aaacagtaca cgttttactt taatcaaatc cgaacgtaac ttaaagacac
     2881 tatccatgac cattttatta aagcgatcat ctacatggcg gtcttcaaaa tgataaacac
     2941 tatcttcaag tgcatataca aagttgaaat atttatcaac catcatatct aaaattaata
     3001 tgacgacatc tgcacaatct aattctgcat ctaatgtatt catatactta tagactactt
     3061 tatttaatga ttccaacgtt tgatgatgat atgttactaa tacattgtct tgtataaaaa
     3121 tatttagtgc tattggtgaa tagtttgacc ccataatact atgaaatact aagtattgat
     3181 aatctttata agatttatat ttagctcgtg gcataccgtt aattgcatca tccacttcta
     3241 aatcattaaa attaaaatgt gctttaaacc attcattttc ttgttcattc ggttcatcaa
     3301 aatcatacca aacaatagtc gcatcttttg gtatctcttt gatatcatca actactttaa
     3361 acggttcata tgtagtttga taccgtatct ttaaagccat cgatactccc cctaaataac
     3421 gaattctcta ttattttatc atgaattaaa taacgtgtat gtcttaattt attttagtat
     3481 gatagtcact aaggagatgg ttattatcaa acaacttttt acacatactc aaaccgtaac
     3541 atctgaattc attgaccata acaatcatat gcatgatgca aattataata tcatttttag
     3601 tgacgtcgtg aatcgtttta attacagcca cggtctttct ttaaaagaac gcgaaaattt
     3661 agcatataca ctatttacac tagaagaaca tacgacatac ctctcagaat tgtctcttgg
     3721 cgatgtattt actgttactt tatatattta tgattacgat tataagcggt tgcatttatt
     3781 tttaacatta actaaagaag atggtacact agcatcaaca aatgaagtaa tgatgatggg
     3841 aattaatcag cacacacgtc gttctgatgc ttttcctgaa tcattctcaa cacaaatagc
     3901 acactattat aaaaatcaac caactatcac ttggcctgaa caattaggac ataaaatagc
     3961 aattccacac aaaggagcat taaaatgaca gatgcattac aacaaaagat tcatattgaa
     4021 ttactagatt tattagatga tgttaagttt gaattaacag aattaaatgc acaaaaaggg
     4081 ttatacatta acggaccagc aaatcagcta cttaagcgtg gcgttcatat ggcttatgtt
     4141 caaggacaaa agcaagccat cgataatatt atgactattg tggaacagca gcttgaagat
     4201 caacatttcc tagaagatta tgataaattt caaaatgagg ttactcatcg caattatgat
     4261 aaaacagcta actttgcaga acttagtgat attccacgtc aattcgataa ttttttagat
     4321 caattttatc aaattaaagg gcaatacttt atcatcacac atatcaatac acttattggt
     4381 gattttcact cagaagctca ttaacaatta gtctatataa cccttgctat attttcaaaa
     4441 acaaaaccca attacgtttt catgtcaaat atcatcttgc atgaaatcgt aactgggtca
     4501 attgtatgtt attagttatt ttgtgttaca tcctcatcta tcgatttggc aatttgttta
     4561 atagctttat gtgattgtct aattggataa attggaaaat catgtaccat cttaggataa
     4621 tcataaaact caatgtattg atgatgttgc aacatcattt gttcgaatag cttcatatca
     4681 ggatgtgtca tttcacgtcc accaccaaac atataaactg gtggcaatcc ttctattgtg
     4741 ccattaattg gcgatatgcg cttatctgtt aatggtaggc cattcgccca ttttttcata
     4801 atctcattga caccaaactg acttagaacc gcatcttgtt cgattaaggc gtccgaaata
     4861 tctttattag atagtgttgc atctaaaatt ggtgagatta aatacaattt attcggtaat
     4921 ggctgttgat tatctaaaag agattgtaca aaggataatg ccagtgcacc acctgaacca
     4981 tcacccatga ctacgacatt ttgatgtcct acttcagata ctaattgatc ataaacacgt
     5041 tgtatcgctt ggaaagtatc gtcaatatga aactctggtg tctttggata gataggcagt
     5101 acaacctcat ataatgtact taaagtgatt ttatccaaca atctccaatg gaacggtgat
     5161 ggttgtagtg cattgaatcc accgtgaata tataaaattt tcttatcaat ttgatgtctg
     5221 aaattaaagc gaaagacttg catatcatct aatgataatt tttctaaatt tgctttaaca
     5281 tttaatgttg aaggctgctt atgttttttt ctattttcaa tttctctttt ataaaaaaat
     5341 ctttcaacat cttgatcatt tttaaacata atcgagcgat tgtgaagcaa atatttattg
     5401 acaacgctat tcataacacg gtttctaatc aatgtcttaa cctaccttta tatattttat
     5461 gtatccaatg attgtctatc ccctacattc tttgccaaaa aaagtatata atgtagaaga
     5521 tattttcttt ttcactttca aatttaagac tacaattgaa cagtgatttt tcatcattat
     5581 aacagacaac tagacatatt gataagtaaa gaaaagaatt ttatacggag gtgccttgca
     5641 tgacaaatcc aaatcaacga ttagaaccat ttgatgagac atttcaacaa ccgaatattc
     5701 atcgtggtaa gcgatatggt aagaaaaaac gttcattggt aagcatgatt attcaaatca
     5761 ttgttgttat attaaccgca atcgctggat acagcatgtg gaaacaaccc atttttaata
     5821 ttgtcttcac caaagaatct gtagattttg atggcattaa aaattttaac gagacaattg
     5881 gaaaaatgca tcaattaaat attaatttgg gtaatatcaa tgatttacaa caaggaatcg
     5941 atcgcttaat tttagtcttt aacggtttct ttattctatg tatcgttagc ctagtcataa
     6001 caatactgac aattgtattt aatcgcacag ttctaaaagt agtaaatatt ttagtgttaa
     6061 caattatgtt agtcattact ttgtatttca gttatattat taaaacactc gcacaagaaa
     6121 ttgcagatgc tttaaaaaaa tattatttaa atgtaccacc agatcaagtg attacagaag
     6181 ctgacgctat tcataacggc atcattttac tgggctgtag catcggttta cttgttataa
     6241 gcttattctt ccgtaatcgc atgcctcgtt taaaataatg ttatatagta gggattggga
     6301 cagaaatgtt attttcacaa aattcatttc gttgacccac ttcctgcttt ttctttatta
     6361 atgacctact acgtcaatgc caaattttaa tataattcct cttttatttt ataaatcaac
     6421 aaataaaaaa agtatcgata accatatttg gcaccgatac tttaaaaatc atgtccccac
     6481 aacataattt ttgattagtc acacttcaat taatttattt tatttattca tgattgttat
     6541 ttgagcttgc ttctaacttt tgagctgtag cttttgcttt tttattactt tgtaaaaata
     6601 aacataaaat taaagcgatg atgctaaata tcgttgcaac tataaatgca tcattaatac
     6661 cttcaactgt tgctagttta tttacaaatt gtaataacac tttcattgcg ccttcttgac
     6721 caccatattg cgatgctaat tcacgcatat gatcttgtac aacaggattc gttttatcta
     6781 actcttcccc aaaagctgat aaatgttgtg ttgtttgtgt tgtcataaca gtaactaaga
     6841 ttgctgtacc tatagagcct gctaattgac gcatcgtatt taagaaagca ttaccatgag
     6901 aggcaagtcg tcccggtaac gcattaatag ctgcagttac cattggcatc attataaatg
     6961 ccataccaaa tgaacgaagt acatagatac ccatgattgt catatatggt gtatccatat
     7021 ttaatttagt taattcccat gttgcataag tcatgacagc aataccaaag atagctaatg
     7081 gttttaaacc aatagtatct aacaatttac ctgcaaatgg tccaagtaga cccataatta
     7141 gagaaccagg taataataac aatccggaat ctaatgctga gaatccgcgt aaattttgta
     7201 aataaatcgg taataaaatc ataccaccat ataaacttaa cattacaacc atattaataa
     7261 ttgttgttaa tgtaaatgtt gggaatttta atacttctaa attcaacatt ggtgatttca
     7321 ttcttaattc tcttattaca aatagaataa taaagataat accaatcgca aacattgttt
     7381 ctatctcaac agaaccccat cctttgttcc ccgcttctga aaagccgtat aacaaagcac
     7441 caaaaccaat cgtactaaaa atgatacctg ggatatcagc tttagggttt gttgtatatt
     7501 gatataactt aaaccataca aaaccaacta agattgcaat aataccgata atgaacatac
     7561 cgtaaaacat cacattccaa tggtaatttt gtacaatata acctgataat gttggaccaa
     7621 ttgcaggtgc taaaatcatt gcgataccca ttgtacccat ggcagcacca cgtttttcag
     7681 gtggataaat tgtaataata acaattgaac ctaatggcat tagtacacct gcaccaatgg
     7741 cttgtaatac acgtccaacc atcatgattg ggaaattcat tgaaatcgca cagattaatg
     7801 aaccaattgt aaagagtact aacgcaacta aaaataattt tcgatatgaa tatttattaa
     7861 atagatacgc cgtaattggt attaaaatac cgtttactaa catgaatccc gtcatcaacc
     7921 attgccctgt tgacgcagaa atattaaatt ccgtattaat ttttggcaaa gcaacattta
     7981 ataatgtttg gtttaaaatc gcaataaaca taccgaataa taatgccgct aatattttac
     8041 cgcgtgaaac accttcacca aaaataaagt ttttatgttc tttttttatt ttttcattca
     8101 ctttatattc ttctgattca ggatttttag cagcaactgc ttcctcacca ttattattag
     8161 tgaatgcttc ttgatctttc tcagacccct ttgtgtaacc atttagacta acttggctat
     8221 gatcatcttg attgtcaaca cgcaactctt catgcgtcat acgttgtgtc gattgatcag
     8281 ttgttttgtc taaatcacta gctttaaatt tagattgatt tgattgacgt gtcgtaaatt
     8341 gttgttcctt ttgttggcgt ttgccttttt ttcttgatct tattaaaaat aaattgataa
     8401 ccccaacaat aatgagcgct aaaataatgt agctaataat gaaggtcgta gtcatttaat
     8461 gaccccctta atttttatgg atttttactt cagcgttcat tccaggaaca acttgtttag
     8521 acggttctga ttctagagtg attttaacag gtattacttg agaaacttta gtgtagttac
     8581 catcactatt tgatgatggc attaatgaaa agcttgcagc agttgctttt ccaatactat
     8641 caactttacc tttaatagaa gctttttgac cgtcaatagt cacatcaaca tctttaccta
     8701 cttcaacatc tttaatatct ttttcgtcaa tatttgctgt tacatataaa tcatctaaat
     8761 tgtatgcata agcgattggg ttaccagctt gcaccattga accttccata ccatctaatt
     8821 tggcaattgt acctttttga ggcattttaa gatccatatc tttcgtttcg ccatcttgac
     8881 cttgtacagt aacagttgct actttgtcac ctttatcgag tttgtcacct tgtttaacat
     8941 taagtgattt aatttgtcca gatgcaggac ttgctatttt aatttgatcg ccatttactt
     9001 ttgcattatc agttgttaca tagcttgttg ttttattcca gaaataaaag ccagcaatcc
     9061 caattgctaa cagtacaaca accgtaatga cattaattaa tatcatcttt ttcatgaaac
     9121 atttcctcct atttgctttt aaaattcact ttatttatta tagagacatg ataactaaaa
     9181 aaacaccaac aatttttata agagtgttgg ttaaaattca caaaggtgta acaatatgaa
     9241 acgacaagcc aaaatagaaa ttcaaaacgc acttgttgat ttgatggctg aatatccttt
     9301 tcaagaaata tccactaaaa tgatttgcgc gtattgtaat attaatcgtt ctacttttta
     9361 tgattattac aaagataaat ttgatttatt ggataccatc aactcaaagc ataaagaaaa
     9421 attccaattt cttctaagcg cgctacatca taatttcgaa aatattaaac aggataagct
     9481 taaactttat aaattcttca ttattattgc taagtatatc aagcacaatg agcagttttt
     9541 caaagacatt ttagttacct atccaatgaa aacattattc atagactata taaatttagc
     9601 gagagattac tatcaacaaa ttatgaatga ctatagtact acagtaacca ataaacagct
     9661 ttatgttaca tatatcatag gcggtcaagc tggggtgttt attaattggc tttcgaatgg
     9721 ttgtaatgaa tcacctgaag aagttgctga cattttattg gctaatacga ttaaacttca
     9781 aatccaataa tacggtttca ttctaataaa gaaatgctat aaaacaacta gataaagtta
     9841 aaccaaatgg atagataata gtacatatgt tacggtgcta tcaatataaa aatgccgaga
     9901 tttccacata ataatctcgg catttttatt tttgatgcta aattcatagg tctatgaata
     9961 gctcaagttt tgttttttaa tttttcaatt caatataata gaagaaacta tgtttatcat
    10021 cgatttgttt t
//
LOCUS       DANBWH010000096         9939 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.099, whole genome shotgun sequence.
ACCESSION   DANBWH010000096 DANBWH010000000
VERSION     DANBWH010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9939
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.099"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..74)
                     /locus_tag="PPY92_001613"
     rRNA            complement(<1..74)
                     /locus_tag="PPY92_001613"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(196..271)
                     /locus_tag="PPY92_001614"
     tRNA            complement(196..271)
                     /locus_tag="PPY92_001614"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(236..238),aa:Ala,seq:tgc)
     gene            complement(295..368)
                     /locus_tag="PPY92_001615"
     tRNA            complement(295..368)
                     /locus_tag="PPY92_001615"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(332..334),aa:Pro,seq:tgg)
     gene            complement(389..465)
                     /locus_tag="PPY92_001616"
     tRNA            complement(389..465)
                     /locus_tag="PPY92_001616"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(429..431),aa:Arg,seq:acg)
     gene            complement(471..559)
                     /locus_tag="PPY92_001617"
     tRNA            complement(471..559)
                     /locus_tag="PPY92_001617"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(523..525),aa:Leu,seq:taa)
     gene            complement(567..641)
                     /locus_tag="PPY92_001618"
     tRNA            complement(567..641)
                     /locus_tag="PPY92_001618"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(607..609),aa:Gly,seq:gcc)
     gene            complement(675..747)
                     /locus_tag="PPY92_001619"
     tRNA            complement(675..747)
                     /locus_tag="PPY92_001619"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(712..714),aa:Lys,seq:ttt)
     gene            complement(754..829)
                     /locus_tag="PPY92_001620"
     tRNA            complement(754..829)
                     /locus_tag="PPY92_001620"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(794..796),aa:Thr,seq:tgt)
     gene            complement(846..921)
                     /locus_tag="PPY92_001621"
     tRNA            complement(846..921)
                     /locus_tag="PPY92_001621"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(886..888),aa:Val,seq:tac)
     gene            complement(934..1048)
                     /gene="rrf"
                     /locus_tag="PPY92_001622"
     rRNA            complement(934..1048)
                     /gene="rrf"
                     /locus_tag="PPY92_001622"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(1606..3093)
                     /gene="lysS"
                     /locus_tag="PPY92_001623"
     CDS             complement(1606..3093)
                     /gene="lysS"
                     /locus_tag="PPY92_001623"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDJ2625618.1"
                     /translation="MSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEW
                     DQYSKEELVEKEADSHVAIAGRLMTKRGKGKAGFAHVQDLAGQIQIYVRKDQVGDDEF
                     DLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTKSLRPLPDKFHGLQDIEQRYR
                     QRYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTH
                     HNALDATLYMRIAIELHLKRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAY
                     ADYHDIMDLTESMVRHIANEVLGSAKVQYNGETIDLESAWTRLHIVDAVKEATGVDFY
                     EVKSDEEAKALAKEHGIEIKDTMKYGHILNEFFEQKVEETLIQPTFIYGHPTEISPLA
                     KKNPEDPRFTDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDE
                     DYIEALEYGMPPTGGLGIGIDRLVMLLTDSPSIRDVLLFPYMRQK"
     gene            complement(3146..3238)
                     /locus_tag="PPY92_001624"
     CDS             complement(3146..3238)
                     /locus_tag="PPY92_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625619.1"
                     /translation="MVNDKVLETSKEMYVEQKCLIFYKTLKENV"
     gene            complement(3632..4108)
                     /gene="folK"
                     /locus_tag="PPY92_001625"
     CDS             complement(3632..4108)
                     /gene="folK"
                     /locus_tag="PPY92_001625"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDJ2625620.1"
                     /translation="MIQAYLGLGSNIGDRESQLNDAIKILNEYDGISVSNISPIYETA
                     PVGYTEQPNFLNLCVEIQTTLTVLQLLECCLKTEECLHRIRKERWGPRTLDVDILLYG
                     EEMIDLPKLSVPHPRMNERAFVLIPLNDIAANVVEPRSKLKVKDLVFVDDSVKRYK"
     gene            complement(4105..4470)
                     /gene="folB"
                     /locus_tag="PPY92_001626"
     CDS             complement(4105..4470)
                     /gene="folB"
                     /locus_tag="PPY92_001626"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HDJ2625621.1"
                     /translation="MQDTIFLKGMRFYGYHGALSAENEIGQIFKVDVTLKVDLAEAGR
                     TDNVIDTVHYGEVFEEVKSIMEGKAVNLLEHLAERIANRINSQYNRVMETKVRITKEN
                     PPIPGHYDGVGIEIVRENK"
     gene            complement(4448..5251)
                     /gene="folP"
                     /locus_tag="PPY92_001627"
     CDS             complement(4448..5251)
                     /gene="folP"
                     /locus_tag="PPY92_001627"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDJ2625622.1"
                     /translation="MTKTKIMGILNVTPDSFSDGGKFNNVETAINRVKAMIDEGADII
                     DVGGVSTRPGHEMVTLEEELNRVLPVVEAIVGFDVKISVDTFRSEVAEACLKLGVDMI
                     NDQWAGLYDHRMFQIVAKYDAEIILMHNGNGNRDEPVVEEMLTSLLAQAHQAKIAGIP
                     SNKIWLDPGIGFAKTRNEEAEVMARLDELVATEYPVLLATSRKRFTKEMMGYDTTPVE
                     RDEVTAATTAYGIMKGVRAVRVHNVELNAKLAKGIDFLKENENARHNLS"
     gene            complement(5467..6399)
                     /gene="cysK"
                     /locus_tag="PPY92_001628"
     CDS             complement(5467..6399)
                     /gene="cysK"
                     /locus_tag="PPY92_001628"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDJ2625623.1"
                     /translation="MAQKPVDNITQIIGGTPVVKLRNVVDDNAADVYVKLEYQNPGGS
                     VKDRIALAMIEKAEREGKIKPGDTIVEPTSGNTGIGLAFVCAAKGYKAVFTMPETMSQ
                     ERRNLLKAYGAELVLTPGSEAMKGAIKKAKELKEEHGYFEPQQFENPANPEVHELTTG
                     PELLQQFEGKTIDAFLAGVGTGGTLSGVGKVLKKEYPNIEIVAIEPEASPVLSGGEPG
                     PHKLQGLGAGFIPGTLNTEIYDSIIKVGNDTAMEMSRRVAKEEGILAGISSGAAIYAA
                     IQKAKELGKGKTVVTVLPSNGERYLSTPLYSFDD"
     gene            complement(6578..7459)
                     /gene="hslO"
                     /locus_tag="PPY92_001629"
     CDS             complement(6578..7459)
                     /gene="hslO"
                     /locus_tag="PPY92_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDJ2625624.1"
                     /translation="MTHDYIVKALAFDGEIRAYAALTTETVQEAQTRHYTWPTASAAM
                     GRTMTATAMMGAMLKGDQKLTVTVDGQGPIGRIIADANAKGEVRAYVDHPQTHFPLNE
                     QGKLDVRRAVGTNGSIIVVKDVGMKDYFSGASPIVSGELGEDFTYYYATSEQTPSSVG
                     LGVLVNPDNTIKAAGGFIIQVMPGAKDETISKLEKAISEMTPVSKLIEQGLTPEGLLN
                     EILGEDHVQILEKMPVQFECNCSHEKFLNAIKGLGEAEIQNMIKEDHGAEAVCHFCGN
                     KYKYTEEELNVLLESLA"
     gene            complement(7687..9780)
                     /gene="ftsH"
                     /locus_tag="PPY92_001630"
     CDS             complement(7687..9780)
                     /gene="ftsH"
                     /locus_tag="PPY92_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDJ2625625.1"
                     /translation="MQKAFRNVLVIVIIGVIIFGLFSYLNGNGNMPKQLTYNQFTEKL
                     EKGDLKTLEIQPQQNVYMVSGKTKNDEDYSSTILYNNEKELQKITDAAKKQNGVKLTI
                     KEEEKQSVFVSILSTLIPVVVIALLFIFFLSQAQGGGSGGRMMNFGKSKAKMYDNNKR
                     RVRFSDVAGADEEKQELIEIVDFLKDNKKFKEMGSRIPKGVLLVGPPGTGKTLLARAV
                     AGEAGAPFFSISGSDFVEMFVGVGASRVRDLFDNAKKNAPCIIFIDEIDAVGRQRGAG
                     VGGGHDEREQTLNQLLVEMDGFGENEGIIMIAATNRPDILDPALLRPGRFDRQIQVGR
                     PDVKGREAILHVHAKNKPLDETVDLKAISQRTPGFSGADLENLLNEASLIAVREGKKK
                     IDMRDIEEATDRVIAGPAKKSRVISKKERNIVAHHEAGHTIIGMVLDEAEVVHKVTIV
                     PRGQAGGYAMMLPKQDRFLMTEQELLDKICGLLGGRVSEDINFNEVSTGASNDFERAT
                     QIARSMVTQYGMSKKLGPLQFGHSNGQVFLGKDMQGEPNYSSQIAYEIDKEVQRIVKE
                     QYERCKQILLEHKEQLILIAETLLTEETLVAEQIQSLFYEGKLPEIDYDAAKVVKDED
                     SEFNDGKFGKSYEEIRKEQLEDGQRDESEDRKEEKDIAEDKKEADKSDEKDEPAHRQA
                     PNIEKPYDPNHPDNK"
BASE COUNT         2979 a         2112 c         1391 g         3457 t
ORIGIN      
        1 tccgttcgct cgacttgcat gtattaggca cgccgccagc gttcatcctg agccaggatc
       61 aaactctcca taaaaattat gatgtttgat tagctcataa atactaaata atgtttgtaa
      121 cttatagtta cgttttttgg aattaacgtt gacatattgt cattcagttt tcaatgttca
      181 tcgtgtaaaa ataaatggtg gagactagcg ggatcgaacc gctgacctcc tgcgtgcaaa
      241 gcaggcgctc tcccagctga gctaagcccc cataaaatga aatttaagaa gtaatcggga
      301 agacaggatt cgaacctgcg accccttggt cccaaaccaa gtgctctacc aagctgagct
      361 acttcccgtt attcaattaa tttaaaaatg gcgcgcccga taggagtcga acccataacc
      421 tcttgatccg tagtcaaacg ctctatccaa ttgagctacg ggcgctaaaa tggtgccgag
      481 gaccggaatc gaaccggtac ggtgatctct caccgcagga ttttaagtcc tgtgcgtctg
      541 ccagttccgc caccccggca aaataatgga gcagaagacg ggattcgaac ccgcgacccc
      601 aaccttggca aggttgtatt ctaccgctga actacttctg cattattttg ctaaatataa
      661 aaattaaatc gtaatgagcc atagaggatt cgaacctctg accctctgat taaaagtcag
      721 atgctctacc agctgagcta atggctcaaa agatggtgcc ggccagagga cttgaacccc
      781 caacctactg attacaagtc agttgctcta ccaattgagc taggccggct atatctaaga
      841 ataaatggtg gagaatgacg ggttcgaacc gccgaccctc tgcttgtaag gcagatgctc
      901 tcccagctga gctaattctc cgatttgtca tttgcctggc aacgttctac tctagcggaa
      961 cgtaagttcg actaccatcg acgctaagga gcttaacttc tgtgttcggc atgggaacag
     1021 gtgtgacctc cttgctatag ccaccagaca aaataactta cttatttatg atacacgctt
     1081 aattaataag gtgcaatagt taattttaca ctttgttgtg aactttgtca catcagcttt
     1141 tagtgtgttt cgtctgtttt tgacgacttt tatatattac ttcattcaat tgataaagtc
     1201 aacactttta atcaactttt ttattcttca aaactgacat ttctcgtgct ctctaattca
     1261 taacactttt agtattctat catacacata tgtacaaaac catacacatt ctatacaatt
     1321 ttaaagtact tttatagctg attaagataa attatactaa tatagcaatt tttgttataa
     1381 gatatctcaa tgtctatcgt taacatttct catccgaact actctatata cgtatcgtca
     1441 ttttcatcta tcagccaact accttgttta acagtcaaaa atagtaccac tatcgttaac
     1501 tattactatc aaaaacattc aaacacgaac taattaccat aattaagaaa accctcgcat
     1561 acacatgttg tatacacgag agctcttact aacaatcaac gtcatttatt tttgtctcat
     1621 ataagggaat aataacacgt ctctgattga tggagaatca gttaataaca taaccaatct
     1681 gtcaataccg ataccaagac cacctgtcgg aggcatacca tattctaacg cttcaatgta
     1741 atcttcatcc atttcatgcg cttcatcatt accttgcgcc ttttcaacaa gttgcgcttc
     1801 aaaacgacct ttttgatcaa taggatcatt taattcagta aatgcatttg catgctctct
     1861 acctacaatg aacaattcga aacgatcagt aaatctagga tcttcaggat ttttcttcgc
     1921 taaaggtgaa atttcagtcg gatgaccata gataaacgtt ggctgaataa gtgtttcttc
     1981 aactttttgc tcaaagaatt catttaaaat atgaccatat ttcattgtat ctttaatttc
     2041 aataccatgt tctttagcta aagctttagc ttcttcatca cttttaactt cataaaaatc
     2101 cacaccagta gcttctttta cagcatcaac aatatgcaaa cgagtccaag cagattctaa
     2161 atcaatcgtt tccccattgt attgtacttt tgcagaacct aatacttcat tggcaatatg
     2221 tctcaccata gattctgtta aatccataat gtcatgataa tctgcatatg cttcatataa
     2281 ttcaatcatt gtgaattcag ggttatgtct agttgataca ccttcattac ggaatactct
     2341 accaatttca tatacttttt caagtccacc gacaattaaa cgttttaaat gcaactcaat
     2401 agcaatacgc atgtataacg ttgcatctaa tgcattatga tgtgttacaa atggtctagc
     2461 agctgcgcca ccagcaattt ggtgcatcat aggtgtttct acttccaaga aacctttatt
     2521 atttaaataa ttacgcattt cttgaatgat tttactacga ttaataaatg tacgagtgct
     2581 atcttcgttc gtaattaaat ctaaatatct ttgacgatat ctctgttcaa tatcctgtaa
     2641 accgtggaat ttatccggta atggtcgcaa tgatttagtt agtagcgtga atttcttcgc
     2701 tttaaccgat aattcgccag tatttgtttt gaacattaca ccttcaacac caacgatatc
     2761 gcctaaatca gcatttttcc ataaatcaaa ttcgtcatcg ccaacttgat ctttacgaac
     2821 gtaaatttga atttgtccag ctaagtcctg aacgtgtgca aatcctgctt tacctttacc
     2881 acgcttagtc attaatcgtc cagctatagc gacatgacta tccgcttctt tttctaccaa
     2941 ttcttcttta gaatactggt cccactcttc tttcaaatca ctagataaac ctgaacggtc
     3001 aaatttagaa ccaaacgggt ctataccaag atcatataat tcttgtaatt tttgacgtcg
     3061 aaccaacatt tggtcattca tttcttctga cataactttc tctcctttaa cttgttgtaa
     3121 tcctttagta ttccaactgg tacactcaaa cattttcctt taaagtttta taaaatatca
     3181 gacatttttg ctcaacatac atctctttcg atgtttctaa tactttatca ttaaccatta
     3241 caccttaaag ttatgatgtg gcatgttttc ttatattcat aacatcaatt ttatcacttt
     3301 tttaatttta caagagcttg taaatgtaat aaagcaattt ctgttttcgt ttatgaatta
     3361 cttattcatt taaaatacga ttatcattaa tacaacccta agtaaatgta taaaaattat
     3421 cttccacaaa cttcaacaaa agcccaaata aattacagca atttatcaaa tattgcttac
     3481 tttgatttta tgaaacaact taattctaac acatactaaa tcatcatata ctaattcgta
     3541 atcaaatgca tttagagata atcaaaatgc ggaaacatct ccaataatca atcatctatt
     3601 cccaataaat atgaatgttc tcaacaatac attatttata tctctttaca ctgtcatcga
     3661 caaaaactaa atctttcact ttcaatttcg aacgtggttc tacgacattt gctgctatat
     3721 catttaatgg gattaaaaca aatgcacgtt cattcattct cggatgtggc accgacagtt
     3781 ttggtaaatc tatcatttct tctccataca acaaaatatc cacatctaaa gttctaggac
     3841 cccatcgttc ctttctaata cggtgtaaac attcttctgt cttcaaacaa cattccaaca
     3901 gttgtaatac tgtgagtgtt gtttgaattt caacacacaa atttaaaaag ttaggttgct
     3961 cagtataccc aactggtgct gtttcataaa tcggagaaat attagatacg ctaataccat
     4021 catattcatt caaaatcttt atagcatcgt ttaactggct ttctctatca ccaatattac
     4081 tacctaatcc taagtatgct tgaatcattt attctccctc actatttcga tacctactcc
     4141 atcataatga cccggaatcg gtgggttttc tttagtgatt ctcacttttg tttccattac
     4201 acgattatat tgtgaattta tacgatttgc aatacgttca gctagatgct caagtaaatt
     4261 aacggcctta ccttccataa ttgatttaac ctcttcgaac acttcaccat aatgaactgt
     4321 atcaataaca ttatcagtac gcccagcttc agctaagtct actttcaaag ttacatccac
     4381 tttgaaaatt tgccctattt cattttcagc tgataaagca ccatgatatc cataaaagcg
     4441 cataccttta agaaagattg tgtcttgcat tttcattctc ctttaaaaaa tctatacctt
     4501 tagctaattt agcattcaac tcgacattat gaacgcgtac tgctctaacg cctttcataa
     4561 taccatatgc agtcgtagct gcagttactt catctctttc aaccggtgtt gtatcataac
     4621 ccatcatctc tttagtgaaa cgtttccggc ttgtcgctaa taaaactgga tattctgttg
     4681 caacaagttc atccagtctt gccataactt cggcttcttc atttctagtt ttagcgaaac
     4741 ctatacctgg atctagccaa attttatttg aaggtatacc agctatttta gcttgatgtg
     4801 cttgtgctaa caaagatgtt aacatttctt cgacaaccgg ttcatcacga ttaccatttc
     4861 cattatgcat taaaataatt tccgcgtcat atttagctac aatttggaac atacgatgat
     4921 catacagacc cgcccattga tcattaatca tatcaacgcc taattttaaa catgcttcag
     4981 caacctcact tcgaaatgta tcgactgaaa tttttacatc aaaaccgaca atagcttcaa
     5041 caacaggtaa tactctgttc agctcttctt ctaatgtaac catttcatga ccgggtctcg
     5101 ttgaaacacc tccaacatct ataatgtcag caccttcatc tatcatggct ttcactctat
     5161 ttatagctgt ttcaacatta ttaaattttc caccatctga gaatgaatca ggtgtgacgt
     5221 ttaatatgcc cataattttt gttttagtca tgttaaccac cctttaatta tgtattgttt
     5281 aactctaata aaatttactc ctgcgattta ctagttatta atatgtacgt ttattatctt
     5341 atcattcact ggatgttttt atcagaaaaa tgtttaattt aacgctcata tttttaaaat
     5401 tatacatggt agaaaaaaag agcaagttat ctcaaaaaga taactcactc ttttaaatga
     5461 cattaattag tcatcgaatg aatataaagg tgttgataag tagcgttcac cattactcgg
     5521 caatactgtt actactgttt taccttttcc taattctttt gctttttgaa tggcagcata
     5581 aatcgcagca cctgatgaaa tacctgctaa aataccttcc tctttagcaa ctcgacgaga
     5641 catttccatc gctgtatcat ttcctacttt aataatactg tcatagattt ctgtattcaa
     5701 agtgcctgga ataaatccag cacctaaacc ttgtaattta tgtggacctg gctcaccacc
     5761 gctcaatact ggagaagcct caggctctat agcaacaatt tcgatgttag gatattcttt
     5821 tttcagaact ttacctacac cagataacgt accaccagta ccaacaccag ctaggaacgc
     5881 atcgatagtt ttcccttcaa attgttgtaa taactcagga cctgtagtta actcatgaac
     5941 ttcagggttc gcagggtttt caaattgttg tggctcgaag taaccatgtt cttcttttaa
     6001 ttctttagct tttttaattg cacctttcat cgcttctgat ccaggcgtta aaactaattc
     6061 cgcaccgtat gcttttaata aattacgacg ctcttggctc attgtttcgg gcatagtaaa
     6121 tactgcttta tatcctttag cagcacatac aaatgctaaa ccgatacctg tattaccact
     6181 tgttggttct acaattgtgt cgccaggttt aattttgcct tctcgctctg ctttttcaat
     6241 cattgctaaa gcaattctat cctttacaga accacctgga ttttgatatt ccaattttac
     6301 ataaacatct gctgcattgt catctactac atttctcaat ttgactaccg gtgtaccgcc
     6361 aataatttga gtaatattat ctactggttt ttgtgccatt aagaacactc ctttttagtt
     6421 agttgttgaa atcttagttt atttatcgga tatagaatta ttttatcaaa taaattctag
     6481 aaagcaaaca aaatcactaa ggattataca actatttagt tctacatttc actaaaccgc
     6541 taaaaataaa cattttagcg tattgattta aattaaatta cgctaaactt tctagcaaca
     6601 cgtttaattc ttcttcagta tatttatatt tatttccaca gaaatgacat actgcttcag
     6661 caccatgatc ttctttaatc atattttgaa tctcagcctc gcccaatcct ttaatagcat
     6721 ttaaaaattt ctcatgacta caattacatt caaattgaac aggcattttc tctaaaattt
     6781 gcacatggtc ttcacctaag atttcgttta gtaatccttc tggcgttaat ccttgttcaa
     6841 ttaatttaga aactggtgtc atttcactaa ttgctttttc taattttgaa attgtttcat
     6901 ctttggcacc tggcataact tgaatgataa atcctcctgc tgctttaatc gtattatcag
     6961 gatttaccaa tacaccaaga cctaccgatg aaggtgtttg ttcacttgta gcataataat
     7021 aagtaaaatc ttcaccaagt tctcctgaaa caattggact tgctccagag aaatagtctt
     7081 tcattccaac gtctttaaca actataatag atccatttgt ccctactgct cgtcttacat
     7141 caagtttacc ttgctcattt aatggaaaat gagtttgtgg atggtctaca taagcacgca
     7201 cctcgccttt agcatttgca tcggcaataa ttcgtccaat aggtccttgg ccatctacag
     7261 tgacagttaa tttttgatca cctttcaaca ttgcgcccat catagctgtt gctgtcattg
     7321 ttcttcccat tgcagcagat gctgtcggcc atgtataatg tctcgtttgt gcttcttgaa
     7381 cagtttcagt tgtcaaagca gcataagccc taatctcccc atcaaatgct aatgctttaa
     7441 caatataatc gtgtgtcatt atttcaatct cctctattac tctatatttt aaaattactt
     7501 tacttcataa aatgcaacaa ttgtacttat tctacaccca tcattctaaa taatgaagta
     7561 acttgtttta caattatttt ctgctataac aattcaatga cttaaaatct aatacgtatt
     7621 tttaaaaacg ataaaagtac ctctttctat aactttatca tagaaagagg tactgaatat
     7681 aatcgattat ttattgtctg ggtgatttgg atcgtaaggt ttttcgatat ttggggcttg
     7741 tcgatgtgct ggttcatctt tttcatcaga tttatcagct tcttttttat cctcagcaat
     7801 atctttttct tctttacgat cttcactttc gtcacgttgt ccatcttcta attgctcttt
     7861 acgaatctct tcataagatt taccgaattt accatcatta aattcagaat cttcatcttt
     7921 aacaacttta gctgcatcat aatcaatttc aggtaattta ccttcgtaga ataatgattg
     7981 aatttgttca gcaactaatg tttcttctgt taataatgtt tcagcaatta aaattaattg
     8041 ttctttgtgc tctaataaaa tttgtttaca acgttcgtat tgttctttaa cgattcgttg
     8101 aacttcttta tcaatttcat atgcgatttg gcttgaataa ttaggctcac cttgcatatc
     8161 tttacctaag aatacttgac cattgctatg accgaactgt aatggtccta attttttact
     8221 cataccatat tgcgtaacca ttgagcgtgc gatttgtgtt gcacgttcga agtcatttga
     8281 agcacctgtt gatacttcgt taaagttaat atcttctgat acacgtccac caagtaaacc
     8341 acagatttta tctaataact cttgttcagt cattaagaaa cgatcttgtt taggtagcat
     8401 cattgcataa ccacctgctt gtccacgtgg aacaatagta actttatgca ctacttctgc
     8461 ctcatcaagt accataccga taattgtatg accagcttcg tgatgagcaa caatattacg
     8521 ttctttctta gaaataactc gagatttctt agcaggtccg gctataactc tatccgttgc
     8581 ctcttcgata tctctcatgt caattttctt tttaccttca cgtacagcaa ttaaagatgc
     8641 ttcatttaat aagttctcta aatcagcacc tgagaaacca ggtgtacgtt gtgaaattgc
     8701 ttttaaatca accgtttcat caagtggttt gtttttagca tgaacatgaa gaattgcttc
     8761 acggcctttc acatctggac gaccaacttg aatttgtcta tcaaaacgac ctggacgtaa
     8821 taaggctggg tcaaggatat caggacggtt tgtagcagct atcataatga taccttcatt
     8881 ttcaccgaaa ccatccattt caactaataa ttggtttagg gtttgttcac gttcatcatg
     8941 accgccacca acacctgcac cacgttgacg accaacagca tcaatctcat cgataaagat
     9001 gatacaaggc gcgtttttct tagcattatc gaataagtca cgaacacggc tcgcaccaac
     9061 accaacaaac atctctacaa agtctgaacc actaatagag aagaatggtg cgccagcttc
     9121 acctgcaacc gctctagcaa gtaatgtttt accagtacct ggaggtccaa caagtaagac
     9181 acctttagga atcctagatc ccatttcttt gaatttttta ttatctttca agaaatcaac
     9241 aatttcaatt aattcttgtt tttcttcatc tgcccctgct acatcagaga aacgaacacg
     9301 acgtttatta ttatcgtaca tttttgcttt agatttacca aagttcatca tacgaccgcc
     9361 actaccgcca ccttgtgctt ggcttaggaa gaaaataaat aataacgcta tgactacaac
     9421 tggaattaat gttgaaagta tactcacaaa gacactttgt ttttcttctt ctttaatcgt
     9481 taattttaca ccgttttgct ttttagcagc atcagtaatt ttttgtaatt ctttttcgtt
     9541 gttatataaa atagttgatg aatagtcttc atcatttttc gttttaccac ttaccatata
     9601 gacattttgt tgtggttgga tttctaaagt tttaaggtca cctttttcca acttctcagt
     9661 aaattgatta tatgtaagct gtttcggcat atttccatta ccgtttaaat atgaaaatag
     9721 accaaaaata ataacgccta ttattacgat aactagcaca ttgcgaaaag ctttctgcat
     9781 gcgtcatttc ctcctacttc ccataataaa actaacaaaa attgtaacat atgacatgtt
     9841 ttataagcta gtaattgagc ttaacatcaa tgtcattcac acccgtatta gttttatcaa
     9901 tctctatatt tttcaacttt tcaagacata tgtcttgtc
//
LOCUS       DANBWH010000097         9855 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.100, whole genome shotgun sequence.
ACCESSION   DANBWH010000097 DANBWH010000000
VERSION     DANBWH010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9855
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.100"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            114..1427
                     /gene="murT"
                     /locus_tag="PPY92_001631"
     CDS             114..1427
                     /gene="murT"
                     /locus_tag="PPY92_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HDJ2625626.1"
                     /translation="MRQWTAIHLAKLARKASRAVGKRGTDLPGQIARKVDTDVLRKLA
                     EQVDDIVFISGTNGKTTTSNLIGHTLKANNIQIIHNNEGANMAAGITSAFIMQSTPKT
                     KIAVIEIDEGSIPRVLKEVTPSMMVFTNFFRDQMDRFGEIDIMVNNIAETISNKGIKL
                     LLNADDPFVSRLKIASDTIVYYGMKAHAHEFEQSTMNESRYCPNCGRLLQYDYIHYNQ
                     IGHYHCQCGFKREQAKYEISSFDVAPFLYLNINDEKYDMKIAGDFNAYNALAAYTVLR
                     ELGLNEQTIKNGFETYTSDNGRMQYFKKERKEAMINLAKNPAGMNASLSVGEQLEGEK
                     VYVISLNDNAADGRDTSWIYDADFEKLSKQQIEAIIVTGTRAEELQLRLKLAEVEVPI
                     IVERDIYKATAKTMDYKGFTVAIPNYTSLAPMLEQLNRSFEGGQS"
     gene            1429..2160
                     /locus_tag="PPY92_001632"
     CDS             1429..2160
                     /locus_tag="PPY92_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDJ2625627.1"
                     /translation="MHELTIYHFMSDKLNLYSDIGNIIALRQRAKKRNIKVNVVEINE
                     TEGITFDECDIFFIGGGSDREQALATKELSKIKTPLKEAIEDGMPGLTICGGYQFLGK
                     KYITPDGTELEGLGILDFYTESKTNRLTGDIVIESDTFGTIVGFENHGGRTYHDFGTL
                     GHVTFGYGNNDEDKKEGIHYKNLLGTYLHGPILPKNYEITDYLLEKACERKGIPFEPK
                     EIDNEAEIQAKQVLIDRANRQKKSR"
     gene            2246..2356
                     /locus_tag="PPY92_001633"
     CDS             2246..2356
                     /locus_tag="PPY92_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625628.1"
                     /translation="MDGNNQIDVCFFVLVNNYYTMSIYPRIINSNGDYAT"
     gene            2385..2798
                     /locus_tag="PPY92_001634"
     CDS             2385..2798
                     /locus_tag="PPY92_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625629.1"
                     /translation="MKPTKVILRDASYLHSKTSITFILKDVVIEEDNKIYYFDTSATF
                     EDQEVKFEFALFDSDMDNLKHMDYDNPVTEIYFIEPDLHFTIIDFNQELLCIYIDFDS
                     GLRHSNMATESGISLRINIAKSDFTKFINELASLY"
     gene            complement(3229..4215)
                     /locus_tag="PPY92_001635"
     CDS             complement(3229..4215)
                     /locus_tag="PPY92_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDJ2625630.1"
                     /translation="MNDQWYKHLIGARTIKTGIAIFLTAVFCMALDLTPIYAILTAVV
                     TIEPTAKASLIKGYRRLPATVIGAGFAVLFTYLFGDQSPFTYALSATFTILFCTKLKL
                     QVGTNVAVLTSLAMIPGIHDAYIFNFLSRTLTAIIGLVTSGLINFMVFPPKYYGQVEE
                     KLSKTDALMYKLFYNRCQELILSRLQSDKSEKAYKNIFNLNNQVETLISYQRDELSYH
                     KKKECDWKLLNQLTKRAYTNRLFITHLSNIIYLPKNTRVNFSGDEKMALLKISSSIKD
                     IFYDGTFKREDDSVETLRSTIKALEISGENQIKSHILYEVLMIYRLLDSRYA"
     gene            4746..5504
                     /gene="map"
                     /locus_tag="PPY92_001636"
     CDS             4746..5504
                     /gene="map"
                     /locus_tag="PPY92_001636"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDJ2625631.1"
                     /translation="MIVKTEEELQALKEIGYICAKVRNTMQAATKPGITTKELDNIAK
                     ELFEEYGAISAPIHDENFPGQTCISVNEEVAHGIPSKRVIREGDLVNIDVSALKNGYY
                     ADTGISFVVGESDDPMKQKVCDVATMAFENAIAKVKPGTKLSNIGKAVHNTARQNDLK
                     VIKNLTGHGVGLSLHEAPAHVLNYFDPKDKTLLTEGMVLAIEPFISSNASFVTEGKNE
                     WAFETSDKSFVAQIEHTVIVTKDGPILTTKIEEE"
     gene            5721..6107
                     /locus_tag="PPY92_001637"
     CDS             5721..6107
                     /locus_tag="PPY92_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625632.1"
                     /translation="MNYVERYIEQFLRATVRNNIKHYLLMLDEKMKNLDDYMRYLITK
                     KEQLSKLIDSLMLTLENKYIDIAEAFQIQCAREINNQEIENIKSELNKVEAYYAQIET
                     QIQQTSTEKIATEKTSYLINYMNAVA"
     gene            6122..6823
                     /locus_tag="PPY92_001638"
     CDS             6122..6823
                     /locus_tag="PPY92_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HDJ2625633.1"
                     /translation="MTHKYISTQMLIIFTALMIIANFYYIFFEKIGFLLVLLLGCVLV
                     YVGYLYFHKIRGLLAFWIGALLIAFTLLSNKYTIIILFVFLLLLIVRYLIHKFKPKKV
                     VATDEVMTSPSFIKQKWFGEQRTPVYVYKWEDVQIQHGIGDLHIDLTKAANIKENNTI
                     VVRHILGKVQVILPVNYNINLHVAAFYGSTYVNEKSYKVENNNIHIEEMMKPDNYTVN
                     IYVSTFIGDVEVIYR"
     gene            6820..7863
                     /locus_tag="PPY92_001639"
     CDS             6820..7863
                     /locus_tag="PPY92_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDJ2625634.1"
                     /translation="MNHYIRTIGSMLILVYSMLAAFLFIDKVFVNIIYFQGMFYTQIF
                     GIPVFLFLNLIIILLCIIVGSVLAYKINQQNDWIKTQIERSMEGETVGINDQNIEIYS
                     ETLDLYHTLVPLNQELHKLRLKTQNLTNENYNINDVKVKKIIEDERQRLARELHDSVS
                     QQLFAASMMLSAIKETKLEPPLDQQIPILEKMVQDSQLEMRALLLHLRPLGLKDKSLG
                     EGIKDLVIDLQKKVPMKVVHEIQDFKVPKGIEDHLFRITQEAISNTLRHSNGTKVTVE
                     LFNKDDYLLLRIQDNGKGFNVDEKLEQSYGLKNMRERALEIGATFHIVSLPDSGTRIE
                     VKAPLNKEDSYDD"
     gene            7853..8482
                     /gene="vraR"
                     /locus_tag="PPY92_001640"
     CDS             7853..8482
                     /gene="vraR"
                     /locus_tag="PPY92_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator VraR"
                     /protein_id="HDJ2625635.1"
                     /translation="MTIKVLFVDDHEMVRIGISSYLSTQSDIEVVGEGASGKEAIAKA
                     HELKPDLILMDLLMEDMDGVEATTQIKKDLPQIKVLMLTSFIEDKEVYRALDAGVDSY
                     ILKTTSAKDIADAVRKTSRGESVFEPEVLVKMRNRMKKRAELYEMLTEREMEILLLIA
                     KGYSNQEIASASHITIKTVKTHVSNILSKLEVQDRTQAVIYAFQHNLIQ"
     gene            complement(8595..9812)
                     /locus_tag="PPY92_001641"
     CDS             complement(8595..9812)
                     /locus_tag="PPY92_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDJ2625636.1"
                     /translation="MSKKDHSSSKYLNSVKEAQEESKKKNKSNPKIDVDRTYIEPQQF
                     QSKKPKKDDQVFFLSRLNKPAKYKKDSNFLSYLIYRIGKDDASGLAAQMTYHFVLAMF
                     PMLLFLLTLLPFFNIKQSQITNMLSNAPAETSTLIKSVIGDITQNSSGGLLSIGLILA
                     IWSASNGMTAIMNSFNVAYDVEDSRNGIVLKLLSVVFTVVMGVVFVVALALPTLGSVI
                     SHFLFGPLGFDEQVKWIFNLIRIVLPIIIIFIIFIVLYSVAPNVKTKLKSVLPGAVFT
                     SIIWLAGSFGFGWYISNFGNYSKTYGSIAGIIILLLWLYITSFIIIVGAEINAIIHQR
                     SVIKGKTPEEAALEHDDNNKNHYNENTRYEYDEDSNATHQRTYHVDEHPSDTYPEDDK
                     NIKDKIVDKLKKD"
BASE COUNT         3553 a         1406 c         1778 g         3118 t
ORIGIN      
        1 aaatgagaat gattataatt tagaaacact gtatcaattt gtgattgaga aacttttttg
       61 aagttgcctt gtcatgttat aatgatgcga gtggaaattt gaggaggaaa cacatgagac
      121 agtggacggc aatccatcta gcgaaattgg cgcgtaaagc aagtagagca gtaggtaaaa
      181 gaggaacaga tttacctgga caaatcgcta gaaaagtgga tacagatgta ttaagaaaat
      241 tagcagagca agttgatgat attgtattta tcagtggaac aaatggtaaa acaacgactt
      301 caaacttaat tggacatact ttaaaagcaa ataatattca aattatacac aataatgaag
      361 gtgctaatat ggctgcaggt ataacttctg cattcatcat gcaatcaaca cctaagacta
      421 aaattgcggt aatcgaaatt gatgaaggtt cgattccacg tgtgttaaaa gaagttacac
      481 cttcaatgat ggtatttact aatttcttta gagatcaaat ggatcgcttc ggtgaaattg
      541 atattatggt taataacatt gcagagacaa ttagtaataa aggcatcaaa ttattgctaa
      601 atgctgatga tccatttgtg agtcgtttga aaatcgcaag tgatacgatt gtgtactatg
      661 gtatgaaagc acatgcccat gaatttgaac aaagtacgat gaatgaaagt agatattgtc
      721 caaactgtgg tcgcttattg caatacgatt atattcatta taatcaaatt ggtcattatc
      781 actgtcagtg tggtttcaaa cgagagcaag caaaatatga aatatcaagt tttgatgtgg
      841 caccgttttt atatttaaat atcaatgatg aaaaatatga tatgaaaatt gcaggtgact
      901 ttaacgctta taacgcgtta gcagcatata ctgttttaag agagctaggg ttaaatgaac
      961 aaacaattaa aaatggcttt gaaacgtata catcagacaa tggtcgtatg cagtacttta
     1021 aaaaagaacg aaaagaagcg atgatcaatt tagctaaaaa tcctgcagga atgaatgcaa
     1081 gtttatcagt tggtgaacaa ttagaaggcg aaaaagtgta tgttatttcg ctaaatgata
     1141 acgctgcaga tggtcgagat acttcatgga tttatgatgc agattttgaa aaattatcta
     1201 agcaacaaat tgaagctatc atcgtgacag gtacacgagc agaagaactt caattgcgat
     1261 tgaagttagc agaggttgaa gtaccaatta tagttgagcg tgatatttat aaagcaacgg
     1321 caaagactat ggattataaa ggtttcacag ttgcaatacc aaactataca tcattagcgc
     1381 ctatgcttga acaattaaac cgttcgtttg aaggaggtca atcataatat gcatgaattg
     1441 actatttatc attttatgtc agataaattg aatttataca gtgatatagg aaatattatt
     1501 gctttaagac aacgtgctaa aaaacgaaat attaaagtta atgtcgtaga aatcaatgaa
     1561 acagaaggta ttacctttga tgaatgtgat attttcttta tcggtggtgg aagtgataga
     1621 gaacaagcat tagcaacaaa agaattaagt aaaattaaga caccacttaa agaagcgatt
     1681 gaagatggta tgccgggatt aacaatttgt ggaggctatc aatttttagg aaaaaaatat
     1741 atcacgcctg atggtacaga attagaaggg ttaggtattt tagattttta tactgaatca
     1801 aagacaaacc gattaacagg agatattgtt atcgaaagtg atacttttgg aactattgta
     1861 ggttttgaaa atcacggtgg tagaacatat catgatttcg gtacacttgg tcatgttact
     1921 tttggttatg gtaataatga tgaagataaa aaagaaggca ttcattataa aaatttatta
     1981 ggtacttatt tacacggacc aattttacct aaaaattacg aaatcactga ttatctgtta
     2041 gaaaaagctt gtgaacgtaa ggggattccg tttgagccta aagaaataga taatgaagcg
     2101 gaaatacaag cgaaacaagt attaatagac agagcaaata gacagaagaa atctcgttaa
     2161 ctctgaacat cgcatcaatg gatttaatat tgataaacga tgaagtttag taataaatca
     2221 tatatgtata aacacacaca ttattttgga tggaaacaac caaattgatg tgtgtttttt
     2281 tgttctagtg aataattatt atacaatgag tatctatcct agaattatca atagtaatgg
     2341 tgattatgca acatgaaaaa atgaatgatg aaaggaattt gacgatgaag cccactaaag
     2401 tgattttaag agatgcatct tatttacata gcaaaacatc gataacattt attttaaaag
     2461 atgtagttat cgaagaagat aataaaattt attatttcga cactagtgca actttcgaag
     2521 atcaagaagt taaatttgaa tttgcacttt ttgatagtga catggacaat ttgaaacata
     2581 tggattacga caatcctgtt actgaaatat attttataga acctgattta cattttacaa
     2641 ttattgattt taatcaagaa ctgctttgta tttatattga ttttgattct ggtttaaggc
     2701 attcaaacat ggcaacagaa tctggtattt cattaaggat aaatattgct aaatcagatt
     2761 ttactaaatt tattaatgaa ttagcctctt tatattaatg atttaaatct gatacataat
     2821 tacaatcaaa aaagacagcc acatccctcc gtagtttagg cgtgtggcta tatttgagtc
     2881 tgaatattta tgcttgtaat tttaaaaagg gacatgctat atacgataaa aagaggcggg
     2941 gacataaatc aatgttctat gctctacgaa gttatattgg cagtagttga ctgaacgaaa
     3001 atgcgcttgt aacaagcttt tttcaattct agtcaggggc cccaacaaag agaaattgga
     3061 ttcccaattt ctacagacaa tgcaagttgg ggtgtgggtc ccaacacaga gaatttcgaa
     3121 aagaaattct acaggcaatg cgagttgggg tgggacgacg aaataaattt tacgaaaata
     3181 tcatttctgt cccactccca tggtgccaat tagcataagg tacttaaatt aagcatatct
     3241 gctgtctagc agtcgataaa tcattagaac ttcgtatagt atatgacttt taatttgatt
     3301 ttcaccacta atttcaagtg cttttatagt cgaacgtaaa gtttctacag aatcatcttc
     3361 tctcttaaaa gtaccatcat aaaatatatc tttgatgcta ctactaattt ttagcaatgc
     3421 cattttttcg tcacctgaaa agttaacacg agtattttta ggcaagtaaa tgatatttga
     3481 taaatgagtg ataaacaaac gattcgtata tgcacgttta gttaattgat tgagtaattt
     3541 ccaatcacat tcttttttct tatgatagct taattcatca cgttgataac ttattaacgt
     3601 ttcaacttga ttgtttaaat tgaaaatatt tttatatgct ttttcgcttt tatcagattg
     3661 cagtcttgat aagataagtt cttggcagcg attgtaaaat aatttataca tcaaggcatc
     3721 tgtcttactt aatttttctt cgacctgacc ataatactta ggtggaaaca ccatgaagtt
     3781 aattaaacct gatgtcacga gtccaataat tgctgtcaat gttcgagaca aaaagttgaa
     3841 tatgtaggca tcatgaatac ctggaatcat agctaatgat gttagtacag cgacattcgt
     3901 accaacttgc aatttgagtt ttgtacagaa taaaatcgtg aacgttgcac tcaatgcata
     3961 tgtaaaaggt gattgatcgc cgaataaata tgtaaataat actgcaaagc ctgcaccaat
     4021 taccgtagca ggtaatctac gataaccttt aataagtgat gccttggcag ttggttcaat
     4081 tgtgactaca gctgttaaaa tggcatagat gggtgttaaa tctagtgcca tacaaaagac
     4141 agctgttaaa aaaatggcaa taccagtttt aattgttctg gcaccaatta aatgtttata
     4201 ccattgatcg ttcatttttt aacctctaat catcgtaaaa tcttagcgag cgctttataa
     4261 taatagtatc gtacattgga aaagttcatg tatgtaaaat atttgaaata atcatactta
     4321 agcattactt tgattttcat atacattaat caaagttctg ggctccacaa tgttatattg
     4381 ttagtagttg actgaaataa aatgcacttg taacaagcat ttttcaatta tagtccgggg
     4441 ccccaacaca gaagctgacg aaaagtcagc ttacaataat gtgcaagttg gggtgtgggc
     4501 cccaacacag aagctgacga aaagtcagct tacgataatg tgcaggttgg cggggcccca
     4561 acatagagaa attggatcta caatttctac aggcaatgca agttggggta caacgataaa
     4621 gaaatatttt ttctttatca cactatgtct cactcacttt ccaaaatact aaagtaacat
     4681 ctttagtata tcaaagaatt tttgctataa taagttataa ttatataaaa aaggaacggg
     4741 ataaaatgat tgtaaaaaca gaagaagaat tacaagcgtt aaaagaaatt ggatacatat
     4801 gcgctaaagt gcgcaataca atgcaagctg caaccaaacc aggtatcact acgaaagagc
     4861 ttgataatat tgcgaaagag ttatttgaag aatacggtgc tatttctgcg ccaattcatg
     4921 atgaaaattt tcctggtcaa acgtgtatta gtgtcaatga agaggtggca catgggattc
     4981 caagtaagcg tgtcattcgt gaaggagatt tagtaaatat tgatgtatcg gctttgaaga
     5041 atggctatta tgcagataca ggcatttcat ttgtcgttgg agaatcagat gatccaatga
     5101 aacaaaaagt atgtgacgta gcaacgatgg catttgagaa tgcaattgca aaagtaaaac
     5161 cgggtactaa gttaagtaac attggtaaag cggtgcataa tacagctaga caaaatgatt
     5221 tgaaagtcat taaaaactta acaggtcatg gtgttggttt atcattacat gaagcaccag
     5281 cacatgtact taattacttt gatccaaaag acaaaacatt attaactgaa ggtatggtat
     5341 tagctattga accgtttatc tcatcaaatg catcatttgt tacagaaggt aaaaatgaat
     5401 gggcttttga aacgagcgat aaaagttttg ttgctcaaat tgagcatacg gttatcgtga
     5461 ctaaggatgg tccgatttta acgacaaaaa ttgaagaaga atagttcaac atatactaag
     5521 actaaagtat gaacatcatt tagttccgga gcctattcat attggtttcg gaactgtttt
     5581 ataataatta agaacacaat caatgcgtca tttcaaaaat atgttgtaat aaagtagttt
     5641 ttaagcaaac atatcatcga catcaacgaa gatacatagc gcatttggta ttttaaaact
     5701 tattataaaa ggtgatagtt atgaactatg ttgaacgtta tattgaacag tttttgagag
     5761 caacagtaag aaataatatc aagcactacc ttttaatgct agatgaaaaa atgaaaaatt
     5821 tagatgatta tatgcgttat ttaattacta aaaaagaaca acttagcaag ttaattgaca
     5881 gtctaatgct aacattagaa aataaatata ttgatattgc tgaagcattt caaattcaat
     5941 gtgcaagaga aatcaataat caagaaattg aaaatattaa atcagagttg aataaagttg
     6001 aagcatatta tgcacaaatt gaaactcaaa ttcaacaaac ttcaactgaa aaaatagcaa
     6061 cagaaaaaac atcgtatcta ataaattata tgaacgctgt ggcatagaaa ggcggcgaaa
     6121 catgacacac aaatatatat caacgcaaat gttgatcatt tttactgcat taatgattat
     6181 tgccaatttt tactacatat tttttgaaaa aattggcttt ttactcgttc tattattggg
     6241 atgtgtatta gtttatgtag gatatcttta ttttcataaa atacgtggcc ttttagcgtt
     6301 ttggataggc gcgctattaa ttgcattcac attattgtct aataagtata caatcatcat
     6361 cttgttcgtc tttttattac tacttattgt gcgttattta atacataaat ttaaaccaaa
     6421 aaaagtagtt gcgacggatg aggttatgac ttcaccatct tttattaaac aaaagtggtt
     6481 tggtgagcaa cgtacaccag tttatgtata taagtgggaa gatgtccaaa ttcaacatgg
     6541 aattggcgac ctacatattg acttaacaaa agctgcaaat attaaggaaa ataataccat
     6601 tgttgttaga cacattttag gtaaagtgca ggttatattg ccggttaatt acaatattaa
     6661 tttacatgta gctgcttttt atggaagtac ttacgtgaat gaaaaatcat ataaagttga
     6721 aaataacaat attcatattg aagaaatgat gaaaccggat aactatacag ttaatatcta
     6781 cgtatcaacg tttatcggag acgtagaggt gatttatcga tgaaccacta cattagaaca
     6841 attggttcaa tgctcatctt agtatatagc atgctagctg catttctatt catcgataaa
     6901 gtttttgtaa atatcatcta ttttcaaggt atgttttata cacaaatatt cggaatacca
     6961 gtctttttat ttttaaatct catcatcata ttattgtgta ttattgttgg ttcggtactc
     7021 gcatacaaaa tcaatcagca aaatgattgg attaagacgc aaattgagcg ttcaatggaa
     7081 ggcgaaacag ttggcattaa tgatcaaaat atagaaatat atagtgaaac gttagattta
     7141 taccatacac tcgtaccttt aaatcaagaa ttgcataagt tgcgacttaa aactcaaaac
     7201 ttaaccaatg aaaattataa tattaatgat gtgaaagtta aaaagattat tgaagatgaa
     7261 cgtcaaagac tagctcgaga acttcacgat tctgttagtc agcaactttt tgcggcaagt
     7321 atgatgcttt ctgctatcaa agaaacgaag ttagaaccac cattagacca acaaattccg
     7381 attttagaga aaatggttca agattcgcag ttagaaatgc gtgctttgct gttacattta
     7441 agaccgcttg gtttaaaaga caaatcttta ggtgagggta ttaaagattt agttattgat
     7501 ttacaaaaaa aagtgccaat gaaagttgtg catgaaatac aagattttaa agtgcctaaa
     7561 ggtattgaag atcatttgtt cagaattaca caggaagcaa tttcgaatac attgcgtcat
     7621 tcaaacggta caaaagtgac agtagaattg tttaataaag acgattattt attgttgaga
     7681 attcaagata atggtaaagg ttttaatgtt gatgaaaaat tagaacaaag ttatggactt
     7741 aaaaatatgc gtgaaagagc tttggaaatt ggtgcaacgt tccatattgt atcattgcca
     7801 gattcaggta cacgtatcga ggtgaaagca cctttaaata aggaggattc gtatgacgat
     7861 taaagtattg tttgtggatg atcatgaaat ggtacgtata ggaatttcaa gttatctatc
     7921 aacgcaaagt gatattgaag tagttggtga aggcgcttct ggtaaagaag caattgccaa
     7981 agcccatgag ttgaagccag atttaatttt aatggattta cttatggaag acatggatgg
     8041 tgtagaagcg acgactcaga ttaaaaaaga tttaccgcaa attaaagtat taatgttaac
     8101 tagttttatt gaagataaag aggtatatcg tgcattagat gcaggtgtcg atagttacat
     8161 tttaaaaaca acaagtgcaa aagatatcgc cgatgcagtt cgtaaaactt ctagaggaga
     8221 atctgttttt gaaccggaag ttttagtgaa aatgcgtaac cgtatgaaaa agcgcgcaga
     8281 gttatatgaa atgcttacag aacgagaaat ggaaatatta ttattgattg cgaaaggtta
     8341 ctcaaatcaa gaaattgcta gtgcatcgca tattactatt aaaacggtta agacacatgt
     8401 gagtaacatt ttaagtaagt tagaagtgca agatagaaca caagctgtca tctatgcatt
     8461 ccaacataat ttaattcaat agttcgtatc gaattaagaa aagttactta cgccaatcac
     8521 aatataacat caaatagaca ccaaaaaaca gaggtcttcg caatcattag cgaaaacctc
     8581 tgttttgttg gtgtttagtc ttttttaagc ttatcaacga ttttgtcttt tatattttta
     8641 tcatcttctg gataggtatc actaggatgt tcatcaacat gatacgtacg ttgatgtgtt
     8701 gcattactat cttcatcata ttcgtatcta gtgttctcat tataatgatt tttattattg
     8761 tcatcatgtt ctaatgctgc ttcttcaggt gttttacctt taattacact acgctgatga
     8821 atgattgcat tgatttcagc accgacaatt ataataaaac ttgtgatata taaccatagt
     8881 aataaaatga tgatacccgc gatactgcca tatgttttag aatagttacc aaaatttgaa
     8941 atataccaac caaaaccaaa tgaaccagct aaccaaataa ttgaagtaaa tactgcacct
     9001 ggtaatactg acttaagctt cgttttaacg ttaggtgcaa ccgaatataa cacgataaat
     9061 atgataaata taataatgat tggtaacaca attctaataa ggttaaaaat ccatttcact
     9121 tgttcgtcaa atccaagtgg accgaatagg aaatgactaa ttacagaacc aagcgttggt
     9181 aatgctagag caactacaaa cacaacgccc ataactacag tgaagacaac acttagtagt
     9241 tttaaaacga ttccattacg gctatcttct acatcgtaag caacattgaa agaattcata
     9301 attgcagtca ttccatttga agctgaccaa attgctaaaa tcaaaccgat agataataag
     9361 ccaccactgg agttttgagt tatatcacca attacactct taattagagt agatgtttca
     9421 gcgggtgcat tgcttaacat attagtaatt tgactctgct taatattgaa aaatggtaat
     9481 aatgttaata ggaaaagcaa catagggaac atagcaagta cgaaatggta agtcatttgc
     9541 gctgctagtc ctgaggcatc atcttttcct atgcgataga tgagatatga taagaaatta
     9601 gagtctttct tatattttgc aggtttattt aatcttgata agaagaaaac ctgatcatct
     9661 tttttaggtt tcttagattg gaattgttga ggttcaatat atgtacgatc aacatcaatt
     9721 ttgggattac ttttattttt cttttttgac tcctcttgcg cttccttaac agaattaagg
     9781 tattttgaag aagagtgatc ctttttcgac atgtgcactc cttaaccttt ctctatgtaa
     9841 tgttttataa agtaa
//
LOCUS       DANBWH010000098         9767 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.101, whole genome shotgun sequence.
ACCESSION   DANBWH010000098 DANBWH010000000
VERSION     DANBWH010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9767
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.101"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(56..130)
                     /locus_tag="PPY92_001642"
     tRNA            complement(56..130)
                     /locus_tag="PPY92_001642"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(95..97),aa:Glu,seq:ttc)
     gene            complement(559..1842)
                     /locus_tag="PPY92_001643"
     CDS             complement(559..1842)
                     /locus_tag="PPY92_001643"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDJ2625637.1"
                     /translation="MSSIVVVGTQWGDEGKGKITDFLAEQSDVIARFSGGNNAGHTIQ
                     FGGETYKLHLVPSGIFYKDKLAVIGNGVVVDPVALLKELDGLNERGIPTSNLRISNRA
                     QVILPYHLAQDEYEERLRGDNKIGTTKKGIGPAYVDKVQRIGIRMADLLEKETFERLL
                     KSNIEYKQAYFKGMFNETCPSFDDIFEEYYAAGQRLKEFVTDTSKILDDAFVADEKVL
                     FEGAQGVMLDIDHGTYPFVTSSNPIAGNVTVGTGVGPTFVSKVIGVCKAYTSRVGDGP
                     FPTELFDEDGHHIREVGREYGTTTGRPRRVGWFDSVVLRHSRRVSGITDLSINSIDVL
                     TGLDTVKICTAYELDGKEITEYPANLDQLKRCKPIFEELPGWTEDVTSVRTLEELPEN
                     ARKYLERISELCNVQISIFSVGPDREQTNLLKELW"
     gene            complement(2120..3520)
                     /gene="dnaB"
                     /locus_tag="PPY92_001644"
     CDS             complement(2120..3520)
                     /gene="dnaB"
                     /locus_tag="PPY92_001644"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDJ2625638.1"
                     /translation="MDRMYEQNQMPHNNEAEQSVLGSIIIDPELINTTQEVLLPESFY
                     RGAHQHIFRAMMHLNEDNKEIDVVTLMDQLSTEGTLNEAGGPQYLAELSTNVPTTRNV
                     QYYTDIVSKHALKRRLIQTADSIANDGYNDELELDAILSDAERRILELSSSRESDGFK
                     DIRDVLGQVYETAEELDQNSGQTPGIPTGYRDLDQMTAGFNRNDLIILAARPSVGKTA
                     FALNIAQKVATHEDMYTVGIFSLEMGADQLATRMICSSGNVDSNRLRTGTMTEEDWSR
                     FTIAVGKLSRTKIFIDDTPGIRINDLRSKCRRLKQEHGLDMIVIDYLQLIQGSGSRAS
                     DNRQQEVSEISRTLKALARELECPVIALSQLSRGVEQRQDKRPMMSDIRESGSIEQDA
                     DIVAFLYRDDYYNRGGDEDDDDDGGFEPQTNDENGEIEIIIAKQRNGPTGTVKLHFMK
                     QYNKFTDIDYAHADMM"
     gene            complement(3552..3998)
                     /gene="rplI"
                     /locus_tag="PPY92_001645"
     CDS             complement(3552..3998)
                     /gene="rplI"
                     /locus_tag="PPY92_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDJ2625639.1"
                     /translation="MKVIFTQDVKGKGKKGEVKEVPVGYANNFLLKKNYAVEATPGNL
                     KQLELQKKRAKQERQQEIEDAKALKETLSNIEVEVSAKTGEGGKLFGSVSTKQIAETL
                     KAQHDIKIDKRKMDLPNGIHSLGYTNVPVKLDKEVEGTIRVHTVEQ"
     gene            complement(3995..5962)
                     /gene="gdpP"
                     /locus_tag="PPY92_001646"
     CDS             complement(3995..5962)
                     /gene="gdpP"
                     /locus_tag="PPY92_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP phosphodiesterase GdpP"
                     /protein_id="HDJ2625640.1"
                     /translation="MNRQSTKKALLIPFVIMIITAIVLMGVWFIFNSLIALIASIVLV
                     VMIIVSIILFRQALMKMDSYVDGLSAQISTTNNKAIKHLPIGIIVLDENDHIEWVNQF
                     MTNHMEANVISESVNEVFPNILKQLDRVKSVEIEYNQYHFQVRYSENDHCLYFFDITE
                     QVQTNELYENSKPIIATLFLDNYDEITQNMNDTQRSEINSMVTRVISRWATEYNIFFK
                     RYSSDQFVAYLNQKILADLEESKFDILSQLREKSVGYRAQLTLSIGVGEGTENLIDLG
                     ELSQSGLDLALGRGGDQVAIKSINGNVRFYGGKTDPMEKRTRVRARVISHALKDILAE
                     GDKVIIMGHKRPDLDAIGAAIGVSRFAMMNNLEAYIVLNETDIDPTLRRVMNEIDKKS
                     ELRERFITSDDAWDMMTSKTTVVIVDTHKPELVLDENVLNKANRKVVIDHHRRGESFI
                     SNPLLIYMEPYASSTAELVTELLEYQPTEQRLTRLESTVMYAGIIVDTRNFTLRTGSR
                     TFDAASYLRAHGADTILTQHFLKDDVDTYINRSELIRTVKVEDNGIAIAHGSDDKIYH
                     PVTVAQAADELLSLEGIEASYVVARREDNLIGISARSLGSVNVQLTMEALGGGGHLTN
                     AATQLKGVTVEEAIAQLQQAITEQLSRSEDA"
     gene            complement(5977..6915)
                     /locus_tag="PPY92_001647"
     CDS             complement(5977..6915)
                     /locus_tag="PPY92_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HDJ2625641.1"
                     /translation="MGVAYVFSKIQPKATIIATITLVFVALALYLVPGLGLIFALFAT
                     IPGIVLWNKSIQSFGVSALITVIITTVLGNTFVLSAIILVLIASLIIGQLLKERTSKE
                     RMLYVTTVAMSLISLIAFMLLQTFGRIPPSASIVKPFKQTLHEAITMSGADANMTQIL
                     EEGFRQATVQLPGFIIIITFLIVLINLIVTFPVLRKFKIATPVFKPLFAWQMSGILLW
                     IYIIVIICLLFTGQPSVFQSILLNFQLVLSLVMYIQGLSVIHFFGKAKGLPNAVTILL
                     LIIGTILTPTTHIVGLLGVIDLSLNLKRIMKNNSKK"
     gene            complement(7208..8176)
                     /locus_tag="PPY92_001648"
     CDS             complement(7208..8176)
                     /locus_tag="PPY92_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDJ2625642.1"
                     /translation="MTNYTVDTLNLGKFITESGEVIDNLRLRYEHVGYHGQPLVVVCH
                     ALTGNHLTYGTDDYPGWWREIIDGGYIPIHDYQFLTFDVIGSPFGSSSPLNDPHFPKK
                     LTLRDIVRANERGIQALGYDKINILIGGSLGGMQAMELLYNQQFEVDKAIILAATSRT
                     SSYSRAFNEIARQAIHLGGKEGLSIARQLGFLTYRSSKSYDERFTPDEVVAYQQHQGN
                     KFKEHFDLNCYLTLLDVLDSHNIDRGRTDVTHIFKNLETKVLTMGFIDDLLYPDDQVR
                     ALGERFKYHRHFFVPDNVGHDGFLLNFSTWAPNLYHFLNLKHFKRK"
     regulatory      complement(8236..8334)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(8539..8868)
                     /locus_tag="PPY92_001649"
     CDS             complement(8539..8868)
                     /locus_tag="PPY92_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDJ2625643.1"
                     /translation="MITHMNMLILILLCGIVTLLIRIIPFIMISKVQLPDVVVRWLSF
                     IPITLFTALVIDSIIQQTPHGEGYTLNIPYIIALIPTVILSIITRSLTITIISGIVIM
                     ATLRFFF"
     gene            complement(8865..9560)
                     /locus_tag="PPY92_001650"
     CDS             complement(8865..9560)
                     /locus_tag="PPY92_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDJ2625644.1"
                     /translation="MATHLSFRQGVQECIPTLLGYAGVGISFGIVASSQNFSILEIIL
                     LCLVIYAGAAQFIMCALFIAGTPISAIVLTVFIVNSRMFLLSMSLAPNFKTYGFWNRV
                     GLGSLVTDETFGVAITPYLKGEAINDRWMHGLNITAYLFWAISCVAGALFGEYISNPQ
                     TLGLDFAITAMFIFLAIAQFESITKSRLRIYIVLIIAVIVMMLSLSMFMPSYLAILIA
                     ATISAALGVMMER"
BASE COUNT         3051 a         1917 c         1477 g         3322 t
ORIGIN      
        1 gatctcctgc gtgacaggca ggcgtgttaa ccgctacact acgagaccta aaaaatggtg
       61 actcctacgg gactcgaacc cgtgttaccg ccgtgaaagg gcggtgtctt aaccgcttga
      121 ccaaggagcc gttcttaagc acaagataga ttatagcaca gccaatcttg ccatgacaag
      181 atatttttta cataattttg ctattatttt ttgaagaatg ttataagtga aaaattgcat
      241 tttattggca gtctatttat tataataatt acgctatttt tgaaattttt ctattttagg
      301 tactttcatt tacacttacg tctactctat tttaatagct attttcgaca tcagacttat
      361 taaattcaag tatttcttct taattttgtt aaatatcatc taatctttta aattatgact
      421 agcaaatatc acctttacgt tagtaatttt tatctaaata atcgaatcga tacagaagca
      481 aacaaagcaa aagcctccta ccaataaaga tagaaggctc atgtatttat aatcattgcg
      541 tatgacttat ataaagttct accacaattc ttttaatagg tttgtttgtt ctctatctgg
      601 accaactgag aagatagaaa tttgtacatt acataattct gaaatacgct ctaaatattt
      661 acgtgcattt tcaggtaatt cttctaaagt acgcacactt gttacgtctt ctgtccaacc
      721 tggtaactct tcaaagattg gtttacaacg ttttaattga tctaagtttg ctgggtactc
      781 agtaatttct ttaccgtcta attcataagc tgtacagatt ttcactgtgt ctaggcctgt
      841 taaaacatcg attgagttaa tagataaatc tgtaatacca cttacacgac gagagtgacg
      901 taatacaact gagtcaaacc aacctacacg acgtggacgt cctgttgttg taccgtattc
      961 acgaccaact tctctaatat gatgtccatc ttcatcgaat aattcagtag ggaatggacc
     1021 atcaccaaca cgtgatgtat aagctttaca tacaccaatt accttcgaaa cgaatgtagg
     1081 acctacacct gtaccaacag taacgttacc tgcaattgga ttacttgatg taacgaatgg
     1141 atatgtacca tggtcgatat ctaacattac accttgcgca ccttcgaaaa gtaccttttc
     1201 atctgctaca aatgcatcgt ctaagatttt tgatgtgtct gttacaaatt cttttaaacg
     1261 ttgacctgca gcatagtatt cttcaaagat atcatcaaat gatggacatg tttcgttaaa
     1321 catacctttg aaatatgctt gtttatattc aatgtttgat tttaataatc tttcgaatgt
     1381 ttctttttca agtaaatctg ccatacgaat accgatacgt tgaactttgt ctacatatgc
     1441 tggaccgata ccttttttag ttgtaccaat cttattgtca ccgcgtaaac gttcttcata
     1501 ttcatcttgt gctaagtgat atggtaaaat cacttgcgca cgattagata tacgtaaatt
     1561 acttgtagga atgccacgtt catttaatcc gtctaattct ttcaatagtg caactggatc
     1621 aacaacgaca ccgttaccga ttaccgctaa tttgtctttg taaaagatac cagatggtac
     1681 taaatgtaat ttatatgttt ctccgccaaa ttgaatggta tggcctgcat tattaccacc
     1741 tgaaaaacgc gcgataacat ctgactgttc tgccaagaaa tccgttattt ttccttttcc
     1801 ttcgtctccc cattgtgtcc caactactac gattgatgac atgtgagcac ctccaagttt
     1861 ttctcattta accatttgat attctaccaa tatcgagacc ataatgcaaa taaaaatcga
     1921 acattgattt tcggcctttt taaaagaaaa atcattttaa caaatcgtct tcaacgtcct
     1981 aaaaaccctt taacagaggc gttgacagta ctaatttatc actttcaata cacaaactta
     2041 acatatatca acccgttcag aacaaaataa aaaccgtaca attttatcat cttaatgatt
     2101 attgtacgga aaaacttttt tacatcatat ctgcatgtgc ataatcgata tcggtaaatt
     2161 tattatattg tttcataaag tgtaacttaa ctgtgcctgt tggaccgtta cgttgcttag
     2221 caatgataat ttcaatttca ccgttttcat cattcgtttg tggctcgaaa ccaccatcat
     2281 cgtcatcatc ttcatcgccg ccacggttat agtaatcatc acggtataag aatgcaacga
     2341 tatcggcatc ttgctcaatc gaaccagatt cacgaatatc actcatcatt ggacgtttat
     2401 cttgtcgttg ttcaacacca cgagataact gacttaatgc gataactgga cattctaatt
     2461 cacgggctaa tgcttttaat gtacgagaga tttcagaaac ttcctgttgt ctgttatcgg
     2521 acgcacgtga accactacct tgaatcaact gtaagtagtc aatcacaatc atgtctaagc
     2581 catgttcttg ctttaatcga cgacatttag aacgtaaatc attaattcga atacccggtg
     2641 tatcatcaat aaaaatcttc gtacgtgata atttacctac cgctatagta aaacgactcc
     2701 aatcttcctc agtcatagta cccgttctta agcggtttga gtcaacatta ccagaactac
     2761 aaatcatacg tgtggctaac tgatcagcac ccatctctag tgagaaaata ccaactgtat
     2821 acatatcttc atgcgttgca actttttgtg caatattaag tgcgaacgca gtcttaccta
     2881 cagatggacg cgctgcaagg ataattaaat catttcggtt gaaccctgct gtcatttggt
     2941 ctaaatctcg atatcctgta ggtatacctg gtgtttgacc actattttga tcaagctctt
     3001 cagctgtttc atacacttgt cctaagacgt ctcgaatgtc tttaaagcca tcgctttcac
     3061 gagaagatga tagctctaaa attcgacgtt ctgcatcact taaaatcgca tctagttcaa
     3121 gttcatcatt atatccatca ttggcaatac tatctgcagt ttgaatcaat ctacgtttta
     3181 atgcatgctt agaaacgata tcagtataat actgaacatt tcgcgtcgtt ggtacatttg
     3241 tagataactc tgcaagatat tgcgggccac ccgcttcatt caacgtacct tccgtcgata
     3301 attgatccat caatgttaca acatcaattt ctttattatc ttcatttaag tgcatcattg
     3361 cacggaaaat atgttgatgg gcacccctat aaaacgactc aggaagcaaa acttcctgag
     3421 tagtattaat caattctgga tctataataa ttgaacctaa gacagactgt tcagcttcat
     3481 tgttatgtgg catttgattt tgctcataca ttctatccat gaatggttac acctcttatt
     3541 tcaatccaac tttattgttc aactgtgtgt acgcgaattg taccttcaac ttctttatct
     3601 aatttaacag gtacattcgt atatcctagg gaatgaattc catttggtaa atccatttta
     3661 cgtttatcaa ttttaatatc atgttgtgct tttagtgttt cggcaatttg ttttgtactt
     3721 actgatccaa acaatttacc accttcacca gtttttgctg atacttcaac ttcaatgttt
     3781 gataacgttt cttttaatgc tttagcatct tcaatttctt gttggcgttc ttgttttgca
     3841 cgttttttct gtaactctaa ttgtttaagg ttacctggtg ttgcttctac agcataattc
     3901 tttttcaata agaagttatt tgcataacct actggtactt ctttaacttc acctttttta
     3961 cctttacctt taacatcttg tgtaaaaatt actttcatgc atcttcactc ctacttaatt
     4021 gttctgtaat tgcttgttgt aattgtgcta tcgcctcttc gactgtcaca cctttaagtt
     4081 gtgttgcagc attggttaaa tgtccaccgc caccaagtgc ttccattgtt aactgaacat
     4141 ttactgaacc gagtgaacgc gcagatatac caatcagatt atcttcacgt ctcgcaacaa
     4201 catatgatgc ttcaatacct tctaaactta acagttcatc tgctgcttgt gcaactgtta
     4261 ctggatgata aattttatcg tctgaaccat gcgcaatggc tatgccatta tcttcaactt
     4321 ttacagttcg aattaattca gatcgattaa tgtaagtatc cacatcatct tttaagaaat
     4381 gttgcgttaa aatcgtatct gcaccatgtg cacgtaaata actcgctgca tcgaatgttc
     4441 ttgatcctgt tcgtaatgta aagtttcttg tatctacaat aatacctgca tacatcactg
     4501 ttgattcaag acgtgttaaa cgttgttctg ttggttgata ttccagtaac tctgttacca
     4561 attcagctgt tgaacttgcg tatggttcca tatatatcaa caatggatta gagatgaagc
     4621 tttcaccacg tctatgatga tcgataacaa ctttacggtt tgctttattt aagacatttt
     4681 catctaaaac cagttccggt ttatgcgtat caacaatcac tacggttgtc ttagatgtca
     4741 tcatatccca agcatcatct gatgttataa atcgctctct taactctgac tttttatcaa
     4801 tttcgttcat cacgcgtcgt aatgttggat caatgtcagt ctcatttaat acgatgtatg
     4861 cttctaaatt attcatcatt gcaaatctag acacaccgat tgctgcacca attgcatcta
     4921 agtcaggacg tttatgtccc atgataatga ctttgtcacc ctctgcaagg atatctttta
     4981 acgcatgtga gatcactcgt gctcttacac gagtacgttt ctccatcggg tcagtcttac
     5041 cgccatagaa acgcacatta ccattaatac ttttaattgc aacttggtcg ccaccgcgcc
     5101 ctaatgctaa gtctaggcct gattgtgata attcacctaa atcgattaaa ttttcagtac
     5161 cttcaccaac accaatactt aatgttaatt gggcacgata accaacactt ttttcacgta
     5221 attgactcaa gatatcaaat ttagattctt ctaagtcagc taatattttt tgatttaaat
     5281 aggctacgaa ttgatcggaa ctataccttt taaagaatat attatactca gttgcccatc
     5341 gactaataac acgcgttacc attgaattga tttccgaacg ctgcgtatca ttcatatttt
     5401 gagtaatctc atcgtagtta tctaaaaata atgtcgcaat gattggttta gaattttcat
     5461 atagttcatt tgtttgtact tgttcagtta tatcaaagaa atagaggcaa tgatcattct
     5521 cagaataacg tacttggaaa tgatactgat tatattctat ttcaacggat ttcactctat
     5581 ctaattgctt taaaatgttt ggaaatactt catttacaga ttcagaaatg acatttgctt
     5641 ccatatgatt tgtcataaat tggttaaccc attcgatgtg atcattttca tctaaaacaa
     5701 tgataccaat tggtaaatgt ttgattgctt tattatttgt tgttgaaatt tgagcactca
     5761 aaccatctac ataactatcc attttcatta aagcttgtct gaataaaatg atgctaacaa
     5821 taatcatcac gacaagaacg atagatgcaa ttaatgctat aagactatta aagataaacc
     5881 atacacccat taaaacaatt gctgtgatga tcatgatgac aaatggtatt agtaaagctt
     5941 tcttagtgga ctgccgattc attattccac ctctattcac tttttagaat tatttttcat
     6001 gattcgcttc aaattcaaac ttaaatcgat aacaccaagt agtcctacaa tatgtgtcgt
     6061 aggtgtcagt attgtaccga taatcaatag taaaatcgtt actgcattcg gcaaaccttt
     6121 cgctttacca aagaaatgaa taacacttaa accttgaata tacattacta atgataacac
     6181 aagttggaag tttaaaagaa tgctctggaa cacactcggt tgacctgtaa ataataaaca
     6241 tatgataaca ataatgtata tccataataa aataccgctc atttgccacg cgaaaagtgg
     6301 cttaaataca ggtgtagcga ttttaaattt tcgtaaaacc ggaaatgtaa cgattaagtt
     6361 aattaagacg attaaaaatg taatgataat gatgaaacct ggtaattgaa cggtcgcttg
     6421 tctaaaccct tcttctaata tttgggtcat attcgcatcg gcaccgctca tcgtaatcgc
     6481 ttcatgtaat gtttgcttga aaggttttac tatgctcgct gatggtggaa tccttccgaa
     6541 tgtttgtagt aacataaaag cgattaatga aattaagctc atcgctactg ttgttacgta
     6601 taacattctt tctttagacg ttctttcttt gagcaattga ccaataatta aacttgcaat
     6661 taagactaat atgatggcac ttaaaacgaa agtattacct aaaacagttg ttataattac
     6721 tgtaataagt gcactaaccc cgaaagattg tattgattta ttccataaaa cgatacctgg
     6781 tatggttgca aataatgcaa atattagtcc caaaccaggc actagatata aagctaaagc
     6841 gacaaatacc aacgtaatcg ttgcaattat tgttgcttta ggttgtattt ttgaaaacac
     6901 ataagccact cccatatttt taactatagc tattatttta acctctttaa tggaaattaa
     6961 caatttatag attgtatgct tctatttcat ttaattgaat aataactttc atgttttata
     7021 agtaattaac atactcattt gaatcgcttt tgtgtgcttt cattttcaac atgattattt
     7081 aatcccacta catagcaatc aagcttgatt tagatttaca attcatttcc actctcatgt
     7141 actctagatg tctttgaata tgataactgt gatttagtag cttcattctt taaaaatata
     7201 tacattatta cttacgctta aaatgcttta aatttaagaa atgatataag ttaggtgccc
     7261 aggtactaaa gtttagtagg aatccatcat gtccaacatt atcaggcacg aagaaatgac
     7321 gatgatattt aaaacgttca cctaatgcac gaacttgatc gtccggatat agcaaatcat
     7381 ctatgaaccc catcgttaag actttcgttt ctaaattttt aaaaatatgc gttacgtctg
     7441 tgcgacctcg gtcaatgttg tgactatcca atacatctag cagtgtcaga taacaattca
     7501 aatcaaaatg ttctttaaat ttattacctt gatgttgttg gtatgcgact acttcatccg
     7561 gcgtgaaacg ttcatcataa ctttttgatg atcgatatgt caaaaaacct aattggcgtg
     7621 caatacttag accttcctta ccaccaagat gaatggcttg ccttgcaatt tcattgaaag
     7681 ctctactata agatgatgtt cgacttgttg cagcaaggat aatggcttta tctacttcaa
     7741 actgttgatt gtaaagtagt tccattgctt gcatacctcc aagacttccc cctattaaaa
     7801 tattaatctt atcataacca agggcttgta tacctcgttc attcgctctg actatatctc
     7861 ttaatgttaa ttttttagga aaatgagggt cgtttaaagg tgaacttgaa ccgaaaggac
     7921 taccaataac atcaaatgtt aaaaattgat aatcgtgaat gggtatatat cccccatcaa
     7981 taatttctcg ccaccaaccc ggataatcat ctgttccata tgttaaatga ttgccagtta
     8041 atgcatgaca aactacaact aatggttgtc catgataacc aacatgctca tatctcaaac
     8101 gcaagttatc tatgacttcc ccagattctg taataaattt ccctagattt aaagtatcta
     8161 ctgtgtaatt tgtcattgtt ctttcctcct taaacaaaaa aacttctcac cctattgaaa
     8221 agtaagaagt ctttatactt atcattcgag taactcgttg gttttagcac cgtgctataa
     8281 agtcggttgc tgaagtatca cagggccaag tccctcaact tctcacaata agaaatatga
     8341 aattgttatg tgttagttga gattcagtga tgaattactt ttatcattta aaatattgtt
     8401 atcattgtca tacgttagcc aaatcgcttt cgtgtatacg attcccagtc ataccataga
     8461 cgatttgtat atcagaattt tctgattact aacagtttac ctaagtttaa atatctgttc
     8521 aatgattttc agttattttt aaaagaaaaa tcgtaatgtt gccatgataa caatcccact
     8581 aataattgta atagttaaac tacgcgtgat tatagataaa ataaccgtcg gaatgagcgc
     8641 gataatgtaa gggatgttta atgtataccc ctcaccatga ggcgtctgtt gaataatgct
     8701 gtcaatgaca agtgccgtaa atagtgtgat tgggataaat gatagccatc gaaccacaac
     8761 atcaggcaat tgcacttttg aaatcatgat aaaaggtata attcgaatta atagcgttac
     8821 gataccacac aataaaataa gtattaacat gttcatatga gttatcatcg ttccatcatc
     8881 actcctaacg ctgctgaaat tgtggctgca attaatattg ctagatatga aggcataaac
     8941 atacttagcg ataacatcat tactatgacg gcaataatga gtactatgta aattcttaat
     9001 cgcgatttag taattgattc aaattgcgca atggccaaaa agataaacat agccgtgata
     9061 gcaaaatcta accctagcgt ttgcggattt gagatatatt cgccaaataa agccccagct
     9121 acacatgaaa ttgcccaaaa taaatatgct gtgatattaa gaccatgcat ccaacgatca
     9181 ttgatagctt ctccttttaa ataaggtgta atggcgacgc caaacgtttc gtcagttact
     9241 aatgaaccta atccaacacg gttccaaaac ccatatgtct tgaagtttgg tgcaagcgac
     9301 atacttaaaa ggaacattct tgaatttacg ataaatacag ttagtacaat cgctgatatc
     9361 ggtgtacctg ctataaacaa cgcgcacata ataaattgcg cagcaccggc atatataaca
     9421 agacataaca agataatttc taaaatacta aagttttgag acgaagccac aataccaaat
     9481 gaaataccaa caccggcata acccaataat gttgggatac actcttgtac gccttgtcta
     9541 aaacttaaat gtgttgccat ctcaattacc tcctttgcca ctttatattt atataattac
     9601 gaaaaaataa tatacctagt ctaatacagt tctataccct aagcaataac attagacacc
     9661 agtttgtctg aggttagaca tactggcgtc ttcaatcatc ttcatatcaa ctttttattt
     9721 ttgataattt tcaattttta tttaagacta cctccatatt ttctacg
//
LOCUS       DANBWH010000099         9646 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.102, whole genome shotgun sequence.
ACCESSION   DANBWH010000099 DANBWH010000000
VERSION     DANBWH010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9646
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.102"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            40..3492
                     /locus_tag="PPY92_001651"
     CDS             40..3492
                     /locus_tag="PPY92_001651"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDJ2625645.1"
                     /translation="MKQIKKLLVANRGEIAIRIFRAAAELDISTVAIYSNEDKSSLHR
                     YKADESYLVGSDLGPAESYLNIERIIDVAKQANVDAIHPGYGFLSENEQFARRCAEEG
                     IKFIGPHLEHLDMFGDKVKARTTAIKADLPVIPGTDGPIKSYELAKEFAEEAGFPLMI
                     KATSGGGGKGMRIVREESELEDAFHRAKSEAEKSFGNSEVYIERYIDNPKHIEVQVIG
                     DEHGNIVHLFERDCSVQRRHQKVVEVAPSVGLSPTLRQRICDAAIQLMENIKYVNAGT
                     VEFLVSGDEFFFIEVNPRVQVEHTITEMVTGIDIVKTQILVAAGADLFGEEINMPQQK
                     DITTLGYAIQCRITTEDPLNDFMPDTGTIIAYRSSGGFGVRLDAGDGFQGAEISPYYD
                     SLLVKLSTHAISFKQAEEKMVRSLREMRIRGVKTNIPFLINVMKNKKFTSGDYTTKFI
                     EETPELFDIQPSLDRGTKTLEYIGNVTINGFPNVEKRPKPDYELASIPTVSSSKIASF
                     SGTKQLLDEVGPKGVAEWVKKQDDVLLTDTTFRDAHQSLLATRVRTKDMINIASKTAD
                     VFKDGFSLEMWGGATFDVAYNFLKENPWERLERLRKAIPNVLFQMLLRASNAVGYKNY
                     PDNVIHKFVQESAKAGIDVFRIFDSLNWVDQMKVANEAVQEAGKISEGTICYTGDILN
                     PERSNIYTLEYYVKLAKELEREGFHILAIKDMAGLLKPKAAYELIGELKSAVDLPIHL
                     HTHDTSGNGLLTYKQAIDAGVDIIDTAVASMSGLTSQPSANSLYYALNGFPRHLRTDI
                     EGMESLSHYWSTVRTYYSDFESDIKSPNTEIYQHEMPGGQYSNLSQQAKSLGLGERFD
                     EVKDMYRRVNFLFGDIVKVTPSSKVVGDMALYMVQNDLDEQSVITDGYKLDFPESVVS
                     FFKGEIGQPVNGFNKDLQAVILKGQEALTARPGEYLEPVDFEKVRELLEEEQQGPVTE
                     QDIISYVLYPKVYEQYIQTRNQYGNLSLLDTPTFFFGMRNGETVEIEIDKGKRLIIKL
                     ETISEPDENGNRTIYYAMNGQARRIYIKDENVHTNANVKPKADKSNPSHIGAQMPGSV
                     TEVKVSVGETVKANQPLLITEAMKMETTIQAPFDGVIKQVTVNNGDTIATGDLLIEIE
                     KATD"
     gene            complement(3632..4543)
                     /locus_tag="PPY92_001652"
     CDS             complement(3632..4543)
                     /locus_tag="PPY92_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HDJ2625646.1"
                     /translation="MFGKKNLKWLGVVATLMMTFVQLGGALVTKTGSADGCGSSWPLC
                     HGALIPEFFPIDTIIELSHRAVSALSLLMVLWLVITAWKHIGYIKEIKPLSIISVGFL
                     LLQALIGAAAVIWQQNDYVLALHFGISLISFSSVFLITLIIFSIDQKYEADELYIKKP
                     LRRLTWLMAIIIYCGVYTGALVRHADASLAYGGWPLPFHDLVPHSEQDWVQLTHRIMA
                     FIVFTIIMITYIHAVKNYPNNRTVHYGYTAAFILVILQVITGALSIMTNVNLIIALFH
                     ALFITYLFGMTTYFIMLMLRSVRSDKQ"
     gene            4983..5894
                     /locus_tag="PPY92_001653"
     CDS             4983..5894
                     /locus_tag="PPY92_001653"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HDJ2625647.1"
                     /translation="MSKEHTLSQNISRVNFKELQQIIKMGLVQGNLIPAFAGAWLAVV
                     MTNHSFLSSIPQILLMLFGSTLIMGGACALNNYYDQDIDRIMPSKQNRPTVNNRITDQ
                     NLLLLSFGMMLVGEICLFLLNIPSGVLGLMGIVGYVSYYSIWSKRHTTWNTVIGSFPG
                     AVPPLIGWVAIEGQISLTAIALFLVVFCWQPIHFYALAIKRKDEYALANIPMLPSVKG
                     FKRTRVSMFIWLIILLPVPLLLINLGVVFVVLATLLNLGWIALGLTTFKKNSDQTKWA
                     TQMFIYSLNYLVIFFVLAVIVSLLTLI"
     gene            5919..6380
                     /locus_tag="PPY92_001654"
     CDS             5919..6380
                     /locus_tag="PPY92_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HDJ2625648.1"
                     /translation="MGVPILPTISTTCIVISAILIAIGWRLIWKREINKHKNVMLAAA
                     VFALTFFLIYASRTIFIGNTAFGGPASIKKYYTIFLFFHINLATIGGILGLVQIITAF
                     KDKYNVHRKFGPFASVIWFCTAITGVAVYLLLYVLYPGGETTSLIKATFGH"
     gene            6707..7744
                     /locus_tag="PPY92_001655"
     CDS             6707..7744
                     /locus_tag="PPY92_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HDJ2625649.1"
                     /translation="MKKLIIRVVGVLFLVGFLIYLFYSPRLKFDVLENPNKGNKVNRS
                     EQVNKSNNHAENPKPKEGVGTWVGKDIKVLTSKFGQADRVYPFRDGYKNYVFKDKNSY
                     YIVSTKREEIVSVYATGEKVNVSPLKIGQHSAEIFNHTSINPEPSFKVDGKKYEFELS
                     DEDLKTQTLIKYGDIYAQVYSDQQSKKVLSVRFLTKEMLADIEPYQLNSNSTSEEHNK
                     RPVEQNPNQLISLYEVTNEMRKLKGLKPLKINSDLAHIASNNLYEATSNGSDSVEFTE
                     DALRGQLDKNHVTYKTTAQNVGYAFNDVPTLIHSWMNSDIHRSRLLNSKYDEMGGDVM
                     RDYYSLIFLEK"
     gene            7760..8194
                     /locus_tag="PPY92_001656"
     CDS             7760..8194
                     /locus_tag="PPY92_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDJ2625650.1"
                     /translation="MINEASLAILDDIDELADMIVASDIYASFEQAKQALENNDEAHL
                     LYQSFLKSKEKYDEVMRFGKYHPDYKKVMLETRQRKRAYEMLDVVMHYKAREMALQHL
                     IDEVVTKIAYAVSEHVKIETGNPFFQTSHSGCATGGSCNCSL"
     gene            complement(8257..9183)
                     /locus_tag="PPY92_001657"
     CDS             complement(8257..9183)
                     /locus_tag="PPY92_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDJ2625651.1"
                     /translation="MKKVNRNILNAVLVGAGFLSSLLMINKNKVITKKQTIPAFFKGN
                     APYIFAHRGGMALRPEQTQLAFDYAKQLGVDGFETDVRLTKDQQLIVFHDATVDRTTN
                     GSGKVSAHTLAELKKLDAAYHFKDINGLTPYRGHAHTTILTFDELLKQYPDMYINVDL
                     KDAPESYEGSIAPQIMFDTIAENQAFDRVLVTSFYKEQIVRFNKIAQGSVAIGASQQE
                     VTEAFLKYHLLGGRYYQPLAQTFQMPTHFKGIDLTSSRFIKWLNDMNIIPGYYGVNSI
                     DLMNDLYQKGAHTIVTDRPDLAQQFKQTIPNK"
     gene            9422..>9646
                     /locus_tag="PPY92_001658"
     CDS             9422..>9646
                     /locus_tag="PPY92_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2129 domain-containing protein"
                     /protein_id="HDJ2625652.1"
                     /translation="MNLIPRTSIVVYLKHMKHERQIRKYGHIVHSNRDRKFVIMYVNE
                     QDVDQIVHKLMQLKYVRHIDGSPYKYLKKTY"
BASE COUNT         3365 a         1423 c         1764 g         3094 t
ORIGIN      
        1 tagttagaaa attctaatag tttgaggagt gtatcgctat tgaaacaaat aaaaaagtta
       61 cttgttgcta accgtggaga aattgcaatt cgtatattca gagcggcggc agaattagac
      121 atcagcacag ttgcaattta ttcgaatgaa gacaaaagtt cattacatag atataaagca
      181 gatgaatcct atttagttgg aagtgattta ggtcctgctg aaagttattt aaatattgag
      241 cgtatcattg atgtagcaaa acaagcgaat gtggatgcga ttcatcctgg ctatggattt
      301 ttaagtgaaa atgaacaatt tgcgcgtcgt tgtgcagaag aaggaattaa atttattggt
      361 cctcatttag aacatttaga tatgtttgga gataaagtta aagctcgtac aacggctatc
      421 aaggcagatt taccagttat tcctggtaca gacggtccaa ttaaatcata tgaattagca
      481 aaagaatttg cagaagaagc tggtttcccg ctaatgatta aagccacaag tggtggcggc
      541 ggtaaaggta tgagaatcgt tcgtgaagaa agtgaattag aagatgcttt ccatagagca
      601 aaatcagaag ctgaaaaatc atttggtaat agtgaagttt acatagaaag atacattgat
      661 aatccaaagc atattgaagt acaagtcata ggtgacgaac atggaaatat cgtacactta
      721 tttgaacgtg attgttcagt acaacgtcgt catcaaaaag ttgtagaagt tgcaccatca
      781 gttggattat caccaacatt acgtcaacgt atttgtgatg ctgcaattca attgatggaa
      841 aatattaaat atgtcaatgc aggtactgtt gaatttctag tatctggtga cgaattcttc
      901 tttatagaag ttaaccctcg tgtacaagta gagcatacaa ttacagagat ggtaacagga
      961 attgatattg ttaagacaca aattttagtt gcagcaggtg ccgatttatt tggtgaagag
     1021 attaatatgc cgcaacaaaa agatattaca acattaggct atgccatcca atgtcgtatt
     1081 acaacagaag atccgttaaa tgatttcatg ccggatactg gaacaatcat tgcttatcgt
     1141 tcaagcggtg gctttggtgt acgtcttgat gctggagatg gtttccaagg tgctgagata
     1201 tcaccttatt atgattcatt actcgtaaaa ttatctacac atgcgatatc atttaaacaa
     1261 gcagaagaaa aaatggtacg ttcattacga gaaatgcgta ttcgtggtgt taaaactaat
     1321 attccattct taattaatgt aatgaagaat aaaaagttca caagtggtga ttacacaact
     1381 aaatttattg aagaaacacc agaacttttc gacattcagc cgtctctaga tagaggtact
     1441 aaaacattag aatatatagg taatgtaaca attaatggtt tcccaaatgt tgagaaacgt
     1501 ccgaaaccag actatgaatt agcatcaatt ccaactgtat cttcaagtaa aatcgcttca
     1561 tttagtggta cgaaacaatt gcttgatgaa gtaggtccaa aaggtgtagc tgaatgggtt
     1621 aaaaagcagg atgatgtctt actaacagat acaaccttta gagatgcaca ccaatcatta
     1681 ttagctacac gagttagaac taaggatatg attaatatcg catccaaaac agcggacgta
     1741 tttaaagatg gtttctcact agaaatgtgg ggcggtgcta catttgatgt ggcatataat
     1801 ttcttgaagg aaaacccatg ggaacgactt gaacgtctac gtaaagctat cccaaatgta
     1861 ttattccaaa tgttgttacg tgcttcaaac gcagttggtt ataaaaacta tcctgataat
     1921 gttattcata aattcgtaca agaaagtgct aaagcaggca tagatgtctt tagaattttc
     1981 gattcattaa actgggtaga tcaaatgaaa gttgccaatg aagcagtaca agaagcgggc
     2041 aaaatctctg aaggtactat ttgttataca ggtgacattt taaatcctga gcgatcaaac
     2101 atttatactt tagagtatta tgtcaaacta gctaaagagt tagaacgtga aggtttccat
     2161 attttagcga ttaaagatat ggcaggctta ttaaaaccta aagccgctta cgaattgatt
     2221 ggtgagttaa aatcagctgt agatttacca attcatcttc acactcatga tacaagtggt
     2281 aatggtttat taacatacaa acaagcaata gatgctgggg tcgatatcat tgatactgct
     2341 gttgcttcaa tgagtggttt aacaagtcag ccaagcgcca attcgttata ttatgcatta
     2401 aatggcttcc cacgccacct tagaactgat attgaaggta tggagtcact tagtcattat
     2461 tggtcaactg tacgtactta ttattcagac tttgaaagtg atatcaaatc accgaatact
     2521 gaaatttatc aacatgaaat gcctggtgga cagtattcga atttaagtca acaagctaaa
     2581 agtttaggtt taggcgaaag atttgatgaa gtcaaagata tgtatcgcag agtgaatttc
     2641 ttatttggtg atatcgtaaa agtaactcca tcgtctaaag tagttggtga tatggcactt
     2701 tatatggtac aaaatgatct tgatgaacaa tccgtgatta cagatggcta taaattagat
     2761 ttcccagaat cagtagtgtc gttcttcaaa ggcgaaatag gacaacctgt aaatggtttt
     2821 aataaagatt tacaagcggt tattttaaaa ggccaagaag cactaacagc tcgtccaggt
     2881 gaatatctag agccagttga ttttgaaaaa gtccgtgagt tgcttgaaga agagcaacaa
     2941 ggtcctgtta cggagcaaga tattattagt tatgtattat atccaaaagt atatgaacaa
     3001 tatattcaaa ctagaaatca atacggaaac ttatcgttac ttgatacgcc tacattcttc
     3061 tttggaatgc gtaatggtga aacagtagaa atcgaaatcg ataagggtaa acgattaatt
     3121 attaaactag aaacgattag tgaaccagat gaaaatggta atagaacgat ttactatgcg
     3181 atgaatggtc aagcgagacg tatttacatt aaagatgaaa atgtgcatac aaatgcgaac
     3241 gttaagccaa aagcagataa gagtaatcca agtcatatcg gtgctcaaat gccaggttca
     3301 gtaactgaag tcaaggttag tgttggtgaa actgtgaaag ctaatcagcc gttgctaatt
     3361 actgaagcta tgaaaatgga aacaacaatt caagcaccat ttgacggtgt gattaaacaa
     3421 gtaactgtaa ataatggtga cacaatagcg acaggcgatt tattaatcga aattgaaaaa
     3481 gcaactgact aaaatgatta aaataaaacg agattacaca acaattgacg tgtaatctcg
     3541 tttttacatg gcttaggttt aattttaaat attgatataa aaaaccgtaa ttttaatggg
     3601 cgtgccatta aaattacggt ttgctttttt gttattgctt gtcacttctt actgatcgta
     3661 acattagcat gataaagtat gttgtcatac caaataaata agtgataaat aatgcatgga
     3721 aaagtgcgat tatcaggtta acatttgtca taatagataa tgcacctgtg ataacttgta
     3781 atataacaag tataaatgca gctgtataac cataatgaac agtacgatta tttggataat
     3841 ttttaacagc gtgaatataa gtaatcataa taatcgtaaa cactataaaa gccatgatac
     3901 gatgcgtgag ttgaacccaa tcttgttctg aatgtggtac aagatcgtgg aatggcaatg
     3961 gccaaccacc atatgctaaa cttgcatccg catgtctcac tagtgcacca gtataaacac
     4021 cacaataaat gatgattgcc attaaccatg ttaaacgtct taatggcttt ttgatatata
     4081 attcgtcagc ttcatatttt tgatctatag agaaaataat caatgttatt aaaaatacag
     4141 atgagaaact gattaatgat ataccaaagt gcaatgctaa aacgtaatcg ttttgttgcc
     4201 aaataacagc ggcagctccg attaatgctt gcaataataa gaatccaaca ctaatgattg
     4261 ataaaggttt aatttcttta atatagccta tatgtttcca tgcagtgata actaaccata
     4321 agaccattaa taaagacaaa gctgaaacgg ctctatgact taactcaata atcgtatcaa
     4381 taggaaagaa ttctggaatc aacgcaccat gacatagtgg ccaagaagaa ccacaaccat
     4441 cagctgatcc ggttttggta actaaggctc caccaagttg tacaaatgtc atcattaacg
     4501 ttgctacgac acctaaccat tttaaattct ttttgccaaa caatttatac accccatcat
     4561 aaaaaaacgc atcactgaga attagaatta atagccggat ttgagatgat actataaatt
     4621 gacctgattg taggatatga aaagatggga tccaacgcag gaaatatgat ttaattttac
     4681 aagtattgta tgtggacgac ataaaaagaa cttaatcgta atgatttttt tattgggata
     4741 attatgtaat ccacgtttac tcaaatttga agcttaaata ttaatctctt ttagatgcat
     4801 gattctactt attaaaattt tctaacatat acattatagc aataaattaa atgttatttg
     4861 tgtcacaaaa ttgacatttt ctcttgtcgt taatttgtca cattttaatt ttattaaacc
     4921 tagtcttttt atgcaatcta agatatcata atcttatatg aacaaattta aggaggggga
     4981 ttatgagcaa agagcatact ttgtcacaaa atattagcag agttaacttc aaagaattgc
     5041 aacagataat taaaatggga cttgttcaag gtaacttaat tccggctttt gcgggcgcat
     5101 ggctagcagt tgtaatgaca aatcattcct tcttatcatc aatacctcaa attttattaa
     5161 tgctatttgg atctacttta attatgggtg gcgcatgtgc gttaaataat tattacgacc
     5221 aagatattga tcgtattatg cctagtaaac aaaatagacc aactgtaaat aatagaatta
     5281 cagatcaaaa tttattacta ttaagttttg gtatgatgtt agttggagaa atttgtttgt
     5341 ttttattgaa tataccatca ggcgtacttg gtcttatggg gattgtaggt tatgtgtctt
     5401 attactcaat atggtctaaa agacatacaa catggaacac agtgattggg agttttcctg
     5461 gagcagtacc accactaatt ggatgggttg caattgaagg acaaattagt ttaacagcga
     5521 ttgcgctgtt tttagttgta ttttgttggc aaccaattca tttttatgcc ttagctatta
     5581 aacgtaaaga tgaatatgca cttgcaaata ttccaatgtt accatcagtt aaaggcttta
     5641 aacgtacacg tgtcagtatg tttatctggt tgattatttt attgccagta cctttattac
     5701 taataaattt aggtgtcgta ttcgtagtgt tagctacatt attaaattta ggatggattg
     5761 cattaggttt aacaacattt aagaaaaatt cagatcaaac aaaatgggca acacaaatgt
     5821 ttatatattc actaaattat ttagtgatct ttttcgtgtt agctgtaatt gtttcattac
     5881 ttactttgat ctagattaat taagttagga tgaaaaatat gggcgttcca attttaccaa
     5941 cgattagtac gacatgtatt gtcattagtg caattttaat tgccattggt tggagactta
     6001 tttggaaaag ggaaataaat aaacacaaaa atgttatgtt agctgcagct gtttttgctt
     6061 taactttttt cttaatctat gcaagtagaa cgatttttat cggtaataca gcatttggcg
     6121 gaccagcatc aattaagaaa tattatacga ttttcttatt tttccacatt aatttagcaa
     6181 caattggtgg tattctaggc ctagttcaaa ttattactgc atttaaagat aaatataatg
     6241 tacaccgcaa attcgggcca ttcgcttcag ttatatggtt ctgtactgca attacaggtg
     6301 tagcagttta cttattatta tatgtattat atccaggtgg agaaacgaca tcactgatta
     6361 aagcaacatt tggtcattaa tatgcttata caaatgatac taatctgata aggcaatttg
     6421 ttttatcagg ttttttgatg aagaatagtc ggacagttga tggtattcat ttgatacctt
     6481 aactgtccat ttttttatac atattttaat cttaataatg aaattcaacc tgaaaacata
     6541 aagataggtt atgatataga gtgtagggaa aatctttatg acgttaaaat tatgcctgaa
     6601 gatgattttg ttttatcgaa acgacacagt tatgtcagtg ttgaaaattt gctaattaat
     6661 gtgttgtttt ttacaatagt attaatactt tttaggtggt tgattgatga aaaagttaat
     6721 tataagagtc gtcggtgtat tatttttagt tggattttta atatatcttt tttattcacc
     6781 gagattaaaa tttgatgtat tagagaaccc gaataaaggt aataaagtaa atagatctga
     6841 acaggtgaat aaatcaaata accatgctga aaatccaaag cctaaagaag gtgttggtac
     6901 atgggtaggt aaagatatta aagtgcttac ttctaaattt ggacaagcag atcgtgtcta
     6961 cccttttaga gatggttaca aaaattatgt gtttaaagac aaaaacagtt attacattgt
     7021 ttcaactaaa cgtgaagaaa tcgtttcagt gtatgctaca ggtgagaaag tcaatgttag
     7081 tccgttaaaa ataggacaac attctgcaga aatttttaat catacaagta ttaatccaga
     7141 accttccttt aaagttgatg gtaaaaaata tgaatttgaa ctttcagatg aagatttaaa
     7201 aacacaaaca ctgattaaat atggcgacat atatgcacaa gtgtattctg atcaacaatc
     7261 gaaaaaggtg ctaagtgtac gatttttaac aaaagaaatg ttagcagata ttgaacctta
     7321 tcaattaaat tctaattcta cgtcagaaga gcataataag cgtccagttg agcaaaatcc
     7381 aaatcaatta atttctcttt atgaagtaac gaatgaaatg agaaaattaa aaggattaaa
     7441 accattgaaa atcaatagcg atttagcaca tattgcatct aataacttat atgaagcgac
     7501 ctctaatggt tctgatagtg ttgaatttac agaggacgca ttaagagggc aattagataa
     7561 aaatcacgtt acttataaaa caactgctca aaatgttggt tatgcgttta atgatgtacc
     7621 aacattaatc catagttgga tgaattcaga tatacatcga tctcgtctat taaattcaaa
     7681 atacgatgag atgggtggag atgtaatgag agattattat tcactaattt tcttagaaaa
     7741 ataacaatga ggggtattta tgattaatga agcttcacta gcgatattag atgatattga
     7801 tgaactagct gatatgatag ttgcatcaga tatttatgca tcatttgagc aagctaaaca
     7861 ggcgctcgaa aacaatgacg aagcacattt actttatcaa tcatttttaa aatcaaaaga
     7921 gaaatatgat gaagtaatgc gtttcgggaa atatcatcct gattataaga aagtcatgtt
     7981 agagacaaga caacgcaaaa gagcatatga gatgcttgac gttgtgatgc attacaaagc
     8041 tagggaaatg gcacttcaac atttaattga tgaagttgtt acgaaaattg cgtacgctgt
     8101 ttcagagcat gtcaaaatag aaacaggtaa tccattcttt caaacatcac atagtggttg
     8161 tgcgacgggc ggatcctgta attgttcatt ataaaaaaca tcgagtcaga aaaaggtggt
     8221 tattgaaacc actaactagc atctgactcg atgtttttat ttattcggga ttgtttgttt
     8281 gaattgttgt gctaaatctg gtcgatctgt cacaatcgtg tgtgcacctt tttggtataa
     8341 atcattcatc agatctatac tatttacgcc ataatagcct ggaatgatat tcatatcatt
     8401 taaccatttg ataaaacgag atgaagtcaa atcaatgcct ttaaaatgag taggcatttg
     8461 gaacgtttgt gctaatggtt ggtagtacct accacctaat aaatgatatt ttaaaaatgc
     8521 ttctgtaact tcctgttggc tagcaccaat tgcgacggat ccttgtgcaa ttttattaaa
     8581 acgaacgatt tgttctttat aaaaacttgt cacaagaacg cggtcaaatg cttgattttc
     8641 tgcaattgta tcaaacataa tttgtggtgc gattgagcct tcataggatt caggagcatc
     8701 ttttaagtct acgtttatat acatatcagg atattgcttc agcaactcat cgaaggttag
     8761 tatagttgtg tgtgcatgac cacgatatgg tgttagtcca ttgatatctt tgaagtgata
     8821 agctgcgtct aatttcttta attctgctaa tgtgtgggca ctaacttttc cagagccgtt
     8881 cgtcgttcta tcaacagttg cgtcatgaaa aacgataagc tgttgatctt ttgtgagtct
     8941 cacatctgtt tcaaagccat caacgcctaa ttgtttagca tagtcaaatg caagttgcgt
     9001 ttgctctggt cttaaagcca taccaccgcg atgcgcaaat atatatggtg cattgccttt
     9061 gaaaaaagca gggatggttt gctttttagt aatcacttta tttttattga tcattaatag
     9121 actacttaaa aatccagcac cgactagtac cgcatttaaa atgtttctgt ttactttttt
     9181 catagaaaat tcctcctcgt ttacttttat agcttaacaa aatcatagtt ctgactacag
     9241 cgaaatgtgt ggttaatcat atagtgatgc tcttgagaat tgaattaata taatgatgtt
     9301 taatataatc taaatacatt tatcgagcaa ttacataaga cataattaag gtagcttaat
     9361 attttaaaat taataaagac atgatatgat taatgctgaa aataaatgga gtgaggttta
     9421 aatgaattta atcccaagaa ctagtattgt agtttattta aaacatatga aacatgaacg
     9481 acaaatccga aaatatggac atatcgttca ttcaaataga gatcgtaaat ttgtaattat
     9541 gtatgtgaat gagcaggatg ttgatcaaat tgtacataaa ctaatgcaac ttaaatacgt
     9601 tagacacatt gatggctcac catataaata cttaaagaaa acttac
//
LOCUS       DANBWH010000100         9641 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.103, whole genome shotgun sequence.
ACCESSION   DANBWH010000100 DANBWH010000000
VERSION     DANBWH010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9641
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.103"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            116..610
                     /gene="tpx"
                     /locus_tag="PPY92_001659"
     CDS             116..610
                     /gene="tpx"
                     /locus_tag="PPY92_001659"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDJ2625653.1"
                     /translation="MTEITFKGGPIHLKGQQINEGDFAPDFTVLDNDLNQVTLADYAG
                     KKKLISVVPSIDTGVCDQQTRKFNSEASKEEGIVLTISADLPFAQKRWCASAGLDNVI
                     TLSDHRDLSFGENYGVVMEELRLLARAVFVLDVDNKVVYKEIVSEGTDFPDFDAALAA
                     YKNI"
     gene            733..1680
                     /locus_tag="PPY92_001660"
     CDS             733..1680
                     /locus_tag="PPY92_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDJ2625654.1"
                     /translation="MAEQQTIMERLFHTLDEKAKTLNNENGQSFIENLGLAMEQVYTN
                     ERGLLEQSTLQDRRKAFQFAYLSLMQEEKIQANHQITPDSIGLILGFLVERFMNNQEE
                     LHIVDIASGAGHLSATVKEVLPEIAVMHHLIEVDPVLSRVSVHLANFLEIPFDVYPQD
                     AIMPLPLEEADIVIGDFPVGYYPIDERSKEFKLGFEEGHSYSHYLLIEQAINALKDAG
                     YAFLVVPSNIFTGEHVKQLEKYIATETEMQAFLNLPPTLFKNEKARKSILILQKKKSG
                     ETKPVEVLLANIPDFKNPSQFQGFMTELNQWMDTNRPKK"
     gene            1768..2970
                     /locus_tag="PPY92_001661"
     CDS             1768..2970
                     /locus_tag="PPY92_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDJ2625655.1"
                     /translation="MSKLILAINAGSSSLKFQLIRMPEEELVTKGLIERIGLKDSIFT
                     IEVNGEKVKTVQDIKDHVEAVDIMLDAFKAHNIINDINDIDGTGHRVVHGGEKFPESV
                     AITDEVEKEIEELSELAPLHNPANLMGIRAFRKLLPNIPHVAIFDTAFHQTMPEKAYL
                     YSLPYHYYKDYGIRKYGFHGTSHKFVSQRAAEMLDKPIEDLRIISCHIGNGASIAAID
                     GGKSIDTSMGFTPLAGVTMGTRSGNIDPALIPFIMEKTGKTAEQVLEILNKESGLLGL
                     SGTSSDLRDLSEEAESGKARSQMALDVFASKIHKYIGSYAARMHGVDVIVFTAGIGEN
                     SVEIRAKVLEGLEFMGVYWDPKKNENLLRGKEGFINYPHSPVKVVVIPTDEESMIARD
                     VMTFGGLK"
     gene            complement(3216..3716)
                     /locus_tag="PPY92_001662"
     CDS             complement(3216..3716)
                     /locus_tag="PPY92_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDJ2625656.1"
                     /translation="MITYKNILIAVDGSHEAEWAFNRAVGVAKRNDAKLTIVNVIDSR
                     TYSSYEVYDAQFTEKSKHFAEELLNGYKEVATNAGVKDVETRLEFGSPKSIIPKKLAH
                     EINADLIMSGTSGLNAVERFIVGSVSESIVRHAPCDVLVVRTEELPADFQPQVATTQL
                     REKYQN"
     gene            3857..4975
                     /gene="ald"
                     /locus_tag="PPY92_001663"
     CDS             3857..4975
                     /gene="ald"
                     /locus_tag="PPY92_001663"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HDJ2625657.1"
                     /translation="MKIGIPREIKNNENRVGLSPSGVHALVESGHTVLVETNAGSGSF
                     FEDVDYKEAGAEIVAEQAKVWDVDMVIKVKEPLESEYPYFKEGLVLFTYLHLANEEKL
                     TQALIDRKVISIAYETVQLPDRSSPLLSPMSEVAGRMSAQVGAEFLQKLNGGMGILLG
                     GVPGVPKGKVTIIGGGQAGTNAAKIALGLGADVTILDVNPKRLQQLDDLFGGRVHTIM
                     SNPLNIELYVKQSDLVIGAVLIPGAKAPRLVTEDMIKQMKNGSVIIDIAIDQGGIFET
                     TDKITTHDDPTYIKHGVVHYAVANMPGAVPRTSTLALNNATLPYALMLANKGYREAFK
                     SNQPLSLGLNTYKGHVTNKGVAEAFEMEYKSVEEALQL"
     gene            5099..5197
                     /locus_tag="PPY92_001664"
     CDS             5099..5197
                     /locus_tag="PPY92_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625658.1"
                     /translation="MNDIDLIHYMLLMNIPAYVFLACLLHIAFRLG"
     gene            complement(5291..6346)
                     /locus_tag="PPY92_001665"
     CDS             complement(5291..6346)
                     /locus_tag="PPY92_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDJ2625659.1"
                     /translation="MTKISKIIDELNNQQADAAWITTPLNVYYFTGYRSEPHERLFAL
                     LIKKDGKQVLFCPKMEVEEVKASPFTGEIVGYLDTENPFSLYPQTINKLLIESEHLTV
                     ARQKQLISGFNVNSFGDVDLTIKQLRNIKSEDEISKIRKAAELADKCIEIGVSYLKEG
                     VTEREVVNHIEQTIKQYGVNEMSFDTMVLFGDHAASPHGTPGDRRLKSNEYVLFDLGV
                     IYEHYCSDMTRTIKFGEPSKEAQEIYNIVLEAETSAIQAIKPGIPLKDIDHIARNIIS
                     EKGYGEYFPHRLGHGLGLQEHEYQDVSSTNSNLLEAGMVITIEPGIYVPGVAGVRIED
                     DILVTNEGYEVLTHYEK"
     gene            6521..7210
                     /locus_tag="PPY92_001666"
     CDS             6521..7210
                     /locus_tag="PPY92_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDJ2625660.1"
                     /translation="MKLSFHGQSTIYLEGNNKKVIVDPFISNNPKCDLNIETVQVDYI
                     VLTHGHFDHFGDVVELAKKTGATVIGSAEMADYLSSYHGVENVHGMNIGGKANFDFGS
                     VKFVQAFHSSSFTHENGIPVYLGMPMGIVFEVEGKTIYHTGDTGLFSDMSLIAKRHPV
                     DVCFVPIGDNFTMGIDDASYAINEFIKPKISVPIHYDTFPLIEQDPQQFKDAVNVGDV
                     QILKPGESVQF"
     gene            complement(7515..7928)
                     /locus_tag="PPY92_001667"
     CDS             complement(7515..7928)
                     /locus_tag="PPY92_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDJ2625661.1"
                     /translation="MYKNILLGVDTQLKNEKALKEVSKLAGEGTVVTVLNAISEQDAQ
                     ASIKAGVHLNKLTEERSKRLEKTRKALEDYGIDYDQIIVRGNAKEELLKHANSGKYEI
                     VVLSNRKAEDKKKFVLGSVSHKVAKRATIPVLIVK"
     gene            8133..9431
                     /locus_tag="PPY92_001668"
     CDS             8133..9431
                     /locus_tag="PPY92_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDJ2625662.1"
                     /translation="MTKHEQILDYIESLSIGSKISVRKIAKFLNVSEGTAYRAIKDAD
                     KMGMVATIDRVGTVRIEKRNRNEIEHLTFNEIVNIIDGQVLGGNKGITKMVSKFAIGA
                     MELKDILKYIGPKTLLIVGNREDVQIEALKRGTAILITGGFKPSNKVINFANEHDLPV
                     LSSSYDTFLVANIINKALFNQKIRKDILIVQDIMTPLDDLSVLFDTMKIADYKRMANR
                     TGHTRFPVVNESYKLVGIVTSREMINTKDDDEIDKVMTRNPIYVNAMSTVASCAHMMI
                     WEGIELIPVVSSNKKTVGVINRQDVLKSMQLLGRQPQMGETINDQIAKYITMNQDGIT
                     VEVSPLLINHYGTVSKAAFVSIIEETIQYEMRKFKKGNVMIENLNIVYIKTVPIESHI
                     TVRFGILDVGRNFAKIEVNMHSQNDKVASALVICQMFDEV"
BASE COUNT         3257 a         1438 c         1772 g         3174 t
ORIGIN      
        1 attcaacaat ttgtataaaa ttaaatttgc ttttgattaa agcatgaaaa ttgtaatcaa
       61 accataaatt gtcgtatgat gtagttagaa ttttaaaatg caggaggtca agtatatgac
      121 tgaaataaca ttcaaaggtg gaccaatcca cttaaaaggt caacaaatta atgaaggtga
      181 ttttgcacct gattttacag tgttagataa tgacttaaat caagtaacat tagcagatta
      241 tgctggtaaa aagaaattaa ttagtgtggt accatcaatt gatacaggtg tttgtgatca
      301 gcagactcgc aaattcaact ctgaagcttc taaagaagag gggattgtgc ttacaatttc
      361 agcagactta ccattcgcac aaaaaagatg gtgcgcttca gcaggtttag acaatgtcat
      421 tacattaagt gaccaccgtg acttatcatt tggtgaaaac tatggcgttg ttatggaaga
      481 acttcgctta ttagctcgtg cagtatttgt attagatgta gataataaag ttgtttataa
      541 agaaatcgtt agtgaaggta ctgatttccc agattttgat gctgctttag ctgcatacaa
      601 aaatatttaa tcattaaaga gataaatctt aaaatgtata catcgtgtcc atcgttgtca
      661 acagcattaa aatagaattg ttttctatga ttgctaagac ctatgggcac tttttattgg
      721 agagggacga atatggcaga acaacaaaca attatggaac gcttgtttca tacattagat
      781 gaaaaagcta aaacattaaa taatgaaaat ggccaaagtt ttattgaaaa tcttgggcta
      841 gcaatggaac aagtatatac caatgaaaga ggattgttag aacaatcgac gttacaagat
      901 cgacgtaaag cattccaatt tgcatattta agtttaatgc aggaagaaaa gatacaagca
      961 aatcatcaaa ttacaccaga ttcaattgga ttgatactag gatttttagt tgagcgtttt
     1021 atgaacaacc aagaagaatt acatattgtt gatattgcaa gtggtgccgg tcatttaagt
     1081 gctactgtaa aagaagtgtt acctgaaatt gcggttatgc atcatttaat tgaagttgat
     1141 ccagttttat cacgtgttag tgtacattta gcaaacttct tagaaattcc tttcgatgtg
     1201 tatcctcaag atgccatcat gccactacca ttagaagaag cagatatcgt tattggtgat
     1261 tttccagtag gctattatcc aattgatgaa agaagtaagg agtttaagct aggttttgaa
     1321 gaaggacata gttattcaca ttatttatta atagaacaag caataaatgc attaaaagat
     1381 gctggatatg cctttctagt ggtaccaagt aatattttta caggtgaaca tgtaaaacag
     1441 cttgaaaaat atattgcaac agagacagag atgcaagcat ttttaaattt accaccaact
     1501 ttatttaaaa atgaaaaagc gcgaaaatct atattaattt tacaaaagaa aaaatcgggt
     1561 gaaacaaagc cagttgaagt attattggca aatattcctg atttcaaaaa tccttcacaa
     1621 tttcaaggat ttatgacaga gttaaatcag tggatggaca caaatcgtcc taaaaaataa
     1681 tctgtaatag tatagtcata aaactgtatg aatgataaaa tgagaaatga aatagtataa
     1741 aaagcaaacg atggaggatc attacttatg tcaaaattaa tcttggctat caatgctggt
     1801 agttcatcat taaaatttca attaattaga atgcctgaag aggaattagt aacaaaaggt
     1861 ttaatagaaa gaatcggatt gaaggattca atttttacaa ttgaagtcaa cggggaaaaa
     1921 gttaaaacag tacaagatat caaggatcac gttgaggcag ttgatatcat gttggatgct
     1981 tttaaagcgc ataatattat taatgatatt aacgatatcg atggaacagg tcaccgtgtt
     2041 gtacatggtg gtgaaaaatt ccctgaatca gtagctatta ctgatgaggt tgaaaaggaa
     2101 attgaagaat taagcgaatt agcaccgctt cacaacccag ctaacttgat gggtattcgt
     2161 gctttccgta agttattacc aaatatccct catgtggcaa tttttgatac agcattccat
     2221 caaacaatgc ctgaaaaagc atatctatat agcttgccat atcattatta taaagattat
     2281 ggcattcgta agtatggttt ccatggtaca agccataaat ttgtatcaca aagagcggca
     2341 gaaatgttag ataagccaat tgaagattta cgtattattt catgtcacat tggtaatgga
     2401 gcatcaattg cagctatcga tggcggtaaa tctattgata catcaatggg cttcacacca
     2461 cttgctggtg taacaatggg tactcgttct ggtaatattg accctgcttt aattccattt
     2521 attatggaaa aaacaggtaa aactgctgaa caagtattgg aaatcttaaa caaagaatca
     2581 ggtttattag gattatcagg tacatctagt gatttacgtg acttatcaga agaagctgaa
     2641 tcaggtaaag cacgttcaca aatggcttta gatgtatttg cttctaaaat tcacaaatat
     2701 attggatcat atgctgcgag aatgcatggt gttgatgtta tcgtatttac agcaggtatc
     2761 ggtgaaaact cagtagaaat tcgtgcgaaa gttcttgaag gcttagaatt tatgggtgtt
     2821 tactgggatc ctaagaaaaa tgaaaactta ctacgtggta aagaaggatt tattaactat
     2881 cctcattcac cagttaaagt tgtcgttatt ccaacagatg aagaaagtat gattgcccgt
     2941 gatgttatga cattcggtgg tttaaaataa tttttaaata aatattaagc gtctatctcc
     3001 gctacaatta tatgtaggag gtagacgctt tttgtattgt ttgaaaatta actagtgtaa
     3061 tgtctctttt cacgaatatc ttaaagaatt aagtagtcga agaagattat gaaaacatta
     3121 ttaagtgagt tttatttata aacaataaaa aaccaagtgc agaaagttgc acttggttca
     3181 taatgatcaa gtagttatct aatgatcatc tatatttaat tttgatattt ttcacgtaat
     3241 tgagttgttg caacttgtgg ttggaagtct gctggtaact cttcagtacg aacaactaac
     3301 acgtcacatg gcgcatgacg aacgatagat tctgatacag aaccaacaat aaatctttcc
     3361 acggcattta agcctgatgt accactcata atcaagtctg cattaatttc atgtgcaagc
     3421 tttttaggaa tgatagattt aggagagcca aactctagac gcgtttctac atctttaaca
     3481 ccagcgttag tagctacttc tttataacca tttaataatt cttctgcaaa atgcttagat
     3541 ttttcagtaa attgagcatc ataaacttca taagaagaat acgttcttga atcaattaca
     3601 ttcacaattg ttaacttcgc atcgttacgt ttagcaacac caactgctct gttaaatgcc
     3661 cattccgctt catgtgaacc gtcaactgcg attaaaatat ttttgtaagt aatcatagta
     3721 agtgcctcct tgttctttct taattatatt ttaccacaat tttcggaaaa gtcagtgtaa
     3781 taacctttac aattttgtgg atttttgatt gcgctttcaa aatgttcgcg ttaagatgat
     3841 tatggaggtg gatggtatga aaattggtat accaagggag attaaaaata atgaaaatcg
     3901 tgttggttta tcaccgagtg gtgtgcacgc tttagtagaa agtgggcata ctgttttagt
     3961 ggaaacaaat gcgggttcag gatcgttctt tgaagatgta gattacaaag aagcaggtgc
     4021 tgagattgtt gctgaacaag caaaagtttg ggatgtggat atggttatta aagttaaaga
     4081 accacttgaa tctgaatatc catattttaa agaagggctt gtattattca cttatcttca
     4141 tttagcaaat gaagaaaaat taacacaagc tttgatagat agaaaagtaa ttagtattgc
     4201 atatgagact gtgcagttac cagaccgatc ttcaccattg ttatcaccaa tgagtgaggt
     4261 agcaggaaga atgtcagctc aagttggcgc agagttccta caaaaactta atggtggtat
     4321 gggaattcta ctaggtggtg tcccaggagt acctaagggt aaagtaacta ttatcggtgg
     4381 tggtcaagca ggaacaaatg cagctaaaat tgcactagga ctaggtgcag atgttacgat
     4441 tttagatgtt aatccaaagc gtttacaaca attagatgat ttattcggtg gacgtgtaca
     4501 tacaattatg tcaaatccgt tgaatattga gttgtatgtt aaacaaagtg atttagtaat
     4561 aggtgcagtt ttaattccag gtgctaaagc gccaagactt gtaacagaag acatgattaa
     4621 acaaatgaaa aatgggtcag ttattattga cattgctatt gatcaaggcg gtatttttga
     4681 aacaactgat aaaattacga cacatgatga tcctacatat attaagcatg gtgtggttca
     4741 ttatgcagtt gcaaatatgc caggtgcagt accgcgtact tcgacgttag ctttaaataa
     4801 tgctacgcta ccttatgcgc tcatgctagc taataaaggg tatagagaag catttaaatc
     4861 aaatcaacca ttatcattag gtttaaatac ttacaaaggt cacgtaacca ataaaggcgt
     4921 tgcagaggca tttgaaatgg aatataaatc tgtagaagaa gcattacaat tataatttga
     4981 atttgatgaa aagggaatgg attacagcgt actaaagaat gacgttatca tctccttggt
     5041 tattaaaatt aaataatgat aaattaaagc tagaaacaat gcgaagaaat catcgaacat
     5101 gaatgatatt gatttgattc attacatgtt actgatgaac atacctgcat atgtgtttct
     5161 agcttgtttg ttacatattg catttagact tggataaaaa tttatgtctt aggcgctaca
     5221 atgttatatt ggcagtgttt gactgaatga gaatgtgctt gtaacaagct tttcattttt
     5281 atcccactcc ttatttttcg taatgtgtta atacttcata tccttcatta gtgacaagta
     5341 tgtcatcttc aattcttaca cctgcaacac ctggtacata aatacctggt tcgattgtaa
     5401 taaccatgcc agcttctaac aaattagaat tagtacttga aacatcttga tattcatgtt
     5461 cttgtaatcc taaaccatga cctaagcgat gagggaaata ttcaccataa cctttttctg
     5521 aaataatatt tctagcgata tgatcgatat cttttaaagg tattccaggt ttaattgctt
     5581 ggattgcaga tgtttctgct tctaatacaa tattataaat ttcttgtgct tctttgctag
     5641 gttcaccaaa tttaatggta cgtgtcatat cgctacaata atgctcataa attacaccta
     5701 aatcaaatag tacatattca ttgcttttta atctgcgatc tcctggtgtg ccatgaggtg
     5761 atgcggcatg atctccaaat aaaaccatcg tatcaaaact catttcattg acgccatatt
     5821 gtttgatagt ttgctcaata tggttgacta cttcacgttc agtcacacct tcttttaaat
     5881 aagaaacacc tatttcgata cacttatctg ctaactcagc agctttacgt attttgctaa
     5941 tttcatcttc ggatttaata tttcttaatt gtttgattgt taaatcaaca tctccgaatg
     6001 aattgacatt gaaaccagag attaattgtt tttggcgtgc tactgttaag tgctcgcttt
     6061 caattagtaa tttattgatt gtttgaggat aaagtgaaaa aggattttca gtgtctaaat
     6121 atccaacgat ttcacctgtg aaaggtgatg ctttgacttc ttcgacttcc atttttggac
     6181 aaaatagtac ttgtttacca tctttcttaa tcaataatgc aaataatctt tcatggggtt
     6241 cgctacggta tccagtaaaa taatatacat tcaacggtgt tgtaatccat gctgcatcag
     6301 cttgttgatt gttcagttcg tctattattt ttgatatttt tgtcatttca taaacactcc
     6361 tcaacataat attacttcca ttttatgcca attattatta gatttcaaaa tcgagctaat
     6421 acattaacta gattttcaat ttttaaaatt tgagaatttg ttataattaa gtggcaaaag
     6481 ggtactaaat ttatatagca attgatagga ggatgctcaa atgaagttat catttcatgg
     6541 tcaatcaacg atttatttag aagggaacaa caaaaaggtt atcgtagatc cgtttatttc
     6601 aaataaccct aagtgtgatt tgaatattga aacagtgcag gtagactata tcgttttaac
     6661 gcacggtcat tttgatcatt ttggtgatgt tgtggaacta gcaaagaaaa caggagcaac
     6721 agtaattgga agtgcagaaa tggctgacta tctttcttca tatcacggtg ttgaaaatgt
     6781 tcatggtatg aatattggtg gtaaagcaaa ctttgatttc ggaagcgtta aatttgttca
     6841 agcatttcat agttcaagtt ttacacatga aaatggtatt ccagtttatt taggtatgcc
     6901 aatgggtatc gtgtttgaag ttgaaggaaa aacaatttat catactggtg atacaggtct
     6961 gtttagtgac atgtcactta ttgcgaaacg tcatccagtt gatgtatgtt ttgtaccaat
     7021 tggagataat tttacgatgg gtattgatga tgcaagttat gctattaatg aatttattaa
     7081 accaaaaatt tctgtaccga ttcattatga tacattccca ttaatcgaac aagatccaca
     7141 acaatttaaa gatgctgtta atgtagggga cgtgcaaatt ttaaaaccag gtgaatctgt
     7201 tcagttttaa atagaatata gattttacac taaaaaacac ctcgctaatc tatgtggaag
     7261 ttatcgtctg catattttta gtgggtgttt ttatattcta ctgattcagt tacgacgtgg
     7321 acgggggaca tcagtggttc tttatcattt ctgacctact ctcgtaattt tagttttata
     7381 caattagaca ttgattataa atttactacg atataaaaaa tccagaacca cagtcataat
     7441 catttcttgt gtttactcag tcgtttgaga ctgcatcatg attgaaagat tatttgtgat
     7501 tctggataaa aattttattt aacgattaat acagggatag tcgcacgttt tgctactttg
     7561 tggctgacac ttccaagtac aaatttcttt ttgtcttctg ctttacggtt acttaaaaca
     7621 acaatttcgt atttaccgct attagcatgt tttaatagtt cttcttttgc attaccacga
     7681 acaattattt ggtcataatc aataccataa tcttctaaag ctttgcgtgt tttttccaat
     7741 cgcttgcttc gttcttcagt aagtttgttt aaatgaacac ctgctttaat tgatgcttga
     7801 gcatcttgtt cgctgattgc gtttaaaact gttacgactg taccttcgcc agctaattta
     7861 gacacttctt ttagtgcttt ttcatttttt aactgagtgt ctacaccaag taatatattt
     7921 ttatacatta gtacatccct cctcacttac attctaataa ataataatgg atttttccat
     7981 ttaaaattaa agattttgtg atctttgtgt ttgttgtata ttttcaaaaa ataaaatagg
     8041 tgaaacgatc attatttcat ttcacttcaa tgctttttat ccaaaataaa gttataatgt
     8101 agttaatact gttcgaaaag aagaggtaaa gaatgacaaa acatgaacaa attttagatt
     8161 atattgaatc gttatctata ggctctaaaa tttcggtgcg taaaatcgct aaatttttaa
     8221 acgtatctga agggacagca tatcgtgcaa ttaaagatgc tgataaaatg ggcatggtcg
     8281 caacgataga tcgtgttggt acggttcgaa tcgaaaaacg taatcgtaat gaaattgaac
     8341 atttaacttt taatgaaatt gtcaatatta ttgatggcca agtgttaggt ggcaataaag
     8401 gtatcactaa gatggtatct aaatttgcaa ttggagcaat ggaactcaaa gatattttaa
     8461 aatatattgg tccaaagaca ttattgattg tcggaaatcg cgaagatgtg cagattgaag
     8521 ctttaaaaag aggtacggct atattaatta ctggtggatt taagccatct aataaagtta
     8581 ttaattttgc gaatgaacat gatttacctg ttctatcatc aagttatgat acattcctag
     8641 ttgcaaatat tatcaacaaa gcacttttta atcagaaaat acgaaaagat atcttaatag
     8701 ttcaagatat aatgacgcca ttagatgatt tgtctgtgct gtttgatacg atgaaaatag
     8761 cagattataa gcgtatggcg aatcgaactg gccatacaag atttccggta gtcaatgagt
     8821 cgtataaatt agtaggtatt gtaacaagtc gagagatgat taatacaaag gacgacgacg
     8881 aaatagataa agtgatgacg cgaaacccta tttatgtaaa tgctatgagt acagtagcaa
     8941 gttgtgcgca tatgatgatt tgggaaggta ttgaattgat tcctgtcgtt tcgagtaata
     9001 aaaagacagt gggtgtgatt aatagacaag atgttttaaa gtctatgcaa cttttaggaa
     9061 gacagcctca gatgggtgaa acaattaacg atcaaatcgc caaatatata acaatgaatc
     9121 aagatggaat tacagtagaa gtttcaccac ttttaatcaa tcattacgga acagtaagta
     9181 aagcggcatt tgtaagtatt atagaagaaa caatacaata tgaaatgcgt aaatttaaaa
     9241 aaggtaatgt catgatagag aacttaaata tcgtgtatat taaaacagta cctattgaat
     9301 cacatataac agtgcgcttt ggcatactag acgtcggaag aaacttcgcc aaaatagaag
     9361 tgaacatgca tagtcaaaac gacaaagtag cgagcgctct cgtgatatgc caaatgtttg
     9421 acgaagtata ggaacttagc agagaaaata ggcataagat agatttcgca caaacgttgg
     9481 agcccaactt acattgtttg tagaatttct tttcgaaatt ctttgtgttg gggccccacc
     9541 ccaacttgca tcgtctgtag aaattggggg tccaatttca ctgtgttggg gcccctgaac
     9601 taaatagtat ggaaatatat atatctaaat aattaaaacg g
//
LOCUS       DANBWH010000101         9539 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.104, whole genome shotgun sequence.
ACCESSION   DANBWH010000101 DANBWH010000000
VERSION     DANBWH010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9539
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.104"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            87..551
                     /locus_tag="PPY92_001669"
     CDS             87..551
                     /locus_tag="PPY92_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDJ2625663.1"
                     /translation="MDRQSFTDLIQTKFKMVRIEAGYTQDTMAQTIGLSKKTLVQIEK
                     ERVLPNWTTCISICALFRDSEVLNSTFGCDPLEIVQTISRNHCAYPNHAPTSDIYWNN
                     IETRNGYILQSNKVSNIYRVLNPDNQPIFGTSKMREAETYFNRNAKEELVHI"
     gene            730..1854
                     /locus_tag="PPY92_001670"
     CDS             730..1854
                     /locus_tag="PPY92_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HDJ2625664.1"
                     /translation="MNALFIIIFMIVVGAIIGGITNVIAIRMLFHPFKPYYIFKFRVP
                     FTPGLIPKRREEIATKIGQVIEEHLLTETLINEKLKSEQSQQAIESMIQQQLQKLTKD
                     QLSIKQITSQIDIDLEQVLQTNGNQYIESQLNNYYTKHQNQTIASLLPNQLVTFLDQH
                     VDNATDLLCDRARNYLSSAKGTQDINDMLDTFFHEKGKLIGMLQMFMTKESIADRIQQ
                     ELIRLTSHPKARTIVTSLITNEYQTFKDKPLNELLDASQFNEIAENLSVYVTTYASNQ
                     ANKPVVTLMPQFVDYLEGQLSSKLANLIIEKLSIHLSTIMKKVDLRGLIEEQINTFDL
                     DYIEKLIIEIANKELKLIMSLGFILGGIIGFFQGLVAIFV"
     gene            1923..2267
                     /locus_tag="PPY92_001671"
     CDS             1923..2267
                     /locus_tag="PPY92_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HDJ2625665.1"
                     /translation="MAVNLYDYANQLEQALRESEEYKAIKEAFANVKANEESKKLFDE
                     FRETQINFQQKQMQGEEIAEEDLQKAQEQAQAIEKDENISALMNAEQKMSQVFQEINQ
                     IIVKPLDEIYAD"
     gene            2806..2904
                     /locus_tag="PPY92_001672"
     CDS             2806..2904
                     /locus_tag="PPY92_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625666.1"
                     /translation="MSVGSLAVLTITEIAFPTEIFEKNQRTLQIKF"
     gene            3195..4391
                     /locus_tag="PPY92_001673"
     CDS             3195..4391
                     /locus_tag="PPY92_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDJ2625667.1"
                     /translation="MVKFIHCSDLHLDSPFKSKSHISPKIFEDVQKSAYESFKNIVDI
                     ALQQDVDFVIIAGDLFDSENRTLRAEIFLKQQFERLQNEQIFVYVCHGNHDPLSSKIS
                     SNWPDNVSVFSNKVETYEAITKSGETIYIHGFSYENRASYENKIDEYPSSQGQKGIHI
                     GVLHGTYSKSSVNERYTEFILEDLNSKLYHYWALGHIHERQQLSDMPVINYSGNIQGR
                     HFNEQGEKGCLLIEGDHLKLKTKFYPTQYIRFEEATIETDKTSKQGLYEVIQNFKEQV
                     REEGKAFYRLTLVINSETLISPQDLLQVEEMITDYEENENQFVYIDELKIQYAQNDES
                     PLVNEFSAELLVDQTVFDKAMSDLYLNPRASKFLDDYGTFDHTALVNRAEEILKAEMR
                     GEQNDN"
     gene            4381..7317
                     /locus_tag="PPY92_001674"
     CDS             4381..7317
                     /locus_tag="PPY92_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDJ2625668.1"
                     /translation="MIIKSLEIYGYGQFVQRKIEFNKNFTEIFGENEAGKSTIQAFIH
                     SILFGFPTKKSKEPRLEPRLGNQYGGKLVLILDDGLEIEVERIKGSAQGDVKVYLPNG
                     AVRDDAWLQKKLNYISKKTYQGIFSFDVLGLQDIHRNLNEKQLQDYLLQAGALGSTEF
                     TSMREVINRKKDELYKKSGKNPIINQQIEQLKQLESQIREEEAKLETYHRLVDDRDKS
                     SRRLENLKHNLNQLSKMHEEKQKEVALHDHSQEWKSLEQQLNIEPITFPEKGVDRYEK
                     ARAHKQSLERDIGLRNERLAQLKEEATQLEPVKQSDIDAFISLNQQENEIKNKEFELT
                     AIEKDIANKQRDKDELQANIGWSETHHDVDSSEAMKSYVSEQIKNKQEQAAYIKQLER
                     SLEENKIEDNAVHSELDSVEEKIVPEETFEKKKEYSQQVIELNEKENLYSKLKERFEI
                     EQQEKQKRQKLLRTTFILLTLVGIGLTAFSFISNNMLFGIIFAVLTLVFVIGIIMSKS
                     KEVDYSEAITDEIEEIKAQLAILDENYDLDFDLDEQYRIRDHWQQALKNKDILEEKRQ
                     YIEGRLNDAKGRHDELQSTVENVKDELYLSSKISNDLIVDSISTMANIKALDQHISDL
                     NQQRQQLVQELDTFYNHAEAVTKSQFVYFNKLSLFHDVQQWLKSAEDTNEKWRINAEN
                     TKLVTNELNHLNAQLEENNKEITALFDFINVGTEEDFYQHHEDYQTYTSNLSRFNDLT
                     KYLENQNYSYELSSSLSEKTTAQLEEEDHLLATQVDEYNEQYLEMQAQVSDLSAQINH
                     METDTTLANLRHEYHSLKNQLNDIAKDWASLSYLQSLVDEHIKQIKDKRLPQVINEAV
                     EILKHLTDGRYTMINYNEDSITVKHVNGQLYDPVELSQSTKELLYVALRISLIKVLRP
                     YYPFPLIVDDAFVHFDKKRTEKMLNYLRSLSEHYQVLYFTCVKDNIVPSKEVITLNKI
                     EEGGKR"
     gene            7314..8255
                     /gene="yhaM"
                     /locus_tag="PPY92_001675"
     CDS             7314..8255
                     /gene="yhaM"
                     /locus_tag="PPY92_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM"
                     /protein_id="HDJ2625669.1"
                     /translation="MRNIENLNPGDSVDHFFLVHKATQGVTAQGKDYMTLHLQDKSGE
                     IEAKFWTATKNDMATIKPEEIVHVKGDIINYRGNKQMKVNQIRLATTEDQLKTEQFVD
                     GAPLSPAEIQEEISHYLLDIENANLQRITRHLLKKYQERFYTYPAASSHHHNFASGLS
                     YHVLTMLRIAKSICDIYPLLNKSLLYSGIILHDIGKVRELSGPVATSYTVEGNLLGHI
                     SIASDEVVEAARELNIEGEEIMLLRHMILSHHGKLEYGSPKLPYLKEAEILCYIDNID
                     ARMNMFEKAYKKTDKGQFTDKIFGLENRRFYNPESLD"
     gene            complement(8376..9338)
                     /locus_tag="PPY92_001676"
     CDS             complement(8376..9338)
                     /locus_tag="PPY92_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HDJ2625670.1"
                     /translation="MKMINKLIVPVTASALLLGACGASATDSKENTLISSKAGDVTVA
                     DTMKKIGKDQIANASFTEMLNKILADKYKNKVNDKKIDEQIEKMQKQYGGKDKFEKAL
                     QQQGLTADKYKENLRTAAYHKELLSDKIKISDSEIKEDSKKASHILIKVKSKKSDKEG
                     LDDKEAKQKAEEIQKEVSKDPSKFGEIAKKESMDTGSAKKDGELGYVLKGQTDKDFEK
                     ALFKLKDGEVSEVVKSSFGYHIIKADKPTDFNSEKQSLKEKLVDQKVQKNPKLLTDAY
                     KDLLKEYDVDFKDRDIKSVVEDKILNPEKLKQGGAQGGQSGMSQ"
BASE COUNT         3540 a         1361 c         1632 g         3006 t
ORIGIN      
        1 gatgtaatct gtgtttcaag aaatgtgtat tattgcaatt tcttgtgtcc ggataaaatt
       61 ccccaaacag aaaggtagat aacagaatgg atagacagag ttttacagat ttaattcaaa
      121 caaaatttaa aatggttcgt atagaggctg gttatacgca agatactatg gcgcaaacaa
      181 ttggactttc taaaaagact ttagtacaaa tcgaaaaaga gagagtatta ccaaactgga
      241 caacttgtat ttcaatttgt gcattattca gagactctga agtattaaac agtacatttg
      301 gctgtgatcc attagaaatc gttcaaacaa tttcaagaaa tcactgtgca tatccaaacc
      361 atgcaccaac aagtgatatt tattggaata acattgagac tcgtaatggg tacattttac
      421 aaagtaacaa agtaagcaat atttatcgtg tattaaatcc agataaccaa ccaattttcg
      481 gtacttctaa aatgagagaa gctgaaacat actttaacag aaatgctaaa gaagaattag
      541 tacatatcta ggcctatata cttttaataa ataataaaag cgatgtaaca cacctgtttc
      601 gagaggtgag gcatcgcttt ttgtatgtaa actaataata aattgagcag aaagtgaaat
      661 gattaatgaa ttaaaaaata aaaatagtgg tataatgaat aactatgttg tcacaaagga
      721 gtcaaaatta tgaatgcact atttattatc atatttatga ttgtagtagg ggcgataatt
      781 gggggcatta ctaatgtaat tgcaattaga atgctgtttc acccctttaa accatactat
      841 atatttaaat ttagagtccc atttacacca ggtttaatac cgaaaagacg cgaagaaatt
      901 gcaactaaaa ttggccaagt gattgaagag catttgctta cagaaacttt aattaatgaa
      961 aaattaaaaa gcgagcaatc acagcaagca atagaatcta tgattcaaca gcagttacaa
     1021 aagttgacga aagatcaatt gtcaataaaa caaattactt ctcaaatcga tattgattta
     1081 gaacaagtat tacaaactaa tggaaatcaa tatattgaat cacaattgaa taattattat
     1141 acaaagcatc aaaaccaaac aatagcatct ttattgccaa atcaacttgt aacattttta
     1201 gatcagcatg tagataacgc aacagactta ttatgtgatc gtgcaaggaa ttatttatca
     1261 tctgcaaaag gcacacaaga tattaatgat atgttggata cttttttcca tgagaaaggt
     1321 aagttaattg gtatgttgca aatgtttatg acaaaagaga gcattgcaga tcgcattcaa
     1381 caagaactta tacgtttaac atctcatcct aaagcaagaa caattgtgac atcgttaatt
     1441 accaatgaat atcaaacttt taaagataaa ccattgaatg aactgttaga tgcatcgcag
     1501 tttaatgaaa tcgctgaaaa tctatcagtg tatgtaacaa catatgcaag caatcaagcg
     1561 aataagccag tggttacgtt aatgcctcaa ttcgttgatt atttagaagg tcaactctct
     1621 agcaaattag caaatttaat cattgaaaaa ttatcgattc acttatctac aattatgaaa
     1681 aaagtggatt tacgaggatt aattgaagaa caaattaata cgtttgactt agattatatt
     1741 gaaaaattaa ttatagaaat tgctaacaaa gaactaaagt tgattatgtc attaggtttc
     1801 attttaggtg gtattattgg atttttccaa ggtttggttg caatctttgt ctaaaaaagc
     1861 gagatgtggt aaagtaatat cgaacgtttt aataaacatc acaacaaagg agtgctataa
     1921 ccatggcagt aaatttatat gattatgcaa atcaattaga acaagcttta agagaaagcg
     1981 aagaatacaa agcaatcaaa gaagcattcg ctaatgtaaa agctaacgaa gaatctaaaa
     2041 agttattcga tgagttccgt gaaactcaaa ttaacttcca acaaaaacaa atgcaaggtg
     2101 aagaaattgc tgaagaagat ttacaaaaag cgcaagaaca agcgcaagca attgaaaaag
     2161 atgaaaacat ctctgcatta atgaatgctg aacaaaaaat gagtcaagta ttccaagaaa
     2221 tcaaccaaat tatcgttaaa ccattagacg aaatttacgc tgactaatat taaaataata
     2281 tttttcaatc ctttaaatag aggaatagac tgccgacaga gtccgagact ttgtgggtag
     2341 tttttttagt tttgataacg gaagttagag gctctctgtc aaattgggca gatgcgttca
     2401 ctatcggaat ttaggctact aaacatgtcc tagctttagc tcagaatgga cgacggaagc
     2461 tgggcaatca aacatggcta gctctatgcc aaaacgggca gatgagcttg ataacggaag
     2521 ttagaggttc tatgtcaaat tgggcagatg cattcagtaa cggaagttag gctagtaaac
     2581 gtgtcctaac ttcctttttg cattttgaag tgtaaagttt tcaacataat actattagtt
     2641 cggtcatgta tcggactgat ggaaaagcgt ttcactttta atgactcatt aagaacggcc
     2701 tgaaaatgtt tggcgtatta agtgcaatga tagttttgac atttagtttc taattggtca
     2761 ttactgccga gcaaatctag tagagtaatc atgtaaatct ttaatgtgtc agttggttca
     2821 ctagcggtgt taacaattac ggaaattgca tttccgactg aaatttttga aaaaaatcaa
     2881 cgcacgctac aaataaaatt ttaaactgtt ataaatgtgt ctcaatttca tatgttcatc
     2941 gacgatatga agcgtattat ggtaaaatga agaaataata aacttgttaa taaataaaac
     3001 atcacgattt gactaaagca ctttattatt gtgtagataa taatttttta acgaaataaa
     3061 aatggcgact ggttttaata aatcagctaa tgaatcacta cacctataag tatgaatata
     3121 gtgattagaa tgctttgtat agttggattt tgcaaaattg atgttagaac ctttaattaa
     3181 ggagatatgg atgtatggtt aaatttattc attgttcgga tttacattta gatagtcctt
     3241 tcaaatctaa gagtcatata agccctaaaa tttttgagga tgttcaaaaa agtgcttatg
     3301 aaagttttaa aaatattgta gatattgcat tacaacaaga tgttgatttt gtaattatcg
     3361 cgggtgattt atttgatagt gaaaacagaa cattacgtgc tgaaattttc ttaaaacagc
     3421 aatttgaacg tttacaaaat gaacaaatct ttgtttatgt ttgtcatggt aatcacgatc
     3481 ctttatcatc aaagatttca tcaaactggc cagataatgt ttctgtattt tcaaataaag
     3541 ttgagacgta tgaagcaatt actaaatctg gtgaaacaat ttatattcac ggatttagtt
     3601 atgaaaatag agcaagttat gagaacaaga ttgatgaata tccatcaagt caaggccaaa
     3661 aaggcataca tattggtgtc ttgcatggta cgtatagtaa atcttcagtt aacgaaagat
     3721 ataccgagtt cattttagaa gatttaaaca gtaaattgta tcattattgg gctttaggtc
     3781 atatacatga acgtcaacaa ttaagtgata tgcctgtaat taactattca ggtaatattc
     3841 aaggtagaca ttttaatgag caaggtgaaa aaggttgctt attaatcgag ggtgaccact
     3901 taaaattaaa gactaaattt tatcctacac agtatattag atttgaagaa gcaactattg
     3961 aaacggataa gacatctaag caaggtttat acgaggtcat tcaaaacttt aaagaacaag
     4021 tgagagaaga aggaaaagcc ttttatcgtt taacgcttgt tattaatagt gagacattaa
     4081 tttcacctca agatttatta caagttgaag aaatgattac agattatgaa gaaaacgaaa
     4141 atcaatttgt atatattgat gagttaaaaa tacaatatgc acaaaatgat gagtcacctt
     4201 tagttaatga attttcagcg gaattattag tcgatcaaac tgtttttgat aaagcgatgt
     4261 cagatttata tttaaatcca agggcatcta agttcctaga cgattatgga acattcgacc
     4321 atacagcatt agttaatcgt gctgaagaaa tattaaaagc tgaaatgaga ggtgaacaaa
     4381 atgataatta aatcacttga aatttatggt tacggtcaat ttgttcaacg taaaattgaa
     4441 tttaataaaa acttcactga aatttttggt gaaaatgaag cgggtaaatc gacgattcaa
     4501 gcattcatcc attcgatatt atttggtttt ccaactaaaa agtctaaaga gccaagacta
     4561 gaaccacgtc taggtaacca atacggtggt aaattagtac ttattcttga tgatggctta
     4621 gagattgaag ttgaacgaat taaaggcagt gctcaaggtg atgtgaaagt atatttacct
     4681 aatggtgctg tgcgtgatga tgcttggtta caaaagaaac ttaattatat ttctaaaaag
     4741 acatatcaag gtatcttttc atttgatgta ctagggcttc aagacattca tagaaatcta
     4801 aatgaaaaac aattgcaaga ttatttatta caagcagggg ctttaggatc aactgaattc
     4861 acgtcaatgc gcgaagtgat taatcgtaaa aaagatgaat tatataaaaa atcaggtaaa
     4921 aatccgatca ttaatcaaca aattgagcaa ttaaaacaac tagaaagtca aattcgtgaa
     4981 gaagaagcaa agctagaaac atatcatcgc ttagtagatg atcgagataa atcatcacgt
     5041 cgattagaga atttaaagca taatttaaat caattatcaa aaatgcatga agaaaaacaa
     5101 aaagaggttg ctttacatga tcattcacaa gaatggaagt ctctagaaca acagttaaat
     5161 attgagccaa tcacattccc agaaaaaggt gtggatcgtt acgaaaaagc gcgagcgcat
     5221 aagcaatcgt tagaaagaga tattggttta agaaatgagc gtttagctca acttaaagaa
     5281 gaagcgactc aattagagcc agttaaacaa tctgatattg acgccttcat tagtttgaat
     5341 caacaagaaa atgaaattaa aaataaagaa tttgaactta ctgcaatcga aaaggatatt
     5401 gcgaataaac aacgtgataa agatgaattg caagcaaata ttggttggtc tgaaacgcat
     5461 catgacgtag atagttcaga ggcaatgaaa agttatgtca gtgagcaaat caagaataaa
     5521 caagaacaag ctgcatacat taaacaatta gaacgtagtt tagaagaaaa taaaatcgaa
     5581 gataatgcgg ttcatagcga actagattct gttgaagaaa aaatagttcc tgaagagact
     5641 tttgaaaaga aaaaagaata ctcacaacaa gtcattgaat taaacgaaaa agagaacttg
     5701 tatagcaaat tgaaagaacg tttcgaaatt gagcaacaag aaaaacaaaa acgtcaaaaa
     5761 ttgttacgaa caacgtttat tcttttaaca ttagtaggta ttggtttaac tgccttctca
     5821 tttatttcaa acaacatgtt gtttggtatt attttcgcag tattaacttt agtatttgtt
     5881 atcggtatca tcatgtctaa atcaaaagaa gtagattata gcgaggccat cacagatgaa
     5941 atcgaagaaa ttaaagcaca actagccatt ttagatgaaa attatgactt agattttgat
     6001 cttgatgagc aatatcgcat tcgtgatcac tggcaacaag ctttgaaaaa taaagatatt
     6061 ttagaagaga aacgtcagta tattgaaggt cgtttaaatg atgcgaaagg gcgtcatgat
     6121 gaattacaat cgacagttga gaacgttaag gatgaattat acttatcatc taaaatttca
     6181 aatgatttaa ttgttgatag tatttctaca atggcgaata ttaaagcatt agatcaacat
     6241 atcagtgatt taaatcaaca acgccagcaa ttggttcaag aattagatac attctacaat
     6301 catgctgaag ctgttacaaa atcacaattt gtttatttca acaaattatc tttattccac
     6361 gatgtgcaac aatggttgaa atcagctgaa gacacgaatg aaaaatggcg tattaatgct
     6421 gaaaatacca aacttgttac aaatgaatta aatcatttaa atgctcaatt agaagaaaat
     6481 aataaagaaa ttacagcact attcgatttt atcaatgttg gtactgaaga agatttttac
     6541 caacatcatg aagattacca aacttatact agtaatttga gtcgttttaa tgatttaact
     6601 aaatatcttg agaaccaaaa ctattcttat gaattaagtt ctagtttaag tgaaaaaaca
     6661 actgcacaac ttgaagaaga agatcattta ttggctactc aagttgacga atataatgag
     6721 caatatcttg aaatgcaagc acaagtcagt gatttaagtg cacaaatcaa tcacatggaa
     6781 actgatacaa cgcttgctaa tttaagacat gaatatcaca gtcttaaaaa tcaacttaat
     6841 gatatcgcaa aagattgggc aagtttaagt tatttacaaa gtttagttga tgaacacatt
     6901 aaacaaatta aagataaacg tttgcctcaa gttattaatg aagcggtaga aatattgaag
     6961 catttaacag atggcagata tacgatgatt aactataatg aagattcaat tacggttaaa
     7021 catgttaatg gtcaattata tgatcctgtt gaactaagtc aatctacaaa agaattactt
     7081 tatgtagctt tacgtatcag tttaattaag gtactaagac catattatcc gttcccatta
     7141 attgttgatg atgcatttgt tcattttgat aaaaagcgta ctgaaaaaat gttgaattat
     7201 ttacgatcat tatcagaaca ctatcaagta ctatacttta catgtgtaaa agataatatt
     7261 gttccatcaa aagaagtgat tacattaaac aaaatagagg aaggcgggaa acgatgagaa
     7321 atatagagaa tctaaatccc ggagattcag ttgatcactt tttcttagtg cataaagcta
     7381 cacagggtgt aacagcacaa ggtaaagatt atatgacatt acatttgcaa gataaaagtg
     7441 gtgaaattga agcgaaattt tggacggcta caaaaaatga tatggcaaca atcaagcctg
     7501 aagaaattgt acatgttaaa ggtgacatca taaactatcg cggaaataaa cagatgaaag
     7561 tcaaccaaat tagactagcg acaactgaag atcaattaaa aacagaacaa tttgtagatg
     7621 gtgcaccttt atcaccggca gaaatacaag aagagatttc tcattatttg ctagatattg
     7681 aaaatgctaa tttacaacgt atcacacgtc atttattgaa aaaatatcaa gaacgatttt
     7741 acacatatcc agctgctagt tctcatcatc ataactttgc gagtggctta agctatcatg
     7801 tattgacgat gttacgtatt gcaaaatcaa tttgtgacat ttatccattg ttaaacaaaa
     7861 gtttgttata tagtggtatt attttgcatg atattggtaa agttagagaa ttgagtggtc
     7921 ctgttgcgac gtcgtataca gtcgaaggta acttattagg acacatctcg attgcgagtg
     7981 atgaagtagt tgaagcagct cgtgaattga acattgaagg agaagaaatc atgttgttaa
     8041 gacatatgat tttatctcat catggtaagt tagagtatgg ttcgccaaaa ctgccatact
     8101 taaaagaagc agaaatttta tgctatatcg ataacatcga tgctagaatg aatatgtttg
     8161 aaaaggctta taaaaaaact gacaagggtc agtttacaga taaaatattt ggtcttgaaa
     8221 atcgtagatt ctacaatcct gaatcactcg attaagaatt aaaagatatc ggacagatga
     8281 gtatactgaa acatgagctc ctctgtccga tttttaatgc gaaaaaaccg agcggccgtg
     8341 gtatgatttt tgaaccacgg tcgctcggtt ttgtgttatt ggctcatgcc ggattgtccg
     8401 ccttgtgcgc caccttgttt aagtttttca gggtttaaga ttttatcttc gacaactgat
     8461 ttaatatcac gatctttaaa gtcaacatcg tattctttta atagatcttt gtatgcatca
     8521 gttaataatt ttggattttt ttgtactttc tgatcgacta atttttcttt caggctttgt
     8581 ttttcactgt taaagtctgt tggtttatca gctttaataa tatgatatcc aaagcttgat
     8641 ttaacaacct ctgatacttc accatcttta agcttaaata gtgctttttc aaaatcttta
     8701 tcagtttgtc ctttaagaac ataacctaat tcgccatctt ttttagctga accagtatcc
     8761 attgattctt ttttagcgat ttcaccaaat ttacttggat cttttgaaac ttctttttga
     8821 atttcttcag ctttttgttt cgcttcttta tcgtctaagc cttctttgtc gcttttctta
     8881 gatttaactt taattaaaat gtgtgaagct ttcttgctgt cttctttaat ttcagaatca
     8941 gagattttaa ttttatctga tagtaattct ttatgataag cagcagtacg taaattttct
     9001 ttatatttat cggctgttaa accttgctgt tgaagggcct tttcaaattt atctttaccg
     9061 ccgtattgct tttgcatttt ttcaatttgt tcgtcaatct tcttatcatt aactttattt
     9121 ttatatttat cagctaaaat tttatttaac atttcagtaa atgatgcatt tgcaatttga
     9181 tctttaccga tttttttcat tgtatctgca actgttacat ctccagcttt agaagaaatt
     9241 aatgtatttt ctttagagtc tgtggcacta gcgccacaag cgcctaataa taaagcacta
     9301 gctgttaccg gaacgattaa tttgtttatc atcttcatag ttgaaactcc tttgtaagtt
     9361 aatcatcgtg aatataacat agttttatat tatcaccaat caataaccca tgacatcaga
     9421 ctagagcatt aggtttaggg gtagaaattg atgaaaataa aaaaacaagg tctatataca
     9481 tttgattgta agctattaca tagcctaaaa acaaataata acaccttgct ataattatt
//
LOCUS       DANBWH010000102         9480 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.105, whole genome shotgun sequence.
ACCESSION   DANBWH010000102 DANBWH010000000
VERSION     DANBWH010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9480
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.105"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..426)
                     /locus_tag="PPY92_001677"
     CDS             complement(<1..426)
                     /locus_tag="PPY92_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HDJ2625671.1"
                     /translation="MFLCKRQIDINARFGLPRIAFMSAVATIIMFLVSYEVMYFLSNT
                     PLSDRHFLIFLLLVFMTYPLHKSIHLLFFLPYRKSFKVHKLTKRKWLIFYNTYVNQPV
                     HKFYFCINLILPLIILSAMFVYLTISFPQYGHYFMFLLAL"
     gene            complement(1161..1526)
                     /locus_tag="PPY92_001678"
     CDS             complement(1161..1526)
                     /locus_tag="PPY92_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDJ2625672.1"
                     /translation="MKASRILFGIGVGVAAGFVVALQGRDDKSVKNNTIDRTAPTGSK
                     SELQREFETIKQSFNDILNYGVQIKNESAEFGSSIGGEIKSLLGNFKSDINPNIERLQ
                     SHIENLQNRGEDIGNEISK"
     gene            complement(1668..2090)
                     /locus_tag="PPY92_001679"
     CDS             complement(1668..2090)
                     /locus_tag="PPY92_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HDJ2625673.1"
                     /translation="MSETIFGKILTGEIPSFKVYEDDYVYAFLDISQVTKGHTLLIPK
                     KASANIFETDEETMKHIGAALPKVANAIKRAFNPDGLNIIQNNGEFADQSVFHIHFHL
                     IPRYENDIDGFGYKWETHEDILDNDAKQQIAEQIQAQF"
     gene            2224..2964
                     /locus_tag="PPY92_001680"
     CDS             2224..2964
                     /locus_tag="PPY92_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDJ2625674.1"
                     /translation="MTVKVEQLTGGYGKRPVIKDINFELNKGEIVGLIGLNGAGKSTT
                     IKHMLGLLTPMEGSLSISDININDDIEAYRRKLSYIPESPVIYEELTLEEHIEMTAMA
                     YDIDRDEAMNRAMPLLKTFRLENELKVFPSHFSKGMKQKVMIICAFIVNPELYIIDEP
                     FLGLDPLGIQSMLDLMVEKKNEGRTVLMSTHILATAERYCDRFIILDEGEVVAFGDLE
                     ALRQQTGLHNQTLDDIYIHVTQGGDVHA"
     gene            2957..4180
                     /locus_tag="PPY92_001681"
     CDS             2957..4180
                     /locus_tag="PPY92_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDJ2625675.1"
                     /translation="MHNQATTLFNKRLHALRKEKNYYNKFIFNGHFMVFLLILLGAFI
                     FGYGEWLKHIPTNINFALIAAVIVALTSIFPMRPLLKEADKIFLLPFEKHMSQFMRHA
                     ILYSYFARILIQLIIVIVMFPLFYNINQHNVAFYICFGFSALIFPYVGLRLRWQWYQS
                     GLKTWQVNLISFIIFALTYYLLLAPKWYIVFVMVALPVLIEFLVKKYKPGFLYPWEKM
                     IAIEHRHHMNYYKFVNMFTDVKHLKESAVRRSYLDILLPVPKGSKFNSNAMYLFLFIR
                     SFIRGRDAFNIIFRLVIIAVLLMVWLSYPLVTGVIGSLFVYIILLQMAQFYSQQAYGL
                     WPQVWPVPEEKVIKGYEQFLYRLMFVICTVFAVTFIIKHMTLFYVVLIFYIVGLLTIR
                     SIIKKLKYQETLLRD"
     gene            complement(4304..4807)
                     /gene="traP"
                     /locus_tag="PPY92_001682"
     CDS             complement(4304..4807)
                     /gene="traP"
                     /locus_tag="PPY92_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein TRAP"
                     /protein_id="HDJ2625676.1"
                     /translation="MKKLYTSYGTYGFLHQIKINNPTHQLFQFSASDTSVIFEETDGE
                     TVLKSPSIYEVIKEIGEFSEHHFYCAIFIPSTEDHAYQLEKKLISVDDNFRNFGGFKS
                     YRLLRPAKGTTYKIYFGFADRHAYEDFKQSDAFNDHFSKDALSHYFGSSGQHSSYFER
                     YLYPIKE"
     gene            4970..5080
                     /locus_tag="PPY92_001683"
     CDS             4970..5080
                     /locus_tag="PPY92_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625677.1"
                     /translation="MIKCQFYDIYLLKIRTNERYDNIDKNDFNLGGLYGA"
     gene            5070..6107
                     /gene="hemE"
                     /locus_tag="PPY92_001684"
     CDS             5070..6107
                     /gene="hemE"
                     /locus_tag="PPY92_001684"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDJ2625678.1"
                     /translation="MVHNKNNTILKMIKGEETSHTPVWFMRQAGRSQPEYRKLKEKYS
                     LFDITHQPELCAYVTHLPVDNYHTDAAILYKDIMTPLKPIGVDVEIKSGIGPVIHNPI
                     KTIQDVEKLSQIDPERDVPYVLDTIKLLTEEKLNVPLIGFTGAPFTLASYMIEGGPSK
                     NYNFTKAMMYRDEATWFALMNHLVDVSVKYVTAQVEAGAELIQIFDSWVGALNVEDYR
                     RYIKPHMIRLISEVKEKHDVPVILFGVGASHLINEWNDLPIDVLGLDWRTSINQAQQL
                     GVTKTLQGNLDPSILLAPWNVIEERLKPILDQGMENGKHIFNLGHGVFPEVQPETLRK
                     VSEFVHTYTQR"
     gene            6165..7088
                     /gene="hemH"
                     /locus_tag="PPY92_001685"
     CDS             6165..7088
                     /gene="hemH"
                     /locus_tag="PPY92_001685"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDJ2625679.1"
                     /translation="MTKKMGLLVMAYGTPYKESDIEPYYTDIRHGKRPSEEELQDLKD
                     RYEFIGGLSPLAGTTDDQADALVSALNKAYADVEFKLYLGLKHISPFIEDAVEQMHND
                     GITEAITVVLAPHYSSFSVGSYDKRADEEAAKYGIQLTHVKHYYEQPKFIEYWTNKVN
                     ETLAQIPEEEHKDTVLVVSAHSLPKGLIEKNNDPYPQELEHTALLIKEQSNIEHIAIG
                     WQSEGNTGTPWLGPDVQDLTRDLYEKHQYKNFIYTPVGFVCEHLEVLYDNDYECKVVC
                     DDIGANYYRPKMPNTHPLFIGAIVDEIKSIF"
     gene            7112..8512
                     /locus_tag="PPY92_001686"
     CDS             7112..8512
                     /locus_tag="PPY92_001686"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HDJ2625680.1"
                     /translation="MTKSVAIIGAGITGLSSAYFLKQQDPNIDVTIFEASNRPGGKIQ
                     SYRKDGYMIELGPESYLGRKTIMTELAKDIGLEQDIVTNTTGQSYIFAKNKLYPIPGG
                     SIMGIPTDIKPFVTTKLISPLGKLRAGLDLIKKPIQMQDGDISVGAFFRARLGNEVLE
                     NLIEPLMGGIYGTDIDKLSLMSTFPNFKEKEEAFGSLIKGMKDEKNKRLKQRQLYPGA
                     PKGQFKQFKHGLSSFIEALEQDVKNKGVTIRYNTSVDDIITSQKQYKIVYSNQQEDVF
                     DGVLVTTPHQVFLNWFGQDPAFDYFKTMDSTTVATVVLAFDEKDIENTYDGTGFVIAR
                     TSDTDITACTWTSKKWPFTTPEGKVLIRAYVGKPGDTVVDDHTDNELVSIVRRDLSQM
                     MTFKGDPEFTIVNRLPKSMPQYHVGHIQQIRQIQAHIKQTYPRLRVTGASFEAVGLPD
                     CITQGKVAAEEVIAEL"
     gene            8835..9389
                     /locus_tag="PPY92_001687"
     CDS             8835..9389
                     /locus_tag="PPY92_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase family protein"
                     /protein_id="HDJ2625681.1"
                     /translation="MTDVIIVHSMHGNSRNHWYQWLEHNLTLEGYDVTLFNFESPEAK
                     TVDQWIEAMTKQINVRKKDTYFVTHGLGSITALKYIEMIDQPIEGFFSIAGFKEDAEN
                     IDLDIDLSNVTIDYDNIKKKVDNFYGLSSKNDKYVSYKETQRLMNALEGNLRIVEDGG
                     HFLEEDGFETFTALQDRMQDYMTR"
BASE COUNT         3338 a         1314 c         1733 g         3095 t
ORIGIN      
        1 caatgccaat aagaacataa aataatgtcc atattgcggg aatgaaattg ttagataaac
       61 gaacattgca gataagataa ttaacggcaa tattaagtta atgcaaaaat aaaatttgtg
      121 tacaggttga ttgacgtagg tattatagaa tataagccat tttcttttag ttaacttatg
      181 aactttaaac gattttctat atggtaagaa aaataataaa tgtatacttt tatgtaatgg
      241 atacgtcata aatacaagta ataaaaagat gagaaaatgt ctatctgata atggcgtatt
      301 agataaaaaa tacattactt cataactaac taaaaacata atgatggttg caactgcact
      361 cataaatgca attctaggca aaccaaatcg tgcattgata tcaatttgtc ttttacataa
      421 aaacatatct tacgcctcat ctcttttcaa tataagttag attataacta actcaggttc
      481 agtaattcaa tagtaacata tcatgttaga agaattgtta tataagaaga aaggggagac
      541 acgtattgtt tgatttgaaa cgcgaaagtg gagattggta aaattggctg attgactagc
      601 gtagaagtag aatgcagaac aaatttctgc gaacacatct tcttgctaat aaagcatcac
      661 ttatgaaaaa ggcgcgtgtg acatgagtat ttgaaaactt gaataatata aaaaatggat
      721 atggcagggc atatgcaaaa gcatcttttg accaactaag ttatacaaat gctaaaaata
      781 tagtgatatg taattataat aaataccaga ttaataattt gtgcaagtaa ggtatatgta
      841 atgtgtatat aaggttcggc tcaatgaaaa tataagtatg ttgtttgtcg ataatgatat
      901 aagtaaagcg taatcaaagt gcaaggataa cacaatgaat cctaagcaat gttttcaatc
      961 aattcataaa tatgatttaa gcgaaaatca ctacaaacga tgtattcctg taatttgtaa
     1021 acataagttc atttgagtta taaaaaagag atgacaagaa atatctaatt tctaatgtca
     1081 tctcgtttat ttggcattta atttaaaaaa ataaatatgc tgttactaat aaaaatagtt
     1141 gtgatcgaga acgtaacgtg ctacttagaa atttcgtttc caatatcctc gccacgattt
     1201 tgtaaatttt cgatgtgtga ctgtaaacgt tcaatattag gattaatgtc agatttgaag
     1261 tttccaagta atgacttaat ttcaccacca attgaactac caaattccgc actttcgttt
     1321 ttaatttgaa caccatagtt taaaatgtca ttaaaacttt gtttaatcgt ttcaaattca
     1381 cgttgtagtt ctgattttga accagtaggg gcagtacgat cgatcgtgtt gttcttgaca
     1441 cttttgtcgt ctcgtccttg aagtgcaact acaaaaccag ctgctacgcc aacaccgata
     1501 ccgaatagaa tgcgtgatgc tttcatttat attctcctca cttttaacat ctgttatctt
     1561 gataactcat tttgtttaaa attgctaacc ttgatgttat cacttcaaac gcaattgtta
     1621 taatattaat catattatac ccgttcgagc ttagattaag catacattta aaattgtgct
     1681 tgaatttgtt cagcaatttg ttgttttgca tcgttatcta aaatgtcttc atgtgtttcc
     1741 cacttataac caaatccatc aatatcattt tcgtatcgag gaattaagtg gaaatgaata
     1801 tgaaatacag attgatctgc aaactcacca ttattttgaa taatgtttaa accatcagga
     1861 ttaaatgcac gcttaatagc atttgctact ttaggtaatg ctgcaccgat atgtttcatt
     1921 gtttcttcat cagtttcaaa gatattagca gaagcttttt taggaattaa taacgtatgt
     1981 cctttagtaa cttgtgatat atctaaaaag gcatagacat aatcgtcttc atatacttta
     2041 aagctaggaa tttctccagt taaaattttg ccgaaaattg tttctgacat gatgactaca
     2101 ctccttttca actagtatta ccattcatta taacattgtc gctaaaaatt cgatattagg
     2161 acatgtgtat ttaatatgag cattttataa tttttggtac aatataagtc atggaggtgc
     2221 cttatgacag ttaaagtaga acagcttaca ggtggatatg gaaaacgccc tgtaattaaa
     2281 gatataaatt ttgaattaaa caaaggcgaa atcgttggac ttattggcct taatggcgct
     2341 ggtaagagta caacgattaa acatatgcta ggattgctta cacctatgga aggatctcta
     2401 tcaatttcag atatcaacat taatgatgac atagaggcat atagaagaaa gttatcttat
     2461 attccggaat caccggttat ttatgaagaa ctcacattag aggaacatat agagatgaca
     2521 gcaatggcat atgatattga tcgtgatgaa gcgatgaatc gagcaatgcc attattaaag
     2581 acattccgtt tagaaaatga attgaaagtt tttccgagtc atttttctaa ggggatgaaa
     2641 caaaaagtca tgattatttg tgcgtttatc gttaatcccg aattgtatat tatagacgaa
     2701 ccattccttg gactagatcc attaggaatt caatccatgt tagatttaat ggttgaaaag
     2761 aaaaacgaag gtagaaccgt tctaatgagt acacatattt tagcaacagc tgaacgttat
     2821 tgtgatcgtt ttatcatact agacgaaggt gaagtcgttg catttggaga tttagaagca
     2881 ttgagacaac aaactggctt acataatcaa acattagacg atatatatat tcatgtgacg
     2941 caaggtggag atgtacatgc ataaccaagc gaccacatta tttaataaaa gattacatgc
     3001 ccttcgtaaa gagaaaaatt attataataa gtttatattt aatggtcatt tcatggtctt
     3061 tttacttatt ttgctgggtg catttatttt tggatatggt gaatggttaa aacatattcc
     3121 aacgaatatc aattttgcat taatagcagc agtgattgtt gcgttgacat ccatttttcc
     3181 aatgcgacca ctcctaaaag aagcggataa aatcttttta ttaccatttg aaaaacacat
     3241 gtctcaattt atgcgccatg cgattttata tagttacttt gcgcgcatat taatccaact
     3301 cattattgtt attgtaatgt tcccgttgtt ttacaatatt aatcaacata atgtggcatt
     3361 ttatatttgt tttggattca gtgcattaat ttttccatat gttggtttac gcttaagatg
     3421 gcaatggtat caatcgggac ttaaaacatg gcaagttaat ttaatatcat ttatcatctt
     3481 tgctctaaca tattacttac tattagctcc aaaatggtac atcgtatttg tgatggttgc
     3541 tttgcctgtg ttaatagaat ttttagttaa aaaatataaa ccaggctttt tgtatccttg
     3601 ggagaaaatg attgctatcg aacatcgaca tcatatgaat tattataaat ttgttaatat
     3661 gttcaccgat gtaaaacatt tgaaagaatc tgcagtcaga agaagttatc tagatatact
     3721 tttaccagtt ccaaagggta gtaaatttaa ctctaatgcg atgtatttat tcttatttat
     3781 tagaagcttt ataagaggga gagacgcatt taatattatc tttaggttag taattattgc
     3841 agttttactt atggtttggt tatcatatcc attagtgaca ggggttattg gaagtctttt
     3901 tgtgtatatt atattgttgc aaatggcaca attttattca caacaagcat atggcttatg
     3961 gcctcaagtg tggcctgtac ctgaagagaa ggtaatcaaa gggtatgaac agtttttata
     4021 tagattaatg tttgttattt gtactgtgtt tgcagtgaca tttattataa aacatatgac
     4081 gttattctat gttgtactca ttttctatat tgtaggttta ttaacgatta gaagtattat
     4141 taaaaagttg aaatatcagg aaacattatt acgagactaa aaaacttcca ttggcatgta
     4201 tgttgtaaag gtgcatgtaa tgttgaacgc caaatgatac ggcgttcaga ttacattagc
     4261 atctatacgt taacagcata accaatggaa gttttcttcg agtctattct tttattgggt
     4321 atagatatct ttcaaaataa cttgaatgtt gtccgcttga accaaagtaa tgacttaatg
     4381 cgtcttttga aaaatggtca ttaaaggcat cagattgctt aaagtcttcg tatgcatgtc
     4441 gatcagcaaa tccgaaataa attttatatg ttgtaccttt agcaggtctt aacaaacgat
     4501 agcttttaaa gccaccaaag tttctgaaat tatcgtctac actaatcagt ttcttttcaa
     4561 gttgatatgc atgatcttct gttgaaggaa tgaagattgc acaatagaaa tgatgttcac
     4621 tgaattcacc aatttcttta ataacttcat atattgaagg tgattttaaa acagtctcac
     4681 catcagtttc ttcaaaaata actgaagtat ctgatgctga aaattggaat agttgatggg
     4741 tcgggttatt gatttttatt tgatgtaaaa atccataagt gccataagat gtatatagtt
     4801 tcttcatatg aaaggtcccc cttatgtttt tatttattat acgatgataa actagtcatt
     4861 accactatta ataattgatt aaaattacta taatgaatct tgcgagatat ttagaagtaa
     4921 tgatacaaaa taatggactt tagaatttaa atttacaata aatagttcta tgattaaatg
     4981 tcagttttat gacatttatt tattgaaaat acgaacgaat gagcgatatg ataatataga
     5041 taagaatgat tttaatttag gaggccttta tggtgcataa taaaaacaat acaattttaa
     5101 aaatgatcaa aggtgaagaa acatcacata cacctgtttg gtttatgcga caagctggcc
     5161 gttcgcaacc agaatatcga aaattgaaag aaaaatattc actattcgat attacacatc
     5221 agccggagtt gtgcgcttat gtaacacatt taccagttga taattatcat acagatgcag
     5281 caattttata caaagatatt atgacaccat taaagccaat tggtgtcgat gtagaaatta
     5341 aatcgggtat tggtccagtg attcataatc ctatcaaaac aattcaagat gttgagaaac
     5401 tttctcaaat agaccccgaa cgagatgtac catatgtatt agatacaatt aaacttttaa
     5461 cagaagaaaa gttaaatgtg ccgctaatag gatttactgg ggcaccattt acattagcgt
     5521 catatatgat tgaaggcgga ccatcgaaaa attacaattt tacaaaagcg atgatgtata
     5581 gagatgaagc aacatggttt gctttaatga atcatttagt tgatgtatct gttaaatatg
     5641 taacagctca agtcgaagca ggtgccgaat tgattcaaat tttcgattca tgggtaggtg
     5701 cattaaatgt cgaggattac agacgttaca ttaaaccaca tatgattcga ttaatcagtg
     5761 aggttaaaga aaaacatgat gtgccggtaa ttttattcgg tgtaggtgcc agtcatttaa
     5821 tcaatgaatg gaatgattta ccgattgatg tattaggcct agattggaga acgtctatta
     5881 atcaggctca acaattaggc gttactaaaa cattacaagg gaatttagat ccatcaattt
     5941 tattagcacc atggaatgtc attgaagaga gattgaaacc aatattagat caaggtatgg
     6001 agaacggtaa acacatcttt aatttaggac acggtgtttt cccagaagtg caaccagaga
     6061 cgttacgtaa agtaagcgaa ttcgtacaca catatacaca aagataatca tagtattaca
     6121 ttgaagcaca tgaaagataa ttcatttaaa ggaaggtatt atcaatgact aaaaaaatgg
     6181 ggttattagt tatggcttat ggcacacctt ataaagaaag tgacatagag ccatattata
     6241 cagatattag acatggtaaa cgtccatctg aagaagaact tcaagatttg aaagatagat
     6301 atgaatttat aggtggttta tcaccattag caggtacaac agatgaccaa gctgatgcac
     6361 tagtttcagc attaaataaa gcatatgcag atgttgaatt taaactatac ttaggattaa
     6421 aacacatttc accatttatc gaagatgcgg ttgaacaaat gcacaatgat ggtattactg
     6481 aagcaatcac ggtagtacta gcaccacatt attcttcatt ttcagtagga tcatatgaca
     6541 aacgtgctga tgaagaagct gcaaaatatg gtattcaact tacacatgtg aaacattatt
     6601 atgaacaacc taaatttatt gaatattgga cgaataaagt caacgaaaca ttagctcaaa
     6661 taccggaaga ggaacataaa gacacggtat tagttgtttc ggcacatagt ttgccaaaag
     6721 gtttaatcga aaagaataat gatccatatc cacaagaact agaacatact gcgcttttaa
     6781 ttaaagaaca atctaatatt gaacatatcg cgattggttg gcaatctgaa ggtaatacag
     6841 gtacaccttg gttagggcca gatgtacaag atttaacacg tgatttatat gaaaaacatc
     6901 agtataaaaa ctttatatat acgccagtag gttttgtatg tgagcattta gaggtgcttt
     6961 atgacaatga ttatgaatgt aaagttgttt gcgatgatat tggtgcgaat tattatcgtc
     7021 caaaaatgcc gaatacacat ccattattta tcggtgcaat tgttgatgaa atcaagtcta
     7081 tattttaatg acgaaagaag cgtgaaacgt tgtgactaaa tcagtggcta ttataggagc
     7141 ggggataaca ggtttatcaa gtgcatattt tttaaaacag caagatccta atattgatgt
     7201 aaccatcttt gaagcatcga atcgtccggg gggaaagatt caatcgtatc gtaaagatgg
     7261 ttatatgatt gaactagggc ctgaatctta tttaggtaga aaaacgatta tgacagaatt
     7321 agcgaaagat attggattag aacaagatat tgttacaaat acgactggac aatcatatat
     7381 ttttgcgaaa aataaattat atccgattcc aggtggttca attatgggta ttccaacaga
     7441 tattaaacca tttgttacta caaaattaat atcgccactt ggtaaattaa gagcaggatt
     7501 agatttaatc aaaaagccta tacaaatgca agatggtgac atttctgttg gtgcattttt
     7561 cagagcaaga ttaggtaatg aggtacttga gaacttaatt gagcctttaa tgggtggtat
     7621 ttatggtacc gatattgata aattaagttt gatgagtacg tttcctaatt ttaaagaaaa
     7681 agaagaggca ttcggaagtc tgataaaagg tatgaaggat gagaaaaata agcgtctgaa
     7741 acaaagacaa ttatatcctg gcgcaccaaa aggacaattc aaacaattta agcatggttt
     7801 aagctcattt attgaagcat tagaacaaga tgtgaaaaat aaaggtgtga caatacgcta
     7861 caatacgtca gtggatgata ttattacatc tcaaaagcaa tataaaattg tttacagtaa
     7921 tcaacaagaa gatgtattcg atggggtatt agtgacaaca ccgcatcaag tctttttgaa
     7981 ttggttcgga caagatccag catttgatta ctttaaaacg atggatagta cgactgttgc
     8041 aactgttgta ttggcatttg atgaaaaaga cattgaaaat acttatgatg gtactggctt
     8101 cgtgattgcg agaacgagtg atacagacat taccgcatgt acttggacat cgaaaaaatg
     8161 gccatttact acaccagaag gtaaggtttt gattcgtgcg tatgtaggta aaccaggtga
     8221 tactgtggtt gatgatcata cagataatga attagtatcg attgtacgta gagatttaag
     8281 tcaaatgatg acatttaaag gtgatcctga atttacaatt gtcaatcgtt tgccgaaaag
     8341 tatgccacag taccatgtcg gtcatattca acaaattaga cagattcaag cacatattaa
     8401 acaaacatat ccacgactta gagtaactgg tgcatctttt gaagcggttg gactacctga
     8461 ttgtattaca caaggtaaag ttgctgctga agaagtaatc gcagagttgt aagttaattg
     8521 ttggatggtg ttggtgtagg tgcgacgcct ttagataatg tttgatgaaa tatagtcatt
     8581 tttaagttat ataatggtgg aagtaatgtg taaatataga tactttcagc gggaattatt
     8641 tttgtgaaat tgaagcagag acaaaggttt agctgttttt gaatgatatt gaaatgatga
     8701 caatacactt taattagaaa tgtcactttg cgatataaca tttctaatta aggtgttttt
     8761 attttacaat tataggtaat cagtcatttt tttaaaaata gaaaagaagt agtaagttga
     8821 aaggagagaa tgagatgaca gatgtaatta ttgtacattc tatgcatgga aatagtagaa
     8881 atcattggta tcagtggttg gagcataact taactttaga aggatatgac gtaacattat
     8941 ttaattttga atcccctgaa gcgaagacag tggatcagtg gattgaagct atgacgaaac
     9001 aaattaatgt acgcaaaaag gatacatatt ttgtaacaca tggtttaggt tcaatcacag
     9061 ctttaaaata tattgaaatg attgatcagc cgattgaagg cttttttagt atagcgggct
     9121 ttaaagaaga tgcagaaaat atagatttag atattgattt aagtaatgta accattgatt
     9181 acgataatat taaaaagaaa gttgacaatt tttacggatt gagttctaaa aatgacaaat
     9241 acgtttcata taaagaaacc cagcgtttaa tgaatgcatt agaggggaac ttgcgaatcg
     9301 tggaagatgg agggcacttc ttagaagagg atggcttcga aacatttaca gcgttgcaag
     9361 atcggatgca agattacatg acacgatagg taatgtttgg ttgctatgta ttgtgttatg
     9421 tgtgtcagta cgaagcatag cactttagtg aaaaagatta taattatatt ttaatagttt
//
LOCUS       DANBWH010000103         9456 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.106, whole genome shotgun sequence.
ACCESSION   DANBWH010000103 DANBWH010000000
VERSION     DANBWH010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9456
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.106"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            12..323
                     /locus_tag="PPY92_001688"
     CDS             12..323
                     /locus_tag="PPY92_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDJ2625682.1"
                     /translation="MKQLESEQQFESLKQGATVFEFTAGWCPDCRVIEPDLPELEARY
                     PMFDFVSVDRDKFMDICIENGIMGIPSFLVYKNGELLGSYIGKERKSIEQIDAFLAQY
                     V"
     gene            423..1280
                     /locus_tag="PPY92_001689"
     CDS             423..1280
                     /locus_tag="PPY92_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1444 domain-containing protein"
                     /protein_id="HDJ2625683.1"
                     /translation="MNTFQMRDKLKERLSHLDVDFKFNREEETLRIYRTDNNKGITIK
                     LNAIVAKYEDKKEKIVDEIVYYVDEAIAQMADKTLESISSSQIMPVIRATSFDKKTKQ
                     GVPFIYDEHTAETAVYYAVDLGKSYRLIDESMLEDLKLTEQQIREMSLFNVRKLSNSY
                     TTDEVKGNIFYFINSNDGYDASRILNTAFLNEIEAQCQGEMLVAVPHQDVLIIADIRN
                     KTGYDVMAHLTMEFFTKGLVPITSLSFGYKQGHLEPIFILGKNNKQKRDPNVIQRLEA
                     NRRKFNKDK"
     gene            1309..1905
                     /locus_tag="PPY92_001690"
     CDS             1309..1905
                     /locus_tag="PPY92_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HDJ2625684.1"
                     /translation="MNLFYNPKYVGDVAFLQIEPVEGELNYNKKGNVVEITNEGNVVG
                     YNIFEISKDITIEEKGHIKLTDELVNVFQKRISEAGFDYKLNADLSPKFVVGYVETKD
                     KHPDADKLSVLNVNVGNDTLQIVCGAPNVEAGQKVVVAKVGAVMPSGMVIKDAELRGV
                     ASSGMICSMKELNLPNAPEEKGIMVLNDSYEIGQAFFE"
     gene            1926..5750
                     /locus_tag="PPY92_001691"
     CDS             1926..5750
                     /locus_tag="PPY92_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDJ2625685.1"
                     /translation="MSWFDKLFGEDNDSNDDLIHRKKKRRQESQNIDNDHDSLLPQNN
                     DIYSRPRGKFRFPMSVAYENENVEQSADTISDEKEQYHRDYRKQSHDSRSQKRHRRRR
                     NQTTEEQNYSEQRGNSKISQQSIKYKDHSHYHTNKPGTYVSAINGIEKETHKSKTHNM
                     YSNNTNHRAKDSTPDYHKESFKTSEVPSAIFGTMKPKKLENGRIPVSKPSEKVESDKQ
                     KYDKYVAKTQTSQNKQLEQEKQNDSVVKQGTASKSSDENVSSTTKSMPNYSKVDNTIK
                     IENIYASQIVEEIRRERERKVLQKRRFKKALQQKREEHKNEEQDAIQRAIDEMYAKQA
                     ERYVGDSSLNDDSDLTDNSTDASQLHTNGIENETVSNDENKQASIQNEDTNDTHVDES
                     PYNYEEVSLNQVSTTKQLSDDEVTVSNVTSQHQSALQHNVEVNDKDELKNQSRLIADS
                     EEDGATNKEEYSGSQIDDAEFYELNDTEVDEDTTSNIEDNTNRNASEMHVDAPKTQEH
                     AVTESQVNNIDKTVDNEIELAPRHKKDDQTNLNVNSLKTNDVNDGHVVEDSSMNEIEK
                     HNAEITENVQNEAAESEQNVEEKTIENVNPKKQTEKVSTLSKRPFNVVMTPSDKKRMM
                     DRKKHSKVNVPELKPVQSKQAVSESKPASQAAPSSRSDSQESNTNAYKTNNMTSNNVE
                     NNQLIGHAETENDYQNAQQYSEQKPSADSTQTEIFEESQDDNQLENEQVDQSTSSSVS
                     EVSDITEESEETTHPNNTSGQQDNDDQQKDLQPSFSNQNEDTANENRPRTNQQDVATN
                     QAVQTSKPMIRKGPNIKLPSVSLLEEPQVIESDEDWITDKKKELNDALFYFNVPAEVQ
                     DVTEGPSVTRFELSVEKGVKVSRITALQDDIKMALAAKDIRIEAPIPGTSRVGIEVPN
                     QNPTTVNLRSIIESPSFKNAESKLTVAMGYRINNEPLLMDIAKTPHALIAGATGSGKS
                     VCINSILMSLLYKNHPEELRLLLIDPKMVELAPYNGLPHLVAPVITDVKAATQSLKWA
                     VEEMERRYKLFAHYHVRNITAFNKKAPYDERMPKIVIVIDELADLMMMAPQEVEQSIA
                     RIAQKARACGIHMLVATQRPSVNVITGLIKANIPTRIAFMVSSSVDSRTILDSGGAER
                     LLGYGDMLYLGSGMNKPIRVQGTFVSDDEIDDVVDFIKQQREPDYLFEEKELLKKTQT
                     QSQDELFDDVCAFMVNEGHISTSLIQRHFQIGYNRAARIIDQLEQLGYVSSANGSKPR
                     DVYVTEADLNKE"
     gene            5774..7087
                     /locus_tag="PPY92_001692"
     CDS             5774..7087
                     /locus_tag="PPY92_001692"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDJ2625686.1"
                     /translation="MTHYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIENYVFTEVALR
                     NKGIKILPFDANNIKEDMVVIQGNAFASSHEEIVRAHQLKLDVVSYNDFLGQIIDQYT
                     SVAVTGAHGKTSTTGLLSHVMNGDKKTSFLIGDGTGMGLPESDYFAFEACEYRRHFLS
                     YKPDYAIMTNIDFDHPDYFKDINDVFDAFQEMAHNVKKGIIAWGDDEHLRKIEADVPI
                     YYYGFKDSDDIYAQNIQITDKGTAFDVYVDGEFYDHFLSPQYGDHTVLNALAVIAISY
                     LEKLDVTNIKEALETFGGVKRRFNETTIANQVIVDDYAHHPREISATIETARKKYPHK
                     EVVAVFQPHTFSRTQAFLNEFAESLSKADRVFLCEIFGSIRENTGALTIQDLIDKIEG
                     ASLINEDSINVLEQFDNAVVLFMGAGDIQKLQNAYLDKLGMKNAF"
     gene            7161..7652
                     /locus_tag="PPY92_001693"
     CDS             7161..7652
                     /locus_tag="PPY92_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDJ2625687.1"
                     /translation="MDWILPIAGIIAAIAFLILCIGIVAVLNSVKKNLDYVAKTLDGV
                     EGQVQGITRETTDLLHKVNRLTEDIQGKVDRLNSVVDAVKGIGDSVQTLNSSVDRVTN
                     SITHNISQNEDKISQVVQWSNVAMEIADKWQNRHYRRGSANYKANNVATDANHSYTSR
                     VDK"
     gene            7726..9084
                     /locus_tag="PPY92_001694"
     CDS             7726..9084
                     /locus_tag="PPY92_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625688.1"
                     /translation="MTQQQNDKRTLKNKHTYQNEPLPNRKDFVVSFITGALVGSALGL
                     YFKNKVYQKADDLKVKEQELSQKFEERKTQLEETVAYTKERVEGFLNKSKNEQAALKA
                     QQAAIKEEASANNLSDTSQEAQEIQEAKREAQAEADKSVAVSNEESKASALKVQQAAI
                     KEEASANNLSDTSQEAQEIQEAKKEAQAETDKSAAVSNEEPKAVALKAQQAAIKEEAS
                     ANNLSDTSQEAQEVQEAKKEAQAEKDSDTSTKDASAAKVEVSKPESQAERLANAAKQK
                     QAKLTPGSKESQLTEALFAEKPVAKNDLKEIPQLVTKKNDVSETETVNIDNKDTVKQK
                     EAKFENGVITRKADEKTTNNTAVDKKSGKQSKKTTPSNKRNASKASTNKTSGQKKQHN
                     KKSSQGAKKQSSSSKLTQKNNQTSNKNSKTTNAKSSNASKTPNAKVEKANSKIEKRTF
                     ND"
     gene            9240..9323
                     /locus_tag="PPY92_001695"
     CDS             9240..9323
                     /locus_tag="PPY92_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625689.1"
                     /translation="MLFETSLDNNSYIAHFKLKMEKYSIIK"
BASE COUNT         3764 a         1317 c         1752 g         2623 t
ORIGIN      
        1 gagacataaa aatgaaacaa cttgaatcag aacaacaatt tgaatcttta aaacaaggtg
       61 ctacagtatt tgaattcact gcaggctggt gtccagattg tagagtgata gaaccagatt
      121 taccggaatt agaagcgaga tatcctatgt ttgacttcgt atcagtagac cgtgacaaat
      181 ttatggatat ttgtattgaa aatggtatta tggggattcc aagttttcta gtatataaaa
      241 atggtgaact gcttggaagt tatattggaa aagaacgaaa atcaattgaa cagatagatg
      301 catttttagc tcaatatgtg taatttagac tagagaaaaa cggggtaata ctcgtttttc
      361 tctgttacta tgtgttgatt tattgtaaac tattataagg tgcgaaatta ggagtgttac
      421 atatgaatac ctttcaaatg agagataaat taaaggaacg tttaagccat ttagacgttg
      481 attttaaatt taatcgtgaa gaagaaactt tgcgtattta tcgaacagat aataacaaag
      541 gtatcacgat taaacttaac gctatagtcg caaaatatga agataaaaaa gaaaaaattg
      601 tagatgaaat tgtttattac gttgatgaag ctattgcaca aatggcagat aaaacccttg
      661 agagtatatc atctagtcaa attatgcctg tcattagagc gactagcttt gataaaaaaa
      721 ctaaacaagg tgttcctttt atctatgatg agcatactgc agaaacagca gtttattatg
      781 cagtcgattt agggaaatca tatcgtctaa ttgacgaaag catgttagag gatttgaagt
      841 taactgaaca acaaataaga gaaatgtctc tgtttaatgt tagaaaattg tcaaattcat
      901 atacgactga tgaagtaaaa ggtaatattt tttattttat taactcaaat gacgggtatg
      961 atgcaagtag gatactaaat actgcatttt taaatgaaat tgaggcacaa tgtcaaggcg
     1021 aaatgctcgt agcagtgcca caccaagatg tgttaattat tgcagatata cgcaataaaa
     1081 caggatatga tgtgatggca catttaacaa tggaattttt cactaaaggt ctagttccaa
     1141 ttacatcatt atcctttgga tataaacagg gtcatcttga accgatattt attttaggta
     1201 aaaataataa acaaaaaaga gatccaaacg tgattcagcg tttagaagca aatcgtcgta
     1261 aatttaataa agataaatag aaataattgg ataaggagtt ttgtcataat gaatttattt
     1321 tacaatccta aatatgtagg agatgtcgca tttttacaaa ttgaaccagt tgaaggtgaa
     1381 ttaaactaca ataaaaaagg taatgttgtt gaaattacta atgaaggtaa tgttgtaggt
     1441 tataatattt ttgaaatttc aaaagatata acaattgaag aaaaaggtca tattaaatta
     1501 actgatgaac ttgtaaatgt attccaaaag cgtatttcag aagctggttt tgattataaa
     1561 ttaaatgctg atctatcacc gaaatttgta gttggctacg ttgaaactaa agacaaacat
     1621 cctgatgcag ataaattaag tgtactaaat gtaaacgttg gaaatgacac attacaaatt
     1681 gtatgtggcg cgcctaacgt tgaagctgga cagaaagttg ttgttgctaa agtaggtgca
     1741 gtgatgccta gcggtatggt aattaaagat gctgaattac gtggtgttgc ctcaagcggt
     1801 atgatttgtt caatgaaaga attgaattta cctaatgcac ctgaagaaaa aggtattatg
     1861 gtattaaatg acagctatga aattggacaa gcattttttg aataattaag gaaggtagtg
     1921 aaaatatgag ctggtttgat aaattattcg gcgaagataa tgattcaaat gatgacttga
     1981 ttcatagaaa gaaaaaaaga cgtcaagaat cacaaaatat agataacgat catgactcat
     2041 tactgcctca aaataatgat atttatagtc gtccgagggg aaaattccgt tttcctatga
     2101 gcgtagctta tgaaaatgaa aatgttgaac aatctgcaga tactatttca gatgaaaaag
     2161 aacaatacca tcgagactat cgcaaacaaa gccacgattc tcgttcacaa aaacgacatc
     2221 gccgtagaag aaatcaaaca actgaagaac aaaattatag tgaacaacgt gggaattcta
     2281 aaatatcaca gcaaagtata aaatataaag atcattcaca ttaccatacg aataagccag
     2341 gtacatatgt ttctgcaatt aatggtattg agaaggaaac gcacaagtca aaaacacata
     2401 atatgtattc taataataca aatcatcgtg ctaaagattc aactccagat tatcacaaag
     2461 aaagtttcaa gacttcagag gtaccgtcag ctatttttgg cacaatgaaa cctaaaaagt
     2521 tagaaaatgg tcgtatccct gtaagtaaac cttcagaaaa agttgagtca gataaacaaa
     2581 aatatgataa atatgtagct aagacgcaaa cgtctcaaaa taaacaatta gaacaagaaa
     2641 aacaaaatga tagtgttgtc aaacaaggaa ctgcatctaa atcatctgat gaaaatgtat
     2701 catcaacaac aaaatcaatg cctaattatt caaaagttga taatactatc aaaattgaaa
     2761 atatttatgc ttcacaaatt gttgaagaaa ttagacgtga acgagaacgt aaagtgcttc
     2821 aaaagcgtcg atttaaaaaa gcgttgcaac aaaagcgtga agaacataaa aacgaagagc
     2881 aagatgcaat acaacgtgca attgatgaaa tgtatgctaa acaagcggaa cgctatgttg
     2941 gtgatagttc attaaatgat gatagtgact taacagataa tagtacagat gctagtcagc
     3001 ttcatacaaa tggcatagag aatgaaactg tatcaaatga tgaaaataaa caagcgtcaa
     3061 tacaaaatga agacactaat gacactcatg tagatgaaag tccatacaat tatgaggaag
     3121 ttagtttgaa tcaagtatcg acaacaaaac aattgtcaga tgatgaagtt acggtttcga
     3181 atgtaacgtc tcaacatcaa tcagcactac aacataacgt tgaagtaaat gataaagatg
     3241 aactaaaaaa tcaatccaga ttaattgctg attcagaaga agatggagca acgaataaag
     3301 aagaatattc aggaagtcaa atcgatgatg cagaatttta tgaattaaat gatacagaag
     3361 tagatgagga tactacttca aatatcgaag ataataccaa tagaaacgcg tctgaaatgc
     3421 atgtagacgc tcctaaaacg caagagcacg cagtaactga atctcaagtt aataatatcg
     3481 ataaaacggt tgataatgaa attgaattag caccgcgtca taaaaaagat gaccaaacaa
     3541 acttaaatgt caactcattg aaaacgaatg atgtgaatga tggtcatgtt gtggaagatt
     3601 caagcatgaa tgaaatagaa aagcataacg cagaaattac agaaaatgtg caaaacgaag
     3661 cagctgaaag tgaacaaaat gtcgaagaga aaactattga aaacgtaaat ccaaagaaac
     3721 agactgaaaa ggtttcaact ttaagtaaaa gaccatttaa tgttgtcatg acgccatctg
     3781 ataaaaagcg tatgatggat cgtaaaaagc attcaaaagt caatgtgcct gaattaaagc
     3841 ctgtacaaag taaacaagct gtgagtgaaa gcaagcctgc gagtcaagcc gcaccatcat
     3901 caagatctga ttcacaagag tcaaacacaa atgcatataa aacaaataat atgacatcaa
     3961 acaatgttga gaacaatcaa cttattggtc atgcagaaac agaaaatgat tatcaaaatg
     4021 cacaacaata ttcagagcag aaaccttctg ctgattcaac tcaaacggaa atatttgaag
     4081 aaagccaaga tgataatcaa ttggaaaatg agcaagttga tcaatcaact tcgtcttcag
     4141 tttcagaagt aagcgacata actgaagaaa gcgaagaaac aacacatcca aacaatacta
     4201 gtggacaaca agataatgat gatcaacaaa aagatttaca gccttcattt tcaaatcaaa
     4261 atgaagatac agctaatgaa aatagacctc ggacgaacca acaagatgtt gcaacaaatc
     4321 aagctgtaca aacatctaag ccgatgattc gtaaaggccc aaatattaaa ttgccaagtg
     4381 tttcattact agaagaacca caagttattg agtcggacga ggactggatt acagataaaa
     4441 agaaagaact gaatgacgca ttattttact ttaatgtacc tgcagaagta caagatgtaa
     4501 ctgaaggtcc aagtgttaca agatttgaat tatcagttga aaaaggtgtt aaagtttcaa
     4561 gaattacggc attacaagat gacattaaaa tggcattggc agcgaaagat attcgtatag
     4621 aagcgcctat tccaggaact agtcgtgttg gtattgaagt tccgaaccaa aatccaacga
     4681 cagtcaactt acgttctatt attgaatctc caagttttaa aaatgctgaa tctaaattaa
     4741 cagttgcgat ggggtataga attaataatg aaccattact tatggatatt gctaaaacgc
     4801 cacacgcact aattgcaggt gcaactggat cagggaaatc agtttgtatc aatagtattt
     4861 tgatgtcttt actatataaa aatcaccctg aggaattaag attattactt atcgatccaa
     4921 aaatggttga attagctcct tataatggtt tgccacattt agttgcaccg gtaattacag
     4981 atgtcaaagc agctacacag agtttaaaat gggccgtaga agaaatggaa cgacgttata
     5041 agttatttgc acattaccat gtacgtaata taacagcatt taacaaaaaa gcaccatatg
     5101 atgaaagaat gccaaaaatt gtcattgtaa ttgatgagtt ggctgattta atgatgatgg
     5161 ctccgcaaga agttgagcag tctattgcta gaattgctca aaaagcaaga gcatgtggta
     5221 ttcatatgtt agtagctacg caaagaccat ctgtcaatgt aattacaggt ttaattaaag
     5281 ccaacatacc aacaagaatt gcatttatgg tatcatcaag tgtagattcg agaacgatat
     5341 tagacagtgg tggagcagaa cgcttgttag gatatggcga tatgttatat cttggtagcg
     5401 gtatgaataa accgattaga gttcaaggta catttgtttc tgatgacgaa attgatgatg
     5461 ttgttgattt tatcaaacaa caaagagaac cggactatct atttgaagaa aaagaattgt
     5521 tgaaaaaaac acaaacacaa tcacaagatg aattatttga tgatgtttgt gcatttatgg
     5581 ttaatgaagg acatatttca acatcattaa tccaaagaca tttccaaatt ggctataata
     5641 gagcagcaag aattatcgat caattagagc aactcggtta tgtttcgagt gctaatggtt
     5701 caaaaccaag ggatgtttat gttacggaag cagatttaaa taaagaataa ttatgagtaa
     5761 ggagttttat ataatgacac actatcattt tgtcggaatt aaaggttctg gcatgagttc
     5821 attagcacaa atcatgcatg atttaggaca tgaagttcaa ggatcggata ttgagaacta
     5881 cgtatttaca gaagttgctc ttagaaataa ggggataaaa atattaccat ttgatgctaa
     5941 taacataaaa gaagatatgg tagttataca aggtaatgca ttcgcgagta gccatgaaga
     6001 aatagtacgt gcacatcaat tgaaattaga tgttgtaagt tataatgatt ttttaggaca
     6061 gattattgat caatatactt cagtagctgt aactggtgca catggtaaaa cttctacaac
     6121 aggtttatta tcacatgtta tgaatggtga taaaaagact tcatttttaa ttggtgatgg
     6181 cacaggtatg ggattgcctg aaagtgatta tttcgctttt gaggcatgtg aatatagacg
     6241 tcacttttta agttataaac ctgattacgc aattatgaca aatattgatt tcgatcatcc
     6301 tgattatttt aaagatatta atgatgtttt tgatgcattc caagaaatgg cacataatgt
     6361 taaaaaaggt attattgctt ggggtgatga tgaacatcta cgtaaaattg aagcagatgt
     6421 tccaatttat tattatggat ttaaagattc ggatgacatt tatgctcaaa atattcaaat
     6481 tacggataaa ggtactgctt ttgatgtgta tgtggatggt gagttttatg atcacttcct
     6541 gtctccacaa tatggtgacc atacagtttt aaatgcatta gctgtaattg cgattagtta
     6601 tttagagaag ctagatgtta caaatattaa agaagcatta gaaacgtttg gtggtgttaa
     6661 acgtcgtttc aatgaaacta caattgcaaa tcaagttatt gtagatgatt atgcacacca
     6721 tccaagagaa attagtgcta caattgaaac agcacgaaag aaatatccac ataaagaagt
     6781 tgttgcagta tttcaaccac acactttctc tagaacacaa gcatttttaa atgaatttgc
     6841 agaaagttta agtaaagcag atcgtgtatt cttatgtgaa atttttggat caattagaga
     6901 aaatactggc gcattaacga tacaagattt aattgataaa attgaaggtg catcgttaat
     6961 taatgaagat tctattaatg tattagaaca atttgataat gctgttgttt tatttatggg
     7021 tgcaggtgat attcaaaaat tacaaaatgc atatttagat aaattaggca tgaaaaatgc
     7081 gttttaatat gtttataata gagtagtatg ggtatttatt attaatgaca ttattacatg
     7141 ttaattagga ggcgttttta atggattgga ttttaccaat tgctggaatt atcgctgcga
     7201 ttgcattctt aattttatgt atcggtatcg tagctgtatt aaattctgtt aagaaaaact
     7261 tagattatgt tgcaaaaaca cttgacggtg tagaaggtca agttcaaggt attactcgtg
     7321 aaacaacaga tttacttcat aaagtaaacc gtttaactga ggatatccaa ggtaaagtag
     7381 atcgtttaaa ctcagttgta gatgctgtta aaggtatcgg tgactcagta caaacgttaa
     7441 acagctctgt agatcgtgta acaaattcaa ttacacataa tatttctcaa aatgaagata
     7501 aaatctcaca agttgttcaa tggtcaaatg ttgcaatgga aattgcagac aaatggcaaa
     7561 atagacacta ccgtcgtgga agtgcaaatt acaaagctaa taatgtagca actgatgcaa
     7621 atcatagcta tacttctaga gtagataaat aattaataat taaacaaatg cacttaaatg
     7681 aggttgttac ttatttaagt gcatttgtta taggaggata tacaaatgac gcagcaacaa
     7741 aatgataaaa gaacattaaa aaataaacac acttatcaaa atgaaccact accaaaccgt
     7801 aaagattttg ttgttagttt tataactggc gcgcttgttg gttcagcttt aggtttgtat
     7861 tttaaaaata aagtttatca aaaagcagat gatttaaaag tcaaagaaca agaactgtcg
     7921 caaaagtttg aagaaagaaa aacgcaactt gaagaaacgg ttgcctatac aaaagaacgt
     7981 gttgaaggat ttttaaacaa atctaaaaat gagcaagcgg cattaaaggc acaacaagca
     8041 gcgataaaag aagaagcaag tgcaaataat ttaagtgata catcacaaga agcacaagag
     8101 attcaagaag ctaaaagaga agcacaagca gaagcggata aaagtgtggc tgtatcaaat
     8161 gaagagtcaa aagcatcggc attaaaagta caacaagcag caataaaaga agaagcaagt
     8221 gcaaataatt taagtgacac gtcacaagag gcacaagaga ttcaagaagc taaaaaagaa
     8281 gcacaagcag aaacagataa aagtgcagct gtatcaaatg aagaaccaaa agcagtggca
     8341 ttgaaagcac aacaagcagc gataaaagaa gaagcaagtg caaataattt aagtgataca
     8401 tcacaagagg cacaagaggt tcaagaagct aaaaaagaag cacaagcaga gaaagacagt
     8461 gacacatcaa ctaaagatgc aagtgcagca aaggtagaag tatcaaaacc agagtcacaa
     8521 gctgaaagat tagcaaacgc tgcaaaacag aagcaagcta aattaacacc aggttcaaaa
     8581 gagagtcaat taactgaagc gttatttgca gaaaaaccag ttgctaaaaa tgacttgaaa
     8641 gaaattcctc aattagttac taaaaagaat gatgtatcag agacagagac ggttaatata
     8701 gataataaag acactgttaa acaaaaagaa gctaaatttg aaaatggtgt tattacacgt
     8761 aaagctgatg aaaaaacaac taataataca gctgttgaca agaaatcagg taaacaatct
     8821 aaaaaaacaa caccttcaaa taaacgaaat gcatcaaaag catctacaaa taaaacttca
     8881 ggtcagaaaa agcaacataa taagaaatca tcacaaggtg caaagaaaca aagtagttca
     8941 agtaagttaa ctcaaaagaa taatcaaact agtaataaga attcaaaaac aacaaatgct
     9001 aaatcatcca atgcatcaaa aacgccaaat gctaaagttg aaaaagctaa tagtaaaata
     9061 gagaaacgta cattcaatga ctaagattac taaaatcgtt gaataatcaa tcatagcagt
     9121 aagctatgta ttatattttt aaaaattgaa tttcaaaata aaaggtaggc attcaggtgc
     9181 ttaccttttt tgatatcatc agtttatttt gaaaggcaac gcgatcattt catgtattta
     9241 tgctgtttga aacatcgtta gataacaata gttatattgc acattttaag ctgaagatgg
     9301 aaaaatattc gataatcaaa taaacaatga atcttagaag gtacaatgac gttcactaat
     9361 tcaaatatag ccgctcatgt tggtgagtga tgttcactat agatttatat taatatacaa
     9421 aggttgttaa tttttattaa gcgttaggtt gaatgt
//
LOCUS       DANBWH010000104         9433 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.107, whole genome shotgun sequence.
ACCESSION   DANBWH010000104 DANBWH010000000
VERSION     DANBWH010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9433
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.107"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            88..723
                     /locus_tag="PPY92_001696"
     CDS             88..723
                     /locus_tag="PPY92_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDJ2625690.1"
                     /translation="MSDQVKIPRATLKRLPLYYRFVSSLKSKGIDRVNSKAISDALQI
                     DSATIRRDFSYFGELGKKGYGYNIDSLLDFFKSELSESDMIKIAIVGVGNLGKALLTY
                     NFSIHDDMTITEAFDVKEDVIGQKIGNVIVKDNDELITTLKKEEIDVVILTTPERVAQ
                     KVADELVQAGVKGILNFTPGRINTPSDVQVHQIDLGIELQSLLFFMKNYSE"
     gene            1027..2106
                     /locus_tag="PPY92_001697"
     CDS             1027..2106
                     /locus_tag="PPY92_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HDJ2625691.1"
                     /translation="MWMIFSGLIVGGLLGFVMQRTRFCLTGGFRDMYVQKNNKMFYAL
                     LIAITIQSIGLLILTATDILQIPAHSFPILGTIIGSFIFGIGIVLAGGCATGTWYRAG
                     EGLIGSWIALVLYAVTAAITKTGILKPVMDKINQPTNVNSDMSQTTGIPFWGLVVILT
                     IITIFLVVRTLNNKKVRVAVPKLKQRYTGIRYYLFEKRYHPFIAAIVIGLIALLAWPM
                     SASTGRNDGLGITTPSANLVHFLITGETKFIDWGVFLVLGIFIGSYIAARGSREFKWR
                     LPDKITIRNSAIGGICMGFGASVAGGCSIGNGLVETATMTWQGWIALASMIVGVWTMS
                     HFIFVRPMKKVQQQSAKVKQQTQIV"
     gene            2166..2390
                     /locus_tag="PPY92_001698"
     CDS             2166..2390
                     /locus_tag="PPY92_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDJ2625692.1"
                     /translation="MIHELGTVGMVCPFPLIEAQKKMATLQSGDELKIDFDCTQATEA
                     IPNWAAENGYPITNYEQIDNASWTITIQKV"
     gene            2598..3848
                     /locus_tag="PPY92_001699"
     CDS             2598..3848
                     /locus_tag="PPY92_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HDJ2625693.1"
                     /translation="MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSM
                     AAIVLVTFVWITVGFTISFGNGNLWFGNWEYTFLNHVGFATQEDISPHIPFALFMLFQ
                     MMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFA
                     GGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFT
                     FDNIAMLAFTNTVISASAGAIGWLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVT
                     YLSATIMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGIIGAVLTAVFQSKKAN
                     PDIENGFIYTGDIHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGL
                     DKIVHGEHAYFEGELNRFNKHIRY"
     gene            4031..4981
                     /locus_tag="PPY92_001700"
     CDS             4031..4981
                     /locus_tag="PPY92_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDJ2625694.1"
                     /translation="MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTRIIAEHDY
                     QPNQFAQSLRARQTHLIGAIIPRMNSYAVDETIKGLAKQCQKYESQLILNYTGLNIEA
                     EIQALETLARSKVDGIVLMATDITERHIEVINKMNVPIVIVGQQHEQLHSIVHDDYKA
                     GQIIGEWIGQQGYQQVEVFSVSEKDIAVGIHRKRGLLDQLAKYQIKPNIHETNFTYVE
                     AQKDVANVLENVEQVDAVVGATDTIALAAYKYYSDKNDVMKPHQIYGFGGDPMTQLVS
                     PSIKTIHYNYFEAGQCAMEEIQQMLKKQDMPYSVTVDVNI"
     gene            5130..6614
                     /locus_tag="PPY92_001701"
     CDS             5130..6614
                     /locus_tag="PPY92_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HDJ2625695.1"
                     /translation="MTEWTREERYQRIEDVDTEYFKTLKQQVDQSKFRQQFHIQPETG
                     LLNDPNGLIFYKGKYYVSHQWFPLGAVHGLKYWYNYTSDDLINFKPEGPILNPDTKYD
                     SHGVYSGSAFEYNGHLYYMYTGNHRDNHWQRHASQMIARLKEDGSVEKFPKPVISQQP
                     EGYTSHFRDPKVFKYGEKYYAIIGAQNNDQQGRLLLYNTEDIINWHYLGEINTELDDF
                     GYMWECPDYFNLDNQDVILICPQGIEPKGNQFKNIYQSGYILGKFDIEKLTYEHENFV
                     ELDNGFDFYAPQTFLDEKGRRVLIGWMGLPEIEYPTDNEGWAHCLTIPRVLNVENGQL
                     KQRPYPALEKLRHNKETALGYANKFTRKLHPYEGKQYELIIDILDNDATEVYFELRTS
                     KTSSTLIAYNKRENKITLDRSDSGLLPTNVEGTTRSTILDTPLKQLQIFVDTSSIEIF
                     CNDGERVLTSRIFPTEDALGIKTSTESGQVYLQFTKYDLKDEHK"
     gene            6611..7570
                     /locus_tag="PPY92_001702"
     CDS             6611..7570
                     /locus_tag="PPY92_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDJ2625696.1"
                     /translation="MRRLFSIGEALIDFIPNVTNANLKDVQTFTKQIGGAPCNVACTV
                     QKLGQQAYMITQLGNDAFGDSIIETISSIGVDVSKVYRTNEANTALAFVSLTEAGERD
                     FSFYRKPSADMLFEPSFVNDIDVNENDVVHFCSVDLVDSPMRDAHYQLITKTLNANGT
                     VVFDPNVRLPLWDNAEDLRQTIHTFLPLAHIVKVSDEELEFITGIHDENEAIQSLFTG
                     NVTVVIYTKGADGAAVYLKNGINHYHSGYKVKPVDTTGAGDAFIGAVISRILATDVLN
                     LTQLFENEGEEILAFSNRVAAIVTTKYGAINSLPTLQEVEQAF"
     gene            complement(7758..7867)
                     /locus_tag="PPY92_001703"
                     /pseudo
     CDS             complement(7758..7867)
                     /locus_tag="PPY92_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7939..8655)
                     /locus_tag="PPY92_001704"
     CDS             complement(7939..8655)
                     /locus_tag="PPY92_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDJ2625697.1"
                     /translation="MKIFICEDDPKQRENMVTIIKNYIMIEEKPMEIALATDNPYEVL
                     EQAKNMNDIGCYFLDIQLSTDINGIKLGSEIRKHDPVGNIIFVTSHSELTYLTFVYKV
                     AAMDFIFKDDPAELRTRIIDCLETAHTRLQLLSKDNSVETIELKRGSNSVYVQYDDIM
                     FFESSTKSHRLIAHLDNRQIEFYGNLKELSQLDDRFFRCHNSFVVNRHNIESIDSKER
                     IVYFKNKEHCYASVRNVKKI"
     gene            complement(8674..>9433)
                     /locus_tag="PPY92_001705"
     CDS             complement(8674..>9433)
                     /locus_tag="PPY92_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDJ2625698.1"
                     /translation="LIPYALVFIGLIIFISVVILIMSLFTLKEMKYKRNQEEIETYYE
                     YTLKIEAINNEMRKFRHDYVNILTTLSEYIREDDMIGLRAYFNKNIVPMKDNLQMNAI
                     KLNGIENLKVREIKGLITAKILRAQEMNIPISIEIPDEVSSINLNMIDLSRSIGIILD
                     NAIEASTEIDDPIIRVAFIESENSVTFIVMNKCADDIPRIHELFQESFSTKGEGRGLG
                     LSTLKEIADNADNVLLDTIIENGFFIQKVEIINN"
BASE COUNT         3259 a         1304 c         1755 g         3115 t
ORIGIN      
        1 tttgtttttt aataatctaa caattacgct ctaactcgtt tgaatacata gatataattc
       61 attcaaacac gaaggaggaa atagcgaatg agtgaccaag ttaaaattcc tcgagcaact
      121 ttaaaacgtt tgccgttata ttatagattt gtcagttcat taaaatctaa aggtatagat
      181 cgtgtaaatt caaaagcgat tagcgatgcg ttacaaattg actcggcaac aattcgtcgt
      241 gacttttcat attttggcga attaggtaaa aaagggtacg gatataatat agatagttta
      301 ttggatttct ttaaatctga actaagcgag agtgacatga tcaaaatcgc aattgtcgga
      361 gttgggaacc tagggaaagc tttgctcaca tataactttt caatacatga cgatatgacg
      421 attacagaag cgtttgacgt aaaagaagat gttattggcc agaaaatagg gaacgttatt
      481 gtaaaagata acgatgaatt aataacaaca ttgaagaagg aagaaataga tgttgtgatt
      541 ctaactacac cagaaagagt tgcacagaaa gttgcagatg aactcgtcca agctggtgtg
      601 aaaggtattt taaacttcac tcctggtaga attaatacgc cttcagatgt gcaagtacat
      661 caaattgact taggtataga attacagtca ttattattct ttatgaaaaa ttacagtgaa
      721 taaatgtgat gttagctttg aatgattaac cttgttatat ctttaattaa ttatagaaca
      781 gtctgggact aagtatgtct caggcttttt ctgttatgtt tgaaaatgaa aggaaaaact
      841 gttgtgttga taaatatagt ataggttaat aaattaacac gtagagatat aatgatgaat
      901 cgtaattaat cgccttcctt atatgaatat aatataaata tcaaaaatat tgtgtataag
      961 gttgaaaaca agaaaatcat cacctataat acatactaaa cttatcggaa atgaggggtt
     1021 aggattatgt ggatgatttt tagtggctta attgtcgggg gactcttagg ttttgtcatg
     1081 caaagaacaa gattttgttt aacaggtggc tttcgagata tgtatgtgca aaagaataat
     1141 aagatgttct atgcattatt aatcgctatt actattcaaa gtataggatt attgattttg
     1201 acggcaacag atattttaca aattcctgca catagttttc caatattggg aacaattata
     1261 ggttctttta tttttggaat tggaatagta ttggctggag ggtgtgcaac aggtacttgg
     1321 tatcgcgctg gtgaagggct aattggtagt tggattgcat tagtattata tgctgttact
     1381 gcagcaatca ctaaaacagg gattttaaag ccagtaatgg ataaaattaa tcaaccaacg
     1441 aatgtaaata gtgatatgtc tcaaacaact ggcattccgt tttggggatt agtcgttata
     1501 ttaactataa tcaccatttt tctagttgta agaacactca ataataagaa agttcgagtt
     1561 gctgttccaa aactaaagca acgttataca ggtattagat attacctttt cgaaaaacga
     1621 taccatccat ttattgcagc aattgtaatt ggacttatcg cactcttagc ttggccaatg
     1681 agtgcatcaa ctggaagaaa tgacggttta ggtataacaa cgccttcagc aaatttagta
     1741 cactttttga ttacaggtga aactaaattt attgattggg gtgtcttttt agttctagga
     1801 attttcattg gttcatatat tgcagctaga ggatcaagag aatttaaatg gcgattgcca
     1861 gacaagatta caatacgaaa cagtgccatt ggtggcatat gtatgggatt tggtgcgtca
     1921 gttgctggtg gttgttctat cggtaacggt ttggttgaaa cggcaacgat gacttggcaa
     1981 ggatggattg cgctagcatc gatgatagtt ggtgtatgga caatgagtca ttttatcttt
     2041 gttcgtccaa tgaaaaaagt acaacaacaa tccgcaaagg ttaaacagca aacgcaaata
     2101 gtatagaaga ttattatgca aatgatgttg atcaaataaa agtgattgga aaaggagaaa
     2161 taattatgat acacgaatta ggtacagtag gaatggtatg tccatttccg ttaattgaag
     2221 cgcaaaagaa aatggcaaca ttgcaatctg gagatgaatt aaaaattgat tttgattgca
     2281 cgcaagcgac ggaagccatt ccaaattggg ctgcagaaaa tggttatcct ataacaaact
     2341 atgaacaaat tgataatgct tcatggacaa ttacaattca aaaagtttaa cgttatcatt
     2401 ttaacaataa aatagatatt agattctatg gctacttccg ctaatttaaa agtgagtaag
     2461 tagtcttttt tgttttagtt catgaaatca tttttatata gtgtggcata ttttatttca
     2521 aaagatgtaa taaaacttaa cgcatttttg ctttttataa attgtcagat tattatgaaa
     2581 acaagggagt ggtaagtatg aatcttaacg atacgatatt tatgtttttg tgtacattat
     2641 tagtttggtt aatgacacca ggattaagtt tattttatgg tgggttagtt caatctaaaa
     2701 atgcgcttaa tactgtcatg caaagtatgg cagcaattgt gcttgttaca tttgtatgga
     2761 taacagttgg ttttacaatt agttttggga atgggaattt atggttcgga aattgggaat
     2821 atacttttct taatcatgta ggttttgcga ctcaagaaga tattagccca catattcctt
     2881 tcgctttgtt tatgttattt caaatgatgt tttgtacgat tgcaatttct attttatctg
     2941 gttcaatcgc tgagaaaatg aagtttattc cttatttatt attcgtagta atatggactg
     3001 ctcttgtata cagtccagta gcacattggg tttggggcgg cggttggatt aataaactcg
     3061 gtgtattaga tttcgctgga ggtacggttg ttcatattac atcaggtgtt tctggtttag
     3121 tattagctat tatgattgga aaaggaaaca aacattctga atcaacacca cataatctta
     3181 tcattacgtt gattggcggt atattcgtgt ggattggttg gtatggattt aatgtaggta
     3241 gtgcttttac atttgataat attgcgatgc ttgcatttac aaatactgtc atttcagcca
     3301 gtgcaggtgc tataggttgg ttaattttag aatatatttt taaaaagacg acaagtttac
     3361 ttggactttt actcggtgca ttagcaggat tagttgttat tactcctgca gcaggatatg
     3421 taacatatct tagtgcaaca ataatggctt taataggagg tatctgttgt tatattgtca
     3481 ttaattacat caaggtaaaa ctaaaatatc atgatgcatt agatgcattt ggtattcatg
     3541 gtgttggtgg tattattggt gctgttttaa cagcagtttt ccaaagtaaa aaagccaatc
     3601 ctgacattga gaatggcttt atttatactg gtgacataca tattatactt gtacaaatat
     3661 tatgtgtaac agcagttgta atttttagta tcgtcatgac gtttattatt gcgaaagtaa
     3721 ttaaattaat tacaccatta tctgttacgg aacaagaaac gaatatagga ttagacaaga
     3781 ttgttcacgg tgaacatgct tactttgaag gtgagctaaa tagattcaat aaacatattc
     3841 gatattagaa tatatttaca tagaatattc attgtcctga catttaacta aaggttgatg
     3901 ttgggacatt ttgttataca aaagttttat tttgaaatct ttttatgaaa gaagcagaaa
     3961 tattatttaa agcggttaca catatgctaa aataaggcta agtgtcacaa ataatgatag
     4021 gtgaataagt atgaaaaata tatctgatat tgccaaattg gcaggcgttt caaaaagcac
     4081 agtatctaga tttttaaata atggatctgt cagtaaaaaa acaagtgaaa aattaacaag
     4141 aattatagca gaacatgact atcaaccgaa tcaatttgct caaagtttaa gagcgagaca
     4201 aacacacctt attggtgcca ttatacctag aatgaattca tatgcagtag atgaaacaat
     4261 caaaggattg gcaaaacaat gccaaaaata tgaatcacaa ttaattttaa attacacagg
     4321 tttaaatatc gaagcagaaa tacaagcgct tgaaacatta gcacgcagta aagtagatgg
     4381 tattgtttta atggctacag acataacaga gagacatatt gaagtcatta ataaaatgaa
     4441 tgtaccaatc gttattgttg gtcaacaaca tgaacaactt catagtattg tgcatgatga
     4501 ttataaagca ggtcaaatta taggcgaatg gattggtcaa cagggatatc aacaagttga
     4561 agtgtttagt gtaagtgaaa aagatattgc agttggtata catagaaaac gtggtttact
     4621 tgaccagtta gctaaatacc aaattaaacc taatatacat gaaacaaatt ttacttatgt
     4681 ggaagcacaa aaagatgttg caaatgtttt ggaaaatgtg gagcaagtag atgcggttgt
     4741 tggagcaact gatacgattg cattagctgc ctataaatat tattctgata aaaatgatgt
     4801 tatgaaacca catcaaatat atggttttgg tggtgaccca atgacacaat tagtgtctcc
     4861 atcgataaaa acaattcatt ataattattt tgaagctggc caatgcgcga tggaagagat
     4921 acaacagatg cttaaaaagc aagatatgcc atatagcgtc acagtagatg ttaatattta
     4981 gacgctgtat tttttaaaac aaatgtggaa ccgataccat ataactataa atggataggt
     5041 taaaagttaa agaacgtagg taaaatttgc tataatagaa tataaattgt taacagcata
     5101 aattataaaa ggaggactgg gtaaatatta tgaccgaatg gactagagaa gaacgttatc
     5161 aacgaatcga ggacgttgat actgagtatt ttaaaacatt aaaacaacaa gttgatcaat
     5221 caaaatttcg tcaacaattt catatacaac cagaaacagg tttattaaat gatcccaacg
     5281 gacttatttt ttataaaggg aagtattatg tttcacatca atggttccca ttaggcgcag
     5341 tacatggctt aaagtattgg tataactaca cgagtgatga cttaataaac tttaaacctg
     5401 aagggccaat attaaatcca gatactaaat atgacagcca tggtgtttat agcggtagcg
     5461 cttttgaata taacgggcat ttatattata tgtacacagg aaatcatcga gataatcatt
     5521 ggcaacgaca tgcgagtcag atgatcgcac gattgaaaga agacggttca gttgaaaagt
     5581 ttccaaagcc agtaattagc cagcaaccag aaggatatac aagtcatttt agagatccta
     5641 aagtttttaa atatggtgag aaatattatg caatcattgg cgcacaaaat aatgatcagc
     5701 aaggtcgatt attactttat aatactgaag atataattaa ttggcattat ttaggtgaaa
     5761 taaatacaga gttagatgat tttggatata tgtgggaatg cccagattac tttaatttag
     5821 ataatcaaga tgtcatactt atttgtccac aaggtattga accaaaaggc aatcagttca
     5881 aaaatattta tcaaagtggt tatatacttg gaaagtttga tattgaaaag ttaacatatg
     5941 aacatgaaaa ttttgtcgag cttgataatg gctttgattt ctatgcacct caaacatttt
     6001 tagatgaaaa aggccgacga gtactaattg gatggatggg gttaccggaa atcgaatatc
     6061 ctactgataa tgaaggatgg gcccattgct taacaattcc tcgtgtatta aatgtagaaa
     6121 atggtcaact taagcaacgt ccatatccag cgttggaaaa attacgtcac aataaagaga
     6181 cagcattagg ctacgcaaat aaatttactc gaaaattaca tccgtatgaa ggtaaacagt
     6241 atgaattaat catagatatt ttggataatg atgctaccga agtgtacttt gaattacgta
     6301 catctaagac ttcttcaaca ttaattgctt ataacaagcg tgaaaataaa ataacattag
     6361 atcgcagcga cagtggttta ttgccgacaa atgttgaagg tacgacgcgt agtacgatat
     6421 tagacacgcc attaaagcaa ttacaaattt ttgttgatac atctagtata gaaattttct
     6481 gtaatgatgg ggaacgtgtt ttgacatctc gaattttccc gacagaagac gcactaggta
     6541 ttaaaacatc aacagaatca gggcaagtat atttacaatt tactaaatat gatttaaagg
     6601 atgaacataa atgagacgtc tattttcaat aggagaagcg ttaattgatt ttattccaaa
     6661 tgttacaaat gcgaatttaa aagatgttca aacatttacg aagcaaattg gtggcgcgcc
     6721 atgcaatgta gcttgtacag ttcaaaagtt aggacaacaa gcgtatatga ttacacaatt
     6781 aggtaatgat gcatttggag atagcattat tgaaacgata tcaagtattg gtgtagatgt
     6841 atcaaaagtg tatcgcacga atgaagcaaa cactgcgtta gcttttgtga gtttgactga
     6901 agcgggcgag cgagattttt ctttttatcg taagccctct gctgatatgt tatttgaacc
     6961 aagttttgtg aatgatattg atgtgaatga aaatgatgta gtacattttt gttctgttga
     7021 tttggtcgat agtccgatga gagatgcaca ttatcaattg attaccaaaa cattaaatgc
     7081 aaatggtact gtcgtatttg atcctaatgt aagattgcca ttatgggata acgctgaaga
     7141 tttaagacaa actattcata cctttttgcc attggcgcat attgtaaaag tttcagatga
     7201 agaattagaa tttattacag gtattcatga tgaaaatgaa gccattcaat cattatttac
     7261 aggtaatgtt actgtagtca tttatacgaa gggagcagat ggtgctgccg tatatttgaa
     7321 aaatggtatt aatcattatc atagtggcta taaggtaaaa cctgttgata caactggggc
     7381 aggggatgct tttattggtg cagttattag caggatttta gcaaccgatg tgttaaattt
     7441 aactcagtta tttgaaaatg aaggtgagga aattttagcg tttagcaatc gcgttgctgc
     7501 aatagtgaca acgaagtatg gtgccattaa tagtttgcca acattacaag aggttgaaca
     7561 agcattttaa ataattcatt tataaaacag tgtaggaaac aagttgaatc ttgtgctaca
     7621 ctgtttttta aaacaaaata agattcacgg ggtaggaaat tgtagcattt tatttatgga
     7681 aactgtcaat ttcaaattta gtatattttg ttaaaaattg cgccatagga ttgtagagtg
     7741 aaatagaaat tgataaatta tgtagtaata ggaataggta tacaaagaaa cgttacatca
     7801 ttttttaatt cacgcgtcat atttaatttt gttattaaac aattaaaaag ccagctatac
     7861 agtgcatttg ctagttatct tgttaaaatc caacaagatt tacaattgaa tacgccgtta
     7921 actgacttta ttatcttatt atattttttt aacgtttctc accgatgcat agcagtgttc
     7981 tttattttta aaatagacaa ttcgctcttt cgaatctata gattcaatat tatggcgatt
     8041 gacgacaaag ctattatgac atctaaagaa acgatcatct aattgactca gttcttttaa
     8101 attaccataa aattcaattt gacggttatc taaatgggca atgagtctgt gagattttgt
     8161 tgatgattca aaaaacataa tatcatcata ttgaacatac actgaattac tgccacgttt
     8221 taattcaatc gtttcaacgc tattatcttt agacaacaat tgtaagcgtg tatgtgcagt
     8281 ttctaaacaa tctataattc gagttcttaa ttctgctgga tcatctttaa aaataaaatc
     8341 catcgctgca actttgtaga caaatgttaa ataggtaagt tcactgtgac tcgtaacgaa
     8401 aataatgtta ccaactgggt catgcttacg aatttcactg cctaatttga taccattaat
     8461 atcagttgaa agttgaatat ctaaaaagta acagcctatg tcattcatat ttttagcttg
     8521 ctcaagcacc tcataaggat tatcagttgc gagggcaatt tccataggct tttcttctat
     8581 cattatataa tttttaataa tggtaaccat gttttctctt tgttttggat cgtcttcgca
     8641 aatgaaaatt ttcatacatt cacatcctta tggctagttg ttaataattt caactttttg
     8701 aataaagaaa ccattttcga taattgtatc taataagaca ttgtctgcat tatcagcaat
     8761 ttcttttaaa gttgatagac ctaaaccacg accttcacct ttagtagaaa aactttcttg
     8821 gaacaattca tgaatgcgtg gtatatcatc agcgcattta ttcataacaa taaacgttac
     8881 tgaattttca ctttcaataa atgcaacgcg aatgataggg tcatcaattt cagttgatgc
     8941 ctcaattgca ttatcaagaa taataccaat actgcgactt aaatcgatca tattcaagtt
     9001 aatgctactt acttcatcgg gtatttcgat actaattgga atattcattt cttgtgcacg
     9061 taaaattttc gcagtaatta agccttttat ctcacgtact ttaagattct cgataccatt
     9121 taatttaata gcattcatct gcaaattatc tttcataggt acaatatttt tattgaaata
     9181 agcgcgtaga ccaatcatgt catcttcgcg aatgtattct gaaagtgtcg ttaagatatt
     9241 gacataatca tgacggaact tgcgcatttc attattaatt gcttcaatct ttaatgtgta
     9301 ttcgtaatag gtctcaattt cttcttgatt tcgtttgtat ttcatctctt taagggtgaa
     9361 aagcgacatt ataagtatta caacagagat aaaaattatt aatcctataa atacaagtgc
     9421 atatggaatt aag
//
LOCUS       DANBWH010000105         9301 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.108, whole genome shotgun sequence.
ACCESSION   DANBWH010000105 DANBWH010000000
VERSION     DANBWH010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9301
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.108"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..396
                     /locus_tag="PPY92_001706"
     CDS             <1..396
                     /locus_tag="PPY92_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HDJ2625699.1"
                     /translation="QNSGFRNEYYYLTAIPYTLDEYKRYFQPLIKEDDKSFRDGMRNS
                     KKQLKDKSRPYVVTTLFSTKDNFTKDNTIDEMIDFSEVLKKKKNIPHDLNVSLQISNK
                     YINTKRPNYSKKEVIEVGVFNHEKANTND"
     regulatory      602..736
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            798..1343
                     /locus_tag="PPY92_001707"
     CDS             798..1343
                     /locus_tag="PPY92_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDJ2625700.1"
                     /translation="MQQNKRLITISMLSAIAFVLTFIKFPIPFLPPYLTLDFSDVPSL
                     LATFTFGPVAGIVVALVKNLLNYLFSMGDPVGPFANFLAGASFLLTAYAIYKNKRSTK
                     SLITGLIIATIVMTIVLSILNYFVLLPLYGMIFNLADIANNLKVIIVSGIIPFNIIKG
                     IVISIVFILLYRRLANFLKRI"
     gene            complement(1449..1697)
                     /locus_tag="PPY92_001708"
     CDS             complement(1449..1697)
                     /locus_tag="PPY92_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDJ2625701.1"
                     /translation="MAKYTIVDMDTCIACGACGAAAPDIYDYDDEGIAFVILDDNQGT
                     AEVPEELYEDMEDAIDGCPTDSIKIADESFDGDALKFE"
     gene            1805..2758
                     /locus_tag="PPY92_001709"
     CDS             1805..2758
                     /locus_tag="PPY92_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDJ2625702.1"
                     /translation="MQHIIKTALQQTFNYKTNKSIYNILVGKKSHQTFFDACSQQQLS
                     LYHSLPLLKYPSFELFLEKINEFNAEMEIMLHPRYTFESMGQTFQAIQLLVQTMSNTK
                     QHVFHFVPISQNNKIQETVKIVYNYIKENQLQIDFENELHNLFKAITLKGPCYLHYYL
                     QGYDEPMYTRQQVSLIEKLSQQQLFEYEMNNLVTMMFELESGEYTILSKIIMKPTLLN
                     QTYITYTKLLEQFTMEDIAAQQQGKINTIEDHVLEILIKGYMSNYDDYVELEDQLQFL
                     NFYQQHRGERLKFYKEQFDTLSYFQLKVLIVGFERGDLNVA"
     gene            2748..4127
                     /locus_tag="PPY92_001710"
     CDS             2748..4127
                     /locus_tag="PPY92_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDJ2625703.1"
                     /translation="MLHDILRNKFGFENFKPGQQEIIESIMSQQHTLGILPTGSGKSL
                     CYQIPTYLSGKPTLIISPLISLMDDQVMQLKINGEKRVTCIHSGMDEIEKKHNIKCLL
                     HSRFIFLSPEFLLQPSNFKLISMIDFGMIVLDEAHCLSEWGYDFRPHYALIGKVTKHF
                     KEAVVLALTATAPPHLQDDLTEMLAIQFNVIKTTMNRPNISFKHLNFHDDEDKIEWLL
                     PFLQQSGPTIIYVSSKKMCLNLAQLIYDSGFLTGIYHGDMNYQERHTVQQQFLNNDIP
                     IIVATSAFGMGINKKDIRTIIHFHLSTSPSNYIQEIGRAGRDGELSQAISLFQPDDKY
                     ILETLLFADMITEEDVQNFEIGEFLAPDKQDVLTTLHSFYSIGALKQIFKQSFKRKQL
                     GFFRMIGYCKLDQCRRKYLLEFFGEYPPAQDRCCDNDSNITDIVILNKKKVIRSIGFD
                     EKLQNLFLR"
     gene            4280..5740
                     /gene="ebpS"
                     /locus_tag="PPY92_001711"
     CDS             4280..5740
                     /gene="ebpS"
                     /locus_tag="PPY92_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elastin-binding protein EbpS"
                     /protein_id="HDJ2625704.1"
                     /translation="MSNNFKDDFEKNRQSIDTNSHQDHTEDVEKDQSELEHQDTIENT
                     EQQFPPRNAQRRKRRRDLATNHNKQVHNESQTSEDNVQNEAGTIDDRQVESSHSTESQ
                     EPSHQDSTPQHEEEYYNKNAFAMDKSHPEPIEDNDKHETIKDAENNTEHSTVSDKSIA
                     EQSQQPKPYFATGANQANTSKDKHDDVTVKQDKDESKDHHSGKKGAAIGAGTAGVAGA
                     AGAMGVSKAKKHSNDAQNKSNSDKSNNSTEDKASQDKSKDHHNGKKGAAIGAGTAGLA
                     GGAASKSASAASKPHASNNASQNHDEHDNHDRDKERKKGGMAKVLLPLIAAVLIIGAL
                     AIFGGMALNNHNNGTKENKIANTNKNNADESKDKDTSKDASKDKSKSTDSDKSKEDQD
                     KATKDESDNDQNNANQANNQAQNNQNQQQANQNQQQQQQRQGGGQRHTVNGQENLYRI
                     AIQYYGSGSPENVEKIRRANGLSGNNIRNGQQIVIP"
     gene            5865..5981
                     /locus_tag="PPY92_001712"
     CDS             5865..5981
                     /locus_tag="PPY92_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625705.1"
                     /translation="MLFNKVLSRILAYQLNFLNSHQKDKTPQSTNLTRENKK"
     gene            6142..7128
                     /locus_tag="PPY92_001713"
     CDS             6142..7128
                     /locus_tag="PPY92_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HDJ2625706.1"
                     /translation="MQKVESIIIGGGPCGLSAAIEQKRKGIDTLIIEKGNVVESIYNY
                     PTHQTFFSSSDKLSIGDVPFIVEESKPRRNQALVYYREVVKHHQLKVNAFEEVLTVKK
                     MNNKFTITTTKDVYECRFLTIATGYYGQHNTLEVEGADLPKVFHYFKEAHPYFDQDVV
                     IIGGKNSAIDAALELEKAGANVTVLYRGGDYSPSIKPWILPNFTALVNHEKIDMEFNA
                     NVTQITEDTVTYEVNGESKTIHNDYVFAMIGYHPDYEFLKSVGIQINTNEFGTAPMYN
                     KETYETNIENCYIAGVIAAGNDANTIFIENGKFHGGIIAQNMLAKKQTPLES"
     gene            complement(7243..8211)
                     /locus_tag="PPY92_001714"
     CDS             complement(7243..8211)
                     /locus_tag="PPY92_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDJ2625707.1"
                     /translation="MKHLLVIHTGGTISMSQDQSNKVVTNDINPISMHQDVINHYAQI
                     DELNPFNVPSPHMTIQHVKQLKDIILEAVTNKYYDGFVITHGTDTLEETAFLLDLILG
                     IEQPVVITGAMRSSNEIGSDGLYNYISAIRVASDEKARHKGVMVVFNDEIHTARNVTK
                     THTSNTNTFQSPNHGPLGVLTKDRVQFHHMPYRQQALENVNDKLNVPLVKAYMGMPGD
                     IFSFYSREGIDGMVIEALGQGNIPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAY
                     DGGGYQLAQQGFIFSNGSNGPKARLKLLVALSNNLDKAEIKSYFEL"
     gene            8288..8947
                     /locus_tag="PPY92_001715"
     CDS             8288..8947
                     /locus_tag="PPY92_001715"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDJ2625708.1"
                     /translation="MKAINIALDGPAAAGKSTIAKRVASELSMIYVDTGAMYRALTYK
                     YLKLNKTEDFAKLVDQTTLDLTYKADKGQCVILDNEDVTDFLRNNDVTQHVSYVASKE
                     PVRSFAVKKQKELAAEKGIVMDGRDIGTVVLPDADLKVYMIASVEERAERRYKDNQLR
                     GIESNFEDLKRDIEARDQYDMNREISPLRKADDAVTLDTTGKSIEEVTDEILAMVSQI
                     K"
     gene            8978..9130
                     /locus_tag="PPY92_001716"
     CDS             8978..9130
                     /locus_tag="PPY92_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625709.1"
                     /translation="MQRLTHIQNECADNDLLKLKGDFYSMMNESCLFIFGKSSCVSEL
                     CKFFKK"
BASE COUNT         3477 a         1445 c         1580 g         2799 t
ORIGIN      
        1 cagaatagtg gttttagaaa tgagtattat tatttgactg caattccata tactttagat
       61 gaatataaaa ggtattttca acccttaatc aaagaggatg ataagtcttt tagggacggt
      121 atgagaaact caaaaaaaca acttaaagat aaatcacgtc catatgttgt tacaacttta
      181 tttagtacta aagataattt tactaaagat aatacaatag acgagatgat tgatttttct
      241 gaagtactaa aaaagaaaaa gaatattcct catgatttga atgtttctct tcaaatttct
      301 aataaatata taaatacaaa aagacctaac tatagtaaga aggaagtaat agaagtaggt
      361 gtatttaatc atgaaaaagc taacacgaat gattaaacta ataacaataa aaaggtaggt
      421 tcaaaaaagc catctcaaca acagttaaaa ccacaaacca atcaaaccta tccaaccaat
      481 gaactcaaat caccaatgta tcactctcca ataatgtaac tatgatctaa ttaaagcata
      541 gttattgagg ttttgtgata tatagtataa aattaatgag aattaaattt aataatgtaa
      601 aattcatctt cggggtcggg tgtaattccc aaccggcagt aaataaagcc tgcgacctgc
      661 tagtatgtat catattagtg gctgatctag tgagattcta gagccgacag tatagtctgg
      721 atgggagaag atggaggttt tttgttgtgc aataatcctc ctattcttac gagatgaatg
      781 gaaggagaaa attgaatatg caacaaaata aacgtcttat cacaataagt atgttgagcg
      841 cgattgcgtt tgtgttaact tttatcaagt ttcctatacc atttttgcca ccatacttaa
      901 ctttagattt tagtgatgta ccgtcactac tagctacatt tacgtttgga ccagttgccg
      961 gtatcgtagt tgcactggtt aaaaacttgc tgaactactt atttagtatg ggcgatccag
     1021 ttgggccgtt tgctaacttt ttagcaggcg caagtttctt attaactgct tacgccatct
     1081 ataaaaataa acgttcaaca aaatctttga ttactggatt aatcattgca acaatcgtta
     1141 tgactatcgt gttgagtatt ttgaactatt tcgttctatt acctttgtac ggtatgatat
     1201 ttaacttagc tgatatcgca aataatctta aagtaatcat tgtatcagga attataccat
     1261 tcaatattat taaaggtatc gtcatttcta ttgtatttat tttactatat agaaggcttg
     1321 cgaatttctt gaaaagaatt taatcaaatt aaagcaaaat aatatacaca taataataaa
     1381 aagcaggtga ctatcaataa acgatagctt gcctgctttt tctatagaac atttgtctaa
     1441 aaaatcaatt attcaaattt taaagcgtcc ccatcaaatg attcgtctgc aattttaata
     1501 gaatctgtag ggcatccatc aattgcatct tccatatctt catataattc ctcaggtact
     1561 tctgcagtac cttggttatc gtcaaggatt acgaaagcaa taccttcgtc gtcgtaatca
     1621 tatatatctg gcgctgctgc accgcatgca ccacatgcaa tacaagtatc catatcaacg
     1681 attgtatatt ttgccaatgt cttcgcctcc tttgataaaa atgctaaaat agtaatgtga
     1741 ctaaaatttt agacagcatc atttttattt tcaaattatc cgttttacag agtgagggtt
     1801 aaatttgcaa cacattataa aaacagcatt acaacaaaca tttaactata aaacaaataa
     1861 aagtatttac aatatcttag ttggtaagaa atctcaccaa accttttttg acgcttgtag
     1921 tcaacaacag ttgtcattat atcacagttt accactatta aaatatccgt cttttgagct
     1981 atttttagaa aaaatcaatg aatttaatgc tgaaatggaa atcatgttgc atcctagata
     2041 tacatttgaa agcatggggc aaacatttca agcaattcaa ctattagtgc aaaccatgtc
     2101 taataccaaa caacatgttt ttcattttgt accaatctct caaaataata agatacaaga
     2161 aactgtaaag attgtctata actacattaa agaaaaccaa ttacaaattg actttgaaaa
     2221 tgaactacat aacttgttta aagcaataac tttaaaaggg ccatgttact tacattatta
     2281 tttgcaaggc tatgatgaac caatgtatac gagacagcaa gttagtttaa tagaaaaact
     2341 atctcaacag caattgtttg aatacgaaat gaataattta gtgacaatga tgtttgaatt
     2401 agaaagtgga gaatatacta ttttatcaaa aataataatg aaacctacat tattaaatca
     2461 aacttatatt acttatacaa aattgcttga acaattcacg atggaagata tagcggctca
     2521 acaacaaggt aaaatcaata ctatcgaaga tcatgtactt gaaatcttaa tcaaaggtta
     2581 catgtctaat tacgatgatt atgttgaact agaagatcaa ctccagtttt tgaattttta
     2641 tcaacaacat cgtggcgaac gattaaaatt ttacaaagaa caatttgaca cgttatcata
     2701 ttttcaatta aaagtattaa tcgttggatt tgaaagaggt gatctgaatg ttgcatgata
     2761 ttttacgaaa caaatttggc ttcgagaatt ttaaaccggg acaacaggaa attatagaaa
     2821 gtataatgtc tcaacaacac actctaggta ttcttccaac tggaagtgga aagagtttgt
     2881 gttatcaaat acctacgtat ttatcaggta agccgacatt aattatctca ccgttaatat
     2941 ctttaatgga tgaccaagtt atgcagttga aaataaatgg agaaaaacgt gtaacatgta
     3001 ttcactctgg tatggatgaa attgagaaaa agcataatat taaatgttta ctacatagcc
     3061 gcttcatctt tctaagtcca gaatttctcc tgcaaccgtc aaattttaaa ttaatatcta
     3121 tgatagactt tggcatgatt gttctagatg aagcacattg cctatctgaa tggggatatg
     3181 atttcagacc acattatgct ctaataggaa aagtaacaaa gcattttaaa gaagcggttg
     3241 tcttagcgtt gacagcaact gcaccaccgc atttacaaga tgatttgacg gaaatgttag
     3301 cgattcaatt caacgttatt aaaactacaa tgaatcgccc aaacataagc tttaagcatc
     3361 ttaattttca tgatgatgaa gataaaattg agtggttgct gccgtttcta caacagtcgg
     3421 gaccaacgat tatctatgtc tcatcgaaaa agatgtgtct gaatttagcg caacttattt
     3481 atgattcagg ttttcttact ggcatttatc atggtgatat gaattatcaa gagcgacaca
     3541 cagttcaaca acaattttta aataatgata ttccgattat agtcgcaacg agtgcttttg
     3601 gtatgggtat taataaaaaa gatattcgca caatcattca ctttcatctt tcaacaagtc
     3661 cttctaacta cattcaagaa attggccgtg caggtcgcga tggtgaacta agtcaggcaa
     3721 ttagtttatt tcaaccggac gataaatata ttttagaaac gttattattt gcagatatga
     3781 taacagaaga agatgtacaa aatttcgaaa taggagaatt tttagcaccc gataaacaag
     3841 acgttttgac aacgttgcat tcattctata gtatcggcgc cttgaaacag atatttaagc
     3901 aatcatttaa acgaaagcaa ttaggattct ttcgcatgat tggctattgc aaattggatc
     3961 aatgtagacg gaagtattta ttagaatttt tcggtgaata tccaccggca caagatcgat
     4021 gttgtgacaa tgattctaat ataactgata tcgtaatttt aaataagaag aaggtaatta
     4081 gaagtattgg atttgatgaa aagttgcaaa atttatttct cagatagtat tactttacta
     4141 aaagaaaatt gacaagctat aattagtgta tacacaattg aaaaatgatt gaaataattt
     4201 tgaaaaatat acataaacat atgtcatgtg ggtatatttt atgtaaaatc attgtaatag
     4261 aatagaaagg aagatggcta tgtctaataa ttttaaagat gactttgaaa aaaatcgtca
     4321 atcgatagac acaaattcac atcaagacca tacggaagat gttgaaaaag accaatcaga
     4381 attagaacat caggatacaa tagagaatac ggagcaacag tttccgccaa gaaatgccca
     4441 aagaagaaaa agacgccgtg atttagcaac gaatcataat aaacaagttc acaatgaatc
     4501 acaaacatct gaagacaatg ttcaaaatga ggctggcaca atagatgatc gtcaagtcga
     4561 atcatcacac agtactgaaa gtcaagaacc tagccatcaa gacagtacac ctcaacatga
     4621 agaggaatat tataataaga atgcttttgc aatggataaa tcacatccag aaccaatcga
     4681 agacaatgat aaacacgaga ctattaaaga tgcagaaaat aacactgagc attcaacagt
     4741 ttctgataag agtatagctg aacaatctca gcaacctaaa ccatattttg caacaggtgc
     4801 taaccaagca aatacatcaa aagataaaca tgatgatgta actgttaagc aagacaaaga
     4861 tgaatctaaa gatcatcata gtggtaaaaa aggcgcagca attggtgctg gaacagcggg
     4921 tgttgcaggt gcagctggtg caatgggtgt ttctaaagct aagaaacatt caaatgacgc
     4981 tcaaaacaaa agtaattctg acaagtcgaa taactcgact gaggataaag cgtctcaaga
     5041 taagtctaaa gatcatcata atggcaaaaa aggtgcagcg atcggtgctg gaacagcagg
     5101 tttggctgga ggcgcagcaa gtaaaagtgc ttctgccgct tcaaaaccac atgcctctaa
     5161 taatgcaagc caaaaccatg atgaacatga caatcatgac agagataaag aacgtaaaaa
     5221 aggtggcatg gccaaagtat tgttaccatt aattgcagct gtactaatta tcggtgcatt
     5281 agcgatattt ggaggcatgg cattaaacaa tcataataat ggtacaaaag aaaataaaat
     5341 cgcgaataca aataaaaata atgctgatga aagtaaagac aaagacacat ctaaagacgc
     5401 ttctaaagat aaatcaaaat ctacagacag tgataaatca aaagaggatc aagacaaagc
     5461 gactaaagat gaatctgata atgatcaaaa caacgctaat caagcgaaca atcaagcaca
     5521 aaataatcaa aatcaacaac aagctaatca aaatcaacaa cagcaacaac aacgtcaagg
     5581 tggtggccaa agacatacag tgaatggtca agaaaactta taccgtatcg caattcaata
     5641 ctacggttca ggttcaccgg aaaatgttga aaaaattaga cgtgccaatg gtttaagtgg
     5701 taacaatatt agaaacggtc aacaaatcgt tattccataa tataactata taaattgtaa
     5761 ctgaactgct atatacaaac gtgcatatta taagtatctc taaatatttt ggagaactta
     5821 aattgcgctt ataagtatgt agcggttttt tcatttttca aagtttgtta tttaacaagg
     5881 tcttgtctcg aatattggca tatcaattaa actttttaaa tagtcatcaa aaagataaaa
     5941 caccacaatc aacaaattta acgagggaga ataaaaaata aatcaacata ttaaattgta
     6001 gtgttattca actccgtagc taacaattct ctatgcacat taaacaaatt gtcaaaaata
     6061 tatcataaat cttcaagcac agacttagcg catcaatcac tgaactgtta taatagtttg
     6121 ggattaaagg aggccgaaac aatgcaaaaa gttgaaagta tcataattgg tggagggcca
     6181 tgcggattaa gtgcggctat tgaacaaaaa agaaaaggta ttgatacctt aattattgaa
     6241 aaggggaatg tcgttgaatc aatctataat tatcctactc accaaacatt tttctcatca
     6301 agtgataaat taagtattgg ggacgtaccg tttatcgttg aagaaagtaa accaagacgt
     6361 aatcaagcgc tagtttatta ccgagaagtt gtaaaacatc atcaattaaa agtaaatgca
     6421 tttgaagaag tattaactgt taaaaaaatg aataataaat ttactattac tacgacgaaa
     6481 gatgtttatg aatgtcgatt tttaacaatc gcgacaggct attatggtca gcataataca
     6541 ttagaagttg aaggagcgga tttacctaaa gtgttccatt attttaaaga ggcacatccg
     6601 tattttgatc aagatgttgt tattattggt ggtaagaatt cggctatcga tgctgctttg
     6661 gagttggaaa aagctggtgc taacgtgacg gttctatatc gtggtggaga ttattcgcct
     6721 tcaattaaac cgtggatact tccaaatttc acagcattag taaatcatga aaaaattgac
     6781 atggaattta atgctaatgt tacccaaata actgaagata ctgtgactta tgaagtaaat
     6841 ggtgaaagta aaacaataca caatgattat gtatttgcga tgattggtta tcatcccgat
     6901 tatgaatttt taaaatctgt aggcattcaa attaatacaa atgaatttgg aacagcgcct
     6961 atgtataata aagaaacata cgaaacaaat atcgaaaatt gctatattgc aggtgtaatt
     7021 gctgcaggga acgatgcgaa taccattttt attgaaaatg gtaaattcca cggtggcatt
     7081 attgctcaaa acatgctagc taagaaacaa acgccgttag aatcataaaa ataaaggtct
     7141 atgtactaaa taacttagtt ttacaacgac tgacattcat gatatgtcaa atgaggttga
     7201 tgactaatta ttgtacatag acctttttat gttacgtatt cattataatt caaaatatga
     7261 tttgatttca gctttatcta aattgttgct taacgcaact aataatttta atcttgcttt
     7321 tggaccattc gaaccgttag aaaaaataaa accttgttgt gcgagttggt aaccaccacc
     7381 atcgtatgcg taagttggac tcacaatacc attaaaggaa cgtgaaacta gcacaatagg
     7441 tatatttaaa gatactaatt gttgaatgcc ttctaatgcg cttggaggta tgttgccttg
     7501 tcctaacgct tcaataacca taccatcgat accttcacga ctataaaaac taaaaatgtc
     7561 acctggcata cccatatatg cttttactaa tggtacattt agtttgtcat tgacattttc
     7621 caatgcttgt tggcgatatg gcatatgatg gaattgcaca cgatcctttg tcaatacacc
     7681 tagcggacca tgatttggac tttgaaatgt gtttgtatta gacgtatgtg ttttggtaac
     7741 attacgcgcc gtatgaattt catcattaaa tacaaccatc acgcctttat gacgggcctt
     7801 ttcatcagag gcaacgcgaa tagcggaaat ataattatat aatccgtcag aaccaatttc
     7861 attagacgag cgcattgcgc cagtaataac aacaggttgc tcgataccta atattaaatc
     7921 aagtaaaaag gcagtttctt ctaacgtatc ggtaccatgc gtgataacga aaccatcata
     7981 atatttattt gttactgctt ctaaaataat atcctttaat tgtttaacat gttggattgt
     8041 catatgaggt gatggtacat taaaaggatt taattcatct atttgtgcat agtgatttat
     8101 gacatcttga tgcattgaaa tagggttgat atcatttgtt actactttat tagattggtc
     8161 ttgtgacata ctaatggtgc caccagtatg aataacaagt agatgtttca tatatttcct
     8221 cctatattta atttacctaa ttatgataaa atattattca taaaacgaca aggaagggaa
     8281 atgacgcatg aaagccatta atattgcatt agatggtcca gctgctgccg gaaaaagtac
     8341 aattgcgaaa cgtgtagcca gcgaactatc aatgatttat gtcgatacag gagcaatgta
     8401 tcgtgcatta acatacaaat atttaaaatt aaacaaaact gaggactttg caaaactagt
     8461 tgaccaaaca acattagatt taacttataa agcagataaa ggtcaatgtg tcattttaga
     8521 taacgaagat gtaacagact ttttaagaaa taatgatgtg acgcaacatg tttcatacgt
     8581 tgcatctaaa gagccagtac gttcattcgc cgttaaaaaa caaaaagagt tagctgcaga
     8641 aaaaggtatc gtaatggatg gtcgcgatat cggaactgta gtgctaccag atgcagattt
     8701 aaaagtatat atgattgcat cagttgaaga gcgagcagaa agaagatata aagataatca
     8761 attaagaggt atcgaatcaa attttgaaga tttaaaacgt gatattgaag ctcgtgatca
     8821 atatgacatg aaccgtgaaa tatcaccatt aagaaaagca gatgatgcag tgacattaga
     8881 tacgacaggc aagtcgattg aagaagttac tgacgaaatt ttagcgatgg tgagtcaaat
     8941 taaataatct aataagtcat aactatctat ttgtaatgtg caacgcttaa cgcatataca
     9001 aaatgaatgt gctgataatg atttactcaa attaaaaggt gatttttatt caatgatgaa
     9061 tgaaagttgc ctttttattt ttggtaaaag ttcatgcgtc agtgaattgt gtaagttttt
     9121 caaaaagtaa aaagaaataa taaaggtgaa ttattagaat tccaaaaata attcattaca
     9181 ttcataaagc attttacaaa tggtaagaaa atgagtgtta caaatctaaa tattgcaaaa
     9241 gaagttgatt tagtcacaaa aaatgtccta tgtaataatt cgagaaagat gcactatata
     9301 c
//
LOCUS       DANBWH010000106         9297 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.109, whole genome shotgun sequence.
ACCESSION   DANBWH010000106 DANBWH010000000
VERSION     DANBWH010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9297
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.109"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            14..682
                     /gene="capA"
                     /locus_tag="PPY92_001717"
     CDS             14..682
                     /gene="capA"
                     /locus_tag="PPY92_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     protein CapA"
                     /protein_id="HDJ2625710.1"
                     /translation="MESTLELTKIKEVLQKNLKILIILPLLFLIISAIVTFFVLSPKY
                     QANTQILVNQTKGDNPQFMAQEVQSNIQLVNTYKEIVKSPRILDEVSKDLNDKYSPSK
                     LSSMLTITNQENTQLINIQVKSGHKQDSEKIANSFAKVTSKQIPKIMSVDNVSILSKA
                     DGTAVKVAPKTVVNLIGAFFLGLVVALIYIFFKVIFDKRIKDEEDVEKELGLPVLGSI
                     QKFN"
     gene            698..1384
                     /gene="cap8B"
                     /locus_tag="PPY92_001718"
     CDS             698..1384
                     /gene="cap8B"
                     /locus_tag="PPY92_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8B"
                     /protein_id="HDJ2625711.1"
                     /translation="MSKKENTTTTLFVYEKPKSTISEKFRGIRSNIMFSKANGEVKRL
                     LVTSEKPGAGKSTVVSNVAITYAQAGYKTLVIDGDMRKPTQNYIFNEQNNNGLSSLII
                     GRTTMSEAITSTEIENLDLLTAGPVPPNPSELIGSERFKELVDLFNKRYDIIIVDTPP
                     VNTVTDAQLYARAIKDSLLVIDSEKNDKNEVKKAKALMEKAGSNILGVILNKTKVDKS
                     SSYYHYYGDE"
     gene            1387..2151
                     /gene="cap8C"
                     /locus_tag="PPY92_001719"
     CDS             1387..2151
                     /gene="cap8C"
                     /locus_tag="PPY92_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8C"
                     /protein_id="HDJ2625712.1"
                     /translation="MIDIHNHILPNIDDGPTNETEMMDLLKQATTQGVTEIIVTSHHL
                     HPRYTTPIEKVKSCLNHIESLEEVQALNLKFYYGQEIRITDQILNDIDRKVITGINDS
                     RYLLIEFPSNEVPHYTDQLFFELQSKGFVPIIAHPERNKAISQNLDILYDLINKGALS
                     QVTTASLAGISGKKIRKLAIQMIENNLTHFIGSDAHNTEIRPFLMKDLFNDKKLRDYY
                     EDMNGFISNAKLVVDDKKIPKRMPQQDYKQKRWFGL"
     gene            2171..3994
                     /gene="cap8D"
                     /locus_tag="PPY92_001720"
     CDS             2171..3994
                     /gene="cap8D"
                     /locus_tag="PPY92_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8D"
                     /protein_id="HDJ2625713.1"
                     /translation="MAHLSVKLRLLILALIDSLIVTFSVFVSYYILEPYFKTYSVKLL
                     ILAAISLFISHHISAFIFNMYHRAWEYASVSELILIVKAVTTSIVITMVVVTIVTGNR
                     PFFRLYLITWMMHLILIGGSRLFWRIYRKYLGGKSFNKKPTLVVGAGQAGSMLIRQML
                     KSDEMKLEPVLAVDDDEHKRNITITEGVKVQGKIADIPELVRKYKIKKIIIAIPTIGQ
                     ERLKEINNICHMDGVELLKMPNIEDVMSGELEVNQLKKVEVEDLLGRDPVELDMDMIS
                     NELTNKTILVTGAGGSIGSEICRQVCNFYPERIILLGHGENSIYLINRELRNRFGKNV
                     DIVPIIADVQNRARMFEIMETYKPYAVYHAAAHKHVPLMEDNPEEAVHNNILGTKNTA
                     EAAKNAEVKKFVMISTDKAVNPPNVMGASKRIAEMIIQSLNDETHRTNFVAVRFGNVL
                     GSRGSVIPLFKSQIEEGGPVTVTHPEMTRYFMTIPEASRLVLQAGALAEGGEVFVLDM
                     GEPVKIVDLARNLIKLSGKKEDDIRITYTGIRPGEKMFEELMNKDEVHPEQVFEKIYR
                     GKVQHMKCNEVEAIIQDIVNDFSKEKIINYANGKKGDNYVR"
     gene            3984..5012
                     /gene="cap8E"
                     /locus_tag="PPY92_001721"
     CDS             3984..5012
                     /gene="cap8E"
                     /locus_tag="PPY92_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8E"
                     /protein_id="HDJ2625714.1"
                     /translation="MFDDKILLITGGTGSFGNAVMKRFLDSNIKEIRIFSRDEKKQDD
                     IRKKYNNSKLKFYIGDVRDSQSVETAMRDVDYVFHAAALKQVPSCEFFPVEAVKTNII
                     GTENVLQSAIHQNVKKVICLSTDKAAYPINAMGISKAMMEKVFVAKSRNIRSEQTLIC
                     GTRYGNVMASRGSVIPLFIDKIKAGEPLTITDPDMTRFLMSLEDAVELVVHAFKHAET
                     GDIMVQKAPSSTVGDLATALLELFEADNAIEIIGTRHREKKAETLLTREEYAQCEDMG
                     DYFRVPADSRDLNYSNYVETGNEKITQSYEYNSDNTHILTVEEIKEKLLTLEYVRNEL
                     NDYKASMR"
     gene            5025..6134
                     /gene="cap8F"
                     /locus_tag="PPY92_001722"
     CDS             5025..6134
                     /gene="cap8F"
                     /locus_tag="PPY92_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8F"
                     /protein_id="HDJ2625715.1"
                     /translation="MNIVITGAKGFVGKNLKADLTSTTDHHIFEVHRQTKEEELESAL
                     LKADFIVHLAGVNRPEHDKEFSLGNVSYLDHVLDILTRNTKKPAILLSSSIQATQDNP
                     YGESKLQGEQLLREYAEEYGNTVYIYRWPNLFGKWCKPNYNSVIATFCYKIARNEEIQ
                     VNDRNVELTLNYVDDIVAEIKRAIEGTPTIENGVPTVPNVFKVTLGEIVDLLYKFKQS
                     RLDRTLPKLDNLFEKDLYSTYLSYLPSTDFSYPLLMNVDDRGSFTEFIKTPDRGQVSV
                     NISKPGITKGNHWHHTKNEKFLVVSGKGVIRFRHVNDDEIIEYYVSGDKLEVVDIPVG
                     YTHNIENLGDTDMVTIMWVNEMFDPNQPDTYFLEV"
     gene            6138..7262
                     /gene="cap8G"
                     /locus_tag="PPY92_001723"
     CDS             6138..7262
                     /gene="cap8G"
                     /locus_tag="PPY92_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8G"
                     /protein_id="HDJ2625716.1"
                     /translation="MEKLKLMTIVGTRPEIIRLSSTIKACDQYFNQILVHTGQNYDYT
                     LNQIFFDDLELRQPDHYLEAVGSNLGETMGNIIAKTYDVLLREQPDALLILGDTNSCL
                     AAVSAKRLKIPVFHMEAGNRCFDQNVPEEINRKIVDHVSDVNLPYTEHSRRYLLDEGF
                     NKANIFVTGSPMTEVIEAHRDKINHSDVLNKLGLEPQQYILVSAHREENIDNEKNFKS
                     LMNAINDIAKKYKMPVIYSTHPRSWKKIEESKFEFDPLVKQLKPFGFFDYNALQKDAF
                     VVLSDSGTLSEESSILKFPGVLIRTSTERPEVLDKGTVIVGGITYNNLIQSVELAREM
                     QNNNEPMIDAIDYKDTNVSTKVVKIIQSYKDIINRNTWRK"
     gene            7265..7891
                     /locus_tag="PPY92_001724"
     CDS             7265..7891
                     /locus_tag="PPY92_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HDJ2625717.1"
                     /translation="MRIAIEKIIGLLKNQSSKESNVKIHRLAYITNSKFDGNNYIDRW
                     CKIRNSHIGEYSYIGFGSDFNNVEVGRYCSISSDVKIGLGKHPTHFFSSSPIFYSNNN
                     PFNIKQKFIDFNDQPSRTTIKNDVWIGANVIIMDGLTINTGAVIAAGSVVTKNVGAYE
                     VVGGVPAKVIKKRFDNKTIEKLLESKWWEKTPDKLKGFSVEYLNKKDT"
     gene            7896..9005
                     /locus_tag="PPY92_001725"
     CDS             7896..9005
                     /locus_tag="PPY92_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDJ2625718.1"
                     /translation="MRILNIVSSNIVQDPRVLKQIETIKGVTNDYKIVGMNNSQATNR
                     RLENLDCNYRLLGSKVDPKNILSKLIKRIRFATGVIREIKAFKPDVIHANDFDVLLMV
                     YLSNYKKANIVYDAHEIYAKNAFINKVPLISKFVESIEKHIVKHRVNAFVTVSHAAKE
                     YYQSKGYKKEANVITNAPILNDSREFKEIENFKEIVYQGQIVMDRGYEEFIIASSAFK
                     QNAPSFIIRGFGPHEEVIKELISYNSENIRLDKPVEVKELVDKLAESNVGVILTKPVS
                     INFEYTVSNKIFECIHAGLPVILSPVKEHIYLNEKYKFGIVLKEVTPLEIEKAVRKLR
                     DNHDLFNHLRQNAIKASKILNWQIESERLVELYKF"
     gene            9019..>9297
                     /locus_tag="PPY92_001726"
     CDS             9019..>9297
                     /locus_tag="PPY92_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDJ2625719.1"
                     /translation="MKFFVLCAIISMNIFIVISTFTKEVLGFPIEPVYYSTMVGIALI
                     TTVFAIYKIIVTQEIPRGLILLIAICLLYLAFYYFSPDKEEKLAKNNIL"
BASE COUNT         3427 a         1268 c         1803 g         2799 t
ORIGIN      
        1 agggaggtaa ataatggaaa gtacattaga attaacaaaa attaaagaag tattacaaaa
       61 aaacttgaag attttaatta ttttaccgct attattttta attattagcg ctattgttac
      121 atttttcgtc ttatcaccta aatatcaagc taatactcaa atcttagtga atcaaactaa
      181 gggtgacaat cctcagttta tggcacaaga ggttcaaagt aatattcaac ttgtaaatac
      241 gtataaagaa attgttaaaa gtcctagaat tttagatgag gtgtcaaagg acttaaatga
      301 taagtattca ccatctaaat tgtcgagtat gttgacaatt acaaaccaag aaaatacgca
      361 acttatcaac atccaagtta aaagtggtca taaacaagat tcggaaaaaa ttgcgaatag
      421 cttcgctaaa gttacaagta aacaaattcc gaagattatg agtgtggata acgtatcaat
      481 tttatctaaa gcagacggta cagcagttaa agtcgcacca aaaactgtag tgaatctaat
      541 cggtgcattc tttttaggat tagttgtcgc gcttatatat atcttcttca aagtaatttt
      601 cgataagcga attaaagatg aagaagatgt agagaaagaa ttaggattgc ctgtattggg
      661 ttcaattcaa aaatttaatt aaggatggtt gctacttatg tcaaaaaagg aaaatacgac
      721 aacaacacta tttgtatatg aaaaaccaaa atcaacaatt agtgaaaagt ttcgaggtat
      781 acgttcaaac atcatgtttt caaaagcaaa tggtgaagta aagcgcttat tggttacttc
      841 tgaaaagcct ggtgcaggta aaagtacagt tgtatcgaat gtagcgatta cttatgcaca
      901 agcaggctat aagacattag ttattgatgg cgatatgcgt aagccaacac aaaactatat
      961 ttttaatgag caaaataata atggactatc aagcttaatc attggtcgaa cgactatgtc
     1021 agaagcaatt acgtcgacag aaattgaaaa tttagatttg ctaacagctg gccctgtacc
     1081 tccaaatcca tctgagttaa ttgggtctga aaggttcaaa gaattagttg atctgtttaa
     1141 taaacgttac gacattatta ttgtcgatac accgccagtt aatactgtga ctgatgcaca
     1201 actatatgcg cgtgctatta aagatagtct gttagtaatt gatagtgaaa aaaatgataa
     1261 aaatgaagtt aaaaaagcaa aagcacttat ggaaaaagca ggcagtaaca ttctaggtgt
     1321 cattttgaac aagacaaagg tcgataaatc ttctagttat tatcactatt atggagatga
     1381 ataagtatga ttgatattca taaccatata ttgcctaata tcgatgacgg tccgacaaat
     1441 gaaacagaga tgatggatct tttaaaacaa gcgacaacac aaggtgttac agaaatcatt
     1501 gtaacatcac atcacttaca tcctcgatat accacaccta tagaaaaagt gaaatcatgt
     1561 ttaaaccata ttgaaagctt agaggaagta caagcactaa atctaaagtt ttattatggt
     1621 caggaaataa gaattaccga tcaaatcctt aatgatattg atcgaaaagt tattaccggt
     1681 attaatgatt cacgctattt actaatagaa tttccatcaa atgaagttcc acactatact
     1741 gatcaattat ttttcgaatt acagagtaaa ggctttgtac cgattattgc acatccagag
     1801 cggaataaag caataagtca aaaccttgac atactatacg atttaattaa caaaggtgct
     1861 ttaagtcaag tgacaacggc gtcattagcg ggtatttccg gtaaaaaaat tagaaaatta
     1921 gcaattcaaa tgattgaaaa caatctgaca catttcatcg gttcagatgc gcataacaca
     1981 gaaatcagac cgttcttaat gaaagactta tttaatgata agaaattacg tgattattat
     2041 gaagatatga acggatttat tagtaatgcg aagttagttg ttgatgataa aaaaattcct
     2101 aaacgaatgc cacaacaaga ttataaacag aaaagatggt ttgggttata aacagcaaat
     2161 gaggggtttt atggcacatt tatctgtgaa attgcggctt ttaatactag cattaatcga
     2221 ttcactgata gtgacatttt cagtattcgt aagttattac attttagaac cgtatttcaa
     2281 aacatattct gtcaaattat taatattggc agctatatca ctattcatat cgcatcatat
     2341 ttcagcattt atttttaata tgtatcatcg agcgtgggaa tatgccagtg tgagtgaatt
     2401 gattttaatt gttaaagctg tgacgacatc tatcgttatt acgatggtgg tcgtgacaat
     2461 tgttacaggc aatagaccgt tttttagatt gtatttaatt acttggatga tgcacttgat
     2521 tttaataggt ggctcaaggt tattttggcg tatttatcgg aaataccttg gaggtaagtc
     2581 atttaataag aagccaactt tagttgttgg tgctggtcaa gcaggttcaa tgctgattag
     2641 acaaatgttg aaaagtgacg aaatgaaact tgaaccggta ttagcagtcg atgatgacga
     2701 acataaacgc aatatcacaa ttactgaggg tgtaaaagtc caaggtaaaa ttgcggatat
     2761 tccagaacta gtgaggaaat ataagattaa aaaaatcatc attgcaattc caactattgg
     2821 tcaagagcgt ttgaaagaaa ttaataatat ttgccatatg gatggcgttg agttattgaa
     2881 aatgccaaat atagaagacg tcatgtctgg tgagttagaa gtgaatcaac tgaaaaaagt
     2941 tgaagtagaa gatttactag gcagagatcc tgttgaatta gatatggata tgatatcaaa
     3001 tgaattgacg aataaaacta ttttagttac gggtgcaggt ggttcaatag gatcagaaat
     3061 ttgtagacaa gtttgtaatt tctatccaga acgtattatt ctacttggcc atggtgaaaa
     3121 cagtatttat ttaatcaatc gtgaattgcg aaatcgcttc ggaaaaaatg ttgatatcgt
     3181 tcctattata gcggatgtgc aaaatagagc gcgtatgttt gaaattatgg aaacgtataa
     3241 accatacgca gtttatcatg cagcagcaca caagcacgtg ccgttaatgg aagacaaccc
     3301 tgaagaagca gtacataata atattttagg tacgaaaaat actgctgaag ctgctaaaaa
     3361 tgcagaggta aagaaattcg ttatgatttc tacggataaa gccgttaatc cgcctaatgt
     3421 catgggagct tcaaagcgaa ttgcagaaat gattattcaa agtttaaatg atgaaacgca
     3481 tcgaacaaat tttgttgcag tgagatttgg taatgtactt ggatcgagag gatctgtgat
     3541 tccacttttc aaaagtcaaa ttgaagaagg tgggccagtt actgtgacac atcctgaaat
     3601 gacacgttac tttatgacaa ttcctgaagc ttctagacta gttttgcagg caggggcatt
     3661 agcagaaggt ggcgaagtat ttgtgctaga tatgggagaa ccagtgaaaa ttgtagattt
     3721 ggcacgtaat ttaattaagc taagtggtaa aaaagaagac gacatacgca ttacttatac
     3781 agggattaga cccggcgaaa aaatgtttga agagcttatg aataaagatg aggttcatcc
     3841 tgaacaagta tttgaaaaaa tttatcgtgg caaagtacaa catatgaaat gtaatgaagt
     3901 tgaagcgatt attcaagaca tcgtcaatga ctttagtaaa gaaaaaatta ttaactatgc
     3961 caatggcaaa aagggagata attatgttcg atgacaaaat tttattaatt actgggggca
     4021 caggatcatt cggtaatgct gttatgaaac ggtttttaga ttctaatatt aaagaaattc
     4081 gtattttttc acgcgatgag aaaaaacaag atgacattcg aaaaaaatat aataattcaa
     4141 aattaaagtt ctacattggt gatgtgcgtg atagtcaaag tgtagaaaca gcaatgcgag
     4201 atgttgatta cgtattccat gcagcagctt taaaacaagt gccgtcatgt gaattctttc
     4261 cagttgaggc agtgaagaca aatattattg gtacagaaaa tgtcttacaa agtgctattc
     4321 atcaaaatgt taaaaaagtc atatgtttat ctacagataa ggcagcgtat cctattaatg
     4381 ctatgggtat ttcaaaagca atgatggaaa aagtattcgt agccaaatca agaaatattc
     4441 gtagtgaaca aacgcttatt tgtggtacaa gatacggtaa tgtgatggct tcaagaggat
     4501 cagtaatacc tttgtttatc gacaaaatca aagctggaga acctttaacg attacagatc
     4561 ctgatatgac aagattttta atgagcttag aagatgcggt agaactagtt gttcatgcat
     4621 ttaagcatgc agagacagga gatattatgg ttcaaaaagc accaagctca acggtagggg
     4681 atcttgcgac cgcattatta gaattgtttg aagctgataa tgcaattgaa atcattggta
     4741 cgcgacatag agagaaaaaa gcagaaacat tgttgacgag agaagaatac gcacaatgtg
     4801 aagatatggg tgattatttt agagtgccgg cagactccag agatttaaat tatagtaatt
     4861 atgttgaaac cggtaacgaa aagattacgc aatcttatga atataactcc gataatacac
     4921 atattttaac ggtggaagag ataaaagaaa aacttttaac actagaatat gttagaaacg
     4981 aattgaatga ttataaagct tcaatgagat aggagagatt gacgttgaat attgtaatta
     5041 caggagcaaa aggttttgta ggaaaaaact tgaaagcaga tttaacatca acgacagatc
     5101 atcatatttt cgaagtacat cgacaaacta aagaggaaga attagagtca gcattgttga
     5161 aagcagactt tatcgtgcat ttagcgggtg ttaatcgacc tgaacatgac aaagaattca
     5221 gcttaggaaa cgtgagttat ttagatcatg tacttgatat attaactaga aatacgaaaa
     5281 agccagcgat attattatcg tcttcaatac aagcaacaca agataatcct tatggtgaga
     5341 gtaagttgca aggggaacag ctattaagag agtatgccga agagtatggc aatacggttt
     5401 atatttatcg ctggccaaat ttattcggca agtggtgtaa gccgaattat aactcagtga
     5461 tagcaacatt ttgttacaaa attgcacgta acgaagagat tcaagttaat gatcggaatg
     5521 ttgaactaac gctaaactac gtggatgata tcgtcgctga aataaagcgt gctattgaag
     5581 gaactccaac gattgaaaat ggtgtaccta cagtaccaaa cgtatttaaa gtgacattgg
     5641 gagaaattgt agatttatta tacaagttca aacagtcacg tctcgatcga acattgccga
     5701 aattagataa cttgtttgaa aaagatttgt atagtacgta tttaagctat ctacctagta
     5761 cagactttag ttatccctta cttatgaatg tggatgatag gggttctttt acagaattta
     5821 taaaaacacc ggatcgtggt caagtttctg taaatatttc taaaccaggt attactaaag
     5881 gtaatcactg gcaccatact aaaaacgaaa aatttctagt cgtatcaggt aaaggggtaa
     5941 ttcgttttag acatgttaat gatgatgaaa tcattgaata ttacgtttct ggcgataaat
     6001 tagaagttgt agacatacca gtaggataca cacataatat tgaaaattta ggcgacacag
     6061 atatggtaac tattatgtgg gtgaatgaaa tgtttgatcc aaatcagcca gatacgtatt
     6121 tcttggaggt atagcgcatg gaaaaactga aattaatgac aatagttggt acaaggcctg
     6181 aaatcattcg tttatcatca acgattaaag catgtgatca atattttaat cagatattag
     6241 tacacactgg tcaaaattat gattatacat tgaatcaaat tttctttgat gatttggaat
     6301 taagacaacc ggaccactac ttagaggcag ttggaagtaa ccttggagaa acgatgggga
     6361 atattattgc gaagacatat gacgttttat tacgcgaaca accagatgca cttttaattc
     6421 ttggtgatac aaatagttgt ttagcagcag tatctgctaa acgattaaag attcctgtgt
     6481 tccacatgga agcgggtaat agatgctttg atcagaatgt acctgaagaa atcaatcgta
     6541 aaattgttga ccatgtcagt gatgtgaatc taccttatac agaacatagc agacggtatt
     6601 tattagatga aggcttcaat aaagcgaata tctttgtgac aggatcaccg atgacagaag
     6661 tgatagaagc gcatcgagat aaaattaatc acagtgacgt tttaaataaa ctaggattag
     6721 aaccgcaaca atacatttta gtatctgcgc atagagaaga gaatatcgat aatgaaaaga
     6781 attttaaatc attaatgaat gcgataaatg atattgccaa aaagtataaa atgcctgtga
     6841 tttattcaac gcatccaaga agttggaaga aaattgaaga aagtaaattt gaatttgatc
     6901 cattagttaa acagttaaag ccatttggtt tctttgatta taatgcattg caaaaagatg
     6961 catttgttgt gctatcagat agtggaacat tgtcagaaga gtcgtctatt ttgaagttcc
     7021 ctggtgtcct tattcgaact tccacagaaa gaccggaagt actagataaa ggtacggtta
     7081 ttgtaggtgg tattacctat aacaatctaa tccaatccgt tgaactagca agagagatgc
     7141 aaaacaataa cgaaccgatg attgatgcta ttgattataa agacactaac gtttcgacaa
     7201 aggtagttaa aattattcaa agctataaag atattatcaa tcgaaatact tggaggaaat
     7261 gacgatgagg atagcgattg aaaagataat tggtttgctg aaaaaccagt cctctaaaga
     7321 atcgaatgtt aagattcatc gcttggcgta tattacaaac tcaaaatttg atggcaataa
     7381 ctatatagat agatggtgta aaatcaggaa ttctcacatt ggtgaataca gttatattgg
     7441 atttggtagt gattttaata atgtagaagt aggaagatat tgttcgatat cttcggatgt
     7501 aaaaattggg ttaggaaaac atcctacaca cttttttagc tcatcaccga ttttttattc
     7561 taataataat ccatttaaca taaagcaaaa gtttatagac tttaatgacc aaccaagccg
     7621 tacaacaatt aaaaatgatg tgtggattgg tgcaaatgta attattatgg atggattaac
     7681 aataaatact ggtgcagtca tagcagccgg ctcagttgtt actaaaaatg taggagcata
     7741 tgaggttgtt ggtggggttc ctgcaaaagt gattaagaag cgatttgaca ataaaacaat
     7801 tgaaaaactt ttggaaagca agtggtggga gaaaacgcct gacaaactaa aaggattttc
     7861 ggttgaatat ttaaataaaa aggatactta atgatatgag aattttaaat attgtatcga
     7921 gtaatattgt tcaagaccca agggtactta aacaaataga aacaattaaa ggcgttacga
     7981 atgattataa aattgttgga atgaataatt cacaagctac taataggcga ttggaaaatt
     8041 tagattgtaa ttatcgtttg ttaggtagca aggtagatcc caaaaatatt ctttctaaat
     8101 taattaagcg tataagattt gcaacaggtg ttatccgaga aattaaagct tttaaacctg
     8161 acgtgattca tgcaaatgat ttcgacgtat tattaatggt ctatttaagc aattataaaa
     8221 aagctaatat tgtttatgat gcgcatgaaa tatatgcgaa aaatgccttt attaataaag
     8281 ttccacttat ttcaaagttt gtagaaagta tagaaaaaca catagtaaaa catcgtgtta
     8341 atgccttcgt aacagtaagt catgcagcaa aagaatatta tcaatctaaa ggatataaga
     8401 aggaagcgaa tgttattacg aatgcaccta ttttaaatga tagcagagaa tttaaagaaa
     8461 tcgaaaactt taaagaaatc gtatatcaag gtcaaattgt aatggacaga ggatatgaag
     8521 agtttattat tgcttcatca gcttttaaac aaaatgctcc ttcattcata attcgagggt
     8581 ttggtccgca tgaagaagtg ataaaagaac tgattagtta taactcggaa aatattaggt
     8641 tggataaacc agttgaagta aaagaattgg ttgataagtt agcagaaagt aatgttggtg
     8701 ttatcttgac gaaacctgta tctattaatt ttgaatatac agtatctaat aaaatttttg
     8761 aatgtataca tgctggttta ccagtaattt tatctcctgt caaagagcat atttatctca
     8821 atgaaaaata taaatttggc attgttttaa aggaagttac gccgttagaa attgaaaagg
     8881 cggttagaaa attaagagat aatcacgatt tgtttaatca tttacgtcaa aatgcaatta
     8941 aggcgtctaa aattttgaat tggcaaatag aaagtgaacg attagtagaa ttatataaat
     9001 tttaaagaga ggtaaactat gaaatttttt gtactttgtg caattatcag catgaacata
     9061 tttatagtaa tctctacatt tactaaagaa gtattagggt tccctataga gccggtgtat
     9121 tactcaacca tggttggtat agcattaatt actacggtgt ttgctattta taagataatt
     9181 gtcacgcaag aaattccgcg agggttaata ttattaattg ctatatgttt gctttatcta
     9241 gctttttatt atttttcacc agataaggaa gagaaactag ctaaaaataa tattcta
//
LOCUS       DANBWH010000107         9288 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.110, whole genome shotgun sequence.
ACCESSION   DANBWH010000107 DANBWH010000000
VERSION     DANBWH010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9288
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.110"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(247..2703)
                     /locus_tag="PPY92_001727"
     CDS             complement(247..2703)
                     /locus_tag="PPY92_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDJ2625720.1"
                     /translation="MLFGRLTERAQRVLAHAQEEAIRLNHSNIGTEHLLLGLMKEPEG
                     IAAKVLESFNITEDKVIEEVEKLIGHGQDHVGTLHYTPRAKKVIELSMDEARKLHHNF
                     VGTEHILLGLIRENEGVAARVFANLDLNITKARAQVVKALGNPEMSNKNAQASKSNNT
                     PTLDSLARDLTVIAKDGTLDPVIGRDKEITRVIEVLSRRTKNNPVLIGEPGVGKTAIA
                     EGLAQAIVNNEVPETLKDKRVMSLDMGTVVAGTKYRGEFEERLKKVMEEIQQAGNVIL
                     FIDELHTLVGAGGAEGAIDASNILKPALARGELQCIGATTLDEYRKNIEKDAALERRF
                     QPVQVDEPSVVDTVAILKGLRDRYEAHHRINISDEAIEAAVKLSNRYVSDRFLPDKAI
                     DLIDEASSKVRLKSHTTPNNLKEIEQEIEKVKNEKDAAVHAQEFENAANLRDKQTKLE
                     KQYEEAKNEWKNAQNGMSTSLSEEDIAEVIAGWTGIPLTKINETESEKLLSLEDTLHE
                     RVIGQKDAVNSISKAVRRARAGLKDPKRPIGSFIFLGPTGVGKTELARALAESMFGDD
                     DAMIRVDMSEFMEKHAVSRLVGAPPGYVGHDDGGQLTEKVRRKPYSVILFDEIEKAHP
                     DVFNILLQVLDDGHLTDTKGRTVDFRNTIIIMTSNVGAQELQDQRFAGFGGSSDGQDY
                     ETIRKTMLKELKNSFRPEFLNRVDDIIVFHKLTKEELKEIVTMMVNKLTNRLSEQNIN
                     IIVTDKAKDKIAEEGYDPEYGARPLIRAIQKTIEDNLSELILDGNQIEGKKVTVDHDG
                     KEFKYDIAEQTSETKTPSQA"
     gene            complement(2717..3724)
                     /locus_tag="PPY92_001728"
     CDS             complement(2717..3724)
                     /locus_tag="PPY92_001728"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HDJ2625721.1"
                     /translation="MTHNIHDNISQWMKSNEETPIVMSSRIRLARNLENHVHPLMYAT
                     ENDGFRVINEVQDALPNFELMRLDQMDQQSKMKMVAKHLISPELIKQPAAAVLVNDDE
                     SLSVMINEEDHIRIQAMGTDTTLQALYNQASSIDDELDRSLDISYDEQLGYLTTCPTN
                     IGTGMRASVMLHLPGLSIMKRMTRIAQTINRFGYTIRGIYGEGSQVYGHTYQVSNQLT
                     LGKSELEIIETLTEVVNQIIHDEKQIRQKLDTYNQLETQDRVFRSLGILQNCRMITME
                     EASYRLSEVKLGIDLNYIELQNFKFNELMVAIQSPFLLDEEDDKSVKEKRADILREHI
                     K"
     gene            complement(3714..4280)
                     /locus_tag="PPY92_001729"
     CDS             complement(3714..4280)
                     /locus_tag="PPY92_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HDJ2625722.1"
                     /translation="MLCENCQLNEAELKVKVTSKNKTEEKMVCQTCAEGHHPWNQANE
                     QPEYQEHQDNFEEAFVVKQILQHLATKHGINFQEVAFKEEKRCPSCHMTLKDIAHVGK
                     FGCANCYATFKDDIIDIVRRVQGGQFEHVGKTPHSSHKKIALKRKIEEKNEYLKKLIE
                     IQDFEEAAIVRDEIKALKAESEVQHDDA"
     gene            complement(4299..4760)
                     /locus_tag="PPY92_001730"
     CDS             complement(4299..4760)
                     /locus_tag="PPY92_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDJ2625723.1"
                     /translation="MHNMSDIIEQYIKRLFEESNEDVVEIQRANIAQRFDCVPSQLNY
                     VIKTRFTNEHGYEIESKRGGGGYIRITKIENKDATGYINHLLQLIGPSISQQQAYYII
                     DGLLDKMLINEREAKMIQAVIDRETLSMDMVSRDIIRANILKRLLPVINYY"
     gene            4918..6132
                     /locus_tag="PPY92_001731"
     CDS             4918..6132
                     /locus_tag="PPY92_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDJ2625724.1"
                     /translation="MHILIGIIGIIFFLALAFLFSSDRKNIRWKYVGILLAIQLIFAF
                     ILLKTTMGIKVIGGISNGFNYLLLKAADGVNFVFGGIQYIDPKQPPFFFSVLLPIVFI
                     SAIIGILQYTKILPLIINVLGFLISKINGMGRLESYNAVAAAILGQSEVFISIKKQLP
                     YIPKQRLYTLTASAMSTVSASIIGAYFTLIEPKYVVTAVVLNLFGGFIIASIINPYKV
                     NEEDDKLLVEETEERQQSFFEMLGEYILDGFKVAVIVGAMLIGYIAIIALLNGIVSNI
                     FSTVSGGAISWDFQTLIGFVFAPFAFLVGVPWQDAVQAGSVMATKLLSNEFVAMQALG
                     KLSDLSEHAKGVTSVFLVSFANFSSIGIISGAIKSLNDKKGDTVARFGLKLLFGATLV
                     SFISAAIAGFFI"
     gene            complement(6340..6900)
                     /gene="pdxT"
                     /locus_tag="PPY92_001732"
     CDS             complement(6340..6900)
                     /gene="pdxT"
                     /locus_tag="PPY92_001732"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HDJ2625725.1"
                     /translation="MKIGVLALQGAVREHIRHIELSGHEGIAVKKVEQLEEIEGLILP
                     GGESTTLRRLMNLYGFKEALQNSTLPMFGTCAGLIVLAQDIVGEEGYLNKLNITVQRN
                     SFGRQVDSFETELDIKGIATDIEGVFIRAPHIEKVGQGVDILCKVNEKIVAVQQGKYL
                     GVSFHPELTDDYRVTDYFINHIVKKA"
     gene            complement(6904..7791)
                     /gene="pdxS"
                     /locus_tag="PPY92_001733"
     CDS             complement(6904..7791)
                     /gene="pdxS"
                     /locus_tag="PPY92_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HDJ2625726.1"
                     /translation="MSKIIGSDRVKRGMAEMQKGGVIMDVVNAEQARIAEEAGAVAVM
                     ALERVPSDIRAAGGVARMANPKIVEEVMNAVSIPVMAKARIGHITEARVLEAMGVDYI
                     DESEVLTPADEEYHLRKDQFTVPFVCGCRNLGEAARRIGEGAAMLRTKGEPGTGNIVE
                     AVRHMRQVNSEVSRLTVMNDDEIMTFAKDIGAPYEILKQIKDNGRLPVVNFAAGGVAT
                     PQDAALMMELGADGVFVGSGIFKSEDPEKFAKAIVQATTHYQDYELIGRLASELGTAM
                     KGLDINQLSLEERMQERGW"
     gene            7895..9277
                     /locus_tag="PPY92_001734"
     CDS             7895..9277
                     /locus_tag="PPY92_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDJ2625727.1"
                     /translation="MKNTLYHQLYEKLKKQIIEGQFKEGDKFYSKRQLSKHLSISQTT
                     VEHAYQLLLDEGYIYSRPRSGYFVSEIESLTILNNQPIPSLFDDDSYKPKASDEAYDY
                     AFNLDEIDTKHFPIELFRKYSKDLYDTNHLNQLRRGHFQGELHLRFQLAFYLFTNRGV
                     ICDPNQIIIGSSTEQLVNQLVDLLYTSTFIIEKPSYPPIKNILDKKQVEYEQIEVEDN
                     GINVDEVIKSQKNIVYITPSHQFPTGYVMDLKKRTQLIQWAQEKEERFIIEDDYDSEF
                     RYFGKPIPAIQGLYSRGEKVIYISTFSKSIFPSCRVAYMVLPYSIMKKYHSQNHIEGN
                     TVPVHMQNLIATFISSGGFERHLNKMRRIYRRKLTYILKRLKPYKEQLDIQGAETGMH
                     FTITVKNGLTLQECLDRANKVKLKLQVYNFDDDQDYKKTPKFILGFGGIDDDALKPHV
                     DALIKSLIIK"
BASE COUNT         2859 a         1732 c         1302 g         3395 t
ORIGIN      
        1 atattatata gacctatttt attttgacgt aagttgctag tatcttcaaa caaaaaacct
       61 ttataaaaat tcataccttt atgctatcgc tgtaggctca ttaacttgtt acatataatt
      121 cttaactatc ctttgatgat tgttttatta gattgtttcg ttgatggata ctttcacgaa
      181 tttctatagt tcaatgctac taaaaaaaca gcccaaaact ttaatttgtt ttggactgtt
      241 ttataattat gcttgcgatg gtgttttagt ttctgaagtt tgttcagcaa tgtcatattt
      301 aaactcttta ccatcatgat ctactgtaac tttcttacct tcaatttgat taccatctaa
      361 tattaattca cttaaattat cttcgatagt tttttgtatc gctctaatta atggtcttgc
      421 accatattct ggatcatatc cttcttctgc gattttgtct ttcgctttat cagtaacaat
      481 aatatttatg ttttgttcag ataatcgatt tgttaattta ttaaccatca ttgttacaat
      541 ttcttttaat tcttcttttg ttagtttatg gaatacaatg atatcatcta cacggtttaa
      601 aaattctgga cggaatgaat tttttaattc ttttaacatc gtttttcgaa ttgtttcata
      661 atcttgtcca tcacttgaac caccgaatcc agcaaatcgt tgatcttgta attcttgtgc
      721 cccaacgttt gatgtcatta tgataattgt atttctgaaa tcaactgtac gtccttttgt
      781 atctgtcaaa tgtccatcat ctaaaacttg taatagaata ttaaatacat ctggatgagc
      841 tttttcaatt tcatcaaata aaattacaga atatggttta cgtctaactt tttcagttaa
      901 ttgtccacca tcatcatgac caacatatcc tggaggagca ccaactaatc ggctcactgc
      961 gtgtttttcc ataaattcac tcatgtctac acggatcatc gcatcatcat cgccaaacat
     1021 tgattcagct aaagctctag ctaattcagt tttaccaaca ccagttggtc caaggaagat
     1081 aaagctacca attggtcgtt taggatcttt taaccctgca cgggcacgtc taaccgcttt
     1141 actgattgaa ttaacagcat ctttttgccc aataactctc tcatgtaatg tatcttctag
     1201 actaagaagt ttttcagatt ctgtttcatt gattttagtt aatgggatac ctgtccatcc
     1261 tgcaataact tcagcaatat cttcttctga caatgaagtt gacatgccat tttgtgcatt
     1321 cttccattca tttttagctt cttcatattg cttttcaagt tttgtttgtt tatcacgcag
     1381 gttagcagca ttttcaaact cttgagcatg tactgcggca tctttttcat ttttaacttt
     1441 ttcaatttct tgttcaattt cttttaaatt attaggtgtc gtatgactct taagtcttac
     1501 tttagaactt gcttcatcaa ttaaatcaat tgctttatct ggtaagaaac gatctgaaac
     1561 gtatctgtta cttaatttaa cagctgcttc aatagcttcg tctgaaatat taatacgatg
     1621 gtgtgcttcg taacgatctc ttaatccttt taaaatagca actgtatcta ctactgaagg
     1681 ttcatcaact tgtacaggtt ggaaacgacg ttctaaagcc gcgtcttttt caatattttt
     1741 gcgatattca tctaatgtag tagcaccaat acattgtaat tcaccacgtg ctaatgccgg
     1801 tttcaaaata ttcgaagcat cgatagcacc ttcagcacca ccagcaccaa ctaaagtatg
     1861 caactcatca ataaatagga tgacattacc tgcttgttgg atttcttcca taaccttttt
     1921 cagacgctct tcaaattcac cacgatattt agtacctgca actactgttc ccatatctaa
     1981 agacataaca cgcttatctt ttaatgtctc tggtacctca ttattcacta tggcttgcgc
     2041 taaaccttca gcaatagcag ttttaccaac acctggctct ccaataagta caggattgtt
     2101 tttcgtacgt ctacttaata cttcaattac acgtgtaatt tctttatcac gtcctataac
     2161 aggatctaat gtaccgtctt tggcaatgac tgttaagtca cgagctaaac tatctaaagt
     2221 tggagtatta tttgacttac tagcttgtgc atttttatta ctcatttcag ggtttcctaa
     2281 agctttcaca acttgtgcac gtgctttagt aatatttaaa tctagatttg caaaaactct
     2341 tgctgcaaca ccttcatttt cacgaatcaa gcctaataaa atatgttccg ttccaacaaa
     2401 attgtgatgt aattttctag cttcatccat cgataattca atgacttttt tagctctagg
     2461 tgtataatgc aatgtaccaa catgatcttg accatgtccg attaattttt caacttcttc
     2521 aattacttta tcttcagtga tattaaaact ttctaatact tttgcagcaa ttccttcagg
     2581 ttctttcatt aaccccaata ataggtgttc tgttcctata tttgaatgat ttaaacgaat
     2641 tgcttcttct tgtgcatgtg ctaatacgcg ctgtgcacgt tcagttaatc taccaaataa
     2701 cataaataat gacctcctac tttatatgtt ctcttagtat atctgctcgt ttttctttta
     2761 cagatttgtc atcttcttca tctaataaaa atggtgactg tatagctacc atcaattcat
     2821 taaatttaaa gttttgtaat tcaatgtaat ttaaatctat accaagttta acttcgctta
     2881 atctataaga agcctcttcc atagttatca ttctacagtt ttgtaaaata cctagcgagc
     2941 gaaaaacacg gtcttgtgtt tctaattgat tataagtgtc taacttttgt cgtatttgtt
     3001 tttcatcatg aatgatttga ttaacaactt ctgttaatgt ttctatgatt tctaactcag
     3061 atttaccaag tgtaagttgg ttggatactt gataagtatg tccataaact tgcgaacctt
     3121 caccgtaaat acctctgatt gtatatccaa aacgattaat ggtttgagca atccgtgtca
     3181 ttcttttcat aatagataga cctggtaaat gtagcatcac gcttgctctc ataccagtac
     3241 ctatattggt aggacatgta gttaaataac caagttgttc atcataactt atatcaaggc
     3301 ttcgatctaa ttcatcatca attgatgaag cttgattata taaagcctgt aatgtcgtgt
     3361 cagttcccat agcttgaata cgaatatggt cctcttcatt tatcatgaca cttaaagatt
     3421 catcatcatt cactaatact gcggctgctg gttgttttat tagttcagga ctaatcaaat
     3481 gctttgcaac cattttcatt ttactttgtt gatccatttg atcaagacgc attaattcaa
     3541 aatttggcaa ggcatcttgt acctcattta taactctaaa tccatcattt tcagtagcat
     3601 acattagtgg atgcacatga ttttctaaat tacgcgctaa ccgaattcta gaagacataa
     3661 caattggtgt ttcttcatta cttttcatcc attggctgat attatcatga atattatgcg
     3721 tcatcatgtt gcacctcact ctcagctttt agtgctttaa tttcatctct aacaatggct
     3781 gcttcctcaa aatcttggat ttcaataagt tttttcaaat attcattctt ttcttcgatt
     3841 tttcgcttta aagctatctt tttatgtgaa gaatgtggtg tctttccaac gtgctcaaat
     3901 tgtccacctt gaactctgcg gacgatatca atgatgtcat ctttaaatgt tgcataacaa
     3961 ttagcacacc caaatttacc aacatgtgca atatctttca aagtcatatg acatgatggg
     4021 caacgttttt cttctttaaa cgctacttct tgaaaattaa tgccatgttt cgtagctaaa
     4081 tgttgtaaaa tttgcttaac aacaaatgct tcttcgaaat tatcttgatg ttcttgatat
     4141 tcaggctgtt cattagcttg attccacgga tggtgcccct cagcacaagt ttgacacacc
     4201 attttttctt ctgttttatt tttacttgta actttaactt ttaattccgc ttcattaagt
     4261 tgacaatttt cacaaagcac ttcaacacct catttcattt agtaataatt tataactggt
     4321 aacaaacgtt ttaaaatatt tgctctaata atatctctag aaaccatatc cattgatagc
     4381 gtttctctat caataactgc ttgaatcatt ttagcttcac gttcatttat taacatttta
     4441 tctaaaagcc catcaataat ataataagct tgttgttgag aaatagaagg tccaatcagc
     4501 tgaagcaaat gattaatata acctgttgca tctttatttt caattttagt gattcggatg
     4561 taaccaccac caccacgttt actttcgatt tcataaccat gttcattagt gaatcgtgtt
     4621 ttgattacat aatttaattg tgatggtaca caatcaaaac gctgtgcgat attcgctctc
     4681 tgaatttcaa cgacatcttc attcgactct tcaaataaac gtttgatgta ttgttctatg
     4741 atgtcagaca tattgtgcat gtatatcacc cctttttgac cttctttgac tatattatat
     4801 gacctatttt gacctttttc aattaatttg tttttatatt ttcaaaaatt tatgatgtta
     4861 gcccttccat tttattgtat gatagattgt agaaaaaact aaaggaatga gaaatatatg
     4921 catattctaa ttgggattat cggaattatc ttctttttag cacttgcgtt tctatttagc
     4981 tcagacagga aaaatattcg ctggaaatat gtaggaattt tacttgcaat tcagcttatc
     5041 tttgcattta tcttgcttaa aactacaatg ggaatcaagg ttattggtgg tatttcaaac
     5101 ggttttaact acttattatt aaaagcagca gatggtgtta actttgtatt cggtggtatt
     5161 caatatatcg atcctaagca accaccattc ttcttcagtg tattattacc tatcgtattt
     5221 atttcagcga ttatcggtat ccttcaatac acaaagattt tacctttaat cattaatgtt
     5281 ttaggattct taatttctaa aattaatggt atgggacgtt tagaatcata caatgcagtt
     5341 gctgcagcaa ttttaggaca atctgaagta tttatttcaa ttaaaaaaca attaccatac
     5401 atacctaagc aacgtttata cacattaaca gcttcagcaa tgtcgactgt ttctgcttca
     5461 attatcggtg catactttac cttaattgaa cctaaatatg ttgtaacggc tgttgtctta
     5521 aacttatttg gtggtttcat catcgcttca attattaacc cttataaagt taatgaagaa
     5581 gatgacaaat tattagttga agaaactgaa gaaagacaac aatcattctt cgaaatgcta
     5641 ggagaatata ttttagatgg atttaaagta gctgtcattg taggtgcaat gttaatcgga
     5701 tacattgcca ttatcgctct attaaatgga attgtaagca atatctttag tacagtttca
     5761 ggtggcgcta tttcttggga tttccaaaca ttaattggtt ttgtctttgc accatttgca
     5821 ttcttagtcg gtgtgccatg gcaagatgca gttcaagccg gttcagttat ggcgacaaaa
     5881 ctattatcta acgaatttgt agcaatgcaa gcacttggta aacttagtga tttatctgaa
     5941 cacgcaaaag gtgttacatc tgtattctta gtatcatttg ctaactttag ctctattggt
     6001 attatttcag gtgctattaa atcacttaat gacaaaaaag gagatacagt tgcccgcttt
     6061 ggtttgaaat tattattcgg tgcaacacta gtatctttca tttcagctgc aatcgctgga
     6121 ttctttattt aacaaataca tcaacatcga attaatccct tcaaaatttt gactattctc
     6181 agtctctttg aaggggtttt ttcttatgta tatctttatt ttttatcatt tttaattttg
     6241 acataaaaaa taactcctcc aaagctaagt ttgaaggaga tattttcttc tcaacaaatc
     6301 tataaatatc aataattcgt tgatttagca tacattaagc tatgcttttt ttacaatatg
     6361 attaataaag taatcagtta ctctatagtc atctgttaat tcaggatgga atgatacgcc
     6421 taaatattta ccttgctgga cagctacaat tttctcatta accttacata ggatatctac
     6481 gccttgacct actttttcaa tatgtggcgc tcttataaag acaccttcaa tatctgtagc
     6541 gataccttta atatctaatt ctgtttcaaa gctgtcaact tgtctaccga atgagtttcg
     6601 ttgtacagta atattcaact tgttaaggta tccttcttca ccaactatat cttgcgctag
     6661 aactattaat cctgcgcatg taccaaacat aggtaaagtt gaattttgta aagcctcttt
     6721 aaatccatat aaattcatta atcgacgtaa cgttgtagac tcgccaccag gtaatattaa
     6781 gccctcgatt tcttctaatt gttcaacttt tttaactgca ataccttcat gaccacttaa
     6841 ttcaatatgt ctaatatgtt cacgtactgc accttgtaat gctaatacac ctattttcat
     6901 atcttaccaa ccacgctctt gcatacgttc ttctaatgat aattgattga tatctaaacc
     6961 tttcatagca gtgccaagtt cacttgctaa tcttccaatt agttcatagt cttggtaatg
     7021 tgttgttgct tgaacaattg ctttagcaaa tttttctgga tcttctgatt taaaaatacc
     7081 tgatccaacg aatacaccgt cagcacctaa ttccatcatt aaagcagcat cttgaggagt
     7141 cgcaacgcca ccagctgcaa agttaactac cggtaaacga ccattgtctt taatttgttt
     7201 taaaatttca taaggcgcac cgatatcttt cgcaaaagtc ataatctcat catcattcat
     7261 tacagtcaat cgactaactt ctgaattaac ttgtctcata tgtcttacag cttcaacaat
     7321 attacctgta cctggttcac ctttagtacg taacatagca gcaccttcac caattctacg
     7381 cgcagcttca cctaaattac gacatccaca tacaaatggt actgtaaatt gatcttttct
     7441 taagtgatat tcctcatctg ctggtgttaa cacttctgat tcatcaatat agtcaacacc
     7501 catcgcctct aatactcttg cttcagtgat atgaccaata cgtgctttag ccatgactgg
     7561 aatagaaaca gcattcatta cttcttctac aattttaggg tttgccatac gtgcaacgcc
     7621 accagcagct ctaatatcag aaggtactcg ttctaatgcc ataactgcta ccgcgcctgc
     7681 ttcttctgca attcttgctt gctcagcatt aacgacatcc ataataacgc cgcctttttg
     7741 catttcagcc atacctcttt tgactctgtc tgatccaata attttactca ttatacataa
     7801 aacccctttt caagttgatt aaatacattg taatgtataa actgatattt aaaaaggtac
     7861 aaaataagat aaatttagga ggtcagttat aaaattgaaa aacactttat accatcaact
     7921 ttatgaaaaa ttaaaaaaac aaataattga aggtcaattt aaagaaggcg ataaatttta
     7981 ttctaaaaga caattaagca aacatttatc tatcagccaa acaaccgtag agcatgcata
     8041 tcaacttcta ttagacgaag gttacattta ttcaagacct cgctctggat attttgtgtc
     8101 cgaaattgaa tctttaacaa ttttaaacaa tcaacctatc ccctctcttt ttgatgatga
     8161 ttcatataaa ccaaaagcga gtgatgaagc ttatgattat gcttttaatt tggatgaaat
     8221 agatacgaaa catttcccta tagaactttt cagaaagtat tccaaagatc tttatgatac
     8281 aaatcattta aatcaattac gtagaggaca ttttcaagga gaattacatt taagatttca
     8341 actggcattt tatttattta ctaatcgtgg tgtaatttgt gatcctaatc aaattataat
     8401 tggctcatct accgaacaat tagtaaatca attagtcgat ttactttata cttcaacgtt
     8461 tattatcgaa aaacctagtt atccaccaat caaaaatatt ttagataaaa aacaagttga
     8521 atatgaacaa attgaagttg aagataatgg aataaacgta gatgaagtta ttaaatcaca
     8581 aaaaaatata gtatatataa cgccttccca tcaatttcca actggatatg tgatggactt
     8641 aaaaaaacga actcaattaa tacaatgggc tcaagaaaaa gaagaacgat ttattataga
     8701 agatgattat gattccgagt ttagatattt cggcaaaccc attccagcaa ttcaaggtct
     8761 atattcaaga ggagaaaaag tgatttatat cagtacattt tcaaagtcta tttttcctag
     8821 ctgtcgagtt gcatatatgg tgttacccta ttctataatg aaaaaatatc attctcaaaa
     8881 tcacattgaa ggcaacactg tacctgttca tatgcaaaac cttattgcaa cgtttatatc
     8941 ttccggtggt tttgaaaggc atttaaataa aatgagacga atttatcgta gaaaactaac
     9001 ctatatttta aaaagattga aaccttacaa agagcagctt gatattcaag gtgctgaaac
     9061 gggtatgcat tttacaatta cagtaaaaaa tggattaaca ttacaagagt gtcttgatag
     9121 agcaaacaaa gtaaagttaa aattacaagt ttataatttt gatgatgatc aagactataa
     9181 gaaaacccct aaatttatat taggctttgg cggtatagac gatgatgctt taaaaccgca
     9241 tgtagacgct ctaataaaat cattaattat aaagtaacac ttctttga
//
LOCUS       DANBWH010000108         9242 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.111, whole genome shotgun sequence.
ACCESSION   DANBWH010000108 DANBWH010000000
VERSION     DANBWH010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9242
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.111"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            12..164
                     /locus_tag="PPY92_001735"
     CDS             12..164
                     /locus_tag="PPY92_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001802730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625728.1"
                     /translation="MIDRLYASTFFVDCLTVDSHLYTLTNFENLNHYLIILLIAHAQH
                     KKETCQ"
     gene            161..2590
                     /locus_tag="PPY92_001736"
     CDS             161..2590
                     /locus_tag="PPY92_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase 8 family protein"
                     /protein_id="HDJ2625729.1"
                     /translation="MTYRMKKWQKLSTITLLMAGVITLNGGEFRSIDKHQIAVADTNV
                     QTPDYEKLRNTWLDVNYGYDKYDEKNDAMKKKFDATEKEAEKLLSSMKTESGRTYLWD
                     SAKDLDNKSADMTRTYRNIEKIAEAMRHPKTTLNTDENKKKVKDALEWLHKNAYGKEP
                     DNKVKELTENFKITDSSKKKALNWWDYEIGTPRSLTNTLILLNDQFSNEEKKKFTAPI
                     KTFAPKSDEILSSVGKAEPAKGGNLVDIAKVKLLESIIEEDKDMMKNSIDSFNKVFTY
                     VQDSATDKERNGFYKDGSYIDHKDVPYTGAYGVVLLEGISQMMPMIKETPFNDKTQNN
                     TTLKSWIDDGFLPLIYKGEMMDLSRGRAISRENETSHSASATVMKSLLRLSDAMDDST
                     KAKYKKIVKTSVKSDSSYGQNDTLSSYSDISKMKSLMEDSTISTNGLTQQLKIYNDMD
                     RVTYHNKDLDFAFGLSMTSKNVARYESINGENLKGWHTGAGMSYLYNSDVKHYRDNFW
                     ATADMKRLAGTTTLENEEPKGTDVKKSSKTFVGGTKFDDQHASIGMDFENQDKTLTAK
                     KSYFILNDKIVFLGTGIKSTDSSKNPVTTIENRKANGYTLYTDDKQTTASDNQGTNSV
                     FLESTNKPKNNIGYHFLNESKITVKKESHTGKWSDINKSQKQDSKTNQYYEVTQKHSN
                     TDSKYAYVLYPGLSKDDFNTKKDKVTVVKQDDDFHVVKDNESVWAGVNYSNSTQTFDI
                     NNTKVEVKAKGMFILKNKDDNTYECSFYNPESTNTASDIESKISITGYSITNKNTSTS
                     NESGVHFELTK"
     gene            complement(2650..3504)
                     /locus_tag="PPY92_001737"
     CDS             complement(2650..3504)
                     /locus_tag="PPY92_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HDJ2625730.1"
                     /translation="MNKTNFGDYLNKSKSRKIYKIFDQNLKREFSYWQFRKLFKSYTR
                     QSDVHELKVCTQNDDTMEQIWIDKANQFGVAVTWKDNSIIGFVMRPLINYDAIKNETT
                     LKYAVPTTDCYEVFWGGDNELYNYHYLYLHQRYAIDLVIKENHKTYKNQGKVNTDYFC
                     FGKDIIAPANGTVEKVVNGVQDNSIGSTNESQFLGNYIVIKHAENEYSLIAHLQQYSI
                     IVNEGQNVKYGDFLGKVGNSGNSTEPHIHFQVMNDKNIEACTSLKIRFLNNLELIKGD
                     VVCGLQGE"
     gene            complement(3715..4530)
                     /locus_tag="PPY92_001738"
     CDS             complement(3715..4530)
                     /locus_tag="PPY92_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HDJ2625731.1"
                     /translation="MSKRLLLFDFDETYFKHNTNEEDLSHLREMEKLLEKLTNNNEVI
                     TAVLTGSTFQSVMDKMDQVNMTFKPLHIFSDLSSKMFTWNNGEYVESETYKKKVLSEP
                     FLFEDIEDILRHISAQYNVEFIPQRAFEGNETHYNFYFHSTGNHNNDSRILEALVRYA
                     NDQNYTARFSRSNPLAGDPENAYDIDFTPSNAGKLYATQFLMKKYNIPVKSILGFGDS
                     GNDEAYLSYLEHAYLMSNSRDEALKQKFRLTKYPYYQGITLHVKEFVEGKYDY"
     gene            4825..5250
                     /locus_tag="PPY92_001739"
     CDS             4825..5250
                     /locus_tag="PPY92_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HDJ2625732.1"
                     /translation="MKLKSFVTATLALGLLSTVGAALPSHEASADSNNGYKELTMDGK
                     HTVPYTISVDGITALHRTYFVFPENKKVLYQEIDSKVKNELASQRGVTTEKINNAQTA
                     TYTLTLNDGNKKVVNLKKNDDAKNSIDPSTIKQIQIVVK"
     gene            complement(5634..6338)
                     /gene="budA"
                     /locus_tag="PPY92_001740"
     CDS             complement(5634..6338)
                     /gene="budA"
                     /locus_tag="PPY92_001740"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDJ2625733.1"
                     /translation="MTNVLYQHGTLGTLMAGLLKGTASINELLQHGDLGIATLTGSNG
                     EVIFLDGKAYHANEHKEFVELKGDELTPYATVTKFVADTSYETKDKSSEAVFAEIKEK
                     MLSENLFSAVKISGLFKKMHVRMMPAQEPPYTRLIDSARRQPEQTETYVKGSVVGFFT
                     PELFHGIGSAGFHVHFANDDRNFGGHVLDFEVEDVKVEIQNIETFEQHFPIQDKDFTK
                     ANIDYKDIADEIREAE"
     gene            complement(6375..8039)
                     /gene="alsS"
                     /locus_tag="PPY92_001741"
     CDS             complement(6375..8039)
                     /gene="alsS"
                     /locus_tag="PPY92_001741"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HDJ2625734.1"
                     /translation="MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGP
                     ELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGG
                     QVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFI
                     SIPQDVISSPVESKAISLCQKPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSAEE
                     TNAIRKLVERTNLPVVETFQGAGVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTI
                     GYDPIEYEASNWNKELETQIINIDEVQAEITNYMQPKKELIGNIAKTIEMISEKVDEP
                     FINQQHLDELEQLRTHIDEETGIKATHEEGILHPVEIIESMQKVLTDDTTVTVDVGSH
                     YIWMARNFRSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQ
                     DLETAVRKNLNIIQLIWNDGKYNMVEFQEEMKYKRSSGVDFGPVDFVKYAESFGAKGL
                     RVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN"
     gene            complement(8533..8715)
                     /locus_tag="PPY92_001742"
     CDS             complement(8533..8715)
                     /locus_tag="PPY92_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625735.1"
                     /translation="MKDSIFWKKAFIPVYFIVAMLVFLLFRFYIKTDNFSIYLMIIFL
                     ICLGTAFIIYNYKNNR"
BASE COUNT         3177 a         1572 c         1381 g         3112 t
ORIGIN      
        1 ggaaaatttg ggtgatcgat cgtttatatg catcaacatt ctttgtggat tgtttgacag
       61 tagacagtca tttgtatacg ttaacgaatt ttgaaaattt gaatcattat ttaatcatat
      121 tattaattgc gcacgcacaa cataaaaagg agacatgtca atgacatata gaatgaagaa
      181 atggcaaaaa ttatccacca ttacgttatt aatggctggt gtgattactt tgaatggtgg
      241 tgaattcaga agtattgata aacatcaaat cgctgtggct gatacgaatg ttcaaacgcc
      301 agattatgaa aagttgagga acacatggtt agatgttaac tacggttatg ataagtatga
      361 tgagaagaat gacgcaatga agaagaagtt tgatgctacg gagaaagagg cagagaaatt
      421 actcagtagc atgaaaactg aaagtggaag gacttacttg tgggatagtg caaaagattt
      481 agataacaag tctgcggata tgactcgtac ctatcgtaat attgagaaaa tcgcagaagc
      541 gatgaggcat cctaaaacca ctttaaatac tgacgaaaat aagaagaaag tgaaagatgc
      601 ccttgagtgg ctgcataaaa atgcatatgg aaaagaacca gataataaag taaaagaatt
      661 gactgagaat tttaaaataa ctgattcatc aaagaaaaaa gctttaaatt ggtgggatta
      721 tgaaattgga acacctagat cattaacaaa tacgcttata ttgctgaatg atcaattttc
      781 aaatgaagaa aagaaaaaat tcactgcccc tattaaaact ttcgccccaa aaagtgatga
      841 aatattatct tctgtaggaa aagctgaacc tgctaaaggc ggaaatttag tagacattgc
      901 taaagtaaaa cttttagaaa gtattatcga agaagataaa gatatgatga aaaattcaat
      961 agattcattt aataaagtct tcacttacgt tcaagattct gccactgata aagaacgtaa
     1021 tggattttat aaagatggtt catatatcga ccataaagat gtcccataca ctggtgctta
     1081 tggcgttgta ctcttagagg ggatttctca aatgatgccg atgataaaag aaacaccttt
     1141 taatgataaa acccaaaata atacaacctt aaaatcatgg attgacgacg gatttttacc
     1201 actcatttat aaaggtgaaa tgatggattt atcacgtggt agagccatta gccgtgaaaa
     1261 tgaaacgagt cactcagcat ctgcaactgt aatgaaatca ttgttgagat taagtgatgc
     1321 catggatgat tcaacaaaag ctaagtataa aaaaattgtc aaaacttcag ttaaatctga
     1381 ttcaagttac ggccaaaatg atacattaag ctcttattcg gatataagca aaatgaagtc
     1441 tttaatggaa gacagcacta tttctactaa cggtttaaca caacaactta aaatatataa
     1501 tgacatggat cgtgtcacct atcacaataa agacttagac tttgcatttg gtttaagtat
     1561 gacgtcgaaa aacgtcgcac gttacgaaag tatcaacgga gagaacttaa aaggttggca
     1621 cactggtgct ggaatgtctt atttatacaa tagcgatgtg aaacactacc gtgataactt
     1681 ctgggcgaca gctgatatga aacgtttagc aggtacaaca actttagaaa atgaagaacc
     1741 taaaggtacg gatgttaaaa agtcgagtaa aacttttgta ggcggaacaa aattcgatga
     1801 ccaacatgct agtatcggaa tggattttga aaatcaggac aaaactttaa ctgccaaaaa
     1861 atcatatttc atattaaacg ataaaattgt cttcttagga actggcatta aaagtactga
     1921 ttcatcaaag aatccagtta caacgattga aaatcgcaaa gcgaatgggt atacgttata
     1981 tacagacgat aaacaaacaa ccgcttccga taatcaggga accaattccg tctttttaga
     2041 gtcaaccaat aaacctaaaa acaatatagg ctatcatttt ttaaacgaat cgaaaataac
     2101 tgtaaaaaaa gaaagtcata ctggtaagtg gagtgatata aataaaagtc aaaagcaaga
     2161 cagtaaaact aatcaatatt atgaagtaac tcaaaagcat tctaatacag atagtaaata
     2221 tgcatatgtg ttgtatccag gcttatctaa agatgacttt aatacaaaaa aagataaagt
     2281 aactgtcgtt aaacaagatg atgacttcca cgttgtgaaa gataatgaat cggtttgggc
     2341 tggtgttaat tatagtaata gcactcaaac ttttgacatt aacaacacta aagtcgaagt
     2401 taaagccaaa ggaatgttta ttcttaaaaa caaagatgat aacacttatg aatgtagctt
     2461 ctataatccc gaatctacaa ataccgcttc agatattgaa tctaaaattt caataactgg
     2521 atactctatt acaaacaaaa atacatcaac ttctaatgaa tccggcgtac actttgaatt
     2581 aactaaataa gcaagaaaat ctcgacacta taccattttt atagtgtcga gattttataa
     2641 gtatcgccat cattcacctt gtaatccgca gaccacatcc cctttgataa gttctagatt
     2701 atttaaaaat cgaattttca aagacgtaca tgcttcaata ttcttatcat tcattacttg
     2761 gaaatgtata tgaggttcag tagaattgcc agaattccca accttcccaa ggaagtcacc
     2821 atatttaaca ttttgcccct cattcacaat gattgaatat tgttgtaaat gagctattaa
     2881 gctatactca ttttctgcgt gcttaattac aatataattt cctaaaaact gtgattcatt
     2941 cgtacttcca atactgttgt cttgaacccc attcactact ttttcaactg taccatttgc
     3001 tggcgcaatg atatcttttc caaaacaaaa ataatcagta tttacttttc cttgattctt
     3061 atatgtttta tgattttctt taataactaa atcaatagca tacctttgat gtaggtataa
     3121 atagtgataa ttatataatt cattatctcc accccaaaaa acctcatagc aatctgttgt
     3181 cggcactgcg tattttaaag tcgtttcatt cttaatcgca tcataattga ttaacggacg
     3241 cataacaaat ccgataatac tattatcttt ccaagtcact gccactccaa attgatttgc
     3301 tttatctatc catatttgct ccatagtatc atcattttga gtacacactt ttaactcatg
     3361 tacatcagac tgccttgtat aagatttgaa aagtttccta aactgccaat aactaaactc
     3421 tcgcttcaaa ttttgatcaa aaatcttata tattttcctg ctcttacttt tatttaaata
     3481 atccccaaag tttgttttat tcatatatac ttaacctctt atattaattt tggtatcaat
     3541 tctttctcca gttcctgcgt catttttgcg ctacctgaat cgtctacact taattggaca
     3601 aatcaacata taaataattt tttaaaacta aagtgtagtc tattcaccat attttctaat
     3661 catcccttaa atattaatat ttgcacttaa aaagtataca tatcactcaa ctaatcaata
     3721 atcatactta ccttcaacaa attctttaac atgcagtgtt atcccttgat aatatggata
     3781 ttttgttaat ctaaatttct gctttaatgc ttcatctcta ctattcgaca tcaaatatgc
     3841 atgttcaaga tagcttaaat atgcttcgtc attgccgcta tctccaaaac ctaatattga
     3901 ttttactggt atattgtatt ttttcatcag aaattgagta gcatataatt ttccagcatt
     3961 tgacggtgtg aagtcaatat catacgcatt ttcaggatca ccagctaatg gattgcttcg
     4021 actaaatctc gctgtatagt tttggtcatt tgcgtatctg acaagtgctt ctaggatacg
     4081 actatcattg ttgtgattac ctgttgaatg aaaataaaaa ttataatgtg tttcattacc
     4141 ttcaaatgct ctttgtggaa taaattcgac attatattgc gcagaaatat gacgtaatat
     4201 atcttcaata tcttcaaata aaaatggttc gctcaagact ttctttttat atgtttctga
     4261 ttcaacatat tcgccattat tccaagtgaa cattttagaa ctcaaatcag aaaagatatg
     4321 taatggttta aacgtcatat taacttgatc cattttatcc atgacacttt gaaatgtact
     4381 tcctgtcaat acagcagtga tcacttcatt attattagtt agtttttcca acaatttttc
     4441 catttctctt aaatgactta aatcttcttc atttgtatta tgtttaaaat atgtttcatc
     4501 aaaatcaaac aataatagac gctttgacat actcattccc ccataatatt ctaatgaatt
     4561 tatctataac ttaaatacac tataagtctc aattaaaagt ataagatcga ctatatgctt
     4621 ttcatgctta ttatatacaa ctaatgaatc cctataaact aaaaacattg cttaccactt
     4681 ttcaaaatac acgggtcata tccctcgtta aaaactatta ctaaaaatca aaaatataaa
     4741 aaaatcaaaa agtatgtttt aaactttatg aaacgtgtta tataaatagt gtaatatacc
     4801 acaactttaa aaggagattg agatatgaaa ctaaaatcat ttgttactgc cactttagca
     4861 ttgggattat tatcaacagt aggtgcagct ttaccgagtc atgaagcatc tgcagatagt
     4921 aataacggtt ataaggaatt gacaatggat ggaaaacaca ctgtgcctta cacgatctca
     4981 gtagatggta ttactgcatt acatcgaact tactttgttt ttccagaaaa taaaaaggta
     5041 ctttatcaag aaattgacag taaagtaaaa aatgaattag cttcacaacg tggtgttaca
     5101 acagaaaaaa ttaataatgc acaaacagca acgtatacac ttactttgaa tgatggtaat
     5161 aaaaaagtag tgaatctaaa gaaaaatgac gacgctaaaa attcaattga tccaagtaca
     5221 atcaaacaaa tacaaattgt agttaaataa taatttgaaa atgaaaatag ttacacaggg
     5281 caaacgctga aaaattgcgt ttgtcttttt agtatgaaat gaatgatcga acttataatt
     5341 tcttattgat tgcttctact gacaatagat tagatcggcc tatactaaag taaattctat
     5401 attaatgtct tccgcaattt ataatctaac ataacagcaa atctcaaata tctggaacct
     5461 aataaatccc ctcactactg ctaatagtga agggatttat tttattactt agacgtaatt
     5521 ataaatatat ttgttctatt gtagtcatgg ttaatcatat ttacaaacta tcatttctaa
     5581 atcgcgcatt atgaagtcaa ttttcataaa ccgtcattat atttctggat tcattattca
     5641 gcttctctaa tttcgtctgc aatatcttta tagtcaatat ttgctttagt gaaatcttta
     5701 tcttgaattg gaaaatgctg ttcaaatgtt tctatatttt ggatttctac tttaacatct
     5761 tctacttcaa aatctaagac atgtccacca aagttacgat catcattcgc aaagtgtaca
     5821 tgaaatcctg ctgatccgat accatggaat aattctggtg taaagaaacc aacgactgaa
     5881 cctttgacat acgtttcagt ttgttcaggt tgtcttctag ctgaatcgat taaacgtgta
     5941 taaggtggtt cttgagccgg catcatacgt acatgcattt ttttaaataa gcctgaaatt
     6001 tttactgctg aaaataaatt ttcactcaac atcttttcct taatttctgc aaaaactgct
     6061 tctgaagatt tatctttcgt ttcatagctt gtatctgcta caaatttagt tacagttgca
     6121 tatggtgtta actcgtcacc ttttaattct acaaattctt tatgttcatt tgcatggtaa
     6181 gcttttccat ctaaaaagat tacctcaccg tttgaacctg ttagtgtagc gatacctaag
     6241 tcaccatgtt gcaataattc atttattgat gcagttcctt ttaataatcc tgccattaat
     6301 gtgcctaatg taccatgttg gtataaaaca ttagtcataa aatctcccac tcctctttaa
     6361 catttatctt tattttagtt aaatacgtca ggtaacatat ttgttgaaag tttgatatta
     6421 tctttgtaat ttacaggtat atcaattaat actggaccat ctgtttcata gccctcttta
     6481 attgccgctt ctaattcttc ttgattagta actcgtaaac ctttcgcgcc aaatgattct
     6541 gcatatttta caaaatctac aggaccgaag tctacacctg acgaacgttt atatttcatt
     6601 tcttcttgga attcaaccat gttatatttt ccatcattcc aaataagctg gatgatattt
     6661 aaatttttac gtacggccgt ttctaaatct tgtgatgaaa ataaaaagcc accatcgcca
     6721 gcaacggaca caacttgcgt attagggcgc acaagtgcag ctgaaattgc ccacggtaat
     6781 gctacaccaa gcgtttgcat accattgcta aataataaat gtcttggatt gtaacttctg
     6841 aaattacgtg ccatccaaat atagtgactt ccaacatcaa ctgttacagt agtatcatca
     6901 gttaatacct tttgcataga ttcaataatt tccactggat gtagaattcc ttcttcatgc
     6961 gtcgctttaa taccagtttc ttcatcaata tgtgttctta attgttctaa ttcgtctaaa
     7021 tgttgttgat ttataaatgg ctcatccact ttttcagaaa tcatttcaat cgttttagca
     7081 atattaccaa tcaactcttt tttcggttgc atataattag taatttcagc ttgaacttcg
     7141 tcaatattga taatttgtgt ttctaattct ttattccagt tactagcttc gtattcaatt
     7201 ggatcataac cgattgtaac aactaaatca cttttacgta ataattcatc accaacttga
     7261 ttgcggaata agcccacacg accgaagaaa tgattttcta attcacgact aattacacct
     7321 gcaccttgga atgtttctac aactggtaaa ttcgtgcgct caactaattt gcgaatggca
     7381 tttgtttctt ctgcacttga acttctcata ccagctaata aaacaggaaa tgatgcattt
     7441 ttaatcgctt caatgacatc attaatatct tgttcactcg gtactcctaa atttggtttt
     7501 tggcaaagtg atatagcttt agattcaact ggtgaagaaa taacgtcttg cggaatacta
     7561 ataaaacttg cgccattttt tcctgaagta gcaattcgaa ttgcatttgt cataacttct
     7621 gataatgatt caggatcttg tacttcttca ctgtattttg atgaatattt taatagcgca
     7681 gcattatcaa tactttgatg cgttaatcgt aataaatcat tacgtttcac ttggccacct
     7741 aacgctaata caggatcccc ttcagatgta gctgttaata gtccagtcgt taaattactt
     7801 acaccagggc cacttgtaac aagtactaca cccggtttac ctgttaatct tccaatacct
     7861 tgtgccatca ttgcagcatt ttgttcatga cgagtgacaa taagttcagg accatcatca
     7921 attaaagcat taaatagata gtctatcttt gcacccggaa taccaaaaac atattctacc
     7981 ccattatttt tcaaagtatc aataaccata tcggctgcag tgtacttttt atcagtcatt
     8041 tatattcatt tccttccaat tgtgatgtat agatgtgtat tacatgtaat tacattgtga
     8101 tttgtaatct aatgatagtt acgcaaaatt aatgatgttt atcaaaattt tacatgtacg
     8161 attagatttg taatacataa aaattgcctt cattacatta tataaccttt gttagatgaa
     8221 taaaaatcgt tatttgtaat actaagtatt ccaaaaatgc atgagtagat tttgtgcgtt
     8281 gtgattatga gggatgttgg attgattgat gtataggatt gttacgatat tttgggatga
     8341 ggtgtttagt caatggcggt ttgaaattgt cgttttatgg tgggctgaaa atgctgtttt
     8401 atgtcgggct tgaatttgtc gttttatgga gatataaaaa tgctgtttta tggcggtttc
     8461 aaaatggtgt ttcgattaat aagccacagc aagacttaga tctaactatg acttatacat
     8521 ttcacactta tattagcgat tatttttata gttataaatg ataaatgcag ttcctaaaca
     8581 aattaagaag ataatcatta agtatataga aaagttatct gttttaatat aaaacctaaa
     8641 aagtagaaac accagcatcg caacaataaa ataaacagga ataaaagctt tcttccaaaa
     8701 tatagaatct ttcaattagg tcactcctta ttttgataat actctttaat taaggctctt
     8761 gagttatcgt ccggtcgttc tttcccttga accactatga ttagaacaca atcgtctggt
     8821 tatcgtccac cctccgcaag ctaaatgaca atttaataat gtacactttt gattgtgtaa
     8881 acatgtacaa attatacgtg taaatcaata atccatagtt gaattaattc tattcctaag
     8941 acattaaata aaggaaagtc catttatact ctacacattt catattcccc acacaaacgc
     9001 aaaaaaacac ctcgacataa catcgaggtg ttttgtatat atcgtccgac aattaacgtt
     9061 ttaagaaatg aggtgaacga cgagctgctt taagacctgg tttactttag gatttcgtat
     9121 agttgcatat actgtcttaa ggcatgatat taaaggattt ctaatttttt tgatagtatt
     9181 gggtttcctc taatttaacc taggggtatt aaaatataat aaaaatctgc tggctggtgg
     9241 ta
//
LOCUS       DANBWH010000109         9119 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.112, whole genome shotgun sequence.
ACCESSION   DANBWH010000109 DANBWH010000000
VERSION     DANBWH010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9119
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.112"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(276..1541)
                     /gene="lysA"
                     /locus_tag="PPY92_001743"
     CDS             complement(276..1541)
                     /gene="lysA"
                     /locus_tag="PPY92_001743"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDJ2625736.1"
                     /translation="MTVKYNQNGELTMDGISLKTIAQSFGTPTIVYDELQIREQMRRY
                     HRAFKDSGLKYNISYASKAFTCIQMVKLVAEEDLQLDVVSEGELYTALEAGFEPSRIH
                     FHGNNKTKHEIRYALENNIGYFVIDSLEEIELIDRYANDTVQVVLRVNPGVEAHTHEF
                     IQTGQEDSKFGLSIQYGLAKKAIDKVQQSKHLKLKGVHCHIGSQIEGTEAFIETAKIV
                     LRWLKEQGIQVELLNLGGGFGIKYVEGDESFPIESGIKDITDAIKSEIKVLGIDAPEI
                     GIEPGRSIVGEAGVTLYEVGTIKEIPEINKYVSIDGGMSDHIRTALYDAKYQALLVNR
                     NEEADDSVTIAGKLCESGDIIIKDAKLPSSVKRGDYLAILSTGAYHYSMASNYNQMQK
                     PSVFFLKDGKAREVIKRQSLRQLIINDTK"
     gene            complement(1531..2616)
                     /locus_tag="PPY92_001744"
     CDS             complement(1531..2616)
                     /locus_tag="PPY92_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDJ2625737.1"
                     /translation="MTATWSVNKKIFLQNAITVKNNQPLMAVVKNNAYHYDLEFAVTQ
                     FIHAGIDTFSTTSLREAIQIRQLAPDATIFLMNAVYEFDLVREHQIHMTLPSLTYYYN
                     HKNDLAGIHVHLEFENLLHRSGFKDLNEIKEVLKDHHHNQNAKMIISGLWTHFGYADE
                     FDVSDYNVERSQWMEIVEALLSEGYQFDLIHAQNSASFYREGQILLPHHTHARVGIAL
                     YGSRPYSSLNQHDIVQSLTVKAHVIQVREVQAGDYCGYSFAFEVTKNNTKLAVVDIGY
                     GDGILRTRAKHEALINGKRYPIRALMMSHMFVEVDGNVHAQDEVILYNNDIRIDEYTF
                     KGVGANSEQLSAMNHDSLKKEYISNDC"
     gene            complement(2621..3772)
                     /locus_tag="PPY92_001745"
     CDS             complement(2621..3772)
                     /locus_tag="PPY92_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDJ2625738.1"
                     /translation="MNELEFVTKHRRHLHQHPELSLHEFETTAYIKAFLDSLNIKYDC
                     PLETGVIAYLEGNGSHTIAYRADIDALPILEENDVPYRSQSDHVMHACGHDGHTTALM
                     LFVQRCKDMQDAGQLPQNVVFIFQPAEETGGGANRLIKAGAFDKYPIEAVFGIHVNPF
                     ADEGIAVIRDEEITASATEYRFFLTGLSSHVADKEQAHSCGEALQHVLTQISQIQQFH
                     LNGLKRNIVHIGHFKAGEAINTVPSNGYLEGTIRTYDIDDLTIVKNQMHKIAESVKLL
                     FNVECEVKFAEGYPPTINSPKLRTQIEDALIKADLNVYDKPTPFLFGEDFSFYGQQLA
                     PAYFVFIGTRNEDKGFVTGLHTSHLNFDEKALINVVNFYENLLNNYKEV"
     gene            complement(3915..4634)
                     /gene="dapD"
                     /locus_tag="PPY92_001746"
     CDS             complement(3915..4634)
                     /gene="dapD"
                     /locus_tag="PPY92_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDJ2625739.1"
                     /translation="MVQHLTAEEIIQYISDAKKSTPIKVYLNGNFEGITYPESFKVFG
                     SEQSKVIFCEADDWKPFYEAYGSQFEDIEIEMDRRNSAIPLKDLTNTNARIEPGAFIR
                     EQAIIEDGAVVMMGATINIGAVVGEGTMIDMNATLGGRATTGKNVHVGAGAVLAGVIE
                     PPSASPVIIEDDVLIGANAVILEGVRVGKGAIVAAGAIVTQDVPAGAVVAGTPAKVIK
                     QASEVQDTKKEIVAALRKLND"
     gene            complement(4661..5383)
                     /locus_tag="PPY92_001747"
     CDS             complement(4661..5383)
                     /locus_tag="PPY92_001747"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDJ2625740.1"
                     /translation="MKILLIGYGAMNQRVARLAEEKGHEIVGVIENTPKATTPYQQYQ
                     HIADVKDADVAIDFSNPNLLFPLLDEEFHLPLVVATTGEKEKLLNKLDELSQNMPVFF
                     SANMSYGVHALTKILAAAVPLLDDFDIELTEAHHNKKVDAPSGTLEKLYDVIVSLKEN
                     VTPVYDRHELNEKRQPQDIGIHSIRGGTIVGEHEVLFAGTDETIQITHRAQSKDIFAN
                     GAIQAAERLVNKPNGFYTFDNL"
     gene            complement(5380..6267)
                     /locus_tag="PPY92_001748"
     CDS             complement(5380..6267)
                     /locus_tag="PPY92_001748"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDJ2625741.1"
                     /translation="MTHLFEGVGVALTTPFTNNKVNLEALKAHVNFLLENNAQAIIVN
                     GTTAESPTLTTDEKERILKTVIDLVDKRVPVIAGTGTNDTEKSIQASFQAKALGADAI
                     MLITPYYNKTNQRGLVKHFEAITDAVKLPVVLYNVPSRTNMTIEPETVEILSQHPYLV
                     ALKDATNDFEYLEEVKKRIDTNSFALYSGNDDNVVEYYQRGGQGVISVIANVIPKEFQ
                     ALYDAQQSGLDIQDQFKPIGTLLSALSVDINPIPIKALTSYLEFGNYELRLPLVSLED
                     TDTKVLREAYDTFKAGENE"
     gene            complement(6269..7258)
                     /locus_tag="PPY92_001749"
     CDS             complement(6269..7258)
                     /locus_tag="PPY92_001749"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDJ2625742.1"
                     /translation="MTKLAVVGATGLVGTKMLETLNRKNIPFDELVLFSSARSAGQEV
                     EFQEKTYTVQELTDARASEHFDYVLMSAGGGTSEHFAPLFEKAGAIVIDNSSQWRMAE
                     DIDLIVPEVNEPTFTRGIIANPNCSTIQSVVPLKVLQDAYGLKRVAYTTYQAVSGSGM
                     KGKKDLAEGVNGKAPEAYPHPIYNNVLPHIDVFLENGYTKEEQKMIDETRKILNAPDL
                     KVTATCARVPVQDSHSVEIDVTLDKETTAEDIKALFDQDDRVVLVDNPENNEYPMAIN
                     STNKDEVFVGRIRRDDSLENTFHVWCTSDNLLKGAALNAVQVLEQVMRLKGAN"
     gene            complement(7322..8527)
                     /locus_tag="PPY92_001750"
     CDS             complement(7322..8527)
                     /locus_tag="PPY92_001750"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDJ2625743.1"
                     /translation="MVTRSVLKFGGSSVSDFTKIKRIAEMLKERVNQDEQLIVVVSAM
                     GNTTDQLMTNVSTLTKAPKQQELALLLTTGEQQTVSYLSMVLNDIGVNAKAMTGYQAG
                     IKTIGHHLKSKIAQINPQTFEQAFQENDILVVAGFQGINEHQELTTLGRGGSDTTAVA
                     LAVSNQIPCEIYTDVDGVYATDPRLLPKAKRLDIVSYEEMMEMSALGAGVLETRSVEL
                     AKNYNIPLYLGKTLSNVKGTWIMSNEEILEKKAVTGVALDKHMMHVTISYPLPDNQLL
                     TQLFTELEEGAVNVDMISQIVNLDGLQLSFTIKDSDFHQISMILETLKNQYEALAYKI
                     NEHYVKISLIGSGMRDMSGVASKAFLTLIENNIPFYQTTTSEISISYVIDDFNGQQAV
                     EKLYDAFNI"
BASE COUNT         2772 a         1712 c         1427 g         3208 t
ORIGIN      
        1 tattatttga aaaagcaagt aaagcataaa atctaatttc atgatgcttg tttaaagtgt
       61 ggtatgtata tgatgcttta aattagttga atagggctaa ctaaatataa aggattaatg
      121 acaaaaagaa ataaatactt tctaactaaa aatgtgctgt tcttcctttt ttagagtata
      181 tgtccaagca tgttttctac agtcatctat aatgcataaa aattccccga gtcacagtat
      241 aattttctgt gcttcgggga ttactttgtt tatttttatt ttgtatcatt aataatgagt
      301 tgtcttaacg attgtcgctt tataacttca cgtgctttgc catcttttaa gaaaaacaca
      361 gaaggctttt gcatttgatt gtaattggat gccatagagt aatgatatgc accagttgat
      421 aatatagcaa gatagtctcc acgtttgact gatgaaggta atttagcgtc tttaataatg
      481 atatcaccag actcacataa ttttccagct atagttacac tgtcatctgc ttcttcattt
      541 ctattaacaa gcaatgcttg atactttgcg tcataaagtg cagttctgat atgatcactc
      601 ataccgccat cgattgaaac atatttatta atctctggaa tttctttaat ggttccaact
      661 tcatataaag taacgccagc ttcacctaca attgatcgtc ccggttcaat acctatttct
      721 ggtgcatcta tacctagaac tttaatttcg gattttattg cgtctgtaat atctttaata
      781 ccactttcga tagggaaact ttcgtcacct tcaacatatt taataccaaa gccaccacca
      841 aggtttaata attcaacttg aatgccttgc tctttaagcc aacgtaaaac aattttagca
      901 gtttcaataa atgcttctgt accttcaatc tgtgaaccaa tatgacaatg tacacctttt
      961 aattttaagt gtttagattg ttggactttg tcaattgctt ttttagctaa gccatattga
     1021 attgataatc caaacttact atcttcttgc ccagtttgaa taaattcgtg tgtatgtgct
     1081 tcaacacctg gattaactcg taatacaact tgaaccgtat cattagcata gcggtctatt
     1141 aattcaattt cttctaatga atctataaca aaataaccga tattattttc taaagcatac
     1201 ctaatttcat gtttcgtttt attgttacca tggaaatgga tgcgactcgg ttcaaaacct
     1261 gcttctaaag ctgtatataa ttcaccttca gaaacaacat ctaactgtaa atcttcctca
     1321 gctacaagtt tgaccatttg aatgcaagta aatgcctttg aggcgtatga aatattgtat
     1381 tttaatccac tatctttaaa tgcgcgatgg taacggcgca tctgttctct aatttgtagt
     1441 tcatcataaa caatggtagg tgtaccaaag ctttgtgcaa tcgtttttaa actaatacca
     1501 tccattgtta attcgccatt ttgattatat ttaacagtca tttgaaatgt actccttttt
     1561 taaagaatca tgattcatag cacttaattg ttcagaattt gcaccaacac ctttaaaggt
     1621 atattcatcg atgcggatat cattattata aagaataact tcatcttgtg catgtacatt
     1681 gccatctact tcaacaaaca tatggctcat cattaatgca cgtatcgggt agcgtttacc
     1741 attgataagt gcttcatgtt tagcacgagt tcttaaaatt ccatcgccat aaccgatatc
     1801 aactacagct aattttgtat tgtttttagt cacttcaaag gcaaagctat aaccgcaata
     1861 atcaccagct tgtacttcgc gcacttgaat aacatgtgct tttacagtta atgactgaac
     1921 tatatcatgt tgattcagtg aactatatgg tcttgaaccg tataacgcaa tacctacacg
     1981 cgcatgtgta tggtggggta gtaatatttg tccttcccga taaaaactcg cactattttg
     2041 agcatggatt aggtcgaact gataaccttc agataaaagt gcttcaacaa tttccatcca
     2101 ttgtgaacgt tcaacattat aatctgacac atcgaattca tcagcatatc caaaatgggt
     2161 ccataaacca ctaataatca tttttgcatt ttgattatga tggtgatctt tcaatacttc
     2221 tttaatttcg tttaaatctt taaatccaga ccgatgtaat aaattttcaa attctaagtg
     2281 aacatgaata cctgctaaat catttttatg gttatagtaa tatgtcaacg acggcaaagt
     2341 catgtgtatt tgatgttcgc ggactaaatc aaactcgtaa actgcattca ttaaaaagat
     2401 tgttgcatct ggagcaagtt gtctaatttg aattgcttct cgtagtgatg ttgtgctaaa
     2461 tgtatctata cctgcatgga taaactgagt tacagcaaat tctaggtcat agtgatatgc
     2521 attattttta acaactgcca ttaatggctg attgtttttg actgtgattg cattttgtaa
     2581 aaatattttc ttatttacag accatgttgc tgtcaatgta ttacacctct ttgtaattat
     2641 ttaataaatt ttcgtaaaaa ttaaccacgt ttattaacgc tttttcatca aaatttaaat
     2701 gtgatgtgtg caaaccagtt acaaaacctt tatcttcatt tcgtgttcct ataaaaacaa
     2761 agtaagctgg agctagttgt tgaccataaa aactaaaatc ttccccaaat aagaatggcg
     2821 ttggtttgtc atagacattt aaatcagctt ttattaaggc gtcctctatt tgagtacgta
     2881 atttcggact attgattgta gggggataac cttctgcaaa tttaacttca cattctacat
     2941 taaacagaag cttgacactt tctgctatct tgtgcatttg atttttaacg attgttaaat
     3001 catcaatatc atatgtacga attgtacctt ctaaataacc attacttggt acagtgttaa
     3061 tcgcttcacc agctttaaaa tgaccaatat gaacaatatt tcgtttcaaa ccgttaaggt
     3121 gaaattgttg aatttgtgat atttgagtta atacatgttg taatgcttca ccacaagaat
     3181 gagcttgttc tttatcagca acatgacttg acaggcctgt taagaaaaag cgatactctg
     3241 ttgcgctggc cgtaatttct tcatctctta tcactgcaat gccttcatca gcaaatgggt
     3301 taacatgaat accaaatacc gcttcaattg gatacttatc aaaggcaccg gcttttatta
     3361 atcgatttgc accgccacca gtttcttctg caggttggaa aatgaaaacg acattttgcg
     3421 gtaattgacc tgcgtcttgc atgtctttgc aacgttgtac aaaaagcatt aatgcagttg
     3481 tatgaccatc atgtccacaa gcatgcatca catgatcaga ttgactgcga taaggcacat
     3541 cattttcctc taaaataggt aacgcatcaa tatcagctct atacgctatc gtatgtgagc
     3601 cattaccttc taagtatgca atgacgccag tttccaatgg gcaatcgtat ttaatattta
     3661 aactatctaa aaacgcttta atataagcag ttgtttcaaa ttcatgtaag cttaattcag
     3721 gatgttgatg taaatgacgg cgatgtttcg taacaaattc taattcattc ataattatca
     3781 atcctttgtg ttaaattact atataaatag tgtaacgtat ttcgaaattt gtgatcataa
     3841 gtttattcaa tgataaacaa taaggttgag acataatcgt atctcaacct tgaaattatt
     3901 atacgttgac gtcactagtc attcagtttt cttaatgctg ctacaatctc ttttttagta
     3961 tcttgtactt cagaagcttg cttaatcact tttgcaggtg taccagcaac aactgcacca
     4021 gctggtacat cttgtgtcac aatcgcgcca gctgcaacaa tagcaccttt accaacacgt
     4081 acaccttcta aaataactgc atttgcaccg attaatacat catcctcgat tataactggt
     4141 gatgcactag ggggttcaat cacacctgcc aatactgcgc cagcccctac atgtacattt
     4201 ttaccagttg tagcacgacc accgagagta gcattcatat caatcattgt accttcgcca
     4261 acgactgcgc caatattaat tgttgcgccc atcataacga cagcaccatc ttcaataatg
     4321 gcttgttctc taataaatgc acctggttca atacgtgcat tcgtatttgt taagtctttt
     4381 aatggaatgg cagaattgcg acgatccatt tcaatttcta tatcttcgaa ttgactacca
     4441 tatgcttcgt aaaaaggttt ccaatcatcc gcttcacaaa agattacttt agattgttct
     4501 gaaccaaata ctttaaaact ttctgggtat gtgatgcctt caaaattacc atttaaatat
     4561 acttttattg gtgtagactt tttagcatca cttatatatt gaataatttc ttcagctgtt
     4621 aaatgttgta ccataaaata atcgatctcc tttaatatgt ttataagttg tcaaacgtat
     4681 aaaagccgtt tggtttatta actaagcgtt ctgctgcttg tatagcacca ttcgcaaaaa
     4741 tatcttttga ttgtgcacga tgtgtgattt gaatcgtttc atcagtgcca gcaaatagaa
     4801 cttcatgttc accgacaatc gtacctccac gaatagaatg tataccaata tcttgtggct
     4861 ggcgtttttc atttaattca tgtctatcat acacaggtgt tacattttct ttcaaagata
     4921 cgatcacatc atacaatttt tctaacgtac cacttggtgc atctactttt ttattatgat
     4981 gtgcctcagt caattcgatg tcgaaatcat caagtagggg aacagcagct gctaaaattt
     5041 tagtcagtgc atgaacgcca taactcatgt tcgcgctgaa aaatacaggc atattttgac
     5101 tcaattcatc taacttatta agtagttttt ctttctcgcc agttgttgcc acaactaatg
     5161 gcaaatgaaa ctcttcatct aataatggga ataatagatt cggatttgaa aaatctattg
     5221 caacatcggc atctttaaca tctgcaatat gttgatattg ttgatatggc gttgttgctt
     5281 tcggtgtatt ttcaatgacc ccaacgattt catgtccttt ttcttctgct aatctagcaa
     5341 cgcgttgatt cattgcacca tagccaatta gtaatatttt cactcatttt cacccgcttt
     5401 aaatgtgtca tatgcttcac gaagcacttt agtatctgta tcttctaggc taaccaatgg
     5461 taaacgtaat tcatagtttc caaattctaa ataacttgtt aaagctttaa taggaattgg
     5521 gttaatatca actgataggg ctgataacag tgtgccgatt ggtttaaatt gatcttgaat
     5581 atctaatcca ctttgttgag catcgtataa cgcttgaaat tctttaggaa tgacattggc
     5641 aataacagag ataacccctt gaccgccacg ttgatagtat tcgacgacgt tgtcatcatt
     5701 gccactatat aatgcaaatg aatttgtatc aattcgcttt ttcacttctt ctaaatactc
     5761 aaaatcattc gtagcatctt ttaaagcaac taaataagga tgttgactta atatttctac
     5821 agtttctggt tcaattgtca tgttcgttct tgaaggaaca ttgtacagca cgactggtaa
     5881 tttcacagca tctgtaatcg cttcaaagtg tttgactaaa ccacgttggt tcgttttgtt
     5941 gtagtagggc gtaattaaca taattgcatc agcccctaag gctttagctt ggaatgaagc
     6001 ttggattgac ttttcagtat cattagttcc agttccagct atgacaggaa cacgtttatc
     6061 tacaagatca ataactgttt ttagaatgcg ttctttttca tctgttgtta aagtagggct
     6121 ctcagcagta gttccattaa cgatgattgc ttgggcatta ttttctagta aaaaattaac
     6181 gtgtgctttc aaagcttcaa gattaacttt gttatttgta aaaggggttg taagtgcaac
     6241 gccaacaccc tcaaataaat gtgtcatttt aattcgctcc ttttaaacgc ataacttgtt
     6301 ccaatacttg tacagcattt aatgcagcac cttttaataa attgtctgat gtacaccata
     6361 catggaaagt attttctaat gaatcatctc tacgtatacg gccaacaaac acttcatctt
     6421 tattagtgga attgattgcc attggatatt cattgttctc tggattgtct actaaaacaa
     6481 cgcggtcatc ttgatcaaat aacgctttaa tatcttctgc tgtcgtttct ttgtcaagcg
     6541 ttacatcaat ttcaacacta tgactatctt gaacaggcac acgtgcgcat gttgctgtta
     6601 cttttaagtc tggcgcattt aaaatttttc tcgtctcatc aatcattttt tgttcttctt
     6661 ttgtatatcc gttttctaaa aacacatcaa tatgcggtaa cacattatta taaattggat
     6721 gtggatatgc ttctggtgct ttaccattta caccttcagc taaatctttc ttacctttca
     6781 tccctgaacc tgatacagct tgatatgttg tatatgccac tcgttttaaa ccataagcat
     6841 cttgcaatac ttttaatggc acaacagatt gaatcgtaga gcagtttgga ttggcaatga
     6901 tacctcttgt aaatgtaggt tcattgactt ccggaacgat taaatcaata tcttctgcca
     6961 tacgccattg acttgaattg tctataacga ttgcaccagc tttttcaaag agtggggcaa
     7021 agtgttcgct tgtaccgcca ccagcactca ttaatacata atcgaaatgt tcactagcac
     7081 gagcatcagt taattcttgt actgtatatg ttttttcttg aaattcaact tcttgccctg
     7141 cagaacgtgc tgatgaaaat aatactaatt catcgaaagg aatattttta cgatttaatg
     7201 tctccaacat ttttgtgcct actaatcctg ttgcacccac aactgctaac tttgtcataa
     7261 cttgtcactc cattttataa taatttccaa tttttagaat attttaacaa tcattttacc
     7321 attaaatgtt aaatgcgtca tatagttttt ctaccgcttg ttgcccatta aaatcatcaa
     7381 tgacgtatga aatacttatt tcagatgttg ttgtttggta gaaaggtata ttattttcaa
     7441 ttaatgtcaa aaatgctttt gatgccacac ctgacatatc acgcatgcct gagccaatta
     7501 atgaaatttt gacataatgc tcattgattt tataagctaa tgcttcatat tgattcttta
     7561 atgtttcaag aatcatagaa atttgatgaa aatcactatc tttaatcgtg aaggatagtt
     7621 gtagcccatc caagttgacg atttgtgaaa tcatatcaac atttacagca ccttcttcaa
     7681 gttccgtaaa tagttgggta agtagctgat tgtcaggtag gggataacta attgttacat
     7741 gcatcatatg tttatccaaa gccacaccag taactgcttt tttctctaat atttcttcat
     7801 ttgacataat ccatgttcct ttcacgttcg ataaagtttt tcctaaatat aaagggatat
     7861 tatagttttt agctaattca acacttcttg tttcaagtac accagcacct aaagcgctca
     7921 tttccatcat ttcttcatat gagacgatgt ctagtcgttt agcctttggt aaaagtcttg
     7981 ggtcagtggc atacacacca tcaacgtcgg tataaatttc acaaggtatt tgattactaa
     8041 cagcaagtgc cacagcggtc gtatcagaac cgcctctgcc taaagttgtt aattcctgat
     8101 gttcattgat gccttgaaat ccagcaacta ctaaaatatc gttttcttga aaggcttgtt
     8161 caaatgtttg aggattaatt tgagcaattt tactttttaa atgatggcca atggttttaa
     8221 tacccgcttg atagccagtc attgctttgg cattcacacc gatatcattt aataccattg
     8281 ataaataaga tacagtttgt tgctctccgg ttgtcaataa taatgccagt tcttgttgtt
     8341 ttggtgcttt agtcaaggtc gatacattcg tcattaattg atctgttgtg ttacccatag
     8401 cacttacaac gacaattaat tgttcatctt gattgactcg ctcctttaac atttcagcga
     8461 tcctttttat ttttgtaaaa tcactgacgg atgatccgcc aaatttcaac acacttcttg
     8521 ttaccatata atcctcctaa ttgttaggaa gggcacacaa taataaaaaa agaacaagtt
     8581 cggaatgcga acttgctctt aatctatata caagtgatgc actccattat ttttaaataa
     8641 tgacaaactc tcagctctta accaaaaagt ccaacaaatt ataactgcta ttataatcgc
     8701 ttcggcatcg caccctttca aattcagctg ttagcagaca gtaatctaaa ctttactcat
     8761 gattgatgcg cctcatcaaa atattattaa attggtatgt gttcattata catatgacaa
     8821 atatgaatgt aaaccgataa tttagatttt ttggaataac ctgaaaattc aagttatagc
     8881 gttgcttata ttttaaaagg tggtgataat gagacttttt gaaaaataaa attcaaaata
     8941 cttatagcat actcaatatg cacattaaat aaatgtactc ttttaatgcg gtgataactg
     9001 tatttgtaat ttagagaagg ggtgttcact gtgctgatgt gctaaaaaat aaaataaaaa
     9061 ggacacgagg ctgggatatt aagttcttag gcaatgtaaa aagaggctga gacggcacc
//
LOCUS       DANBWH010000110         8719 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.113, whole genome shotgun sequence.
ACCESSION   DANBWH010000110 DANBWH010000000
VERSION     DANBWH010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8719
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.113"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            68..526
                     /locus_tag="PPY92_001751"
     CDS             68..526
                     /locus_tag="PPY92_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625744.1"
                     /translation="MKKLLITIVIIVLIGALGFAIYAAINHSTSSSNNQEQENKSTHK
                     KTETEENDQQDDSADKTDKNQNNGNNDTNDNQPSSPSNVPNNHSTVPSNTQPAQPNDS
                     NSNKNNTHGGGSQSSTNNQNNQQQNTPPANKSNNTNTPSKQTPQAQSPKN"
     gene            766..2640
                     /locus_tag="PPY92_001752"
     CDS             766..2640
                     /locus_tag="PPY92_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDJ2625745.1"
                     /translation="MLDRHLKLLQFFIKNPSKHISSNEIAEHVNVSNRTVRNDIHVIN
                     SNFMDDIIVSIKSKGYQLNTSQYTLETITERYTHIQSYKEKLLLSMAYQLLMHNKSQT
                     LQQLEQDYLLSKTVLNDYFVRIQQWCQKFNIVLTIKKKQGIVVDGTDNDITNAIIHLN
                     QLSSGHVHVEDLILNELPDSHQRMISHIIQETLQQHSIETTDIQIQQLLIHLILIIKR
                     KQSLSELDTLNHDAKLISQSCIKQINERLGYDLNEKVINMFSFFIAYHFNKLDIGIER
                     IFIQSYIDRLIELMQQRIHVPFNEDSILQQNLYNHFSKAYLRITQNIYLNNPLVIEIK
                     KLYPFVFNTLFEAIDKLAIDTDIEMSEDEIAFLTIHFQAAIERRTKTQLNVVIACYYG
                     LGVSNFLETKINNLSEELSVINTIKLESITHYHFDNVDLLITTHDIPKQTLNILPKHL
                     TTIKVAPLFSEDDRHKIRHVVKQKQNPVQAHHHMDTVNFLVVNTEQKSRHTVQIFEEA
                     QKILQAHHAIVEGYIESALEREKSSSTYIGNFMAIPHGDPEKVLQSHVLIFRTKDVFP
                     WRQHDVKLVFFLAISHKDKAFTKQMMQLIANLDDDSVNHLCSLDDHSLKQQLFEYLQE
                     "
     gene            2716..4668
                     /locus_tag="PPY92_001753"
     CDS             2716..4668
                     /locus_tag="PPY92_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HDJ2625746.1"
                     /translation="MKIVAITSCPNGIAHTYMAQEKLEQVAKEMGVDIKVETQGGVGA
                     ENVLTTQDIEEADGVIIAADKQVDLSRFVGKRLINENVREGIHNPRGLIQRIINQDAP
                     IYQSETNYHSKDRGKSKSGIQMVYQHLMNGVSFMVPFIVVGGLLIAIALTLGGETTSK
                     GLVIPDDSFWKSIENIGSLAFKFMVPILAGYIAVSIADKPGLVPGMIGGAIAADGSFY
                     GSDAGAGFLGGIVAGFLAGYIAKWIKDIKVPKAMAPIMPIIIIPIISSVVVGLIFIFL
                     IGAPISNIFEALTTWLKSMQGANIIILAMIIGAMIAFDMGGPVNKVAFLFGSALIAEG
                     NYAVMGMVAVAVCTPPIGLGLATFVQKYKFNHSEREMGKASFTMGLFGITEGAIPFAA
                     QDPLRIIPANIIGAMIASVIAAIGGVGDRVAHGGPIVAVLGGIDHVLWFIFAVIVGSL
                     VTMTTVLLLKRNTPVIAVDAPAQHTQLHDTDITQHDTEVDNVDKTAKHSHLNKPSHVF
                     DQQTMIITDHDMSRNEAIDMLIHKLKICRYVEHTSHLKNAILEREMESTTAIGMNVAI
                     PHAKSDVVKQPIVAVMKNNHGVKWDSLDGSLPQLIFLIAVPNNSQDTHLKILQRLSKA
                     LMNDETRQSLINANSTTEIYNLLMKI"
     gene            4682..5620
                     /gene="manA"
                     /locus_tag="PPY92_001754"
     CDS             4682..5620
                     /gene="manA"
                     /locus_tag="PPY92_001754"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDJ2625747.1"
                     /translation="MALFLQPVFKDRIWGGHALKAFNYDIPNETTGECWAISAHPNGP
                     NTIINGPYKDMTLDQLWSQHRELFDNDSRDSFPLLTKVLDANDKLSVQVHPDDDYALK
                     HEGELGKTECWYILDAAPGAEIIYGVHAHNKQTLIDMIDNHEFDSLFKRIPVKPGDFY
                     YVPAGTVHAIGSGILILETQQSSDTTYRIYDYDRRDQNGQLRDLHLEQSKDVIELGNH
                     EPNTTPITTHIDTHTVTQFVDNQYFAVYKWDIHGDLKFNKPHAYCLVTIIDGTGTLTV
                     NDKHYDIQKGSSFILTTEDEDIHFVGNISAIVSHPG"
     gene            complement(5732..8713)
                     /locus_tag="PPY92_001755"
     CDS             complement(5732..8713)
                     /locus_tag="PPY92_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDJ2625748.1"
                     /translation="MKNAFKLFKMDLKKVAKTPAVWIILAGLAILPSFYAWFNLWAMW
                     DPYGNTGHIKVAVVNEDKGDTIRGKKVNVGNTMVNTLKKNKSFDWQFVSREKADHEIK
                     MGKYFAGIYIPSKFTHEITGTLRKQPQKADVEFKVNQKINAVASKLTDTGSSVVVEKA
                     NEQFNKTVTRALLEEANKAGLTIEENVPTINKIKNAVYSADKALPKINDFANKIVYLN
                     NHQADLDKYANDFRKLGNYKGDILDAQKKLNEVNGAIPQLNEKAKLILALNNYMPKIE
                     KALNFAADDVPAQFPKINQGLNIASQGIDQANGQLNDAKGFVTQVRSRVGDYQDAIRR
                     AQDLNRRNQQQIPQNSAANNETSNSSPAAGNGVASTPPSAPSGDTAPNNNVTQNTAPN
                     SNNAPVSTTPQSTSGKKDGQSFADITTTQVSTANENTQNITDKDVKSMEAALTGSLLS
                     LSNNLDTQAKAAQKDSQALRNISYGILASDKPSDFRESLDNVKSGLEYTTQYNQQFID
                     TLKEIEKNENVDLSKEIDKVKAANNRINESLRLVNQLSNALKNGSSGTAEATKLLDQL
                     SKLDSSLSSFRDYVKKDLNSSLVSISQRIMDELNKGQTALSNVQSKLNTIDQVINSGQ
                     AILKNGKTRIDRLQTVLPSIEQQYISAVKNAQANFPKVKSDVAKAANFVRNDLPQLEQ
                     RLTNATASVNKNLPTLLNGYDQAVGLLNKNQPQAKKALSDLADFSQNKLPDVEKDLKK
                     ANKIFKKLDKDDAVDKLIDTLKNDLKKQAGIIANPINKKTVDVFPVKDYGSGMTPFYT
                     ALSVWVGALLMVSLLTVDNKHKSLESVLTTRQVFLGKAGFFIMLGMLQALIVSVGDLL
                     ILKAGVESPVLFVLITIFCSIIFNSIVYTCVSLLGNPGKAIAIVLLVLQIAGGGGTFP
                     IQTTPQFFQNISPYLPFTYAIDSLRETVGGIVPEILITKLIILTLFGIGFFVVGLILK
                     PVTDPLMKRVSEKVDQSNVTE"
BASE COUNT         2842 a         1578 c         1436 g         2863 t
ORIGIN      
        1 gcaatattga aataaattat ttagcttcac gtatcaacta cttttcatta acatggaggc
       61 gttatatttg aaaaaacttc ttattaccat cgtaattatc gtattaatcg gagcacttgg
      121 gttcgcaatt tatgctgcaa taaatcattc tacatcatca agtaataacc aagaacaaga
      181 aaataaatct acgcataaaa aaacagaaac cgaagaaaat gatcaacaag atgatagtgc
      241 agacaaaaca gataaaaatc aaaataatgg caataacgat actaatgaca atcaaccatc
      301 ttcaccgtca aatgttccta acaaccattc aacagtacca tcgaatacgc aaccagctca
      361 gccaaacgac tcaaattcaa ataaaaataa tactcacggt ggtggcagtc aatcgtcaac
      421 aaacaaccaa aataatcaac aacagaacac accacctgct aataaatcaa ataataccaa
      481 tacgccttct aaacaaacac ctcaagcaca atcacctaaa aattaatcat ttgcctgata
      541 cgtccacaag taccagatgc ataaattcag aaaaacagca gtgagatatt tttctatttg
      601 aaatatctta ctgctgttct ttatttgcat aattcgcact ctgttaaaat catataaaag
      661 ttcgctttaa tgtcattgac gtatgtataa gctctcatgt ttaataatat taatacacct
      721 ttaaaaacgc ttacacgaaa aatttagaaa ggaggtacat gttaaatgct tgatagacac
      781 ttaaaactac tccagttttt tattaaaaat ccatcaaagc acattagttc taatgaaatt
      841 gctgaacatg tcaatgtttc aaaccgcaca gttcgcaatg atattcatgt tatcaatagc
      901 aattttatgg atgatatcat cgtaagtatt aaatctaaag gatatcaatt aaatacatcg
      961 cagtatacct tagaaacgat aactgaacgt tacacgcata ttcaatcata taaagaaaaa
     1021 ctattattat ctatggcgta tcaactgcta atgcacaata aatcgcagac attgcaacaa
     1081 ctagagcaag attatctatt atctaaaact gtgctcaacg actatttcgt tcgaattcaa
     1141 caatggtgtc aaaaatttaa tatcgtttta acaatcaaga aaaaacaagg tattgtggtc
     1201 gatggaacag ataatgatat cactaatgcc atcatccatt taaatcaatt atcatcaggg
     1261 catgtacatg ttgaagacct aattttaaat gaattacctg attcacacca aaggatgatt
     1321 tcgcatatta tacaagagac acttcaacaa catagtattg aaacaactga tattcaaata
     1381 caacagcttt taattcatct cattttaatc attaaacgta agcaatcttt aagtgagcta
     1441 gacacgttaa accatgatgc aaaattgata tctcaatctt gcattaagca aatcaacgaa
     1501 agattaggtt atgacttgaa cgaaaaagta atcaatatgt tttcattctt tattgcttat
     1561 cattttaata aactcgatat tggtatagaa cgtatattta tacaaagtta tattgatcgc
     1621 ttaatcgaat tgatgcagca acgtattcat gtacctttca atgaagattc gattttacaa
     1681 caaaatttat ataatcattt tagtaaagca tatttacgta tcactcaaaa catttattta
     1741 aacaatcctt tagttataga gattaaaaaa ctctatccct ttgtgtttaa cacacttttt
     1801 gaagcgattg ataaattagc aattgatact gatatagaga tgagcgaaga tgaaattgca
     1861 tttttaacaa ttcattttca agctgccatc gagcgtcgta ccaaaacaca attaaatgta
     1921 gtcattgctt gttattatgg tctaggtgtg tcgaattttt tagaaactaa aataaataac
     1981 ttatccgaag agttgtcagt aataaatacg attaaactag aaagtataac acactatcac
     2041 tttgacaatg tagatttatt aataacgacc catgatattc caaaacaaac gctaaacata
     2101 cttcctaagc acctgacgac tatcaaggtt gcaccattat tttctgaaga tgatcgtcac
     2161 aaaatcagac acgttgtgaa gcaaaagcaa aatccggttc aagcacatca tcatatggac
     2221 actgtcaatt tccttgtcgt aaatactgaa caaaaatcac ggcatactgt gcaaattttt
     2281 gaagaagctc aaaaaatatt acaagcacat catgcgattg tagaaggata tatcgaatca
     2341 gctttagagc gtgagaaatc atcttctaca tacataggta attttatggc gattcctcat
     2401 ggagatcctg aaaaagtttt acaatcacat gtgcttattt ttcgcacaaa agatgttttt
     2461 ccttggcgac aacacgatgt caaacttgtc tttttcttag cgatttcgca taaagacaaa
     2521 gcttttacaa aacaaatgat gcaattaatt gctaacttgg atgatgattc agtgaaccat
     2581 ttatgtagtt tagatgatca tagtttaaag caacagttat ttgaatactt acaagaataa
     2641 ttgccactag taacggtaat tattcttttt ttagtatcga gaatacgctt acaatataga
     2701 aaaggagatg attttatgaa aattgtagct attacctcat gcccaaatgg cattgcacat
     2761 acatatatgg ctcaagaaaa gcttgagcag gtagcaaaag aaatgggtgt ggatattaaa
     2821 gtagagacac agggcggtgt tggtgctgaa aatgttttaa ctacgcaaga catcgaagaa
     2881 gctgacggtg tcataattgc agctgataaa caagttgatc tatcccgatt tgttggtaaa
     2941 cggttgatta atgaaaatgt tcgcgaaggg attcataatc cgagaggtct aattcaacgt
     3001 atcattaatc aagatgcgcc tatttatcaa tctgaaacaa attatcattc gaaagatcgc
     3061 ggtaagtcta aaagtggtat tcaaatggtg tatcaacatt taatgaacgg tgtatcgttt
     3121 atggttcctt ttatcgtagt tggtggactc cttatcgcca tcgcgctgac tctaggcggt
     3181 gaaacgacat caaaaggatt agtcatccca gatgattcat tttggaaatc cattgaaaac
     3241 attggtagtt tagcctttaa atttatggtt ccaatactag ctggatatat cgctgttagt
     3301 atcgctgaca agcccggtct tgttcctggt atgattggcg gcgctatcgc tgctgacggt
     3361 agtttttatg gcagtgatgc aggcgcagga tttttaggtg gtattgttgc tggtttctta
     3421 gcaggttata tcgcaaagtg gattaaagat attaaagttc caaaagcaat ggcaccaatt
     3481 atgcctatca ttattattcc cattatttca tcggtcgttg taggattgat atttatattt
     3541 ttaatcggtg caccaatttc taatattttt gaggcattaa caacctggtt aaaaagtatg
     3601 caaggtgcta atattattat acttgcgatg attataggtg cgatgatcgc cttcgatatg
     3661 ggcggtccag taaacaaggt agcattttta tttggctcag cacttattgc tgaaggtaat
     3721 tacgccgtta tgggtatggt cgcagttgct gtatgtacac caccaatcgg tttaggttta
     3781 gcaacgtttg ttcaaaagta taaattcaat cattccgaaa gagaaatggg taaggcttcc
     3841 ttcactatgg gactatttgg tattactgaa ggtgctattc ctttcgcagc ccaagatcca
     3901 ttgcgcatta tacctgcaaa catcattggt gcaatgattg catcagtcat tgcagccatt
     3961 ggtggtgtcg gcgatagagt cgcacatgga ggtccaatcg tggctgtatt aggtggtatt
     4021 gatcatgtat tatggtttat ctttgcagtg atagtaggta gtcttgtcac aatgactact
     4081 gtactattat taaaacgcaa tacaccagtt atagcagtag atgcaccagc gcaacacacg
     4141 caacttcatg atacagatat tacacaacat gatacagagg ttgacaatgt agataaaacc
     4201 gcgaaacatt cacatctcaa taaaccttct catgtttttg atcaacaaac tatgataatt
     4261 actgatcacg acatgtctcg aaacgaagca attgatatgt tgattcataa acttaaaatt
     4321 tgccgttatg tagagcacac atcgcattta aaaaatgcaa tactagaacg tgaaatggag
     4381 tctacgacag ctattggcat gaacgtggcc attccacatg caaagtctga tgttgtaaaa
     4441 caaccgattg tagctgttat gaaaaataat catggtgtta aatgggacag tctagatggt
     4501 agtctacctc aattgatatt cttaattgcc gtaccaaaca acagtcagga tacacattta
     4561 aaaatacttc agcgcttatc taaagcgtta atgaatgatg agacacgcca atcactaatt
     4621 aatgcaaaca gtactacaga aatttataac ttattaatga aaatatagag gagttttaac
     4681 tatggcatta tttttacaac ccgtttttaa agacagaatt tggggcggtc atgcattaaa
     4741 agcatttaac tatgatatcc ctaatgaaac aactggtgaa tgctgggcaa tttctgcaca
     4801 tccaaatggt cctaatacaa ttatcaatgg tccatacaag gatatgaccc tagatcaact
     4861 ttggtcacaa catcgtgaat tattcgataa tgattcacga gatagtttcc ctctgctaac
     4921 taaagtatta gatgccaatg ataaattatc tgttcaagtt catccagatg atgactacgc
     4981 tttaaaacac gaaggcgaac taggtaaaac agaatgttgg tatattttag atgcagcccc
     5041 aggtgctgaa attatatatg gcgtacatgc acataataaa cagacgttaa tcgacatgat
     5101 tgataatcat gaatttgaca gtcttttcaa acgtatacct gttaagcccg gtgactttta
     5161 ttatgttcct gctggcactg tccatgccat tggttcgggt attttaattt tagagacaca
     5221 acaatcatcc gatacgacat accgtattta tgactatgat agacgagatc aaaatggtca
     5281 attgcgtgat ttgcacttag agcaaagtaa agatgtgatt gaattaggca atcatgaacc
     5341 aaatactact cctataacaa ctcacatcga cacacacact gtaactcaat ttgtagataa
     5401 tcaatatttt gcagtatata aatgggatat tcatggagat ttaaaattca ataaaccaca
     5461 tgcttattgc ttagttacta ttatcgatgg tactggtact ttaacagtaa acgataagca
     5521 ctatgacatc caaaaaggtt ctagctttat tttaactacg gaagacgagg atattcactt
     5581 tgtcggtaat attagtgcta tagtaagtca tcctggataa tagcaatgat aacaaccgtc
     5641 ccataaaaga cagaaattac tatctcttct gtctaaaaac ataaaaatcc acacattaag
     5701 gagctataac cctaatgtgt ggatttaatt tttattctgt aacgttactt tggtcaactt
     5761 tttcagatac gcgcttcatc aatggatctg ttacaggttt taaaattaaa cctacaacga
     5821 agaatcctat gccaaataac gttaatataa ttaattttgt aattaggatt tccggaacaa
     5881 taccgcctac tgtttcacgt aatgaatcaa ttgcatacgt aaatggtaag tatggcgaaa
     5941 tgttttggaa aaattgtggc gtagtttgaa ttgggaatgt tcctccacca cctgcaattt
     6001 gtaatacaag caatacgatt gcaattgctt tacctgggtt accaagtaat gatacgcacg
     6061 tatatacgat tgagttgaaa ataatcgaac agaaaatcgt tataagtaca aataatacag
     6121 gtgactcaac tcctgctttt aggattaaca aatctccaac cgatacaatg agtgcttgca
     6181 acataccaag cattataaag aatcctgcct tacctaagaa cacttgtctt gtcgttaaga
     6241 ctgactctag actcttatgt ttattatcaa ccgttaataa acttaccatc aagagtgcac
     6301 ctacccatac tgacagtgca gtatagaatg gtgtcatacc tgaaccataa tccttaactg
     6361 ggaaaacatc aacagtcttc ttattaatag gatttgcaat aatacccgct tgctttttca
     6421 aatcattctt aagtgtgtcg attaatttgt cgactgcatc atctttgtct aatttcttga
     6481 aaattttatt cgcttttttc aaatcttttt caacatcagg caatttattt tgagaaaaat
     6541 cagctaaatc tgataaagcc tttttcgctt gtggctgatt tttatttagt aatcctaccg
     6601 cttgatcata accatttaat aacgttggta aatttttatt cacacttgct gtcgcattag
     6661 ttaatcgctg ttccaactgt ggtaagtcat tgcgcacaaa gttagcagct ttagctacat
     6721 cacttttcac tttcgggaag tttgcttgag catttttaac agcactaatg tattgttgtt
     6781 caatacttgg taatactgtt tgtaagcgat caatacgtgt tttaccattt tttaaaatag
     6841 cttgtccact gttaatgact tgatcaattg tatttaattt agactgaaca ttggataatg
     6901 cagtttgccc tttgttcaat tcatccataa tacgttgtga tattgatact aaagagctgt
     6961 taagatcttt tttaacataa tctctaaatg atgataatga tgaatctagt tttgaaagtt
     7021 gatctagtaa tttagtagct tcagcagttc ctgaactacc attctttaat gcattgctta
     7081 attgattaac taaccttaat gattcattaa ttcgattatt agctgctttt accttatcaa
     7141 tttcttttga taaatcaaca ttttcattct tctcaatctc ttttaatgta tcgataaatt
     7201 gttgattata ttgcgttgtg tattctaaac cggacttaac attatctaaa gactctctaa
     7261 aatcagaagg cttgtccgat gctaaaatgc catacgaaat attacgtaat gcctgactat
     7321 ctttttgtgc ggctttcgct tgggtatcta aattatttga taatgataat aaagagcccg
     7381 ttaatgccgc ttccattgat ttaacatctt tatctgtaat gttttgtgtg ttctcgttag
     7441 ctgtgctgac ttgtgttgtt gttatatctg caaaactttg accatctttt ttcccgcttg
     7501 tactttgtgg tgtagtcgat acaggcgcat tattactatt tggtgcggta ttttgcgtaa
     7561 cattattatt tggtgcagta tcgccacttg gtgcacttgg tggcgttgat gctacaccat
     7621 taccagctgc aggtgaacta tttgatgttt cgttgttcgc cgcgctattt tgaggaatct
     7681 gttgctggtt tcttcgattt aaatcttgcg cgcgtcgaat tgcatcttga taatcaccga
     7741 ctctacttct aacttgtgtg acgaagcctt tggcatcatt taactgtcca ttagcttgat
     7801 caatcccttg actcgcaatg ttaagtcctt gattaatttt agggaactgc gctggcacgt
     7861 catcagctgc aaaatttaac gctttttcaa ttttcggcat ataattattt aaagctaata
     7921 tcaacttagc cttttcatta agttgcggaa tagcaccatt gacttcgttt aattttttct
     7981 gagcatctaa aatatcacct ttataatttc ctagttttct aaaatcattg gcatatttat
     8041 ctaaatccgc ttggtggtta ttcaaataca caattttatt cgcaaagtca ttaatcttag
     8101 gtaaagcttt atctgctgaa tataccgcat tttttatctt gttaattgtc ggcacatttt
     8161 cttcaatagt taaacctgct ttgttagctt cttctaataa tgctcgagtt actgttttat
     8221 taaattgttc attcgctttt tcaacgacaa ctgacgaacc agtatctgtt agcttagacg
     8281 caacagcgtt aatcttctga ttcaccttaa attctacatc tgctttttga ggctgcttac
     8341 gtagtgtccc ggtaatttca tgtgtaaact tagatgggat gtaaatacct gcaaaatatt
     8401 tacccatttt tatctcatga tcagctttct ctctacttac aaactgccaa tcaaaacttt
     8461 tatttttctt gagtgtatta accatcgtat taccgacatt aacttttttc cctctgattg
     8521 tgtcgccttt atcttcatta acgactgcga ccttgatgtg tcccgtgttg ccatatggat
     8581 cccacattgc ccataagtta aaccaagcgt agaacgatgg caaaatagct aagcctgcta
     8641 agataatcca cacagctggc gtcttagcta ctttcttcag atccatttta aataatttaa
     8701 atgcgttctt cattgtcac
//
LOCUS       DANBWH010000111         8684 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.114, whole genome shotgun sequence.
ACCESSION   DANBWH010000111 DANBWH010000000
VERSION     DANBWH010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8684
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.114"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            86..1381
                     /locus_tag="PPY92_001756"
     CDS             86..1381
                     /locus_tag="PPY92_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625749.1"
                     /translation="MSFLRKHAEIIFSYLIGIVSLFTGLIILINLPLIKQLNGGKKVD
                     THVHNVWEFLNAFFSEIIKVMSRFIGNFPIVSAIVIIIFGILVMLIGHTLLRTIKYDY
                     DISIFFLVIGIMYFIITLILMTQVYGFFAVIFIIPFTIHIGYIVYKDELNQENVKNHF
                     MWIIVSYGISYLITQIALYGRIDANEIESIDILSVNAFFIIMWLLGQMAIWNFLFLRR
                     ALPLTKQELGEEEPELSRTSKGNVTNQTKIHLKQLQDKTTEYARKTRRSVDLDKIRAK
                     RDKFKKKVNDIIDIQEDDIPDWMRKPKWVKPMYVELFCGVVIFLFTFLEFNNRNALFV
                     SGDWKLSQTQYVIEWVTLLILLFIIIAYIATTLTFHLKGKFYYLQLFMGSILFFKLLT
                     EFINIMIHGLLLSVFITPTLLLMLLAIIISYSLQLRERP"
     gene            1806..2978
                     /locus_tag="PPY92_001757"
     CDS             1806..2978
                     /locus_tag="PPY92_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HDJ2625750.1"
                     /translation="MKIATLNKGKETKYFNGYPLIEEEDIYSQDHLKEGDIFQIVTDK
                     SQYVATAYVGRQHKGLGWVLTYDKAQEINTAFFVKLFNTALAERDYYFNIDGTNAFRL
                     FNAEGDGVGGLTIDNYDGHLLIQWYSKGIYKFKYAILEAVRKVFDYKSIYEKVRFKDS
                     EYSGGFVEGDAPEFPIVIEENFTFYNVDLEDGLMTGIFLDQKEVRKKLRGQYAKERHV
                     LNLFSYTGAFSVIAASEASSTTSVDLANRSRSLTEENFGLNAIDPKSQYIYVMDTFDF
                     YKYAARHGHSYDTIVIDPPSFARNKKRTFSVQKDYDKLINGALNILSSEGTLLLCTNA
                     SVYPLKQFKNTIKKTLEESGVDYELTEVMGLPKDFKTHPHYKPSKYLKAVFVNIRH"
     gene            3032..3574
                     /locus_tag="PPY92_001758"
     CDS             3032..3574
                     /locus_tag="PPY92_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HDJ2625751.1"
                     /translation="MGFKNNLTSNLTNKIGNSVFKIENVDGKGAMPTTIQELRERRQR
                     AEAIVKRKSLMSSTMSVVPIPGLDFGVDLKLMKDIIEDVNKIYGLDHKQVNSLGDDVK
                     ERIMSAAAIQGSQFIGKRISSAFLKIVIRDVAKRTAAKQTKWFPVVGQAVSASISYYF
                     MNKIGKDHIQKCENVIKNVM"
     gene            3728..3994
                     /locus_tag="PPY92_001759"
     CDS             3728..3994
                     /locus_tag="PPY92_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HDJ2625752.1"
                     /translation="MEQNSYVIIDETGIHARPATMLVQTASKFDSDIQLEYNGKKVNL
                     KSIMGVMSLGVGKDAEITIYADGSDESDAIQAISDVLSKEGLTK"
     gene            3997..5715
                     /gene="ptsP"
                     /locus_tag="PPY92_001760"
     CDS             3997..5715
                     /gene="ptsP"
                     /locus_tag="PPY92_001760"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDJ2625753.1"
                     /translation="MSKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAK
                     FNSAIEASKVELTKIRNNAEVQLGADKAAIFDAHLLVLDDPELIQPIQDKIKNENANA
                     ATALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVI
                     VGNDLTPSDTAQLNKEFVQGFATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGD
                     MIIVDGLNGDVIVNPTEDELIAYQDKRECYFADKKELQKLRDADTVTVDGVHAELAAN
                     IGTPNDLPGVIENGAQGIGLYRTEFLYMGRDQMPTEEEQFEAYKEVLEAMDGKRVVVR
                     TLDIGGDKELSYLNLPEEMNPFLGYRAIRLCLAQQDIFRPQLRALLRASVYGKLNIMF
                     PMVATINEFREAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDF
                     FSIGTNDLIQYTLAADRMSERVSYLYQPYNPSILRLVKQVIEASHKEGKWTGMCGEMA
                     GDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELANRAVDCATQEEVIEL
                     VNNYVK"
     gene            complement(5952..6185)
                     /gene="nrdH"
                     /locus_tag="PPY92_001761"
     CDS             complement(5952..6185)
                     /gene="nrdH"
                     /locus_tag="PPY92_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDJ2625754.1"
                     /translation="MSEIIVYTQNDCPPCTFVKNYLNEHQIDFEERNINNQQYRNEMI
                     DFDAFSTPFILLNGNPMYHVDLDEINKVLNIQD"
     gene            6383..7744
                     /locus_tag="PPY92_001762"
     CDS             6383..7744
                     /locus_tag="PPY92_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDJ2625755.1"
                     /translation="MDTVEISRFLTAMTLAVHIIFATIGVGMPLMFAIAEFLGIRKND
                     LQYIAMAKRWAKAYTITVAVGVVTGTIIGLQLSLIWPTFMEMGGHVIALPLFMETFAF
                     FFEAIFLSIYLYTWDRFKNKWTHFLISIPVIIGGSFSAFFITSVNSFMNTPAGFELKN
                     GKMVNVQPIEAMFNPSFIVRSFHVITTAGMTMAFVIASIAAFKLLRNRQPKDTVYHKK
                     ALKMSMIVGFFSTLLSMLAGDLSAKFLHKFQPEKLAAYEWHFDTSSHAKLLLFGVLDE
                     KTQQVKGAIELPGLLSFLADNSVKTKVQGLNDFPKSLHPPMIVHYFFDLMVTMGILCF
                     VISGVYVLTLMFKKLRKFSTHKWMLYGILLTGPASMLAIEFGWFLTEMGRQPWIVRGY
                     MRVAEAATQAGGITFVTILFGILYIILMYTCAYVLIRMFKNKPAYEDVNRLAKKQGGE
                     IEK"
     gene            7741..>8684
                     /locus_tag="PPY92_001763"
     CDS             7741..>8684
                     /locus_tag="PPY92_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDJ2625756.1"
                     /translation="MIYAFIGITVLWLFLFCYIIIASIDFGAGFFALHSKLTGDEKKI
                     NHLISRYLNPVWEVTNVFFVFFFVGFVGFFPESIKYLGTVLLVPGSIALIMISLRNSF
                     YAFENYGQDTKLAWMIMYGVSGLLIPASLSTALTITEGGYINVRNNVIDLDWVQLLLS
                     PFAWSVVFLAIISVLYISSGFLTYYAKKANDEPAYNLTRQWHIFLGPPMIIICLFVFL
                     SLRIQNSEHFYSAVFDYWWMFAISFLFFALASLLTFFKKKHGLAFVFVILQMMFAFFG
                     YGISKLPYLLYPFVKITDAYVNPEMGWTLVIVFILGLL"
BASE COUNT         2978 a         1154 c         1564 g         2988 t
ORIGIN      
        1 cttttctaag tctatagcac tatttattca tttatctaaa gacaacaaca ttagattaat
       61 atataatgat tttgaggtga acataatgtc ttttcttagg aaacacgccg aaattatttt
      121 tagctattta atcggtatcg tttcactctt cactggtctc attattttaa ttaacttgcc
      181 attaattaaa caattaaatg gtggtaaaaa agttgataca catgttcata atgtgtggga
      241 atttctgaat gcatttttca gtgaaattat taaagtaatg agtcgattta taggtaattt
      301 ccctatagtt agtgcaattg tgataattat attcggtatt ttagttatgt tgattggtca
      361 tacattactt agaactatta agtatgacta tgatatttct atctttttct tagttatcgg
      421 tatcatgtac tttattatta ctcttatatt aatgactcaa gtttatggat tctttgcagt
      481 gattttcatt attccattta caattcatat aggatatatc gtctataaag atgaattgaa
      541 tcaggaaaat gtaaaaaatc atttcatgtg gataattgtg agttatggta taagttactt
      601 aattacacaa attgcattgt atggcagaat tgatgctaat gaaatagagt caattgatat
      661 cttaagtgtc aatgctttct ttataattat gtggttactt ggtcaaatgg ctatttggaa
      721 tttcttgttc ttgcgccgag ctttaccttt aacaaagcaa gaattaggtg aagaggagcc
      781 agaattatca agaacaagta aagggaatgt cacgaatcaa actaaaattc acttgaaaca
      841 actccaagat aagactacag aatatgcacg taagacaaga agaagtgtcg atttagataa
      901 aattagagct aaaagagata aattcaaaaa gaaagttaat gatattatcg atattcaaga
      961 agacgatatt cctgattgga tgagaaaacc gaaatgggtt aaaccaatgt atgtcgaact
     1021 attttgtggt gtcgtcatct ttttattcac atttttagaa tttaataatc gtaatgcatt
     1081 atttgtatct ggtgattgga aattatcaca gacacaatat gttattgaat gggttacatt
     1141 attaattctg ttattcatta ttatcgcata tatcgctaca acgttaactt tccacttgaa
     1201 aggtaagttt tattatttac aactatttat ggggagcatt ttattcttta aattgttaac
     1261 ggaatttata aatataatga ttcatggact actactttca gtgttcatta cgccaacatt
     1321 actattaatg ttattggcaa tcatcatttc ttattcgtta caattacgag agcgaccata
     1381 attaaaagca ttataaaagt actatctatt aaacattttg atgtgtacgc tataagttag
     1441 atatatctct aacttattta gatacaggtc aatgaagttt atggatagta ctttttttgt
     1501 aactagattt gattgattca ggtgatgtga attaagtatt gaaaattgta tacaaagttt
     1561 tagtgcaaat aaaatagttg aaaagttatc catttgtaaa atcaagaaaa ctagtaaata
     1621 gttgaagcga catatggaat ttgcgaaacg atatatagta tttcctttgt agaaatttaa
     1681 catatatcat tcgaattact aatttgttaa aatcaacagt aagattagaa gtagatgata
     1741 ttgaaatttg gcaaacaatt taatctatat aaaactacaa cgaaacacag aaaggaagtt
     1801 gtcagatgaa aatagcaact ctgaacaaag gcaaagaaac aaaatatttt aatggatatc
     1861 ctttaattga agaagaggat atctattcac aagatcattt aaaagaagga gatatttttc
     1921 aaattgtgac tgataaatca caatatgttg caacggctta tgttggtcgc caacataaag
     1981 gattaggttg ggttctaaca tacgataaag ctcaagaaat caacacagct ttctttgtga
     2041 aattgtttaa tactgcatta gcagaacgtg attattattt taatatagat ggaacaaatg
     2101 cttttagatt atttaatgct gaaggtgatg gtgttggggg attaacaatc gacaattacg
     2161 atggtcattt gttgattcaa tggtactcaa aaggtattta taaatttaaa tatgccattc
     2221 ttgaagcggt tagaaaagta tttgattata aatctattta cgaaaaagta agatttaaag
     2281 acagcgaata tagtggtggt tttgttgaag gcgatgcacc tgagtttcca attgttatcg
     2341 aagaaaactt cacattttat aatgtagacc ttgaagatgg tttgatgaca ggtatctttt
     2401 tagatcaaaa agaagtgcgc aagaaattaa ggggtcaata tgccaaagaa cgccatgttt
     2461 taaacttatt tagttataca ggtgcttttt ctgtaatagc agcaagtgag gcatcttcaa
     2521 caacaagtgt agatttggct aatcgttctc gtagtttaac tgaagaaaat tttggattaa
     2581 atgctattga tcctaaatcc caatatattt atgtcatgga cacttttgat ttctataaat
     2641 atgctgcacg acatggacat agttatgaca cgatcgtgat tgatccacct agctttgcac
     2701 gcaacaaaaa acgtacattt tcagtgcaaa aagattatga caaattaatt aatggcgcct
     2761 taaatatttt atcatctgaa ggaacattat tgttatgtac aaatgcaagt gtatatccat
     2821 taaagcaatt taaaaatact attaaaaaga cgcttgaaga gagtggcgtt gattatgaat
     2881 taactgaagt tatgggttta ccaaaagatt ttaaaacgca tccacattat aagccatcta
     2941 aatatttaaa agctgttttt gtaaatatta gacattaatt tgataaaaag gggtatagta
     3001 acttattgag aaaaagaagg gtgataatat tatgggattc aaaaacaatt taacatcaaa
     3061 tttaacaaat aaaattggta attcagtctt taaaatagaa aatgttgacg gaaaaggtgc
     3121 aatgccaacg acgattcaag aattgagaga aagacgacaa cgtgctgaag caattgtaaa
     3181 gagaaagtct ttaatgtcat caacaatgag cgttgttcca attccgggtt tagattttgg
     3241 tgttgattta aaattaatga aagatattat cgaagatgta aataaaatat acggattaga
     3301 tcataagcaa gttaatagcc ttggggatga tgtgaaagaa agaattatgt ctgcagcagc
     3361 aattcaaggt agtcaattta ttggtaaaag aatttcaagt gcatttttaa aaatagttat
     3421 tagagatgta gctaaacgta ctgctgcaaa acaaacaaaa tggtttcctg ttgtaggaca
     3481 agctgtgtct gcatctatta gttactattt tatgaataaa attggaaaag atcacattca
     3541 aaaatgcgaa aatgttatta aaaatgtcat gtaggtgcta taatagtttt gcaatttgca
     3601 aattttactg aaaccggttt taaacgaatt gaatttaaag catggttttg gtaaagttaa
     3661 tgtataaaac taagttagta ttgtaataat attgaagatt ctaactatac gaaggagaaa
     3721 tgtaattatg gaacaaaatt catatgtaat catcgacgag actggtattc acgctagacc
     3781 agcaacaatg ttagtacaaa cagcttcaaa attcgattct gatattcaat tagaatataa
     3841 cggtaagaaa gtaaacttaa aatcaatcat gggtgttatg agccttggtg ttggtaaaga
     3901 tgctgaaatt acaatttatg ctgacggtag tgatgaatct gacgccattc aagcaatcag
     3961 tgacgtctta tcaaaagaag gattgactaa ataatcatgt ctaaattaat taaaggtatt
     4021 gctgcatctg atggtgtcgc aattgctaaa gcttatttat tagttgagcc agacttaaca
     4081 ttcgacaaaa atgaaaaagt cactgatgtt gaaggagaag ttgcaaagtt caatagcgct
     4141 atcgaagctt ctaaagttga gttaactaaa attagaaata atgcagaggt tcaactaggt
     4201 gctgataaag ctgctatctt tgatgcacat ttattagttt tagatgaccc tgaattaatt
     4261 caaccaatcc aagataagat taaaaatgaa aacgctaatg ctgctacagc attaacggat
     4321 gtaacaacac aatttgttac aatttttgaa tctatggata acgaatacat gaaagaacgt
     4381 gcggctgata ttcgcgacgt ttctaaacgt gtgttatcac atattttagg tgtagaatta
     4441 ccgaatccga gtatgattga tgaaagcgtt gttattgtag ggaatgactt aacgccatct
     4501 gatactgctc aattaaataa agaattcgta caaggttttg ctacaaacat tggcggaaga
     4561 acaagtcact ctgcaattat gagtcgttct ttagaaattc cagcaattgt tggtacaaaa
     4621 tcaattactc aagaagttaa acaaggcgac atgattatcg tagatggatt aaatggtgat
     4681 gtaatcgtta atccaactga agatgagtta atcgcttatc aagataaacg tgagtgttat
     4741 tttgctgaca agaaagaatt acaaaaacta cgtgatgctg atactgttac agttgatggt
     4801 gttcacgcag agcttgctgc aaatattggt acacctaatg atttgccagg tgttattgaa
     4861 aatggtgcac aaggtatcgg cttatataga actgagtttt tatatatggg tcgtgaccaa
     4921 atgcctacag aagaagaaca atttgaagct tataaagaag tattagaagc aatggacggt
     4981 aaacgtgttg ttgtacgtac tttagatata ggtggagata aagaattatc atacttaaac
     5041 ttgcctgaag aaatgaatcc attcttaggt taccgtgcga ttcgtttatg ccttgcgcaa
     5101 caagatattt tcagaccaca gctacgtgca ttattacgtg catcagttta tggtaagtta
     5161 aatatcatgt tcccaatggt tgcaacaatt aacgaattta gagaagcaaa agctatatta
     5221 ttagaagaaa aagaaaacct taaaaatgaa ggtcatgaca tttcggatga tatagaatta
     5281 ggaatcatgg tagagatacc tgcaacagca gcattagctg atgtctttgc taaagaagta
     5341 gatttcttca gtatcggtac aaatgattta attcaataca cattagctgc tgaccgtatg
     5401 tcagagcgtg tatcatatct ataccaacca tataaccctt caatcttacg tttagttaaa
     5461 caagttattg aagcgtcaca taaagaaggt aaatggacag gtatgtgtgg tgaaatggct
     5521 ggagatgaaa cagctattcc attattgctt ggtttaggtt tagatgagtt ctctatgagt
     5581 gcaacgtcta ttctgaaagc aagaagacaa attaatggtt taagtaaaaa tgaaatgact
     5641 gaacttgcta accgtgcagt cgactgtgca acgcaagaag aagttattga attagttaac
     5701 aactacgtaa aataattaat tctttcgaaa gacagaatta taaataaacc cgaagtattc
     5761 attgtgagta attatgaata cttcgggttc ttttttatga tttatgttaa tttcaatgga
     5821 atgttgttgc taactaatta aacaactatg tgtcattaac aaaatgttgt tctttaatta
     5881 atgttagtta atttaaaaat cgatatttaa tttatgatag ctataaatga tgaagttgat
     5941 ttagatgaaa attaatcttg gatatttaat actttgttga tttcatcaag atcaacatgg
     6001 tacattggat tgccattcaa caaaataaac ggagttgaaa aagcatcaaa atctatcatt
     6061 tcgtttcgat attgttgatt gttgatattt ctctcttcaa aatcaatttg atgctcattt
     6121 agataatttt ttacaaatgt acaaggtgga caatcattct gcgtataaac gattatttct
     6181 gacatgtatc atgctccttc gtgctttatt ctaatgtaat atacacaatt atacattgaa
     6241 attcaaattt gtgaacacat tgtgaactga cataaattat acacaatttc atagcgcata
     6301 atgttttaaa aatacacatt atagcaatta taattaagtt cattgtgttg tcatcaaaaa
     6361 aagaaaaagg tgatgttttt aaatggatac agttgaaatc agtcggtttt tgacagctat
     6421 gactttagca gttcatatca tttttgcaac gattggtgtt ggtatgcctt taatgttcgc
     6481 aattgcagaa tttttaggta ttcgcaaaaa tgatcttcaa tatatagcta tggccaaaag
     6541 atgggctaaa gcttatacaa ttactgtagc agtgggagtt gttacaggta caattatagg
     6601 acttcaatta tcattgattt ggcctacatt tatggaaatg ggtggacacg ttattgcact
     6661 tccattattt atggaaacat ttgcgttctt ctttgaagct attttcttaa gtatatattt
     6721 atatacttgg gatcgtttta aaaataaatg gacacatttc ttaattagta taccagtaat
     6781 tattggtggc tctttctcag ccttcttcat tacttcagtg aattcattta tgaatacgcc
     6841 tgcaggtttt gagttgaaga atggaaagat ggtcaatgtt caacctatag aagcgatgtt
     6901 taacccatcg tttatagttc gatcatttca cgtaattact acagccggta tgacgatggc
     6961 atttgttatt gcatcaatag cagcttttaa attattgcgt aatcgtcaac ctaaagatac
     7021 tgtctaccat aagaaagctt tgaaaatgtc tatgattgtt ggattctttt caactttact
     7081 ttctatgttg gcaggggatt tatctgcaaa atttttgcat aaattccaac ctgaaaaatt
     7141 agcagcttat gaatggcatt tcgatacatc ttcccatgct aaattattat tatttggtgt
     7201 gttagatgaa aagactcagc aagttaaagg tgcgattgaa ttacctggac tattgagttt
     7261 cttagcagat aatagtgtca aaactaaagt gcaagggtta aatgattttc caaaaagttt
     7321 acatccgcct atgattgtcc attatttctt tgatttaatg gtaacgatgg gaattttatg
     7381 ttttgtcatt tcaggtgttt atgttttaac tttaatgttt aaaaagctta gaaagttttc
     7441 tactcataaa tggatgcttt acggaatatt attaacagga ccagcttcaa tgctagctat
     7501 agaatttgga tggttcttaa cagagatggg tagacagcct tggattgttc gtggttatat
     7561 gcgcgtggca gaagcagcaa cacaagcagg cggaataacc ttcgttacaa ttttatttgg
     7621 catattgtac atcattttaa tgtatacatg tgcatacgta ttaattcgta tgtttaaaaa
     7681 taaaccggcg tatgaagatg taaatcgttt agccaagaag caaggaggag aaatagaaaa
     7741 atgatttatg catttatagg tataacagtg ttatggttgt ttttattttg ctatatcatt
     7801 attgcttcta tagattttgg ggcaggtttc ttcgcattgc attcaaagtt aactggtgat
     7861 gaaaagaaaa ttaatcactt aatttcacgt tatttgaacc cagtttggga agttacgaat
     7921 gtattctttg ttttcttctt cgtaggattc gtaggtttct ttccagagtc aatcaaatat
     7981 ttaggcacgg tattgttagt accaggttca atagcactga ttatgatatc attgagaaac
     8041 agtttttatg catttgaaaa ttatggtcaa gatacaaaat tagcatggat gatcatgtat
     8101 ggggtaagtg gattattaat tccagcttca ttatctactg ctttaactat tacagaaggt
     8161 ggctatatta atgttcgaaa caatgttatc gatctagatt gggtgcagtt actattaagc
     8221 ccatttgctt ggtctgtagt attcttggca attatttcag ttttatatat ttcatcagga
     8281 tttttgacat attatgctaa aaaagcaaat gacgaaccag catataattt aacaagacaa
     8341 tggcacatat ttttagggcc accgatgatt atcatttgtt tattcgtatt tctatcatta
     8401 cgcattcaaa attctgaaca tttttattca gctgtttttg actactggtg gatgtttgct
     8461 ataagtttct tattctttgc attagcttca ttgttaacat tctttaagaa aaaacatggt
     8521 ttggcttttg tatttgttat tttacaaatg atgttcgcgt tctttggcta tggaattagt
     8581 aaattgccat accttttata tccgtttgta aaaattacag atgcatatgt taatccagaa
     8641 atgggctgga cattagtgat tgtctttatt ttaggtttac tttt
//
LOCUS       DANBWH010000112         8563 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.115, whole genome shotgun sequence.
ACCESSION   DANBWH010000112 DANBWH010000000
VERSION     DANBWH010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8563
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.115"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(52..678)
                     /locus_tag="PPY92_001764"
     CDS             complement(52..678)
                     /locus_tag="PPY92_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDJ2625757.1"
                     /translation="MAKVLYITAHPFNELVSNSMAAGKAFIETYQQQHPDDEVKHIDL
                     FETYIPVIDKDVLTGWGKMSNGETLTDDEQMKVSRLSDILEEFLSADKYVFVTPMWNL
                     SFPPVVKAYIDAISIAGKTFKYSAEGPQGLLTDKKVLHIQSRGGYYTEGPAADFEMGD
                     RYLRTIMTFLGVPSYETIIIEGHNAEPHKTEEIKATSINNAEKLATTF"
     gene            887..1465
                     /locus_tag="PPY92_001765"
     CDS             887..1465
                     /locus_tag="PPY92_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HDJ2625758.1"
                     /translation="MTKRPKRILATIIIFLSLLFTIIYIDDIQKWFNQYTDKLTQNHK
                     GQGHSKWEDFFRGSRITETFGKYQHSPFDGKHYGIDFALPKGTPIKAPTNGKVTRIFN
                     NELGGKVLQIAEDNGEYHQWYLHLDKYNVKVGDRVKAGDIIAYSGNTGKQTTGAHLHF
                     QRMKGGVGNAYAEDPKPFIEQLPDGERSLYDL"
     gene            1849..2946
                     /gene="ugpC"
                     /locus_tag="PPY92_001766"
     CDS             1849..2946
                     /gene="ugpC"
                     /locus_tag="PPY92_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDJ2625759.1"
                     /translation="MAELKLEHIKKTYDNNNTVVKDFNLHITDKEFIVFVGPSGCGKS
                     TTLRMVAGLESITSGDFYIDGERMNDVEPKNRDIAMVFQNYALYPHMTVFENMAFGLK
                     LRKVNKKEIEQKVNEAAEILGLTEYLGRKPKALSGGQRQRVALGRAIVRDAKVFLMDE
                     PLSNLDAKLRVQMRTEILKLHKRLNTTTIYVTHDQTEALTMASRIVVLKDGDIMQVGT
                     PREIYDAPNCIFVAQFIGSPAMNMLNATVEMDGLKVGTHHFKLHNKKFEKLKAAGYLD
                     KEIILGIRAEDIHEEPIFIQTSPETQFESEVVVSELLGSEIMVHSTFQGMELISKLDS
                     RTQVMTNDKITLAFDMNKCHFFDEKTGNRIV"
     gene            2959..4230
                     /locus_tag="PPY92_001767"
     CDS             2959..4230
                     /locus_tag="PPY92_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDJ2625760.1"
                     /translation="MSKILKCITLAVVMLLIVTACGPNRSKEDIDKALNKDNSKDKPN
                     QLTMWVDGDKQMAFYKKITDQYTKKTGIKVKLVNIGQNDQLENISLDAPAGKGPDIFF
                     LAHDNTGSAYLQGLAAEIKLSKDELKGFNKQALKAMNYDNKQLALPAIVETTALFYNK
                     KLVKNAPQTLEEVEANAAKLTDSKKKQYGMLFDAKNFYFNYPFLFGNDDYIFKKNGSE
                     YDIHQLGLNSKHVVKNAERLQKWYDKGYLPKAATHDVMIGLFKEGKVGQFVTGPWNIN
                     EYQETFGKDLGVTTLPTDGGKPMKPFLGVRGWYLSEYSKHKYWAKDLMLYITSKDTLQ
                     KYTDEMSEITGRVDVKSSNPNLKVFEKQARHAEPMPNIPEMRQVWEPMGNASIFISNG
                     KNPKQALDEATNDITQNIKILHPSQNDKKGD"
     gene            4233..5501
                     /locus_tag="PPY92_001768"
     CDS             4233..5501
                     /locus_tag="PPY92_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDJ2625761.1"
                     /translation="MTKRNPKLAALLSVIPGLGQFYNKRPIKGTIFFIFFISFISVFY
                     SFLNIGFWGLFTLGTVPKLDDSRVLLAQGIISILLVAFAIMLYVINILDAYRNAERFN
                     RNEEIKDPKARMVATWDKTFPYLLISPGTFLLIFVVVFPLIFMFGVAFTNYNLYNAPP
                     RHTLEWVGLDNFKTLFTIGVWRKTFFSVITWTLVWTLVATTLQIALGLFLAIIVNHPV
                     VKGKKFIRTVLILPWAVPSFVTILIFVALFNDEFGAINNDILQPLLGVAPAWLSDPFW
                     AKVALIGIQVWLGFPFVFALFTGVLQSISSDWYEAADMDGASSWQKFRNITFPHVIYA
                     TAPLLIMQYAGNFNNFNLIYLFNKGGPPVSGQNAGSTDILISWVYNLTFEFNNFNMGA
                     VVSLIIGFIVAIVAFIQFRRTSTFKDEGGL"
     gene            5503..6342
                     /locus_tag="PPY92_001769"
     CDS             5503..6342
                     /locus_tag="PPY92_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDJ2625762.1"
                     /translation="MTKKKNILKAIGIYSFIAMMFVIILYPLLWTFGISLNPGTNLYG
                     AKMIPDNATFKNYAFLLFDDSSQYLTWYKNTLIVASANALFSVIFVTLTAYAFSRYRF
                     VGRKYGLITFLILQMFPVLMAMVAIYILLNTIGLLDSLFGLTLVYIGGSIPMNAFLVK
                     GYFDTIPKELDESAKIDGAGHMRIFLQIMLPLAKPILAVVALFNFMGPFMDFILPKIL
                     LRSPEKFTLAVGLFNFINDKYANNFTVFAAGAIMIAVPIAIVFLFLQRYLVSGLTTGA
                     TKG"
     gene            6416..7492
                     /locus_tag="PPY92_001770"
     CDS             6416..7492
                     /locus_tag="PPY92_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDJ2625763.1"
                     /translation="MTIKVGIIGCGGIANGKHMPSLQKVENVEMIAFCDVDISKAASA
                     AEAYGTDNAKVYDDYKALLKDDTIDVIHVCTPNDSHCEITVAGLHAGKHVMCEKPMAK
                     TTAEAQKMIDTAKSTGKKLTIGYQNRFRPDSQFLHQAAQRGDLGDIYFGKAHAIRRRA
                     VPTWGVFLNEEAQGGGPLIDIGTHALDLTLWMMDNYEPESVMGSTFHKLNKQHDAANA
                     WGSWNPDEFTVEDSAFGFIKMKNGATIILESAWAINSLEVDEAKCSLSGTKAGADMKD
                     GLRIHGEDMGTLYTKHVELENKGVDFYEGNEVDEAEEEAKAWIDAVVNDTEPVVKPEQ
                     AMVVTKILEAIYQSAKSGKAIYFE"
     gene            7517..8557
                     /locus_tag="PPY92_001771"
     CDS             7517..8557
                     /locus_tag="PPY92_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDJ2625764.1"
                     /translation="MTKLKVGVIGVGGIAQDRHIPALLKLKDTVSLVAVQDINTVQMI
                     DVAKRFNIPQAVETPSELFKLVDAVVICTPNKFHADLSIEALNHGVHVLCEKPMAMTT
                     EECDRMIEAANKNHKLLTVAYHYRHTDVAMTAKKAIEAGVVGKPLVARVQAMRRRKVP
                     GWGVFTNKALQGGGSLIDYGCHLLDLSLWLLGKDMVPHEVLGKTYNQLSKQPNQINDW
                     GTFDHTKFDVDDHVTSYMTFANRASMQFECSWSANIKEDKVHVSLSGEDGGINLFPFE
                     IYEPRFGTIFESKANVEHNEDIAGERQARNFVNACLGIEEIVVKPEEARNVNALIEAI
                     YRSDLDNKSIQL"
BASE COUNT         2888 a         1322 c         1709 g         2644 t
ORIGIN      
        1 tacaataaaa aactgcatga cgttgaggag tcacacagtg taattaaaaa tttaaaaagt
       61 tgttgctaat ttttcagcat tattaatact agttgcttta atttcttcag ttttatgcgg
      121 ttcggcattg tgtccttcaa taatgattgt ttcatatgat ggcacaccta agaatgtcat
      181 aattgttctt aaataacggt cacccatttc aaaatcagca gcaggtcctt cagtataata
      241 tccaccacgt gattgaatgt gtaatacttt tttgtcagtt agtaaacctt gtggtccttc
      301 agcagaatat ttaaaagttt tacctgcaat tgaaatagca tcaatatatg ctttaactac
      361 aggtgggaaa gaaaggttcc acataggcgt tacaaataca tatttatctg cacttaaaaa
      421 ttcttctaaa atgtcactca atcttgaaac tttcatttgt tcatcatcag ttaacgtttc
      481 gccattactc atttttcccc aaccagttaa tacatctttg tcaataactg gaatataagt
      541 ttcaaataaa tcaatatgtt tcacttcatc atcaggatgt tgttgttgat atgtttcgat
      601 aaatgcttta ccagccgcca tagaatttga taccagttca ttaaaagggt gtgctgtaat
      661 atataatact tttgccattt gaaaattctc ctctgtttct gttattttct taagtataat
      721 tattatactc gatataaaat ttaatatcaa tcaaaatatt caaattacca tcattttctt
      781 catctatatt tggcagtact actaaagtat gagtgcattt aattatgaaa tagttgattt
      841 agaatatata cttaataccc aaaatatatg aaggatggat gccactatga caaagcgacc
      901 aaaacgtatt ttggcaacaa ttatcatttt tctttcacta ttatttacga ttatttatat
      961 agatgacatt caaaaatggt ttaaccaata taccgataaa ttgacacaaa atcataaagg
     1021 acaaggacac tcaaaatggg aagacttttt tagagggagt aggattactg agacttttgg
     1081 caaatatcaa cattcaccat ttgatggtaa gcattatggc attgattttg cattgccaaa
     1141 aggtacacca attaaagcgc cgacgaatgg taaagtaaca cgtatcttta ataatgaatt
     1201 aggcggcaag gtattacaga ttgccgaaga caatggagaa tatcaccagt ggtatctaca
     1261 cttagacaaa tataatgtca aagtaggtga tcgagttaaa gcaggtgata ttattgcata
     1321 ttcaggcaat acaggtaaac aaacgacagg cgcacattta cattttcaaa gaatgaaggg
     1381 tggcgtaggt aatgcatatg cagaagatcc taaaccgttt atcgagcagt tacctgatgg
     1441 ggaacgtagc ctatatgatt tgtagttata gaagagtgcc cgcagtctaa aaaattaagc
     1501 aatcattgtg tgagtatgat acttacataa tggttgcttt tttcaatgaa aatcgtaatg
     1561 ctaagtcata cttgtttgat ttagatatta cttaaaatgt aagacaaggt tgttagcatt
     1621 ggcagtgaaa tatcgcacat aaaaaaacat tattgtcaca ctagaaaata gttgtgtact
     1681 atatcaattt tctgtataaa agtttaattc tgacagtaat gtaaacgttt acaatttatg
     1741 attgacatta ataatgactg aatatatgat ttatgtaagt atttgtgcaa acgttttcac
     1801 aaagtgtatt gcacaatcaa actgtaaaca aagtatggga ggcataacat ggcagaacta
     1861 aagttagagc atattaaaaa gacgtatgat aacaacaata ctgtagtgaa agattttaat
     1921 ctacatatta ctgacaaaga attcattgta tttgttggac catcgggatg tggtaaatca
     1981 acaacattac gaatggttgc tggactagag tctatcacat ctggagattt ttatattgat
     2041 ggggaacgca tgaacgatgt tgaaccaaag aatagagata ttgcgatggt atttcaaaac
     2101 tatgcattat atccacatat gactgtattt gaaaatatgg catttgggct aaagctacgt
     2161 aaagtaaata aaaaagagat tgaacaaaaa gtcaatgaag cagctgaaat attaggatta
     2221 actgagtatc ttggtcgtaa accaaaagcg ttatctggtg gacagcgtca acgtgttgct
     2281 ttgggcagag ctattgttag ggatgcgaaa gtctttttaa tggatgaacc attatcgaat
     2341 cttgatgcga agcttcgagt acaaatgcgc acagaaatat tgaaattaca taagcgactt
     2401 aatactacga caatttatgt tacacatgat caaactgaag cattgacgat ggctagtcga
     2461 attgttgttt tgaaagatgg cgacattatg caagtcggca cacctagaga aatatatgat
     2521 gcccctaatt gcatatttgt ggcgcaattt atcggctcac cagcaatgaa tatgttgaat
     2581 gctacagttg aaatggacgg attgaaggta ggaacacatc attttaaatt acataataaa
     2641 aaatttgaaa agttaaaagc tgctggctac ttagacaagg aaattatttt aggtattcga
     2701 gctgaagaca ttcatgaaga accaatattt attcaaactt ctccagagac acaatttgaa
     2761 tctgaagtag ttgtatccga gttgttaggt tcagaaatca tggtacatag tacattccaa
     2821 ggaatggaat tgatttctaa attagattca agaacccaag tgatgacgaa cgacaagatt
     2881 acactagcat ttgatatgaa taagtgtcac ttttttgatg aaaaaacagg aaatcgtatc
     2941 gtctaagggg gagtattcat gtctaaaatt ttaaaatgta tcacgttagc cgtggtaatg
     3001 ttattaatcg taactgcatg tggccctaat cgttcgaaag aagatattga taaagcattg
     3061 aataaagata attctaaaga caagcctaac caacttacga tgtgggtgga tggcgacaag
     3121 caaatggcgt tttataaaaa aattacggat caatatacta aaaaaactgg catcaaagta
     3181 aagcttgtaa atattggtca aaatgatcaa ctagaaaata tttcgctaga cgctcctgca
     3241 ggaaaaggtc cagatatctt tttcttagca catgataata ctggaagtgc ctatctacaa
     3301 ggcttagctg ctgaaatcaa attatcaaaa gatgagttga aaggtttcaa taagcaagca
     3361 cttaaagcga tgaattatga caataagcaa ctagcattgc cagctatcgt agaaacaacc
     3421 gcacttttct ataataaaaa attagtgaaa aatgcaccgc aaacgttaga agaagttgaa
     3481 gctaatgctg ccaagctaac tgatagtaaa aagaaacaat acggtatgtt atttgatgct
     3541 aaaaatttct attttaatta tccgttttta ttcggcaatg atgattatat tttcaagaaa
     3601 aatggcagtg aatatgatat tcatcagcta ggactaaatt caaaacatgt cgtcaaaaat
     3661 gctgaacgat tacaaaaatg gtacgacaaa gggtatcttc ctaaggcagc aacacatgat
     3721 gtcatgattg gtctttttaa agaaggaaaa gtaggacaat ttgtcactgg accgtggaac
     3781 attaatgaat atcaagaaac gtttggtaaa gatttaggag taacaacatt acctacagat
     3841 ggtggcaaac ctatgaaacc atttctcggt gtacgtggtt ggtatttatc tgaatatagt
     3901 aaacataagt attgggctaa agatttaatg ctgtatatca ctagtaaaga tacattacaa
     3961 aaatatacag atgaaatgag cgaaattact ggacgtgttg acgtgaaatc atctaatcca
     4021 aatttaaaag tgtttgaaaa gcaagcacgt catgctgaac cgatgcctaa tattcctgaa
     4081 atgcgacaag tttgggaacc gatgggcaat gcaagcatat ttatctcaaa tggtaagaat
     4141 cctaaacaag cgttagatga ggcgacgaat gatataacgc aaaatattaa gattcttcat
     4201 ccatcacaaa atgataagaa aggagattag ttatgacgaa acgtaaccct aaattagcgg
     4261 cattattatc tgttatacct ggtttgggac agttttataa taaaagaccc attaaaggta
     4321 cgatattttt tatctttttc atcagtttta tttctgtatt ttatagcttt ttaaatattg
     4381 gtttttgggg attgttcaca ttagggacag tacctaagtt agacgattct cgtgtcttac
     4441 ttgcacaagg tattatttct atcttactcg ttgctttcgc aatcatgcta tatgtcatta
     4501 atattttaga tgcatatcgt aatgctgaac gatttaatcg caatgaggaa ataaaggatc
     4561 cgaaggcgcg tatggtggca acatgggaca agacgttccc atacttacta atctcaccag
     4621 gtacattctt attgatattt gtagttgtat ttccattaat atttatgttt ggagtagcat
     4681 ttacaaacta caatttatac aacgcgcctc cgagacacac attagaatgg gttggtttag
     4741 ataactttaa aacgttattc acaattggcg tttggcgcaa aacatttttc agtgttatta
     4801 cttggacatt agtatggacg cttgttgcaa cgacacttca aattgcatta gggctgtttt
     4861 tggcaattat tgtaaatcac cctgtcgtca aaggtaagaa atttatccgt actgtgttaa
     4921 tcctaccttg ggctgtacca tcatttgtga caattttaat atttgtagcg ttatttaatg
     4981 atgaatttgg tgcgataaat aatgatattt tgcaaccttt attaggtgta gcaccagcat
     5041 ggttaagtga tccgttttgg gcaaaagtgg cattaatcgg cattcaagta tggcttggat
     5101 tcccatttgt ctttgcactg ttcactggag tactgcaaag tatttcatca gattggtacg
     5161 aagcagcaga tatggatggt gcgtctagtt ggcaaaagtt tagaaacatc acattcccgc
     5221 atgtcattta cgccacagcg ccattgttaa ttatgcaata tgcaggtaat ttcaataatt
     5281 ttaatcttat ttatctattt aataaaggcg gtccaccagt gtcagggcag aatgctggta
     5341 gtacagatat cttgatatct tgggtgtata atctgacatt tgagtttaac aacttcaaca
     5401 tgggtgcagt tgtgtcatta attattggat ttattgttgc tattgtcgca tttattcaat
     5461 tcagacgtac aagtacgttt aaagatgagg gaggtttata agatgacaaa gaagaaaaac
     5521 atattaaaag caatcggtat ttacagtttt atagcgatga tgtttgtcat cattttatat
     5581 ccactactgt ggacatttgg catttccctt aatccaggta cgaacttgta tggtgccaaa
     5641 atgataccag acaatgcaac atttaaaaat tatgcattct tactattcga tgacagtagt
     5701 caatacctga cttggtataa aaatacgctt atcgtagcat ctgcaaatgc actgtttagt
     5761 gtgatatttg tcacgttaac agcatatgct ttttctagat atcgctttgt tggtcgtaaa
     5821 tacgggctga ttacattttt gattttacaa atgttccctg tattaatggc aatggtcgca
     5881 atctatattt tgctaaatac aattggttta ttagattctt tatttggact aacactggta
     5941 tatattggtg gatcaatacc gatgaatgcc tttttagtga aaggttactt cgatacgatt
     6001 ccaaaagaac ttgatgaatc tgccaaaatt gatggtgcag ggcatatgcg tattttctta
     6061 caaattatgc ttccattagc taagccgatt ttagcagttg ttgctttgtt caattttatg
     6121 ggaccattta tggactttat attacctaaa atactattaa gaagtcctga aaaattcaca
     6181 ttagcagttg gattgttcaa ctttattaat gataagtatg caaataattt cacagtgttt
     6241 gcagcagggg caattatgat tgcagtacct atagcaatcg tattcttgtt cttgcaacgc
     6301 tatttagtat caggtttaac aacaggtgcg acaaaaggtt agtttgaaat taggagtggg
     6361 gcagaattga taaagaacca ctaatgacga taaagattaa aaggaggacg ttatgatgac
     6421 gattaaagtt ggaatcattg ggtgtggtgg tattgcgaat ggcaagcaca tgccaagttt
     6481 acaaaaagtt gaaaatgttg aaatgatcgc attttgtgac gtagacattt cgaaagcagc
     6541 gagtgcggca gaagcatacg gaactgacaa tgcaaaggtt tatgatgatt acaaagcatt
     6601 gttaaaagat gacacgattg atgttatcca tgtttgtacg ccaaatgact cgcattgtga
     6661 aattactgta gcagggttgc atgccggtaa gcatgtgatg tgtgaaaaac caatggctaa
     6721 aacgacagca gaagctcaaa aaatgataga tacagctaaa tcaacaggta aaaaattaac
     6781 aataggttat caaaatcgct tcagaccaga tagtcaattt ttacatcaag cagcgcaacg
     6841 tggcgactta ggagacattt acttcggaaa ggcacatgcc attcgtcgtc gtgcagtacc
     6901 gacatggggt gtctttctaa acgaagaagc ccaaggtgga ggacctttaa tcgatattgg
     6961 tacgcacgca ttagatttaa cgttatggat gatggataat tatgaaccag aatcagtgat
     7021 gggttcaaca ttccataaat taaataagca gcatgatgcg gcaaacgctt ggggttcatg
     7081 gaatccagat gaatttacag ttgaagactc tgcgtttggc tttattaaaa tgaagaacgg
     7141 agcgacgatc attttagaat ccgcttgggc gattaattct ttagaagtgg atgaggcaaa
     7201 atgttcatta tcaggaacga aagcaggtgc tgatatgaaa gatggtctac gtattcatgg
     7261 tgaagatatg ggcacacttt ataccaaaca cgttgaattg gaaaacaaag gcgtcgactt
     7321 ttatgaaggt aatgaagtgg atgaagctga agaagaagca aaagcttgga ttgatgcagt
     7381 tgtaaatgat actgaaccag ttgtgaaacc ggaacaagca atggtagtta caaaaattct
     7441 tgaagcgatt tatcagtctg caaaatcagg caaagcaatt tactttgaat aacatcatac
     7501 ggtaaggagg cacatcatga caaaattaaa agttggtgtg ataggtgttg gtggcattgc
     7561 acaagaccgt catattccag cattgctgaa actcaaagac acagtctcat tagttgcagt
     7621 acaagatatt aatacagtgc agatgattga tgttgcgaag cgctttaata tacctcaggc
     7681 agttgagaca cctagcgagc tgtttaaact tgttgatgcg gtggtcattt gtacacccaa
     7741 taaattccat gctgatcttt ctatagaagc attgaaccat ggtgtccatg tcttatgcga
     7801 aaagccaatg gcaatgacga cggaagagtg tgatcgcatg attgaagcgg ctaataaaaa
     7861 tcacaaatta ttaactgtcg cttatcatta tcgtcacaca gatgtcgcta tgactgctaa
     7921 aaaagcaatt gaagcaggtg tggttggtaa accattagta gcacgtgtac aagcgatgcg
     7981 taggcgtaaa gtacctgggt ggggcgtttt taccaataaa gcgttgcaag gtggcggtag
     8041 tttaatcgat tatggttgcc acttgttaga cttatctttg tggctattag gtaaagatat
     8101 ggtgccgcat gaagtgctag gaaaaacata taatcaattg agcaaacaac cgaatcaaat
     8161 taatgattgg ggaacatttg atcacactaa atttgatgtc gatgatcatg ttactagtta
     8221 tatgacattt gccaatcgag caagcatgca gtttgaatgt tcgtggtctg caaatataaa
     8281 ggaagataag gtacacgtta gtttatcagg agaagatggc ggtatcaatt tatttccatt
     8341 tgaaatatat gagccccgct ttggaactat ttttgaaagc aaagctaatg ttgagcataa
     8401 cgaagacatt gctggtgaga gacaggcgcg taactttgtc aatgcgtgtt taggtataga
     8461 agagattgtg gtgaaaccgg aagaagcacg caatgtaaat gcccttatag aagcgattta
     8521 tcgtagcgat cttgataaca agagcataca actttaatga tta
//
LOCUS       DANBWH010000113         8539 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.116, whole genome shotgun sequence.
ACCESSION   DANBWH010000113 DANBWH010000000
VERSION     DANBWH010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8539
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.116"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            347..1488
                     /locus_tag="PPY92_001772"
                     /pseudo
     CDS             347..1488
                     /locus_tag="PPY92_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558285.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSau4 family transposase"
     gene            1739..2785
                     /locus_tag="PPY92_001773"
     CDS             1739..2785
                     /locus_tag="PPY92_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625765.1"
                     /translation="MNNKDIYSDDFQNALVDIILNIDKINVKEVFDDSCRAVDQDNKK
                     DTLNRSPKDFSINISDIVRYSILCMENLSSDKIEENINMIKNHSSELNDNDMFIQEKN
                     NFTTNFNKFCNALGINIANFKSGKSYVFKPKSLFVLYDILSNNSVYLHFFKNGINDKD
                     IDSSNRIKENLYYFINHEITDQRDYEHALTNYYLYFLVDSNIEIGIKSIIIKIFTEKG
                     ITSKDKLQILEYYLDEIDDLNTEISTKIADAHVKNSTNFLENEKVSINSNLAKYITHR
                     DTRASAPKNMLLSLILNSDLVSPKDLEEKELKYPKKITKEHHDKLIKEINKALDYYND
                     NNTTPSDYFYITSD"
     gene            2978..3274
                     /locus_tag="PPY92_001774"
     CDS             2978..3274
                     /locus_tag="PPY92_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDJ2625766.1"
                     /translation="MYFNQSDKFEQLKKKILGISFNLPEGEEFTFHDLNSRANVQCSK
                     AVQQNVGRWFAYFVKHAPRVPFIIIGKDTHGHLVYLKTGPNPHYNSNPSKGGVR"
     gene            3274..5067
                     /locus_tag="PPY92_001775"
     CDS             3274..5067
                     /locus_tag="PPY92_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002469147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HDJ2625767.1"
                     /translation="MTQTNPSFNPSPRYKSKKGWYKDKPPKEKGGMPTEVEIAGPIVI
                     ENKFIDPKTNTEKVIITDEDQKEIVESSDILTTQKLPSLMKYGFSINEKYTKDLGYAL
                     QQMRNQLPISYLYEGVGILETPFGPIVSLSEIYTTKEFDNKSPSDAICDNAYDLTPKG
                     TFDNWFNMYLKEVKGSLLLELAVVFGISALVTSFLKHKHETEFAGIIFSFTGQSSTGK
                     STAASLAVSVAGNPTKGNETLFRNWNATRNALEGYLSNNFGIPIVFDELSSATFRDTT
                     GLLYSITEGQGRQRSNVHGEVKTPKNWGTSVISTSEHSIFNDSAQNDGLRVRTIEINE
                     QFTTNATNADNIKKAVALNYGHVLPLVAKYLINREDEVIQWFYKEVDWFEAKLKDDKS
                     NTGNRMFKRYAVITTSAKILGRVLATDIDIAKIRDYFIDYHGHTISERSLADKAIDVI
                     IQFVAQNRGKFSDEGALKNMFENYGLISLKDDHIEVKMIANVFKQMLNNHQFQDVNNV
                     VNALRDKGFILADRGRQTTKRSVKDNSGKKQSLVFYHLKLDVEFASILGLTKDKSLIQ
                     NWTPANDNKAAKELFKSANEGIGPSGVHEDF"
     gene            5301..6650
                     /locus_tag="PPY92_001776"
     CDS             5301..6650
                     /locus_tag="PPY92_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDJ2625768.1"
                     /translation="MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQ
                     SGRKDNRSGYRQMTQLIQQGQCDILCCYRLNRLHRNLKNALKLIKLCQTYHVHILSVH
                     DGYFDMDQAFDRFKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
                     NGAFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYC
                     GRVNNQYGQFDNMFPSIVSTSIYEQAQRLRSQKQIKQTSSDNQLKQKIKCPCCNTTLT
                     NMTVRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEVCKDFYQNQRIYTKI
                     KGAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSING
                     HQIQHTIQNIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA"
     gene            6672..8300
                     /locus_tag="PPY92_001777"
     CDS             6672..8300
                     /locus_tag="PPY92_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDJ2625769.1"
                     /translation="MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFE
                     VKDIYADRGISGKSMNRPELQRILKDAKEGYIDCVMVYKTNRLARNTSDLLKIVEDLH
                     KQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
                     LPLGYDKIPNSKHELMINQHEANIVKYIFESYAKGHGYRKIANALNHKGYVTKKGKPF
                     SISSITYILANPFYIGKIQFAKYKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQ
                     MRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKDGTKKRIRYYSCSNF
                     RNKGSKVCSANSVRADVIEDYVMKQILEIVKSDKVIQRVVTHVNQENQVDGAALHHDI
                     AYKQQQYDEVQIKLNNLIKTIEDNPDLTSVIRPSIQKYEKQLNDITNQINQLKNQQNE
                     DKPLFDAKEISKLLQHIFHDIKHIEKSRLKALYLSVIDRIDIKKDGNHKKQFYVTLKL
                     NNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI"
BASE COUNT         3285 a         1388 c         1343 g         2523 t
ORIGIN      
        1 atatggaaaa ccatcgtttt cgataaattg ttttaatatc gactagataa atattttact
       61 accttcaact ttagttatat attcgacaaa aggtaaaaat tcagttaaat acttatgatt
      121 aatatctacc gatttaataa ctgtcctgct tctcgttttt ctaatatctt tatccactta
      181 gtaagcaaat atcattatag cttctttccc tctatatcca attattattg atactttaaa
      241 ttaaaaaacc gtatcatata gtagactgac cccaattagt gggaattata taaaaacact
      301 ttcgttgaat tcatattaaa atgaatcata cggaggtgtt ttttctatga aaagagtttc
      361 ttattcagta gaaacaaagt ataaagcagt tgaaatgaaa gcagcaggat tttcaacaaa
      421 agaaattatg aaagaattaa atattaaaaa tagaacacaa gtggaaactt ggtggcgatg
      481 gtatcgaaat ggggaaagtt atagattttc acaacacgtt ggtaaacaat atacctacgg
      541 taaaggatta gaagagctgt cagaagtaga acaattaaaa ttagaaaata agagaaaaga
      601 tatagaattg gatattttaa aaaagtacaa ggcattggaa aggaagtggt accaacagta
      661 gtcatagatt tagtggatca attaaaagga aactattcaa tcaaattgat actagaagta
      721 ttaaatatac ctaaatcaac atattaccga tggaaaaaca aaacctataa aaatgatact
      781 gtaacacaaa aagttattga attatgtgaa gctaaccatt atacctacgg ttatcgtaag
      841 attacagcac tgattaatca atgttataca tcaccaatta atcataagag agtacagaga
      901 atgatgcaga agcatcattt gaactgccga gttagaccta aaaagacgac aaggataggt
      961 aaaccgtatt ataaaacaga caatttatta caaagataat ttaaagcgaa ttgtcccata
     1021 gaagtattaa caaccgatat tacttattta ccatttgatc attctatgtt gtatttatct
     1081 tcgataatgg atatttataa cggagaaatt gtggcgtata aaatagatga taaacaagac
     1141 caacgtttag ttaatgatac attaaatcaa atcgatatac ctgaggggta tatattacat
     1201 agtgatcaag gcagcgttta tacatcttat gcttattatc aattatgcga agaaaaaggc
     1261 attatcagaa gtatgtcccg aaagggaaca cctgccgata acgccccgat agaaagtttc
     1321 cattcctcgc taaagtctga aactctttac atcaataatc agcttaatag ctctaatcat
     1381 attgtaatag atattgtcga aaagtacatt aaaaactata ataataatca aattcaacaa
     1441 aaactaggct acttatcccc tgtaaaatac agagaattaa tagcctagaa catggtgttt
     1501 ttattaagtt cccgttttag gggttcagtg cctatcgatg ataggtgttc ttttttataa
     1561 ttttgaaaaa ataaaaattg agtactgttt tatatactag taaaactaga ctagtacact
     1621 aaccatagca gccaactatg gattaaatat aaaatttgaa tcattctttt tattcttcat
     1681 ggtattaggt ttataagata taatatatag tgttattaaa aataaaggta gtgacactat
     1741 gaataataaa gacatatata gcgatgattt tcaaaatgct ttagtagaca taattttgaa
     1801 cattgataaa ataaatgtca aagaagtttt tgatgattca tgtagggctg tagatcaaga
     1861 taataaaaag gatactttaa atcgttctcc taaagatttt tccataaata taagtgatat
     1921 agtaagatat agtatacttt gcatggaaaa tttaagtagt gacaagattg aagaaaatat
     1981 caatatgatt aaaaatcata gttcagaatt aaatgacaac gacatgttta ttcaagaaaa
     2041 aaataatttt acaactaatt ttaataaatt ctgcaacgct ctaggaataa acatagcaaa
     2101 ctttaaatca ggaaaatcat atgtatttaa accaaaaagt ttatttgtat tatatgatat
     2161 actctcaaac aactcagttt atctacactt ttttaaaaac ggtatcaatg acaaagatat
     2221 agattcttct aatcgtatca aagagaactt atattatttc atcaatcatg aaattactga
     2281 tcaaagagat tatgaacatg ctctgactaa ttattatctc tattttctag tagacagtaa
     2341 tatagaaatt ggaatcaaat ctatcattat taagattttc actgaaaaag gtatcacttc
     2401 taaagataaa cttcagattc ttgaatatta tttagatgaa atagacgatt taaacactga
     2461 aataagtacc aaaattgctg atgctcatgt taaaaatagt acaaactttt tagaaaatga
     2521 aaaagtaagt ataaacagta atttagcaaa atatattact catcgagaca ccagagcttc
     2581 ggcacctaaa aatatgctgt tatcattaat tttaaatagt gacctagtgt ctccaaaaga
     2641 cttagaagaa aaagaactca aatatccaaa gaaaataaca aaagaacatc atgataaatt
     2701 aataaaagaa atcaataaag cactagatta ttataatgat aataacacca ctccttctga
     2761 ctacttttat ataacttcag attaattttt atttaaagcg ctgttaaatc agcgtttttt
     2821 attttgtcaa caaaaatcca tcttaattta gtacggaatt tctaacatat ttttacaagc
     2881 tttcaaatga taacttcttg gctaaaatga acttaagaaa gttaattgtg cgcatattta
     2941 attttcctat cttagttcat tagtaggagg aagttaaatg tatttcaatc aatcagataa
     3001 attcgagcag ttaaaaaaga aaatattagg tatttcattc aatttacctg aaggagaaga
     3061 atttacattt catgacttga acagtcgtgc aaatgtacaa tgttcaaaag ctgtacaaca
     3121 aaatgttgga cgttggttcg catattttgt aaaacatgcg ccacgtgttc catttattat
     3181 tatcggtaag gatactcacg gtcacttagt ttatcttaaa acaggtccta atccacacta
     3241 taacagcaac ccttcgaaag gaggtgttcg ctaatgactc aaactaaccc ttcatttaat
     3301 ccttcaccac gatacaaatc aaaaaaaggt tggtataaag acaagcctcc caaagaaaaa
     3361 ggaggaatgc ctactgaagt tgaaatcgca ggtcctatcg ttatcgaaaa taagtttatt
     3421 gaccctaaaa ctaacactga aaaagtaatt attacagacg aggatcaaaa agaaattgtt
     3481 gaatctagtg atattttaac aacacaaaaa ctgccatctt taatgaagta tggatttagt
     3541 attaatgaaa aatatacaaa agatttaggt tatgcgttgc aacaaatgcg caatcaatta
     3601 cccatttcct acttatatga aggggtaggt atactggaaa caccgtttgg tcctattgta
     3661 tcgttaagtg aaatctatac tactaaagaa tttgataata aatctccatc tgacgccatt
     3721 tgtgataatg cttatgactt aactcctaaa ggtacatttg ataattggtt taacatgtat
     3781 ctaaaagagg ttaaaggttc tcttctttta gaattagcag tcgtatttgg tatttctgcc
     3841 ttagtgacga gtttcctgaa gcacaaacat gaaaccgaat ttgccggaat catttttagt
     3901 tttacaggtc aatcctcaac cggtaagtca actgcagctt ctttagctgt atcagtcgct
     3961 ggtaacccta ccaaaggtaa tgaaactctc tttagaaatt ggaatgccac acgtaatgca
     4021 cttgaaggtt acttgagtaa caattttggt atacctattg tatttgatga actctcatca
     4081 gcaactttta gagatacaac ggggttattg tactccatca ctgaaggtca aggtcgtcaa
     4141 cgttcaaacg ttcatggaga agttaaaacg cctaagaatt ggggtacttc tgtcatttca
     4201 acgtcagaac acagtatttt taatgattca gctcaaaacg atggattacg tgtaagaacc
     4261 atcgagatta atgaacaatt cactacaaat gcgaccaatg ctgacaatat taaaaaagct
     4321 gtagcactaa actatggtca tgttttaccg ttagtagcta aatatcttat aaatcgtgaa
     4381 gatgaagtta ttcaatggtt ttacaaggaa gtagattggt ttgaagctaa attgaaagat
     4441 gataaaagca acacaggcaa tcgtatgttt aagcgttatg cagtcattac aacatctgca
     4501 aaaatattag gtcgtgtttt agctacagat attgatatcg ctaaaataag agactacttt
     4561 attgattatc atggtcatac aatctctgaa cgctcattag ctgataaagc tatcgatgtc
     4621 atcattcaat ttgtagcaca aaacagaggt aagttttcag atgaaggtgc tttgaaaaat
     4681 atgtttgaga actatggatt aatctcatta aaagatgacc atatcgaagt aaaaatgatt
     4741 gcaaacgttt ttaaacagat gctaaacaat catcaatttc aagatgttaa taatgttgtc
     4801 aacgccttaa gagataaagg tttcatatta gctgaccgtg gacgccaaac aactaaaaga
     4861 agtgtcaaag ataatagcgg taaaaaacaa tcacttgtat tttaccatct aaaattagat
     4921 gtagagtttg catctatcct gggattaact aaagataagt cgttaattca aaattggaca
     4981 ccggcaaatg ataacaaagc tgcgaaagaa ctttttaaat ccgctaatga ggggataggc
     5041 ccttcaggag ttcacgaaga tttctgattg tctttatcat acaaccgtga agcatcaaac
     5101 tgaataatcg acgtattgaa gatagaacaa ataacggagt gaaaaacatg gatgaactta
     5161 tcgagatatt agccgatttg gtaattgaag aaatcaatca aatgaatgac taatatgaac
     5221 gcccaacatc ttattgatgt tgggcttata tatcaattca tccataaata tatattatta
     5281 ctgtgaaagg aggagccgat atgaaacaag tcataggcta tttacgtcaa agtacgatga
     5341 aacaacaatc tcttgcagca cagaaacagg ctatcgaagc aatagccgaa aaacatcata
     5401 ttcaacatat caacttttat agcgacaaac aatcaggacg caaagataat cgtagtgggt
     5461 atcgacaaat gacgcaatta attcaacaag ggcaatgtga catattatgt tgttatcgtc
     5521 ttaatagatt acatcgtaat ctgaaaaatg cattaaaact catcaaatta tgtcaaacat
     5581 accatgttca tatcttaagc gtacacgatg gttactttga tatggatcaa gctttcgacc
     5641 gattcaagct taatattttt atcagcttgg ccgaacttga atcagataac attggagaac
     5701 aagtcagaaa tgggcttcaa gaaaaagcaa agcaaggtcg attgattaca acccatgcgc
     5761 cctttggtta cgaatatcac aacggagcat tcatcatcaa tcaaaatgag tcaccaacgg
     5821 taaaggctgt attcaattat tacattaaag gtcatggtta taagaaaatt gcacagttat
     5881 tagaagaaga taacacgtat atcaatcgac aaccctatca agttcgtaac attatcatca
     5941 atcctaatta ttgtggtcgt gtcaacaatc aatatggcca attcgacaat atgtttcctt
     6001 ctattgtttc cacaagtata tatgagcaag cgcagagact tcgatcgcaa aaacaaatca
     6061 aacagacatc ttcggataac caactaaaac aaaaaatcaa atgcccatgt tgtaatacaa
     6121 cacttacgaa tatgaccgtt agaaaaaaga atcatacatt acgttactac gtctgtccta
     6181 aaaacatgaa tgcttctcgc tttgtctgtg attttaaagg catcaatgca caaacacttg
     6241 aagataaagt attagaagtg tgcaaagact tttatcaaaa tcaacgcatc tacacaaaaa
     6301 ttaaaggtgc gattgacaaa cgcatcaaaa gacaaagaaa catagaaaaa catcacacat
     6361 tgactcaaga acaactgata gaaaagttgg cacaaggcat catcgatgca gaaacgttca
     6421 gagaacaaac gcaatcatta cgtcaacaac cgcaacgcac tacatctatc aatggacatc
     6481 aaatacaaca caccattcaa aatattattc aaaaacgttt cacgttaaac atattgtacc
     6541 cctatattga aaccattcac attacgaaag ataaaaatct tataggaatc tatttcaaaa
     6601 atgaaccact caatatcgtc aatcaaacca tgcaatcatc gattgcataa acgaagaaag
     6661 gatgaaacat aatgcaacaa cttaaaacaa aacgtgtcgg tatctatgta cgtgtatcaa
     6721 cagaaatgca aagcacagaa ggttatagta tcgacggaca aatcaatcaa atcaaagaat
     6781 actgtgactt ccatcatttt gaagttaaag atatatacgc tgaccgtggt atttcaggta
     6841 aatctatgaa tcgacctgag ctccaacgta tattgaagga tgcgaaagaa ggctatatcg
     6901 actgtgttat ggtctacaaa acaaaccgat tagctcgtaa tacatctgat cttctcaaaa
     6961 ttgtcgaaga tttacacaaa caaaatgtcg aatttttcag tttatcagag cgtatggaag
     7021 tcaatacttc atcgggtaag ctcatgttac aaatacttgc gagtttctca gaattcgaac
     7081 gtaataacat tgtcgagaat gtatttatgg gtcaaacgag acgtgcccaa gaaggctatt
     7141 atcaaggcaa tttgccgctg ggctatgaca aaatacctaa tagtaaacat gaactgatga
     7201 ttaatcaaca tgaagctaat attgtgaaat atatattcga gtcctatgcc aaaggccatg
     7261 gctatcgtaa aatagccaat gcattaaatc acaaaggcta tgtcactaaa aagggtaaac
     7321 cttttagtat tagttctatc acatatatat tagctaaccc attctatatc ggcaaaattc
     7381 aatttgcgaa atacaaagat tggagtgaaa aacgtcgtaa agggcttaat gataaaccag
     7441 tgatagctga aggtaagcat tcccccatta ttaatcaaga tttatgggat aaagtacaaa
     7501 tgcgtaaaaa acaagtcagt caaaaacccc aagtccatgg caaaggaacg aatctgctta
     7561 caggcattat tcactgtccc caatgtggcg cacctatggc agcaagcaat accacgaata
     7621 cacttaaaga cgggactaag aaacgtattc gttactattc atgtagtaat tttcggaaca
     7681 agggttccaa agtatgttcg gcaaacagtg taagagctga tgtgattgaa gattatgtga
     7741 tgaagcaaat acttgaaata gtcaaaagtg ataaagtcat tcaacgcgta gtaacacacg
     7801 tcaatcaaga aaatcaagtt gatggcgctg cacttcatca cgatattgct tataagcaac
     7861 aacaatatga tgaagtacaa atcaaactaa ataacttgat taaaaccatc gaggataatc
     7921 cggacttaac atcagtaatc agaccaagta ttcaaaaata tgaaaagcaa ctcaatgaca
     7981 ttacgaatca aatcaaccaa ctcaaaaatc aacaaaatga agataagcct ttatttgatg
     8041 ccaaagaaat cagtaaacta ttacaacaca tctttcatga tattaagcac atagaaaaat
     8101 ctcgactcaa agcattgtat ctatcagtga ttgatcgcat tgatattaaa aaagatggta
     8161 atcataaaaa acaattctat gtcacactca aacttaataa cgaaatcata aaacaacttt
     8221 tcaataataa acaactcgac gaagtgcatc tcagcacttc gtctttgttt ttgccccaaa
     8281 cactatatct tactatttag ctaatagaat cattgttata acaataattt tcattatatt
     8341 ctttcattta ttttatctaa ccatttttaa aattctcttt atcaatttca ataacactta
     8401 tctaatgaaa cagtcattgt cgtaattttt ttcataacta cacgatgctt atattgaaat
     8461 tgctaaagaa catcccacaa tgatttttta tcgggaaatg attgggattt gcagattcga
     8521 agaagcagaa ggattgtgc
//
LOCUS       DANBWH010000114         8476 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.117, whole genome shotgun sequence.
ACCESSION   DANBWH010000114 DANBWH010000000
VERSION     DANBWH010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8476
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.117"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            25..1554
                     /gene="aur"
                     /locus_tag="PPY92_001778"
     CDS             25..1554
                     /gene="aur"
                     /locus_tag="PPY92_001778"
                     /EC_number="3.4.24.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloproteinase aureolysin"
                     /protein_id="HDJ2625770.1"
                     /translation="MRKFSRYAFTSMAAVTLLSTLSPAALAIDSKNKPANSDIKFEVT
                     QKSDAVKALKELPKSENVKNIYQDYAVTDVKTDKKGFTHYTLQPSVDGVHAPDKEVKV
                     HADKSGKVVLINGDTDAKKVKPTNKVTLSKDDAADKAFKAVKIDKHKAKNLKDKVIKE
                     NKVEIDGDSNKYVYNVELITVTPEISHWKVKIDAQTGEILEKMNLVKEAAETGKGKGV
                     LGDTKDININSIDGGFSLEDLTHQGKLSAFSFNDQTGQATLITNEDENFVKDEQRAGV
                     DANYYAKQTYDYYKDTFGRESYDNQGSPIVSLTHVNNYGGQDNRNNAAWIGDKMIYGD
                     GDGRTFTSLSGANDVVAHELTHGVTQETANLEYKDQSGALNESFSDVFGYFVDDEDFL
                     MGEDVYTPGKEGDALRSMSNPEQFGQPAHMKDYVFTEKDNGGVHTNSGIPNKAAYNVI
                     QAIGKSKSEQIYYRALTEYLTSNSNFKDCKDALYQAAKGLYDEQTAEQVYEAWNEVGV
                     E"
     gene            1921..2370
                     /locus_tag="PPY92_001779"
     CDS             1921..2370
                     /locus_tag="PPY92_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDJ2625771.1"
                     /translation="MKKSKRLEIVSTIVKKHKIYKKEQIISYIEEYFGVRYSATTIAK
                     DLKELNIYRVPIDCETWIYKAINNQTEQEMREKFRHYCEHEVLSSIINGSYIIVKTSP
                     GFAQGINYFIDQLNIEEILGTVSGNDTTLILTASNDMAEYVYAKLFK"
     gene            2373..2540
                     /locus_tag="PPY92_001780"
                     /pseudo
     CDS             2373..2540
                     /locus_tag="PPY92_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501420.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2717..3952
                     /gene="arcA"
                     /locus_tag="PPY92_001781"
     CDS             2717..3952
                     /gene="arcA"
                     /locus_tag="PPY92_001781"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDJ2625772.1"
                     /translation="MTDGPIKVNSEIGALKTVLLKRPGKELENLVPDYLDGLLFDDIP
                     YLEVAQKEHDHFAQVLREEGVEVLYLEKLAAESIENPQVRSEFIDDVLAESKKTILGH
                     EEEIKTLFATLSNQELVDKIMSGVRKEEINPKCTHLVEYMDDKYPFYLDPMPNLYFTR
                     DPQASIGHGITINRMFWRARRRESIFIQYIVKHHPRFKDANIPIWLDRDCPFNIEGGD
                     ELVLSKDVLAIGVSERTSAQAIEKLARRIFENPQATFKKVVAIEIPTSRTFMHLDTVF
                     TMIDYDKFTMHSAILKAEGNMNIFIIEYDDVNKDIAIKQSSHLKDTLEDVLGIDDIQF
                     IPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNDLLRQKGIKVIEISGSEL
                     VRGRGGPRCMSQPLFREDI"
     gene            3985..4995
                     /gene="argF"
                     /locus_tag="PPY92_001782"
     CDS             3985..4995
                     /gene="argF"
                     /locus_tag="PPY92_001782"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDJ2625773.1"
                     /translation="MTEIQKPYDLKGRSLLKESDFTKAEFEGLIDFAITLKEYKKNGI
                     KHHYLSGKNIALLFEKNSTRTRAAFTVASIDLGAHPEFLGKNDIQLGKKESVEDTAKV
                     LGRMFDGIEFRGFSQQAVEDLAKFSGVPVWNGLTDDWHPTQMLADFMTIKENFGYLEG
                     INLTYVGDGRNNIAHSLMVAGAMLGVNVRICTPKSLNPKEAYVDIAKEKASQYGGSIM
                     ITDNIAEAVENTDAIYTDVWVSMGEESEFEQRINLLKDYQVNQQMFDLTGKDSTIFLH
                     CLPAFHDTNTLYGQEIYEKYGLAEMEVTDQIFRSEHSKVFDQAENRMHTIKAVMAATL
                     GS"
     gene            5086..6516
                     /gene="arcD"
                     /locus_tag="PPY92_001783"
     CDS             5086..6516
                     /gene="arcD"
                     /locus_tag="PPY92_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HDJ2625774.1"
                     /translation="MNESGDNKLSKSSLIGLVIGSMIGGGAFNIMSDMGGKAGGLAII
                     IGWIITAIGMISLAFVFQNLTNERPELDGGIYSYAQAGFGDFVGFISAWGYWFSAFLG
                     NVAYATLLMSAVGNFFPIFKGGNTLPSIIVASLLLWGVHFLILKGVETAAFINSIVTV
                     AKLIPILLVIICMIIAFNFDTFKTGFFGMTSEGVLPFSWASTMSQVKSTMLVTVWVFI
                     GIEDAVIFSSRAKNKKDVGSATVIGLISVLIIYFLLTVLAQGVILQNHISQLDSPSMA
                     QVLATIVGGWGSTLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNG
                     APVNALLITNILVQLFLISMLFTQSAYQFAFSLASSAILYPYMFSAFYQVKYTLEHRQ
                     QATTKQWTIGIIASIYAIWLIYAAGINYLLLTMLLYIPALLVYSIVQKNNQTRLIKSD
                     YILFMIIIVLAVIGLIKLLMGTINVF"
     gene            6533..7474
                     /gene="arcC"
                     /locus_tag="PPY92_001784"
     CDS             6533..7474
                     /gene="arcC"
                     /locus_tag="PPY92_001784"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDJ2625775.1"
                     /translation="MKEKIVIALGGNAIQTKEATAEAQQTAIRRAMQNLKPLFDSPAR
                     IVISHGNGPQIGSLLIQQAKSNSDTTPAMPLDTCGAMSQGMIGYWLETEINRILTEMN
                     SDRTVGTIVTRVEVDKDDPRFNNPTKPIGPFYTKEEVEELQKEQPDSVFKEDAGRGYR
                     KVVASPLPQSILEHQLIRTLADGKNIVIACGGGGIPVIKKENTYEGVEAVIDKDFASE
                     KLATLIEADTLMILTNVENVFINFNEPNQQQIDDIDVATLKKYAAQGKFAEGSMLPKI
                     EAAIRFVESGENKKVIITNLEQAYEALIGNKGTHIHM"
     gene            7573..8277
                     /locus_tag="PPY92_001785"
     CDS             7573..8277
                     /locus_tag="PPY92_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDJ2625776.1"
                     /translation="MTENFILGRNNKLEHELKALADYINIPYSILQPYQSECFVRHYT
                     KGQVIYFSPQESSNIYFLIEGNIIREHYNQNGDVYRYFNKEQVLFPISNLFHPKEVNE
                     LCTALTDCTVLGLPRELMAFLCKANDDIFLTLFALINDNEQQHMNYNMALTSKFAKDR
                     IIKLLCHLCQTVGYDQDEFYEIKQFLTIQLMSDMAGISRETAGHIIHELKDEKLVVKD
                     HKNWLVSKHLFNDVCV"
BASE COUNT         3099 a         1175 c         1646 g         2556 t
ORIGIN      
        1 ttaagatttc aggaggaatg aaatgtgagg aaattttcaa gatatgcatt tacaagtatg
       61 gcagcagtaa cattgttgag cactttatca ccagcagcat tagcgattga ttcaaaaaat
      121 aaaccagcta attctgatat taaatttgag gtgactcaaa agagtgatgc ggtcaaagca
      181 ttaaaagaat tgcctaaatc cgaaaatgta aaaaatattt atcaagatta cgctgttact
      241 gatgtaaaaa ctgataaaaa aggatttacg cattatacat tgcaaccgag tgttgatggt
      301 gttcatgcac ctgacaaaga agtgaaagta cacgcagaca aatcaggaaa agtcgtttta
      361 atcaatgggg atactgatgc gaagaaagta aagccaacga ataaagtgac attaagtaaa
      421 gatgacgcag ccgacaaagc atttaaagca gttaagattg ataagcataa agcgaaaaat
      481 cttaaagata aagtcattaa agaaaacaaa gttgaaatcg atggtgacag taataaatac
      541 gtttataatg ttgagttaat tacagtgaca ccagaaattt cacattggaa agttaaaatt
      601 gatgctcaaa ctggcgaaat tttagaaaaa atgaacttag ttaaagaagc tgcagaaact
      661 ggtaaaggaa aaggtgtact tggcgataca aaagatatca atatcaatag tattgacggt
      721 ggatttagcc tagaagattt aacgcatcaa ggtaaattat cagcatttag ctttaatgat
      781 caaacaggtc aagcaacatt gattactaat gaagatgaaa acttcgtaaa agatgagcaa
      841 cgtgctggcg tagatgcaaa ttattacgct aaacaaacat atgattatta caaagacaca
      901 tttggtcgtg aatcatatga caaccaaggt agtccaattg tttcattaac gcatgttaat
      961 aactacggtg gtcaagataa cagaaataat gccgcatgga ttggtgacaa aatgatctat
     1021 ggtgatggtg atggtcgcac attcacaagt ttatcgggtg caaatgacgt agtggcacac
     1081 gaattaacac acggtgtgac acaagagact gcgaacttag aatataagga ccagtcaggc
     1141 gctctaaatg aaagcttttc agatgttttt ggatactttg tagatgacga ggatttctta
     1201 atgggtgaag atgtctacac acctggaaaa gagggggacg ctttacgcag catgtcaaac
     1261 ccagaacaat ttggtcaacc agctcatatg aaagactatg tattcactga aaaagataat
     1321 ggtggcgtac atacgaattc tggaattcca aataaagcag cttataacgt gattcaagca
     1381 atagggaaat ctaaatcaga acaaatttac taccgagcat taacggaata cttaacaagt
     1441 aattcaaact tcaaagattg taaagatgca ttataccaag cggctaaagg tttatatgac
     1501 gagcaaacag ctgaacaggt gtatgaagca tggaatgaag taggcgtgga gtaaaaatat
     1561 ataaacaaga agaagtaatg ttaaacactt ataaataatt aaattttaaa tacagcttat
     1621 atatggggta tacctacatt gataattgac tttgtaggta taccctctct ttgtaattag
     1681 atgagaggag tgtgagggtt gtctgccgaa agactactcg gcagtctaaa atcattacaa
     1741 gtagtagata tgtgataatt aaatgctgac ttagaataca aaattcattt taaaagttgt
     1801 cacaaaaaat ttacatgtat ttttattatc ttttgcaaaa caaagtgtta aattataaat
     1861 gaaacatgca tgaatttatt ttttaataca agaaacgtaa ctaccaaagg agtttacaat
     1921 atgaagaaaa gtaaacgatt agaaattgtt tctacaatag ttaaaaagca taagatttat
     1981 aaaaaagaac aaatcatttc atatattgaa gaatattttg gtgtaagata tagtgcaaca
     2041 acaattgcta aagacttgaa agaacttaat atatatcgtg tccctatcga ttgtgaaaca
     2101 tggatttata aagctattaa taatcaaaca gaacaagaga tgagagaaaa gtttagacac
     2161 tattgtgaac atgaagttct aagttcaatc atcaatggtt catacattat cgttaaaacc
     2221 tcacctggtt tcgcccaagg cataaactat tttatcgatc agctaaatat agaagagata
     2281 ttaggtacgg tgagtggaaa tgacactaca ttaatcttaa ctgcctcaaa tgatatggca
     2341 gaatacgtat atgcaaaatt atttaaatag acatgtatca aatgaataat aaaaattagt
     2401 ttcgtatcac gtgtacacaa gttagttacc aaatattaac ttgtgtacgc gtttttttat
     2461 ggaaagaaag aattcatagt cattcatttg actgtataaa aaactttata caacgtgttt
     2521 ttgtgggtct ttttgaataa aaaatgtata ttttgaccca aaacaccttt atttatgtat
     2581 aaaaatccat tattatgtat tgtataacaa aaagatatga aattttcgac tttctttatg
     2641 tgaatataat cacatgtaag cgtttgaaga ttgtctatac tctaaatgaa ttcaaagata
     2701 aaaggaggaa atagacatga cagatggtcc aattaaagta aatagcgaaa ttggagcttt
     2761 aaaaactgtg ttacttaagc gtcctggaaa agaattagaa aatttagtac ctgattattt
     2821 agatggatta ctatttgatg atattccata tttagaagta gctcaaaaag agcatgacca
     2881 ttttgcgcag gtgctaagag aagagggtgt tgaagtactt taccttgaga agttagcagc
     2941 tgaaagtatt gaaaatcctc aagtaagaag tgaatttatt gatgatgtat tagcagagtc
     3001 taaaaaaaca atattaggtc atgaagaaga aattaagaca ttatttgcga cactttctaa
     3061 tcaagaactt gtagataaaa taatgtcagg ggtacgtaag gaagaaatta atccgaaatg
     3121 tacacatcta gtagagtaca tggatgataa gtatccattc tatttagatc caatgccaaa
     3181 cctttatttt actagagatc cacaagcctc aataggacac ggtataacaa tcaatcggat
     3241 gttctggaga gcacgacgac gagaatcaat atttattcaa tatattgtaa agcatcatcc
     3301 tagatttaaa gatgcgaata ttccaatctg gttagatcga gattgcccat tcaatattga
     3361 aggcggcgat gaacttgttt tatctaaaga tgtcttggct ataggcgttt cagaacgtac
     3421 atctgcacaa gctattgaaa agttagcgcg acgtattttt gaaaatccgc aggcgacgtt
     3481 taaaaaagta gtagcaattg aaattccaac tagtcgaact tttatgcact tagatacagt
     3541 atttacaatg atagattatg acaaatttac aatgcattca gccattttaa aggcagaagg
     3601 caatatgaat atatttatta ttgaatatga tgacgtaaat aaagatattg ccatcaaaca
     3661 atctagtcat ttaaaagata ctttagaaga cgtactaggt atagatgata tccaattcat
     3721 tccaacagga aatggtgatg tcattgatgg tgctagagag caatggaatg atggctcaaa
     3781 tacattatgt ataagaccag gcgttgtagt gacttacgat agaaactatg tatcgaatga
     3841 tttattgaga caaaaaggca taaaagtcat tgaaatatct ggtagcgagc tggtacgtgg
     3901 acgtgggggc cctagatgta tgagtcaacc actattcaga gaagacattt aacgtaataa
     3961 attatagaag cgagggtgaa aataatgaca gaaattcaaa aaccgtatga tttaaaaggc
     4021 agatcattat taaaagaaag tgattttacc aaagcagaat tcgaaggact tattgatttt
     4081 gcaattacat taaaagagta taagaaaaac ggtattaagc atcactactt atctggaaaa
     4141 aatattgcac tactattcga aaagaattcg acgagaacgc gtgcagcgtt tacagttgcg
     4201 tctattgatt taggtgcgca tccagaattt ttagggaaaa atgatattca attaggcaaa
     4261 aaagaatctg tagaggatac tgcgaaagta ttaggtagaa tgttcgatgg tattgaattc
     4321 cgtggttttt cacaacaagc tgttgaagat ttagcgaagt tctctggtgt accggtgtgg
     4381 aatggattaa cagacgattg gcatcctaca caaatgttag ctgattttat gacaataaaa
     4441 gagaattttg gatatctaga aggaataaac ttaacttacg ttggagatgg acgtaataat
     4501 attgcgcatt cattaatggt agcaggtgct atgttaggtg ttaatgtaag aatttgtaca
     4561 cctaaatcat taaatccaaa agaggcatat gttgatattg caaaagaaaa agcgagtcaa
     4621 tatggtggtt caatcatgat tacggataat attgcagaag cagttgaaaa tacagatgct
     4681 atatatacag atgtttgggt atcgatgggt gaagaaagtg aatttgaaca acgtattaat
     4741 ttattaaaag actatcaagt gaatcaacag atgtttgatt taacaggtaa agattcaacg
     4801 atattcttac attgtttacc agcattccat gatacaaata cactttatgg acaagaaatt
     4861 tatgaaaaat atggattagc tgaaatggaa gttacagacc aaatctttag aagtgaacat
     4921 tcaaaagtgt ttgatcaagc tgaaaataga atgcatacaa ttaaggcagt aatggcagca
     4981 acattgggga gttaatcact aaatggaacg atatgaatat gatgtgtctg atgatataag
     5041 tgtcatgtac agacacctca tattggtatt aaaggagaaa tgaatatgaa cgaatcagga
     5101 gataacaaac tcagtaaatc ttctttaatt ggactagtta taggatccat gattggtggc
     5161 ggtgcgttca atataatgtc tgatatgggc ggtaaagccg gtggattagc cattattatt
     5221 ggttggatta ttacagctat aggaatgatt tcattagcgt tcgtatttca aaatttaacc
     5281 aatgaacggc cggagctaga cggtggtatt tatagttatg ctcaagcagg atttggcgat
     5341 tttgtaggat ttatcagtgc ttggggatat tggttctcag cgtttttagg caatgttgcc
     5401 tatgcaacac tattgatgtc agcagtaggt aactttttcc cgatttttaa aggaggcaac
     5461 acattaccaa gtattattgt cgcctcgtta ctactctggg gtgtccattt cttgatttta
     5521 aaaggcgttg aaacagcagc atttatcaat agtattgtta ctgttgcaaa gttaatcccg
     5581 attttacttg taatcatatg catgataatt gcattcaatt ttgacacttt taaaacaggc
     5641 tttttcggta tgacgtcaga gggtgtattg ccatttagtt gggcgagcac aatgagccaa
     5701 gttaaaagta cgatgctagt gacagtttgg gtgtttatcg gtatcgaaga tgcagtaatt
     5761 ttttctagta gagctaaaaa taaaaaagat gtaggtagtg ccacggttat aggacttata
     5821 tcagttttaa ttatctattt cttattaact gtattagctc aaggcgtgat tttgcaaaat
     5881 catatttctc aattagattc gccaagtatg gcacaggtgc ttgcaactat tgtaggtggt
     5941 tggggatcta cacttgtaaa tattggttta attatttcgg tactaggtgc atggttagga
     6001 tggacattgc ttgctggtga attacctttc attgttgcaa aagatggatt atttccaaag
     6061 tggtttgcta aagaaaataa aaatggagca cctgtaaatg cactgcttat taccaatata
     6121 ttagtacaat tatttttaat aagtatgcta tttacacaga gtgcgtatca atttgcattt
     6181 tcactagcat caagtgctat tttataccct tacatgttca gtgcatttta ccaagttaaa
     6241 tacactttag agcatcgaca gcaagcaact actaaacaat ggacgattgg catcatagcc
     6301 tcaatttatg ctatatggct tatatatgca gcaggtatca attacttatt attgacgatg
     6361 ttactttata ttccagctct tcttgtttat tcaatcgttc aaaagaataa tcagacacgt
     6421 ttgattaaat cagactatat tctttttatg attattatcg tacttgcagt tatcggatta
     6481 attaagttat tgatgggaac gataaatgtt ttttaaaagg agcgacaaaa atatgaaaga
     6541 gaaaattgtc attgcattag gcggtaatgc gatacagaca aaagaagcaa cagctgaagc
     6601 acaacaaaca gctattagac gtgcgatgca aaaccttaaa cctttatttg attcaccagc
     6661 gcgtattgtc atttcacatg gtaatggccc acaaattgga agtttattaa tccaacaagc
     6721 taaatcgaac agtgacacaa cgccggcaat gccattggat acttgtggtg caatgtcaca
     6781 gggtatgata ggctattggt tggaaactga aatcaatcgc attttaactg aaatgaatag
     6841 tgatagaact gtaggcacaa tcgttacacg tgtggaagta gataaagatg atccacgatt
     6901 caataaccca accaaaccaa ttggtccttt ttatacgaaa gaagaagttg aagaattaca
     6961 aaaagaacag ccagactcag tctttaaaga agatgcagga cgtggttata gaaaagtagt
     7021 tgcgtcacca ctacctcaat ctatactaga acaccagtta attcgaactt tagcagacgg
     7081 taaaaatatt gtcattgcat gcggtggtgg cggtattcca gttataaaaa aagaaaatac
     7141 ctatgaaggt gttgaagcgg ttatagataa agattttgct agtgagaaat tagcaacgct
     7201 gattgaagca gataccttaa tgattcttac gaatgtagaa aatgtattta ttaactttaa
     7261 tgaacctaat caacaacaaa tcgatgatat tgatgtagca acactgaaaa aatacgcggc
     7321 acaaggtaag tttgcggaag gatcgatgtt gccaaaaata gaagctgcga tacgatttgt
     7381 tgaaagtggg gaaaacaaaa aagttatcat taccaattta gagcaggcat acgaagcttt
     7441 gattggtaat aaaggtacac acattcacat gtagcacttt atcacgcgac aaaacattaa
     7501 atatgtttct ccgttgattc aaatgaaaaa gttgtctgct gacactttgc aaggtttgaa
     7561 ggagtttaac ttatgacaga aaactttatt ttgggtagaa ataataaatt agaacatgaa
     7621 ctaaaggcat tagcagatta cattaatatc ccatatagta tattacaacc atatcaaagt
     7681 gaatgttttg tcagacatta tacgaaaggc caagttattt atttttcgcc acaagaaagt
     7741 agcaatattt actttttaat tgaaggtaat attattagag aacattacaa tcaaaatgga
     7801 gatgtatatc gttattttaa taaagagcaa gtattatttc caatcagtaa cttatttcat
     7861 ccgaaagagg ttaacgaatt gtgtacagca ttaaccgatt gtacagttct tggattgcct
     7921 agagaattga tggctttttt gtgcaaagct aatgatgata tatttttgac actttttgca
     7981 ttaataaatg ataatgagca gcaacacatg aactataaca tggcattaac aagtaaattt
     8041 gctaaagatc gaattattaa attgttatgt catctatgtc agacagtagg gtacgatcaa
     8101 gatgaatttt atgaaatcaa acagttttta actattcaac tcatgagtga tatggctggg
     8161 atttcccggg aaacagctgg tcatattatt catgaactta aagatgaaaa acttgttgtt
     8221 aaagatcata aaaattggtt agtaagcaaa catttattca atgatgtatg tgtttaatat
     8281 acaatgtaaa atgaataagt tgaacatgag gtctaacgta catttatacg ttaggccttt
     8341 tttgctagca tgatgaataa tttaaaatgt tagttaaatt tgattgttga aattacagta
     8401 aaatttaagg tgatgaaaaa tttagaactt ctaagttttt gaaaagtaaa aaatttgtaa
     8461 tagtgtaaaa atagta
//
LOCUS       DANBWH010000115         8344 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.118, whole genome shotgun sequence.
ACCESSION   DANBWH010000115 DANBWH010000000
VERSION     DANBWH010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8344
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.118"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(224..1090)
                     /locus_tag="PPY92_001786"
     CDS             complement(224..1090)
                     /locus_tag="PPY92_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDJ2625777.1"
                     /translation="MNEQWLEHLPLKDIKEISPVSGGDVNEAYRVETDTDTFFLLVQR
                     GRKESFYAAEIAGLNEFERAGITAPRVIASGEVNGDVYLVMTYLEEGASGSQRQLGQL
                     VAQLHSQQQEEGKFGFSLPYEGGDISFDNHWQDDWRTIFVDKRLDHLKEELLNRGLWD
                     ANDIKVYDKVRSQIVEELEKHQSKPSLLHGDLWGGNYMFLKDGRPALFDPAPLYGDRE
                     FDIGITTVFGGFTSEFYDAYNKHYPLAKGASYRLEFYRLYLLMVHLLKFGEMYRDSVA
                     HSMDRILQGTTS"
     gene            1308..2372
                     /locus_tag="PPY92_001787"
     CDS             1308..2372
                     /locus_tag="PPY92_001787"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDJ2625778.1"
                     /translation="MYKLIKPFLFKIEPEKAHGLTIDALKTLQKFPVLFPVVDKLFTY
                     KNPTLSQTIQGNTYDNPIGLAAGFDKSCEVPKALEHLGFGALELGGITPKPQPGNPQP
                     RMFRLLEDDALINRMGFNNIGMNKALSHLRKNAYQVPVGINVGVNKMTPYEARYQDYI
                     KVIDTFKHDVSFFTVNISSPNTENLQNFHDKDEFSMLCQALTTFKKQHDVTVPIYLKL
                     TSDMDFDGLKALLPAITETFDGIILANTTRQRDGLTSANKVEEGGLSGRPLFERNLKL
                     IKYAYQQTNGEFLIIGTGGVFSTEDAIKMMRHGASLIQIYSSLVIEGPGLTKKMNKGI
                     ARYLKDHHFDNVSDIIGLDA"
     gene            2527..2802
                     /locus_tag="PPY92_001788"
     CDS             2527..2802
                     /locus_tag="PPY92_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625779.1"
                     /translation="MTKETDNKKTVATSDNKDTQQQNQTEEDERQGLNGYRKTDLDLE
                     IEQELREMMKTGENETKNDYKKFKVFSLISTLVIVILAIIRFVHKMI"
     gene            complement(2988..3710)
                     /locus_tag="PPY92_001789"
     CDS             complement(2988..3710)
                     /locus_tag="PPY92_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDJ2625780.1"
                     /translation="MINAEPIISKMKNQKINYDKVLKKLIGQWEREAIRPKILLHSCC
                     APCSTYTLEFLTQYADIAIYFANSNIHPKNEYLRRAKVQEQFVEDFNRKTGANVKYIE
                     APYEPHKFVKMVKDKELADEKEGGLRCTACFEMRLDIVAKAAVEHGYDYFGSAITLSP
                     KKNAQLINELGMDVQKIYDVNYLPSDFKKSKGYERSIEMCNDYNIFRQCYCGCVFAAM
                     QQGIDFKTVNKEAKAFLEQYPD"
     gene            4064..4513
                     /locus_tag="PPY92_001790"
     CDS             4064..4513
                     /locus_tag="PPY92_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HDJ2625781.1"
                     /translation="MTALFPYIAFENSKEALAYYEEVFGATDVKRLEVGEEQASHFGM
                     TKEEAQEATMHAEFEVLGVKVLCSDSFGRADKINNGISLLIDYDVNNKEDADKVEAFY
                     EKIKDHSSIEIELPFADQFWGGKMGVFTDKYGVRWMLHGQDYTAIQQ"
     gene            complement(4594..5181)
                     /locus_tag="PPY92_001791"
     CDS             complement(4594..5181)
                     /locus_tag="PPY92_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDJ2625782.1"
                     /translation="MTIDRRIRKSQVAMRNAFIELLHQHQLEEITVQQIADLADVNRS
                     TFYTHYYDKYDLLEKLEDQQLEEIRDFIHDEKLNGAVKLSTDNIHQIMTVLIDMIEKD
                     IAFYQLMFRMGKDSNIHEKLYDLIMCHLQRYKNENDQINGIPFSYFMSYVSGAGLSLI
                     RHWVEDPNRIDKVDLIMHFNTIVNQGPATIIKNIQ"
     gene            complement(5159..5267)
                     /locus_tag="PPY92_001792"
                     /pseudo
     CDS             complement(5159..5267)
                     /locus_tag="PPY92_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501367.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5324..7006
                     /locus_tag="PPY92_001793"
     CDS             5324..7006
                     /locus_tag="PPY92_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HDJ2625783.1"
                     /translation="MNQHLLGNPKLTVTHVNEVKTGINHIVVDSVQYGNQEMIMEKDV
                     TVEMRDGEKLYINIFRPNKDGKFPVVMSADTYGKDNKPKITNMGALWPTLGAIPTSSF
                     TPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAAKQPWSN
                     GNIGTNGVSYLAVTQWWVASLNPPHLKAMIPWEGLNDMYREVAFHGGIPDTGFYRFWI
                     QGIFARWTDNPNIEDMIQAQQEHPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNR
                     GSFEGFKQAASEEKWLYVHGRKEWESYYARENLERQKSFFDFYLKEENNDWKDTPHVI
                     YEVRDQFYKGEFKSASAFPLPNTEYTPLYLNAENHTLNHAKISSAYVAQYDSEDKRQD
                     VSFKYTFDKDTELVGNMNLKLWVSTTDSDDMDLFAGIKKLDRRGNEVNFPDFNHIENG
                     QVATGWLRVSHRELDQEKSTIAQPWHKHETELKLSQDEIVPVEIELLPSGTLFKQGET
                     LEVVVKGSEIVIGNSTPGMKTRYEHEETINNGMHMIYTGGKYDSQLIIPIVN"
     gene            complement(7104..7739)
                     /locus_tag="PPY92_001794"
     CDS             complement(7104..7739)
                     /locus_tag="PPY92_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDJ2625784.1"
                     /translation="MKKVISLFVAILSVLIFTDNANAENNKPEGVNENSNLIPVRQPD
                     ANYPGPVSDIARVKKLDNLIDGTKDPNVIKQLKDDNEVPNNAVKAKDSKIVSDCRHLL
                     KHPDNLIDGTKDPNVINQLKNANGTSNNSAVNPSIKANDIHTKSNKQMAKQQSIHLDS
                     KSKSVHKQQLKELPKTGVNNESNTAIMALFIGLILLVVSLVTRFIKLNSHQ"
     gene            complement(7755..>8344)
                     /locus_tag="PPY92_001795"
     CDS             complement(7755..>8344)
                     /locus_tag="PPY92_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HDJ2625785.1"
                     /translation="NAPTKEDIAIIKLNSNLGNKTGYLTLNTHISKGENIEISGFPGD
                     KSDNRQYKGKGKLESFDENEMYYTVDTFSGQSGSAIRDSKNNIIGVHAYGRYNHNSGV
                     RINDLKLDYINYWIGKYRSHPYNKKVKVIKSKYIYWKNIEITKKGDNKLIKKDKAYTA
                     KLYYNIPNGYKYYSLYDEKNRWMGYFNSNDIKVVK"
BASE COUNT         2748 a         1486 c         1279 g         2831 t
ORIGIN      
        1 tcaataaata tgtatcattc ctagaatttt attgaacaaa aagttataaa atcactttta
       61 ttgcaactca accttgtttt gcattacaga aattaacaat agcgattaaa attttatttt
      121 ccagcaataa aaaagcacaa atatttttat tgaggcaact gaaggccttc tcttcataaa
      181 atatctgtgc atattatcta tttatctcaa tctaacgtct taactaactt gttgtacctt
      241 gtaaaatcct atccatagaa tgcgcaacac tatcacggta catctcacca aatttcaata
      301 aatgcaccat caataaatat aaacgataaa attcaagtct ataggatgct ccttttgcga
      361 gcggataatg tttattatac gcatcgtaaa attcgctcgt aaaaccacca aataccgttg
      421 taataccgat atcaaattct ctgtcaccat ataatggtgc tggatcaaat aatgcaggac
      481 gtccatcttt taaaaacata taattaccgc cccataaatc accatgtaat aaagacggtt
      541 tactttgatg cttttctaat tcctccacaa tttgacttcg cactttgtca tatactttga
      601 tatcgttggc atcccatagc cctctattta gcaactcttc tttcaaatga tccaaacgtt
      661 tatcgacaaa aatggtacgc caatcatctt gccaatgatt atcaaaagaa atatcgccac
      721 cttcataagg taatgagaag ccaaatttgc cttcttcttg ttgctgactg tgtaattgag
      781 ctacgagttg acctaattga cgttgactcc ctgaagctcc ttcttctaaa tacgtcatca
      841 ctaaatacac atcaccgtta acttcgccac ttgcaattac tctaggtgcc gtgatacctg
      901 cacgttcaaa ttcatttaaa cctgcaattt ctgcagcata aaatgattct ttacgtccac
      961 gttggacaag taagaaaaat gtatccgtat ctgtttcgac tcgatatgct tcgtttacat
     1021 caccaccact cactggtgaa atctctttaa tatcttttaa aggtaaatgc tctaaccatt
     1081 gttcattcat acaaacacct tccaatctac ttactttata ctataatatt gcctaattag
     1141 gatgatttaa tcttaaaaac gttattcaaa cttgcgtcaa ttttcaaaac tttttagttt
     1201 taactaggaa attatgtagg gaaatgctag tctattaaaa gaatatattt cacatatatt
     1261 aagttgagga tagtaagcct caaatataat gaaataggag catactcatg tacaaattaa
     1321 ttaaaccttt cttattcaaa atcgaacccg aaaaagcaca cggactaact atcgatgcat
     1381 taaaaacgtt acaaaagttt ccggttttat tcccagtcgt cgataaacta tttacttata
     1441 agaatccaac gttatcacaa acgatacaag gtaatacgta cgacaatcca attggcttag
     1501 cagctggttt cgacaaatct tgcgaagtac caaaagcatt ggaacacctt ggattcggtg
     1561 ctttagaatt aggtggtatc acacctaaac ctcaaccggg taaccctcaa ccacgcatgt
     1621 ttagattatt agaagatgac gccttgataa atcgaatggg cttcaataat attggtatga
     1681 acaaagcact cagtcatttg cgtaaaaatg cttatcaagt acctgttggt atcaatgttg
     1741 gtgtgaataa aatgacacct tatgaagcgc gttatcaaga ttatataaaa gtcattgata
     1801 cgtttaaaca cgacgtttca tttttcacag tcaatatcag ttctccaaat actgaaaacc
     1861 ttcaaaattt ccatgataaa gatgaatttt caatgttatg tcaagctttg acaacattta
     1921 aaaaacagca cgatgtaaca gtgccaattt acttaaaact aacgtctgat atggatttcg
     1981 atggcttaaa agcactatta ccagcgatta ctgagacatt tgacggtatc atcttagcaa
     2041 acacaacgcg acaacgagat ggtttaactt ctgctaataa agtcgaagaa ggcggtttga
     2101 gtggtcgtcc attatttgaa cgtaatttaa aattgattaa gtatgcttat cagcaaacaa
     2161 atggtgaatt tttaattata ggtacaggcg gcgtattcag tactgaagat gcaatcaaaa
     2221 tgatgcgtca cggtgcgtca cttattcaaa tttattcatc acttgttatt gaaggcccag
     2281 gtttaactaa gaaaatgaac aaaggcatcg cacgttactt aaaagatcat cattttgaca
     2341 atgtcagtga tattatagga ctagatgcct aatccaacgt agtcaaataa cactttaaaa
     2401 cttgctgaat cgtcttatta aaattatgat ataactcaag aagatatatt tatcaccatt
     2461 tttcaaaata atggcttaaa tatcacatat cattaaatat agcgttttat aacggaggcg
     2521 tttgttatga ctaaagaaac agataataaa aaaacagtag cgacttctga caacaaagac
     2581 acacagcaac aaaatcaaac tgaagaagat gaacgtcaag gtttgaatgg ttatcgtaaa
     2641 acagatcttg atttagaaat tgagcaagag ctacgtgaaa tgatgaaaac aggagaaaat
     2701 gaaacgaaaa acgactacaa aaagtttaaa gtgttttcac ttatttcaac acttgtcatt
     2761 gtcattttag caattataag atttgttcat aaaatgattt aattagagtg agacattgtt
     2821 ttatgtctca ggatccagtt attcagtata gctacaatat ttacgattat ataaataacc
     2881 cgagatttta gtatgattca tttcactaaa atctcgggtt tctatttgat aatttttaat
     2941 gggatatggc atgtatacga gcctgccttt tatctcattt ccaatgatta atctggatat
     3001 tgttctaaaa atgctttcgc ttctttatta actgttttaa aatcaatacc ttgttgcatc
     3061 gctgcaaaga cacatccaca ataacactgc ctaaagatat tatagtcatt acacatttct
     3121 atggaacgct cataaccttt acttttctta aaatcacttg gcaaatagtt cacatcgtat
     3181 attttttgga catccatacc aagttcattg attaattgtg cgttcttttt aggtgataac
     3241 gtgattgcac tgccaaaata atcatagcca tgttctacag ctgctttcgc tacaatatcc
     3301 aaacgcattt caaaacaagc ggtacaacgt aagccgcctt ctttttcatc agctaattct
     3361 ttatccttca ccattttcac aaacttatgc ggttcataag gtgcttcaat atacttcaca
     3421 ttcgcaccag tcttgcgatt aaaatcttcc acaaattgtt cttgcacttt agcacgtcgt
     3481 aaatattcat ttttcggatg aatatttgaa ttcgcgaaat aaattgcaat gtctgcatat
     3541 tgtgttaaaa actctaatgt atatgtacta caaggtgcac aacaactatg caataagatt
     3601 ttaggtctga ttgcttctct ttcccactgc ccgattaatt tcttcaacac cttgtcataa
     3661 ttaatttttt gatttttcat tttactgata ataggttcag cattaatcat gatttaacct
     3721 cccacattta atcattaact tctattatat atgattcata ttaaatgtca gtcaaaaaag
     3781 ttagaaattc attttaatgc atttatattt cgaaagtccc ttatgtatct aaaagcattt
     3841 tttaaacttg aattttaaaa cactaaacaa cacatacgtc tctgtgtcat tttcattttt
     3901 tgtatgtcat atatatgttt acttcattta aatcaatttc atcttacaat ttatcgtgta
     3961 ttttacaaaa gattgacttc aattcatcgt aaaagttata cttttgccat tttttaatgt
     4021 aacatggtgt tagtaataaa aatactacat tgaggtgttt tacatgacag cattattccc
     4081 ttatatcgct tttgaaaatt caaaagaagc ccttgcatat tacgaagaag tatttggtgc
     4141 aactgacgtt aaacgtttag aagttggcga agaacaagcg tcacattttg gtatgactaa
     4201 ggaagaagcg caagaagcaa ctatgcatgc tgaatttgaa gtgcttggcg taaaagtgct
     4261 atgttctgat tcttttggtc gcgctgacaa aattaataat ggcatatcat tattaattga
     4321 ttatgatgtt aacaataagg aagatgctga taaagttgaa gcattctatg agaaaattaa
     4381 agatcattct tcaattgaaa tagaattacc gtttgctgac caattctggg gtggcaaaat
     4441 gggcgtcttt accgataaat acggtgttcg ttggatgtta catggtcaag actatactgc
     4501 aatccaacaa taatgttatt gataaagata tcatttaata agcttatgtt atttagcaag
     4561 gcaagccact ctcgattcat ggcttgcctt attttattgt atatttttaa ttatcgttgc
     4621 tggcccttga ttgacaatcg tattaaaatg cattattaag tcaactttgt ctatacggtt
     4681 tggatcttct acccaatgtc ttatcaaaga caatcccgca cctgaaacat aactcatgaa
     4741 ataagaaaat ggtataccat taatttgatc attttcattt ttataacgtt gtaaatgaca
     4801 cataattaag tcatatagtt tttcgtgtat atttgaatct ttacccatac gaaacatcaa
     4861 ttgataaaat gcgatgtctt tttctatcat atctattaaa acagtcataa tttgatgtat
     4921 gttatccgtg gataacttaa ctgctccatt taacttctca tcatgaatga agtctcttat
     4981 ttcctccaac tgctggtcct ctaatttttc aagcaaatca tatttatcat aataatgcgt
     5041 ataaaatgta ctacggttaa catcagctaa atctgcaatt tgttgcacag taatctcttc
     5101 taattggtgt tgatgtaaaa gttcaataaa tgcatttctc attgcaactt gtgattttct
     5161 aatacgtcga tctatagtca tttatatcaa gtcctcccca atgattataa acaattataa
     5221 acgttatgtt cattatccca caaatctcca acattgatga ttggcacaca atgtttacct
     5281 gtttaatata ggtgatacaa acaaacagaa aaaggtgata acaatgaacc aacatttact
     5341 aggaaatcca aaattaactg taactcatgt caatgaagtg aaaaccggta ttaaccacat
     5401 cgttgtcgac agtgttcaat atggaaatca agaaatgatt atggaaaaag atgtcactgt
     5461 ggaaatgcgc gatggcgaaa aattatatat taatattttc agaccaaata aagatggcaa
     5521 attccctgta gttatgtctg cagatactta cggtaaagat aataagccta aaatcaccaa
     5581 tatgggtgcc ctttggccaa cattaggcgc cattccgaca tctagtttta cacctgaaga
     5641 atcaccagac ccaggatttt gggtaccaaa cgattatgtt gtagttaaag ttgcattacg
     5701 cggtagtgac aaatccaaag gcgtcttatc tccatggtca aaaagagaag cggaagatta
     5761 ttacgaagtg attgaatggg cagcaaaaca gccatggagt aatggaaata tcggaacaaa
     5821 tggtgtttct tatcttgcgg tgactcaatg gtgggtcgca tcattaaatc caccacattt
     5881 aaaagcaatg attccttggg aaggattaaa tgatatgtat agagaagtag cctttcacgg
     5941 gggtattcct gatactggct tttatcgttt ctggatacaa ggtatttttg cgagatggac
     6001 agataatcca aatatcgaag acatgattca agcacaacaa gaacaccctt tgttcgatga
     6061 tttttggaaa cagcgtcaag tgccattatc acaaattaaa acacctctac taacatgtgc
     6121 tagttggtct acacaaggtt tgcacaaccg tggctctttt gaaggattta aacaagctgc
     6181 atcagaagaa aaatggctat atgtgcatgg acgtaaagag tgggaaagtt actacgctag
     6241 agaaaatctc gaacgccaaa aatcattctt tgatttttac cttaaagaag aaaataacga
     6301 ttggaaagat acgcctcatg tcatatatga agttagagat caattttata aaggcgaatt
     6361 caaatcagcg tcagcgttcc ctttacctaa cacagaatat acaccattgt atttaaatgc
     6421 tgaaaatcac acattgaatc atgcaaagat tagtagcgcg tatgtcgcac aatatgactc
     6481 tgaagataaa cgacaagatg taagttttaa atatacgttt gacaaagata ctgagttagt
     6541 tggaaacatg aacttaaaac tatgggtaag cactacagac tcagacgata tggatttatt
     6601 tgcaggtatt aaaaagttag atcgtcgtgg taatgaggtt aacttccctg attttaatca
     6661 tattgaaaat ggtcaagtag ctactggttg gttacgcgta tcacatcgtg aattagatca
     6721 agaaaaatcc acaatcgcgc aaccttggca taaacatgaa acagaattaa agttgtcaca
     6781 agatgagatt gtacctgttg aaatcgaatt gttaccttca ggcacgctat ttaaacaagg
     6841 cgaaacattg gaagttgttg taaaaggtag tgaaattgta attggtaata gtacccctgg
     6901 catgaaaaca cgttatgaac atgaagaaac cataaataac ggcatgcaca tgatttatac
     6961 tggtggtaaa tatgattcac aattaatcat tcctatcgtt aattgacatg caggaattac
     7021 ggtcgatttt gattaaaagt gacatagtga tagaagagaa ggccacacgc ttggaatctt
     7081 aaaccaaggt gtggcccttt ttattattga tggctattta attttataaa tcttgtcact
     7141 aaacttacta caagcaatat taaaccaata aataaagcca ttattgctgt attactttcg
     7201 ttgtttactc ctgtttttgg caattctttc aattgttgct tatgtactga cttgctcttg
     7261 ctatccaaat gaatactttg ttgtttagcc atttgtttat tactttttgt gtgaatatca
     7321 tttgctttaa tacttggatt tacagcacta ttattcgatg ttccatttgc gtttttcaac
     7381 tgattaataa catttggatc tttcgtgcca tctattaaat tatctggatg ttttagtaaa
     7441 tgtctgcagt cggatactat tttagaatct tttgccttta ccgcattatt cggcacctca
     7501 ttatcatctt tcaactgctt gataacattt ggatctttcg tgccatctat taaattatca
     7561 agttttttca cacgtgctat gtcactaact ggccctggat aatttgcatc cggttgtcta
     7621 actggtatta aattgctgtt ttcattcaca ccttcaggtt tgttattttc ggcgttcgca
     7681 ttatccgtaa aaatcaacac tgataaaatt gcaacaaata aacttataac ttttttcatc
     7741 ttattcatcc tttcctattt tactacctta atatcattgc tattaaagta acccatccat
     7801 ctattttttt cgtcgtataa agagtaatat ttatacccat ttggaatatt gtaatagagt
     7861 ttagctgtgt acgctttatc ttttttaatt aatttattat cacccttttt tgtaatttca
     7921 atgtttttcc aatatatata cttactttta attactttca ctttcttatt atatggatga
     7981 cttctatact tacctatcca ataatttatg taatctaatt ttaaatcatt tattctcacg
     8041 cctgaattat gattatatct gccgtatgca tgtactccga tgatattgtt ttttgaatct
     8101 cttatcgcac taccactttg tcctgaaaat gtgtctaccg tataatacat ttcattctca
     8161 tcaaaacttt ctaatttccc tttaccttta tattgtctgt tgtctgactt gtctcctgga
     8221 aatccagaaa tttcaatatt ttcacctttg ctaatgtgtg tgttcaaagt taagtaacct
     8281 gttttatttc ctaaattact atttaactta ataattgcta tatcttcttt tgtaggagca
     8341 ttat
//
LOCUS       DANBWH010000116         8336 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.119, whole genome shotgun sequence.
ACCESSION   DANBWH010000116 DANBWH010000000
VERSION     DANBWH010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8336
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.119"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            353..1312
                     /locus_tag="PPY92_001796"
     CDS             353..1312
                     /locus_tag="PPY92_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDJ2625786.1"
                     /translation="MNRNIVKLVVFMLILVVAVAGCGQKDTEEKTEMTTIKDELGTEK
                     IKKNPKRVVVLEYSFADYLAALDMKPVGIADDGSTKNITKSVRDKIGAYESVGSRPQP
                     NMEVISKLKPDLIIADVSRHKKIKSELSKIAPTIMLVSGTGDYNANIEAFKTVAKAVG
                     KEKEGEKRLEKHDKILAEIRKKIEQSTLKSAFAFGISRAGMFINNEDTFMGQFLIKMG
                     IQPEVTKDKTTHVGERKGGPYIYLNNEELANINPKVMILATDGKTDKNRTKFIDPAVW
                     KSLKAVKDNKVYDVDRNKWLKSRGIIASESMAEDLEKIAEKAK"
     gene            complement(1358..1489)
                     /locus_tag="PPY92_001797"
     CDS             complement(1358..1489)
                     /locus_tag="PPY92_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625787.1"
                     /translation="MRYVTMRQFIKRTVKTILVGYVIKFIRNKLSGKSSHPTDNKHN"
     gene            complement(1553..1768)
                     /locus_tag="PPY92_001798"
     CDS             complement(1553..1768)
                     /locus_tag="PPY92_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HDJ2625788.1"
                     /translation="MKVNKAIYVIVALFLGGLGIHKFYADKFGQGLFHLLFFWTGIPT
                     IISIIHAIFVIFTKKADKDGYIIFPKN"
     gene            1933..2484
                     /locus_tag="PPY92_001799"
     CDS             1933..2484
                     /locus_tag="PPY92_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDJ2625789.1"
                     /translation="MIRQARPEDRFDIAKLVYMVWDDMELELVKHLPKDMVLDAIEKS
                     CVDATYRTFYQHILVYEVENKVAGCIISYSGENELKYEKAWELLDLPEEIKQYGTPLP
                     VKEAKDDEYYIETIATFAAYRGRGIATKLLTSLLESNTHVKWSLNCDINNEAALKLYK
                     KVGFISDGQIELYKHMYHHLIVK"
     gene            complement(2536..3474)
                     /locus_tag="PPY92_001800"
     CDS             complement(2536..3474)
                     /locus_tag="PPY92_001800"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDJ2625790.1"
                     /translation="MSNQYQQYSTVKKYWHLMRPHTLTASVVPVLVGTAASKIYFLGS
                     EDHIKISLFIAMLLACLLIQAATNMFNEYYDYKKGLDDHESVGIGGAIVRNGMSPELV
                     LRLAIAFYILAAILGLFLAANSSFWLLPVGLVCMAVGYLYTGGPFPISWTPFGELFSG
                     VFMGMFIIVIAFFIQTGNIQSYVIWLSVPIVITIGLINMANNIRDRVKDKASGRKTLP
                     ILLGKNASLTFMAIMYFIAYAFIVLTIIIKPGGSLFYLLALLSFPMPVKVIRRFKKND
                     TPPTMMPAMAAAGKTNTFFGLLYALGIYISALFAGI"
     gene            3656..5017
                     /locus_tag="PPY92_001801"
     CDS             3656..5017
                     /locus_tag="PPY92_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HDJ2625791.1"
                     /translation="MATGVLEDDIVKEIYGSSKEWVSVEVKLSQSLDPSTLFHLTDNE
                     AGDRFYMRLNDNRTSYFGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHL
                     RVVGGFQFSSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEALNDL
                     MDLFNNISDIDVDEQIGEITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFD
                     KDISIPYILKQAYSKEKNSYIFLLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKR
                     SQDEDEDTKNVEAFLKDNKNLIEHRFVVDSILHDIKPYITELHYDKTPKILKNDHLYH
                     LYTEIKAPLKDDSYISLIDHLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYID
                     IYDDCEFIVAIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMN
                     GKS"
     gene            5004..6677
                     /gene="menD"
                     /locus_tag="PPY92_001802"
     CDS             5004..6677
                     /gene="menD"
                     /locus_tag="PPY92_001802"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDJ2625792.1"
                     /translation="MGNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEA
                     HPNIKTWIHPDERSAAFFAVGLIKGSERPVAILCTSGTAAANYTPAIAESQISRIPLI
                     VLTSDRPHELRSVGAPQAINQVNMFNNYVSYEFDMPIADDSKETINAIYYQMQIASQY
                     LYGPHKGPIHFNLPFRDPLTPDLNATELLTSEMKILPHYQKSIDASALRHILNKKKGL
                     IIVGDMQHQEVDQILTYSTIYDLPILADPLSHLRKFDHPNVICTYDLLFRSGLDLNVD
                     FVIRVGKPVISKKLNQWLKKTDAFQILVQNNDKIDVFPIAPDISYEISANDFFRSLME
                     DTTINRVSWLEKWQCLEKKGRKEIKCYLEQATDESAFVGELIKKTSEKDALFISNSMP
                     IRDVDNLLLNKNIDVYANRGANGIDGIVSTALGMAVHKRITLLIGDLSFYHDMNGLLM
                     SKLNNIQMNIVLLNNDGGGIFSYLPQKESATDYFERLFGTPTGLDFEYTAKLYQFDFK
                     RFNSVSEFKNATLLSETSTIYELITNREDNFKQHQILYQKLSEMIHDTL"
     gene            6664..7467
                     /gene="menH"
                     /locus_tag="PPY92_001803"
     CDS             6664..7467
                     /gene="menH"
                     /locus_tag="PPY92_001803"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDJ2625793.1"
                     /translation="MTHYKYYEANVETNQVLVLLHGFLSDSRTYYNHIDKYTDICHVI
                     TIDLPGHGEDQSSMDETWNFDYITTLLDRILDKYKDKSITLFGYSMGGRVALYYAING
                     HIPISNLILESTSPGIKEEANQLERRLVDDARAKVLDIAGIELFVNDWEKLPLFQSQL
                     ELPVEIQHQIRLQRLSQSPQKMAKALRDYGTGQMPNLWPRLKEIKVPTLILAGEYDEK
                     FVQIAKKMANLIPNSKCKLISATGHTIHVEDSDEFDTMILGFLKEEQND"
     gene            7460..8281
                     /gene="menB"
                     /locus_tag="PPY92_001804"
     CDS             7460..8281
                     /gene="menB"
                     /locus_tag="PPY92_001804"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDJ2625794.1"
                     /translation="MTNRQWETLREYDEIKYEFYEGIAKVTINRPEVRNAFTPKTVAE
                     MIDAFSRARDDQNVSVIVLTGEGDLAFCSGGDQKKRGHGGYVGEDQIPRLNVLDLQRL
                     IRIIPKPVIAMVKGYAVGGGNVLNVVCDLTIAADNAIFGQTGPKVGSFDAGYGSGYLA
                     RIVGHKKAREIWYLCRQYNAQEALDMGLVNTVVSLDKVEDETVQWCKEIMKHSPTALR
                     FLKAAMNADTDGLAGLQQMAGDATLLYYTTDEAKEGRDAFKEKRDPDFDQFPKFP"
BASE COUNT         3060 a         1168 c         1572 g         2536 t
ORIGIN      
        1 gaaagatgtt taagtcatca gttacttttt gtatttataa ataaagatta gtacagcata
       61 gtgcaaagat tatagggaaa tgaattaact attgcatcgc tatttatgaa tcgtaaacac
      121 tattatgaaa ttgcgagagt agtatggata attagaatga tggtttgaga taaatgagta
      181 ttattaacca gtgcgtcatc ttcaaaatta gaggccattt gctgtttata ataagaagat
      241 ttgataataa actatgataa gaaaaaataa tagcatacca aatatatcat aaacaaatta
      301 aatgataaaa taataatgca gttttctatt aaagttgttt ggaggtaaat atgtgaatag
      361 gaatatcgtt aaattagttg tgtttatgct aatcttagtt gtagcagtag cgggttgtgg
      421 tcaaaaagat actgaagaga aaactgaaat gacgacaata aaagatgaat taggaactga
      481 aaaaattaag aaaaatccta aacgtgttgt tgtattagaa tatagttttg ctgattattt
      541 agcagcatta gatatgaaac ctgttggtat tgcagatgat ggcagcacta aaaatataac
      601 aaagtcagta agagataaga ttggggcata tgaatcggtt ggatctagac cgcaaccgaa
      661 tatggaagtg ataagtaaat taaaaccgga tttgatcatt gcagatgtta gcagacataa
      721 gaaaatcaaa tcagaattga gcaaaattgc tccgacaatc atgttagtta gcggtacggg
      781 agattataat gcaaatattg aagcatttaa aacagtcgct aaagcagtag gcaaagagaa
      841 agaaggcgag aagcgtctgg aaaagcatga taaaatatta gcggagatta gaaagaaaat
      901 tgaacagagt acgttaaaat ctgcatttgc attcggtatc tcaagagcag gtatgtttat
      961 taataatgaa gatacattta tgggacaatt cttaattaaa atgggtattc aacctgaagt
     1021 cacaaaagac aaaactacgc atgttggtga acgcaagggt ggtccttata tatatttaaa
     1081 taatgaagaa cttgccaata tcaatccaaa agttatgatt ttagccactg acggaaaaac
     1141 ggacaaaaat agaacgaaat tcattgatcc tgcagtttgg aaatcattaa aagctgtgaa
     1201 agataacaaa gtttatgacg ttgaccgaaa taagtggttg aaatcaaggg ggattatcgc
     1261 aagtgaaagt atggcagaag atttagaaaa aattgcagaa aaagcaaaat aaaaatacag
     1321 cgctactcgt aaatcatata agagtggcgc tgtattttta attatgttta ttatctgtcg
     1381 gatgtgatga tttacctgaa agtttatttc gaataaattt aattacataa ccgacaagga
     1441 ttgttttaac agttctttta atgaattggc gcatcgttac atacctcatt tctctatatc
     1501 ttacgaacta tatacccatt catatatgct ttttaaacgt cattgtcaca atttaatttt
     1561 tagggaatat aatataacca tctttatctg cttttttagt aaaaatgaca aaaattgcat
     1621 gtattattga gatgatggta gggatacctg tccagaaaaa taataagtga aaaagacctt
     1681 gtccaaattt atcagcataa aatttatgaa tacctaaacc tccaagaaat aatgcaacaa
     1741 taacataaat ggctttattg actttcattt gtaaatcctc cttaactata attctactta
     1801 aattcgttgt gaaaaccaat atttctaact ttagaatttt caaactttct aaaattataa
     1861 gtatatcttt ttaaaataag ctagaatttc tatataataa atgttaataa cgtaaaaggg
     1921 aatgatgaca tagtgatacg tcaagcacgt ccagaggacc gatttgatat tgcgaagtta
     1981 gtttatatgg tttgggatga tatggaatta gaattggtaa agcatctacc taaagacatg
     2041 gtattagatg caattgaaaa aagctgtgtt gatgcaacat atcgaacttt ttatcagcat
     2101 attttagttt atgaagtaga aaataaagta gcaggttgta ttattagcta tagtggtgaa
     2161 aatgaattga aatacgaaaa agcatgggaa ctacttgact tgccagaaga aataaaacaa
     2221 tatggcacgc cattacctgt aaaagaagct aaagacgatg agtattatat agaaacaatt
     2281 gcgacatttg cagcatatag aggtagaggc atcgcgacaa agttattaac gtcattactt
     2341 gaatcaaata cacatgttaa atggagtttg aattgcgata ttaataatga agcagcatta
     2401 aagttatata aaaaagtagg ctttatatct gatggacaga ttgaattata caagcacatg
     2461 tatcatcatt taattgttaa ataaaaaaac tcgacagttc gatgtaagtc gattgccgag
     2521 tagtatcatt ttctattaaa tgcctgcaaa taatgcacta atataaatac ctaatgcata
     2581 taataaaccg aaaaatgtat ttgttttacc agcagcagcc attgctggca tcattgtagg
     2641 cggtgtatca ttcttcttga aacgtctgat aactttaaca ggcattggga atgataacaa
     2701 cgcaagtaag taaaataatg agccaccagg tttaataatg atcgtaagta caataaaggc
     2761 ataagcgata aagtacatga ttgccataaa tgttaaagaa gcatttttac ctaatagaat
     2821 gggtaaagtt ttgcgaccac ttgctttatc tttgacacgg tcgcgaatat tgttagccat
     2881 attaattaaa ccgatagtga ttactatagg tacacttaac caaattacat aactttgaat
     2941 attgccagtt tgaataaaga atgcaataac gataataaac atacccataa atacgcctga
     3001 gaataattca ccgaaaggcg tccatgaaat agggaaaggg ccacctgtat ataggtaacc
     3061 aacagccata catactaatc caactggtaa taaccaaaat gaagagttag cagctaaaaa
     3121 caaacctaat attgctgcta agatgtaaaa tgcaatggct aatcgtagca caagctctgg
     3181 gctcataccg ttgcgaacaa tggcaccacc aatgcctaca gattcatgat catcaaggcc
     3241 ttttttataa tcatagtatt cattaaacat attagttgct gcttgaataa gtaagcatgc
     3301 tagtaacatg gcaatgaata ggctgatttt aatatgatct tcgctaccaa gaaaatatat
     3361 tttagatgct gctgtaccaa ctaaaacggg tactacggaa gcagttaatg tatgaggacg
     3421 cattaaatgc caatatttct taactgtaga atattgctga tattgattac tcattgaata
     3481 caaaacctct ttaaatcata ttttttataa caaagatatt ttaatagaaa gtagcataaa
     3541 ggtcaattga cgtgaaaaat tgcaagataa gtttaagcaa ttcttgtaac acaattatcc
     3601 aaaaatgata caatataata atgagtgaaa aaagacgaag aaagaagtga aatggatggc
     3661 tacgggcgta ttagaggacg atattgtcaa agagatatat ggcagctcaa aggaatgggt
     3721 ttcagttgaa gtgaaattat cacagtcact agacccgagc acattatttc atctcactga
     3781 caatgaggca ggagatcgct tttatatgcg tttgaatgat aatcgaacgt catattttgg
     3841 ctacaaagca attcaattat tcaaaaataa ttctaaaaat aaacaatcta tttttaaaga
     3901 ctgggaaaaa ttaaaacata acatcacatt tatacatccg caatctgaga aacatcatct
     3961 tcgagttgtt ggagggtttc aattttcaag tcataaatca gatgatgaat ggcgagagtt
     4021 tggactaaat cattttgtat tacctgaagt tttaatttca actgataata atgggacatt
     4081 tttaacttat acagttaaaa gggaaagttt tactgttgag gcattgaacg atttaatgga
     4141 tttgttcaac aatatatcgg atatagatgt ggacgagcaa attggggaaa ttactagaaa
     4201 tgaagatatt tataaagatg actggcgtca acttgtagta gaagctatag aatctattaa
     4261 taatgaagaa aaaattgtac tagcacgtag acggttaata aagttcgata aagatatcag
     4321 tattccatat attctaaagc aagcatattc taaagaaaaa aacagttata tattcttgtt
     4381 agaatcacaa gattctattt tcttttcaca aacacctgaa caattaataa aggtcaataa
     4441 taaaatacta tcgactaaag ctgtagcagg tacaattaaa cgttcacaag atgaggacga
     4501 agatacaaaa aatgttgaag catttttaaa agataataaa aacttaatcg aacatcgatt
     4561 tgttgttgac agtattttac atgatattaa accttatatc actgaattac attatgataa
     4621 gacgcctaaa attctaaaaa atgatcattt atatcacttg tacactgaaa taaaggcgcc
     4681 actgaaggat gattcgtata ttagtttaat tgatcattta catccaacac ctgctttagg
     4741 tggctatcca aaagaatttg cgatggattt tattgaacag aaagaatttg gtacacgagg
     4801 attatatggt gcgccggttg gctatataga tatatatgat gattgtgaat ttattgttgc
     4861 aattcgttcg atgcttatta agaaagcaca agcaacttta tttgctgggt gtggcattgt
     4921 taaagattct gatccagata gtgaattggc agaaacgaac cttaagttca cacctatgat
     4981 gaatgcatta ggagtcgata tgaatgggaa atcataaagc agctttaacg aagcaagttt
     5041 ttacatttgc atctgagtta tatgcatacg gcgtaaggga agtagttatc agtccgggat
     5101 cacgctcaac gccacttgca cttgcatttg aagcacatcc aaatattaaa acatggatac
     5161 accccgatga gcgaagtgca gcattttttg cagttgggtt aattaaaggc agtgaaagac
     5221 ctgtcgctat attatgtacg tcaggtacag cagcagcgaa ttatacgcct gcaattgctg
     5281 aaagccaaat tagtagaatt ccattaatcg ttttaacaag tgaccgtccg catgaattaa
     5341 gaagtgtagg cgcaccacaa gcgattaatc aagtaaatat gtttaataat tatgtaagtt
     5401 atgagttcga tatgcctatt gcggatgata gtaaagagac cattaatgca atttattatc
     5461 aaatgcaaat tgctagtcaa tatttatatg gaccacataa agggccaatt cattttaact
     5521 tgccatttag agatccgtta acacctgatt tgaatgcaac agaattgtta acttctgaga
     5581 tgaagatttt accgcactat caaaaaagta tagatgcatc ggcattaaga cacattttaa
     5641 ataagaaaaa aggtttaatt attgtagggg atatgcagca ccaagaagtt gatcaaatac
     5701 taacgtattc aacgatatat gatttgccta ttttagctga tcctttaagt catttaagaa
     5761 aatttgatca tccgaatgtt atctgtacat atgatttgct gtttagaagc ggcttagact
     5821 taaatgtgga tttcgtaatt cgtgttggga aaccagtgat ttctaaaaag ttgaatcaat
     5881 ggttaaagaa aactgatgca tttcaaatat tagtgcaaaa caatgataag attgatgtct
     5941 ttcctatagc gccagatatt tcatatgaga tttctgcaaa tgatttcttt agatcattaa
     6001 tggaagacac gaccatcaat cgcgtaagtt ggttagaaaa atggcaatgc ttagagaaaa
     6061 aagggcgtaa agaaattaaa tgttatttgg aacaagctac agatgagagt gcattcgttg
     6121 gtgaattgat taagaaaaca tctgaaaaag atgcattatt tattagtaat agtatgccta
     6181 tcagagatgt agataacttg ttattgaata aaaatataga tgtctatgcg aatcgtggtg
     6241 cgaatggtat tgatggtatc gtttcaactg cactgggtat ggctgtgcat aaacgaataa
     6301 cattattgat aggtgatttg tcattttatc atgatatgaa tggactatta atgtcaaaat
     6361 taaataatat tcagatgaat attgtattat tgaacaacga tggtggcggt attttttcat
     6421 atttaccaca aaaagaaagt gcaactgact attttgaacg gttgtttggc acaccgacgg
     6481 gattggattt cgagtataca gctaagttat atcaattcga ttttaaacgt tttaacagtg
     6541 tttcagaatt taaaaatgcc acattgttat ctgaaacttc gacgatttat gaattgataa
     6601 cgaatcgcga agataacttt aaacagcatc aaattttata tcagaaattg agtgaaatga
     6661 ttcatgacac attataaata ctatgaagca aacgttgaga ccaatcaagt tttagtatta
     6721 ctgcatggtt ttcttagcga cagtcgtact tattataatc acatcgataa atatactgat
     6781 atctgtcatg tcatcactat agacttacca ggccatggcg aagatcagtc ttcaatggat
     6841 gaaacgtgga attttgatta tattacgacg ttgttagacc gaattttaga taaatataaa
     6901 gataaatcaa taacattgtt tggatattca atgggtgggc gtgttgcatt atattatgca
     6961 attaatggtc acatccctat atctaatttg atattagaaa gtacgtcacc aggtattaaa
     7021 gaagaagcaa atcaattgga acgccgtctt gttgatgatg cacgtgctaa agtattagac
     7081 atagcaggta ttgaattatt tgttaatgat tgggaaaagt tgccattatt tcaatcgcaa
     7141 ctagaattac cagttgaaat acaacatcaa ataagactac aacgattgtc tcaatcgcca
     7201 cagaaaatgg ccaaagcatt aagagattat ggtacaggtc aaatgccaaa cttatggccg
     7261 cgcctgaaag aaattaaagt accaacatta atattagctg gagaatatga tgaaaaattt
     7321 gtacagattg cgaaaaaaat ggcaaattta attcctaata gtaaatgtaa attaatttct
     7381 gctacaggtc atacaattca tgtggaagat agtgatgaat ttgatacaat gatattagga
     7441 tttttaaagg aggagcaaaa tgactaacag acaatgggaa acacttagag aatatgatga
     7501 aatcaaatat gaattttacg aagggattgc taaggtaaca ataaatcgcc ctgaagtacg
     7561 caatgcgttt acacctaaaa cagttgctga aatgattgac gcattttcac gtgcacgtga
     7621 tgatcaaaac gtttcagtta tcgtattaac tggtgaaggt gatttagcat tctgttctgg
     7681 tggtgaccag aagaaacgtg gacatggtgg ttatgtaggt gaagaccaaa tccctcgctt
     7741 aaatgtatta gatttacagc gtttaattcg tattattcca aaaccggtta tcgcgatggt
     7801 aaaaggttat gctgtaggtg gcggtaatgt actaaatgtt gtttgtgact taacgattgc
     7861 tgctgataat gctatttttg gacaaactgg tcctaaagta ggttcatttg atgcaggtta
     7921 tggttcagga tatttagcac gtatcgttgg acataagaaa gcacgtgaaa tctggtactt
     7981 atgtcgtcaa tacaatgcac aagaagcttt agatatgggc ctagtaaata cagtggtatc
     8041 tttagataaa gttgaagatg aaactgtgca atggtgtaaa gagattatga aacactcacc
     8101 aacagcttta cgattcctta aagcagctat gaatgctgac acagatggtt tagctggttt
     8161 acaacaaatg gctggggatg caacattgct ttattacaca actgatgaag cgaaagaagg
     8221 ccgtgatgcg tttaaagaaa aacgtgatcc tgacttcgat caattcccta aattcccata
     8281 agttattttg aatatgatat taagtcactt gcgtcgttta ttagcgacga taggtg
//
LOCUS       DANBWH010000117         8287 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.120, whole genome shotgun sequence.
ACCESSION   DANBWH010000117 DANBWH010000000
VERSION     DANBWH010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8287
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.120"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            38..688
                     /locus_tag="PPY92_001805"
     CDS             38..688
                     /locus_tag="PPY92_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDJ2625795.1"
                     /translation="MGVHQYFKRLSDMERLIRLPGKFKYFEHNVAAHSFKVTKIAQYL
                     ATVEEYHGRKINWKSLYEKALNHDFAEVFTGDIKTPVKYASSELKKLFSQVEEEMVET
                     FIEEEIPLQYRDVYKQRLQEGKDDSLEGQILSVADKIDLLYETFGEIQKRNPEELFFE
                     IYEMSLETIIQFDHLASVQDFINNIIPEMLTENFIPRTELRETTMNILNKRKEENE"
     gene            685..921
                     /locus_tag="PPY92_001806"
     CDS             685..921
                     /locus_tag="PPY92_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbA family protein"
                     /protein_id="HDJ2625796.1"
                     /translation="MIWYFSAAFFPCVLVVLFSVITRSKWVGTILTLILIGASIYKEY
                     FHNEWIIFIDVVSLLAGYLIIDQLEFHKHQDEDR"
     gene            1190..3175
                     /gene="uvrB"
                     /locus_tag="PPY92_001807"
     CDS             1190..3175
                     /gene="uvrB"
                     /locus_tag="PPY92_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002473941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDJ2625797.1"
                     /translation="MVEHYPFKIHSDFEPQGDQPQAIKEIVDGIKAGKRHQTLLGATG
                     TGKTFTMSNVIKEVGKPTLIIAHNKTLAGQLYSEFKEFFPENRVEYFVSYYDYYQPEA
                     YVPSTDTFIEKDASINDEIDQLRHSATSALFERDDVIIIASVSCIYGLGNPEEYKDLV
                     VSVRVGMEMDRSELLRKLVDVQYTRNDIDFQRGTFRVRGDVVEIFPASKEELCIRVEF
                     FGDEIDRIREVNYLTGEVLKEREHFAIFPASHFVTREEKLKVAIERIEKELEERLKEL
                     RDENKLLEAQRLEQRTNYDLEMMREMGFCSGIENYSVHLTLRPLGSTPYTLLDYFGDD
                     WLVMIDESHVTLPQVRGMYNGDRARKQVLVDHGFRLPSALDNRPLKFEEFEEKTKQLV
                     YVSATPGPYEIEHTDKMVEQIIRPTGLLDPKIEVRPTENQIDDLLSEIQTRVERNERV
                     LVTTLTKKMSEDLTTYMKEAGIKVNYLHSEIKTLERIEIIRDLRMGTYDVIVGINLLR
                     EGIDIPEVSLVVILDADKEGFLRSNRSLIQTIGRAARNDKGEVIMYADKMTDSMKYAI
                     DETQRRREIQMKHNEKHGITPKTINKKIHDLISATVENDENNDKAQTVIPKKMTKKER
                     QKTIDNIEKEMKQAAKDLDFEKATELRDMLFELKAEG"
     gene            3183..6029
                     /gene="uvrA"
                     /locus_tag="PPY92_001808"
     CDS             3183..6029
                     /gene="uvrA"
                     /locus_tag="PPY92_001808"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDJ2625798.1"
                     /translation="MKEPSIVVKGARAHNLKDIDIELPKNKLIVMTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMDKPDVDTIEGLSPAISIDQKTTSKNPRSTVAT
                     VTEIYDYIRLLYARVGKPYCPNHNIEIESQTVQQMVDRIMELEARTKIQLLAPVIAHR
                     KGSHEKLIEDIGKKGYVRLRIDGEIVDVNDVPTLDKNKNHTIEVVVDRLVVKDGIETR
                     LADSIETALEISEGQLTVDVIDGEDLKFSESHACPICGFSIGELEPRMFSFNSPFGAC
                     PTCDGLGQKLTVDVDLVVPDKDKTLNEGAIEPWIPTSSDFYPTLLKRVCEVYKINMDK
                     PFKKLTERQRDILLYGSGDKEIEFTFTQRQGGTRKRTMVFEGVVPNISRRFHESPSEY
                     TREMMSKYMTELPCETCHGKRLSREALSVYVGGLNIGEVVEYSISQALNYYKNIDLSE
                     QDQAIANQILKEIISRLTFLNNVGLEYLTLNRASGTLSGGEAQRIRLATQIGSRLTGV
                     LYVLDEPSIGLHQRDNDRLINTLKEMRDLGNTLIVVEHDDDTMRAADYLVDIGPGAGE
                     HGGQIVSSGTPQKVMKDKKSLTGQYLSGKKRIDVPEYRRPASDRKISIRGARSNNLKG
                     IDVDIPLSIMTVVTGVSGSGKSSLVNEVLYKSLAQKINKSKVKPGLYDKIEGIDQLDK
                     IIDIDQSPIGRTPRSNPATYTGVFDDIRDVFAQTNEAKIRGYQKGRFSFNVKGGRCEA
                     CKGDGIIKIEMHFLPDVYVPCEVCDGKRYNRETLEVTYKGKNIADILEMTVEEATQFF
                     ENIPKIKRKLQTLVDVGLGYVTLGQQATTLSGGEAQRVKLASELHKRSTGKSIYILDE
                     LTTGLHVDDISRLLKVLNRLVENGDTVVIIEHNLDVIKTADYIIDLGPEGGSGGGTIV
                     ATGTPEDIAQTKSSYTGKYLKEVLERDKQNTEDK"
     gene            6693..7625
                     /locus_tag="PPY92_001809"
     CDS             6693..7625
                     /locus_tag="PPY92_001809"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDJ2625799.1"
                     /translation="MLTTEKLVETLKLDLIAGEEGLSKPIKNADISRPGLEMAGYFSH
                     YASDRIQLLGTTELSFYNLLPDKDRAGRMRKLCRPETPAIIVTRGLQPPEELVEAAKE
                     LNTPLIVAKDATTSLMSRLTTFLEHALAKTTSLHGVLVDVYGVGVLITGDSGIGKSET
                     ALELVKRGHRLVADDNVEIRQINKDELIGKPPKLIEHLLEIRGLGIINVMTLFGAGSI
                     LTEKRIRLNINLENWNKQKLYDRVGLNEETLSILDTEITKKTIPVRPGRNVAVIIEVA
                     AMNYRLNIMGINTAEEFSERLNEEIIKNSHKSEE"
     gene            7631..>8287
                     /locus_tag="PPY92_001810"
     CDS             7631..>8287
                     /locus_tag="PPY92_001810"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDJ2625800.1"
                     /translation="MGIVFNYIDPVAFNLGPLSVRWYGIIIAVGILLGYFVAQRALVK
                     AGLHKDTLVDIIFYSALFGFIAARIYFVIFQWPYYAENPSEIIKIWHGGIAIHGGLIG
                     GFIAGVIVCKVKNLNPFQIGDIVAPSIILAQGIGRWGNFMNHEAHGGPVSRAFLEQLH
                     LPNFIIENMYINGQYYHPTFLYESIWDVAGFIILVNIRKHLKLGETFFLYLTWYSIGR
                     F"
BASE COUNT         2985 a         1137 c         1674 g         2491 t
ORIGIN      
        1 tataatttat gattaattaa tggaggaggt aattgaaatg ggtgtacatc aatattttaa
       61 aagattatca gatatggaaa gacttataag attacctgga aaatttaaat attttgaaca
      121 caatgttgca gcacactcct ttaaagtaac taaaattgct caatatctag caacagttga
      181 agaatatcat ggtcgaaaga ttaattggaa aagcttatat gaaaaagcat taaatcatga
      241 tttcgctgaa gtgtttactg gtgatataaa aacacctgtt aaatatgcga gtagtgaatt
      301 aaaaaaatta ttttcgcaag ttgaagaaga aatggtagag acctttattg aagaagaaat
      361 tccattacaa tatagagatg tttataagca acgactgcaa gaaggtaaag atgattcatt
      421 agaaggccaa atactttcag ttgctgataa aattgatttg ctttatgaaa catttggaga
      481 aatacaaaaa cgtaatcccg aagaattatt tttcgaaatt tatgaaatga gtctagaaac
      541 aattattcaa tttgaccatt tagcatctgt acaagatttt attaataata tcattccaga
      601 aatgttgact gaaaacttta tacctagaac agaattaaga gaaacaacca tgaacatttt
      661 aaataaaaga aaagaggaaa atgaatgata tggtatttta gcgcagcatt ctttccatgt
      721 gtcttggtag tattatttag tgtaataaca agaagtaaat gggtcggtac tattctgaca
      781 ttaattttaa ttggtgcctc aatctataaa gagtatttcc ataacgagtg gattattttt
      841 attgatgtag tgtcattatt agctggttat ttaattatag atcaactcga atttcataaa
      901 catcaagatg aagatcgcta agattaactt taaaataatg tttcaaacaa atttgttgaa
      961 acaaaacgat gattaatata atgtgtattt acatactaaa aataacaaga taaacgattt
     1021 gatttaaggc aagcatagtt agcacaacta tgtttgtttt tctttgttcg acatttttac
     1081 gaacaaacgt ttgctttttg tgtggctact ttgctaaaat atgtaatgag aaagcgaaga
     1141 gttgagcgga aatataaata atacgttgaa agaggaggca tatgtgacaa tggttgaaca
     1201 ttatcctttt aaaatacatt ctgattttga gcctcaaggt gaccaaccgc aagcaattaa
     1261 ggaaatcgtg gatggtatta aagcggggaa aagacatcaa actttattag gtgcaactgg
     1321 cacagggaaa acatttacga tgagtaatgt tattaaagaa gttgggaaac caacgttaat
     1381 tatcgcacat aacaaaacat tagcagggca attatatagt gagtttaaag aattttttcc
     1441 tgaaaacagg gtggaatact ttgtaagtta ctatgattat tatcaacctg aggcatacgt
     1501 accgtctact gacactttta ttgaaaaaga tgcctcaatc aatgatgaaa ttgatcaact
     1561 acgacattct gctacaagtg cattatttga acgcgatgat gtaattatta ttgctagtgt
     1621 aagttgtata tatggtttag gtaatcctga agaatataaa gatttagtag taagtgttcg
     1681 agttggtatg gaaatggata gaagtgaatt acttagaaaa cttgtagatg tgcaatatac
     1741 acgaaatgac atcgatttcc aacgaggaac gtttcgagtg cgtggtgatg tagttgaaat
     1801 attcccagcc tctaaagaag aactttgtat aagggttgag tttttcggcg atgagattga
     1861 ccgtatccga gaagttaact acctaacagg tgaagtgttg aaagaaagag aacattttgc
     1921 gatattccca gcttctcact tcgtaacacg tgaagaaaag ttgaaagttg cgattgaacg
     1981 tattgaaaaa gaattggaag aacgattgaa agaattacga gatgagaata aattactaga
     2041 agcgcaaagg ttagaacagc gtaccaacta tgatttagaa atgatgcgag agatgggatt
     2101 ctgttcagga attgaaaact attccgtaca tttaactttg cgaccactgg gttcgacacc
     2161 gtatacttta ttggattact ttggcgatga ttggttagta atgattgatg aatcacatgt
     2221 gacattacca caagttcgag gcatgtataa cggagacaga gcgcgtaaac aagttttggt
     2281 ggatcatgga tttagattac cgagtgcatt agataaccgt ccacttaaat ttgaagaatt
     2341 tgaagaaaag acaaaacaac ttgtgtatgt atctgcaacg cctggaccat acgaaattga
     2401 acatacggat aagatggttg aacaaattat tcgtcctact ggtttactgg atcctaaaat
     2461 tgaggttaga cctactgaaa atcaaattga cgatttatta agtgaaattc aaacaagggt
     2521 tgagcgtaat gaacgcgtac ttgttacaac gctcactaaa aagatgagtg aagatttaac
     2581 cacatacatg aaagaagcgg gtattaaagt taattatctg cattcagaaa tcaagacatt
     2641 agaacgaatc gaaataatta gagacttacg aatgggtaca tatgatgtta tcgtaggtat
     2701 taatttatta agagagggta ttgatatacc agaagtttct ctagttgtca tattagatgc
     2761 agataaagaa ggatttttac gttctaaccg ctcattaatt caaacaatag gtagagctgc
     2821 gcgtaacgat aaaggtgaag tcattatgta tgccgataaa atgactgatt cgatgaagta
     2881 tgcaattgat gagacacaac gtcgtcgaga aatacagatg aaacataatg aaaaacatgg
     2941 tattacacct aaaacaatta ataaaaaaat acatgattta attagtgcta ctgttgaaaa
     3001 tgacgaaaat aatgacaaag cacaaactgt gatacctaag aagatgacga aaaaagaacg
     3061 tcaaaagaca atcgacaata tagaaaaaga aatgaaacaa gcagcgaaag atttagattt
     3121 cgagaaagct acagaattaa gagatatgtt atttgaatta aaagcagaag ggtgacaagt
     3181 aaatgaaaga accatccata gtagtaaaag gtgctcgtgc gcataacttg aaagatattg
     3241 atatcgaact acctaaaaat aaattaattg ttatgacagg tttatctggg tcaggtaaat
     3301 cgtcattagc attcgatact atatatgctg aaggacaacg acgttatgtt gaatcattaa
     3361 gtgcctatgc gcgtcaattt ttaggccaaa tggacaaacc agatgttgat acaattgaag
     3421 gattatcgcc agcaatttca atagatcaaa aaacaacaag taaaaatcca agatcaactg
     3481 tagcaacagt aacagaaata tatgattata tacgtttgtt atatgcacgt gttggtaaac
     3541 cttactgtcc aaatcacaat atagaaattg aatcgcaaac agtacaacaa atggttgacc
     3601 gcattatgga attagaggca cgtacaaaga ttcaattatt agcacctgtc atcgctcatc
     3661 gtaaaggtag tcatgaaaag ctaatcgaag atattggtaa aaaaggttat gtacgtttaa
     3721 gaatcgatgg cgaaattgtt gatgtaaatg atgtacctac tttagataag aacaagaatc
     3781 atacaataga agttgttgta gaccgattag ttgttaaaga tggaattgaa acacgactag
     3841 ctgactctat agaaactgcg ttagagattt cagaaggaca attaacagtt gatgtcattg
     3901 acggggaaga ccttaagttt tcagaaagcc atgcttgtcc tatatgtgga ttttcaatcg
     3961 gagagttaga accaagaatg tttagcttta acagtccttt tggtgcttgt ccgacatgtg
     4021 atggcttagg ccaaaagtta acagtcgatg tagacttggt tgttcccgac aaagataaga
     4081 cgctaaacga aggtgcaata gaaccttgga taccgacgag ttctgatttt tatccaacat
     4141 tgttaaaacg tgtttgtgaa gtttataaaa tcaatatgga taaacctttt aaaaagttaa
     4201 cagaacgtca acgtgatatt ttattgtatg gttctggtga caaagaaatt gaatttacat
     4261 ttacacaacg tcaaggtggt actagaaaac gaacaatggt tttcgagggt gtagttccta
     4321 atataagtag acgattccat gaatctcctt cagaatatac acgtgaaatg atgagtaaat
     4381 atatgaccga actaccttgt gaaacttgtc atggaaagcg attgagtcgt gaagcgttat
     4441 ctgtttatgt aggtggttta aatattggtg aagtagtcga atattcaatc agtcaagcgc
     4501 tgaactatta taaaaacatt gatttgtcag aacaagatca agcgattgca aatcaaatat
     4561 tgaaagaaat tatttcccga ctcacttttt taaataatgt gggacttgaa tatttaacgc
     4621 taaacagagc ttcaggtaca ctttcaggtg gtgaagcaca acgtattcga ttggcaacgc
     4681 aaattgggtc gcgtttgact ggtgtcttat atgtattaga tgagccatca attggactgc
     4741 atcaaagaga taatgatcga ttaattaata cacttaaaga aatgagagat ttaggaaata
     4801 ctttaattgt agttgaacac gatgatgata caatgcgtgc ggctgattac ttagtggata
     4861 tagggcctgg tgctggtgaa catggaggac aaattgtgtc tagtgggaca cctcaaaagg
     4921 taatgaaaga taaaaaatca ttaacaggac aatacttgag tggtaagaaa cgtattgacg
     4981 tacctgaata tcgcagaccg gcttcagatc gtaaaatttc tatacgtgga gctagaagca
     5041 acaatcttaa agggattgat gtggacatac cactatcaat catgacggtt gttacaggtg
     5101 tatcaggttc tggtaaaagc tcattagtaa atgaagtatt atacaaatca ttagctcaaa
     5161 aaattaataa atctaaagta aagccaggat tgtacgataa gattgaaggt attgatcaac
     5221 ttgataaaat tattgatatt gatcaatcac cgataggtag aacgccacgc tctaatccag
     5281 caacatatac tggtgtgttt gatgatatac gtgatgtgtt tgcgcaaaca aatgaagcta
     5341 aaattcgagg atatcaaaaa gggcgtttta gttttaatgt aaaaggtgga cgctgtgaag
     5401 cttgtaaagg tgacggtatt attaaaattg aaatgcattt tttacctgat gtttatgttc
     5461 cttgtgaagt gtgtgatggt aaacgatata atcgtgagac actagaggtt acttacaaag
     5521 gtaaaaatat tgctgacatt ttagaaatga ctgttgaaga agcaacacaa ttttttgaaa
     5581 atattcctaa gattaagcgc aagttacaaa cactagttga tgttggtctt ggatacgtca
     5641 cattaggtca acaagctaca acgttatcag gtggtgaggc tcaacgtgtg aaacttgcat
     5701 ctgaacttca taaacgttca actggtaaat ctatttatat cctagacgaa ctaacaacag
     5761 ggttacatgt tgacgatatt agcagattat taaaagtatt aaatcgatta gttgaaaatg
     5821 gtgacactgt tgtaattatt gaacataacc tagatgttat caaaacagca gactatatta
     5881 tcgacttagg tcctgaaggt ggtagtggcg gtggtactat tgttgcgact ggcacacccg
     5941 aagatattgc tcagacaaag tcatcatata caggaaagta tttaaaagaa gtacttgaac
     6001 gagataaaca aaatactgaa gataaataag attaaaagaa gtgaagggtg ttgtaaattt
     6061 acccttcact tctttttatt aatttagtaa tgaatagtag aaagaaaaga tgcgtaaaaa
     6121 gaattatgtt aagatagggt caatctagag ttgctaaaca taaatcgaaa ctgagagtgg
     6181 gacagaaatg ataaagaatc actaatgatt tattatgtag tggttctttg tcattagcca
     6241 cagctattgt gtacttaaaa ataggaatgc atgagtgcaa ctcatgcata agaaatacta
     6301 atttctaaag aaaaagtatt tctttatgtt ggggcccacc ccaacttgca ttgtttgtag
     6361 aatttctttt cgaaattctc tgtgttgggg ccccgccaac ttgcattgcc tgtagaattt
     6421 cttttcgaaa ttctttatgt tggggccccg ccaactaatt acaatatatc attgtagagc
     6481 ttaggtcatt gatttatggc tcggactttt atggcgatat gaaccatgta aattaagcaa
     6541 gcaataaatt aatgattgat attgacttgt aaaataataa caataatgaa caattaatat
     6601 ttattttagc ttttcaatgt agattggtgt tatatttttg atatgataag aagagatgta
     6661 agagtaggga taaatacaat tgaggtgaac ccatgttaac gacagaaaaa ctagttgaaa
     6721 cattaaagtt agatttaatc gctggtgaag aaggactatc gaagccaatt aaaaatgctg
     6781 atatatcaag accaggctta gagatggcag gttatttttc acattatgcg tcagatagaa
     6841 tacaactatt aggaacaacg gaactatcgt tttacaattt attaccagat aaggatcgcg
     6901 caggtcgtat gcgtaaacta tgcagaccag aaacgcctgc aattattgtg acacgtggat
     6961 tgcagccacc agaagaatta gttgaagctg caaaagaatt aaatacccca cttatagttg
     7021 ctaaagatgc gactacaagt ttaatgagtc gcttaacaac gtttttagag catgcacttg
     7081 caaagacgac atctttacat ggtgttttag tagatgttta cggtgttggt gtactaatta
     7141 ccggtgattc aggaataggt aaaagtgaga ctgcgttgga attagttaaa cgtgggcata
     7201 gattagtagc agatgataat gtagaaatac gtcaaattaa taaagatgaa ctaataggga
     7261 aaccaccaaa gttaatagaa catctattag aaatacgtgg actaggtatt atcaatgtta
     7321 tgactttatt tggcgcgggt tcaatattaa ctgaaaaacg aattagatta aatattaatt
     7381 tggaaaactg gaacaagcaa aagttatatg accgcgtagg tcttaatgaa gagacgctaa
     7441 gtattttaga tactgaaatc actaaaaaaa caatacctgt aagacctggt agaaatgttg
     7501 cggtaattat tgaggtcgct gcaatgaact atcgattaaa tatcatgggc attaacactg
     7561 ccgaagaatt tagtgaaaga ttaaatgaag aaattatcaa gaacagtcat aagagtgagg
     7621 agtaggttga atgggtattg tatttaacta tatagatcct gtggcattta acttaggacc
     7681 actgagtgta cgatggtatg gaattatcat tgctgtcgga atattacttg gttactttgt
     7741 tgcacaacgt gcactagtta aagcaggatt acataaagat actttagtag atattatttt
     7801 ttatagtgca ctatttggat ttatcgcggc acgaatctat tttgtgattt tccaatggcc
     7861 atattacgcg gaaaatccaa gtgaaattat taaaatatgg catggtggaa tagcaataca
     7921 tggtggttta ataggtggct ttattgctgg tgttattgta tgtaaagtga aaaatttaaa
     7981 cccatttcaa attggtgata tcgttgcgcc aagtataatt ttagcgcaag gaattggacg
     8041 ctggggtaac tttatgaatc acgaggcaca tggtggaccg gtgtcacgcg cttttttaga
     8101 acaattacat ttgcctaatt ttataataga aaatatgtat attaacggcc aatattatca
     8161 tccaacattc ttatatgaat ccatttggga tgtcgctgga tttattatct tagttaatat
     8221 tcgtaaacat ttaaaattag gagaaacatt ctttttatat ttaacttggt attcaattgg
     8281 tcgattc
//
LOCUS       DANBWH010000118         8237 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.121, whole genome shotgun sequence.
ACCESSION   DANBWH010000118 DANBWH010000000
VERSION     DANBWH010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8237
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.121"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(23..1219)
                     /locus_tag="PPY92_001811"
     CDS             complement(23..1219)
                     /locus_tag="PPY92_001811"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDJ2625801.1"
                     /translation="MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVAC
                     ETTVTTGMALIAGEISTTTYVDIPKVVRETIKEIGYTRAKYGYDYETMAILTAIDEQS
                     PDIAQGVDKALEYRDKDSEEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKR
                     LSDVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHADDVTLEQIQEDIKA
                     HVIYPTVPENLINEQTKFYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCF
                     SGKDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAEPVSIAIDTFGTGKV
                     SEGQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEEL
                     KDAVKY"
     regulatory      complement(1325..1436)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1591..3183
                     /gene="pckA"
                     /locus_tag="PPY92_001812"
     CDS             1591..3183
                     /gene="pckA"
                     /locus_tag="PPY92_001812"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDJ2625802.1"
                     /translation="MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTE
                     LGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGEINQPIDEETFLKLYHKVLDYLDK
                     KDELYVFKGYAGSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVS
                     APHFKADPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMS
                     MHCSANVGEKGDVALFFGLSGTGKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAK
                     AINLSKEKEPQIFDAIKYGAILENTVVAEDGSVDFEDNRYTENTRAAYPINHIDNIVV
                     PSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSF
                     STCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMV
                     NQAISGKLKNAEYTKDSTFGLSIPVEIEDVPKTILNPINAWSDKEKYKAQAEDLIQRF
                     EKNFEKFGEKVEHIAEKGSFNK"
     gene            complement(3561..4307)
                     /locus_tag="PPY92_001813"
     CDS             complement(3561..4307)
                     /locus_tag="PPY92_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDJ2625803.1"
                     /translation="MPIESFTHQFEEITYLSDDLQVKALMMTPHHEVNRIVVYLRGGK
                     GQVGRVRAGRLMQFSDSQTLVIGPYYRGNNGSEGKDEFYRGDLNDVTQLLRLLHDKYP
                     HAFIHMVGFSRGGLQGLLTFQDLPVTSYTIWGGVSDIDLMYEERVDLRGMLRRMIGHP
                     KKDRAAYEARQAIPNINENSPPILIVHGGKDQQVGIHHAYYLADQLELKGATHETFYQ
                     MAEGHVPRPPAMVETLTYIKEFMKQVELHS"
     gene            complement(4312..4785)
                     /gene="ytkD"
                     /locus_tag="PPY92_001814"
     CDS             complement(4312..4785)
                     /gene="ytkD"
                     /locus_tag="PPY92_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase YtkD"
                     /protein_id="HDJ2625804.1"
                     /translation="MKFRDKDNRQVNLTFKKDNEIADGNHVLAIPTFKNQLLFTKHNL
                     RGIEFPGGKRERGESSAEAVTRELYEETGAKVKNIHYIAQYTIETHDQTDFVKDVYFI
                     EVESLVSKNDYLETAGPVLFNCINDIELAQRSFLLQDSTILKCVERVQSLGFYQT"
     gene            4851..5108
                     /gene="yidD"
                     /locus_tag="PPY92_001815"
     CDS             4851..5108
                     /gene="yidD"
                     /locus_tag="PPY92_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDJ2625805.1"
                     /translation="MKKIFLAMIHFYQRFISPLTPPTCRFYPTCSEYTREAIQYHGAF
                     KGLYLGIRRILKCHPLHKGGFDPVPLKKDKSASKHSHKHNH"
     gene            complement(5105..6106)
                     /gene="menC"
                     /locus_tag="PPY92_001816"
     CDS             complement(5105..6106)
                     /gene="menC"
                     /locus_tag="PPY92_001816"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDJ2625806.1"
                     /translation="MKLTALHFYKYSEPFKSQIVTPKVTLTHRDCLFIELIDDKGNAY
                     FGECNAFQTDWYDHETIASVKHVIEQWFEDNRNKSFETYEAALKLVDSLENTPAARAT
                     IVMALYQMFHVLPSFSVAYGATASGLSNKQLESLKATKPTRIKLKWTPQIMHQIRVLR
                     ELDFHFQLVIDANESLDRQDFTQLQLLAREQVLYIEEPFKDISMLDEVVDGTIPPIAL
                     DEKATSLLDIINLIELYNVKVVVLKPFRLGGIDKVQTAIDTLKSHGVKVVIGGMYEYG
                     LSRYFTAMLARKGDYPGDVTPAGYYFDQDVVTKSGILKEGRLEFRPPLVDITQLQPY"
     gene            complement(6111..7589)
                     /gene="menE"
                     /locus_tag="PPY92_001817"
     CDS             complement(6111..7589)
                     /gene="menE"
                     /locus_tag="PPY92_001817"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDJ2625807.1"
                     /translation="MDFWLYKQAQQNGHHIAITDGQESYTYQNLYCEASLLAKRLKAY
                     QQSRVGLYIDNSIQSIILIHACWLANIEIAMINTRLTPNEMTNQMRSIDVQLIFCTLP
                     LELRGFQIVSLDDIEFAGRDITTNGLLDNTMGIQYDTSNETVVPKESPSNILNTSFNL
                     DDIASIMFTSGTTGPQKAVPQTFRNHYASAIGCKESLGFDRDTNWLSVLPIYHISGLS
                     VLLRAVIEGFTVRIVDKFNAEQILTMIKNERITHISLVPQTLNWLMQQGLHEPYNLQK
                     ILLGGAKLSATMIETALQYNLPIYNSFGMTETCSQFLTATPEMLHARPDTVGMPSANV
                     DVKIKNPNKEGHGELMIKGANVMNGYLYPTDLTGTFENGYFNTGDIAEIDHEGYVMIY
                     DRRKDLIISGGENIYPYQIETVAKQFPGISDAVCVGHPDDTWGQVPKLYFVSESDISK
                     AQLIAYLSKHLAKYKVPKHFEKVDTLPYTSTGKLQRNKLYRG"
     gene            complement(7748..8203)
                     /locus_tag="PPY92_001818"
     CDS             complement(7748..8203)
                     /locus_tag="PPY92_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4909 domain-containing protein"
                     /protein_id="HDJ2625808.1"
                     /translation="MKICLMLYLLIFLSSCGTNNKIVGQRFEIQQHNETIGSIYFSAD
                     YAHIRGIEKGTAKYFIDKVGSKRYLFIEYIPDNVLNCKPDFWKTLKYKKDKVTYYVYL
                     IENLDDEVFHLSALQDMNRIPIDIADDVATMGKSPHQNDRMTLKLNKNN"
BASE COUNT         2543 a         1623 c         1307 g         2764 t
ORIGIN      
        1 atttagctat attaaattca tcttaatatt ttactgcgtc ttttaattct tccactttgt
       61 ctaatttttc ccatgggaat aatacatctg tacgtccaaa atgaccataa gcagcagttt
      121 gtttgtaaat cggttgtttc aaatcaagca ttttaataat gcctgcaggt cttaggtcaa
      181 agtgttttct aactgcttcg acaagttgtc cttcagaaac tttacctgtt ccaaatgtat
      241 caattgcaat tgacactggt tctgcaacac caatcgcata tgccaattgt acttcacatt
      301 gatctgctaa acctgctgca acaatatttt tagccacata acgtgcagca tatgcagctg
      361 agcggtctac ttttgtagga tccttaccac tgaagcatcc gccaccatga cgtgcatagc
      421 caccatacgt atcaacaatg attttacgtc ctgttaatcc tgcatcacct tgaggtccac
      481 cgattacaaa gcgtcctgta ggattgatgt agaatttagt ttgttcatta atcaagtttt
      541 ctggaacagt tggataaatg acatgcgctt tgatgtcttc ttgaatttgt tcaagtgtca
      601 catcatcagc atgttgtgtt gatacgacaa tcgtatcaat acgtactggg ttatcatttt
      661 catcatattc aacagtgacc tgaactttac cgtctggtcg taaataattt aacgtaccat
      721 ctttacgcac atcagataaa cgttttgcca attgatgtga taaataaatt gctagaggca
      781 tgtacgtttc tgtttcattc gttgcgtaac caaacattaa accttggtca cctgcacctg
      841 ttgcttcaat ttcttcttcg ctatctttat cacgatactc taatgcttta tccacgcctt
      901 gtgcaatgtc aggtgattgt tcatcaatcg cagttaaaat tgccattgtt tcataatcat
      961 aaccatattt tgctcttgtg tatccaattt ctttaattgt ttctctaaca actttcggaa
     1021 tatcaacata tgttgttgta gaaatttcgc cggcgatcaa tgccatacct gttgtaacag
     1081 ttgtttcaca agctacacgt gcatttgggt cgtcttttaa aatagcatct aatattgcat
     1141 ctgacacttg gtcagcgatt ttatctgggt gtccttctgt aacagactct gaagtaaata
     1201 atcgtttgtt atttaacata gtttgctcct ttaaatttat attacgaaaa ttctctctct
     1261 gtgagctaaa taaaaaagac cttctaacta ttaatataga gagaaggcct aatacgtcca
     1321 ttcgctctta tcgttcagac ctatttgtct gcaaacggtt tggcaccttt cttttataaa
     1381 aaaagaggtt gctgggtttc attgggtcca tgtccctcca ccactcagga taagagaatc
     1441 cgttaaaaat aatagtacct aattaatgaa ttaatgtcaa tttttcacaa ataaatttac
     1501 agtaaaatat tgtagattaa ttatgttaat gtgttatact aattaaatgt aaaggcttac
     1561 atttaaatta tcgctttgga gggatttagg atgtcagtag acacatacac tgaaacaact
     1621 aaaattgaca aattactgaa aaaaccaacg tcacattttc aactttcgac gacacaactt
     1681 tataataaaa tcttagacaa taacgaaggg gtattaacag aacttggtgc tgttaatgca
     1741 agtactggaa aatatactgg tcgttcgcct aaagacaaat tttttgtctc tgaaccttca
     1801 tatagagata acattgattg gggagaaatt aatcaaccta tcgatgaaga aactttcttg
     1861 aagttatacc ataaagtact agactattta gataaaaaag atgaactata cgtatttaaa
     1921 ggctacgctg gtagcgataa agatacaatg ttaaaactta cagtcatcaa tgaattagca
     1981 tggcataatt tatttgctaa aaatatgttt attagacctg aatcaaaaga agaagctaca
     2041 aagattaaac ctaacttcac tatcgtttct gcaccacatt ttaaagcaga tccagaagtt
     2101 gatggtacta aatctgaaac ctttgtcatt atttcattta aacacaaagt cattttaatc
     2161 ggcggtactg aatacgctgg tgaaatgaaa aaaggtatct tctctgtaat gaattatctc
     2221 ttaccgatgc aagatattat gagcatgcat tgctcagcaa acgttggtga aaaaggcgat
     2281 gttgcattat tctttggtct atctggcact ggtaaaacaa ccttatcggc tgacccacac
     2341 cgtaaactaa tcggtgatga tgaacacgga tggaataaaa acggggtctt taatatcgaa
     2401 ggtggctgct atgcaaaagc aattaatctt tccaaagaaa aagaaccaca gatttttgac
     2461 gcaatcaaat atggtgcaat tttagagaac actgtagttg cagaagatgg ttcagtggac
     2521 tttgaagaca atcgttatac agaaaacacg cgtgccgctt atccaattaa tcacattgac
     2581 aatattgtag taccatcaaa agcagcacat ccaaatacaa ttattttctt aactgcggat
     2641 gcatttggtg ttattccacc gatttcaaag ttaaataaag accaagcaat gtatcatttc
     2701 ttgagtggtt tcacttctaa attagctggt acagagcgtg gtgtgacaga acctgaacca
     2761 tcattctcaa catgtttcgg agcaccgttc ttcccgttac accctactgt ttacgctgat
     2821 ttattaggtg aacttatcga tttacatgat gttgatgttt atcttgttaa tactggatgg
     2881 actggcggaa aatatggtgt aggacgtaga atcagcttac attacacacg tcaaatggta
     2941 aaccaagcga tttctggcaa attgaaaaat gcagaatata caaaagatag tacgtttggt
     3001 ttaagcattc ctgtagaaat tgaagatgta ccgaaaacaa ttttaaatcc aattaatgct
     3061 tggagcgaca aagagaaata taaagcacaa gcagaagatt taattcaacg ttttgaaaag
     3121 aacttcgaaa aatttggtga aaaagttgaa catattgctg aaaaaggtag cttcaacaaa
     3181 taaatttgaa tactaattaa agagccaacc gttgttatag tctaacaatg gttggctcct
     3241 cttattttat gtgctaaaaa tttataggca attttattac aacaatgcac atttaaggtg
     3301 accttcatgc caaactcgca tcactcattt aatggaagca gcacgtcttc acataaagta
     3361 ccggttcctt acgctatgca tgttgcccca ctttgcataa agtacttatc gttaactcaa
     3421 cgcaagttgt tcctattcac aaaaagcaca tacagttatc tgcatggagc acattttcat
     3481 tgaaagcgca tacccctcat tcaacgtata tcacacatct tcaaagcttg atagttccca
     3541 tgcgcacacc acgtttcata ctagctatgc aactcaactt gtttcataaa ctctttaata
     3601 taagtcaatg tttcaaccat cgctggtggt cttggcacat gtccttctgc catttgataa
     3661 aatgtttcat gcgtggcacc ttttaactct agttggtccg ctaaataata cgcatgatga
     3721 ataccaactt gctggtcttt ccctccatgt acaattaata ttggcggact gttttcatta
     3781 atgtttggaa tcgcttggcg tgcctcatat gccgctcgat cttttttcgg atgaccaatc
     3841 attcttcgta gcatgcctct taaatcgaca cgttcttcat acattaaatc aatatctgag
     3901 acaccacccc agattgtata acttgttact ggtaagtctt gaaatgtcaa caatccttgt
     3961 aaaccacctc gcgaaaaacc aaccatgtgg ataaatgcat gtggatattt atcatgtagc
     4021 aaccttaata attgcgtcac atcatttaaa tcgccacggt aaaattcgtc tttaccttca
     4081 ctcccattgt tacctcggta gtatggccca atcactaaag tttgactatc tgaaaattgc
     4141 attaatctac ctgcgcgcac acgtcctact tgacctttgc cacctcgcaa ataaactaca
     4201 atgcgattta cttcatgatg tggtgtcatc attaaagctt ttacttgtaa gtcatctgac
     4261 aaatatgtaa tttcttcgaa ttgatgcgta aaagattcaa ttggcattcg tttacgtttg
     4321 ataaaatcca agtgattgca ccctctctac gcattttaaa atggtactat cttgcagtaa
     4381 gaaactccgt tgtgcgagtt caatatcatt gatacagtta aacaacactg gccctgctgt
     4441 ttctaaataa tcgttcttgc ttaccaatga ttcaacttcg ataaaatata catcttttac
     4501 aaaatcagtt tgatcatgtg tttcaatggt atattgtgct atgtaatgaa tatttttaac
     4561 tttggcgcct gtttcttcat ataattcacg ggtaactgct tcagcactac tttccccgcg
     4621 ttccctttta ccaccaggaa attcaatccc ccgtaaatta tgtttggtaa aaagcaattg
     4681 atttttaaac gttggaatag ctagcacatg attgccatct gctatctcat tatccttttt
     4741 aaatgtcaaa ttaacttgac gattatcttt atccctaaac ttcacgcgca tcacatccct
     4801 acattgtatg ttaatataat agttaattac tatcgttgga ggcattaatt atgaaaaaga
     4861 tattcttggc gatgattcat ttttatcaac gtttcatttc gccactcact ccaccaactt
     4921 gtcgttttta tccaacatgt tcagagtaca ctagagaagc gattcaatac cacggtgctt
     4981 tcaaaggcct ttatttaggc atccgtcgta ttttaaaatg tcatccgctt cataaaggtg
     5041 gctttgaccc tgttccgtta aaaaaagaca agtcagcaag caagcattca cataaacata
     5101 accattaata tggttgtaat tgagttatat ccactaaagg ggggcgaaat tcgagtcgcc
     5161 cctcttttaa tatgcctgat ttagtaacca catcttgatc aaaataataa cctgctggtg
     5221 taacatctcc tggataatca cctttacgag caagcatcgc tgtaaaataa cggcttaaac
     5281 catattcgta catgccgcca ataaccactt ttacaccatg acttttcaaa gtatcaattg
     5341 ccgtttgcac tttatcaatg ccacctagac gaaatggttt taatacaaca actttcacat
     5401 tgtataattc tatcaaatta attatgtcca acaacgatgt tgccttttca tcaagggcta
     5461 ttggaggtat tgttccatcc actacttcat caagcatgga gatatcttta aatggctctt
     5521 cgatataaag aacctgttca cgcgctaata gctgtaactg tgtgaaatct tgacgatcca
     5581 atgactcatt tgcatctata accaattgaa agtgaaagtc taattcccgt aacactctaa
     5641 tttgatgcat gatttgaggc gtccatttta atttaattct ggtcggcttt gttgctttca
     5701 atgactctag ttgtttattt gataagccgc tcgctgtcgc tccatatgct actgaaaatg
     5761 aaggcagtac atgaaacatt tgatacaatg ccatgacaat agttgccctt gcagcaggcg
     5821 tattttccaa tgaatctact aattttagtg ctgcttcata cgtttcaaat gatttatttc
     5881 tattatcttc gaaccattgc tcaattacat gtttcactga agcaattgtt tcatgatcat
     5941 accaatctgt ttgaaaagcg ttacattccc cgaaatatgc atttcctttg tcatcaatca
     6001 attcgataaa caaacaatca cgatgcgtta aagtgacttt cggtgttaca atttgtgact
     6061 taaatggctc actatattta taaaaatgca aagctgtcaa cttcatcaaa tcatcctcta
     6121 tacaacttat ttctctgtaa tttacccgtt gatgtataag gtaaagtatc aaccttttca
     6181 aagtgtttcg gtactttata tttcgctaaa tgttttgata aatatgcaat caattgtgcc
     6241 tttgaaatgt cactttcact gacaaaatat aatttaggca cttggcccca agtatcatca
     6301 ggatgcccta cacatactgc gtcactgata cctggaaatt gcttcgctac cgtttcaatt
     6361 tgatatggat aaatattttc accgccacta ataattaaat ctttacgtcg gtcataaatc
     6421 ataacataac cttcatgatc tatttcagca atgtcacctg tattaaaata accattttca
     6481 aacgtacccg ttaaatctgt tggatacaaa tatccattca tcacattggc acctttaatc
     6541 attaattctc catgaccttc tttattagga tttttaattt taacgtcaac attggcactt
     6601 ggcatcccta cagtgtcagg acgtgcatgc aacatttccg gtgttgctgt taaaaattgc
     6661 gaacatgtct cagtcatacc aaatgaatta taaattggca ggttatattg taatgccgtc
     6721 tctatcatag tggcagataa tttagcaccg ccgagtaata ttttttgtaa attataaggt
     6781 tcatgtaaac cttgttgcat aagccaattt aaagtttgtg gcacaagcga aatgtgcgtg
     6841 attcgttcat ttttaatcat cgttaaaatt tgttcggcat tgaatttatc aacaatgcgc
     6901 acagtaaaac cttcaataac agctcttaaa agtacactga gacccgaaat atgataaatc
     6961 ggcaagacag atagccaatt agtgtcacga tcaaatccca agctctcttt acatccgatt
     7021 gcactggcat aatgattacg aaacgtttgt ggcaccgctt tttgagggcc cgttgtccct
     7081 gatgtaaaca taattgatgc aatgtcatct aaattaaatg atgtatttaa tatgttggac
     7141 ggcgactctt tcggcaccac agtttcattc gatgtatcat attggatacc cattgtgttg
     7201 tccaacaaac cgttcgttgt aatatccctt ccagcgaatt caatatcatc cagcgataca
     7261 atttgaaacc ctcgcaattc cagtggcaag gtacaaaaaa tcaattgtac atcgattgac
     7321 ctcatctgat tcgtcatctc attaggtgtc aaccttgtat taatcatcgc aatttcaata
     7381 tttgccaacc aacatgcatg tattaaaatg atcgattgaa tcgaattatc tatgtatagc
     7441 ccgacacgag attgttgata agccttgagt cttttagcca atagactcgc ttcacagtat
     7501 aaattttgat aagtataaga ttcttgaccg tctgttatcg caatatgatg tccattttgt
     7561 tgtgcttgtt tatataacca aaagtccatg cgttattcct ccaaaatcat ttacattata
     7621 attataacga ttttatgaca ttctagcagt ggttatgttt aaaaatataa aaaagtagac
     7681 gaattgattc attgatatga ttgttataat gctcaataca tatcgttata tcattcgtct
     7741 actattatca gttattttta tttaatttta gtgtcattct gtcattttga tgtggtgatt
     7801 tacccattgt tgccacatca tctgcaatgt caattggtat acgattcatg tcttgtaatg
     7861 cacttaaatg gaatacttca tcatctaaat tttcaatgag atatacataa tatgttacct
     7921 tgtccttttt atattttaac gttttccaaa agtccggctt gcaattcaat acattatccg
     7981 gaatatattc aataaataag taacgtttgc tgcctacttt gtctatgaaa tattttgcag
     8041 tgcctttttc tatacctctt atatgtgcat agtctgctga aaagtaaata ctacctattg
     8101 tttcattatg ttgttgtatt tcaaatcgtt ggcctactat tttattattt gtgccacaac
     8161 tacttaaaaa aatcagtaga tatagcatta aacatatttt catccccttg aattttaaaa
     8221 actttttcaa aagcaac
//
LOCUS       DANBWH010000119         8205 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.122, whole genome shotgun sequence.
ACCESSION   DANBWH010000119 DANBWH010000000
VERSION     DANBWH010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8205
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.122"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            283..864
                     /locus_tag="PPY92_001819"
     CDS             283..864
                     /locus_tag="PPY92_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDJ2625809.1"
                     /translation="MTIYLVRHGESKSNYDNKHFRSYFCGQLDVPLTDTGTKSADDLC
                     DYFKEKQIKHVYVSDLLRTQQTFEHIFPYDIASTTTPLLRERSLGVFEGEYKDEISAN
                     PKYEKYFNDPNFKDFRHSFSQKAPEGESYEDVYQRVEHFMNHVVNEDTQKDDIVIVAH
                     QVVIRCLMVYFNKVSREEAVDLKVENCKPYIIE"
     gene            complement(930..1313)
                     /locus_tag="PPY92_001820"
     CDS             complement(930..1313)
                     /locus_tag="PPY92_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625810.1"
                     /translation="MKVRITFIILAILSTIVCLFLAAMYPTGPNTVTFEQPYLFTLNI
                     ILMVLVALPSLVLAIYDYMSFRISSAILQFLGAISWFFLSLILSLTQFTPFTLASFIT
                     SIILFISAIITLAIGGKSVDKNDSH"
     gene            complement(1583..2209)
                     /locus_tag="PPY92_001821"
     CDS             complement(1583..2209)
                     /locus_tag="PPY92_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDxxF motif lipoprotein"
                     /protein_id="HDJ2625811.1"
                     /translation="MKKRLLLSTFLASALILTGCASDQSDNEDHHTSTGIHAPKSAKK
                     LETKDIFNSDKKNSDISDAEMKQAIEKYLSVNSDILDNKYIMQHKLDKQIDSQTKVTE
                     KQAETLSHLSNLAVKNDLHFKKFVTENNIPKEYKKPVELMMNYFKALNSTIANVDEDI
                     EKLSYQPQNKINVVDVPTKYAGDVNKKQQDKIKDFLKSKGIKSDVIDK"
     gene            complement(2282..2500)
                     /locus_tag="PPY92_001822"
                     /pseudo
     CDS             complement(2282..2500)
                     /locus_tag="PPY92_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2653..4176)
                     /gene="ahpF"
                     /locus_tag="PPY92_001823"
     CDS             complement(2653..4176)
                     /gene="ahpF"
                     /locus_tag="PPY92_001823"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDJ2625812.1"
                     /translation="MLNADLKQQLKQLLELMEGNVEFVASLGSDEKSKELKELLTEIS
                     DMSPRLSLSEKSLKRTPSFSVNRPGEETGVTFAGIPLGHEFNSLVLAILQVSGRAPKE
                     KQSIIDQIKNLEGSFHFETFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREE
                     SENIMAVPAVFLNGEEFGNGRMTIQDIFSKLGSTADASEFENKEPYDVLIVGGGPASG
                     SAAIYTARKGLRTGIVADRIGGQVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDID
                     AMTGIRATDIEKTDEAIKVTLENGAVLESKTVIIATGAGWRKLNIPGEEQLINKGVAF
                     CPHCDGPLFENKDVAVIGGGNSGVEAAIDLAGIVNHVTLFEFASELKADNVLQDRLRS
                     LSNVDIKTNAKTTEVVGEDHVTGIRYEDMSTGEEHLLNLDGIFVQIGLLPNTSWLKDA
                     VELNERGEIVIDRNNNTNVPGIFAAGDVTDQKNKQIIISMGAGANAALNAFDYIIRN"
     gene            complement(4192..4761)
                     /gene="ahpC"
                     /locus_tag="PPY92_001824"
     CDS             complement(4192..4761)
                     /gene="ahpC"
                     /locus_tag="PPY92_001824"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDJ2625813.1"
                     /translation="MSLINKEILPFTAQAFDPKKDQFKEVTQEDLKGSWSVVCFYPAD
                     FSFVCPTELEDLQNQYEELQKLGVNVFSVSTDTHFVHKAWHDHSDAISKITYTMIGDP
                     SQTITRNFDVLDEATGLAQRGTFIIDPDGVVQASEINADGIGRDASTLAHKIKAAQYV
                     RKNPGEVCPAKWEEGAKTLQPGLDLVGKI"
     gene            5253..6008
                     /locus_tag="PPY92_001825"
     CDS             5253..6008
                     /locus_tag="PPY92_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HDJ2625814.1"
                     /translation="MSDYVYNLVKKHHSVRKFKNKPLSEDVVKKLVEAGQSASTSSFL
                     QAYSIIGIDDEKIKENLREVSGQPYVVENGYLFVFVIDYYRHHLVDQHAETDMENAYG
                     STEGLLVGAIDAALVAENIAVTAEDMGYGIVFLGSLRNDVERVREILDLPDYVFPVFG
                     MAVGEPADDENGAAKPRLPFDHVFHHNKYHADKETQYAQMADYDQTISEYYDQRTNGN
                     RKETWSQQIEMFLGNKARLDMLEQLQKSGLIQR"
     gene            complement(6088..7476)
                     /locus_tag="PPY92_001826"
     CDS             complement(6088..7476)
                     /locus_tag="PPY92_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDJ2625815.1"
                     /translation="MNAFLTLINIIVLVIFIVILHMMARKHISFAKRVFTALGIGIVF
                     GVLLHLVYGTHSNVITSTSDWFNIVGQGYVALLQMIVMPLIFISIVAAFTKIQIGEKF
                     AKIGSLIFIFLIGTVTIAAIVGVVYALVFGLDASTINLGNAEQARGSEIAKQAKDLTA
                     HTLPQQILELLPKNPFLDFTGQRATSTIAVVIFASFIGFAYLRVARKQPDHGELLKRA
                     IDAIYSLVMAIVTFVLRLTPYGVLAIMANTLSTSDFGAIWTLGKFLIASYAALITMYI
                     IHLIILSLLGISPIRYVKKTLEVLIFAFTSRSSAGALPLNVQTQTRRLGVPEGIANFA
                     ATFGLSIGQNGCAGIYPAMLAIMVAPVANVEIDLQFIVTLIAVVIISSFGVAGVGGGA
                     TFASILVLSTLNLPVALAGVLISVEPLIDMGRTALNVNDSMLAGTGTAKLTKHWDKDT
                     FESNDNAALTSH"
     gene            7561..7659
                     /locus_tag="PPY92_001827"
     CDS             7561..7659
                     /locus_tag="PPY92_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625816.1"
                     /translation="MQLNVNMMKFDRWHKGICKQIVLNLVEKGEKG"
BASE COUNT         2731 a         1315 c         1513 g         2646 t
ORIGIN      
        1 ttttggaaat aaaaaagcga gtatttgaca aataaaaaaa ggatgacgtt gggttgcgcc
       61 atccaggagt attatgttca tgaatataaa agactgtgta gtcatttaag aactacaccc
      121 atatagtaac acatcacttg attaaagaca atactatttt tagagtcatt aatttaaaat
      181 atgaattaat tcttttttta gaatagaata ttaagattga tatcgaatta gtagtcaaag
      241 tgttatggta gatatgaaat acataaggta aggagtaata ttatgacgat ttatttagtt
      301 agacatggcg aatcaaaatc gaattatgat aataaacatt ttcgatctta tttttgtgga
      361 caattagatg tgccgttaac ggatactggc acaaaaagtg cagacgattt atgtgattat
      421 tttaaagaga aacagattaa acatgtatat gtttcagact tattaagaac acagcaaacg
      481 tttgaacata tttttccata tgacattgca tcaacgacta cccctctatt aagagaacgt
      541 tcacttggcg tatttgaggg tgaatataaa gatgaaatca gtgcgaatcc gaaatatgaa
      601 aaatatttca atgatccaaa ctttaaagac tttcgtcata gtttttcaca aaaagcgcct
      661 gaaggagaaa gttatgaaga tgtatatcaa cgcgtagaac attttatgaa tcatgttgtc
      721 aatgaagata cacaaaaaga tgatattgtc attgttgcac atcaagttgt cattcgttgt
      781 ttgatggttt attttaacaa agtttcaagg gaagaagctg tggatttaaa ggtagaaaat
      841 tgcaaaccat atatcattga atagaagtat agaggttctg aaaagctgtg ttttatagtg
      901 gccttcagaa cctttttggc atttggaatt taatgggaat cattcttatc aacagactta
      961 ccaccaatag ctaatgtgat aattgcgctt atgaacaaaa taattgaagt tataaatgac
     1021 gctaatgtaa aaggtgtaaa ttgtgtgagc gataatatca atgataaaaa gaaccaagag
     1081 atagccccta gaaattgtaa aatagcagaa gaaattctaa aactcatata atcatatata
     1141 gctaaaacaa gtgaaggtaa tgcgactaat accattaaaa tgatgttgag ggtgaataaa
     1201 tatggctgtt caaaagttac tgtgtttggt cctgttggat acattgctgc taagaacaag
     1261 caaacaatcg tcgataaaat tgctaaaata ataaaagtaa ttcgaacttt catcatgacc
     1321 atcctttgtt tatagagtca atataagtat gaaatatgtt agggatatag tcaaatgcgt
     1381 caactaatgg gtgattttgg catagataga gaatttaagg caattaaaaa agcatcaaac
     1441 agtaatatgc tgcttgatgc aaaatggtaa ggtattggtt aaattgtatt gtctcaaaga
     1501 ttagtcactt ttaaagataa agaattgtca tgaattaaag ctcatgtaat gatgtgttac
     1561 atgtcgcaat gatggctttc agttatttat cgataacatc actcttgata cctttagatt
     1621 ttaagaaatc tttaatttta tcttgttgct ttttattaac atcaccggca tattttgttg
     1681 gcacgtcgac tacattgatt ttattttgcg gttgatagct aagcttttca atatcttcat
     1741 caacattggc gattgtacta tttaaagctt tgaagtaatt catcattaat tcaacgggtt
     1801 tcttatattc tttaggaata ttgttttcag tgacaaattt cttgaaatgc aaatcgtttt
     1861 taacagctaa gttagataag tggctaagtg tttctgcttg tttttcagtc acttttgttt
     1921 gactgtcaat ttgtttatct agtttatgtt gcataatata tttgttatca agtatatcgc
     1981 tatttacaga caaatacttt tctatagctt gcttcatctc tgcatcacta atatcactat
     2041 ttttcttatc tgagttaaag atatcttttg tttctaattt tttagcgctt ttaggtgcat
     2101 ggatgccagt acttgtgtga tgatcttcgt tatcagattg atcggacgcg caacctgtaa
     2161 gaattaatgc agatgctaaa aatgtactta gtagtaatct ctttttcata atgtaatata
     2221 actccttagt ttatctttaa ttgaaaaaat atgtattcat gtttaataga gtaacattga
     2281 attagtttgg aatgtcacga tgaccttgca atgaccatag acgcaaatga ttacgtgcat
     2341 gagttgcttt ttctatcaat aatgcgtcgt tttgaacaat gttaaggata gcattatcta
     2401 taaataactg cataattggt tgtatcaatt tagatgtagg tatcgtacgt aaaagcataa
     2461 tgatttcgtt cacatacttt tccttctcaa tatcattttc atattgattt gtttgcgaga
     2521 ggtacatact ttaagcatta tcgcacatct cgttgtatat attaagttta tcataacatg
     2581 attttatgtc gggataaaaa aataacagca tcttaacaaa tgtaagatac tgtcagtgaa
     2641 actgtgaaaa ctttagtttc tgataatata gtcaaaggca tttaatgctg catttgcacc
     2701 agcgcccatt gaaatgataa tttgtttgtt cttctgatct gtgacatcgc cagcagcaaa
     2761 tattccaggt acgttagtat tattgttacg atcaataaca atttcaccac gttcgtttaa
     2821 ttcaacagca tcttttaacc atgatgtgtt tggaagtaaa ccaatttgaa caaagatacc
     2881 atctaagtta agtagatgtt cttcgccggt gctcatgtct tcgtaacgta tacctgtaac
     2941 atggtcttct ccgacaactt cagtagtttt ggcatttgtt ttgatatcaa catttgataa
     3001 agaacgtaaa cgatcttgta acacgttgtc tgcttttaat tcgctagcga attcgaataa
     3061 tgtaacatga ttaacgatac cagcaaggtc aattgctgct tcaaccccag agttaccgcc
     3121 accgataact gctacgtctt tattttcgaa tagaggtccg tcacagtgag ggcagaatgc
     3181 aacaccttta ttaatcaatt gctcttcacc tggaatgttt agcttacgcc aacctgcacc
     3241 agtagcaata atgactgttt tactttctaa gacagcaccg ttttctaacg taactttaat
     3301 tgcttcgtca gtcttttcga tatctgtagc acgtatacct gtcattgcat caatgtcata
     3361 ttgatcaatg tgcgctgcta agttagaaga aaattcagaa ccagttgttt ctttaacagt
     3421 aatgaagttc tcaataccag cagtatcatt aacttggcca ccgatacgat cagcaactat
     3481 accagtacgt aaacctttac gtgctgtgta aatcgctgca ctaccactag caggaccacc
     3541 accaacgatt aagacatcat aaggttcttt attttcaaac tcagatgcat ctgccgtact
     3601 gcctagtttc gaaaaaatat cttggattgt catacgacca ttgccaaatt cttcgccgtt
     3661 taaaaagaca gcagggactg ccatgatgtt ttcagattct tcacggaaca ctgcaccatc
     3721 aatcatagaa tgcgtgatgt tagggttgat cacactcatt aagttaagtg cttgaacgac
     3781 atcaggacat ttttgacacg ttaaactaat gaatgtttca aaatggaatg aaccttctaa
     3841 gtttttaatt tggtcaataa ttgactgttt ttctttaggt gcacgaccac taacctgtaa
     3901 aattgctaaa acaagtgagt taaactcgtg acctaatgga attcctgcaa atgttacacc
     3961 tgtttcttcg ccaggacggt tgactgagaa acttggtgta cgttttaaag acttttcaga
     4021 aagtgaaagt ctaggtgaca tatcagaaat ttctgttaat aattctttga gttccttaga
     4081 tttttcatct gaaccaaggc tggcaacgaa ttcaacgttg ccctccatta gttctaataa
     4141 ttgtttaagt tgttgtttta aatcagcatt aagcatggtt gtaatgcctc cttagatttt
     4201 acctactaaa tctaaaccag gttgcaatgt tttagcgcct tcttcccatt tagctgggca
     4261 tacttcgcca gggtttttac gaacatattg agctgctttg attttgtgag ctaatgtact
     4321 agcgtcacgg ccaattccgt cagcgttaat ttcagatgct tgtacaacac cgtctgggtc
     4381 gataatgaat gtaccacgtt gagctaaacc agtagcttca tctaatacat caaaattacg
     4441 agtgattgtt tgtgatgggt caccaatcat agtgtaagtg attttgctaa ttgcatctga
     4501 atggtcatgc catgctttgt gtacgaagtg agtatcagtt gatactgaga atacatttac
     4561 gcctaatttt tgtaattctt catattggtt ttgtaagtct tctaattcag ttggacaaac
     4621 gaatgagaag tcagcaggat agaagcatac tacgctccaa gaacctttta aatcttcttg
     4681 tgtaacttct ttaaattgat ctttttttgg atcgaaagct tgcgctgtaa atggtaagat
     4741 ttctttgtta attaatgaca taaatatctt cctcctaaga atttaagtat gaattagaac
     4801 tatcaattga ttgtgcttaa ttataataat tctaatctct tagttagcat tattacattt
     4861 tgatccagaa tagtcaactg gataactttg taaagtgaac gattactttt aaaataaaga
     4921 aagataatat aaagtgcttt gataatggat tttgtagttg atgatttaaa aggttgtgtc
     4981 tatatttaat atcttgattt taatgtaaaa aatgtaaaaa aagaagattt gtattctcaa
     5041 ctaagtcaac cttattgata atggtgtgag aatatttgtt cgagatggat gaaggtgatt
     5101 agtgagaaac tggaattttc aagtacgaga caatattttt aaaagttcaa ttattaactt
     5161 ataagcaaat aattgctata aaaaagtttg gacgtgtaca attgcaatat gaagattttt
     5221 aattaactgt aaagtatcga ggagtgggta acgtgtcaga ttatgtatat aatcttgtga
     5281 aaaagcatca ttctgttaga aaatttaaga ataaaccttt aagtgaagac gttgttaaga
     5341 aattggtaga agctggacaa agcgcttcga cgtcaagttt cctacaagca tactcaatta
     5401 ttggtatcga cgatgagaag attaaagaaa atttacgaga agtttctgga caaccttatg
     5461 ttgtagaaaa tggctattta ttcgtctttg ttattgatta ttatcgtcat catttagttg
     5521 atcaacatgc tgaaactgat atggaaaatg catatggttc aacggaaggt ttgctagtag
     5581 gtgcaatcga tgcagcatta gttgctgaaa atattgcggt aactgctgaa gatatggggt
     5641 atggcattgt ctttttagga tcattaagaa atgatgttga acgagttcga gaaattttag
     5701 acttacctga ctatgtcttc ccggtatttg gtatggcagt aggggaaccc gcagatgatg
     5761 aaaatggtgc agccaagcca cgcttaccat ttgaccatgt cttccatcat aataagtatc
     5821 atgctgataa ggaaacacag tatgcacaaa tggcagatta cgaccagaca atcagcgagt
     5881 actatgatca acgtacaaac gggaatcgca aagaaacatg gtcgcagcaa attgagatgt
     5941 tcctaggaaa caaagcaaga ttagatatgt tagaacaatt gcaaaaatca ggcttaatac
     6001 agcgatagca agataccaaa ataacccgcc ccctctagct taaaatgata agtatagcta
     6061 gagggggcgg gtatttcttg caatgaatta gtgtgaagtt aatgcagcat tatcatttga
     6121 ttcgaaagta tctttgtccc aatgttttgt taacttggcg gtacctgtac cagctagcat
     6181 tgaatcgttc acgtttaatg ctgttctacc catgtcaatc aatggttcaa cggagatgag
     6241 cacgccggct aaagcgactg gcaagtttaa cgttgacaac accaatatgg atgcaaatgt
     6301 agccccgcca ccgacgccag caacgccgaa tgaactaata atcacgacag cgattaacgt
     6361 tacaataaat tgtaaatcaa tttctacatt agcgacgggt gcgaccataa ttgcaagcat
     6421 ggcagggtaa atgcctgcac aaccattttg tccaatcgac aatccaaatg tcgcagcgaa
     6481 attggcaata ccttctggca cgcctagacg tcttgtttgt gtttgtacat tcaatggtaa
     6541 ggcacccgcg cttgagcgtg atgtgaatgc aaagattaat acttccaaag tctttttaac
     6601 atagcgaatt gggctaatac ctaacaggct taaaataatt aagtgaatga tatacatcgt
     6661 aattaatgca gcgtacgatg cgattaagaa ttttcctaaa gtccaaatgg cgccaaagtc
     6721 acttgtcgat aatgtgttgg ccataattgc taatacaccg tatggcgtta aacgtaagac
     6781 gaacgtcaca atcgccatta ctagtgaata gatagcgtca atcgcacgct taagcaattc
     6841 accatgatca ggttgtttgc gtgctacgcg taaataagca aatcctataa acgaagcaaa
     6901 tatcacgaca gcaatcgtgg aagttgcacg ttgtccagtg aaatctaaga atggattttt
     6961 aggcaataat tccaaaattt gttgtggtaa cgtatgtgct gttaaatctt tcgcttgttt
     7021 agcaatttcg cttccacgtg cttgttcagc gttaccaaga ttaattgttg atgcatctaa
     7081 accaaacacc aaggcataca caacaccaac aattgcagca atggtgacag tgccaattaa
     7141 aaagataaaa atgagactac caattttagc aaacttttct ccgatttgaa ttttagtgaa
     7201 tgcagcgaca atagaaatga aaattaaagg catcacaatc atttgcaaca atgcaacgta
     7261 accttgtccg acaatgttga accagtcact tgttgatgta ataacattcg aatgtgttcc
     7321 atagacaaga tgtaataaca cgccaaatac aatgccgata cctaaagctg taaacacacg
     7381 tttcgcaaaa gatatatgtt tgcgagccat catgtgcaat attacgatga aaatcaccaa
     7441 tacaataata ttaatcagtg taagaaaagc attcatgaac gtcactcctc aaatttttga
     7501 atataattcc gactagtatg ctaagaataa tattaatttt aaaatatttc aagagggcat
     7561 ttgcaattaa atgtaaacat gatgaaattc gaccgttggc ataagggaat ttgtaaacaa
     7621 atcgttttga atttagtaga gaaaggtgaa aagggttaat agtgttatga cctagtcgat
     7681 taaagttgat atttgaaaat gaagcagggc attaaatgca ataaattaaa taagttgtca
     7741 ttacagcgtt aataatagaa atgattttaa caggaaaaaa gtgatgaata tttggaaaag
     7801 atatatatcg tgcactgtca taagagataa gatatgagaa aacaaatgca tccttgaatc
     7861 gagatgtggc tatgacggtt tgataaaaat tgctaacctc atatttattg aataaaaaga
     7921 taataaatga tcaaccttga aaagacgctt cgaacaaaca cgttgtaaaa aattctgtcg
     7981 aatcaaaatt agcatctttt gagggttcgt gtgtgcaggt ggtagttcaa aagctaccgt
     8041 aaacaaatat ttttgtttga gaaaaaaggc gtttgtagta aatgtgaata tgagtgtgcc
     8101 cttgtgtgat tcgtcattta ctaacacagc caaacattta aattgaatgg gcttattaag
     8161 atgaatctat ttaggaatac acaggagcca tcccatttaa aaggg
//
LOCUS       DANBWH010000120         8176 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.123, whole genome shotgun sequence.
ACCESSION   DANBWH010000120 DANBWH010000000
VERSION     DANBWH010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8176
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.123"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(20..259)
                     /locus_tag="PPY92_001828"
     CDS             complement(20..259)
                     /locus_tag="PPY92_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HDJ2625817.1"
                     /translation="MSNSDLNIERINELAKKKKEVGLTQEEAKEQTALRKAYLESFRK
                     GFKQQIENTKVIDPEGNDVTPEKIKEIQQKRDNKN"
     gene            complement(396..629)
                     /locus_tag="PPY92_001829"
     CDS             complement(396..629)
                     /locus_tag="PPY92_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625818.1"
                     /translation="MFYNKYKNVSTYIIIFLVSSAAFAIFLLSANVSAHSEQVYEMTD
                     HQIKNNTINKAYEHKDPTNNGEQRDGKVFALIN"
     gene            772..1395
                     /gene="lexA"
                     /locus_tag="PPY92_001830"
     CDS             772..1395
                     /gene="lexA"
                     /locus_tag="PPY92_001830"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDJ2625819.1"
                     /translation="MRELTKRQSEIYNYIKQVVQMKGYPPSVREIGEAVGLASSSTVH
                     GHLSRLEEKGYIRRDPTKPRAIEIVSDQTNDNINMEETIHVPVIGKVTAGVPITAVEN
                     IEEYFPLPEHLTSTHNSDIFILNVVGDSMIEAGILDGDKVIVRSQTIAENGDIIVAMT
                     EEDEATVKRFYKEKNRYRLQPENSTMEPIYLDNVAVIGKVIGLYREM"
     gene            complement(1775..2791)
                     /locus_tag="PPY92_001831"
     CDS             complement(1775..2791)
                     /locus_tag="PPY92_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HDJ2625820.1"
                     /translation="MKKFIVTVVAFLSIIIIAPITEFKPFIHLQNEVRQYIDIHINKE
                     TISAENKLDTPKKQQFAFNNIQMNMSKSDVEKTLNKPKRVTFNEYGTKWYTYYDDDYN
                     NFIMISYMKDKVNALYTNQNIITSKSKIKYNTPKSVVRQRLGEPETEIVKGRVRYEQN
                     NKEYDVFHKNHIYTTVFYDKHRRNNVTAVLQVSDAMENRLKEQYGAPSKSLADSFELQ
                     NFDLVNAERKQHQLSTLKYSKQNSETARKHSKDMAKNHYFDHTNLKGQSPFDRLKKDG
                     ITFNSAGENLAYGQVSSIYAHQGLMNSIGHRKNILNDTFKILGVGVDFNDEKQPFWTE
                     NYTG"
     gene            complement(2813..3790)
                     /gene="guaC"
                     /locus_tag="PPY92_001832"
     CDS             complement(2813..3790)
                     /gene="guaC"
                     /locus_tag="PPY92_001832"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDJ2625821.1"
                     /translation="MKIFDYEDIQLIPNKCIVESRSECDTTIQFGPKKFKLPVVPANM
                     QTVMNEKLAKWFAENDYFYIMHRFDEEARIPFIKHMQNSGLFASISVGVKKAEFDFIE
                     KLAQEKLIPEYITIDIAHGHSDSVINMIKHIKNHIPDSYVIAGNVGTPEGVRELENAG
                     ADATKVGIGPGRVCITKIKTGFGTGGWQLAALNICSKAARKPLIADGGIRTHGDIAKS
                     IRFGASMVMIGSLFAAHEESPGETVELDGKQYKEYFGSASEFQKGEHKNVEGKKMFVE
                     HKGSLMDTLKEMQQDLQSSISYAGGKDLKSLRTVDYVIVRNSIFNGDRD"
     gene            complement(3947..4216)
                     /gene="rpsN"
                     /locus_tag="PPY92_001833"
     CDS             complement(3947..4216)
                     /gene="rpsN"
                     /locus_tag="PPY92_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDJ2625822.1"
                     /translation="MAKKSKIAKERKREELVNKYYELRKELKAKGDYEALRKLPRDSS
                     PTRLTRRCKVTGRPRGVLRKFEMSRIAFREHAHKGQIPGVKKSSW"
     gene            complement(4670..4819)
                     /gene="rpmG"
                     /locus_tag="PPY92_001834"
     CDS             complement(4670..4819)
                     /gene="rpmG"
                     /locus_tag="PPY92_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDJ2625823.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERIEMKKYCPRLNKYTL
                     HRETK"
     gene            complement(4910..6427)
                     /locus_tag="PPY92_001835"
     CDS             complement(4910..6427)
                     /locus_tag="PPY92_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HDJ2625824.1"
                     /translation="MSQQDKKLTGVFGHPVSDRENSMTAGPRGPLLMQDIYFLEQMSQ
                     FDREVIPERRMHAKGSGAFGTFTVTKDITKYTNAKIFSEIGKQTEMFARFSTVAGERG
                     AADAERDIRGFALKFYTEEGNWDLVGNNTPVFFFRDPKLFVSLNRAVKRDPRTNMRDA
                     QNNWDFWTGLPEALHQVTILMSDRGIPKDLRHMHGFGSHTYSMYNDSGERVWVKFHFR
                     TQQGIENLTDEEAAEIIATDRDSSQRDLFEAIEKGDYPKWTMYIQVMTEEQAKNHKDN
                     PFDLTKVWYHDEYPLIEVGEFELNRNPDNYFMDVEQAAFAPTNIIPGLDFSPDKMLQG
                     RLFSYGDAQRYRLGVNHWQIPVNQPKGVGIENICPFSRDGQMRVVDNNQGGGTHYYPN
                     NHGKFDSQPEYKKPPFPTDGYGYEYNQRQDDDNYFEQPGKLFRLQSEDAKERIFTNTA
                     NAMEGVTDDVKRRHIRHCYKADPEYGKGVAKALGIDINSIDLETENDETYENFEK"
     gene            6631..8085
                     /locus_tag="PPY92_001836"
     CDS             6631..8085
                     /locus_tag="PPY92_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDJ2625825.1"
                     /translation="MEDNKMNRSLNSRHISMIAIGGAIGTGLFVATGNIISQAGPGGA
                     ILAYLVIGVMLYFLMSSIGELATFYPVSGSFSSYSTRFIDSSLGFTMGWLYWALWSLV
                     TSVDVIVASNVLYFWDTFKFFHPITWSLIFITILLLLNIFSVKSFGETEFWLSLIKVL
                     TIIVFVIFGFLMIFGILGGHTYGFENYTKGQAPFVGGISGFLGVLLVAGFSVGGTEVV
                     AVTAGESDDPKKSMPKAIKQVFWRILLFYVLSIAVIGAIIPYTDPSLLRASSSISQSP
                     FTIVFDRVGIAFAASVINAVILTSLLSAANSGVYTTGRMLYSLSSDKKAPQFLSKLNK
                     TTKLPLRALLTTYAVVVIVIIYANFNSNAVFNLLEIIGSMIIVVWGSSIWSQIRLRQA
                     IKKQGQDPNKVLPYKAPFYPLGPIIVITTLLFLLFGGSVEYILKDQWLNAFKNFLPLI
                     ILALIYFIHKIIHKTKFVKLETINLKPHDYDNQK"
BASE COUNT         2550 a         1433 c         1157 g         3036 t
ORIGIN      
        1 ttctttacag atttgtgatt taatttttat tatctctttt ttgttgtatc tctttaattt
       61 tttcaggtgt tacatcatta ccttctggat caattacttt agtattttca atttgttgtt
      121 taaacccttt tctaaaactc tcaagataag cttttcttaa ggctgtttgc tcctttgctt
      181 cttcttgagt taatcctact tcttttttct ttttagctaa ctcgttaatt ctttcgatat
      241 tcaaatcaga attactcaat taaaaaacct accttcaatt tgtttattca atcattataa
      301 tactttaaca aaatataaca aatttaagac cagcaaaaaa gtaataaaaa aacgctattt
      361 cgcaaaaata ggcaaaataa cgttgtgaca atgtatcaat ttattaaagc gaacactttc
      421 ccatctcttt gttcgccatt gtttgtaggg tctttatgtt cgtatgcttt atttatcgta
      481 ttgttcttaa tttgatggtc agtcatttcg tacacttgtt ccgagtgagc actaacgttc
      541 gcacttaaca agaatattgc aaaggctgca cttgaaacta aaaatatgat gatatatgtt
      601 gatacgtttt tatatttatt gtaaaacatc atttccactc ctagaacatt tgtttgtata
      661 agtaatttat cagaacttat gttctgggtc aatattttaa cgaacaaatg tttgttttgg
      721 ttgtgaatta atgatatact gtatttaaat taatttaggg agtgcctata tatgagagaa
      781 ttaacaaaac gacaaagcga aatatataac tatattaaac aagttgttca aatgaaaggt
      841 tatccgccta gtgttcgcga aattggtgaa gcagttggct tagcatccag ttcaactgtt
      901 catggtcatc tttcacgtct tgaagaaaaa ggatatataa gaagagatcc aacgaaacca
      961 cgtgctatag aaattgtaag tgatcaaaca aatgataata ttaatatgga agaaacgatt
     1021 catgtgccag ttattggtaa agtcacagca ggtgttccta ttaccgctgt agaaaatatt
     1081 gaagagtatt ttccattacc tgaacactta acatcgacac ataatagcga catattcata
     1141 ttaaacgtcg taggcgacag tatgattgag gctggtatat tagacggaga caaagtaatt
     1201 gttcgcagtc aaaccatagc agaaaatgga gacattattg ttgctatgac tgaggaagat
     1261 gaagcaactg tcaaacgctt ctataaagaa aaaaatcgtt atcgattaca acctgaaaat
     1321 agtacaatgg agccaattta cctcgacaat gttgctgtaa ttgggaaagt aattggtttg
     1381 taccgcgaaa tgtaatattt taaaccgtta tatattatcg taattgttaa accctcattt
     1441 ttataaattt tggacctctt gaaaaagttt cgttttcaag aggttttatt tattctaatc
     1501 taaattcagt tcaaacagaa attgcgaatt gttagataat ctcattcttt aatataaatt
     1561 ttgaattaca gattcctgca aaatgttaca caaattaaaa ctcgcccccg taataattta
     1621 caggagcgag ccattattat ttttttcttt cttttttatt aagtatatct ttgacgcgtt
     1681 caagtatatc cgcatctttt ggtttatctg ttgtcatcat gtttatctac ttccttacag
     1741 acaaacatta ccctttaaac tcataccaaa aacattaacc agtataattt tctgtccaaa
     1801 aaggttgttt ttcatcatta aaatcaacac caacacctaa tattttaaac gtatcattta
     1861 aaatattttt tctgtgacca atagaattca ttaatccttg atgtgcatag atactactaa
     1921 cttgaccata tgctaaattc tctccggctg agttaaatgt aataccatct tttttcaatc
     1981 gatcaaatgg tgattgacct tttaaatttg tatgatcaaa ataatgattt ttggccatat
     2041 ctttactatg cttacgagca gtttcagaat tctgtttaga atacttcaat gtagataatt
     2101 gatgttgttt tctttcagca ttaactaaat caaaattttg tagttcaaaa ctatctgcaa
     2161 gcgatttcga aggtgctcca tattgttctt ttaatctatt ttccatagca tcacttactt
     2221 gtaaaacagc tgttacatta ttacgtcgat gcttatcata aaataccgtc gtataaatgt
     2281 gatttttatg gaaaacatca tattctttat tattttgttc gtaacgcact ctacctttaa
     2341 caatctctgt ttctggttcg cctaatcttt gccttacaac cgatttaggt gtattgtatt
     2401 taatttttga ttttgaagtg attatatttt gatttgtata taacgcatta actttatctt
     2461 tcatgtaact tatcattata aaattattgt aatcgtcatc ataatacgta taccacttcg
     2521 taccatattc attaaatgtc actctttttg gtttatttaa tgttttctca acatctgatt
     2581 tcgacatgtt catttgtata ttattaaagg caaattgttg tttcttcggt gtatccaatt
     2641 tattttccgc agaaattgtt tctttattga tgtgaatgtc aatatattgt cttacttcat
     2701 tttgtaaatg aatgaatggt ttaaattctg ttattggcgc aatgataata attgataaaa
     2761 acgcaacaac agttactatg aattttttaa tttacatcac ctactataaa tattaatctc
     2821 tatcaccgtt gaaaatagag tttctaacaa taacataatc tacagtacgt aatgatttca
     2881 agtcttttcc accggcatat gaaattgagc tttgtaaatc ttgttgcatt tcttttaagg
     2941 tatccattaa tgaaccctta tgttctacaa acattttttt accttctaca tttttatgtt
     3001 cgcctttttg aaattcagat gcactaccaa aatattcttt atactgttta ccatcaagtt
     3061 ctacagtttc accaggtgat tcttcgtgtg ccgcaaataa tgaaccaatc atgaccattg
     3121 atgcaccaaa tctaattgat ttagcgatgt cgccatgcgt tcttatacca ccatcggcaa
     3181 tcaaaggttt acgagctgct ttactacata tgtttaatgc cgctaactgc caaccaccag
     3241 taccaaaacc tgttttaatc tttgtaatac aaactctacc aggacctata ccgactttgg
     3301 tagcatcagc accagcattt tctaattctc taacaccttc tggcgtacca acattaccag
     3361 caataacata actatcaggt atatggtttt ttatatgttt aatcatgttt atcactgaat
     3421 ctgagtgacc atgcgcaata tctattgtaa tatattcggg gattaatttt tcttgagcta
     3481 acttttcaat aaaatcaaat tccgctttct ttacaccaac tgaaatagat gcaaataagc
     3541 ctgaattttg catatgtttt ataaaaggta ttcttgcttc ttcatcaaaa cgatgcatga
     3601 tataaaagta atcattttca gcaaaccatt tcgctaattt ctcattcata actgtttgca
     3661 tatttgcagg aactacaggt agcttgaatt ttttcggacc aaattggata gttgtatcac
     3721 attcagacct actttcaact atgcatttat taggtattaa ttgaatatct tcgtaatcaa
     3781 atattttcac tttaaatagc ccctttaaaa actaattatt tttaaatgca aattaaaaac
     3841 cgaatgtttc cttattaata tgagaaaaca ttcagtttat agcattcgta tatactgtac
     3901 attgttttat tgatattgta aatgggctaa agtgcgaaat ttgattttac caacttgatt
     3961 ttttaacacc tggaatttgt cctttgtgcg catgttctct aaacgcaata cgagacattt
     4021 caaatttacg taatacacct ctaggtcttc cagttacttt acatcttcta gttaaacgtg
     4081 taggtgatga atctcttggt aattttctta acgcttcgta atcacctttt gcttttaact
     4141 ctttacgtaa ttcgtaatat ttatttacta actcttctct ttttctctct tttgctattt
     4201 tagatttctt agccatatat agttacctca ctttttatca aatcgtaatt attacgattt
     4261 atataataac acaatcctca cataattcaa cagtaaagtt tcatttcttt gttaaatcat
     4321 tttctaaacc atcatattta agattaaagt gtattatcga acatttataa aacataccca
     4381 gaattacttc gtacttatta aattccgctt tgaaatttta aattctacta tattaaatac
     4441 tctttcaagc attcgctaac aaagtgactt tataaaatca gtaatctatt tccttctata
     4501 aatattctca aattttaata aagtaacaac taatacaatc atatattgtt ctaccgatga
     4561 aactcaatcc attaatcact cttcacaaaa atttcataaa aaagcgagct ccccaatata
     4621 ataggtaagc ccgctttatt ttcaaatcgt cgtatttgaa tgataagaat tacttagttt
     4681 cacgatgtaa cgtatatttg tttaatcttg ggcaatattt tttcatttca atacgctcag
     4741 gattattacg tttattttta gtagtgatat agttacgatc gccacattct gtgcatgcta
     4801 atgttacgtt tacgcgcacg ttaaacaccc tctcttgtta ggaatcttta cgatttatta
     4861 tgatatcata aactgctcaa ctacgcaata tagaaactac atatcaaatt tatttttcaa
     4921 agttttcgta tgtttcatca ttttcagttt caagatcaat agaatttata tcaataccta
     4981 atgcttttgc aacaccttta ccatattctg ggtcagcttt gtaacaatga cgaatatgac
     5041 gtcgtttaac atcatccgtt acgccttcca ttgcatttgc tgtatttgta aaaattcttt
     5101 ctttagcgtc ctctgattgt aatctaaaca atttacctgg ttgttcaaaa taattatcat
     5161 catcttgacg ttgattatat tcatagccgt atccatcagt tgggaatggt ggctttttat
     5221 attcaggttg agaatcaaat ttaccatggt tatttggata ataatgtgtt cctccacctt
     5281 ggttattgtc aactacgcgc atttgaccat ctctactaaa aggacaaata ttttcaatac
     5341 caacaccttt aggttggttt acaggaatct gccaatgatt aactcctaat cgatatcttt
     5401 gcgcatcgcc atatgagaat aaacgccctt gcaacatttt gtctggagaa aaatctaatc
     5461 ctggaataat attagttggt gcaaacgcag cttgttcaac atccataaag taattatctg
     5521 gatttctatt taattcaaac tctccaactt caattagagg atactcatcg tgataccata
     5581 cttttgttaa atcaaatgga ttatctttat ggtttttagc ttgttcctca gtcattactt
     5641 gaatatacat tgtccatttt ggataatcac ctttttcaat ggcttcgaat aaatcgcgtt
     5701 gagatgaatc acgatctgta gctataattt cagcagcttc ttcatcagtt aagttttcaa
     5761 taccttgttg cgttctaaaa tggaatttaa cccaaacacg ttcaccagaa tcattataca
     5821 tagagtatgt gtgagaaccg aacccatgca tatgacgtaa atctttagga atccctctat
     5881 ctgacattaa gatcgttact tggtgcaatg cttctggaag acccgtccag aaatcccagt
     5941 tattttgtgc atctctcata tttgttctag gatctcgttt caccgcacga tttaaactaa
     6001 caaataactt tggatctcta aagaagaata ctggtgtgtt attccctact aaatcccagt
     6061 tcccttcttc agtgtagaac tttaacgcaa atcctcgaat gtcacgctcc gcatcagcag
     6121 caccacgttc tcctgctaca gtagagaaac gggcaaacat ttcggtttgc ttacctattt
     6181 cagagaatat tttagcattc gtatattttg ttatatcttt agttacagta aatgtcccaa
     6241 atgcaccaga acctttggca tgcattcgac gttctggtat tacttctcta tcaaattgag
     6301 acatttgctc taaaaagtaa atatcttgca ttaaaagagg tcccctaggc cctgctgtca
     6361 tactattttc tcggtctgat actggatgcc caaaaacacc agttaacttt ttgtcttgtt
     6421 gtgacatagt catccctcca caatttataa taattataat ttaatacata ttataattta
     6481 accccatcaa aattacaatt aaacatgctc aagatgatta aaaaatcaga aaattttaat
     6541 tttttcgctt tacagtacta aatagttatt gaatagtaca cttaaaaagc ttacaataat
     6601 gggtatattt aaatagatgg gagactaaat atggaagata ataaaatgaa ccgtagtctt
     6661 aactcaagac acatttccat gattgctata ggtggtgcaa ttgggactgg tctatttgta
     6721 gctactggta atatcatttc tcaagctggt cctggaggcg ctatactcgc ttatcttgtt
     6781 attggtgtca tgctatattt cttaatgtca tcaattggag agttggcaac attttatcca
     6841 gtatcaggtt cattcagctc ttattcaaca cgctttattg actcatctct tggctttacc
     6901 atgggatggt tgtattgggc attgtggtca ttagttacaa gtgttgatgt catagtagcg
     6961 tcaaatgtgc tttatttctg ggacacattt aaatttttcc accccattac ttggagctta
     7021 atctttatta caattttact attattaaac attttttctg taaaatcatt tggagaaact
     7081 gagttttggt tatcattgat taaagtgtta acaattatcg tattcgttat ttttggcttt
     7141 ttaatgattt tcggtatctt aggtggtcat acatatggat ttgaaaacta tacaaaaggc
     7201 caagcaccgt ttgttggtgg tatctctggt ttcttaggcg tattattagt cgccggattt
     7261 tcggttggtg gtacagaagt agtagcagta actgctggtg aatcagatga ccctaaaaag
     7321 tctatgccta aggcaattaa acaagtattt tggcgtattc ttttattcta tgtcttatca
     7381 attgcagtaa ttggtgcaat tattccgtac acagatccat cattattaag agcaagtagt
     7441 tcaataagtc aaagcccatt tacaattgta ttcgatagag taggcatagc ctttgcagca
     7501 tcagtaatca acgcggttat tttaacttca ttattatccg ctgcaaattc aggtgtttat
     7561 acaacaggca gaatgttgta ttccttaagt tcagacaaaa aagcacccca atttttaagt
     7621 aaattaaaca agacaactaa gttaccttta agagcattat taactactta tgcagtcgtt
     7681 gttattgtta ttatttatgc aaactttaat tcaaatgccg tttttaattt acttgaaatt
     7741 attggttcaa tgattatagt tgtttgggga tcaagcattt ggtcacaaat acgattgcga
     7801 caagctatta aaaaacaagg tcaagaccct aataaggtcc taccatataa agcacctttt
     7861 tatccattag gaccaatcat tgtcatcact acactattat tcttgctatt tggtggctca
     7921 gttgaatata ttttaaaaga tcaatggtta aatgctttta aaaacttttt acctttaatc
     7981 attctagcgt tgatttactt tattcataaa atcattcaca aaacaaaatt tgtaaagcta
     8041 gaaacaatta atttaaaacc acacgattat gacaatcaaa aataaataaa ttcttacacg
     8101 gcagatgctt tagattatct atagcatttg ccgcttttta atggtgtaaa gcagatatac
     8161 tttaatatct ttttct
//
LOCUS       DANBWH010000121         8154 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.124, whole genome shotgun sequence.
ACCESSION   DANBWH010000121 DANBWH010000000
VERSION     DANBWH010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8154
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.124"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            17..916
                     /locus_tag="PPY92_001837"
     CDS             17..916
                     /locus_tag="PPY92_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDJ2625826.1"
                     /translation="MGLVIEHVTKRFGKMTAVNDISLELESGKMLGFLGRNGAGKTTT
                     FRMILGLSEPTEGHITYNGKKLDKTMYNRIGYLPEERGLHGKLTVEEELKYLATLKGM
                     SKTEIQQQISYWLERFDITENRKKRIDSLSKGNQQKIQLLASMLHKPELLILDEPFSG
                     LDPVNVELLKEAVKDLNDWGSTIVYSSHRMEHVEELCDDVCILDKGQLVVSGDINHVR
                     ASNGNKKVVIESETTLPDLTNIRGIIHSENMKQGLQLTIENEDVAKDIYQVVAHQGYV
                     KRFQVVEPSLQDIFIEKVGGKDA"
     gene            909..2141
                     /locus_tag="PPY92_001838"
     CDS             909..2141
                     /locus_tag="PPY92_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDJ2625827.1"
                     /translation="MHKFWATFTLTYIKKIKAKSFVIFMIIIAALMIGLSNIDKIINM
                     FDDGPDKIGVAAPNEQIYKVFKQQANTFHSDAKFTKVSIEDAEKEVKKHKLDKAYIIK
                     VNQNRTLQGTIISEKRVSHEDSQKVQALLTAIQTNMVAGELNINKEDLQKLQAQSKVD
                     NKVISNDEVDKVSEGQKIFNYALAYGIIFLMFFIVLNYASQIAMEIASEKTSRVIEMI
                     ITSISPIQHIFAKILGVIAVAVTQIILIVLMAVICIFAFDLKDLLQGFNVEMNQLSWQ
                     IIIVGIISSIVGIMVYVLLAAILGSLTSRIEDLNQSLMPLTLLGMIAFYIAIFTINNP
                     DGQLALITSYIPFLAPFQLVVRAQTSGLQIYEVILSSLISIVMVAVLIWIAIKTYKDS
                     VLTFERGLFNSMKRVFKK"
     gene            2125..2310
                     /locus_tag="PPY92_001839"
     CDS             2125..2310
                     /locus_tag="PPY92_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625828.1"
                     /translation="MYLKNKIDESDTHLSYKMMMKACYSYFFIFSYRIMSKKVYNMNV
                     KTNFLKWECEVRRNVNK"
     gene            2359..2685
                     /locus_tag="PPY92_001840"
     CDS             2359..2685
                     /locus_tag="PPY92_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HDJ2625829.1"
                     /translation="MLYPIFIFILAGLCEIGGGYLIWLWLREGQSSLVGLIGGAILML
                     YGVIATFQSFPSFGRVYAAYGGVFIIMSLIFAMVVDKQMPDKYDVIGAIICIVGVLVM
                     LLPSRA"
     gene            2715..3734
                     /locus_tag="PPY92_001841"
     CDS             2715..3734
                     /locus_tag="PPY92_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDJ2625830.1"
                     /translation="MIVEVEHQRQGVDLIKIDNDETKIIFTNYGARVVSWKYHDNNIV
                     LGNVVEADEFYHSNPFKFGASIGRYSGRIDNAKFKLKGKEYQLEKNNGEHHLHGGCHG
                     LDNKLFDYEIRNEIAQIKVIFKTVLKSADDHFPGDIDVTITHIYDADHQWSIEYEAVA
                     SEDTLFSPMNHVYFNLNRDNNVVDNHRISSNQLDMYVLDERNIVTGDILDLHEVFEDN
                     KIKLSDIFTSQHAQLSQQMTRFGGLDHPFTVGEHKMYVENHEFMLEVDTDMPHVVFFT
                     FNQPDEWDSPFNIYKPHSGFTLETQFLPNDINLYGEKAPSILKAGEVFNSKTSFRIEE
                     KVEND"
     gene            complement(3970..4623)
                     /locus_tag="PPY92_001842"
     CDS             complement(3970..4623)
                     /locus_tag="PPY92_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HDJ2625831.1"
                     /translation="MISIHAISTGKIQDLPYSSKRPIRSALDKTKISQTTWLSSTGFT
                     GDEQAYKDHGGPHKAVCGFSKHNYALYQDDLPTLPTHAMFGENLTFDYLDESDVYFGN
                     QYRLSEALIEVSEIREPCWKIQAKYNIPDLVKRMSTSGKTGFYFRVLKQGYVSPNDQL
                     YLIQEAPIEHRLSVQQLNDLYYNDRQNQDMLRYALNNPFLSPTRRDKLQKMYNRTLK"
     gene            4797..5483
                     /locus_tag="PPY92_001843"
     CDS             4797..5483
                     /locus_tag="PPY92_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HDJ2625832.1"
                     /translation="MKDVKALKLMTLNDVLSQINGDMTLGIGTGSTMELLLPQMAQLI
                     KERGYNITGVCTSNKIAFLAKELGIKICEINDVDHIDLAIDGADEVDPSLNIIKGGGG
                     ALFREKVIDEMASRFVVVVDETKIVQYLGETFKLPVEVDKFNWYHILRKIESYADIKV
                     ERRVNEDVAFITDNGNYILDCKLPKGIDPYKFHEYLIHLTGVFETGYFLDMADQVIVG
                     TQEGVKILEK"
     gene            complement(5549..6808)
                     /gene="sdpC"
                     /locus_tag="PPY92_001844"
     CDS             complement(5549..6808)
                     /gene="sdpC"
                     /locus_tag="PPY92_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpC"
                     /protein_id="HDJ2625833.1"
                     /translation="MKNNKISGFQWAMTIFVFFVITMALSIMLRDFQSIIGVKHFIFE
                     VTDLAPLIAAIICILVFKYKKVQLAGLKFSISLKVIERLLLALILPLIILIIGMYSFN
                     TFADNFILLQSTGLSVPITHILIGHILMAFVVEFGFRSYLQNIVETKMNTFFASIVVG
                     LMYSVFSANTTYGTEFAAYNFLYTFSFSMILGELIRATKGRTIYIATTFHASMTFGLI
                     FLFSEEIGDLFSIKVIAISTAIVAVGYIGLSLIIRGIAYLTTRRNLEELEPNNYLDHV
                     NDDEETNHTEAEKSSSNIKDAEKTGVATASTVGVAKNDTENTVADEPSIHEGTEKTEP
                     QHHIGNQTESNHDEDHDITSESVESAESVKQAPQSDDLTNDSNEDEIEQSLKEPATYK
                     EDRRSSVVIDAEKHIEKTEEQSSDKNK"
     gene            7098..8033
                     /locus_tag="PPY92_001845"
     CDS             7098..8033
                     /locus_tag="PPY92_001845"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HDJ2625834.1"
                     /translation="MYKQGEPNLWTGRLDSETDPKKFRHFQTVTFEDLSKLEKSSMPS
                     GVGILGYAVDKGVALNKGRIGAKEGPDAIKQAFAGLPDLNQCETLVDYGNVYHDHEEL
                     IDTQKEFAMLAAKSIANHRQTFLLGGGHDIAYAQYLATRKVYPTQSIGVINIDAHFDT
                     RAEQQSTSGTSFRQILEEDENTDYLVLGIAQGGNTQSLFDYAKEKKIDYVFADELLSH
                     VSPTIKDMIERFVHEHDVIMFTICMDVIDSAFAPGVSAPAVLGLYPHTVLELAKRIIP
                     SDKVSSVSIAEMNPTYDADNRTAKLVANLVHHFLK"
BASE COUNT         2803 a         1098 c         1555 g         2698 t
ORIGIN      
        1 ttttaggagg caaagtatgg gacttgtaat tgaacatgtt acgaaacgtt ttggcaagat
       61 gacggcagta aatgatattt cattagagtt agaatcaggc aaaatgttag gttttctagg
      121 aagaaatggt gcaggtaaaa caacgacatt tcgtatgatt ttaggcttaa gtgaaccaac
      181 tgaaggacac attacgtata acggtaaaaa actagataaa acaatgtaca atcgtattgg
      241 ttatttaccg gaagaacgcg gtttacatgg gaaattgaca gttgaagaag aattgaaata
      301 tttggcaact ttaaagggaa tgtcaaaaac agaaatacaa cagcaaatat cgtattggct
      361 tgagcgtttt gatattactg aaaaccgcaa aaaacgaatt gatagtctat caaaagggaa
      421 tcaacaaaaa attcagctgt tagcaagtat gttacataaa ccagaattac taatattaga
      481 tgagcctttc agtggtctag acccagttaa tgttgagtta ttaaaagaag cagttaaaga
      541 cttaaatgat tggggcagta caatcgttta tagttctcac cgtatggagc atgttgaaga
      601 attatgtgat gatgtttgta ttttagataa aggtcaactt gttgtttctg gtgatatcaa
      661 tcatgtacgt gcatctaatg gtaacaagaa agttgtcatt gaatcagaaa caacactacc
      721 agatttaaca aacattagag gcatcattca tagtgaaaac atgaagcaag gtcttcagtt
      781 gacgattgag aatgaagacg tagccaaaga tatttatcaa gttgtggcac atcaaggtta
      841 tgttaaacga ttccaagttg tagaaccatc attacaagat atattcattg agaaggttgg
      901 tggcaaggat gcataagttt tgggcgacat ttactttgac atatatcaaa aaaattaaag
      961 ctaaatcatt tgtaattttt atgattatta ttgctgcctt aatgattggc ctatctaaca
     1021 ttgataagat tattaacatg tttgatgatg gtccagataa aattggtgta gcagcaccga
     1081 atgaacaaat ctataaggtt ttcaagcaac aggcgaatac attccatagt gatgctaagt
     1141 ttactaaagt ttctatagaa gatgctgaaa aagaagtaaa aaaacataaa cttgataaag
     1201 cttatattat caaggtgaat caaaatcgca cgttacaagg gacaatcatt agtgagaagc
     1261 gagtaagtca tgaagattca caaaaagtac aagcactgct tacagctatt caaacaaata
     1321 tggtagccgg ggaattaaat atcaacaaag aagacttaca aaaattacaa gcacaaagta
     1381 aagttgataa taaggtgatt tctaatgatg aagtcgataa agtatcagaa ggacaaaaga
     1441 ttttcaacta tgctttagct tatggcatta tctttctaat gttcttcatt gttttaaatt
     1501 atgcatctca aatagctatg gaaattgcta gtgagaaaac atcgcgtgtc attgaaatga
     1561 ttattacaag tatttcacca atccaacata tttttgctaa aattttaggc gttatagcag
     1621 ttgccgtgac tcagattatt ttaattgtat tgatggcagt tatatgcatt tttgcctttg
     1681 acttaaaaga cttgctacaa ggttttaacg tggaaatgaa tcaattgtct tggcaaatta
     1741 ttattgtcgg aattataagt agtattgttg gaattatggt gtatgtatta cttgcagcca
     1801 tcctcggatc attaacatct agaatagaag atttaaatca atctttgatg ccactgacat
     1861 tacttggtat gattgcattt tatatcgcga tattcacgat aaataatcct gatggacaac
     1921 ttgctttaat cacaagttat attccgtttt tagcgccatt tcaattagtt gtacgcgctc
     1981 aaacttcagg gcttcaaata tatgaggtca tcttaagttc tttaatctca attgtaatgg
     2041 tagctgtctt gatatggatt gcaattaaaa catataaaga ttcagtactt acatttgaac
     2101 gtggcttatt taattcaatg aaacgtgtat ttaaaaaata aaattgatga gagtgacaca
     2161 catttatcat ataaaatgat gatgaaagcg tgttactctt atttttttat atttagctat
     2221 agaataatgt ctaaaaaagt gtacaatatg aatgtgaaaa cgaacttttt aaaatgggaa
     2281 tgtgaagtaa gaagaaatgt aaacaaatga tatgtagccg atagattatt gatagcaaat
     2341 gtgtgatgga gtgatttcat gttatatccg atttttatat ttatattagc tggcttatgt
     2401 gaaataggtg ggggatacct gatttggctg tggcttaggg aaggacagtc ttcacttgtt
     2461 gggctaatag gcggtgcgat actcatgtta tatggtgtca ttgcgacatt tcaatcattt
     2521 ccatcattcg gaagagtata tgcagcatat ggtggcgtat ttatcatcat gagtttgatt
     2581 tttgcgatgg tcgtagataa acaaatgcca gataaatatg atgttatcgg tgcaatcatt
     2641 tgtattgtag gtgttttagt aatgttgcta cccagcagag cttgaacact tgtggttaga
     2701 agggagaagt aaaaatgata gttgaagttg agcaccaaag acaaggtgta gatttaatca
     2761 aaattgataa tgatgaaact aaaattattt ttacgaatta tggagcaaga gtagtgtctt
     2821 ggaaatatca tgataataat attgttcttg gaaatgttgt tgaagcggat gagttttatc
     2881 atagtaatcc ttttaaattt ggtgcttcta taggaagata tagcggtaga attgataatg
     2941 ctaaatttaa gttgaaaggt aaagaatatc agttagaaaa aaacaacggt gaacatcatt
     3001 tacatggtgg atgtcatggt ttggacaata agttatttga ttatgaaatt agaaatgaaa
     3061 ttgctcaaat taaagttata tttaagacgg tgttgaaatc agctgatgat catttcccgg
     3121 gtgatatcga tgtgacgatt acacatattt atgatgcgga tcaccaatgg tcgattgaat
     3181 acgaagcagt tgcatcagaa gacacattgt ttagtcctat gaatcatgtg tacttcaatt
     3241 tgaatcgaga taataatgtc gttgataatc atcgcatttc aagtaatcaa ttagatatgt
     3301 atgtattaga tgagcgaaat attgttacag gtgatattct agatttacat gaagtgtttg
     3361 aagataacaa gataaaattg agtgatattt ttacatcaca acatgcgcaa ttaagccaac
     3421 aaatgacacg ttttggtggt ctcgatcacc catttacagt tggtgaacat aaaatgtatg
     3481 ttgaaaatca tgaatttatg ttagaggtcg atacagatat gcctcatgtt gtgttcttta
     3541 cttttaacca gcctgatgag tgggatagtc catttaatat ttataaacca cattctggct
     3601 ttacattaga aacacaattt ttaccaaatg atattaattt atatggtgaa aaagcaccgt
     3661 caattttaaa agctggtgaa gtgttcaatt cgaaaacaag tttccgtatc gaagaaaaag
     3721 tagaaaatga ttaaaagtat cggcatttat ataatattta tgttatttgg cggaattttg
     3781 aataggttat caagaatcgt atcaaatgaa gtgacatcat taatatacag tttgaaataa
     3841 agaaaatata taacataaaa aaggaatcaa tagcatcagt ggttatagat aagactatta
     3901 tacaaacgat gtgtaagttg attccttttt gttttgaaaa gttcatttaa cacttataat
     3961 gaaaggtaat tatttcaatg ttctgttata cattttttga agtttatcgc gtcttgttgg
     4021 tgacagaaat ggattgttta gtgcatatcg taacatatct tgattttgtc tatcattata
     4081 ataaaggtca ttaagctgtt gtacagataa acgatgttcg attggtgctt cttgtattaa
     4141 gtaaagctga tcatttggag atacatagcc ttgttttaat acccggaaat agaaacctgt
     4201 tttaccagat gtagacatgc gcttcactaa atcaggaata ttatattttg cttgaatttt
     4261 ccagcatggt tctctaattt cagaaacctc aattaacgct tcacttaaac gatattgatt
     4321 accaaagtaa acatcagatt cgtctaaata atcaaatgtt aaattctctc caaacatcgc
     4381 atgagtaggt agtgtaggta aatcatcttg atacagtgca taattatgct tactaaaccc
     4441 acaaactgct ttatgtggtc caccatgatc tttataagcc tgttcatcac cagtgaaacc
     4501 agttgaagat aaccatgttg tttgtgaaat cttagtttta tctaaagcag agcgtatcgg
     4561 tcttttcgag ctataaggca aatcttggat ttttcctgtc gaaattgcgt gaattgatat
     4621 catatcattc tccttaacga gtattattta aaatatattg tactataaaa catttttaga
     4681 attgtaaatt ctgattgata tgtttaaaaa aatgttagaa ataagggtat gttaatttat
     4741 acgagtcatg taaaatatga attgaataga ttacataaat atgggaggct accttcatga
     4801 aagatgtcaa agcactcaag ttaatgacat taaatgatgt tttaagtcaa atcaatggtg
     4861 acatgacgct gggaattggt acaggaagta caatggaatt actcttacct caaatggcgc
     4921 aactaattaa agaacgtggt tacaatatta caggtgtatg cacttctaat aaaattgcat
     4981 ttttagcaaa agaattaggt attaaaatat gtgaaatcaa tgatgttgat catatcgact
     5041 tagcaattga tggtgctgat gaagtagatc catctttaaa tataattaaa ggtggcggtg
     5101 gtgcgctgtt cagagagaaa gttatagatg aaatggcatc gcgttttgtt gtggttgtcg
     5161 atgaaacgaa aatagttcaa tacttaggtg agacgtttaa gttaccagta gaggtggata
     5221 aatttaactg gtatcacata ttacgtaaaa ttgaatcata cgctgatata aaagtagaac
     5281 gtcgtgtaaa cgaagatgtc gcatttataa ctgataatgg caattatatt ttagattgta
     5341 agttaccaaa aggaattgac ccatataagt tccatgagta tttaattcat ctgacaggtg
     5401 tgtttgagac aggatacttc ttagatatgg cagatcaagt tattgtaggc actcaagagg
     5461 gtgttaaaat tttagaaaaa taatatatag tatagaaaag cgcttgctag catcatcttt
     5521 ttaagaggta ctagcaagcg ctttgttatt atttgttttt atctgaagat tgttcttcag
     5581 ttttttcgat atgtttttct gcatcaatta caactgatga acgtctgtct tctttataag
     5641 tcgcaggttc ttttaatgat tgctctattt catcttcatt tgaatcgttt gttaaatcgt
     5701 cactttgtgg tgcttgttta actgattctg ctgattctac tgactccgaa gtgatgtcat
     5761 gatcttcatc atgattagat tcagtttgat tacctatgtg atgttgaggt tctgtttttt
     5821 cagtaccttc atgaatgctt ggttcgtcag ccactgtatt ttcagtatca tttttagcaa
     5881 caccaaccgt tgatgcagta gctacacctg ttttttcagc atctttaata tttgaagaag
     5941 atttttcagc ctcagtatga ttagtttctt catcgtcatt gacatggtct aaataattat
     6001 taggctcaag ttcttcaagg tttcgtcttg ttgttaaata tgcaatacct cggataatta
     6061 agcttaaacc aatgtatcct actgcaacga ttgctgttga aatggcgatg actttgattg
     6121 aaaatagatc gccgatttct tcgctaaaca agaaaataag tccgaatgtc attgaagcat
     6181 ggaatgtcgt tgcaatataa attgtacgtc ctttagtcgc tctaattaat tcaccaagaa
     6241 tcatagagaa tgagaatgta taaaggaagt tataagcagc aaattctgta ccataagttg
     6301 tatttgctga gaaaactgaa tacatcaaac caacaacaat acttgcaaag aacgtgttca
     6361 ttttcgtttc aacaatattt tgtaagtatg aacggaatcc gaattctact acgaacgcca
     6421 tcagaatatg tccaatcaga atgtgtgtaa taggtactga taagcctgtt gattgtaata
     6481 aaataaagtt atctgcaaat gtattaaagc tgtacatacc aataattaga ataattaaag
     6541 gtaaaattaa agctagcaat agacgttcaa ttactttcag gctgattgag aattttaaac
     6601 ctgcaagttg gaccttttta tatttgaaaa cgagtataca aatgattgca gcaattaatg
     6661 gtgctagatc tgtaacttca aatataaagt gtttgacacc aattatagac tggaaatctc
     6721 tgagcataat ggataacgcc attgtaatga caaagaagac gaaaatcgtc attgcccatt
     6781 gaaaaccaga aattttattg ttcttcatta tatgtaacct ccattaggta acaagcaaaa
     6841 tatcttttag taacaattat acatattaca agccgagaga taaattgtct atgtcaatat
     6901 taatgaaaat gtaatagtct tgacgtattt tattaacata aatcagctaa gtattcgtta
     6961 ttacatagaa aataggggta aaagtaatgt agataagtat ttttaagata cggatttaac
     7021 tatgaattga attcaaaatt tcctatacgt tatactttaa ttgttaataa gccacataaa
     7081 taagaggggg aaatgctgtg tacaagcaag gtgaaccaaa tttatggact ggaaggttag
     7141 atagtgaaac agacccgaaa aaatttagac attttcaaac agtaacattt gaagatttgt
     7201 ctaagctgga aaagagtagt atgccatcag gggtcggtat attaggctat gctgttgaca
     7261 aaggtgttgc tttaaacaag gggcgcattg gtgcaaaaga aggaccagat gcgattaaac
     7321 aagcatttgc aggtttgccg gatttgaatc aatgtgaaac tttagtcgat tacggaaatg
     7381 tttatcatga tcatgaggaa ttaattgata ctcaaaaaga atttgctatg cttgcagcga
     7441 agtcaattgc taatcataga caaacatttt tattaggtgg tggacatgat attgcgtatg
     7501 ctcaatattt agcaacacgt aaagtctatc caacacaatc tattggtgtg ataaatattg
     7561 atgcgcattt tgatacacgt gctgaacaac aatctacatc tggaacgagc tttagacaaa
     7621 ttttagaaga agatgaaaac acagattatt tagtgcttgg tattgctcaa ggtggtaata
     7681 cgcaaagttt atttgattat gctaaagaga aaaagattga ttatgtcttt gcagatgaat
     7741 tattgagtca cgtatcacca acaattaaag atatgattga acgttttgta catgaacatg
     7801 atgtcattat gtttacgatt tgtatggatg tcattgatag tgcgtttgcg cctggggtaa
     7861 gtgcgccggc agtgttaggt ttgtatccac atactgttct tgaattagca aaacgtatta
     7921 ttccaagtga taaggtgtct tcagttagta ttgctgagat gaatccaaca tatgacgctg
     7981 acaatagaac tgctaagctc gttgctaatt tagtgcatca ttttttaaaa taattaaaaa
     8041 tatttatatg tgacgagatt tgcattgcag ggcagaatgt gagtatttct taatagaagt
     8101 tatattaacg ttgatatatt tgaaaaataa ttgcagtaag aagactctta agtt
//
LOCUS       DANBWH010000122         8102 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.126, whole genome shotgun sequence.
ACCESSION   DANBWH010000122 DANBWH010000000
VERSION     DANBWH010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8102
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.126"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            31..822
                     /gene="pgeF"
                     /locus_tag="PPY92_001846"
     CDS             31..822
                     /gene="pgeF"
                     /locus_tag="PPY92_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HDJ2625835.1"
                     /translation="MNDNFKKQPHHLIYEELLQQGITLGITTRGDGLSDYPKNAFNMA
                     RYIDDRPYNITQHQLQLAEEIAFDRKNWVFPIQTHENKVACITKDDIGTNIDTLTDAL
                     HGIDAMYTYDSNVLLTMCYADCVPVYFYSTKHHFIALAHAGWRGTYTEIVKEVLKHVN
                     FDLKDLHVVIGPSTSSSYEINDDIKNKFETLPIDSANYIETRGRDRHGIDLKKANAEL
                     LNYYGVPKENIYTTAYATSEHLELFFSYRLEKGQTGRMLAFIGQQ"
     gene            846..1514
                     /locus_tag="PPY92_001847"
     CDS             846..1514
                     /locus_tag="PPY92_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDJ2625836.1"
                     /translation="MKDNLQQISTQINDKSEKNNFSTKPNVIAVTKYVTIERAKEAYE
                     AGIRHFGENRLEGFFQKKEALPSDAVIHFIGSLQSRKVKDVINDVDYFHALDRLSLAK
                     EINKRAEHKIKCFLQVNVSGEASKHGIALEDVDQFIDDLKKYDKIEIVGLMTMAPLTD
                     DEAYIRSLFKQLRLKKEEIQRLNLEYAPCDELSMGMSNDYLIAVEEGATFVRIGTKLV
                     GEEE"
     gene            1511..2074
                     /locus_tag="PPY92_001848"
     CDS             1511..2074
                     /locus_tag="PPY92_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDJ2625837.1"
                     /translation="MSHLALKDLFSGFFVIDDEEEVEVPDKQQQVNEAPAKEQSQQTT
                     KQNAIKSVPQKSASRYTTTSEERNNRMSNYSKNNSRNVVTMNNATPNNASQESSKMCL
                     FEPRVFSDTQDIADELKNRRATLVNLQRIDKVSAKRIIDFLSGTVYAIGGDIQRVGTD
                     IFLCTPDNVEVAGSITDHIENMEHSFD"
     gene            2086..2376
                     /locus_tag="PPY92_001849"
     CDS             2086..2376
                     /locus_tag="PPY92_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDJ2625838.1"
                     /translation="MDINVLATIFKFILFVVEIYYFGMIIYFFTSWVPSIRETKVGYF
                     LAKIYEPFLQPFRKVIPPIGIIDISSIAAIIVLVLFQKGLLQIFNWILIQLQ"
     gene            2486..3265
                     /locus_tag="PPY92_001850"
     CDS             2486..3265
                     /locus_tag="PPY92_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDJ2625839.1"
                     /translation="MINIDIYQHFRQEEYELIDQLTDKCDQAEQHYAPVLTHFLDPRG
                     QYILEVICGSYEDLNVSFYGGPNAERKRAIISPNYYEPKESDFELTLMEIEYPEKFVT
                     LKHQHILGTLMSLGIEREQVGDIIVNERIQFVLTSRLESFIMLELQRIKGASVKLYTI
                     PVTDMIQSNENWKNESATVSSLRLDVVIKEMIRKSRTIAKQLIEKKRVKVNHTIVDSA
                     DFQLQANDLISIQGFGRAHITDLGGKTKKDKTHITYRTLFK"
     gene            3289..3906
                     /locus_tag="PPY92_001851"
     CDS             3289..3906
                     /locus_tag="PPY92_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDJ2625840.1"
                     /translation="MPFTPNEIKNKEFSRVKNGLEPTEVANFLEQLSTEIERLKEDKK
                     QLEKVIEERDTNIKSYQDVHQSVSDALIQAQKAGEETKQAAEKQAEAIIAKAEAQANQ
                     MVGDAVEKARRLAFQTEDMKRQSKVFRSRFRMLVEAQLDLLKNEDWDYLLNYDLDAEQ
                     VTLENIHHLHENDLKPDEVAANAQNNASNTPDNNQQSNDSETTKK"
     gene            4127..6880
                     /gene="ileS"
                     /locus_tag="PPY92_001852"
     CDS             4127..6880
                     /gene="ileS"
                     /locus_tag="PPY92_001852"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDJ2625841.1"
                     /translation="MDYKETLLMPKTDFPMRGGLPNKEPQIQEKWDAEDQYHKALEKN
                     KGNETFILHDGPPYANGNLHMGHALNKILKDFIVRYKTMQGFYAPYVPGWDTHGLPIE
                     QALTKKGVDRKKMSTAEFREKCKEFALEQIELQKKDFRRLGVRGDFNDPYITLKPEYE
                     AAQIRIFGEMADKGLIYKGKKPVYWSPSSESSLAEAEIEYHDKRSASIYVAFDVKDDK
                     GVVDADAKFIIWTTTPWTIPSNVAITVHPELKYGQYNVNGEKYIIAEALSDAVAEALD
                     WDKASIKLEKEYTGKELEYVVAQHPFLDRESLVINGDHVTTDAGTGCVHTAPGHGEDD
                     YIVGQKYELPVISPIDDKGVFTEEGGQFEGMFYDKANKAVTDLLTEKGALLKLDFITH
                     SYPHDWRTKKPVIFRATPQWFASISKVRQDILDAIENTNFKVNWGKTRIYNMVRDRGE
                     WVISRQRVWGVPLPVFYAENGEIIMTKETVNHVADLFAEHGSNIWFEREAKDLLPEGF
                     THPGSPNGTFTKETDIMDVWFDSGSSHRGVLETRPELSFPADMYLEGSDQYRGWFNSS
                     ITTSVATRGVSPYKFLLSHGFVMDGEGKKMSKSLGNVIVPDQVVKQKGADIARLWVSS
                     TDYLADVRISDEILKQTSDVYRKIRNTLRFMLGNINDFNPDTDSIPESELLEVDRYLL
                     NRLREFTASTINNYENFDYLNIYQEVQNFINVELSNFYLDYGKDILYIEQRDSHIRRS
                     MQTVLYQILVDMTKLLAPILVHTAEEVWSHTPHVKEESVHLADMPKVVEVDQALLDKW
                     RTFMNLRDDVNRALETARNEKVIGKSLEAKVTFASNDKFNASEFLTSFDALHQLFIVS
                     QVKVVDKLDDQATAYEHGDIVIEHADGEKCERCWNYSEDLGAVDELTHLCPRCQQVVK
                     SLV"
     gene            7171..7968
                     /locus_tag="PPY92_001853"
     CDS             7171..7968
                     /locus_tag="PPY92_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDJ2625842.1"
                     /translation="MYHNSNANFVNGITLNVRDKNELKPFYEDVLGLNIINETLTSIQ
                     YEVGQNNHVITLVELQNGREPLMSEAGLFHIAIKLPQISDLANLLIHLSEYDIPVNGG
                     IQPASLSLFFEDPEGNGFKFYVDKDEAQWTRQNDLVKIDIRPLNVPRLVSHATKLLWL
                     GIPDDAIIGALHIKTIHLSEVKEYYLDYFGLEQSAYMDDYSIFLASNGYYQHLAMNDW
                     VSATKRVENFDTYGLAIVDFHYPETTHLNLQGPDGIYYRFNHIEVED"
BASE COUNT         2961 a         1131 c         1537 g         2473 t
ORIGIN      
        1 taatttaaat gaaatgatgg tgacgatcga gtgaatgata attttaaaaa gcaaccgcat
       61 catttaatat atgaagaatt attacaacaa ggtattactc taggtattac aactagagga
      121 gatggtttaa gcgactatcc taaaaatgct tttaatatgg cgagatatat tgatgatcgc
      181 ccatataata ttactcaaca tcaattgcaa ttagctgaag aaattgcgtt tgatagaaaa
      241 aattgggtgt ttcccatcca aacacatgaa aataaagtcg cttgtattac aaaggatgat
      301 ataggcacaa atatagacac tttaactgat gcgcttcatg gtattgatgc gatgtacaca
      361 tatgatagta atgtcttatt aacgatgtgt tatgcagact gtgtaccagt atatttttat
      421 agtacaaaac atcattttat tgcattggcg catgcaggtt ggcgtggtac ctatactgaa
      481 attgtaaaag aagtgctaaa acatgtgaac tttgatttga aagacttaca tgtcgttatt
      541 ggaccatcta catcatcaag ttatgaaatt aatgatgata ttaaaaataa atttgaaaca
      601 ttgccaattg atagtgccaa ctatattgaa actagaggac gagatcgtca tggtattgat
      661 ttgaaaaaag ccaatgctga attattaaat tattatggtg ttcctaaaga aaatatttat
      721 acgacagcgt atgctacatc tgaacattta gaattatttt tctcttatcg attagaaaaa
      781 ggtcaaacag gacgcatgtt agcattcatt ggtcaacagt aaacaaggag gagatatgtt
      841 tgcgtgtgaa agataattta caacaaatct caacacaaat taatgacaaa agtgaaaaaa
      901 ataatttttc aacaaaacca aacgtgattg cagttacaaa atatgttaca atagagcgag
      961 ctaaagaagc gtatgaggct ggaataagac attttggtga gaatagattg gaaggctttt
     1021 ttcaaaagaa agaagcatta ccatcagatg cggtgattca ttttatagga tcattacaat
     1081 ctcgaaaagt taaggacgtt ataaacgacg tagattattt ccatgcttta gatcgattga
     1141 gcttagccaa agaaattaac aaacgtgcag aacataaaat taaatgtttc ttgcaagtga
     1201 acgtttcggg agaagcttct aaacatggta ttgctttaga agatgttgat cagtttatag
     1261 atgatcttaa aaaatatgac aaaatcgaaa ttgtaggttt aatgacgatg gcaccattga
     1321 cagatgatga agcatatatt agatcgttat ttaaacagtt acgtttgaaa aaagaagaaa
     1381 tacaacgact caatttagaa tatgcgcctt gtgatgaatt atcaatggga atgagtaatg
     1441 actatcttat tgcagttgaa gaaggtgcga cgtttgttag aattgggact aaacttgtag
     1501 gagaagagga gtgagccact tggctttaaa agatttattt agtggatttt ttgtaataga
     1561 tgatgaagag gaagtagaag tacctgacaa acaacaacag gtcaatgaag cgccagcaaa
     1621 agagcagtca caacaaacaa caaaacaaaa cgcaatcaaa tcagtccctc aaaaatctgc
     1681 atcaagatat acaacaacgt cagaagaaag gaataaccgt atgtctaatt attcaaaaaa
     1741 taattcacgt aatgttgtaa ctatgaacaa tgctacacca aacaatgcat cacaagaaag
     1801 ttcaaaaatg tgtttattcg aaccacgtgt tttttcagat acacaagata ttgctgatga
     1861 gcttaaaaac cgccgtgcga cacttgtcaa tttacaacgt attgataaag tatcagcgaa
     1921 aagaattatt gattttttaa gcggtactgt ttatgcaatc ggtggagata tccaacgtgt
     1981 aggtactgat attttcttat gtacgcctga taatgtggaa gtagctggaa gcattacaga
     2041 ccatattgaa aatatggaac attcattcga ctaaggagtt aacatatgga tataaatgtg
     2101 ctagctacaa tatttaaatt tatccttttt gttgttgaaa tttattattt cggcatgatt
     2161 atatatttct ttacatcttg ggtaccaagt attagagaaa ctaaggtagg ttatttttta
     2221 gcgaaaatat atgaaccttt cttacaacca tttagaaaag taattccacc tattggaatt
     2281 atcgacatat catcaatcgc tgcaattatc gttttagtat tattccaaaa agggttactc
     2341 caaatcttta attggatttt aattcaatta caataaatta agcgacataa cgaggcaagg
     2401 gtacatgata ctttagcctc gtttttgata tgtatttttc tgaatataag ggcaatagat
     2461 ggtattttat aattttttta aggtagtgat taacatagat atttatcaac actttagaca
     2521 ggaagaatac gaattaattg atcagctaac ggataaatgt gatcaagcgg aacaacatta
     2581 tgcaccagta ttaacgcatt ttttagatcc aagagggcaa tatatattgg aagtgatttg
     2641 tggcagttat gaagatttaa acgtctcttt ttatggcggg cctaatgctg aaagaaaaag
     2701 agcaatcatt tcgccgaact attatgaacc taaagaaagt gactttgaat taactttaat
     2761 ggaaatagag tatcctgaaa aattcgtcac tttaaaacat caacatattt tagggacatt
     2821 aatgtcttta ggtatcgaac gcgaacaagt tggagatata attgtgaatg aacgaattca
     2881 atttgttttg acaagtagat tggaatcatt tattatgtta gaattacaac gtattaaagg
     2941 cgcatcagtt aaactttata ctattccagt aacagatatg atacaatcta atgagaattg
     3001 gaaaaatgaa agtgcaacag ttagttcttt aaggttagat gttgttatta aagaaatgat
     3061 acgtaaatca cgtacgattg cgaaacaact aattgaaaaa aaacgtgtta aagtgaatca
     3121 cactattgtt gattcagcag attttcaatt acaagcaaat gatttaatat ccatccaagg
     3181 ttttggtaga gcacacatta ctgacttagg tggtaaaact aaaaaagata aaacgcacat
     3241 tacctataga acattattca aatagtaatg atttaaggag gataacaaat gccttttaca
     3301 ccaaatgaaa ttaagaataa agagttttca cgtgtaaaga atggtttaga acctactgaa
     3361 gttgctaatt ttttggagca actaagcact gaaattgaac gtcttaaaga agataaaaaa
     3421 caacttgaaa aagtaatcga agagagagat actaatatta agtcttatca agacgtgcat
     3481 caatctgtaa gtgatgcttt gatacaagct caaaaagctg gtgaagaaac taagcaagct
     3541 gcagagaaac aagctgaagc gattatagct aaggcagaag cgcaagctaa tcaaatggtt
     3601 ggtgacgcgg tagaaaaagc acgccgttta gcattccaga ctgaagatat gaaacgtcaa
     3661 tcaaaagtat ttagatcgcg tttccgtatg ttagttgaag cgcaattaga cttattaaaa
     3721 aacgaagatt gggattactt gttgaattat gatttagacg ctgaacaagt gacgcttgaa
     3781 aatattcatc atttgcatga aaatgattta aagccagatg aagttgcagc aaatgcacaa
     3841 aataatgcat caaatacacc agacaataat caacaatcca atgattcaga aacaactaag
     3901 aagtaagaat taaataaaga cagacgcgta atatacattt aacttttcac agcgaattag
     3961 gtaatggtga gagcctagta aaagcatgta tgttatatca ctggcttttt aatatttaaa
     4021 taatgtaatg agagaactct aagttgagtt aataagggtg gtaccgcgag caatcgtccc
     4081 ttttaattta acttagagtt ttttaaattt ttaaggagtg aaaaaaatgg attacaaaga
     4141 aacgttatta atgcctaaaa cagatttccc aatgcgaggt ggtttaccaa acaaggaacc
     4201 gcaaattcaa gaaaaatggg atgcagaaga tcaataccat aaagcgttag aaaaaaataa
     4261 aggtaacgaa acattcattt tacatgatgg cccaccatac gcgaatggta acttacatat
     4321 gggacatgcc ttgaacaaaa ttttaaaaga ctttattgta cgttataaaa ctatgcaagg
     4381 gttctatgca ccatacgtac caggttggga tacacatggt ttaccaattg aacaagcatt
     4441 aacgaaaaaa ggtgttgacc gaaagaaaat gtcaacagct gaattccgtg agaaatgtaa
     4501 agaatttgct ttagaacaaa ttgaattaca gaaaaaagat tttagacgtt taggtgttcg
     4561 tggtgacttt aatgatccat atattacatt aaaacctgaa tacgaagctg cacaaattcg
     4621 tatttttgga gaaatggcag ataaaggttt aatttataaa ggtaaaaagc cagtttattg
     4681 gtctccttca agtgagtctt cattagcaga agcagaaatt gaatatcacg ataaacgttc
     4741 agcatcaatt tacgttgcat ttgacgttaa agatgacaaa ggtgtcgttg atgcagatgc
     4801 taaatttatt atctggacaa caacgccatg gacaattcca tcaaatgttg cgattaccgt
     4861 tcatcctgaa ttaaaatatg gtcaatacaa tgtaaatggc gaaaaatata ttattgcaga
     4921 agccttgtct gacgctgtag cagaagcact ggattgggat aaagcatcaa tcaaattaga
     4981 aaaagaatac acaggtaaag aattagagta tgttgtagca caacatccat tcttagacag
     5041 agaatcgtta gtgattaatg gtgatcatgt tactacagat gctggtacag gttgtgtaca
     5101 tacagcacca ggtcacgggg aagatgacta tattgttggt caaaaatatg aattgccagt
     5161 aattagtcca atcgatgata aaggtgtatt tactgaagaa ggcggccaat ttgaagggat
     5221 gttctatgat aaagctaata aagccgttac tgatttatta acagaaaaag gtgcactatt
     5281 aaaattagac tttattacac atagctatcc acacgactgg agaacaaaaa aacctgtaat
     5341 cttccgtgct acaccacaat ggtttgcctc aatcagtaaa gtaagacaag atattttaga
     5401 tgcaatcgaa aatacaaact tcaaagtaaa ttggggtaaa acacgtattt acaatatggt
     5461 tcgtgaccgt ggcgaatggg ttatttctcg tcaacgtgtg tggggtgtac cgttaccagt
     5521 attttatgct gaaaatggcg aaattatcat gacgaaagaa acagtgaatc atgttgctga
     5581 tttatttgca gaacacggtt caaatatttg gtttgaaaga gaagcgaaag acttactacc
     5641 agaaggattt acacatccag gcagccctaa cggtacattt actaaagaaa cagacattat
     5701 ggacgtttgg tttgattctg gttcatcaca ccgtggcgtg ttggaaacaa gaccggaatt
     5761 aagtttccca gcggatatgt atttagaagg tagtgaccaa tatcgtggtt ggttcaactc
     5821 ttctatcaca acttcagttg ctacaagagg agtatcacct tataaattct tactttctca
     5881 tggttttgtt atggacggtg aaggtaagaa aatgagtaaa tctttaggta atgtgattgt
     5941 acctgaccaa gtggttaaac aaaaaggtgc tgatattgcg agactttggg taagtagtac
     6001 ggactattta gctgatgtta gaatttctga tgaaatttta aaacaaacat ctgatgttta
     6061 tcgtaaaatc agaaatacat taagatttat gttaggtaac attaacgatt tcaatcctga
     6121 cacagatagc attcctgaat cagagttatt agaagtggat cgttacttgc taaatcgttt
     6181 acgtgaattt actgcaagta cgattaacaa ctatgaaaac tttgactact taaatattta
     6241 tcaagaagtt caaaacttta tcaatgttga gttaagtaat ttctatttgg attacggtaa
     6301 agatatttta tatattgaac aacgtgattc tcatatccgt cgtagtatgc aaacagtgtt
     6361 atatcaaatt ttagttgata tgacgaagtt gttagcacca atcttagtgc atacagctga
     6421 agaagtttgg tctcatacac cacatgttaa agaagaaagt gttcacttag cagacatgcc
     6481 taaagttgta gaagtagatc aagctttatt ggataaatgg cgtacattta tgaatttacg
     6541 tgatgatgtg aaccgtgcat tagaaactgc tcgtaatgaa aaagttattg gtaaatcatt
     6601 agaagctaaa gttacgtttg ctagtaacga taaatttaat gcatctgaat tcttaacttc
     6661 atttgatgca ttacatcaat tatttatcgt gtcacaagtt aaagttgtag ataagttaga
     6721 tgatcaggca acagcttatg aacatggtga tattgtcatc gaacatgcag atggtgaaaa
     6781 atgtgaaaga tgttggaact attcagagga tcttggtgct gttgatgaat tgacgcatct
     6841 atgtccacga tgccaacaag ttgtaaaatc actggtataa ttgaaattgt ataaagtact
     6901 tatacagatg atataaatta aagctctctt cataatcatg ttgtagtttt tgttgacatg
     6961 atgaagagag tttttttgtg aataaaaaaa tgaccaagtt actggttata tgtgttaaaa
     7021 aatgtgtaat ttactaaaat aaaaattatt caggaatggt acaaattctc tgaagcttat
     7081 aaatgcgtta tagttgctat tctcaattat gttcgcgata attttaagta aaagtaagca
     7141 cagatattga atttgatagg agttaattga atgtatcata acagtaacgc aaactttgta
     7201 aatggtatca ctttaaatgt gagagataag aatgaattaa agccatttta tgaggacgta
     7261 ttaggattaa atattataaa tgagacatta acatcgatac aatatgaagt aggtcaaaat
     7321 aatcatgtca ttacacttgt tgaattacaa aatggacgtg aacctttaat gtccgaagcg
     7381 ggactgtttc atatcgcaat taaactacct caaattagtg atttagctaa tttactaatt
     7441 catttaagcg aatatgatat tccagttaac ggaggtatac agcctgcttc gttatcatta
     7501 ttttttgaag acccagaagg aaacggtttt aaattttatg ttgataaaga cgaagcgcaa
     7561 tggacgaggc aaaatgattt agtaaaaatt gatattagac cattaaatgt accgagatta
     7621 gtgagtcatg caacaaaatt gttatggtta ggtattccag atgacgctat tataggtgca
     7681 ttgcatatta agacaattca tttatcagag gtaaaagagt actacctcga ttattttgga
     7741 ttagagcaat cggcatatat ggatgattat tcaatatttt tagcatcgaa tggctattat
     7801 caacatttgg ccatgaatga ttgggtatca gcaacgaaac gtgtagaaaa ttttgatacg
     7861 tatggattag caattgttga ctttcattat cctgaaacaa cacatttaaa tttacaaggt
     7921 ccggatggta tctattatcg ctttaatcat atcgaagttg aagattagta tatactttga
     7981 atggacgaac catataatga atcgttttta atgatctttt tatacaagtt atgaaggagg
     8041 ctgggacatt aagttcttag gcaatgtaaa aagctgattt ctattaatta tttgatagaa
     8101 at
//
LOCUS       DANBWH010000123         8044 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.127, whole genome shotgun sequence.
ACCESSION   DANBWH010000123 DANBWH010000000
VERSION     DANBWH010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8044
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.127"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(165..1706)
                     /gene="guaA"
                     /locus_tag="PPY92_001854"
     CDS             complement(165..1706)
                     /gene="guaA"
                     /locus_tag="PPY92_001854"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDJ2625843.1"
                     /translation="MEMAKEQELILVLDFGSQYNQLITRRIREMGVYSELHDHEISIE
                     EIKKMNPKGIILSGGPNSVYEEGSFTIDPEIYNLGIPVLGICYGMQLTTKLLGGKVER
                     ANEREYGKAIINAKSDELFAGLPAEQTVWMSHSDKVIEIPEGFEVIADSPSTDYAAIE
                     DKKRRIYGVQFHPEVRHTEYGNDLLNNFVRRVCDCKGQWTMENFIEIEIEKIRQRVGD
                     RRVLCAMSGGVDSSVVAVLLHKAIGDQLTCIFVDHGLLRKGEGDMVMEQFGEGFNMNI
                     IRVNAKDRFMNKLKGVSDPEQKRKIIGNEFVYVFDDEASKLKGVDFLAQGTLYTDVIE
                     SGTKTAQTIKSHHNVGGLPEDMEFELIEPINTLFKDEVRKLGIELGIPEHLVWRQPFP
                     GPGLGIRVLGEITEDKLEIVRESDAILRQVIREEGLEREIWQYFTVLPNIQSVGVMGD
                     YRTYDHTVGIRAVTSIDGMTSDFARIDWEVLQKISSRIVNEVDHVNRVVYDITSKPPS
                     TIEWE"
     gene            complement(1731..3197)
                     /gene="guaB"
                     /locus_tag="PPY92_001855"
     CDS             complement(1731..3197)
                     /gene="guaB"
                     /locus_tag="PPY92_001855"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDJ2625844.1"
                     /translation="MWESKFAKESLTFDDVLLIPAQSDILPKDVDLSVQLSDKVKLNI
                     PVISAGMDTVTESKMAIAMARQGGLGVIHKNMGVEEQADEVQKVKRSENGVISNPFFL
                     TPEESVYEAEALMGKYRISGVPIVDNKEDRNLVGILTNRDLRFIEDFSIKIVDVMTQE
                     NLITAPVNTTLEEAEKILQKHKIEKLPLVKDGRLEGLITIKDIEKVIEFPNAAKDEHG
                     RLLVAAAIGISKDTDIRAQKLVEAGVDVLVIDTAHGHSKGVIDQVKHIKKTYPEITLV
                     AGNVATAEATKDLFEAGADIVKVGIGPGSICTTRVVAGVGVPQITAIYDCATEARKHG
                     KAIIADGGIKFSGDIIKALAAGGHAVMLGSLLAGTEESPGATEIFQGRQYKVYRGMGS
                     LGAMEKGSNDRYFQEDKAPKKFVPEGIEGRTAYKGALQDTIYQLMGGVRAGMGYTGSH
                     DLRELREEAQFTRMGPAGLAESHPHNIQITKESPNYSF"
     gene            complement(3235..4503)
                     /locus_tag="PPY92_001856"
     CDS             complement(3235..4503)
                     /locus_tag="PPY92_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDJ2625845.1"
                     /translation="MKNLILSVQHLLAMYAGAILVPIIVGTSLKFTPEQIAYLVTVDI
                     FMCGVATFLQANKVTGTGLPIVLGCTFTAVAPMILIGQTKGIDVLYGSLFLSGILVII
                     IAPFFSHLVKFFPPVVTGSVVTIIGINLMPVAMNYLAGGQGAKDYGDVKNILLGLMTL
                     IIILVLQRFTTGFIKSIAILIGLVLGTIGAGLLGMVDINQVNHAGWLGIPVPFRFSGF
                     SFDVTSTLVFFILAIVSLIESTGVYHALSEITGKKLERKDFRKGYTAEGLAIVLGSIF
                     NSFPYTAYSQNVGLVSLSGAKKNNVIYGMVVLLLICGCIPKLGALANIIPLPVLGGAM
                     IAMFGMVMAYGVSILGHIDFKNQNNLLIIAVSVGLGTGISAVPQAFKGLGEQFAWLTQ
                     NGIVLGAISAIILNFFFNGIKYKQTEENVK"
     gene            complement(4503..5081)
                     /gene="xpt"
                     /locus_tag="PPY92_001857"
     CDS             complement(4503..5081)
                     /gene="xpt"
                     /locus_tag="PPY92_001857"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDJ2625846.1"
                     /translation="MELLGQKVKEDGVVIDEKILKVDGFLNHQIDAKLMNEVGRTFYE
                     QFKDKGITKILTIEASGIAPAIMAALHFDVPCLFAKKAKPSTLTDGYYETSIHSFTKN
                     KTSTVIVSKEFLSEEDTVLIIDDFLANGDASLGLYDIAQQANAKTAGIGIVVEKSFQN
                     GHQRLEEAGLTVSSLCKVASLEGNKVTLVGEE"
     regulatory      complement(5312..5414)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5594..6001
                     /locus_tag="PPY92_001858"
     CDS             5594..6001
                     /locus_tag="PPY92_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDJ2625847.1"
                     /translation="MADITVVNDTGELYNVINQKKSEGYLESELTIISKSKLHLNDLH
                     DSEISLISTSGTFSDRMTKLLTGEDGEHAVLSRYNLAPDELEKYKQLILDDKMLVVAV
                     RDKSSHKEVQEHNSAYEEIDITHFAEASKGPKA"
     gene            6144..6806
                     /locus_tag="PPY92_001859"
     CDS             6144..6806
                     /locus_tag="PPY92_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625848.1"
                     /translation="MEFKTIQSVKDPHYNEALKLYDTKLNLSLTEDKRIIKRSLENNK
                     TENDYVFIVGLENDVVVSLATAHYEATTNSAFLIYLFAKDLPNHDELMTQTLHQIEHE
                     LNLLSNLVHSRSINFLMMEVPKEPADLTQDNGLIIEHRRQFLYEHQFERQTEIDYIHP
                     TNDGHTKKVDLFLKKNIKLTKDIYGTSVKSNYILKYVFANGIAREIIYALLEEMDLRK
                     PF"
     gene            6924..7880
                     /locus_tag="PPY92_001860"
     CDS             6924..7880
                     /locus_tag="PPY92_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625849.1"
                     /translation="MLTKEFAQRVELSEKQVRKIVQHLEERGYQLSKTEYRGREATDF
                     KEEDIELFKDIADKVKQTNSYDLAFDELEKEKDFLQVIVKNDDKNLPTNQNVAQLVED
                     LRLEIQKMREERHLLGQMMNQVHQQQQELKELQNQLTSKIDSNSESLKAIQTSQEAIQ
                     EAQASQAKVLAESTNKVEKNAVTEDKADSKDSKVAGVNTSTDAKTDTKAENAGDGTAT
                     KVDKEDQISATEAIEKASVEQSKNENAAETSNKEATVDADAQHDAEQQVAEAHAEASK
                     QATSNDSLEAKAENDSTASQSEMSEPKPQEEKKGFFARLFNL"
BASE COUNT         2713 a         1563 c         1243 g         2525 t
ORIGIN      
        1 taaacaatat gaaattttat ataaaaataa acacggaaaa ttgccttatt gatgaattaa
       61 aacgcaaact tagaacacct tacccctttt ccgtccttat tgtaattttc caactactca
      121 aattaaaaac cacccgtgca aacaggtggt tttattatat ataattattc ccactcaatt
      181 gtgcttggtg gttttgatgt aatgtcatag actacgcgat tgacgtggtc tacttcgttt
      241 acgatacgac ttgaaatctt ttgtaagact tcccaatcga tgcgtgcgaa gtcacttgtc
      301 atgccgtcga tagatgttac tgcacggata cctactgtgt gatcatacgt acggtagtct
      361 cccataacac ctactgattg aatgtttggt aacactgtga agtattgcca aatttctctt
      421 tcaagacctt cttctctaat cacttggcgt aaaatcgcgt ctgattctct aacgatttct
      481 agtttatctt cagtaatttc tccaagtaca cgaataccaa gaccaggtcc tgggaatggt
      541 tgtctccaca ctaaatgttc tggaatacct aattcaatac ctaatttacg tacttcatct
      601 ttaaacaatg tattgattgg ttcgattaat tcgaattcca tgtcttctgg taatccacca
      661 acattgtggt gtgatttgat tgtttgtgct gtcttagtac ctgattcgat gacgtctgta
      721 tatagtgttc cttgcgcaag gaagtctaca cctttaagtt ttgatgcttc atcatcaaat
      781 acatatacaa attcattacc aatgatttta cgtttttgtt caggatctga aacacctttt
      841 aatttattca taaaacgatc tttcgcatta acacgaataa tattcatgtt gaacccttca
      901 ccgaattgtt ccataaccat gtcgccttca cctttacgaa gtaagccatg gtctacaaag
      961 atacatgtta gttgatcccc aattgcttta tgcaataata cagcaacaac agatgaatct
     1021 acgccaccac tcatcgcaca taatacacga cggtcgccta cgcgttgacg aatcttttca
     1081 atttcaattt cgataaagtt ttccatcgtc cattgtcctt tacaatcaca aacacgacgt
     1141 acgaagttgt ttaataaatc attaccatat tctgtatgac gtacttctgg atggaattgc
     1201 acaccataaa tgcgacgttt cttatcttca attgctgcat agtctgtgct tgggctatca
     1261 gcgataactt caaagccttc tggaatttca ataactttat cagaatgact catccaaaca
     1321 gtttgttctg ctggtaagcc agcgaataac tcatctgact tcgcattaat gattgcttta
     1381 ccgtattcac gttcattggc acgttcaact ttaccaccta ataatttagt agttaattgc
     1441 atgccgtaac aaataccaag tactggaatt cctaaattat atatttccgg atcaattgta
     1501 aatgaacctt cttcataaac tgaatttgga ccacctgata agatgatacc ttttggattc
     1561 attttcttaa tttcttcaat tgaaatttca tgatcgtgta attcactata aacgcccatt
     1621 tcacgaattc ggcgtgtaat taattggttg tattggctac caaagtctaa gacaaggatt
     1681 aactcttgtt cttttgccat ttccatattt gtcgttctcc tttatcttaa ttagaatgag
     1741 tagttcggtg attctttcgt aatttgaata ttatgtggat ggctttctgc taaaccagca
     1801 ggacccatac gtgtaaattg tgcttcttcg cgtaattctc ttaaatcgtg tgaaccagta
     1861 taacccatac cagcacgcac accgcccatt aattggtaaa ttgtatcttg taacgcacct
     1921 ttataagccg tacgtccttc gataccttca ggaacaaatt tcttaggcgc tttgtcctct
     1981 tggaagtaac ggtcgtttga acctttttcc atcgcaccta aagagcccat accacggtat
     2041 actttatatt gtctaccttg gaaaatttct gtagcacctg gactttcttc agtacctgct
     2101 aataagctac ctaacataac cgcatgtcca ccagcagcta atgctttaat gatatctcct
     2161 gagaatttaa taccaccatc agcaatgata gctttaccat gtttgcgtgc ttcagttgca
     2221 caatcataaa ttgctgtaat ttgtggtaca ccaacacctg ctacaacacg cgtcgtacaa
     2281 attgaacctg ggccaatacc aactttaaca atatctgcac ccgcttcaaa taaatctttt
     2341 gttgcttctg cagttgctac gttaccagct actaatgtga tttctgggta agtcttctta
     2401 atatgtttca cttgatcgat aacgccttta gagtgaccat gtgctgtatc gataactaag
     2461 acatccacac ctgcttcgac taatttttga gcacgaatat cagtatcttt tgaaatacca
     2521 attgctgcgg ctacaagtag acgaccatgt tcatcttttg ctgcattagg gaattcgata
     2581 actttttcaa tatctttaat agtaataaga ccttctagac gtccgtcttt aactaatggt
     2641 aacttttcaa tcttatgttt ttggagaatt ttttctgctt cttcaagtgt tgtattcact
     2701 ggagctgtaa ttaaattttc ttgcgtcatt acatctacaa ttttaattga gaagtcttca
     2761 ataaaacgta agtcgcggtt tgttaaaata cctactaagt tgcgatcttc tttattatca
     2821 acaattggta cacctgaaat acggtattta cccattaatg cttccgcttc ataaacactt
     2881 tcttctggtg ttaagaaaaa tgggtttgaa atgacaccat tttctgagcg ttttactttt
     2941 tgaacttcgt ccgcttgttc ttcaacgccc atatttttat gaataacacc taaaccacct
     3001 tgacgagcca tagcaatcgc cattttagat tcagttactg tatccatacc agcagaaata
     3061 actggaatat ttaatttaac tttgtctgat aattgtacgc ttaaatcaac gtctttcggt
     3121 aaaatatcag attgtgctgg aattaataac acatcatcaa acgttaatga ttcttttgca
     3181 aatttacttt cccacattaa aaacagcctc catttttcaa attaattagt tatattattt
     3241 cacattttct tccgtttgtt tatactttat tccattaaaa aagaaattaa gaataattgc
     3301 agagattgcg cctaaaacaa ttccgttttg agtcaaccat gcaaattgtt cacctaaacc
     3361 tttaaatgct tgtggtacag cgcttatacc agtacctaat cctactgata cagcaataat
     3421 taataaattg ttttgatttt taaaatcgat atgtcctaat atactaacac catatgccat
     3481 taccatgcca aacatagcta tcatcgcacc acctaacaca ggtagcggta tgatatttgc
     3541 taatgcacca agcttaggta tacaaccaca tataagtaat aacacgacca tgccgtatat
     3601 aacattattt ttcttagcgc cggataaaga aacaagtcct acattttgcg aataggctgt
     3661 atacggaaat gaattgaata tagaacctaa cactatcgct agaccttccg cagtataacc
     3721 tttacgaaaa tcttttcttt ctaacttctt accggtaatt tcacttaacg catgatagac
     3781 acctgtcgac tcaattaaac taacgatagc taaaataaag aacactaacg tcgatgtcac
     3841 atcaaagcta aatcctgaga atctaaacgg cactgggatg cctaaccaac cggcatgatt
     3901 gacttgatta atatcgacca tcccaagtaa gccagcacct atcgttccta aaacgagtcc
     3961 aattaatatg gcaatactct taataaatcc agttgtgaat ctttgtaaaa caagaataat
     4021 gattaatgtc attaaaccta acaaaatgtt cttaacatct ccatagtcct ttgcaccttg
     4081 acctccagct aagtaattca ttgctactgg cattaaattg ataccaatga tagtaacaac
     4141 actacccgtt actactggtg ggaagaattt tacaagatgt gaaaagaaag gcgcgatgat
     4201 aataactaat atccctgata aaaatagcga accgtaaagt acatctattc ctttcgtttg
     4261 accaattaaa atcatgggcg caacagccgt gaatgtacat ccaagaacga ttggtaatcc
     4321 tgttcctgtt actttattgg cttgtaaaaa tgtggcaacc ccacacataa atatatctac
     4381 tgtaactaag taagcgattt gttcaggtgt aaacttcaaa cttgtaccaa caatgattgg
     4441 aactaagatg gcacctgcgt acatagctaa aagatgttga acacttagga ttaaattttt
     4501 cattattctt ctcccaccaa tgtcactttg tttccttcta gtgaagcaac cttgcagaga
     4561 gaagaaactg ttaaacctgc ttcttctaaa cgttgatgcc cattttggaa actcttttca
     4621 acaacaatac caataccagc tgtcttagca ttcgcttgct gtgcgatatc gtataatcct
     4681 aatgaagcat caccatttgc taaaaagtca tcgatgatga gtacagtatc ttcttctgat
     4741 aaaaattctt ttgaaacaat gaccgtactt gttttatttt tagtaaatga atgaatagat
     4801 gtttcataat aaccatccgt caaagtgcta ggttttgctt ttttcgcaaa taaacatggc
     4861 acatcaaaat gcagtgcagc catgattgca ggtgcgatac cggaagcttc aatggttaag
     4921 attttagtaa tccctttatc tttaaattgc tcgtaaaaag tgcgaccaac ttcattcatt
     4981 aactttgcat caatttgatg atttaaaaat ccatcgactt ttaaaatctt ctcatcaatg
     5041 acaacgccgt cttcctttac tttttgtcct agtaactcca ctcttaaaac ctcctcagat
     5101 ttgtgtgaaa cattttgctc ttaaattggt gctagataca aaaaaatccc caaactaaat
     5161 aatagtttca gggtttatga gtgaacgaac atgcataacg aatttgtcat gcaatcaatg
     5221 taaaagaagt ttcatcaaat aaactgtgac catcatataa aatgatataa atcacccacc
     5281 atggttacaa tttaatggct gaagctactc ctagtattgt gttgttactc atagtcatgt
     5341 cgttcaaggc aacatggtag aaacttctaa agccatattc tttagattat atgagtttat
     5401 gtaaattatt taacgataat agcaaatttt cggcattttt tcaataactg cttaggtaat
     5461 cttttaatag ttttataaat gtacagagac gtttaatgcg aatgaagact tataacttaa
     5521 taatgtaagg gttttacttt ataataagaa gggtaaaaga ttaaataaac aaatttttaa
     5581 aggggcgaca actatggcag atattactgt agtaaatgac actggcgaat tatacaacgt
     5641 aatcaatcaa aagaaatctg aaggttattt agaatcagag ctaacaatta ttagtaaaag
     5701 taaattacac ctaaatgact tacacgactc tgaaatctcc cttatctcaa caagtggaac
     5761 atttagtgat agaatgacta aactgttaac aggagaagat ggtgaacatg cagttttatc
     5821 acgctacaac ttagcgcctg atgaattaga aaaatataaa caacttattt tagacgataa
     5881 aatgttagtt gtagcggttc gtgacaaatc atcacataaa gaagttcaag aacataactc
     5941 agcatatgaa gaaattgata ttactcattt tgctgaagca tctaaaggtc ccaaagctta
     6001 gttatcacac tgaattcaaa atgagattac tgtactgtct aatttaagat agtacagttt
     6061 tttataccat ttaaaagttt atgaacaatc ttttacagca ggcgattatg ctataatatt
     6121 atttaaattg gggaggtgtt cttatggaat ttaaaaccat tcaatctgtc aaagatccac
     6181 attacaacga agcacttaaa ctttacgata ctaagttaaa tttatcttta actgaagata
     6241 aacgtatcat caaacgctca ttagaaaaca acaaaaccga aaacgattat gtctttatcg
     6301 taggcttaga aaacgatgtt gttgtcagtc tagcaactgc acattatgaa gccacaacca
     6361 attcagcatt tttaatttat ttattcgcaa aagacctacc caaccatgat gagttaatga
     6421 cacaaacatt acatcaaatt gaacatgaac taaacttatt atcaaacctt gttcattcta
     6481 gaagtattaa ctttttaatg atggaagtcc ctaaagaacc tgcagattta acacaagata
     6541 acggacttat tattgaacac cgtagacagt ttttatacga acatcaattt gaaagacaaa
     6601 ctgaaattga ttacattcat ccaactaatg atggtcacac taaaaaagtg gacttatttt
     6661 taaagaaaaa tattaaactg acaaaggata tctacggtac aagtgttaaa tcaaattata
     6721 ttttgaagta tgtatttgcc aatggtatcg cacgtgaaat tatttatgct ttattagaag
     6781 aaatggattt acgtaaacca ttttaaacaa acttgaaatc actaacaata acgtttaaac
     6841 tatgtatgaa gcaagactga cgtttcactg cccgtagaca aacgtcagta tacataaagt
     6901 atatgcagaa aggactttaa agtatgttaa ctaaagaatt tgcacaacgc gtagaactaa
     6961 gtgagaagca agtccgtaaa attgttcaac atttagaaga acgtggttac caactaagca
     7021 agactgaata tcgtggtcgt gaagcaactg atttcaaaga agaagatatc gagcttttca
     7081 aagacattgc cgacaaagta aaacaaacaa atagttatga tctagcgttt gatgaattag
     7141 aaaaagaaaa agacttcctg caagtcattg tcaaaaacga tgacaaaaac ttacctacta
     7201 atcaaaatgt cgctcaacta gtagaagatt tacgcctaga aatccagaaa atgcgcgaag
     7261 aacgtcacct acttggtcaa atgatgaatc aagtacatca gcaacaacaa gaattaaaag
     7321 aacttcaaaa tcaacttaca tctaaaatcg attcaaatag cgaatcctta aaagccatcc
     7381 aaacatcaca agaggctatc caagaagcac aagcatctca agctaaagta ttggctgaat
     7441 caacaaataa agttgaaaag aatgctgtta ctgaagataa agctgacagt aaagattcga
     7501 aggttgctgg agtgaataca tcaacagacg ctaagacgga tactaaagca gaaaatgcag
     7561 gcgatggcac tgctactaaa gtagataaag aggatcaaat ttctgcaact gaagcaattg
     7621 aaaaagccag cgttgagcag tctaaaaatg aaaatgcagc tgagacttca aataaagaag
     7681 ctacagtaga tgcagacgcg caacatgatg ctgaacaaca agtagcagaa gcccatgcag
     7741 aagcttctaa acaagctact tcgaacgatt cattggaagc gaaagcagaa aatgacagca
     7801 cagcttcaca gtcagaaatg tcagaaccaa aacctcaaga agagaaaaaa ggtttcttcg
     7861 cacgtttatt caacctgtaa acttgtgatt tgaacacaaa tttaatactc aattcatata
     7921 tagctttgat ttaaaaactg attttttata aacctataga actgtaattt actttcaaat
     7981 ttaatactca attcataaaa cctgaaacaa gctgatatac tccattcaat atctactttg
     8041 aaga
//
LOCUS       DANBWH010000124         8032 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.128, whole genome shotgun sequence.
ACCESSION   DANBWH010000124 DANBWH010000000
VERSION     DANBWH010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8032
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.128"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(239..634)
                     /locus_tag="PPY92_001861"
     CDS             complement(239..634)
                     /locus_tag="PPY92_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent arsenate reductase"
                     /protein_id="HDJ2625850.1"
                     /translation="MDKKTIYFICTGNSCRSQMAEGWGKEILGEGWNVYSAGIETHGV
                     NPKAIEAMKEVDIDISNHTSDLIDNDILKQSDLVVTLCSDADNNCPILPPNVKKEHWG
                     FDDPAGKEWSEFQRVRDEIKLAIEKFKLR"
     gene            complement(652..1944)
                     /gene="arsB"
                     /locus_tag="PPY92_001862"
     CDS             complement(652..1944)
                     /gene="arsB"
                     /locus_tag="PPY92_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter membrane subunit
                     ArsB"
                     /protein_id="HDJ2625851.1"
                     /translation="MMTILAIVIFLLTLTFVIWQPKGLDIGITALIGAVVAIITGVVS
                     LSDVLEVTGIVWNATLTFVAVILISLILDEIGFFEWSAIHMVKASNGNGLKMFVFIML
                     LGAIVAAFFANDGAALILTPIVLAMVRNLGFNQKVIFPFIIASGFIADTTSLPLIVSN
                     LVNIVSADYFDIGFIEYFSRMIIPNIFSLIASILVLWLYFRKSIPKTFDTENLSDPKS
                     AIKDSKLFKLSWIVLAVLLVGYLVSEFIQIPVSIIAGIIAFIFVILARKSKAVHTKQV
                     IKGAPWNIVVFSIGMYLVVFGLKNVGITTVLGDVLTNISNYGLFSSIMGMGFIAAFLS
                     SIMNNMPTVLIDAIAIGQSSATGILKEGMVYANVIGSDLGPKITPIGSLATLLWLHVL
                     TQKGVKISWGTYFKTGIIITIPVLFVTLLGLYLTLIIF"
     gene            complement(1941..2255)
                     /gene="arsR"
                     /locus_tag="PPY92_001863"
     CDS             complement(1941..2255)
                     /gene="arsR"
                     /locus_tag="PPY92_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HDJ2625852.1"
                     /translation="MSYKELSTILKILSDPSRLEILDLLSCGELCACDLLEHFQFSQP
                     TLSHHMKSLVDNELVTTRKDGNKHWYQLNHAILDDIIQNLNIINTSNQRCVCKNVKSG
                     DC"
     gene            complement(2316..3074)
                     /locus_tag="PPY92_001864"
     CDS             complement(2316..3074)
                     /locus_tag="PPY92_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDJ2625853.1"
                     /translation="MTKNQNFIETKEYKRFAEFCDACIKYQYIGICYGQPGVGKTLSS
                     RYYTNWDTIEKQVNHRGWEDLASKTTDDILSVDKIFYTAPAEKQSGKSIENRPVFQEV
                     LNFLRMGDRLIVESIDRLGRNYEEIIQTVNYLKDKEVQLMITSLPMMNEVIGNPLLDK
                     FMKDLIIQILAMISEQERNESKRRQAQGIQVAKEKGIYKGRPVLYSPNAKDPQKRLVY
                     YRVVELLEQGKSISTIAKEVGITRQTIYRIKNSK"
     gene            complement(3067..4509)
                     /locus_tag="PPY92_001865"
     CDS             complement(3067..4509)
                     /locus_tag="PPY92_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDJ2625854.1"
                     /translation="MKNKEKYLTNFSEAKRKKATQKYNIIKPFILGEQSLSSISKSKG
                     IALSTLYRWNKSYKEQGLKGLIYATKADKGTRKIEPKIIDEIERLALMNKRNSIATIH
                     RKTTNYCKENNFDIPSYKQIYSVIKAMPKSVIDFSHQGEKYYQNKYDLIQIRESSRPN
                     EIWQADHTLLDIFILDQKGNINRPWLTIIMDDYSRAIAGYFLSFDAPNAQNTALTLHQ
                     AIWNKNDTNWPVCGIPEKFYTDHGSDFTSHHMEQVAIDLKINLMFSKVGVPRGRGKIE
                     RFFQTVNQTFLEQLPGYINNNDTPSNLINFHNFEEKLRYFLIEDYNQKEHSTIHTTPI
                     SRWNSNHFFPNMPSSLEQLDLLLLEIPKSRKVHSDGIHFQGFRYSNTNLAAYVGEYVL
                     IRYNPNDMAEIRVFYRDEFLCTAICPEIADYSIDIKDIQHARSQRRKHLKQNIASPST
                     TELIKEEKNCGYSPQETTKNVKKLKRYRND"
     gene            complement(4481..5074)
                     /locus_tag="PPY92_001866"
     CDS             complement(4481..5074)
                     /locus_tag="PPY92_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDJ2625855.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNQKDLNLLKTLYDNG
                     TPIKTIAEQWHVSRTTIYRYLNKLEDKEDEKQGEVSN"
     gene            complement(5338..5718)
                     /gene="blaI"
                     /locus_tag="PPY92_001867"
     CDS             complement(5338..5718)
                     /gene="blaI"
                     /locus_tag="PPY92_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HDJ2625856.1"
                     /translation="MTNKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            complement(5708..7465)
                     /gene="blaR1"
                     /locus_tag="PPY92_001868"
     CDS             complement(5708..7465)
                     /gene="blaR1"
                     /locus_tag="PPY92_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HDJ2625857.1"
                     /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTML
                     AGLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNINTTKPIQEFTTDIHKFNWDSI
                     DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIV
                     IRKAEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFN
                     IFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTPFSN
                     KNILSHSFNGKKSLLKSRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQSL
                     TDYNYKKPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYK
                     IYLALFGLDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIP
                     KNYTAAQLKQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQ
                     LSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGK
                     NAELISEKILKGMGVLNDQ"
     gene            7572..>8032
                     /locus_tag="PPY92_001869"
     CDS             7572..>8032
                     /locus_tag="PPY92_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDJ2625858.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALD
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKGFCC"
BASE COUNT         2684 a         1297 c         1069 g         2982 t
ORIGIN      
        1 aaattaacta tcattaaaca acgatatgac aatgtataat aaatacaatt tcactaccta
       61 taattcaaca ttatgtaaac taaagtattt caaataatta actgtattaa tactctcatc
      121 atgattgcgt tataaacgtt cgttaatgag aaaaagagag ggcatattgt atatggcaca
      181 aattttgttc tttaaatggc aaaacacctc ttattaacta aataagaggt gtttatgatt
      241 atctcaattt aaacttttct atagctaatt taatctcgtc tctaacacgt tggaattctg
      301 accattcttt acctgctgga tcatcaaaac cccaatgctc ttttttaacg tttggtggta
      361 aaataggaca attattgtct gcatcactac ataacgttac gaccaaatct gattgtttta
      421 aaatatcatt atcaatcaag tctgacgtat ggtttgatat atcaatatct acttctttca
      481 tagcttctat tgctttagga ttaacaccat gtgtttcaat accagcagaa tagacattcc
      541 aaccttcacc caatatttcc tttccccaac cttcagccat ttggctacga caagagtttc
      601 ctgtacatat aaaataaatt gttttcttat ccataattaa agcccctttt cttaaaatat
      661 gattagtgta aggtataaac ctaagagtgt tacaaatagg actggaatag tgatgataat
      721 tccagtttta aagtatgttc cccacgaaat cttcacacct ttttgtgtta agacatgtag
      781 ccacaataat gttgctaaag aaccaattgg cgtaatttta ggtcctaaat cagaacctat
      841 gacattcgca taaaccatgc cttcttttaa tattcctgta gcactggatt ggccaatcgc
      901 tattgcatct attaaaacag ttggcatgtt gttcataatc gaagataaga aagcagctat
      961 aaagcccata cccatgatgc tgctaaataa cccataattt gaaatgtttg ttaaaacatc
     1021 cccaaggacc gttgtaatac ctacattttt tagtccaaac acaacaagat acataccaat
     1081 agagaataca acgatattcc atggtgcacc tttaatcact tgttttgtat gaactgcttt
     1141 agatttacga gctaatatta caaagataaa agcaataata ccagcaataa ttgatacagg
     1201 aatttggata aactcgctaa caagatatcc aacgagtagt actgctaata ctatccatga
     1261 aagcttaaat aacttagaat ctttaattgc acttttagga tctgatagat tttctgtatc
     1321 gaacgtttta ggtatggatt ttctgaaata taaccataaa acgagaatac tagcaatcag
     1381 agagaatata ttaggaatga tcattcggct aaaatattca ataaatccaa tatcgaagta
     1441 atctgcagaa acgatattaa ctaagttact tacaattaag ggaagtgatg tagtatcagc
     1501 aataaaacca ctggcaataa taaaggggaa aatcactttt tgattaaatc ctagattcct
     1561 taccatcgct aatacaatag gcgttaagat taaagctgca ccatcatttg cgaaaaatgc
     1621 tgctacaatt gccccaagta acataataaa aacaaacatt tttaagccat taccgtttga
     1681 agccttgacc atatgtatcg cagaccattc aaaaaaacca atttcatcta atattaatga
     1741 aataagaata acagccacaa aagttaaagt tgcattccaa acaatacctg ttacttctaa
     1801 tacatcagaa agacttacga ctcctgtaat gatagcaaca accgctccaa ttaaagctgt
     1861 aataccaata tctaaacctt ttggttgcca aatcacaaag gttaaagtta aaagaaaaat
     1921 tacaattgct aaaatagtca tcaacagtca cctgatttca catttttaca tacacatctt
     1981 tgattagatg tattaatgat gttcaagttt tggataatat catctaaaat agcatgatta
     2041 agttgatacc aatgtttatt gccatctttt cgtgttgtaa ctaattcatt atctactaat
     2101 gacttcatat gatgacttag tgtaggttgt gagaattgaa agtgttctaa taagtcacaa
     2161 gcgcatagct caccacaaga aagtaaatct aatatttcta acctacttgg atctgataaa
     2221 atttttaata ttgttgatag ttctttataa gacatgaata aacctcctag atattaaata
     2281 gattaacatc tatatagatt attgtctatg ttattttatt tactattttt tattctatat
     2341 atagtttgac gtgtaatacc aacttcttta gctatagtac ttatagattt accctgttca
     2401 agtaattcaa caactcggta ataaactaaa cgcttttgtg ggtctttagc attgggagaa
     2461 tacagaacag gtctcccttt atatatacct ttttctttgg cgacttgaat cccttgagct
     2521 tgtcgacgtt tactttcatt tctttcttgt tctgaaatca ttgctaaaat ttgaataatt
     2581 aaatctttca taaatttatc tagtagtgga tttccaatga cttcattcat cataggtaaa
     2641 ctcgttatca tgagctgaac ttctttatct tttaagtaat taactgtttg aattatttcc
     2701 tcgtaatttc ttcctagtct atcaatagat tcaacaatta aacgatctcc cattcttaaa
     2761 aaatttaata cttcttgaaa aacaggtctg ttttctatag atttacctga ttgtttttct
     2821 gctggtgcag tataaaatat tttatccacc gatagtatgt catcggtcgt tttactagct
     2881 aaatcttccc aacctctatg gttaacttgt ttttcaatag tatcccaatt tgtgtaatac
     2941 ctagacgata aagtttttcc gacaccaggt tgaccataac atataccaat atactgatat
     3001 ttaatacaag catcacaaaa ttcagcaaag cgtttatact ctttcgtttc aatgaaattt
     3061 tgatttttag tcattacgat acctctttaa ttttttgacg tttttagtcg tttcttgagg
     3121 tgaataacca caattttttt cttccttaat taattccgtg gtacttggac tagcaatatt
     3181 ttgttttaag tgttttcttc tctgactacg agcgtgctgt atgtctttta tatctatgga
     3241 gtaatcagct atttctggac atatagcagt acaaagaaat tcatctctat aaaacacacg
     3301 tatttcagcc atatcatttg ggttataacg aatcagtaca tattcaccta cataagctgc
     3361 tagattagta ttactgtatc gaaaaccttg aaaatgaatg ccatctgaat gaacttttct
     3421 agatttaggt atttccaaca ataataaatc aagttgttct aagctactcg gcatattagg
     3481 gaaaaagtga ttactattcc aacgactgat aggcgtggta tgaatagtac tatgttcttt
     3541 ttgattataa tcttcaatta aaaagtaacg taatttttct tcaaaattgt gaaaatttat
     3601 tagattacta ggagtatcat tattgtttat atatccaggc agttgttcta agaaagtttg
     3661 attaactgtc tgaaagaatc gttctatttt tccacgacca cgaggaacac caactttcga
     3721 aaacatcaag ttaattttca aatcaatagc gacttgttcc atatgatgtg aagtaaagtc
     3781 acttccatga tctgtgtaga atttttcggg tataccacac actggccaat tggtatcatt
     3841 tttattccat attgcttggt gtagtgttaa tgctgtgttt tgagcattgg gggcatcaaa
     3901 acttaagaaa tagcctgcaa ttgcacgact atagtcatcc ataataatag ttagccatgg
     3961 tctattgata ttaccttttt ggtctaatat aaagatatct agcaaagtat ggtctgcttg
     4021 ccatatttca ttgggtctcg aggattctct aatttgtatt aaatcgtact tattttgata
     4081 gtatttttca ccctgatgag agaaatcaat aacagactta ggcatcgctt taataacact
     4141 ataaatttgt ttataacttg gtatatcaaa attattttct ttacagtaat tagtggtttt
     4201 cctatgaatt gtggctatac tatttctttt attcataagc gcaaggcgtt caatttcatc
     4261 aattatcttt ggttctattt tacgtgttcc tttatcagct tttgtcgcat agattagtcc
     4321 tttaagtcct tgttctttat atgacttatt ccatctataa agggtgctta acgcaatccc
     4381 tttactttta gaaatacttg ataaagattg ttcgcctagg ataaaaggtt ttataatatt
     4441 gtacttttga gtagcttttt tacgtttggc ttcagaaaaa ttagttagat acttctcctt
     4501 gtttttcatc ttccttgtcc tccaatttat tgaggtaacg ataaattgtt gtacgtgaaa
     4561 catgccactg ttctgcaatt gtcttaattg gcgtgccatt atcataaagt gtcttaagta
     4621 agtttaaatc tttttgattt aatttttctg gtctgcctcc atatcggcct ctagcacgtg
     4681 ctgcaattcg gccagctgaa gaacgctcta aaattaaatt acgttcaaat tctgcaaatg
     4741 ctgcgaataa atgaaataat aattgtccag ttgaacttga tttatccatt gttatatttt
     4801 cttctaaact atggaaactt acacctcgtt cgttaagctc attaactaat gtgattaaat
     4861 cctccatatt acgtcccaat ctatctaatc tccaaacaac aatcgtatct cctgacctcg
     4921 caaattcaat tgctttatct aaaccaggtc ttttactttt tgaaccactc atatggtcat
     4981 taaatatctt ttcacaacca tatgtattta atcgatcctc ctgtaaattt aaattttgta
     5041 atccagttga aactcttgca taacctattt tcaaaataca aacctcctat atacatcttc
     5101 agtgtaccat aacttaatta caaggacttt tatgaacata gattttttgt tgggtttttg
     5161 aacacataat cctatgtaat tcttacttat ttagacataa aagaagagtg tccagaaaat
     5221 ggttattttc tggacactct catctttaat aatatatttt aaatgtcatt aacaaaacta
     5281 atttaataag agtcaagtat ggtttacata tgtattatgt ttgaaacata cctctaatta
     5341 ctttttacta atatcattta aaatgtctcg caattcttca atttctttgt tatttaattc
     5401 ttcatttttc gcaaaattca gcactaaact tttcatatcc cctccataca gtttattaag
     5461 aaaggtttta gcagttttca ttttaatatc gtcttcttta atatttgatg agtaaaaata
     5521 aatattttct gatttgtatc gttttataat ctctttttta tatagtcttg tgattaatgt
     5581 tctaatcgtt ttatcactaa cttctttata tttttgaatt tcaactacaa tttcattagc
     5641 tgatactgat tttttatccc atattatatt cataacatcc cattcagcca tagatatttc
     5701 aacttgctta ttggtcattt aaaacaccca ttccttttaa tattttttca cttattaatt
     5761 cagcattttt cccagacggg tttccatctg ataaatgtgt agcaaaataa tacttatcat
     5821 gatttgtaat tacgtaacct acaaaccacc cattattata cttcccgttt actatacctg
     5881 tacctgtttt cccatacagt tcatactttt catttttctt aatcaataat gaagaagata
     5941 attgattctt tgctttttta ctaaaatggt tattttgttc catcatattt ttaaaaacta
     6001 ttacttgttc aagattagat attttcaaac tatcttccat ccaatagctt ttataacttc
     6061 ccaaattttc attaccataa tttaattgct tgagttgagc cgcagtatag ttctttggta
     6121 tttgattact aatacgttcg aagtaccaat taactgaatt ttgcattgct gtatttaaat
     6181 cttgttcctt attccaagac tcgaaaagat aatgtttatg attccatgac atacgtgaat
     6241 ttttatcact tataatatga cggtcaagtc caaacagtgc taaataaatt ttgtaagttg
     6301 aatcaggaga atatcttttt ctactttctt tttcattata aatatagtat ttgtctttct
     6361 tcatgctgta cataacgaaa gatccactat ttgaaccaaa atttttactt tcatctaaaa
     6421 tttggtaatc gctttgtaag ggctttttat aattataatc agttaaggat tgccccatta
     6481 aaaactggct ttgaattatc atgataaaaa aagtaaaaat acaaataaaa attagaatta
     6541 attttgactg ctttttcaga tcggcttctt ttatattaat taatctactt ttgagtaatg
     6601 attttttacc attaaatgaa tggcttaata tatttttgtt ggaaaatggg gtttttaata
     6661 cggagtccat aatagattcc gcataagttt taaattcatt tttgttaata ttattaagga
     6721 caaatctatc ggcttctact tcattgtcat gaattatctt tctttttaca atatgtacta
     6781 atggattgta actcataata atagagaaga tattaaagat aattaaatgt aaagtatctc
     6841 tatttttagc atgagcatat tcatgcagta ttatatattt tagtctttta tcaattacac
     6901 ttttaaaata tgaactagga atcaaaataa tatatttccc ataccaaaaa gttattggag
     6961 attgaatagc ctctgctttt cgaatcacaa tatttttttt atattgatgg ttgaaaagta
     7021 tcgtatctat tttatttttt tcattttcgt ttagataaag tgactgtttc tttaaatatt
     7081 taagatataa taaggctttc aaaaatttaa aacttaaaat aataactaaa actatccaaa
     7141 taactgtgca gatattatca attgaatccc aattaaactt atggatatct gttgtgaact
     7201 cttgaatagg tttggtggta tttatgttat ggttcaaatc gtgtgactta ctttcaactg
     7261 tgggcgcttg attattcaca ttattaaatt ttataaaaga gaatttaatt gggataaaag
     7321 gaattaatcc agcaagcata gttagatacc aaactttata atttaaacta taattaaaat
     7381 agcgttttaa tatgtaccta aaaaatacta acaatagaaa tataaaacaa aagcttacta
     7441 cgctcattat tagtaattta gccattttga caccttcttt caaatattta taataaacaa
     7501 ttgacatcaa tattacaatt gtaatattat tgatttataa aaattacaac tgtaatatcg
     7561 gagggtttat tttgaaaaag ttaatatttt taattgtaat tgctttagtt ttaagtgcat
     7621 gtaattcaaa cagttcacat gccaaagagt taaatgattt agaaaaaaaa tataatgctc
     7681 atattggtgt ttatgcttta gatactaaaa gtggtaagga agtaaaattt aattcagata
     7741 agagatttgc ctatgcttcg acttcaaaag cgataaatag tgctattttg ttagaacaag
     7801 taccttataa taagttaaat aaaaaagtac atattaacaa agatgatata gttgcttatt
     7861 ctcctatttt agaaaaatat gtaggaaaag atatcacttt aaaagaactt attgaggctt
     7921 caatgacata tagtgataat acagcaaaca ataaaattat aaaagaaatc ggtggaatca
     7981 aaaaagttaa acaacgtcta aaagaactag gagataaagg gttctgttgc aa
//
LOCUS       DANBWH010000125         7927 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.129, whole genome shotgun sequence.
ACCESSION   DANBWH010000125 DANBWH010000000
VERSION     DANBWH010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7927
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.129"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(412..858)
                     /gene="perR"
                     /locus_tag="PPY92_001870"
     CDS             complement(412..858)
                     /gene="perR"
                     /locus_tag="PPY92_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HDJ2625859.1"
                     /translation="MSVEIESIEHELEESIASLRQAGIRITPQRQAILRYLISSHTHP
                     TADEIYQALSPDFPNISVATIYNNLRVFKDIGIVKELTYGDSSSRFDFNTHNHYHIIC
                     EQCGKIVDFQYPQLNEIERLAQHMTDFDVTHHRMEIYGVCKECQDK"
     gene            complement(955..1905)
                     /locus_tag="PPY92_001871"
     CDS             complement(955..1905)
                     /locus_tag="PPY92_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDJ2625860.1"
                     /translation="MKVVGLNRMREVETELQQRFSDLDFKFYKKASEIPESDLADLDI
                     LVGYDGGINEAFLQRCPNLKWIAWFATGVNTLPLDYIADHGILLTNGKGVQAKQLSEY
                     ILAFILDDYKKMKLSYDNQRQHIYDSKITGKRLSGQTVLFLGTGAIATRTAKLAKAFN
                     MNLIGLSKSGQNKDEFDEIYTIESLESTLPNADIIINALPETQETIHLLKKKHFELMK
                     DEALFINIGRGSIVKEALLIEVLKSKVIRHAYLDVFENEPLKPNHELYELENVTITAH
                     ITGNDYEAKYDLLDIFKNNLVNFLNKNGLIENEVDAKKGY"
     gene            complement(1911..2366)
                     /gene="bcp"
                     /locus_tag="PPY92_001872"
     CDS             complement(1911..2366)
                     /gene="bcp"
                     /locus_tag="PPY92_001872"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDJ2625861.1"
                     /translation="MLQKGEQFPIFKLENQDGTVITNDTLKGKKAIIYFYPRDNTPTC
                     TTEACDFRDNLEMFNDLDVAVYGISGDSKKKHQNFIEKHGLNFDLLVDEDFKLAKETG
                     VYQLKKSFGKESMGIVRTTFIIDEQGKVLDVIEKVKVKTQIEELKNILG"
     gene            2447..3736
                     /locus_tag="PPY92_001873"
     CDS             2447..3736
                     /locus_tag="PPY92_001873"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDJ2625862.1"
                     /translation="MNFSESERLQQLSNEYILGGVNSPSRSYKAVGGGAPVVMKEGHG
                     AYLYDVDGNKFIDYLQAYGPIIAGHAHPHITKAIQEQAAKGVLFGTPTELEIEFSKKL
                     RDAIPSLEKIRFVNSGTEAVMTTIRVARAYTKRNKIIKFAGSYHGHSDLVLVAAGSGP
                     SQLGSPDSAGVPESVAREVITVPFNDINAYKEAIEFWGDEIAAVLVEPIVGNFGMVMP
                     QPGFLEEVNEISHNNGTLVIYDEVITAFRFHYGAAQDLLGVIPDLTAFGKIVGGGLPI
                     GGYGGRQDIMEQVAPLGPAYQAGTMAGNPLSMKAGIALLEVLEQDGVYEKLDSLGQQL
                     EEGLLKLIEKHNITATINRIYGSLTLYFTDEKVTHYDQVEHSDGEAFGKFFKLMLNQG
                     INLAPSKFEAWFLTTEHTEEDIQQTLKAADYAFSQMK"
     gene            4029..5123
                     /locus_tag="PPY92_001874"
     CDS             4029..5123
                     /locus_tag="PPY92_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDJ2625863.1"
                     /translation="MRLGARIFKTGIAIILAMSIASLLPDDVGLKALAGVSAVVAMQP
                     SIYRSFKTVSDQALGNIIGAILSVTMVTIFSDNFIIMGVTVIVLIAILFKFNLAHVAT
                     LASVTALIIMGQHTGSFYITAFYRFVLVMIGVISSSLVNFVFLPPKFETKIYYNSLNI
                     SSDIFMWFKLVLNDTTEFNNIKQDSHNLKQRVEKLEKIYDYYSEERPITKKHIHQQNR
                     KKILFREVVQTTRQAYEVLNKLSRYQNDLYLLNNNFLLQIKLDLDSLTAFHEQILASL
                     SKKARYNVTHVDYELDNPQKKDLLSTFQHELINHPYQTEYSFANVMQIVAAIEEYRHH
                     LEHLDRIRISFFTYHRSDADIEIVEEDFDL"
     gene            complement(5213..5304)
                     /locus_tag="PPY92_001875"
                     /pseudo
     CDS             complement(5213..5304)
                     /locus_tag="PPY92_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500499.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5313..7049)
                     /locus_tag="PPY92_001876"
     CDS             complement(5313..7049)
                     /locus_tag="PPY92_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAV1866 family putative multidrug efflux ABC
                     transporter"
                     /protein_id="HDJ2625864.1"
                     /translation="MIKRYLQFVKPYKYRIFATIIVGIIKFGIPMLIPLLIKYAIDGV
                     INNHALTTDEKVHHLTIAIGIALFIFVIVRPPIEFIRQYLAQWTSNKILYDIRKKLYN
                     HLQALSARFYANNQVGQVISRVINDVEQTKDFILTGLMNIWLDCITIIIALSIMFFLD
                     VKLTLAALFIFPFYILTVYVFFGRLRKLTRERSQALAEVQGFLHERVQGISVVKSFAI
                     EDNEAKNFDKKNTNFLTRALKHTRWNAYSFAAINTVTDIGPIIVIGVGAYLAISGSIT
                     VGTLAAFVGYLELLFGPLRRLVASFTTLTQSFASMDRVFQLIDEDYDIKNGVGAQPIE
                     IKQGRIDIDHVSFQYNDNEAPILKDINLSIEKGETVAFVGMSGGGKSTLINLIPRFYD
                     VTSGQILIDGHNIKDFLTGSLRNQIGLVQQDNILFSDTVKENILLGRPTATDEEVVEA
                     AKMANAHDFIMNLPQGYDTEVGERGVKLSGGQKQRLSIARIFLNNPPILILDEATSAL
                     DLESESIIQEALDVLSKDRTTLIVAHRLSTITHADKIVVIENGHIVETGTHRELIAKQ
                     GAYEHLYSIQNL"
     gene            complement(7340..7882)
                     /locus_tag="PPY92_001877"
     CDS             complement(7340..7882)
                     /locus_tag="PPY92_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDJ2625865.1"
                     /translation="MVRESIPKEGENIKIQSYKHDGKIHRVWSETTILKGTDHVVIGG
                     NDHTLVTESDGRTWITREPAIVYFHSEYWFNVICMFREDGIYYYCNLSSPFVCDEEAL
                     KYIDYDLDIKVYPNGKYHLLDEDEYEQHMNQMNYPHDIDIILRRNVDILQQWIEQKKG
                     PFAPDFIKVWKERYKKIRQY"
BASE COUNT         2599 a         1349 c         1125 g         2854 t
ORIGIN      
        1 ttttatataa tatcaagtta ttctagtaga gtcaacataa aacaacactt atttttgttt
       61 aaaaattact gttgaaggga ttattttcta ttcgtaagta ctatatgtat ttaaagtgta
      121 attccttacg ctttttactt cagttttaca aacaaactta tactcacttt atggatagtt
      181 atttagtaga ccttcgtttg aatattttta tgtttattta taaagggtac tagttattag
      241 ttaataccac ttccgtagtt agaatattct tttcttcaat ttaaatattt ttcagaataa
      301 gcataaaaaa gaagttaaac aactttcatg tcgtttaact tcttaaatat tacacttact
      361 tatatgaagt aagctaacgc ttttttaatt tgtcatacta ccaaagttaa attatttatc
      421 ttggcattct ttacaaactc cataaatttc cattcgatga tgtgttacgt caaagtcagt
      481 catatgctga gctaatcttt caatttcatt taactgtgga tattgaaaat caacaatctt
      541 accacattgt tcacatataa tatgataatg attatgtgta ttaaagtcga atcgacttga
      601 tgagtctcca tatgttaatt cttttacaat tccaatatct ttaaacactc ttaagttatt
      661 atatattgtc gcaacactta tatttggaaa atcaggtgaa agtgcttgat aaatttcatc
      721 agctgttgga tgagtatgtg aagaaattaa ataacgtaat attgcttgtc tttgaggtgt
      781 aattcttatg cctgcttgtc gcaatgatgc aattgattct tctagttcat gttcaattga
      841 ttctatttca acactcatct atatcaccat ctttcttata taataattat tattacttac
      901 tataaatata actgtagtat tgcgcgcgtg tcaatattta catgatgatt tcatttaata
      961 gcctttttta gcatcaactt cattctcaat tagaccattc ttattgagaa aattaactag
     1021 attgttttta aaaatatcta ataagtcata ctttgcctca taatcattac cagttatatg
     1081 cgctgttata gttacatttt ccaattcata taattcatga ttaggtttca aaggttcatt
     1141 ttcaaacaca tctaaatatg catgtcgaat aactttactt tttaatactt ctattaagag
     1201 cgcttcttta actatgctac ctcgtcctat atttataaaa agtgcttcat ctttcattaa
     1261 ttcaaaatgt tttttcttta gtaaatgaat cgtttcttgc gtttctggta aagcatttat
     1321 aataatgtca gcatttggta atgtgctttc aagtgattcg atagtatata tctcgtcaaa
     1381 ctcatctttg ttctgacctg acttgctcag accaattaaa ttcatattaa aagcctttgc
     1441 taacttcgca gttctagtag caattgcacc tgtacctaaa aataaaactg tttgtcctga
     1501 taggcgttta ccagttattt tcgaatcata tatatgttgt ctttggttat catatgatag
     1561 tttcatcttt ttataatcat ctaaaatgaa agctaaaatg tattcagata attgtttagc
     1621 ttgaacacct tttccattag ttaaaagtat gccgtgatct gcaatataat ctaacggtaa
     1681 tgtatttaca cccgttgcaa accatgcaat ccattttaaa ttcgggcaac gttgtaaaaa
     1741 tgcctcattg ataccgccat cataaccaac taatatatct aaatcagcca agtcactctc
     1801 aggtatttct gatgcttttt tataaaattt aaaatctaaa tctgaaaagc gttgttgtaa
     1861 ttcagtttca acttcacgca tacgatttaa cccaacaact ttcatatatg tcaccccaaa
     1921 atgtttttaa gttcttctat ttgtgtttta accttaacct tctcgataac atctaatact
     1981 ttaccttgtt catctattat aaaagttgtt cttacaatac ccatactttc tttgccaaat
     2041 gattttttta actgatatac gcctgtttct ttagctaatt taaaatcttc atctactaat
     2101 aaatcgaaat tcaatccgtg tttctcaata aaattttggt gttttttctt tgaatcaccg
     2161 cttataccat atactgcaac atctaaatca ttgaacattt ctaaattgtc tctaaagtca
     2221 caagcttctg tggtacaagt aggtgtatta tctctaggat aaaaatatat aatcgccttt
     2281 ttacctttta atgtatcatt tgtaatgaca gttccgtctt gattttctaa tttaaatatt
     2341 ggaaattgtt ctcctttttg caacatataa tcaccttttt cttcattata ataattttat
     2401 gatacgatat tagatgaaaa tattaaatta aaagaggttg atataaatga attttagtga
     2461 aagtgaacgt ttacaacaac tttcaaacga atatattcta ggcggtgtca attccccttc
     2521 tcgttcttat aaagctgtag gaggcggtgc acctgttgtt atgaaagaag gacacggtgc
     2581 atatttatat gatgtcgatg gcaataaatt tattgattac cttcaagcat acggtccaat
     2641 tattgcgggg catgcacatc ctcatattac taaagcaatt caagaacaag cagccaaagg
     2701 tgttttattt ggtacaccga ctgaactaga aattgaattc agcaaaaaat tacgtgatgc
     2761 aattccatct cttgagaaaa ttcgctttgt aaattctgga acagaagcag tcatgacaac
     2821 aattcgtgtt gcacgtgcat atactaaaag aaataaaatt ataaaatttg ctggatctta
     2881 tcatggccat tctgatttag tattggttgc agcaggtagt ggcccatctc agctcggttc
     2941 tccagactca gctggtgttc cagaaagcgt cgcacgtgaa gtcattactg tacctttcaa
     3001 tgatattaac gcatataaag aagcaattga attttggggt gatgaaattg ccgcagtatt
     3061 agtagaacca attgttggta actttggaat ggtaatgcct caacctggat ttttagaaga
     3121 ggttaatgaa atttcgcata acaatgggac actagtgatt tatgatgaag taattactgc
     3181 attccgtttc cattacggtg ccgctcaaga tttattaggt gttatccctg atttaactgc
     3241 atttggtaaa attgttggcg gtggtttacc aattggaggc tatggtggac gtcaagatat
     3301 tatggaacaa gtagcacctc taggacctgc atatcaagct ggtacaatgg ctggtaaccc
     3361 gttatctatg aaagcaggta ttgcattact cgaagtacta gaacaagacg gtgtttatga
     3421 aaaattagac agcttaggcc aacaactaga agaaggttta cttaaattaa tcgaaaaaca
     3481 taatatcaca gctacaatta atcgtattta tggatcttta acattgtact ttacagatga
     3541 aaaagtcaca cattatgatc aagttgaaca ttctgacggc gaagcgttcg gtaaattttt
     3601 caaattaatg ttaaatcaag gtatcaattt agcaccttct aagtttgaag cttggttctt
     3661 aacaactgaa catacagaag aagatattca acaaacttta aaagctgcag actatgcttt
     3721 tagtcaaatg aaataacttt taaggaagta tatttttaat tgaattaaaa taaaacaaat
     3781 gctcacatag tagattcatt ttatatcgtt aactattaac gtttaacttg atatgtcact
     3841 atgtgagttt ttattttaca aatttatact cttttaatct ctgactaaag ttttacatcg
     3901 ctatcagtat tatgtatgat ttattttaac aactataaat aagcttgaat tttataacta
     3961 gtaagtgtat aacagtaatg aatacgaata ttagatattc aataaactta aaaggggcgt
     4021 cattattttt gagactagga gctcggattt ttaaaactgg tatagccatt attttagcta
     4081 tgtctatcgc ttctttacta ccggatgatg ttggtctgaa agccttagct ggtgtcagtg
     4141 ctgttgttgc aatgcaacct agtatttatc gttcatttaa aacagtttct gatcaagcat
     4201 tgggtaacat tataggtgcc atattatctg ttacaatggt aacaattttt agtgataatt
     4261 ttatcattat gggtgttacc gtcatagtac ttattgctat tttatttaaa tttaatttag
     4321 cacatgttgc tactttggcg agtgtaacag cattaatcat tatgggacaa cataccggtt
     4381 ctttctatat taccgccttt tacaggtttg tacttgtaat gattggtgtc ataagttcat
     4441 ctctagttaa ctttgtgttt ctaccaccta aatttgaaac aaaaatttac tataattcat
     4501 taaatatatc ctcagatatt tttatgtggt ttaaattagt attaaatgat acgacggagt
     4561 ttaataatat taaacaagat agtcataatt taaaacaacg tgtcgaaaag ttagagaaaa
     4621 tttatgatta ctatagcgaa gaacgtccta ttacaaaaaa acatattcac caacagaata
     4681 gaaagaaaat acttttcaga gaagtagttc agacgactag acaagcttat gaagtattaa
     4741 acaaattgtc gcgttatcaa aacgacttat atttacttaa taacaatttc ctattacaaa
     4801 tcaaattaga tttagattca ttaactgctt ttcacgagca aattttagct agcctttcta
     4861 aaaaagcacg ttataatgtg acacatgttg attatgagtt agataatccg cagaaaaaag
     4921 atttattgtc tacatttcag catgagttaa taaatcatcc ttatcaaacg gaatattcgt
     4981 ttgccaatgt tatgcaaatt gttgcagcca tcgaagagta tcgacaccat ttagagcatt
     5041 tagaccgcat aagaattagc tttttcacat atcataggtc tgatgcagac attgaaattg
     5101 ttgaagaaga ctttgattta taagtttaaa gcaattcctg taattaatac cacaatgaga
     5161 taaagtaata ttgaatctcg aaattaaatt taaaaaagca gtaagattat tttcaatgaa
     5221 gaaaatctct tactgctttt ctatgttatg accccaccat cctgttacaa atcttcccta
     5281 caagattgta ctgaagtcaa acaactaata ctttataagt tttgaatgct atataaatgc
     5341 tcgtaagcac cttgttttgc aatcaattca cgatgcgtac ctgtttcaac aatatgtcca
     5401 ttttcaatta cgacaatttt gtcagcatgt gtaatagtgg acaagcgatg cgctacgata
     5461 agtgtcgttc gatctttact caacacatct aatgcttctt gaataatgga ttcactttct
     5521 aaatcaagtg cacttgttgc ttcatccaag ataagaattg gcggattatt taaaaatatt
     5581 ctagcaatcg ataatctttg tttttgacca cctgataatt taacacctcg ttcacctact
     5641 tcagtgtcat atccctgtgg caagttcata ataaagtcat gtgcattagc cattttcgcc
     5701 gcttcaacta cttcttcatc tgttgctgtt ggacgaccaa gtaaaatatt ttccttaact
     5761 gtatcagaga ataaaatatt atcctgttgc accaatccta tttgatttct taaacttccc
     5821 gttaaaaaat ctttaatatt gtgaccatct attaaaattt gcccagaagt tacatcgtaa
     5881 aatctcggta ttaagttaat taatgttgat ttaccaccac cactcatacc tacgaaagca
     5941 actgtttctc ctttttcaat actcaaatta atatctttta aaattggagc ttcgttatcg
     6001 ttatattgaa aactaacatg atcaatatca atacgacctt gtttaatttc aataggttga
     6061 gcaccaacac catttttaat gtcataatct tcgtcaatta attggaatac acggtccatt
     6121 gaagcaaaac tttgcgttaa agttgtaaat gatgcgacta aacgacgtaa aggcccgaac
     6181 aataactcta agtatccaac aaatgctgca agtgtaccta ctgtgattga tccagaaata
     6241 gcaagatatg caccaacacc gatgacaata attggtccaa tatctgtaac tgtattaatt
     6301 gcggcaaagg aataggcatt ccatcttgta tgtttcaacg cacgtgttaa gaaattagta
     6361 ttctttttat caaagttttt cgcttcattg tcttcaatcg caaaactttt aacgactgaa
     6421 ataccttgaa cacgttcatg caagaatcct tgaacctcag ctaatgcttg cgatctctca
     6481 cgcgtcaatt ttcttaatct tccaaagaaa acgtacaccg ttaaaatgta aaatgggaag
     6541 ataaacagtg ctgctaaagt caatttcaca tctaaaaaga acattatgga tagtgcaata
     6601 ataattgtta tacaatctaa ccaaatattc attaacccgg ttaaaatgaa atcttttgtt
     6661 tgttcaacat cattaatcac tctagatatt acttgaccta cttgattatt agcataaaat
     6721 ctcgcactta aagcttgtaa atggttatat aactttttac gtatatcata caatatttta
     6781 ttacttgtcc attgcgccaa atattgacgt ataaattcaa ttggtggtct cactattaca
     6841 aaaataaata atgcgatacc aatggcaata gttaaatgat gaactttttc atcagtcgtt
     6901 agtgcgtggt tattaatcac gccatctatt gcatatttaa ttaaaagtgg tataagcatt
     6961 ggtataccaa acttaattat cccaacaata atcgttgcaa aaatacgata tttatatggc
     7021 ttaacaaatt gcaaatatcg tttaatcata caatcccccc taatctattg ccctatccta
     7081 ttcataagca taaaaatgaa tagaggttgg atacataatt tgtagatgta aattcttctt
     7141 acaatttaca tttttaaaat taagatatct caacctctgt catattcatg tgattaaata
     7201 cgttgtctta ttcgtatgtt atattctatt caatttaatc tatggatact gtgtccccac
     7261 acgacagcaa aagttatcat acttctttac atcactaagt caatataaat gatttaatca
     7321 gtatttacac tttatttgct taatactgtc taattttttt gtaacgttct ttccaaactt
     7381 tgataaaatc tggcgcgaat gggcccttct tctgttctat ccattgttga agaatgtcca
     7441 cgttgcgtct taaaataata tcaatatcat gcggataatt catttgattc atatgttgct
     7501 catattcatc ttcatctaat aaatgatact ttccgtttgg atatacttta atatctaaat
     7561 catagtctat atattttaat gcctcttcat cacaaacaaa tggtgatgac aaattgcaat
     7621 agtaataaat tccatcttct ctaaacatgc agataacatt aaaccaatat tctgagtgaa
     7681 agtaaacaat tgccggttca cgtgttatcc aagttcttcc gtcactttca gtcactaacg
     7741 tatgatcatt gccaccaatg acaacatgat cagtaccctt taatattgtt gtttcagacc
     7801 aaacgcgatg aatcttacca tcatgtttat aactctgaat tttaatgttt tccccttctt
     7861 taggtatgga ttctctgacc atactccaca ccaccttctg ttaatttaac cattataaat
     7921 tatagca
//
LOCUS       DANBWH010000126         7799 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.130, whole genome shotgun sequence.
ACCESSION   DANBWH010000126 DANBWH010000000
VERSION     DANBWH010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7799
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.130"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            139..1281
                     /gene="bshA"
                     /locus_tag="PPY92_001878"
     CDS             139..1281
                     /gene="bshA"
                     /locus_tag="PPY92_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HDJ2625866.1"
                     /translation="MKIGITCYPSMGGSGIIATELGIKLAERGHEVHFITSNIPFRIR
                     KPLPNMIFHQVEVNQYAVFQYPPYDITLSTKIAEVIKEYDLDLLHMHYAVPHAICGIL
                     AREMSGKDIKIMTTLHGTDITVLGYDHSLQGAIKFGIEKSDIVTSVSKSLAQETHEII
                     ETNKEIIPIYNFVRENEFPTKHNTALKSQFGIAPDEKVLIHVSNFRQVKRIDTIIETF
                     AKVREKIPSKLILLGDGPELVPMRQLTKELNVEEDVLFLGKQDCVSEFYQLSDLVLLL
                     SEKESFGLTLLEAMKTGVVPIGSNAGGIKEVIKHGETGFVVDVGDCDSASDYAIRLLE
                     DKALYNKLQKNMLADIAERFGSELITDQYEYYYQKMLNEHNKSKGE"
     gene            1286..2488
                     /locus_tag="PPY92_001879"
     CDS             1286..2488
                     /locus_tag="PPY92_001879"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDJ2625867.1"
                     /translation="MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDI
                     TTSATPDEIESIFSHTIPVGKEHGTINVVFNDENYEVTTFRAEEDYVDHRRPSGVTFV
                     RDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMI
                     RCLRFQSQLSFDIAMETFEAMRTQMADIKFLSIERIVIELTKLMRGINVEESFNHLKS
                     LKAFNYMPYFEQLDMNQINVTEPIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQ
                     YIQIMNALPSIITKEQLKMFVYDYDTNLIKNVMVAADVLKANDIQGHEPLIVNLQTID
                     ETLHRLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILK
                     WVDNHVKI"
     gene            2475..3446
                     /locus_tag="PPY92_001880"
     CDS             2475..3446
                     /locus_tag="PPY92_001880"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDJ2625868.1"
                     /translation="MSKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLK
                     LEGCKIDSVNHKGHLLQQLPDIWYQGIIDQYTKSSALFDFSEVYDSIDSTQLAAKKSL
                     VGNQSSFFILSDEQTKGRGRFNRHWSSSKGQGLWMSVVLRPNVAFSMISKFNLFIALG
                     IRDAIQHFSQDEVKVKWPNDIYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLE
                     NFDESIRHRATSIQLHDKNKLDRYQFLERLLQEIEKRYNQFLTLPFSEIREEYIAASN
                     IWNRTLLFTENDKQFKGQAIDLDYDGYLIVRDEAGESHRLISADIDF"
     gene            3470..6163
                     /locus_tag="PPY92_001881"
     CDS             3470..6163
                     /locus_tag="PPY92_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase DinG"
                     /protein_id="HDJ2625869.1"
                     /translation="MGMATYAVVDLETTGNQLDFDDIIQIGITFVRNNQIIDTYHSMI
                     RTNLEIPPFIQALTSIEENMLQQAPYFNQVAQEIYDKIKDCIFVAHNVDFDLNFIKKA
                     FKDCNIQYRPKKVIDTLEIFKIAFPTDKSYQLSELAEAHGITLANAHRADEDAATTAK
                     LMILAFEKFEKLPLDTLKQLYYLSKQLKYDLYDIFFEMVRQYDAKPLDKSYEKFEQII
                     YRKQVDFKKPTTNYNGSLKSLYSKAVDQLGLTYRPQQLYLAETILDQLMHSEKAMIEA
                     SLGSGKSLAYLLAALMYNIETGKHVMISTNTKLLQSQLLEKDIPAMNEALNFKINALL
                     IKSKSDYISLGLISQILKDDTSNYEVNILKMQLLIWITETPSGDIQELNLKGGQKMYF
                     DQKIETYVPARHDVHYYNFIKRNAQNIQIGITNHAHLIHSDVENSIYQLFDDCIVDEA
                     HRLPDYALNQVTNELSYADIKYQLGLIGKNENEKLLKAIDQLEKQRILEKLDIAPIDI
                     FGLKASMNEIHELNEQLFSTIFTIINDSDVYDDDIHRFHNVFTFETKDILKDLHAIID
                     KLNKTLEIFNGISHKTVKSLRKQLLYLKDKFKNIEQSLKAGHTSFISIKNLSQKSTIR
                     LYVKDYAVKDVLTKQVLEKFKSLIFISGTLKFNHSFEAFKQLFNKDVHFNTFEVNTSL
                     QSAKNTSVFIPSDVASYQYKNIDEYVASIVSYIIEYTTITSSKCLVLFTSYKMMHMVQ
                     DMLNELPEFEDYVVLTQQQNQNYKIVQQFNNFDKAILLGTSTFFEGFDFQANGIKCVM
                     IAKLPFMNKHNAKYWLMDSEFTSTFKEYVLPDAVTRFRQGLGRLIRNENDRGIIVSFD
                     DRLINSNYKNFFEQTLENYRQKKGDIQQFGKLLRQIQKKKK"
     gene            6485..7777
                     /gene="asnS"
                     /locus_tag="PPY92_001882"
     CDS             6485..7777
                     /gene="asnS"
                     /locus_tag="PPY92_001882"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDJ2625870.1"
                     /translation="MKTTIKQAKDHLNQDVTIGAWLTNKRSSGKIAFLQLRDGTGFMQ
                     GVVVKSEVDEEVFKLAKEIAQESSLYVTGTITEDNRSDLGYEMQVKSIEVISEAHDYP
                     ITPKNHGTEFLMDHRHLWLRSKKQHAVMKIRNEVIRATYEFFNKDGFTKVDPPILTAS
                     APEGTSELFHTKYFDQDAFLSQSGQLYLEAAAMAHGKVFSFGPTFRAEKSKTRRHLIE
                     FWMIEGEMAFTNHAESLEIQEQYVTHVVKSVLENCKLELKILERDTSKLEKVATPFPR
                     ISYDDAIEFLKAEGFDDIEWGEDFGAPHETAIANHYDLPVFITNYPTKIKPFYMQPNP
                     ENEETVLCADLIAPEGYGEIIGGSERVDDLELLEQRVKEHGLDEEAYSYYLDLRRYGS
                     VPHCGFGLGLERTVAWISGVEHVRETAPFPRLLNRLYP"
BASE COUNT         2902 a         1040 c         1416 g         2441 t
ORIGIN      
        1 aaatgattat atatacaaca cctgtttttt agatatttga aattataaac tcaacattac
       61 tataaactta aatttaaaca attatctacg catcgagaat aatagaaaga gaaatagaaa
      121 gaaaggatgg gcaagcgaat gaagataggt ataacatgtt acccgtccat gggtggttct
      181 ggaattattg ccacagaatt aggaattaaa ttagcagagc gaggtcacga agtacatttt
      241 atcacttcaa atatcccgtt tagaataaga aaaccattgc cgaatatgat ttttcatcaa
      301 gttgaagtga atcaatatgc agtatttcaa tatccaccgt atgatattac gttaagtact
      361 aaaatcgctg aagttattaa agaatatgat cttgatttgt tgcatatgca ctatgctgta
      421 cctcatgcaa tttgtggaat tttagctcgt gagatgtcag gtaaagatat aaaaattatg
      481 acaacgctac acggtactga tattactgtt ttgggctatg atcattcact ccaaggtgca
      541 attaaatttg gcattgaaaa aagtgatatt gtgacaagtg tgagtaaatc tttagcgcaa
      601 gaaacacatg aaattatcga aactaacaaa gaaattatac cgatttataa ttttgtacgt
      661 gaaaatgagt ttccaactaa acataacaca gcattaaaat cacaatttgg tatagcacca
      721 gatgaaaaag tgttaataca tgtatcgaat tttagacaag taaaacgcat agatactatt
      781 atagaaacat ttgcaaaagt acgtgaaaag atacctagta aattaatttt acttggagat
      841 ggtcctgaat tagtgccaat gcgtcaattg acgaaagagt taaacgtaga agaggatgtt
      901 ttatttttag ggaaacaaga ttgtgtaagt gaattttatc aattatctga cttagtatta
      961 ttattaagtg aaaaagaaag ctttggacta actttacttg aggctatgaa aacaggcgtc
     1021 gttccaattg gttcaaatgc cggtggaatt aaagaggtta ttaaacatgg tgaaactgga
     1081 tttgttgtag atgtgggtga ttgtgattca gcgagtgact atgctatccg tttacttgaa
     1141 gataaggcgt tatacaacaa acttcagaaa aacatgctag cagatattgc tgaacgtttt
     1201 ggatcagaac ttattacaga tcaatatgag tattattatc aaaaaatgct aaatgagcat
     1261 aacaaaagta aaggcgagta ataatatgga taaatcatta tttgaacagg caaggcctat
     1321 attagaacaa attcaagaca atggttttga agcatattat gtaggtggct ctgtaagaga
     1381 ttatgtcatg ggaagaaata ttcatgatat agatatcaca acaagtgcaa cgccggatga
     1441 aatagaatct atctttagtc atacgatacc tgtaggtaaa gaacatggca ccataaatgt
     1501 agtttttaat gatgaaaatt atgaagtgac aacattccgg gctgaagaag attatgtcga
     1561 tcaccgtaga ccaagtggtg ttacatttgt tcgggattta tacgaagatt tgcaacgaag
     1621 agatttcacg atgaatgcga tagcaatgga tacagcatac aaattgtatg attattttga
     1681 tggtcaacaa gatattaata atcgaataat aagaactgta ggtatagctg aggaacgatt
     1741 ccaagaagat gctttacgta tgattcgatg tttaagattc cagtcacaat tatcatttga
     1801 tattgcaatg gaaacatttg aagcgatgcg tacacaaatg gcagatatta aatttttatc
     1861 aattgagcgt atagtgattg aactaactaa attaatgcga ggtattaatg ttgaagagag
     1921 ttttaatcat ttaaaatcgc tgaaagcatt taattatatg ccatatttcg aacaacttga
     1981 tatgaatcaa attaatgtaa ctgaaccaat tgatttagaa ttgttgattg ctatagtgtc
     2041 agttaaattt gatattaatt actcattgaa gcctttaaag ctaagtaacc gacaagtaaa
     2101 agatatcaat caatatattc aaattatgaa tgcattacca agtattatta caaaagaaca
     2161 attaaaaatg tttgtttatg attatgatac gaatctcatt aaaaatgtaa tggttgcagc
     2221 agacgtgtta aaggcaaatg atattcaagg acatgaacca ttaatcgtta accttcaaac
     2281 gattgatgaa acattacatc gtttacctat gcataataga aaagacatga tggttaatgg
     2341 cggtgtactt atggcacatt taaatgccaa aagtggtccg tggttaaaag atgtgctaag
     2401 acaaattgag atagcgattg taacaggtaa agtaagcaac gaagaaactg aaattttgaa
     2461 atgggtggat aatcatgtca aaatatagtc aagatgtact tcaattactc tataaaaata
     2521 aaccgaatta tatatctgga caaagcattg cggaatcact taatatttca cgcactgcag
     2581 taaaaaaggt gattgaccaa ttaaagttag agggatgtaa aattgattca gtaaatcata
     2641 aagggcattt attacaacaa ctcccagata tttggtatca gggtataata gaccaatata
     2701 caaaaagttc tgctttgttt gattttagtg aagtatacga ttcaatagat tctacacaac
     2761 ttgctgcgaa aaagtcactt gttggaaatc aatcttcatt ttttatcttg agtgatgaac
     2821 aaacgaaagg tcgtgggcga tttaatagac attggagttc ttcaaaaggg caaggacttt
     2881 ggatgtctgt cgtgttaaga cctaacgttg cattctcaat gatatctaaa tttaatttat
     2941 ttattgcatt agggataaga gatgcgattc aacattttag tcaagatgaa gtcaaagtga
     3001 aatggccgaa tgatatatat attgataatg gtaaagtgtg tggtttctta actgaaatgg
     3061 ttgctaataa tgatggtata gaagcaataa tatgtggtat aggtattaat ttgacgcaac
     3121 aactagaaaa ctttgatgaa agtattagac atagagcaac aagtatacaa ttacatgata
     3181 aaaataaatt agatagatat caatttttag agagattact tcaagaaatt gaaaaaagat
     3241 ataatcaatt tttaacgtta cctttttctg aaattcgtga agaatatatt gcagcttcta
     3301 atatttggaa tagaacgttg ctatttacag aaaatgataa acagtttaaa ggacaagcaa
     3361 ttgatttaga ttacgatggc tatctaattg ttagagatga agcgggtgaa tcacaccgtt
     3421 taattagtgc agatatagat ttttaacact aaagcaagga gagatagcta tgggtatggc
     3481 aacctatgcc gttgtggatt tggaaacaac aggcaaccaa ttagattttg acgatatcat
     3541 tcaaattggt ataacgtttg tgcgcaataa ccaaattatt gatacatatc attctatgat
     3601 tagaacaaat ttagagattc cgccgttcat tcaagcgtta acatccattg aagaaaatat
     3661 gctacaacaa gcgccatatt ttaatcaagt tgcacaagaa atctacgata agataaaaga
     3721 ttgcattttt gtcgctcata atgtagattt tgatttgaat tttattaaaa aggcatttaa
     3781 agattgtaat attcaatatc gtcccaaaaa agttatagat acactagaaa tctttaaaat
     3841 agcttttcct acagataaaa gctatcaatt aagtgaatta gcagaggcac atggtattac
     3901 cttagcaaat gcccatcgag ctgacgaaga tgccgctact actgctaaat tgatgatatt
     3961 agcttttgaa aaatttgaaa agttaccgtt agatactttg aagcaattat attatttaag
     4021 taaacagttg aaatacgatt tgtatgatat tttctttgag atggttaggc aatatgatgc
     4081 aaagccattg gataaatcgt acgaaaagtt cgaacagatt atctatcgta agcaagttga
     4141 ttttaaaaag ccgacaacaa attataatgg cagcttgaag tctttatata gcaaagctgt
     4201 agatcaactt ggcttaacat atcgaccgca acaattatat ttagcggaaa ctatattaga
     4261 tcagctcatg catagtgaaa aagcaatgat tgaagcatca ctaggcagtg gtaaatcatt
     4321 agcatattta cttgcggcct tgatgtataa tattgaaact gggaagcatg tcatgatttc
     4381 taccaatact aaattactgc aaagtcaatt gttagaaaaa gatattccag caatgaatga
     4441 agcgttaaat tttaaaatta atgcactatt gattaaaagt aaaagtgatt acatttcatt
     4501 gggacttatt agtcaaattt taaaagatga cacaagtaat tacgaagtga atatcttgaa
     4561 aatgcaattg ttaatttgga ttacagagac gccgtccgga gatatacagg aattaaattt
     4621 aaaaggtggt caaaagatgt attttgacca gaaaattgaa acatatgtac cggcacgtca
     4681 cgatgttcat tattataatt ttattaagcg aaatgcacag aacattcaaa ttggtattac
     4741 aaatcatgca catttaattc attctgatgt tgaaaattca atatatcaat tgtttgatga
     4801 ttgtatagtg gatgaagcac atcgcttacc agactatgcc ttaaatcaag tgacaaatga
     4861 gttgagttat gctgatatta aatatcagct tggtttgatt ggtaaaaacg aaaatgaaaa
     4921 attgttaaag gcaatcgatc aactggaaaa gcagcgaatt ttggaaaaat tagatattgc
     4981 accaattgat atttttggct tgaaggctag tatgaatgag attcatgagt taaatgagca
     5041 attgttctca acgatattta cgattatcaa tgattctgat gtttatgatg atgatattca
     5101 tcgcttccat aatgtattta cttttgaaac aaaagacatt ttgaaggatt tacatgcgat
     5161 tatcgataag ttaaataaaa cgctagaaat attcaatggc attagtcata aaacagttaa
     5221 atcattacgg aaacagttgt tgtatttaaa agataaattt aaaaatatcg aacaaagttt
     5281 aaaagctgga catactagtt tcatttcaat taaaaattta tctcaaaaat caacaatacg
     5341 tttatatgtg aaagattacg ctgtaaaaga tgtattaacg aaacaagtat tggaaaaatt
     5401 taaatcactc atttttatat ctggtacttt aaaatttaat cattcgtttg aagcttttaa
     5461 acagttattc aacaaagatg ttcactttaa tacatttgaa gttaacacgt cgttacaaag
     5521 tgcaaaaaat acaagtgtat ttataccgag tgatgtagca tcttatcaat ataaaaatat
     5581 agatgagtat gtagcatcga ttgtaagcta tattattgaa tatacgacta taacgtcatc
     5641 aaaatgttta gtcttattta cgagttataa aatgatgcat atggtacaag atatgctaaa
     5701 tgaattgcca gaatttgaag attatgttgt attaacacaa cagcagaacc aaaattataa
     5761 aatagtacaa cagtttaata attttgataa ggccatactg cttggtacgt caacattttt
     5821 tgaaggtttt gattttcaag caaatggtat taaatgtgtg atgatagcga agttaccgtt
     5881 tatgaacaag cataatgcaa aatattggct tatggattct gaattcactt caactttcaa
     5941 ggaatatgta ttacctgatg cagttacacg ttttagacaa ggacttggaa gattaattag
     6001 aaatgaaaat gatcgcggca taatagtttc atttgatgat cgactcataa acagtaatta
     6061 taaaaacttt tttgaacaaa cacttgaaaa ttatcgtcag aaaaaaggcg acattcaaca
     6121 gtttggaaaa ttattaagac aaattcaaaa aaagaaaaag tgaaatacaa agtaaaattc
     6181 ttgtgacatg acatgtgcta tgttctgtta caagaatttt tgtttggtcg tcaaaatttg
     6241 agttttataa ttaaacttga atgttagcac aaaaaacgac cttcatacta ataatattga
     6301 ggctttatta tatattttgt ctgaaactga tgattgtatt gtcttaatat aaattaatgt
     6361 tcaaatctgt gcgtaaatca ttaccattta cacgttatct tttagtctag ttctagaact
     6421 tgaaagcgga ttttgttaaa atgaatgagt attgtataac gaaaaaataa ataggagaat
     6481 ggttatgaaa acaacgatta aacaagcgaa agatcattta aaccaagacg ttacaattgg
     6541 tgcttggtta acaaataaac gttcaagtgg taaaatcgcc tttttacaat tacgtgatgg
     6601 aacaggcttt atgcaaggcg tagtagttaa atcagaagtt gatgaagagg tattcaaact
     6661 tgcgaaagaa attgctcaag aatcatctct atacgttaca ggcacaatta cagaagataa
     6721 tcgttctgac ttaggatacg aaatgcaagt gaaatcaatt gaagttattt cagaagcgca
     6781 tgactatccg attacaccta aaaatcatgg tacagaattc ttaatggatc accgtcattt
     6841 atggttacgt tctaaaaaac aacatgctgt aatgaaaatt agaaatgaag ttattcgtgc
     6901 aacgtatgaa tttttcaaca aagatggatt tacaaaggtt gatccaccaa ttttgacagc
     6961 aagtgcacca gaaggtacaa gtgaattatt ccatactaaa tactttgatc aagatgcgtt
     7021 tttatctcaa agtggtcagt tatacttaga agctgcagca atggcacacg gaaaagtatt
     7081 ttcatttggt ccaactttca gagctgaaaa atcaaaaaca cgtagacact tgatcgagtt
     7141 ctggatgatt gaaggggaaa tggctttcac aaatcatgct gaaagtttag aaattcaaga
     7201 acaatatgta acacatgtag taaaatcagt tttagaaaat tgtaaactag agttgaaaat
     7261 tttagagcgt gatacatcaa aacttgaaaa agttgcgaca ccattcccta gaatttcata
     7321 tgatgatgca attgaattct taaaagcaga aggctttgat gatattgaat ggggtgaaga
     7381 ttttggtgcg ccacatgaaa ctgccattgc taatcattat gatttaccgg tgtttattac
     7441 taattatcca actaaaatta agcctttcta tatgcaacca aatcctgaga atgaagaaac
     7501 tgtcttatgt gcagacttaa ttgcacctga aggatacggt gaaattatcg gtggatctga
     7561 acgtgtggat gacttagaat tgttagaaca acgcgttaaa gaacatggat tagacgaaga
     7621 agcatatagt tactacttag acttacgtcg ttatggtagt gtgccacact gtggatttgg
     7681 tttaggttta gagcgcacag tagcatggat ttctggtgtt gaacacgttc gtgaaactgc
     7741 gccattccca agattattaa accgtttata tccataagtt ttaggatcgt tacttaaaa
//
LOCUS       DANBWH010000127         7741 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.131, whole genome shotgun sequence.
ACCESSION   DANBWH010000127 DANBWH010000000
VERSION     DANBWH010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7741
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.131"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(315..674)
                     /locus_tag="PPY92_001883"
     CDS             complement(315..674)
                     /locus_tag="PPY92_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HDJ2625871.1"
                     /translation="MNIISTILIIFVALEFFYIMYLETIATTSKKTSETFNISVDKLK
                     DKNINLLLKNQGVYNGLIGVLLIYGLFISSNPKEICTAILVYIIGVAIYGGLSSNISI
                     FFKQGTLPVLALISMLW"
     gene            complement(693..1538)
                     /locus_tag="PPY92_001884"
     CDS             complement(693..1538)
                     /locus_tag="PPY92_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDJ2625872.1"
                     /translation="MNIMLTGATGHLGTHITNQAIANHIDHFHIGVRNVEKVPDDWRG
                     KVSVRQLDYFNQESMVEAFKGMDTVVFIPSIIHPSFKRIPEVENLVYAAKQSGVAHII
                     FIGYYADQHNNPFHMSPYFGYASRLLSTSGIDYTYVRMAMYMDPLKPYLPELMNMHKL
                     IYPAGDGRINYITRNDIARGVIAIIKNPDTWGKRYLLSGYSYDMKELAAILSEASGTE
                     IKYEPVSLETFAEMYDEPKGFGALLASMYHAGARGLLDQESNDFKQLVNDQPQTLQSF
                     LQENI"
     gene            2010..2690
                     /locus_tag="PPY92_001885"
     CDS             2010..2690
                     /locus_tag="PPY92_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL1"
                     /protein_id="HDJ2625873.1"
                     /translation="MKFKAIAKASLALGMLATGVITSNVQSVQAKTEVKQQSESELKH
                     YYNKPVLERKNVTGYKYTEKGKDYIDVIVDNQYSQISLVGSDKDKFKDGDNSNIDVFI
                     LREGDSRQATNYSIGGVTKTNSQPFIDYIHTPILEIKKGKEEPQSSLYQIYKEDISLK
                     ELDYRLRERAIKQHGLYSNGLKQGQITITMKDGKSHTIDLSQKLEKERMGDSIDGRQI
                     QKILVEMK"
     gene            2976..3671
                     /locus_tag="PPY92_001886"
     CDS             2976..3671
                     /locus_tag="PPY92_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL2"
                     /protein_id="HDJ2625874.1"
                     /translation="MKMKNIAKISLLLGILATGVNTTTEKPVHAEKKPIVISENSKKL
                     KAYYTQPSIEYKNVTGYISFIQPSIKFMNIIDGNSVNNLALIGKDKQHYHTGVHRNLN
                     IFYVNEDKRFEGAKYSIGGITSANDKAVDLIAEARVIKADHIGEYDYDFFPFKIDKEA
                     MSLKEIDFKLRKYLIDNYGLYGEMSTGKITVKKKYYGKYTFELDKKLQEDRMSDVINV
                     TDIDRIEIKVRKA"
     gene            3962..5032
                     /locus_tag="PPY92_001887"
     CDS             3962..5032
                     /locus_tag="PPY92_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL3"
                     /protein_id="HDJ2625875.1"
                     /translation="MNMKTIAKTSLALGLLTTGAITVTTQSVKAEKIQSTKVDKVPTL
                     KAERLAMINITAGANSATTQAANTRQERTPKLEKAPNTNEEKTSASKIEKISQPKQEE
                     QKSLNISATPAPKQEQSQTTTESTTPKTKVTTPPSTNTPQPMQSTKSDTPQSPTIKQA
                     QTDMTPKYEDLRAYYTKPSFEFEKQFGFLLKPWTTVRFMNVIPNRFIYKIALVGKDEK
                     KYKDGPYDNIDVFIVLEDNKYQLKKYSVGGITKTNSKKVDHKAELSVTKKDNQGMISR
                     DVSEYMITKEEISLKELDFKLRKQLIEKHNLYGNMGSGTIVIKMKNGGKYTFELHKKL
                     QEHRMADVIEGTNIDKIEVNIK"
     gene            5150..5287
                     /locus_tag="PPY92_001888"
     CDS             5150..5287
                     /locus_tag="PPY92_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625876.1"
                     /translation="MFVKVAFLCLKSDESSNVPSVESHQNQFYLTNIMDFLIYLTMIQ
                     I"
     gene            5396..6274
                     /locus_tag="PPY92_001889"
     CDS             5396..6274
                     /locus_tag="PPY92_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL4"
                     /protein_id="HDJ2625877.1"
                     /translation="MKITTIAKTSLALGLLTTGVITTTTQEANATTPSSTKVEAPQST
                     PPSTKVEAPQSKPNATTPPSTKVETPQQTPNATTPSSTKVETPQSPTTKQVPTEINPK
                     FKDLRAYYTKPSLEFKNEIGIILKKWTTIRFMNIVPDYFIYKIALVGKDDKKYDEGVH
                     RNVDVFVVLEEKNKYGVERYSVGGITKSNSKKVDHKAGVRITKEDNKGTISHDVSEFK
                     ITKEQISLKELDFKLRKQLIENHNLYGNVGSGKIVINMKNGGKYTFELHKKLQENRMA
                     DVIDGTNIDNIEVNIK"
     gene            6638..7342
                     /locus_tag="PPY92_001890"
     CDS             6638..7342
                     /locus_tag="PPY92_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL5"
                     /protein_id="HDJ2625878.1"
                     /translation="MKMAAIAKASLALGILATGTITSLHQTVNASEHEAKYENVTKDI
                     FDLRDYYSGASKELKNVTGYRYSKGGKHYLIFDKNRKFTRVQIFGKDIERFKARKNPG
                     LDIFVVKEAENRNGTVFSYGGVTKKNQDAYYDYINAPRFQIKRDEGDGIATYGRVHYI
                     YKEEISLKELDFKLRQYLIQNFDLYKKFPKDSKIKVIMKDGGYYTFELNKKLQTNRMS
                     DVIDGRNIEKIEANIR"
BASE COUNT         3016 a         1165 c         1309 g         2251 t
ORIGIN      
        1 atagatccat gagagtagga accccaccat acgggaattt cgaaatcatg ggttttttgt
       61 tttaaaggat tgatgcaata gataaagtag ccattctaat atatggagta tttatcagct
      121 aaaaattaaa acagatatgg gttttcttca ttaaatcatt tttctataag gaaacagagg
      181 aaacgctact gtcacctctg cttgaaataa ttaggatgaa atactctgag tttatgaacg
      241 tccacaccca aaacactcaa accctccaaa ctctcaaccc cagactacat ctccaaaccc
      301 ccaaacacca atacttacca aagcattgat atgagtgcca atactggcaa tgtgccttgt
      361 ttgaaaaaga tactaatatt gcttgaaagg ccaccataaa tagcaacgcc aatgatatac
      421 actaaaatag ctgtgcatat ttcttttgga ttactgctga taaacaaacc gtatattagc
      481 aaaactccga ttaaaccgtt atatacgcct tggttcttca aaagtaggtt aatatttttg
      541 tctttcaatt tatcgacgct tatattaaat gtctcgctag tctttttgga agttgtagca
      601 atcgtttcaa ggtacataat atagaaaaac tctaatgcca caaatatgat taaaattgtt
      661 gagatgatat tcactgtaac gctcctttat tattaaatat tttcttgtaa aaatgattgc
      721 agtgtctgtg gttgatcatt gactaattgt ttgaaatcat tggattcttg gtctaatagt
      781 cctcttgctc ctgcgtggta cattgatgcc aataatgcac caaagccttt aggttcatcg
      841 tacatttctg caaatgtctc taatgaaacg ggttcatatt taatttctgt gcctgatgcc
      901 tcagataaaa tagcagcaag ttctttcata tcataactat agcctgataa taaatagcgt
      961 ttgccccaag tatctggatt tttaataata gcaatgacac ctctagcaat atcatttcta
     1021 gtaatataat taatacgacc atcaccagct ggataaatca gtttatgcat attcatcaat
     1081 tctggtaaat agggtttaag tggatccatg tacattgcca ttcttacata cgtataatca
     1141 atgccacttg ttgacaataa acgtgatgca tatccgaaat aagggctcat atggaatgga
     1201 ttattatgct gatctgcgta ataacctatg aaaatgatat gagcaacgcc gctctgcttt
     1261 gccgcatata ctaaattttc cacttcagga atacgtttga atgatggatg gataatactt
     1321 ggaataaaca caacggtatc cattccttta aatgcttcta ccatgctttc ttgattaaaa
     1381 taatctaatt gtcgaacaga aacttttcca cgccaatcat ctggaacttt ctcaacattt
     1441 ctaacaccaa tgtgaaaatg atctatgtga tttgcaatgg cttgatttgt aatatgtgtg
     1501 cctaaatgac ctgtagcacc tgttaacata atattcattc acttcatctc ctaatcttta
     1561 tatacataac ataatactta ttcgattgtt ttcaaaacat ttgattttat aaaaaattgt
     1621 aatctgtatt tattatcgct gtgtatagta aatacgtata tgttattaat gttgaaaata
     1681 ccgtaatgac gcgttttagt tgatgtgtat cactaatatc tttgaaaatt ttaatcaggt
     1741 actacgacaa tatgatgtct gttttgtgtc tgaaagtttt acagttttta aaataaaaat
     1801 ggtataaagt gtgatttgta taaaaaagag tctcgacgga taagaattga ttaataacag
     1861 ttagcatttt attaattacc ttaacaatga ttcaagttta gttaaatgag gtttaatttg
     1921 aaaggggata gcgcctcaat ataatgtagg tagattgttc atattacgta attgaaaaat
     1981 caaatttaaa tagattgggg ctaaaaatta tgaaatttaa agcgatagca aaagcaagtt
     2041 tagcattggg aatgttagca acaggtgtaa ttacatcgaa tgtacaatca gtacaagcga
     2101 aaacagaagt taaacaacaa agtgaatcag agttgaaaca ctattataat aaaccggttt
     2161 tagagcgtaa aaatgttact ggatataaat atactgaaaa aggtaaagat tatatagatg
     2221 ttatagtaga caatcaatat tctcaaattt ctttagttgg atctgataaa gacaaattta
     2281 aagatggaga caactcgaat atagatgtgt ttatccttag agaaggtgac agtagacaag
     2341 caacaaatta ctcaattggt ggcgtaacaa aaacaaacag tcaacctttt attgactata
     2401 tacacacacc aatccttgaa atcaagaaag gtaaagaaga accacaaagt agtttatacc
     2461 aaatttataa agaagacatc tcattgaaag aacttgatta tagattaaga gaacgtgcaa
     2521 tcaaacaaca cggcttgtat tcaaatggtc ttaaacaagg tcaaattaca attacaatga
     2581 aagatggcaa atcacatact atcgatttaa gtcaaaaact tgaaaaagaa cgtatgggtg
     2641 attctatcga cggcagacaa atacaaaaaa ttctagtaga aatgaaataa tactttctaa
     2701 caacaaagcg ctatgttgaa tagtgcttgt tatggaaata tatggaagtt aagcgacgta
     2761 ctgttgctta gcttcttttt ttgaggggaa aagttacaaa actcacacaa acagtcgcac
     2821 cacgcattat cttttgctta aatagcttaa tcatatttta tgaatagtta aaaacaggtt
     2881 aatgtgaata tccgaataca gctcctataa tatgggtgta tggttcaaat tacgtaataa
     2941 aacaatctaa ttataataga ttggagcata caactatgaa aatgaaaaat attgcaaaaa
     3001 taagtttgtt attaggaata ttagcaacag gtgtaaacac tacaacggaa aaaccagttc
     3061 atgccgaaaa gaaacctatt gtaataagtg aaaatagcaa aaaattaaaa gcttattata
     3121 ctcaacctag tattgaatat aaaaatgtga caggttatat cagtttcatt caaccaagta
     3181 ttaaatttat gaatatcata gatggtaatt ctgttaataa ccttgcttta attggcaaag
     3241 ataagcaaca ttatcatacg ggtgtacatc gtaatcttaa tatattttac gttaatgagg
     3301 ataagagatt tgaaggtgca aagtactcta ttgggggtat cactagtgca aacgataaag
     3361 ctgtcgacct aatagcagaa gcaagagtta ttaaagcaga tcatattggt gaatatgatt
     3421 atgacttttt cccatttaaa atagataaag aagcgatgtc attgaaagag attgatttta
     3481 aattaagaaa ataccttatt gataattatg gtctttacgg tgaaatgagt acagggaaaa
     3541 ttaccgtcaa aaagaaatac tatggaaagt atacatttga attggataaa aagttacaag
     3601 aagaccgtat gtccgatgtt atcaatgtca cagatattga tagaattgaa atcaaagtta
     3661 gaaaagctta atacacatac ttgacgacga aataatttga aattgaaata gagaggttaa
     3721 gtgacgatca aacgttgctt aacttctttt taatgcttaa aaatcatttc aaaggcacat
     3781 agaaacgcta tattaacctc ataatcactc attatttttt gcttaaatta cttaataata
     3841 cttcaataat tgttaaaaag ggtttaatgt gattatctta gaacgccatc tataatgatg
     3901 ttgtatgatt caaattacgt aaaaagacaa tcgaatataa tatagattgg agtatacaat
     3961 tatgaatatg aaaacaattg ctaaaaccag tttagcacta gggcttttaa caacaggcgc
     4021 aattacagta acgacgcaat cggtcaaagc agaaaaaata caatcaacta aagttgacaa
     4081 agtaccaacg cttaaagcag agcgattagc aatgataaac ataacagcag gtgcaaattc
     4141 agcgacaaca caagcagcta acacaagaca agaacgcacg cctaaactcg aaaaggcacc
     4201 aaatactaat gaggaaaaaa cctcagcttc caaaatagaa aaaatatcac aacctaaaca
     4261 agaagagcag aaatcgctta atatatcagc aacgccagcg cctaaacaag aacaatcaca
     4321 aacgacaacc gaatccacaa cgccgaaaac taaagtgaca acacctccat caacaaacac
     4381 gccacaacca atgcaatcta ctaaatcaga cacaccacaa tctccaacca taaaacaagc
     4441 acaaacagat atgactccta aatatgaaga tttaagagcg tattacacga aaccgagttt
     4501 tgaatttgaa aagcagtttg gatttttgct caaaccatgg acgacggtta ggtttatgaa
     4561 tgttattcca aataggttca tctataaaat agctttagtt ggaaaagatg agaaaaaata
     4621 taaagatgga ccttacgata atatcgatgt atttatcgtt ttagaagaca ataaatatca
     4681 attaaaaaaa tattctgtcg gtggcatcac gaagactaat agtaaaaaag ttgatcacaa
     4741 agcagaatta agcgttacta aaaaagataa tcaaggtatg atttcacgcg atgtttcaga
     4801 atacatgatt actaaggaag agatttcctt gaaagagctt gattttaaat tgagaaaaca
     4861 acttattgaa aaacataatc tttacggtaa catgggttca ggaacaatcg ttattaaaat
     4921 gaaaaacggt gggaagtata cgtttgaatt acacaaaaaa ctgcaagagc atcgtatggc
     4981 agatgtcata gaaggtacaa acattgataa aattgaagtg aatataaaat aatcatgacg
     5041 ttctctaaat agaagctgac atcggtaaaa caagaagtta agtgacaacg gtttacatgt
     5101 tgcttagctt cttttattat gcgtaatgat gtaaaagacg aatattcatt tgtttgtaaa
     5161 agtggcattt ctatgtctta aaagtgacga atcctcaaat gtgccaagtg ttgaatcaca
     5221 tcaaaatcag ttttatttaa cgaacattat ggatttctta atttacttaa cgatgattca
     5281 aatatagtta aacaaggttt aatgtgaatg gagcaatacg ccatctataa taaagctgta
     5341 ggattcaatg aatgtaatcg aacaaatcta ataattacga atggagcata caactatgaa
     5401 aataacaaca attgctaaaa caagtttagc actaggcctt ttaacaacgg gtgtaatcac
     5461 aacgacaacg caagaagcaa acgcgacaac accatcttcc actaaagtgg aagcaccaca
     5521 atcaacaccg ccctcaacta aagtagaagc accgcaatca aaaccaaacg cgacaacacc
     5581 gccttcaact aaagtggaaa caccgcaaca aacaccgaac gcgacaacac catcttcaac
     5641 taaagtggaa acaccacaat cgccaaccac aaaacaagta ccaacagaaa taaatcctaa
     5701 atttaaagat ttaagagcgt attatacgaa accaagttta gaatttaaaa atgagattgg
     5761 tattatttta aaaaaatgga cgacaataag atttatgaat attgttccag attatttcat
     5821 atataaaata gctttagttg gaaaagacga taaaaaatat gatgaaggag tacataggaa
     5881 tgtcgatgta tttgtcgttt tagaagaaaa aaataaatat ggagtggaaa gatactcggt
     5941 cggtggtatc acaaagagta atagtaaaaa agttgatcac aaagcaggag taagaattac
     6001 taaagaagat aataaaggta caatctctca tgatgtttca gaattcaaga ttactaaaga
     6061 gcagatttcc ttgaaagaac ttgattttaa attgagaaaa caacttattg aaaatcataa
     6121 tctgtacggt aacgttggtt caggtaaaat tgttattaac atgaaaaacg gtggaaagta
     6181 cacgtttgaa ttacacaaaa aattacaaga aaatcgcatg gcagacgtca tagatggcac
     6241 taatattgat aacattgaag tgaatataaa ataatcatga cattctctaa atagaagctg
     6301 tcatcggaaa aacaagaagt tcagtgacaa cggcctacat gttgcttagc ttcttttgtt
     6361 atgttcgatg atttgagaac ccgaattttc gatgggtcca aatatgacgt ggaagagtcc
     6421 tgaatttatc tgtaaatccc tatctatcgg gtgtgaagca caacggaatc agttttattt
     6481 aacgaacatt atagattcct taatttactt aataatgatt caatgattat taaagatggt
     6541 ttaatgtgaa aggtcaaata cgccaattat aataaagctg tatgattcaa tagacgtaag
     6601 cgaacaaatc taataattac gaatggagca tacaactatg aaaatggcag caattgcgaa
     6661 agcaagttta gcattaggta ttttagcaac aggaacaata acgtcattgc atcaaactgt
     6721 aaatgcgagt gaacatgaag caaaatatga aaatgtgaca aaagatatct ttgacttaag
     6781 agattactat agtggcgcaa gtaaggaact taaaaatgtt actggttatc gttatagcaa
     6841 aggtggcaag cattacctta tctttgataa aaatagaaaa ttcacaagag tacagatatt
     6901 tggtaaagat attgaaagat ttaaagcacg caaaaatccg ggattagaca tatttgttgt
     6961 taaagaagca gaaaatcgca acggcacagt gttttcatat ggtggtgtca ctaagaaaaa
     7021 tcaagacgct tattatgatt atataaacgc accaagattt caaatcaaga gagatgaagg
     7081 tgacggtatt gctacgtacg gtagagtaca ctacatttat aaagaagaga tttcacttaa
     7141 agaactcgac tttaaattga gacagtattt aattcaaaat tttgatctgt ataaaaagtt
     7201 tcctaaagat agtaagataa aagtgataat gaaagatggc ggctattata cgtttgaact
     7261 taataaaaaa ttacaaacaa atcgcatgag tgatgtcatt gacggtagaa atattgaaaa
     7321 aatagaagcc aacattagat aattcaatga aatatggata atagtaaaat atggatagta
     7381 tagaggagtt aggcaacata agttgcttag cttctttttt gtgttggcga gatgaaaatg
     7441 aagcgtatcg atgaataata aaaacaccaa taaaacttgt ggaaatagtt gatacttata
     7501 gatgcgtgat gtcgctttag tgacatgaaa caatgtggaa aacataatta aattgaggga
     7561 aagtgtgaat agttaaaaaa ttagcattgt gttataaaaa ataattaata ctgttaggat
     7621 ttcattaact aacttaacgt tggttcaaaa atagttaaaa agaggttaat tcatagcgca
     7681 gtatctcgct tatataatga tagtagattg ttcgtattac gtaattgaat taatcatata
     7741 a
//
LOCUS       DANBWH010000128         7733 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.132, whole genome shotgun sequence.
ACCESSION   DANBWH010000128 DANBWH010000000
VERSION     DANBWH010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7733
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.132"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(67..384)
                     /locus_tag="PPY92_001891"
     CDS             complement(67..384)
                     /locus_tag="PPY92_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDJ2625879.1"
                     /translation="MKSMVEMQREVDEYIGQFKTGYFSPLANLARLTEEVGELAREIN
                     HTYGEKKKKDSEEANTIKAELGDNLFVLLCLANSMGIDMTESFNETMEKFNTRDKNRF
                     ERK"
     gene            complement(720..1400)
                     /locus_tag="PPY92_001892"
     CDS             complement(720..1400)
                     /locus_tag="PPY92_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDJ2625880.1"
                     /translation="MIIYFVILMVLPLWAQHKVKSNYEKYSQVRSTSGKTGREVALEI
                     LHANGIYDVEVVKGEGFLTDHYDPKKKVVSLSPANYDRPSVAGTAIAAHEVGHAIQDH
                     QGYWFLRFRAALVPVANLGSSLSYMIIMLGIILTAIGSAFGSTALWIGAGLMSLAVLF
                     SIVTLPVEFDASSRAMKQITALNIVNEKEYKHARKVLSAAAMTYVAATAVAVAELVRI
                     ILIARSSD"
     gene            complement(1472..2059)
                     /locus_tag="PPY92_001893"
     CDS             complement(1472..2059)
                     /locus_tag="PPY92_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1405 domain-containing protein"
                     /protein_id="HDJ2625881.1"
                     /translation="MTINTFWQYTLYQRSWLMMLLICNILGMIYGYIWYGEQLSHTPW
                     HFKIFVPDSPTAILYLVISISLILIQKQNSIIEALAFVTLFKYGIWAVIMNILFIIEQ
                     GDITVNGLVLMFSHSIMAVQAIYFYPRFKRSMIGISVAMIWVFLNDYIDYFHLQFPYY
                     DFITTHVWQIGVLSCCLSVFGLLLYIELNKLLKCK"
     gene            complement(2049..2624)
                     /locus_tag="PPY92_001894"
     CDS             complement(2049..2624)
                     /locus_tag="PPY92_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HDJ2625882.1"
                     /translation="MSETLNQIKESFIEYLLFQYRFKSRIAVWVLNYIKVNEAKLANI
                     HFVDTKINHHETLEIAEVGSHASAIQFTKRNIKLMNTNEIFDYIANHNCAFDIQIHFA
                     NVSKREQRLDDLIVAQLTESPSYQTYLHDLNSMAIDRHKHALLIDYLLHNIDLSLQMN
                     EKQRFYQLTQILNTLKLVNKHNQFEDLADDD"
     gene            complement(2638..3882)
                     /locus_tag="PPY92_001895"
     CDS             complement(2638..3882)
                     /locus_tag="PPY92_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625883.1"
                     /translation="MEDIYKLIDDINLQKLENLDSRVNEAITTDNDDALFILGETLYN
                     FGLMPQGLEVFRVLYHKYPDESELLIYFIEGLMSENQTDEALEYLSYVEPSPEKLMLE
                     ADLYQQINMMEVAIDKLQEALELEPNDPIIHFALAEMLYYDGQYLRATSEYETVLETG
                     EYQVNGVNLFSRMADCSLQSGNYSDAIRLYDEINEDEMTSEDYLKKAISYDKNDITQE
                     AIKIMTTLLSKDPDYIQGYLYLQSLYENEKNYPDAIETGKEGLRLSQFYKELMYTTGC
                     LEIEHGDANEGVLLLKQALEVDNAYQEPLLILSDLYRNEEDYEAIIELLTYVDEEDLD
                     PTFMWHLAFAYGQEERDKEAQHFFELAYPTMKTNIDFMSDYYFYLIEIGQKEQAITIL
                     NQLLELEPSNENWHDELSRLQS"
     gene            complement(3889..5187)
                     /gene="aroA"
                     /locus_tag="PPY92_001896"
     CDS             complement(3889..5187)
                     /gene="aroA"
                     /locus_tag="PPY92_001896"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDJ2625884.1"
                     /translation="MVSEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGTSNIYK
                     PLLGEDCRRTMDIFRLLGVDIKEDEDKLVVNSPGYKAFKTPHQVLYTGNSGTTTRLLA
                     GLLSGLGIESVLSGDVSIGKRPMDRVLRPLKLMDANIEGIEDNYTPLIIKPSVIKGIN
                     YQMEVASAQVKSAILFASLFSNDTTVIKELDVSRNHTETMFRHFNIPIEAERLSITTT
                     PDAIQHIKPADFHVPGDISSAAFFIVAALITPESDVTIHNVGINPTRSGIIDIVEKMG
                     GNIQLFNQTTGAEPTASIRIQYTPMLQPITIEGELVPKAIDELPVIALLCTQAVGTST
                     IKDAEELKVKETNRIDTTADMLNLLGFELQPTNDGLIIHPSEFKTNATVDSLTDHRIG
                     MMLAVASLLSSEPVKIKQFDAVNVSFPGFLPKLKLLENEG"
     gene            complement(5197..6261)
                     /locus_tag="PPY92_001897"
     CDS             complement(5197..6261)
                     /locus_tag="PPY92_001897"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDJ2625885.1"
                     /translation="MKLQTTYPSNNYPIFVEHGAIDHISTYIDQFDQSFILIDEHVNQ
                     YFADKFDDILSYENVHKVIIPAGEKTKTFEQYQETLEYILSHHVTRNTAIIAVGGGAT
                     GDFAGFVAATLLRGVHFIQVPTTILAHDSSVGGKVGINSKQGKNLIGAFYRPTAVIYD
                     LDFLKTLPFEQILSGYAEVYKHALLNGESTTQEIEQHFKDREILQSLNGMDKYIAKGI
                     ETKLDIVVADEKEQGVRKFLNLGHTFGHAVEYNHKIAHGHAVMIGIIYQFIVANILFN
                     SNHDIQHYINYLTKLGYPLETITDIDFETIYQYMLSDKKNDKQGVQMVLIKHFGDIVV
                     QHIDQTTLQHACEQLKTYFK"
     gene            complement(6287..7453)
                     /gene="aroC"
                     /locus_tag="PPY92_001898"
     CDS             complement(6287..7453)
                     /gene="aroC"
                     /locus_tag="PPY92_001898"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDJ2625886.1"
                     /translation="MRYLTSGESHGPQLTVIVEGIPANLEIKVEDINKEMFKRQGGYG
                     RGRRMQIEKDTVEIVSGVRNGYTLGSPITMVVTNDDFTHWRKIMGAAPISEEERENMK
                     RTITKPRPGHADLVGGMKYNHRDLRNVLERSSARETAARVAVGALCKVLLQQLDIDIY
                     SRVVEIGGIKDKDFYDSETFKANLDRNDVRVIDDSIAQAMRDKIDEAKNEGDSIGGVV
                     QVVVENMPVGVGSYVHYDRKLDGKIAQGVVSINAFKGVSFGEGFKAAEKPGSEIQDEI
                     LYNSEIGYYRGSNHLGGLEGGMSNGMPIIVNGVMKPIPTLYKPLNSVDINTKEDFKAT
                     IERSDSCAVPAASIVCEHVVAFEIAKALLEEFQSNHIEQLQQQIADRRQLNVEF"
BASE COUNT         2485 a         1447 c         1046 g         2755 t
ORIGIN      
        1 cgatgcaatt cattggtcta tgtttttaat cttaatcaaa tccaaataaa gcatatattt
       61 ttaaattcac tttctttcga atcgattttt atctcttgta ttaaactttt ccattgtttc
      121 attaaagctc tctgtcatat ctattcccat tgaattcgct aaacataaca acacaaataa
      181 attatcacct aattctgctt taatcgtatt tgcttcctct gaatctttct tctttttttc
      241 accataggta tgatttattt cacgtgcaag ttcgcccact tcttcagtca atctagctaa
      301 gttagctaat ggtgaaaaat atcctgtttt aaattgtcca atatattcat caacttcacg
      361 ttgcatttct accattgatt tcatttctac gttctcctta tattgcattt ctaatatagt
      421 atatatcaat ttgaagtctc atgcatgttt agtgcaatct actgactcta gctaaactag
      481 atgacaagtt gtttatttta gaagtttaat aattcagttt atataaatgt aatgcattcc
      541 taactaaatt aaatcaattg aaattgggat tataacttta tgattcgtac cactacaata
      601 aaataatata atgaataatc taccattaga aaaataagca caaaaaaact agcaatcaca
      661 caaaatgtga ttagctagtt aataagtgtc taatttaagt taattgttaa tctataagat
      721 taatcacttg aacgcgcaat caaaataata cgtacaagct ctgctacagc gacagcagtt
      781 gctgcaacat aagtcattgc tgctgcagat aatactttac gcgcatgctt gtattctttt
      841 tcatttacaa tgttcaatgc cgtaatttgt ttcatcgctc ttgaactcgc atcaaactca
      901 actggtaacg taacaattga gaataatacc gctaatgaca ttaaaccagc accaatccat
      961 aaagcagttg aaccaaatgc actacctatc gctgttaaga taatacctaa catgatgatc
     1021 atataactta atgaactccc taggtttgca acaggtacta atgctgctct gaatcttaag
     1081 aaccaatatc cttggtgatc ttgaatggca tgaccaactt cgtgggctgc aattgcagtt
     1141 ccagcaactg atggtctgtc atagtttgca ggagatagtg aaacaacttt ctttttagga
     1201 tcgtaatgat ctgttaagaa tccttcacct ttaacaactt cgacatcata aataccgttt
     1261 gcatgtaaaa tttctaatgc aacttcacga cccgttttac cactagttga tctaacttgt
     1321 gaatatttct catagttaga tttaactttg tgttgtgccc ataaaggaag caccattaat
     1381 attacgaaat aaattatcat agtaaaaatt gaagacaata aactcactct cctttataaa
     1441 tattttactg tcatttgccg tttttatcaa atcatttaca ctttaataat ttgtttaatt
     1501 caatataaag caaaagtcca aaaacactta gacaacatga taatacacca atttgccaca
     1561 catgtgtagt tataaaatca taatatggaa attgaaggtg aaaatagtca atataatcat
     1621 tcaaaaacac ccaaatcatc gctacactga ttccaatcat agaacgttta aacctaggat
     1681 agaagtaaat tgcctgaaca gccattatac tgtgggaaaa cattaatacc aaaccattta
     1741 ctgtaatatc accttgttca ataataaata atatattcat tataactgcc caaatcccat
     1801 atttgaataa tgttacaaat gccagtgctt cgataatact attttgtttt tgaattaata
     1861 tcaatgagat agaaataact aagtataata ttgcagttgg gctatctgga acaaaaatct
     1921 taaaatgcca cggcgtatga cttaattgtt caccatacca tatataacca taaatcatcc
     1981 ctaatatatt acaaatgagt agcatcatta accaagaacg ttgataaagt gtatattgcc
     2041 aaaatgtatt aatcgtcatc tgctaagtcc tcaaattgat tatgtttatt tactagcttg
     2101 agtgtattta aaatttgcgt tagttgataa aaacgttgct tttcattcat ctgtaaactt
     2161 aaatcaatat tgtgtaacaa gtaatctatt aataacgcat gtttatgccg atctatagcc
     2221 atactattta agtcatgaag ataagtttga taactgggcg attccgttag ttgagcaact
     2281 attaaatcat ctagcctttg ctcacgcttt gacacgtttg cgaagtgaat ttgaatatca
     2341 aaagcacagt tatgattagc gatataatca aatatttcat ttgtattcat taactttata
     2401 ttacgcttag taaattgaat tgcagaagcg tgacttccca cctctgcaat ttctaatgtt
     2461 tcatgatgat taatttttgt atctacaaaa tgaatgtttg ccaatttcgc ctcattcact
     2521 tttatatagt taagcaccca aactgcaata cgcgacttaa atcgatattg aaaaagtaaa
     2581 tattcaataa aactttcttt aatttgattg agtgtctctg acatcaaata ccccatttta
     2641 agattgcaat cttgataatt cgtcatgcca attttcgtta cttggctcta gttccaacaa
     2701 ttgatttaaa atagtaattg cttgttcctt ttgaccaatt tcaattaaat agaaataata
     2761 atcactcata aaatcaatat ttgttttcat cgttggatat gctaattcaa agaaatgttg
     2821 agcttcttta tctcgctctt cttgaccata ggcgaacgct aaatgccaca tgaatgtagg
     2881 atccaaatct tcttcatcta catatgttaa taattcaatg attgcttcat aatcttcttc
     2941 attacgatat aaatcgctta aaatcaataa aggttcttgg taagcattat caacctctaa
     3001 tgcttgtttt aacaataata caccttcatt agcatcgccg tgttctattt ccaaacatcc
     3061 agttgtatac attaactctt tataaaattg acttagtcgt aatccttctt tacccgtctc
     3121 aatggcatct ggataatttt tttcattttc atataatgat tgtaaataca agtagccttg
     3181 aatataatca ggatctttag aaagtaatgt agtcattatt ttaattgctt cttgagtgat
     3241 gtcattttta tcgtaagaaa tggctttttt gagataatct tctgaagtca tttcatcttc
     3301 attaatttca tcgtataagc gaatcgcatc actatagtta ccactttgta aactacaatc
     3361 tgccatacga gagaataagt ttacaccatt aacttgatat tcaccagttt ctaaaacggt
     3421 ttcgtattca gaggtagcac gtaaatattg accatcataa tataacattt cagccaatgc
     3481 aaaatggatt attggatcat ttggctctag ttcaagtgct tcttgtaatt tatcaatagc
     3541 aacttccatc atattaattt gttgatataa atctgcttct aacatcaact tttcaggtga
     3601 tggttcaaca taacttaaat attctaacgc ttcgtcagtt tgattttcag acattaaacc
     3661 ttcaataaaa taaatcagca attcactttc gtctggatat ttgtgatata acacgcggaa
     3721 tacttccaaa ccttgtggca ttaatccaaa attgtaaagt gtctctccta gaataaataa
     3781 tgcgtcatcg ttgtcagtag ttattgcttc attaacacga gagtctaaat tttctagttt
     3841 ttgtagattg atatcgtcta ttaatttata gatatcttcc attttatatt atccctcatt
     3901 ttctaaaagc tttagttttg gtaaaaatcc tggaaatgat acatttacag catcaaattg
     3961 tttgattttg acaggctcgc ttgaaagtag agaagcaact gcaagcatca ttcctattcg
     4021 atgatcagtt aaactatcaa ctgttgcatt tgttttaaat tctgacggat gaataatcaa
     4081 tccatcatta gttggttgta attcaaaccc taacaagttt aacatatcag ccgttgtatc
     4141 aattctattt gtttctttta cttttaattc ctcggcatct ttaattgtac tcgtgccaac
     4201 tgcttgtgta caaagtaatg ctattacagg cagttcatca attgcttttg gaactaattc
     4261 tccttcgatt gttattggtt gaagcattgg tgtgtattga atacgaatag aagcagtagg
     4321 ttcagcacca gttgtttgat tgaaaagttg gatattaccg cccatttttt caacaatatc
     4381 aataatacct gaacgtgttg gattgattcc aacattatga attgttacat cactttctgg
     4441 tgtgataagt gctgcaacaa taaagaacgc tgcagatgaa atatcgccag gaacatgaaa
     4501 atctgcaggt ttaatatgtt gaatggcatc aggggttgta gttattgata acctttctgc
     4561 ttcaattgga atattaaaat gtctgaacat cgtctcagta tgatttcgac ttacatctaa
     4621 ttcctttatt accgttgtat catttgaaaa caaacttgca aataaaatgg cactttttac
     4681 ttgtgcactt gcaacttcca tttgataatt tatacctttt atgacagatg gcttaataat
     4741 taatggtgta taattatctt caataccttc aatattcgca tccataagtt tcaatggtct
     4801 caagacacga tccattggcc ttttaccaat tgaaacatcg ccagacaaaa cactttcaat
     4861 acctaaacca cttaacaaac cagctaacaa tcgagtcgtt gtgccagagt ttccagtata
     4921 taaaacttga tgaggtgttt taaaagcttt atatccaggt gaattcacaa ccaatttatc
     4981 ttcatcttct ttaatatcta cgcctaataa tcggaatata tccattgtac gacgacaatc
     5041 ttcaccaagt aatggtttat aaatattgga agttccttca gctaaagatg ctaacatgat
     5101 tgctcgatgt gtcatcgact tatctccagg cacttctatt tcacctttaa gtggccctga
     5161 aatatcgata atttgttcac ttaccattta cttcacctac ttaaaatatg tttttagttg
     5221 ttcacacgca tgttgcagtg ttgtttgatc aatgtgttgc actacaatat cgccaaaatg
     5281 tttaatcaat accatttgaa ctccttgttt gtcgttcttt ttatcactta gcatgtattg
     5341 atatatcgtt tcgaaatcta tgtcggttat tgtttctaaa ggataaccta atttcgttaa
     5401 ataattaata taatgttgga tatcgtgatt agaattgaac aatatattcg caacaataaa
     5461 ttgatatatt atgcctatca ttacggcatg accgtgtgca attttgtggt tatactccac
     5521 ggcatgacca aatgtatgac ctaaatttaa aaatttacgt acaccttgtt ctttttcatc
     5581 tgcaacaaca atatccagct tcgtttcaat acctttagca atatatttat ccataccatt
     5641 taatgactgt aatatctctc tatctttaaa gtgctgttcg atttcttgcg tcgttgattc
     5701 accattcaat aacgcatgct tataaacttc tgcatagcca cttaatattt gctcaaatgg
     5761 taacgtcttt aaaaagtcta aatcataaat cacagcagtt ggacgataaa atgcaccgat
     5821 aaggttttta ccttgttttg agttaatacc cactttaccg ccaacactag aatcatgcgc
     5881 caaaatcgtt gtaggaactt gtataaaatg gacacctctt aatagtgttg ctgctacaaa
     5941 tcctgcaaaa tctcctgtcg caccaccacc aacagctata atcgctgtat tacgcgttac
     6001 atgatgtgac aaaatgtatt ctaatgtttc ttgatattgc tcaaatgttt tcgtcttttc
     6061 accagctgga ataatgactt tatgcacatt ttcatatgat aaaatatcat caaatttatc
     6121 agcaaaatat tgatttacat gctcgtcaat taatataaaa ctttgatcaa actgatcaat
     6181 atacgtgcta atatggtcaa ttgcaccatg ttcaacaaat attggataat tgtttgaagg
     6241 ataggttgtt tgtaatttca tgatcacacc tcaattgttc tcgttgttaa aactcaacat
     6301 ttaattgtct gcggtcagca atttgttgtt gaagttgttc aatatggttt gattgaaact
     6361 cttcaagtaa tgctttcgct atttcaaatg caacaacgtg ttcacacact atacttgctg
     6421 caggtacagc acaactatca gaacgttcaa ttgttgcctt aaagtcttct ttagtattta
     6481 tatcaactga atttaatggt ttatataacg taggaatagg tttcattaca ccatttacaa
     6541 taattggcat accatttgac ataccaccct ctaaaccacc taaatgatta gatccacgat
     6601 aataaccaat ttcactatta tatagaattt catcttgaat ctcactacct ggcttttcag
     6661 ctgctttaaa tccttctcca aagcttacac ctttaaaagc atttatgctg acaacacctt
     6721 gtgcaatctt accatctaac ttacgatcat aatgcacata actaccaaca ccaacaggca
     6781 tattttcaac tacaacttga acgacaccgc caattgaatc tccttcattt ttagcttcgt
     6841 caattttatc tcgcattgct tgtgcgatac tgtcatcaat tacacgaaca tcattacgat
     6901 caagatttgc tttaaatgtt tctgaatcat aaaaatcttt atctttaatg ccacctattt
     6961 caacaacacg actgtatata tcgatatcta actgttgtaa taacacttta cataaggcac
     7021 cgactgcaac tcgagctgct gtttctctag cagatgatcg ctctagcaca tttcgtaaat
     7081 cacgatgatt atatttcata cctccaacca aatctgcatg accaggtctt ggttttgtaa
     7141 tagtacgttt catattttca cgttcttctt cacttattgg agctgctccc ataatttttc
     7201 tccaatgcgt aaagtcatca ttggttacaa ccatagtaat tggactacct aatgtataac
     7261 catttctaac gcctgatact atttctactg tatctttctc aatttgcatg cgtcggccac
     7321 gaccataacc cccttgacgc ttgaacattt ctttattaat atcttcaact ttaatttcta
     7381 aatttgctgg tataccttcg acaataactg ttaattgagg tccatgtgat tctcctgaag
     7441 ttagatatct catacacact cgctcctttc atacttccgc aataacatta taatatgttg
     7501 ttaatcttat cacattcatt gtggattcat aaatgtttgt tttcaagctt aattaagaaa
     7561 atggatgaca tctcatttta aatgtcagaa ttatcttaat attaaacgaa ctaaaattaa
     7621 ttataatata ttcgtctgta gaaaagtatc atgaatttct aaacagttgc atcatataat
     7681 cattaattaa aaatgattag ccgttttaat ataatcgtat cgcaacttta gca
//
LOCUS       DANBWH010000129         7720 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.133, whole genome shotgun sequence.
ACCESSION   DANBWH010000129 DANBWH010000000
VERSION     DANBWH010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7720
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.133"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(92..418)
                     /locus_tag="PPY92_001899"
     CDS             complement(92..418)
                     /locus_tag="PPY92_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HDJ2625887.1"
                     /translation="MILDNVNPNDLFPTEKKGPSVLGIIEYQVQGENEFEGAFIATNE
                     RLIMNVDMNGQFYYRSISYNEIEKIDYDGQTIMFKFNIGNVPMHDIKSDNVEMFVEYV
                     KQHMIV"
     gene            complement(482..1225)
                     /locus_tag="PPY92_001900"
     CDS             complement(482..1225)
                     /locus_tag="PPY92_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HDJ2625888.1"
                     /translation="MSKFKVKRLTAHILMLKVINDMLKYSFHLTLTQVKLLNLLVKYD
                     NSDSNSASIDRLLKMKEIQSKMALLQMLSYLNNHQWLLKGRDQRDQRKLTISINKMHI
                     DKIEYMNNELSDYIERYFGQYTFDFSCDYVSYLLSSQELFRNLKCYLNMCQLTLEELY
                     VLGILNLHKGQLTVKELQGEFHHPIFAVSPILKCLILKGLVKKERCELDERRVIVTIK
                     REKFSKVTMLIQACYNYLEKGIQVKLNNK"
     gene            complement(1251..3206)
                     /locus_tag="PPY92_001901"
     CDS             complement(1251..3206)
                     /locus_tag="PPY92_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDJ2625889.1"
                     /translation="MTDNYSLHIYKSMKMSSVKEANLKIIYWLQGTGVVSINLQQYDV
                     KRNDVTVIMLNDLYQIDGHDDSVCCVVEVSAKTFLKFMNANYMMRGKILTNEVASTFR
                     ILIKYLIYSKIKQQSYNANDKIINYMCVELMSLNTYDGSRHLVAEEVHDYLTNNHHKK
                     INRKDVINQVSITNKALSDMFKATPYSNFIQYLNHIRLEHCLIDILTSKKPIEEIASS
                     HGFNHYSRFIHLFKETYGNTPKLIRKRYSPTSHSINHSQLVEIDKNIIELFDNTNQSS
                     SSMREIDIPFEPTKRSQMYQPKNIYIKGSNFYWMDYYTIKQIIQRLGVNLENLHIIVT
                     IDTSNELSIEQITLLLQKIVNYNVNVVFRIKHEYKELLTSTERGKIEKLVATIFNMTF
                     ESNRCKIAFLINDLNTTAIQKLKRLIDNYIGVFELIYHLEPKELNTHYDKEIDHLIDY
                     FILPLRQIEQVSIACSKIICDVDLINENNDLKEKITMSDFNKVTELLTNYTKFRGLIM
                     HMSIISEASKEINLTRLSIFIFIINILNQLRGIVIYQNDTMIVTKFKHEVQCVICLPA
                     ALLNENSDAIRLSCKGLQQFSHAKTKQIVLSIDKDNHTSENGVLVNDIMQQHSYWKAN
                     FIERNIYDHSLMVSSNSIVHLKYHLEI"
     gene            3816..4619
                     /locus_tag="PPY92_001902"
     CDS             3816..4619
                     /locus_tag="PPY92_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDJ2625890.1"
                     /translation="MKKIATATIATAGFATIAIASGNQAHASEQDNYGYNPNDPTSYS
                     YTYTIDAQGNYHYTWKGNWHPSQLNQDNGYYSYYYYNGYNNYNNYNNGYSYNNYSRYN
                     NYSNNNQSYNYNNYNSYNTNSYRTGGLGASYSTSSNNVQVTTTMAPSSNGRSISSGYT
                     SGRNLYTSGQCTYYVFDRVGGKIGSTWGNASNWANAAARAGYTVNNTPKAGAIMQTTQ
                     GAYGHVAYVESVNSNGSVRVSEMNYGYGPGVVTSRTISASQAAGYNFIH"
     gene            complement(4637..4879)
                     /locus_tag="PPY92_001903"
     CDS             complement(4637..4879)
                     /locus_tag="PPY92_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625891.1"
                     /translation="MTKLIENLKASIFVSTLTKNLSMYSRFDMPKFGCVNGCMLFILY
                     YRVNRIGENRNFEIFTNSIFFIEIKKANKALETLLA"
     gene            complement(5053..6453)
                     /gene="nhaC"
                     /locus_tag="PPY92_001904"
     CDS             complement(5053..6453)
                     /gene="nhaC"
                     /locus_tag="PPY92_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDJ2625892.1"
                     /translation="MMKRKPTFLESISTMIVMVIVVVTGFVFFDIPIQVLLIIASAYA
                     TWIAKRVGLTWQDLEKGIAERLNTAMPAILIILAVGIIVGSWMFSGTVPALIYYGLDL
                     LNPSYFLISAFFISAVTSVATGTAWGSASTAGIALISIGNQLGIPPGMAAGAIIAGAV
                     FGDKMSPLSDTTNLAALVTKVNIFKHIHSMMWTTIPASIIGLLVWFIAGFQFKGHSND
                     KQIQTLLSELAQIYQINIWVWVPLIVIIVCLLFKMATVPAMVISSFSAIIVGTFNHHF
                     KMTDGFKATFSGFNDSMIHQSHISSSVKSLLEQGGMMSMTQILVTIFCGYAFAGIVEK
                     AGCLEVLLTTISKGIHSVGSLICITVICCIALVFAAGVASIVIIMVGVLMKDLFEKYQ
                     VSRSVLSRTLEDSSTMVLPLIPWGTSGIYYTNQLHVSVGEFFMWTVPCYLCAIIAIIY
                     GFTGIGIKKSSNSRLT"
     gene            complement(6548..7630)
                     /locus_tag="PPY92_001905"
     CDS             complement(6548..7630)
                     /locus_tag="PPY92_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HDJ2625893.1"
                     /translation="MKIAIVGSGNGAVTAAVDMVSKGHDVKLYCRNQSISKFQNAIEK
                     GGFDFNNEGDERFVKFTDISDDMEYVLKDAEIVQVIIPSSYIEYYADVMAEHVTDNQL
                     IFFNMAAAMGSIRFMNVLEDRHIETKPQLAEANTLTYGTRVDFENAAVDLSLNVRRIF
                     FSTYDRSCLNDCYDKVSSIYDHLVKEESLIKTNLENGNPEVHPGPTLLNVGRIDYAGE
                     FALYKEGITKHTVRLLHAIELERLNLGRRLGFELSTAKESRIERGYLERDKEDEPLNR
                     LFNTSPVFSQIPGPNHVESRYLTEDIAYGLVLWSSLGRVIDVPTPNIDAVIVIASTIL
                     ERDFFEEGLTVEEIGLDKLDLEKYLK"
BASE COUNT         2640 a         1412 c          983 g         2685 t
ORIGIN      
        1 agaagagctt taaatcataa cgcatgccat ttatctttaa atgcaatgag ttatcattca
       61 aactcttctt atcaacctac atacattgac attatactat catatgttgt tttacatatt
      121 caacaaacat ttcaacgtta tcacttttaa tatcatgcat tgggacatta ccaatattaa
      181 atttgaacat tatagtttga ccatcataat ctatcttctc tatttcatta taactaatac
      241 ttctataata aaattggcca ttcatatcta cattcataat taatctttca tttgttgcta
      301 taaatgcccc ttcaaattca ttctcacctt gaacttgata ctcaattatt cctagcactg
      361 acggaccctt tttttcagtt ggaaataaat cattcggatt cacattatct aaaatcataa
      421 tatccctccc acttaaaact ataaactatt cttcataggt atatgaaata caagtgatta
      481 actatttatt attaagctta acttgtattc ccttttcaag ataattataa catgcttgaa
      541 taagcatagt gactttagaa aacttttctc gcttgattgt tacaataaca cgtctttcat
      601 ctaattcaca tctctctttt ttaactaatc ccttcaaaat taaacacttt aaaatcgggc
      661 taaccgcaaa tatcgggtga tggaattcac cctgtaattc tttaaccgtc aattgacctt
      721 tatgcagatt taatattcct aaaacgtata actcttctaa tgttaattga cacatattta
      781 aataacactt tagatttcga aataattctt gtgacgacaa taaatacgat acatagtcac
      841 aactaaaatc aaatgtatat tgaccaaaat aacgttcaat atagtcacta agctcattat
      901 tcatatattc tattttatct atatgcattt tattaatcga aatagtcaat ttacgttgat
      961 ccctttgatc tcttcctttc aatagccatt gatgattgtt taaataactt aacatttgaa
     1021 gaagtgccat cttagattgg atttctttca tttttaataa cctatctatt gaagcgctat
     1081 tggaatcaga attatcatat ttaactaata gatttaatag tttcacttga gtcaaagtga
     1141 gatgaaatga atatttcaac atatcattaa tgactttcaa cattagtatg tgtgcagtta
     1201 aacgcttaac tttaaattta gacatcttta aaacctctct taaaccatgc ctatatctca
     1261 agatgatatt tcaaatgaac aatactattg cttgagacca ttaatgaatg atcataaata
     1321 tttctttcta taaaattagc tttccaataa ctgtgttgtt gcataatatc attcacaagt
     1381 acaccatttt cggaagtatg attatcttta tctatactta aaacaatttg tttagtttta
     1441 gcatggctaa attgttgaag acccttacac gataaacgta tagcgtctga attctcattt
     1501 aacaatgcgg ctggcaaaca aatgacacat tgtacttcat gtttgaactt cgttacaatc
     1561 atcgtgtcat tttgataaat aacaatccct cgtaattgat taagtatatt tatgataaaa
     1621 atgaatattg ataatctagt caaatttatt tctttgctag cttcacttat aattgacatg
     1681 tgcataataa gtcctcgaaa ttttgtataa tttgtcaaaa gttcagtaac cttattaaaa
     1741 tcagacattg tgattttttc ttttaagtca ttattctcat taatcaaatc tacatcacat
     1801 ataattttag aacatgcaat agacacctgt tctatttgtc ttagcggtaa gataaaatag
     1861 tctattagat gatcaatttc tttatcatag tgcgtattca actcttttgg ttccagatga
     1921 tatattaatt caaatactcc tatataatta tctattaagc gtttcagttt ttgaattgca
     1981 gtagtattta agtcgttaat taaaaatgca atcttacatc tattactctc aaatgtcata
     2041 ttaaaaattg tggctactag cttttctatc ttgcctcttt cagtactcgt taacaattcc
     2101 ttatattcat gtttaatacg aaatactaca tttacattgt aattcactat cttttgaagt
     2161 aataaagtga tttgttcaat cgatagctca ttgcttgtgt cgattgtaac aattatatgc
     2221 aagttttcaa gattaacacc taatctttga atgatttgtt taatagtata ataatccatc
     2281 caataaaaat tacttccttt aatataaatg tttttaggtt gatacatttg actccttttt
     2341 gtaggttcaa aaggtatatc aatctcgcgc atacttgaag aactttgatt agtattatca
     2401 aataattcaa ttatattttt atcaatttca actaattggg aatggttaat tgaatgagat
     2461 gttggactat atctttttct aattaattta ggagtgttac catacgtttc tttaaagagg
     2521 tgtataaaac gagaataatg attaaaacca tgactacttg cgatttcttc aataggcttc
     2581 ttagaagtta aaatatcaat taagcaatgc tccagtctaa tatgatttaa atattgaata
     2641 aaattactat aaggtgtcgc tttaaacatg tcacttaaag ctttgtttgt aatactaact
     2701 tgattaatga catctttcct atttatcttt ttatggtggt tgtttgttaa ataatcgtgc
     2761 acttcttcgg ctactaaatg acgactacca tcgtatgtat ttaatgacat taattcaaca
     2821 cacatgtaat taataatctt atcattagca ttataagact gttgtttaat tttgctgtag
     2881 attaaatact taatcaagat tctaaaagtg ctagcaacct catttgttaa tatcttgcca
     2941 cgcatcatat agtttgcatt cataaacttc aaaaatgtct ttgcacttac ttcaacaaca
     3001 caacatacac tatcatcatg accatcaatt tgatacaaat cattcaacat aataacggtg
     3061 acatcatttc tttttacatc atattgttgt agattgattg aaactacacc tgtaccttgt
     3121 agccaatata taatttttaa gttcgcttcc ttcacactac tcattttcat acttttataa
     3181 atatgtagtg aataattatc tgtcatctta catcctcaca tatacaaata tattgattta
     3241 ctcctgtttg tctttctcaa atcaattgtc aacatctaat attcactacc aattatatcg
     3301 ccacatgttt tttagcagta acaaaacata agtcgattat atcaaataat acttttatta
     3361 aaacattttt ttatgctaaa ttaggatatt ttatccatta aatatacatt ttcatccaaa
     3421 taccaaagct ttcataatca tagaaacttg atcgttcgtg tcgaattttt atgcattttc
     3481 tgattaaaag tcctatatta tgcacaaaat atacatttag aattttcagt aatattacaa
     3541 atttgtaaca gacttatttt cgaaacattc agttttcaca aagaatgccg tagttataag
     3601 ttttgcaaaa tgcaaatttc tatactttta aaattctatt gtgtcaagat attagcaaaa
     3661 aattacaaaa cgttaacaaa gcacacaagg acgctaattt ctgttatagt ctttcttgtc
     3721 gtttaattac gagagataac ttataacatt aattttgtca cacggacatt acgaaaatat
     3781 tttagattta aaatttttag gaggatattt ttaaaatgaa gaaaatcgct acagctacta
     3841 tcgcaactgc aggattcgct acaatcgcaa ttgcatcagg aaatcaagct catgcttctg
     3901 agcaagataa ctacggttat aatccaaacg acccaacatc atatagctat acttacacta
     3961 ttgatgcaca aggtaactac cattacacat ggaaaggtaa ctggcatcca agtcaattaa
     4021 accaagataa tggctactac agctattact actacaatgg ctacaataac tacaacaatt
     4081 acaacaatgg ttatagctac aataattaca gccgttacaa caactactca aataataatc
     4141 aatcatataa ctacaataac tataatagtt acaacacaaa cagctaccgt actggtggtt
     4201 taggtgcaag ctacagcact tcaagcaaca atgttcaagt aactacaact atggctccat
     4261 catcaaatgg ccgttcaatc tcaagtggtt atacttcagg acgtaactta tacacttctg
     4321 gtcaatgtac atactacgta tttgatcgtg taggtggtaa aatcggttca acttggggca
     4381 atgcaagtaa ctgggctaac gcagctgcaa gagctggtta cacagtgaac aatacaccaa
     4441 aagctggtgc aattatgcaa acaactcaag gtgcatacgg tcacgttgca tacgttgaaa
     4501 gtgttaacag caatggttca gtaagagttt cagaaatgaa ctatggttat ggcccaggtg
     4561 ttgtaacttc acgtacaatc tcagctagcc aagctgctgg ttataacttc attcactaat
     4621 tagtgatggc tatgttttac gccaacaagg tttcaagagc cttgttggct tttttaattt
     4681 ctatgaaaaa aatcgaattg gtaaaaatct caaaatttcg attttcacca atccgattca
     4741 ctctgtaata aagaataaac aacatacagc cattgacaca cccaaattta ggcatgtcaa
     4801 atcggctata cattgataaa tttttagtta aagtacttac aaatatactt gccttcaaat
     4861 tctcaattaa tttagtcatt acaaatcatg acataaaact tacattacat atatcaacat
     4921 gttccgctac aaaacatatt cagtatatgt atagaggata tttattctaa gttaaaacct
     4981 tttcaaatta taaccgctac aattataaat gattaaagtg tttcaacata ttatatatat
     5041 tccacgctca cattaagtta aacgtgaatt cgatgacttt ttaataccta tccctgtaaa
     5101 accatagata attgctataa ttgcgcataa ataacatggt actgtccaca tgaaaaattc
     5161 tccaacagag acatgaagtt gattcgtata gtaaatacct gatgttcccc atggtattaa
     5221 aggtaagacc atcgtacttg agtcttcaag tgttcttgat aaaacggaac gagagacttg
     5281 gtatttttcg aacaaatctt tcattaacac accgaccata ataattacaa tcgaagcaac
     5341 acctgcagcg aatacaagcg caatacaaca aataacagta atacatatta aacttcctac
     5401 agaatggatg cctttagaaa tagtagttaa taagacttct aaacatcctg ctttttctac
     5461 aatacctgca aatgcatatc cgcaaaatat cgttactaat atttgggtca tactcatcat
     5521 accaccctgt tctaacaagc ttttcacact ggatgaaata tgagactgat gtatcattga
     5581 gtcgttaaaa ccactaaatg ttgctttgaa accatctgtc attttgaaat gatgattaaa
     5641 agtccccact ataatggcag aaaagcttga tattaccata gctggcactg tagccatttt
     5701 aaatagcaaa caaacaatga tcacaattaa gggaacccag acccatatgt taatttgata
     5761 aatctgtgca agctctgata acaaagtttg aatctgttta tcatttgaat gccctttaaa
     5821 ttgaaatcca gcaataaacc atactaataa acctatgatt gatgcaggta tcgtcgtcca
     5881 catcatcgaa tgtatatgtt taaatatatt aactttagta acaagtgccg ctaaattagt
     5941 tgtatctgat aatggtgaca ttttatcgcc aaacacagcc cctgctataa tagcacccgc
     6001 tgccatccct ggaggaatcc ccaattgatt accaatagaa ataagtgcaa tccctgcagt
     6061 tgatgcagag ccccatgctg taccagttgc tacagatgta acagcactta taaaaaaggc
     6121 tgatattaaa aaatagcttg gattcaataa atctaagcca taataaatca aggctggcac
     6181 tgtgccagaa aacatccaac tgcctactat aattcctacc gctagtataa ttaaaattgc
     6241 aggcattgca gtatttaaac gttctgcaat gcctttttct aaatcttgcc atgttaagcc
     6301 tacacgtttt gcaatccatg tggcatatgc tgaggcaata attaataata cttgaattgg
     6361 aatatcaaaa aacacaaagc ctgttacaac aacaataacc attacaatca ttgtcgaaat
     6421 tgattctaaa aaggttggtt ttcttttcat catctatcgt ccccttcgct tattgatgag
     6481 aaacctcttt tcttcaattt attgcttttg cataatgcta tatccttttc tatcttcaag
     6541 ccatcattta ttttaaatat ttttctaaat caagcttatc taaaccaatt tcttcaactg
     6601 ttaagccttc ctcaaagaag tctctctcta aaatggttga tgcaattaca attactgcat
     6661 ctatatttgg tgtcggtaca tcaataacac gacctaagct tgaccatagt actaaaccat
     6721 atgcaatatc ttcagttaaa tatctgcttt ctacatgatt tggtcctgga atttgtgaaa
     6781 atactgggct tgtattaaac aaacgattta atggttcatc ttctttatca cgttctaaat
     6841 aaccacgttc aatacgtgat tctttagctg ttgataattc aaaacctaat ctacggccta
     6901 aatttaaacg ttctaattcg attgcatgaa gtaatctaac tgtatgttta gtaattcctt
     6961 ctttatataa agcgaactcg ccagcataat caatgcgacc gacattcaat aatgttggtc
     7021 ctggatgcac ttcaggatta ccattttcaa gatttgtttt aattaagctt tcctctttta
     7081 ctaaatgatc ataaatactt gaaactttgt cataacaatc atttagacag cttctatcat
     7141 atgttgaaaa gaagatacga cgtacattta gagataaatc aactgctgca ttttcaaaat
     7201 cgacacgcgt accatacgtc aacgtattag cttccgctag ttgtggtttt gtttcaatat
     7261 gtctatcttc taaaacattc ataaaacgaa ttgaccccat tgctgcagcc atgttgaaga
     7321 atatcaactg attatcagtt acatgctctg ccattacatc agcataatac tctatgtatg
     7381 aagatggaat aatcacttga acaatttcag catcttttaa aacatattcc atatcatcac
     7441 taatatcagt gaattttacg aaacgttcat caccttcatt attaaaatca aatccgccct
     7501 tttcgattgc gttttgaaac ttacttatag attgattacg acaatataat ttaacatcgt
     7561 ggcctttgct caccatatct actgctgccg taactgcgcc atttcctgat cctacaattg
     7621 ctattttcat aaacaaccct ctttctatct ttggttaaaa ttaaaaacaa attatacgta
     7681 agacaatata aaaatgatgc gaattcatta cgcatcatta
//
LOCUS       DANBWH010000130         7681 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.134, whole genome shotgun sequence.
ACCESSION   DANBWH010000130 DANBWH010000000
VERSION     DANBWH010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7681
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.134"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..259
                     /locus_tag="PPY92_001906"
     CDS             <1..259
                     /locus_tag="PPY92_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625894.1"
                     /translation="NKLLDKLHKALKDREEYKKQRDELIGDIAKLRERNKDLEKKASA
                     WDRYCKSVEKDLINEFGNDDERVKFGMKLNNKIFMEDDTNE"
     gene            252..500
                     /locus_tag="PPY92_001907"
     CDS             252..500
                     /locus_tag="PPY92_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HDJ2625895.1"
                     /translation="MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEILE
                     GMTNAIQHSVKEGIELDEAVGIMTSQVVYKYEEAQEND"
     gene            493..1023
                     /locus_tag="PPY92_001908"
     CDS             493..1023
                     /locus_tag="PPY92_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP pyrophosphatase"
                     /protein_id="HDJ2625896.1"
                     /translation="MTNTLQVKLLSKDARMPERNHKTDAGYDIFSAETVVLEPQEKAV
                     IKTDVAVSIPEGYVGLLTSRSGVSSKTYLVIETGKIDAGYHGNLGINIKNDMEHDGIT
                     SLYEDLDDKLVNTLDIKGNYINEGEGARKVYKINKGDKLAQLVIVPIWTPELKQVEEF
                     ESVSERGAKGFGSSGV"
     gene            1060..1266
                     /locus_tag="PPY92_001909"
     CDS             1060..1266
                     /locus_tag="PPY92_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HDJ2625897.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAKEKY
                     EKQVKRDAVIKVGQLFENIRECGK"
     gene            1263..1649
                     /locus_tag="PPY92_001910"
     CDS             1263..1649
                     /locus_tag="PPY92_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625898.1"
                     /translation="MIKKLKNMDGFDIFIVGILSLFGITALLLVVALPIYTVASYQHK
                     EVHQGTITDKYNKRQDKEDKFYIVLDNKQVIENSDLLFKKKFASADIQARLKVGDKVK
                     VKTIGYRIHFLNLYPVLYEVKKVDKQ"
     gene            1646..1798
                     /locus_tag="PPY92_001911"
     CDS             1646..1798
                     /locus_tag="PPY92_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDJ2625899.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDYEKI
                     RAEVSW"
     gene            1866..2066
                     /locus_tag="PPY92_001912"
     CDS             1866..2066
                     /locus_tag="PPY92_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HDJ2625900.1"
                     /translation="MWIVISIVLAIFLLILLSSISHKMKTIEALEYMNSYLFKQLVKN
                     NGVEGLEDYENEVERIRKRFKS"
     gene            2354..4801
                     /locus_tag="PPY92_001913"
     CDS             2354..4801
                     /locus_tag="PPY92_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated E family protein"
                     /protein_id="HDJ2625901.1"
                     /translation="MLDKVTQIETIKYDRDVSYSYAASRLSTYWTNHNMAWSDFMQKL
                     AQTVRTKEDLTEYNKMSKSEQADIKDVGGFVGGYLKEGKRRAGQVMNRSMLTLDIDYA
                     AQDMTDILSMFYDFAYCLYSTHKHREISPRLRLVIPLKRNVNADEYEAIGRKVADIVG
                     MDYFDDTTYQPHRLMYWPSTSNDAEFFFTYEDLPLLDPDKILNEYVDWTDTLEWPTSS
                     KEESKTKRLADKQGDPEEKPGIVGAFCRAYTIEEAISTFIPDLYEKHSTNRYTYHEGS
                     TAGGLVLYENNKFAYSHHNTDPVSGMLVNSFDLVRIHLYGAQDEDAKTDTPVNRLPSY
                     KAMQQRAQNDEVVKKQLINDKMSDAMQDFDEIVNSDDAWSETLEITSKGTFKASIPNI
                     EIILRNDPNLKGKIAFNEFTKQIECLGKVPWNTNFKTRQWQDGDDSSLRSYIEKIYDI
                     HHSGKTKDAIISVAMQNAYHPVRDYLNKISWDGHKRLEKLFIKYLGVEDTEVNRTTTK
                     KALTAGIARVMEPGCKFDYMLTLYGPQGVGKSALLKKLGGAWFSDSLVSVTGKEAYEA
                     LQGVWLMEMAELAATRKAEVEAIKHFISKQVDRFRVAYGHYIEDFPRQCIFIGTTNKV
                     DFLRDETGGRRFWPMTVNPERVEVNWSKLTKEEIDQIWAEAKYYYEQGEELFLNPELE
                     EEMRSIQSKHTEESPYTGIIDEYLNTPIPSNWDDLTIFERRRFYQGDVDMLPTGNVDY
                     VERNKVCALEVFVECFGKDKGDSRGSMEIRKISNILRQLDNWSVYDGNKSGKIRFGKD
                     YGVQIAYVRDESLEDLI"
     gene            complement(4904..5008)
                     /locus_tag="PPY92_001914"
     CDS             complement(4904..5008)
                     /locus_tag="PPY92_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625902.1"
                     /translation="MNLMQHWKQTGLNPTARRERGVVSFVASMLHHQK"
     gene            5142..5432
                     /locus_tag="PPY92_001915"
     CDS             5142..5432
                     /locus_tag="PPY92_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HDJ2625903.1"
                     /translation="MKESTLEKYLVKEITKLNGLCLKWVAPGTRGVPDRIIIMPEGKT
                     YFVEMKQEKGKLHPLQKYVHRQFENRDHKVYVLWNKEQVNTFIRMVGGTFGD"
     gene            5413..6780
                     /locus_tag="PPY92_001916"
     CDS             5413..6780
                     /locus_tag="PPY92_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDJ2625904.1"
                     /translation="MEHLAIDFKPHSYQKYAIDKVIDNEKYGLFLDMGLGKTVSTLTA
                     FSELQLLDTKKMLVIAPKQVAKDTWVDEVDKWNHLNHLKVSLILGTPKERNDALNTEA
                     DIYVTNKENTKWLCDQYKKEWPFDMVVIDELSTFKSPKSQRFKSIKKKLPLINRFIGL
                     TGTPSPNSLQDLWAQVYLIDRGERLESSFSRYRERYFKPTHQVSEHIFKWELRDGSEE
                     KIYKQIEDICLSMKAKDYLDMPDRVDTKQTVVLSEKERKVYEELEKNYILESEEEGTV
                     VAQNGASLSQKLLQLSNGAVYTDEEDVRLIHDKKLDKLEEIIEESQGQPILLFYNFKH
                     DKERILQRFKEATTLEDSNYKERWNSGDIKLLIAHPASAGHGLNLQQGGHIIVWFGLT
                     WSLELYQQANARLYRQGQNHTTIIHHIMTDNTIDQRVYKALQNKELTQEELMKAIKAR
                     IAKHK"
     gene            6793..7230
                     /locus_tag="PPY92_001917"
     CDS             6793..7230
                     /locus_tag="PPY92_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDJ2625905.1"
                     /translation="MGKASYDIKPGTFKYIESEIYNLNENKKEINRLRMEILNPTKEL
                     DTNIVYGPLQKGEPVRTTELMATRLLTNKMLRNLEEMVEAVESEYLKLPEDHKKVIRL
                     KYWNKDKKLKIEQIGDACHMHRNTVTTIRKNFVKAIAYHAGIK"
     gene            7387..>7681
                     /locus_tag="PPY92_001918"
     CDS             7387..>7681
                     /locus_tag="PPY92_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDJ2625906.1"
                     /translation="MTKHNNIYKHGRKSYQYDWFYHSKAWKKLREIALDRDNYLCQMC
                     LREDIITDAKIVHHIIYVDEDFNKALDLDNLMSVCYSCHNKIHANDNDKSNLKK"
BASE COUNT         2932 a         1004 c         1585 g         2160 t
ORIGIN      
        1 taacaagtta ttagataaac ttcacaaagc attaaaagat cgtgaagagt acaagaagca
       61 acgtgatgag cttattggag atatagctaa gttaagagag cgcaacaaag atctggagaa
      121 gaaagcgagc gcatgggata ggtattgcaa gagtgttgaa aaagatttaa taaacgaatt
      181 cggcaacgat gatgaaagag ttaaatttgg aatgaaatta aacaataaaa tttttatgga
      241 ggatgacact aatgaataac cgcgaacaaa tcgaacaatc agttatcagc gctagtgcgt
      301 ataacggtaa tgacacagag ggattgctaa aagagattga ggacgtgtat aagaaagcac
      361 aagcgtttga tgaaatactt gagggaatga caaatgctat tcaacattca gttaaagaag
      421 gtattgaact tgatgaagca gtagggatta tgacgagtca agtggtctat aaatatgagg
      481 aggcgcagga aaatgactaa cacattacaa gtgaaactat tatcaaaaga cgctagaatg
      541 cccgaacgaa atcataagac ggatgcaggt tatgacatat tctcagctga aaccgtcgta
      601 ctcgagccac aagaaaaggc agtgattaaa acagatgtag ctgtgagtat accagagggc
      661 tatgtcggac tattaactag tcgtagtggt gtaagtagta aaacgtattt agtgattgaa
      721 acaggcaaga tagacgcggg atatcatggc aatttaggga ttaatatcaa gaatgatatg
      781 gagcatgacg gcataacatc attatacgaa gatttagacg acaaactagt aaatacttta
      841 gatataaaag gtaattatat aaacgaagga gaaggcgcta gaaaggtata taaaatcaac
      901 aaaggcgaca aactagctca attggttatt gtgcctatat ggacaccgga actaaagcaa
      961 gtggaggaat tcgagagtgt ttcagaacgt ggagcaaaag gcttcggaag tagcggagtt
     1021 taaagacatc ttagatcgag tcaaggaggt tttggggaag tgacacaata cttagtcaca
     1081 acattcaaag attcaacagg acgtaaacat acacacataa ctaaagctaa gagtaatcaa
     1141 aggtttacag ttgttgaggc agagagtaaa gaagaagcga aagagaagta cgagaaacaa
     1201 gttaaaaggg atgcagttat taaagtgggt cagttgtttg aaaatataag ggagtgtggg
     1261 aaatgattaa aaaacttaaa aatatggatg ggttcgacat ctttattgtt ggaatactgt
     1321 cattattcgg tataaccgca ttgctacttg ttgtcgcatt gcctatctat acagtggcta
     1381 gttaccaaca caaagaagta catcaaggga caattacaga taaatataac aagagacaag
     1441 ataaagaaga taagttctat attgtattag acaacaaaca agtcattgaa aactctgact
     1501 tattattcaa aaagaaattt gctagcgcag acatacaagc taggttaaaa gtaggtgata
     1561 aagtaaaagt taagacgatt ggatatagaa tacacttttt aaatttatat ccggtcttat
     1621 acgaagtaaa gaaggtagat aaacaatgat taaacaaata ctaagactat tattcttact
     1681 agcaatgtat gagttaggta agtatgtaac tgagcaagtg tatattatga tgacggctaa
     1741 tgatgatgta gaggcgccga gtgattacga aaaaatcaga gctgaagttt catggtaata
     1801 gctattatca tttttgaatt aattatatta atgtgtttag caatagcact ggaggtgttg
     1861 taaatatgtg gattgtcatt tcaatcgttt tagctatatt tttattgatc ttgttaagta
     1921 gcatttctca taagatgaaa accatagaag cattggagta tatgaattct tatcttttca
     1981 agcagttagt aaaaaataat ggtgttgaag gtttagaaga ttatgaaaat gaagttgaac
     2041 gaattagaaa aagattcaaa agctaaagag ggggctaaag ccctctccag gattaaaaga
     2101 agcggatatt ttttatttcg tttttgtaaa ttaaaactaa tttatgtgaa attgtctttg
     2161 aagtcttgat aattaagaag gttgaatttt ctgagattat attagttacg ggaaaggctt
     2221 ttcctgagtg caataaaatt aaagttctca agttttcatt ttcgtattta tcataaatga
     2281 tttcgcgtaa agttttcaaa atttgtcacc tctttaattt tattaaatta attatacaag
     2341 aaaggagccg aatatgttag acaaagtcac tcaaatagaa acaattaaat atgatcgtga
     2401 tgtctcatat tcttatgctg ctagtcgttt atccacatat tggactaatc acaatatggc
     2461 ttggtctgac tttatgcaga agctagcaca aacagttaga actaaagaag atttaactga
     2521 gtacaataaa atgtctaagt ctgaacaagc agatataaaa gatgttggcg gatttgtcgg
     2581 tggatattta aaagaaggca aacgacgtgc tggtcaagtc atgaatcgtt caatgctaac
     2641 acttgatatc gattatgctg ctcaagatat gactgacata ttatctatgt tttatgattt
     2701 tgcatattgt ttatattcaa cacataagca tagagagata agtccaagac tgcgtttagt
     2761 gattccttta aaacgaaatg taaatgcaga tgagtatgaa gctattgggc gtaaagtcgc
     2821 agatatcgtt ggcatggatt acttcgatga tacaacttat caaccacata ggttaatgta
     2881 ttggccttca actagcaatg atgcagaatt tttctttacc tatgaagatt tacctttgtt
     2941 agatccagat aaaatattaa atgaatatgt tgattggact gacacattag aatggccaac
     3001 gtcttcaaag gaagagagta agactaaaag attagcagat aagcaaggtg acccagaaga
     3061 aaagccggga attgttggcg cattttgtag agcctatacg atagaagaag ctatatcaac
     3121 ttttattcct gacttatacg aaaaacattc tactaaccgt tatacctatc atgaaggttc
     3181 aactgcaggt ggattggtgt tatacgaaaa taacaagttt gcctattctc atcataatac
     3241 ggatcccgtt agcggtatgc ttgtgaacag ttttgattta gtacgcatac acttatatgg
     3301 tgctcaagat gaagacgcta aaacagatac tccggttaat cgactaccta gttataaagc
     3361 aatgcagcaa agagcgcaaa atgatgaagt tgttaaaaag caattaatta acgacaaaat
     3421 gtctgatgca atgcaggatt tcgatgaaat agtaaatagc gatgatgcat ggtctgagac
     3481 gttagaaatt acttcgaaag gtactttcaa agctagtatc ccaaatatag aaattatatt
     3541 gcgtaatgat ccaaatttaa aaggaaaaat agcatttaat gaatttacaa aacaaattga
     3601 atgcttaggg aaagtgccat ggaatactaa ttttaagaca cgtcaatggc aagacggtga
     3661 tgatagcagt ttaagaagtt atatcgaaaa gatttatgac atacaccatt caggtaaaac
     3721 aaaagatgcc attataagcg tagcaatgca aaatgcttat catccagtaa gggattatct
     3781 aaataaaata tcgtgggatg gacataaacg tcttgaaaag ttatttatca aatacttagg
     3841 tgttgaagat actgaagtga atagaacaac taccaaaaaa gcattgactg ctggaattgc
     3901 tcgagtaatg gagcctggat gtaaatttga ctatatgctt acactttatg gtcctcaagg
     3961 tgtaggtaaa tctgctttgc taaaaaaatt aggtggtgca tggttttctg acagtttagt
     4021 ttctgttaca ggtaaagaag cctatgaggc cttacaaggc gtttggctaa tggaaatggc
     4081 agaacttgca gctacaagaa aagctgaagt tgaagctatt aagcatttca tatctaaaca
     4141 agttgaccgg tttcgtgttg cttatggaca ttatattgaa gattttccaa ggcaatgtat
     4201 tttcattggt acaactaata aagttgattt cttaagagat gaaactggtg gaagacgttt
     4261 ttggccaatg actgtaaatc cagagagagt tgaagtgaac tggtctaaac taaccaaaga
     4321 agagatcgac caaatttggg cagaagctaa atattattat gaacaaggag aagagttatt
     4381 cctcaaccct gaactagaag aagaaatgcg ttcaatacaa agcaaacata ctgaggaatc
     4441 tccatataca ggcattattg atgaatatct taacacacca attcctagca attgggatga
     4501 cttaactatc tttgaacgaa gacgatttta tcaaggtgat gttgatatgt taccaacagg
     4561 aaatgtagat tacgttgaaa gaaataaggt ctgtgcgctt gaagtgtttg ttgaatgttt
     4621 tggtaaagat aagggagata gtagaggatc tatggaaatt agaaagattt caaacatctt
     4681 aagacaatta gacaattggt ctgtatatga tggtaataaa agtgggaaaa ttcgattcgg
     4741 aaaagattat ggtgtacaga tagcgtatgt aagagatgaa agtttagagg atttaatata
     4801 agaaatattg aataaatata catttttaga tgttgtatca aatgttgcat cattttttga
     4861 gtgatgcaac acggtggtgt aaaaagtaat cttaggtgtt gtatcatttt tggtgatgca
     4921 acattgatgc aacaaatgat acaacacctc tttcccttct cgctgtaggg ttcaaccctg
     4981 tttgtttcca atgttgcatc aaattcacta taaagtttaa aaagtagtgt tagggagtaa
     5041 aggggtatag gggtaaccct ctaacagcta tttttaaaag tttggcaaga attgatgcaa
     5101 catcggaaca caaatataaa ttttgtatac aaggtgaata tatgaaagaa tcgacattag
     5161 aaaaatattt agtgaaagag ataacaaagc taaacggttt atgtttaaaa tgggttgcac
     5221 ctggaacaag aggtgtgcca gatagaatta ttattatgcc agaaggaaaa acatattttg
     5281 tagaaatgaa gcaagaaaaa ggaaagttgc atcctttaca aaaatatgtg catagacaat
     5341 ttgaaaatag agatcataaa gtatatgtgt tatggaataa agaacaagta aatactttta
     5401 tcagaatggt aggtggaaca tttggcgatt gacttcaaac cacatagcta tcaaaagtat
     5461 gcaatagata aagtgataga taatgagaaa tacggtctgt ttttagatat gggtctaggg
     5521 aaaacagtat caacacttac agcatttagt gaattgcagt tgttagacac taaaaaaatg
     5581 ttagttatag cacctaaaca agttgctaaa gatacatggg ttgatgaagt tgataagtgg
     5641 aaccatttaa atcatctgaa agtgtcttta atcttaggaa cacctaaaga aagaaatgat
     5701 gcattaaaca cagaggctga tatctatgta accaataaag aaaatactaa atggttatgt
     5761 gatcaatata aaaaagaatg gccatttgac atggttgtga ttgatgaact gtctacattt
     5821 aaaagtccta agagtcaaag gtttaaatct attaaaaaga aattaccact cattaataga
     5881 tttataggat taacaggaac acctagtcca aatagtttac aggatttatg ggctcaagtt
     5941 tatttgatag acagaggtga aagacttgag tcttcattca gtcgttatcg agaaaggtac
     6001 tttaaaccaa ctcatcaagt tagcgaacat atttttaagt gggagctaag agacggatct
     6061 gaagaaaaga tatataaaca aatagaagat atatgtttaa gcatgaaagc gaaagattat
     6121 ctggatatgc ctgacagagt tgatactaaa caaacagtag tcttatcaga aaaagaaaga
     6181 aaagtatatg aagaattaga aaaaaactat attttagaat cggaagaaga aggaacagtt
     6241 gtagctcaaa atggggcatc attaagtcag aaactacttc aactatctaa cggcgcagtt
     6301 tatacagatg aggaagatgt aagacttata catgataaga agttagataa gttagaggaa
     6361 attatagagg agtctcaagg ccaaccaata ctattgtttt ataacttcaa acacgataaa
     6421 gaaagaatac ttcaaaggtt taaggaagca accacattag aggattcaaa ctataaagaa
     6481 cgttggaaca gtggagacat taagttgctt atagcacatc cagcaagtgc aggacatgga
     6541 ttaaacttac aacaaggtgg gcacattatt gtttggtttg gacttacatg gtccttggaa
     6601 ttataccaac aagcaaatgc tagattatat agacaaggac aaaatcatac gactattatt
     6661 catcacatca tgaccgataa cacaatagat caaagagtat ataaagcttt acaaaataaa
     6721 gaactaacgc aagaagaatt gatgaaagct attaaagcaa gaatagctaa gcataagtaa
     6781 tggaggtata agatgggaaa ggcatcatat gatattaagc caggaacatt taaatatatt
     6841 gaatcagaaa tatataattt aaatgagaac aagaaagaga taaatagatt gagaatggag
     6901 atacttaacc caacgaaaga actagacacc aacattgtgt atggaccgtt acaaaaagga
     6961 gagccagtta gaacaactga gttaatggcg acaaggttat tgactaataa gatgttacgt
     7021 aacttagaag agatggttga agcagttgaa agtgagtact taaagttacc tgaagatcat
     7081 aagaaagtaa taaggttaaa gtattggaat aaagataaga agctaaagat agaacaaata
     7141 ggggatgctt gtcacatgca tcgcaataca gttactacaa tacgaaagaa ctttgttaaa
     7201 gcgatagcgt atcatgcagg tatcaaataa cattgtgcaa agattgtgca aaaggcctac
     7261 aaatctgtag taatatgata gtatcggaaa gatgtataaa gttatctgaa agttatacga
     7321 cataaataca tgaggcgcat cgctaagcgg tgtgtctttt gttatgcaat caaagaggtg
     7381 taagagatga ccaagcataa taacatttat aagcatggtc gtaagtcata tcaatacgat
     7441 tggttctatc attcaaaagc atggaagaag ttaagagaga tagcattaga tagagataat
     7501 tatctttgtc aaatgtgttt acgcgaagat attataacag atgcaaagat tgtgcatcac
     7561 attatttatg ttgatgaaga ttttaacaaa gctttagact tagataatct aatgtcagtt
     7621 tgttatagct gtcataacaa aattcatgca aatgataatg acaaaagtaa tcttaagaaa
     7681 a
//
LOCUS       DANBWH010000131         7656 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.135, whole genome shotgun sequence.
ACCESSION   DANBWH010000131 DANBWH010000000
VERSION     DANBWH010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7656
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.135"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            302..3256
                     /gene="fdhF"
                     /locus_tag="PPY92_001919"
     CDS             302..3256
                     /gene="fdhF"
                     /locus_tag="PPY92_001919"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HDJ2625907.1"
                     /translation="MQEHLVVTLDGKDYLVEPGTNLLEFIKSQDTFVPSICYNESMGP
                     IQTCDTCTVEIDGKIERSCSTVIDRPMTVNTVNNDVKDAQKEALDRILEKHMLYCTVC
                     DYNNGDCEIHNTMDAWGLQHQTYEYKEKPYEKDYGPFYRYDPNQCILCGRCVEACQDI
                     EVNETIRIDWDREHPRVIWDNDVPINESSCVSCGQCATVCPCNAMMEVNMEGNAGYMT
                     DTEPGSLAAMIDLTKKAEPGYGPLFAISDSEAEMRKERIKKTKTVCTYCGVGCSFEVW
                     TKDREILKVQPSHDSPANKIATCVKGKFSWGHINSDQRLTKPLVRKNGEFHEVEWDEA
                     LNVIADNFTSIKEKYGPDALSFISSSKATNEESYLMQKLARQVIGTNNVDNCSRYCQA
                     PATKGLFRTVGHGGDSGSIEDLEKAAMSVLIGTNTAEAHPVIASRMKRAQKLFGQKIH
                     VFDIRKHEMAERADRFYQPKPGTDLAWLSAVTKYIIDHDLHDKAFIDEWVDDFDEYYK
                     SLETFTMAFAEEATGIPESELIKFAEECAKAESVVICWAMGITQQDIGSDSSTAISNL
                     LLVTGNYRRPGTGAYPLRGHNNVQGCSDMGSMPDKITGYQSIEADDIRAKFEKEYGVK
                     LNPKAGKDNHEMVEGIHDGEVHSLYLYGEDTGIVDSNINFVQAAFEKLDFMVVQDEFL
                     TFTATYADVVLPASPSLEKDGTFTNTERRIQRLYQALEPLGDSKPDWKIFQAIANRLG
                     FDWNYKHPSEIMDEVARLTPLYAGVSYDRLEGFNSLQWPVQPDGTDEPILYLEGFNFD
                     NGKAKLFPLSFDNYFKQDEIYDIHVNNGRLLEHFHEGNMTYQTPMIKYKVPRAFVEIS
                     PELAEDRGIHEGAEVKLISETGEAVLQVHVTDRVKGKEIYIPLNNDAMENGDLGAINL
                     LTNSDVDQYTDTPSYKRTSCRLEVITKRGKSPLNPNNFRVNKKRHPQYSVQVQKKWER
                     SDYVFPGNQVDK"
     gene            3256..3729
                     /locus_tag="PPY92_001920"
     CDS             3256..3729
                     /locus_tag="PPY92_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1641 domain-containing protein"
                     /protein_id="HDJ2625908.1"
                     /translation="MAERISSKIRRLEKSEEQIKLESLNEVTEAIAANKDSILKAIKL
                     IKTLDDAKLLDALNGAIRGRQVIINKFAVELNKDIYTGLLSNMASMVFLLGELNVSDL
                     SDFLNKVNKGLHVANQASPNAKTTIRSLLGVLKDDDMNRSLTYMLNMLKGMSREE"
     gene            3957..4190
                     /locus_tag="PPY92_001921"
     CDS             3957..4190
                     /locus_tag="PPY92_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625909.1"
                     /translation="MLKTSAVISNTLLVIGLVCLFMMKLVLAITFFAVSLSISLVVFN
                     MMFRDRTTMKVIVNASFLIVILAIVVAYFLLSK"
     gene            4212..4988
                     /locus_tag="PPY92_001922"
     CDS             4212..4988
                     /locus_tag="PPY92_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HDJ2625910.1"
                     /translation="MKKNFKLRISTLLLIVILVVFAVLLIVNETKLFKNDVNYSFDEA
                     VSMQQGKGIVQTKEEDGKFVEANNNEIAKAMTISHKDNDMKYMDITEKVPMSESEVNQ
                     LLKGKGILENRGKVFLEAQEKYEVNVIYLVSHALVETGNGKSELAKGIKDGKKRYYNF
                     FGIGAFDSSAVRSGKSYAEKEQWTSPDKAIIGGAKFIRNEYFENNQLNLYQMRWNPEN
                     PAQHQYASDIRWADKIAKLMDKSYKQFGIKKDDIRQTYYK"
     gene            5299..6423
                     /locus_tag="PPY92_001923"
     CDS             5299..6423
                     /locus_tag="PPY92_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HDJ2625911.1"
                     /translation="MKIAIIGAGIGGLTAAALLQEQGHTIKVFEKNESVKEIGAGIGI
                     GDNVLKKLGNHDLAKGIKNAGQILSTMTVLDDKDRLLTTVKLKSNTLNVTLPRQTLID
                     IIKSYVKDDVIFTNHEVTHIDNETDKVTIHFAEQESEAFDLCIGADGIHSKVRQSVNA
                     DSKVLYQGYTCFRGLIDDIDLKHPDCAKEYWGRKGRVGIVPLLNNQAYWFITINSKEN
                     NHKYSSFGKPHLQAYFNHYPNEVREILDKQSETGILLHNIYDLKPLKSFVYGRTILLG
                     DAAHATTPNMGQGAGQAMEDAIVLVNCFNAYDFEKALQRYDKIRVKHTAKVIKRSRKI
                     GKIAQYRSRLFVAVRNRIMKMMPNALAAGQTKFLYKSKEK"
     gene            complement(6515..7468)
                     /locus_tag="PPY92_001924"
     CDS             complement(6515..7468)
                     /locus_tag="PPY92_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HDJ2625912.1"
                     /translation="MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKN
                     ATALISLLSTNVDKDVIDAGKDLKIIANYGAGFNNIDIEYAREKSIDVTNTPKASTNA
                     TADLTIGLVLAVARRIVEGDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAV
                     ARRARAFDMDVLYTGPNRKEEKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLID
                     TEQFKMMKSTVYLINASRGPIVHEQALVQALKDNEIEGAALDVYEFEPDITDDLKSLN
                     NVVLTPHIGNATFEARDMMSKIVANAAISAVQGEKPQFVVN"
BASE COUNT         2720 a         1078 c         1473 g         2385 t
ORIGIN      
        1 tattgtcatt aataaggttg tgaaagatat gactgaaaag cgtgaaacag ataattggaa
       61 aactaataat gataattatt ttcaattaaa caaaaagatg atttaatttc gcgcaaaata
      121 tttcaaataa aaatgattgt aaaaaggcaa aatacaaaat ttcacttaac aactagtaca
      181 taaagtaata caattaaatt aattctatct gaaagatgtg tggggcatcg ttattttagg
      241 tggatatgag caatttatta aaagtcattt acggaaaata tatatagacg gggtgagtaa
      301 tatgcaagaa catttggtgg ttacacttga tggaaaagat tatcttgtag aaccaggtac
      361 gaatttactt gaatttatta aatcacaaga tacttttgta ccttcaattt gttataacga
      421 gtcgatggga ccaattcaaa catgtgatac atgtactgtt gagattgacg gtaaaattga
      481 acgttcatgt agtacggtga ttgatcgtcc aatgactgta aatactgtga acaatgatgt
      541 gaaagatgct caaaaagaag cgcttgatcg aattttagaa aagcatatgc tgtattgtac
      601 agtatgtgat tataataatg gtgattgtga aattcataat acgatggatg catgggggct
      661 tcagcatcaa acgtatgaat ataaagagaa gccatatgaa aaggattatg gcccatttta
      721 tcgttatgat ccaaatcaat gtatcttatg tgggcgttgt gtagaagcat gtcaagatat
      781 cgaagtgaat gaaactatta gaattgattg ggatcgtgaa catccacgtg ttatttggga
      841 taatgatgta ccaattaatg aatcgtcatg tgtatcttgt ggtcaatgcg caactgtatg
      901 tccatgtaat gccatgatgg aagtgaatat ggaaggtaat gcgggttata tgactgatac
      961 ggaacctggt tcattagcag caatgattga tttaactaaa aaagcagaac ctggttatgg
     1021 tccactattt gcaatctcag attcagaagc agaaatgcgt aaagagcgta ttaagaaaac
     1081 taaaacagtg tgtacttatt gtggtgtggg ttgttcattt gaagtttgga ctaaagatag
     1141 agaaatttta aaagtacaac catcacatga ttctccagca aataaaattg cgacttgtgt
     1201 taaaggtaag ttttcatggg gacatattaa ttcagatcaa cgcttaacta aaccactagt
     1261 aaggaaaaat ggtgagttcc atgaagtaga atgggatgaa gctttaaatg tcattgcaga
     1321 taattttaca tctattaaag aaaagtatgg cccagatgca ctatcattca tttcttcttc
     1381 taaagcgacg aatgaagaat cgtatttaat gcaaaaatta gcaagacaag ttattggcac
     1441 aaataacgtt gataactgtt caagatattg ccaagcacct gcaacaaaag gcttatttag
     1501 aacggttgga cacggcggtg actcaggtag tattgaagat ttagaaaaag cggcaatgtc
     1561 tgtattgata ggtaccaata cagccgaagc tcatccagtt atcgcatcac gcatgaaacg
     1621 tgctcaaaaa ttatttggac aaaaaataca tgtatttgat attagaaaac atgaaatggc
     1681 agaacgtgcg gatcgttttt atcaacctaa accaggtacg gatttagcgt ggttaagtgc
     1741 agtgactaag tatattattg atcatgattt acacgataaa gcatttattg atgagtgggt
     1801 agatgatttt gatgaatatt acaaatcatt agaaacattt acaatggctt ttgctgaaga
     1861 agcaacaggt attcctgaat cagaattgat taaatttgct gaagaatgtg ctaaagctga
     1921 atctgttgta atttgttggg caatgggtat tacacaacaa gacattggta gtgactcaag
     1981 tacagcgatt tcaaacttat tattagtaac aggtaattat cgtcgtcctg gtactggtgc
     2041 gtatccatta cgtggacata ataatgttca aggatgtagt gatatgggaa gtatgcctga
     2101 taagattact ggttatcaaa gtattgaagc ggatgatatt cgcgctaaat ttgaaaaaga
     2161 atatggcgtt aaattgaatc caaaagctgg taaagataat catgaaatgg tagaaggtat
     2221 acatgacgga gaagtacact cattgtactt atatggtgaa gatacgggta ttgtggattc
     2281 aaatattaat tttgtacaag ctgcgtttga aaaattagat ttcatggtag tccaagatga
     2341 atttttaaca ttcacagcaa catacgcaga tgttgtattg ccagcaagtc cttcacttga
     2401 aaaagacggt acttttacaa ataccgaacg tcgtattcaa cgtttatacc aagcattaga
     2461 acctcttggt gattcaaaac ctgactggaa aattttccaa gcaattgcta atagattagg
     2521 atttgattgg aattacaagc atcctagtga aattatggat gaagtagcac gcttaacacc
     2581 tctatatgct ggggtaagtt atgatcgttt agaaggattc aatagtttac aatggccagt
     2641 acaacctgat ggtactgatg agcctattct atacttagaa ggattcaatt ttgataatgg
     2701 taaagcaaaa ttattcccat tatcatttga taattacttt aagcaagatg aaatttatga
     2761 tattcatgta aacaatggtc gattgttaga gcacttccat gaaggtaata tgacttatca
     2821 aacaccaatg attaaataca aagtgccacg tgcatttgtt gaaatttctc cagagcttgc
     2881 tgaagataga ggcattcatg aaggtgcaga agttaagctg atttctgaaa caggagaagc
     2941 ggtattacaa gttcacgtga cagatcgtgt taaaggtaaa gaaatctata ttccattaaa
     3001 taatgatgcg atggaaaatg gagatctagg tgcaattaac ttattaacta atagtgatgt
     3061 tgatcaatac acggatacac catcttataa acgtactagc tgtcgtttgg aggtaattac
     3121 gaaacgtggt aagtcaccgt tgaaccctaa taatttccgt gtcaataaaa aacgtcatcc
     3181 gcagtacagt gttcaagtac agaaaaaatg ggaacgttca gattatgttt tcccgggaaa
     3241 tcaggtggat aaataatggc tgagagaata tcttctaaaa ttagacgctt agaaaaatca
     3301 gaagaacaaa ttaagttaga aagtttaaat gaagttacag aggcaattgc agcgaataaa
     3361 gatagtatat taaaagcaat caagcttatt aaaacattag atgatgcaaa actattagat
     3421 gctttaaatg gtgcaattcg aggacgtcaa gtcatcataa ataaatttgc agttgaactt
     3481 aataaagata tttatacagg gctattatct aatatggctt caatggtatt tttattaggt
     3541 gaactcaatg tatcagattt aagcgacttc ctaaataaag ttaataaagg attacacgtt
     3601 gccaatcaag cgagtccaaa tgctaagaca actataagaa gtctattggg cgtattgaaa
     3661 gatgacgaca tgaaccgtag tttaacatac atgcttaata tgctaaaagg tatgtctcga
     3721 gaagaataac attacaatga aaaagtgttt ctacatgcaa gcagtgtagg agcacttttt
     3781 ttaatttttg taaaaattaa aaagggtgtt tttgatggaa taaatgaata atattgaaaa
     3841 acaaaaaaat cacgtgctat tgtgaatcaa tcatcgacag gcctatgaaa catcatgtat
     3901 tattatgatt atgtaagtta tttaaataaa aagggtgagt gcatattaat tcgagaatgt
     3961 taaaaacgtc agctgtaatt tctaatacat tattagtcat aggtttagtc tgtctattta
     4021 tgatgaaatt agtgttagct attacttttt ttgctgtttc attatcaatt agtctagttg
     4081 tctttaatat gatgttcaga gatagaacaa cgatgaaagt aatcgttaac gcttcttttt
     4141 taatcgtaat attagcgatt gtagtggcat attttttatt gtctaagtaa gaaaaatgag
     4201 gttttgtcta tatgaagaag aatttcaagt tacgcatttc aacgctacta ttgatagtta
     4261 ttttagttgt ttttgctgta ttactcatcg tgaatgaaac taaattgttt aaaaatgatg
     4321 tgaattactc ttttgatgag gctgtttcaa tgcaacaagg gaaaggtatt gtacagacaa
     4381 aagaagagga tggtaaattt gttgaagcaa ataataatga aattgctaaa gcaatgacta
     4441 tttcacataa agacaatgat atgaagtata tggatatcac agaaaaagtg ccaatgtcgg
     4501 aatctgaagt taaccaattg ctaaaaggta aggggatttt agaaaatcga gggaaagttt
     4561 ttctagaagc tcaagaaaaa tatgaggtta atgtcattta tcttgttagc catgcattag
     4621 tagaaacagg taacggcaaa tcagaattag caaaaggcat taaagatggg aaaaaacgct
     4681 attacaactt ttttggtata ggagcattcg atagtagtgc tgttcgtagt gggaaaagtt
     4741 atgctgaaaa ggaacaatgg acatcaccag ataaggcgat tattggtggt gcaaagttca
     4801 ttcgtaatga atattttgaa aacaatcaac tgaatttata tcaaatgcga tggaatccag
     4861 aaaatcctgc gcaacatcaa tatgcgagtg acattcgatg ggcagataaa attgccaaat
     4921 taatggataa atcctataag cagtttggta taaagaaaga tgatattaga caaacatatt
     4981 ataaataaga catcggtgct taaaggagct ggaacaattt attgtttcga gctcctttag
     5041 cgcattctga gtgtgttagt taaatggatt ttaacctaac aaaaaacgct atatagcatc
     5101 aaatatgcta tatcccacat cattgttaca aatgtacatg atgtaaatga atattgctgt
     5161 ctaaatgtgc atgtaatgta caatggtgca gataatacac ttaagtcctt aaaaatgaaa
     5221 cgttagttcc aagagtcatt tttaaacaat agtgcatgtg ataaaataga aaagaatgaa
     5281 aaatatagag gtgacaatat gaagatagca attataggtg caggcatcgg tggattaaca
     5341 gctgctgcat tattacaaga acaaggtcat actattaaag tctttgaaaa aaatgagtca
     5401 gttaaagaaa ttggcgctgg gattggtatc ggagataatg tgcttaaaaa actaggtaat
     5461 catgacttag ctaaaggtat taaaaatgct gggcaaatct tatctacaat gacagtgtta
     5521 gatgacaaag atcgcctgtt aactactgtt aaattaaaaa gtaatacatt gaatgtgacg
     5581 ttaccacgcc aaacattaat tgacattatt aaatcttatg taaaagatga tgtaatattt
     5641 acaaatcatg aagttacgca tatagataat gagacagata aagttaccat acatttcgcg
     5701 gaacaagaga gtgaagcatt tgatttatgt attggtgctg atggaattca ttctaaagtg
     5761 agacaatctg taaatgctga cagtaaagta ttatatcaag ggtatacatg ctttagaggt
     5821 ttaattgatg atattgattt aaagcatccg gattgtgcaa aagaatactg gggaagaaaa
     5881 ggaagagtag gtattgttcc gttattaaat aatcaagcat attggttcat tacaattaac
     5941 tcgaaggaaa acaatcataa atatagttcg tttggtaaac ctcatttgca agcatacttt
     6001 aatcactatc caaatgaagt tagagagatc ttggacaaac aaagtgaaac aggtatctta
     6061 ttgcataata tttatgattt gaaaccactc aaatcttttg tttatggtcg tactatttta
     6121 ctaggagatg cagcacatgc gacaacgcct aatatggggc aaggtgctgg acaagcaatg
     6181 gaagatgcta tcgtattagt aaattgtttt aacgcatacg actttgaaaa agcattacag
     6241 cgttatgata aaatacgtgt caaacatact gcaaaagtaa ttaagcgttc tagaaaaatc
     6301 ggtaaaattg cccaatatcg tagtcgttta tttgttgcag ttagaaatcg tattatgaaa
     6361 atgatgccaa atgcattagc agctggacaa actaaattct tatataaatc gaaagagaaa
     6421 taatacaaca atatgaaaac ccccgtatgt tgaaacgaga gctcaacata tgggggttct
     6481 tgtttttata atgttattat aataaattca attattagtt aacgacaaat tgtggtttct
     6541 caccttgaac ggcactaatt gcagcattag caacaatttt agacatcatg tcacgtgctt
     6601 caaatgtagc attaccaata tgcggtgtta atactacatt attaagtgat tttaagtcat
     6661 cggtaatatc tggttcaaat tcatatacat caagtgcagc accttcaatt tcattatctt
     6721 tcaatgcttg cactagtgct tgttcgtgca cgattggacc acgagaggca ttgattaaat
     6781 acaccgtaga tttcatcatt ttaaattgtt ctgtatcaat taaatgatgc attttaggat
     6841 tataagcagc gttgatagtg ataaaatctg cattctttaa tagtgtatct aaatctacat
     6901 attttgcacc gatttctcgt tctttttctt ctttgcgatt aggtccagtg tatagcacat
     6961 ccatgtcaaa tgctcttgca cgacgagcta ctgcactacc aatttcacct aaaccgataa
     7021 tgccgattgt tttcccagat acttctctac ctctgaaaaa taaaggtgcc catccgtcaa
     7081 atccagttgt acgtgataat tggtcccctt caacaatacg acgagctact gcaagtacta
     7141 atccaattgt taaatcagca gtcgcgtttg ttgatgcttt aggtgtgttt gtaacatcta
     7201 tacttttttc tcgggcatac tcgatatcaa tattattaaa accagcgcca tagttggcaa
     7261 tgatttttaa gtctttacca gcatcgataa catctttatc aacgtttgta gataataaac
     7321 taattaaggc agtcgcgttt ttaacacctt taattaaagt gtctttatcg actaatcctt
     7381 taccttcata catttcaact tcaaaatgtt cttgtaaaag ttttaaccct acttctggta
     7441 ttgcaccagc aacataaact ttttccataa aagatcactc cttttatctt agtatagtag
     7501 aagattagac agtatacaac tatgtcatga tgtcttgtgt atcaatgatg taagcgcgta
     7561 cttttgatgg aggtgatata acttaggcac tgtagaacta tgaattttgt aatgtggaaa
     7621 aactggatca attaaattag ataacgtagt tttaaa
//
LOCUS       DANBWH010000132         7491 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.136, whole genome shotgun sequence.
ACCESSION   DANBWH010000132 DANBWH010000000
VERSION     DANBWH010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.136"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            138..284
                     /locus_tag="PPY92_001925"
     CDS             138..284
                     /locus_tag="PPY92_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625913.1"
                     /translation="MMLTAIYFSIFTFYISQYSLKLKTLYNIETDYNYKIVLLLKEGE
                     THES"
     gene            274..798
                     /locus_tag="PPY92_001926"
     CDS             274..798
                     /locus_tag="PPY92_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDJ2625914.1"
                     /translation="MNHDKSPIILIGFMGTGKSTIGKYVADEQNLSFIDIDSYIEEKY
                     KLTIPEIFSKHGEQYFRNLEFTCLQECINTADIIATGGGIIESEEAFNFLKNQKNIIW
                     LDCNIDIIYSRINDDPHRPNANNKTIKQLNDLYCSRILRYNEIAFKKFDSHLLSISEI
                     YYELLNLIKASDQY"
     regulatory      785..882
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            957..2048
                     /gene="gcvT"
                     /locus_tag="PPY92_001927"
     CDS             957..2048
                     /gene="gcvT"
                     /locus_tag="PPY92_001927"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HDJ2625915.1"
                     /translation="MSSDLKQTPLYQNYVDRGAKIVEFGGWAMPVQFSSIKEEHNAVR
                     YEIGLFDVSHMGEIEVTGKDASQFVQYLLSNDTDNLTTSKALYTALCNEEGGIIDDLV
                     IYKLADDNYLLVVNAANTEKDFNWILKHKEKFDVEVQNVSNQYGQLAIQGPKARDLIN
                     QLVDEDVTEMKMFEFKQGVKLFGANVILSQSGYTGEDGFEIYCNIDDTEKIWDGLLEY
                     NVMPCGLGARDTLRLEAGLPLHGQDLTESITPYEGGIAFASKPLIDADFIGKSVLKDQ
                     KENGAPRRTVGLELLEKGIARTGYEVMDLDGNIIGEVTSGTQSPSSGKSIALAMIKRD
                     EFEMGRELLVQVRKRQLKAKIVKKNQIDK"
     gene            2068..3414
                     /gene="gcvPA"
                     /locus_tag="PPY92_001928"
     CDS             2068..3414
                     /gene="gcvPA"
                     /locus_tag="PPY92_001928"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HDJ2625916.1"
                     /translation="MSHRYIPLTEKDKQEMLQTIGAKSIGELFGDVPSDILLNRDLNI
                     AEGEAETTLLRRLNRIASKNITKETHTSFLGAGVYDHYAPSVVDAMISRSEFYTAYTP
                     YQPEISQGELQAIFEFQTLICELTDMDVANSSMYDGMTSFAEACILAFSQTKKNKIVV
                     SKGLHYQALQVLHTYAKTRKEFEVVEIDLDGTVTDLKKLEAAVDDETAAVAVQYPNFY
                     GSIEDLEKIQSFIEDKKALFIVYANPLALGLLTPPGSFGADIVVGDTQPFGIPAQFGG
                     PHCGYFATTKKLMRKVPGRLVGQTQDDEGNRGFVLTLQAREQHIRRDKATSNICSNQA
                     LNALASSIAMSALGKQGIYDIAVQNIEHANYAKQQFIKKGFEVLDGTSFNEFVVKFDK
                     PIQQVNEELVKYNIIGGFDLGVVSDDFKNHMLIAVTELRTKDEIDTFVEKAGELND"
     gene            3407..4879
                     /gene="gcvPB"
                     /locus_tag="PPY92_001929"
     CDS             3407..4879
                     /gene="gcvPB"
                     /locus_tag="PPY92_001929"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HDJ2625917.1"
                     /translation="MTSKSSPLIFERSREGRYAYSLPKSDIKTNSVESLLDDKFIRKN
                     KAEFPEVAELDLVRHYTELSNKNFGVDNGFYPLGSCTMKYNPKINEKVARIPGFSESH
                     PLQDEDQVQGSLEIIYSLQEELKEITGMDEVTLQPAAGAHGEWTALMIFKAYHENNGE
                     GHRDEVIVPDSAHGTNPASASFAGFKSVTVKSNERGEVDIDDLKRVVNENTAAIMLTN
                     PNTLGIFEKNIMEIREIVHNAGGLLYYDGANLNAIMDKVRPGDMGFDAVHLNLHKTFT
                     GPHGGGGPGSGPVGVVKELASYLPKPMVIKDGDKFKYDNDIKNSIGRVKPFYGNFGIY
                     LRAYTYIRTMGATGLKEVSEAAVLNANYIKARLSEHFEIPYKQYCKHEFVLSGVRQKE
                     FGVRTLDMAKRLLDFGVHPPTIYFPLNVEEGMMIEPTETESKETLDYFIDTLISIAEE
                     AKNDPDKVLEAPHTTVIDRLDEATAARKPILKFENLKQEK"
     gene            complement(5244..5630)
                     /locus_tag="PPY92_001930"
     CDS             complement(5244..5630)
                     /locus_tag="PPY92_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDJ2625918.1"
                     /translation="MSASLYIAIILVIAIIAYMIVQQILNKRAVKELDQNEFHNGIRK
                     AQVIDVREKVDYDYGHINGSRNIPMTMFRQRFQGLRKDQPVYLCDANGIASYRAARIL
                     KKNGYTDIYMLKGGYKKWTGKIKSKK"
     gene            5788..6618
                     /locus_tag="PPY92_001931"
     CDS             5788..6618
                     /locus_tag="PPY92_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDJ2625919.1"
                     /translation="MTETWNFINTGSKDPYYNMAMDEALLNFVSRGEIDPVIRFYTWN
                     PATLSIGYFQRLQKEIDIDKVKEKGFGLVRRQTGGRGVLHDKELTYSVIVPESHPNMP
                     STVTEAYRVISQGLLEGFKNLGFDTYFAVPKTPEERQKLKQPRSSVCFDAPSWYELVV
                     EGRKIAGSAQTRQKGVILQHGSILQDIDIDELFDMFIYKNERLKLKMKEAFVEKAVAI
                     NDISDEHITISQMEEAFEKGFKKGLNIELKPLELTEAQLAEVEELTEKYRSDEWMFRK
                     "
     gene            complement(6682..6900)
                     /locus_tag="PPY92_001932"
     CDS             complement(6682..6900)
                     /locus_tag="PPY92_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625920.1"
                     /translation="MRNIIFYLVLIIAAIGLVMNLDAFIFSIVRMLISFAVIAGIIYL
                     IYYFFILTEDQRKYRKAMRKYKRNQRRK"
     gene            complement(6914..7486)
                     /locus_tag="PPY92_001933"
     CDS             complement(6914..7486)
                     /locus_tag="PPY92_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625921.1"
                     /translation="MVSIVGATLLLAGCGSQNLAPLEEKTTDLREDNHQLKLDIQELN
                     QQISDSKSKIKGLEKDKENSKKTASNNTKIKLMNVTSTYYDKVAKALKSYNDIEKDVS
                     KNKGDKNVQSKLNQISNDIQSAHTSYKDAIDGLSLSDDDKKTSKNIDKLNSDLNHAFD
                     DIKNGYQNKDKKQLTKGQQALSKLNLNAKS"
BASE COUNT         2638 a         1013 c         1366 g         2474 t
ORIGIN      
        1 aagtcggtac aaatctgcag actaaaacta tttatgtata acttactaga atttaaatga
       61 gtactattaa aaaggatgtg aaataattta ttaattttga tttatataaa gaaaaaggat
      121 atgtaactcc gttcttattg atgctaacag caatttattt ttccattttc actttttata
      181 tcagtcaata tagtttaaaa ttaaagacat tatataatat tgaaactgac tataactata
      241 agattgtttt gttattgaaa gaaggggaaa cgcatgaatc atgataaatc accaataatt
      301 ttaattggtt tcatgggtac cggtaaatct acgattggta aatacgttgc agatgagcaa
      361 aatttatcat ttattgatat agattcatat atcgaagaga agtataagtt aacaatacca
      421 gaaatattta gtaaacatgg tgaacaatat ttcaggaatt tagagttcac atgtttgcaa
      481 gaatgtatta acactgcaga tataattgct actggtggtg gtattattga aagtgaagag
      541 gcatttaatt ttttgaaaaa tcaaaaaaac attatttggt tagattgtaa tattgatatt
      601 atatatagtc gaatcaatga tgacccacat cgacctaatg caaataataa gacaatcaag
      661 cagttaaatg acttgtattg ctcgcggatt ttaagatata atgaaatcgc attcaagaaa
      721 tttgatagtc atttgctatc aatttcagaa atatattatg aattgctaaa tttaataaaa
      781 gcgagtgatc agtattagag agaatagagc gttaagactc tatcgccgaa ggtgcaagta
      841 atttattacg aaactctcag gcaaaaggat aatactgtaa cgcgttcctg aattggtgat
      901 ttataaacag ggtagcgatt gctatcctgt ttttataatt ttaagggggt atttcaatgt
      961 caagtgattt aaaacaaaca cctttatatc aaaattatgt tgatagaggt gcaaaaattg
     1021 tggaattcgg aggatgggcg atgcctgttc aattttcaag tattaaagag gagcataatg
     1081 ctgttcgata cgaaattggc ctgtttgatg ttagtcatat gggtgaaatt gaagtaacag
     1141 gtaaagatgc tagtcagttt gtgcaatatt tattatcaaa tgatactgat aatttaacta
     1201 cttcaaaagc attatatact gctttatgta atgaagaagg cggtattatt gatgatttag
     1261 taatatataa attagctgac gacaattatt tattagttgt taatgctgct aatactgaaa
     1321 aagattttaa ttggatttta aaacacaaag agaaatttga tgttgaagta caaaatgtat
     1381 caaaccaata tggtcaatta gcaatacaag gaccaaaagc tagagattta attaatcaat
     1441 tagttgatga agatgtaact gaaatgaaaa tgtttgaatt taaacagggt gtcaaattat
     1501 ttggagcaaa cgtcatttta tctcagtcag gttacacagg tgaagatggg tttgaaattt
     1561 attgtaatat cgatgatact gaaaaaattt gggatggttt attagagtat aatgttatgc
     1621 catgtgggtt aggcgctcgt gatacattaa gattagaggc tggattgcca ttacatggac
     1681 aagatttaac tgaatcaatt acaccatatg aaggtggtat cgcttttgca agtaaaccat
     1741 taattgatgc tgattttatt ggtaaatctg tattaaaaga tcaaaaagaa aatggtgcac
     1801 caagaagaac agtgggatta gaattacttg aaaaaggaat tgcaagaact ggttatgaag
     1861 ttatggattt agatggaaat attattggag aagtaacttc aggaacacag tctccatcat
     1921 caggaaaatc aattgcactt gcaatgataa aaagagatga gtttgaaatg ggtagagagt
     1981 tgcttgttca agttcgtaag cgtcaattaa aagcgaaaat tgttaagaaa aatcaaattg
     2041 ataaataatt aaaaaggggt gtgcattgtg agtcatcgtt atataccttt aactgaaaaa
     2101 gacaagcaag aaatgttaca aacaattggt gcaaaatcta taggagaatt attcggtgat
     2161 gtaccaagtg acattttatt aaatagagat ttaaatattg ctgaaggcga agcggaaaca
     2221 acgttactta gaagattaaa tcgcattgca agcaagaata tcactaaaga aacgcataca
     2281 tcatttttgg gtgctggtgt ttatgatcat tacgcgccat cagttgtgga tgctatgatt
     2341 tcaagatcag aattttacac agcatatacg ccatatcaac cggaaatttc tcaaggtgaa
     2401 ttacaagcca tttttgagtt ccaaacttta atttgtgaat taacagatat ggatgtagca
     2461 aattcttcta tgtatgatgg gatgactagc tttgctgaag catgtatctt agcatttagt
     2521 caaacaaaga aaaataaaat tgtagtctca aaaggtttac attatcaagc attacaagta
     2581 cttcatactt atgctaagac gcgtaaggaa ttcgaagtag tggaaatcga tttagatggc
     2641 actgttacag atttgaaaaa attagaagca gcagtggatg atgaaacagc agctgttgca
     2701 gtacaatatc caaactttta cggttcaatt gaagatcttg aaaagattca aagctttatt
     2761 gaagataaaa aagcattatt tatcgtatat gcaaatccat tagcattggg attacttaca
     2821 cctccaggtt catttggtgc agatattgtt gtaggtgata ctcagccatt cggaattcct
     2881 gctcaattcg gtgggccaca ttgtggatat tttgcaacga cgaaaaagtt aatgcgtaaa
     2941 gtgccaggta gattagtcgg tcaaacacaa gatgatgaag gtaatcgtgg ctttgtatta
     3001 acattacaag cacgtgaaca acatattcga cgcgataaag cgacatctaa tatttgttct
     3061 aaccaagcat taaatgcttt agcgtcatca attgcaatgt cagcacttgg taaacaaggt
     3121 atctatgata ttgcagttca aaatatagaa catgctaact atgctaaaca acaatttatt
     3181 aaaaaaggtt ttgaagtatt agatggtaca tctttcaatg agtttgtagt caaatttgac
     3241 aaaccaattc aacaagttaa cgaggaatta gtgaagtaca atattatcgg tggttttgat
     3301 ttaggtgttg tttcagacga ctttaaaaat cacatgttaa tagctgtaac tgaattaaga
     3361 acaaaagatg aaatcgatac atttgtagaa aaggctggtg agttaaatga ctagtaaatc
     3421 aagtccatta atttttgaaa gatctcgtga aggcagatat gcatattcat taccaaaaag
     3481 tgatattaaa acaaattctg ttgagtcatt gttagatgat aaatttattc gtaaaaacaa
     3541 agcggagttt cctgaagttg ctgaactaga cttagtacgt cattatactg aactttcaaa
     3601 taaaaatttc ggtgtagaca acggatttta tccattgggt tcatgtacga tgaagtataa
     3661 tcctaaaatt aatgaaaagg ttgctagaat accaggattc agtgaatcac atccattaca
     3721 agatgaagac caagttcaag gttcattaga aattatttat agtttgcaag aagaattaaa
     3781 agaaattact ggtatggatg aggtaacatt acaaccagct gctggtgcac atggtgaatg
     3841 gactgcattg atgatattta aagcttatca tgagaataat ggtgaaggtc atcgtgatga
     3901 agtcattgtg ccagattctg cgcatggtac gaatccagcc tcagcttcat ttgcaggatt
     3961 taaatcagtt actgtaaaat caaacgaacg tggcgaagtt gatattgatg acttgaaacg
     4021 tgttgtaaat gaaaatacag cagctattat gttaactaat ccaaatactt taggtatttt
     4081 cgaaaaaaac attatggaaa tccgtgaaat cgtccataat gctggtggtc tattatatta
     4141 tgatggtgcg aatttaaacg ctattatgga caaagttcgc ccaggagata tgggatttga
     4201 tgctgttcat ttaaacttgc ataaaacatt tactggtcca catggtggcg gcggtcctgg
     4261 ttcaggtcca gtcggtgtag taaaagaact agcaagttac ttaccaaagc caatggttat
     4321 taaagatggc gacaaattta aatatgataa tgacattaaa aattctatcg gacgtgtaaa
     4381 accattttat ggtaactttg gtatttactt aagagcttat acgtatattc gaactatggg
     4441 agcaactgga cttaaagagg tttcggaagc agcggttctt aatgcgaatt atattaaagc
     4501 acgtttatct gaacactttg aaatacctta taaacaatat tgtaaacatg agtttgtgtt
     4561 aagtggtgtg cgtcaaaaag aatttggtgt acgtacttta gacatggcta agcgattatt
     4621 agatttcggt gtacatccac caacaatata cttcccatta aatgttgaag aaggtatgat
     4681 gattgaaccg actgagacag agtctaaaga aacacttgat tattttatcg atacattaat
     4741 tagtattgct gaagaagcta aaaatgatcc tgataaagtg ctagaagcac cacatacaac
     4801 tgtgattgat cgattagacg aagctacagc tgctcgtaaa ccaatattaa agtttgaaaa
     4861 tcttaaacag gaaaaataaa gtattaaaca catattccga gaattattat tctaactttg
     4921 tatgaagatt taaggataat ggtttcaaaa tcaattgaaa aagacaattt ctatttgaac
     4981 aagaaaaata aaccgaagta ataactctta gggtttggta ttattctttc atagaaattg
     5041 tcttttcatt ttttagattg cggtaattga atcgtattga aaatgagctg aactttcttt
     5101 attatgctga aactaagttt aatgatcagt gaagctagag aaatatagta acaggctttg
     5161 ttaaaaaatt aaaatctgag tattgttatt gatgatcaat taacactcag attttattaa
     5221 atcgtatatt aaatttacaa aaactatttt ttagacttta tttttccagt ccatttttta
     5281 tagccgcctt ttaacatata gatatctgta tatccattct ttttcaaaat acgagcggct
     5341 ctatagctag caatcccatt ggcatcacat aagtataccg gttgatcttt tcttaatcct
     5401 tggaatcgtt gcctgaacat tgtcatagga atattgcgag acccattaat gtgaccgtag
     5461 tcatagtcaa ctttctctct aacatcgatg acttgagctt ttctaatccc attatggaat
     5521 tcattttgat ctaattcttt aacagctcgc ttgttaagaa tttgttgaac aatcatataa
     5581 gcaataattg ctataactaa aattattgcg atgtacaaac tagcactcat cttgcatcct
     5641 ccctaattaa acgataatat aattataaga ctgttaggtc aaattatcaa aatatttcac
     5701 taaagaattg caattattat tcatttagtt aaattgatta attagcgaat caaatattat
     5761 acattttgct gataggagtt cttgaatatg actgaaactt ggaattttat taatacagga
     5821 agtaaagacc catattataa tatggcaatg gatgaagcgt tattaaattt tgtctctaga
     5881 ggtgaaattg atccagttat acgtttttac acatggaatc ctgcaacatt atcaataggg
     5941 tatttccaaa ggctacaaaa agaaattgat attgataagg ttaaagagaa aggttttggt
     6001 ctcgtaaggc gtcaaactgg tggccgtggg gtgctacatg ataaagagtt aacttacagt
     6061 gtgattgtgc ctgaatcgca tccgaatatg ccttctacag ttacagaagc gtacagagtg
     6121 atttcacaag gattattaga aggatttaaa aacttaggat ttgatactta ttttgcggta
     6181 cctaaaacgc ctgaagagcg tcaaaaatta aaacaacctc gaagttcagt gtgttttgat
     6241 gcaccaagtt ggtatgagct agttgttgaa ggccgtaaga ttgctgggag tgctcaaacg
     6301 agacaaaagg gcgtaatcct ccaacacggt tcgattttac aagatattga tattgatgaa
     6361 ctgtttgata tgtttattta taaaaatgaa agattaaaat taaaaatgaa agaggcattc
     6421 gttgaaaaag cagttgccat caatgatatt tcagatgaac acattactat tagtcaaatg
     6481 gaagaagctt tcgagaaagg ttttaagaaa ggtttaaata tagaattaaa gccacttgaa
     6541 ttaacagaag ctcaattggc agaagtggaa gaattaactg aaaaatatcg ttcagatgaa
     6601 tggatgttta gaaagtagtt tttagaataa aaaatggaag cacgtagact attttaccta
     6661 caagtgcttc cgttttttta tctattttct tctttgattt cttttatact tacgcattgc
     6721 tttgcgatat ttgcgttggt cttcagttaa gatgaagaaa taataaatca gataaataat
     6781 accagctatt acagcaaagc tgattaacat tctgacgatt gaaaaaataa aggcatctag
     6841 attcattact aatccaatcg cagcaataat aagtacaaga taaaatatta tattacgcat
     6901 taaaagactc ctatcatgat tttgcattta agtttaattt tgacaacgct tgttgtcctt
     6961 ttgtaagttg ttttttatct ttattttgat agccattttt aatatcatca aatgcatgat
     7021 tcaaatcaga gtttaattta tcgatatttt tagacgtttt tttatcatca tcactaagtg
     7081 ataaaccatc gatagcatct ttgtatgaag tgtgagcact ttgaatatca ttagaaattt
     7141 gatttaattt cgattgaaca ttcttatcgc ctttgttttt acttacatct ttctcaatat
     7201 cgttatagga tttcaaagct ttagcaactt tgtcgtagta tgttgatgta acattcatca
     7261 atttaatttt cgtattatta gatgcagttt ttttactgtt ttctttatcc ttttcaagcc
     7321 ctttaatttt agatttagaa tcactaattt gttgattaag ttcttgaata tctagtttga
     7381 gttgatgatt atcttctctt aaatctgttg ttttttcttc taatggtgct aaattttgtg
     7441 atccacatcc agctaacaat aatgttgcgc caacaattga aaccaatttt t
//
LOCUS       DANBWH010000133         7394 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.137, whole genome shotgun sequence.
ACCESSION   DANBWH010000133 DANBWH010000000
VERSION     DANBWH010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7394
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.137"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            33..1490
                     /locus_tag="PPY92_001934"
     CDS             33..1490
                     /locus_tag="PPY92_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500488.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDJ2625922.1"
                     /translation="MKCLIRFILVLGLLISSAMVYINPTAHAEQDQTWEKIKERGELR
                     VGLSADYAPMEFEHTVNGKTEYAGVDIDLAKKIAKDNNLKLKIVNMSFDSLLGALKTG
                     KIDIIISGMTSTPERKKQVDFSDSYMMTKNIMLVKKDKVNEYKDIKDFNNKKVGAQKG
                     TEQEKIAQTEIENASITSLSRLPDVILALKSGKVEGAVVEKPVAEAYLKQNPKLGISN
                     VKFNEEEKDTVIAVPKDSPKLLSQINKTIKEVKDKGLIDKYMTNAANAMNDDSGFISK
                     YGSFFLKGIKITILISLIGVALGSILGAFVALMKLSKIKIISWIASIYIEILRGTPML
                     VQVFIVFFGITAALGLDISALVCGTIALVINSSAYIAEIIRAGINAVDKGQMEAARSL
                     GLNYRQTMKSVIMPQAIKNILPALGNEFVTLIKESSIVSTIGVGEIMFNAQVVQGISF
                     DPFTPLLVAAALYFVLTFVLTRIMNMIEGRLNASD"
     gene            1483..2205
                     /locus_tag="PPY92_001935"
     CDS             1483..2205
                     /locus_tag="PPY92_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDJ2625923.1"
                     /translation="MIKINNLNKVFGDNEVLKDINLEINQGEVVAIIGPSGSGKSTLL
                     RCMNLLEVPTKGQVIFEGNDLTEKGTQVDKLRQKMGMVFQNFNLFPHKKVVDNIILAP
                     KLLKKDNNDELHKEALSLLDKVGLKEKADVYPNQLSGGQKQRVAIARALAMHPDVILF
                     DEPTSALDPEVVGDVLKVMKDLAKEGMTMVVVTHEMGFAKDVSDKVIFMADGVVVESG
                     TPVEIFEQPQHERTQNFLARVL"
     gene            2356..3483
                     /gene="queG"
                     /locus_tag="PPY92_001936"
     CDS             2356..3483
                     /gene="queG"
                     /locus_tag="PPY92_001936"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDJ2625924.1"
                     /translation="MDTKQLKQDIIDYAYTIGIDSIGFTTADPFDELKQKLEAYHANG
                     YASGFEESDIALRTEPKLSLPTARSIIAIAVGYPNKLKGAPKSVRGDRRGLFARASWG
                     QDYHTIMRKRLDMLAAFIESKVPDVEIKSMVDTGVLSDRAVAERAGLGFVGRNGFVIN
                     PKLGTWTYLGEMLVSIPFEPDDPLLDSCGDCTICVDRCPTSALVGNGQLNSQKCISFL
                     TQTKGYMPDQYRYKIGNRLYGCDTCQQVCPKNRGINTEQDDIILEPEILKPRLVPLLR
                     MSNKEFKQTYGHLAGAWRGKKPIQRNAILALAHFNEVDAIPELKKVATTDERPMIRAT
                     AYWAIGQILGEEARDFINANYDQEDAEVQNEMIKGLDTRRE"
     gene            3488..3958
                     /gene="trmL"
                     /locus_tag="PPY92_001937"
     CDS             3488..3958
                     /gene="trmL"
                     /locus_tag="PPY92_001937"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDJ2625925.1"
                     /translation="MTNHIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKM
                     LKRAGLDYWEFVNITYHDSIEAFFEATNGEYYLLTKFGKKTYSDFDFSNHDKDFYFIF
                     GKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGLN"
     gene            4117..4716
                     /locus_tag="PPY92_001938"
     CDS             4117..4716
                     /locus_tag="PPY92_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HDJ2625926.1"
                     /translation="MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAP
                     VLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGVPAGQVYPIAYEKDAIELIADKI
                     KTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
                     TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA"
     gene            4741..4893
                     /locus_tag="PPY92_001939"
     CDS             4741..4893
                     /locus_tag="PPY92_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625927.1"
                     /translation="MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKN
                     SEEDSQ"
     gene            5441..5836
                     /locus_tag="PPY92_001940"
     CDS             5441..5836
                     /locus_tag="PPY92_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625928.1"
                     /translation="MLGKYWIQLLIATVIISLISIKAFPLAIGALYLPVIFKVIKLQL
                     NLSKGLIDDVNAQTFIKSNQSGIVISVICCLLITGILYYTLDGFYASLTGVLGTLVAL
                     NPYTTIVSAVLYILTAIATVEATKTKYRN"
     gene            6032..>7394
                     /gene="fumC"
                     /locus_tag="PPY92_001941"
     CDS             6032..>7394
                     /gene="fumC"
                     /locus_tag="PPY92_001941"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDJ2625929.1"
                     /translation="MSVRIEHDTFGEIEVPADKYWGAQTERSKRNFPVGKERMPIEVV
                     YGFAQLKRAAALANFDLGKLSEAKKDAIVYACDQILSGELDEHFPLVVWQTGSGTQSN
                     MNVNEVVSYVANMYLKDHQIDESIHPNDDVNESQSSNDTFPTAMHVALYQEVETKLEP
                     ALKLLRNTLKEKEDKFESIIKIGRTHLQDATPIKLGQEISGWRYMLDRCEIMLSESKK
                     HILNLAIGGTAVGTGINAHPEFGDKVAHYISENTGYPFVSSENKFHALTAHDEVVQLH
                     GTLKALAGDLMKIANDVRWLASGPRAGLAEISIPENEPGSSIMPGKVNPTQCEMLTMV
                     AVQVMGNDTVVGFASSQGNFELNVYKPVIMHNTLQSIYLLADGMETFNNNCAVGIEPI
                     EENIDNYLNQSLMLVTALNPHIGYEKAAQIAKKAHKEGLTLKESAIQTGYVTEEQFEA
                     WIKP"
BASE COUNT         2710 a          993 c         1403 g         2288 t
ORIGIN      
        1 attatgaata ttaattaggg agggctgtat aaatgaagtg tttaattagg tttatattag
       61 tattagggtt gttaattagc agtgctatgg tgtatatcaa tcccacagca catgctgaac
      121 aagatcaaac atgggagaag attaaagaac gcggtgaact tagagtgggt ctttctgcag
      181 attatgcacc aatggaattt gagcatacag ttaatggtaa gactgagtat gcaggtgtag
      241 atattgattt agctaaaaaa attgcgaaag ataataattt aaaattaaaa atcgtcaata
      301 tgtcatttga tagtttgtta ggagctctta aaactggaaa aattgatatt attatttccg
      361 gaatgacttc aacgcctgaa cgtaagaagc aagttgattt ttcagattca tatatgatga
      421 ctaaaaatat catgcttgta aagaaagata aagttaatga atataaagat atcaaagact
      481 ttaataataa aaaagtaggg gcacaaaagg gaactgaaca agaaaaaatc gctcaaaccg
      541 aaattgaaaa tgcatctatt acttcattaa gccgtttacc agacgttata ttagcattga
      601 aaagtggaaa ggttgaaggt gctgtagttg aaaaacctgt agcagaagca tatttaaaac
      661 aaaatcctaa attaggaatt tccaatgtga aatttaatga agaagaaaaa gatacagtga
      721 tagctgtgcc aaaagattca ccaaaattat tgtcacaaat taataaaacg attaaagagg
      781 ttaaagataa aggattaatc gataaatata tgactaatgc tgcaaatgcg atgaatgatg
      841 acagtggttt tatttctaag tatggtagtt ttttcttgaa aggaattaag attacaatat
      901 taatttcact tatcggtgtt gcattaggtt ctattttagg tgcattcgtt gcgttaatga
      961 aattaagtaa aataaaaatt atttcatgga ttgcttctat ctatatcgaa atattaagag
     1021 gaacaccaat gttagttcaa gtatttatcg tattctttgg tataactgct gcattaggac
     1081 tagacatttc agctcttgtt tgtggaacaa ttgcattagt tattaattcc tcagcttaca
     1141 ttgctgaaat tattcgtgca ggtataaatg ctgttgataa aggacaaatg gaagccgcac
     1201 gtagtctagg tttgaattat agacaaacga tgaaaagtgt aattatgcca caagcaatta
     1261 aaaatatttt accagcttta ggtaatgaat ttgtcacttt aattaaagaa tcatctattg
     1321 tgtcaacaat tggagttggc gaaattatgt ttaatgcaca agtggttcaa ggtatttcat
     1381 ttgacccatt cacaccatta ctagtggcag cagcattata ctttgtctta acatttgtac
     1441 ttacacgcat tatgaacatg attgaaggga gattgaatgc cagtgattaa aataaacaat
     1501 cttaataaag tttttggaga taatgaagtt ttaaaagata tcaatcttga aatcaatcaa
     1561 ggggaagtag tagcaataat aggtccatct ggtagtggta aaagtacatt gttaagatgt
     1621 atgaatttat tagaagtacc cactaaaggt caagtgattt ttgaaggcaa tgacttaacg
     1681 gaaaaaggga cacaagtaga taaactacgt caaaaaatgg gtatggtatt tcaaaacttc
     1741 aacctatttc cacataaaaa agttgtcgat aatattattt tagctcctaa attattaaag
     1801 aaagataata acgatgaatt acataaggaa gcattgtcgt tattagataa agtgggatta
     1861 aaagaaaaag cagatgtata tccgaatcaa ttatcaggtg gtcaaaagca aagggttgca
     1921 attgcaagag ctttagcaat gcatccagat gttattttat tcgatgaacc aacttcagca
     1981 ttagatcctg aggtagttgg tgatgtatta aaagtaatga aagacctagc caaagaaggt
     2041 atgaccatgg tggttgtgac acatgaaatg ggatttgcca aagatgtaag tgacaaagtc
     2101 atatttatgg cagatggcgt tgtcgtagag tcaggcacac cagtcgaaat atttgaacaa
     2161 ccgcaacatg aaagaacaca aaatttctta gcaagagtat tataacaacc taacgaagct
     2221 tgaatatatg atgcgcacca caaagttata tcatatattc aagcttttta ttattaaaat
     2281 tatgtgaaat gaaacctcaa tatgaataat ggttgtgtta aaataacata gtattatttt
     2341 atggggggca tacgattgga tacaaagcag ttaaagcaag atatcattga ctatgcatat
     2401 acaattggta tcgacagtat tggatttaca actgccgatc cctttgatga attgaagcaa
     2461 aagctagaag catatcatgc aaatggttat gcctcaggat ttgaagaatc tgatattgct
     2521 ttacgaacgg agcctaaatt atccttacca acagcaaggt caatcatagc aattgcagtt
     2581 ggttatccta acaaactgaa aggtgcacct aagagtgtta gaggtgatcg cagaggctta
     2641 tttgctagag catcgtgggg tcaagattat catacaatta tgcgtaaacg attagacatg
     2701 ttagctgcat ttattgaatc taaagttcca gatgttgaaa tcaaatctat ggtagatacg
     2761 ggtgtattat cagatagggc agtagcagaa cgtgcaggtt taggatttgt tggtcgtaat
     2821 ggctttgtca tcaatcctaa actaggaaca tggacatacc tcggtgaaat gttagtcagt
     2881 ataccttttg aacctgatga tccattatta gatagctgtg gtgattgtac aatttgtgtt
     2941 gatcgttgtc caacaagtgc attagttgga aatggccaat taaatagtca gaaatgcatt
     3001 agttttttaa cgcaaacaaa aggctatatg cctgaccaat atcgttataa aattgggaat
     3061 agattatatg gttgcgatac gtgtcaacaa gtttgtccga aaaatagagg tattaatacc
     3121 gaacaagatg acatcatttt ggaaccagaa attttaaagc caagattagt acctttacta
     3181 cgtatgtcta ataaagaatt caaacaaaca tatggtcacc ttgcaggtgc ttggcgtggt
     3241 aaaaagccga tacaacgaaa tgctatttta gcattagcgc attttaatga agtggatgca
     3301 attccagaat tgaaaaaagt tgcaacaaca gatgaaagac cgatgattcg agcaactgct
     3361 tattgggcca ttggtcaaat tcttggggaa gaagcaagag attttatcaa tgctaattat
     3421 gatcaagagg atgcagaagt tcagaatgaa atgataaaag gattagacac aaggagagaa
     3481 tagtaatatg acaaatcata tcgttttata tcaaccagaa atcccagcta atactggtaa
     3541 cattgcacgt acgtgtgcag gtacaaacac acatttacat ttaattaaac ctcttggatt
     3601 tagaactgat gataaaatgt taaaaagagc cggtttagat tattgggaat ttgtgaatat
     3661 tacgtatcat gatagtattg aagcattttt tgaagccaca aatggcgagt attacttatt
     3721 aacaaaattt ggtaaaaaga cttatagtga ttttgatttt tcaaatcatg acaaagattt
     3781 ttacttcatt tttggaaaag agacgacagg attaccagac tgggtgaaag agaaatatca
     3841 agacacagcg ttaagaattc ctatgagtga acatattcgt tcattaaatt tatcaaatac
     3901 tgctgcgttg ttaatttatg aagcgttacg tcaacaagat tttccaggat tgaattaaaa
     3961 ttcgtctatt ttcattataa aaatgatact taagtgctat atttagagta taaccatgtt
     4021 aaagttaaat ggtttgatat attgaaagtt agggtataat aattattaat atcgccgttt
     4081 ttatgtaaaa tgcaaactaa gaggagtgta gaaatcatgg caatgaactt taaagtcttt
     4141 gacaatagtc aacttgtagc agaatatgct gctgatatta ttagaaagca atttaacaat
     4201 aatcctacta caattgcagg ttttcattta gatacagatc aagcgccagt tctagatgaa
     4261 ttaaagaaaa atgttgaaaa acatgctgtt gattttagcc aaataaatat tttagattat
     4321 gacgataaaa aatcatattt cgaagcgtta ggtgtaccag caggtcaagt ttatccaatt
     4381 gcttatgaaa aagatgcaat cgaattaatc gctgataaga ttaaaactaa agaaaataaa
     4441 gggaaattaa cattacaagt tgtttctatc gatgagcaag gtaagttaaa tgttagtatt
     4501 cgtcaaggac taatggaagc aagagaaatt ttcttagtag tgacaggtgc taataaacga
     4561 gatgtagttg aaaaattata tcaagaaaat ggtaaaacaa gcttcgaacc agccgattta
     4621 aaagcacata gaatggtaaa tgttattctt gataaagaag cggctgcagg tttacctgaa
     4681 gatgttaaag cttactttac gtcacgcttt gcttaattaa aaatctgtga ggtattgatt
     4741 atgtctaaag acaaagatcc aaaattaaat tatcatgaag aagaaaacag tatggtaacg
     4801 gattttgaag atttaaaaga attaggtaaa gaaatggaac aaatctctga tcaaaatgat
     4861 caagaaaaaa attctgaaga agacagtcag taaagtatta taattgtgta atattcagtt
     4921 atttatcatg taaataatgt atagtataga acatatatca aataggattg attatcttga
     4981 atatgaagtt aggtagcatt ttgttaccta gcttttttat tatattcata gaagctgact
     5041 ttctgccagc ttctgagttt gtggcccgtt tccccaactt gcactgtttg tagaaattag
     5101 aaatccgatt tcactatgtt ggggcctcgc caacttgcat gaaatagtaa gctgactttc
     5161 caccagcttc tatgtttgtg gactgcagtt aactactgcc aatataactt tgtaaagcat
     5221 aaaacattga tttgtgtacc agacatagta atatatcaaa agttcaattt taaatattaa
     5281 aacaccattt aaacggaatt gataattaat tactccagac ataattgaaa gaagtcatct
     5341 atcagtaata tttttaatga aatcattaaa ccgcgtttct taatatgcca agattatgat
     5401 atgctattga agaatgaaat tagaggagat ttatatattt atgttaggaa aatactggat
     5461 tcaattattg atagcaacag ttattataag tttaatatca attaaagctt ttccattggc
     5521 aattggggca ttatatttac ctgttatttt caaagtaata aaattacaac tcaatttatc
     5581 aaaaggatta atcgatgatg ttaatgcgca aacattcatt aaaagtaatc aatcaggtat
     5641 tgtaataagt gtaatttgtt gtttattaat aacaggaata ttgtattata cacttgatgg
     5701 gttttatgcg agtttaacag gagttttagg tacgcttgtt gctctaaatc catatacgac
     5761 aattgttagt gctgtattat atatacttac agctattgct acggttgaag ctactaaaac
     5821 aaagtatcgt aattaaactt agttcaacaa taactaaata atgatagata acacactaat
     5881 cgtatgaccg tatcactgta ataaagggtg agtacgtgcg attagtgtat ttttatttca
     5941 aattatgaaa acgttttaat cataaattgc tcaattaaat tatttaatga tatactacag
     6001 ttaaataaac agtgataagg ggagaaattg aatgtcagta agaattgaac atgatacttt
     6061 tggagaaata gaagtacctg cagataaata ttggggtgct caaacagaaa gaagtaaacg
     6121 caatttccca gttggtaaag agcgtatgcc aatcgaagta gtttatggtt ttgcacaact
     6181 aaagcgtgca gcagcattag ctaattttga tttaggaaaa ttaagcgagg caaagaaaga
     6241 tgccattgta tacgcatgtg atcaaatttt atcaggtgaa ttagatgaac acttcccact
     6301 agttgtatgg caaacaggaa gcggtacaca aagtaatatg aatgtgaacg aagtagtaag
     6361 ttatgttgct aatatgtatt taaaagatca tcaaattgat gaaagtatcc acccaaatga
     6421 tgatgtaaat gaatcacaaa gttcgaatga tacattccca actgctatgc acgttgcatt
     6481 atatcaagag gttgaaacaa aattagaacc tgcattaaaa cttttaagaa atactttgaa
     6541 agaaaaagaa gacaaatttg aatcaattat taaaattggt cgtacacatt tacaagatgc
     6601 aacgccgatc aaactaggac aagagattag tggctggcgt tatatgcttg accgttgcga
     6661 aataatgtta tctgaatcta agaagcacat tttaaatctt gccatcggtg gtacggctgt
     6721 tggtactggt attaatgcgc atcctgaatt tggtgataag gtggcacatt atatttcaga
     6781 aaatacgggt tatccatttg tatcttctga aaataaattc cacgcactta cagcgcatga
     6841 tgaagttgtt caattgcacg gaacattgaa ggcattagct ggagatttaa tgaaaattgc
     6901 taatgatgtg agatggttgg cttcaggtcc acgagctggt ttggcagaaa tttccatccc
     6961 tgaaaatgaa ccaggttcat caattatgcc tggtaaagtt aatcctacac aatgtgaaat
     7021 gttaacaatg gttgcagtcc aagtaatggg taatgataca gttgttggct tcgcaagttc
     7081 acaaggtaac tttgaattga atgtttataa accagttatt atgcataata cactacaatc
     7141 aatttacctt ttagctgatg gtatggaaac atttaataac aattgtgcag tgggcattga
     7201 gccaatcgaa gagaatattg ataattattt aaatcaatca ttaatgttag ttactgcatt
     7261 aaatccacat attggttatg aaaaagcagc tcaaattgct aagaaagccc ataaagaagg
     7321 tttaacttta aaagaatctg caattcaaac tggatatgtt acagaagaac aatttgaagc
     7381 atggattaaa ccag
//
LOCUS       DANBWH010000134         7346 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.138, whole genome shotgun sequence.
ACCESSION   DANBWH010000134 DANBWH010000000
VERSION     DANBWH010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7346
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.138"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            152..1135
                     /locus_tag="PPY92_001942"
     CDS             152..1135
                     /locus_tag="PPY92_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDJ2625930.1"
                     /translation="MKKWQFVGTTALGATLLLGACGGGNGGSGNSDLKGEAKGDGSST
                     VAPIVEKLNEKWAQDHSDAKISAGQAGTGAGFQKFIAGDIDFADASRPIKDEEKQKLQ
                     DKNIKYKEFKIAQDGVTVAVNKENDFVDELDKQQLKAIYSGKAKTWKDVNSKWPDKKI
                     NAVSPNSSHGTYDFFENEVMNKEDIKAEKNADTNAIVSSVTKNKEGIGYFGYNFYVQN
                     KDKLKEVKIKDENGKATEPTKKTIQDNSYALSRPLFIYVNEKALKDNKVMSEFIKFVL
                     EDKGKAAEEAGYVAAPEKTYKSQLDDLKAFIDKNQKSDDKKSDDKKSEDKK"
     gene            1326..2252
                     /gene="pstC"
                     /locus_tag="PPY92_001943"
     CDS             1326..2252
                     /gene="pstC"
                     /locus_tag="PPY92_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDJ2625931.1"
                     /translation="MTSSTNVKALIEKNINKKGKHNDKIIPVILAAISAISILTTLGI
                     LITLLLETITFFTRIPITEFLFSTTWNPTGSDPKFGIWALIIGTLKITVIATIFAVPV
                     GLGAAIYLSEYASDRARRIIKPILEILAGIPTIVFGFFALTFVTPVLRSFISGLGEFN
                     AISPGLVVGIMIVPLITSLSEDAMASVPNKIREGAYGLGATKLEVATKVVLPAATSGI
                     VASIVLAISRAIGETMIVSLAAGSSPTASLSLTSSIQTMTGYIVEIATGDATFGSNIY
                     YSIYAVGFTLFIFTLIMNLLSQWISKRFREEY"
     gene            2254..3171
                     /gene="pstA"
                     /locus_tag="PPY92_001944"
     CDS             2254..3171
                     /gene="pstA"
                     /locus_tag="PPY92_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDJ2625932.1"
                     /translation="METTDNNRQSLVDQQLVQKHLSSRTVKNKVFKLIFLACTLLGLV
                     VLIALLTQTLIKGVSHLNLQFFTNFSSSTPSMAGVKGALIGSLWLMLSIIPLSIILGI
                     GTAIYLEEYAKNNKFTQFVKISISNLAGVPSVVFGLLGYTLFVGGAGIEALKMGNSIL
                     AAALTMTLLILPIIIVSSQEAIRAVPNSVREASYGLGANKWQTIRRVVLPAALPGILT
                     GFILSLSRALGETAPLVLIGIPTILLATPRSILDQFSALPIQIFTWAKMPQEEFQNVA
                     SAGIIVLLVILILMNGVAIILRNKFSKKF"
     gene            3218..4069
                     /locus_tag="PPY92_001945"
     CDS             3218..4069
                     /locus_tag="PPY92_001945"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDJ2625933.1"
                     /translation="MAQTLAQTKQISQSHTFDVSQSHHKTPDDTNSHSVIYSTQNLDL
                     WYGENHALQNINLDIYENQITAIIGPSGCGKSTYIKTLNRMVELVPSVKTAGKILYRD
                     QDIFDQKYSKEQLRTNVGMVFQQPNPFPKSIYDNITYGPKIHGIKNKKVLDEIVEKSL
                     RGAAIWDELKDRLHTNAYSLSGGQQQRVCIARCLAIEPEVILMDEPTSALDPISTLRV
                     EELVQELKEKYTIIMVTHNMQQAARVSDKTAFFLNGYVNEYDDTDKIFSNPSNKKTED
                     YISGRFG"
     gene            4076..4717
                     /gene="phoU"
                     /locus_tag="PPY92_001946"
     CDS             4076..4717
                     /gene="phoU"
                     /locus_tag="PPY92_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDJ2625934.1"
                     /translation="MAIIRQRYQEQLDDLIKELRRLGANVYVSIENGIKSLSIDDRGF
                     ARQTVKNDKHINQLNYDINERVIMLITKQQPIASDLRMMISSLKIASDLERIGDNASS
                     IANIRLRTKITDDYVLTRLKTMGKLAMLMLKDLDQAFKKKDTVLIREIIERDEDIDDL
                     YSHIINATYLIDNDPFVAAQAHLAARHLERIGDHIINIAESVYFYLTGTHYEQ"
     gene            complement(4857..6671)
                     /gene="pepF"
                     /locus_tag="PPY92_001947"
     CDS             complement(4857..6671)
                     /gene="pepF"
                     /locus_tag="PPY92_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDJ2625935.1"
                     /translation="MSQGLPLREDVPVSETWDLVDLFKDDQQYYESIDALVQQANQFH
                     HTYATTLNSIEQINTALAELENILIALDRLSNYAELRLSVDTSNIEAQVLSAKLSTTY
                     GKIVSQLSFVESEILELPEEILQQLEESCPYQHYIKQLIKQKPFQLSASVEQVLATLS
                     PTLNSPYDLYGTTKMLDITFDSFEHDGTTYPVDYATFENDYEDNKDPEFRRKSFKSFS
                     DGIRKYQHTTAATYNMQVQQEKIEADLRGFESVIDYLLHSQEVTRDMFDRQIDMIMRD
                     LAPVMQKYAKLLQRIHGLDNMRFEDLKISVDPDYEPEISIEDSKNYIFGALSVLGDDY
                     TNMLREAYDQRWIDFAQNKGKDTGAFCASPYFTHSYVFISWTGKMAEAFVLAHELGHA
                     GHFTLAQKHQPYLESEASMYFVEAPSTMNEMLMANYLFNTSDNPRFKRWVIGSILSRT
                     YYHNMVTHLLEAAYQREVYHKVDQGESLNAPTLNEIMLNVYKQFFGDAVDMTEGAELT
                     WMRQPHYYMGLYSYTYSAGLTIGTVVSQKIKNEGQPAVDAWLETLKKGGSVSPVELAN
                     IAGVDITTEQPLKSTIQYISDLVDEVEKLTDEIEQANN"
     gene            6876..7220
                     /locus_tag="PPY92_001948"
     CDS             6876..7220
                     /locus_tag="PPY92_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625936.1"
                     /translation="MTLFDMPNYLWITTLIMILLTIFCCLVLNKWFVSAVITFVILGV
                     LAFFIPNFQDIKYQPLLGYAAFLAIMSLLISFLLWYFTRNWRKERKARKLEKQIEKYG
                     YEGAELRRKDKK"
     gene            7168..>7346
                     /locus_tag="PPY92_001949"
     CDS             7168..>7346
                     /locus_tag="PPY92_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625937.1"
                     /translation="MKNTAMRALNFAVKIKNNIRQLYAQNHRLLSYTAVTIISKEMEY
                     SAIVVFVLTFLYFTW"
BASE COUNT         2568 a         1128 c         1247 g         2403 t
ORIGIN      
        1 tttagattaa caaaaattaa tatttacatt ttcttaacaa atttttatgt aacatttaca
       61 gtttctaaaa atgaggttaa taattcaagg ttaagataaa gatgtaatca atacaaatac
      121 tatttgttgt tcatacaggg aggatatttc aatgaaaaaa tggcaatttg ttggtactac
      181 agctttaggt gcaacactat tattaggtgc ttgtggtggc ggtaatggtg gcagtggtaa
      241 tagtgattta aaaggggaag ctaaaggtga tggctcatca acagtagcac caattgtgga
      301 gaaattaaat gaaaaatggg ctcaagatca ctcggatgct aaaatctcag caggacaagc
      361 tggtacaggt gctggtttcc aaaaattcat tgcaggagat atcgacttcg ctgatgcttc
      421 tagaccaatt aaagatgaag agaagcaaaa attacaagat aagaatatca aatacaaaga
      481 atttaaaatt gcgcaagatg gtgtaacggt tgctgtaaat aaagaaaatg attttgtaga
      541 tgaattagac aaacagcaat taaaagcaat ttattctgga aaagctaaaa catggaaaga
      601 tgttaatagt aaatggccag ataaaaaaat aaatgctgta tcaccaaact caagtcatgg
      661 tacttatgac ttcttcgaaa atgaagtaat gaataaagaa gatattaaag cagaaaaaaa
      721 tgctgataca aatgctatcg tttcttctgt aacgaaaaac aaagagggaa tcggatactt
      781 tggatataac ttctacgtac aaaataaaga taaattaaaa gaagttaaaa tcaaagatga
      841 aaatggtaaa gcaacagagc ctacgaaaaa aacaattcaa gataactctt atgcattaag
      901 tagaccatta ttcatttatg taaatgaaaa agcattgaaa gacaataaag taatgtcaga
      961 atttatcaaa ttcgtcttag aagataaagg taaagcagct gaagaagctg gatatgtagc
     1021 agcaccagag aaaacataca aatcacaatt agatgattta aaagcattta ttgataaaaa
     1081 tcaaaaatca gacgacaaga aatctgatga taaaaagtct gaagacaaaa aataataaga
     1141 cgcaatttca aatgtgtctt gaaacatgat tttgatggtg aatcattatt tagagtacaa
     1201 agcttgattt atcgagacgc tgattttgac attcagttag tctacaagct tatcaactta
     1261 aaatagtggt tcatcattat tttacaaatc taattatttt gggagtaata gaaagaggtt
     1321 tgattatgac ttcatctact aatgttaaag ctttaatcga aaaaaatatt aataaaaaag
     1381 ggaagcataa tgacaaaatt ataccagtta ttttagccgc aatttcagcg atttccattt
     1441 taacaacact aggtatatta atcacattgc ttttagaaac catcactttt ttcaccagaa
     1501 ttccaataac tgaatttcta ttttctacta cttggaatcc taccggttca gaccctaagt
     1561 ttggtatctg ggcattgata atagggactt taaaaatcac agttattgcg actatatttg
     1621 cagttccagt cgggcttggt gcagcaattt atttaagtga atatgcaagt gaccgtgcac
     1681 gacgcataat taaaccgata ttagaaattt tagcaggaat accaacaatt gtgtttggtt
     1741 tctttgcatt aacctttgtt acaccagtat taagatcttt catatcaggt cttggagagt
     1801 ttaatgctat aagtcccggc ttagttgtcg gtattatgat tgtccctctc atcacaagtt
     1861 tgagtgagga tgcaatggca tctgtaccaa ataaaattcg agaaggtgcc tatggacttg
     1921 gagcaactaa attagaagta gcaactaaag tcgtacttcc cgcagcaaca tcaggtattg
     1981 tagcttcaat cgttctcgcg atttcaagag caattggaga aacgatgatt gtatcattag
     2041 cggcaggtag ttcgccaaca gcttcattaa gtttaacaag ttcgattcaa acaatgactg
     2101 gatatattgt tgagatagcg acaggtgatg caacatttgg atcaaatatt tattacagta
     2161 tttatgctgt agggttcaca ctatttatct ttaccttaat catgaattta ctttctcagt
     2221 ggatttctaa gcgttttagg gaggagtatt aatatggaaa cgacagataa taatagacaa
     2281 tcactcgtcg atcaacaact tgtccaaaaa catttatcat ccagaacggt taaaaataaa
     2341 gtgttcaaac tcatattttt agcatgtaca ttattaggac ttgtcgtact tattgcgttg
     2401 ttaactcaaa cattgattaa aggggtaagt catttaaatt tacagttttt cactaatttt
     2461 tcttcttcaa caccatctat ggctggcgtt aaaggcgcgt taatcggttc actttggtta
     2521 atgttaagta tcattccatt atcaatcatc ctaggaatag gtacagctat atacttagaa
     2581 gaatatgcga aaaacaacaa atttactcag tttgttaaaa tcagtatttc caatttagct
     2641 ggtgtaccat cagttgtatt tgggttatta ggttacactt tgttcgttgg tggtgcgggg
     2701 attgaagcct tgaaaatggg taacagtata ttggcagcag cgctaacaat gaccttactg
     2761 atattaccaa ttattattgt ttcaagtcag gaagcaatta gagctgtacc taactcagta
     2821 cgcgaagctt cttatggttt aggtgctaat aaatggcaaa cgataagacg tgttgtctta
     2881 ccagcagcgt tacctggtat tttaactgga ttcattttgt ctctttcaag agcactggga
     2941 gaaacagcgc cacttgtgct aatcggtata ccgactatat tattggcaac acctagaagt
     3001 atattggatc aattttcagc attaccaatc caaatattta cttgggcgaa aatgcctcaa
     3061 gaagaattcc agaatgttgc atcggcaggc attatcgttt tactagttat cttaatctta
     3121 atgaatggcg ttgcgattat tttacgtaac aaatttagta aaaaattcta atttaaacaa
     3181 tcaatctcat ttatctatta aaaagggagt tttaaatatg gcgcaaacac ttgcacaaac
     3241 taaacaaata tctcaaagtc atacgtttga tgtctcacaa agtcatcata aaacaccaga
     3301 tgatacaaac tcacattctg ttatatattc aacacaaaat ttagacttat ggtatggcga
     3361 aaatcatgca ttacaaaata ttaatttaga tatttatgaa aaccaaatta ctgccattat
     3421 aggtccatct ggttgtggca aatcaacata tattaaaact ttgaatcgaa tggttgagtt
     3481 agtaccttct gtgaaaacag ctggtaaaat attatatcga gatcaagaca tttttgatca
     3541 aaaatattct aaagaacaat tacgtacaaa tgtgggcatg gtctttcaac aacctaatcc
     3601 atttccaaaa tcaatatacg ataatattac ttacggtcca aagattcacg gtattaaaaa
     3661 taaaaaagtt cttgatgaaa tcgttgagaa atcattacgt ggcgctgcaa tttgggatga
     3721 attaaaggat aggttgcaca caaatgcata tagtttatcc ggtgggcaac aacaacgtgt
     3781 ttgtatcgcg cgttgtttag caattgaacc tgaagtcatt ttaatggatg aaccgacatc
     3841 agcattagat ccaatctcaa cattaagagt agaagagttg gttcaagaac taaaagaaaa
     3901 gtatacaatt attatggtta cacataatat gcaacaagca gctcgtgtat cagataaaac
     3961 tgcatttttc ttaaatggtt atgtcaatga atatgatgat actgataaaa ttttctctaa
     4021 cccatcaaac aagaaaacag aagattatat ttcaggaagg tttggttgat atataatggc
     4081 aataattaga cagcgatatc aggagcaact tgatgattta ataaaggaat tacgtcggtt
     4141 aggtgcaaat gtctatgtga gtattgaaaa tggtataaaa tcattaagta ttgacgatag
     4201 aggctttgca cgacaaacag ttaaaaacga taaacatatc aatcaattaa attatgatat
     4261 taatgagcga gttatcatgt taattacaaa gcaacagccc attgcgagtg atttgcgtat
     4321 gatgatttct tcattaaaaa tcgcctccga tttagaaaga ataggagata atgcatcgag
     4381 tattgccaat attcgattgc gtacaaagat tacagatgat tatgtgttaa cccgtttaaa
     4441 gacaatgggt aaattagcta tgttaatgtt aaaggactta gatcaagcat ttaaaaagaa
     4501 agataccgta ttaataagag aaataattga gcgtgatgaa gatatcgatg acttatatag
     4561 tcatattatt aacgcaacgt atcttattga taacgatcca tttgtcgctg cacaagctca
     4621 tttagcagca agacatttag aacgtattgg tgatcatatt attaacatcg ctgaaagtgt
     4681 ttatttttat ttaacaggta cacattacga acaataactt aaagttatta ctataaaatc
     4741 ccttacgata aatatatatt tctatcattc ataaaccctc aaaaaaacca agattctcac
     4801 aattagtaat gtgaaaatct tggtttatat tgttctacta taaattgtct cgcatcttag
     4861 ttatttgctt gctcaatttc atctgttaat ttttcaactt catcgactaa atcagaaata
     4921 tattgaattg tagatttaag tggctgttct gtagtaatgt ctacacctgc aatgtttgca
     4981 agttcgacag gtgatacact accacctttt ttcaatgttt ctaaccaagc atcaacagct
     5041 ggttggcctt catttttaat cttttgagaa acgacagttc cgattgttaa gccagcagaa
     5101 tacgtatacg aatataatcc catatagtaa tgaggttgac gcatccatgt taattcagca
     5161 ccctcagtca tgtctactgc atctccaaaa aattgtttat aaacatttag cattatttca
     5221 tttaatgtcg gcgcatttaa agattcacct tgatctactt tgtgatacac ttcacgttga
     5281 taagcagctt ctaataaatg ggtaaccata ttatgataat atgttctaga taaaattgag
     5341 ccaataaccc aacgcttaaa tcttggatta tcacttgtgt taaataaata attggccatc
     5401 aacatttcat tcattgtaga aggggcttca acaaagtaca ttgatgcttc tgattcaaga
     5461 tatggttgat gtttttgagc taatgtaaaa tgacctgcat gacctaattc atgtgctaag
     5521 acaaatgctt cagccatttt accagtccaa gaaataaaca catatgaatg tgtaaagtat
     5581 ggacttgcac aaaatgcgcc tgtatcttta cctttatttt gtgcaaaatc aatccatcgc
     5641 tgatcgtatg cttcacgtaa catgtttgta tagtcatcac ctaaaacact taacgcaccg
     5701 aaaatataat tttttgagtc ttcaattgaa atctctggtt cataatcagg gtctacagaa
     5761 atcttcaagt cttcaaaacg catgttatct aatccgtgaa tacgttgtaa aagtttagca
     5821 tatttctgca taactggtgc caagtcacgc ataatcatat cgatttgacg gtcaaacata
     5881 tcacgcgtta cttcttgact atgtaataaa taatcgatga ctgattcaaa tccacgtaaa
     5941 tcagcttcaa ttttttcttg ttgtacttgc atattatatg tagccgcggt agtatgctga
     6001 tattttcgaa tcccatcgct aaacgatttg aaacttttac gtctaaactc aggatcttta
     6061 ttatcttcat aatcattttc aaacgtagca tagtcgacag ggtacgttgt accatcatgt
     6121 tcaaatgaat cgaatgtaat atctagcatt tttgtcgtgc cgtataaatc gtaaggactg
     6181 tttagcgtag gtgataaagt tgctaatact tgttctaccg acgcagataa ttggaatggc
     6241 ttttgtttta ttaactgttt aatatagtgt tgatatggac atgattcttc taattgttga
     6301 agtatttctt ctggtaattc aagtatttct gactctacaa atgataattg gctaacaatt
     6361 ttaccgtatg tagtagataa tttagcgctc aatacttgtg cctcgatatt actagtatct
     6421 acacttaaac gtagttctgc ataattactt aagcgatcta aggcaattaa aatattttct
     6481 aattcagcta aagcagtatt aatttgttcg attgaattta atgttgttgc atatgtatga
     6541 tgaaattgat ttgcttgttg tactagagcg tcaatacttt cataatattg ttgatcatct
     6601 ttaaataagt ctactaaatc ccatgtttct gaaacaggaa catcttctct taaaggtaaa
     6661 ccttgagaca tataatcact cctttggtat aatacattca ttatacataa ttttgtctcc
     6721 taaacgataa tgttacgatt tccatgtcgc aaggaacaat gatttttcta atagtagata
     6781 gaatattgct tgaaactgac tgctattgtt taagttttta aaaatttata ataaaatgta
     6841 tacaaggaaa gtaatgaaat aaaaggagaa tgaatatgac attattcgat atgcctaatt
     6901 atttatggat cacaacatta ataatgattt tattaacaat attctgttgc ttagttttaa
     6961 ataaatggtt tgtatctgca gtaattacat ttgttatatt aggtgtgctt gcatttttta
     7021 ttccaaattt tcaagatata aaatatcaac cattattagg atacgctgca tttttagcga
     7081 ttatgagttt attaattagt tttctattat ggtattttac tagaaattgg cgtaaagaaa
     7141 gaaaagcgcg taaattagaa aagcaaattg aaaaatacgg ctatgagggc gctgaacttc
     7201 gccgtaaaga taaaaaataa tattcgacaa ctatatgcac aaaatcatcg acttttaagt
     7261 tacactgcag taacaattat ttcgaaagag atggaataca gtgctatagt tgtttttgtt
     7321 ttgacttttt tatattttac atggtt
//
LOCUS       DANBWH010000135         7344 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.139, whole genome shotgun sequence.
ACCESSION   DANBWH010000135 DANBWH010000000
VERSION     DANBWH010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7344
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.139"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(28..1104)
                     /locus_tag="PPY92_001950"
     CDS             complement(28..1104)
                     /locus_tag="PPY92_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDJ2625938.1"
                     /translation="MNINKKVTLQRIQTLTELHGAPGFEEEVKNYMTQQMAPYVDEFI
                     ENRMGGFFGVKKSKNPNAKRVMIAAHMDEIGFMITNITKNGMIQFTNLGGVANDIWQG
                     QRLVIKNRNGDKIIGVVSNIPKHFRTGSEGAPEIKDLTLDIGAQNEDEVRERGIDIGD
                     TIVPHTPFTQLSEHRYSAKAWDNRYGCVLAIEILELLKDIELDVDLYVGANVQEEVGL
                     RGAKASAEMIDPDVAFVVDCSPANDVKGNQPLSGELGKGTLIRIKDGTMILKPVFRDY
                     LLKLVEAHDIEHQYYMSPGGTDGGEIHKANIGIPTAVIGVCARYIHSTDSVFDIRDYF
                     AARSLLSEAICNLDNNQIETLQYK"
     gene            1191..1502
                     /locus_tag="PPY92_001951"
     CDS             1191..1502
                     /locus_tag="PPY92_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDJ2625939.1"
                     /translation="MTKLKYIIPTIIAVAIVIISTISIIQFINRKRYNPVKVLNEVKS
                     YFMNVKGSYIVYEPFVHPETDKYRLVYQGGITTIKNGQNIHYDFYADAYTGEVINIVE
                     R"
     gene            complement(1627..2469)
                     /locus_tag="PPY92_001952"
     CDS             complement(1627..2469)
                     /locus_tag="PPY92_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDJ2625940.1"
                     /translation="MKIGDISIHYLNGGNTKMDGGAMFGVVPKPLWSKQYNANERNQI
                     NLPTHPILIQTAQYNLIIDAGIGNGKLSEKQLRNFGVDEESHIIADLANYNLTPKDID
                     YVLMTHMHFDHAAGLTDQAGHAIFENAIHVVQQDEWHEFIAPNIRSKSTYWDKNKGDY
                     SNKLILFEKHFEPVPGIKMQHSGGHSFGHTIITIESQGDKAVHMGDIFPTTAHKNPLW
                     VTAYDDYPMQSIREKERMIPYFIQQQYWFLFYHDENYFAVKYSDDGENIDAYILRETL
                     VDNN"
     gene            complement(2940..3584)
                     /gene="trmB"
                     /locus_tag="PPY92_001953"
     CDS             complement(2940..3584)
                     /gene="trmB"
                     /locus_tag="PPY92_001953"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDJ2625941.1"
                     /translation="MRVRYKPWAEDYLKDHPELVDMEGQHAGEMTEWFDKTQPIHIEI
                     GSGMGQFITTLAAQNPHINYISMEREKSIVYKVLDKVKEMGLTNLKIICNDAIELNEY
                     FKDGEVSRIYLNFSDPWPKNRHAKRRLTYHTFLALYQQILNDEGDLHFKTDNRGLFAY
                     SLESMSQFGMYFTKINLNLHQEDDGSNILTEYEKKFSDKGSRIYRMEAKFHSQK"
     gene            complement(3599..4390)
                     /locus_tag="PPY92_001954"
     CDS             complement(3599..4390)
                     /locus_tag="PPY92_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HDJ2625942.1"
                     /translation="MEQFYQLGWTLDSAGGASGEAYMAEQDGQKLFLKRNSNPFIAAL
                     SAEGIVPKLVWTKRIETGEVVTAQHWKNGRELSSNEMKQTRVAHLLKKIHNSRPLLSM
                     LKRMEMEPITPEIMLNKINASLSREVLTHHIVRKSLTYLEEHIPSLDSRFFTVVHGDV
                     NHNNWLLSDRDELFLVDWEGAMIADPAIDIGMLLYNYVPQQQWSEWLETYGVQESLNL
                     NKRMKWYTVIQSIGLVQWYEEQKRYKDMNTWLKFLNEVMNSNMFI"
     gene            complement(4940..5788)
                     /gene="dat"
                     /locus_tag="PPY92_001955"
     CDS             complement(4940..5788)
                     /gene="dat"
                     /locus_tag="PPY92_001955"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HDJ2625943.1"
                     /translation="MEKIFLNGEFVSPSEAKVSYNDRGYVFGDGIYEYIRVYNGKLFT
                     VTEHYERFLRSANEIGLDLNYSVEELIELSRKLVDMNQIETGAIYIQATRGVAERNHS
                     FPTPEVEPAIVAYTKSYDRPYDHLENGVNGVTVEDIRWLRCDIKSLNLLGNVLAKEYA
                     VKYNAVEAIQHRGETVTEGSSSNAYAIKDGVIYTHPINNYILNGITRIVIKKIAEDYN
                     IPFKEETFTVDFLKNADEVIVSSTSAEVTPVIKLDGEPINDGKVGPITRQLQEGFEKY
                     IESHSI"
     gene            complement(5792..7201)
                     /gene="sapep"
                     /locus_tag="PPY92_001956"
     CDS             complement(5792..7201)
                     /gene="sapep"
                     /locus_tag="PPY92_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+)-dependent dipeptidase Sapep"
                     /protein_id="HDJ2625944.1"
                     /translation="MWKEKVQQYEDQIINDLKGLLAIESVRDDAKASEDAPVGPGPRK
                     ALDYMYEIAHRDGFTTHDVDHIAGRIEAGKGNDVLGILCHVDVVPAGDGWDSNPFEPV
                     VTEDAIIARGTLDDKGPTIAAYYAIKILEDMNVDWKKRIHMIIGTDEESDWKCTDRYF
                     KTEEMPTLGFAPDAEFPCIHGEKGITTFDLVQNKLTEDQDEPDYELITFKSGERYNMV
                     PDHAEARVLVKENMTDVIQDFEYFLEQNHLQGDSTVDSGILVLTVEGKAVHGMDPSIG
                     VNAGLYLLKFLASLNLDNNAQAFVAFSNRYLFNSDFGEKMGMKFHTDVMGDVTTNIGV
                     ITYDNENAGLFGINLRYPEGFEFEKAMDRFANEIQQYGFEVKLGKVQPPHYVDKNDPF
                     VQKLVTAYRNQTNDMTEPYTIGGGTYARNLDKGVAFGAMFSDSEDLMHQKNEYITKKQ
                     LFNATSIYLEAIYSLCVEE"
BASE COUNT         2337 a         1408 c          959 g         2640 t
ORIGIN      
        1 ttagagataa tagttgttat tacccgatta tttatattgt aatgtttcta tttgattatt
       61 atctaaatta caaatggctt ctgaaagtaa agatctagct gcaaaatagt ctcttatatc
      121 aaatactgag tctgtactat gaatatatcg tgcacataca ccaataactg cagtcggaat
      181 accaatatta gctttatgaa tttctccacc atctgttcca cctggtgaca tatagtattg
      241 atgttcaatg tcatgtgctt ctactaactt taataaataa tctctaaata caggctttaa
      301 aatcattgta ccgtctttta tgcgaattaa cgtcccttta ccaagttcac cagataatgg
      361 ttggtttcct ttaacgtcat tggcaggtga acaatcgact acaaatgcaa cgtctgggtc
      421 tatcatctct gcagatgctt tcgcacctcg taatccaacc tcttcttgaa catttgcgcc
      481 aacatacaag tctacatcta attctatatc ttttaataat tctagtattt caattgccaa
      541 gacacaacca taacgattat cccatgcttt agcactatat cgatgttcag ataactgtgt
      601 gaatggcgtg tgaggtacaa ttgtatctcc tatatctatt ccgcgctcac gcacctcatc
      661 ttcattttga gcacctatat ctaatgttaa atctttaatt tccggtgcac cttcactacc
      721 agtacgaaaa tgtttaggta tattagaaac aacaccgata attttatcgc catttctatt
      781 tttaattact aagcgttgtc cttgccaaat atcatttgca acaccaccta aatttgtgaa
      841 ttgaatcatt ccatttttag tgatatttgt aatcataaat ccgatttcat ccatatgtgc
      901 tgcaatcatt acacgttttg catttggatt tttagatttt ttcacaccaa aaaatccacc
      961 catacgattt tcaataaatt catctacata cggcgccatt tgctgagtca tataattttt
     1021 tacttcttct tcaaaacctg gtgccccatg aagctcagtt aaagtttgaa ttcgttgtaa
     1081 tgttactttt ttatttatgt tcataaaagt tctcactcct tataagttat tatatcatct
     1141 tcaatatggt aaactaatga tatgaatttg aaatcaaggg agcataaatg atgactaaac
     1201 tgaaatatat aattccaaca ataattgcag tagccattgt aattatttct accatttcaa
     1261 tcatccaatt tattaatcgt aaacgctata atcccgttaa agtacttaat gaagtaaaat
     1321 catattttat gaatgtcaaa ggctcatata tcgtttatga accattcgtt catcctgaaa
     1381 ctgataaata ccgtttagtt tatcaaggtg gaattacaac tattaaaaat ggtcaaaata
     1441 ttcattatga tttttatgca gatgcatata ctggtgaagt cattaacatt gtagagcgtt
     1501 aatacgaatt ttatccatat ttgatttgga tcgtaaaact taaaaatgag ccacacccta
     1561 attagtaaga ttggatgtgg ctcatttttt tatttttgaa aatttgttta gtaatacatc
     1621 tttattttag ttattatcaa ctaatgtttc acgtaaaata tatgcatcta tgttttcacc
     1681 atcatcgctg tattttacag caaagtagtt ttcatcatga taaaacaaga accaatattg
     1741 ttgctgaata aaatatggta tcatgcgttc tttttcacga atcgattgca taggataatc
     1801 atcatatgcc gttacccata gaggattttt atgtgcagta gttgggaata tatcacccat
     1861 atgaactgct ttatctcctt gactttcaat cgtaataatc gtgtggccaa agctatgacc
     1921 tccactatgt tgcatcttga tacccggaac cggttcaaaa tgtttttcga ataaaatcaa
     1981 cttgttacta taatcgcctt tattcttatc ccagtaagtt gatttacttc ttatattagg
     2041 tgcaataaac tcatgccact catcttgttg cacaacatga atcgcatttt caaaaattgc
     2101 atgtcccgct tgatcagtca aaccggcagc atgatcaaaa tgcatatgtg tcattagcac
     2161 ataatcaata tcctttggcg ttaaattata atttgccaaa tcagcaatta tatgactttc
     2221 ttcatctact ccaaaattac gtaattgctt ttcagataat ttaccattac caatacccgc
     2281 atctataatc aaattatatt gagccgtttg aatcaaaatt ggatgtgtcg gtaaattgat
     2341 ttgatttcgt tcatttgcat tgtattgctt tgaccacaac ggcttcggaa caacaccaaa
     2401 cattgcaccg ccatccattt ttgtattgcc accatttaga taatgaatag atatatcccc
     2461 gatcttcata acatcaccta ttctttctgt attcgttatt acaattagca tgtatttatt
     2521 acatcatatc actattcata aatgcgaaca aaaacgaaac gccccacact aatacacttc
     2581 taactactta taaagattaa tgcaatcata ttatatgtaa atgcatcata accatcggcc
     2641 tgtaatttta aattaataat taaaatttac aagtaaaatg caagctttat tcttttaaat
     2701 caaaggtatt tagtataaca ttgtttatag gcgcattatc tcctttagtt atttgtttag
     2761 tcacttttaa ttatagagat aattgcgctt tttttatatt caaaatcaaa aaatcggaca
     2821 gatgaaaagt aaaatacttt tttccatcag tccgatttat tatagaacct ttatctatag
     2881 aaccattata gtacgaaaaa acacttatga ttgtatgtgt acataccctc ataagtgttt
     2941 tatttttgtg aatggaattt cgcttccata cgataaatac gtgacccttt atccgaaaat
     3001 ttcttttcat attctgttaa aatattactg ccatcgtctt cttgatgtaa atttagattt
     3061 atttttgtaa aatacattcc aaattgagac atactttcta aactgtaggc aaatagtcct
     3121 ctgttatcag ttttaaaatg taaatctcct tcatcattta agatttgttg atacaacgct
     3181 aaaaacgtat gatacgttaa acgtcgtttt gcatgacgat tttttggcca tggatctgaa
     3241 aagttcaaat aaatacgcga aacttcgccg tctttaaaat attcatttaa ttcaatggcg
     3301 tcattacaaa taatctttaa atttgttaaa cccatctctt taactttatc caatacttta
     3361 taaacgatac ttttctcacg ttccattgaa atatagttaa tatgaggatt ttgagcagct
     3421 aatgttgtaa taaactgccc catacccgaa ccaatttcaa tgtgtatcgg ttgcgtttta
     3481 tcaaaccatt cagtcatttc ccctgcatgt tgaccttcca tgtcaaccaa ttcaggatga
     3541 tcttttaaat aatcttcagc ccatggtttg tatcgaactc tcatatttta ttctcctctt
     3601 aaataaacat gttactattc ataacttcat ttaggaattt aagccaagtg ttcatatcct
     3661 tatatctttt ttgctcttca taccattgaa caagacctat agattgaatt accgtatacc
     3721 atttcatacg tttatttaaa ttcaagctct cttgaacacc atatgtttca agccattcag
     3781 accattgttg ttgtggaaca tagttgtaaa gcagcattcc gatatcaatt gccgggtctg
     3841 caatcattgc accttcccaa tcaactaaaa atagttcatc tcgatcggat aataaccaat
     3901 tattatgatt cacatcacca tgtacaacag tgaaaaaacg cgaatctaaa ctcggtatat
     3961 gctcttctaa ataggttaat gattttctca caatatgatg tgttaaaact tctcttgata
     4021 aagaggcatt aattttatta agcataatct caggagtaat aggttccatt tccatacgct
     4081 ttaacatact taataaaggt ctagaattgt gtatcttctt taataaatgt gcaactcttg
     4141 tttgcttcat ttcgtttgaa gatagttcac gcccattttt ccaatgttgt gctgtaacaa
     4201 cctcgcctgt ttctatgcgt ttcgtccata ctaatttggg cacaatacct tctgctgata
     4261 atgccgcaat aaatggattt gaatttcgtt ttaaaaacaa cttttgtcca tcttgttcag
     4321 ccatatatgc ttcaccagat gcaccacctg ctgaatcaag tgtccaccct aattgataaa
     4381 actgctccaa ctcgtccacc tcactttcaa ttagaaaatg gctctagaaa taggtttttc
     4441 aagagccata tattctaatt tataacacca tactggtaca aatattatgt ccagataatt
     4501 attgtaaatc ctcaaccaat gcctacatta cacgactaat tttaaatcgt aatgtctgtc
     4561 attgacacca tacattctat agtcacttac ttgacatata atgttaccgt gtctaaaact
     4621 acatgttttt gaatctctgt aggcgataaa ctctagtttt caaaataatt gctatcccat
     4681 tttcatggtt agcataaatt tatgaactgt aacatttacg tacttagtaa aatatgatgc
     4741 acatcatatt tgtaactcat agaaaatttt ataattttta tcattatatt tcaactgaaa
     4801 atgagaaaca aaatggcact ttttactaat atgtgttttc taaacaacac ttttaagctt
     4861 cgttttaaat tataacataa ttcacttacg aaagttgata aatttaagta atttaatcta
     4921 aaaatatgat gaaagaattt taaatactgt gtgactctat atacttttca aatccttctt
     4981 gtagttgacg tgtaattggg ccaactttac catcattaat tggttcacca tctaatttaa
     5041 taacaggtgt aacctcagct gaagtacttg aaacaataac ttcatctgcg tttttcaaga
     5101 aatctacagt aaacgtttct tctttaaatg ggatgttata gtcttcggca atttttttaa
     5161 ttacaattcg tgtaatacca ttaagaatat agttgttaat cggatgtgta taaatcacac
     5221 cgtctttaat tgcataagca ttacttgaag atccttcagt tacagtttca cctcgatgtt
     5281 gaattgcttc aactgcatta tatttcacag catattcttt tgctaataca tttcctaata
     5341 agttcaagct tttaatgtcg caacgtaacc atcggatatc ttcaacggta acaccattca
     5401 caccattttc taaatgatca taaggacgat cataactctt tgtataagca acaattgctg
     5461 gttctacttc aggtgtcggg aagctatgat tcctttcagc tacaccacgc gttgcttgaa
     5521 tataaattgc cccagtttca atttgattca tatcaactaa tttacgagat agttcaatta
     5581 attcttctac agaataattt aaatctaaac caatctcatt ggcactacgt aaaaatcttt
     5641 cataatgttc tgttactgta aataacttac cattatatac tcgaatgtat tcataaatac
     5701 catcgccaaa tacgtatcct ctgtcgttgt atgaaacctt tgcttcactt ggacttacaa
     5761 actcaccatt taaaaaaatt ttttccatat attattcctc cacgcataat gaataaattg
     5821 cttctaagta aatactagtt gcgttaaata actgtttttt agtgatatat tcatttttct
     5881 gatgcattaa atcttcagaa tcactaaaca ttgcgccaaa tgctacaccc ttgtctaagt
     5941 ttctcgcata agtaccgcca cctatagtat aaggttcagt catatcattt gtttgatttc
     6001 tatatgcagt aactaacttt tgtacaaaag gatcattttt atcaacataa tgtggtggtt
     6061 ggactttacc taatttcact tcaaagccat attgttgaat ctcatttgca aaacgatcca
     6121 tagctttttc aaattcaaat ccttctgggt agcgtaagtt gataccgaaa agacctgcgt
     6181 tttcattatc atatgtaata acaccaatgt tagttgtcac gtcacccatg acatctgtat
     6241 ggaatttcat tcccatcttt tcaccaaaat ctgaattaaa taagtagcga ttactaaatg
     6301 ctacaaacgc ttgtgcatta ttatcaagat ttaatgatgc taagaatttt agtaagtaaa
     6361 gacccgcatt cacaccgata gatggatcca taccatgaac cgctttacct tcaactgtta
     6421 aaactagaat gccactatca acagtactat caccttgtaa atgattttgt tctaaaaagt
     6481 actcaaagtc ttgaataaca tctgtcatat tttctttaac aagcactctt gcttctgcat
     6541 gatcaggtac catgttgtaa cgttcaccag atttaaaagt tattaattca taatcaggtt
     6601 catcttgatc ttcagtaagt ttattttgaa ctaaatcaaa tgttgtaatg cctttttcac
     6661 catgaataca tggaaattct gcatctggtg caaaacctaa tgttggcatt tcttctgttt
     6721 taaaatagcg atccgtacat ttccaatcag attcttcatc cgtaccaata atcatatgaa
     6781 tacgtttctt ccaatccaca ttcatatctt ctaatatctt aattgcataa taagcagcaa
     6841 ttgttggacc tttgtcatca agtgtacctc tagctatgat agcatcttct gttacaaccg
     6901 gctcgaacgg attactatcc catccatcac cagcaggaac aacgtcaaca tgacataaga
     6961 tacctaatac gtcatttcct ttacctgcct caattcttcc tgcaatatga tccacatcat
     7021 gtgttgtaaa tccatctcta tgtgcaattt catacatgta gtctaatgcc ttacgaggac
     7081 ctggaccaac tggtgcgtct tctgatgctt ttgcatcatc tctcacactt tcaattgcta
     7141 ataatccttt taagtcatta atgatttgat cttcgtattg ttgaactttt tctttccaca
     7201 ttcgaaatcg acttcctttt ttctataagt taaattctat tttacatgaa aagatataaa
     7261 aactacaata agatgtcaga aaataataaa aaggaacaaa acgatgctat tgatatgaca
     7321 caaatcataa atagctgctt tgtt
//
LOCUS       DANBWH010000136         7313 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.140, whole genome shotgun sequence.
ACCESSION   DANBWH010000136 DANBWH010000000
VERSION     DANBWH010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7313
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.140"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(5..367)
                     /locus_tag="PPY92_001957"
     CDS             complement(5..367)
                     /locus_tag="PPY92_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDJ2625945.1"
                     /translation="MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVA
                     AITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIRTLDKKRLKEKLTYLSDDKMKEV
                     DNALMISLGLNAVAHQKN"
     gene            complement(364..534)
                     /gene="mazE"
                     /locus_tag="PPY92_001958"
     CDS             complement(364..534)
                     /gene="mazE"
                     /locus_tag="PPY92_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HDJ2625946.1"
                     /translation="MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDC
                     NETYLSSNSTNE"
     gene            complement(619..1767)
                     /gene="alr"
                     /locus_tag="PPY92_001959"
     CDS             complement(619..1767)
                     /gene="alr"
                     /locus_tag="PPY92_001959"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDJ2625947.1"
                     /translation="MSDKYYRSAYMNVDLNAVASNFKVFSTLHPNKTVMAVVKANAYG
                     LGSVKVARHLMENGATFFAVATLDEAIELRMHGITAKILVLGVLPAKDIDKAIQHRVA
                     LTVPSKQWLKEAIKNISGEQEKKLWLHIKLDTGMGRLGIKDTNTYQEVIEIIQQYEQL
                     VFEGVFTHFACADEPGDMTTEQYQRFKDMVNEAIKPEYIHCQNSAGSLLMDCQFCNAI
                     RPGISLYGYYPSEYVQQKVKVHLKPSVQLIANVVQTKTLQAGESVSYGATYTATDPTT
                     IALLPIGYADGYLRIMQGSFVNVNGHQCEVIGRVCMDQTIVKVPDQVKAGDSVILIDN
                     HRESPQSVEVVAEKQHTINYEVLCNLSRRLPRIYHDGDQRFVTNELLK"
     gene            complement(1833..2192)
                     /locus_tag="PPY92_001960"
     CDS             complement(1833..2192)
                     /locus_tag="PPY92_001960"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDJ2625948.1"
                     /translation="MIHGIGVDLIEIDRIKVLYSKQPKLVERILTKNEQHKFNNFTHE
                     QRKIEFLAGRFATKEAFSKALGTGLGKHVAFNDIDCYNDELGKPKIDYEGFIVHVSIS
                     HTEHYAMSQVVLEKSAF"
     gene            complement(2196..2687)
                     /locus_tag="PPY92_001961"
     CDS             complement(2196..2687)
                     /locus_tag="PPY92_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HDJ2625949.1"
                     /translation="MRDYNFMSPHAKKVMRLSAVLFWIPIALVLLIAFNILNWLFWEI
                     LDNNISIVSSVIIILLVAVFTLVIAPVYRYKHCRYAFEKHHLRVRNGILFLDEKVIPY
                     FRIQNIDIDVGPIMRRYRLATLTLYTAGGQAEIELIDRSEARRLKRWLNNERHLENNK
                     NEE"
     gene            complement(2674..4257)
                     /locus_tag="PPY92_001962"
     CDS             complement(2674..4257)
                     /locus_tag="PPY92_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HDJ2625950.1"
                     /translation="MSEPQKLHPISYFNGIINAIKQNIVVFFIFIIFQLKDFDYTNPE
                     SYLWIGIVFVFFLISYISQIVGIMNTRYWIEDNYFILTTGIFNKKRKELNIKRIQSVD
                     MTQGVVNQIIGGVDLQIKTPSDGIVLSVISKKQGEYLERYIDQLQTELKTEVAQKSSA
                     EKNDNCNHDIEGNVEDIAEGRLDEVQNTDSMLNQKNKRIPIYQMNFKESLFMAMTSGA
                     IGVTLAALVPIYGAVRELIPWSKLNHEISQWVQIVSISVLIIILLVLIVAYIIGTIIT
                     MIRFYGFSVMLENEQLKIEYGLFNKKKVTVPTKRLQAVVERQSYIRRLFGYTAIHFII
                     TSDFENKKIEDDDNDSGSVPILPFIKRDKAYQIIQNLVPDLSYQQVNEGMPLSGFHRH
                     FLIPSIIVIAMAGIGCYFWNQLLLLFTIITIVIIGLFVIKGYLYVKHAGFKMTEEQIT
                     IQNFELLKRNIYYFKAHHIIGFDTWQHTLLKRNNLNNFSFIVAKGVGAQSINLKYARS
                     EDANKLKSWYLRGENDEGL"
     gene            complement(4250..4729)
                     /locus_tag="PPY92_001963"
     CDS             complement(4250..4729)
                     /locus_tag="PPY92_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625951.1"
                     /translation="MSDYTKLTKSPKSALLYYYITNGLEFILSVAVYVIFYFIWLRFE
                     WPHYLLYILLVLGALSTLKLFIKPLWQYHCRFYKVDQLSVQYRTSFFIYKEETSRIER
                     LQYLSIKSNPISKVLNLYKVGFMTAGHTIYLPMMSHDDVKIIEARTMSNLRGVESDV"
     gene            complement(4937..5497)
                     /locus_tag="PPY92_001964"
     CDS             complement(4937..5497)
                     /locus_tag="PPY92_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDJ2625952.1"
                     /translation="MNTIRNSICLTIITMVLCGFLFPLAITLIGQIFFYQQANGSLIT
                     YDNRIVGSKLIGQHWTETRYFHGRPSAVDYNMNPEKLYKNGVSSGGSNESNGNTELIA
                     RMKHHVKFGNSNVTIDAATSSGSGLDPHITVENALKQAPRIADARHVSTSRVADLIQH
                     RKQRGVLTNDYVNVLELNIALDKMKD"
     gene            complement(5517..>7313)
                     /gene="kdpB"
                     /locus_tag="PPY92_001965"
     CDS             complement(5517..>7313)
                     /gene="kdpB"
                     /locus_tag="PPY92_001965"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDJ2625953.1"
                     /translation="FANFSEAFAEGRGKAQADSLRQAQSNLTARLIEENGAYRIVNAT
                     ELKAGQNIRVENGETIPADGVVINGLATVDESAITGESAPVIKESGGDFDGVIGGTLV
                     TSDWLEIRVESEAGTSFLDKMIALVEGAERNKTPNEIALFTLLTTLTIIFLVVIVTLY
                     PIASYLHLILPIAMLIALTVCLIPTTIGGLLSAIGIAGMDRVTQFNVLAKSGRAVEVC
                     GDVDVMILDKTGTITYGNRIASEFLPVNQQMLEKLIVAAYMSSIYDDTPEGKSIVRLA
                     KQMYINELPKDIDGTYKPFTAETRMSGIITNEISVFKGAPNSMINLVKQQQGNIPLNI
                     ESLCMDVSSKGGTPLIVIENNVMLGVIYLKDVIKDGLVERFTELRKMGIETVMCTGDN
                     ALTAATIAKEARVDRFVAECKPEDKIKVIKDEQAKGHIVAMTGDGTNDAPALAQANIG
                     LAMNSGTISAKEAANLIDLDSNPTKLIEVVKIGKQLLMTRGALTTFSLANDVAKYFAI
                     LPALMMSTIPEMTSLNIMHLSSPKSAIISALIFNALIIVALIPIAMKGVKVKGYSIDR
                     IFINNMLIYGLGGLIVPFLGIKLIDMIVQFFV"
BASE COUNT         2282 a         1454 c         1024 g         2553 t
ORIGIN      
        1 acgcctaatt tttctggtga gctactgcat tcagccctaa actaatcatt agtgcattat
       61 ctacttcttt cattttatca tcggataagt acgtcagttt ttctttcaat cgttttttat
      121 caagtgtacg aatttgttct aataatataa ctgagtcttt atccaattta tactttttct
      181 tttcaatctc tacatgtgtc ggtattttcg ctttattaat cctaccagtt attgccgcaa
      241 caataactgt aggactatat ttattaccag tatcattttg aattatgact acaggtctga
      301 ctcccccttg ttcagatccc tgtactggtg ataaatctgc taaataaaca tctcctcgtc
      361 taatcattca ttcgttgaat tagaagataa atatgtttcg ttgcaatcgc atgcttcaca
      421 ctctatcgga aaagcttcgt tcgctaggga gagatttaaa tcagccattt gtgaatatcc
      481 ttcttttaaa gattgttcta agctatgact tctattttga ctaaaagata acatatgaga
      541 acctccaatt gattccaaaa gttattaaat gacctttaat tactctaatg ataacaaatt
      601 ttatatgcac tgaccatatt attttaacaa ttcatttgtt acaaaacgtt gatcaccatc
      661 atgatagatt cgcggcaaac gtctcgacaa gttacaaagc acttcataat taatagtatg
      721 ttgcttttca gctaccacct ctaccgactg tggactttct ctatgattat ctattaaaat
      781 caccgaatct ccagctttaa cttgatctgg cactttaaca attgtctgat ccatacatac
      841 gcgaccaata acttcgcatt gatgaccatt tacatttaca aagctacctt gcattatgcg
      901 taaatagcca tctgcatatc caataggtaa caaagctatt gtagttgggt cagtagctgt
      961 ataagttgca ccataactta cagactcacc cgcttgtagc gtctttgttt gaactacatt
     1021 agcaattaat tgcacacttg gtttaaggtg tactttaact ttttgctgta catactctga
     1081 tggataatat ccataaaggg aaattcctgg tcttattgca ttacagaatt ggcaatccat
     1141 taatagagag cctgctgagt tctgacaatg tatatattca ggtttaattg cttcattgac
     1201 catatcttta aaacgttgat attgttcagt tgtcatatct cctggttcgt cagcacaggc
     1261 aaagtgtgta aacacgcctt caaatacaag ttgctcatat tgttgaatga tttcaatcac
     1321 ttcttgatac gtattagtat ctttaatacc taaacgtccc attcctgtat ctaatttaat
     1381 gtgcaaccat aactttttct cttgctcacc agaaatgttt ttaattgctt ctttcaacca
     1441 ctgtttagac ggaaccgtta aggcaactct gtgttgtatc gctttatcaa tatctttagc
     1501 tggtaacaca cctaagacta aaattttagc agtaatccca tgcattctaa gttctatcgc
     1561 ttcatctaac gttgctacag caaaaaatgt ggcgccattt tccattaaat gacgtgctac
     1621 tttaacacta cctagtccat aggcattggc tttaacgaca gccatcactg ttttatttgg
     1681 atgcaatgta ctgaatactt tgaaatttga tgcaacagcg tttaaatcta cattcatata
     1741 cgcagatcta taatatttat ccgacatatt acttcctcct gtaattccca cacgttttaa
     1801 aactagatct taattatcat tgtataacaa atttaaaatg ctgacttttc taaaacaact
     1861 tggctcatcg cataatgctc agtgtgtgag atactaacat gtacgatgaa cccttcgtaa
     1921 tcaatctttg gtttgccaag ttcgtcgttg taacagtcta tatcgttaaa agctacatgt
     1981 tttcctaagc ctgtgcctaa tgctttactg aacgcttctt ttgtagcaaa cctgccagct
     2041 aaaaattcaa ttttacgttg ctcatgtgtg aaattgttga atttgtgctg ttcattttta
     2101 gttaaaatcc gctcaaccaa ttttggctgc ttactatata acacttttat tcgatcgatt
     2161 tcgattaaat ctacaccaat tccatgtatc attgattatt cctcattttt attgttttct
     2221 aaatgtctct cgttatttaa ccatcgtttc aatctacgtg cttcacttct atctattaat
     2281 tcaatttcag cttgaccacc cgctgtataa agggtcaaag ttgctaatcg atagcgtctc
     2341 attataggac caacatcgat atcaatattt tgaatacgaa aatatggtat tactttttca
     2401 tccaaaaata aaatgccgtt tcgtacacgc aaatggtgtt tttcaaatgc atatctgcag
     2461 tgcttatatc gatatactgg cgctatgaca agcgtgaaaa cagcaacaag taatattata
     2521 atcacactac taacaatgga tatattgtta tctaatatct cccaaaatag ccagttcaaa
     2581 atattaaatg cgattaaaag tacaagcgct atgggtatcc aaaacagcac agcacttaac
     2641 ctcatcactt ttttagcgtg tggtgacata aaattataat ccctcatcat tttcacctct
     2701 taaataccat gatttcaatt tatttgcatc ttcacttctt gcgtatttta ggttaatcga
     2761 ttgggctccc acacctttag caacaataaa gctaaaatta tttaaattgt tgcgtttaag
     2821 taatgtatgt tgccaagtgt caaatcctat aatgtgatgc gctttaaaat aataaatatt
     2881 tcgtttcaat agctcgaaat tctggatagt aatttgttct tctgtcattt taaaacccgc
     2941 atgtttgaca taaagatatc ctttgatcac aaataaacca ataatgacta ttgttataat
     3001 cgtaaataac aataataatt gattccaaaa ataacagcca atacctgcca tagctatgac
     3061 aataatacta ggtattaaaa agtgtctgtg gaaacctgac aaaggcatac cttcattaac
     3121 ttgttgataa gataaatctg gtactaaatt ctggataatt tgatatgctt tgtctcgttt
     3181 aataaacggc aatatcggca cactacctga atcattgtca tcatcttcga tttttttatt
     3241 ttcaaaatca cttgtgatga taaaatgtat tgctgtgtag ccaaataatc ttcgtatata
     3301 tgactgacgt tcaacaacag cttgcaatcg tttcgttggt acagttactt tcttcttgtt
     3361 aaagagacca tattcaattt taagttgctc attttcaagc atcaccgaaa agccataaaa
     3421 tcttatcatt gttataatcg ttccaataat atatgccact attaatacta gtaaaatgat
     3481 gattaatact gaaatactta caatttgaac ccattgacta atttcatgat ttagcttcga
     3541 ccatgggatc aactctctta cagccccgta aatcggtact aaagctgcta acgttacacc
     3601 aatggcgcca ctggtcattg ccataaatag tgattcttta aaattcatct gatatatagg
     3661 aatgcgttta tttttctgat taagcatact atcagtgttc tgcacttcat ctaagcgacc
     3721 ttctgcgatg tcttccacat taccttcaat gtcatgatta cagttgtcat tcttctcagc
     3781 actagacttt tgcgccactt ctgtcttcaa ctctgtttgc aattgatcaa tatatcgttc
     3841 aagatattca ccttgttttt tcgaaataac acttaagaca ataccatcac ttggtgtttt
     3901 gatttgtaaa tccacaccac caataatttg attgacgacc ccttgtgtca tatcaacgga
     3961 ctgaatacgt tttatattta attctttacg ctttttatta aaaatacctg ttgttaaaat
     4021 gaaataatta tcctcaatcc aatatcgcgt gttcataatt ccgacaattt gagaaatgta
     4081 tgatattaaa aagaatacaa atacaatacc tatccataaa tatgattcgg gattcgtata
     4141 atcaaaatct ttcaattgaa agataatgaa aataaaaaag acgactatgt tttgtttgat
     4201 agcattgatt atgccattaa aatatgaaat cggatgtaat ttttgaggtt cagacatcac
     4261 tttcaacccc tctcaaattc gacatagttc tcgcttcgat tattttaaca tcgtcatgag
     4321 acatcatcgg taaataaata gtatgacctg cagtcataaa tccaacttta tacaaattaa
     4381 gcactttact aattggatta gatttaatcg acaagtattg taaacgttca attcgactcg
     4441 tttcttcttt atatataaaa aatgatgtac gatattgtac acttagttga tcaactttat
     4501 aaaagcgaca atgatattgc cataaaggct taataaataa ttttaatgta ctcagagcac
     4561 ctaaaaccaa caaaatataa agtaagtaat gtggccattc aaatcttaac catataaaat
     4621 aaaaaatgac atacacagct acactcaata taaattctaa gccattcgta atgtagtaat
     4681 acaacaatgc tgacttagga ctcttagtca acttagtata atctgacata acccctctcc
     4741 ccaaataaaa aattatacgg atttataatc tatttcattt tatttttata tgatgataat
     4801 tatagcatat ggaatatttc atgctaattt attcttccta aaggtacatc taaaaattta
     4861 attaagcaga aagtgcttga attgctaaaa agacaccatg ttataatttt atcaacatga
     4921 tgcctttcat ctataatcaa tctttcatct tatcaagagc gatatttagt tcaagcacat
     4981 tcacataatc atttgttaac acaccacgct gcttacgatg ttgaatcaag tcggccactc
     5041 ttgaagtaga tacatgacga gcatcagcaa tacgaggtgc ttgcttcaat gcattttcga
     5101 ccgtaatatg cggatctaag cccgacccag aacttgttgc agcatctatt gttacatttg
     5161 aattcccaaa tttaacatga tgtttcatgc gtgctattaa ttcggtgttt ccattcgatt
     5221 cattacttcc acctgaagat acgccgtttt tatataattt ttcaggattc atattataat
     5281 caactgcact cggtctcccg tgaaaatatc gtgtctctgt ccagtgctgt ccaatcaatt
     5341 ttgatccaac tatacgattg tcatacgtaa ttaaactgcc atttgcttgt tgataaaaaa
     5401 atatttgccc aattaacgtg atagctaacg ggaataaaaa tccacataat accatagtta
     5461 ttatcgttaa acaaatacta tttcttatcg tattcatggt acaggctcct tcctctttac
     5521 acaaaaaatt gtacaatcat atctattaat ttaatgccta aaaacgggac gattaatcca
     5581 cctaatccat aaatcaacat attatttata aagattctat caatgctgta accctttact
     5641 tttacacctt tcatggcaat tggaattaag gcaacaatga ttaatgcatt gaatatcaaa
     5701 gctgatataa ttgcagattt aggtgacgat aaatgcataa tatttagtga agtcatttct
     5761 ggaattgttg acatcattag tgcaggtaaa attgcaaagt attttgctac gtcattagcc
     5821 aaactaaatg tcgttaatgc acctctcgtc attaataatt gtttgcctat ttttacaacc
     5881 tctattaact ttgtaggatt cgaatctaaa tcaattagat tagctgcctc tttagcacta
     5941 attgtccctg agttcatagc taatcctata ttcgcttgtg ctagcgcagg tgcatcattt
     6001 gtaccatctc ctgtcatcgc aacaatatgg cctttcgctt gttcatcttt gatgacttta
     6061 attttatctt cgggtttaca ctctgcaaca aatctatcaa ccctggcttc ttttgcaatt
     6121 gtagctgctg ttaaagcatt atcacctgta cacataactg tttcaatccc catttttctc
     6181 aattcagtaa atcgttctac aagaccatct ttaatcacat cttttaaata aatcacgcca
     6241 agcatgacat tgttttcaat gactattaat ggtgtgccac ctttactcga tacatccata
     6301 cagagagact caatgttaag aggaatattg ccttgttgtt gtttgacaag atttatcata
     6361 ctattaggtg cacctttgaa taccgatatt tcatttgtaa tgattccgct cattctagtt
     6421 tcagctgtaa aaggcttata tgtgccatca atgtctttag gcagctcatt tatatacatc
     6481 tgcttcgcta atcgtacaat actttttcct tctggcgtat catcgtagat tgatgacata
     6541 taagcagcga ctatcaattt ttccagcatt tgttgattca ctggtaaaaa ttcactagcg
     6601 attcgattgc cataagtgat tgtccctgtc ttgtctaaaa tcattacatc gacatctcca
     6661 catacttcta cagcacgccc acttttcgct aatacattga attgagtaac acgatccatg
     6721 cctgcaatac caatcgccga taacaaacca ccgattgtcg ttggtattaa acatactgtt
     6781 aacgcaatga gcatcgcaat aggtaaaatt aaatgcaggt aagatgctat tggatataac
     6841 gttacaataa cgactaaaaa tataattgtt aacgttgtta ataatgtaaa aagtgcaatt
     6901 tcatttggtg ttttatttct ttccgcccct tcaactaagg caatcatttt atctaaaaaa
     6961 gatgtacccg cttcactctc aacacgtatt tctaaccaat cagatgttac aagtgtaccg
     7021 ccaatgactc catcaaaatc gccacctgat tcttttatca caggtgcaga ctcaccagta
     7081 attgcagatt catcaacggt tgctaatcca tttattacaa cgccatcagc agggattgtt
     7141 tctccatttt ctacccgaat attttgtccg gcttttaact ctgtggcgtt cactatccga
     7201 tacgcaccat tttcttctat caatcgagca gttaaatttg attgtgcttg tcttaaacta
     7261 tcagcttgcg cttttccacg accttcagca aaggcttctg aaaaattagc aaa
//
LOCUS       DANBWH010000137         7307 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.141, whole genome shotgun sequence.
ACCESSION   DANBWH010000137 DANBWH010000000
VERSION     DANBWH010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7307
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.141"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..266)
                     /locus_tag="PPY92_001966"
     CDS             complement(<1..266)
                     /locus_tag="PPY92_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3055 domain-containing protein"
                     /protein_id="HDJ2625954.1"
                     /translation="MIDMYLYDDNEESQVQFVGFVGEHSRYDLMLVHTNRHYGKTLVL
                     NMQTNKFGIIGTDDLKEEGYIAHILGVNAEEGDEITEYLNEVIH"
     gene            364..798
                     /locus_tag="PPY92_001967"
     CDS             364..798
                     /locus_tag="PPY92_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HDJ2625955.1"
                     /translation="MYFVDKDKLTQKLAYLQALTDDYHESKHNHYAFERIAQMLIESS
                     VDIGNMIIDAFILRDPGNYKDVIDILELENVITKETQQEINKTVDIRKQFTYDYTALD
                     IKVIMPMFDDALPYYKQFITEVTIFLHQENVPVTAFGKGENQ"
     gene            798..1577
                     /locus_tag="PPY92_001968"
     CDS             798..1577
                     /locus_tag="PPY92_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HDJ2625956.1"
                     /translation="MKQYKAYLIDLDGTMYMGTDEIDGAKQFIDYLNVKGIPHLYVTN
                     NSTKTPEQVTEKLREMHIDAKPEEVVTSALATADYISEQSPGASVYMLGGSGLNTALT
                     EAGLVIKNDEHVDYVVIGLDEQVTYEKLAIATLGVRNGATFISTNPDVSIPKERGLLP
                     GNGAITSVVSVSTGVSPQFIGKPEPIIMVKALEILGLDKSEVAMVGDLYDTDIMSGIN
                     VGMDTIHVQTGVSTLEDVQNKNVPPTYSFKDLNEAIAELEK"
     gene            1610..2569
                     /locus_tag="PPY92_001969"
     CDS             1610..2569
                     /locus_tag="PPY92_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HDJ2625957.1"
                     /translation="MVKIVVSRKIPDKFYQQLSKLGDVVMWQKSLVPMPKDQFVTALR
                     DADACFITLSEQIDAEILAQSPNLKVIANMAVGYDNIDVESATANNVVVTNTPNVLTE
                     TTAELGFTLMLAIARRIVEAEKYVEADAWQSWGPYLLSGKDVFNSTIGIYGMGDIGKA
                     FARRLQGFNTNILYHNRSRHKDAEADFNATYVSFETLLAESDFIICTAPLTKETHHKF
                     NAEAFEQMKNDAIFINIGRGQIVDETALIDALDNKEILACGLDVLANEPIDHTHPLMG
                     RDNVLITPHIGSASVTTRDNMIQLCINNIEAVMTNQVPHTPVN"
     gene            2823..2933
                     /locus_tag="PPY92_001970"
     CDS             2823..2933
                     /locus_tag="PPY92_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625958.1"
                     /translation="MMTTNYYVESIKLKLNFIMNIDIMNCKKQILKRILY"
     gene            2981..3133
                     /locus_tag="PPY92_001971"
     CDS             2981..3133
                     /locus_tag="PPY92_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HDJ2625959.1"
                     /translation="MKSKSKQPPNKYVEAFKPYLLTLLYLAIFITLYLIYGSGDTHNN
                     FIYNEF"
     gene            3149..4606
                     /gene="dltA"
                     /locus_tag="PPY92_001972"
     CDS             3149..4606
                     /gene="dltA"
                     /locus_tag="PPY92_001972"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HDJ2625960.1"
                     /translation="MTDIINKLQAFADANPQSIAVRHTTDELTYQQLMDESSKLAHRL
                     QGSKKPMILFGHMSPYMIVGMIGAIKAGCGYVPVDTSIPEDRIKMIINKVQPEFVFNT
                     TDESFESLEGEVFTIEDIKTSQDPVIFDSQIKDNDTVYTIFTSGSTGEPKGVQIEYAS
                     LVQFTEWMLELNKSGNKQQWLNQAPFSFDLSVMAIYPCLASGGTLNLVDKNMINKPKL
                     LNEMLTATPINIWVSTPSFMEMCLLLPTLNEEQYGSLNEFFFCGEILPHRAAKALVSR
                     FPSATIYNTYGPTEATVAVTSIQITQEILDQYPTLPVGVERLGARLSTTDDGELVIEG
                     QSVSLGYLKNDQKTAEVFNFDDGIRTYHTGDKAKFENGQWFIQGRIDFQIKLNGYRME
                     LEEIETQLRQSEFVKEAIVVPVYKNDKVIHLIGAIVPTTEVTDNAEMTKNIKNDLKSR
                     LPEYMIPRKFEWMEQLPLTSNGKIDRKKIAEVING"
     gene            4603..5817
                     /gene="dltB"
                     /locus_tag="PPY92_001973"
     CDS             4603..5817
                     /gene="dltB"
                     /locus_tag="PPY92_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PG:teichoic acid D-alanyltransferase DltB"
                     /protein_id="HDJ2625961.1"
                     /translation="MIPYGDFTFFLIALIALLPVIILGFLGKRSYIYNGVVTAFMIVL
                     IFSSDKHNLFDQKYLSVQLISFIIYVVWQVLLIMFYYHSKPKNNSFSKFVTVMVLSIL
                     PLALVKVLQSTWLGGHQIHFHESKLIEFVGFLGISYVTFKSVQLIMEIRDGSIKEIKV
                     WKLIQFISFFPTISSGPIDRYKRFVKDDKKVPTGNEYRELVLKAIHMIMLGFLYKYIV
                     AYFINTYAIMPLQLDLHGFVNLWLYMYAYSLYLFFDFAGYSLFAIAFSYLFGIKTPPN
                     FDKPFKAKNIKDFWNRWHMTLSFWFRDCIYMRSLFYMSRKKLLKSQFAMSNVAFLINF
                     FIMGIWHGIEVYYIVYGLYHAALFIGYGYYERWRKKHPPRWQNGFTTALSIVITFHFV
                     TFGFLIFSGKLI"
     gene            5835..6071
                     /gene="dltC"
                     /locus_tag="PPY92_001974"
     CDS             5835..6071
                     /gene="dltC"
                     /locus_tag="PPY92_001974"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     2"
                     /protein_id="HDJ2625962.1"
                     /translation="MEFREQVLNLLAEVAENDIVKENPDVEIFEEGIIDSFQTVGLLL
                     EIQNKLDIEVSIMDFDRDEWATPNKIVEALEELR"
     gene            6068..7243
                     /gene="dltD"
                     /locus_tag="PPY92_001975"
     CDS             6068..7243
                     /gene="dltD"
                     /locus_tag="PPY92_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDJ2625963.1"
                     /translation="MKLKPFLPILISGAVFIVFLLLPASWFTGLVNEKTVEDNRTSLT
                     DQVLKGTLIQDKLYESNKYYPIYGSSELGKDDPFNPAIALNKHNANKKAFLLGAGGST
                     DLINAVELASQYDKLKGKKLTFIISPQWFTNHGLTNQNFDARMSQTQINQMFQQKNMS
                     TELKRRYAQRLLQFPHVHNKEYLKSYAKNPKETKDSYISGFKENQLIKIEAIKSLFAM
                     DKSPLEHVKPATKPDASWDEMKQKAVEIGKADTTSNKFGIRDQYWILIQESKRKVRRD
                     YEFNVNSPEFQDLELLVKTMRAAGADVQYVSIPSNGVWYDHIGIDKERRQAVYKKIHS
                     TVVDNGGKIYDMTDKDYEKYVISDAVHIGWKGWVYMDEQIAKHMKGEPQPEVDKPKN"
BASE COUNT         2639 a         1038 c         1283 g         2347 t
ORIGIN      
        1 taatgaatga cttcatttaa atactcagta atttcatcgc cttcttcagc atttacacct
       61 aaaatatgag cgatatagcc ttcttctttt aaatcatcag taccgataat accgaattta
      121 tttgtttgca tattaagtac gagtgtctta ccataatgtc tatttgtatg gactaacatc
      181 aaatcatatc gactatgctc gccaacaaaa ccaacaaact gaacttgact ctcttcgttg
      241 tcatcatata aatacatatc aatcattttg tagcgactcc ttttaaaagt agtaaagtta
      301 gtataacgac aaatgaagta tactgcaaaa ttatgataat atataagcga gaggtgacaa
      361 ggaatgtatt ttgtagacaa agataaacta actcagaaat tagcctattt acaagcatta
      421 actgatgatt atcatgagag caagcacaat cattatgcat ttgaacgcat tgctcaaatg
      481 ttgatagaat catcggtaga tatagggaat atgattatcg atgcatttat tttaagggat
      541 cctggtaatt ataaagatgt gattgatata ttagaactag aaaatgttat tactaaagaa
      601 acacagcagg agattaataa aactgtcgat attcgtaaac aatttacata tgattacaca
      661 gccttagata ttaaggttat catgccaatg tttgatgacg cattacctta ttacaaacaa
      721 tttattacag aagtaacgat atttttacat caagaaaatg taccagtaac agcttttggt
      781 aaaggagaaa atcaataatg aaacagtata aagcgtattt aatcgattta gatggcacaa
      841 tgtatatggg aacagatgag attgatggag caaaacaatt catcgattat ttaaatgtaa
      901 aaggcattcc tcatttatac gtaactaata attcaacaaa aacacctgag caagtaactg
      961 aaaaattacg tgaaatgcac attgatgcta aaccagaaga ggttgtaacg tcagcgttag
     1021 ccactgctga ttatatttca gaacaatcac caggagcatc agtatatatg ttaggtggga
     1081 gtggtttaaa tactgcgtta accgaagcgg gacttgtcat taaaaatgac gagcatgttg
     1141 attatgtagt tattggactt gacgaacaag ttacatatga aaagcttgcg attgcaacgt
     1201 taggtgtaag aaatggtgca acatttattt ctacaaatcc tgatgtatca attcctaaag
     1261 agcgtggttt attacctggt aatggtgcta ttacaagtgt tgtaagtgta tcgacaggtg
     1321 tatcgccaca atttattggt aaaccagaac cgattattat ggttaaagca ttagaaattt
     1381 taggattaga taaatccgaa gttgctatgg taggcgattt gtacgatacc gatattatgt
     1441 ctggtattaa cgtaggtatg gatacgattc atgtacaaac aggtgtatct acgttagaag
     1501 atgtgcaaaa taaaaatgtg ccaccaacgt attcttttaa agatttaaat gaagcaatag
     1561 ctgaattaga aaaatagata tagtcatttt ataaagtagg tgaattgatt tggtaaaaat
     1621 agttgtttcg aggaaaattc cagataaatt ttatcaacaa ttaagtaaac ttggtgacgt
     1681 tgttatgtgg caaaaatcat tagtgcctat gcctaaagat caatttgtga cagcacttcg
     1741 tgacgcagat gcttgtttta ttacattaag tgaacagatc gatgcagaaa ttttagcaca
     1801 atcaccaaat ttaaaagtaa ttgcgaatat ggctgtagga tatgacaaca tcgatgttga
     1861 aagtgcaaca gcgaataacg tggttgtcac gaatacacca aatgtactta ctgaaacaac
     1921 tgcagaatta ggatttacat taatgcttgc tatagcacgc cgtattgtag aagctgaaaa
     1981 atatgtagaa gcagatgcat ggcaaagctg gggtccttat ttattgtcag gtaaagatgt
     2041 cttcaattca actattggaa tatatggtat gggagatatt ggtaaagctt ttgcaagaag
     2101 gttgcaaggg tttaatacta atattcttta tcataatcga tcaagacata aagatgcaga
     2161 ggcggacttt aatgcaacat atgtttcttt tgaaacgttg ttagcagaaa gtgattttat
     2221 catctgtaca gcgccactta caaaagaaac acatcataaa tttaatgctg aagcatttga
     2281 acaaatgaaa aatgatgcaa tttttattaa tatcggtaga gggcaaattg tagatgaaac
     2341 agcattaatc gatgcactag acaataaaga aattttagca tgtggtttag atgtattagc
     2401 aaatgaaccg attgatcata cacatccatt aatgggacgt gataatgttc tgattacacc
     2461 acacattggt agcgcatcag taacaacacg ggacaatatg attcaattat gtattaataa
     2521 tatagaagcg gttatgacaa atcaggtacc acatactcca gtaaattgaa aataagtatg
     2581 gtttttaatc attggtaaaa caagcaaagc aattgttaaa tatgttatgc ttgacttgcg
     2641 aatgtatata tttgcgctga tgataattca ataacattat aaactcgtca tattaatgtg
     2701 atgagtttat ttgattttga cgtatataac aaaattgtta tatagactgt aattgtttat
     2761 tgtttaaata acctttaaaa tgaatgatgt tctcaacaaa gtgtatcatt tacaataatt
     2821 gagtgatgac aaccaattac tatgttgaat ctataaaatt aaaattgaat ttcattatga
     2881 atatcgatat aatgaattgt aaaaaacaaa ttttaaaacg tattttatat tgaacggtct
     2941 ttataaaggc gtttttacta agaactttaa gaggtgcaat atgaaatcta aaagtaaaca
     3001 gccacctaat aaatatgttg aagcattcaa accatattta ttaacactat tgtatttggc
     3061 aatatttatt actttatatt taatttatgg cagtggcgac acacacaata acttcattta
     3121 taatgagttc taatgaggga gacttaatat gacagatatt attaacaagc tgcaagcgtt
     3181 tgcggatgca aatccacaaa gcattgctgt tagacacaca actgatgaat taacttatca
     3241 acagttaatg gatgagtcta gtaaattagc acatcgatta caaggtagta agaaaccgat
     3301 gattttattc ggtcacatgt caccatatat gattgttggg atgattggtg ccattaaagc
     3361 aggatgtgga tatgtacctg tagacacttc aattcctgaa gaccgtatta aaatgattat
     3421 taacaaggtt caaccagagt ttgtatttaa tacgactgat gaatcatttg aaagtttaga
     3481 aggcgaagta tttacaattg aagatattaa aacatctcaa gacccagtaa tttttgatag
     3541 tcagattaaa gataacgaca cagtatacac aatctttaca tctggttcta ctggggaacc
     3601 taaaggtgtt caaattgaat atgcaagttt agttcaattt actgagtgga tgttagaact
     3661 taataaatca ggaaataaac aacaatggct taaccaagcg ccattttcat ttgatttatc
     3721 tgtaatggct atttatccat gtttagcatc aggcggtaca ttaaatcttg tagataaaaa
     3781 catgattaat aaacctaaat tattaaatga aatgctaaca gcaacaccga ttaacatttg
     3841 ggtatcaaca ccatcattta tggaaatgtg tttattatta ccaacgctta atgaagaaca
     3901 atatggtagt cttaacgaat tcttcttctg tggtgaaatt ctacctcaca gagcagcaaa
     3961 agcgttagtg agccgtttcc caagtgcgac gatttacaac acatatggtc caactgaagc
     4021 tacggtagca gttacaagta ttcaaattac acaagaaatc ttagatcaat atccgacatt
     4081 acctgttggc gttgaaagac taggcgcaag attatctact acagatgacg gtgaacttgt
     4141 tattgaaggt caaagtgtaa gtttaggata cttaaaaaat gaccaaaaaa cagctgaagt
     4201 atttaatttc gatgacggta ttcgtacata tcacactggt gataaagcga agtttgaaaa
     4261 tggtcaatgg ttcattcaag gtcgtattga tttccaaatc aaattgaatg gctacagaat
     4321 ggaattagaa gaaattgaaa cacaattacg ccagtctgag ttcgtaaaag aagcgattgt
     4381 tgtacctgta tataaaaatg ataaagttat tcatttaatt ggtgcaattg tgccaacgac
     4441 tgaagttacg gacaatgcag aaatgactaa aaatattaaa aatgacttga aatcacgctt
     4501 accagagtat atgattccta gaaagtttga atggatggaa caattgccat tgacttcaaa
     4561 tggtaaaatt gacagaaaga aaattgcaga ggtaattaac ggatgattcc atatggtgat
     4621 tttacattct tcttaattgc tttaattgca ttattaccag tcattatact tggattttta
     4681 ggtaagcgaa gttacattta taatggcgta gttacagcat ttatgattgt gttaatcttt
     4741 tcttctgata aacataatct gtttgaccaa aagtatttaa gtgttcaatt aattagtttt
     4801 attatttacg tcgtatggca agttttattg ataatgtttt attatcattc aaaaccaaaa
     4861 aataattcat tttcaaaatt tgtaactgta atggttttat caatattgcc attagcactt
     4921 gtgaaagtgt tacaaagtac atggttaggt ggacatcaga ttcacttcca tgaaagtaaa
     4981 ttaattgaat ttgttggttt cttaggaatt tcttatgtta cattcaaaag tgtgcagtta
     5041 attatggaaa ttcgtgatgg ttctatcaaa gaaattaaag tatggaaatt aattcaattt
     5101 atttcattct tcccaacgat ttcatctgga ccaatcgatc gttacaaacg tttcgttaaa
     5161 gacgataaaa aagtaccaac aggcaatgaa tatcgtgaat tagtattaaa agcaattcac
     5221 atgattatgc ttggtttctt gtataaatat attgttgctt actttattaa cacatatgca
     5281 atcatgccgt tacaattaga cttacatggc tttgtcaatt tgtggttata tatgtacgca
     5341 tacagcttat atttattctt tgactttgca ggttatagtt tatttgcgat agcatttagt
     5401 tatttattcg gtattaaaac accaccaaac ttcgataaac ctttcaaagc gaaaaatatt
     5461 aaagatttct ggaatagatg gcatatgaca ttatcattct ggttcagaga ttgtatttac
     5521 atgagatctt tattctacat gtctcgtaaa aaattattga agagtcaatt tgcaatgtct
     5581 aacgtggcat tcttaatcaa cttcttcata atgggaattt ggcatggtat cgaagtgtat
     5641 tacattgttt atggtttata ccatgcagca ttgtttatag gttatggcta ttatgaacgt
     5701 tggcgtaaga aacatccgcc acgttggcaa aatggtttca caacagcact tagcattgtg
     5761 attacattcc actttgtaac atttggcttt ttaatcttct caggtaaact tatataataa
     5821 aggagaattt aattatggaa tttagagaac aagtattaaa tttattagca gaagtagcag
     5881 aaaatgatat tgtaaaagaa aatccagacg tagaaatttt tgaagaaggt attattgatt
     5941 ctttccaaac agttggatta ttattagaga ttcaaaataa acttgatatc gaagtatcta
     6001 ttatggactt tgatagagat gagtgggcaa caccaaataa aatcgttgaa gcattagaag
     6061 agttacgatg aaattaaaac cttttttacc cattttaatt agtggagcgg tattcattgt
     6121 ctttctatta ttacctgcta gttggtttac aggattagta aatgaaaaga ctgtagaaga
     6181 taatagaact tcattgacag atcaagtact aaaaggcaca ctcattcaag ataagttata
     6241 cgaatcaaac aagtattatc ctatatacgg ctctagtgaa ttaggtaaag atgacccatt
     6301 taatcctgca attgcattaa ataagcataa cgccaacaaa aaagcattct tattaggtgc
     6361 tggtggttct acagacttaa ttaacgcagt tgaacttgca tcacagtatg ataaattaaa
     6421 aggtaagaaa ttaacattta ttatttcacc acaatggttt acaaaccatg gtttaacgaa
     6481 tcaaaacttt gatgctcgta tgtctcaaac tcaaattaat caaatgttcc agcagaaaaa
     6541 catgtctact gaattaaaac gtcgttatgc acaacgttta ttacagtttc cacatgtaca
     6601 caataaagaa tacttgaaat cttatgctaa aaaccctaaa gaaactaaag atagttatat
     6661 ttctggtttt aaagagaatc aattgattaa aatagaagcg attaaatcat tgtttgcaat
     6721 ggataaatct ccattagaac atgttaaacc tgctacaaaa ccagacgctt cttgggatga
     6781 gatgaaacaa aaagcagttg aaattggtaa agctgatact acatcgaata aatttggtat
     6841 tagagatcaa tactggatat taattcaaga aagtaagcgt aaagttagac gtgactacga
     6901 attcaatgtt aattctccag aattccaaga tttagaatta cttgtaaaaa caatgcgtgc
     6961 tgctggtgca gatgttcaat atgtaagtat tccatcaaac ggtgtatggt atgaccacat
     7021 tggtatcgat aaagaacgtc gtcaagcagt ttataaaaaa atccattcta ctgttgtaga
     7081 taatggtggt aaaatttacg atatgactga taaagattat gaaaaatatg ttatcagtga
     7141 tgccgtacac atcggttgga aaggttgggt ttatatggat gagcaaattg cgaaacatat
     7201 gaaaggtgaa ccacaacctg aagtagataa acctaaaaat taaaatacaa atagcacata
     7261 actcaacgat tttgattgag cgtatgtgct atttttatat tttaaat
//
LOCUS       DANBWH010000138         7243 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.142, whole genome shotgun sequence.
ACCESSION   DANBWH010000138 DANBWH010000000
VERSION     DANBWH010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7243
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.142"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..137)
                     /locus_tag="PPY92_001976"
     CDS             complement(<1..137)
                     /locus_tag="PPY92_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDJ2625964.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYTLSYRDISEILRERGVNVHH
                     ST"
     gene            191..562
                     /locus_tag="PPY92_001977"
                     /pseudo
     CDS             191..562
                     /locus_tag="PPY92_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016912861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase family
                     protein"
     gene            1399..2142
                     /locus_tag="PPY92_001978"
     CDS             1399..2142
                     /locus_tag="PPY92_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HDJ2625965.1"
                     /translation="MLTVYGHRGLPSKAPENTIASFKAASEVEGINWLELDVAITKDE
                     QLIIIHDDYLERTTNMSGEITELNYDEIKDASAGSWFGEKFKDEHLPTFDDVVKIANE
                     YNMNLNVELKGITGPNGLALSKSMVKQVEEQLTNLNQNQEVLISSFNVVLVKLAEEIM
                     PQYNRAVIFHTTSFREDWRTLLDYCNAKIVNTEDAKLTKAKVKMVKEAGYELNVWTVN
                     KPARANQLANWGVDGIFTDNADKMVHLSQ"
     gene            2239..2667
                     /locus_tag="PPY92_001979"
     CDS             2239..2667
                     /locus_tag="PPY92_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HDJ2625966.1"
                     /translation="MKYDDFIVGETFKTKSLHITEEEIIQFATTFDPQYMHIDKEKAE
                     QSRFKGIIASGMHTLSISFKLWVEEGKYGEEVVAGTQMNNVKFIKPVYPGNTLYVIAE
                     ITNKKSIKKENGLVTVSLSTYNENEEIVFKGEVTALINNS"
     gene            complement(2713..4722)
                     /gene="mecA"
                     /locus_tag="PPY92_001980"
     CDS             complement(2713..4722)
                     /gene="mecA"
                     /locus_tag="PPY92_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase MecA"
                     /protein_id="HDJ2625967.1"
                     /translation="MMKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFK
                     QVYKDSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKI
                     KTNYGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQSIHIENLKSERGKILDRNN
                     VELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTV
                     KKMDEYLSDFAKKFHLTTNETESRNYPLGKATSHLLGYVGPINSEELKQKEYKGYKDD
                     AVIGKKGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQ
                     KSIYNNMKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPL
                     LNKFQITTSPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV
                     NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKN
                     LDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISK
                     ENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKD
                     NPNMMMAINVKDVQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
     gene            4819..6576
                     /gene="mecR1"
                     /locus_tag="PPY92_001981"
     CDS             4819..6576
                     /gene="mecR1"
                     /locus_tag="PPY92_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer MecR1"
                     /protein_id="HDJ2625968.1"
                     /translation="MLSSFLMLSIISSLLTICVIFLVRMLYIKYTQNIMSHKIWLLVL
                     VSTLIPLIPFYKISNFTFSKDMMNRNVSDTTSSVSHMLDGQQSSVTKDLAINVNQFET
                     SNITYMILLIWVFGSLLCLFYMIKAFRQIDVIKSSSLESSYLNERLKVCQSKMQFYKK
                     HITISYSSNIDNPMVFGLVKSQIVLPTVVVETMNDKEIEYIILHELSHVKSHDLIFNQ
                     LYVVFKMIFWFNPALYISKTMMDNDCEKVCDRNVLKILNRHEHIRYGESILKCSILKS
                     QHINNVAAQYLLGFNSNIKERVKYIALYDSMPKPNRNKRIVAYIVCSISLLIQAPLLS
                     AHVQQDKYETNVSYKKLNQLAPYFKGFDGSFVLYNEREQAYSIYNEPESKQRYSPNST
                     YKIYLALMAFDQNLLSLNHTEQQWDKHQYPFKEWNQDQNLNSSMKYSVNWYYENLNKH
                     LRQDEVKSYLDLIEYGNEEISGNENYWNESSLKISAIEQVNLLKNMKQHNMHFDNKAI
                     EKVENSMTLKQKDTYKYVGKTGTGIVNHKEANGWFVGYVETKDNTYYFATHLKGEDNA
                     NGEKAQQISERILKEMELI"
     gene            6576..6947
                     /gene="mecI"
                     /locus_tag="PPY92_001982"
     CDS             6576..6947
                     /gene="mecI"
                     /locus_tag="PPY92_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mecA-type methicillin resistance repressor MecI"
                     /protein_id="HDJ2625969.1"
                     /translation="MDNKTYEISSAEWEVMNIIWMKKYASANNIIEEIQMQKDWSPKT
                     IRTLITRLYKKGFIDRKKDNKIFQYYSLVEESDIKYKTSKNFINKVYKGGFNSLVLNF
                     VEKEDLSQDEIEELRNILNKK"
     gene            complement(7032..7100)
                     /gene="psm-mec"
                     /locus_tag="PPY92_001983"
     CDS             complement(7032..7100)
                     /gene="psm-mec"
                     /locus_tag="PPY92_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-mec"
                     /protein_id="HDJ2625970.1"
                     /translation="MDFTGVITSIIDLIKTCIQAFG"
BASE COUNT         2597 a         1032 c         1150 g         2464 t
ORIGIN      
        1 gttgaatgat gaacgtttac accacgttcc cttaatattt cagatatatc acgataactc
       61 aatgtatatc ttagatagta gccaacggct acagtgataa catccttgtt aaattgttta
      121 tatctgaaat agttcataca gaagactcct ttttgttaaa attatactat aaattcaact
      181 ttgcaacaga accgtattat ggaatagaga tgttggtaac atttatacag gatcattata
      241 cttaagttta atttcgttat tacagaacca cacattccaa ccagaagaga aagtatgtct
      301 atttagttat ggttcaggag cagtaggaga aatctttagt ggttcaatcg ttaaaggata
      361 tgacaaagca ttagataaag agaaacactt aaatatgcta gaatctagag agcaattatc
      421 agtcgaagaa tacgaaacat tctttaacag atttgataat caagaatttg atttcgaacg
      481 tgaattgaca caagatccat attcaaaagt atacttatac agtatagaag accatatcag
      541 aacatataag atagagaaat aaactagtgg ccgattgtgc ttgatgagct tgggacataa
      601 atcctaactc gaaataaata agcatatcac taaactgatt ttttaaagtt tacagtgata
      661 tgcttatttt tttatcttac gattttgtac gtgcatgctt gcctaggggt atggctcgag
      721 ccattagtct ctcgcacata ctattccctc aggcgtcagc acttacaaaa tcggttgtaa
      781 ttttcatttt tatacgcatt cttactgaga ttatactaat aagaggaata gtaaaagcaa
      841 ttctaagtaa aattgcagat aagaggtttg ttaaaagcag ttctaagtaa aattgcagat
      901 aagaggtttg ttaaaagcag ttctaagtaa aattgcagat aagaggtttg ttaaaagcag
      961 ttctcagtaa aattacagat aagaggtacg ttaaaagcag ttctaagtaa aattgcagat
     1021 aagaggtacg ttaaaagcag ttctaggcaa aattgcagat aagaggtacg ttaaaagcag
     1081 ttctcagtaa aattgctgat aaggggtaag ttaaaagcaa tcctaagtaa aattgcagat
     1141 aagaggtaag ttaaaagcaa tcctaagtaa aattgcagat aaggggtaca gaaaaactag
     1201 acttgattac aaaatggagc ttgggacata aatgattttt taaaaatgag atgagacgta
     1261 gattaactcc ataatcaata cgaatctatc gacttcttta tttatgatat tcatctcttt
     1321 ttaatggaaa taaaagtgcg attaatgtga taatacagtt acgttaatta aaaaaataaa
     1381 aatgcaagga gaggtaatat gctaactgta tatggacata gaggattacc tagtaaagct
     1441 ccggaaaata caattgcatc atttaaagct gcttcagaag tagaaggtat aaactggttg
     1501 gagttagatg ttgcaattac aaaagatgaa caactgatta tcattcatga tgattattta
     1561 gaacggacta caaatatgtc cggggaaata actgaattga attatgatga aattaaagat
     1621 gcttctgcag gatcttggtt tggtgaaaaa ttcaaagatg aacatttgcc aactttcgat
     1681 gatgtagtaa aaatagcaaa tgaatataat atgaatttaa atgtagaatt aaaaggtatt
     1741 actggaccga atggactagc actttctaaa agtatggtta agcaagtgga agaacaatta
     1801 acaaacttaa atcagaatca agaagtgctc atttcaagct ttaatgttgt gcttgttaaa
     1861 cttgcagaag aaatcatgcc acaatataac agagcagtta tattccatac aacttcgttt
     1921 cgtgaagact ggagaacact tttagattac tgtaatgcta aaatagtaaa cactgaagat
     1981 gccaaactta ctaaagcaaa agtaaaaatg gtaaaagaag cgggttatga attgaacgta
     2041 tggactgtaa acaaaccagc acgtgcaaac caacttgcta attggggagt tgatggtatc
     2101 tttacagaca atgcagataa aatggtgcat ttgtctcaat agaaagttag aggtgagtct
     2161 tacgtttcag tgacggtaga cttaccttta acatgttaca tactaaaaaa ttaatttgaa
     2221 taagaaagag agacatatat gaaatacgat gattttatag taggagaaac attcaaaaca
     2281 aaaagccttc atattacaga agaagaaatt atccaatttg caacaacttt tgatcctcaa
     2341 tatatgcata tagataaaga aaaagcagaa caaagtagat ttaaaggtat cattgcatct
     2401 ggcatgcata cactttcaat atcatttaaa ttatgggtag aagaaggtaa atacggagaa
     2461 gaagttgtag caggaacaca aatgaataac gttaaattta ttaaacctgt atacccaggt
     2521 aatacattgt acgttatcgc tgaaattaca aataagaaat ccataaaaaa agaaaatgga
     2581 ctcgttacag tgtcactttc aacatacaat gaaaatgaag aaattgtatt taagggagaa
     2641 gtaacagcac ttattaataa ttcataataa aacagtgaag caaccatcgt tacggattgc
     2701 ttcactgttt tgttattcat ctatatcgta ttttttatta ccgttctcat atagctcatc
     2761 atacacttta cctgagattt tggcattgta gctagccatt cctttatctt gtacatcttt
     2821 aacattaata gccatcatca tgtttggatt atctttatca tatgatataa accacccaat
     2881 ttgtctgcca gtttctcctt gtttcatttt gagttctgca gtaccggatt tgccaattaa
     2941 gtttgcataa gatctataaa tatcttcttt atgtgtttta tttacgactt gttgcatacc
     3001 atcagttaat agattgatat tttctttgga aataatattt ttcttccaaa ctttgttttt
     3061 cgtgtctttt aataagtgag gtgcgttaat attgccatta ttttctaatg cgctatagat
     3121 tgaaaggatc tgtactgggt taatcagtat ttcaccttgt ccgtaacctg aatcagctaa
     3181 taatatttca ttatctaaat ttttgtttga aatttgagca ttataaaatg gataatcact
     3241 tggtatatct tcaccaacac ctagtttttt catgcctttt tcaaatttct tactgcctaa
     3301 ttcgagtgct actctagcaa agaaaatgtt atctgatgat tctattgctt gttttaagtc
     3361 gatattacca tttaccactt catatcttgt aacgttgtaa ccaccccaag atttatcttt
     3421 ttgccaacct ttaccatcga ttttataact tgttttatcg tctaatgttt tgttatttaa
     3481 cccaatcatt gctgttaata ttttttgagt tgaacctggt gaagttgtaa tctggaactt
     3541 gttgagcaga ggttcttttt tatcttcggt taatttatta tattcttcgt tactcatgcc
     3601 atacataaat ggatagacgt catatgaagg tgtgcttaca agtgctaata attcacctgt
     3661 ttgagggtgg atagcagtac ctgagccata atcatttttc atgttgttat aaatactctt
     3721 ttgaacttta gcatcaatag ttagttgaat atctttgcca tcttttttct ttttctctat
     3781 taatgtatgt gcgattgtat tgctattatc gtcaacgatt gtgacacgat agccatcttc
     3841 atgttggagc tttttatcgt aaagtttttc gagtcccttt ttaccaataa ctgcatcatc
     3901 tttatagcct ttatattctt tttgttttaa ttcttcagag ttaatgggac caacataacc
     3961 taatagatgt gaagtcgctt ttcctagagg atagttacga ctttctgttt cattagttgt
     4021 aagatgaaat ttttttgcga aatcacttaa atattcatcc atttttttaa cggttttaag
     4081 tggaacgaag gtatcatctt gtacccaatt ttgatccatt tgttgtttga tatagtcttc
     4141 agaaatactt agttctttag cgattgcttt ataatctttt ttagatacat tctttggaac
     4201 gatgcctatc tcatatgctg ttcctgtatt ggccaattcc acattgtttc ggtctaaaat
     4261 tttaccacgt tctgatttta aattttcaat atgtatgctt tggtctttct gcattcctgg
     4321 aataatgacg ctatgatccc aatctaactt ccacatacca tcttctttaa caaaattaaa
     4381 ttgaacgttg cgatcaatgt taccgtagtt tgttttaatt ttatattgag catctactcg
     4441 ttttttattt ttagatactt tttttatttt acgatcctga atgtttatat ctttaacgcc
     4501 taaactatta tatattttta tcggacgttc agtcatttct acttcaccat tatcgctttt
     4561 agaaatataa ctgctatctt tataaacttg tttgaaattt ttatcttcaa ttgcatcaat
     4621 agtattatta atttctttat cttttgaagc ataaaaatat ataccaaacc cgacaactac
     4681 aactattaaa ataagtggaa caatttttat ctttttcatc aatatcctcc ttatataaga
     4741 ctacattttt agtatattac aaatgtagta tttatgtcaa aataatgtta taatttttgt
     4801 gatatggagg tgtagaaggt gttatcatct tttttaatgt taagtataat cagttcattg
     4861 ctcacgatat gtgtaatttt tttagtgaga atgctctata taaaatatac tcaaaatatt
     4921 atgtcacata agatttggtt attagtgctc gtctccacgt taattccatt aataccattt
     4981 tacaaaatat cgaattttac attttcaaaa gatatgatga atcgaaatgt atctgacacg
     5041 acttcttcgg ttagtcatat gttagatggt caacaatcat ctgttacgaa agacttagca
     5101 attaatgtta atcagtttga gacctcaaat ataacgtata tgattctttt gatatgggta
     5161 tttggtagtt tgttgtgctt attttatatg attaaggcat tccgacaaat tgatgttatt
     5221 aaaagttcgt cattggaatc gtcatatctt aatgaacgac ttaaagtatg tcaaagtaag
     5281 atgcagttct acaaaaagca tataacaatt agttatagtt caaacattga taatccgatg
     5341 gtatttggtt tagtgaaatc ccaaattgta ctaccaactg tcgtagtcga aaccatgaat
     5401 gacaaagaaa ttgaatatat tattctacat gaactatcac atgtgaaaag tcatgactta
     5461 atattcaacc agctttatgt tgtttttaaa atgatattct ggtttaatcc tgcactatat
     5521 ataagtaaaa caatgatgga caatgactgt gaaaaagtat gtgatagaaa cgttttaaaa
     5581 attttgaatc gccatgaaca tatacgttat ggtgaatcga tattaaaatg ctctatttta
     5641 aaatctcagc acataaataa tgtggcagca caatatttac taggttttaa ttcaaatatt
     5701 aaagaacgtg ttaagtatat tgcactttat gattcaatgc ctaaacctaa tcgaaacaag
     5761 cgtattgttg cgtatattgt atgtagtata tcgcttttaa tacaagcacc gttactatct
     5821 gcacatgttc aacaagacaa atatgaaaca aatgtatcat ataaaaaatt aaatcaacta
     5881 gctccgtatt tcaaaggatt tgatggaagt tttgtgcttt ataatgaacg ggagcaagct
     5941 tattctattt ataatgaacc agaaagtaaa caacgatatt cacctaattc tacttacaaa
     6001 atttatttag cgttaatggc attcgaccaa aatttactct cattaaatca tactgaacaa
     6061 caatgggata aacatcaata tccatttaaa gaatggaacc aagatcaaaa tttaaattct
     6121 tcaatgaaat attcagtaaa ttggtattac gaaaatttaa acaaacattt aagacaagat
     6181 gaggttaaat cttatttaga tctaattgaa tatggtaatg aagaaatatc agggaatgaa
     6241 aattattgga atgaatcttc attaaaaatt tctgcaatag aacaggttaa tttgttgaaa
     6301 aatatgaaac aacataacat gcattttgat aataaggcta ttgaaaaagt tgaaaatagt
     6361 atgactttga aacaaaaaga tacttataaa tatgtaggta aaactggaac aggaatcgtg
     6421 aatcacaaag aagcaaatgg atggttcgta ggttatgttg aaacgaaaga taatacgtat
     6481 tattttgcta cacatttaaa aggcgaagac aatgcgaatg gcgaaaaagc acaacaaatt
     6541 tctgagcgta ttttaaaaga aatggagtta atataatgga taataaaacg tatgaaatat
     6601 catctgcaga atgggaagtt atgaatatca tttggatgaa aaaatatgca agtgcgaata
     6661 atataataga agaaatacaa atgcaaaagg actggagtcc aaaaaccatt cgtacactta
     6721 taacgagatt gtataaaaag ggatttatag atcgtaaaaa agacaataaa atttttcaat
     6781 attactctct tgtagaagaa agtgatataa aatataaaac atctaaaaac tttatcaata
     6841 aagtatacaa aggcggtttc aattcacttg tcttaaactt tgtagaaaaa gaagatctat
     6901 cacaagatga aatagaagaa ttgagaaata tattgaataa aaaataaaat tgttgtgttt
     6961 acaacaatac atagaaaaca gaggaaacaa tcaagtcgtt gaatatttcc tctgtttttt
     7021 agttgaaaaa attaaccgaa agcctgaatg caagtcttga ttaaatcaat aatgcttgta
     7081 ataacaccag tgaaatccat atgcataccc tctttctatt taagatacat taggtataat
     7141 atcaaacaaa taaaaaatgt taaaaattcc ctaattggct atttagattg cataaatgtc
     7201 aaaaatttga aaaacataca acgactttgc ataaaaaatc gtc
//
LOCUS       DANBWH010000139         7186 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.143, whole genome shotgun sequence.
ACCESSION   DANBWH010000139 DANBWH010000000
VERSION     DANBWH010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7186
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.143"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            47..1087
                     /locus_tag="PPY92_001984"
     CDS             47..1087
                     /locus_tag="PPY92_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDJ2625971.1"
                     /translation="MIIAIIILIFISFFFSGSETALTAANKTKFKTEADKGDKKAKGI
                     VKLLEKPSEFITTILIGNNVANILLPTLVTIMALRWGISVGIASAVLTVVIILISEVI
                     PKSVAATFPDKITRLVYPIINICVIVFRPITLLLNKLTDSINRSLSKGQPQEHQFSKE
                     EFKTMLAIAGHEGALNEIETSRLEGVINFENLKVKDVDTTPRINVTAFASNATYEEVY
                     ETVMNKPYTRYPVYEGDIDNIIGVFHSKYLLAWSNKKEDQITNYSAKPLFVNEHNKAE
                     WVLRKMTISRKHLAIVLDEFGGTEAIVSHEDLIEELLGMEIEDEMDKKEKEKLSQQQI
                     QFQQRKNRNVSI"
     gene            1101..2168
                     /locus_tag="PPY92_001985"
     CDS             1101..2168
                     /locus_tag="PPY92_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HDJ2625972.1"
                     /translation="MWNKNRLTQMLSIEYPIIQAGMTGSTTPKLVASVSNSGGLGTIG
                     AGYFNTQQLEDEIDYVRQLTSNSFGVNVFVPSQQSYTSSQIENMNAWLKPYRRALHLE
                     EPVVKIIEEQQFKCHIDTIIKKQVPVCCFTFGIPNESIIERLKEANIKLIGTATSVDE
                     AIANEKAGMDAIVAQGSEAGGHRGSFLKPKNQLPMVGTISLVPQIVDVVSIPVIAAGG
                     IMDGRGVLASIVLGAEGVQMGTAFLTSQDSNASELLRDAIINSKETDTVVTKAFSGKL
                     ARGINNRFIEEMSQYEGDIPDYPIQNELTSSIRKAAANIGDKELTHMWSGQSPRLATT
                     HPANTIMSNIINQINQIMQYK"
     gene            2422..2505
                     /locus_tag="PPY92_001986"
     CDS             2422..2505
                     /locus_tag="PPY92_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625973.1"
                     /translation="MIIIKRKHQAEDRRKILIKRKHQIKVR"
     gene            2553..3401
                     /locus_tag="PPY92_001987"
     CDS             2553..3401
                     /locus_tag="PPY92_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDJ2625974.1"
                     /translation="MINIGLTGWGDHYSLYEDLERQTDKLKTYAGHFPVVELDATYYA
                     IQPERNILKWIKETPDTFEFVVKIHQALTLHADYKTFADTRQELFDQFKNMLEPLHTQ
                     KKLAMVLVQFPPWFDCNAQNIKYILYVRQQLQAFPMCVEFRHQSWFSDAFKEQTLAFL
                     TEHQIIHAVVDEPQVKDGSVPLVNRITNEIAFVRYHGRNHYGWTKKDMSDQEWRDVRY
                     LYDYNEQELIDLAQKAQILAQKAKKVYVIFNNNSGGHAANNAKTYQRLLNIEYEGLAP
                     QQLKLF"
     gene            3414..4241
                     /locus_tag="PPY92_001988"
     CDS             3414..4241
                     /locus_tag="PPY92_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDJ2625975.1"
                     /translation="MLLTITLLVLIGGLSAIIGSIVGIGGGIIIVPTMVYLGVEHGLL
                     HNITTQVAIGTSSVILIVTGLSSSLGYLKTKQVDIKNGSIFLFGLLPGSLLGSFISRY
                     LTFESFNLYFGIFLIFVAILLMVRNKIKPFKIFDKPKYEKTYVDAKGKTYHYSVPPLF
                     AFITTFLIGILTGLFGIGGGALMTPLMLIVFRFPPHVAVGTSMMMIFFSSVMSSIGHI
                     AQGHVAWGYAIILIISSYFGAKIGVKVNQSIKSDTVVTLLRTVMLLMGIYLIIRALI"
     gene            4268..5587
                     /locus_tag="PPY92_001989"
     CDS             4268..5587
                     /locus_tag="PPY92_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDJ2625976.1"
                     /translation="MRLTIYHTNDIHSHLHEYERIKVYMAEQRPRLNHPSLYVDLGDH
                     VDLSAPITEATLGKKNVALLNEAKCDVATIGNNEGMTISHEALNHLYDEAKFIVTCSN
                     VIDESGHLPNNIVSSYIKDMDGVKILFVAATAPFTPFYRALDWIVTDPLESIKEEIKH
                     QRGKFDVIIVLSHCGIFFDETLCQELPEIDVIFGSHTHHYFEHGEINNGVLMAAAGKY
                     GNYLGEVNLTFEAHKVVHKTAKIIPLETLPEVETSFEEEGKTLMSNSVIQHPVVLKRS
                     MNHITEAAYLLAQSVCEYTHAQCAIINAGLLVKDIVKDEVTEYDIHQMLPHPINMVRV
                     RLSGVKLKEIITKSNKQEYMYEHAQGLGFRGNIFGGYILYNLGYIHSTGRYYLNGEEI
                     EDDKEYVLGTIDMYTFGRYFPTLKELPKEYLMPEFLRDIFKEKLLEY"
     gene            5671..6576
                     /gene="lipA"
                     /locus_tag="PPY92_001990"
     CDS             5671..6576
                     /gene="lipA"
                     /locus_tag="PPY92_001990"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDJ2625977.1"
                     /translation="MATKNEEILRKPDWLKIKLNTNENYTGLKKMMREKNLNTVCEEA
                     KCPNIHECWGARRTATFMILGAVCTRACRFCAVKTGLPNELDLNEPERVAESVELMNL
                     KHVVITAVARDDLRDAGSNVYAETVRKVRERNPFTTIEILPSDMGGDYDALETLMASR
                     PDILNHNIETVRRLTPRVRARATYDRTLEFLRRSKELQPDIPTKSSIMVGLGETIEEI
                     YETMDDLRANDVDILTIGQYLQPSRKHLKVQKYYTPLEFGKLRKVAMDKGFKHCQAGP
                     LVRSSYHADEQVNEAAKEKQRQGEA"
     gene            6718..7101
                     /locus_tag="PPY92_001991"
     CDS             6718..7101
                     /locus_tag="PPY92_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HDJ2625978.1"
                     /translation="MIKVDQHYFELIENYRECFNEEQFIARYSDILDKYDYIVGDYGY
                     DQLRLKGFYKDSNKKAEMSKRFSNIQDYIFEYCNFGCPYFVLRHLSKQEVKKLIEEVH
                     PSDVIDDDNKLQDVKIKPTIQDTEH"
BASE COUNT         2589 a         1041 c         1381 g         2175 t
ORIGIN      
        1 agtaactaca ataaaaataa gatatgacaa taaggagact acacgcgtga tcattgccat
       61 aattatattg atatttattt cgtttttctt ttcaggaagc gagacggcat taacggctgc
      121 caataaaaca aaatttaaaa ctgaagctga caaaggtgat aaaaaagcaa aaggcattgt
      181 aaagttactt gaaaaaccaa gtgagtttat tacaacgatt ctaattggga ataatgtcgc
      241 gaatatttta ttaccaacac ttgttacaat tatggcttta cgttggggga ttagcgttgg
      301 tattgcatca gctgttttaa cagttgttat cattttgatt tccgaagtga ttcccaagtc
      361 tgtcgctgca acatttccag ataaaataac aaggcttgta tatccaatta ttaatatttg
      421 tgtcattgtg tttcgcccta tcacattact tttaaataag ttgacggaca gtattaatcg
      481 aagtttatct aagggtcaac ctcaagaaca tcaattttca aaagaagaat ttaaaacaat
      541 gttagcaatt gctggacatg aaggtgcttt aaatgaaatt gagacgagta ggttggaagg
      601 tgtcattaat tttgaaaatt taaaagtaaa agatgtagat acaacaccta gaattaatgt
      661 gacggcattt gcttcaaatg cgacatacga agaagtttat gaaacggtta tgaataagcc
      721 atacactaga tatccagtgt acgagggaga tattgataac attattgggg tgtttcattc
      781 taaatatctg ttggcttgga gtaataaaaa agaagatcaa attacaaact attcagctaa
      841 gccattattt gtgaatgaac acaataaagc tgaatgggta ttacgtaaga tgactatttc
      901 tagaaaacat ttagcaattg tgttggacga atttggtggt accgaagcga tagtgtcaca
      961 tgaagactta attgaagaat tattaggtat ggaaattgaa gatgagatgg ataaaaagga
     1021 aaaagaaaaa ctttctcaac agcaaattca atttcaacaa cggaaaaatc gcaacgtatc
     1081 tatataagga gcgaacagct atgtggaata agaatcgact tactcaaatg ttaagtattg
     1141 aatatccaat tatacaagca ggtatgacag gaagtacgac accgaaatta gttgcatcag
     1201 taagtaacag tggtgggtta ggcacaatag gcgcaggtta ctttaatacg cagcaattgg
     1261 aagatgaaat agattatgta cgccaattaa cgtcaaattc ttttggcgta aatgtctttg
     1321 taccaagtca acaatcatat accagtagtc aaattgaaaa tatgaatgca tggttaaaac
     1381 cttatcgacg cgcattacat ttagaagagc cggttgtaaa aattatcgaa gaacaacaat
     1441 ttaagtgtca tattgatacg ataattaaaa agcaagtgcc tgtatgttgt tttacttttg
     1501 gaattccaaa tgaatctatt atagaaagat tgaaagaagc aaacattaag ctaataggta
     1561 cagcaacaag tgttgatgaa gctattgcga atgaaaaagc gggtatggat gctatcgttg
     1621 ctcaaggtag tgaagcaggt ggacatcgtg gttcattttt aaaacctaaa aatcaattac
     1681 ctatggttgg aacaatatct ttagtgccac aaattgtaga tgtcgtttca attccggtca
     1741 ttgccgctgg tggaattatg gacggtagag gagttttggc aagtattgtc ttaggtgcag
     1801 aaggggtaca aatgggcact gcatttttaa catcacaaga tagtaatgca tcagaactac
     1861 tacgagacgc aattataaat agtaaagaaa cagatacagt cgttacaaaa gcgtttagtg
     1921 gaaagcttgc acgcggtatc aacaataggt ttatcgaaga aatgtcccaa tacgaaggcg
     1981 atatcccaga ttatccaata caaaatgagc taacaagtag cataagaaaa gccgcagcaa
     2041 acatcggcga caaagagtta acacatatgt ggagtggaca aagcccgcga ctagcaacaa
     2101 cgcatcccgc caacaccatc atgtccaata taatcaatca aattaatcaa atcatgcaat
     2161 ataaataatc gaccgcaatc cacaaaagca caagcactcc caaacattat tttagtgctt
     2221 gccatttttg tggattgcgt ttctatttta ccaatctagt caaacgaaat tatcaaacta
     2281 aagttcgctg aaatgattat agtcaaacga aaacatcaaa ctaaagttcg ctgaaatgat
     2341 tatgatcaaa cgaaattatc gagctgaagg tcaccgaaat gattataatt aaacgaaatt
     2401 atcgagctaa agttcactga aatgattata atcaaacgaa aacatcaagc tgaagatcgc
     2461 cgaaagattt taatcaagcg aaaacatcaa attaaagttc gctgaaatga ttatgataaa
     2521 agttatatgg tatgatgaca ttggtgatat atatgataaa catcggatta acaggttggg
     2581 gtgatcacta ttcattatat gaagatttag aacgccaaac cgataaactt aaaacatatg
     2641 ctggacattt tccggttgtc gaattagatg cgacatacta tgcgatacaa ccggaaagaa
     2701 atatattgaa atggataaaa gaaacgcctg atacatttga atttgtggtc aaaattcatc
     2761 aagcactcac attgcatgca gactacaaaa catttgcaga tacaaggcaa gaactatttg
     2821 atcaatttaa gaatatgtta gagcccttac atacacagaa aaaattagca atggtattgg
     2881 ttcaatttcc gccatggttt gactgcaatg cacaaaatat caaatatatt ttgtatgtaa
     2941 gacagcaatt acaagcattt ccaatgtgtg tagaatttag gcatcaatca tggtttagtg
     3001 atgcatttaa agaacaaaca ttggcatttt taacagaaca tcaaatcatt catgcagtag
     3061 ttgatgaacc acaagtgaaa gatggcagtg tacctttagt caatcgaatc acaaatgaaa
     3121 ttgcgtttgt acgttatcat ggacgtaatc attacggttg gactaagaaa gatatgtcag
     3181 atcaagaatg gcgagatgta cgctatttat atgattataa tgagcaagaa ttaatagact
     3241 tggcacaaaa ggcacaaata ttagcacaaa aagctaagaa agtttacgtc atatttaaca
     3301 ataattctgg tggtcatgca gcaaataatg ccaaaacata tcagcgatta ttgaatatag
     3361 aatatgaagg gttagcacca caacaattaa aattatttta agaggcgacg actatgttat
     3421 taacaattac attattagtt ttaatcggag gtttgtcagc gattataggg tctatcgtag
     3481 gcattggagg cggtattatt atcgttccaa caatggttta cctcggtgtt gaacatggat
     3541 tactacataa tattacaaca caagtagcga tagggacgtc ttcagtcatt ctaattgtga
     3601 caggactttc ttcatcactt ggatatttaa aaacaaaaca agttgatatt aaaaatggtt
     3661 ccatcttttt atttggacta ttaccaggtt cattgcttgg gtccttcatt agtagatatt
     3721 taacatttga gtcatttaat ttatattttg gtatcttttt aattttcgta gccattttat
     3781 taatggtaag aaataagatt aaaccgttta aaattttcga taaacccaag tatgaaaaga
     3841 cttatgtaga cgctaaaggt aaaacatatc attatagtgt gccaccattg tttgctttta
     3901 ttacaacgtt tttaattggt atattgacag gtttatttgg tattggaggt ggcgcactaa
     3961 tgacgccact aatgcttatt gtatttagat ttccacctca tgtagctgtt ggaacaagta
     4021 tgatgatgat tttcttttca agtgtcatga gttctatagg gcacattgct caaggtcacg
     4081 tagcttgggg ttatgcaatc attttaatta tttctagtta ttttggtgcg aaaatcggtg
     4141 tcaaagtgaa tcaatcaatt aagtcagata cggtagtaac attattgaga acagtaatgt
     4201 tgttaatggg tatatattta attattcgag cgttgattta atacaacttt aaaaggagga
     4261 cgtcaatttg aggcttacaa tttatcatac gaacgatatt catagtcatt tacatgaata
     4321 cgaacgcatt aaagtatata tggcagaaca acggccacga cttaatcatc cttctttata
     4381 tgttgatcta ggtgatcatg tagatttatc cgcacctata actgaagcaa ctttaggtaa
     4441 aaagaatgtg gcattactaa atgaagcaaa atgtgatgtt gcaacaatcg gtaataatga
     4501 agggatgacc atttcacacg aagctttaaa tcacctttac gacgaagcaa aatttatagt
     4561 gacatgtagc aatgttatag atgaatcagg tcatttacca aataatatcg tttcttctta
     4621 tattaaggat atggacggtg tgaaaatact attcgttgca gcgacagcac cttttacccc
     4681 attttatcgt gcactagatt ggattgttac cgatccactt gaatctataa aagaagaaat
     4741 taaacatcaa cgaggtaaat ttgatgtaat aatcgtgcta agtcattgtg gcattttctt
     4801 cgatgaaaca ttatgccaag aattgcctga aattgatgtc atttttggta gtcatacgca
     4861 tcattatttt gaacatggtg agatcaataa tggtgtactg atggcggcag ctggaaagta
     4921 tggtaattat cttggagagg ttaatttaac ttttgaggca cataaagtag tacataaaac
     4981 tgcaaagatt attcctttag aaacattacc tgaagttgaa acttcatttg aagaagaagg
     5041 aaaaacgtta atgtccaatt cagtaattca acatccagta gtgcttaagc gtagtatgaa
     5101 tcacataact gaagctgcat acttattagc tcaaagtgtt tgtgagtata cacatgcaca
     5161 atgtgccatc atcaatgctg gcttactcgt taaagatatt gtaaaagatg aagtgacaga
     5221 atatgacatt catcaaatgt taccgcatcc gattaatatg gtaagggtta gactttctgg
     5281 tgtgaaatta aaagagatta taactaaaag taataaacaa gaatatatgt atgaacatgc
     5341 acaaggtttg ggtttcagag ggaatatatt tggaggatat attctttata atttagggta
     5401 cattcattct acagggcgtt actatctgaa tggagaagaa atcgaagacg acaaagaata
     5461 tgtattaggt acgatagaca tgtatacgtt cggtcgttat ttcccaacac tgaaagaatt
     5521 accaaaagag tatttaatgc cagagttttt aagagatata tttaaagaaa aattattgga
     5581 atattaaaaa gtaagattat tggattttca tttgtcatga atttcgatat aatgtttaaa
     5641 gatacactta acaggagggt atgtgttgtt atggcgacaa aaaacgagga aatattacgt
     5701 aaaccggatt ggttgaaaat aaaattaaat accaacgaaa actatacagg acttaagaag
     5761 atgatgaggg aaaaaaatct taatactgta tgtgaagaag ctaaatgtcc taatatacat
     5821 gaatgttggg gtgcacgtcg tacagcgaca tttatgattt taggtgccgt atgtacaaga
     5881 gcttgtcgtt tttgtgcggt taagacaggt ttacctaatg aacttgattt aaatgagcct
     5941 gaacgtgtag ctgaatcagt tgaattaatg aatttgaaac acgttgttat cactgctgtt
     6001 gcgcgtgatg atttaagaga tgctggttca aatgtttatg ctgagacagt acgtaaagtt
     6061 agagaaagaa atccatttac aacgattgaa attttaccat cagatatggg cggggactat
     6121 gatgcgttag aaacattaat ggcgtcaaga cctgacattt taaaccataa tattgaaact
     6181 gttcgtcgct taacaccgag agttcgtgcg cgtgcgactt acgacagaac attagagttt
     6241 ttacgtcgtt caaaagaatt acaaccagat atcccaacta aatcaagtat tatggttgga
     6301 ttaggtgaaa ctatagaaga aatttatgaa acgatggatg atttacgtgc gaatgatgta
     6361 gatattttaa cgattggtca atatttacaa ccttcacgta aacatttaaa ggttcaaaaa
     6421 tattacacgc ctttagagtt tggtaaatta agaaaagtgg caatggataa agggtttaaa
     6481 cattgccaag ctggaccttt agtacgtagt tcttatcatg cggatgagca agtaaatgaa
     6541 gctgctaaag aaaagcaacg ccaaggtgag gcatagttaa atagttaata tttaacaatt
     6601 aataaggcat aaaggcttag tttgtacaaa acgaacgtgt catagaagta atcattattt
     6661 ttatgaaaca ctaaaagtat aaactgagcc tttcattgtc atagataggt gaagaatttg
     6721 ataaaagtag atcaacatta ctttgaatta atagaaaatt atcgcgaatg ttttaatgaa
     6781 gaacaattta ttgctaggta ttcagatatt ttagacaaat atgattacat tgttggtgac
     6841 tatggttacg atcaattacg attaaaaggc ttttacaaag attctaataa aaaagcagag
     6901 atgagtaaac gtttttcaaa tattcaagat tacatatttg aatattgtaa ctttggttgt
     6961 ccttactttg tattaagaca tttgtctaaa caagaggtta aaaagttaat cgaagaagtt
     7021 catccgtctg atgtgataga tgacgacaat aaacttcaag atgtgaagat taagccaacc
     7081 attcaagata ctgaacatta ataaaaccct tagctagatt gaaaatggga atcatgcaat
     7141 tcaagcatgg acctgtaatc tagttagggg tttttatctt taatga
//
LOCUS       DANBWH010000140         7092 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.144, whole genome shotgun sequence.
ACCESSION   DANBWH010000140 DANBWH010000000
VERSION     DANBWH010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7092
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.144"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            142..6957
                     /gene="sasA"
                     /locus_tag="PPY92_001992"
     CDS             142..6957
                     /gene="sasA"
                     /locus_tag="PPY92_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin SasA"
                     /protein_id="HDJ2625979.1"
                     /translation="MSKRQKAFHDSLANEKTRVRLYKSGKNWVKSGIKEIEMFKIMGL
                     PFISHSLVSQDNQSISKKMTGYGLKTTAVIGGAFTVNMLHDQQAFAASDAPLTSELNT
                     QSETVGNQNSTTIEASTSTADSTSVTKNSSSVQTSNSDTVSSEKSEKVTSTTNSTSNQ
                     QEKLTSTSESTSSKNTTSSSDTKSVASTSSTEQPINTSTNQSTASNNTSQSTTPSSVN
                     LNKTSTTSTSTAPVKLRTFSRLAMSTFASAATTTAVTANTITVNKDNLKQYMTTSGNA
                     TYDQSTGIVTLTQDAYSQKGAITLGTRIDSNKSFHFSGKVNLGNKYEGHGNGGDGIGF
                     AFSPGVLGETGLNGAAVGIGGLSNAFGFKLDTYHNTSKPNSAAKANADPSNVAGGGAF
                     GAFVTTDSYGVATTYTSSSTADNAAKLNVQPTNNTFQDFDINYNGDTKVMTVKYAGQT
                     WTRNISDWIAKSGTTNFSLSMTASTGGATNLQQVQFGTFEYTESAVTQVRYVDVTTGK
                     DIIPPKTYSGNVDQVVTIDNQQSALTAKGYNYTSVDSSYASTYNDTNKTVKMTNAGQS
                     VTYYFTDVKAPTVTVGNQTIEVGKTMNPVVLTTTDNGTGTVTNTVTGLPSGLSYDSAT
                     NSIIGTPTKIGQSTVTVVSTDQANNKSTTTFTINVVDTTAPTVTPIGDQSSEVYSPIS
                     PIKIATQDNSGNAVTNTVTGLPSGLTFDSTNNTISGTPTNIGTSTISIVSTDASGNKT
                     TTTFKYEVTRNSMSDSVSTSGSTQQSQSVSTSKADSQSASTSTSGSIVVSTSASTSKS
                     TSVSLSDSVSASKSLSTSESNSVSSSTSTSLVNSQSVSSSMSGSVSKSTSLSDSISNS
                     NSTEKSESLSTSTSDSLRTSTSLSDSLSMSTSGSLSKSQSLSTSISGSSSTSASLSDS
                     TSNAISTSTSLSESASTSDSISISNSIANSQSASTSKSDSQSTSISLSTSDSKSMSTS
                     ESLSDSTSTSGSVSGSLSIAASQSVSTSTSDSMSTSEIVSDSISTSGSLSASDSKSMS
                     VSSSMSTSQSGSTSESLSDSQSTSDSDSKSLSLSTSQSGSTSTSTSTSASVRTSESQS
                     TSGSMSASQSDSMSISTSFSDSTSDSKSASTASSESISQSASTSTSGSVSTSTSLSTS
                     NSERTSTSVSDSTSLSTSESDSISESTSTSDSISEAISASESTSISLSESNSTSDSES
                     QSASAFLSESLSESTSESTSESVSSSTSESTSLSDSTSESGSTSTSLSNSTSGSASIS
                     TSTSISESTSTFKSESVSTSLSMSTSTSLSNSTSLSTSLSDSTSDSKSDSLSTSMSTS
                     DSISTSKSDSISTSTSLSGSTSESESDSTSSSESKSDSTSMSISMSQSTSGSTSTSTS
                     TSLSDSTSTSLSLSASMNQSGVDSNSASQSASNSTSTSTSESDSQSTSTYTSQSTSQS
                     ESTSTSTSLSDSTSISKSTSQSGSTSTSASLSGSESESDSQSISTSASESTSESASTS
                     LSDSTSTSNSGSASTSTSLSNSASASESDSSSTSLSDSTSASMQSSESDSQSTSASLS
                     DSLSTSTSNRMSTIASLSTSVSTSESGSTSESTSESDSTSTSLSDSQSTSRSTSASGS
                     ASTSTSTSDSRSTSASTSTSMRTSTSDSQSMSLSTSTSTSMSDSTSLSDSVSDSTSDS
                     TSASTSGSMSVSISLSDSTSTSTSASEVMSASISDSQSMSESVNDSESVSESNSESDS
                     KSMSGSTSVSDSGSLSVSTSLRKSESVSESSSLSGSQSMSDSVSTSDSSSLSVSTSLR
                     SSESVSESDSLSDSKSTSGSTSTSTSGSLSTSTSLSGSESVSESTSLSDSISMSDSTS
                     TSDSDSLSGSISLSGSTSLSTSDSLSDSKSLSSSQSMSGSESTSTSVSDSQSSSTSNS
                     QFDSMSISASESDSMSTSDSSNISGSNSTSTSLSTSDSMSGSVSVSTSTSLSDSISGS
                     TSVSDSSSTSTSTSLSDSMSQSQSTSTSASGSLSTSISTSMSMSASTSSSQSTSVSTS
                     LSTSDSISDSTSISISGSQSTVESESTSDSTSISDSESLSTSDSDSTSTSTSDSTSGS
                     TSTSISESLSTSGSGSTSVSDSTSMSESDSTSVSMSQDKSDSTSISDSESVSTSTSTS
                     LSTSDSTSTSESLSTSMSGSQSISDSTSTSMSGSTSTSESNSMHPSDSMSMHHTHSTS
                     TSRLSSEATTSTSESQSTLSATSEVTKHNGTPAQSEKRLPDTGDSIKQNGLLGGVMTL
                     LVGLGLMKRKKKKDENDQDDSQA"
BASE COUNT         2576 a         1386 c         1306 g         1824 t
ORIGIN      
        1 attccatcta gacgaataaa aatgaatttt tacaaatttt aacaaaatga gtatttttgt
       61 atcaaaaaag agttgtatta aattgaatag tggcatatat tataatctaa tgaatgtaaa
      121 gataatacaa ggagttatta catgagtaaa agacagaaag catttcatga cagcttagca
      181 aacgaaaaaa caagagtaag actttataaa tctggaaaaa attgggtaaa atccggaatt
      241 aaagaaatag aaatgttcaa aattatgggg ctaccattta ttagtcatag tttagtgagt
      301 caagataatc aaagcattag taaaaaaatg acgggatacg gactgaaaac tacggcggtt
      361 attggtggtg cattcacggt aaatatgttg catgaccagc aagcttttgc ggcttctgat
      421 gcaccattaa cttctgaatt aaacacacaa agtgaaacag taggtaatca aaactcaacg
      481 acaatcgaag catcaacatc aacagccgat tccacaagtg taacgaaaaa tagtagttcg
      541 gtacaaacat caaatagtga cacagtctca agtgaaaagt ctgaaaaggt cacttcgaca
      601 actaatagta caagcaatca acaagagaaa ttgacatcta catcagaatc aacatcctca
      661 aagaatacta catcaagttc tgatactaaa tctgtagctt caacttcaag tacagaacaa
      721 ccaattaata catcaacaaa tcaaagtact gcatcaaata acacttcaca aagcacaacg
      781 ccatcttcgg tcaacttaaa caaaactagc acaacgtcaa ctagcaccgc accagtaaaa
      841 cttcgaactt tcagtcgctt agctatgtca acatttgcgt cagcagcgac gacaaccgca
      901 gtaactgcta atacaattac agttaataaa gataacttaa aacaatatat gacaacgtca
      961 ggtaatgcta cctatgatca aagtaccggt attgtgacgt taacacagga tgcatacagc
     1021 caaaaaggtg ctattacatt aggaacacgt attgactcta ataagagttt tcatttttct
     1081 ggaaaagtaa atttaggtaa caaatatgaa gggcatggaa atggtggaga tggtatcggt
     1141 tttgcctttt caccaggtgt attaggtgaa acagggttaa acggtgccgc agtaggtatt
     1201 ggtggcttaa gtaacgcatt tggcttcaaa ttggatacgt atcacaatac atctaaacca
     1261 aattcagctg caaaggcgaa tgctgaccca tctaatgtag ctggtggagg tgcgtttggt
     1321 gcatttgtaa caacagatag ttatggtgtt gcaacaacgt atacatcaag ttcaacagct
     1381 gataatgctg cgaagttaaa tgttcaacct acaaataaca cgttccaaga ttttgatatt
     1441 aactataatg gtgatacaaa ggttatgact gtcaaatatg caggtcaaac atggacacgt
     1501 aatatttcag attggattgc gaaaagtggt acgaccaact tttcattatc aatgacagcc
     1561 tcaacaggtg gcgcgacaaa tttacaacaa gtacaatttg gaacattcga atatacagag
     1621 tctgctgtta cacaagtgag atacgttgat gtaacaacag gtaaagatat tattccacca
     1681 aaaacatatt caggaaatgt tgatcaagtc gtgacaatcg ataatcagca atctgcattg
     1741 actgctaaag gatataacta cacgtccgtc gatagttcat atgcgtcaac ttataatgat
     1801 acaaataaaa ctgtaaaaat gacgaatgct ggacaatcag tgacatatta ttttactgat
     1861 gtaaaagcac caactgtaac tgtaggcaat caaaccatag aagtgggtaa aacaatgaat
     1921 cctgttgtgt tgactacaac ggataatggt actgggactg tgacaaatac agttacagga
     1981 ttaccaagcg gattaagtta cgatagtgca acgaattcaa tcattgggac accaacaaaa
     2041 attggtcaat caacagtgac agttgtgtct actgaccaag caaataacaa atcgacgaca
     2101 acttttacaa taaatgttgt ggatacgaca gcaccaacag tgacaccaat aggagatcaa
     2161 tcatcagaag tgtattcacc aatatccccg attaaaattg ctacgcaaga taacagtgga
     2221 aatgcggtga cgaatacagt gactggattg ccatccggac taacatttga tagtacaaat
     2281 aatactatta gtggtacacc aacaaacatt ggtacaagta ctatatcaat cgtttctaca
     2341 gatgcgagcg gtaacaaaac gacgacaact tttaaatatg aagtaacaag aaatagcatg
     2401 agtgattccg tatcaacatc aggaagtaca caacaatctc aaagtgtctc aacaagtaaa
     2461 gctgactcac aaagtgcatc aacgagtaca tcaggatcga ttgtggtatc tacatcagct
     2521 agtacctcga aatcgacaag tgtaagccta tctgattctg tgagtgcatc taagtcatta
     2581 agcacatctg aaagtaatag tgtatcaagc tcaacaagca caagtttagt gaattcacaa
     2641 agtgtatcat caagcatgtc aggttcagtt agtaaatcaa catcattaag cgattcgatt
     2701 tcgaactcta acagtactga aaaatccgaa agtctatcaa caagtacatc tgattcattg
     2761 cgtacatcaa catcactcag tgactcatta agtatgagta catcaggaag cttgtctaag
     2821 tcacaaagct tatcaacgag tatatcaggg tcgtctagta catcagcatc attaagtgac
     2881 agtacatcga atgcaattag tacatcaaca tcattgagcg agtcagctag cacctcggac
     2941 tctatcagta tttcaaatag catagccaac tctcaaagtg cgtcaacaag caaatcagat
     3001 tcacaaagta catcaatatc attaagtaca agtgattcaa aatcgatgag tacatcagaa
     3061 tcattgagcg attcgacgag cacaagtggt tctgtttctg gatcactaag catagcagca
     3121 tcacaaagtg tctcaacaag tacatcagac tcgatgagta cttcagagat agtaagtgac
     3181 tctatcagta caagtgggtc attatctgca tcagacagta aatcaatgtc tgtaagtagt
     3241 tcaatgagca cgtctcagtc aggtagtaca tcagaatcat taagtgattc acaaagtaca
     3301 tctgattctg atagtaagtc attatcacta agtactagtc aatcaggttc aacaagtaca
     3361 tcaacgtcga caagtgcttc agtacgtact tcggaatcgc aaagtacgtc tggttcaatg
     3421 agtgcaagtc aatccgattc aatgagcata tcaacgtcgt ttagtgattc aacgagtgat
     3481 agcaaatcag catcaactgc atcaagtgaa tcaatatcac aaagtgcttc tacgagcaca
     3541 tctggttcgg taagtacttc gacatcgtta agtacaagta attcagaacg tacatcaaca
     3601 tctgtgagtg attccacaag cttaagtaca tcagagtctg attcaataag tgaatcaacg
     3661 tcaacgagcg attctataag tgaagcaata tctgcttcag agagcacgtc tatatcatta
     3721 agtgaatcaa atagtactag cgattcagaa tcacaaagtg catctgcctt tttaagtgaa
     3781 tcattaagtg aaagtacgtc tgaatcaaca tcagagtcag tgagtagttc gacaagtgag
     3841 agtacgtcat tatcagacag tacatcagaa tctggtagca catcaacatc attaagtaat
     3901 tcaacaagtg gtagtgcgtc catttcaaca tcgacaagta tcagtgaatc aacgtcaacg
     3961 tttaagagcg agagtgtttc aacatcactg agtatgtcaa cgagtacaag tttgtctaac
     4021 tctacaagtt tgtcaacatc attaagtgat tccacaagtg atagtaagtc tgattcatta
     4081 agtacatcaa tgtcgacaag tgattcaatc agtacaagta aatctgattc cattagtaca
     4141 tctacatcat taagtggttc tacaagtgaa agtgaatccg actcaacatc atcaagtgaa
     4201 agtaaatccg attcaacatc aatgagcata agtatgtctc aatcaacatc aggaagtaca
     4261 agtacgtcaa cgagtacaag tttgtctgac tcaacgagta cgtcattgtc actaagtgca
     4321 tcaatgaatc aaagcggagt agactcaaac tcagcaagcc aaagtgcctc aaactcaaca
     4381 agtacaagca cgagcgaatc cgattcacaa agcacatcaa catatacaag tcagtcaaca
     4441 agccaaagtg aatcaacatc gacatcaacg tcactaagcg attcaacaag tatatctaaa
     4501 agtacgagcc aatcaggttc aacaagcaca tcagcatcat taagtggttc agagagtgaa
     4561 tctgattcac aaagtatctc aacaagtgca agtgaatcaa catcagaaag tgcgtcaaca
     4621 tcactcagtg actcaacaag tacaagtaac tcaggatcag caagtacgtc aacatcgctc
     4681 agtaactcag caagcgcaag tgaatccgat tcgtcgtcaa catctttaag tgattcaaca
     4741 tctgcgtcaa tgcaaagcag tgaatccgat tcacaaagca catcagcatc attaagtgat
     4801 tcgctaagta catcaacttc aaaccgcatg tcgaccattg caagtttatc aacatcggta
     4861 agtacatcag aatctggctc aacatcagaa agtacaagtg aatccgattc aacatcaaca
     4921 tcattaagcg attcacaaag cacatcaaga agtacaagtg catcaggatc agcaagtaca
     4981 tcaacatcaa caagtgactc tcgtagtaca tcagcttcaa ctagtacttc gatgcgtaca
     5041 agtactagtg attcacaaag tatgtcgctt tcaacaagta catcaacaag tatgagtgat
     5101 tcaacgtcat tatctgatag tgtgagtgat tcaacatcag actcaacaag tgcgagtaca
     5161 tctggttcga tgagtgtatc tatatcgtta agtgattcga caagtacatc aacatcggct
     5221 agtgaagtaa tgagcgcaag catatctgat tcacaaagta tgtcagaatc tgtaaatgat
     5281 tcagaaagtg taagtgaatc taattctgaa agtgactcta aatcgatgag tggctcaaca
     5341 agtgtcagtg attctggctc attgagcgtc tcaacgtcat taagaaaatc agaaagtgta
     5401 agcgagtcaa gttcattgag tggctcacaa tcgatgagcg attcagtaag cacaagcgat
     5461 tcgtcatcat taagtgtatc gacgtcacta agaagttcag aaagcgtgag tgaatctgat
     5521 tcattaagtg attcaaaatc aacaagtggt tcgacttcaa caagtacatc tggttcattg
     5581 agtacctcaa catcattaag tggttcagaa agcgtaagcg agtctacctc gctaagtgat
     5641 tcaatatcaa tgagtgattc tactagtaca agtgactccg actcattaag tggatcaata
     5701 tctttaagtg gttccacaag tcttagcact tcggattcat taagtgattc aaaatcattg
     5761 agtagctcgc aaagtatgag tggatcagaa tcaacgtcaa caagtgtgag cgattcgcag
     5821 tcaagctcaa caagtaatag tcaatttgac tctatgagca tcagtgcatc agaaagcgac
     5881 tcaatgtcta caagtgattc gtctaacatc agtggatcaa attcaacgag tacatcactt
     5941 tcaacatctg actcaatgag cggaagcgta tcagtttcaa catcgacaag tttaagtgac
     6001 tcaatatcag gttcaacaag tgtaagtgac tcgagctcaa caagcacatc tacatcatta
     6061 agtgattcaa tgtcacaaag ccagtcaaca agtacaagtg catctggttc cttaagtaca
     6121 tcgatatcaa catcaatgtc aatgagtgct agtacatcgt catcacaaag cacatcggtg
     6181 tcgacatcat tatcaacatc agacagtatc agtgattcta cttcaataag tatcagtggt
     6241 tcacaaagta cagtagaatc agaatctaca agtgattcaa cttctatcag tgactcagaa
     6301 tcattgagta catcagattc agactcgaca tcgacaagta catcggactc aacaagtggt
     6361 tcaacttcaa caagcatatc tgaatcatta agtacgtctg gttcaggttc aacgagcgta
     6421 tctgactcaa catcaatgag tgaatccgat tcaacaagtg tttcaatgtc acaagacaaa
     6481 tctgactcaa catcaattag cgactcagaa tcagtgtcaa caagcacatc aacgtcattg
     6541 agcacatccg attcgacaag cacatccgaa tcgctgagta catctatgtc tggttcacaa
     6601 agcatttctg actcaacatc aacaagtatg tccggctcaa caagtacatc tgaatctaac
     6661 tcaatgcatc cgtcagactc aatgagtatg catcatactc acagcacgag cacatctcgc
     6721 ttatcaagtg aagcaacaac gagcacgagt gaatctcagt ctacattaag tgcaacatct
     6781 gaagtgacta aacataatgg cacgcctgca caaagtgaaa aaagattgcc agatacaggt
     6841 gactcaataa aacaaaatgg attactaggt ggcgttatga cattattagt tggtttaggt
     6901 ttaatgaaga gaaagaaaaa gaaagatgaa aatgatcaag atgattctca agcataaatt
     6961 gaatttatag ctggctaaaa ttaaaaagac tgaggcacca atcactagaa aaatcgatat
     7021 aggattttaa taattagtga tgaaccctta gtctttttaa tgtttttgat ataaaagagg
     7081 tgcaacatgt ta
//
LOCUS       DANBWH010000141         7055 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.145, whole genome shotgun sequence.
ACCESSION   DANBWH010000141 DANBWH010000000
VERSION     DANBWH010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7055
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.145"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            82..399
                     /locus_tag="PPY92_001993"
     CDS             82..399
                     /locus_tag="PPY92_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HDJ2625980.1"
                     /translation="MNKLLQSLSALGVSATLVTPNLNADATTNTTPQIKGANDIVIKK
                     GQDYNLLNGISAFDKEDGDLTDKIKVDGQIDTSKSGKYQIKYHVTDSDGAIKISTRYI
                     EVK"
     gene            complement(540..665)
                     /locus_tag="PPY92_001994"
     CDS             complement(540..665)
                     /locus_tag="PPY92_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625981.1"
                     /translation="MERKRKHFKKEFAKSNLSLNNEKWKFVCSKSKHFLKRAVKF"
     gene            970..2070
                     /gene="qoxA"
                     /locus_tag="PPY92_001995"
     CDS             970..2070
                     /gene="qoxA"
                     /locus_tag="PPY92_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit II"
                     /protein_id="HDJ2625982.1"
                     /translation="MSKFKSLLLLFGTLILLSGCSNIEIFNAKGPVASSQKFLILYSI
                     VFMLVICFVVLGMFAIFIYKYSYNKNAESGKMHHNAIIETIWFVIPIIIVAALAIPTV
                     KTLYDYEKPPKSEKDPMVVYAVSAGYKWFFAYPDEHIETVNTLTIPKDRPVVFKLQAM
                     DTMTSFWIPQLGGQKYAMTGMTMNWTLEASQTGTFRGRNSNFNGEGFSRQTFKVNAVS
                     QKDYDKWVKEVKGKKTLDQDTFDKQLLPSTPNKALEFNGTHMAFVDPAADPEYIFYAY
                     KRFNFELKDPNFTSEENMFKDVSDKPLIPARKAQITNANYKRHGMKLMILGNDEPYNN
                     EFKKDESKNAKEMKKISKDAQDQDNDDHGGGH"
     gene            2070..4058
                     /gene="qoxB"
                     /locus_tag="PPY92_001996"
     CDS             2070..4058
                     /gene="qoxB"
                     /locus_tag="PPY92_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit I"
                     /protein_id="HDJ2625983.1"
                     /translation="MNFPWDQLLVKGNWMITMAQIGAPFLVIGLIAVITYFKLWKYLY
                     KEWFTSVDHKKIGIMYLICAVLMFVRGGIDALLIRAQLTVPDNKFLESNHYNEIFSTH
                     GVIMIIFMAMPFIFGLWNIVVPLQIGARDVAFPVLNNVSFWLFFAGMILFNLSFIIGG
                     SPAAGWTNYAPLAGEFSPGPGVNYYLIAIQISGLGTLATGINFFVTILRCKTPTMKFM
                     QMPMFTVTTFITTLIVILAFPPLTVALALMTTDRIFDTAFFTVAHGGMPMLWANFFWV
                     WGHPEVYIVILPAFGIYSEIIPTFARKRLFGHQSMVWATAGIAFLSFLVWVHHFFTMG
                     NGALINSFFSISTMLIGIPTGVKLFNWLLTLYKGRITFESPMLFSLAFIPNFLLGGVT
                     GVMLAMASADYQYHNTYFLVAHFHYTLVTGVVFACLAGLIFWYPKMMGYKLNETLNKW
                     CFWFFMIGFNVCFLPQFILGLDGMPRRLYTYMPSDGWFLLNLISTIGALLMAIGFLFL
                     VVSIVYSHFKSPREATGDNWDGLGRTLEWTTASAIPPKYNFAITPDWNDYDTFVDMKE
                     HGRHYLDNHNYKDIHMPNNTPVGFWIGIFMTIGGFFLIFETVIPALICLFGIFGTMIY
                     RSFQIDHGYHIPAAEVAETEARLREARIKEREAVSHES"
     gene            4048..4653
                     /gene="qoxC"
                     /locus_tag="PPY92_001997"
     CDS             4048..4653
                     /gene="qoxC"
                     /locus_tag="PPY92_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit III"
                     /protein_id="HDJ2625984.1"
                     /translation="MSHDTNTIDSRTHEGELNKLGFWIFITAEFALFGTLFATLLTLQ
                     HGGDYAGKMTTELFELPLVLIMTFALLFSSYTCGIAIYYMRQEKQKLMMFWMIITLLL
                     GLVFVGFEIYEFAHYASEGVNPTIGSYWSSFFILLGTHGCHVSLGIVWAICLLIQIQR
                     RGLDKYNAPKLFIVSLYWHFLDVVWVFIFTAVYMIGMVYSG"
     gene            4662..4940
                     /gene="qoxD"
                     /locus_tag="PPY92_001998"
     CDS             4662..4940
                     /gene="qoxD"
                     /locus_tag="PPY92_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit IV"
                     /protein_id="HDJ2625985.1"
                     /translation="MKHTVGFIASIVLTLLAVYVTLYTSLTFHAKLTIIFGFAFVQAG
                     LQLLMFMHLTEGKDGRLQTFKVIFALVITLCFVVGTYWVMQGGHSSHL"
     gene            complement(5477..6670)
                     /gene="fmtA"
                     /locus_tag="PPY92_001999"
     CDS             complement(5477..6670)
                     /gene="fmtA"
                     /locus_tag="PPY92_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala esterase FmtA"
                     /protein_id="HDJ2625986.1"
                     /translation="MKFNKVKLVIHACVLLFIIISIALIFHRLQTKTHSIDPIHKETK
                     LSDNEKYLVDRNKEKVAPSKLKEVYNSKDPKYKKIDKYLQSSLFNGSVAIYENGKLKM
                     SKGYGYQDFEKGIKNTPNTMFLIGSAQKFSTGLLLKQLEEEHKININDPVSKYLPWFK
                     TSKPIPLKDLMLHQSGLYKYKSSKDYKNLDQAVKAIQKRGIDPKKYKKHMYNDGNYLV
                     LAKVIEEVTGKSYAENYYTKIGDPLKLQHTAFYDEQPFKKYLAKGYAYNSTGLSFLRP
                     NILDQYYGAGNLYMTPTDMGKLITQIQQYKLFSPKITNPLLHEFGTKQYPDEYRYGFY
                     AKPTLNRLNGGFFGQVFTVYYNDKYVVVLALNVKGNNEVRIKHIYNDILKQNKPYNTK
                     GVIVQ"
BASE COUNT         2253 a         1190 c         1087 g         2525 t
ORIGIN      
        1 ctatagtatg atgtgtaaat aatataaatt aaggtatagt aaagctaact cagaaatgac
       61 ttatcattcg gaggttacat tatgaataaa ctattacagt cattatcagc cctcggtgtt
      121 tctgctacac tagtaacacc aaatttaaat gcagatgcaa cgacgaatac tacaccacaa
      181 attaaaggcg ctaatgatat cgttattaag aaaggtcaag attataacct tctaaacggc
      241 ataagtgcat ttgataaaga agatggagat ttaaccgata aaattaaagt cgatggccaa
      301 attgatacat ctaaatctgg taaatatcaa attaaatatc atgtcactga ttcagatggt
      361 gcaattaaaa tttccactag gtatattgag gttaaatagc cctcatcact atactgtaaa
      421 taaaatggta gcaaacgaac atgttttgct accattttat ttgttattcc aacttcatct
      481 gcaactttaa cctaaatatt gtattttttc tgtataccaa aggactacct atcaaattat
      541 taaaacttaa ctgctctttt taaaaaatgt tttgattttg aacaaacaaa tttccacttt
      601 tcattgttta acgataaatt acttttggca aattcctttt taaagtgttt gcgcttcctt
      661 tcaatgaact agccatcatt ttcaatttat tagacaattt caaacttttt ttattttcat
      721 tcaattaacc tttaattgaa agctattctc aactttcctt ttaaatatga agcaattttt
      781 tcaaaaacgc tattagtcac aaaatgtaca cataatttta acaaatgtgt gaacccacgt
      841 cacaccttga tttatcaaca tttttgatac aattttatta atttttttcc cataagccct
      901 tgtaaatatt gtgtaataac ttaaaattaa tgttaagccc tacatttgta gtattaggag
      961 gtcaaaaaag tgtcaaaatt taagtctttg cttctattat ttggcacact aattttactt
     1021 agtggttgtt caaatataga aattttcaac gcaaaagggc cagtagcaag tagtcagaag
     1081 ttcttgattc tttattcaat cgtcttcatg cttgttattt gtttcgttgt acttggcatg
     1141 ttcgccattt ttatttacaa gtatagttat aataagaatg ccgaatctgg taagatgcac
     1201 cataatgcca tcattgaaac aatatggttt gtaataccta tcataatcgt tgctgcttta
     1261 gctattccta cagttaaaac tttatacgat tacgaaaaac caccgaagag tgagaaagat
     1321 ccaatggttg tatatgcagt aagtgcagga tacaaatggt tctttgctta tccagatgaa
     1381 catatagaga ctgttaatac attaacaatc cctaaagatc gtcctgtcgt atttaagctt
     1441 caagctatgg atacaatgac aagtttctgg attccacaat taggtggtca aaaatatgcc
     1501 atgactggca tgacaatgaa ttggacgtta gaagcatctc aaactggtac gttcagaggt
     1561 cgtaactcta acttcaatgg tgaaggattc tcacgtcaaa catttaaagt aaatgccgtt
     1621 agtcaaaaag actacgacaa atgggtgaaa gaagttaaag gtaagaaaac gttagatcaa
     1681 gatacatttg ataaacaatt attaccaagc acacctaata aagctttaga atttaatggt
     1741 acacatatgg cgtttgttga tccagcagcc gatccagaat acatcttcta tgcatataaa
     1801 cgtttcaatt tcgaattgaa agatccaaac ttcactagtg aagaaaatat gtttaaagat
     1861 gtatcagaca aaccattaat acctgctcgt aaagctcaaa ttacaaatgc gaactataaa
     1921 cgacatggta tgaagttgat gattcttgga aatgacgaac catataacaa tgagttcaag
     1981 aaagatgaat cgaaaaatgc gaaagaaatg aagaaaattt caaaagatgc gcaagatcaa
     2041 gacaatgatg atcatggagg tggacattaa tgaattttcc atgggatcaa ttactagtta
     2101 aaggtaactg gatgattaca atggcacaaa ttggtgcccc attcttagtt attggtttaa
     2161 tcgcagtaat tacttacttt aaattatgga aatatcttta caaagaatgg ttcacatctg
     2221 tagaccataa gaaaatcggt attatgtatt taatctgtgc cgtattaatg ttcgtccgtg
     2281 gtggtattga tgcgttactt attcgtgcgc aattaacagt acctgataac aaatttttag
     2341 aatctaacca ctataacgaa atttttagta cacatggtgt aatcatgatt atcttcatgg
     2401 caatgccatt tatctttggt ttatggaata tcgttgttcc attacaaatt ggagcacgtg
     2461 atgttgcatt cccagtatta aacaacgtaa gtttctggct attctttgca ggtatgattt
     2521 tattcaacct gtcatttatt attggtggtt caccagcagc tggttggaca aactatgcgc
     2581 cactagcagg tgaattcagt cctggtccgg gtgttaacta ttacttaata gctatacaaa
     2641 tttcagggct tggtacctta gctacaggta tcaacttctt tgtaacaatt ttaagatgta
     2701 aaacaccaac tatgaagttt atgcaaatgc caatgtttac agtaacaaca tttattacta
     2761 cattaattgt tattcttgct ttccctcctt taacagtagc gttagcatta atgacaactg
     2821 atagaatatt tgacacagca ttcttcacag ttgcacatgg cggtatgcca atgctttggg
     2881 ctaacttctt ctgggtatgg gggcaccctg aagtttatat cgttatcctt ccagcatttg
     2941 gtatttactc agaaattatc ccgacattcg ctcgtaagcg tttattcgga catcaaagta
     3001 tggtatgggc aactgccggt atcgcgttcc ttagtttctt agtttgggtt caccatttct
     3061 tcacaatggg taatggtgcg ttaatcaact cattcttctc aatttcaaca atgctgattg
     3121 gtattccaac tggtgtgaaa ttgtttaact ggttattaac attatataaa ggacgtatta
     3181 catttgagtc accaatgcta ttctcattag catttatccc taacttcctt ttaggtggtg
     3241 ttacgggtgt aatgttagca atggcatcag ctgactatca atatcacaat acgtacttct
     3301 tagtagctca cttccactat acattggtta ctggtgttgt atttgcctgc ttagctggtt
     3361 taatcttctg gtatccaaag atgatgggtt acaaattaaa cgaaacatta aacaaatggt
     3421 gcttctggtt cttcatgatc ggatttaacg tttgtttctt accacaattc attcttggat
     3481 tagatggtat gccacgtcgt ttatacactt atatgccatc agatggttgg ttcttattaa
     3541 acttaatttc aactatcggt gcattattaa tggcaatcgg attcttattc cttgttgtta
     3601 gtatcgtata cagtcacttc aaatctccac gtgaagcaac tggagataac tgggatggcc
     3661 ttggccgtac tctagagtgg actactgcat cagcaattcc acctaaatac aactttgcca
     3721 ttacaccaga ttggaatgac tacgacacat ttgttgatat gaaagaacat ggtcgtcatt
     3781 acttagacaa ccataattac aaagacattc atatgccaaa caatactcct gttggattct
     3841 ggattggtat ctttatgact atcggcggtt tcttcttaat tttcgaaact gtaattccag
     3901 cattaatttg tttattcggt atttttggta ctatgattta tcgcagtttc caaatcgatc
     3961 acggatatca tatccctgct gctgaagttg ctgaaactga agctagatta agagaagcgc
     4021 gtattaaaga aagggaggct gtaagtcatg agtcatgata caaacactat tgattcacgc
     4081 acacatgaag gcgaattaaa taagcttggc ttttggattt tcattacagc cgaatttgcg
     4141 ttattcggta ccctatttgc gacactatta actttgcaac atggtggcga ttatgcaggt
     4201 aaaatgacta ctgagttatt tgaattgcct cttgttttaa taatgacgtt tgcattatta
     4261 ttcagttctt acacatgtgg tattgctatt tactacatgc gccaagaaaa acaaaagtta
     4321 atgatgtttt ggatgatcat tacgttactt ttaggtttag tctttgttgg attcgaaatt
     4381 tatgaattcg cacactatgc atcagaaggc gttaacccaa caattggttc ttactggtct
     4441 agtttcttta tcctattagg aacgcatggt tgtcacgtat cgctaggtat cgtttgggcc
     4501 atttgtttat taatccaaat ccaacgacgc ggattagaca aatataatgc gccaaaatta
     4561 tttatagtaa gtttatactg gcacttctta gatgttgttt gggttttcat ctttactgcc
     4621 gtatatatga tagggatggt gtatagcgga tgagtacaat aatgaaacat actgtaggat
     4681 ttatcgcatc tatcgtatta acgcttttag cagtttacgt aacactatac acgtcattaa
     4741 cattccacgc gaagttgaca attatctttg gctttgcatt cgtccaagca ggacttcaat
     4801 tattaatgtt catgcattta actgaaggta aagatggacg tttacaaaca ttcaaagtta
     4861 tctttgctct tgtaattaca ctttgtttcg ttgtcggaac atattgggtt atgcaaggcg
     4921 gtcactcttc acacttataa acaacatttt aataatgtaa agtttaacca gctaacactt
     4981 ttgttagctg gtttttattt tccttcaatt tttaaatggt taagtcccct tctatatctt
     5041 ataagacaat cattataatc aattcaatta atacattaac aaccacaact aataaatata
     5101 gtaacttcaa aatccatatt tatgtctaaa gataatctca atgttgttca cgtcaataaa
     5161 attatcccta ggattttaaa aattgtacat gtttaaacaa tcaaaagtgt acattattaa
     5221 attatcattt ccagttagat ttagaaaaca ttcacaccac gcatggacca acgtattcgt
     5281 cttcattcat tttagtctga tgccaactca ccaaatgatt tcccctttta tcagttaaca
     5341 catattttta aactcatcct tcttcaacct tatactttaa actttttacc actataaaaa
     5401 aaaagatata actcgtatac tggtcagata aggtcgatgc cttaatgcca tatacatgtt
     5461 atatcttcta tattaattat tgaacaataa cacccttcgt attgtaaggt ttattttgtt
     5521 ttaaaatatc attataaata tgtttgattc gaacttcatt gtttcctttt acatttaatg
     5581 caagtacaac tacatactta tcattatagt aaacagtaaa gacttgtcca aagaatcccc
     5641 cgttaagtct attcaatgtt ggcttagcat agaaaccata tcgatattca tctggatact
     5701 gtttcgtacc aaactcatgt aataatggat tggttatttt aggactgaat aatttatatt
     5761 gttgtatttg agtaattaat ttacccatat ctgttggtgt catatataaa ttacctgcac
     5821 catagtattg gtccaaaata ttaggtctta agaatgaaag tcctgtacta ttataagcat
     5881 aaccttttgc tagatatttt ttaaaaggtt gttcatcata aaatgctgtg tgctgaagtt
     5941 ttaaaggatc tcctattttt gtataataat tctcagcata agatttacct gtaacttctt
     6001 caataacttt cgcaagtact aaataattcc catcgttata catatgcttt ttgtatttct
     6061 taggatcaat accacgtttt tgaatcgctt taactgcttg atctaaattt ttataatctt
     6121 ttgaggattt atatttatat aatccacttt gatgcaacat taaatctttc aatgggatag
     6181 gcttagatgt tttaaaccat ggaaggtatt tacttactgg atcattgata tttattttat
     6241 gttcttcttc taactgtttt aacagtaacc ctgttgaaaa tttttgagct gaacctatta
     6301 aaaacatcgt attcggtgtg tttttaatac ctttttcaaa atcttgatat ccataacctt
     6361 tactcatttt caatttgcca ttttcatata tagctactga accgttaaat aatgaacttt
     6421 gtaaatactt gtcaattttc ttatatttag gatccttgct attatatacc tcttttagtt
     6481 tagacggcgc aaccttttcc ttattacgat ccactaaata tttttcattg tctgataatt
     6541 ttgtttcctt atgtattggg tctatagaat gtgtcttcgt ctgtaatcga tgaaaaatta
     6601 acgctataga aataatgata aataatagta cacacgcatg tataactagt tttactttat
     6661 taaatttcat ttgataattg cctcacaatg tattcatttt atatatttaa atcgttaaaa
     6721 atttgaacct agatgcctaa taatacaact tacaaaaatt aacttatcat tcatttatgc
     6781 tttacgctac atagtaataa gtttttctaa tatgttatct atacaacaaa attaattata
     6841 ccttatcact ttgtataaat cactcgaatt tatattacgc ctttaaattt tttaagacat
     6901 catactaaag tcgcattata tttttaaaaa tgctttttga ttttaacaaa acattcaaat
     6961 tacaaggaac tttgacataa catttgtatt ttttactata aagtactaca atttaaggct
     7021 ataatgtatt agtaatgaag ttgtataaat atcgt
//
LOCUS       DANBWH010000142         7018 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.146, whole genome shotgun sequence.
ACCESSION   DANBWH010000142 DANBWH010000000
VERSION     DANBWH010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7018
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.146"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            195..1691
                     /gene="mqo"
                     /locus_tag="PPY92_002000"
     CDS             195..1691
                     /gene="mqo"
                     /locus_tag="PPY92_002000"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HDJ2625987.1"
                     /translation="MAKSNSKDIVLIGAGVLSTTFGSMLKEIEPDWNIHVYERLDRPA
                     IESSNERNNAGTGHAALCELNYTVLQPDGSIDIEKAKVINEEFEISKQFWGHLVKSGS
                     IENPREFINPLPHISYVRGKNNVKFLKDRYEAMKAFPMFDNIEYTEDIEVMKKWIPLM
                     MKGREDNPGIMAASKIDEGTDVNFGELTRKMAKSIEAHPNATVQFNHEVVDFEQLSNG
                     QWEVTVKNRLTGEKFKQVTDYVFIGAGGGAIPLLQKTGIPESKHLGGFPISGQFLACT
                     NPQVIEQHDAKVYGKEPPGTPPMTVPHLDTRYIDGQRTLLFGPFANVGPKFLKNGSNL
                     DLFKSVKTYNITTLLAAAVKNLPLIKYSFDQVIMTKEGCMNHLRTFYPEARNEDWQLY
                     TAGKRVQVIKDTPEHGKGFIQFGTEVVNSQDHTVIALLGESPGASTSVSVALEVLERN
                     FPEYKTEWAPKIKKMIPSYGESLIEDEKLMRKIRKQTSKDLELGYYEN"
     gene            complement(1885..2775)
                     /locus_tag="PPY92_002001"
     CDS             complement(1885..2775)
                     /locus_tag="PPY92_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002478556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HDJ2625988.1"
                     /translation="MNKEQLEKMKNGKGFIAALDQSGGSTPKALKEYGVNEDQYSNED
                     EMFQLVHDMRTRVVTSPSFSPDKILGAILFEQTMDREVEGKYTADYLADKGVVPFLKV
                     DKGLAEEQNGVQLMKPIDNLDSLLDRANERHIFGTKMRSNILELNEQGIKDVVEQQFE
                     VAKQIIAKGLVPIIEPEVNINAKDKAEIEKVLKAELKKGLDSLNADQLVMLKLTIPTE
                     PNLYKELAEHPNVVRVVVLSGGYSREKANELLKDNDELIASFSRALASDLRADQSKEE
                     FDKALGDAVESIYDASVNKN"
     gene            complement(2898..3314)
                     /locus_tag="PPY92_002002"
     CDS             complement(2898..3314)
                     /locus_tag="PPY92_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625989.1"
                     /translation="MRLKVLFHFIAAIFISFMLLWMTMLFDLISNQSHLKALLLNLDF
                     LIPSDNTPYILEIICHLLIGSVIYFVFVLLFHTSKRLYYLCYIPLFFLFIALYPFLVF
                     IAQRPIFQFSVTELIGWIITHIFFMSLMALVIPRIK"
     gene            3572..4909
                     /locus_tag="PPY92_002003"
     CDS             3572..4909
                     /locus_tag="PPY92_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HDJ2625990.1"
                     /translation="MSKAHQLIQEDEHYFAKSGRIKYYPLVIDHGYGATLVDIEGKTY
                     IDLLSSASSQNVGHAPREVTEAIKAQVDKFIHYTPAYMYHEPLVRLAKKLCELAPGDF
                     EKRVTFGLTGSDANDGIIKFARAYTGRPYIISFTNAYHGSTFGSLSMSAISLNMRKHY
                     GPLLNGFYHIPFPDKYRGMYEQPQANSVEEYLAPLKEMFAKYVPADEVACIVIETIQG
                     DGGLLEPVPGYFEALEKICREHGILIAVDDIQQGFGRTGTWSSVSHFNFTPDLITFGK
                     SLAGGMPMSAIVGRKEIMNCLEAPAHLFTTGANPVSCEAALATIQMIEDQSLLQASAE
                     KGEYVRKRMDQWVSKYNSVGDVRGKGLSIGIDIVSDKKLKTRDASAALKICNYCFEHG
                     VVIIAVAGNVLRFQPPLVITYEQLDTALNTIEDALTALEAGNLDQYDISGQGW"
     gene            complement(4990..6438)
                     /locus_tag="PPY92_002004"
     CDS             complement(4990..6438)
                     /locus_tag="PPY92_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDJ2625991.1"
                     /translation="MGSFFNKIARKEDPAIYQNKDGHLKRTLRVRDFLALGVGTIVST
                     SIFTLPGIVAAEHAGPAVALSFLLAAIVAGLVAFTYAEMAAAMPFAGSAYSWVNVLFG
                     EFFGWVAGWALLAEYFIAVAFVASGFSANLRGLVKPIGIELPAALSNPFGTNGGFIDI
                     IAAIVILLTALLLSRGMSEAARMENILVILKVLAIILFVIVGLTAINVSNYVPFIPEH
                     KVTATGDFGGWQGIYAGVSMIFLAYIGFDSIAANSAEALDPQKTMPRGILGSLSVAIV
                     LFIAVALVLVGMFHYSQYANNAEPVGWALRQSGHGVVAAIVQAISVIGMFTALIGMML
                     AGSRLLYSFGRDGLLPSWLSHLNDKHLPNRALVILTIIGVLIGSMFPFAFLAQLISAG
                     TLVAFMFVSLAMYRLRKREGKDLPIPAFKLPLYPVLPAITFVLVLLVFWGLGFEAKLY
                     TLIWFIVGIILYLSYGLRHSKKNDVAEYHPPK"
BASE COUNT         2449 a         1170 c         1231 g         2168 t
ORIGIN      
        1 tgaataatta taatttacat ttaccactgc ttgctttaac atatacatgt aagcactaac
       61 tatagtattt tgttacagcg tatcaattaa aacaggaagt tatctatggc gttgatattt
      121 taaagaaata tgtcagtttt atgtaacttt catattgatg atacacattt acaatttttg
      181 gagaggtgaa accaatggct aagtctaata gtaaagacat cgttttaatt ggagccggtg
      241 tacttagcac aacatttggt tcaatgttaa aagaaattga gccagactgg aatatccacg
      301 tttacgaacg cttggatcgt cctgcaatcg aaagttcaaa cgaaagaaat aatgctggta
      361 cgggtcatgc agcattatgt gagttgaact acacagtttt acaacctgat ggttctatcg
      421 acatcgaaaa agcgaaagtg attaacgaag agtttgagat ttcaaaacaa ttctggggtc
      481 acttagtgaa aagcggtagc atcgagaacc caagagaatt tatcaatcca ttaccacaca
      541 tcagttatgt tagaggtaaa aacaatgtta aattcttaaa agatcgttac gaagcgatga
      601 aagctttccc tatgttcgat aatatcgaat atactgaaga catcgaagta atgaaaaaat
      661 ggattccatt gatgatgaaa ggccgtgaag ataaccctgg tatcatggcg gcaagtaaaa
      721 ttgacgaagg tacagatgta aacttcggtg aattaacacg taaaatggct aaaagcattg
      781 aagcacatcc aaatgctaca gtgcaattta accatgaagt tgttgatttt gaacaattat
      841 caaatggtca atgggaagtt actgttaaaa atcgcctaac tggtgagaaa ttcaaacaag
      901 taactgacta tgtattcatc ggtgctggcg gtggagcgat tccattatta caaaaaacag
      961 gtatccctga aagtaaacat ttgggtggat tccctatcag tggtcaattc ttagcttgta
     1021 caaacccaca agttattgaa caacacgatg ccaaagttta tggtaaagag ccacctggta
     1081 caccaccaat gactgtacct catttagata cgcgttacat tgatggtcaa agaacattat
     1141 tatttggacc atttgctaat gttggaccta aattcttgaa aaatggttct aacttagatt
     1201 tattcaagtc tgttaaaaca tacaacatta caactttatt agcagcagca gttaaaaact
     1261 tacctttaat taaatactca tttgatcaag tgattatgac aaaagaaggt tgtatgaacc
     1321 acttacgtac tttctatcca gaagcacgta atgaagattg gcaattatac actgctggta
     1381 aacgtgtaca agttatcaaa gatacacctg aacacggtaa aggattcatc caattcggta
     1441 cagaagtggt taactcacaa gaccacactg taattgcatt attaggtgaa tcaccagggg
     1501 cttcaacttc agtttcagtt gcgttagaag tattagaacg taacttccct gaatacaaaa
     1561 ctgaatgggc acctaaaatc aagaaaatga ttccatcata cggtgaatca ttaattgaag
     1621 acgaaaaatt aatgagaaaa atccgtaaac aaacttcaaa agacttagaa ttaggttact
     1681 acgaaaacta aattctaata gctatattta aaatcggaca actgagaaat cttaaagatt
     1741 tttcagctgt ccgatttttt tacgtaatct aatacatacg gcaaattcaa ctttaatcaa
     1801 aaaagactca tacacaatgc ccttaaagca catgtatgag tcctttttag tagtttatat
     1861 caaaaaatac gtttaatgta taaattagtt tttgtttaca gatgcgtcgt agattgattc
     1921 tacagcatca cctaaagctt tatcgaattc ttctttagat tgatcagctc ttaaatcact
     1981 agctaatgca cgtgagaaac ttgcgataag ttcatcgtta tctttaagta attcatttgc
     2041 tttttctctg ctgtaaccac ctgataatac aacgacacga acaacattag gatgttcagc
     2101 taactctttg tataagtttg gttcagtagg aattgttaat ttcaacatta ctaattgatc
     2161 agcatttaag ctatctaaac cttttttaag ttcagctttt aatacttttt caatttcagc
     2221 tttgtctttt gcattaatat taacttctgg ttcgataatt ggcactaaac ctttagcaat
     2281 aatttgttta gcaacttcaa attgttgttc aacaacgtct ttgatacctt gctcatttaa
     2341 ttctaaaatg ttagaacgca tttttgtacc aaaaatgtga cgttcgtttg cacggtctaa
     2401 taaactgtct aagttgtcga ttggtttcat taattgaaca ccattttgct cttcagcaag
     2461 acctttgtct acttttaaga acggaacaac acctttatca gctaagtaat ctgcagtgta
     2521 tttgccttct acttcgcgat ccattgtttg ttcgaaaaga atagcaccca aaattttatc
     2581 tggtgagaat gaaggtgaag ttaccacacg tgtacgcata tcgtgaacaa gttggaacat
     2641 ttcgtcttca ttgctatatt gatcttcgtt tacaccatat tctttaagtg cttttggtgt
     2701 actaccacca ctttggtcta atgcggcaat aaagcctttt ccatttttca ttttttctaa
     2761 ttgctcttta ttcatacttt ccactcctta acttttcaat acaccttcag tatgataaaa
     2821 atgagaacat ttctcaagtc ataaaccttg aaaagtgtat aaaatgtgaa aaataattgt
     2881 cagtttaatt aaaaatatta ttttattcta ggtatgacta acgccattaa tgacataaag
     2941 aaaatatgtg taataatcca accgattaat tctgtcacac taaattgaaa aattggacgt
     3001 tgcgcaataa atactaaaaa gggatacaat gctataaata agaaaaataa agggatataa
     3061 catagatagt aaagcctttt agaagtatga aacagcaaca caaatacaaa ataaatgacg
     3121 ctaccaatta gtaagtgaca tattatttct agaatatatg gtgtattgtc agaaggaatt
     3181 agaaaatcta aattcaataa taaagctttt aaatgtgatt gattgcttat taagtcaaac
     3241 aacatggtca tccatagcaa catgaatgat atgaaaatag cagcaatgaa atggaacaat
     3301 acttttaaac gcattcgtat cacctcagag tcaaatggaa tatagtaaac aattccctaa
     3361 tcgaacaaaa ttatgcgcat aaacaaagta gattgatata aaattcttaa ttatcagaat
     3421 atatttacaa atctgaattt tattagtata ttggatagta ttcatagagg catgacggta
     3481 tttgagcagg attttaaatc ggattttata atcgatttaa gagaggccac tttgcttgca
     3541 cattaatact gtcaatggga ggggaatgta tatgagtaaa gcacatcaat taattcaaga
     3601 ggatgaacat tattttgcga aatcaggacg tattaaatat tatccgttag tgattgatca
     3661 tggatatgga gcaacattgg ttgatattga ggggaagaca tatatcgatt tgttatcgag
     3721 tgcaagttct caaaacgtag gtcatgcacc tagagaagta acagaagcga taaaggcaca
     3781 agttgataaa tttattcatt atacaccagc atatatgtat catgaaccgt tggtacgttt
     3841 agctaagaag ctttgtgagc ttgcacctgg agattttgaa aaaagagtga ccttcggatt
     3901 aaccggatca gacgcaaatg atggcatcat taaatttgcc agagcatata cagggcgtcc
     3961 ttatatcatt agtttcacta atgcatatca tggttcaact tttggctcat tgtctatgtc
     4021 agctattagt ttaaatatgc gcaaacatta tggtccgtta ttgaatggtt tttatcatat
     4081 tccgtttcca gataaatatc gtggtatgta cgagcagcca caagctaatt cagtagaaga
     4141 atatttagca cccttaaaag aaatgtttgc gaaatatgta cctgctgacg aagtagcatg
     4201 tattgttatt gaaacgatac aaggcgatgg tggactttta gaaccagttc cagggtattt
     4261 tgaagcgtta gaaaagattt gtcgtgaaca tggtatttta atcgctgtcg atgatattca
     4321 acaaggtttt gggcgaacag gtacatggag ttcagtctcg cattttaatt ttacgcctga
     4381 tttaatcact ttcggaaaat ccttagcagg tggtatgccc atgtcagcaa ttgttggacg
     4441 caaagagatt atgaattgtt tagaagcacc agcacattta tttacaacag gtgctaatcc
     4501 agttagttgt gaagctgcat tagccacaat tcaaatgatt gaagatcagt cgcttcttca
     4561 ggctagtgcg gaaaaagggg aatatgttag gaaacgaatg gatcaatggg tatctaaata
     4621 caatagtgta ggcgatgtta gaggtaaagg tctgagcatt ggtattgata ttgtttccga
     4681 caaaaaactc aaaacacgtg atgccagtgc ggcacttaaa atttgtaatt actgctttga
     4741 gcatggcgta gttattatag ctgtagcagg aaatgtgttg cgattccaac cgccattggt
     4801 gataacatat gagcaattag acacggcgtt aaacactata gaagatgcac tgactgcttt
     4861 ggaagcaggt aacttagatc aatatgacat atctggacaa ggttggtaat agcgattatc
     4921 ttaatataaa ataaaaaatc atttccacat ctggatgtta atcagatggg aaatgatttt
     4981 tttgattttt tattttggtg ggtggtattc agctacatca tttttcttag aatgtctaag
     5041 tccataactt aaatatagaa tgataccaac aataaaccaa attaaagtgt ataatttcgc
     5101 ttcgaatcct aatccccaga atactagcaa tactaaaaca aatgtaattg ctggtaacac
     5161 aggatataaa ggtaatttaa atgcaggaat tggtagatct ttaccttcac gctttctcaa
     5221 acgatacatt gctaatgaaa cgaacataaa tgcaacaagt gtacctgctg aaattaattg
     5281 tgctaaaaat gcgaatggga acatagaacc aattaaaaca ccaataatag taagtataac
     5341 tagtgcgcga ttaggtaaat gtttgtcgtt taaatggctt aaccatgaag gtaataagcc
     5401 gtcacgtcca aatgaataaa gtaaacgtga gcctgctaac atcataccaa ttaatgctgt
     5461 aaacataccg ataacagaga tagcttgaac aatagctgct acaacaccat gaccactttg
     5521 acgtaaagcc caaccaacag gttcagcatt gtttgcgtat tgtgagtaat ggaacatacc
     5581 aactaacaca agtgctacag caataaataa tacgatagca acacttaaag aaccaagaat
     5641 acctctaggc attgtctttt gagggtcaag tgcttctgct gagtttgctg cgatagaatc
     5701 gaaaccgata tacgctaaga aaatcattga aacaccagca tatatgcctt gccatccacc
     5761 aaagtcacct gtagcagtta ctttgtgttc tggaataaat ggcacatagt tactaacatt
     5821 tattgctgtt aaacctacga tgacaaataa aataatagct aatactttta aaataactaa
     5881 aatgttttcc atacgtgctg cttctgacat accacgtgat agtaataatg cagttaataa
     5941 aataacgata gcagcaataa tatcgataaa accgccattc gtaccaaatg gatttgataa
     6001 tgctgcaggt aattcgatgc caattggttt cacaagtccg cgtaaattcg ctgagaatcc
     6061 tgatgcaaca aaggctacgg cgataaaata ttcagctaat agagcccaac cggcaaccca
     6121 tccaaaaaat tcaccaaata atacattgac ccaagaatag gctgaacctg caaatggcat
     6181 agcggcagcc atttctgcat aagtaaatgc aactaaacca gcaacaatag cagcgagtaa
     6241 gaatgataac gcaacggccg gtcctgcatg ttctgcagca acaatgccag gtagcgtaaa
     6301 gatagatgtc gatacaattg ttcctacacc taaagctaag aaatcacgca cccgaagtgt
     6361 acgctttaaa tgaccatctt tattttgata gatagccgga tcctcttttc gtgctatttt
     6421 attgaaaaaa cttcccataa actttcctcc caaacattca taaacaattc tatacggtgt
     6481 tttttaatat gttatatcat agcacaaata atcaatattt tgtctaaaaa ttctgaaaaa
     6541 tcacaacttt atgttacgta ttaatgactt gtcttgataa catccataga ttttttaaat
     6601 gataaaactg attataacag atattaaatg aataagtact atttttgcta attttctaac
     6661 aattttgcac attatatttt taaaatcaat ttcatgttta tgtctgattg gctagtttgt
     6721 atgaaatgta attctttgac tataaaatga tgcatcaaat atgatgaggg taaggcggaa
     6781 aataggaggt tatatagtaa gtcatttaag tcgaatagcc atccttttta aaatgaaaaa
     6841 aatagaaagc tcaatagttt gttaaaggct ttaaatagcg tcattacgca attttagaat
     6901 gctaaaaatt gtcacaaatt tcaaattacg tgctaaagct tgagatatca atatttattg
     6961 gcgatagagt gtaatttgac tccgcttaca tctcagtttt atgtttgtaa tttggtag
//
LOCUS       DANBWH010000143         6963 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.147, whole genome shotgun sequence.
ACCESSION   DANBWH010000143 DANBWH010000000
VERSION     DANBWH010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6963
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.147"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            101..760
                     /gene="arlR"
                     /locus_tag="PPY92_002005"
     CDS             101..760
                     /gene="arlR"
                     /locus_tag="PPY92_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor ArlR"
                     /protein_id="HDJ2625992.1"
                     /translation="MTQILIVEDEQNLARFLELELTHENYNVDTEYDGQDGLDKALSH
                     YYDLIILDLMLPSINGLEICRKIRQQQSTPIIIITAKSDTYDKVAGLDYGADDYIVKP
                     FDIEELLARIRAILRRQPQKDIIDVNGITIDKNAFKVTVNGAEIELTKTEYDLLYLLA
                     ENKNHVMQREQILNHVWGYNSEVETNVVDVYIRYLRNKLKPYDRDKMIETVRGVGYVI
                     R"
     gene            757..2112
                     /gene="arlS"
                     /locus_tag="PPY92_002006"
     CDS             757..2112
                     /gene="arlS"
                     /locus_tag="PPY92_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase ArlS"
                     /protein_id="HDJ2625993.1"
                     /translation="MTKRKLRNNWIIVTTMITFVTIFLFCLIIIFFLKDTLHNSELDD
                     AERSSSDINNLFHSKPVKDISALDLNASLGNFQEIIIYDEHNNKLFETSNDNTVRVEP
                     GYEHRYFDRVIKKRYKGIEYLIIKEPITTQDFKGYSLLIHSLENYDNIVKSLYIIALA
                     FGVIATIITATISYVFSTQITKPLVSLSNKMIEIRRDGFQNKLQLNTNYEEIDNLANT
                     FNEMMSQIEESFNQQRQFVEDASHELRTPLQIIQGHLNLIQRWGKKDPAVLEESLNIS
                     IEEMNRIIKLVEELLELTKGDVNDISSEAQTVHINDEIRSRIHSLKQLHPDYQFDTDL
                     TSKNLEIKMKPHQFEQLFLIFIDNAIKYDVKNKKIKVKTRLKNKQKIIEITDHGIGIP
                     EEDQDFIFDRFYRVDKSRSRSQGGNGLGLSIAQKIIQLNGGSIKIKSEINKGTTFKII
                     F"
     gene            2396..5194
                     /locus_tag="PPY92_002007"
     CDS             2396..5194
                     /locus_tag="PPY92_002007"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDJ2625994.1"
                     /translation="MTNERKEVSEAPVNFGANLGLMLDLYDDFLQDPSSVPEDLQVLF
                     STIKNDDSIVPALKSTSSQNSDGTIKRVMRLIDNIRQYGHLKADIYPVNPPKRKHVPK
                     LEIEDFDLDQQTLEGISAGIVSDHFADIYDNAYEAILRMEKRYKGPIAFEYTHINNNT
                     ERGWLKRRIETPYKVTLNNNEKRALFKQLAYVEGFEKYLHKNFVGAKRFSIEGVDALV
                     PMLQRTITIAAKEGIKNIQIGMAHRGRLNVLTHVLEKPYEMMISEFMHTDPMKFLPED
                     GSLQLTAGWTGDVKYHLGGIKTTDSYGTMQRIALANNPSHLEIVAPVVEGRTRAAQDD
                     TQRAGAPTTDHHKAMPIIIHGDAAYPGQGINFETMNLGNLKGYSTGGSLHIITNNRIG
                     FTTEPIDARSTTYSTDVAKGYDVPIFHVNADDVEATIEAIDIAMEFRKEFHKDVVIDL
                     VGYRRFGHNEMDEPSITNPVPYQNIRKHDSVEYVFGKKLVNEGVISEDEMHSFIEQVQ
                     KELRQAHDKINKADKMDNPDMEKPAELALPLQADEQSFTFDHLKEINDALLTYPDGFN
                     ILKKLNKVLEKRHEPFNKEDGLVDWAQAEQLAFATILQDGTPIRLTGQDSERGTFSHR
                     HAVLHDEQTGETYTPLHHVPDQKATFDIHNSPLSEAAVVGFEYGYNVENKKSFNIWEA
                     QYGDFANMSQMIFDNFLFSSRSKWGERSGLTLFLPHAYEGQGPEHSSARLERFLQLAA
                     ENNCTVVNLSSSSNYFHLLRAQAASLDSEQMRPLVVMSPKSLLRNKTVAKPIDEFTSG
                     GFEPILTESYQADKVTKVILATGKMFIDLKEALAKNPDESVLLVAIERLYPFPEEEIE
                     ALLAQLPNLEEVSWVQEEPKNQGAWLYVYPYVKVLVADKYDLSYHGRIQRAAPAEGDG
                     EIHKLVQNKIIENALKNN"
     gene            5208..6476
                     /gene="sucB"
                     /locus_tag="PPY92_002008"
     CDS             5208..6476
                     /gene="sucB"
                     /locus_tag="PPY92_002008"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDJ2625995.1"
                     /translation="MPEVKVPELAESITEGTIAEWLKNVGDSVEKGEAILELETDKVN
                     VEVVSEEAGVLSEQLASEGDTVEVGQAIAIIGEGSGNASKENSNDNTPQQNEETNNKK
                     EETTNNSVDKAEVNQANDDNQQRINATPSARRYARENGVNLAEVSPKTNDVVRKEDID
                     KKQQAPASTQTTQQAPAKEEKKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAMLT
                     TFNEVDMTNVMELRKRKKEQFMKDHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGDD
                     MITKQYYDIGVAVSTDDGLLVPFVRDCDKKNFAEIEAEIANLAVKAREKKLGLDDMVN
                     GSFTITNGGIFGSMMSTPIINGNQAAILGMHSIITRPIAIDQDTIENRPMMYIALSYD
                     HRIIDGKEAVGFLKTIKELIENPEDLLLES"
BASE COUNT         2629 a         1024 c         1257 g         2053 t
ORIGIN      
        1 aacgtaaaca aactaattta aaaagcaact attgtataga aaaatacaaa atttaaaata
       61 tattacctta ttagaaaaag tcgtaatatg aggtgtacaa atgacgcaaa ttttaatagt
      121 agaagatgaa caaaacttag caagatttct tgaattggaa ctcacacatg aaaattacaa
      181 tgtggacaca gagtatgatg gacaagacgg tttagataaa gcgcttagcc attactatga
      241 tttaatcata ttagatttaa tgttgccgtc aattaatggc ttagaaattt gtcgcaaaat
      301 tagacaacaa caatctacac ctatcattat aattacagcg aaaagtgata cgtatgacaa
      361 agttgctggg cttgattacg gtgcagacga ttatatagtt aagccgtttg atattgaaga
      421 acttttagca agaattcgtg caattttacg tcgtcagcca caaaaggata ttatcgatgt
      481 caacggtatt acaattgata agaacgcttt taaagtgacg gtaaatggcg cagaaattga
      541 attaacaaaa acagagtatg atttactata tcttctagct gaaaataaaa accatgttat
      601 gcaacgggaa caaattttaa atcatgtatg gggttataat agtgaagtag aaacaaatgt
      661 cgtagatgtt tatataagat atttacgaaa caagttaaaa ccatacgatc gtgacaaaat
      721 gattgaaaca gttcgtggcg ttgggtatgt gatacgatga caaaacgtaa attgcgcaat
      781 aactggatta ttgttaccac gatgattacg tttgtcacga tatttttgtt ttgtttaatt
      841 attatttttt tcttgaaaga tacactgcat aatagtgagc ttgatgatgc agaacgaagc
      901 tcaagcgata ttaataattt atttcattct aagcctgtta aagatatatc tgcattagac
      961 ttgaatgcat ctttaggtaa ttttcaagag ataattattt atgatgagca taataataaa
     1021 ttatttgaga catcgaatga taacacagtg agagttgaac caggttatga acaccgttat
     1081 tttgaccgcg taataaaaaa acgctataaa ggcattgaat atttaattat taaagaacca
     1141 attacaacgc aagatttcaa agggtatagc ttgttaattc attcactaga aaattatgat
     1201 aacatcgtaa aatcattgta tatcattgcg ctggcatttg gagtgattgc aacaattata
     1261 actgccacaa tcagttatgt attttcaaca caaattacta aaccgcttgt cagtttatca
     1321 aataaaatga ttgagattcg acgagatggt tttcaaaata aattgcaatt aaatacaaat
     1381 tatgaagaaa tagataattt agcaaatacg tttaatgaga tgatgagcca aattgaagaa
     1441 tcatttaatc aacaaagaca atttgttgaa gatgcgtcac atgaattacg aacaccatta
     1501 caaattattc aaggccattt aaatttgatt cagcgatggg gaaaaaaaga cccagcagta
     1561 ttagaagaat cgttaaatat ttctattgaa gaaatgaatc gtatcataaa attagtcgaa
     1621 gaattacttg aattgactaa aggagatgta aatgacattt cttctgaagc gcagaccgtg
     1681 catattaatg atgaaattcg ctcgcgaata cactcattaa aacaattgca tcctgattat
     1741 caatttgata cggatctgac atctaaaaat ctagaaatta aaatgaaacc tcatcaattc
     1801 gaacaattat ttttaatctt tattgataat gcaatcaaat atgatgtgaa gaataagaaa
     1861 attaaagtta agacaaggtt aaaaaataag caaaaaataa ttgaaattac agatcatgga
     1921 attggtattc cagaggaaga tcaagatttc atttttgatc gcttttatcg agtggataaa
     1981 tctcgttcaa gaagtcaagg cggtaatgga ctcggattat ctattgctca aaaaatcatt
     2041 caattaaacg gaggatcgat taaaattaaa agtgaaatta ataaaggaac aacgtttaaa
     2101 atcatatttt aatcatgtct gagacgtcaa tcaaagtcat aggatcaatt ttttaagtac
     2161 acattagctg tgactaatgt ataagaacaa ctataaaaca aataaacagt ggttctttat
     2221 catttctgtt gtactcccaa aatttacaat aaaatacatc tataaaccta gaagaatcaa
     2281 cgcttttgtt gattcttctt tttagcagat aaataggtaa atctacttta acaaataact
     2341 aaatagtgat attattacat tgtaagcgtt tcaacatttt tgtggagggt gtaaaatgac
     2401 taacgaaaga aaagaagttt cagaggctcc tgtaaacttc ggtgcgaatt taggtctaat
     2461 gttagatcta tatgatgact ttttacaaga tccatcatct gtaccagaag atttacaagt
     2521 cttattcagc acaattaaga atgatgactc aattgtacca gctttaaaaa gtacaagtag
     2581 tcaaaatagc gacggcacaa ttaagcgtgt catgcgtcta attgataata ttcgccaata
     2641 cgggcatctt aaagccgata tttatcctgt aaatcctcca aaaaggaaac atgtacctaa
     2701 attagagatt gaagactttg atttagatca acagactttg gaaggtatat cagcaggaat
     2761 tgtttcagat cactttgccg acatttatga taatgcttat gaagcaattt taagaatgga
     2821 aaaacgttac aaaggaccaa ttgcatttga gtatacacat attaataaca ataccgaacg
     2881 tggttggtta aaaagaagaa ttgaaacgcc atataaagta acgttaaata ataacgaaaa
     2941 aagggcacta ttcaaacaat tagcgtatgt tgaagggttt gaaaaatatc ttcataaaaa
     3001 cttcgttggt gcaaagcgtt tttcaattga aggggtagac gcacttgtac cgatgttaca
     3061 acgtactatt acgattgctg cgaaagaagg tattaaaaat atacaaatag gcatggctca
     3121 ccgtggacgt ttaaacgttt taacgcatgt cttagaaaaa ccgtacgaaa tgatgatttc
     3181 agaatttatg catacagatc caatgaaatt cttacctgaa gatggtagct tgcagttaac
     3241 tgctggatgg actggtgatg tgaaatatca ccttggtggc attaaaacta ctgattcata
     3301 cggtacaatg cagcgtattg cactggctaa caatccaagt cacttggaaa ttgttgcacc
     3361 tgttgttgag gggcgtacga gagcagcaca agatgataca caacgagctg gggctccgac
     3421 gactgatcat cataaagcaa tgccaattat tatacatggc gatgctgctt atcctggtca
     3481 aggaattaac ttcgaaacaa tgaacttagg aaacttgaaa ggctattcta cgggtggttc
     3541 attgcatatt attactaaca atagaattgg atttactaca gaaccaattg atgcacgttc
     3601 aacaacttat tctacagatg tggccaaagg ttatgatgtg ccaatattcc atgtcaatgc
     3661 tgatgacgtt gaagctacta ttgaagcaat tgatattgca atggaattta gaaaagagtt
     3721 tcataaagac gtcgttattg atttagtagg ttatcgtcgt ttcggacata acgaaatgga
     3781 tgaaccatca attactaatc cagttcctta tcagaatatt cgcaaacatg actctgttga
     3841 atatgtgttt ggtaaaaagc ttgttaatga aggtgtcatt tcagaagatg aaatgcattc
     3901 atttatagaa caagtccaaa aggaactaag acaagctcat gataaaatta ataaagctga
     3961 taaaatggat aatccagata tggaaaaacc tgcagaactt gcattaccgt tacaagcaga
     4021 cgaacaatca tttacgtttg atcatttgaa agaaataaat gatgcattgt taacatatcc
     4081 ggatggcttt aacattttga aaaagttaaa caaagttctt gagaagcgtc atgagccgtt
     4141 taataaagaa gatggcttag ttgattgggc acaagcagaa caacttgcat ttgcgacaat
     4201 tttacaagat ggtacaccga ttcgcttaac tggtcaagat agtgaacgtg gtacattcag
     4261 tcataggcat gccgtgttac atgatgagca aacaggtgaa acatatacac ctttacatca
     4321 tgttcctgat caaaaagcga catttgatat acacaattct ccgctttcag aagcagcagt
     4381 agttggtttt gaatacggct ataatgtgga aaacaaaaaa agcttcaata tttgggaagc
     4441 gcaatatggt gattttgcaa atatgtcaca aatgattttt gacaacttct tattcagttc
     4501 tcgctcaaaa tggggagaac gttcaggatt aacattattc ttacctcatg catatgaggg
     4561 tcaaggacct gaacattcat cagcaagatt agagcgattt ttacaattag ctgctgaaaa
     4621 taattgcaca gttgtcaact tatctagttc aagtaattat ttccacttat tgcgtgcaca
     4681 agcagctagt ttagattctg aacaaatgcg accattggtt gttatgtcac caaaaagctt
     4741 attgagaaat aaaacagttg ccaaaccaat tgatgagttt acttctggtg gatttgagcc
     4801 aattttgaca gaatcatatc aagcggataa ggttacaaaa gttattttag caactggtaa
     4861 aatgttcatt gatttaaaag aagcattagc taaaaatcca gacgaatccg tgttactcgt
     4921 tgcgattgaa agattgtatc cattcccaga ggaagagatt gaagcattac tagcacaatt
     4981 gccaaacctt gaagaagtgt catgggtaca agaagaacct aaaaatcaag gtgcatggtt
     5041 atatgtctat ccatatgtta aagtgctagt tgcagataaa tatgatttaa gttatcatgg
     5101 cagaattcaa agggctgctc cagctgaagg cgatggagaa attcataaac ttgttcaaaa
     5161 taaaattata gaaaatgcat taaaaaataa ctagggggaa ataagtcatg ccagaggtta
     5221 aagttccaga attagcagaa tctattacag aaggtaccat tgcagaatgg ttgaaaaacg
     5281 taggggatag cgtagaaaaa ggtgaagcta ttcttgaatt agaaactgat aaagttaatg
     5341 tcgaagttgt atctgaagaa gcaggtgtat tatctgaaca acttgcaagt gaaggcgaca
     5401 ctgtagaagt tggacaagca attgctatca tcggcgaagg tagtggcaat gcttctaaag
     5461 aaaatagtaa cgacaatact ccacaacaaa atgaagaaac aaataataaa aaagaagaaa
     5521 caacaaataa ttcggtagat aaagctgaag tcaatcaagc aaatgatgac aatcagcaac
     5581 gtattaatgc tacgccttct gcgcgtcgat atgctcgtga aaatggtgtg aatcttgctg
     5641 aagtaagtcc gaaaacaaat gatgtggttc gtaaagaaga tattgataag aaacaacagg
     5701 caccggcatc aacacaaaca acacaacaag cacctgcaaa agaagagaaa aaatacaatc
     5761 aatatcctac aaaaccagtg attcgtgaaa aaatgtcacg cagaaagaaa acagctgcca
     5821 aaaaattatt agaggtatct aataatacag ctatgttaac aacatttaac gaagttgaca
     5881 tgacaaatgt tatggaattg cgtaaacgta agaaagaaca atttatgaaa gatcatgatg
     5941 gtactaaatt aggatttatg tcattcttta ctaaagcttc tgtagcagct ttgaaaaagt
     6001 atccagaagt taatgcagaa atcgatggcg acgacatgat tacgaaacaa tattatgata
     6061 ttggtgtagc tgtttctaca gatgatggat tattagtacc atttgtaaga gattgtgata
     6121 aaaagaattt tgcagaaatc gaagcagaaa ttgctaattt agcagttaaa gcacgagaga
     6181 aaaaacttgg cttagatgat atggttaatg gttcatttac gattacaaat ggcggtattt
     6241 ttggatcaat gatgagtacg ccaattatca atggtaatca agctgcaatc ttaggcatgc
     6301 attcaattat tacaagacca attgcgattg atcaagatac aatcgaaaat cgtccaatga
     6361 tgtatattgc attaagctat gatcatagaa ttattgacgg taaagaagca gttggattct
     6421 taaaaacaat taaagaatta attgaaaacc cagaagactt attattagaa tcttaatcca
     6481 caacacaaaa tagtttacta ttcctaaaaa cgtaatcaaa tcaataacaa acagcagtaa
     6541 gattattttc tagtcgaaat atcttactgc tgtactttat ttttataatg cttcgtatta
     6601 aaatgtgagt ttaattttga caacttaaaa aatatatcat ttttattgaa atagactcac
     6661 aatagaaata taggtggaat ggcgtgtttc aatatgctaa tgaaatcatt ttagataata
     6721 acaagcgatg aaatttatga attctataag cgcctttagt taatttaaaa tcatctgaac
     6781 aagatgtaaa ttcactttat ggatacgaca tgtatatgtt ttttatgtac tacataaaac
     6841 gaaatattca attgctatat acagatactc attaatgaca tactcaatag caaccataac
     6901 aatattatgt tacctattta aatgaaatag ttcttttaaa gaaatacatt tttcacatat
     6961 taa
//
LOCUS       DANBWH010000144         6945 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.148, whole genome shotgun sequence.
ACCESSION   DANBWH010000144 DANBWH010000000
VERSION     DANBWH010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6945
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.148"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..418
                     /locus_tag="PPY92_002009"
     CDS             <1..418
                     /locus_tag="PPY92_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HDJ2625996.1"
                     /translation="ECLIYIILGITIISIIYSDFSHYLLLIILFSLVILQYILVVGTI
                     SMVSPNILISLGISIVYWIGSVILVAINKNIFGIVAPFEASNTMYRAVEKILNNESTF
                     ICPTEIINTVSFFVLLFIVNTIVLLLSRKRWLKIGM"
     gene            complement(580..1200)
                     /gene="srtA"
                     /locus_tag="PPY92_002010"
     CDS             complement(580..1200)
                     /gene="srtA"
                     /locus_tag="PPY92_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase SrtA"
                     /protein_id="HDJ2625997.1"
                     /translation="MKKWTNRLMTIAGVVLILVAAYLFAKPHIDNYLHDKDKDEKIEQ
                     YDKNVKEQASKDNKQQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATPEQLNRGVS
                     FAEENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRD
                     VKPTDVEVLDEQKGKDKQLTLITCDDYNEKTGVWEKRKIFVATEVK"
     gene            1394..1885
                     /locus_tag="PPY92_002011"
     CDS             1394..1885
                     /locus_tag="PPY92_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDJ2625998.1"
                     /translation="MIRCAKKEDLNAILAIYNDAIINTTAVYTYKPQTIDERIAWFET
                     KQRNHEPIFVFEENGSVLGFATFGSFRPWPAYQYTIEHSIYVDASARGKGIASQLLQR
                     LIVEAKAKGYRTLVAGIDASNEASIKLHQKFNFKHAGTLTNVGYKFDYWLDLAFYELD
                     LKD"
     gene            complement(2029..2199)
                     /locus_tag="PPY92_002012"
     CDS             complement(2029..2199)
                     /locus_tag="PPY92_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2625999.1"
                     /translation="MLRLSIIFIAFIINTTITYGYTTEGTWVNLLFKSLSLSMIIVFM
                     FYYIRFVIEKKR"
     gene            complement(2261..2359)
                     /locus_tag="PPY92_002013"
     CDS             complement(2261..2359)
                     /locus_tag="PPY92_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626000.1"
                     /translation="MIKKKHLSGSLVPDRCFLIIFHKVEKFMGNTC"
     gene            complement(2378..3277)
                     /gene="sdaAA"
                     /locus_tag="PPY92_002014"
     CDS             complement(2378..3277)
                     /gene="sdaAA"
                     /locus_tag="PPY92_002014"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HDJ2626001.1"
                     /translation="MFDSIRETIDYAVENNMSFADIMVKEEMELSGKSRDEVRAQMKQ
                     NLDVMRDAVIKGTTGDGVESVTGYTGHDAAKLRDYNETHHALSGYEMIDAVKGAIATN
                     EVNAAMGIICATPTAGSSGTIPGALFKLEKTHDLTEEQMIDFLFTSALFGRVVANNAS
                     VAGATGGCQAEVGSASAMAAAAAVAIFGGSPEASGHAMALAISNLLGLVCDPVAGLVE
                     IPCVMRNAIGSGNALISADLALAGIESRIPVDEVIEAMDKVGRNLPASLRETGLGGLA
                     GTPTGEAIKRKIFGTAEDMVKNN"
     gene            complement(3291..3971)
                     /gene="sdaAB"
                     /locus_tag="PPY92_002015"
     CDS             complement(3291..3971)
                     /gene="sdaAB"
                     /locus_tag="PPY92_002015"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HDJ2626002.1"
                     /translation="MAKSYDYQSAFDIIGPVMMGPSSSHTAGAVKIGNSARAILGDIP
                     KNIEIHYYESFAQTHQGHGTDVAIVGGAMGYSTFDNRIKSSLEIAADDGIKIDIIEEE
                     GDSIGQHPNCAYIKSSRNDGRYIEIIGISIGGGTIKIKGIHINGLEVDLNHGLPILVV
                     DGNMTKAQVNHFINDINDMKLDCKDEFIKIDEDKCLVVIPLNKAISESTLNTIKEKYS
                     DVNVSYIN"
     gene            complement(3974..5017)
                     /locus_tag="PPY92_002016"
     CDS             complement(3974..5017)
                     /locus_tag="PPY92_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDJ2626003.1"
                     /translation="MDILLGVGTLVLVLVIMTLFLKYAPYGKQGLQALSGAACATFLP
                     QAFLSYAIGGVFDIKFLQDIGDLAGSLSGIAVGILTCLNLGVAPVFAVIVGLVLHDFK
                     LLPAFIAAYCVAFLIKWIEKKVPEGLDLIVVILFAPAIAFGLASIITPGVLATLKQIG
                     SAVTAVGDNNPYALAVILGLVIPVTGMTPLSSMVLTSLLGLTGVPMAIGALTCTGSSF
                     VNLILFRKLNIGGPSKAFAVCIEPLTQIDLIAQYPVQLYGTNALIGVVNACIVTFSGL
                     VIGVKGMATPIAGAIVLFGFNNAVTSIVTIATVIIVSIVLAYIIGTLINKFNLMNINF
                     KMPSKKNHIKESV"
     gene            5567..5770
                     /locus_tag="PPY92_002017"
     CDS             5567..5770
                     /locus_tag="PPY92_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626004.1"
                     /translation="MQLSHSVKVAISIYLALIFITFTSYLVIILYTSMTGHDVSHFVL
                     DSQHSHHGSLTQKHLSLPEISFK"
     gene            5953..6888
                     /locus_tag="PPY92_002018"
                     /pseudo
     CDS             5953..6888
                     /locus_tag="PPY92_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501301.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
BASE COUNT         2114 a         1285 c         1049 g         2497 t
ORIGIN      
        1 tgaatgttta atttatatta ttttaggcat cacaattatt tcaataatat attcggattt
       61 ctcacattac ttactattaa taatcttatt ttcactcgtt atcttacaat atatcttagt
      121 tgttggaacg attagtatgg tttcacctaa cattttaatc agcttaggta tcagcattgt
      181 atattggatt ggttcagtca tcttagttgc cattaataaa aatatatttg gtatcgttgc
      241 tccgtttgag gcgagcaaca cgatgtatcg tgccgtagaa aaaatattga ataacgagtc
      301 aacatttata tgtcctactg aaattataaa tacagtatca ttcttcgttc ttctatttat
      361 agttaatact attgtcttat tactaagtag aaagagatgg ttgaaaatcg gtatgtagta
      421 cataaaaaag agcggcatcc attgtcgctc ctttttatta tgtattttta caatctccac
      481 caatgacacg tcgtcattgg tgcctcacac caaacaaaaa aacagtctcg caatcaagac
      541 tgttttccac tcaatatatt catccattag cgtaatggat tatttgactt ctgtagctac
      601 aaagatttta cgtttttccc aaacgcctgt cttttcattg taatcatcac aagtaattaa
      661 tgttaattgt ttatctttac ctttttgttc atctagaact tctacatctg ttggcttaac
      721 atctcttata cttgtcattt tatacttacg tgtttcatta ccaactttaa agtacaccat
      781 actacctttt ttggctgctt taagatttgt aaattgatag ttcggacggt caatgaaagt
      841 gtgtcctgca attgaaatat tttgatcatc tagtgattca ttttcttctg caaagcttac
      901 acctctattt aattgttcag gtgttgctgg tcctggatat actggttctt taatatcagc
      961 atctggaatt tcaatatagc ctgccacttt tgatttatct ttcggaattt gaggtttagc
     1021 ttgctgctta ttgtctttac tcgcctgttc ttttacattt ttatcatatt gttcaatctt
     1081 ttcatcttta tctttatcgt gaagataatt atcgatatgt ggtttagcaa acaaatatgc
     1141 tgccactagg ataagtacta caccagcgat tgtcattaat cgatttgtcc attttttcat
     1201 acgttaaggc tccttttata catttcacaa tgcctattaa catttcatat agtataccat
     1261 taattataaa atgactcata gcaattcatt ttatattata aaatttgcac atatattgtc
     1321 acgttagatt tcattacaca tatttgcatt caaataacga aacgttttta ataattacta
     1381 agggggaatt attatgattc gatgtgctaa aaaagaagat ttaaacgcta tattagcgat
     1441 atacaacgat gccattatta atacaacagc tgtttatact tataaaccgc aaaccataga
     1501 cgaacgtatc gcatggtttg aaacgaaaca acgtaatcat gagcctatct ttgtatttga
     1561 ggaaaatgga agtgtattag ggtttgcgac gttcggttcg tttagacctt ggccagcata
     1621 tcaatatact atcgagcatt ctatttatgt cgatgcttca gctagaggaa aaggtatcgc
     1681 tagtcaatta ttacagcgtt taattgtaga ggcaaaagct aaaggttatc gtacacttgt
     1741 tgctggtatt gatgcttcta atgaagcgag tattaaatta catcaaaaat tcaatttcaa
     1801 gcatgccggc acactgacca atgtaggcta taaatttgat tactggttag atttagcttt
     1861 ttatgaatta gatttaaaag actagtagtg tttaaatcac ataatataaa caagacaacc
     1921 atgttaattc acttaacata acatgctaac atataaaact ataaacctct tagggacaat
     1981 ttacatcgtc ccttttttca tttcaaatac tatccccata gctttgattt aacgcttttt
     2041 ctcaataaca aaacgaatat agtagaacat gaaaacgata atcatgctga gcgataaaga
     2101 tttaaataat agattgaccc acgttccctc agtcgtatat ccatatgtaa tcgttgtgtt
     2161 aatgatgaat gctataaaga tgattgatag tcttagcata tcatcactcc ttttaagtta
     2221 ttttagatat acgggggcgc ttttgcaatc actattttga ttagcatgta tttcccataa
     2281 atttttcaac tttatgaaag ataattaaga agcatctatc tggtactaat gatccagaca
     2341 gatgcttctt ttttatcaat atcttattgt tatctcatta attattttta accatatctt
     2401 cagctgtgcc aaagatttta cgtttaattg cttcgccagt tggtgtgcct gctagtccac
     2461 ccaatccagt ttcacgtaat gatgcaggaa ggttacgacc aaccttatcc attgcttcaa
     2521 taacttcatc aacaggtatt ctactttcaa tacctgctaa tgctaaatct gctgaaatta
     2581 aagcgttacc cgaaccaatt gcatttctca taacacaagg aatttcaaca agtccggcta
     2641 ctgggtcaca aactaaacct aataaattac ttatcgctaa tgccatagcg tgcccggatg
     2701 cttctggtga tcctccgaat atagctactg ctgcagctgc ggccattgca gatgctgaac
     2761 caacttcagc ttggcagcca cctgttgcac cagctacact tgcattgttt gctacgacac
     2821 gcccaaacaa tgctgaagtg aataagaaat caatcatttg ctcttctgtt aaatcatgtg
     2881 ttttttctaa tttaaaaagt gcaccgggaa tggtacccga ggaaccagct gttggcgttg
     2941 cacaaataat acccatcgca gcattgactt catttgttgc aatggcacct ttgactgcat
     3001 caatcatttc atatccagac aaagcatgat gtgtttcatt ataatcacgt agtttagcag
     3061 cgtcatgacc agtgtagccc gttacacttt caacgccatc acctgtcgtc cctttgatta
     3121 ctgcgtctcg catgacatct aaattttgtt tcatttgcgc tcgcacttca tcacgtgatt
     3181 taccgcttaa ttccatttct tctttaacca tgatatccgc aaatgacata ttattttcta
     3241 cggcataatc tatagtctct ctaattgaat caaacatgtt tattccccct ctaatttata
     3301 taggaaacgt ttacgtcact gtatttctct ttaattgtat ttaatgtcga ttctgagatt
     3361 gctttattta atggtattac aaccaagcat ttatcttcat ctatcttaat aaattcatct
     3421 ttacagtcta atttcatatc gttgatatca ttaatgaaat gatttacttg tgctttagtc
     3481 atatttccgt caacaactaa aattggtaat ccatgattta aatctacttc tagtccattt
     3541 atatgaatac ctttaatttt aattgtacca ccaccgattg aaataccgat aatttcaata
     3601 tagcgaccat cattacgaga tgatttaata taagcacagt ttggatgttg accaatacta
     3661 tcgccttctt cttcgatgat atctatttta ataccatcat cagctgcaat ttctaatgaa
     3721 gatttaattc ggttatcaaa tgttgaatat cccattgctc cacccacaat agcgacatct
     3781 gtaccatgtc cttggtgtgt ttgagcaaat gattcataat aatgtatttc aatattttta
     3841 ggaatatctc ccaatattgc gcgtgctgaa ttcccaatct ttactgcacc agccgtatga
     3901 gaacttgaag ggcccatcat aactggtccg ataatatcga aagcactttg ataatcatag
     3961 ctctttgcca taattaaaca ctctccttaa tatgattctt tttgctcggc attttaaagt
     4021 tgatattcat taaattaaac ttattaatta gtgttccaat aatataggct aagacaatgc
     4081 tgacaataat cactgttgcg attgttacaa ttgatgtcac tgcattatta aagccaaaca
     4141 atacgatggc acctgcaatt ggtgttgcca tacctttgac acctattact agtccgctaa
     4201 atgtcacgat acatgcgttg acgaccccaa tcagtgcatt tgtaccatat agttgtactg
     4261 gatattgcgc tattaaatca atttgcgtca atggctcaat acaaactgca aatgcttttg
     4321 acggtccacc aatgtttaat tttcggaata aaataaggtt aacaaatgag ctacctgtac
     4381 atgttagtgc tccaatagcc ataggaacac ctgtcagtcc taataaactt gttaatacca
     4441 ttgaacttag cggtgtcata cctgtaacag gaatcactag tcctaaaatg accgctaatg
     4501 catatggatt gttatcacct accgcagtga cagcactacc tatttgtttt aatgttgcta
     4561 gcacaccagg tgtaatgatt gatgcaagtc cgaaagcaat tgctggtgca aataagatca
     4621 ccacaattaa gtccaagcct tctggaactt tcttttcaat ccatttaatt aaaaaagcta
     4681 cgcaataagc tgcgatgaat gctggtaata atttaaagtc atgtaatact aaaccaacaa
     4741 taaccgcaaa tactggtgca acgcctaagt ttaagcacgt tagaatacct actgcgatac
     4801 cgcttaaact tcctgctaaa tccccaatat cttgtagaaa tttaatatca aatacgccac
     4861 caatagcgta acttaagaat gcttgtggta gaaatgtcgc acaagctgca ccggataatg
     4921 cttgtagtcc ttgtttaccg tacggtgcat actttaaaaa tagcgtcatg atcactaaaa
     4981 ccaagactaa tgtgcctaca cctaacagaa tatccatttc ccaaaaacct ctctctatgt
     5041 ttattttatt ttcagaccat aaacatcgta cacccttaag aaaacgtttt caacttttat
     5101 ctgttatcaa atcaaatatt taagtgaaat atttcatatt tgtgaaagat ttttaaaatg
     5161 gattgtttca aaaactactt atgttgtcat taatatttac taattaactt tttactctat
     5221 atttcaaaca gttgtgtgac agttttttga taactttttt acatctgaaa gtgagtaata
     5281 tttctaaaaa tctttaatat ttatacactt tatctttcga gctatttatt ttttgttttt
     5341 gtgctgtgtt tataaaaccg cacactatat tcaaaaataa cttattacac atatatttgc
     5401 aataagataa ttaaagtagg atattatttt tagttttctg ataggaatga tgatagtcta
     5461 taggttgaat ctttactttt tttaaagcta aatttacatc aacttaacaa tggctggtta
     5521 tactgaagat gaaaatattt agtataactt agtggaggcg ataaaggtgc aattgagtca
     5581 ttccgttaaa gttgcaattt ctatctattt agcacttatc tttataacgt tcacttctta
     5641 tttagtcatt attttatata cgagtatgac tggacatgat gtatcacatt tcgtgttaga
     5701 tagtcagcat tctcatcatg gatctcttac gcaaaaacat ttgagtcttc ctgaaatctc
     5761 atttaaatag tttatcctct gtgtttcaac attcatttcc catatcgatt catttatcta
     5821 tcatctagac cactacatct tagatgattt ttttattttc tcatttcact ctttcttaaa
     5881 agtcgatata atgaattaaa tcattatcat acaccgacat attttatgtt gttggtgtta
     5941 agttttaaag gggtgagata cttggcgaat aatcattcag ctttgcgttt aaaaggaatt
     6001 atacttgcca ttgtcggtgc ttgtttatgg ggattaggtg gtactgtttc tgatttcttg
     6061 ttcaaatata agaatattaa tgtcgattgg tacgtcactg ctcgacttgt agtcagtggt
     6121 gttttcttac ttattatgta caaaatgatg caacccaaac gttcaatatt tagcgtattc
     6181 caagatcgac gtatgttagg caaattactt atcttcagta tactgggcat gttagttgta
     6241 caatatgctt atatggcatc tattaataca ggtaatgctg cgattgcaac attactacaa
     6301 tacattgcgc cagtttatat tattatttgg tttgtcataa gaggcgttgc aaaactaaca
     6361 ttatttgatg tgcttgctat tatcatgaca ctattaggaa catttttatt attaacgaat
     6421 ggttcatttt ctaatttagt cgtcaatcct gcaagtttat tctggggtat tttagctggt
     6481 gtagcactcg ctttttaccc aatttatcct tcagacctac ttaaccgctt cggttagatt
     6541 ctaattgtcg ggtgggcaat gcttatttct ggtgttgcga tgaatttacg ccatccaatt
     6601 tggcacattg atatcactaa atgggacata tcaattatat tatttttaat ctttggtatt
     6661 atcggtggta ccgcactcgc attttatttc tttatcgaca gtttacaata catatcagcg
     6721 aaagaaacaa cattattcgg aactgttgaa cctgtcgtag ccgttatcgc aagcagtcta
     6781 tggttacatg tggcattcaa accatttcaa atcgtaggca tcattcttat tatgatttta
     6841 attttattac tatcacttaa aagacaacct gaagcattag atgaataaga aaactctgat
     6901 aatcacttta gcaagtaact atttttaaca acgtagctac cttgt
//
LOCUS       DANBWH010000145         6927 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.149, whole genome shotgun sequence.
ACCESSION   DANBWH010000145 DANBWH010000000
VERSION     DANBWH010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6927
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.149"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..416
                     /locus_tag="PPY92_002019"
     CDS             <1..416
                     /locus_tag="PPY92_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDJ2626005.1"
                     /translation="KQLEKLITLTNNDLNLVNRRFGQRTDITSEQLELLRILFNYDRL
                     SQYDLTMKISREQSIVSRWIKKLVLKGYITSQQSSEDLRCKELILTDQARTLISQINN
                     ARCELIEARCQCLSEVELDNLNQLLDKLNQRRISL"
     gene            443..553
                     /locus_tag="PPY92_002020"
     CDS             443..553
                     /locus_tag="PPY92_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626006.1"
                     /translation="MSLCTQQKLNHELQAKVAVIVKIDGKQSPLFLLVYV"
     gene            complement(623..4369)
                     /locus_tag="PPY92_002021"
     CDS             complement(623..4369)
                     /locus_tag="PPY92_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDJ2626007.1"
                     /translation="MAKKFNYKLPSMVALTLVGSAVTAHQVQAAETTQDQTTNKNVLD
                     SNKVKATTEQAKAEVKNPTQNISGTQVYQDPAIVQPKTANNKTGNAQVSQKVDTAQVN
                     GDTRANQSATTNNTQPVAKSTSTTAPKTNTNVTNAGYSLVDDEDDNSEHQINPELIKS
                     AAKPAALETQYKAAAPKAKTEATPKVTTFSASAQPRSVAATPKTSLPKYKPQVNSSIN
                     DYIRKNNLKAPKIEEDYTSYFPKYAYRNGVGRPEGIVVHDTANDRSTINGEISYMKNN
                     YQNAFVHAFVDGDRIIETAPTDYLSWGVGAVGNPRFINVEIVHTHDYASFARSMNNYA
                     DYAATQLQYYGLKPDSAEYDGNGTVWTHYAVSKYLGGTDHADPHGYLRSHNYSYDQLY
                     DLINEKYLIKMGKVAPWGTQFTTTPTTPSKPTTPSKPSTGKLTVAANNGVAQIKPTNS
                     GLYTTVYDKTGKATNEVQKTFAVSKTATLGNQKFYLVQDYNSGNKFGWVKEGDVVYNT
                     AKSPVNVNQSYSIKSGTKLYTVPWGTSKQVAGSVSGSGNQTFKASKQQQIDKSIYLYG
                     SVNGKSGWVSKAYLVDTAKPTPTPIPKPSTPTTNNKLTVSSLNGVAQINAKNNGLFTT
                     VYDKTGKPTKEVQKTFAVTKEASLGGNKFYLVKDYNSPTLIGWVKQGDVIYNNAKSPV
                     NVMQTYTVKPGTKLYSVPWGTYKQEAGAVSGTGNQTFKATKQQQIDKSIYLFGTVNGK
                     SGWVSKAYLAVPAAPKKAVAQPKTAVKAYTVTKPQTTQTVSKIAQVKPNNTGIRASVY
                     EKTAKNGAKYADRTFYVTKERAHGNETYVLLNNTSHNIPLGWFNVKDLNVQNLGKEVK
                     TTQKYTVNKSNNGLSMVPWSTKNQVILTGNNIAQGTFNATKQVSVGKDVYLYGTINNR
                     TGWVNAKDLTAPTAVKPTTSAAKDYNYTYVIKNGNGYYYVTPNSDTAKYSLKAFNEQP
                     FAVVKEQVINGQTWYYGKLSNGKLAWIKSTDLAKELIKYNQTGMTLNQVAQIQAGLQY
                     KPQVQRVPGKWTDANFNDVKHAMDTKRLAQDPALKYQFLRLDQPQNISIDKINQFLKG
                     KGVLENQGAAFNKAAQMYGINEVYLISHALLETGNGTSQLAKGADVVNNKVVTNSNTK
                     YHNVFGIAAYDNDPLREGIKYAKQAGWDTVSKAIVGGAKFIGNSYVKAGQNTLYKMRW
                     NPAHPGTHQYATDVDWANINAKIIKGYYDKIGEVGKYFDIPQYK"
     gene            complement(4597..5031)
                     /locus_tag="PPY92_002022"
     CDS             complement(4597..5031)
                     /locus_tag="PPY92_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDJ2626008.1"
                     /translation="MFSKVNNQKMLEDCFYIRKKVFVEEQGVPEESEIDEYESESIHL
                     IGYDNGQPVATARIRPINETTVKIERVAVMKSHRGQGMGRMLMQAVESLAKDEGFYVA
                     TMNAQCHAIPFYESLNFKMRGNIFLEEGIEHIEMTKKLTSLN"
     gene            complement(5186..5656)
                     /locus_tag="PPY92_002023"
     CDS             complement(5186..5656)
                     /locus_tag="PPY92_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499548.1"
                     /note="Essential protein for resistance to osmotic stress
                     from salt; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic stress response protein"
                     /protein_id="HDJ2626009.1"
                     /translation="MSRKTYEKIANINGMFNMLEQQIIHSQDMAHFRSEFFYVNHEHR
                     ENYEALLIYYKNSIDNPIVDGACYILALPEIFNSVDVFESELPFSWVYDENGITETMK
                     SLSIPLQYLVAAALEVTDVNIFKPSGFTMGMNNWNIAQMRIFWQYTAIIRKEAL"
     gene            complement(5704..6921)
                     /locus_tag="PPY92_002024"
     CDS             complement(5704..6921)
                     /locus_tag="PPY92_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase"
                     /protein_id="HDJ2626010.1"
                     /translation="MNKFLKYFLILLALVLIVVPIVFATLLFKTSQDAFESSQDSKNA
                     NRQSNLRDNKVNPEEQPISILFLGIDDNDGRRKKGQDAEHSRSDAMILTTFNQSKHQI
                     RMLSIPRDTISYIPKVGYYDKITHAHAYGGPIAAMDSVEATMNVPVDYYVRVNMKAFV
                     EAVNELGGIYYDVPYDLNEPNTDDTGKIKIKKGYQKLNGDEALAVARTRHHDSDLKRG
                     QRQMELIKILFQKAQEVDSIDKLDNVIQIVGKNAKHNLTNSEIKALAKMYLTNDVEIK
                     TAQLKGKDDMLNGIYYYHPSVESIQKYANLLRKDLELSPINDKNDFLDQRVINHYGSL
                     IPLTPLDSSLLRKEQNDTTDKDKTSNENNDSTNNSDSSNQQQPATDQNLNQNQGGTQQ
                     APQASNNQNGVVN"
BASE COUNT         2022 a         1142 c         1162 g         2601 t
ORIGIN      
        1 ataaacaact tgaaaaactt attacactga ctaacaatga cttaaactta gtgaatagaa
       61 gatttggaca acgcacggat atcacatctg aacagctaga acttctccgt attttattta
      121 attacgatcg cttatcacag tatgatttga cgatgaagat tagcagggaa caatctatag
      181 tttcaaggtg gattaagaaa ttagttttga aaggttacat cacaagtcaa caatctagcg
      241 aagatttaag atgtaaagaa ttaattttga ctgatcaagc acgtacatta atttcacaaa
      301 taaataatgc acgttgcgaa ttgattgaag caagatgtca atgtttatcg gaagtcgaat
      361 tagacaattt aaatcaatta cttgataagt taaatcaacg acgcatatcg ttgtaacacg
      421 acaatagcaa acacataatt tagtgtcatt gtgcacacaa caaaaattga atcatgaatt
      481 acaagcaaaa gtagcggtga ttgttaaaat tgatggtaaa caatcaccgc tatttttgct
      541 tgtgtatgta taaaaaaggg atcaaaggtc atcccccatg attgatagtg ggggatgact
      601 tttgatccta tgttcatgtt gcttatttat attgtgggat gtcgaagtat ttgccgactt
      661 cgccaatttt atcatagtag cctttgatga ttttagcatt gatgttagcc caatctacat
      721 ctgtagcata ttggtgtgtt cctggatgtg caggattcca tctcattttg taaagtgtat
      781 tttgaccagc ttttacatat gagttgccga tgaatttagc accaccaacg attgcttttg
      841 atactgtgtc ccaaccagct tgtttagcat atttaatacc ttcacgtaaa ggatcgttat
      901 catatgcagc aataccaaat acgttatggt atttcgtgtt tgagttagtt acaactttgt
      961 tgttcactac atctgcacct ttcgctaatt gagaagtacc gttacctgtt tctaataggg
     1021 catgtgagat aagataaact tcattaatgc catacatttg agcagctttg ttaaatgcag
     1081 caccttggtt ttctaataca cctttacctt ttaagaattg attaatttta tcaatagaaa
     1141 tattttgtgg ttggtctaag cgtaagaatt gatattttaa tgctggatct tgagctaaac
     1201 gcttcgtatc cattgcatgc ttaacatcat taaagttagc atctgtccac ttacctggta
     1261 cacgttgtac ttgtggttta tattgtaaac cagcttgtat ttgagcaact tggtttaatg
     1321 tcatacctgt ttgattatac ttaattaatt ctttagctaa atcagttgat ttaatccatg
     1381 ctaatttacc gttagataat ttaccatagt accaagtttg tccattaatg acttgttctt
     1441 taacaactgc gaatggttgt tcattaaatg cttttaatga gtatttagct gtatcagaat
     1501 ttggtgttac atagtaataa ccattaccat ttttaattac ataagtgtag ttataatctt
     1561 tggcagctga tgtagttggt ttcacagcag ttggtgcagt taaatctttt gcatttaccc
     1621 aaccagtgcg gttattaata gtaccgtata aataaacatc tttgcctaca gatacttgtt
     1681 tcgttgcatt aaatgtacct tgagcaatgt tattgcctgt taaaatgact tggtttttag
     1741 tactccaagg aaccattgat aagccgttat ttgatttatt aacagtatat ttttgagtcg
     1801 ttttaacttc tttgcctagg ttttgaacat ttaagtcttt tacattgaac caacctaatg
     1861 ggatgttatg gcttgtattg tttaataata catacgtttc attaccatga gcacgctctt
     1921 ttgttacata gaacgtacgg tctgcatatt tcgcaccgtt tttcgctgtt ttttcataaa
     1981 cagaagcacg aataccagtg ttgtttggtt taacttgagc aatcttgcta actgtttgag
     2041 tcgtttgtgg tttagtaaca gtataagctt ttacagctgt ttttggttgt gctactgctt
     2101 ttttaggtgc agcaggtaca gctaaatatg ctttacttac ccaaccagat ttaccattta
     2161 cagttccaaa taaatagata gatttatcaa tttgttgttg cttagtcgct ttaaaagttt
     2221 ggttacctgt accagaaact gcaccagctt cttgtttata agtgccccaa ggtactgaat
     2281 ataatttagt gcctggtttt actgtatatg tttgcattac atttacaggt gattttgcat
     2341 tgttataaat aacgtcacct tgtttaaccc aaccaattaa agttggacta ttgtaatctt
     2401 taactaagta gaatttgttt ccacctaaac ttgcttcttt tgttacagca aatgtttttt
     2461 gaacttcttt cgttggctta ccagttttgt cataaactgt agtgaataag ccattgtttt
     2521 tagcattaat ttgagcaaca ccgtttaatg atgaaactgt taatttatta tttgttgtag
     2581 gtgttgatgg cttaggtatt ggtgtaggcg taggtttagc agtatcaact aaatatgctt
     2641 tacttaccca accagattta ccattcacag agccatataa ataaattgat ttatcaattt
     2701 gttgttgctt tgaagcctta aatgtttggt ttccagagcc agacacgcta ccagcaactt
     2761 gtttagatgt accccaaggt actgtataaa gtttcgtacc agatttgatt gaatatgatt
     2821 gatttacatt tacaggtgat ttagctgtgt tgtaaaccac atcgccttct ttaacccaac
     2881 caaatttatt accagaattg taatcttgaa caagatagaa tttttgatta cctaatgtag
     2941 ctgttttaga tacagcaaat gttttttgaa cttcattagt tgctttacca gttttgtcgt
     3001 aaacagtagt atataaacca ctatttgttg gtttgatttg tgcgacacca ttgtttgctg
     3061 caactgttaa tttaccagtt gatggtttcg acggtgttgt tggttttgat ggtgtagtag
     3121 gggtagttgt aaattgcgta ccccatggcg ccactttacc catttttatt aaatattttt
     3181 cattaattaa gtcatataat tgatcataac tataattatg acttcttaaa tatccatgtg
     3241 gatcggcatg gtccgtacca cctaaatatt tacttacagc gtagtgagtc catactgtac
     3301 catttccatc atactcagca ctgtctggtt ttaaaccata atattgtaat tgtgtagctg
     3361 catagtcagc atagttattc attgaacgtg caaatgaagc atagtcgtgt gtgtgtacga
     3421 tttcaacatt gatgaatcta gggttaccga ctgcaccgac accccaagat aagtaatccg
     3481 ttggtgctgt ttcgattata cgatccccat caacaaatgc atgtacgaat gcgttttgat
     3541 agttattttt catataacta atttcaccat ttatcgtcga acgatcatta gctgtatcat
     3601 gaactacgat accttcagga cgacctacgc cgttacggta tgcgtattta gggaagtaag
     3661 atgtataatc ttcttcaatt ttaggtgctt ttaagttatt tttacgaatg taatcgttaa
     3721 ttgaagagtt tacttgtggt ttatattttg gcaaactcgt ttttggtgtt gcagcaactg
     3781 atcttggttg tgctgaagcg ctaaaagtag ttactttagg tgtcgcttca gttttagctt
     3841 taggtgctgc ggctttatat tgcgtttcaa gagctgcagg tttagcagct gatttaatta
     3901 attctggatt aatttgatgt tctgaattat catcttcatc atcaactaaa ctataaccag
     3961 catttgtaac attagtgtta gttttaggtg ctgtagtgct tgttgacttt gcaacaggct
     4021 gcgtattatt tgtagtcgct gattgattag cacgagtgtc accatttact tgtgcagtat
     4081 caactttttg acttacttga gcattgcctg ttttgttatt tgctgttttt ggttggacaa
     4141 tagcagggtc ttgatatact tgagtgccag aaatgttttg cgttggattt tttacctcag
     4201 cttttgcttg ttcagtagtt gctttaactt tattactatc taaaacattt ttattagtag
     4261 tttgatcttg tgtcgtctca gctgcttgaa cttgatgtgc agtgactgct gaacctacaa
     4321 gcgttaatgc aaccattgat ggtagtttgt aattgaattt tttcgccatt ctatttatta
     4381 ctcctaacat ttattaatta ttactaacat tatagtacct gttttatata cctgtgcgta
     4441 tttaaccaat ttgtaatata ctcttaattt acattttcac tgtaaaaaaa tgaaaaaaat
     4501 tttgaatctc aatggtgacg tgacaaatca aatagaattg atgtcaattt aagaattggt
     4561 ttattacaaa tgtttctaaa atagatacaa ctttttttaa ttaagcgagg ttaacttttt
     4621 tgtcatttca atatgctcga tgccttcctc aagaaatata ttacctctca ttttaaagtt
     4681 taaactttca taaaatggga tagcatgaca ttgggcattc atagtagcta cgtaaaaacc
     4741 ttcatcttta gctaatgatt ctacagcttg cataagcatt ctacccattc cttgtccacg
     4801 atgtgatttc atcacagcta ctcgttctat tttgacagtt gtttcattaa tagggcgtat
     4861 tcgagcagtg gcaactggct gtccattatc atatccaatg aggtgaatag attcagattc
     4921 atattcatca atttcacttt cctcagggac gccttgttct tctacaaaca ctttctttct
     4981 tatatagaag caatcttcta acatcttttg attgtttact tttgaaaaca taaaattcct
     5041 ccacatccta acaaaatcat ttacatttaa tttactaata ataacataga ataacgattg
     5101 ttattttcct caaaaataat tagacataaa aaagctataa agatgattca caagaatcat
     5161 ctttatagct aattaattat taatgttata gtgcttcttt tctaataatt gctgtatatt
     5221 gccaaaagat tcgcatttga gcaatattcc aattattcat tcccattgta aatcctgaag
     5281 gcttaaatat attcacatca gttacttcta atgctgctgc aactaaatat tgtaatggaa
     5341 tgcttagtga tttcattgtt tcggtaatgc cattttcatc atatacccat gaaaatggta
     5401 actctgattc gaaaacatca acactattga aaatttcagg tagggctaaa atataacatg
     5461 caccatctac aataggattg tcgatactat ttttgtaata aattaggagt gcttcatagt
     5521 tttctcgatg ctcatgattg acgtaaaaaa attcacttct aaaatgagcc atatcttggc
     5581 tatgaatgat ttgttgttct aacatattaa acatgccatt tatatttgca atcttttcgt
     5641 atgtttttct actcatgtat gatgctcctt tcataaatag taaatgttaa tcgtcatata
     5701 atattaattt acaacaccat tttggttatt tgaagcttgt ggcgcttgtt gtgtgccacc
     5761 ttgattttga tttaagtttt gatctgtagc aggttgttgt tgattgctgg aatcactgtt
     5821 attagttgaa tcattgttct cgttagatgt cttatcttta tctgtcgtat cattttgttc
     5881 ttttctcaat aaactactat ctaaaggcgt taacggtatt aatgaaccat aatgattaat
     5941 gacacgttga tctaagaaat catttttatc attaataggt gataattcta agtctttacg
     6001 aagtaagttt gcatattttt gaatgctttc aacacttgga tgataatagt aaataccatt
     6061 taacatatca tctttacctt ttaattgcgc agttttaatt tcaacatcat ttgttaagta
     6121 catttttgct aaagctttaa tttcagagtt agttaaatta tgctttgcat ttttgcctac
     6181 aatttgaatc acgttatcaa gtttatcaat agaatcaact tcctgtgctt tttggaataa
     6241 aatcttaatt aattccattt gacgttgtcc acgttttaag tctgaatcat gatgtctagt
     6301 tctagcaact gctaaagcct catcaccatt taatttttgg tacccttttt taattttaat
     6361 cttaccagta tcatctgtgt taggttcatt taagtcgtat ggcacatcat agtatatgcc
     6421 accaagctca tttacagcct cgacaaatgc tttcatattg actctcacat aataatcaac
     6481 aggtacattc atggtagctt ctaccgaatc cattgcggca attggaccac catatgcatg
     6541 tgcatgggta atcttatcgt aatagccaac tttaggaatg tagctgatag tatcacgtgg
     6601 aatactaagc attctaattt gatgttttga ttgattaaaa gtagttaaaa tcatagcgtc
     6661 tgatctagag tgttcagcat cctgtccttt ttttcttctt ccatcgttat catcgatacc
     6721 taagaaaaga atagagatag gttgttcttc gggattgact ttattatctc ttaagttgga
     6781 ttgacgatta gcatttttgc tgtcttgaga agattcgaat gcatcttggg acgttttaaa
     6841 aagtaacgta gcgaagacta ttggaacaac aatgagaacc aatgctagaa ggatcaaaaa
     6901 gtattttaaa aatttattca tgattga
//
LOCUS       DANBWH010000146         6712 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.150, whole genome shotgun sequence.
ACCESSION   DANBWH010000146 DANBWH010000000
VERSION     DANBWH010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6712
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.150"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            19..714
                     /locus_tag="PPY92_002025"
     CDS             19..714
                     /locus_tag="PPY92_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL7"
                     /protein_id="HDJ2626011.1"
                     /translation="MKLKTLAKATLALGLLTTGVITSEGQAVHAKEKQERVQHLYDIK
                     DLYRYYSSESFEFSNISGKVENYNGSNVVRFNQEKQNHQLFLLGKDKDKYKKGLEGQN
                     VFVVKELIDPNGRLSTVGGVTKKNNKSSETNTHLFVNKVYGGNLDASIDSFLINKEEV
                     SLKELDFKIRKQLVEKYGLYKGTTKYGKITINLKDEKKEVIDLGDKLQFERMGDVLNS
                     KDIQNIAVTINQI"
     gene            1053..1751
                     /locus_tag="PPY92_002026"
     CDS             1053..1751
                     /locus_tag="PPY92_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL8"
                     /protein_id="HDJ2626012.1"
                     /translation="MKFTAIAKAIFVLGILTTSVMITENQSVNAKGKYEKMNRLYDTN
                     KLHQYYSGPSYELTNVSGQSQGYYDSNVLLFNQQNQKFQVFLLGKDENKYKEKTHGLD
                     VFAVPELVDLDGRIFSVSGVTKKNVKSIFESLRTPNLLVKKIDDKDGFSIDEFFFIQK
                     EEVSLKELDFKIRKLLIKKYKLYEGSADKGRIVINMKDENKYEIDLSDKLDFERMADV
                     INSEQIKNIEVNLK"
     gene            2128..2826
                     /locus_tag="PPY92_002027"
     CDS             2128..2826
                     /locus_tag="PPY92_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL9"
                     /protein_id="HDJ2626013.1"
                     /translation="MKLTALAKATLALGILTTGVFTAESKAVHAKVELDETQRKYYIN
                     MLHQYYSEESFESTNISVKSEDYYGSNVLNFNQRNKTFKVFLLGDDKNKYKEKTHGLD
                     VFAVPELIDIKGGIYSVGGITKKNVRSVFGFVSNPSLQVKKVDAKHGFSINELFFIQK
                     EEVSLKELDFKIRKMLVEKYRLYKGASDKGRIVINMKDEKKYVIDLSEKLSFDRMFDV
                     MDSKQIKNIEVNLN"
     gene            3192..3875
                     /locus_tag="PPY92_002028"
     CDS             3192..3875
                     /locus_tag="PPY92_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL10"
                     /protein_id="HDJ2626014.1"
                     /translation="MKFTALAKATLALGILTTGTLTTEVHSGHAKQNQKSVNKHDKEA
                     LYRYYTGKTMEMKNISALKHGKNNLRFKFRGIKIQVLLPGNDKSKFQQRSYEGLDVFF
                     VQEKRDKHDIFYTVGGVIQNNKTSGVVSAPILNISKEKGEDAFVKGYPYYIKKEKITL
                     KELDYKLRKHLIEKYGLYKTISKDGRVKISLKDGSFYNLDLRSKLKFKYMGEVIESKQ
                     IKDIEVNLK"
     gene            complement(3960..4061)
                     /locus_tag="PPY92_002029"
     CDS             complement(3960..4061)
                     /locus_tag="PPY92_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626015.1"
                     /translation="MENFDVGGGIFIFMEIGIGFMTHKQKRLNDDID"
     gene            4139..5695
                     /locus_tag="PPY92_002030"
     CDS             4139..5695
                     /locus_tag="PPY92_002030"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDJ2626016.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIR
                     EIETQDFDIEHLVTAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVT
                     DGKDKLRHVYDPTCGSGSLLLRVGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYEN
                     FDIRNDDTLENPAFLGNTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADF
                     AFIQHMVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPVNIFYG
                     TSIPTCILVFKKCRQQDDNVLFIDASNDFEKGKNQNHLSDAQVERIIDTYKRKETIDK
                     YSYSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAY
                     LKELGVLKDE"
     gene            5688..>6712
                     /locus_tag="PPY92_002031"
     CDS             5688..>6712
                     /locus_tag="PPY92_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDJ2626017.1"
                     /translation="MSNTQKKNVPELRFPGFEGEWEEKQLGDLTDRVIRKNKNLESKK
                     PLTISGQLGLIDQTEYFSKSVSSKNLENYTLIKNGEFAYNKSYSNGYPLGAIKRLTRY
                     DSGVLSSLYICFSIKSEMSKDFMEAYFDSTHWYREVSGIAVEGARNHGLLNVSVNDFF
                     TILIKYPSLEEQQKIGKFFSKLDRQIELEEQKLELLQQQKKGYMQKIFSQELRFKDEN
                     GEDYPDWENSKIEKYLKERNERSDKGQMLSVTINSGIIKFSELDRKDNSSKDKSNYKV
                     VRKNDIAYNSMRMWQGASGKSNYNGIVSPAYTVLYPTQNTSSLFIGYKFKTHRMIHKF
                     KINSQGLT"
BASE COUNT         2633 a          880 c         1232 g         1967 t
ORIGIN      
        1 gattgggaga atagtactat gaaattaaaa acgttagcta aagcaacatt ggcattaggc
       61 ttattaacta ctggtgtgat tacatcagaa ggccaagcag tccacgcaaa agaaaagcaa
      121 gagagagtac aacatttata tgatattaaa gacttatatc gatactactc atcagaaagt
      181 tttgaattca gtaatattag tggtaaggtt gaaaactata acggttctaa cgttgtacgc
      241 tttaaccaag aaaaacaaaa tcaccaatta ttcttattag gaaaagataa agataaatat
      301 aaaaaaggcc ttgaaggcca gaatgtcttt gtggtaaaag aattaattga tccaaacggt
      361 agactatcta ctgttggtgg tgtgactaag aaaaataaca aatcttctga aactaataca
      421 catttatttg ttaataaagt gtatggcgga aatttagatg catcaattga ctcattttta
      481 attaataaag aagaagtttc actgaaagaa cttgatttca aaattagaaa gcaattagtt
      541 gaaaaatatg gtttatataa aggtacgact aaatacggta agatcactat caatttgaaa
      601 gacgagaaaa aggaagtaat tgatttaggt gataaactgc aattcgagcg catgggtgat
      661 gtgttgaata gtaaggatat tcaaaatata gcagtgacta ttaatcaaat ttaaattaaa
      721 caatcaatga ctttaaagta ataaatttga agcagcttaa cgatgaaatg ttgaataaat
      781 acgtacatcc tccaaaaagg ggcgtatcta aatcaacagt gtcgttaggc tgtttttatg
      841 ttttataacg cagggtatga gcgtactaaa aattcacatt acttctgaaa gtgatgtcca
      901 ttgaatatta attagttctt cattaaccat gatttaattt taattaaacg agtgttaatg
      961 tcagtctgtc tcaatgccct ttataataaa tgtgtattat tcaaattacg taataaaagc
     1021 aatccaatat attaagattg gagcatatga atatgaaatt tacagcgata gctaaagcga
     1081 tatttgtatt aggaatatta acaacaagtg taatgataac agaaaatcaa tcggttaatg
     1141 caaaaggaaa gtatgaaaaa atgaaccgtt tatatgatac aaacaagtta catcaatact
     1201 attcaggacc tagttatgag ttaacaaatg ttagtggcca aagtcaaggt tattatgact
     1261 ctaacgtttt gctttttaac caacaaaatc aaaagttcca agtattttta ttgggaaaag
     1321 atgaaaataa atacaaagaa aaaacacatg gtttagatgt ctttgcggta ccagaattag
     1381 tagatttaga tggaagaata tttagtgtta gtggtgtaac gaagaaaaac gtaaaatcaa
     1441 tatttgagtc tctaagaacg ccgaacttac tagttaaaaa aatagacgat aaagacggtt
     1501 tttctattga tgaatttttc tttattcaaa aggaagaagt gtcattgaag gaacttgatt
     1561 ttaaaataag aaaactgttg attaaaaaat acaaactgta tgaagggtca gctgataaag
     1621 gtagaattgt tattaatatg aaagatgaaa ataagtatga aattgattta agtgataaat
     1681 tagatttcga gcgtatggca gatgtcatta atagtgaaca aattaagaac atcgaagtga
     1741 atttgaaata atcaatgata tatagaataa aagcttaaga agcggtttaa taatcccatg
     1801 tttaatgatt ttgatacgtg ttttaataat aaaaacatat cgaacattga ctacgttatt
     1861 aagctgcttt tttgtacact ttgtatcgaa taacttaaga tctaaaacta atcggaaaga
     1921 acaatgattc ccccaaaaaa atttatgttg ctattaaaaa tcagttaata cgaatgttaa
     1981 aatacgtttg attttcatta ataatgattc aagtttattt aaatgagcgt taatgtcagt
     2041 ctgttttgat gcaccttata ataaagacag atagttcaaa ttacgtaatc ataacaatcc
     2101 aatatatcaa gattggagca aataaatatg aaattgactg cattagcaaa agcaacatta
     2161 gcattaggaa tattaactac aggtgtgttt acagcagaaa gtaaagctgt tcacgcgaaa
     2221 gtagaacttg atgagacaca acgcaaatat tatatcaata tgctacatca atactattct
     2281 gaagaaagtt ttgaatcaac aaacattagc gttaaaagtg aagattacta tggttctaac
     2341 gttttaaact ttaaccaacg aaataaaact ttcaaagtgt ttctgcttgg tgacgataaa
     2401 aataaatata aagaaaaaac acatggcctt gatgtctttg cagtacctga attaatagat
     2461 ataaaaggtg gcatatatag cgttggcggt ataacaaaga aaaatgtgag atcagtgttt
     2521 ggatttgtaa gtaatccaag tctacaagtt aaaaaagttg atgctaaaca tggcttttcg
     2581 ataaatgagt tgttctttat tcaaaaggaa gaagtatcgt tgaaggaact ggattttaaa
     2641 ataagaaaaa tgttagtcga aaaatataga ttgtataaag gcgcgtcaga taaaggtaga
     2701 atcgttatta atatgaaaga cgaaaagaaa tatgtaattg atttaagtga aaaattaagt
     2761 tttgatcgta tgtttgatgt aatggatagt aagcaaatta aaaatattga agtgaatttg
     2821 aattagtttg agttaatagc ataatagctt aagaagcgac ttaacgacaa aatgtgaatt
     2881 gacatctgtg tccttatata aggaactgtg ttaaatacat tactgttgtt aagttgtttt
     2941 tgtaattcaa agagcagaac agagtaacat catcagttgt agtaaacgat aatccagtaa
     3001 aacaactaaa tgaaataatg aaagtcattt aacctgaaca ttaaaatata tttgtttttc
     3061 attaagaata attcaagtat atttaaatcg aggttaatta tcgtatgaaa cgatgcacgt
     3121 tataataaaa atgtatgatt caaattacgt aatgaaaaca atccaatata ttaagattgg
     3181 agcaaataaa tatgaaattt acagcattag caaaagcaac attagcttta ggaattttaa
     3241 caacaggaac tttaacaaca gaagttcatt caggtcatgc aaaacaaaat caaaagtcag
     3301 taaataaaca tgacaaggag gcactatacc gatactacac tggaaagact atggaaatga
     3361 aaaatattag tgctttgaaa catggtaaaa ataacttgcg ttttaagttt agaggtatta
     3421 agattcaagt tttactgcct ggaaatgata aaagtaaatt tcaacagcgt agttatgagg
     3481 ggttagatgt gttttttgtt caagaaaaaa gagataagca cgatatattt tatactgttg
     3541 gtggtgtaat acagaataat aaaacatctg gagttgtcag tgcaccaata ttaaatattt
     3601 caaaagaaaa gggtgaagat gcttttgtga aaggttaccc ttattacatt aaaaaagaaa
     3661 aaataacact aaaagagctg gattataagt tgagaaagca tctaattgaa aaatacggac
     3721 tttataaaac aatctcaaaa gatggtaggg tcaaaattag cttgaaagat ggcagttttt
     3781 ataaccttga tttaagatct aaattaaaat ttaaatatat gggggaagtc atagaaagca
     3841 aacaaattaa agatattgaa gttaacttaa agtaaataat tacgaataat aaaaagtaat
     3901 tgaagcggct taacggtgaa atgtaaattg gtgcgcatag cttatacaaa aaggatgcat
     3961 caatcgatat cgtcgttaag ccgtttttgt ttgtgtgtca tgaatcctat cccaatctcc
     4021 atgaatataa aaattccacc accaacatca aaattctcca catcgcaaca taaccaaatg
     4081 ttataataaa tctattacac aaagagataa attacttatt caaaggcgga ggaatcacat
     4141 gtctattact gaaaaacaac gtcagcaaca agctgaatta cataaaaaat tatggtcgat
     4201 tgcgaatgat ttaagaggga atatggatgc gagtgaattc cgtaattaca ttttaggctt
     4261 gattttctat cgcttcttat ctgaaaaagc ggaacaagaa tatgcagatg ccttgtcagg
     4321 tgaagatatt acgtatcaag aagcatgggc agatgaagaa tatcgtgaag acttaaaagc
     4381 tgaattaatt gatcaagtcg gttacttcat tgaaccacaa gatttattca gtgcgatgat
     4441 tcgtgaaatt gaaacgcaag atttcgatat cgaacatctc gtaacggcga ttcgtaaagt
     4501 tgaaacatca acactaggtg aagaaagtga aaatgacttt atcggactgt tcagcgatat
     4561 ggacttaagt tcaacgcgac taggtaacaa tgtcaaagaa cgtactgcac taatttccaa
     4621 agttatggtt aaccttgacg acttaccatt cgttcacagt gatatggaaa ttgatatgtt
     4681 aggtgatgca tacgaatttc ttatcgggcg ctttgcggcg acagcaggta aaaaagcagg
     4741 cgagttctat acaccacaac aagtatctaa gatactggcg aagattgtca cagacggtaa
     4801 agataaatta cgtcacgtgt atgacccaac atgtggttca ggttcattat tgttacgtgt
     4861 tggtaaagaa acgcaagtgt atcgttattt cggtcaagaa cgtaacaata ccacttacaa
     4921 cttagcgcgc atgaacatgt tattacatga tgtgcgctat gaaaactttg atatccgtaa
     4981 tgatgatacg ttggaaaatc cagctttctt aggcaataca tttgatgcgg ttatagcgaa
     5041 cccaccatac agtgcgaaat ggactgcaga ttcaaaattt gaaaatgatg aacgcttcag
     5101 tggttacggc aagcttgcgc caaaatccaa agcagacttt gcctttattc aacatatggt
     5161 acattaccta gacgatgaag gtaccatggc agttgtactc ccacatggtg tcttattccg
     5221 tggtgccgca gaaggcgtca ttcgtcgtta tttaattgaa gaaaagaact acttagaagc
     5281 cgtgattggc ttaccagtga atattttcta tgggacaagt attccaacat gtatcttagt
     5341 atttaaaaaa tgtcgccaac aagacgacaa cgtattattt atcgatgcat ccaatgattt
     5401 tgaaaaagga aaaaatcaaa accatttaag cgatgcccaa gtcgaacgta ttattgacac
     5461 atacaagcgt aaagaaacaa ttgataaata cagttacagt gcgacattac aagagattgc
     5521 cgataacgat tacaacctaa acataccgag gtatgtcgat acattcgaag aagaagcgcc
     5581 aattgattta gatcaagtcc aacaagattt gaaaaatatc gacaaagaaa tcgcagaaat
     5641 tgaacaagaa atcaatgcat acctgaaaga acttggggtg ttgaaagatg agtaatacac
     5701 aaaagaaaaa tgtgccagag ttgagattcc cagggtttga aggcgaatgg gaagagaagc
     5761 agttggggga tcttacagat agagtaatta ggaaaaataa aaacttagaa tcgaaaaagc
     5821 ctttaacaat atccggacag ttaggtttaa ttgatcaaac agaatatttt agtaaatcag
     5881 tttcgtcgaa aaatctagaa aattatacac taataaagaa tggagaattc gcgtataaca
     5941 aaagttattc taatggatac ccattagggg ctattaaaag attaactaga tatgatagtg
     6001 gtgtattgtc ctctttgtat atttgttttt ctattaaaag tgaaatgtct aaagacttca
     6061 tggaagcata ttttgattcg acacactggt atagagaagt ttctggaatt gcagttgagg
     6121 gtgcaagaaa tcacggatta ttaaatgttt ctgtgaatga tttttttact attctaatta
     6181 aatatccaag tttagaagaa cagcaaaaaa taggcaagtt cttcagcaaa ctcgaccgac
     6241 aaattgaatt agaagaacaa aagcttgaat tacttcaaca acagaaaaaa ggctatatgc
     6301 agaaaatttt ctcacaggaa ctgcgattca aagatgagaa tggtgaagat tatccagatt
     6361 gggaaaatag caaaatagaa aaatatttaa aagagagaaa cgaacgttct gacaaagggc
     6421 aaatgctttc agtaactata aatagtggca ttataaaatt tagtgaattg gatagaaaag
     6481 ataattcaag taaagataaa agtaattata aagtagttag gaaaaatgat attgcatata
     6541 attctatgag aatgtggcaa ggggctagtg gtaaatcaaa ttataatggg attgttagcc
     6601 ctgcatatac tgtgctttat ccaacacaaa atactagctc attatttatt ggatataagt
     6661 ttaaaacaca tagaatgatt cataaattta aaattaattc acaaggatta ac
//
LOCUS       DANBWH010000147         6670 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.152, whole genome shotgun sequence.
ACCESSION   DANBWH010000147 DANBWH010000000
VERSION     DANBWH010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6670
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.152"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(68..670)
                     /gene="rpsD"
                     /locus_tag="PPY92_002032"
     CDS             complement(68..670)
                     /gene="rpsD"
                     /locus_tag="PPY92_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019165370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDJ2626018.1"
                     /translation="MARFRGSNWKKSRRLGISLSGTGKELEKRPYAPGQHGPNQRKKL
                     SEYGLQLREKQKLRYLYGMTERQFRNTFDIAGKKFGVHGENFMILLASRLDAVVYSLG
                     LARTRRQARQLVNHGHILVDGKRVDIPSYSVKPGQTISVREKSQKLNIIVESVEINNF
                     VPEYLNFDADSLTGTFVRLPERSELPAEINEQLIVEYYSR"
     gene            complement(914..1378)
                     /locus_tag="PPY92_002033"
     CDS             complement(914..1378)
                     /locus_tag="PPY92_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HDJ2626019.1"
                     /translation="MTTINPTNYTLLKKQAASLIEDEHHMIAILSNMSALLNDNLNQI
                     NWVGFYLLEQNELILGPFQGHPACVHIPIGKGVCGTAVSERRTQVVADVHQFEGHIAC
                     DANSKSEIVVPIFKDDKIIGVLDIDAPITDRFDDNDKEHLEAIVKIIEKQLA"
     gene            1515..3209
                     /gene="ezrA"
                     /locus_tag="PPY92_002034"
     CDS             1515..3209
                     /gene="ezrA"
                     /locus_tag="PPY92_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HDJ2626020.1"
                     /translation="MVLYIILAIIVIILIAVGVLFYLRSNKRQIIEKAIERKNEIETL
                     PFDQNLAQLSKLNLKGETKTKYDAMKKDNVESTNKYLAPVEEKIHNAEALLDKFSFNA
                     SQSEIDDANELMDSYEQSYQQQLEDVNEIIALYKDNDELYDKCKVDYREMKRDVLANR
                     HQFGEAASLLETEIEKFEPRLEQYEVLKADGNYVQAHNHIAALNEQMKQLRSYMEEIP
                     ELIRETQKELPGQFQDLKYGCRDLKVEGYDLDHVKVDSTLQSLKTELSFVEPLISRLE
                     LEEANDKLANINDKLDDMYDLIEHEVKAKNDVEETKDIITDNLFKAKDMNYTLQTEIE
                     YVRENYYINESDAQSVRQFENEIQSLISVYDDILKEMSKSAVRYSEVQDNLQYLEDHV
                     TVINDKQEKLQNHLIQLREDEAEAEDNLLRVQSKKEEVYRRLLASNLTSVPERFIIMK
                     NEIDHEVRDVNEQFSERPIHVKQLKDKVSKIVIQMNTFEDEANDVLVNAVYAEKLIQY
                     GNRYRKDYSNVDKSLNEAERLFKNNRYKRAIEIAEQVLESVEPGVTKHIEEEVIKQ"
     gene            complement(3283..3425)
                     /locus_tag="PPY92_002035"
                     /pseudo
     CDS             complement(3283..3425)
                     /locus_tag="PPY92_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500331.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3574..4722
                     /locus_tag="PPY92_002036"
     CDS             3574..4722
                     /locus_tag="PPY92_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDJ2626021.1"
                     /translation="MFILIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQA
                     NQLLQQAKAQINAMINSKTNYDVVFTSGATESNNLALKGIAYRKFDTAKEIITSVLEH
                     PSVLEVVRYLEAHEGFKVKYVDVKKDGSINLEHFKELVSDKVGLVTCMYVNNVTGQIQ
                     PIPQMAKVIKNYPKAHFHVDAVQAFGKISMDLNNIDSISLSGHKFNGLKGQGVLLVNH
                     IQNVEPTVHGGGQEYGVRSGTVNLPNDIAMVKAMKIANENFEALNAFVTELNNDVRQF
                     LNKYHGVYINSSTSGSPFVLNISFPGVKGEVLVNAFSKYDIMISTTSACSSKRNKLNE
                     VLAAMGLSDKSIEGSIRLSFGATTTKEDIARFKEIFIIIYEEIKELLK"
     gene            4722..5945
                     /gene="thiI"
                     /locus_tag="PPY92_002037"
     CDS             4722..5945
                     /gene="thiI"
                     /locus_tag="PPY92_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDJ2626022.1"
                     /translation="MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVK
                     GKRDRMYIELEDHADINEITYRLSKIFGIKSISPVLKVEKTIEAMSAAAIKFAQQFEE
                     NSTFKIDVKRADKNFPMDTYELQRELGGTVLKQIENVSVNVKRPDHEIRVEVRLDAIY
                     MYEEVVPGSGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQ
                     AKEKVIELTRILAERVGPIKLHIVPFTELQKQVNKVVHPRYTMTSTRRMMMRVADKLV
                     HQIGALAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYDKEEIIIKSKEIGTF
                     ETSIQPFEDCCTIFTPKNPVTEPNFEKVVQYESVFDFEEMINRAVENIETLEITSDYK
                     TIKEQQTNQLINDFL"
     gene            5989..>6670
                     /locus_tag="PPY92_002038"
     CDS             5989..>6670
                     /locus_tag="PPY92_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDJ2626023.1"
                     /translation="MDLNLTMIIIIILFGFIAAFIDSVVGGGGLISTPALLAIGLPPS
                     VALGTNKLASSFGSLTSMIKFIRSGKVDLFVVAKLFGFVFLASACGAYIATMVPSQIL
                     KPLIIIALSSVFIFTLLKKDWGNTRTFTQFTFKKAIIFAALFILIGFYDGFVGGGTGS
                     FMLFVLLVFGFDFLSAAGNAKVLNFASNIGALVLFMVLGQVDYVIGLIMAISMIAGSY
                     TGAHFAIKQ"
BASE COUNT         2385 a          909 c         1228 g         2148 t
ORIGIN      
        1 tttgaatcta tcatttttaa attaataaaa aaaacaccca caattgtggg tgtttgtatt
       61 gaacgtatta tcttgagtag tactcaacga ttaattgttc gttaatttca gcaggtaatt
      121 cgctacgttc tggtaaacgt acgaaagtac cagttaagct gtcagcatca aagtttaagt
      181 actcaggtac gaaattgttg atttcaactg attcaacgat gatgtttaat ttttgagatt
      241 tttcacgaac tgaaattgtt tgaccaggtt taacagaata agatggaata tcaacacgtt
      301 taccatctac taagatatga ccgtggttaa ctaattgacg tgcttgacga cgagtacgag
      361 ctaaacctaa tgaataaaca acagcgtcta aacgacttgc taataaaatc atgaagtttt
      421 caccgtgtac accgaatttt ttaccagcga tgtcaaatgt gttacggaat tgtctttcag
      481 tcattccata taagtaacgt aatttttgtt tttcacgtaa ttgtaaacca tattctgata
      541 attttttacg ttggtttgga ccatgttgtc ctggtgcgta aggacgtttt tctaattctt
      601 taccagtacc gcttaaagag atacctaaac gacgagattt tttccagttt gaacctctga
      661 atcgagccat aataagactc ctcctttaat tcttttttgt tgttatgaat aaacaaaaaa
      721 gagtgttgca tgcttactaa gatatattgt tttatgtgtc ttcaccttat agcgacagtt
      781 acgtgacacg tccgcattgg gaacaacata gcgctcaata atatacaact gctactttcg
      841 ttaattcaca caaagattat tgtaacattt tcctgctaac tgtcaacact ttattgaaaa
      901 tgctgatgtc cttttatgcg agttgctttt caataatttt aacaattgct tcaagatgtt
      961 ctttgtcatt gtcatcaaat cgatccgtta taggggcatc gatatctaag acgccgataa
     1021 ttttatcatc tttgaaaatt ggaacgacaa tctcagactt actattagca tcacaagcga
     1081 tatgtccttc gaattgatgg acatcagcta caacttgtgt acgacgttct gaaacggctg
     1141 taccgcatac accttttcca attggaatgt ggacacaagc ggggtgtcct tggaaaggtc
     1201 caagtataag ttcattttgt tccaataagt aaaagccaac ccaattaatt tgatttagat
     1261 tatcatttaa taaggcagac atgttactta aaatcgcaat catatgatgt tcatcttcaa
     1321 taaggcttgc tgcttgtttc tttaataatg tgtagtttgt tgggttaatt gttgtcatat
     1381 ttaaaacctc ttattttcat agattttata atagtataga atttcgaaaa tattatggca
     1441 ataaattcat tttataattt atccttaagc aaaattacgt tataatagta atgataaatt
     1501 aggaggagaa gcatatggtg ttatatatca ttttggcaat aattgtgatt atattgattg
     1561 ctgtaggtgt attattctat ttacgttcaa ataaacgaca aattattgaa aaagcaatcg
     1621 aacgtaaaaa tgaaattgag acgttacctt ttgatcaaaa ccttgcacaa ttatctaagt
     1681 tgaatttaaa aggtgaaaca aaaacgaaat acgatgcaat gaaaaaggac aacgtagaaa
     1741 gtacaaataa gtatctagct cctgtggaag aaaaaatcca taatgctgag gctttattag
     1801 ataaatttag tttcaacgca tctcaaagtg aaattgatga tgcaaatgag ttgatggata
     1861 gttacgaaca aagctatcag caacaattag aagatgtaaa tgaaattatt gcgttataca
     1921 aagataatga tgaattatat gacaaatgta aggttgatta tcgtgaaatg aaacgtgatg
     1981 ttttagcaaa tcgtcatcaa tttggtgagg cagcaagtct acttgaaact gaaattgaaa
     2041 agtttgagcc aaggttagag caatatgaag tactaaaagc tgatggtaat tatgtacaag
     2101 cgcacaacca tatagctgcc ttgaatgaac aaatgaaaca gctaagatct tatatggaag
     2161 aaataccaga attaattaga gaaactcaaa aagaattacc tggtcaattc caagatttaa
     2221 aatatggttg ccgtgatctt aaagttgaag ggtatgatct ggatcacgta aaagtagaca
     2281 gtacattaca aagcttaaaa acagagctta gtttcgttga accattaatt agccgcttag
     2341 aattagaaga agctaatgat aaactagcta atatcaatga taagttagat gacatgtatg
     2401 atttaattga acatgaagtt aaagctaaaa atgatgtcga agaaacaaaa gatatcatta
     2461 cggataactt attcaaagct aaagacatga attatacatt gcaaacagaa attgaatatg
     2521 tacgtgaaaa ctactatata aatgaatctg atgctcaaag tgttcgtcaa tttgaaaatg
     2581 aaattcaaag tttaatttct gtatatgatg atattttaaa agaaatgtct aaatctgctg
     2641 tacgatatag cgaggttcag gataatttac aatatttaga agatcatgtc acagttatta
     2701 atgacaaaca agaaaagcta caaaatcatc tgattcaatt gcgtgaagat gaagcagaag
     2761 cagaagacaa tctgctacga gtacaatcga agaaagaaga agtgtatcgt cgattacttg
     2821 cttctaactt aacaagcgtt cctgaaaggt ttatcatcat gaaaaatgaa attgatcatg
     2881 aagttcgtga tgttaacgaa caatttagtg aacgtccaat acacgttaaa cagttaaaag
     2941 ataaagtgtc taaaattgtg attcaaatga atacatttga agatgaagca aatgatgttc
     3001 ttgttaatgc tgtttatgca gagaaattaa ttcaatatgg aaatagatat cgtaaggact
     3061 atagcaatgt tgataagagc ttaaatgaag ctgaacgatt atttaaaaat aatcgctata
     3121 agcgtgcgat tgaaattgca gagcaagttc ttgaaagtgt tgagccaggt gtcactaaac
     3181 atattgaaga agaagttatt aagcaataga aactagtatg tagttatact taaataatat
     3241 gagcactctg tcaaattgga ctgatgagtt taataattga agttagccaa cgatacgttg
     3301 tctagcttct tttttttata tggataaatg aaagggacaa taaatataaa tagcaattgt
     3361 ttaaagataa acgtaatcaa atgtgttgtt ttaattaata taagtagtga aaaaagcata
     3421 atcacacagc tgtttaaata gagtgaaata gtctaattct tatttaatat gtagaaataa
     3481 gattattttg cgctataacc tttatttttt gtcggataat agtataatgc ttgagtaaaa
     3541 caattttaac gattgatgat taatgaaaga gaagtgttta ttttgatata tctagataat
     3601 gcggcaacga cgaaagcatt tgaagaagtg ttagatactt atttaaaagt aaatcaatca
     3661 atgtattata atccgaatag tccgcataaa gctggtttgc aggcaaatca attactacaa
     3721 caagcaaaag cccaaattaa tgcaatgatt aattcaaaaa caaattatga tgttgtattc
     3781 actagtggtg caactgaatc caataatctt gctttaaaag gtattgccta tcgtaaattt
     3841 gatacagcga aggaaataat tacatccgtg ttagagcatc cgtccgtatt agaggttgta
     3901 agatatttgg aagcacacga aggatttaaa gttaaatatg ttgatgtaaa gaaagatggc
     3961 agtattaact tagaacactt caaagaatta gtgtcagaca aagtcggttt agtaacatgt
     4021 atgtatgtaa ataatgtaac tggacaaata cagcctattc cacaaatggc taaagttata
     4081 aaaaattatc ctaaggcaca ttttcatgta gatgcggttc aagcattcgg caaaatttca
     4141 atggatctca ataacataga tagtattagt ttaagtggac acaagtttaa tggtttaaaa
     4201 ggacaaggcg tcttacttgt aaatcacatt caaaatgttg aaccaactgt ccatggtggt
     4261 ggtcaagaat atggcgttag aagtggaaca gttaatttgc caaatgatat tgcaatggtt
     4321 aaagcgatga agatagctaa tgaaaacttt gaagcattga atgcatttgt tactgagtta
     4381 aataatgacg tccgtcaatt tttaaataaa tatcatggag tttatattaa ttcttcaact
     4441 tcaggttcac cattcgtttt aaatattagt tttcctggcg taaaaggtga agtattagtt
     4501 aatgcttttt caaaatatga cattatgata tctacgacga gtgcttgttc atctaaacgt
     4561 aataaattaa atgaagtatt ggctgcaatg ggattatcag acaaatctat tgaaggtagt
     4621 ataagattat catttggggc tactacaact aaagaagata tagcaaggtt taaagaaata
     4681 tttatcatca tttatgagga aattaaggag ttgctaaaat aatgaagtat gatcacttgc
     4741 ttgttagata cggggagtta acattaaagg gttcaaatag aaaaaaattt gtaaatcaat
     4801 taagaaataa tgtaaataag tcattaaaag gacttgatgg gtttgtcgtt aaaggcaaac
     4861 gagatcgtat gtatattgaa cttgaagacc atgcagatat aaatgaaata acatatcgat
     4921 tatcaaaaat tttcggtatt aaatctatta gtccagtatt aaaagtagaa aaaacaatag
     4981 aggcaatgag tgcagcggca attaaatttg cgcagcaatt tgaagaaaac agcacattta
     5041 aaattgatgt gaagcgtgcc gataaaaatt tcccaatgga tacgtatgaa ttacaacgtg
     5101 aattgggtgg taccgtattg aaacaaattg aaaatgtttc tgttaatgtc aaacgtccag
     5161 atcatgaaat tcgagtggaa gttagattag atgcaattta tatgtatgaa gaagttgttc
     5221 cgggttcagg cggattacca gttggtactg gtggtaagac gttactaatg ctttcaggcg
     5281 gtatagactc accagttgct ggaatggaag tgatgagacg tggcgtaaca attgaagcga
     5341 ttcatttcca tagtccacca tttacaagtg atcaagcaaa agaaaaagtt attgaattga
     5401 cacgtatttt agctgaacgt gttggaccaa ttaaattgca tattgtacca tttacagaat
     5461 tgcaaaaaca ggtaaataaa gttgtacatc caagatatac aatgacttca acgagacgta
     5521 tgatgatgcg tgttgctgat aaattagtac atcaaatagg ggctttagct attgtaaatg
     5581 gtgaaaacct agggcaggta gccagtcaaa cacttcatag catgtatgca attaataatg
     5641 taacttctac tcctgtatta cgtcctttat taacttacga taaagaagaa attattatta
     5701 aatcgaaaga aattggtaca tttgaaacat ctattcaacc atttgaagat tgttgtacaa
     5761 ttttcacccc taaaaatcca gtaaccgaac caaactttga aaaggtagtc caatatgaaa
     5821 gtgtctttga ttttgaagag atgattaatc gtgctgttga aaatattgaa acacttgaaa
     5881 taactagtga ttataaaact attaaagaac agcaaacaaa ccaattaata aatgactttt
     5941 tataaataaa atcctagagt aaatttaaac ataaggggat gttaaactat ggatttgaac
     6001 ttaacgatga ttataatcat aattttattt ggttttatcg cggcgtttat agattcggtt
     6061 gtagggggtg gcggtttaat ttctacgcca gcattattag caatcggtct accaccatct
     6121 gtggctttag gtacaaataa attggcaagt tcatttggtt ctttaactag tatgataaag
     6181 tttataaggt ccggtaaagt ggacttattt gttgttgcca aattatttgg ttttgtattt
     6241 ttggcatctg catgtggcgc atatattgca acgatggttc cgtcacaaat attgaaacct
     6301 ctaatcatta ttgcactttc gtcggtgttt atattcacat tacttaaaaa agattggggc
     6361 aatacacgca cgtttactca atttacattt aagaaagcca taatatttgc agcacttttt
     6421 atattaatcg gcttttatga tggatttgta ggtgggggaa caggttcatt tatgcttttt
     6481 gtattgttag tctttggttt tgatttttta agtgcagcag gaaatgctaa agttttgaac
     6541 tttgcttcta atataggtgc gcttgtatta tttatggtat taggacaagt agattatgta
     6601 ataggtttaa ttatggctat aagtatgatt gctgggtcat atactggagc acattttgct
     6661 atcaaacaag
//
LOCUS       DANBWH010000148         6632 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.153, whole genome shotgun sequence.
ACCESSION   DANBWH010000148 DANBWH010000000
VERSION     DANBWH010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6632
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.153"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            166..567
                     /locus_tag="PPY92_002039"
     CDS             166..567
                     /locus_tag="PPY92_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDJ2626024.1"
                     /translation="MKKVNNDTVFGILQLETLLGDINSIFSEIESEYKMSREEILILL
                     TLWQKGSMTLKEMDRFVEVKPYKRTRTYNNLVELEWIYKERPVDDERTVIIHFNEKLQ
                     QEKVELLNFISDAIASRATAMQNSLNAIIAV"
     gene            complement(690..1253)
                     /locus_tag="PPY92_002040"
     CDS             complement(690..1253)
                     /locus_tag="PPY92_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDJ2626025.1"
                     /translation="MKLERILPFSKTLIKQHITPESIVVDATCGNGNDTLFLAEQVPE
                     GHVYGFDIQDLALENTRDKVKDFNHVSLIKDGHENIEHHINDAHKGHIDAAIFNLGYL
                     PKGDKSIVTKPDTTIQAINSLLSLMSIEGIIVLVIYHGHSEGQIEKHALLDYLSTLDQ
                     KHAQVLQYQFLNQRNHAPFICAIEKIS"
     gene            complement(1250..2203)
                     /locus_tag="PPY92_002041"
     CDS             complement(1250..2203)
                     /locus_tag="PPY92_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500380.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDJ2626026.1"
                     /translation="MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRD
                     GTVAHGGCTFCSAAGSGDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTH
                     APVEVLKEKFEPVLKEPGVVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIH
                     QSTSDLINRAHDMKTYYDGVAKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDV
                     QGIKIHLLHLLKGTPMVKQYDKGLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGP
                     IDIMVGPMWSVNKWEVLNGIDAELARRNSYQGLRYKSKVKQ"
     gene            2313..3494
                     /locus_tag="PPY92_002042"
     CDS             2313..3494
                     /locus_tag="PPY92_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDJ2626027.1"
                     /translation="MNIPKSVWWLVIGMALNITGSSFLWPLNTIYMKQELGKSLTVAG
                     LVLMINSFGMVIGNLLGGSLFDKLGGYKTILIGTFTCLCSTTLLNFFHGWPWYAVWLV
                     MLGFGGGMIIPAIYAMAGAVWPNGGRQTFNAIYLAQNIGVAVGAAMGGFVAEFSFNYI
                     FLANLIMYVVFALVAVTQFNIEINAKVKYPTHLDITGKKNKARFISLVLICAMFAICW
                     VAYIQWESTIASFTQSINISMAQYSVLWTINGIMILVAQPLIKPVLYLLKGNLKKQMF
                     VGIIIFMLSFFVTSFAENFTIFVVGMIILTFGEMFVWPAVPTIANQLAPDGKQGQYQG
                     FVNSAATVGKAFGPFLGGVLVDAFNMRMMFIGMMLLLVFALILLMVFKENNTQPKKID
                     A"
     gene            3782..6199
                     /locus_tag="PPY92_002043"
     CDS             3782..6199
                     /locus_tag="PPY92_002043"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDJ2626028.1"
                     /translation="MLNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPS
                     GAGLHVGHPEGYTATDIISRYKRMQGYNVLHPMGWDAFGLPAEQYALDTGNDPREFTK
                     KNIQTFKRQIKELGFSYDWDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCP
                     ALGTVLSNEEVIDGVSERGGHPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDM
                     QRNWIGRSEGAKVSFDVDNTEGKVEVFTTRPDTIYGASFLVLSPEHALVNSITTDEYK
                     EKVKAYQTEASKKSDLERTDLAKDKSGVFTGAYAINPLSGEKVQIWIADYVLSTYGTG
                     AIMAVPAHDDRDYEFAKKFDLPIIEVIEGGNVEEAAYTGEGKHINSGELDGLENEAAI
                     TKAIQLLEQKGAGEKKVNYKLRDWLFSRQRYWGEPIPVIHWEDGTMTTVPEEELPLLL
                     PETDEIKPSGTGESPLANIDSFVNVVDEKTGMKGRRETNTMPQWAGSCWYYLRYIDPK
                     NENMLADPEKLKHWLPVDLYIGGVEHAVLHLLYARFWHKVLYDLGIVPTKEPFQKLFN
                     QGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLRLYEMFMGPLDAAIAWSEKGLDGS
                     RRFLDRVWRLMVNEDGTLSSKIVTTNNKSLDKVYNQTVKKVTEDFETLGFNTAISQLM
                     VFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSKLGHEESITYQPWPTYDEALL
                     VDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVKASIEGKDIMKVIAVPQK
                     LVNIVAK"
     gene            6221..6532
                     /locus_tag="PPY92_002044"
     CDS             6221..6532
                     /locus_tag="PPY92_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDJ2626029.1"
                     /translation="MKSITTDELKNKLFESKPVQIVDVRTDEETAMGYIPNAKLIPMD
                     TIPDNLNSFNKNEIYYIVCAGGVRSAKVVEYLEANGIDAVNVEGGMHAWGDEGLEIKS
                     I"
BASE COUNT         2215 a          956 c         1283 g         2178 t
ORIGIN      
        1 acttatttaa ttttattcaa ctttgacaat tgaatagaaa gcaagtttat ttacacttgt
       61 agttttatgc ataagttagc acatacaagt tttgggattg ttgggatgtt tgttaatact
      121 tgtatggtag ctaaatatgt gattattaat tgggagatgt ttagcatgaa aaaagtaaat
      181 aacgacactg tatttgggat tttgcaatta gaaacacttt tgggtgacat taactcaatt
      241 ttcagcgaga ttgaaagcga atacaaaatg tctagagaag aaattttaat tttactaact
      301 ttatggcaaa aaggttctat gacgcttaaa gaaatggaca gatttgttga agttaaaccg
      361 tataagcgta cgagaacgta taataattta gttgaattag aatggattta caaagagcgt
      421 cctgttgacg atgaaagaac agttattatt catttcaatg aaaagttaca acaagagaaa
      481 gtagagttgt tgaatttcat cagtgatgcc attgcaagta gagcaacagc aatgcaaaat
      541 agtttaaacg caattattgc tgtgtaagtt taatagcata aaaagaggtt ttcattaagt
      601 tgaaaacctc tttttgttgt tgttggtatt aatttttcaa atgttgacta ctcaatccta
      661 aattataaat agtatagcgc agcaaatgct taagaaattt tttctatggc acaaatgaat
      721 ggagcatgat tacgttggtt taaaaattga tattgcaaaa cttgcgcatg cttttgatcc
      781 aaagtactca agtaatcaag caatgcatgc ttctcaattt gtccttcgct atgaccatga
      841 tatataacaa gtacaataat accttcaatt gacattaatg atagcaatga attaatagct
      901 tggattgtcg tgtcaggctt tgtcacgata gatttatcac ctttaggcaa ataacctagg
      961 ttaaagatgg ctgcatcaat atgaccttta tgtgcatcat ttatatgatg ttcaatattt
     1021 tcatgtccat cttttattaa agaaacatga ttgaaatcct taactttatc acgtgtattt
     1081 tccaaagcta aatcttgaat gtcgaaacca taaacatgtc cttctggtac ttgttcggct
     1141 aaaaataaag tgtcattgcc gttaccgcaa gttgcgtcca caacaatact ttctggtgtt
     1201 atatgttgtt taataagtgt ttttgaaaaa gggagtatac gttctaattt cattgcttca
     1261 ccttagactt gtaacgcaag ccttgataag aatttctacg tgctaattca gcatcgatgc
     1321 catttaatac ttcccattta ttaacactcc acattggacc taccatgata tctattggac
     1381 catcaccggt aattcggtga acgatcattt cagggggaat cacttctaat tggtccacaa
     1441 ctaggtttgt gtactcttct tgagtcataa aagttaataa acctttatcg tattgtttta
     1501 ccatcggtgt accttttaac aaatgaagta aatgaatttt aataccttgt acatccattt
     1561 gtgcaacttc tttggcagta gccatcatca tgtcatagtc ttcgccaggt aagccattaa
     1621 tgatgtgtgt acatacattg atattatgct tacgtaattt tgccacacca tcataataag
     1681 ttttcatatc atgggcacga ttgattaaat cagatgttga ctgatggatt gtttgtagtc
     1741 ctaattcaac ccataagtat gttcgttgat tcaaatctgc taaatattcg acaacatcgt
     1801 ctggtagaca gtcaggacgc gtaccaatag ataatcctac aacacccggt tctttaagta
     1861 caggttcgaa tttttctttt aatacttcaa ctggtgcatg tgtatttgta aatgcctgaa
     1921 aataagcaat atattttcct tcgtgccatt tctcatgcat cttttcctta atttctttaa
     1981 attgtactgc gattgaatct gcacgattac ctgcaaagtc cccgctacct gcagcagaac
     2041 aaaatgtaca tccaccatgt gctacagtgc catcgcggtt aggacagtca aacccgccat
     2101 ccaatgcaac tttaaatatt ttttgtccaa atttattttt taaatggtaa ttccatgtgt
     2161 gataacgttt gttttcaaaa gcgtattgga aatgattgcc catatgtcat tttcctttct
     2221 ataaaaaaag agttctaagt acagatttta acatatttta atgttatagt gtttattata
     2281 gtttgacaaa aaagagagag gaactatgaa atatgaatat acctaaatca gtctggtggc
     2341 tagtaattgg catggcgtta aatattactg gttccagttt tttgtggcct ttaaatacaa
     2401 tttatatgaa acaagaactt ggaaaaagtt taactgttgc tggtttagtg ctaatgataa
     2461 attcatttgg catggttatt ggaaacttat taggtggttc actatttgat aaattaggtg
     2521 gatacaagac gattttaatt ggaactttca cttgtctttg tagtacaacg ctacttaatt
     2581 tctttcacgg gtggccttgg tatgctgtat ggcttgtaat gttaggcttt ggtggcggaa
     2641 tgattattcc tgcgatatac gctatggctg gagcagtgtg gccgaatggc ggaagacaaa
     2701 cgtttaatgc gatatactta gcgcaaaata ttggtgtggc tgtcggtgct gcaatgggcg
     2761 gctttgtcgc agaatttagc tttaactata tctttttagc caatcttatt atgtatgttg
     2821 tgtttgcgct tgtcgcggta acgcaattta atattgaaat taatgcgaaa gttaaatatc
     2881 caactcattt agatattact ggtaaaaaga ataaagcaag atttatttca ttagtactaa
     2941 tttgtgcaat gtttgcaatt tgttgggttg cgtatattca atgggagtct acaatcgcat
     3001 catttacaca atctattaat atttcaatgg cacaatatag tgttttatgg acaattaacg
     3061 gaataatgat tttagtagca caaccattaa ttaaaccggt tctctatctg ttaaaaggaa
     3121 acttaaagaa gcaaatgttt gtcggcatca tcatttttat gttgtcgttc tttgtcacga
     3181 gttttgccga aaactttaca atatttgttg tcggtatgat tattttaact tttggagaaa
     3241 tgtttgtatg gccagcagtt ccaactatag ccaatcagtt agcgccagat ggtaagcaag
     3301 gacagtacca aggttttgtg aattcagctg ctacagtagg aaaagcattt ggtccatttc
     3361 ttggtggtgt attagttgat gcgtttaata tgcgcatgat gtttatcggt atgatgctac
     3421 tacttgtatt tgcattaata ttattaatgg ttttcaagga gaataatacg caacctaaaa
     3481 aaatagatgc ataatgagta aatagaatta acgttataga cttgaaataa atgtcgttat
     3541 aacataatat taatttgtat aatttaattt cgtttggagc ttttctacag aaagctagtg
     3601 atgctgagag ctagtgttaa ggactaaatg taaatcgtat taattttaaa ttgaatgaat
     3661 gacatctctt actattaaaa tgagtgcaca atttttgtga aatagggtgg taacgcggca
     3721 aatgtcgtcc ctatgtaaat agaatagtta gaggtgtctt ttttattgaa taggaggaaa
     3781 tgtgttgaat tacaaccaca atcaaattga aaagaaatgg caagactatt gggacgaaaa
     3841 taaaacattt aaaacaaatg ataacttagg tcaaaagaaa ttttatgctt tagacatgtt
     3901 tccatatcca tcaggtgctg gtttacatgt tggacatcct gagggctata cagcaacaga
     3961 tatcatttca agatataaaa ggatgcaagg ttataatgta ttacatccga tggggtggga
     4021 tgcattcgga ttaccagcag agcaatatgc tttagacact ggcaacgacc cacgtgaatt
     4081 tacaaagaaa aatatccaaa cttttaaacg acaaattaaa gaattagggt tcagttatga
     4141 ttgggatcgt gaagttaata caacagatcc agaatactat aaatggacac agtggatttt
     4201 catacagtta tataacaaag gtttagcata cgttgatgaa gttgcagtta actggtgtcc
     4261 agcattaggc actgttttat ctaacgaaga agtgattgat ggtgtctctg aacgtggtgg
     4321 acatccagtt tatcgtaagc cgatgaaaca atgggtactt aaaatcacag aatatgcaga
     4381 tcaattatta gcagatttag atgatttaga ttggcctgag tctttaaaag atatgcagcg
     4441 caattggatt ggacgttctg aaggggccaa agtttcattt gatgtagata atacggaagg
     4501 aaaagtagaa gtatttacga ctagaccaga cactatctat ggtgcatcat tcttagtctt
     4561 aagtcctgaa catgcattag ttaattcaat tacaactgat gaatataaag aaaaagtaaa
     4621 agcttatcaa acagaagctt ctaaaaagtc agatttagaa cgtacagatt tagcaaaaga
     4681 taaatcaggt gtgtttactg gtgcatatgc aattaatcct ttatctggtg aaaaagtaca
     4741 aatttggatt gctgattatg tattatcaac atatggtact ggagcaatta tggcagtacc
     4801 agcgcatgat gacagagatt atgaatttgc taaaaagttt gatttgccaa tcattgaagt
     4861 catcgaaggt ggaaatgttg aagaagcagc atacactggt gaaggtaaac atattaattc
     4921 tggtgaactt gatggtttag aaaatgaagc ggcaattact aaagctattc aattattaga
     4981 gcaaaaaggt gctggcgaaa agaaagttaa ttacaaatta agagattggt tattcagtcg
     5041 tcagcgttat tggggcgaac caattcctgt cattcattgg gaagatggaa caatgacaac
     5101 tgttcctgaa gaagagctac cattgttgtt acctgaaaca gatgaaatca agccatcagg
     5161 gactggtgag tctccactag ctaatattga ttcatttgta aatgttgtag atgaaaaaac
     5221 aggtatgaaa ggacgtcgtg aaacaaatac aatgccacaa tgggcaggta gttgttggta
     5281 ttatttacgt tacatcgatc ctaaaaatga aaatatgtta gcagatcctg aaaaattaaa
     5341 acattggtta cctgttgatt tatatatcgg tggagtagaa catgcggttc ttcacttatt
     5401 atatgcaaga ttttggcata aagtccttta tgatttgggt atcgtaccta ctaaagaacc
     5461 tttccaaaaa ttatttaacc aaggtatgat tttaggagaa ggtaatgaga agatgagtaa
     5521 atctaaagga aatgtaatca atcctgatga tatagtacag tctcatggtg cagatacttt
     5581 acgtctttac gaaatgttta tgggaccttt agatgctgca attgcatgga gtgaaaaagg
     5641 attagatggg tctcgtcgat tcttagatcg cgtatggcgt ttaatggtaa atgaagatgg
     5701 aacattgagt tcaaaaattg taactacaaa taataaatct ttagataaag tttataacca
     5761 aactgttaaa aaggtaacag aagactttga aacattagga tttaatactg ctattagtca
     5821 attaatggta tttattaatg agtgttataa agttgatgaa gtttataaac cttacattga
     5881 aggcttcgtt aaaatgttag cacctattgc accacatatc ggtgaagaat tatggtcaaa
     5941 attaggacat gaagagtcta ttacgtacca accttggcca acttatgacg aagcactact
     6001 tgtagatgat gaagtagaaa tcgttgttca agtaaatggt aaattgagag ctaaaattaa
     6061 aattgctaaa gatacatcaa aagaagaaat gcaagaaatt gccttatcta atgacaatgt
     6121 taaagcgagt attgaaggta aagacatcat gaaagtcatc gctgttcctc aaaaattagt
     6181 caatattgta gctaaataat gttttaagga ggactttgaa atgaagtcaa ttactacaga
     6241 tgaattaaaa aacaaacttt ttgaatctaa accagttcaa attgttgatg ttcgtactga
     6301 cgaagaaaca gcaatgggat atattcctaa tgcaaagttg attccaatgg ataccattcc
     6361 ggataattta aattcattta ataaaaatga aatatattat attgtatgtg ctggtggagt
     6421 tcgaagcgct aaagttgtag aatatttaga ggcaaatggc attgatgccg taaatgtcga
     6481 aggcggcatg cacgcatggg gcgatgaagg tttggaaata aaaagtattt aaagtagtga
     6541 cataatttaa aataatatta catttgtaat gacaccaagt aacgtttcgg ttgcttggtg
     6601 ttttttggta tgaattactt tctgttacaa aa
//
LOCUS       DANBWH010000149         6602 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.154, whole genome shotgun sequence.
ACCESSION   DANBWH010000149 DANBWH010000000
VERSION     DANBWH010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6602
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.154"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..1445
                     /locus_tag="PPY92_002045"
     CDS             <1..1445
                     /locus_tag="PPY92_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDJ2626030.1"
                     /translation="EKAIINRLNLFKEMGRPAQCVFLSWNRYLYRNAQNYITSSDYIN
                     MYDFFQEATYLERNEPFDWLSYWTDECHYTLKHVENSHDFRIYDQERFLMYAHFQDPK
                     YRILDYVNHFDSQRRKVKRDFYDVRGFLSCSRILVDKQQTLCEFFYNPEGDTKLEKYF
                     SYKDGKPEVQKIIVYYANKQYFFNNETELGAFFIKQLYQHGDLFFSDRNVYTAPIFNL
                     TPESIPVVAVLHSTHIKNIDALDSSPFKNVYKAMFENLSRYRAIIVSTEQQKLDVEKR
                     INHTIPIVNIPVGYSETIDTPVQTLDQRSVKLISVARYSPEKQLHQQIELIKRLVSYV
                     PKIELHMYGFGSESKKLNELIQKYGLENHVYLRGFLSNLNQEYSDAYLSLITSNMEGF
                     SLALLESLAHGVPVISYDIKYGPNELITPDFNGYLITKNDEDALFDKVKYVIDHPEVQ
                     QRLSKGSLAKAQQYSKASLIKQWDQFVRLI"
     gene            complement(1570..3042)
                     /locus_tag="PPY92_002046"
     CDS             complement(1570..3042)
                     /locus_tag="PPY92_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDJ2626031.1"
                     /translation="MNYILGTILESKITGVEKAQINRLKLFKQHGISSKCVYVKWNPY
                     SYTYAKQHQIENDVFTMYDYFQKAINYKKTKQVNWIQYWEKSCRYTLKFVENSNDVRI
                     YDEEQFVMYAHFLDKQYHQLNYVNYFDHKRRKVKRELYDGRGFLSCSRILGEGQRIVL
                     ENYYTPNGEIVIQKYFDDIKGKNTLTKVILNEDQQQQFFDTEDELVQYFLHQLCKNND
                     QIILDRPHELGNVIAGLNQSIPVVVVLHSTHLSGTGNGIKSFYKTVFNNLTRYKAIVV
                     STEQQCQDISQYIENKIPVINIPVGYVANLKYQFDINQKEKNHIISIARLVENKQIKH
                     QIEVIKQLVTKHPNIQLNIYGHGNGLSEYRQLVEDYHLSEHVKFHGFKTHINEEIAKA
                     ELMLSTSKMEGFGLAILESLSVGTPVISYDIDYGPSELIQDGFNGYLVPKGDINQMVE
                     KVDQLLNNTQKLQQFSINSIESAQQYNATTISTKWQNILN"
     gene            complement(3163..6588)
                     /gene="sdrE"
                     /locus_tag="PPY92_002047"
     CDS             complement(3163..6588)
                     /gene="sdrE"
                     /locus_tag="PPY92_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrE"
                     /protein_id="HDJ2626032.1"
                     /translation="MINRDNKKAITKKGMISNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAENTSTENAKQDDATTSDNKEVVSETENNSTTENDSTNPIKKETNTDSQP
                     EAKEESTTSSTQQQQNNVTATTETKPQNIEKENVKPSTDKTATEDTSVILEEKKAPNY
                     TNNDVTTKPSTSEIQTKPTTPQESTNIENSQPQPTPSKVDNQVTDATNPKEPVNVSKE
                     ELKNNPEKLKELVRNDNNTDRSTKPVATAPTSVAPKRLNAKMRFAVAQPAAVASNNVN
                     DLITVTKQTIKVGDGKDNVAAAHDGKDIEYDTEFTIDNKVKKGDTMTINYDKNVIPSD
                     LTDKNDPIDITDPSGEVIAKGTFDKATKQITYTFTDYVDKYEDIKARLTLYSYIDKQA
                     VPNETSLNLTFATAGKETSQNVSVDYQDPMVHGDSNIQSIFTKLDENKQTIEQQIYVN
                     PLKKTATNTKVDIAGSQVDDYGNIKLGNGSTIIDQNTEIKVYKVNPNQQLPQSNRIYD
                     FSQYEDVTSQFDNKKSFSNNVATLDFGDINSAYIIKVVSKYTPTSDGELDIAQGTSMR
                     TTDKYGYYNYAGYSNFIVTSNDTGGGDGTVKPEEKLYKIGDYVWEDVDKDGVQGTDSK
                     EKPMANVLVTLTYPDGTTKSVRTDANGHYEFGGLKDGETYTVKFETPAGYLPTKVNGT
                     TDGEKDSNGSSITVKINGKDDMSLDTGFYKEPKYNLGDYVWEDTNKDGIQDANEPGIK
                     DVKVTLKDSTGKVIGTTTTDASGKYKFTDLDNGNYTVEFETPAGYTPTVKNTTAEDKD
                     SNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKDVKVTL
                     LNEKGEVIGTTKTDENGKYRFDNLDSGKYKVIFEKPAGLTQTVTNTTEDDKDADGGEV
                     DVTITDHDDFTLDNGYFEEDTSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDAGKHTPV
                     KPMSTTKDHHNKAKALPETGSENNGSNNATLFGGLFAALGSLLLFGRRKKQNK"
BASE COUNT         1905 a         1118 c         1025 g         2554 t
ORIGIN      
        1 tagaaaaagc aattattaat cgtttaaatt tatttaaaga aatgggaaga ccagcgcaat
       61 gtgtatttct gtcatggaat agatatttat atagaaatgc ccaaaattat attactagtt
      121 ctgactatat taatatgtat gatttcttcc aagaagcaac atatttagaa cgcaatgaac
      181 catttgactg gttgtcgtat tggactgatg aatgtcatta tactttaaaa catgttgaaa
      241 atagtcatga ctttagaata tatgatcaag agcgtttctt aatgtatgcc cattttcaag
      301 atcctaaata tcgtatatta gattatgtga atcattttga tagtcaaaga cgaaaagtaa
      361 aacgtgattt ttatgatgtg cgtggctttt taagttgtag taggattctt gttgataaac
      421 aacaaacatt atgtgaattt ttctataatc cagaaggtga cacaaaatta gaaaagtatt
      481 tttcttacaa ggatggcaaa ccagaagtac aaaaaattat tgtttactat gctaacaaac
      541 aatatttctt taataatgaa acggaattag gcgcattttt cattaaacaa ctatatcaac
      601 atggggattt atttttcagt gaccgtaatg tgtatacagc acctattttc aacttaacac
      661 ctgagtcaat cccagtagta gctgtgcttc acagtacaca cattaaaaat attgatgcat
      721 tagattcatc gccatttaaa aatgtatata aagcgatgtt tgaaaattta tctcgatatc
      781 gtgcaattat cgtttctact gagcagcaaa aattagatgt tgaaaaacgt attaaccata
      841 ctattccaat tgttaatatt cctgtcggct atagtgaaac aattgataca ccagtccaaa
      901 cattagatca gcgcagtgtt aaattaattt ccgttgctcg ttattcacct gaaaaacaat
      961 tacatcaaca aattgaatta attaaacgct tagtatcgta tgtcccaaaa atagagcttc
     1021 atatgtatgg ttttggttct gaaagtaaaa aattaaatga attgatacaa aaatatggat
     1081 tggaaaatca tgtttactta cgtggctttt taagcaatct aaaccaagaa tacagcgatg
     1141 cttacttaag tttaataaca agtaacatgg aaggtttctc tttagcgcta cttgaatctt
     1201 tagcacatgg tgttccagtt attagttatg atattaaata tggacctaac gaattgatta
     1261 cgcctgattt caatggttat ttaattacta aaaatgatga agatgcccta tttgataaag
     1321 taaaatatgt gattgatcat cctgaagtac aacaacgact gtcaaaaggt agtttggcaa
     1381 aagcgcaaca atatagtaaa gcgtcgttaa ttaaacaatg ggaccaattt gttcgtctta
     1441 tataaatgta cttcaccttt atttttataa aaaaaagcgt tttcctatgt catttaacta
     1501 aacatgtaag ttcgtatgaa cgttgtttgt taaatagatg attctaggaa aatgcttttt
     1561 tcttttgact tagtttaaaa tattttgcca ctttgtactg atagtagttg cattgtactg
     1621 ttgtgcagat tctatgctat taatcgaaaa ctgttgcaat ttttgagtat tatttagtaa
     1681 ttgatcgacc ttttcaacca tttgattgat gtcaccttta ggtactaaat agccattaaa
     1741 tccatcttga atcagttctg atggcccata atctatatca taactgatca ctggtgtacc
     1801 tactgaaagc gactctaaaa ttgctaagcc aaaaccttcc attttacttg tcgataacat
     1861 cagttctgct ttagcaatct cttcattaat atgcgtctta aaaccatgaa atttaacatg
     1921 ttccgataaa tgataatctt ctacaagttg tcgatattct gacaaaccat ttccatgtcc
     1981 ataaatattc aattgaatat tgggatgttt tgttactaat tgcttgatca cttcaatttg
     2041 atgtttaatt tgtttatttt caacgaggcg agcaattgat atgatatgat ttttctcctt
     2101 ttgattgatg tcaaattgat actttaaatt tgccacgtag ccaaccggaa tattgataac
     2161 tggtatttta ttttcaatat attgtgaaat atcttggcat tgctgttctg ttgatacaac
     2221 aatcgcttta taacgtgtta aattattaaa tactgtttta taaaaacttt ttataccatt
     2281 accggtaccg gataaatgtg tactatggag cacaacaaca actggaatac tttgatttaa
     2341 tcccgctata acatttccta attcatgagg acgatctaat atgatttgat cattattttt
     2401 acataattga tggagaaaat attgaactaa ttcatcttct gtatcaaaaa attgttgctg
     2461 ctggtcttca tttaagataa cctttgtgag cgtgtttttt ccttttatat cgtcgaaata
     2521 tttttggatg acgatttccc cattaggtgt atagtaattt tcgagtacaa tccgttgtcc
     2581 ttcacctaaa attcgagaac aacttaaaaa gcctcttcca tcatacaatt cgcgttttac
     2641 ttttcttctt ttatgatcaa aataattcac atagtttaat tgatgatact gtttatctaa
     2701 aaaatgagca tacattacaa attgctcttc atcatatatt ctgacatcat ttgaattttc
     2761 cacaaatttc aatgtgtacc tacatgactt ttcccaatac tgtatccagt taacttgctt
     2821 tgtcttttta taattgattg ctttttgaaa atagtcatac attgtaaata catcattttc
     2881 aatctgatgt tgcttcgcat atgtgtatga ataaggattc catttaacat atacacattt
     2941 tgaagatatg ccgtgttgtt tgaacaactt caatctattt atttgcgctt tttctacacc
     3001 tgtaatttta ctttctaaaa ttgttcctaa aatgtaattc atattatcgc ctcatataag
     3061 ttttattccg tatctttatt gtttatttta tatgaaaaat acatctattg catgtgtaat
     3121 cataaaaaaa aaccaggcca caaggacctg ggtcatattg tattatttgt tttgtttttt
     3181 gcgacgaccg aataacaata atgaacctaa tgctgcaaat aatccaccaa ataacgttgc
     3241 gttatttgag ccgttatttt cactacctgt ttctggtaat gcttttgctt tattgtgatg
     3301 gtctttagta gtactcattg gtttaacagg tgtatgtttt cctgcatccg agtccgaatc
     3361 gctatctgag tctgaatcac tgtctgagtc tgagtcgcta tctgagtctg agtcgctgtc
     3421 tgaatctgag tcgctatctg agtctgaatc actgtccgaa tctgagtcgc tatctgaatc
     3481 tgaatcgctg tctgagtctg agtcactgtc tgagtctgag tcgctatctg agtctgagtc
     3541 actgtctgaa tctgagtcgc tatctgagtc tgaatcgctg tctgaatctg agtcgctatc
     3601 tgagtctgaa tcgctgtccg aatctgagtc gctatctgag tctgagtcgc tgtctgagtc
     3661 tgagtcgcta tctgagtctg agtcgctgtc tgaatctgaa tcgctatctg agtctgaatc
     3721 actgtctgaa tctgagtcgc tgtctgagtc tgaatcactg tctgagtctg aatcactgtc
     3781 tgatgtatct tcttcgaagt atccgttatc aagtgtgaaa tcatcatgat ccgtaattgt
     3841 tacgtcaact tcgccaccat cggcatcttt atcatcttca gttgtatttg taactgtttg
     3901 tgttaagcca gcaggctttt caaaaataac tttgtattta ccgctatcta aattatcgaa
     3961 acgatattta ccattttcat ctgttttagt tgttccaatt acttcgcctt tttcatttaa
     4021 taaagtaact ttaacatctt tgataccttt ttcagttgaa tcttgtttac cgtctttatt
     4081 actgtcgtac caaacataat cacctaaact gtattttggt gttttataga aaccactgtc
     4141 taatgtcata ttatctgcat ctttaatgac acctgttgtt gttaaaccat tagaatcttt
     4201 atcttcagct gtagtatttt taaccgttgg cgtgtaacct gctggtgttt caaattctac
     4261 tgtatagtta ccattatcta aatctgtaaa tttatattta cccgaggcat cagtagtagt
     4321 tgtaccaata acttttccag tactatcttt taatgtaacc ttaacatctt tgataccagg
     4381 ttcattagca tcttggatac catctttatt tgtatcttcc catacatagt caccaagatt
     4441 atatttaggt tctttataaa aaccagtgtc taaagacata tcatctttac cattaatttt
     4501 aacagttata gaactaccat ttgagtcttt ttcaccatca gttgttccat ttacttttgt
     4561 tggaagatat ccagctggcg tttcgaattt aactgtataa gtttctccgt ctttcaaacc
     4621 accgaattca taatgaccgt tagcatctgt tcttactgat tttgtagtac cgtccgggta
     4681 agttaatgta actaaaacgt ttgccattgg cttttctttc gaatctgtac cttggacacc
     4741 gtctttatca acgtcttccc atacatagtc accaattttg tataactttt cttcaggttt
     4801 aacagtaccg tcgccaccgc cagtgtcatt agaagttacg atgaagtttg aatatcctgc
     4861 ataattataa taaccatatt tatcagttgt tctcatacta gtaccttgag caatatctag
     4921 ttcgccatct gatgtaggtg tatatttact aacaactttg ataatatagg ctgaattaat
     4981 atcaccaaaa tccaatgttg ctacattatt actaaatgat tttttattat caaattgact
     5041 tgttacatct tcgtattgac taaaatcata gattctatta ctttgaggca attgttgatt
     5101 agggttaact ttataaactt ttatttctgt attttggtca ataatggtac taccatttcc
     5161 tagtttaata tttccataat catctacttg actaccagct atatcaactt tagtgttagt
     5221 tgctgttttt ttcaaaggat taacataaat ttgttgttca atagtttgtt tgttttcatc
     5281 taactttgta aagatagatt gaatgtttga atcaccatga accattgggt cttgataatc
     5341 aacagaaacg ttttggctag tttctttacc tgctgttgca aacgttaaat tcaaactagt
     5401 ttcattaggt actgcttgct tatcaatata tgagtataaa gttaaacgtg cttttatatc
     5461 ttcatattta tctacataat ctgtaaatgt atatgtgatt tgcttagtcg ctttatcaaa
     5521 tgttcctttg gcaatgacct ctcctgatgg atcagtaata tcgataggat catttttatc
     5581 tgttaaatcc gaaggaatta cattcttatc ataattaatc gtcattgtat cgcctttttt
     5641 gactttattg tcaattgtaa actctgtatc atattcaata tctttaccgt catgcgctgc
     5701 tgccacatta tctttaccat cgccaacttt gatcgtctgt ttcgtaactg taattaagtc
     5761 atttacatta tttgaagcaa ctgctgctgg ttgtgcaact gcaaaacgca ttttcgcatt
     5821 taatcgtttt ggtgcaacac ttgttggagc tgtagcaact ggtttagttg aacgatctgt
     5881 attgttatca tttctaacta attcttttaa tttctcagga ttatttttaa gttcttcttt
     5941 tgacacattt actggttctt ttggattagt tgcatctgta acttgattgt ctacttttga
     6001 aggcgttggt tgcggttgtg aattttcaat atttgtagat tcttgaggtg tagttggttt
     6061 tgtttgaatt tcacttgtag atggttttgt agttacatcg ttatttgtat aatttggtgc
     6121 tttcttctct tctaaaataa cagatgtatc ttctgtcgca gttttatcag ttgaaggttt
     6181 aacattttct ttttcaatgt tttgaggctt agtttcagtt gtagctgtaa cgttattttg
     6241 ctgttgttga gtacttgatg tagttgattc ttctttagct tctggttgtg aatcagtatt
     6301 tgtttctttc ttaattggat ttgttgaatc attttctgtt gtcgaattat tttcagtttc
     6361 cgacactact tctttattat cactagtcgt tgcatcatct tgtttcgcat tttctgtact
     6421 agtgttttca gcagctttag cttcttggtt ccctagacca aaaatcaatg tcgtacctac
     6481 taaaatcgat gcagttccta cagtatactt tctaatcgaa aatttgttta agcgatttga
     6541 aatcataccc ttttttgtta ttgccttttt attatccctg ttaatcatat aaatctcctt
     6601 ta
//
LOCUS       DANBWH010000150         6592 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.155, whole genome shotgun sequence.
ACCESSION   DANBWH010000150 DANBWH010000000
VERSION     DANBWH010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6592
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.155"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..1323)
                     /locus_tag="PPY92_002048"
     CDS             complement(<1..1323)
                     /locus_tag="PPY92_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDJ2626033.1"
                     /translation="MENNELQRGLSARQIQMIALGGTIGVGLFMGATSTIKWTGPSVI
                     LAYLIAGIFLFLIMRAMGEMIYLNPTTGSFATFASDYIHPAAGYMTAWSNIFQWIVVG
                     MSEVIAVGEYMKFWFPELPTWIPGVIAILLLMAANLFSVKAFGEFEFWFALIKVVTII
                     LMIIAGFGLIFFGFGNGGHAVGISNLWTNGGFMPNGIVGFFFALSIVIGSYQGVELIG
                     ITAGETKDPQKNIVKAVNGVIWRILIFYLGAIFVIVSVYPWNQLGDIGSPFVATFAKI
                     GITFAAGLINFVVLTAAMSGCNSGIFSASRMIYTLAHKGEMPKIFTKIMRNGVPLYTV
                     VAVSLGILIGALLNVILPLYIDGAKSIFVYVYSASILPGMIPWFMILFSHLRFRKLHP
                     EELEGHPFKMPGGAVTNYLTILFLILVLVGMVFNVETRISVLIGVIFLT"
     gene            1603..1959
                     /locus_tag="PPY92_002049"
     CDS             1603..1959
                     /locus_tag="PPY92_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626034.1"
                     /translation="MLKQILPRATKISILFAVAFFIINYIGMEKPDILYLVGRTIIAT
                     LAFILICLTLFTIINSPERKIKLGTTLPIALIIGIIFGAIFLTVQIGVITGLIIGVIA
                     TFIWELIEKNKGGRSS"
     gene            1956..2141
                     /locus_tag="PPY92_002050"
     CDS             1956..2141
                     /locus_tag="PPY92_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626035.1"
                     /translation="MTTALIGVIVLILLIISFVPNYRAMKSAKEQGSNATRATIMVGI
                     DIILIVLIIVTLLLKYM"
     gene            complement(2237..2428)
                     /locus_tag="PPY92_002051"
     CDS             complement(2237..2428)
                     /locus_tag="PPY92_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626036.1"
                     /translation="MMGYIILFFLAGPVILGVGNLVIGPIFNKQTPFRVQVRSFVVGS
                     MIYLILATIGYFLLLQGKL"
     gene            complement(2439..3074)
                     /locus_tag="PPY92_002052"
     CDS             complement(2439..3074)
                     /locus_tag="PPY92_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDJ2626037.1"
                     /translation="MYRAVIFDFDGTIIDTEQHLFNVINKHLEMHNADPISIDFYRSS
                     IGGAATDLHDHLIKAIGSENKDKLYEEHHLTSTTLPMIDTIKSLMAFLKQRHIPMAIA
                     TSSVKAEIMPTFKALGLDDYIEVVVGREDVEQVKPDPELYLSAVQQLNYMPTQCLAIE
                     DSVNGATAAIAAGLDVIVNTNKMTSAQDFSNVDYVAKDIDYDQIVARFFTK"
     gene            complement(3149..4066)
                     /locus_tag="PPY92_002053"
     CDS             complement(3149..4066)
                     /locus_tag="PPY92_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HDJ2626038.1"
                     /translation="MFQKLSIFATKSFLVWMLVAAVIGFIFPQHVATLGKWVPYLLGI
                     VMLGMGLTITPNDFKMVFKAPRAVIIGVCLQFSIMPTLAFIIAKSFHLPPDIAVGVIL
                     VGCCPGGTSSNVMSYLAKANVALSVSITTVSTLLAPFVTPALIYLFANEWLEVSFLSM
                     LWSVVQVVLIPIALGIVLQIINRKIAEKASTALPIISVVAISLILAIVVGGSKHQILT
                     TGLLIFLVVILHNVLGYTIGYWLARLLKLDRQDQKAVSIEVGMQNSGLAVSLAALHFN
                     PIAAVPGAVFSFIHNITGPILAKYWSKKL"
     gene            4334..4864
                     /locus_tag="PPY92_002054"
     CDS             4334..4864
                     /locus_tag="PPY92_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626039.1"
                     /translation="MLIDKFETYIINIAGLNDRTTRKKLSKLCKSVQFCDALQFSINK
                     QFNQYVLEISLPKQQLPYFISFLSFHQYSIFQVLSPKKINELLDSDNLYQSAKRFDIN
                     IDGLQDAFIKDKVIDIMNMFQNHTDITYTLNKSHAHIICTPEIFAKLLHTIATRNIDI
                     LSANYRSSSMSKARIS"
     gene            complement(4930..6534)
                     /locus_tag="PPY92_002055"
     CDS             complement(4930..6534)
                     /locus_tag="PPY92_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDJ2626040.1"
                     /translation="MNAIKRFGSAMIVPVLMFAFFGIVLGFATLFKNPTIMGSLADQH
                     TFWFKFWSVIESGGWVIFTHMEVVFVVGLPLSLAKKAPGHAALAALMGYLMFNTFINA
                     ILTQWPHTFGANLEKGVENVPGLKSIAGIATLDTNILGGIIISAIITWIHNRYYSKRL
                     PEMVGVFQGLTFVVTISFFVMLPLAAITCVIWPTVQHGIGSMQHFIIASGYIGVWLYH
                     FLERVLIPTGLHHFIYAPIEVGPVVVNHGLKAEWLQHLNEFAKSTKPLKEQFPYGFML
                     QGNGKVFGCLGIALAMYATTPKENRKKVAALLIPATLTAVVVGITEPLEFTFLFIAPY
                     LFVLHAVLAASMDTLMYAFGVVGNMGGGLLDFISTNWLPLGKEHWGTYVAQVIIGLIF
                     VAIYFFLFRFLILKFDIPLPGRKKTEEEVKLFSKQDYKNKKGDSVDSKRASTGNEYED
                     KAAYYLDGLGGKENIKDVTNCTTRLRLTVYDESKVADTEYFTHQQMAHGLVKSGKSIQ
                     VVVGMTVPQVREAFEQMVEDQSSEDK"
BASE COUNT         2301 a         1278 c          932 g         2081 t
ORIGIN      
        1 cgttaagaag atgacaccaa ttagcactga tattctagtt tcaacattaa ataccatacc
       61 aaccaatact aatattaaga ataagattgt taaatagtta gttactgcgc caccaggcat
      121 tttgaatggg tgaccttcca attcttcagg atgtaatttt ctaaaacgta agtgactgaa
      181 taaaatcata aaccaaggaa tcatacctgg taaaatagac gcactataaa cgtatacgaa
      241 aatactcttc gctccatcga tatataaagg taaaattacg tttaataaag caccaattaa
      301 aataccaaga gatactgcta caacagtgta caatggtaca ccatttctca tgattttagt
      361 aaatattttt ggcatttcac ctttatgagc aagtgtataa atcatacgac tcgcactgaa
      421 aatacctgag ttacatcctg acattgctgc agttaataca acaaagttaa ttaatccagc
      481 tgcaaatgta ataccgattt tagcaaatgt tgcaacaaac ggacttccaa tgtctcctaa
      541 ttgattccaa ggatacactg aaacaataac aaaaatagcg cctaaataga aaattaaaat
      601 tctccagata acaccattca ctgctttaac gatatttttc tgaggatctt tagtttcacc
      661 tgccgtaata cctatcagtt ccacaccttg gtatgaacca attacaattg ataatgcaaa
      721 gaagaaacca acaataccat ttggcataaa tccgccattt gtccatagat tagaaatacc
      781 taccgcatgg ccaccatttc caaaaccaaa gaaaataaga ccaaaaccag caataatcat
      841 taaaataatt gttacaactt taattaaagc aaaccaaaat tcaaattctc caaacgcttt
      901 taccgagaat aaattcgctg ccattaataa taaaatagca ataacaccag gaatccaagt
      961 tggcaattcc gggaaccaaa acttcatata ttctcctact gcgatgacct cactcatacc
     1021 aactacaatc cattggaata tattactcca tgctgtcata taacctgctg caggatgtat
     1081 ataatcactt gcaaatgttg caaatgatcc tgttgtaggg tttaaataaa tcatttcccc
     1141 cattgctctc atgattaaaa ataaaaagat acccgcaatt aaatatgcaa ggataactga
     1201 tgggcctgtc catttaattg tacttgtggc acccatgaaa agccccacgc caatcgtacc
     1261 accaagtgca atcatttgaa tttgacgggc actcaatccc ctttgtagtt cattgttctc
     1321 cataacttct taccccatct ctgattgata ctaaataaat ccataaaaaa acttattgcc
     1381 gtaattatac tataaattat cgattttggt acattaatat ttgcatacca tgcaatcgtt
     1441 tacagaacta ctatagcagg cttttgcaac tttgcaaaca attattttgt gatttttcta
     1501 aaaaattaaa aaagttgctc aagtaccgtt gagtcacata caacttcagc tattctcggt
     1561 aattatgata taattttaat aatattaaaa aggagtatat caatgttaaa acaaatactg
     1621 ccacgtgcta caaaaatatc gatactcttt gctgtagcat ttttcatcat aaattatatc
     1681 ggtatggaaa aacctgacat cctatattta gttggaagaa ctattattgc aacacttgct
     1741 ttcatactca tttgtcttac attatttact attattaatt ctccagagcg caagattaaa
     1801 ctcggtacga ctttaccgat tgcattaatt attggtatca ttttcggcgc catattttta
     1861 acggtacaaa tcggtgtcat cactgggtta atcatcggtg tgattgctac gtttatttgg
     1921 gaattgattg aaaaaaataa aggaggtcgt tcatcatgac aacagcatta attggtgtaa
     1981 ttgtattaat cttactaatt attagctttg tgccaaacta ccgtgctatg aaatcagcaa
     2041 aagaacaagg aagtaatgca acaagagcta caattatggt aggcatagat attatcttaa
     2101 ttgtacttat catcgtgaca ttattattaa aatatatgta actatttaaa ccaccctatc
     2161 tcatacataa acatgaacgt tctgccattt cacatacata tggtcgtcca tttaatgagg
     2221 taaggtggtt ttctcgttaa agtttacctt gtaatagtaa aaaatagcca attgttgcga
     2281 gtattaagta aatcattgaa ccaacaacaa aagatcttac ttgcacgcga aatggtgtct
     2341 gtttgttaaa tataggacca atcaccaaat ttccaacgcc taaaattact ggaccagcta
     2401 gaaaaaacaa tataatgtaa cccatcatga tacgcctcct atttcgtaaa gaaacgcgct
     2461 acaatttgat cgtaatcaat atcttttgct acataatcta cattagaaaa gtcctgtgcg
     2521 cttgtcattt tattcgtatt aacaataaca tctaatccag ctgcaatcgc ggctgttgca
     2581 ccatttacag aatcttcaat agccaaacat tgtgtcggca tataatttaa ttgttgtact
     2641 gcagataaat ataattcagg gtcaggttta acttgttcaa catcttctct accaacaact
     2701 acctctatat aatcgtctag acctaatgct ttaaaggtgg gcattatttc cgctttcaca
     2761 ctacttgtgg caattgccat aggaatgtga cgttgcttta aaaatgccat caatgattta
     2821 atcgtatcaa tcatcggtaa tgttgtacta gtaagatgat gttcttcata aagtttatct
     2881 ttattttccg aaccaatcgc tttaattaaa tggtcatgca aatctgtagc tgctcctcca
     2941 atagaagaac gataaaaatc aatgcttata ggatcggcat tatgcatctc taaatgttta
     3001 ttaataacat taaataaatg ttgttccgta tctattattg ttccatcgaa atcaaatata
     3061 actgctctat acatatattt actcctctat tttttctctt tatcaaagct taacatagtc
     3121 cgatgaccac ttcattctat tagtgcaatt ataacttttt tgaccaatac tttgctaaaa
     3181 taggccctgt tatattatga atgaaactaa acactgcgcc tggtactgct gcaattggat
     3241 taaaatgcaa tgctgctaat gacacagcta aaccagagtt ctgcattcca acttcaatac
     3301 tgactgcttt ttgatcttgt cgatctaatt ttaaaagacg agctaaccaa tatccaatcg
     3361 tataccctaa tacgttatgt aaaataacta ctaaaaatat taataatcct gtagttaaga
     3421 tttggtgctt actgccacct acaactattg ctaaaattaa tgaaatagca acaactgata
     3481 taatcggcaa agctgtagaa gctttttcag caattttacg attaataatt tgcaaaacaa
     3541 tacctaaagc aattggaatt aatacaactt gaacaactga ccacaacata ctcaggaaag
     3601 atacttccaa ccattcattt gcaaatagat atattaacgc aggtgtaacg aatggcgcta
     3661 gcaacgtaga gaccgtcgta atagaaacag aaagtgctac gttagctttg gctaaataac
     3721 tcattacatt acttgatgtc ccacccggac aacatccaac taatattacg ccaacagcaa
     3781 tatcaggtgg taaatgaaaa gactttgcaa ttataaatgc taatgtgggc ataatactga
     3841 attgtagaca gacaccaata attactgctc taggtgcttt aaagaccatt ttgaaatcat
     3901 taggtgtaat tgttaatccc atacctaaca taacaatacc aagtaaataa ggtacccact
     3961 tacctaatgt cgcaacatgt tgtgggaaaa taaatccaat aacagctgct actaacatcc
     4021 ataccaaaaa actcttcgtg gcaaatatac tcaatttttg aaacattgtt aacacatcct
     4081 cgtcgataat attgaaaaat attaccataa gaccagtaag gttacaatta cttttctgaa
     4141 aattaagatt atttagccat tctcttctta ttgcaaatat tttatcactg ataaacaatt
     4201 attttaaact tcattcaaaa gtcttagtga ataaagaaaa aaagcgtggt aaaatcaact
     4261 tgaacaaaat taagtcacct ttataaatat taaacgtgta tttaatgcca tgtattcctc
     4321 gggaggtcct cacatgttaa ttgataaatt cgaaacatac atcatcaaca tcgccggttt
     4381 aaatgaccgt actactagaa aaaaattaag taagttatgc aaaagtgtac aattttgtga
     4441 tgcgttgcaa ttctcaataa acaaacagtt taatcagtat gtattagaaa ttagcttacc
     4501 aaagcaacaa cttccatact tcatttcatt tttaagtttt catcagtata gtattttcca
     4561 agtactttca ccaaagaaaa taaatgaatt acttgattct gataatttat atcaatctgc
     4621 aaagcgtttc gatatcaata ttgatggctt gcaagatgct ttcattaaag acaaagtcat
     4681 cgatattatg aatatgttcc aaaatcacac tgatatcact tatacgttga ataagtctca
     4741 tgcacatatt atatgtacgc cagaaatatt tgctaaattg ttacatacga ttgcaactag
     4801 aaatatcgac attctatctg ccaattatag atcgtcatct atgagcaaag ctcgtatatc
     4861 ataataaaag acacatctat acgatgatca tttacaatga ttgcgtatag tgatgtgtct
     4921 ttttttgtat tatttatctt cggacgattg atcctcaacc atttgttcaa atgcttcacg
     4981 aacttgtggt actgtcatac ctactacaac ttggatactc ttaccactct ttactaatcc
     5041 gtgtgccatt tgttgatgcg tgaaatattc cgtatccgca actttacttt catcataaac
     5101 agttaagcgt aatcttgttg tacagttagt aacatctttg atattttctt tgccgcctaa
     5161 accgtcgagg taatacgcag ctttgtcttc gtattcatta cctgttgaag cacgtttaga
     5221 atctacgcta tcaccttttt tatttttgta atcttgctta gaaaataatt taacttcctc
     5281 ttctgttttc ttacgtccag gtaatggaat atcaaatttc aaaattaaga atctgaataa
     5341 gaagaagtaa atcgctacaa atatcagacc gataataact tgtgcaacat aagtacccca
     5401 atgttcttta cctaatggca accagtttgt tgaaataaag tctaacaagc caccacccat
     5461 gttacctaca acaccaaatg cgtacattaa agtatccatt gatgctgcta aaactgcgtg
     5521 taaaacaaat aagtatggtg cgatgaataa gaatgtaaat tctaatggct cagtaatacc
     5581 tacaacgact gctgttaatg ttgcaggaat aagtaatgct gctacttttt tacgattttc
     5641 tttaggtgtc gtagcataca ttgctaaagc aatacctaaa catccaaata ctttcccatt
     5701 tccttgaagc ataaagccat aagggaattg ctcttttaat ggcttagtac tcttagcaaa
     5761 ctcatttaaa tgttgtaacc attcagcttt caagccatga ttgactacta ctggtccaac
     5821 ttcaattggt gcataaataa agtgatgtaa tcctgttggt attaatacac gttctaagaa
     5881 gtggtacagc caaacaccga tatagcctga agcgatgata aagtgttgca ttgaaccaat
     5941 accgtgctga acagttggcc aaataacaca agtaattgct gctaatggta acatcacaaa
     6001 aaatgaaatt gtaaccacaa atgttaaacc ttggaataca ccaaccattt ctggtaatcg
     6061 tttactgtag tatctattgt gaatccacgt aataatagct gaaataataa taccacctag
     6121 aatattcgta tccaatgtgg caatacctgc aattgatttt aaaccaggta cattttcaac
     6181 gcctttttct aaattagcgc caaatgtatg tggccattgc gttaaaatgg catttataaa
     6241 tgtattaaac attaagtaac ccatcaatgc tgcaagcgct gcatgacctg gtgctttttt
     6301 agctaaagaa agtggtaaac ctacaacaaa tacaacttcc atatgcgtaa aaataaccca
     6361 gccaccagat tctatcactg accaaaattt aaaccaaaac gtatgttgat ctgctaaact
     6421 ccccatgatt gtaggatttt taaataatgt cgcaaagcca agcactatcc caaagaaagc
     6481 gaacattaat accggtacaa tcattgcact accgaaacgc tttatcgcat tcatcttcta
     6541 ttccctccat atcatctttc ctaacaatac atctaattag attcatttat aa
//
LOCUS       DANBWH010000151         6590 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.156, whole genome shotgun sequence.
ACCESSION   DANBWH010000151 DANBWH010000000
VERSION     DANBWH010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6590
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.156"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            25..1527
                     /locus_tag="PPY92_002056"
     CDS             25..1527
                     /locus_tag="PPY92_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDJ2626041.1"
                     /translation="MRVAIIGMGTAGVSVLRELVKHPKFNQLDIDLYDDKVNMGQGVP
                     FQNDSSELLINMPSKKMSLNLDDETEFWKWYKQQTDFNFDEPAYLPRFVFGHYMKSYL
                     SMFTKKYPNISTNYNKVQEIYTNSNIDETNLTYYICTTNSEQSWQAYDYVFLTCGTFA
                     YHDPYNLKGKKGYIATPYPTYNTLDEVNELDDIAIIGTGLASLDVVRYVAAHHPKLPI
                     TMTSRSAHLPSVRGTMIDVTFKYLTKDKLNDIKKHHFGNAPLDTIVSLFLKECAEYDI
                     DFKKLVHRRTGNHIADLKYDLARPTEMGIFQSMIEHLKENLNWIWNSLSIEDQHQFNQ
                     KYSKMIQLNSNPMPPRTAELIIELIENKSLILKKDLEDVKHDGKLYYFSYKNQESVDM
                     YNVVINATGAKSHLNELDQDDQLIKNLENRQIVQAHPMGGIQIIPETNQVISPRFGTL
                     TNMIAIGQMTNGVNKLRNGVKMIVEQVAHTVSQLYDALESNEQQQRSYNQ"
     regulatory      1746..1881
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            2008..3051
                     /gene="ribD"
                     /locus_tag="PPY92_002057"
     CDS             2008..3051
                     /gene="ribD"
                     /locus_tag="PPY92_002057"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDJ2626042.1"
                     /translation="MSQFMDYAIQLANMVQGQTGVNPPVGAVVVNEGRIVGIGAHLRK
                     GDKHAEVQALDMAQQNAEGATIYITLEPCSHFGSTPPCVNKIIDCKIAKVVYATKDNS
                     LDTHGDETLRAHGIEVECVDDERASQLYQDFFKAKAKQLPQITVKVSASLDGKQANDN
                     GQSQWITNKEVKQDVYKLRHRHDAVLTGRRTVELDDPQYTTRIQDGKNPIKVILSKSG
                     NIHFNQQIYQDESTPIWIYTENPNLTSNQTHIEIIYLKSCDLTTILHNLYKRGVGTLL
                     VEAGPTTTSEFLQSNYIDEFILYYAPKLIGGSGNYQFYQTNDVIEIPDANQFEIVHSE
                     LLNQNVKLTLRKK"
     gene            3058..3690
                     /locus_tag="PPY92_002058"
     CDS             3058..3690
                     /locus_tag="PPY92_002058"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDJ2626043.1"
                     /translation="MFTGIVEEIGVVKSVQIRQSVRTIEIEAHKITADMHIGDSISVN
                     GACLTVIDFDQTSFTVQVIKSTENKTYLADVKRQSEVNLERAMSGNGRFGGHFVLGHV
                     DELGTVSKINETANAKIITIQCSQHINNQLVKQGSITVDGVSLTVFDKHDNSFDIHLI
                     PETRRSTILSSKKLGDKVHLETDVLFKYVENILNKDKDQLSVDKLRAFGF"
     gene            3701..4882
                     /gene="ribB"
                     /locus_tag="PPY92_002059"
     CDS             3701..4882
                     /gene="ribB"
                     /locus_tag="PPY92_002059"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HDJ2626044.1"
                     /translation="MQFDNIDSALMALKNGETIIVVDDENRENEGDLVAVTEWMNDNT
                     INFMAKEARGLICAPVSKDIAQRLDLVQMVDDNSDIFGTQFTVSIDHVDTTTGISAYE
                     RTLTAKKLIDPSSEAKDFNRPGHLFPLVAQDKGVLARNGHTEAAVDLAKLTGAKPAGV
                     ICEIMNDDGTMAKGQDLQNFKEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFG
                     TFDMYGFKATYTDEEIVVLTKGAIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMK
                     YINEHGGMIIYLPQEGRGIGLLNKLRAYELIEQGYDTVTANLALGFDEDLRDYHIAAQ
                     ILKYFNIEHINLLSNNPSKFEGLKQYGIDIAERIEVIVPETVHNHDYMVTKKIKMGHL
                     I"
     gene            4895..5359
                     /locus_tag="PPY92_002060"
     CDS             4895..5359
                     /locus_tag="PPY92_002060"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDJ2626045.1"
                     /translation="MNFEGKLIGKDLKVAIVVSRFNDFITGRLLEGAKDTLIRHDVNE
                     DNIDVAFVPGAFEIPLVAKKLASSGNYDAIITLGCVIRGATSHYDYVCNEVAKGVSKV
                     NDQTNVPVIFGILTTESIEQAVERAGTKAGNKGAEAAVSAIEMANLLKSIKA"
     gene            complement(5481..6482)
                     /locus_tag="PPY92_002061"
     CDS             complement(5481..6482)
                     /locus_tag="PPY92_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase"
                     /protein_id="HDJ2626046.1"
                     /translation="MALLKNFFIGLSNNSFLNNAAKKVGPRLGANKVVAGNTIPELIN
                     TIEYLNDKNIAVTVDNLGEFVGTVEESNHAKEQILTIMDALHQHGVKAHMSVKLSQLG
                     AEFDLELAYQNLREILLKANTYNNMHINIDTEKYASLQQIVQVLDRLKGEFRNVGTVI
                     QAYLYDSHKLVDKYQDLRLRLVKGAYKENESIAFQSKEDVDANYIKIIEQRLLNARNF
                     TSIATHDHRIINHVKQFMKENHIEKDRMEFQMLYGFRSELAEEIANEGYNFTIYVPYG
                     DDWFAYFMRRLAERPQNLSLAVKEFVKPAGLKRVGIIAALGATVMLGLSTIKKLCRK"
BASE COUNT         2344 a          951 c         1216 g         2079 t
ORIGIN      
        1 gaattacaaa aggatggatg atatatgcgt gtagcaataa taggcatggg aactgctggt
       61 gtaagtgtat tgagagagtt agttaagcac ccgaagttta atcaattaga tatagattta
      121 tacgatgata aagtaaatat gggacagggc gtaccatttc aaaatgatag ttctgagctg
      181 ctaattaata tgccttcgaa aaagatgagt ttaaatttag atgatgagac tgaattttgg
      241 aagtggtata aacaacaaac tgattttaat tttgatgaac ctgcatattt accgagattt
      301 gtattcggac attatatgaa atcttattta tctatgttca cgaaaaaata tccaaatata
      361 tcaactaatt ataataaagt tcaagagatt tatacaaatt ctaatatcga tgaaacgaat
      421 ttaacttatt acatatgtac tacaaattca gaacaatcat ggcaagcgta cgattatgta
      481 tttttaacat gtggtacatt tgcctaccat gatccatata acttaaaggg taaaaaagga
      541 tatatcgcta cgccttatcc aacatataat acgttagacg aagtaaatga actggacgat
      601 attgcgatta ttggtacagg ccttgccagt ttagatgttg tccgttatgt tgctgctcat
      661 catccgaaat taccaattac tatgacgagt cgttctgctc atttaccaag tgtaagaggt
      721 acaatgatag acgtgacatt caagtactta actaaagaca aattaaatga cattaaaaaa
      781 catcatttcg gcaatgcacc gcttgatact atagtttctt tatttttaaa agaatgtgct
      841 gaatatgaca ttgattttaa aaaattagta catagacgta caggaaatca tattgcagat
      901 ttgaagtatg atttagcgcg tccaacagaa atgggcattt tccaaagtat gattgaacat
      961 ttgaaagaga atttaaattg gatatggaat agcttaagta ttgaagatca acatcaattt
     1021 aatcaaaaat actcaaagat gattcaatta aattcaaatc caatgcctcc aagaactgct
     1081 gaactaatta ttgaattaat agaaaataag tctttaattt tgaaaaaaga tttagaagat
     1141 gtgaaacatg atggcaagtt gtattatttt tcatacaaaa accaagaatc agtagacatg
     1201 tataatgttg tcattaacgc gacaggtgca aaaagtcatt taaatgaatt ggatcaagat
     1261 gatcaattaa ttaaaaatct tgaaaataga caaattgtac aagcacatcc aatgggaggc
     1321 atacaaatca taccggaaac caatcaagtt attagtccaa ggtttggaac attaacaaat
     1381 atgattgcga taggtcaaat gacaaacggt gttaataaac tacgaaatgg tgtgaaaatg
     1441 atagtagaac aagttgcgca tacagtatct caattatatg atgctttaga atcgaatgag
     1501 caacaacagc gcagttacaa tcaataattt gtaactagaa gataataaag agaacgctct
     1561 atagagacga attgaaggtt tgattttaat gtctgttagt aagaatcata tcaatgagat
     1621 gcctatagta ctcagattat attaaattaa aaccgtcatt aattgttttt tcagaaaaca
     1681 tatagtatca ttttaaatgt agttgacata ctacgtactc aaataatcta taacaatttc
     1741 atatataatt ctttcggggc agggtgaaat tcccaaccgg cagtaaataa agcctgcgac
     1801 ctgctaatat gtttcatatt agtggctgat ctagtgagat tctagagccg acagttaaag
     1861 tctggatggg agaaagaatg ttaattatcg acaaagataa tgtagcgtat ttgtaaaaat
     1921 gtgtacaaat aggcttattt aacgataaat ttttctcctt gcatcttaat tcatgatgtg
     1981 aggatttttg tttatagagg tgatcatttg agtcaattta tggattatgc gattcaactt
     2041 gcaaatatgg tacaaggtca aacaggtgtt aatccacccg ttggcgctgt tgtagttaat
     2101 gaaggtagga ttgttggtat tggtgcacac ttgagaaaag gtgacaagca tgcggaggtt
     2161 caagcacttg atatggcaca acaaaatgct gaaggtgcga cgatttatat tacgttagag
     2221 ccatgtagtc attttggttc aacaccaccc tgtgttaaca aaattattga ttgtaagata
     2281 gcaaaagtag tatacgcaac aaaagacaat tcgttagaca cacatggtga tgagacgtta
     2341 cgggctcacg gtattgaggt tgaatgcgtt gatgatgaac gggcatcaca attataccaa
     2401 gactttttta aagcaaaagc aaagcaactg ccacaaatta cagtgaaagt atctgcaagt
     2461 ttagatggta aacaagcgaa tgataatgga caaagtcaat ggattactaa caaagaggtt
     2521 aaacaagatg tctataagtt aagacatcga cacgacgcag tgttaactgg aagacgtaca
     2581 gttgaattag atgatccaca atatactaca cgtattcaag atggaaaaaa ccctataaaa
     2641 gtaatattgt ctaagtctgg gaatattcat tttaatcagc aaatttatca agatgaatca
     2701 acaccaattt ggatatatac tgaaaatcca aatttaacaa gcaatcaaac acatattgaa
     2761 attatttact tgaagtcttg tgatttaaca acaattcttc acaatttata taaaagagga
     2821 gttggaactt tgctagtcga ggcaggtcca accactactt cagaatttct ccaatctaat
     2881 tatatagatg aatttattct ctattatgcc ccgaaattaa ttggcggatc tggaaattat
     2941 caattttatc aaacaaatga tgtgattgag ataccagatg cgaaccaatt tgaaattgtt
     3001 cattccgagt tattaaatca aaatgttaaa ttaactttac gaaagaagtg atgatgcatg
     3061 tttactggca tcgttgaaga aataggtgtc gttaaaagtg ttcaaattcg tcaatcagtc
     3121 aggacgattg aaatcgaagc acataagatt acggcagata tgcatattgg tgattccatc
     3181 agtgttaatg gtgcatgttt aacagtgatt gattttgatc agacatcttt tactgttcaa
     3241 gtaattaaaa gcactgaaaa taaaacctat ttagcagatg ttaagcgaca atcagaagta
     3301 aatttagagc gtgccatgag tggtaacggt aggtttggtg gacattttgt cctcggtcat
     3361 gtagatgaac taggaacagt ttcaaaaata aatgaaacag ccaatgccaa aattattacg
     3421 attcaatgta gccaacatat taataatcag ttagttaagc aagggtctat tactgtggat
     3481 ggtgtaagtc taacggtatt tgataagcat gataacagtt ttgacattca tcttattcca
     3541 gaaacgaggc gttcaacgat tttatcatcc aaaaaattag gagataaagt acatttagaa
     3601 acagacgttt tgtttaaata tgttgaaaat attttaaata aagataaaga ccaattatct
     3661 gtagataaat taagagcatt tggtttttag gaggggtagc atgcaattcg ataatattga
     3721 cagtgcttta atggctttaa aaaatggaga aacaattatt gtagtagatg atgagaatcg
     3781 tgaaaatgaa ggtgatttag tagcggttac tgaatggatg aacgataata ccattaattt
     3841 tatggcgaaa gaagcaaggg gattaatatg cgcaccagtg tctaaagata ttgcacaacg
     3901 tttggatttg gtacaaatgg ttgatgataa ctccgacatc tttggtacgc aatttacagt
     3961 gagtattgat catgtagata caacaacagg aattagtgct tatgaacgta cattgactgc
     4021 caaaaagctc attgatccta gtagtgaagc taaagatttt aatcgtcctg gtcatttatt
     4081 tccattagta gcacaagata aaggcgtatt agctagaaat ggacacacag aagcggctgt
     4141 tgatttagct aaacttactg gtgccaagcc cgctggtgtc atttgtgaga ttatgaatga
     4201 tgacggcacg atggcgaaag gacaagattt acaaaatttt aaagaaaaac atcaattaaa
     4261 gatgattacg attgatgatt taattgaata tcgtaaaaaa ttagaaccag aaattgaatt
     4321 taaggcaaaa gtgaaaatgc ctacagattt cggaacattt gatatgtatg gttttaaagc
     4381 gacatacaca gatgaagaga tagttgtact gacaaaaggt gcaattcgac aacatgaaaa
     4441 tgtacgctta cattctgcgt gccttacagg cgatattttc catagtcaac gttgtgattg
     4501 tggtgctcaa cttgaatcgt ctatgaagta tatcaatgaa catggtggca tgattattta
     4561 tctacctcaa gaaggtcgtg gcataggatt gttaaacaaa ttacgcgcat atgaattaat
     4621 tgagcaagga tatgatacag taactgcaaa tttagcatta ggttttgatg aagatttacg
     4681 agattatcat attgctgcac agattttaaa atattttaac atcgaacata tcaatttatt
     4741 aagtaataat ccaagtaaat ttgagggatt aaaacaatat ggcattgata ttgcagaaag
     4801 aattgaagtt atcgtaccag aaacggtaca taatcatgat tatatggtaa cgaaaaaaat
     4861 aaaaatgggt catttaatat aggaggactt taacatgaat tttgaaggta aattaattgg
     4921 aaaagatttg aaagttgcaa tcgtagttag tcgatttaat gattttatca ctggaagatt
     4981 acttgaaggt gcaaaagata ctttgattcg acatgatgtt aatgaagaca atattgatgt
     5041 agcatttgtt cctggtgcgt ttgaaattcc tttagtagct aaaaaattag cctcatcagg
     5101 aaattatgat gcaataatta cattaggatg cgtaattcgc ggtgctacgt ctcattatga
     5161 ttatgtttgt aatgaagtcg cgaaaggtgt ttctaaagta aatgatcaaa ctaatgtacc
     5221 agtcatattt ggtattttaa cgactgaaag tattgaacaa gctgtggaaa gagcaggtac
     5281 gaaagctggt aataaaggtg ccgaagcagc agtaagtgca attgaaatgg ctaatttatt
     5341 aaaatctata aaagcataga gcgctgttgt gagttcataa tcaaagattt ataaatgttt
     5401 cagaactgcc aatataacat ttaggagcct agaacattga ttattgttcc agactcctaa
     5461 attattgttt atgccatgct ctatttacgg cataattttt taattgtact taaacctaac
     5521 ataactgtag ctcctaaagc tgcaattatg ccaacacgtt ttaagccagc aggtttcaca
     5581 aattctttta cagcaagaga taggttttgt gggcgttctg ctaatcttct cataaaatac
     5641 gcaaaccaat catcgccata aggtacataa atagtgaaat tatagccttc atttgcgatt
     5701 tcttctgcta actctgatct aaaaccatag agcatttgga attccatacg atctttttca
     5761 atgtgatttt ctttcataaa ttgttttaca tgattaatga tgcgatggtc atgtgttgca
     5821 attgaagtga aattgcgtgc gtttaacaaa cgttgttcaa ttattttgat gtaatttgca
     5881 tctacgtctt ccttagattg aaatgcaatt gattcgtttt ctttatatgc acctttaacc
     5941 aaacgtaatc gtaaatcttg gtacttatca actaatttgt ggctatcgta taaatatgct
     6001 tgaattacag taccaacatt tctaaattcg ccttttaagc gatctaaaac ttgaacaatt
     6061 tgttgcaggc tagcatattt ttcagtatca atatttatat gcatattgtt gtaagtattt
     6121 gctttaagta aaatttctct taaattttgg taagctaatt ctaagtcgaa ttctgcacct
     6181 aactgactca atttaacaga catatgtgcc tttacgccat gttgatgaag cgcgtccata
     6241 attgttaaaa tttgttcttt agcatgatta ctttcttcaa ctgtaccgac aaattcccct
     6301 aaattgtcta ccgtaacagc gatattcttg tcatttaagt attcgattgt attaattaac
     6361 tctggaattg tatttccggc aacgacttta ttggcgccca aacgtgggcc cacttttttt
     6421 gctgcgttgt ttaaaaaact attattagat aatccgataa aaaaattctt taatagtgcc
     6481 attgctactc ctccaaaccc ttgtgtttac aataagttta gcatgaaaaa aatattcgag
     6541 aaagcgtttt cataaaaaac aatcaaaaat aattatgtat aattacacta
//
LOCUS       DANBWH010000152         6570 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.157, whole genome shotgun sequence.
ACCESSION   DANBWH010000152 DANBWH010000000
VERSION     DANBWH010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.157"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            58..558
                     /gene="ftnA"
                     /locus_tag="PPY92_002062"
     CDS             58..558
                     /gene="ftnA"
                     /locus_tag="PPY92_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-type ferritin FtnA"
                     /protein_id="HDJ2626047.1"
                     /translation="MLSKNLLEALNDQMNHEYFAAHAYMAMAAYCDKESYEGFANFFI
                     QQAKEERFHGQKIYNYINDRGAHAEFRAVSAPKIDFSSILETFKDSLSQEQEVTRRFY
                     NLSEIARQDKDYATISFLNWFLDEQVEEESMFETHINYLTRIGDDSNALYLYEKELGA
                     RTFDEE"
     gene            complement(640..732)
                     /locus_tag="PPY92_002063"
     CDS             complement(640..732)
                     /locus_tag="PPY92_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626048.1"
                     /translation="MTKNTIISLENEKTQINDSENESSDLRKAK"
     gene            948..1502
                     /locus_tag="PPY92_002064"
     CDS             948..1502
                     /locus_tag="PPY92_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HDJ2626049.1"
                     /translation="MIQDAFVALDFETANGKRTSICSVGMVKVIDSQITETFHTLVNP
                     QDYFSQQNIKVHGIQPEDVENAPTFDYVFPYMMQFIADLPVVAHNAAFDMNVLHQSIQ
                     NIGLPTPNLTYFCSYQLAKRTVDSYRYGLKHMMEFYQLDFHGHHDALNDAKACAMITF
                     RLLKNYENLTYVTNIYGKNLKDKG"
     gene            complement(1570..2640)
                     /gene="dinB"
                     /locus_tag="PPY92_002065"
     CDS             complement(1570..2640)
                     /gene="dinB"
                     /locus_tag="PPY92_002065"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDJ2626050.1"
                     /translation="MTERRIIHIDMDYFFAQVEMRDNPKLKGKPVIVGGKASSRGVVS
                     TASYEARKYGVHSAMPMSQAHKLCPNGYFVTSNFGAYRETSAQIMSIFRSYTDKVEPM
                     SLDEAYLDITELVRPDLPASKIAQYIRKDILEQTHLTASAGVSYNKFLAKLASGMNKP
                     DGLTVIDYQNVHDILMTLDIGDFPGVGKASKKVMHDNGIFNGRDLYEKTEFELIRLFG
                     KRGRGLYNKARGIDHSEVKSSRVRKSVGTERTFATDVNDDEEILRKVWELSGKTAERL
                     NKLQKSAKTVTVKIKTYQFETLSKQMSLRDSVSSEEDIYNIAYLLYNDLKDPDVPIRL
                     IGVTVGNLEQSTYKNMTIYDFI"
     gene            complement(2887..3417)
                     /locus_tag="PPY92_002066"
     CDS             complement(2887..3417)
                     /locus_tag="PPY92_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HDJ2626051.1"
                     /translation="MHKIDLTTNNFQMRRFIILQLVIALFVILFTYKWALGVTAVVDQ
                     NIIINLVYGFAGFIILLILHELIHRALFLLFKKDSKPMFNIKKDRILFQTADACFNKW
                     QFSIIMLSPLILLSTGLLILIKVFGYSSLIFMFSMHTAYCFIDILLVALTISSSFKYV
                     QQDEDSIYLYHQKPTQ"
     gene            complement(3587..4948)
                     /gene="rlmD"
                     /locus_tag="PPY92_002067"
     CDS             complement(3587..4948)
                     /gene="rlmD"
                     /locus_tag="PPY92_002067"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDJ2626052.1"
                     /translation="MQAIAKNDIKTGTVVDLTHEGHGVVKIDRFPIFIPQALINEQIE
                     YKIIKVKKNFAIGKLLNINTRSENRVAPPCIYYERCGGCQLQHLSYEAQLEMKKEQVI
                     NLFQRKAHFDNSKINDTVGMTDPWRYRNKSQIPVGKNEQNEVIMGFYRQRSHDIIDME
                     SCLIQDSQHQEVMNEVKSILKDLNVSIYQEQLKKGLMRHLVVRTGYHTDEMMIIFVTN
                     GKKWPQKNAVVEKILDAFPNVTSIKQNINDSHSNVIMGRQSITLYGKDTIIDQLTDST
                     FKISDQSFYQINSEQTEKLYNKAIEYAQLTGNEVVLDTYCGIGTIGLYMAPHAKHVYG
                     VEVVPSSIEDAQQNATINQCNNTTFVCGKAEEVILQWKAQGIKPDVVMVDPPRKGCDE
                     TFIQTLLTLEPKRIVYISCNPATQQRDALLLAEKYQLEEVTPVDMFPQTTHVETVALF
                     NLK"
     gene            complement(5029..5976)
                     /locus_tag="PPY92_002068"
     CDS             complement(5029..5976)
                     /locus_tag="PPY92_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDJ2626053.1"
                     /translation="MRKRARIIYNPTSGKELFKRELPDALIKLEKAGYETSAYATEKI
                     GDATLEAERAMHENYDVLIAAGGDGTLNEVVNGIAEKPNRPKLGVIPMGTVNDFGRAL
                     HIPNDIMGALDVIIEGHSTKVDIGKMNNRYFINLAAGGQLTQVSYETPSKLKSIVGPF
                     AYYIKGFEMLPQMKAVDLRIEYDGNVFQGEALLFFLGLTNSMAGFEKLVPDAKLDDGY
                     FTLIIVEKSNLAELGHIMTLASRGEHTKHPKVIYEKAKAINISSFTDLQLNVDGEYGG
                     KLPANFLNLERHIDVFAPNDIVNEELINNDHVDDNLIEE"
BASE COUNT         2124 a         1186 c          928 g         2332 t
ORIGIN      
        1 tgttatacta agtttaattc atagtaattt taatttacaa ataagaggtg tatcaaaatg
       61 ttaagtaaaa atttattaga agcattaaat gatcaaatga accatgagta ctttgcagca
      121 cacgcatata tggcaatggc agcatactgt gataaagaat cgtacgaagg atttgcaaac
      181 ttcttcattc aacaagctaa agaagaacgt ttccatggac aaaagattta taactatatt
      241 aacgacagag gtgcacatgc agaattcaga gcagtttcag caccaaaaat tgacttttca
      301 agcatactag aaactttcaa agacagctta tctcaagaac aagaagtaac aagacgtttc
      361 tataacttat ctgaaatcgc tcgtcaagat aaagattatg caactatctc attcttaaac
      421 tggttcttag atgaacaagt cgaagaagaa tcaatgtttg aaactcacat caattattta
      481 actcgtatcg gcgatgacag caatgcatta tatctttacg aaaaagaact tggcgctcgt
      541 acattcgacg aagaataatt aaacatcact acaatagaca gataaatatc atacgacatg
      601 ataggcattt gggtcactta caataaccca atgtctatat tattttgctt tacggagatc
      661 actagattca ttttctgaat cattgatctg cgttttttca ttttcaaggc taattattgt
      721 atttttagtc atttattttt taaactacta atgttaataa ctctaaattt gatgttgaat
      781 taatttgacc gattttaaag catatcatca tttacttttt aatcagagtt acatccaaat
      841 gatagatttc acgttatacc ttcacgtata atattatgta tcgtttgtaa gcaaatgact
      901 aaaagtctat taatatatac atttaattaa ttgaaaggat tgactacatg atacaagatg
      961 cgtttgttgc acttgatttt gaaacagcaa atggtaaacg tacaagtatt tgttctgtcg
     1021 gaatggttaa agtcattgat agtcaaataa cagaaacatt tcatactctt gtgaatccgc
     1081 aagactattt ttcacaacaa aatattaaag ttcatggcat acaaccagaa gatgttgaaa
     1141 atgcacctac gtttgactac gtatttccat atatgatgca atttattgca gatttacctg
     1201 ttgtcgcaca taacgcggca tttgatatga acgtcttaca tcaaagcatt caaaatattg
     1261 gtttaccaac tccaaattta acttactttt gtagttatca acttgctaaa agaaccgttg
     1321 attcgtatcg atacggttta aaacatatga tggagtttta tcaattagat tttcatggtc
     1381 atcatgatgc attgaatgat gccaaagcat gcgcaatgat tacttttagg ctactgaaaa
     1441 attatgaaaa tttaacatat gtaactaata tttatggtaa aaatctaaaa gataaaggct
     1501 aggactaaat aaaatactcc cttcaaaagt aagcattgta aaaatgtaaa ctttgcaggg
     1561 agctttattt tatataaagt catatatcgt catattttta taagttgatt gttctaaatt
     1621 acctacagtg acaccaataa gtcgaattgg tacatctggg tcttttaaat cgttataaag
     1681 taaatatgca atattataaa tatcttcttc agaactaacc gaatctctta aactcatctg
     1741 tttagatagc gtttcaaatt gataagtttt aattttaacc gttacagttt tagctgactt
     1801 ctgtaattta tttagacgtt cagctgtttt acctgacaat tcccatactt ttcttaaaat
     1861 ctcttcatca tcattcacgt ctgttgcaaa tgtgcgttca gtccctactg attttcttac
     1921 tcttgatgat ttcacttcac tatggtcaat accgcgtgcc ttgttatata aaccccgacc
     1981 tctttttcca aacaaacgta ttaattcaaa ttccgttttc tcatataaat ctctaccgtt
     2041 aaaaatacca ttatcatgca ttactttttt ggaagcttta cctacgcctg gaaaatctcc
     2101 aatatccaat gtcatcaaaa tatcatggac attttgataa tcaatcacag tcaaaccatc
     2161 aggtttattc ataccactcg ctaatttagc taaaaatttg ttataagaaa cacctgcaga
     2221 tgctgttaaa tgtgtctgct ctagaatatc ttttctaata tactgagcaa ttttcgaagc
     2281 aggaaggtct ggtctcacta attctgtaat atctaaatac gcttcatcca atgacatcgg
     2341 ttctacctta tctgtataac ttcggaaaat agacataatc tgcgcagatg tttctcggta
     2401 agcaccaaaa ttacttgtga caaagtatcc atttggacat aatttatgcg cttgtgacat
     2461 aggcattgct gaatggacgc cgtattttcg tgcttcatag gatgccgtag agacaacacc
     2521 cctactgctt gctttaccac caacaatgac tggtttccct ttcaatttgg ggttatctct
     2581 catttcgact tgtgcaaaaa aatagtccat atctatatga ataattcgtc tctcagtcaa
     2641 gtgctcacct ccctactaat ttttactttt ataacgcaca aaaatatctc aacataatta
     2701 tacgctgtgt acgatttttt tacataaatc ttgcacttag cgataactat attgagataa
     2761 ctacaagttg ttataaaatc aattgctatt taagcatgat gatgaagacg attgagtaag
     2821 aaaacatagg taatctgaaa taattcaagc aaattcattt tgttggtatt atcatattaa
     2881 aatttattat tgagtcggct tttgatgata caaataaata ctatcttcat cttgttgaac
     2941 atatttaaaa ctactagata ttgttaatgc aacaagcaga atatctataa agcaataagc
     3001 ggtatgcata ctaaacataa aaataagtga tgaataacca aataccttaa ttaaaataag
     3061 caagccagta cttaataaga ttagtggtga cagcataata attgagaatt gccatttgtt
     3121 gaagcaagca tctgctgttt ggaataagat tctgtctttt tttatattaa acataggttt
     3181 gctatctttt ttaaataaaa gaaataatgc tctatggata agttcatgta aaatcaataa
     3241 aataatgaat ccagcaaacc catatacaag attgatgatg atattttgat cgacaaccgc
     3301 tgtgacacct aacgcccact tatacgtaaa taaaatcacg aataacgcaa taacaagttg
     3361 caagataata aaccttcgca tttgaaaatt atttgtcgtt aaatcaattt tatgcattac
     3421 caaccctccc gatcatgaca ttcttattct tctttaaata tagtatacaa tgtcacattt
     3481 aatttaaaaa gttcatatca agaaagtaaa ttggctgtaa taaaatttta atatacgact
     3541 tctttcttca cttattaagg cgaaatttta tctcaaatca tgtgcgctat ttcaaattga
     3601 ataatgccac tgtctcaaca tgtgttgttt gtggaaacat atctaccggt gttacctctt
     3661 caagttgata tttttcagct aataataatg catcacgttg ctgtgttgcg ggattacatg
     3721 aaatatagac aatacgctta ggttctaatg taagcaaagt ctgaataaac gtttcgtcac
     3781 agccctttct tggcggatca accattacaa catctggttt aatcccttgt gctttccatt
     3841 gtaaaataac ttcttcagct ttcccacaga caaaagttgt attattgcat tggtttatag
     3901 tcgcattttg ttgtgcgtct tcaattgaag aaggtactac ttcaacaccg tatacatgtt
     3961 ttgcatgtgg tgccatatat agccctattg ttccaatacc acaataggta tctaatacaa
     4021 cttcattacc tgtcaattgc gcatactcaa ttgctttatt atataatttc tctgtttgtt
     4081 cagaattaat ttggtagaat gactgatcac ttattttaaa tgtactatct gttaattgat
     4141 caataattgt atctttacca tatagcgtta tagattgacg tcccataata acattagagt
     4201 ggctatcatt aatgttttgt ttaatgcttg tcacattagg aaatgcatct aatatcttct
     4261 caacaacagc atttttttgt ggccactttt taccattagt tacaaaaata atcatcattt
     4321 cgtctgtatg atatcctgtt cttacaacca aatgtctcat taaacctttt ttcaattgtt
     4381 cttgataaat acttacattt aaatctttta aaatagattt aacttcattc atcacttctt
     4441 gatgttgtga atcttgtatt aaacaacttt ccatgtcaat aatgtcatgg cttctttgac
     4501 gataaaagcc cataataact tcattctgtt cattcttacc aactggaatc tgggacttgt
     4561 ttcgatatct ccaaggatct gtcatgccaa ctgtatcgtt aatcttagaa ttatcaaaat
     4621 gcgcttttcg ctgaaacaaa ttaatcactt gttccttttt catttcaagt tgtgcttcgt
     4681 atgataagtg ttgaagttgg cacccaccac aacgttcata atatatacat ggcggtgcaa
     4741 cacgattctc acttctcgta tttatattaa gcaacttacc tatcgcaaag ttctttttta
     4801 ctttgataat tttatattca atttgttcat taattaaagc ttgtggtatg aaaataggaa
     4861 agcgatctat ttttacaaca ccatggcctt catgcgttaa atcaacaact gttcccgttt
     4921 ttatgtcatt tttagctatt gcttgcaaaa ttttacctcc aaaatgaaca ggttaggaac
     4981 aaaattatgc gcttcctaac ctgccattat atatttcact atttctgttt attcttcgat
     5041 taaattgtca tcaacatgat cattatttat taactcttca tttacaatat cattaggtgc
     5101 aaagacatct atatgacgtt ctagatttaa gaaattcgct ggtaatttac caccatattc
     5161 tccatctaca tttagttgta agtctgtgaa tgatgaaata ttaattgcct ttgctttttc
     5221 ataaataact ttaggatgct tagtatgttc tcctcttgaa gctaaagtca taatatgacc
     5281 aagttctgca aggtttgatt tttcaactat aattaacgta aaatagccgt catctaactt
     5341 agcgtccggc actaattttt caaatcctgc cattgaattt gttaaaccta aaaagaataa
     5401 taatgcttct ccttggaaaa cattaccatc atattcaatt cttaaatcta cagctttcat
     5461 ttgaggtaac atttcgaaac ctttgatgta ataagcaaat ggaccaacaa tagatttcaa
     5521 tttactcggt gtttcataag agacttgcgt caattgtccg cctgcagcta aattaataaa
     5581 gtatcgatta ttcattttac caatatctac tttagtagaa tgaccttcaa tgatgacatc
     5641 aagtgccccc atgatgtcat taggtatatg caatgcacgt ccaaagtcat taacagtacc
     5701 cataggaatg acacctagct taggacgatt aggcttttct gcgataccat taactacttc
     5761 atttaatgtt ccatcaccac ctgcagcgat taatacatca taattttcat gcatagctct
     5821 ttctgcttca agtgtggcat cacctatttt ctcggttgca tatgcactcg tttcatatcc
     5881 cgctttttct aattttatta aggcatcagg taattctctt ttaaatagct ctttacctga
     5941 tgtcgggtta taaatgattc tagcacgttt cctcatatct tatccctcta cttaaaattc
     6001 atatatttta acttcatctt tgtttcgtct aatagggagt gggacagaaa taatatttaa
     6061 caaaatttat ttcgttctac cccaacttgc attgtctgta gaatttcctt tcgaaattct
     6121 ctttgtttgg ggccccaccc caacttgcac attattgtaa gctgacagaa agtcagcttc
     6181 tatgttgggg ccccgccaac ttgcacatta ttgtaagctg acagaaagtc agcttctatg
     6241 ttggggcccc actagaattg aaaaaagctt gttacaagcg tattttcttt cagtcaacta
     6301 ctgccaatat aacattgtag tgcctaggac attgaattta tgacccaggc tcagtcttat
     6361 ttcatcattc ttaatatcgt taaagaccaa cttgtatctt aaacaaatac tatctcaata
     6421 tgtacaaagc ttgttattta ttcagcattt tttgccgttc ttcattatat agacttcgtc
     6481 agttatgcta ttttaccttt aaaatgatgt tgtaaatata atgttgtctt tatacaaaaa
     6541 tgttttatac ctttttatta tacagtttgc
//
LOCUS       DANBWH010000153         6436 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.158, whole genome shotgun sequence.
ACCESSION   DANBWH010000153 DANBWH010000000
VERSION     DANBWH010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6436
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.158"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            147..1706
                     /gene="rny"
                     /locus_tag="PPY92_002069"
     CDS             147..1706
                     /gene="rny"
                     /locus_tag="PPY92_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDJ2626054.1"
                     /translation="MNLLSLLLILLGIILGVVGGYVVARNLLLQKQSQARQTAEDIVN
                     QAHKEADNIKKEKLLEAKEENQILREQTEAELRERRSELQRQETRLLQKEENLERKSD
                     LLDKKDEILEQKESKIEEKQQQVDAKESSVQTLIMKHEQELERISGLTQEEAINEQLQ
                     RVEEELSQDIAVLVKEKEKEAKEKVDKTAKELLATAVQRLAADHTSESTVSVVNLPND
                     EMKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARTALVNLVSDG
                     RIHPGRIEDMVEKARKEVDDIIREAGEQATFEVNAHNMHPDLVKIVGRLNYRTSYGQN
                     VLKHSIEVAHLASMLAAELGEDETLAKRAGLLHDVGKAIDHEVEGSHVEIGVELAKKY
                     GENETVINAIHSHHGDVEPTSIISILVAAADALSAARPGARKETLENYIRRLERLETL
                     SESYDGVEKAFAIQAGREIRVIVSPEEIDDLKSYRLARDIKNQIEDELQYPGHIKVTV
                     VRETRAVEYAK"
     gene            complement(2003..2218)
                     /locus_tag="PPY92_002070"
     CDS             complement(2003..2218)
                     /locus_tag="PPY92_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626055.1"
                     /translation="MSINIDPEKFAELVVMSNPSKFEDAEDIAKESLKLYINAYRLAE
                     RYSTIATNCYDTAEVIKELKKTDLQLK"
     gene            2392..3189
                     /locus_tag="PPY92_002071"
     CDS             2392..3189
                     /locus_tag="PPY92_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HDJ2626056.1"
                     /translation="MRIMFIGDIVGKIGRDAIETYIPQLKQKYKPTVTIVNAENAAHG
                     KGLTEKIYKQLLRNGVDFMTMGNHTYGQREIYDFIDEAKRLVRPANFPDEAPGIGMRF
                     IQINDIKLAVINLQGRAFMPDIDDPFKKADQLVKEAQEQTPFIFVDFHAETTSEKYAM
                     GWHLDGRASAVVGTHTHIQTADERILPKGTGYITDVGMTGFYDGILGINKTEVIERFI
                     TSLPQRHVVPNEGRSVLSGVVIDLDKEGKTKHIERILINDDHPFSTF"
     gene            3329..5089
                     /locus_tag="PPY92_002072"
     CDS             3329..5089
                     /locus_tag="PPY92_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HDJ2626057.1"
                     /translation="MKPQLSWKVGGQQGEGIESTGEIFATAMNRKGYYLYGYRHFSSR
                     IKGGHTNNKIRVSTTPVHAISDDLDILIAFDQETIDVNHHEMREDSIILADAKAKPVK
                     PEGCHAQLIELPFTATAKELGTALMKNMVAIGATSALMNLNTNTFEELITNMFSKKGD
                     KVVEVNIQALNEGYQLMQSRLPEIDGDFELESTDALPHLYMIGNDAIGLGAIAAGSQF
                     MAAYPITPASEVMEYMIANISKVNGAVIQTEDEIAAVTMAIGANYGGVRAFTASAGPG
                     LSLMMEAIGLSGMTETPLVIINTQRGGPSTGLPTKQEQSDLMQMIYGTHGDIPKIVVA
                     PTDAEDAFYLTMEAFNLAEQYQCPVIVLSDLQLSLGKQTVEKLDYNRIEIKRGEIIQS
                     DIEREEDDKGYFKRYALTSNGVSPRPIPGVKGGIHHITGVEHNEEGKPSESASNRQQQ
                     MEKRMRKIEQLLIESPVEANLQHEDADILYIGFISTKGAIQEGSNRLNQQGIKVNTIQ
                     IRQLHPFPTSVIQDAVNKAKKVVVVEHNYQGQLASIIKMNVNIHDKIENYTKYDGTPF
                     LPHEIEEKGKIIATEIKEMV"
     gene            5090..5956
                     /locus_tag="PPY92_002073"
     CDS             5090..5956
                     /locus_tag="PPY92_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HDJ2626058.1"
                     /translation="MATFKDFRNNVKPNWCPGCGDFSVQAAIQKAAANIGLEPEEVAI
                     ITGIGCSGRLSGYINSYGVHSIHGRALPLAQGVKMANKDLTVIASGGDGDGYAIGMGH
                     TIHALRRNMNMTYIVMDNQIYGLTKGQTSPSSAVGFVTKTTPKGNIEKNVAPLELALS
                     SGATFVAQGFSSDIKGLTKLIEDAINHDGFSFVNVFSPCVTYNKINTYDWFKEHLTSV
                     DDIENYDSTDKQLATKTVIEHESLVTGIVYQDKETPSYESQIKELDDTPLAKRDIKIT
                     EDTFNALTEQFI"
     gene            6050..6343
                     /locus_tag="PPY92_002074"
     CDS             6050..6343
                     /locus_tag="PPY92_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HDJ2626059.1"
                     /translation="MKDTLMSIQIIPKTPNNDNVIPYVDEAIKIIDESGLHFRVGPLE
                     TTVQGNMNECLILIQSLNERMVELECPSIISQVKFYHVPDGITIETLTEKYDE"
BASE COUNT         2372 a          923 c         1222 g         1919 t
ORIGIN      
        1 attcaatata gtgcaaaagt gcaaattgat aacttgacac ttatctccta taaaccgtac
       61 aattaatttg tatgatttat atataatttc ataaagtcat attgaatttc atataaagag
      121 caaaccctag aaaaggaggt gtttgtgtga atttattaag cctcctactc attttgctgg
      181 ggatcattct aggagttgtt ggagggtatg ttgttgcccg aaatttgttg cttcaaaagc
      241 aatcacaagc tagacaaact gccgaagata ttgtaaatca agcacataaa gaagctgaca
      301 atatcaaaaa agagaaatta cttgaggcaa aagaagaaaa ccaaatccta agagaacaaa
      361 ctgaagcaga actacgagaa agacgtagcg aacttcaaag acaagaaacc cgacttcttc
      421 aaaaagaaga aaacttagag cgcaaatctg atctattaga taaaaaagat gagattttag
      481 agcaaaaaga atcaaaaatt gaagaaaaac aacaacaagt agatgcaaaa gagagtagtg
      541 ttcaaacgtt aataatgaag catgaacaag aattagaacg catctccggt ctcactcaag
      601 aagaagctat taatgagcaa cttcaaagag tagaggaaga actgtcacaa gatattgcag
      661 tacttgttaa agaaaaagaa aaagaagcta aagaaaaagt tgataaaaca gcaaaagaat
      721 tattagctac agcagtacaa agattagcag cagatcacac aagtgaatca acggtatcag
      781 tagttaactt acctaatgat gagatgaaag gtcgaatcat tggacgagaa ggacgaaaca
      841 tccgcacact tgaaacttta actggcattg atttaattat tgatgacaca ccagaagcgg
      901 ttatattatc tggttttgat ccaataagaa gagaaattgc tagaacagca cttgttaact
      961 tagtatctga tggacgtatt catccaggta gaattgaaga tatggtcgaa aaagctagaa
     1021 aagaagtaga cgatattatt agagaagcag gtgaacaagc tacatttgaa gtgaacgcac
     1081 ataatatgca tcctgactta gtaaaaattg tagggcgttt aaactatcgt acgagttacg
     1141 gtcaaaatgt acttaaacat tcaattgaag ttgcgcatct tgctagtatg ttagctgctg
     1201 agctaggcga agatgagaca ttagcgaaac gagctggact tttacatgat gttggtaaag
     1261 caattgatca tgaagtagaa ggtagtcatg ttgaaatcgg tgtagaatta gcgaaaaaat
     1321 atggtgaaaa tgaaacagtt attaatgcaa tccattctca ccatggtgat gttgaaccta
     1381 catctattat atctatcctt gttgctgctg cagatgcatt gtctgcggct cgtccaggtg
     1441 caagaaaaga aacattagag aattatattc gtcgattaga acgtttagaa acgttatcag
     1501 aaagttatga tggtgtagaa aaagcatttg cgattcaggc aggtagagaa atccgagtga
     1561 ttgtatctcc tgaagaaatt gatgatttaa aatcttatcg attggctaga gatattaaaa
     1621 atcagattga agatgaatta caatatcctg gtcatatcaa ggtgacagtt gttcgagaga
     1681 ctagagcagt agaatatgcg aaataatttt tgtctccctc acaaattagt gagggagctt
     1741 ttttaagttg tagtctcaat ctaattagac agcactttat cggtaataac tatagtaaac
     1801 agtggttatt tgaaagtaag acggacctta tattaaataa gaagttattg cttttaataa
     1861 aaatgtttta ggcttcgtat ttactatatt tatattatgt aaacctataa agatgagtgc
     1921 ttttctaacc aataaaaaag aagagaagat gtaacacatc ttctcttctg caatattaat
     1981 taggatttat ttctaagttg agttatttta attgtaaatc tgttttcttt aattctttta
     2041 taacttctgc agtatcataa caatttgttg caattgttga atatctctct gctaaacgat
     2101 atgcattaat gtaaagcttt aaactttctt tagctatatc ctctgcatct tcgaattttg
     2161 atgggttaga cataaccact aattctgcaa atttttctgg atcaatatta atagacatgt
     2221 atttatttac aactcctatt tattttgatg tcttaatact aacatattga agttttcaga
     2281 caaagtaatg tctctctata attgaagaaa aataattcaa agaatttaat atttacttta
     2341 aatattagaa cgtatgaatt aaactagtat ttatgagagg atgaacaaaa catgagaata
     2401 atgtttatag gggatatcgt aggtaaaatt ggacgagacg caattgaaac gtacatacct
     2461 caactgaagc aaaagtataa accaacagtt acaattgtaa atgctgaaaa tgcagcacat
     2521 ggtaaaggtt tgactgaaaa aatatataaa caattactaa gaaatggtgt agatttcatg
     2581 actatgggta atcacacata tggtcaacgt gaaatttatg attttataga tgaagcaaaa
     2641 cgactagtaa gaccagcgaa ttttccggat gaagcgccgg gaattggtat gagatttata
     2701 caaattaatg atattaaact tgcagttatt aatctgcaag gaagagcgtt tatgccagat
     2761 attgatgatc cttttaaaaa ggcagatcaa ttagtcaagg aagcacaaga acaaactccg
     2821 tttatatttg ttgattttca tgcagaaaca acttctgaaa agtatgcaat gggatggcat
     2881 ttagatggta gagctagcgc tgttgttgga acgcatacac acattcaaac agcagatgaa
     2941 cgtattttac caaaggggac agggtatata acggatgttg gtatgacagg tttttatgac
     3001 ggcattttag gaataaataa aacagaggta attgagcgtt ttatcactag tttgccacaa
     3061 agacatgttg ttccaaatga aggtagaagt gtattatctg gtgttgttat tgatttagac
     3121 aaagaaggta aaacaaagca catcgaacgt atattgataa atgatgacca tccattttca
     3181 acattttaaa attacgtaag taaacattcg aattggaccc tatcgtccat tagtatgaat
     3241 ttaatatagt accactgttt acatagtaaa tcggtggttc tttttgttat catttaatat
     3301 gaaatatatc cataggaggc atataactat gaaaccacaa ttatcgtgga aagttggcgg
     3361 tcaacaaggc gaaggtattg aatcaactgg ggaaatcttc gctacggcta tgaatagaaa
     3421 aggatattat ttatatggat atagacattt ttcaagtcgt atcaaaggtg gacatacgaa
     3481 taataaaatt agagtttcta cgacgcctgt tcatgcaatt agtgatgatt tagatatttt
     3541 gattgcattt gaccaagaaa caattgatgt taaccatcat gaaatgagag aagacagtat
     3601 tattttagct gatgccaagg ctaaacctgt gaaaccagaa ggatgtcatg cacagcttat
     3661 tgaattacct tttacagcaa ccgctaaaga attaggtaca gcattaatga aaaacatggt
     3721 tgcaataggt gctactagcg cattgatgaa tttgaataca aatacatttg aagaacttat
     3781 tactaatatg ttttctaaaa aaggtgacaa ggtagttgaa gtcaatatcc aagcattaaa
     3841 cgaaggttat caattaatgc aatcccgctt acctgaaatc gacggggact ttgaattaga
     3901 gtcaacagat gcactaccac atctatatat gattggtaac gatgccattg gattaggtgc
     3961 aattgctgca ggttcacaat ttatggcggc atatcctatt acacctgcgt ctgaagttat
     4021 ggaatatatg attgccaata tatctaaagt aaacggagcg gttattcaaa cagaagatga
     4081 aattgctgct gtaactatgg ctattggtgc aaattatggt ggagtcagag cgtttacggc
     4141 tagtgctggt ccaggtttat ctttaatgat ggaagcaatt ggattatctg gtatgactga
     4201 aacgccatta gtcattatta atactcaacg aggtggacct tctactggat tacctacgaa
     4261 acaagaacag tcagatttaa tgcaaatgat ttatggtaca catggtgata ttccaaaaat
     4321 agttgtagca ccaactgatg cagaagatgc attttattta actatggaag cctttaattt
     4381 agcagaacaa tatcaatgcc ctgttatagt tttaagtgat ttgcaattat ctttaggtaa
     4441 acaaactgtt gaaaaattag attataatcg cattgaaatt aaacgtggtg aaatcattca
     4501 atctgatatt gaacgtgaag aagatgataa aggttatttc aagcgttatg cgttaacatc
     4561 caatggtgtt tctcctagac ctatccccgg tgttaaagga ggtattcatc atataactgg
     4621 tgtggaacac aatgaagaag gtaaacctag tgaatctgcg tcaaatagac aacaacaaat
     4681 ggaaaaacga atgcgtaaaa ttgagcagtt actaattgaa tcgccagtag aagctaactt
     4741 acaacatgag gatgcagata ttctttatat cggttttatt tctacaaaag gtgcaattca
     4801 agaaggtagt aaccgtttga atcaacaagg cataaaagtt aacactatac aaattagaca
     4861 attgcatcca ttcccaacaa gcgttattca agatgcagtt aataaagcga agaaagtcgt
     4921 tgtagtggag cacaattatc aaggacaatt ggctagtatt ataaaaatga atgtcaatat
     4981 tcatgataag attgaaaatt atacaaagta tgatgggaca cctttcctac cacatgaaat
     5041 cgaagaaaaa ggcaaaataa ttgctactga aataaaggag atggtataga tggcgacatt
     5101 taaagatttt agaaataatg ttaagcctaa ctggtgcccc ggatgtggcg atttctcagt
     5161 acaagctgca attcaaaaag cagccgcaaa tatagggtta gaacctgaag aagtagctat
     5221 catcaccggt ataggatgtt ctggccgtct ttcaggatat attaattctt atggcgttca
     5281 ttctattcac ggacgtgcat tacctttagc tcaaggtgta aaaatggcga ataaagattt
     5341 aactgttatt gcatcgggag gagatggtga tggttatgct ataggtatgg ggcatacaat
     5401 ccatgcttta agaagaaata tgaacatgac gtatatagtc atggataatc aaatttatgg
     5461 tttgacaaag ggacaaacat cgccgtcatc agcagtagga tttgttacta aaacaacgcc
     5521 aaaaggtaat atagaaaaaa atgttgcgcc tttagaatta gcattatcat ctggtgccac
     5581 atttgtagcc caaggttttt caagcgatat taaaggatta acaaaactaa ttgaagatgc
     5641 aattaatcat gatggatttt cattcgttaa tgtcttttca ccatgtgtga cttataataa
     5701 aattaacaca tacgattggt ttaaagaaca tttaacaagt gttgatgaca ttgaaaatta
     5761 tgattctaca gataaacaat tagcgactaa aactgttatt gaacatgaat ctttagtaac
     5821 tggtattgtt tatcaagata aagaaacacc atcatatgaa tctcaaatta aagagttaga
     5881 tgatacacca cttgctaaaa gagatatcaa aattactgaa gacaccttca atgcattaac
     5941 tgaacaattt atttaataat taaaactgag tcgtataaat gtatttataa cagatccatt
     6001 tatgctactc agttttttac tattacaaaa aataaaggag tttttaaaaa tgaaagacac
     6061 attaatgagt atacaaataa ttcctaaaac accaaacaat gacaatgtta taccttacgt
     6121 agacgaggcg attaaaataa ttgacgaatc tggtttgcat tttagagtag gtccgttaga
     6181 aacgacagta caaggaaata tgaatgaatg tttaatttta atacaatcat taaatgaacg
     6241 aatggtggaa cttgaatgtc caagtattat tagccaagtt aagttttatc atgtgccaga
     6301 tggcatcact attgaaactt taactgaaaa atatgatgaa taacattaaa agtgaagtaa
     6361 actggatttg aattggcttg ttagagatga cgtataactt taactgtttt tgcactttat
     6421 agttaaattt aatata
//
LOCUS       DANBWH010000154         6361 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.160, whole genome shotgun sequence.
ACCESSION   DANBWH010000154 DANBWH010000000
VERSION     DANBWH010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6361
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.160"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            139..453
                     /locus_tag="PPY92_002075"
     CDS             139..453
                     /locus_tag="PPY92_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626060.1"
                     /translation="MYFVLAIFTIISASVSLGYSIQACASSHNINAYYALSRSLPLFL
                     LAIFSLVIHSAIFLITISIAMILVQFLDAIVGYKSEDVFKTYGPLATSVVNLILLIVF
                     LF"
     gene            complement(459..598)
                     /locus_tag="PPY92_002076"
                     /pseudo
     CDS             complement(459..598)
                     /locus_tag="PPY92_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499406.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(785..2182)
                     /gene="sufB"
                     /locus_tag="PPY92_002077"
     CDS             complement(785..2182)
                     /gene="sufB"
                     /locus_tag="PPY92_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDJ2626061.1"
                     /translation="MAKKAPDVGDYKYGFHDDDVSIFRSERGLTENIVREISNMKNEP
                     EWMLDFRLKSLKLFYKMPMPQWGGDLSELNFDDITYYVKPSEQAERSWDEVPEEIKRT
                     FDKLGIPEAEQKYLAGVSAQYESEVVYHNMEKELEEKGIIFKDTDSALQENEELFKKY
                     FASVVPAADNKFAALNSAVWSGGSFIYVPKNIKLDTPLQAYFRINSENMGQFERTLII
                     ADEGASVHYVEGCTAPVYTTSSLHSAVVEIIVHKDAHVRYTTIQNWANNVYNLVTKRT
                     FVYENGNMEWVDGNLGSKLTMKYPNCVLLGEGAKGSTLSIAFAGKGQVQDAGAKMIHK
                     APNTSSTIVSKSISKNGGKVIYRGIVHFGRKAKGARSNIECDTLILDNESTSDTIPYN
                     EVFNDQISLEHEAKVSKVSEEQLFYLMSRGISEEEATEMIVMGFIEPFTKELPMEYAV
                     EMNRLIKFEMEGSIG"
     gene            complement(2333..2797)
                     /locus_tag="PPY92_002078"
     CDS             complement(2333..2797)
                     /locus_tag="PPY92_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HDJ2626062.1"
                     /translation="MNFNNLDQLYRSVIMDHYKNPRNKGVLDNGSMTVDMNNPTCGDR
                     IRLTFDIEDGIIKDAKFEGEGCSISMASASMMTQAVKGHSLGEAMQMSQEFTKMMLGE
                     DYVITEEMGDIEALQGVSQFPARIKCATLAWKALEKGTVAKEGKAEGTTEEE"
     gene            complement(2787..4037)
                     /locus_tag="PPY92_002079"
     CDS             complement(2787..4037)
                     /locus_tag="PPY92_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDJ2626063.1"
                     /translation="MNEVAEHSFDVNEVIKDFPILDQKVNGKRLAYLDSTATSQTPMQ
                     VLNVLEDYYKRYNSNVHRGVHTLGSLATDGYENARETVRRFINAKYFEEIIFTRGTTA
                     SINLVAHSYGDANVEEGDEIVVTEMEHHANIVPWQQLAKRKNATLKFIPMTADGELNI
                     EDIKQTINDKTKIVAIAHISNVLGTINDVKTIAEIAHQHGAIISVDGAQAAPHMKLDM
                     QEMNADFYSFSGHKMLGPTGIGVLFGKRELLQKMEPIEFGGDMIDFVSKYDATWADLP
                     TKFEAGTPLIAQAIGLAEAIRYLERIGFDAIHKYEQELTIYAYEQMSAIEGIEIYGPP
                     KDRRAGVITFNLQDVHPHDVATAVDTEGVAVRAGHHCAQPLMKWLNVSSTARASFYIY
                     NTKEDIDQLINALKQTKEFFSYEF"
     gene            complement(4143..5450)
                     /gene="sufD"
                     /locus_tag="PPY92_002080"
     CDS             complement(4143..5450)
                     /gene="sufD"
                     /locus_tag="PPY92_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDJ2626064.1"
                     /translation="MTTDILNISEEQLVDYSKAHNEPSWMTELRKKALKLTETLEMPK
                     PDKTKLRKWDFDSFKQHDVKGDVYQSLSQLPESVREIIDVDHSKNLVIQHNNTIAYTQ
                     VDDNASKDGVIVEGLADALMNHSDLVQKHFMKDAVTVDEHRITALHTALVNGGVFVYV
                     PKNVVVEHPVQYVVLHDDKNASFYNHVIIVTEESAEVTYVENYLSNASGEGNQLNIIS
                     EVIAGANSNITYGSVDYMDKGFTGHIIRRGITEADASINWALGLMNEGSQIIDNTTNL
                     FGDRSTSSLKSVVVGTGEQKINLTSKIVQYGKETDGYILKHGVMKEHASSVFNGIGYI
                     KHGGTKSIANQESRVLMLSEHARGDANPILLIDEDDVQAGHAASVGRVDPDQLYYLMS
                     RGISQREAERLVIHGFLDPVVRELPIEDVKRQLREVIERKVSK"
     gene            complement(5548..6309)
                     /gene="sufC"
                     /locus_tag="PPY92_002081"
     CDS             complement(5548..6309)
                     /gene="sufC"
                     /locus_tag="PPY92_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDJ2626065.1"
                     /translation="MASTLEIKDLHVSIEDKEILKGVNLTINTDEIHAIMGPNGTGKS
                     TLSSAIMGHPSYEVTKGEVLLDGVNILELEVDERAKAGLFLAMQYPSEITGVTNADFM
                     RSAINAKREEGQEINLMQFIKKLDKNMDFLDIDKDMAQRYLNEGFSGGEKKRNEILQL
                     MMLEPKFAILDEIDSGLDIDALKVVSKGINQMRGENFGALMITHYQRLLNYITPDKVH
                     VMYAGKVVKSGGPELAKRLEEEGYEWVKEEFGSAE"
BASE COUNT         1940 a         1227 c          896 g         2298 t
ORIGIN      
        1 tcagtttatg acatagttat aatcatttga aaccattata atgataacaa ccagtttctc
       61 ataaatttat tctaattatc ttttaaatat ctttagacaa gtacttgttt atattcaaat
      121 atagaaagaa ggattaacat gtattttgtt ttagcaatat ttacaatcat tagtgccagt
      181 gtaagtttag gttattcaat tcaagcatgt gcatctagtc ataatataaa tgcatattat
      241 gcacttagtc gaagcttacc tttattttta ttagctattt tttctttagt cattcatagt
      301 gctatatttt tgataactat atccattgca atgattttag ttcaattttt agatgcgatt
      361 gttggttata aaagtgaaga tgtctttaaa acttatggtc cattagcaac atctgtagtg
      421 aacttaatat tattaatagt tttcttattt taactcactt atacaacgaa tcttaatcga
      481 actaatatcg aattatcttt aaagaatatt tgatgtagtt ttcaattaat ttaataaaga
      541 ccagcactct aatgccacaa tcatattgta tttgtgttgt cgctttatcc accatcaatg
      601 attatttttt acaccaatca aaaaatcgga ctgatataaa taagtacaaa gcttatctat
      661 caatccgatt tagttataaa acaaaaaaag ccacagtaat gtggcttttt gttatattca
      721 gtatcaaaat ggtatcaata cccatttccg gaagtcaaga atggcttaac aacgctgttt
      781 aaagctatcc aatactacct tccatttcga acttgattaa acggttcatt tcgaccgcgt
      841 attccattgg aagttctttt gtaaatggtt cgatgaatcc cataacaatc atttctgtcg
      901 cttcttcttc agaaatacca cgactcatta gataaaataa ttgttcttca gaaacttttg
      961 aaaccttggc ttcatgttct aatgatattt gatcgttgaa tacttcgtta tatggaattg
     1021 tatctgatgt tgattcgtta tctaagatta atgtatcaca ttcaatattt gaacgagcac
     1081 cttttgcttt acgtccaaaa tgaacaatac cgcgataaat aactttacca ccatttttag
     1141 aaatagattt agaaacaatc gttgaagatg tatttggcgc tttatgaatc attttagcac
     1201 cggcatcttg aacttgtcct ttaccagcaa atgcaataga taatgtacta ccttttgcac
     1261 cttcacctaa aagaacacag tttggatatt tcatcgttaa cttagaacct aagttaccat
     1321 ctacccattc catatttccg ttttcataaa caaaagtacg ttttgtaact aaattgtata
     1381 cattgttcgc ccagttttga atcgtagtat aacgaacgtg cgcatcttta tgcacaatga
     1441 tttccacaac agcagagtgt aaagaactag ttgtataaac tggtgcagta caaccttcta
     1501 cgtaatgtac agaagcacct tcatcagcaa tgattaatgt acgttcaaat tgacccatgt
     1561 tctcagagtt aatacggaaa taagcttgta gtggcgtatc tagtttgata tttttaggta
     1621 cataaatgaa cgaaccacct gaccatactg ctgagtttaa cgccgcaaat ttgttatctg
     1681 ctgcaggtac tacagaagca aagtattttt tgaataattc ttcattttct tgtaaagcac
     1741 tatctgtatc tttaaagata ataccttttt cttcaagttc tttttccata ttatggtaaa
     1801 caacttcaga ttcatattga gcagaaacac cagctaaata tttttgttca gcttcaggaa
     1861 ttcctaattt atcgaaagtt cttttaattt cttctggcac ttcatcccat gaacgttcag
     1921 cttgttctga aggctttaca tagtaagtaa tgtcatcgaa attcaattct gataagtcgc
     1981 caccccattg aggcattggc attttataaa acaattttaa tgatttaaga cggaaatcta
     2041 acatccattc cggctcattt ttcatgttag aaatttctct aacgatattc tcagttaaac
     2101 cacgttctga tctgaaaatg gatacatcat cgtcgtggaa tccatattta taatccccaa
     2161 catcaggtgc ttttttagcc atttcaatca ctccttaaaa atatatttac aacggcgtgc
     2221 gtaagttttt aattcatgat gtaaaccata ttataacaat gacatgacat cttataaaaa
     2281 tttttatact tttatatgtc taatatcaaa atcatctatg attaacagca ttctattctt
     2341 cttcagtcgt accttctgct ttaccttctt tagcaacagt acctttttcc aatgctttcc
     2401 aagctaatgt ggcacattta atacgagctg ggaattgaga tacaccttgc aatgcttcaa
     2461 tatctcccat ttcttctgta atcacatagt cttcaccaag catcattttc gtaaattctt
     2521 ggctcatttg cattgcttct ccaagtgaat gacctttaac agcttgtgtc atcatcgatg
     2581 cacttgccat tgaaatcgaa caaccttcac cttcaaactt agcatctttt ataatgccgt
     2641 cttctatatc aaatgttagt cgtatacggt caccgcatgt cgggttattc atatctactg
     2701 tcatagaccc gttatctaat acacctttat ttctaggatt tttataatga tccataatga
     2761 cagatctata taattgatct agattattaa aattcataag agaaaaactc cttcgtttgt
     2821 ttcaaggcat ttattaactg atcaatgtct tctttcgtgt tgtatatata aaaactcgct
     2881 ctagctgttg aagacacatt taaccatttc attaacggtt gcgcacaatg atgcccagct
     2941 ctaaccgcta caccttctgt atctacggct gtagcaacat cgtgtggatg tacatcttgt
     3001 aaattaaacg ttattacacc tgcacgacga tccttaggcg ggccataaat ttcaattcct
     3061 tcaattgcag acatttgctc ataagcatat atcgttaatt cttgttcata tttatgaatt
     3121 gcatcaaaac ctatgcgttc taaatagcga atagcttctg caagcccaat tgcttgagca
     3181 attaatggag tacccgcctc aaatttagta ggtaaatcag cccatgttgc atcatactta
     3241 cttacaaaat caatcatgtc accaccgaac tcaatcggtt ccattttttg tagtaactca
     3301 cgtttaccaa ataatacgcc aatacctgtt ggtccaagca ttttatgacc actaaaacta
     3361 taaaaatcag cattcatttc ttgcatatca agtttcatat gtggtgctgc ttgcgcccca
     3421 tcaacactga taattgcgcc atgttgatga gctatttctg caatggtttt aacatcatta
     3481 attgtaccga gtacattaga aatatgtgca atagcaacga tctttgtttt atcattaatc
     3541 gtttgcttaa tatcttcgat gtttaattca ccgtcagctg tcattggtat aaatttcaat
     3601 gtcgcatttt tacgctttgc taactgttgc caaggaacaa tattggcatg atgctccatt
     3661 tcagtgacaa caatttcatc gccctcttca acatttgcat caccatagct atgtgctaca
     3721 aggttaatcg acgcagttgt tccgcgtgtg aaaatgattt cttcaaaata cttcgcatta
     3781 ataaaacgac gaacggtttc acgggcattt tcataaccat cagttgccaa tgatcctaat
     3841 gtatgaacac cacgatgaac gtttgaatta taacgcttgt agtaatcctc taaaacattt
     3901 aacacttgca taggcgtttg acttgtcgct gttgaatcaa gatatgctaa acgtttgcca
     3961 ttgacttttt gatctaatat cggaaaatcc ttgattactt cattaacgtc aaatgagtgt
     4021 tcggccactt cattcacgac ctttcttaaa taaaaatcct aatcatttaa atactgacgt
     4081 tgtattagtc ttataccaat atcgacagtc tatatctatt acaaactttt attttcaaaa
     4141 tattatttag aaactttgcg ttcaattact tctctcaatt gacgtttaac gtcttcgata
     4201 ggtaattcac gtactactgg atctaagaaa ccatgtataa caagacgttc cgcttctctt
     4261 tgagaaatac cacgactcat taaatagtaa agttgatctg gatcaacacg acctactgat
     4321 gcagcatgac cagcttgtac atcatcttca tcaattaata aaataggatt cgcgtcacca
     4381 cgagcatgtt cagataacat taatacacgt gattcctgat tagcaattga tttagttcca
     4441 ccatgcttaa tgtagccgat accattaaat acagatgatg catgttcttt cataacacca
     4501 tgtttaagga tataaccatc tgtttcttta ccatattgta cgattttaga tgttagatta
     4561 attttttgtt ctcctgtacc tacaactact gatttaagtg aacttgttga acgatcacca
     4621 aataaatttg ttgtattatc aataatttgg ctaccctcat tcattaaacc tagtgcccaa
     4681 ttaattgagg catccgcttc agtaatacca cgtcgaatga tatgacctgt aaagccttta
     4741 tccatatagt ccactgagcc atatgtgata tttgaatttg caccagcaat cacttcagaa
     4801 ataatattta attgatttcc ttcaccagat gcatttgata agtaattttc aacatatgtg
     4861 acttcggcgc tttcttcagt aacgatgata acatggttat aaaagcttgc atttttgtcg
     4921 tcgtgcaaca caacgtattg tactggatgt tctacaacta catttttagg aacataaaca
     4981 aatacgccac cattaactaa tgccgtgtgt agcgctgtga tacgatgttc atctactgtt
     5041 actgcatctt tcataaagtg cttttgtact aaatcactat ggttcataag agcgtctgct
     5101 aaaccttcaa cgataacgcc atctttcgat gcattatcat caacttgtgt gtacgcaatc
     5161 gtattattat gttgaattac taagttttta gaatgatcta cgtcaataat ttctcttact
     5221 gactcaggta attgtgataa agattgataa acatcacctt ttacatcgtg ttgtttaaaa
     5281 gaatcaaaat cccattttct taattttgtt ttatcaggtt ttggcatttc taaagtttct
     5341 gttaatttca aagctttttt acgtaattct gtcatccaag aaggttcatt gtgggcttta
     5401 gaataatcaa caagttgttc ttcagaaatg ttcaaaatat cagttgtcat aactttttcc
     5461 tcctacttga tgaaatttac tatttcgtat aaatgttcat tttcatcata tatagatgaa
     5521 catctcatgg atactgtatt aataagatta ttcagctgaa ccgaactctt ctttaaccca
     5581 ttcatatcct tcttcttcaa gacgttttgc taattctgga ccaccagatt taacgacttt
     5641 accagcatac attacatgta ctttatcagg agtaatgtaa tttaataatc gttgatagtg
     5701 tgtaatcatt aatgcaccaa agttttcccc acgcatttgg ttaatacctt tagatacaac
     5761 ttttaatgca tcgatgtcta accctgaatc gatttcatct aagattgcaa acttaggttc
     5821 taacatcatt aattgtaaga tttcgttacg tttcttctct ccacctgaga aaccttcatt
     5881 taaataacgt tgtgccatgt ctttatctat gtctagaaaa tccatgtttt tatctaattt
     5941 cttaataaat tgcattaagt tgatttcttg tccttcttca cgtttcgcat tgattgctga
     6001 acgcatgaaa tcagcatttg taacacctgt aatttctgat ggatattgca ttgccaagaa
     6061 taatcctgct tttgctcttt catcaacttc taattctaaa atatttacac cgtctaaaag
     6121 tacttctcct ttagttactt catagcttgg gtgtcccata attgcagatg ataaagttga
     6181 tttacctgtc ccgtttggtc ccataatcgc atgtatttca tcagtgttaa ttgtcaagtt
     6241 aacacctttt aagatttctt tatcctcaat agacacatgt aggtctttga tttctaatgt
     6301 tgatgccatt ttatattccc tccgtaaaat ataaagtttt cttaactagt ttataataat
     6361 t
//
LOCUS       DANBWH010000155         6352 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.161, whole genome shotgun sequence.
ACCESSION   DANBWH010000155 DANBWH010000000
VERSION     DANBWH010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6352
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.161"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(332..1795)
                     /locus_tag="PPY92_002082"
     CDS             complement(332..1795)
                     /locus_tag="PPY92_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HDJ2626066.1"
                     /translation="MGEFKGFMKYDKQYLGELSLVDRLKHHKAYQQRFTKEDASIQGA
                     RCMDCGTPFCQTGQQYGRETIGCPIGNYIPEWNDLVYHQDFKTAYERLSETNNFPDFT
                     GRVCPAPCESACVMKINRESIAIKGIELTIIDEAFENGWVAPKVPSRRRDEKVAIVGS
                     GPAGLTAAEELNLLGYQVTIYERARESGGLLMYGIPNMKLDKDVVRRRIKLMEEAGIT
                     FINGVEVGVDIDKATLESEYDAIILCTGAQKGRDLPLEGRMGDGIHFAMDYLTEQTQL
                     LNGEIDDITITAKDKNVIIIGAGDTGADCVATALRENCKSIVQFNKYTKLPEAITFTE
                     NASWPLAMPVFKMDYAHQEYEAKFGKEPRAYGVQTMRYDVDDKGHIRGLYTQILEQGE
                     NGMVMKEGPERFWPADLVLLSIGFEGTEPTVPNAFNIKTDRNRIVADDTNYQTNNEKV
                     FAAGDARRGQSLVVWAIKEGRGVAKAVDQYLASKVCV"
     gene            complement(1813..6312)
                     /gene="gltB"
                     /locus_tag="PPY92_002083"
                     /pseudo
     CDS             complement(1813..6312)
                     /gene="gltB"
                     /locus_tag="PPY92_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499018.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
BASE COUNT         1840 a         1458 c          936 g         2118 t
ORIGIN      
        1 gagccgttaa gcccctgtcg gttttcaaga ccgatccctt cagccggact tgggtattcc
       61 tcctaaacac aatatttatc atatatgaac gttctgtaaa agtcaatatt gtttttaagc
      121 attacatgat taccacaaat cattgtcttc cactacagtt gacctataaa cgaatgtaaa
      181 aaatcactac ttataattaa tcgatttgtt gtcttacaaa cctttgtttt taggttacaa
      241 agatggcatg tgacgttgat agatactaga ttatttcaaa ctcgattcag catgacacaa
      301 cgtcaacgta accaccattt ccatacaaag attatacaca aactttactc gctaaatact
      361 gatctactgc tttcgctacg cctctacctt ctttaattgc ccatacaact aaactttgac
      421 cacgtctagc atctccagca gcaaatacct tttcattatt agtttgatag tttgtatcat
      481 ccgccacgat tcgatttcta tccgttttaa tgttaaaagc attcggtact gttggttctg
      541 taccttcgaa gccgattgat aataatacaa ggtcagcagg ccaaaatctt tcaggtcctt
      601 ctttcatgac cataccattt tcgccttgct ctaaaatttg agtatacaaa ccacgtatgt
      661 gtcctttatc gtcaacatcg taacgcattg tttgaacacc atatgcacgt ggttccttac
      721 caaacttagc ttcgtactct tggtgcgcat agtccatttt aaacaccggc attgctaaag
      781 gccatgatgc attttctgta aatgtaattg cttctggcaa tttcgtatat ttattaaatt
      841 gaacaatcga tttacaattt tctcttaatg ctgtcgctac acagtctgcc cctgtatcac
      901 cagcaccaat gataatgaca ttcttatctt ttgcagttat tgtaatatca tcaatttctc
      961 catttaacag ctgcgtttgt tcagttaaat aatccatagc gaaatgtata ccatcaccca
     1021 tgcgtccttc taaaggtaaa tctctacctt tttgtgcacc agtacataat ataatggcat
     1081 catactcaga ttctaacgtt gctttatcaa tatcaacgcc tacttcaaca ccattaatga
     1141 aagtaatgcc cgcttcttcc attaacttaa tacgacgtcg aaccacatct ttatcaagct
     1201 tcatatttgg aataccatac attaataaac cgcctgattc tctagcacgt tcataaatag
     1261 tcacttgata tcccagtaga ttaagttctt cagcagcagt taatcctgct ggaccgcttc
     1321 caacgattgc cactttttca tctctacggc gactcggaac tttcggcgct acccaaccat
     1381 tttcaaaagc ttcatcaata attgtgagtt caataccttt aatcgcaatc gattctctat
     1441 taatcttcat cacacaagca ctttcgcatg gtgcaggaca tacacgccct gtaaagtcag
     1501 gaaagttatt tgtttcgctt aagcgttcat aagcagtttt aaaatcttga tgatacacta
     1561 agtcgttcca ttcaggaatg tagtttccaa ttggacaacc tattgtttcc ctaccatact
     1621 gttgtccggt ttgacaaaac ggcgttccac aatccataca tcgcgcacct tggatagagg
     1681 catcttcttt agtaaatcgt tgttgatatg ctttatgatg cttcaaacgg tctaccagtg
     1741 ataattcacc taagtactgt ttgtcatact tcataaatcc tttaaattca cccatcgtat
     1801 ctcccccttt ccttaataca caacggctgg tttatgttta gcgtcgattg ttttactgtc
     1861 atcgtaaaat gcagctaaca tcgcttcatc ttcattgtca tgtaatgatt tgtgcaaatg
     1921 aattttttgc atcattaatt gataatcttt aggaataact ttaacgacga catcttcaat
     1981 gcgatcaaaa tgttttaaca catgaatcgc tctcgtacta ttcgtgtgtg acacatgttc
     2041 ttccagcatt tgcttaatga atgctttttc ttcttggtgt ttaatctttg taaacgaaag
     2101 cgtatctagt tgattatttt caacaaaagc ttctacatca gacgggataa cgtaagcaat
     2161 accaccactc ataccttgac cgaagttctt acctacatca cctaaattaa tgacatgtcc
     2221 accagtcatg tactctaatc catggtcacc gataccttca acgacaacat ctacaccact
     2281 atttctaata cagaatcttt ctcctgcact accgttaata aatgccttac cacctgtcgc
     2341 accatagaat gagacgttac cagcaataat ttcattttgt cgttcttcaa aaggtgcttt
     2401 gacaatgacc gtaccaccag ataatccttt accaacatag tcattcgcat ctccagtatg
     2461 atgaatcatt aagcctttcg gtgcatatgc tgcaagactt tgaccagcat gaccattcgt
     2521 ataaacatta attgtatttt caggaagtcc tgcttctcca tattgtttcg aaatctcact
     2581 acctgtaata actcctacat cacgttgttc attatttact gtaaagctac ctgtatagcg
     2641 acgtccttca gcaatatatg gcttcgttac ttcatataaa tttgttaaat caaatccatg
     2701 ctcaagatta tgattttgtt gaatttcttt tgtgtttggc ccatcgaaag gacataacag
     2761 tttttcaaca tcaatactag ccactttgct attcgctttt aatgttgatg atcgttgtaa
     2821 taaatcagtt cttccaacta agtcttctac acgtttcaaa cctaaagatg ctaaaatttc
     2881 tcttaattct tgtgcaataa aatgcataaa attaacaaca tgatgtgctt tacctctata
     2941 taaagcacgt aaatctttgt tttgagttgc aactcctact ggacatgtat ctttatggca
     3001 tacacgcatc ataatacagc ccaacaccac taatggtgca gttgcaaatc caaattcttc
     3061 cgctccaagc gcacatgcgt acgctacatc tttaccagtt aataacttac cgtctgtttc
     3121 taacttaaca cgacttctta agtcatttag ttttaatgtt tgatgtgttt ctgctaaacc
     3181 aatctcccaa ggaacaccgg catgctgaat actcgtttta ggtgaagccc ctgtaccacc
     3241 atcgtaacca ctgatgacaa ttttatctgc aaatgctttt gccaccccag atgcaatggt
     3301 accaacacct gttttcgaaa ctaattttac cgcgatatct gcatctttat tcgcattttt
     3361 caaatcatgt atcagttgcg ctaaatcttc tattgaataa atatcatgat gtggcggtgg
     3421 tgaaatcaga ccgatacctg gcgttgaccc tcttgtcttt gcaatccacg gatatacctt
     3481 agtaccaggt aattgaccac cttcaccagg ctttgcacct tgcgcaactt taatttgaat
     3541 ttctttggca tgttgtaaat aatcactagt tacaccaaaa cgcccagaag caacttgttt
     3601 aatcgcactt actttgttgc ttccatcaac ttgtacttca taacgttttg catcttcgcc
     3661 accttcacca ctattactct ttccacctaa ttggttcatg gcttgtgcta acgtttcatg
     3721 tgcttccgct gaaatcgatc cataactcat cgcccctgta ttaaagcgtt tgacaatgtc
     3781 acttaccggt tcaacttggt cgatgtcaat cggtgtacat gctttaaatt caagtaaatg
     3841 tctaatgtga tctgttctat ttttgttcac cgcttcagag tatgctttaa attgcgcata
     3901 gtcattttct ttacatgcgt gctgcaataa gaaaatagat tccggattaa aagcatgatg
     3961 ttgaccttgt tatctccatt gaaatgtact acctgatgca agataattat catcactttg
     4021 ttgacgtgct ttattttcag catcaatttg atcaatcgaa atacccgata acttagactg
     4081 tgtcccagta aaataacgat caatcacatc atgagacaag ccaatcgctt caaatatttg
     4141 tgcaccttga taactttgca ctgtcgaaat tcccatctta gccattactt taatgacacc
     4201 ttctgacaat acatccgtat atgtcttaac attatcgaca acggtgcctt gtaacccttc
     4261 tgtcaatgtc agttgttcaa ctgtacgttg cgctaggtat ggcacaattg cattcgcgcc
     4321 atatgcgagt aaacaagcaa catgatgcac ttctcgtgtc tcaccagatt tagcgactaa
     4381 acttgtagac atacgtaaat ctgctttaat aagtaattga tgcacatgac ttattgcgag
     4441 taacatcggc attgcaaagc cattgctatc aactaatcca ctgtcatcta acactagaat
     4501 ttgagcgcct tgctttacag cattcactgc ttctcggcct aatgcttcta acgcatcttc
     4561 caaatcccct tcatataccg ttgataaata agttaattta aaatgttcct gatcaatcgc
     4621 tgctaagtgt gattcattca ataccggcct tttcaattga atacgatcta aaaccgtttc
     4681 gtcaggtgct agtaagttac cttcgccacc taaataagaa agttcactcg ttacgatttt
     4741 ttcacgatac gcatcaattg gtggattcgt aacttgtgca aacagctgtt taaagtaatt
     4801 aaatagtgat tctggtcgct cgttcaacac tgcaattggc gcatcatatc ccattgcacc
     4861 gataggatcc ttcttacctt ctacaagttc ctgaatatac ttatgaatct cttctttcgt
     4921 gtatgcaaac tgacgttgta atttaaataa cgtctcatct ttccattgcg aatcttgata
     4981 ttgtatattt tcaaaatcaa agtcaacttt atggttatca atccacgctt tatatggtaa
     5041 ttctccagca atcgcacctt ttaaatcatt attttcaatg actttattct gtttaaaatc
     5101 aacaagcaat aactttccag gattcaattg acctttaaaa gcaacattac tttcaggtac
     5161 gtccacaaca cccacttcag atgaaaagac aataaagtta tctttagtaa tcgtataacg
     5221 acctggacgt aatccatttc tatctgtaag cgcgccaagt ttgtcaccgt tacagaacga
     5281 aatcattgta ggaccatccc acggttccat taaataacta taaaattcat aaaacgcacg
     5341 tacatttgca tcattcgctt cattgtataa ccaaggttca ggtatgagta gcatcgctgc
     5401 cttttctggc tccatggcta acgataagaa ctctagcgca ttatctacaa tagcagagtc
     5461 actaccatcc tcatcgacaa tttgaaacac tttatgttga tcctcgccaa ataatgtttc
     5521 gattaattta tgttggcgtg ctcgcatcca gtttacatta cctttaatcg tgttaatctc
     5581 accattatgc attaacatac ggttaggatg tgcccttttc caactcggga atgtattcgt
     5641 actaaatctc gaatgcacta accctagctt tgattgatat aaatcatccg ataaatctgt
     5701 atatagtttt ttaatttggt ctgatcgtaa ccaaccttta tatacaattg ttttgcgtga
     5761 taagctcgta aaatacaatt ctaaatcgca ctgagtcgaa tagaactcta attgttttct
     5821 cgctaaaaac aaacgctttt caacatcttc aatgccccta atatcaataa acacttgttg
     5881 aatgactggc atcgtatctg ctacatgttt agcaatggca tctttattaa ctggtacatt
     5941 acgataacca agaattgata acccttcgcc ttcaaaatat tttttaaaaa ctgcttcatg
     6001 ttcagaacct aaaatgcgtt ctttggaaaa aaataacccc acggcatatt caccttcacc
     6061 tgggatatca aagtccgtta catgttgttt gaaaaatgca aaaggtattt cagtcataat
     6121 acctgcgcca tcaccagtga tgccatctgc gccgaccccg cccctgtgat ctaagcgtcg
     6181 caacatttca agcgatttat caatgatgtc gtgagacctt ttattatcca tattcgcata
     6241 aaaaccaata ccacacgcat catgttcctc acgatagtca tataagcctt taattaattt
     6301 ctcattgtgc atgatgcaca cccctttttt agaatattct gatattagtt at
//
LOCUS       DANBWH010000156         6214 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.162, whole genome shotgun sequence.
ACCESSION   DANBWH010000156 DANBWH010000000
VERSION     DANBWH010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6214
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.162"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            250..1542
                     /gene="obgE"
                     /locus_tag="PPY92_002084"
     CDS             250..1542
                     /gene="obgE"
                     /locus_tag="PPY92_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDJ2626067.1"
                     /translation="MFVDQVKISLKAGDGGNGITAYRREKYVPFGGPAGGDGGKGASV
                     VFEVDEGLRTLLDFRYQRHFKASKGENGQSSNMHGKNAEDLVLKVPPGTIIKNVETDE
                     VLADLVEDGQRAVVAKGGRGGRGNSRFATPRNPAPDFSEKGEPGEELDVSLELKLLAD
                     VGLVGFPSVGKSTLLSIVSKAKPKIGAYHFTTIKPNLGVVSTPDQRSFVMADLPGLIE
                     GASDGVGLGHQFLRHVERTKVIVHMIDMSGSEGREPIEDYKVINQELAAYEQRLEDRP
                     QIVVANKMDLPESQDNLNLFKEEIGEDVPVIPVSTITRDNIDQLLYAIADKLEEYKDV
                     DFTVEEEESVGINRVLYKHTPSQDKFTISRDDDGAYVVSGNAIERMFKMTDFNSDPAV
                     RRFARQMRSMGIDDALRERGCKNGDIVRILGGEFEFVE"
     gene            1555..2010
                     /locus_tag="PPY92_002085"
     CDS             1555..2010
                     /locus_tag="PPY92_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDJ2626068.1"
                     /translation="MDNKDYKKFYLIREDVLPESVVKTLKIKDALKSDPTLSIYDAVK
                     QFDLSRSAFYKYRETIFPVDDKMLDHREFTLILYVTDIVGMLARVLDVISKLELSVLT
                     IHQSIPMEEKATITLSLNAKSKETSVEDVIGALRNLDYVSKVELISMSM"
     gene            2024..2626
                     /gene="ruvA"
                     /locus_tag="PPY92_002086"
     CDS             2024..2626
                     /gene="ruvA"
                     /locus_tag="PPY92_002086"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDJ2626069.1"
                     /translation="MYAYVKGKLTHLYPTHVVVETAGVGYEIQTPNSYRFQKHLDHEV
                     LIRTSLIVREDAQLLYGFSSEEEKDMFLSLIKVTGIGPKSALAILATSTPNEVKRAIE
                     NENDTYLTKFPGIGKKTARQIVLDLKGKVKITEEDSDSLLQVDATSTVQDQFVQEAML
                     ALEALGYSKRELAKVEKTLNKNKYDSVDEAVKAGLQLVVS"
     gene            2658..3662
                     /gene="ruvB"
                     /locus_tag="PPY92_002087"
     CDS             2658..3662
                     /gene="ruvB"
                     /locus_tag="PPY92_002087"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDJ2626070.1"
                     /translation="MNERMVDQSMHSEETDFELSLRPTRLRQYIGQNSIKSNLEVFIK
                     AAKLRHEPLDHVLLFGPPGLGKTTLSNIIANEMEVNIRTVSGPSLERPGDLAAILSGL
                     QPGDVLFIDEIHRLSSVVEEVLYPAMEDFFLDIIIGKGDEARSIRIDLPPFTLVGATT
                     RAGSLTGPLRDRFGVHLRLEYYNESDLKEIIIRTAEVLGTGIDEESAIELAKRSRGTP
                     RVANRLLKRVRDFQQVNEDEQIYIETTKHALGLLQVDQHGLDYIDHKMMNCIIKQYNG
                     GPVGLDTIAVTIGEERITIEDVYEPFLIQKGFLERTPRGRKATPLAYEHFAKSNEERG
                     "
     gene            3664..4689
                     /gene="queA"
                     /locus_tag="PPY92_002088"
     CDS             3664..4689
                     /gene="queA"
                     /locus_tag="PPY92_002088"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDJ2626071.1"
                     /translation="MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFK
                     DIIEYFRPGDTLVLNDTRVMPARLFGLKEETGAKVEMLMLTQIEGNDWEVLLKPAKRI
                     KVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDD
                     PDRYQTVYAKESGSAAAPTAGLHFTDELLTEIKNKGVNIAFVTLHVGLGTFRPVSVDD
                     VNDHEMHSEYYQMTQETADLLNDTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGW
                     TNIFIYPGFDFKAIDGQITNFHLPKSTLVMLVSAFSTRENVLNAYKTAVNLEYRFFSF
                     GDAMLII"
     gene            4712..5851
                     /gene="tgt"
                     /locus_tag="PPY92_002089"
     CDS             4712..5851
                     /gene="tgt"
                     /locus_tag="PPY92_002089"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016425271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDJ2626072.1"
                     /translation="MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVK
                     TMSPEELRQIEAKIILGNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFS
                     LSNLRKITEEGVEFRHHTNGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYV
                     KKSIERTTRWAKRCLDAHQRPEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGG
                     LSVGEPKPVMYKMVEHTEQFMPKDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIA
                     RNGTCMTSQGRLVIKNAKFADDLRPLDENCDCYTCQNYSRAYIRHLIKAEETFGIRLT
                     TIHNLHFLLKLMEDIRQAIREDRLLDFKEEFFEQYGLNVENPKNF"
     gene            5870..6130
                     /gene="yajC"
                     /locus_tag="PPY92_002090"
     CDS             5870..6130
                     /gene="yajC"
                     /locus_tag="PPY92_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDJ2626073.1"
                     /translation="MQFSLLIYIVVIFAVMYFLMIRPQQKRAKQHRELINNIQSGQRI
                     TTIGGIKGTVKAVDETTVVITVNGHGTELTFEKPAIKQVDPS"
BASE COUNT         2186 a          859 c         1290 g         1879 t
ORIGIN      
        1 gacgttatac taaatgtaca actgcataag agcccctaat cgctaaacta taaggggaca
       61 aaagaataca gttgcagtat aaaaagcaaa aaaagtatca tatttggtat tcggaaatat
      121 cagcaatttg agtaaagtaa atttaaaagc aactgcataa gagcccctaa ttatttacaa
      181 tatataaggg gctctaatat gctataatta ttgggaaaat gaaaattata tgtaaaagag
      241 gtgagatata tgtttgtcga tcaagtcaaa atatctctta aagccggtga tggtggtaat
      301 ggtattaccg catatagaag agaaaaatat gtaccatttg gtggaccagc tggcggtgac
      361 ggtggtaaag gtgcttcagt cgtatttgaa gtggatgaag gtttaagaac gttattagat
      421 tttagatatc aacgtcattt taaagcaagc aaaggtgaaa atggccaaag tagtaatatg
      481 catggtaaaa atgcggaaga tttagtatta aaagttccac ctggtacaat tattaaaaat
      541 gttgaaacag acgaagtgtt agcagatctt gttgaagatg gtcaaagagc tgtagtagcg
      601 aagggcggtc gaggtggccg aggtaattca cgttttgcaa cacctagaaa ccctgcacct
      661 gacttcagtg aaaaaggtga accaggtgag gaattagatg tatctttaga attgaaatta
      721 ttagctgatg taggattagt aggtttccct agtgtgggta aatcgacttt attatctatc
      781 gtttcaaaag ctaagcctaa aattggggca tatcatttta caacgattaa accaaatcta
      841 ggtgttgttt caacgcctga tcaacgtagt tttgttatgg cagatttacc aggtttaatt
      901 gaaggtgcat ctgatggcgt tggattagga catcaatttt taagacatgt agagagaaca
      961 aaagttattg ttcacatgat tgatatgagc ggttctgaag gtagagaacc tattgaagat
     1021 tataaagtca ttaatcaaga attagctgcg tacgagcaac gtttagaaga tagacctcaa
     1081 atcgtagtag ctaacaagat ggatttacct gaatcacaag ataatttaaa cttgtttaaa
     1141 gaagaaattg gcgaagatgt gccagttatt ccagtttcaa caataacgcg tgataatatt
     1201 gatcaattat tatatgcaat agcagataaa ttagaagaat ataaagatgt tgacttcaca
     1261 gttgaagaag aggagtcagt tggcattaac cgagtattat ataaacatac accgtcacaa
     1321 gataaattta caatttcaag agatgatgat ggtgcttatg tggtaagtgg taatgctatt
     1381 gaaagaatgt ttaaaatgac tgactttaac agtgatccag cagtacgtcg atttgctcgt
     1441 caaatgcgtt cgatgggtat tgatgatgca cttagagaac gtggttgtaa aaatggtgat
     1501 atcgttagaa ttcttggcgg agaatttgaa ttcgttgaat aggagcgtaa catgatggac
     1561 aataaagatt ataaaaagtt ttatttaatt agagaagatg tcttgcctga atccgtggtt
     1621 aaaacattga agattaaaga tgccttaaaa agtgatccga cattgtccat ttatgatgcc
     1681 gttaaacagt ttgatctatc tagaagtgct ttttataaat atagagaaac gatatttcca
     1741 gtagacgata aaatgcttga ccatcgagaa tttacattaa ttttatatgt aactgatatt
     1801 gtgggtatgt tggcacgtgt actagatgtt atatcaaagt tagaactatc tgtattaacg
     1861 attcatcaaa gtattccaat ggaagaaaaa gcaacaataa cattatcact gaatgctaaa
     1921 tctaaagaaa cttcagtaga agatgttatt ggtgctttga gaaatttaga ttatgtatca
     1981 aaagtagaat taattagtat gagtatgtaa ggatgtgcct ataatgtacg cgtatgtcaa
     2041 aggtaagtta acacatttat atcctacaca cgtagttgtt gaaactgctg gtgttggtta
     2101 tgaaattcaa acaccaaatt cttatcgttt tcaaaagcat ctagatcatg aagttttaat
     2161 tcgtacatct ttaattgttc gtgaagatgc acaattattg tatggattta gtagtgaaga
     2221 agagaaagat atgttcttga gtttaattaa agttactggt attggtccga aatcagcttt
     2281 agctatttta gcgacaagta cgcctaatga agtaaaacgt gccattgaaa atgaaaatga
     2341 tacgtattta actaaattcc caggaattgg taagaaaacg gcaagacaga ttgtcttaga
     2401 tttaaaaggt aaagtgaaaa tcactgaaga agatagcgat tcattattac aagtagacgc
     2461 tacttcgacg gtgcaagatc aattcgtgca agaagcaatg ttagcgttag aagcattagg
     2521 ttattctaaa cgagagcttg caaaagttga gaaaacgtta aataaaaata aatatgactc
     2581 agttgatgaa gctgttaagg caggtcttca attagttgta tcttaatttt aaatagatta
     2641 ataggggaag tgttgtcatg aatgagcgta tggttgatca atcaatgcat agtgaagaaa
     2701 ctgatttcga attgtcgctt agacctacga gattacgaca atatattggt caaaattcaa
     2761 taaaaagtaa tttagaagta tttattaaag cggctaaact tcgtcatgaa ccattagatc
     2821 atgtattgct ttttggcccc cctggattag gtaagacaac attatctaat atcattgcca
     2881 atgaaatgga agttaatata cgtacagtat cagggccttc attagaaaga cctggtgatt
     2941 tggctgcaat tttatcagga cttcaacctg gagatgtatt gtttattgat gaaatacaca
     3001 gactgagtag tgttgttgaa gaagtgttat accctgcaat ggaagatttc tttttagata
     3061 ttatcattgg taaaggcgat gaggctagaa gtatccgtat cgacttacct ccattcactt
     3121 tggtaggtgc aacaacgcga gctggcagct taacaggtcc actaagggat cgatttggtg
     3181 tgcacttaag attagaatat tataatgaat cagatttaaa agaaatcatt attagaacag
     3241 ctgaggtttt aggcacaggt attgatgaag aaagtgccat tgaacttgct aaacgttcta
     3301 gagggactcc aagagtagca aatcgactat tgaagcgggt aagagacttc cagcaagtga
     3361 atgaagatga acaaatatac attgaaacaa cgaagcacgc attaggttta cttcaagttg
     3421 atcaacacgg actagattac attgatcata aaatgatgaa ctgtattatt aagcagtata
     3481 atggtggacc tgttggttta gatacgattg ccgtaacaat tggtgaagaa cgtattacaa
     3541 ttgaggacgt ttatgagcca tttcttattc agaaaggctt tttagaacgt acgccacgtg
     3601 gcagaaaagc aacaccatta gcttatgaac attttgcaaa gtcgaatgag gagagaggat
     3661 aacgtgaata ttgaagaatt tgactatgac ttaccagaat cattaattgc tcaaacgcct
     3721 ttaaaagatc gtgatcatag tcgtttatta gtcatggata gagaaactgg tgaaatgaaa
     3781 catttacatt tcaaagatat cattgagtat tttagacctg gtgatacatt agtgcttaac
     3841 gatacgcgag taatgccagc tagacttttt ggtttaaaag aagaaactgg tgcaaaagtt
     3901 gaaatgttaa tgttaactca aattgaaggt aatgattggg aagtcttact gaaaccagct
     3961 aagcgtatta aagttggtaa taaattgaat tttggtaatg gcaaaattat agctgagtgc
     4021 ataaaagaaa tggatcaagg tggacgcatc atgcgtttac attatgaagg tattttacaa
     4081 gaaagattag atgaattagg ggaaatgcca ctgccaccat acatcaaaga acgtttagat
     4141 gatccagatc gttatcaaac agtttacgct aaagaaagtg gttcagcggc agcaccaaca
     4201 gcaggattac attttactga tgagttatta actgaaatta aaaataaagg cgttaatatc
     4261 gcatttgtta cattacatgt tgggttaggt acgtttagac cggtgagcgt cgatgatgtg
     4321 aatgaccacg aaatgcatag tgaatattat caaatgacac aagaaacagc tgatttatta
     4381 aatgatacta agtccaaagg acatcgcatt atatcagttg gtacaacttc aacacgtaca
     4441 cttgaaacaa ttcgacgcga tcatgataaa tttgttgaaa cgagtggctg gactaatata
     4501 tttatttatc caggatttga ttttaaagca attgatggcc agattactaa ttttcattta
     4561 ccaaaatcaa cattagttat gctagtatca gcgtttagta ctcgtgaaaa tgttctgaat
     4621 gcttataaaa cagcagtaaa tttagaatat agattcttta gttttggcga tgcaatgtta
     4681 attatataaa aagaatgtga ggattttgaa tatgcctgca gtaacatacg aacacattaa
     4741 aacttgtaaa caatcaggtg cgcgtttagg tatcgtgcac acaccacacg gttcatttga
     4801 aacacctatg tttatgccag ttggtactaa agcaaccgtt aaaacaatga gtccagaaga
     4861 gttaagacaa attgaagcaa aaatcatttt gggcaacaca tatcatttgt ggttacaacc
     4921 cggaaatgat attatcaaac acgctggggg attacataaa ttcatgaatt gggatggtcc
     4981 gattcttaca gattcaggcg gtttccaagt gtttagttta agtaatttac gtaaaattac
     5041 agaagaaggc gtggaattta gacatcatac taatgggtct aaattatttt tgagtcctga
     5101 gaaatcaatg caaattcaaa atgatttagg atctgatatt atgatggcat ttgatgaatg
     5161 tccaccgatg cctgctgaat atgattatgt aaaaaaatct attgaacgta caacacgttg
     5221 ggcgaaaaga tgtctagatg cacaccaaag acctgaagat caagcattgt tcggcattat
     5281 acaaggtggc gaatatgaag atttaagaga acaaagtgca aaggatttag tagaattaga
     5341 ttttcctggt tatgcaatcg gcggtttgtc agttggtgaa cctaaaccgg ttatgtataa
     5401 aatggttgaa catacagagc agtttatgcc taaagataaa ccaagatatt taatgggtgt
     5461 aggatctcca gatgcgttaa tcgaatgtag tattcgcggc atggatatgt ttgattgtgt
     5521 cttaccgaca cgtattgcca gaaatggtac ttgtatgaca tcgcaaggtc gtttagttat
     5581 taaaaatgca aaatttgcag atgatttaag accgttagat gagaattgtg actgttatac
     5641 atgtcaaaac tattcaagag cgtatatacg tcatttaatc aaggcagagg aaacttttgg
     5701 tattcgtctt actactattc ataatttaca ttttctgcta aaattaatgg aagatataag
     5761 acaagccatt cgagaagatc gtcttttaga tttcaaagaa gaattcttcg agcaatatgg
     5821 attaaatgtt gagaacccaa aaaactttta agcaagagga gcgtataaaa tgcaattttc
     5881 attactaata tatatagtcg taatttttgc ggttatgtat ttcttgatga tcagaccaca
     5941 acaaaaacgt gcgaaacagc atcgtgagtt gattaataac attcaatctg gtcaaagaat
     6001 tacaactatt ggtggtatta aaggtactgt taaagcagta gatgaaacaa ctgttgttat
     6061 tacagttaat ggtcatggta ctgaattaac tttcgaaaaa cctgctatta aacaagttga
     6121 cccttcataa cgagaagtgg tattaaaaag gatgaaacga atagcgtttc atccttttta
     6181 agttataagt agtatgtaga atagcttatt ttta
//
LOCUS       DANBWH010000157         6142 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.163, whole genome shotgun sequence.
ACCESSION   DANBWH010000157 DANBWH010000000
VERSION     DANBWH010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6142
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.163"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(30..203)
                     /locus_tag="PPY92_002091"
     CDS             complement(30..203)
                     /locus_tag="PPY92_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626074.1"
                     /translation="MWNFIKCVFKFVFSLVAITTLVAGVGVVAFAYIFKKDFEDIERK
                     TKEIISDIESKNN"
     gene            654..1694
                     /locus_tag="PPY92_002092"
     CDS             654..1694
                     /locus_tag="PPY92_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDJ2626075.1"
                     /translation="MQQVISDIMTFRGSHFELGVKTGKWLQQTPLLKNREKEWKKRVP
                     RFDIDVNETYQIFQTYAPQIWEELMGLQSILKMPTRQIILNFGHYRFTDLKESGCTVF
                     QGKDFMVRNYDYHPATYDGRYLLYQPTDSGLAQIGPVSRVTGRMDGMNESGLTMGYNF
                     MHRKKPANGFVCYMIGRLILENCRNVTEAIQLLKEIPHRSSFSYILMDKSLNHAIVEV
                     TPRSIDVRYDNICTNHFEILTHENRNYTKESKERLARTISQTNDNLDMTTAFKLFNNP
                     QYEIYSKLFKSWSGTIHTSMYQPETLTAYFTLGENAPPEIIDFKSWLDGQDLNITHFT
                     GNIDTDLTFANK"
     gene            complement(1751..2593)
                     /locus_tag="PPY92_002093"
     CDS             complement(1751..2593)
                     /locus_tag="PPY92_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDJ2626076.1"
                     /translation="MKKFFFIGLLVFVVFFTAATIIWFSYDKNKYGTKQYDKTFKDDA
                     FDNVSINLDSTELRIKRGNQFRVKYDGDNDILINIVDKTLKISDKRSKTRGYAIDMNP
                     FHENKKTLTIEMPDKMIKRLNLSSGAGSVRISDVDLENTSIQSINGEVVIKNSNLDAL
                     DSKTNNSSTYISKSNIKNSNIKVVIGTLQIDKSQIKQSIFLNDHGDIEFKNMPSKVDA
                     KASTKQGDIRFKYDSKPEDTILKLNPGTGDSVVKNKTFTNGKVGKSDNVLEFYTIDGN
                     IKVE"
     gene            complement(2590..3147)
                     /locus_tag="PPY92_002094"
     CDS             complement(2590..3147)
                     /locus_tag="PPY92_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001669025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDJ2626077.1"
                     /translation="MNKITYLNELETALDALPRHLRDQKMYEYERYFYEQELNGVDEG
                     EILKKLKDPQDVAAETKARSVIDYAESKPTFENISRAVAASLSLGILSIFVILIPVSI
                     VGLFVLALFLISLLLLFCPIILLASAISRGIVDSISNVFFAISYSGLGLVFIIVIFKI
                     LEYIYRLILKYLLWYIKTVKGSVRK"
     gene            complement(3207..3731)
                     /locus_tag="PPY92_002095"
     CDS             complement(3207..3731)
                     /locus_tag="PPY92_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626078.1"
                     /translation="MLYLNKYKERVTSVRIQNRWVVFILFLICSFGVLIGLYQYRHTK
                     TVDLSNLEINDIKLNEKFDKKGYEVNKKIKFDRFKFYNSKAHPDLTVKVREKDNIVKG
                     IILVRDEKIHTNFDGGIGSPINNAIENLGFGYKRTKVGNDFSSVKYIDRDNHLKLNLL
                     YQDLEIKRIEFFSK"
     gene            3852..4415
                     /locus_tag="PPY92_002096"
     CDS             3852..4415
                     /locus_tag="PPY92_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDJ2626079.1"
                     /translation="MTNLETYFKNSQPLNEYIDGMKTNQDTVLSIYQSFELPEDDSRI
                     KKIKEMNYSKVLVITEDWCGDAMMNLPILKHISEALNLEVRVFHRDDDTKLIDQYLTN
                     GKSRAIPIFVFLNDQFEQETVWGPRANEVQKFVTDVRADKLPSKDHPDYADLEKETHL
                     VISNRYKTDASFWKAVYNSILNKLENK"
     gene            complement(4481..5221)
                     /gene="pmtD"
                     /locus_tag="PPY92_002097"
     CDS             complement(4481..5221)
                     /gene="pmtD"
                     /locus_tag="PPY92_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtD"
                     /protein_id="HDJ2626080.1"
                     /translation="MRILNLVKYDFYSIFKSPLTYLAILVVSSLIATQSILMANSMDN
                     PKHIIVYGSVFAAAKWLLLIIGLMFVVKTITRDFSQGTIQLYMSKVKTRVGYIISKTI
                     SIILISILFALIHYVILIVVQASSNGKNLAFSKYVDNLWFFLIFLLFFGLFLFLITLA
                     SQKTAMIFSLGVFLVLIVPFIKPFITFIPRYGEKVLDAFDYIPFAYLTDKMISSNFDF
                     SNWQWVISLGSIVIFFILNILYVAKKDI"
     gene            complement(5221..6093)
                     /gene="pmtC"
                     /locus_tag="PPY92_002098"
     CDS             complement(5221..6093)
                     /gene="pmtC"
                     /locus_tag="PPY92_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtC"
                     /protein_id="HDJ2626081.1"
                     /translation="MKLEHITKKYGSNVVLNDIDFDFGDSRIVGLIGKNGVGKTTIMK
                     VMNGNIIKFDGKVDIDNADNIGFLIEHPKLYDNKSGLYNLKLFAQVLGKGFDKAYTDK
                     IIDAFGMRPYIKKKVKKYSMGMKQKLAIAVSLMNKPKFLILDEPTNGMDPDGSIDVLT
                     TIKSLVNELDMRILISSHKLEDIELICDRAVFLRDGHFVQDVNMEVGVASDTTIVTVD
                     HKDFDRTEKYLAEHFQLQNVDKADGHLMINAQKNYQVILKALSELDIYPKYIETRKSS
                     LRDTYFNINQRGDK"
BASE COUNT         2206 a         1031 c          769 g         2136 t
ORIGIN      
        1 gggatttatg tcccagcctc taaatgttat tagttatttt tactttcaat atcagaaata
       61 atttctttag tttttctttc aatatcttca aaatcttttt taaagatata agcaaatgct
      121 actacaccaa caccagcaac taatgttgta atagcaacta agctaaatac gaatttaaac
      181 acacatttaa taaaattcca catctcaatt tcacctctgt taaattaaat acctatctac
      241 atttcattat actatcatat atagaataat cattaaataa atacccactt ttacgaaata
      301 tattcatcaa attatatagt gagcaagcta atgattattt tcaaaaatgc cgaggaacct
      361 ctgtttcaac gtgacatgac cttatatgct aatgttaaca atgactatta tcgtgaatgc
      421 taacttaaga ttgttattta tgataagatg attttaagtt tttaacttaa tatccccacc
      481 atctataaaa atatgtttta actgattgat aagtcttata caccgagaaa tcacctcaaa
      541 caattttata tttcatttag taaaatataa ctttatttaa catgacattc aatatacata
      601 cgattactat ttatttacaa tatattacaa ctaaaaagaa aggggccgat gatatgcaac
      661 aagtgatatc agatattatg acttttagag gttcacattt cgagttaggt gttaaaactg
      721 gtaaatggct tcaacaaaca cctcttctaa aaaatcgaga aaaagaatgg aaaaaacgtg
      781 tcccacgttt cgatatagat gtaaatgaaa cttatcaaat attccaaact tacgcacctc
      841 aaatatggga agaattaatg ggactacaaa gtatactcaa aatgccaaca cgccaaatta
      901 ttttaaactt tggacattat cgttttaccg atttaaaaga aagtggttgc acggtctttc
      961 aaggcaaaga cttcatggtt agaaattatg attaccaccc tgcaacttat gatggtcgct
     1021 acttactata tcaacctaca gatagcggtt tagctcaaat tggtcctgta tcaagggtaa
     1081 ctggaagaat ggatggtatg aatgagtcag gattaactat gggctataac tttatgcacc
     1141 ggaagaaacc agcaaatggt tttgtatgtt atatgattgg tcgtctaatt cttgaaaatt
     1201 gccgaaatgt aacagaagca atacaattat tgaaagaaat accacacaga agctcattta
     1261 gctacatact gatggacaaa tcattgaacc atgcaattgt tgaagtgaca ccgagatcaa
     1321 tagatgttcg ttatgataat atatgtacaa atcattttga aatattaaca catgaaaatc
     1381 gtaattatac aaaagaatct aaagaaagac tggcgcgtac catttcacaa actaatgata
     1441 atttagatat gactacagca tttaagttat tcaataatcc tcagtatgaa atttacagta
     1501 aactattcaa aagctggtct ggcaccatcc atacatcaat gtatcaacct gaaacattaa
     1561 ctgcatattt tacattggga gaaaatgcgc caccagaaat aatagatttc aaatcatggt
     1621 tagatggtca agaccttaat atcactcact ttactggcaa tatagatacc gacttaactt
     1681 ttgccaacaa ataaataaca ccgatattag agaaattatg cttcctaata tcggtgctta
     1741 catcctttat ttattcaact ttgatattac catcaatcgt ataaaattct aaaacattgt
     1801 cgcttttccc aactttacca ttagtaaatg ttttattttt aactacgcta tcacccgttc
     1861 ccggatttag ctttagtata gtgtcttcag gtttactatc atacttaaaa cgaatatctc
     1921 cttgtttagt agaagctttt gcatctactt ttgatggcat gtttttaaat tcaatgtcac
     1981 catgatcgtt taaaaatatg gattgtttaa tttgactctt gtcgatttgt agcgtaccaa
     2041 taacaacttt aatattgcta tttttaatgt tacttttact aatataagta gaactattat
     2101 ttgtctttga atctagtgcg tcaagatttg agtttttaat aactacttca ccgttaatgc
     2161 tttgaatact tgtgttctct aaatcaacat cactgattct aacacttcct gctccagatg
     2221 atagatttaa acgtttaatc attttatcag gcatttcaat cgttaacgtt ttcttattct
     2281 catgaaaagg attcatatca attgcatatc ctcttgtctt agacctttta tcactaatct
     2341 tcaacgtctt atctactata ttaattaata tatcattgtc accatcatat ttaactctaa
     2401 attgattccc ccgttttata cgaagttctg tactatccaa atttatagat acattgtcaa
     2461 aagcatcgtc tttgaatgtt ttatcatatt gtttagtacc atatttgttt ttatcatagc
     2521 tgaaccaaat aatggttgct gctgtaaaaa agacaacaaa cactaaaagc ccaataaaaa
     2581 agaatttctt cattttctaa cgcttccttt gacagtttta atataccaaa gtaaatattt
     2641 taagattaaa cgataaatgt attctaaaat cttaaatatg acaatgataa atactaatcc
     2701 taatcctgaa tatgatatgg caaaaaatac attactaatt gagtctacaa ttcctctgga
     2761 tattgctgat gctaataaaa taattggaca aaacagcagc aaaagtgata ttaaaaataa
     2821 tgctaataca aataatccaa ctatagatac tggtataagg atgacaaaaa tagatagaat
     2881 gcctaaactt aatgaagcag caacagctct tgaaatattt tcaaatgttg gtttagattc
     2941 agcataatca ataacacttc tagcttttgt ttcagctgca acatcttgtg ggtcttttaa
     3001 cttctttaaa atttcccctt catcaacgcc attaagttct tgctcataaa aataacgttc
     3061 atattcatac attttttgat ctcttaaatg cctaggtagc gcatcaagtg ctgtttctaa
     3121 ttcatttaaa taagtaatct tgttcatata ggtcactcat cccaataaaa ttatttattc
     3181 gttttttata actttaagac ctaaagctat ttactaaaaa attcaatacg tttaatttct
     3241 aaatcttgat aaagtaagtt taattttaaa tggttatctc tatcaatata ctttaccgat
     3301 gagaaatcat tgccaacttt tgttctttta tatccgaatc caagattttc aatcgcgtta
     3361 tttatcggcg aaccaattcc cccatcaaaa ttagtatgta tcttttcatc tcttactaat
     3421 attatcccct taacgatgtt atccttttct ctcactttaa cggtaagatc agggtgagct
     3481 ttactgttat aaaatttaaa acgatcaaac ttaatttttt tatttacttc atagcctttt
     3541 ttatcaaact tctcatttaa tttaatatcg tttatttcaa gattagacaa atccacagtt
     3601 ttcgtatgac gatattgata taaaccaatt aatacaccaa aagaacagat taaaaataat
     3661 ataaacacaa cccagcgatt ttgaatacgc actgatgtca ccctttcttt gtacttattt
     3721 aaatatagca taaggtgcct cccgtttcat ccttaacaaa aattaacaaa gaatttgcaa
     3781 ttaatttatt tcattacctt ttattactat aaattggtat aatgttgtaa atgctaaagg
     3841 aggtttttac aatgacaaat ttagaaactt atttcaaaaa tagccaaccc ttaaatgaat
     3901 acattgatgg aatgaagaca aatcaagata ccgttctcag tatctatcaa tcatttgagt
     3961 taccagaaga tgattcacgc atcaaaaaaa taaaagagat gaattattct aaagtattag
     4021 taattactga agactggtgt ggagatgcaa tgatgaatct cccaattcta aaacacatca
     4081 gtgaagcatt aaatttagaa gtacgcgtgt tccatagaga tgatgataca aaactgatcg
     4141 atcaatattt aacaaatggt aaatcacgtg caattcctat cttcgtattc ttgaatgatc
     4201 aattcgaaca agaaacagtt tggggaccaa gagcaaatga agtccaaaaa tttgtaactg
     4261 atgttcgtgc tgataaatta ccgagcaaag atcatccaga ctatgcagat ttagaaaaag
     4321 aaacacactt agttatatca aaccgctata aaactgacgc ttcattttgg aaagcagttt
     4381 ataattccat cctaaataaa ttagaaaata aataaattta caataagaca aactttgagg
     4441 ttgggaaaat ttaaaattcc caacctcaaa attattttta ttaaatgtct tttttagcga
     4501 catataagat attcaaaatg aagaatatca ctatagaacc taatgaaatt acccattgcc
     4561 aattgctaaa atcaaagtta gagctaatca ttttatcagt taagtaagca aaagggatat
     4621 aatcaaaagc atctaaaact ttttcaccgt atcttgggat aaatgtaata aaaggtttaa
     4681 taaacggtac aatgagtact aaaaatacac ctaatgaaaa tatcattgct gttttttgtg
     4741 atgcaagtgt gattaagaat aaaaacaagc caaagaatag taaaaagatt aggaagaacc
     4801 ataaattatc tacatattta gaaaacgcca aattttttcc attactagat gcctgcacaa
     4861 caatcaaaat cacataatga attaatgcaa ataatattga aattaaaata attgaaattg
     4921 ttttcgaaat aatgtatcca acgcgtgttt taactttact catatatagt tgaattgtac
     4981 cttgtgaaaa atcacgcgta attgtcttaa caacaaacat taatccaatt attaacaata
     5041 accattttgc tgcagcaaat acagatccat agacaataat atgtttcggg ttatccatcg
     5101 aatttgccat aagtatactt tgagttgcaa tcaaactaga tacgactagt atcgctaaat
     5161 atgttaaagg acttttaaat atactataaa aatcatactt aactaaattt aaaattctca
     5221 ttatttatca cctctttgat ttatattgaa gtacgtatca cgcaatgaac ttttacgtgt
     5281 ttcaatatat ttcggataaa tatctaattc agataatgct tttagtataa cttgataatt
     5341 tttttgtgca ttgatcatta aatgtccgtc tgctttgtcg acattttgta attggaaatg
     5401 ctctgcaaga tatttttcag ttctatcaaa gtctttatga tcaacagtaa ctatcgttgt
     5461 gtcagatgca acacctacct ccatgtttac atcttgaaca aaatgtccgt ctcttaaaaa
     5521 tacagctcta tcacaaatta attcaatatc ttctaactta tgacttgata ttagaattct
     5581 catatcaagt tcatttacta aagacttaat tgtagtcagc acatcaattg agccatctgg
     5641 atccatacca tttgtaggct catccaagat taaaaattta ggtttattca ttaaagatac
     5701 tgcaattgct aacttttgct tcatccccat tgaatatttc ttaactttct ttttaatata
     5761 aggtctcata ccaaatgcat ctataatttt gtctgtgtat gctttatcaa aacccttacc
     5821 taatacttgt gcaaataatt tcaagttata caatcctgat ttattatcat ataatttagg
     5881 atgctcaatt aaaaaaccga tattatctgc attatcaata tctacttttc catcaaattt
     5941 aataatatta ccattcatta ctttcataat ggttgtttta ccaacaccgt tttttcctat
     6001 taatccgaca attctactat cgccaaagtc aaaatcaata tcatttaaaa cgacatttga
     6061 gccgtatttt tttgtaatat gttctaattt catcgactgc ctcctttaaa ttttctagta
     6121 tttattttat tgagctgttt aa
//
LOCUS       DANBWH010000158         6100 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.164, whole genome shotgun sequence.
ACCESSION   DANBWH010000158 DANBWH010000000
VERSION     DANBWH010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.164"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            129..830
                     /locus_tag="PPY92_002099"
     CDS             129..830
                     /locus_tag="PPY92_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDJ2626082.1"
                     /translation="MARKVVVVDDEKPIADILEFNLKKEGYDVYCAYDGNDAVDLIYE
                     EEPDIVLLDIMLPGRDGMEVCREVRKKYEMPIIMLTAKDSEIDKVLGLELGADDYVTK
                     PFSTRELIARVKANLRRHYSQPAQDTGNVTNEITIKDIVIYPDAYSIKKRGEDIELTH
                     REFELFHYLSKHMGQVMTREHLLQTVWGYDYFGDVRTVDVTIRRLREKIEDDPSHPEY
                     IVTRRGVGYFLQQHE"
     gene            843..2669
                     /gene="walK"
                     /locus_tag="PPY92_002100"
     CDS             843..2669
                     /gene="walK"
                     /locus_tag="PPY92_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HDJ2626083.1"
                     /translation="MKWLKQLQSLHTKLVIVYVLLIIIGMQIIGLYFTNNLEKELLDN
                     FKKNITQYAKQLEISIEKVYDEKGSVNAQKDIQNLLSEYANRQEIGEIRFIDKDQIII
                     ATTKQSNRSLINQKANDSSVQKALSLGQSNDHLILKDYGGGKDRVWVYNIPVKVDKKV
                     IGNIYIESKINDVYNQLNNINQIFIVGTAISLLITVILGFFIARTITKPITDMRNQTV
                     EMSRGNYTQRVKIYGNDEIGELALAFNNLSKRVQEAQANTESEKRRLDSVITHMSDGI
                     IATDRRGRIRIVNDMALKMLGMAKEDIIGYYMLSVLSLEDEFKLEEIQENNDSFLLDL
                     NEEEGLIARVNFSTIVQETGFVTGYIAVLHDVTEQQQVEHERREFVANVSHELRTPLT
                     SMNSYIEALEEGAWKDEELAPQFLSVTREETERMIRLVNDLLQLSKMDNESDQINKEI
                     IDFNMFINKIINRHEMSAKDTTFIRDIPKKTIFTEFDPDKMTQVFDNVITNAMKYSRG
                     DKRVEFHVKQNPLYNRMTIRIKDNGIGIPINKVDKIFDRFYRVDKARTRKMGGTGLGL
                     AISKEIVEAHNGRIWANSVEGQGTSIFITLPCEVIEDGDWDE"
     gene            2662..3996
                     /locus_tag="PPY92_002101"
     CDS             2662..3996
                     /locus_tag="PPY92_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626084.1"
                     /translation="MNNKEHIKSVILALLVLMSVVLTYMVWNFSPDIANVDNTDSKKS
                     ETKPLTTPMTAKMDTTITPFQIIHSKNDHPEGTIATVSNVNKLTKPLKNKEVKSVEHV
                     RRDHNLMIPDLSSDFTLFDFTYDLPLSTYLGQVLNMNAKVPNHFNFNRLVIDHDADDN
                     IVLYAISKDRHDYVKLTTTTKNDHFLDALAAVKKDMQPYTDIITNKDTIDRTTHVFAP
                     SKPEKLKTYRMVFNTISVEKMNAILFDDSTIVRSSKSGVTTYNNNTGVANYNDKNEKY
                     HYKNLSEDEASSSKMEETIPGTFDFINGHGGFLNEDFRLFSTNNQSGELTYQRFLNGY
                     PTFNKEGSNQIQVTWGEKGVFDYRRSLLRTDVVLNSEDNKTLPKLESVRSSLANNSDI
                     NFEKVTNIAIGYEMQDNSDHNHIEVQINSELVPRWYVEYDGEWYVYNDGRLE"
     gene            3997..4785
                     /locus_tag="PPY92_002102"
     CDS             3997..4785
                     /locus_tag="PPY92_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HDJ2626085.1"
                     /translation="MNWKLTKTLFIFVFILVNIVLVSIYVNKVNRSHINEVESNNEVN
                     FQQEEIKVPASILNKSVKGIKLEQITGRSKDFSSKAKGDSDLTTSDGGKLLNANISQS
                     VKVSDNNLKDLKDYVNKRVFKGSEYQLSEINSGSVKYEQTYDNFPILNNSKAMLNFNI
                     EDNKAASYKQSMMDDIKPTDGADKKHQVIGVRKAIEALYYNRYLKKGDEVINARLGYY
                     SVVNETNVQLLQPNWEIKVKHDGKDKTNTYYVEATNNNPKIINH"
     gene            5174..5974
                     /locus_tag="PPY92_002103"
     CDS             5174..5974
                     /locus_tag="PPY92_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDJ2626086.1"
                     /translation="MSRLIRMSVLASGSTGNATFVENEKGSLLVDVGLTGKKMEELFS
                     QIDRNIQDLNGILVTHEHIDHIKGLGVLARKYQLPIYANEKTWQAIEKKDSRIPMDQK
                     FIFNPYETKSIAGFDVESFNVSHDAIDPQFYIFHNNYKKFTILTDTGYVSDRMKGMIR
                     GSDAFIFESNHDVDMLRMCRYPWKTKQRILGDMGHVSNEDAGHAMTDVITGNTKRIYL
                     SHLSQDNNMKDLARMSVGQVLNEHDIDTEKEVLLCDTDKAIPTPIYTI"
BASE COUNT         2192 a          899 c         1215 g         1794 t
ORIGIN      
        1 aagacggaaa atgcgcacaa taacaaaaat agtaagcgac atcctgtgat tttttacaca
       61 aacataaacg ataaagaaca aaaaatgata aaataatatt aatgatttaa gaaaagaggt
      121 ttatgcaaat ggctagaaaa gttgttgtag ttgatgatga aaaaccgatt gctgatattt
      181 tagaatttaa cttaaaaaaa gaaggatacg atgtgtactg tgcatacgat ggtaatgatg
      241 cagtcgactt aatttatgaa gaagaaccag acatcgtatt actagatatc atgttacctg
      301 gtcgtgatgg tatggaagta tgtcgtgaag tgcgcaaaaa atacgaaatg ccaataataa
      361 tgcttactgc taaagattca gaaattgata aagtgcttgg tttagaacta ggtgcagatg
      421 actatgtaac gaaaccgttt agtacgcgtg aattaatcgc acgtgtgaaa gcgaacttac
      481 gtcgtcatta ctcacaacca gcacaagaca ctggaaatgt aacgaatgaa atcacaatta
      541 aagatattgt gatttatcca gacgcatatt ctattaaaaa acgtggcgaa gatattgaat
      601 taacacatcg tgaatttgaa ttgttccatt atttatcaaa acatatggga caagtaatga
      661 cacgtgaaca tttattacaa acagtatggg gctatgatta ctttggcgat gtacgtacgg
      721 tcgatgtaac gattcgtcgt ttacgtgaaa agattgaaga tgatccgtca catcctgaat
      781 atattgtgac gcgtagaggc gttggatatt tcctccaaca acatgagtag aggtcgaaac
      841 gaatgaagtg gctaaaacaa ctacaatccc ttcatactaa acttgtaatt gtttatgtat
      901 tactgattat cattggtatg caaattatcg ggctgtattt tacaaataat cttgaaaaag
      961 agctgcttga taattttaag aagaatatta cgcagtacgc taaacaatta gaaattagta
     1021 ttgaaaaagt atatgacgaa aagggctccg taaatgcaca aaaagatatt caaaatttat
     1081 taagtgagta tgccaaccgt caagaaattg gagaaattcg ttttatagat aaagaccaaa
     1141 ttattattgc gacgacgaag cagtctaacc gtagtctaat caatcaaaaa gcgaatgata
     1201 gttctgtcca aaaagcacta tcactaggac aatcaaacga tcatttaatt ttaaaagatt
     1261 atggcggtgg taaggaccgt gtctgggtat ataatatccc agttaaagtc gataaaaagg
     1321 taattggtaa tatttatatc gaatcaaaaa ttaatgacgt ttataaccaa ttaaataata
     1381 taaatcaaat attcattgtt ggtacagcta tttcattatt aatcacagtc atcctaggat
     1441 tctttatagc gcgaacgatt accaaaccaa tcaccgatat gcgtaaccag acggtcgaaa
     1501 tgtccagagg taactatacg caacgtgtga agatttatgg taatgatgaa attggcgaat
     1561 tagctttagc atttaataac ttgtctaaac gtgtacaaga agcgcaggct aatactgaaa
     1621 gtgagaaacg tagactggac tcagttatca cccatatgag tgatggtatt attgcaacag
     1681 accgccgtgg acgtattcgt atcgtcaatg atatggcact caagatgctt ggtatggcga
     1741 aagaagacat catcggatat tacatgttaa gtgtattaag tcttgaagat gaatttaaac
     1801 ttgaagaaat tcaagagaat aatgatagtt ttttattaga tttaaatgaa gaagaaggtc
     1861 taatcgcacg tgttaacttt agtacgattg tgcaggaaac aggatttgta actggttata
     1921 tcgctgtgtt acatgacgtt actgaacaac aacaagttga acatgagcgt cgtgaatttg
     1981 ttgccaatgt atcacatgag ttacgtacac ctttaacttc tatgaatagt tacattgaag
     2041 cacttgaaga aggtgcatgg aaagatgagg aacttgcgcc acaattttta tctgttaccc
     2101 gtgaagaaac agaacgaatg attcgactgg tcaatgactt gctacagtta tctaaaatgg
     2161 ataatgagtc tgatcaaatc aacaaagaaa ttatcgactt taacatgttc attaataaaa
     2221 ttattaatcg acatgaaatg tctgcgaaag acacaacatt tattcgagat attccgaaaa
     2281 agacgatttt cacagaattt gatcctgata aaatgacgca agtattcgat aatgtcatta
     2341 caaatgcgat gaaatattct agaggcgata aacgtgtcga gttccacgtg aaacaaaatc
     2401 cactttataa tcgaatgacg attcgtatta aagataatgg catcggtatt cctatcaata
     2461 aagtcgataa gatattcgac cgattctatc gtgtagataa ggcacgtacg cgtaaaatgg
     2521 gtggtactgg attaggacta gccatttcga aagagattgt ggaagcgcac aatggtcgta
     2581 tttgggcaaa cagtgtagaa ggtcaaggta catctatctt tatcacactt ccatgtgaag
     2641 tcattgaaga cggtgattgg gatgaataat aaggaacata ttaaatctgt cattttagcg
     2701 ctactcgtct tgatgagtgt cgtattgaca tatatggtat ggaacttttc tcctgatatt
     2761 gcaaatgtcg acaatacaga tagtaagaag agtgaaacga aacctttaac gacacctatg
     2821 acagccaaaa tggatacaac tattacgcca tttcagatta ttcattcgaa aaatgatcat
     2881 ccagaaggta cgattgcgac ggtatctaat gtgaataaac tgacgaaacc tttgaaaaat
     2941 aaagaagtga agtccgtgga acatgttcgt cgtgatcata acttgatgat tccggattta
     3001 agcagtgatt ttacattatt cgattttacg tatgatttac cgttatcaac atatcttggc
     3061 caagtactga acatgaatgc gaaagtacca aatcatttca atttcaatcg tttagtcata
     3121 gatcatgatg ctgatgataa tatcgtgctt tatgctataa gtaaagaccg ccacgattac
     3181 gtaaaattaa caactacaac gaaaaatgat cattttttag atgcattagc agcagtgaaa
     3241 aaagatatgc aaccatacac agatatcatc acaaacaaag atacaattga tcgtacgacg
     3301 catgtttttg caccaagtaa acctgaaaag ttaaaaacat atcgcatggt atttaacacg
     3361 attagtgttg agaaaatgaa tgctatacta tttgacgatt caaccatcgt tcgtagttca
     3421 aagagtggtg ttacaaccta caacaataat acaggtgtcg caaactataa cgataaaaat
     3481 gaaaaatatc attataaaaa cctgtccgaa gatgaagcaa gttccagcaa aatggaagaa
     3541 acgattccag gaacctttga ttttattaat ggtcatggtg gtttcttaaa cgaagacttc
     3601 agattgttta gtacgaataa tcagtcaggc gagttaacat atcaacgttt ccttaacggt
     3661 tatccaacgt ttaataaaga aggctctaat caaattcaag tcacttgggg tgaaaaaggc
     3721 gtctttgatt atcgtcgttc gttattgcgc accgacgttg tgttaaatag tgaggataat
     3781 aaaacgttgc cgaaattaga gtctgtacgt tcaagcttag cgaacaatag tgatattaat
     3841 tttgaaaaag taacaaacat cgctatcggt tacgaaatgc aggataactc agatcataat
     3901 cacattgaag tgcagattaa cagtgaactc gtaccgcgtt ggtatgtaga atatgatggt
     3961 gaatggtatg tttataacga tgggaggctt gaataaatga actggaaact gacaaagaca
     4021 cttttcattt tcgtgtttat tcttgtcaac atcgtgttag tatcgattta tgttaataaa
     4081 gtcaatcgct cacacattaa tgaagttgag agtaacaatg aagttaattt tcagcaagaa
     4141 gaaattaaag taccggctag tatattgaat aaatcagtta aaggtataaa attagagcaa
     4201 attacagggc gatcaaaaga ctttagttct aaagctaagg gcgattcgga tttgaccaca
     4261 tcagatggtg gaaaattatt gaatgcgaac attagtcaat cggtaaaggt cagtgacaat
     4321 aacttaaaag atttgaaaga ttatgttaac aaacgtgtgt tcaaaggttc agaatatcaa
     4381 ttaagtgaaa ttaattctgg ttctgtaaaa tacgaacaaa cgtatgataa tttcccgatt
     4441 ttgaacaata gtaaagcgat gttgaacttt aatatagaag ataacaaagc ggctagttat
     4501 aaacaatcaa tgatggatga cattaagccc acagatggtg cagataagaa gcatcaagtt
     4561 attggagtga gaaaagcaat cgaggcatta tattataatc gttacttgaa aaaaggtgat
     4621 gaagtcatta atgctagact cggttactac tcagtcgtga acgaaacgaa tgttcaattg
     4681 ttacaaccaa actgggaaat taaagtgaag catgacggta aggacaaaac gaatacttac
     4741 tatgtcgaag cgacaaataa taaccctaaa attattaatc attaatatga atcgtaataa
     4801 gctagtattg caagctcatc atatgtgaga agcggtgcta gctttttgct ggtatgggtt
     4861 ttcatggatg acatttctgc gtctctaatg tgcgcattta ttcatatttt aagtagaacc
     4921 gcattttaaa attaacgtaa ctgctatttt aaaaacttta gtatttgtct aatcattgtt
     4981 ataataatta agaaattcat tgcacgtgat tatcaaaatt taaatataag aaaccggtcg
     5041 atgaactaaa gttacataat aggaaaggta tacaaaacag ctaatatact gatagtttct
     5101 gtagggaaaa tcgtatattt gcactgatgt atattgcagt catatagaga gattgactgt
     5161 ttaaagagaa aggatgagcc gcttgatacg catgagtgta ttagcaagtg gtagtacagg
     5221 taacgccact tttgtagaaa atgaaaaagg tagtctatta gttgatgttg gtttgactgg
     5281 caagaaaatg gaagaattgt ttagtcaaat tgaccgtaat attcaagatt taaatggtat
     5341 tttagtaacc catgaacata ttgatcatat taaaggatta ggtgttttgg cgcgtaaata
     5401 tcaattgcca atttatgcga atgaaaagac ttggcaggca attgaaaaga aagatagtcg
     5461 catccctatg gatcagaaat tcatttttaa tccttatgaa acaaaatcta ttgcaggttt
     5521 cgatgttgaa tcgtttaacg tgtcacatga tgcaatagat ccgcaatttt atattttcca
     5581 taataactat aagaagttta cgattttaac ggatacgggt tacgtgtctg atcgtatgaa
     5641 aggtatgata cgtggcagcg atgcgtttat ttttgagagt aatcatgacg tcgatatgtt
     5701 gagaatgtgt cgttatccat ggaagacgaa acaacgtatt ttaggcgata tgggtcatgt
     5761 atctaatgag gatgcgggtc atgcgatgac agatgtgatt acaggtaaca cgaaacgtat
     5821 ttacctatcg catttatcac aagacaataa catgaaagat ttggcgcgta tgagtgttgg
     5881 ccaagtattg aacgaacacg atattgatac ggaaaaagaa gtattgctat gtgatacgga
     5941 taaagctatt ccaacgccaa tatatacaat ataaatgaga gtcaccctat aaagttcggc
     6001 actgctgtga gacgacttta tcgggtgctt ttttatgtta ttggtgggaa atggctgttg
     6061 ttggaattaa ggttctattt gaaatgtaaa aaataattcg
//
LOCUS       DANBWH010000159         5967 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.165, whole genome shotgun sequence.
ACCESSION   DANBWH010000159 DANBWH010000000
VERSION     DANBWH010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.165"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            616..1527
                     /gene="rapZ"
                     /locus_tag="PPY92_002104"
     CDS             616..1527
                     /gene="rapZ"
                     /locus_tag="PPY92_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDJ2626087.1"
                     /translation="MDNNEKEKSKSELLVVTGLSGAGKSLVIQCLEDMGYFCVDNLPP
                     VLLPKFVELMEQGNPSLRKVAIAIDLRGKELFNSLVAVVDKVKSESDVIIDVMFLEAS
                     TEKLISRYKETRRAHPLMEQGKRSLINAINDEREHLSQIRSIANFVIDTTKLSPKELK
                     ERIRRYYEDEEFETFTINVTSFGFKHGIQMDADLVFDVRFLPNPYYVVDLRPLTGLDK
                     DVYNYVMKWKETEIFFEKLTDLLDFMIPGYKKEGKSQLVIAIGCTGGQHRSVALAERL
                     GNYLNEVFEYNVYVHHRDAHIERGEKK"
     gene            1524..2519
                     /locus_tag="PPY92_002105"
     CDS             1524..2519
                     /locus_tag="PPY92_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDJ2626088.1"
                     /translation="MRQIKVVLIGGGTGLSVMARGLREFPIDITAIVTVADNGGSTGK
                     IRDEMDIPAPGDIRNVIAALSDSESVLSQLFQYRFEENQISGHSLGNLLIAGMTNITN
                     DFGHAIKALSKILNIKGRVIPSTNTSVQLNAVMEDGEIVFGETNIPKKHKKIDRVFLE
                     PNDVQPMEEAIDALREADLIVLGPGSLYTSVISNLCVNGISDALIHSDAPKLYVSNVM
                     TQPGETDGYSVKDHIDAIHRQAGQPFIDYVICSTQTFNAQVLKKYEEKHSKPVEVNKA
                     ELEKESINVKTSSNLVEISENHLVRHNTKVLSTMIYDIALELISTIPFVPSDKRK"
     gene            2630..3574
                     /gene="whiA"
                     /locus_tag="PPY92_002106"
     CDS             2630..3574
                     /gene="whiA"
                     /locus_tag="PPY92_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDJ2626089.1"
                     /translation="MSFASEMKNELTRIDVDEMNAKAELSALIRMNGALSLSNQQFVI
                     NVQTENATTARRIYSLIKRVFNVEVEILVRKKMKLKKNNIYICRTKMKAKEILDELGI
                     LKDGIFTHEIDHSMIQDDEMRRSYLRGAFLAGGSVNNPETSSYHLEIFSQNESHAEGL
                     TKLMNSYELNAKHLERKKGSITYLKEAEKISDFLSLIGGYQALLKFEDVRIVRDMRNS
                     VNRLVNCETANLNKTVSAAMKQVESIKLIDKEIGIENLPDRLREIARIRVEHQEISLK
                     EIGEMVSTGPISKSGVNHRLRKLNDLADKIRNGEQIEL"
     gene            4138..4725
                     /gene="clpP"
                     /locus_tag="PPY92_002107"
     CDS             4138..4725
                     /gene="clpP"
                     /locus_tag="PPY92_002107"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDJ2626090.1"
                     /translation="MNLIPTVIETTNRGERAYDIYSRLLKDRIIMLGSQIDDNVANSI
                     VSQLLFLQAQDSEKDIYLYINSPGGSVTAGFAIYDTIQHIKPDVQTICIGMAASMGSF
                     LLAAGAKGKRFALPNAEVMIHQPLGGAQGQATEIEIAANHILKTREKLNRILSERTGQ
                     SIEKIQKDTDRDNFLTAEEAKEYGLIDEVMVPETK"
     gene            complement(4968..5870)
                     /locus_tag="PPY92_002108"
     CDS             complement(4968..5870)
                     /locus_tag="PPY92_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDJ2626091.1"
                     /translation="MKQYLITGGTGMVGSQLVNEIKKSDSHITILTRHDQISNDKKIS
                     YVNWAKSGWEHKVPQNIDVVINLAGATLNKRWTPEYKQTLMLSRIQSTQALYELFKSR
                     NKAPKVLFNASATGYYPPDLFMSYTEVYKTLPFDFLSDIVYQWERFAQQFEQLGTRVV
                     IGRFGMILSNEGGALQTMKLPYKYYIGGKLGSGQQWYSWIHINDLIQAILFLINNESA
                     SGPFNLTAPIPERQNLFGYTLARAMHKPHETWAPSLAMRLILGQMSTVVLDTQKVLPN
                     KIQALGFQFKYSNLKMALEDLIKE"
BASE COUNT         2131 a          851 c         1104 g         1881 t
ORIGIN      
        1 tgtgtactta aaaataggaa tacatgagta aaactcatgc ataagaaata ctaatttcta
       61 tagaaaaagt attactttat cgttgtacca ccccaactcg cattgcctgt agaatttctt
      121 ttcgaaattc tctttgttgg ggcccacacc ccaacttgca ttgtctgtag aaattggaaa
      181 tccaatttct ctgtgttggg gcccacaccc caacttgcat tgtctgtaga aattggaaat
      241 ccaatttctc tgtgttgggg cccacacccc aactcgcatt gcctgtagaa tttcttttcg
      301 aaattctctg tgttggggcc cacaccccaa cttgcattgt ctgtagaaat tggaaatcca
      361 atttctctgt gttggggccc ctgactagag ttgaaaaaag cttgttgcaa gcgcattttc
      421 attcagtcaa ctactagcaa tataatatta tagaccctag gacattgatt tatgtcccaa
      481 gctcctttaa atgatgtata tttttagaaa tttaatctag acatagttgg aaataaatat
      541 aaaacatcgt tgcttaattt tgtcatagaa catttaaatt aacatcatga aattcgtttt
      601 ggcggtgaaa aaataatgga taataatgaa aaagaaaaaa gtaaaagtga actattagtt
      661 gtaacaggtt tatctggagc aggtaaatct ttggttattc aatgtttaga agacatggga
      721 tatttttgtg tagataatct accaccagtg ttattgccta aatttgtaga gttgatggaa
      781 caaggaaatc catccttaag aaaagtggca attgcaattg atttaagagg taaggaacta
      841 tttaattcat tagttgcagt agtggataaa gttaaaagtg aaagtgacgt catcattgat
      901 gttatgtttt tagaagcaag tactgaaaaa ttaatttcaa gatataaaga aacgcgtcgt
      961 gcacatcctt tgatggaaca aggtaaaaga tccttaatta atgcaattaa tgatgagcga
     1021 gagcatttgt ctcaaattag aagtatagct aattttgtta tagatactac aaagttatca
     1081 cctaaagaat taaaagaacg cattcgtcga tactatgaag atgaagagtt tgaaactttt
     1141 acaattaatg tcacaagttt cggttttaaa catggaattc agatggatgc agatttagta
     1201 tttgatgtac gatttttacc aaatccatat tatgtagtag atttaagacc tttaacagga
     1261 ttagataaag acgtttataa ttatgttatg aaatggaaag agacggagat tttctttgaa
     1321 aaattaactg atttgttaga ttttatgata cccgggtata aaaaagaagg gaaatctcaa
     1381 ttagtaattg ccatcggttg tacgggtgga caacatcgat ctgtagcatt agcagaacga
     1441 ctaggtaatt atctaaatga agtatttgaa tataatgttt atgtgcatca tagggacgca
     1501 catattgaaa ggggcgagaa aaaatgagac aaataaaagt tgtacttatc ggtggtggca
     1561 ctggcttatc agttatggct aggggattaa gagaattccc aattgatatt acggcgattg
     1621 taacagttgc tgataatggt gggagtacag ggaaaatcag agatgaaatg gatataccag
     1681 caccaggaga catcagaaat gtgattgcag ctttaagtga ttctgagtca gttttaagcc
     1741 aactttttca gtatcgcttt gaagaaaatc aaattagcgg tcactcatta ggtaatttat
     1801 taatcgcagg tatgactaat attacgaatg atttcggaca tgccattaaa gcattaagta
     1861 aaattttaaa tattaaaggt agagtcattc catctacaaa tacaagtgtg caattaaatg
     1921 ctgttatgga agatggagaa attgtttttg gagaaacaaa tattcctaaa aaacataaaa
     1981 aaattgatcg tgtgttttta gaacctaacg atgtgcaacc aatggaagaa gcaatcgatg
     2041 ctttaaggga agcagattta atcgttcttg gaccagggtc attatatacg agcgttattt
     2101 ctaacttatg tgtcaatggt atttcagatg cgttaattca ttctgatgcg cctaagctat
     2161 atgtttctaa tgtgatgacg caacctgggg aaacagatgg ttatagcgtg aaagatcata
     2221 tcgatgcgat tcatagacaa gctggacaac cgtttattga ttatgtcatt tgtagtacac
     2281 aaactttcaa tgctcaagtt ttgaaaaaat atgaagaaaa acattctaaa ccagttgaag
     2341 ttaataaggc tgaacttgaa aaagaaagca taaatgtaaa aacatcttca aatttagttg
     2401 aaatttctga aaatcattta gtaagacata atactaaagt gttatcgaca atgatttatg
     2461 atatagcttt agaattaatt agtactattc ctttcgtacc aagtgataaa cgtaaataat
     2521 atagaacgta atcatattat gatatgataa tagagctgtg taaaaaaatg aaaatagaca
     2581 gtggttctaa ggtgaatcat gttttaaata agaaaggaat gactgtacga tgagctttgc
     2641 atcagaaatg aaaaatgaat taactagaat agacgtcgat gaaatgaatg caaaagcaga
     2701 gctcagtgcg ctgattcgaa tgaatggtgc acttagtctt tcaaatcaac aatttgttat
     2761 aaatgttcaa acggaaaatg caacaacggc aagacgtatt tattcgttga ttaaacgtgt
     2821 ctttaatgtg gaagttgaaa tattagtccg taaaaaaatg aagcttaaaa aaaataatat
     2881 ttatatttgt cgtacaaaga tgaaagcgaa agaaattctt gatgaattag gaattttaaa
     2941 agacggcatt tttacgcatg aaattgatca ttcaatgatt caagatgatg aaatgagacg
     3001 cagttacttg agaggagctt ttctggcagg tggctcagtg aataaccctg aaacatcttc
     3061 gtaccatttg gaaatttttt ctcaaaatga gagtcatgca gaaggcttaa cgaaactaat
     3121 gaatagttat gagttgaatg ccaaacattt agagcgaaaa aaaggaagta ttacgtattt
     3181 aaaagaagcg gaaaagattt cggattttct tagtttgata ggtggctatc aagcgttatt
     3241 aaaatttgaa gacgtacgta ttgtaagaga tatgcgtaat tctgttaacc gactcgttaa
     3301 ttgtgaaacg gcaaatctaa ataaaacagt tagtgctgcg atgaaacaag ttgagagcat
     3361 taaattgatt gataaagaaa ttggtattga aaatttacca gacaggttga gagagattgc
     3421 tagaattcga gtagaacatc aagaaatttc gttgaaagag attggagaaa tggtatcaac
     3481 tggtccaatt tcaaaatcag gtgtaaatca ccgattaaga aaacttaatg atttagccga
     3541 taagattaga aatggtgaac aaatagaatt ataagtaaga aggtgttttt ggtagtgaat
     3601 tatcaaaaat acctttttta tgattaaaaa gtgtttaatg aataatagtc gactaactat
     3661 taaattgagc tggaaagttt aatgaaggaa attcaataac caagcgttac ctaattttct
     3721 cattaagtag catcatatgt atcaatttgg tcataagaaa atatgaatgt gataattaat
     3781 tgagtcactg atcatagaca aaaacaaaat cagcataaat aagaatcccg actttgcggg
     3841 attctctgta ttgaaaagta ttgtatttta ttaaaacagc ctccttgaag ggaattgaac
     3901 ccctatctta agaaccggaa tcttacgtgt tatccattac accacaagga gcaaatatgt
     3961 aatcatatct ctgtactatg agaagtaaaa taagtacaat ctaaaataga tattatcagt
     4021 ttacaaagga aagagaaaag cgtcaaacaa tgtaactatt taaagtcaaa gtgtttgacc
     4081 aaatttgact taatatgtaa aataatgagt aacagttatt acaaggagga aatatagatg
     4141 aatttaattc ctacagttat tgaaacaaca aaccgcggtg aacgtgcata tgatatatac
     4201 tcacgtttat taaaagaccg tattattatg ttaggttcac aaattgatga caacgtagca
     4261 aattcaatcg tatcacagtt attattctta caagcgcaag actcagagaa agatatttat
     4321 ttatacatta attcaccagg tggaagtgta acagctggtt ttgcgattta tgatacaatt
     4381 caacacatta aacctgatgt tcaaacaatt tgtatcggta tggctgcatc aatgggatca
     4441 ttcttattag cagctggtgc aaaaggtaaa cgtttcgcgt taccaaatgc agaagtaatg
     4501 attcaccaac cattaggtgg tgctcaagga caagcaactg aaatcgaaat tgctgcaaat
     4561 cacattttaa aaacacgtga aaaattaaac cgcattttat cagagcgtac tggtcaaagt
     4621 attgaaaaaa tacaaaaaga cacagatcgt gataacttct taactgcaga agaagctaaa
     4681 gaatatggct taattgatga agtgatggta cctgaaacaa aataattcaa agtaaagagt
     4741 agactaagca gtctgctctt tttgtatgag taaaccgagg tgtcaataaa ttgtttacta
     4801 tactttgagc ggaaatatga ttgaatgaag ctagttgaac cgtaactata tgaaatgttc
     4861 ccttcaaagt agacattgaa aggaacattt caatcctttg tttgtaagtc gctctagaca
     4921 ttacatttag tacatatgtt gtttctaatg ctcattaatg gtattgatta ttctttgatt
     4981 aaatcttcaa gtgccatttt taaattacta tatttaaatt ggaatcccaa tgcttgaatt
     5041 ttattaggta atactttttg agtatccaat actactgttg acatttgacc aagtatgaga
     5101 cgcattgcaa gacttggtgc ccaagtttca tggggcttat gcatagctct tgctaaagtg
     5161 tagccaaata aattttgacg ctcaggtata ggtgcagtta aattaaacgg accactagct
     5221 gactcgttat ttattaaaaa taaaatagct tgaattaaat cattgatatg aatccatgaa
     5281 taccattgtt gaccagaacc taatttacca ccaatgtaat atttgtatgg tagtttcatt
     5341 gtttgtaacg caccgccttc attcgataaa atcataccga aacgaccgat gacaactcgc
     5401 gtacctaatt gttcaaattg ttgtgcgaaa cgttcccatt gatacacaat atctgataag
     5461 aaatcaaatg gtaaagtttt ataaacttct gtgtaactca taaataaatc aggaggatag
     5521 taaccagtgg cgctagcatt aaataaaact ttaggtgctt tattacgtga tttaaacaat
     5581 tcatataaag cttgcgtaga ttgaattcta cttagcatta gcgtttgttt atattccggt
     5641 gtccatcgtt tattcagtgt agcacctgct aagttgatga ccacatcgat attttgagga
     5701 actttgtgtt cccacccaga tttagcccag ttgacatatg aaattttctt atcatttgaa
     5761 atttggtcgt gtcgcgttaa tatcgtgata tgtgaatctg attttttaat ttcattaact
     5821 aattgagatc caaccatacc agtcccacca gtaattaagt attgtttcat tatcattcac
     5881 cccatgtaat tttgtattta gttactattt aataatcatt attgttgttc aaaggttata
     5941 cattatttag acaataatat gtcaata
//
LOCUS       DANBWH010000160         5964 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.166, whole genome shotgun sequence.
ACCESSION   DANBWH010000160 DANBWH010000000
VERSION     DANBWH010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5964
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.166"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            99..863
                     /locus_tag="PPY92_002109"
     CDS             99..863
                     /locus_tag="PPY92_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDJ2626092.1"
                     /translation="MDINSEEYKQEVLIKDVVMLAARILLESGAEGTRVEDTMTRIAK
                     KLGYSESNSFVTNTVIQFTLHSESFPRIFRITSRDTNLIKISQANKISRQITNNEISL
                     AEAKTQLEKIYVAKRDSSLPFKGFAAAMIAMSFLYLQGGRLIDVLTAILAGSLGYLVT
                     EILDRKLHAQFIPEFIGSLVIGIIAVIGHTLIPTGDLATIIIAAVMPIVPGVLITNAI
                     QDLFGGHMLMFTTKSLEALVTAFGIGAGVGSVLILV"
     gene            876..1328
                     /locus_tag="PPY92_002110"
     CDS             876..1328
                     /locus_tag="PPY92_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDJ2626093.1"
                     /translation="MFWILNFIFSFLASMFFCVIFDAPRKLYLSCGFVGTCGWMVYTL
                     FFNGFNVHTIYSSFFGSLALGLLSHYMARKQKEPAIIFMVTGIIPLVPGGLAYDATKN
                     LVLLNFSTAINTMLEVTLIAGAIALGLLFADQISKLIVSGFVKSFKRL"
     gene            complement(1469..2953)
                     /locus_tag="PPY92_002111"
     CDS             complement(1469..2953)
                     /locus_tag="PPY92_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDJ2626094.1"
                     /translation="MEKKNKQIDRGDLKQNLSEKFVWAIAYGSCIGWGAFILPGDWIK
                     QSGPIAASIGIVIGALLMILIAVSYGALVERFPVSGGAFAFSFLSFGRYVSFFSSWFL
                     TFGYVCVVALNATAFSLLVKFLLPDVLNNGKLYTIAGWDVYITEIIIATVLLLVFMLV
                     TIRGASVSGSLQYYFCVAMVIVVLLMFFGSFFGNNFALENLQPLAEPSKGWLVSIVVI
                     VSVAPWAYVGFDNIPQTAEEFNFAPNKTFKLIVYSLLAASLTYVVMILYTGWLSTSHQ
                     SLNGQLWLTGAVTQTAFGYIGLGVLAIAIMMGIFTGLNGFLMSSSRLLFSMGRSGIMP
                     TMFSKLHSKYKTPYVAIIFLVGVSLIAPWLGRTALTWIVDMSSTGVSIAYFITCLSAA
                     KLFSYNKQSNTYAPVYKTFAIIGSFVSFIFLALLLVPGSPAALTAPSYIALLGWLIIG
                     LIFFVIRYPKLKNMDNDELSRLILNRSENEVDDMIEEPEKEKTK"
     gene            complement(3074..3442)
                     /locus_tag="PPY92_002112"
     CDS             complement(3074..3442)
                     /locus_tag="PPY92_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDJ2626095.1"
                     /translation="MKLFIILGALNAMMAVGTGAFGAHGLQGKISDHYLSVWEKATTY
                     QMYHGLALLIIGVISGTTSINVNWAGWLIFAGIIFFSGSLYILVLTQIKVLGAITPIG
                     GVLFIIGWIMLIIATFKFAG"
     gene            complement(3575..3955)
                     /locus_tag="PPY92_002113"
     CDS             complement(3575..3955)
                     /locus_tag="PPY92_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5327 family protein"
                     /protein_id="HDJ2626096.1"
                     /translation="MVNRETLIARIEQELVQAEQAQHDHDFEKHMYAIHILTSLYVST
                     SNTPHIGEQQMNRRIANHNQMPQSQITQPTHQVTAAEIEAMGGKVNTHSAHHHNKSYS
                     QPSNQQQRLATDDDIGNGESIFDF"
     gene            complement(3956..4612)
                     /locus_tag="PPY92_002114"
     CDS             complement(3956..4612)
                     /locus_tag="PPY92_002114"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDJ2626097.1"
                     /translation="MEWSQIFHDITTKHDFKAMHDFLEKEYSTAIVYPDRENIYQAFD
                     LTPFENIKVVILGQDPYHGPNQAHGLAFSVQPNAKFPPSLRNMYKELADDIGCVRQTP
                     HLQDWAREGVLLLNTVLTVRQGEANSHRDIGWETFTDEIIKAVSDYKEHVVFILWGKP
                     AQQKIKLIDTSKHCIIKSVHPSPLSAYRGFFGSKPYSKANAYLESVGKSPINWCESEA
                     "
     gene            4796..5626
                     /gene="thiD"
                     /locus_tag="PPY92_002115"
     CDS             4796..5626
                     /gene="thiD"
                     /locus_tag="PPY92_002115"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDJ2626098.1"
                     /translation="MALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTM
                     DKDTWSHDVTPLPMDVFEKQLETALSIGPDAIKTGMLGTEEIIKRAGEVYEASNAQYF
                     VVDPVMVCKGEDEVLNPGNTEAMIKYLLPKATVVTPNLFEAGQLSGLGKLNSIEDMKK
                     AATIIFDKGAQHVIIKGGKALDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAA
                     ATTAYLANGKSPKEAVISAKAFVASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV
                     "
BASE COUNT         1964 a         1078 c          973 g         1949 t
ORIGIN      
        1 ttttaaaatc aataacaact tatcaaaaag ctcatgtggt tattttatag tgtataaact
       61 ataatgagta ttaaattctt ataaacaatg gtgatgaaat ggacataaat tcagaagaat
      121 acaaacaaga ggtacttatc aaagacgttg tcatgcttgc tgctcgcata ctattagaat
      181 ctggtgcaga aggtacgcgt gtagaagata ccatgacacg tattgcaaaa aaacttggtt
      241 acagtgaaag taacagcttt gttacaaaca ctgtcatcca gtttacgtta cattcggaat
      301 cgtttcctag aatatttaga attacctctc gagatacaaa cttaataaaa atttcgcaag
      361 ctaataaaat ttcgcgtcaa attacaaata atgaaatttc tttagccgaa gcaaaaacac
      421 aacttgaaaa aatatatgtt gctaagcgtg acagcagtct tccctttaaa ggttttgctg
      481 cagcaatgat tgcaatgagt ttcttatatt tacaaggtgg tagattgatt gatgttttaa
      541 ctgcgatatt agcaggtagt ctaggatacc tagtcactga gattttagat cgcaagttac
      601 atgcacagtt tatcccagaa ttcattggtt cattagttat tgggattatc gccgttattg
      661 gacatacact tatcccaaca ggtgacttgg caactattat cattgcggca gtcatgccta
      721 ttgttcctgg tgtattaata acaaacgcaa tacaagattt atttggtgga cacatgttga
      781 tgttcacaac gaaatcatta gaagcattgg ttactgcgtt tggcatcggt gctggcgttg
      841 gtagcgtatt aattttagta taggagtatc agactatgtt ttggatctta aactttatct
      901 ttagcttttt agcttcaatg ttcttctgcg tcatttttga tgcacctaga aaattatatc
      961 tatcatgtgg tttcgttggt acatgtggat ggatggttta caccttattc ttcaacggct
     1021 ttaatgtgca cactatatac tctagtttct ttggtagttt agcattaggc ttgttaagtc
     1081 attatatggc tcgtaaacaa aaagaacctg ccatcatttt tatggtaacg ggtatcattc
     1141 cattagtacc aggtggatta gcatacgatg ctacaaaaaa tttagtctta ttaaatttca
     1201 gcacagcaat caataccatg ttagaagtaa cacttattgc aggcgccatc gcattaggtt
     1261 tattattcgc cgaccaaatt tccaaattaa ttgtttctgg gttcgtgaaa tcttttaaac
     1321 gattataaat attgttagaa atggctcagc ccaaactctt agcaattata tataaggttc
     1381 agctgcctcg tcttaaatgt caatttataa tacaaaaaat cgcacgatac gcctccgaat
     1441 gaagaaggta tattgtgcga ttcttttatt atttagtttt ttctttttca ggttcttcaa
     1501 tcatatcatc aacttcattt tcacttctat ttaaaatcaa gcgacttaat tcatcattat
     1561 ccatattttt caatttagga tatcgaatca caaagaatat taaaccgatg attaaccatc
     1621 caagcaatgc aatataagat ggtgcagtca gtgctgcagg agaacctggc actaataaca
     1681 acgctaagaa aatgaatgat acaaatgagc cgataatagc aaacgttttg taaaccggtg
     1741 catacgtatt actttgtttg ttataactga ataatttcgc tgcagacaaa catgtaataa
     1801 agtaggcaat ggatacacct gtagatgaca tatctacaat ccaagtcaat gcagttcttc
     1861 ctagccaagg tgcaattaac gacacaccta ctaggaatat gattgcgaca tatggtgttt
     1921 tgtatttact atgtaattta ctaaacattg ttggcataat acctgaacgt cccatagaaa
     1981 ataacaagcg acttgaactc atcaagaatc catttaaacc agtaaatata cccatcataa
     2041 ttgcaattgc taatacacct aatccaatat aaccaaatgc tgtttgtgta acagcacctg
     2101 ttaaccacaa ctgcccattt aaactttgat gacttgttga taaccaacca gtgtataaaa
     2161 tcatgacaac ataagttaat gatgctgcta ataaactgta cacgataagc ttaaatgtct
     2221 tgtttggtgc aaagttaaac tcttctgctg tttgtggaat attatcaaat ccaacatatg
     2281 cccatggtgc cacggataca ataaccacaa tagacactaa ccatcctttg ctaggttcag
     2341 ctaacggttg taaattttca agtgcaaaat tattaccaaa gaatgaacca aagaacatca
     2401 ataatacgac gattaccatc gccacacaga aataatattg taatgatcca gatacacttg
     2461 cgccacgaat cgttactagc atgaatacaa gtagtaatac ggtcgcaata atgatttccg
     2521 taatataaac gtcccagccc gcaatggtgt atagtttccc attatttaag acatctggca
     2581 ataagaattt aactagtaaa ctgaatgcgg tcgcatttaa agcaacgaca cagacataac
     2641 caaaagttaa aaaccatgat gagaagaaac tcacatatct gccgaaactt aagaaactaa
     2701 aggcaaacgc gccccctgat actggaaatc tctctactaa tgcgccataa ctaaccgcaa
     2761 ttaatatcat taataatgca ccaataacta taccaattga tgctgcaatc ggacctgact
     2821 gcttaatcca gtctcctggt aagatgaatg cgccccatcc gatacatgaa ccatatgcaa
     2881 tcgcccatac aaacttttca gataggtttt gttttaaatc gcctctatct atttgcttat
     2941 tctttttttc cataaataaa aactcacctc gaaggtattc tatacccaaa agatgagttt
     3001 gtaactcttt ttcttgatta tttttattaa aaatttaatg tttagttaca taggtaatct
     3061 aaagttttaa aatttaacca gcaaatttga atgtcgcaat gattaacatt atccagccaa
     3121 tgatgaacaa tacgccacca attggcgtaa tcgcacctaa aactttaatt tgagttaata
     3181 ctaaaatata taatgatcca ctaaagaaaa taataccagc aaatattaac cagccagccc
     3241 agttaacatt gattgaagtt gtaccactaa ttacacctat aattaataat gctaagccat
     3301 ggtacatttg atacgtcgtt gctttttccc atactgataa atagtgatca cttatttttc
     3361 cttgtaaacc atgcgcacca aatgcacctg ttccgacagc catcatcgcg tttaatgcac
     3421 ctaaaataat aaataatttc attgctacct ctccttatat aattcagtat taataaatgt
     3481 ttaaatccga acactcgaat aaaagtatat gcgtcttgat tattaaccaa catacttaac
     3541 aagctattaa gtaattatgt ttcattgttg ctttttaaaa atcaaatatg gattcaccat
     3601 tgccaatgtc atcatctgtc gctaatcttt gttgttggtt tgaaggttgt gaatatgact
     3661 tattatgatg atgtgctgaa tgcgtattta ctttaccacc catcgcttca atttcagcag
     3721 ctgtcacttg atgagttggc tgcgttattt gtgattgtgg catttgatta tggttagcaa
     3781 tacgacgatt catttgttgt tcaccaatat gtggtgtatt tgatgttgaa acatataaag
     3841 atgttaatat atgtatggca tacatatgtt tttcaaagtc atggtcatgc tgtgcctgct
     3901 ctgcttgtac taattcttgc tcaattcgtg ctattaaagt ttctctattc accatctacg
     3961 cctcactttc acaccaatta attggtgatt ttcctactga ctctaaatag gcattcgctt
     4021 tggaatacgg ttttgatcca aagaatcctc tatatgcaga cagtggacta ggatgcactg
     4081 attttataat acaatgttta gatgtatcga taagctttat tttttgctgt gcaggtttcc
     4141 cccacaaaat aaagacaaca tgttctttat aatcagacac tgctttaata atttcatcag
     4201 taaatgtttc ccaaccaata tcacgatgag aatttgcttc accctgtctt acggttaaaa
     4261 ctgtattcaa taacaagacg ccttctcttg cccaatcttg taaatgcggt gtttgtctaa
     4321 cgcatccaat atcatctgct aattctttat acatattacg taaagatgga gggaattttg
     4381 cgttaggttg cactgaaaat gctaatccat gtgcttggtt tggaccatga tacgggtctt
     4441 gtcctaatat aacaactttg atattttcaa acggtgttaa atcaaacgct tgatatatat
     4501 tttccctatc agggtatacg attgcagtcg aatattcttt ttctaaaaaa tcatgcatag
     4561 ctttaaagtc atgtttcgtt gttatgtcat gaaaaatttg cgaccattcc attcaatctc
     4621 acctcaggac atattttaac atatcacact tatctaaagt gaatgacatt ttcaaaagtt
     4681 acaatagtat gaatgattca tttcaacaat catcaaatat tgaatgctta atatttttca
     4741 attaggttaa aaatgatatg atgaatacaa tattattatg taggagtgaa gctcaatggc
     4801 tttaaagaaa gttttaacaa ttgccggttc tgacacaagt gctggcgcag gtatgcaagc
     4861 agatttgaaa acgttccaag aattagatac gtatggcatg gtcgctttaa ctgccatcgt
     4921 tactatggat aaagatacat ggtcacacga tgttacacca ttaccaatgg atgtatttga
     4981 aaaacaactt gaaacggctt tatcaatcgg acctgatgct attaaaacag gtatgttagg
     5041 tactgaggag ataatcaaac gtgctggaga agtatatgag gcatctaatg cacaatattt
     5101 tgtagtagat cctgtcatgg tttgtaaagg cgaagatgaa gtgcttaatc ctggaaatac
     5161 tgaggcgatg attaaatatt tactacctaa agcaacggta gtgacaccaa acttatttga
     5221 agcgggtcaa ttatcaggat taggtaaatt aaattcaatt gaagatatga aaaaggctgc
     5281 aacaattatc tttgataaag gcgcacagca tgtcatcatt aaaggtggca aagctttaga
     5341 tcaagataaa tcgtatgact tatactatga tggtcaaaca ttttatcaac taacaacaga
     5401 catgttccaa caaagttata accatggtgc tggttgtaca tttgctgcgg caacaactgc
     5461 atatttagct aacggtaagt caccgaaaga agctgtgatt agcgcgaaag ctttcgtcgc
     5521 ttctgctatt aaaaacggtt ggaaaatgaa tgattttgtt ggtcctgtgg atcacggtgc
     5581 atacaaccgt attgaacata tcgatgttga agtaacagag gtttaacgat taggcggttc
     5641 atctataaat caacattatt agccacacca cttaaattgg gtatgaactc aatttatgtg
     5701 atgtggttat ttttttataa tcatcatgct ctgtatataa tttaaatttg aaagttacat
     5761 ataagcgtta ttagctttta ttgaaaattt catacatatc gaacaataca tacaaatatt
     5821 tcctatacat ctacatctat gactaaataa ccctttattt tcaagatgaa cttaaactca
     5881 tctatatgct caatacctct atcattatca ttcttttgcg atagatgcct ctctaatcat
     5941 atatgttata tttcccaaat gact
//
LOCUS       DANBWH010000161         5898 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.167, whole genome shotgun sequence.
ACCESSION   DANBWH010000161 DANBWH010000000
VERSION     DANBWH010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5898
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.167"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            65..1024
                     /locus_tag="PPY92_002116"
     CDS             65..1024
                     /locus_tag="PPY92_002116"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDJ2626099.1"
                     /translation="MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVK
                     ADVQDLNHGTVHSPSPVDVKAGEYEDCKDADLVVITAGAPQKPGETRLQLVEKNTKIM
                     KSIVKSVMDSGFDGYFLIAANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLIS
                     QELGVAPSSVDASIIGEHGDTELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTR
                     DAAYEIIQAKGSTYYGIALALMRISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPT
                     LVNQHGAVKIYEMPLSAEEQALFDKSVKILEDTFDSIKYLLED"
     gene            1302..2006
                     /gene="budA"
                     /locus_tag="PPY92_002117"
     CDS             1302..2006
                     /gene="budA"
                     /locus_tag="PPY92_002117"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDJ2626100.1"
                     /translation="MTNVLYQHGTLGTLMAGLLEGTATINELLEHGNLGIATLTGSDG
                     EVIFLDGKAYHANEHKEFIELKGDEKVPYASITNFKASKTFPLQQLSQDDVFAQIKNE
                     MLSENLFSAVKIYGTFKHMHVRMMPAQQPPYTRLIDSARRQPEEKRQDIRGAIVGFFT
                     PELFHGVGSAGFHIHFADDERAYGGHVLDFEVDDVVVEIQNFETFQQHFPVNNETFVK
                     AKIDYKDVAEEIREAE"
     gene            complement(2092..2952)
                     /locus_tag="PPY92_002118"
     CDS             complement(2092..2952)
                     /locus_tag="PPY92_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDJ2626101.1"
                     /translation="MLSVAIIGPGAVGTTIAYELQQSLPHTTLIGRHAKTITYYTVPH
                     APAQDIVVKGYEDVTNTFDVIIIAVKTHQLDAVIPHLTYLAHEDTLIILAQNGYGQLE
                     HIPFKNVCQAVVYISGQKKGDVVTHFRDYQLRIQDNALTRQFRDLVQDSQIDIVLEAN
                     IQQAIWYKLLVNLGINSITALGRQTVAIMHNPEIRILCRQLLLDGCRVAQAEGLNFSE
                     QTVDTIMTIYQGYPDEMGTSMYYDIVHQQPLEVEAIQGFIYRRAREHNLDTPYLDTIY
                     SFLRAYQQNM"
     gene            3025..3843
                     /gene="panB"
                     /locus_tag="PPY92_002119"
     CDS             3025..3843
                     /gene="panB"
                     /locus_tag="PPY92_002119"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDJ2626102.1"
                     /translation="MKTVSQLIDMKQKQTKISMVTAYDFPSAKQVEAAGIDMILVGDS
                     LGMTVLGYESTVQVTLADMIHHGRAVRRGAPNTFVVVDMPIGAVGISMTQDLNHALKL
                     YQETNANAIKAEGAHITPFIEKATAIGIPVVAHLGLTPQSVGVMGYKLQGATKEAAEQ
                     LILDAKNVEQAGAVALVLEAIPNDLAEEISKHLTIPVIGIGAGKGTDGQVLVYHDMLN
                     YGVEHKAKFVKQFADFSVGVDGLKQYDQEVKSGAFPSEEYTYKKKIMNEVNNND"
     gene            3836..4687
                     /locus_tag="PPY92_002120"
     CDS             3836..4687
                     /locus_tag="PPY92_002120"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDJ2626103.1"
                     /translation="MTKLITTVKEMQHIVKAAKRSGTTIGFIPTMGALHDGHLTMVRE
                     SVSTNDITVVSVFVNPLQFGPNEDFDAYPRQIDKDLELVSEVGADIVFHPAVEDIYPG
                     ELGIDVKVGPLADVLEGAKRPGHFDGVVTVVNKLFNIVMPDYAYFGKKDAQQLAIVEQ
                     MVKDFNHAVEIIGIDIVREADGLAKSSRNVYLTEQERQEAVHLSKSLLLAQALYQDGE
                     RQSKVIIDRVTEYLESHISGRIEEVAVYSYPQLVEQHEITGRIFISLAVKFSKARLID
                     NIIIGAE"
     gene            4689..5072
                     /locus_tag="PPY92_002121"
     CDS             4689..5072
                     /locus_tag="PPY92_002121"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015901315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HDJ2626104.1"
                     /translation="MIRTMMNAKIHRARVTESNLNYVGSITIDSDILEAVDILPNEKV
                     AIVNNNNGARFETYVIAGERGSGKICLNGAASRLVEVGDVVIIMTYAQLNEEEIKNHA
                     PKVAVMNEDNVIIEMIHEKENTIVL"
     gene            5415..>5898
                     /locus_tag="PPY92_002122"
     CDS             5415..>5898
                     /locus_tag="PPY92_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HDJ2626105.1"
                     /translation="MTKIFKISFLILLVVIVSSYYGESFVLADNNPIVNSENGSDNNF
                     ESGTIERNQNELYEVGDYSTPLTERVFGKDQRTVVNNILQRPYKQTVLLNMTFSNNRV
                     YKGTGTMIGKDIVLTAAHNVYSKDDKGWAKKIDVYAGVNGQTYTIGKAFSHKFFVSKT
                     W"
BASE COUNT         2040 a          805 c         1233 g         1820 t
ORIGIN      
        1 agtatagata tttttacgtg tagtcacgtg taaaataaat tcaattaggt taggagacat
       61 aattatgaaa acatttggta aaaaggttgt attaatcgga gatggatctg taggatcaag
      121 ctatgccttt gcaatggtta cgcaaggtgt tgctgatgaa tttgtaatta ttgacattgc
      181 aaaagacaaa gtaaaagcag atgttcaaga tttaaaccat ggtacagtcc acagtccttc
      241 accagttgat gtgaaagcag gtgaatacga agactgtaaa gatgcagatt tagttgttat
      301 tacagctggt gcacctcaaa agccaggtga aacacgttta caattagttg aaaaaaatac
      361 taagattatg aagagtattg ttaagagtgt tatggatagc ggctttgatg gatatttctt
      421 aatcgcggca aaccctgttg acattttaac aagatttgta aaagaatata ctggtttacc
      481 agcagagcgt gttatcggtt caggtactgt attggacagt gcacgtttac aatatttaat
      541 tagccaagaa cttggtgttg caccttcaag tgttgacgct agtattattg gtgagcatgg
      601 tgatactgaa cttgcagttt ggtcacaagc aaatgtagca ggtatttcag tatatgacac
      661 attaaaagaa caaactggta gcgaagctaa agcggaagaa atttatgtaa atacacgtga
      721 cgctgcttat gaaattatcc aagctaaagg gtcaacatac tatggaattg cattagcatt
      781 gatgcgcatt tcaaaagcca ttttaaataa tgaaaataat gtcttaaatg tttctataca
      841 attagatggt caatatggtg gtcacaaagg cgtttaccta ggtgtaccaa cattagttaa
      901 ccaacatggc gcagttaaaa tttatgaaat gccattaagt gccgaagaac aagcgttgtt
      961 cgataaatct gttaaaatat tagaagatac atttgattca attaaatatt tattagaaga
     1021 ctaagcctat tttaagtatt aattagaaat atattaatgg taagaggatc tattagtgtt
     1081 gcagtaacat gtggcactga tagatccatt tttttagcaa gcattgatag aggcaatact
     1141 tttgcgtaaa ataataattg aataaagtat tatgactcgt gaaaatttag tgacatgaat
     1201 cgaaatgccg aggcgtcatt gagtataaag gaaagtagtt ctgcattaag cacgaagtag
     1261 agcatgacaa cgaggaataa ctatagggag atggttttgg aatgacgaat gtcttgtatc
     1321 aacatggtac attaggtacg ttaatggctg gcttactaga aggaacagct acaattaatg
     1381 aattgttaga acatgggaat ttagggattg caacgttaac agggtctgat ggcgaagtaa
     1441 tatttttaga cgggaaggca tatcatgcta acgagcataa agaatttata gaattaaaag
     1501 gcgatgagaa agtaccgtat gcatcgatta ctaattttaa agcgagtaag acatttccat
     1561 tgcaacaatt atcacaagat gatgtattcg cacaaattaa aaatgaaatg ttaagtgaga
     1621 atttattttc ggctgttaaa atttatggca catttaaaca tatgcatgta cgaatgatgc
     1681 ctgctcagca accgccatat acacgtttga ttgattcagc acgcagacaa cctgaggaaa
     1741 aaagacaaga tattcgtggt gccattgttg gattttttac accagaatta tttcatggcg
     1801 tagggtctgc tggttttcat atacattttg cggatgatga aagagcttat ggtggacatg
     1861 ttcttgactt tgaagtggat gacgttgtcg ttgagataca aaactttgaa acattccaac
     1921 aacatttccc ggtaaataac gagacgtttg ttaaagcgaa aatagactat aaagatgtgg
     1981 cagaagaaat tagagaagct gaatagtcta atataattaa aagaccttag cgatattagg
     2041 aacaggtggt tctaaatgca tcgttaaggt ctttttatat tatatatgtg cttacatatt
     2101 ttgttgataa gcgcgtaaaa agctataaat agtatcaaga tatggggtgt caagattgtg
     2161 ttcacgtgca cgtcgataaa taaagccttg aatcgcttcg acttcaagag gttgctgatg
     2221 cacaatgtcg taatacatgc tcgttcccat ttcgtcggga taaccttgat atatagtcat
     2281 aatagtatcg acagtttgtt ctgaaaagtt taacccctct gcctgtgcaa cacgacaacc
     2341 atctagtaat aactgtctac acagtatacg tatttcagga ttatgcatga ttgcaactgt
     2401 ctgtctcccg agtgctgtga tagaattaat gcccaagtta actaacaatt tataccaaat
     2461 agcttgttga atatttgctt ctaatacaat gtcgatttga ctgtcttgga ccaaatctct
     2521 aaattgtcga gttaatgcat tatcttgtat acgtaattga taatctctga agtgcgtaac
     2581 aacatcgcct ttcttttgac cacttatata gacaactgct tggcatacat ttttaaatgg
     2641 aatatgttcg agttgaccat aaccattttg ggctaaaatg atgagcgtgt cctcatgtgc
     2701 aagataagtt aaatgaggaa taacggcatc aagttgatgt gttttgactg caataataat
     2761 aacatcaaat gtgtttgtga catcttcata acctttcaca acaatatctt gtgcaggtgc
     2821 atgtggtaca gtataatatg ttattgtttt ggcgtgtctc ccgataagcg ttgtatgagg
     2881 caatgattgt tgtaattcat aggcaatagt tgtaccgaca gcgcctgggc cgataatcgc
     2941 aactgataac atcttcttct ttcctttcta taaaagattc attatactat ttctaataat
     3001 actgcaaaaa gaggttgaat aacattgaaa acagtaagtc aattaataga tatgaaacaa
     3061 aagcaaacta aaatttctat ggtaacagct tatgattttc caagtgctaa acaagtggaa
     3121 gcagcgggta ttgatatgat tcttgttggg gattcacttg gtatgactgt attaggttat
     3181 gaaagtaccg tacaagtgac gttagcagat atgattcatc acggtcgtgc agtaagaaga
     3241 ggcgcaccaa atacatttgt agttgttgat atgccgattg gtgcagtagg catctctatg
     3301 acacaagact taaatcatgc tctaaagctt tatcaggaga cgaatgcaaa tgcaattaaa
     3361 gcagaaggag cgcatattac accatttatt gaaaaagcaa cggcaattgg tattcctgtt
     3421 gttgcacatt taggattaac acctcaaagt gttggcgtta tggggtataa attacaaggt
     3481 gcaacgaaag aagcagcgga gcaacttatt ttagatgcga aaaatgtaga acaagctggt
     3541 gcagttgcgt tagtacttga agcaattcca aatgatttag cagaggaaat tagcaagcac
     3601 ttaacaattc cagtcattgg tattggtgca ggaaaaggta cagatggtca agtattggtt
     3661 tatcacgata tgttaaatta tggtgttgaa cataaagcga aatttgtgaa gcaatttgct
     3721 gattttagtg ttggtgtgga tggtttaaaa caatacgatc aagaagttaa atctggtgca
     3781 tttccatcag aagaatatac gtataaaaag aaaattatga atgaggttaa taacaatgac
     3841 taagctgatt actacggtaa aagagatgca acacattgtt aaagcagcca aacgctcagg
     3901 aacaacgatt ggttttatcc caacaatggg tgccttacat gatggacatt taacgatggt
     3961 acgcgaatca gttagtacaa atgatattac agttgtgagt gtatttgtta atccattaca
     4021 atttggtcca aacgaagatt tcgatgctta tccgagacaa attgataaag atttagaatt
     4081 agtatcagaa gtaggggcgg acattgtttt tcatcctgca gtagaagata tatatccagg
     4141 tgaattaggt attgatgtca aagtaggtcc attagctgat gtgttagaag gagcgaagcg
     4201 tccagggcat tttgatgggg tggtaacagt agttaataag ctatttaata ttgttatgcc
     4261 ggattatgct tattttggta aaaaagatgc ccagcaattg gctattgtcg agcaaatggt
     4321 aaaagacttc aatcatgccg ttgaaattat tggtattgat atcgttcgag aagcagatgg
     4381 tttggcgaaa agttcaagaa atgtttattt aacggagcaa gaacgacaag aagcggtaca
     4441 tttaagtaaa agtttgctat tagcacaagc gttgtatcaa gacggtgaac gtcaaagtaa
     4501 agtaattata gacagggtta ctgaatatct tgaatcacat ataagtggac gcattgaaga
     4561 agttgctgtt tatagttatc ctcaattagt agaacaacac gaaataactg gacgcatatt
     4621 tatctcgtta gctgtcaaat tttcaaaagc gcgtttaata gataatataa taattggagc
     4681 tgaataaagt gataagaaca atgatgaatg ctaaaattca tagagcaaga gtgactgagt
     4741 caaatttaaa ttatgtaggt agtattacga ttgattcaga tattttggaa gcggtagaca
     4801 tcttgccaaa tgaaaaagta gccatcgtga ataataataa tggtgcacgt tttgaaacat
     4861 acgtaatagc tggtgaaaga ggtagtggta aaatttgttt gaacggtgca gcatcaagac
     4921 ttgttgaagt aggcgatgta gtcataatca tgacttatgc acagttaaat gaagaagaaa
     4981 ttaaaaacca tgctcctaaa gtagcagtaa tgaatgaaga caatgtcatt attgaaatga
     5041 ttcatgaaaa agaaaatacg atagtgttat aaaatagtat aagttagaaa cctctagtag
     5101 ataacaatgt agttatccat taggggtttt tatttttatg ccaattgggt tagcgtaata
     5161 gaagtaaagg tattatcgtt gcaattttat cagtgttaaa tgatggacat atttataaag
     5221 aaatagtttt gcgaattgat tatctaaaac agtattcaga atttatttgg tagaaaatag
     5281 aattcttaac ttaacgagat aagttaatga catgttgttt agtttcgtga agtaaaaata
     5341 ttgaattgat agcaattata taataaaatt atatgaaata gataaaaata atatagatta
     5401 aaggaggaat gcctatgaca aaaattttta aaatatcatt tttgattttg ttagtagtaa
     5461 ttgtttctag ttattatggt gaaagttttg tcttagctga taataatcct atcgtcaatt
     5521 cagaaaatgg tagtgataat aattttgaat caggaacaat tgaacgtaat caaaatgagc
     5581 tttatgaagt aggtgattat agtacgccct taactgagcg agttttcggt aaagatcaaa
     5641 gaacggttgt taataatatt ttacaaagac cttataagca gactgtatta ttaaatatga
     5701 cgttttcaaa taaccgtgta tataaaggca ccggtactat gattggtaag gacattgtat
     5761 tgacagcggc tcataatgtc tattctaaag acgataaggg ttgggctaaa aagattgatg
     5821 tctatgctgg agtaaatggg caaacatata caataggaaa ggccttttcg cataagtttt
     5881 ttgtatctaa aacttgga
//
LOCUS       DANBWH010000162         5895 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.168, whole genome shotgun sequence.
ACCESSION   DANBWH010000162 DANBWH010000000
VERSION     DANBWH010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5895
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.168"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            256..1176
                     /gene="mvk"
                     /locus_tag="PPY92_002123"
     CDS             256..1176
                     /gene="mvk"
                     /locus_tag="PPY92_002123"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HDJ2626106.1"
                     /translation="MTRKGYGESTGKIILIGEHAVTFGEPAIAVPFNAGKIKVLIEAL
                     ESGNYSSIKSDVYDGMLYDAPDHLKSLVNRFVELNNITEPLAVTIQTNLPPSRGLGSS
                     AAVAVAFVRASYDFLGKSLTKEELIEKANWAEQIAHGKPSGIDTQTIVSGKPVWFQKG
                     HAETLKTLSLDGYMVVIDTGVKGSTRQAVEDVHKLCEDPQYMSHVKHIGKLVLRASDV
                     IEHHNFEALADIFNECHADLKALTVSHDKIEQLMKIGKENGAIAGKLTGAGRGGSMLL
                     LAKDLPTAKNIVKAVEKAGAAHTWIENLGG"
     gene            1181..2164
                     /gene="mvaD"
                     /locus_tag="PPY92_002124"
     CDS             1181..2164
                     /gene="mvaD"
                     /locus_tag="PPY92_002124"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HDJ2626107.1"
                     /translation="MIKSGKARAHTNIALIKYWGKKDEALIIPMNNSISVTLEKFYTE
                     TKVTFNDQLTQDQFWLNGEKVSGKELEKISKYMDIVRNRAGIDWYAEIESDNFVPTAA
                     GLASSASAYAALAAACNQALDLQLSDKDLSRLARIGSGSASRSIYGGFAEWEKGYNDE
                     TSYAVPLESNHFEDDLAMIFVVINQHSKKVPSRYGMSLTRNTSRFYQYWLDHIDEDLA
                     EAKAAIQDKDFKRLGEVIEENGLRMHATNLGSTPPFTYLVQESYDVMALVHECREAGY
                     PCYFTMDAGPNVKILVEKKNKQQIIDKLLTQFDNNQIIDSDIIATGIEIIE"
     gene            2177..3253
                     /locus_tag="PPY92_002125"
     CDS             2177..3253
                     /locus_tag="PPY92_002125"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HDJ2626108.1"
                     /translation="MIQVKAPGKLYIAGEYAVTEPGYKSVLIALDRFVTATIEEADQY
                     KGTIHSKALHHNPVTFSRDEDSIVISDPHAAKQLNYVVTAIEIFEQYAKSCDIAMKHF
                     HLTIDSNLDDSNGHKYGLGSSAAVLVSVIKVLNEFYDMKLSNLYIYKLAVIANMKLQS
                     LSSCGDIAVSVYSGWLAYSTFDHEWVKHQIEDTTVEEVLIKNWPGLHIEPLQAPENME
                     VLIGWTGSPASSPHFVSEVKRLKSDPSFYGDFLEDSHRCVEKLIHAFKTNNIKGVQKM
                     VRQNRTIIQRMDKEATVDIETEKLKYLCDIAEKYHGASKTSGAGGGDCGITIINKDVD
                     KEKIYDEWTKHGIKPLKFNIYHGQ"
     gene            3430..3771
                     /locus_tag="PPY92_002126"
     CDS             3430..3771
                     /locus_tag="PPY92_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HDJ2626109.1"
                     /translation="MKNTFLICDECQAVNIRTLQKKLEKLDPDAEIVIGCQSYCGPGR
                     RKTFTFVNNRPLAALTEEELIEKVSQQLKKPRDPEEEERLRKRHEERKRRKEEQDRKL
                     KEKLEKRKAQQ"
     gene            complement(3823..5145)
                     /locus_tag="PPY92_002127"
     CDS             complement(3823..5145)
                     /locus_tag="PPY92_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HDJ2626110.1"
                     /translation="MKTYDLIVIGFGKAGKTLAKYAASTGQHVAVIEQSSKMYGGTCI
                     NIGCIPSKTLVHDGLEGKSFEASYNRKNDVVNALNNKNYHLLADDNNIDVLDFKAQFK
                     SNTEVNLLDQHGDIVDSITAPHIIINTGATSIIPNIKGLDQAKHVFDSTGLLNISYQP
                     KHLVIVGGGYIALEFASMFANLGSKVTVLERGESFMPREDQDVVAHAITDLENKGIAL
                     HTNVETTELSSDDHHTTVRTNVGNFEADAVLLAIGRKPNTALALENTDIELGDRGEIK
                     VNAQLQTSVPHIYAAGDVKGGLQFTYISLDDYRIIKSALYGNQSRTTDNRGSVPYTVF
                     IDPPLSRVGLTSKEAAAQHYDYTEHQLLVSAIPRHKINNDPRGLFKVVINNENNMILG
                     ATLYGKQSEELINIIKLAIDQNIPYTVLRDNIYTHPTMAESFNDLFNF"
     gene            complement(5132..5572)
                     /locus_tag="PPY92_002128"
     CDS             complement(5132..5572)
                     /locus_tag="PPY92_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDJ2626111.1"
                     /translation="MNLEFNIAVHVLAFLTKHHSEKFNSSSLAELTCLNPVQLRRVTT
                     QLVDLKMIDTIRGKDGGYLANDQSADVSLATLYKHFVLEKEHHTRLFTGDEGSHCQIA
                     RNIATTMSHYQQDEQNIIINFYNEKTIKDVIEDIQKEDLCHENI"
     gene            5630..5731
                     /locus_tag="PPY92_002129"
     CDS             5630..5731
                     /locus_tag="PPY92_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626112.1"
                     /translation="MCQYLCLKSVELGVVIVMIYCLKVDALTVRLHN"
BASE COUNT         2043 a          865 c         1153 g         1834 t
ORIGIN      
        1 ttataaaaag ataaagaatc tgaaatatca ctcgataaga tttaaacttt gaagtaaacg
       61 aaagttagtt ttatactaat caagttaagt caaatataaa ttcaaaataa agattataca
      121 taatatgaat aatgtgagat aaagactgca atgaaattta acgcgaatgc gtatgcaact
      181 tagaatatga tgtcttttac taataaataa aaataatgaa tcaagtatag gataaatata
      241 aaaggtgata ttgacatgac aagaaaagga tatggggaat cgacaggtaa gattatttta
      301 ataggagaac atgctgttac atttggagag cctgctattg cagtaccgtt taacgcaggt
      361 aaaatcaaag ttttaataga agccttagag agcgggaact attcgtctat taaaagcgat
      421 gtttacgatg gtatgttata tgatgcgcct gaccatctta agtctttggt gaaccgtttt
      481 gtagaattaa ataatattac agagccgcta gcagtaacga tccaaacgaa tttaccacca
      541 tcacgtggat taggatcgag tgcagctgtc gcggttgctt ttgttcgtgc aagttatgat
      601 tttttaggga aatcattaac gaaagaagaa ctcattgaaa aggctaattg ggcagagcaa
      661 attgcacatg gtaaaccaag tggtattgat acgcaaacga ttgtatcagg caaaccagtt
      721 tggttccaaa aaggtcatgc tgaaacattg aaaacgttaa gtttagacgg ctatatggtt
      781 gttattgata ctggtgtgaa aggttcaaca agacaagcgg tagaagatgt tcataaactt
      841 tgtgaggatc ctcagtacat gtcacatgta aaacatatcg gtaagttagt tttacgtgcg
      901 agtgatgtga ttgaacatca taactttgaa gccctagcgg atatttttaa tgaatgtcat
      961 gcggatttaa aggcgttgac agttagtcat gataaaatag aacaattaat gaaaattggt
     1021 aaagaaaatg gtgcgattgc tggaaaactt actggtgctg gtcgtggtgg aagtatgtta
     1081 ttgcttgcca aagatttacc aacagcgaaa aatattgtga aagctgtaga aaaagctggt
     1141 gcagcacata catggattga gaatttagga ggttaatgcg ttgattaaaa gtggcaaagc
     1201 acgtgcacat acgaatattg cacttataaa atattggggt aaaaaagatg aagcactaat
     1261 cattccaatg aataatagca tatctgttac attagaaaaa ttttacactg aaacgaaagt
     1321 cacttttaac gaccagttaa cacaggatca attttggttg aatggtgaaa aggttagtgg
     1381 caaagaatta gagaaaattt caaaatatat ggatattgtc agaaatagag ctggcatcga
     1441 ttggtatgct gaaattgaaa gcgacaattt tgtaccaaca gcagcagggt tggcttcatc
     1501 agcaagcgca tatgcagctt tagcagcagc ttgtaatcaa gcactagact tgcagctgtc
     1561 agataaggat ttatcgagat tggcgcgaat tggttcgggt tctgcgtcgc gtagtattta
     1621 tggtggattt gcagaatggg aaaaagggta taatgatgag acgtcatatg ccgttccact
     1681 tgaatcgaat cattttgaag atgaccttgc catgatattt gttgtgatta atcaacattc
     1741 taaaaaggta cctagtcgat atggtatgtc gttgacacga aacacatcaa ggttttatca
     1801 atattggtta gatcatattg atgaagattt agctgaagca aaagcagcga ttcaagacaa
     1861 agattttaaa cgccttggtg aagtaattga agaaaatggt ttacgtatgc atgccacgaa
     1921 tctgggatca acaccgccgt tcacttatct tgtgcaagaa agttatgatg tcatggcgct
     1981 cgttcacgaa tgccgagaag cgggatatcc gtgttatttt acgatggatg cgggtcctaa
     2041 tgtgaaaata cttgtagaaa agaaaaacaa gcaacagatt atagataaat tattaacaca
     2101 gtttgataat aaccaaatta ttgatagtga cattattgcc acaggaattg aaataattga
     2161 gtaaggaaga gataaaatga ttcaggtcaa agcacccgga aaactttata ttgctggaga
     2221 atatgctgta acagaaccag gatataaatc tgtacttatt gcgttagatc gttttgtaac
     2281 tgctactatt gaagaagcag accaatataa aggtaccatt cattcaaaag cattacatca
     2341 taacccagtt acatttagta gagatgaaga tagtattgtc atttcagatc cacatgcagc
     2401 aaaacaatta aattatgtgg tcacagctat tgaaatattt gaacaatacg cgaaaagttg
     2461 cgatatagcg atgaagcatt ttcatctgac tattgatagt aatttagatg attcaaatgg
     2521 tcataaatat ggattaggtt caagtgcagc agtacttgtg tcagttataa aagtattaaa
     2581 tgaattttat gatatgaagt tatctaattt atacatttat aaactagcag tgattgcaaa
     2641 tatgaagtta caaagtttaa gttcatgcgg agatattgct gtgagtgtat atagtggatg
     2701 gttagcgtat agtacttttg atcatgaatg ggttaagcat caaattgaag atactacggt
     2761 tgaagaagtt ttaatcaaaa actggcctgg attgcacatc gaaccattac aagcacctga
     2821 aaatatggaa gtacttatcg gttggactgg ctcaccggcg tcatcaccac actttgttag
     2881 cgaagtgaaa cgtttgaaat cagatccttc attttacggt gacttcttag aagattcaca
     2941 tcgttgtgtt gaaaagctta ttcatgcttt taaaacaaat aacattaaag gtgtgcaaaa
     3001 gatggtgcgt cagaatcgta caattattca acgtatggat aaagaagcta cagttgatat
     3061 agaaactgaa aagctaaaat atttgtgtga tattgctgaa aagtatcacg gtgcatctaa
     3121 aacatcaggc gctggtggtg gagactgtgg tattacaatt atcaataaag atgtagataa
     3181 agaaaaaatt tatgatgaat ggacaaaaca tggtattaaa ccattaaaat ttaatattta
     3241 tcatgggcaa taaatgattt gataggggag caatcatcta atttaattag aagataaatg
     3301 ctccattttt tattgccagc cgtgttacgt atcttgaaaa aaatattaaa aagagtaaaa
     3361 tagatagagt taacgaaaaa attaatgaaa tcgacgatat agatatgata aagaaaggtg
     3421 ggtagcaata tgaaaaatac attccttatt tgtgatgaat gtcaggcagt caatataaga
     3481 acgttacaaa agaagttgga aaaattagat cccgatgctg aaatcgtgat aggttgtcaa
     3541 tcttattgtg gacctggacg ccgaaaaaca ttcacttttg ttaataaccg cccactggct
     3601 gcgcttactg aagaagaatt aatcgaaaaa gtttctcaac aattaaagaa accacgtgat
     3661 cctgaagaag aagagcgttt aagaaaacga catgaagaac gtaaacgtcg taaagaagaa
     3721 caagatagaa agcttaaaga aaaattagaa aagcgaaaag cacaacaata aaacctgatg
     3781 gcagcatcat tcaatgcgtg ccaccaggtt tttatgtttt gtctagaaat taaataaatc
     3841 attaaatgat tcggccatcg taggatgtgt ataaatatta tctcgtaata cggtatatgg
     3901 aatgttttga tcaatcgcaa gtttaattat attaattaat tcttcagatt gcttaccata
     3961 taatgtagca cctaaaatca tattattttc attattaatg actactttaa ataaacctct
     4021 tggatcattg ttaattttgt gacgaggtat agcacttact aaaagttgat gttcagtgta
     4081 atcataatgt tgagcggcag cttctttact agttaatcca acacgtgata atggtggatc
     4141 tataaatact gtataaggca cgctacctct attgtcagtc gtacgtgact gattaccata
     4201 taacgctgat ttgataattc gataatcatc taaagatata tacgtaaatt gaagtccgcc
     4261 tttaacatca cctgcagcat aaatatgtgg cacagatgtt tgaagttgag cattgacttt
     4321 aatttcgcct ctatcaccta attcgatatc agtattttct aaagctaagg ctgtattcgg
     4381 tttgcgcccg atagccaaaa gtactgcatc agcctcaaag ttgccaacat tagtacggac
     4441 tgttgtatga tgatcgtcag atgacaattc agtcgtttca acatttgtat gcaatgcaat
     4501 gcctttattt tctaagtcag taatagcatg tgcaacgaca tcttgatctt cgcgtggcat
     4561 aaatgattca ccacgttcta atactgttac cttactacct aaattcgcaa acattgaagc
     4621 aaattctaag gcaatataac cgccacctac aataacgaga tgcttaggtt gatagctaat
     4681 gtttaataaa cctgttgaat cgaagacatg tttagcttga tcaaggcctt ttatgttagg
     4741 aatgatagag gtagcaccgg tattaataat gatatgaggt gcagtaatac tatcgacgat
     4801 atcgccatgc tgatctaata aattcacttc agtattagat ttaaactgcg ctttaaaatc
     4861 caatacatca atgttgttat cgtctgctaa taagtggtaa tttttattgt ttagcgcatt
     4921 gacaacatcg tttttacggt tataacttgc ttcaaaagat ttgccttcta atccatcatg
     4981 tacaagtgtc ttcgaaggta tacatcctat atttatacaa gtgccgccat acattttcga
     5041 agattgttcg ataactgcga cgtgttgacc tgttgatgca gcatatttcg ctaaagtttt
     5101 accagctttc ccaaatccta ttacaattaa atcatatgtt ttcatgacat aaatcctcct
     5161 tttgaatgtc ttcaatgaca tctttgattg ttttttcatt ataaaaatta ataatgatat
     5221 tctgttcgtc ttgctgataa tgtgacatgg tagttgcaat attacgagca atttgacagt
     5281 gactgccttc gtcgccagta aatagacgtg tgtggtgttc tttctctaag acaaaatgtt
     5341 tatataatgt tgctagagag acatcagcac tttgatcatt tgctaaataa ccgccatctt
     5401 tacctcgtat tgtgtcaatc atttttaaat cgacaagttg agtcgtcacg cgtcgtaatt
     5461 gaacaggatt taaacaagtt aattctgcta atgaactact attgaatttt tctgaatgat
     5521 gcttagttaa aaaagctaat acatgcacgg caatgttaaa ttctaaattc aatgtttctc
     5581 acctcataat tgtaactgta ttagttacaa ttaaaaatca ttttgctcaa tgtgtcaata
     5641 cttatgtttg aaaagtgttg aattaggtgt ggttattgtg atgatttatt gtttgaaagt
     5701 tgatgcatta acggtgagat tgcacaattg aaattttaat tatgtatgaa ctgatagata
     5761 tgaaatgaat attgaaaatg agagcaataa aattttgaat gatgatgttg tgtgattgta
     5821 taaacttatt cgtattttag atacgttttg atatttcgca tgatgaggaa atacttttaa
     5881 caactgatga catat
//
LOCUS       DANBWH010000163         5848 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.169, whole genome shotgun sequence.
ACCESSION   DANBWH010000163 DANBWH010000000
VERSION     DANBWH010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5848
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.169"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            45..1100
                     /locus_tag="PPY92_002130"
     CDS             45..1100
                     /locus_tag="PPY92_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HDJ2626113.1"
                     /translation="MVTLLLVAVTMIVSLTITPIVIAISKRLNLVDKPNFRKVHTKPI
                     SVMGGTVILFSFLIGIWIGHPIETEIKPLIIGAIIMYVLGLVDDIYDLKPYIKLAGQI
                     AAALVVAFYGVTIDFISLPMGTTIHFGFLSIPITVIWIVAITNAINLIDGLDGLASGV
                     SAIGLITIGFIAILQANIFITMICCVLLGSLIGFLFYNFHPAKIFLGDSGALMIGFII
                     GFLSLLGFKNITIIALFFPIVILAVPFIDTLFAMIRRVKKGQHIMQADKSHLHHKLLA
                     LGYTHRQTVLLIYSISILFSLSSIILYVSPPLGVVLMFVLIIFSIELIVEFTGLIDNN
                     YRPILNLISRKSSHKED"
     gene            complement(1265..1906)
                     /locus_tag="PPY92_002131"
     CDS             complement(1265..1906)
                     /locus_tag="PPY92_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDJ2626114.1"
                     /translation="MTQNIITVKKEHIIENVISKSRFIAHIKPVQNEDEAKAFIAAIK
                     KEHKDATHNCSAYTIGPEMNIQKANDDGEPTGTAGVPMLDILKKLDVHNACVVVTRYF
                     GGIKLGGGGLIRAYSGAVRDVIYDVGRVELREAVPCIVTLNYDQTGKFEYELASTHFM
                     LRNQTYTDKVSYYIDVVKSDYEAFINFLNQMTAGNFDLTEETPKQLPFDIPTE"
     gene            2050..2916
                     /gene="fakB1"
                     /locus_tag="PPY92_002132"
     CDS             2050..2916
                     /gene="fakB1"
                     /locus_tag="PPY92_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB1"
                     /protein_id="HDJ2626115.1"
                     /translation="MKIAVMTDSTSYLSQDLIDKYNIQIAPLSVTFDDGKNFTESNEI
                     AIEEFYNKMASSQTIPTTSQPAIGEWITKYEMLRDQGYTDIIVICLSSGISGSYQSSY
                     QAGEMVEGVNVHAFDSKLAAMIEGCYVLRAIEMVEEGYEPQQIIDDLTNMRERTGAYL
                     IVDDLKNLQKSGRITGAQAWVGTLLKMKPVLKFEDGKIIPEEKVRTKKRAIQTLEKKV
                     LDIVKDFEEVTLFVINGDHFEDGQALYKKLQEDCPSGYQVAYSEFGPVVAAHLGSGGL
                     GLGYVGRKIRLT"
     gene            3280..4362
                     /locus_tag="PPY92_002133"
     CDS             3280..4362
                     /locus_tag="PPY92_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDJ2626116.1"
                     /translation="MDNVTRYKITESSQSSSQAYYHLSFELSEQQSYASEHIVRAIRM
                     RQTILLYAVTGAGKTEMMFQGIQYARRQGDNIAIVSPRVDVVVEISKRIKDAFLNEDI
                     DILHQQSSQQFEGHFVVCTVHQLYRFKQHFDTIFIDEVDAFPLSMDKSLQQALKSSSK
                     VEHATIYMTATPPKQLLSEIPHENIIKLPARFHKKSLPVPKYRYFKLNNNKIQKMLYR
                     ILQDQINNQRYTLVFFNNIETMIKTFSVYKQKITKLTYVHSEDVFRFEKVEQLRNGHF
                     DVIFTTTILERGFTMANLDVVVIDAHQYTQEALIQIAGRVGRKLECPAGKVLFFHEGV
                     SMNMIQAKKEIQRMNKLALKRGWIDE"
     gene            4355..5029
                     /locus_tag="PPY92_002134"
     CDS             4355..5029
                     /locus_tag="PPY92_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDJ2626117.1"
                     /translation="MNNCLSCGAKLYENITIYNLFKKPNRLCDRCKENWDNIKLDIKA
                     RRCSRCLKHLNQDEAYCLDCKFLSAHFNLMEQLYCQFQYDGLMKEMIHQYKFLKDYYL
                     CELLAHLIEIPQTSYDYIVPIPSSPAHDLSRTFNPVEAVLKAKGIRFDKILKMSNRPK
                     QSHLTKKERLADENPFIIDTELDLNGKEILLVDDIYTTGLTIHRAGCKLYAKNIRKFK
                     VFAFAR"
     gene            5090..5662
                     /gene="raiA"
                     /locus_tag="PPY92_002135"
     CDS             5090..5662
                     /gene="raiA"
                     /locus_tag="PPY92_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDJ2626118.1"
                     /translation="MIRFEIHGDNLTITDAIRNYIEEKIGKLERYFNDVPNAVAHVKV
                     KTYSNSATKIEVTIPLKNVTLRAEERNDDLYAGIDLINNKLERQVRKYKTRINRKSRD
                     RGDQEVFVAELQEMQETQVDNDAYDDNEIEIIRSKEFSLKPMDSEEAVLQMNLLGHDF
                     FVFTDRETDGTSIVYRRKDGKYGLIQTSEQ"
BASE COUNT         2055 a          834 c         1023 g         1936 t
ORIGIN      
        1 taaatataac gttaaaaaca attaatatcg atgaaggtga ataaatggtt acattattac
       61 tagttgcagt aacaatgatt gtcagtttga cgataacacc aattgttatt gcaatatcga
      121 aaagattaaa tttagttgat aaaccaaatt ttagaaaagt acacactaaa cctatttcag
      181 ttatgggtgg tacagtgatt ctcttttcat ttttaatagg tatttggatt ggtcatccta
      241 ttgaaacaga aatcaaacca cttattattg gtgcgattat tatgtacgta cttgggcttg
      301 tagatgatat ctacgatttg aaaccgtata taaaattggc tggtcaaatt gccgctgcct
      361 tagtagttgc tttttatggt gtgactattg attttatttc gttgccaatg ggtacaacga
      421 ttcattttgg atttcttagt attccaatta ctgtgatttg gattgttgct attacaaatg
      481 caattaactt aattgatgga ctcgatggtt tggcgtcggg tgtttctgca atcggactca
      541 ttacaatagg attcattgca attttacaag ctaatatttt cataacgatg atttgttgtg
      601 ttttattagg ctctttaatt gggtttttat tttacaattt ccatcctgcc aaaatatttt
      661 taggtgatag tggggcttta atgattggat ttatcatcgg attcctttct ttactcggat
      721 tcaaaaatat tacaattatt gcattgttct tcccaattgt tatcttagca gttccattca
      781 ttgatacttt gttcgcaatg attcgacgtg tgaaaaaagg gcagcatata atgcaagctg
      841 ataaatcgca tttgcatcat aaactattag ctttaggcta cacacataga caaacagtat
      901 tattaatcta ttcaatctct attttattta gtctttcgag cattattttg tatgtatcgc
      961 caccattagg tgttgtatta atgtttgtat taatcatatt tagtattgaa ttaattgttg
     1021 aatttacagg attaatagat aacaactacc gaccaatatt aaatttaatt agtcggaaat
     1081 cgtcacacaa agaagattag attttaaaac agtaagtaat aaaataaaaa aatgatgtct
     1141 gtacttttat gttgttataa aggaagacat actcacaaaa aacactagca tggaacgtgc
     1201 tttccaaatt gcaaagtaac aatacgcaat tagagttagc cctcctaagc tagtgttttt
     1261 atttttattc agttggtata tcgaaaggta actgctttgg agtttcttca gtcaaatcga
     1321 aatttccagc agtcatttga tttaaaaagt taataaacgc ttcatagtca cttttaacga
     1381 catcgatata gtagcttacc ttatcagtgt aagtttggtt tcttaacata aaatgagttg
     1441 aagctaattc atattcaaat ttaccagttt gatcataatt cagtgttact atacatggta
     1501 ctgcttctcg tagttcgaca cgcccgacat cataaatgac gtctctaaca gcaccgctat
     1561 aggcgcgaat taaaccgcca ccacctaatt taataccacc aaaatatctt gttactacga
     1621 cacacgcatt atgaacatcg agctttttta atatgtctaa cattgggaca ccggcagttc
     1681 ctgtcggttc accatcatca ttcgcttttt gaatattcat ttcaggtcca atagtatatg
     1741 cagaacaatt atgagtggca tctttatgtt ctttttttat tgcagcaata aatgctttag
     1801 cttcatcttc attttgaaca ggtttgatat gagcaatgaa tcttgattta ctaatcacat
     1861 tttcaataat gtgttctttt ttaacagtaa tgatattttg tgtcataata actccttaat
     1921 tcataagctt aagattattt aatcttcatt atacactgaa aatgacatga ctataaatcg
     1981 tttgattgcc attttctttt taactgaaat attgtatcat tgctatgagt atattttagg
     2041 aggacgacta tgaaaattgc tgtgatgacc gattctacaa gttatctgtc gcaggactta
     2101 atcgataaat ataatattca aatagcgcca ttaagtgtga cttttgatga tggaaagaac
     2161 tttacagaaa gtaatgaaat agcaattgaa gaattttata ataaaatggc atcgtctcaa
     2221 acgattccga caacaagcca accagcaatt ggcgaatgga ttactaaata tgaaatgcta
     2281 agagatcagg gttacacaga tatcattgtc atttgcttat ccagtgggat tagtggaagt
     2341 tatcaatcta gttatcaagc aggggaaatg gttgaaggtg ttaatgtaca tgcatttgat
     2401 agtaagcttg cagcaatgat tgaaggatgt tatgtattac gtgctattga aatggttgaa
     2461 gaaggatacg aaccacagca aattattgat gatttaacta atatgcgtga gcgcacaggt
     2521 gcatatttaa ttgttgatga cttaaagaat ttacaaaaaa gtggtcgaat tactggtgct
     2581 caagcatggg ttggaacatt attgaaaatg aagccagttc ttaagtttga agatggcaag
     2641 attataccag aagaaaaagt tcgtactaaa aagcgtgcca ttcaaacatt agaaaagaaa
     2701 gtattagata ttgtaaaaga ctttgaagaa gtaactttat ttgtcataaa tggagatcat
     2761 tttgaagatg gtcaagcgtt atacaaaaag ttacaagaag attgtccttc aggttatcaa
     2821 gtagcatact ctgagtttgg tccagttgtt gcagcacatt taggttctgg tggattaggt
     2881 ttaggctatg ttggcagaaa aataagatta acataattat aaaattttaa taaaagagtc
     2941 tatattgtaa ttggaaatta tctctcgtat acatggcttt aaatgttcat catttgaaag
     3001 tcaaaatgct aaagatataa gaaaatcatt ataatattag gctctttttt acgttgaaat
     3061 gaggttttaa gcattaaaca ttacgggaaa ttaattcatc ctcatacttc acttactaat
     3121 gaaaaaataa aaaaagaagt aacaggtgtc atcaaacaaa attcaaacta ttattgtgtt
     3181 caatgtgaaa gtacaaatcc aaagcatttt tatcagtatg attcctcagt acattccaag
     3241 aaaattgtat attgcagaaa ttgtatatca ctgggtcgaa tggataatgt aacaagatat
     3301 aaaataacag agagttcgca aagttcatca caagcatatt atcatctctc atttgaattg
     3361 tcggaacagc agtcttatgc ctcagaacat attgttcgag ccattagaat gagacaaacg
     3421 attttgttat atgccgtaac aggtgcaggt aagacagaaa tgatgtttca aggcattcaa
     3481 tatgcaagac gacagggaga taatatagct attgtgtcac cgcgtgtaga tgttgttgta
     3541 gaaattagta aacgtattaa agacgcattt cttaatgaag atatagacat actacaccag
     3601 caatcaagtc aacaatttga agggcatttt gttgtatgca cagtacatca actttaccga
     3661 ttcaaacagc actttgatac tatttttatt gatgaagtcg atgcctttcc tttatcaatg
     3721 gataaaagtt tacaacaagc attgaagtca tcttctaaag ttgaacatgc aacgatttat
     3781 atgacagcaa caccaccgaa acaacttctg tcagagattc cccacgaaaa tataattaaa
     3841 ttgccagctc gctttcataa aaaatcactt ccagttccta aatatcgtta tttcaaactt
     3901 aataataata agattcagaa aatgttatac cgaattttac aagatcaaat taataatcaa
     3961 cgttatacac tggtgttttt taacaatata gaaacaatga ttaaaacatt ttcggtttat
     4021 aagcagaaaa ttactaaatt aacatacgtc catagcgagg atgtttttcg ctttgaaaaa
     4081 gttgaacaat taaggaatgg acatttcgat gtcattttta ctacgacaat attagaacgt
     4141 ggatttacaa tggcaaattt ggatgttgtt gttatcgatg cacatcaata tactcaagag
     4201 gctttaatac aaattgctgg acgtgttgga cgaaaattag aatgtcctgc tggaaaagta
     4261 ttgttttttc atgaaggagt aagtatgaat atgattcaag ctaaaaaaga gattcaaagg
     4321 atgaacaaat tagcattaaa aagaggttgg attgatgaat aattgtttga gttgtggtgc
     4381 taagttatat gaaaatataa ccatttataa tttgttcaag aaacctaata gattatgtga
     4441 cagatgcaaa gagaattggg acaatattaa acttgatatt aaagcaaggc gatgttcaag
     4501 gtgcttaaaa cacttaaatc aagatgaagc gtattgttta gactgcaagt ttctatcggc
     4561 acactttaat ttaatggaac aattatattg tcaatttcaa tatgacggtt taatgaaaga
     4621 gatgatacat cagtataaat ttttgaaaga ctattattta tgtgaattat tggcacattt
     4681 gattgaaata ccacaaacat cttatgacta tattgtgcca attccttctt cgccggcaca
     4741 tgatttatct agaacattta acccggtaga agcagtacta aaagctaaag ggattcgctt
     4801 tgataagatt ttaaagatgt caaatagacc aaaacagtct catttaacta agaaagagcg
     4861 tctggcagat gaaaatccat ttattattga tacggaatta gatttaaatg gcaaggaaat
     4921 attactcgtt gacgatattt atacaactgg attaacaatt catcgtgcag ggtgtaaatt
     4981 atatgctaaa aatatcagaa aattcaaagt gtttgcgttt gcacgatagc gtaaaaatgt
     5041 taaaatataa taaagagtta ccaataaaga ggtttaagga gagattacta tgattagatt
     5101 tgaaattcat ggagataacc tcactatcac agatgctatt cgcaactata ttgaggaaaa
     5161 aattggtaag ttggaacgtt attttaatga cgtaccaaat gcagtggcgc atgttaaagt
     5221 taaaacttat tcaaattcag ctactaaaat tgaagtaaca attccattga aaaatgttac
     5281 gttaagagct gaagagcgaa acgatgattt atacgcaggt attgatttaa ttaataataa
     5341 acttgaaaga caagttcgaa aatataaaac acgtattaat cgtaagagcc gtgatcgagg
     5401 agatcaagaa gtgtttgttg ccgaattaca agaaatgcaa gaaacacaag ttgataatga
     5461 cgcttacgat gataacgaga tagaaattat tcgttcaaaa gaattcagct taaaaccaat
     5521 ggattcagaa gaagcggtat tacaaatgaa tctattaggt catgacttct ttgtattcac
     5581 agacagagaa actgatggaa caagtatcgt ttaccgacgt aaagacggta aatatggctt
     5641 gattcaaact agtgaacaat aaattaagtt taaagcactt gtgtttttgc acaagtgctt
     5701 ttttatactc caaaagcaaa ttatgactat ttcatagttc gataatgtaa tttgttgaat
     5761 gaaacatagt gactatgcta atgttaatgg atgtatatat ttgaatgtta agttaataat
     5821 agtatgtcag tctattgtat agtccgag
//
LOCUS       DANBWH010000164         5826 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.170, whole genome shotgun sequence.
ACCESSION   DANBWH010000164 DANBWH010000000
VERSION     DANBWH010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5826
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.170"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(113..310)
                     /locus_tag="PPY92_002136"
     CDS             complement(113..310)
                     /locus_tag="PPY92_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626119.1"
                     /translation="MNCYDEIFNTIKKLIENKEISSYQINKDTGISYGNINAMRRGER
                     RIENLSLKNAKILYEYAKKVL"
     gene            complement(803..2143)
                     /gene="glnA"
                     /locus_tag="PPY92_002137"
     CDS             complement(803..2143)
                     /gene="glnA"
                     /locus_tag="PPY92_002137"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDJ2626120.1"
                     /translation="MPKRTFTKEDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQL
                     EKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTD
                     GTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFD
                     LAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKL
                     VVKTIARKHNLHATFMPKPLFGVNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQF
                     TAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRI
                     EVRSVDPAANPYMALAAILEAGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPS
                     TLYTALKAMRENEVIKKALGNHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY"
     gene            complement(2162..2530)
                     /locus_tag="PPY92_002138"
     CDS             complement(2162..2530)
                     /locus_tag="PPY92_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDJ2626121.1"
                     /translation="MISNDAIRRNMAVFSMSVVSKLTDLTPRQIRYYETHELIKPERT
                     EGQKRLFSLNDLERLLEIKSLLEKGFNIKGIKQIIYDSQEHLTTDEQEIRKKMIVDAT
                     QKPIGETLPINRGDLSRFIK"
     gene            complement(2773..4011)
                     /locus_tag="PPY92_002139"
     CDS             complement(2773..4011)
                     /locus_tag="PPY92_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDJ2626122.1"
                     /translation="MKDISKIVADVESTLAPYFKEIEETAYINQEKVLNAFHHVKATE
                     SDLQGSTGYGYDDFGRDHLEEIYAQAFKAEDAIVRPQIISGTHAITIALQSLLKHGDE
                     LIYITGSPYDTLLEVIGVNGNGIESLMEHGVSYKDIALKEGKIDIESVLDGVSERTKV
                     IAIQRSKGYDQRPSIPLDEIEKVITRLKNVHPNILIFVDNCYGEFVERREPIECGADL
                     IAGSLIKNPGGGLAKIGGYIAGRKDLIERCGYRLTAPGIGKEAGASLNALLEMYQGFF
                     LAPHVVSQSLKGALFTSLFLEKMNMNTTPKYYEKRTDLIQTVKFETKEQMISFCQSIQ
                     HASPINAHFSPEPSYMPGYEDDVIMAAGTFIQGSSIELSADGPIRPPYEAYVQGGLTY
                     EHVKIAVTRAVNQLKEQGLI"
     gene            complement(4030..5268)
                     /gene="hflX"
                     /locus_tag="PPY92_002140"
     CDS             complement(4030..5268)
                     /gene="hflX"
                     /locus_tag="PPY92_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDJ2626123.1"
                     /translation="MAQQQIHDTKNKLEKAVLVGVHAQDDKQFNFESTMEELSSLSET
                     CQLEVLGQITQNRDRVDRKYYVGKGKIEEIQAFIEFKDIDVVITNDELTTAQSKSLNE
                     ALGVKIIDRTQLILEIFALRARSKEGKLQVELAQLDYLLPRLQGHGKSLSRLGGGIGT
                     RGPGETKLEMDRRHIRTRMNEIKHQLRTVEEHRERYRNKRNQNQVFQVALVGYTNAGK
                     SSWFNVLANEETYEKDQLFATLDPKTRQIQINDGFNLIISDTVGFIQKLPTTLIAAFK
                     STLEEAKGADLLVHVVDSSHPEYRTQYDTVNDLIKQLDMSHISQIVIFNKKDLCDHAS
                     NRPASDLPNVFVSSKNDGDKLLVKTLFIDEIKRQLTYYDETIATNNADRLYFLKQHTL
                     VTELKYDEIENVYRIKGFKK"
     gene            5380..>5826
                     /locus_tag="PPY92_002141"
     CDS             5380..>5826
                     /locus_tag="PPY92_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDJ2626124.1"
                     /translation="METIYDFVVETNKGVTYKLDAYKGDVMLIVNTASECGFTSQFEG
                     LQSLYEKYKDQGFVILGFPCNQFGGQEPGSGEEAAQNCKLNYGVTFPMHQKIDVKGEH
                     QLPLFRYLTAAQHGFFNEKIKWNFTKFLVDREGNVVKRFAPQKKPVQ"
BASE COUNT         1808 a         1088 c          823 g         2107 t
ORIGIN      
        1 aatcactaaa catgatttct ttcactttta gaaaactttt aatactataa aaacacccac
       61 tcagtcacta gtttgggcag ttattgtatg cctattgaac tcaatgctta tattacaata
      121 cctttttcgc atattcatat aagatctttg cattctttaa gcttaaattt tctattcttc
      181 tttccccacg gcgcatagca ttaatattac cgtaacttat cccagtatct ttattaattt
      241 gataacttga tatctctttg ttttctatca attttttgat tgtattgaat atttcatcat
      301 agcaattcat aaattagatg aggcgaaatt tttaattttt tagaatatca atagtagtat
      361 aactaaaatg aaaataccga tcgataaaca aaaagatatt ttttgttttg tttctctttt
      421 catatagtat taccccctta ataatgcgta gtaaggtccc tcttttcggg gtcttacctt
      481 agaacgttct gcaaatgaat tcgatgagaa gtaatatgaa tatggctatt ttcaagtaat
      541 actcaacgtt ttcgcgacgt tcttttatcg cctcatctca tcacctccaa atatattaaa
      601 attcatgtga actaaaatat aaaatggtct tccccagctt taaaaaaata aatacataaa
      661 acatttactt gaccaaaact tgaccaaatc cattttttaa caaaagtggt caagtgaaat
      721 cccatctttc caaatcaaaa aagagctaaa gcaaaacgct ctagcccttg atattactga
      781 tttcccatca ttgtttagcg tattaatatt gcttcatgta ctgatctctt tcccattcag
      841 atacttgagt tctgtagtaa tcccattcaa ttgattttga attaataaat tgattataga
      901 tatgatttcc taaagctttt ttaataactt cattttcacg cattgctttt aatgcagtat
      961 aaagtgttga aggtaagtct tgaatgccta ctgcttcacg ttcttcacgg ttcatttcgt
     1021 aaatattttg gttaactggt tctggaactt ttaatttatt tttaatacca tctagtccag
     1081 cttctaagat tgcagctaac gccatgtatg ggtttgcagc tggatctact gaacgtactt
     1141 cgatacgagt agataatcct cttgaagatg gtacacggat taatggtgaa cggtttttac
     1201 cactccatgc aatataacat ggtgcttcat aaccaggtac taaacgttta tatgagttta
     1261 ctaacgggtt acatacagca gtaaatccgc gtgcattttt aagcacacct gctgtaaatt
     1321 gatatgcagt ttccgttaag cccatttcag tatttggatc aaagaatgca ttttctttac
     1381 ctttgaataa tgaaacgtta aagtgcatac cgctaccatt cacaccgaat aatggtttag
     1441 gcataaatgt tgcgtgtaaa ttatgtttac gtgcgattgt tttaacaacc aatttaaatg
     1501 tttggatatt atcacatgct gtaacagcat ctgcatattt aaagtcaatt tcatgttgac
     1561 caggggcaac ttcatggtga ctagcttcaa tatcgaagcc catatcctct aattctaaaa
     1621 caatatcacg acgacagttt tcacctaaat ctgtaggtgc taaatcgaaa tatccaccat
     1681 catcattaag ttctaaagtt ggttcccctt tttcatccaa cttaaacaag aagaattctg
     1741 gttcaggccc taggttaaag tctgtgaagc ctaaatcttc catttctttt aatacacgtt
     1801 ttaagtttgc acgaggatcc ccttcaaatg gtgttccatc tgttttatat acatcacaaa
     1861 ttaaacgtgc aactttacct tgtccagcag tccatgggaa gattacccaa gtatctaaat
     1921 ctggatgtaa gtacatatct gattcttcga tacgtacgaa accttcgata gaagaaccgt
     1981 caaacatcat ttcgttatca agtacttttt ctaattggct tacaggcact tcaacatttt
     2041 taattgttcc taaaatatca gtgaattgta atcttaaata tcttacattt tcctcttctg
     2101 caaatttacg aatgtcttct ttagtgaaag tacgttttgg cattttaaaa tcctccaaat
     2161 tttatttaat aaatcgggat aaatcaccac gatttattgg caaagtttct ccaataggct
     2221 tttgcgtggc atctacaatc atcttttttc ttatctcttg ttcatctgtt gttaaatgct
     2281 cttgtgagtc ataaatgatt tgtttaatcc ctttgatatt aaatcctttt tctaataatg
     2341 atttaatttc tagtaatctt tccaaatcat tgagtgagaa cagacgtttt tgaccttctg
     2401 ttctttcagg tttgatgagt tcatgtgttt catagtaacg tatttgcctt ggcgttaaat
     2461 ccgttaactt acttactaca ctcatagaga agacagccat atttcgtctg attgcatcat
     2521 tcgatatcat tgttcctctc ctctactttt gaacttgtta ataacttatc ataatttttt
     2581 taggatttca aaaatatgtc aggttttcta acataaagct taaaacattg atataactgc
     2641 gatttcgact tctaaaacga cataaaacaa tctacagcta aaatattttt tcaattttta
     2701 aaaataatct gataaatctc cgcaaaaaat ttcacccaag acacattatc atttagggtg
     2761 aaatttaatc tattatataa gtccttgttc tttcaactga ttaacagctc ttgtcacagc
     2821 aattttaacg tgttcatatg ttaatcctcc ttgaacatat gcttcataag gaggacgaat
     2881 aggtccatct gcagataatt caatcgatga accttgaata aacgtaccag ctgccataat
     2941 aacatcatct tcgtaaccag gcatataact aggttctgga ctaaaatgtg cattaattgg
     3001 ggatgcgtgt tgaatacttt gacaaaatga aatcatttgt tctttcgttt caaatttaac
     3061 tgtttgaatt aaatcagttc gtttttcgta gtacttcggc gttgtgttca tattcatttt
     3121 ttctaaaaat aaactagtaa acaatgcacc tttaagactc tgactgacaa cgtgtggtgc
     3181 taaaaagaaa ccttgataca tttcaagcaa tgcatttaat gatgcacccg cttctttacc
     3241 aataccaggt gctgtcaatc tataaccaca tcgttcaatt aaatcttttc taccagcaat
     3301 gtatccacca atcttagcta aaccaccgcc agggttttta attaatgatc ctgctattaa
     3361 atcggcacca cattctatag gttcacgtct ttcaacaaat tccccataac agttatccac
     3421 aaatattaaa atattaggat gcacgttttt caacctagta attacctttt caatttcatc
     3481 tagcggaatt gaaggtcttt gatcatagcc tttcgaacgt tgaatcgcta ttactttggt
     3541 gcgctcagaa accccatcta acacactttc aatatcgatc ttaccttctt taagtgcaat
     3601 atctttatac gatacgccgt gctccattaa actttcaata ccatttccgt ttacgccaat
     3661 gacttcaagt aaagtgtcat atggactacc cgttatataa attaattcat caccatgttt
     3721 taataaactt tgtaatgcaa tagtaatcgc atgcgtacct gaaataattt gcggacgaac
     3781 aattgcatct tctgctttaa atgcctgcgc atatatttct tctaaatgat cacgtccaaa
     3841 gtcatcatac ccgtatcctg ttgatccttg tagatcactt tcggttgctt tgacatgatg
     3901 aaatgcattt aatacttttt cttgattaat atatgctgtt tcttcaattt ctttaaaata
     3961 tggtgctaac gttgattcga cgtcagctac tatcttactt atatctttca tttgaatttc
     4021 gtccttttat tattttttaa atccttttat acgataaaca ttttcaattt catcatattt
     4081 aagttcagtc actaatgtat gttgttttag aaaatataat cgatctgcat tattcgtcgc
     4141 aattgtctca tcataataag ttaattgcct tttgatttca tcaataaata acgtcttaac
     4201 aagtaattta tcaccatcat ttttagaaga aacaaaaaca ttaggcaaat cacttgctgg
     4261 acgatttgat gcatgatcac ataagtcctt tttattaaaa ataactattt gagaaatatg
     4321 actcatatct aattgtttga ttaaatcatt aactgtgtca tactgcgtac ggtattcagg
     4381 atggctacta tctacgacat gtactaataa atctgcacct ttagcctctt ctaaagttga
     4441 tttaaaagct gcaattaacg tcgtaggtag tttctgtata aaaccaacag tatctgaaat
     4501 aattaaatta aatccatcat ttatttgaat ttgtcgtgtt ttaggatcta acgttgcaaa
     4561 taattgatct ttttcatacg tctcttcatt tgctaaaaca ttaaaccatg atgatttacc
     4621 agcatttgta taaccaacta aagctacttg aaacacctga ttttgatttc ttttatttcg
     4681 atatctttcg cgatgttctt ctaccgtccg caattgatgt ttaatttcat tcatacgagt
     4741 tcgaatatgt ctgcgatcca tctctaactt cgtttcacca gggcctctag ttccaatacc
     4801 gccacctaaa cgagaaagac ttttaccatg gccttgcaat ctaggtaata aataatcaag
     4861 ttgtgctagc tctacttgca atttaccttc tttacttctt gctcttaatg caaatatttc
     4921 aagaatcaac tgagttctat caataatttt tacacctaaa gcttcattta gtgatttgga
     4981 ttgtgcagtc gttaattcat catttgtgat gactacatca atatctttga actcaataaa
     5041 tgcttgaatt tcttcaattt tacctttacc aacataatat ttgcgatcta cacgatctct
     5101 gttttgagta atttgaccca acacttcaag ttggcaagtc tctgataaag atgataattc
     5161 ctccattgta gactcaaaat taaattgctt atcatcttga gcatgtacac cgactaagac
     5221 agctttttct agtttatttt tagtatcatg aatttgttgc tgagccatgt tatacatctc
     5281 cttaataaaa tcctactcaa agtattctat catagagttt tagtaaagac tattacacga
     5341 ttttatggta cattttaagt aaatcgaaag gatgattcaa tggagacaat ttatgatttt
     5401 gtagtagaaa caaataaagg tgttacttac aaattagatg catataaggg tgacgttatg
     5461 ctaattgtta atacagctag cgaatgtggc tttacttcac aatttgaagg tttgcaatca
     5521 ttatacgaga aatacaaaga tcaagggttt gtaattctag gtttcccttg taatcaattt
     5581 ggtggtcaag aacctggatc aggcgaagaa gcagctcaaa actgtaaatt aaactatggt
     5641 gttaccttcc ctatgcatca aaaaatagac gtaaaagggg aacatcaatt acctttattt
     5701 agatatttga ctgcggcaca acacggattc tttaatgaga aaatcaaatg gaatttcact
     5761 aagtttttag tggatcgcga aggtaacgtt gttaaacgat ttgcacctca aaaaaaacct
     5821 gttcaa
//
LOCUS       DANBWH010000165         5815 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.171, whole genome shotgun sequence.
ACCESSION   DANBWH010000165 DANBWH010000000
VERSION     DANBWH010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.171"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..189)
                     /locus_tag="PPY92_002142"
     CDS             complement(<1..189)
                     /locus_tag="PPY92_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626125.1"
                     /translation="MHHVNKYFNQTMVIEALKMSFYKLNPKQLIKNPIMFVVEVGMVL
                     TLILICFPDIFGTSYLSRG"
     gene            complement(208..1884)
                     /gene="kdpA"
                     /locus_tag="PPY92_002143"
     CDS             complement(208..1884)
                     /gene="kdpA"
                     /locus_tag="PPY92_002143"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit A"
                     /protein_id="HDJ2626126.1"
                     /translation="MEIILFLTMMVMITYVFSGYLYRVALVQSSRVDLIFTRFENMCF
                     KIIGTDLEHMSAKTYVKHFLAFNGFMGFITFVLLIVQQWLFLNPNHILNQSIDLAFNT
                     AISFLTNSNLQHYNGESDVTYLTQMIVMTYLMFTSSASGYAVCIAMLRRLTGLTNIIG
                     NFYQDIVRFIVRVLLPLSCLISILLMTQGVPQTLHANLMIRTLSGHIQHIAFGPIASL
                     ESIKHLGTNGGGFLAGNSATPFENPNIWSNFIEMGSMMLLPMSMLFLFGRMLSRHGKR
                     VHRHALILFVAMFFIFIAILTLTMWSEYRGNPILANLGIYGPNMEGKEVRFGAGLSAL
                     FTVITTAFTTGSVNNMHDSLTPIGGLGPMVLMMLNVVFGGEGVGLMNLLIFVLLTVFI
                     CSLMVGKTPEYLNMPIGAREMKCIVLVFLIHPILILVFSALAFMIPGASESITNPSFH
                     GISQVMYEMTSAAANNGSGFEGLKDDTTFWNISTGIIMLLSRYIPIILQLMIASSLVN
                     KKSYHQDKYTIAIDKPYFGVSLIVFIVLLSGLTFIPVLLLGPIGEFLTLK"
     gene            complement(1908..2018)
                     /gene="kdpF"
                     /locus_tag="PPY92_002144"
     CDS             complement(1908..2018)
                     /gene="kdpF"
                     /locus_tag="PPY92_002144"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDJ2626127.1"
                     /translation="MLLCCKEVMAMLIVLLLVVIALVLYLFYALIRSEKF"
     gene            2156..4813
                     /locus_tag="PPY92_002145"
     CDS             2156..4813
                     /locus_tag="PPY92_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HDJ2626128.1"
                     /translation="MSNTESLNIGKKRGSLTIYIGYSPGVGKTFEMLSNAIELFQSNV
                     DIKIGYIEPHQRDETNALAEQLPKITTNFTKHGSHHFQYLDVDRIIEESPTIVLIDEL
                     AHTNISRDRHEKRYMDIEEILNHGIDVHTTLNIQHIESLSSQIELMTGVHVKERVPDY
                     FIMSADVLEVVDISPEQLIKRLKAGKVYKKDRLDVAFSNFFTYAHLSELRTLTLRTVA
                     DLMSDKEKVRHNHKTSLKPHIAVAISGSIYNEAVIKEAFHIAQKEHAKFTAIYIDVFE
                     KNRQYKDSQKQVHQHLMLAKSLGAKVKVVYSQTVALGLDEWCKNQDVTKLIIGQHIRN
                     KWRDFFNTPLIDHLMSFEHSYKIEIVPIKQIPVELKMNKSPYRPKGKRFAIDMLKMIL
                     IQIICVMMGLWIYQLDKHESSTIILMIFLIGIILLSIWTRSFIIGFLAAIINVFVFNY
                     FFTEPRYTFEVYRFDYPITFIVSILTSILTSALLKQIKFQYSITKKQLYRTDLLFQFN
                     DSIKQTYTVENLLINAGYQINQLLQQSITIYVINQSKVIKTIPLQNHIDNTTQQHEQA
                     LSWVIKNERQAGATTDTFPGINKWLIPIGTSPIKGILAIDYQSSQVINPYDASILESM
                     LNELSLAVENVTLLKQTRESMLQAERQLTHSNFLRSISHDIRTPLTTIMGNLDILVSH
                     SKDMSIIEKEQLLVHSFQESQYLYLLVTNILSLTKLQSSNVQIKLQPYLVSELVEEID
                     MILERRHLKKRITVSSSVNLQFIHIDSKLILQALFNLIENAVKHTSTDTKINLSIRYA
                     SYEQIEFAVIDEGPGISLEEQQKIFEPFYTGSNKYFKDNQKESMGLGLYLVQTILHKH
                     QSNLQYKPNQPHGSIFYFNIYTDFNEGDV"
     gene            4813..5508
                     /locus_tag="PPY92_002146"
     CDS             4813..5508
                     /locus_tag="PPY92_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDJ2626129.1"
                     /translation="MQSKILIIEDDHAITHLLDVALTLDYYNVTTADNATQAHFKIQI
                     DKPDVILLDLGLPDKDGLCLISEIRQHTDIPIIVISARQEEQTIIQALDNGANDYMTK
                     PFNVDELRARIRVIERIAKSHQETNIVFTNGLLSIDFGSKSVVINNQEVHLTPNEFSL
                     LELLSNHKGKVLTYEMILKRIYGYVNKTEMPSLRVHMTSLRQKLSQCHEDAKDIIKTH
                     PRIGYQMLQWKEK"
BASE COUNT         2169 a          984 c          851 g         1811 t
ORIGIN      
        1 acctcgcgat agatagctag ttccaaatat gtcaggaaaa catattaata tcaacgttaa
       61 aaccattcca acctcaacga caaacattat cggatttttt attaattgtt taggattcag
      121 cttataaaaa ctcattttca aagcttctat caccattgtt tgattgaaat atttatttac
      181 atgatgcatt ttttccatcc ctttacttta ttttaaagtt aaaaattcac caataggacc
      241 aagtaatagt actggaataa atgtcaaacc acttagtaaa acgataaata cgattagtga
      301 tacgccaaaa taaggtttat caatcgctat tgtatattta tcttgatggt atgatttttt
      361 attcactaaa cttgatgcaa tcattaattg caaaataatt ggtatataac gagaaagcaa
      421 cataatgatt cctgtagaga tattccagaa tgttgtatca tctttcagtc cttcaaaccc
      481 tgatccattg ttcgcagcag ctgatgtcat ttcatacata acttgtgaaa taccatgaaa
      541 agacggattc gttatacttt cacttgctcc aggaatcata aaagcaagtg ctgaaaatac
      601 taaaattaaa attgggtgta tgagaaagac taagacaata catttcattt cacgggcgcc
      661 aattggcata tttaaatatt ctggtgtttt accaaccatc aaactgcata taaacaccgt
      721 cagtaagaca aatatcaata aattcatgag tcctacgcct tcgccaccaa atacaacatt
      781 tagcatcatt aataccattg gtcctaatcc acctataggc gttaagctat catgcatgtt
      841 attaacagaa cccgttgtaa atgccgtcgt aataactgta aatagtgctg acaaacctgc
      901 tccaaaccgt acctctttac cttccatatt cggtccataa atgcctaaat tcgctagtat
      961 tggattacca cgatactcac tccacatagt taatgtaaga attgctataa aaatgaaaaa
     1021 cattgcgaca aataatatca acgcatgacg atgtactcgt ttaccatgtc tacttaacat
     1081 gcgaccaaat aagaacaaca ttgacatagg aagtaacatc atactgccca tttctataaa
     1141 attgctccaa atatttggat tttcaaaagg tgttgcagaa tttcctgcta aaaatcctcc
     1201 accattcgta ccaagatgtt ttattgattc aagtgatgca ataggtccaa atgcaatatg
     1261 ttgaatatgt ccgcttaaag tccgaatcat taaattagca tgcaacgttt gtggtacacc
     1321 ttgagtcatc aataaaatac taattaaaca tgataatggt aaaagtactc ggacaataaa
     1381 ccgaacaata tcttgataaa aattaccaat gatattagtt aatccagtta aacgtctcaa
     1441 catcgctata caaacggcgt aacctgatgc actagatgta aacattaaat atgtcattac
     1501 aatcatttgc gttaaatatg tcacatctga ttcaccgtta tagtgttgta aattactatt
     1561 tgttaaaaaa gatattgctg tattaaacgc taaatctatc gattggttta aaatatgatt
     1621 tggatttaaa aaaagccatt gctgaactat tagcaataca aatgttataa accccataaa
     1681 tccattaaat gccagaaaat gtttgacata tgttttagct gacatgtgtt ctaaatctgt
     1741 gccgataatt ttaaaacaca tattttcaaa tctagtaaat attaaatcta ctcttgacga
     1801 ttgcaccaat gctacgcgat atagatatcc actaaaaaca tacgtaatca taaccatcat
     1861 tgttagaaac aaaattattt ccatgataac cctcacttaa tatatttcta aaatttttca
     1921 ctacgaatta aggcataaaa taaatacaaa actaatgcaa taactaccag taataaaacg
     1981 atgagcattg ccataacctc cttacaacac aacaacatcg taacaacttg tttatgagag
     2041 aaatattaat tttcaaactt agttattaag aaatcattaa gatgtgtatg cagaaataaa
     2101 ttttatagca tttaattgtg aagaatatta tgatattgct atcgaggtga aggttatgtc
     2161 aaacactgaa tcgctaaaca taggaaaaaa gcgtggatct ttaacaattt acatcggtta
     2221 cagtccaggt gttggtaaaa catttgagat gctttcaaat gccattgaac tatttcaaag
     2281 taacgttgat attaaaatag gatatattga accgcatcag cgtgatgaaa caaatgcatt
     2341 agctgaacaa ttacctaaaa tcacaaccaa ttttactaaa catggtagtc atcattttca
     2401 atatttagat gtcgaccgca taatcgaaga atcgccgaca atagtactta tcgatgagtt
     2461 agcacatacg aatatttcta gagatcgtca tgagaaacga tatatggata ttgaagaaat
     2521 tttaaatcat ggtatcgatg ttcataccac tttgaacatt caacatattg aaagtttaag
     2581 tagtcaaatt gaactgatga ccggtgtaca tgttaaagaa cgtgtacccg actatttcat
     2641 aatgagcgcc gatgtattag aagtcgtaga tatctcacct gaacaattaa ttaaacgctt
     2701 aaaagctggc aaggtatata aaaaggatag gctagatgta gcatttagta atttctttac
     2761 gtatgcccac ctaagcgaac tgcgtacatt gacgttaaga acagttgccg acttgatgag
     2821 tgataaagaa aaagtccgac acaaccataa aacgtcactc aaacctcata ttgctgtggc
     2881 aattagtggg agcatttata atgaagcagt aattaaagag gcattccata ttgctcaaaa
     2941 agaacatgcg aagttcactg ctatttatat agatgtattc gaaaaaaaca ggcaatataa
     3001 agatagtcaa aagcaagtgc atcaacatct catgcttgca aaatcattag gagcaaaagt
     3061 aaaagtagtt tatagccaaa ccgttgcatt aggattagac gaatggtgta aaaatcaaga
     3121 tgtaaccaaa ttaattatcg gacaacatat tagaaataag tggcgagact ttttcaatac
     3181 acctttaatt gaccatttaa tgtcctttga acatagctat aaaatcgaaa tcgttccaat
     3241 caaacaaata cctgttgaat tgaaaatgaa caaatcaccc tatcgtccta aaggcaaacg
     3301 tttcgccata gatatgttaa aaatgatttt gattcaaata atttgtgtaa tgatgggact
     3361 gtggatttat caacttgata agcatgagtc tagtacgatt attttaatga tttttctcat
     3421 cggcatcatt ttattatcca tttggacgcg gtccttcatc attggctttt tagcagcaat
     3481 tattaacgta tttgttttta attatttttt tacggaacct agatatacat ttgaagtata
     3541 tcgctttgac tatcctatta catttatcgt tagcatttta acaagtattt taacgagtgc
     3601 tttattaaaa caaattaaat tccaatactc cattactaaa aagcaacttt atcgtacaga
     3661 tttattattt caatttaatg attcgattaa acaaacttat acagttgaaa acttactaat
     3721 aaatgctgga taccaaatta atcaattatt gcaacaatca attactatat acgttatcaa
     3781 tcaatcgaaa gtaattaaaa caataccatt gcaaaaccat atcgataata cgactcaaca
     3841 gcatgaacaa gcattaagct gggtaattaa aaatgaaaga caagctggtg caacaacaga
     3901 tacatttcca ggcattaata aatggttgat accaattggc acatctccga ttaaaggcat
     3961 tctagccatt gattaccaaa gttctcaagt gattaatcca tatgatgcat caatattaga
     4021 atcaatgtta aatgaacttt ctctcgccgt tgaaaatgtg acgttactta aacaaacgag
     4081 agaatccatg ttacaggctg aacgtcaact gacacattca aattttttaa gatcaatttc
     4141 gcatgacata cgtacaccgt taacaacaat tatgggaaat ttagatattt tagtatcaca
     4201 tagtaaagat atgtctatca tagaaaagga acaattactt gtacatagtt ttcaagaaag
     4261 tcagtattta tatctattag tgacaaatat tttatcatta acaaaattac agtcatcaaa
     4321 tgttcagata aaattgcaac cttatctcgt tagtgaatta gtagaagaaa tcgatatgat
     4381 tttagaacgt cgccatttaa aaaagcgtat aactgtatca tcctccgtaa atttacagtt
     4441 tatacacatt gacagtaagt tgattttaca agctttattc aatttgattg aaaatgcagt
     4501 taaacacact tctactgaca ccaaaatcaa tttatctatt cgttatgcta gctatgaaca
     4561 aatcgaattt gccgttatag acgaaggacc tggaattagt ttagaagaac aacaaaagat
     4621 attcgagcct ttttacacag gatcaaataa atattttaaa gataaccaaa aagaaagcat
     4681 gggactaggc ttatatttag tacagactat cctacacaaa catcaatcaa acttacaata
     4741 taaaccgaac caaccacacg gcagtatatt ctatttcaat atatatacag actttaacga
     4801 aggagacgta taatgcaatc taaaatattg ataattgaag atgatcacgc aatcacacat
     4861 ttacttgatg ttgcattaac tttagattat tacaatgtaa ctacagccga caatgccaca
     4921 caagcacact ttaaaattca aattgataaa ccagatgtca ttttattaga tttaggttta
     4981 ccagataaag atggattatg tttgatttca gaaatcaggc aacatactga cattcctatc
     5041 attgtaataa gtgcaagaca agaagaacaa acaattattc aagctttaga taacggtgcg
     5101 aatgactata tgactaaacc ttttaatgtt gatgagcttc gggcacgaat acgagtcatc
     5161 gagagaatcg ctaagtcaca tcaagaaact aacattgtat ttactaacgg tttgctaagc
     5221 atcgactttg gctccaaatc tgttgttatt aataatcagg aggtacattt gacgccaaat
     5281 gaatttagct tgttagaact attgtcaaat cataaaggaa aagtactcac ttatgagatg
     5341 atattaaaac gaatatatgg ctatgttaat aagactgaaa tgcctagttt aagagtgcat
     5401 atgacatcat taagacaaaa actttcacaa tgtcatgagg acgcaaaaga tattattaaa
     5461 acacatccac gcattggtta tcaaatgttg cagtggaaag agaaataatt aaataaaaaa
     5521 gatcgctgcc attaaacgaa tgattggcag tgatcttttt tatattcaac ttattattta
     5581 tcgcgcacta gcgtcagcaa aaagccctat tgttttttat aagaacatga tactgaaatt
     5641 tcataaagtt tatttatgtt cgattattct taatttaagt agttgttata ttctagattc
     5701 atatttatat ttacacagaa tgatataacc gcatacagat ctcaatccaa ttaaatattt
     5761 aacctttaaa acgttagtac tttgatatct acagagaatc aattattgaa aacaa
//
LOCUS       DANBWH010000166         5790 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.172, whole genome shotgun sequence.
ACCESSION   DANBWH010000166 DANBWH010000000
VERSION     DANBWH010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.172"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            152..1156
                     /gene="aaa"
                     /locus_tag="PPY92_002147"
     CDS             152..1156
                     /gene="aaa"
                     /locus_tag="PPY92_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin/adhesin Aaa"
                     /protein_id="HDJ2626130.1"
                     /translation="MQKKVIAAIIGTSAISAVAATQANAATTHTVKPGESVWAISNKY
                     GISIAKLKSLNNLTSNLIFPNQVLKVSGSSNSTSNSSRPSTNSGGGSYYTVQAGDSLS
                     LIASKYGTTYQNIMRLNGLNNFFIYPGQKLKVSGTASSSNAASNSSRPSTNSGGGSYY
                     TVQAGDSLSLIASKYGTTYQKIMSLNGLNNFFIYPGQKLKVTGNASTNSGSATTTNRG
                     YNTPVFSHQNLYTWGQCTYHVFNRRAEIGKGISTYWWNANNWDNAAAADGYTIDNRPT
                     VGSIAQTDVGYYGHVMFVERVNNDGSILVSEMNYSAAPGILTYRTVPAYQVNNYRYIH
                     "
     gene            complement(1341..1610)
                     /locus_tag="PPY92_002148"
     CDS             complement(1341..1610)
                     /locus_tag="PPY92_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626131.1"
                     /translation="MAKRSKSQRLSSLLNVAGFIVDGYNGYKYHAKNKKLVYLSLGLS
                     TVGTVLDFYISIKSPRKFKKAVAVVTLITNGARLFTSIRKVKHEY"
     gene            1759..2154
                     /locus_tag="PPY92_002149"
     CDS             1759..2154
                     /locus_tag="PPY92_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDJ2626132.1"
                     /translation="MIKCVCLVEETADKILLVQVRNREKYYFPGGKIEEGESQVHALL
                     REVKEELNLTLTMDEIEYIGTIVGPAYPQQDMLTELNGFRALTKIDWENVTINNEITD
                     IRWIDKDNDALIAPAVKVWIETYGGKHDK"
     gene            2144..2629
                     /locus_tag="PPY92_002150"
     CDS             2144..2629
                     /locus_tag="PPY92_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDJ2626133.1"
                     /translation="MTNNDTIMLRHYVPQDYSMLEAFQLSESDLKFVKTPEENITAAM
                     SDNERYPIVVMDGRQCVAFFTLHRGKGVAPFSDNQDAVFFRSFSVDQRYRNRGIGKVV
                     MEKLASFITSTFQDINEIVLTVNTDNPHAMALYRQQGYQYMGDSMFIGRPVHIMALTI
                     K"
     gene            complement(2798..3580)
                     /locus_tag="PPY92_002151"
     CDS             complement(2798..3580)
                     /locus_tag="PPY92_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDJ2626134.1"
                     /translation="MNAVFILALLLLFLMIIFGGKKGFISYLTLFLNFAILIISIVLI
                     IFGVPIYVVTFFFCIIIGACNLFVLNSYNVKTQAAFISTIVTTILLITLIYLSVYFGH
                     LQGFSTEQQDETYVYSMNIGIDMVQFMVFTIILAVIAAVIDLTITISSPIYELHQVNP
                     TLTQYELFQSGMRVGREILATSANTIYLAFFGGQLALFIWFFKLKYSFGHIINSKIFA
                     QEFISIILGGIAVAISIPITAWITAFLIKRPSRQRKNDESIN"
     gene            complement(3577..4695)
                     /locus_tag="PPY92_002152"
     CDS             complement(3577..4695)
                     /locus_tag="PPY92_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDJ2626135.1"
                     /translation="MFLKYRNLFTKPFNIILGLFCIAFIGAILFTFNNENFYNKPIGQ
                     IIDVKHVSSTPTKDAQNNRDIKYKNQLKVKILNGQFAGETTTINHQYVKSQADSEAFR
                     THEKVLLHISNKPSDAYIIEKKRDTLTVIITGLFLLTVLLVGRKVGLQSILSLILNSI
                     AILIAIYIHIQHSNINLFLLMTIAMICSTILTLLLVTGWHMRTLITIASTIIGTFLSI
                     GLTELIIYMTDGKGIKYETMNFLSLPPKDIFLASVLIGSLGAIMDVAITIASGMHEIL
                     QRTPHISMRRWALAGRNIGQDIMGTMTNILLFSYLSGALPMFLIFLKNANTVTYTISM
                     NWSLEIARALTGGIGIVLTIPITILFMEIFETLRRAKQ"
     gene            complement(4810..5694)
                     /locus_tag="PPY92_002153"
     CDS             complement(4810..5694)
                     /locus_tag="PPY92_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDJ2626136.1"
                     /translation="MEIKQLRYFIEVAKREHISETALELNIAQSAISRQITLLEQELN
                     VSLFKKQGRNITLTSEGKLLFNEALRIIEHLDSTIEQFQSHGLTKNKSIYIGYDESDV
                     SHMLLPLIQTFHLQNDTHVIPSLLDHDTIINSVLNGNIDIGFTELTPEIRKHKQLHML
                     PLFEEHYHLYAPSDDPITMATHPPLIQFEHSHIYCLAPFAETVKKQLRKITKSDVYTI
                     SSQPLAQYLLRQKEGYIISSQNIHLPESKSWIDIKLDHTELKRTICAITKEPYTKSDI
                     GILLTLIQQLMTKTSTFH"
BASE COUNT         1980 a          898 c         1038 g         1874 t
ORIGIN      
        1 taaaatttga tgatacagta tatgattttt ttgtaatcat aatgtcatca aacatcaacc
       61 tattatacat aataaaatcg tataatgatg tagtattcat aaattcggat aaaagaatgt
      121 taggaaagtt aagcaagagg aggattttaa agtgcaaaaa aaagtaattg cagctattat
      181 tgggacaagc gcgattagcg ctgttgcggc aactcaagca aatgcggcta caactcacac
      241 agtaaaaccg ggtgaatcag tgtgggcaat ttcaaataag tatgggattt cgattgctaa
      301 attaaagtca ttaaacaatt taacatctaa tctaattttc ccaaaccaag tactaaaagt
      361 atctggctca agtaattcta cgagtaatag tagccgtcca tcaacgaact caggtggcgg
      421 atcatactac acagtacaag caggcgactc attatcatta atcgcatcaa aatatggtac
      481 aacttaccaa aacattatgc gacttaatgg tttaaataat ttctttattt atccaggtca
      541 aaaattaaaa gtatcaggta ctgctagctc aagtaacgct gcgagcaata gtagccgtcc
      601 atcaacgaac tcaggcggtg gatcatacta cacagtacaa gcaggtgact cattgtcatt
      661 aatcgcatca aaatatggta caacttatca aaaaattatg agcttaaatg gcttaaataa
      721 tttctttatt tatccgggtc aaaaattgaa agtaactggt aatgcatcta cgaactcagg
      781 atctgcaaca actacgaata gaggttacaa tacaccagta ttcagtcacc aaaacttata
      841 tacatggggt caatgtacat atcatgtatt taatcgtcgt gctgaaattg gtaaaggtat
      901 tagtacttat tggtggaatg ctaataactg ggataacgca gcggcagcag atggttacac
      961 tatcgacaat agacctactg taggttctat cgctcaaaca gatgtaggtt attatggtca
     1021 tgttatgttt gtagaacgtg taaataacga tggtagtatt ttagtttcag aaatgaacta
     1081 ttcagctgca ccaggtattt taacttacag aacggtacca gcttaccaag taaataatta
     1141 tagatatatt cactaaagtc ttacgtatat aaatatataa tgaattccta ttacatttca
     1201 caagctgaaa agttggattg aatcctaaac tttatggcgg ggctatcaaa gtcgtgattg
     1261 ttgtaatagg aatttttgta taagaaaaag gattggtcga atgaacttca tgttctatgt
     1321 tcgaccaatc cttttctgaa ttagtattca tgctttactt tgcgaatgct tgtaaataat
     1381 ctagcaccgt ttgttattaa agtaacaact gccactgctt ttttgaactt acgtggtgac
     1441 ttaattgaaa tgtaaaagtc taacacggtt cctacagtgc ttaaacctaa tgaaagatat
     1501 actaattttt tatttttagc atgatattta tagccattgt agccgtcgac tatgaaacct
     1561 gcgacattta gtaaacttga taaacgttgt gatttggaac gttttgccat aataataatt
     1621 ccccctagta tcttctgttt atatgaaaag ttgggtgcgc tgaattgcta acgttttgcg
     1681 ctataactac tcatatatga taacataatt gtacagtata atttgaaaaa ttgatttcac
     1741 aaagttgggg tgtcaaagat gattaaatgt gtctgtttag ttgaagaaac agctgataaa
     1801 atattgcttg ttcaagtaag gaatcgcgaa aagtattatt tcccaggtgg taaaatagaa
     1861 gaaggagaat cacaagtaca cgcgctgtta agagaagtaa aagaagaatt aaatttaaca
     1921 ttaacaatgg atgaaattga atatatcggg acaattgtag gtcctgcata tccacaacag
     1981 gatatgttaa ctgagttaaa tggatttcgc gcattaacca aaatcgattg ggaaaacgta
     2041 actatcaata atgaaattac ggatatacgc tggattgata aagataatga tgcgttgatt
     2101 gcgcctgctg tcaaagtttg gattgaaacg tatggtggta aacatgacaa ataatgacac
     2161 catcatgtta cgacattatg tcccacaaga ttattcgatg ttagaagctt ttcaattaag
     2221 tgaaagtgat ttgaagtttg ttaaaacgcc agaggaaaat attacagctg caatgtctga
     2281 taatgaaagg tatcccatcg ttgtaatgga tggcaggcaa tgtgtggcct tttttacatt
     2341 acatcgtgga aaaggggtcg caccatttag cgataaccaa gatgcagtat ttttcaggtc
     2401 atttagtgtt gatcaacgtt atcgtaatag aggaataggt aaagtggtaa tggaaaaatt
     2461 ggcgtcattt atcacttcaa catttcagga tattaatgag attgtgttaa cggttaatac
     2521 tgacaatcca catgccatgg cactttatcg ccaacaagga tatcaatata tgggagatag
     2581 tatgttcatc ggaagacctg ttcatattat ggcgttaact ataaaataaa ttaaatttaa
     2641 aagcatcttt actcatcgtc gaccacaaca attaatgatg aataaaggtg ctttttgtta
     2701 tagatcaccg gacaatttaa tatagtaaaa agcgacctag tgaacaattg acatatatcc
     2761 acaggtcgct taacttaagt tatattgcta gttgcgatta attgatagac tcatcatttt
     2821 tgcgctgtcg agatggtctt tttattaaaa atgccgtaat ccaagccgta atcggaatac
     2881 tgattgcaac ggcaataccg cctaaaataa tagaaataaa ttcttgggca aatattttcg
     2941 agtttataat atgaccaaat gaatatttaa gtttgaaaaa ccaaataaat aaagcaagtt
     3001 ggccaccaaa aaaggcaagg taaatcgtgt tcgcagatgt cgctaaaatt tctctaccaa
     3061 cacgcatgcc agattggaat aattcgtatt gcgtaagtgt tggatttact tgatgcaatt
     3121 cataaatggg tgaactaatg gtaatcgtta aatctatcac agctgcaata acagcaagaa
     3181 taatagtgaa caccataaat tgaaccatat caatgccaat attcattgaa tacacatatg
     3241 tttcatcttg ttgttcggtt gaaaagcctt gtagatgacc gaagtagaca gataaataaa
     3301 tgagtgtaat caacaatatt gttgtaacga tagtgctgat aaatgcagct tgtgttttaa
     3361 cattgtaact attgagtacg aataaattac aagcgccaat aataatgcag aaaaagaatg
     3421 tgacgacata aatcggtacg ccaaaaataa tcaatacaat actaataatt aaaatagcga
     3481 aatttaaaaa tagggttaaa taagagatga atcccttttt acctccgaaa attatcatca
     3541 gaaagaggag caataacgcc aatataaata cagcattcat tgtttcgccc tccttaatgt
     3601 ttcaaatatt tccataaaca atattgtgat aggaattgta agtacgatac ctataccgcc
     3661 tgttagtgcg cgcgcaattt ctaacgacca attcatcgaa atagtataag tcacagtatt
     3721 tgcatttttt aaaaagatta aaaacatagg tagtgcaccg gataaatatg agaataataa
     3781 gatgttagtc attgttccca taatatcttg gccgatgttt cgcccagcaa gcgcccatct
     3841 cctcattgaa atgtgtggcg tacgctgtaa aatttcatgc ataccactag caattgtaat
     3901 tgcaacatcc ataatagcgc caagtgaacc tattaacact gaggctagga agatatcttt
     3961 cggtggtaat gataaaaagt tcatcgtttc atatttaatg cctttaccat ctgtcatata
     4021 tatgattaat tctgttaaac ctatactcaa aaaagttccg ataattgtac tggctatggt
     4081 aatgagtgta cgcatatgcc agcctgtaac gagcaataaa gtgagtattg ttgaacagat
     4141 catggcaatg gtcatgagta agaataaatt aatattgcta tgttgaatat gaatgtaaat
     4201 tgcgattaat atggcaatag aattcaagat taacgataaa atcgattgca gtccgacttt
     4261 gcgaccaacc aataatacag ttaataagaa caaaccagtg atgataaccg ttaaggtatc
     4321 acgcttcttt tctataatat aagcatcact cggcttgtta gaaatatgta ataatacttt
     4381 ttcgtgtgtg cgaaatgcct cagaatctgc ttgcgatttg acgtactgat gattaatcgt
     4441 cgtcgtttct ccagcaaatt gaccatttaa tattttgact tttaattgat ttttatattt
     4501 aatatcacga ttattttgtg catcttttgt aggtgtcgaa gaaacatgtt tgacatctat
     4561 aatttgacca attggtttgt tgtaaaagtt ctcattattg aatgtaaata aaatagcacc
     4621 aatgaatgcg atgcagaaca aacctaaaat tatattaaat ggctttgtaa ataaatttct
     4681 atatttcaaa aacaaaaccc caattctatg aatgaattaa tatggtgatt atacgccctt
     4741 aattttttat tttcaaagat attactgcta agtgtaaaac gaaaatcatc attgatagca
     4801 tcgaattact taatggaatg tagacgtttt agtcattaat tgctgaataa gtgttaataa
     4861 tatgccaata tcactctttg tataaggctc ctttgtaata gcacatatcg ttctttttaa
     4921 ttcagtatga tctaatttta tatctatcca tgatttagat tctggtaaat gtatattttg
     4981 tgatgaaatg atgtaacctt ctttttgacg aaggagatac tgcgcaagtg gttggctact
     5041 gattgtgtat acatctgatt tagtaatctt gcgcaattgt ttttttacag tttcggcaaa
     5101 tggtgccaag caataaatat gactatgctc aaactgaatt aatggtgggt gtgtcgccat
     5161 cgtaattgga tcgtctgaag gcgcatataa atgatagtgc tcttcgaata aaggtagcat
     5221 atgtaattgt ttgtgtttac gtatttctgg tgtaagttcc gtgaaaccaa tgtctatatt
     5281 cccatttaat acgctattta taatcgtgtc atgatctaat aagctcggta tgacatgtgt
     5341 atcattttgt aaatgaaacg tttggataag tggtagtaac atgtgggata cgtcactctc
     5401 atcatagcca atgtagatac ttttattttt agttaatcca tggctttgaa attgttcaat
     5461 cgtgctatct aaatgttcaa taatacgcag agcttcatta aataataatt tcccttcaga
     5521 tgtgagcgta atattgcgtc cttgcttttt aaataaagac acattaagtt cttgttctaa
     5581 taatgtaatt tgacggctta tcgctgattg agcaatgttt agttcaagtg ctgtttcgga
     5641 gatatgttct cttttagcga cctcgataaa atatcttaat tgtttaattt ccatagcgat
     5701 ataggcacct ccgaaaatga gtgttttgta actattatag caatattatt gataaatgtt
     5761 ctatttttta gatgaatatc ttctatttta
//
LOCUS       DANBWH010000167         5789 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.173, whole genome shotgun sequence.
ACCESSION   DANBWH010000167 DANBWH010000000
VERSION     DANBWH010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5789
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.173"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            111..1940
                     /locus_tag="PPY92_002154"
     CDS             111..1940
                     /locus_tag="PPY92_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDJ2626137.1"
                     /translation="MFNQFKRLIIGQPKKNRELKDEKISKFKGLAILSSDALSSVAYG
                     PEQILITLSVVGAVATWYTLPIAGAVLILLAALIMSYRQIIYAYPKGGGAYMVSKTNL
                     GEKWGLLAGGSLLVDYILTVAVSISSGADAFVAAFPSLYGHKVLIACLLVLFILILNL
                     RGLTESATVLSYPVYLFIIGLVILIFIGTFRVATGDIQPHMHASVGTAVPGVTLFLLL
                     KAFSSGASSLTGVEAISNAVTNFREPSANNAVKTLIAMGSILAFLLVGIVGLAYVYGI
                     MPQTETTVLSQLAMQIFGDNAAFYFVQATTVMILVLAANTGFTAFPMLAASMSKDKYM
                     PRMFTVRGDRLGYSNSIIILGVLAIILIIVFDGMTEDLIPLYAVGVFIPFTLAQFGMV
                     IKWIHERPKNWLSKLSVNLLGGIVTFIVFMILLITKFSQVWPILIFLPFVVIFFLKIN
                     KHYRDIAEQLRSDIDVLNVDVVDRNLAIVPITSITTAVDKSIYYAQMLANNDVIAVHV
                     SFGDEDEKAFQEKWKRHFPDVRLVILHSEYRSIIRPISRFIDKINRKANDQNYMITVV
                     IPEFITKKRWHNLLHNQTSLRMKLYLIYQKNVNVCTIPFKLKK"
     gene            complement(2081..4159)
                     /locus_tag="PPY92_002155"
     CDS             complement(2081..4159)
                     /locus_tag="PPY92_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDJ2626138.1"
                     /translation="MALLEAFLIFIFAVIISSVINNRFPQIPTAFIQIALGVVIFIIP
                     IQVDFQFNSEVFMFAVIAPLLFVEGTHVSRTKLLEYRKPILLMSMALVFATVVGVGYF
                     IHWIWPALPMPAAFAIAAILCPTDAVAVSAITRGKLLPKGSMTILEGESLLNDAAGII
                     SFKIAVTALVTGTFSLFQAVEQFIISTILGVLIGAIIGFVVVRIRIDLTANKGLKDNN
                     TLTFIQLLTPFVVYFLAEEVHASGIIAVVIAGLIHGLERDRLIRAQTELQMNYHQIWN
                     TFSYALNGFVFVVLGFMIPTVVIDIFQTEPDNFSFLIVITILIAIAIYACRFVWVYFW
                     YKDFYFPKNIQSYLDEEHDSHETPPSRVRYAFIMTMCGIHGTISLSMALTLPFIITKG
                     QAFEYRNDLLFIASFMVLISLILAQIVLPLITPSAEDTTFKGMTYQSAKIFIVQKVIQ
                     HFKNESKKDKNDTNYRPVLNQYYGELLFLLNSEPNNQNTKELKRLEDIAKVIETSTLE
                     RLIDKGKATYQDINNYRNIVELTETHRTASFVEKFANFFKMLWLRLKAMKHYKALNKE
                     SKKQEFENSFKDVQKIMRIVNHNIILRLKEEQNSTNVLEVSLVINHYYDMSRSLKWRA
                     QRRKERQENSNQIIPQAMFHNHKLEALYLQRHLLDELIRKNKINNIVAAQIRENINYN
                     EIVLSLQSKH"
     gene            complement(4298..5707)
                     /locus_tag="PPY92_002156"
     CDS             complement(4298..5707)
                     /locus_tag="PPY92_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDJ2626139.1"
                     /translation="MARKLHRELNNRHIQLIAIGGAIGTGLFLGSGQTISLTGPSLLF
                     TYMIIGVVLFAFMRALGELLLSNTRFNSFVDIANEYLGPFGGFVIGWTYWLCWIVSSM
                     SDLTAMGQYFAFWYPQVPNWITVLFIVLILISFNLLGARLFGELEFWFSIIKVVTIIA
                     MVIVGLVLIFFSFKTHYGHASFTNLISHGGMFPGGTFGFLMSFQIAVYSFIGIELIGV
                     TAGETKDPEKTLPKAINNVPIRILLFYIGGLLVIMSVIPWNDIDPNSSPFVKLFTLIG
                     VPFAAGVVNFVVLTAAASATNSGIYSNSRILFGLSQQGLGPKVLNKTNSHGVPYLSML
                     VSSIALLIAALLNYIFPNAIQLFIYVTTLSTVLFLVVWAMIIVAYLMYLKKHPEAHKN
                     SKFKLIGGKPIAYIILAFFFFVFILLFFSDETRAAIYISPFWFIFLFFFYKKYKTNAE
                     KLAYEQRQNDSGHFRYDNQ"
BASE COUNT         1953 a          905 c         1013 g         1918 t
ORIGIN      
        1 tgttaatagg tgtataatta cataaaaaat attaattaac tctctatact ttttggaaag
       61 ataggcgtat agtgatatcc atcaaaaact ataaaaagga ataggagatt atgttcaatc
      121 aatttaaaag acttattata gggcaaccta aaaaaaacag agaactaaaa gacgaaaaga
      181 tatcgaagtt taaagggtta gcgattcttt cgtcggatgc attatcttca gtggcttacg
      241 gtcccgaaca aatactgatt acattatcag tagtgggtgc agttgcaact tggtatactt
      301 taccgattgc aggtgctgtt ctgatcttat tagctgcttt aattatgtct tataggcaaa
      361 ttatttatgc atatcctaaa ggtggcggcg cgtatatggt gtccaagacc aatttaggag
      421 aaaaatgggg attgcttgca ggaggctcat tattagtcga ttacatactg actgtcgctg
      481 ttagtatatc atctggtgct gatgcatttg ttgctgcatt cccaagttta tatgggcata
      541 aagtactgat tgcatgttta cttgtacttt ttattttaat attaaattta cgtggtttaa
      601 ctgaatctgc caccgtatta tcctatccag tatatctttt cattataggg ttagtgatat
      661 taatatttat aggtactttc cgtgtggcga caggagatat tcaaccacat atgcatgcat
      721 cagtaggaac tgcggttcct ggagtaacat tattcttgct attaaaagct ttttcatcag
      781 gggcttcatc attaacaggt gttgaagcta tttcaaatgc ggtaaccaat tttagagaac
      841 caagtgctaa taatgctgta aaaacgttaa ttgctatggg ttcaatttta gcgtttttat
      901 tagttggtat cgttggttta gcatatgttt atggaattat gccacaaacg gaaacgacag
      961 ttttatcaca attagctatg caaatatttg gtgataatgc tgccttctat tttgtacagg
     1021 caacgaccgt gatgattttg gtattagcag ccaacactgg atttacagct tttccgatgt
     1081 tagcggcttc aatgtctaaa gataaatata tgcctagaat gtttactgtt agaggtgatc
     1141 gcttaggcta ctctaactca attattattt taggtgtatt agccattatt ttaattattg
     1201 tatttgatgg tatgactgaa gatttaattc cattgtatgc tgttggtgta tttattccat
     1261 ttacactagc acaattcggt atggttatta aatggattca tgaacgtcct aagaattggt
     1321 taagtaagtt gtctgtgaac ttactaggtg gtatcgtgac atttattgtt tttatgattt
     1381 tactcataac taaatttagc caagtatggc cgatacttat tttcttgccg ttcgtggtga
     1441 ttttcttctt gaaaattaac aaacattatc gagatatcgc agaacaatta cgttctgaca
     1501 ttgatgttct taatgtggac gtggtagatc gtaatttagc aattgtgccg ataacaagta
     1561 ttacaacagc agttgataag tctatttatt acgcacaaat gcttgcgaat aatgatgtca
     1621 tagcagtaca tgtatcattt ggagatgaag atgaaaaagc attccaagag aaatggaaac
     1681 gtcatttccc agatgtaaga ttagttattt tacattcgga atatcgcagt attatacgac
     1741 caatttcacg ttttatcgat aagattaatc gtaaagcaaa cgatcaaaac tatatgatta
     1801 cagtagtgat accagaattt attactaaga aacgttggca taatttatta cacaatcaaa
     1861 ctagtttgcg tatgaaattg tatttaattt atcaaaagaa tgttaacgta tgtacaattc
     1921 catttaaact taaaaaatag attctataat caaatcggat tgatgaaagg ttatttactt
     1981 ttcatcaatc cgattttttg atgttgaata taaaaacccc gtacatatga gatgataatg
     2041 actttgtctc atatgtatgg ggggtattgt tatgtcttgc ttaatgtttg gactgtaaag
     2101 acaagacaat ttcgttgtaa ttgatatttt ctcgaatttg agctgcaacg atattgttga
     2161 ttttattttt gcgaattaat tcatctaaaa ggtgacgttg taagtacaat gcttccaatt
     2221 tgtggttatg gaacatagct tgcggtatga tttgattgct gttttcttga cgttcttttc
     2281 gacgttgtgc acgccacttt aatgagcgac tcatatcata gtaatgatta atgactaagc
     2341 taacctcaag tacatttgta ctattttgtt cttcttttaa gcgtaaaata atattgtgat
     2401 tcacaatacg cataattttt tgaacatctt tgaatgaatt ttcaaattct tgcttcttag
     2461 attctttgtt taaggctttg tagtgcttca tcgctttaag tctaagccat aacatcttga
     2521 agaagtttgc aaatttttca acaaatgatg cagtacggtg tgtctctgtt aattcgacaa
     2581 tattgcggta attattaata tcctgatacg ttgccttacc tttatcaatt aaacgctcaa
     2641 gtgtagatgt ttcgattact tttgcaatat cttctaaacg tttgagttct tttgtatttt
     2701 gattattagg ttctgaattt aataaaaata acaattctcc atagtattgg tttaatactg
     2761 ggcgataatt tgtatcgttt ttgtcttttt tactttcgtt tttaaaatgc tgtatcactt
     2821 tttgaacaat gaaaattttg gcagattgat aagtcatacc tttaaaagta gtatcttcgg
     2881 cagatggtgt aattaaaggt aaaacaattt gcgctaagat taaactaatt aataccatga
     2941 aagatgcaat aaacaataaa tcattacggt attcgaatgc ttgtcctttt gtaatgataa
     3001 atggtaatgt aagtgccatt gaaagtgaaa ttgtaccgtg aataccacac atcgtcataa
     3061 taaatgcgta acgcacacga gaaggtggtg tttcatgtga atcatgttct tcgtctagat
     3121 aagattgtat atttttcggg aaataaaaat ctttgtacca gaaataaacc caaacaaatc
     3181 gacatgcata aattgcaata gcgattaata tagtgatcac tattaaaaat gaaaagttgt
     3241 caggctctgt ttggaaaata tctatgacta cagtaggaat cataaaacct aatacaacaa
     3301 atacaaaacc atttaaggca taactaaatg tgttccaaat ttgatggtaa ttcatttgta
     3361 gttccgtttg tgctctaatt aaacggtcgc gttctaaacc atggattaga cctgcgatta
     3421 caactgcaat gatacctgaa gcatgaactt cttctgctaa aaagtatacg acaaaaggag
     3481 ttaataattg aataaaagtt aaggtattgt tatcttttaa acctttatta gcggttaagt
     3541 ctatacgtat tctaacgaca acgaatccaa taattgcacc aataagtaca cctagtattg
     3601 ttgaaatgat aaattgttca acagcttgga ataatgaaaa ggtaccagtt actaatgcag
     3661 taacagcaat tttaaatgaa atgatacctg ctgcatcatt gagtaaagat tcaccttcta
     3721 aaatcgtcat agaaccttta ggtaataatt ttccgcgtgt aatagcagat actgctactg
     3781 catcagtagg acataaaatt gctgctattg caaaagcggc tggcattggt aaggcaggcc
     3841 aaatccaatg tataaaatag ccaacaccga ctacagttgc aaacactagt gccattgaca
     3901 ttaatagtat aggtttacga tattctaata attttgttcg agagacgtgg gtaccttcta
     3961 caaaaagtag tggcgcgata acggcaaaca taaacacttc agaattgaat tggaaatcaa
     4021 cttgtattgg aataatgaaa ataacgacac ctaatgcaat ttgaataaag gcagtaggaa
     4081 tttgtgggaa tcgattattg ataaccgaac taataatcac agcaaaaata aaaattaaaa
     4141 atgcttctaa tagtgccata caatactcct caaaatttta atagttaata ttttatcact
     4201 tttaaggcat aatgacatag atatattgat aaaatgaagt tattttcaaa aaaaactcta
     4261 gtatcggttg aactgatact agagcgagat gtttaaatta ttgattgtca tatctgaaat
     4321 gaccgctgtc attttgtcgt tgttcatacg cgagcttttc agcattcgtt ttgtattttt
     4381 tataaaagaa aaataaaaat ataaaccaga atggtgaaat ataaatagct gctcttgttt
     4441 cgtcactaaa gaataataaa atgaatacaa agaagaagaa cgctagaata atgtaagcaa
     4501 taggcttacc accaatcaac ttaaatttac tgtttttatg tgcctcagga tgctttttca
     4561 aatacattag ataagcgaca ataatcattg cccaaacaac taaaaacaac acagttgata
     4621 acgttgtaac gtatatgaat agttgaattg cattagggaa aatgtagttt aacaaggctg
     4681 ctataagtaa tgcaattgat gaaactaaca ttgataaata aggcacgcca tgactattcg
     4741 ttttatttaa aactttagga cctaaccctt gttgtgacag tccgaataag atacgactat
     4801 tcgaatagat accactattt gtagcagagg ccgcggcagt tagcacgaca aagttaacga
     4861 cgcctgctgc aaatggcacg ccgattaatg taaagagttt aacgaaaggg ctactatttg
     4921 gatcgatatc attccaaggt atgactgaca taattactaa tagaccaccg atatagaata
     4981 ataaaatacg gataggtaca ttattaattg ctttcggtaa ggttttttca ggatctttcg
     5041 tttcaccagc agttacacct ataagttcaa taccaatgaa tgaatataca gcaatttgga
     5101 atgacattag gaaaccaaat gttcctccag ggaacatgcc gccgtgactg attaagtttg
     5161 tgaatgatgc atgtccataa tgtgttttaa atgagaaaaa gattaataca agaccaacga
     5221 taaccatcgc aataattgtg acaactttaa taatagagaa ccaaaactcc agttcaccaa
     5281 ataatctggc acctaataag ttgaagctaa tcaagattaa aacaataaat agcacggtaa
     5341 tccaatttgg gacttgtgga taccaaaatg caaagtattg tcccatcgca gttaggtctg
     5401 acatacttga tacaatccaa cataaccagt aagtccagcc aatgacaaag ccaccaaaag
     5461 ggcctaaata ttcatttgca atatcaacaa atgaattaaa tcttgtattg ctcaacaaca
     5521 attcgcctaa tgcgcgcata aaagcgaata gtacaacccc aataatcatg tatgtgaata
     5581 acagtgatgg accagttaaa gatattgttt gacctgatcc taggaataac ccagttccaa
     5641 ttgcgccccc aattgctatc aattggatgt gtctgttatt caactctcta tgcaattttc
     5701 ttgccataat tttcctcctc aggataaagc aaataaaaaa gttgtgtgta agcgttttct
     5761 taattataaa tactaaagaa atccattta
//
LOCUS       DANBWH010000168         5778 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.174, whole genome shotgun sequence.
ACCESSION   DANBWH010000168 DANBWH010000000
VERSION     DANBWH010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.174"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            13..993
                     /locus_tag="PPY92_002157"
     CDS             13..993
                     /locus_tag="PPY92_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDJ2626140.1"
                     /translation="MEDLERVLITGGAGFIGSHLVDDLQQDYDVYVLDNYRTGKRENI
                     KSLADDHVFELDIREYDAVEQIMKTYQFDYVIHLAALVSVAESVEKPILSQEINVVAT
                     LRLLEIIKKYNSHIKRFIFASSAAVYGDLPDLPKSDQSLILPLSPYAIDKYYGERTTL
                     NYCSLYNIPTAVVKFFNVFGPRQDPKSQYSGVISKMFDSFEHNKPFTFFGDGLQTRDF
                     VYVYDVVQSVRLIMEHKDAIGHGYNIGTGTFTNLLEVYRIIGELYGKSVEHEFKEARK
                     GDIKHSYADISNLKALGFVPKYTVETGLKDYFNFEVDNIEEVTAKEVEMS"
     gene            956..1648
                     /locus_tag="PPY92_002158"
     CDS             956..1648
                     /locus_tag="PPY92_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDJ2626141.1"
                     /translation="MKKLQLKKWKCRENDIEAVHNNKGYAYQRKLDALEEVRKGYYPI
                     KRAIDLVLSIVLLFLTFPIMFIFAIAIVIDSPGNPIYSQVRVGKMGKLIKIYKLRSMC
                     KNAEKNGAQWADKDDDRITNVGKFIRKTRIDELPQLINVVKGEMSFIGPRPERPEFVE
                     LFSSEVIGFEQRCLVTPGLTGLAQIQGGYDLTPQQKLKYDMKYIHKGSLMMELYISIR
                     TLMVVITGEGSR"
     gene            1858..3024
                     /locus_tag="PPY92_002159"
     CDS             1858..3024
                     /locus_tag="PPY92_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDJ2626142.1"
                     /translation="MKIIYCITKADNGGAQTHLIQLANHFCVHHDVYVIVGNHGPMIE
                     QLDARVNVIILEHLVGPIDFKQDILAVKVLAQLFSKIKPDVIHLHSSKAGTVGRIAKF
                     ISKSKDTRVVFTAHGWAFTEGVKPAKKFLYLVIEKLMSCITDSIICVSDFDKQLALKY
                     RFNRLKLTTIHNGIADVPAVKQTLKSQSHNNIGEVVGMLPNKQDLQINAPTKHQFVMI
                     ARFAYPKLPQNLIAAIEILKLHNSNHAHFTFIGDGPTLNDCQQQVVQAGLENDVTFLG
                     NVINASHLLSQYDTFILISKHEGLPISIIEAMATGLPVIASHVGGISELVADNGICMM
                     NNQPETIAKVLEKYLIDSDYIKMSNQSRKRYLECFTEEKMIKEVEDVYNGKSTQ"
     gene            3005..4243
                     /locus_tag="PPY92_002160"
     CDS             3005..4243
                     /locus_tag="PPY92_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDJ2626143.1"
                     /translation="MENQHNSKLLTLLLIGLAVFIQQSSVIAGVNVSIADFITLLILV
                     YLLFFANHLLKANHFLQFFIILYTYRMIITLCLLFFDDLIFITVKEVLASTVKYAFVV
                     IYFYLGMIIFKLGNSKKVIVTSYIISSVTIGLFCIIAGLNKSPLLMKLLYFDEIRSKG
                     LMNDPNYFAMTQIITLVLAYKYIHNYIFKVLACGILLWSLTTTGSKTAFIILIVLAIY
                     FFIKKLFSRNAVSVVSMLVIMLILLCFTFYNINYYLFQLSDLDAVPSLDRMASIFEEG
                     FASLNDSGSERSVVWINAILVIKYTLGFGVGLVDYVHIGSQINGILLVAHNTYLQIFA
                     EWGILFGALFIIFMLYLLFELFRFNISGKNVTAIVVMLTMLIYFLTVSFNNSRYVAFI
                     LGIIVFIVQYEKMERDRNEE"
     gene            4233..5663
                     /locus_tag="PPY92_002161"
     CDS             4233..5663
                     /locus_tag="PPY92_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HDJ2626144.1"
                     /translation="MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVS
                     FSFNIVQYFLMIASVGVQLYFNRVIAKSVNDKRQLSQQFWDIFVSKLFLALTVFAMYM
                     VVITIFIDDYYLIFLLQGIYIIGAALDISWFYAGTEKFKIPSLSNIVASGIVLSVVVI
                     FVKDQSDLSLYVFTIAIVTVLNQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPN
                     GQLNLYTSISCVVLGLVGTYQQVGIFSNAFNILTVAIIMINTFDLVMIPRITKMSIQQ
                     SHSLTKTLANNMNIQLILTIPMVFGLIAIMPSFYLWFFGEEFASTVPLMTILAILVLI
                     IPLNMLISRQYLLIVNKIRLYNASITIGAVMNLVLCLVLIYFYGIYGAAIARLITEFI
                     LLIWRFVDITKINVKLNIVSTIQCVIAAVMMFIVLGVVNHYLPPTMYATLLLIAIGIV
                     VYLLLMMTMKNQYVWQILRHLRHKTI"
BASE COUNT         1867 a          791 c         1061 g         2059 t
ORIGIN      
        1 tgtataaatg aggtggaaga tttggaaaga gttttgataa ctggtggggc tggttttatt
       61 gggtcgcatt tagtagatga tttacaacaa gattatgatg tttatgttct agataactat
      121 agaacaggta aacgagaaaa tattaaaagt ttggctgacg atcatgtgtt tgaattagat
      181 attcgtgaat atgatgcagt tgaacaaatc atgaagacat atcaatttga ttatgttatt
      241 catttagcag cattagttag tgttgctgag tcggttgaga aacctatctt atctcaagaa
      301 ataaacgtcg tagcaacatt aagattgtta gaaatcatta aaaaatataa tagtcatata
      361 aaacgtttta tctttgcttc gtcagcagct gtttatggtg atcttcctga tttgcctaaa
      421 agtgatcaat cattaatctt accattatca ccatatgcaa tagataaata ttacggcgaa
      481 cggacgacat taaattattg ctcgttatat aacataccaa cagcggttgt taaatttttt
      541 aatgtatttg ggccaagaca ggatcctaag tcacaatatt caggtgttat ttcaaagatg
      601 ttcgattcat ttgagcataa caagccattt acattttttg gtgacggact gcaaactaga
      661 gattttgtat atgtatatga tgttgttcaa tctgtacgct taattatgga acacaaagat
      721 gcaattggac acggttataa cattggtaca ggcactttta ctaatttatt agaggtttat
      781 cgtattattg gtgaattata tggaaaatca gtcgagcatg aatttaaaga agcacgaaaa
      841 ggagatatta agcattctta tgcagatatt tctaacttaa aggcattagg atttgttcct
      901 aaatatacag tagaaacagg tttaaaggat tactttaatt ttgaggtaga taatattgaa
      961 gaagttacag ctaaagaagt ggaaatgtcg tgaaaatgac attgaagctg tccataataa
     1021 taagggttat gcctatcaaa gaaaattaga tgcactcgaa gaagtgagaa aaggctatta
     1081 cccaattaaa cgtgcgattg acttagtatt aagtatcgtt ttattatttt taacatttcc
     1141 gattatgttc atattcgcca ttgctatcgt catagattcg ccaggaaacc ctatttatag
     1201 tcaggttaga gttgggaaga tgggtaaatt aattaaaata tacaaattac gttcgatgtg
     1261 taaaaacgca gagaaaaatg gtgcgcaatg ggctgataaa gatgatgatc gtataacaaa
     1321 tgtcgggaag tttattcgta aaacacgcat tgatgaatta ccacaactaa ttaatgttgt
     1381 taaaggggaa atgagtttta ttggaccacg cccggaacgt ccggaatttg tagaattatt
     1441 tagttcagaa gtgataggtt tcgagcaaag atgtcttgtt acaccagggt taacaggact
     1501 tgcgcaaatt caaggtggat atgacttaac accgcaacaa aaactgaaat atgacatgaa
     1561 atatatacat aaaggtagtt taatgatgga actatatata tcaattagaa cattgatggt
     1621 tgttattaca ggggaaggct caaggtagtc ttaatttact taataagttc aaataaaagt
     1681 tatattttaa agattgtgac caattgttac agtataacga ggaatccctt gagacagtat
     1741 caaatggcat taagaaatat gtgccatcat tgatttgcat ggctataaat actattcatc
     1801 tgatgagata gccatgttaa gaaattgaaa gtatagcatt aaaggggttt gtaacagttg
     1861 aaaattatat attgtattac taaagcagac aatggtggtg cacaaacaca tctcattcaa
     1921 ctcgccaacc atttttgcgt acaccatgat gtttatgtca ttgtaggcaa tcatggacca
     1981 atgattgaac aactagatgc aagagttaat gtaattattc tcgaacattt agtaggtcca
     2041 attgacttta aacaagatat tttagctgtc aaagtgttag cacagttatt ctcgaaaatt
     2101 aagcctgatg ttatccattt acattcttcc aaagctggaa cggtcggacg aattgcgaag
     2161 ttcatttcga aatcgaaaga cacacgtgta gtttttactg cgcatggatg ggcttttaca
     2221 gagggtgtta aaccagctaa aaaatttcta tatctagtta tcgaaaaatt aatgtcatgt
     2281 attacagata gcattatttg tgtttcagat ttcgataaac agttagcgtt aaaatatcga
     2341 tttaatcgat tgaaattaac cacaatacat aatggtattg cagatgttcc cgctgttaag
     2401 caaacgctaa aaagccaatc acataacaat attggcgaag tagttggaat gttgcctaat
     2461 aaacaagatt tacagattaa tgccccgaca aagcatcaat ttgttatgat tgcaagattt
     2521 gcttatccaa aattgccaca aaatctaatc gcggcaatag agatattgaa attacataac
     2581 agtaatcatg cgcattttac atttataggc gatggaccta cattaaatga ttgtcagcaa
     2641 caagttgtac aagctgggtt agaaaatgat gtcacatttt tgggcaatgt cattaatgcg
     2701 agtcatttat tatcacaata cgatacgttt attttaataa gtaagcatga aggtttgcca
     2761 attagcatta tagaagctat ggctacaggt ttgcctgtta tagccagtca tgttggcggt
     2821 atttcagaat tagtagctga taatggtata tgtatgatga acaaccaacc cgaaactatt
     2881 gctaaagtcc tggaaaaata tttaatagac agtgattaca tcaaaatgag taatcaatct
     2941 agaaaacgtt atttagaatg ttttactgag gagaaaatga ttaaagaagt ggaagacgtt
     3001 tataatggaa aatcaacaca atagtaaatt actaacattg ttacttatcg gtttagcggt
     3061 ttttattcag caatcttcgg ttattgccgg tgtgaatgtt tctatagctg actttatcac
     3121 attactaata ttagtttatt tactgttttt cgctaaccat ttattaaagg caaatcattt
     3181 tttacagttt ttcattattt tgtatacata tcgtatgatt attacgcttt gtttgctatt
     3241 ttttgatgat ttgatattta ttacggttaa ggaagttctt gcatctacag ttaaatatgc
     3301 atttgtagtc atttatttct atttagggat gatcatcttt aagttaggta atagcaaaaa
     3361 agtgatcgtt acctcttata ttataagcag tgtgactata ggtctatttt gtattatagc
     3421 tggtttgaac aagtcccctt tactaatgaa attgttatat tttgatgaaa tacgttcaaa
     3481 aggattaatg aatgacccta actatttcgc gatgacacag attattacat tggtacttgc
     3541 ttacaagtat attcataatt acatattcaa ggtccttgca tgtggtattt tgctatggtc
     3601 tttaactaca acggggtcta agactgcgtt tatcatatta atcgtcttag ccatttattt
     3661 ctttattaaa aagttattta gtagaaatgc ggtaagtgtt gtgagtatgt tagtgattat
     3721 gctgatatta ctttgtttta ccttttataa tatcaactac tatttattcc aattaagcga
     3781 ccttgatgcc gtaccgtcat tagatcgaat ggcgtctatt tttgaagagg gctttgcatc
     3841 attaaatgat agtgggtctg agcgaagtgt tgtatggata aatgccattt tagtaattaa
     3901 atatacacta ggttttggtg tcggattagt ggattatgta catattggct cgcaaattaa
     3961 tggtatttta cttgttgccc ataatacata tttgcagatc tttgcggaat ggggcatttt
     4021 attcggtgca ttatttatca tatttatgct ttatttactg tttgaattat ttagatttaa
     4081 catttctggg aaaaatgtaa cagcaattgt tgtaatgttg acgatgctga tttacttttt
     4141 aacagtatca tttaataact caagatatgt cgcttttatt ttaggaatta tcgtctttat
     4201 tgttcaatat gaaaagatgg aaagggatcg taatgaagag tgattcacta aaagaaaata
     4261 ttatttatca agggctatac caattgatta gaacgatgac accactgatt acaataccca
     4321 ttatttcacg tgcatttggt cccagtggtg tgggtattgt ttcattttct ttcaatatcg
     4381 tgcaatactt tttgatgatt gcaagtgttg gcgttcagtt atattttaat agagttatcg
     4441 cgaagtctgt taacgacaaa cggcaattgt ctcagcagtt ttgggatatc tttgtcagta
     4501 aattattttt agcgttaaca gtttttgcga tgtatatggt cgtaattact atatttattg
     4561 atgattacta tcttattttc ctactacaag gaatctatat tataggtgca gcactcgata
     4621 tttcatggtt ttatgctgga actgaaaagt ttaaaattcc tagcctcagt aatattgttg
     4681 cgtctggtat tgtattaagt gtagttgtta tttttgtcaa agatcaatca gatttatcat
     4741 tgtatgtatt tactattgct attgtgacgg tattaaacca attacctttg tttatctatt
     4801 taaaacgata cattagcttt gtttcggtta attggataca cgtctggcaa ttgtttcgtt
     4861 cgtcattagc atacttatta ccaaatggac agctcaactt atatactagt atttcttgcg
     4921 ttgttcttgg tttagtaggc acataccaac aagttggtat cttttctaac gcatttaata
     4981 ttttaacggt cgcaatcata atgattaata catttgatct tgtaatgatt ccgcgtatta
     5041 ccaaaatgtc tatccagcaa tcacatagtt taactaaaac gttagctaat aatatgaata
     5101 ttcaattgat attaacaata cctatggtct ttggtttaat tgcaattatg ccatcatttt
     5161 atttatggtt ctttggtgag gaattcgcat caactgtccc attgatgacc attttagcga
     5221 tacttgtatt aatcattcct ttaaatatgt tgataagcag gcaatattta ttaatagtga
     5281 ataaaataag attatataat gcgtcaatta ctattggtgc ggtaatgaat ttagtattat
     5341 gtcttgtttt gatatatttt tatggaattt acggtgcggc tattgcacgt ttaattacag
     5401 agtttatctt acttatttgg cgatttgttg atattactaa aatcaatgtg aagttgaata
     5461 ttgtaagtac gattcaatgt gtcattgctg ccgttatgat gtttattgtg cttggtgtgg
     5521 tcaatcatta tttgccccct acaatgtacg ctacgctgct attaattgcg attggtatag
     5581 tagtttatct tttattaatg atgactatga aaaatcaata cgtatggcaa atattgaggc
     5641 atcttcgaca taaaacaatt taagtatcgg taacgctata ctttagaaat ttaagattaa
     5701 gaagaaaagg caatttctta ttgaaaaatg gaagttgtct tttttaattc tctttaaaag
     5761 cgggaaacaa aagcagtt
//
LOCUS       DANBWH010000169         5773 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.175, whole genome shotgun sequence.
ACCESSION   DANBWH010000169 DANBWH010000000
VERSION     DANBWH010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5773
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.175"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..304)
                     /locus_tag="PPY92_002162"
     CDS             complement(<1..304)
                     /locus_tag="PPY92_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDJ2626145.1"
                     /translation="MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVI
                     SIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDILEDFKGVDKNDIEIRQQRIIL"
     gene            complement(222..521)
                     /locus_tag="PPY92_002163"
     CDS             complement(222..521)
                     /locus_tag="PPY92_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGE"
                     /protein_id="HDJ2626146.1"
                     /translation="MKSYKCKGSFLIDSMAGFLLIGLITLLLIPMMNQMQASINHKLQ
                     SIDASKVILTTVSKINKEELKKGVTIGKYDIKQSDQQICAISKNTTSYQKTCIQY"
     gene            complement(508..954)
                     /locus_tag="PPY92_002164"
     CDS             complement(508..954)
                     /locus_tag="PPY92_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDJ2626147.1"
                     /translation="MEKQLQIRKQSAFTMIEMLVVMMLISIFLLLTMTSKGLSNLRVI
                     DDEANIISFITELNYIKSQAIANQGYINVRFYENSDTIKVIENNKIRFLKLKVGKIIN
                     VAKVDIIAFDKKGNINKFGSITIDNNNSIYRIIFHIEKGRIRYEKL"
     gene            complement(932..1243)
                     /locus_tag="PPY92_002165"
     CDS             complement(932..1243)
                     /locus_tag="PPY92_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDJ2626148.1"
                     /translation="MFKFLKKTQAFTLIEMLLVLLIISLLLILIIPNIAKQTAHIQST
                     GCNAQVKMVNSQIEAYALKHNRNPSSIEDLIADGFIKEAQKTCKSGETITISNGEAVA
                     N"
     gene            complement(1257..2327)
                     /locus_tag="PPY92_002166"
     CDS             complement(1257..2327)
                     /locus_tag="PPY92_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDJ2626149.1"
                     /translation="MKLQWINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSF
                     QFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIGYSDTIVMQVYLAERFGNIIDVL
                     EETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
                     SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQ
                     LFKTYFVTNELVLFYKNGITLQSIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQIL
                     EKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIKQTQFLQPILFLILG
                     LFIVAIYLVIMLPMFQMMQSIK"
     gene            complement(2299..3273)
                     /locus_tag="PPY92_002167"
     CDS             complement(2299..3273)
                     /locus_tag="PPY92_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDJ2626150.1"
                     /translation="MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQ
                     IGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHFNKIYFLRISTLPLSLGQESCVI
                     RIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
                     VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVI
                     QASLSGHLVLTTLHATDCKGAILRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLV
                     CEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTMDKYI"
     gene            complement(3325..3948)
                     /locus_tag="PPY92_002168"
     CDS             complement(3325..3948)
                     /locus_tag="PPY92_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDJ2626151.1"
                     /translation="MRISSLTLGLVDTNTYFIENDKAVILIDPSGESEKIIKKLNQIN
                     KPLKAILLTHAHFDHIGAVDDIVDRFDVPVYMHEAEFDFLKDPVKNGADKFKQYGLPI
                     ITSKVTPEKLNEGSTEIEGFKFNVLHTPGHSPGSLTYVFDEFAVVGDTLFNNGIGRTD
                     LYKGDYETLVDSIQDKIFELEGDLPLFPGHGPYTTVDDEQLNPFLHG"
     gene            complement(3945..4274)
                     /locus_tag="PPY92_002169"
     CDS             complement(3945..4274)
                     /locus_tag="PPY92_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HDJ2626152.1"
                     /translation="MAIVDVVVIPVGTEGPSVSKYIADIQKKLQEYKAMGKIDFQLTP
                     MNTLIEGELSDVLEVVQVIHELPFDKGLSRVCTNIRIDDRRDKSRKMNDKLTSVQKHL
                     ENSGENL"
     gene            complement(4274..5260)
                     /locus_tag="PPY92_002170"
     CDS             complement(4274..5260)
                     /locus_tag="PPY92_002170"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HDJ2626153.1"
                     /translation="MTKIILAADVGGTTCKLGIFTPELEQLHKWSIHTDTSDSTGYTL
                     LKGIYDSFVEKVNENNYNFSNVLGVGIGVPGPVDFEKGTVNGAVNLYWPEKVNVREIF
                     EQFVDCPVYVDNDANIAALGEKHKGAGEGADDVVAITLGTGLGGGIISNGEIVHGHNG
                     SGAEIGHFRADFDQRFKCNCGRSGCIETVASATGVVNLINFYYPKLTFRSSILELIKE
                     NKVTAKAVFDAAKAGDQFCIFITEKVANYIGYLCSIISVTSNPKYIVLGGGMSTAGPI
                     LIENIKTEYHNLTFAPAQFETEIVQAKLGNDAGITGAAGLIKTYVLDKEGVK"
     gene            complement(5257..5460)
                     /locus_tag="PPY92_002171"
     CDS             complement(5257..5460)
                     /locus_tag="PPY92_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HDJ2626154.1"
                     /translation="MSRKINNFYDVQQLLKSYGFLIYFKNPEDMYEMIQQEISSLYQY
                     ELLSKEEYLKCTLIINQRRNEQK"
     gene            complement(5441..>5773)
                     /locus_tag="PPY92_002172"
     CDS             complement(5441..>5773)
                     /locus_tag="PPY92_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626155.1"
                     /translation="MTSGNYDNAQNIVKQTINKNYADDQTYYLSGMIMATINSKSEGM
                     TEWERGLRMFPKSGLLNFELAIANRSLNDDEKALKYVRKALNADPKNTDYINLEKELT
                     KSNESKNK"
BASE COUNT         1872 a         1007 c          740 g         2154 t
ORIGIN      
        1 gtaaaatgat acgctgttgc ctaatttcaa tatcgttttt atctacacct ttaaaatcct
       61 ctagaatatc tcttgagaaa aattcgaaat ctacagttgt taattcccta ctttcaatta
      121 gaaaagtttt gctaagtcta attaagtctg gaacaattag taaaattata cttataacca
      181 tcatcgctac taacatttca ataagcgaaa aagctttgac attaatactg tatacatgtc
      241 ttttgataag aagtggtatt ttttgaaata gcacaaattt gttggtcact ttgcttaata
      301 tcatatttcc ctatagttac ccccttctta agttcttctt tattaatttt agatacagtc
      361 gtcaaaatta ctttagaagc atcaattgat tgtagtttat ggtttatact cgcttgcatt
      421 tgattcatca ttggtatcaa tagtaatgta atcaatccaa ttagcaaaaa tccagccata
      481 ctatctatta agaatgatcc tttacactta tagcttttca taacgaattc ttcctttttc
      541 aatatggaat attattctat aaattgaatt gttattgtca attgttatgc taccaaattt
      601 attgatattc ccttttttat caaaggcaat aatatcaact tttgcaacat taattatttt
      661 gcctactttt aattttagaa atcgtatttt attattctct attactttaa tagtgtcact
      721 gttttcataa aatctaacat tgatatatcc ttgatttgct atagcttgcg acttaatata
      781 attcaattca gtaataaaag aaatgatatt tgcctcatca tctattactc taagattgct
      841 taatccttta gatgtcattg tcaaaagtag aaatatactg attaacatca ttaccacaag
      901 catctcaatc atagtaaatg ctgactgctt tctaatttgc aactgcttct ccattactaa
      961 ttgttattgt ctctcctgat ttacatgtct tttgtgcttc ttttataaaa ccatctgcaa
     1021 ttaagtcttc aatagacgat ggatttctat tatgtttcaa tgcatacgct tcaatttgac
     1081 tattaaccat ttttacctgt gcattacaac ctgttgattg tatgtgagca gtttgtttag
     1141 caatatttgg aatgattaaa attaataata aactgatgat taataacact aatagcatct
     1201 ctatcaatgt aaacgcttga gttttcttaa gaaatttaaa catatattaa cctccattat
     1261 tttatacttt gcatcatttg aaacattggt aacataatta ctaaataaat tgcgacaata
     1321 aataaaccta aaatcaaaaa taaaataggc tgtaaaaact gagtctgttt tatcgcttta
     1381 tcttctattt gttttactaa tatttgcgaa tataacttta gttctacttc tagcttccct
     1441 ctcttttcac cttgtagcac aaacttaatt aattgaggct taaagcattt tagtttttct
     1501 aaaatttgag gcaaaccata tcccatttct gaataagtta ataagtattt acctagaaac
     1561 tgtctaaatg gatcactact atggttaata taaacgtcta ctattgattg aagtgtaata
     1621 ccatttttat aaaacaacac taattcatta gttacaaaat aagttttaaa taattggaaa
     1681 tagcctgata ttagcggtag tttcatcaca aagtttatct tattgagcat atttaaattg
     1741 ttataaatta atttcataat aatagccaac atagatacta ttatgagcat tactacaatt
     1801 atagtaggta agctggtaat gaaaaaagac aatgtttttt gaaaagaaga tagttgaata
     1861 ttcatagaag tatataattg ttgaaactgt ggaattactg tgaggtttaa tataataatc
     1921 atagcaataa agatagaaac tagtattaag gggtattgca gtgtctttaa caatcgttgt
     1981 tctgactttc tattcacttt catataattt acggtttctt ctagaacgtc tataatattg
     2041 ccaaatcttt ctgccaaata tacttgcatg acgatagtat cgctataacc tatcagtgat
     2101 aatatttgat tgcatggtgc accatttgaa atttcactta gaatggtggt acctaattgc
     2161 ttatttttat atgtcatttg aagatttaaa aattgaaaac tttgatacag agtgaaacca
     2221 tatttcaaca aattacataa atttgaaagt aaatcgattt gttgggcctt acttaattgt
     2281 cgcttcttag aatgtagttt aaatgtattt atccattgta gtttcacaaa tgacacctgc
     2341 ttttgtcata tcatcaagtt tatcttctaa gttcttaaat gatgatggta atgaatgatt
     2401 atgggaaaag aaatatcgga gttgttgctg agatagaatt tcgcatacta attgtcgctg
     2461 ttgcttaata gtagttacaa gtcgttggtt tataattaag ttagttgcct gtatcaattc
     2521 ttgtacagaa atgcccattt ctaatagcct taaaatagca cctttacaat cagttgcatg
     2581 caatgtagtc agaacaaggt gaccacttaa actagcctgt ataacacact tggcaacatc
     2641 tttatctctg atttcaccta ttaaaataac atcaggatca catcttaaaa tagctttaaa
     2701 cgaatttaca tagttaatgc cagctttatc attcacatta atttggacga taccaggaat
     2761 ttgcatctct acaggatcct ctatagaaat tacatttaaa ttcaaggctt tattcgcgta
     2821 tgagaccatt tgatacatta atgtactctt tcctgaacca gttggcccac taaacaatag
     2881 taatccttgt ttcttattca tgaggtgttt aaaatcattg aatttataag ttgatttctg
     2941 ttgttgaaaa aattgaggta caattctgat aacacaactt tcttggccaa gtgacaatgg
     3001 taaagttgat attctcaaaa aatatatttt attgaaatgg taactatatc gaccgctctg
     3061 tgcgacttgc tgtgtagaaa catcaagccc agcttgaaac ttcatataaa ctaataactt
     3121 ttgataaatg ctattcccaa tttgttcata ctgctccaag ttatcattaa ttctaaattt
     3181 aatacttact tcatttttaa ctggaataaa atgtacatca ctcgctttca tttctatcgc
     3241 tttattaatt atttcttgaa atagaatctt caaaaaaaca cctcctacat ataatcacgt
     3301 aggaggtgtt tttattactt caatttaacc gtgtaaaaat ggatttaatt gttcatcatc
     3361 aaccgtcgta tatggaccat gtccagggaa taaaggtaaa tcgccttcta attcaaatat
     3421 tttatcttga atagaatcaa ctagcgtttc ataatcacct ttatataaat ctgtacgtcc
     3481 gattccatta ttaaataatg tatctccaac aactgcgaat tcatcgaaca catatgttaa
     3541 acttcctggt gaatgtccag gtgtgtgtaa cacattaaac ttaaatcctt ctatttctgt
     3601 gctaccttcg tttaactttt caggagttac cttacttgta ataattggta atccatattg
     3661 cttaaattta tctgctccat ttttaacggg atcttttaga aaatcaaact ctgcttcatg
     3721 catataaacc gggacatcga atcgatcaac tatatcatcg actgctccga tatgatcaaa
     3781 gtgtgcatgt gttaataaaa tagcttttaa cggtttattt atttggttta attttttaat
     3841 aattttttca ctttcacctg aagggtcaat cagaataaca gctttgtcat tttcgatgaa
     3901 atacgtatta gtatcaacta agcctaaagt taagcttgaa atcctcatag gttttcacca
     3961 ctattttcta aatgtttttg tactgatgtt agtttatcat tcatttttct agatttgtct
     4021 cgtcggtcat caatacggat atttgtacaa actctactta aacctttatc aaaaggtaat
     4081 tcatgtatca cttgcacaac ttctaataca tcgcttaatt caccttcaat tagagtattc
     4141 attggtgtta attgaaaatc aattttaccc attgctttat attcttgaag ttttttctga
     4201 atatctgcaa tatatttact aacactcgga ccttccgttc caactggaat aacaaccaca
     4261 tcaacaatag ccattatttt accccctctt tatctaatac ataggtcttg attaatcctg
     4321 ctgctcctgt aatacctgca tcattaccta atttcgcttg tacaatttca gtttcaaatt
     4381 gagcaggtgc aaatgttaaa ttatgatatt ctgttttaat attttcaatt aaaataggtc
     4441 ctgcagtaga cattcctcca cctagaacga tatatttcgg attacttgta acactaataa
     4501 tactacataa atatccaata tagtttgcaa ccttttcagt aatgaaaata cagaattggt
     4561 caccagcttt tgccgcatca aaaacagctt ttgctgtaac cttattttct ttaatcaatt
     4621 ctaatataga agatctaaac gtcaacttcg gatagtagaa gttaattaag ttaacaacgc
     4681 ctgtcgctga agcaactgtt tcaatacatc cagaacgacc acaattacat ttaaatcgtt
     4741 gatcgaagtc tgctctaaaa tgacctattt ctgcgccaga gccattatga ccatgtacga
     4801 tttcaccatt tgaaataatt cctccaccta gacctgtacc aagtgtgatg gcaacaacat
     4861 catcggcacc ttcaccagca cctttgtgtt tctcccctaa agcagctatg ttagcatcat
     4921 tatctacata cactggacaa tcaacgaatt gttcaaaaat ctcacgtaca ttaacttttt
     4981 ctggccaata taagtttact gctccattta ctgtaccttt ttcaaagtca acaggacctg
     5041 gtacaccaat acctacgcca agtacatttg aaaaattata attattttca tttacttttt
     5101 caacaaacga atcataaatt cctttcaaaa gtgtatatcc tgtactatca gatgtatcag
     5161 tgtgaataga ccatttatgt aattgttcta attcaggtgt gaaaatacct aatttacaag
     5221 tcgtcccgcc tacatcagct gctaaaataa tttttgtcat ttctgttcat tccttctctg
     5281 attaattatc aacgtacatt tcaaatattc ttccttagat aacagttcat attgatacaa
     5341 tgatgaaatc tcctgttgaa tcatttcgta catatcttct ggatttttaa aatatattag
     5401 aaatccgtaa cttttcaata actgttgtac atcataaaag ttatttattt ttcgactcat
     5461 ttgatttagt caactctttt tctaagttaa tataatctgt atttttaggg tctgcattta
     5521 atgctttacg cacatatttt aatgcttttt catcatcatt taatgaacga tttgctatcg
     5581 ctaactcaaa atttaataaa cctgatttag gaaacattct aagtcctcgc tcccattctg
     5641 tcataccttc ggacttagaa ttgatagtag ccataatcat accacttaaa taatatgttt
     5701 gatcatcggc ataattttta tttattgtct gcttcacaat attttgagca ttatcataat
     5761 taccactagt cat
//
LOCUS       DANBWH010000170         5740 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.176, whole genome shotgun sequence.
ACCESSION   DANBWH010000170 DANBWH010000000
VERSION     DANBWH010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.176"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..833
                     /locus_tag="PPY92_002173"
     CDS             <1..833
                     /locus_tag="PPY92_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDJ2626156.1"
                     /translation="SPFNDAKLNAEQKEILNEKYGLNDPVATQYLHYLKNVVTGDFGN
                     SFQYHNQPVWDLIKPRLLPSFEMGLTAMFIGVILGLILGVAAATKQNSWVDYTTTVIS
                     VIAVSVPSFVLAVLLQYVFAVKLRWFPVAGWEGFSTAVLPSLALSAAVLATVARYIRA
                     EMIEVLSSDYILLARAKGNSTMRVLFGHALRNALIPIITIIVPMLASILTGTLTIENI
                     FGVPGLGDQFVRSITTNDFSVIMAITLLFSTLFIVSIFIVDILYGVIDPRIRVQGGKK
                     "
     gene            833..1903
                     /locus_tag="PPY92_002174"
     CDS             833..1903
                     /locus_tag="PPY92_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDJ2626157.1"
                     /translation="MAENKNNLSINDDHSNAAMTHTSDAIASSDFIIRELDLNQEPEM
                     QRESKNFWQDAWAQLKRNKLAVVGMIGLIIIVIFAFIGPVINKHDYAEQNVEHRNLPA
                     KIPVLDKVPFLPFDGKDADGKDAYKAANAKENYWFGTDQLGRDLWTRTWKGAQISLFI
                     GVVAAMLDIFIGVVYGAISGFFGGRVDTIMQRILEVIASIPNLIVVILFVLIFEPSIW
                     TIILAMSITGWLGMSRVVRGEFLKLKNQEFVMASKTLGASKFQLIFKHILPNTLGAIV
                     VTSMFTVPSAIFFEAFLSFIGIGVPAPQTSLGSLVNDGRAMLLIYPHELFIPAMILSL
                     LILFFYLFSDGLRDAFDPKMRK"
     gene            1919..3001
                     /locus_tag="PPY92_002175"
     CDS             1919..3001
                     /locus_tag="PPY92_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDJ2626158.1"
                     /translation="MTERILEVNDLHVSFDITAGEVQAVRGVDFYLNKGETLAIVGES
                     GSGKSVTTKAITKLFQGDTGRIKKGEILFLGEDLAKKPENELIKLRGKDISMIFQDPM
                     TSLNPTMQIGKQVMEPLIKHKNYSKAQAKKRALEILNLVGLPNAEKRFKAYPHQFSGG
                     QRQRIVIATALACEPKVLIADEPTTALDVTMQAQILDLMKELQQKIDTAIIFITHDLG
                     VVANIADRVAVMYGGQMVETGDVNEIFYDPKHPYTWGLLSSMPDLSTTNDTPLLAIPG
                     APPDLLHPPKGDAFARRSQYALDIDFKVEPPWFKVSPTHFVKSWLLDARAPRVELPEL
                     VKQRMKPMPNNYEKPLKVERVSFNEK"
     gene            2991..3932
                     /locus_tag="PPY92_002176"
     CDS             2991..3932
                     /locus_tag="PPY92_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDJ2626159.1"
                     /translation="MKNDEVLLSIKNLKQYFNAGKKNEVRAIENISFDIYKGETLGLV
                     GESGCGKSTTGKSIIKLNDITSGEILYEGIDIQKIRKRKDLLKFNKKIQMIFQDPYAS
                     LNPRLKVMDIVAEGIDIHHLATDKRDRKKRVYDLLETVGLSKEHANRYPHEFSGGQRQ
                     RIGIARALAVEPEFIIADEPISALDVSIQAQVVNLLLKLQRERGITFLFIAHDLSMVK
                     YISDRIAVMHFGKIVEIGPAEEIYQNPLHDYTKSLLSAIPQPDPESERSRKRFSYIDD
                     EANNHLRQLHEIRPNHFVFSTEEEAAQLRENKLVTQN"
     gene            3951..5606
                     /locus_tag="PPY92_002177"
     CDS             3951..5606
                     /locus_tag="PPY92_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDJ2626160.1"
                     /translation="MTRKFRTLILILIATIALSGCANDDGIYSDKGQVFRKILSSDLT
                     SLDTSLITDEISSEVTAQTFEGLYTLGKGDKPVLGVAKAFPEKSKDGKTLKVKLRSDA
                     KWSNGDKVTAQDFVYAWRKTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALN
                     DETLQIELEKPVPYINQLLALNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWK
                     QEDKTLLSKNQYYWDKKNVKLDKVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYK
                     DNKGLNFVLTSGTFFVKMNEKQYPDFKNKNLRLAIAQAIDKKGYVDSVKNNGSIPSDT
                     LTAKGIAKAPNGKDYASTMNSPLKYNPKEARAHWDKAKKELGKNEVTFSMNTEDTPDA
                     KISAEYIKSQVEKNLPGVTLKIKQLPFKQRVSLELSNNFEASLSGWSADYPDPMAYLE
                     TMTTGSAQNNTDWGNKEYDQLLKVARTKLALQPNERYENLKKAEEMFLGDAPVAPIYQ
                     KGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDKSIDKETGKKKK"
BASE COUNT         2063 a          840 c         1103 g         1734 t
ORIGIN      
        1 gttcgccatt taacgatgct aaattaaatg ctgaacaaaa agaaatttta aatgaaaaat
       61 atggattaaa tgatcctgta gctacgcagt atttacatta tttaaaaaat gttgttacag
      121 gcgattttgg taattcattc cagtatcata atcaacctgt gtgggatttg attaaaccga
      181 gactactacc ttcttttgaa atgggtctta cagcaatgtt catcggtgtg atactgggac
      241 ttattttagg tgttgcagca gctactaaac aaaattcttg ggttgactat acaactacag
      301 ttatttcagt tattgctgta tctgtaccat cttttgtact tgctgtactt ttacaatatg
      361 tatttgcagt taaattaaga tggttcccag tagctggatg ggaaggtttt tcgaccgcgg
      421 tattaccgtc acttgcatta tctgcagctg ttttagcaac tgtcgccaga tacataagag
      481 cagagatgat agaggtatta agttcagact atattttatt agcgagagct aaaggtaatt
      541 cgacaatgcg tgtacttttt ggacatgcac ttagaaatgc tttaattcca attattacaa
      601 ttatcgttcc catgttagca agtattttaa caggcacttt aacaattgaa aatatttttg
      661 gggttcctgg attaggggat caattcgtac gttcaattac aacaaatgat ttctcagtaa
      721 tcatggcaat cacactatta tttagcacac tgtttatcgt ttctattttt attgtagata
      781 ttttgtacgg tgtgatagat ccacgaattc gtgttcaagg aggtaaaaaa taatggctga
      841 aaataaaaac aatttgtcga ttaacgacga tcattctaat gcagctatga cgcatacctc
      901 tgacgctatc gcatcatctg attttattat tagagaatta gacttgaatc aggaacctga
      961 aatgcaacga gaaagcaaaa acttttggca agatgcttgg gctcagttaa aacgaaataa
     1021 gttagctgtt gtcggtatga taggtttaat tatcattgta atatttgctt ttatcggtcc
     1081 agttataaat aaacatgatt atgctgaaca aaatgtagaa catagaaatc ttccggcaaa
     1141 aatacctgta ttagacaaag ttccattttt accttttgat ggtaaagatg cagatggcaa
     1201 ggatgcttat aaagcagcaa atgctaaaga aaattattgg tttggtactg atcagttggg
     1261 tcgagattta tggacaagaa catggaaagg tgctcaaatt tcattgttta tcggtgttgt
     1321 tgcagcgatg ttagatattt ttattggcgt tgtatatggt gcgatttctg gattcttcgg
     1381 tggacgtgtc gatacgatta tgcaacgtat acttgaagtc atagcatcta ttccgaattt
     1441 aattgtcgta attttatttg tattaatttt tgaaccatcc atttggacaa ttatattggc
     1501 tatgtctatc acaggctggt taggcatgag cagagttgta cgtggagaat ttttaaaatt
     1561 gaaaaatcaa gagtttgtca tggcttcgaa aacattgggg gcttcaaaat tccaattgat
     1621 atttaagcat attttaccta atacgttagg tgctatcgtg gttacatcaa tgtttacagt
     1681 acctagtgct attttcttcg aagcattttt aagtttcatt ggtataggtg tacctgcacc
     1741 tcaaacatcg ttagggtcat tagtaaatga tgggcgcgca atgttattaa tttatccaca
     1801 tgaattattt ataccagcaa tgattttaag tttattaatt ctattctttt acttatttag
     1861 tgatggatta cgtgatgcat ttgatccgaa aatgcgtaaa taaaaggggg catagcatat
     1921 gactgaaaga atattagaag taaatgattt gcatgtttcc tttgatatta cagcagggga
     1981 agtgcaggca gtgagaggtg tagattttta tttaaacaag ggggaaacat tggcaattgt
     2041 tggtgaatca ggttcaggta aatctgtaac aacaaaagca attacaaaat tattccaagg
     2101 ggacacagga agaattaaaa agggagaaat tttattttta ggggaagatt tagcaaaaaa
     2161 acctgaaaat gagttaatta aattacgtgg caaagatatt tcaatgatct ttcaagatcc
     2221 aatgacatct ttaaacccaa cgatgcaaat tggtaaacaa gtcatggaac cattaattaa
     2281 gcacaaaaat tatagtaaag cacaagctaa aaagcgcgca ttggaaatac taaatcttgt
     2341 aggtttacca aatgcagaaa aaagatttaa agcatatcct catcaatttt caggtggaca
     2401 aaggcaaaga attgttattg caaccgcatt agcttgtgaa cctaaagtgc tcattgctga
     2461 tgaaccaacg actgcattag acgtaacgat gcaggcacaa attttagatt taatgaaaga
     2521 actacaacaa aaaatcgata cagcaattat ttttataacg catgatttag gggttgttgc
     2581 gaatattgct gatagagtgg cagttatgta tggtggtcaa atggttgaaa caggagatgt
     2641 taacgaaata ttttatgatc caaagcatcc atatacatgg ggattattat cgtcaatgcc
     2701 tgatttatca acaacaaatg acacaccatt actagcgatt cctggagcgc cacctgattt
     2761 attacaccca cctaaaggtg atgcatttgc gagacgtagt caatatgcat tagatattga
     2821 ttttaaagta gaaccaccgt ggtttaaagt ttcaccgaca cattttgtga aatcttggtt
     2881 attagacgca cgtgcaccaa gagttgaact acccgagctg gtaaaacaac gtatgaaacc
     2941 gatgcctaat aattatgaaa aaccactcaa ggtagaaagg gtgtcgttca atgaaaaatg
     3001 atgaagtgct attatctatt aaaaatttaa agcaatattt taacgcagga aagaaaaacg
     3061 aagtgagagc gattgaaaat atttcgtttg atatatacaa aggggaaaca ttaggtttag
     3121 taggagaatc ggggtgtggt aaatctacaa ctggtaaatc aattattaaa cttaatgata
     3181 ttacaagtgg agaaattttg tatgagggta ttgatataca aaagattcgt aaacgtaaag
     3241 atttgcttaa atttaataaa aagatacaga tgatttttca agacccatat gcgtctttaa
     3301 atcctaggtt aaaagtaatg gatatagtag ctgaaggtat tgatatccat catttagcaa
     3361 ctgataagcg tgaccgaaaa aaacgtgtct atgatttact tgaaactgtt ggattaagta
     3421 aagaacatgc caatcgctat cctcatgaat tttcaggtgg acaacgccaa cgtattggaa
     3481 ttgcccgtgc attagccgtt gaaccagaat tcattatcgc ggacgaacca atatcggcat
     3541 tggatgtttc aatccaagct caagtagtta atttattatt aaaattacaa cgtgaaagag
     3601 ggattacgtt cctatttata gctcatgatc tatcaatggt gaagtatatt tcagatcgta
     3661 ttgcagtcat gcattttggg aaaatagttg aaattggacc ggcagaagaa atttatcaaa
     3721 atccattaca cgattatact aagtctttat tatcagccat tccacaacct gatcctgaat
     3781 cagaacgcag tcgcaaacga tttagttata ttgatgatga agcaaataat catttaagac
     3841 aattacatga aattagaccg aatcactttg tctttagtac tgaagaagaa gcggcacaac
     3901 tacgagaaaa taaattggtg acacaaaatt aaggggaagg gggaaatgca atgacgagaa
     3961 aatttagaac acttatttta attttgattg ctacaattgc attaagtggt tgtgctaatg
     4021 acgatggtat ttattcagat aaaggtcaag tattcagaaa aattttgtca tcagacttaa
     4081 catcccttga tacatcatta ataacggatg aaatatcttc tgaagtgact gcgcaaacat
     4141 tcgaaggttt atacacatta ggaaaaggtg acaaaccggt gttaggtgtt gcgaaagctt
     4201 ttcctgaaaa gagtaaagat ggtaaaactt taaaggttaa attaagaagc gatgctaaat
     4261 ggagcaatgg tgacaaagtg actgcacaag actttgttta tgcttggaga aaaacagttg
     4321 accctaaaac aggttctgaa tttgcataca ttatggggga cattaaaaat gcgagtgata
     4381 ttagtactgg taagaaacct gtagagcaat taggtatcaa agcattaaat gatgaaacat
     4441 tacaaattga attagaaaag ccggttccat atattaatca attattagca cttaatacct
     4501 ttgcacctca aaatgaaaaa gttgccaaaa aatatggtaa aaattacggt acggcagctg
     4561 atagagcggt atacaatggt ccatttaaag ttgatgattg gaaacaagaa gataaaacct
     4621 tactatctaa aaatcagtat tattgggata aaaagaatgt aaaattagat aaagtgaatt
     4681 ataaagttat taaagactta caagccggtg catcattgta tgatactgaa tcagtagatg
     4741 acgcagttat tactgcagat caagtaaata aatataaaga caacaaagga ttaaactttg
     4801 tgttaacgtc tgggacattt tttgtaaaaa tgaatgaaaa acaatatcct gattttaaaa
     4861 acaaaaattt aagattggct atcgcacaag caatagataa aaaaggatac gttgattcag
     4921 tgaaaaacaa tggctcaatt ccttccgata cactaacagc caaaggaatt gcgaaagcgc
     4981 ctaatggcaa agattatgcg agtaccatga attcgccttt aaaatataat cctaaagaag
     5041 caagagcaca ctgggacaaa gctaaaaaag agttaggtaa aaatgaagtg acattttcaa
     5101 tgaacacaga agatacacca gatgcaaaaa tatctgctga atatatcaaa tcgcaagttg
     5161 agaaaaattt accaggagtt actttgaaaa ttaagcaatt accgtttaaa caaagagtat
     5221 cactagaact gagtaacaat tttgaagcat cacttagtgg ttggtctgca gattaccctg
     5281 atcctatggc ttatttagaa acaatgacca caggtagcgc acaaaataat acagactggg
     5341 gtaataaaga atatgatcaa ttacttaaag tagcaagaac caaattggca cttcaaccga
     5401 acgaacgata tgaaaacttg aaaaaagcag aagaaatgtt cctaggagat gcaccggtag
     5461 caccaattta tcaaaaaggt gttgcacatt taacaaatcc tcaagtaaaa ggattaattt
     5521 accataaatt tggtccaaat aactcactta aacatgtata tattgataaa tcgatagata
     5581 aagaaacagg taagaagaaa aaataatatg ctttgtaaat taggctggag acatatctcc
     5641 agtctttttg tgttggataa aaactttggg aataaaaatt taaaataagt cgttttttaa
     5701 attactgaaa ttgattaaat gcataaataa ctgaatattc
//
LOCUS       DANBWH010000171         5445 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.177, whole genome shotgun sequence.
ACCESSION   DANBWH010000171 DANBWH010000000
VERSION     DANBWH010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.177"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(20..1582)
                     /locus_tag="PPY92_002178"
     CDS             complement(20..1582)
                     /locus_tag="PPY92_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDJ2626161.1"
                     /translation="MKNKSVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITTFIAL
                     HFSWYYLLLVLVILFVCVYILFSRYANITLGEEGEDPEFSLPSWFAMLFSAGMGIGLV
                     FWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFH
                     KGYPGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFL
                     FNVPSNFTMQVILIVIATILFTWSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTL
                     YILNTFTNGLGNYIANFFSMSLRIPSGGQKFQWLQNWTIFYWAWWISWAPFVGIFIAR
                     VSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIADIAKAATETATFATLQ
                     HYPLGFVLSLITLLVIMIFFVTSADSATYVLGMLSSRGDINPKSFVKVSWGIIMALFA
                     IIMIYTGGTQAIQNLLIIAALPFSVVIIAMIWSLLKSLSEEKPRNSNKVLIKHRDPDI
                     LEYRSSKHIDEN"
     gene            complement(1890..2897)
                     /locus_tag="PPY92_002179"
     CDS             complement(1890..2897)
                     /locus_tag="PPY92_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDJ2626162.1"
                     /translation="MKMIGFEKPFKLEEGNLFKVYEQRKPTPENDDILVKVNSISVNP
                     VDTKQRQMKVTQAPRVLGFDAIGTVEAIGPDVTLFSPGDVVFYAGSPNRQGSNATYQL
                     VSEAIVAKAPHNISANEAVSLPLTGITAYETFFDTFKISHNPSENVGKSVLIINGAGG
                     VGSIATQIAKRYGLTVITTASRQETTEWCEKMGADIVLNHKEDLVRQFKEKEIPLVDY
                     IFCTYNTDLYYNTMIELIKPLGHITTIVAFNEDQDLNALKLKSITFTHEFMFARPIHR
                     TPDMIKQHEYLEDITKNIELGHYQPTTTQVFEGLSPENLYQAHQLLEKQSMIGKLVIN
                     I"
     gene            complement(3132..4133)
                     /locus_tag="PPY92_002180"
     CDS             complement(3132..4133)
                     /locus_tag="PPY92_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HDJ2626163.1"
                     /translation="MQNKQILFNKIPEGMPQEDTFKIEEIDTPKLESDGVLVQTLYIS
                     VDPYMRGRMTKADSYVQPFEIGKPIVSHVVAKVIDSTLADYKKGDVVVGMLPWRIINH
                     VQADQITKVPTTDVPLDLYLSVLGMPGQTAYHGLLDIGQPKAGDTVVVSAASGAVGSV
                     VGQIAKIKGCRVVGIAGGDKKVNYLTETLGFDAGIDYKKEDFAEALAQAVPDGIDVYF
                     ENVGGTVGDEVFKHLNRFARVPVCGAISSYNHPEADIGPRIQGTLIKKQVMMRGFLVS
                     EFANAFKEASEQLATWVQEGKIQSQVTIEDGFENAPHAFKNLFTGDNFGKQVIKVTE"
     gene            complement(4411..5280)
                     /locus_tag="PPY92_002181"
     CDS             complement(4411..5280)
                     /locus_tag="PPY92_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDJ2626164.1"
                     /translation="MKKIFVIITTLLAVAIIIGSIITVVFSQRQAQTFKIQQQQFVKK
                     PIPTLFLHGFGGSANSEKFMVKQAEKRGVTKDIITAYVSKDGAVTFKGKLRKDAVNPI
                     VKIELENNRQGYLDKNAAWFKNVLTKLQSEYNFDKFNFVGHSMGNLTFAQYMMTYGND
                     KSLPQLNKQVNIAGTFNGVLNMNEDVNEITVDKDGKPSRMNQPYQQLRVLKDIYKGKG
                     IEVLNIYGDLKDGTHSDGRVSNSSSKSLKYLLGNSPKSYRESKYEGEPAQHSQLHENE
                     NVANELIDFLWKK"
BASE COUNT         1732 a         1057 c          790 g         1866 t
ORIGIN      
        1 aataaaacct cattattaat taattttcgt caatatgttt tgaagaacga tattctaaaa
       61 tatctgggtc acgatgttta attaaaacct tattactatt tctaggtttt tcttcactta
      121 aagattttaa aagcgaccat atcatcgcta taatgaccac ggaaaatggt aacgcagcaa
      181 tgattaataa attttgaatc gcttgagtac cacctgtgta gatcatgata attgcaaata
      241 atgccataat gataccccaa ctcactttga caaatgactt cggattaata tcacctcttg
      301 aactcaacat acctaaaaca taagttgccg aatccgctga tgtaacaaag aaaatcataa
      361 taacaagtaa agtaattaag cttaatacaa aacctagtgg gtaatgttgt agcgttgcaa
      421 aagttgctgt ttctgtcgct gctttagcaa tatcggcaat atgattatct tgtaagtaaa
      481 ttgctgacgc gccgaatacc gcaaagaata taaagcaaac taacgccggg acaaaaagta
      541 cacctagaat aaattcttta atcgtacgtc cttttgacac acgtgcaata aatataccta
      601 caaatggtgc ccaagatatc caccatgccc agtaaaagat tgtccaattt tgtaaccatt
      661 ggaatttttg accgcctgac ggaatacgta aactcatact aaagaaattt gcaatataat
      721 tacctagacc atttgtaaat gtatttaaaa tgtaaagcgt tggcccaaca ataaaaagac
      781 caataagtac tacaaaagca agtaacatgt tgatattact caacgttttg atacctttat
      841 cgatacctga ccatgctgac caagtaaata atatggttgc aatgacaatc aagattactt
      901 gcatcgtgaa gttactcggt acattaaata aaaaatgtaa accttcgttt atttgcaatg
      961 caccgaaacc taatgttgca gcaacgcctg ttactgtagc tatgactgcc aatacatcaa
     1021 tggcaccacc taaaggtcca cgcattgcct tttcacctaa taaaggcgtc aatgtagcgc
     1081 tgactaagcc aggatatcct ttatgaaagc taaaatatgc aaacacaagc gcgacaatac
     1141 catagactgc ccatgcatga atcccccaat ggaaaaatga aaactgcatt gcatcattaa
     1201 ttgcagattg cgtgccagct ttatgaatag gcgttaattt gaaggcatga ctgattggtt
     1261 ctgccgttgt ccagaacaca agtcctattc ccataccagc actaaataac atagcaaacc
     1321 aagacggcaa tgagaattca ggatcttcgc cttcttcacc taatgtaatg tttgcgtatc
     1381 tcgaaaataa aatatacaca cagacaaata aaataactaa aacgagcaat aaataatacc
     1441 aagaaaaatg tagcgcaata aatgtagtaa tgttttgcgt gagtttttct aattgtttcg
     1501 gaaatattgc tccaaaagca acaaatatcg tacatatcac taaagatacc caaaacacta
     1561 gacttactga tttatttttc ataaatacaa accctttctg tgtaatggta agttcatacc
     1621 cataactgca acattttaat catttgtaat tttatataga cacaattaat aatgcctcat
     1681 cttttaaaaa tgatatataa aacacactca aattatttat cattgagcaa caaagtattt
     1741 tattgtattt aagtaatgcc tttctagtgc attattgatt tgatacctgt aaagttgcca
     1801 tatttccgtt tagaatcaat agtcgctaga cacaaaaaat aagtcgccct atacagtatt
     1861 ttctgcataa ggcgacttta cttactaatc tatatattaa ttactaattt cccaatcatt
     1921 gattgttttt ccaacaattg atgtgcttga tataagtttt caggtgataa accttcaaaa
     1981 acttgtgtcg ttgttggttg gtaatgccct aattctatat ttttcgtaat atcttctaaa
     2041 tactcatgtt gtttaatcat atcaggcgtt cgatgaattg gacgcgcaaa cataaattca
     2101 tgtgtaaatg ttatactttt taattttaat gcatttaaat cttgatcttc attaaaagct
     2161 acgatagtcg taatatgccc taatggtttt atcagttcaa tcatagtatt gtaatacaag
     2221 tctgtattat aagtgcaaaa tatataatct actaatggaa tttctttttc tttaaattga
     2281 cgcactaaat cctctttatg attcaatacg atatctgcgc ccatcttttc acaccactct
     2341 gttgtttctt gtcgtgatgc tgttgtaatg acagttaaac cataccgttt agcaatttga
     2401 gtggctatac tgcctacacc accggcacca ttaatgatta acacggattt acctacattt
     2461 tcagaaggat tatgtgaaat tttaaatgta tcaaaaaacg tttcatatgc cgtaatacca
     2521 gttagcggta gactaaccgc ttcattagca cttatgttgt gtggtgcctt tgcaactata
     2581 gcttctgaca ccaattgata tgtcgcattt gatccttgtc tatttggcga tccagcataa
     2641 aatacaacgt cacccggact aaataatgta acgtctggtc cgatagcttc aacagtacca
     2701 atagcatcaa acccaagtac acgaggtgct tgagtgactt tcatttgacg ttgttttgta
     2761 tctacaggat ttacactaat actatttact ttgactaaga tatcatcgtt ttctggcgtt
     2821 ggctttcttt gttcgtatac tttaaataaa tttccttctt ccaatttaaa gggcttttca
     2881 aatcctatca ttttcatatc gtttcacctc atttatgaac ttatttctta ttatacaaaa
     2941 tagaagccat gtgtgcttat accgcagcat catgactcct ttttcatttg aatatataaa
     3001 taattacaga cgactttcgt attacatttt agacttattt ctaccatgtt actgaacaaa
     3061 tttgctttag ataagaaagt tattaaattt tgatcaatta acaaagttag tttgttaaaa
     3121 cgtatacttt attattccgt tactttaata acttgtttac caaagttatc gccagtaaat
     3181 aaatttttaa atgcatgtgg cgcattttca aaaccatctt caatggttac ttgtgactga
     3241 attttacctt cttgaaccca tgttgcgagc tgttcactag cttctttaaa agcattagcg
     3301 aattcactta ctaagaagcc tctcatcatt acttgcttct taataagcgt accttgaata
     3361 cgtggtccga tatctgcttc aggatgatta tatgacgaaa ttgcgccgca tactggtaca
     3421 cgtgcaaaac gatttaaatg cttaaatact tcatcgccaa ctgtaccacc aacattttca
     3481 aaataaacat caataccatc tggtactgct tgtgctaacg cttctgcaaa atcctctttc
     3541 ttataatcaa taccagcgtc aaagcccagt gtctctgtta aataatttac ttttttgtcg
     3601 ccacccgcaa tacctactac acggcaacct ttaatcttag caatttgacc tacaactgaa
     3661 cctacagcac cagatgcagc tgaaaccaca acagtatcac cggctttagg ttgtccaata
     3721 tcaagcagac catgatatgc tgtttgtcct ggcattccta aaacacttaa atataaatca
     3781 agtggtacat ctgtcgttgg aactttagta atttgatccg cttggacatg attaatgatt
     3841 cgccaaggca acatacctac aacgacatct ccttttttat aatctgcgag tgtcgaatca
     3901 attacttttg caacgacatg gctaacaatc ggtttaccaa tttcaaaagg ctgtacatac
     3961 gaatctgcct tagtcatacg tcctctcata tatggatcca ctgaaatata cagcgtttgt
     4021 acaagtacac catcgctctc aagtttaggc gtgtcaatct cttcaatttt gaatgtatcc
     4081 tcttgaggca tgccttctgg tattttgtta aaaagaattt gtttattttg catcattaat
     4141 cacctttctt tatttgaaac ttttacttat ttgttactta agcgttaagt tttaattgtg
     4201 tcttcgtgat gtctgtatgc aaatacattc ttagttgtta tattttgact taagcactga
     4261 ttcatttaac ttcaagtaca ttatatttgc tgtaaacata aattaaaaat gttacgactt
     4321 agacatttta tggaaactct caaacaatag ataatttttg aaaagctcta atattacaag
     4381 cttttttgcc ccagaaaact agcagttgct ttattttttc cataagaagt cgattaactc
     4441 attagcaaca ttttcattct catgaagctg actatgttgt gcaggctcac cttcatattt
     4501 agattctcga taacttttcg gactatttcc cagtaaatat tttaatgatt tcgaagaact
     4561 attagacact ctgccgtctg aatgtgtacc atctttcaaa tctccataaa tatttagcac
     4621 ttcgatacct ttacctttat aaatatcttt tagcacgcgt aattgctgat aaggttgatt
     4681 cattcgactt ggtttaccat ctttatcaac tgtaatttca ttgacatctt cattcatatt
     4741 taaaacacca ttaaatgtcc ctgcaatatt cacttgtttg tttaactgtg gcagtgactt
     4801 gtcgttacca tatgtcatca tatattgtgc aaatgttaag ttccccattg agtgaccgac
     4861 aaagttgaat ttatcgaaat tgtattcaga ttgtaactta gtcagtacat ttttaaacca
     4921 cgcagcattc ttatccaaat agccttgtct gttattttca agttcaattt tcacaatagg
     4981 attcactgca tcttttctta gtttcccttt aaatgtcact gcaccatcct ttgaaacgta
     5041 agcagtgatg atatctttag ttacccctct tttttctgct tgcttcacca taaacttttc
     5101 agaattggca ctaccaccaa atccatgtaa gaacaatgtt ggaattggct ttttaacaaa
     5161 ttgctgttgt tgtattttaa atgtttgtgc ctgtcgttga ctaaacacca ccgtaataat
     5221 agagcctata ataatagcga ccgctaacaa tgtcgtaata attacaaaaa ttttcttcac
     5281 acttttaact cccattcatg tcttttatat aagtataaag gatgtgatta aaaatgtcct
     5341 ttagttgatt ttgaatacat cattaacttt taagatgact ttggaaagtt gtccgttaac
     5401 gtttgttaat tgattgcttc tttagctttc aatggtgtgt caccc
//
LOCUS       DANBWH010000172         5436 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.178, whole genome shotgun sequence.
ACCESSION   DANBWH010000172 DANBWH010000000
VERSION     DANBWH010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.178"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            231..1406
                     /gene="rpsA"
                     /locus_tag="PPY92_002182"
     CDS             231..1406
                     /gene="rpsA"
                     /locus_tag="PPY92_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDJ2626165.1"
                     /translation="MTEEFNESMINDIKEGDKVTGEVQQVEDKQVVVHINGGKFNGII
                     PISQLSTHHIDSPSEVVKEGDEVEAYVTKVEFDEENETGAYILSRRQLETEKSYSYLQ
                     EKLDNNEIIEAKVTEVVKGGLVVDVGQRGFVPASLISTDFIEDFSVFDGQTIRIKVEE
                     LDPENNRVILSRKAVEQEENDAKKDQLLQSLNEGDVIHGKVARLTQFGAFIDIGGVDG
                     LVHVSELSHEHVQTPEEVVSIGQDVKVKIKSIDRDTERISLSIKDTLPTPFENIKGQF
                     HENDVIEGVVVRLANFGAFVEIAPGVQGLVHISEIAHKHIGTPGEVLEPGQQVNVKIL
                     GIDEENERVSLSIKATLPNEDVVESDPSTTKAYLENEEEDNPTIGDMIGDKLKNLKL"
     gene            1628..2938
                     /gene="der"
                     /locus_tag="PPY92_002183"
     CDS             1628..2938
                     /gene="der"
                     /locus_tag="PPY92_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019167598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDJ2626166.1"
                     /translation="MTKPIVAIVGRPNVGKSTIFNRIVGERVSIVEDTPGVTRDRIYS
                     SGEWLTHDFNIIDTGGIEIGDAPFQTQIRAQAEIAIDEADVIIFMVNVREGLTQSDEM
                     VAQILYKSKKPVVLAVNKVDNMEMRTDVYDFYSLGFGEPYPISGSHGLGLGDLLDAVV
                     SHFGEEEEDPYDEDTIRLSIIGRPNVGKSSLVNAILGEDRVIVSNVAGTTRDAIDTEY
                     SYDGQDYVLIDTAGMRKKGKVYESTEKYSVLRALKAIERSNVVLVVIDAEQGIIEQDK
                     RVAGYAHEQGKAVVIVVNKWDTVEKDSKTMKKFEDEVRKEFQFLDYAQIAFVSAKERT
                     RLRTLFPYINEASENHKKRVQSSTLNEVVTDAISMNPTPTDKGRRLNVFYATQVAIEP
                     PTFVVFVNDVELMHFSYKRYLENQIRAAFGFEGTPIHIIARKRN"
     gene            2955..3953
                     /locus_tag="PPY92_002184"
     CDS             2955..3953
                     /locus_tag="PPY92_002184"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDJ2626167.1"
                     /translation="MTKITVFGMGSFGTALANVLAENGHDVLMWGKNQDAVDELNTCH
                     TNKKYLKYAKLDVNIIATSDMTKAIQFADIYLMALPTKAMREVATQINDKLTSKKTFI
                     HVAKGIENGTFKRVSEMIEDSISPEYNAGIGVLSGPSHAEEVVVKQPTTVAASSKDKS
                     VSKLTQDLFMNDYLRVYTNDDLIGVELGGALKNIIAVASGIVAGIGYGDNAKAALMTR
                     GLAEISRLGEKLGADPMTFLGLGGIGDLIVTCISTHSRNFTLGYKLGQGESMDQALSE
                     MNMVVEGIYTTKSVYHLAKEKNVDMPITNALYRVLFENISVKECVKDLMERDKKSE"
     gene            4124..4396
                     /locus_tag="PPY92_002185"
     CDS             4124..4396
                     /locus_tag="PPY92_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDJ2626168.1"
                     /translation="MNKTDLINAVAEQADLTKKEAGSAVDAVFESIQNSLAKGEKVQL
                     IGFGNFEVRERAARKGRNPQTGKEIDIPASKVPAFKAGKALKDAVK"
     gene            4827..5399
                     /locus_tag="PPY92_002186"
     CDS             4827..5399
                     /locus_tag="PPY92_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenyl diphosphate synthase component 1"
                     /protein_id="HDJ2626169.1"
                     /translation="METTVSKLERQIEERLKGVSEYESININHRLGKLLDSYDIPDVA
                     KVACLTIDTSMRHLDDITYNHLSKHSILIGDLISAHFYTLLAEINDLSFQNEISKAIV
                     EINELKSSLHHQALNDYEISQAIVKIETLFPYITLSHFGINIDESEIYNYLFEDMSDY
                     YPSYFKKYNQSEVKHYLHDIQKSYLKSRGN"
BASE COUNT         1995 a          711 c         1033 g         1697 t
ORIGIN      
        1 taagctatgc taatttttat acaaaaaata tgtataaaca tagcggactt tattacttgt
       61 ttgaatatat ggttgtaata aatatatgtc caactaattt acagaatgag attaagtgac
      121 atatttatgg atacaactaa tttactaaat gtaataaagg tagaatttct tgacaattct
      181 gtcagtttat aagatgttat aaatatgtag tgtataagga ggcaaacaag atgactgaag
      241 aattcaatga atcaatgatt aacgatatta aagaaggtga caaagtcact ggcgaggtac
      301 aacaagttga agacaagcaa gttgttgttc atatcaacgg tggtaaattt aatgggatta
      361 ttcctattag tcaactatct acgcatcata ttgatagccc aagtgaagtt gtaaaagagg
      421 gcgacgaagt tgaagcatat gtcactaaag ttgagtttga tgaagaaaat gaaactggag
      481 cttacatctt atctagaaga caacttgaaa ctgagaagtc ttatagttat ttacaagaaa
      541 aattagataa taatgaaatc atcgaagcga aagtaacaga agtagttaaa ggtggtttgg
      601 ttgttgatgt aggacaaaga ggttttgttc cggcttcact aatttcaaca gacttcattg
      661 aggatttctc tgtgtttgat ggacaaacaa ttcgtattaa agttgaagaa ttggatcctg
      721 aaaataatag agtcatttta agccgtaaag cagttgaaca agaagaaaac gatgctaaaa
      781 aagatcaatt attacaatct ttaaatgaag gcgatgttat tcatggtaaa gtagcgcgtt
      841 taactcaatt tggtgcattt atagacattg gtggtgttga tggtttagtg catgtatctg
      901 aactttctca cgaacatgtt caaacaccag aagaagtagt ttcaattggt caagatgtta
      961 aagttaaaat taaatctatt gatagagata cagaacgtat ttcattatca atcaaagata
     1021 cgttaccaac acctttcgaa aatattaaag gtcaattcca cgaaaatgat gtcattgaag
     1081 gtgtcgtagt aagattggca aactttggtg catttgttga aattgcacca ggtgtacaag
     1141 gacttgtaca tatttctgaa attgcacaca aacacattgg tacgccaggt gaagtgttag
     1201 aacctggtca acaagtaaat gttaaaatat taggtattga tgaagagaat gaaagagtat
     1261 cactatctat taaagcaaca ttaccaaacg aagatgttgt tgaaagtgat ccttctacga
     1321 ctaaggcgta cttagaaaac gaagaagaag ataatccaac aattggcgat atgattggtg
     1381 ataaacttaa aaatcttaaa ctataattta atatttaata gtcaactcca catgtttatg
     1441 attgcatgtg gagtattttt atgtaacaaa atatactcgg aatgataacg tggacaaatt
     1501 taactaagtg tttaaaaaga taaagtttta agtgctgatt tttatcatta cagtaataaa
     1561 ctcattttga atacacagtc tcatgtgata ttattaaaaa gatataagaa agagaggaag
     1621 ttagcttatg actaaaccta tagtagctat tgtaggtagg cctaatgtag gtaaatctac
     1681 aatttttaat agaatagttg gagaacgtgt ttcgattgtg gaagacacgc caggtgtaac
     1741 acgagatcgt atttattctt caggtgaatg gttaacacat gatttcaata ttattgatac
     1801 aggtggtatt gaaattggtg atgcaccatt ccaaacacaa attagagcgc aggcagaaat
     1861 cgccatagat gaagcggatg ttattatttt tatggttaac gtgcgtgaag gattgacaca
     1921 aagcgatgaa atggtcgctc aaattttata caaatctaaa aaaccggtcg tattagcggt
     1981 taacaaagta gataatatgg aaatgcgtac agacgtgtat gatttctatt cattaggatt
     2041 tggtgaaccg tatccgatat cagggtcaca tggtttaggt cttggtgact tgttagatgc
     2101 agttgtttct cattttggtg aagaggaaga agatccttat gatgaagata caattcgact
     2161 atccattatt ggacgaccaa acgtaggtaa atcaagttta gtaaatgcta ttttaggtga
     2221 agatcgcgtt atcgtttcta atgttgcagg gacaacgaga gacgctattg atacagagta
     2281 tagttatgat ggacaagatt atgttttaat cgatactgct ggtatgcgta aaaaaggaaa
     2341 agtatatgaa tcaactgaga aatattcagt actaagagct ttaaaagcga ttgaacgttc
     2401 aaatgttgtt ttagtggtca ttgatgcaga acaaggcatc attgaacaag ataaacgtgt
     2461 tgcaggatat gcacatgaac aaggtaaagc agtcgtgatt gtcgtaaata aatgggatac
     2521 tgtggaaaaa gatagtaaaa cgatgaagaa atttgaagat gaagtacgta aagaattcca
     2581 atttttagat tatgcacaaa ttgcttttgt gtctgctaaa gaacgcacaa gattacgtac
     2641 attattccct tacattaatg aagcaagtga aaaccataaa aaacgtgttc aaagttcaac
     2701 tttaaatgaa gttgttactg atgcaatttc catgaaccct acaccaacag acaaaggtag
     2761 acgtttgaat gtcttttatg caacacaagt tgctatagaa ccaccgacat ttgttgtatt
     2821 tgttaatgat gtagaattaa tgcatttttc ttataaacgc tatttagaga atcaaatccg
     2881 tgccgctttt ggttttgaag gtacaccaat tcatattata gctcgaaaga gaaattaacg
     2941 attgggggat aacaatgact aaaattaccg tttttggtat gggaagtttt gggacagccc
     3001 ttgccaatgt tcttgcagaa aatggacatg atgttttgat gtggggtaaa aatcaagatg
     3061 ctgttgatga attaaataca tgtcatacaa ataaaaagta tttaaaatac gcgaaattag
     3121 atgttaacat catcgctact tcagatatga ccaaagcaat tcaatttgca gatatttact
     3181 taatggcttt acctactaaa gcaatgcgag aagttgctac tcaaattaat gataagctga
     3241 cctctaaaaa gacttttata catgttgcta aaggtattga aaatgggacg tttaaacgtg
     3301 tgtcagaaat gattgaagat tctatttcac ccgaatataa tgcaggtatt ggcgtgttgt
     3361 cagggccaag tcatgcggaa gaagttgtag tcaagcaacc aactacagtt gctgcatcat
     3421 caaaagataa aagtgtaagt aaattaacgc aagatttatt tatgaatgat tatttgcgtg
     3481 tgtacacgaa tgatgacttg attggtgttg aacttggtgg tgcattgaaa aatatcatcg
     3541 cagtagcaag tggtatcgta gctggaattg gctacggtga taatgcaaaa gctgcgttaa
     3601 tgactcgtgg tttagcggaa attagtagat taggtgaaaa gttaggtgcc gatcctatga
     3661 catttctagg tttaggtggt atcggtgact taatcgttac ttgcatatca acacattctc
     3721 gaaatttcac attaggatat aaacttggac aaggtgaatc aatggatcaa gcattatctg
     3781 aaatgaatat ggttgttgaa ggtatttata caactaaatc agtttatcat ttagctaaag
     3841 aaaaaaatgt ggatatgcca attacaaatg cattatatag agtattattt gaaaatatct
     3901 cagtaaaaga atgcgtaaaa gatttaatgg agcgcgataa aaaatctgaa taaaatgtgt
     3961 attattacac atatacggct aataattacg taaaatgaat agaaatggct taaattcaac
     4021 gtttttgcca cataaatgat tgcgatttat gtaatgcttg tgttaaagtt ttaccataat
     4081 gatataaaca tatcattatc aaacctcagg aggtgaatgt ctaatgaaca aaacagattt
     4141 aatcaatgca gttgcagagc aagctgattt aactaaaaaa gaagctggtt cagcagtaga
     4201 tgctgtattc gaatcaatcc aaaactcact tgctaaaggt gaaaaagtac aattaattgg
     4261 tttcggtaac tttgaggtac gtgaacgtgc tgcacgtaaa ggtcgtaacc ctcaaactgg
     4321 taaagaaatt gatatcccag caagtaaagt tccagcattc aaagctggta aagcattaaa
     4381 agatgctgta aaataatttt acttaaaaag ccctgaataa gggcttttta ttttgctttt
     4441 aatacttaca acctgtacat aatttgtaat gttcttctaa gtttttaatc tttggaacat
     4501 tcattgttta agcgttcaaa atattacatg acataatagt tttgataaaa atagcaacta
     4561 caaaatatac atatgaataa tgagattaat tgttagcgtt gaatttactt aaaaggtaac
     4621 catgtctact atagtatttt acgttattta aaaagatgaa taatgtaaat gaagtaaagg
     4681 ttattatgag aattacaaaa gctacataaa ttactgttag tttaaattga aatttaaaaa
     4741 tgataagtaa tcttaatgct tcaaaagatt ttaatttctt acatataatg ttaatatgaa
     4801 tagtgataat atcgtgaggt gtagacatgg aaacaactgt tagcaaattg gaaagacaaa
     4861 tagaagaaag attaaagggt gtatcagaat atgaatccat taatattaat catcggttag
     4921 gtaagttact tgattcatat gatattccag atgttgccaa agtagcatgc ctcacaattg
     4981 acacatcaat gagacacctt gatgacatta catataatca tttgtctaaa cattccattt
     5041 tgattggaga tttaattagt gcacattttt atacgctact agcagaaatc aatgatttat
     5101 catttcaaaa tgaaattagt aaagcaattg ttgaaattaa tgaattgaaa tcttcattac
     5161 atcatcaagc tttaaatgat tatgaaattt ctcaagcaat agtaaaaatt gaaacacttt
     5221 tcccatatat tacgttatct catttcggta tcaatattga tgagtcggaa atatataatt
     5281 atttatttga agatatgtct gattactatc catcatactt caagaaatat aatcaaagtg
     5341 aagtaaagca ttacttacac gatatccaaa aaagttattt aaaaagtaga ggtaattaag
     5401 catggctgac aataaagcaa ataaagagca agtaca
//
LOCUS       DANBWH010000173         5428 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.179, whole genome shotgun sequence.
ACCESSION   DANBWH010000173 DANBWH010000000
VERSION     DANBWH010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.179"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            23..481
                     /locus_tag="PPY92_002187"
                     /pseudo
     CDS             23..481
                     /locus_tag="PPY92_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226788.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            468..770
                     /locus_tag="PPY92_002188"
     CDS             468..770
                     /locus_tag="PPY92_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626170.1"
                     /translation="MRTNNHITDLDAIELSSGWNYNSKLVTGKNALLSDSLLRDIKVL
                     SSNAKGDADILETELRAVINAMSQNEGKSVDALKNSTTSLKNNLTKLKEQLDKTTH"
     gene            924..1283
                     /locus_tag="PPY92_002189"
     CDS             924..1283
                     /locus_tag="PPY92_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626171.1"
                     /translation="MILRYATNLKLAKELYQASKPKLQEDKGMIELFEHTFGLQKELV
                     KYVGIAEATTAALYSASFINKNISRLASLSTIGILSVAAYKHFEAGQGKKGAQHALDL
                     LGLATLSLLDTFSCNKK"
     gene            complement(1368..1979)
                     /locus_tag="PPY92_002190"
     CDS             complement(1368..1979)
                     /locus_tag="PPY92_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDJ2626172.1"
                     /translation="MYTFDAQSLTARENYKLLIGSIIPRPIAFVTTLNQDASVNAAPF
                     SFFNIVNNHPPMVAIAVQRAGGKRKDTALNIERTGDFVVHITDEDNVQDINETAAPLA
                     YGDSELSRTGLSLLTSTTIKTPGIKDAKMRFECKLSQMILLGDVLDGADLIIGEIVTY
                     HIDDSIYEGDFKINPHALQAVSRLAGNDYAKLGGIFTIKRPEK"
     gene            complement(2058..2534)
                     /locus_tag="PPY92_002191"
     CDS             complement(2058..2534)
                     /locus_tag="PPY92_002191"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HDJ2626173.1"
                     /translation="MVVVYVAGGCLWGVEAFFATIPGIIHTEAGRANGRSSKLDGPYD
                     GYAECVKLHFDDRMLTITDIMNYLFEIIDPYSVNQQGNDIGEKYRTGLYSCVDDHLIE
                     ARQFIERRKDRDQIAVEVLPLSNYIKSAEEHQQHLEKYPEDMHMCHISKDLLNKYK"
     gene            complement(2537..3037)
                     /locus_tag="PPY92_002192"
     CDS             complement(2537..3037)
                     /locus_tag="PPY92_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDJ2626174.1"
                     /translation="MVLRKVIMQDLDQIIALENIGFSPEEAATREALKLRIEQIQETF
                     IVAEKNDEVIGYINGPVIKERYISDDLFKNVSINNSEGGYISVLGLVVAPNYQGQGIA
                     GRLLNYFETLAKNHHRHGVTLTCRESLISFYEKYGYRNEGVSESCHGGIKWYNLVKNI
                     KKDDEV"
     gene            complement(3272..4039)
                     /locus_tag="PPY92_002193"
     CDS             complement(3272..4039)
                     /locus_tag="PPY92_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HDJ2626175.1"
                     /translation="MIDIHNHILVDIDDGPKTIEKSIALLKQAKDEGVTSIVATPHHL
                     HPRYDNTFQQVLVKLAELRTHPEVQALDIKLFPGQEIRITDSILKGLDNGSIQGINRS
                     KYLLIEFPTGEVPHYTKQLFFEIQSRGYIPIIAHPERNRSIAKNPEILYELVANGALS
                     QLTSSSLVGGFGKNIQKLSLQFIECNLAHFVASDAHSCDQRPFLMQELFHNHKLKKYS
                     NDIEALLRNASSVINDNFVYLDRPTKPGKVKSFLKWF"
     gene            complement(4036..4728)
                     /locus_tag="PPY92_002194"
     CDS             complement(4036..4728)
                     /locus_tag="PPY92_002194"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HDJ2626176.1"
                     /translation="MTNTRRSTSSLIVHEQPKSPISEKFRGIRSNIMFANPDSAVQSI
                     VITSEAPGAGKSTIAANLAVAYAQAGYKTLIVDGDMRKPTQHYIFNLPNNEGLSSLLL
                     NWSTYQDSIISTEIQDLDVLTSGPIPPNPSELITSRAFANLYDTLLMNYNFVIIDTPP
                     VNTVTDAQLFSKFTGNVVYVVNSENNNKDEVKKGKELIEATGAKLLGVVLNRMPKDKS
                     ASYYAYYGTDES"
     gene            complement(4745..5407)
                     /locus_tag="PPY92_002195"
     CDS             complement(4745..5407)
                     /locus_tag="PPY92_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapA"
                     /protein_id="HDJ2626177.1"
                     /translation="MKEKFDLVKLLNILKKNIKLLLILPAICLVVSTALTFFVMPDKY
                     TASTQILVNMKKSSSDLAFQNVQSSLQSVNTYTEIIKSPRILDKVSREFDGQYSTAEL
                     NSFLKVTNQTNSQIITVSVTTGNKSESDKIVNRISKVFAHDMPKIMSVDNVTILSSAH
                     DNAVKVSPIVSVNLVISIIVGIVLAILIIFLKELLDKRIKTEEDVESQLGLPILGSIQ
                     KF"
BASE COUNT         1801 a          985 c          781 g         1861 t
ORIGIN      
        1 catgattagt ttataataat aaagtgaaat tgaaaaagac agctattatg cgatgagcga
       61 aaaacttcaa gtaaaacaag aatttaacgg cgttacaagg caatacttga atttcagaac
      121 agatagtatc gatgatctgg ataagttcaa aaaagaattc aaacctgtta tgcacttaaa
      181 aggtgatgca ttcaatcaac agttacaatc tttgattaac aaatatccac aaatacaaaa
      241 aaatatgaaa tcagagttca ttgcttatta tgataaagaa aaaaatagag aaacagtaaa
      301 aaactatgct tggaaccttc aaaaatctat aaatgacatt atgcaatcat atcctagcac
      361 aaaatttgta cagttttata aagatgatgt ttccccgtca atggtagatg gaaatggccg
      421 tttaaaatcg gatactaatg taatttccat cgagggagga aagtatgatg agaacaaata
      481 atcatataac cgatttagat gctattgagt taagtagcgg ttggaattac aattcgaaat
      541 tggtgactgg caaaaatgca ctactatcag atagtctttt aagagatatt aaagtattaa
      601 gttctaatgc taaaggcgac gctgacatac tagaaacaga acttcgtgct gtaataaacg
      661 caatgtctca aaacgaaggt aaatctgttg atgcacttaa aaatagtacc acttctctaa
      721 aaaacaatct aaccaaatta aaagagcaac tcgacaaaac tacacattaa tttcatacat
      781 tttgagtgaa cagaattata tttccatagc aaacattcct aaactacaca tacgttacat
      841 aattgattca tttttataga aacgggtaaa aatgataaag taatcatctt tcacaaaaat
      901 ccaaagattg ggaggaatta aatatgattt tgcgttacgc aacaaattta aaacttgcga
      961 aagagctata ccaagccagt aaacctaaat tgcaagaaga taagggtatg attgaactgt
     1021 ttgaacatac ttttggcttg caaaaagaac ttgtaaaata cgtcggcatt gcagaagcaa
     1081 ctactgctgc tttatactca gctagcttta tcaataaaaa catttctcgc cttgcatcac
     1141 tatcaacgat aggcatttta agtgtagctg cttacaaaca tttcgaagct ggccaaggta
     1201 aaaaaggtgc acaacatgca ttagatttat taggtcttgc tactttaagt ttactagata
     1261 cattttcttg taataaaaaa tagtcttcat tttagaagca catactttag cacctgtgta
     1321 cttttgattc aatccataaa cggtacaccg ctatagcatg tgttttatta tttttctggt
     1381 ctctttatcg taaatattcc ccctaattta gcataatcat ttccggctaa acgagaaaca
     1441 gcctgtaatg cgtgtggatt gattttaaaa tcaccctcat aaatactgtc atcaatatga
     1501 taagttacaa tttcacctat tattaaatca gccccatcta atacatctcc aagcaatatc
     1561 atttgcgata gtttacattc gaatctcatt ttcgcgtctt taattcctgg cgtcttaatc
     1621 gttgtagatg ttaaaagtga taatcctgta cgactcaact cactgtcacc atatgctaac
     1681 ggcgctgcag tctcattaat atcttgaaca ttatcttcgt ctgtaatatg cacaacaaag
     1741 tctccagtcc gttctatatt caaggcagta tcttttctct tacctcctgc acgttgaact
     1801 gcaatagcaa ccattggcgg atggttatta acaatattaa aaaagctaaa tggtgctgca
     1861 tttactgatg catcttgatt taatgttgta acaaatgcta taggtcgtgg aataattgaa
     1921 ccaattaata atttatagtt ttctctagca gttaatgatt gtgcatcaaa cgtatacata
     1981 atacctacct cttttcttag tatatctagg tatttctccg attttggtta atttaaacat
     2041 ctattctcct ctgaaaatca cttgtattta tttagcaaat cttttgaaat atgacacata
     2101 tgcatatctt ctggatattt ttctaaatgt tgctgatgtt cttcagcact tttaatgtag
     2161 ttagacagcg gtaagacttc cactgcaatt tgatctctgt ctttacgtcg ttcaatgaac
     2221 tgacgcgctt caattaagtg gtcatctaca caactatata aacccgttcg atacttttct
     2281 ccaatatcat ttccttgttg attcacactg taaggatcaa tgatttcaaa taaataattc
     2341 ataatgtctg taattgttaa catacgatca tcgaaatgaa gtttgacaca ttcagcataa
     2401 ccatcatacg gaccgtctaa tttagagctt cttccatttg ctcttcctgc ttctgtatgt
     2461 ataattccag gtattgttgc aaaaaatgct tcaacacccc atagacaccc tcctgctaca
     2521 taaacaacta ccatatttac acctcatcat ccttttttat atttttaaca aggttatacc
     2581 atttaatacc gccatgacat gattctgata caccttcatt acgataccca tatttttcat
     2641 aaaatgaaat taatgattct cgacatgtta acgttacacc atgtcgatga tgattcttag
     2701 caagagtttc aaaatagttt agtaagcgac ctgcaatacc ctgaccttga taatttggtg
     2761 ctacaacaag acctaacaca ctaatatagc caccttcact attatttatg gagacatttt
     2821 taaataaatc atcgctaatg taacgctctt ttatgactgg accgttgatg tatccaatta
     2881 cttcatcatt cttttcagct acaataaaag tttcttgaat ttgttcaatt ctcaatttca
     2941 atgcctctcg tgttgcagct tcttccggtg aaaaaccaat attttcaagt gcaataattt
     3001 gatctaagtc ctgcatgatt acttttctta ataccataaa aacacttcct tagccctata
     3061 tatcaaaagt aataccttca tctttatatt cattattttc aaactacctt cactataaaa
     3121 aacgagtctt tttattgaaa gccattcgcc ttaatcaatt agacaagttg tattatacga
     3181 attagtaaat aatcatgatt ataattcatt ttaagcatac tcaaaaagac tggtacatgt
     3241 ataccagtct aattcgaaaa aatatattta gttaaaacca tttcaaaaac gacttcactt
     3301 ttcctggctt agtcggtctg tctaaataaa caaaattatc attgattact gaactggcat
     3361 ttcgaagtaa tgcttcaata tcattcgaat atttcttcaa tttatgattg tgaaataatt
     3421 cttgcatcaa aaatggtctt tggtcacatg aatgtgcatc tgaagctaca aaatgagcca
     3481 aattacattc tataaattgt aatgataact tttgaatgtt tttaccaaat ccaccaacta
     3541 aagaactcga tgttaattga ctcaatgccc catttgcaac caattcatat aatatttctg
     3601 gatttttggc gatacttcta tttctttcag gatgtgcaat gattggtatg tagcctctcg
     3661 attgtatttc aaaaaacaat tgttttgtat aatgtggtac ttcgcccgtt ggaaattcaa
     3721 ttaataaata tttcgaacga ttaatacctt gaatactacc attatctaag cctttcagaa
     3781 tcgaatctgt aattctaatt tcttgcccgg gaaataattt aatatccaat gcttgaactt
     3841 ctggatgcgt tcttaactcc gccaatttca caagcacttg ttgaaatgta ttatcatatc
     3901 tcggatgcaa atgatgaggt gtcgctacaa tacttgttac accttcatcc ttagcttgct
     3961 ttaatagtgc aatactcttt tcaattgttt taggaccatc atctatatca actaatatat
     4021 ggttatgaat atcaatcatg attcatcagt cccataatat gcatagtaac tagcactttt
     4081 atctttaggc attctattta agactacacc taataattta gcacctgttg cttcaataag
     4141 ttcttttcct tttttaactt catctttatt attattttcc gaattaacta cgtagacaac
     4201 attgccggta aactttgaaa ataattgcgc atctgtaact gtgttcactg gtggcgtatc
     4261 gataatgaca aagttataat tcatcaataa tgtgtcatac aaatttgcaa atgcccttga
     4321 tgtaattaac tctgacggat tcggtgggat tggcccagac gtcaagacgt ctaaatcttg
     4381 aatttcagtt gagataatac tgtcttgata agttgaccaa tttagcaata aacttgatag
     4441 gccttcattg tttggcaaat taaaaatata atgctgcgta ggtttacgca tatccccgtc
     4501 tacgattagt gttttataac ctgcttgcgc atatgcaact gctaaatttg ctgcaattgt
     4561 agacttacct gcgcctggtg cctctgaagt gattacaatg ctttgaactg cactgtcagg
     4621 atttgcaaac ataatatttg atcttatgcc tcgaaatttc tcgctaatag gtgactttgg
     4681 ttgttcatgg acaattaaac ttgatgtact tcttcgtgta ttcgtcatgg taattcctcg
     4741 taaattaaaa tttttgtatt gaacctaaaa taggtaatcc tagttgcgat tcaacatctt
     4801 cttctgtctt aatacgctta tctaataatt cttttaagaa aataatcaat attgctaaaa
     4861 caataccaac aataatgctg atcactaagt tgacagatac tattggagat acttttacag
     4921 cattatcatg tgctgaggaa agtatcgtaa cattatcaac actcataatt ttaggcatat
     4981 catgtgcaaa aactttagat attctattaa caattttgtc agattcagat ttattcccag
     5041 ttgtaactga tacagtaata atctgagagt ttgtttgatt ggttactttt aaaaacgaat
     5101 tcaactcagc tgttgaatac tgaccatcaa actctctaga tactttatct agaattcttg
     5161 gacttttgat aatttccgta tatgtattaa cagactgcaa actactttga acattttgga
     5221 aagctaaatc acttgaggac tttttcatgt tcactagtat ttgagtagaa gcagtatatt
     5281 tgtcaggcat aacaaagaag gttaatgccg tacttactac aagacatatt gccggtaaaa
     5341 taagcaataa tttaatattc ttctttagaa tatttaatag ttttactaaa tcaaactttt
     5401 ctttcatggt ttcctccaca taatcaat
//
LOCUS       DANBWH010000174         5418 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.180, whole genome shotgun sequence.
ACCESSION   DANBWH010000174 DANBWH010000000
VERSION     DANBWH010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5418
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.180"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            53..1453
                     /locus_tag="PPY92_002196"
     CDS             53..1453
                     /locus_tag="PPY92_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDJ2626178.1"
                     /translation="MDTSKQFRGDNRLLLGIVLGVITFWLFAQSLVNLVVPLQSTYSS
                     DVGTINIAVSLSALFAGLFIVGAGDVADKFGRVKITYVGLILNVVGSLLIIITPLPAF
                     LIIGRIIQGLSAACIMPSTLAIINEYYIGTRRQRALSYWSIGSWGGSGICTLFGGLMA
                     TYIGWRSIFVVSILLTLLAMYLIKHAPETKAEPIKGMKAEAKKFDVIGLVILVVTMLS
                     LNVIITQTSHFGLVSPLILGLIVVFICSLVGFVYYENKIKHPLVDFSIFKNRGYSGAT
                     ISNFLLNGVAGGALIVINTYYQQQLGFNSSQTGYISLTYLIAVLSMIRVGEKILSQHG
                     PKRPLLLGSGFTVIGLILLSLTFLPEVWYIISSIVGYLLFGTGLGLYATPSTDTAVAS
                     APDDKSGVASGVYKMASSLGNAFGVAVSGTVYTVLAANLNLNLGGFTGMMFNALLAIV
                     AFLVILLLVPKNQTNL"
     gene            1679..2614
                     /locus_tag="PPY92_002197"
     CDS             1679..2614
                     /locus_tag="PPY92_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDJ2626179.1"
                     /translation="MKIAIAGSGALGSGFGAKLFQAGYDVTLIDGYTSHVEAVKQHGL
                     NITINGEAFELNIPMYHFNDQRDESIYDVVFLFPKSMQLKEVMEDMKPHIDNETIVVC
                     TMNGLKHEEVIAQYVAQSQIVRGVTTWTAGLESPGHSHLLGSGPVEIGELVDEGKENV
                     IKVADLLNEAELNGVISKDLYQSIWKKICVNGTANALSTVLECNMASLNESSYAKCLI
                     YKLTQEIVHVATIDNVHLNVDEAFEYLVDLNEKVGAHYPSMYQDLIVNNRKTEIDYIN
                     GAVATLGKQRHIEAPVNRFITDLIHTKESQRHAQD"
     gene            2936..3787
                     /locus_tag="PPY92_002198"
     CDS             2936..3787
                     /locus_tag="PPY92_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDJ2626180.1"
                     /translation="MSKIFVTGATGLIGIKLVQRLKEEGHEVAGFTTSENGQQKLAAI
                     NVKAYIGDILKADTIDQALADFKPEIIINQITDLKNVDMAANTKVRIEGSKNLIDAAK
                     KHDVKKVIAQSIAFMYEPGEGLANEETSLDFNSTGDRKVTVDGVVGLEEETARMDEYV
                     VLRFGWLYGPGTWYGKDGMIYNQFMDGQVTLSDGVTSFVHLDDAVETSIQAIHFENGI
                     YNVADDAPVKGSEFAEWYKEQLGVEPNIDIQPAQPFERGVSNEKFKAQGGTLIYKTWK
                     DGMNPIK"
     gene            complement(3879..5375)
                     /locus_tag="PPY92_002199"
     CDS             complement(3879..5375)
                     /locus_tag="PPY92_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDJ2626181.1"
                     /translation="MTTKKLYFLSISIIILVAISIAIYITLNSNTKTRLTNDSQQQID
                     KIIEHDLQKGHIPGASILIVKNGKVFLNKGYGYQDVDKKVKASPTTKYEIASNTKAFT
                     GLAILKLAQEGRLNLNDDVSKHVPHFKMNYNGQNETITIKQLLAQTSGIPSDITSEDA
                     VTNKNNRLNDVTRAIMGDELHHKPGEEFEYSNMNYDLLGLIIQNVTKQSYTKYITNSW
                     LKPLHMTHTSFKQTNNKSKHDAIGYELQGSTPVVSKPEFNLWDTPSAYMMTSTEDLEH
                     WIKFQLNPPDKYKSLVQQSHKNLSSTIGEPNANAYASGWFTNNDEHLVFHSGTLDNFS
                     SFILLNPKQNYGIVVLANLNSEYVPKLVEHLNTQIVNHKRYSTVASILNQYKDQFNIV
                     TVLMTTLILLAFIFSAYRAWQMRHGQILLRRSKRIAVLSWLTLCLCIAIALILYALPY
                     LILGSNNWSFVLTWLPIEIKLALITTLIALFSTLIVILLFLHTKITKT"
BASE COUNT         1744 a          737 c         1072 g         1865 t
ORIGIN      
        1 tatatcatat taaaaatttg tttttcttaa acataggagg cttatctaat tcatggacac
       61 atcaaaacaa tttagaggtg acaaccgatt gcttttgggt atcgttttag gggttattac
      121 cttttggcta ttcgcgcagt cacttgttaa tcttgttgtc ccattacaat caacatatag
      181 tagtgacgtt ggaacgataa atatcgctgt tagcttatct gccttatttg ctggtttgtt
      241 tatcgtaggt gctggtgatg ttgctgataa atttggtcgc gtcaaaatta cttatgtagg
      301 attgatatta aatgttgtag gttcattact catcatcatt acacctttgc cagcattttt
      361 aattataggt agaataattc aaggtttgtc tgcagcatgt attatgccat ccacacttgc
      421 tattattaac gaatattata ttggtacaag aagacaacgt gccttaagct attggtctat
      481 tggttcttgg ggtggtagtg gtatttgtac gttgtttggt ggcttaatgg ctacatatat
      541 aggttggcgt tcaatatttg ttgtttcaat tctattaaca ttattagcaa tgtacttaat
      601 caaacatgca cctgagacta aagcagaacc aatcaaaggt atgaaagcag aagctaaaaa
      661 gtttgacgtt attggtttag tcattttagt agtgacgatg ttaagtttaa atgtaatcat
      721 cacacagacg tctcattttg gtttagtttc accgttaatt ctaggtttaa ttgttgtgtt
      781 tatctgttca ttagttggtt ttgtatatta cgaaaataaa ataaagcatc cacttgttga
      841 tttttcaatt tttaaaaata gaggatacag tggtgcaact atttcaaact tcttattaaa
      901 tggtgtagca ggtggtgcac ttatcgttat taacacgtat tatcaacaac aattaggatt
      961 taattcttcg caaacgggtt atatttcatt aacgtattta atagcagtgt tgtcaatgat
     1021 tcgtgtaggt gaaaagattt tatctcaaca tggtccgaag cgcccactat tactaggaag
     1081 tggctttaca gtgattgggt taatcttatt gtcgttaaca tttttaccag aagtgtggta
     1141 tatcatatct agtatagttg gatatttatt gtttggtact ggtttaggat tatatgctac
     1201 accatcaact gatacagcag ttgctagtgc gccagatgat aagtcgggtg ttgcttcagg
     1261 cgtatataaa atggcgtcat cattaggaaa tgcatttgga gtagcagtat ctggtacagt
     1321 ttatactgtg ttagcagcta atttaaattt gaacttaggt ggtttcacag gtatgatgtt
     1381 taatgccttg ctagcaattg ttgcattttt agtcatttta ctattagttc ctaaaaatca
     1441 aacgaatttg taaaaccgaa atgaaagcaa gttattatgt agggatttta aaggaaattt
     1501 tgtgaaagta agtttatcat acacacttaa tgttacgtat tgacgtttaa tgttaggtgt
     1561 gttcttttat agacgataaa agctgtgtgc atattaagcg aatgattttc aaattgacgc
     1621 taatatgcga aagtagtatt tttaaaatga acaacaacga tgaagagggg tttataggat
     1681 gaaaattgca attgctggat cgggtgcatt aggtagtggc tttggtgcca aactatttca
     1741 agcaggatat gatgtcacac ttattgacgg atatacatct catgttgaag cggttaagca
     1801 acatggatta aatataacga ttaatggaga ggcattcgag ttaaacattc cgatgtatca
     1861 ttttaatgat caacgggacg aaagcattta cgatgttgtc tttctatttc caaagtctat
     1921 gcaattaaaa gaagtgatgg aagatatgaa gccacatatt gataatgaaa cgatcgtcgt
     1981 atgtacgatg aatggtctga agcatgaaga agtcattgcg cagtatgttg ctcaatcaca
     2041 aattgtcaga ggtgttacga cttggacggc aggtcttgaa agccctggac acagtcattt
     2101 acttggtagt ggaccagttg aaataggtga actagtggat gaaggtaaag aaaatgttat
     2161 aaaagttgct gatttactta acgaagcgga attgaatggt gtcattagta aagatttata
     2221 ccaatcgatt tggaaaaaga tttgtgttaa tggtacggca aatgcattaa gcacagtgtt
     2281 ggagtgtaat atggcatcac tgaatgaaag tagttatgcg aagtgtttga tttataaatt
     2341 aacgcaagaa atagtgcatg tagcgacgat tgataatgtt catttaaatg ttgatgaagc
     2401 atttgaatat ttagttgatt taaatgaaaa agttggtgcg cattatccat ccatgtatca
     2461 agatttaatt gttaataata gaaaaactga aattgattat attaatggcg cagttgcaac
     2521 attgggtaaa caacgtcata ttgaagcgcc agtcaatcgc tttattactg atttaattca
     2581 tactaaagaa agtcagcgac acgcacagga ttaattggtg ttttaggtat gattacaagg
     2641 aaatgacaat cacgtgatat tacggtcatt attaagattg aaatgtaata aataaagaac
     2701 agcagtaagg tactttcaaa ttgaaatgat cttggtgctg tttttcttga ttgatcttcg
     2761 tcataattca gatttgtcat aggtctacga catactatta gtatttacta gacagttttt
     2821 acgacgacac tttgaaaaat tttgaggcaa atcatttgga agtctcacgt gaattttgta
     2881 aactcatcaa gcaagtaatt atattaaaaa gacaaataga gaaaaggtgt ttataatgag
     2941 taaaattttt gtaactggtg caacgggcct tattggcatt aaattagttc aaagactaaa
     3001 agaagagggg catgaggttg ctggttttac tacatctgag aatggtcaac aaaagctagc
     3061 tgctattaat gtaaaagcat atattggtga tatattaaaa gctgatacta ttgatcaagc
     3121 gttagcagat tttaaaccag aaatcattat caaccaaatt acggatttaa aaaatgttga
     3181 tatggcagca aatacgaaag tacgtattga aggttctaaa aacttaattg atgcggcgaa
     3241 aaagcatgac gttaagaaag taattgccca aagtattgcc tttatgtatg aacctggcga
     3301 aggattagca aatgaggaaa cttcacttga ttttaactca actggtgata gaaaagtaac
     3361 ggttgatggt gtggttggtc tagaagaaga aacggctcgt atggatgaat acgttgtttt
     3421 acgttttggt tggttatatg gcccaggtac ttggtacgga aaagatggca tgatttataa
     3481 tcaatttatg gatggtcaag tgacactttc agatggcgta acatcatttg tgcatcttga
     3541 tgatgcagtt gaaacatcta ttcaagctat tcattttgaa aatggtatct ataatgtagc
     3601 agatgatgca cctgttaaag gttctgaatt tgcagaatgg tataaagaac aacttggtgt
     3661 tgaaccaaat attgatattc aacctgcaca accatttgaa cgtggcgtaa gcaatgagaa
     3721 gtttaaagcg caaggtggta ctctgattta taaaacttgg aaagacggca tgaatccaat
     3781 taaataataa tttatccgtt taatatacaa agaataaaga cttggtcgaa tcgtggatga
     3841 tatattatca aacgcacggc tcgaacaagt cttttttatt atgtcttcgt tatctttgta
     3901 tgaaggaata acagaattac aattaatgta ctgaataatg caattaatgt tgtgattagt
     3961 gctaatttaa tttctattgg tagccaagtc agtacaaaag accaattatt gctaccgaga
     4021 atgagatatg gtaatgcata taatatgagc gctatagcga tacataaaca taaagttagc
     4081 caactcaata cagcaatccg ttttgatcta cgcaaaagaa tttgaccatg gcgcatttgc
     4141 caagcacgat aagctgagaa tataaatgct aataaaataa gtgttgtcat caaaacggta
     4201 acaatattaa attggtcttt atattgattg agtatagacg caaccgtcga atatcgcttg
     4261 tgatttacaa tttgtgtatt aagatgctca actaatttgg gtacatattc cgaatttaga
     4321 tttgcaagca caacaattcc ataattttgt tttggattta gtaaaataaa tgatgaaaag
     4381 ttatcgagcg ttcctgaatg aaacactaaa tgttcatcat tattggtaaa ccagcccgaa
     4441 gcatatgcat tggcattagg ttcaccaatt gttgatgata aatttttatg tgattgttga
     4501 actaatgatt tgtatttatc aggtggatta agttggaatt ttatccaatg ttccaaatct
     4561 tcagttgatg tcatcatata tgctgatggt gtatcccaaa ggttaaattc aggtttagag
     4621 acgacaggtg tcgaaccttg taattcgtag ccaatagcat catgttttga tttgttattg
     4681 gtttgtttga atgatgtatg tgtcatatgc aaaggcttga gccatgaatt tgtaatatat
     4741 tttgtatagg attgcttcgt aacgttttgg ataattaaac ctaataaatc atagttcata
     4801 tttgagtatt caaattcttc tccgggctta tgatgtaatt catcacccat aattgcacgg
     4861 gttacatcat ttaaacgatt atttttattt gttacggcgt cttcgcttgt aatatcacta
     4921 ggtataccac ttgtttgagc caaaagttgc ttaatcgtaa tagtttcatt ttgaccatta
     4981 tagttcattt taaaatgagg cacatgtttg gatacgtcat catttaagtt taatcgacct
     5041 tcttgagcta attttaaaat tgcaagacct gtgaaagctt tagtattaga agcaatttca
     5101 tactttgttg tgggagaagc tttgactttt ttatcaacat cttgataacc ataaccttta
     5161 tttaaaaaaa ctttgccatt ttttactatt aaaattgatg ctccaggaat gtgtcccttt
     5221 tgtaaatcat gctcgataat tttatctatt tgttgttgcg aatcattggt taaccgtgtc
     5281 ttcgtattgc tatttaatgt tatatatata gcaattgaaa tggcgactaa aatgataata
     5341 gaaatggata gaaaatacag ttttttagta gtcataaacg ttccttttta tatgttaaat
     5401 ttacttaagt gcctaatg
//
LOCUS       DANBWH010000175         5385 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.181, whole genome shotgun sequence.
ACCESSION   DANBWH010000175 DANBWH010000000
VERSION     DANBWH010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.181"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(109..948)
                     /locus_tag="PPY92_002200"
     CDS             complement(109..948)
                     /locus_tag="PPY92_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDJ2626182.1"
                     /translation="MKKTLTVTVSSVLAFLALNNAAHAQQHGTQVKTPVQHNYVSNVQ
                     AQTQSPTTYTVVAGDSLYKIALEHHLTLNQLYSYNPGVTPLIFPGDVISLVPQNKVKQ
                     TKAVKSPVRKASQAKKVVKQPVQQASKKVVVKQAPKQAVAKTVNVAYKPAQVQKSVPT
                     VPVAHNYNKSVANRGNLYAYGNCTYYAFDRRAQLGRSIGSLWGNANNWNYAAKVAGFK
                     VDKTPEVGAIFQTAAGPYGHVGVVESVNPNGTITVSEMNYAGFNVKSSRTILNPGKYN
                     YIH"
     gene            complement(1096..1203)
                     /locus_tag="PPY92_002201"
     CDS             complement(1096..1203)
                     /locus_tag="PPY92_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626183.1"
                     /translation="MCLLKQHMQCAFSNITYGDFQGIELIVENVLNYMV"
     gene            complement(1357..2467)
                     /gene="prfB"
                     /locus_tag="PPY92_002202"
     CDS             complement(join(1357..2394,2396..2467))
                     /gene="prfB"
                     /locus_tag="PPY92_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667688.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDJ2626184.1"
                     /translation="MELSEIKRNIDKYNQDLTQIRGSLDLENKETNIQEYEEMMAEPN
                     FWDNQTKAQDIIDKNNALKAIVNGYKTLQAEVDDMDATWDLLQEEFDEEMKEDLEQEV
                     INFKAKVDEYELQLLLDGPHDANNAILELHPGAGGTESQDWANMLFRMYQRYCEKKGF
                     KVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFAS
                     CDVIPDFNNDEIEIEINPDDITVDTFRASGAGGQHINKTESAIRITHHPSGIVVNNQN
                     ERSQIKNREAAMKMLKSKLYQLKLEEQAREMAEIRGEQKEIGWGSQIRSYVFHPYSMV
                     KDHRTNEETGKVDAVMDGDIGPFIESYLRQTMSHD"
     gene            complement(2636..2740)
                     /locus_tag="PPY92_002203"
     CDS             complement(2636..2740)
                     /locus_tag="PPY92_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626185.1"
                     /translation="MLWEESFYFTSVKYFKKCEEVVKRCYVLSFKKSV"
     gene            complement(2782..5313)
                     /gene="secA"
                     /locus_tag="PPY92_002204"
     CDS             complement(2782..5313)
                     /gene="secA"
                     /locus_tag="PPY92_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDJ2626186.1"
                     /translation="MGFLSKILDGNNKEIKQLGKLADKVIALEEKTAILTDEEIRNKT
                     KQFQTELADIDNVKKQNDYLDKILPEAYALVREGSKRVFNMTPYKVQIMGGIAIHKGD
                     IAEMRTGEGKTLTATMPTYLNALAGRGVHVITVNEYLSSVQSEEMAELYNFLGLTVGL
                     NLNSKTTEEKREAYAQDITYSTNNELGFDYLRDNMVNYSEDRVMRPLHFAIIDEVDSI
                     LIDEARTPLIISGEAEKSTSLYTQANVFAKMLKQDEDYKYDEKTKAVHLTEQGADKAE
                     RMFKVENLYDVQNVDVISHINTALRAHVTLQRDVDYMVVDGEVLIVDQFTGRTMPGRR
                     FSEGLHQAIEAKEGVQIQNESKTMASITFQNYFRMYNKLAGMTGTAKTEEEEFRNIYN
                     MTVTQIPTNKPVQRNDKSDLIYISQKGKFDAVVEDVVEKHKAGQPVLLGTVAVETSEY
                     ISNLLKKRGIRHDVLNAKNHEREAEIVAGAGQKGAVTIATNMAGRGTDIKLGEGVEEL
                     GGLAVIGTERHESRRIDDQLRGRSGRQGDKGDSRFYLSLQDELMIRFGSERLQKMMSR
                     LGLDDSTPIESKMVSRAVESAQKRVEGNNFDARKRILEYDEVLRKQREIIYNERNSII
                     DEEDSSQVVDAMLRSTLQRSINYYINTADDEPEYQPFIDYINDIFLQEGDITEDDIKG
                     KDAEDIFEVVWAKIEAAYQSQKDILEEQMNEFERMILLRSIDSHWTDHIDTMDQLRQG
                     IHLRSYAQQNPLRDYQNEGHELFDIMMQNIEEDTCKFILKSVVQVEDNIEREKTTEFG
                     EAKHVSAEDGKEKVKPKPIVKGDQVGRNDDCPCGSGKKFKNCHGK"
BASE COUNT         1559 a         1043 c          784 g         1999 t
ORIGIN      
        1 tatcatttaa atatcttctt tatataactc tgattaaatg ataccaaaaa atcctctcaa
       61 cctgttactt aaacaggcta agaggatagt cttgtcttga tatattactt agtggatgta
      121 attatatttt cctggattta aaattgttct tgaagattta acattaaatc cagcatagtt
      181 catttccgaa acagtaattg ttccattagg gtttacagat tcaacaacac caacatgtcc
      241 atatgggcca gcagctgttt ggaaaatagc gccaacttct ggtgttttat ctactttaaa
      301 tcctgcaact tttgctgcgt aattccagtt atttgcattg ccccataaac ttcctatact
      361 tctacctaat tgtgcacgac gatcgaaggc ataatatgtg cagtttccat aagcatataa
      421 gtttcctctg ttagcaactg atttattgta gttatgtgca acaggtacag ttggtactga
      481 tttttgtact tgagcaggtt tatatgctac attaactgtc ttagctactg cttgcttagg
      541 tgcttgctta actactactt ttttagacgc ttgttgtaca ggttgtttta ctaccttttt
      601 agcttggctt gcttttctta ctggtgattt aaccgcttta gtttgtttca ctttattttg
      661 aggcacaagt gaaatcacgt caccaggaaa aattaaaggt gttacaccag gattgtatga
      721 atataattga ttcaacgtta agtgatgctc taaagcaatc ttatataatg aatcgccagc
      781 aactactgta taagttgtcg gtgattgcgt ttgtgcttga acatttgata catagttatg
      841 ttgaacaggt gtttttactt gtgtgccatg ttgttgtgca tgtgctgcat tatttaaagc
      901 taaaaaagct aacactgacg aaaccgtcac tgtaagagtt tttttcatct tgctgtcatt
      961 cctttgctgt tagtatttta agtatgcaaa tactatagca caatacattt tgtcaaaagc
     1021 tattgttata acgatgtaat caaatggtta acaatataaa aagaatacaa ccttttatca
     1081 tagtgtaaaa tgtattcata ccatgtaatt gagaacgttt tcaacgatta attcaatacc
     1141 ttgaaaatcg ccataggtaa tattactaaa tgcacactgc atatgttgtt ttaacaaaca
     1201 caacttttaa aaaatatatt ctaactctat ctaccgaatt gtacttaaat attcataaac
     1261 aaatcatatt ccaaaatcta atttacaatt tatttagcta cctttaaaaa aaccaaaaac
     1321 cgacgccctt ttagagcctc ggttttaaaa tatatattaa tcgtgcgaca ttgtctgtct
     1381 taaatatgat tcgataaatg gtccaatgtc tccatccatc actgcatcaa ccttacctgt
     1441 ttcttcgttc gtacgatgat ctttcaccat tgagtatgga tggaaaacat atgatctaat
     1501 ttggcttccc cagccgattt ctttttgttc gccacgaatt tcagccattt cacgtgcctg
     1561 ctcttccaat tttaattgat ataatttaga ctttaacatt ttcatagctg cttcacggtt
     1621 tttaatttga gaacgttcat tttggttatt aacaactata cctgaggggt ggtgggtaat
     1681 tcgtattgcc gattcagttt tgttaatatg ctgaccacct gcaccagaag ctctgaatgt
     1741 atcaactgta atatcatccg gattgatttc aatctctatt tcatcattat taaaatctgg
     1801 aataacgtcg catgatgcaa atgatgtatg acgacgtcct gatgaatcaa atggagaaat
     1861 tcgtactagt cggtgtacac ctttttcagc ttttaaataa ccataagcat tatgcccttt
     1921 gatgagcaat gttacacttt taatccccgc ttcatcccca ggtagataat caacagtttc
     1981 aactttaaag cctttcttct cacaataacg ttgatacatt ctaaatagca tattagccca
     2041 atcttgagac tccgtgccac ctgcaccagg atgtaactct agaattgcgt tattggcatc
     2101 gtgaggccca tctaataata attgcaattc gtattcatcc actttagcct taaaattaat
     2161 gacctcttgc tctaagtctt ctttcatttc ttcatcaaat tcttcttgta ataaatccca
     2221 agtagcatcc atgtcatcta cttctgcttg tagtgtttta taaccattaa ctattgcttt
     2281 taacgcatta tttttatcta taatatcttg cgctttcgtt tggttatccc aaaaattagg
     2341 ttctgccatc atttcttcat attcttggat attagtttct ttgttctcta agtcaaagag
     2401 accccctaat ttgtgttaaa tcttgattat acttatctat atttcgtttg atttctgata
     2461 attccatagc cttcgctcct atttaaattt caattcaagt cattgatttg tatcttttat
     2521 aatgctaaat tttaacataa ttttgttaaa taacaatgtt aagaaatata agcacactga
     2581 caattagttt atgcttttat tgttaaaaat cagtacattt atcatcgaca tatgcctaaa
     2641 ccgatttttt aaaactaagt acataacaac gtttaacaac ttcttcacat tttttaaagt
     2701 atttaacgct tgtaaaataa aaagactcct cccataacac aaactatagg tgtttaattg
     2761 gaaggagtta ttttatatca tttattttcc atggcaattt ttgaattttt taccactacc
     2821 acatggacaa tcatcgttac gaccaacttg atcgccttta acgattggtt tcggtttcac
     2881 tttttcttta ccatcttcag ctgaaacgtg cttcgcttca ccaaactctg ttgttttttc
     2941 acgttcaata ttatcttcaa cttgtactac agattttaaa atgaatttac aagtatcttc
     3001 ttcgatattt tgcatcatga tatcaaataa ttcatgacct tcattttgat agtcacgtaa
     3061 tggattttgc tgtgcataag aacgtaagtg aataccttga cgtaattgat ccattgtgtc
     3121 gatatgatca gtccaatggc tatcaataga acgaagtaaa atcatacgct caaactcatt
     3181 catttgttct tctaagatat ctttttgact ttgatatgct gcttcaatct tagcccaaac
     3241 gacttcgaaa atatcttcag catctttacc tttgatatca tcctctgtaa tgtcaccttc
     3301 ttgtaagaaa atgtcattaa tgtagtcgat gaatggttga tattcaggct cgtcatctgc
     3361 tgtattaata tagtaattga tactacgttg taacgttgaa cgtagcattg catctacaac
     3421 ttgagagctg tcttcttcat caataatact atttctttcg ttatagataa tttcacgttg
     3481 tttacgtaat acttcatcgt attctaagat acgtttacgc gcgtcgaagt tattaccttc
     3541 tacacgtttt tgtgctgatt ctacagctct tgataccatt tttgattcaa ttggtgtaga
     3601 gtcatctaaa cctagtcggc tcatcatttt ctgtaaacgt tcagaaccaa aacgaatcat
     3661 taattcatct tgtaatgata aatagaagcg actatcccct ttatcacctt gacgtccaga
     3721 acgaccacgt aactggtcat caatacgacg agattcatgt cgttctgtac ctattactgc
     3781 taaaccgcct aattcctcta cgccttcacc taatttgata tctgtaccac gaccagccat
     3841 gttagtggca atagtaacgg cacctttttg tccagcgcct gcaacaattt cagcttcacg
     3901 ttcatgattt ttcgcattta acacatcatg acggatacca cgttttttaa gtaaatttga
     3961 aatatattca gaagtctcaa ctgcaacagt acctaatagc actggttgcc ctgccttgtg
     4021 tttttcaaca acatcttcta ctactgcatc aaatttacct ttttggctaa tgtaaattaa
     4081 atcagactta tcgttacgtt gcacaggttt atttgtcgga atttgagtta ctgtcatgtt
     4141 ataaatattt ctaaattctt cttcttcagt tttagctgta cctgtcatac ccgcaagttt
     4201 attgtacatt ctgaaatagt tttggaatgt aatagacgcc atagttttag attcattttg
     4261 aatttgaacg ccttccttcg cttcaatagc ttggtgtaaa ccttctgaga aacgacggcc
     4321 tggcattgta cgtcctgtaa attgatcgac aattagtact tcgccatcaa caaccatata
     4381 gtctacgtca cgttgtaatg taacgtgcgc acgtaaagct gtgttgatat gactaataac
     4441 atcaacattt tgtacatcat ataagttttc aactttgaac atacgttcag ctttatccgc
     4501 accttgttct gttaaatgta cggctttcgt tttttcatcg tatttataat cttcgtcctg
     4561 ttttaacatt ttcgcaaaaa catttgcttg tgtataaagt gacgttgact tttcagcttc
     4621 accagaaata attaatggcg tacgtgcctc gtcgattaaa attgagtcaa cctcatcaat
     4681 gattgcaaaa tgtaatggac gcattactct atcttcagaa taattcacca tgttatctcg
     4741 taagtaatca aaacctagct cattattagt actgtaagta atgtcttgtg cgtatgcttc
     4801 acgtttttct tctgtcgtct tactgtttaa gtttaatccg acagtcaaac ctaagaagtt
     4861 atataactca gccatttctt cactttgaac acttgataag tattcattga ctgtaataac
     4921 gtgaacacct ctaccagcta atgcatttaa gtatgttggc attgtcgctg ttaatgtttt
     4981 accttcacct gttctcatct cagcgatatc acctttatga attgcaatac cacccataat
     5041 ttgaacttta tatggtgtca tattgaatac acgtttagag ccttctctaa caagtgcata
     5101 tgcttctggt aaaattttat ctaaataatc attttgcttt ttgacattat caatgtcagc
     5161 taattctgtt tggaattgtt tcgttttatt acgaatttct tcatcagtta aaattgccgt
     5221 tttttcttct aaagcgatta ctttatcagc aagtttacct aactgtttaa tttctttatt
     5281 attgccatca agaatttttg ataaaaatcc catttcgttc gctcctttag ctaaaaaact
     5341 gtttggccta caacaatata tcttatcatt tatagttaga aaatt
//
LOCUS       DANBWH010000176         5307 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.182, whole genome shotgun sequence.
ACCESSION   DANBWH010000176 DANBWH010000000
VERSION     DANBWH010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.182"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            44..1162
                     /gene="ald"
                     /locus_tag="PPY92_002205"
     CDS             44..1162
                     /gene="ald"
                     /locus_tag="PPY92_002205"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HDJ2626187.1"
                     /translation="MLVAVVKELKQGEGRVACTPENVRKLTDAGHKVIVEKNAGIGSG
                     FSNDMYEKEGAKIVTHEQAWEADLVIKVKEPHESEYQYFKKNQIIWGFLHLASSKEIV
                     EKMQEVGVTAISGETIIKNGKAELLAPMSAIAGQRSAIMGAYYSEAQHGGQGTLVTGV
                     HENVDIPGSTYVIFGGGVAATNAANVALGLNAKVIIIELNDDRIKYLEDMYAEKDVTV
                     VKSTPENLAEQIKKADVFISTILIPGAKPPKLVTREMVKSMKKGSVLIDIAIDQGGTI
                     ETIRPTTISDPVYEEEGVIHYGVPNQPGAVPRTSTMALAQGNIDYILEICDKGLEQAI
                     KDNEALSTGVNIYQGQVTNQGLASSHDLDYKEILNVIE"
     gene            1257..2297
                     /gene="tdcB"
                     /locus_tag="PPY92_002206"
     CDS             1257..2297
                     /gene="tdcB"
                     /locus_tag="PPY92_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HDJ2626188.1"
                     /translation="MTTNTVTLQTAHIVSLGDIEEAKASIKPFIRRTPLIKSMYLSQN
                     ITKGNVYLKLENMQFTGSFKFRGASNKINHLSDEQKAKGIIGASAGNHAQGVALTAKL
                     LGIDATIVMPETAPIAKQNATKGYGAKVILKGKNFNETRLYMEELAKENGMTIVHPYD
                     DKFVMAGQGTIGLEILDDIWNVNTVIVPVGGGGLIAGIATALKSFNPSIHIIGVQAEN
                     VHGMAESFYKRALTEHREDSTIADGCDVKVPGEKTYEVVKHLVDEFILVSEEEIEHAM
                     QDLMQRAKIITEGAGALPTAAILSGKIDKKWLEGKNVVALVSGGNVDLTRVSGVIEHG
                     LNIADTSKGVVG"
     gene            2328..3650
                     /locus_tag="PPY92_002207"
     CDS             2328..3650
                     /locus_tag="PPY92_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDJ2626189.1"
                     /translation="MSNGKELQKNIGFFSAFAIVMGTVIGSGVFFKISNVTEVTGTAG
                     MALFVWFLGGIITICAGLTAAELAAAIPETGGLTKYIEYTYGDFWGFLSGWAQSFIYF
                     PANVAALSIVFATQLINLFHLSIGSLIPIAIASALSIVLINFLGSKAGGILQSVTLVI
                     KLIPIIVIVIFGIFQSGDITFSLIPTTGNSGNGFFTAIGSGLLATMFAYDGWIHVGNV
                     AGELKNPKRDLPLAISVGIGCIMAVYLLINATFLLTLPIELLAGNLNAASDTSKILFG
                     ENGGKIITIGILISVYGTINGYTMTGMRVPYAMAERKLLPFSHLFAKLTKSGAPWFGA
                     IIQLIIAIIMMSMGAFDTITNMLIFVIWLFYCMSFVAVIILRKREPNMERPYKVPLYP
                     IIPLIAILAGSFVLINTLFTQFILAIIGILITALGIPVYYYKKKQKAA"
     gene            3806..5197
                     /gene="norB"
                     /locus_tag="PPY92_002208"
     CDS             3806..5197
                     /gene="norB"
                     /locus_tag="PPY92_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorB"
                     /protein_id="HDJ2626190.1"
                     /translation="MEKPSREAFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSF
                     NTDIGTVNIAVSITALFSGMFVVGAGGLADKYGRIKLTNIGIILNILGSLLIIISNIP
                     LLLIIGRLIQGLSAACIMPATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGA
                     VATLLGWRWIFILSIIISLIALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLLTLNI
                     LITKGSELGVTSLLFITLLAIAIGSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASN
                     FLLNGVAGTLIVANTFVQRGLGYSSLQAGSLSITYLVMVLIMIRVGEKLLQTLGCKKP
                     MLIGTGVLIVGECLISLTFLPEILYVICCIIGYLFFGLGLGIYATPSTDTAIANAPLE
                     KVGVAAGIYKMASALGGAFGVALSGAVYAIVSNMTNIYTGAMIALWLNAGMGILSFVI
                     ILLLVPKQNDTQL"
BASE COUNT         1831 a          758 c         1022 g         1696 t
ORIGIN      
        1 aataattgtg aaattattca caaatgaaag aggagtgttg caaatgttag ttgcagtagt
       61 caaagaatta aaacaaggtg aaggacgtgt agcttgcaca cccgaaaatg tgcgtaagtt
      121 aacggatgca ggacataaag taattgttga aaaaaatgct ggcattggtt caggattttc
      181 taacgatatg tatgaaaaag aaggcgctaa gatcgtaact cacgaacaag catgggaagc
      241 tgatcttgtt atcaaagtaa aagaacctca tgaaagcgaa tatcaatatt tcaaaaagaa
      301 tcaaattatc tggggatttt tacatctagc atcttcaaaa gaaatagtag aaaaaatgca
      361 agaagttggt gtaactgcga ttagtggtga aaccattata aaaaatggaa aagcagaatt
      421 attagcgcca atgagtgcta tagcaggtca acgctcagca attatgggag cttactactc
      481 tgaagcacaa catggtggtc aaggtacttt agtgactggt gtacatgaaa atgtggatat
      541 acctggtagt acatatgtga ttttcggtgg tggcgtagca gcaacaaatg cagcaaatgt
      601 tgccttggga ctaaatgcta aagtaatcat tatcgagtta aacgatgacc gcattaaata
      661 tcttgaagat atgtatgcag aaaaagatgt cacagtagtc aaatcaacac cagaaaattt
      721 agcagaacaa attaagaaag cagatgtatt tatttctaca attttaattc caggtgcgaa
      781 accgccaaaa ttggttactc gtgagatggt taaatcaatg aaaaaaggtt cagtattaat
      841 cgatatagct attgaccaag gtggaactat tgaaacaatt agaccaacta caatttctga
      901 tccagtgtat gaagaagaag gtgtgattca ttatggtgta ccaaatcaac caggagcagt
      961 cccaagaact tcaacaatgg cattagcaca aggaaatatt gattatatat tagaaatttg
     1021 tgacaaaggc ttagaacaag caattaaaga taatgaagcc ttaagtactg gtgtaaacat
     1081 ttaccaagga caagtgacaa atcaaggatt agcttcatca catgacctag attataaaga
     1141 aatattaaat gttatcgaat agataataat ttaaatgaaa ttgagtgaaa tgaatatttt
     1201 aaatatagca ttatagtttg gactaaaaat ttacaaaacg gaaggatgta attattatga
     1261 caaccaacac agttacatta caaacagcac atattgtaag tttaggagat atcgaagaag
     1321 ctaaagcaag cattaaacca tttattcgtc gaacaccact aattaaatct atgtatttaa
     1381 gtcaaaatat cactaaaggt aacgtatatt taaaacttga aaatatgcag tttacaggtt
     1441 catttaaatt tagaggtgct agcaataaaa ttaatcattt gtcagatgaa caaaaagcta
     1501 aaggtattat tggcgcatca gccggaaatc atgcacaagg tgtagctttg acagcaaaac
     1561 tattaggtat tgatgcaacg attgtgatgc cagaaacagc accaattgct aaacagaatg
     1621 ctaccaaagg atatggtgca aaagtcattt taaaaggtaa aaactttaat gaaactagac
     1681 tttatatgga agaattagcg aaagaaaatg gtatgacaat tgttcatcca tatgatgata
     1741 agtttgtcat ggctggtcaa ggaacaatag gattagaaat tttagatgat atatggaatg
     1801 ttaatacggt cattgttcca gttggtggag gtggcctaat tgcaggtatt gccaccgctt
     1861 taaaatcatt caacccatca attcatataa tcggcgttca agctgaaaat gtgcatggca
     1921 tggcagagtc attctataag agagctttaa ctgaacatcg tgaagatagc acaattgcag
     1981 atggttgtga tgtaaaagta ccaggtgaaa aaacatacga agtagtaaaa catttagtag
     2041 atgaatttat tcttgtatca gaagaggaaa tagaacatgc aatgcaagat ttaatgcaac
     2101 gtgccaaaat cattacagaa ggtgcaggag ctttacctac agcagctatt ttaagtggga
     2161 aaattgataa aaaatggcta gagggtaaaa atgtcgttgc attagtttca ggaggaaacg
     2221 ttgacttaac tagagtttct ggtgtcattg aacatggctt aaatatcgcg gatacaagta
     2281 agggtgtggt aggttaaaac ttccaatctt aaaaatgagg tgtaattatg tcaaatggta
     2341 aagaattaca aaaaaatata ggtttcttct cagcgtttgc tattgttatg gggacagtta
     2401 ttggttcagg agtattcttt aaaatatcaa acgtaacaga agtaacagga acagcaggaa
     2461 tggccttgtt tgtatggttc ctaggcggca tcattaccat ttgtgcgggg ttaacagcag
     2521 cagaacttgc tgctgcaatc cctgaaacag gtggcttaac gaagtatata gaatatacat
     2581 acggtgattt ctggggcttc ctatcaggtt gggcgcaatc atttatttat tttccagcta
     2641 acgtagcagc attgtctatc gtatttgcga cacagctaat taatttattc catttatcta
     2701 taggttcgtt aataccaata gcaatcgcat ctgcgttatc tattgtgttg ataaatttcc
     2761 taggttcaaa agcaggcgga attttacaat cagttacttt agtaattaaa ctgattccaa
     2821 tcatcgttat tgtaattttt ggtatttttc aatctggaga tatcactttt tcattaattc
     2881 caactacagg taattcagga aatggtttct ttacagcaat tggtagtggt ttattagcaa
     2941 ctatgtttgc atatgatggt tggattcatg taggaaatgt tgcgggggaa cttaaaaatc
     3001 ctaaacgcga tttaccttta gcgatttcag ttggtatcgg ttgtattatg gctgtgtatt
     3061 tattaattaa cgctacattt ttattaacgt tgccaataga actacttgct ggtaacttaa
     3121 atgcagcttc agatacatca aaaatattat ttggtgaaaa tggcggtaaa attattacaa
     3181 tcggtatatt aatttctgtt tatggtacga tcaatggcta tactatgact ggtatgcgcg
     3241 taccatatgc aatggctgaa agaaaattat tgccatttag ccacttattc gcaaaattaa
     3301 caaaatctgg cgcaccatgg tttggcgcaa ttatacaact tataatcgct atcatcatga
     3361 tgtcaatggg agcatttgat acaataacaa acatgttaat ctttgttatt tggttgttct
     3421 attgtatgtc atttgttgct gtaataattt taagaaaacg tgaaccaaat atggagcgtc
     3481 catataaagt accgttatat ccgatcatac ctttaattgc tattttggca ggatcatttg
     3541 tattgattaa tacactgttt acacaattta tattagcaat cattggaatt ctaataacag
     3601 cacttggtat accagtttat tactataaga agaaacaaaa agcagcataa ggtaagataa
     3661 ctagcattga gaataaatgg atggactact aataaattta aagttttaca cattaaaatc
     3721 aaaaaccatt caattattct atcgaacaga caaatttctg ttatggaatt tgtctgtttt
     3781 caaaagtata gggaggcaaa tagagatgga aaagccgtca agagaggcat ttgaaggcaa
     3841 taataagttg ttaataggaa ttgttttaag tgtaataacg ttttggctat ttgcacaatc
     3901 attggttaat gttgtaccaa tacttgaaga tagtttcaat acagatattg gaacggttaa
     3961 tatcgccgtt agtataactg ctttattttc aggaatgttt gttgtaggag caggtggtct
     4021 tgctgataaa tatggcagaa ttaaactcac gaacattggt attatcttaa atatattggg
     4081 ttcattatta atcattattt caaatattcc tttattactt attataggaa gattaattca
     4141 aggactttca gcagcatgta ttatgcctgc aactttgtct attattaagt catattacat
     4201 tgggaaagat agacaacgcg ctttaagtta ttggtcaatt ggctcatggg gcggctctgg
     4261 tgtttgttca ttttttggag gtgcagttgc aacactttta ggttggcgtt ggattttcat
     4321 cctatcaatt ataatttcat taattgcgct gtttcttatt aaaggcacac ctgaaactaa
     4381 atctaaatcg atttctctaa ataaatttga cattaaaggt ctggttcttt tagtcattat
     4441 gctcctcact ttaaatattt taattactaa aggatcagaa ttaggtgtaa cctcacttct
     4501 ttttatcact ttattagcaa ttgcaattgg atcttttagt ttatttatag ttcttgaaaa
     4561 gcgtgctaca aatcctttaa tcgattttaa attatttaaa aataaagctt atacaggtgc
     4621 aacagcttca aactttttgt taaatggtgt tgcgggaaca ttaatagtag ccaacacatt
     4681 tgttcaaaga ggtttaggat attcctcatt gcaagcagga agtttatcaa tcacttattt
     4741 agtaatggta ctaattatga ttcgtgttgg tgaaaagtta cttcaaacac tcggatgcaa
     4801 gaaaccaatg ttaattggaa caggagttct tattgtcgga gaatgtctca tttcattaac
     4861 tttcttgcca gaaatattgt atgtcatttg ttgtattata ggttatttat tctttggttt
     4921 aggactagga atatatgcta caccatcaac agatacagca attgcaaatg caccgttaga
     4981 aaaagtagga gttgctgcag gtatctataa aatggcttct gcattaggtg gagcatttgg
     5041 cgtcgcattg agtggtgcag tttatgcaat cgtatcaaat atgacaaaca tttatactgg
     5101 tgcaatgatt gcattatggt taaacgcagg tatgggaata ttatcattcg ttatcatttt
     5161 gttacttgtg cctaaacaaa acgacactca attatgataa ttgagaatta aattgaaatc
     5221 atacaagtcg ctacaatatt aaacaaaaat ataaaccgat tcttatgtgt cattatttta
     5281 aatgaacata gggattggtt ttttatt
//
LOCUS       DANBWH010000177         5269 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.183, whole genome shotgun sequence.
ACCESSION   DANBWH010000177 DANBWH010000000
VERSION     DANBWH010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.183"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            189..1076
                     /locus_tag="PPY92_002209"
     CDS             189..1076
                     /locus_tag="PPY92_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDJ2626191.1"
                     /translation="MKKSLIAFILIFMLVLSGCGMKDNDKQGSDDNGSSKSPYHRIVS
                     LMPSNTEILYELGLGKYIVGVSTVDDYPKDVKEGKKQFDALNLNKEELLKAKPDLILA
                     HESQKATANKVLSSLEKQGIKVVYVKDAQSIDETYNTFKQIGKLTHHDKQAEQLVEET
                     KDNIDKVIDSIPAHHKKSKVFIEVSSKPEIYTAGKHTFFNDMLEKLEAQNVYSDINGW
                     NPVTKESIIKKNPDILISTEAKTRSDYMDIIKKRGGFNKINAVKNTRIEVVNGDEVSR
                     PGPRIDEGLKELRDAIYRK"
     gene            1225..2175
                     /locus_tag="PPY92_002210"
     CDS             1225..2175
                     /locus_tag="PPY92_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDJ2626192.1"
                     /translation="MTFNKVLLSWIVILIITTSIYLFWQLGDINDVFNQSILINVRLP
                     RLLEALLTGMILTVAGLIFQTVLNNALADSFTLGLASGATFGSGLALFLGLTTLWIPV
                     FSITFSLITLITVLVITSVLSQGYPVRILILSGLMIGALFNSLLYFLILLKPRKLNTI
                     ANYLFGGFGDAEYSNVSIIAITFIIALFGIFIILNQLKLLQLGELKSQSLGLNVQLIT
                     YIALCIASMITAINVAYVGIIGFIGMVIPQLIRKWQWKQSLGRQLALNIVIGGQIMVM
                     ADFIGSHILSPVQIPASIIIALIGIPVLFYMLISQSKRLH"
     gene            2230..2949
                     /locus_tag="PPY92_002211"
     CDS             2230..2949
                     /locus_tag="PPY92_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDJ2626193.1"
                     /translation="MDLNQIKAVVFDLEGTLLDRVKSREKFIEEQYERFHDYLIHVQL
                     ADFKKAFIELDDDEDNDKPDLYKEIIKRFHVDRLTWKDLFNDFEMHFYRYVFPYYDTL
                     YTLEKLSQKGFQIGVIANGKSKIKQFRLHSLGLMHVINYLSTSETVGFRKPHPKIFED
                     MIDQLGVLPEQIMYVGDDALNDVAPARAMGMVSVWYKQEDAEIEPLEEEVDFTITTVE
                     ELLTILPIKNDNKGENYGSIY"
     gene            2933..3733
                     /locus_tag="PPY92_002212"
     CDS             2933..3733
                     /locus_tag="PPY92_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDJ2626194.1"
                     /translation="MDLFTRKDGTSIHYSTLGEGYPIVLIHTVLDNYSVFNKLAAQLA
                     KSFQVVLIDLRGHGYSDKPRHIEIKDFSDDIVELLKYLYIEEVAFVCHEMGGIIGADI
                     SVRYPEFTSSLMLVNPTSIEGELPEERLFRKYAHIIRNWDPEKQDKFLNKRKYYRPRK
                     MNRFLKHVVDTNEISTKEEIQAVKEVFKNADISQTYRNVVVPTKIIAGEFGERTTRLE
                     AKEVADLIQNADFEVYQESSAFPFVEEQERFVEDIASFINKHHDEKHV"
     gene            3869..4375
                     /locus_tag="PPY92_002213"
     CDS             3869..4375
                     /locus_tag="PPY92_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626195.1"
                     /translation="MKKLLTASIIACSVVMGVGLVNTSAEAASGNSIDTVKQLIKGDQ
                     SLENVKIGESIKDVLTKYKNPMYSYNEDGTEHYYEFHTKKGMLLVTTDGKKNNGKVTH
                     ISMMYNDANGPTYQAVKNYVGKAVTHTEYSKVAGNFGYIEKGKTTYQFASAPKDKNIK
                     LYRIDLEK"
     gene            4537..5253
                     /locus_tag="PPY92_002214"
     CDS             4537..5253
                     /locus_tag="PPY92_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626196.1"
                     /translation="MRINDKILLENIEDYFNHKGLSPHLIDDIKEKVITDIKNSEKKD
                     QDYIEYKRKSPAQIILMIQRNLFALQMNPVIFFIINFILISYLYDKQYVQFQAITGMS
                     LFYCLVIFPMTIVVYLRVSQKNYLRSNKIEMIMGTIIAIISLLLIILQAFNITWGVIP
                     ITNFGHQFFFFIGIILVIAGIFYKRLEFSGIGLLFCQKTVDAMIHNPQSAQIFSLIIW
                     ILLVVLVIYFTIRLSSRTRL"
BASE COUNT         1944 a          670 c          937 g         1718 t
ORIGIN      
        1 agtatgtgga cttatataat aaatagaagc atataaagaa aaaaacagtt gtttgtttgt
       61 gcagcaactg cataaaagcc tctaatcact aaaggtgaag aggaacgcag ttggatgcta
      121 aggcacaact gcataaatcc ctctaatcgc taaagcgaaa agtgggatta aaaaggagat
      181 gtgataatgt gaagaaatcg ttaattgctt ttattttgat ttttatgctt gtcctgagtg
      241 gctgtggtat gaaagataat gataaacaag gtagcgatga taatggctcg tctaaatcgc
      301 cgtaccatag aattgtttcg ttaatgccta gtaatactga aattttatat gaattaggat
      361 taggtaaata catagttggt gtttcaacgg ttgatgatta tccaaaagat gtgaaagagg
      421 gtaagaaaca atttgatgct ttgaatctaa ataaagagga acttttaaag gcaaagccag
      481 atctaattct tgcgcatgag tcgcaaaagg caactgctaa taaagtattg tcatcattag
      541 agaaacaagg catcaaagta gtgtatgtta aagatgcaca atcaattgat gaaacataca
      601 acacatttaa gcaaattggg aaattaacgc atcatgataa gcaggctgaa caacttgttg
      661 aggaaactaa agataatatc gataaagtca tagattcaat tcctgctcat cataaaaaat
      721 caaaagtatt tattgaggtt tcatcaaagc ctgaaatata tacagcaggg aagcatacat
      781 tttttaatga tatgttagaa aaattagaag cccaaaatgt ttatagtgac attaatggtt
      841 ggaaccctgt aacgaaggaa agtattatta aaaagaaccc agatatatta atttcgacgg
      901 aagctaagac aagatcagat tatatggata tcatcaaaaa aaggggtgga ttcaataaaa
      961 ttaatgctgt caagaataca cgtattgaag ttgtaaatgg tgatgaagta tcaagaccag
     1021 gtccacgtat tgatgaagga ttaaaagaat taagagatgc aatttataga aaataaacca
     1081 ttctaattat gccccttatt gctacatgta aaaaaataca tgtttgagat aaggggtttt
     1141 taaaatatat ttagtgaatg atagcaacgc gagtatgtga ttgctataat gaatgtaatt
     1201 atcgatgaaa gaaaagagaa tgctatgaca tttaataaag tattattgag ctggatagtc
     1261 atattgatta taacaactag catatatcta ttttggcagt tgggggatat caatgatgta
     1321 tttaaccagt ctattttaat caatgttaga ttaccgagat tattagaagc attgttgaca
     1381 ggtatgatat taacagttgc aggtcttata tttcaaacag ttttaaataa tgcattggca
     1441 gatagcttta cattaggatt ggcaagcggt gctacatttg gttcaggatt agcattattt
     1501 ttaggtttaa caacgttatg gattcctgta ttttcaataa catttagttt gataacatta
     1561 ataactgtat tagtcattac gtcggtattg agccaaggct atccagttag aatcttaata
     1621 ttaagtggtt taatgattgg tgcattattc aattcacttc tatatttttt gattttatta
     1681 aaacctcgca aattaaatac aattgccaat tatttgtttg gtggctttgg tgatgcagaa
     1741 tactcaaatg tatccataat agcaatcaca tttatcattg cactgtttgg tatatttatc
     1801 attcttaatc aactaaagtt attgcaatta ggagaactaa aaagtcagtc actaggctta
     1861 aatgttcaat tgattacata tatcgcgtta tgtatagctt ctatgataac ggcgataaat
     1921 gtcgcatatg ttggcatcat tggattcatt ggtatggtga taccacaact cattagaaaa
     1981 tggcagtgga aacaatcatt aggaagacaa ttggctttga atattgtaat tggaggacaa
     2041 ataatggtta tggcagattt tattggtagc catatactgt caccagtaca aataccggca
     2101 agcattatta ttgcattaat tggtatacca gtgttatttt acatgctaat atctcagtcg
     2161 aaacggttac actagcacat gacatttgct aaaataaaaa taactataaa cataaagagg
     2221 gcataagcga tggatttgaa tcaaattaaa gcagttgtat ttgatttaga aggtacgttg
     2281 ttggacagag ttaaatctcg agagaaattt atcgaagagc aatatgaacg atttcatgac
     2341 tacttaattc atgttcaact ggcagatttt aaaaaagcat ttattgagct agatgacgat
     2401 gaagataatg ataaacctga tttatataaa gaaatcatta aacgtttcca tgtagatagg
     2461 ttaacttgga aagacttatt taatgatttt gaaatgcatt tttatcgtta tgtatttcct
     2521 tattacgata ctttgtatac actagaaaag ctatcgcaaa aaggctttca aattggtgtt
     2581 atcgcaaatg gtaaatctaa gattaaacaa tttcgattac attcacttgg tttgatgcat
     2641 gttattaatt atttatcaac atcagaaaca gttggttttc gtaaaccaca tcctaaaatt
     2701 tttgaagata tgattgatca actaggggta ttacctgagc aaattatgta tgttggcgat
     2761 gatgcgttaa atgatgtagc tccagcacga gctatgggca tggttagtgt atggtataaa
     2821 caagaagatg ctgaaattga accactcgaa gaagaagttg attttacaat tacaacagtg
     2881 gaagaattat taaccatttt accaataaaa aatgataata aaggagaaaa ttatggatct
     2941 atttactaga aaagatggaa catcgataca ttacagtaca ttaggtgaag gctatcctat
     3001 cgtattgatt catactgtac ttgataatta ttctgtgttt aataaattag cagcacaatt
     3061 agcaaaatca tttcaagttg tgttaattga tttacgtgga catggctatt ctgataaacc
     3121 tcgtcacatt gaaataaaag atttttctga tgacattgtt gaattactta aatatttata
     3181 cattgaagaa gttgcatttg tatgccatga aatgggtgga atcattggtg cggatatttc
     3241 agtacgttat cctgaattta catcatcact tatgttggta aatccaacat ctattgaagg
     3301 tgaattaccg gaagaacgtt tatttagaaa atatgcccat attattcgaa actgggatcc
     3361 tgaaaaacaa gataaatttt taaataagcg taagtattat cgtccgagaa aaatgaatcg
     3421 attccttaaa catgtcgtag atacaaatga aatatcaact aaagaagaaa ttcaagcagt
     3481 taaagaggta ttcaaaaacg ctgatatttc tcaaacttat agaaatgtcg tagtaccgac
     3541 aaaaattatt gcaggagaat tcggtgaaag aacaacaaga ttggaagcta aagaagtagc
     3601 tgatttaatc caaaatgcgg actttgaagt atatcaagaa tcaagtgcat tcccatttgt
     3661 tgaagagcaa gaaagattcg tcgaagatat agcttcattt atcaacaaac atcacgatga
     3721 aaagcatgtt taatttttag tgataagtga aatataaaag tagtaattat tatttgtaat
     3781 ataattgtaa tatgactgtt gttttagaaa tgattgttgt ataatggttg tgagcacaag
     3841 acgatataaa taaaaggaga gagtgcttat gaaaaagcta ctaacggcaa gtataattgc
     3901 atgttctgtt gtaatgggag taggcttagt gaacactagt gccgaagcag caagtggcaa
     3961 ctctattgat actgttaaac aattaattaa gggtgatcag tcattagaaa atgtgaaaat
     4021 tggcgaatct attaaagatg ttttaactaa gtacaaaaat cctatgtatt cttacaatga
     4081 agatggaact gaacattatt acgaattcca tactaaaaaa ggtatgttat tagtaactac
     4141 tgatggtaag aaaaacaatg gtaaagtaac tcatatttca atgatgtata atgatgctaa
     4201 tggtccaaca tatcaagctg ttaaaaatta tgttggcaaa gcagtaacac atacggaata
     4261 tagcaaagtt gctggtaatt ttggatatat tgaaaaaggc aaaacgactt atcaatttgc
     4321 ctcagcacca aaagataaaa acataaaatt atatcgtatt gatttggaaa aataataatt
     4381 aaattaatcg tgcttaaccg acaagttaaa tcgcaatact tgtcggttat tttattacat
     4441 ggcataatac agtgaatatt aatttttatg atttgcgaag gggaaattaa accaatacaa
     4501 taaataaaga tgtttcattt taaaggagta ctaccaatgc gtattaacga taaaatttta
     4561 ctagaaaata tagaagatta ctttaatcat aaaggtttat caccgcattt gattgatgat
     4621 attaaagaga aagtaattac tgatataaaa aattctgaaa agaaagatca agattatatt
     4681 gaatataaaa gaaaatctcc agcacaaatc atattaatga ttcaaaggaa tttatttgct
     4741 ttacaaatga atccagttat tttctttatt ataaacttca ttctcatatc ctatttatat
     4801 gataaacagt atgttcagtt tcaagctatt actggaatga gtctatttta ttgtttagtg
     4861 atttttccaa tgactattgt tgtataccta agggtgtcac aaaagaatta cttgcgtagt
     4921 aataaaatag aaatgattat gggtacaatt atcgctatca tatccttgtt attaattata
     4981 ttacaagcat ttaatattac ttggggcgtt ataccaatta caaattttgg acatcaattt
     5041 ttctttttca ttggtattat tttagtaatt gccggcatat tttataagcg acttgagttt
     5101 tcgggaatcg gcttattatt ttgtcaaaaa accgtcgatg caatgattca taatccacaa
     5161 tcagcccaga ttttttcatt aattatatgg atattattag tagttctagt tatatatttc
     5221 acaattagat tatcttcacg tacaagatta taaatatgat aaaactatt
//
LOCUS       DANBWH010000178         5259 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.184, whole genome shotgun sequence.
ACCESSION   DANBWH010000178 DANBWH010000000
VERSION     DANBWH010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5259
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.184"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            16..408
                     /locus_tag="PPY92_002215"
     CDS             16..408
                     /locus_tag="PPY92_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HDJ2626197.1"
                     /translation="MKKVINVVGAIIFSDNKILCAQRSEEMSLPLMWEFPGGKIEKNE
                     TEKEALIREIREEMKCDLIVGDKVITTEHEYDFGIVKLTTYKCTLNKELPTLTEHKSI
                     EWLSINELDKLNWAPADIPAVNKIMTEG"
     gene            410..3271
                     /locus_tag="PPY92_002216"
                     /pseudo
     CDS             410..3271
                     /locus_tag="PPY92_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
     gene            3578..4351
                     /locus_tag="PPY92_002217"
     CDS             3578..4351
                     /locus_tag="PPY92_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDJ2626198.1"
                     /translation="MIHSRKLRLWLYLVLLAVFIGACGMKKEESSKDKQIKENFNKTL
                     SLYPTKNLEDFYDKEGFRDEEFEKGDKGTWIIHSKMTIETNGKNMESRGLVLYVDRNT
                     RTTKGEFIVRELWEDKKGYSRSKEKEYPVKMEHNKIIPTKPIADDKLRKEIEDFKFFV
                     QYGDFKDINDYKDGDISYNPNVPSYSAKYQLSNNDYNVKQLRKRYDIPTKKAPKLLLK
                     GDGDLKGSSIGHKNLEFIFIENKEENIYFTDSINFKPTE"
     gene            4463..5236
                     /locus_tag="PPY92_002218"
     CDS             4463..5236
                     /locus_tag="PPY92_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDJ2626199.1"
                     /translation="MMHSKKLMLGICLVLLIILIVGYVIMTKINSRSAQIKDTFNQTL
                     KLYPTKNLEDFYDKEGFRDQEFEKGDKGNWIVDSEMVIELKDKKMESRSMVLYINRNT
                     RTTKGNFIVRELWEDSKGYAQSKDTKYPVKMEHNRIIPTKPIADDKLRKEIENFKFFV
                     QYGDFKDINDYKDGDISYNPNVPSYSAKYQLKNDDYNVKQLRKRYNIPTNKAPKLLLK
                     GDGDLKGSSIGSKNLEFTFVENKEENIYFSDSINFKPTE"
BASE COUNT         2136 a          559 c          934 g         1630 t
ORIGIN      
        1 tgaagggtga gtcttatgaa aaaagtaatc aatgtagtag gagctattat tttttctgat
       61 aacaaaattc tttgtgcaca gagaagtgaa gaaatgagtc tacctttaat gtgggaattt
      121 cctggcggta agattgaaaa gaatgaaact gaaaaagaag ctttgattag agaaattaga
      181 gaagaaatga aatgtgatct aattgttgga gacaaagtca taactacaga acatgaatat
      241 gattttggca ttgttaagtt aacaacatac aaatgtactt taaacaaaga gttaccaact
      301 ttaactgaac ataagagtat tgaatggttg tcaataaacg aacttgataa attaaattgg
      361 gccccagcgg atataccagc cgttaataaa attatgactg agggataata tgagtagatt
      421 actaaatgat ttcaatcaat ctttaaaaaa aggttttata gacaaagata tttctcataa
      481 agggaattat acaccgaagc ttttagtaaa taataaaaat gaaaaagtcc tttcaacaat
      541 tatagatgaa cttcaaaaat gtgaaacgtt ctatttttct gtagctttta taacagaaag
      601 cggattagcc agcttaaagg ctcaactatt agatttaagc aataagggtg ttaaagggaa
      661 aatattaaca tctaattact taggatttaa tagtcctaaa atgtatggag aattacttaa
      721 attaaaaaat gtagaggtaa gattaactga tattgctgga ttccatgcca aaggatatat
      781 ttttgaacat aaggattata gttctatggt aataggaagc tctaatttaa catctaatgc
      841 gttaaaggtt aattatgagc ataatgtttt actgtctact atgaaaaatg gtgatttagt
      901 tgatagtgta aaaaatgaat ttgaattgtt atggcaaaag agtaccccac tgactgagca
      961 atggattaat tcatataaag aatcatttga gtaccgttcc ttagaaaaat tagcggaggt
     1021 tgaacaaact caaatgctgc tagctgataa ggtgaaaaaa tcagtggaaa ttgtacccaa
     1081 tttaatgcaa gcagaagcat taaggtcatt aaaagctatt agggataaaa ccaaagataa
     1141 ggcgttaata atatctgcaa ctggtacagg taaaacgatt ttatgtgcat tagatgttag
     1201 agaagtaaac cctaataaat ttttatttat tgttcataat gaggggattt taaatagagc
     1261 taaggaagaa tttaaaaaag tattacctat aaaaaatgat agtgattttg gattgttgac
     1321 aggaaaacat agagatgtcg atgccaaata tttatttgca accattcaaa cattatctag
     1381 agatgataat tttaagcaat ttgatgaaaa tgaatttgat tatattgttt ttgatgaagc
     1441 acatcgttct gctgcatcta cttatcaacg agtatttaac tacttcaaac ctaagttcat
     1501 gctgggaatg actgctacac cagaaagatc agatgaatta agtatatttg aactgtttga
     1561 ttataatatt gcatatgaaa taaggttaca agcagcgtta gaaagtgata ttttatgtcc
     1621 atttcattat tttggtgtga ctgattatgt acatcaaggt attaaagaag atgatgtaac
     1681 taaactgaga tatttaactt ctgatgaaag agttaattat attattcaaa agacagatta
     1741 atatggatat tcaggtgaaa ttttacaagg attaattttt gtgagtagca aaaaagaagc
     1801 ttatgattta gcggataaac taagttcaaa agggattaaa agtgttgctt taacaggaga
     1861 tgatagtgtt aattatcgcc aaattgtaat agaaaaatta aaagaaggta agattaacta
     1921 tattataact gtagatcttt ttaacgaagg tattgatatt ccagaagtca atcaagttgt
     1981 aatgttgaga ccaacagagt ctagtattat attcattcag caacttggta gaggattaag
     2041 aaaaagtgca aataaagagt atgttactgt aattgatttt attggtaatt ataagaccaa
     2101 ttatttaatt ccgattgcgc tttctgggga tcaatcgcaa aataaagata attataagaa
     2161 gtttttaacg aataacgatt cgattaatgg agtatctaca attaattttg aagaagttgc
     2221 taaaaaacag atttacaatt cattggatgc agtatcattg aatcaaaata aattaatatt
     2281 aaaagcttat gaagaagttg aaaatagatt gggacacatg ccgttactaa tggattttat
     2341 acaacaacat tctatagatc caagcgttat attttctaaa tttagtaatt attacgaatt
     2401 tttattaagg tataaaaaaa tagatgcttt attgacggaa aatgaatcta aaaatctggt
     2461 tttcttttca agacaaatag cacctggatt gaaaagaatc gatagtttag ttttagaaga
     2521 acttttaaaa aatgaattaa catatgatga attaaaaaat aaaatgttga acgaagttaa
     2581 ggatataaca gaagatgata tagatacttc attaagaatt ttagattttt cattttacaa
     2641 cgcaggaatt gaaaaaatct atggaagtcc cattatagaa tgtaatgaaa gaatgataag
     2701 actaagcgat gcatttacta atgctctaag taatcaaaca tttaaaatat ttttagaaga
     2761 tcttattgaa ttatcaaagt ataacaatga aaaataccaa aaaggtaaaa atggactgat
     2821 tttatataat aaatattcaa gagaagattt tagcaaaata tttaattgga ataaaaatgg
     2881 ttcgtctgta atcatgggat acatgataag aagccaagaa atgccaatat ttatcactta
     2941 tgataagcat gaggatataa gtgatagtac aaaatatgaa gatgaatttt taagtcaaga
     3001 tgaattaaaa tggtttacta aatctaatag aacgctaaaa tcaaaagaag ttcagaaaat
     3061 tttgtcccat agagcaaaag gaattaaaat gtatattttt gtacagaaaa aagatgatga
     3121 tggtatatat ttttattatt taggaactgc tggatatata gaaggttcag agaagcaaga
     3181 taaaatgccg aatggttcaa acgtagtaac gatggattta gcactcgata aagctgtaag
     3241 agatgatata tatcgctata taacaaatta atgtaaaccg aaaaatgaat gttagtaaag
     3301 atgtatttaa acatcaattg taataagtaa agacattgaa gtttcagtgt tattatttta
     3361 aatataagcg caatttatct taattaatta gttggttagg ccaataaaaa ttataaagat
     3421 atttgcgctt tttaaataat ttgtgatgtt gatatgtgaa aaatacaact aacaattaaa
     3481 atccacgagg atttaatcat aaaatttcct ctagtattat taatattgga atttgaactt
     3541 aactttgcat caaaaataaa aatggggatg tgaaatgatg attcattcaa gaaagttgag
     3601 gctttggctg tatttagtat tattagctgt ttttataggc gcttgcggaa tgaaaaagga
     3661 agaaagcagt aaggataaac aaattaaaga aaacttcaac aaaacgttga gcttatatcc
     3721 aactaaaaat cttgaagact tttatgataa agaaggtttt cgagatgaag agtttgaaaa
     3781 aggagataaa gggacttgga taattcactc taaaatgaca atagaaacaa atggaaagaa
     3841 tatggaatcc agaggcttgg tcctgtatgt agaccgcaat accagaacga caaaaggtga
     3901 atttattgtg agagaattat gggaagataa aaaaggatac tcacgtagta aagaaaaaga
     3961 atatccagtg aaaatggaac ataataaaat tatcccaact aaaccgatag ctgatgataa
     4021 attaagaaaa gaaattgaag actttaagtt ttttgtacaa tacggtgact ttaaagatat
     4081 taatgattat aaagatggtg acatttcata taatcctaat gttccaagtt attccgcaaa
     4141 atatcaattg agtaacaatg actacaatgt gaagcaactt agaaagaggt atgatatacc
     4201 aactaagaaa gctccgaaac tattgttaaa aggtgatggt gatttaaaag gatcatctat
     4261 aggacacaaa aatttagagt ttatatttat agaaaataaa gaagaaaaca tctattttac
     4321 ggatagtatt aatttcaaac ctactgaata gggttaatag aagtttgaaa tgaaaatttc
     4381 ataaagtgtt caaaataatt tttcgcttta gttgtgtaaa ttaaagattg agtcagtatt
     4441 aaaaatataa ggtggagaaa tgatgatgca ttcaaagaaa ttaatgctcg gtatatgttt
     4501 ggtattactc attatattga ttgtaggtta tgtcattatg acaaaaataa atagtcgaag
     4561 cgcccaaatt aaagacacat ttaatcaaac attaaaatta tatccaacca aaaatcttga
     4621 agacttttat gataaggaag gctttcgaga tcaagagttt gaaaagggcg acaaaggcaa
     4681 ttggattgtt gattctgaga tggtaattga gctgaaggat aaaaaaatgg aatctagaag
     4741 tatggtgctc tatataaatc gtaatactag aacaacgaaa ggtaatttta ttgttagaga
     4801 attgtgggaa gatagtaaag ggtatgcaca aagtaaagat acaaaatatc cagtgaaaat
     4861 ggaacataat cgaattatcc caacgaaacc gatagctgat gataaattaa gaaaagaaat
     4921 agaaaacttt aaattctttg tacaatacgg tgactttaaa gatattaatg attataaaga
     4981 tggtgacatt tcatataatc ctaatgttcc aagttattcc gcaaaatatc aattgaaaaa
     5041 cgatgattac aatgtgaagc aacttcgaaa aagatataat attccaacca acaaagctcc
     5101 gaaactatta ttgaaaggtg atggtgattt aaaaggttca tcaataggat caaaaaatct
     5161 agaatttacc tttgtagaaa ataaagaaga gaacatttat ttttctgata gtattaattt
     5221 caaacctact gaatagggtt aatagaagtt tggaatgaa
//
LOCUS       DANBWH010000179         5122 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.186, whole genome shotgun sequence.
ACCESSION   DANBWH010000179 DANBWH010000000
VERSION     DANBWH010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5122
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.186"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            200..2014
                     /locus_tag="PPY92_002219"
     CDS             200..2014
                     /locus_tag="PPY92_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDJ2626200.1"
                     /translation="MNKTKGFTKYKKMRYMPGLDGLRAIAVLGIIIYHLNKQWLTGGF
                     LGVDTFFVISGYLITSLLLKEYDDTGIIKLKSFWIRRLKRLLPAVIVLLMVVGTATLL
                     LKSDNIIRVKHDIIAAIFYVSNWWYIAKDVNYFEQFSFMPLKHLWSLAIEEQFYIFFP
                     VILVTLLLTIKKRYKIGFIFWGVSIISLGLMMFIYSINGDHSRVYFGTDTRLQTLLLG
                     VILAFLWPPFKLKNDPPKVVKYVIDSIGSLSFIVLILLFFIINDETNWIYDGGFYLIS
                     ILTLFIIASVVHPSTWIAKIFSNPVLVFIGKRSYSLYLWHFAVISFVHSYYVDGQIPV
                     YVYFIDISLTIIFAELSYRFIETPFRKEGIKALNWRPSYIPQFIRMAIVVTLLIPFML
                     ILVGAFNKYGKDIIGEKANSFDTTIEDNYLMRIAPIDNIHIDGLVSEKKKESSDVYNN
                     IKPLLIGDSVMVDIGESFKSSVPKSRIDGKVGRQLYQTLPLVKANYSQYKKSSDQVVL
                     ELGTNGDFTVKQLDDLLNQFGKAKIYLVNTRVPRIYEANVNRLLADAAKRKSNVTLID
                     WNKRSQGHSEYFAPDGVHLEYKGVLALKDEILKALKKK"
     gene            2217..4826
                     /gene="clpB"
                     /locus_tag="PPY92_002220"
     CDS             2217..4826
                     /gene="clpB"
                     /locus_tag="PPY92_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDJ2626201.1"
                     /translation="MDINKMTYAVQSALQQAVELSQQHKLQNIEIEAILSAALNESES
                     LYKSILERANIEVDQLNKAYEDKLNTYASVEGDNIQYGQYISQQANQLITKAESYMKE
                     YEDEYISMEHILRSAMDIDQTTKHYINNKVEVIKEIIKKVRGGNHVTSQNPEVNYEAL
                     AKYGRDLVEEVRQGKMDPVIGRDEEIRNTIRILSRKTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVKKDVPESLLDKTVFELDLSALVAGAKYRGEFEERLKAVLKEVKESDGRIILFI
                     DEIHMLVGAGKTDGAMDAGNMLKPMLARGELHCIGATTLNEYREYIEKDSALERRFQK
                     VAVSEPDVEDTISILRGLKERYEVYHGVRIQDRALVAAAELSDRYITDRFLPDKAIDL
                     VDQACATIRTEMGSNPTELDQVNRRVMQLEIEESALKNESDNASKQRLQELQEELANE
                     KEKQAALQSRVESEKEKIANLQEKRAQLDESRQALEDAQTNNNLEKAAELQYGTIPQL
                     EKELRELEDNFQDEQGEDTDRMIREVVTDEEIGDIVSQWTGIPVSKLVETEREKLLHL
                     SDILHKRVVGQDKAVDLVSDAVVRARAGIKDPNRPIGSFLFLGPTGVGKTELAKSLAA
                     SLFDSEKHMIRIDMSEYMEKHAVSRLIGAPPGYIGHDEGGQLTEAVRRNPYSVILLDE
                     VEKAHTDVFNVLLQILDEGRLTDSKGRSVDFKNTIIIMTSNIGSQVLLENVKETGEIT
                     ESTEKAVMTNLNAYFKPEILNRMDDIVLFKPLSIDDMSMIVDKILTQLNIRLLEQRIS
                     IEVSDDAKAWLGQEAYEPQYGARPLKRFVQRQIETPLARMMIKEGFPEGTTIKVNLNS
                     DNNLTFNVEKIHE"
     gene            complement(4885..>5122)
                     /locus_tag="PPY92_002221"
     CDS             complement(4885..>5122)
                     /locus_tag="PPY92_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDJ2626202.1"
                     /translation="QFLSINDVHTSIKLIEMNQGISFLPIYITTDSDYNISVINTKIL
                     QAPISFTYIYSKKESPEILVFIKSFKKYIANEQL"
BASE COUNT         1895 a          656 c          958 g         1613 t
ORIGIN      
        1 cgagttgcat tacaaattgg atttcgaagt aagaatcatc attggtaatt tttttaattt
       61 aacagtattt tagtcaatat gattttgatg atgtcgtaat tcaataatat acatatgcat
      121 ttcaatattc ttttaaacag cataactaaa tttaattaag catctaagaa gaacagtttt
      181 aaaagggaag aatgcaaaaa tgaacaaaac aaagggtttt acaaagtata agaaaatgag
      241 atatatgcca gggctcgatg gtttgagggc aatcgctgtt ctaggaatta ttatttacca
      301 cttaaacaag caatggttga caggtggctt tttaggtgtg gatacatttt ttgtgatctc
      361 tggttattta attacaagct tattactcaa agagtatgat gacacaggta tcattaaatt
      421 gaaaagcttt tggatacgtc gtttaaaacg tttattacca gcagtcatag ttttattaat
      481 ggttgtaggg acagcaacct tattattaaa atcagataat atcattaggg ttaaacatga
      541 tattattgct gcgatatttt atgtatcaaa ctggtggtat atagcaaaag atgttaatta
      601 ttttgagcaa ttttcattta tgccattaaa gcatttatgg tctttagcaa ttgaagaaca
      661 gttttacata tttttcccag ttattttggt tacattattg ttaacaatta aaaagcgata
      721 caaaatagga tttatttttt ggggagtatc aataatttct ttagggttaa tgatgtttat
      781 ctacagtatt aatggggatc attcacgagt gtattttggt acagatacta gattacagac
      841 attgttactg ggtgttattt tagctttttt atggccaccg tttaaattga aaaatgatcc
      901 acctaaagtt gtaaaatatg ttattgatag cataggtagt ttatcattta tagtacttat
      961 attattattt ttcattatta atgatgagac gaattggata tatgatggtg gtttctattt
     1021 aatatccata ttaacgttat ttattattgc cagtgtcgtt catccatcta catggatagc
     1081 gaagatattt tcaaatccag tgttagtatt tatcgggaaa aggtcttata gtttatattt
     1141 atggcatttt gcagtaatta gtttcgtaca tagttactat gtagatggac agatacctgt
     1201 atatgtgtac tttatagata taagtttaac aattatattt gcagagctat catatcgctt
     1261 tatagaaact ccatttagaa aagaaggtat taaagcttta aattggcgac cttcttatat
     1321 accacaattt ataagaatgg caattgtagt aaccttgtta attccattta tgttgatttt
     1381 agtaggtgca ttcaataaat atggtaaaga cattattgga gaaaaagcga atagctttga
     1441 taccactatt gaagataatt atttaatgcg gatagcacca attgataaca ttcacattga
     1501 tggcttagta agtgagaaga aaaaggaatc ttccgacgta tataataata ttaaacctct
     1561 tttaatcggt gattcagtaa tggttgatat cggtgagtca tttaagtcat cagttcctaa
     1621 gtctagaatt gatggaaaag tagggcgtca attgtatcaa accttacctt tagttaaagc
     1681 gaattattca caatataaaa aatcatctga tcaagtcgta ttagaattag gtacaaatgg
     1741 cgactttact gtcaaacagc tcgacgattt acttaatcaa tttggaaaag ccaagattta
     1801 tttagttaat acacgtgttc caagaattta tgaggcaaat gtaaatcgat tattagctga
     1861 cgcggcgaaa cgaaagtcca atgtcacatt aattgattgg aataagcgat cacaaggaca
     1921 tagtgaatat tttgcaccag acggtgtaca tttagagtac aaaggagtcc tagctttaaa
     1981 agatgaaata ttaaaagcac ttaaaaagaa ataacatatg tttaataagt ctagttaatg
     2041 tgtaacgtaa cattagctag atttttttat tcaaaaaaat atttacaaat attaggaaat
     2101 ttaagtgtaa aagagttgat aaatgattat attgggacta taatataatt aaggtcaaag
     2161 aaagtcaaag tcaaaagtgc aacttgacca actctacaaa ttagaggtga aattatatgg
     2221 atataaataa aatgacatat gctgttcaaa gtgctttaca acaagcagtt gaactgagtc
     2281 agcaacataa attacaaaat atagaaattg aggcaatttt aagcgctgcc ttaaatgaaa
     2341 gtgaaagctt atataaaagt attttagaac gagcaaatat tgaggtagat caattaaaca
     2401 aagcttatga agacaaacta aacacgtatg catctgtaga aggtgacaat atacaatatg
     2461 gtcaatatat tagccaacaa gcaaaccaat tgataactaa ggctgaatca tacatgaaag
     2521 aatatgaaga tgaatatatt tcaatggagc atattttacg ttcggcaatg gacattgatc
     2581 aaacaacaaa acattatata aataataaag tagaagttat caaagaaatt attaaaaaag
     2641 taagaggggg aaatcacgtg acatcacaaa atccagaagt taattacgaa gcattagcta
     2701 aatatggccg cgacttagta gaagaagtta gacaaggtaa aatggatcct gttataggaa
     2761 gagatgaaga aattcgaaat acgattcgta ttttaagtcg taaaactaaa aacaaccctg
     2821 tgctcattgg tgaaccaggt gttggtaaaa ctgcaattgt tgaaggatta gcgcaacgta
     2881 tagttaagaa agatgtgcca gaatcattat tagataaaac tgtttttgag ttagatttaa
     2941 gcgcattagt agcgggcgct aaatatcgtg gtgaatttga agagagatta aaagcagtcc
     3001 taaaagaagt taaagagtct gatggtagaa ttatattatt tattgatgaa atccatatgc
     3061 ttgtaggtgc tggtaaaaca gatggtgcca tggatgcagg caacatgcta aaaccaatgt
     3121 tagcacgagg agagttacat tgtattggtg caacaacttt aaatgaatat cgagaatata
     3181 ttgaaaaaga ttcggcatta gagcgtcgtt tccaaaaagt agcagttagt gagcctgatg
     3241 ttgaagatac aatttcaatt ttacgtggtt taaaagaacg atatgaagtg tatcatggtg
     3301 tgcgtattca agatagagcc ttagttgctg ccgctgaatt gtctgatcgt tacatcactg
     3361 atcgtttttt accagataaa gcgattgatt tagttgacca agcatgtgca acaattcgta
     3421 cggaaatggg atcaaatcca actgaattgg atcaagttaa tagacgtgtc atgcaattag
     3481 aaattgaaga aagcgcactt aaaaatgaat ctgacaatgc gagcaaacag agattacaag
     3541 aactacaaga agagcttgcc aatgaaaaag agaaacaagc agcacttcaa tctcgtgtag
     3601 aatcagaaaa agaaaaaata gcaaatttac aagaaaaacg tgcgcaacta gatgaaagta
     3661 gacaagcgtt ggaagatgca caaacaaata acaatttaga aaaagctgct gaactacaat
     3721 atggaacaat tcctcaattg gaaaaagaac ttagagaatt agaggataat ttccaagatg
     3781 agcaaggtga agatacagat cgaatgattc gtgaagttgt aacagacgaa gaaattggcg
     3841 atattgtcag ccaatggaca ggcataccag tttcaaaatt agttgaaaca gaacgtgaaa
     3901 aattacttca cttaagtgac atcttgcata aacgtgttgt aggtcaagat aaagcggttg
     3961 acctggtttc agatgcagta gttagagcaa gagcaggtat taaagatcca aacagaccta
     4021 ttggtagttt cttattccta ggtccaactg gagtaggtaa aactgaatta gctaaatcat
     4081 tagctgcatc attatttgat tctgaaaaac atatgattcg tattgatatg agtgaatata
     4141 tggaaaaaca tgcagtatca agattgatag gggcacctcc aggatatatt ggacatgatg
     4201 aagggggtca attaactgaa gcggttcgtc gtaatccata ctcagttatc ttattagatg
     4261 aggttgaaaa agcgcatact gacgtcttta atgtattatt gcaaatttta gatgaaggcc
     4321 gtttaactga ttctaaagga cgtagcgttg attttaaaaa tactattatt attatgacaa
     4381 gtaatattgg atctcaagtt ttattagaaa acgtaaaaga gactggtgaa attacagaat
     4441 caacagaaaa agctgttatg acaaatttaa atgcatattt caaaccagaa attttgaatc
     4501 gtatggatga catcgtatta tttaaacctc tatctattga tgacatgagt atgattgtag
     4561 acaaaatatt aacgcaatta aatataagat tattagaaca gcgaatctca attgaagttt
     4621 ctgatgatgc taaagcttgg ctaggtcaag aagcttatga acctcaatac ggtgcaagac
     4681 cattaaaacg ttttgtacaa cgccaaattg aaacaccatt agcacgtatg atgattaaag
     4741 agggattccc agaaggtaca acgattaaag ttaatttaaa ttcagacaat aacttaacgt
     4801 ttaatgttga aaaaattcat gaataaaatt gaaccaagaa tgtgatgatt aatcgcattc
     4861 ttggtttttt agatttgaaa tttattataa ttgttcattg gcaatatact ttttaaatga
     4921 tttaataaaa accaatattt ctgggctttc ttttttgctg tatatatatg taaatgaaat
     4981 aggcgcttgt aaaattttcg tgttaataac cgaaatattg taatcactat ctgttgttat
     5041 ataaataggc aggaatgata taccttgatt catttcgatt aatttaattg aagtatgcac
     5101 atcattgata gatagaaatt gt
//
LOCUS       DANBWH010000180         5106 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.187, whole genome shotgun sequence.
ACCESSION   DANBWH010000180 DANBWH010000000
VERSION     DANBWH010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.187"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            41..1888
                     /gene="typA"
                     /locus_tag="PPY92_002222"
     CDS             41..1888
                     /gene="typA"
                     /locus_tag="PPY92_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDJ2626203.1"
                     /translation="MTNKREDVRNIAIIAHVDHGKTTLVDELLKQSGIFRENEHVDER
                     AMDSNDIERERGITILAKNTAVDYKGTRINILDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLKPVVVVNKIDKPSARPEGVVDEVLDLFIELEANDEQ
                     LEFPVVYASAVNGTASLDPEKQDDNLQSLYETIIDYVPAPIDNSDEPLQFQVALLDYN
                     DYVGRIGIGRVFRGKMRVGDNVSLIKLDGTVKNFRVTKIFGYFGLKRLEIEEAQAGDL
                     IAVSGMEDINVGETVTPHDHQEALPVLRIDEPTLEMTFKVNNSPFAGREGDFVTARQI
                     QERLNQQLETDVSLKVSNTDSPDTWVVAGRGELHLSILIENMRREGYELQVSKPQVII
                     KEIDGVMCEPFERVQCEVPQENAGAVIESLGARKGEMVDMTTTDNGLTRLIFNVPARG
                     MIGYTTEFMSMTRGYGIINHTFEEFRPRIKAQIGGRRNGALISMDQGSASTYAILGLE
                     DRGVNFMEPGTEVYEGMIVGEHNRENDLTVNITKTKHQTNVRSATKDQTQTMNRPRIL
                     TLEEALQFINDDELVEVTPESIRLRKKILNKNVREKEAKRIKQMMQENE"
     gene            complement(2028..2195)
                     /locus_tag="PPY92_002223"
     CDS             complement(2028..2195)
                     /locus_tag="PPY92_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaI family protein"
                     /protein_id="HDJ2626204.1"
                     /translation="MRQVQCIICDAKVFIDERTTESKRLKNNPIRTFMCDDCKSRLDT
                     PKQRAQHYPLD"
     gene            complement(2197..2679)
                     /locus_tag="PPY92_002224"
     CDS             complement(2197..2679)
                     /locus_tag="PPY92_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626205.1"
                     /translation="MIQIKGAVKFPITLDSTTWIFDDRKVSIEDLEKGVFDGSKPIDF
                     EDNHEWNRAILEGQTNPPTLNSEIKYKKRAMLEGSFVINMTPFFKHSEPHDSATTIRL
                     SNEHDHLDIPIDLLPYLFFQFAKDGKRLYSDNGVDSFIYNQEDGYSYRINHVTHIEVI
                     "
     gene            2820..3095
                     /locus_tag="PPY92_002225"
     CDS             2820..3095
                     /locus_tag="PPY92_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HDJ2626206.1"
                     /translation="MAKQATMKNAALKQLTKDADEILHLIKVQLDNLTLPSCPLYEEV
                     LDTQMFGLQKEVDFAVKLGLVDREDGKQIMLRLEKELSKLHEAFTLV"
     gene            3409..4635
                     /gene="ftsW"
                     /locus_tag="PPY92_002226"
     CDS             3409..4635
                     /gene="ftsW"
                     /locus_tag="PPY92_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HDJ2626207.1"
                     /translation="MKNFRSILRYIGKTSKFIDYPLLVTYIVLSLIGLVMVYSASMVP
                     ATKGTLTGGIDVPGTYFYNRQLAYVIMSFIIVFFIAFLMNVKLLSNIKVQKGMIITIV
                     SLLLLTLVIGKDINGSKSWINLGFMNLQASELLKIAIILYIPFMISKKMPRVLSKPKL
                     ILSPIVLALGCTFLVFLQKDVGQTLLILIILVAIIFYSGIGVNKVLRFGIPAVLGFLV
                     VFVIALMAGWLPSYLTARFSTLTDPFQFESGTGYHISNSLLAIGNGGVFGKGLGNSAM
                     KLGYLPEPHTDFIFAIICEELGLIGGLLVITLEFFIVYRAFQFANKTSSYFYKLVCVG
                     IATYFGSQTFVNIGGISATIPLTGVPLPFISFGGSSMISLSIAMGLLLIVGKQIKVDQ
                     QRKKQQQKVDIRRQFN"
BASE COUNT         1756 a          703 c          933 g         1714 t
ORIGIN      
        1 aaaagtggga ttttacttaa gaaagaagga aactatttat atgactaata aaagagaaga
       61 tgtccgcaat atagcaatta ttgctcacgt tgaccatggt aaaacaactt tagtagatga
      121 gttgttaaaa caatctggta tattcagaga aaatgaacat gtcgatgaac gtgcaatgga
      181 ctctaacgat atcgaaagag agcgtggaat tacgattcta gccaaaaata cggctgttga
      241 ttataaaggt acacgtatta atattttgga tacaccagga catgcagact ttggtggaga
      301 agtagaacgt attatgaaaa tggttgatgg ggttgtctta gtagtagatg cgtatgaagg
      361 tacaatgcct caaacacgtt ttgtacttaa aaaagcgcta gaacaaaacc tgaaacctgt
      421 tgttgttgtt aataaaattg ataaaccatc agcacgtcca gagggtgttg tagatgaagt
      481 tttagattta tttattgaat tagaagcaaa cgatgaacaa ttagaattcc ctgttgttta
      541 tgcttcagca gtaaatggaa cagctagctt agatcctgaa aaacaagatg ataatttaca
      601 atcattatat gaaacaatta ttgattatgt accagctcca attgataaca gtgatgagcc
      661 attacaattc caagtagcat tgttggacta caatgattat gttggacgta ttggtattgg
      721 tcgtgtattc agaggtaaaa tgcgtgtcgg agataatgta tcactaatta aattagacgg
      781 tacagtgaaa aacttccgtg taactaaaat ctttggttac tttggattaa aacgtttaga
      841 aattgaagaa gcacaagctg gagatttaat tgctgtttca ggtatggaag acattaatgt
      901 tggtgaaact gtaacaccac atgaccatca agaagcattg ccagttctac gtattgatga
      961 gcctactctt gaaatgacat ttaaagttaa caattctcca tttgctggcc gtgaaggtga
     1021 ctttgtaaca gcacgtcaaa ttcaagaacg tttaaatcaa caattagaaa cagatgtatc
     1081 tttgaaagtt tctaacacag attctccaga tacatgggta gttgctggtc gcggtgaatt
     1141 gcatttatca atccttattg aaaatatgcg tcgtgaaggt tatgaattac aagtttcaaa
     1201 accacaagta attattaaag aaatagatgg tgtaatgtgt gaaccatttg aacgtgtgca
     1261 atgtgaagtg ccacaagaaa atgcaggtgc tgttattgaa tcattaggtg cacgtaaagg
     1321 tgaaatggtt gatatgacta caactgataa tggacttaca cgtttaatct ttaatgtacc
     1381 ggctcgtggt atgattggtt atacgactga atttatgtca atgacaagag gttacggtat
     1441 tattaaccat acatttgaag aatttagacc acgtattaaa gcacaaattg gcggtcgtcg
     1501 taatggtgca ttaatttcaa tggatcaagg ttctgcaagt acttatgcca ttttgggact
     1561 tgaagataga ggtgtaaact tcatggaacc tggtactgaa gtttatgaag gtatgattgt
     1621 tggtgaacat aatcgtgaaa atgatttaac tgttaacatc actaaaacaa aacatcaaac
     1681 taacgtacgt tctgcaacga aagaccaaac acaaacaatg aatagaccgc gtattctaac
     1741 attggaagaa gcgttacaat tcattaatga tgatgaactt gttgaggtta caccagaaag
     1801 tatacgttta agaaagaaaa ttttaaacaa aaatgttcgt gaaaaagaag caaagcgtat
     1861 caaacaaatg atgcaagaaa acgaataatt taacataata aaagagaagc tgagcgatga
     1921 aagttgctta gcttctcttt tggcatataa tatttgaaaa agtgtttaaa tgctattatt
     1981 tcctgttact ttcacatttt caaatttcta acttgtttat tcagatatta atcgagcgga
     2041 tagtgttgcg cacgttgttt aggtgtgtct aatcgacttt tacaatcatc acacataaat
     2101 gttcgaatag gattgttttt aagtcgttta gactcagttg tgcgctcatc tataaatact
     2161 tttgcatcac agataatgca ttgcacttgt ctcaaattaa atcacctcaa tatgagttac
     2221 atggttgatt cgatatgaat aaccatcttc ttgattgtaa ataaaactat ctacaccatt
     2281 atcgctgtaa agtcgtttac cgtcttttgc aaattggaaa aataaatagg gtaatagatc
     2341 tatcggtata tctaaatgat catgctcatt tgataatcga atagtagttg cagagtcatg
     2401 tggttcggaa tgtttgaaaa atggtgtcat attaatgaca aatgaacctt ctaacatggc
     2461 acgtttttta tattttattt ctgaatttaa agtaggcgga ttagtttgtc cttctaggat
     2521 agcacgattc cattcatgat tatcttcaaa gtcgattggt tttgaaccat caaatacacc
     2581 tttttctaaa tcttcgatgc taacttttct atcatcgaaa atccaagtcg tactatctaa
     2641 tgttatagga aactttacgg ctcctttaat ttgtatcatt ttcccactcc ctatcaatgt
     2701 atatagcatt attttaacac aaattgctaa cgacacattt taaatctact tgctttttaa
     2761 tatttagtaa gataaacttt tagtaagact tgagaattta tatagagggg gagcgtgtca
     2821 tggcgaaaca agcaacaatg aaaaatgcag ctttgaaaca attgactaaa gatgctgatg
     2881 aaatcttgca tctgattaaa gttcaactag ataatttaac attaccttca tgcccattat
     2941 atgaagaagt actagataca caaatgtttg gacttcaaaa agaagttgat tttgctgtta
     3001 aattaggttt agttgaccgc gaagatggca aacaaattat gttacgtctt gagaaagaac
     3061 tttcaaaatt acatgaagct tttacacttg tttaaggttt aatatataag catattaaaa
     3121 tggcgtgaga tatcgacaat tgagctgcgc cgattttata tttatagttt aattactgat
     3181 taaagtggct gttatgttgt cttcttgatg acatgtcagt cacttttatt gggttttagc
     3241 aattcacgat attaagtaaa caaatattct ataaattacc tcaagagacc tgttaatatt
     3301 tgtgattatg actgtgtcat gtatataata agtaacatgg atttttaaaa ttaaagtata
     3361 aaacgaaatc atttgaagta taaattgtag aattggatga ctgaatatat gaagaatttt
     3421 agaagtattt tacggtatat tggtaaaacc tcaaagttta ttgattatcc gttattagtt
     3481 acatatattg tattgagttt aattggatta gtgatggtat atagtgcaag tatggttcca
     3541 gcaactaaag gcacattgac tggtggtatc gatgttccag gaacgtattt ttacaaccga
     3601 caattagcat atgtcataat gagttttata attgtatttt ttattgcatt tttaatgaat
     3661 gttaaattac tgagtaatat taaagtgcaa aaaggtatga ttataactat cgtctcacta
     3721 ttattactga cgttagtaat aggtaaagat attaatggtt ctaaaagttg gataaactta
     3781 ggatttatga atttacaggc atctgagtta ttaaaaattg caatcatatt atatatccca
     3841 tttatgatca gtaaaaaaat gcctagagta ttaagtaaac caaaattaat tttaagtcct
     3901 attgtattag cattaggttg tacgttttta gttttcttac aaaaagacgt agggcaaaca
     3961 ttactaatat taattatttt agttgcgatt attttttatt caggaattgg ggtaaacaaa
     4021 gtcctaagat ttggtatacc agcagtgcta ggattcttag tagtatttgt cattgcatta
     4081 atggctggtt ggttaccaag ctatttaact gccagattta gtacgctaac agatccattc
     4141 caattcgaat caggaactgg ataccatatt tccaattcat tgcttgcgat aggtaacggt
     4201 ggcgtatttg gaaaaggatt aggaaatagt gcaatgaaat tgggctattt accagaacca
     4261 catacagatt ttatttttgc aattatttgc gaagaattag gtttaatcgg aggattgcta
     4321 gttattactt tagagttctt tattgtatat cgtgccttcc agtttgcaaa taaaacatca
     4381 tcatattttt ataaacttgt gtgtgttggg attgccacat actttggaag tcaaacgttt
     4441 gtaaacattg gcggtatttc ggcaacaatt ccattaactg gtgtgccatt gccatttatc
     4501 agctttggtg gatcatcaat gattagttta agtattgcta tgggattact tctgattgta
     4561 ggtaaacaaa tcaaagtaga ccagcaacga aagaaacaac aacaaaaagt tgatataaga
     4621 agacaattta attaaaaaaa tactagccaa tatttagtac gagatgactt ctgtcattaa
     4681 gcaatgctaa acattggcta tttatatact ctttgtcaaa ttggactgat gagttcaata
     4741 acggaagtta ggcaaccaaa caacgcttaa cttccttttt gttttgatat tacgagatta
     4801 ctcatgatta tcaaatttta taaacaaaac aacgtataat tataactaaa ttttatgact
     4861 aaaaattact aaatgtggtg gcgaaattag aatcatgatg ataagataaa gttatcgcta
     4921 ttgataataa atgttgatgt tttgcaattt tatttttaat tttttagagt tgtagaaatc
     4981 gaaaatgttt cacaacaaaa acgtgaacta cctgttgttg catagtcatt tttgaaatta
     5041 ttatttctga agcggaggtt ttaattgtct caagaatgtt aaattttgaa aatttgaaac
     5101 ataact
//
LOCUS       DANBWH010000181         5102 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.188, whole genome shotgun sequence.
ACCESSION   DANBWH010000181 DANBWH010000000
VERSION     DANBWH010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5102
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.188"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            419..784
                     /gene="crcB"
                     /locus_tag="PPY92_002227"
     CDS             419..784
                     /gene="crcB"
                     /locus_tag="PPY92_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HDJ2626208.1"
                     /translation="MQYVYIFIGGALGALLRYLISFLNTDGGFPIGTLIANLTGAFVM
                     GLLTALTIAFFSNHPTLKKAITTGFLGALTTFSTFQLELIHMFDHQQFITLLLYAVTS
                     YVFGILLCYVGIKLGGGLS"
     gene            781..1134
                     /locus_tag="PPY92_002228"
     CDS             781..1134
                     /locus_tag="PPY92_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HDJ2626209.1"
                     /translation="MISIILVMIGGGFGAIARSAITDYFNHKFTSKLPIATLIVNLVG
                     SFLIGLTIGLSISISWFPAFFVTGFLGGLTTFSTLAKELTLMMTPKFDINLFLNYSLL
                     QFIIGFIACYIGYHI"
     gene            1533..3068
                     /locus_tag="PPY92_002229"
                     /pseudo
     CDS             1533..3068
                     /locus_tag="PPY92_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(3131..3964)
                     /locus_tag="PPY92_002230"
     CDS             complement(3131..3964)
                     /locus_tag="PPY92_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDJ2626210.1"
                     /translation="MEVKTFYNGNTMPQIGLGTFRVENDENCMESVKYAIEQGYRSID
                     TAKVYGNEEQVGAGIRAGLESTGIAREDLFITSKLYFEDFGRENVAAAYEASLSRLGL
                     KYLDLYLVHWPGTNEAVMVDTWKGMEDLYKNNKAKNIGVSNFEPEHLEALLAQVSIKP
                     VINQVEYHPYLTQHKLKLYLAAQHIVMESWSPLMNAQILNDETIKDIAQELGKSPAQV
                     VLRWNVQHGVVIIPKSVTPNRISENFQIFDFELSDEQMTRIDGLNQDKRIGPDPKTFE
                     G"
     gene            complement(4176..5084)
                     /locus_tag="PPY92_002231"
     CDS             complement(4176..5084)
                     /locus_tag="PPY92_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HDJ2626211.1"
                     /translation="MDLSSPVVIGLIIAVVIALIFFVLFLVALGSKKKVKRQTEEKYE
                     QQEQNIKKSHEEALEKERIQNKKTITKQQEDYNHMVSTKDREIDALKLFSKNHSEYVT
                     DMRLIGIRERLVKEKRIRPEDMHIMANIFLPKDGFNNIERISHLVLTRTGLYIIDSQL
                     LKGHVYNGISGGQFKDLPPMEQVFDTLDLDKSRPQTIVMDQNDDKRSLSFVNYSDQIE
                     AIKQLAEDLQKELGAKYTPTSILYFNPKNEGDVTISNYNQNSAVKVLVGAEQLDEFFN
                     KFVFHGRIQYNVEDLQQMMDKIESFN"
BASE COUNT         1722 a          862 c          730 g         1788 t
ORIGIN      
        1 aattgtacta attttgtcta taatttttgc agatcgttca attaaatcac taaagcctga
       61 tgcagattgg attagaattg cgagtaaaag tttgccttgg attattctaa ttgttatttt
      121 agtacatatc ttttcaattg ttcgtacatt cggttttatt taaaaaagat aaaatgtcaa
      181 tgtagcgtta atacaaaaca atacaatgta tcatgctata atgagtaaaa caatttgatg
      241 acgttgttgc gtataaaaaa tattagattt tcgaaatcat aactatgcat ctaatcgcta
      301 tagttataca acaagatata acatataatg aggtttgata atgcatcgac aatttttgtc
      361 gtcgtgttgc caaaacctct tttttaaatt caaactactt cttttcgagg tgaaccaaat
      421 gcaatatgta tatattttta tcggtggtgc tttaggcgct ttattacgtt acctcatttc
      481 ttttctgaat actgacggag gttttccaat cggaacactg atagccaatt tgactggtgc
      541 ctttgtaatg ggattgctaa cagccttaac aattgcattt ttttcaaacc atccgaccct
      601 aaaaaaagct attacgactg gttttcttgg tgctttaacg actttttcaa catttcaatt
      661 agaattaata catatgtttg atcatcaaca atttataact ttactactat atgctgtaac
      721 aagttatgtc tttggtattt tgttatgtta cgtcggtata aaactaggtg gtggtttatc
      781 atgatatcaa tcattttagt catgattggc ggcggttttg gcgcaattgc tagaagtgcc
      841 attactgatt attttaatca taaatttact tcaaagttac ctatcgcaac attgatagta
      901 aatctagttg gtagtttttt aattggatta actataggcc tatcaatttc aatctcatgg
      961 ttccctgcgt tctttgtcac cggtttttta ggtggcttaa caactttctc aacgttagcc
     1021 aaggaactta cattaatgat gacaccaaaa tttgatatta acctttttct caattattca
     1081 cttttacaat tcatcattgg atttatagct tgttatattg gctatcatat ttaaaaataa
     1141 aatgcttcat tcagcaaata ggtaaattac gacaccttcc tgaacgaagc attttttaat
     1201 tttcatgcaa attttttaac accatataat gcctatcaaa ataaaacaat ctttgttgcc
     1261 gtttaaataa tgtgaatgtc aataaattct ccaaactagt cgaaaataaa acaggctggg
     1321 acataaatcc ctaaaaaaac agcagtaaga taattttcaa ttagaaaata tcttactgct
     1381 gttctctatt tatacaatac ttcgtattga atggcttcgc tttcctaggg tgccgtctca
     1441 gcctcggtct tcgactggca ctgctccctc aggagtctcg ccattaatac tacgtattaa
     1501 catgtaattt tacttttaca tactttaaaa aaataagaca ctttgcccaa cttacactac
     1561 caatagaaac ttctgttaga attccttaaa atgatatttc gcgatatgta aatgaaattg
     1621 ttgaaacgat acctgatagc gaattcgatg aattcagaca tcatcgtggc gcaacatcct
     1681 atcatccaaa aatgatgtta aaaatcatct tatatgcata tactcaatct gtattttctg
     1741 gtcgtagaat agagaaatta cttcatgaca gtattcgaat gatgtggtta gctcaagatc
     1801 aaacaccttc ttataaaact attaatcgtt ttagagtgaa tcctaatact gatgcgttaa
     1861 ttgaatcttt atttattcag ttccatagtc aatgtttaaa gcaaaatctt attgataata
     1921 attcaatttt tattgatggt acataagtag aagctaatgc caatagatat acatttgtgt
     1981 ggaagaaaag tattcaaaat cacgagaaga aatagattta attggtagtc atttagataa
     2041 agaaatcgaa gatttaaatc attctattta gaacgaagat tgtgctcaaa ttagaaaaca
     2101 gacccgtaaa aaagaactga gattaagaag ttcaaaaaga aatttgatga ttattccgaa
     2161 agaaaaaata aatatgaaga acaaaaatcg attcttaaag atagaaatag cttttctaaa
     2221 actgatcatg atgcaacttt tatgagaatg aaggaagacc atatgaaaaa tggccaactt
     2281 aagccaggat acaatttaca aatagcgaca aattctcaat ttttttatcc tatgacctat
     2341 ttcaaacccc aacagatact agaactttaa ttccattttt aacaatgatt caaaatacct
     2401 tcggttattt accagagtat attgtagctg atgcaggtta tgatagtgag caaaactata
     2461 tggctattat agatgatttt aataaaacgc cacttattac gtatggcatg tttattaaag
     2521 ataaaactag aaagtttaaa agtgacattt ttaacactca aaattggaaa tatgacgaac
     2581 ttaatgatga atttatatgt cctaataaca aaagaatagg ttttaaaaga tatgcatacc
     2641 gtaatgatag atatggtttt aaacgtgact tcaagctata tgaatgcgat gactgttcag
     2701 catgttcttt gaggcaacaa tgcatgaaac caaattcgaa atccaataag aaaatcatga
     2761 agaattataa ttgggaatac tttaaagccc aaattaatca aaagctttct gaaccagaaa
     2821 cgaaaaaaat ctatagtcaa agaaaaattg atgtagagct tgtttttgga tttatgaagg
     2881 ctattttggg tttcactcga atgtcagttc gaggaataaa taatgttaaa cgagagctag
     2941 gttttgtatt aatggcactt aatataagga aaatagcagc tcaacgagct gtacattata
     3001 aaatacatat caaaaaagct gatttctatc aaataattaa tagaaatcaa cttttttaca
     3061 ttgcctaaga acttaatgtc ccaagccctg acaattcatc taaccttttc atcagttgag
     3121 tgattttaat ctatccttca aaagtttttg gatcaggtcc aattctctta tcttgattta
     3181 aaccatcaat tcgcgtcatt tgttcatctg ataattcgaa atcaaatatt tggaagtttt
     3241 cagagattct gtttggtgtt accgatttag ggattataac cacaccatgc tgcacattcc
     3301 atcttaaaac aacttgggca ggtgactttc ctaattcttg agcaatgtct ttaattgtct
     3361 catcatttaa aatttgtgca ttcatcaatg gtgaccaaga ttccatcacg atatgttgtg
     3421 ctgccaaata taatttcaat ttatgttgcg ttaaatatgg atgatattca acttgattaa
     3481 ttacaggttt aattgacact tgtgccaaca aagcttccaa atgttcaggt tcaaaattgc
     3541 tgacacctat atttttagct ttattatttt tatataaatc ttccatacct ttccatgtat
     3601 caaccattac ggcttcgttc gtgcctggcc aatgtactag atacaaatct aagtatttta
     3661 aacctaatct agataaacta gcttcgtaag cagctgctac attttcacga ccgaaatcct
     3721 caaaatataa ttttgaagta ataaataagt cttctctagc aataccagtt gactccaatc
     3781 cggcacgaat gccagcacct acttgttctt cattcccata aacttttgcg gtatcaatac
     3841 tacgatatcc ttgttcaatg gcatacttaa cactttccat gcaattttca tcattttcca
     3901 cacgaaatgt ccctaaacca atttgtggca tcgtgtttcc attataaaat gttttaacct
     3961 ccataaatat cgcctcacct ttttgatgta ttataccctg ttatcataac aaatctgagt
     4021 tgaatacatg agaaaaaaca cttagagcaa tcaaccacta aaattctagt aatatctctc
     4081 aaacattaat caaattgtaa aagcaattat gtttaattta tgacaaacta aaaaagccga
     4141 agtcacaaca tatagtcatc acttcagcct aacatttaat tgaatgattc aattttatcc
     4201 atcatttgtt gtaagtcttc cacgttgtat tgaatacgac catggaatac aaatttgtta
     4261 aagaactcgt ctaattgttc agcaccgaca agcactttga cagcactatt ttgattataa
     4321 tttgaaatcg ttacatcgcc ttcattttta ggattaaagt ataaaattga agttggcgta
     4381 tatttggcac ctaattcttt ttgtaagtct tcagccaatt gtttaatcgc ctcaatttga
     4441 tctgaataat ttacaaaaga taatgaacgt ttgtcatcat tttgatccat cacaatagtt
     4501 tgtggtctag atttatctaa atccaatgta tcaaatactt gttccattgg tggtaaatct
     4561 ttaaattgac cgccactaat accattataa acatgacctt ttaacaattg agaatcaata
     4621 atataaagac cagttcttgt taatactaaa tgactaattc gttcaatatt attaaagcca
     4681 tcctttggta aaaagatatt tgccataatg tgcatatctt ctggtcgaat tcgtttttct
     4741 ttaactaatc tttcacgaat accaattaat ctcatgtccg ttacatattc actatgattt
     4801 ttcgagaaca attttaatgc gtcaatctca cgatcttttg tactaaccat gtgattataa
     4861 tcttcttgtt gttttgtaat tgtcttttta ttttgaatac gctctttctc taaagcttct
     4921 tcatgagact ttttaatgtt ttgttcttgt tgttcatact tttcttctgt ttgtcgctta
     4981 acttttttct tactacctaa ggcaactaag aaaaggacaa aaaagattaa tgcaatgact
     5041 actgcaataa tgagtccaat gactaccggt gaagataaat ccatcacaac aacgctcctt
     5101 tt
//
LOCUS       DANBWH010000182         5082 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.189, whole genome shotgun sequence.
ACCESSION   DANBWH010000182 DANBWH010000000
VERSION     DANBWH010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5082
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.189"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            4..801
                     /locus_tag="PPY92_002232"
     CDS             4..801
                     /locus_tag="PPY92_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDJ2626212.1"
                     /translation="MNRLHGQQVKIGYGDNTIINKLDVEIPDGKVTSIIGPNGCGKST
                     LLKALSRLLAVKEGEVFLDGENIHTQSTKEIAKKIAILPQSPEVADGLTVGELVSYGR
                     FPHQKGFGRLTAEDKKEIDWAMEVTGTDTFRHRSINDLSGGQRQRVWIAMALAQRTDI
                     IFLDEPTTYLDICHQLEILELVQKLNQEQGCTIVMVLHDINQAIRFSDHLIAMKEGDI
                     IATGSTEDVLTQEILEKVFNIDVVLSKDPKTGKPLLVTYDLCRRAYS"
     gene            837..1841
                     /locus_tag="PPY92_002233"
     CDS             837..1841
                     /locus_tag="PPY92_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDJ2626213.1"
                     /translation="MTNRENPTPLKFLSYIIGLSMILLITLFISTLIGDAKIQASTII
                     EAIFNYNPSNQQQNIINEIRIPRNIAAVIVGMALAVSGAIIQGVTRNGLADPALIGLN
                     SGASFALALTYAVLPNTSFLILMFAGFLGAILGGAIVLMIGRSRRDGFNPMRIILAGA
                     AVSAMLTALSQGIALAFRLNQTVTFWTAGGVSGTTWSHLKWAIPLIGIALFIILTISK
                     QLTILNLGESLAKGLGQNVTMIRGICLIIAMILAGIAVAIAGQVAFVGLMVPHIARFL
                     IGTDYAKILPLTALLGGILVLVADVIARYLGEAPVGAIISFIGVPYFLYLVKKGGRSI
                     "
     gene            1838..2854
                     /locus_tag="PPY92_002234"
     CDS             1838..2854
                     /locus_tag="PPY92_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDJ2626214.1"
                     /translation="MISSNNKRRQLIALAVFSILLFLGCTWSITSGEYNIPVERFFKT
                     LIGQGDAIDELILLDFRLPRMMITILAGAALSISGAIVQSVTKNPIAEPGILGINAGG
                     GFAIALFIAIGKINADNFVYVLPLISILGGITTALIIFIFSFNKNEGVTPASMVLIGV
                     GLQTALYGGSITIMSKFDDKQSDFIAAWFAGNIWGDEWPFVIAFLPWVLIIIPYLLFK
                     SNTLNIIHTGDNIARGLGVRLSRERLILFFIAVMLSSAAVAVAGSISFIGLMGPHIAK
                     RIVGPRHQLFLPIAILVGACLLVIADTIGKIVLQPGGVPAGIVVAIIGAPYFLYLMYK
                     TKNV"
     gene            3088..4056
                     /gene="dhaK"
                     /locus_tag="PPY92_002235"
     CDS             3088..4056
                     /gene="dhaK"
                     /locus_tag="PPY92_002235"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDJ2626215.1"
                     /translation="MMKKLINKKETFLTDMLEGLLIAHPELDLIANTVIVKKAKKEHG
                     VAIVSGGGSGHEPAHAGFVAEGMLDAAVCGEVFTSPTPDKILEAIKAVDTGDGVLLVV
                     KNYAGDVMNFEMAQELAEMEGINVQTVIVRDDIAVTNEVQRRGVAGTVFVHKLAGYLA
                     EKGYSLTEIKSRVEALLPEIKSIGMAIEPPLVPTTGKYGFDIEDDKMEIGIGIHGEKG
                     IHREEVKDIDHIVGTLLDELYKEVTANDVILMVNGMGGTPLSELNIVTKYIQQNLAAR
                     TVNVAKWFVGDYMTSLDMQGFSITIVPNKPEYLEAFLAPTTSQYFK"
     gene            4098..4682
                     /gene="dhaL"
                     /locus_tag="PPY92_002236"
     CDS             4098..4682
                     /gene="dhaL"
                     /locus_tag="PPY92_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HDJ2626216.1"
                     /translation="MKVNDMKARLLNLEETFKKHESELTELDRAIGDGDHGVNMVRGF
                     SSLKDKLDDSSMQSLFKSTGMALMSNVGGASGPLYGFSFVKMSAVTKDDMDNQDFITL
                     IQAFAEAVESRGKVTLNEKTMYDVVARAAEKLENGETLTFNDLQQLADNTKDMVATKG
                     RAAYFGEESKGYIDPGAQSMVYILNALIGDEDNA"
     gene            4675..5037
                     /locus_tag="PPY92_002237"
     CDS             4675..5037
                     /locus_tag="PPY92_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDJ2626217.1"
                     /translation="MPKIILVSHSKEIASGTKSLLKQMAGDVDIIPIGGLPDGSIGTS
                     FDIIQEVLTKLEDDALCFYDIGSSEMNVDMAIEMYDGNHRVLKVDAPIVEGSFIAAVK
                     LSIGGSIDDALAEIKQSF"
BASE COUNT         1669 a          723 c         1050 g         1640 t
ORIGIN      
        1 attatgaatc gtttgcatgg acaacaagtt aaaattggtt acggggataa cacgattata
       61 aataaattag atgttgaaat accagatggc aaagtgacgt caatcattgg tcctaacggc
      121 tgcgggaaat ctactttgct aaaggcattg tcacgtttat tggcagttaa agaaggcgaa
      181 gtatttttag atggtgaaaa tattcataca caatctacga aagagattgc aaaaaaaata
      241 gccattttac ctcaatcacc tgaagtagca gatggcttaa ctgttgggga attagtttca
      301 tatggtcgtt ttccacatca aaaaggattt ggtagattaa ctgctgagga taagaaagaa
      361 attgattggg caatggaagt tacaggaact gatacattcc gacaccgttc aatcaatgat
      421 ttaagtggtg gtcaaagaca acgtgtttgg attgcaatgg cattagcaca aagaactgat
      481 attatctttt tagacgaacc aacaacatat ttagatatct gtcatcaatt agaaatacta
      541 gaattagttc agaagctaaa tcaggaacaa ggttgtacaa ttgtcatggt tcttcatgat
      601 atcaaccaag cgattcgttt ctcagatcat cttattgcga tgaaagaagg ggatatcatc
      661 gctacaggtt caacagaaga cgtattaaca caggaaatat tagaaaaagt ttttaatatt
      721 gatgttgttt taagtaaaga tcctaaaact ggaaaacctt tactggtaac ttatgactta
      781 tgtcgcagag cttattctta attaagtaag ttaatatgat aaaaaggaca attaacatga
      841 caaatagaga gaacccaacg ccattgaagt ttttatccta tattataggt ttaagtatga
      901 tactactaat cacactattt atttctacat taataggtga cgccaaaatt caagcctcta
      961 caattataga ggctattttt aattataatc ctagcaatca acagcaaaac atcatcaatg
     1021 agattaggat tcccagaaat atagcagcag taattgtagg tatggcgctt gcagtttctg
     1081 gtgcgattat acaaggtgtt actcgtaatg gtcttgctga tccggcgctc ataggtttaa
     1141 attcaggtgc ttcatttgct ttagcattaa catatgcagt tttaccaaac acttcatttt
     1201 taatattgat gtttgctgga tttttaggtg ctattctagg aggtgctatt gtattaatga
     1261 taggccgatc tagacgtgat ggatttaatc cgatgcgtat tattttagcg ggtgcagcag
     1321 taagtgctat gttaacagcg ctaagtcaag gtattgcatt agcttttaga ctaaatcaaa
     1381 cagtaacatt ttggactgct ggaggcgttt caggcacaac atggtcacac cttaagtggg
     1441 caattccatt aattggtatt gcgttattca ttatattaac aattagtaaa caacttacca
     1501 ttttaaatct tggtgaatca ttagctaaag gtttaggtca aaatgtaaca atgatcagag
     1561 gcatatgttt aattattgct atgattctag caggtattgc agttgctatc gctggacaag
     1621 ttgcatttgt aggtttgatg gtacctcata tagcaagatt tttaattgga actgattatg
     1681 ctaaaattct accattaaca gccttgttag gtgggatact cgtgcttgtt gccgatgtga
     1741 tagcacgata tttaggagaa gcgcctgttg gtgcaatcat ttcatttatc ggtgttcctt
     1801 actttttata tttagttaaa aaaggaggac gctcaatatg attagttcaa ataataaacg
     1861 cagacaattg atagcactgg ctgtttttag cattctacta tttctaggtt gtacttggag
     1921 tattacctca ggtgaataca acatacctgt tgaaagattt ttcaaaactt taattggaca
     1981 aggtgatgcc attgatgagt taatcttatt agatttcagg ttacctcgga tgatgattac
     2041 tattttggct ggcgcagcgc ttagtattag tggtgcaata gtgcaaagtg tcacaaaaaa
     2101 tccaatagct gaaccaggta tattaggtat taacgcaggt ggcggatttg caatcgcatt
     2161 atttattgca attggtaaaa ttaatgctga caactttgtt tatgtactgc cgttaataag
     2221 tatactaggt ggtatcacca ctgcattgat tatttttatt ttcagtttta ataaaaatga
     2281 aggtgttaca cctgcgagta tggtattaat aggtgtaggt ttacaaacag cattatatgg
     2341 tggctcaatt acaattatgt caaaatttga tgataagcaa tctgatttca tcgctgcttg
     2401 gtttgcaggt aatatttggg gtgacgaatg gccatttgtc attgcatttt taccgtgggt
     2461 gttgattatt attccttact tactatttaa atcgaataca ctaaatatta ttcatacggg
     2521 tgataatatt gcacgaggtc taggtgtaag gttaagcaga gaacgtttaa tattattctt
     2581 tatcgcagtg atgttatcat ctgctgctgt agcagtagca ggttcaattt cgtttatcgg
     2641 attaatgggt ccgcatattg ccaaacgtat cgttggacca cgtcaccagt tgtttttacc
     2701 aattgccatt ttagtagggg catgtttact tgttatagct gatacaattg gcaaaattgt
     2761 attacaacca ggtggggttc cagcaggtat tgtcgtagca attattggtg caccgtattt
     2821 cttatattta atgtacaaaa cgaaaaatgt atagtgtcaa aggacacaac ttattgctat
     2881 gaaaggcact ttattataag gcttttcata gcatttttta tttaatgagc cactcaagac
     2941 tatttatttt ttcaataatg aaccattaag ttatcaagag gatcttatca aaaatatatt
     3001 tgataacggt atcaggttaa ttctttatga tagcgcattc atttattctg ttttatacta
     3061 tgactgataa taccaaggag gtacaacatg atgaaaaagt taatcaataa aaaagaaaca
     3121 tttttaactg atatgcttga aggattgtta attgcgcacc cagagttaga tctgattgct
     3181 aatacagtta ttgtaaaaaa agctaagaaa gaacatggtg tagcaatagt ctctggaggt
     3241 ggaagcggac atgaacctgc gcatgccggt tttgttgcag aaggtatgct agatgcagcg
     3301 gtttgtggcg aagtatttac atcacctaca cctgataaaa tattagaagc tattaaagca
     3361 gtagatactg gtgatggtgt attactagtt gtaaaaaact atgcaggtga cgtgatgaat
     3421 ttcgaaatgg cacaagagct tgcagaaatg gaaggtataa atgttcaaac tgttattgtt
     3481 cgtgacgaca ttgctgtgac aaacgaagta caacgtcgtg gtgttgcagg aacagtgttt
     3541 gttcataagc ttgccggtta tcttgctgaa aaaggttatt cattaacaga gataaaatcg
     3601 cgtgtagaag cgttgttacc tgaaattaaa agtattggta tggcaattga gccaccgctt
     3661 gttccaacta ctggaaaata tggctttgat attgaagacg acaaaatgga aatcggtatt
     3721 ggtatacatg gtgaaaaagg tattcatagg gaagaagtaa aggatattga tcatattgtt
     3781 ggaacattgt tagacgaatt gtataaagaa gttactgcca atgatgtcat attaatggta
     3841 aatggtatgg gtggtacgcc gttatctgaa ttaaatatcg taactaaata tattcaacaa
     3901 aatttagctg caagaacggt taatgttgct aaatggtttg ttggtgatta tatgacatct
     3961 ttagacatgc aaggtttttc tataactatc gtgcctaata aaccagaata tttggaagca
     4021 tttttagcac caacaacaag tcaatacttt aaataagaag tgaatatgaa taaatataca
     4081 tttatgaggt ggcacaaatg aaagtgaatg atatgaaagc acgtttatta aatttagaag
     4141 aaacgtttaa aaaacatgaa tctgaattaa ctgaattaga tcgagcaatt ggtgatggtg
     4201 accacggggt taacatggtt cgtgggttta gtagtcttaa agacaaactt gatgatagct
     4261 caatgcaatc attgttcaaa tcgactggta tggcattgat gtcaaatgtt gggggtgcat
     4321 caggaccact gtatggcttt agctttgtta aaatgtctgc agtcaccaaa gatgatatgg
     4381 ataatcaaga tttcattaca ctaattcagg catttgccga agcggttgaa tcacgtggta
     4441 aagttacttt aaatgaaaag acaatgtatg atgtagtagc gcgagcagca gagaagcttg
     4501 aaaatggtga aactttaaca ttcaatgatt tacagcaatt agcagataat acaaaagata
     4561 tggtagcaac gaaaggtaga gctgcatatt ttggagaaga atcaaaaggt tatattgatc
     4621 caggtgctca aagtatggtt tatattttaa acgctttgat tggagatgaa gataatgcct
     4681 aaaattatac ttgttagcca cagtaaagaa attgcaagtg gtacaaaatc tttgttaaag
     4741 caaatggcag gtgacgttga tattatacca atcgggggat taccagatgg ttcaattgga
     4801 acttcatttg atatcatcca agaagttttg actaaattag aggatgatgc attgtgtttt
     4861 tacgatattg gatcttcaga aatgaatgta gatatggcaa ttgaaatgta tgatggtaat
     4921 catcgtgtgt taaaagttga tgcaccaatt gttgaaggca gttttatcgc agcagtaaag
     4981 ctatcaatcg gcggttcaat tgatgatgca ttagcagaaa tcaaacaatc attttagtta
     5041 aaatttacta ataatgaaaa atgtaaacct ttttcaaatg aa
//
LOCUS       DANBWH010000183         5076 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.190, whole genome shotgun sequence.
ACCESSION   DANBWH010000183 DANBWH010000000
VERSION     DANBWH010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5076
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.190"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(138..245)
                     /locus_tag="PPY92_002238"
     CDS             complement(138..245)
                     /locus_tag="PPY92_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626218.1"
                     /translation="MVLLAFVTTIHDTDDGRFFNEHKHANNKLLKHSLI"
     gene            complement(282..605)
                     /gene="isdG"
                     /locus_tag="PPY92_002239"
     CDS             complement(282..605)
                     /gene="isdG"
                     /locus_tag="PPY92_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdG"
                     /protein_id="HDJ2626219.1"
                     /translation="MKFMAENRLTLTKGTAKDIIERFYTRHGIETLEGFDGMFVTQTL
                     EQEDFDEVKILTVWKSKQAFTDWLKSDVFKAAHKHVRSKNEDESSPIINNKVITYDIG
                     YSYMK"
     gene            complement(624..1358)
                     /gene="srtB"
                     /locus_tag="PPY92_002240"
     CDS             complement(624..1358)
                     /gene="srtB"
                     /locus_tag="PPY92_002240"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HDJ2626220.1"
                     /translation="MRMKRFLTIVQILLVVIIIIFGYKIVQTYIEDKQERANYEKLQQ
                     KFQMLMSKHQEHVRPQFESLEKINKDIVGWIKLSGTSLNYPVLQGKTNHDYLNLDFER
                     EHRRKGSIFMDFRNELKNLNHNTILYGHHVGDNTMFDVLEDYLKQSFYEKHKIIEFDN
                     KYGKYQLQVFSAYKTTTKDNYIRTDFENDQDYQQFLDETKRKSVINSDVNVTVKDKIM
                     TLSTCEDAYSETTKRIVVVAKIIKVS"
     gene            complement(1420..2385)
                     /gene="isdF"
                     /locus_tag="PPY92_002241"
     CDS             complement(1420..2385)
                     /gene="isdF"
                     /locus_tag="PPY92_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin ABC transporter permease protein IsdF"
                     /protein_id="HDJ2626221.1"
                     /translation="MIKNKKKLLFLCLLVILIATAYISFVTGTIKLSFNDLFTKFTTG
                     SNEAVDSIIDLRLPRILIALMVGAMLAVSGALLQAALQNPLAEANIIGVSSGALIMRA
                     LCMLFIPQLYFYLPLLSFIGGLIPFLIIILLHSKFRFNAVSMILVGVALFVLLNGVLE
                     ILTQNPLMKIPQGLTMKIWSDVYILAVSALLGLILTLLLSPKLNLLNLDDIQARSIGF
                     NIDRYRWLTGLLAVFLASATVAIVGQLAFLGIIVPHVVRKLVGGNYRVLIPFSTVIGA
                     WLLLVADLLGRVIQPPLEIPANAILMIVGGPMLIYLICQSQRNRI"
     gene            complement(2398..3276)
                     /gene="isdE"
                     /locus_tag="PPY92_002242"
     CDS             complement(2398..3276)
                     /gene="isdE"
                     /locus_tag="PPY92_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HDJ2626222.1"
                     /translation="MRIIKYLTILVISVVILTSCQSSSSQESTKSGEFRIVPTTVALT
                     MTLDKLDLPIVGKPTSYKTLPNRYKDVPEIGQPMEPNVEAVKKLKPTHVLSVSTIKDE
                     MQPFYKQLNMKGYFYDFDSLKGMQKSITQLGDQFNRKAQAKELNDHLNSVKQKIENKA
                     AKQKKHPKVLILMGVPGSYLVATDKSYIGDLVKIAGGENVIKVKDRQYISSNTENLLN
                     INPDIILRLPHGMPEEVKKMFQKEFKQNDIWKHFKAVKNNHVYDLEEVPFGITANVDA
                     DKAMTQLYDLFYKDKK"
     gene            complement(3273..4349)
                     /gene="isdD"
                     /locus_tag="PPY92_002243"
     CDS             complement(3273..4349)
                     /gene="isdD"
                     /locus_tag="PPY92_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated surface determinant protein IsdD"
                     /protein_id="HDJ2626223.1"
                     /translation="MRNVKQIATKSIIAIISLCILTYTTMIGSVLADEIKYPSAKFNQ
                     PEAKDKTELTTSIFDEKIKENKALELLIFNQENKNVTEEQQLVDEKAQLISDMTGKIY
                     LQVKLKGQIDKEQLVFQNDKNEEFPFVIKDEKDDTIVRILIEQHMDKINMHVKTLAEK
                     KNLDNKEMVYSIHFKEKKVQHDDAKEVPSKHQNQENNQDQLKKDIDDKKDSQKSDTKE
                     RRTSLFTEKGLNDIPVQKDKVQQDSNKKIENERPKASGTLKVENSPPTIKKVENNHKE
                     QPKHKDEKSKKEKKKVVEKEKALPAFNRDDDSKNSSQLSSDIKELDEPNHKKQYMLFA
                     AGIVLATILLISAHLYSRKRGNQV"
     gene            complement(4349..5032)
                     /gene="isdC"
                     /locus_tag="PPY92_002244"
     CDS             complement(4349..5032)
                     /gene="isdC"
                     /locus_tag="PPY92_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdC"
                     /protein_id="HDJ2626224.1"
                     /translation="MKNILKVFNTTILALIIIIATFSNSANAADSGTLNYEVYKYNTN
                     DTSIANDYFNKPAKYIKKNGKLYVQITVNHSHWITGMSIEGHKENIISKNTAKDERTS
                     EFEVSKLNGKIDGKIDVYIDEKVNGKPFKYDHHYNITYKFNGPTDVAGANAPGKDDKN
                     SASGSDKGSDGTTTGQSESNSSNKDKVENPQTNAGTPAYIYAIPVASLALLIAITLFV
                     RKKSKGNVE"
BASE COUNT         1538 a          881 c          645 g         2012 t
ORIGIN      
        1 ataatatttc aaattgcata aatttctttt atcgatacta ctactataaa tcatacgccc
       61 caaaatatca ttattaattc ttttcttctt caaaataaat caaaatgata taattgatga
      121 ttattttcaa agcacattca aatcaaacta tgttttagca atttgttgtt agcatgtttg
      181 tgttcattaa aaaaacgacc atcatcggta tcatgtatgg tcgttacaaa agctaacaat
      241 accaattgtc ataacaagta ctgcaacctc tttaaattca attatttcat gtaactatag
      301 cctatatcat atgtaattac tttgttattg ataatcgggc tactttcatc ttcattttta
      361 cttctaacat gtttatgcgc tgctttaaag acatcagatt ttaaccaatc cgtaaaagct
      421 tgctttgatt tccaaactgt taaaattttc acttcatcaa aatcttcttg ttctaaagtt
      481 tgtgtaacaa acatgccatc aaagccttct aatgtttcaa tcccatgtct cgtgtaaaat
      541 cgttctataa tatcttttgc tgttcctttt gttaacgtca gcctattttc tgccataaat
      601 ttcataatta tcctcttttc tgtttaactt accttaatta tttttgcgac aacaacaatt
      661 cttttcgttg tttcactata tgcatcttcg cacgttgata aagtcattat tttatctttt
      721 accgttacat taacatctga attaattaca gatttacgtt ttgtctcatc taaaaattgt
      781 tgataatctt gatcattttc aaaatctgta cgtatgtaat tatctttagt agtagtttta
      841 tatgcactaa atacttgcaa ttgatattta ccatatttat tgtcaaattc aattatcttg
      901 tgtttttcat aaaacgattg ctttaaataa tcttctaaca catcaaacat cgtattatca
      961 ccgacatggt gcccgtataa aatagtatta tgatttaaat tcttcaattc atttctaaaa
     1021 tccataaaaa tactaccttt acgtcgatgt tctcgctcaa aatctaaatt taaataatcg
     1081 tgatttgtct taccttgtag tactggataa tttaatgatg ttcctgataa ttttatccat
     1141 ccaacaatgt ctttatttat tttttcaagt gattcaaatt gtggtctcac atgttcttga
     1201 tgtttgctca tcagcatttg aaatttttgt tgtaatttct cataatttgc gcgttcttgc
     1261 ttgtcttcaa tatatgtttg aacaattttg taaccaaaaa tgataataat tacaaccaat
     1321 aaaatttgta caatagttaa aaatcgcttc attctcataa aaatcctctt ttattaacga
     1381 cgtttcttca gtcatcacta aaccagttgt tgtaccgttt tagattcgat ttcgttgact
     1441 ttgacaaatt aagtaaatta gcattggacc accgacaatc attaaaatag cattggctgg
     1501 aatttctaaa ggaggctgta tcactcgtcc taataaatca gccactaaca atagccatgc
     1561 accaataact gtagaaaacg gaataagtac tctgtaattg cccccaacta gctttctaac
     1621 cacatgtggc acaataatac ctaaaaaggc tagttgtcca acaatcgcaa cagttgcact
     1681 tgctaaaaat actgctaata aacctgttaa ccatctgtaa cgatcaatat taaaaccgat
     1741 acttcgcgct tgtatgtcgt ctaaatttag taaattcaat ttaggggaca atagtaatgt
     1801 taatattaat cccaataatg ctgatactgc taatatgtat acgtcgctcc atattttcat
     1861 tgttaagcct tgaggaattt tcattaaagg gttttgagtt aaaatttcta aaacaccatt
     1921 taataatacg aataacgcaa cacctactaa tatcatactt acagcattga atctaaattt
     1981 agaatgcaac aatataatta ttaaaaatgg tattaaacct ccaataaaac ttaataatgg
     2041 taagtaaaag tacaattgtg gaataaacaa catacaaagt gctctcatta taagtgcacc
     2101 tgaggaaacg ccaatgatat tcgcctctgc caaaggattt tgtagtgctg cttgtaataa
     2161 tgctccagaa actgctaaca ttgcgccaac catcaatgca attaatatac gtggcaatcg
     2221 caaatcaatg attgaatcca ctgcttcatt gctaccagtt gtaaattttg taaataggtc
     2281 attaaatgac aatttaattg taccggttac aaacgaaata taagcagttg cgattaaaat
     2341 gactaacaaa cataaaaata gtagtttctt tttatttttt atcatcatat caactcacta
     2401 ttttttatcc ttataaaata aatcatataa ttgagtcatt gccttatcag catcaacatt
     2461 tgctgtaata ccgaatggca cttcctctaa gtcataaaca tgattatttt tcacagcttt
     2521 aaaatgtttc caaatatcat tttgtttaaa ttctttttga aacattttct taacttcttc
     2581 aggcattccg tgtggtaatc gtaaaataat atctggattg atattcaaca aattttcagt
     2641 attagacgaa atatattgac gatctttcac tttaataaca ttttctccac ctgctatttt
     2701 aactaaatca ccaatatatg atttatcagt tgctactaaa tagctacccg gtacacccat
     2761 taatattaat actttgggat gtttcttttg tttagctgct ttattttcaa ttttttgctt
     2821 tacagaattt aaatggtcat ttaattcttt tgcttgtgct ttacgattaa attgatcacc
     2881 taattgtgta atcgactttt gcatcccttt taaactatca aaatcataaa agtagccttt
     2941 catatttaat tgtttgtaaa atggttgcat ttcatcttta atcgttgaca cactcaaaac
     3001 atgtgttggt tttaactttt taacagcttc aacattcggc tccattggtt gaccaatttc
     3061 cggtacatct ttataacgat taggcaatgt cttatatgac gtgggtttgc cgacaattgg
     3121 taaatccaat ttgtccaatg tcattgtcaa tgcaacagtt gttggtacga ttctgaattc
     3181 gccggattta gttgattctt gagaactgga agattgacag ctggttaaga taacgacgct
     3241 tatcactaaa atggttaaat actttatgat tctcaaactt ggttacctct ctttctgctg
     3301 tataaatgtg ccgaaataag taaaatagtt gctaacacaa tgccagctgc aaataacata
     3361 tattgctttt tatgatttgg ttcatcaagt tctttaatat cactagataa ttgactacta
     3421 ttcttgctat catcatctct attaaaagct ggtaacgctt tttctttttc aactactttt
     3481 ttcttttcct tttttgattt ttcatcttta tgtttcggtt gctctttgtg attattttca
     3541 acctttttta ttgttggagg gctattttca acttttaatg tacctgatgc tttaggtcgc
     3601 tcattttcaa tctttttatt actgtcttgt tgcactttat ctttttgtac aggaatatca
     3661 tttaatcctt tttcagtaaa aaggctagta cgtctttcct tagtatctga tttttgacta
     3721 tcttttttgt catcaatatc ttttttaagc tgatcttgat tattttcttg attttgatgt
     3781 tttgaaggca cttcttttgc atcatcatgt tgtacttttt tctctttaaa atgaatagaa
     3841 tacaccattt ctttgttatc tagatttttc ttttcagcca acgttttaac atgcatattg
     3901 attttatcca tatgctgttc aattaaaatt cttactattg tgtcatcctt ttcatctttt
     3961 ataacaaaag gaaattcttc atttttgtca ttttgaaaaa caagttgttc tttatctatt
     4021 tgacctttta gctttacttg caagtaaatt ttaccagtca tatctgaaat caattgcgcc
     4081 ttttcgtcaa ctagttgttg ttcctcagtt acatttttat tttcttgatt aaaaattagt
     4141 aactctaacg ctttattctc ttttatcttt tcatcaaaaa ttgatgtagt taattctgtt
     4201 ttatcttttg cttcaggttg attaaatttg gctgatggat attttatctc atcagccaac
     4261 acgctaccaa tcattgttgt atatgtaagt atacataagc taataatagc tataatagat
     4321 tttgtagcaa tttgtttaac atttctcatt attccacatt gcctttagat ttttttctaa
     4381 caaacaatgt gattgcgatt aataatgcta aggatgcaac tggtattgca tatatatatg
     4441 caggtgtacc agcatttgtt tgtggatttt ctactttgtc tttattcgaa ctattagatt
     4501 cactttgacc agtagtcgtt ccatcagatc ctttgtcact acctgaagca gaatttttat
     4561 catctttacc tggtgcatta gcacctgcta catcagttgg tccattaaat ttatatgtaa
     4621 tgttgtaatg atggtcatat ttgaatggct ttccatttac tttttcatcg atataaacgt
     4681 caatttttcc atctatttta ccgttcaact tacttacttc aaattcagaa gtgcgttcat
     4741 ctttggcagt gtttttacta ataatatttt ctttatgtcc ttcgatactc attccagtaa
     4801 tccaatgact gtggttgaca gttatttgaa catacaattt accatttttc ttaatgtact
     4861 ttgccggttt attaaaatag tcattagcaa ttgacgtgtc attggtattg tatttgtaaa
     4921 cctcataatt caaagtaccg ctatctgcgg catttgcaga attactgaat gtcgcgatga
     4981 tgataattaa cgctaaaatc gttgtattaa aaacttttaa aatatttttc aaaacataat
     5041 cctccttttt atgattgctt ttaagtcttt agtaaa
//
LOCUS       DANBWH010000184         5010 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.191, whole genome shotgun sequence.
ACCESSION   DANBWH010000184 DANBWH010000000
VERSION     DANBWH010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.191"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            119..1105
                     /locus_tag="PPY92_002245"
     CDS             119..1105
                     /locus_tag="PPY92_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDJ2626225.1"
                     /translation="MFIIKSMLYRLMQMIVVLFVISTLTFILMKLSPGNPVDKILHLD
                     VAQVSTEQINATKDKLGLNDSLLVQWWHWMNHLLHFNLGKSFESKEPVTQILFNYAPI
                     TLLISFSTLVVSLCISIPLGIIAAKRFHKWTDKVIRVISTLSISLPAFFIGIILLFIV
                     TNLMNIDSVILSQFILPVITLSLGMCAYIIRLVRSNLLMLLQSNIVQASRLRGMNERY
                     ILIHDLLKPTILPIIPLLGISLGSLIGGTVVIENLFDIPGIGYLLMDSIKSRDYPVIQ
                     GCVLFIGFFVVIINTIADLLTLLLDPKQRLQLGNPKNKTNTPLISESSDRHA"
     gene            1098..1928
                     /locus_tag="PPY92_002246"
     CDS             1098..1928
                     /locus_tag="PPY92_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDJ2626226.1"
                     /translation="MHKIFSKNNLIFFVFVAFIFVVIVLQFFVSSENATKVNLSQTFE
                     PISWLHLLGTDDYGRDLFTRIIIGARSTLFVTVLTLIAIVVIGVTLGLFAGYKKGWIE
                     RLVLRFIDVGLSIPEFIIMIALASFFQPSLWNLVISITVIKWMNYTRLTRSIVNSEMN
                     KPYIKMAQLFHVPTRTILIRHLTPKIIPAIIVLMVVDFGKIILYISSLSFIGLGAQPP
                     TPEWGAMLQQGRDFISSHPIMLIAPASVIAITILIFNLTGDALRDRLLKQRGEYDESH
                     "
     gene            1915..2688
                     /locus_tag="PPY92_002247"
     CDS             1915..2688
                     /locus_tag="PPY92_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDJ2626227.1"
                     /translation="MSLIDIQNLTIKNTCEKYLIKGIDLKIFSQQINALIGESGAGKS
                     LIAKALLEYLPFDLSCTYDSYQFDGENISRLSQYYGHTIGYISQNYAESFNDHTKLGK
                     QLTAIYRKHYKSSKEEALSKIDKALSWVNLQSKDILNKYSFQLSGGQLERVYIASVLM
                     LEPKLIIADEPVASLDALNGNQVMDLLQHIVLEHGQTLFIITHNLSHVLKYCQYIYVL
                     KEGQIIERGNINHFKYEHLHPYTERLIKYRTQLKRDYYD"
     gene            2681..3382
                     /locus_tag="PPY92_002248"
     CDS             2681..3382
                     /locus_tag="PPY92_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDJ2626228.1"
                     /translation="MIELKHVTFGYNKKQMVLQDINITIPDGENVGILGESGCGKSTL
                     ASLVLGLFKPVKGEIYLSDNAVLTIFQHPLTSFNPDWTIETSLKEALYYYRGLTDNTA
                     QDQLLLQHLSTFELNAQLLTKLPSEVSGGQLQRFNVMRSLLAQPRVLICDEITSNLDV
                     IAEQNVINILKAQTITNLNHFIVISHDLSVLQRLVNRIIVLKDGMIVDDFAIEELFNV
                     DRHPYTKELVQTFSY"
     gene            3506..3979
                     /locus_tag="PPY92_002249"
     CDS             3506..3979
                     /locus_tag="PPY92_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HDJ2626229.1"
                     /translation="MGLLDIASIRSIERGFNYYQSECVINLKSFSETQHEAEVKGSGN
                     KVYRCYIDMEHPRKSKCNCPHADGRRVICKHMIALLFTASPEAANKHIMMLNEVEEDY
                     QLRRNMWIDSLKEMINDMSEEELRDAYLNMLIEHGEMAELFGLDEEEEMFEDEFY"
     gene            complement(4027..4794)
                     /locus_tag="PPY92_002250"
     CDS             complement(4027..4794)
                     /locus_tag="PPY92_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDJ2626230.1"
                     /translation="MRFVFDIDGTLCFDGRLIDQTIIDTLLQLQHDGHELIFASARPI
                     RDLLPVLPSVFHQHTLIGANGAMISQQSKISVIKPIHTDTYHHILKIIQKYELDYIID
                     DDWNYAAQLDAENAIFERLDPHKLASCIDVANIDTPIKIILLNIDPAQITTILDELNK
                     YHQELEMIHHSNEYNIDITAQNINKYTALQYIFDADVKYIAFGNDHNDIFMLQHASSG
                     YIIGPSEAYTHAILKLDKIKHINNNTQAICKVLKSYK"
BASE COUNT         1688 a          695 c          880 g         1747 t
ORIGIN      
        1 atttctttat ggataatact ataatgaacg ttggttctta atatgtaagt tgcatataca
       61 tgcgccattc tcatgtaaaa agaaagtgtt agtaacttac acaaggaggg ggactattat
      121 gttcataata aagtctatgt tgtatagact gatgcaaatg atagttgttt tatttgttat
      181 ttctacactt acattcattt taatgaaatt atcaccaggc aatcctgttg ataaaatatt
      241 acatcttgat gtagcacaag tgtcgacgga acaaataaat gcaaccaaag ataaattggg
      301 attaaatgat tctttattgg tacagtggtg gcattggatg aatcatttat tacattttaa
      361 tttaggaaaa agttttgaat caaaagaacc ggtaacacag atattattca actatgcacc
      421 cataacatta ctcatatcat tttcaacatt agtagtgtcg ttatgtatat ctataccgct
      481 cggcattata gcagccaaac gatttcataa gtggacggat aaagtgatta gagtgatctc
      541 aacactatcg attagtttac cagcgttctt tatcggtatt attttattat ttattgtcac
      601 aaatttaatg aatatagata gcgttatact aagtcagttt atattacctg taatcacgct
      661 atctttaggt atgtgtgcat acatcattcg tttagtgcgt tctaatttat tgatgttatt
      721 gcaaagtaat atcgtacaag catcaagatt acgcggtatg aatgaacgtt atattttaat
      781 tcatgattta ctaaaaccaa caattttgcc gattatccca ttactaggga tttcacttgg
      841 cagtctaata ggtggtactg tagtgattga aaatttattt gatatacctg gtattggtta
      901 tctattaatg gatagtatta aatctcgaga ttatcctgtt attcaaggat gcgtgttatt
      961 tattggcttc ttcgttgtta ttatcaatac gattgctgat ttattaacgt tattacttga
     1021 tccgaagcag cgtttacaat taggaaatcc aaaaaacaaa accaatacac cattgatatc
     1081 agaaagtagt gaccgtcatg cataaaatat tttcaaagaa taacctgata ttttttgtat
     1141 tcgttgcatt tatttttgtg gtaattgtac tgcaattctt tgtcagtagt gaaaatgcaa
     1201 ccaaagtcaa tttatcacaa acttttgaac ctattagttg gttgcattta ttaggaactg
     1261 atgattatgg gagagattta tttacccgaa ttattatcgg tgcacgttca acattgtttg
     1321 ttactgtttt aacattaata gctatcgttg tcataggtgt tacactaggt ctatttgccg
     1381 gatataaaaa agggtggatt gaacgattag tgttaaggtt tattgatgtt ggtctaagta
     1441 ttccagaatt tatcatcatg attgctttag caagcttttt tcaaccatct ttgtggaatt
     1501 tagttatctc aattacagta ataaaatgga tgaattacac aaggttgact agaagtatag
     1561 ttaatagcga aatgaataag ccttatataa aaatggcaca attatttcat gtacctacaa
     1621 gaacaatatt aatacgtcat ttaacaccta aaattatacc ggctattatc gttttgatgg
     1681 tcgttgattt cggtaaaatc attttatata taagttcact atcatttatt gggttaggtg
     1741 cacaaccgcc aacaccagag tggggcgcta tgttgcaaca aggtcgtgat tttatttcgt
     1801 ctcatccaat tatgttgatt gcacctgctt cagtcattgc tataactatt ttaattttta
     1861 atttaaccgg agatgcgcta agagatagat tgctaaaaca acgaggtgaa tatgatgagt
     1921 ctcattgata tacaaaattt aacaataaag aatacttgtg agaaatatct tattaaaggg
     1981 attgatttga aaatttttag tcaacagatt aatgccttga ttggagagag cggcgctggg
     2041 aaaagtttga ttgctaaagc cttacttgaa tatttaccat ttgatttaag ctgcacgtat
     2101 gattcgtacc aatttgatgg agaaaatatt agtagattga gtcaatatta tggtcataca
     2161 attggttata tttctcaaaa ttatgcagaa agttttaacg accatactaa attaggtaaa
     2221 cagttaactg cgatttatcg taagcattat aaaagtagta aagaagaggc tttgtcaaaa
     2281 attgacaagg ctttgtcgtg ggttaattta caaagcaaag atatattaaa taaatatagt
     2341 ttccaacttt ctgggggcca acttgaacgc gtttacatag caagcgttct catgttggag
     2401 cctaaattaa tcattgcaga cgaaccagtt gcatcattgg atgctttgaa cggtaatcaa
     2461 gtgatggatt tattacagca tattgtatta gaacatggtc aaacattatt tattatcaca
     2521 cataacttaa gtcatgtatt gaaatattgt cagtacattt atgttttaaa agaaggtcaa
     2581 atcattgaac gaggtaatat taatcatttc aagtatgagc atttgcatcc gtatactgaa
     2641 cgtctaatta aatatagaac acaattaaag agggattact atgattgagt taaaacatgt
     2701 gacttttggt tataataaaa agcagatggt gctacaagat atcaatatta ctatacctga
     2761 tggagaaaat gttggtattt taggcgaaag tggctgtggt aaaagtacgc tcgcttcatt
     2821 ggttcttggc ttgtttaaac ctgttaaagg agaaatttac ttaagtgata atgctgtgtt
     2881 aacgattttc caacaccctt taactagctt taaccctgat tggacgattg agacctcatt
     2941 aaaagaagcg ttatattatt acagaggcct aactgataat actgctcagg atcaattatt
     3001 attacaacat ttatctactt ttgagttaaa cgcgcaatta ttgactaaat taccaagcga
     3061 agtgagtggc ggacaattac aaagatttaa tgtcatgcgt tcgttattag cacagcctcg
     3121 cgttttaata tgtgatgaga taacttcaaa tttagatgtt atagctgaac aaaatgtaat
     3181 caatatatta aaagcgcaaa cgattacgaa cttaaatcat tttatcgtta tttctcatga
     3241 tttatccgtg ttacaacgct tagttaatag aattatcgtt cttaaggatg gcatgatagt
     3301 cgatgatttt gcaatagagg aattatttaa tgttgataga cacccttata caaaagaatt
     3361 agtgcaaaca ttttcatatt agctatttaa gaatgcgata attctagact tgttataaaa
     3421 tatagatgaa tcaagtatat taatctagac acttatcgat tttattttct ttatttaaaa
     3481 ataataataa aaaggagtat cattaatggg attacttgat attgcaagta ttcgttctat
     3541 agaaagaggc tttaattatt atcaaagtga atgcgtcatt aacttaaaat cattttcaga
     3601 aacgcagcat gaggctgaag taaagggcag tggcaacaaa gtatatcgtt gttatattga
     3661 tatggaacat cctagaaaat ccaaatgtaa ttgtcctcat gctgatggaa gacgagtgat
     3721 atgtaaacat atgattgcat tactctttac agctagtcca gaagcagcaa ataaacatat
     3781 aatgatgtta aacgaagttg aagaagacta tcaattacgc agaaatatgt ggattgatag
     3841 tcttaaagaa atgattaatg atatgagtga agaagaactc cgcgatgcat atttaaacat
     3901 gttaattgaa catggagaaa tggcagaatt atttggatta gatgaagagg aagaaatgtt
     3961 cgaggacgaa ttttattaaa atagccccta tcgattgata atgattatca tttgataggg
     4021 gtgcttttat ttatatgatt ttaagacttt gcaaatagct tgtgtattat tattgatgtg
     4081 tttgatttta tcaagtttca atattgcgtg tgtgtatgct tctgatggtc ctataatata
     4141 gccactacta gcatgttgta acatgaaaat atcattgtgg tcattaccaa atgctatata
     4201 tttaacatct gcatcaaata tatattgtaa tgcagtatat ttattaatat tttgcgctgt
     4261 tatatcaatg ttatactcat ttgaatggtg aatcatttcc aattcttgat ggtatttatt
     4321 tagctcgtct aatatagttg taatttgtgc cgggtctata tttaataaaa tgatcttgat
     4381 tggtgtgtcg atatttgcaa catcaataca actggccagc ttatgtggat ctaaacgctc
     4441 aaaaatcgcg ttctcagcgt caagttgtgc agcataattc caatcatcat caataatata
     4501 atctaactca tacttttgaa ttatttttaa gatatggtga tatgtatctg tatgaattgg
     4561 tttgataaca gaaatctttg attgctgtga aatcatagca ccatttgcgc caattaatgt
     4621 gtgctgatga aatactgatg gtaaaactgg caacaaatca cgaatcggac gtgctgatgc
     4681 aaatataagt tcatgaccat catgttgtaa ttgtaacaat gtatcaataa tagtctggtc
     4741 aattaatcgg ccgtcaaaac aaagcgtacc atcaatatca aagacaaatc tcatcaaatc
     4801 actccaaaca atataatacc atgattatag cataagtaag ttatgtgacg atattagata
     4861 atgattatga tgtagggtac cttttgcgtc acacacatta cttcctataa tatatattat
     4921 gtaaactaga atgtgaaatt ccataagggg acttatataa cttcgatttt gtataagtcg
     4981 ttttgattat tagtaaagta tgtttgtagt
//
LOCUS       DANBWH010000185         4883 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.192, whole genome shotgun sequence.
ACCESSION   DANBWH010000185 DANBWH010000000
VERSION     DANBWH010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.192"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            61..1191
                     /locus_tag="PPY92_002251"
     CDS             61..1191
                     /locus_tag="PPY92_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HDJ2626231.1"
                     /translation="MNDNEKRVLREIYNHHNISRTQISKNLEINKATISSILNKLKYK
                     SLVNEVGEGDSTKSGGRKPILLKVNHLYGYFISLDLTYSSVEVMYNYFDGNVIKHESY
                     DLPDEKVSSILSIIKKHIDIQEKLDTYNGLLGVSVSIHGVVDNEQHVTYLPFHETEGI
                     SIAKKIKEITNVPVVVENEANLSALYERNFNHNLSYNNLIALSIHKGIGAGLIINNQL
                     YRGANGEAGEIGKTLVSKVSDNVEIFHKIEDIFSQEALLHNLSNQLNEKMTLSKLIQF
                     YNEKNPVVVEEMEQFINKIAVLIHNLNTQFNPNAIYINCPLFNEMPEILEAIKNQFKQ
                     YSRNEIQIKLTSNVKFATLLGGTLAIIQKVLQINDIYLDIKA"
     gene            complement(1305..2639)
                     /gene="cstB"
                     /locus_tag="PPY92_002252"
                     /pseudo
     CDS             complement(1305..2639)
                     /gene="cstB"
                     /locus_tag="PPY92_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532406.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide dioxygenase-sulfurtransferase CstB"
     gene            complement(2672..3736)
                     /locus_tag="PPY92_002253"
     CDS             complement(2672..3736)
                     /locus_tag="PPY92_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HDJ2626232.1"
                     /translation="MEQYKEVHINSLNKEELEKLAKMGQLIDVRTKEEYELGHINGST
                     LHPVEEIKSFNKNSNKTYYIHCKSGNRSTKACDYLAKQGYDVVNLKGGYKDYEAKNFN
                     SAPLIEKDIEIKENRKQFDFRGLQCPGPIVNISKEINNISTGEQIEVTVTDPGFNSDI
                     KSWAKQTGNTLVNLTEEANVINAIIQKEKPKEFEINDTATGTTIVLFSGELDKAVAAM
                     IIANGAKAAGKDVTIFFTFWGLNALKKAQSTRVKKKGISKMFDLMLPKDPIHMPLSKM
                     NMFGLGNIMMRYVMNKKNVDSLYSLIDQAIDQDIKLIACTMSMDVMGISKEELRDEVD
                     YGGVGTYIGHTEQANHNLFI"
     gene            3872..4132
                     /gene="cstR"
                     /locus_tag="PPY92_002254"
     CDS             3872..4132
                     /gene="cstR"
                     /locus_tag="PPY92_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide-sensing transcriptional repressor
                     CstR"
                     /protein_id="HDJ2626233.1"
                     /translation="MTYDKKMINRINRIQGQLNGVVKMMEEEKDCKDIITQLSASKGS
                     IQRLMGIIISENLIECVKTAEENNESSQELINEAVNLLVKSK"
     gene            4132..4776
                     /locus_tag="PPY92_002255"
     CDS             4132..4776
                     /locus_tag="PPY92_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDJ2626234.1"
                     /translation="MDIANMTIMLLIGVLGGFISGLIGIGGAIIIYPAILILPPLIGI
                     PAYSAYIASGLTSSQVFFSTLSGSLNARKQPAFSPKLVIYMGGGMLIGSMLGAILASL
                     FNATFVNTVYVIIAILALILMFIKVKPTTQETKSKPLLFIIVGFGIGVISGIVGAGGA
                     FIIIPVLLALFKLPMNTVVNNSIAIAFISSVGAFFIKLMQGYIPVESAIFLIIE"
     gene            complement(4761..4883)
                     /locus_tag="PPY92_002256"
                     /pseudo
     CDS             complement(4761..4883)
                     /locus_tag="PPY92_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911187.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
BASE COUNT         1629 a          720 c          778 g         1756 t
ORIGIN      
        1 gataaactat aaactaaatt caaaataact tatggggtag gcaattatgg aaaataaaat
       61 ataaatgata atgaaaaaag agtgctaagg gaaatttata accatcataa tatttcgcgt
      121 actcaaatat ctaaaaatct tgagattaat aaggcaacga tttctagtat tttgaataag
      181 ttaaagtata aatctcttgt taatgaggtt ggtgagggtg atagcacgaa gagtggtggt
      241 agaaaaccta ttcttctgaa ggttaatcat ctttatggtt attttatttc tttggattta
      301 acttatagtt ctgttgaagt gatgtacaat tattttgatg gtaatgtcat taagcatgaa
      361 tcttatgatt tacctgatga aaaggttagt agtatattaa gcataataaa aaaacatatt
      421 gatattcagg agaaacttga tacttataac ggactattag gtgtgtctgt ttctatacat
      481 ggagttgtgg ataatgagca gcatgtgaca tatttaccat tccatgaaac tgaaggaatt
      541 tcaattgcta agaaaataaa agaaattact aatgttccag tcgtagttga aaatgaagcg
      601 aatctttcag cgttatatga acgtaatttt aatcataatt tatcctacaa taatcttatt
      661 gctttaagta tacataaagg tattggtgct gggcttatta ttaataatca attgtatcgt
      721 ggtgcaaatg gggaagcggg tgaaattgga aaaacacttg tctcaaaagt tagcgataat
      781 gtggagatct ttcataagat tgaagatatt ttttcacaag aagctttact gcataattta
      841 agtaatcaac taaatgagaa gatgacgctt agcaaattaa ttcaatttta taatgaaaaa
      901 aatccagtcg tagttgaaga aatggaacaa tttataaata aaattgctgt tttaatacat
      961 aatttaaaca cccagtttaa tccgaatgca atttacatta actgtccatt gttcaatgaa
     1021 atgcctgaaa tattagaagc aattaagaac cagttcaaac aatattcacg taacgaaatt
     1081 caaataaagt taacatctaa tgtcaaattt gcaactttgc taggtggtac attagcaatt
     1141 atccaaaaag tactacagat taatgatatt tacttagata taaaagcata aaaaactaat
     1201 tcaaatgaat aatcaaagtt cgtaattgtc tttataaaaa aatccctcaa tccgaattga
     1261 attttcggat tgagggattt ttatagttct attgcagaag aaaactattt taaaaatgct
     1321 ggtaaatgtt gatagccacc tctaacgtta acaatattcg taaatccttt atattctaat
     1381 attcctaccg ctattgaact tctaacacct gattgacaat gtacataaat taggtcattt
     1441 ttatcgaaag gtatatcttc atttaaaagt ttaccgtgag gaatatgaat tgcttgtttt
     1501 aaatgacctt tacgccattc atcatcatta cgaacatcta atacattatg ttcttcacca
     1561 gtcatttcag aactatgaat agatgatgtg acgatatttg tttgtggcaa acggtaactt
     1621 tttacatttt caaaaccaat taattgtaaa gcatgaatag cttgttgaac ggtagattta
     1681 tcgccaatta attcaatatc ttagtcataa tctaaatacc aaccaatttg atttataaaa
     1741 gttttattaa aaggaatatt gatagttcca tgcatatgac caccatggaa tgcttcttta
     1801 cttcgaagat caaaagcagt ttgtgtattg cttgaactag ggtaaacatt atatggttgg
     1861 tacatttgca taccaaattg atttattttt ttcatttgtg aaaaatggtg tggtggagct
     1921 ggctgattga gtgttaaagt ttcgataaat gaagtttcat ctttaacatt aaaagcccag
     1981 ttgtttattt tctcataacc caaagtagtt gtaggtaatg aacctagcgc tttaccacaa
     2041 ggactccctg caccatgacc tggccaaatt tgaatatagt ctggtaatgt tgcagcaaat
     2101 tgtatggact gatacatttg ttttgctccg atttttgtag aaccttcaac atttacagct
     2161 ttttctaata gatctggtct acctacatca ccaacaaaga tgaagtcacc gctaaataat
     2221 cccattggta tactggaacc cccaccttcg tcagtaagta aaaaactaat actctcaggg
     2281 ggatggcctg gagtgtgtaa gacttctaat ttaatctttc ctaaatagat aatatcttga
     2341 tgcttaacga aatgtgtttg tttaggcata tttttataat taaattcatc tttaccttca
     2401 tcagatacgt atatacttgc attcaattta tttgccacat ctctaatacc tgaagcaaaa
     2461 tcagcatgaa tatgtgtttc tgcagcttta gtaattgtga atccttcttt atctgcaact
     2521 tttaaatatt ttgttaaatc tcgtataggg tcaataatca ttgcttctcc tgtacgttga
     2581 catccaatta aataagatgc ttgtgaaaaa ttgtcttcat aaaattgttt gaaaaacaaa
     2641 aaaaactcct ttttaaaata gattttattg attagataaa taagttatga tttgcttgct
     2701 cagtatgtcc aatataagta cctacgccac cataatcgac ttcatctctt aattcttctt
     2761 ttgaaattcc cataacatcc atactcatgg tacaagcaat taactttata tcttgatcga
     2821 ttgcttgatc gataagtgag tataaagaat caacattttt cttgttcatt acataacgca
     2881 tcataatatt acctagtcca aacatattca tttttgataa tggcatatgt attggatcct
     2941 taggtaacat aaggtcaaac attttggaaa tacctttctt tttaacgcga gttgattgcg
     3001 ctttttttaa tgcgttgagg ccccaaaaag taaagaaaat agttacatct ttacctgctg
     3061 ctttagcgcc atttgcgatg atcattgctg ctactgcctt atctaactca ccgctaaata
     3121 aaacaattgt tgtacctgta gcagtgtcat tgatttcaaa ttcttttggc ttttcttttt
     3181 gaataattgc attaattaca tttgcttctt cagtaagatt tacaagggta ttccctgttt
     3241 gtttcgccca acttttaata tcactattga aaccaggatc tgtaactgtt acctcgattt
     3301 gctcacccgt tgaaatattg ttaatttctt tactgatatt aacaataggt ccagggcact
     3361 gaagacctct aaaatcaaat tgtttacgat tctctttgat ttcaatatct ttttctatta
     3421 aaggagcact attgaagttc tttgcttcat aatctttata tccacccttt aaattcacga
     3481 catcataacc ttgtttggct aaataatcgc aagctttagt gcttcggtta ccgcttttac
     3541 aatgtatata atacgttttg ttgctattct tattgaatga tttaatctct tctactgggt
     3601 gtaaagttga accgttaatg tgtcctaatt catattcttc ttttgttcta acatcaatca
     3661 attgacccat ttttgccaat ttttctaatt cttctttgtt taatgaatta atgtgtactt
     3721 ctttgtattg ttccatactt acctcctata aatacctatg agggtataat aaaacggata
     3781 gaatcatttg ccaaatacct ataggggtat ttgacaattt tgttttaatt tattattatt
     3841 aacttaatca atttatgtgg aggaaatgaa tatgacttat gataaaaaaa tgattaatcg
     3901 tataaataga atacaaggtc aattaaatgg tgtcgtaaaa atgatggaag aagaaaaaga
     3961 ttgcaaagat ataattacgc aacttagtgc atctaaaggt tctatacaac gtttaatggg
     4021 gattataatt agtgaaaatt taatagaatg cgttaaaaca gcagaagaaa ataatgaaag
     4081 ttctcaagaa ttaattaatg aagcagttaa tttattagtt aaaagtaaat aatggatata
     4141 gcaaatatga ctattatgtt gctaattggc gtactgggtg gatttatatc tggattaata
     4201 ggtattgggg gcgcaattat tatttaccca gctattctta tattgccacc attaataggt
     4261 atacctgcgt atagtgcata tattgcttcg ggacttacct ctagtcaagt atttttcagt
     4321 acacttagtg gatcattaaa tgcaagaaaa caaccagctt tctctcctaa acttgttata
     4381 tatatgggag ggggtatgtt gattggaagc atgttagggg caattttagc tagtttgttt
     4441 aatgctactt ttgtaaatac ggtatatgta ataatcgcca tacttgcttt aatattgatg
     4501 tttattaaag ttaaacctac tacacaagag acgaaatcta aacctttgct atttattata
     4561 gttggatttg gaattggtgt aatttcggga attgtgggtg caggtggagc atttatcatc
     4621 attcctgtat tattagcatt atttaaatta ccaatgaata cggtagtgaa caatagcata
     4681 gcaattgctt ttatatcttc agtaggggca ttttttataa aattaatgca aggatatata
     4741 ccagtagaaa gtgcaatttt tttgataatt gaatgatgat cattttctga aaatattatg
     4801 tggtcatata atataatgcc catcatttca ctaatctctt ttattctctg agttattttg
     4861 atatctcctg gagaaggtgt gat
//
LOCUS       DANBWH010000186         4872 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.193, whole genome shotgun sequence.
ACCESSION   DANBWH010000186 DANBWH010000000
VERSION     DANBWH010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.193"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..258
                     /locus_tag="PPY92_002257"
     CDS             <1..258
                     /locus_tag="PPY92_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDJ2626235.1"
                     /translation="VAMAKVIKAFKLKPDCHCTSKYLNNLIEQDHRHIKVRKTRYQSI
                     NTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIMLAS"
     gene            451..1221
                     /gene="aadD1"
                     /locus_tag="PPY92_002258"
     CDS             451..1221
                     /gene="aadD1"
                     /locus_tag="PPY92_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-nucleotidyltransferase
                     ANT(4')-Ia"
                     /protein_id="HDJ2626236.1"
                     /translation="MRIVNGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLG
                     RQTDGPYSDIEMMCVMSTEEAEFSHEWTTGEWKVEVNFDSEEILLDYASQVESDWPLT
                     HGQFFSILPIYDSGGYLEKVYQTAKSVEAQTFHDAICALIVEELFEYAGKWRNIRVQG
                     PTTFLPSLTVQVAMAGAMLIGLHHRICYTTSASVLTEAVKQSDLPSGYDHLCQFVMSG
                     QLSDSEKLLESLENFWNGIQEWTERHGYIVDVSKRIPF"
     gene            1438..1842
                     /gene="bleO"
                     /locus_tag="PPY92_002259"
     CDS             1438..1842
                     /gene="bleO"
                     /locus_tag="PPY92_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin binding protein"
                     /protein_id="HDJ2626237.1"
                     /translation="MRMLQSIPALPVGDIKKSIGFYCDKLGFTLVHHEDGFAVLMCNE
                     VRIHLWEASDEGWRSRSNDSPVCTGAESFIAGTASCRIEVEGIDELYQHIKPLGILHP
                     NTSLKDQWWDERDFAVIDPDNNLISFFQQIKS"
     gene            2349..3611
                     /locus_tag="PPY92_002260"
     CDS             2349..3611
                     /locus_tag="PPY92_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HDJ2626238.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            3856..4629
                     /locus_tag="PPY92_002261"
     CDS             3856..4629
                     /locus_tag="PPY92_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HDJ2626239.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNL"
     gene            4642..4839
                     /locus_tag="PPY92_002262"
     CDS             4642..4839
                     /locus_tag="PPY92_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HDJ2626240.1"
                     /translation="MEEGLHRKRLISYGGLLKEIHKKLNLDDTEEGDLIHTDDDEKAD
                     EDGFSIIAMWNWERKNYFIKE"
BASE COUNT         1737 a          680 c         1054 g         1401 t
ORIGIN      
        1 gtagcaatgg ctaaagtaat taaagctttt aaacttaaac ctgactgtca ttgtacatcg
       61 aaatatctga ataacctcat tgagcaagat caccgtcata ttaaagtaag aaagacaagg
      121 tatcaaagta tcaatacagc aaagaatact ttaaaaggta ttgaatgtat ttacgctcta
      181 tataaaaaga accgcaggtc tcttcagatc tacggatttt cgccatgcca cgaaattagc
      241 atcatgctag caagttaagc gaacactgac atgataaatt agtggttagc tatatttttt
      301 tactttgcaa cagaaccgat tagatgctat aattgttatt aaaaggattg aaggatgctt
      361 aggaagacga gttattaata gctgaataag aacggtgctc tccaaatatt cttatttaga
      421 aaagcaaatc taaaattatc tgaaaaggga atgagaatag tgaatggacc aataataatg
      481 actagagaag aaagaatgaa gattgttcat gaaattaagg aacgaatatt ggataaatat
      541 ggggatgatg ttaaggctat tggtgtttat ggctctcttg gtcgtcagac tgatgggccc
      601 tattcggata ttgagatgat gtgtgtcatg tcaacagagg aagcagagtt cagccatgaa
      661 tggacaaccg gtgagtggaa ggtggaagtg aattttgata gcgaagagat tctactagat
      721 tatgcatctc aggtggaatc agattggccg cttacacatg gtcaattttt ctctattttg
      781 ccgatttatg attcaggtgg atacttagag aaagtgtatc aaactgctaa atcggtagaa
      841 gcccaaacgt tccacgatgc gatttgtgcc cttatcgtag aagagctgtt tgaatatgca
      901 ggcaaatggc gtaatattcg tgtgcaagga ccgacaacat ttctaccatc cttgactgta
      961 caggtagcaa tggcaggtgc catgttgatt ggtctgcatc atcgcatctg ttatacgacg
     1021 agcgcttcgg tcttaactga agcagttaag caatcagatc ttccttcagg ttatgaccat
     1081 ctgtgccagt tcgtaatgtc tggtcaactt tccgactctg agaaacttct ggaatcgcta
     1141 gagaatttct ggaatgggat tcaggagtgg acagaacgac acggatatat agtggatgtg
     1201 tcaaaacgca taccattttg aacgatgacc tctaataatt gttaatcatg ttggttacgt
     1261 atttattaac ttctcctagt attagtaatt atcatggctg tcatggcgca ttaacggaat
     1321 aaagggtgtg cttaaatcgg gccattttgc gtaataagaa aaaggattaa ttatgagcga
     1381 attgaattaa taataaggta atagatttac attagaaaat gaaaggggat tttatgcgtg
     1441 agaatgttac agtctatccc ggcattgcca gtcggggata ttaaaaagag tataggtttt
     1501 tattgcgata aactaggttt cactttggtt caccatgaag atggattcgc agttctaatg
     1561 tgtaatgagg ttcggattca tctatgggag gcaagtgatg aaggctggcg ctctcgtagt
     1621 aatgattcac cggtttgtac aggtgcggag tcgtttattg ctggtactgc tagttgccgc
     1681 attgaagtag agggaattga tgaattatat caacatatta agcctttggg cattttgcac
     1741 cccaatacat cattaaaaga tcagtggtgg gatgaacgag actttgcagt aattgatccc
     1801 gacaacaatt tgattagctt ttttcaacaa ataaaaagct aaaatctatt attaatctgt
     1861 tcagcaatcg ggcgcgattg ctgaataaaa gatacgagag acctctcttg tatctttttt
     1921 attttgagtg gttttgtccg ttacactaga aaaccgaaag acaataaaaa ttttattctt
     1981 gctgagtctg gctttcggta agctagacaa aacggacaaa ataaaaattg gcaagggttt
     2041 aaaggtggag attttttgag tgatcttctc aaaaaatact acctgtccct tgctgatttt
     2101 taaacgagca cgagagcaaa accccccttt gctgaggtgg cagagggcag gtttttttgt
     2161 ttcttttttc tcgtaaaaaa aagaaaggtc ttaaaggttt tatggttttg gtcggcactg
     2221 ccgacagcct cgcagagcac acactttatg aatataaagt atagtgtgtt atactttact
     2281 tggaagtggt tgccggaaag agcgaaaatg cctcacattt gtgccaccta aaaaggagcg
     2341 atttacatat gagttatgca gtttgtagaa tgcaaaaagt gaaatcagct ggactaaaag
     2401 gcatgcaatt tcataatcaa agagagcgaa aaagtagaac gaatgatgat attgaccatg
     2461 agcgaacacg tgaaaattat gatttgaaaa atgataaaaa tattgattac aacgaacgtg
     2521 tcaaagaaat tattgaatca caaaaaacag gtacaagaaa aacgaggaaa gatgctgttc
     2581 ttgtaaatga gttgctagta acatctgacc gagatttttt tgagcaactg gatccaggag
     2641 aacaaaaacg attttttgag gaaagttata aattattttc cgaacgatat ggcaagcaaa
     2701 atattgctta tgcaacagtt cataatgatg agcaaacccc tcacatgcat ttaggtgttg
     2761 tgcctatgcg tgatggaaaa ctgcaaggaa aaaatgtgtt taatcgtcaa gaactgttat
     2821 ggctacaaga taaattcccc gagcatatga aaaaacaggg ttttgagttg aagcgtggtg
     2881 aacgtggctc tgaccgtaaa catattgaga cagctaaatt taaaaaacaa actttggaaa
     2941 aagagattga ttttctagaa aaaaatttag cagttaaaaa agatgaatgg actgcttata
     3001 gcgataaagt taaatcagat ttagaagtac cagcgaaacg acacatgaaa agtgttgaag
     3061 tgccaacggg tgaaaagtcc atgtttggtt tgggaaaaga aataatgaaa acagaaaaga
     3121 aaccaaccaa aaatgttgtt atatcggagc gtgattataa aaacttagtg actgctgcga
     3181 gagataacga taggttaaaa cagcatgtta gaaatctcat gagtactgat atggcgagag
     3241 aatataaaaa attaagtaaa gaacatgggc aagttaaaga aaaatatagt ggtcttgtag
     3301 agcgatttaa tgaaaatgta aatgattata atgagttgct tgaagaaaac aagtctttaa
     3361 agtctaaaat aagcgattta aagcgtgatg tgagtttaat ctatgaaagc actaaggaat
     3421 tccttaagga acgtacagac ggcttaaaag cctttaaaaa cgtttttaag gggtttgtag
     3481 acaaggtaaa ggataaaaca gcacaattcc aagaaaaaca cgatttagaa cctaaaaaga
     3541 acgaatttga actaactcat aaccgagagg taaaaaaaga acgaagtcga gatcagggaa
     3601 tgagtttata aaataaaaaa agcacctgaa aaggtgtctt tttttgatgg ttttgaactt
     3661 gttctttctt atcttgatac atatagaaat aacgtcattt ttattttagt tgctgaaagg
     3721 tgcgttgaag tgttggtatg tatgtgtttt aaagtattga aaacccttaa aattggttgc
     3781 acagaaaaac cccatctgtt aaagttataa gtgactaaac aaataactaa atagatgggg
     3841 gtttctttta atattatgtg tcctaatagt agcatttatt cagatgaaaa atcaagggtt
     3901 ttagtggaca agacaaaaag tggaaaagtg agaccatgga gagaaaagaa aatcgctaat
     3961 gttgattact ttgaacttct gcatattctt gaatttaaaa aggctgaaag agtaaaagat
     4021 tgtgctgaaa tattagagta taaacaaaat cgtgaaacag gcgaaagaaa gttgtatcga
     4081 gtgtggtttt gtaaatccag gctttgtcca atgtgcaact ggaggagagc aatgaaacat
     4141 ggcattcagt cacaaaaggt tgttgctgaa gttattaaac aaaagccaac agttcgttgg
     4201 ttgtttctca cattaacagt taaaaatgtt tatgatggcg aagaattaaa taagagtttg
     4261 tcagatatgg ctcaaggatt tcgccgaatg atgcaatata aaaaaattaa taaaaatctt
     4321 gttggtttta tgcgtgcaac ggaagtgaca ataaataata aagataattc ttataatcag
     4381 cacatgcatg tattggtatg tgtggaacca acttatttta agaatacaga aaactacgtg
     4441 aatcaaaaac aatggattca attttggaaa aaggcaatga aattagacta tgatccaaat
     4501 gtaaaagttc aaatgattcg accgaaaaat aaatataaat cggatataca atcggcaatt
     4561 gacgaaactg caaaatatcc tgtaaaggat acggatttta tgaccgatga tgaagaaaag
     4621 aatttgtaac gtttgtctga tttggaggaa ggtttacacc gtaaaaggtt aatctcctat
     4681 ggtggtttgt taaaagaaat acataaaaaa ttaaaccttg atgacacaga agaaggcgat
     4741 ttgattcata cagatgatga cgaaaaagcc gatgaagatg gattttctat tattgcaatg
     4801 tggaattggg aacggaaaaa ttattttatt aaagagtagt tcaacaaacg ggccagtttg
     4861 ttgaagatta ga
//
LOCUS       DANBWH010000187         4862 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.194, whole genome shotgun sequence.
ACCESSION   DANBWH010000187 DANBWH010000000
VERSION     DANBWH010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.194"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            36..362
                     /locus_tag="PPY92_002263"
     CDS             36..362
                     /locus_tag="PPY92_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HDJ2626241.1"
                     /translation="MNLNIETTTQDKFYEVKVGGELDVYTVPELEEVLTPMRQDGTRD
                     IYVNLENVSYMDSTGLGLFVGTLKALNQNDKELYILGVSDRIGRLFEITGLKDLMHVN
                     EGTEVE"
     gene            364..843
                     /gene="rsbW"
                     /locus_tag="PPY92_002264"
     CDS             364..843
                     /gene="rsbW"
                     /locus_tag="PPY92_002264"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma B factor RsbW"
                     /protein_id="HDJ2626242.1"
                     /translation="MQSKEDFIEMRVPASAEYVSLIRLTLSGVFSRAGATYDDIEDAK
                     IAVSEAVTNAVKHAYKENNNVGIINIYFEILEDKIKIVISDKGDSFDYETTKSKIGPY
                     DKDENIDFLREGGLGLFLIESLMDEVTVYKESGVTISMTKYIKKEQVRNNGERVEIS"
     gene            818..1588
                     /gene="sigB"
                     /locus_tag="PPY92_002265"
     CDS             818..1588
                     /gene="sigB"
                     /locus_tag="PPY92_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigB"
                     /protein_id="HDJ2626243.1"
                     /translation="MAKESKSANEVSPEQINQWIKEHQENKNTDAQDKLVKHYQKLIE
                     SLAYKYSKGQSHHEDLVQVGMVGLIGAINRFDMSFERKFEAFLVPTVIGEIKRYLRDK
                     TWSVHVPRRIKEIGPRIKKVSDELTAELERSPSISEIANRLEVSEEEVLEAIEMGQSY
                     NALSVDHSIEADKDGSTVTLLDIMGQQDDHYDLTEKRMILEKILPILSDREREIIQCT
                     FIEGLSQKETGERIGLSQMHVSRLQRTAIKKLQEAAHK"
     gene            2022..4172
                     /locus_tag="PPY92_002266"
     CDS             2022..4172
                     /locus_tag="PPY92_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDJ2626244.1"
                     /translation="MDNQLINSIIEKYQFSKKQIEAVLTLLEEKNTVPFIARYRKEQT
                     GGLDEVQIKQIDDEYQYMVNLQKRKEEVIKNIEQQGLLTEELKKDILKQNKLQRVEDL
                     YRPFKQKKKTRATEAKRKGLEPLAIWMKARKHEVSIEEKAQQFINEEVQSVEDAIKGA
                     QDIIAEQISDNPKYRTKILKDMYHQGVLTTSKKKNAEDEKGIFEMYYAYSEPIKRIAN
                     HRVLAVNRGEKEKVLSVKFEFDTTSVEDFIARQEINHNNVNRSYILEAIKDSLKRLIV
                     PSIEREIHADLTEKAENHAIDVFSENLRNLLLQPPMKGKQILGVDPAFRTGCKLAVIN
                     PFGTFIAKGVIYPHPPVSKKEAAEKDFVQMVKAYDVQLIAIGNGTASRETEQFVADLI
                     KKHQLPVQFIIVNEAGASVYSASEIARDEFPDFQVEERSAVSIGRRVQDPLSELVKID
                     PKSIGVGQYQHDVNQKALENALTFVVETAVNQVGVDVNTASSSLLQYVSGLSSQIAKN
                     IIAYREENGAIKHNKELSKIKRLGAKTFEQSIGFLRIVDGSEPLDNTSIHPESYKVTY
                     QLLDKLGFGGNDLGSDALKAKLNSLDMDELAIELQVGVPTLEDIIKSLKAPNRDPRDE
                     FDTPILKSDVLSIEDLKEGMKLSGTVRNVVDFGAFVDIGVKQDGLVHVSKLSKKFVKN
                     PMDIVSVGDIVDVWVYSIDKNKDKVSLTMIDPHE"
     gene            4165..4620
                     /locus_tag="PPY92_002267"
     CDS             4165..4620
                     /locus_tag="PPY92_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HDJ2626245.1"
                     /translation="MNNDKLQRMVENLSEEKFGRTFRHCAYFNKRLRTTGGRYLLKSH
                     DIEINPKQYEHYGEDAVVKIILHELCHYHLHIAGKGYQHKDQDFKRLSQQVGAPRFCN
                     SIESYQQRANYEYYCTKCHAKYIRIRKVDTNRMRCGHCNGKLRMKRQLK"
BASE COUNT         1851 a          609 c          955 g         1447 t
ORIGIN      
        1 agataaatat ttttaataag taggagtgta atgaaatgaa tcttaatata gaaacaacca
       61 ctcaagataa attttacgaa gttaaagtcg gtggagaatt agatgtttat actgtgcctg
      121 aattagaaga ggttttaaca cctatgagac aagatggaac tcgtgatatt tatgttaatt
      181 tagaaaatgt gagttatatg gattcgacag gtttaggttt attcgtaggt acattaaaag
      241 cattaaacca aaatgataaa gaactataca ttttaggtgt gtcagatcgt atcggtagac
      301 tatttgaaat tactggtctt aaggatttaa tgcatgttaa tgaaggaacg gaggtcgaat
      361 aacatgcaat ctaaagaaga ttttatcgaa atgcgcgtgc cagcatcggc agagtatgta
      421 agtttaattc gtttaacact ttctggcgtt ttttcgagag ctggtgctac atatgatgat
      481 attgaagatg ccaagattgc agttagtgaa gctgtgacaa atgcagttaa acatgcatac
      541 aaagaaaata acaatgtggg cattattaac atatattttg aaattttaga agataaaatt
      601 aaaattgtta tttctgataa aggtgacagt tttgattatg aaacaactaa atcaaaaata
      661 ggtccttacg ataaagacga aaatatagac tttttacgcg aaggtggcct aggtttattt
      721 ttaatcgaat ctttaatgga tgaagtcaca gtatataaag aatctggtgt gacaatcagt
      781 atgactaagt atataaaaaa agagcaggtg cgaaataatg gcgaaagagt cgaaatcagc
      841 taatgaagtt tcacctgagc aaattaacca atggattaaa gaacaccaag aaaataagaa
      901 tacagatgca caggataagt tagttaaaca ttaccaaaaa ctaattgagt cattggcata
      961 taaatattct aaaggacaat cacatcacga agatttagtt caagttggta tggttggttt
     1021 aataggtgcc ataaatagat tcgatatgtc ctttgaacgg aagtttgaag cctttttagt
     1081 acctactgta atcggtgaaa tcaaaagata tctacgagat aaaacttgga gtgtacatgt
     1141 tccgagacgt attaaagaaa ttgggccaag aatcaaaaaa gtgagcgatg aactaaccgc
     1201 tgaattagag cgttcacctt ctatcagtga aatagctaat cgattagaag tctcagaaga
     1261 agaagtgtta gaagcaatag aaatgggaca aagttataat gcgttaagtg ttgatcattc
     1321 cattgaagct gataaagatg gttcaactgt tacgttatta gatattatgg ggcaacaaga
     1381 tgaccattat gacttaactg aaaaacgtat gattttagaa aaaatattac ctatattatc
     1441 tgatcgcgaa cgagaaatca tacaatgtac gtttattgaa ggattgagtc aaaaagagac
     1501 aggtgagcgt atcggtttaa gtcaaatgca tgtatcacga cttcagagaa cggcaattaa
     1561 gaaattacaa gaagcagcac ataaatagaa tttgcttatt aatgatacgt tttataatga
     1621 aaaatccata taattatccc ttgattatta aattgaaatc gaggggtatt tttaatttaa
     1681 ttaagatttt cgaattaata cattattaac gtagtttaat gtgtatccac ataaatgtcg
     1741 cgatatagta ttaataattt aagtgaagaa gatatctaat tgtcgtttta aataggtgga
     1801 ttgctattag aataaaaaaa gtagtcttag attatgaaat ttagaaatga tggtgtgtca
     1861 ttttcaataa tcttagtgcg ttttaaaata tagtatgacc taataattcg ttttaaatgt
     1921 tttggaagtg aaaattacat taagtatcat accttaatag aagtatttta gaatatgtta
     1981 aaataaatga gtaaatttaa gaaaaagtgt gggttaagta aatggacaat caattgatta
     2041 attcaatcat agagaaatat caatttagta aaaaacaaat tgaagcagta ttaacactgc
     2101 tagaagaaaa aaatacagta ccatttattg cgaggtatcg aaaagagcaa actggtggac
     2161 tagatgaagt tcaaataaag caaattgatg acgaatacca atatatggtc aatttacaaa
     2221 aacgtaaaga agaagttatc aaaaatatag aacagcaagg attacttact gaggaattaa
     2281 agaaggatat tttaaaacag aacaaattac aacgtgttga agacctatat aggcctttta
     2341 aacaaaagaa aaagacaagg gcaactgagg cgaaacgtaa agggttagag ccattagcga
     2401 tatggatgaa ggcacgtaaa catgaagtct caattgaaga aaaagcacaa caatttataa
     2461 atgaagaagt gcaatcggtt gaagatgcta tcaaaggtgc acaagatatt attgcggaac
     2521 aaatttcaga taatcctaaa tatagaacaa aaattttaaa agatatgtat catcaaggtg
     2581 tgttaactac atctaaaaag aaaaatgctg aagatgaaaa aggtattttt gaaatgtact
     2641 atgcatatag tgagccaatt aaacgcattg ctaatcatag agttttagct gttaatcgtg
     2701 gtgaaaaaga gaaagtatta tctgtaaagt ttgaattcga tacgacatca gtagaggatt
     2761 tcattgcacg tcaagaaatc aatcataata atgtaaatcg cagttatatt ttagaggcga
     2821 ttaaagatag cttgaaacgc ttaattgtcc cttcgataga gcgtgaaatc catgctgatt
     2881 taactgaaaa agctgaaaat catgcaatag atgtttttag tgaaaactta agaaatctat
     2941 tactgcaacc tccaatgaaa ggtaaacaaa tattaggcgt agatccagca tttagaacag
     3001 gttgtaaatt agcagtcatt aacccattcg gtacttttat agcaaaaggt gtgatttatc
     3061 cgcatccacc agtttctaaa aaagaggcag cagagaagga ttttgtacaa atggttaaag
     3121 cgtatgatgt gcaattaatt gcaattggca atggtactgc aagtcgtgaa acagaacaat
     3181 ttgttgcaga tttaattaaa aagcatcagt tgccagtaca attcatcatt gtcaatgaag
     3241 cgggcgcttc agtatactca gcatcagaaa ttgctagaga tgaatttcct gattttcaag
     3301 tggaagaaag aagtgccgta tcaattggac gacgcgttca agatccatta agtgaattag
     3361 ttaaaatcga tcctaaatct attggtgtcg gacaatacca gcatgatgtt aatcaaaaag
     3421 cactagaaaa tgcattaact ttcgttgttg aaacagcagt taaccaagta ggtgttgatg
     3481 tcaatacagc atcatcatca ttattacaat atgtgtctgg tctaagttcg caaattgcta
     3541 aaaatataat tgcatataga gaagaaaatg gcgcaataaa gcataataaa gagttaagca
     3601 agattaagcg tttgggtgct aaaacttttg agcagagtat aggattttta agaatagtag
     3661 acggttcaga gccattggat aatacatcga ttcatcccga aagctataaa gtaacgtatc
     3721 agttattaga taaactcgga tttggtggca atgatttagg tagtgatgca ttaaaagcta
     3781 aattaaattc attggatatg gatgaactgg cgattgaatt acaagtcggt gtacctacat
     3841 tagaggatat tattaaatcc ttgaaagcac ctaatcgaga cccaagggac gagtttgata
     3901 caccaatcct taaatccgat gtgctatcaa ttgaagattt aaaagaagga atgaaattga
     3961 gtggcacagt tagaaatgta gtagattttg gagcatttgt agatataggt gttaaacaag
     4021 atggacttgt ccatgtttcg aaactttcta agaagttcgt aaaaaatcca atggatattg
     4081 tcagtgttgg tgatatcgtt gacgtttggg tttacagtat tgacaaaaat aaagacaaag
     4141 tatcattaac gatgattgat ccacatgaat aatgacaaac ttcagcgaat ggttgagaac
     4201 ctttcggaag aaaaatttgg acgtacgttt cgacattgtg catattttaa taaaaggtta
     4261 cgtacaacag gcggacgtta tcttttaaag tcccatgata ttgaaattaa tccaaaacaa
     4321 tatgagcatt atggagaaga tgctgttgta aaaattatac tgcacgaatt gtgtcattat
     4381 cacttgcata ttgctggtaa aggttatcaa cataaagatc aagattttaa acgattgagc
     4441 caacaggttg gagcgcctag attttgtaac agcattgaaa gctatcaaca acgggcgaat
     4501 tatgaatatt attgtactaa atgtcatgca aaatatatac gtatccgtaa agttgatacg
     4561 aatcgtatgc gttgcggaca ttgcaatgga aaattaagaa tgaaaagaca attgaaataa
     4621 gtacaataca aatagagcta attcgtgtat atgtaagatt atttatgagt tgtatggaat
     4681 ttctatagaa tttatcagtg agttattttg attggtaaac atagtaattg aataagaaga
     4741 aattgtgttt ataaaatgtt taaagccgag attgtcgtaa tttaatactt cgcaatctcg
     4801 gcttttctac ttatgatata ggtaatggca taaattgtga cttgatagga tgaaattaaa
     4861 tt
//
LOCUS       DANBWH010000188         4809 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.195, whole genome shotgun sequence.
ACCESSION   DANBWH010000188 DANBWH010000000
VERSION     DANBWH010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.195"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            3..1283
                     /locus_tag="PPY92_002268"
     CDS             3..1283
                     /locus_tag="PPY92_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDJ2626246.1"
                     /translation="MKKLNIALLGLGTVGSGVVKIIEENRQQIQDTLNKDIVIKHILV
                     RDKSKKRPLNISQYHLTEDVNEILNDDSLDIIVEVMGGIEPTVDWLRTALKNKKHVIT
                     ANKDLLAVHLKLLEDLAEENGVALKFEASVAGGIPIVNAINNGLNANNISKFMGILNG
                     TSNFILSKMTKEQTTFEEALDEAKRLGFAEADPTDDVEGVDAARKVVITSYLSFNQVI
                     KLNDVKRRGISGVTLTDINVADQLGYKIKLIGKGIYENGKVNASVEPTLIDKKHQLAA
                     VEDEYNAIYVIGDAVGDTMFYGKGAGSLATGSAVVSDLLNVALFFESDLHTLPPHFEL
                     KTDKTREMMDSDAEINIKEKSNFFVVVNHVKGSIENFENELKAILPFHRSLRVANYDN
                     QSYAAVIVGLESSPEELITKHGYEVDKVYPVEGV"
     gene            1289..2350
                     /locus_tag="PPY92_002269"
     CDS             1289..2350
                     /locus_tag="PPY92_002269"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDJ2626247.1"
                     /translation="MRRWQGLVEEFKAHLPVNENTPKLTLNEGNTPLIHCENMSKILG
                     IDLYVKYEGANPTGSFKDRGMVMAVTKAKEQGKKIVICASTGNTSASAAAYAARAGLK
                     AIVVIPEGKIALGKLSQAVMYGAEIVSIEGNFDEALEIVKEIAKSGEIELVNSVNPFR
                     IEGQKTGSFEIVQQLDGEAPDILAIPVGNAGNITAYWKGFKEYHEAKGSQLPKMFGFQ
                     AEGASPIVQNKVIKNPETIATAIRIGNPASWDKATNALKESNGLIDSVTDDEILEAYQ
                     LMTTKEGVFSEPASNASIAGLIKLHRQGKLPQGKKVVAILTGNGLKDPDTAISLLDNP
                     IKPLQNDKDSIIDYIKGAL"
     gene            2352..3266
                     /locus_tag="PPY92_002270"
     CDS             2352..3266
                     /locus_tag="PPY92_002270"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDJ2626248.1"
                     /translation="MSNVLELTIPASTANLGVGFDSIGMALDKFLHLSVKETSGTKWE
                     YIFHDDASKQLPTDETNFIYHVAQQVASKYSVDLPILCIEMRSDIPLARGLGSSASAL
                     VGAIYIANYFGDIQLSKHEVLQLATEIEGHPDNVAPTIYGGLIAGFYNDVSKETSVAH
                     IDIPDVDVIVTIPTYELKTEASRRALPQKLTHSEAVKSSAISNTMICALAQHNYELAG
                     KLMQQDGFHEPYRQHLIAEFDEVKTIASQHNAYATVISGAGPTILIFSRKENSGELVR
                     SLNSQVVSCHSELVDINISGVKERIVYQ"
     gene            3324..4127
                     /locus_tag="PPY92_002271"
     CDS             3324..4127
                     /locus_tag="PPY92_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDJ2626249.1"
                     /translation="MTNYKVVVLDMDDTLLNSDNVISEETANYLTAIQDEGYYVVLAS
                     GRPTEGMIPTARDLKLPEHHSYIISYNGSKTINMTNEEVEVSKSIGKQDFDEIVDYCR
                     DRGFFVLTYHDGQIIYDSEHEYMNIEAELTGLPMKRVDDIKAYIQGDVPKVMGVDYVA
                     NITEARIDLNGVFNDNVDATTSKPFFLEFMTKDVSKGNAIKALCHKLGYSVDQVIAFG
                     DSMNDKSMFEVAGLAIAMGNASDELKQYADEVTLDHNENGIPHALKKLL"
     gene            complement(4420..4734)
                     /locus_tag="PPY92_002272"
     CDS             complement(4420..4734)
                     /locus_tag="PPY92_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626250.1"
                     /translation="MIGQTNLFDDVLKQDERFVIVVQALEEKNGQLLKRTLREYPGLN
                     HKQMNDLFTHLKELFSEESFAENQSAFSITVYTNLDYTADQIYAHVKRFRGKHDWTQT
                     AK"
     gene            complement(4709..>4809)
                     /locus_tag="PPY92_002273"
     CDS             complement(4709..>4809)
                     /locus_tag="PPY92_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626251.1"
                     /translation="PFFILEILHIMYNIDNNNFSGVKFNDWTNKFI"
BASE COUNT         1732 a          650 c          940 g         1487 t
ORIGIN      
        1 aaatgaaaaa attaaatata gcattattag gattaggtac tgtcggatct ggtgttgtta
       61 aaatcatcga agagaaccga cagcaaattc aagatacatt aaataaagat attgtcataa
      121 agcatattct tgttcgagat aaatctaaaa agagaccgct aaatattagc caatatcatt
      181 taactgaaga tgttaatgaa attttaaatg atgattcatt agatattatc gttgaagtca
      241 tgggaggaat tgaaccaact gtagattggt taagaacagc acttaaaaat aaaaaacatg
      301 ttattaccgc aaataaagat ttattagcag tacatcttaa acttttagaa gatttagcag
      361 aagaaaatgg tgtagcttta aagtttgaag cgagtgtagc aggtggtatt ccgatcgtaa
      421 atgccataaa taatggtttg aatgcgaata atatttcaaa atttatggga attttaaatg
      481 gtacctctaa ttttatttta tctaaaatga ctaaagagca aacgacattt gaggaagcac
      541 ttgatgaagc gaaaagactt ggttttgctg aagcggatcc aactgatgat gtagaagggg
      601 tagatgcagc gcgtaaagtt gtcattacat catatttatc atttaaccaa gtcattaaat
      661 taaacgacgt taaacgaaga ggaattagtg gcgtaacttt aactgatatt aatgtagccg
      721 atcaactggg gtataaaatt aaattgattg gtaagggaat atatgaaaat ggcaaagtta
      781 atgcatcggt agaaccaacg ttaattgata aaaagcatca attagcagct gtagaggatg
      841 aatataacgc gatttatgtt attggtgatg ccgttggtga cacgatgttt tatggaaaag
      901 gagcaggcag tttagcaaca ggtagtgccg ttgtcagtga tttattgaat gtagcattat
      961 tctttgaatc agatttacac acattgccac cacattttga attaaagaca gataaaacaa
     1021 gggaaatgat ggattcagat gcagaaatta atattaaaga aaaatccaat ttctttgtag
     1081 tagtgaatca tgtcaaaggt tcaattgaaa attttgaaaa tgagttaaag gcaatattac
     1141 catttcaccg atcattaaga gttgcaaatt acgataatca atcatatgcc gctgttatag
     1201 ttggattgga atcatcaccg gaagaattaa tcactaagca tggatacgaa gttgacaaag
     1261 tatacccagt agaaggagtt taattataat gagaagatgg caaggattag tagaagagtt
     1321 taaagcacat ttaccagtaa atgaaaatac accaaaatta acattgaacg agggaaatac
     1381 accactcatt cattgtgaaa atatgtctaa aatactaggt atagatttat atgtgaagta
     1441 tgaaggtgcc aatccgacag gttcatttaa agatcgcggt atggtaatgg ctgtgacaaa
     1501 agcaaaagag caaggtaaga aaattgtaat atgcgcttcg actggaaata catcagcgtc
     1561 tgcagcagca tatgcagcga gagcaggttt aaaagctatc gtcgtaatac cagaaggtaa
     1621 aattgcatta ggtaaattgt cgcaagcagt aatgtatggt gcagaaatcg tttctattga
     1681 aggaaacttt gatgaagctt tagaaattgt aaaagaaatt gcaaaaagtg gcgaaataga
     1741 gcttgtaaac tctgtcaatc catttagaat cgaagggcaa aagacaggct catttgaaat
     1801 tgtacaacaa ttagacggtg aagcacctga tattttagcg attcctgtag gtaatgcagg
     1861 taatattact gcatattgga aaggctttaa agaatatcat gaagctaaag gatcacaatt
     1921 gccgaaaatg tttggcttcc aagctgaagg cgcatcacca attgttcaaa ataaagtcat
     1981 taaaaatcct gaaacgattg caactgctat tcgaattggt aatcctgcta gttgggataa
     2041 ggcgactaat gctcttaaag aatcaaatgg attaatagat agtgttactg atgatgaaat
     2101 tctagaagca tatcagttaa tgacaactaa agaaggtgtc tttagtgaac cagcaagtaa
     2161 tgcttctatt gcaggtttaa ttaaattgca tagacaaggt aaattacctc aaggtaaaaa
     2221 agtagttgct attttaactg gtaatggatt aaaagatcct gatactgcta tttcactact
     2281 agataatccg ataaagccat tgcaaaatga taaagatagc attatcgatt atattaaagg
     2341 agctttataa catgtcgaat gttttggagt taacaattcc tgcatcaaca gccaaccttg
     2401 gagttggctt tgattctata ggtatggctt tagataaatt tttgcatctg tctgtaaagg
     2461 aaacatcagg gacaaaatgg gaatatattt tccatgatga tgcatctaag caattgccta
     2521 ctgacgaaac aaactttatt tatcatgtag cacaacaagt tgcttctaaa tatagtgttg
     2581 acttgcctat tttatgtatc gaaatgagaa gtgatattcc attggcaaga gggttaggtt
     2641 cgtcagcttc tgctttagta ggagctatat atatcgcaaa ttattttggt gatatccaac
     2701 tgtctaaaca tgaggtatta caattagcga ctgaaatcga aggacatcct gataatgttg
     2761 cgccgaccat ttatggtggt ttaatcgctg gattttataa tgatgtctcg aaagaaacgt
     2821 cagttgcaca tatcgacata ccagacgtgg atgtgattgt aacgatacca acttatgaac
     2881 taaaaacaga agcatcaaga cgtgctttac cacaaaaatt aacacatagt gaagcggtaa
     2941 aaagtagtgc aattagtaat acaatgattt gtgcattagc acagcacaat tatgaattag
     3001 caggtaaact catgcaacaa gatggctttc atgaaccgta tcgtcagcat ttaattgctg
     3061 aatttgatga agtgaaaaca attgctagtc aacataatgc ctatgcaact gtaattagtg
     3121 gtgctggacc aactatttta atatttagtc gtaaagaaaa tagtggggaa ttggttcgct
     3181 ctttaaatag tcaggtagta tcatgccatt ctgaattggt cgatattaat atcagtggtg
     3241 ttaaagaacg aattgtatac caatagacgc tttatattgt aaaatagtat taaatgcaga
     3301 atagagagga gatttaatgc gatatgacaa attataaagt tgtcgtttta gatatggatg
     3361 acacattgct aaattcagat aatgtgatat cagaagaaac tgcaaattat ttaacagcaa
     3421 ttcaagatga aggttattat gttgttttag catctggtag acctactgaa ggtatgattc
     3481 caactgctag agatttaaaa ttacctgaac atcatagcta tattattagt tataacggta
     3541 gtaaaacgat taacatgact aatgaagaag tagaagtaag taaatcgatt ggtaagcaag
     3601 atttcgatga aattgtagat tattgtcgag acagaggctt tttcgttctt acatatcatg
     3661 atggtcaaat tatttacgac agcgaacatg agtatatgaa tattgaagca gaattaacag
     3721 gtttaccgat gaaacgtgtt gatgatatca aagcgtatat tcaaggcgat gtacccaagg
     3781 tcatgggtgt agattatgta gcgaatatta cagaagctag aattgatttg aatggtgtgt
     3841 tcaatgataa tgtagatgct acgacaagta agccattctt cttagaattt atgaccaaag
     3901 atgtttcaaa aggtaatgca attaaagcgt tatgtcacaa attgggatat tcggtggatc
     3961 aagtcattgc ttttggtgat agtatgaatg ataaatcaat gtttgaagtc gcaggtctag
     4021 ctattgctat ggggaatgca tcagatgaac ttaagcaata tgcagatgaa gttacgttgg
     4081 accataatga aaatggtatt ccacatgcgc tcaaaaaatt gttataaatt ttaaaataag
     4141 ccttaacaca tgatatttga ataagatatc ttgtggttaa ggctttttat ttatgtgaaa
     4201 atgacttcag ttatactatg gaggatttga aatacatatt ttagattagt aatgatatca
     4261 aacgaataga gtaaatgtat attttttgaa taaatcaagt attaactagt cacggaaggt
     4321 aggtaaataa atttgatcat tctgaataat aacgaatgca ttaaaaaatg cagtgacaat
     4381 ctaacattaa ttgttaaaat cactgcaagc atcttgattt tatttagctg tttgtgtcca
     4441 gtcatgctta cctctgaaac gttttacatg agcatatatt tggtcagcag tataatctaa
     4501 atttgtataa actgtaatac tgaacgctga ttggttttca gcaaatgatt cttcggaaaa
     4561 taattccttt aagtgcgtaa ataaatcatt catttgttta tggtttaaac cgggatattc
     4621 ccttaaagtt ctctttaata attgtccgtt cttttcttct aatgcttgaa ctacaataac
     4681 aaatctttca tcttgcttta gtacatcatc aaataaattt gtttgtccaa tcattgaatt
     4741 tcacccctga aaagttattg ttatctatat tatacatgat atgcaaaatt tctaaaataa
     4801 agaatggta
//
LOCUS       DANBWH010000189         4771 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.196, whole genome shotgun sequence.
ACCESSION   DANBWH010000189 DANBWH010000000
VERSION     DANBWH010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.196"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            183..776
                     /locus_tag="PPY92_002274"
     CDS             183..776
                     /locus_tag="PPY92_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDJ2626252.1"
                     /translation="MILVIDNNDSFTYNLIDYIKTQTKLTVQVVGIDNLLIEDVINMK
                     PKAIVISPGPGNPDDYPILNEVLEQFYQRVPILGVCLGFQCIVSYFGGNIIHGYHPVH
                     GHTTQLRHTNEGIFQGLPQNFNVMRYHSLIADGATFPNCLKITAKNDEAIIMAFEHIR
                     FPVFGVQYHPESILSEYGYRQVELFLSKVGDYCENRI"
     gene            760..1911
                     /locus_tag="PPY92_002275"
     CDS             760..1911
                     /locus_tag="PPY92_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HDJ2626253.1"
                     /translation="MRIEYNYRYYLTENEYKQYHIQLKGFIKKYVATKLADVGEVIHF
                     AQAQQRQGRYVSLYLSYEAAKYFNHVMCTHSLAKDDIYAVAYSFEKAESINSTYEHQT
                     SYVSKHHFSFVESSEVMMTNIKRVQQAIVEGETYQVNYTARLTDNIYYPISTLYERLT
                     QFSNGNYTALLQTDEIQVASISPELFFQKGQFNNVDNVIISKPMKGTMPRGKTEAEDQ
                     QYYKTLQTSSKDRAENVMIVDLLRNDIGRISQSGSIKVYKLFFIEAYKTVFQMTSMVS
                     GTLKTNTDLTQILTSLFPCGSITGAPKLNTMKYIKQFESSPRGIYCGAIGLLLPTEDD
                     KMIFNIPIRTIEYKYGQAIYGVGAGITIDSKPKDEVNEFYAKTKILEML"
     gene            1911..2519
                     /locus_tag="PPY92_002276"
     CDS             1911..2519
                     /locus_tag="PPY92_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HDJ2626254.1"
                     /translation="MQLFETMKIDNGHIPRLTYHTNRIKCSSERLNFKFDEHAWRNEL
                     NDVTTKYHSGQYRLKIVLNAESKFETIVSPLPEKSSFTAKFQVLPKVVNPTFIKNKTT
                     ERKHLAHNHETDLILLTSEDGKVLEFDIGNIVIEEDGKWYTPSYKDDFLKGCMRDYLI
                     DSDKLVEKDFNKNELIYKYHNNEIRLFLINSLREVADVHLCL"
     gene            2585..2791
                     /locus_tag="PPY92_002277"
     CDS             2585..2791
                     /locus_tag="PPY92_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626255.1"
                     /translation="MIIVYIVLLLILVYVNYRLVNRLLSENRIYVVRLIVTITTVISF
                     ILVYALIHELMPFVVRAMDLMYHQ"
     gene            2879..3589
                     /locus_tag="PPY92_002278"
     CDS             2879..3589
                     /locus_tag="PPY92_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /protein_id="HDJ2626256.1"
                     /translation="MKIYSQGDQAIVVAIEKEVSKSLTEDLLTLRSYLIEQNYPFIIE
                     IVPSESDMMIVYDARDMIKHHNIQSPFLYMKALIESIHLNIKHDFNQQDLIEIPIVYG
                     SKYGPDIESLLKHYKIKLETFIELHSKTQYFVSMMGYSPGFPYLTGLNKKLYINHTSK
                     QKKFIPAGSVVLEGKKCGIVTTDTINDWLVIGYTPLSLFNPKESDFARLKLGDNIKFR
                     PINENELEVGAFKDVNHN"
     gene            3573..4577
                     /locus_tag="PPY92_002279"
     CDS             3573..4577
                     /locus_tag="PPY92_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HDJ2626257.1"
                     /translation="MSIIIEKSGLFSSFQDFGRRGYEHDGVIPCGALDTLAHEIANRL
                     VANDKNEATLEMTNKMATIRFTEPTLIALAGGNVKAYTEHMTISPYKLYLLDKGDVLK
                     FRETSYTSRVYLAVGGGFELDAWLGSNSTDFNVKIGGFKGRTLQDGDEIKLKRDYTAR
                     HHKLFENLAHTKQTDWGIDGYALSFNYMSDVFHVVKNKGTEDFKEDAIQRFVKHDYKV
                     TSKANRMGMMLEGEKIKAFYEDMPPYQTVKKGTIQIKRDGTPIILLNDHYTLGSYPQI
                     GTIASYHLTKLAQKPQGSRLKFQFIDILTAEKNLVKYSNWLNQLFHGIEYRMQLEMMK
                     "
BASE COUNT         1739 a          659 c          850 g         1523 t
ORIGIN      
        1 tcaatgtcta gttttttata gagggtttca gctagaaacc tctgatattc atttatgtac
       61 gatgaataga atataccata tattagttaa aatcaaggtc aggataatga caatgctttt
      121 aaagttttag ctacatccaa tacatattat aaaaataaaa cacatttaga acggagtata
      181 caatgattct agtcatagat aataatgatt catttacata taatttaata gactatatta
      241 agactcaaac gaaactaaca gttcaagttg ttggtattga taatctgctg atagaagacg
      301 tcattaatat gaagccaaaa gcaattgtta tttcgcctgg gccgggtaat ccggatgatt
      361 atcctatctt gaatgaagtg ttagaacaat tttatcagcg tgtacctata ctaggtgtat
      421 gtttaggatt tcaatgtatc gtgtcttatt ttggtggaaa tatcattcac ggctatcatc
      481 ctgtacacgg acatactaca cagttacgcc ataccaatga aggtattttt caaggactgc
      541 ctcaaaattt caatgtaatg cgttatcatt cattaattgc tgacggagcg acttttccaa
      601 attgcttaaa gattacagca aaaaacgatg aagcgattat tatggcattt gagcatatta
      661 gatttccggt ttttggtgtg caatatcatc ctgaatctat tttgagtgaa tacggttatc
      721 gacaagttga attattttta tcgaaggtag gtgattactg tgagaataga atataattat
      781 cgctactatt taactgaaaa tgaatataag caataccata ttcaattaaa gggatttata
      841 aagaagtatg ttgctactaa gttggctgat gtgggagaag tgatacactt tgcacaagcg
      901 cagcaacgac aaggtagata tgtctcgtta tatttaagtt acgaagcggc aaagtatttt
      961 aatcatgtta tgtgtacaca ttcattagct aaagatgata tttatgcagt agcttatagt
     1021 tttgaaaaag cggaaagcat aaattcaaca tatgaacatc aaacttctta tgtatcaaag
     1081 catcattttt catttgttga atcttctgag gttatgatga ctaatattaa acgtgtccaa
     1141 caagcaattg ttgaaggtga aacgtatcaa gtgaactata cggcgcgctt aacggataac
     1201 atttattatc ctattagtac tttatatgaa cgattaactc aatttagtaa tggtaattat
     1261 actgcgttat tacaaactga tgaaatccaa gtagcgtcta tctcaccaga attatttttt
     1321 caaaaaggac aatttaacaa tgtcgataac gttatcataa gcaaaccgat gaaagggaca
     1381 atgcctagag gtaaaacgga agctgaagat caacagtatt ataaaacatt gcaaacttct
     1441 tcgaaagatc gtgcagaaaa tgtcatgatt gttgatttgc taagaaacga tatagggaga
     1501 atatcacaga gtggctcaat taaggtgtat aaactatttt ttattgaggc atataaaact
     1561 gtatttcaaa tgacttcgat ggtaagtgga actttaaaaa ctaatacaga cttaactcaa
     1621 attttaacat cgttatttcc ttgtggttcg attacaggtg caccgaaact gaatacaatg
     1681 aaatatatta aacaatttga aagttcacct cgtggtatat actgcggagc aattggacta
     1741 ttacttccaa ctgaagatga taaaatgatt tttaatattc cgattcgtac tattgagtat
     1801 aaatatggac aagcgattta tggagtcgga gcaggtatta caattgattc taagccaaaa
     1861 gatgaagtaa atgaatttta cgcaaaaacc aagattttgg agatgttata atgcaattat
     1921 ttgaaacaat gaaaattgat aatggacata tccctagact tacttatcat actaatcgca
     1981 taaaatgttc ttctgagcga ttaaacttta aatttgatga acatgcatgg cgaaatgaat
     2041 taaacgatgt aacaacaaag tatcacagtg gtcaatatag acttaaaatc gtattaaatg
     2101 ctgaaagcaa atttgaaacg atagtgtcac ctttacctga gaaaagtagt tttacagcaa
     2161 aatttcaagt gttgcccaaa gtagttaatc caacttttat aaaaaataaa acgacagaac
     2221 gaaagcattt agcacacaat catgaaacag atttaatatt gctaacttca gaggacggca
     2281 aggtccttga atttgatatt ggcaacattg tcattgaaga ggatggaaaa tggtacacac
     2341 caagttataa agatgatttc ttaaaaggat gcatgcgtga ttatttaata gatagtgaca
     2401 aacttgttga aaaagacttt aataaaaacg aattgattta taaatatcat aacaatgaga
     2461 tacgtttatt tttgataaat agtttacgag aggttgccga tgtccacctt tgcctttaaa
     2521 attagtatga taataaagta gaaaatgatg tgaaaacaaa tttaaaaatt aaatggggcc
     2581 aaatatgatt attgtttata ttgtgctgtt gttaattctt gtatacgtaa attatcgatt
     2641 agtgaatcga ttgctatctg aaaatagaat atatgttgtt cgtttgatag taacaattac
     2701 tactgttata agctttatcc ttgtatacgc attaattcac gaacttatgc cttttgttgt
     2761 gcgggcaatg gatttaatgt accaccagta aatttagtga gtaggtaaaa cgaactattc
     2821 aatcgattct ataattaaaa atgaaagtaa agtaaccaaa tattgaagag gtgtacaaat
     2881 gaaaatatat agtcaaggtg accaagccat tgtagtcgca attgaaaaag aagtatctaa
     2941 aagtttaacc gaagatttat taacacttcg ctcatattta attgaacaaa attatccatt
     3001 tattatagaa attgtgccat cagaatcaga catgatgatt gtctatgacg caagggatat
     3061 gattaaacac cataatatac aatcaccttt tttatacatg aaagcactaa tagaatcgat
     3121 tcatttaaac ataaaacatg attttaacca gcaagatttg attgaaatac caattgtgta
     3181 tggttcgaaa tatggtccgg atatagaatc acttttaaaa cattacaaaa tcaagctaga
     3241 aacttttatt gaattacatt ctaagacgca atattttgtt tcgatgatgg gatattcacc
     3301 tgggtttcct tatttaactg gattaaataa gaaattgtat attaatcaca cgagtaaaca
     3361 gaaaaaattc attccagctg gttctgtagt acttgaaggg aaaaaatgcg gtattgtaac
     3421 aacggataca attaatgatt ggttagttat tggttataca ccattatcac tttttaatcc
     3481 gaaagaatca gatttcgcac gcttaaagtt aggcgataat attaaattta gacctatcaa
     3541 tgaaaatgaa ttagaagtag gagcgtttaa agatgtcaat cataattgaa aaaagtggct
     3601 tattcagtag ctttcaggac tttggtagaa ggggatatga acatgatggt gtaattccat
     3661 gtggtgcact tgatacttta gcacatgaaa ttgctaatcg attagttgct aatgacaaga
     3721 atgaagcaac tttggaaatg actaataaaa tggcaacgat tcgttttaca gaacctacgc
     3781 tgattgcatt agcagggggt aatgtcaaag cttacactga gcatatgact atatctccat
     3841 ataaattgta tttgttagat aaaggcgatg ttttaaagtt tagagaaaca agttatacat
     3901 cgcgagtgta tttagctgtg ggaggcggat ttgaattaga tgcatggtta ggatctaact
     3961 caaccgactt taatgtaaaa attggtggtt ttaaaggtag aacattacaa gatggcgatg
     4021 aaataaagct taagagagat tatacagctc gtcatcataa gttatttgaa aaccttgctc
     4081 acacgaaaca aacagattgg ggtattgatg gatacgcctt gtcatttaat tatatgtctg
     4141 atgtatttca tgtcgttaaa aataaaggta cggaagattt taaagaagat gccattcaaa
     4201 gatttgtgaa acatgattat aaagtaacga gcaaagcaaa tcgcatggga atgatgcttg
     4261 aaggtgaaaa aatcaaagct ttttatgaag atatgccgcc gtatcagact gtcaaaaaag
     4321 gaacgataca aattaagcgt gatggcacac ctattatctt attaaatgat cattatacgt
     4381 taggtagcta cccgcaaatc ggtacaatcg caagttatca tttaacgaaa ttagcacaaa
     4441 aaccgcaagg atcacgtttg aaatttcaat ttatagatat tttaacggct gaaaagaacc
     4501 ttgttaagta tagtaactgg ttaaaccaat tattccatgg aatagaatat agaatgcaat
     4561 tagaaatgat gaaataatat ttggtacgta gctcatactc gagtccgatg caaataattt
     4621 cctcctaatg tataatgaaa taatactgtg ttttatctgc gaaatgtatc attttctaat
     4681 tcgtttcaca gtaaaatgaa aagataaagt gtgtttttac ttgaattttg actaaaatta
     4741 ctctatattt attaattgag ctatgcttat t
//
LOCUS       DANBWH010000190         4761 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.197, whole genome shotgun sequence.
ACCESSION   DANBWH010000190 DANBWH010000000
VERSION     DANBWH010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.197"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            194..349
                     /locus_tag="PPY92_002280"
     CDS             194..349
                     /locus_tag="PPY92_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626258.1"
                     /translation="MMVKKLLKAIKQNFLYEQSNITFKMIVFGYKRLSKMLFQFEINH
                     INFLWEG"
     gene            357..878
                     /locus_tag="PPY92_002281"
     CDS             357..878
                     /locus_tag="PPY92_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626259.1"
                     /translation="MINIISAIGSIGTFIMALFYFVSVSVQLYQMKISFLPALGFNQI
                     LLEREKDQLNIMNSATEEHHHKDYIKLYNLGGGAAKKIAIEVLLGNDKVIQKKYVNIL
                     PSKEGYMLPINKNVYEELERTIENNGYEADLNVRMTYYHNVSRKQQEVILKGQIDRFN
                     TYNNKEIYDLQFI"
     gene            1017..1547
                     /locus_tag="PPY92_002282"
     CDS             1017..1547
                     /locus_tag="PPY92_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDJ2626260.1"
                     /translation="MQIRQIHQHDFAQVDQLIRTAFENSEHGYGNESELVDQIRLSDT
                     YDNNLEIVAVLQNEVVGHGLLSEVYLDNGAQREIGLVLAPVSVDIHHQNKGIGKRLIQ
                     ALEREAILKGYNFISVLGWPTYYANLGYQRASMYDIYPPYDGIPDEAFLIKELKVNSL
                     AGKTGTINYTSAFEKI"
     gene            1810..>4761
                     /gene="clfA"
                     /locus_tag="PPY92_002283"
     CDS             1810..>4761
                     /gene="clfA"
                     /locus_tag="PPY92_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfA"
                     /protein_id="HDJ2626261.1"
                     /translation="MNMKKKEKHAIRKKSIGVASVLVGTLIGFGLLSSKEADASENSV
                     TQSDSASNESKSNDSSSVSAAPKTDDTNVSDTKTSSNTNNGETSVAQNPAQQETTQSS
                     STNATTEETPVTGEATTTTTNQANTPATTQSSNTNAEELVNQTSNETTSNDTNTVSSV
                     NSPQNSTNAENVSTTQDTSTEATPSNNESAPQNTDASNKDVVSQAVNPSTPRMRAFSL
                     AAVAADAPAAGTDITNQLTDVKVTIDSGTTVYPHQAGYVKLNYGFSVPNSAVKGDTFK
                     ITVPKELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVDNKENVTANIT
                     MPAYIDPENVTKTGNVTLTTGIGTNTASKTVLIDYEKYGQFHNLSIKGTIDQIDKTNN
                     TYRQTIYVNPSGDNVVLPALTGNLIPNTKSNALIDAKNTDIKVYRVDNANDLSESYYV
                     NPSDFEDVTNQVRISFPNANQYKVEFPTDDDQITTPYIVVVNGHIDPASTGDLALRST
                     FYGYDSNFIWRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPEDSDSDPG
                     SDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASDSDSA
                     SDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSASDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSE
                     SDSDSDSDSDSESDSDSDSDSDSDSASDSDSGSDSDSSSDSDSDSTSDTGSDNDSDSD
                     SNSDSESGSNNNVVPPNSPKNGTNASNKNEAKDSKEPLPDTGSEDEANTSLIWGLLAS
                     LGSLLLFRRKKE"
BASE COUNT         1716 a          754 c          928 g         1363 t
ORIGIN      
        1 taaatgtaaa attttgattt gttaagaaat tacgtttgta aaaataaaaa aatcaactta
       61 tttgtatgag atgaatatgt attgaagaag atgtgttatt aatttggaaa atactggtca
      121 aagatgggag ctcttaaaag cgttattgta ttttttagtc aatgcaaata gattgccgta
      181 ataataatcg tacttgatgg ttaaaaaatt acttaaggct ataaagcaaa actttttata
      241 tgagcagtcg aatataacgt ttaaaatgat tgtttttgga tataaacgat taagtaaaat
      301 gctttttcag tttgaaatta atcatataaa tttcttatgg gaaggttgat atcttaatga
      361 ttaatatcat ttcagctata ggatctattg gaacatttat tatggcttta ttttattttg
      421 tatcagtttc agttcaactt tatcaaatga aaattagctt tctgccagct ttaggtttta
      481 accaaatttt attagaaagg gagaaggatc aacttaatat aatgaattcg gcaacagaag
      541 agcatcatca taaagattat attaaactat ataatttagg tggcggtgct gctaaaaaaa
      601 ttgcaataga ggttttattg ggtaatgata aagtcattca gaaaaaatac gtgaatattt
      661 tacctagtaa agaagggtac atgttaccaa ttaataaaaa tgtgtacgaa gaattagaaa
      721 gaacgattga gaataatggt tatgaagctg atttgaatgt acgtatgact tattatcata
      781 atgtaagtcg caaacaacag gaagttatat taaaaggtca aatcgaccgt tttaatactt
      841 ataataataa agaaatttat gatttgcagt ttatctaaaa attgatttaa gagggtagtt
      901 gtttattgcg aaaaatatca ttcaatttta atgaaataat ggcgtcatta ctataaaata
      961 ttactttatg ttgtaatgca tttttctata agatagaact aaaaggaggg gcaaagatgc
     1021 aaattagaca aatacatcaa catgactttg ctcaagttga ccagttaatt agaacggcat
     1081 ttgaaaatag tgaacatggt tatggtaatg aatcagagct agtagaccaa attcgtctaa
     1141 gtgatacgta tgacaataac ttagaaatag tagctgttct tcaaaacgaa gttgtagggc
     1201 acggtttact aagtgaagtt tatcttgata acggagcaca acgggaaatt ggattagtgt
     1261 tagcacctgt atctgttgat attcatcatc aaaataaagg tattgggaag cgattgattc
     1321 aagcattaga acgagaagca atattaaaag gatataattt tatcagtgta ttaggatggc
     1381 cgacgtatta tgccaatcta ggatatcaac gcgcaagtat gtacgacatt tatccaccat
     1441 atgatggtat accagacgaa gcgtttttaa ttaaagaatt aaaagtgaac agtttagcgg
     1501 gaaaaacagg taccataaat tacacatctg cttttgaaaa aatatgattt aaagctagga
     1561 ttacattagg tagagttcat attaataata aaaaatgttt gcaatcaaat cgtacgttgt
     1621 cgtttgtaat tcttaaaata gcaataaata aaatgtttgt tagtaaagta ttattgtgga
     1681 taataaaata tcgatacaaa ttaattgcta taatgcaatt ttagtgtata attccattaa
     1741 cagagattaa atatatcttt aaagggtata tagttaatat aaaatgactt tttaaaaaga
     1801 gggaataaaa tgaatatgaa gaaaaaagaa aaacacgcaa ttcggaaaaa atcgattggc
     1861 gtggcttcag tgcttgtagg tacgttaatc ggttttggac tactcagcag taaagaagca
     1921 gatgcaagtg aaaatagtgt tacgcaatct gatagcgcaa gtaacgaaag caaaagtaat
     1981 gattcaagta gcgttagtgc tgcacctaaa acagacgaca caaacgtgag tgatactaaa
     2041 acatcgtcaa acactaataa tggcgaaacg agtgtggcgc aaaatccagc acaacaggaa
     2101 acgacacaat catcatcaac aaatgcaact acggaagaaa cgccggtaac tggtgaagct
     2161 actactacga caacgaatca agctaataca ccggcaacaa ctcaatcaag caatacaaat
     2221 gcggaggaat tagtgaatca aacaagtaat gaaacgactt ctaatgatac taatacagta
     2281 tcatctgtaa attcacctca aaattctaca aatgcggaaa atgtttcaac aacgcaagat
     2341 acttcaactg aagcaacacc ttcaaacaat gaatcagctc cacagaatac agatgcaagt
     2401 aataaagatg tagttagtca agcggttaat ccaagtacgc ctagaatgag agcatttagt
     2461 ttagcggcag tagctgcaga tgcaccggca gctggcacag atattacgaa tcagttgaca
     2521 gatgtgaaag ttactattga ctctggtacg actgtgtatc cgcaccaagc aggttatgtc
     2581 aaactgaatt atggtttttc agtgcctaat tctgctgtta aaggtgacac attcaaaata
     2641 actgtaccta aagaattaaa cttaaatggt gtaacttcaa ctgctaaagt gccaccaatt
     2701 atggctggag atcaagtatt ggcaaatggt gtaatcgata gtgatggtaa tgttatttat
     2761 acatttacag actatgttga taataaagaa aatgtaacag ctaatattac tatgccagct
     2821 tatattgacc ctgaaaatgt tacaaagaca ggtaatgtga cattgacaac tggcatagga
     2881 accaatactg ctagtaagac agtattaatc gactatgaga aatatggaca attccataat
     2941 ttatcaatta aaggtacgat tgatcaaatc gataaaacaa ataatacgta tcgccaaaca
     3001 atttatgtca atccaagcgg agataacgtt gtgttacctg ccttaacagg taatttaatt
     3061 cctaatacaa agagtaatgc gttaatagat gcaaaaaaca ctgatattaa agtttataga
     3121 gtcgataatg ctaatgattt atctgaaagt tattatgtga atcctagcga ttttgaagat
     3181 gtaactaatc aagttagaat ttcatttcca aatgctaatc aatacaaagt agaatttcct
     3241 acggacgatg accaaattac aacaccgtat attgtagttg ttaatggcca tattgatcct
     3301 gctagtacag gtgatttagc actacgttcg acattttatg gttatgattc taattttata
     3361 tggagatcta tgtcatggga caacgaagta gcatttaata acggatcagg ttctggtgac
     3421 ggtatcgata aaccagttgt tcctgaacaa cctgatgagc ctggtgaaat tgaaccaatt
     3481 ccagaggatt cagattctga cccaggttca gattctggca gcgattctaa ttcagatagc
     3541 ggttcagatt ctggcagtga ttctacatca gatagtggtt cagattcagc gagtgattca
     3601 gattcagcaa gtgattcaga ctcagcgagt gattcagatt cagcaagtga ttcagattca
     3661 gcaagtgatt cagattcagc aagtgattca gactcagcaa gtgattcaga ttcagcaagt
     3721 gattcagatt cagcaagcga ttcagattca gcgagcgatt cagattcagc gagcgattca
     3781 gattcagcga gtgattccga ctcagcgagc gattcagact cagatagtga ctcagattcc
     3841 gatagcgatt ccgactcaga tagcgactca gattcagaca gcgattctga ctcagacagc
     3901 gattctgact cagacagtga ctcagattcc gatagcgatt ccgactcaga cagtgactca
     3961 gattccgata gcgattccga ctcagacagt gactcagatt ccgatagcga ttcagattcc
     4021 gacagtgatt ccgactcaga tagcgattcc gactcagata gcgactcaga ttcagacagc
     4081 gattcagatt cagacagcga ttctgactca gacagtgact cagattccga tagcgattca
     4141 gattcagaca gtgattcaga ctcagatagc gattcagatt ccgacagtga ctcagactca
     4201 gacagcgatt cagattccga tagcgattca gattccgaca gtgactcaga ttccgatagt
     4261 gactcggatt cagcgagtga ttcagattca gatagcgatt cagaatcaga tagtgactca
     4321 gactcagaca gtgattcaga ttcagatagt gactcagact cagacagcga ttcagaatca
     4381 gatagtgact ccgattcaga cagcgattca gaatcagata gtgactccga ttcagatagc
     4441 gattcggatt cagcgagtga ttcagactca ggtagtgact ccgattcatc aagtgattca
     4501 gattccgatt caacgagtga cacaggatca gacaacgact cagacagtga ttcaaatagc
     4561 gattccgagt caggttctaa caataatgta gttccgccta attcacctaa aaatggtact
     4621 aatgcttcta ataaaaatga ggctaaagat agtaaagaac cattaccaga tacaggttct
     4681 gaagatgaag cgaatacgtc actaatttgg ggattattag catcattagg ttcattacta
     4741 cttttcagaa gaaaaaaaga a
//
LOCUS       DANBWH010000191         4719 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.198, whole genome shotgun sequence.
ACCESSION   DANBWH010000191 DANBWH010000000
VERSION     DANBWH010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.198"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(34..303)
                     /gene="ffs"
                     /locus_tag="PPY92_002284"
     ncRNA           complement(34..303)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PPY92_002284"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(365..1093)
                     /gene="treR"
                     /locus_tag="PPY92_002285"
     CDS             complement(365..1093)
                     /gene="treR"
                     /locus_tag="PPY92_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HDJ2626262.1"
                     /translation="MAKQKKFMKIYEALKEDILNGQIQYGEQIPSEHDLVQLYQSSRE
                     TVRKALDLLALDGMIQKIHGKGSLVIYQEVTEFPFSELVSFKEMQEEMGVAYLTEVVV
                     NEVVEAHEVPEVQHALNINSSESLIHIVRTRRLNQHVKIVDEDYFLKSIVSDIGNDVA
                     SDSIYDYLEKVLNLNISYSSKSITFEPFDEQAYQLFGDVSVAYSATVRSIVYLENTMP
                     FQYNISKHLANEFKFNDFSRRRIK"
     gene            complement(1118..2758)
                     /gene="treC"
                     /locus_tag="PPY92_002286"
     CDS             complement(1118..2758)
                     /gene="treC"
                     /locus_tag="PPY92_002286"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDJ2626263.1"
                     /translation="MSKEIDWRKSVVYQIYPKSFNDTTGNGIGDINGIIEKLDYIKLL
                     GVDYIWLTPVYESPMNDNGYDISNYLEINEAFGTMDDFEKLIKVAHQKDLKVMLDIVI
                     NHTSTEHEWFKEARKSKDNPYRDYYFFRSSEDGPPTNWHSKFGGNAWKYDSETDEYYL
                     HLFDVSQADLNWDNPEVRQSLYRIVNHWIDFGVDGFRFDVINLISKGEFKDSDKIGKE
                     FYTDGPRVHEFLHELNRQTFGNTDMMTVGEMSSTTIENCIKYTQPERQELNSVFNFHH
                     LKVDYVDGEKWTNAKLDFHKLKEILMQWQRGIYDGGGWNAIFWCNHDQPRVVSRFGDD
                     TSEEMRIQSAKMLAIALHMLQGTPYIYQGEEIGMTDPHFTSIAQYRDVESINAYHQLL
                     SEGHAEADVLAILGQKSRDNSRTPMQWSDDVNAGFTAGKPWIDISENYHQVNVRQALQ
                     NKESIFYTYQKLIQLRHTHDIITYGDIVPRFMDHDHLFVYERHYKNQQWLVIANFSAS
                     AVDLPEGLAREGCVVIQTGTVENNTISGFGAIVIETNA"
     gene            complement(2822..4249)
                     /gene="treP"
                     /locus_tag="PPY92_002287"
     CDS             complement(2822..4249)
                     /gene="treP"
                     /locus_tag="PPY92_002287"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HDJ2626264.1"
                     /translation="MAVKREDVKAIVSAIGGKENLEAATHCVTRLRLVLKDESKVDKD
                     ALSNNALVKGQFKADHQYQIVIGPGTVDEVYKQFIDETGAQEASKDEAKQAAAKKGNP
                     VQRLIKLLGDIFIPILPAIVTAGLLMGINNLLTMKGLFGPKALIEMYPQIADISNIIN
                     VIASTAFIFLPALIGWSSMRVFGGSPILGIVLGLILMHPQLVSQYDLAKGNIPTWNLF
                     GLEIKQLNYQGQVLPVLIAAYVLAKIEKGLNKVVHDSIKMLVVGPVALLVTGFLAFII
                     IGPVALLIGTGITSGVTFIFQHAGWLGGAIYGLLYAPLVITGLHHMFLAVDFQLMGSS
                     LGGTYLWPIVAISNICQGSAAFGAWFVYKRRKMVKEEGLALTSCISAMLGVTEPAMFG
                     VNLPLKYPFIAAISTSCVLGAIVGMNNVLGKVGVGGVPAFISIQKEFWPVYLIVTAIA
                     IVVPCILTIVMSHFSKQKAKEIVED"
BASE COUNT         1546 a         1044 c          651 g         1478 t
ORIGIN      
        1 taataatcgg tttaatattt aaaaaataaa aaaccgtgca cctaagcatc gaacgtaatt
       61 cacgaacgta accaaagtcg ttagctaaat ctcggctacc ctacggcaca tatgatgatc
      121 cacttaatgc tgcttccgtc aggacctgac atggttcatg ggttcatatt gcataggacc
      181 gaaatcttca aacactacgt gctttgggca gacttcgcaa aaatacggcc tcaacaaagg
      241 aattaagcct cgcataaagc ggatttcgag tacagggaac cgctacctcc ccacctagca
      301 cggcaagata tatattacta tattttaata gttaattgca agtataaatc atttatatca
      361 ttgtttactt tatacgacgt cttgagaagt cgttaaattt aaattcattt gcaagatgtt
      421 ttgaaatatt atattgaaac ggcattgtat tttctaaata cacaatactt cgaactgttg
      481 ctgaataagc caccgataca tcaccaaaca attgatatgc ttgttcatca aacggttcaa
      541 aagtaataga cttacttgaa taactaatat taagatttaa taccttttcc aaataatcat
      601 aaatagaatc actcgcaaca tcattaccta tatctgaaac aatcgacttt agaaaataat
      661 cttcatcaac aatcttcaca tgttggttaa gccgacgagt tctaacaata tgaatgagcg
      721 attcactaga attgatgttt aaagcatgtt gaacttctgg aacttcatgc gcttcaacaa
      781 cctcattcac aacaacttca gttaaatatg cgacgcccat ttcttcttgc atttctttaa
      841 aactaacaag ttcagaaaat ggaaactctg taacctcctg ataaatgaca agtgaccctt
      901 taccatgaat cttttgaatc atgccgtcta atgccaacaa atctaatgcc ttacgcacgg
      961 tctctcgaga tgactggtac aattgcacca aatcatgttc agacggaatt tgttcaccat
     1021 attgaatctg cccgtttaat atatcttctt tcaacgcctc ataaatcttc ataaactttt
     1081 tttgtttcgc catataaacg catccactca atttatttta cgcgtttgtt tcgattacaa
     1141 ttgcaccaaa cccgcttatc gtattatttt ccactgtgcc tgtttgaatc acaacacaac
     1201 cttctctagc caatccttct ggcaaatcaa cagccgatgc tgagaaattc gcaattacta
     1261 gccattgttg attcttataa tgacgttcat aaacaaataa atgatcatga tccataaaac
     1321 gtggcacaat gtctccatac gtaataatat catgcgtatg tcttaattgt attaattttt
     1381 gatacgtata gaaaatagac tctttattct gaagtgcttg tctaacgttg acctgatgat
     1441 aattttccga aatatcaatc caaggcttac cagctgtaaa tccagcatta acatcatcac
     1501 tccattgcat aggcgttctc gaattgtctc gtgacttctg tcctaaaatc gctaatacat
     1561 ccgcttcagc atgcccttca cttagcaact gatggtaggc attaatcgat tcaacatcac
     1621 gatattgtgc tattgatgta aaatgtgggt ccgtcatacc aatttcttca ccttggtaaa
     1681 tatatggcgt cccttgcaac atatgcagtg cgatagctaa cattttagca ctttgtatcc
     1741 tcatctcttc cgacgtatca tcaccaaatc tagacactac ccgtggctga tcatgattac
     1801 accagaaaat cgcgttccat ccgccaccgt cataaatacc tcgttgccat tgcatcagaa
     1861 tttcctttaa cttatgaaaa tcaagcttcg catttgtcca cttttcacca tcaacataat
     1921 caacctttag atgatgaaaa ttaaaaacac tattcaattc ttggcgttct ggttgtgtat
     1981 acttaataca attttcaatc gtcgtcgaag acatttctcc tacagtcatc atgtcagtgt
     2041 taccaaacgt ttgacgattt aattcatgca gaaactcatg cactctagga ccatccgtat
     2101 aaaattcttt acctattttg tcagagtcct taaattcacc tttagaaatt aagttaatga
     2161 catcaaatcg aaaaccatca acgccgaagt ctatccaatg attgactatg cgatataacg
     2221 attgacgtac ttccggatta tcccaattta aatcagcttg actgacatca aataaatgta
     2281 aataatattc atctgtctca gaatcatact tccatgcatt accaccgaat ttagaatgcc
     2341 aatttgttgg cggcccgtct tcagatgatc tgaaaaagta ataatctcta taagggttat
     2401 ctttagattt acgggcttct ttaaaccatt catgctccgt cgacgtatga ttaataacaa
     2461 tatctaacat cactttcaag tctttttgat gagcaacttt gattaacttt tcaaaatcat
     2521 ccatcgttcc aaaggcttca ttgatttcta aataattgct gatatcatag ccattatcat
     2581 tcataggtga ttcatacact ggtgttaacc aaatataatc aacacccaat aacttgatat
     2641 aatccaattt ttctataatt ccgttgatat ctcctatacc attccccgtc gtatcattaa
     2701 acgacttagg ataaatttga tatacaacgg attttctcca atctatttct ttcgacacac
     2761 aattaccacc ttataacgta ataaaggacg tccaaataac gaacgcccct tttttatttt
     2821 attaatcttc aacaatttct ttcgcttttt gtttactaaa atgagacatc acaattgtta
     2881 gtatacatgg tacaacaata gcaatagctg tcacaataag atatactggc caaaattctt
     2941 tttgaattga aatgaatgct ggcacgccac caacaccaac ttttccaagt acgttattca
     3001 taccaacgat tgcccccaat acacaagacg ttgatatcgc agcgataaat ggatatttca
     3061 aaggtaagtt cacaccaaac atagctggtt cagtaacacc taacatagca gaaatacaag
     3121 atgttaatgc caagccttct tctttaacca ttttacgacg tttatagaca aaccatgctc
     3181 caaatgctgc agagccctga caaatattgg aaatcgcaac aattggccat aaatacgtac
     3241 cacctaagct gctacccatc aattggaaat ctactgctaa aaacatatgg tgtagtcctg
     3301 taattacaag tggtgcatat aacaatccat atattgctcc gccaagccat cctgcatgtt
     3361 ggaatataaa tgtaacacca gatgtaatac ctgtaccaat caataatgca actggtccaa
     3421 tgataataaa tgctaaaaat ccagtaacta aaagcgctac gggtccaacg accaacattt
     3481 ttatcgaatc gtgaacgact ttatttaatc ctttttcaat tttagctaga acataagctg
     3541 caattaaaac aggcaacact tgaccttggt aattcaactg cttaatctct aagccaaata
     3601 agttccacgt cggaatattc ccttttgcca aatcatactg agatactaat tgcggatgca
     3661 ttaaaatcaa acctaagact atgcctagaa tcggactacc accaaataca cgcatactac
     3721 tccaaccaat taatgctggt aagaaaataa atgccgtact cgcaatcaca ttaatgatgt
     3781 ttgaaatatc agcaatttgc ggatacatct caataagtgc ttttggacca aataaacctt
     3841 tcattgtaag taaattattg atccccatta acaaaccagc tgtcacaatc gcaggtaata
     3901 ttggtataaa aatatcccct aacaatttga tcaaacgttg tactggattc ccttttttcg
     3961 cagctgcttg tttcgcttca tctttcgaag cttcttgagc acctgtttca tcaataaact
     4021 gcttatacac ttcatcgact gttcctggac caatgacaat ttgatattga tggtctgctt
     4081 tgaactgccc cttgaccaac gcgttattac ttaatgcgtc tttatcaact ttgctttcat
     4141 ctttaagcac taaacgtaat cgtgttacac aatgcgttgc agcttcaaga ttttcttttc
     4201 ccccaatagc gcttacgatg gcttttacat cttctctttt tacagccata tatttcactc
     4261 ccttaatgtc ttaaaacgag tataacttgt ctagacaagt tatgtaaagg ctttcaacaa
     4321 attattttta ttttgtcaca tgatctgata cagctatttt agaaaatcaa taggccataa
     4381 cgacatatgt agcaataact cctcgctaca attgcgtgat taacaatacc tcaccacgct
     4441 ctcaccatta taaagtgtag atgcatggcc aactcctaaa ttgaaaaatg catatctata
     4501 aatagcaaac ttgcccacac aagccatcca tcatatttca caaaacaaaa aagacattta
     4561 gctatgtata ttgcacaagc caaatgccct tccaatctca atgccctatc atttctatag
     4621 ctaaaaaaac gagccattca aagacaaata aaaaaggcaa atactcaaaa tttgagcact
     4681 taccttcatt atatatagta tttaatataa atattgatg
//
LOCUS       DANBWH010000192         4642 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.199, whole genome shotgun sequence.
ACCESSION   DANBWH010000192 DANBWH010000000
VERSION     DANBWH010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.199"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(53..1372)
                     /locus_tag="PPY92_002288"
     CDS             complement(53..1372)
                     /locus_tag="PPY92_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1181 family transposase"
                     /protein_id="HDJ2626265.1"
                     /translation="MCNDTLELLRIKDENIKYINQEIDVIIKGKKATVVNAVLTYKPS
                     ACYCCGVKNEGQIHKHGKRVSRITLLKTQGYNTYLNLAKQRFKCLECNGTFTAKTSIV
                     DESCFISRCVTQKVIEEATKVKTEIDTAEDNCISPSTVSRIRTKAANSLRIKPFNCLP
                     EHIAMDEFKSVKNVTGSMSFIFIDNDTHDVIDILENRTTRFLRAYFERFDLKNRQQVK
                     TVTIDMYEPYVRLFRDLFPNAAIIFDRFHIVQHLNRELNKYRVQVMNEYRNKKGPNYT
                     IFKNNWKVLLMDTSKTIFSKYRWNKSFKAYKRSSDIVEFMLSKDDILRRSYELVQGLR
                     KDLRLCNWPKFINRLNSVSKKSVSKGVWKVVKYYRKHQRMLRNTIYYPAFNNGAIEGI
                     NNKIKLIKRISFGYRNFNNFKARIMMIFSLYKGEKKKTTKPNNGLAA"
     gene            complement(1581..4466)
                     /gene="fnbB"
                     /locus_tag="PPY92_002289"
     CDS             complement(1581..4466)
                     /gene="fnbB"
                     /locus_tag="PPY92_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbB"
                     /protein_id="HDJ2626266.1"
                     /translation="MKSNLRYGIRKHKLGAASVFLGTMIVVGMGQEKEAAASEQNNTT
                     VEESGSSATESKASETQTTTNNVNTIDETQSYSATSTEQPSKSTQVTTEEAPTTVQAP
                     KVETEMKSQEDLPSEKVADKETTGTQVDIAQPSNVSEIKPRMKRSADVTAVSEKEVAE
                     EAKATGTDVTNKVEVTESSLEGHNKDSNIVNPHNAQRVTLKYKWKFGEGIKAGDYFDF
                     TLSDNVETHGISTLRKVPEIKSSTEDKVMANGQVINERTIRYTFTDYINNKKDLTAEL
                     NLNLFIDPTTVTKQGSQKVEVTLGQNKVSKEFDIKYLDGVKDRMGVTVNGRIDTLNKE
                     EGKFSHFAYVKPNNQSLTSVTVTGQVTSGYKQSANNPTVKVYKHIGSDELAESVYAKL
                     DDTSKFEDVTEKVNLSYTSNGGYTLNLGDLDNSKDYVIKYEGEYDQNAKDLNFRTHLS
                     GYHKYYPYYPYYPYYPVQLTWNNGVAFYSNNAKGDGKDKPNDPIIEKSEPIDLDIKSE
                     PPVEKHELTGTIEESNDSKPIDFEYHTAVEGAEGHAEGIIETEEDSIHVDFEESTHEN
                     SKHHADVVEYEEDTNPGGGQVTTESNLVEFDEESTKGIVTGAVSDHTTVEDTKEYTTE
                     SNLIELVDELPEEHGQAQGPIEEITENNHHISHSGLGTENGHGNYGVIDEIEENSHVD
                     IKSELGYEGGQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNNGNQSFEE
                     DTEEDKPKYEQGGNIIDIDFDSVPQIHGFNKHNEIIEEDTNKDKPNYQFGGHNSVDFE
                     EDTLPKVSGQNEGQQTIEEDTTPPTPPTPEVPSEPETPTPPTPEVPSEPGEPTPPKPE
                     VPSEPETPVPPTPEVPSEPGKPVPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVAP
                     TKQKQSKKSELPETGGEESTNKGMLFGGLFSILGLVLLRRNKKNNKA"
BASE COUNT         1232 a          840 c          722 g         1848 t
ORIGIN      
        1 ggtatataat ttaaagaact attttaaatt acaactttta gagtttttat tattaggcgg
       61 ccagtccatt attgggcttg gttgtcttct ttttttctcc tttgtataag ctgaaaatca
      121 tcattatacg tgctttaaag ttgttgaaat ttctgtaacc aaaagaaatt cgcttgatta
      181 attttatctt attattaatt ccttctatag caccattatt aaatgctggg taataaattg
      241 tatttcttaa catcctttga tgttttctat aatatttaac cactttccat acacctttac
      301 tcacagactt tttactaact gaatttaaac gattaataaa tttaggccaa ttacataacc
      361 ttagatcttt tcttaatcct tggacaagtt cgtaggagcg tcgtagtata tcgtcttttg
      421 aaagcatgaa ttctacaatg tcagatgagc gtttataagc cttaaaagat ttattccatc
      481 tgtatttact aaatatggtt ttactagtat ccatcaatag aactttccag ttattcttaa
      541 aaattgtata attaggtcct tttttattac ggtattcatt cataacttgt acacgatact
      601 tattaagttc cctatttaaa tgttgaacga tatggaatct gtcaaaaata atagctgcat
      661 taggaaatag gtcgcgaaat aatcggacat agggttcata catgtcaata gtaaccgtct
      721 taacttgttg tcgatttttt aaatcgaatc gctcgaaata ggcacgcaag aatcttgtag
      781 ttctattttc taaaatatct ataacatcat gagtatcatt atctataaaa atgaaactca
      841 ttgatccagt tacattttta acgcttttaa attcatccat agcgatgtgt tctggcaaac
      901 aattaaacgg tttaattcgt aatgaattag ccgctttagt tctaatacga cttacagtag
      961 atggagagat acagttatct tctgcagtat caatctctgt tttaacttta gtagcttctt
     1021 ctataacttt ttgagtaaca catcttgaga taaaacacga ctcatcaaca attgacgttt
     1081 tagcagtaaa agtgccattg cattctaagc atttaaaacg ttgtttagct aagttgaggt
     1141 atgtgttata cccttgagtt ttaagtaaag taatacgaga aacacgctta ccatgtttat
     1201 gaatttgtcc ttcattttta actccacaac aataacaggc cgaaggctta tacgttagta
     1261 cagcattaac cactgttgct ttttttcctt tgataatgac gtcaatttct tggtttatat
     1321 attttatatt ttcatctttt attcttagta attctaaggt atcattacac ataggcgcac
     1381 ttgtctcctt tagtttgggt ttggtcactt taaattatag aggcaattgc gctttttgtg
     1441 tattaaaaaa agcaggaagt tttaccttcc caccataaaa tatgaagaac ctaattattt
     1501 cttgggaaat ttgatttaat tttaaaaaaa caggtttact tcatataatt tatgaaataa
     1561 acccgtcaat ttttgtttaa ttatgctttg ttattctttt tatttctgcg taataatact
     1621 aaacctagaa tgctgaataa tccgccgaac aacatacctt tgtttgttga ttcttctcca
     1681 cctgtttcag gtagttcaga tttcttagat tgtttttgtt tagttggtgc cactgcttta
     1741 accttttcat tgatttcaat aacaggtgtt actaccttac cttgttccac tggtttagaa
     1801 ggttttttag gttcttcttt agcaggtggt actggtttac caggttcaga tggtacctct
     1861 ggtgttggtg gtactggtgt ttccggctca cttggtactt ccggttttgg tggcgttggt
     1921 tcgcctggct cactcggtac ttctggtgtt ggtggtgttg gtgtttccgg ctcacttggt
     1981 acctctggtg ttggcggtgt tggcggtgtt gtatcttctt caatcgtttg ttgaccttca
     2041 ttttgaccac ttacttttgg aagtgtatct tcttcaaaat caacactgtt gtgtccacca
     2101 aattgataat taggtttatc tttgtttgta tcttcttcaa taatttcatt atgcttattg
     2161 aatccatgaa tttgtggcac actgtcgaag tcgatatcaa tgatgttacc accttgttca
     2221 tacttaggct tgtcttcttc tgtgtcttcc tcgaatgact ggttaccatt attttgacca
     2281 tgaatttgag gtacactgtc gaaatcgata tctacgatat taccaccttg ttcatattta
     2341 ggtttatctt cttctgtgtc ttcctcgaat gactgattac cgctattttg gccaccttca
     2401 taacctaatt cactcttaat atcaacgtgg ctattttctt cgatttcatc aatcacgcca
     2461 taattaccgt gaccattttc agttcctaaa ccagaatgag aaatatgatg attgttttca
     2521 gtaatttcct cgattggccc ttgtgcttga ccatgttctt caggtaattc atccactaat
     2581 tcaatcagat tactttcagt tgtatattct ttcgtatctt caactgttgt atggtcgctc
     2641 actgcgccag ttacaatacc ttttgtagac tcttcgtcaa attcaactaa gttagactca
     2701 gttgttactt ggccaccacc tgggtttgta tcctcttcat attcaacaac atcagcgtga
     2761 tgttttgaat tttcatgtgt agattcttca aaatccacat gaatagaatc ttcttcagtt
     2821 tcaataatac cttctgcatg accttctgca ccttcaacag ctgtatgata ttcaaaatca
     2881 attggcttag aatcgttact ttcttcgatt gtaccagtca attcatgctt ctccactggt
     2941 ggctctgatt taatgtctaa atcaattggt tcactcttct caatgatagg atcatttggt
     3001 ttatctttac catcgccttt agcattatta gagtaaaatg caacaccgtt gttccaagtt
     3061 aattgaactg gataatacgg gtaataagga tagtatgggt agtatttatg atatcctgaa
     3121 agatgtgttc ggaaatttag atccttagca ttttgatcat attcaccttc atatttaatt
     3181 acatagtctt tcgaattatc taaatcgcca aggttcaatg tgtacccacc attacttgtg
     3241 taagatagat ttactttttc agtcacatct tcaaatttac tggtatcatc aagctttgca
     3301 taaacacttt cagctaattc atctgaacca atgtgtttat atactttgac tgttggatta
     3361 ttagcacttt gtttatatcc agatgttact tgaccagtta ctgtgacaga agttaacgac
     3421 tggttgttag gcttcacata tgcaaaatgg ctaaatttac cctcttcttt attcaaagta
     3481 tcaatacgac cattaacagt aacacccatt ctatctttaa cgccgtctaa atatttgata
     3541 tcaaattctt ttgagacttt attttgacct agtgttactt caactttttg actcccttgc
     3601 tttgtcactg ttgttgggtc aatgaatagg tttaagttta attcagcagt taaatctttt
     3661 ttgttattta tataatcagt aaatgtatag cgaattgtac gttcatttat aacttgacca
     3721 tttgccataa ctttatcttc tgttgaactt tttatctccg gaactttacg cagtgttgat
     3781 ataccatgtg tttcaacatt atcacttaat gtgaaatcaa aataatctcc tgccttaatt
     3841 ccttctccaa atttccattt gtattttaaa gttactcttt gagcattatg cggattaaca
     3901 atattcgaat ctttattatg tccttctaaa gagctttcag taacttccac tttatttgtt
     3961 acatctgtac ctgtcgcttt agcttcttcc gctacttctt tctctgaaac tgctgtaacg
     4021 tcagctgatc ttttcattct tggtttaatt tctgagacgt tacttggttg agctatgtca
     4081 acttgagttc ctgtagtttc cttatcagca actttttctg atggtaaatc ttcttgtgat
     4141 ttcatttcgg tttctacttt tggtgcttgc acagttgttg gtgcttcttc tgttgttact
     4201 tgagttgatt ttgatggttg ctcagttgat gtcgcgctgt atgattgtgt ttcatctatt
     4261 gtattaacgt tatttgtagt tgtttgtgtt tcgcttgctt tactttcagt agctgaactc
     4321 ccactttcct ctactgtagt attgttttgt tccgatgctg cagcttcttt ttcttgtccc
     4381 attccaacaa cgatcattgt tcctaagaat actgaggccg ctcccaattt gtgttttctt
     4441 atgccgtatc taagattgct tttcactata atattctccc ttaaatgcaa aattcattta
     4501 tttttaaaac tcaataaatg caattctata ttgttcggtt tttaaaagca atgaaaaaaa
     4561 gcgagttaat aaaaagttaa gattgttgtt aactttatgt ataatgagtt ttttattatt
     4621 tataactcac atatatattg ca
//
LOCUS       DANBWH010000193         4619 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.201, whole genome shotgun sequence.
ACCESSION   DANBWH010000193 DANBWH010000000
VERSION     DANBWH010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.201"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..276
                     /locus_tag="PPY92_002290"
     CDS             <1..276
                     /locus_tag="PPY92_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HDJ2626267.1"
                     /translation="MLYFILKDENIVEIQLLVTMSYQYILGVLLGEVLQSSWTIAKLT
                     FQRPSPQWDGSALKELSHVPIFIYSTIWIIFNLYAVNILLKYTHLIN"
     gene            complement(356..1000)
                     /locus_tag="PPY92_002291"
     CDS             complement(356..1000)
                     /locus_tag="PPY92_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HDJ2626268.1"
                     /translation="MLTEEKEDYLKAILTNNGDKNFVTNKILSQFLNIKPPSVSEMVG
                     RLEKAGYVETKPYKGVRLTEDGLTHTLDIIKRHRLLELFLIEILKYNWEEVHQEAEIL
                     EHRISDLFVERLDSLLNFPETCPHGGVIPRNNEYKEKYITTILNYEPGDIVTIKRVRD
                     KTDLLIYLSSKDISIGNEVEIVSKDEMNKVIIIKRNDNVIIVSYENAMNMFAEK"
     gene            1122..1865
                     /locus_tag="PPY92_002292"
     CDS             1122..1865
                     /locus_tag="PPY92_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDJ2626269.1"
                     /translation="MLETKDLNLFLGNKHVLKNISLSIPVRGEIIGIMGPNGAGKSSL
                     IKSLIGEFNATGTKLLYNKPIQQQLQHITYIPQKAHIDLDFPISVEQVILSGCYKEIG
                     WFRRPNKSARDKLKQLLSDLELESLRHRQISELSGGQLQRVLVARALMSESEVYFLDE
                     PFVGIDFSSEKLIMTKIENLKQQGKLILIIHHDLSKAKQYFDRIILLNQTLRYFGDSE
                     EAMSVTRLNETFMSSTDCSDPSQRSNITC"
     gene            1859..2695
                     /locus_tag="PPY92_002293"
     CDS             1859..2695
                     /locus_tag="PPY92_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDJ2626270.1"
                     /translation="MLEFVEHLFTYQFLNRALITSIIVGIVCGTVGSLIVLRGLSLMG
                     DAMSHAVLPGVALSFLFGIPMFVGALITGMIASIFIGYITSSSKTKPDAAIGISFTAF
                     LASGIIIISLINTTTDLYHILFGNLLAITNSAFLTTIVIGSIVLILIIIFYRPLMIST
                     FDPTFSRMSGLNTTLLHYFVMLLLSLVTVASIQTVGIILVVALLITPASTAFLISKKL
                     YSMMIIASLISVISSIVGLYYSYIYNIPSGATIVLCTFVIYIITLFFTKFTNRKKRGS
                     LT"
     gene            2692..3621
                     /locus_tag="PPY92_002294"
     CDS             2692..3621
                     /locus_tag="PPY92_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDJ2626271.1"
                     /translation="MKKLVPLLLALLLLVAACGTGGKQSSDKSNGKLKVVTTNSILYD
                     MAKNVGGDNVDIHSIVPVGQDPHEYEVKPKDIKKLTDADVILYNGLNLETGNGWFEKA
                     LEQAGKSLKDKKVIAVSKDVKPIYLNGEEGNKDKQDPHAWLSLDNGIKYVKTIQQTFI
                     DNDKKHKADYEKQGNKYIAQLEKLNNDSKDKFNDIPKEQRAMITSEGAFKYFSKQYGI
                     TPGYIWEINTEKQGTPEQMRQAIEFVKKHKLKHLLVETSVDKKAMESLSEETKKDIFG
                     EVYTDSIGKEGTKGDSYYKMMKSNIETVHGSMK"
BASE COUNT         1585 a          751 c          721 g         1562 t
ORIGIN      
        1 atgttgtatt ttatattaaa agatgaaaat atagtagaaa ttcaactact tgtcacaatg
       61 agttatcaat atattttagg tgtattatta ggcgaagttc tacaatctag ttggacgatt
      121 gctaaattga ctttccagcg tccgagtcca cagtgggatg gtagtgcact taaagaactc
      181 agtcatgtac ctatcttcat ttatagtaca atctggatta tcttcaatct ttatgcagtg
      241 aatattttac tcaaatatac tcacttgata aattaactaa taaaaaagaa gcctaaagca
      301 attatccata tgtctttatc agttcaatca tggatatctt tatggcttct ttattttatt
      361 tttcagcaaa catgttcatt gcattttcgt aactgacaat aattacatta tcattacgtt
      421 taatgataat tactttattc atttcatctt tcgatacaat ttccacttca ttaccaatag
      481 aaatatcttt actagacaaa tatattagca aatcggtctt atctctcaca cgtttgattg
      541 taacgatatc accaggttca taattcaaaa tcgttgttat atatttctct ttatattcat
      601 tatttctagg aatcacaccg ccgtgcgggc aagtttctgg gaaatttaac aggctatcca
      661 gcctttcaac aaataaatct gaaattcgat gttctaaaat ttctgcttct tgatgtactt
      721 cttcccaatt atatttcaat atttctatta aaaataattc taatagtcga tgtctcttaa
      781 tgatatcaag cgtatgcgtt aaaccatcct ctgttaatct aacacctttg tatggttttg
      841 tttcaacata gcctgctttt tcaagacgtc ctaccatttc acttacagat ggaggcttaa
      901 tatttaaaaa ttgagataag attttatttg tcacaaagtt tttatcgcca ttattcgtaa
      961 ggattgcctt taaatagtcc tctttttctt cagttaacat actttcacct cacatacatt
     1021 gtctatatta tatcacgaat ttacttgaca tgataaatat tctcagttta ttatacaatt
     1081 aattaggtta gcctaaactt ttaattagga ggtataaacg tttgttagaa acaaaagatt
     1141 taaatctgtt tttaggtaat aagcatgtac ttaaaaacat ttccttatcg ataccagtac
     1201 gcggcgaaat aattggtatc atgggcccga atggtgctgg taaatcttcc cttatcaagt
     1261 ctttaattgg tgaatttaat gctaccggta ctaaattgtt atataacaaa cctatacaac
     1321 aacaactgca acatattaca tatattccac aaaaagcaca tattgattta gattttccta
     1381 taagtgtgga acaagtgatt ttatcaggtt gctacaaaga aattggatgg tttagacgac
     1441 ctaataaatc agcaagggat aaactcaaac agttattaag cgatttagaa ttagaatctt
     1501 tacgtcatcg acaaatttca gaattaagtg gtggacaatt acaacgtgtg ctagtagcaa
     1561 gagcattgat gtccgaaagt gaagtttatt ttctagatga gccgtttgtc ggaattgatt
     1621 ttagtagcga aaaattaatc atgacaaaaa tcgagaactt aaaacaacaa ggaaaactta
     1681 ttcttatcat ccaccatgat ctatcaaaag caaagcaata ctttgatcgc attattctat
     1741 taaatcaaac attacgatac tttggtgata gtgaagaggc tatgagtgtc actcgcttaa
     1801 acgaaacatt tatgagtagc actgactgta gtgaccctag tcaaaggagc aatataacat
     1861 gttagagttt gtcgaacatt tatttacata tcaattcttg aatcgagcat tgataacttc
     1921 aattattgta gggatagttt gtggtacagt tggtagttta attgtattac gtggtctttc
     1981 attaatggga gatgcaatga gtcacgcagt attacctggt gttgccctat cattcttatt
     2041 tggtattcca atgtttgtag gcgcacttat aactggtatg atcgcaagta tttttatcgg
     2101 ttatatcaca tctagtagta aaacgaaacc tgacgccgct attggaatta gttttaccgc
     2161 tttcttagct tcggggatta ttattattag cttaataaac actacgacag atttgtacca
     2221 tattctgttt ggtaatttac tggcaatcac aaatagtgca tttttaacaa ctattgtgat
     2281 tggttcaatc gtccttattc taatcattat tttctatcgt ccattaatga tttctacatt
     2341 cgatccaacg tttagtagaa tgagtggtct aaatacgacg ttattacatt actttgtgat
     2401 gttgttactc tcattagtaa cagtagcaag tattcaaacg gtaggtatta ttcttgtagt
     2461 tgcattatta attacacctg catctacagc atttttaatt agtaaaaaac tatactcaat
     2521 gatgattata gcaagtttaa taagtgtcat aagttcaatt gtcggacttt attacagcta
     2581 tatttacaac attcctagtg gtgcgacaat cgtactttgt acgtttgtaa tttatatcat
     2641 cacattattt ttcacaaaat ttacgaatag aaagaaacga ggaagtttaa catgaaaaaa
     2701 ttagtacctt tattattagc cttattactt ctagttgctg catgtggtac tggtggtaaa
     2761 caaagcagtg ataagtcaaa tggcaaatta aaagtagtaa cgacgaattc aattttatat
     2821 gatatggcta aaaatgttgg tggagacaac gtcgatattc atagtattgt acctgttggt
     2881 caagatcctc atgaatatga agttaaacct aaagatatta aaaagttaac tgacgctgac
     2941 gttattttat acaacggatt aaatttagag actggtaacg gttggtttga aaaagcctta
     3001 gaacaggctg gtaaatcatt aaaagataaa aaagttatcg cagtatcaaa agatgttaaa
     3061 cctatctatt taaacggtga agaaggcaac aaagataaac aagatccaca cgcatggtta
     3121 agtttagata atggtattaa atacgtaaaa acaattcaac aaacatttat cgataacgac
     3181 aaaaaacata aagcagatta tgaaaagcaa ggtaacaaat acattgctca attggaaaaa
     3241 ttaaataatg acagtaaaga caaatttaat gacattccaa aagaacaacg tgccatgatt
     3301 acaagtgaag gtgccttcaa gtacttctca aaacaatacg gtattacacc aggttatatt
     3361 tgggaaatta acactgaaaa acaaggtaca cctgaacaaa tgagacaagc tattgagttt
     3421 gttaaaaagc acaaattaaa acacttatta gtagaaacaa gtgttgataa gaaagcaatg
     3481 gaaagtttat ctgaagaaac gaagaaagat atctttggtg aagtgtacac agattcaatc
     3541 ggtaaagaag gcactaaagg tgactcttac tacaaaatga tgaaatcaaa tattgaaact
     3601 gtacacggaa gcatgaaata acacgctgtg ttttaatgaa gtaagatgaa ttgatgttga
     3661 tgcaacctaa aatattggta tctccaatat tttaggctac acatcaacat aacaaagtcg
     3721 aaggctaata gtcccatatc gtgcgttaaa tatatattac cctcctatta atatatatac
     3781 cgttcccgat cgcacgatat ggtggtatta gaacttctct ttgaacgaaa gagaaaagct
     3841 agaagttctt atgcagtttt aattaaactg taaacatttg tcactcttta aatcaaagag
     3901 taaagttaaa agctttatgt ggttttgatt aaactgcgaa cagctgcttc tctttgaacg
     3961 aaagagaaaa gctagaagtt cttatgcagt tttaattaaa ctgtcgttcc cttcatctct
     4021 tttaaccaca gagatgcgtt agaagttctt ctaatacaat ttatacaacg acattcccta
     4081 cacactctta taaaagagat tcacgcgcgt caataaattg tattacatac taactaaaaa
     4141 gcttttctta atcgtactaa cgaagttaga ggttcttatg tgattggaca actgttctcc
     4201 ctactagact tttagcatag ctggttaaaa gtactattac agattgttca attgtatgcc
     4261 cctttccttt gaacggaata ttaaaagcta tgtgcagtaa cttttaatag gcataccact
     4321 tttgcatcaa gctataaata atcgcatact ttgatattta atctggccta tcaaagtaaa
     4381 catctatggt ttcctccccc catagatcct taagccacac tcatccgatg tcatatgagt
     4441 gtggctattt tttgtgaatg cacttatctt catctttaaa tgacttcgat taatctatct
     4501 cctccaattt ttaaaactta tttcaattaa atctcaaata ttaataattc gtatagttta
     4561 aaactatcta ttcaattccc ctaaaataaa agggattgat gaaaagccct tcagttttt
//
LOCUS       DANBWH010000194         4592 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.202, whole genome shotgun sequence.
ACCESSION   DANBWH010000194 DANBWH010000000
VERSION     DANBWH010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.202"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            40..1386
                     /locus_tag="PPY92_002295"
     CDS             40..1386
                     /locus_tag="PPY92_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDJ2626272.1"
                     /translation="MAKHPFEQFNLESSLIDAVKDLNFEKPTEIQNRIIPRILKRTNL
                     IGQSQTGTGKSHAFLLPLMQLIDSEIKEPQAIVVAPTRELAQQLYDAANHLSQFKAGV
                     SVKVFIGGTDIEKDRQRCNAQPQLIIGTPTRINDLAKTGHLHVHLASYLVIDEADLMI
                     DLGLIEDVDYIAARLEDNANIAVFSATIPQQLQPFLNKYLSHPEYVAVDSKKQNKKNI
                     EFFLIPTKGAAKVEKTLNLIDILNPYLCIIFCNSRDNANDLARSLNEAGIKVGMIHGG
                     LTPRERKQQMKRIRNLEFQYVIASDLASRGIDIEGVSHVINFDVPNDIDFFTHRVGRT
                     GRGNYKGVAITLYSPDEEHNISLIEDRGFVFNTVDIKDGELKEVKAHNQRQARMRKDD
                     HLTNQVKDKVRSKIKNKVKPGYKKKFKQEVEKMKRQERKQFSKQQNRQKRKQNKKG"
     gene            1396..2286
                     /locus_tag="PPY92_002296"
     CDS             1396..2286
                     /locus_tag="PPY92_002296"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDJ2626273.1"
                     /translation="MLLGSHVSMSGKKMLEGSAIEAYEYGETTFMIYTGAPQNTRRKS
                     IEDLNITKGHEVMEKYGLSNIVVHAPYIINIANTTKPETFNLGVDFLQQEIERTQAIG
                     AKDIVLHPGAHVGAGVDAGINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEEL
                     ARIIDGVHNNERLSVCFDTCHTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSK
                     NDRGAQKDRHENIGFGYIGFDALNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLE
                     IEMLKQQQFDPELKNKVMQQ"
     gene            2412..3197
                     /locus_tag="PPY92_002297"
     CDS             2412..3197
                     /locus_tag="PPY92_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDJ2626274.1"
                     /translation="MRGENTMTPVFELKNVNYYYDHKKVLENINIKINKGEFLAIVGP
                     NGAGKSTLLKLILGLLPLQSGEIFVEGIDFKNKKTSIKLSYVSQKANAFNSGFPASVK
                     EVVLSGLTKTKRLFQTFNSKDNEKVIKVLERLNISDLIHKNIAELSGGQQQRVMIARA
                     LISEPAVLVLDEPTNGIDAKHVSEFYNTLDQLKQEGITIILVTHDIGVVADTATEVAC
                     LNKHLHFHGTTDEFKSLDEVEISKIYGHPVRFVDHQHNRECCN"
     gene            3239..4102
                     /locus_tag="PPY92_002298"
     CDS             3239..4102
                     /locus_tag="PPY92_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDJ2626275.1"
                     /translation="MIDALLNFDFMRYSLISGILIGFIAPLIGAFIVVRRLSLIADAL
                     SHVTLGGISFGMFLLTIMPTLVFINPMWFGILFAIVGALLIEKLRTSYTAYQEIAIPI
                     IMSAGIALSAIFISLADGFNQEIVGLLFGSISAVNISDLTTIIVIAIIVVLFITLFYK
                     ELFILSFDEEYSKVIGIHKWIQFLFIVIVAMVISASMRVVGILLVSALITLPIAISMR
                     ITKSFKQLILLSVFLGELSVILGLVLAFYMDISPGGVIVVLLVILLMITMAYQKMRMK
                     FKKGANINEYK"
     gene            4089..4499
                     /locus_tag="PPY92_002299"
     CDS             4089..4499
                     /locus_tag="PPY92_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDJ2626276.1"
                     /translation="MNTNDAIKILKENGLKYTDKRKDMLDIFVEEDKYINAKYIQQVM
                     DENYPGISFDTIYRNLHLFKDLGIIENTELDGEMKFRIACTNHHHHHFICEKCGDTKV
                     IDYCPIDQIKLSLPGVNIHKHKLEVYGVCESCQD"
BASE COUNT         1671 a          601 c          848 g         1472 t
ORIGIN      
        1 tttataatag taaagatgaa catataagga ggccaaatca tggcaaaaca tccattcgaa
       61 caatttaatc tagaatctag tttaattgac gctgtgaaag accttaattt tgaaaaacca
      121 actgaaattc agaatcgaat tattccaaga atactaaaga gaacaaattt aattggtcaa
      181 tctcaaacgg gtacagggaa atctcatgca tttttattac cattaatgca gttaattgat
      241 agtgaaataa aagaaccaca agcaatcgta gttgcaccaa caagagaact tgcacaacaa
      301 ctatacgatg cagcgaacca tttaagccaa tttaaagctg gtgtttcagt taaagttttt
      361 attggtggta cagatataga gaaagataga caacgttgta atgcacaacc acaattgatt
      421 ataggcaccc ctactagaat taatgactta gctaaaacgg gacatttaca tgtgcactta
      481 gcatcatatt tagttattga tgaagcggat cttatgattg acttaggatt aattgaagat
      541 gtagattaca ttgctgcaag attggaagat aatgcaaata ttgcggtgtt tagtgctaca
      601 attccacaac agttacaacc atttttaaat aaatatttaa gtcatccaga atatgtagct
      661 gtcgacagta aaaaacaaaa taaaaagaac atcgaattct ttttaatacc tactaaaggt
      721 gcagctaaag ttgaaaaaac tttaaattta attgatatac taaatccata cttatgtatt
      781 attttctgta atagtagaga taatgcaaat gatttagcac gttcactaaa tgaagctggt
      841 attaaagttg gtatgattca tggtggttta acgccacgtg aacgtaaaca acaaatgaaa
      901 cgtatacgta atttagaatt ccaatacgtt attgccagcg atttagcatc tcgtggtatt
      961 gatattgaag gtgttagtca tgtcatcaat tttgatgtgc caaatgatat tgacttcttt
     1021 acgcatagag ttggacgaac tggtcgtggg aattataaag gtgtagcaat tacgctttat
     1081 agtcctgatg aagaacacaa tatttcatta atagaagatc gcggttttgt attcaatact
     1141 gttgatatta aagatggtga gttaaaagaa gttaaagcgc acaatcagcg tcaagcaaga
     1201 atgcgcaaag atgaccattt aactaatcaa gtgaaggaca aagttcgaag taaaattaaa
     1261 aacaaagtta aaccaggtta taagaagaaa tttaaacaag aagttgaaaa aatgaaacgt
     1321 caagagcgta aacaatttag taagcagcaa aatagacaaa aacgtaagca aaacaaaaaa
     1381 ggttaggtgt gttttatgtt attaggatca catgtttcaa tgagtggtaa aaagatgtta
     1441 gaaggttctg ctatagaagc gtatgaatat ggtgaaacaa catttatgat ttatactggt
     1501 gcacctcaaa acacgcgccg taaaagtatt gaagatttaa atataactaa aggtcatgaa
     1561 gtgatggaaa aatatggctt atctaatatt gttgttcatg caccatacat cattaatatt
     1621 gcaaatacaa ccaaaccaga aacgtttaat cttggtgtag atttcttgca acaagaaatt
     1681 gaaagaacgc aagctatagg cgctaaagat attgtattac atccaggtgc gcatgttgga
     1741 gctggtgttg atgccggaat taataagatt attgaaggat taaatgaagt tttaacgaat
     1801 gacaataatg tacgtattgc gcttgaaacg atggcaggta aaggtacaga gattggtcgc
     1861 tcgtttgaag agttagcacg tattattgat ggagttcata ataacgaacg tttatcagta
     1921 tgttttgata catgtcatac gcatgatgct ggatataatg tgaaagagga ctttgatggc
     1981 gtattaaatg aatttgataa aattattgga gtcgacagaa tcaaagttgt tcatgtcaat
     2041 gattctaaaa atgaccgtgg cgctcaaaaa gaccgacatg aaaatattgg cttcggttac
     2101 attggttttg atgcattaaa ttacattgtg catcatgata gttttaaaga tattccgaaa
     2161 atcttagaaa caccttatgt tggtgaagat aagaaaaata aaaaaccgcc atataaactt
     2221 gaaattgaaa tgttaaaaca acagcaattt gacccagaat taaaaaataa ggttatgcaa
     2281 caataaaatg ataatgctct cttcaagaaa tgtgataaat aaaacttgaa gggggcatat
     2341 ctctttattt tgtctaattt tgagtcgtaa acattactgt ttacatatgt tactaggagt
     2401 tgtatagttg aatgcgaggt gagaatacga tgacaccagt ctttgaattg aaaaatgtca
     2461 attactacta tgatcataaa aaagtgttag aaaatataaa cattaaaata aataaaggtg
     2521 aatttttagc gattgttgga ccaaatggtg ctggtaaatc aacattattg aagttgattc
     2581 tagggttatt acctttacaa agtggtgaga tttttgttga aggtattgat tttaaaaata
     2641 agaaaacatc aattaaatta agctatgtat cacaaaaagc aaatgccttt aattcaggtt
     2701 tcccagcaag tgttaaagaa gttgttttaa gcggattaac aaagacaaaa cgtcttttcc
     2761 aaacatttaa tagcaaagat aatgaaaaag tgattaaagt actagaaaga ctgaatataa
     2821 gtgatttaat tcataaaaat atagcagaat tatcaggtgg tcaacaacaa cgtgtaatga
     2881 ttgcccgagc attgatttca gaacctgcag tattagtact tgatgaacca acgaatggta
     2941 ttgatgcaaa acatgtaagt gaattttata atactttaga tcaattaaaa caagaaggta
     3001 tcaccattat cttagttact catgatatcg gtgttgtagc agatactgct actgaagtag
     3061 catgtttaaa taagcatttg catttccatg gtacaactga tgagtttaaa tcacttgatg
     3121 aagttgaaat ttcaaaaatt tatggacatc ctgtacgttt tgtcgatcat cagcataatc
     3181 gagaatgttg taattaataa agatttgagt ttatttaaaa tggaaaggcg tgtcacaatt
     3241 gatagatgca ttattgaatt ttgattttat gaggtactct ttaattagtg gtatcttgat
     3301 aggttttatt gcgcctctaa tcggtgcttt tatcgttgtt agacgactat cacttatagc
     3361 tgatgctcta agtcatgtaa ctttaggtgg tatatccttc ggtatgtttt tacttactat
     3421 tatgccaaca ctagtattta ttaatccaat gtggtttgga atcttattcg caatagtagg
     3481 tgcgcttcta attgaaaaat taagaacgtc atacactgct taccaagaaa ttgctattcc
     3541 aattataatg agtgctggta tcgccttgag tgcaatcttc atttcattag ctgatgggtt
     3601 caatcaagaa attgttggat tgctatttgg ctcaataagc gcagtgaata taagtgattt
     3661 aacaacgatt attgtcattg ccatcatagt tgttctgttt attacattgt tttataaaga
     3721 attatttata ttgtcatttg atgaggaata tagtaaagtc attggcattc ataaatggat
     3781 tcaattttta tttattgtta ttgtagcgat ggttatttct gcctcaatgc gtgttgtagg
     3841 gatactgtta gtaagtgctt taatcacgtt accaattgca ataagtatgc gaataacaaa
     3901 aagttttaag caattgatat tactaagtgt atttttaggt gaattatcag taatattagg
     3961 cttagtccta gctttctata tggacatttc tccgggtggt gtaattgtag ttctactagt
     4021 tattttactt atgattacaa tggcttatca gaaaatgcga atgaagttta aaaagggagc
     4081 taatatcaat gaatacaaat gatgctatta aaattttaaa agagaacggt ttaaaatata
     4141 cagataaacg taaagatatg ttagatattt ttgtcgaaga agataagtat ataaacgcaa
     4201 agtatataca acaagttatg gatgaaaatt atcctggaat ttcattcgac acaatatata
     4261 gaaacctgca cttatttaaa gatttaggga ttattgaaaa tacagaactt gatggtgaaa
     4321 tgaagtttag aatcgcttgt acaaaccatc atcatcatca ttttatctgt gaaaagtgtg
     4381 gagatacaaa ggtaatagat tattgtccaa tagatcagat aaagttatca ctacctggtg
     4441 ttaatattca caaacacaaa cttgaagttt atggtgtatg tgagtcttgc caagattaat
     4501 ataaagaaat gagatttatg cacatttggt cagatgtatg cataaatctc attttttaaa
     4561 ttatagaaga taaaggttca tttcatactc cg
//
LOCUS       DANBWH010000195         4582 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.203, whole genome shotgun sequence.
ACCESSION   DANBWH010000195 DANBWH010000000
VERSION     DANBWH010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.203"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            98..559
                     /gene="tsaE"
                     /locus_tag="PPY92_002300"
     CDS             98..559
                     /gene="tsaE"
                     /locus_tag="PPY92_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDJ2626277.1"
                     /translation="MIKINNLDEMNQFAMFLVEQLKSGDLILLNGDLGAGKTTLTQFI
                     GKALGVRRTINSPTFNIIKSYRGKNLKLHHMDCYRLEDSDEDLGFDEFFEDQAITVIE
                     WSQFIKDLLPATHLSINISTISENTRQIELFAQGEHYEQIKEAIIHEFAAH"
     gene            540..1202
                     /gene="tsaB"
                     /locus_tag="PPY92_002301"
     CDS             540..1202
                     /gene="tsaB"
                     /locus_tag="PPY92_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDJ2626278.1"
                     /translation="MNSLLIDTSNQPLSVALMQNDEVLAEITTDSKQNHSVQLMPAIS
                     QLFEQSKIAKQQLDAIIVAEGPGSYTGLRIGVTVAKTLAYALDVKLYGVSSLKALAAT
                     IDHTDKLLVPVFDARRQAVYTGIFQWQNGQLVTILEDQYMSIEALRAFLEDLNQPFIY
                     IGKDTIKLQDELQGEVIAQLPNASVMYQLIDKPTDVHTFTPKYHKLAEAERNWINSQK
                     NN"
     gene            1175..1639
                     /gene="rimI"
                     /locus_tag="PPY92_002302"
     CDS             1175..1639
                     /gene="rimI"
                     /locus_tag="PPY92_002302"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDJ2626279.1"
                     /translation="MDQQSKEQLNIREMTKEDVPQVFDLERRSFNDSSWTIDAFYHEI
                     EQNNFAKYFVLEFEQQIIGYLGLWIVIDQAQITTVAIDDNYRGYGLGQMLLKYGINYA
                     SHTCDVMSLEVRVNNKVAQHVYENLGFQYGGKRKNYYGEGEDAMVMWVNLND"
     gene            1632..2657
                     /gene="tsaD"
                     /locus_tag="PPY92_002303"
     CDS             1632..2657
                     /gene="tsaD"
                     /locus_tag="PPY92_002303"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDJ2626280.1"
                     /translation="MTKDILILAVETSCDETSVSVIKNGRDILSNTVLSQIESHKRFG
                     GVVPEVASRHHVEGITTTINEALVDADVSMEDIDAIAVTEGPGLIGALLIGVNAAKAL
                     AFAYDKPLIPVHHIAGHIYANHIEEPLTFPLIALIVSGGHTELVYMKDHLSFEVIGET
                     RDDAVGEAYDKVARTIGLNYPGGPQVDRLAAEGEDTYSFPRVWLDKDSYDFSFSGLKS
                     AVINQLHNQRQKNIPIIEANVATSFQNSVVEVLTFKAIQACKEYSVQRLIVAGGVASN
                     KGLRQSLADQCKVNDIQLTIPSPKLCTDNAAMIGVAGHSLYQQGRFADLALNGHSNID
                     LEEYSAE"
     gene            complement(2972..4582)
                     /locus_tag="PPY92_002304"
     CDS             complement(2972..4582)
                     /locus_tag="PPY92_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HDJ2626281.1"
                     /translation="MSTNQTFLIFVIAIILLTSVIGIVGRYMSRQRLLKSMETLWQTI
                     SPLEAFIRPNSHFDYEYKLYKEKFESHSLVDDKTWSDLNMNAIFHKMNYNLTAIGEMK
                     LYACLRGMLSITNKSLLSLFNDNAEFRKNVTYHLALIGKTVYPTFPDQITPVKRQNIL
                     FLCPFLPVISFAVIFINSQVGILLFLMSCLFNIILSATLKRTYEDDLKSIFYASNVLK
                     QGYTISKIKHAPQPEVNFKQFRTARHLTSVLAEVNDEDIGAMVIKLVKLIFMLDYVLF
                     HSIQKSYTTHMNELKNCFDYIAELDNHYALAMYRRTLECYTEPQIDDTNDGIVFSELT
                     HPLIADAVANDFSLSQNILLTGSNASGKSTFMKSIAINIILASAIQTVTASKFVYQPG
                     IVFTSMANADDVLSGDSYFMAELKSIKRIVEIPDNQKIYCFIDEIFKGTNTTERIAAS
                     ESVLSFLHEKSNFRVIAATHDIELAELLKQRYENYHFNEVIENNNIHFDYKIKPGKAN
                     TRNAIELLKITSFPAKIYERAKDNVSNG"
BASE COUNT         1565 a          658 c          858 g         1501 t
ORIGIN      
        1 atttgttaca atataaaggt taaatataaa gttagatacg attgaacaat ggataatcat
       61 ttttatgaat gaaaagcata atataggaga atcaacattg ataaagataa ataatttaga
      121 tgaaatgaat caatttgcta tgtttttagt tgagcaattg aaaagtggtg atttgatttt
      181 acttaacgga gatttaggag caggtaaaac aacgttaacg caatttatag gaaaagctct
      241 tggtgtaaga cgtacgatta attccccgac atttaacatc attaaatcat ataggggtaa
      301 aaatttaaaa ttgcatcata tggattgtta tcgcttagaa gattctgatg aagatttagg
      361 gtttgatgaa tttttcgaag atcaggcaat tactgttatt gaatggagtc aatttataaa
      421 agatttactt ccagcgacgc atttatctat taacatttca acaatatctg aaaatacaag
      481 acaaattgag ttgttcgcgc aaggagaaca ttatgaacaa attaaggagg caattatcca
      541 tgaattcgct gctcattgat acatcgaatc aaccattatc agtagcattg atgcaaaacg
      601 atgaagtttt agctgaaata actacggatt ctaaacaaaa tcattcagtg caattaatgc
      661 ctgcgattag tcagctattt gagcagagca aaatagctaa acagcaatta gatgcgatta
      721 ttgttgctga aggtcctggt tcatatactg gcttacgtat cggtgttact gttgctaaaa
      781 cattagcgta tgcgttagat gttaaattat atggtgtttc atcattaaag gcattggcag
      841 caacaatcga tcatactgat aaattattgg ttcctgtgtt tgatgcaaga cgacaagcag
      901 tttatacagg catattccag tggcaaaatg gtcaattagt gactatttta gaagatcaat
      961 atatgtcgat agaggcattg cgagcttttt tagaagattt aaaccaacca tttatttata
     1021 ttgggaaaga tactataaaa ttacaagatg aattacaagg tgaagtgatt gcgcagctac
     1081 caaatgcatc agtcatgtat caattgatag ataaacctac agacgtacat acatttactc
     1141 ctaaatacca taaattagct gaggcggaac gaaattggat caacagtcaa aagaacaatt
     1201 aaacattaga gagatgacta aagaagatgt gccacaagtc tttgatcttg agcgtcggag
     1261 tttcaatgac agttcttgga caattgacgc attttatcat gaaatagagc aaaataactt
     1321 tgcgaaatat tttgtcttag aatttgaaca acaaattatt ggttatttag gtttatggat
     1381 tgttatcgat caagctcaaa ttacaacagt tgcaatcgat gataattata gaggctatgg
     1441 tttaggtcaa atgcttttaa aatacggaat aaactatgca agtcacactt gtgatgtgat
     1501 gagtttagaa gtaagagtta ataataaagt ggcacaacat gtatatgaaa atttaggatt
     1561 tcaatatggt ggtaaacgta aaaattatta tggtgaaggt gaggacgcaa tggttatgtg
     1621 ggtgaattta aatgactaaa gatatattaa tactagctgt tgaaacaagt tgtgatgaaa
     1681 caagcgttag tgttataaaa aatggcagag atattttatc aaatacagtt ttaagtcaga
     1741 ttgaaagtca taaacgattt ggcggtgtcg ttcccgaagt ggcaagtaga catcacgttg
     1801 aaggtataac aacaacaata aacgaggctc tagtggatgc cgatgtatca atggaagata
     1861 ttgatgccat agcggttaca gaaggccctg gactaattgg tgcgttacta ataggtgtta
     1921 atgcagccaa agcattggca tttgcttacg ataagccact tattcctgtt catcatattg
     1981 caggacatat atatgctaat cacatagaag agccattaac attcccgcta attgcactta
     2041 ttgtttcagg tggacatact gaattagttt atatgaaaga tcatttatca tttgaagtca
     2101 ttggtgaaac acgagatgac gcagtaggtg aggcttatga taaagtggca cgaacaattg
     2161 gtttaaatta tccaggtggt ccacaagttg atcggttggc tgctgaaggt gaagatactt
     2221 attcattccc tcgtgtttgg ttggataaag atagttatga ttttagtttt agtgggttga
     2281 aaagtgccgt gatcaatcaa cttcacaatc aacgacaaaa aaatattcca atcattgaag
     2341 ctaacgtagc aacgagcttt caaaatagtg ttgtagaggt gcttacgttt aaagctattc
     2401 aagcttgtaa agaatatagt gttcagcgat taattgttgc tggtggcgtg gcgagtaata
     2461 aaggattacg tcaatcttta gcggatcaat gcaaagtcaa tgacattcaa ttaactatcc
     2521 caagtcctaa attatgcaca gataatgctg caatgatagg cgttgccggc cactctttgt
     2581 atcagcaagg tcgatttgct gatttagcat taaatgggca cagcaatata gatttagaag
     2641 agtattctgc agaataaaat aaacgtcagt tattaacatg attacgatga acattattga
     2701 aactgttcat agtcttctgt catgtgatag ctgacgtttt tatattgcaa ttaggggagt
     2761 gagacagaaa agataaagaa ccactaatga tttattatgt tgggattctg ccaacttgca
     2821 cattattgta agctgacttt ccgccagcct ctgtattggg gcccccggac tagtattgaa
     2881 aaaacttatt acaagcgtat tttcatttag tcaaagactg tcaatataac aatgttgcga
     2941 cttgaacatt gattgatgtt caagattcat gctaaccatt ggaaacatta tcttttgctc
     3001 gttcatatat ttttgctgga aatgaagtga tttttaataa ttcgatggca ttacgagtat
     3061 ttgctttgcc aggcttaatt ttgtaatcaa aatgtatgtt attattttct attacctcat
     3121 tgaaatgata attttcataa cgttgtttta agagttcagc taactcaata tcatgtgttg
     3181 ctgcaataac tctaaagtta gatttttcat gtaaaaatga tagtactgat tctgaagcgg
     3241 caattcgttc agttgtgttg gtacctttaa aaatttcatc gataaagcag tatatttttt
     3301 gattatctgg aatttcaaca atacgcttaa tagattttaa ttcagccata aaataactat
     3361 cgcctgataa cacatcatcg gcatttgcca ttgacgtgaa cacaattcca ggttgatata
     3421 caaatttgct tgcggtaact gtttgtatag ccgatgcaag aataatgttt attgctatgg
     3481 atttcataaa tgttgattta cccgaagcat tagacccagt taacagtata ttttgtgaaa
     3541 gtgagaaatc attggctact gcatctgcta tgagtggatg tgttaactca gagaatacta
     3601 tgccatcatt tgtatcatca atttgtggtt cagtataaca ttctagcgtt ctacgataca
     3661 ttgctaatgc ataatgatta tctaattcag ctatgtaatc gaaacaattt ttaagctcat
     3721 tcatatgtgt tgtatagctt ttttgaattg aatgaaataa aacataatca agcatgaaaa
     3781 taagtttaac tagtttaatt accatagctc cgatatcttc atcattaact tcagctaaaa
     3841 cactcgttaa atgacgtgct gttctaaatt gcttaaaatt gacttcaggt tgtggagcat
     3901 gtttaatttt tgagatagta tagccttgtt ttaaaacatt ggatgcataa aagatagatt
     3961 ttaagtcatc ctcatatgta cgctttaaag ttgctgataa aatgatatta aataggcaac
     4021 tcataagaaa taataaaata ccaacttgtg aattaataaa tataactgca aatgagataa
     4081 ctggtaaaaa cgggcataga aacaatatat tttgacgttt taccggtgtg atttggtctg
     4141 gaaatgttgg ataaacagtt ttaccaatca aagctaaatg atatgttacg ttttttctaa
     4201 attcagcatt atcattaaat aaactaagta atgatttgtt cgtaattgaa agcattccac
     4261 gtaaacaggc atatagcttc atttcaccaa tagctgttaa attataattc atcttatgaa
     4321 aaattgcatt catatttaag tcggaccaag ttttatcatc aactaatgaa tgtgattcaa
     4381 atttttcctt gtagagctta tactcatagt cgaaatgtga gttcggtctg ataaaagctt
     4441 ctaatggaga aatcgtttgc cataatgttt ccatagattt taatagacgt tgacgactca
     4501 tgtatcgtcc aacaattcct attacagatg taagtagaat aattgctata acaaatatta
     4561 aaaatgtttg atttgtgctc at
//
LOCUS       DANBWH010000196         4534 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.204, whole genome shotgun sequence.
ACCESSION   DANBWH010000196 DANBWH010000000
VERSION     DANBWH010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.204"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..3398
                     /locus_tag="PPY92_002305"
     CDS             <1..3398
                     /locus_tag="PPY92_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HDJ2626282.1"
                     /translation="EVEKSRNEALLHTINQVIKKVYLQQYTCSFVGHFSAGKSTLINL
                     LIEQDILPSSPVPTTSNTAIVSVSDNHDIIANLPNQTYAKLSNYDEVREMNRQNVDVE
                     SVEINFQSAKFENGFTLQDTPGVDSNVASHQSITEQYMYTSNMIFYTVDYNHVQSELN
                     FKFMKHINDVGIPVVFIINQIDKHQDDELSFSTFKSRVEKSIADWGIKLERTFYVSKF
                     DHPENELEVLSSYLISLDQHRETIEDYTSRTVEYITEAQLDYIQSEIQEVLEDLGIEE
                     AEFEQAFLNSQQHQAISEEAQLLNNPDELMAFLKNKRKNILENAYIMPHNMREMLRSY
                     LESMSQDFNVGGFFNKKKKKLQIQQQRLLTATDALQEHVNQQIRQPMREDMSFVTRFI
                     NKKEASDKVLNQHYDVKPEMIEDLYQPQTSISNTYVLTFSDEVVKAIKKYVEQQSTPI
                     FKEIIENVQADELPTEESDDLKEYQRYTELNELRQSLTTKNYRHYYIHLDESLDKLIG
                     RQETTYQVATDNTQDNRDNQQLNQNTATTNMSIDIQKALDIISDVPLFKRTKQDIHET
                     LTRIDNKLIKIGVFGTFSAGKSSLINALLGEHILVSSPNPTTAATTEISYGDESYITL
                     KSQSQLLDEINAVVEYQDISFSTIEEFINWDLEKLKSHLNKNQLAFIQAVEKHYKLYV
                     NMLENGEKHAINQQELKKWSAEDEYATFVKTVHIALMHDWLKGKIIVDSLGLHSNNQR
                     HTNETEQILTSSDLILYVSYFNHSFTDNDKAFIEHMKDMNQLNENQAFKMVINAADLA
                     ESQDDLEAVETYVSDALRQVHLQSDIFAVSSRNALQAEDKGIDQLKQSIQQFVDVESK
                     SILEQQMIHQLQQMDRSYVEMITEFETNKADISRRQQRLTDYKDKQRLQHQLIDATLQ
                     HTDNEVEEQVYHLNARLKLQLLDDVKSVFNSQMTQNSDFNEEKKVSTKVYLDQIHQRL
                     FLEQSLITERIKKYFNKQLTKQIAPIVQQLADLHVIVNPQFNFESANIEQPLLHIDFN
                     DMLNALPKQLTKRKILNPNGQRDIHESICQSTLGLLQPQMGLLRQQLELYVKQMAVEA
                     ESQFESFEANIQTQINDLLAFDLDTTLINQLKDKHQQLKTILY"
     gene            3418..4296
                     /locus_tag="PPY92_002306"
     CDS             3418..4296
                     /locus_tag="PPY92_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HDJ2626283.1"
                     /translation="MPNKILLVDGMALLFRHFYATSLHKQFMYNSQGVPTNGIQGFVR
                     HIFSAIHEIRPTHVAVCWDMGQSTFRNDMFDGYKQNRSAPPEELIPQFDYVKEISEQF
                     GFVNIGVKNYEADDVIGTLAQQYSTDNDVYIITGDKDLLQCINDNVEVWLIKKGFNIY
                     NRYTLHRFNEEYALEPQQLIDIKAFMGDTADGYAGVKGIGEKTAIKLIQQYQSVENVV
                     ENIDALSAGQRNKINDNLDELYLSKRLAEIHTQVPIDSEALFEKMSFATTLNHILSIC
                     NEHELHVSGKYISSHF"
BASE COUNT         1786 a          645 c          737 g         1366 t
ORIGIN      
        1 aagaagttga aaagtcgcga aatgaagcac ttttacatac aattaaccaa gtaattaaga
       61 aagtatattt gcagcaatat acatgttcgt tcgttggaca tttttctgca ggtaaatcga
      121 cactgataaa tttattaatt gaacaagata tcttaccaag ttcacctgta ccaacgacaa
      181 gtaatactgc tattgtgtca gtttcagaca atcacgatat tattgctaat ttgccgaatc
      241 aaacatatgc caaattatct aattatgatg aagtaaggga aatgaatcgc caaaatgtcg
      301 acgttgaatc tgtagaaatt aattttcaat cagctaaatt tgaaaatggg tttacgttgc
      361 aagatacacc aggtgttgat tcaaatgttg catcacatca gtcaataaca gaacaatata
      421 tgtatacaag taatatgata ttttatacgg ttgactataa ccacgttcaa tctgaactta
      481 actttaagtt tatgaagcat ataaatgatg ttggaatacc tgttgtgttt atcattaatc
      541 aaattgacaa gcatcaagac gatgaattgt cattctctac gtttaaatca cgagttgaaa
      601 aatcaattgc agattggggc attaaattag aacgcacctt ttatgtatct aaatttgatc
      661 accctgaaaa tgaacttgaa gtattatcaa gttatctaat ttcattagat caacatagag
      721 agacaataga ggattataca tcaagaacgg ttgaatacat taccgaagct cagctagatt
      781 acattcagtc tgaaattcag gaagtactag aagatttagg tatcgaagaa gcggagtttg
      841 aacaagcatt tttaaatagt caacaacatc aagcaattag tgaagaggca caacttttaa
      901 ataatccaga tgaattaatg gcatttttaa agaataagcg taaaaatatt ttagaaaatg
      961 catacattat gccgcataat atgagagaaa tgttacgaag ttatttggaa agtatgtctc
     1021 aagattttaa tgttggtggg ttttttaata aaaagaagaa aaagctacaa attcaacaac
     1081 agcgattatt aacagcgaca gatgcgttac aagaacatgt taatcaacaa attcgtcaac
     1141 caatgcgaga agatatgtca tttgtaacgc gttttatcaa taaaaaagaa gcttcagata
     1201 aagtattaaa tcagcattat gacgttaaac cggaaatgat tgaagattta tatcaaccac
     1261 aaacatcaat cagcaatact tatgtactta cattttcaga cgaagtggtt aaagccatta
     1321 agaaatatgt tgaacaacaa tcaacaccga tttttaaaga aataatagaa aatgtgcagg
     1381 cagatgaatt accaacagaa gaaagtgatg atttaaaaga atatcaacgt tatacagaat
     1441 taaatgagct gcgtcagtca ttgacgacta agaattatcg tcactactat attcatttag
     1501 atgaatctct agataaatta ataggtcgac aagagacaac atatcaagtg gctactgata
     1561 atactcagga taatcgtgat aatcagcagc taaatcaaaa tacagctaca acaaatatgt
     1621 ctatagatat tcaaaaagcg cttgatataa tttcggatgt gcctttgttc aagcgtacaa
     1681 agcaagatat ccacgaaaca ttaacacgta tagataataa attaataaaa ataggtgtat
     1741 ttggaacatt tagtgcgggt aaaagtagtt tgataaatgc attattaggc gagcatattt
     1801 tagtgagttc tccaaatcct actacagcag ctactacaga aatatcttat ggagacgaga
     1861 gttatataac gctaaaatca caatcgcaat tattagatga aattaatgca gtagttgaat
     1921 accaagatat atctttttca actatagaag aatttattaa ttgggattta gaaaagttaa
     1981 aatcacattt aaataaaaat caactcgctt ttattcaggc agttgaaaaa cattataaat
     2041 tgtatgtcaa tatgttggaa aatggagaaa aacatgccat taatcaacag gaattgaaaa
     2101 agtggagtgc agaagatgaa tatgcaacat ttgttaagac agtacacatt gcattaatgc
     2161 atgattggtt aaaaggtaaa ataattgttg attcattagg gctacactca aataaccaaa
     2221 ggcatacaaa tgaaaccgaa caaattttaa cttcttcaga cttaatattg tatgtaagtt
     2281 attttaatca ttcatttact gataatgaca aagcgtttat agaacacatg aaagatatga
     2341 accagttgaa tgaaaaccaa gcatttaaaa tggtaattaa tgctgctgat ttagcagaaa
     2401 gtcaagatga tcttgaagca gttgaaacat atgtatcaga tgcattaaga caagtacact
     2461 tacaatcaga catttttgct gtatcaagtc gaaatgcatt gcaagctgaa gataagggca
     2521 ttgatcaatt aaaacaaagc atacaacaat ttgttgatgt tgaatctaaa tcaattttag
     2581 aacaacaaat gattcatcag cttcaacaaa tggatcgttc ttatgtagag atgattacag
     2641 aatttgaaac aaataaagct gatatttcac gtcgccaaca aaggttaaca gattataaag
     2701 ataaacagcg tttacaacat caattaattg atgcgacgct acaacataca gacaacgaag
     2761 ttgaagaaca agtttatcat ttaaatgcac gtttaaaatt acaactactt gacgatgtta
     2821 aatcagtgtt taattctcaa atgacgcaaa atagtgattt taatgaagaa aagaaagtgt
     2881 ctacgaaagt atacttagat caaattcatc aacgattgtt tttagagcaa tctttaatta
     2941 cagaacgtat aaaaaaatac tttaataagc aactcactaa gcaaattgca ccaatcgttc
     3001 aacaattagc agatttacat gtcattgtta atcctcagtt taactttgaa tcagctaata
     3061 tagagcaacc attattgcac atcgatttca atgatatgct aaatgcattg cctaaacaat
     3121 taacaaaacg taaaattttg aatccaaatg ggcaaagaga tatacatgaa tcaatttgtc
     3181 aaagtacgtt aggattatta caaccacaaa tgggattatt gaggcaacag cttgaattat
     3241 atgtaaagca aatggctgta gaagctgaat cgcaatttga aagttttgaa gctaatattc
     3301 aaacgcaaat aaacgattta ttagcatttg atttagatac aacacttatc aatcaattga
     3361 aagataaaca tcaacaactg aaaactattt tatattaaga aagaaggaac gttttaaatg
     3421 cctaataaaa tattacttgt agatggtatg gcgctattat ttagacattt ctatgctaca
     3481 agtcttcata aacaatttat gtacaattca caaggtgtac ctacaaatgg aatacaagga
     3541 tttgtgcgtc atatcttttc ggcaatacat gaaatacgcc ctacacatgt agctgtatgt
     3601 tgggatatgg gacaatcaac ttttagaaat gatatgtttg atggttataa gcaaaatcgt
     3661 tctgcaccac cagaagaatt gataccacaa tttgattatg ttaaagaaat ttcagagcaa
     3721 tttggctttg taaatattgg cgttaaaaac tatgaagcgg atgatgttat aggtacatta
     3781 gcacaacaat attcaactga taacgatgtc tatattatta cgggcgacaa agatttactg
     3841 caatgtatta atgacaatgt tgaagtctgg ctaattaaaa aaggttttaa catttataat
     3901 agatatacat tacatcgttt taacgaagaa tatgcccttg aaccacaaca actaatcgat
     3961 attaaagcgt ttatgggtga tacagcagat ggatatgcag gtgttaaagg aattggtgag
     4021 aaaacggcta ttaagttaat tcagcaatat caaagcgttg aaaatgtggt tgaaaacatt
     4081 gatgcattat cagctgggca acgtaataag attaatgaca atttagatga actatattta
     4141 tcgaagcgac ttgctgaaat tcacacacaa gtaccaattg atagtgaagc gctttttgag
     4201 aaaatgtcat ttgcaacaac acttaatcat attttatcaa tttgtaacga acatgaatta
     4261 catgtttcag gaaaatatat ttcatcccat ttttaaaaaa gtataaaatt ttatgaaaaa
     4321 ttaaaccaat taagtataaa aagactattt ctatcgatgc aaggtagaaa tagtcttttt
     4381 gcgtatttgg atatgtcaga gtgaataaat aagacgaatt ttgatagata catatttgat
     4441 taatcaatag agtttaaaga attatatttc atccaaataa attaccatac actacaaaat
     4501 agtacagtta caactttatt aatctaaaaa acat
//
LOCUS       DANBWH010000197         4522 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.205, whole genome shotgun sequence.
ACCESSION   DANBWH010000197 DANBWH010000000
VERSION     DANBWH010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.205"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(218..313)
                     /locus_tag="PPY92_002307"
     CDS             complement(218..313)
                     /locus_tag="PPY92_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626284.1"
                     /translation="MGGFVNSLIMKDLSDNDMRFKYEYYNREKDT"
     gene            complement(389..994)
                     /locus_tag="PPY92_002308"
     CDS             complement(389..994)
                     /locus_tag="PPY92_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HDJ2626285.1"
                     /translation="MNMPLMIDLTNKNVVIVGGGVVASRRAQTLNQYVEHMTVISPTI
                     TEKLQNMVDNGVVIWKEKEFEPSDIVDAYLVIAATNEPRVNEAVKQALPEHALFNNVG
                     DASNGNVVFPSALHRDKLTISVSTDGASPKLTKSIMAELEALYPPSYSSYIDFLYTCR
                     QKIKVLDITYNEKQQLLSQIVSQEYLNHDKQAQFLAWLDVR"
     gene            complement(1057..2937)
                     /locus_tag="PPY92_002309"
     CDS             complement(1057..2937)
                     /locus_tag="PPY92_002309"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HDJ2626286.1"
                     /translation="MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSNDT
                     TSDSNQQQLETEVWQQSQISEEQATSTTYMLQNKEPHIEANQRHVTVLYGSESGNAMR
                     LAEIFSERLSDIGHQVVLMSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFL
                     EDDNAPNLNHVRYSVLALGDQTYEFFCQAGKDVDALLENLGAERICKRVDCDIDYEED
                     AEKWMADIINMIDTTSEGIQSESVISESIKSAKEKKYSKLNPYQAEVLANINLNGTDS
                     NKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLISMLGWDPQSPVPINDRGDT
                     VPIVEALTSHFEFTKLTLPLLKNADIYFDNEELSERIQDESWAREYVINRDFIDLITD
                     FPTIELQPENMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSV
                     HFAERIKPGDIVPIYLKKNPNFKFPMKQDIPVIMIGPGTGIAPFRAYLQEREELGMTG
                     KTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIMEESERF
                     NEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQEEAELLLRQMKQQQRYQRD
                     VY"
     gene            3131..3223
                     /locus_tag="PPY92_002310"
     CDS             3131..3223
                     /locus_tag="PPY92_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626287.1"
                     /translation="MSVINAYSNASLLFFIYLLFIMYQISLSVK"
     gene            complement(3331..3828)
                     /locus_tag="PPY92_002311"
     CDS             complement(3331..3828)
                     /locus_tag="PPY92_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDJ2626288.1"
                     /translation="MNNRNVYDIEVSDYKGLTYKLEAFRGKVILVVNTATECIYSEQL
                     KKLETLFQKYKDRGFVVLSFPNNNFDNRQPGSNEEILKISREKFGVTFPVLAKISVNG
                     NDEHPLFTHLKNEQPGIFGSPIKWNFTKFIIDRQGNVVNRFLPMEDPLDISTNIEILL
                     QESSS"
     gene            complement(3838..4098)
                     /locus_tag="PPY92_002312"
     CDS             complement(3838..4098)
                     /locus_tag="PPY92_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626289.1"
                     /translation="MIIMRGYNERYCSWTNTAIQCDYFNKVRQVKKDGKSDRLSNNIN
                     SNIIYRVLEKCITLYNVCSRCKRALILSEHSKKNIFNIKKAV"
BASE COUNT         1363 a          854 c          626 g         1679 t
ORIGIN      
        1 aaaaaaatct tttttgcgta actatcaata gtgaagtttg attttcctac ttgataacat
       61 ggcatgacta atccttgtat atcctgtcct gtatttcatt ttgatctatc atttttatta
      121 ttgctttcta ttgcacaaaa aatctggcta aaggtcgcta tgatagtaac gacttcaacc
      181 agatttcaat ttatccatct attttattaa gtttgttcta cgtatctttt tctctattgt
      241 aatattcata tttaaaacgc atatcattat cgcttaaatc cttcattatt agactattca
      301 caaagccacc catgtcttaa tttcaatgat taagcgttaa agttaaaata agaagactta
      361 ccttagacgg ttagacggtc cgctattatt atcttacatc caaccacgct aaaaattgag
      421 cttgtttgtc atgatttaaa tattcttgtg acacaatttg tgacagtaac tgttgctttt
      481 cgttatatgt tatatcaagt acttttattt tctgtcggca agtatataaa aagtcgatat
      541 acgaactgta tgatggtgga tataacgcct caagctctgc cataattgat tttgtcaact
      601 taggactcgc accatcagtt gatacactga tagttagctt gtcgcggtgt agtgcacttg
      661 gaaatacaac attgccattt gatgcatctc caacattatt aaaaagggca tgctcaggta
      721 aggcttgttt taccgcttca ttgacacgtg gctcattggt tgctgcaata actagatacg
      781 cgtctacaat atcgcttggt tcaaattctt tttctttcca tatgacgaca ccgttatcta
      841 ccatattttg aagtttttca gtgattgtcg gactgatgac cgtcatatgt tcaacgtatt
      901 gatttaatgt ttgtgcccga cgacttgcaa cgacgcctcc accaactatg acgacatttt
      961 tatttgttaa atctatcatt aatggcatat tcatgttgag caacatcctt tatatctttt
     1021 tacatcatta caccttaaaa tatttaacac caatcgctaa taaacatcac gttgatagcg
     1081 ttgttgttgt ttcatttgtc gcaataataa ctctgcttct tcttgagaaa tatgacgttc
     1141 ttttaccaat acatctttaa tggcttgatg gacatctttc gccatacatt tttcatcgcc
     1201 acaaatatag attgctgcgc cttgctcaat ccattcattg aaacgttcac tttcttccat
     1261 tatacgatgc tgtacatatt ctttgtgttc ttggtctctt gaaaatgcta aatctacgcg
     1321 tgttaagttt ccattttcaa gccattcttc tatttcttct tcatataaaa agtcagaact
     1381 acggtgttga tcaccaaaga acaaccatgt ttttccagtc ataccaagtt cttcacgttc
     1441 ttgtaaatat gctctaaaag gagcaattcc agtacctggt ccaatcataa taaccggtat
     1501 atcttgcttc atcggaaatt tgaagttcgg atttttcttc aaataaattg gtactatatc
     1561 gcctggttta attcgctcag caaaatgaac cgagcataca ccttttctct cacgtccatg
     1621 tgcttgataa cgaaccgtac caacggtaat atgcacttca tctggcgttg ccataaaact
     1681 actagaaatc gaatactctc ttggtggtaa ttttctaagg atttgataca tattctcagg
     1741 ttgtaattct atagttggaa aatctgttat taaatctata aagtcccgat ttataacata
     1801 ttcacgcgcc catgactcat cttgaatacg ttcagataat tcttcattgt caaaatagat
     1861 atctgcattt ttcaataatg gcaatgttaa tttagtaaat tcaaaatgtg atgttagtgc
     1921 ttcaacaata ggaactgtat caccacgatc attaattggc accggagatt gcggatccca
     1981 acctaacatg gatattagtt tttcaaccaa ttcagggtcg ttttgcggta atgctactat
     2041 acaatctcct ggttcatatg attcactaaa atcatcaagt aaaaattcta tatgtcgtgt
     2101 ttctttattt gaatcggtac catttaaatt gatattcgct aaaacttctg cttggtatgg
     2161 atttaattta gaatatttct tttctttggc agacttaatt gattcactta tcaccgattc
     2221 actttgaata ccttctgatg tagtatcaat catattaatg atgtctgcca tccacttttc
     2281 tgcgtcttct tcataatcaa tatcacaatc tacacgctta catatacgct cagcgcctag
     2341 attttctagt aaagcatcta catctttacc agcttgacag aaaaattcat atgtttgatc
     2401 acctaaagct agtactgaat atctcacatg atttaaatta ggtgcgttat cgtcttctaa
     2461 aaattcaaag aaatcccatg cattatcagg cggttctcct tcaccatgag tagacgtaat
     2521 aataaataaa tcttctaact gtgcgatgtt tgtcgtatca tattcatcca ttgacatcaa
     2581 aacaacttga tgtccgatat cacttaaacg ttctgaaaaa atttcagcta aacgcatggc
     2641 attacctgat tcagaaccat atagcactgt aacatgccgc tgattagctt cgatatgtgg
     2701 ctctttattt tgtaacatat acgttgttga agttgcttgt tcttctgata tttgtgattg
     2761 ttgccacact tctgtctcta attgttgttg attactatca cttgtcgtat cgttactatt
     2821 agctagtaaa aaaccactaa gccactgttg ttggctctct gttaatgttt gcagcagatt
     2881 gttaatctct gtgacttgtt tttctgtaaa tggactatta gatgtattta atttcaaatg
     2941 tttcacctca ataaacttga aatagatgat tgactctttt tttatcgata gtttagtcaa
     3001 ttttttaata tgtatggtat aaattaaaac aaacttgagt tgtgtctgat agttatactt
     3061 gcaaatattt acaactttca tgttgcttga atgacattat cagtaattca gacaaaactt
     3121 tgattatgtc gtgtcagtta ttaatgcata cagtaatgca tcgttgttat tttttattta
     3181 tttactattt attatgtacc aaatcagcct ttcagtaaaa taggtcaaat tactgatttt
     3241 ctaaatataa aatgcctcct aatgacatac tattagtaca tcattaagag gctcttgtgt
     3301 tatttgcata ctaagcgctc aattttaaat ttaagatgaa gattcttgca ataatatttc
     3361 tatatttgtc gatatatcca atggatcttc cattggcaag aatcgattta caacattgcc
     3421 ttgtcgatcg attataaatt ttgtgaaatt ccattttatt ggggacccaa agattcctgg
     3481 ttgttcattc tttaaatgcg taaataacgg atgttcatcg ttcccgttca cagatatttt
     3541 agctagcact ggaaatgtaa caccaaattt ctcacgacta attttcaaga tttcttcatt
     3601 agaacctggt tgtcgattgt caaaattatt attcggaaaa ctcaacacta caaacccacg
     3661 atccttatat ttttgaaaca aagtctctag ttttttcaat tgttcgctat atatacattc
     3721 tgttgcagta ttaacaacta aaatcacttt acctctaaat gcttctaatt tataagttaa
     3781 gcctttataa tcacttactt cgatatcata cacatttcta ttattcataa gacaccccta
     3841 cacagccttt tttatattga atatattctt tttagaatgt tctgataaaa taagtgcgcg
     3901 tttacaccgc gaacatacat tatatagcgt gatacatttt tcgagcacac gataaataat
     3961 gttcgagttt atgttgttgc tcaacctatc cgatttaccg tcttttttca cctgtcttac
     4021 tttattaaaa taatcacatt gtatagctgt attagtccag ctacaatatc tttcgttata
     4081 acccctcatt ataatcatcc ttattttcta tttttaaaaa gacaattaga ccgctcttta
     4141 aactatagat taatacttaa gttaaactca tacatactga taccatacgt tagatctaac
     4201 aatttaaaat tcgttataac tatggattaa agagctgccc aactcatata atccttaaaa
     4261 acttcacatg tgattgttta ttaagccctc ctttatctta ttaaatatcc ttataaccct
     4321 tttaaaatta aactgacaca ctcatacatt gttacacgcg acgataaacg tcatattatt
     4381 gcatatcatt aaaacaagtc actttgtatc gtgtgcgacc gaatacatat taaatcatca
     4441 ataatgatta ttcaattatt aaaaataaaa acgaacattc tatatagagt ttctacttat
     4501 tgttttttca gcattgtcta ca
//
LOCUS       DANBWH010000198         4512 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.206, whole genome shotgun sequence.
ACCESSION   DANBWH010000198 DANBWH010000000
VERSION     DANBWH010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.206"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            86..598
                     /locus_tag="PPY92_002313"
     CDS             86..598
                     /locus_tag="PPY92_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HDJ2626290.1"
                     /translation="MTLFLLEANNLDFASTKEELEAKAASLSTKTIPTLIEVQATENL
                     THGYFIVEANDEAEAKQFLTEADISIQLVKEVRLVGKDLDEVKNGDAHVDYLVTWNIP
                     EGITMDQYLARKKKNSVHYEEVPEVEFKRTYVCEDMSKCICLYNAPDEEAVRRARKAV
                     DTPIDGIEKL"
     gene            940..1680
                     /locus_tag="PPY92_002314"
     CDS             940..1680
                     /locus_tag="PPY92_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDJ2626291.1"
                     /translation="MIKIQQLQHHFGSHKVIHNFNLDISKGEIVTFIGKSGCGKSTLL
                     NVIGGFIHPSSGRVIIDNEIKQQPSPDCLMLFQHHNLLPWKTINDNIRIGFQQKISDE
                     EINAQLKLVDLEDRGKHFPEQLSGGMKQRVALCRAHVHKPNVILMDEPLGALDAFTRY
                     KLQDQLVQLKHKTQSTIILVTHDIDEAIYLSDRIVLLGEGCNIISQYEITASHPRSRN
                     DSHLLKIRNEIMETFALNHHQVEPEYYL"
     gene            1694..2665
                     /locus_tag="PPY92_002315"
     CDS             1694..2665
                     /locus_tag="PPY92_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDJ2626292.1"
                     /translation="MKRLSIIVIIGIFIITGCDWQRTSKEPSKNAQNQQVIKIGYLPI
                     THSANLMMTKKLLSQYNHPKYKLELVKFNNWPDLMDALNSGRIDGASTLIELAMKSKQ
                     KGSNIKAVALGHHEGNVIMGQKGMHLNEFNNGDDYHFGIPHRYSTHYLLLEELRKQLK
                     IKPGHFSYHEMSPAEMPAALSEHRITGYSVAEPFGALGEKLGKGKTLKHGDDVIPDAY
                     CCVLVLRGELLDQHKDVAQAFVQDYKKSGFKMNDRKQSVDIMTHHFKQSRDVLTQSAA
                     WTSYGDLTIKPSGYQEITTLVKQHHLFNPPAYDDFVEPSLYKEASRS"
     gene            2662..3423
                     /locus_tag="PPY92_002316"
     CDS             2662..3423
                     /locus_tag="PPY92_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDJ2626293.1"
                     /translation="MTRPTNNKFILPIITFIIFLGIWEMVIIIGHYQPVLLPGPALVG
                     KSIWTFIVTGEIFQHLAISLWRFVAGFVVALLVAIPLGFLLGRNRWLYNAIEPLFQLI
                     RPISPIAWAPFVVLWFGIGSLPAIAIIFIAAFFPIVFNTIKGVRDIEPQYLKIAANLN
                     LTGWSLYRNILFPGAFKQIMAGIHMAVGTSWIFLVSGEMIGAQSGLGFLIVDARNMLN
                     LEDVLAAIFFIGLFGFIIDRFISYIEQFILRRFGE"
     gene            3436..4467
                     /locus_tag="PPY92_002317"
     CDS             3436..4467
                     /locus_tag="PPY92_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HDJ2626294.1"
                     /translation="MTLETLIKEQLDPHLVEVDEGTYYPRTFIQQLFVDGYFGEAALR
                     KNAEVIEAVSQSCLTTGFCLWCQLAFSTYLENATQPHLNNDLQQQLLSGEILGATGLS
                     NPMKSFNDLEKLNLEHTYVDGQLVVSGRMPAVSNIQEDHYFGAISKHESSDEFVMFIL
                     RANQDGITLVEKTNFLGVNGSATYQITLNQVVVPQSQIITHDAKQFAATIRPQFIAYQ
                     IPIGLGSIKSSLELIDAFSNAQNGINQYLEYDVEAFKKRYRQLREEYYAILDDGNLTS
                     HLSELISLKKDIGYLLLDVNQASVVNGGSRAYTPYSPQVRKLKEGFFFAALTPTLRHL
                     GKLEAELKG"
BASE COUNT         1512 a          693 c          892 g         1415 t
ORIGIN      
        1 atttaaaaaa atcgctcaat atccttgtaa tccgataaga attatagtaa tattttttca
       61 accattgtta taggaggtct tattaatgac attattttta ttagaagcta acaatcttga
      121 ttttgcatca acgaaagaag aactagaagc aaaggcagca tcactatcta cgaagacaat
      181 tccaacatta attgaagtac aagctactga aaatttaact catggttatt ttattgtgga
      241 agcaaatgac gaagcagaag ctaaacaatt tttaacagaa gcagatatta gtattcaatt
      301 agtcaaagaa gtacgcttag ttggtaaaga tttagatgaa gttaaaaatg gtgatgcaca
      361 tgttgattac cttgtaactt ggaacattcc ggaaggcatt acgatggatc aatatttagc
      421 acgtaaaaag aaaaattctg ttcattatga agaagtgcca gaagttgaat ttaaacgcac
      481 atatgtatgt gaagatatgt ctaaatgtat ttgtttatac aacgcacctg atgaagaagc
      541 ggtacgtcgc gcgcgaaaag cagttgatac accgattgat ggcatcgaaa aactttaata
      601 agacaacaag ttgatgagat atatgtatat aggtttgacg tggatttcga ttgcagttaa
      661 ttagaatagc tcaatgctat aaatgtaagt agttgatatg aagaaactaa tgaactaaat
      721 gcaagtattt tctaaaacaa tcattttatt gaaatttagt agagctgaaa ttaatataac
      781 gtcgttaatt gaataacgct tatgttataa gagcactcat accaaaccat aatcatctat
      841 agatataaca attcacgata taagggctgt gtttggcata gccctttaga tatacactta
      901 attcctatta aaatagtagg gattaaaagg gggcttgtca tgattaaaat tcaacaatta
      961 caacatcact ttggatcaca taaagtaatt cataacttta atttggacat tagcaaggga
     1021 gaaatagtca ctttcatagg gaaaagtggt tgcggaaagt ctactttact caatgttatc
     1081 ggtggattta ttcatccatc gtctggtcgt gtcattattg ataacgaaat taaacaacag
     1141 ccatctccag attgtttaat gctatttcaa catcataatt tgctgccatg gaaaacgatt
     1201 aatgacaaca ttaggattgg atttcaacag aaaattagtg atgaagagat taacgcacag
     1261 cttaaattag ttgatttaga agacagggga aagcattttc ccgagcaact gtccgggggt
     1321 atgaaacaac gtgtggcact atgtcgagcg catgtgcata agcctaacgt tatattgatg
     1381 gatgagccat taggtgcatt agatgcattt acacgttata aacttcagga tcaactagtg
     1441 caactaaaac ataaaacgca atcaactatt attttagtga cgcatgacat tgatgaagct
     1501 atttatcttt ccgaccgcat tgttctgtta ggtgaagggt gcaatattat ttctcaatat
     1561 gaaattacag catcacatcc acgcagtcgt aatgatagcc acctacttaa gattcgtaat
     1621 gaaattatgg aaacatttgc attgaatcat catcaagttg aacctgaata ttatttataa
     1681 ggagtgagtg acgatgaaaa ggttaagcat aatcgtcatc attggaatct ttataattac
     1741 aggatgtgat tggcaaagga cgtctaaaga accgtctaaa aatgcccaaa atcagcaagt
     1801 gattaaaatt ggatatttgc cgattacaca ttcagctaat ttgatgatga ctaaaaaatt
     1861 attatcacaa tacaatcatc cgaaatataa actagaatta gttaaattca ataattggcc
     1921 agatttaatg gacgcattaa acagtggtcg tattgatggt gcatcaactt taatagagct
     1981 agcgatgaaa tcaaaacaga agggctcaaa tataaaggct gtggcattgg gccatcatga
     2041 aggcaatgtc attatgggac aaaaaggtat gcacttaaat gaatttaata atggcgatga
     2101 ttaccatttt ggtataccac atcgttattc aacacattat cttttacttg aggaattacg
     2161 taaacaatta aagattaaac cggggcattt tagctatcat gaaatgtcgc cagcagaaat
     2221 gccagccgca ttgagtgaac acagaattac agggtattct gtagccgaac cattcggtgc
     2281 actgggtgaa aagttaggca aaggtaagac tttgaaacat ggtgatgacg ttatacctga
     2341 tgcgtattgc tgtgtgctag tactgagagg ggaattgctt gatcaacaca aggatgtagc
     2401 gcaagcattt gtacaagatt ataaaaagtc tggctttaaa atgaatgatc gcaagcaaag
     2461 tgtagacatt atgacgcatc attttaaaca aagtcgtgac gttttaacac agtcagcggc
     2521 atggacatcc tatggtgatt taacaattaa gccatccggc tatcaagaaa ttacgacatt
     2581 ggtaaaacaa catcatttgt ttaatccacc tgcatatgat gactttgttg aaccgtcatt
     2641 gtataaggag gcatcgcgtt catgacacgt cccacaaata acaaatttat attacctatt
     2701 atcacattta ttattttctt aggcatttgg gaaatggtca ttattattgg gcattaccaa
     2761 cctgtattgt tacctggtcc tgctcttgta ggaaaaagta tatggacttt cattgttact
     2821 ggagaaattt tccaacattt agcaattagt ttatggagat ttgtagcggg ctttgttgtc
     2881 gcattgttgg ttgctattcc attgggcttc ttgcttggaa ggaatcgttg gctatacaac
     2941 gctatcgaac cgctatttca attgattaga ccgatatctc cgatagcatg ggcaccattt
     3001 gttgttctat ggtttggtat tggtagtttg ccagcgattg cgattatttt tatcgctgct
     3061 tttttcccaa ttgtgttcaa tactattaaa ggcgttagag acattgaacc tcaatattta
     3121 aaaatagcgg caaatttaaa tttaactggg tggtcattgt atcgcaatat attatttccc
     3181 ggggcattta aacaaatcat ggctgggata catatggcgg taggaacaag ttggatattt
     3241 ttagtttctg gtgaaatgat tggtgcacaa tcgggattag gttttttaat cgttgatgca
     3301 cgaaatatgt tgaacttaga agatgtttta gcagcaatat tctttatcgg attatttggt
     3361 tttattattg atcgattcat tagttatatt gagcagttta tacttagaag atttggtgaa
     3421 taaggagaga tgatgatgac tttagaaacg cttatcaaag aacaattaga tcctcattta
     3481 gtagaagttg atgaagggac gtattatccg agaacattta ttcaacaatt atttgtagat
     3541 ggttatttcg gtgaggcggc attgagaaaa aatgctgaag taatcgaagc tgtatcgcag
     3601 tcttgtttga caacaggatt ttgtttatgg tgccaattag ctttttcaac gtatttagaa
     3661 aatgccacgc agccacattt aaataatgac ttacaacagc aattgttatc tggagaaata
     3721 ttaggtgcta ccggattgtc taatccgatg aagtcattta atgatttaga aaagttgaac
     3781 cttgaacaca cttatgttga tggacaattg gttgtcagtg gacgtatgcc agctgtaagt
     3841 aatattcaag aagaccatta ttttggtgcg atttcgaaac atgaatcatc agatgaattt
     3901 gtcatgttca ttctacgtgc caatcaagat ggtatcactc tcgttgaaaa aaccaatttt
     3961 ttaggggtca acgggtcagc aacgtatcaa atcacattga atcaagtcgt agtgccacaa
     4021 tcacaaatca tcacgcatga tgcgaagcag tttgcggcaa ctattcgccc gcaatttatt
     4081 gcttaccaaa ttccaatagg attaggctca attaaaagtt ctttagagtt aattgatgca
     4141 ttttcaaatg cgcaaaacgg aataaatcaa tatttagagt atgatgttga agcttttaaa
     4201 aaacgttatc gtcaacttag agaggaatat tatgcaatat tagatgacgg taacttaact
     4261 tcacatttaa gtgaattaat atcattgaag aaggacattg gctatttatt gttagatgta
     4321 aatcaagctt ctgtcgtcaa tggtggttca agagcgtaca caccatattc gccacaagtt
     4381 cgcaagttaa aagaaggatt cttcttcgca gcattaacgc cgacattaag acatttaggt
     4441 aaacttgaag cagagttgaa ggggtaagtg tgataagctg attttttgtt tagatgcgtt
     4501 tgttgaaaca tt
//
LOCUS       DANBWH010000199         4477 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.207, whole genome shotgun sequence.
ACCESSION   DANBWH010000199 DANBWH010000000
VERSION     DANBWH010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.207"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(11..646)
                     /locus_tag="PPY92_002318"
     CDS             complement(11..646)
                     /locus_tag="PPY92_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HDJ2626295.1"
                     /translation="MLGTDELYKLLYRHMGPQNWWPADNDIELMLGAILVQNTRWRNA
                     EIALNQIKEHTHFNPNHILELPIETLQSLIHSSGFYKSKTLTIKTLLTWLARHHFNYQ
                     EINERYKGGLRKELLSLKGIGSETADVLLVYIFGRIEFIPDSYTRKIYDKLGYENTKN
                     YDQLKKVVTLPNHFTNQDANEFHALLDVFGKHYFRDKDIKNYDFLEPYFKK"
     gene            complement(916..2577)
                     /locus_tag="PPY92_002319"
     CDS             complement(916..2577)
                     /locus_tag="PPY92_002319"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDJ2626296.1"
                     /translation="MNIIDQVKQTLVEEIAASINKAGLADEIPDIKIEVPKDTKNGDY
                     ATNIAMVLTKIAKRNPREIAQAIVDNLDTEKAHVKQIDIAGPGFINFYLDNQYLTAII
                     PEAIEKGDQFGHVNESKGQNVLLEYVSANPTGDLHIGHARNAAVGDALANILTAAGYN
                     VTREYYINDAGNQITNLARSIETRFFEALGDNSYSMPEDGYNGKDIIEIGKDLAEKHP
                     EIKDYSEEARLKEFRKLGVEYEMAKLKNDLAEFNTHFDNWFSETSLYEKGEILEVLAK
                     MKELGYTYEADGATWLRTTDFKDDKDRVLIKNDGTYTYFLPDIAYHFDKVKRGNDILI
                     DLFGADHHGYINRLKASLETFGVDSNRLEIQIMQMVRLMENGKEVKMSKRTGNAITLR
                     EIMDEVGVDAARYFLTMRSPDSHFDFDMELAKEQSQDNPVYYAQYAHARICSILKQAK
                     EQGIEVTAANDFTTITNEKAIELLKKVADFEPTIESAAEHRSAHRITNYIQDLAAHFH
                     KFYNAEKVLTDDIEKTKAHVAMIEAVRITLKNALAMVGVSAPESM"
     gene            complement(2574..2990)
                     /locus_tag="PPY92_002320"
     CDS             complement(2574..2990)
                     /locus_tag="PPY92_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDJ2626297.1"
                     /translation="MDKKVSIQTKQVLKQHNEKEKFEFTTEGTWQQRQSNFIRYVEQI
                     EDATVNVTIKVDDDSVKLIRKGDINMNLHFVEGQTTTTFYDISAGRIPLEVKTLRILH
                     FVSGDGGKLKIHYELYQDNEKMGSYQYEINYKEIGE"
     gene            complement(3083..3191)
                     /locus_tag="PPY92_002321"
                     /pseudo
     CDS             complement(3083..3191)
                     /locus_tag="PPY92_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499172.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3266..4276)
                     /gene="adhP"
                     /locus_tag="PPY92_002322"
     CDS             complement(3266..4276)
                     /gene="adhP"
                     /locus_tag="PPY92_002322"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HDJ2626298.1"
                     /translation="MRAAVVTKDHKVSIEDKKLRALKPGEALVQTEYCGVCHTDLHVK
                     NADFGDVTGVTLGHEGIGKVIEVAEDVESLKIGDRVSIAWMFESCGRCEYCTTGRETL
                     CRSVKNAGYTVDGAMAEQVIVTADYAVKVPEKLDPAAASSITCAGVTTYKAVKVSNVK
                     PGQWLGVFGIGGLGNLALQYAKNVMGAKIVAFDINDDKLAFAKELGADAIINSKDVDP
                     VAEVMKLTDNKGLDATVVTSVAKTPFNQAVDVVKAGARVVAVGLPVDKMNLDIPRLVL
                     DGIEVVGSLVGTRQDLREAFEFAAENKVTPKVQLRKLEEINDIFEEMEKGTITGRMVI
                     KF"
BASE COUNT         1374 a          907 c          570 g         1626 t
ORIGIN      
        1 ttcacagcgt ttacttttta aagtaaggtt ctaaaaaatc ataattcttt atatctttgt
       61 ctctaaagta atgtttacca aatacatcta acagagcatg aaattcatta gcatcttgat
      121 ttgtaaaatg atttggtaat gtgactactt tttttaattg atcataattt ttagtgtttt
      181 catatcctaa tttatcatat atttttcttg tatagctatc tggaataaat tcaatacgtc
      241 cgaatatata aacaagtaag acatctgctg tttcacttcc aatacctttc aaagataata
      301 attcttttct taatccacct ttatatcgct cattaatctc ttgataattg aaatgatgtc
      361 gtgctaacca tgttaataat gttttaatcg tcagtgtttt acttttataa aagccacttg
      421 aatgtatcaa tgattgtaac gtttcaatag gtagttctaa tatatgattg ggattaaaat
      481 gcgtatgttc tttaatctga ttcaatgcaa tttctgcatt tcgccatcta gtattttgaa
      541 ctaaaattgc acctaacatc agttcaatgt cattatcagc aggccaccaa ttttgcggtc
      601 ccatatgcct atataacaat ttatacaatt catcagttcc taacatttac accctccttt
      661 tagagcccct aattaataaa ttaattaggg gctcttatgc agttgctgcg caagcaagcc
      721 aactgtattc ctttactccc cttgagcttt agcgatttag gctctcttat gcagttgtgc
      781 agtagcatcc aactgcgtcc ctcttaacct tattcacata ctaagcattc cccaatatgt
      841 cacataccta attaaaaaca ctccaacaca aagtatcttt cgagaaactc tacgttggag
      901 tgtatataaa tgttcttaca ttgattcagg tgcgcttaca ccgaccattg ccaatgcatt
      961 tttcaatgta attctgaccg cttcaatcat agcaacatgt gcttttgttt tttcaatatc
     1021 atctgttaac actttttcag cattatagaa tttatggaaa tgagcagcca agtcttgaat
     1081 atagttagta attctatgtg ctgatctatg ctcagcagca ctttcaattg taggttcgaa
     1141 atcagctact tttttcaaca attcaatcgc tttttcattt gtaatcgttg taaaatcatt
     1201 cgcagcagtc acttcaatac cttgctcttt cgcttgtttt aaaattgaac aaatacgcgc
     1261 atgtgcatat tgagcatagt aaactggatt gtcttgagat tgctctttcg ctaattccat
     1321 atcaaaatca aagtgactat caggactacg catagttaag aaataacgtg cagcgtcaac
     1381 gccaacttcg tccataattt ctcttaatgt aatcgcatta ccagtacgtt tactcatctt
     1441 cacttcttta ccattttcca ttaaacgaac catttgcatg atttgaattt ctaaacgatt
     1501 actatctaca ccaaacgttt caagagatgc tttcaaacga ttaatataac catgatgatc
     1561 agcaccaaat aaatcgatta aaatgtcatt accacgttta actttatcga agtggtacgc
     1621 aatatctggt aagaaatacg tatatgtacc gtcattttta attaatactc tgtctttgtc
     1681 gtctttaaaa tcagttgtac gtaaccatgt agcgccatca gcttcatacg tataacctaa
     1741 ttctttcatt tttgctaaaa cttcaagaat ttcgcctttt tcatataaag atgtttcact
     1801 aaaccaatta tcaaaatgcg tattgaactc tgctaaatca tttttcaatt tagccatttc
     1861 gtattctacg cctaattttc taaattcttt caaacgtgct tcttcagaat aatctttaat
     1921 ttcagggtgt ttctctgcta aatctttacc tatttcaata atatcttttc cattatagcc
     1981 atcttctggc attgaataac tattgtcacc taaagcttca aagaaacgtg tttcaatcga
     2041 acgcgctaag ttagtaattt gattaccagc atcattaata taatattcac gtgttacatt
     2101 atagccagct gcagttaaaa tattagctaa agcatcacca actgctgcat ttctagcatg
     2161 accaatatgt aaatctcctg tagggttagc tgaaacatac tcaagcaata cattttgacc
     2221 ttttgattca tttacatgtc caaattgatc acctttttca attgcttcag gaataattgc
     2281 tgttaaatac tgattatcta agtaaaaatt aatgaatcct ggaccagcaa tgtcaatttg
     2341 ttttacatgt gctttttcag tatctaagtt atcaacaatc gcttgagcaa tttcacgagg
     2401 attacgcttt gcaatcttag tcagtaccat cgcaatatta gtagcataat ctccattttt
     2461 tgtatcttta ggaacttcaa ttttaatatc aggaatctca tctgctaatc ctgctttgtt
     2521 aatacttgct gcaatttctt ctactaatgt ttgtttcact tgatcaataa tattcattcg
     2581 cctatctcct tatagttaat ttcatattga taagaaccca ttttttcatt atcttgatat
     2641 aattcataat gaatctttag cttgccaccg tctccactta cgaaatgtaa aatgcgtaat
     2701 gttttaactt ctagtggaat tcgtccagcc gatatatcgt aaaaagttgt tgtcgtttgt
     2761 ccttcaacaa aatgcaaatt catattaatg tcgcctttac gaatcaactt aacgctatca
     2821 tcatccactt ttattgtaac attaactgtt gcatcctcaa tttgttctac ataccgaata
     2881 aagttagatt gcctttgttg ccaagttcct tcagtagtaa attcaaattt ttctttttcg
     2941 ttgtgctgtt tcaacacttg ctttgtttga atacttactt ttttatccaa tttactactc
     3001 accttaatct tcgcataata gaaatattat agcataaaat aaggtcatct tgttatgcct
     3061 tcttaaagct aaataggttc aattaattaa caattccata cttaatcaac gcttcatata
     3121 tgtaagccat tttataaaat acaaacaaca cctcaaaacc aaaaaaacac gagattttca
     3181 ctatctttca tgaaaacctc gtgtttgatt ataatgatag tgttcagagc actaccttct
     3241 ttatctatat agtcagttga tatttttaaa atttaataac cattctacca gttatagtac
     3301 ccttttccat ttcttcaaaa atatcattga tttcttctaa ttttcttaat tgaactttag
     3361 gtgttacttt attttcagca gcaaattcaa acgcttcacg taagtcttgt cttgtaccaa
     3421 caagtgaacc tactacttca ataccgtcaa gcactaatct tgggatatct aagttcattt
     3481 tatcaacagg taaaccaacg gcaacaactc ttgcaccagc ttttacaaca tcaaccgctt
     3541 ggttaaatgg cgtcttagca actgaagtta ccactgttgc atctaatcct ttgttatcag
     3601 ttaatttcat aacttctgca actggatcaa catctttaga attaataata gcatctgcac
     3661 ctaattcttt cgcgaatgct aatttatcat cattaatgtc gaaggcaaca attttagccc
     3721 ccataacgtt tttagcatat tgtaaagcta ggttacctaa accacctata ccaaaaacac
     3781 ctaaccattg accaggtttt acattactta cttttacagc tttataagtt gtcacacctg
     3841 cgcatgtaat agaagacgct gctgctggat ctaatttttc aggtactttc acagcatagt
     3901 ctgcagtaac aataacttgt tcagccattg caccatctac tgtataacca gcatttttca
     3961 cactacggca aagtgtttca cgacctgttg tacaatattc acatcttcca cagctttcga
     4021 acatccaagc gatagacaca cggtctccaa tttttaatga ttctacatct tctgcaactt
     4081 cgatgacttt accaatacct tcatgaccta aagtaacgcc tgtaacatca ccaaaatcag
     4141 cattcttaac atgtaaatcg gtatgacaaa caccacaata ttccgtttgt accaatgctt
     4201 caccaggttt taaagctctt aactttttgt cctcaatact tactttgtga tctttcgtta
     4261 caactgctgc tctcataata atgtcctcct tatagtcact ccagtatgta tgatattaat
     4321 tggcgattac tttcaaaaat gaaaacgaat aattatagat gtaattttta tttgtaatag
     4381 acgccagatt aaaacatact actgtgactt tgtttttagt tagatatcaa ccgttaactt
     4441 ttaacgtttc cctagttaat atgttaaaag aaatgac
//
LOCUS       DANBWH010000200         4430 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.208, whole genome shotgun sequence.
ACCESSION   DANBWH010000200 DANBWH010000000
VERSION     DANBWH010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.208"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            39..1754
                     /locus_tag="PPY92_002323"
     CDS             39..1754
                     /locus_tag="PPY92_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDJ2626299.1"
                     /translation="MGKLIKYISILLIVVLVLSACGKSSNKDEGVKDATKTETSKHKG
                     GTLNVALTAPPSGVYSSLLNSTHADSVVEGYFNESLLATDKKIRPKAYIASWKDIEPA
                     KKIEFKIKKGIKWHDGNELKIDDWIYSIEVLANKDYEGAYYPSVENIQGAKDYHEGKT
                     DHISGLKKIDDYTMQVTFDKKQENYLTGFITGHLLSKKYLSDVPIKDLAKSDKIRKYP
                     IGIGPYKVKKIVPGEAVQLVKFDDYWQGKPALDKINLKVIDQAQIIKAMEKGDIDVAN
                     DATGAMAKDAKSSNAGLKVLSAPSLDYGLIGFVSHDYDKKANKTGKVRPKYEDKELRK
                     AMLYAIDREKWIKAFFNGYASEINSFVPSMHWIAANPKDLNDYKYDPEKAKKILDKLG
                     YKDRDGDGFREDPKGNKFEINFKHYSGSNPTFEPRTAAIKDFWEKVGLKTNVKLVEFG
                     KYNEDLANASKDMEVYFRSWAGGTDPDPSDLYHTDRPQNEMRTVLPKSDQYLDDALDF
                     EKVGIDEKKRKDIYVKWQKYMNDELPGLPMFQGKSITIVNDKVRNLDIEIGTDQSLYN
                     LTKEA"
     gene            1805..2791
                     /locus_tag="PPY92_002324"
     CDS             1805..2791
                     /locus_tag="PPY92_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDJ2626300.1"
                     /translation="MNNVLLEVKDLETSLKINNEWLATVENISFELSKGEVLGIVGES
                     GCGKSILSKSIIKLLPEKISKLSSGEVIFDGKRIDTLNEKQLLDIRGNDIAMIFQEPM
                     TALNPVFTIKNQLVESIKSHKKISKKEANNLAKDLLKKVGIARQDEILNSYPHQLSGG
                     MRQRVMIAMAISCSPKLLIADEPTTALDVTIQAQILDLLKELQKETQMAIMMITHDLS
                     VVAEFCDKVLVMYAGQIVEFGGIKEILHNPKHPYTQKLLSTIPKLKEGQKRLETIEGI
                     VPSIQAFHVNKCRFANRCNKKLDICNNQSPKMHVCEDVIVRCHLYKNEYKEI"
     gene            2794..3774
                     /locus_tag="PPY92_002325"
     CDS             2794..3774
                     /locus_tag="PPY92_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDJ2626301.1"
                     /translation="MENILEVNQIKKYYKIKTGLLQKTQYVKAVDDVSFSIKKGQTFG
                     LVGESGCGKSTLGKVIIRLEDATSGSIIVNGEDITRLQGKKLRKSRQQYQMIFQDPYA
                     SLNPMQMVGDIISEPILNYKKLPKEEIKKEVLYLLKCVGLSEDAYYKYAHEFSGGQRQ
                     RVGIARALALRPSLIVADEPVSALDVSVQSQVLNLLKDLQEQFKLSYLFIAHDLSVVK
                     HISDVIGVMYLGHIVEIASDKEIYENPKHPYTKALISSIPQIDKHNNNRIILKGELPS
                     PSNPPQGCPFHTRCPIAKDKCKENIPQLKDIGNEHQVACFYVDKVGDLNA"
     gene            3767..>4430
                     /locus_tag="PPY92_002326"
     CDS             3767..>4430
                     /locus_tag="PPY92_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDJ2626302.1"
                     /translation="MLKLILKRLGLMIPLLILISIVVFSLAIIQPGDPFSDLQNGKIK
                     QEAINAQREKLGLNDSIPHQYIRWVNHVIHGDLGESIKYKRPVIDVIEERIPNTILLG
                     AMSLIITYIISFALGITSGRYSYSLTDYTVQIFNYLMLAIPSFIAGVFAIFIFSFELQ
                     WFPFQGSVDINLKEGTFEYYMSKIYHTFLPALTLGLLSTAGYIQYLRNDIIENSKKDY
                     VLT"
BASE COUNT         1707 a          564 c          810 g         1349 t
ORIGIN      
        1 aaaaagaaaa aattagattg aaagtaggag gcatatgtat ggggaagcta attaaatata
       61 tttcaatact tcttattgtc gttttagtgt tgagtgcttg cggaaaaagc agtaataaag
      121 atgaaggagt aaaagatgct actaaaacgg aaacctcaaa acataaaggt ggtaccttaa
      181 atgtagcatt aacagcaccg ccaagtggtg tttattcttc gttattaaat agtacacatg
      241 cagattctgt agttgaggga tattttaacg aaagcttatt agcaactgat aaaaaaatac
      301 gtcctaaggc atatattgct tcatggaagg acatcgagcc ggctaagaaa atagaattta
      361 aaattaaaaa aggtattaaa tggcatgatg gtaatgaatt gaaaattgat gattggattt
      421 attcaattga agtcttagct aacaaggact acgaaggtgc ttattatcca agtgtagaaa
      481 atatccaagg tgcgaaagat tatcatgaag gaaaaactga tcatattagc ggattgaaga
      541 aaatagatga ctacactatg caggttacat ttgataaaaa acaagaaaat tacttaacag
      601 gatttattac tggacattta ttaagtaaaa aatatttatc agatgtacca attaaagatt
      661 tagcgaaatc agataaaatc cgaaaatatc ctattggtat tggaccgtat aaagttaaga
      721 aaatcgttcc aggtgaggct gttcaactcg ttaaatttga tgattattgg caaggtaagc
      781 ctgcactaga caaaatcaat ttaaaagtta ttgatcaagc tcaaattatt aaggcaatgg
      841 aaaaaggcga tattgatgtt gcgaatgatg ctaccggtgc aatggcaaaa gatgctaagt
      901 catctaatgc tggtctcaag gtattatctg cgccaagctt agactacggt ttaataggat
      961 tcgtatctca tgattacgat aaaaaagcta ataaaactgg taaagtgaga ccaaaatatg
     1021 aagacaaaga attacgtaaa gcaatgcttt atgcaattga tagagaaaaa tggattaaag
     1081 cgtttttcaa tggttacgct agtgaaatca atagttttgt accatctatg cattggatag
     1141 cagccaatcc taaggaccta aatgattaca aatatgatcc tgaaaaagct aaaaaaatct
     1201 tagataagtt aggttataaa gatagagatg gtgacggatt tagagaagat cctaaaggta
     1261 ataaatttga gattaacttt aaacattatt caggttcaaa tcctactttt gaaccaagaa
     1321 ctgctgcgat aaaagatttc tgggaaaaag ttggcttgaa aacaaatgtg aagttagtag
     1381 aattcggtaa atataatgaa gacttagcaa atgcatctaa agatatggaa gtgtacttca
     1441 gatcatgggc aggaggtaca gatccagatc catcagattt ataccacact gatagacctc
     1501 aaaatgaaat gagaacagtt ttaccaaaat cagatcaata tttagatgat gcattagact
     1561 tcgaaaaagt aggcattgat gaaaagaaac gtaaagatat ttatgttaaa tggcaaaaat
     1621 atatgaatga tgagttacct ggattaccaa tgttccaagg taaatcgata actattgtta
     1681 acgataaagt acgaaactta gacattgaaa ttggaactga tcaaagttta tataatttaa
     1741 ctaaagaagc ttagtaatgt aacctcgacg attttgtcga ggtttactta tatgggggga
     1801 tgttatgaat aatgtattgt tagaggttaa agatttagaa acatcattaa aaataaataa
     1861 tgaatggtta gcaactgttg aaaatatttc ttttgaatta tctaaaggag aagttttggg
     1921 tatagtaggg gaatctggtt gcggtaagtc catattaagt aagtcaatta ttaaattatt
     1981 accagaaaag atatctaaac taagtagtgg agaagttata tttgatggta aacgaatcga
     2041 tacgctcaat gagaagcaat tgctagatat tcgaggaaat gatattgcta tgatttttca
     2101 agaacctatg actgctttaa atcctgtatt taccataaaa aatcaacttg tggaatctat
     2161 aaaatcacat aaaaaaattt ctaaaaaaga agcaaataac ttagcaaaag atttactaaa
     2221 aaaagttgga attgctagac aagatgaaat attaaatagc tatcctcatc aattatctgg
     2281 tggtatgaga caaagagtaa tgattgcaat ggccatttca tgttctccta aattattaat
     2341 tgctgatgaa cctacaacag cattggatgt cacgattcaa gcgcaaatat tagacttatt
     2401 aaaagaattg caaaaggaaa cgcaaatggc aattatgatg attacacatg atttgagtgt
     2461 agttgctgag ttttgcgata aagtcttagt tatgtatgca ggtcaaattg tagaatttgg
     2521 aggcataaaa gaaatactac acaatccgaa acatccttat acccaaaaat tattatcaac
     2581 aattccaaaa cttaaagaag ggcagaaacg acttgaaacg atagaaggaa ttgtgccatc
     2641 aatccaagca tttcacgtta ataagtgcag atttgcaaat agatgtaaca aaaaactgga
     2701 tatttgtaat aatcaatctc ctaaaatgca tgtttgtgaa gacgtcattg tacgttgtca
     2761 tttgtacaaa aatgaatata aggagatata agaatggaaa atattttaga agtcaaccaa
     2821 ataaaaaaat actacaaaat taaaactgga ttattacaaa aaactcagta cgttaaagct
     2881 gttgatgacg tatcgttttc aataaaaaaa ggacaaactt ttggattagt aggagaatcg
     2941 ggttgtggta agtcaacgtt aggtaaagtg attatcaggc ttgaagatgc aacttcaggt
     3001 tcaataattg ttaatggtga agatataaca agattacaag gtaaaaaact cagaaaatca
     3061 cgacaacaat atcagatgat atttcaagat ccgtatgcat cattgaatcc gatgcaaatg
     3121 gttggagata tcatttcaga acctatttta aattataaaa aattgccaaa agaagaaata
     3181 aaaaaagaag tactatattt attaaaatgt gttggcctaa gtgaagatgc atattataaa
     3241 tatgcacatg aattttcagg tggacagaga caaagagtgg gaattgcaag agcattggct
     3301 ttgcgtccga gtttaattgt tgctgatgag cctgtaagtg cattagatgt atctgttcaa
     3361 tctcaagtac tgaatttatt aaaagattta caagagcaat ttaagttaag ctatttattt
     3421 atcgcgcatg atttaagtgt agtgaaacat attagtgatg taattggagt tatgtattta
     3481 ggtcatatag ttgaaattgc atctgataaa gaaatttatg aaaatcccaa acatccatat
     3541 acaaaagcat taatttcatc aataccacaa attgataaac ataataacaa tagaattata
     3601 ttaaaaggag aattaccttc gccaagtaat ccgccgcaag gttgtccttt tcatacaaga
     3661 tgtccgattg caaaagataa atgtaaagaa aatataccac aattaaagga cattggtaac
     3721 gaacatcaag ttgcttgttt ctacgtagat aaagtaggtg atttaaatgc ttaaattaat
     3781 acttaaaagg ttagggttaa tgatcccgtt actaatttta atctcaatag ttgtattttc
     3841 attagcaatc atacaaccag gagatccatt ttcagattta caaaacggta aaataaaaca
     3901 agaagcaata aatgcacaaa gggaaaagtt aggcctcaac gactcaatac cacatcaata
     3961 tattagatgg gtcaatcatg ttatacatgg tgatttaggg gagtcaatca aatataagag
     4021 gccggtaatt gatgttattg aggaaagaat tccaaataca atattactcg gtgctatgtc
     4081 attaattatt acttatatta tctcatttgc tttaggaata acgtcaggta gatattctta
     4141 cagtttgact gattatactg tgcaaatatt taattatttg atgttagcta ttccatcttt
     4201 tattgcggga gtatttgcaa tttttatttt ttcttttgaa ttacaatggt ttccgtttca
     4261 aggttctgtt gatattaacc ttaaagaagg tacttttgaa tattatatga gtaaaattta
     4321 tcatacattt ttgcctgcat taactttagg attattatct actgctggtt atattcaata
     4381 tttacgtaat gatattattg aaaattctaa aaaagattat gtattgacgg
//
LOCUS       DANBWH010000201         4427 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.209, whole genome shotgun sequence.
ACCESSION   DANBWH010000201 DANBWH010000000
VERSION     DANBWH010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.209"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            367..2556
                     /locus_tag="PPY92_002327"
     CDS             367..2556
                     /locus_tag="PPY92_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDJ2626303.1"
                     /translation="MNNEYPYSADEVLHKAKSYLSADEYEYVLKSYHIAYEAHKGQFR
                     KNGLPYIMHPIQVAGILTEMRLDGPTIVAGFLHDVIEDTPYTFEDVKEMFNEEVARIV
                     DGVTKLKKVKYRSKEEQQAENHRKLFIAIAKDVRVILVKLADRLHNMRTLKAMPREKQ
                     IRISRETLEIYAPLAHRLGINTIKWELEDTALRYIDNVQYFRIVNLMKKKRSEREAYI
                     ETAIDRIRTEMDRMNIEGDINGRPKHIYSIYRKMMKQKKQFDQIFDLLAIRVIVNSIN
                     DCYAILGLVHTLWKPMPGRFKDYIAMPKQNLYQSLHTTVVGPNGDPLEIQIRTFDMHE
                     IAEHGVAAHWAYKEGKKVSEKDQTYQNKLNWLKELAEADHTSSDAQEFMETLKYDLQS
                     DKVYAFTPASDVIELPYGAVPIDFAYAIHSEVGNKMIGAKVNGKIVPIDYILQTGDIV
                     EIRTSKHSYGPSRDWLKIVKSSSAKGKIKSFFKKQDRSSNIEKGRMMVEVEIKEQGFR
                     VEDILTEKNIQVVNEKYNFANEDDLFAAVGFGGVTSLQIVNKLTERQRILDKQRALNE
                     AQEVTKSLPIKDNIITDSGVYVEGLENVLIKLSKCCNPIPGDDIVGYITKGHGIKVHR
                     TDCPNIKNETERLINVEWVKSKDATQKYQVDLEVTAYDRNGLLNEVLQAVSSTAGNLI
                     KVSGRSDIDKNAIINISVMVKNVNDVYRVVEKIKQLGDVYTVTRVWN"
     gene            2568..3020
                     /locus_tag="PPY92_002328"
     CDS             2568..3020
                     /locus_tag="PPY92_002328"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDJ2626304.1"
                     /translation="MKVVVQRVKEASVTNDTLNNQIKKGYCLLVGIGQNSTEQDADVI
                     AKKIANARLFEDDNNKLNFNIQQMNGEILSVSQFTLYADVKKGNRPGFSNSKNPDQAV
                     KIYEYFNDALRAYGLTVKTGEFGTHMNVSINNDGPVTIIYESQDGKIQ"
     gene            3017..3892
                     /locus_tag="PPY92_002329"
     CDS             3017..3892
                     /locus_tag="PPY92_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDJ2626305.1"
                     /translation="MKKIEAWLSKKGLKNKRTLIVVIAFVLFIIFLFLLLNSNSEDSG
                     NITITENAELRTGPNAAYSVIYKVEKGDHFKKIGKVGKWIEVEDTSSNEKGWIAGWHT
                     NLDIVADNTKEKNPLQGKTIVLDPGHGGSDQGASSNTKYKSLEKDYTLKTAKELQRTL
                     EKEGATVKMTRTDDTYVSLENRDIKGDAYLSIHNDALESSNANGMTVYWYHDNQRALA
                     DTLDATIQKKGLLSNRGSRQENYQVLRQTKVPAVLLELGYISNPTDETMIKDQLHRQI
                     LEQAIVDGLKIYFSA"
BASE COUNT         1655 a          574 c          882 g         1316 t
ORIGIN      
        1 ttaaaaaagt tgtttaagtt ttcttaacat gagatgttag gattttttat ttactgaaaa
       61 tgttagatga ttgagcatta taccttaata acatcgttta tttatttcat aaattgtagt
      121 atcatagaac taatatttaa aaaatgaaac agtagattta ggtcgaattt ttgtaaaagt
      181 tttaaaagta ggaatagtat acaaattaaa ctcgctcaag taaaattaat attacgatta
      241 atgacgacag gataaatatt tatcgtcgac ggacgtatga ttggtgtggg acaaatacta
      301 ttcaacaaga gtacctaaat cattgtttaa ggcgaagtaa taaatatgaa tggggtgtat
      361 catataatga acaacgaata tccatatagt gcagatgaag ttcttcacaa agcaaaatca
      421 tatttgtcag cagatgaata tgagtatgtt ttaaaaagct atcatattgc ttatgaagca
      481 cataaaggtc agttccgaaa aaacggatta ccatacatta tgcatcctat acaagttgca
      541 ggtattttaa cagaaatgcg attagacgga ccgacgattg tcgcaggttt tttgcatgat
      601 gtaattgaag atacaccgta tacatttgaa gatgtaaaag aaatgttcaa tgaagaagtt
      661 gctcgaattg ttgatggtgt gacgaagctt aaaaaagtaa aataccgctc aaaagaagaa
      721 caacaagctg aaaatcatcg caagttattt attgcgattg ccaaagatgt acgcgtaatt
      781 ttggtgaaat tagcagacag attacataat atgcgtacct tgaaagccat gccgcgtgaa
      841 aaacaaatta gaatttctcg agaaacatta gaaatttatg cacctttagc acatcgtctt
      901 ggtattaata caattaaatg ggaactagaa gatacggctc ttcgttatat tgataatgtg
      961 caatatttta gaatagtcaa tttaatgaag aagaaacgta gtgaacgtga agcgtatatc
     1021 gaaacggcta ttgatagaat acgtactgaa atggaccgaa tgaatatcga aggcgatata
     1081 aatggtagac ctaaacatat ttacagtatt tatcggaaaa tgatgaagca gaaaaaacaa
     1141 tttgatcaaa tttttgattt gttggcgata cgtgttattg tcaattctat taatgattgt
     1201 tatgcgatac ttgggttggt gcatacgtta tggaaaccga tgccaggacg ttttaaagat
     1261 tatattgcaa tgcctaaaca aaatttgtat cagtcattgc atactacagt agtaggtcca
     1321 aatggagacc cgctcgaaat ccaaatacga acgtttgata tgcacgaaat tgctgagcat
     1381 ggtgttgcag cacactgggc ttacaaagaa ggtaaaaaag taagtgaaaa agatcaaact
     1441 tatcaaaata agttaaattg gttaaaagaa ttagctgaag cggatcatac atcgtctgac
     1501 gctcaagaat ttatggaaac cttaaaatat gacttacaga gtgacaaagt atacgcattt
     1561 accccagcga gtgatgttat tgagttgcca tatggtgctg tgccgattga ttttgcttat
     1621 gcgattcaca gtgaagtagg taataagatg attggtgcca aggtgaatgg caaaattgta
     1681 ccaattgact atattttaca aacaggcgat attgttgaaa tacgtactag taaacattca
     1741 tatggaccaa gtcgtgattg gttgaaaatt gttaaatcgt ctagtgccaa aggtaaaatt
     1801 aaaagtttct tcaaaaaaca agatcgttca tctaatattg aaaaaggccg aatgatggtt
     1861 gaagttgaaa taaaagagca aggatttaga gtcgaagata ttttgacaga gaaaaatatt
     1921 caggttgtta atgaaaaata taactttgca aatgaagatg atttattcgc agctgtagga
     1981 tttggcggcg tgacatcctt acagattgtt aataaattaa ctgaaagaca acgtatttta
     2041 gataaacaac gtgctttaaa tgaagcacaa gaagttacga aatcattgcc tattaaagac
     2101 aacatcatta ctgatagtgg tgtctatgta gaaggtttag aaaatgtact tatcaagttg
     2161 tcaaaatgtt gtaatcctat accaggtgat gatattgtag gttatatcac caaaggtcac
     2221 ggtattaaag tacatcgcac tgattgccca aatattaaga acgaaactga acgactaatt
     2281 aatgttgaat gggtaaaatc aaaagacgca actcaaaaat atcaggttga tttagaggta
     2341 actgcgtatg accgaaatgg cttgttgaat gaagtactac aagctgttag ctcgacagcc
     2401 ggcaatttaa ttaaagtttc aggacgttca gatattgata aaaatgcaat aataaatatt
     2461 agtgtcatgg tgaaaaacgt gaatgatgtt tatcgtgtgg tagaaaagat caaacaactt
     2521 ggtgatgttt atacagtaac aagagtttgg aactagaggt gcaaaatatg aaagtagttg
     2581 tacaaagagt taaagaagca tcggtgacga atgatacatt aaataatcaa atcaaaaaag
     2641 gatattgttt attagtcggt atcggtcaga actctacaga gcaagatgca gatgtaattg
     2701 caaagaaaat tgctaatgca agattatttg aagatgacaa taataaatta aactttaata
     2761 tccaacaaat gaatggtgaa atactatcag tttcacaatt tactctctat gcagatgtaa
     2821 aaaaaggtaa ccgtccaggt ttctcaaatt ctaaaaatcc tgatcaagcg gtaaaaattt
     2881 atgagtattt taatgatgcg ctacgagcgt atggtcttac tgtgaaaaca ggtgaatttg
     2941 gaacacacat gaatgttagc ataaataatg atggtccagt cactattatt tatgaaagtc
     3001 aggacggcaa aattcaatga aaaaaataga ggcatggtta tctaaaaagg gtcttaaaaa
     3061 taaacgtact ctaatagtag tgattgcctt tgtcttattt atcatctttt tatttttatt
     3121 gctgaatagc aatagtgaag atagtgggaa catcacgata actgaaaatg ctgaattacg
     3181 tacaggtcca aacgctgcgt attcagtcat atataaagtt gagaaaggtg accattttaa
     3241 aaagattggt aaagtaggta aatggattga agttgaagat acatccagta atgaaaaagg
     3301 ttggatagct ggatggcaca caaatttaga tattgtcgcg gataatacga aggagaaaaa
     3361 tcctttgcaa ggtaaaacaa tagtgcttga tcctggtcat ggaggtagtg accagggtgc
     3421 ttcaagcaat actaaatata aaagtttaga aaaagactat acgttgaaaa cagcaaaaga
     3481 attgcagcgt actttagaaa aagaaggcgc aactgttaag atgacaagaa cagacgatac
     3541 atatgtttca ctagaaaatc gtgatatcaa aggcgatgcc tatttgagta tacataatga
     3601 tgcattagaa tcatctaatg caaatggaat gacagtttat tggtatcatg ataatcaaag
     3661 agctttagca gatacgttag acgctacgat tcagaagaaa ggtttacttt ctaatcgcgg
     3721 ttcaagacaa gaaaattatc aagtgttaag acaaacaaaa gttcctgctg ttttattaga
     3781 attaggttat attagtaacc caactgatga aacgatgatt aaagatcaat tacatagaca
     3841 aattttagaa caagcaattg ttgatggact taaaatttat ttttctgcgt agggcttgca
     3901 aaaatatgtg aaagtagtta tcattgatat tgaattttat aactaaaacc gttagtattc
     3961 ttgaaatggt aaatgaaata ggtagcaatc taactaagat tgtgtaggaa tataatccat
     4021 agactgaaag attatgctga gtagtttata tacattgaac acaagaagag gtgctttatg
     4081 aaaagtaaag ccgttaaacg tacgttaaac gttttgagtg ggtttattta atgcacgctt
     4141 ataaaaagta atgatgatta caattaggca tgttttttaa accaatgagg gtggcaacac
     4201 ggatattcgt cccttgtgca gtttcaggct gtgcaagggc tttttctttt gcttttaaaa
     4261 ttatgattta tcatgcaaag ttaagtgatc gtatatagtt ttagttttaa aaaggtaatt
     4321 aaataaaata gtttgccgag ggagatgtca aaatgattaa aatacctaga gggacgcagg
     4381 atattttacc tgaagattca aagaaatggc gttacattga aaatcaa
//
LOCUS       DANBWH010000202         4355 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.210, whole genome shotgun sequence.
ACCESSION   DANBWH010000202 DANBWH010000000
VERSION     DANBWH010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.210"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            60..1001
                     /gene="pde2"
                     /locus_tag="PPY92_002330"
     CDS             60..1001
                     /gene="pde2"
                     /locus_tag="PPY92_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pApA hydrolase Pde2"
                     /protein_id="HDJ2626306.1"
                     /translation="MISTLNEIMKCIEDNDTIIIHRHVRPDPDAYGSQLGLKYYIQQK
                     FPQKQVFAVGEAESSLSFIGELDNIDDKTYQDALVIVCDTANAPRIDDERYSTGSKLI
                     KIDHHPAVDQYGDINLVNTNASSTSEIIYDLISHFNDEAIVNKDIASVLYLGIVGDTG
                     RFLFNNTSEHTMEIAGKLIGHDIDHNALLNKMMEKDPKMLPFQGYVLQHFELMDDGFC
                     QVKITEDVLEQFGIQPNEASQFVNTIADIKGLKIWVFAVDEGSEIRCRLRSKGQLIIN
                     DIAQDFGGGGHPNASGVSVNSWDEFEQLATALRTKLN"
     gene            1022..4219
                     /locus_tag="PPY92_002331"
     CDS             1022..4219
                     /locus_tag="PPY92_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDJ2626307.1"
                     /translation="MVAYLNIHTAYDLLNSSLKIEDAVRLAVSENVDALAITDTNVLY
                     GFPKFYDACIANNIKPIFGMTIYVTNGLNTVETVVLAKNNDGLKDLYQLSSEIKMNSM
                     ENVSFELLQQFSSNLIIIFKNVADEHRDIVQVFDSHEDTYLDHQSVLVQGIKHVWIQN
                     VCYQTRQDADTISVLAAIRDNAKLDLIHDQEDFGAHFLTEKEIKQLDINQEYLTQVDV
                     IAQKCNAELKYHQSLLPQYQTPNDESAKKYLWRVLVTQLKKLELNYDVYLERLKYEYK
                     VITNMGFEDYFLIVSDLIHYAKTNDVMVGPGRGSSAGSLVSYLLGITTIDPIKFNLLF
                     ERFLNPERVTMPDIDIDFEDTRRERVIQYVQEKYGELHVSGIVTFGHLLARAVARDVG
                     RIMGFDEVTLNEISSLIPHKLGITLDEAYQIDDFKKFVHRNHRHERWFSICKKLEGLP
                     RHTSTHAAGIIINDHPLYEYAPLTKGDTGLLTQWTMTEAERIGLLKIDFLGLRNLSII
                     HQILIQVKKDLGINIDIEKIPFDDQKVFELLSQGDTTGIFQLESDGVRSVLKKLKPEH
                     FEDIVAVTSLYRPGPMEEIPTYITRRHDPSKVQYLHPHLEPILKNTYGVIIYQEQIMQ
                     IASTFANFSYGEADILRRAMSKKNRAVLESERQHFIEGAKQNGYHEDISKQIFDLILK
                     FADYGFPRAHAVSYSKIAYIMSFLKVHYPNYFYANILSNVIGSEKKTAQMIEEAKKQG
                     ITILPPNINESHWFYKPSQEGIYLSIGTIKGVGYQSVKVIVDERYQNGKFKDFFDFAR
                     RIPKRVKTRKLLEALILVGAFDAFGKTRSTLLQAIDQVLDGDLNIEQDGFLFDILTPK
                     QMYEDKEELPDALISQYEKEYLGFYVSQHPVDKKFVAKQYLTIFKLSNAQNYKPILVQ
                     FDKVKQIRTKNGQNMAFVTLNDGIETLDGVIFPNQFKKYEELLSHNDLFIVSGKFDHR
                     KQQRQLIINEIQTLATFEEQKLAFAKQIIIRNKSQIDMFEEMIKATKENANDVVLSFY
                     DETIKQMTTLGYINQKDSMFNNFIQSFNPSDIRLI"
BASE COUNT         1559 a          591 c          803 g         1402 t
ORIGIN      
        1 tattttgata tagactaatg cttaaattaa gcaacttttc taaaaaaggg agtatgaaaa
       61 tgattagtac tttgaatgaa attatgaaat gtatcgaaga caacgataca attattatac
      121 acagacatgt tagaccagat ccagatgcat atggttcaca acttggttta aaatactata
      181 ttcagcagaa atttccgcaa aagcaagtat ttgcagtggg tgaagcggaa tcatcattaa
      241 gttttattgg agagttggat aacattgatg ataaaacata tcaagatgcg cttgtaattg
      301 tatgtgatac tgccaatgct ccacgaattg acgatgaacg atatagtaca ggtagtaaac
      361 ttattaaaat agatcatcat cctgcagttg atcagtatgg tgatattaat ttagttaata
      421 cgaacgcgtc atctacaagt gaaatcattt atgatttaat ctcacatttt aatgatgaag
      481 caattgttaa taaagatatc gcgagtgttt tataccttgg tatcgtcggt gatactgggc
      541 gattcctttt taacaatacc tcagaacata ctatggaaat tgctggaaag ttaattgggc
      601 atgatattga tcataatgcg ctattaaata aaatgatgga gaaggaccca aaaatgttgc
      661 cgtttcaagg ttatgtttta caacatttcg aacttatgga tgatggattc tgccaagtta
      721 aaataactga agatgtattg gagcaattcg gtattcagcc aaatgaagca tctcagtttg
      781 ttaatacaat tgctgacatc aaaggtttga aaatatgggt gtttgcagtt gatgagggta
      841 gtgaaataag atgtcgctta cgctctaaag ggcaattgat tattaatgat attgcgcaag
      901 attttggtgg cggtggccat ccgaatgcgt caggagtttc agtgaacagc tgggatgaat
      961 ttgagcaact tgctacagct ttacgcacaa aacttaacta atagaaagga gccattcaat
     1021 catggtggca tatttaaata ttcatacggc ttatgatttg ttaaattcaa gcttaaaaat
     1081 agaagatgcc gtaagacttg ctgtgtctga aaatgttgat gcacttgcca taactgacac
     1141 caatgtattg tatggttttc ctaaatttta tgatgcatgt atagcaaata acattaaacc
     1201 gatttttggt atgacaatat atgtgacaaa tggattaaat acagtcgaaa cagttgttct
     1261 agctaaaaat aatgatggat taaaagattt gtatcaacta tcatcggaaa taaaaatgaa
     1321 ttcaatggaa aatgtttcat ttgaactatt gcaacaattt tcatcgaatt tgattatcat
     1381 ttttaaaaat gttgcagacg aacatcgtga cattgttcaa gtttttgatt cgcatgaaga
     1441 tacgtattta gatcatcaaa gtgttttagt tcagggtata aagcacgtat ggattcaaaa
     1501 tgtttgttac caaacacgtc aagatgccga tacgatttct gtattagcag ctattagaga
     1561 caatgcaaaa ttagacttaa ttcatgatca agaagatttt ggtgcacatt ttttaactga
     1621 aaaggaaatt aaacaattag atattaacca agaatattta acgcaggttg atgttatagc
     1681 tcaaaagtgt aatgcagaat taaaatatca tcaatctcta cttcctcaat atcagacacc
     1741 taacgatgaa tcagctaaaa aatatttgtg gcgtgtctta gttacacaat tgaaaaaatt
     1801 agaacttaat tatgacgtct atttagagcg attgaaatat gagtataaag ttattactaa
     1861 tatgggtttt gaagattatt tcttaatagt aagtgattta atccattatg cgaaaacgaa
     1921 tgatgtgatg gtaggtccag gtcgtggttc ttcagctggc tcactggtca gttatttact
     1981 aggtattaca acgattgatc ctattaaatt caatctatta tttgaacgtt ttttaaaccc
     2041 agaacgtgta acaatgcctg atatagatat tgactttgaa gatacacgcc gagaaagggt
     2101 tattcagtac gtccaagaaa aatatggcga gctacatgta tctggaattg tgactttcgg
     2161 tcatctgctt gcaagagcgg ttgctaggga tgttggacga attatggggt ttgatgaagt
     2221 tacattaaat gaaatttcaa gtttaatccc acataaatta ggaattacac ttgatgaagc
     2281 atatcaaatt gacgatttta aaaaatttgt acatcgaaac catcgacatg aacgctggtt
     2341 cagtatttgt aaaaagttag aaggtttacc aagacataca tctacacatg cggcaggaat
     2401 tattattaat gaccatccat tatatgaata tgccccttta acgaaagggg atacaggatt
     2461 attaacgcaa tggacaatga ctgaagccga acgaattggg ttattaaaaa tagattttct
     2521 agggttgaga aacttatcga ttattcatca aatcttaata caagtcaaaa aagatttagg
     2581 tattaatatt gatatcgaaa agattccgtt cgatgatcaa aaagtgtttg aattgttgtc
     2641 gcaaggagat acgactggca tattccaatt agagtcagac ggtgtaagaa gtgtattaaa
     2701 aaaattaaag ccggaacact ttgaagatat tgttgctgta acttctttgt atagaccagg
     2761 tccaatggaa gaaattccaa cttacattac aagaagacat gatccaagca aagttcaata
     2821 tttacatcca catttagaac ctatattaaa aaatacttac ggtgttatta tttatcaaga
     2881 gcaaattatg caaatagcga gcacatttgc aaacttcagt tatggtgaag cggatatttt
     2941 aagaagagca atgagtaaaa aaaatagagc tgttcttgaa agtgagcgtc aacattttat
     3001 agaaggtgca aagcaaaatg gttatcacga agacattagt aagcaaatat ttgatttgat
     3061 tctgaaattt gctgattatg gttttcctag agcacatgct gtcagctatt ctaaaattgc
     3121 atacattatg agctttttaa aagtccatta tccaaattat ttttacgcaa atattttaag
     3181 taatgttatt ggaagtgaga agaaaactgc tcaaatgata gaagaagcaa aaaaacaagg
     3241 tatcactata ttgccaccga acattaacga aagtcattgg ttttataaac cttcccaaga
     3301 aggcatttat ttatcaattg gtacaattaa aggtgttggt tatcaaagtg tgaaagtgat
     3361 tgttgatgaa cgttatcaga acggcaaatt taaagatttc tttgattttg ctagacgtat
     3421 accgaagaga gtcaaaacga gaaagttact tgaagcactg attttagtgg gagcgtttga
     3481 tgcttttggt aaaacacgtt caacgttgtt gcaagctatt gatcaagtgt tggatggcga
     3541 tttaaacatt gaacaagatg gttttttatt tgatatttta acgccaaaac agatgtatga
     3601 agataaagaa gaattgcctg atgcacttat tagtcagtac gaaaaagaat atttaggatt
     3661 ttatgtttcg caacacccag tagataaaaa gtttgttgcc aaacaatatt taacgatatt
     3721 taaattgagt aacgcgcaga attataaacc tatattagta cagtttgata aagttaaaca
     3781 aattcgaact aaaaatggtc aaaatatggc attcgtcaca ttaaatgatg gcattgaaac
     3841 tttagatggt gtgattttcc ctaatcagtt taaaaagtac gaagagttgt tatcacataa
     3901 tgacttgttt atagttagcg ggaaatttga ccatagaaag caacaacgtc aactaattat
     3961 aaatgagatt cagacattag ccacttttga agaacaaaaa ttagcatttg ccaaacaaat
     4021 tataattaga aataaatcac aaatagatat gtttgaagag atgattaaag ctacgaaaga
     4081 gaatgctaat gatgttgtgt tatcctttta tgatgaaacg attaaacaaa tgactacttt
     4141 aggctatatt aatcaaaaag atagtatgtt taataatttt atacaatcct ttaaccctag
     4201 tgatattagg cttatataac ttttatcaac taataaatta tgatatagta aactgatggt
     4261 tagatatttt ttaaccatca gtttgcgttt atattagttt tttatgctta ttatttttat
     4321 gagtttcact ttacattatt gattaatcca taaga
//
LOCUS       DANBWH010000203         4341 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.211, whole genome shotgun sequence.
ACCESSION   DANBWH010000203 DANBWH010000000
VERSION     DANBWH010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.211"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(487..592)
                     /locus_tag="PPY92_002332"
                     /pseudo
     CDS             complement(487..592)
                     /locus_tag="PPY92_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(797..1393)
                     /gene="recR"
                     /locus_tag="PPY92_002333"
     CDS             complement(797..1393)
                     /gene="recR"
                     /locus_tag="PPY92_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDJ2626308.1"
                     /translation="MHYPEPISKLIDSFMKLPGIGPKTAQRLAFHTLDMKEDDVVQFA
                     KALVDVKRELTYCSVCGHITENDPCYICEDKQRDRSVICVVEDDKDVIAMEKMREYKG
                     LYHVLHGSISPMDGIGPEDINIPSLIERLKSDEVSELILAMNPNLEGESTAMYISRLV
                     KPIGIKVTRLAQGLSVGGDLEYADEVTLSKAIAGRTEM"
     gene            complement(1400..1717)
                     /locus_tag="PPY92_002334"
     CDS             complement(1400..1717)
                     /locus_tag="PPY92_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002479279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDJ2626309.1"
                     /translation="MRGGGNMQQMMKQMQKMQKKMAQEQEKLKEERIVGTAGGGMVAV
                     TVTGHKEVVDVEIKEEAVDPDDIEMLQDLVLAATNEAMNKADELTQERLGKHTQGLNI
                     PGM"
     gene            complement(1807..3504)
                     /gene="dnaX"
                     /locus_tag="PPY92_002335"
     CDS             complement(1807..3504)
                     /gene="dnaX"
                     /locus_tag="PPY92_002335"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDJ2626310.1"
                     /translation="MNYQALYRMYRPQSFEDVVGQEHVTKTLRNAISKEKQSHAYIFS
                     GPRGTGKTSIAKVFAKAINCLNSTDGEPCNECHICKGITQGTNSDVIEIDAASNNGVD
                     EIRNIRDKVKYAPSESKYKVYIIDEVHMLTTGAFNALLKTLEEPPAHAIFILATTEPH
                     KIPPTIISRAQRFDFKAISLDQIVERLKFVADAQQIECEDEALAFIAKASEGGMRDAL
                     SIMDQAIAFGDGTLTLQDALNVTGSVHDEALDHLFDDIVQGDVQASFKKYHQFITEGK
                     EVNRLINDMIYFVRDTIMNKTSEKDTEYRALMNLELDMLYQMIDLINDTLVSIRFSVN
                     QNVHFEVLLVKLAEQIKGQPQVIANVAEPAQIASSPNTDVLLQRMEQLEQELKTLKAQ
                     GVSVAPAQKSSKKPARGIQKSKNAFSMQQIAKVLDKANKADIKLLKDHWQEVIDHAKN
                     NDKKSLVSLLQNSEPVAASEDHVLVKFEEEIHCEIVNKDDEKRSSIESVVCNIVNKNV
                     KVVGVPSDQWQRVRTEYLQNRKNEGDDMPKQQAQQTDIAQKAKDLFGEETVHVIDEE"
     gene            complement(3573..4097)
                     /locus_tag="PPY92_002336"
     CDS             complement(3573..4097)
                     /locus_tag="PPY92_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDJ2626311.1"
                     /translation="MQIYLSTLTELDYDKSLNSIEESFDDNPETSWQARAKVKHLRKS
                     PCYNFELEVIAKNENNDVVGHVLLIEVEINSDDKTYYGLAIASLSVHPELRGQKLGRG
                     LVQAVEERAKAQEYSTVVVDHCFDYFEKLGYQNAAEHDIKLESGDAPLLVKYLWDNLT
                     DAPHGIVKFPEHFY"
     gene            complement(4235..4336)
                     /locus_tag="PPY92_002337"
     CDS             complement(4235..4336)
                     /locus_tag="PPY92_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626312.1"
                     /translation="MKTIQRIIRGTCLWEVAFLYVKFDSSELDVQFE"
BASE COUNT         1322 a          931 c          566 g         1522 t
ORIGIN      
        1 ggaattaacg ttgacatatt gtcattcagt tttcaatgtt cattttttct taccgacaag
       61 aattaattat acattttatt aacatttaag tcaacaactt tttaaaatta attttcgtta
      121 gtaattcaag ttatttctta agttgagttg ttttgtttgc ctcaacaaat aaatactata
      181 caggctttca aactgcttaa caacagtaaa aattacacct ttgtcatctg tactgaacgc
      241 ccttatccta aaaatcgaac ttaaaaaatc cccacttata aatagtacgt tttttagaac
      301 aagaaacaga tttttctcta accatatcaa tcaaaaaaac cgcaaaacag aacgttattt
      361 ttttaccaga aattaaaaat aaatttacaa gttttaaata atctctcaaa acaccgctat
      421 acaggctgat ttaataagct ttagaccaaa gaagtacaac tactaagcca gcatctatca
      481 tttcaattac aaaagaattt catttgcata gtcattaacc atcaaccgca tttatcaaat
      541 taatagcacc aacacaccat aaccgtaaat ctaattcgat aatccaattc acatcattac
      601 tattagaagt aacaaatcac aaccaacggc ttgcttccta aagattaccc atccctcagt
      661 aaaactaggt aaaccatacc accgccacac cgcaaataaa gtagtacatc acaccacacc
      721 ataaaaaaag ccacaatgat tacactgtga cttttcttac tatagtatta aattcaaaaa
      781 tgtttaatag aagacattac atttctgttc tacctgcgat tgctttagat aatgttactt
      841 cgtcagcata ctctaaatcg ccacctaccg ataacccttg tgctaatctc gtcactttga
      901 tacctatagg cttaactaat ctagaaatat acatggctgt agattccccc tctaagttcg
      961 ggttcatagc taagattaat tcgctaactt catcgctttt caagcgttca atcaatgaag
     1021 gaatattaat atcttctggt ccaatgccat ccataggtga aatagaccca tgtaaaacgt
     1081 gatataaacc tttgtattct ctcatttttt ccatagctat gacatctttg tcatcttcca
     1141 caacacaaat aactgaacga tctctttgtt tatcttcaca aatataacat ggatcatttt
     1201 cagtaatgtg accacatacg ctacaatatg ttaattctct cttaacatct actaatgctt
     1261 tggcaaactg aacaacatcg tcttctttca tatctaaggt atgaaaagcc agacgttggg
     1321 ctgtctttgg accaatgcct ggcaatttca taaagctatc aataagcttt gatataggtt
     1381 ctggataatg catctatgat cacattccag ggatgtttaa gccttgagta tgtttaccta
     1441 aacgttcttg agtaagctca tcagctttat tcatcgcttc attagtagct gctaacacta
     1501 agtcttgtag catttcaata tcgtctgggt ctacagcttc ttctttgatt tcaacgtcga
     1561 caacttcttt atgaccagtt acagtaactg caaccatgcc accgccagct gttcctacaa
     1621 tacgctcttc tttaagtttt tcttgttctt gagccatttt cttttgcatt ttttgcattt
     1681 gtttcatcat ttgttgcatg tttccgccac cgcgcatatt ccatttcctc cttgaaaatc
     1741 aataaatatt tatcaataaa atgatgtttc tttcattata catacgatta tatcgcttgt
     1801 catgtatcac tcttcatcta tcacatgtac agtttcttca ccgaaaagat cttttgcttt
     1861 ttgagcaata tctgtttgtt gtgcttgttg ctttggcata tcatcgcctt cgtttttacg
     1921 attttgtaaa tactccgttc gaactctttg ccattgatct gatggtacac caacaacttt
     1981 aacgttttta ttaacgatat tacatacaac actttctata ctactacgtt tctcgtcgtc
     2041 tttattgacg atttcacaat ggatctcttc ctcaaatttc acaagtacgt gatcttcact
     2101 tgccgccaca ggttccgaat tttgcaataa actaacgagt gattttttat cattgttttt
     2161 ggcatgatca atcacttctt gccaatgatc tttcaacaat ttgatatctg ccttattcgc
     2221 tttatctagc acttttgcaa tttgttgcat tgaaaatgca tttttagatt tttgtatgcc
     2281 tctcgcaggc tttttcgaag atttttgagc aggagcgaca ctcactcctt gtgcttttag
     2341 tgtttttaat tcttgctcta actgttccat acgttgcaac aatacatctg tgtttggcga
     2401 tgaagcaatt tgtgctggtt cagctacatt cgcaatcact tgtggttgac ccttaatctg
     2461 ctcagctaat tttactaaca acacttcaaa atgaacgttt tgattcacac taaaacgaat
     2521 cgacactaat gtatcattaa taagatcaat catttgatat aacatatcta attctaagtt
     2581 catcagtgct cgatactcag tatctttctc agatgtttta ttcataatcg tatctctgac
     2641 aaaataaatc atatcattta ttaggcgatt cacttcttta ccctctgtta taaactgatg
     2701 gtatttttta aaagatgctt gtacgtcacc ttgtacaata tcatcaaaca agtgatccaa
     2761 cgcttcatca tgtacgctac ctgtgacatt caacgcatct tgcaatgtta acgtaccatc
     2821 accaaatgca atagcctgat ccataatact taatgcatca cgcatacccc cttcagacgc
     2881 tttagcgata aatgccaagg cttcatcttc acattcaatt tgttgtgcat ctgctacaaa
     2941 ttttaaacgt tcaacaattt gatctaggct aattgcttta aaatcaaaac gttgtgccct
     3001 agaaatgatt gttggaggga ttttatgtgg ttctgtcgtt gccaatataa aaatagcgtg
     3061 tgctggaggt tcttctaacg tctttaaaag ggcattaaaa gcacctgttg ttagcatgtg
     3121 cacctcatct ataatataaa ctttatattt cgattcactt ggtgcatatt taactttgtc
     3181 tctaatattt cttatttcat caacgccatt attactagca gcatcaattt ctatcacatc
     3241 tgaattagtc ccctgcgtaa tgcctttaca aatatgacat tcattacaag gttctccatc
     3301 agtgctattt agacagttga ttgctttagc aaacactttg gcaatactcg ttttccccgt
     3361 acctctcgga ccactaaaaa tataagcatg cgactgtttt tctttcgaaa tcgcattgcg
     3421 caatgtcttc gtgacatgtt cttgtccgac gacatcctcg aaactttggg gtctgtacat
     3481 acgatataag gcttgataat tcaagttagc acctccataa acaattacct ctcattatag
     3541 catgataata cctttacttc ttaattgaac aattaataaa aatgttctgg aaattttacg
     3601 attccgtgtg gtgcatccgt caaattatcc cataaatatt ttacaagtaa cggtgcatca
     3661 ccagattcta atttaatgtc atgctcagca gcattttgat aacccaactt ttcaaagtag
     3721 tcaaaacaat ggtctacaac aaccgtacta tactcttgtg ctttggcacg ctcttctact
     3781 gcttgaacca agccacgacc taatttttgt ccacgtaatt caggatgaac tgataaagag
     3841 gcaatcgcca aaccataata cgtcttatca tcactattaa tttctacttc aattaataaa
     3901 acgtgtccaa cgacatcgtt attttcattt ttcgctatta cttctaattc aaaattatag
     3961 caaggagatt ttcttaaatg ttttactttc gcacgtgctt gccaactcgt ttcaggatta
     4021 tcatcaaaac tttcttcaat actatttaaa gatttatcat aatctaactc tgttaaagta
     4081 cttaaatata tttgcatatg tcctccgtag gcatttgatt gtcaataatc ataacgtatc
     4141 tcattaataa ttctattgta agatactccc cctcatttca ccatcatttc tttatcatca
     4201 aattataact tcttactttt cattgacaca aaaatcattc aaactgcaca tcgagttcac
     4261 ttgaatcaaa cttcacatat aaaaaagcta cttcccacaa acatgttcca cgtataatac
     4321 gctgaattgt cttcaagaaa g
//
LOCUS       DANBWH010000204         4283 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.212, whole genome shotgun sequence.
ACCESSION   DANBWH010000204 DANBWH010000000
VERSION     DANBWH010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.212"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            89..772
                     /locus_tag="PPY92_002338"
     CDS             89..772
                     /locus_tag="PPY92_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDJ2626313.1"
                     /translation="MEWILFDKDGTLIEFDRSWEKIGVRFVQSLLETFPVHNKEAALR
                     QLGVIKESIDPKSVMGSGSLQQIIQAFNDVTGQDTTDWSKSTSQKLVDERIPEINWVE
                     GVKEALIDLKAKGYQLGIVTSDTKKGVEQFLAHTNAISLFDLIISTEADAYEKPNPKV
                     LSPLFEQYNVDPQKVAIVGDTANDMKTASNANLGMAIGVLTGIATKEELHEADIILNS
                     AADILEALN"
     gene            complement(888..1550)
                     /locus_tag="PPY92_002339"
     CDS             complement(888..1550)
                     /locus_tag="PPY92_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDJ2626314.1"
                     /translation="MNNYGIPQNAIITIAGTVGVGKSTLTQALADKLNFKTSFENVEH
                     NPYLDKFYSDFERWSFHLQIYFLAERFKEQKRMFEYGGGFVQDRSIYEDVDIFAKMHE
                     EEGTMSKEDFKTYSDLFNAMVMTPYFPKPDVMIYLECNYDEVIDRIIERGREMEINTD
                     PEYWKKLFKRYDDWINSFNACPVVRININEYDIHKDPESLNPMIDKIARIIQTYRQVD
                     TR"
     gene            complement(1543..2160)
                     /locus_tag="PPY92_002340"
     CDS             complement(1543..2160)
                     /locus_tag="PPY92_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDJ2626315.1"
                     /translation="MNKPFIAIEGPIGVGKSSLAHKLSQTLDFYEEKEIITENPFLSD
                     FYEDISKWSFQTEMFFLCNRYKQFQDVTQLNQGVVSDYHIHKNKIFAKNTLSPVEFQK
                     FSKIYDILTEDMIMPNMIIFLDADLDVLKSRIAKRNRSFEHQIEDEYLLKLKKDYREY
                     YESLQSNGSNVVLIDTTSIDFLKNEQDYEDILHIILPMIGDITNE"
     gene            2227..2697
                     /locus_tag="PPY92_002341"
     CDS             2227..2697
                     /locus_tag="PPY92_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HDJ2626316.1"
                     /translation="MTNDIYFMTLAIEEAKKAAQLGEVPIGAIITKDDEVIARAHNLR
                     ETLQQPTAHAEHIAIERAAKVLGSWRLEGCTLYVTLEPCVMCAGTIVMSRIPRVVYGA
                     DDPKGGCSGSLMNLLQQSNFNHRAIVDKGVLKEACSTLLTTFFKNLRANKKSTN"
     gene            2844..3713
                     /locus_tag="PPY92_002342"
     CDS             2844..3713
                     /locus_tag="PPY92_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDJ2626317.1"
                     /translation="MIKLIATDMDGTLLNAAHEISQPNIDAIKYAQEQGITVVIATGR
                     AFYEAQAPVADTDLTVPYICLNGAEVRDETFNVMSTSHLNKSLVHKITNVLKDAGIYY
                     QVYTSRAIYTEDPQRDLDIYIDIAERAGQHANVERIKNGIQRRIDNGTLKVVDNYDAI
                     ENIPGELIMKILAFDGNLEKIDKASKILAESPNLAISSSSRGNIEITHSDAQKGIALE
                     TIAERLGIEMKDVMAIGDNLNDLSMLEKVGYPVAMENGAEEVKKIAKYVTDTNENSGV
                     GKAIMKLLREQQV"
     gene            3735..>4283
                     /locus_tag="PPY92_002343"
     CDS             3735..>4283
                     /locus_tag="PPY92_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDJ2626318.1"
                     /translation="MKGLIIIGSAQVNSHTSALARYLTEHFKTHDIEAEIFDLAEKPL
                     NQLDFSGTTPSIDEIKQNMKDLKEKAMAADFLILGTPNYHGSYSGILKNALDHLNMDY
                     FKMKPVGLIGNSGGIVSSEPLSHLRVIVRSLLGIAVPTQIATHDSDFAKNEDGSYYLN
                     DSEFQLRARLFVDQIVSFVNNSP"
BASE COUNT         1559 a          613 c          752 g         1359 t
ORIGIN      
        1 gaacaatact acgagttcat tttcagggga taacgaatat catagaatca gcataatacg
       61 aaataaataa ataggagtat tgatatcaat ggaatggata ttatttgata aagatggtac
      121 gttaattgaa tttgatagaa gttgggaaaa aataggggta cgatttgtac aatcattgct
      181 tgagactttc ccagtacata ataaagaagc tgctttaaga caactcggtg tcattaaaga
      241 atctattgat ccaaaatcag tgatgggttc aggatcttta caacaaatta tccaggcatt
      301 taatgatgtg acgggacaag atacaaccga ctggtccaag tcaacaagtc aaaagctggt
      361 agatgaacgt attcctgaaa ttaattgggt agaaggtgtt aaagaagcac ttatcgattt
      421 gaaagcaaaa ggctatcaac ttggtattgt tacgagtgat actaaaaaag gtgtagaaca
      481 atttttagca cataccaatg ctatctcgtt gttcgatttg atcatttcta ccgaagcgga
      541 tgcctatgag aagccaaatc ctaaagtatt atcgccttta tttgagcaat ataatgtaga
      601 tcctcagaaa gtagctatag taggagacac tgctaatgat atgaagacag caagtaatgc
      661 aaatttaggt atggcaatag gtgtattaac aggtattgca acaaaagaag aattacatga
      721 agctgatatt attttaaata gtgcggcaga tattttagaa gctttaaatt aaaaagaaag
      781 acatagtgta tatgtgaatt aatgtattcg atagaattga taatgtaatc gatgatatct
      841 atggaatata cattgaatat aaacatatac gctatgtctt tagtctttta tcgtgtatct
      901 acttgtcgat atgtttgaat aattcgagca attttgtcta tcataggatt taaagattcg
      961 gggtccttat ggatatcata ttcattaata ttgatacgta caactggaca tgcattaaag
     1021 ctattaatcc aatcgtcata gcgtttaaat agctttttcc agtattcagg gtctgtatta
     1081 atttccattt cgcgaccacg ttcaataata cgatcaatga cctcatcata gttacattct
     1141 aaataaatca ttacatctgg tttaggaaaa taaggtgtca tgaccatggc attaaataag
     1201 tctgaatatg ttttgaaatc ttctttactc attgtgcctt cttcttcatg catttttgca
     1261 aaaatatcaa catcttcata aattgatcga tcttggacaa agccaccacc atattcaaac
     1321 atacgctttt gttctttaaa acgttcagct aagaagtaaa tttgcaaatg gaaactccat
     1381 cgttcaaaat cgctataaaa tttatctaaa tatggattat gttcgacatt ttcaaaagac
     1441 gttttaaagt ttaatttatc tgcaagtgct tgcgttagtg ttgattttcc aacaccaact
     1501 gtacctgcaa tggttataat ggcattttgt ggaataccgt aattattcat tggtaatatc
     1561 tcctatcata ggtaatataa tatgtaatat atcttcgtaa tcttgttcat ttttaagaaa
     1621 atcaatagaa gttgtatcga ttaaaactac atttgaacca ttactttgta aggactcata
     1681 atactcacga taatcttttt ttaactttaa cagatattca tcttctattt gatgctcaaa
     1741 actacggtta cgtttagcaa ttctagattt taacacatca aggtctgcat ctaaaaagat
     1801 aatcatattc ggcataatca tatcttcagt taaaatatca taaattttac tgaatttctg
     1861 aaattcaaca ggactcaaag tatttttagc aaatatctta tttttatgta tatgataatc
     1921 actaactaca ccttgattta gttgtgttac atcttgaaat tgcttatatc tattgcataa
     1981 aaagaacatt tcagtttgaa aactccattt agagatatct tcataaaagt ctgataaaaa
     2041 tggattttct gtgatgattt ctttttcttc ataaaaatct aaagtttgac ttaatttgtg
     2101 tgcaagtgaa gatttaccta cgccaatagg accttcaatt gctataaaag gtttgttcat
     2161 attcacatct ccaaatgtta aaatagacat tgagtattgt accataagtg aggtatctgg
     2221 acataaatga caaatgatat atattttatg acattagcga ttgaagaagc taaaaaagca
     2281 gctcaactag gcgaagtacc tataggtgct atcatcacta aagatgatga agttatcgct
     2341 agagcacata atttaagaga aacactacaa caaccaacgg cgcatgctga acatattgca
     2401 attgaacgtg cagccaaagt gttaggtagt tggcgtttag aaggttgcac attatatgta
     2461 accttagaac catgtgtcat gtgcgcagga acaattgtaa tgagtcgcat tccaagagtc
     2521 gtctatggcg cagatgatcc taaaggtggt tgtagtggca gtttaatgaa tttattgcaa
     2581 caatctaatt ttaatcatcg tgcaattgtt gataaaggtg tacttaaaga agcatgtagc
     2641 acattattaa caacattttt taaaaactta agagccaata agaaatccac caattagacg
     2701 atagtacata ctaattaaaa tttgttagaa ttacattata tgataaatta atgacaatat
     2761 aaatgttaat agaaaagtat gtgcgctgag tatatattct tatttaagaa caagatttta
     2821 aatttacgaa acgaggtaac ataatgataa aactaatagc cactgatatg gatggcacgc
     2881 tacttaatgc agcacatgaa atttctcaac ctaatattga tgcgattaaa tacgctcaag
     2941 aacaagggat aacggttgtt atcgcgacag gtcgagcatt ttatgaagca caagcaccag
     3001 ttgctgacac agatttaaca gtaccatata tttgtttgaa tggtgctgaa gtacgtgatg
     3061 aaactttcaa tgtaatgagc acttcacacc ttaataaatc gttagtacac aaaattacaa
     3121 atgttttaaa agatgcaggt atttattatc aagtatacac gagtcgtgcg atttatactg
     3181 aagatccaca aagagattta gacatttaca tagatattgc tgagcgtgca ggtcaacatg
     3241 caaacgttga gcgtattaaa aatggtattc aaagacgcat agataatggt acgttgaaag
     3301 ttgttgataa ttatgatgct attgaaaaca tacctggtga attaattatg aaaatattag
     3361 catttgatgg aaatttagaa aaaattgaca aagctagtaa aattttagct gaatctccga
     3421 atttagctat atcatcatct tcgagaggaa atatagaaat aacgcattca gatgcacaaa
     3481 aaggtattgc gctagaaaca attgccgaaa gattagggat tgaaatgaaa gatgtcatgg
     3541 caataggtga caatttaaat gacttatcaa tgttagagaa agttggctat ccagttgcga
     3601 tggaaaatgg tgcagaagaa gttaaaaaaa tagcgaaata tgtcacagat acgaatgaaa
     3661 atagtggtgt tggaaaagct attatgaaat tattacgtga acaacaagtt taataaaaaa
     3721 aagaggggtc aaatatgaaa ggattaatta ttattggcag tgcacaagtg aattcacata
     3781 caagtgcact agcaagatac ttaactgagc attttaaaac acatgatatt gaagcggaaa
     3841 tattcgattt agcagaaaaa ccgttaaatc aattagattt ttcaggaaca acaccgtcta
     3901 ttgatgaaat caaacaaaat atgaaagatt taaaagagaa agcaatggcg gcggactttt
     3961 taatattagg aacgccaaac tatcatggtt catattctgg aatattgaaa aatgcattag
     4021 atcatctaaa tatggattat tttaaaatga aacctgtagg cttaatagga aatagtggtg
     4081 gtattgttag ttcagagcca ttgtcacatt taagagtaat cgtcagaagt ttactaggca
     4141 ttgctgtacc aactcaaata gcaacacatg attctgattt tgctaaaaat gaagatggtt
     4201 catattactt aaatgatagt gaattccaat tacgagcaag attatttgtc gatcaaattg
     4261 tatcttttgt gaataatagt cca
//
LOCUS       DANBWH010000205         4276 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.213, whole genome shotgun sequence.
ACCESSION   DANBWH010000205 DANBWH010000000
VERSION     DANBWH010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.213"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            43..4122
                     /gene="sdrD"
                     /locus_tag="PPY92_002344"
     CDS             43..4122
                     /gene="sdrD"
                     /locus_tag="PPY92_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrD"
                     /protein_id="HDJ2626319.1"
                     /translation="MLNRENKTAITRKGMVSNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAESTNKELNEATTSASDNQSSDKVDMQQLNQEDNTKNDNQKEMVSSQGNE
                     TTSNGNKSIEKESVQSTTGNKVEVSTAKSDEQASPKSTNEDLNTKQTISNQEGLQPDL
                     LENKSVVNVQPTNEENKKVDAKTESTTLNVKSDAIKSNAETLVDNNSNSNNENNADII
                     LPKSTAPKSLNTRMRMAAIQPNSTDSKNVNDLITSNTTLTVVDADNSKTIVPAQDYLS
                     LKSQITVDDKVKSGDYFTIKYSDTVQVYGLNPEDIKNIGDIKDPNNGETIATAKHDTA
                     NNLITYTFTDYVDRFNSVKMGINYSIYMDADTIPVDKKDVPFSVTIGNQITTTTADIT
                     YPAYKEADNNSIGSAFTETVSHVGNVEDPGYYNQVVYVNPMDKDLKGAKLKVEAYHPK
                     YPTNIGQINQNVTNIKIYRVPEGYTLNKGYDVNTNDLVDVTDEFKNKMTYGSNQSVNL
                     DFGDITSAYVVMVNTKFQYTNSESPTLVQMATLSSTGNKSVSTGNALGFTNNQSGGAG
                     QEVYKIGNYVWEDTNKNGVQELGEKGVGNVTVTVFDNNTNTKVGEAVTKEDGSYLIPN
                     LPNGDYRVEFSNLPKGYEVTPSKQGNNEELDSNGLSSVITVNGKDNLSADLGIYKPKY
                     NLGDYVWEDTNKNGIQDQDEKGISGVTVTLKDENGNVLKTVTTDADGKYKFTDLDNGN
                     YKVEFTTPEGYTPTTVTSGSDIEKDSNGLTTTGVINGADNMTLDSGFYKTPKYNLGNY
                     VWEDTNKDGKQDSTEKGISGVTVTLKNENGEVLQTTKTDKDGKYQFTGLENGTYKVEF
                     ETPSGYTPTQVGSGTDEGIDSNGTSTTGVIKDKDNDTIDSGFYKPTYNLGDYVWEDTN
                     KNGVQDKDEKGISGVTVTLKDENDKVLKTVTTDENGKYQFTDLNNGTYKVEFETPSGY
                     TPTSVTSGNDTEKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQ
                     DSTEKGIKDVKVILLNEKGEVIGTTKTDENGKYRFDNLDSGKYKVIFEKPTGLTQTGT
                     NTTEDDKDADGGEVDVTITDHDDFTLDNGYYEEETSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDAGKHTPVKPMSTTKDHHNKAK
                     ALPETGNENSGSNNATLFGGLFAALGSLLLFGRRKKQNK"
BASE COUNT         1727 a          643 c          773 g         1133 t
ORIGIN      
        1 caattgtatt atttaaaaat ttgatgaatt aggagtaatc taatgctaaa cagagaaaat
       61 aaaacggcaa taacaagaaa aggcatggta tccaatcgat taaataaatt ttcgattaga
      121 aagtacacag tgggaacagc atcaatttta gtaggtacaa cattaatttt tggtctgggg
      181 aaccaagaag caaaggctgc agaaagtact aataaagaat tgaacgaagc gacaacttca
      241 gcaagtgata atcaatcgag tgataaagtt gatatgcagc aactaaatca agaagacaat
      301 actaaaaatg ataatcaaaa agaaatggta tcatctcaag gtaatgaaac gacttcaaat
      361 gggaataaat caatagaaaa agaaagtgta caatctacca ctggaaataa agttgaagtt
      421 tcaactgcca aatcagatga gcaagcttca ccaaaatcta cgaatgaaga tttaaacact
      481 aaacaaacta taagtaatca agaagggtta caacctgatt tgctagagaa taaatcagtg
      541 gtaaatgttc aaccaactaa tgaggaaaac aaaaaggtag atgcgaaaac tgaatcaact
      601 acattaaatg ttaaaagtga tgctatcaag agtaatgctg aaactcttgt tgataacaat
      661 agtaattcaa ataatgaaaa taatgcagat atcattttgc caaaaagtac agcacctaaa
      721 agtttgaata caagaatgcg tatggcagca atacaaccaa actcaacaga ttctaaaaat
      781 gttaatgatt taatcacatc aaatacaaca ttaactgtcg ttgatgcaga taatagcaaa
      841 acgattgtac cagcccaaga ttatttatca ttaaaatcac aaattacagt tgatgacaaa
      901 gttaaatcag gtgattattt cacaattaaa tactcagata cagtacaagt atatggattg
      961 aatccggaag atattaaaaa tattggtgat attaaagatc caaataatgg tgaaacaatt
     1021 gcgactgcaa aacatgatac tgcaaataat ttaattacat atacatttac agattatgtt
     1081 gatcgattta attcagtaaa aatgggtatt aattactcaa tttatatgga tgcagataca
     1141 attcctgttg acaagaaaga tgttcctttt agtgtaacta ttggaaatca aattacaact
     1201 acaacagcag atatcactta tccggcttat aaagaagctg acaataattc aataggatca
     1261 gcttttacag agacagtttc tcatgtagga aatgttgaag accctggtta ctataaccag
     1321 gtagtatatg ttaatcctat ggataaggat ttaaaaggtg ctaagttaaa agttgaagcg
     1381 taccatccga aatatccaac taatattggt caaattaatc aaaatgttac aaatataaaa
     1441 atatatcgtg ttcctgaagg atatacattg aataaaggat atgacgttaa tactaatgat
     1501 ttggtagacg taactgatga atttaaaaat aaaatgacgt atggatcaaa tcaaagtgtt
     1561 aatcttgatt ttggtgatat tacatcagca tatgttgtaa tggttaatac aaaattccaa
     1621 tatacaaata gcgaaagccc aacacttgtt caaatggcta ctttatcttc aacaggtaat
     1681 aaatccgttt ctactggcaa tgctttagga tttactaata accaaagtgg cggagctggt
     1741 caagaagtat ataaaattgg taactacgta tgggaagata ctaataaaaa cggtgttcaa
     1801 gaattaggag aaaaaggcgt tggcaatgta actgtaactg tatttgataa taatacaaat
     1861 acaaaagtag gagaagcagt tactaaagaa gatgggtcat acttgattcc aaacttacct
     1921 aatggagatt accgtgtaga attttcaaac ttaccaaaag gttatgaagt aaccccttca
     1981 aaacaaggta ataacgaaga attagattca aacggcttat cttcagttat tacagttaat
     2041 ggcaaagata acttatctgc agacttaggt atttacaaac ctaaatacaa cttaggtgac
     2101 tatgtctggg aagatacaaa taaaaatggt atccaagacc aagatgaaaa aggtatatct
     2161 ggcgtaacgg taacattaaa agatgaaaac ggtaacgtgt taaaaacagt tacaacagac
     2221 gcagatggca aatataaatt tactgattta gataatggta attataaagt tgaatttact
     2281 acaccagaag gctatacacc gactacagta acatctggta gcgacattga aaaagactct
     2341 aatggtttaa caacaacagg tgttattaat ggtgctgata acatgacatt agatagtgga
     2401 ttctacaaaa caccaaaata taatttaggt aattatgtat gggaagatac aaataaagat
     2461 ggtaagcagg attcaactga aaaaggtatt tcaggcgtaa cagttacatt gaaaaatgaa
     2521 aacggtgaag ttttacaaac aactaaaaca gataaagatg gtaaatatca atttactgga
     2581 ttagaaaatg gaacttataa agttgaattc gaaacaccat caggttacac accaacacaa
     2641 gtaggttcag gaactgatga aggtatagat tcaaatggta catcaacaac aggtgtcatt
     2701 aaagataaag ataacgatac tattgactct ggtttctaca aaccgactta caacttaggt
     2761 gactatgtat gggaagatac aaataaaaac ggtgttcaag ataaagatga aaagggtatt
     2821 tcaggtgtaa cagttacgtt aaaagatgaa aacgacaaag ttttaaaaac agttacaaca
     2881 gatgaaaatg gtaaatatca attcactgat ttaaacaatg gaacttataa agttgaattc
     2941 gagacaccat caggttatac accaacttca gtaacttctg gaaatgatac tgaaaaagat
     3001 tctaatggtt taacaacaac aggtgtcatt aaagatgcag ataacatgac attagacagt
     3061 ggtttctata aaacaccaaa atatagttta ggtgattatg tttggtacga cagtaataaa
     3121 gacggcaaac aagattcaac tgaaaaaggt atcaaagatg ttaaagttat tttattaaat
     3181 gaaaaaggcg aagtaattgg aacaactaaa acagatgaaa atggtaaata ccgctttgat
     3241 aatttagata gcggtaaata caaagttatt tttgaaaagc ctactggctt aacacaaaca
     3301 ggtacaaata caactgaaga tgataaagat gccgatggtg gcgaagttga tgtaacaatt
     3361 acggatcatg atgatttcac acttgataat ggctactacg aagaagaaac atcagatagc
     3421 gactcagatt cggacagcga ttcagactca gacagcgatt cagactcaga tagtgattca
     3481 gattcagata gtgattcaga ttcagatagt gattcagatt cagacagcga ctcagactca
     3541 gatagtgact cagactcaga tagcgattca gattcagata gcgattcaga ctcagacagc
     3601 gattcagatt cagacagcga ctcagactca gatagcgact cagattcgga cagcgattca
     3661 gactcagata gcgactcaga ctcagacagc gattcagact cagatagcga ctcagactca
     3721 gatagcgatt cagattcaga cagcgattca gattcagaca gtgattcaga ttcagacagt
     3781 gattcagatt cagatagcga ctcagactca gatagcgact cagattcaga cagtgattca
     3841 gattcagata gcgactcaga ctcagatagc gactcagatt cagacagcga ttcagactca
     3901 gatagcgact cagattcaga cagtgattca gactcagatg caggtaagca cacacctgtt
     3961 aaaccaatga gtactactaa agaccatcac aataaagcaa aagcattacc agaaacaggt
     4021 aatgaaaata gtggctcaaa taacgcaacg ttatttggcg gattattcgc agcattagga
     4081 tcattattgt tattcggtcg tcgtaaaaaa caaaataaat aatacagctt ataccaggtc
     4141 cgtgaggcct ggttttttat ttcaaaattt atgaaatgta tcttatgttt gagattaaat
     4201 agtgcaattt tttaaaaaat ataacataaa tcaacataaa tttaattcat ctttacatta
     4261 aaagtacaaa cgaata
//
LOCUS       DANBWH010000206         4268 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.214, whole genome shotgun sequence.
ACCESSION   DANBWH010000206 DANBWH010000000
VERSION     DANBWH010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.214"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..381
                     /locus_tag="PPY92_002345"
     CDS             <1..381
                     /locus_tag="PPY92_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020807974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HDJ2626320.1"
                     /translation="INKTQNSGYENEYFYIVANIPTLQEYRKYYEPLIKKNNLNFKKG
                     MKQARKGVGYKAAIEVHTTLFSRSSNFSKDKKLDDVLDLSESTKKLHLNFENTKIFLQ
                     LAKSTISTNRVNYSDNESIRIEVE"
     gene            439..1353
                     /locus_tag="PPY92_002346"
     CDS             439..1353
                     /locus_tag="PPY92_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HDJ2626321.1"
                     /translation="MFKRIKLILIATIILSGCSTTNNESNNETKSVPEEMEASKYVGQ
                     GFQLPAEKDAIEFAKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGVEVYVHCEDH
                     GIVFNASLPLYKDAIHQKGSMRSNDNGDDMSMMVGTVLSGFEYRAQKEKYDNLYKFFK
                     ENEKKYQYTGFTKEAINKTQNVGYQNEYFYITYLSRNLKEYRKYYEPLIHKNDKEFKE
                     GMQRARKELDYTANSNTVATLFSTNDKKNRKEKINNVIDLSEKIERTKDMPIKNTITT
                     QLGNKLIGTKKARFDDKKVVSFGAFEDE"
     gene            1346..3373
                     /locus_tag="PPY92_002347"
     CDS             1346..3373
                     /locus_tag="PPY92_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626322.1"
                     /translation="MNKINDRDLTELSSYWVYQDINKDNDFTVNGKRFKQVDEYNDNG
                     NKNKKGASDLKIYELLDEKGKPTGEQTMIYQGTSNEAINPNNPLKSLDIGDDWLQNAK
                     LMDNSNKSTDYLKQSDEFADLYRDKLNDANKLSKYNFTQKYGVSPNNYKNKTIVADGG
                     NSEGGAGAKYQGAKHPNEKVVATDPAMVPYAAWQKFARPRFDNMISFNSTNDLLTWLQ
                     DPFIKDMPGKRVNISDGVPRLDALIDSHVGYKRKLNRKDNTYDTVPLIKIKSVKDTEI
                     KNGKKVKKTINITLDMDGRIPINVWTGDSIARSGRGTLIKLNLENLDALSKLITGETS
                     GMLAECVIFLNESFNISENENKNFADRKQQLSEGFKDKINLFQLEEMERTLISKINSL
                     EEVADETIESISAVKHLLPDFALDALKERINELFKGIKSFIEKVYDSIDNEILEIFKN
                     IDHDFRDGVSEEMMKHLKVVKQNIERIKNQNDIYGRQIAEIRSIMKQQDATILDGNFQ
                     INCSGENMVQGLVIPSNYLGRKMKILKDHIDDGIKKIADYVQSIYDEYASKIVDVIKY
                     LINTIPKIRKNLRHAIEMLNVKKKEFLSLIPNVTCNYIKTKLEELDNTLGKWEPFLND
                     LKAVSPILDNHLDDIVKNMKPLIVQMLFEPSHYDDMFISRKALTPVFSSVL"
     gene            complement(3416..>4268)
                     /locus_tag="PPY92_002348"
     CDS             complement(3416..>4268)
                     /locus_tag="PPY92_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDJ2626323.1"
                     /translation="TKYMQIFINKLSDIYSQNQVTRQLGDMKGAIKYAVKFYNYPNEY
                     LLTNVKIPKRRKTIEDIEKDESKMYNYLEMNQVLQIRDHILNDNKLHKRNRILIASIL
                     EVQALTGMRIGELQALQEKDIDLLNKTINITGTIHRIKYEEGFGYKDTTKTISSKRSI
                     SINSRTVEIFKKIILENKMLKRWNSSYVDRGFIFTTKKGNPLCNNQIAGVLKKTTKAL
                     NMNKKVTTHTFRHTHITLLVEMNVSLKAIMKRVGHVDEKTTIRIYTHVTEKMDRELTQ
                     KLENIPS"
BASE COUNT         1660 a          528 c          744 g         1336 t
ORIGIN      
        1 attaacaaaa cacaaaatag tggatatgaa aatgaatatt tttatatagt tgctaatata
       61 ccgacgctcc aagaatatag gaaatattac gaacccctaa taaagaaaaa taatctgaat
      121 tttaaaaaag gtatgaaaca agcaaggaaa ggagtaggct ataaagctgc aatagaagta
      181 catacaacat tgttttcgag aagtagtaac ttttcaaagg acaaaaaatt agatgatgtt
      241 ttagatttgt ctgaaagtac gaaaaagtta caccttaatt ttgaaaatac gaaaatattt
      301 ttacaactag caaaatctac tattagcact aatcgagtta attacagtga taatgagtct
      361 ataaggattg aggtcgaatg acttgaaatc agctaatttc tctatattct aaacaaacac
      421 ataaatggga gatgggctat gtttaaaaga attaaactaa tcttaatagc aacgataata
      481 ctatcaggat gttcaactac caataacgaa tccaacaatg aaacaaaatc agtaccagaa
      541 gaaatggaag cttcaaaata tgtaggacaa ggcttccaac tacctgcaga aaaagatgcg
      601 attgaatttg cgaagaagca tcgtaaagaa tttgaaaaag taggtgaaca attctttaaa
      661 gataactttg gactaaaagt taaagctaca aatgttgtag gtaaagatga tggtgtagaa
      721 gtttatgtgc attgcgaaga tcatggcatt gtatttaatg caagtctacc tttgtacaaa
      781 gatgccatcc atcaaaaagg atcaatgcgc agtaatgaca atggtgatga tatgagtatg
      841 atggtgggta cagtgctgag tggctttgaa tatcgagcgc aaaaagaaaa gtatgataac
      901 ttatataaat tcttcaaaga aaatgaaaag aaatatcaat atacaggctt tacaaaagag
      961 gcaattaaca agacacaaaa cgttggatat caaaatgaat acttttatat cacatattta
     1021 tcaagaaact taaaagaata tcgtaaatat tacgaaccgt tgattcataa aaatgataaa
     1081 gaatttaaag agggtatgca acgagctaga aaagagttag attatactgc taatagtaat
     1141 acagtagcaa cgttgtttag tacgaatgat aaaaaaaata gaaaagaaaa gataaataat
     1201 gtaatagatt tatccgagaa aattgaaaga acaaaagata tgccaatcaa gaatactata
     1261 actactcaat taggaaataa acttattggc acaaaaaaag ctcgttttga tgataagaaa
     1321 gtagtgtcgt ttggagcatt tgaagatgaa taaaattaat gatagagact taacagaatt
     1381 gagtagttac tgggtttatc aagacatcaa taaagataat gattttacag ttaacggaaa
     1441 aagatttaag caggttgatg aatataatga taatggaaat aaaaataaaa aaggtgcctc
     1501 agatttaaaa atttatgaat tgttggatga aaaaggaaaa ccaactggtg aacaaaccat
     1561 gatttatcaa ggaacatcta atgaggcaat aaaccctaat aatccattaa aatctttaga
     1621 tatcggagat gattggttac aaaatgcgaa attaatggat aatagtaata agtcaacgga
     1681 ttatcttaag caatcagacg aatttgcaga tttatataga gacaaactaa atgacgctaa
     1741 taaattaagt aagtataact ttacacaaaa atatggtgtt agtccaaata attacaaaaa
     1801 caaaaccatt gtggcggatg gcggtaattc ggaaggcggt gcaggagcaa aatatcaagg
     1861 agcgaaacat ccaaatgaaa aagttgttgc tactgaccca gcaatggtac cttatgctgc
     1921 ttggcagaaa tttgctagac cacgctttga taatatgatt agttttaata gtaccaacga
     1981 tttattaaca tggttacaag atccattcat caaagatatg ccaggaaaac gcgttaacat
     2041 tagtgatggt gtgcccaggt tagatgcttt aatagacagc catgtaggtt ataaaaggaa
     2101 gttaaataga aaagacaaca catacgatac tgtaccacta atcaaaatta agtcggtaaa
     2161 agatacagaa attaaaaatg gaaaaaaagt aaaaaagact attaacataa cattagatat
     2221 ggatgggcga attccgataa atgtttggac aggggattcg attgcacgtt ctggaagagg
     2281 aactttaatt aaacttaatt tagaaaatct tgatgcgttg agtaaactga ttactggtga
     2341 aactagtggt atgttagcag aatgcgtaat ctttttaaat gaaagtttta acatctcaga
     2401 aaatgaaaat aaaaattttg cagatagaaa gcaacaatta tcagaaggat ttaaggataa
     2461 gattaactta tttcaattag aagaaatgga aagaacttta attagtaaaa taaactcact
     2521 tgaagaagtt gcagatgaaa caatagaaag tattagtgct gttaaacact tattacctga
     2581 ttttgcattg gatgcattaa aagaaagaat taatgagttg tttaaaggta taaaatcttt
     2641 tatagaaaaa gtgtatgata gtatagataa tgaaatttta gaaattttca aaaatataga
     2701 tcacgacttc agagatggag tatctgaaga aatgatgaaa catttgaaag tagtgaaaca
     2761 gaatatagag cgaataaaaa atcaaaatga tatttatggt aggcaaattg cagaaattag
     2821 aagtattatg aaacaacaag atgcaacaat tttagatggg aatttccaaa ttaattgtag
     2881 cggcgaaaat atggtacagg gtctagttat accttctaat tatttaggaa gaaaaatgaa
     2941 aatattaaaa gaccatatcg atgatggtat taaaaaaata gcagactatg ttcaaagtat
     3001 atatgatgaa tatgcatcga aaattgttga tgtaataaaa tatttgatta atacaattcc
     3061 caaaatacgt aagaatttaa gacatgcaat tgaaatgtta aatgtaaaaa agaaagaatt
     3121 tttgtccctg attcctaatg taacttgtaa ttatattaaa actaaattag aagaattaga
     3181 taatacttta ggcaaatggg agccatttct taatgattta aaagcagtgt caccaatttt
     3241 agataaccat ttagatgata ttgttaagaa catgaagcct ttgattgtac agatgttatt
     3301 tgaaccatca cattatgatg atatgtttat ttcaagaaaa gctttaacgc cagtgttctc
     3361 aagcgtttta taaagcttgt aaaaaatata agggcaaaaa aagggcggat ttaagctaac
     3421 ttggaatgtt ttcgagtttt tgagttagtt ctctatccat tttttcagtt acatgagtat
     3481 atatgcgaat ggttgttttt tcatctacat gtcctaccct tttcataatt gcttttaaag
     3541 aaacattcat ttctactaat aaagttatgt gtgtatgtct aaatgtgtgc gtggtaactt
     3601 tcttattcat atttaaagct tttgtagttt tcttaagcac accggcgatt tgattattac
     3661 ataaaggatt cccttttttt gttgtgaata tgaaccctct gtcaacatag ctcgaattcc
     3721 atcttttcaa cattttgttt tccagtatta tctttttaaa aatttctacg gttctagaat
     3781 tgatgctgat acttcttttt gaacttatag tctttgtagt gtctttgtat ccgaatcctt
     3841 cctcgtattt aatgcggtga attgtacctg ttatattgat agttttgttt aataaatcta
     3901 tatctttttc ctgcagtgct tgtagttctc ctatgcgcat accagttaaa gcctgtactt
     3961 ctaagatgct ggcaattaaa atgcgatttc gcttgtgtaa cttattatca tttagtatat
     4021 gatcacgtat ctgtaggact tggttcattt ctaaatagtt gtacatttta gattcatctt
     4081 tttcgatatc ctctattgtt tttcttcttt taggaatttt gacattagtt aacaaatatt
     4141 catttggata attgtaaaat ttaactgcat atttaatagc tcctttcata tctccgagtt
     4201 gacgggttac ttgattttga gaatagatat ctgataattt attaataaat atctgcatat
     4261 attttgta
//
LOCUS       DANBWH010000207         4229 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.215, whole genome shotgun sequence.
ACCESSION   DANBWH010000207 DANBWH010000000
VERSION     DANBWH010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.215"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(328..558)
                     /locus_tag="PPY92_002349"
     CDS             complement(328..558)
                     /locus_tag="PPY92_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626324.1"
                     /translation="MKQFLYIALVCGVIAGLGAFLHIPQYPSMTIPRIVAILGIISAM
                     LTFKDKQISASLKFSALLINVLPLCGTFVASN"
     gene            complement(589..1527)
                     /locus_tag="PPY92_002350"
     CDS             complement(589..1527)
                     /locus_tag="PPY92_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HDJ2626325.1"
                     /translation="MPLFLQPILKTKLWGGQRLSEFGYQLDNDTTGECWCVSAHPNGT
                     SEIINGPYQGQTLDRIWSEHRELFGDFPSKDFPLLTKIVDARESLSIHVHPDNSYAYE
                     HENGQYGKSECWYIIDAEEDAEIVIGTLAESREEVANHVQHGTIESILRYIKVKPGEF
                     YFIPAGTVHTISSGILAYETMQSSDITYRLYDFNRQDNQYNDRPLNIEKALDVIQYNA
                     PLPNILPESEIIENHKCTHIVSNDFFTLVKWEISGTLNYMKPREFCLVTVLEGEGQMI
                     VDGEIFKLTTGTNFILTSEDLDSVFEGDFTLMISYV"
     gene            complement(1963..2628)
                     /locus_tag="PPY92_002351"
     CDS             complement(1963..2628)
                     /locus_tag="PPY92_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDJ2626326.1"
                     /translation="MNILVIGANGGVGSLLVQQLAKENVPFTAGVRQSDQLNALKSQG
                     MKAILVDVENDSIETLTETFKPFDKVIFSVGSGGNTGADKTIIVDLDGAVKSMIASKE
                     ANIKHYVMVSTYDSRRQAFDDSGDLKPYTIAKHYADDYLRRSGLNYTILHPGALTNAA
                     GSGKIEAAQYFDGKGEIPREDVATVLKEIVTSNHFNHQEFQIISGEQDIKDALTQFEI
                     EKD"
     gene            2780..3100
                     /gene="czrA"
                     /locus_tag="PPY92_002352"
     CDS             2780..3100
                     /gene="czrA"
                     /locus_tag="PPY92_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)-responsive metalloregulatory
                     transcriptional repressor CzrA"
                     /protein_id="HDJ2626327.1"
                     /translation="MSEQYSEINTDTLERVTEIFKALGDYNRIRIMELLSVSEASVGH
                     ISHQLNLSQSNVSHQLKLLKSVHLVKAKRQGQSMIYSLDDIHVATMLKQAIHHANHPK
                     ESGL"
     gene            3102..4079
                     /gene="czrB"
                     /locus_tag="PPY92_002353"
     CDS             3102..4079
                     /gene="czrB"
                     /locus_tag="PPY92_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc efflux transporter CzrB"
                     /protein_id="HDJ2626328.1"
                     /translation="MSHSHHHDHMHSHVTTNNKKVLFISFLIIGLYMFIEIIGGLLAN
                     SLALLSDGIHMFSDTFSLGVALVAFIYAEKNATTTKTFGYKRFEVLAALFNGVTLFVI
                     SILIVFEAIKRFFVPSEVQSKEMLIISIIGLIVNIVVAFFMFKGGDTSHNLNMRGAFL
                     HVIGDLLGSVGAITAAILIWAFGWTIADPIASILVSVIILKSAWGITKSSINILMEGT
                     PSDVDIDEVITTIKKDSRIQSVHDCHVWTISNDMNALSCHVVVDHTLTMKECELLLEN
                     IEHDLLHLNIHHMTIQLETPNHKHDESIICSGTHSHSHNHHAHHHAHVH"
BASE COUNT         1372 a          786 c          595 g         1476 t
ORIGIN      
        1 tttagctacc tattttgcat ataaaaaaca tgcttgcata cactatgcaa taaaaattac
       61 aaatttatat atgataccac tatgcttgct tatctctata gcgccattga tacacatttt
      121 taaatatcta tactgccgtt agaattttat catgtcttaa ttttcattaa atattaatta
      181 cttcatttta tataaaccaa caaaaacccc ctcactacta tgcaagtgag aggttatgtt
      241 gatgtgcttt attttcatca atgtcattaa cacgatcata tttcttctgt taatgcactg
      301 tactttattc aaacgcgcta tcgacaatta atttgaagct acaaaggtac cgcataatgg
      361 cagcacatta attaacaatg cgctaaactt taatgaggcg ctgatttgct tgtctttaaa
      421 agtcaacata gcactgataa ttcctaaaat agctactata cgtggaattg tcatgctcgg
      481 atactgcggt atatgtaaga aagcaccaag acctgctatc acaccacata ctaacgcaat
      541 atatagaaat tgcttcaaat cattcactcc taaattgtta ttacactatt acacatagct
      601 aatcatcaat gtgaaatcgc cttcaaagac actatccaaa tcttcagaag tcaaaataaa
      661 gtttgtacca gtagtcagtt tgaaaatttc accatcgaca atcatttgtc cttcaccttc
      721 caacactgta actaaacaga actctctagg cttcatataa tttaacgtgc cagaaatttc
      781 ccatttaacc aatgtaaaga aatcattcga tacaatgtgt gtacacttat ggttttcaat
      841 aatttcactt tcaggcaaaa tattaggtaa tggtgcattg tactgaataa cgtctaaagc
      901 tttttcaata tttaacggtc tatcattata ttgattatct tgacgattga aatcataaag
      961 tctatatgta atgtctgacg attgcatcgt ttcgtatgct aatattcctg aagaaatagt
     1021 atgtactgtt cctgctggaa taaaatagaa ttctccaggt tttactttaa tatatctaag
     1081 tatcgactct atcgttccgt gttgaacatg attcgcaact tcttctctag actctgctaa
     1141 tgtccctata actatttctg catcttcttc tgcatctata atataccaac attcagattt
     1201 gccatattgc ccgttttcat gctcataagc ataagaatta tcagggtgca catgaataga
     1261 aagtgattct cttgcatcca ctattttagt tagaagcgga aaatctttgc ttgggaaatc
     1321 accaaacaat tcacgatgtt ctgaccaaat acggtctaat gtttgacctt gatatggtcc
     1381 attaataatc tcgctcgtac catttggatg tgctgacaca caccaacatt ccccagttgt
     1441 atcattgtct aattgatatc caaactcact tagacgttga ccgccccata attttgtttt
     1501 taaaattggt tgtaaaaata atggcattgt tgcacctcca ttgtgattaa gtaagcaata
     1561 gaactctgat gttgttgttc cattatattt tgattttgtt ctcatttaca tcgtattata
     1621 aacttccaca tttcaaatta actattagtg attgtaccat atttactaac attgcagtac
     1681 tgccaattaa agcgcttcac ttaaatttac agtactttaa catttttaaa aattaatagc
     1741 atagagatta tctctctctt acatttgtac acatttccct ttaaatttac tcgcccatta
     1801 taccaattaa taaacaactt taatagttgt gccatacatt gttcaaattc tttgtaaaat
     1861 gcatagacaa tacgtactta ttcatactta taattcatca ttttcaaaaa aataacgagt
     1921 tacgaaaacg taacccgctt caaatcatat ttactatcct tattaatcct tttcaatttc
     1981 aaattgagtt aaagcatctt taatgtcctg ctcaccacta ataatttgaa actcttggtg
     2041 attaaaatga ttggatgtga caatttcttt taatactgtc gcaacatctt ctctaggaat
     2101 ttcaccttta ccatcaaaat attgtgcagc ttctatcttt ccagatcctg ctgcatttgt
     2161 aagtgcccct ggatgtaaaa ttgtataatt caaacctgaa cgtcttaaat agtcatcagc
     2221 gtaatgttta gctattgtat atggctttaa atcaccgcta tcatcaaaag cctgacgtct
     2281 cgaatcatat gttgaaacca tgacatagtg tttaatatta gcctctttac tcgcaatcat
     2341 tgatttaaca gcaccatcta aatcgacaat aattgtttta tctgcacccg tgttccctcc
     2401 agaacctact gaaaagataa ctttatcgaa tggtttaaac gtctcagtta aagtctctat
     2461 tgaatcattt tcaacatcaa caagaattgc tttcatacct tgtgatttta acgcattaag
     2521 ttgatctgat tgcctaacac cagcagtaaa tggtacattt tcttttgcta attgttgcac
     2581 tagtaacgaa cctacaccgc cattagcacc tataaccaaa atattcattt acaacactct
     2641 cctatttatt attctctatg ccataccact ttatgagata tgtaaaactt gttacaacta
     2701 taaaaatcaa ttgacatact actgggaacg tattaaatta atatatgaac aaatattcat
     2761 atgaaaggat tgtcatataa tgtcagaaca atattcagaa ataaatacag atacattaga
     2821 acgcgtaact gaaattttca aggcattagg cgattacaat cgaatacgta tcatggaatt
     2881 gttatcagtc agtgaagcaa gtgttggtca catttcacat caattgaatt tatctcaatc
     2941 aaatgtctcg caccaattaa aattacttaa aagtgtgcat cttgtgaaag caaaacgaca
     3001 aggccaatca atgatttatt cattagatga catccacgta gcaactatgt taaagcaagc
     3061 catacatcac gcgaatcatc ctaaagaaag tgggttataa tatgtctcat tcacatcatc
     3121 atgaccatat gcatagtcat gtaactacaa ataataagaa agtattgttt atatcgtttt
     3181 taataatcgg tctatatatg tttatcgaaa tcatcggcgg tctccttgct aacagcttgg
     3241 cattactatc tgacggtatc catatgttta gcgacacatt ctcattaggt gttgcacttg
     3301 tcgcatttat ttatgctgaa aagaatgcca caactacaaa aacatttggt tataaacgtt
     3361 tcgaagtact cgcagcgtta tttaacggtg taacgctttt tgtaataagt attttgattg
     3421 tttttgaagc gattaaacgt ttctttgttc cttctgaagt tcaatcaaaa gaaatgttaa
     3481 tcattagtat tatcggttta attgtcaata tcgttgttgc attctttatg tttaaaggcg
     3541 gcgacacttc acacaattta aatatgcgtg gtgcttttct acatgttatc ggagacttat
     3601 taggttcagt tggcgccatt actgcagcta ttttaatttg ggcatttgga tggacaatcg
     3661 ccgatcctat cgcaagtatt ttagtttccg ttattatttt aaaaagtgct tggggtatca
     3721 caaaatcttc aattaacatt ttaatggaag gcacaccaag tgatgttgat atagatgaag
     3781 ttataactac tattaaaaag gattcacgaa tacaaagtgt gcatgattgc catgtttgga
     3841 caatttcaaa tgatatgaat gcattgagtt gtcatgttgt tgtagaccat acattgacaa
     3901 tgaaagaatg tgaattatta ttagaaaaca ttgagcatga tttattacat ttaaatattc
     3961 accatatgac tattcaatta gaaacgccta atcacaaaca tgatgaatcg attatatgtt
     4021 caggaacaca tagtcattca cataaccatc atgctcatca tcacgcgcat gtacattaat
     4081 aattttaacc tactgccatt gcatcgatta aacttttcaa tggcagtagg ttttttatgt
     4141 ctttatggcg acttgtttgg tctttgatga tgcaatgttt attaacaaat tttcaactat
     4201 tatttcttac attagtcata tttttgaca
//
LOCUS       DANBWH010000208         4121 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.216, whole genome shotgun sequence.
ACCESSION   DANBWH010000208 DANBWH010000000
VERSION     DANBWH010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.216"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            342..1247
                     /locus_tag="PPY92_002354"
     CDS             342..1247
                     /locus_tag="PPY92_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDJ2626329.1"
                     /translation="MTNQDNNHQLNHRIYHFEKIYKAIKHVIVFIFMIFIAIVAIAVI
                     AMSLYFHHLTKTSDSLSDDALIKKVRQIPGDELLDHNNKNLLYEYNHSQNSLIIGPKT
                     SSPNVIKALTSSEDTLFYKHDGILPKAILRAMIQDIFNTDQSSGGSTITQQLVKNQVL
                     TNEKTYSRKANELRLAIRLEHLLSKDEIIYTYLNIVPFGRDYNGANISGIASASYSLF
                     GIPPKDLSIAQSAYLIGLLQSPYGYTPYEKDGTLKSDKDLKYSIQRQHYVLKRMLIED
                     QITEKEYNDALKYDIKSHLLNRKKR"
     gene            complement(1227..1319)
                     /locus_tag="PPY92_002355"
     CDS             complement(1227..1319)
                     /locus_tag="PPY92_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626330.1"
                     /translation="MKIILLLFLIFGCIVVVTLKSEHQLTLFSI"
     gene            complement(1450..>4121)
                     /gene="harA"
                     /locus_tag="PPY92_002356"
     CDS             complement(1450..>4121)
                     /gene="harA"
                     /locus_tag="PPY92_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="haptoglobin-binding heme uptake protein HarA"
                     /protein_id="HDJ2626331.1"
                     /translation="KHHPKLRSFYSIRKSTLGVASVIVSTLFLITSQHQAQAAENTNT
                     SDKISENQNNNATTTQQPKDTNQTQPATQPVITAKNYPAADESLKDAIKDPALENKEH
                     DIGPREQVNFQLLDKNNETQYYHFFSIKDPADVYYTKKKAEVELDINTASTWKKFEVY
                     ENNQKLPVRLVSYSPVPEDHAYIRFPVSDGTQELKIVSSTQIDDGEETNYDYTKLVFA
                     KPIYNDPSLVKSDTNDAVVTNDQSSSDASNQTNTNTSNQNTSTTNNANNQPQATTNMS
                     QPAQPKSSANADQASSQPAHETNSNGNTNDKTNESSNQSDVNQQYPPADESLQDAIKN
                     PAIIDKEHTADNWRPIDFQMKNDKGERQFYHYASTVEPATVIFTKTGPVIELGLKTAS
                     TWKKFEVYEGDKKLPVELVSYDSDKDYAYIRFPVSNGTRDVKIVSSIEYGENIHEDYD
                     YTLMVFAQPITNNPDDYVDEETYNLQKLLAPYHKAKTLERQVYELEKLQEKLPEKYKA
                     EYKKKLDQTRVELADQVKSAVTEFENVTPTNDQLTDLQEAHFVVFESEENSESVMDGF
                     VEHPFYTATLNGQKYVVMKTKDDSYWKDLIVEGKRVTTVSKDPKNNSRTLIFPYIPDK
                     AVYNAIVKVVVANIGYEGQYHVRIINQDINTKDDDTSQNNTSEPLNVQTGQEGKVADT
                     DVAENSSTATNPKDASDKADVIEPESDVVKDADNNIDKDVQHDVDHLSDMSDNNHFDK
                     YDLKEMDTQIAKDTDRNVDNSVGMSSNVDTDKDSNKNKDKVIQLAHIADKNNHTGKAA
                     KLDVVKQNYNNTDKVTDKKTTEHLPSDIHKTVDKTVKTKEKAGTPSKENKLSQSKMLP
                     KTGETTSSQSWWGLYALLGMLALFIPKFRKESK"
BASE COUNT         1241 a          692 c          630 g         1558 t
ORIGIN      
        1 ttaaaatact ttaaagtttt ttgaacgtaa aaaaaaccct tacaacaaat atgtaagggc
       61 gcgattgcac gttaccacca aacttaaaca taatcataag ataatgttca ctctattaat
      121 gatacgttca ttaataaacg taggacatgt tagttataaa ggtgtattca tattattaaa
      181 aacactagtt cacagcgacc actagctctc tgatgatttc aaataatatt acttgtcctt
      241 ttatcctatt ctttatgcat tttccctaat tataattaac gttaaaataa aagtcaaatt
      301 gcttaaatat ggtatactat acgtaattta ggaggttaaa gatgacgaat caagacaaca
      361 atcatcaatt gaatcatcgt atatatcatt ttgaaaagat atataaagct atcaaacatg
      421 tcattgtttt tatatttatg attttcattg ccatcgttgc tatcgctgtg attgcgatgt
      481 ctttatattt tcatcattta actaaaacgt ccgactcatt atcagatgat gctttaataa
      541 aaaaagttcg acaaatacct ggcgatgaat tattagatca taataacaaa aatttattat
      601 atgagtataa ccattctcaa aactcactca ttataggccc taaaacatca agtccaaatg
      661 tcattaaagc attaacatca tctgaagaca ctttatttta taaacatgat ggcatcttac
      721 caaaggcgat tttaagagca atgatacaag atatttttaa tactgatcaa agttcaggtg
      781 gtagcacaat tacacaacaa cttgttaaaa atcaagttct taccaacgaa aaaacatata
      841 gtagaaaagc aaatgaactt cgcctagcaa ttagattaga acacctactc tcaaaagatg
      901 aaattatata tacatattta aatatagttc ccttcggtag agattataat ggcgctaata
      961 tttccggaat tgcatccgct tcatatagtt tgtttggtat tccaccaaaa gatttatcaa
     1021 ttgcacaatc tgcatacctt atcggtttgt tgcaaagccc ttatggctat acaccctacg
     1081 aaaaagatgg aacgttaaaa tcggataaag atttgaaata tagtattcaa agacaacatt
     1141 atgtattaaa gcgtatgtta atcgaagatc aaatcactga aaaagaatac aacgacgcat
     1201 taaaatatga tattaaatca catttgttaa atcgaaaaaa gcgttaattg atgctcactt
     1261 tttaaagtaa ccacaacaat gcatccaaat attaaaaaca gcagtaagat tattttcaat
     1321 tagaaaattt ctcactgctg ttctttttat ttactctaaa ggaaagccat taaaccaatc
     1381 aatagtttat aaattaattg ctttcacatt gtttacaaag ttaaccatac atatgctata
     1441 tttagttaat tatttagatt cttttctgaa tttaggaatg aataaagcta acatacctaa
     1501 taacgcatat aagccccacc atgattggct tgaagttgtt tctccagttt ttggtagcat
     1561 tttagattga ctaagtttgt tttctttcga tggtgtgccg gctttttctt ttgttttcac
     1621 tgttttatct acagttttat gaatatcact cggcagatgt tcagttgttt ttttgtcagt
     1681 aactttgtct gtattattat aattttgttt cactacgtca agctttgctg cttttccagt
     1741 atgattattt ttatcggcaa tatgagcaag ctgtatgact ttgtctttat ttttattaga
     1801 gtctttatca gtatcgacat tcgatgacat accaacgcta ttatccacat ttctatcagt
     1861 atctttggca atttgagtat ccatttcttt taaatcatac ttatcgaagt gattattatc
     1921 cgacatatcg gataaatgat caacatcatg ttgcacatct ttatcaatat tattatcagc
     1981 atctttaacc acgtcagact ctggttctat cacatctgct ttatcagacg catctttagg
     2041 atttgttgca gtgctgctat tttcagctac atctgtatca gcaaccttac cttcttgtcc
     2101 tgtttgtaca tttagcggtt cactcgtgtt attttgtgat gtatcatcat cttttgtatt
     2161 gatatcctga tttataattc tgacatgata ttgaccttca taaccaatgt ttgccacaac
     2221 gactttaaca atcgcattgt aaactgcttt gtcaggtata tatgggaaaa tcaacgttct
     2281 agaattattt ttaggatctt tagaaacagt agtgacacgt ttaccttcta caattaaatc
     2341 tttccagtaa ctgtcatcct ttgttttcat cactacatat ttttgaccat ttaaagttgc
     2401 tgtatagaat ggatgttcaa caaagccgtc cataactgac tcactatttt cttcactttc
     2461 aaaaacaaca aaatgcgctt cttgtaaatc tgttaattga tcatttgtag gtgtaacatt
     2521 ttcaaattcc gtcactgctg atttaacctg atcagctaac tctactctag tttgatctaa
     2581 tttcttttta tattccgcct tatatttttc tggcaatttc tcttgtaatt tttctaattc
     2641 ataaacttgt ctttctaacg ttttagcttt gtgatacgga gctaataatt tttgtaaatt
     2701 gtatgtttcc tcatccacat agtcgtctgg gttattagta ataggctgtg caaagaccat
     2761 tagcgtataa tcatagtctt catggatgtt ctcaccatat tcaatagatg acacaatttt
     2821 aacgtctctc gtaccattag atactgggaa acgaatatag gcataatctt tatcagaatc
     2881 atatgatact aattcgactg gtaacttttt gtcaccttca taaacttcaa atttcttcca
     2941 tgttgaagct gtctttaaac ctaactcaat tactggacct gtttttgtaa aaatgacagt
     3001 tgctggttca acagtactag cataatgata gaactgtctt tcacctttat catttttcat
     3061 ttgaaaatca attggtcgcc aattatcagc tgtatgttct ttatcgatga tagccgggtt
     3121 tttaattgca tcttgtagtg attcatctgc tggtggatac tgttgattaa catccgactg
     3181 attacttgac tcattcgttt tatcgttagt attaccatta gaatttgttt catgagctgg
     3241 ttggcttgac gcttgatctg cattcgctga cgattttggt tgtgcaggtt gactcatatt
     3301 tgtcgttgcc tgcggttgat tattagcatt gttggtcgtt gatgtatttt gattagatgt
     3361 attcgtgttt gtttgattac ttgcgtctga acttgattga tcattcgtta ctactgcatc
     3421 atttgtatct gatttcacaa gtgaaggatc gttataaata ggtttagcaa atactaattt
     3481 agtataatca taatttgttt cttctccatc atcaatttga gtcgaagaaa caattttcaa
     3541 ttcttgtgtg ccatctgaaa ctgggaatcg aatataggca tggtcttctg gtacaggact
     3601 atatgataca agtctcactg gtaatttttg attgttttca tagacttcaa actttttcca
     3661 tgttgaagca gtattgatgt ctaattcaac ttctgctttc tttttagtgt aatacacatc
     3721 tgctggatct ttgatgctga aaaagtgata gtactgcgtt tcattgtttt tatctaataa
     3781 ctggaaattg acttgttctc ttggacctat atcatgttct ttattttcta atgcaggatc
     3841 tttaattgca tctttaagtg attcatccgc tgcaggatag tttttcgcag tgattactgg
     3901 ttgcgtagca ggttgtgttt gatttgtatc cttaggttgc tgagttgtag ttgcattatt
     3961 attttgattt tccgagattt tatctgaagt atttgtattt tctgctgctt gcgcttgatg
     4021 ttgagaagta attaaaaata gtgtactgac aatgaccgat gcaacgccta gagttgattt
     4081 tctaatagaa tagaaagacc ttaattttgg gtgatgtttg t
//
LOCUS       DANBWH010000209         4106 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.217, whole genome shotgun sequence.
ACCESSION   DANBWH010000209 DANBWH010000000
VERSION     DANBWH010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.217"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            198..1538
                     /gene="brnQ"
                     /locus_tag="PPY92_002357"
     CDS             198..1538
                     /gene="brnQ"
                     /locus_tag="PPY92_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDJ2626332.1"
                     /translation="MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFV
                     LTGIGLPLLGVIVGALDKEGYIGALNKISPKFSILFLIIIYLTIGPLFAIPRTASTSF
                     EMTITPIIHSNSSIALFIFTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMII
                     KGYLDFSGNSAGKGNEALYHSNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGI
                     TKTNQIFKQTLTAGLIAVALIFIYISLGYIGNHMPVSDMTLDQLKSKDRNIGTYLLTT
                     MASTGFGSFGKYLLGIIVALACLTTACGLIVAVSEYFHRIVPKVSYKAFVLVFILMSF
                     IIANQGLNAVISMSIPVLSIVYPVAITVVLLILIAKFIPTKRISQQIPVIIVFILSIF
                     SVISKLGWLKINFIESLPLRAYSLEWFPVAIIATILGYLVGIFVKQDPIKYQQE"
     gene            complement(1611..2747)
                     /locus_tag="PPY92_002358"
     CDS             complement(1611..2747)
                     /locus_tag="PPY92_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDJ2626333.1"
                     /translation="MTENKSFKESHPLDDFISDKELSNTTIQKEKLTIEQQKQVDTIS
                     KQINPLDNEGLLAFGSDLQKQMSQFSHQMLDEVQSKDVGPIGDTLSDLMSKLKSVNPN
                     ELNTDKPSMLKRIFSRAKSSINEIFSRMQSVSAQVDRITIQLQKHQTHLTRDIELLDT
                     LYDKNKQYFDDLSLHIIAAQQKKLQLENEKLPQLQQQAQQSTNQMDIQQVSDMQQFID
                     RLDKRIYDLQLSRQIALQTAPQIRMIQNVNQALAEKIQSSILTSIPLWKNQMAIALTL
                     MRQRNAVAAQRAVTDTTNDLLTANAEMLKQNAIETATENERGIVDLDTLKRTQRNIIE
                     TIEETLIIQQHGREERQLAEKELQQLEQDLKSHLVNIKGPNKQS"
     gene            complement(2779..3408)
                     /locus_tag="PPY92_002359"
     CDS             complement(2779..3408)
                     /locus_tag="PPY92_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDJ2626334.1"
                     /translation="MTVRYNISHIFGVLVGIPVAFLTSIFGMIALDVSFLIDMSIGIV
                     GFLMTYLPIQKLTSRKYLNEIGLTRKDYRYIRNQLNHTHQKLRGILKTYVNIRSIKDF
                     RQINDIYQISRSIYTTVRQRPASFYKVEGFFYSHIDNALNLVDAYTRLAKMPKKSINE
                     QQKLEQTRITLDEVKRTLIADLKRLNEDDYERLDIEMELNKLHQKHHQD"
     gene            complement(3427..3696)
                     /locus_tag="PPY92_002360"
     CDS             complement(3427..3696)
                     /locus_tag="PPY92_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDJ2626335.1"
                     /translation="MRHIHLQVFGRVQGVGFRYFTQRIAMNYNIVGTVQNVDDYVEIY
                     AQGDDADIERFIQGVIEGASPASNVTSHQLEELELNQKLSDFRSI"
     gene            3859..>4106
                     /locus_tag="PPY92_002361"
     CDS             3859..>4106
                     /locus_tag="PPY92_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HDJ2626336.1"
                     /translation="MWTVTKIRADYEGWWLFSDWPENIVEKYQYQDFDDMFKHYQQLI
                     NQCKVQFDNYVTGKYNIYAFYNNCDMNYCEDCEEDLQI"
BASE COUNT         1249 a          634 c          651 g         1572 t
ORIGIN      
        1 attgtgaata aaagtcatca gttactattt aaaaagtgaa tttgaagaat tttattttct
       61 ggcataattt gatttaaaca acacatgtgt tttgtttctg ctatgtacaa catatttcat
      121 atagactctt tattattttt tgtgctaaaa tatttacttg taaattattt cgtgttttaa
      181 aaaaaaggag taactctatg aataaaaata catgggtcat tgggtttacg ctcttcgcta
      241 tgttttttgg cgcaggcaat ctaatttttc ctcctaatct cggattggat agtggtcaat
      301 ttttctggcc cgccatttta gcgtttgttc taactgggat tggtttacca ttattaggtg
      361 tgattgtagg tgcacttgat aaagaaggat atattggcgc attaaataaa atttcaccta
      421 aattttcaat attgttctta atcatcattt atttgactat aggaccactt ttcgcaatac
      481 ctagaactgc atctacatct tttgaaatga caattacacc aattatacat agcaatagta
      541 gtatcgcttt atttatattt acgattatct acttcatagt cgttttgtat atttgtttaa
      601 atccatctaa gttaatcgat cgtattggtt cattattaac accattatta ttgattacta
      661 ttttagcgat gattattaaa ggatacttag actttagcgg taatagtgct ggaaagggca
      721 atgaagcact atatcattct aatttttcaa gttttgctga aggctttaca caaggctatt
      781 taacaatgga tgccattgca gcaattgctt tttcaatgat tgttgttaat gcagtaaaac
      841 taacaggcat tactaaaaca aatcaaatat tcaaacaaac tttgactgct ggtttaattg
      901 ccgtagcttt aattttcata tatatttcat taggttatat tggtaatcat atgccagtaa
      961 gtgacatgac gttagatcaa ttgaaatcca aagatcgaaa cattgggaca tatttattaa
     1021 cgacaatggc ttcaacagga tttggttcat tcggaaaata tttattgggc atcattgtgg
     1081 cgctggcatg tctaactaca gcatgcgggc ttattgttgc agtttctgaa tatttccata
     1141 gaatcgtacc taaagtatca tacaaagcat ttgtattagt tttcatttta atgagtttta
     1201 ttattgctaa ccaaggttta aatgctgtta tctcaatgtc aattccggta ttaagcattg
     1261 tatacccagt agcaataact gttgtattat taattttaat tgccaaattc ataccgacaa
     1321 aacgcatttc acaacaaatt ccagttatta tcgtatttat attgtcgatt ttcagtgtta
     1381 ttagtaagtt aggttggctg aaaattaact ttatagaatc attgcctcta agagcgtatt
     1441 ctttagagtg gttcccagta gcaattattg caacgatatt aggctatcta gtcggcatat
     1501 ttgtaaaaca agatccaatt aaatatcaac aggaataacg aataatataa aaggaagcat
     1561 ctccaattta atcgtaggat gcttcctttt tgttatatct aattcgtaat ttatgattgt
     1621 ttattcggtc ctttgatgtt cactaaatgt gactttaaat cttgttctaa ttgttgtaat
     1681 tctttttcag ctaattgtcg ttcttcgcga ccgtgttgtt gaataattaa tgtttcttca
     1741 attgtctcaa taatgttacg ctgtgtacgt ttcaatgtat caagatcaac aatgccacgc
     1801 tcattttctg ttgcagtttc aatcgcattt tgtttcaaca tttcagcatt tgctgttaat
     1861 aaatcattag ttgtatcagt gacagctcgt tgtgcagcaa ctgcattacg ctgtctcatt
     1921 aatgtaagcg caatggccat ttgatttttc catagtggaa tacttgtcaa aattgaactt
     1981 tgtatcttct cggcaagtgc ttgattaaca ttttgaatca tacgaatttg tggcgcagtt
     2041 tgcaaagcta tttgtcttga aagctgtaag tcatatatgc gtttatctag tctatctata
     2101 aattgctgca tatctgaaac ttgttgaata tccatttgat tagtggattg ctgcgcttgc
     2161 tgttgcaatt gtggtagctt ttcattttct aattgcaact ttttttgctg tgcagcaatg
     2221 atatgcaatg ataagtcatc aaagtattgt ttgtttttat catatagcgt atctaataat
     2281 tcaatatctc ttgttaaatg tgtttgatgt ttctgcagtt gaatcgttat gcgatcgact
     2341 tgagcactaa ctgattgcat tcttgaaaag atttcattga tagacgactt tgctctgcta
     2401 aaaattcttt ttaacataga tggtttatca gtatttaact catttggatt aactgacttt
     2461 agttttgaca ttagatctga caaagtatct ccaataggac caacatcttt actttgtact
     2521 tcatccaaca tttgatgtga aaattgagac atttgtttct gtaaatcaga accaaacgct
     2581 aataaacctt cattgtctaa agggttaatt tgtttactga ttgtgtctac ctgtttttgt
     2641 tgttcaattg ttaacttttc tttttgaata gtagtattcg ataattcttt atcgcttata
     2701 aaatcatcta gtggatggct ttctttgaaa cttttatttt cagtcatcaa tcatctctcc
     2761 aatagttgct attttacatc aatcttgatg atgtttttga tgtaacttat ttaattccat
     2821 ttcaatatct aaacgttcat aatcatcttc gttgagacgc tttaaatcag cgattaatgt
     2881 tcgtttgacc tcatccaaag taattcgtgt ttgttctaac ttttgctgtt cattaattga
     2941 ttttttgggc atttttgcta gacgtgtata tgcatcaacc aaatttaaag cattatcaat
     3001 atgagaataa aaaaagcctt caactttata aaatgatgca ggtctctgtc taactgtcgt
     3061 ataaatagaa cgtgaaattt ggtatatatc attaatctgc ctaaaatctt taattgatct
     3121 tatattgaca tacgttttta aaatacctct aagtttttgg tgtgtatgat ttaactgatt
     3181 tcgaatatag cgatagtctt ttctagtcaa accaatttcg tttaaatatt tgcgtgaagt
     3241 gagtttttgt atcggtaggt atgtcattaa aaagccaaca ataccaatag acatatcaat
     3301 taaaaaagat acatcaagtg caatcatccc aaatatgctt gttaaaaacg ctacaggaat
     3361 tcccactaac accccaaata tatgagaaat attatatctc actgtcatct tcctttattt
     3421 agcattttat attgatcgaa aatccgataa tttttgattt agttctaact cttcaagttg
     3481 atggcttgtt acatttgatg ctggtgaggc accttcaatt acaccttgaa taaatctctc
     3541 tatatctgcg tcatcccctt gtgcatatat ctctacatag tcatctacat tttgaacagt
     3601 accgacaatg ttatagttca ttgcaatgcg ttgtgtaaaa tatctaaatc cgacgccttg
     3661 aacgcgtccg aatacttgta aatgtatatg tctcattttt accacctcat aatgttatta
     3721 tacgtagttt tacttaaaaa aactaataat tactatagtt actactttgt ttgtttcaag
     3781 tcgtcaaact tgattttcag aggataaagg tataaaaata agtatagagt ttttgaagta
     3841 tggaaggggt ctttaataat gtggacagtt accaaaatta gagccgatta tgagggatgg
     3901 tggttattca gtgactggcc agaaaacatt gttgaaaaat atcaatatca agattttgat
     3961 gacatgttta agcactatca acaattgatt aatcaatgta aagttcagtt cgataattat
     4021 gtcacaggca aatataatat ttatgcattt tataataatt gtgacatgaa ctattgcgaa
     4081 gattgtgaag aggatttaca aatatt
//
LOCUS       DANBWH010000210         3913 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.218, whole genome shotgun sequence.
ACCESSION   DANBWH010000210 DANBWH010000000
VERSION     DANBWH010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.218"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            30..404
                     /locus_tag="PPY92_002362"
     CDS             30..404
                     /locus_tag="PPY92_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002441964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDJ2626337.1"
                     /translation="MELNYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDL
                     MFNNKNGDTLIKDGVPKDYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKD
                     NKSDKPNDKLISETAKSVMKEF"
     gene            complement(930..1349)
                     /locus_tag="PPY92_002363"
     CDS             complement(930..1349)
                     /locus_tag="PPY92_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDJ2626338.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLIN
                     SNLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWP
                     TLEKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
     gene            1473..2444
                     /locus_tag="PPY92_002364"
     CDS             1473..2444
                     /locus_tag="PPY92_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDJ2626339.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKD
                     MLALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEY
                     AQVPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGG
                     VGSVAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLD
                     TVGSNVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIR
                     EDIWSKLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
     gene            complement(2616..2708)
                     /locus_tag="PPY92_002365"
     CDS             complement(2616..2708)
                     /locus_tag="PPY92_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDJ2626340.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
     gene            complement(2865..3212)
                     /locus_tag="PPY92_002366"
     CDS             complement(2865..3212)
                     /locus_tag="PPY92_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDJ2626341.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKK
                     LKIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDD
                     EIREFFSKNHEGF"
     gene            complement(3231..3848)
                     /gene="cadD"
                     /locus_tag="PPY92_002367"
     CDS             complement(3231..3848)
                     /gene="cadD"
                     /locus_tag="PPY92_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium resistance transporter CadD"
                     /protein_id="HDJ2626342.1"
                     /translation="MIQTVVAAAVLYIATAVDLLVILLIFFARAKTRKEYRDIYVGQY
                     LGSIILILVSLFLAFVLNYVPEKWILGLLGLIPIYLGIKVAIYDDCEGEKRAKKELNE
                     KGLSKLVGTVAIVTIASCGADNIGLFVPYFVTLSVTNLLLTLFVFLILIFFLVFTAQK
                     LANIPGIGEIVEKFSRWIMAIIYIALGLFIIIENDTIQTILGFIF"
BASE COUNT         1463 a          563 c          543 g         1344 t
ORIGIN      
        1 ataaagtaac aaatccagtt agatatgaga tagaattaaa ttactattca ccaaagagca
       61 aaaaagatac ttcaacgcct gctgctttcg gcaagacttt aaataaactt atcgcaaatg
      121 gaaaattaag caaaaaaaat aaaaatttct tacttgattt aatgtttaat aataaaaacg
      181 gagacacttt aattaaagat ggtgttccaa aagactataa ggttgctgat aaaagtggtc
      241 aagcaataac atatgcttct agaaatgatg ttgcttttgt ttatcctaag ggccaatctg
      301 aacctattgt tttagtcatt tttacgaata aagacaataa aagtgataag ccaaatgata
      361 agttgataag tgaaaccgcc aagagtgtaa tgaaggaatt ttaatattct aaatgcataa
      421 taaatactga taacatctta tagtttgtat tatattttgt attatcgttg acatgtataa
      481 ttttgatatc aaaaactgat tttcccttta ttattttcga gatttatttt cttaattctc
      541 tttaacaaac tagaaatatt gtatatacaa aaaatcataa ataatagatg aatagtttaa
      601 ttataggtgt tcatcaatcg aaaaagcaac gtatcttatt taaagtgcgt tgcttttttc
      661 tcatttataa ggttaaataa ttctcatata tcaagcaaag tgacaatagc attttacaag
      721 tattttttat tgctcgatta gggtaaacct aaataataca taaattatat tacaatatgt
      781 ttattataga ggctaaagta tcttagaaat ttggatagta ggtttttcgg gaataaaagt
      841 atagttggct ttaatatata attaagctaa gctatcattt atatttactc gattataatt
      901 cgcacttcaa ctttttaata acgtaaaaat taaaacgtag ttttatttat ctttagtaat
      961 atctttttaa acactacagt ttcttcatta gttaaattag cgaagtaatg attattaagt
     1021 tgataggctt tcttttctaa tgttggccat aagtcttttg ctttatttgt actgtttatt
     1081 agcacagttc ttctattttc attatcagtt tgacgtgtta ttagatcctt tttggacatc
     1141 ttatctaata ttccagatat tgttgaaggt tctaaattta aagtattacc aatcgcttta
     1201 ggtgtattca aattactatt aatcaagcaa ttcaaaacac catattctgc tggagtaata
     1261 ttatagggac ttaataaact tttaaaatgt ttaaaaactc tattctgaga agtactcaac
     1321 aaataattta tacaattatc taattccaca actaaaacct ctcttaaatc attagtattg
     1381 actcgcttat catatcatga taagttttgt aggaaaatag ttttgcaaca aaaacatact
     1441 tctacaaaac aattgtgaaa gaaggctagt aaatgttcaa tgcggtagta gtgagaaaaa
     1501 gtgcatcaaa tgttagttat aaagtagaaa aagttgatga atctcattta tcgaagggtg
     1561 atgtattagt aaaagtggta tactcttcaa taaattataa ggatatgcta gctttacaat
     1621 ataaaggcgg tgtaattaga gattatccta tgattcctgg tatagatttt gcaggtattg
     1681 ttgaatcaag ttcgaatgat aagtttaaag aaggcgataa tgtattagtt actggttatg
     1741 atgttggcgt gacacataca ggtggatttt cagaatatgc acaagtgcct ggagagtgga
     1801 ttgttcctat tccccaagaa atgtctatta aagagtctat ggtatatggc acagccggtt
     1861 ttacggcagc attatcaatt tttgaactag aaaaatatgg aatgaatatc aaatcacaac
     1921 ctgaaatttt agtgtctggg gctacaggtg gtgtgggcag tgttgctata caaattttac
     1981 ataaaattgg atatgaaaat atcactgcta tcgtaagaaa agaaaagcaa atagaaatcg
     2041 caaaaaaatt aggtgcaaaa caagtaataa taattgaaga tgataataaa ccgcttaaaa
     2101 aaggtatttt tgattatttc ttagatacag tggggagtaa tgtaacatta tacggtttaa
     2161 aacgtctaaa ttatcaaggt gttgctactg tatgtggaaa tgtagcagga aattcactta
     2221 acgctaatat attaccgttt attcttagag gtattaaatt aattggcatt gattctgtat
     2281 atgttgatca taatatccgt gaagatatat ggagtaagtt agcaaatgaa tggaatatag
     2341 gaaattcact tctatacaat gaaattggat ttgaagaatt ctataaaacg atagatcagc
     2401 ttttaaaagg acaacattta ggaagaacta ttttaaaagt ataagtgtga gtattcatta
     2461 atacatttac tattaattca atcattttgc atttctctac taaccagtgt tataatttat
     2521 ataacgaaaa tgattaacaa ttaatgcacc aatccccaca ctattgcagt agtgtgggga
     2581 ttttttcgat gctggcttat aaagtcgcct attaattatt tgttgcgttt actaagccaa
     2641 taagtataat acgcaacagc acagccactg atgactggtg ctatgatgtg aacgaaaatg
     2701 attagcaaag taatgcacct cctctcttcg tcaattgacg cctgagagat aggcgactct
     2761 attattatac catctgtgaa tatacaagca tatctacttc cgataagtcg tcttatgtta
     2821 tataactttg ttttttatag tatcaagaca agaagaaact cgtttcaaaa accctcatga
     2881 tttttagaga aaaactctct tatttcatcg tctttaataa aataatatgc catctttcca
     2941 tctttataaa aatcaagtac ctcattttta tataaaagcc ttaaatgatg tgaagttgaa
     3001 gcaacactca ttttcaatat caaagaaata tcacaaacac acaactcatc ttctttaatc
     3061 aaagataata tgattttcaa tttcttctca tcacaaattt tttctaaaat gttaagtaat
     3121 ttcttagatt tatcatcttc taaaaaactt aaagcattgt taactttatc ctcatgtaca
     3181 cagatcacat cacaagtatt ttcataactc atataaaatc acaccctaaa ttaaaatata
     3241 aatcctaaaa ttgtttgaat agtgtcattt tcaataataa taaataaacc taaagctata
     3301 taaataatag ccataatcca acgactaaat ttctcaacaa tttctccaat tcctggaata
     3361 ttagctaatt tttgtgcagt aaacaccaag aagaaaatta aaattaaaaa gacaaacaga
     3421 gtaagtaata aattagtaac acttaatgtc acaaaatacg gaacaaataa accaatatta
     3481 tcggcgccac aacttgctat cgtaacaatt gcaaccgtac caactaattt agacaatcct
     3541 ttttcattca attctttttt agctctcttt tctccttcac aatcatcata aatagccact
     3601 ttaattccaa gataaattgg tattaaaccc aataaaccta atatccactt ttctggaaca
     3661 taatttaata caaaagctaa aaacaaacta actaatatta aaataataga ccctaaatat
     3721 tgaccaacat aaatatctct atattctttt ctagtctttg ctctagcaaa aaatattaat
     3781 aaaattacta ataaatcaac tgctgtagca atataaagaa cagcagcagc aacaaccgtt
     3841 tgaatcataa tacacctcat tcaaaaatat ttttgaatgt attataactt atatttttaa
     3901 ataatcatag gtg
//
LOCUS       DANBWH010000211         3907 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.219, whole genome shotgun sequence.
ACCESSION   DANBWH010000211 DANBWH010000000
VERSION     DANBWH010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.219"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..1867)
                     /locus_tag="PPY92_002368"
     CDS             complement(<1..1867)
                     /locus_tag="PPY92_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation protein NorD"
                     /protein_id="HDJ2626343.1"
                     /translation="MSDRFIKFNDEQLDAKQVMMLQDLARLLLKNEQTQVKIQKFPYY
                     NPVQNVLITSWFWSHRPSHIEMAGLKTDVMLAAYGYHMMDVQIVNEVVQDKTFKHPKF
                     YQQLFKLLEDMRVLNSIKVERPSTAKLIDLRLDTRVSYTESQIKVYRTKTQYTDLLFL
                     YLEHAFLSQDFFDIPSIHSDLDDILVNMFLYLPNFFQNQNSEDNMYLAQRIMYQVDDI
                     LKEDMLNEYYYLPKTLYNTLASPEFDDLKRTDASQVDGQDDTSEEDDNESEKADSKSA
                     DSESKGGAYLEMELHEGQNSETLGNDEAREGDATDDMTDMMTKKGKGSNDTLNREEGD
                     AVGQSQAFQLDGVNKNVEIKWQIPEIEPQYVLEYQESKQDVQYEIKDLIQIIKKTIER
                     EQRDARFNLTKGRLQKDLINWFIDDQYKLFYKKQDLSKSFDATFTLLIDASASMHDKM
                     AETKKGVVLFHETLKALNIKHEILSFSEDAFDSDDHAQPNIINEIINYDYSTFEKDGP
                     RIMALEPQDDNRDGVAIRVASERLMRRNQHQRFLIVFSDGEPSAFNYSQDGIIDTYEA
                     VEMSRKFGIEVFNVFLSQDPITEDVEQTIHNIYGQYAIFVEGVAHLPGHLSPLLKK"
     gene            complement(1881..2672)
                     /locus_tag="PPY92_002369"
     CDS             complement(1881..2672)
                     /locus_tag="PPY92_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDJ2626344.1"
                     /translation="MALKHYKNSDSTVFNDAKALFDLNKNILLKGPTGSGKTKLAETL
                     SEVVDTPMHQVNCSVDLDTESLLGFKTIKTNAEGQQEIVFVDGPVIKAMKEGHILYID
                     EINMAKPETLPVLNGVLDYRRQITNPYTGEVIKAVPGFNVIAAINEGYVGTLPMNEAL
                     KNRFVVIHVDYIDGDILKNVIKEQSLLQDDKQIEQIIKFNEDLRTMSKQGQISEEAAS
                     IRALLDLCDLITVMPVERAIKRTIIDKLEDEREQQAIYNAVELNF"
     gene            complement(2853..3056)
                     /locus_tag="PPY92_002370"
     CDS             complement(2853..3056)
                     /locus_tag="PPY92_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626345.1"
                     /translation="MLHETWKERTPIKKVEVINTDAKKFTVSDMLTVGKQYDVINETE
                     EYYQIIDNSGLVGGYYKTYFKEV"
     gene            complement(3085..3894)
                     /locus_tag="PPY92_002371"
     CDS             complement(3085..3894)
                     /locus_tag="PPY92_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDJ2626346.1"
                     /translation="MCGLRSITLGTTNIEQTKHFMVDILGLNYEELLENSIRFGDADI
                     SPGTRLQFIQVPSEQLEESHFVGIGLRTPTDSGLEEYAEILSNKDIPFTTVKELNGNK
                     YFSLEDNNGHIFSIYSNENNYGVGLGMPSFESAVNPLHQVQGLGPVILKVNHVDITGQ
                     ILTNIFGLEVFAEYQPFDNADYHVQVFKVGTGGLGGEIHLMPVETEMTMPEYGAVDQV
                     EFETKDADFFNQAKSRLDEVEIPYQTLEQDDIESIRITENSGLSFIFTLQK"
BASE COUNT         1191 a          715 c          537 g         1464 t
ORIGIN      
        1 atttttttaa taatggagag agatgtccag gtaaatgcgc aacaccttca acgaaaatag
       61 catattgacc ataaatatta tgaattgttt gttcaacatc ttcagttatt ggatcttgac
      121 tcaaaaatac attaaatact tcaataccaa atttacgtga catttctaca gcttcatacg
      181 tatcaataat accatcttga ctataattaa atgcagacgg ttcgccgtct gaaaatacga
      241 ttaaaaatcg ttgatgttga tttcgacgca ttaatcgttc acttgcaact ctaatagcaa
      301 caccatcacg attatcatct tgaggttcaa gtgccataat acgtgggcca tctttttcaa
      361 aggttgagta atcatagtta ataatttcat taatgatatt tggttgtgca tgatcgtctg
      421 aatcaaaggc atcctcactg aatgataaaa tttcatgttt aatgttcaat gcttttaacg
      481 tctcgtggaa taacactaca cctttcttcg tttcagccat tttatcatgc atacttgctg
      541 acgcatcaat taacaatgta aacgtcgcat caaatgattt acttaaatct tgttttttgt
      601 aaaacaattt atattgatcg tcgataaacc aattaattaa atccttttgt aatcgccctt
      661 ttgttaaatt aaaacgtgca tctcgttgtt ctcgttcaat cgttttcttt ataatttgaa
      721 ttaagtcttt gatttcatat tgtacgtctt gtttcgattc ttgatattct aaaacatatt
      781 gtggctcaat ttcaggaatt tgccatttta tttctacatt tttgttcaca ccatcaagtt
      841 gaaatgcttg gctttgacca acagcatcgc cttcttcacg atttagcgta tcgtttgatc
      901 ctttgccctt tttggtcatc atatcagtca tatcatctgt agcatcgcct tcacgtgctt
      961 catcattacc taatgtttca ctattttgtc cttcgtgaag ctccatttct aaataggcgc
     1021 caccttttga ctcactatct gcagatttag agtcagcttt ttcactttcg ttatcatcct
     1081 cttctgaagt atcatcttgc ccatcgactt gacttgcatc agttctcttt aaatcatcaa
     1141 actctggact agctaatgta ttatatagtg ttttaggtaa gtaataatat tcgtttagca
     1201 tatcttcttt taaaatatca tctacttgat acataattct ttgagccaga tacatattat
     1261 cttcagaatt ttgattttgg aaaaaattag gtaaatataa gaacatattt actaaaatat
     1321 catctaaatc tgaatgtata gatggtatat caaagaaatc ttgacttaaa aatgcatgtt
     1381 ctaaatacaa aaataataaa tctgtatatt gtgttttagt acgatacact ttaatttgag
     1441 attccgtata tgatacccgt gtatctaagc gaagatcaat taatttagca gtacttgggc
     1501 gctcaacttt aatagaattt aatacgcgca tatcttctaa taatttaaaa agttgttgat
     1561 aaaatttagg gtgtttaaaa gttttatctt gtactacttc atttacaatt tgtacatcca
     1621 tcatatgata accgtaagca gctaacataa catctgtttt taaaccagcc atttcgatat
     1681 ggcttggtcg atgtgaccaa aaccaactcg taatcaaaac attttgaaca ggattatagt
     1741 aagggaattt ttggatttta acttgtgttt gttcattttt taataagagt cgagctaagt
     1801 cttgcaacat cattacttgc ttggcatcta actgttcgtc gttaaatttt atgaaacgat
     1861 cactcatgtt ttatccctct ttaaaagttt agttctacag cattatatat tgcttgttgt
     1921 tcacgttcat cttccaattt atcaataatt gtacgtttaa ttgcacgttc aactggcatt
     1981 acagtgatta aatcacacaa gtctaataat gcacggatac tagcggcttc ttcagaaatt
     2041 tgtccctgct tagacatagt acgtaaatct tcgttaaact taataatttg ttcgatttgt
     2101 ttatcatctt gtaataaact ttgctcttta atcacatttt ttaaaatgtc cccgtcaata
     2161 taatcaacgt gaataacaac aaagcgattt tttagtgctt cattcattgg caaagtacca
     2221 acataacctt catttatcgc tgctataacg ttaaatcctg gtacagcttt gattacttca
     2281 ccagtgtatg gattcgtaat ttgacgacga taatctaata caccatttaa tacaggcaat
     2341 gtttcaggtt tagccatatt tatttcatca atatataaaa tatgcccctc tttcatagct
     2401 ttaataactg gaccatctac aaagacaatt tcttgttgac cttccgcatt tgttttaatt
     2461 gttttaaagc ctaataagct ttctgtatct aaatcaacag aacaattgac ttgatgcatg
     2521 ggtgtatcaa caacttcact taatgtttct gccaactttg ttttccctga acctgttgga
     2581 cctttaagta aaatattttt atttaaatca aataatgcct tcgcatcatt gaaaactgtt
     2641 gaatctgaat tcttataatg ttttagtgcc attttaaaac gttctccttt aaataaatta
     2701 aaaaactaag acatggaata agttcttcaa atgccttagt ttactattct attctgatat
     2761 aaaacgcaat acttagtatg cgaattgttt atattttacc aaatttacta attgaaaatc
     2821 tcatattagg cttcatttca ataattatgc gtttaaactt ctttgaaata tgttttatag
     2881 taaccgccaa ctaatccaga attatctatt atttgataat attcttcagt ttcgttgata
     2941 acatcatatt gtttgccaac cgttaacatg tcagaaactg taaatttctt tgcatctgta
     3001 ttaatgactt ctactttctt gattggtgta cgttctttcc aagtttcatg taacataaat
     3061 tttgtcctca ctatcgtaaa aaaattattt ttgtaaagtg aatataaacg ataatccact
     3121 gttttcagta attctaattg attcaatatc atcttgctca agcgtttgat atggtatttc
     3181 cacttcatct aagcgcgatt tcgcttgatt aaagaaatct gcatctttcg tttcaaactc
     3241 aacttgatcg actgcgccat attccggcat tgtcatttct gtttctacag gcattaaatg
     3301 tatttcgcca cctagaccac ccgttccaac tttgaatact tggacatgat agtcagcatt
     3361 gtcgaagggt tggtattctg caaatacttc aagtccgaat atatttgtta aaatttgacc
     3421 tgtaatatct acatgattca ctttaagaat cactggtcct aaaccttgca cttgatgtaa
     3481 cggattgacc gcactctcaa aagaaggcat acctaaacca acgccataat tattctcgtt
     3541 tgaatatatt gagaaaatat gaccattgtt atcttcgaga ctgaaatatt tattgccatt
     3601 taattcttta actgttgtaa atggaatatc cttattcgat aatatttccg catactcctc
     3661 taaacctgag tcagttggtg tacgtaatcc aatacccaca aagtgagatt cttctaattg
     3721 ctcacttgga acttgtataa attgaagtct tgttcctggg cttatatctg catcgccgaa
     3781 acgaattgag ttttcaagaa gttcttcata atttaatcct aatatgtcaa ccatgaaatg
     3841 ttttgtctgt tctatatttg ttgtacctaa tgttatactt ctaagtccac acattatagc
     3901 cacctct
//
LOCUS       DANBWH010000212         3905 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.220, whole genome shotgun sequence.
ACCESSION   DANBWH010000212 DANBWH010000000
VERSION     DANBWH010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.220"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..272)
                     /locus_tag="PPY92_002372"
     CDS             complement(<1..272)
                     /locus_tag="PPY92_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HDJ2626347.1"
                     /translation="MKWFKLILDVTTFILIAILLFVYTYKENEEILPDTKYPIAVTDW
                     NKKYSKNEIYKRINQFAKNENVAIYKSTSNYTNKNVDKDIYVFNKS"
     gene            complement(316..588)
                     /locus_tag="PPY92_002373"
     CDS             complement(316..588)
                     /locus_tag="PPY92_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HDJ2626348.1"
                     /translation="MFGTILYLTLALGLSTAAYASTEYAEGGTWSHGVGSKYVWSYYY
                     HGHKGHGATAIGKYRSFSGYTRAGVKAKASATKHNCWVNRAYYNIY"
     gene            complement(598..699)
                     /locus_tag="PPY92_002374"
     CDS             complement(598..699)
                     /locus_tag="PPY92_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626349.1"
                     /translation="MKFVANKLTLTIIRKFCINNEKEIWIKYEEVFL"
     gene            complement(1238..1383)
                     /locus_tag="PPY92_002375"
                     /pseudo
     CDS             complement(1238..1383)
                     /locus_tag="PPY92_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500662.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
     gene            complement(1615..2217)
                     /locus_tag="PPY92_002376"
     CDS             complement(1615..2217)
                     /locus_tag="PPY92_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDJ2626350.1"
                     /translation="MNKISKALTWFIISFIIFHLILFIMWGEHQEYWYLYTGIMLIAG
                     ISYVFYQRDIESKRLLTSIGVGIITAIILIMLQLLFSLITSNLSYSSLIKELARTGVN
                     WKWQMLVTLLFVIPCHELYMRTVLQKELTHFSIPKWIAILITAICSSSLFIYLDNWWI
                     VTFIFVAQVILSLSYEYTRRIATTSVAQIVAIILLLIFNA"
     gene            complement(2232..2408)
                     /locus_tag="PPY92_002377"
     CDS             complement(2232..2408)
                     /locus_tag="PPY92_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkvS family protein"
                     /protein_id="HDJ2626351.1"
                     /translation="MTVAEVGNIVEFMDGLRGRVEKINDNSVIVDLTIMENFNDLDLP
                     EKTVINHKRYKIVE"
     gene            2607..3593
                     /locus_tag="PPY92_002378"
     CDS             2607..3593
                     /locus_tag="PPY92_002378"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDJ2626352.1"
                     /translation="MKFISNNNITDPTLNLAMEEYVLKNLPAEESYFLFYINRPSIIV
                     GKNQNTIEEVNQTYIDAHNIDVVRRISGGGAVYHDTGNLNFSFITDDDGNSFHNFQKF
                     TEPIVQALQSLGVNAELTGRNDIQVGQAKISGNAMVKVKNRMFSHGTLMLNSDLDEVQ
                     NALKVNPAKIKSKGIKSVRKRVANIQEFLNDPLEIEEFKKIILKTIFGETEVEEYKLT
                     DEDWENIEKLSNDKYRTWAWNYGRNPKYNFEREEKFEKGFVQIKFDVKRGKIEHAKIF
                     GDFFGVGDVTDLENALVGCLHDFEHIEEALSEYDLYHYFGDIDRHELIRLMS"
     gene            complement(3674..3892)
                     /locus_tag="PPY92_002379"
     CDS             complement(3674..3892)
                     /locus_tag="PPY92_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HDJ2626353.1"
                     /translation="MKYNTNVKHTTLEAFVTTVNDLGIELIINEALREVRKRQLIELI
                     DDALVNKDEAAFNQYTAEYKNLEAFLGE"
BASE COUNT         1418 a          586 c          554 g         1347 t
ORIGIN      
        1 tttgatttat taaatacata tatatcttta tctacgtttt tgtttgtata atttgaagtt
       61 gatttataga ttgctacgtt ctcattttta gcgaattgat ttatacgttt ataaatctca
      121 tttttactat attttttatt ccagtcagtt accgctatag ggtatttagt atcaggcaat
      181 atttcttcgt tttctttata tgtataaaca aataacaata tagcaatcag aatgaaggtt
      241 gtaacatcaa gtattaattt gaaccatttc atgttgtacc tcttgcgttg tttattttct
      301 atgatttttt agtatttaat aaatgttata atacgctcta ttgacccagc aattatgttt
      361 agtagctgat gcttttgctt ttacaccagc tcttgtataa ccactaaatg atctatattt
      421 tccaatagct gttgcaccat gtcctttatg accatgataa taataagacc aaacatactt
      481 actgccgaca ccgtgactcc aagtgcctcc ttctgcgtat tctgtagatg cataagctgc
      541 tgttgataat ccaagtgcta aagttaaata taatatagtg ccaaaaattt tattcttcta
      601 taaaaacacc tcttcatatt taatccatat ctctttttcg ttatttatac agaattttct
      661 gataatagtc aaggttaatt tatttgctac aaatttcaac cactttatgt taaatggaca
      721 tcaaagtata attgtattat tgttaaattg cgcagtgcat aaatagacga catgcaggaa
      781 catgtcccct attgaacccg ttaaaaagac ggtgactaaa tgagattttc tttaaccatc
      841 attcgttgtc aaagttttga aatgatggtt gttttttatt gtttaaatta atcattgcga
      901 cttctatatc aatgaaagtt atattaatat atgaacttca ttacatattc aatagaaatc
      961 acttatttat aagtactacc atataatcat aattgatttc taccacagta tgattttcct
     1021 ttcaaatctg acatgtttag caggcaatat acggaaaata cttcaaaatt ttgttacaca
     1081 tctaataaaa atagacaatt aaataaaaga tatgttacta taaaaatgta aaaagacgac
     1141 atgcaggaac atgtcgccta atgagcccgt taaaaagacg gtgactaaat gagattttct
     1201 ttaaccatca ttctatgtca aagttttgaa atgatggtta ttttttattg cttaaattta
     1261 ttattgctac tactatacca atgaaagtga cgataaacat accgaaactt aacattaaat
     1321 gtaatgcgtt tgcaatagat atcacaatag gcttttcctt tcaaaagtgt taagtaatgc
     1381 cataggtacc accccccttt actcagatag ccaccatcaa ttcaacttgc ccacaaaata
     1441 ttatactatt tttataaaat atttagtaat aaaaactgac taaaatataa cctcaataat
     1501 aactgcttgc cgttaaaaat gtatacacta taataaagca tgccttgaat cttcgtattg
     1561 cttttctaaa gcaaaaagct tttcttagaa cacagacgtc taagaaaagc ttatttaagc
     1621 attaaaaatt aataacaata taatagcaac aatttgcgca acggatgttg ttgctatgcg
     1681 tcgagtatac tcataactta atgataatat cacttgtgca acaaatataa atgttactat
     1741 ccaccaatta tctaaataaa taaataatga actagaacaa attgcagtta ttaatatagc
     1801 tatccatttc ggtattgaaa aatgtgttaa ttctttttgt aaaacagttc tcatatataa
     1861 ctcgtgacat ggaatgacaa aaagtaaagt tactaacatt tgccacttcc aattgacacc
     1921 tgttcttgct aattctttaa ttaatgaact ataacttaaa ttagaagtta taagtgagaa
     1981 taaaagttga agcataatta aaataattgc cgtaataata ccaacaccaa ttgatgtaag
     2041 caaccgctta gattcaatat ctctttgata aaatacataa ctgataccag caattagcat
     2101 tatacctgta tataaatacc agtattcttg gtgttcgccc cacataataa ataatatgag
     2161 atgaaatata atgaaactta taataaacca agttaaagcc ttcgagattt tattcattat
     2221 aacttacctt cttattcaac aatcttatat cgtttatgat tgataacagt tttttccggt
     2281 aaatcaaggt cattaaaatt ttccataatt gttaagtcaa caataacaga gttatcgttg
     2341 attttttcaa cacgacctct taatccatcc ataaactcaa caatgttacc cacttctgca
     2401 acagtcattt taagtcctcc ttaaatcata actaaattat attgtactaa aattcactta
     2461 acaaataaac tattaacaat caaaaataat aaaatgtaac tttaagccga tatttagaat
     2521 gatttgtgag tatataattg gcattttgag caaagttaag gcataaataa gtagagaata
     2581 tatatagata taagggagag attgtaatga aattcattag taataataat attacagatc
     2641 caactttaaa cttagcaatg gaagaatatg ttttaaaaaa tttaccagca gaagaaagtt
     2701 actttttatt ttacataaat agaccatcta tcattgttgg aaagaatcaa aatacgatag
     2761 aggaagtaaa tcagacatat atagatgctc ataacatcga tgtagttaga agaatttctg
     2821 gtggtggcgc tgtttatcat gatactggca atttaaactt tagttttata acagatgatg
     2881 atggtaacag tttccacaat ttccaaaaat tcactgaacc aattgttcaa gcattacaat
     2941 ctttaggcgt aaatgctgaa ttaactggtc gtaacgatat tcaagttgga caagcgaaaa
     3001 tctcagggaa tgctatggtt aaagttaaaa atagaatgtt tagtcatggt acgttgatgt
     3061 taaatagtga tttagacgaa gttcagaatg cactaaaagt taatccagct aagattaaat
     3121 caaaaggtat taaatctgtt cgtaaacgag tagcaaacat tcaagaattt ttaaatgacc
     3181 ctttagaaat tgaagaattt aaaaaaatca tcttgaaaac tatctttgga gaaactgaag
     3241 tagaagaata taaattaacg gatgaagatt gggaaaatat tgaaaagtta agtaacgaca
     3301 aatatagaac ttgggcatgg aattatggta gaaaccctaa atataacttt gaacgcgagg
     3361 aaaaatttga aaaaggattt gtacaaatta agtttgatgt taaacgaggt aaaatcgaac
     3421 atgcaaaaat tttcggtgat ttctttggtg tcggagatgt cactgatctt gaaaatgcat
     3481 tagttggctg tctacacgac tttgagcaca tagaagaagc actatcagaa tatgatttat
     3541 atcattattt cggtgacata gatagacatg aattaattag attaatgtca taaacgatta
     3601 aaggactatt cttacatgta tgagatgtta agaatagtcc ttttatgatt gcgttatgga
     3661 attcgaatca atgttattca ccgagaaatg cctccaaatt tttgtattct gccgtatatt
     3721 gattaaatgc tgcttcatct ttattgacga gtgcgtcatc tataagttct atgagctgtc
     3781 gttttcttac ctctcgaagt gcttcattga taattaattc aatacccaaa tcattgacag
     3841 ttgtgacaaa cgcttctaaa gttgtatgtt taacattagt attgtatttc atagaaacca
     3901 ctacc
//
LOCUS       DANBWH010000213         3863 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.221, whole genome shotgun sequence.
ACCESSION   DANBWH010000213 DANBWH010000000
VERSION     DANBWH010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.221"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            73..291
                     /locus_tag="PPY92_002380"
     CDS             73..291
                     /locus_tag="PPY92_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDJ2626354.1"
                     /translation="MTKLAYPMLYITRKEKGDTQKKVASKLGISPQRYQLKESGKAIF
                     NLNECQILSEMYDMPIDELFSSKIKVGE"
     gene            303..449
                     /locus_tag="PPY92_002381"
     CDS             303..449
                     /locus_tag="PPY92_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626355.1"
                     /translation="MKLNLFYITLTSLLTILLLAISNMYVAFSVYGMMVIYGFNLTGG
                     LENE"
     gene            442..666
                     /locus_tag="PPY92_002382"
     CDS             442..666
                     /locus_tag="PPY92_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626356.1"
                     /translation="MNNEQKEVIQDIYNTLEAVAYNTSMEYIHNCVDGKKEWMENVNR
                     EEHLQAIIEWALQQIENNFDFENDTEMEEL"
     gene            667..966
                     /locus_tag="PPY92_002383"
     CDS             667..966
                     /locus_tag="PPY92_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1474 family protein"
                     /protein_id="HDJ2626357.1"
                     /translation="MNWEIKNLMCDLKILKEKFEDLKDNHGSHFEDLYLHEPNHTLNK
                     DDVIKEGFSYHERRIHNDQMFDLLLLYTEKFDELITRFDALEKASSFADQSQDNA"
     gene            1059..3431
                     /locus_tag="PPY92_002384"
     CDS             1059..3431
                     /locus_tag="PPY92_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDJ2626358.1"
                     /translation="MAIKKKDKIIGVKELEIPHEMKLVPNWVLWRAEWNEKQQNFGKV
                     PYSINGYRASTTNKKTWCDFESVSIEYEVDEQYSGIGFVLSDGNNFVCLDIDNAIDKK
                     GQINSELALKMMQLTYCEKSPSGTGLHCFFKGKLPDNRKKKRTDLEIELYDSARFMTV
                     TGCTIGQSDICDNQEVLNTLVDEYFKENLPVNDIVREESNTNMQLSDEDIINIMMKSK
                     QKDKIKDLLQGTYESYFESSSEAVQSLLHYLAFYTGKNKQQMERIFLNYNNLTDKWES
                     KRGNTTWGQLELDKAIKNQKTVYTKSIDEFNVIQQGSKDIKQLLNQLGHEERTKMEEK
                     WIEGGKRGRKPTTISPIKCAYILNEHLTFILFDDEENTKLAMYQFDEGIYTQNTTIIK
                     RVISYLEPKHNSNKADEVIYHLTNMVDIKEKTNSPYLIPVKNGVFNRKTKQLESFTPD
                     YIFTTKIDTSYVRQDIVPEINGWNIDRWIEEIACNDNQVVKLLWQVINDSMNGNYTRK
                     KAIFLVGKGNNGKGTFQELLSNVIGYSNIASLKVNEFDERFKLSVLEGKTAVIGDDVP
                     VGVYVDDSSNFKSVVTGDPVLVEFKNKPLYRATFKCTVIQSTNGMPKFKDKTGGTLRR
                     LLIVPFNANFNGIKENFKIKEDYIKNPQVLEYVLYKAINLDFETFDIPDASEKMLEVF
                     KEDNDPVYGFKVNMFDQWTIRKVPKYIVYAFYKEYCDENGYNALSSNKFYKQFEHYLE
                     NYWKTDAQRRYDNEELAKRIYNFNDNRNYIEPIESGKNYKSYEKVKLKAI"
BASE COUNT         1560 a          464 c          706 g         1133 t
ORIGIN      
        1 ggatataaca actatataat gtatttgtta atttaaataa caagcgaact tgttatttta
       61 ggaggtgttc gcatgacaaa actagcttat ccaatgttat acatcactag aaaagaaaaa
      121 ggtgacacac aaaagaaagt tgctagcaaa cttggtatta gtccacaacg ttaccagtta
      181 aaggaaagtg gtaaagcaat atttaattta aatgagtgtc agattctttc agaaatgtat
      241 gatatgccaa ttgatgagtt attttcatca aaaattaaag taggagaata ggaagcaaca
      301 caatgaaatt gaatttattt tatataactt taacatcgct gttaacaata ttattactag
      361 caatatctaa catgtatgtc gcttttagtg tgtacggcat gatggtaatt tatggattta
      421 atttaacagg aggattagaa aatgaataat gaacaaaaag aagttattca agacatctat
      481 aatactttag aagcagtagc ttataacaca tcaatggaat acatccataa ttgcgtagat
      541 ggtaaaaaag aatggatgga aaacgtaaat cgtgaggaac acttacaagc cataatcgag
      601 tgggcattac aacaaataga aaataacttt gattttgaga atgatactga aatggaggaa
      661 ttataaatga attgggaaat taaaaattta atgtgtgatt taaagatatt gaaagaaaag
      721 tttgaggatt taaaggataa tcatggctcg cattttgaag atttatatct acatgagcca
      781 aatcatacct taaataaaga cgatgttatt aaagaaggat tttcatatca tgagagacgt
      841 attcacaatg atcaaatgtt tgatttgcta cttctataca ctgaaaaatt cgatgaatta
      901 ataacgaggt ttgatgcatt agaaaaagcg tcatctttcg ccgaccaaag ccaagataac
      961 gcataaaaaa aataaaaatt tataaaatca taccattaat attttaacat tagttgcatt
     1021 gaaaatacag agaaatcaga ttagaaagga ggcattctgt ggcaattaag aaaaaagata
     1081 aaattattgg agttaaagaa ttagaaatac ctcatgaaat gaagttagtg cccaattggg
     1141 tattatggcg agctgaatgg aacgagaagc aacaaaattt tggaaaagta ccatatagta
     1201 ttaatggtta cagagctagt acaaccaata aaaaaacatg gtgtgacttt gaaagtgtaa
     1261 gtattgaata tgaagttgat gagcaatata gcggtatagg ttttgtatta agtgatggta
     1321 ataattttgt ttgcctagat attgataatg caattgataa aaaaggacaa atcaattctg
     1381 aattagcact aaaaatgatg caactcacat attgtgaaaa gtctccaagt ggtacaggat
     1441 tacattgttt ctttaaaggt aaactaccag ataaccgtaa aaagaaaaga acggatttag
     1501 agatagagtt atatgattca gcaaggttta tgactgttac aggatgcaca attggccaaa
     1561 gtgatatttg tgataatcaa gaagtattaa atactctcgt tgatgaatac tttaaagaga
     1621 atttgccagt aaatgatatt gtgagagagg aatctaatac caatatgcaa ttatctgatg
     1681 aagatattat aaacattatg atgaaatcta aacaaaaaga taaaattaaa gatctattac
     1741 aaggcacata tgaatcatat tttgagagtt caagcgaagc agtacaaagc ttattacact
     1801 acttagcgtt ttacacaggt aaaaacaaac agcaaatgga gcgtatattt ttaaactaca
     1861 ataatcttac ggataaatgg gaaagtaaac gaggtaatac gacttgggga cagcttgagt
     1921 tagataaagc tataaagaat caaaagacag tttacactaa atccatagat gaatttaatg
     1981 ttatacaaca ggggagtaaa gatattaaac agttattgaa tcaattgggg catgaagaaa
     2041 gaacaaaaat ggaagaaaag tggattgaag gaggaaaacg agggcgaaag cctacaacaa
     2101 ttagccctat aaaatgtgca tatattttga atgagcattt aacatttata ctttttgatg
     2161 atgaagaaaa tactaagtta gctatgtatc aatttgatga agggatatat acacagaaca
     2221 ctacaattat aaaacgagta atttcctatt tagagcccaa acataatagt aataaagctg
     2281 atgaagttat ttatcattta accaatatgg tagatataaa agagaaaact aactcaccat
     2341 acttaatacc agttaaaaat ggtgtattta accgtaaaac gaagcaacta gaatcattta
     2401 cacctgatta tatatttacc actaaaatag atacatcgta tgtaaggcaa gatatagtac
     2461 ctgaaataaa tggctggaat atagatcggt ggatagaaga aatagcttgt aatgataatc
     2521 aggttgttaa attattgtgg caagtaatta atgactcaat gaatggaaac tacacacgta
     2581 aaaaagcaat atttttggtt ggtaagggta acaatggtaa aggtacattt caagaattat
     2641 tgtctaatgt aataggttat agcaatattg ctagcttaaa agtgaatgag tttgatgaac
     2701 gttttaaatt gagtgtgtta gagggcaaga cagcagtaat tggcgacgat gtaccagttg
     2761 gtgtgtatgt cgatgattct tcaaacttta aaagtgtagt tactggcgat ccagtgttgg
     2821 ttgagtttaa aaataaaccc ttatatagag cgacttttaa gtgtacagtt attcaatcaa
     2881 cgaatggaat gcctaaattt aaagacaaaa caggcgggac tttaagaagg ttattgatag
     2941 taccgtttaa tgccaatttt aatggcatta aagagaattt taaaatcaaa gaagactata
     3001 taaaaaatcc gcaagtgcta gagtatgtgc tttataaagc aattaattta gattttgaaa
     3061 cttttgacat tcctgatgca tctgaaaaaa tgcttgaagt atttaaagaa gataacgatc
     3121 cagtttatgg gtttaaagta aatatgtttg atcaatggac tattagaaaa gtgccgaaat
     3181 atattgtata cgcattttat aaagaatatt gtgatgaaaa tggctataat gcattgagtt
     3241 caaacaagtt ttataaacaa tttgaacatt atttagagaa ttattggaaa actgatgcac
     3301 agcgaagata tgacaatgaa gaacttgcta agaggatata caactttaat gacaatagaa
     3361 attacattga acctattgaa agtggaaaaa actataaatc gtatgaaaag gtgaagctaa
     3421 aagcaatata ggtgcaaccg aatgcaaccg aatgcaaccg aaaattttaa aacgggttgc
     3481 acctataacc acacttgtat caaggggtta gcgtgtttat gcaaccgagc aactcaaaat
     3541 ctataactta ttagcagaaa agtaaataac caaaatataa ataagaaata gtatccaaaa
     3601 ttatataaac taatttgtgt gatttcggtt gcatagttgc aaaagtggat taaccccttg
     3661 tgacagtaag aaaaaaattg caactggaaa atttaaaacg agttgcatag ctgtaaattt
     3721 cagttgcaca taatcggaag agacttgcag ttatacaaaa ttgcctaaga tactaaagtt
     3781 actaaaaaac gtttaagtca ctaaaagata ctaaaaattt aaattttagt aaccacagtt
     3841 tttactttca tattaggctt ttt
//
LOCUS       DANBWH010000214         3824 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.222, whole genome shotgun sequence.
ACCESSION   DANBWH010000214 DANBWH010000000
VERSION     DANBWH010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.222"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..790)
                     /locus_tag="PPY92_002385"
     CDS             complement(<1..790)
                     /locus_tag="PPY92_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDJ2626359.1"
                     /translation="MSYTSVLFNKNFTFYLIGIAFSNMGSAFTTFVFPLMILKLTGSA
                     FQVGIVSALSFIPYAILGLPAGALIDRLNKKTIMKCADIIRFVSYLSIPVLSFFNMLS
                     IFQIYVVAIISGIGLVFHSISEVSIIPSIVKEEDLASANSYIYATQNVSEFLGPIIGG
                     LLYTYMGYSILIFIDSMTFLLSFFSLILIKIETKSIFNQEKLSGKNFLSDVKVGFDYL
                     LSNSTIRVMAVVVSLSNLIISPYYIYIVMFVKEDMNQSSQALGLV"
     gene            complement(1106..2512)
                     /gene="gndA"
                     /locus_tag="PPY92_002386"
     CDS             complement(1106..2512)
                     /gene="gndA"
                     /locus_tag="PPY92_002386"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDJ2626360.1"
                     /translation="MTQQIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEE
                     SKGKNIHPTYSLEEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNT
                     NYQDTIRRNKALAQSAINFIGMGVSGGEIGALTGPSLMPGGQEEAYNKVADILDAIAA
                     KAKDGASCVTYIGPNGAGHYVKMVHNGIEYADMQLIAESYAMMKELLGMSHEDIAQTF
                     KDWNAGELESYLIEITGDIFMKLDENKEALVEKILDTAGQKGTGKWTSINALELGIPL
                     TIITESVFARFISSIKEERVNASKELNGPKASFDGDKKDFLEKIRKALYMSKICSYAQ
                     GFAQMRKASEDNEWNLKLGDLAMIWREGCIIRAQFLQKIKDAYDNNPGLQNLLLDPYF
                     KNIVTEYQDALRDVVATGVQNGVPTPGFSSSINYYDSYRAADLPANLIQAQRDYFGAH
                     TYERKDKEGVFHTQWIEE"
     gene            complement(2580..3713)
                     /locus_tag="PPY92_002387"
     CDS             complement(2580..3713)
                     /locus_tag="PPY92_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDJ2626361.1"
                     /translation="MINEQRLLNTFLELVQIDSETGNESTIQPILKEKFIALGLDVKE
                     DEAAKHPKLGANNLVCTMNSTIEEGEVPKLYLTSHMDTVVPAINVKPIVKDDGYIYSD
                     GTTILGADDKAGLAAMLEVLQVIKEQQIPHGQIQFVITVGEESGLIGAKELNSELLDA
                     DFGYAIDASADVGTTVVGAPTQMLISAKIIGKTAHASTPKEGVSAINIAAKAISRMKL
                     GQVDEITTANIGKFHGGSATNIVADEVILEAEARSHDPERIKTQVKHMTDVFETTASE
                     LGGKAEVTVEQSYPGFKINDNEAVVKIAQESARNLGLSANTIISGGGSDGSIINTFGI
                     PSVILGVGYEKIHTTNERMPIKSLNLLASQVLEIIKIVARQSK"
BASE COUNT         1265 a          731 c          514 g         1314 t
ORIGIN      
        1 aaactaaccc caatgcttga gatgattgat tcatgtcttc ctttacaaac attactatat
       61 aaatataata aggtgagata attagattgg ataaacttac aacaacagcc attactctaa
      121 tagttgaatt agataataga taatcaaatc caactttaac atcgctcaaa aaattttttc
      181 cactcaactt ttcttgattg aatatactct ttgtttcaat tttaattaat atcaaactga
      241 aaaatgagag taaaaaagtc atagaatcta taaaaatcaa tattgaatat cccatatatg
      301 tataaagtaa acctccaatt atagggccca aaaattccga aacattttga gtagcgtata
      361 tataactgtt tgcgctcgcc aaatcttctt cttttacaat acttgggatt atcgaaacct
      421 cagaaatact gtgaaatact aatccaatac ctgaaatgat tgcaactaca taaatttgaa
      481 agatgcttaa catattgaaa aaacttaata ctggtatact taaatatgaa acgaacctaa
      541 ttatatcagc acatttcatt atggtcttct tattcaatct gtcaattaaa gcaccagctg
      601 gcaatcctaa aattgcataa ggtataaaac ttagtgcaga aactatgcca acttgaaatg
      661 cagatccagt aagctttaaa atcattaatg gaaaaacaaa cgtagtaaaa gcactgccca
      721 tgttagaaaa agctatccct attaaataaa aagtaaagtt cttattaaac aatacagaag
      781 tatatgacat tctatccctc ttattcatca aataataaaa cgtaatctat ttatcgaaat
      841 attaaaatta tttactaatg ctatcttaat agtatgtaag taaaacgtct acacttaaac
      901 ggacaaattc tatgaggata gatatcgtta atttataaaa gttgaaaatt ttaatttgac
      961 aagagaaaga aaatcattga atatatcatg cgtagaaaat ttcgataaat acataaggaa
     1021 agagacaatt tctatcaaat aaataataga aattgtctct ttaacattac ctaaattcat
     1081 aaggtctcag gctcaatggt caatattatt cttcaatcca ttgtgtatgg aatacgcctt
     1141 ctttatcttt tctttcgtac gtgtgagcac cgaagtagtc acgttgtgct tgaattaagt
     1201 ttgcaggtaa atcagctgca cggtaactat catagtaatt aatacttgat gagaaaccag
     1261 gtgttggtac accattttga acaccagttg cgacaacatc acgtaacgca tcttgatatt
     1321 cagtaacgat gtttttaaag taaggatcta gcaataagtt ttgtaatcct ggattattat
     1381 cgtaagcatc tttgatcttt tgtaagaatt gtgcacgtat aatgcaacct tctctccaaa
     1441 tcatagctaa atcaccaagt tttaaattcc attcattatc ttcacttgct ttacgcattt
     1501 gcgcgaaacc ttgtgcataa gaacaaattt tactcatata taatgcttta cgaatttttt
     1561 ctaaaaagtc tttcttgtca ccatcaaatg atgcttttgg accatttaat tctttagaag
     1621 catttacgcg ctcttctttg attgaagaga taaaacgtgc aaatacagat tcagtaatga
     1681 ttgttaatgg aatacctaat tctaatgcat taattgaagt ccattttcct gtaccttttt
     1741 gacctgcagt atcaagaatt ttttcaacta atgcttcttt attttcatct aatttcatga
     1801 aaatatcacc agtgatttca attaaataac tttctaattc accagcattc cagtctttga
     1861 acgtttgagc aatgtcttca tgagacatac ctaataattc tttcatcata gcataacttt
     1921 ctgcaattaa ttgcatgtca gcatattcaa taccattatg taccattttc acatagtgtc
     1981 cagcaccatt tggtccaata taagtaacac atgaagcacc gtcttttgcc ttagcagcaa
     2041 ttgcatcaag aatatctgca actttgttat aagcttcttc ttgtccaccc ggcattaatg
     2101 acggaccagt taacgctcca atttcaccac cagaaacgcc cataccaata aagttgattg
     2161 cactttgtgc taatgcttta ttacgtctga tagtatcttg atagtttgta ttaccaccat
     2221 caattaaaat atctccatca tctaataaag gtaacaaact atcaattgtt gcatccgtag
     2281 ctttacctgc ttgaaccatt aataaaattt tacgtggttt ttctaaagaa ttaacaaatt
     2341 cttccaatga atacgttgga tgaatatttt tcccttttga ttcttcaacc attaaatcag
     2401 ttttttcact tgagcggtta aatacagata cactatatcc gcgtgattca atattccaag
     2461 ctaggttttt acccataacg gctaaaccaa taactccaat ttgttgtgtc atattactta
     2521 cctcacttgt tgatttttca ttagtattgt atcacaaaat agacatacac tacactaaat
     2581 catttcgatt gtcgcgcaac tattttgatt atttctaaca cttgacttgc aagcaagttc
     2641 aatgatttaa tcggcattct ctcatttgtt gtatggattt tttcataacc cactcctaaa
     2701 atgactgaag gaataccaaa tgtattaata atactgccgt ctgaaccgcc accagaaata
     2761 attgtatttg cagataatcc taaattacga gcactttctt gtgcaatttt aacaaccgct
     2821 tcattatcat taattttaaa tcctggataa ctttgctcca ctgtaacttc tgctttccca
     2881 cctaattctg atgcagtagt ttcaaacaca tcagtcatat gtttgacttg tgtttttatt
     2941 ctttctggat cgtgagaacg tgcctctgct tctaaaatga cttcatctgc aacaatattc
     3001 gtagctgaac cgccatgaaa cttaccaata ttggcagtag ttatttcatc aacttgtcct
     3061 aatttcattc gactaattgc tttagccgca atattaatag cactaacacc ctcttttggc
     3121 gtacttgcat gagctgtttt gccaataatt ttagctgaaa ttaacatttg cgtcggtgca
     3181 cctacaaccg tagtaccgac atcagcactt gcatcaatag cataaccaaa gtccgcgtcc
     3241 aacaactctg aatttaattc tttagcacca attaaacctg attcttcccc aacagtaatc
     3301 acaaattgaa tttgtccatg tgggatttgt tgttccttta tcacttgcaa aacttcaagc
     3361 atcgctgcta atcctgcttt atcatctgca cctagaatag tcgtaccatc agagtatatg
     3421 tagccgtcat cttttacaat tggctttaca ttaattgcgg gtacaacagt atccatatgg
     3481 ctcgtcaaat ataatttagg tacttcgcct tcttcgatag tactattcat tgtacacact
     3541 agattattgg cacctaattt aggatgttta gccgcttcat cttctttaac atctaaccct
     3601 aatgctatga atttttcttt taaaataggt tggattgttg attcattccc tgtctcagaa
     3661 tcgatttgta caagttctaa aaacgtatta agtaatcttt gctcattaat cataatggaa
     3721 ccccttttat caaaattttc aagtacgcca aacggtaaaa attgatgaaa taaaatgtta
     3781 cagtaattga cgttacacag atttatcagg cttgtaaatt gtgt
//
LOCUS       DANBWH010000215         3786 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.223, whole genome shotgun sequence.
ACCESSION   DANBWH010000215 DANBWH010000000
VERSION     DANBWH010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.223"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            66..2141
                     /gene="topA"
                     /locus_tag="PPY92_002388"
     CDS             66..2141
                     /gene="topA"
                     /locus_tag="PPY92_002388"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDJ2626362.1"
                     /translation="MTLADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQM
                     GVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILEL
                     EDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKV
                     KKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPF
                     KLKTKKDVEKITAALDGDQFEITNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKT
                     MMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKAEAKQYITDKYGESYTSKRKA
                     SGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVS
                     LDITQGDIKFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPA
                     QHYTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFVPTELGEI
                     VHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEEEME
                     KIEIKDEPAGEDCEVCGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCND
                     GDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSN
                     CDYKEAAQK"
     gene            2297..3604
                     /gene="trmFO"
                     /locus_tag="PPY92_002389"
     CDS             2297..3604
                     /gene="trmFO"
                     /locus_tag="PPY92_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HDJ2626363.1"
                     /translation="MTQTVNVIGAGLAGSEAAYQLAERGIKVNLIEMRPVKQTPAHHT
                     DKFAELVCSNSLRGNALTNGVGVLKEEMRRLNSIIIEAADKARVPAGGALAVDRHDFS
                     GYITETLKNHENITVINEEINAIPDGYTIIATGPLTTETLAQEIVDITGKDQLYFYDA
                     AAPIIEKESIDMDKVYLKSRYDKGEAAYLNCPMTEDEFNRFYDAVLEAEVAPVNSFEK
                     EKYFEGCMPFEVMAERGRKTLLFGPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNI
                     VGFQTHLKWGAQKEVIKLIPGLENVDIVRYGVMHRNTFINSPDVLNEKYELISQPNIQ
                     FAGQMTGVEGYVESAASGLVAGINLAHKILGKGEVVFPRETMIGSMAYYISHAKNNKN
                     FQPMNANFGLLPSLETRIKDKKERYEAQANRALDYLENFKKTL"
BASE COUNT         1451 a          563 c          733 g         1039 t
ORIGIN      
        1 acgttgacaa aagcaaaatt aagcgtttat catttatctt tagtaattag attagcgagg
       61 gggaaatgac attggcagat aatttagtca ttgttgaatc gcctgcaaaa gcaaaaacca
      121 ttgaaaagta tttaggtaag aaatataaag ttatagcttc aatgggacac gtcagagact
      181 taccaagaag tcaaatgggt gtcgacactg aagataatta cgaaccaaaa tatataacaa
      241 tacgcggaaa aggtcctgtt gtaaaagaat tgaaaaaaca tgcaaaaaaa gcgaaaaacg
      301 tctttctcgc aagtgacccc gaccgtgaag gtgaagcaat tgcttggcat ttatcaaaaa
      361 ttttagagct tgaagattct aaagaaaatc gcgttgtttt caacgaaata actaaagacg
      421 ctgttaaaga aagttttaaa aatcctagag aaattgaaat gaacttagtc gatgcacaac
      481 aagcgcgtcg aatattagat agattggttg gctataacat ctcgccagtt ctatggaaaa
      541 aagtgaaaaa aggtttgtca gcgggtcgag ttcaatctgt tgcacttcgt ttagtcattg
      601 accgtgaaaa tgaaattcga aactttaaac cagaagaata ttggactatt gaaggagaat
      661 ttagatacaa aaaatcaaaa ttcaatgcta aattccttca ttataaaaat aaacctttta
      721 aattaaaaac gaaaaaagat gttgagaaaa ttacagctgc actagatgga gatcaattcg
      781 aaattacaaa cgtgactaaa aaagaaaaaa cgcgtaatcc agcaaaccca tttacaactt
      841 ctacattaca acaagaggcg gcacgtaaat taaactttaa agcaagaaaa acaatgatgg
      901 tcgcacaaca attatatgaa ggtatagatt tgaaaaaaca aggtacgatt ggtttaataa
      961 catatatgag aaccgattct acacgtattt cagatactgc caaagctgaa gcaaaacagt
     1021 atataactga taaatacggt gaatcttaca cttctaaacg taaagcatca gggaaacaag
     1081 gtgaccaaga tgcccatgag gctattagac cttcaagtac tatgcgtacg ccagatgata
     1141 tgaagtcatt tttgacgaaa gaccaatacc gattatacaa attaatttgg gaacgatttg
     1201 ttgctagtca aatggctcca gcaatacttg atacagtctc attagacata acacaaggtg
     1261 acattaaatt tagagcgaat ggtcaaacaa tcaagtttaa aggatttatg acactttatg
     1321 tagaaactaa agatgatagt gatagcgaaa aggaaaataa actgcctaaa ttagagcaag
     1381 gtgataaagt cacagcaact caaattgaac cagctcaaca ctatacacaa ccacctccaa
     1441 gatatactga ggcgagatta gtaaaaacac tagaagaatt gaaaattggg cgaccatcaa
     1501 cttatgcacc gacaatagat acgattcaaa agcgtaacta tgtcaaatta gaaagtaagc
     1561 gttttgttcc tactgagttg ggagaaatag ttcatgaaca agtgaaagaa tacttcccag
     1621 agattattga tgtggaattc acagtgaata tggaaacgtt acttgataag attgcagaag
     1681 gcgacattac atggagaaaa gtaatagacg gtttctttag tagctttaaa caagatgttg
     1741 aacgtgctga agaagagatg gaaaagattg aaatcaaaga tgagccagcc ggtgaagact
     1801 gtgaagtttg tggttctcct atggttataa aaatgggacg ctatggtaag ttcatggctt
     1861 gctcaaactt cccggattgt cgtaatacaa aagcgatagt taagtctatt ggtgttaaat
     1921 gtccaaaatg taatgatggt gacgtcgtag aaagaaaatc taaaaagaat cgtgtctttt
     1981 atggatgttc gaaatatcct gaatgcgact ttatctcttg ggataagccg attggaagag
     2041 attgtccaaa atgtaaccaa tatcttgttg aaaataaaaa aggcaagaca acacaagtaa
     2101 tatgttcaaa ttgcgattat aaagaggcag cgcagaaata atatttttat tccctagaga
     2161 cattttaaga ttgttaaata gaatcattag tgaatcttat tttaaagata gtaatggatt
     2221 aatctaaata agtgcggata atataaacat aacaacataa ttaaaagaca taaatgacaa
     2281 taaaaggagt atagaaatga ctcaaactgt aaatgtaata ggtgctggtc ttgccggttc
     2341 agaagcggca tatcaattag ctgaaagagg aattaaagtt aatctaatag agatgagacc
     2401 tgttaaacaa acaccagcgc accatactga taaatttgcg gaacttgtat gttccaattc
     2461 attacgcgga aatgctttaa ctaatggtgt gggtgtttta aaagaagaaa tgagaagatt
     2521 gaattctata attattgaag cggctgataa ggcacgagtt ccagctggtg gtgcattagc
     2581 agttgataga cacgattttt caggttatat tactgaaaca cttaaaaatc atgaaaatat
     2641 cacagttatt aatgaagaaa ttaatgccat tccagatgga tacacaatta tcgcaacagg
     2701 accacttact acagaaaccc ttgcgcaaga aatagtggac attactggta aagatcaact
     2761 ttatttctat gatgcagctg ctccaattat tgaaaaagaa tctattgata tggataaagt
     2821 ttacttaaag tcccgttatg ataaaggtga agctgcatat ttaaactgtc ctatgactga
     2881 ggatgaattt aatcgctttt atgatgcagt attagaagct gaagttgcac ctgtaaattc
     2941 atttgaaaaa gaaaaatatt tcgagggttg tatgcctttt gaagtaatgg cagaacgcgg
     3001 gcgcaagaca ttactatttg gaccaatgaa accagtagga ttagaagatc caaagactgg
     3061 gaaacgtcct tatgcagtgg ttcaattaag acaagatgac gctgctggca cactctacaa
     3121 tattgtcggc ttccaaacac atttaaaatg gggagctcaa aaagaagtca ttaaattaat
     3181 tccaggttta gagaatgttg atattgttag atatggtgtg atgcatagaa ataccttcat
     3241 taactcaccg gacgtattaa acgaaaaata tgaattgatt tcacaaccaa acatacagtt
     3301 tgcgggacaa atgactggtg ttgaaggtta tgtagaaagc gcagctagcg gcttagttgc
     3361 aggtatcaat cttgcgcata aaatattagg caaaggtgag gtagtatttc cgagagaaac
     3421 tatgattgga agtatggctt actatatttc tcatgctaaa aacaataaga atttccaacc
     3481 tatgaatgct aacttcgggt tattaccatc tttagaaact agaattaaag ataaaaaaga
     3541 acgctatgaa gcacaagcta atagagcttt ggattactta gaaaatttca aaaaaacttt
     3601 ataaaatagt tagaaagact agatatgcta ttcattctta agtcatcaac gagtaagtaa
     3661 tgactttcta aatggaaaat acttatccta gtctttttaa ttttggaatt gttacgtatt
     3721 tctgacaatt tagaattcgt attcaaaaaa atatctaaat aaataacacg caataagttg
     3781 attgat
//
LOCUS       DANBWH010000216         3748 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.224, whole genome shotgun sequence.
ACCESSION   DANBWH010000216 DANBWH010000000
VERSION     DANBWH010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.224"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            219..869
                     /locus_tag="PPY92_002390"
     CDS             219..869
                     /locus_tag="PPY92_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001795210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HDJ2626364.1"
                     /translation="MKITYKYRGDLPLNTENNKNQNQSVKNSERRGMLKGCGGCLISF
                     ILLIILLSACSMMFSNNDNSTSNQSSKTQLTQKDENKNEDKPEEKSETATDEDLQSTE
                     EVPANENTENNQHEIDEITTKDQSDDEINTPNVAEDKSQDDLKDDLKEKQQSSNHHQS
                     TQPKTSPSTETNRQQSFANCKQLRQVYPNGLTADHPAYRPHLDRDKDNRACEPDKN"
     gene            950..1945
                     /locus_tag="PPY92_002391"
     CDS             950..1945
                     /locus_tag="PPY92_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDJ2626365.1"
                     /translation="MSNQFKSEEERRQWEQFQAFQNQQNQQHQQYGQKKSKKGWFWGC
                     GGCLVLFILIIIGISACTAGVTGNLGGNSSKEANKTHKIGETVKNGDLEVTVNSVETM
                     KSVGPSLAPTNAKGIFVVADVTIKNKGKEALTIDSSMFKLKSGDKTFEADNTGSMSAN
                     QSDNGSIENSFFLQRINPDSTAQGKIVFDVSENIANAKDKKLEVISSLFSVKKITFDL
                     SDAKKTSKAKKDKQDTEVAVASSNSDNVNYEASATTPATTSSADTDSEDSEKSSKDED
                     KQNASKSDKSSVEKSESNEETATVEPMPQSKPTTSEAPPSQNNHNENNMYDASTE"
     gene            2020..2646
                     /locus_tag="PPY92_002392"
     CDS             2020..2646
                     /locus_tag="PPY92_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626366.1"
                     /translation="MKFKAIVAITLSFSLLTACGANQHKENNSKSNDTTKKTQETDNT
                     TQSNTEKQMTPQEAEDIVRNDYKARGANENQTLNYKTNLERSNEHEYYVEHLVRDAVG
                     TPLKRCAIVNRHNGTIINIFDDMSEKDKEEFEAFKKRSPKYNPGMSDHDKTDGESEDI
                     QHHDNDNNKAIQNDTPDQKAVDKNDKNAVNKEEKHDNHAKNSAETKVK"
     gene            2687..3028
                     /locus_tag="PPY92_002393"
     CDS             2687..3028
                     /locus_tag="PPY92_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3969 family protein"
                     /protein_id="HDJ2626367.1"
                     /translation="MITFENIQQLEKYTLMTMHGLFNQLKLGIISIDNAEHTLFTPYM
                     MESLSSLGVKPDIIDLIHKGTELEDFAAFNLSIEDTVTVCLQRTEELLNQYKNVEFND
                     KILINWRIIQE"
     gene            3129..3701
                     /locus_tag="PPY92_002394"
     CDS             3129..3701
                     /locus_tag="PPY92_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626368.1"
                     /translation="MEKNEYTAKYNEYSQLLDATYSQAVAYLLNKYGAVTDDYYKEKS
                     YTRFLNGEIKSISKGKYTRASEGLYCHHISEDKFQNLSDLRFISEFKYSYNVQKKENL
                     VYCDLIEHLILHAIITKESNGQFGVAGLCQMIKPTVIDWYISEYTPKPAWMQATKARA
                     YLPRILVEKLLIKIDDMLEGIEIYDFLESR"
BASE COUNT         1512 a          591 c          618 g         1027 t
ORIGIN      
        1 gttaaattat gttgtcatgt tcgtgtcatt ttgagtcgta atacaacttt acaattatta
       61 aattaacact tctacaatcc aaaaatattt ttaaagtttt gaaaaaaatt aaagtatttc
      121 cactactaat ttggtaaaat atgattatgt gatatagtga aagcgtttta cacttaataa
      181 ctccctctta aatgcatcca ggttttatgt agtaaatcat gaaaataaca tataaatata
      241 gaggagattt acctttgaat acagagaaca acaagaatca aaaccaatct gttaaaaatt
      301 ctgaaagacg cggcatgtta aaaggatgcg gcggttgcct tatttctttt attttattaa
      361 taatcttatt atcagcctgt tcaatgatgt ttagtaataa tgacaattcc actagtaatc
      421 aatcatcaaa aacgcaatta actcaaaaag atgaaaataa aaatgaagat aagcctgagg
      481 aaaaatcaga aacagctaca gatgaggatt tacaatcaac cgaagaagta cccgcaaatg
      541 aaaatactga aaataatcaa catgaaattg atgaaataac aacaaaagat caatcagatg
      601 atgaaattaa cacaccaaac gttgcagaag ataaatcaca agacgacttg aaagatgatt
      661 taaaagaaaa gcaacaatca agtaaccatc atcaatccac gcaacctaag acctcaccat
      721 caactgaaac aaacaggcaa caatcatttg ctaattgtaa gcaacttaga caagtttatc
      781 cgaatggtct cactgccgat catccagcat atcgtccaca tttagataga gataaagata
      841 atcgtgcatg tgaacctgat aaaaattaaa caacaagcga attgaattca aattgtattt
      901 agctttatgc actaatcaca tagtaaataa tgagggagat tttttaggca tgagcaatca
      961 attcaaaagt gaagaagaac gaagacaatg ggaacaattc caagctttcc aaaatcaaca
     1021 aaaccaacag caccagcaat acggacaaaa gaaatctaaa aaaggatggt tctggggttg
     1081 tggtggttgt ctagtattat ttattttaat tatcatcggt atttcagctt gtacagctgg
     1141 tgtcactggt aaccttggtg gaaatagttc taaagaagcg aacaaaaccc ataaaatcgg
     1201 tgaaactgtt aaaaatggcg accttgaagt cactgtaaat tcagtggaaa ctatgaaatc
     1261 tgtaggaccg tctcttgcac caacaaacgc taaaggtata tttgtcgttg ctgatgtgac
     1321 gattaaaaac aaaggtaaag aagcgttaac aattgatagt tcaatgttta agttaaaatc
     1381 cggtgataaa acatttgaag cagataatac aggttcaatg tctgctaatc aaagtgacaa
     1441 tggtagtata gaaaattcat ttttcttaca gcgtataaat cctgatagca ctgcacaagg
     1501 taaaattgtt ttcgatgtgt cagaaaacat agccaacgca aaagataaaa aattagaagt
     1561 tatttctagt ttatttagcg tcaagaagat tacatttgat ttatccgatg ctaaaaaaac
     1621 atcaaaagct aaaaaagaca agcaagatac agaagtagct gttgcgagtt caaatagcga
     1681 taatgtaaat tatgaagctt cggctactac acctgctaca acttctagtg cggatactga
     1741 ttctgaagat agcgaaaagt ctagtaaaga tgaggataaa cagaatgcgt ctaaaagtga
     1801 taaatctagt gtagaaaaaa gtgaatctaa tgaggaaact gctactgtag agcccatgcc
     1861 ccagagcaaa cctaccacta gtgaagcacc acctagtcaa aataatcaca acgaaaataa
     1921 catgtatgat gcttcaacag aataaatttt cagggtagct cagctaccct tctttttcgg
     1981 taaaaataaa tatacatagc aatataagga gattaaaaaa tgaaattcaa agctatcgtt
     2041 gcaatcacat tatcattttc actattaact gcctgcggtg ctaatcaaca taaagaaaat
     2101 aatagtaaat caaatgacac tactaaaaag acgcaagaaa ctgacaacac tacacagtca
     2161 aatacagaaa agcaaatgac accacaagaa gccgaagata tcgttcgaaa cgattacaaa
     2221 gcaagaggtg ctaacgaaaa tcaaacttta aattataaaa caaatcttga acggagtaat
     2281 gaacatgaat attatgttga acatctagtc cgcgatgcag ttggcacacc tttaaaacgt
     2341 tgcgctattg ttaatcgaca caatggtacg attattaata tttttgatga tatgtcagaa
     2401 aaagataaag aagaatttga agcatttaaa aagagaagcc ctaaatacaa cccaggtatg
     2461 agtgatcatg ataaaacaga tggtgagtca gaagacattc aacatcatga caatgataat
     2521 aacaaagcca ttcaaaatga tacaccagat caaaaagccg ttgataaaaa cgataaaaat
     2581 gctgttaata aagaagaaaa acatgataac catgcaaaaa attcagcaga aactaaagtt
     2641 aaataagggt atactttgat taattgtaat ttttaggaga aaaactatga taacatttga
     2701 gaatatacaa caacttgaaa aatacacctt aatgactatg catggtcttt ttaatcaact
     2761 taaactcggt attatttcaa tcgacaatgc agagcatacc ctctttacac cttatatgat
     2821 ggaatcactc tcttccctag gtgtgaaacc agacattatc gatttaattc acaaagggac
     2881 agagttagaa gactttgcag catttaattt atcaattgaa gatacagtta cagtctgttt
     2941 acaaagaact gaagaactat taaatcaata caaaaatgtg gaattcaatg acaaaatatt
     3001 aatcaattgg cgtattatac aagagtaata gacatataaa agtcgaatgt aactacgtga
     3061 gtactgattt tattctttgt aaattacaag catttcatat cataaagttt gaaaagaggt
     3121 atattgaagt ggagaaaaac gaatatacag ctaaatataa tgaatatagt caattattag
     3181 acgctactta ctcgcaagct gtagcatacc ttttaaataa atatggcgct gtaaccgatg
     3241 attattataa ggaaaaatca tacacgcgat ttttaaatgg agaaatcaaa agtatttcaa
     3301 aaggaaaata cactagagct agcgaaggat tatattgtca tcacataagc gaagacaaat
     3361 tccaaaactt atctgaccta agattcattt ccgaatttaa gtactcatac aacgttcaaa
     3421 agaaagaaaa cttagtgtac tgtgatctaa tcgagcattt aattttacat gcaattatta
     3481 caaaagaatc caatggccaa tttggtgtgg ctggattatg tcaaatgatc aaaccaacag
     3541 tcattgattg gtacattagc gaatatactc caaaaccagc atggatgcaa gccaccaaag
     3601 cacgtgccta tttgcctaga atattagtag agaaactact cattaaaatt gacgatatgt
     3661 tagaaggaat agaaatatat gatttccttg agtctagata aatgattaat ggagatttat
     3721 tttttgctgt tgagattttg ttatagat
//
LOCUS       DANBWH010000217         3682 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.225, whole genome shotgun sequence.
ACCESSION   DANBWH010000217 DANBWH010000000
VERSION     DANBWH010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.225"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            435..749
                     /locus_tag="PPY92_002395"
     CDS             435..749
                     /locus_tag="PPY92_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDJ2626369.1"
                     /translation="MTYKELATFLKVLSDSSRLEILDLLSCGELCACDLLAHFQFSQP
                     TLSYHLKALVKTNLVTTRKIGNKHLYQLNHNIFESVINNLSKVHTSNQRCFCHNLKTG
                     EC"
     gene            749..2041
                     /gene="arsB"
                     /locus_tag="PPY92_002396"
     CDS             749..2041
                     /gene="arsB"
                     /locus_tag="PPY92_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter membrane subunit
                     ArsB"
                     /protein_id="HDJ2626370.1"
                     /translation="MMTTLATLIFLVTLLFVLWQPKGLDIGITALTGAFIAVITGVVS
                     FSDVFEVTGIVWNATLTFVSVILISLILDKVGLFEWSAIHMLHASKGNGLKMFVYIIL
                     LGAIVAAFFANDGAALILTPIVLAMVKNIGFSKRAIFPFIIASGFIADTTSLPLIVSN
                     LVNIISADYFHVGFVRYFSRMIIPNLFSLLASIIVLWLYFRKAIPKTFDDNNIKHPKD
                     AINDLKLFKISWIVLVILLFGYLISEFTKIPVSIFTGIIAFIFLMLARKSNAVNIKQV
                     IKGAPWNIVLFSIGMYIVVFGLRNAGITLILAKILEYISNYGLFSTILGMGFISAFLS
                     SIMNNMPTVLIDAIAIGQSNVHGMLKEGLIYANVIGSDLGPKITPIGSLATLLWLHVL
                     TQKDVKISWGTYFKTGIIITIPVLFITLIGLYLTLIIF"
     gene            complement(2128..2982)
                     /locus_tag="PPY92_002397"
     CDS             complement(2128..2982)
                     /locus_tag="PPY92_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HDJ2626371.1"
                     /translation="MNKHKKGSIFGIIGLVVIFAVVSFLFFSMISDQIFFKHVKSDIK
                     IEKLNVTLNDAAKKQINNYTSQQVSNKKNDAWRDASATEIKSAMDSGTFIDNEKQKYQ
                     FLDLSKYQGIDKNRIKRMLVDRPTLLKHTDDFLKAAKDKHVNEVYLISHALLETGAVK
                     SELANGVEIDGKKYYNFYGVGALDKDPIKTGAEYAKKHGWDTPEKAISGGADFIHKHF
                     LSSTDQNTLYSMRWNPKNPGEHQYATDIKWAESNATIIADFYKNMKTEGKYFKYFVYK
                     DDSKHLNK"
     gene            complement(3258..3482)
                     /locus_tag="PPY92_002398"
     CDS             complement(3258..3482)
                     /locus_tag="PPY92_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626372.1"
                     /translation="MDYAHLNLEHFFARNDDLDVIRDRADFVMINNFTNEMMYRDGQI
                     EGTIDLNQYYYKNRSNAASFIMMDYKKETK"
BASE COUNT         1174 a          590 c          502 g         1416 t
ORIGIN      
        1 aaggtaagct attatttcac tttcacaacc attaaaccat ttaaaacttt tcatttaata
       61 tcaaccttag tgtttgtgtt aaaatatttt taacgaggtg aattaaattg aaatttatag
      121 caaagatatt aatctcatta gtaattctat ttatgatttt tagtcgtaaa aacaaagctt
      181 cttaatcaat acccttgatt acaaaataaa ttcaactaag gtgattggcc atacactcaa
      241 gatatggcta atcacctttt tcaatgcaaa acaaagagta tattttgcaa aatcaatatc
      301 tataatttat ctttaatacc cttttaatac taaaaccaaa ttcaaattac atagtcatat
      361 cgtgtaatat accaaaggaa tatagacatt catctatata gatgataatc tatttaacgt
      421 ctaggaggta tgttatgacg tataaagaac tagcaacatt tttaaaagtt ttatcagatt
      481 caagcagatt agaaatacta gatttacttt cttgtggaga gttatgcgct tgtgatttgt
      541 tagcacattt tcaattctct caacccacac ttagctatca tttgaaagca ttagtaaaaa
      601 ccaacttagt tacgacacga aaaatcggaa ataaacattt ataccagctt aatcataata
      661 tttttgagtc cgtaattaat aacttgtcta aggttcatac ctctaaccaa cgatgttttt
      721 gtcataacct taagactggt gaatgctaat gatgacaact ttagcgacac tcatttttct
      781 agtaacttta ttatttgtat tatggcaacc taaaggccta gatattggca ttaccgcatt
      841 aactggtgcc tttattgctg ttattactgg tgttgtaagt ttttccgatg ttttcgaagt
      901 aacaggtatt gtttggaatg ctactttgac ttttgtctca gtcattctta tttcattaat
      961 attagataaa gttggattat tcgaatggtc agctattcac atgcttcatg cttcaaaagg
     1021 aaatggttta aaaatgttcg tttatatcat attattgggt gccattgttg ctgcattttt
     1081 cgcaaatgat ggcgcagcgt taatcttaac gcctattgta ttagcgatgg ttaaaaatat
     1141 aggttttagt aagcgggcca tattcccttt tattattgcg agtggtttta tagctgacac
     1201 aacttcttta cctttgatcg tgagcaatct agtgaatatt atatctgctg attattttca
     1261 tgtaggattc gttcgatatt ttagtagaat gattatacct aatttattct cgcttttagc
     1321 aagtattata gtattgtggt tatattttag aaaggcgata cctaaaacgt ttgatgataa
     1381 taatataaag catcctaaag atgccattaa tgatttaaag ctatttaaaa tttcatggat
     1441 tgttctagtt atattacttt tcggctatct aatcagtgaa tttactaaaa ttccggtatc
     1501 aattttcact ggaatcattg cttttatttt tctaatgttg gctcgtaaat caaatgctgt
     1561 aaatattaag caagtcatta agggcgcacc ttggaatata gtattatttt caattggtat
     1621 gtatatcgtc gtattcggct taagaaatgc tggcattact ttaatattgg ctaaaatatt
     1681 agaatatatt tccaattacg gtctatttag caccattttg ggaatgggct tcatttcagc
     1741 gtttttatca tcaataatga ataatatgcc tacagtttta atagatgcga ttgctattgg
     1801 tcaatcaaat gtccatggca tgttaaaaga aggcctaatt tatgcgaatg ttatcggttc
     1861 tgatttaggt ccaaaaatta caccgatagg ctctttagct acattactgt ggttacacgt
     1921 cttaacacaa aaagatgtta agatttcttg gggcacatac tttaaaactg gtatcatcat
     1981 tacaattcca gtactattta taaccctcat agggttgtat ctaacactta tcatatttta
     2041 agaaataaaa agacgctttc tatttattgc gatagaaagc gtctttttat acttgagcaa
     2101 gttatatcat aacttaactc tatcaaatta cttattcaaa tgtttactgt catctttata
     2161 cacaaagtat ttgaagtatt ttccttcagt cttcatattc ttataaaagt cagcgataat
     2221 tgttgcatta ctttctgccc acttaatatc agtagcatat tgatgttctc ctggattttt
     2281 tggattccat ctcatactat acaatgtatt ttgatctgtg cttgataaga agtgcttatg
     2341 aatgaaatca gcaccgcctg aaatagcttt ttcaggtgta tcccaaccat gctttttagc
     2401 atattctgca cctgttttaa ttgggtcttt atcaagggct cctactccat agaaattgta
     2461 gtattttttg ccatcaattt cgactccatt agctaattca cttttaactg cgccagtttc
     2521 taataatgca tgtgaaatta aataaacttc gttaacgtgc ttatctttag cagcttttaa
     2581 gaaatcatcc gtatgtttca ataacgttgg tctatctact aacatacgtt taattctatt
     2641 tttatcaatc ccttgatact ttgataaatc taaaaattga tatttttgct tttcattatc
     2701 gataaaagta ccgctatcca ttgcactttt aatttcagtt gcagatgcat ctctccatgc
     2761 atcattcttt ttatttgata cctgttgact cgtataatta tttatttgtt tctttgctgc
     2821 atcgtttaat gtaacattta acttttcaat cttaatgtcg gatttaacat gtttgaaaaa
     2881 tatctgatcg gatatcattg agaaaaataa aaatgagaca acagcaaata tgacaacaag
     2941 tcctattatt ccaaaaatag aacctttctt gtgtttattc atatccacac ctcttaggtc
     3001 attgttgtta tacattcagt ttgactacga taataaagtt taacattatt aaaaccgttt
     3061 aacaatttta tctatgcatt gttacatatt cataaataaa cagtaatgta aatgaaacgt
     3121 tttaaaccat ttatcaacat tactttacgt tgatctacga atcataaatg ttttatcttt
     3181 ttgtataacc tttgaaaacc tttatatcaa taatcttatt attcacgtat taaaaaacaa
     3241 aaaattcttt tgttcgttta cttagtttct tttttataat ccatcataat aaaacttgct
     3301 gcatttgatc tatttttata atagtactga tttaaatcaa tcgtgccttc aatttgacca
     3361 tcacgataca tcatttcatt agtgaagtta tttatcatca cgaaatcagc gcgatctctt
     3421 ataacatcta aatcgtcgtt tcgtgcaaaa aaatgttcta aatttaaatg tgcgtaatcc
     3481 attgttacct cccgattaca aattttgttc tatctactat ttagcaaagt aataatacat
     3541 catatcacat taaattaatt acttatgtct tatatttaac acttaattta gcgattacgc
     3601 aaatgaatga tttttctaaa tattttattc taactttatc cggaaatatt tgttatacgt
     3661 ataacttgga gaatgactac tt
//
LOCUS       DANBWH010000218         3681 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.226, whole genome shotgun sequence.
ACCESSION   DANBWH010000218 DANBWH010000000
VERSION     DANBWH010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.226"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(459..938)
                     /gene="rlmH"
                     /locus_tag="PPY92_002399"
     CDS             complement(459..938)
                     /gene="rlmH"
                     /locus_tag="PPY92_002399"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020363758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDJ2626373.1"
                     /translation="MKITILAVGKLKEKYWKQAIAEYEKRLGPYTKIDIIEVPDEKAP
                     ENMSDKEIEQVKEKEGQRILAKIKPQSTVITLEIQGKMLSSEGLAQELNQRMTQGQSD
                     FVFVIGGSNGLHKDVLQRSNYALSFSKMTFPHQMMRVVLIEQVYRAFKIMRGEAYHK"
     gene            complement(1306..3624)
                     /gene="adsA"
                     /locus_tag="PPY92_002400"
     CDS             complement(1306..3624)
                     /gene="adsA"
                     /locus_tag="PPY92_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored adenosine synthase AdsA"
                     /protein_id="HDJ2626374.1"
                     /translation="MKALLLKTSVWLVLLFSVMGLWQVSNAAEQYTPIKAHVVTTIDK
                     ATTDKQQVTPTKEAAHQFGEEAATNVSASAQGTADEINNKVTSNAFSNKPSTAVSTKV
                     NETHDVDTQQASTQKPTQSATFTLSNAKTASLSPRMFAANVPQTTTHKILHTNDIHGR
                     LAEEKGRVIGMAKLKTIKEQEKPDLMLDAGDAFQGLPLSNQSKGEEMAKAMNAVGYDA
                     MAVGNHEFDFGYDQLKKLEGMLDFPMLSTNVYKDGKRAFKPSTIVTKNGIRYGIIGVT
                     TPETKTKTRPEGIKGVEFRDPLQSVTAEMMRIYKDVDTFVVISHLGIDPSTQETWRGD
                     YLVKQLSQNPQLKKRITVIDGHSHTVLQNGQIYNNDALAQTGTALANIGKVTFNYRNG
                     EVSNIKPSLINVKDVENVTPNKALAEQINQADQTFRAQTAEVIIPNNTIDFKGERDDV
                     RTRETNLGNAIADAMEAYGVKNFSKKTDFAVTNGGGIRASIAKGKVTRYDLISVLPFG
                     NTIAQIDVKGSDVWTAFEHSLGAPTTQKDGKTVLTANGGLLHISDSIRVYYDMNKPSG
                     KRINAIQILNKETGKFENIDLKRVYHVTMNDFTASGGDGYSMFGGPREEGISLDQVLA
                     SYLKTANIAKYDTTEPQRMLLGKPAVSEQPAKGQQGSKGSESGKDVQPIGDDKAMNPA
                     KQPATGKVVLLPTHRGTVSSGTEGSGRTLEGATVSSKSGNQLVRMSVPKGSAHEKQLP
                     KTGTNQSSSPAAMFVLVAGIGLIATVRRRKAS"
BASE COUNT          936 a          731 c          589 g         1425 t
ORIGIN      
        1 tgtgcttata taaaagcgtc acattattaa aacttattat ttatcataaa aactactttg
       61 ctacgttcaa gtttgtcttt attaaaaata aaatccgaat catttattat acttaagttt
      121 caaataggtg ttaataaatc attataaatt tctagacact agtcttagca aagtgaaatt
      181 caaaatttta ctaaatgaaa aatatgtcta tatatgtact ttaaaaaatt tcattagccg
      241 cttatctagt atcgtaatca ttcctcacat atttattagc atcttcttta catcgcttac
      301 tgcaaacatc taatacaaaa agaagtcgat ttacacacca tgtattaaat aatggaaatt
      361 cttaatcttt acttgtacct aaattatcaa acttaatatt cactttttat tcttcaaaga
      421 tttgagctaa tttaataatt ttctcatatt ttttagtttt atttgtggta cgcttctcca
      481 cgcataatct taaatgctct atacacttgc tcaattaaca caacccgcat catttgatgt
      541 gggaatgtca ttttgctgaa tgatagtgcg tagttactgc gttgtaagac gtccttgtgc
      601 aggccgtttg atccgccaat gacgaataca aagtcgcttt gcccttgggt catgcgttgg
      661 ttcaattctt gggccaatcc ttcggaagat agcatctttc cttgtatttc taatgtaatg
      721 actgtggatt gtggtttaat tttggctagt attcgttggc cttctttttc ttttacttgc
      781 tcaatttctt tgtcgctcat attttctggt gctttttcgt ctggaacttc tatgatgtct
      841 atcttggtgt atgggcctaa acgtttttca tattctgcta tggcttgctt ccaatatttc
      901 tcttttagtt tccctacagc taaaatggtg attttcatgt cgtttggtcc tccaaattgt
      961 tatcaacttt ccagttatcc acaagttatt aacttgttca cactgttccc tcttattata
     1021 ccaatatttt ttgcagtttt tgatattttc ctgacattta tgcccaattt ttagtgtata
     1081 acttgtgtat ttgtggataa ctctgtgggc attgtgggta tttctcttta aattgtacac
     1141 aattctggat aactatcccc atttctgtgg ataaacacct tgatgtctta ttacttatcc
     1201 ccgtttttat atcccatgtt tatcaacgtt tatagctttt ttccataaaa aaacactacc
     1261 gtacctctta tttaagaaat acagtagtat tgttttcaat atattttaac tagcttttct
     1321 acgtcgtaca gtcgcgatta aacctatacc tgctactaat acaaacatcg ctgctgggct
     1381 tgagctttga ttagttccag tttttggtaa ctgtttctca tgcgcgctac ctttaggcac
     1441 tgacattcta accaattggt tcccactctt gcttgataca gtagctcctt ctaatgtgcg
     1501 accagaacct tctgtaccgc tactaacagt tcctctatgc gttggtaaca atacaacttt
     1561 acctgtcgct ggttgtttcg ctggattcat cgctttgtcg tcaccaattg gttgtacatc
     1621 tttaccagac tcactacctt tgctaccttg ttgtccttta gctggttgtt cacttactgc
     1681 tggtttacct aataacatac gttgtggttc tgtcgtatca tacttagcta tgttagctgt
     1741 ttttaaataa cttgctagta cttgatctaa tgaaatacct tcttctctag ggccaccgaa
     1801 catactatat ccgtcgccac ctgatgctgt gaagtcattc atcgttacat gatatacacg
     1861 ttttaaatca atattttcaa acttacctgt ctctttattt aaaatttgaa tagcgttaat
     1921 tcgtttgcca gacggtttat tcatatcata gtaaacacga attgaatcag agatatgtag
     1981 taaaccgcca ttcgctgtta atactgtctt accgtctttt tgtgttgttg gtgcacctaa
     2041 actatgttcg aaagctgtcc agacgtctga accttttaca tcaatttgcg caatcgtatt
     2101 tccaaatggt aatactgaga ttaaatcata gcgtgtcacc ttaccttttg cgatagaggc
     2161 acgaatacct ccaccatttg tcacggcaaa gtcagtcttt ttagagaaat tcttaacgcc
     2221 atacgcttcc atagcatctg caatcgcgtt tcctaaattt gtttcacgcg ttctaacgtc
     2281 atctctttct cctttgaaat caatggtatt atttggaata ataacctctg ctgtttgtgc
     2341 tctaaatgtt tgatcagctt gattaatttg ttcagctaat gctttgttcg gtgttacatt
     2401 ttcaacgtct ttaacattaa tcaatgacgg tttaatattt gatacctctc cattgcggta
     2461 attaaatgta accttaccga tattcgcaag tgctgtacct gtttgtgcta atgcatcatt
     2521 gttataaatt tgaccatttt gaagtacggt atgtgaatga ccatcaatga ctgtaatacg
     2581 tttcttcaat tgtggatttt gacttaattg tttcactaag taatcaccac gccatgtttc
     2641 ttgtgttgaa ggatcaatcc ctaaatgtga tataacaaca aatgtatcta cgtctttata
     2701 aatacgcatc atttctgctg tcacactttg taatggatct ctaaattcaa cacctttaat
     2761 gccctcaggt cttgttttcg tctttgtttc tggtgtcgtt acgccaataa ttccataacg
     2821 aataccattt ttcgttacaa ttgttgaagg cttaaacgcg cgtttcccat ctttgtaaac
     2881 gttagtactt agcatcggga agtctaacat accctctaac tttttcaact gatcgtatcc
     2941 aaagtcaaat tcatggttac ccactgccat agcatcataa cctactgcat tcattgcttt
     3001 agccatttct tcacctttag actggtttga aagtggtaaa ccttggaagg cgtctcctgc
     3061 gtctaacatt aaatcaggct tttcttgttc ttttattgtt tttaatttag ccataccgat
     3121 gacacgccct ttttcttcgg ctagtcggcc atggatatca tttgtatgta atattttatg
     3181 tgttgttgtt tgtggtacat tggcagcaaa cattcgtggt gaaagtgatg ctgttttagc
     3241 atttgataat gtgaatgttg ctgattgagt tggtttttgt gttgaggctt gttgtgtatc
     3301 tacatcgtgc gtttcgttta cttttgttga aactgctgta gatggtttgt tagaaaatgc
     3361 gttggatgtt actttattgt ttatttcatc agctgttccc tgtgctgatg ctgatacgtt
     3421 ggttgccgct tcttcaccaa attgatgagc cgcttccttt gttggcgtta cttgttgctt
     3481 atctgttgtt gctttgtcta tcgttgttac tacatgtgct ttgattggtg tatactgctc
     3541 agccgcgttc gagacttgcc ataatcccat cacactaaaa agcaaaacga gccatacact
     3601 tgttttaagt aataaagctt tcatttcgtt ccctccatta actgtcatac accaatatat
     3661 tatcataatc cttatattaa a
//
LOCUS       DANBWH010000219         3673 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.227, whole genome shotgun sequence.
ACCESSION   DANBWH010000219 DANBWH010000000
VERSION     DANBWH010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.227"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(448..3564)
                     /gene="fnbA"
                     /locus_tag="PPY92_002401"
     CDS             complement(448..3564)
                     /gene="fnbA"
                     /locus_tag="PPY92_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbA"
                     /protein_id="HDJ2626375.1"
                     /translation="MKNNLRYGIRKHKLGAASVFLGTMIVVGMGQDKEAAASEQKTTT
                     VEENGNSATDNKTSETQTTATNVNHIEETQSYNATVTEQPSNATQVTTEEAPKAVQAP
                     QTAQPANVETVKEEEKPQVKETTQPQDNSGNQRQVDLTPKKVTQNQGTETQVEVAQPR
                     TASESKPRVTRSADVAEAKEASDVSEVKGTDVTSKVTVESGSIEAPQGNKVEPHAGQR
                     VVLKYKLKFADGLKRGDYFDFTLSNNVNTYGVSTARKVPEIKNGSVVMATGEILGNGN
                     IRYTFTNEIEHKVEVTANLEINLFIDPKTVQSNGEQKITSKLNGEETEKTIPVVYNPG
                     VSNSYTNVNGSIETFNKESNKFTHIAYIKPMNGNQSNTVSVTGTLTEGSNLAGGQPTV
                     KVYEYLGKKDELPQSVYANTSDTNKFKDVTKEMNGKLSVQDNGSYSLNLDKLDKTYVI
                     HYTGEYLQGSDQVNFRTELYGYPERAYKSYYVYGGYRLTWDNGLVLYSNKADGNGKNG
                     QIIQDNDFEYKEDTAKGTMSGQYDAKQIIETEENQDNTPLDIDYHTAIDGEGGYVDGY
                     IETIEETDSSAIDIDYHTAVDSEVGHVGGYTESSEESNPIDFEESTHENSKHHADVVE
                     YEEDTNPGGGQVTTESNLVEFDEESTKGIVTGAVSDHTTIEDTKEYTTESNLIELVDE
                     LPEEHGQAQGPIEEITENNHHISHSGLGTENGHGNYGVIEEIEENSHVDIKSELGYEG
                     GQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNKGDQSFEEDTEKDKPKY
                     EHGGNIIDIDFDSVPQIHGFNKHNEIIEEDTNKDKPNYQFGGHNSVDFEEDTLPKVSG
                     QNEGQQTIEEDTTPPTPPTPEVPSEPETPMPPTPEVPSEPETPTPPTPEVPSEPETPT
                     PPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEVPAEPGKPVPPAKEEPKKPSKPVEQ
                     GKVVTPVIEINEKVKAVAPTKKAQSKKSELPETGGEESTNKGMLFGGLFSILGLALLR
                     RNKKNNKA"
     gene            complement(3548..3650)
                     /locus_tag="PPY92_002402"
                     /pseudo
     CDS             complement(3548..3650)
                     /locus_tag="PPY92_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501263.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          892 a          725 c          579 g         1477 t
ORIGIN      
        1 aaattaaatt caaattcatc taatttaaag caatatacta aagcatacat aataatcgcc
       61 tgtacaatgc atcattaaca agtcactgaa atgcctttca ttgtattaat aacgtcatta
      121 taattttata tcgttcggtt tttgttttat tttaatgatt atttatacag aaacagccgt
      181 atttcaagac gacattttaa gtttaactaa atttgcatct agttaataat tgcatttatc
      241 aaatttgtct tattgattca atctaatttg tactaacaaa ctagttttaa attctaactt
      301 tatctctcat tttgtaatca atcatcagac ataaagcgct tcagcaatca gaaaacactc
      361 taattttcta ttagaaattt gatttaatct aaaaaaacag gtttacttca tataatttat
      421 gaaataaacc cgtcaatttt tgtttaatta tgctttgtta ttctttttat ttctgcgtaa
      481 taatgctaaa cctagaatgc tgaataatcc gccgaacaac atacctttgt ttgttgattc
      541 ttctccacct gtttcaggta gttcagattt cttagattgt gcttttttag ttggtgccac
      601 tgctttaacc ttttcattga tttcaataac aggtgttact actttacctt gttccactgg
      661 tttagaaggc tttttaggtt cttcttttgc gggtggtact ggtttaccag gttcagctgg
      721 tacctctggt gttggcggtg ttggtgtttc tggctcactc ggcacttctg gtgtcggtgg
      781 tgttggtgtt tccggctcac ttggtacttc cggagtcggt ggtgttggtg tttccggctc
      841 acttggtacc tctggtgttg gtggcgttgg tgtttccggc tcactcggta cttctggtgt
      901 cggtggcatt ggtgtttccg gctcactcgg tacttctggt gtcggtggcg ttggcggcgt
      961 tgtatcttct tcaatcgttt gttgaccttc attttggccg cttacttttg gaagtgtatc
     1021 ttcttcaaag tcaacactat tgtgtccacc gaattgataa ttaggtttat ctttgtttgt
     1081 atcttcttca ataatttcat tatgcttatt gaatccatga atttgtggca cactgtcgaa
     1141 gtcgatatca atgatattac cgccatgttc atatttaggc ttgtctttct ctgtatcttc
     1201 ttcgaatgac tggtcacctt tattttgacc atgaatttga ggtacactgt cgaaatcgat
     1261 atctacgata ttgccacctt gttcatattt aggtttgtct tcttctgtgt cttcctcgaa
     1321 tgactggtta ccgctatttt ggccaccttc gtaacctaat tcactcttaa tatcaacgtg
     1381 gctattttct tcgatttctt caatcacgcc ataattaccg tgaccatttt cagttcctaa
     1441 accagaatga gaaatatgat gattgttttc agtaatttcc tcgattggtc cttgtgcttg
     1501 accatgttct tcaggtagtt catctactag ttcaatcaga ttactttcag tcgtatattc
     1561 tttcgtatct tcaattgttg tatggtcgct cactgcgcca gttacaatac cttttgtaga
     1621 ctcttcgtca aattcaacta agttagactc agttgttact tggccaccac ctggatttgt
     1681 atcctcttca tattcaacaa catcagcgtg atgttttgaa ttttcatgtg tcgattcttc
     1741 aaagtcaatt ggatttgatt cctcagagga ctcagtgtat cctccaacgt gacccacttc
     1801 actatccaca gcagtatggt aatcgatatc aatagctgat gaatccgttt cttctattgt
     1861 ttcaatatac ccatcaacat aaccaccctc accatctata gctgtgtggt aatcaatgtc
     1921 aagcggtgta ttgtcttgat tttcttctgt ttcaataatt tgcttggcat cgtactgccc
     1981 gctcatagtt ccttttgcag tatcttcttt atattcaaaa tcattatctt gaataatttg
     2041 tccattttta ccattgccgt cagctttatt gctatataaa actaaaccat tatcccaagt
     2101 taaacgatat cccccataaa catagtaaga tttatatgct cgttctggat acccatataa
     2161 ttcagttcta aaattaacct gatctgaccc ttgcaaatat tcacctgtat aatgaatgac
     2221 atacgtttta tccaacttat ctaaattcaa tgagtaacta ccattgtctt gcacactcaa
     2281 ttttccattc atttcctttg ttacatcttt gaatttgtta gtatctgatg tatttgcata
     2341 aacactttgt ggcaattcat cttttttccc tagatattca tatactttaa cagtaggttg
     2401 tccaccagct aaattactac cttcagtcaa cgtccctgtt actgatacag tgtttgactg
     2461 gtttccattc attggcttaa tataagctat atgtgtaaat ttattagatt ctttattaaa
     2521 tgtttcaatt gatccattta catttgtata actattgcta acacctggat tataaacaac
     2581 tggtattgtt ttttctgttt cttcaccatt taatttagaa gtaatctttt gttctccatt
     2641 gctttgtaca gttttagggt caataaataa gttgatttct aaattagctg ttacctctac
     2701 cttgtgttca atttcgttag taaatgtata tcttatgtta ccattcccta agatctcacc
     2761 tgtagccatt acaactgagc catttttaat ctctggtacc tttctagctg ttgaaacccc
     2821 ataagtattt acattatttg ataatgtaaa atcaaaataa tctcctcttt ttaatccatc
     2881 tgcgaatttc aatttgtatt tcaatacgac acgttgacca gcatgtggct ctactttatt
     2941 tccttgaggt gcctcaatag aaccactttc tactgtaact ttacttgtaa catctgtgcc
     3001 tttaacttct gaaacgtcac tagcttcctt agcttccgct acatctgctg atcttgtcac
     3061 acgtggctta ctttctgatg ccgttcttgg ctgtgccact tcaacttgtg tttctgtccc
     3121 ttgattttgt gtaacctttt taggtgttaa atctacttgt ctttgatttc cgctattgtc
     3181 ttgaggttgt gtcgtttcct taacttgagg tttctcttct tctttaactg tttctacatt
     3241 tgctggttgt gcagtttgtg gtgcttgtac tgcttttggt gcttcttcag ttgttacttg
     3301 tgttgcgttt gacggttgtt ctgttactgt tgcgttatat gattgagttt cttctatatg
     3361 attaacgtta gtagcagttg tttgtgtttc acttgtttta ttatcagtag ctgaattccc
     3421 attttcttct actgtagttg tcttttgttc tgatgctgca gcttctttat cttgtcccat
     3481 cccaacaacg atcattgttc ctaagaatac tgatgctgct cccaatttat gttttctaat
     3541 gccgtaccta agattgtttt tcactataat atctcccttt aaatgcaaaa ttcattaatt
     3601 tttttaaact taataaatgc aagtctatat tgttcggttt ttaaaagcaa tacaaaaaag
     3661 cgagttaatg aaa
//
LOCUS       DANBWH010000220         3655 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.228, whole genome shotgun sequence.
ACCESSION   DANBWH010000220 DANBWH010000000
VERSION     DANBWH010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.228"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            146..439
                     /locus_tag="PPY92_002403"
                     /pseudo
     CDS             146..439
                     /locus_tag="PPY92_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(575..1477)
                     /gene="cvfB"
                     /locus_tag="PPY92_002404"
     CDS             complement(575..1477)
                     /gene="cvfB"
                     /locus_tag="PPY92_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HDJ2626376.1"
                     /translation="MALDKDIVGSIEFLEVVGLQGSTYLLKGPNGENVKLNQSEMNDD
                     DELEVGEEYSFFIYPNRSGELFATQNMPDITKDKYDFAKVLKTDRDGARIDVGLPREV
                     LVPWEDLPKVKSLWPQPGDHLLVTLRIDRENHMYGRLASESVVENMFTPVHDDNLKNE
                     VIEAKPYRVLRIGSFLLSESGYKIFVHESERKAEPRLGESVQVRIIGHNDKGELNGSF
                     LPLAHERLDDDGQVIFDLLVEYDGELPFWDKSSPEAIKEVFNMSKGSFKRAIGHLYKQ
                     KIINIETGKITLTKKGWSRIDSKE"
     gene            1624..3225
                     /locus_tag="PPY92_002405"
     CDS             1624..3225
                     /locus_tag="PPY92_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDJ2626377.1"
                     /translation="MLQVTDVSLRFGDRKLFEDVNIKFTEGNCYGLIGANGAGKSTFL
                     KILSGELDSQTGHVSLGKNERLAVLKQDHYAYEDERVLDVVIKGHERLYEVMKEKDEI
                     YMKPDFSDEDGIRAAELEGEFAEMNGWNAEADAANLLSGLGIDPTLHDKKMAELENNQ
                     KIKVLLAQSLFGEPDVLLLDEPTNGLDIPAISWLEDFLINFDNTVIVVSHDRHFLNNV
                     CTHIADLDFGKIKVYVGNYDFWYQSSQLAQKMAQEQNKKKEEKMKELQDFIARFSANA
                     SKSKQATSRKKQLEKIELDDIQPSSRRYPFVKFTPEREIGNDLLIVQNLSKTIDGEKV
                     LDNVSFTMNPNDKAILIGDSEIAKTTLLKILAGEMEPDEGSFKWGVTTSLSYFPKDNS
                     EFFEGVNMNLVDWLRQYAPEDEQTETFLRGFLGRMLFSGEEVKKKASVLSGGEKVRCM
                     LSKMMLSSANVLLLDEPTNHLDLESITAVNDGLKSFKGSIIFTSYDFEFINTIANRVI
                     DLNKQGGVSKEIPYEEYLQEIGVLK"
BASE COUNT         1209 a          613 c          628 g         1205 t
ORIGIN      
        1 ggtgccgtct cagccacccc aaccggcaca ttgttgtaat ctgactatat gtcagcttct
       61 gtgttggggc ccctgtcttc gactggcact gctccctcag gagtctcgcc attaatacta
      121 cgtattaaca tgtaatttta ctttgaaata cattaaagcc caaattaatc aaaagctttc
      181 tgaaccagaa acgaaaaaaa tctatagtca tagaaaaatt tatgtagagc ctgtttttgg
      241 atttatgaag gttattttgg gtttcactcg aatgtcagtt cgaggaataa ataaagttaa
      301 acgagagcta ggttttgtat taatggcact taatataagg aaaatagcag ctcaacgagc
      361 tgtacattat aaaatacata tcaaaaaagc taatttccat caaataatta atagaaatca
      421 gcttttttac attgcctaag aacttaatgt cccagcctat tcgtttgaat tatttaattt
      481 tttactttga aaggaatgtt taaaaaacta cttcatctgt ccgacttaat attgaatata
      541 aaaaacacgc atcctacgac acgtgtaaaa atgattattc ttttgagtca attcgactcc
      601 aacctttttt agttaaagtg attttacctg tttctatatt aataatcttc tgtttatata
      661 agtgaccgat tgcacgtttg aatgaacctt tactcatatt gaatacttct ttaatcgctt
      721 cagggcttga tttgtcccag aatggtaatt caccatcata ttcaactagt aaatcaaaga
      781 tgacttggcc gtcatcgtct aaacgttcat gtgcaagtgg taaaaatgaa ccatttaact
      841 cacctttatc attatgcccg ataattctaa cttgaacaga ttcacctaat cttggttcag
      901 ctttacgttc tgattcatgt acgaaaattt tgtaacctga ttcgcttaat aagaagctac
      961 caattcgtaa tacgcggtaa ggtttggctt caatgacttc gttttttaaa ttatcatcgt
     1021 gtacaggtgt aaacatattt tctacaacag attcactcgc taaacgtcca tacatatgat
     1081 tctcacggtc aattcgtaat gtaactagca aatgatcacc aggttgtggc catagtgatt
     1141 tcacttttgg taaatcttcc catggtacta acacttcacg gggtaaacca acatctatac
     1201 gtgccccatc gcgatccgtt ttaagtactt tagcaaaatc atatttatct ttcgtaatat
     1261 caggcatatt ttgagttgca aataattcac ctgaacggtt tggataaatg aagaaactat
     1321 attcttcacc tacttctaat tcatcatcat cgttcatttc tgattggttt aactttacgt
     1381 tttcaccgtt tggtcctttt aaaaggtaag ttgagccttg taaccctact acttcaagga
     1441 attctataga acctactata tctttgtcta atgccatgtt attctccttc gattcttgta
     1501 ttctttatta ttataacatg tgtgagtcat tctatcatta caacttatat gaggtcgaaa
     1561 ctgtgacgat aatatgatat aattcttccg ttagaatatc gaatgaaaaa aaggagaaaa
     1621 tgcatgttac aagtaactga tgtgagttta cgttttggag atcgtaaact atttgaagat
     1681 gtaaatatta aatttacaga aggtaattgt tatggattaa ttggtgcgaa tggtgcaggt
     1741 aaatcaacat tcttaaaaat attatctggt gaattagatt ctcaaacagg acatgtttca
     1801 ttaggtaaaa atgaacgtct agctgtttta aaacaggacc actatgctta tgaagatgaa
     1861 cgcgtgcttg atgttgtaat taaaggtcac gaacgtcttt atgaggttat gaaagaaaaa
     1921 gatgaaatct atatgaagcc agatttcagt gatgaagatg gtatccgtgc tgctgaactt
     1981 gaaggtgaat ttgcagaaat gaatggttgg aatgctgaag ctgatgctgc taacctttta
     2041 tctggtttag gtatcgatcc aactttacac gataaaaaaa tggctgaatt agaaaacaac
     2101 caaaaaatta aagtattatt agcgcaaagt ttattcggtg aaccagacgt actattactg
     2161 gatgagccta ctaacggtct cgatattcca gcaatcagtt ggttagaaga tttcttaatt
     2221 aactttgata atactgttat cgtagtatcg catgaccgtc atttcttaaa taatgtatgt
     2281 actcatatcg ctgatttaga cttcggtaaa attaaagttt atgttggtaa ctatgatttt
     2341 tggtatcaat ctagtcagtt agctcaaaag atggctcaag aacaaaacaa gaaaaaagaa
     2401 gaaaaaatga aagagttaca ggactttatt gcacgtttct cagctaacgc ttctaaatct
     2461 aaacaagcaa caagtcgtaa aaaacaactt gagaaaattg aattagatga tattcaacca
     2521 tcatcaagaa gatatccttt cgttaaattc acgcctgagc gtgagattgg taacgactta
     2581 ttaatcgttc aaaatctttc taaaacaatt gacggcgaaa aagtattaga taatgtatca
     2641 ttcacaatga atccaaatga taaagcgatt ttaattggag atagtgaaat tgcaaaaaca
     2701 acattactta aaatattagc tggcgaaatg gaaccagacg aaggttcatt taaatggggt
     2761 gttactacat cattaagtta cttccctaaa gataactcag agttctttga gggtgtaaat
     2821 atgaatctcg ttgattggtt aagacaatat gctcctgaag atgaacaaac agaaacattt
     2881 ttacgtggtt tcttaggtcg tatgttattt agtggtgaag aagttaagaa aaaagctagt
     2941 gtgctttcag gtggagaaaa agtacgttgt atgctaagta aaatgatgtt atcaagtgcg
     3001 aatgtacttt tacttgacga acctactaac cacttagact tagaaagtat tactgctgtc
     3061 aatgatggtc ttaaatcatt taaaggttct atcatcttta cttcttatga cttcgaattt
     3121 atcaacacga ttgcaaaccg tgttatcgat ttaaataaac aaggcggcgt ttcaaaagaa
     3181 attccatatg aagaatactt gcaagaaatc ggcgttttaa aataaacata atatgtagaa
     3241 tttcttttcg aaaatctctg tattgaggcc cgaccaactt gcacattatt gtaagttggc
     3301 ttactgtcac cttctatatt gggacttatt ccgcaacttg tattgtttgt agaaattagg
     3361 aatccaattt ctctatgtgt tgcaccgaca aggattgaaa aaagctagct acaaagtcat
     3421 tttcattcag tcaactactg tcaatataac attgcagggt ctagggcatt gattcatgcc
     3481 cgagactata ttcaatttaa tagacattaa ctggtcgtga ggctgggaca taaatcccta
     3541 aaaaaacagc agtaagataa ttttcaatta gaaaatatct tactgctgtt ctctatttat
     3601 acaatacttc gtattgaatg gcttcgcttt gcccgtctgg cacattactg taaaa
//
LOCUS       DANBWH010000221         3652 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.229, whole genome shotgun sequence.
ACCESSION   DANBWH010000221 DANBWH010000000
VERSION     DANBWH010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.229"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            156..611
                     /locus_tag="PPY92_002406"
     CDS             156..611
                     /locus_tag="PPY92_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDJ2626378.1"
                     /translation="MVKHLQDHIQFLEQFINNVNALTAKMLKDLQNEYEISLEQSNVL
                     GMLNKEPLTISEITQRQGVNKAAVSRRIKKLIDAKLVKLDKPNLNIDQRLKFITLTDK
                     GRAYLKERNAIMTDIAQDITNDLNSEDIENVRQVLEVINHRIKTYSNHK"
     gene            851..2233
                     /locus_tag="PPY92_002407"
     CDS             851..2233
                     /locus_tag="PPY92_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626379.1"
                     /translation="MKSCPKCGQQAQDDVQICTQCGHKFDSRQALYRKSTDEDIQTNN
                     IKMRKMVPWAIGFFILILIIILFFLLRNFNSPEAQTKILVNAIENNDKQKVATLLSTK
                     DNKVDSEEAKVYINYIKDEVGLKQFVSDLKNTVHKLNKSKTSVASYIQTRSGQNILRV
                     SKNGTRYIFFDNMSFTAPTKQPIVKPKEKTKYEFKSGGKKKMVIAEANKVTPIGNFIL
                     GTYRIPAMKSTENGDFAGYLKFDFRQSNSETVDVTEDFEEANITVTLKGDTKLNDSSK
                     KVTINDREMAFSSSKTYGPYPQNKDITISASGKAKGKTFTTQTKTIKASDLKYNTEIT
                     LNFDSEDIEDYVEKKEKEENSLKNKLIEFFAGYSLANNAAFNQSDFDFVSSYIKKGSS
                     FYDDVKKRVSKGSLMMISSPQIIDAEKHGDKITATVRLINENGKQVDKEYELEQGSQD
                     RLQLIKTSEK"
     gene            2264..2392
                     /locus_tag="PPY92_002408"
     CDS             2264..2392
                     /locus_tag="PPY92_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626380.1"
                     /translation="MEYKQHPFKVFTISVNYFEGIVFCEKIYGVLRFISFYTFYKL"
     gene            2502..>3652
                     /locus_tag="PPY92_002409"
     CDS             2502..>3652
                     /locus_tag="PPY92_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HDJ2626381.1"
                     /translation="MNDLTHFNKRSPIFIIILGSLTAIGALSIDMFLPGLPDIRHDFQ
                     TTTSNAQLTLSMFMIGLAFGNLFAGPISDSTGRRKPLIIAMIIFTLASLGIVFVHNIW
                     LMVALRFLQGVTGGAAAVISRAIASDMYSGNELTKFMALLMLVNGIAPVVAPTIGGII
                     LNYSVWRMVFVILTIFGFVMVIGSLLKVPESLTVTNRESSSGLKTMFKNFKILLKTPR
                     FVLPMLIQGMTFVILFTYISASPFIIQKIYGMTAIQFSWMFAGIGITLIISSQLTGYL
                     VDFIDSQKLMRGMTMIQIIGVILVTIVLLNHWNFWILAIGFIILIAPVTGVATLGFTI
                     AMDESSSGRGSSSSLLGLVQFLFGGVASPLVGVKGEDNPIPYIIIIIATA"
BASE COUNT         1338 a          453 c          677 g         1184 t
ORIGIN      
        1 agatgattta atttattaaa gcaaggagaa cgcagcaaca tgtaagtctt aatttgttat
       61 attatatatg ggtcaatatt tttgtgtttt ttagatgtgc gatacaatga ttgctgagga
      121 tactttataa aaatagtaat tagggagaaa taattatggt aaaacattta caagaccata
      181 ttcaattttt agagcagttt ataaataacg ttaacgcatt aactgcaaaa atgttgaaag
      241 atttacaaaa tgaatatgaa atttcattag agcagtctaa cgtattaggt atgttaaata
      301 aagaaccttt gacaattagt gaaatcacgc aaagacaagg agtaaataag gccgcagtaa
      361 gccgacgaat taaaaagtta atcgatgcta aattagttaa gttagataaa ccaaatttaa
      421 atattgatca acgtttgaaa ttcataacct taactgacaa aggtagagca tatttgaaag
      481 aacgtaatgc gattatgaca gatattgcgc aagatattac taatgattta aattctgaag
      541 atattgaaaa tgtaaggcaa gtattagaag tcatcaatca tcgcattaaa acatatagca
      601 atcataaata ggtttttgaa ggtgttcaaa tataagacta aatatgtatg tattgaacac
      661 cttcttttta gttataattg agagagtact ttcataaata atgtaataag gtacaattct
      721 aaagtaaagt taatcatttc ttgaattgta ttcttgggat aatatttgta aaataataat
      781 ggtaaaataa tgaaataatt tttgtgatat acatataaca attgaagagg aacagaattg
      841 aggtgaaagt atgaaatctt gcccgaagtg cggacaacaa gcacaagatg atgtacagat
      901 atgtacacaa tgtggacata aatttgatag tcgtcaagca ttatatagaa agtcaaccga
      961 tgaagatata caaactaata acatcaagat gagaaaaatg gtgccttggg caattggttt
     1021 tttcatttta atcttaatta ttatattgtt ctttttacta agaaacttca attcacctga
     1081 agcgcaaact aaaatattag tgaatgcgat tgagaataat gataaacaaa aagtagcaac
     1141 attattaagt actaaagata ataaagtaga ttctgaagaa gcgaaagtat acattaacta
     1201 tatcaaagat gaagttgggc ttaagcaatt tgtcagcgac cttaaaaata cggtacataa
     1261 attgaataag agtaagacca gcgtagcttc ttatattcaa accagatctg gtcaaaatat
     1321 attacgtgta agtaaaaatg gcacgcgtta tatctttttc gataatatga gctttactgc
     1381 acctaccaag caaccaattg ttaagccgaa agaaaaaaca aaatatgagt ttaaatctgg
     1441 tggtaagaaa aagatggtta tagctgaagc aaataaagtg acgccaatag gtaattttat
     1501 actggggaca tatagaattc cagctatgaa atcaactgag aacggtgatt ttgcaggcta
     1561 tttaaaattt gattttagac aaagtaattc tgaaacggta gatgttactg aagattttga
     1621 agaagcaaat ataactgtaa ctttaaaagg cgatacaaaa ttaaatgata gttctaaaaa
     1681 agtaactata aatgaccgtg aaatggcatt ttcaagttcc aaaacgtatg gtccatatcc
     1741 acaaaataaa gatattacca tttcagcttc aggtaaagcg aaaggtaaaa catttacaac
     1801 acagacgaag acgattaaag ccagcgattt aaaatacaat acagagataa ctttgaattt
     1861 tgacagtgaa gatatcgaag actatgttga aaagaaagaa aaagaagaaa acagcttgaa
     1921 gaacaaattg atagaattct ttgctggata ttctttagcg aataatgctg cgtttaatca
     1981 gtcagatttt gattttgtat cgtcatatat aaaaaaagga tcatcttttt atgatgatgt
     2041 aaagaagcgt gtatctaaag gaagtttaat gatgattagt tcaccacaaa ttatagatgc
     2101 tgaaaaacat ggtgataaga ttaccgcaac cgtaagatta ataaatgaaa acggtaagca
     2161 agtagataaa gaatatgagc ttgaacaagg ctcgcaagac cgcttgcaat taatcaagac
     2221 atcagaaaaa tagagtataa aacttaaata agatgcatgt gtcatggaat acaaacaaca
     2281 tcctttcaaa gtatttacaa ttagtgtgaa ttactttgaa gggattgttt tttgtgaaaa
     2341 aatttatgga gtgctgaggt ttatatcatt ttataccttt tataagttgt aatgatgttc
     2401 atgaaacttg tgagtaatgc taatatataa aagagcatat ttaaatttag tttctgttcg
     2461 tgatagttat aaactaatca ttatataaag gagatagtca tatgaatgat ttaacgcatt
     2521 ttaacaagcg ttcaccgata tttattatta ttttaggatc tttgacggcc attggagcat
     2581 tatcgataga tatgtttttg cctggattac cagatattag acatgatttt caaacaacga
     2641 cgtctaacgc acaattaaca ttatcgatgt ttatgattgg tttagcgttt ggtaatttgt
     2701 ttgctggtcc aatttcagat tcaactgggc gcagaaagcc gcttattata gctatgatta
     2761 tttttacatt agcaagttta ggtattgttt ttgtacataa tatttggctg atggttgcat
     2821 taagatttct tcaaggagtg acaggtggcg cagcagcagt tatttcaaga gctatagcaa
     2881 gtgatatgta tagtggtaat gaactaacta agtttatggc attattgatg cttgttaatg
     2941 gtattgcacc agttgttgcg ccaacaatag ggggcataat tttaaattat tctgtctggc
     3001 gtatggtctt tgtcattctg actatctttg gtttcgtcat ggtcataggt tctttattaa
     3061 aagtacctga atcattaaca gtgacaaatc gtgagtcaag tagtggatta aaaacaatgt
     3121 ttaaaaactt caaaatatta ttgaagacgc cacgttttgt attaccaatg ctcattcaag
     3181 gtatgacatt tgtaatatta tttacttata tttctgcgtc tccgtttatt attcaaaaaa
     3241 tttatggcat gactgcaata caatttagtt ggatgtttgc aggaattggt attacgctca
     3301 ttatatcaag tcaacttact gggtaccttg ttgattttat agactcacaa aaattgatga
     3361 gaggcatgac gatgattcag attatcggtg taattttagt gacgattgta ttgcttaatc
     3421 attggaactt ttggatttta gcgataggat ttatcatttt aatagcacca gtgacaggtg
     3481 ttgcgacact tggttttaca atagctatgg atgaaagtag tagtggtaga gggagttcat
     3541 caagtttatt aggtttagtg cagtttttat ttggtggtgt tgcatcgcca ttagtggggg
     3601 taaaaggtga agataatcct ataccttaca taattattat tatagctaca gc
//
LOCUS       DANBWH010000222         3638 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.230, whole genome shotgun sequence.
ACCESSION   DANBWH010000222 DANBWH010000000
VERSION     DANBWH010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.230"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..932
                     /gene="lukE"
                     /locus_tag="PPY92_002410"
     CDS             <1..932
                     /gene="lukE"
                     /locus_tag="PPY92_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_114304764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit E"
                     /protein_id="HDJ2626382.1"
                     /translation="KKKMLAATLSVGLIAPLASPIQESRANTNIENIGDGAEVIKRTE
                     DVSSKKWGVTQNVQFDFVKDKKYNKDALIVKMQGFINSRTSFSDVKGSGYELTKRMIW
                     PFQYNIGLTTKDPNVSLINYLPKNKIETTDVGQTLGYNIGGNFQSAPSIGGNGSFNYS
                     KTISYTQKSYVSEVDKQNSKSVKWGVKANEFVTPDGKKSAHDRYLFVQSPNGPTGSAR
                     EYFAPDNQLPPLVQSGFNPSFITTLSHEKGSSDTSEFEISYGRNLDITYATLFPRTGI
                     YAERKHNAFVNRNFVVRYEVNWKTHEIKVKGHN"
     gene            934..1917
                     /gene="lukD"
                     /locus_tag="PPY92_002411"
     CDS             934..1917
                     /gene="lukD"
                     /locus_tag="PPY92_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit D"
                     /protein_id="HDJ2626383.1"
                     /translation="MKMKKLVKSSVASSIALLLLSNTVDAAQHITPVSEKKVDDKITL
                     YKTTATSDNDKLNISQILTFNFIKDKSYDKDTLVLKAAGNINSGYKKPNPKDYNYSQF
                     YWGGKYNVSVSSESNDAVNVVDYAPKNQNEEFQVQQTLGYSYGGDINISNGLSGGLNG
                     SKSFSETINYKQESYRTTIDRKTNHKSIGWGVEAHKIMNNGWGPYGRDSYDPTYGNEL
                     FLGGRQSSSNAGQNFLPTHQMPLLARGNFNPEFISVLSHKQNDTKKSKIKVTYQREMD
                     RYTNQWNRLHWIGNNYKNQNTVTFTSTYEVDWQNHTVKLIGTDSKETNPGV"
     gene            complement(2287..3078)
                     /locus_tag="PPY92_002412"
     CDS             complement(2287..3078)
                     /locus_tag="PPY92_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1828 domain-containing protein"
                     /protein_id="HDJ2626384.1"
                     /translation="MHDNTIKSISKDYFDYLQTESKFIPLENETIEFYSPIVDYFGDS
                     ISVNISFSGDRYKLTDHGETLWNMEQLGIDLTSNKKQKKYQLLNNIVQNYSLVIENKH
                     INFYTNRINLSQAIHDYVLAISEISYLGILKKENIKSLFKDEVMQYFLSNRKIYPNIF
                     PEFKVEGKSKLIHSFEAVFPGEATEYVKTIKRIDKNNAKNVLFDWNDVEIHRNKNFDS
                     NARLNIITEQQDDISEAVSTMLSQYNVEVFSFENKKQLEEKFSNV"
     gene            complement(3083..3556)
                     /locus_tag="PPY92_002413"
     CDS             complement(3083..3556)
                     /locus_tag="PPY92_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626385.1"
                     /translation="MHLKLNDEDLNYLVNLSKEILEQYNIKLDETCKGDINITSCNSE
                     YFFTLSYFLKPGKATINFRECRYNYSLLRLNLNDGFHKNSNNEKIRGNRINIFSEKEF
                     IQKADGATHMKAYPLPYNIFDDSSDFVKQLFTLLEYTKTNHNDNIKIETNLFMSW"
BASE COUNT         1321 a          508 c          567 g         1242 t
ORIGIN      
        1 ttaagaaaaa aatgttagct gcaactttgt cagtaggact gattgcgcct ttagcatctc
       61 cgattcaaga atctagagct aatactaata tagagaatat tggtgatggt gctgaagtaa
      121 tcaaacgtac ggaggatgta agtagtaaga aatggggcgt tactcaaaat gttcaattcg
      181 actttgtaaa agataaaaaa tataacaaag atgctttaat tgttaaaatg caaggtttta
      241 ttaattccag aacttcattt tcagatgtga agggtagtgg atatgaattg actaaacgaa
      301 tgatttggcc attccaatat aatataggac tgacgactaa agatccaaat gttagcttaa
      361 tcaattacct tcctaaaaac aaaatagaaa ctactgatgt tggtcaaaca ttaggatata
      421 acattggagg taatttccag tcagcaccat ctataggtgg caatggctca tttaattatt
      481 ctaaaacaat tagttatacc caaaagagtt atgtcagtga agtagacaag caaaactcaa
      541 aatcagttaa atggggtgtt aaagcaaacg aatttgttac gcctgatggt aaaaaatctg
      601 cgcatgatag atatttattc gtacaaagtc caaatggtcc aacaggttca gcaagagaat
      661 attttgctcc tgataatcaa ttgccacctt tagttcaaag tggctttaat ccatcattta
      721 tcactacact atcacatgaa aaaggttcaa gtgatacgag tgaatttgaa atttcatatg
      781 gtagaaactt agatattaca tatgcgactt tattccctag aactggtatt tacgcagaaa
      841 gaaagcataa tgcatttgta aatagaaact ttgtagttag atatgaagtt aattggaaaa
      901 cacacgaaat taaagtgaaa ggacataatt aatatgaaaa tgaaaaaatt agtcaaatca
      961 tcagttgctt catcaattgc actgcttttg ctatcgaata cagttgatgc agctcaacat
     1021 atcacacctg taagcgagaa aaaagtagat gacaaaatca ctttatacaa aacaacagca
     1081 acatctgata atgataaatt gaatatttct caaattttaa cgtttaattt cattaaggat
     1141 aaaagttatg acaaagatac attagtactt aaggcagccg gaaacattaa ttcaggttat
     1201 aaaaagccta atccaaaaga ttacaattac tcacagtttt attggggcgg taagtataat
     1261 gtttcggtta gttcagaatc aaatgatgct gtaaatgttg ttgactatgc acctaaaaat
     1321 caaaatgaag aattccaagt tcaacaaaca ttaggttatt cttatggcgg agatattaat
     1381 atatctaatg gcttatcagg tggattgaat ggatcaaaat cattttcaga aacaataaat
     1441 tataaacaag aaagttacag aactacgatt gatagaaaaa caaatcataa atcaattggc
     1501 tggggtgttg aggcgcacaa aattatgaat aatggttggg gaccatatgg tagagatagt
     1561 tatgacccaa catatggtaa tgaactgttt ttaggtggta gacaaagtag ttcaaatgct
     1621 ggacaaaatt tcttgccaac acatcaaatg cctttattgg cgcgtggtaa ctttaaccca
     1681 gaatttataa gcgtactttc tcataaacaa aatgatacaa aaaaatctaa aatcaaagta
     1741 acttaccaaa gagaaatgga tagatatact aatcaatgga atcgactaca ctggattggt
     1801 aataactaca aaaatcaaaa tacagtaacg tttacatcta cttatgaagt tgactggcaa
     1861 aaccatactg ttaaattaat cggtacggat tctaaagaaa ctaatcctgg agtatagaat
     1921 tgtatatgat taaataatca acaaagtgca attatttcaa ttatcaaacg gaattaaaga
     1981 actaacataa tttttattta cacacatgat aatttttagt tagggaaatg tagattactt
     2041 tgctaaatag cagggtgcta aatttacgaa agtagaatta tttagaaact tccaacatat
     2101 aaaaaattat taaaaaaatg gaaccacgtc ttaatcggcg tggttcgttt tgtagatata
     2161 gcatctatga ttgtatgacg gtgaaagttc attacaaact tggtattggt aaatttatca
     2221 ttttaagtat cggtgattta attgaaatag aaactattat cgaagaaaca ttatataaca
     2281 cgataattat acgttactaa acttttcttc aagttgtttt ttgttttcaa atgaaaatac
     2341 ctctacatta tattgagaca acattgttga tactgcttcg ctaatatcat cttgttgttc
     2401 tgtaatgata ttaagtctag cattagaatc aaaattttta tttctatgta tttcaacatc
     2461 attccaatcg aaaaggacat tttttgcatt gtttttatcg attcgtttaa ttgttttaac
     2521 atactctgta gcttctcctg gaaatactgc ttcgaaacta tggattaact tagatttccc
     2581 ctctacctta aattcgggaa atatattagg ataaatcttt ctgtttgata gaaaatattg
     2641 catcacttcg tctttaaata aggacttaat attttctttc tttaatattc ccaaataact
     2701 gatctcggaa atagccaaaa cataatcatg aatagcttga gataagttta ttctatttgt
     2761 ataaaagttt atgtgcttat tttcgatgac tagactgtag ttttgtacaa tattattgag
     2821 aagttgatac tttttttgtt ttttattact agttaaatct atacctaatt gttccatgtt
     2881 ccataaagtt tcaccatgat cagttagttt atatcgatcc cctgaaaaag atatatttac
     2941 agaaatagaa tcaccaaaat aatcgacgat aggtgaataa aattctatag tttcattttc
     3001 taatgggata aactttgatt ctgtttgtaa gtaatcaaaa tagtctttgc taatagattt
     3061 aattgtgttg tcatgcatag tatcaccaac tcatgaatag attagtttca attttaatat
     3121 tatcattatg attagttttt gtatattcaa gcaaagtaaa taattgtttt acaaaatcag
     3181 agctatcgtc aaatatatta taaggtaaag gatatgcttt catatgggtt gcgccgtcag
     3241 ctttttgtat aaattctttt tcagaaaata tattaattct atttcccctt attttctcat
     3301 tattagagtt tttatgaaaa ccgtcattaa gatttaaacg taacaagcta tagttgtaac
     3361 ggcattctct gaaatttata gttgcttttc caggctttaa aaaatagctt aaagtgaaaa
     3421 agtattcaga attacatgat gttatgttaa tatctccctt acaagtttcg tctaatttaa
     3481 tgttgtattg ttcaagtatt tctttactta gatttactaa ataatttaaa tcttcatcgt
     3541 tcagtttcaa atgcatttaa acatctcctt agtttagatt ttacgatctt aaatccttat
     3601 attattttca gtatttgtag taagttatac agatgttt
//
LOCUS       DANBWH010000223         3593 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.231, whole genome shotgun sequence.
ACCESSION   DANBWH010000223 DANBWH010000000
VERSION     DANBWH010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.231"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(482..586)
                     /locus_tag="PPY92_002414"
     CDS             complement(482..586)
                     /locus_tag="PPY92_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626386.1"
                     /translation="MLTNRLTSVNYVKKRLIQNIQRILSKEVLYGEMQ"
     gene            681..1991
                     /gene="sbi"
                     /locus_tag="PPY92_002415"
     CDS             681..1991
                     /gene="sbi"
                     /locus_tag="PPY92_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin-binding protein Sbi"
                     /protein_id="HDJ2626387.1"
                     /translation="MKNKYISKLLVGAATITLATMISNGEAKASENTQQTSTKHQTTQ
                     NNYVTDQQKAFYQVLHLKGITEEQRNQYIKTLREHPERAQEVFSESLKDSKNPDRRVA
                     QQNAFYNVLKNDNLTEQEKNNYIAQIKENPDRSQQVWVESVQSSKAKERQNIENADKA
                     IKDFQDNKAPHDKSAAYEANSKLPKDLRDKNNRFVEKVSIEKAIVRHDERVKSANDAI
                     SKLNEKDSIENRRLAQREVNKAPMDVKEHLQKQLDALVAQKDAEKKVAPKVEAPQIQS
                     PQIEKPKAESPKVEVPQIQSPKVEVPQSKLLGYYQSLKDSFNYGYKYLTDTYKSYKEK
                     YDTAKYYYNTYYKYKGAIDQTVLTVLGSGSKSYIQPLKVDDKNGYLAKSYAQVRNYVT
                     ESINTGKVLYTFYQNPTLVKTAIKAQETASSIKNTLSNLLSFWK"
     gene            2527..3456
                     /gene="hlgA"
                     /locus_tag="PPY92_002416"
     CDS             2527..3456
                     /gene="hlgA"
                     /locus_tag="PPY92_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB subunit A"
                     /protein_id="HDJ2626388.1"
                     /translation="MIKNKILTATLAVGLIAPLANPFIEISKAENKIEDIGQGAEIIK
                     RTQDITSKRLAITQNIQFDFVKDKKYNKDALVVKMQGFISSRTTYSDLKKYPYIKRMI
                     WPFQYNISLKTKDSNVDLINYLPKNKIDSADVSQKLGYNIGGNFQSAPSIGGSGSFNY
                     SKTISYNQKNYVTEVESQNSKGVKWGVKANSFVTPNGQVSAYDQYLFAQDPTGPAARD
                     YFVPDNQLPPLIQSGFNPSFITTLSHERGKGDKSEFEITYGRNMDATYAYVTRHRLAV
                     DRKHDAFKNRNVTVKYEVNWKTHEVKIKSITPK"
BASE COUNT         1397 a          634 c          506 g         1056 t
ORIGIN      
        1 tttcttcaaa tttatttgtt agaaaggctt atgcattagt agcgatgcta tctaatgcgt
       61 tcctttattt tctgcgaatt ttatgagcta gaaaggctta tgcagttgac gttttacgtc
      121 caactgcgtt ccttcgtctt cttcaaattt atttgttaga aaggcaccca actctccctg
      181 tttgttaact ttcgtctcga aagtttctat gttgggaccc tatgcattag tagcgaagct
      241 atctaatgcg ttcctttatt ttctgcgaat tttatgagct agaaagccgc ccaactcttc
      301 ctgtttgtga actttcgcct cgaaagtttc tatgttggga ccctatgcat tagttgcgaa
      361 gctatctaat gtcgtgacct aattatatag aagaaaaagt gcatcagtga caaattaaat
      421 gagatttcta ctctaccaaa ctctcttcga aagacaattt cctcctctat ttattagcaa
      481 actattgcat ttctccatat agtacttcct tacttaaaat acgctgaatg ttctgaatta
      541 aacgcttttt tacatagtta acactagtta atctattagt taacattagt taataattag
      601 ttaatttcca tttgtattct catgtgataa attctaaaag catacaataa atttaatatg
      661 taaaaagaaa gggaatacac atgaaaaata aatatatctc gaagttgcta gttggggcag
      721 caacaattac tttagctaca atgatttcaa atggggaagc aaaagcgagt gaaaacacgc
      781 aacaaacttc aactaagcac caaacaactc aaaacaacta cgtaacagat caacaaaaag
      841 ctttttatca agtattacat ctaaaaggta tcacagaaga acaacgtaac caatacatca
      901 aaacattacg cgaacaccca gaacgtgcac aagaagtatt ctctgaatca cttaaagaca
      961 gcaagaaccc agaccgacgt gttgcacaac aaaacgcttt ttacaatgtt cttaaaaatg
     1021 ataacttaac tgaacaagaa aaaaataatt acattgcaca aattaaagaa aaccctgata
     1081 gaagccaaca agtttgggta gaatcagtac aatcttctaa agctaaagaa cgtcaaaata
     1141 ttgaaaatgc ggataaagca attaaagatt tccaagataa caaagcacca cacgataaat
     1201 cagcagcata tgaagctaac tcaaaattac ctaaagattt acgcgataaa aataaccgct
     1261 ttgtagaaaa agtttcaatt gaaaaagcaa tcgttcgtca tgatgagcgt gtgaaatcag
     1321 caaatgatgc aatctcaaaa ttaaatgaaa aagattcaat tgaaaacaga cgtttagcac
     1381 aacgtgaagt taacaaagca cctatggatg taaaagagca tttacagaaa caattagacg
     1441 cattagtagc tcaaaaagat gctgaaaaga aagtggcgcc aaaagttgag gctcctcaaa
     1501 ttcaatcacc acaaattgaa aaacctaaag cagaatcacc aaaagttgaa gtccctcaaa
     1561 tccaatcacc aaaagttgag gttcctcaat ctaaattatt aggttactac caatcattaa
     1621 aagattcatt taactatggt tacaagtatt taacagatac ttataaaagc tataaagaaa
     1681 aatatgatac agcaaagtac tactataata cgtactataa atacaaaggt gcgattgatc
     1741 aaacagtatt aacagtacta ggtagtggtt ctaaatctta catccaacca ttgaaagttg
     1801 atgataaaaa cggctactta gctaaatcat atgcacaagt aagaaactat gtaactgagt
     1861 caatcaatac tggtaaagta ttatatactt tctaccaaaa cccaacatta gtaaaaacag
     1921 ctattaaagc tcaagaaact gcatcatcaa tcaaaaatac attaagtaat ttattatcat
     1981 tctggaaata atcaatcaaa aatatcttct ctagttttac atcatttttt aaataatttt
     2041 cgtaataaac cgtgattaaa aagaaccgct gattctcaat cgaatctgtg gttctttttt
     2101 tattttccat caattaaatg tttcttcgct atttgtcagt ccacttcttt acctgcaact
     2161 tgttaaataa tccttacatc gttaacgaat agttcatcat ttagttgaat cagctcaact
     2221 ttattaactt caaattttca caaactattg cgaaatccat tcctcttcca ctacaagcac
     2281 cataattaaa caacaattca atagaataag acttgcaaaa catagttatg tcgctatata
     2341 aacgcctgcg accaataaat cttttaaaca taacataatg caaaaacatc atttaacaat
     2401 gctaaaaatg tctcttcaat acatgttgat agtaattaac ttttaacgaa cagttaattc
     2461 gaaaacgctt acaaatggat tattatatat atgaacttaa aattaaatag aaagaaagtg
     2521 atttctatga ttaaaaataa aatattaaca gcaactttag cagttggttt aatagcccct
     2581 ttagccaatc catttataga aatttctaaa gcagaaaata agatagaaga tatcggtcaa
     2641 ggtgcagaaa tcatcaaaag aacacaagac attactagca aacgattagc tataactcaa
     2701 aacattcaat ttgattttgt aaaagataaa aaatataaca aagatgccct agttgttaag
     2761 atgcaaggct tcatcagctc tagaacaaca tattcagact taaaaaaata tccatatatt
     2821 aaaagaatga tatggccatt tcaatataat atcagtttga aaacgaaaga ctctaatgtt
     2881 gatttaatca attatcttcc taaaaataaa attgattcag cagatgttag tcagaaatta
     2941 ggctataata tcggcggaaa cttccaatca gcgccatcaa tcggaggcag tggctcattc
     3001 aactactcta aaacaattag ttataatcaa aaaaactatg ttactgaagt agaaagtcag
     3061 aactctaaag gtgttaaatg gggagtgaaa gcaaattcat ttgttacacc gaatggtcaa
     3121 gtatctgcat atgatcaata cttatttgca caagacccaa ctggtccagc agcaagagac
     3181 tatttcgtcc cagataatca attacctcct ttaattcaaa gtggctttaa tccatcattt
     3241 attacaacat tgtcacacga aagaggtaaa ggtgataaaa gcgagtttga aatcacttac
     3301 ggcagaaaca tggatgctac atatgcttac gtgacaagac atcgtttagc cgttgataga
     3361 aaacatgatg cttttaaaaa ccgaaacgtt acagttaaat atgaagtgaa ctggaaaaca
     3421 catgaagtaa aaattaaaag catcacacct aagtaaacag ttcaatcatt ttaaaaaatc
     3481 ctgggacact tcatacttgt ctcaggattt tttaacatat tgaatcagcc tcataacatt
     3541 aaattaattt atcgtacatt aaatttaata ataacaactg atttttataa gaa
//
LOCUS       DANBWH010000224         3587 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.232, whole genome shotgun sequence.
ACCESSION   DANBWH010000224 DANBWH010000000
VERSION     DANBWH010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.232"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..456)
                     /gene="deoC"
                     /locus_tag="PPY92_002417"
     CDS             complement(<1..456)
                     /gene="deoC"
                     /locus_tag="PPY92_002417"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDJ2626389.1"
                     /translation="MKFEKYIDHTLLKPESTRTQIDQIIDEAKAYNFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVVKAAKGHTVKVIIETVLLDHDEIVKASELTKAAGADFVKT"
     gene            complement(537..1889)
                     /gene="tet(38)"
                     /locus_tag="PPY92_002418"
     CDS             complement(537..1889)
                     /gene="tet(38)"
                     /locus_tag="PPY92_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HDJ2626390.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFPLL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVILNRWTQSEK
                     "
     gene            complement(1896..2603)
                     /gene="deoD"
                     /locus_tag="PPY92_002419"
     CDS             complement(1896..2603)
                     /gene="deoD"
                     /locus_tag="PPY92_002419"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDJ2626391.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            2839..>3587
                     /locus_tag="PPY92_002420"
     CDS             2839..>3587
                     /locus_tag="PPY92_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDJ2626392.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLARV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPRFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQS"
BASE COUNT         1222 a          671 c          550 g         1144 t
ORIGIN      
        1 agttttaacg aagtccgcac cagccgcttt tgttaattca ctcgctttta caatttcgtc
       61 atggtccaac aataccgtct caataatcac ttttactgtg tgacctttcg cagctttaac
      121 cactgcttca atgtcttgtt gtacatcatc aaaacgtcca tcttttaatg cgccgatgtt
      181 gatgaccatg tcaatttcat ctgcaccatt ttgaattgca tcttctgttt caaatgcttt
      241 cgttgcagtt gtcgacgcac ctaatgggaa tcctattacc gtacaaacga gcacctctga
      301 atcagctagt cgctctgctg catatttaac atgcgttgga ttcacacata cagatttaaa
      361 attgtatgct ttcgcttcat cgatgatttg atcgatttgc gtacgtgttg actcaggctt
      421 caataaagtg tgatctatat atttctcaaa tttcatactt actactcctc gtgttatata
      481 atctctttat ttaattttac tataaatacg aatatatatc gcgaatttat atctatttat
      541 ttttcagatt gtgtccaacg atttaagatg acgaataaaa ctaatccgac aatactcatt
      601 aaacctaaaa taattaaaat aataccgaaa tgtgaactta atgcatcatt gcctgggaaa
      661 tttaatgctt taaaatcgat tagagccgca gcaatcgcaa tacctacaga taccgccaca
      721 ttaataatta aattataaaa accaatagcc acacctgtca tattaagatc tattgtttta
      781 atggcttcgt taagtaaagg tgcatacatt aaagcaaagc tacctgcaaa gaatatcata
      841 gaaatgacga agattgaaat gtgattacct actgcaaatg caggtaaaat caagctcagt
      901 gctattaaaa taattgctgt gataatcgct tgttttgaat tcagatattc gccgatttta
      961 ccacttaatg caccaacaat gactgctact atataacccg gtactaataa cagtgatgtt
     1021 gtgtctagtt gcagatgata aatttgctcc attatgaatg ggaacgtaaa aatataaccc
     1081 aattggatag catacattac aaatactata aataaaaatg aagcataacg tttattttgg
     1141 aaaaatgatt tatttactaa tggacgttgc gcatttttaa tatatagcgc aaaaacgata
     1201 atcgcaatta aggcaccaat catatataac caattaaagt tcgtaataaa cagcatgact
     1261 gttgtagcaa tagttgccac tagaattaag ccaacaaaat ctaaatgagc tttattcgta
     1321 ttattttctt ttggtaataa tttaaatagg aatggcaacg taaatactac gattaatgca
     1381 attaaaaaca tatttgtcca gtgtaaatac gtagaaataa atccacctga taatgtaccg
     1441 ataactaatg acaaggaata actgctcgta cttaagccaa ggtaagtctt ctggtcctct
     1501 ttagaaagat actttgcaac atatatcaca tataatgtct ctgcagcagc taaaccggca
     1561 gtttgaataa tacgtccaac taaaagtaat gggaattgat gttggaaaat gtaaccaata
     1621 attgatccga taatgattaa tatcacgcca taaataaata gtgtgcgtat actaatggca
     1681 tcagataatg aagcgtatac tacagcgcca ataccaatta ctaaaccggc taatgtcgct
     1741 tgccaactta ctgtcgttac agatatgttt aagtcatcag caatggctac agaaatcaat
     1801 ttaaatgagt tgtctataac caaactgaat acaaataaga ataataaaat aggtactgct
     1861 ttctttattt tagaatattc aacattcata atcatctaca ccaatgacag tgcaatttca
     1921 atcatatctg taaatgcacg ttccctttcc tcaggtgttg ttgacgtttc atgaattaaa
     1981 tgatcgctca ctgtgaacac acctaatgct tcgacaccag cgtaaattgc attcatgtat
     2041 aatgcagctg attccatttc tacacctaaa atacccatac gcatccaacg ttcactcgct
     2101 gttgtatccg cgttatagaa aatatcactt gataacacat tacctacatg atgacgtaca
     2161 cctttgtcac gtgctgtttc aactgctttt tctaataatt gataagaagc aattggcgca
     2221 aaatgacctg gtaattgata ttgttgaacg taatttgaat cagtagaggc accttgtgca
     2281 ataatcacat catataaatc aatgttttct tgcatcgcgc cacaagagcc aacgcgaatt
     2341 aattttttac aaccaaatgt atgaattaat tcataagagt aaatgccaat agatggcata
     2401 cccatacctg aacccatgac agaaactttt ttacctttat atgttccggt aaaaccaaac
     2461 atgtttcgca ctgtattgaa ctgttccaca tcatccaaat aagtttctgc aatgaactta
     2521 gctcttaacg gatctcctgg caatagaacc gtttctgcaa tttcgacgtc attcattggt
     2581 ttaatgtgtg gtgttgattt cataaaatat tcctcccaat atttgtatag tgatttgtct
     2641 atttgtctaa tcatgtatat aataatattt tttaaaagcg ctttcaagca ttacattttg
     2701 acaatattaa gaacatttta cagttacagc tactaacctt gtataaaact aattttcaaa
     2761 gcctatctat atttaatttt tataattttt ccaattcgag taaaaattat tgtatgatga
     2821 gagtgattaa gtaactttat ggaggttgtc tacgtgacgc aaacacaacc aaaattttta
     2881 acgatttata atacgttata taaagaaatt caaataggaa aatatccgag tggccatgca
     2941 ttacctactg aaaaggaact ttgtgcgcgc tttgatgtca gtcgtatgac tttgcgccaa
     3001 gctataaaat tgttagccga agatggcatc gtagagagta caagagggaa gggacacttt
     3061 gttattccac aaacaaatgc ccaacacgct tcaagtatgg atcaatttaa gcatccactt
     3121 gatcaaatat tgctagctcg cgtatctatg tcatcaatta attatcgtgt tgatttagaa
     3181 agtgaatata caaatcattt gtttgcaaac catccttctg ccgtcattgc aatggaacgc
     3241 tattatcaaa aaaaggacaa tcattctaag caagctgatg cattttgctt tacctttata
     3301 ccgttaaatg tcattgacac atttaaagtg aatacacaaa acgaagacga catgaagaca
     3361 ttcgttgagc aaacgattta tagcaatgca tatcaatcag atttaaaaat gtctattacc
     3421 aaagcgcccc gcttcaaaaa tcatagtcac gtgtttgatg gtgatacaca ttgttggctg
     3481 attattgaaa cactgtacgc acaaacacct tatccaatta tgattaataa gtggtatatt
     3541 ccacaggaaa tatctgaatt aacactgaca cgtataagac aatcgga
//
LOCUS       DANBWH010000225         3569 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.233, whole genome shotgun sequence.
ACCESSION   DANBWH010000225 DANBWH010000000
VERSION     DANBWH010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.233"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..230)
                     /locus_tag="PPY92_002421"
     CDS             complement(<1..230)
                     /locus_tag="PPY92_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626393.1"
                     /translation="MKKSIYILPLAYIVIESPLQIGNVILTPSLKVIESGHFHTDFSL
                     ISMFNKKEFLSILSTSQLKEDALVIFKDDFFEP"
     gene            1075..1401
                     /locus_tag="PPY92_002422"
     CDS             1075..1401
                     /locus_tag="PPY92_002422"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDJ2626394.1"
                     /translation="MKVKLRLTCVYSAFRAYVLKHVVFNRYFMKGYQQMSPKGTTRIE
                     TKTGHQAQFDWKEGINFKTNDNQIVSLNIGVLLLSYSRFVIMKVTMNKSSDVLFNLFT
                     QAFELM"
     gene            1885..2603
                     /locus_tag="PPY92_002423"
                     /pseudo
     CDS             1885..2603
                     /locus_tag="PPY92_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066123.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            3348..>3569
                     /locus_tag="PPY92_002424"
     CDS             3348..>3569
                     /locus_tag="PPY92_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDJ2626395.1"
                     /translation="MRAFTEANKKSKYQEQTNNARKSEASNCPICNTDLQYDHQTHIW
                     NYKDMVGDHIIPWSKGGKTERGNLQMLYKH"
BASE COUNT         1388 a          456 c          557 g         1168 t
ORIGIN      
        1 ggttcaaaga aatcgtcctt aaaaataact agtgcatctt cttttaattg cgaagtagat
       61 aaaatagata aaaactcttt tttgttaaac attgatatta gtgaaaagtc tgtatgaaaa
      121 tgacctgatt caattacttt taaagatggg gttaagataa cattgccaat ttgtaaaggt
      181 gactcaataa ctatataagc taatggtaag atataaatac tctttttcat tataaaatcc
      241 tcctaaaaat taatatgttt ttgaaattca tctgccaatt tttctctaga tgattttgta
      301 atgtgtttat aaatgtccat agtcgtcttt atatcactat gacctaatct agttttactt
      361 ctttaagttg ttgcacctga ttcgaataat agtgaagcac gagtatgcct gaagctatga
      421 acgtgtatct tatgaagatt tgaatatcat ttaaagaata cctctaattc gtcattaggc
      481 atcgatacac gcatgtactt catttagtct actatgtatg gtatgttgaa aagatatgtt
      541 gctctttatc actagagact gaaaatccat attcttcatt ttgtagattt tgttcttctt
      601 tccattgtaa aagtttattt atagtttgaa taccaatatc tatccatctt tgatactcac
      661 atttttttag tggttgcaat acatgtttat ttttcactgg aaattataat ttaataatgt
      721 acacttatga ttgtttaaac atgtaaaatt tttaaaaacc tagagataat tttattgagg
      781 cgaacaacaa tgaaattatc tttagacata aatatggatt tttaagttat tactcttaca
      841 gatttaccaa aattaaaaat tgttaagcag aacttaatca tgaaataaac aaaagtgaaa
      901 tagcaagaca tatgggggat gatagaagaa cagttgataa ataattaaat ggttttgagc
      961 cgaccaagaa gaggaaccgt cagtctatta ttgacaagta ttatccaatc atccaaaagt
     1021 tactttcaca ctagtgaata atagttctac tataatggtg tattttgaca atatttgaag
     1081 gttaaactta gattaacctg tgtctattct gcatttagag cttatgtcct taagcatgtt
     1141 gtatttaatc gttattttat gaaaggttat caacagatgt cgcctaaagg tacaacgaga
     1201 atcgagacaa aaaccggtca tcaagcacag tttgactgga aagaaggcat taactttaaa
     1261 acgaatgata atcaaatagt ttcactaaat ataggcgtgt tactactttc ttactcccgt
     1321 tttgtaatca tgaaagtgac aatgaataaa tcgagtgatg ttttatttaa tttatttaca
     1381 caagcgtttg agctaatgtg agatgtgcct gatgtacttg taactgatag tattatattt
     1441 agaagaggtt cctaagttta tttctaatct taaagagcga ctgaactact cagttcatgt
     1501 gagtacaggt aaaatgccta ttattgcctt agaaaaagaa aaagttctta caatcactac
     1561 cgaatgtgaa tgtaagaaac tcatataaag tgaaacataa atatttgaaa gtcaaccgct
     1621 ctaatatgat tacatataag tccaatcaat attcagcatc agctgaatat tgtgggaaaa
     1681 cagttgaagt tcaagtttat gatcaaaaac aacacgttta atataagact aaattgattt
     1741 gtgaacatct catcacacaa cgtaaattaa attatcaaaa agaacattat ttagaatcac
     1801 tgccatcctc ttttgggaat agagaaaagg atgatattaa tcagattacg atagataatt
     1861 taaatgcgat tggatagttt tatgatgaat aattcaacga attataagcg cttaaaagca
     1921 aatttagaat atttgaaaat gaatcagatg attaaccatc ttgatgatgt tatcgatttt
     1981 ataaacacaa cctgtcattc atagacacat taattaaatt aagcgattac aaaatcaaaa
     2041 ttaaggagaa aaatttgatt gaatcgatgg taaaggttat ggcgttaccg tttaaaaaga
     2101 aatatcagcc tttgacttta attttcaacc gagcatcaat caacaagatg ttttggattt
     2161 tacggattta cgttttatag gaaaatatga aaacatagtc tttttagact caaatgtcgt
     2221 tagtaaaaat agaataagta cttactttat taagtgtcat catttgaaag agaatttaag
     2281 aaaagcaaaa cttgagaaca gcttagaaaa tagattgaag cactatagca agtataagtt
     2341 attaatcatt gatgagattg ggtacttacc tattggaagt gaagctgcca aaatattctt
     2401 ccaaattaat agatttaagg tatgagaaga aaagcacaat atttaccaca aatatcaatt
     2461 tcaacttata aaatgaaata tttgatgatc ccaaaatcgc aaatgcgatt ttggatagga
     2521 tattgcatca ttcaagtgta gtaaaaatca caggaaaatc gtatagatta aaaaatcatt
     2581 cacctaaaaa gaaaattact tagcaaacaa ttttgtacat ttctatacaa tcatttatgt
     2641 agattttaat attgacattt acatccaact aaatgtagac gattcacaga caaccgatat
     2701 aattaatttg tccatccatt ttaggtggca ttttatcatg aggagtatag ttttgattgc
     2761 aaaaaaacaa atattagaac atttatcgtg aagaaaaaat gatatacttg aagaaaagta
     2821 taaatataaa aagtgaagat atttataaga ggagaaagct aatggctaat acaaaacaaa
     2881 tccattaaat caacaagaac ttagaaatgc aatctagtca ggtccattta taacaaaagc
     2941 aaagtatgaa ttttctaatt ctatgaatgc taatcagcaa aaaatggcaa agttatatta
     3001 aaggaattcc aaatagataa gaaatattag aagaagctct aaatggggga agtaaaggtg
     3061 atattaatgg atacatgtca agacatcata atgatataaa tgatttaaaa acatacttta
     3121 actcagttat agattggata tcgagcattt ttattaatgt agaaaaagaa atgcggggat
     3181 tagaatagag aagtttatat gaaacatatc ataacaactc ttaaaatcct tcagtattag
     3241 aatataaact tttaagttta tatgaagata gtgatgtttc aaggaagcta ggaatttttg
     3301 agtatttatt aagcggagaa aaattagaat ttataaaata actatcaatt agagctttca
     3361 ctgaagcgaa taaaaagtct aaatatcaag agcaaactaa taatgctcga aaaagtgaag
     3421 cttcaaattg tcctatttgt aatacagatt tacaatatga tcaccaaaca catatatgga
     3481 attataaaga tatggtagga gatcatatta taccatggag taaaggtgga aaaacagagc
     3541 gtggaaactt acagatgctt tataaacat
//
LOCUS       DANBWH010000226         3533 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.234, whole genome shotgun sequence.
ACCESSION   DANBWH010000226 DANBWH010000000
VERSION     DANBWH010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.234"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            85..891
                     /locus_tag="PPY92_002425"
     CDS             85..891
                     /locus_tag="PPY92_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDJ2626396.1"
                     /translation="MKIGTIADLHIDRHNKKTSEDYLEALVEIVKYKKLDILLIAGDI
                     SNHYQLTHQFITQLTKQLDIPVKFVPGNHDLWEVESMTTQDIWNNYKSMSQCLVGKPF
                     IVNEEWAIIGHTGWYDYSFAAQRFSLEHLQKGKHYGATWQDKERISWGISDQNLSKIA
                     AEQVKKDILEVGNRRVILVTHVVTHPDFIVPMPHRIFDFYNAFIGTSDFNPLYAMFDI
                     PYSIMGHVHFRKSVMDDGRCYLCPCLGYPRQWRSEDIYQEINETIQIIEI"
     gene            complement(954..1610)
                     /locus_tag="PPY92_002426"
     CDS             complement(954..1610)
                     /locus_tag="PPY92_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDJ2626397.1"
                     /translation="MNRKILPVKPISQLFPIPMVMGCEGVSAAMMLQYNNQHIPATEI
                     MRHWPTHPNNPHKGYVGHHLFIKLGNYHQTIFPEAYVPFLQKYNPNIVDGTGKSLEDL
                     KGIIDQGQPVLIYHTNLGSKPLLRVFRFDNKPAKQVSNIHVTLLIGYDDYYYYYIDPL
                     WSHIRRGLVLPAIIPNRKQIIKIRKEKMEYSFNSPGRKCIYVQPHSYTIENQQQNKHT
                     "
     gene            1723..2196
                     /locus_tag="PPY92_002427"
     CDS             1723..2196
                     /locus_tag="PPY92_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDJ2626398.1"
                     /translation="MEYQLQQLASLTLVGIKETYENGRQAQQHIAGFWQRCYQEGVIA
                     DLQLKNNGDLAGILGLCIPELDGKMSYMIAVTGDNSADIAKYDVITLASSKYMVFEAQ
                     GAVPKAVQQKMEEVHHYIHQYQANTVKSAPFFELYQDGDTTSEKYITEIWMPVKG"
     gene            complement(2319..3530)
                     /locus_tag="PPY92_002428"
     CDS             complement(2319..3530)
                     /locus_tag="PPY92_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HDJ2626399.1"
                     /translation="MQSTKTKTKHFSFLLLITLCVMTAFGPLTIDMYVPSLPKVQGDF
                     GSTTSEIQLTLSFTMIGLALGQFIFGPLSDAFGRKRIAVSILIIFILVSGLSMFVDQL
                     PLFLTLRFIQGLTGGGVIVIAKASAGDKFSGNALAKFLASLMVVNGIITILAPLAGGL
                     ALSVATWRSIFTILTIVALIILIGVASQLPKTSKDELKQVNFSSVIKDFGSLLKKPAF
                     IIPMLLQGLTYVMLFSYSSASPFITQKLYNMTPQQFSIMFAVNGVGLIIVSQVVALLV
                     EKLHRHILLIILTIIQVVGVALIILTLTFHLPLWVLLIAFFLNVCPVTSIGPLGFTMA
                     MEERTGGSGNASSLLGLFQFILGGAVAPLVGLKGEFNTSPYMIIIFITAILLVSLQII
                     YFKMIKKQHVA"
BASE COUNT         1238 a          521 c          698 g         1076 t
ORIGIN      
        1 tctaaatgaa gcagttaact atatatatta gataagctat ggcataataa ataacatttt
       61 aatttgagta aagaaggtat gggtatgaag atagggacaa tcgccgattt acatattgat
      121 cgtcataata aaaaaacatc cgaagattac ttagaggcat tggttgaaat agttaagtat
      181 aagaaattgg atatattgtt gattgcggga gatatctcaa atcattatca attgacgcac
      241 caatttataa cgcaattgac aaaacaatta gatatacctg tgaaatttgt tcctggaaac
      301 catgatttat gggaagttga aagtatgact acgcaagaca tttggaataa ttataagagc
      361 atgtcacagt gcttggtagg aaaaccattt atagtaaatg aagaatgggc aataatagga
      421 catacaggtt ggtatgatta tagctttgca gcacaacgat tttcattaga acatttacaa
      481 aaaggaaaac attatggtgc gacttggcaa gataaagaac gaatatcttg gggcatatca
      541 gatcaaaatt tatctaaaat agcggctgaa caagtgaaga aagatatatt agaagtagga
      601 aatagacgag tgattttagt cacacatgtt gtgacgcacc ctgatttcat tgttcctatg
      661 ccacatcgta tattcgattt ttataatgca tttattggga caagtgattt caatcctttg
      721 tatgcgatgt tcgatatacc atatagtatt atgggccatg ttcattttcg taaaagtgtg
      781 atggatgatg gcagatgtta tctctgtccg tgtctaggct atccaagaca atggcgttca
      841 gaagatattt accaggaaat aaatgagacg atacaaataa tagaaattta aaatgcgcaa
      901 acctgaccca gtttgcgcat tttatgtttt acacacgcga gtaatgtgtt tacttacgtg
      961 tgtttatttt gttgttgatt ttcaattgta tatgaatgtg gttgcacata aatgcatttt
     1021 cttcctggtg aattaaagct gtattccatt ttctctttac gaattttaat aatttgtttg
     1081 cgatttggaa tgatggcagg taaaactagg ccacgacgaa tatgactcca aagcggatcg
     1141 atataatagt aatagtaatc atcgtaccca atgagtaacg ttacgtgaat atttgaaact
     1201 tgtttcgctg gcttgttatc aaagcggaaa acacgtagta atggtttaga gccaagatta
     1261 gtatggtata ttaacacagg ttgaccttga tcgataatac ctttaagatc ttctaaggat
     1321 ttaccagtgc cgtctacgat attaggattg tatttttgta aaaatggtac atatgcttct
     1381 ggaaatatcg tttgatgata attgccaagc ttaatgaata agtgatgtcc aacataacct
     1441 ttatgtggat tgttcggatg tgtcggccaa tgtctcataa tttctgtagc agggatatgt
     1501 tggttattgt attgcaacat catggctgcg gaaacacctt cacaccccat gaccataggg
     1561 ataggaaata gctgactgat aggtttaact ggtaatattt ttcggttcat aatatagtcc
     1621 tcgcattgat tcaataaata tttaatataa ttatatagcg tcaatgcaaa atgtcctaaa
     1681 catatgtttt acatgagtga ataaaattaa tggagtgata aaatggaata tcaattacaa
     1741 caacttgcgt cgttaacgtt agtaggtatt aaagaaacgt atgaaaatgg acgacaggct
     1801 cagcaacata tagccgggtt ttggcaaagg tgttatcaag agggagtaat tgcggattta
     1861 cagttaaaaa ataatggtga tttagccggg atacttggct tatgtatacc cgaattagac
     1921 ggtaagatgt catatatgat tgcagttacc ggagataata gtgctgatat tgcaaaatat
     1981 gatgtcataa cattagcaag ttcaaagtat atggtatttg aagcacaggg cgcagtacct
     2041 aaagcagttc aacaaaaaat ggaagaggtt catcactaca tacatcaata tcaagcaaat
     2101 acggtaaaat cagcaccatt ttttgagttg tatcaggatg gtgatactac aagtgaaaaa
     2161 tatattacag aaatttggat gcctgttaaa gggtgattga atagaaatgt acttgatatg
     2221 aaggcgtaat tttagttgaa gattggattt cataattcat aaagaagaac cccgacaaag
     2281 atagatatct ttgctagggt tctaattatg ttgaagtgtt atgcgacatg ttgcttttta
     2341 atcattttaa agtaaatgat ttgtagactg actaatagaa tggctgtaat gaagataata
     2401 atcatatatg gtgatgtatt aaattcgcct tttaagccaa ctaatggtgc aacagcgcca
     2461 cctaagataa attggaataa gccaagtaaa cttgatgcgt taccactgcc acctgttcgt
     2521 tcttccatag ccattgtgaa accaagtggt ccaattgacg tcacaggaca cacatttaag
     2581 aagaatgcga tgagtaagac ccaaagtggt aaatggaatg taagtgtcag gataattaaa
     2641 gcaacaccca ccacttgtat aatagttaaa atgattaata atatgtggcg atgtaatttt
     2701 tctactaata aagcaacgac ttgactgaca atgattaaac ctacaccgtt aacagcaaac
     2761 atgatactaa attgttgggg tgtcatatta tacaattttt gagtaataaa tggcgatgca
     2821 gatgaataac taaaaagcat tacataagtt aaaccttgta atagcattgg aataataaat
     2881 gctggttttt tcaaaagact tccaaagtct ttaatgacgc tactaaaatt cacctgcttt
     2941 aattcatctt tagatgtttt aggtaattga gaagcgacgc caattaaaat gatgagtgcc
     3001 acaatagtta aaattgtgaa aatagaacgc catgttgcta cggataaagc taatccacca
     3061 gctaatggtg caagaatagt gatgatgcca tttactacca ttaaagatgc taaaaattta
     3121 gcgagtgcgt tgccactaaa tttatcacca gcagaggctt tggcaatcac gatgacgcca
     3181 ccaccagtta aaccttgaat aaatcgtaaa gttaagaata atggcaattg atcaacaaac
     3241 atagacaaac ctgataccaa aatgaaaatg atcaaaatgg atacagcaat ccgtttgcga
     3301 ccaaaagcat cggataaagg tccaaagata aattggccta gtgcaagacc aatcattgtg
     3361 aatgataatg ttaattgaat ttctgatgta gtagaaccaa aatcaccttg cactttaggt
     3421 aatgatggta cgtacatatc tatagttagt gggccaaaag cggtcatgac gcataacgta
     3481 attagcaata aaaatgaaaa atgcttcgtt ttggttttcg ttgattgcat gat
//
LOCUS       DANBWH010000227         3527 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.235, whole genome shotgun sequence.
ACCESSION   DANBWH010000227 DANBWH010000000
VERSION     DANBWH010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.235"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(75..1319)
                     /gene="fabF"
                     /locus_tag="PPY92_002429"
     CDS             complement(75..1319)
                     /gene="fabF"
                     /locus_tag="PPY92_002429"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDJ2626400.1"
                     /translation="MSQNKRVVITGMGALSPIGNDVKTTWENALKGVNGIDKITRIDT
                     EPYSVHLAGELKNFNIEDHIDKKEARRMDRFTQYAIVAAREAVKDAQLDINDNTADRI
                     GVWIGSGIGGMEIFEIAHKQLMDKGPRRVSPFFVPMLIPDMATGQVSIDLGAKGPNGA
                     TVTACATGTNSIGEAFKIVQRGDADAMITGGTEAPITHMAIAGFSASRALSTNDDIET
                     ACRPFQEGRDGFVMGEGAGILVIESLESAQARGANIYAEIVGYGTTGDAYHITAPAPE
                     GEGGSRAMQAAMDDAGIEPKDVQYLNAHGTSTPVGDLNEVKAIKNTFGEAAKHLKVSS
                     TKSMTGHLLGATGGIEAIFSALSIKDSKVAPTIHAVTPDPECDLDIVPNEAQDLDITY
                     AMSNSLGFGGHNAVLVFKKFEA"
     gene            complement(1331..2272)
                     /locus_tag="PPY92_002430"
     CDS             complement(1331..2272)
                     /locus_tag="PPY92_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDJ2626401.1"
                     /translation="MNVGIKGFGAYAPEKIIDNAYFEQFLDTSDEWISKMTGIKERHW
                     ADDDQDTSDLAYEASVKAIADAGIQPEDIDMIIVATATGDMPFPTVANMLQERLGTGK
                     VASMDQLAACSGFMYSMITAKQYVQSGDYHNILVVGADKLSKITDLTDRSTAVLFGDG
                     AGAVIIGEVSEGRGIISYEMGSDGTGGKHLYLDKDTGKLKMNGREVFKFAVRIMGDAS
                     TRVVEKANLTSDDIDLFIPHQANIRIMESARERLGISKDKMSVSVNKYGNTSAASIPL
                     SIDQELKNGKLKDDDTIVLVGFGGGLTWGAMTIKWGK"
     gene            2567..2752
                     /locus_tag="PPY92_002431"
     CDS             2567..2752
                     /locus_tag="PPY92_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HDJ2626402.1"
                     /translation="MKHLVTFFWALLLMQMVNFVLNSLVGGDSLNVVNPIIMAVLFTI
                     FTAIFAAVIKPPKDSSQ"
     gene            complement(3009..3443)
                     /locus_tag="PPY92_002432"
     CDS             complement(3009..3443)
                     /locus_tag="PPY92_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HDJ2626403.1"
                     /translation="MKLKSFITVTLALGMIATTGATVTSNEVSAAEKDKLPATQKAKE
                     MQNVPYTIAVDGIMAFNQSYLNLPKDSQLSYLDLGNKVKALLYDERGVTPEKIRNAKS
                     AVYTITWKDGSKKEVDLKKDSYTANLFDSNSIKQIDINVKTK"
BASE COUNT         1076 a          689 c          518 g         1244 t
ORIGIN      
        1 tgtcgctcat cttagtataa agaaacagaa aaaatcttca gtaactaaaa caacgttact
       61 gaagattttt ataattatgc ttcaaatttc ttgaatacta atactgcgtt atgtccaccg
      121 aatcctaagc tattactcat tgcataagta atatcaaggt cttgcgcttc atttggaaca
      181 atatccaaat cacattctgg gtctggtgtt accgcatgta ttgtcggtgc gactttagag
      241 tctttaattg aaagcgctga gaagattgct tcaattccac ctgttgcacc aagtaagtga
      301 ccagtcattg attttgttga gctaactttt aagtgtttag ctgcttcacc aaatgtattt
      361 ttaatagctt taacttcatt taagtcacca acaggagtac ttgtaccatg ggcatttaag
      421 tattgtacat ctttaggttc aataccagca tcatccatag ctgcttgcat tgctctagaa
      481 ccgccttcac cttctggagc tggcgctgta atatgataag catcacctgt agtaccatag
      541 ccaactatct cagcataaat attggcacct cgagcttgtg ctgattctaa agattcgatt
      601 actaaaatac cagcaccttc acccataaca aaaccgtctc taccttcttg gaatggacga
      661 catgctgttt caatgtcatc atttgtagaa agcgctcgac ttgcactgaa acctgcaatt
      721 gccatatgag tgattggagc ttccgtacca ccagtaatca ttgcatctgc atcaccgcgt
      781 tgcacaattt taaatgcttc tccgattgag tttgtacctg ttgcacatgc tgtaactgtt
      841 gcgccatttg gtccttttgc acctaagtca attgatactt gcccagttgc catatcagga
      901 attaacattg gtacgaaaaa tggactcaca cgtcttgggc ctttatccat taattgttta
      961 tgtgcaattt caaatatttc cataccaccg ataccagaac caatccatac accgattcga
     1021 tctgcagtat tatcattgat atctaattgc gcatctttaa cagcctctct agctgctaca
     1081 attgcatatt gagtaaatct atccatacga cgcgcttctt ttttgtcgat atgatcttca
     1141 atattaaagt ttttaagttc tcctgctaag tgaacgctat aaggttcagt atcgatacgt
     1201 gtaattttat cgataccatt tacgcctttt agagcattct cccatgttgt tttgacatca
     1261 ttaccgattg gagaaagggc tcccatacct gtaataacta ctcttttatt ttgactcatt
     1321 cgttatcctc ctattttccc cattttattg tcattgcgcc ccaagttagg ccgccaccga
     1381 atccgacaag aacaattgta tcatcatctt tgagtttacc attttttaat tcttgatcga
     1441 tacttaaagg tattgacgca gctgaagtat ttccatattt atttacagaa acactcattt
     1501 tgtcttttga aatacctaag cgttctctag ctgattccat aattctaata ttagcttgat
     1561 gaggaataaa taaatctata tcatctgatg ttaaattcgc tttttcaact acacgtgttg
     1621 atgcatcacc cataattcta acagcaaatt taaatacttc tcgaccattc attttcagtt
     1681 taccagtatc tttatctaaa tataaatgtt taccaccagt gccatcagaa cccatttcat
     1741 aacttataat acctctgcct tctgaaactt caccgatgat aaccgcacct gcaccatctc
     1801 caaatagaac tgcagtagaa cggtcagtta aatctgttat tttagataat ttatctgcac
     1861 cgacaactaa aatattatga taatctccag attgaacata ttgtttagct gtaatcattg
     1921 aatacataaa tccagaacat gctgcaagtt gatccataga ggcaactttg cccgtcccta
     1981 aacgttcttg caacatattt gcgacagttg gaaatggcat atctccagtt gctgtggcaa
     2041 caattatcat atctatatct tcaggctgaa taccagcgtc agcgattgct tttacacttg
     2101 cttcatatgc taaatctgaa gtatcttgat cgtcatctgc ccaatgtctt tctttaattc
     2161 cagtcatctt agaaatccat tcatcagatg tatctaaaaa ttgctcaaaa taggcattgt
     2221 caataatctt ttctggtgca tatgcaccaa aacctttaat acccacgttc atagttgaac
     2281 accttcttaa taaaattttt aataccaggt attaatttaa catagattac tttttaaact
     2341 caagtcagag gtaggaatat tgactagtta ataaaatttt aatgctttat tatcgtcaaa
     2401 tttcagtatg aaattggttt taatgcgctt aaatttgaat agccattcag ataaaatcat
     2461 ttatttaaac tgtataatat ggtcaaaata tatgagatgt tactatacaa atttctaact
     2521 ttcacttaaa ataaaatata tataattaaa gtggaggtag aatgacatga aacacctcgt
     2581 aacattcttt tgggcattat tattaatgca aatggttaac tttgtactta atagcctagt
     2641 aggtggagac agcttaaatg tagtgaatcc aattatcatg gcagtattgt tcacgatttt
     2701 cacagcaatt tttgctgcag taattaaacc gccgaaagat tcatcacaat aactatttag
     2761 tataaaatga aatatgtctt tcttattatt gaagctgccc agataaaggc ggcttttatt
     2821 tttggccatt aatgttttgt attttgaata ttttaaagct caataacctc taacccatca
     2881 cttttaaatt tagaaaaagc cttaatacaa agatgtcatt tacgagtaat ctttgtatca
     2941 aggcaattat attataatca tcactgtaaa attaaatgct tcatgttgaa tttgataatt
     3001 ttaagtgttt atttagtttt tacattaata tcaatttgtt taattgaatt tgaatcaaac
     3061 aagtttgctg tgtagctatc tttcttaaga tccacttctt ttttactacc atctttccaa
     3121 gtaatcgtgt aaacggcaga ttttgcattt cgaatcttct caggtgttac accacgttca
     3181 tcatataata aagctttaac tttatttcct aaatctaaat atgataattg gctatctttt
     3241 ggtaaattta agtaagattg attgaaagcc ataatgccat ctactgcaat tgtatatgga
     3301 acattttgca tttctttagc tttttgagtt gccggtagtt tgtccttttc tgctgcagat
     3361 acctcattac ttgtcacagt agcgccagtc gttgcgatca tgcccagtgc caaagttaca
     3421 gttataaatg attttaattt catataatct ctctcctttg tgagttaaag tatcttacaa
     3481 aatcaatata acacaaatct aaaagtttca aaaattataa gtatgta
//
LOCUS       DANBWH010000228         3523 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.236, whole genome shotgun sequence.
ACCESSION   DANBWH010000228 DANBWH010000000
VERSION     DANBWH010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.236"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..978
                     /gene="argF"
                     /locus_tag="PPY92_002433"
     CDS             <1..978
                     /gene="argF"
                     /locus_tag="PPY92_002433"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDJ2626404.1"
                     /translation="NFLTLLDFTQKEMEFLLNLSEDLKRAKYAGIEQQKMKGKNIALL
                     FEKDSTRTRCAFETAAYDQGAHVTYLGPTGSQMGKKESTKDTARVLGGMYDGIEYRGF
                     SQRVVEDLAKYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKPYNEINFTYVGDGRNN
                     VANALMQGAAIMGMTFHLVCPKELNPTDELLNRCNDIADKNGGEILITDDIDEGVKGS
                     DVIYTDVWVSMGEPDEVWEKRIKLLEPYRVTKELMKKTGNPHTIFEHCLPSFHDTETI
                     IGKQIQEKYGLTEMEVTNEVFESEQSVVFQEAENRAHTIKAVMVATLGE"
     gene            998..1927
                     /gene="arcC"
                     /locus_tag="PPY92_002434"
     CDS             998..1927
                     /gene="arcC"
                     /locus_tag="PPY92_002434"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDJ2626405.1"
                     /translation="MSKIVVALGGNALGQSPKEQLDLLKSTSKSLVSLIDKGYEIVIS
                     HGNGPQVGSINLGLNYAAEHKQGPPFPFPECGAMSQAYIGYQMQESLQNELHSMGIDK
                     EVVTLVTQVQVASDDSAFNNPTKPIGLFYTKEQADKFTKEKGYTFVEDSGRGYRRVVP
                     SPQPISIVELDSIETLITHGTLVIAAGGGGIPVIKENEVYTGVDAVIDKDKTSALLAA
                     HLQSDQLIILTAVDHVYINYGKENQRGLDEVSVDEMKKHISDGQFAKGSMLPKVEAAL
                     QFLEKNTKGSVLITSLAGLGDALDGKIGTLIKN"
     gene            complement(2027..2878)
                     /locus_tag="PPY92_002435"
     CDS             complement(2027..2878)
                     /locus_tag="PPY92_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDJ2626406.1"
                     /translation="MKRVSYSVETKYKAVEMKAAGFSKKEIMKELNIRNRTQVKTWWR
                     WYRNGESYRFLQHVGKQYTYGYRKITALINQCYTSPINHKRVQRIMQKHHLNCRVRPK
                     KTTRIGKPYYKTDNLLQRQFKASCPMEVLTTDITYLPFGHSMLYLSSIMDIYNGEIVA
                     YKIDDKQDQSLVNDTLNQIDIPESCILHSDQGSVYTSYAYYQLCEEKGIIRSMSRKGT
                     PADNAPIESFHSSLKSETFYINNELNRSNHIVIDIVEKYIKNYNNNRIQQKLGYLSSV
                     KYRELIA"
     gene            complement(3277..3519)
                     /locus_tag="PPY92_002436"
     CDS             complement(3277..3519)
                     /locus_tag="PPY92_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000931963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626407.1"
                     /translation="MLNIEVSIKDTQDIINYFIECDVNDNYYESEQRSSLGKLALELD
                     KVSNQLNNENIEDMKKIIEKVAEANRVIAKSSLRKN"
BASE COUNT         1192 a          553 c          588 g         1190 t
ORIGIN      
        1 aactttttaa ctttgttaga cttcacacaa aaagaaatgg aatttttact taatttatct
       61 gaagatctta aacgcgcaaa atatgcagga atagaacaac aaaaaatgaa aggtaaaaat
      121 atcgctctac tttttgaaaa agattcaaca cgcactcgat gtgcatttga aacagcggct
      181 tatgatcaag gtgcacatgt aacatacctt gggccaacag gttctcaaat gggtaaaaaa
      241 gagtctacca aagatactgc tcgtgtttta ggtggaatgt atgatggcat agaatatcgt
      301 ggattctcac aaagagtagt tgaggatttg gcaaaatatt ctggcgttcc cgtatggaat
      361 ggtttaacag atgaagatca tcctacacaa gtacttgctg attttttaac agctaaagaa
      421 gtattgaaaa aaccatataa tgaaattaac ttcacatatg ttggagatgg acgtaataac
      481 gttgcaaatg ccttaatgca aggagcagca atcatgggca tgactttcca tcttgtatgt
      541 cctaaagaac taaatccaac tgatgaatta ttaaatcgtt gtaatgatat agctgacaaa
      601 aacggtggtg aaatccttat aactgacgat attgatgaag gtgtcaaagg atctgatgta
      661 atttacacag atgtttgggt atctatgggt gaacctgatg aagtttggga aaaacgtatc
      721 aaattattag aaccataccg tgtaactaaa gaattaatga aaaaaacagg taatccacat
      781 acaatttttg aacattgctt accatcattc catgatactg aaacaataat cggtaaacaa
      841 attcaagaaa aatatggtct aactgaaatg gaagtaacaa atgaagtatt tgaaagtgaa
      901 caatcagttg ttttccaaga agcagaaaat agagcacata caataaaagc agtcatggta
      961 gcaacattag gagagtaaat gaaaggagga tataaatatg tctaaaatcg tcgtagcttt
     1021 gggtggtaac gctttaggac aatcgcctaa agaacaatta gatttattaa aaagtacatc
     1081 taaatctcta gtaagtttaa tcgataaagg atacgaaatt gtaattagtc atggtaatgg
     1141 tccacaagtt ggaagtatta acttaggttt aaattatgcg gcggaacaca aacaaggtcc
     1201 tccttttcct ttccctgaat gtggtgctat gagtcaagct tatattggct atcagatgca
     1261 agaaagttta caaaacgaac ttcattcaat gggcatagat aaagaagttg ttacgctagt
     1321 aacacaagtt caagttgcaa gcgatgattc tgctttcaat aaccctacta aaccaattgg
     1381 attattttat acaaaagagc aggctgataa atttacgaaa gaaaaaggtt atacttttgt
     1441 agaagattct ggacgcggct atcgtcgcgt tgtcccttcc ccacaaccta taagtatagt
     1501 tgagttagat agtatagaaa cattaatcac tcatggaaca ctagttatcg ctgccggcgg
     1561 tggtggaatt ccagtaatta aggaaaatga agtttataca ggtgttgatg cagttattga
     1621 taaagataaa acgagtgctt tattagcagc acatttacaa tctgatcaat taatcatatt
     1681 aactgctgtg gaccatgttt acattaacta tggaaaagaa aaccaaagag gtctcgatga
     1741 agtgtctgtg gatgaaatga aaaaacatat ctctgatggt caatttgcta aaggaagtat
     1801 gctcccaaaa gttgaagctg cacttcaatt tcttgaaaaa aatactaaag gcagtgtttt
     1861 gattacatct ctagcaggat taggggacgc tttagacggt aaaataggaa cattaattaa
     1921 gaattgaaat tataatactt tagttcaatt acctttataa acaaaagagc ctataatcct
     1981 agggcactga acccttaaaa cgggaactta ataaaaacac catgttctag gctattaatt
     2041 ctctgtattt tacagaggat aagtagccta gtttttgttg aattcgatta ttattatagt
     2101 ttttaatgta cttttcgaca atatctatta caatatgatt agagcgatta agctcattat
     2161 tgatgtaaaa agtttcagac tttagcgagg aatggaaact ttctatcggg gcgttatcgg
     2221 caggtgttcc ctttcgggac atacttctga taatgccttt ttcttcgcac aattgataat
     2281 aagcataaga tgtataaacg ctgccttgat cactatgtaa tatacaactc tcaggtatat
     2341 cgatttgatt taatgtatca ttaactaaac tttggtcttg tttatcatct attttatacg
     2401 ccacaatttc tccgttataa atatccatta tcgaagataa atacaacata gaatgaccaa
     2461 atggtaaata agtaatatcg gttgttaata cttccattgg acaactcgct ttaaattgtc
     2521 tttgtaataa attgtccgtt ttataatacg gtttacctat tcttgtcgtc tttttaggtc
     2581 taactcggca gtttaaatga tgcttctgca ttattctctg tactctctta tgattaattg
     2641 gtgatgtata acattgatta atcaatgctg taatcttacg ataaccgtag gtatattgtt
     2701 taccaacatg ttgtaaaaat ctataacttt ccccatttcg ataccatcgc caccaagttt
     2761 tcacttgtgt tctatttcta atatttaatt ctttcataat ttcttttttt gaaaatcctg
     2821 ctgctttcat ttcaactgct ttatactttg tttctactga ataagaaact cttttcatag
     2881 aaaaaaacac ctccgtatga ttcattttaa tatgaattca acgaaaatgt ttttatataa
     2941 ttcccactaa ttggggtcag tctacattat tgcttggctt tcgtttaatt ttatacttat
     3001 ttaatagttt aatacctact gtacagaata aacagtacag ttatgacgtg cttttcgttg
     3061 tgattctatt atatgtatag tatttgaact taaagggggg gctttgagga tttataggtt
     3121 tgacagtttt gaacttaagg caaaatatgc gataccctgt ataagcgatg tttcttcatt
     3181 tagacataag tttttcaagt tttaactcaa tatatattta aaatttcttt tgattagtag
     3241 attttattat caacttcaat catcttacta aaacactcaa ttttttctta aagaagattt
     3301 agcgataact ctatttgctt ctgctacttt ttcaattatc tttttcatat cttcaatatt
     3361 ttcattgttt aactgattac ttactttgtc caattctaaa gctaactttc cgagactgct
     3421 tctttgctca ctttcataat agttatcatt aacgtcacat tcaataaaat aatttataat
     3481 atcttgagta tccttaatac taacttctat atttaacatt tgc
//
LOCUS       DANBWH010000229         3513 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.237, whole genome shotgun sequence.
ACCESSION   DANBWH010000229 DANBWH010000000
VERSION     DANBWH010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.237"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            43..441
                     /locus_tag="PPY92_002437"
     CDS             43..441
                     /locus_tag="PPY92_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDJ2626408.1"
                     /translation="MEKSIKIMTIIGIVIQGLATVFSLLLMVLAASGVMTTDVSTTVN
                     GEVDPVDAETAAAIFTVLFLSLFIFGIISIILGAIGMFKASKNKKMSGILLIIGAVIS
                     GNIITFALWLVSGIKLLTNNKPKDEISDLS"
     gene            complement(657..1481)
                     /locus_tag="PPY92_002438"
     CDS             complement(657..1481)
                     /locus_tag="PPY92_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDJ2626409.1"
                     /translation="MKEKHIKWDKIFYGDDWVNNVVETIRTKDILQSVYLKRYLLRAM
                     MAGFIIGIITVFVLSVKATHEPDLPPGIVNMASAITFSFALVLILFTNSELLTSNFMY
                     FTVGLYYKVIKPTRVLKIFLLCFAGNILGAAILFSFMRFSNVMTPDMLNQLSAVIEHK
                     TLSTGFVSILMKAIFANFFINISLVIAMQIDDVLAKMFVMMFGVTIFAFMGYEHVVYN
                     SCLFMGGLIYQVDTLHFIPAISNIAAAFIGNYIGGGLIIGLFYAYLNDHHQFYKNN"
     gene            complement(1718..3025)
                     /gene="brnQ"
                     /locus_tag="PPY92_002439"
     CDS             complement(1718..3025)
                     /gene="brnQ"
                     /locus_tag="PPY92_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDJ2626410.1"
                     /translation="MKKQVIISGLMLFSLFFGAGNLIFPPILGHTAGQNMWIGMLGFA
                     LTGILLPFITVIVVAFYDEGVESVGNRIHPWFGFIFAVVIYMSIGAFYGIPRAANVAY
                     EIGTRHILPVHNQWTLIIFAAIFFAIVYWISLNPSKIVDNLGKLLTPLLLLMVALLSI
                     AVIFNPESALSAPKDKYITHPFISGSLEGYFTMDLVAALAFSVVIVNGYKFKGLTDRM
                     KILKYVCFSGLIAAILLGMIYFALAYVGASTAPGNFKDGTDILTYNSLRVFGSFGNIV
                     FGMTVILACLTTCIGLVNACATFTKKHVPKFSYKIFALIFSIIGFLFTTLGLEMILKI
                     AVPLLTLIYPVSIALVLISFANMFSTFRFSWAYRLATVITLIISILQILNSFNLLHGV
                     ILKWFMMLPLADIDLAWLVPFMLFAIIGFIIDVVIRRPKQATT"
BASE COUNT         1289 a          550 c          589 g         1085 t
ORIGIN      
        1 acacaaacgt aagtttgtaa ataataataa aggagtaagt caatggaaaa atcgatcaaa
       61 ataatgacaa taataggaat tgttattcag ggtttagcaa cggtatttag tttactattg
      121 atggttttag cagcatcagg tgtaatgact acagatgtgt caacaacagt taatggtgag
      181 gttgacccag ttgatgcaga aacagcagca gcaattttca ctgtattatt cttatcccta
      241 ttcatatttg gaatcatttc aattatttta ggtgcaatcg gtatgtttaa agcatctaaa
      301 aacaaaaaaa tgagtggtat attgttgatt attggagctg taataagtgg taacataatt
      361 acatttgctt tatggttagt cagtggtatt aaactactta ctaataacaa gcctaaagat
      421 gaaataagcg acttatcata aacatcgtat attgaaattt caagatttct taagtaatta
      481 aataaagtgc tctcttagaa gtagattttc agtcaataac tgcttttaag agagcatttt
      541 gtatttgata atgctcatag catcggacaa aaaccattga cgaacttaaa aaaaatacgt
      601 tagacaatgc acttctaaat atttaaggca tcgactaacg cattgttcaa atatagttaa
      661 ttatttttat aaaattgatg atgatcattc aagtaagcat agaataaacc tataatgagt
      721 ccgcctccaa tatagttacc gataaaagcc gcagcgatat tcgaaatagc tggtatgaag
      781 tgcaatgtat caacttgata aattaaacca cccataaata agcaactgtt gtaaacgacg
      841 tgttcataac ccataaaggc gaatatggta acaccgaaca tcatgacaaa catttttgcg
      901 agtacatcgt caatttgcat ggcaataact aatgaaatat tgataaagaa attggcgaag
      961 atcgctttca ttaatatact tacaaaacca gtagacaacg ttttgtgctc aataactgct
     1021 gataactgat ttaacatatc tggcgtcatt acatttgaaa aacgcatgaa actaaataaa
     1081 atagcagcac ctaaaatatt tcctgcaaag cataataaaa atattttcaa tactctagtc
     1141 ggtttaatta ctttataata caggcctaca gtaaagtaca tgaagttact ggttagtagt
     1201 tcggagtttg taaataaaat gagtactaac gcaaagctga atgtaatggc gctagccata
     1261 ttcactatac ctggtggtaa atctggttcg tgtgttgctt taactgatag cacgaagacc
     1321 gtaataatac cgataataaa tcctgccatc atcgcgcgta ataaataacg ttttaaataa
     1381 acgctttgta atatatcttt tgttcttatt gtttcgacta cgttatttac ccagtcatcc
     1441 ccataaaaaa tcttatccca tttaatatgt ttctccttca caatgacatc ctcgcttttc
     1501 atttgttaat cagagtatac tatgaatcgt cttatttgtg aataatttca caaaattaat
     1561 tttaaaaact aatatttttg aataactgat aggatttttt ggtaaatcaa ttattttaaa
     1621 ataatcaata gacagacagt atcgctgaaa atccatgaat ttcatataaa aaagcctagt
     1681 aacgaggggg caatcattac taggcagtga gcatttatta agttgtcgct tgtttcggac
     1741 gacgtataac tacatcgatg atgaaaccga taatagcaaa gagcatgaat ggtacaagcc
     1801 aagctaaatc gatatctgct aaaggtaaca tcataaacca tttcaaaata acaccgtgta
     1861 ataagttgaa actatttagt atttgtaaaa ttgaaataat caatgtaata acagttgcga
     1921 gtcgataggc ccaactgaat ctgaatgtgc taaacatgtt agcaaatgat atcagtacaa
     1981 gtgcaataga cacgggatat attaatgtca acaatggtac agcaattttt aaaatcattt
     2041 ctaaaccaag tgttgtaaat aagaacccta tgatagagaa aataagtgcg aatattttat
     2101 aagaaaactt aggtacgtgt ttcttagtaa atgtggcgca agcattgacg agtcctatac
     2161 atgttgttaa acatgcaagt atcactgtca taccaaatac tatgttgcca aatgaaccaa
     2221 atactcgtaa tgagttgtac gtcaatatat ctgtaccatc tttaaagttt cctggagctg
     2281 ttgatgcccc aacgtatgca agtgcaaagt aaatcattcc aagtaatatg gctgcaataa
     2341 gacctgaaaa gcagacatat tttaaaattt tcatgcgatc tgtgaggcct ttaaacttat
     2401 aaccattgac aatgactacg gaaaaagcta acgcagcaac aagatccatt gtaaaatagc
     2461 cttccaaact tcctgaaatg aaaggatgtg ttatatattt atccttaggt gcacttagtg
     2521 cagattcagg gttgaaaatg acagcaatac ttaatagagc gaccattaat agtaataatg
     2581 gtgttaataa tttacctaaa ttatcaacga ttttcgatgg atttaaacta atccagtaaa
     2641 cgatggcaaa aaagattgct gcgaatataa ttaaagtcca ttggttatgc acaggtaaaa
     2701 tgtgtcttgt accaatttcg tacgcgacat ttgcagcacg tggaataccg taaaatgctc
     2761 cgatagacat gtaaatcacg acagcaaaaa taaacccgaa ccatggatgt atacgattgc
     2821 ctacactttc aacaccttca tcataaaatg caacaacaat aacagtaata aaggggagta
     2881 atatgcctgt aagggcaaag cctagcatac caatccacat attttgaccc gctgtatggc
     2941 caagaatggg cgggaatatt aaatttccgg ctccaaaaaa tagtgaaaat aacatgaggc
     3001 ccgaaataat aacttgtttt ttcaatgtaa tttccttcct aaaactagat attcataata
     3061 atttaaaaaa tctgaaaaat aaaaacgttc ttactttatc tttaaatgac aatactatga
     3121 ttctattatt ttataaaaaa gattgcaata ataacgtctt gctattattg gataatgaca
     3181 attaaagtgt cgtttttatg aattaaaata ggtgaaagta agttggaaat acgttggagc
     3241 gttgtgattt ttatgagaaa ttgtatgagt gaggactgct atttataaaa ggtaaactat
     3301 ggatgagttt atggttgttc caataggtaa ataagagata gcacactgta cacataatga
     3361 tacgtggatg agtgaatatg tctgatagga aggcctagtc actcaaatga atttaagttt
     3421 attacgtgat aaatcacaaa tctctctcat gtgataggtc tcccattaaa tcatgcttat
     3481 atgaaatgtt cacatatttg ttagcttttc aag
//
LOCUS       DANBWH010000230         3483 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.238, whole genome shotgun sequence.
ACCESSION   DANBWH010000230 DANBWH010000000
VERSION     DANBWH010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.238"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..147)
                     /locus_tag="PPY92_002440"
     CDS             complement(<1..147)
                     /locus_tag="PPY92_002440"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499560.1"
                     /note="catalyzes the formation of
                     (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-
                     carboxamido)succinate from
                     5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate and
                     L-aspartate in purine biosynthesis; SAICAR synthase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDJ2626411.1"
                     /translation="MTLLYEGKAKRIFSTNQENELRVEYKDEVTAGNGAKKDTMAGKG
                     RLNNQ"
     gene            complement(151..1275)
                     /gene="purK"
                     /locus_tag="PPY92_002441"
     CDS             complement(151..1275)
                     /gene="purK"
                     /locus_tag="PPY92_002441"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDJ2626412.1"
                     /translation="MNFNKLKFGATIGIIGGGQLGKMMAQSAQKMGYKVVVLDPSEDC
                     PCRYVAHEFIQAKYDDEKALNQLGQKCDVITYEFENISAQQLKLLCEKYNIPQGYQAI
                     QLLQDRLTEKETLKSAGTKVVPFISVKESTDIDKAIETLGYPFIVKTRFGGYDGKGQV
                     LINNEKDLQEGFKLIETSECVAEKYLNIKKEVSLTVTRGNNNQITFFPLQENEHRNQI
                     LFKTIVPARIDKTAEAKEQVNKIIQSIHFIGTFTVEFFIDSNNQLYVNEIAPRPHNSG
                     HYSIEACDYSQFDTHILAVTGQSLPNSIELLKPAVMMNLLGKDLDLLENEFNEHPEWH
                     LHIYGKSGRKDSRKMGHMTVLTNDVNQTEQDMYAKFEGSN"
     gene            complement(1262..1744)
                     /gene="purE"
                     /locus_tag="PPY92_002442"
     CDS             complement(1262..1744)
                     /gene="purE"
                     /locus_tag="PPY92_002442"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDJ2626413.1"
                     /translation="MKVAVIMGSSSDWKIMQESCNMLDYFEIPYEKQVVSAHRTPKMM
                     VQFASEARERGINIIIAGAGGAAHLPGMVASLTTLPVIGVPIETKSLKGIDSLLSIVQ
                     MPGGIPVATTAIGAAGAKNAGILAARMLSIQNPSLVEKLNQYESSLIQKVEDMQNELQ
                     "
     gene            1945..2805
                     /gene="folD"
                     /locus_tag="PPY92_002443"
     CDS             1945..2805
                     /gene="folD"
                     /locus_tag="PPY92_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDJ2626414.1"
                     /translation="MVAKILDGKQIAKDYRQGLQDQVEALKEKGFTPKLSVILVGNDG
                     ASQSYVRSKKKAAEKIGMISEIVHLEETATEEEVLNELNRLNNDDSVSGILVQVPLPK
                     QVSEQKILEAINPEKDVDGFHPINIGKLYIDEQTFVPCTPLGIMEILKHADIDLEAKN
                     AVVIGRSHIVGQPVSKLLLQKNASVTILHSRSKDMASYLKDADVIVSAVGKPSLVTKD
                     VVKEGAVIIDVGNTPDENGKLKGDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLL
                     AEKMRRGIDS"
BASE COUNT         1110 a          611 c          577 g         1185 t
ORIGIN      
        1 ttgattattt aatcgcccct tacctgccat tgtgtctttc ttagccccgt ttccagcagt
       61 aacttcatct ttatattcaa ctcttaattc attttcttga tttgttgaga aaatgcgctt
      121 cgcttttcct tcatataata atgtcatgct ttaattactc ccctcaaatt tagcgtacat
      181 atcttgttca gtttggttta catcattcgt tagtacagtc atatgcccca tttttctgct
      241 atctttacgc ccagacttac cataaatatg taagtgccac tctggatgtt cattaaattc
      301 attttccaat aaatctaagt ctttacctag taagttcatc atgactgcag gctttaataa
      361 ttcaattgaa tttggtaatg attgtccggt aactgctaaa atatgagtat caaattgtga
      421 ataatcacat gcttcaattg aataatgtcc ggaattgtga ggccttggtg caatctcgtt
      481 cacatacaat tggttgttac tatctataaa aaattccact gtaaatgttc caatgaaatg
      541 aatcgattgg ataattttat taacttgctc tttcgcctca gctgttttat ctattctcgc
      601 tggaacaatt gttttgaaaa gtatttgatt tctatgctca ttttcttgta atgggaaaaa
      661 agtgatttga ttgttgtttc ctcttgtaac agtaagagat acttctttct tgatattcaa
      721 atatttttca gctacgcatt cactagtttc aattaattta aaaccttctt gtaagtcttt
      781 ttcgttgtta attaaaactt gacctttgcc atcgtagcca ccaaatctag tttttacaat
      841 aaaaggatat cctaatgttt caattgcttt gtcaatatct gtagattctt ttactgaaat
      901 gaacgggaca actttggtac cagcactttt taatgtttct ttttcagtta agcggtcttg
      961 taataactgt atagcttggt aaccttgcgg aatattgtac ttttcacata atagttttaa
     1021 ttgttgggct gaaatgtttt caaattcata agtaatcaca tcacattttt gtcctaattg
     1081 attgagtgcc ttttcatcgt catacttggc ttgtataaat tcgtgtgcaa cgtatctaca
     1141 tggacaatct tcagaaggat ccaatacaac cactttataa cccatttttt gagctgattg
     1201 tgccatcatc tttccaagct gaccaccacc aataatgcca atagtcgcac caaactttaa
     1261 tttattgaag ttcattttgc atgtcctcca ctttttgaat taacgaagat tcatactgat
     1321 ttagtttttc aactaaagaa ggattttgaa tacttaacat tcttgctgca agtatacctg
     1381 cgtttttagc acctgctgca ccaattgcag tcgttgcaac cggaatacct cctggcattt
     1441 gaacaattga taataaagaa tctataccct ttaaactttt tgtttcaatc ggcacaccaa
     1501 taactggtag cgtcgttaat gatgcaacca tacctggtaa atgtgccgca ccgccagcgc
     1561 ctgcaatgat aatgtttata cctctttctc tcgcttcaga agcaaattga accatcattt
     1621 ttggcgtacg atgtgcggat actacttgtt tttcgtacgg aatttcaaaa taatccaaca
     1681 tgttacaact ctcttgcata attttccaat cggaagaact gcccataatg actgctactt
     1741 tcactttgta caccctttca aaagtttgaa ttgtgaatta ctttagttgt atattataga
     1801 tatagcataa caagcaattt ctgctttttc aatcaaaaat cgaactttat tttgattttt
     1861 tgtttgaatt tacgtctttt gctatgtaaa ttagttttat aaactaacaa agttaggata
     1921 ttgacaatag gaggagaagt ttttatggtt gctaaaattt tagatggtaa acaaattgcc
     1981 aaagactaca gacaggggtt acaagatcaa gttgaagcgc taaaagaaaa gggttttaca
     2041 cctaaattat ccgttatatt agttggtaat gatggcgcta gtcaaagtta tgttagatca
     2101 aaaaagaaag cagctgaaaa aattggtatg atttcagaaa tcgtacattt ggaagaaaca
     2161 gctactgaag aagaagtatt aaacgaacta aatagactaa ataatgatga ttctgtaagt
     2221 ggtattttgg tacaagtacc attaccaaaa caagttagcg aacagaaaat attagaagca
     2281 atcaatcctg aaaaagatgt ggacggattt catccaataa atatagggaa attatatatc
     2341 gatgaacaaa cttttgtacc ttgcacaccg ctcggcatca tggaaatatt aaaacatgct
     2401 gatattgatt tagaagctaa aaatgcagtt gtaattggac gaagtcatat tgtcggacaa
     2461 ccagtttcta agttactact tcaaaaaaat gcatcagtaa caatcttaca ttctcgttca
     2521 aaagatatgg catcatattt aaaagatgct gatgtcattg tcagtgcagt tggtaagcct
     2581 agtttagtaa caaaagatgt ggtcaaagaa ggagcagtaa ttatcgatgt tggcaatacg
     2641 ccagatgaaa atggcaaatt aaaaggtgac gttgattatg atgcggttaa agaaattgct
     2701 ggagctatta caccagttcc tggtggcgtt ggtccattaa caattactat ggtattaaat
     2761 aatactttgc ttgcagaaaa aatgcgtcga ggtattgatt cgtaaagagc ctgagacata
     2821 aatcaatgtt ctatgctcta caaagttata atggcagtag ttgactgaac gaaaattcgc
     2881 ttgtaacaag cttttttcaa ttctagtcaa ccttgccggg gtgggacgac gaaataaatt
     2941 ttacgaaaat atcatttctg tcccactccc taataactga gttttaatga actcttttaa
     3001 cccacattaa atattatttt gcaattgcaa tgaataacaa gaaaaacctg ggacattaat
     3061 cgatcaaatg ctcccttcaa agtagacatt gaataaatga aggctttgaa gggagcattt
     3121 cactttgttc ttggctcaac aattttatat agacagtagt taattgaatg aaaataagct
     3181 tgtaacaagc atttttcaat tatagtccgg ggccccaaca aagaagctga cgaaaagtca
     3241 gcttacaata atgtgcaagt tggggatggg ccccaacaca gagaatttcg aaaagaaatt
     3301 ctacagacaa tgcaagtcag cgaggcccca acaaagaagc tgtgactatg ataaagtact
     3361 actacatagt taatcattag tggttcttta tcattttcgc ctcccttttc ttattgtttt
     3421 gatacacaaa aatttaagtt caaactgtcg aataaagtta tatttgattt caaattatcc
     3481 cta
//
LOCUS       DANBWH010000231         3480 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.239, whole genome shotgun sequence.
ACCESSION   DANBWH010000231 DANBWH010000000
VERSION     DANBWH010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.239"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            43..540
                     /locus_tag="PPY92_002444"
     CDS             43..540
                     /locus_tag="PPY92_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626415.1"
                     /translation="MQHLIKKHVLNGEFDLVRQLMSETDFMEFEEAYISSAHEVESMM
                     FYTCILDMIKYEESSEMHDLAFLLLVYPLSEYEGALDSAYYHADASIKLTDGKEVKSL
                     LQMLLLHAIPTPVISDKKAFDIAKQILKLDPNNNVARNVLKDTAKRMDNVVVDINELH
                     QRNAR"
     gene            997..2064
                     /locus_tag="PPY92_002445"
     CDS             997..2064
                     /locus_tag="PPY92_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626416.1"
                     /translation="MMMNKEATKIGFAYVGIVVGAGFSTGQEVMQFFTKYGLWAYLGV
                     IISGFILAFIGRQVAKIGTAFEATNHESTLQYVFGEKFSKVFDYILIFFLFGIAVTMI
                     AGAGATFEESYNIPTWLGALIMTLAIYITLLLDFNKIVRALGIVTPFLIVLVVLIAGV
                     YLFKGHVSLAEVNQVVPEASIWKGIWFGTIYGGLAFSVGFSTIVAIGGDTEKRTVSGA
                     GAMYGGIIYTVLLALINFALQSEYPTIKNASIPTLTLANNIHPLIATVLSVIMLAVMY
                     NTILGLMYSFAARFTEPYSKNYHIFIIIMMVAGYLLSFVGFAELINKLYTIMGYVGLF
                     IVVAVIIKYFKRKNADKKHIA"
     gene            2215..3258
                     /locus_tag="PPY92_002446"
     CDS             2215..3258
                     /locus_tag="PPY92_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDJ2626417.1"
                     /translation="MNKDNKWTMITALFITVISVLLAFHLKQHYDQITNENHANKDKI
                     NIKNKNVRIYQNLTYNRVFPNSKLDIITPVDMSSNAKLPVIFWMHGGGYIAGDKQYKN
                     PLLAKIAEQGYIVVNVNYALAPQYKYPTPLIQMNQATQFIKENKMNLPIDFNQVIIGG
                     DSAGAQLASQFTAIQTNDRLREAMKFDQSFKPSQIKGAILFGGFYNMQTVRETEFPRI
                     QLFMKSYTGEEDWEKSFKNISQMSTVKQSTKNYPPTFLSVGDSDPFESQNIEFSKKLQ
                     ELNVPVDTLFYDGTHHLHHQYQFHLNKPESIDNIKKVLLFLSRNTSSSGIQTEEKPQM
                     ENPSNELPLNPLN"
BASE COUNT         1223 a          487 c          590 g         1180 t
ORIGIN      
        1 gtaaatgttt aataaaatct ggagaaatag gaggacattg ccatgcaaca ccttataaaa
       61 aaacatgtat tgaatggcga gtttgattta gtacgacaat tgatgtccga aacagatttt
      121 atggaatttg aagaagcata tatttcaagt gcgcatgaag tagaaagtat gatgttttat
      181 acatgtattt tagatatgat taagtacgaa gaatcatctg aaatgcatga cttagcattt
      241 ttattgcttg tgtatccact aagtgaatat gaaggtgctt tggattctgc ttattatcat
      301 gcagacgctt ccataaaact tactgacggc aaagaagtta aaagtttgtt acaaatgtta
      361 ttattgcatg cgataccaac acctgttatt tcagataaga aggcttttga tatcgccaag
      421 caaattttaa aattagatcc taataataat gttgctcgta acgtcttaaa agacactgcc
      481 aaacgtatgg acaacgttgt tgttgatata aatgaattac accaacgtaa tgcacgttaa
      541 ttacatttca attatattag cttaataata gttttaacat ttggttgggt tgggacatat
      601 gttccagcct tttttaatac ttaaaaacta acgaagtata cttgtgtgca caaatggttt
      661 ttatacaaca ttttataaat ttatacattt taataaagaa catacgatat atggtttaaa
      721 ccttgttaac tgagagattt tgatatgtat tcttcgaaat ttaactaaat ttacgaaatt
      781 caagaagcac aataattaat caattttcct atacaaaagt tcgtatgact gcattataaa
      841 agcataaatt tataattttt ttaaatgtca ttgaacgtga taatgtgaat ggattgagca
      901 attttgaaaa agtgaaaaat aacctatgcg acttgcaatt aattttcagt acgttataat
      961 gcacactgtg caaaattaag gaggtctatt attcacatga tgatgaataa agaagcaaca
     1021 aaaattggat ttgcctacgt cggcattgta gtgggcgcag gattttcaac tggacaagaa
     1081 gttatgcaat ttttcactaa atatggcttg tgggcttatt taggtgttat tatatctggt
     1141 tttattttag cttttattgg gcgccaagta gcaaaaattg gtactgcctt tgaagcgaca
     1201 aaccatgaat caacattaca atacgtattc ggtgaaaagt ttagtaaagt ctttgattat
     1261 attttaatct tcttcttatt tggtatagct gtaaccatga tagctggtgc aggcgcaaca
     1321 tttgaagaaa gttataacat acctacatgg ctaggtgctt taattatgac attagcgatt
     1381 tatattacgt tgctattaga ctttaataaa atagtacgtg cacttggtat cgttacacca
     1441 tttttaattg ttttagttgt attaatcgct ggcgtttatt tatttaaagg tcatgtttca
     1501 ttagcagaag ttaaccaagt agtgcctgaa gcaagtattt ggaagggaat ctggtttggt
     1561 acaatatatg gtggattagc tttttctgta ggttttagta ccatcgtagc aatcggtggg
     1621 gatacagaaa agcgtacagt gtcaggtgca ggcgcgatgt atggtggtat tatctatact
     1681 gtattactag cattgatcaa ctttgcattg caaagtgaat atccaactat taaaaatgcc
     1741 tcaattccaa cattgacgtt agcaaataat atccatcctt taatagcaac agtgttatct
     1801 gttattatgc tggcggttat gtataatact attctaggac taatgtattc atttgcagca
     1861 cgttttacag aaccatacag taaaaattat catatcttta ttattataat gatggtagca
     1921 ggttatttat taagtttcgt aggatttgct gaattaatta ataagttata tacaattatg
     1981 ggatatgtag gcttatttat tgtagtagct gtaattatta aatatttcaa acgtaaaaat
     2041 gcggataaaa aacatattgc ttaatatcat atgatggata tccgaaactt tacaattgaa
     2101 tcactttgtt ttaaccttaa aagcaattcg tctctactct tatcgggcga attgcttttt
     2161 atatttattc agtctattaa tatgagcgtc taacaaatag agaggtacga tgtaatgaat
     2221 aaagataata aatggacgat gataactgcg ctttttataa ctgtaatcag tgtattgtta
     2281 gcatttcatc tgaaacaaca ttatgaccaa attacaaatg agaaccatgc taataaagac
     2341 aaaattaata ttaaaaataa aaatgtgcgc atttatcaaa accttacata caatagagtt
     2401 ttccctaaca gtaaattaga tattattaca cctgttgata tgtcttctaa tgccaaactg
     2461 ccagttattt tttggatgca cggtggtggt tatattgcgg gtgataagca gtataaaaac
     2521 ccattattag cgaaaattgc tgaacaaggg tacattgttg tgaatgtaaa ttatgcattg
     2581 gcgccacaat ataaatatcc cacaccatta attcaaatga atcaagcaac tcaattcatt
     2641 aaagaaaata aaatgaattt acctattgat tttaatcaag taattattgg cggtgattct
     2701 gcaggtgctc aattagctag ccaatttacg gcaatacaga cgaatgatcg cttaagagaa
     2761 gccatgaaat ttgatcagtc attcaaacca tcgcaaatta aaggtgctat actatttggt
     2821 ggtttttata atatgcaaac agttagagaa actgagtttc caagaataca gttatttatg
     2881 aaaagttata ctggcgaaga agattgggaa aagagcttta aaaatatttc acaaatgtcg
     2941 acagtaaaac aatcgacaaa aaattatcca ccaacatttt tatctgttgg agatagcgat
     3001 ccattcgaaa gtcaaaatat agaattcagt aagaaattac aagaattgaa tgtaccagta
     3061 gatactttgt tttatgatgg tacgcatcat ttacatcatc agtatcaatt tcaccttaat
     3121 aaacctgaat cgatagataa catcaaaaaa gtgttacttt tcttaagtcg taatacatct
     3181 tctagtggta ttcaaactga agagaaacca caaatggaaa atccgagtaa tgaattaccg
     3241 ttaaatcctt taaactaatg ataaacagta gtaatttatt acttaagcaa catttaagat
     3301 cttcaaattg aaaacgaaga atttaaaaca tgtggggcta atgtgtaaga gtctgagata
     3361 taataaattg ttagcggttc tttatcattt ctatctcacc ctattatgat gtgacattta
     3421 ttttacaaac taatttgttt tgaacctgaa aataactttt tacaactaaa ttgtcgaaaa
//
LOCUS       DANBWH010000232         3451 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.240, whole genome shotgun sequence.
ACCESSION   DANBWH010000232 DANBWH010000000
VERSION     DANBWH010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.240"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            58..1410
                     /gene="spa"
                     /locus_tag="PPY92_002447"
     CDS             58..1410
                     /gene="spa"
                     /locus_tag="PPY92_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal protein A"
                     /protein_id="HDJ2626418.1"
                     /translation="MKKKNIYSIRKLGVGIASVTLGTLLISGGVTPAANAAQHDEAQQ
                     NAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNNFNK
                     DQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNNFNK
                     EQQNAFYEILNMPNLNEEQRNGFIQSLKDDPSQSANLLSEAKKLNESQAPKADNKFNK
                     EQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPKEEDNKKP
                     GKEDGNKPGKEDGNKPGKEDNKKPGKEDGNKPGKEDNNKPGKEDGNKPGKEDNNKPGK
                     EDGNKPGKEDGNKPGKEDGNGVHVVKPGDTVNDIAKANGTTADKIAADNKLADKNMIK
                     PGQELVVDKKQPANHADANKAQALPETGEENPFIGTTVFGGLSLALGAALLAGRRREL
                     "
     gene            complement(1739..3331)
                     /locus_tag="PPY92_002448"
     CDS             complement(1739..3331)
                     /locus_tag="PPY92_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDJ2626419.1"
                     /translation="MTLLTVNPFDNVGLSALVAAVPIILFLLCLTVFKMKGIYAALTT
                     LVVTLIVALFVFELPARVSAGAITEGVVAGIFPIGYIVLMAVWLYKVSIKTGQFSIIQ
                     DSIASISEDQRIQLLLIGFCFNAFLEGAAGFGVPIAICAVLLIQLGFEPLKAAMLCLI
                     ANGAAGAFGAIGLPVSIIDTFNLSGGVTTLDVARYSALTLPILNFIIPFVLVFIVDGM
                     KGIKEILPVILTVSGTYTGLQLLLTIFHGPELADIIPSLATMVVLAFVCRKFKPKNIF
                     RLEASEHKIQKRTPKEIVFAWSPFVILTAFVLVWSAPFFKKLFQPGGALESLVIKLPI
                     PNTVSDLSPKGIALRLDLIGATGTAILLTVIITILITKLKWKSAGALLVEAIKELWLP
                     ILTISAILAIAKVMTYGGLTVAIGQGIAKAGAIFPLFSPVLGWIGVFMTGSVVNNNTL
                     FAPIQATVAQQISTSGSLLVAANTAGGVAAKLISPQSIAIATAAVKKVGEESALLKMT
                     LKYSIIFVAFICVWTFILTLIF"
BASE COUNT         1292 a          672 c          544 g          943 t
ORIGIN      
        1 caatacataa ttcgttatat tatgatgact ttacaaatac atacaggggg tattaatttg
       61 aaaaagaaaa acatttattc aattcgtaaa ctaggtgtag gtattgcatc tgtaacttta
      121 ggtacattac ttatatctgg tggcgtaaca cctgctgcaa atgctgcgca acacgatgaa
      181 gctcaacaaa atgcttttta tcaagtgtta aatatgccta acttaaacgc tgatcaacgt
      241 aatggtttta tccaaagcct taaagatgat ccaagccaaa gtgctaacgt tttaggtgaa
      301 gctcaaaaac ttaatgactc tcaagctcca aaagctgatg cgcaacaaaa taacttcaac
      361 aaagatcaac aaagcgcctt ctatgaaatc ttgaacatgc ctaacttaaa cgaagcgcaa
      421 cgtaacggct tcattcaaag tcttaaagac gacccaagcc aaagcactaa tgttttaggt
      481 gaagctaaaa aattaaacga atctcaagca ccgaaagctg ataacaattt caacaaagaa
      541 caacaaaatg ctttctatga aatcttgaat atgcctaact taaacgaaga acaacgcaat
      601 ggtttcatcc aaagcttaaa agatgaccca agccaaagtg ctaacctatt gtcagaagct
      661 aaaaagttaa atgaatctca agcaccgaaa gcggataaca aattcaacaa agaacaacaa
      721 aatgctttct atgaaatctt acatttacct aacttaaacg aagaacaacg taacggcttc
      781 atccaaagcc ttaaagacga tccttcagtg agcaaagaaa ttttagcaga agctaaaaag
      841 ctaaacgatg ctcaagcacc aaaagaggaa gacaacaaaa aacctggtaa agaagacggc
      901 aacaaacctg gcaaagaaga cggcaacaag cctggtaaag aagacaacaa aaaacctggt
      961 aaagaagacg gcaacaagcc tggtaaagaa gacaacaaca aacctggcaa agaagacggc
     1021 aacaagcctg gtaaagaaga caacaacaag cctggtaaag aagacggcaa caagcctggt
     1081 aaagaagacg gcaacaaacc tggtaaagaa gacggcaacg gagtacatgt cgttaaacct
     1141 ggtgatacag taaatgacat tgcaaaagca aacggcacta ctgctgacaa aattgctgca
     1201 gataacaaat tagctgataa aaacatgatc aaacctggtc aagaacttgt tgttgataag
     1261 aagcaaccag caaaccatgc agatgctaac aaagctcaag cattaccaga aactggtgaa
     1321 gaaaatccat tcatcggtac aactgtattt ggtggattat cattagcctt aggtgcagcg
     1381 ttattagctg gacgtcgtcg cgaactataa aaacaaacaa tacacaacga tagatatcat
     1441 tttatccaaa ccaattttaa cttatatacg ttgattaaca cattcttatt tgaaatgata
     1501 agaatcatct aaatgcacga gcaacatctt ttgttgctca gtgcattttt tattttactt
     1561 acttttctaa acaacttctg aaacgcctca acactttcta ctctgattac atatacgaca
     1621 tttttagaca ttaaaaaatc gaactagaca agatgctcat tgcatttcgt actagttcga
     1681 ttcatgaata attagattta aaatgtcatt tgaatccaag tgacaacatt atttatattt
     1741 agaatattaa cgttagtata aacgtccaaa cacaaataaa ggcaacaaat ataatactgt
     1801 attttaacgt catttttaat aatgcagatt cttcaccaac ttttttaaca gctgcagtcg
     1861 caatggcaat tgattgtggt gaaataagtt tcgctgctac accacctgca gtgttagctg
     1921 ccacaagtaa tgaaccgctt gttgaaattt gttgtgccac tgtcgcttga ataggtgcga
     1981 ataaagtatt gttatttaca actgaaccag tcataaacac accaatccaa cctaatactg
     2041 gagagaataa tgggaaaatt gctcccgctt tagcaatacc ttgtccaatt gctacagtca
     2101 aaccaccgta tgtcataact ttagcaatag ctaggatagc tgaaattgta aggatcggta
     2161 accataattc tttaattgct tcgaccaata aagcacctgc acttttccat tttaacttcg
     2221 taattaaaat tgtaataatt actgttaata aaatcgctgt cccagttgca ccaattaaat
     2281 cgagacgcaa cgcaattcct ttaggcgata aatcactcac agtatttgga attggcaatt
     2341 ttattactaa actttcaagt gcacctccag gttggaataa ttttttgaag aatggtgcac
     2401 tccatactaa tacaaaggca gttaaaatta cgaacggact ccaagcaaag acaatttctt
     2461 taggcgttcg tttttgaatt ttatgttcag acgcttccaa tctgaaaatg tttttcggtt
     2521 taaatttacg acaaacaaat gctaacacca ccattgttgc tagtgatgga ataatgtctg
     2581 ctagttctgg accatggaat attgttaata ataattgtaa tccagtatat gtaccactca
     2641 ctgttaaaat gacaggtaaa atttctttaa tacctttcat accatctaca atgaatacta
     2701 aaacaaatgg aataataaag tttaaaattg gaagtgttaa tgctgagtat ctcgcaacat
     2761 ctaatgttgt aacgcctcca cttaagttaa acgtatcaat aatactaact ggtaaaccaa
     2821 ttgcaccaaa ggcacccgcc gcaccattag caattaaaca taacatcgct gcttttaatg
     2881 gttcaaatcc aagttgaatt aataatactg cacaaatcgc aattggcaca ccaaatcctg
     2941 ctgcaccttc taaaaatgcg ttgaaacaaa atccaattaa taatagttgg attctttggt
     3001 cctctgaaat acttgcaata ctatcttgaa taatagaaaa ttgtcctgtt ttaatagaaa
     3061 ctttatataa ccaaactgcc attaaaacga tatatcctat tgggaaaata ccggcaacaa
     3121 cgccttctgt aatcgcacct gctgatacac gcgctggtaa ttcaaataca aataaagcca
     3181 caatcaatgt aacaaccaaa gttgtcaatg ctgcataaat gcctttcatt ttaaaaacgg
     3241 ttaagcataa taaaaataaa ataataggta ctgctgcaac taaggctgat aatccaacat
     3301 tatcgaatgg atttacagta agtagtgtca taatgactcc ctctctttat ataaaatatt
     3361 tatcattctg attaatctac aacctatttc aacttatatt ttgcgatgat cacatattta
     3421 aaatgtaaca ctcctatatg tgacaggcaa t
//
LOCUS       DANBWH010000233         3438 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.241, whole genome shotgun sequence.
ACCESSION   DANBWH010000233 DANBWH010000000
VERSION     DANBWH010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.241"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            123..1352
                     /locus_tag="PPY92_002449"
     CDS             123..1352
                     /locus_tag="PPY92_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HDJ2626420.1"
                     /translation="MSLRDEALEMHKRNQGKLEVKPNVKVTNKEELSLAYSPGVAEPC
                     KDIYEDKRKVYDYTIKGNTVAVITDGTAVLGLGNIGPEASIPVMEGKAVLFKSFAGIN
                     GVPIALNTTDTEEIIKTVKLLEPNYGGINLEDISAPRCFEIEERLKKETNIPVFHDDQ
                     HGTAIVTMAGLVNALRVVNKDIAKIKVVLNGAGAAGIAIVKLLYAYGVRNMVMCDSRG
                     AIFEGRSYGMNPTKDVVAKWTNKDKIEGSLEEVVKDADVFIGVSVANALSQDMVKSMA
                     DNPIIFAMANPNPEIIPDDAKAAGARVVGTGRSDYPNQINNVLAFPGIFRGALEVEAT
                     HINEEMKKAAVEAIADLIDSSELNEDYCIPGPFDKRVAPSVARNVAKAAMESGVARIE
                     VDPQDVYDKTMKITDLQ"
     gene            1547..2404
                     /locus_tag="PPY92_002450"
     CDS             1547..2404
                     /locus_tag="PPY92_002450"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDJ2626421.1"
                     /translation="MFKDFFNRTKKKKYLTVQDSKNNDVPAGIMTKCPKCKKIMYTKE
                     LAENLNVCFNCDHHIALTAYKRIEAISDEGSFTEFDKGMTSANPLDFPSYLEKIEKDQ
                     QKTGLKEAVVTGTAQLDGMKFGVAVMDSRFRMGSMGSVIGEKICRIIDYCTENRLPFI
                     LFSASGGARMQEGIISLMQMGKTSVSLKRHSDAGLLYISYLTHPTTGGVSASFASVGD
                     INLSEPKALIGFAGRRVIEQTINEKLPDDFQTAEFLLEHGQLDKVVHRNDMRQTLSEI
                     LKIHQEVTK"
     gene            2404..3348
                     /locus_tag="PPY92_002451"
     CDS             2404..3348
                     /locus_tag="PPY92_002451"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HDJ2626422.1"
                     /translation="MLDFEKPLFEIRNKIESLKESQDKNDVDLQEEIDMLEASLERET
                     KKIYTNLKPWDRVQIARLQERPTTLDYIPYIFDSFMELHGDRNFRDDPAMIGGIGFLN
                     GRAVTVIGQQRGKDTKDNIYRNFGMAHPEGYRKALRLMKQAEKFNRPIFTFIDTKGAY
                     PGKAAEERGQSESIATNLIEMASLKVPVIAIVIGEGGSGGALGIGIANKVLMLENSTY
                     SVISPEGAAALLWKDSNLAKIAAETMKITAHDIKQLGIIDDVISEPLGGAHKDVEQQA
                     LAIKSAFVAQLDSLESLSRDEIANDRFEKFRNIGSYIE"
BASE COUNT         1226 a          466 c          692 g         1054 t
ORIGIN      
        1 tacataatca aaaccataaa ttcttactca ttcagattta cccaatattt ttacttttat
       61 aatgtaatgc gttttatcca agttattttt taaaaataaa tattgaatta ggggctgatt
      121 tcatgtcatt aagagatgaa gcattggaaa tgcacaaacg taatcaaggt aaattagaag
      181 ttaaaccaaa tgtaaaagtt actaataaag aggaattaag tttagcatac tcacctggcg
      241 ttgctgaacc gtgtaaagat atttatgaag ataaaagaaa agtatatgat tacacaatta
      301 aaggaaatac agttgcagtt attactgatg gaacagcggt attaggttta ggtaacattg
      361 gacctgaagc aagtattcct gtaatggaag gtaaagcagt attattcaaa agcttcgctg
      421 gtatcaatgg ggtgcctatt gcgttaaata caactgatac cgaagaaatc attaaaacag
      481 ttaagttgtt agaacctaat tatggtggta ttaatttaga ggatatttcg gcaccacgtt
      541 gttttgaaat tgaagaacga ttgaaaaaag aaactaatat tccggtattc catgacgatc
      601 aacatggtac agcaattgta acaatggcag gtttggtaaa tgcattgaga gttgttaaca
      661 aagatattgc taaaataaaa gttgtactaa atggtgctgg tgcagcagga atagccattg
      721 ttaaattact atacgcgtat ggtgtaagaa atatggttat gtgtgactca agaggcgcaa
      781 tttttgaagg acgttcatat ggtatgaatc ctacgaaaga tgttgtagct aaatggacaa
      841 ataaagataa gattgaaggg tctttagaag aagtcgtaaa agacgcagat gtatttatcg
      901 gagtttctgt agctaatgcg ctgtcacaag atatggttaa gagtatggca gataatccaa
      961 ttatatttgc aatggctaat ccaaatcctg aaataatacc tgatgatgcc aaagcggcag
     1021 gtgcacgagt tgttggtaca ggacgttcag actatcctaa ccaaattaat aatgtattag
     1081 ctttccctgg tatttttaga ggtgcattag aggttgaagc tacacatata aatgaagaaa
     1141 tgaaaaaggc agctgtagaa gcgattgctg atttaatcga tagttctgaa ttaaatgaag
     1201 actactgtat cccaggaccg tttgataaac gtgtagcgcc atcagttgct cgtaatgttg
     1261 ctaaagcggc aatggaatct ggagtagcta ggattgaagt tgatccgcaa gatgtgtatg
     1321 ataaaacaat gaaaattaca gatttacaat aaaaataatt aacgtttaat taaattgctt
     1381 aaaagttagc aataaagagt cattaaaatg atgaaaatga aagtttatga taaacattca
     1441 acagtcaaac gaatataaat caaataaatt taaacccgtt tttaactggt caagttcagt
     1501 ttaaggctct aaatggttag aacagaggtt atttggaggt tttcctatgt ttaaagattt
     1561 ttttaatcga acaaagaaaa agaaatatct tacagtacaa gactctaaaa ataatgatgt
     1621 gcctgcaggt attatgacta agtgtccaaa gtgtaagaaa attatgtaca caaaagaatt
     1681 agctgaaaat ttaaatgtgt gctttaattg tgatcatcat attgctttaa ctgcgtataa
     1741 acgtatagaa gcaatttctg atgaaggatc atttacagaa ttcgataagg gaatgacctc
     1801 tgcgaatcca ttagattttc caagttattt agaaaaaatt gaaaaggacc aacaaaagac
     1861 aggtcttaaa gaagcagttg tgactggtac agcgcaacta gatggtatga aatttggcgt
     1921 tgctgtcatg gattcacgtt ttagaatggg aagtatggga tcggttatcg gtgaaaagat
     1981 atgtcgcatc attgattact gcactgagaa ccgtttacca tttattcttt tctctgcaag
     2041 tggtggtgca cgtatgcaag aaggtattat ttccttgatg caaatgggta aaaccagtgt
     2101 atctttaaaa cgtcattctg acgctggact attatatata tcatatttaa cacatccaac
     2161 tactggtggt gtatctgcaa gttttgcatc agttggtgat ataaatttaa gtgagccaaa
     2221 agcgttgata ggttttgcag gtcgtcgagt tattgaacag acaataaacg aaaaattgcc
     2281 agatgatttc caaactgcag aatttttatt agagcatgga caattggata aagttgtaca
     2341 tcgtaatgat atgcgtcaaa cattgtctga aattctaaaa atccatcaag aggtgactaa
     2401 ataatgttag attttgaaaa accacttttt gaaattcgaa ataaaattga atctttaaaa
     2461 gaatctcaag ataaaaatga tgtggattta caagaagaaa ttgacatgct tgaagcgtca
     2521 ttggaacgag aaactaaaaa aatatataca aatctaaaac catgggatcg tgtgcaaatt
     2581 gcgcgtttgc aagaaagacc tacgacccta gattatattc catatatctt tgattcgttt
     2641 atggaactac atggtgatcg taattttaga gatgatccag caatgattgg tggtattggc
     2701 tttttaaatg gtcgtgctgt tacagttatt ggacaacaac gtggaaaaga tacaaaagat
     2761 aatatttatc gaaattttgg tatggcgcat ccagaaggtt atcgaaaagc attacgttta
     2821 atgaaacaag ctgaaaaatt caatcgtcct atctttacat ttatagatac aaaaggtgca
     2881 tatcctggta aagctgctga agaacgtgga caaagtgaat ctatcgcaac aaatttgatt
     2941 gagatggctt cattaaaagt accagttatt gcgatagtca ttggtgaagg tggcagtgga
     3001 ggtgctctag gtattggtat tgccaataaa gtattgatgt tagagaatag tacttactct
     3061 gttatatctc ctgaaggtgc agcggcatta ttatggaaag acagtaattt ggctaaaatt
     3121 gcagctgaaa caatgaaaat tactgcccat gatattaagc aattaggtat tatagatgat
     3181 gtcatttctg aaccacttgg cggtgcacat aaagatgttg aacagcaagc tttagctatt
     3241 aaatcagcgt ttgttgcaca gttagattca cttgagtcat tatcacgtga tgaaattgct
     3301 aatgatcgct ttgaaaaatt cagaaatatc ggttcttata tagaataatc aacttgagca
     3361 tttttatgtt aaatcgatac tgggttttac cataaattga agtacattaa aacaataatt
     3421 taatatttag atactgaa
//
LOCUS       DANBWH010000234         3409 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.242, whole genome shotgun sequence.
ACCESSION   DANBWH010000234 DANBWH010000000
VERSION     DANBWH010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.242"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            35..1027
                     /gene="sph"
                     /locus_tag="PPY92_002452"
     CDS             35..1027
                     /gene="sph"
                     /locus_tag="PPY92_002452"
                     /EC_number="3.1.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
                     /protein_id="HDJ2626423.1"
                     /translation="MVKKTKSNSLKKVATLALANLLLVGALTDNSAKAESKKDDTDLK
                     LVSHNVYMLSTVLYPNWGQYKRADLIGQSSYIKNNDVVIFNEAFDNGASDKLLSNVKK
                     EYPYQTPVLGRSQSGWDKTEGSYSSTVAEDGGVAIVSKYPIKEKIQHVFKSGCGFDND
                     SNKGFVYTKIEKNGKNVHVIGTHTQSEDSRCGAGHDRKIRAEQMKEISDFVKKKNIPK
                     DETVYIGGDLNVNKGTPEFKDMLKNLNVNDVLYAGHNSTWDPQSNSIAKYNYPNGKPE
                     HLDYIFTDKDHKQPKQLVNEVVTEKPKPWDVYAFPYYYVYNDFSDHYPIKAYSK"
     gene            complement(1265..2281)
                     /gene="lukG"
                     /locus_tag="PPY92_002453"
     CDS             complement(1265..2281)
                     /gene="lukG"
                     /locus_tag="PPY92_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit G"
                     /protein_id="HDJ2626424.1"
                     /translation="MIKQLYKNITICSLAISTALTVFPATSYAKINSEIKAVSEKNLD
                     GDTKMYTRTATTSDSQKNITQSLQFNFLTEPNYDKETVFIKAKGTIGSGLRILDPNGY
                     WNSTLRWPGSYSVSIQNVDDNNNTNVTDFAPKNQDESREVKYTYGYKTGGDFSINRGG
                     LTGNITKESNYSETISYQQPSYRTLLDQSTSHKGVGWKVEAHLINNMGHDHTRQLTND
                     SDNRTKSEIFSLTRNGNLWAKDNFTPKDKMPVTVSEGFNPEFLAVMSHDKKDKGKSQF
                     VVHYKRSMDEFKIDWNRHGFWGYWSGENHVDKKEEKLSALYEVDWKTHDVKFVKVLND
                     NEKK"
     gene            complement(2303..3358)
                     /gene="lukH"
                     /locus_tag="PPY92_002454"
     CDS             complement(2303..3358)
                     /gene="lukH"
                     /locus_tag="PPY92_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit H"
                     /protein_id="HDJ2626425.1"
                     /translation="MKNKKRVLIASSLSCAILLLSAATTQANSAHKDSQDQNKKEHVD
                     KSQQKDKRNVTNKDKNSTVPDDIGKNGKITKRTETVYDEKTNILQNLQFDFIDDPTYD
                     KNVLLVKKQGSIHSNLKFESHKEEKNSNWLKYPSEYHVDFQVKRNRKTEILDQLPKNK
                     ISTAKVDSTFSYSSGGKFDSTKGIGRTSSNSYSKTISYNQQNYDTIASGKNNNWHVHW
                     SVIANDLKYGGEVKNRNDELLFYRNTRIATVENPELSFASKYRYPALVRSGFNPEFLT
                     YLSNEKSNEKTQFEVTYTRNQDILKNRPGIHYAPPILEKNKDGQRLIVTYEVDWKNKT
                     VKVVDKYSDDNKPYKEG"
BASE COUNT         1092 a          560 c          491 g         1266 t
ORIGIN      
        1 ttttgttgta agctatataa aaggagtgat aatgatggta aaaaaaacaa aatccaattc
       61 actaaaaaaa gttgcaacac ttgcattagc aaatttatta ttagttggtg cacttactga
      121 caatagtgcc aaagccgaat ctaagaaaga tgatactgat ttgaagttag ttagtcataa
      181 cgtttatatg ttatcgaccg ttttgtatcc aaactgggga caatataaac gcgctgattt
      241 aatcggacaa tcttcttata ttaaaaataa tgatgtcgta atattcaatg aagcatttga
      301 taatggtgca tcagacaaat tattaagtaa tgtgaaaaaa gaatatcctt accaaacacc
      361 tgtactcggt cgttctcaat caggttggga caaaactgaa ggtagctact catcaactgt
      421 tgctgaagat ggtggcgtag cgattgtaag taaatatcct attaaagaaa aaatccagca
      481 tgttttcaaa agcggttgtg gattcgataa tgatagcaac aaaggctttg tttatacaaa
      541 aatagagaaa aatggtaaga acgttcacgt tatcggtaca catacacaat ctgaagattc
      601 acgttgtggt gctggacatg atcgaaaaat tagagctgaa caaatgaaag aaatcagtga
      661 ctttgttaaa aagaaaaata tccctaaaga tgaaacggta tatataggtg gcgaccttaa
      721 tgttaataaa ggcactccag agttcaaaga tatgcttaaa aacttgaatg taaatgatgt
      781 tctatatgca ggtcataata gcacatggga ccctcaatca aattcaattg cgaaatataa
      841 ttaccctaat ggtaaaccag aacatttaga ctatatattt acagataaag atcataaaca
      901 accaaaacaa ttagtcaatg aagttgtgac tgaaaaacct aagccatggg atgtatatgc
      961 gttcccatat tactacgttt acaatgattt ttcagatcat tacccaatca aagcctatag
     1021 taaatagtgc tcaactaact aataactcgc ttcgttctaa aaggacgaag cgagttatat
     1081 tgttaaaatt tgaattgact tacattttaa taaaatcatc ttaacaactt taatttttca
     1141 ttaatacaag tctttactct acactcaaac aagattcata cactgcacat cataataaat
     1201 ctatctactc aaatataaat aaaagttacc tactacattc tatgtagcag gcaactttta
     1261 ttacttattt cttttcatta tcattaagta cttttacaaa cttcacatca tgtgtcttcc
     1321 aatcaacttc atataatgct gataattttt cttctttttt atctacatgg ttttcaccag
     1381 accaatagcc ccagaaacca tggcgattcc aatctatttt aaactcatcc attgatcttt
     1441 tataatgaac aacaaattgt gatttacctt tgtctttttt atcatgtgac ataacagcta
     1501 aaaattctgg attaaaccct tcagacacag ttacaggcat tttgtcttta ggtgtgaaat
     1561 tatctttcgc ccataaattt ccatttcgtg ttaaagaaaa gatttcactt ttagttctat
     1621 tatcactatc attagttaat tgtctcgtat ggtcatgtcc catattattt atcaaatgtg
     1681 cttctacttt ccaacctaca cctttatgtg acgtagattg atcaagtaat gtacgatatg
     1741 atggttgttg ataactaatc gtctctgaat aattactctc ttttgtaata tttccagtta
     1801 agcctccacg attaatcgaa aaatctccac ctgttttata accatacgta tatttaactt
     1861 ctcttgattc atcctgattt tttggtgcaa agtcagtcac atttgtattg ttgttgtcat
     1921 caacattttg aattgaaact gaataagatc caggccatct taatgtacta ttccaataac
     1981 catttgggtc taaaattctc aaaccactac caattgtacc ttttgcttta ataaatactg
     2041 tttctttatc ataattaggt tcagttaaga aattaaattg taagctttga gtaatatttt
     2101 tttgactatc acttgttgta gctgtacgtg tatacatttt agtatcacca tcaagattct
     2161 tctcagaaac agctttaatt tcagaattaa tttttgcata agaagttgcc ggaaatacag
     2221 ttaatgcagt agatattgct aaactacaaa ttgtgatgtt tttgtataat tgtttaatca
     2281 ttagtaatcc gccctttcaa tattatcctt ctttataagg tttattgtca tcagaatatt
     2341 tatcaacgac tttaactgtt ttatttttcc aatcaacttc ataagtgaca attaaccttt
     2401 gaccatcttt atttttttct aaaattggag gtgcataatg tattccaggt ctgtttttca
     2461 aaatatcttg atttcgtgtg tatgttactt caaattgcgt tttctcattt gacttttcat
     2521 tagataaata agttaaaaat tctggattaa agccacttct tactaatgct gggtatctat
     2581 attttgaagc aaagcttagt tcagggtttt ctacagtagc aattctcgta tttctataga
     2641 ataataattc atcatttcta tttttcactt ctccaccata cttcaagtca ttcgcaataa
     2701 ctgaccagtg tacatgccag ttattatttt taccgctggc aattgtgtca taattttgct
     2761 gattataact aatcgttttg gagtagctat ttgatgaagt tcgtccaata cctttcgttg
     2821 aatcgaattt accacctgag ctatatgaaa atgtactgtc tactttcgca gttgaaattt
     2881 tatttttcgg caattggtct aatatttcag ttttacgatt tctttttact tgaaaatcta
     2941 catggtactc acttggatac tttaaccaat ttgaattttt ttcttcttta tgagattcaa
     3001 actttaaatt tgaatgaatt gagccttgtt ttttaacaag taatacattc ttgtcataag
     3061 ttggatcatc gataaagtcg aattgtaaat tttggagtat atttgttttc tcatcatata
     3121 ctgtttcagt tcgttttgtg attttaccgt ttttcccaat atcatcaggt actgttgaat
     3181 ttttatcttt attagtaaca ttacgtttgt ctttttgttg agacttatca acatgttctt
     3241 tcttattttg atcttgcgag tctttatgag ctgaatttgc ttgagtcgtt gctgctgata
     3301 acaataaaat tgcacatgat aatgatgacg ctattaaaac acgtttttta tttttcattg
     3361 ttataacctt ctttcgtatg attgatattt gttgatatgt atcgacatg
//
LOCUS       DANBWH010000235         3384 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.243, whole genome shotgun sequence.
ACCESSION   DANBWH010000235 DANBWH010000000
VERSION     DANBWH010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.243"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(422..1243)
                     /locus_tag="PPY92_002455"
     CDS             complement(422..1243)
                     /locus_tag="PPY92_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDJ2626426.1"
                     /translation="MKFKIPENFNDLSLRDIFQQLKVPKKDLHHLNMSKDITINDKPA
                     RLMDKVHTGDDVFVPTIDEKSNYVPSYRYAQIKYEDDDMAIVMKPKGVKTHPNDLKES
                     NTLMNHVIYTIDSDYVEPIHRLDQETVGLLIVAKNPLMKKILDRMLEDNDITRIYKAN
                     VKALLPLKPQTIDMPIGKDKFHSNKRRVSPTGQRAITHILTSKMIKEAVCQLEIKLDT
                     GRTHQIRVHLAEIGHPVIGDPLYGDSTLRQLELESYKIEFVHPLTKEVISVSLDD"
     gene            1406..2518
                     /locus_tag="PPY92_002456"
     CDS             1406..2518
                     /locus_tag="PPY92_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HDJ2626427.1"
                     /translation="MEQRTRLALLKEIAEFLNEETEMYSMTQGALKYLIEGSNFTTGW
                     IFFINSVGEHELVSHVALPQSLTADHCHYIKDGSCWCVKAFNQRRLMKASNIVNCSRI
                     NLASKAFPSQNDNITHHATVPLKSGQEQFGILNVASPNTEIYSDEDLELLESVAFQLG
                     SAIKRIYLTDREKEAAKINERNRLARDLHDSVNQMLFSVKLTAHAAYGMSNESIAKQA
                     FKTIEKTSQNAVNEMRALIWQLKPVGLEQGLIHALTAYSKLMHIQLNVNVEGLIDLSN
                     EIEENIYRALQECINNVKKHADTNKMDLTLKQMNDILYIDVIDYGQGFEIDNVQIASS
                     HGINNIKQRVKLLRGKVTFHSQPTKGTQIQFTIPIK"
     gene            2540..3163
                     /locus_tag="PPY92_002457"
     CDS             2540..3163
                     /locus_tag="PPY92_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDJ2626428.1"
                     /translation="MNKVILVDDHYIVRQGLRFLLSTIENIEVLQDFADGETFLEYLK
                     EHEHPDIVLLDLVMPGMNGIEITEYIKAHYPEIKVLVLTSYVDDEHVISAINKGADGY
                     EMKDVEPQQLIETIRRVMNGEKMIHPKAQDVFETVSQKPHYTNKLSKREIEVLREMVK
                     GKTNKEIAETLFVSEKTIKTHVSHIFSKLQVSDRTQAAIYAMENKLI"
BASE COUNT         1216 a          500 c          565 g         1103 t
ORIGIN      
        1 ttatagtgtt caagctttat tttttgtcta tgtcaaattg aacttataag tccgatttga
       61 tatagagcca tgaaaaggaa aaacttttca tggagtccgt tttattatag aacctatttt
      121 ttatatgatg aattgttgta catgtattgg ccaaacaacc tgtgataaaa atcttatgat
      181 gtcatgtatt gtccatagat acacgtaacg cagttgaaaa agactacaag tcattatcca
      241 aggatgttat aaaatgaata attgctaaaa tatcaaaata taaaatccta agcatcgtaa
      301 aaaacaaaaa aaccgaacat ctgaaaaaat taataatttt ccatctatcc gatttaagtt
      361 gatacttgat ttagttacaa tgcggtattg catgtttaaa tatcatgcaa aactaatcaa
      421 attagtcatc caaagaaacg gaaatgactt ccttagtcaa gggatgcaca aactctattt
      481 tgtaactttc aagttctaat tgtcttaacg ttgaatcacc atataaagga tcaccaataa
      541 cagggtgacc aatttcagct aaatgcacac gaatttgatg agtacgtcca gtatccaact
      601 tgatttcaag ttggcacaca gcttctttta tcatttttga agttaaaatg tgtgtaattg
      661 cacgctgtcc agtaggagac acacgtcgtt tattcgaatg gaatttatct ttaccaattg
      721 gcatatcaat cgtttgtggt tttaaaggta aaagtgcctt aacatttgct ttgtatatcc
      781 gcgtaatatc attgtcttct aacatgcgat caagaatttt tttcattaaa ggatttttag
      841 caacaattaa taatcctact gtttcctggt ccagtcgatg aattggttcg acatagtcac
      901 tatcaatagt gtaaatcaca tgattcatta aagtattgct ttcttttaaa tcattagggt
      961 gagtcttaac acctttaggt ttcattacga ttgccatatc atcgtcttcg tatttaattt
     1021 gtgcataacg ataacttgga acataattac ttttttcatc gatggttgga acaaatacat
     1081 cgtcgccagt atgcacttta tccattaatc gcgcaggttt atcattaata gtaatatctt
     1141 tagacatatt taaatgatgt aaatcttttt taggtacctt aagttgttgg aaaatatctc
     1201 gtaaacttaa gtcattaaag ttttctggta ttttaaattt cataaaatcc tcctatagtc
     1261 tttaatatca tatcacaaat cagttctcct aaatataact attgagtaat caaggccatt
     1321 ttatcatgtt cacgtctaag atttaaaaat ataataactc aatgtataat agttaataca
     1381 cgtaaaaatg aattggagcg atttgatgga acaaaggacg cgactagctt tattaaaaga
     1441 gattgccgaa tttttaaacg aagaaactga aatgtatagc atgacccaag gtgcgttaaa
     1501 atatttaatt gagggcagta atttcacgac aggctggata tttttcatca atagcgtagg
     1561 tgaacatgaa cttgtatcac atgtggctct accacaatct ttgacagcag accactgtca
     1621 ctatattaaa gatgggtctt gttggtgtgt gaaagcattt aatcaacgta ggctaatgaa
     1681 agcgtcgaat atcgtcaatt gttctcgtat taacttagcc tctaaagcat ttcctagcca
     1741 aaatgacaat attacacatc atgcaacggt accacttaaa tcgggtcaag aacaattcgg
     1801 cattttaaat gtagcgtccc caaatactga aatatatagt gatgaagatt tagagttgct
     1861 agagtctgtc gcatttcaat tagggtcagc catcaaacgt atttatttaa cagaccgtga
     1921 aaaagaagca gcaaaaatta atgaacgtaa tcgtttagct agagatttac atgattcagt
     1981 aaatcaaatg ttattttctg taaaactaac agcacatgct gcgtatggca tgtcaaacga
     2041 atccatcgct aaacaagcat tcaagacgat tgagaaaacg agtcaaaatg ccgttaatga
     2101 aatgcgtgca ttgatttggc aacttaagcc agttggactt gagcaaggtt taattcatgc
     2161 tttgacagca tatagtaaat taatgcatat acaattaaat gtaaatgtag aaggtttaat
     2221 cgatttatct aatgaaatcg aagaaaacat atatagagca ttacaagagt gtattaataa
     2281 tgttaagaaa catgctgata cgaataaaat ggatcttaca ttaaaacaaa tgaacgatat
     2341 actatatatt gacgtgattg attatggtca aggatttgag atcgataatg ttcagatagc
     2401 ttcatcacac ggtattaata acataaaaca aagagttaaa ttattaagag gtaaagtgac
     2461 ctttcattct cagccaacaa agggtacaca aatacaattc acaattccta taaaatagct
     2521 tgatttaagg agataaccca tgaacaaagt aatattagta gatgaccatt atattgtgcg
     2581 acaaggattg cgatttttat tatccacgat tgaaaacata gaagttttac aagactttgc
     2641 agatggagaa acatttttag aatatttaaa agagcatgag caccctgata ttgtgctatt
     2701 agatttagtg atgcctggca tgaatggtat tgaaattacg gaatatatta aggcacatta
     2761 tccggaaatt aaagttttgg tattaacaag ttatgttgat gatgaacatg taatttcagc
     2821 aatcaataaa ggtgctgatg gttatgaaat gaaagacgtt gagcctcagc aattaattga
     2881 aactattaga cgagttatga acggtgaaaa aatgatacat cctaaggcac aagatgtatt
     2941 cgaaacagtt agccaaaaac cacactacac gaataagttg tcaaagagag aaattgaagt
     3001 gttacgtgaa atggttaaag gtaaaacaaa taaagagatt gcagaaactt tatttgtatc
     3061 tgaaaaaaca attaaaacac atgtcagtca tatatttagt aaattacaag ttagcgatcg
     3121 tacacaagca gcaatttatg caatggaaaa taagttgatt tagaatgagc ttttaaatat
     3181 ttgtcgaaaa atagacaatt tttaatctga ttttgatgaa ttttttatag cgatagggtt
     3241 tattatacag ttttctttgt aaatttgata tcattataaa agtgtaagtt ttatcaaatc
     3301 tagtgattgt gcataaaatt ttacttcatt tactttcgct tcattataaa gtaaacttaa
     3361 ctcttgttga cttgatcaaa attt
//
LOCUS       DANBWH010000236         3382 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.244, whole genome shotgun sequence.
ACCESSION   DANBWH010000236 DANBWH010000000
VERSION     DANBWH010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.244"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(267..1103)
                     /locus_tag="PPY92_002458"
     CDS             complement(267..1103)
                     /locus_tag="PPY92_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDJ2626429.1"
                     /translation="MDLASKYFNGVNWRYIDHSSGLEPMQSFAFDDTFCESVGKDISD
                     NVVRTWIHQHTVILGIHDSRLPFLKDGIDYLTNEIGYNAIVRNSGGLGVVLDQGVLNI
                     SLMFKGQTETTIDEAFTVMYLLISKMFEDENVDIDTMEIEHSYCPGKFDLSIDGKKFA
                     GISQRRVRGGIAVQIYLCVEGSGSERALMMQTFYEHALKGEVTKFKYPEIEPSCMASL
                     ETLLNKTITVQDVMFLLLYAIKDLGGVLNMTPITQEEWQRYDTYFDKMIERNKKMIDQ
                     MQ"
     gene            complement(1106..2092)
                     /gene="pta"
                     /locus_tag="PPY92_002459"
     CDS             complement(1106..2092)
                     /gene="pta"
                     /locus_tag="PPY92_002459"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDJ2626430.1"
                     /translation="MADLLNVLKDKLSGKNVKIVLPEGEDERVLTAATQLQATDYVTP
                     IVLGDETKVQSLAQKLDLDISNIELINPATSELKAELVQSFVERRKGKATEEQAQELL
                     NNVNYFGTMLVYAGKADGLVSGAAHSTGDTVRPALQIIKTKPGVSRTSGIFFMIKGDE
                     QYIFGDCAINPELDSQGLAEIAVESAKSALSFGMDPKVAMLSFSTKGSAKSDDVTKVQ
                     EAVKLAQQKAEEEKLEAIIDGEFQFDAAIVPGVAEKKAPGAKLQGDANVFVFPSLEAG
                     NIGYKIAQRLGGYDAVGPVLQGLNSPVNDLSRGCSIEDVYNLSIITAAQALQ"
     gene            2260..3012
                     /locus_tag="PPY92_002460"
     CDS             2260..3012
                     /locus_tag="PPY92_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent peroxidase"
                     /protein_id="HDJ2626431.1"
                     /translation="MSQAAETLDGWYSLHLFYAVDWASLRIVPKDERDALVTEFQSFL
                     ENTATVRSSKSGDQAIYNITGQKADLLLWFLRPEMKSLNHIENEFNKLRIADFLIPTY
                     SYVSVIELSNYLAGKSDEDPYENPHIKARLYPELPHSDYICFYPMNKRRNETYNWYML
                     TMEERQKLMYDHGMIGRKYAGKIKQFITGSVGFDDFEWGVTLFSDDVLQFKKIVYEMR
                     FDETTARYGEFGSFFVGHLINTNEFDQFFAIS"
BASE COUNT         1083 a          613 c          498 g         1188 t
ORIGIN      
        1 agatttacta ctaaagtatt agaacatttc aatagaaggt cgtttctttc atagtcatac
       61 gcattatata taccctattc tcaatctatt taatacgtaa aacatgaaat tttcttatta
      121 aatttattat ttccatcata tcattacttt taatttaatg atgttcaatt taaatattag
      181 gtcaataaca tatttatgct ttttatggat actttcaaaa ataacagccc caaacgataa
      241 cttgaaaggg gctgttaaat atttaactat tgcatttggt ctatcatttt cttgtttctt
      301 tcaatcattt tatcaaaata cgtatcgtat ctttgccatt cttcttgagt aatgggcgtc
      361 atatttaata caccgccaag atctttgatt gcatataata gtaaaaacat tacatcttga
      421 acagtaatcg ttttgttaag caatgtctct aatgaggcca tacaagatgg ttcaatttca
      481 ggatatttaa atttagtcac ttcacctttt aaagcatgtt cataaaatgt ttgcatcatc
      541 aatgcacgtt ctgaaccaga gccttcaaca caaagataaa tttgtacagc aataccgcct
      601 ctaactcttc gttgcgatat gcctgcaaat ttcttaccat cgatacttaa gtcaaatttt
      661 cctgggcaat aagaatgttc aatttccatc gtatcaatat caacattctc atcttcgaac
      721 attttgctaa ttaagaggta catcacagta aacgcttcat caatcgttgt ttctgtttgt
      781 cctttgaaca tcagcgatat atttaataca ccttgatcta gaacgacacc taagccacca
      841 gaatttctaa caatggcatt ataaccaatc tcattcgtta aataatcaat gccatctttt
      901 aaaaacggca atcttgaatc atgaatacca agaataacag tatgttgatg aatccaagta
      961 cgcacaacat tatctgatat atctttgccc acactttcgc aaaatgtatc atcgaatgcg
     1021 aaagattgca taggttctaa tccagaagaa tggtcgatat atcgccagtt gacgccatta
     1081 aaatatttac tcgctaaatc catcgttatt gtaaggcttg cgctgctgta ataattgaaa
     1141 gattgtatac atcttcaatt gagcagccac gtgataagtc atttactgga gaatttaaac
     1201 cttgtaatac tggaccaact gcatcatatc cacctaaacg ttgtgcaatt ttgtaaccaa
     1261 tattaccagc ttctaaactt gggaatacaa agacatttgc atcaccttgt aatttagcac
     1321 ctggcgcttt tttctcagca acacctggta caatcgcagc atcaaattgg aattcgccat
     1381 caatgattgc ttctaatttt tcttcttcag ctttttgttg tgctaatttg acagcttctt
     1441 gaacttttgt cacgtcgtct gatttagcag acccttttgt tgaaaagctt aacattgcaa
     1501 cttttggatc catgccaaag cttaatgctg attttgcact ttctactgca atttctgcaa
     1561 gtccttgtga atcaagttct ggattgattg cacaatcacc aaagatgtat tgttcatcac
     1621 ctttaatcat aaagaagata cctgatgttc ttgatacacc tggtttcgtt ttgatgattt
     1681 gtaaagctgg acgcacagtg tctcctgttg aatgtgctgc accactaact aaaccatctg
     1741 ctttaccagc ataaacaagc attgtaccga agtagttcac attgtttaat aattcttgtg
     1801 cttgttcttc agtcgcttta cctttacgtc gttcaacaaa tgattgaact aattcagctt
     1861 tcaattcact tgtcgcagga ttaattaatt caatattaga aatatcaaga tcaagttttt
     1921 gcgctaaaga ttgaacctta gtctcatcac ctaacacgat tggtgtaaca taatctgttg
     1981 cttgtaattg tgttgcagct gttagaacac gttcgtcctc tccttcaggt aatacgattt
     2041 taacgttttt accagaaagt ttgtctttta atacatttaa taaatcagcc ataatgtcct
     2101 cctgtaatat aaatcttatt aatcattcac ggtataatta tacgccattt tatttcataa
     2161 tgccacgtca ccaatttgta atcataattt tatcgttacc cctttgcttt atgataaaat
     2221 ttataaagaa ctgatgattt ttgaaaagga gcgataaaca tgagtcaagc agccgaaaca
     2281 ttagatggtt ggtatagtct acatttattt tatgcagttg attgggcatc attacgtata
     2341 gttccaaagg acgaacgcga tgcacttgtc actgaatttc aatcattttt agaaaataca
     2401 gcaactgtaa gatcatcaaa atctggtgat caagctattt ataatataac tggtcaaaaa
     2461 gcagatttgt tattatggtt cttacgtcct gaaatgaagt ctttaaatca tattgaaaat
     2521 gaatttaaca aattgcgcat tgctgacttc ctaatcccta catattcata tgtatcagtc
     2581 attgaattga gcaattattt agctggtaaa tctgatgaag atccttatga gaaccctcat
     2641 atcaaagcaa gattataccc agaattacca cattctgatt atatttgttt ctatccaatg
     2701 aacaaacgtc gtaatgaaac ttataactgg tacatgttaa ctatggaaga acgccaaaaa
     2761 ttaatgtatg accatggtat gattggtaga aaatatgctg gcaaaatcaa acaatttatt
     2821 actggttctg tagggtttga tgatttcgaa tggggcgtaa cattgttctc agatgacgta
     2881 ttacaattca aaaaaattgt atacgaaatg cgctttgatg aaacaacagc acgatacggt
     2941 gaattcggta gtttctttgt aggacatctt attaacacaa acgaattcga tcaattcttt
     3001 gcgatttctt aatacattgg tacgtttata aattaataaa aaaattccaa gcttatcggt
     3061 ttaagcttgg aatttttcgt ttatcttcag tatattcccg tatacataag acgtgatttg
     3121 gtaaatagtt gaaatctgta tgtttaaact tatatatatg tgctaatgta ttatcaataa
     3181 caaagtacac tttgctcata gcaaagtgta cccgagtagt cttccttggg agaactttaa
     3241 ctactatcac tacatataaa cgttaacctc aatagaaatt atacagtcgc tactctatac
     3301 aatttttgta atggttaact aatattattt taacctattt gaaatatttg aaacatattt
     3361 ttgtcgaatt tttttcaata at
//
LOCUS       DANBWH010000237         3355 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.245, whole genome shotgun sequence.
ACCESSION   DANBWH010000237 DANBWH010000000
VERSION     DANBWH010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.245"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            14..280
                     /locus_tag="PPY92_002461"
     CDS             14..280
                     /locus_tag="PPY92_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626432.1"
                     /translation="MKGSKQILLIMGIISLIVLFIFTLFIMAQYAKHYEQKSDSSNAH
                     TLNSSSAIIEQHTMSNLASLDLYAPVRNITSSRDIHPIYFMTKD"
     gene            424..732
                     /locus_tag="PPY92_002462"
     CDS             424..732
                     /locus_tag="PPY92_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626433.1"
                     /translation="MTVNIVFSIVFCISMVILGIYVAITKDFTLISYINQTTIADKHK
                     NQIAYIFTLCISLSAVFLMSSILCFEYDFIALSFLFLTIALLLIALFYVCFYKITKYP
                     "
     gene            complement(840..1073)
                     /locus_tag="PPY92_002463"
     CDS             complement(840..1073)
                     /locus_tag="PPY92_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626434.1"
                     /translation="MLRSLCKQNRILINAIKVGIEMKYKISLAYNLAIIIGSLIILCI
                     LISRGYDIYVILIPILTILASLINLICDIKKHK"
     gene            complement(1045..2246)
                     /locus_tag="PPY92_002464"
                     /pseudo
     CDS             complement(1045..2246)
                     /locus_tag="PPY92_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501256.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(2630..>3355)
                     /locus_tag="PPY92_002465"
     CDS             complement(2630..>3355)
                     /locus_tag="PPY92_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E domain-containing protein"
                     /protein_id="HDJ2626435.1"
                     /translation="VNELTEFGGEKIPQGHKDIFDPNLPTDQTEKVPGKPGIKNPDTG
                     KVIEEPVDDVIKHGPKTGTPETKTVEIPFETKREFNPKLQPGEERVKQEGQPGSKTIT
                     TPITVNPLTGEKVGEGQPTEEITKQPVDKIVEFGGEKPKDPKGPENPEKPSRPTHPSG
                     PVNPNNPGLSKDRAKPNGPVHSMDKNDKVKKSKIAKESVANQEKKRAELPKTGLESTQ
                     KGLIFSSIIGIAGLMLLARRRKN"
BASE COUNT         1104 a          589 c          446 g         1216 t
ORIGIN      
        1 aggatgtgaa ataatgaaag gctctaaaca aatacttttg attatgggca ttatatctct
       61 tattgtttta tttattttta cactattcat catggcacaa tatgcaaaac attatgaaca
      121 aaaatccgac agttccaacg cacatacttt aaactcatca tcagccataa ttgagcaaca
      181 tactatgtcg aatttagcgt ctctagattt atacgctcct gttcgtaaca ttacttctag
      241 tcgtgatatt catcccattt atttcatgac aaaagattaa aacttccttt ttcataaccc
      301 cattcattca actaataaat taaaaattga taaaaacgta ctgtttttta gaaacataca
      361 tatataagat acgcattcat tactaaaaga cagtacatta tattctatca ggaaggtgca
      421 acaatgaccg tcaacatcgt atttagtatc gtattctgca taagtatggt tatattaggc
      481 atttatgtcg caataactaa agatttcaca ctaatttctt atataaatca aacgaccatt
      541 gcagataaac acaagaacca aattgcatat atatttacgc tatgtatcag tttgagtgca
      601 gtatttttaa tgtcttcaat tctatgcttt gaatatgatt ttattgcatt gtcattctta
      661 tttttaacga tagcattatt acttatcgca ttattctacg tttgctttta taaaatcaca
      721 aaatatccat aaatgtttgc gatagtgtgt tggttagatt tattgatgcg ctgtcattaa
      781 gtaattgttt gcgaaaaaaa tacaaccttg tacgaataca aagttgcatt aatatagaat
      841 catttatgct ttttaatatc acaaattaaa ttgattaaac tagctaaaat agtcaaaatt
      901 ggaatcaaga ttacatatat atcataacct ctagatatta gtatacataa tataattagc
      961 gaccctatta taattgctaa attataagcc aaactaattt tatatttcat ttcaattccc
     1021 accttaatag cattgattaa aattctattt tgtttgcata gacttcttaa caaactttaa
     1081 cccagctaag ctaagcattg aaataattgc acctcctgat ataaatatat tacttaagct
     1141 tagtaaaggt ataattaaac ttatcagtcc taagataatg tatccgctgc gtaattcgat
     1201 gtagatgaga tactaaatac tttccccatc aaatgatttg gcgtttttat ttgaatggca
     1261 actgatcttg ttagtccctc tatagattgt ccaagtccca ctaatgttgc acctatatat
     1321 agtatcgcca cacttggaaa cacattaatt atcgataagc caatccccca aactaaaaca
     1381 ccaatactaa atttaaagat taatcgtttt tctgatagta aacccataat caatgacatt
     1441 aataaagatg ctatacctaa acatgatgta gctagtccat atacaccaac gccctctttt
     1501 aatatattgg aaataaacaa tggtaatgca acacgccaaa ggccagtatt aatcaatatg
     1561 caggcaaatt ggatgattat gataaatgga atttctttag attgtttcaa gaattcccaa
     1621 gtttcagaaa aatcttcttt tgagtgtcta tcaatcatgt tgttatttgt atattttaaa
     1681 agtgcattaa aaataaatcc taaaaatagc aatatactgc aaataaaaaa gacgccaaca
     1741 ttaccaacta gtattacaat gacaccaatt aaagcaggta aaataatatt tgagcctctt
     1801 tgcaaactat cgattaacgc attacttgtt gctaaatgct cctcatcaat aatttcagga
     1861 agaattgccc taaacgcagg atccgtatag cagttaataa tggtgatagc tgtagatatg
     1921 gttagaagcg tcagataatt taaatttgat gttattgcaa gtaaaggaat aattattata
     1981 atcaaactta gtataagatc agatagataa agtattttct ttttattatg tttatcagaa
     2041 tatgcgccac cgaaaatacc aaatataata aatggaagtg tttgactcat aaccatcatt
     2101 gataatttta aagatgattg gtttgtcaat tcaacagtaa accaaattat ttgtaacgaa
     2161 aacagcacaa agcaactccg acgtaagaaa ttaccaatca ataaatatgt aaagtttcta
     2221 tttttcaaaa cttctaagta caacatattt atcacctctc ataaaaataa ttgaatgcat
     2281 ccaccagcct tttttagacc ttcttctaaa ctctctttat ccaaagcgca attaattcta
     2341 ataaaattaa gtcagttaaa tatcaattat ttcgaaatat acatactact tgaaacacca
     2401 tacatacccc ccaaaatgac tactcagagg ttatattcta ctaattatga ttatattaaa
     2461 tatgaaaata ttatcaaaaa atcaaattta taacaaaaat acacccctta aagttaggtc
     2521 tttcaatcca acttttgggg tgtatatcat tctcatcata ttctaggttg tttttaacaa
     2581 actaaatata gtgaatgcaa atcaactatt atttaaatta tgaattattt taattctttc
     2641 ttctacgagc caataacatt aatccagcaa ttccaattat actactaaag atcaaacctt
     2701 tttgcgtgct ttctaaacct gtttttggta attctgctcg ttttttctct tgattagcta
     2761 ctgattcttt agcaatttta gattttttaa ctttatcatt tttatccatt gaatgaactg
     2821 ggccatttgg ttttgctctg tctttcgata atcctggatt gttaggattt actgggccac
     2881 ttggatgagt tggtctgctc ggcttctctg ggttttcagg tccttttgga tcttttggtt
     2941 tctctccacc gaactctaca atcttatcta ctggttgttt tgtgatctct tctgttggtt
     3001 gaccctcgcc aactttttca cctgttaatg ggttcactgt gattggtgtt gtgattgtct
     3061 tacttcctgg ttgtccttct tgtttcactc gctcttcacc aggttgtaat tttggattaa
     3121 actcacgttt tgtttcaaac ggtatctcta ctgtttttgt ttctggtgta cccgtttttg
     3181 gtccgtgttt aatcacatca tccactggct cttcgatcac ttttcctgtg tctggattct
     3241 tgattcctgg tttacctggt actttttccg tttgatctgt tggtaagttt ggatcaaaga
     3301 tatctttatg accttgcggt attttctcgc caccgaattc tgttaattca ttaac
//
LOCUS       DANBWH010000238         3336 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.246, whole genome shotgun sequence.
ACCESSION   DANBWH010000238 DANBWH010000000
VERSION     DANBWH010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.246"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            23..730
                     /gene="splA"
                     /locus_tag="PPY92_002466"
     CDS             23..730
                     /gene="splA"
                     /locus_tag="PPY92_002466"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplA"
                     /protein_id="HDJ2626436.1"
                     /translation="MNKNVMVKGLTALTILTSLGFAENISNQPHSIAKAEKNVKEITD
                     ATKAPYNSVVAFAGGTGVVVGKNTIVTNKHIAKSNDIFKNRVAAHYSSKGKGGGNYDV
                     KDIVEYPGKEDLAIVHVHETSTEGLNFNKNVSYTKFAEGAKAKDRISVIGYPKGAQTK
                     YKMFESTGTINHISGTFIEFDAYAQPGNSGSPVLNSKHELIGILYAGSGKDESEKNFG
                     VYFTPQLKEFIQNNIEK"
     gene            855..1577
                     /gene="splB"
                     /locus_tag="PPY92_002467"
     CDS             855..1577
                     /gene="splB"
                     /locus_tag="PPY92_002467"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplB"
                     /protein_id="HDJ2626437.1"
                     /translation="MNKNVVIKSLATLTILTSVTGIGTTLVEEVQQTAKAENNVTKIQ
                     DTNIFPYTGVVAFKSATGFVVGKNTILTNKHVSKNYKVGDRITAHPNSDKGNGGIYSI
                     KKIINYPGKEDVSVIQVEERAIERGPKGFNFNDNVTPFKYAAGAKAGERIKVIGYPHP
                     YKNKYVLYESTGPVMSVEGSSIVYSAHTESGNSGSPVLNSNNELVGIHFASDVKNDDN
                     RNAYGVYFTPEIKKFIAENIDK"
     gene            1635..2354
                     /gene="splC"
                     /locus_tag="PPY92_002468"
     CDS             1635..2354
                     /gene="splC"
                     /locus_tag="PPY92_002468"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplC"
                     /protein_id="HDJ2626438.1"
                     /translation="MNKNIVIKSMAALAILTSVTGINAAVVEETQQIANAEKNVTQVK
                     DTNNFPYNGVVSFKDATGFVIGKNTIITNKHVSKDYKVGDRITAHPNGDKGNGGIYKI
                     KSISDYPGDEDISVMNIEEQAVERGPKGFNFNENVQAFNFAKDAKVDDKIKVIGYPLP
                     AQNSFKQFESTGTIKRIKDNILNFDAYIEPGNSGSPVLNSNNEVIGVVYGGIGKIGSE
                     YNGAVYFTPQIKDFIQKHIEQ"
     gene            2475..3194
                     /gene="splF"
                     /locus_tag="PPY92_002469"
     CDS             2475..3194
                     /gene="splF"
                     /locus_tag="PPY92_002469"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplF"
                     /protein_id="HDJ2626439.1"
                     /translation="MNKNIIIKSIAALTILTSVTGVGTTMVEGIQQTAKAENTVKQIT
                     NTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYK
                     VTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPN
                     GNKLQMYESTGKVLSVNGNIVSSDAIIQPGSSGSPILNSKHEAIGVIYAGNKPSGEST
                     RGFAVYFSPEIKKFIADNLDK"
     gene            complement(3143..3250)
                     /locus_tag="PPY92_002470"
     CDS             complement(3143..3250)
                     /locus_tag="PPY92_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626440.1"
                     /translation="MLILLLISKNHLSMYGWMIYLSKLSAINFLISGEK"
BASE COUNT         1314 a          472 c          594 g          956 t
ORIGIN      
        1 taaatttgga aggaggtaaa atatgaataa aaatgtaatg gttaaaggtt taactgcttt
       61 aactatttta acatctcttg gtttcgctga aaatatttct aatcagcctc attcaattgc
      121 caaagcagaa aagaatgtca aagaaattac cgatgcaact aaggcaccat acaattcagt
      181 ggtagcattt gcgggtggta ctggtgtagt tgttggtaaa aatacaatcg taactaacaa
      241 acatatcgct aaaagtaatg atatttttaa aaatagagta gcagcacatt attcgagtaa
      301 aggtaaaggc ggaggaaact acgacgttaa agacattgta gaatatcctg gaaaagaaga
      361 ccttgcgata gttcatgttc atgaaacaag tacagaaggt ttgaatttta ataagaacgt
      421 tagttataca aaatttgcag aaggagcaaa agcgaaagat agaatttctg ttattggtta
      481 tccaaagggt gcacaaacaa aatataaaat gtttgaatcg acaggaacga ttaaccatat
      541 cagtggaacg tttatagaat ttgatgcgta tgcacaacca ggtaattcag gatctcctgt
      601 attgaattct aaacatgaac tgattggtat tttatatgca ggtagtggaa aagatgaatc
      661 tgaaaagaat ttcggtgttt atttcacacc acaattaaaa gaatttattc aaaataatat
      721 tgaaaaataa gtatcatcaa ttcattcgtg aagttgattt tttaaagagt gattaagaaa
      781 acggttacat aaccaactaa atatactgaa ttatgtatct agatatcaaa ataattaaaa
      841 aagaggaact taaaatgaac aaaaacgtag tcatcaagag tttagcaact ttaacaattt
      901 taacatctgt aacaggtatt ggaacaacat tggttgagga agttcaacaa actgccaaag
      961 cagaaaataa tgtcacaaaa attcaagata ctaatatttt tccatatact ggtgtagttg
     1021 cttttaaaag tgcaactgga tttgtagttg gaaagaatac tattttaaca aataaacatg
     1081 tgtcgaaaaa ttacaaagtg ggcgatcgta ttactgcaca tccaaatagt gataaaggta
     1141 atggtggtat ttattcgatt aaaaagatta ttaattatcc aggtaaagaa gatgtatcag
     1201 tcattcaagt tgaagagcgt gcaatagaac gtggaccaaa aggctttaat tttaatgata
     1261 atgtaacgcc attcaaatat gcggcagggg ctaaagctgg tgagcgaatt aaagtgattg
     1321 gttatccaca cccatacaaa aataaatatg ttttatatga gtcaactggc cctgtgatgt
     1381 cagtagaagg tagcagtatt gtatattcag cgcatactga aagcggaaac tctggatcac
     1441 ctgtattaaa cagcaacaac gaattagttg gtattcattt tgcttctgat gtaaaaaatg
     1501 atgataacag aaatgcatat ggcgtctact ttacaccaga aattaaaaaa ttcattgcag
     1561 aaaacataga taaataaaca aattgacttt aaacgagcgt tgcaacatat ctcgaattgt
     1621 aaaggagctt gaaaatgaat aaaaatatag tcattaaaag catggcagca ttagccattc
     1681 taacctcagt aactggaata aatgctgcag tcgttgaaga gacacaacaa atagcaaatg
     1741 cagagaagaa tgttacgcaa gttaaagata caaataattt tccatataat ggcgtcgttt
     1801 catttaagga tgcgacaggt tttgtaattg gaaaaaatac aattatcacc aataaacatg
     1861 tatcaaaaga ttataaagtt ggcgatagaa ttactgccca tccaaacggt gacaaaggaa
     1921 atggtggtat atataaaatt aaaagcattt ctgattatcc gggtgatgaa gacatctctg
     1981 tcatgaatat tgaagaacaa gcagttgaac gtggaccaaa aggctttaat tttaatgaaa
     2041 atgtccaagc attcaatttt gcgaaagatg ctaaagttga tgacaaaatt aaagttattg
     2101 gttacccatt acctgctcaa aatagtttta aacagtttga atctacagga actataaaaa
     2161 gaatcaaaga caatatttta aattttgatg catacattga acccgggaat tcaggatcac
     2221 cagttctaaa ttctaacaat gaggtcatag gtgtggtgta tggcggtatt ggaaaaattg
     2281 gttctgaata taatggtgcc gtatacttta cgcctcaaat caaagatttt attcaaaagc
     2341 acattgaaca ataaacaaat ttaaatatac accatgagca tgtgttcaat aattttaatg
     2401 aaaaacatcg gtcgaatata acataaaaaa acgtctatat caaaagcatc atgaataaac
     2461 agaggagcac aaaaatgaat aaaaatataa tcatcaaaag tattgcagca ttgacgattt
     2521 taacatcagt gactggcgtc ggcacaacaa tggttgaagg tattcaacaa acagccaaag
     2581 ccgaaaatac tgttaaacaa attacaaata caaatgttgc accatacagt ggtgttacat
     2641 ggatgggcgc tggaacagga tttgtagttg gaaatcatac aatcattacc aataaacatg
     2701 ttacctatca catgaaagtc ggtgatgaaa tcaaagcaca tcctaatggt ttttataata
     2761 acggtggtgg actttataaa gttactaaga ttgtagatta tcctggtaaa gaagatattg
     2821 cggttgtaca agttgaagaa aaatcaacac aaccaaaagg tagaaaattc aaagatttca
     2881 ctagtaaatt taatatagca tcagaagcta aagaaaatga acctatatca gtcattggtt
     2941 atccaaatcc taatggaaat aaactacaaa tgtatgaatc aactggtaaa gtattatcag
     3001 tgaatgggaa tatagtgtca tcggatgcaa ttattcagcc tggtagctct ggttcaccta
     3061 tattaaatag taaacacgaa gctattggtg taatctatgc cggtaataag ccatcaggtg
     3121 aaagcacaag aggatttgct gtttatttct ctcctgaaat taagaaattt attgcagata
     3181 atttagataa ataaatcatc catccataca ttgataaatg atttttagaa attaacaaca
     3241 aaatcaacaa ttttaaacat ctctgtgatt ctatttattc gaaatgattt aaaaaataaa
     3301 acttcaaaaa cctaacctta tatttatacg aatact
//
LOCUS       DANBWH010000239         3318 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.247, whole genome shotgun sequence.
ACCESSION   DANBWH010000239 DANBWH010000000
VERSION     DANBWH010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.247"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..1246)
                     /locus_tag="PPY92_002471"
     CDS             complement(<1..1246)
                     /locus_tag="PPY92_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDJ2626441.1"
                     /translation="MKRLSTTLKVRLISNFLQLIITTAFIPFIALYLTDMLSQSIVGI
                     YLVGLVVLKFPLSIISGYLIEIFPKKLLVLIYQATMVIMLVFMGVFGSHQLWQIIGFC
                     VAYAIFTIVWGLQFPVMDTLIMDAITEDVEHYIYKISYWMTNLSVAIGALLGGLMYGY
                     SMLLLFLIAACIFLIVLFILYIWLPQDRNQVKQSDDKRHASRYQKLQIMNIFRSYKLV
                     LKDRNYMLLISGFSIIMMGEFSISSYIAIRLKDQFETISIGSYDITGAKMLAILLMIN
                     TVVVILLTYSISKVVLKIDFKKALITGLLIYIVGYSGLTYLNQFGLLVVFMIIATVGE
                     IIYSPIVSEQRFKIIPKAKRGTYSAVNALGIHFSETLARLGIVLGVFLTSLQMGLYMF
                     IVLTIGASMLVAGVFGGQKQVNT"
     gene            complement(1632..2657)
                     /locus_tag="PPY92_002472"
     CDS             complement(1632..2657)
                     /locus_tag="PPY92_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HDJ2626442.1"
                     /translation="MKIVALFPEAVEGQENQLLNTKKAIGLKTFLEERGHEFIILADN
                     GEDLDKHLPDMDVIISAPFYPAYMTRERIEKAPNLKLAITAGVGSDHVDLAAASEHNI
                     GVVEVTGSNTVSVAEHAVMDLLILLRNYEEGHRQSVEGEWNLSQVGNHAHELQHKTIG
                     IFGFGRIGQLVAERLAPFNVTLQHYDPINQQDHKLSKFVSFDELVSSSDAITIHAPLT
                     PETDNLFDKDVLSRMKKHSYLVNTARGKIVNRDALVEALASEHLQGYAGDVWYPQPAP
                     ADHPWRTMPRNAMTVHYSGMTLEAQKRIEDGVKDILERFFNHEPFQDKDIIVASGRIA
                     SKSYTAK"
     gene            complement(2831..3190)
                     /locus_tag="PPY92_002473"
     CDS             complement(2831..3190)
                     /locus_tag="PPY92_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HDJ2626443.1"
                     /translation="MNTIDTHTKEQQFSNLVRSYRKEYVGKGPNSIRVSFKDNWAIAH
                     MTGVLSKVESFYLNDKRNESMLHYTRTEKIKQMYKEIDVNEMESLVGAKFVKLFTDID
                     LNDDEVISIFVFDKSIE"
BASE COUNT         1132 a          644 c          438 g         1104 t
ORIGIN      
        1 ttgtattcac ttgtttttgt cccccgaata caccagcaac aagcatgcta gcaccaattg
       61 ttaaaacgat aaacatatac agtcccattt gtaatgacgt taagaaaaca cccaacacaa
      121 tccctaacct agctagtgtt tctgaaaaat gaatacctaa tgcattaact gcactatatg
      181 ttcctctttt agctttagga ataattttaa agcgttgttc tgaaactata ggcgaataaa
      241 taatctcacc tacagtcgca attatcataa aaacaactaa taagccaaac tgattaagat
      301 aggttagacc actatagcca acaatataaa tcagcaaacc agtgattaaa gcttttttaa
      361 aatctatttt caatacaact ttcgagattg aatacgtgag taaaatgacg acgaccgtat
      421 taatcattag caagattgct aacatcttag cacctgtaat atcatatgaa cctatactta
      481 ttgtttcaaa ctgatccttt agtctaatag caatatatga ggagattgaa aattcaccca
      541 tcatgatgat actgaacccc gaaatcaata acatataatt acggtctttc aaaactaatt
      601 tataactgcg aaatatattc attatttgta atttttgata acgacttgca tgcctcttgt
      661 catcactttg ctttacttga tttcggtctt gaggtaacca aatatataaa ataaagagta
      721 caattaaaaa tatacaagct gctattaaga aaagtagtaa catactgtag ccatacatca
      781 agccacctaa caatgcccca atagctaccg ataagttcgt catccaatag ctaatcttgt
      841 aaatataatg ttccacgtct tcggtaattg catccataat taatgtgtcc ataactggaa
      901 attgtaatcc ccaaacgatt gtaaatatgg catatgcaac acaaaaacca ataatttgcc
      961 acaattgatg tgacccaaat acgcccatga acacaagcat tatcaccatc gtcgcttgat
     1021 aaataagtac tagcaacttt ttcggaaaaa tctcaataag gtaaccagat ataatggaca
     1081 atggaaattt tagaaccact aaaccaacaa gatatatacc gacaattgat tgacttaaca
     1141 tatctgttaa atatagtgct ataaacggta taaatgctgt cgtaataatt agctgtaaaa
     1201 aattgctaat caatcgtact ttcaaagtcg tacttaagcg tttcattaat aactacaccc
     1261 ctatccacat atatacacac taccatttat ataaaatcta ttattagatt atacgttgaa
     1321 acaaagaatt tttagttata caaagttatt taacaaacaa atgcatcgat tttacaatgc
     1381 atcttgcata taatgcctca aaaaaattag cactatatga ctatatatta ttccaaatga
     1441 atgaaattta tcaataattg cgtaagtgaa cacatatgtg cgtaaatgtt aaatcattgt
     1501 cctaacatag ccgtgactat acttagatgt aatcataaaa aaactactgc catctttaca
     1561 acagcagtag tgcttatatg attcatttat ataaaattga aagcgttaat cgctagccca
     1621 gcatccttat tctatttagc tgtataactt ttactagcaa tacgaccact tgcaacaata
     1681 atatctttat cttggaaagg ttcatgattg aagaaacgct ctaaaatatc tttaactcca
     1741 tcttcaatgc gtttttgtgc ttctaaagtc atacctgaat agtgaaccgt catagcattt
     1801 ctaggcattg ttctccatgg atgatcagca ggtgcaggtt gtggatacca aacatcacca
     1861 gcatatcctt gtaaatgctc ggatgctaac gcttcaacta acgcatcgcg atttacaatt
     1921 ttaccacgtg cagtattcac taaataactg tgttttttca tacgacttaa aacatcttta
     1981 tcaaataagt tatcagtttc tggtgttaat ggtgcatgaa ttgtaatcgc atcacttgat
     2041 gaaacaagtt catcaaagct tacaaattta gacaatttat ggtcttgttg attgattgga
     2101 tcatagtgtt gtaatgttac attaaatggc gctaatcttt cagcaacaag ttgtccaatt
     2161 cgaccaaatc caaaaatacc aattgttttg tgttgtaatt catgcgcatg attacctact
     2221 tgagacaagt tccattcacc ttctactgat tgacgatgac cttcttcata gtttctaaga
     2281 agtattaata aatccataac cgcatgttct gccacactaa ctgtattact tcctgtaact
     2341 tcaacgacac caatattgtg ttcacttgct gccgctaaat ctacatggtc agatcctaca
     2401 cctgctgtaa ttgctaattt caagttcggt gctttttcaa tacgttcacg agtcatatat
     2461 gcaggataaa atggcgcact aataatcaca tccatatctg gtaaatgttt atctaagtct
     2521 tcaccattat ctgctaatat aatgaactca tgtcctcttt cctctaaaaa tgtttttaat
     2581 cctattgctt ttttagtatt aagtaattga ttttcttgac cttctactgc ttctggaaat
     2641 aatgctacga ttttcatatc catctctcct aaatgataaa ttttgacttc cttaaagtac
     2701 gtgttgcatg ttgcttgttt taaaaatatg acaaaaaaaa cggcaccgtt tgacatacca
     2761 cccccttgaa ttattgttaa attcaaagcg aagttagcaa acagcaccgt gtaccttaca
     2821 ccagcaacac ttattctatt gacttatcga aaacaaatat tgaaatgact tcatcatcat
     2881 tcaaatcaat atctgtaaat aattttacaa acttagcgcc tacaagactt tccatctcat
     2941 ttacatctat ttctttatac atctgtttaa tcttctctgt gcgtgtataa tggagcattg
     3001 attcattgcg tttgtcgttt aggtaaaaac tctcaacttt actcaaaaca cctgtcatat
     3061 gtgcaatcgc ccaattatct ttaaacgaca ctcgaatact attgggtcct ttacccacgt
     3121 attctttacg ataagatctt actagattcg agaattgttg ttctttagta tgcgtatcta
     3181 tagtattcac cttctttaaa ataagaagtt cattaattgt tcacattttc attatacacc
     3241 ttattaacac tttttcaatg ataacgatta ttattattac gaaatgtata ttttcctagt
     3301 taaaaggata taacaaat
//
LOCUS       DANBWH010000240         3316 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.248, whole genome shotgun sequence.
ACCESSION   DANBWH010000240 DANBWH010000000
VERSION     DANBWH010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.248"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            61..1443
                     /locus_tag="PPY92_002474"
     CDS             61..1443
                     /locus_tag="PPY92_002474"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDJ2626444.1"
                     /translation="MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDN
                     DIKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIVKELQMDESILRTIDVTLEHYIN
                     QLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQIL
                     DSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENF
                     TDVSGIYKANPNIIKDPELIEEITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKN
                     TNRPQDKGTFIVHDREINAKNVISGISCDKDFTVINIKKYLMNRQVGFTRKILGVLED
                     NNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGE
                     GMNKVIGTANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHG
                     VALKNLCDKN"
     gene            complement(1497..2072)
                     /locus_tag="PPY92_002475"
     CDS             complement(1497..2072)
                     /locus_tag="PPY92_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626445.1"
                     /translation="MNAKEQLVDNLMKTSSQLFKFHGEVAMQLFLNDELKLPSIVEIC
                     VERKRLSDIVKVIPQSYALLYIDKQDQAIAKEDLSLSKIAKVYVQYDDTTIMSIFVYD
                     VVNDEWIFRLDPNIRIPKSNIYFHSLNWDVDYIKPEIVLMYDLMQHHQYHHYSNYKRV
                     IDALSYYQFFILKFVVGEQRIKDAIQRTINN"
     gene            2403..3257
                     /locus_tag="PPY92_002476"
     CDS             2403..3257
                     /locus_tag="PPY92_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626446.1"
                     /translation="MSFYVVLIIIIVALIGILVLNQRYSNSKIDTEVYARKQLIKKNK
                     ALSAENAELRSQMLSSNNDVGHHAYKNAKRELRKILDSYLENGKLKYYDIIVTSNLAT
                     KHPFFEYARSFDFIIVSDIGLINVDVKSWGEKTFYHFDVPDKHDTEMSNSNIEKVVGH
                     YISQQYHDQFNSSRKSIYTFTETVQPNRVIYDFYDYDPYQLAANNAKALKDHIEQNFN
                     FKVQSTGVIYFSDGTVNIIQGSEERDKYVDTVSTKSSLRRIISEAIELSKHPLNKEQV
                     DQITAIFK"
BASE COUNT         1220 a          477 c          516 g         1103 t
ORIGIN      
        1 cattcttact tacaaaactg aatacaattt tctgattttt tagaaaggtt ggcatgcact
       61 atgaaagttt ccaaattcgg tggtagctcc gtctccaatg ctactcaaat taaaaaggtt
      121 ttaaatattg ttaattctga tccagagcga aagattgtta tcgtttctgc tccaggtaaa
      181 agacatgata atgatattaa aacaactgat ttgttaatca gattatatga aaaggtcatt
      241 aatcatcttg attatcatga taaaaaaaga gaaattattc agcgttatga tgatattgta
      301 aaagaattgc aaatggatga aagtatttta cggacgatag atgtgacttt ggaacattat
      361 attaatcaat taaaaaatga accaaagaga ctattagatg cattactttc ttgtggtgaa
      421 gattttaatg cgcaattgat agctttatat aataatagtc aaggtatacc aacaaaatat
      481 atatcaccga aggaagcggg tatttttgtt actgacgtcc cacaagaagc acaaatatta
      541 gatagtgctt actctgaaat taaaaaatta agagattatg atgaaaaact aattatccca
      601 ggattttttg gcgtatctca tgaaggatat atagttacat ttccacgtgg cggatcagat
      661 ataactggcg ccatcatatc aagtggtgtt agagccacaa tttatgaaaa tttcaccgat
      721 gtgtctggta tttataaagc taatccaaat atcatcaaag atcctgaatt aatcgaagaa
      781 atcacttatc gagaaatgcg tgaattatct tatgctggtt ttagtgtttt ccatgatgaa
      841 gccttacaac cactttataa agatcgcatc cctgtggtga ttaaaaatac gaaccgccct
      901 caagataagg gtacatttat agtgcatgat agagaaataa atgcgaaaaa tgtgattagc
      961 ggtataagtt gtgataaaga ttttactgtc attaatatca aaaagtattt aatgaataga
     1021 caagtcggct ttactagaaa gatattaggt gtcttagagg ataataatat ttcatttgat
     1081 catatgcctt caggtattga tactatcagt atcgttatgc gttcaaaaca aattcaaaat
     1141 aaagaaacaa aagtattaaa tgaaattcgt caaaaatgtg atgttgatga gctaagtatt
     1201 gagcatgatt tagcaatatt aatgattgtc ggcgaaggta tgaataaagt catcggtact
     1261 gcaaataaaa ttacacatgc cttagctgaa tcaaaaatta acttaaaaat gattaaccaa
     1321 ggtgcttcgg aaatctcgat gatgtttgga attcatgtag atgatgctga aaaagctgta
     1381 ttatctacat atgagttttg ctatcacggg gttgcattaa aaaacttgtg tgataaaaac
     1441 taatataaaa gcaattcgta acacatgtat tcaactgcgt tatgaattgc tttttcttaa
     1501 ttatttattg ttctctggat tgcatcctta atacgttgct cacctactac aaattttaaa
     1561 ataaaaaatt gatagtagct taatgcatct atgactcgtt tataattgga ataatgatga
     1621 tactgatggt gttgcattag atcatacatt agaacgatct ccggtttaat ataatccaca
     1681 tcccaattta aactatggaa gtatatgtta ctcttaggta tacgtatatt cggatccaat
     1741 ctaaaaatcc attcatcgtt tactacatca taaacgaaaa tactcattat tgttgtatca
     1801 tcatattgca cataaacttt tgcaattttt gaaagtgata aatcttcttt agctattgct
     1861 tgatcttgct tatctatgta tagtaacgca tatgattgcg gaataacttt cacaatatca
     1921 cttaaacgct tacgttccac gcatatttca acaatagaag gtaattttaa ttcatcattt
     1981 aagaaaagct gcatggcaac ttcaccgtga aatttaaata attgcgatga tgttttcatt
     2041 aaattgtcca ctaattgttc tttagcattc atttcattca ctctcaaata cacctcctaa
     2101 gcaagcgctt tcacaaatat cattatacat tttaaaatta atgaattcaa tcttcaaaat
     2161 actatgagtg acctaattct gtcatttaaa tgaccgttat aacataaatt cgacataaac
     2221 ttgaattcac gaatctttca cgtcatattc attgaacatc aatatctgtc agtttataat
     2281 ttgattataa caatgatgaa ctaaaatcaa ctaataaaaa tgtgggaaat tgaataacaa
     2341 ttaatcttta ttaattatta ttgtatacaa agttaaagaa gtcaaaaagg attggtgaaa
     2401 aaatgtcttt ttatgttgta cttatcatta tcattgtagc attaattggt atattagttt
     2461 taaatcaaag gtatagtaat agtaaaatag ataccgaagt ttatgcaaga aagcaactta
     2521 ttaaaaagaa taaagcatta agtgcagaaa atgctgaatt aagaagtcaa atgctgagtt
     2581 ctaataatga tgttggacac catgcataca aaaatgctaa aagagaatta cgcaagattt
     2641 tagatagcta tctcgaaaat ggtaaattaa aatattacga tattatcgtt acaagcaatt
     2701 tagctactaa acatccgttt ttcgagtatg cacgttcatt tgattttatc attgtttcag
     2761 atatcggttt aataaacgtt gatgtaaaaa gttggggtga aaaaacattt tatcactttg
     2821 atgtacctga taagcatgat acagaaatga gcaatagtaa tattgaaaaa gttgtcggtc
     2881 attacattag ccagcaatat catgatcagt ttaattcatc aagaaagtct atctatactt
     2941 ttacagaaac agttcaacca aatcgtgtta tttatgattt ttatgattat gatccatatc
     3001 aattagcagc aaataatgct aaagcattaa aggatcatat tgaacaaaac tttaacttta
     3061 aagtccaaag taccggtgtt atttacttta gtgatggtac agttaatatc attcaaggtt
     3121 ctgaagaaag agataaatat gttgataccg tttcaacaaa atcttcatta cgacgtatta
     3181 ttagcgaagc catagagcta tctaaacacc ctttaaataa agaacaggtt gatcaaatca
     3241 cagctatatt caagtaaaat caaaggcata ataatccatt acgctcgata cgcactatgg
     3301 ttattatgcc tttaaa
//
LOCUS       DANBWH010000241         3244 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.249, whole genome shotgun sequence.
ACCESSION   DANBWH010000241 DANBWH010000000
VERSION     DANBWH010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.249"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            133..1308
                     /locus_tag="PPY92_002477"
     CDS             133..1308
                     /locus_tag="PPY92_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diglucosyl diacylglycerol synthase"
                     /protein_id="HDJ2626447.1"
                     /translation="MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHD
                     LFMEAHPILTSICKKWYINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIK
                     EKPDLILLTFPTPVMSVLTEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQ
                     DFIDVGIDPSTVKVTGIPIDNKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGF
                     DTMITDILAKSANAQVVMICGKSKELKRSLIAKFKSNENVLILGYTKHMNEWMASSQL
                     MITKPGGITITEGFARCIPMIFLNPAPGQELENALYFEEKGFGKIADTPEEAIKIVAS
                     LTNGNEQLTNMISTMEQDKIKYATQTICRDLLDLIGHSSQPQEIYGKVPLYARFFVK"
     gene            1286..2476
                     /locus_tag="PPY92_002478"
     CDS             1286..2476
                     /locus_tag="PPY92_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDJ2626448.1"
                     /translation="MQDSSLNNYANHKNFILMLIILFLMEFARGMYILSYINFLPTVT
                     SIAVAITSLAFSIHFIADASTNFVIGFLLKKFGTKIVLTTGFILAFTSLFLVIWFPAS
                     PFVIIFSAMMLGIAVSPIWVIMLSSVEEDKRGKQMGYVYFSWLLGLLVGMVFMNLLIK
                     VHPTRFAFMMSLVVLIAWILYYFVDVKLTNYNTRPVKAQLRQIVDVTKRHLLLFPGIL
                     LQGAAIAALVPILPTYATKVINVSTIEYTVAIIIGGIGCAVSMLFLSKLIDNRSRNFM
                     YGVILSGFILYMILIFTLSMIVNIHIVWIIALAIGLMYGILLPAWNTFMARFIKSDEQ
                     EETWGVFNSIQGFGSMIGPLFGGLITQFTNDLNNTFYFSALIFLVLAVFYGSYFIANR
                     EKAK"
     gene            complement(2589..3098)
                     /locus_tag="PPY92_002479"
     CDS             complement(2589..3098)
                     /locus_tag="PPY92_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcG family protein"
                     /protein_id="HDJ2626449.1"
                     /translation="MILGLALIPSKSFQEAVDSYRKRYDKQYSRIKPHVTIKAPFEIE
                     DGDLDSVIEQVRARINGIPAVEVHATKASSFKPTNNVIYFKVAKTDDLEELFNRFNGE
                     DFYGEAEHVFVPHFTIAQGLSSQEFEDIFGQVALAGVDHKEIIDELTLLRFDDDEDKW
                     KVIETFKLA"
BASE COUNT         1076 a          518 c          508 g         1142 t
ORIGIN      
        1 tattttgtat caattcatct attgattaaa attgctataa atgtaacgac tttgcgtctt
       61 tatttctttt ataatgccgt aaaataagtt acacttaaat aaaattttca aactaacgga
      121 gggtggctat taatggttac tcaaaataaa aagatattga ttattactgg ctcattcggt
      181 aacggtcata tgcaagttac acagagtatc gttaatcaac ttaatgatat gaatctagac
      241 catttaagcg tcattgagca cgatttattt atggaagctc atccaatttt gacttctatt
      301 tgtaaaaaat ggtatatcaa tagctttaaa tattttagaa atatgtacaa agggttttat
      361 tacagccgcc cagataaact agacaaatgt ttttacaaat actatggact taataagtta
      421 attaatttat tgataaaaga aaagccagat ttaatattat taacgtttcc tacaccagtt
      481 atgtcggtac taactgagca atttaacatt aatattccag ttgctacagt gatgacagac
      541 tatcgcttac ataaaaactg gattacgccg tattcaacaa gatattatgt ggcaacaaaa
      601 gaaacgaaac aagacttcat agacgtaggt attgatcctt caacagttaa agtgacaggt
      661 attcctattg ataacaaatt tgaaacgcct attaatcaaa agcagtggtt aatagacaac
      721 aacttagatc cagataagca aactatttta atgtcagctg gtgcatttgg tgtatctaaa
      781 ggttttgaca cgatgattac tgatatatta gcgaaaagtg caaatgcaca agtagttatg
      841 atttgtggta agagcaaaga gctaaagcgt tctttaatag ctaagtttaa atctaacgag
      901 aatgtattga ttctaggtta taccaaacac atgaatgaat ggatggcatc aagtcaactt
      961 atgattacga aacctggtgg tatcacaata actgaaggtt tcgcccgttg tattccaatg
     1021 attttcctaa atcctgcacc tggtcaagag cttgaaaatg ccctttactt tgaagaaaaa
     1081 ggttttggta aaatcgctga tactccagaa gaagcaataa aaattgtagc aagtttaacg
     1141 aacggtaatg agcaattaac taacatgatt agtacaatgg aacaagataa aattaagtat
     1201 gctacacaga caatatgtcg agacttactt gatttaatcg gtcattcatc acaaccacaa
     1261 gagatttatg gaaaggttcc tttatatgca agattcttcg ttaaataatt acgctaatca
     1321 taaaaatttc attttaatgc ttattatctt atttttaatg gaatttgcga gaggcatgta
     1381 tatcctaagc tatattaatt tcttgccaac agttacatcc attgctgtag caataacgtc
     1441 tctagcattt tcaatacatt ttattgctga tgcatctaca aactttgtta taggattttt
     1501 actgaaaaaa ttcggcacaa aaatcgtttt aacgactgga tttattttag catttacaag
     1561 tttattttta gttatttggt ttccagcatc accttttgta atcatattta gtgctatgat
     1621 gctcggtatt gcagttagtc ctatttgggt cattatgtta tccagtgttg aagaagataa
     1681 acgtggcaaa caaatgggct atgtttattt ttcatggttg ttaggcctat tggtaggtat
     1741 ggtttttatg aatctactta tcaaagtgca cccaacacgc tttgccttca tgatgtcttt
     1801 agtcgtttta attgcatgga tattatatta ttttgtcgat gtaaagttaa caaattataa
     1861 tactcgtcca gttaaagctc aattgagaca aattgttgat gtgacaaagc gccatttatt
     1921 gttgttccct ggtattcttt tacaaggtgc cgcaattgct gccctagttc ctatattacc
     1981 aacatatgct actaaggtta ttaacgtaag cacaattgaa tatactgttg caatcattat
     2041 tggtggtatc ggctgtgcag tttcgatgct atttttatcg aaactaatag ataaccgcag
     2101 cagaaacttt atgtatggtg ttattctaag cggatttatt ttatacatga tattaatttt
     2161 tactctatct atgattgtta atattcacat cgtgtggatt atcgctttag ctatcggtct
     2221 aatgtatggc atcttattac cagcatggaa tacctttatg gcacgattta tcaaatcaga
     2281 tgaacaagaa gaaacttggg gtgtttttaa tagtatccaa ggattcggct caatgatagg
     2341 gccattattt ggcggattaa ttacacaatt taccaacgat ttaaacaata cattttattt
     2401 ctcggcatta atctttttag tcttagctgt attttatgga agctacttta tagcaaatag
     2461 agaaaaagct aagtaaggtt aaaacgtatt ttaaaataaa gaaaatggtc atttttatta
     2521 cattcaataa aaatgaccat tttcttatta attcaaacat actacgatta acaatactat
     2581 tatgttactt aagctaattt aaacgtttca ataactttcc atttatcttc gtcatcgtca
     2641 aaacgtaaca aagttaattc atcgataatt tctttatggt ctaccccagc taatgctact
     2701 tgaccaaaaa tatcttcgaa ttcttggcta gatagtcctt gtgctattgt aaagtgtggc
     2761 acaaaaacat gttcagcttc tccatagaaa tcttctccat taaagcgatt aaacaattct
     2821 tctaagtcgt ccgtcttcgc aactttaaag taaatcacat tgttcgttgg tttgaagcta
     2881 gaagcttttg tagcatgaac ttctactgct ggtataccat taatacgagc tctaacctgt
     2941 tcaatgacag aatctaaatc accatcttca atttcaaatg gcgctttaat tgtcacatgt
     3001 ggtttaattc gtgaatactg tttatcatat cttttacggt aagaatccac cgcttcttga
     3061 aatgactttg atggaattaa tgctaatcct aaaatcatct gaattcctcc tttgtttaac
     3121 ctattgctat tatatcaaat ttctttgaaa accgttacat taaaaataat tcattataca
     3181 tcttttgcaa tttcgacaaa tttaaattaa atcaaacagt aaaatctttt ataaattata
     3241 ttat
//
LOCUS       DANBWH010000242         3168 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.250, whole genome shotgun sequence.
ACCESSION   DANBWH010000242 DANBWH010000000
VERSION     DANBWH010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.250"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..116)
                     /gene="rpmG"
                     /locus_tag="PPY92_002480"
     CDS             complement(<1..116)
                     /gene="rpmG"
                     /locus_tag="PPY92_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDJ2626450.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERVEMKKFCSR"
     gene            complement(229..2304)
                     /locus_tag="PPY92_002481"
     CDS             complement(229..2304)
                     /locus_tag="PPY92_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDJ2626451.1"
                     /translation="MLKRLKEKSNDEIVQNTINKRINFIFGVIVFIFAVLVLRLGYLQ
                     IAQGSHYKQIIKNDENITVNESVPRGRILDRNGKVLVDNASKMAITYTRGRKTTQSEM
                     LDTAEKLSKLIKMDTKKITERDKKDFWIQLHPKKAKAMMTKEQAMLADGSIKQDQYDK
                     QLLSKIGKSQLDELSSKDLQVLAIFREMNAGTVLDPQMIKNEDVSEKEYAAVSQQLSK
                     LPGVNTSMDWDRKYPYGDTLRGIFGDVSTPAEGIPKELTEHYLSKGYSRNDRVGKSYL
                     EYQYEDVLRGKKKEMKYTTDKSGKVTSSEVLNPGARGQDLKLTIDIDLQKEVEALLDK
                     QIKKLRSQGAKDMDNAMMVVQNPKNGDILALAGKQINKSGKMTDYDIGTFTSQFAVGS
                     SVKGGTLLAGYQNKAIKVGETMVDEPLHFQGGLTKRSYFNKNGHVSINDKQALMHSSN
                     VYMFKTALKLAGDPYYSGMALPSDISSPAQKLRRGLNQVGLGVKTGIDLPNETRGQIE
                     PLTNNPGNYLDLSIGQYDTYTPLQLSQYVSTIANDGYRIQPHIGLTIHESTNKDEVGP
                     LKKKINGTVLNKVNNTEKEIKQIQEGFKMAFNDKDGTGYVSFKDTVVPTAGKTGTAEV
                     FQNGEPRVNSTYIGYAPIDDPKLAFSIVYTNQPVPPPWLTGGDLGRDVINYYFKQLGK
                     DDKNKDKDK"
     gene            complement(2425..3024)
                     /locus_tag="PPY92_002482"
     CDS             complement(2425..3024)
                     /locus_tag="PPY92_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDJ2626452.1"
                     /translation="MAFELPKLPYAFDALEPHFDKETMEIHHDRHHNTYVTKLNAAVE
                     GTDLESKSIEEIVANLDSVPANIQTAVRNNGGGHLNHSLFWELLSPNSEEKGTVVEKI
                     KEQWGSLEEFKKEFADKAAARFGSGWAWLVVNNGQLEIVTTPNQDNPLTEGKTPILGL
                     DVWEHAYYLKYQNKRPDYIGAFWNVVNWEKVDELYNATK"
BASE COUNT          909 a          600 c          445 g         1214 t
ORIGIN      
        1 tcacgtgaac agaatttctt catttcaaca cgttctggat tatttctttt gttcttagtt
       61 gtaatgtagt ttctgtcacc acattccgta caagctaaag ttacgtttac gcgcatggtt
      121 attcctcctt atcatttcta aatacgactt tattattata ccaacatcta taatatttgc
      181 aaagttaaaa ttttacaacc atgcgctaca caatcgtcag gttaaatttt atttgtcttt
      241 gtctttattt ttatcatctt tacctaactg cttaaagtag tagttaatta catctctacc
      301 taagtctcca cctgttaacc atggtggtgg tacaggctga tttgtatata caattgaaaa
      361 cgctaatttt ggatcatcaa ttggcgcgta tcctatataa gtagagttaa ctcttggctc
      421 tccgttttgg aacacttcag cggtacccgt tttaccagca gtaggtacta ctgtatcttt
      481 aaaactaaca tatccagtac catctttatc attaaatgcc attttgaatc cttcttgaat
      541 ttgtttgatt tccttttcag tattattaac cttgttcaag acagtgccat taattttctt
      601 cttgagtgga ccaacctcat ctttattagt tgattcatga atcgttaatc caatgtgtgg
      661 ctgtattcta taaccatcat tcgctatagt tgaaacatat tgtgataatt gtaatggtgt
      721 ataggtatca tattgaccaa ttgataaatc tagataatta cctggattat ttgttaatgg
      781 ttcgatttga cctcttgttt catttggtaa atctatccct gttttcacac ctaagcctac
      841 ttgatttaat cctcttctaa gcttttgggc aggtgaactt atatctgaag gtaaagccat
      901 accagaataa taagggtctc ccgctaattt taatgctgtt ttaaacatat atacgtttga
      961 tgaatgcatc aaagcttgct tatcattaat agatacatgc ccgtttttat tgaagtatga
     1021 tcgttttgtc aaaccacctt ggaaatgtaa tggttcatcg accattgttt ctccaacttt
     1081 gatagcttta ttctgataac cagctaataa tgttccacct tttacagaag atccaaccgc
     1141 aaattgagaa gtaaacgtac caatgtcata atcagtcatt ttaccactct tattaatctg
     1201 ctttccggca agcgcaagaa tgtctccatt tttaggattt tgtacaacca tcatagcatt
     1261 atccatatct tttgcacctt ggctgcgaag cttcttaatt tgtttatcta ataatgcttc
     1321 tacttctttt tgaagatcta tatcgatcgt taatttcaaa tcttgaccgc gagcgccagg
     1381 atttaacact tctgaagatg taactttacc agatttgtcc gttgtgtatt tcatttcttt
     1441 cttcttacca cgcaatacat cttcatattg atattctagg taagattttc caacacgatc
     1501 attgcgtgaa tatcctttgg ataagtaatg ttctgtcaat tcttttggaa taccttcagc
     1561 aggtgtcgat acatctccga atatacctct taaagtatcg ccatatggat attttctatc
     1621 ccaatccata gacgtgttaa cacctggtaa tttggaaagt tgctgagaaa ctgctgcata
     1681 ctctttttca ctgacatctt cattttttat catttgtgga tctaaaactg ttcctgcatt
     1741 catctctcga aaaatagcta aaacttgtaa atctttagaa gacaattcat ctaattgtga
     1801 ttttccgatt ttcgataaca gttgtttatc atattgatct tgtttaatac ttccatctgc
     1861 taacatagct tgttcttttg tcatcattgc ttttgctttt ttaggatgca actgaatcca
     1921 gaaatctttc ttatcacgtt ctgtaatttt cttagtatcc atcttgatta gctttgataa
     1981 cttttcagcc gtatccaaca tttccgattg tgttgttttt cgacccctag tatatgtaat
     2041 agccatttta gaagcattat caactaaaac tttcccattt ctgtctaaaa tacgacctct
     2101 tggcacagac tcattcactg taatgttttc atcatttttt ataatttgtt tataatgtga
     2161 gccttgtgcg atttgtaaat aacctaaacg tagtactagt actgcaaaaa taaatacaat
     2221 cacaccaaat ataaagttaa ttctcttgtt aattgtattt tgaacgattt catcatttga
     2281 tttttctttt agtcttttta acaaaactac ctacctctat tcaaagtctt tcactttaaa
     2341 tcatatatga atttagaaat tatttctatc tttttgacaa aaaaataacg gtctcattta
     2401 agagaccgaa caagtaatca tactttattt tgttgcatta tataattcgt caactttttc
     2461 ccagttaact acattccaaa atgcgccaat gtagtcaggg cgtttgtttt gatattttag
     2521 gtaataagcg tgttcccata cgtctaaacc taaaataggt gttttaccct cagttaatgg
     2581 attatcttgg tttggtgtag tcacaatttc taactggcca ttgtttacga ctaaccaagc
     2641 ccaacctgaa ccaaagcgtg cagctgcttt gtcagcaaat tcttttttaa attcttctaa
     2701 agaaccccat tgttctttaa ttttttctac tacagtacct ttttcttctg agtttggtga
     2761 aagtaactcc cagaataatg aatggtttaa atgtccaccg ccattattac gtacagcagt
     2821 ttggatgtta gctggtacac tgtctaaatt agcaacaatt tcttcaatag atttagattc
     2881 taaatctgta ccttctactg cagcatttaa tttcgtaaca tacgtgttat gatgtctgtc
     2941 atggtgaatt tccatagttt ctttgtcaaa atgtggttct aatgcatcaa atgcgtatgg
     3001 taattttggt aattcaaaag ccataaataa tcatcctcct aaaatgtctg taagtaaata
     3061 ataacaagca gtgactggtt caacaaagaa tttgcttaaa ttctactact tattattttc
     3121 tctactcatt taatataact caaatcaaaa ataattaaac attttgta
//
LOCUS       DANBWH010000243         3121 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.251, whole genome shotgun sequence.
ACCESSION   DANBWH010000243 DANBWH010000000
VERSION     DANBWH010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.251"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            70..1545
                     /locus_tag="PPY92_002483"
     CDS             70..1545
                     /locus_tag="PPY92_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDJ2626453.1"
                     /translation="MKFKRLATIFSAVLVLSGCGSMHSSGKDLNISLPLKTKSIAPYE
                     TDVPVKIGAAESLFKTNDQGKIEKALVKSYHQPNDTTLDIELKDNIKFQNGQKLTAEK
                     VKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTNSAYPELVSELANPFMAIYYTDA
                     KSDVNQTPVGTGPYQIKDYKQSRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNL
                     ESQKDDLITDVPVNKVHDIENNQNLKVSKESGFRTSLLMYNHTNKKMTKSVREALDHI
                     IDRQGIADHIYQGYAKPATSPFNDKIPYIKEPKLTKQNIEQAKTLLAKDGYTKEHPLK
                     IKLITYDGRPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLKDRSAWDATMYSFGTI
                     PRGDTGYFFNQAYKKDGAINKGDYNNSNVDDLINQLNHTVDVKERHNISNDIIKLSSR
                     DVPNSYIAYNDQIVAANSKVKNYKVTPEGIYLIDYRTTIER"
     gene            complement(1859..3001)
                     /locus_tag="PPY92_002484"
     CDS             complement(1859..3001)
                     /locus_tag="PPY92_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HDJ2626454.1"
                     /translation="MSSASTQSTKTSDIHNESINKQMEAKAHETAQNADLKTEARSLF
                     DNATKSIGRLAGNDESLNLNLKDMFSEVFKPHTKNEADEIFIAGTAKTTPAICDISEE
                     WGKPWLFSRVFIAFTVTFIGLWVMAAIFNNTNAIPGLIFIGALTVPLSGLFFFYESNA
                     FKNISIFEVIIMFFIGGVFSLLSTMVLYRFVVFSDQFERFGSLTFFDAFLVGLVEETG
                     KALIIVYFVNKLKTNKILNGLLIGAAIGAGFAVFESAGYILNFALGENVPLLDIVFTR
                     AWTAIGGHLVWSAIVGAAIVIAKEQHGFEFKDIFDKRFLIFFLSAVGLHGIWDTSLTV
                     LGSDTLKIFILIVIVWILVFILMGAGLKQVNLLQKEFKEQQKKVDE"
BASE COUNT         1147 a          537 c          461 g          976 t
ORIGIN      
        1 aacaaaataa agttttaaaa tattaattgt tgggttttac atagtaatta gaaagagagt
       61 gttaatgaaa tgaagtttaa aagactagca actatatttt cagcagtatt agtgttatca
      121 ggatgtggct caatgcattc atctggcaaa gacttaaata tttcgttacc gttaaaaaca
      181 aagtctattg caccttatga aacagatgta ccagttaaga ttggtgctgc agaatcatta
      241 tttaaaacaa atgatcaagg aaaaatagaa aaagcattag tgaaatcgta tcatcaacca
      301 aatgatacaa cgttagatat agaattaaaa gataatatta aatttcaaaa cggtcaaaaa
      361 ttgactgcag aaaaagtgaa atctagcctt gaaaatagca tgaaaaaaag cgacttggtc
      421 aaatattcat taccaatatc atcaattacc gctaaaggtc aaaaactgac aattaaaacc
      481 aactccgctt accctgaact tgtatctgaa ttagctaatc cttttatggc aatttattat
      541 acagatgcta aatcagatgt taatcaaact cctgttggta caggccctta ccaaataaaa
      601 gattataagc aatctcgaaa aatatcattg tcgaatttta aggactattg gcaaggtaaa
      661 ccgaaacttg atcatattac tgtgacgtac caagaagacg gcaataatcg cgtcagaaat
      721 ttagaatctc aaaaagatga tttaataact gatgtcccag ttaataaagt tcatgacata
      781 gaaaataatc aaaatttaaa agtgtcaaaa gaatctggat ttagaacttc tttacttatg
      841 tataatcata ctaataaaaa aatgactaaa tccgttcgtg aagcattaga tcatatcatt
      901 gatagacaag gtattgcaga tcatatttat caaggttatg cgaaacctgc aacgagtcca
      961 tttaatgata aaattccata tattaaagaa cctaagttaa ccaaacaaaa tatcgaacaa
     1021 gcaaaaacgt tattagctaa agatggttat acaaaagaac accctttaaa aattaagtta
     1081 attacgtatg acggtcgtcc agagctatcg aaaattgcgc aagtattaca atcagatgct
     1141 aaaaaagcaa atattgaaat cgacattaaa agtgttgatg acatagaagg ttacttaaaa
     1201 gaccgttctg catgggatgc aacgatgtat agtttcggaa caatccctcg cggtgataca
     1261 ggttatttct ttaatcaagc atataaaaaa gatggtgcta ttaataaagg agactacaat
     1321 aatagcaacg tcgatgattt aattaatcaa ttgaatcata ctgtagatgt taaggaacgt
     1381 cacaatattt ctaatgatat tattaaatta tcaagtcgag atgtaccaaa cagttatatt
     1441 gcttacaacg atcaaatagt cgctgctaac tctaaggtta aaaattataa agtaacacct
     1501 gaaggtatct atttaatcga ttatcgaaca acgattgaac gataagggag tgtgggacat
     1561 tgatttatgt ctcagccctg cttcaactat aaaaaccacg ccgaagtcat ccaatatgat
     1621 tatggcgtgg tttaatttgc ttatatgatt gtattaattt ctgtttgatg aatttcaata
     1681 aaatgattat tacatacttt caatgaatca tgttcatttt atataaatct aattgtcact
     1741 atttgtttaa cgtgaaacac ttttttaact ccaaaaagta ttcctattcc actcttttaa
     1801 agacaaatta taaaaatagc ttatgtcaca aacatatgag caacataagc tttaattatt
     1861 attcgtctac ttttttctgt tgttctttaa attctttctg cagtaaattc acttgtttta
     1921 aacctgcccc cattaaaatg aatacaagta tccacacaat aacgattaaa ataaatattt
     1981 tcaacgtatc actgccaagt acagttaaag atgtatccca aatgccatgt aaaccaacgg
     2041 ctgataaaaa gaatattaaa aagcgtttat caaaaatatc tttgaattca aagccatgct
     2101 gttctttcgc aataactatt gcagcaccaa caatcgctga ccaaactaaa tgaccaccaa
     2161 tcgcagtcca cgcacgtgtg aagacaatat ctaataatgg gacattttcc cctaaagcga
     2221 aattcaaaat ataacctgct gattcaaaaa ctgcgaaccc tgcaccaata gcagcaccga
     2281 ttaataatcc attcaaaatc ttatttgttt tcaatttatt gacgaaataa acaataatga
     2341 gtgcttttcc agtttcttca actaatccta ctaaaaatgc atcgaaaaat gttaaagaac
     2401 caaacctttc gaattgatca ctaaaaacga caaatctata taataccatc gtacttagta
     2461 atgaaaatac gccgccaata aagaacatga taataacttc aaaaatgcta atatttttaa
     2521 acgcatttga ttcataaaag aagaacaaac ccgataatgg tactgttaaa gcccctataa
     2581 aaatgagacc cggaatcgcg ttagtgttat taaaaatcgc tgccatgacc cataatccaa
     2641 taaatgttac tgtgaaagcg atgaatactc gagaaaagag ccatggcttc ccccattctt
     2701 ctgatatgtc acaaattgct ggcgtagttt tagcagtacc cgctataaat atttcatctg
     2761 cttcgttttt agtatgcggc ttaaatactt cagaaaacat atcttttaaa ttaagattta
     2821 agctttcatc attgccggct agtctaccga ttgatttggt tgcattatca aataaacttc
     2881 ttgcttcggt ttttaaatct gcattttgcg ctgtttcatg cgctttggct tccatttgtt
     2941 tattgataga ttcattatgt atgtcggacg tcttagttga ttgtgtactt gctgatgaca
     3001 tcccctcttc acctgtatta ggtgcaactg attgcgtttg tgttgtctct tctttttctg
     3061 tatttcttcg atgattaaga ccttccatag acttacctac tttcactttt cataataaaa
     3121 c
//
LOCUS       DANBWH010000244         3107 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.252, whole genome shotgun sequence.
ACCESSION   DANBWH010000244 DANBWH010000000
VERSION     DANBWH010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.252"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(47..376)
                     /locus_tag="PPY92_002485"
     CDS             complement(47..376)
                     /locus_tag="PPY92_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626455.1"
                     /translation="MINETKYMRQQITNLIQIIDTIKYNTLMTDWNIQTHIYKFNQIV
                     TNELNKFKGFETSYIINENQVSYYEIITLLNKRPLRQVDYGNKIQYLNFYHTELSNAL
                     FAIKFAH"
     gene            425..1162
                     /gene="sdpB"
                     /locus_tag="PPY92_002486"
     CDS             425..1162
                     /gene="sdpB"
                     /locus_tag="PPY92_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpB"
                     /protein_id="HDJ2626456.1"
                     /translation="MENEKKKYTFKDIAWRDLLIIPIIGVIFYALTIAAYIGPILFGY
                     YEIDSNLIFVATITQALAYTIGIGAFYLFHLKVMPSRLRAGFAYIKKYWLRLSITYII
                     ALILIYTYEYMTQFLPKHLQYSETANELELNKMFEVPAFLPVAFLLIVIVGPIVEEIV
                     FRHILIGELGKKFNFIAMSIVSVFLFAFIHVTDAKSPFEFGPYLILSIILVFTYLKSG
                     RNLGSTIALHIANNFVSFVISVISIYA"
     gene            complement(1187..1879)
                     /locus_tag="PPY92_002487"
     CDS             complement(1187..1879)
                     /locus_tag="PPY92_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDJ2626457.1"
                     /translation="MNKAERQNLIITAIQQNKKMTALELAKYCNVSKRTILRDIDDLE
                     NQGVKIYAHYGKNGGYQIQQAQSKIALNLSETQLSALFLVLNESQSYSTLPYKSEINA
                     IIKQCLSLPQTRLRKLLKRMDFYIKFDDTQHMTLPMLFSDILIYCTERNVMLVDHRVD
                     DNIKAENVIFIGLLCKHGHWHAVIYDIAQDKTAELEIENIIDISYSFGKTIQTRDISI
                     DNYHQFLNPIDS"
     gene            2264..3061
                     /locus_tag="PPY92_002488"
     CDS             2264..3061
                     /locus_tag="PPY92_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HDJ2626458.1"
                     /translation="MTDKTLQQIDKLICSWLKQIDNVIPQLIMEMTTETKRHRFDLVT
                     NVDKQIQQQFQQFLATHFPEHQLLAEEKSNEMITNEINHLWIMDPIDGTANLVKQQED
                     YCIILAYFYEGKPMLSYVYDYPHKKLYKAIRGEGAFCNGIKMEEPPSLKLEDAIISFN
                     AQVMNLDTVQDLFDASFSYRLVGACGLDSMRVAKGQFGAHINTNPKPWDIAAQFLFAE
                     LLNLKMTTLDGKAIDHLKGAPFIISNKACHETVLKILNANGGYQKYR"
BASE COUNT         1066 a          415 c          523 g         1103 t
ORIGIN      
        1 aattttaata aataacttgt ttaaatgaca ttttatgaaa aataggttaa tgggcaaatt
       61 taattgcaaa taatgcgtta gatagttctg tatgataaaa attaagatat tgaattttgt
      121 taccatagtc gacttgtcgg aggggacgtt tattaagtag tgttataatt tcataatagg
      181 aaacttgatt ttcgtttatt atatatgatg tttcaaagcc tttgaactta ttcaattcat
      241 tagtaactat ttggttaaat ttataaatat gcgtttgaat gttccaatct gtcattaaag
      301 tattatattt aatcgtatca atgatttgaa tcaaattagt aatttgttgt ctcatatact
      361 tagtttcgtt tatcatgtag aagtacctcc aaaaatcaat tattaaacag gaaaggatat
      421 aacagtggaa aatgaaaaaa agaaatacac gtttaaagat atagcatgga gagatttgct
      481 tataattcca attataggtg ttatatttta tgcactcaca attgcagctt acataggacc
      541 aattttgttt ggttattatg aaatcgatag taatctgata tttgtagcga ctatcacaca
      601 agctttagca tatacgattg ggattggcgc attttattta tttcatttaa aagtgatgcc
      661 atcaaggctt cgagctggtt ttgcgtatat caaaaaatat tggcttagat tgtcaataac
      721 gtatattatt gctctcatct taatttatac atatgaatat atgacacagt ttctacctaa
      781 acatttgcaa tatagtgaaa cagccaatga acttgagcta aataaaatgt ttgaagtgcc
      841 tgcattttta cctgtagcat tcttattaat tgtgattgta ggtccaatag ttgaagaaat
      901 cgtatttaga catattttaa ttggagaatt aggtaagaag tttaatttta tagctatgag
      961 tattgtttcg gtgttcttat ttgcatttat tcatgtgact gatgcaaagt cgccgtttga
     1021 gtttggtcca tatctcatac tatccatcat attagttttc acttatttaa aatctggaag
     1081 aaacctaggt tcaacaattg cattacatat agcaaataat tttgtttctt tcgttatatc
     1141 agtaataagt atttatgcgt agataaaacc ccatcgactc catagtttag gaatcgatgg
     1201 ggtttaaaaa ttgatgatag ttatcaatgg atatgtctct ggtttgaatc gtcttaccga
     1261 atgaatacga aatatctata atattttcaa tttcgagttc ggcagttttg tcttgagcaa
     1321 tgtcataaat gactgcatgc caatgtccat gtttacacaa aaggccaata aatataacgt
     1381 tttcagcttt aatattatca tcaaccctat gatctactaa catcacattt cgttctgtac
     1441 aataaattaa aatgtcggaa aacagcattg ggagtgtcat atgttgtgtg tcatcaaatt
     1501 taatataaaa gtccatgcgt ttaagcaatt ttcttaagcg tgtttgtgga agacttaaac
     1561 attgttttat aattgcgttg atttcgcttt tatatggtaa tgtcgagtac gactgacttt
     1621 cattaagcac taaaaataag gccgataatt gtgtttcaga taagtttaat gcaattttag
     1681 attgtgcttg ttgtatttgg taaccaccat ttttcccata atgcgcataa attttaacac
     1741 cttgattttc taaatcatca atatctctta aaattgtgcg tttggatacg ttgcaatatt
     1801 tagctaattc taaagcggtc atttttttat tttgttgaat tgcagtaatt attaaatttt
     1861 gcctttcagc tttattcatg acagccacct cctaaaagat gatatattac acaaagtata
     1921 acataagagt gacaatgagg acattttcaa tatcttttaa aaaaaatcgt aaaaattttt
     1981 tggaaaacgc ttacatttta aaaaagattg tgctatgata ttacttgtaa cagggggagc
     2041 gattaaacaa aggggtagag ctatataaca gatagctttt aatcgttcaa gttacatcac
     2101 aacatcttca atatttatta cttactttcc tttctatttg tcggctagca cgtgactagc
     2161 cgattttttt atgcataaaa tgaagaagtc ttcagttgta ttgttgattc ttttaatata
     2221 gggtaataat aaaatgataa tgatatgaga agtggtggac gaaatgacag ataaaacatt
     2281 acagcaaata gataagttaa tatgttcgtg gttgaaacaa atagacaatg ttattccgca
     2341 attaattatg gaaatgacta cagaaactaa gcgtcatcga tttgatttag ttacaaatgt
     2401 agataaacag attcaacaac aattccaaca atttttagca acacattttc cggaacacca
     2461 actattagcg gaagaaaaaa gtaatgaaat gattacgaat gaaattaatc atttatggat
     2521 tatggatccc attgatggaa ctgccaattt agtaaaacag caagaagatt attgtattat
     2581 attggcgtat ttttatgaag gtaaaccaat gttatcgtac gtatatgatt atccgcataa
     2641 aaagctttat aaagcaatac gaggagaagg tgctttttgt aatggaatta agatggaaga
     2701 accaccatcg ttaaaattgg aagacgctat tatatcgttt aatgcacaag tgatgaatct
     2761 agatacggtg caagatttat ttgacgcctc atttagttat cgtttagttg gtgcttgcgg
     2821 attagattct atgcgagtgg caaaaggtca atttggggca catattaata caaatcctaa
     2881 accttgggac attgctgcac agtttttatt tgcagaacta ttgaatctaa aaatgacgac
     2941 tttagatggt aaggcaattg atcatttaaa gggtgcacct tttattataa gtaataaagc
     3001 atgtcatgaa acggtactta aaattttaaa tgcaaatggt ggttatcaaa aatacagata
     3061 gatattctct agagataaaa aatgataaaa aatagttata cgtctac
//
LOCUS       DANBWH010000245         3064 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.253, whole genome shotgun sequence.
ACCESSION   DANBWH010000245 DANBWH010000000
VERSION     DANBWH010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.253"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            40..672
                     /locus_tag="PPY92_002489"
     CDS             40..672
                     /locus_tag="PPY92_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDJ2626459.1"
                     /translation="MNHLKTYQSEDYYIHDKQFVIEGPLTYEDLKALTFDAHLTAFRD
                     AEDQYEALLEITTLPEGRIYVARQDELIVGYVTFHYPDEIERWSTGNLPYLIELGAIE
                     VSINFRQLHLAEKLIQLSLSTPEFEDYIVITTEYYWHWDLKNSKLDVFDYKKLMQRLM
                     ATGGLEIFATDDPEITSHPANCLMARIGKNITLEQQQAFDDIRYMNRFFF"
     gene            697..1866
                     /locus_tag="PPY92_002490"
     CDS             697..1866
                     /locus_tag="PPY92_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HDJ2626460.1"
                     /translation="MQQHSSKTAYVYSDKLLQYRFHDQHPFNQMRLKLTTELLLNANL
                     LSPEQIVQPRIATGDELMLIHKYDYVEAIKHASHGIISEDEAKKYGLNDEENGQFKHM
                     HRHSATIVGGALTLADLIMSGKVLNGCHLGGGLHHAQPGRASGFCIYNDIAITAQYIA
                     KEYNQRVLIIDTDAHHGDGTQWSFYADNHVTTYSIHETGKFLFPGSGHYTERGEDIGY
                     GHTVNVPLEPYTEDASFLECFKLTVEPVVKSFKPDIILSVNGVDIHYRDPLTHLNCTL
                     HSLYEIPYFVKYLADSYTNGKVIMFGGGGYNIWRVVPRAWSHVFLSLIDQPIQSGYLP
                     LEWINKWKHYSSELLPKRWEDRLNDYTYVPRTKEISEKNKKLALHIASWYESTRQ"
     gene            complement(1964..2953)
                     /gene="ccpA"
                     /locus_tag="PPY92_002491"
     CDS             complement(1964..2953)
                     /gene="ccpA"
                     /locus_tag="PPY92_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HDJ2626461.1"
                     /translation="MTVTIYDVAREARVSMATVSRVVNGNQNVKAETKNKVNEVIKRL
                     NYRPNAVARGLASKKTTTVGVIIPDISNIYYSQLARGLEDIATMYKYHSIISNSDNDP
                     EKEKEIFNNLLSKQVDGIIFLGGTITEEMKELINQSSVPVVVSGTNGKDAHIASVNID
                     FTEAAKEITGELIEKGAKSFALVGGEHSKKAQEDVLEGLTEVLNKNGLQLGDTLNCSG
                     AESYKEGVKAFAKMKGNLPDAILCISDEEAIGIMHSAMDAGIKVPEELQIISFNNTRL
                     VEMVRPQLSSVIQPLYDIGAVGMRLLTKYMNDEKIEEPNVVLPHRIEYRGTTK"
BASE COUNT          996 a          529 c          496 g         1043 t
ORIGIN      
        1 atttaataca atcacgacgt cgattgagga gttgaaaata tgaatcattt aaagacgtat
       61 caatccgaag attattacat tcatgacaag caatttgtta ttgaaggtcc tttaacatac
      121 gaagatttga aagcgcttac tttcgatgcg catttaaccg catttagaga tgctgaagat
      181 cagtatgaag ctttgttaga aattacaaca ttaccagaag gtagaattta tgttgctcgc
      241 caagatgaac tcattgtggg ttatgtcact ttccactatc ctgatgaaat tgagcgctgg
      301 tctacaggta accttccata tttaatcgaa ttgggggcaa ttgaagtcag catcaatttt
      361 aggcaattac atcttgcaga aaagctaata caacttagcc tttctacacc agaattcgag
      421 gattatatcg ttataactac tgaatattac tggcattggg atttaaaaaa ttcaaagtta
      481 gatgtatttg actataaaaa attaatgcag cggttaatgg caactggtgg acttgaaata
      541 ttcgctacag atgatccaga aataacaagt catccagcta attgtttaat ggcaagaatt
      601 ggcaaaaata ttacattaga acagcaacaa gcgtttgatg atattcgtta tatgaatcgg
      661 tttttctttt aatttttgga atatcggagg gattatatgc aacaacattc atcaaaaact
      721 gcatatgttt attcagataa gttattacaa tatcgatttc atgaccaaca tcccttcaat
      781 caaatgcgtt taaaattaac aacagagcta cttttgaatg caaatttatt gtctccagaa
      841 caaatagtac aacctagaat tgcaacaggt gacgaattaa tgttaattca taaatatgat
      901 tacgtcgaag ctattaagca tgcttcacat ggcattatca gtgaagatga ggctaagaaa
      961 tatggattaa atgatgaaga gaatggtcaa tttaagcata tgcaccgcca tagtgccaca
     1021 attgttggag gcgctttaac tttagcagat cttattatgt caggcaaagt attaaatggt
     1081 tgtcacttag gtggtggttt gcatcacgct caacctggtc gagcaagtgg tttttgtata
     1141 tacaatgata ttgcaattac cgcacaatac atagctaaag aatacaatca acgcgtttta
     1201 atcatagata ccgatgcaca tcatggagat ggtacacaat ggagtttcta tgccgataac
     1261 catgttacta cttattctat ccatgaaacc ggaaagtttc ttttcccagg ctctggtcac
     1321 tatactgaac gcggtgaaga tatcggctat ggacacactg taaatgtccc acttgaaccg
     1381 tatacagaag atgcatcatt tttggagtgt tttaaattaa cagttgagcc tgtcgtaaag
     1441 agttttaaac ctgatattat tctaagcgta aatggtgtcg atatacatta tcgtgatcca
     1501 ctaactcatc taaattgtac gttacattca ttatatgaaa ttccatattt tgtaaaatat
     1561 ttagctgatt cttatacgaa tggaaaggta attatgtttg gtggcggagg ctacaatatt
     1621 tggagagtcg taccacgtgc atggagtcat gtattcttaa gtttaattga tcaaccaatt
     1681 caaagtggtt atttaccgtt agaatggatt aataaatgga aacattattc atctgaatta
     1741 ttacctaaaa gatgggaaga tcgtttaaat gattatacct atgtcccccg cacaaaagaa
     1801 attagtgaaa aaaataaaaa attagcttta catatagcga gttggtacga atctactcgt
     1861 caataagtaa cctaatctgc aacgatttta aaagataaat aaaaggcatt cttccaacac
     1921 ccatataatt tgggtcgtta gatgaatgcc taattttgtg aatttatttt gtagttcctc
     1981 ggtattcaat tctgtgaggt aaaactacat ttggttcttc tatcttttca tcgttcatat
     2041 attttgttaa taagcgcatc cctactgcac cgatatcata taatggttga ataacactag
     2101 aaagttgtgg tctaaccatc tcaactaatc gtgtattatt gaaactaata atttgtaatt
     2161 cctctggaac tttaatacca gcatccattg cactatgcat aataccaatt gcttcttcgt
     2221 cgctgataca taaaatggca tctggcaaat tgcctttcat tttagcaaaa gcttttacgc
     2281 cttctttata actttcagca ccagaacaat tcaatgtatc acctaattga aggccatttt
     2341 tatttaacac ttcagttaaa ccttctaaaa catcttcttg agctttttta gaatgttctc
     2401 cacctactaa agcaaatgat ttagcgcctt tttcaattaa ttctcccgta atttctttcg
     2461 cagcttcagt aaaatcaata ttaactgatg ctatatgtgc atccttacca tttgttcctg
     2521 atactactac aggtacagat gattgattta tcaattcttt catttcttca gtaattgtac
     2581 caccaaggaa aataatacca tcaacctgtt tacttaataa gttattaaaa atttcttttt
     2641 ccttttcagg atcgttatct gaatttgaaa taattgagtg atatttatac attgttgcaa
     2701 tatcttcaag tccacgagca agttgtgaat aatagatatt agatatatct ggaatgatca
     2761 cacctactgt tgttgtcttt ttactagcta aacctctagc aacagcattt ggacgataat
     2821 tcaaacgctt aatgacttcg ttaactttat ttttagtttc tgctttaaca ttttggttcc
     2881 cattaacaac acgcgacact gtggccatag agacacgcgc ttctcttgct acatcatata
     2941 tagtaactgt cataatttcc tccttgtaaa cgttttattc attataataa catgttttca
     3001 tttcattttc atagtttacc atattttaat ttacagtaac atatttttgt ttggaaaaat
     3061 ttag
//
LOCUS       DANBWH010000246         3063 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.254, whole genome shotgun sequence.
ACCESSION   DANBWH010000246 DANBWH010000000
VERSION     DANBWH010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.254"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            16..1128
                     /locus_tag="PPY92_002492"
     CDS             16..1128
                     /locus_tag="PPY92_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDJ2626462.1"
                     /translation="MRRKRKWLIVICVLLVLITISQILKHFNYGTESKEAYNTYTVEK
                     ERQLDLQGKAYPREVKTYYKNNQVGTYLGVQVADGQTVKKGDMLINYKINSNKRQQLV
                     DKINDTQHIITEVYLNITKGLNNREIQKKLIEYKQTLYEAQQQLYQYDSQNRDSMYAS
                     FDGKIMIKNIDNDSDNQPILYLISKESHIKTSVTEFDLNKVKKGDKVNLSITSTGKTG
                     AGIIKQISELPISYEENLSLHENDALHLPESNNDGELANSKSISASPIFKSNNNSELS
                     KYGVIIDDLSLPIRAGYSLEIKVPLNAIKIPKSVLTKGNNVFIVNKNSIVEKRNIRIK
                     RINGDIIVEKGLKPGDKLIKQPKSTMNDGDKVEISS"
     gene            1125..1805
                     /locus_tag="PPY92_002493"
     CDS             1125..1805
                     /locus_tag="PPY92_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDJ2626463.1"
                     /translation="MIELKNVYRTFKNGDKNIRVLKDINIHINAGEFIAIMGPSGSGK
                     STLINILGFIDREYHGEYLFEGSNYQTQSDNQLANIRNKTVGFVFQNFKLIHNNTILE
                     NISMPLVYTNMNTIERKNKVMTVLSAVGLDGKGDFTPSKLSGGQQQRVAIARAIINNP
                     KFIIADEPTGALDSDTSKIIMDIFTSLNRDNHTTIILVTHDRKVAEKADRIIHILDGR
                     IQHEEVIK"
     gene            1802..2980
                     /locus_tag="PPY92_002494"
     CDS             1802..2980
                     /locus_tag="PPY92_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDJ2626464.1"
                     /translation="MRKVLNIFTIAFKSILKNKGRNIFTMIGIIIGISSVITIMSLGN
                     GFKKTAADQFSDAGAGKQEALISFTFKVDEKIKKYPFNQRDIELVNQVDGVLDAKLKE
                     NKEEGIEATITNVQKKSDIFIIKKQNLHSFKVGRGFDKEDNELRKKIVVINDQVAKTV
                     FNNNAIGKSLYIEGQGFEVIGITDKLYSDSSTVIMPENTFNYYMGHLHQGLPTLQIII
                     EDGYNKKTVVKKVESLLNKKGSGSVLGEYTYTDTEEIIKSIDKIFDSITYFVAAVAGI
                     SLFIAGIGVMNVMYISVAERTEEIAIRRAFGAKSRDIELQFLIESILICVTSGFIGLI
                     LGVVFATIIDVLTPDYIKSVVSLSSVIIAVSVSILIGLLFGWIPARAASKKELIDIIK
                     "
BASE COUNT         1193 a          368 c          573 g          929 t
ORIGIN      
        1 ataggaggac ataaattgag gcgtaaaagg aaatggctaa ttgttatttg tgtattatta
       61 gtattgatta cgatatcaca aattttgaaa cattttaact atggaactga gagtaaagaa
      121 gcatacaata catatacggt tgaaaaagaa agacaattag atttacaagg taaggcatat
      181 ccacgtgaag ttaaaacata ttataaaaat aatcaggttg gaacatattt gggtgtgcaa
      241 gtagcggacg ggcaaacagt caaaaaagga gacatgttaa ttaattataa aataaatagt
      301 aataaacgac agcaacttgt tgataagata aatgatactc aacatattat tacagaagtt
      361 tatttaaaca ttactaaagg tttaaacaat cgagagatac agaagaagtt aattgaatat
      421 aagcagacat tatatgaagc tcaacagcaa ttgtatcagt atgatagtca aaatagggat
      481 agtatgtatg catcatttga tggcaagatt atgatcaaga atatagataa tgacagtgat
      541 aatcaaccta tactgtatct catttcaaag gagtctcata ttaaaacgtc tgttacggag
      601 tttgatttaa acaaagtcaa aaaaggagac aaagttaact taagtattac gagtacgggt
      661 aaaacaggag cggggattat aaaacaaatc tctgagttgc ctattagtta tgaagagaat
      721 ttaagtcttc acgaaaatga tgccctgcat ttaccagaaa gtaacaacga tggggaatta
      781 gcaaattcaa aatcgatttc ggcatctcca atatttaaga gtaataacaa ctctgaatta
      841 tcaaagtatg gagttataat tgatgatctt agtctaccaa tacgtgcagg ttattcgttg
      901 gaaattaaag tcccattaaa tgcgattaaa atacctaaat cagtattaac aaaaggcaat
      961 aatgttttta ttgttaataa aaatagtatt gtggaaaaac ggaatattcg tataaaaaga
     1021 atcaatggtg acattattgt agaaaagggg ttaaaacctg gagacaagtt aattaaacaa
     1081 cctaaaagta caatgaatga tggtgataag gttgagattt cgtcatgata gaattaaaga
     1141 atgtttatag gacttttaaa aacggtgata aaaatattcg agttttaaaa gacattaata
     1201 tacatatcaa tgcaggtgaa ttcatagcga ttatgggacc atcaggctca gggaagagca
     1261 cactgattaa tatcttagga tttatagatc gagagtatca tggtgaatat ttgtttgaag
     1321 gaagtaatta tcagacacaa tctgataatc aattggcaaa cataagaaat aaaacagttg
     1381 gatttgtttt tcaaaatttt aaactaattc ataataatac tattttagaa aatataagta
     1441 tgccactggt atatacaaat atgaatacga tagaacgaaa gaataaagtt atgactgtgc
     1501 tcagtgctgt aggcctagat ggcaaaggtg attttactcc tagtaaacta tcaggcggtc
     1561 aacaacaacg tgttgcaatt gcgcgagcca ttataaataa tccaaagttt attattgcag
     1621 atgagccaac gggtgctttg gattcagata catctaaaat tattatggac atatttacat
     1681 cattaaaccg agataatcat actacaatta ttttagtcac acatgatcgt aaagtagcag
     1741 aaaaagctga tcgaataatt catattttgg atggtcgtat acaacatgaa gaggtgatta
     1801 aatgaggaaa gttttaaata tttttacaat agcttttaaa tcaatattga aaaataaagg
     1861 tagaaatata tttacaatga taggtataat catcggtatt tcttctgtta ttacgataat
     1921 gtctttggga aatggcttta aaaaaactgc tgctgatcaa ttttcagatg ctggcgccgg
     1981 aaaacaagaa gcgttgatta gctttacttt taaagttgat gagaaaatca aaaagtatcc
     2041 ttttaatcaa agagatatag agttggtaaa tcaagttgat ggcgtactag atgcgaagct
     2101 aaaggaaaat aaagaagaag gaattgaagc cacaataact aatgtccaga aaaaaagtga
     2161 catcttcatt ataaaaaaac aaaatttaca ctcttttaaa gtaggtagag ggtttgataa
     2221 agaggataat gaattacgta agaaaatagt ggttataaat gatcaagtag caaaaactgt
     2281 attcaataat aatgctattg gtaaatcgct atatattgag ggacagggat ttgaagtcat
     2341 aggaattacg gataaactat attctgactc atctaccgtt ataatgcctg aaaatacatt
     2401 caactactat atgggacact tacatcaagg tctgcctacc ttacagataa ttattgaaga
     2461 tggttacaat aaaaaaactg tagttaaaaa agtagaatca ctattaaata aaaaaggatc
     2521 gggctctgtt ttaggtgagt atacatatac agacactgaa gaaattatca aaagtattga
     2581 taaaattttt gatagtatta cttattttgt agcagctgtt gccggtattt cactttttat
     2641 tgctggtatt ggagttatga atgtcatgta tatatctgta gctgaaagaa cagaagaaat
     2701 tgcgatacga cgtgcatttg gtgcaaaaag tcgagatatt gaactacaat ttttaataga
     2761 gagcatttta atatgtgtga caagtggttt tattggactt attttaggtg ttgtgtttgc
     2821 aacaataatt gatgtattaa caccagatta tattaaaagt gtagtaagtt tgagttctgt
     2881 cataattgcg gttagtgtgt cgatattaat tggacttctt tttggatgga taccagctcg
     2941 cgcagcatct aagaaagagc tcatagatat tataaaatag taaatttaaa atggtaaaag
     3001 tactgaagac taattatcat attaaactga ctatcaacac caacgatgga aatgcgtatt
     3061 aga
//
LOCUS       DANBWH010000247         2951 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.255, whole genome shotgun sequence.
ACCESSION   DANBWH010000247 DANBWH010000000
VERSION     DANBWH010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.255"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            162..1397
                     /gene="arcA"
                     /locus_tag="PPY92_002495"
     CDS             162..1397
                     /gene="arcA"
                     /locus_tag="PPY92_002495"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDJ2626465.1"
                     /translation="MVQGPIQVNSEIGKLKTVLLKRPGKELENLVPDHLSGLLFDDIP
                     YLKVAQEEHDKFAQTLRDEGIEVVYLEKLAAESITEPEVRENFINDILTESKKTILGH
                     ETEIKEFFSKLSDQELVNKIMAGVRKEEIQLETTHLVEYMDDRYPFYLDPMPNLYFTR
                     DPQASIGRGMTINRMYWRARRRESIFMTYILKHHPRFKDKDVPVWLDRNSPFNIEGGD
                     ELVLSKDVLAIGISERTSAQAIEKLARNIFKDANTSFKKIVAIEIPNTRTFMHLDTVL
                     TMIDYDKFTVHAAIFKEENNMNIFTIEQNDGKDDIKITRSSKLRETLAEVLEVEKVDF
                     IPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNQLLRDKGIKVIEITGSEL
                     VRGRGGPRCMSQPLFREDI"
     gene            1483..2904
                     /gene="arcD"
                     /locus_tag="PPY92_002496"
     CDS             1483..2904
                     /gene="arcD"
                     /locus_tag="PPY92_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HDJ2626466.1"
                     /translation="MDENKLGKTSLIGLVIGSMIGGGAFNIISDMGGQAGGLAIIIGW
                     IITAIGMISLAFVFQNLTNERPDLDGGIYSYAQTGFGDFIGFSSAWGYWFAAFLGNVA
                     YATLLMSAVGNFFPIFKGGNTLPSIIIASILLWGVHFLILRGVETAAFINSIVTVAKL
                     IPIFLVIICMIVVFNFSTFKSGFYGMTSGSVGVFSWGDTMAQVKSTMLVTVWVFTGIE
                     GAVVFSGRAKSKKDVGTATVIGLISVLVIYFLMTVLAQGVIQQNQISKLANPSMAQVL
                     EHIVGHWGSVLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNKAPV
                     NALIITNILVQLFLISMLFTDSAYQFAFSLASSAILIPYTLSAFYQVKYTIQNKSKAN
                     LKQWIIGIIASIYTIWLVYAAGLDYLLLTMLLYIPGLLVYSYVQRDNNKHLTKLDYTL
                     FIFIIVLAIIGIVRLITGNISVF"
BASE COUNT         1022 a          404 c          553 g          972 t
ORIGIN      
        1 ttcactatta tatagtacaa aatacatatt catacaaaat tttgcattga ataaatctat
       61 atatgtgaaa accctcccta aacaattgtg aattaaatca cacgatgttt tttgaaagcg
      121 ttttataatg tgaatgtaat aaagcaaagg aggaatttga aatggtacaa ggacccattc
      181 aagtaaatag tgaaataggc aaattgaaaa ctgtgttgtt aaaaagacca ggaaaagaat
      241 tagaaaattt agtacctgat catttaagtg gtttattatt cgatgatatt ccctacttaa
      301 aagttgcaca agaagagcat gacaaatttg ctcaaacttt gagagatgaa ggaatcgaag
      361 tagtttattt agaaaaactt gcagcagaat ctattactga gccagaagta cgcgagaact
      421 tcataaacga tatattaaca gaatctaaaa agacaatatt aggtcatgaa actgaaatta
      481 aagaattctt ttcaaagtta tctgaccaag aacttgtaaa taaaatcatg gctggcgtac
      541 gtaaagaaga aattcaactt gaaacaactc atttagtaga atatatggat gatagatatc
      601 cattttactt agatccaatg cccaaccttt attttacaag agatccccaa gcttcaattg
      661 gtagaggaat gacaattaac agaatgtatt ggagagcacg acgtagagaa tctattttta
      721 tgacatatat actgaaacat catccaagat ttaaagataa agatgtacca gtatggttag
      781 atcgtaactc accatttaat attgaaggtg gagatgaatt agtattatcg aaagatgttt
      841 tagctattgg tatatcagaa cgtacatcag ctcaagcaat agaaaagtta gcacgtaata
      901 ttttcaaaga tgcaaacaca agttttaaaa aaatcgtagc tattgaaata cctaatacac
      961 gtacatttat gcacctagat acagtactaa ctatgattga ctacgataag tttacagtac
     1021 atgcagcaat atttaaagaa gaaaataata tgaatatatt taccatagaa caaaatgatg
     1081 gtaaggacga tataaaaatt actcgttcta gcaagttacg tgaaacactt gctgaagttt
     1141 tagaagtaga aaaagtggac tttattccaa caggtaatgg cgacgttatt gatggtgcac
     1201 gtgaacaatg gaatgatggc tcaaacacat tatgtattcg accaggggtt gtggtgacat
     1261 acgatcgcaa ctatgtatca aaccaacttt tacgcgacaa aggaattaaa gtgattgaaa
     1321 ttactggtag tgaacttgta cgtggacgcg gaggcccaag atgtatgagt cagccgttat
     1381 ttagagaaga tatttaaatt tgaaaggtgg taaaagtcac aagtaattca ttcattaaat
     1441 caaataacaa gatttttacc acctttatta ggggagatta atatggatga aaataaatta
     1501 ggtaaaactt ccttaattgg tttagtcata ggctctatga taggcggtgg tgcattcaat
     1561 atcatctcag atatgggtgg ccaagctggt ggacttgcaa taattatcgg ttggataata
     1621 actgctattg gtatgatttc tcttgctttc gtatttcaaa atttaacaaa tgagcgacca
     1681 gatcttgatg gaggaattta tagttatgct caaacagggt ttggagattt tattggtttt
     1741 tcaagtgctt ggggatattg gtttgcagca tttctaggta atgtggctta tgcaacccta
     1801 ttaatgtcag ctgtgggtaa ctttttccct atatttaaag gaggtaacac acttccaagt
     1861 attatcatag catcaatttt attatggggt gtacattttt taatacttag aggtgtagaa
     1921 actgcagcgt ttataaatag tattgttaca gtagctaaat taatacctat atttctagtt
     1981 attatatgca tgatagttgt attcaacttc agtactttta aatccggttt ttatggtatg
     2041 actagtggaa gtgttggcgt ttttagttgg ggagatacaa tggcacaagt aaaaagtact
     2101 atgttagtaa ctgtatgggt attcacaggg attgaaggag ccgttgtctt ttctggacgt
     2161 gcaaagtcta aaaaagatgt aggaactgct accgttattg gtttgatttc tgtgctagtc
     2221 atttatttct taatgactgt actagcccaa ggtgtcattc agcagaacca aatttcaaaa
     2281 cttgctaatc catcaatggc acaagtatta gaacatattg taggtcattg gggttcagtg
     2341 ttagttaata taggcttaat tatctctgtt ttaggagctt ggttaggatg gacattacta
     2401 gctggtgaat taccattcat tgtagctaaa gatggacttt tcccgaaatg gtttgctaaa
     2461 gaaaataaga ataaagctcc ggtcaacgct ttaattatta ctaatatatt agttcagtta
     2521 tttttaatta gtatgttgtt tacagatagt gcctatcagt ttgcgttttc acttgcatca
     2581 agtgcaatct taattccata tacactcagt gctttttacc aggttaaata tactattcaa
     2641 aataaatcta aagctaattt aaaacaatgg ataataggaa ttattgcatc tatttacaca
     2701 atttggttgg tttatgcagc tggattagat tatttactat taacgatgtt gttatatata
     2761 cctggattac tcgtatacag ctacgtacaa agggataata acaaacattt gacaaaattg
     2821 gattatacgt tattcatatt catcattgta cttgcaataa taggaatagt tcgtttgatt
     2881 acaggtaata tttctgtatt ttaagttatg atttaaacta agtaattttt aaggggttta
     2941 tagttatgta t
//
LOCUS       DANBWH010000248         2918 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.256, whole genome shotgun sequence.
ACCESSION   DANBWH010000248 DANBWH010000000
VERSION     DANBWH010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.256"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(156..2765)
                     /gene="adhE"
                     /locus_tag="PPY92_002497"
                     /gene_synonym="adhC"
     CDS             complement(156..2765)
                     /gene="adhE"
                     /locus_tag="PPY92_002497"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDJ2626467.1"
                     /translation="MLTIPEKENRGSKEQEVAIMIDALADKGKKALEALSKKSQEEID
                     HIVHQMSLAAVDQHMVLAKLAHEETGRGIYEDKAIKNLYASEYIWNSIKDNKTVGIIG
                     EDKEKGLTYVAEPIGVICGVTPTTNPTSTTIFKAMIAIKTGNPIIFAFHPSAQESSKR
                     AAEVVLEAAMKAGAPKDIIQWIEVPSIEATKQLMNHKGIALVLATGGSGMVKSAYSTG
                     KPALGVGPGNVPSYIEKTAHIKRAVNDIIGSKTFDNGMICASEQVVVIDKEIYKDVTN
                     EFKAHQAYFVKKDELQRLENAIMNEQKTGIKPDIVGKSAVEIAELAGIPVPENTKLII
                     AEISGVGSDYPLSREKLSPVLALVKAQSTKQAFQICEDTLHFGGLGHTAVIHTEDETL
                     QKDFGLRMKACRVLVNTPSAVGGIGDMYNELIPSLTLGCGSYGRNSISHNVSATDLLN
                     IKTIAKRRNNTQIFKVPAQIYFEENAIMSLTTMDKIEKVMIVCDPGMVEFGYTKTVEN
                     VLRQRTEQPQIKIFSEVEPNPSTNTVYKGLEMMVDFQPDTIIALGGGSAMDAAKAMWM
                     FFEHPETSFFGAKQKFLDIGKRTYKIGMPENATFICIPTTSGTGSEVTPFAVITDSET
                     NVKYPLADFALTPDVAIIDPQFVMSVPKSVTADTGMDVLTHAMESYVSVMASDYTRGL
                     SLQAIKLTFEYLKSSVEKGDKVSREKMHNASTLAGMAFANAFLGIAHSIAHKIGGEYG
                     IPHGRANAILLPHIIRYNAKDPQKHALFPKYEFFRADTDYADIAKFLGLKGNTTEALV
                     ESLAKAVYELGQSVGIEMNLKSQGVSEEELNESIDRMAELAFEDQCTTANPKEALISE
                     IKDIIQTSYDYKQ"
BASE COUNT          832 a          556 c          456 g         1074 t
ORIGIN      
        1 ttctttattt aaaaaaatga ttgtctagtt tgtatctctg aagatttggc aataaataaa
       61 agccgataac cgtaaaatga ttatcgactt aaagtttatg tggcattttt tacttttgta
      121 atttcaggtg agttatatga ttattatcag atagattatt gcttataatc atatgatgtt
      181 tgaatgatat ctttgatttc actgattagt gcttctttag gattagcagt tgtacattga
      241 tcttcaaatg cgagctctgc cattctatca attgattcat ttaattcttc ttcagacaca
      301 ccttgtgatt tcaaattcat ttcaattccg actgattgac ctaattcgta gacagcttta
      361 gctaatgatt ctacgagtgc ttctgtcgta ttccctttta atcctaagaa tttggcaata
      421 tctgcataat ctgtatctgc tctgaaaaac tcatatttag ggaataatgc atgtttttgc
      481 gggtctttgg cattataacg gataatatgc ggtagtaata tcgcattcgc tctaccatgc
      541 ggaataccat attcgccacc aattttatgt gcaattgagt gtgcaatgcc taagaatgca
      601 tttgcaaatg ccataccagc caaagttgat gcgttatgca ttttctctct tgaaacttta
      661 tcaccctttt caacagatga ttttaaatat tcgaacgtca atttaatcgc ttgtagactc
      721 aaacctcttg tatagtctga agccattaca gatacatatg attccattgc atgcgttagt
      781 acatccattc ctgtatctgc tgtaacgctt tttggcacac tcatcacaaa ttgagggtca
      841 ataattgcaa cgtcaggtgt taaagcaaaa tcagccaacg gatattttac atttgtttca
      901 ctatctgtga taactgcaaa tggtgttact tctgaacctg tacctgatgt cgtagggata
      961 caaatgaacg tcgcattttc aggcatgcct attttataag tacgtttacc gatgtctagg
     1021 aacttttgtt tagcaccgaa gaatgatgtc tcagggtgtt caaagaacat ccacattgct
     1081 tttgcagcat ccatcgctga accaccacca agtgcaatga ttgtatctgg ttggaaatca
     1141 accatcattt ccagaccttt atatactgta ttagttgatg ggttcggttc gacttcgcta
     1201 aatattttaa tttgaggctg ttccgttctt tgtcttaata cattctcaac tgtttttgta
     1261 taaccgaatt ctaccatacc agggtcacag acaatcatca ctttttcaat cttgtccatt
     1321 gttgttagac tcatgattgc attttcttca aaataaattt gagcaggcac cttgaaaatt
     1381 tgagtattat tacgtcgttt agcaatcgtt ttaatgttta ataaatctgt cgcactaaca
     1441 ttatgtgaaa ttgagtttct accgtacgaa ccacaaccta atgttaaaga cggaatcaat
     1501 tcgttataca tatcaccaat acctccaacc gctgatggtg tatttacaag tacacgacaa
     1561 gctttcattc ttagtccaaa atctttttgt aatgtttcat cttctgtatg gataacggct
     1621 gtgtgtccta atccaccaaa atgtagtgtg tcttcacaaa tttgaaatgc ttgttttgta
     1681 gattgggctt ttactaaggc taatactgga gataattttt cacgagataa cggatagtct
     1741 gaacctacac cgctaatttc ggctatgata agttttgtat tttcggggac aggtatacct
     1801 gctaattcag ctatttcaac tgcagattta ccgacaatat caggcttaat acctgttttt
     1861 tgttcattca taattgcatt ttctaagcgt tgtaattcat cttttttaac aaagtatgct
     1921 tgatgtgctt taaattcatt agtaacatct ttataaattt ctttatcaat gactacaact
     1981 tgttcagaag cacaaatcat accattatca aatgtttttg aaccaatgat atcatttact
     2041 gcacgtttaa tgtgtgctgt tttttcaatg taagacggca cgttacctgg tcccacacct
     2101 aatgccggtt tgccagttga atatgcagac ttaaccatgc ccgaaccacc tgttgctaga
     2161 actaatgcaa tacctttgtg attcattaat tgttttgttg cttcgataga aggcacttca
     2221 atccactgaa taatatcttt aggtgcacct gccttcattg ccgcttctaa tacaacttct
     2281 gctgcacgct tcgacgattc ttgtgcactt ggatggaatg caaaaatgat tggatttcct
     2341 gtcttaattg caatcatcgc tttaaaaata gttgtcgacg taggatttgt tgttggcgta
     2401 acaccacaaa taacaccaat tggttccgct acatatgtta atcctttttc tttatcttca
     2461 ccaataatcc ctactgtctt attgtctttt attgaattcc atatatattc agaagcgtat
     2521 aaatttttaa tcgctttatc ttcgtatata cctcttccag tttcttcatg tgctaatttt
     2581 gctagcacca tatgttgatc aacagctgct aagctcattt gatgaacaat atgatcaatt
     2641 tcttcttgtg actttttaga taatgcttct aatgcttttt tccctttgtc agctagagca
     2701 tcaatcataa ttgccacttc ttgttctttc gatccacgat tttctttttc aggtatagtt
     2761 aacatataca accactcctt tatactttgt gaattatttc acaaacatta tagtacatgt
     2821 ctctcaggat ataaagaaaa ttctatacaa aaaagtttaa tttcgaatat tatttgaaca
     2881 aatatcaaat tttaaaataa atgttttcat gaaatcat
//
LOCUS       DANBWH010000249         2916 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.257, whole genome shotgun sequence.
ACCESSION   DANBWH010000249 DANBWH010000000
VERSION     DANBWH010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.257"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            313..777
                     /gene="accB"
                     /locus_tag="PPY92_002498"
     CDS             313..777
                     /gene="accB"
                     /locus_tag="PPY92_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDJ2626468.1"
                     /translation="MNFKEIKELIEILDKSTLTEINIEDTKGKVTLKKEKETEIITPQ
                     ISQMPVEAAAMPMPQAQSTDSNKTEAPKPTSDNHKTINAPMVGTFYKSPSPDEEAYVQ
                     VGDTVSNETTVCILEAMKLFNEIQAEISGEIVEILVEDGQMVEYGQPLFKVK"
     gene            777..2132
                     /gene="accC"
                     /locus_tag="PPY92_002499"
     CDS             777..2132
                     /gene="accC"
                     /locus_tag="PPY92_002499"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDJ2626469.1"
                     /translation="MKKVLIANRGEIAVRIIRACRDLGIQTVAIYSEGDKDALHTQIA
                     DEAYCVGPTLSKDSYLNIPNILSIATSTGCDGVHPGYGFLAENADFAELCEACQLKFI
                     GPSYQSIQKMGIKDVAKAEMIKANVPVVPGSDGLMKDVSEAKKIAKKIGYPVIIKATA
                     GGGGKGIRVARDEKELETGFRMTEQEAQTAFGNGGLYMEKFIENFRHIEIQIVGDSYG
                     NVIHLGERDCTIQRRMQKLVEEAPSPILDDETRREMGNAAVRAAKAVNYENAGTIEFI
                     YDLNDNKFYFMEMNTRIQVEHPVTEMVTGIDLVKLQLQVAMGDVLPYKQEDIKLTGHA
                     IEFRINAENPYKNFMPSPGKIEQYLAPGGYGVRIESACYTNYTIPPYYDSMVAKLIIH
                     EPTRDEAIMAGIRALSEFVVLGIDTTIPFHIKLLNNDIFRSGKFNTNFLEQNSIMNDE
                     G"
     gene            2147..2509
                     /locus_tag="PPY92_002500"
     CDS             2147..2509
                     /locus_tag="PPY92_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDJ2626470.1"
                     /translation="MVKVTDYSNSKLGKVEIAPEVLSVIASIATSEVEGITGHFAELK
                     ETNLEKVSRKNLSRDLKIESKEDGIYIDVYCALKHGVNISKTANKIQTSIFNSISNMT
                     AIEPKQINIHITQIVIEK"
     gene            2569..>2916
                     /gene="nusB"
                     /locus_tag="PPY92_002501"
     CDS             2569..>2916
                     /gene="nusB"
                     /locus_tag="PPY92_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDJ2626471.1"
                     /translation="MSRKESRVQAFQTLFQLEMKDSDLTINEAISFIKDDNPDLDFEF
                     IHWLVSGVKDHEPVLDETISPYLKDWTIARLLKTDRIILRMATYEILHSDTPAKVVMN
                     EAVELTKQFSDDDH"
BASE COUNT         1092 a          415 c          561 g          848 t
ORIGIN      
        1 taattggtag tgaaaaaaca tagacatgag taaaggtata tatctacata tcatattgtt
       61 gaggataaaa cataatagct aataaaaata acattgatat gtttaactga atgcataaaa
      121 tttttaatca agagttaaga tgagtcaatg ttaagtaata aatacacaag tgtttttaat
      181 ttaaaatgtg gttttattat aactttggga tagacttata atgtcgccag caatatgaac
      241 atgcttgaat tgaagagttg tctcaagtaa aatagacggg tagatgaaaa caaactgaag
      301 gagtcagtaa taatgaactt taaagaaatc aaagaattaa ttgaaattct ggataaatca
      361 actttaacgg aaatcaatat tgaagatact aaaggcaaag tgacgcttaa gaaagaaaaa
      421 gaaactgaga ttatcacgcc acaaatctca caaatgccag ttgaagctgc ggcaatgcct
      481 atgcctcaag cacaatcaac tgatagcaat aaaactgaag ctccaaagcc aacttcagat
      541 aatcacaaaa caattaatgc acctatggta ggtacatttt acaaatcgcc atctccagac
      601 gaagaagcat atgtgcaagt tggggacact gtttcaaatg aaacaacagt gtgtatttta
      661 gaggcaatga aactatttaa tgaaattcaa gcagaaattt caggtgaaat tgttgaaatc
      721 ttagtagaag acggacaaat ggtagagtat ggccaaccgt tatttaaggt gaaataatga
      781 aaaaggtttt aattgcaaac cgcggtgaaa tcgcagttag gattattcgc gcttgtcgtg
      841 atttaggcat ccaaactgtt gcaatctatt ctgaagggga taaagatgcg ctacatactc
      901 aaattgctga tgaagcatat tgcgtaggtc ccactttgtc taaagattca tatttaaata
      961 ttccgaacat cttatctatt gcaacttcta caggttgtga tggcgttcat ccgggttatg
     1021 gctttttagc tgaaaatgct gactttgcag aattatgcga agcatgccaa ttgaagttca
     1081 ttggaccaag ttatcaatct atccaaaaaa tgggtatcaa agatgttgct aaggcagaaa
     1141 tgatcaaagc caatgttcca gttgttcctg gtagtgacgg tttaatgaaa gacgtctcag
     1201 aagctaagaa aatcgctaag aaaattggct atccggtcat cattaaagct actgctggtg
     1261 gtggcggaaa aggtatccgt gttgctcgtg atgaaaaaga acttgaaact ggcttccgaa
     1321 tgacagaaca agaagctcaa actgcatttg gtaatggtgg actttatatg gagaaattca
     1381 tcgaaaactt ccgccatatt gaaatccaaa ttgttgggga cagctatggt aatgtaattc
     1441 atttaggaga acgtgattgt acaattcaaa gacgtatgca gaaattagtg gaagaagcac
     1501 cttccccaat tttagatgat gaaacacgtc gtgaaatggg aaatgccgca gttcgtgcag
     1561 cgaaagctgt aaattatgaa aatgcgggaa caattgagtt tatatatgat ttaaatgata
     1621 ataaatttta ttttatggaa atgaatacac gtattcaagt agaacatcct gtaactgaaa
     1681 tggtaacagg aattgattta gttaaattac aattacaagt tgctatgggt gacgtgttac
     1741 cgtataaaca agaagatatt aaattaacag gacacgcaat tgaatttaga attaatgctg
     1801 aaaatcctta caagaacttt atgccatcac caggtaaaat tgagcaatat cttgcaccag
     1861 gtggatatgg tgttcgaata gagtcagcat gttatactaa ttatacgata ccgccatatt
     1921 atgattcgat ggtagcgaaa ttaatcatac atgaaccgac acgagatgaa gcgattatgg
     1981 ctggcattcg tgcattaagt gaatttgtgg ttcttggtat tgatacaact attccattcc
     2041 atattaaatt attgaataac gatatattta gaagcggtaa atttaataca aactttttag
     2101 agcaaaatag cattatgaat gatgaaggtt aataggaggt caatccatgg tcaaagtaac
     2161 tgattattca aattcaaaat taggtaaagt agaaatagcg ccagaagtgc tatctgttat
     2221 tgcaagtata gctacttcgg aagtcgaagg catcactggc cattttgctg aattaaaaga
     2281 aacaaattta gaaaaagtta gtcgtaaaaa tttaagccgt gatttaaaaa tcgagagtaa
     2341 agaagatggc atatatatag atgtatattg tgcattaaaa catggtgtta atatttcaaa
     2401 aactgcaaac aaaattcaaa cgtcaatttt taattcaatt tctaatatga cagcgataga
     2461 acctaagcaa attaatattc acattacaca aatcgttatt gaaaagtaat gtcataccta
     2521 attcagtaat taaataaaga aaaatacaaa cgtttgaagg agttaaaaat gagtcgtaaa
     2581 gaatcccgag tgcaagcttt tcaaacttta tttcaattag aaatgaagga cagtgattta
     2641 acgataaatg aagcgataag ttttattaaa gacgataatc cagatttaga ctttgaattt
     2701 attcattggc tagtttctgg cgttaaagat cacgaacctg tattagacga gacaattagt
     2761 ccttatttaa aagattggac tattgcacgt ttattaaaaa cggatcgtat tattttaaga
     2821 atggcaacat atgaaatatt acacagtgat acacctgcta aagtcgtaat gaatgaagca
     2881 gttgaattaa caaaacaatt cagtgatgat gatcat
//
LOCUS       DANBWH010000250         2896 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.258, whole genome shotgun sequence.
ACCESSION   DANBWH010000250 DANBWH010000000
VERSION     DANBWH010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.258"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            267..470
                     /locus_tag="PPY92_002502"
                     /pseudo
     CDS             267..470
                     /locus_tag="PPY92_002502"
                     /inference="COORDINATES: protein motif:HMM:NF023295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
     gene            440..751
                     /locus_tag="PPY92_002503"
     CDS             440..751
                     /locus_tag="PPY92_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HDJ2626472.1"
                     /translation="MCGYGRGKELGLNSDKIDFYDTLISHETEKEVIGDKNLRAIAHE
                     LTKIVKENMTVDCDKKSSSRVNKRRSVKRLLKKYGYPSDLETIAINQVVEQAEMMASN
                     Q"
     gene            complement(1022..1453)
                     /locus_tag="PPY92_002504"
     CDS             complement(1022..1453)
                     /locus_tag="PPY92_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDJ2626473.1"
                     /translation="MFKNILLPYDFENDFSAIPDYLEKVIDEDSVVVIYHVVTENDLA
                     ISVKYYNKHKEDIIREKEKKLTPFLRELEKRDIQYKIDVDFGHIKDTILEKITSGDIN
                     NGEFDLVIMSNHRVDLNIKHVLGDVTHKIAKRSSVPVLIVK"
     gene            complement(2335..2661)
                     /locus_tag="PPY92_002505"
     CDS             complement(2335..2661)
                     /locus_tag="PPY92_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDJ2626474.1"
                     /translation="MLKLYYKFNFATEPDNDYLINQKVFDAFYKALKGKQVLVYFGLF
                     KDARKKLKNGELDYLKEVDPTEYIYQIFYTWNKKEYLASEIFDLTESEEQEINHQMID
                     EIEEEK"
     gene            2677..>2896
                     /locus_tag="PPY92_002506"
     CDS             2677..>2896
                     /locus_tag="PPY92_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDJ2626475.1"
                     /translation="MNYFRYKQFNKDVITVDVSYYLRYALSYRDISEILRERGVNVHH
                     LTVYRWVQEYAPILYQIWKKKYQKAYYKW"
BASE COUNT         1061 a          386 c          403 g         1046 t
ORIGIN      
        1 tctaatacat gataaagaaa gggttaaaat aatctagttc aacttttgga ttagtggttg
       61 tattttaact tgatacagac tattctattt tttttctaaa taatgtgatt caagatatgc
      121 tatatcatct tgattaatct aaatttaatt cagagaaaaa gtctgaacgt tattataatt
      181 atacgatttc aaatacgatg gattatgtga ttagtttagg tgaagatgaa cgtcaacgtt
      241 ttattaaaac ggtcacagaa tgaggaaaag cctttgcgct ttgtgataca gaaccgaaag
      301 cgcaaaagct caatgatgaa attgtcttct ttaaagccgt taaagcagga cttgttaaat
      361 tgttacaacc atcaaaagaa ggaaaaatat ataagacact tgctaaagtt gaagcagaga
      421 ttaatcatct tgtgtcacaa tctgtggtta cggaagaggt aaagaattag ggttaaattc
      481 tgacaaaatt gatttttacg atactttaat ctcacatgaa acagaaaaag aagttatagg
      541 tgataaaaat cttagagcaa ttgcccatga attaacaaaa attgtgaaag aaaatatgac
      601 tgtagattgt gataagaaat caagtagtag agttaataag aggagatcag taaaaagatt
      661 attgaagaaa tacggatatc catctgactt agaaacaata gcaatcaatc aagtggttga
      721 acaagcagag atgatggcta gtaatcagta agaaaaacta ataaaacata ctaagattag
      781 ctatgaaaaa atctatcgtc atagatttct tcatagctaa tcttagtatg tttttagaaa
      841 agaaaattag tgaaaaaact ttacttatca tttattagtt cggtgtgctc atatcacatc
      901 ataatataag ctattatgcc gagaaaattt taataaaaaa ataaaaaaaa cctaaatact
      961 ctctcgattt gtgagaaagt atttaggctt tttgaattaa taaatttctt acttcttatg
     1021 tttatttaac aattagtact gggacagaac ttcttttagc aatcttatgt gtaacatctc
     1081 ctaaaacatg tttaatattc aaatcgactc tatgattact cataattact aaatcaaatt
     1141 caccattatt tatatctcca gaagtaattt tttctaagat tgtatcttta atatgcccaa
     1201 aatctacatc tattttatat tgaatatctc ttttttctaa ttcacgtaaa aatggagtga
     1261 gttttttttc tttttctcta ataatatctt ctttatgctt attataatat ttgacactaa
     1321 ttgcaagatc attttctgtt acaacgtgat aaattacaac aactgaatct tcatcgatga
     1381 ctttttctaa atagtcaggg atagcactaa aatcattttc gaaatcatag ggtaataata
     1441 tatttttgaa catctacacc agcctcctat tacttatata ccctgaatat atatgcttat
     1501 tctgattctg ttgcaaagta aaaaatatag ctaaccacta tttgaaattt taagatattt
     1561 ttttagagca tttagagata tttatagaat aataaacagg ttaaaaagta ataggattaa
     1621 taggatttgg ataaaaaaat caatcctatt tataggattg acgtcgacta aagcctatga
     1681 aatcaacaaa aataatagta ttcagacaat ttaaagtggt cttgtaacaa ttgtaaatag
     1741 tatgcaatat tagaggttat agtatcgatc aattcttatt taatattaac tataagtatt
     1801 gttatatcag cgtttataat aattttaaaa agagcatgtg atcaaggcga gcataagata
     1861 atccttcagc tatgctgaat accacttaag gtggtactgg atttccttat gctcatgcaa
     1921 aatcctattt tgtttgtaat atttcaaaaa gtaaaacata cattgttaaa aaataggtta
     1981 ttcttttctt cactttttac tttaatttct tatttttatt tccaaaatag tcattgttaa
     2041 taaaaattat agtaccctta tttgcaattt tgagatactt tttagagcat ttaggggtgt
     2101 tttatatatt atttatttaa tggctcttaa aagacaaata tgaactaaat ttaaaaatca
     2161 gaaagacaaa acaaaatttt ttgcttaacc agtgtcgaat tttttagaca ctgcccatct
     2221 atatgcaaat taaaaattgg cataaaatag cttaaaaatt tatcaacttt ttagttcctg
     2281 caggggcaat ttctttcaaa aaaacgctag ctttttgcta acgtttaaga aaatttattt
     2341 ttcttcttca atttcatcaa tcatttgatg attgatttct tgttcttcac tttctgttaa
     2401 atcaaaaatt tcactcgcta aatactcttt tttattccaa gtataaaaaa tttgataaat
     2461 atattcggtc ggatcaactt cttttaagta atctaattct ccatttttta atttctttct
     2521 tgcgtcttta aatagtccaa aataaacaag aacttgtttt ccttttaacg ctttataaaa
     2581 tgcatcgaac actttttgat taattaaata gtcgttatcc ggttctgttg caaagttgaa
     2641 tttatagtat aattttaaca aaaaggagtc ttctgtatga actatttcag atataaacaa
     2701 tttaacaagg atgttatcac tgtagacgtt agctactatc taagatatgc attgagttat
     2761 cgtgatatat ctgaaatatt aagggaacgt ggtgtaaacg ttcatcattt aacggtctac
     2821 cgttgggttc aagaatatgc cccaatttta tatcaaattt ggaagaaaaa gtatcaaaaa
     2881 gcttattaca aatggc
//
LOCUS       DANBWH010000251         2866 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.259, whole genome shotgun sequence.
ACCESSION   DANBWH010000251 DANBWH010000000
VERSION     DANBWH010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.259"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            133..861
                     /locus_tag="PPY92_002507"
     CDS             133..861
                     /locus_tag="PPY92_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5067 domain-containing protein"
                     /protein_id="HDJ2626476.1"
                     /translation="MKKVMGILLASTLILGACGHHQDSAKKESASHKKKENDNEELNE
                     ELKEFKSKKNMDIKIKGDTIVSDKFEAKIKEPFIINEKDEKKKYIAFKMEITAKKDDK
                     DLNPSSISHDYINITQDDKNTVNKLRDGYLLSDKKYKDWTEHNQDQIKKGKTAQAMFI
                     YELRGDGNINLNVHKYSEDKTVDSKSFKFSKLKTEDFSHRAETREEVEKKEKEFEEEY
                     KKEQEREKEKEKQKDDDHSSLDEV"
     gene            952..1199
                     /locus_tag="PPY92_002508"
                     /pseudo
     CDS             952..1199
                     /locus_tag="PPY92_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015728980.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1215..1538
                     /locus_tag="PPY92_002509"
     CDS             1215..1538
                     /locus_tag="PPY92_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626477.1"
                     /translation="MNNEELEMRLLLMKQSIEQLQEELAPNLKTRDLVLLRYMYSYKE
                     INMLDSYLFQLATNKEQITKKQFKTKLENIREVPEIPIRQVNDILEGYKNSELYVELI
                     NSILK"
BASE COUNT         1192 a          382 c          431 g          861 t
ORIGIN      
        1 gatcattatt acaagaatta tttaatatca tcttagaatc tcacaaaata tcaactttgt
       61 ttaattactc aaaatgataa gtcatttaga gtttttaaaa taaatttttg cgaaataaag
      121 gagacatgtt aaatgaaaaa agtaatgggg atattattag caagtacact tatcttaggt
      181 gcttgtggac atcatcaaga tagtgcaaaa aaagagagcg ctagtcacaa aaagaaagaa
      241 aatgacaatg aagaattaaa tgaagaactt aaagaattta aaagcaaaaa aaatatggat
      301 ataaaaatta aaggcgatac tattgttagt gacaaatttg aagctaaaat aaaagaaccg
      361 tttatcatca atgaaaaaga tgagaaaaag aaatatatcg cttttaaaat ggaaattact
      421 gctaaaaaag acgataaaga tttaaatcca tcttctattt ctcatgacta tattaatatc
      481 actcaagatg ataaaaatac agtaaataaa ttaagagatg gttatctttt aagtgataaa
      541 aaatataaag attggacaga acataaccaa gatcaaatta aaaaaggcaa aactgcacaa
      601 gccatgttta tctatgagtt aagaggtgat ggaaatatta atttaaatgt ccataaatac
      661 tcagaagata aaacagttga ttctaaatca ttcaaattta gtaaacttaa aaccgaagat
      721 ttttctcata gagcggaaac aagagaagaa gtagaaaaga aagaaaaaga atttgaagaa
      781 gagtacaaaa aagaacaaga acgagagaaa gaaaaagaaa agcaaaaaga tgacgaccac
      841 agtagtttag atgaagtata aatttggttt gttgattgtc ttacattcag ttatttaatt
      901 tttagagaaa ctgacttatt tatagcttag ccaacatgat actataaaac tatgattaaa
      961 aataaaaaat ctgtgtttaa tggtgttaaa gaaatcagtc cccgtgccgg aaaatcataa
     1021 aaaaaagatg ggataaattt gtaaatcccg cattgaataa tgcattaaaa caagaagagg
     1081 ctacatgggg aaatgtacaa ggtcaagttt cacaagcatt gatgggaact ggtattaaag
     1141 attctactgc aagaagcata gggttttggg tttcccaagt tggtcaagca ttaatataaa
     1201 aagagtgtga taaaatgaat aatgaagaat tagaaatgag attgttatta atgaaacaat
     1261 caatagaaca attacaagaa gaactagcgc ctaacttaaa aactagagat ttagtgttat
     1321 taagatatat gtattcatat aaagaaatta atatgctaga ttcttacttg ttccaattgg
     1381 ctacaaataa agagcaaata acaaaaaaac aatttaaaac aaaattggaa aacattagag
     1441 aagtaccaga aatacctatt agacaagtta atgatatatt agaaggttat aaaaatagtg
     1501 agttatatgt tgagttgata aatagcatcc taaaataaaa ataaacatac taagattagc
     1561 tatgaaggaa tctatgacga tagatttttt catagctatt ttttatagtt ataaagagga
     1621 gtagtctgtt ctgactcttg gattttaaat ctgtataata aacaagtaag ctttactctc
     1681 gccttttaaa attcgtttgt agtatgttgg gttcttaaaa tcgtgaatag gaaaatgcaa
     1741 tgagaaaggt taagtaaagt ttttaacttc tcatttattc aatggagaat tttttatcga
     1801 ttacttagac attaatatta gtaaaagaag tagtgtagtt gcaaattata aaaactaaaa
     1861 attccaaaaa gagtttatca tccaaaacaa taaaaatggc tacaatggta tgttgtgaaa
     1921 tgcatttctt aactacgact tcttttattt ccgtctcgtc aactattgct gattgtgtta
     1981 ataggcatat tgattctaat gaaaatatca aaactcggct catctcttta taactacacc
     2041 cacttagtga cgtaacaaaa atgaaaagtt ctttttcagt agccatttat ccaattaagt
     2101 gtagacgatt cacattttta tattgacata tataacatgg agaatttttt gcgtataaag
     2161 aagtttcttc atgaacaatg ttgaatgtaa aactcagaat ccaacttttt tgacacttat
     2221 ttgacacaca tcactgtccc cttatatctc aaaaactaat tcataatcac ttattactct
     2281 ttcctccatc aaataaacac ctatacaaac actgctatat caatactcac aacaaattct
     2341 gcaatgcatt atcccctgaa aaacacataa taaaaaagcc tacatccaca aaggttgtag
     2401 gctacaaata tggagacggc gggagttcat taaagcgttt atctattcat gtataactcg
     2461 ctataagcat tgtcatatca acgtttaaat tggctactct attgtgtgta aaatgttaaa
     2521 attgataaaa caatataaat ttgtgcaccc aacatcactt cataattaac gtattcaaat
     2581 acgtattcac tgcctgcaac gtgtgaggtc atatttttat atcaatgtac aaaaaactgt
     2641 ttcgccctta aaacaaccac attcctaatt aataaatagt ttagtttgat catcatataa
     2701 ttgacataaa aaaatagaca agtaccgaag tacttgccta aataacaaca agattaacat
     2761 gtgaataata atggaaataa aaagtcaacc cgaaggctaa cttacgaata gatgaaaatt
     2821 tgaacacatt gctgtgccta aaatgattat agcataaatg accaat
//
LOCUS       DANBWH010000252         2825 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.260, whole genome shotgun sequence.
ACCESSION   DANBWH010000252 DANBWH010000000
VERSION     DANBWH010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.260"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            25..2298
                     /gene="recJ"
                     /locus_tag="PPY92_002510"
     CDS             25..2298
                     /gene="recJ"
                     /locus_tag="PPY92_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDJ2626478.1"
                     /translation="MIKPKYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQ
                     AIESIISDTDVNHDALQLSDMTKAIERIKRAIANDEKILVYGDYDADGVTSTTILVTT
                     LQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLG
                     VDVIVTDHHEIGSTLPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFK
                     ALVAIGTIADLVSLTDENRSLVKQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFI
                     IGPRLNAVGRLDDASLACELLMTDDEEEAAFLAEQVEHFNRERKDIVATITEEAMAMA
                     ETKVKKGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSID
                     QVSMFEILSAHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVK
                     PVDVLLTENDITIKNIRDMNRLRPFGTDFSRPIFEMDDLSVSSVKAIGQQKNHLKLTL
                     GESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQLIIQDIAMNEQQILD
                     YRSKRKSLPFTENDENIVVLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPTSMEDL
                     SNSLQQLQFSQLYIVLQHNHSIYFDGIPNMDIFKKCYKALITKQETNIQKEGMLLCQH
                     LSVKPDTLKFMLKVFLDLKFVTQEDGLIRINQQPDKRSIDSSKVYQLRQQRMDVEKQL
                     LYQDFSEIKNWIKSQLS"
     gene            2320..>2825
                     /locus_tag="PPY92_002511"
     CDS             2320..>2825
                     /locus_tag="PPY92_002511"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009559512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDJ2626479.1"
                     /translation="MDLKQYVSEVQDWPKPGVSFKDITTIMDNGEAYGYATDKIVEYA
                     KDRDVDIVVGPEARGFIIGCPVAYSMGIGFAPVRKEGKLPREVIRYEYDLEYGTNVLT
                     MHKDAIKPGQRVLITDDLLATGGTIEAAIKLVEKLGGIVVGIAFIIELKYLNGIEKIK
                     DYDVMSLIS"
BASE COUNT         1056 a          388 c          540 g          841 t
ORIGIN      
        1 tttggaaatg aggcgtaaat atatatgatt aaaccgaagt ataagtggaa attaactaaa
       61 ccagctgaat acataagtga tgaattaaca tcaaaattaa agttaacgcc aatcgtaaaa
      121 aaaatattag aaagtaaatc gattattgat gaacaagcaa ttgaatccat catttcagac
      181 acagatgtca atcatgatgc attgcaattg agcgatatga ctaaggctat tgaaagaatt
      241 aaaagagcga tagcgaatga tgagaaaatc ctagtttatg gtgattacga tgccgacggt
      301 gttacctcaa ctacaatttt agtgacaaca ttacaacttt taggtgcaca agtaggatgg
      361 catattccta accgctttac cgaaggatat ggaccaaacg aactagcatt tagaaatgct
      421 catgatgaag gcataacatt aatcataact gttgataatg gcatacaagg tcataatgaa
      481 attaaaatgg ttcaagattt aggagttgat gtcattgtaa ctgatcatca tgaaattggt
      541 agtacattac cagaagcata tgcaatcgta catccaatgc atccatcgtt taattatccg
      601 ttccaacaat tgtgtggtgc aggtgttgca tacaaattag cacaagcatt gattgaaaat
      661 gtacctgatt attttaaagc attagttgcg ataggtacca ttgcggattt agtatcctta
      721 actgatgaaa atcgatcatt agtgaaacaa ggattaaaag tcttaaatga tcaatgtccg
      781 acctctgtta aagcattgct aaaagaagcg ggatacaatg acaatattga tgaagaaaca
      841 attggtttta ttattggacc tagacttaat gctgtaggtc gtttagatga cgcatcatta
      901 gcttgcgaat tgttgatgac tgacgacgaa gaagaagctg catttttagc agaacaagtt
      961 gaacatttta accgtgaaag aaaagatatt gtagcaacta tcacagaaga agcaatggct
     1021 atggcagaaa cgaaagttaa aaagggagat ttatttttac ttttagcaaa agaaaattgg
     1081 catgaaggtg tcttaggtat tgttgcttcg aaaattgtcg aaacatttgc tttgccaaca
     1141 ttaattttaa atattgatag ggagcaaaat catgcaaaag gttccgccag atcaattgat
     1201 caagtgtcga tgtttgaaat actatcagca catcaagagt taatcgctaa atttggcggc
     1261 catcatatgg ctgcaggtat gacgatggat attgaaaata tagaatcgtt agctgaaggt
     1321 ttaaataaat ggatgaaaga attatctaaa acaacttcgt tagatcccgt aaagccagta
     1381 gacgtattat taacagaaaa tgatataacg attaaaaata ttcgtgatat gaatcgttta
     1441 agacccttcg gaacagattt ttctagacct atttttgaaa tggatgattt atcagtttct
     1501 tcagttaagg cgatcggtca acaaaaaaat catctaaaat tgacacttgg tgaaagtaat
     1561 atcgccgcgc tattttggca aaatggacac ttagaacctg agttacaaga tgaacagcca
     1621 attaatatat taggatctgt tcaaatcaac gaatggaatg gtaatcaatc accgcaatta
     1681 attattcaag atattgcgat gaatgaacag caaatattag attatagaag taagcgaaaa
     1741 agtttacctt ttacagaaaa tgatgaaaat attgtcgtgc ttattcatcc taaaagtgat
     1801 aaagtaaatg cgaatgaata ttattatggt gaagaaatta aacaacaaac tgataaagta
     1861 gtattaagag atttaccaac gtcaatggaa gacttgtcta attccttgca acaactgcaa
     1921 ttttctcaac tttatatagt tttgcaacat aatcattcga tttacttcga tggtatacct
     1981 aatatggata tttttaaaaa gtgttataaa gcattaataa ctaaacaaga aacaaatatc
     2041 cagaaagagg gtatgttatt gtgtcaacat ttaagtgtga aaccagatac acttaaattc
     2101 atgttgaaag ttttcttaga cttaaaattt gtaacacaag aagatggttt aattcgaatc
     2161 aatcaacaac ctgataaaag atcgattgat tccagcaaag tatatcaatt aagacaacaa
     2221 cgtatggatg ttgaaaagca attattatat caagattttt cagaaataaa aaattggata
     2281 aagtcacaat tgtcgtgagc aatttaggag gaaatattaa tggatttaaa gcaatacgta
     2341 tcagaagttc aagattggcc gaaaccaggt gttagtttca aggatattac tacaattatg
     2401 gataatggtg aagcatatgg ctatgcaaca gataaaattg tagaatacgc aaaagacaga
     2461 gatgttgata tcgttgtagg acctgaagcg cgtggcttta tcattggctg tcctgtagct
     2521 tattcaatgg ggattggctt tgcacctgtt agaaaagaag ggaaattacc tcgtgaagtc
     2581 attcgttatg agtatgacct agaatatggt acaaatgttt taacaatgca caaagatgca
     2641 attaaaccag gtcaacgtgt gttaattaca gatgatttat tagctactgg tggtacgatt
     2701 gaagcagcaa taaaattagt tgaaaaatta ggcggtatcg tagtaggtat tgcatttata
     2761 attgaattga aatatttaaa tggtattgaa aaaattaaag attacgatgt tatgagttta
     2821 atctc
//
LOCUS       DANBWH010000253         2804 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.261, whole genome shotgun sequence.
ACCESSION   DANBWH010000253 DANBWH010000000
VERSION     DANBWH010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.261"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            848..1342
                     /locus_tag="PPY92_002512"
     CDS             848..1342
                     /locus_tag="PPY92_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626480.1"
                     /translation="MRRLLSLLLASTMILVACGNANNENKKKEDEKKSEVKKEATKNN
                     DKPKNEKKNQDINKNNNEQVQNVNNKQPVLNNNSNHNNQQQSNKNNYTVIENGNTVTE
                     IYNGQSHTTTNNPIREEYVEGETDPIYKREYKHFYTPEEAQRAQENSDQILREMGLEP
                     ERYE"
     gene            1420..1656
                     /locus_tag="PPY92_002513"
     CDS             1420..1656
                     /locus_tag="PPY92_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626481.1"
                     /translation="MIRFDVNGSDHANPPNNERIPTPHIHIYTEEYNNGGIAIPLKDI
                     EDLELTDEIIESLDFFMKYTNIKHDNVIKEPRLL"
     gene            1677..2453
                     /locus_tag="PPY92_002514"
     CDS             1677..2453
                     /locus_tag="PPY92_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1828 domain-containing protein"
                     /protein_id="HDJ2626482.1"
                     /translation="MKTIEERMNEYFNWLKQNYIFKELDSSTEITTPFKNHLNDFIRI
                     YADTLPNNEICLSDDGLTINELEMLGIDINTKTRTKLIQNILNQFNLKLVDKEITADV
                     KNESFAQSKHNLIQGILKIYDLTLTTKSNVTNIFYEEVFEFLYDQKIRGLAQVSVSGE
                     SGLKYSIDYIVSETKSQPEKLVNFTNNLDFNKITNEAYIYRDIKPNRPSRNKLEPIML
                     IIVNDVDHPINERAQTVAEHENLEILKWSNKSKIIETLTS"
BASE COUNT         1181 a          375 c          389 g          859 t
ORIGIN      
        1 gtttattgat ttaatattta tagacgtgat aatatcaatg tttatattaa atgaaacgaa
       61 tttgaaaatt tcgaaacagc ttaagatagc aaattgaata gtcttataga taatgcagaa
      121 tcgtctacac atagttaaac aaattatttg agaataatta ttgttaaact atgtggatag
      181 gcgattttat tttgacaaga gtcagaagaa catatattaa ggatttcaaa ttacaaatgg
      241 ctaaacttta tgaaaatggt atgactagaa tcgaaattat acgcgaatac gatttaacac
      301 tctcaatctt ctcgaactga ataaatactg ggtcattcaa tcattaagac aacttaataa
      361 gtgatgatat atgttcatac cattttgcaa atataaccag atactctcaa caaaatgtag
      421 agttcaaaat taaaaatgtc aacattatag taaactaaca tagtcccctg ttcatattct
      481 tcaatcttta aatcttatcg aacaaataaa agcagataat tttttaattt gaacgtttta
      541 ttttagaaca aaaaagagca aggttaacga gtcagaaaat ctttttcttg atacgtatat
      601 gacacttgct cttttaagaa tctgaatatt tcacgctatt tccattaaat tagcggagaa
      661 tgaatgatat ctttatacta ataaatttaa ctttttaacg tataaaatta atgcattgta
      721 ctaaagtttc aattatttta taattgtatg aatgaatcgt atacacttag gtagtcaaat
      781 ctaagaattt agatattgtt taattttaaa actatcgatt cacatataaa taaaaggaga
      841 taacagcatg aggagattac tcagtctatt actagctagt acaatgattt tagtcgcatg
      901 tgggaatgct aacaatgaaa ataagaaaaa ggaagacgaa aaaaaatcag aagtaaaaaa
      961 agaagctacg aaaaataatg ataaaccaaa gaacgaaaag aagaatcaag atataaataa
     1021 aaacaataat gagcaagttc aaaatgtcaa taataaacaa ccagtcttaa ataataatag
     1081 taatcataac aatcaacaac aaagcaataa aaataattac acagttatag aaaacgggaa
     1141 tacagttaca gaaatttata atggacaatc acacacaact acaaataacc ctattagaga
     1201 agaatatgta gagggagaaa cagacccgat atataaaaga gagtataagc atttttatac
     1261 acctgaagaa gctcaaagag cacaagaaaa tagcgaccaa attttaagag aaatgggtct
     1321 cgaaccagaa agatatgaat aaaatcaaac taaataattt cgggtagccc ccctaccctt
     1381 attatttttt tgctaagaag taataattat atttctaata tgattaggtt cgatgttaat
     1441 ggtagtgatc atgcaaatcc accaaataat gaaagaatac ctacgccaca tatacacata
     1501 tatactgaag aatataataa tggtggtatt gcgatcccat taaaagatat tgaagattta
     1561 gaattaacag atgaaattat agaatcatta gacttcttta tgaagtatac taatataaaa
     1621 cacgataatg ttataaaaga accaagatta ctgtaaaaga aatggaggga tacttagtga
     1681 agactatcga agaaagaatg aacgaatatt tcaactggtt aaagcaaaat tatatcttca
     1741 aagaattaga tagttctaca gaaattacta ctccctttaa gaaccatttg aatgatttta
     1801 ttagaatata tgctgataca ctacctaata atgaaatttg tctatcagat gacggattga
     1861 cgattaatga attagaaatg ttgggtattg atataaacac aaaaacgaga actaaactta
     1921 tacagaatat tttaaatcaa tttaatttaa aattagttga taaagaaatt acagcggatg
     1981 ttaaaaatga gagttttgct cagtctaaac ataatttaat tcaaggcata ttgaaaattt
     2041 atgaccttac tttaactact aaatctaacg ttacaaacat attctatgaa gaagtatttg
     2101 aattcttata tgaccaaaaa ataagaggtt tagcacaagt ttctgtttct ggagaatcag
     2161 gtttaaaata ttctattgat tatattgttt ctgaaacaaa atcacaacca gaaaaactag
     2221 ttaactttac aaacaactta gatttcaaca aaataactaa tgaagcttat atttaccgtg
     2281 atatcaaacc aaacagacca tccagaaaca aattagaacc tatcatgttg ataattgtaa
     2341 atgatgtaga tcatccgata aatgaaagag cacaaactgt agcagaacat gaaaaccttg
     2401 agatattaaa atggtctaat aaatcaaaaa tcattgaaac tttaacctca taaattagca
     2461 gtagtccctt atttatattc tttaatttcc aaaccatatc gtgcaaatag aaaagcagat
     2521 aactttttta tttgcacgtt ttattttaga acaagaaaga acaaggtcaa cgagtcagaa
     2581 aaactttttc ttgatacgta tttgacactt gctcttttaa gaaaactaac aattcattgt
     2641 atttccgtta aattaacgga gaattcggat tacctagata gatttacctc tagagtgtta
     2701 aaaaattgta cattcttaaa caatcataaa tgtacgttat taaattatca tttccactgg
     2761 agaatcatgt cgactaaaag attataaaag taaaaataca ttta
//
LOCUS       DANBWH010000254         2796 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.262, whole genome shotgun sequence.
ACCESSION   DANBWH010000254 DANBWH010000000
VERSION     DANBWH010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.262"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(212..610)
                     /gene="rpsI"
                     /locus_tag="PPY92_002515"
     CDS             complement(212..610)
                     /gene="rpsI"
                     /locus_tag="PPY92_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDJ2626483.1"
                     /translation="MTLAQVEYRGTGRRKNSVARVRLVPGEGNITVNNRDVREYLPFE
                     SLILDLNQPFDVTETKGNYDVLVNVHGGGFTGQAQAIRHGIARALLEADPEYRGSLKR
                     AGLLTRDPRMKERKKPGLKAARRSPQFSKR"
     gene            complement(624..1061)
                     /gene="rplM"
                     /locus_tag="PPY92_002516"
     CDS             complement(624..1061)
                     /gene="rplM"
                     /locus_tag="PPY92_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDJ2626484.1"
                     /translation="MRQTFMANESNIERKWYVIDAEGQTLGRLSSEVASILRGKNKVT
                     YTPHVDTGDYVIVINASKIEFTGNKETDKVYYRHSNHPGGIKSITAGELRRTNPERLI
                     ENSIKGMLPSTRLGEKQGKKLFVYGGAEHPHAAQQPENYELRG"
     gene            complement(1301..2104)
                     /gene="truA"
                     /locus_tag="PPY92_002517"
     CDS             complement(1301..2104)
                     /gene="truA"
                     /locus_tag="PPY92_002517"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDJ2626485.1"
                     /translation="MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRMHKRHVRI
                     HPSSRTDRGVHAIQQYFHFDTELNIPMSQWQYAMNRTLPDDIYVNNVVTVDDDFHCRY
                     DCVGKRYRYKVYQAQHRDPFQSGLKTFIPETLDLDKMNRAAQQFIGTHDFTGFCSQKT
                     EVESKVRTLYQSEIVKTDDGFDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEISDVPKL
                     LESKNRKNVPFTAPAEGLYLEKIYLDENELLKDFGNDIKIHRKKSLQND"
     gene            complement(2109..>2796)
                     /locus_tag="PPY92_002518"
     CDS             complement(2109..>2796)
                     /locus_tag="PPY92_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDJ2626486.1"
                     /translation="SPLTYLWVFALILFIMRLAKIQLWFLIKGLIPIFFFLIFTLMMH
                     IFLTKGGYVLVEWHGITIETNGILEGLYISLRLIGIVMIATIMTLSTSPIDLTDAFER
                     LLAPLKMFKLPVHQLSMIMSIALRFIPTLMDELDKIILAQKSRGSEISSGNIATRIKS
                     FIPLLVPLFISAFQRAEELAVAMEVRGYDANVKRTSYRQLKWQLRDTISLTMIIPIAI
                     ILFVLKYSGV"
BASE COUNT          855 a          516 c          456 g          969 t
ORIGIN      
        1 aaggaaaaaa attattaatt ctcttggaat ttaggttcaa aactttacgc tccaaaaaat
       61 aaaaaggaag ttaagcaatg tttagttgcc taacttccga tattgaatgc atcagtccaa
      121 tttgatatag agccaaataa ttttaaaaca cacaaacgca aaaaaacacc tcgacataat
      181 atcgaggtgt tttgtatata tcgtccgaca attaacgttt tgagaattga ggtgaacgac
      241 gagctgcttt aagacctggt tttttacgtt ctttcatacg tgggtcacga gtaagtaatc
      301 cagcgcgttt taaagaacct ctgtattcag gatctgcttc taataatgca cgagcgattc
      361 cgtgacggat agcttgagct tgtccagtga aaccaccacc atgaacgtta actaaaacat
      421 catagttacc tttagtttca gttacatcaa atggttggtt taagtctaaa attaatgatt
      481 cgaatggtaa gtattcgcgt acgtcacggt tattaactgt gatgttacct tcacctggta
      541 ctaaacgtac acgtgctact gagtttttac gacggcctgt gcctctatat tcaacttgtg
      601 ccaaagtcat ttcctccttc taattaacca cgtaattcgt agttttctgg ttgttgtgca
      661 gcgtgtggat gttcagcgcc accatataca aataattttt taccttgttt ttcgcctaaa
      721 cgagtgcttg gtaacatacc tttaattgag ttttcaatta aacgttctgg gttagttctt
      781 cttaattcac cagcagtgat tgatttgata ccacctggat gatttgagtg acggtagtaa
      841 actttgtcag tttctttgtt accagtaaat tcgatttttg atgcattaat aacgattaca
      901 taatcaccag tatcaacgtg tggtgtgtaa gttactttat ttttaccgcg taagatagat
      961 gctacttctg atgataaacg acctaatgtt tggccttcag catcgataac ataccatttg
     1021 cgctcaatgt ttgattcatt tgccataaat gtttgacgca taataattgt cctcctaaaa
     1081 aataaatgct tctattcaag ctataaacag tttcataacc taatagatga tttcatttgt
     1141 ttcattttta aaacgtttaa tttcatttat tttgttaact gttgttcgtt tctgcttata
     1201 tatcttgtaa cacaataagt ttccggggct tatcgtgggg tggatataaa acaataccgt
     1261 taataatctt ataatttcac aagctttttg tcaatggcaa tcagtcattt tgtaatgatt
     1321 ttttgcgatg tatttttata tcgttgccaa agtcttttaa taactcattt tcatctaaat
     1381 aaatcttctc taaatatagt ccttcagccg gtgcagtgaa aggaacattc ttacgattct
     1441 ttgattcaag tagcttcggc acatcagaaa tttcatgtcg cccttttcca acttctatta
     1501 aaaatgcaac taagacacgc accatgttat ataaaaatcc agatccagtg acaatataat
     1561 caaagccatc atctgtcttt acgatttcac tttggtataa tgttctaact ttgctttcta
     1621 cttcagtttt ttgcgaacaa aaccccgtaa aatcatgtgt acctatgaat tgttgcgccg
     1681 ctctgttcat tttgtctaaa tccaacgttt caggaataaa tgttttcaaa ccactttgaa
     1741 atggatcgcg gtgttgtgct tgatatactt tatatcgata gcgtttaccg acacaatcgt
     1801 aacgacaatg aaaatcgtca tcgactgtaa ctacattatt gacataaata tcatctggta
     1861 acgttcggtt cattgcatat tgccattgtg acataggtat attcagctct gtgtcgaaat
     1921 gaaagtactg ctgtatcgca tgtacacctc tatcagtcct acttgaagga tggattctca
     1981 catgtctttt atgcatgcgt tgtaatagct tttcaaattg ttgctgtacc gtacgtccat
     2041 tctgttgaat ttgaaagcct agaaaattat ttccttgata cgcaatttct actaatatac
     2101 gcatgaattt acactcctga atattttaat acgaataaaa taattgcaat tgggataatc
     2161 atggttaaag atatcgtatc tctcaattgc catttaagct gtctgtagct cgttctctta
     2221 acattggcat cataacccct aacttccatt gcgaccgcta attcttcggc gcgttggaaa
     2281 gctgagatga atagtggcac tagtaatgga ataaatgatt taatacgtgt tgcaatgttc
     2341 cctgaactta tttcagaacc acgcgacttt tgcgccaaaa taattttatc taactcatcc
     2401 attaacgtcg ggatgaatcg taacgcaatg gacattatca tacttaattg atgaactggt
     2461 aatttaaaca tctttagtgg tgcaagtaat ctttcaaacg catctgttaa atcaattgga
     2521 cttgtagata gtgtcataat tgttgcaatc atcacaatcc caattaaacg caatgatata
     2581 tatagccctt ctaaaatacc attagtttca atcgtgatgc catgccattc aactaataca
     2641 tatccacctt tagttaaaaa tatatgcatc attaatgtga agattaaaaa gaaaaatatt
     2701 ggtattaaac ctttgattag gaaccataat tgaatttttg ctaatctcat aatgaataag
     2761 ataagtgcaa acacccaaag atatgttaat ggtgaa
//
LOCUS       DANBWH010000255         2793 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.263, whole genome shotgun sequence.
ACCESSION   DANBWH010000255 DANBWH010000000
VERSION     DANBWH010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.263"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            75..869
                     /locus_tag="PPY92_002519"
     CDS             75..869
                     /locus_tag="PPY92_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDJ2626487.1"
                     /translation="MIHSKKLTLGICLVLLIILIGGCIIMTKINSRNAQIKDTFNQTL
                     NVYPTKNLDDFYDKEGFRDQEFDKRDKGTWIINSGMYIQLKGGALKSRAMVLYINRNT
                     RTAKGYFLISETTEDKKGYVHNKDKKYPVKMERNRIIPTKPITDEKLKKEIENFKFFV
                     QYGNFKDFKDYKDGDISYNPNVPSYSAKYQLNNDDYNVQQLRKRYDISTKRAPELKLR
                     GSGDLKGSSVGSKELEFNFVRNKEENVYFSDGINFKPTEEMNHEQN"
     gene            856..2181
                     /locus_tag="PPY92_002520"
     CDS             856..2181
                     /locus_tag="PPY92_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626488.1"
                     /translation="MNKTKYQINSDNIKSNSEETSAISSISYEIENANNNDLNNASIQ
                     TQIEILKSQNSFPKNLSYLKSYTDPKTGTTTSAFLNKDTGKVTLGMTGTNVHKDAILK
                     QTFGVPSYQGYIDASETLKDIGADVNIGLHSVTDKDPHYKNTQDFIKNIKKDYDIDII
                     TGHSLGGRDAMILGMSNDIKHIVVYNPAPLAIKDVSGLYADQEELKKLIEKYDGHIVR
                     FVSDEDELDAGVRNHLYETAGEKIVLKNGEGHAMSGILMSRTQAIILAELNKVKGYQD
                     ENNKALKSVRKQTRHRLHKVETLRANWIQTTGGSLSSSQQQLLEALTALTIAEGLNQL
                     VNEESQHLKKMYHAMAHKFGDNWKKAQEVGNEIGEKLTSEEVIDELRKGGAYESKLET
                     DPKRKIDDKIKKLNDVYKNCNGYIAKIKQSIEAIVSNDQMLASQIDGMM"
     gene            2181..2507
                     /locus_tag="PPY92_002521"
     CDS             2181..2507
                     /locus_tag="PPY92_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626489.1"
                     /translation="MFTTYKNINELENAYDEERKQLNDAFNQIDELRHQTRKKCEQMY
                     DHFLYFKHKMNYSEDAMIRMTRIIESFDRETNQRIRHHEMKLEDYKDELRREYLKQSD
                     RIEGDE"
     gene            2510..>2793
                     /locus_tag="PPY92_002522"
     CDS             2510..>2793
                     /locus_tag="PPY92_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626490.1"
                     /translation="MGLDFSGLPDLAVLEQMKEKEQISEVIAPEHVRMHHDHQNKLKS
                     DEKILLDQMVSHFKNFEDDFKNAAQGAWVKNATDELKDISNDLEKIQDIKV"
BASE COUNT         1153 a          373 c          516 g          751 t
ORIGIN      
        1 ttcaaaacaa tttttcgctt tagttgtaaa tattaaaaac tgagtcagaa ttataaatat
       61 aaggtggaga aatgatgatt cattcaaaaa aattaacgct cggtatatgt ttggtattac
      121 tcattatatt gattggaggt tgtatcatta tgacaaaaat aaatagtcga aatgcccaaa
      181 ttaaagacac atttaatcaa acattaaatg tgtatccaac caagaatctt gatgattttt
      241 acgataagga aggctttcga gatcaagaat ttgataaaag agataaaggg acttggatta
      301 ttaattctgg aatgtacatc caattaaaag gaggagcctt aaaatccaga gcgatggtac
      361 tttacatcaa tcgtaacact agaacagcta agggatattt tttaataagt gagacaacag
      421 aagacaaaaa aggatacgtt cataataaag ataaaaaata ccctgtcaag atggaacgta
      481 atcgaataat tccaacgaag cccataacgg acgagaagtt aaaaaaagag attgaaaact
      541 ttaagttctt tgtacaatat gggaatttta aagattttaa agattataaa gatggggata
      601 tttcatacaa tcctaacgta ccaagttatt ccgcaaagta tcaattgaat aatgatgatt
      661 ataatgtcca acaattaaga aaacgatatg atatatcaac caaaagggca cctgaattaa
      721 aattgagagg atctggcgat ttaaaaggct catccgtagg ttctaaagaa ctagaattta
      781 actttgtaag aaataaagag gaaaatgtgt atttttctga tggtattaat ttcaaaccta
      841 ctgaggaaat gaatcatgaa caaaactaaa tatcaaataa attctgacaa tataaagagt
      901 aactctgaag aaacaagcgc aatatctagt ataagttatg aaatagaaaa cgcaaataat
      961 aacgatttaa ataatgcaag tattcaaacg caaatagaaa tattaaaatc acaaaatagt
     1021 ttccctaaaa atctttcata tcttaaaagt tatacggacc ccaaaacagg tacgactaca
     1081 agcgcctttt taaataaaga cactggcaaa gttactttag gtatgacagg tactaatgta
     1141 cacaaagacg caatattaaa acaaacattt ggtgttcctt cttatcaagg atatatagat
     1201 gcgagtgaaa cgctaaagga tattggggcc gatgtcaata ttggccttca ttccgtcaca
     1261 gataaagatc cacattataa aaatacccaa gactttatca aaaatatcaa aaaagactat
     1321 gatattgata ttattaccgg acattcgctg ggcggtagag atgcgatgat tttaggtatg
     1381 agtaatgata ttaaacatat cgttgtgtat aatccagctc cattagcgat caaagatgtg
     1441 agcggccttt atgccgatca agaagaactt aaaaaattga ttgaaaagta cgatggtcac
     1501 attgtaagat ttgtgtctga tgaagacgaa ttagatgcag gtgtccgcaa tcatttatat
     1561 gaaactgctg gagaaaaaat agtacttaaa aatggagaag gccatgcaat gagtggtatt
     1621 ttaatgagca gaacacaggc tataatctta gctgaattaa acaaagttaa aggctaccaa
     1681 gacgaaaata ataaagcatt aaaatccgtt cgtaaacaaa cgaggcatag attacataaa
     1741 gtagagacgt taagagcgaa ttggattcaa acaacgggtg gatcactctc ttcctcccaa
     1801 caacaattat tagaagcttt aacagcacta accattgccg aaggcttaaa tcaattagtg
     1861 aatgaagaaa gccaacattt gaaaaaaatg tatcacgcga tggcacataa atttggagac
     1921 aactggaaaa aagcgcaaga agttggaaat gaaattggtg aaaaattaac ctctgaagag
     1981 gttatagatg aattaagaaa aggtggcgcg tatgaaagta aacttgaaac agatcccaaa
     2041 agaaaaattg atgataagat aaagaaatta aatgatgttt ataaaaattg taatggctat
     2101 atcgcaaaaa ttaaacagag tatcgaagca attgtttcta atgaccaaat gttagcgagc
     2161 cagattgatg ggatgatgta atgtttacta cgtataaaaa tattaatgaa cttgaaaatg
     2221 cctatgatga agaaagaaaa caattgaatg atgcattcaa tcaaattgat gaattaagac
     2281 atcaaacacg caagaaatgt gaacaaatgt atgatcattt cttatatttc aaacataaaa
     2341 tgaattatag tgaagacgct atgatcagga tgacacgtat tatagaatct ttcgatagag
     2401 aaacgaatca acgtatccga catcacgaaa tgaaattaga agattataaa gatgagttaa
     2461 gaagagaata tctaaaacaa tctgacagaa ttgaaggaga tgaataagcg tgggacttga
     2521 ttttagtggt ttaccagatt tagcagtatt ggaacaaatg aaggaaaaag aacagattag
     2581 tgaggttatt gcgcctgaac atgttcgtat gcatcatgat catcaaaata agctgaaaag
     2641 tgatgagaaa atattacttg accaaatggt aagtcatttc aaaaactttg aagatgattt
     2701 taaaaatgcg gcacaagggg cttgggtgaa aaatgccaca gacgaattaa aagatattag
     2761 taatgattta gaaaaaattc aagatattaa agt
//
LOCUS       DANBWH010000256         2775 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.264, whole genome shotgun sequence.
ACCESSION   DANBWH010000256 DANBWH010000000
VERSION     DANBWH010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.264"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            41..1051
                     /gene="sspA"
                     /locus_tag="PPY92_002523"
     CDS             41..1051
                     /gene="sspA"
                     /locus_tag="PPY92_002523"
                     /EC_number="3.4.21.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-specific serine endopeptidase SspA"
                     /protein_id="HDJ2626491.1"
                     /translation="MKGKFLKVSSLFVATLTTATLVSSPAANALSSKAMDNHPQQTQS
                     SKQQTPKIKKGGNLKPLEQREHANVILPNNDRHQITDTTNGHYAPVTYIQVEAPTGTF
                     IASGVVVGKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEG
                     DLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKI
                     TYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNEFNGAVFINENVRNFLKQ
                     NIEDIHFANDDQPNNPDNPDNPNNPDNPNNPDEPNNPDNPNNPDNPDNGDNNNSDNPD
                     AA"
     gene            1133..2314
                     /gene="sspB"
                     /locus_tag="PPY92_002524"
     CDS             1133..2314
                     /gene="sspB"
                     /locus_tag="PPY92_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain B"
                     /protein_id="HDJ2626492.1"
                     /translation="MNSSYKSRVFNIISIIMVSMLILSLGAFANNNKAKADSHSKQLE
                     INVKSDKVPQKVKDLAQQQFAGYAKALDKQSNAKTGKYELGEAFKIYKFNGEEDNSYY
                     YPVIKDGKIVYTLTLSPKNKDDLNKSKEDMNYSVKISNFIAKDLDQIKDKNSNITVLT
                     DEKGFYFEEDGKVRLVKATPLPGNVKEKESAKTVSAKLKQELKNTVTPTKVEENEAIQ
                     EDQVQYENTLKNFKIREQQFDNSWCAGFSMAALLNATKNTDTYNAHDIMRTLYPEVSE
                     QDLPNCSTFPNQMIEYGKSQGRDIHYQEGVPSYEQVDQLTKDNVGIMILAQSVSQNPN
                     DPHLGHALAVVGNAKINDQEKLIYWNPWDTELSIQDADSSLLHLSFNRDYNWYGSMIG
                     Y"
     gene            2352..2681
                     /gene="sspC"
                     /locus_tag="PPY92_002525"
     CDS             2352..2681
                     /gene="sspC"
                     /locus_tag="PPY92_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin B"
                     /protein_id="HDJ2626493.1"
                     /translation="MYQLQFINLVYDTTKLTHLEQTNINLFIGNWSNHQLQKSICIRH
                     GDDTSHNQYHILFIDTAHQRIKFSSIDNEEITYILDYDDTQHILMQTSSKQGIGTSRP
                     IVYERLV"
BASE COUNT         1085 a          476 c          427 g          787 t
ORIGIN      
        1 ttatatttaa cttgtaaata aattttttgg aggtttttag atgaaaggta aatttttaaa
       61 agttagttct ttattcgttg caactttgac aacagcgaca cttgtgagtt ctccagcagc
      121 aaacgcgtta tcatcaaaag ctatggacaa tcatccacaa caaacgcagt caagcaaaca
      181 gcaaacacct aagattaaaa aaggcggtaa ccttaaacca ttagaacaac gtgaacacgc
      241 aaatgttata ttaccgaata acgatcgtca ccaaatcaca gatacaacga atggtcatta
      301 tgcacccgta acttatattc aagttgaagc acctactggt acatttattg cttccggtgt
      361 agttgtaggt aaagatactc ttttaacaaa taaacacgtc gtagatgcta cgcacggtga
      421 tcctcatgct ttaaaagcat tcccttctgc aattaaccaa gacaattatc caaatggtgg
      481 tttcactgct gaacaaatca ctaaatattc aggcgaaggt gatttagcaa tagttaaatt
      541 ctcccctaat gagcaaaaca aacatattgg tgaagtagtt aaaccagcaa caatgagtaa
      601 taatgctgaa acacaagtta accaaaatat tactgtaaca ggatatcctg gtgataaacc
      661 tgtagcaaca atgtgggaaa gtaaaggaaa aatcacttac ctcaaaggcg aagctatgca
      721 atatgattta agtacaactg gtggtaactc aggttcacct gtatttaatg aaaaaaatga
      781 agtgatcgga attcattggg gcggtgtacc aaatgaattt aatggtgcgg tatttattaa
      841 tgaaaatgta cgcaacttct taaaacaaaa tattgaagat atccattttg ccaacgatga
      901 ccaacctaat aacccagata atcctgataa ccctaacaat cctgataacc ctaacaaccc
      961 agatgaacca aataaccctg acaaccctaa caaccctgat aatccagaca atggcgataa
     1021 caataattca gacaaccctg acgctgcata aacttcatct taatttagat ttagtaaata
     1081 gaagcaaagt cttagcaacg caaaatattt tgatttaaat ggagggtatt atatgaatag
     1141 ttcatataaa tctagagtat tcaatattat aagcatcata atggtttcaa tgcttatttt
     1201 atcactaggc gcatttgcta ataacaataa agccaaagcc gattcacact ctaaacagct
     1261 agaaattaat gttaagagtg acaaagtacc tcaaaaagta aaagatctag cacaacaaca
     1321 atttgctggt tatgctaaag cattagataa acaaagtaat gcaaaaactg gtaaatatga
     1381 acttggcgaa gcttttaaaa tttataaatt taatggtgaa gaagataata gttactatta
     1441 tccagttata aaagacggta aaattgttta tactttaaca cttagcccta aaaataaaga
     1501 tgatttaaac aaatcaaaag aagacatgaa ttacagtgtt aaaatttcaa acttcatcgc
     1561 taaagattta gaccaaatta aagataaaaa ttcaaatatc actgttctta ctgatgaaaa
     1621 agggttttat tttgaagaag atggcaaagt tagattagta aaagctacgc cactacctgg
     1681 taatgtaaaa gaaaaagaaa gtgctaaaac agtttcagca aaattgaaac aagagttaaa
     1741 aaatacagta acacctacta aagttgaaga aaacgaagcg atacaagaag atcaagttca
     1801 atatgaaaat acattaaaaa acttcaaaat tagagaacaa caattcgata actcatggtg
     1861 tgcaggattt agtatggcag cattattaaa tgcaactaaa aatacagaca cttataatgc
     1921 acatgatatt atgcgtacat tataccctga agtaagtgag caagaccttc ctaattgctc
     1981 aacattccct aatcaaatga ttgaatacgg taaatcacaa ggcagagata ttcattatca
     2041 agaaggcgta ccatcatatg aacaagttga tcaacttaca aaagataatg taggaattat
     2101 gattcttgca caaagtgtat ctcaaaaccc taatgatcca catttaggac atgccctagc
     2161 agttgttggt aatgctaaaa ttaatgacca agaaaaactt atttactgga atccttggga
     2221 tacagaatta tcaatccaag atgcagattc aagcctatta catttatcat tcaatcgtga
     2281 ttataactgg tatggttcaa tgataggtta ctaaaaagta atatagatat tgattaaagg
     2341 caggtaaaac tatgtatcaa ctacaattta taaatttagt ttacgacaca accaaactca
     2401 cacatctaga acaaaccaat atcaatttat tcattggtaa ttggagtaat catcaattac
     2461 aaaaatcaat ttgtatacgt catggcgatg atacaagtca caatcaatat catattcttt
     2521 ttatagatac ggcacatcaa cgcattaaat tttcatctat tgataatgaa gaaatcactt
     2581 atattcttga ttatgatgat acacagcata tcctcatgca aacgtcatcc aaacaaggta
     2641 ttggcacttc gcgcccaatc gtttatgagc gcttagtata actaatttaa atgatttcac
     2701 ttcataaagc gggttggcga gaattcaatt tctcaccagc tcgttttttc attgtaataa
     2761 taatctttaa cattt
//
LOCUS       DANBWH010000257         2765 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.265, whole genome shotgun sequence.
ACCESSION   DANBWH010000257 DANBWH010000000
VERSION     DANBWH010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.265"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            588..974
                     /locus_tag="PPY92_002526"
     CDS             588..974
                     /locus_tag="PPY92_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDJ2626494.1"
                     /translation="MAIVNKVIIVEGKSDKKRVQQVIAEPVNIICTHGTMSIDKLDDM
                     IESLYDKQVFVLADSDDEGDRIRNWFKRYLSESEHIFIDKTYCQVANCPKQYLAHVLS
                     KHGFTCKKETPLLPNINNERLVLVNE"
     gene            967..1263
                     /locus_tag="PPY92_002527"
     CDS             967..1263
                     /locus_tag="PPY92_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDJ2626495.1"
                     /translation="MNNSLDIKDVTTFYEEDKHLIFGYTPTCGTCKVSERMLDIANEI
                     LQLPLLKIDLNFYPQFCKDMQIMSTPILLLMNKDKEVKRIYAFKSVTDLLENLK"
     regulatory      1321..1424
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1513..2538
                     /locus_tag="PPY92_002528"
     CDS             1513..2538
                     /locus_tag="PPY92_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HDJ2626496.1"
                     /translation="MIELKEVVKEYRTKNKEVLAVDHVNLSIRAGSIYGVIGFSGAGK
                     STLIRMFNHLEAPTSGEVIIDGDHIGQLSKNGLRAKRQKVNMIFQHFNLLWSRTVLKN
                     IMFPLEIAGVPRRRAKQKALELVELVGLKGREKAYPSELSGGQKQRVGIARALANDPT
                     VLLCDEATSALDPQTTDEILDLLLKIREQQNLTIVLITHEMHVIRRICDEVAVMESGK
                     VIEHGPVTQVFENPQHTVTKRFVKEDLNDDFETSLTELEPLEKDAYIVRLVFAGSTTT
                     EPIVSSLSTAYDIKINILEANIKNTKNGTVGFLVLHIPYISSVDFGKFEKELIERQVK
                     MEVLRHG"
     gene            2531..>2765
                     /locus_tag="PPY92_002529"
     CDS             2531..>2765
                     /locus_tag="PPY92_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDJ2626497.1"
                     /translation="MGKSFSEIINEMITMPNIQWPEVWTAIVETLYMTVVSTIFAFIL
                     GLILGVLLFLSAKGKSIGARLFYSIVSFIVNLFR"
BASE COUNT          942 a          383 c          530 g          910 t
ORIGIN      
        1 tggggcccct gactagaatt gaaaaaagct tgttacaagc gcattttcgt tcagtcaact
       61 actgccaata taacttcgta gagcatagaa cattgattta tgtcccagcc tgttttcata
      121 agccacagct aatgtgtatt taaaaatagg aatacatgag taaaactcat gcataagaaa
      181 tactaatttc taaagaaaaa gtatttcttt atgttgggcc ccccaacttg cacattattg
      241 taagctgact ttccgccagc ttctgtgttg gggccccgcc aacttgcaca ttattgtaag
      301 ctgacttacc atcagcttct gtgttggggc cccgctataa ttgaaaagtt tgttataggt
      361 gtattttctt ttggttaact attgttaata taacattgta gattttagga tgttgatttt
      421 tgacttagcc tttgtctgct tatgttaaat gttccagcta gtcaaacatt gagtttgtaa
      481 ttattatatt aagctagttt aaattttttg tgatttatgt tttattcata ccaattttgt
      541 gaaatgatac agtgacagat gtttattgat tttaaagggt gatgatgatg gctattgtaa
      601 ataaagtgat aattgttgaa ggaaaatctg ataaaaaaag ggtgcaacag gttattgcag
      661 aaccagtcaa tattatttgt actcatggaa caatgagtat agataagctt gatgatatga
      721 tagaatcact gtatgataaa caagtttttg tattagccga ttctgatgac gaaggagatc
      781 gaattagaaa ttggtttaaa cgttatttga gtgaaagtga acatatattt attgataaaa
      841 cttactgtca agttgcgaat tgccccaaac aatatttggc gcatgtactt tcaaaacatg
      901 gctttacttg taagaaagaa acacctcttt taccgaatat aaataatgaa aggttagttt
      961 tagtaaatga ataattcatt agacatcaaa gatgtaacta cattttatga ggaagacaaa
     1021 catttaatct ttggttatac accaacgtgt ggtacttgta aggtttcaga aagaatgtta
     1081 gacattgcta atgaaatatt gcagttacca ttattgaaaa tagatttaaa cttttatcct
     1141 cagttttgta aagatatgca aatcatgtct acgccgattt tattgttgat gaataaagat
     1201 aaagaagtaa aacgaattta tgcatttaaa tcggtgactg atttgttaga aaatttaaaa
     1261 tagttattga cgaaatttta ctaaggtgtt aggattaata caaattaaat gatgcgtaaa
     1321 ctcttatcga gagtggtgga gggatgtgcc ctacgaagcc cggcaaccgt cttatataga
     1381 aatggtgcca attcacataa agttttaact tttgaagatg agagaaacaa tactactatt
     1441 gctttctcaa tttttctatc gatattgaga aagcattttt tattttatta agcaacacag
     1501 ggaggaatca acgtgattga attaaaagaa gttgttaaag aatatcggac taaaaataaa
     1561 gaagtccttg ctgtagatca cgttaattta tcgattcgag caggatcgat ttatggcgtc
     1621 attggttttt ctggagcagg aaaaagtact ttgattcgaa tgtttaatca tttagaagcg
     1681 cctacatcag gtgaagttat tatagatgga gaccatatag gtcaattgtc caaaaatgga
     1741 ttaagagcaa aaagacaaaa agtaaatatg atcttccaac attttaattt gttatggtca
     1801 aggactgtgt taaaaaatat tatgtttccg cttgaaattg caggtgtccc tagaaggaga
     1861 gctaagcaaa aagcattaga acttgtcgaa ctcgtcggtt taaaaggtag agaaaaggct
     1921 tatccatcag agttatcagg tggacaaaag caacgtgttg ggattgcacg agcgttagct
     1981 aatgatccaa cggtcttgct ttgtgatgag gcaacaagtg cacttgatcc gcaaacaaca
     2041 gatgaaattt tagatctact actaaaaatt agagaacaac aaaatttaac aattgtacta
     2101 attacgcatg aaatgcatgt cattcgtcgt atttgtgatg aagttgcagt tatggaaagt
     2161 ggtaaagtga tagaacatgg accggtgaca caggtttttg aaaatccgca acacactgtg
     2221 acaaaacgat ttgtgaaaga agatttaaat gatgatttcg aaacatcttt aacagaatta
     2281 gagccattag aaaaagatgc ttatatcgtt agattagttt tcgctggttc aacaacaacc
     2341 gagcctattg tatcgagtct atcaactgcc tatgatatta aaattaatat tttagaagca
     2401 aatattaaaa atacaaaaaa tggaacagtc ggctttttag ttctgcatat tccatatatt
     2461 tcaagtgtag atttcggaaa attcgaaaaa gagttaattg agcgacaagt taaaatggag
     2521 gtgttaagac atgggtaaat catttagtga aattataaat gaaatgatta caatgcctaa
     2581 tattcagtgg ccagaagttt ggactgcaat agtcgaaaca ctatacatga cagtcgtctc
     2641 aactatattt gcatttatac tcggtcttat tttaggtgtg ttattattct tgtctgctaa
     2701 aggtaagtct atcggtgcaa ggttatttta ttctatcgtt tctttcattg ttaacttatt
     2761 tagag
//
LOCUS       DANBWH010000258         2763 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.266, whole genome shotgun sequence.
ACCESSION   DANBWH010000258 DANBWH010000000
VERSION     DANBWH010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.266"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            159..362
                     /locus_tag="PPY92_002530"
     CDS             159..362
                     /locus_tag="PPY92_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HDJ2626498.1"
                     /translation="MTFYNFIMGFQNDNTPFGILAEHVSEDKAFPRLEERHQVIRAYV
                     MSNYTDHQLIETTNRAISLYMAN"
     gene            complement(359..595)
                     /locus_tag="PPY92_002531"
     CDS             complement(359..595)
                     /locus_tag="PPY92_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626499.1"
                     /translation="MDHHNQCNYVNPQNVSLDWECFIISKSEMLLDGVPNELINTWLD
                     KDIITPFSIRNDEINFKTKDIWDALIHHNWYYSN"
     gene            822..1403
                     /locus_tag="PPY92_002532"
     CDS             822..1403
                     /locus_tag="PPY92_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDJ2626500.1"
                     /translation="MAKTLYLMRHGQTLFNFKGLIQGFGDSPLTELGIAQAQKARSYY
                     ETKGINFDLYASSTQERASDTLENVAPNQSYQRFKGLKEWHFGIFEGESVYLFDNLYK
                     PEDLFGDRIVPFKGEARQQVEERIVKTLHDIMSQTKNNALVVSHGTIMGVFLRYCLKL
                     DEALKHNIGNCNILKFEYDNGTFKFVELIDPNL"
     gene            complement(1476..2092)
                     /locus_tag="PPY92_002533"
                     /pseudo
     CDS             complement(1476..2092)
                     /locus_tag="PPY92_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
     gene            complement(2247..2741)
                     /locus_tag="PPY92_002534"
     CDS             complement(2247..2741)
                     /locus_tag="PPY92_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDJ2626501.1"
                     /translation="MIEIKTLTNNDFNEYKRLVSTVNEEFTQDSHYSQTMTDTLIHDI
                     LNQGSPKCIVFGCYENETLIATAALEQIRYVGKEHKSLIKYNFVTNNDKSINSELINF
                     IINYARQNNYESLLTSIVSNNIGAKVFYSALGFDILGFEKNAIKIGNTYFDEHWLFYD
                     LINK"
BASE COUNT         1000 a          415 c          413 g          935 t
ORIGIN      
        1 ctaaaaactc ttaaagttgt ataattcgtt aatattttta gaatcagata tcaaaaaaat
       61 gtgcaaaatc acttttgcac atttacaaat actagttttc tgataagatt aataataaca
      121 ctttaaatag cgcttaataa atgaaggggg caagtcttat gacgttttac aatttcatca
      181 tgggttttca aaatgataac acaccatttg gtatattggc cgaacacgtt agtgaagata
      241 aagcattccc tcgattagaa gaaagacacc aagtaattag agcatatgtg atgtctaatt
      301 acacagatca tcaattaatt gaaactacaa atagagctat tagcttatat atggcaaatt
      361 aatttgagta gtaccaatta tgatgtatta gtgcatccca aatatctttt gttttaaagt
      421 ttatttcatc atttcttatc gaaaatggtg taataatgtc tttatctaac caagtgttga
      481 taagttcatt tggtacacca tctaacaaca tttcactttt actaattata aaacattccc
      541 agtcaagtga aacattttgt ggattcacat aattacattg attatgatga tccataaaca
      601 ctcactcctt taaaattctg tactcttcat tgcgttttac cccgtcacat tatcttttaa
      661 actaaaatca tcattactta tgaaaaaaat gtacatcaaa agcaaaggtt ttcgctaccg
      721 aaaaagttta aataatggtt taatatattt ggtactcatt ttaataaaaa gagaatacat
      781 tttgagctat caatacattt tttattgaag aggtgttatt gttggctaaa acgttatatt
      841 taatgcgcca cggacaaact ttgtttaatt ttaagggact aattcaggga tttggagatt
      901 cgccgctaac agaacttgga attgctcaag ctcaaaaggc acgtagttat tatgaaacta
      961 aggggataaa cttcgattta tatgcatcat caacgcaaga acgcgcaagt gacacacttg
     1021 aaaatgttgc acctaaccaa tcgtatcaac gttttaaggg actgaaggaa tggcattttg
     1081 gaatatttga aggtgagtca gtctatctat ttgataatct atacaagcct gaggacttat
     1141 tcggagatcg aattgttcct ttcaaaggag aggcaaggca acaagttgaa gaacgcattg
     1201 tgaaaacttt acatgacatt atgtctcaaa caaagaataa tgcattagtc gtgagtcatg
     1261 gaacaataat gggagtattt ttaagatatt gccttaaact agatgaagca ttaaagcata
     1321 atatcggtaa ttgtaatatc ctgaaatttg aatatgacaa tggaacattt aaatttgttg
     1381 agttaattga tccaaattta taataaataa agcgttatac atagacacaa gtatgtcttt
     1441 tgtataacgc ttttaaaata caactacata acgctttaga ataataattg aataagtttt
     1501 tgtaatatca ttagagcaac aataataatt attatgcttg atattttatt gattattgtt
     1561 aataattttc cagttttatc aattgatcca accatttttc ctaaaattgc gagtaaaaag
     1621 aaccataacc atgaaacgct aatacaagcg attgtaaacg caattttatt gctgccacta
     1681 tataatgcag cactactacc aattacacca attgtatcta aaatagcatg tggattgagt
     1741 aatgaaactg ataaagcaaa acttacttgt ttcattggag atataatttg agcttctcca
     1801 tctgttgagg tttatcatgc caaatggtcc aagccatata catcaagaaa attaaaccaa
     1861 ctatataaat aattgcttga agtacaggta aagacataat aatgatagat actcctacca
     1921 ctgcaataat aataagtaag ctgtctgaca acccggctgt aattattgca ggcaatacat
     1981 atctatattt tggttgatta gctccttggt taaaaataaa tacattttgt gcacctaaag
     2041 gtaaaattag accaatcgct aagataaatc catgaataat tgcggttacc attaagtttt
     2101 gacaccccta aaacatagaa tattctgaaa aaacttattg ttatcatatc aaataaaaaa
     2161 caactactca atagtagagt agttgtatga aaaatagaac ctatatgatt tgaatttatg
     2221 agaaatgtag aatatataaa actgtattac ttattaatca aatcataaaa aagccaatgt
     2281 tcatcgaaat aggtatttcc gattttaatt gcatttttct caaaaccaag aatgtcgaat
     2341 cctagtgcac tatagaaaac tttagcacct atgttgtttg acacaattga tgtaagtaaa
     2401 gattcgtaat tgttctgccg tgcataatta ataatgaaat taatgagctc gctattaatc
     2461 gatttatcgt tattagtaac aaagttgtat ttaattaatg atttatgttc ttttccaacg
     2521 tatcgaattt gttctaaggc agctgttgcg ataagtgttt cgttttcata acagccaaat
     2581 acaatacatt tcggtgaacc ttgatttaaa atgtcatgta ttaaggtgtc agtcattgtt
     2641 tgactataat gtgaatcttg agtgaattct tcattgactg tcgaaacaag tctcttatac
     2701 tcattaaaat cattattcgt taatgtttta atttcaatca ttactgacct ccttaatcat
     2761 tat
//
LOCUS       DANBWH010000259         2679 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.267, whole genome shotgun sequence.
ACCESSION   DANBWH010000259 DANBWH010000000
VERSION     DANBWH010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.267"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            29..2572
                     /locus_tag="PPY92_002535"
     CDS             29..2572
                     /locus_tag="PPY92_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding adhesin SdrG C-terminal
                     domain-containing protein"
                     /protein_id="HDJ2626502.1"
                     /translation="MIPNRLNKFSIRKYSVGTASILVGTTLIFGLSGHEAKAAEHTNG
                     ELNQSKNETTAPSENKTTEKVDSRQLKDNTQTATADQPKVTMSDSATVKETSSNMQSP
                     QNATASQSTTQTSNVTTNDKSSTTYSNETDKSNLTQAKNVSTTPKTTTIKQRALNRMA
                     VNTVAAPQQGTNVNDKVHFTNIDIAIDKGHVNKTTGNTEFWATSSDVLKLKANYTIDD
                     SVKEGDTFTFKYGQYFRPGSVRLPSQTQNLYNAQGNIIAKGIYDSKTNTTTYTFTNYV
                     DQYTNVSGSFEQVAFAKRENATTDKTAYKMEVTLGNDTYSKDVIVDYGNQKGQQLISS
                     TNYINNEDLSRNMTVYVNQPKKTYTKETFVTNLTGYKFNPDAKNFKIYEVTDQNQFVD
                     SFTPDTSKLKDVTGQFDVIYSNDNKTATVDLLNGQSSSDKQYIIQQVAYPDNSSTDNG
                     KIDYTLETQNGKSSWSNSYSNVNGSSTANGDQKKYNLGDYVWEDTNKDGKQDANEKGI
                     KGVYVILKDSNGKELDRTTTDENGKYQFTGLSNGTYSVEFSTPAGYTPTTANAGTDDA
                     VDSDGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKGVKV
                     TLQNEKGEVIGTTETDENGKYRFDNLDSGKYKVIFEKPAGLTQTGTNTTEDDKDADGG
                     EVDVTITDHDDFTLDNGYYEEETSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSDSDAGKHTPTKPMS
                     TVKDQHKTAKALPETGSENNNSNNGTLFGGLFAALGSLLLFGRRKKQNK"
BASE COUNT         1070 a          413 c          491 g          705 t
ORIGIN      
        1 taaaaagaca gcaacaaata gaaaaggcat gataccaaat cgattaaaca aattttcgat
       61 aagaaagtat tctgtaggta ctgcttcaat tttagtaggg acaacattga tttttgggtt
      121 aagtggtcat gaagctaaag cggcagaaca tacgaatgga gaattaaatc aatcaaaaaa
      181 tgaaacgaca gccccaagtg agaataaaac aactgaaaaa gttgatagtc gtcaactaaa
      241 agacaatacg caaactgcaa ctgcagatca gcctaaagtg acaatgagtg atagtgcaac
      301 agttaaagaa actagtagta acatgcaatc accacaaaac gctacagcta gtcaatctac
      361 tacacaaact agcaatgtaa caacaaatga taaatcatca actacatata gtaatgaaac
      421 tgataaaagt aatttaacac aagcaaaaaa cgtttcaact acacctaaaa caacgactat
      481 taaacaaaga gctttaaatc gcatggcagt gaatactgtt gcagctccac aacaaggaac
      541 aaatgttaat gataaagtac attttacgaa cattgatatt gcgattgata aaggacatgt
      601 taataaaaca acaggaaata ctgaattttg ggcaacttca agtgatgttt taaaattaaa
      661 agcgaattac acaatcgatg attctgttaa agagggcgat acatttactt ttaaatatgg
      721 tcaatatttc cgtccaggtt ctgtaagatt accttcacaa actcaaaatt tatataatgc
      781 ccaaggtaat attattgcaa aaggtattta cgatagtaaa acaaatacaa caacgtatac
      841 ttttacgaat tatgtagatc aatacacaaa tgttagcggt agctttgaac aagtcgcatt
      901 tgcgaaacgt gaaaatgcaa caactgataa aactgcttat aaaatggaag taactttagg
      961 taatgataca tatagtaaag atgtcattgt cgattatggt aatcaaaaag gtcaacaact
     1021 tatttcgagt acaaattata ttaataatga agatttgtca cgtaatatga ctgtttatgt
     1081 aaatcaacct aaaaagacct atacaaaaga aacatttgta acaaatttaa ctggttataa
     1141 atttaatcca gatgctaaaa acttcaaaat ttacgaagtg acagatcaaa atcaatttgt
     1201 ggatagtttc accccagata cttcaaaact taaagatgtt actggtcaat tcgatgttat
     1261 ttatagtaat gataataaga cggcgacagt agatttattg aatggtcaat ctagtagtga
     1321 taaacagtac atcattcaac aagttgctta tccagataat agttcaacag ataatgggaa
     1381 aattgattat actttagaaa cacaaaatgg aaaaagtagt tggtcaaaca gttattcaaa
     1441 tgtgaatggc tcatcaactg caaatggcga ccaaaagaaa tataatctag gtgactatgt
     1501 atgggaagat acaaataaag atggtaaaca agatgccaat gaaaaaggga ttaaaggtgt
     1561 ttatgtcatt cttaaagata gtaacggtaa agaattagat cgtacgacaa cagatgaaaa
     1621 tggtaaatat cagttcactg gtttaagcaa tggaacttat agtgtagagt tttcaacacc
     1681 agccggttat acaccgacaa ctgcaaatgc aggtacagat gatgctgtag attctgatgg
     1741 actaactaca acaggtgtca ttaaagacgc tgacaacatg acattagata gtggattcta
     1801 caaaacacca aaatatagtt taggtgatta tgtttggtac gacagtaata aagatggtaa
     1861 acaagattcg actgaaaaag gaattaaagg tgttaaagtt actttgcaaa acgaaaaagg
     1921 cgaagtaatt ggtacaactg aaacagatga aaatggtaaa taccgctttg ataatttaga
     1981 tagtggtaaa tacaaagtta tctttgaaaa gcctgctggt ttaactcaaa caggtacaaa
     2041 tacaactgaa gatgataaag atgccgatgg tggcgaagtt gatgtaacaa ttacggatca
     2101 tgatgatttc acacttgata atggctacta cgaagaagaa acatcagata gtgactcaga
     2161 ttcggacagc gattcagact cagatagcga ctcagattca gatagtgact cagactcaga
     2221 tagcgactca gactcagata gcgactcaga cagcgactca gactcagata gtgattcaga
     2281 ttcggacagc gactcagatt cagacagcga atcagattcg gatagcgatt cagactcaga
     2341 tagcgactca gacagcgatt cagattcgga tagcgattca gattcagatg caggtaaaca
     2401 tactccgact aaaccaatga gtacggttaa agatcagcat aaaacagcta aagcattacc
     2461 agaaacaggt agtgaaaata ataattcaaa taatggcaca ttattcggtg gattattcgc
     2521 ggcattagga tcattattgt tattcggtcg tcgtaaaaaa caaaataaat aatatgatta
     2581 acttaaccag gtccatgtgg cctggttttt tctgtttaga aattcccaga tataaaacaa
     2641 aaaatcttta aggaatagat gcaatttaat ttgttacag
//
LOCUS       DANBWH010000260         2654 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.268, whole genome shotgun sequence.
ACCESSION   DANBWH010000260 DANBWH010000000
VERSION     DANBWH010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.268"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            56..208
                     /locus_tag="PPY92_002536"
     CDS             56..208
                     /locus_tag="PPY92_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626503.1"
                     /translation="MVPEEKGSITLSKEAAIIFATAKFKPFKNRIKNNPLKTNPFLKL
                     HENKKS"
     gene            469..741
                     /locus_tag="PPY92_002537"
     CDS             469..741
                     /locus_tag="PPY92_002537"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDJ2626504.1"
                     /translation="MTKERRTFSSEFKLQMVRLYKNGKLKNEIIRKYDLKPSIISNSI
                     KQHQNTGPFNHQDNLKSDEKELIKLRKEVQHLKMEHDVLKQILKLI"
     gene            1140..1844
                     /locus_tag="PPY92_002538"
     CDS             1140..1844
                     /locus_tag="PPY92_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDJ2626505.1"
                     /translation="MSKNINQANSKLNTSNKKLKQAYSKSDSKNMKVIYMPHWLEFYS
                     IAIHSDVTSNKKRYYKSFSRGTVVYVKLGSNIGSEFSGNHFCVILDNKDNKGKETVTI
                     VPLSSKGNKNYLKLNESVLNLTATDLKKQIIDISSKVQALTQKYRDIDIKLSTEDKKL
                     LSNLNQKANELYRVIGVYSKHKGKDTYVNISAITTISKRRISKINDSDPTGTIIISED
                     DMNEIEKQLKIKFFSN"
     gene            2236..>2654
                     /locus_tag="PPY92_002539"
     CDS             2236..>2654
                     /locus_tag="PPY92_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626506.1"
                     /translation="MHNKNEFNKKLKDFIGSDKRMALVKGYINEYKLTTVLRALNNSD
                     YTKGTIHTRSIGNLKQIVDNRIIPKNITQNKIFKLSNLTLEVNLYERNTIYHGDFAIY
                     YPVESALIIETDTPRLINHINNNPVQKIFIITTNDWS"
BASE COUNT         1125 a          386 c          360 g          783 t
ORIGIN      
        1 gtatagtaaa attattttat aagatttaaa tacaattgta caaggagtga atatcatggt
       61 accagaagaa aaaggttcta ttactttgtc aaaagaagct gctatcatat ttgcaaccgc
      121 aaaattcaaa ccatttaaga acagaattaa aaataaccca ctaaaaacaa atccatttct
      181 taaattacat gaaaacaaaa aatcttaata actttattta tagcatttct aatctcagaa
      241 atgctataaa ggacaacgcc ctctttcata actatataag tcaagaaacc aagacatttc
      301 aaacatcttt aaacccttca atagcacatt ccctaaaaca taaagcaata acaatgaacg
      361 atcaaaattc cttacgtact tatgatgtgt cagtgaatcg tctacactta attgaacaaa
      421 ttctatgaga atagatattg ttaaattaag aaagtagtca atcttattat gacaaaagaa
      481 agaagaacat ttagttcaga gtttaagtta caaatggtta gattatataa aaatggtaag
      541 cttaagaatg aaattatacg aaagtatgat ttaaaaccct caattatctc aaattcgata
      601 aaacaacacc aaaatactgg acccttcaat catcaagata atttaaaaag tgatgaaaaa
      661 gagttaataa aattacgcaa agaagttcaa catttaaaaa tggaacatga tgttttaaag
      721 caaattttaa agttgattta gaaactgaac tttttaaaga tacattactt gaagattcta
      781 atttatctga ttatataaag aaccaacttc caacgatatg gagagactaa gaaatagttt
      841 aaatttattt aatttcaata aaaactcata ataatattta agtaagttag attgctttta
      901 gcattaggaa ttcgcttata attagatgtt caatatagac ttttttacac atacatgaac
      961 tataaaaagc gaataaatag aaggtcattg cgaaatttgc taaatacaaa attgatgagt
     1021 tccacctaat aaaaattgtt ggttttatag taacatctat tttcgtacca ttcatgttaa
     1081 tttcagtgct aataatgctg atataaagaa gaataaattg attaggagaa cactatttaa
     1141 tgtctaaaaa tatcaatcaa gcaaatagta aattaaatac gtcaaacaaa aaactaaaac
     1201 aagcttattc aaaatccgat agtaaaaaca tgaaagttat atacatgcct cattggctag
     1261 agttttattc aattgctata catagcgatg ttacaagcaa taaaaaaaga tattataaat
     1321 catttagtag aggaacagtg gtatatgtaa aactagggag taatattgga agtgagtttt
     1381 ctggtaatca tttttgtgta atattagata acaaagacaa taaaggaaaa gaaacagtta
     1441 caatagttcc actttcttcc aaaggtaata aaaattattt gaagttaaac gaatcggtgc
     1501 taaatctaac agcaacagat ttaaaaaaac agattatcga tatcagtagt aaagtacaag
     1561 cattaaccca aaaatataga gatattgaca ttaaacttag cacagaagat aaaaagcttt
     1621 tgtctaactt aaatcaaaaa gctaatgaac tgtatagagt gataggtgtt tattccaagc
     1681 acaaagggaa agatacatac gttaatatat cagcaattac aacaataagc aaaagacgta
     1741 taagcaaaat aaatgattct gatcctactg gaacgataat aatttcagaa gatgacatga
     1801 atgaaatcga aaagcaatta aagataaagt tcttttctaa ttgaattgta atattacatc
     1861 tgatataatt gatttacaaa ttcccggtaa ccattccggc ttcattctct tgaagaatga
     1921 catattctca gcgttttagc tgaaggtcag atgatacgtc atctggcctc tttttttata
     1981 taaatgccct ttaagtgaaa tatacaacaa tctgcaacca atatagacat cacaaaacac
     2041 acaataaatt acacaatgtg ttataagcca catgtcatta ttatcgtagc tagctgcgtc
     2101 actcaaaacg aatacggaac gcgttggtta ttccaatcat aatattactc tgcaaataca
     2161 cagccctgta tccgagtaaa tgaatagaga tttgaacaaa tgaaaacaat caactaatgg
     2221 aaaggataaa atattatgca caacaaaaat gaattcaaca aaaaactgaa agattttata
     2281 ggtagcgata aacggatggc tttagtaaag ggttatatta acgagtataa attaacaact
     2341 gttttaagag ctttaaataa ttccgattac acaaaaggga cgatacacac tcgttcaata
     2401 ggcaacctaa aacaaatagt agataatcga attattccta aaaatataac tcaaaacaaa
     2461 atcttcaaat tgagtaattt aaccttagag gttaacctct atgaaagaaa cacaatttat
     2521 cacggagact ttgctattta ctatcctgta gaatctgctc taataataga aacagatact
     2581 cctaggctaa ttaatcatat aaataacaac ccagtccaaa aaatatttat aataacaaca
     2641 aatgactgga gctt
//
LOCUS       DANBWH010000261         2634 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.269, whole genome shotgun sequence.
ACCESSION   DANBWH010000261 DANBWH010000000
VERSION     DANBWH010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.269"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            78..473
                     /gene="spxA"
                     /locus_tag="PPY92_002540"
     CDS             78..473
                     /gene="spxA"
                     /locus_tag="PPY92_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDJ2626507.1"
                     /translation="MVTLFTSPSCTSCRKAKAWLQEHDIPYTERNIFSEHLTIDEIKQ
                     ILKMTEDGTDEIISTRSKTYQKLNVDIDSLPLQDLYSIIQDNPGLLRRPIILDNKRLQ
                     VGYNEDEIRRFLPRKVRTFQLQEAQRMVD"
     gene            844..1563
                     /gene="mecA"
                     /locus_tag="PPY92_002541"
     CDS             844..1563
                     /gene="mecA"
                     /locus_tag="PPY92_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HDJ2626508.1"
                     /translation="MRIERVDDTTVKLFITYSDIEARGFSREDLWTNRKRGEEFFWSM
                     MDEINEEEDFVVEGPLWIQVHAFEKGVEVTISKSKNEDMMNMSDDDATDQFDEQVQEL
                     LAQTLEGEDQLEELFEQRTKEKEAQGSKRQKSSARKNTRTIIVKFNDLEDVINYAYHS
                     NPITTEFEDLLYMVDGTYYYAVHFDSHVDQEVINDSYSQLLEFAYPTDRTEVYLNDYA
                     KIIMSHNVTAQVRRYFPETTE"
     gene            1684..>2634
                     /locus_tag="PPY92_002542"
     CDS             1684..>2634
                     /locus_tag="PPY92_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HDJ2626509.1"
                     /translation="MLVALNEEKERVLATTALRKTQYFCPVCGKQVILKRGLKVISHF
                     AHKHLAEQKCFNNETIKHYKSKLILAQMIQQQGCKVEIEPFLKEIKQIPDILINNKYV
                     IELQYSPIPYKQILQRTEGLKKMGYKVSWLLNDVDYCHNKVKFNHFHSLFINPITRKL
                     HTFNLEKKQIMMFQQIQYLGGHKYVAEKRNAKIIELFNEAPCDYHAVYKLSKFAINQY
                     IKYCRWQNSVLEPTLSAMYQLQLTDQEVVYNYGYIFPEQIYIENHPIEWQLQVDLWLK
                     NGKSKLVNDNLNYFKLKKFIVALESKTAIIEKLINNYLNIC"
BASE COUNT         1000 a          327 c          442 g          865 t
ORIGIN      
        1 aaaaaaatgt tttttcttga tctataacta aattttaagc aatgaatttt attcattcaa
       61 ataggagagt gagatgtatg gtaacattat ttacttcacc aagttgcaca tcttgccgta
      121 aagcgaaagc atggttacaa gaacatgaca ttccgtatac ggagcgtaat attttttctg
      181 aacatttaac aattgatgaa attaagcaaa tattaaaaat gactgaagac ggtactgatg
      241 aaatcatttc tacacgttct aaaacatacc aaaaattaaa tgttgatatt gattcactac
      301 cattacaaga cttatattca atcattcaag ataatcctgg cttattacgt cgtccaatta
      361 ttttagataa taaacgacta caagttggtt ataatgagga cgagattcga cgtttcttac
      421 ctagaaaagt tcgtacgttc caattacaag aagcacaacg tatggttgac taataaccat
      481 ttaactatgt gtcatacttt actaaaagat atgtcaagca acactatttc tatagtgttg
      541 cttttttggt tttaatttta caataattgc ttagtatctt ttaaatcaaa tgtgcaattg
      601 ttaatatagc tctatattag agtgttttgg atgaaacatt tgctatgata aggtaaaatt
      661 aaattaagaa agtgctttaa tgctttaact gtataaatca tttaaatagt tttgctacaa
      721 cgaatatgtt attataagtt accaaaatat tttagagaaa gaaatcaaac aaataaattg
      781 tattttgttt gatgctttaa tacaatgtgg atactattca tcgactgtaa ggagtgagat
      841 gatatgagaa tagaacgagt agatgataca actgtaaaat tgtttataac atatagcgat
      901 atcgaggccc gtggatttag tcgtgaagat ttatggacaa atcgcaaacg tggcgaagaa
      961 ttcttttggt caatgatgga tgaaattaac gaagaagaag attttgttgt agaaggtcca
     1021 ttatggattc aagtacatgc ctttgaaaaa ggtgtcgaag tcacaatttc taaatctaaa
     1081 aatgaagata tgatgaatat gtctgatgat gatgcaactg atcaatttga tgaacaagtt
     1141 caagaattgt tagctcaaac attagaaggt gaagatcaat tagaagaatt attcgagcaa
     1201 cgaacaaaag aaaaagaagc tcaaggttct aaacgtcaaa agtcttcagc acgtaaaaat
     1261 acaagaacaa tcattgtgaa atttaacgat ttagaagatg ttattaatta tgcatatcat
     1321 agcaatccaa taactacaga gtttgaagat ttgttatata tggttgatgg tacttattat
     1381 tatgctgtac attttgatag tcatgttgat caagaagtca ttaatgatag ttacagtcaa
     1441 ttgcttgaat ttgcatatcc aacagacaga acagaagttt atttaaatga ctatgctaaa
     1501 ataattatga gtcataacgt aacagctcaa gttcgacgtt attttccaga gacaactgaa
     1561 taacgatatt aattaagcaa cgcactatga aaggagactc tttggagtct ctttttttat
     1621 tgatttcaaa agctgaaaaa gtaatagtag ttaaacttac gtgtaatatt ttgaggtgaa
     1681 gatatgttag tagctttaaa tgaagaaaag gaacgtgtgt tagcaactac tgcattgaga
     1741 aagacacaat atttttgtcc tgtgtgtggc aagcaagtta ttttaaagcg tgggctcaaa
     1801 gtaattagtc attttgcaca taaacattta gcggaacaaa aatgttttaa taatgaaacg
     1861 attaaacatt ataaaagtaa attgatttta gcacagatga tacagcaaca aggatgtaaa
     1921 gtagagatag agccattttt aaaagaaata aaacaaattc cggatatttt gattaataat
     1981 aaatatgtta ttgagctaca gtattcgcca attccttata aacagattct tcaacgaacg
     2041 gaaggtttaa agaaaatggg atataaagta agttggttat taaatgatgt tgattattgt
     2101 cataataaag tgaagttcaa tcattttcat agtttgttta ttaatccaat cactcgaaaa
     2161 cttcatacgt tcaatttaga gaaaaaacaa ataatgatgt ttcaacaaat acaatattta
     2221 ggcgggcaca aatatgtcgc tgaaaaaagg aatgccaaaa ttattgagtt gtttaatgag
     2281 gcgccttgtg attatcatgc tgtttataaa ttatcaaagt tcgcaattaa tcaatatatc
     2341 aaatattgtc gctggcaaaa ttctgtttta gaacccactt taagtgcaat gtatcaatta
     2401 cagttaactg atcaagaagt agtgtacaat tatggttata tttttccaga gcaaatttat
     2461 attgaaaatc atccaattga gtggcaatta caagttgatt tatggttaaa gaatggaaaa
     2521 agcaaattag taaatgacaa tcttaattat tttaaactga aaaaatttat tgttgctcta
     2581 gaaagtaaaa cagcaattat agaaaaactt attaacaatt atttaaatat ttgt
//
LOCUS       DANBWH010000262         2600 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.270, whole genome shotgun sequence.
ACCESSION   DANBWH010000262 DANBWH010000000
VERSION     DANBWH010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.270"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            32..2020
                     /gene="tkt"
                     /locus_tag="PPY92_002543"
     CDS             32..2020
                     /gene="tkt"
                     /locus_tag="PPY92_002543"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDJ2626510.1"
                     /translation="MFNEKDQLAVDTLRALSIDTIEKANSGHPGLPMGAAPMAYTLWT
                     RHLNFNPQSKDYFNRDRFVLSAGHGSALLYSLLHVSGSLELEELKQFRQWGSKTPGHP
                     EYRHTDGVEVTTGPLGQGFAMSVGLALAEDHLAGKFNKEGYNVVDHYTYVLASDGDLM
                     EGISHEAASFAGHNKLSKLVVLYDSNDISLDGELNKAFSENTKARFEAYGWNYLLVKD
                     GNDLEEIDKAITTAKSQEGPTIIEVKTTIGFGSPNKAGTNGVHGAPLGEVERKLTFEN
                     YGLDPEKRFNVSEEVYEIFQNTMLKRANEDESQWNSLLEKYAETYPELAEEFKLAISG
                     KLPKNYKDELPRFELGHNGASRADSGTVIQAISKTVPSFFGGSADLAGSNKSNVNDAT
                     DYSSETPEGKNVWFGVREFAMGAAVNGMAAHGGLHPYGATFFVFSDYLKPALRLSSIM
                     GLNATFIFTHDSIAVGEDGPTHEPIEQLAGLRAIPNMNVIRPADGNETRVAWEVALES
                     ESTPTSLVLTRQNLPVLDVPEDVVEEGVRKGAYTVYGSEETPEFLLLASGSEVSLAVE
                     AAKDLEKQGKSVRVVSMPNWNAFEQQSEEYKESVIPSSVTKRVAIEMASPLGWHKYVG
                     TAGKVIAIDGFGASAPGDLVVEKYGFTKENILNQVMSL"
     gene            2298..2540
                     /locus_tag="PPY92_002544"
     CDS             2298..2540
                     /locus_tag="PPY92_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HDJ2626511.1"
                     /translation="MATWLAIIFIVAALILGLIGGFLLARKYMMDYMKKNPPINEEML
                     RMMMMQMGQKPSQKKINQMMTMMNKNMDQNMKSAKK"
     gene            2527..>2600
                     /locus_tag="PPY92_002545"
     CDS             2527..>2600
                     /locus_tag="PPY92_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626512.1"
                     /translation="MRKSKFAIDRGYFPDMEMASFYNQ"
BASE COUNT          908 a          371 c          522 g          799 t
ORIGIN      
        1 tgataaaatt gaagaaggga agatacacaa gatgtttaat gaaaaagatc aattagctgt
       61 tgatacgcta cgtgcactaa gtatcgacac aatcgaaaaa gcgaattctg gtcatccagg
      121 attacctatg ggagctgccc caatggctta cactttgtgg acacgtcatc tgaattttaa
      181 tccacaatct aaagattact tcaatagaga ccgtttcgta ttatcggcag ggcatggttc
      241 agcattattg tatagcttgt tacatgtttc tggtagttta gaattagaag aattaaagca
      301 atttagacaa tggggttcta aaacaccagg ccatcctgaa tacagacata ctgatggtgt
      361 agaagttact actggaccac ttggacaagg ttttgctatg tcagtaggat tagctttagc
      421 agaagatcac ctagcaggga aatttaataa agaaggatat aatgttgtag atcattacac
      481 atatgtatta gcttctgacg gtgatttaat ggaaggtata tcgcatgaag cagcttcatt
      541 tgctggacat aataaattaa gtaaattagt tgttttatac gattcaaatg atatttcatt
      601 agatggcgaa ttaaacaaag ctttttctga aaacacaaaa gctcgttttg aagcatatgg
      661 ttggaattac ttactagtta aagatggtaa tgatttagaa gaaattgata aagcgattac
      721 tacagctaaa tctcaagaag gaccaacgat tattgaagtt aaaacaacaa tcggatttgg
      781 ttcaccgaat aaagcaggaa ctaatggtgt tcatggggca cctttaggtg aagttgaaag
      841 aaaattaaca ttcgaaaatt acggtttaga tcctgaaaaa cgttttaatg tttcagaaga
      901 ggtatacgaa attttccaaa atactatgtt aaaacgtgct aatgaagatg aatctcaatg
      961 gaattcatta ttagaaaaat atgcagaaac atatcctgaa ttagcagaag aatttaaatt
     1021 agcgattagt ggtaaattgc ctaaaaatta taaggatgaa ttaccacgtt ttgaactggg
     1081 tcataatggt gcatctcgtg ctgattctgg tactgttatt caagcaatca gtaaaactgt
     1141 cccttcattc tttggtggat cagcagacct tgctggttca aacaaatcca atgtaaatga
     1201 tgcaacagat tatagttctg aaacacctga aggtaaaaat gtgtggtttg gtgtacgtga
     1261 atttgctatg ggtgctgctg taaatggtat ggctgcacat ggaggtttac atccatatgg
     1321 tgcaacattc ttcgtattta gtgattattt aaaaccagcg ttacgtttat catcaattat
     1381 gggattaaat gcaacgttca tcttcacaca tgattcaatt gcagtaggtg aagatggtcc
     1441 tactcatgaa ccaattgaac aattagctgg attaagagct attccaaata tgaatgttat
     1501 ccgtcctgct gatggtaatg aaacaagagt agcatgggaa gttgccttag aatctgaatc
     1561 tacacctact tcattagtat tgacacgtca aaacttaccg gttttagatg taccagaaga
     1621 tgtagttgaa gaaggcgttc gaaaaggtgc ctatacagtt tatggctctg aagagacacc
     1681 agaattccta ttattagctt caggttcaga agttagtctt gcagttgaag ctgctaaaga
     1741 tcttgaaaaa caaggtaaat cagtgcgtgt tgtttcaatg cctaactgga atgcatttga
     1801 acaacaatct gaagaatata aagaatcagt tattccatca agcgtaacaa aacgtgttgc
     1861 gattgaaatg gcttcaccgc ttggatggca taaatatgta ggtactgcag gtaaagttat
     1921 tgctattgac ggctttggcg caagtgcacc tggcgattta gtagttgaaa aatatggatt
     1981 tactaaagaa aatatcttaa accaagttat gagcttataa gaataattta taaagcgagt
     2041 atgtttagaa gtctaggatg cataatctta ggcttctttt aaagtgttga aatttagagt
     2101 atagcactta aactacatca taagtgataa gttatgaaag tatactattt cagattaatc
     2161 tttaaaagct ctgttataac agcatgattt ttgatattat ttttagtatc gatattaaaa
     2221 tacttgaata aactagttct tgaaataatg tgatgaattt agtaaaattc agtaggataa
     2281 agaaagcggg tgaaacaatg gcaacttggt tagcaattat ttttatagta gctgcattaa
     2341 ttttaggttt aattggaggt ttccttttag ctagaaaata tatgatggac tacatgaaga
     2401 aaaacccacc aatcaacgaa gaaatgcttc gtatgatgat gatgcaaatg ggtcaaaaac
     2461 cttctcagaa gaaaattaat caaatgatga cgatgatgaa taaaaatatg gatcaaaata
     2521 tgaagagtgc gaaaaagtaa attcgcaatt gatagaggct attttccaga tatggaaatg
     2581 gcctcttttt ataatcaaat
//
LOCUS       DANBWH010000263         2552 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.271, whole genome shotgun sequence.
ACCESSION   DANBWH010000263 DANBWH010000000
VERSION     DANBWH010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.271"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            76..543
                     /locus_tag="PPY92_002546"
     CDS             76..543
                     /locus_tag="PPY92_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdC family protein"
                     /protein_id="HDJ2626513.1"
                     /translation="MLYLIFSIIVALFMGTIVIVIRMKAQNYPVNEKKIVLPPFFMAT
                     GALMYVVPYFRLTGSEMLEAFIIGLLFSTVLIWTSRFEVKGTEIYMKRSKAFPVILIS
                     LLIIRTVMKIFISNEIDPGELGGMFFLLAFCMIVPWRAAMLYKYKKLKKTLIN"
     gene            667..1788
                     /locus_tag="PPY92_002547"
     CDS             667..1788
                     /locus_tag="PPY92_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDJ2626514.1"
                     /translation="MKIIHTADWHLGKILNGKQLLEDQAYILDMFVEKMKEEEPDIIV
                     IAGDLYDTTYPSKDAIMLLEQAIGKLNLELRIPIIMISGNHDGKERLNYGASWFEHNQ
                     LFIRTDFTSINSPIEINGVNFYTLPYATVSEMKHYFEDDTIETHQQGITRCIETIAPE
                     IDEDAVNILISHLTVQGGKTSDSERPLTIGTVESVQKGVFDIFDYVMLGHLHHPFSIE
                     DDKIKYSGSLLQYSFSEAGQAKGYRRLTINDGIINDVFIPLKPLRQLEIISGEYNDVI
                     NEKVHVKNKDNYLHFKLKNMSHITDPMMSLKQIYPNTLALTNETFNYNEENNAIEISE
                     KDDMSIIEMFYKHITDKELSDIQSKKIKNILENELRKED"
     gene            1792..>2552
                     /locus_tag="PPY92_002548"
     CDS             1792..>2552
                     /locus_tag="PPY92_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDJ2626515.1"
                     /translation="MKPLHLKLNNFGPFLKEEIDFSKIDNNELFLISGKTGSGKTMIF
                     DAMTYALFGKASTEQREENDLRSHFADGKQPMSVTFEFQLNHRIYKVHRQGPYIKEGN
                     TTKTNAKFDVFEMVDGKYEIRESKVISGTQFIIELLGVNADQFRQLFILPQGEFKRFL
                     ISNSREKQGILRTLFDSEKFEAIREILKEELKKEKAQIENRYQQIDLLWQEIESFDDD
                     KIKGLLELATQQIDKLIENIPLLQARSKEILAFVN"
BASE COUNT          967 a          324 c          458 g          803 t
ORIGIN      
        1 gacgaataga ctgagaaatg ctataattca ttttgtatga tttacagaga gtttatttaa
       61 cgagaaggtg tctgcgtgct ctatttaata ttttcaatca ttgtagcttt atttatggga
      121 actatagtta tagttattcg tatgaaagct caaaattatc cggtaaatga gaaaaaaata
      181 gttttgccac cgttttttat ggcgaccggt gcattgatgt acgtcgttcc atattttagg
      241 ctaacaggat cggaaatgct agaagccttt ataattggtt tgcttttttc tacagttcta
      301 atttggactt ctcgatttga agtcaaaggt acagaaattt atatgaaacg atctaaagca
      361 tttccagtta ttttgatttc attacttatc attcgtactg tgatgaaaat attcattagt
      421 aatgaaatag atcctggaga attaggcggc atgttctttt tattagcatt ctgtatgatt
      481 gttccttgga gagcagcaat gctatataaa tacaaaaaac taaagaaaac attaatcaat
      541 taattacttt taaaaccact tgtgatcgac ttctaaatca gtcaatgagt ggttttaatt
      601 ttacttgaaa aggtgaaatg attatattaa aataaaagcg aacaaatgtt ctatatggag
      661 gcagtaatga aaattataca tacagcagac tggcacttag ggaaaatatt aaatggcaaa
      721 cagcttttag aagatcaagc gtatatttta gatatgttcg tagaaaaaat gaaagaagaa
      781 gaacctgata tcattgtgat agctggagat ttatatgaca caacatatcc aagtaaagat
      841 gcaatcatgt tattagaaca agcgattgga aagttaaatt tagaactgcg tataccaata
      901 attatgatta gtggaaatca cgatggtaaa gagaggttaa actatggggc gagttggttt
      961 gaacataatc agttatttat aagaacagat tttacatcga ttaattcacc aatagagata
     1021 aatggggtta atttttatac actcccttat gctactgtga gcgaaatgaa acactacttt
     1081 gaagatgaca ccattgaaac acatcaacag ggaattacgc gctgtattga aacaatagca
     1141 ccggaaattg atgaagatgc cgtcaatatt ttaattagtc atctgactgt tcaaggtgga
     1201 aagacatctg attctgaaag accattaact attggaacgg ttgaatcagt tcagaaaggt
     1261 gtttttgata tatttgatta tgtcatgcta ggtcacttgc atcatccatt tagtatagaa
     1321 gacgacaaaa ttaaatatag tggctcctta ttgcagtatt cattttcgga agcgggtcaa
     1381 gctaaagggt atagacgttt aacaattaat gatggcatta ttaacgatgt atttattcct
     1441 cttaagccac ttagacaatt ggaaattatc tcaggcgaat ataatgatgt tattaatgaa
     1501 aaagttcatg tgaaaaataa agataattat ttacatttta aacttaaaaa tatgtctcat
     1561 attactgatc caatgatgag tttaaaacaa atttatccta atactttagc gctgacgaat
     1621 gaaactttta attataatga agaaaataat gctatagaaa taagtgaaaa agatgacatg
     1681 tcaattatcg aaatgtttta taaacatata actgataaag aattatcgga tatccaatct
     1741 aaaaagataa aaaatatttt agaaaacgaa ttgagaaagg aggattaatg aatgaaacca
     1801 ttacatttaa agttgaataa tttcggcccc tttttaaaag aagaaattga tttttctaaa
     1861 attgataata atgaattgtt tttaataagt ggtaagactg gatcgggtaa aacaatgatt
     1921 tttgatgcaa tgacttatgc cttgtttggt aaagcatcaa ctgaacaaag agaagaaaat
     1981 gatttgagaa gtcatttcgc tgatggtaaa cagccgatgt cagtaacatt tgaatttcaa
     2041 ttaaatcatc gaatttataa agtgcataga caaggccctt atatcaaaga aggtaataca
     2101 acaaaaacga acgctaaatt tgatgtattt gagatggtgg atggcaagta tgaaattaga
     2161 gaaagtaaag taatttcagg tacccaattc attattgaat tattaggagt aaatgcagat
     2221 caattccgac aattgtttat tttgcctcaa ggtgaattca aacgcttttt aatatcaaac
     2281 agtcgtgaaa agcaagggat attaagaaca ctgtttgaca gtgaaaaatt tgaagctata
     2341 cgagaaatat taaaagaaga actaaaaaaa gaaaaagctc aaatcgagaa tagatatcaa
     2401 caaattgacc ttttatggca agaaattgaa tcatttgatg atgacaaaat aaaaggctta
     2461 ttagagcttg ccactcagca gatagacaaa ttgattgaaa atataccact tttacaagct
     2521 aggtcgaaag aaatactagc atttgtaaat ga
//
LOCUS       DANBWH010000264         2543 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.272, whole genome shotgun sequence.
ACCESSION   DANBWH010000264 DANBWH010000000
VERSION     DANBWH010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.272"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(90..173)
                     /locus_tag="PPY92_002549"
     tRNA            complement(90..173)
                     /locus_tag="PPY92_002549"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(137..139),aa:Leu,seq:caa)
     gene            complement(212..286)
                     /locus_tag="PPY92_002550"
     tRNA            complement(212..286)
                     /locus_tag="PPY92_002550"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(251..253),aa:Gly,seq:tcc)
     gene            complement(390..464)
                     /locus_tag="PPY92_002551"
     tRNA            complement(390..464)
                     /locus_tag="PPY92_002551"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(429..431),aa:Gly,seq:tcc)
     gene            complement(472..545)
                     /locus_tag="PPY92_002552"
     tRNA            complement(472..545)
                     /locus_tag="PPY92_002552"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(511..513),aa:Cys,seq:gca)
     gene            complement(551..622)
                     /locus_tag="PPY92_002553"
     tRNA            complement(551..622)
                     /locus_tag="PPY92_002553"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(588..590),aa:Gln,seq:ttg)
     gene            complement(634..706)
                     /locus_tag="PPY92_002554"
     tRNA            complement(634..706)
                     /locus_tag="PPY92_002554"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(671..673),aa:His,seq:gtg)
     gene            complement(709..782)
                     /locus_tag="PPY92_002555"
     tRNA            complement(709..782)
                     /locus_tag="PPY92_002555"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(748..750),aa:Trp,seq:cca)
     gene            complement(798..878)
                     /locus_tag="PPY92_002556"
     tRNA            complement(798..878)
                     /locus_tag="PPY92_002556"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(842..844),aa:Tyr,seq:gta)
     gene            complement(884..959)
                     /locus_tag="PPY92_002557"
     tRNA            complement(884..959)
                     /locus_tag="PPY92_002557"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(924..926),aa:Thr,seq:tgt)
     gene            complement(967..1039)
                     /locus_tag="PPY92_002558"
     tRNA            complement(967..1039)
                     /locus_tag="PPY92_002558"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1004..1006),aa:Phe,seq:gaa)
     gene            complement(1058..1133)
                     /locus_tag="PPY92_002559"
     tRNA            complement(1058..1133)
                     /locus_tag="PPY92_002559"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1098..1100),aa:Asp,seq:gtc)
     gene            complement(1155..1228)
                     /locus_tag="PPY92_002560"
     tRNA            complement(1155..1228)
                     /locus_tag="PPY92_002560"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1192..1194),aa:Met,seq:cat)
     gene            complement(1243..1335)
                     /locus_tag="PPY92_002561"
     tRNA            complement(1243..1335)
                     /locus_tag="PPY92_002561"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1297..1299),aa:Ser,seq:tga)
     gene            complement(1374..1449)
                     /locus_tag="PPY92_002562"
     tRNA            complement(1374..1449)
                     /locus_tag="PPY92_002562"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1414..1416),aa:Asp,seq:gtc)
     gene            complement(1460..1549)
                     /locus_tag="PPY92_002563"
     tRNA            complement(1460..1549)
                     /locus_tag="PPY92_002563"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1511..1513),aa:Ser,seq:tga)
     gene            complement(1559..1632)
                     /locus_tag="PPY92_002564"
     tRNA            complement(1559..1632)
                     /locus_tag="PPY92_002564"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1596..1598),aa:Ile,seq:cat)
     gene            complement(1661..1734)
                     /locus_tag="PPY92_002565"
     tRNA            complement(1661..1734)
                     /locus_tag="PPY92_002565"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1698..1700),aa:Met,seq:cat)
     gene            complement(1756..1831)
                     /locus_tag="PPY92_002566"
     tRNA            complement(1756..1831)
                     /locus_tag="PPY92_002566"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1796..1798),aa:Ala,seq:tgc)
     gene            complement(1849..1922)
                     /locus_tag="PPY92_002567"
     tRNA            complement(1849..1922)
                     /locus_tag="PPY92_002567"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1886..1888),aa:Pro,seq:tgg)
     gene            complement(1932..2005)
                     /locus_tag="PPY92_002568"
     tRNA            complement(1932..2005)
                     /locus_tag="PPY92_002568"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1969..1971),aa:Arg,seq:acg)
     gene            complement(2024..2112)
                     /locus_tag="PPY92_002569"
     tRNA            complement(2024..2112)
                     /locus_tag="PPY92_002569"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2076..2078),aa:Leu,seq:taa)
     gene            complement(2123..2197)
                     /locus_tag="PPY92_002570"
     tRNA            complement(2123..2197)
                     /locus_tag="PPY92_002570"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2163..2165),aa:Gly,seq:gcc)
     gene            complement(2201..2282)
                     /locus_tag="PPY92_002571"
     tRNA            complement(2201..2282)
                     /locus_tag="PPY92_002571"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2246..2248),aa:Leu,seq:tag)
     gene            complement(2286..2361)
                     /locus_tag="PPY92_002572"
     tRNA            complement(2286..2361)
                     /locus_tag="PPY92_002572"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2326..2328),aa:Lys,seq:ttt)
     gene            complement(2367..2442)
                     /locus_tag="PPY92_002573"
     tRNA            complement(2367..2442)
                     /locus_tag="PPY92_002573"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2407..2409),aa:Thr,seq:tgt)
     gene            complement(2459..2534)
                     /locus_tag="PPY92_002574"
     tRNA            complement(2459..2534)
                     /locus_tag="PPY92_002574"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2499..2501),aa:Val,seq:tac)
BASE COUNT          709 a          647 c          600 g          587 t
ORIGIN      
        1 ttttatttgt attgtataga gagaaataaa aagaaacctt gttttacaag gtttctaata
       61 ggttatgtta tgtaaataac agttaattat accggtggtc ggggtcgaac cgacactcca
      121 caagtggaac gggattttga gtcccgcgcg tctgccaatt ccgccacacc ggcttaatgg
      181 taaacaaaaa acttcccttt ggaagcaatt atggagcgga agataggatt tacacctata
      241 cctcgttccg ggaaggaacg tgttctaaaa gttgaactac tcccgcaaat attaaattat
      301 ggagcggaag ataggattta cacctatacc tcattccagg aaggaatgta ttctaagagt
      361 tgaaatactc ccgcattatt attaaattat ggagcggaag ataggatttg cacctatacc
      421 tcgttccggg aaggaacgtg ttctaaaagt tgaactactc ccgcataaac ctggaggcgg
      481 caaccggatt tgaaccggtg ataaaggttt tgcagacctc tgccttacca cttggctatg
      541 ccgccaataa ctgggctagc tggattcgaa ccaacgagtg acggagtcaa agtccgttgc
      601 cttaccgctt ggctatagcc cattaataat aagggcggct gaaggggatc gaaccctcga
      661 atgtcggaac cacaatccga tgtgttaacc acttcaccac agccgccatg gcaggggcag
      721 taggaatcga acccacacca aaggttttgg agacctctat tctaccgttg aactatgccc
      781 ctattaaaaa taataaatgg aggggggcag attcgaactg ccgaacccga aggagcggat
      841 ttacagtccg ccgcgtttag ccacttcgct acccctccat aaatggtgcc ggccagagga
      901 cttgaacccc caacctactg attacaagtc agttgctcta ccaattgagc taggccggct
      961 aagaaatggt tcaggacaga gtcgaactgc cgacacatgg agcttcaatc cattgctcta
     1021 ccaactgagc tactgaacca taataaaaat gtaatgatgg cggtctcgac gggaatcgaa
     1081 cccgcgatct cctgcgtgac aggcaggcgt gttaaccgct acactacgag acctattaaa
     1141 ttaaaaacta tgtattgcgg gaggcggatt tgaaccaccg accttcgggt tatgagcccg
     1201 acgagctacc gaactgctcc atcccgcgat aataaaaaat aatggcggag gaagagggat
     1261 tcgaaccccc gcggcccgtt aaggccctgt cggttttcaa gaccgatccc ttcagccgga
     1321 cttgggtatt cctccattat tataggtaaa tcgctattaa ttataaaatt aaatggcggt
     1381 ctcgacggga atcgaacccg cgatctcctg cgtgacaggc aggcgtgtta accgctacac
     1441 tacgagacca ttagtaaaac ggaggaagag ggattcgaac ccccgcgagc cgttaagccc
     1501 ctgtcggttt tcaagaccga tcccttcagc cggacttggg tattcctcca aaattatatg
     1561 gaccttgcag gactcgaacc tgcgaccgaa cggttatgag ccgttagctc taaccaactg
     1621 agctaaaggt cctaaatata attttacaac taataaatag tggcggtgga ggggatcgaa
     1681 cccccgacct cacgggtatg aaccgtacgc tctagccagc tgagctacac cgccttatat
     1741 agtttgtaaa taatatggtg gagactagcg ggatcgaacc gctgacctcc tgcgtgcaaa
     1801 gcaggcgctc tcccagctga gctaagcccc cataataatt acagtatatc gggaagacag
     1861 gattcgaacc tgcgacccct tggtcccaaa ccaagtgctc taccaagctg agctacttcc
     1921 cgtataatta acgcgcccga taggagtcga acccataacc tcttgatccg tagtcaaacg
     1981 ctctatccaa ttgagctacg ggcgcatatg tttttattga aaatggtgcc gaggaccgga
     2041 atcgaaccgg tacggtgatc actcaccgca ggattttaag tcctgtgcgt ctgccagttc
     2101 cgccaccccg gcactataaa aatggagcag aagacgggat tcgaacccgc gaccccaacc
     2161 ttggcaaggt tgtattctac cgctgaacta cttctgcata tgcgggtgaa gggagtcgaa
     2221 cccccacgcc gtaaggcgct agatcctaag tctagtgcgt ctgccaattc cgccacaccc
     2281 gcaaatggtg agccatagag gattcgaacc tctgaccctc tgattaaaag tcagatgctc
     2341 taccaactga gctaatggct cttccatggt gccggccaga ggacttgaac ccccaaccta
     2401 ctgattacaa gtcagttgct ctaccaattg agctaggccg gcaatatgta agaataaatg
     2461 gtggagaatg acgggttcga accgccgacc ctctgcttgt aaggcagatg ctctcccagc
     2521 tgagctaatt ctccgattta aaa
//
LOCUS       DANBWH010000265         2542 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.273, whole genome shotgun sequence.
ACCESSION   DANBWH010000265 DANBWH010000000
VERSION     DANBWH010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.273"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..982
                     /locus_tag="PPY92_002575"
     CDS             <1..982
                     /locus_tag="PPY92_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDJ2626516.1"
                     /translation="FDNARAILILLVVFGHMLQPYTSGDKYLSALYLVIYSFHMPTFL
                     FISGYFAKNIDKPYYLEKISKRLIVPYMIFFAFFSIYYFLTGKSDELQLDPFNPVFAL
                     WFLITLFFFHVILVIVRRFNPYKVLSVSIIISIGAGFSDNIDSYLSISRTIVFFPIFY
                     LGYIFTKKHTAIFKNKKLIPVSIITFILFFIIYVIHPINADWLLGSSPYTSLENEGQS
                     IFSPFKRLILYGIILIAMTAFLNLMSTKKKLYTYIGSRTLYVYLLHGLIIGIVRGFEW
                     YPFDNPISLMTYLYLISISVIIVYVLSTNFVCKWTNPIINLQRPSQFKDS"
     gene            1178..2332
                     /locus_tag="PPY92_002576"
     CDS             1178..2332
                     /locus_tag="PPY92_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDJ2626517.1"
                     /translation="MKLSLNSNSKYLRAPSIRQFSNRMNNLDDCVNLTIGQPDFPMPD
                     VVKKAYIDAINNDKTSYSHNKGLLETREAISQYFKNRYHFSYDPEEIIVTNGASEAID
                     TTLRSIIEPGDEIIIPGPIYAGYIPLIEVLGGKPIYIDTTATQFKITPDALESHISPK
                     TKAVLLNYPTNPTGVVLKRNEVLNIVNVLKKYPIFIISDEIYAENTFSGKHVSFAEFE
                     DIRDQLILIGGLSKSHSATGIRIGFLLGPQYLIDKLTFMHAYNCICANVPAQIACITA
                     LNEGLEAPKYMNEAYVERRNYLVSELTKLGFEITAQPEGAFYIFPSIKHITDDDFEFC
                     VDLLESTHLAIVPGSSFTEFGKGFVRISYAYEMDVLKEGMKRLAKYLNTK"
BASE COUNT          878 a          382 c          361 g          921 t
ORIGIN      
        1 ttttgacaac gcaagagcaa ttttaatatt attagtcgta tttggacata tgttacaacc
       61 ctacactagt ggcgataaat atttatcagc attataccta gttatttata gtttccatat
      121 gccaacattt ttgtttatat ctggttattt cgcgaaaaat attgataaac catactattt
      181 agaaaaaatt tcaaaacgtt tgatagtacc ttatatgata ttttttgcat tcttttcaat
      241 atattatttc ttaacaggta aaagtgacga attacaactt gatcctttta acccagtatt
      301 tgcgttatgg tttttgatca cgctattttt ctttcatgtc attttagtaa tagttagaag
      361 atttaatcca tataaagtgc tatcagtttc aattattata tctattggag caggtttttc
      421 agataacatc gatagctact tgagcatttc aaggacaatc gtatttttcc caatatttta
      481 cctaggttac attttcacca aaaaacatac ggctattttt aaaaataaaa aactaatacc
      541 agtatctatc attaccttta ttttgttttt tatcatttac gtgattcatc ctatcaatgc
      601 agattggtta ttgggaagtt caccatatac ttcacttgaa aatgaaggac agagtatatt
      661 cagtcctttt aaacgactta tattatatgg aattatatta atcgcaatga ctgcgtttct
      721 aaatttaatg tcaacaaaga aaaaattata tacttatatt ggtagtcgta cgctatacgt
      781 atatttatta catggtttaa ttattggcat tgttagagga tttgaatggt acccattcga
      841 taatcctata tcattaatga cttatcttta tttaataagt atatctgtaa tcatcgtata
      901 tgtattatct acaaactttg tttgtaaatg gacaaatcca ataattaatt tacaaagacc
      961 atcacagttt aaagattcat aattttatag tgtctaagta cattaactgt aaccacttta
     1021 ttcattttga aaagtagtta ctacagtgta tttagacatt tttcgttttt caaataaagc
     1081 ttaagtaatt tacccttttt tggtttaaaa gtatgttatg atattattaa tttctaaatt
     1141 tttagacaat atttgattta tatttgaggt gtcacaaatg aaactttctt taaattctaa
     1201 ttctaaatat ttaagagcac caagtattcg ccaattttca aatcgtatga ataatttgga
     1261 tgattgtgtt aatttaacta ttggccaacc agacttcccg atgcctgatg ttgtgaaaaa
     1321 ggcatatatt gatgctatta acaatgacaa gacatcatac tctcacaata aagggttatt
     1381 agaaactcgc gaagcaatta gtcaatactt caaaaatcgt tatcattttt cctatgatcc
     1441 tgaagaaatt attgtgacaa atggagcaag tgaagcaata gacacaacgt tacgttctat
     1501 catagagcct ggagatgaaa ttataatacc gggaccaatt tatgcaggct acataccact
     1561 catcgaagta ctaggtggta aaccaattta tattgataca acagcaacac aattcaaaat
     1621 tacacctgat gcattagaaa gtcatatttc tccaaagaca aaagctgtct tgttaaatta
     1681 tccgactaat ccaactggcg tagttttaaa aagaaatgaa gttttaaata tcgtaaatgt
     1741 attaaaaaaa tatccgatat ttattattag cgatgagatt tatgctgaaa atacatttag
     1801 tggtaaacat gtatccttcg ctgaatttga agacattcgt gatcaattaa tattaattgg
     1861 tggtttaagt aaatcacact cagcaaccgg aataagaatt ggttttctat tagggcctca
     1921 atatttgatt gataaattaa catttatgca cgcctataat tgtatttgtg ccaatgttcc
     1981 agctcaaata gcatgtataa cagcacttaa cgaaggatta gaagctccta aatatatgaa
     2041 cgaagcttat gtagaacgaa gaaattattt agtatctgaa ttaactaaat taggttttga
     2101 gattaccgct caacctgaag gcgcgtttta tattttccca agtattaaac atatcaccga
     2161 tgacgatttc gaattttgtg tcgatttact tgaatcaaca catttagcaa tcgttccagg
     2221 ctcatccttt acagaattcg gtaaaggatt tgtaaggatt tcctatgctt atgaaatgga
     2281 tgttttaaaa gaaggtatga agagacttgc taaatattta aatactaaat aaaaatgata
     2341 tgttgaactg gacgtcgtga actaaattca tagtacgttc agttttttat attctataag
     2401 ttttaaataa tactctatag agactgtaaa tactaccgag ttatattcct ctcctttaaa
     2461 cacctcactc aaaatgattc tatcaaaagg ttttaatatg ctaacttata attgttcttc
     2521 gaaacttaag cactcaaata at
//
LOCUS       DANBWH010000266         2529 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.274, whole genome shotgun sequence.
ACCESSION   DANBWH010000266 DANBWH010000000
VERSION     DANBWH010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.274"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..329
                     /locus_tag="PPY92_002577"
     CDS             <1..329
                     /locus_tag="PPY92_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDJ2626518.1"
                     /translation="IIKKFGDHNLTFAIEFCSKTIANNTKRHKSVSKLSSQPVFKYGY
                     NDLNELDKVLPNKMKVINEYNYFDYHKKRWGLFGYCRYIPYLKKRLNNKIVIMEYKPA
                     KLITHN"
     gene            complement(1227..>2529)
                     /locus_tag="PPY92_002578"
     CDS             complement(1227..>2529)
                     /locus_tag="PPY92_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDJ2626519.1"
                     /translation="KVHKPLYFYLMLFFSTTIIGALLLYLPFTGKKPISFLDALFIAS
                     SAFTVTGLSPVDIGSQFNILGEIVILLLIQIGGLGIVTVTLLTLVFLNRKISMKNRFL
                     IMVTWNIDEPGGVIKLIKHLAIYSLVTELIGMICLCLSFIPKFGIGKGLFLSLFTSVS
                     AFNNAGFALFKNNLIDYSSDPIVIITISILIIFGGIGHFVVIDFINCKKLSKLSLHSK
                     LVLTTTSILIIIGAITFFLLEQFNTMQHMGLVEKIGNSFFQSVTTRTAGFNSIDIASI
                     NKSTALMLMLLMFIGGAPLSAAGGIKITTFAVAFIFVLNYIRKENNVSVFNKEISDKH
                     IKLSIVTINISFLFISIITFILSIINPNISLIKLLFEVVSAFGTVGLSMNLTTEYHGI
                     TKIIIIFVMLCGKVGLLTLLRTFIPPKSPKNYRYTKGQIYL"
BASE COUNT          965 a          316 c          385 g          863 t
ORIGIN      
        1 ctattattaa aaagtttgga gatcataatt tgacatttgc gattgaattt tgctcaaaaa
       61 caattgcaaa taatacaaaa agacataaat ctgtatcaaa attatcctca caacctgttt
      121 ttaaatatgg atacaatgat ttaaatgaat tggataaagt tttaccaaat aaaatgaaag
      181 ttataaatga atataattac tttgactacc ataagaaaag gtgggggctc tttgggtatt
      241 gtagatatat accttatctg aaaaaaaggc taaacaataa aatagtaatt atggaatata
      301 aaccagctaa attaatcact cataattgag tattaaagta aaatatttca aaaaaacgta
      361 gataggaatt tattaaaagt aaagtgcaca aatatcttgt gttttttcaa ttttaacatt
      421 ttacattaac tttattaggt gatataagat gctgagagaa tcattatatt gcaatgaaaa
      481 atcatcatat ggataatcat atcgatcatt gcaaatatac ttatagagat ttatgtgtgt
      541 aataattggt ggtcataaat tggtcataat gaaataaaaa aactaaaaaa attgaatgta
      601 taaagaatac acgatactga tttaatagta tttttgtatg tgatttatat ctatttcata
      661 ccgcccttaa tgccgggaat gatgtaaaac agtttctagt tttgactaac tagaacattc
      721 catgatagac gagtatgatt cgttccaaat aaatattaga gcgtatgaaa atattttaat
      781 ttaagacacc ttccattagt tgactaaact tatgagaggc gtctttttta tgtgctaatt
      841 tgtaattgag attcataatg gtatttaaat cattatggtg cgggagttat ggtattgcca
      901 taatatgaga gtgcatccac ttctataagt aatgcatatt gcgagtgcag gaatgaatga
      961 gtgcttgatt aaaatcctta tgggtggttg acataattaa aagaaaccac atttaaaatt
     1021 tcttaatcac aagcggttaa ctaggtatag tttagttttg agtaaatctt tttaaaggtg
     1081 tacttgtgca ttttacttaa ttaaagagat aagacattta atgggcctaa aataaataaa
     1141 aaacaaaaaa ctacctgttt aggtagtttt ttaaatgtaa tagattaaaa cactagttca
     1201 tttcttgtta aagatggata gttattttat agataaattt gtcctttagt gtagcggtaa
     1261 tttttaggac tttttggtgg tataaatgtt cttaataaag ttaatagtcc tactttaccg
     1321 caaagcataa cgaatataat aattatttta gtaataccat gatattctgt ggtaaggttc
     1381 atacttaacc cgactgttcc gaatgcagaa accacttcga ataataactt gattaatgat
     1441 atgttcggat taattatcga taatataaaa gtaatgatac tgataaatag aaatgagata
     1501 ttaatggtaa caatagatag ttttatatgt ttgtcagata tttctttatt gaatactgaa
     1561 acattatttt ctttacgtat ataatttaaa acaaatataa acgccactgc aaaagtagtt
     1621 attttaattc ctccagctgc actgagaggg gcaccaccaa taaacataag taacattaac
     1681 attaaggcgg tagatttgtt aatgcttgct atatcaatac tgttaaaacc cgctgttcgt
     1741 gttgttactg attggaaaaa agaatttccg attttttcaa ctagtcccat atgttgcata
     1801 gtattaaact gttctaataa aaagaatgta atagctccta taattattag gatactagtt
     1861 gtagttaaga ctaatttaga atgtaaagat aatttactca attttttaca attaataaag
     1921 tctattacga caaaatgtcc aatacctcca aatattatga gtattgagat tgtaataatg
     1981 acaattggat cactagaata atctattaag ttattcttaa aaagggcaaa tccagcatta
     2041 ttaaaagctg atactgatgt gaataagctt aaaaataaac ctttgcctat accaaatttt
     2101 ggtataaaag ataaacacaa acaaatcata ccaattaatt cagtgactaa actataaata
     2161 gccaagtgtt taattagctt aataacacca ccaggttcgt caatattcca tgtaaccata
     2221 atcaagaatc tatttttcat tgatatcttt ctatttaaaa atactagtgt caatagggtt
     2281 acggtcacga tacccagacc acctatttga attaataata gtattactat ttcaccaagt
     2341 atattaaact gtgatcctat atcaactggg gataggccag taactgtaaa tgcacttgaa
     2401 gctataaata gggcatctaa aaaagatatt ggctttttac cagtgaaagg taaatataat
     2461 aaaagagcac ctatgatagt tgtagagaag aaaagcatta aataaaaata taaaggtttg
     2521 tggactttg
//
LOCUS       DANBWH010000267         2522 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.275, whole genome shotgun sequence.
ACCESSION   DANBWH010000267 DANBWH010000000
VERSION     DANBWH010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.275"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            72..548
                     /locus_tag="PPY92_002579"
     CDS             72..548
                     /locus_tag="PPY92_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDJ2626520.1"
                     /translation="MFKVRQATEKDVVQIRDVATKAWFNTYLNIYAATTVNHLLEASY
                     NEHHLKKRLQEQLFLVVEEGNDIVGFANFIYGEELYLSAHYVKPESQHTGYGTALLNE
                     GLSRFEDKFEGVYLEVDNKNEEAVAYYKEQGFTILRSYQPEMYGEKLDLALMYKAF"
     gene            588..1883
                     /locus_tag="PPY92_002580"
     CDS             588..1883
                     /locus_tag="PPY92_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDJ2626521.1"
                     /translation="MTNAYVDLKLVEEKVFKDPIHRYIHVEDQLIWDLIKTKEFQRLR
                     RIRQLGTLYLSFHTAEHSRFGHSLGVYEIVRRLIDESFIGHDAWDNKDRPLALCAALL
                     HDLGHGPFSHSFEKIFNTDHEAYTQAIITGDTEVNAVLRKVSPEFPREVAEVINKTHH
                     NKLVISMISSQIDADRMDYLQRDAYFTGVSYGAFDMERILRLMRPSKDEVLIKESGMH
                     AVENFIMSRYQMYWQIYFHPVSRGGEVLLNNCLKRAKQLYNEGYEFKLHPHDFIPFFE
                     ETVTIEQYVELDEAVVTYYLEKWTKEDDAILSDLASRFINRDLFKYIPFDGSIITISE
                     LQELFEAGGINPDYYFVSEAFSDLPYDYDRPGSNRKPIHLLRQDGTIREISNQSLVIH
                     SITGINRQDYKLYYPREMVAKIKDKTIREAIENLINELN"
     gene            1911..2432
                     /locus_tag="PPY92_002581"
     CDS             1911..2432
                     /locus_tag="PPY92_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwhD family protein"
                     /protein_id="HDJ2626522.1"
                     /translation="MEVISLSEKKGFNFNIIKNDPLDGHKGTNIGSISLDNIAPVFID
                     VANKEAFIDIGGMHARAKVEKGVKWITDKAAVEGDEAKEYWLCWVTTERNEQGPYYAG
                     LTACYLLVNKAIRRGYKSMPEHVNMMDKSMKHHIIIDQIGDENKAILKDFLMNHDEGM
                     WKLSSDALHQAFN"
BASE COUNT          906 a          325 c          489 g          802 t
ORIGIN      
        1 ttgtttatta agtattgagc aagttgtggt ataatgccgt tattaaaact tgttaataag
       61 gggagaaagt catgttcaag gtaagacaag caactgaaaa agatgttgtt caaattagag
      121 atgtcgcaac taaagcttgg tttaatacat acttaaatat atacgctgcg acaacagtta
      181 atcacttgtt agaagcttca tataatgaac atcatttaaa gaaaagactt caagaacaat
      241 tattcttagt cgttgaagaa ggtaatgaca tcgttggctt tgctaacttt atttacggtg
      301 aagaattata tttatcagct cattatgtta aaccagaatc gcaacataca ggttatggta
      361 cagcattgtt aaatgaagga ttatcacgtt ttgaagataa atttgaaggt gtttacttag
      421 aagtagataa taaaaatgaa gaagcagtag cttactataa agagcaaggt tttacaatct
      481 tacgctctta tcagccagaa atgtatggcg aaaagttaga cttagcactt atgtacaaag
      541 cattttaagt aaattaaata ttatattaaa cgaaaaggag gggaataatg actaacgcat
      601 atgtagattt aaaattagta gaagaaaaag tttttaaaga tccgatacat cgatatattc
      661 atgttgaaga tcaattgata tgggatttaa ttaaaactaa ggaattccaa aggttacgtc
      721 gaattagaca actaggaaca ctgtacctat cttttcacac agcagaacat agtcgctttg
      781 gacattcttt aggtgtgtat gaaatagtta gacgattaat tgatgagtca tttattggtc
      841 atgatgcatg ggacaataaa gatagaccgt tggcattatg tgctgcatta ttacatgatt
      901 taggacatgg tccattttca catagttttg aaaaaatatt taatacagac catgaagcat
      961 acacacaagc gattattact ggagatactg aggtgaatgc tgtattacgt aaagtgtcgc
     1021 ctgagtttcc aagagaagtt gcggaagtaa ttaataaaac gcatcataat aaattggtca
     1081 tttcgatgat ttcgtcacaa atcgatgcgg atagaatgga ttatttacaa cgtgatgcgt
     1141 atttcacagg tgtatcatat ggtgcttttg atatggagcg tattttaaga ttaatgcgac
     1201 cttctaaaga tgaagtacta atcaaagaaa gtggtatgca tgcagttgaa aactttatta
     1261 tgagtcgtta tcaaatgtat tggcaaattt acttccaccc agttagtcgt ggtggagaag
     1321 tgctgcttaa taattgtttg aaacgcgcaa aacagcttta taatgaaggc tatgaattta
     1381 agttgcatcc acatgatttt attccatttt ttgaagagac agttacgatt gaacaatatg
     1441 ttgaactcga tgaagcggta gttacgtatt atttggaaaa atggacaaaa gaagatgatg
     1501 ccattttaag tgatttagca agtcgattta ttaatcgaga cttatttaaa tatattccat
     1561 ttgatggctc aattattaca atatcagaac tgcaagaact gtttgaagca ggtggtatta
     1621 atccagatta ttattttgtg agtgaagcat tttcggattt gccatatgac tatgatcgac
     1681 cggggtcaaa tcgcaaaccg attcatttat taagacaaga tggtacgatt agagaaataa
     1741 gcaatcaatc attagtcatt catagtatta caggcattaa tcgccaagac tataaattat
     1801 attatcctag agaaatggtt gcaaagatta aagataagac aattagagaa gctattgaaa
     1861 atttgattaa tgagcttaat taaacagggc taaaattgtt atcgttaaat atggaggtta
     1921 tatcattgtc tgagaaaaaa ggctttaatt ttaatatcat aaaaaatgac cctctagatg
     1981 gtcataaagg tacaaatatt ggttcaatta gcttagacaa tattgcacca gtttttatcg
     2041 atgttgctaa caaagaagca tttattgata ttggaggcat gcatgctcgt gccaaagttg
     2101 aaaaaggtgt gaaatggatt actgataaag ctgctgttga aggcgatgaa gctaaagaat
     2161 attggttgtg ttgggtaaca acagaacgta atgaacaagg accatattac gctggtttaa
     2221 cagcgtgcta tttattagtg aataaagcaa ttcgtcgtgg ttataaaagt atgcctgaac
     2281 atgttaatat gatggataaa tcaatgaaac atcatattat cattgatcaa attggtgacg
     2341 agaataaagc tattttaaaa gactttttaa tgaaccatga tgaaggtatg tggaagcttt
     2401 cttctgatgc tttacatcaa gcatttaatt aaatattaga aactaaaatt tcccaattaa
     2461 tctataaaga tatgattcat ttctcaatga cgacgtaaat cttgaggtga aaataaagtc
     2521 tt
//
LOCUS       DANBWH010000268         2472 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.277, whole genome shotgun sequence.
ACCESSION   DANBWH010000268 DANBWH010000000
VERSION     DANBWH010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.277"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            76..1644
                     /locus_tag="PPY92_002582"
     CDS             76..1644
                     /locus_tag="PPY92_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDJ2626523.1"
                     /translation="MIEKLVTFLNEVVWSKPLVYGLLITGVLFTLRMRFFQVRHFKEM
                     IRLMFQGEKSPNGISSFQAIAMSLAGRVGTGNIVGVSTAIFIGGPGAVFWMWITAFLG
                     ASSAFIESTLGQIFKRVENNEYRGGPAYYIEYGIGGKFGKIYGIIFAIVTIISVGLLL
                     PGVQSNAIASSMHNAIHVPQWLMGAIVVVILGLIIFGGVRSIANVATAVVPFMAIIYI
                     LMAVIIICINIQEVPALFALIFKSAFGLQSAFGGIVGAMIEIGVKRGLYSNEAGQGTG
                     PHAAAAAEVSHPSKQGLVQAFSVYIDTLFVCTATALIILISGTYNVTDGTVNANGTPH
                     LIKDGGIYVENATGKDYSGTAMYAQAGIDKAFHGSGYQFDPTFSGVGSYFIAFALFFF
                     AFTTILSYYYITETNVAYLTRNQNNQVSSIFINIARVIILFATFYGAVKTADVAWAFG
                     DLGVGLMAWLNIIAIWILHKPAVNALKDYEIQKKRLGNGYNAVYQPDPNKLPNAVFWL
                     KTYPERLKQARAKK"
     gene            1789..>2472
                     /locus_tag="PPY92_002583"
     CDS             1789..>2472
                     /locus_tag="PPY92_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDJ2626524.1"
                     /translation="MSEFKTGKINKHVLYSNILNRDVTISIYLPESYNQLVKYNVILC
                     FDGLDFLRFGRIQRTYESLIKEARIDDAIIVGFHYEDVDKRREEFHPQGSRSHLTIQS
                     VGKEILPFIDSTFSTLKVGNARLLVGDSLAGSIALLTALTYPTIFSRVAMLSPHSDDK
                     VLDKLNQCANKEQLTIWHVIGLDEKDFTLPTNGKRADFLTPNRELAEQIKKYNITYYY
                     DEFEGGHQWK"
BASE COUNT          766 a          344 c          496 g          866 t
ORIGIN      
        1 attctgaaaa atatattgaa gtgtctcgtg cttaatcgta taatattttt atgtttaaac
       61 aataggggga atcttatgat tgaaaaatta gtaacctttt taaatgaggt tgtttggagt
      121 aagccattag tttatggttt gctaattact ggtgtgctat ttacattgcg tatgcgattt
      181 tttcaagtta gacattttaa agaaatgatt cgattaatgt ttcaaggaga gaagtctcct
      241 aatggtattt caagttttca agcgatagcc atgtctttag caggcagggt tggtacaggt
      301 aatattgtcg gtgtatctac tgcaatattt ataggaggac ctggtgcagt attttggatg
      361 tggattactg cgtttttagg tgcaagtagt gcttttattg aatctacact tggtcaaata
      421 ttcaagagag ttgaaaataa tgaataccgt ggtggaccag cgtattatat tgaatatggt
      481 attggtggta aatttggtaa aatttacgga attatctttg ctattgttac gattatctca
      541 gtaggtctat tgcttcctgg tgtgcaatct aacgctatag caagttctat gcataatgcg
      601 attcatgttc cacaatggtt aatgggtgct attgttgtag ttattttggg attaattatt
      661 tttggtggtg tacgtagtat tgccaatgtt gcaacagccg ttgtaccatt tatggcaatt
      721 atttacatac tgatggctgt cattatcatt tgtatcaata tacaagaagt gccagcgtta
      781 tttgcattaa ttttcaaatc agcatttgga ttacaatctg cttttggtgg tatcgttggc
      841 gcaatgatag agattggtgt taaacgtgga ttatactcaa atgaggctgg tcaaggtaca
      901 ggtccacacg cagcagcggc agcagaagta tcacatccaa gtaaacaagg tctagtacaa
      961 gcattttcag tttatattga tacattattt gtatgtactg caactgctct gattatactt
     1021 atttctggta catataatgt gactgatggt acggttaatg cgaatggcac accgcattta
     1081 attaaagatg gcggtattta tgttgaaaat gcaacaggta aagattattc aggtactgcg
     1141 atgtatgcac aagccggcat tgataaagcg ttccatggca gtggttatca atttgatcct
     1201 actttctctg gcgtaggttc gtactttatt gcatttgctt tattcttctt tgcatttact
     1261 acaattttgt cgtactacta cattacagaa acaaatgttg cttatttaac gcgtaatcaa
     1321 aataatcaag tttcatcgat atttattaat attgctcgtg tgattatttt gttcgctaca
     1381 ttttacggtg cagttaaaac agctgatgta gcatgggcat tcggtgattt aggtgtaggt
     1441 ctaatggctt ggttaaatat cattgcgatt tggattttac ataagcctgc cgtaaatgct
     1501 ttaaaagatt atgaaattca aaagaaacgt ttaggaaacg gttataatgc agtttatcaa
     1561 cctgatccga ataaattacc taatgctgtc ttttggttga agacatatcc agaacgttta
     1621 aaacaagcac gtgccaaaaa gtaatctact tttgtttata gtatatgtag tgatcatttg
     1681 ataaaaaaag aaacagcatt gataatttta ggtgctcaga aatttgaatt ttaaaaatat
     1741 agtgtctctt ggtacaataa caatacaact actagggggc acttttttat gtcagaattt
     1801 aaaactggta agattaataa acatgtttta tatagtaata ttttaaatag agatgtcacg
     1861 ataagtattt atttaccaga atcttataat caacttgtta aatataatgt cattctttgc
     1921 tttgacggat tagatttttt acgtttcggg agaatacaac gtacatatga atcgttaatc
     1981 aaagaagcgc gtattgatga tgcgatcatt gttggattcc attatgaaga cgttgataag
     2041 cgtagagagg aatttcatcc acaaggaagt cgttctcatt taactattca atcagtcggt
     2101 aaagaaatat tgccatttat tgactcgacg ttttctacac tgaaagtagg taatgcaagg
     2161 ttattagtag gggatagttt agcgggtagt attgccttat taacggcgtt gacctatcca
     2221 acgattttta gtcgtgtagc aatgttaagt ccacattcag atgacaaagt attagataag
     2281 ctaaatcaat gtgctaataa agaacaattg acaatttggc atgtcattgg tctagatgaa
     2341 aaagatttta ctttaccaac aaatggtaag cgtgccgatt tcttaacacc gaatagagaa
     2401 ttagctgaac aaattaagaa atataatata acttattatt acgatgaatt tgaaggtggt
     2461 caccaatgga aa
//
LOCUS       DANBWH010000269         2461 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.278, whole genome shotgun sequence.
ACCESSION   DANBWH010000269 DANBWH010000000
VERSION     DANBWH010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.278"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(191..1399)
                     /locus_tag="PPY92_002584"
     CDS             complement(191..1399)
                     /locus_tag="PPY92_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDJ2626525.1"
                     /translation="MNENEKNIRKNFLKFTESRYMKFVGGNDLVFSLIALVLLGIVIF
                     IFEKVSYVFDPFIIVFKTIAAPIIVSLILFYLFNPIVNMMERYRIPRVAGISIIYLAV
                     VGVITLIVNLLIPIIGSQVDSLVKNSPQYLEKLINSIDKIANNTFFSSYYSQINDWLN
                     SLPKKIPSMLSEFTDGFGSKIATFAETIANIGVVIVTTPFVLFFMLKDGHHFKEFSTN
                     IMPPKFRKDFHDLLEKMSVQVGSYIQGQIIVSFCIGILLFIGYSVIGLKYSLVLASIA
                     AVTSVVPYLGPTIAISPAIVIAAITSPWMLLKLAVVWTLVQFVEGHFISPNIMGKTLK
                     IHPLTIIFILLCAGKLLGIVGVILGIPGYAILKVLVTHLFQLFKRRYNRFYGNDVGEY
                     DIKESNKIVE"
     gene            complement(1392..1559)
                     /locus_tag="PPY92_002585"
     CDS             complement(1392..1559)
                     /locus_tag="PPY92_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626526.1"
                     /translation="MKKGLINPKLSVLHGRFVTVKGICMVSLMLVILHKWTVFIQHLM
                     NILSGGESIIE"
     gene            complement(1534..2385)
                     /locus_tag="PPY92_002586"
     CDS             complement(1534..2385)
                     /locus_tag="PPY92_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDJ2626527.1"
                     /translation="MGEYIVTKTLNNNVVVCTNNDQEVILIGKGIGFNKKEGMALNDQ
                     TITIEKIYKLESEQQKAHYKSLVEIADDNVLQVIIDSLNFISNTAMNVDSKQLVVSLT
                     DHIIFAYKRLKQNQVISNPFVMETMQLYSDAYHIAKQVIDQLNAALDVHFPEDEIGFI
                     ALHIASNTEDLSMHEMTLINNVIKKGIDIIESDLVTTVDKESLQYQRFIRHVQFLIRR
                     LRRKEYIHAQDDFVSMIKNHYPICYNTAYKILTMIQKQFDVNISESEIIYLTLHIHHF
                     EERINQS"
BASE COUNT          896 a          407 c          310 g          848 t
ORIGIN      
        1 tcgacataat aatgaagtac taatcaagtg gcaaatactc gtttcattat gcatgtgctt
       61 tataaacggc taaactttcg taaaaattta taagctaaac atgtgctgat aatcataaat
      121 gtaactgccc aacattataa gcaccataaa aaataccaac aaacatatag tcattggtat
      181 tttttattac ttattcaact attttattac tttctttaat atcatattca cctacatcat
      241 taccatagaa acgattgtat cgacgtttaa ataattggaa taaatgagta actaatactt
      301 ttaaaatagc atatcccgga atacctaaaa taacgcctac aataccaagc aatttgcctg
      361 cacacagtaa aatgaaaatg attgtaagtg gatgaatctt aagtgtttta cccatgatat
      421 ttggtgaaat gaagtgccct tcaacaaatt gtactaaagt ccatactact gctaatttta
      481 agagcatcca cggcgatgtt atagcagcta ttacaatagc tggagaaatc gctatagtag
      541 gccctaaata tggtacaaca cttgtaactg ccgcaatact agctaatact aagctatatt
      601 tcaacccgat aaccgaataa ccgataaaca acagtatacc gatacagaat gaaacgataa
      661 tttgtccttg aatgtatgaa ccaacttgaa cactcatttt ttcaagtaga tcatgaaaat
      721 cttttcggaa tttcggtggc ataatattcg ttgaaaattc tttgaagtga tgtccatctt
      781 taagcataaa gaatagtaca aatggtgttg tgacaatcac aacgccaata ttagcaatcg
      841 tttctgcaaa cgttgcaatt ttagacccaa agccatctgt aaattcactt aacatagatg
      901 gtattttctt aggtaaagaa tttaaccaat cattaatttg actataatac gaagagaaaa
      961 acgtattatt tgctatttta tcaatagaat taattaattt ttctagatat tgcggtgaat
     1021 ttttaactaa actatctact tgcgaaccaa taataggtat caataaatta acaattaacg
     1081 taataacacc tactacagct agataaataa tagaaatacc tgcaactctt ggtattctat
     1141 aacgttccat catatttacg attgggttaa atagatagaa tagaattaaa gagacgatga
     1201 taggtgctgc tatcgtctta aaaacgatga taaaaggatc aaaaacatat gatacttttt
     1261 cgaaaataaa aataacaata cccaacaata ctagcgctat taatgagaag actaaatcat
     1321 tcccaccaac aaacttcata taccgtgatt cggtaaattt taaaaaattc tttcttatat
     1381 tcttttcatt ttcattcaat tatagattca ccaccactta aaatattcat aagatgttgt
     1441 ataaaaacag tccacttatg taaaattact aacatcaatg ataccataca aatacccttt
     1501 acagtaacga aacgcccgtg taatacactt aatttaggat tgattaatcc tttcttcaaa
     1561 atgatgaatg tgtaatgtta aatatataat ttcagactca ctgatattaa catcaaattg
     1621 tttttgtatc atagttaaaa ttttatatgc tgtgttatag caaatcggat agtgattttt
     1681 aatcatagac acaaaatcat cttgtgcatg tatatattct tttcttctta atcggcgaat
     1741 taaaaattgt acgtgcctta taaaacgttg gtattgtaat gattccttat caacagttgt
     1801 cacaaggtct gattcaatga tatctatacc ttttttaata acattattga tcaaggtcat
     1861 ctcatgcata gataaatctt ctgtattaga tgcaatatgt aatgcaataa atcctatctc
     1921 atcttcagga aaatgtacat ctaatgctgc atttaactga tcaatcacct gtttagcaat
     1981 atgatatgca tcactatata actgcatagt ttccataaca aatggattgc taataacttg
     2041 attttgtttt aagcgtttat aagcaaatat aatatgatcc gttaatgaaa ctacaagttg
     2101 ttttgaatca acattcatcg cagtattaga aataaaattc aacgaatcaa taattacttg
     2161 taatacatta tcatcagcga tttcaactaa acttttataa tgtgcttttt gttgctcact
     2221 ttctaattta taaattttct ctattgtaat agtttggtcg tttaacgcca ttccctcttt
     2281 tttgttaaaa ccaatacctt taccgattaa aataacttct tgatcattat tagtacatac
     2341 tacgacattg ttgttcaatg ttttagtaac aatatattct cccattatca tcacctattt
     2401 ttctattatt aagattatat atcggaaatg tctaacttga aagtataata atttaaatac
     2461 t
//
LOCUS       DANBWH010000270         2434 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.279, whole genome shotgun sequence.
ACCESSION   DANBWH010000270 DANBWH010000000
VERSION     DANBWH010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.279"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..546)
                     /gene="qacR"
                     /locus_tag="PPY92_002587"
     CDS             complement(<1..546)
                     /gene="qacR"
                     /locus_tag="PPY92_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001807342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug-binding transcriptional regulator
                     QacR"
                     /protein_id="HDJ2626528.1"
                     /translation="MNLKDKILGVAKELFIKNGYNATTTGEIVKLSESSKGNLYYHFK
                     TKENLFLEILNIEESKWQEQWKKEQIKCKTNREKFYLYNELSLTTEYYYPLQNAIIEF
                     CTEYYKTNSINEKMNKLENKYIDAYHVIFKEGNLNGEWCINDVNAVSKIAANAVNGIV
                     TFTHEQNINERIKLMNKFSQIF"
     gene            724..2268
                     /locus_tag="PPY92_002588"
     CDS             724..2268
                     /locus_tag="PPY92_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux MFS
                     transporter QacB"
                     /protein_id="HDJ2626529.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPE
                     LVRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIF
                     FAESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIVSSIGAVFG
                     PIIGGALLEQFSWHSAFLINVPFVIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIA
                     GMIGLVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSF
                     SAGTIAAFMTMFAMTSVLLLASQWLQVVEELSPFKAGLYLLPMEIGAMVFAPIAPGLA
                     ARFGPKIVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGTASLAVASALIML
                     ETPTSKAGNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHV
                     AEESVVGAVEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSL
                     DITKQK"
BASE COUNT          751 a          356 c          412 g          915 t
ORIGIN      
        1 aaaaatttga gagaacttgt tcataagttt aattctttca ttaatatttt gttcatgtgt
       61 aaaggtaaca attccattaa cagcgtttgc tgctatttta ctaacagcat taacatcatt
      121 aatacaccat tcgccattta aattaccctc cttaaaaatt acatgataag catctatata
      181 tttgttttct aatttattca ttttttcatt aatgctatta gttttataat attcagtgca
      241 aaattcaatt attgcgtttt gaagtggata atagtattcg gttgttaaag atagttcatt
      301 atataaataa aatttttctc tattagtttt acatttgatt tgttcctttt tccactgttc
      361 ttgccattta gattcttcta tatttaaaat ttctaaaaat agattttctt ttgttttaaa
      421 gtgataataa agattccctt tactactttc tgataattta acaatttctc cagtagtagt
      481 ggcattatat ccatttttta taaataattc ctttgcgaca cctagtattt tatctttcaa
      541 gttcatcacg cctttaatga gtattaagta ctatcaatga tagcacaaaa aattcaaaga
      601 ataattgtat aacacaatac agccatttaa atttcgcaag attttttgtt gtaatatgta
      661 aaaaaataga ttataatcct tatagaccga tcgcacggtc tataaggatt ggagggaact
      721 taaatgattt cattttttac aaaaactact gatatgatga catcaaaaaa aagatggact
      781 gcactagtag tattagctgt tagtttgttt gttgttacaa tggatatgac aatattaatt
      841 atggctttac cggaattagt aagagagtta gagccttctg gtacccaaca gttatggata
      901 gttgatatat actctcttgt tttagctggc tttataattc cattgagtgc ctttgctgat
      961 aaatggggaa gaaaaaaagc attattaact ggatttgctt tatttggcct cgtttcatta
     1021 gctatatttt tcgcagaaag tgcagagttc gtaatagcta ttcgattttt acttggtatt
     1081 gcaggtgctt taataatgcc aactactctt tcaatgataa gagtaatttt tgaaaaccct
     1141 aaagaaaggg ccactgcatt agctgtatgg tcaatcgttt catcgatagg tgctgttttt
     1201 ggaccaatta tcggaggagc tttacttgag caattttcat ggcactcggc atttttaatt
     1261 aatgtaccgt ttgtgataat agcagttgta gcaggtttat ttttattacc agagtctaag
     1321 ttatcaaaag aaaagtctca ctcgtgggat attccttcta caattttatc aattgcaggc
     1381 atgattggac tggtatggag tatcaaagaa ttttcaaaag aaggactagc agatattatt
     1441 ccatgggttg taatagtatt agcaattacc atgatagtga tatttgttaa acgtaattta
     1501 tcaagttctg atccaatgtt agacgtaaga ctttttaaaa agagatcatt ttcagctggt
     1561 acaattgctg catttatgac aatgtttgca atgacatctg ttttgttatt agcttcacaa
     1621 tggttacagg ttgtggaaga actttctcct tttaaagctg gcttatacct attacctatg
     1681 gaaataggag ctatggtgtt tgcaccaatt gcacccggat tagcggcgcg atttggaccg
     1741 aaaatagtgt taccttccgg aattggaatt gcagccattg gcatgtttat tatgtatttc
     1801 tttggtcatc cattatcata ttctacaatg gctttagcat taattttagt tggagctggt
     1861 acggcttcac tagcagttgc atctgctcta ataatgttag aaacacctac atcaaaagca
     1921 ggtaatgcag ctgctgttga agagtctatg tatgaccttg gaaatgtttt tggtgtagca
     1981 gtacttggta gcctatcttc tatgctttat cgtgtatttt tagatatttc atctttttca
     2041 tcaaaaggta tagttggaga tttagctcat gtagctgaag aatctgtagt gggcgctgtc
     2101 gaagtagcta aagctacggg gataaaacag cttgcaaacg aggctgtaac atcatttaat
     2161 gatgcttttg tagcaactgc tttagtaggt gggattatca tgattatcat ttcaatagtt
     2221 gtctatttgt taattcccaa atcacttgat ataactaaac aaaaatagta ctaaacatat
     2281 atttttaggt tctgttgcaa agttaaaatt atatgcacat ctatacaaaa ctataaaagc
     2341 aaatattgtt ttaacagtaa agagttttgt tagtattaat tatttttcgt ttttcagtat
     2401 aaatattaca tgatttttaa ctttgcaaca gaac
//
LOCUS       DANBWH010000271         2434 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.280, whole genome shotgun sequence.
ACCESSION   DANBWH010000271 DANBWH010000000
VERSION     DANBWH010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.280"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            111..2390
                     /gene="secDF"
                     /locus_tag="PPY92_002589"
     CDS             111..2390
                     /gene="secDF"
                     /locus_tag="PPY92_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HDJ2626530.1"
                     /translation="MKKSSRIIAFLLLVVLLFAGMAATYKSVIKNVNLGLDLQGGFEV
                     LYQVDPLNKGDKIDKKALQSTAQTLENRVNVLGVSEPKIQVEEPNRIRVQLAGVTDQN
                     EARKILSSQANLTIRDAEDKVKLSGSDIKQGSAKQEFKQETNQPTVTFKVKDKNKFKK
                     VTEEISKKRDNVMVVWLDFKKGDSYKKEAQKKNPKFISAASVDQPINSDSVEISGGFK
                     GQEGVKKAKQIAELLNAGSLPVDLKEIYSNSVGAQFGQDALDKTVFASFIGVALIYLF
                     MLGFYRLPGLVAIIALTTYIYLTLVAFNFISGVLTLPGLAALVLGVGMAVDANIIMYE
                     RIKDELRIGRTIKQAFSKANKSSFLTIFDSNLTTVIAAAVLFFFGESSVKGFATMLLL
                     GILMIFVTAVFLSRFLLSLLVSSNIFKNQFWLFGVKKNKRHDINEGVDVHDLKTSFEK
                     WNFVKLAKPLIGVSILIVVVGLVILYIFKLNLGIDFSSGTRVDFQSKQAITQQKVEQV
                     VKDSGLKADQIQINGKDNKVATVQFKDDLTRAQDNKLSDNIKSKFGDTPQINTVSPII
                     GQELAKNAMLALIYTSIGIIIYVSLRFEWRMGLSSVLALLHDVFIIVAIFSLFRIEVD
                     LTFIAAVLTIVGYSINDTIVTFDRVRENLQKVKVITTTEQIDDIVNRSIRQTMTRSIN
                     TVLTVIVVVVAILFFGAPTIFNFTLALFIGLISGVFSSIFIAVPLWGIMKKRQLKKSP
                     KHKLVVYKEKKSNDEKILV"
BASE COUNT          866 a          340 c          439 g          789 t
ORIGIN      
        1 tagtatgtta atatgtataa ggctaataaa ttttgtaaga aagatttaac ttgcgttata
       61 aaatagtatt caagtattct aaatacatac gtaaataatg aggtgttcat gtgaagaaaa
      121 gtagtagaat aattgcgttc ttgcttttag tcgtattgtt gtttgccgga atggctgcaa
      181 cttataaaag tgttataaag aatgtcaatt taggacttga tttgcaaggt ggatttgagg
      241 tgctttatca agtcgatcct ttaaataaag gcgacaaaat agataaaaaa gccttacaat
      301 caacagcgca aacattagaa aatcgtgtta acgttttagg tgtttcggaa cctaaaattc
      361 aagttgaaga acctaataga attagagttc aacttgctgg tgtaacagac cagaatgaag
      421 cacgtaaaat tttatcttct caagctaatt taacaattcg cgatgcagaa gataaagtaa
      481 aattaagcgg ttctgatatt aaacagggat ctgccaaaca agaatttaaa caggaaacaa
      541 accagcctac agttactttt aaagtaaaag acaaaaacaa atttaaaaaa gtaacggaag
      601 aaatctctaa gaaacgtgat aatgtcatgg ttgtttggtt agatttcaaa aaaggtgaca
      661 gctataaaaa agaagcacaa aagaaaaacc ctaaatttat ttctgcagca agtgttgatc
      721 aaccaatcaa ttctgatagc gtagaaatct cgggtggatt taaaggacaa gaaggcgtta
      781 aaaaagcaaa acaaatcgcc gaattgttaa atgcaggttc tttaccagtt gacttgaaag
      841 aaatttattc aaactcagta ggtgcccaat ttggtcaaga tgcattagat aaaactgtgt
      901 ttgcatcgtt tattggtgta gcattaattt atttattcat gcttggattc taccgattac
      961 ctggtttagt agcgattatt gcattgacaa cttatatcta tctaacgtta gtagcattta
     1021 atttcatttc cggggttcta actttaccag gattagcggc gttggtatta ggtgtaggta
     1081 tggctgtaga tgccaatatt atcatgtatg agcgtattaa ggatgaactt cgaataggta
     1141 gaacgataaa gcaagccttt tctaaagcaa acaaaagttc attcctaaca atttttgatt
     1201 ctaacttaac aacagttatc gccgcagcag tattattctt cttcggtgaa agttcagtta
     1261 aaggtttcgc gacaatgtta ttattaggta ttctaatgat ctttgttaca gccgtgttct
     1321 tatcaagatt cttattatca ttacttgttt catcaaatat attcaaaaat caattttggt
     1381 tatttggtgt taaaaagaat aaacgacatg atattaatga aggtgtagat gttcatgacc
     1441 ttaaaacttc attcgagaaa tggaattttg ttaaattagc taagccatta attggagtaa
     1501 gtattttaat tgtagttgtc ggtttagtta ttctttatat cttcaagtta aacttaggaa
     1561 ttgatttctc aagtggtaca cgtgtagatt tccaatctaa acaagcaatt actcaacaaa
     1621 aggttgagca agttgtaaaa gatagtggac ttaaagcgga tcaaattcaa atcaatggta
     1681 aagataataa agtagcaact gttcaattta aagacgattt aacgcgtgct caagataata
     1741 aattgagtga taatattaaa tcgaaattcg gtgacacacc tcaaattaat actgtatcac
     1801 caattattgg acaggaatta gcaaaaaatg caatgcttgc attaatctat acatcgatag
     1861 gtatcatcat ctatgtatca ttacgatttg aatggcgcat gggtctttca tctgtattgg
     1921 cattattaca tgatgtattc attatagtag cgattttcag tttatttaga attgaagtag
     1981 atttaacatt tatcgccgct gtattaacaa ttgtcggtta ttcaattaat gatacaatcg
     2041 taacgtttga ccgtgtacgt gaaaacttac aaaaggttaa agtgattacg acaacagaac
     2101 aaattgatga tatcgttaat agatcaatta gacagacaat gacacgttca attaatacag
     2161 tattaacagt tattgtagta gtagttgcta tactattctt cggtgctcct acgatattca
     2221 actttacttt agcattattt atcggattga tttctggtgt attctcttca atcttcattg
     2281 ccgttccgct atggggaata atgaaaaaac gtcagttgaa aaaatcgccg aaacacaaat
     2341 tagttgtata taaagaaaag aaatcgaacg atgaaaagat tttagtttaa aatgaattaa
     2401 gcggtatgtg aaacaataaa gagttcacat accg
//
LOCUS       DANBWH010000272         2409 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.281, whole genome shotgun sequence.
ACCESSION   DANBWH010000272 DANBWH010000000
VERSION     DANBWH010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.281"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..639
                     /locus_tag="PPY92_002590"
     CDS             <1..639
                     /locus_tag="PPY92_002590"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HDJ2626531.1"
                     /translation="SFEQHKVWRKRVMKDMGVRKGTKALDVCCGTGDWTIALSKAVGP
                     TGEVTGIDFSENMLEVGKEKTASMENVKLVHGDAMELPFEDNSFDYVTIGFGLRNVPD
                     YLVALKEMNRVLKPGGMVVCLETSQPTLPVFKQMYALYFKFVMPIFGKLFAKSKEEYE
                     WLQQSTFNFPGKEELKRMFEEAGFINVRVRSFTGGVAAMHLGYKEKDNTKGD"
     gene            641..1600
                     /locus_tag="PPY92_002591"
     CDS             641..1600
                     /locus_tag="PPY92_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDJ2626532.1"
                     /translation="MAKLNMNNEIKKVEQRLEKAIKSKDSVLEQASLHLLSSGGKRVR
                     PAFVILSSQFGKDEQTSEQTYQVAVALELIHMATLVHDDVIDKSDKRRGKLTISKKWD
                     QTTAILTGNFLLALGLEHLMAVKDNRVHQLISESIVDVCRGELFQFQDQFNSQQTIIN
                     YLRRINRKTALLIQISTEVGAITSQSDKETVRKLKMIGHYIGMSFQIIDDVLDFTSTE
                     KKLGKPVGSDLLNGHITLPILLEMRKNPDFKLKIEQLRRDSERKEFEECIQIIRKSDS
                     IDEAKAVSSKYLSKALNLISELPDGHPKSLLLSLTKKMGSRNT"
     gene            1692..2141
                     /gene="ndk"
                     /locus_tag="PPY92_002592"
     CDS             1692..2141
                     /gene="ndk"
                     /locus_tag="PPY92_002592"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDJ2626533.1"
                     /translation="MERTFLMIKPDAVQRNLIGEVISRIERKGLKLVGGKLMQVPMEL
                     AETHYGEHQGKPFYNDLISFITSAPVFAMVVEGEDAVNVSRHIIGSTNPSEASPGSIR
                     GDLGLTVGRNIIHGSDSLESAEREINLWFNENEITSYASPRDAWLYE"
BASE COUNT          871 a          320 c          489 g          729 t
ORIGIN      
        1 agttttgagc agcataaagt atggagaaaa cgcgtcatga aagacatggg tgttagaaaa
       61 gggacgaaag cattagatgt ttgttgtggt actggtgatt ggacaatcgc attaagcaaa
      121 gctgtaggac caacaggtga agttactggt attgacttta gtgagaatat gttagaagta
      181 ggaaaagaaa aaactgcttc aatggaaaat gttaaacttg ttcatggtga tgcaatggaa
      241 ttgccgtttg aagacaattc ttttgattat gtaacaattg ggtttggatt aagaaatgtg
      301 ccagactatt tagtcgcgtt aaaagaaatg aatagagtac ttaaaccagg tggtatggtg
      361 gtatgtcttg aaacgagcca accaactttg ccagtcttta aacaaatgta tgcactatac
      421 tttaaatttg ttatgcctat ttttggaaaa ttatttgcaa aatcaaaaga agaatatgaa
      481 tggttacagc aatctacgtt taattttcct ggaaaagaag agttgaagcg catgtttgaa
      541 gaagcaggtt tcataaatgt aagagtacgt agttttacag ggggcgttgc tgcaatgcac
      601 cttggctata aagaaaaaga taataccaaa ggtgattaac gtggcaaagt taaacatgaa
      661 caatgaaatt aagaaagtgg aacaacgact tgaaaaggca ataaaaagta aagattctgt
      721 attagaacag gcatcattac acttattgtc ttctggtggt aaaagagtac gcccagcatt
      781 tgttattctg agtagccaat ttggcaaaga tgaacaaacg tctgaacaaa cgtatcaagt
      841 tgcagtcgca ttagagttaa ttcatatggc aacacttgtt catgatgacg ttattgataa
      901 aagcgacaag cgtcgaggca agttaaccat atcaaagaaa tgggatcaga caactgctat
      961 tttaactggg aattttttat tggcattagg acttgaacac ttaatggccg ttaaagataa
     1021 tcgtgtacat caattgatat ctgaatctat cgttgatgtt tgtagagggg aacttttcca
     1081 atttcaagac caatttaaca gtcaacagac aattattaat tatttacgac gtatcaatcg
     1141 caaaacagca ctgttaattc aaatatcaac tgaagttggt gcaattactt ctcaatctga
     1201 taaagagact gtacgaaaat tgaaaatgat tggtcattat ataggtatga gcttccaaat
     1261 cattgatgat gtattagact tcacaagtac cgaaaagaaa ttaggtaagc cggtcggaag
     1321 tgatttgctt aatggtcata ttacgttacc gattttatta gaaatgcgta aaaatccaga
     1381 cttcaaattg aaaatcgaac agttacgtcg tgatagtgaa cgcaaagaat ttgaagaatg
     1441 tatccaaatc attagaaaat ctgacagcat cgatgaggct aaggcagtaa gttcgaagta
     1501 tttaagtaaa gctttgaatt tgatttctga gttaccagat ggacatccga aatcactact
     1561 tttaagtttg acgaaaaaaa tggggtcaag aaacacatag tatttatgaa aagtattgaa
     1621 agcgctttac caacctgtta atatataata gtaatataca taaacctatt aaacacaggg
     1681 ggatacataa agtggaacgt acatttttaa tgattaaacc agacgcagta caaagaaatc
     1741 taattggtga agtaatttca agaattgaaa gaaaaggact aaaacttgtc ggtggtaaat
     1801 taatgcaagt accaatggaa cttgctgaaa cacattatgg tgaacaccaa ggcaaaccgt
     1861 tttataatga tttaatttca tttattacat cagcaccagt gttcgcaatg gtagttgaag
     1921 gtgaagatgc agttaatgta tctagacata ttattggcag caccaatcct tcagaagctt
     1981 caccaggatc aattagaggt gatttaggtt taactgttgg tagaaatatc attcacggtt
     2041 cagattcatt agagtctgct gaacgtgaaa ttaatctatg gtttaatgaa aatgaaatta
     2101 ctagctatgc ttcaccacgt gatgcatggt tatatgaata aaatataaac tgtaaacctt
     2161 tacgatttat ttataaaggt agaaagggtt ttgttatgtg gttagtcatt atgattatac
     2221 ataacaaggc ccgtttttta tgttgtagta aattacttga aaaattttat agttttttgg
     2281 taacacgtat taaaaagaga ggaatattct ttatcaaatg aaactaaaca gagagaaggg
     2341 gttgttaaaa tgaagaatat tatttcgatt attttgggga ttttaatgtt cttaaaatta
     2401 atggaatta
//
LOCUS       DANBWH010000273         2405 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.282, whole genome shotgun sequence.
ACCESSION   DANBWH010000273 DANBWH010000000
VERSION     DANBWH010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.282"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..849
                     /locus_tag="PPY92_002593"
     CDS             <1..849
                     /locus_tag="PPY92_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDJ2626534.1"
                     /translation="HPMLSLFFFYSAFAVPFTFKNNVKKTATNLFILTMIICTIITYL
                     LYNNYFVAMMVYYVVISLIMLDNFKKMKNREYQKEIAEKNRHINTLIAEQERHRIGQD
                     LHDTLGHVFASLSLKSELAYKLIDTDVEKVKAELLAINKLSRESLNKVREIIDDVKLP
                     SFIEEIDSIRKVLKDADIDFTFENKELAQVLSPTKQSMLVMITREAINNVIKHANASK
                     VHGKLKTVNNHKLLLMIEDDGKGIDSDCEVKSISQRVQHLNGTLAVDSTNGTKIIIEI
                     STGGIA"
     gene            846..1448
                     /locus_tag="PPY92_002594"
     CDS             846..1448
                     /locus_tag="PPY92_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDJ2626535.1"
                     /translation="MTSLIIAEDQNMLRQAMVQLIKLHGDFEILADTDNGLDAMKLIE
                     EYNPNVVILDIEMPGMTGLEVLAEIRKKHLNIKVIIVTTFKRPGYFEKAVVNDVDAYV
                     LKERSIEELVETINKVNNGEKEYSATLMTSFFVDKNPLTPKEQIVLREIGNGLSSKEI
                     SEKLFLTDGTVRNYTSVIIDKLFADNRFDAWKKANEKGWI"
     gene            complement(1561..1749)
                     /locus_tag="PPY92_002595"
     CDS             complement(1561..1749)
                     /locus_tag="PPY92_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HDJ2626536.1"
                     /translation="MNISNENTVTLIAVIIAIIIGIFLQIFFKLPLIVTAVLSILLGI
                     FVGFIVYLIVSFYNKRKN"
     gene            1888..>2405
                     /locus_tag="PPY92_002596"
     CDS             1888..>2405
                     /locus_tag="PPY92_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDJ2626537.1"
                     /translation="MKSNKSLAMIVVAIIIVGVLAFQFMNHTGPFKKGTNHETVQDLN
                     GKDKVHVQRVVDGDTFIANQNGKEIKVRLIGVDTPETVKPNTPVQPFGKEASNYSKKT
                     LTNQDVYLEYDKEKQDRYGRTLAYVWISKDRMYNKELVEKGLAREKYFSPNGKYRNVF
                     IEAQNKAKQQKL"
BASE COUNT          955 a          278 c          420 g          752 t
ORIGIN      
        1 catccaatgc taagtttgtt tttcttttat agtgcttttg ccgttccatt tacttttaaa
       61 aataatgtta aaaaaacggc aactaatctt ttcatactaa caatgattat atgtacaata
      121 ataacgtact tattgtataa caactatttt gttgcaatga tggtttatta tgtcgttata
      181 tcgttaataa tgctagataa ttttaaaaaa atgaaaaacc gtgaatatca aaaagaaata
      241 gcagaaaaaa atagacatat taatacatta attgctgaac aagagcgaca tagaattggt
      301 caagacttac atgatacgtt agggcatgtg tttgcaagtt tatcattaaa atcagaatta
      361 gcttataaac taatagatac tgatgtagaa aaagtaaaag ctgaattatt agcaattaat
      421 aaattatctc gtgaatcatt gaacaaagtt cgagaaatta ttgatgatgt aaaattacca
      481 tcatttattg aagagattga tagtatacgt aaagttttaa aagatgctga tattgatttt
      541 acatttgaaa ataaagaatt agcgcaagta ttaagtccta ctaaacaatc tatgttagtt
      601 atgattacgc gtgaagcgat aaataatgtt attaaacatg caaatgcttc aaaagttcat
      661 ggtaaattaa aaactgtaaa caatcataaa ttactgctta tgattgaaga tgatggcaaa
      721 ggtatcgata gtgattgtga ggtgaaaagt atttcacagc gtgtacaaca tttaaatgga
      781 actttagcag tcgactcaac aaatggaact aaaataatca ttgaaatctc aacaggagga
      841 atagcatgac atctttaatt attgcagaag atcaaaatat gttacgacag gcaatggttc
      901 aattaattaa actacatggt gattttgaaa ttttagcaga tactgataat ggtctcgatg
      961 caatgaaact tattgaagaa tataatccta acgttgttat tttagatata gaaatgccag
     1021 gcatgactgg acttgaagtt ttagcggaaa ttagaaaaaa gcatttgaat attaaagtga
     1081 ttattgtaac aacttttaaa agaccgggat actttgaaaa agcagttgtg aatgatgtgg
     1141 atgcatatgt tttaaaagaa cgttctatag aagaattggt ggaaaccatt aataaagtaa
     1201 ataacggaga gaaagaatat agcgccacat tgatgacttc attttttgta gataaaaacc
     1261 cattaacgcc caaagaacaa attgtattaa gggaaattgg caatggttta agtagtaaag
     1321 aaataagtga aaaattattt ttgacagatg gaacagttag aaattataca tctgttataa
     1381 ttgataaatt atttgcagat aatcgttttg atgcttggaa aaaggcaaat gaaaaaggct
     1441 ggatctaaat acaaacaaaa aagtattgag tgttttgatt taagagactt ggaaagtgag
     1501 tcaataatca aaatcttact caatactttt attttggtac tcgcaattta gatttgttaa
     1561 ctaatttttt cgcttattat aaaacgaaac aatcaagtaa acgataaagc ctacaaagat
     1621 acccaataaa atagatagta ctgccgtcac tattaatggt aatttgaaaa atatttgtag
     1681 gaaaatacca atgataattg cgataattac tgcaattaat gtgactgtat tttcatttga
     1741 aatgttcatt tattttcact ccttaacaat aacattatat catgctatag ctttccaaaa
     1801 tattgaaata tgtagatatg gctattgacg atatttctta acttttatat gattaatcgg
     1861 aatgaaaaaa gagaagtagg tggcaatatg aagtcaaata aatcgcttgc tatgattgtg
     1921 gtagccatca ttattgtagg tgtattagca tttcaattta tgaatcatac gggtcctttc
     1981 aaaaagggga cgaatcatga aactgtacaa gatttaaatg gtaaagataa agtacatgtt
     2041 caaagagttg tggatggtga tacatttatt gcaaatcaaa atggtaaaga aattaaagtt
     2101 aggcttatag gggttgatac gccagaaacg gtgaaaccga atacgcctgt acaaccattt
     2161 ggcaaagaag catcaaatta tagtaagaag acattaacaa atcaagatgt ttatttagaa
     2221 tatgataaag aaaaacaaga tcgctatggt agaacattgg cgtatgtatg gataagtaaa
     2281 gatcgtatgt acaataagga attagtggaa aagggacttg ctagagagaa gtatttttca
     2341 ccaaatggca aatatagaaa tgtatttata gaagcacaaa ataaagctaa acaacagaaa
     2401 ttaaa
//
LOCUS       DANBWH010000274         2382 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.283, whole genome shotgun sequence.
ACCESSION   DANBWH010000274 DANBWH010000000
VERSION     DANBWH010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.283"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            177..671
                     /locus_tag="PPY92_002597"
                     /pseudo
     CDS             177..671
                     /locus_tag="PPY92_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777469.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            662..823
                     /locus_tag="PPY92_002598"
                     /pseudo
     CDS             662..823
                     /locus_tag="PPY92_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066885.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(801..902)
                     /locus_tag="PPY92_002599"
     CDS             complement(801..902)
                     /locus_tag="PPY92_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626538.1"
                     /translation="MQGFKEKHQELKKALCQIGLMSSISEVRQLNIA"
     gene            complement(1025..1120)
                     /gene="pepA1"
                     /locus_tag="PPY92_002600"
     CDS             complement(1025..1120)
                     /gene="pepA1"
                     /locus_tag="PPY92_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin
                     PepA1"
                     /protein_id="HDJ2626539.1"
                     /translation="MMLIFVHIIAPVISGCAIAFFSYWLSRRNTK"
     gene            1265..2277
                     /locus_tag="PPY92_002601"
                     /pseudo
     CDS             1265..2277
                     /locus_tag="PPY92_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611271.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          960 a          347 c          377 g          698 t
ORIGIN      
        1 atataatcta acatcattaa ctctctcaaa ttagacaaaa caaaaccaaa atactgagtc
       61 attcaatcat caagacagtt taactggaaa tgataattta ataatgtgca cttttgattg
      121 tttaaacatg tacaattttt aaaaacctat agataatttt attgagatga acaacaatga
      181 aattatcttt agacataaat actgattttg aagttactac tcttacagat ttaccaaaat
      241 taaaaattgt catggagaac ttaaacatga aaataaacaa aagtgaaata gcgagacaca
      301 tgggtgttta tagaagaaca gttgataaat atttaaatgg ctttgagcca accaaaaaga
      361 gaaatcgtca gtctattatt gacaagtatt atcctatcat cgaaaaatta ctttcagact
      421 ctagtgaaca aaagttctac tataagctta ttctttggca atatttgaaa gataaacatg
      481 gattaacctg tgcatattct acatttagag cttatatcct taaacatgat gaatttaatc
      541 gttattttat gaaaggttat caacggttgt cgcctaaagg caaaacgaga ttcgagacaa
      601 aagccagtca tcaagcacag tttgactgga aagaaggcat taactttaaa acgaaggata
      661 atcaaatggt cctttagaag ggataaacaa caaaataaaa ttaattaaac gtacatcttt
      721 tggttaagga agttataatc atttgcgaaa tcgaatatta ttatgttcaa aactttacgc
      781 tccaaaaagt aaaaaggaag ctaagcaatg tttagttgcc taacttccga tattgaactc
      841 atcaggccaa tttggcatag agcctttttt agttcttgat gtttctcttt aaaaccttgc
      901 atattttaca aagagaaaga ttagcagtat aattgagata acgaaaataa gtatttactt
      961 atacaccaat cccctcacta tttgcggtag tgaggggatt tttattggtg cggctatatg
     1021 tcacctattt tgtattgcgt ctacttagcc aataagaaaa aaacgcaatg gcacagccac
     1081 tgatgactgg tgctatgatg tgaacgaaaa taagcatcac cttatacacc tcctctctgc
     1141 gtctaaattg acgcctgaga gataggcgac tctactatta tatcatctaa ttggacaaat
     1201 tctatgagag tagattttgt taatttaaga aagaagaaca tttagttcag agtttaagtt
     1261 ataaatggtt agattatatg aaaatggtaa gccaagaaat gaaattatac gcgagtatga
     1321 tttaacacct ttgacgttag gaaaatggat aaagtaacat caaaacaagg gtacattcaa
     1381 tcaccaagat aacttaacag atgaagaaaa agagctgatt atggggcaaa aatagaagtc
     1441 attcaaaaga atgcacatca atattcagta ccagcaatgt gtaaagtcct aaaaatacca
     1501 agaagtacct attatgattc tataaaaaga aaagataata aaatcactaa agatgattca
     1561 aacgtagaac gtgccgccat aaatattttt aattctaata gaaaagtctt tagtacaaga
     1621 cgaattaaaa atcatttaaa tgacaagggt ctcactgtat ctggacaaaa gataggtcga
     1681 ttatgaaaaa atctagtttc tgtttatatg aaagctaaat acaaaaatca tcaaatagaa
     1741 actaatgaaa aacgaattaa aaatcatttg aatcgcgctt ttaatagaga acaaccaatg
     1801 gagacattag taagtgattt gacatatgta aaagtcggag gaacatggca ttacatatgt
     1861 ttatttatag atctatttaa tagagaaatt gttggttaca gtgcaggtaa aaataaggac
     1921 gccaatttag tatcaaaagc aatcagcaga ataaatcata atcttgaaca aatcaaacta
     1981 ttccacactg atagagacaa agaatttgat aatcatttga tagatgaagt cctagaaaca
     2041 tttaaaataa aacgttcatt aagtaccaaa ggttgtcctt atgataacgc agttgcggaa
     2101 gcaacgatga aagcaatgaa aacagaattt gtaaaacaaa tgcaatttga aaacttagaa
     2161 cagttagaaa cagaattatt tgattatgta aattggtaca acaattttag accacattct
     2221 tcattacagt atttgacgcc agtggcattt aaaaatatac acatgaaaac tgtctaaaaa
     2281 atgttgacat accactaagt gaactattaa aaccaaaaga aatttaatac aattcaagac
     2341 catgcaaact catttataaa cattgtccgt ttatagatag gt
//
LOCUS       DANBWH010000275         2341 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.284, whole genome shotgun sequence.
ACCESSION   DANBWH010000275 DANBWH010000000
VERSION     DANBWH010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.284"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..374
                     /locus_tag="PPY92_002602"
     CDS             <1..374
                     /locus_tag="PPY92_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDJ2626540.1"
                     /translation="KKNSKSFREAVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKAC
                     ASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGA
                     VLMDLHDEWIYSSRKYINFDK"
     gene            431..823
                     /locus_tag="PPY92_002603"
     CDS             431..823
                     /locus_tag="PPY92_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDJ2626541.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            824..2263
                     /locus_tag="PPY92_002604"
                     /note="aph(2'')-Ia"
     CDS             824..2263
                     /locus_tag="PPY92_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HDJ2626542.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          965 a          213 c          399 g          764 t
ORIGIN      
        1 ctaaaaaaaa ttcaaaatct ttcagagaag ctgttaaagg aatttttaag ttcacagata
       61 ttaacttagc gcgtgaggct aaaaatcgat tgattcatga ttatatcgat caaccaaaat
      121 attcaaaagc ttgcgcatca ttggatgatg gattcgaaga cgcctttcaa tataccgtac
      181 aaggaaattc ccacaatcga ctaaagagta ccaatctaat tgaacgactg aatcaagaag
      241 tacgcagaag agaaaagatt attcgcatct tccccaatca aacatcagcc aatcgcttaa
      301 ttggagccgt tcttatggac ctacatgatg aatggattta ttcttcaaga aaatacatca
      361 attttgataa gtagaaatgg taaaaacatt gtatagcatt ttacacagga gtctggactt
      421 gactgagttt atggaagaag ttttaattga tgataatatg gtttttgata ttgataattt
      481 aaaaggattt cttaatgata ccagttcatt tgggtttata gctaaagaaa ataataaaat
      541 tataggattt gcatattgct atacactttt aagacctgat ggaaaaacaa tgttttattt
      601 acactcaata ggaatgttac ctaactatca agacaaaggt tatggttcaa aattattatc
      661 ttttattaag gaatattcta aagagattgg ttgttctgaa atgtttttaa taactgataa
      721 aggtaatcct agagcttgcc atgtatatga aaaattaggt ggtaaaaatg attataaaga
      781 tgaaatagta tatgtatatg attatgaaaa aggtgataaa taaatgaata tagttgaaaa
      841 tgaaatatgt ataagaactt taatagatga tgattttcct ttgatgttaa aatggttaac
      901 tgatgaaaga gtattagaat tttatggtgg tagagataaa aaatatacat tagaatcatt
      961 aaaaaaacat tatacagagc cttgggaaga tgaagttttt agagtaatta ttgaatataa
     1021 caatgttcct attggatatg gacaaatata taaaatgtat gatgagttat atactgatta
     1081 tcattatcca aaaactgatg agatagtcta tggtatggat caatttatag gagagccaaa
     1141 ttattggagt aaaggaattg gtacaagata tattaaattg atttttgaat ttttgaaaaa
     1201 agaaagaaat gctaatgcag ttattttaga ccctcataaa aataatccaa gagcaataag
     1261 ggcataccaa aaatctggtt ttagaattat tgaagatttg ccagaacatg aattacacga
     1321 gggcaaaaaa gaagattgtt atttaatgga atatagatat gatgataatg ccacaaatgt
     1381 taaggcaatg aaatatttaa ttgagcatta ctttgataat ttcaaagtag atagtattga
     1441 aataatcggt agtggttatg atagtgtggc atatttagtt aataatgaat acatttttaa
     1501 aacaaaattt agtactaata agaaaaaagg ttatgcaaaa gaaaaagcaa tatataattt
     1561 tttaaataca aatttagaaa ctaatgtaaa aattcctaat attgaatatt cgtatattag
     1621 tgatgaatta tctatactag gttataaaga aattaaagga acttttttaa caccagaaat
     1681 ttattctact atgtcagaag aagaacaaaa tttgttaaaa cgagatattg ccagtttttt
     1741 aagacaaatg cacggtttag attatacaga tattagtgaa tgtactattg ataataaaca
     1801 aaatgtatta gaagagtata tattgttgcg tgaaactatt tataatgatt taactgatat
     1861 agaaaaagat tatatagaaa gttttatgga aagactaaat gcaacaacag tttttgaggg
     1921 taaaaagtgt ttatgccata atgattttag ttgtaatcat ctattgttag atggcaataa
     1981 tagattaact ggaataattg attttggaga ttctggaatt atagatgaat attgtgattt
     2041 tatatactta cttgaagata gtgaagaaga aataggaaca aattttggag aagatatatt
     2101 aagaatgtat ggaaatatag atattgagaa agcaaaagaa tatcaagata tagttgaaga
     2161 atattatcct attgaaacta ttgtttatgg aattaaaaat attaaacagg aatttatcga
     2221 aaatggtaga aaagaaattt ataaaaggac ttataaagat tgattatata atatatgaaa
     2281 agctattata aaagacatta gtattaaata gtttaaaaaa atgaaaaata ataaaggaag
     2341 t
//
LOCUS       DANBWH010000276         2339 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.285, whole genome shotgun sequence.
ACCESSION   DANBWH010000276 DANBWH010000000
VERSION     DANBWH010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.285"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..1978
                     /locus_tag="PPY92_002605"
     CDS             <1..1978
                     /locus_tag="PPY92_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDJ2626543.1"
                     /translation="IKNNKKTIMLYLSSDWFAELGFTFFNYHYTAKLIKSSYNLKCLL
                     LKLTYRYLDNQPLNDADIRKLQDIIKIIAKEASMDKKIAQNQYRYAYYGDLRDELEYI
                     YQNVNQRLTLKSVADKLFVSKSNLSSQFHLLMGMGFKKYIDTLKIGKSIEILLTTDST
                     ISNISEHLGFSSSSTYSKMFKSYMDITPNEYRNLSKYNKCLMLKPEPLVGKMVQEVKE
                     IILNYIEHYKNHLTDVIHIDEDKFETPKLFQTVIQINTYTEMKLVFLEGIFKTLLNKN
                     SQVVFFIMPSILKSKNTMSEEEKFTIIKTIIESDLKIAFNINDIETTYFVEEAFMSVF
                     RQISPNELSNHNNYEVHFVFDLSLMEIRTIYRMILKLHNIMLNVKLGLNITCLFEKPS
                     VFKSLVSQIKRLKFDSLIIDNANLSSPYLMGESDELLLKNILHFKNLKQVINELDIEQ
                     EKLIFLNVENHKLLNNKERDLSNSAPLIYKTLSALYHNFDGFGLNIFDNHHTFNAMHL
                     YDKNGFKTTLGLILEKFIEYVSKPKYENSYYSIFDIENYYCLVIYDWRVIESETIMSN
                     FEDSQVYINFKNNVLNDKYLIVIETLDENSGNINHLISKELRDKYEWNPSLLSKIDNY
                     LKPAIEIKEHNFSDNSLNINVTFNALYIIKIGKK"
     gene            2124..>2339
                     /gene="sarX"
                     /locus_tag="PPY92_002606"
     CDS             2124..>2339
                     /gene="sarX"
                     /locus_tag="PPY92_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarX"
                     /protein_id="HDJ2626544.1"
                     /translation="MNTEKLETLLGFYKQYKALSEYIDKKYKLSLNDLAVLDLTMKHC
                     KDEKVLMQSFLKTAMDELDLSRTKLLVS"
BASE COUNT          932 a          276 c          347 g          784 t
ORIGIN      
        1 tattaagaat aataaaaaaa cgatcatgtt gtatttgagt agcgattggt ttgcggaatt
       61 aggctttact ttctttaatt accactatac agcaaagttg attaaatcat cctataattt
      121 gaaatgtcta ctattaaaat tgacttatcg atatcttgat aatcaacctc tcaatgacgc
      181 tgatattaga aaattacagg atattattaa aatcattgca aaagaagcaa gtatggataa
      241 aaagattgcg caaaatcaat atcgatatgc ctattatggt gatttacgtg acgagctcga
      301 atatatttat caaaatgtaa atcaacgatt gacattaaaa agtgtcgctg ataaattatt
      361 tgtctcaaag tcaaatttgt catcacaatt ccacttactt atgggcatgg gttttaaaaa
      421 atatattgat actttgaaaa ttggtaaatc gattgaaatt ctacttacta ctgatagtac
      481 tattagcaac ataagtgagc atttaggttt tagtagtagc tccacttact ctaaaatgtt
      541 taaaagttat atggatataa caccgaatga atatcgtaat ttatcaaaat ataataaatg
      601 tttaatgcta aagccagaac cactagtagg caaaatggtg caagaagtaa aagaaatcat
      661 attgaattat attgaacatt ataaaaacca cctaactgat gttatacata ttgatgaaga
      721 caaatttgaa acacctaaat tgtttcaaac ggttattcaa ataaatactt atacagaaat
      781 gaaattagtt ttcttagaag gaatctttaa aaccttattg aataagaaca gtcaagttgt
      841 ctttttcatc atgccatcga ttctaaaaag taaaaatacc atgtccgaag aagaaaaatt
      901 cacaatcatt aaaacaataa ttgaaagtga tctaaagata gcatttaata taaatgatat
      961 tgaaacaact tattttgttg aagaagcttt tatgagtgtt ttcagacaaa tatctccaaa
     1021 cgaattaagt aatcataata attacgaagt gcattttgtt tttgatttat cattgatgga
     1081 aattagaaca atttatcgaa tgatattaaa attacataac atcatgttga atgtgaaatt
     1141 aggattgaac attacctgtt tatttgaaaa accttcagtt tttaaatcac tagtatcaca
     1201 aataaagcga cttaaattcg attcgttaat aatagataat gcaaatttaa gtagccctta
     1261 tttgatgggg gaaagtgatg agttactatt gaaaaatatt ttgcatttta aaaatttaaa
     1321 acaagtaatt aatgaattgg atattgaaca agaaaagctt atttttctaa atgttgaaaa
     1381 tcataaactg cttaataata aagaacgaga tttaagtaat agtgctccat taatttataa
     1441 gacattaagt gcgctgtatc acaactttga tggctttgga ttaaacattt ttgataatca
     1501 tcatacattt aatgcgatgc atctatatga taaaaatgga tttaaaacaa cactaggtct
     1561 tatattggaa aaatttatcg aatatgtctc gaaaccaaaa tacgaaaaca gttattattc
     1621 tatttttgat atagagaatt attattgcct ggttatttat gattggcgag tgattgagag
     1681 cgagacaatt atgagcaatt ttgaggatag tcaagtttat ataaatttca aaaataatgt
     1741 tttaaacgat aaatatctaa ttgtaataga aacattggac gaaaatagtg gcaacattaa
     1801 tcatttgatt tctaaagaat taagagataa atatgaatgg aaccctagtt tactatctaa
     1861 aattgacaac taccttaaac cagcaatcga gattaaagag cataatttta gtgataattc
     1921 cttgaatatt aacgttactt ttaatgcgtt atacataatt aaaataggaa aaaaataaca
     1981 ccttgatatg tattgcaaaa tttaatttgc atttgttggt aattttgaca acgtattaaa
     2041 aacaaatgag aaagacaatg gcaaaaaaat ttaagataat aatgacagaa gcattgtctt
     2101 tatatatttg ggggtgcaac attttgaata ctgagaaatt agaaacattg cttggcttct
     2161 ataaacaata taaagcatta tctgaatata ttgataaaaa atataagttg tcgctaaatg
     2221 atttagcagt cttagattta acgatgaagc attgcaaaga tgaaaaagta cttatgcaat
     2281 catttttaaa aactgcaatg gatgagctag atttaagtag gacaaaatta ttagtttct
//
LOCUS       DANBWH010000277         2311 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.286, whole genome shotgun sequence.
ACCESSION   DANBWH010000277 DANBWH010000000
VERSION     DANBWH010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.286"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..249
                     /locus_tag="PPY92_002607"
     CDS             <1..249
                     /locus_tag="PPY92_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HDJ2626545.1"
                     /translation="SNEFVFYNASKITINDLSIKLKSAMANDQGITKHDIGLAERAVY
                     KVYFKNGSSKYVDLKTEYKDERVFKATDIKKVDIELKF"
     gene            complement(528..1814)
                     /locus_tag="PPY92_002608"
     CDS             complement(528..1814)
                     /locus_tag="PPY92_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDJ2626546.1"
                     /translation="MNPLAQSLNEQLQQSNATAFTMLSDLGQNMFYPKGILSQSAEAK
                     STTYNATIGMATNKDGKMFASSLDAMFNDLTPDEIFPYAPPQGIEELRDLWQQKMLRD
                     NPELSIDNMSLPIVTNALTHGLSLVGDLFVNQGDTILLPEHNWGNYKLVFNTRNGANL
                     QTYPIFDKDGHYTTDSLVEALQSYNKDKVIMILNYPNNPTGYTPTHKEVTTIVDAIKA
                     LADKGTKVIAVVDDAYYGLFYEDVYTQSLFTALSNLNSNAILPVRLDGATKEFFAWGF
                     RVGFMTFGTSDQTTKEVLEAKVKGLIRSNISSGPLPTQSAVKHVLKNNKQFDKEIEQN
                     IQTLKERYEVTKEVVYADQYHSHWQAYDFNSGYFMAIKVHDVDPEELRKHLIEKYSIG
                     VIALNATDIRIAFSCVEKEDIPHVFDLIAKAIDDLR"
     gene            2014..2112
                     /locus_tag="PPY92_002609"
     CDS             2014..2112
                     /locus_tag="PPY92_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626547.1"
                     /translation="MMWWQEGMMTLITGGLLIIFRLWLELKWKNKK"
BASE COUNT          774 a          333 c          394 g          810 t
ORIGIN      
        1 tcaaatgaat ttgtgttcta taatgcaagc aaaattacaa ttaatgattt aagtataaaa
       61 cttaaatcag caatggcaaa tgatcaaggg ataactaaac atgacatagg acttgctgaa
      121 cgcgcagtgt ataaagtgta ttttaaaaat ggttcgtcaa aatatgtaga cttaaaaact
      181 gagtataaag atgaaagagt atttaaagca actgacatta aaaaggtaga tattgaactt
      241 aaattctaat ctttaatttt ataataaggc atctcacaat cgtggggtgc cttttacatt
      301 tgtagagatg tgatgcttga atgtttgaag tgatttgcca aaagttatat aaatttgtac
      361 caaataaatt aaattttgtt aggagagatg tataaaacta attaggtata gtgtttgaca
      421 agttgctgaa taaaatatat cttatgatac tttgaaagaa ggaataattt taaaaataaa
      481 aaaccaataa tccgagtcat actcatcaga ttattggttg aaattaatta tcttaagtca
      541 tcaatcgctt tagcaattaa atcaaataca tgtgggatat cttctttttc tacacagcta
      601 aacgcaatac gaatatctgt tgcattaagt gcaataacac caattgaata tttttcaatt
      661 aaatgtttac gaagttcttc agggtcaaca tcatgaactt ttattgccat aaagtatcca
      721 gagttaaagt cataggcttg ccaatgtgaa tgatattgat cagcatagac aacttcctta
      781 gtgacttcat agcgttcttt taatgtttga atattttgtt cgatttcttt atcgaattgt
      841 ttattatttt ttaatacatg cttaacagcg ctttgtgttg gaagaggtcc actagaaatg
      901 ttacttcgta taagaccttt tactttggct tctaatactt ctttagttgt ttgatcagac
      961 gttccaaatg tcataaaccc aacacggaat ccccatgcga aaaattcttt tgttgcacca
     1021 tctaaacgaa caggtaatat tgcatttgaa tttaaattag ataatgcagt aaataatgat
     1081 tgagtataca catcttcata gaataaacca tagtatgcgt catccacaac agctataact
     1141 tttgtacctt tatcagctaa tgctttaatt gcatcgacga tggtagtcac ttctttatgc
     1201 gtaggtgtgt aacctgtcgg attattagga taatttaaaa tcataatgac tttatcttta
     1261 ttgtatgatt gtaaagcttc tacaagtgaa tcagtagtat aatgcccgtc tttatcaaag
     1321 ataggatatg tttgaaggtt tgcaccattt ctcgtattga aaacaagttt gtaattaccc
     1381 caattatgct ctggtaacaa gatagtgtca ccttgattta caaataaatc gccaactaaa
     1441 gataaaccat gtgttaatgc attcgtaaca attggtagtg acatgttgtc gattgatagc
     1501 tctggattgt cacgcaacat tttttgttgc cataaatcac gtaattcttc gatgccttgt
     1561 ggtggcgcat aagggaatat ttcatctgga gttaaatcat taaacattgc atctaaagat
     1621 gatgcaaaca tttttccgtc tttgtttgtc gccataccta tagttgcatt atatgttgta
     1681 ctctttgctt cagcagattg agataaaatg ccttttggat aaaacatatt ttgacctaag
     1741 tcagataaca ttgtaaaggc agttgcattt gactgttgaa gttgttcatt taagctttgg
     1801 gctaaaggat tcattattgc ttaacctctc tttaaaaaaa ttgtatgcct atatattatc
     1861 tttaattata ccaaagataa agtgttgttg gtgaattaac gaataatatg taaaatctgc
     1921 tgccaaaata cgaacatatg ttctgttttt atgttagaat aggtgtaggt catccttcag
     1981 tatgatactt tagtcgaacg gaggtgtagt gtaatgatgt ggtggcaaga agggatgatg
     2041 acacttatta caggtggctt actaattatt tttcgacttt ggttagaact gaaatggaaa
     2101 aataaaaaat gatcctacac acataaataa ccccaagtct gctggtacca gacttggggt
     2161 tgtgtagtat aattctgtgg atacacttaa ataatatcat tgaacacaat atatcgcaat
     2221 caataatcat tttgattgaa tggttttgtt gcgctatttt agttgaaagt gattagtacc
     2281 ttgaactttt gaaattcaat aacttattaa a
//
LOCUS       DANBWH010000278         2298 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.287, whole genome shotgun sequence.
ACCESSION   DANBWH010000278 DANBWH010000000
VERSION     DANBWH010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.287"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(50..586)
                     /locus_tag="PPY92_002610"
     CDS             complement(50..586)
                     /locus_tag="PPY92_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDJ2626548.1"
                     /translation="MITVLFGGSRPNGNTAQLTKFALQDLEYQWIDVTQHQFKPIRDV
                     RHTAETITSYDDDYLPILDKILASDTIIFASPVYWYSISAPLKAFIEHWSETLQDKRY
                     PNFKAQMAEKDFRVILVGGDCPKIKAKPAITQMKYSLDFLGATLNGYIIGTAEKPGDI
                     MKDNYALARATEWNSILQ"
     gene            complement(1032..1970)
                     /locus_tag="PPY92_002611"
     CDS             complement(1032..1970)
                     /locus_tag="PPY92_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDJ2626549.1"
                     /translation="MKQFVNLGKSDVEVFPIALGTNAVGGHNLYPNLDEEQGKDVVRQ
                     AINHGINLLDTAYIYGPERSEELVGEVVKEYPREQIKIATKGSHEFDENQEVHQNNQP
                     EYLKQQVENSLKRLQTDYIDLYYIHFPDDNTPKDQAVAALQELKEQGKIKAIGVSNFT
                     LDQLKEANKDGYVDVVQLEYNLLHRENEAVLQYCVDHLITFIPYFPLASGILAGKYDE
                     NTKFSDHRTTRRDFIPGVFEENVRRVKALESLAAAHQTSIANIVLAFYLTRPAIDVII
                     PGAKRAEQVIENIKAADIVLSDDEIQYIDELFPIED"
BASE COUNT          710 a          438 c          352 g          798 t
ORIGIN      
        1 aataaaaata acgaagcaaa aaatcacttc gtttagtatg aggtatgtct tattgcaata
       61 tactattcca ctcagttgca cgtgctaagg catagttgtc tttcatgatg tcaccaggct
      121 tttcagcagt tccaataata taaccattta aagtggcacc taaaaagtct aaactatatt
      181 tcatttgcgt aattgctggc ttcgctttta tttttggaca atctccacca actaaaataa
      241 ctctaaaatc cttttcggcc atttgtgcct taaaattagg atatcgttta tcttgtaatg
      301 tttctgacca atgttcgata aatgctttca atggtgctga aatgctatac caatacactg
      361 gtgatgcaaa aataattgta tcactagcca atattttatc tagaatcggc aaatagtcat
      421 cgtcatatga agtaatagtc tctgctgtat gtctcacgtc acgtatcggt ttaaactgat
      481 gttgtgtcac gtcaatccat tgatactcta aatcttgcaa agcgaatttt gttaattgtg
      541 cagtattacc gtttggtcta ctcccaccaa acaaaacagt aatcatttta gcctaacctc
      601 acttttgatt aataaatatc tgtgtttttc gttacctaat tatactatca taagctttgc
      661 ctaccgaata gtaaaacgct tacaactttt atataaattt gacgaaattt cgtcatgcct
      721 tatataacgt cgtttgtgat gggagtggga cagaaatgat attttcgaaa aatttatttc
      781 gtcgtcccac cccaacttgc acattattgt aagctgactt ttcgtcagct tctgtgttgg
      841 ggcccacacc ccaacttgca ttgcctgtag aatttctttt cgaaattctc tgtgttgggg
      901 cccctgacta ggattgaaaa aagcttgtta caagcgcatt ttcgttcagt caactactgc
      961 caatataact tcgtagagca tagaacattg atttatgtcc cagcctgata tcaccattaa
     1021 atacaattca tttagtcttc aattggaaac aattcatcga tatattgaat ctcatcatct
     1081 gataaaacga tatctgcagc tttaatattt tcaatgactt gttctgcacg ttttgcacca
     1141 ggaataatca catcgatagc tggtctcgtt aaataaaatg ctaatacaat gttcgcaatt
     1201 gaagtttgat gtgctgcagc taggctttcc aaagctttta cgcgacgcac attctcttca
     1261 aaaacaccag gtataaaatc acgacgtgta gtacgatggt cactaaattt agtgttctca
     1321 tcatattttc cagctaaaat accggatgct aatgggaaat aaggaataaa tgtgattagg
     1381 tgatcaacac aatattgcaa tactgcctca ttttcgcggt gcaataaatt atattctaac
     1441 tgtacaacat caacgtaacc atctttattt gcttctttaa gttgatctaa tgtgaaattt
     1501 gatacaccaa tggctttaat cttcccttgt tccttaagct cttgtaatgc tgcaactgct
     1561 tgatctttcg gagtgttgtc atcaggaaaa tgaatataat ataaatcgat ataatcagtt
     1621 tgtagacgtt tcaaactatt ctcaacttgt tgttttaaat attccggttg attgttctga
     1681 tgtacttctt gattttcatc aaattcatga gaccctttcg tagcaatttt aatttgctct
     1741 cgcggatatt ccttaacaac ttctccaacc aattcttctg atcgttctgg cccataaata
     1801 tatgccgtat ctaataaatt aataccatga ttaatggctt gacgaacaac atcttttcct
     1861 tgttcttcat ctaagttcgg atataaatta tgcccaccta ctgcgttcgt cccaagtgcg
     1921 attggaaaca cttcaacatc agatttacct aagtttacaa attgcttcat tagacccagc
     1981 tccttatctt tataactttt ccactatcac actatcatga tttaatacaa cttccaaatg
     2041 ttagaattag ggtttgaatt aaggcggtgt attagaaaaa tgttatgtag aactcagtta
     2101 aaataagtat tgcttaagtt gatatgcata tagcgcgatt gctcgttgac ctttaaatta
     2161 catgtacttt tgaatcagaa attgaaaaac atttaagaac gacaatatta aagccattgc
     2221 aaatttaatt agaaatacta taatgttttg atttgcataa caaagaaatt aaaactgtta
     2281 atattaacat gggatatc
//
LOCUS       DANBWH010000279         2285 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.288, whole genome shotgun sequence.
ACCESSION   DANBWH010000279 DANBWH010000000
VERSION     DANBWH010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.288"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            87..221
                     /locus_tag="PPY92_002612"
     CDS             87..221
                     /locus_tag="PPY92_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626550.1"
                     /translation="MHYLICIKANQRLIFTNSIVDWFYLCMNEQLFDIIKNINDFESI
                     "
     gene            385..1941
                     /locus_tag="PPY92_002613"
     CDS             385..1941
                     /locus_tag="PPY92_002613"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDJ2626551.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIR
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVT
                     DGKDKLRHVYDPTCGSGSLLLRVGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYEN
                     FEIRNDDTLENPAFLGNTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADF
                     AFIQHMVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYG
                     TSIPTCILVFKKCRQQDDNVLFIDASNDFEKGKNQNHLSDAQVERIIDTYKRKATIDK
                     YSYSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAY
                     LKELGVLKDE"
     gene            1934..>2285
                     /locus_tag="PPY92_002614"
     CDS             1934..>2285
                     /locus_tag="PPY92_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDJ2626552.1"
                     /translation="MSNTQTKNVPELRFPGFEGEWEEKKLGNLTTKIGSGKTPKGGSE
                     NYTNKGIPFLRSQNIRNGKLNLNDLVYISKDIDDEMKNSRTYYGDVLLNITGASIGRT
                     AINSIVETHANLNQH"
BASE COUNT          844 a          382 c          437 g          622 t
ORIGIN      
        1 attgcagata atttagataa ataattaaaa cttagacatt cacccaatcc tgacaaaata
       61 tactataact aacatttatt aatatattgc attatttaat atgcatcaaa gccaatcaac
      121 gattgatttt caccaactca attgttgatt ggttttattt atgtatgaat gaacaacttt
      181 ttgacatcat taagaatata aatgattttg aaagcatttg aaagctacaa catttctata
      241 aaatttttca ataacaattg cgccactaaa actcaaaatt tccaccacca acatccaaat
      301 tatcaacatc gcaacataac caaatgttat aataaatcta ttacacaaag agataaatta
      361 cttatgcaaa ggcggaggaa tcacatgtct attactgaaa aacaacgtca gcaacaagct
      421 gaattacata aaaaattatg gtcgattgcg aatgatttaa gagggaacat ggatgcgagt
      481 gaattccgta attacatttt aggcttgatt ttctatcgct tcttatctga aaaagccgaa
      541 caagaatatg cagatgcctt gtcaggtgaa gacatcacgt atcaagaagc atgggcagat
      601 gaagaatatc gtgaagactt aaaagcagaa ttaattgatc aagtcggtta cttcattgag
      661 ccacaagatt tattcagtgc gatgattcgt gaaattgaaa cgcaagattt cgatatagaa
      721 catctagcga cggcgattcg caaagttgaa acatctacat taggtgaaga aagtgaaaat
      781 gactttatcg ggctgttcag cgatatggat ttgagttcaa cgcgactagg taacaatgtc
      841 aaagaacgta ctgctttaat ctctaaagtc atggttaatc ttgacgactt accattcgtt
      901 cacagtgaca tggaaattga tatgttaggt gatgcatatg aattcctaat cgggcgcttt
      961 gcggcgacag cgggtaaaaa agcaggcgag ttctatacac cacaacaagt atctaagata
     1021 ctggcgaaga ttgtcacaga cggtaaagat aaattacgtc acgtgtacga cccaacatgt
     1081 ggttccggtt cattgttgtt acgtgttggt aaagaaacgc aagtgtatcg ttatttcgga
     1141 caagaacgta acaatactac atacaactta gcacgcatga atatgttatt acatgatgtg
     1201 cgttatgaga acttcgagat ccgtaatgat gacacattgg aaaatccagc ctttttaggc
     1261 aatacatttg atgcggttat tgcgaaccca ccatacagtg cgaaatggac agcagattca
     1321 aaatttgaaa atgatgaacg attcagtggt tacggcaagc ttgcgccaaa atccaaagca
     1381 gactttgcct ttattcaaca catggtacat tacctagacg atgaaggtac catggcagtc
     1441 gtactcccac atggtgtatt attccgtggt gccgcagaag gtgtgattcg tcgctattta
     1501 atagaagaaa agaactactt agaagccgtg attgggttac cagccaatat tttctatggg
     1561 acaagtattc caacatgtat tttagtattt aaaaaatgtc gccaacaaga cgacaacgta
     1621 ctatttatcg atgcatccaa tgattttgaa aaaggaaaaa atcaaaacca tttaagcgat
     1681 gcccaagtcg aacgtattat agacacatat aagcgtaaag caacgattga taaatatagc
     1741 tacagcgcga cattacaaga gatcgccgat aacgattaca acttaaacat accgcgatat
     1801 gttgatacat tcgaagaaga agcaccaatt gatttagatc aagtccaaca agatttgaaa
     1861 aatatcgaca aagaaatcgc agaaattgaa caagaaatca atgcatacct gaaagaactt
     1921 ggggtgttga aagatgagta atacacaaac gaaaaatgtg ccagagttga gattcccagg
     1981 atttgagggc gaatgggaag agaagaagtt agggaatctt actaccaaaa taggtagtgg
     2041 aaagactccc aaaggtggaa gtgaaaacta tacaaacaaa ggcataccat ttttaaggag
     2101 tcaaaatatt agaaatggta aattaaatct taatgactta gtttatatta gtaaagatat
     2161 agatgatgag atgaaaaata gtagaacgta ctatggtgat gttcttttaa atattacagg
     2221 agcatcaata ggtagaacag ccattaattc gatagttgaa acgcatgcta atttaaatca
     2281 acatg
//
LOCUS       DANBWH010000280         2284 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.289, whole genome shotgun sequence.
ACCESSION   DANBWH010000280 DANBWH010000000
VERSION     DANBWH010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.289"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..784
                     /locus_tag="PPY92_002615"
     CDS             <1..784
                     /locus_tag="PPY92_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001628779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="coagulase"
                     /protein_id="HDJ2626553.1"
                     /translation="QERPESIPALTSEKNHNQTMALKLKADTEAAKNDVSKRSKRSLN
                     TQNNKSTTQEISEEQKAEYQRKSEALKERFINRQKSKNESVVSLIDDEDDNENDRQLV
                     VSAPSKKPTTPTTYTETTTQVPMPTVERQTQQQIVYKTPKPLAGLNGESHDFTTTHQS
                     PTTSNHTHNNVVEFEETSALPGRKSGSLVGISQIDSSHLTEREKRVIKREHVREAQKL
                     VDNYKDTHSYKDRLNAQQKVNTLSEGHQKRFNKQINKVYNGK"
     gene            1137..2159
                     /gene="emp"
                     /locus_tag="PPY92_002616"
     CDS             1137..2159
                     /gene="emp"
                     /locus_tag="PPY92_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix protein-binding adhesin
                     Emp"
                     /protein_id="HDJ2626554.1"
                     /translation="MKKKLLVLTMSTLFATQLINSNHAKASVTESVDKKFVVPESGIN
                     KIIPAYDEFKNSPKVNVSNLTDNKNFVVSEDKLNKIVDSSAASKIVDKNFAVPESKLG
                     NIVPEYKEINNRVNVATNNPASQQVDKHFVAKGPEVNRFITQNKVNHHFITTQTHYKK
                     VITSYKSTHVHKHVNHAKDSINKHFIVKPSESPRYTHPSQSLIIKHHFAVPGYHAHKF
                     VTPGHASIKINHFCVVPQINSFKVIPPYGHNSHRMHVPSFQNNTTATHQNAKVNKAYD
                     YKYFYSYKVVKGVKKYFSFSQSNGYKIGKPSLNIKNVNYQYAVPSYSPTHYVPEFKGS
                     LPAPRV"
BASE COUNT          938 a          379 c          331 g          636 t
ORIGIN      
        1 acaagaacgt ccagagagta taccagcatt aactagtgaa aaaaatcata atcagactat
       61 ggcattaaag ttaaaagcag atacagaagc tgctaaaaat gacgtatcaa aaagaagtaa
      121 aagaagttta aatactcaaa ataataaatc tacaacacaa gaaatttctg aagaacaaaa
      181 agctgaatat caaagaaagt cagaggcatt aaaagaaaga tttataaaca gacaaaaatc
      241 taaaaatgag tctgtggttt cactaatcga tgacgaagac gacaacgaaa acgacaggca
      301 acttgtggtt tctgcgccat caaagaaacc aacaacaccg actacatata ctgaaacaac
      361 gactcaggta ccaatgccta cagttgagcg tcaaactcag caacaaatcg tttacaaaac
      421 accaaaacca ttagctggat taaatggtga aagtcatgat ttcacaacaa cgcatcaatc
      481 accaacaact tcaaatcata cgcataataa tgttgttgaa tttgaagaaa cgtctgcttt
      541 acctggtaga aaatcaggat cactggttgg tataagtcaa attgattctt ctcatctaac
      601 tgaacgtgag aagcgtgtaa tcaagcgtga acacgttaga gaagctcaaa agttagttga
      661 taattataaa gatacacata gttataaaga ccgattaaat gcacaacaaa aagtaaatac
      721 tttaagtgaa ggtcatcaaa aacgttttaa taaacaaatc aataaagtat acaatggcaa
      781 ataatattaa tgcatggctg caaagcaaat aatgagtttg tcgtaaaaat aacaacattt
      841 taaactagca ataaataata tcaaagtcat catttcaatg atgcaatcta gtatagtcca
      901 cattctaaac aggtgtggac tattactttt ttcactttat attacgaaaa aattattatg
      961 cttaactatc aatatcaata attaatttta agctgaaaaa caataaaaat gttaagacaa
     1021 cgtttacttc aagttaatta ttatactgaa aattctggta tataatgctg ttagtgaata
     1081 taacagggaa attatattgg ttataatatt gagtctatat aaaggagaaa taacagatga
     1141 aaaagaaatt attagtttta actatgagca cgctatttgc tacacaactt atcaattcaa
     1201 atcacgctaa agcatcagtg acagagagtg ttgacaaaaa atttgtagtt ccagaatcag
     1261 gaattaataa aattattcca gcttacgatg aatttaagaa ttcgccaaaa gtaaatgtta
     1321 gtaatttaac tgacaataaa aactttgtag tttctgaaga caaattgaat aagattgtag
     1381 attcatcggc agctagtaaa attgtagata aaaactttgc cgtaccagaa tcaaagttag
     1441 gaaacattgt accagagtac aaagaaatca ataatcgcgt gaatgtagca acaaacaatc
     1501 cagcttcaca acaagttgat aagcattttg ttgctaaagg cccagaagta aatagattta
     1561 ttacgcaaaa caaagtaaac caccacttca ttactacgca aacccactac aagaaagtta
     1621 ttacttcata caaatcaaca catgtacata aacatgtaaa tcatgcaaag gattctatta
     1681 ataaacactt tattgttaaa ccatcagaat cgcctagata tacacatcca tctcaatctt
     1741 taattatcaa gcatcatttt gcagttcctg gatatcacgc gcataaattt gttacaccag
     1801 ggcatgctag cattaaaatt aatcactttt gtgttgtgcc acaaataaat agtttcaagg
     1861 taattccacc atatggtcac aattcacatc gtatgcatgt accaagtttc caaaataaca
     1921 caacagcaac acatcaaaat gctaaagtaa ataaagcata tgactataaa tacttctatt
     1981 cttataaagt agttaaaggt gtgaagaaat atttctcatt ttcacaatca aatggttata
     2041 aaattgggaa accatcatta aatatcaaaa atgtaaatta tcaatatgct gttccaagtt
     2101 atagccctac acactacgtt cctgaattta agggtagctt accagcacca cgagtataaa
     2161 aattggcact aagtttacga gatatgataa atacctatta ttttaaatat agtctacaat
     2221 ctatgtggtt gtaggctgta ttttttgcag tttatcaata aacacccatc aacaaattat
     2281 accg
//
LOCUS       DANBWH010000281         2281 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.290, whole genome shotgun sequence.
ACCESSION   DANBWH010000281 DANBWH010000000
VERSION     DANBWH010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.290"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..293
                     /locus_tag="PPY92_002617"
     CDS             <1..293
                     /locus_tag="PPY92_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HDJ2626555.1"
                     /translation="SLKSYNHGDVSINSNVPSYDAKFKMSNKDENVKQLRSRYNIPTD
                     KAPILKMHIDGDLKGSSVGYKKLEIDFSKEENSELSIVDSLNFQPAKNKDDE"
     gene            312..1061
                     /locus_tag="PPY92_002618"
                     /pseudo
     CDS             312..1061
                     /locus_tag="PPY92_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557596.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
     gene            1059..1850
                     /locus_tag="PPY92_002619"
     CDS             1059..1850
                     /locus_tag="PPY92_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001802015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDJ2626556.1"
                     /translation="MGYLKRLVLYIVIMVMSVFIIGCDKSSDTAENPKEGSKEAQIKK
                     SFSKTLDMYPIKNLENLYDKEGYRDGEFKKGDKGTWTISTDFAKSNKQGEMNSEGMVL
                     HFNRNTRTATGYYTVRTTYDEVDKLAREKKYRVEFKNNKIVLLDKVEDENLKQKIENF
                     KFFGQYADFKDLKNYKNGRISSNENVPYYEAEYKRNNSDGNVKKLREKYPITTKQSPI
                     LKLHIDGDIKGSSVGYKQIEYTFSKEKDDETFMSDFLNFGPSHSK"
     gene            1902..>2281
                     /locus_tag="PPY92_002620"
     CDS             1902..>2281
                     /locus_tag="PPY92_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDJ2626557.1"
                     /translation="MGYFKRVVLYIIVMVVSVFIIGCNKSSDTSEKPKEDSKETQIKK
                     SFAKTLDMYPIKNLEDLYDKEGYRDGEFKKGDKGMWTIYTDFAKSNKQGGLSNEGMVL
                     YLDRNTRTAKGHYFVKTFYNKGKFP"
BASE COUNT          965 a          243 c          451 g          622 t
ORIGIN      
        1 agagtttgaa aagttacaac catggcgacg tttcaattaa tagtaatgtt ccaagttatg
       61 acgcgaaatt taaaatgagt aataaagatg aaaatgttaa gcaattaaga agccgttata
      121 acattcctac tgataaagct ccaatattaa aaatgcatat tgatggggac ttaaaaggca
      181 gttccgttgg atataaaaag ttagaaatag acttttcaaa agaagaaaat agcgaattat
      241 caatagtcga ttcattaaat tttcaacctg ccaaaaataa agatgatgaa tgatagggag
      301 gatgtgaagc gatgggatat tcaaaaaggt ttgcattgta cataagtatc ttgattttaa
      361 tagttatggt agcaggttgt ggtaaaagtg atgaaacaaa agaagattcc aaagaagaac
      421 aaattaaaaa gagctttgcg aaaacgttag atatgtaccc tattaagaat ctcgaggatt
      481 tatacgataa agaaggatac cgtgatggcg aatttaaaaa gggtgataag gggacgtggg
      541 ttctgtattc agctatagta tcacaaccaa aaggcgagag tttaaaatca cgaggaatga
      601 tattaaagtt agatagaaat aagagaactg ctaaaggaag ttatattatt agagaattga
      661 aagaagataa aaatcatgat gttcaaaaaa atgaaaagaa atatccagtg aaattggtga
      721 ataataggat agttttggta aaagatgtta aagacaaaaa gttaaaaaat gaaatagagt
      781 cgtttgaatt attttcacaa tatggaaact ttaatcattt tgatcggaat gagattacta
      841 atatttcata taatcctaat gctcccaatt actctgcaga atataaaatg aagaaaaatg
      901 acagaaacat tcaacagttg aaaaagagat ttaatctaaa aactagcaag acaccaaaat
      961 tattgtttaa gggatctgga gatataaagg ggtcttctgt aggatataag gaaatagaaa
     1021 tcatatttag tagaagtaaa gaagaagcat ttataatgat gggttattta aaaagacttg
     1081 tattgtacat agttattatg gttatgagtg tttttataat aggttgtgat aaatcaagcg
     1141 atactgcaga aaatccaaaa gaaggttcaa aagaagcaca aattaaaaag agtttttcga
     1201 aaacgttaga tatgtatcca attaagaatc tcgagaatct atatgacaaa gaaggatatc
     1261 gtgatggcga atttaaaaaa ggtgataaag ggacttggac aatatcaaca gattttgcta
     1321 aaagcaacaa gcaaggtgaa atgaatagcg aaggtatggt actacatttt aatagaaata
     1381 cgaggacagc aacaggatat tatactgtaa gaacaactta tgatgaagtg gataagttgg
     1441 cacgcgaaaa aaaatatcgt gttgaattta aaaacaataa gatagttcta ttggacaaag
     1501 tagaagatga aaatcttaaa caaaaaatag aaaattttaa atttttcggg caatatgccg
     1561 attttaaaga cttgaaaaat tataaaaatg gaagaatatc tagcaatgaa aatgttcctt
     1621 attatgaagc agagtacaaa aggaataata gtgatggaaa tgtaaaaaaa cttagagaaa
     1681 agtacccaat tacaaccaag cagtctccaa tattaaaact gcatatagac ggcgatatta
     1741 aaggtagctc agttggatat aaacagatag aatacacatt ttctaaggag aaagatgatg
     1801 agacttttat gagtgatttt ttgaacttcg gtccatcaca cagtaaatag taaataaaac
     1861 caagggcata aactaattta aaacaagaga gaagtgaaaa gatgggatat tttaaaagag
     1921 ttgtacttta cataattgtt atggttgtga gtgtttttat aataggttgc aataaatcaa
     1981 gcgatacttc agaaaagcca aaagaagatt caaaagaaac acaaatcaaa aagagctttg
     2041 cgaaaacgtt agatatgtat ccaattaaga atctcgagga cttatatgat aaagaaggat
     2101 accgtgatgg cgaatttaaa aagggtgaca aaggaatgtg gactatatat acagattttg
     2161 ctaaaagtaa taaacaaggt ggattgagta atgaaggtat ggtcttatac ttagatagaa
     2221 atacacggac tgcaaaggga cattattttg ttaagacatt ctataataag ggcaaattcc
     2281 c
//
LOCUS       DANBWH010000282         2277 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.291, whole genome shotgun sequence.
ACCESSION   DANBWH010000282 DANBWH010000000
VERSION     DANBWH010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.291"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            11..187
                     /locus_tag="PPY92_002621"
     CDS             11..187
                     /locus_tag="PPY92_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626558.1"
                     /translation="MKHLTKIFVVLAIILFIIGYYLQATNHESQGIKLLLAAIMFMIC
                     AFISRSNDRRKNSK"
     gene            445..825
                     /locus_tag="PPY92_002622"
     CDS             445..825
                     /locus_tag="PPY92_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDJ2626559.1"
                     /translation="MKIILKNNSDFPIYEQIKQQVKQNILKGHVAPGEHLPSMRELAK
                     DLQVSLITTKRAYEDLEKDGFVTTIRGKGTFVKEQDSSILKEKQFFTIENLVKELVNE
                     AQAIEMSLEELQDILTFIYEEESS"
     gene            822..1718
                     /gene="pmtA"
                     /locus_tag="PPY92_002623"
     CDS             822..1718
                     /gene="pmtA"
                     /locus_tag="PPY92_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtA"
                     /protein_id="HDJ2626560.1"
                     /translation="MNAIELSNVNYSSDQFNLKNISFKVPQGFVTGFIGRNGAGKTTI
                     IRLIMDLYQPQTGVIRVLEEDMALNPIELKNSIGFVYSENYFNERWTTKQLEKMIAPF
                     YRKWDHQVFEFYLEKFDLPINKSIKTFSTGMKMKLSLAVAFSHHAELYIFDEPTSGLD
                     PLARNELLEIIQQELIDENKTIFMSTHIISDLEKIADYIIHLSDGEVILNGSKEQLLQ
                     RYQVVSGATEDLDDELASLLIYEEHKRTGFIGLTEHAQVFKEILGHKVNITTPSIENL
                     MVYLEKRKPKYHENIKLMEEGF"
     gene            1719..>2277
                     /gene="pmtB"
                     /locus_tag="PPY92_002624"
     CDS             1719..>2277
                     /gene="pmtB"
                     /locus_tag="PPY92_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtB"
                     /protein_id="HDJ2626561.1"
                     /translation="MKALLIRNFKLRRYTLIIYVLLLTLYPFYIMLDSTKFFYLLQSF
                     ISPTILIIWILDAGHLFRLNRRLGGNDSYYFYMSLPVSKKQLLNANYITCIVLTLIGT
                     LVISLYAYEADVIEPNSIYFSTAYAFVISNFLSIPIAFSQFTELRRVKVPYGIYVFTI
                     IILVPFLFSIAIVLVNYFVLSQSSFP"
BASE COUNT          796 a          294 c          390 g          797 t
ORIGIN      
        1 gtgaaataaa ttgaaacatc taacaaaaat atttgtagta cttgcaatta ttttatttat
       61 tattggttat tatttacaag caactaatca tgaaagccaa ggtataaaat tattattagc
      121 agcgattatg tttatgattt gtgcttttat aagtagaagt aatgatcgac gtaaaaatag
      181 taaatagaat tgactgttta aaacgattgt ttatgttgac gctacggttg tcacgtagag
      241 attaaagaca ttcgttttag cagtcttttt tattgaagta gacaatgcaa gattaaatgg
      301 tagtgtcatt tcattttatt caactgctag taatacaata ctcagtgtta tttctgaaga
      361 ctggttgata atggacatgc atatttacaa aactgtgtat attgtgtata ttgtatatat
      421 acagaagttg atagggggac gttgatgaaa ataattttaa aaaacaatag tgattttccg
      481 atttatgaac agattaagca acaagtaaaa caaaatattt taaagggaca tgttgctcct
      541 ggagagcatt tgccgtcaat gagagaactt gccaaagatc ttcaagtaag tttgattact
      601 accaaacgtg cttatgaaga tttagagaaa gacggttttg ttacaacaat tagaggaaaa
      661 gggacctttg ttaaggagca agatagttct attttaaaag agaaacaatt ttttaccatt
      721 gaaaatttgg ttaaagaatt ggttaatgaa gcgcaagcca tcgaaatgtc acttgaggaa
      781 cttcaagata ttttaacgtt catttatgag gaggaatcat catgaatgcc atagaattaa
      841 gtaatgttaa ttattcaagt gatcagttta atttaaagaa tatctctttc aaagttcctc
      901 aaggattcgt cactggtttt attggtagaa atggcgctgg taaaaccaca ataataaggt
      961 taattatgga tttgtatcaa ccacaaactg gtgttattag agtattagaa gaggatatgg
     1021 ctctcaatcc gatagagtta aagaatagta ttgggtttgt ttactctgaa aattatttta
     1081 atgagagatg gactacgaag caacttgaaa aaatgattgc ccctttttat cgtaaatggg
     1141 accatcaagt atttgaattt tatcttgaaa agttcgattt accaatcaat aaatcaataa
     1201 aaacattttc gactgggatg aaaatgaaat tatcactagc agttgcattt tcacatcatg
     1261 ctgaattgta tatttttgat gagcctactt caggtcttga tcccttagct agaaatgagc
     1321 tattggagat aattcagcaa gaattaattg atgaaaataa aacaatattc atgtcgacac
     1381 acattatctc agatttagaa aaaatagctg attatatcat tcacttaagt gacggtgaag
     1441 ttatcttaaa tggttcaaaa gaacaactgt tacaaagata tcaagttgtg agtggtgcaa
     1501 ctgaggacct tgatgatgaa ttagcatctc tacttattta tgaagaacat aagcgtacgg
     1561 gatttatagg attaacagaa catgcgcaag tatttaaaga aattttaggt cataaagtga
     1621 atatcactac accttctatt gagaacttaa tggtttatct tgaaaagaga aaacctaaat
     1681 atcatgaaaa catcaaattg atggaagaag gtttttaaat gaaggcattg ttaattagga
     1741 attttaaatt acgacgatat acacttatca tctatgttct attattaacc ttatatcctt
     1801 tttatataat gcttgactca acaaagttct tttacctatt gcaatctttt ataagtccaa
     1861 caatattgat tatttggatt ttagatgctg gtcacttatt tagactcaat cgtagattag
     1921 gtggtaatga ttcatattat ttttatatga gtttaccagt gtcaaaaaaa caattgctta
     1981 atgccaacta tattacatgc attgttttaa cattaattgg aacgcttgtt attagtttat
     2041 atgcttatga agcagatgtg attgaaccaa attcaatcta tttttcaact gcatatgcat
     2101 ttgtcatatc taacttcttg tctataccaa ttgcatttag tcaatttaca gaattgcgta
     2161 gagtcaaagt gccatatggt atatacgtgt ttactattat cattttagtt ccatttttat
     2221 tttcaattgc aatagtgttg gtgaattatt ttgttctaag tcaatcatca ttcccag
//
LOCUS       DANBWH010000283         2262 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.292, whole genome shotgun sequence.
ACCESSION   DANBWH010000283 DANBWH010000000
VERSION     DANBWH010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.292"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            27..377
                     /gene="scb"
                     /locus_tag="PPY92_002625"
     CDS             27..377
                     /gene="scb"
                     /locus_tag="PPY92_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-B"
                     /protein_id="HDJ2626562.1"
                     /translation="MKFKKYILTGTLALLLSSTGIATIEGNKADASSLDKYLTESQFH
                     DKRIAEELRTLLNKSNVYALAAGSLNPYYKRTIMMNEYRAKAALKKNDFVSMADAKVA
                     LEKIYKEIDEIINR"
     gene            739..936
                     /locus_tag="PPY92_002626"
     CDS             739..936
                     /locus_tag="PPY92_002626"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626563.1"
                     /translation="MQVGVGPQQREIGSPIPTDNASWRGPNIEKLDHQFQQTMQVGVG
                     PNTEAGEKSAYKNVQVGGAPT"
     gene            complement(1143..1328)
                     /locus_tag="PPY92_002627"
     CDS             complement(1143..1328)
                     /locus_tag="PPY92_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626564.1"
                     /translation="MNKHINKKATKLLEDYKKVQQRKSEETDRGCLKSVIMLIVLFFL
                     IIFGIVACSTNVNFFFQ"
     gene            complement(1950..2183)
                     /locus_tag="PPY92_002628"
     CDS             complement(1950..2183)
                     /locus_tag="PPY92_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626565.1"
                     /translation="MVFNDWEINVVYSGEHEVVTNEKSLFVIDEFYDVAIAIYYLDGK
                     LKVAHVNYGSDFTIDVANKVFELNIQNPNVDEH"
BASE COUNT          851 a          317 c          334 g          760 t
ORIGIN      
        1 agtatcaatt ttaaggagag tttacaatga aatttaaaaa atatatatta acaggaacat
       61 tagcattact tttatcatca actgggatag caactataga agggaataaa gcagatgcaa
      121 gtagtctgga caaatattta actgaaagtc agtttcatga taaacgcata gcagaagaat
      181 taagaacttt acttaacaaa tcgaatgtat atgcattagc tgcaggaagc ttaaatccat
      241 attataaacg tacgattatg atgaatgaat atagagctaa agcggcactt aagaaaaatg
      301 atttcgtatc aatggctgat gctaaagttg cattagaaaa aatatacaaa gaaattgatg
      361 aaattataaa tagataataa ataaaacagg ttgagacaaa aaatggtctt aacctgtttt
      421 caatttgcat atgtgataaa ttctatatca aaatgcttat gtataatgaa tgacatttaa
      481 aagtagggga gacaaatata aatacaatag ttcctaggat tactctcaaa ataactatat
      541 caattattta ctttgctctt ctatttttta aaatatgtac atgtttaaat aatcaaaagt
      601 gtacattatt aaattatcat ttccagttct agtgctatat tggtagtagt tgactaaatg
      661 aaaataagct tataacaagt tttttcaata ctcgtggggc cccaacacag agaatttcga
      721 aaagaaattc tacaaacaat gcaagttggg gtggggccac aacagagaga aataggatca
      781 ccaattccaa cagacaatgc aagttggcgg ggccccaaca tagagaaatt ggatcaccaa
      841 tttcaacaga caatgcaagt tggggtgggg cccaacacag aagctggcga aaagtcagca
      901 tacaaaaatg tgcaagttgg cggggcccca acatagaagc tggcgaaaag tcagcataca
      961 aaaatgtgca agttggggta ggacatcgat aaaaaaatac tttttcttta gaaattagta
     1021 tttcttatgc atgagtttta ctcatgtatt cattttttaa gtagacatta gctacagcta
     1081 atgataaaga accactacat aataaatcat tagtggttct ttatcatttc tatctcactc
     1141 ttttactgga agaaaaagtt tacgtttgta gaacatgcca caataccaaa aataattaag
     1201 aaaaataaga cgataagcat gatgacactt ttcaaacaac ctctatcagt ttcttccgat
     1261 tttctttgtt gaaccttttt ataatcttca agtagttttg tcgctttttt atttatatgt
     1321 ttattcatga tgttgactcc ttataatata tgtttaattc attaaaatag ttgaaaacat
     1381 gacttgaaat aaagatataa agctaaagta aggatgttca attgtttcaa attttatgaa
     1441 agtataacgt taaatatgtt aatttgatga tgatatttgc tttttatttt ccaaatggaa
     1501 tttacttaaa ctgatgcatt aaaatattaa tgaagcacta gaatacataa atgaatagta
     1561 atggtgcaca gtatagaata attaaggcta tattaagtat aaatatcgtt aactgtaagc
     1621 tatctttagt tttaatataa actattagga taatcgacgt aagaagaatc atatatatta
     1681 atgatgaaga agtccataca aaatccgcat catttgttgt taataatggg actataatta
     1741 atccgaaatt aatcatgcat gctatatata ctataatgtt atacacaatg ttaatttttg
     1801 ttcaccacct tatacttcta ttttaaaaac ttctttataa tgatatattg tttaatgttg
     1861 aaataattag attatctaat tttcatttgc tttacatgta aaaggctata tatagtatgc
     1921 tctttatgat tctaaatgct ttttaatatt taatgctcat caacatttgg attttgaata
     1981 ttcaattcaa aaactttatt agctacgtca attgtaaaat cagaaccata gttgacatga
     2041 gctactttta attttccatc taaataatag attgcgattg caacatcgta aaattcgtca
     2101 atgacaaata aactcttttc gtttgttaca acctcatgct ctcctgagta tacaacgtta
     2161 atttcccaat cattaaaaac catttgttaa cctccttgaa catttaaatt gattcaactt
     2221 aagtttaact tattcataca acttcgtaca atatctagat ga
//
LOCUS       DANBWH010000284         2259 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.293, whole genome shotgun sequence.
ACCESSION   DANBWH010000284 DANBWH010000000
VERSION     DANBWH010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.293"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..115
                     /locus_tag="PPY92_002629"
     CDS             <1..115
                     /locus_tag="PPY92_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626566.1"
                     /translation="SIENMRTYQQRVNNLWIKGVDNVIYCGYQVGKQKINN"
     gene            complement(260..2188)
                     /gene="abc-f"
                     /locus_tag="PPY92_002630"
     CDS             complement(260..2188)
                     /gene="abc-f"
                     /locus_tag="PPY92_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HDJ2626567.1"
                     /translation="MILLQLNHISKSFDGEDIFTDVDFEVKTGERIGIVGRNGAGKST
                     LMKIIAGVENYDSGNVSKIKNLKLGYLTQQMTLNSNATVFEEMSKPFEHIKRMESLIK
                     EETDWLSKHANDYDSDTYKTHMSRYESLSNQFEQLEGYQYESKIKTVLHGLNFSEEDF
                     NKPINDFSGGQKTRLSLAQMLLNEPDLLLLDEPTNHLDLETTKWLEDYLRYFKGAIVI
                     ISHDRYFLDKIVTQIYDVALGDVKRYVGNYEEFIQQRDLYYQKRMQEYESQQAEIKRL
                     ETFVEKNITRASTSGMAKSRRKILEKMERIDKPMLDAKSANIQFGFDRNTGNDVMHVK
                     NLEIGYQTAITKPISIEVSKGDHIAIIGPNGIGKSTLIKTIANQQKALNGDITFGANL
                     QIGYYDQKQAEFKSSKTILDYVWDQYPLMNEKDIRAVLGRFLFVQDDVKKIINDLSGG
                     EKARLQLALLMLQRDNVLILDEPTNHLDIDSKEMLEQALQHFEGTILFVSHDRYFINQ
                     LANKVFDLTIDGGKMYLGDYQYYIEKVEEAEALKAHQEEQSVNVQAHKKSMEQSSYHN
                     QKEQRREQRKLERQISECENEIETLETTILQIDEQLTQPEVYNNPQKANELAIQKQDS
                     EQKLEHAMSKWEELQQKL"
BASE COUNT          664 a          366 c          341 g          888 t
ORIGIN      
        1 gtcaatagag aacatgcgta cttatcaaca aagagtgaat aacttgtgga taaaaggtgt
       61 tgataacgtc atttattgtg gatatcaggt gggaaaacag aaaataaata attgattagt
      121 gttaatttac ttgtggataa ctaggataag tctgtggata gctataatat cagcgttttt
      181 attgtgaata aaaaaaatta aaatttgtaa aaaaataact ctaactatag cgaatttaca
      241 gttagagtta tgtatatttt tatagttttt gttgtaattc ttcccattta gacatagcgt
      301 gttctaattt ttgttcgcta tcttgttttt ggatagctaa ttcgtttgct ttttgtggat
      361 tgttatacac ttctggttgg gttaattgct cgtctatttg taagatagta gtctctaaag
      421 tttctatttc attttcacac tcggatattt gtcgttcaag tttgcgttgt tcacgtcttt
      481 gttctttttg gttatgatac gacgattgtt ccatagactt tttatgtgct tgtacgttaa
      541 ctgactgttc ttcttggtgc gctttcaacg cttcggcttc ctcaactttt tcaatgtaat
      601 attgataatc tcctaaatac atctttccgc catcaattgt taaatcaaat accttgtttg
      661 ctaattggtt aataaagtaa cgatcatggg aaacaaatag tattgttcct tcaaaatgtt
      721 ggagtgcttg ctctaacatt tcttttgaat ctatatcgag gtggttggta ggttcatcta
      781 aaataagtac attgtcacgt tgcaacataa gtagtgctag ttgtaaacgt gctttttcac
      841 caccagataa atcattaatt atctttttaa catcgtcttg tacaaataag aaacgtccaa
      901 gaactgctcg aatatctttt tcattcatta acggatattg atcccacaca taatctaaaa
      961 tcgttttact agatttaaat tctgcttgct tttgatcata ataaccaatt tgtaaatttg
     1021 cgccgaaagt aatatcgcca ttaagcgctt tttgttgatt agcaatagtt ttaattaagg
     1081 tcgattttcc aataccattt ggcccaatga ttgctatatg atcgccttta gagacctcta
     1141 tactgatagg tttggtaatt gcagtttgat aaccgatttc taaatttttt acatgcatga
     1201 cgtcattacc tgtattccgg tcaaagccaa attgaatatt tgcacttttg gcatctaaca
     1261 ttggtttatc aatgcgttcc attttttcta aaatcttacg tctacttttt gccattccac
     1321 ttgttgaagc acgggtaata tttttctcaa caaaagtttc taatcgtttt atttctgctt
     1381 gttgactttc atattcttgc attcgttttt gataatataa atcccgttgc tgtataaatt
     1441 cctcgtaatt accaacatag cgtttgacat cacctaaagc cacatcataa atttgagtaa
     1501 ctattttatc taaaaagtaa cgatcatggc tgatgatgac gattgcacct ttaaaataac
     1561 gtagataatc ttcaagccac tttgtcgttt ctaaatctaa gtggttagta ggttcatcta
     1621 aaagtaataa atcaggttcg tttaatagca tttgagctaa agataaacgt gttttttggc
     1681 cgccgctaaa atcattgata ggtttattga aatcttcttc actaaaattt aacccgtgaa
     1741 gtactgtttt aattttactt tcatattgat atccttctaa ttgttcaaat tgattcgata
     1801 aagattcata acgggacata tgtgttttat atgtatcact atcgtaatca tttgcatgtt
     1861 ttgataacca atctgtttct tctttgatta agctttccat tcgtttaata tgttcaaatg
     1921 gttttgacat ttcttcaaaa actgttgcat tagaatttaa agtcatttgt tgagttaaat
     1981 aacctagttt taagttttta atttttgaca catttccaga atcatagttt tctacaccag
     2041 ctataatttt catcaatgtt gatttaccgg cgccatttct tcctacgata ccaattcgtt
     2101 caccagtttt tacttcaaaa tcaacatcag taaatatatc ttcaccatcg aacgattttg
     2161 atatatgatt gagttgtaaa agtatcattc gatcttcacc tttcgtcttt ctcaaatttt
     2221 attaacttat gaaaaaagtc gtaattatta cattaataa
//
LOCUS       DANBWH010000285         2181 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.294, whole genome shotgun sequence.
ACCESSION   DANBWH010000285 DANBWH010000000
VERSION     DANBWH010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.294"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            50..1606
                     /locus_tag="PPY92_002631"
     CDS             50..1606
                     /locus_tag="PPY92_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDJ2626568.1"
                     /translation="MENTINESEKKKRFKLKMPGAFMILFILTVVAVIATWVIPAGAY
                     SKLSYEPSSQELKIVNPHNQVKKVPGTQQELDKMGVKIKIEQFKSGAINKPVSIPNTY
                     ERLKQHPAGPEQITSSMVEGTIEAVDIMVFILVLGGLIGVVQASGSFESGLLALTKKT
                     KGHEFMLIVFVSILMIIGGTLCGIEEEAVAFYPILVPIFIALGYDSIVSVGAIFLASS
                     VGSTFSTINPFSVVIASNAAGTTFTDGLYWRIGACIVGAIFVISYLYWYCKTIKNDPK
                     ASYSYEDKDAFEQQWSVLKDDDSAHFTLRKKIILTLFVLPFPIMVWGVMTQGWWFPVM
                     ASAFLIFTIIIMFIAGTGKSGLGEKGTVDAFVNGASSLVGVSLIIGLARGINLVLNEG
                     MISDTILHFSSSLVQHMSGPLFIIVLLFIFFCLGFIVPSSSGLAVLSMPIFAPLADTV
                     GIPRFVIVTTYQFGQYAMLFLAPTGLVMATLQMLNMRYSHWFRFVWPVVAFVLIFGGG
                     VLITQVLIYS"
     gene            1913..2140
                     /locus_tag="PPY92_002632"
     CDS             1913..2140
                     /locus_tag="PPY92_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626569.1"
                     /translation="MTHLTKVLDTLTGICVVLLFSKYFVAYANMVFDWNLRWYLLENI
                     PHLPIILFILMFIFGVPSEMIKDRQRKNNGV"
BASE COUNT          687 a          299 c          429 g          766 t
ORIGIN      
        1 aaatctttaa ggtagaaatg ctaggttaaa tgtcgaagga gatgaaaccg tggaaaatac
       61 aattaatgaa agtgaaaaga aaaaacgatt taaattaaaa atgccaggtg catttatgat
      121 tttattcatt ttaacggttg ttgcagttat agcaacatgg gttattcctg ctggtgcata
      181 ttctaaactt tcttacgaac cttcatccca agaactaaag atagttaacc ctcataacca
      241 agtgaaaaag gttccgggta cgcaacagga actagacaaa atgggggtta aaattaagat
      301 tgaacaattt aaatcaggtg caattaataa gccagtatca attccgaata cttatgaaag
      361 attaaagcaa catccagctg gaccagaaca aataacaagt agcatggttg aaggtacgat
      421 agaagcggtc gatatcatgg tattcattct tgtactaggg ggacttattg gcgtagttca
      481 agccagtggt tcttttgaat cgggattgtt agctttaacg aagaaaacaa aagggcatga
      541 atttatgcta attgtgtttg tatcaatact aatgattatc ggcgggacgt tatgtggtat
      601 tgaagaagaa gctgtagcat tctatccgat tttagtccct atatttatag cgttaggata
      661 cgattctatc gtttcagttg gtgccatatt ccttgccagc tctgtcggta gtacattttc
      721 aactattaac ccgttctcgg ttgtaattgc ctctaatgcc gctggtacaa cttttacgga
      781 tggcttgtat tggagaatag gtgcttgtat tgtcggtgcg atttttgtta ttagttattt
      841 atattggtac tgtaaaacaa ttaaaaacga tcctaaagcg tcatattctt atgaagacaa
      901 agatgctttt gaacagcaat ggtctgtatt aaaagatgac gatagtgccc attttacttt
      961 gcgtaagaag ataatcctta cattatttgt actaccattt ccaattatgg tatggggagt
     1021 tatgacgcaa ggttggtggt tcccagttat ggcttcagca tttttaatat ttacaattat
     1081 aataatgttt attgctggga caggtaaatc tggattggga gaaaaaggaa ctgtagatgc
     1141 atttgtcaat ggtgcatcaa gtttagtagg tgtatcttta attattggtt tagctcgagg
     1201 tattaattta gtgttgaatg aaggtatgat ttcagataca atcttacact tttcatcatc
     1261 tttagttcaa catatgagtg gaccattatt tatcatcgta ttactattta ttttcttctg
     1321 tttaggtttt atcgtgccat catcttctgg attagcggta ttatcaatgc ctatctttgc
     1381 accactagct gatacagtag gtataccaag attcgtcatc gttacgacat atcaatttgg
     1441 tcaatatgcg atgttattct tagcgccgac tggacttgtt atggccacac tacaaatgtt
     1501 aaacatgcga tattcacatt ggttccgatt tgtatggccg gtagttgctt ttgtattgat
     1561 tttcggtggc ggagtactaa ttacgcaagt actaatttat tcataatttg aaatgctata
     1621 ttataaaaat actaattggg ttttatgcat ctcgtaggtt tgtagaaata ctaaactaag
     1681 cgaggtgcat tattattttt gattaagaaa ataatgacgg taatgataac actagtaagt
     1741 aattgataca atgctctatt taataatgat atttttaaaa tttgttttta atgtaatgtt
     1801 agatctatgg tatattatat ttatcgtggt aaatatgtat ttgctgtaaa cagtttttgt
     1861 aacacatgac acatatggtt gtgaaaatag catatataaa ggatggctat aaatgacaca
     1921 tttgacaaag gttttagata cactaactgg aatatgcgta gtattattat ttagtaaata
     1981 ttttgtggcg tatgcaaata tggtgtttga ttggaattta agatggtatt tgctagaaaa
     2041 cataccacat ttgccaatta tattatttat tctgatgttt attttcggag taccttctga
     2101 aatgataaaa gataggcaaa ggaaaaataa cggtgtttaa tttatcgata tttagaggtg
     2161 ataaaatttg tcaactatta c
//
LOCUS       DANBWH010000286         2162 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.295, whole genome shotgun sequence.
ACCESSION   DANBWH010000286 DANBWH010000000
VERSION     DANBWH010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.295"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            16..1005
                     /gene="trpS"
                     /locus_tag="PPY92_002633"
     CDS             16..1005
                     /gene="trpS"
                     /locus_tag="PPY92_002633"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDJ2626570.1"
                     /translation="METLFSGIQPSGIPTIGNYIGALKQFVDVQNDYDCYFCIVDQHA
                     ITMPQDRLKLRKQTRQLAAIYLASGIDPDKATLFIQSEVPAHVQAGWMLTTIASVGEL
                     ERMTQYKDKAQKAVEGIPAGLLTYPPLMAADIVLYNTNIVPVGDDQKQHIELTRNLVD
                     RFNSRYNDVLVKPEIRMPKVGGRVMSLQDPTRKMSKSDDNAKNFISLLDEPNVAAKKI
                     KSAVTDSDGIIKFDRDNKPGITNLISIYAGLTDMPIKDIEAKYEGEGYGKFKGDLAEI
                     VKAFLVEFQEKYESFYNSDKLDDILDQGRDKAHKVSFKTVKKMEKAMGLGRKR"
     gene            complement(1047..1928)
                     /locus_tag="PPY92_002634"
     CDS             complement(1047..1928)
                     /locus_tag="PPY92_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDJ2626571.1"
                     /translation="MQNKSKSPFKIALSRFIHNKIAMLSIIFLLIITIVSIIAPLIAP
                     FPVNQQDLLNIKGEMTAQNILGTDSGGRDNFSRLLYAGRISLSIGITSTIGMLLIGIT
                     VGVISGYFGGIVDTLLMRITEFVMLFPFLIFAIVLNAALGDKIKNPYGSAIILVLVII
                     VLSWGGIARLVRGKVLQEKENEYFLAAKSIGTPTYKIILKHLLPNILSVVIVQATLLF
                     AGMIVVESGLSFLGFGISKAIPSWGNMLSDAQEGDVISGKPWIWMPPAIMITLTILSI
                     NFVGEGLKDAFNPRGRR"
     gene            complement(1940..>2162)
                     /locus_tag="PPY92_002635"
     CDS             complement(1940..>2162)
                     /locus_tag="PPY92_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDJ2626572.1"
                     /translation="FLGADIVSILGGAVITETIFSYNGIGKLFLESVIGQDYPLMMAL
                     TLFFSFLGLLGNLISDITYGFIDPRIRSN"
BASE COUNT          797 a          375 c          334 g          656 t
ORIGIN      
        1 aaagtaggca tttaaatgga gacattattt tcaggcatcc aacctagtgg aattcctact
       61 attggaaatt atattggcgc actaaaacaa tttgttgatg tgcaaaatga ctatgattgt
      121 tatttctgta tcgtagatca acatgcaatt acaatgccac aagatcgttt aaaattacgt
      181 aaacagacca gacaattagc agcgatttat ttagcttctg gtatagatcc agacaaagca
      241 acattgttca tacaatctga agtccctgca cacgtacaag caggatggat gttaactacg
      301 attgcttctg ttggagaatt agagcgtatg acgcaataca aagataaagc tcagaaagca
      361 gttgaaggta taccagctgg tctattaaca tatccacctt taatggcagc tgatattgtt
      421 ctttacaata ctaatatcgt tccagttgga gatgaccaaa agcagcatat cgaattgact
      481 cgtaaccttg tagatagatt taatagtcgc tataatgatg tgcttgtgaa acctgaaatt
      541 cgtatgccta aagttggtgg acgtgtcatg agtttacaag acccaacaag aaaaatgagt
      601 aagagtgatg ataatgctaa aaacttcatt tcattattgg acgagccgaa tgttgcagct
      661 aaaaaaatta aaagcgcagt aactgattca gatggtatta ttaaatttga tcgtgacaac
      721 aagccaggta taacaaattt aatttcaata tacgctggat taaccgacat gccaattaaa
      781 gatattgagg caaaatatga aggggaaggt tatggtaaat ttaaaggtga ccttgctgaa
      841 atagttaaag catttttagt agaattccaa gaaaaatacg aaagtttcta taactcagat
      901 aaacttgatg atattttaga tcaaggtaga gataaagcac acaaagtttc atttaaaact
      961 gtcaaaaaaa tggaaaaagc gatgggatta ggacgtaaga gataaaataa agaggtacta
     1021 gaattaacta gtgcctcttt tattatttaa cgtctacctc taggattaaa agcatcttta
     1081 agcccttccc ctacaaagtt tatacttaat atagttaatg taatcattat agcaggtggc
     1141 atccatatcc acggtttacc acttataaca tccccttctt gagcatcact caacatatta
     1201 ccccaagatg gtattgcttt actaattccg aatcctaaaa agctcaatcc tgattccact
     1261 acaatcatac cggcaaataa caatgttgct tgtacgataa ctacacttaa tatattcggc
     1321 aaaagatgtt tcaaaataat tttatatgtg ggtgtaccaa ttgattttgc tgccaaaaag
     1381 tattcatttt ctttttcttg aagtacttta ccacgaacaa gtcttgcaat acctccccaa
     1441 cttaatacga taataactag aacaagaatt atggcagatc cataaggatt tttaatttta
     1501 tctccaagtg cagcatttaa tacaattgca aatattaaaa atggaaataa cataacaaat
     1561 tcggttattc tcattaataa tgtatcaaca atacctccaa aataaccaga aatcactcca
     1621 actgtaattc caatcaaaag cattcctatt gtagatgtaa ttccaatgga taatgaaata
     1681 cgacctgcat ataacaaacg actaaagtta tccctaccac cagagtctgt accaagaatg
     1741 ttttgtgctg tcatttcacc ttttatattt aataaatctt gttggttcac tggaaaagga
     1801 gctattaatg gcgctataat tgatacaata gttatgatta ataaaaaaat aatcgataac
     1861 attgcaattt tattatgaat aaatctagac aatgcaattt taaaaggcga ttttgactta
     1921 ttttgcattc caatcccccc tagttacttc taattcttgg atctataaat ccataagtaa
     1981 tatcagaaat caaattaccc aataaaccta aaaatgagaa aaataacgtt aatgccatca
     2041 ttaatggata gtcttgacct attaccgatt ctaaaaataa tttaccgata ccgttatatg
     2101 aaaagatagt ctcagtaatc acagctccac ctaaaatact tacaatatca gcacctaaaa
     2161 at
//
LOCUS       DANBWH010000287         2129 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.296, whole genome shotgun sequence.
ACCESSION   DANBWH010000287 DANBWH010000000
VERSION     DANBWH010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.296"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..1463
                     /locus_tag="PPY92_002636"
     CDS             <1..1463
                     /locus_tag="PPY92_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002448877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="poly(glycerol-phosphate)
                     alpha-glucosyltransferase"
                     /protein_id="HDJ2626573.1"
                     /translation="LALKFKKKSGEFPEWIKLDIVTSTEKILFKELKKTLINTRRNYV
                     DFGIAFDSQWNFAVLPEEINANAFVRPDNTTKELFLSEKNINNYLRKYTTNKKAFSSE
                     FYNEKEVIKFYTQGFFIGDEEVHELYSEGYKKGLRKVNDLNNEIDQLIESSTNFLQNM
                     LLDTGKYIYGYFPHFDNEIGFYNVLRHSSSTYALIEGLSYLGKSLQPVEKAIDYIILN
                     QLFEIGDKAYIFDDTEEANEIKLGQNASFIFAVCEYLKHEDNPKFLESAQKVAKGILS
                     MIDEDTYETTHLLNYPDLSVKEKFRIIYYDGEAALALLRLYQKDENELWLKTVENLMD
                     RFIEKKYWQYHDHWLGYCTNELVQINPQDKYFEFGIKNVNNYLDYIKNRETTFPTFLE
                     MLMATYRLVQKAKDTGREELVNNLIDEQYLIDVINIRADYQRVGFFYPEIAMYFKNPS
                     RILGSFFIKHHGYRVRIDDIEHYLSGYVQYQLAFNR"
     gene            complement(1623..>2129)
                     /locus_tag="PPY92_002637"
     CDS             complement(1623..>2129)
                     /locus_tag="PPY92_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDJ2626574.1"
                     /translation="KQLNHQIELVSKLIKEFPNIQLHLYGFGKEEEKYKQLITEYNLE
                     NNVFLRGFRRNLSAEIQDAYMSLITSNMEGFNLGLLETITEGIPPVGYNSKYGPSELI
                     LNNENGYLINKNDKDELYNRVRNLLLDKTLRDTFSQECIKHSKAFSSKIVMKLWEDRF
                     STINSSYE"
BASE COUNT          805 a          268 c          313 g          743 t
ORIGIN      
        1 aattggcact taaatttaaa aagaaatctg gggaatttcc tgagtggatt aaattagata
       61 ttgtaacctc gactgaaaaa atattattta aagaattaaa aaaaacactg atcaatacaa
      121 gaagaaatta tgtagatttt ggtatagcat ttgatagtca atggaatttt gcagtattac
      181 ctgaagaaat taacgcgaat gcttttgttc gaccagataa tactactaaa gagttatttc
      241 tttcggaaaa aaacatcaat aattatttac gcaaatatac tacaaataaa aaggcatttt
      301 ctagtgagtt ctataatgaa aaagaagtta ttaaattcta tacacaaggt ttctttatag
      361 gtgatgaaga agtacatgaa ctatatagcg aaggctataa aaaaggttta agaaaagtaa
      421 atgatttaaa taatgaaatt gatcaattaa ttgaaagtag tactaatttc ttacaaaata
      481 tgttgttaga tactggtaaa tatatttatg gatattttcc tcattttgat aatgaaattg
      541 gattctacaa tgttttaaga cactcttctt ctacttatgc cttaattgag ggtctgtcct
      601 atttaggtaa aagcttacaa cctgttgaaa aagcaattga ttatatcatt ttaaatcaac
      661 tgtttgagat tggtgataaa gcctatatct ttgatgatac agaggaagca aatgaaatca
      721 aattaggaca aaatgcttca tttatattcg cagtttgtga atatttaaag catgaagata
      781 atcctaaatt tcttgagtca gctcagaaag tggctaaagg tattctttca atgatagatg
      841 aagatacata tgagacaact cacttattga attatcctga tttaagcgta aaagagaagt
      901 ttagaattat ttattacgat ggtgaagcag cgcttgcttt attgagattg tatcaaaagg
      961 atgaaaatga actatggcta aaaactgtag aaaatttgat ggaccgtttc attgagaaga
     1021 aatattggca atatcatgat cattggttag gatattgtac gaatgaatta gttcaaatta
     1081 atccacaaga caaatatttt gaatttggaa tcaaaaatgt gaataattac ttagattata
     1141 ttaaaaatcg tgaaacaaca tttccaacat tcttagaaat gttaatggcc acatatagat
     1201 tagttcaaaa agcgaaagat acgggtcgcg aggaattggt aaacaattta atagatgaac
     1261 aatatttgat agatgtaatt aatattagag cagattatca aagagttggc ttcttttatc
     1321 ctgaaattgc tatgtatttt aaaaacccat caagaatact aggaagtttc tttattaaac
     1381 accacggtta tcgtgttcga attgatgata tcgaacatta tttatctgga tatgtacaat
     1441 atcagcttgc atttaatagg taaaataagt atttgaaatt taaattaatg acttacaaaa
     1501 aatggttgat ttacataaaa acgaaatcaa ccatttttaa tttgctcttt aataattatt
     1561 tttcatataa ctaaaattct aatttcatca agaataactg aaaaaactaa acaacatgtg
     1621 agctattcat aacttgaatt aattgtagaa aatctatctt cccataactt cataacaatt
     1681 ttacttgaaa aagctttaga gtgcttgata cattcttgag aaaaagtatc tcttaaagtc
     1741 ttatctaata ataggtttcg cactcgatta tataattcat ctttatcatt tttgttaatt
     1801 aaataaccat tttcattatt taatattaat tcggaaggac catacttcga attatagcca
     1861 actggaggta tgccttcagt tatagtttct aataaaccta aattaaatcc ttccatatta
     1921 ctagtaatta gagacatata agcatcttga atctcagcac ttaaatttct tctaaaccct
     1981 cgtaaaaaca cattgttttc taaattatat tcagtgatta gttgcttgta tttttcttct
     2041 tctttaccaa acccatacaa atgtagttgg atgttaggaa attctttaat taatttagaa
     2101 actaattcaa tttgatgatt caactgttt
//
LOCUS       DANBWH010000288         2120 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.297, whole genome shotgun sequence.
ACCESSION   DANBWH010000288 DANBWH010000000
VERSION     DANBWH010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.297"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(247..669)
                     /locus_tag="PPY92_002638"
     CDS             complement(247..669)
                     /locus_tag="PPY92_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDJ2626575.1"
                     /translation="MAIHYETKATNVGGRKGHVYTDDRALDIDIVPPAQADGKATNPE
                     QLFAAGYASCFNGAFDLILKQNKVRDAHPEVTLTVRLEDDSDSESPKLSVSIDATIKN
                     VISQEEAEKYLQMAHEFCPYSKATQGNINVDLNVNVVD"
     gene            817..1533
                     /locus_tag="PPY92_002639"
     CDS             817..1533
                     /locus_tag="PPY92_002639"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDJ2626576.1"
                     /translation="MTHVEVVATIAPQLSIEETLIQKINHRIDAIDVLELRIDQIENV
                     TVDQVAEMITKLKVMQDSFKLLVTYRTKLQGGYGQFINDLYLNLISDLANINGIDMID
                     IEWQADIDIEKHQRIIKHLQQYNKEVVISHHNFESTPPLDELQFIFFKMQKFNPEYVK
                     LAVMPHNKNDVLNLLQAMSTFSDTMDCKVVGISMSKLGLISRTAQGVFGGALTYGCIG
                     EPQAPGQIDVTDLKAQVTLY"
     gene            1616..>2120
                     /locus_tag="PPY92_002640"
     CDS             1616..>2120
                     /locus_tag="PPY92_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDJ2626577.1"
                     /translation="MELQQAIANRRSVKKFKRDMHIDDALLYQAIEKAADAPNHGMRE
                     PWRVVHVPKDRLGDMSKDISKFAFPNELDKQQCHYDAVTKLGGMLLLILKTDPRQRQN
                     DENYFAFGAYAQNLMLLLYEAGIGTCWKSPLYIYDPKVRKTLGIKKDEVLAGFLYLTD
                     LEEDMPKA"
BASE COUNT          739 a          310 c          387 g          684 t
ORIGIN      
        1 caggaaatta tggaattggt tattgatcga cactggactt ttatttggag acgttatgcg
       61 aagaagtcaa gccagtattg gaaccggtca catttatgat gactaaggga caatcatttt
      121 cagttaaata ttgccagaaa tttcagtcac gaatagaaaa aataaccaac caaaaatttt
      181 tggttggcaa atttgaattt tatgtgaatt ttattaataa cgttgaataa tctctttaag
      241 ttaatgctaa tctacaacat ttacatttaa atcgacatta atatttcctt gagtcgcttt
      301 tgaatatgga caaaattcat gagccatttg taaatatttt tcagcttctt cttgagatat
      361 aacattttta attgtcgcat caattgaaac acttaattta ggactttctg agtctgaatc
      421 atcttctagt ctcactgtta gtgttacttc tggatgagca tcacgcactt tgttttgctt
      481 taaaattagg tcgaaagcac cgttgaagca agatgcataa cctgctgcaa ataattgttc
      541 ggggttagta gctttaccat ctgcttgagc aggcggaacg atatcaatat ctaacgctcg
      601 atcatcagta taaacatgtc ctttacgtcc gccaacattc gtcgctttag tttcataatg
      661 tattgccatt cttataacct cctaatttaa aatacattta tgctttaccc attcgaaaat
      721 accttaatca ttttcattta tagcattgtt tgattgaagg ataaaaagtt gttgtttaca
      781 ataaaaataa tgagtatctg aaatgaggga tttactatga cacatgtgga agtagtagcg
      841 actatcgcgc cacaattatc tatcgaagaa actttaattc aaaaaattaa tcatcgtatt
      901 gatgcaatag acgtattaga attacgaatt gatcaaattg aaaatgtcac agttgatcaa
      961 gtggcagaaa tgattacaaa gctgaaggtt atgcaagatt cattcaaatt attagttacg
     1021 tatcgtacaa agttacaagg tggctatggg caatttataa atgacttgta tcttaattta
     1081 atatcagact tagcaaatat caatggcata gatatgattg atatagaatg gcaagcagat
     1141 attgacattg aaaaacatca acgaatcatt aaacacttgc aacagtataa taaagaggtg
     1201 gttatatcac atcataattt cgaaagtacg cctccattag atgaattgca atttatattt
     1261 tttaaaatgc aaaaattcaa cccagaatac gttaaattag cagtaatgcc acataataaa
     1321 aatgatgtgt taaatttatt gcaggcaatg tctacatttt cagatactat ggactgcaaa
     1381 gttgttggta tttcaatgtc taaacttgga ctaataagta gaacggctca aggcgttttt
     1441 ggtggtgcat tgacttatgg ttgtatcgga gaaccacaag ctccaggaca gattgatgtt
     1501 actgatttaa aagcacaagt gactttatac taataaaatg aattaggttg cttttgtgta
     1561 agtgctgtct tataattgag aagtattatc attgttcgtg gaggagcgat tttagatgga
     1621 attacaacaa gcaatagcta atagaagaag tgtgaaaaaa tttaaaagag atatgcacat
     1681 agatgacgca ttgctatatc aagcaattga gaaagctgct gatgctccaa atcacggaat
     1741 gagggaacca tggagagttg tgcatgttcc gaaagacaga ttaggagata tgagtaagga
     1801 tatttctaaa tttgcatttc ctaatgaatt agataagcaa caatgtcatt atgatgcagt
     1861 aacgaaacta ggtggcatgt tattgcttat tttaaaaaca gatccaagac aacgtcaaaa
     1921 tgatgaaaac tactttgcat ttggtgcata tgcacaaaat cttatgttgt tactttatga
     1981 agcggggata ggtacatgtt ggaaatcgcc attatatatc tatgatccta aagtaagaaa
     2041 aacacttggt ataaagaaag atgaagttct tgctggattc ttatatttaa cggatttaga
     2101 agaagatatg cctaaagcac
//
LOCUS       DANBWH010000289         2115 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.298, whole genome shotgun sequence.
ACCESSION   DANBWH010000289 DANBWH010000000
VERSION     DANBWH010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.298"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            46..264
                     /locus_tag="PPY92_002641"
     CDS             46..264
                     /locus_tag="PPY92_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626578.1"
                     /translation="MENQKQGNGLKIATWVFIVLTVVTPLFGIGSIVCSINYKKYDAE
                     KGSKLLQIAIIVTIIAFVLNLLAYLGLR"
     gene            396..896
                     /locus_tag="PPY92_002642"
     CDS             396..896
                     /locus_tag="PPY92_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDJ2626579.1"
                     /translation="MTFEEKLSQMYNEIANKISSMIPVEWEKVYTMAYIDDEGGEVFY
                     YYTEPGSNELYYYTSVLNKYDISESEFMDSAYELYKQFQNLRNIFKEEGYEPWTSCEF
                     DFTKEGELKVSFDYIDWINTEFDQLGRQNYYMYKKFGVIPEMEYEMEEVKEIEQYIKE
                     QEEAEQ"
     gene            907..1407
                     /locus_tag="PPY92_002643"
     CDS             907..1407
                     /locus_tag="PPY92_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDJ2626580.1"
                     /translation="MTFEEKISKLYNEIANEISSMIPVEWEKVYTMAYIDDGGGEVFF
                     NYTKPGSDDLNYYTDIPKEYNISVQVFDDLWMDLYDLFEELRDLFKEEDLEPWTSCEF
                     DFTSEGKLKVSFDYIDWINTEFDQLGRENYYMYKKFGVIPEMEYEMEEVKEIEQYIKE
                     QDEAEL"
     gene            1418..1903
                     /locus_tag="PPY92_002644"
     CDS             1418..1903
                     /locus_tag="PPY92_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDJ2626581.1"
                     /translation="MIFEEKLSQMYNEIANEISGMIPVEWEKVYTIAYVDDEGGEVVF
                     NYTKPGSDELNYYTYIPREYNVSEKVFYDLWTDLYRLFKKLRNAFKEDLEPWTSCEFD
                     FTREGNLKVSFDYIDWIKLGFGPSGKENYYMYKKFGVLPETEYEMEEIREVEKYVKDQ
                     E"
BASE COUNT          839 a          194 c          428 g          654 t
ORIGIN      
        1 agttaaataa attgaaagac aaaataatga gtaggagtta atgagatgga aaaccaaaaa
       61 caaggcaatg gcttaaaaat tgcaacatgg gtatttattg tattaacagt agttacaccg
      121 ctatttggta ttggaagtat tgtttgtagt attaattata aaaaatacga tgctgaaaaa
      181 ggttcgaagt tattgcaaat tgcaattatc gtaacaataa ttgcttttgt tttaaattta
      241 ttagcatatt taggtttaag ataagtaaaa cgaatttgaa gaagcattaa gcgacatttg
      301 ggtgttgttt aatgcttttt atttaacgga aataagtgta gagaataaat taatagttta
      361 atcaagagat atttcgaaca cataggggcg ataacatgac tttcgaagaa aaattaagtc
      421 aaatgtacaa cgagattgcg aataagatta gtagcatgat accagtagag tgggaaaaag
      481 tatatacaat ggcttatata gatgatgaag gtggagaagt gttctactat tacacagaac
      541 ctggaagcaa tgaattatac tactatacta gtgtattaaa taaatatgat atatcggaat
      601 cagaatttat ggactcagcg tatgagttgt ataaacaatt tcaaaattta agaaatatat
      661 ttaaagaaga aggatatgaa ccatggacat catgcgaatt tgattttaca aaagaaggtg
      721 aattaaaagt ttcatttgat tatatagatt ggatcaatac agagtttgat caattgggcc
      781 gtcaaaatta ttatatgtac aaaaaatttg gggttatacc agaaatggaa tatgaaatgg
      841 aagaagttaa agaaatcgaa caatatatta aagagcaaga agaagctgaa caatagaggt
      901 gataacatga ctttcgaaga gaaaataagc aaattatata atgagattgc gaatgagatt
      961 agcagtatga taccggtaga gtgggaaaaa gtatatacaa tggcttatat agatgatgga
     1021 ggaggtgaag tattctttaa ttatactaaa ccaggtagtg atgacttgaa ttattacacc
     1081 gatataccta aggagtataa catttctgtg caagtatttg atgatttatg gatggattta
     1141 tatgatttgt ttgaggagtt aagagattta tttaaagaag aagatttaga accatggaca
     1201 tcatgtgaat ttgactttac aagcgaaggt aaattaaaag tttcatttga ttatattgat
     1261 tggataaata cagagtttga tcaattaggc cgtgaaaatt attatatgta taaaaaattt
     1321 ggggttatac cagaaatgga atacgaaatg gaagaagtta aagaaatcga gcaatatatt
     1381 aaagagcaag atgaagctga actatagggg cgataatatg attttcgaag aaaaactaag
     1441 tcaaatgtac aatgaaattg caaatgaaat cagtgggatg ataccagtag agtgggaaaa
     1501 agtatataca attgcttatg tagatgatga aggtggagaa gtcgttttta attatactaa
     1561 accaggtagt gatgaattga attattacac atatatccct agagagtata atgtctctga
     1621 aaaagtattt tatgatttgt ggacggattt atatagattg tttaagaagt taagaaacgc
     1681 atttaaagaa gatttagaac catggacatc atgtgaattt gattttacaa gagagggtaa
     1741 tttgaaagta tcgtttgatt atatagattg gattaaatta ggttttggtc catcaggaaa
     1801 ggaaaactac tatatgtata aaaagtttgg tgttttacca gaaacggaat atgaaatgga
     1861 agaaattcga gaagtagaga agtatgttaa agaccaagag tagcatatat gttatagaag
     1921 gctgtgcaaa atcaccctcg ttttacattt gactcaaaga agaaggtaaa cgataagatt
     1981 atttgcaact taaaaagttt attagcttct cggtatccat acatcatatg tataaatgag
     2041 tttaactaat taataaatca cgatataaat ttaaaattgc cattaattga aaaggttatt
     2101 gtagtgtatt ttata
//
LOCUS       DANBWH010000290         2115 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.299, whole genome shotgun sequence.
ACCESSION   DANBWH010000290 DANBWH010000000
VERSION     DANBWH010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.299"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            77..>2115
                     /locus_tag="PPY92_002645"
     CDS             77..>2115
                     /locus_tag="PPY92_002645"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019142.1,HMM:NF037833.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_089205030.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HDJ2626582.1"
                     /translation="MKKKLDFLPNKLNKYSIRRFTVGTASILVGSTLLFGIGNEAHAA
                     EEHQKSNTTENVADSNASEAPTKEEAPSNEATSEAPTKEEVPSNEATSEAPTKEEAPS
                     NEATSEAPTKEEAPSNEATSEASTKEEAPAAEETDKATEEAPKTEETDKATKEEAPAV
                     EETSKAATEEAPKAEETDKATEEAPKTEETSKAATEKAPKAEETNKVETEEAPAAEET
                     NKAATEETPAVEDTNAKSNSNAQPSETERTQVVDTVAKDLYKKSEVTEAEKAEIEKVL
                     PKDISNLSNEEIKKIALSEVLKETANKENAQPRATFRSVSSNARTTNVNYSATALRAA
                     AQDTVTKKGTGNFTAHGDIIHKTYKEEFPNEGKLTAFNTNFNPDTGTKGALEYNDKID
                     FNKDFTITVPVANNNQGNTTGADGWGFMFTQGNGQDFLNQGGILRDKGMANASGFKID
                     TAYNNVNGKVDKLDADKTNNLSQIGAAKVGYGTFVKNGADGVTNQVGQNALNTKDKPV
                     NKIIYADNTTNHLDGQFHGQRLNDVVLNYDAATSTITATYAGKTWKATTDDLGIDKSQ
                     KYNFLITSSHMQNRYSNGIMRTNLEGVTITTPQADLIDDVEVTKQPIPHKTIREFDPT
                     LEPGSPDVIVQKGEDGEKTTTTPTKVDPDTGDVVERGEPTTEVTKNPVDEIVHFAP"
BASE COUNT          925 a          356 c          398 g          436 t
ORIGIN      
        1 gaaataattg tacttctgct aattttataa tgtagggttt agaattctat ataaatttag
       61 aaatggagtg tttttgttga aaaagaaatt ggacttttta cctaataagt tgaataagta
      121 ttcaattaga agatttacag taggtacggc atctatatta gtaggatcta ctctattatt
      181 tggtattggg aatgaggcac atgcagcaga agagcatcaa aaatcaaaca ctacagaaaa
      241 tgtagctgac agtaatgcaa gcgaagcacc aacaaaagaa gaagcgccaa gcaatgaagc
      301 aacaagcgaa gcaccaacaa aagaagaagt gccaagcaat gaagcaacaa gcgaagcacc
      361 aacaaaagaa gaagcgccaa gcaatgaagc aacaagtgag gcaccaacaa aagaagaagc
      421 gccaagcaat gaagcaacaa gtgaggcatc aacaaaagaa gaagcgccag cagctgaaga
      481 aacagacaaa gcaacagaag aagcaccaaa aactgaagaa acagacaaag caacaaaaga
      541 agaagcgcca gcagttgaag aaacaagcaa agcagcaaca gaagaagcgc caaaagctga
      601 agaaacagac aaagcaacag aagaagcacc aaaaactgaa gaaacaagca aagcagcaac
      661 agaaaaagca ccaaaagctg aagaaacaaa caaagtagaa acagaagaag cgccagcagc
      721 tgaagaaaca aacaaagcag caacagaaga aacaccagca gttgaagaca caaatgctaa
      781 gagcaattca aatgctcaac catcagaaac tgagagaact caagttgtag atacagttgc
      841 taaagattta tataaaaaat ctgaagttac agaagcagaa aaagctgaaa ttgaaaaagt
      901 attaccaaaa gatatttcaa acttatctaa tgaagaaatt aaaaaaatag ctttaagtga
      961 agtacttaaa gaaacagcta acaaagaaaa cgcacaacca agagcaacat tccgttcagt
     1021 aagcagcaat gctagaacaa caaatgttaa ctattcagca acagcattaa gagcagctgc
     1081 acaagacaca gttactaaaa aaggaactgg taactttact gcgcatggag atataatcca
     1141 taaaacttat aaagaagaat tccctaatga aggtaagcta actgcattca atacaaactt
     1201 caatcctgat acaggaacta aaggcgcatt agaatataat gataaaatag attttaataa
     1261 agactttaca attactgttc cagtagcaaa caacaaccaa ggcaatacaa caggagcaga
     1321 tggctggggc ttcatgttta ctcaagggaa tggccaagac ttcttaaacc aaggtggtat
     1381 tttaagagac aaaggtatgg caaatgcatc tggttttaaa attgatacgg catataataa
     1441 tgttaatggt aaagtcgata aactcgatgc agataaaaca aacaatctaa gtcaaattgg
     1501 cgcagcaaaa gttggttacg gtacatttgt taaaaatggt gcagatggtg tgactaacca
     1561 agttggtcaa aatgccctaa atacaaaaga taaacctgta aataaaataa tttatgcaga
     1621 taatacaact aatcatcttg atggtcaatt ccatggccaa agattaaatg atgtagtatt
     1681 aaattatgat gcagcaacaa gtacaataac tgctacatat gcaggaaaaa catggaaagc
     1741 tactacagat gatttaggaa ttgataaatc acaaaaatat aatttcttaa ttacttcaag
     1801 tcatatgcaa aatagatatt ctaatggaat tatgagaaca aatcttgaag gtgtaacaat
     1861 tacaacgcct caagctgatt taattgatga tgtggaagta acgaaacaac caattcctca
     1921 taaaactatt cgtgagtttg atccaactct agaaccaggc tcacctgatg ttattgtaca
     1981 aaaaggtgaa gatggagaga aaacaacaac tacaccaact aaagttgacc ctgatacagg
     2041 agatgtagtt gaacgtggtg aaccaacaac agaagttaca aaaaatccag ttgacgagat
     2101 tgtacacttt gcacc
//
LOCUS       DANBWH010000291         2112 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.300, whole genome shotgun sequence.
ACCESSION   DANBWH010000291 DANBWH010000000
VERSION     DANBWH010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.300"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(161..1039)
                     /locus_tag="PPY92_002646"
     CDS             complement(161..1039)
                     /locus_tag="PPY92_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HDJ2626583.1"
                     /translation="MLNIQDVSHLSKKEQKAYNRFVESVENGNLPVLPCIEMDLKEMQ
                     EETLSQSKIGGMPFLKSFKDIPLDENNVPMVLLAQINLNDLPEQQELFPVNEGILQFW
                     ISSEDQMYGMSENLKGNNINSRLVYIKEPITDLSLENIQVHLKSLDADNEDIPFSGAF
                     SIEFRLSKQTITCTDYKYDEDVLALWNKVNPSFALKSMFGGYDELMEPVCNTFTAKEP
                     FNQLGGYPYFDQIDPRTNDQELKMYDRVLLQIDSTRDGNSSIIWGDLGIANILVKSTD
                     LEAMKFDDYMYSWDCS"
     gene            complement(1218..1598)
                     /gene="gcvH"
                     /locus_tag="PPY92_002647"
     CDS             complement(1218..1598)
                     /gene="gcvH"
                     /locus_tag="PPY92_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDJ2626584.1"
                     /translation="MAVPNELKYSKEHEWVKVEGNVATIGITEYAQSELGDIVFVELP
                     ETDDEINEGDTFGSVESVKTVSELYAPISGKVVEVNEELEDSPEFVNESPYEKAWMVK
                     VEISDESQLEALLTAEKYSEMIGE"
     gene            complement(1756..2112)
                     /locus_tag="PPY92_002648"
     CDS             complement(1756..2112)
                     /locus_tag="PPY92_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDJ2626585.1"
                     /translation="MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKT
                     IIANTGVEINKLFNTHGAKYRELDLKNKLQTLSDDEKLELLSSDGMLVKRPLAVMGDK
                     ITLGFKEDQYKETWLA"
BASE COUNT          659 a          405 c          262 g          786 t
ORIGIN      
        1 tgggccccaa caaagagaaa ttggattctc aatttctaca gacaatgcaa gttggggtgg
       61 gacgacgaaa taaattttgc gaaaatatca tttctgtccc actccctaga ttgatctata
      121 gatactacac ttattaaagt aatatatttt tatgattctc ttagctgcaa tcccatgaat
      181 acatgtaatc atcaaacttc atagcctcaa ggtcagtaga tttcactaag atattggcaa
      241 tacctaaatc accccatata atcgaagaat taccatctct tgtagaatca atttgcagta
      301 agactctatc atacattttc agttcttgat cgttcgttct tggatctatt tggtcaaaat
      361 atggataacc accaagttga ttaaatggct ccttagcagt aaatgtgtta cacacaggtt
      421 ccattaattc atcatatcca ccaaacatcg attttagcgc gaaggatgga ttgactttat
      481 tccacaatgc aagcacgtcc tcatcgtact tataatcagt acatgtaata gtttgtttcg
      541 acaatctaaa ttctatagaa aatgctccac tgaacgggat atcctcatta tcagcatcta
      601 atgacttcaa atgcacttga atattttcga gtgataaatc tgtaattggc tcttttatat
      661 aaacaagcct tgagtttata ttgtttccct ttaaattttc agacatacca tacatttgat
      721 cttctgaact aatccaaaac tgcaatatcc cttcatttac aggaaataat tcttgttgtt
      781 ctggaagatc attcaaatta atctgtgcta acaataccat tggtacatta ttttcatcta
      841 atggtatatc tttaaaagat tttaaaaatg gcattccacc aatcttactc tggcttaaag
      901 tttcttcttg catctctttt aaatccattt caatacatgg tagtactggt aaattaccgt
      961 tttctacaga ttctacgaaa cggttatatg ctttttgctc ctttttagaa agatgactaa
     1021 cgtcttgaat gtttagcata ttaacttccc ccaattttta tttttaaaat agctaaattc
     1081 attattttca tcattcttga ataataaaat gtagcaatac tccaaataat agtgcctaat
     1141 aaattttctt tcatattatt tacttactca gactaaaaat aaactcctca atcttagatt
     1201 aaggagttac acggtgatta ttcaccaatc atttctgaat atttttcagc tgttaataaa
     1261 gcttcaagct gactttcatc actaatttct acttttacca tccatgcttt ttcgtatgga
     1321 gattcattta caaattcggg actatcttct agttcttcgt tgacttcaac tactttacca
     1381 gagattggtg catataattc tgatacagtt ttaactgatt ctacgctacc aaacgtatcc
     1441 ccttcattaa tttcatcatc tgtttctggt aattcaacga aaacaatatc acctaactcg
     1501 ctttgtgcgt attctgtgat tccaattgtt gctacattac cttcaacttt aacccattca
     1561 tgctcttttg aatatttcaa ttcatttggt actgccaaga gaatcccctc ctaattaatc
     1621 tttacatcat taatgatgcc ataaggctag atgctattca actatattta taaaaattaa
     1681 caattgtata gcttatttca ttttacatgt aatgaatcta aatatcgaga tatttagtaa
     1741 acgtttacat ttcacttacg ctaaccaagt ctctttatat tgatcttctt taaatcctaa
     1801 tgttatctta tcgcccatta ctgctagagg acgctttact aacataccat cagatgataa
     1861 caactctaac ttttcatcat ctgataaagt ttgtaattta tttttcaaat caagctcacg
     1921 atatttcgca ccgtgtgtat taaacaattt attaatttct acgcctgtat ttgcaattat
     1981 tgttttaaat tcatttattg taggtgtatg ttgaacgata tcaattggtt cataacttac
     2041 gccatattca tctaaaaact ttgctgcctt tttacaagtt gtacaattct tatattggta
     2101 aaatttaatc at
//
LOCUS       DANBWH010000292         2106 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.301, whole genome shotgun sequence.
ACCESSION   DANBWH010000292 DANBWH010000000
VERSION     DANBWH010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.301"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            461..1609
                     /locus_tag="PPY92_002649"
     CDS             461..1609
                     /locus_tag="PPY92_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001806960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDJ2626586.1"
                     /translation="MRLNKNESELKLCRDLITEFETLSKYNNITNFEVDYKKYVQDVN
                     QHQELSKEIEDKLMQLSQRKLIEQNNLNHYENQLETYNNDLELNEQSIEMEMSRLNLT
                     DDNDINEIIAWRGEQEELEQKRDTYKKRYHEFEMEIARLESLTKDKELLDSDKLKDEY
                     EQKKEKMNTLIDEYSAVHYQCQNNINKTQSIVSHINYLNQELKDQQEIFQLAEIVSGK
                     NNKNLTLENFVLIYYLDQIIAQANLRLATMSDNRYQLIRREAVSHGLSGLEIDVFDLH
                     SNKSRHISSLSGGETFQSSLALALGLSEIVQQQSGGISLESIFIDEGFGTLDQETLET
                     ALDTLLNLKSTGRMVGIISHVSELKNRIPLVLEVKSDQYQSSTRFKRN"
     gene            complement(1680..2042)
                     /gene="mscL"
                     /locus_tag="PPY92_002650"
     CDS             complement(1680..2042)
                     /gene="mscL"
                     /locus_tag="PPY92_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDJ2626587.1"
                     /translation="MLKEFKEFALKGNVLDLAIAVVMGAAFNKIISSLVENIIMPLIG
                     KIFGSVDFAKEWSFWGIKYGLFIQSVIDFIIIAFALFIFVKIANTLMKKEEAEEEAVV
                     EENVVLLTEIRDLLREKK"
BASE COUNT          853 a          290 c          332 g          631 t
ORIGIN      
        1 taatgaagaa acaactataa tgaagaatct cgaaataaaa ttagatcact acgataaatc
       61 aaaattggac ttaaatgata aagaaagctt tataagtgaa attaaatctg ctgtaaagat
      121 tggagatcaa tgtccgatat gtggtaatga aattcaggat ttagggcatc atattgattt
      181 tgacagtatt gctaaacgtc aaaatgaaat taaagaaatt gaagcaaata tccacgcaat
      241 aaaatcgaat attgctgtgc ataattctga aattaaattt gttaatgaaa aaatatcgaa
      301 tattaatatt aaaacgcaaa gtgatttttc actcgaagta ttgaataagc gtctgctaga
      361 aaatgaaaat gcattgaata atcaaagaga tcttaataaa tttatagaac aaatgaaaga
      421 agaaaaagat aatctaacgt tgcaaattca taataaacaa ttgcgtctaa ataaaaatga
      481 atctgagttg aaattatgtc gagatctcat cactgaattt gaaacactct cgaaatataa
      541 taatatcact aattttgagg tggattataa gaagtatgtt caagatgtga atcagcatca
      601 agaactctca aaggagattg aagataagtt aatgcaattg tctcaaagaa agttaattga
      661 gcaaaataat ctaaatcact atgaaaatca actagaaact tacaataatg acttagaatt
      721 gaatgaacaa tctattgaaa tggaaatgtc gaggctgaat ttaactgacg acaatgatat
      781 aaatgaaata atagcctgga ggggcgagca agaggaatta gagcagaaaa gggatactta
      841 taaaaaacgt tatcatgaat ttgaaatgga aatagctagg ttagaatcat taaccaagga
      901 taaagagtta ttggactctg ataaattaaa agatgaatat gagcaaaaaa aagaaaagat
      961 gaatacactg atagacgaat actctgctgt tcattatcaa tgtcaaaata atattaataa
     1021 aacacaatct atagtttcgc atattaatta cttaaatcaa gaattaaagg atcaacaaga
     1081 aatttttcaa ttggctgaaa ttgtcagtgg taagaataac aaaaatctta cattggaaaa
     1141 ctttgtctta atttactatt tagatcaaat tattgcccaa gcaaatctga gattagcaac
     1201 aatgtcagat aatcgatacc aactaattag gcgagaagcg gtttctcatg gtcttagtgg
     1261 cctagaaatt gatgtatttg atttgcattc aaataagtct agacatatta gctcgttatc
     1321 aggtggagaa actttccaat cgtcgcttgc attagcttta gggttaagcg aaattgtaca
     1381 gcagcaatca ggaggtattt cactagaatc aatatttatt gatgaaggat tcggtacatt
     1441 agatcaagaa acgcttgaaa cagcgttaga cactttatta aatcttaaat caactggtag
     1501 aatggttggg attatttcac atgtgagcga attgaaaaat agaatacctt tagttttaga
     1561 agtgaaatca gatcaatatc agagttcaac aagattcaaa agaaattaaa acataaaaaa
     1621 tagagatggt actttggaat gtatagaaca accttagccc atctcttttt atttaaaaat
     1681 tattttttct cacgcaataa atctctgatt tcagttaata acacaacatt ttcttccaca
     1741 actgcttctt cttcggcttc ttctttcttc attaatgtat ttgcaatctt aacaaagatg
     1801 aataaagcaa acgcgatgat aataaagtcg ataacagatt ggataaataa accgtattta
     1861 ataccccaga atgaccattc tttagcaaaa tcaactgatc cgaaaatttt accaattaat
     1921 ggcatgatga tattttctac taatgaagat ataatcttgt tgaaagctgc acccatcaca
     1981 acagcaattg ctaaatctaa gacgttacct tttaaggcga actctttgaa ttcttttaac
     2041 attacactca acctctcttt tttaaatgta acctcaatta tacaacaaat aaaaaaatta
     2101 gaaaat
//
LOCUS       DANBWH010000293         2102 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.302, whole genome shotgun sequence.
ACCESSION   DANBWH010000293 DANBWH010000000
VERSION     DANBWH010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.302"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            390..494
                     /locus_tag="PPY92_002651"
     CDS             390..494
                     /locus_tag="PPY92_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626588.1"
                     /translation="MFKVNYSILSYYPEDNIAVSWQRLREGKTIKNKI"
     gene            622..873
                     /locus_tag="PPY92_002652"
     CDS             622..873
                     /locus_tag="PPY92_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626589.1"
                     /translation="MLTLIKLKEDEQVIIYEYIPEDDISNGKGSVTFNKKDAEVIDFS
                     LSEIENEEYFMLYRNKSFSVVRDFIEKQEFPENYKIAWY"
     gene            949..1152
                     /locus_tag="PPY92_002653"
                     /pseudo
     CDS             949..1152
                     /locus_tag="PPY92_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463904.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            1237..1589
                     /locus_tag="PPY92_002654"
                     /pseudo
     CDS             1237..1589
                     /locus_tag="PPY92_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891995.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1596..1790
                     /locus_tag="PPY92_002655"
     CDS             1596..1790
                     /locus_tag="PPY92_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LSM domain protein"
                     /protein_id="HDJ2626590.1"
                     /translation="MNLQSYIGKLVKLTLTNNKILIGKVIDFDDKVDNFDGYNSIEID
                     TGRITYDISENKIKTIVLQK"
BASE COUNT          858 a          246 c          325 g          673 t
ORIGIN      
        1 aaggagtatg tcgtttcatt tagaatttat tacactaaat tagcatcgaa taagggaaaa
       61 tgagtgttaa gttttaaatg ataaaataga ttggaatgga tcgtcttgaa atgctccctt
      121 caaagttttc attttttcaa tgtctacttc gaaggggcat ttccattata ttgttatagc
      181 tttttatatt tgtataatga acatataagt ttaagaagat gagagtgaag gaaataaaaa
      241 agctcaaatg taccaaattg ttaatcttaa taaatctcta ctttataaag attgaatgga
      301 cattcgagcg ttaatcagtc aggagggaat ttcactccta caatttaata ataatacttg
      361 cttcaacact gtacaaggag tgatttgtta tgttcaaagt gaattattcg attttaagtt
      421 attatccaga agataatatc gcagtaagtt ggcaacgttt aagagaagga aaaacaataa
      481 aaaacaagat ttaatactgc tgcgtcatga ggcgcttgaa cattatttga tgaataagta
      541 taatttcaac tatgattatg catataaaat tgtatcaaaa aatacgatta ttcaattttt
      601 ataaaaaaga aggtggatta aatgcttaca ttaataaaat tgaaagaaga tgaacaggtt
      661 ataatatatg aatatatacc tgaagatgat ataagtaacg gtaaaggttc agtaactttt
      721 aataaaaaag atgcagaggt tatagatttc tcattatctg aaatagaaaa tgaagaatat
      781 tttatgttat atcgtaataa gtctttttct gtagtaagag actttatcga gaagcaagaa
      841 tttcccgaaa attataaaat agcatggtat taaagcatcc tttctacaca gataaagaga
      901 aagcgatgct atttttatat gcttttttca ttattcgttg aaaaagaaga gtcacaaaag
      961 ttaacatatt taaaagaact tggtgaagat gacgaatata aatatgttgc caaaatagat
     1021 agtaaaacat ctaaattatg tcattcactc aacggaaaaa tatttatagt taaagatatg
     1081 ataccaggtg tgaatgcgcc acctatgcat ccttggtgta gaagtaccac agtgccacat
     1141 gttggcaatt ggtgagacaa gttcattaaa gagcgtgaag gtaagtatca agtagaagta
     1201 aaagaagcaa aattacaaga aaaagctaaa aaagaaatgc ttgatatgat aagtaatggt
     1261 aaaataaaga tgcaattgaa taatgaaaaa caacagcgtc atattttaaa cacaaatgaa
     1321 tataataaca agaaaaataa ttcatctttc ttacccagtt atattacaat agattctaat
     1381 gaaattgaaa aggtgactaa aaaagaattt attgattttc cagttttgtt tgatgatgaa
     1441 gggaatttca gaaaaaaaca agttatagat tacaaaaaga taataggtaa atcttatgtg
     1501 aatggtaaat atatgttgaa acgaaattag gtaaagttca ttattctaaa actggttttc
     1561 atgtagttcc gtatattgaa aaggagtgat taaatatgaa tttacagtca tacataggca
     1621 aattagtcaa attaacgctt actaataata aaatattaat tggaaaagtg atagactttg
     1681 atgataaagt tgataatttt gatggttata attcgataga aattgataca ggtagaatta
     1741 catatgatat atcagaaaat aaaattaaaa ctattgtttt acaaaaatag catcctttct
     1801 acacagataa agagaaagcg gtgctatttt ttgcactttt ataacaacta aggatacaag
     1861 acatatacct tcaacaatat cctcatttaa acctatcgat ttcctaagct caattttaaa
     1921 cacaaagttt atttgaaatg atgcacacct ataaaaatct taatcatcga aaagtcacaa
     1981 acatctccat attaaaaact taaatagtgc atttcattta aactaaacat agatattctt
     2041 acaaatatat acttagtaat tcgattgtta atacatcact ttcataaaat aactataacc
     2101 at
//
LOCUS       DANBWH010000294         2089 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.303, whole genome shotgun sequence.
ACCESSION   DANBWH010000294 DANBWH010000000
VERSION     DANBWH010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.303"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            94..2031
                     /gene="isdB"
                     /locus_tag="PPY92_002656"
     CDS             94..2031
                     /gene="isdB"
                     /locus_tag="PPY92_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdB"
                     /protein_id="HDJ2626591.1"
                     /translation="MNKQQKEFKSFYSIRKSSLGVASVAISTLLLLMSNGEAQAAAEE
                     TGGTNTEAQPKTEAVASPTTTSEKAPETKPVANAVSVSNKEVEAPTSETKEAKEVKEV
                     KAPKETKAVKPAAKATNNTYPILNQELREAIKNPAIKDKDHSAPNSRPIDFEMKKENG
                     EQQFYHYASSVKPARVIFTDSKPEIELGLQSGQFWRKFEVYEGDKKLPIKLVSYDTVK
                     DYAYIRFSVSNGTKAVKIVSSTHFNNKEEKYDYTLMEFAQPIYNSADKFKTEEDYKAE
                     KLLAPYKKAKTLERQVYELNKIQDKLPEKLKAEYKKKLEDTKKALDEQVKSAITEFQN
                     VQPTNEKMTDLQDTKYVVYESVENNESMMDTFVKHPIKTGMLNGKKYMVMETTNDDYW
                     KDFMVEGQRVRTISKDAKNNTRTIIFPYVEGKTLYDAIVKVHVKTIDYDGQYHVRIVD
                     KEAFTKANTDKSNKKEQQDNSAKKEATPATPSKPTPSPVEKESQKQDSQKDDNKQLPS
                     VEKENDASSESGKDKTPATKPTKGEVESSSTTPTKVVSTTQNVAKPTTASSKTTKDVV
                     QTSAGSSEAKDSAPLQKANIKNTNDGHTQSQNNKNTQENKAKSLPQTGEESNKDMTLP
                     LMALLALSSIVAFVLPRKRKN"
BASE COUNT          904 a          346 c          326 g          513 t
ORIGIN      
        1 ttgcaattaa ctaccatttc aatgtacaat acaagtaatc aattgataat gattatcagt
       61 tgataatata caattaggag ttgtttctac aacatgaaca aacagcaaaa agaatttaaa
      121 tcattttatt caattagaaa gtcatcacta ggcgttgcat ctgtagcgat tagtacactt
      181 ttattattaa tgtcaaatgg cgaagcacaa gcagcagctg aagaaacagg tggtacaaat
      241 acagaagcac aaccaaaaac tgaagcagtt gcaagtccaa caacaacatc tgaaaaagct
      301 ccagaaacta aaccagtagc taatgctgtc tcagtatcta ataaagaagt tgaggcccct
      361 acttctgaaa caaaagaagc taaagaagtt aaagaagtta aagcccctaa ggaaacaaaa
      421 gcagttaaac cagcagcaaa agccactaac aatacatatc ctattttgaa tcaggaactt
      481 agagaagcga ttaaaaaccc tgcaataaaa gataaagatc atagcgcacc aaactctcgt
      541 ccaattgatt ttgaaatgaa aaaagaaaat ggtgagcaac aattttatca ttatgccagc
      601 tctgttaaac ctgctagagt tattttcact gattcaaaac cagaaattga attaggatta
      661 caatcaggtc aattttggag aaaatttgaa gtttatgaag gtgacaaaaa gttgccaatt
      721 aaattagtat catacgatac tgttaaagat tacgcttaca ttcgcttctc tgtttcaaat
      781 ggaacaaaag ccgttaaaat tgtaagttca actcacttca ataacaaaga agaaaaatac
      841 gattacacat taatggaatt cgcacaacca atttataaca gtgcagataa attcaaaact
      901 gaagaagatt ataaagctga aaaattatta gcgccatata aaaaagcgaa aacactagaa
      961 agacaagttt atgaattaaa taaaattcaa gataaacttc ctgaaaaatt aaaggctgag
     1021 tacaagaaga aattagagga tacaaagaaa gctttagatg agcaagtgaa atcagctatt
     1081 actgaattcc aaaatgtaca accaacaaat gaaaaaatga ctgatttaca agatacaaaa
     1141 tatgttgttt atgaaagtgt tgagaataac gaatctatga tggatacttt tgttaaacac
     1201 cctattaaaa caggtatgct taacggcaaa aaatatatgg tcatggaaac tactaatgac
     1261 gattactgga aagatttcat ggttgaaggt caacgtgtta gaactataag caaagatgct
     1321 aaaaataata ctagaacaat tattttccca tatgttgaag gtaaaactct atatgatgct
     1381 atcgttaaag ttcacgtaaa aacgattgat tatgatggac aataccatgt cagaatcgtt
     1441 gataaagaag catttacaaa agccaatacc gataaatcta acaaaaaaga acaacaagat
     1501 aactcagcta agaaggaagc tactccagct acgcctagca aaccaacacc atcacctgtt
     1561 gaaaaagaat cacaaaaaca agacagccaa aaagatgaca ataaacaatt accaagtgtt
     1621 gaaaaagaaa atgacgcatc tagtgagtca ggtaaagaca aaacgcctgc tacaaaacca
     1681 actaaaggtg aagtagaatc aagtagtaca actccaacta aggtagtatc tacgactcaa
     1741 aatgttgcaa aaccaacaac tgcttcatca aaaacaacaa aagatgttgt tcaaacttca
     1801 gcaggttcta gcgaagcaaa agatagtgct ccattacaaa aagcaaacat taaaaacaca
     1861 aatgatggac acactcaaag ccaaaacaat aaaaatacac aagaaaataa agcaaaatca
     1921 ttaccacaaa ctggtgaaga atcaaataaa gatatgacat taccattaat ggcattacta
     1981 gctttaagta gcatcgttgc attcgtatta cctagaaaac gtaaaaacta ataaatcgtc
     2041 tttatattta attattaaat taacaaattt taattggcgg atgaggtat
//
LOCUS       DANBWH010000295         2073 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.304, whole genome shotgun sequence.
ACCESSION   DANBWH010000295 DANBWH010000000
VERSION     DANBWH010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.304"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            39..1040
                     /gene="argF"
                     /locus_tag="PPY92_002657"
     CDS             39..1040
                     /gene="argF"
                     /locus_tag="PPY92_002657"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDJ2626592.1"
                     /translation="MKNLRNRSFLTLLDFSRQEVEFLLTLSEDLKRAKYIGTEKPMLK
                     NKNIALLFEKDSTRTRCAFEVAAHDQGANVTYLGPTGSQMGKKETTKDTARVLGGMYD
                     GIEYRGFSQRTVETLAEYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKDYADINFTY
                     VGDGRNNVANALMQGAAIMGMNFHLVCPKELNPTDELLNRCKNIAAENGGNILITDDI
                     DQGVKGSDVIYTDVWVSMGEPDEVWKERLELLKPYQVNKEMMDKTGNPNVIFEHCLPS
                     FHNADTKIGQQIFEKYGIREMEVTDEVFESKASVVFQEAENRMHTIKAVMVATLGEF"
     gene            1063..1995
                     /gene="arcC"
                     /locus_tag="PPY92_002658"
     CDS             1063..1995
                     /gene="arcC"
                     /locus_tag="PPY92_002658"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDJ2626593.1"
                     /translation="MAKIVVALGGNALGKSPQEQLELVKNTAKSLVGLITKGHEIVIS
                     HGNGPQVGSINLGLNYAAEHNQGPAFPFAECGAMSQAYIGYQLQESLQNELHSIGMDK
                     QVVTLVTQVEVDENDPAFNNPSKPIGLFYNKEEAEQIQKEKGFIFVEDAGRGYRRVVP
                     SPQPISIIELESIKTLIKNDTLVIAAGGGGIPVIREQHDGFKGIDAVIDKDKTSALLG
                     ANIQCDQLIILTAIDYVYINFNTENQQPLKTTNVDELKRYIDENQFAKGSMLPKIEAA
                     ISFIENNPKGSVLITSLNELDAALEGKVGTVIKK"
BASE COUNT          774 a          283 c          405 g          611 t
ORIGIN      
        1 caatttaggt gtaactaaaa taaaaggagt tgttattaat gaaaaattta cgaaacagaa
       61 gttttttaac tttattagac ttttcacgac aagaggtaga attcttatta acactctccg
      121 aggatttaaa acgtgctaaa tatattggca ctgaaaagcc tatgttaaaa aataaaaata
      181 ttgcactgtt atttgaaaaa gattctacaa gaacgcgatg tgcatttgaa gttgcagcgc
      241 atgatcaagg tgcaaatgta acttatttag gcccaactgg atcacaaatg ggtaaaaaag
      301 aaacaactaa agatactgca cgtgtgcttg gtggaatgta tgatggcatt gaataccgtg
      361 gtttttcaca aagaacagta gaaactttag ctgaatattc aggcgtacca gtgtggaatg
      421 gtttaactga tgaagatcat cctactcaag ttcttgctga tttcttaaca gcaaaagaag
      481 tcttaaaaaa agattatgca gatattaact ttacatatgt tggagatggt cgtaataacg
      541 ttgcaaatgc attaatgcaa ggtgctgcca ttatgggtat gaacttccat ttagtttgtc
      601 caaaagaatt aaatccaaca gatgaattat taaatcgctg taaaaatatt gccgctgaaa
      661 atggtggcaa catattaatc acagatgata ttgaccaagg tgtaaaaggt tcggatgtaa
      721 tttacactga tgtttgggta tcaatgggtg aacctgatga agtatggaaa gaacgacttg
      781 aattattgaa accatatcaa gtaaataaag aaatgatgga taaaactggt aatccaaatg
      841 ttatttttga gcattgctta ccatctttcc ataatgctga tacgaaaatt ggtcaacaaa
      901 tttttgaaaa atatggtatt cgagaaatgg aagttacaga tgaagtattc gaaagtaaag
      961 cttcagttgt attccaagaa gctgagaaca gaatgcatac aatcaaagca gtcatggttg
     1021 ctacattggg tgaattttaa atgatataag gaagtgaata tgatggcgaa aatagtagta
     1081 gcattaggtg gtaatgcttt aggaaaatca cctcaagaac aactcgagct tgttaaaaat
     1141 actgcgaaat cattagtagg attaataaca aaaggacatg agattgttat tagtcatggt
     1201 aatggaccac aggttggaag cattaatttg ggacttaact atgctgcaga acataaccaa
     1261 ggtccggcat ttccatttgc tgaatgtggc gcaatgagtc aagcttacat cggctatcaa
     1321 ttacaagaaa gcttacaaaa tgaattgcat tctattggaa tggataaaca agtggtaaca
     1381 ctagtgacac aagttgaagt tgatgaaaat gatccggcat ttaacaatcc ttcaaaacca
     1441 attgggttat tttacaacaa agaagaagct gaacaaattc aaaaagaaaa aggatttata
     1501 tttgttgaag atgctggaag aggatataga cgcgttgttc cttcaccaca acccatctct
     1561 attattgaat tagagagtat taaaacactt attaaaaatg atacactcgt tattgctgct
     1621 ggtggtggag gtataccagt aattagagag caacatgatg gttttaaagg tattgatgca
     1681 gttatagaca aagataaaac aagtgcgttg ttgggtgcta atattcaatg cgatcaattg
     1741 attattttaa cagcaattga ttatgtatat attaatttta acactgaaaa ccaacagcct
     1801 ttgaaaacaa caaatgttga tgaattaaaa cgatatatag acgaaaatca atttgcaaaa
     1861 ggaagtatgt taccaaaaat tgaagcagcc atatcattta ttgaaaacaa tccaaaagga
     1921 agtgtgctta taacatcatt aaatgaatta gatgctgcct tagagggtaa agtaggtact
     1981 gtgattaaaa agtaattgaa ttgaaacgct tttcaattac tatatgtcaa atgcatgatt
     2041 tttattattt atgtgcaccc cctgaaaata atg
//
LOCUS       DANBWH010000296         2036 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.305, whole genome shotgun sequence.
ACCESSION   DANBWH010000296 DANBWH010000000
VERSION     DANBWH010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.305"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            91..804
                     /locus_tag="PPY92_002659"
     CDS             91..804
                     /locus_tag="PPY92_002659"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDJ2626594.1"
                     /translation="MAKLNVEVFADGADIEEMKAAYKNKQVDGFTTNPSLMAKAGVTD
                     YKAFAEEAVKEIPDASISFEVFADDLETMEKEAAILKQYGENVFVKIPIVNTKGESTI
                     PLIKKLSADNVRLNVTAVYTIEQVKEITEAVTEGVPTYVSVFAGRIADTGVDPLPLMK
                     EAVKVTHSKDGVKLLWASCRELFNVIQADEIGADIITRPADVVKKVNTNLGRDINELS
                     VDTVKGFAKDIQSSGLSIL"
     gene            complement(1244..1879)
                     /gene="sdpA"
                     /locus_tag="PPY92_002660"
     CDS             complement(1244..1879)
                     /gene="sdpA"
                     /locus_tag="PPY92_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpA"
                     /protein_id="HDJ2626595.1"
                     /translation="MQKFKDFFYDDLSVTRGNYFLTLMAAFFITIILFIGIVVSEVHL
                     LYSMLIVLVGLILLRLFKINLFSFKKLTLSQVIYIIGGALLIYGLDNLYLYFHDVPAN
                     ELQLEQEIRNTPFYISIFTVTIIPAIVEEIVFRGMIIRVIFRKHLFLGLLVSSLVFAS
                     LHESDTWIGYLPYLYSGLIFGLIYIKTKRLEVVIFMHFLNNLLALLFIIWG"
BASE COUNT          793 a          270 c          333 g          640 t
ORIGIN      
        1 gaaataaaaa tcatttttta caaatggttg taagaaaaag acatgcagat gttgttaaaa
       61 ttttaataag aatcaaggag gctatattat atggctaaac taaatgtaga agtatttgcg
      121 gacggtgcag atattgaaga aatgaaagca gcttataaaa acaaacaagt ggatggtttt
      181 acaacaaatc ctagtttaat ggcgaaagcg ggcgtaacag attacaaagc ttttgctgaa
      241 gaagctgtga aagaaattcc agatgcttca atttcatttg aagtatttgc agacgattta
      301 gaaactatgg aaaaagaagc agcaatttta aaacaatatg gcgaaaatgt atttgttaaa
      361 attcctattg taaatacaaa aggtgaatca acgattcctt taattaaaaa actttcagct
      421 gacaatgtga gattaaacgt tacggctgtt tacacaattg aacaagttaa agaaataact
      481 gaagcagtaa ctgaaggtgt gccaacatat gtttcagtat ttgcaggacg tattgcagat
      541 acaggcgtag atccattacc attaatgaaa gaggctgtaa aagttacgca tagtaaagac
      601 ggcgttaaat tattatgggc aagttgccgc gaattattca atgtgattca agctgatgaa
      661 attggtgcag atattattac acgccctgca gatgttgtga aaaaagtgaa tacaaactta
      721 ggtcgcgata ttaacgaatt atcagtagat acagttaaag gctttgcgaa agatattcaa
      781 tcttcaggtc tttctattct ataatttata aacatattta aaccctcata ggcattgtgc
      841 ttatgggggt ttttccatca tagttattaa tgaagaagaa gtcaaaattc agctaagtaa
      901 aagcccgagt tataaaaaag tctaaattga ttcaacagga gtagaaaagg aaattagtct
      961 cgcatatttt agaaaatgaa taaaaacaat tactatatgc aactcaagaa ttaaatatta
     1021 tttaagccac atacttatta gagtacaaaa tatacaagtt gtacttatat ctaggtatgt
     1081 gacttttgat tttaagcgta aagcaaaagt ttttatgttt gtattagttc taaattattt
     1141 tgcacttgat gacacgtatt tttgagttta agtggtgact caagttcaat aaaataaata
     1201 tcagatagat aatttgaaaa taatatgaaa ggttatctca aaattatccc catattataa
     1261 agagcagagc taacaagtta tttaagaagt gcatgaatat taccacttct aatcgttttg
     1321 tttttatata aattagacca aaaatcaaac cagaatataa gtaaggtaaa taaccaatcc
     1381 aagtgtcaga ttcgtgtaat gatgcaaaaa ctaaactaga cacaagtaac cctaaaaata
     1441 agtgttttct gaatataacc cttattatca taccgcgaaa aacaatttct tccacaatag
     1501 cggggatgat ggtaacagtg aaaatagaaa tatagaatgg tgtatttcgt atttcttgct
     1561 ctaattgtag ttcatttgcc ggtacgtcat gaaaatataa ataaagatta tctaacccat
     1621 aaattaatag tgcaccgcct ataatataaa taacttgaga caatgttaat tttttaaaag
     1681 agaataaatt gattttgaat agcctcaata gaattaaacc tactaataca attagcatgc
     1741 tataaagtaa atgtacttca ctgacaacta tgccgataaa taaaatgata gtaataaaaa
     1801 atgctgccat taaagttaaa aaataatttc ctcgtgtaac cgataaatca tcgtaaaaaa
     1861 agtctttgaa tttttgcatg ctaaatcctt tcttttcatt ttttaaaatt attttgaatg
     1921 caatatggtt aaggctagat tcattatata tgaaacatat atacgattaa aatttatcat
     1981 tgtacctacc taaaaagcat tagtttgaaa aattagcaga ctgtatttta gcgtca
//
LOCUS       DANBWH010000297         2027 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.306, whole genome shotgun sequence.
ACCESSION   DANBWH010000297 DANBWH010000000
VERSION     DANBWH010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.306"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            163..1830
                     /locus_tag="PPY92_002661"
     CDS             163..1830
                     /locus_tag="PPY92_002661"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDJ2626596.1"
                     /translation="MTHLSDLDIANQSTLQPIKDIAASVGISEDALEPYGHYKAKIDI
                     NKITPRENKGKVVLVTAMSPTPAGEGKSTVTVGLADAFHELNKNVMVALREPALGPTF
                     GIKGGATGGGYAQVLPMEDINLHFNGDFHAITTANNALSAFIDNHIHQGNELGIDQRR
                     IEWKRVLDMNDRALRHVNVGLGGPTNGVPREDGFNITVASEIMAILCLSRSIKDLKDK
                     ISRITIGYTRDRKPVTVADLKVQGALAMILKDAIKPNLVQSIEGTPALVHGGPFANIA
                     HGCNSILATETARDLADIVVTEAGFGSDLGAEKFMDIKAREAGFDLAAVVVVATIRAL
                     KMHGGVAKDNLKEENVEAVKAGIVNLERHVNNIKKFGVEPVVAINAFIHDTDAEVEYV
                     KSWAKENNVRIALTEVWEKGGKGGVDLANEVLEVIDQPNSFKPLYELELPLEQKIEKI
                     VTEIYGGSKVTFSSKAQKQLKQFKENGWDNYPVCMAKTQYSFSDDQTLLGAPSGFEIT
                     IRELEAKTGAGFIVALTGAIMTMPGLPKKPAALNMDVTDDGHAIGLF"
BASE COUNT          724 a          280 c          431 g          592 t
ORIGIN      
        1 acaaaacttt aataagtgaa tttattgcaa aaatgaaagc gctaacccga tttagtcgac
       61 aagtttttaa cagttcgtta ttatatgaat gtaagtaaaa atttcttagc tacaacttac
      121 atattataaa tgcataaatt aaacaaaaag gggcgaaaaa agttgactca tttatcagat
      181 ttagatattg cgaatcaatc aacactacaa ccaattaagg atattgctgc atcagtaggt
      241 atttcagagg atgcattaga accttatggt cattacaaag ctaaaatcga cattaataaa
      301 attacgccaa gagaaaacaa agggaaagtt gttttagtaa ctgcgatgag cccaacacca
      361 gctggtgaag gtaaatcaac ggttacagtt ggtttagctg atgcattcca tgagttaaat
      421 aaaaacgtta tggttgcatt aagagagcct gctttaggac caacatttgg tatcaaaggt
      481 ggtgcgactg gtggtggtta tgcgcaagtc ttacctatgg aagatatcaa cttacatttc
      541 aacggagatt tccatgcgat tacaactgca aataatgcat tgtctgcgtt tatagataat
      601 catattcacc aaggtaatga attaggaatc gatcaaagac gtattgaatg gaaacgtgta
      661 ttagatatga atgatcgtgc acttagacat gtaaacgttg ggttaggtgg acctacaaat
      721 ggtgtaccac gtgaagatgg ctttaatatt acagtagcgt ctgaaattat ggcgatttta
      781 tgtttaagta gaagtattaa agacttaaaa gataaaatta gtcgtattac tattggttac
      841 actagagatc gcaagccagt tacagttgca gatttaaaag tgcaaggtgc acttgcaatg
      901 attttaaaag atgcaattaa accaaactta gtacaatcaa ttgaagggac acctgcatta
      961 gttcatggtg gaccatttgc gaatatcgca cacggttgta actcaatttt agcaactgaa
     1021 acagcacgtg atttagctga tatcgttgta acggaagctg gatttggttc agacttaggc
     1081 gctgaaaaat tcatggacat taaagcgcgt gaagcaggat ttgatctggc agctgtcgtt
     1141 gttgttgcga caattcgtgc gttaaaaatg catggtggtg tagcgaaaga taatttaaaa
     1201 gaagaaaatg tagaagcagt aaaagcagga attgttaatt tagagcgtca tgttaataat
     1261 attaaaaaat tcggtgtaga accggttgtt gcaattaatg catttataca tgataccgat
     1321 gcagaagtag aatatgtaaa atcttgggct aaagaaaata acgtacgaat tgccttaact
     1381 gaagtttggg aaaaaggtgg taaaggtggc gttgacttag caaatgaagt attagaagtc
     1441 attgatcaac ctaattcatt taaaccttta tatgaattag aattaccatt agagcaaaag
     1501 attgaaaaga ttgtgactga aatctatggc ggttcaaaag taacgtttag cagtaaagcg
     1561 caaaaacaat taaaacaatt taaagaaaat ggttgggata attacccagt atgtatggcg
     1621 aaaacacaat attcattctc agatgatcaa acgttgttag gtgcaccatc aggatttgaa
     1681 attacaattc gtgaattaga agcgaaaaca ggtgcaggat ttatcgtagc gttgacaggt
     1741 gcaatcatga ctatgcctgg tttacctaaa aaaccagcag cattaaacat ggatgttact
     1801 gatgatggtc atgcaattgg gttattctaa taaatcatgt caattgttta ataaagataa
     1861 gtaaatagtt taatagaccg gactgttgga gatgcattat ttcagcagtt cggttttttg
     1921 ctgtgctaaa aatagattcc atttggcgaa tctaacgaca atgtttgaag gtggttaatt
     1981 aatgtatatg aagataaaaa gtgggtttga agaataggaa agcgatg
//
LOCUS       DANBWH010000298         2021 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.307, whole genome shotgun sequence.
ACCESSION   DANBWH010000298 DANBWH010000000
VERSION     DANBWH010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.307"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            15..206
                     /locus_tag="PPY92_002662"
     CDS             15..206
                     /locus_tag="PPY92_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaF family protein"
                     /protein_id="HDJ2626597.1"
                     /translation="MKQKKSKNIFWVFSILAVVFLVLFSFAVGASNVPMMILTFILLV
                     ATFGIGFTTKKKYRENDWL"
     gene            complement(360..1187)
                     /locus_tag="PPY92_002663"
     CDS             complement(360..1187)
                     /locus_tag="PPY92_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HDJ2626598.1"
                     /translation="MALYGFAQGLIQEAGIRIKQLMEQNLTIETKSNPNDLVTNVDKA
                     TEDFIFDTILETYPNHQVLGEEGHGHDIDTSKGTVWVVDPIDGTLNFVHQQENFAISI
                     GIYIDGKPYAGFVYDVMADVLYHAKVGEGAYRGSQPLKPLNDSNLRQSIIGINPNWLT
                     KPILGEIFKEIVNDSRSARAYGSAALEIVSVATGNLEAYMTPRLQPWDFAGGLVILYE
                     VNGQASNLLGEPLTISGPNSILVGNRGLHQEISNDYLEPYHDTLIQLHEQRFKRKSK"
     gene            1344..1958
                     /locus_tag="PPY92_002664"
     CDS             1344..1958
                     /locus_tag="PPY92_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YktB family protein"
                     /protein_id="HDJ2626599.1"
                     /translation="MTKYTFKPKDFKAFNVEGLDARMEALNEYIRPQLHELGEYFSDF
                     FTSQTGETFYPHVAKHARRSVNPPKDTWVAFATSKRGYKMLPHFQIGMFEDQLFVMFG
                     IMHEAKDKATRAKVFERKFKAIQQLPDDYRVCLDHMKPDKPFIKDLTDDDLKEAIQRA
                     INVKKGEFFIARAITPQDKRLKSDKAFIAFLEETFDQFLPFYSA"
BASE COUNT          681 a          338 c          314 g          688 t
ORIGIN      
        1 aaggtgggga taggatgaaa caaaaaaaat ctaaaaatat cttttgggta ttttctatat
       61 tagctgttgt tttcttagta ttatttagtt ttgctgttgg tgcatcaaat gtaccaatga
      121 tgattttaac atttatatta ctcgttgcaa cctttggaat tggatttact acaaagaaaa
      181 aatatcgaga aaacgattgg ctataagtta tatcgacaat aacacgcgtt gatttaagac
      241 cacacatgtt cattatgaac atttaaatgg ttttatattg gcgcgtgttt ttattattta
      301 aggtataaaa gcgtaatatg ttacctaatt gttaggacta catattacgc ttttggtaat
      361 tattttgatt ttcttttaaa tcgttgttca tgtaattgta ttaacgtatc atggtagggc
      421 tctaaataat cattgctaat ttcttgatgg agacctcgat ttccaactaa gattgaattt
      481 ggaccactaa ttgttaatgg ttctcctagt aaatttgaag cttgtccatt tacttcatat
      541 aaaataacca atccgccagc aaaatcccat ggttgaagtc ttggcgtcat atatgcttct
      601 aaattacctg tagcaactga aacgatttca agcgctgcac taccatatgc ccttgcactt
      661 ctagaatcat taacaatttc tttaaagatt tctcctaaaa ttggtttagt taaccagttc
      721 ggattgatcc caataatgct ttgtcttaga ttagaatcat tcaatggttt caagggttgg
      781 ctacctcgat atgcgccttc ccctacttta gcatgatata agacgtcagc cataacatca
      841 tatacaaaac ctgcataagg tttaccatcg atataaatac caattgaaat tgcgaaattt
      901 tcttgttgat gaacaaaatt caatgtaccg tctattgggt caacaaccca taccgtacct
      961 ttggaagtat cgatgtcatg accatgccct tcttcaccta atacttgatg attgggatat
     1021 gtttctaaaa ttgtatcaaa aatgaaatct tctgttgctt tatctacatt tgtaacaagg
     1081 tcattcggat ttgactttgt ttcaattgtt aaattttgct ccatcaattg tttaattcta
     1141 attcctgctt cttgaataag tccttgggca aatccataaa gtgccataac atcatctcct
     1201 aacatcttta ttatacatca acattttata aaaataactt cttatgataa aaatgaaaat
     1261 actaatttta atgagagcgt cttaagccgc aattgataaa acatatgcta caatattttt
     1321 aaacactata caggaggtac tctatgacaa aatatacatt taaacctaaa gatttcaaag
     1381 cgttcaacgt agaaggctta gacgcacgaa tggaagcttt aaacgaatac atacgaccac
     1441 aactccatga attaggagaa tattttagtg acttctttac aagtcaaact ggtgaaacat
     1501 tttatcctca cgtagcaaaa catgctagaa gaagtgtgaa tcctcctaaa gatacatggg
     1561 ttgcttttgc tacaagcaaa agaggctata aaatgttacc tcatttccaa attggtatgt
     1621 ttgaagatca actgtttgtt atgtttggaa tcatgcatga agcaaaagat aaagcaacac
     1681 gtgcaaaagt ttttgaaaga aaatttaaag ctattcaaca attacctgat gattatcgtg
     1741 tttgcttaga tcatatgaaa cctgataaac catttattaa agatttaacg gatgatgatt
     1801 taaaagaagc gatacaaaga gccatcaatg tgaaaaaagg tgaattcttt atagcgcgtg
     1861 caatcacacc acaagataaa agattaaaaa gtgacaaagc atttattgca tttttagaag
     1921 aaaccttcga tcagttctta ccattttatt ctgcataaat aactttgttt aaataataga
     1981 gcacgtaatc acatccatga tttcgtgctc ttttttctta a
//
LOCUS       DANBWH010000299         2012 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.308, whole genome shotgun sequence.
ACCESSION   DANBWH010000299 DANBWH010000000
VERSION     DANBWH010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.308"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(116..358)
                     /locus_tag="PPY92_002665"
     CDS             complement(116..358)
                     /locus_tag="PPY92_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HDJ2626600.1"
                     /translation="MPTEDTTMFDQVAEVIERLRPFLLRDGGDCSLIDVEDGIVKLQL
                     HGACGTCPSSTITLKAGIERALHEEVPGVIEVEQVF"
     gene            459..782
                     /locus_tag="PPY92_002666"
     CDS             459..782
                     /locus_tag="PPY92_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzD family protein"
                     /protein_id="HDJ2626601.1"
                     /translation="MEHVSVVVYGADVICASCVNAPTSKDIYDWLQPLLKRKYPNISF
                     KYTYIDITKDNDNLTDHDLQFIERIEQDELFYPLITMNDEYVADGYIQTKQITRFIDQ
                     KLVNE"
     gene            complement(841..1905)
                     /locus_tag="PPY92_002667"
     CDS             complement(841..1905)
                     /locus_tag="PPY92_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDJ2626602.1"
                     /translation="MKNLVLLGGGYGNMRIMSRILTTSLPQDYTVTLVDRMPFHGLKP
                     EFYALAAGTKSDKDVRMKFPNHPQVNTVYGEINDIDLDAQIVSVGNSKIDYDELIIGL
                     GCEDKYHNVPGAEEYTHSIQTLSKARDTFHSISELPEGAKVGIVGAGLSGIELASELR
                     ESRSDLEIYLYDRGPRILRNFPEKLSKYVAKWFAKNNVTVVPNSNINKVEPGKIYNCD
                     EPKDIDLVVWTAGIQPVEVVRNLPIDINSNGRVIVNQYHQVPTYRNVYVVGDCADLPH
                     APSAQLAEVQGDQIADVLKKQWLNEPLPDKMPELKVQGIVGSLGDKQGFAYIMDRTVT
                     GRLASILKSGVLWLYKYHNG"
BASE COUNT          667 a          365 c          322 g          658 t
ORIGIN      
        1 cccatcagtc caatttgaca gcgaaaaaag acaggtaata actgattata aataattcag
       61 tattcctgtc tttgttgtta ttcataatat gttctgttaa cttaatatct ttatattaga
      121 atacttgttc tacttctatt acaccaggca cttcttcgtg taatgcacgc tcaataccag
      181 ctttaagagt gattgtagaa cttgggcatg taccacatgc accatgtaat tgtaatttaa
      241 caataccgtc ttcaacgtca atcaatgagc agtcgccacc atcacgtaat aaaaatggac
      301 gaagacgttc aataacttct gctacttgat caaacatcgt tgtatcttca gtaggcatca
      361 ggcaagtctc ctttcgagag atgaatttga taatctttat tagtatatta taatagatag
      421 tgtaataaaa atctatcaaa ccaatggggg ataaatatat ggagcacgtg agtgtggtag
      481 tatatggggc agatgttata tgtgcaagtt gcgttaatgc gccaacatcg aaagatattt
      541 atgactggct acagccgcta ttaaaaagaa aatacccaaa tatatcattt aaatatacgt
      601 atatagatat tacaaaagat aatgacaact taacagatca tgatttacaa tttattgaaa
      661 gaatagaaca agatgaacta ttttatccat taattacaat gaacgatgaa tatgtagcag
      721 atggttatat acaaacaaag caaatcactc gatttataga ccaaaagctt gtgaatgaat
      781 aatatacaca taacaaaaag caatgtcagt aatagtgtga actgacattg ctttttaatt
      841 ttaaccatta tgatatttat ataaccaaag tacgccggat tttaaaatag aagcgagtcg
      901 tcctgttaca gtgcggtcca tgatatatgc aaaaccttgt ttatctccta atgacccaac
      961 gataccttgt acctttagtt ccggcatttt gtcaggtaat ggttcattta gccattgctt
     1021 tttaagcaca tcggcaattt gatcaccttg aacttcggct aactgagcac ttggcgcatg
     1081 tggtaaatca gcacaatcac caactacata gacgttacga tatgttggta cttgatgata
     1141 ctggttaact atcacgcgtc cattactatt tatatcaatc ggcaagttac gaacaacttc
     1201 aacaggttga attcctgctg tccatacaac taaatcaata tctttaggtt catcacagtt
     1261 atatatttta ccaggttcaa ctttattaat atttgaattt ggaacaacgg taacattatt
     1321 tttggcgaac catttcgcaa catacttact taatttttct ggaaaatttc ttaaaattcg
     1381 cggcccacgg tcataaagat atatttccaa gtctgatcta ctttctctta attcgctggc
     1441 aagttctatg ccacttaatc cagcaccaac gataccaact ttagcacctt ctggtagttc
     1501 actaatacta tggaaagtat cccgagcctt tgagagtgtt tgaatactat gtgtatattc
     1561 ttcggctcct ggaacgttat gatacttatc ttcacatcct aaaccaatga ttagctcatc
     1621 ataatcaatt ttagaattac cgactgagac aatttgagca tctaaatcta tgtcgttaat
     1681 ttcaccataa actgtattca cttgtggatg attagggaat ttcatgcgaa catctttatc
     1741 tgatttcgtg cccgcagcta aagcatagaa ttctggtttc aatccatgaa atggcatacg
     1801 atcaactaat gtgactgtat aatcttgtgg taaagaagta gttaaaatgc gtgacatgat
     1861 acgcatatta ccatatccgc cgcctaacaa aactaagttt ttcataatga agtacccctt
     1921 ttatatgtta atatttactt taattttgaa aagatcaaag tgcatagata tatttgttaa
     1981 tgaacagtta taaaatgcaa gtgtctttga tt
//
LOCUS       DANBWH010000300         1998 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.310, whole genome shotgun sequence.
ACCESSION   DANBWH010000300 DANBWH010000000
VERSION     DANBWH010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.310"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..1598)
                     /locus_tag="PPY92_002668"
     CDS             complement(<1..1598)
                     /locus_tag="PPY92_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ligase"
                     /protein_id="HDJ2626603.1"
                     /translation="MLISAGLKDYYPLQNRFNNNIRSAVYLLLCKMIRQPNFAVLEVS
                     LNALNAVGNSSYLIKPNIAIVTGIGAAHMSTFKDILNIVEVKASIFDGLTPEGVAIIN
                     KDTLHSDILIERAKQNTSNVITYSTHDSSATICPKSIQYSKGYTVITIDFNGQKYTYR
                     INSISDGMVENSLATFATLSHLDIPLERALENLSTFKPFEKVLNLKEVETPNYKVNLI
                     DDTHNASLPAMINAIKAFNTQTKFFKGNKIIAIGQISDLGKHSKSLHLQLVDVLENSN
                     ADYILCMDDALKSVVIGVKSKNITWYSNRHLLEKDLLYLNKPDSLTLLKSSAGGTEFP
                     KLAKELPEKLNKYNINNSNTSLFDGQSLNGRSYMIIDENYNVIESHNREHSGTIEGLG
                     PIFNYLKAIDDNVSEDTIFIANWATNNKLYYEGKETTTYELMKAMLNSPMYTPSYELS
                     KYLFENGPKRDEYINSKIEHLSLSNSVAINLTGRHTMRERQNFTVDDLFKILKAYKNT
                     LFKFTNEIIIGRKYNSGIIKDKDK"
     gene            complement(1592..1990)
                     /locus_tag="PPY92_002669"
     CDS             complement(1592..1990)
                     /locus_tag="PPY92_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626604.1"
                     /translation="MSLTIKELHNILGGNLLDAEGKEDNVINNFENWFPFIHNKSTAY
                     ISPNKHTWRRFGRDANAKEGNEYIKKDKEDLGLIITETYVPDMKFKTPQLVINDSANT
                     LTKLAEEMRLQYKHPVVAITGSMGKVQLEC"
BASE COUNT          627 a          297 c          278 g          796 t
ORIGIN      
        1 aatttatctt tatcttttat aataccgcta ttatatttcc tgccaataat tatttcgtta
       61 gtaaatttaa atagggtatt tttatatgct tttaaaattt taaataagtc gtcaacagtg
      121 aagttttgtc gttctctcat agtatgacgt ccagttaaat ttattgctac agaatttgaa
      181 agacttaaat gttctatttt tgaattgata tattcatcgc gctttggacc gttttcaaat
      241 aaatacttag ataattcata agaaggtgta tacataggtg aattaagcat ggctttcatc
      301 aattcgtaag ttgtcgtttc ttttccttca taataaagtt tgttatttgt tgcccagtta
      361 gcaataaaaa tagtatcttc tgatacatta tcatctatag cttttaaata attaaagata
      421 gggcctaagc cttcaattgt acctgaatgt tctctgttat gagattcaat gacattataa
      481 ttttcatcaa taatcatata actgcgaccg ttcaaggatt gaccatcaaa taaagaagtg
      541 ttcgaattat taatattata tttatttaat ttctcaggta attcttttgc taattttgga
      601 aattctgtac cacctgctga tgattttaat agagttaatg aatctggttt atttaggtac
      661 aataaatctt tttctaatag atgtctatta gaataccatg ttatattttt gctttttact
      721 cctataacga cactctttaa agcatcatcc atacatagaa tataatctgc attactattt
      781 tctagaacat caactaattg caaatgcaat gatttagagt gcttacctaa gtcactaatt
      841 tgcccaatcg caataatttt attaccttta aaaaattttg tttgagtatt aaatgctttt
      901 atagcattaa tcattgctgg taatgaagcg ttgtgtgtat catctattaa gttaacttta
      961 tagttaggtg tttcaacttc ttttaggttt aaaacttttt caaatggttt aaatgtactt
     1021 aagttctcta aggctctttc tagtggtata tctaaatgcg ataatgtagc aaatgtggct
     1081 agagagtttt caaccatacc gtcacttatt gaattaattc tataagtata tttttgtccg
     1141 ttaaaatcaa ttgttataac tgtatagcct ttactatatt gaatagattt aggacaaatt
     1201 gttgcgctag agtcatgtgt actataagta ataacattac ttgtattttg tttggcgcgt
     1261 tcgattaata tatctgaatg taatgtatct ttattaatta ttgctactcc ctcaggagtt
     1321 aaaccatcaa aaatacttgc tttcacttct acaatattta gaatatcttt aaatgtagac
     1381 atatgagcag cacctatacc agtaactata gcaatatttg gttttattaa atatgatgaa
     1441 ttaccaactg catttaatgc gtttaatgat acttctaaaa cagcaaaatt tggttgtcta
     1501 atcattttgc ataataataa atatactgcg gatcttatgt tattattaaa tctattttga
     1561 agaggataat aatctttaag accagcactt atcagcattc tagttgaact tttcccattg
     1621 atcctgttat agcaacaaca gggtgtttat actgtagtct catttcttca gctaatttag
     1681 ttagtgtgtt tgcagaatca ttgataacta attgaggagt tttaaacttc atgtcaggaa
     1741 cataggtttc agtaataatt aatcctaaat cttccttatc cttctttata tattcattac
     1801 cttcttttgc gtttgcatct ctaccaaaac gtctccatgt atgcttattg ggcgatatgt
     1861 aggctgtgct tttattatga ataaagggga accaattctc aaaattattt ataacattgt
     1921 cctctttacc ttcagcatca aggagattac ctcctaaaat attatgaagt tctttaattg
     1981 ttaatgacat tttgtact
//
LOCUS       DANBWH010000301         1987 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.311, whole genome shotgun sequence.
ACCESSION   DANBWH010000301 DANBWH010000000
VERSION     DANBWH010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.311"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..775
                     /locus_tag="PPY92_002670"
     CDS             <1..775
                     /locus_tag="PPY92_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDJ2626605.1"
                     /translation="KKDKKGVDICFEDATRTSREKLKEYMEIISKYQVRTVTFADTVG
                     CSTPLEYGDIFNYFVKKYSNIIFSAHCHNDLGLATANTLAAILNGAKQIETTFLGIGE
                     RAGNAPIEEIITILTKKQIESTEFTLPDVYKTSINISKILDFQISENKPIIGENIFKH
                     ESGIHQDGTKKNINMYQYLVPSDLGLKNSQVVQFPISNISSKKILHNKFKSIVNTEEI
                     DENISFYKLVNKFSPEVAPEDTVDLLQIIKRRSKDGNFK"
     gene            759..1400
                     /locus_tag="PPY92_002671"
     CDS             759..1400
                     /locus_tag="PPY92_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HDJ2626606.1"
                     /translation="MEILNDSVKSFEKLYLQLQDGFPVIVPTDTNYNLCSLPNNDLCI
                     DKIFEYKKRSKDKPLSLFIDKPEDWKLYGDNQNTEIVDKLVEIFWPGPLNIILKNKTS
                     YNYMLNNSDSIAIGCVQNKTMRRFISYINSPIAITSANISGTADDILITENEAIKHMG
                     ENVRYMLRSQNKTNYKTSSTIIKVTDNKIELLREGDIKFEEIKERLGTGIIYE"
     gene            1393..>1987
                     /locus_tag="PPY92_002672"
     CDS             1393..>1987
                     /locus_tag="PPY92_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626607.1"
                     /translation="MNKLILGIYLYRIFSRAYFYLPFLLIYFLIQGYSIIQLEILMAS
                     YGIAAFLFSLYKEKCFKICNLKDSNKLVVSEIFKIIGLLLLLYQNQYLILVVAQILLG
                     LSYSMMAGVDTAIIKRNITNEKYVQNKSNSYMFLSLLISGIIGSYLYGINIKWPIIMT
                     GIFSILTIIIIRCTLVENRELNLIGETKGKIKKFLPEE"
BASE COUNT          800 a          217 c          313 g          657 t
ORIGIN      
        1 aaagaaagat aaaaaaggag tagatatttg ttttgaagat gcaacaagga cttctagaga
       61 aaaattgaaa gaatacatgg aaattatttc aaaatatcaa gttagaacag ttacatttgc
      121 ggacactgta ggatgttcga caccattaga atacggagat atttttaatt actttgtaaa
      181 aaaatattct aacataattt tttctgctca ttgtcataac gatctagggt tggctactgc
      241 aaatacatta gctgcaattt taaatggtgc aaagcaaata gaaactacat ttttgggaat
      301 tggtgagaga gcgggtaatg ctcctattga ggaaataatt actattttga caaaaaaaca
      361 aatagaaagt acggaattca ctttacccga cgtatataaa actagtatta atatttctaa
      421 aattctcgat tttcaaatat cagaaaacaa acctataatt ggtgaaaata tatttaaaca
      481 tgaatcagga attcatcaag atggtactaa aaaaaatata aatatgtatc aatatttagt
      541 tcctagtgat ttaggattga aaaattcaca agttgttcag tttccaataa gtaatatttc
      601 tagtaagaaa atcttgcaca ataaatttaa atcaatagtt aacactgaag aaattgatga
      661 aaatatttct ttctataaac ttgtgaacaa gttttcacct gaagtagcac ctgaagatac
      721 agtggattta cttcagataa taaaaaggag gagtaaagat ggaaatttta aatgattctg
      781 taaaaagttt tgaaaaactt tatttacaat tacaagatgg ttttccagtg attgttccta
      841 ctgatactaa ttataattta tgtagtttac caaacaatga tctttgtatc gataaaatat
      901 ttgaatataa aaagagatcg aaagataagc cattatcatt atttattgat aagccagagg
      961 attggaaatt gtatggagat aatcaaaata cggaaatagt tgataaacta gttgaaatat
     1021 tttggcctgg accattaaat attattttaa aaaataaaac aagctataat tatatgctca
     1081 ataattcaga tagtatagct ataggatgtg tgcaaaataa aacgatgaga aggtttattt
     1141 catatattaa ttcacctata gcaattactt cagcaaatat ctctggaact gccgatgata
     1201 ttttaataac tgaaaatgaa gcaattaagc acatgggcga aaatgtaaga tatatgctaa
     1261 gaagtcaaaa taaaactaac tataaaacat ctagtacgat tattaaagtg acagataata
     1321 aaattgaatt attaagagaa ggagatataa agtttgaaga aataaaagaa agactaggta
     1381 caggtattat ttatgaataa gttaatactt gggatttatt tgtaccgaat tttttcacga
     1441 gcatactttt atttaccgtt tttattaatt tactttttga ttcaaggtta ttccataata
     1501 caattagaaa tattaatggc gtcttatggc attgcagcat ttttattctc tctatacaaa
     1561 gagaagtgtt ttaaaatttg taacttaaaa gattctaata aattagttgt tagtgaaata
     1621 ttcaaaatca tcggtttatt gttgttatta tatcaaaatc aatatttaat tttagtagtg
     1681 gcacaaatat tattagggtt aagttactca atgatggcgg gtgttgatac cgcaataatt
     1741 aaaagaaata taacaaatga gaaatacgta caaaataagt caaatagcta tatgttccta
     1801 tcattattaa tttcagggat tataggtagt tatctttatg gaataaatat taaatggcct
     1861 ataataatga ctggtatatt ttcaattcta acaattataa ttattcgatg cacattagtt
     1921 gaaaataggg aattaaattt aataggagaa acaaagggaa agataaagaa atttctaccg
     1981 gaagaga
//
LOCUS       DANBWH010000302         1968 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.312, whole genome shotgun sequence.
ACCESSION   DANBWH010000302 DANBWH010000000
VERSION     DANBWH010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.312"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(285..1931)
                     /locus_tag="PPY92_002673"
     CDS             complement(285..1931)
                     /locus_tag="PPY92_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDJ2626608.1"
                     /translation="MNSSSPENPNGKKYSPVFIYSAIVVAIVVLLGAFLPEQFNYVTN
                     NIKMWITEKLGWYYLILTTIIVFFCIFLIFSPIGKLKLGKPNDKPEFNTISWFAMLFS
                     AGMGIGLVFYGAAEPMAHFATPPTADPKTTEAYTEALRSTFFHWGFHAWAVYGVVALA
                     LAYSQFRKGEPGLLSRTLRPLLGDKVEGPIGIFIDVLSVFATIVGVAVSLGMGALQIN
                     GGLHYLFNVPNNTFVQAIIIIVVTILFIASAWSGLSKGIQYLSNLNIGLGTILMVAAL
                     IVGPTVLILNMLTSSTGSLLNTFLFNSFDTAALNPQKREWMSSWTLYYWGWWLSWSPF
                     VGVFIARVSKGRSIREFISGVLLVPAIVSFVWFSVFGVLGIETGKKHKEIFDMTPETQ
                     LFGVFNHVPFGIVLSLIALLLIASFFITSADSATFVLGMQTTFGSLNPSSMVKVVWGI
                     SQALIAFVLLLAGGGNGAEALNAIQSAAIISAFPFSFVVILMMVSFYKDANQERKFLG
                     LTLTPNKHRLQEYIKSQQEDYESDILEKRQSRRNIEKKDN"
BASE COUNT          704 a          382 c          288 g          594 t
ORIGIN      
        1 attgatctat gtcccagtct gagttaattt tctataaaag tatagttaat ttttttatac
       61 cgtcgtactt cactttagct ttgtcaaacc tctttctatt aagttgtcag aaataaacct
      121 atcttaaaat ataaaaaaat cgagaattcg tagtttaata acgaaattct cgttcttatc
      181 cttttgaata tactcaattt tccacaaaaa caaacaagta gtatatctgt tctagctact
      241 agaatgacat actacttgtt atttaaatac ttaactaaat tttattagtt atcttttttc
      301 tctatatttc tacgtgactg acgcttttca agaatgtcag attcataatc ttcttgttga
      361 ctcttgatat attcttgtaa gcgatgttta ttcggagtca atgttaaacc taggaattta
      421 cgttcctggt tcgcatcctt gtagaaactt accatcatga gtatgacgac aaaggagaat
      481 gggaatgcac ttataattgc agcactttga atcgcattta aagcttcagc gccattaccg
      541 ccaccagcta ataaaagtac aaatgctatt aaggcctgtg aaattcccca aacaactttt
      601 accatactag atggatttaa tgaaccaaat gttgtttgca ttcctaatac aaatgttgct
      661 gagtcagcag atgtaataaa gaatgatgca attaataata atgcaatcaa cgataaaact
      721 atgccaaatg gcacatgatt aaacactcca aatagctgtg tttcaggagt catatcaaaa
      781 atttctttgt gtttcttacc tgtctcgatg cctaatacac caaagacact aaaccaaaca
      841 aaactaacaa ttgctggaac tagcaagaca ccagaaatga actctctaat tgaacgtcct
      901 tttgaaactc gtgcaataaa cactccaacg aatgggctcc aacttaacca ccaaccccag
      961 taataaagtg tccatgaaga catccattca cgtttttgag gatttaaagc agctgtatca
     1021 aaactattaa acaagaatgt gtttagtaaa ctacccgtag agctagttaa catatttaaa
     1081 ataagaacag ttggtccaac aattaaagca gctaccatta aaatagtacc taaaccaatg
     1141 ttcaagttac ttaagtattg aataccttta cttaatccag accatgcact tgctataaat
     1201 aagatagtaa caacaatgat gataatcgct tgtacaaacg tattgtttgg aacattgaac
     1261 aagtaatgta aaccaccatt aatttgtaga gcacccatac ctaacgaaac ggctacccca
     1321 acgattgtcg caaatacaga taaaacgtca ataaaaatcc caataggacc ttctacttta
     1381 tcacctaaaa gaggacgtaa agttctagat aataaacctg gttcaccttt acggaattgc
     1441 gaatatgcca acgctaacgc aacaacacca taaacagccc aagcatggaa tccccaatgg
     1501 aaaaatgttg aacgtagagc ttcagtataa gcttcagtag ttttgggatc tgctgtaggt
     1561 ggcgtagcaa agtgcgccat cggttcagct gcaccataaa acaccaaacc tatccccata
     1621 ccagcactaa acaacatagc aaaccatgaa attgtattaa actcaggttt gtcatttggt
     1681 ttacctagtt taagttttcc aataggacta aaaataagga atatacagaa gaacacgata
     1741 atcgtagtaa gaataagata ataccaacct aacttttctg taatccacat tttaatatta
     1801 ttggtaacat agttgaattg ttcaggtaaa aatgcaccaa gtaatacgac tatagcaaca
     1861 acaattgcac tatagatgaa gactggtgaa tacttctttc catttggatt ctctggtgaa
     1921 gaagaattca taattaatta ctcccttcaa ttctatattt aattttat
//
LOCUS       DANBWH010000303         1964 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.313, whole genome shotgun sequence.
ACCESSION   DANBWH010000303 DANBWH010000000
VERSION     DANBWH010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.313"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..918
                     /locus_tag="PPY92_002674"
     CDS             <1..918
                     /locus_tag="PPY92_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDJ2626609.1"
                     /translation="IISVYYIFWLLAKRKYILVTFWTIIILTLLITFPTNSHHKITML
                     NVGQGDSILYEGGKNQNVLIDTGGKVIDDTKQPSYSISKYHILPTLNERGINELEYLI
                     LTHPHNDHIGEVEYIISHIKIKHIVIYNKGYSSNTLMLLSKLSHKYNIKLMDVRQVSS
                     FKLGDSSFLFFDSFIPNSRDKNEYSIITMITYQNKKVLLMGDASKNNESLLLKKYNLP
                     EIDILKVGHHGSKTSSSKEFIEMIKPKISLISSGKNNMYHLPNIEVVKRLQRIRSRIY
                     NSQQNGQVTIDLDDNLKVDSNSYGNASGL"
     gene            975..1949
                     /gene="holA"
                     /locus_tag="PPY92_002675"
     CDS             975..1949
                     /gene="holA"
                     /locus_tag="PPY92_002675"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDJ2626610.1"
                     /translation="MSDNIVAIYGDVPELVEKQSAEIISQFLKSDRDDFNFVKYNLYE
                     TEIAPIVEETLTLPFFSDKKAILVKNAYIFTGEKAPKDMAHNVDQLIEFIEKYDGENL
                     IVFEIYQNKLDERKKLTKTLKKHARLKKIEQMSEEEIKKWIQSKLNENFKDIKRDALD
                     LFIELTGINFNIVSQEIEKLILFLGDRPTINKQDVNQIINRSLEQNVFLLTEYIQKRK
                     KEQAIHLVKDLITMKEEPIKLLALITSNYRLFYQCKILSQKGYSGQQIAKTIGVHPYR
                     VKLALGQVRHYQLDELLNIVDACAETDYKLKSSYMDKQLILELFILSL"
BASE COUNT          803 a          214 c          327 g          620 t
ORIGIN      
        1 ataatttctg tttattacat attttggtta ttggctaaac gtaaatatat attggttacg
       61 ttttggacta taattattct gacattatta ataacgtttc caacaaattc acatcacaaa
      121 attacaatgt taaatgtggg gcagggagac agtattttat atgaaggcgg taagaaccaa
      181 aatgtcttga ttgatacagg tgggaaagtg attgatgata ctaaacaacc tagttattca
      241 atttctaaat atcatatttt accaacgcta aatgaaagag ggataaatga attagagtat
      301 ctaattttaa cacatccaca caatgaccat attggtgaag tggaatatat tattagtcat
      361 attaaaatta aacatatagt gatatacaat aagggatata gtagtaatac attgatgtta
      421 ttatcgaaat taagccataa gtacaacatt aaacttatgg atgtaagaca agttagtagt
      481 tttaaacttg gagatagtag ttttctattt tttgatagtt ttattccaaa tagccgagat
      541 aaaaatgagt attcgattat tactatgatt acatatcaaa ataaaaaagt tttattaatg
      601 ggcgatgcta gtaaaaataa tgaatcttta ctactaaaaa aatataactt gccggagatt
      661 gatattttaa aagtaggaca tcatgggagc aagacaagta gttctaaaga atttatagag
      721 atgattaagc ctaaaataag tttgatttct tctgggaaga acaatatgta tcatcttcct
      781 aatatagaag ttgttaaacg attgcaaagg attcgcagtc gcatttacaa tagtcaacaa
      841 aacggtcaag ttacaattga cttagatgat aatttaaaag ttgattcaaa ctcttatgga
      901 aatgcaagtg gtttatagat acagaaacat gatataatct attgatgtgt caaacgaatg
      961 aaagggtgta atgaatgagc gacaatattg tagctattta tggagatgtg cctgaattgg
     1021 ttgaaaaaca aagtgcagaa atcatatcac aatttttgaa aagtgataga gatgacttta
     1081 actttgtgaa atataattta tacgaaacag agattgcacc aattgttgaa gaaacattaa
     1141 cattgccttt cttttcagat aaaaaagcaa ttttggttaa aaatgcatat atatttacag
     1201 gtgaaaaagc gccaaaagat atggctcata atgtagatca attaatagaa tttattgaaa
     1261 aatatgatgg cgaaaatttg attgtctttg agatatatca aaataaactt gatgaaagaa
     1321 aaaagttaac taaaactcta aaaaagcatg caaggcttaa aaaaatagag caaatgtctg
     1381 aagaagaaat aaaaaaatgg attcaaagta aattaaatga gaatttcaaa gatatcaaaa
     1441 gagatgcatt agatttattt attgagttga caggtattaa ctttaatatt gtctcacaag
     1501 agatagaaaa gttgatttta tttttaggcg atagaccaac aattaataag caggatgtta
     1561 accaaattat taatagaagt ttagagcaaa atgtattttt actgactgaa tacattcaga
     1621 aaagaaagaa agaacaagca attcatttag taaaagattt aataactatg aaagaagaac
     1681 caattaaatt acttgcacta attacaagta attaccgatt attttatcaa tgtaagattc
     1741 tgagtcaaaa aggatatagt ggacagcaaa ttgctaaaac aataggagtg catccataca
     1801 gagtaaaatt agcgttagga caagtaagac attatcaact tgatgaatta ttgaatattg
     1861 tagatgcttg tgcggaaact gattataaac ttaaatcatc atatatggat aaacagttaa
     1921 tactggaatt attcattcta tctttataaa aaataaaagt tcaa
//
LOCUS       DANBWH010000304         1960 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.314, whole genome shotgun sequence.
ACCESSION   DANBWH010000304 DANBWH010000000
VERSION     DANBWH010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.314"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(156..1862)
                     /gene="acsA"
                     /locus_tag="PPY92_002676"
     CDS             complement(156..1862)
                     /gene="acsA"
                     /locus_tag="PPY92_002676"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HDJ2626611.1"
                     /translation="MKVEVYKGAQGKHNLKDYEETYNTFDWKDVEQAFSWSETGKMNM
                     AYECIDRHVDQGLGDKIALNYKDEYRKESYTYKDMQRLSNKAANVLSEHAEVDKGDRV
                     FIFMSRTPELYFALLGVLKIGAIVGPLFEAFMEKAVADRLENSEAKVLITNKALLPRV
                     PVDKLPNLKKIVVVDEDVEDNYIDFISLMETASDEFDIEWLKSDDGLILHYTSGSTGQ
                     PKGVLHVQQAMLVHYISGKYVLDLQEDDVYWCTADPGWVTGTSYGIFAPWLNGATNCI
                     AGGRFSPEQWYSMIEDFKVTIWYTAPTALRMLMSAGDDIVEKYDLSSLRSILSVGEPL
                     NPEVIKWAKKVYGLTVLDTWWMTETGGHMIVNYPTMDVKLGSMGKPLPGIQAAIIDDA
                     GNELPPNRMGNLAIKKGWPSMMYRIWKNPEKYKSYFIGDWYVSGDSAYKDEDGYFWFQ
                     GRVDDVIMTAGERVGPFEVESKLVEHEAVAEAGIIGKPDPVRGEIIKAFVALRKGYEP
                     TDELKEEIRLFVKEGLSAHAAPREIEFKDKLPKTRSGKIMRRVLKAWELNLDAGDLST
                     ME"
BASE COUNT          587 a          445 c          245 g          683 t
ORIGIN      
        1 gtcatcattt tcaaatgctt tctacgaaat gatatgacac aagttaacag ctctttatat
       61 tgcttatcgt aaagtaaacg attatttaaa cattgacatg aaatatgttg tcacccttta
      121 ttcttcgaaa aaaatcttca ataacattca tgtcattatt ccattgtact taaatcccca
      181 gcatctaaat ttaattccca agcttttaat acacgtctca taattttacc tgaccgtgtt
      241 ttaggtaatt tatctttaaa ttcgatttca cgtggtgctg catgtgccga caaaccttct
      301 ttaacaaata aacgaatttc ttcttttaat tcgtctgttg gttcatatcc ttttctcagt
      361 gcaacaaacg ccttaattat ttcaccgcga accggatcag gtttaccaat aattcctgct
      421 tcggcaactg cttcgtgttc aaccaattta gactcaacct caaatggtcc aactcgttca
      481 ccagctgtca taattacatc atcaacacgt ccttggaacc agaagtaacc atcttcatct
      541 ttatatgccg aatcaccaga tacataccag tctccaataa aatatgattt atatttttct
      601 ggattcttcc agatacgata catcattgat ggccagcctt tttttatagc aaggttgccc
      661 attcgatttg gtggtaattc attacctgca tcatcgataa ttgcagcttg aataccaggt
      721 aatggtttgc ccattgagcc aagcttgacg tccatcgttg gatagttaac aatcatatgt
      781 ccacctgttt ctgtcatcca ccaagtatct aacaccgtta aaccgtatac ttttttcgcc
      841 cattttataa cttcaggatt taaaggctca cctactgata gaatcgaacg taacgatgac
      901 aagtcatatt tctcaacaat atcgtcacca gcactcatta acattcttaa agctgttggt
      961 gccgtatacc aaatcgtcac tttaaaatct tcaatcatac tataccactg ttctggtgaa
     1021 aagcgaccac cagctataca atttgtagcg ccatttaacc atggtgcaaa aataccataa
     1081 gatgttcctg taacccaacc tggatctgct gtacaccaat aaacatcatc ttcttgtaaa
     1141 tctaatacat attttccaga aatatagtgc actaacattg cttgttgaac atgcaataca
     1201 cctttaggtt gcccagtaga acctgatgta taatgtaaaa tcaaaccatc atccgacttt
     1261 aaccattcaa tgtcaaattc atcgctagca gtttccatca aactaatgaa gtctatgtaa
     1321 ttgtcttcta catcctcatc tacgacaaca atttttttca agttcggtaa tttatctaca
     1381 ggtactcgag gtaacaatgc cttattagta attaacactt tagcttcact gttctctaat
     1441 ctatccgcaa ctgccttttc cataaatgct tcaaataacg gcccaacaat tgcaccaatt
     1501 tttaaaacac ctaacaacgc aaaatatagt tcaggtgtac gcgacataaa tataaatact
     1561 ctgtcacctt tgtcaacttc tgcatgttca gacaaaacat tcgctgcttt attagataac
     1621 cgttgcatat ctttataagt atacgattct tttctgtact catctttgta atttaacgct
     1681 attttatccc ctaatccttg atctacatgg cgatctatac attcatatgc catgttcatt
     1741 tttccagttt cactccaaga aaatgcttgt tctacgtctt tccaatcaaa agtattatat
     1801 gtttcttcat aatctttaag gttatgttta ccttgcgctc ctttataaac ttcgactttc
     1861 atttgaaact ccccctttgt tatgtgaaaa cgctttcttt ctcaatatta tacataacat
     1921 ttctaattat tcaaaatttt tacttttatc ctttttctag
//
LOCUS       DANBWH010000305         1955 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.315, whole genome shotgun sequence.
ACCESSION   DANBWH010000305 DANBWH010000000
VERSION     DANBWH010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.315"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..169
                     /locus_tag="PPY92_002677"
     CDS             <1..169
                     /locus_tag="PPY92_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626612.1"
                     /translation="MLVNKINILYSDNEGDGTMTFVTGTEYFKWYFPEYPDKIKTLKI
                     EDVKKHTFTDK"
     gene            1095..>1955
                     /locus_tag="PPY92_002678"
     CDS             1095..>1955
                     /locus_tag="PPY92_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-hydroxamate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDJ2626613.1"
                     /translation="MKRLIGILLCSLFILTACSTSVDKTSDSTKTIDYKIENGKTLKV
                     PEKPKRVAVLTGFYVGDFIKLGIKPIAVSDITKDSSILKPYLKGVDYIGENDVEKVAK
                     AKPDLIVVDAMDKNIKKYQKIAPTVPYTYNKYNHKEILKEIGNLTNNEDKAKKWIEEW
                     EDKTRKDKKEIQSKIGQATASVFEPDEKQIYIYNSTWGRGLDIVHDAFGMPMTKQYKD
                     KLQEDKKGYASISKENISKYAGDYIFLSKPSYGKFDFEKTHTWQNIEAVKKGHVISYK
                     AEDYWFTDPIT"
BASE COUNT          775 a          249 c          331 g          600 t
ORIGIN      
        1 tatgttagtt aataaaataa acatactata ctcagataat gaaggtgatg gaacaatgac
       61 atttgttact ggcacagaat attttaaatg gtattttcct gaatatccag acaaaataaa
      121 aaccttgaaa atagaagatg taaaaaaaca tacgtttaca gataaataaa tataaattat
      181 acgctgagaa acgtcctgtg ttgcagtaag ggataatatt gtgtaactag atatgtttat
      241 cgtaaatgtg gatccgtgaa atatgacttt aaacatcgct ggtcaatcta tctttgcgat
      301 tggtcgtaga ttaaaaccat atgaaacaaa atgacttagt gcatggggaa tttaggctat
      361 ggcttgaaaa ttggattaag ctaattatca gctaataaat ttatgaaaat agctgaaaat
      421 ccagaattaa atgtcccacc agtgtgatat atgggcgcaa gtattttata ctaataagca
      481 actcttttcg aaccagaacg caccaaagaa cacataacat caaacggcga aactaaaatg
      541 ccgtactaaa tgtttatgaa tttaacggaa gaactcaatt ttgagccttg taaaattaca
      601 taatttagtt atataaatgc tatttatggg tattatataa acgggagggg caacgttatt
      661 acttgcctat tagaacatgg aatggttctg ccccacctaa tcaggtatta ggtgacttat
      721 ggggagaaat cagttagaat gacatattca tgtctattta agcaggtgcg ttacacacct
      781 gctttctatt tacatttaaa gataaaatgt gctattattt tactagaact ttttaacatt
      841 tctctcaaga tttaaatgta gataacaggc aggtacttcg gtacttgcct atttttttat
      901 tcacaaacat ctatatcaaa aagctttcat gtgataataa aaattctctc acacgttaaa
      961 aacaatgtat aagttaatta tggcttaacg aaatacattc attccatcga gtaggatagg
     1021 tataagtatt gactattatt tttaaaaatc tataattgaa aatgaatatc aattaaaagc
     1081 gaaaggatga tattatgaaa agattaattg gaattttatt atgtagttta tttattttga
     1141 ctgcgtgttc aacatctgtc gataaaacaa gtgactcgac aaagactata gactataaaa
     1201 ttgaaaatgg taaaacactg aaagtaccag agaaacctaa aagagttgct gtattaactg
     1261 gattttatgt tggtgatttt ataaagttag gaatcaaacc aattgctgtt tcagatataa
     1321 ctaaagattc ttcaatttta aaaccttatt taaaaggggt tgattatatt ggagaaaatg
     1381 atgttgaaaa agttgctaaa gcaaaacccg atttaatcgt tgtagatgct atggataaaa
     1441 atattaaaaa ataccaaaaa atagcgccaa cagtcccata tacatataat aaatacaatc
     1501 ataaagaaat attaaaagaa ataggcaatt tgactaataa tgaggataaa gcaaaaaaat
     1561 ggattgaaga gtgggaagat aaaactagga aagataaaaa agaaattcaa agtaaaattg
     1621 gtcaagcaac agcatctgtg tttgaaccag atgaaaagca aatatacata tataactcta
     1681 catggggtcg tggtttggat attgttcatg atgcattcgg tatgccaatg acaaagcaat
     1741 ataaagataa attacaagaa gataaaaaag gttatgcttc gatttcaaaa gaaaatatta
     1801 gtaaatatgc tggtgattat atatttttaa gtaagccttc atacgggaaa tttgattttg
     1861 aaaaaacaca tacatggcag aatattgaag ctgtaaaaaa aggacatgta atttcatata
     1921 aagcagaaga ttattggttc acagatccta ttaca
//
LOCUS       DANBWH010000306         1938 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.317, whole genome shotgun sequence.
ACCESSION   DANBWH010000306 DANBWH010000000
VERSION     DANBWH010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.317"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            144..488
                     /locus_tag="PPY92_002679"
     CDS             144..488
                     /locus_tag="PPY92_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HDJ2626614.1"
                     /translation="MNRNRMKQIILEYIKNNDSTSFVEIENVFEEQGFKYKGNGAYTS
                     GNHKNIIFWVGWNEEAFNIVADLKRDGLIEMQICPPMYYLIDGKGLKLPIVKNKNIKI
                     DHWLPVTFSLVN"
     gene            713..919
                     /locus_tag="PPY92_002680"
     CDS             713..919
                     /locus_tag="PPY92_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626615.1"
                     /translation="MLDHTNESVKRVFKYRDKQELLNKYHELLDKYFLHNISFYEEDK
                     NNERIYGLVVHTKNKLGDEDYERV"
     gene            906..>1938
                     /locus_tag="PPY92_002681"
     CDS             906..>1938
                     /locus_tag="PPY92_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HDJ2626616.1"
                     /translation="MKEFKEQFGYQLSNFDDMDIKGYANLYQKDIGKDVSMIEQGLKQ
                     LSITETEVLLPEQINRKLLGVLNMNEVNQSSNTWVGTLTKQLVSSENNFNIQELPTAR
                     KEVFKELLVNCELPQSLREVITITDDEHVESIPSLTYIKDKLATNGIELSLNGSSKYF
                     DRREGHIYTEVSDSVVHGSDRTLDDLLKEIFINECVAYETTLLLDKNNSSGLIDKGNQ
                     DLSLYNQGIKEVSNTSIYDGIKQAMKDIPQTFRRNVSIVMNTEHHDKLIKELAQMGLG
                     GLAGDLSNLFNVTNVVVTDDAHDVFVGDFGHAIYAKYEPIMYNKKKQALKGIYQFALN
                     YVFDIKIIPE"
BASE COUNT          753 a          239 c          368 g          578 t
ORIGIN      
        1 attttcagtc actcagtaac caacagcctg tatgtattga catcactgtg tttacagtgg
       61 ttattgaaaa acataaattc agtggttttt agtatctaac gtggttgctt tagtaactgg
      121 atagattgga ggacgagagt aacatgaata gaaatagaat gaaacaaatc attttagaat
      181 atataaagaa caatgacagc acttcgtttg tagagattga gaatgtattt gaagaacaag
      241 gttttaaata caaaggtaac ggcgcatata ccagtggcaa ccataagaat attatatttt
      301 gggtgggttg gaatgaagaa gcgtttaata ttgtggcaga tttaaaacgc gacgggctaa
      361 ttgaaatgca aatttgtccg ccaatgtact atcttattga tggtaaaggt ctaaagctac
      421 caatcgtaaa gaataaaaat ataaaaatag atcattggct accagtaaca tttagtttag
      481 taaattaaaa catgcagata agcatttaaa atatcaagta tagatatggg agtgtgtata
      541 tttattaatc ataaaagaat cgcatatcaa ataatcaaac atatacctgt gaacgttcaa
      601 ttaatgaata cgaaatatat aaatcgcgtt gtaaggcgct atacaaaaaa gcgtgctgat
      661 tttgagctta ttaaaatgta tatctatcag attaaaagga gagaggcaag acgtgctaga
      721 tcatacaaat gaatctgtaa aacgtgtgtt taagtataga gataagcaag aactattaaa
      781 taagtatcac gaattattag acaaatactt tcttcataat atttcgttct atgaagaaga
      841 taaaaacaac gaacgtatat atggtttagt tgttcatact aaaaacaaat taggagatga
      901 agattatgaa agagtttaaa gaacaatttg ggtatcaact atctaacttt gatgatatgg
      961 atattaaagg atacgccaat ctatatcaaa aggatatagg taaagatgta agtatgattg
     1021 aacaggggtt aaagcaatta agtattactg aaacagaagt tttattacca gagcaaatta
     1081 acagaaaatt attaggagtg ttaaatatga atgaagtaaa tcaatcaagt aatacatggg
     1141 taggtacctt aacaaaacaa ttagttagta gtgagaataa ctttaatatt caagagttac
     1201 caactgcacg taaagaagta tttaaagagt tattggtgaa ttgtgagcta cctcaatcac
     1261 tacgtgaggt gattaccatt acagatgatg aacatgttga atctatacca tcactaacat
     1321 acattaaaga taagttagct actaacggta ttgagctatc acttaatggc agtagtaaat
     1381 actttgatag acgtgaggga catatctaca cagaagtatc agacagtgta gttcatggct
     1441 ctgatagaac attagatgat ctacttaaag agatatttat taatgagtgt gtcgcatatg
     1501 aaacaacatt gttattagat aagaataatt caagtggttt aattgataaa ggtaaccaag
     1561 acttatcact ttataatcaa ggtatcaaag aagtaagtaa cacatcaata tacgatggta
     1621 tcaagcaagc gatgaaagat ataccacaaa cgtttaggcg taatgtatct attgtgatga
     1681 acacagaaca tcatgataag ctcatcaaag agttggcaca gatgggatta ggtggacttg
     1741 caggtgattt aagtaaccta tttaatgtga ctaatgtagt ggttactgat gatgcgcatg
     1801 atgtctttgt tggagacttt ggacatgcca tatatgctaa atacgaacct attatgtata
     1861 acaaaaagaa acaagcatta aaaggtattt atcaatttgc attgaactat gtgtttgata
     1921 taaaaataat tccagagt
//
LOCUS       DANBWH010000307         1928 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.318, whole genome shotgun sequence.
ACCESSION   DANBWH010000307 DANBWH010000000
VERSION     DANBWH010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.318"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..365
                     /locus_tag="PPY92_002682"
     CDS             <1..365
                     /locus_tag="PPY92_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HDJ2626617.1"
                     /translation="LDNVEDEKLKQKIENFKFFSQYADFKDLKNYQDGSITTNENVPR
                     YEAEYKLNNSDTNVKKLRDIYPITTKKAPILKLHIDGDIKGSSVGYKKIEYKFSKVKD
                     QETTLRDYLNFGPSDEDS"
     gene            413..1198
                     /locus_tag="PPY92_002683"
     CDS             413..1198
                     /locus_tag="PPY92_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDJ2626618.1"
                     /translation="MGYSKRFALYISVMILIFAIAGCGKSDETKEGSKEEQIKKSFAK
                     TLDMYPIKNLEDLYDKEGYRDGEFKKGDKGTWVISSVMVKQPKGEIMKSRGMYLFLNR
                     NTRTAKGYFIVDETSNDTLKKTEDKEKRYPVKMVNNKIVPIDPINDKGIKKEIENFKF
                     FSQYGDFKELKNYKNGEVSYNSEAPIYSAKYQLKNNDYNVKQLRKRYDISTEKAPKLL
                     LKGTGDLKGSSIGHKDIEFTFVENQEENIFFTDSLEFTSSENY"
     gene            1318..1928
                     /locus_tag="PPY92_002684"
                     /pseudo
     CDS             1318..1928
                     /locus_tag="PPY92_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835976.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
BASE COUNT          828 a          198 c          371 g          531 t
ORIGIN      
        1 ttttggataa tgtagaagac gaaaaactta aacaaaaaat tgaaaatttt aaatttttta
       61 gtcagtatgc cgattttaaa gatttaaaaa attatcaaga tggaagtata acaactaatg
      121 aaaatgtacc gagatatgaa gcagaataca aattgaataa tagtgataca aatgtaaaaa
      181 aacttagaga tatttatcca attacaacga aaaaggctcc aatattaaag ttacatatag
      241 atggtgatat aaaaggaagt tcagttggat acaaaaaaat agaatataaa ttttcaaaag
      301 ttaaagatca agagacaaca ttaagagatt atttaaattt tgggccgtct gatgaagata
      361 gctaaagttt attagatgta gaaataattc taaagcagag agacgtgaaa tgatgggata
      421 ttcaaaaagg tttgcattgt acataagcgt tatgatttta atatttgcga tagcaggttg
      481 tggcaaaagt gatgaaacaa aagaaggttc caaagaagaa caaattaaaa agagctttgc
      541 gaaaacgtta gatatgtacc caattaagaa tctcgaggac ttatatgaca aagaaggata
      601 tcgagatggc gaatttaaaa agggtgacaa agggacatgg gttatcagtt cagtaatggt
      661 taaacagcca aaaggtgaaa ttatgaaatc aagagggatg tatctatttt taaatagaaa
      721 tacaagaacc gcaaaaggat attttattgt agatgaaaca agtaatgata ctttaaaaaa
      781 aacagaggat aaagaaaaac gttatccagt aaagatggtg aataataaaa tagtacctat
      841 tgatccaatt aatgataaag gtataaaaaa agaaatagaa aattttaagt ttttctcaca
      901 atatggtgat tttaaagaat taaaaaatta taaaaatgga gaggtttcat acaattctga
      961 agcccctatc tactcagcaa aatatcaact gaaaaataat gattataatg taaagcaatt
     1021 acgtaaaaga tatgatatat caacagaaaa ggctcccaaa ttgttgttga aaggtactgg
     1081 agatttaaaa ggttcatcaa tcggacataa ggacattgag tttacattcg tggagaatca
     1141 agaagagaat atatttttta ctgatagttt ggagtttacc tcaagtgaga actattagct
     1201 atgataaaaa aggggatacg gaacaatgaa taattttaga aaatgtgcgt tgtgtatagg
     1261 acgtcagtct taattctatt agcatcaggt tgcagtggcg tatttgacac tcctgaagat
     1321 tcaaaagaaa cacaaatcaa aaagagcttt gcgaaaacgt tagatatgta tccaattaaa
     1381 aatcttgagg atttatacga taaagaagga tatcgagatg gagaatttaa aaagggagac
     1441 aaagggacgt gggttgttag atctgaaatg ataattcaac caaaaggtaa aagtttaact
     1501 tcaagaggca tgatattata catgaataga aatactagga ccacaaccgg ttatttttca
     1561 atagaagaaa tagattcaag aaaaagcctt gatgaaagag aaacagaaaa aaagtatcct
     1621 gtgaaaatga taaacaataa aattattcca actgaggaga taaaagatga aaagttgaaa
     1681 aaggaaattg aaaactttaa gttttttgtg caatatggca gttttaaagg aatagagaat
     1741 tatgaaaatg gtgacatttc ctataattct gaagctccta tttattcagc gaaatataaa
     1801 ctgaaaaatg atgattataa tgttaaagaa ttacgaaaaa gatataatat tccaacagaa
     1861 aaggcgccta aattgttgtt gaaaggttcg ggggatttga aagggtcttc agttggatat
     1921 aaggaaat
//
LOCUS       DANBWH010000308         1927 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.319, whole genome shotgun sequence.
ACCESSION   DANBWH010000308 DANBWH010000000
VERSION     DANBWH010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.319"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..325)
                     /locus_tag="PPY92_002685"
     CDS             complement(<1..325)
                     /locus_tag="PPY92_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDJ2626619.1"
                     /translation="MLLCESKVINKNPKYRIIKYESEYLMIDLASNWIVFFFPFINWL
                     IPKTYVKITKNDYEKLNIVKPVKNKSIGWTIFAGIVLLGGTVRRNTYLFDFQLEKLIV
                     WSSCFI"
     gene            complement(326..1720)
                     /locus_tag="PPY92_002686"
     CDS             complement(326..1720)
                     /locus_tag="PPY92_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDJ2626620.1"
                     /translation="MSIDMYLDRSRNQASSVGNLSQTMNSNYDALEKAITQFINDDAL
                     KGKAYTSAKQFFSTVLIPLSTSMKTLSDLTKQACDNFVSRYTSEVDSISLKESELEED
                     IRSLSQQITRYENLTNNLKKHASDNQQAISSNQQIIQTLGQQKHELEEKLRKLREFNQ
                     KSPEIFKEVEEFQKIVQQGLTQAQNFWNFSTNQFNIPSGKELDWAKASHEKYLKVAMG
                     KIEHKAEKETLNKADFAVIKAYAKEHPEDDIPKSIMKYINDNKDSIKRDIGLDITSTL
                     LEQGGINASKFGVFINTAGGVKGPAGPNSFVEVKRTSGNVFIENGSKFAKGGKYLGKG
                     VAGVGFGIGMYDDLANDNKTFGEALSHNGMTLAAGSVAAFGVAALVSNPVGWAVVGIL
                     TAGIIGSTVSDYLYQNNYFGIKDKVDWAGHKIDEGIDAVNKTAEKAVDSVGNAIGDAT
                     KSISNHINPMKWSW"
BASE COUNT          575 a          371 c          252 g          729 t
ORIGIN      
        1 caataaaaca gctgctccaa acaattagtt tttctaattg aaaatcaaat aaataagtat
       61 ttcttcttac agtaccacca agtaacacaa tacccgcgaa tatagtccat cctatcgatt
      121 tatttttaac tggtttgaca atatttaatt tttcataatc attcttagtg attttgacat
      181 atgttttcgg tatgagccag ttaataaatg gaaagaagaa aacaatccaa ttacttgcta
      241 aatcaatcat taagtattca ctttcgtatt tgattattcg atatttagga tttttattaa
      301 taactttaga ttcgcaaagc aatatttacc aactccattt cataggatta atatgattac
      361 taatactttt tgtggcatca ccaatggcat taccaacact atctacagct ttttctgcag
      421 ttttgtttac agcatcaata ccctcgtcta ttttatgtcc tgcccagtcc actttatctt
      481 ttatgccaaa ataattattt tgataaagat aatcagaaac agtagatcca ataatacctg
      541 ctgttaaaat acctaccaca gcccaaccga ctggattact tactaaagcc gctacaccaa
      601 atgctgcaac agatccagct gcaagcgtca taccattatg cgacaatgcc tctccaaatg
      661 ttttattatc atttgcaaga tcatcataca tacctatacc aaatcctaca cctgcaacac
      721 ctttccctag atattttccg ccttttgcaa atttactacc attttctata aacacattac
      781 ctgatgtacg tttgacttcc acaaatgaat ttggaccggc tgggcctttc actccacctg
      841 ctgtattgat aaatacaccg aatttacttg catttatacc gccttgctct aaaagtgttg
      901 acgtaatatc taatcctata tctcgtttaa tactatcttt attgtcattt atatatttca
      961 ttatactttt cgggatatcg tcttctggat gttctttggc atatgccttt ataacagcaa
     1021 agtctgcttt atttaaagtt tctttctctg ctttatgttc aattttcccc atagcaactt
     1081 tcaaatattt ttcatgactt gctttagccc aatcaagttc tttacctgaa ggtatattaa
     1141 attgatttgt tgaaaagttc caaaaattct gcgcttgggt aagtccttgt tggacaattt
     1201 tttgaaattc ttcaacttct ttaaatattt ctggtgattt ttgattaaac tcacgtaatt
     1261 tacgtagttt ctcttctaat tcatgttttt gttgacctaa tgtttgtatt atttgttggt
     1321 tcgatgaaat ggcttgctga ttatcggaag catgcttttt caaattgtta gttaaatttt
     1381 catatcgcgt aatttgttga cttaatgatc tgatatcttc ttcaagctct gattctttta
     1441 aagatatgct atcaacctca ctcgtataac gtgacacaaa attatcgcaa gcttgcttcg
     1501 ttaaatcact caatgttttc atacttgttg ataatggaat taacaccgta ctaaaaaatt
     1561 gcttagctga cgtatacgct ttccctttaa gcgcatcatc attgataaat tgagtaattg
     1621 ctttttccaa cgcatcataa tttgaattca ttgtttgact caaattcccc acacttgaag
     1681 cttggtttcg agatctgtct aaatacatgt caatactcat cggcatgctc ctttttcaaa
     1741 aatatatgat tttcaaacta tttaaaatca aatgcttttt acatctacaa agttgtaaaa
     1801 ttttaaaact cggcgatgat tatttcttat gtaaaggagt ctagatgcag gtaaattgag
     1861 ataacatgtc gccttttttc ttattttagc atatggatat aatggtgtct ttgtatattc
     1921 gcaatta
//
LOCUS       DANBWH010000309         1915 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.320, whole genome shotgun sequence.
ACCESSION   DANBWH010000309 DANBWH010000000
VERSION     DANBWH010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.320"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            17..985
                     /locus_tag="PPY92_002687"
     CDS             17..985
                     /locus_tag="PPY92_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDJ2626621.1"
                     /translation="MKIGVFSVLFYDKNFEDMLDYVAESGLDMIEVGTGGNPGDKFCK
                     LDELLENEDKRQAFMKSITDRGLQISGFSCHNNPISPDPIEAKEADETLRKTIRLANL
                     LDVPVVNTFSGIAGSDDTAKKPNWPVTPWPTAYSEIYDYQWNEKLIPYWQDLAEFAKE
                     QDVKIAIELHAGFLVHTPYTMLKLREATNEYIGANLDPSHLWWQGIDPIAAIRILGQA
                     NAIHHFHAKDTYINQENVNMYGLTDMQPYGNVATRAWTFRTVGYGHSPYVWADIISQL
                     IINGYDYVLSIEHEDPIMSVEEGFQKACQTLKSVNIYDKPADMWWA"
     gene            complement(1346..1840)
                     /locus_tag="PPY92_002688"
     CDS             complement(1346..1840)
                     /locus_tag="PPY92_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HDJ2626622.1"
                     /translation="MILSILLIFFCIRLVSLKISINHSKQLKADGAVEYGVKNSKFLA
                     ITHVLIYVLAGVEAFINKDTFSFANGIGLVILIFAYIMLFMVIKTLGGIWTLKLFILP
                     NHPIIKSGLYKITKHPNYFLNIIPELIGVLLLTHATYTTILLVPYAYFLYVRIKQEEK
                     LMNI"
BASE COUNT          672 a          281 c          355 g          607 t
ORIGIN      
        1 gaaggagtgc ttttcaatga aaataggtgt attttcagta ttattttacg ataaaaattt
       61 tgaagatatg ttagattatg tcgcagaatc tggattggat atgattgaag ttggaacagg
      121 tggtaaccca ggagataaat tttgtaagtt agatgagttg ttagaaaatg aagacaagcg
      181 acaagcattt atgaagtcaa tcacagacag aggcttacaa ataagtggtt tcagttgtca
      241 taacaatcca atttctccag atccgataga agcgaaagaa gccgatgaaa cgttacgtaa
      301 aacaatccgt ttagcaaatc tattagacgt gccagttgtt aatacatttt ctggcattgc
      361 agggtcagat gataccgcta aaaagcctaa ttggcctgtt acaccttggc caacagccta
      421 ctctgaaatt tatgattatc agtggaatga aaagttgata ccatattggc aagatttagc
      481 tgagtttgca aaagagcaag atgtaaaaat tgccatagag ttacatgcag gatttttagt
      541 tcatacacca tatacgatgt taaagttacg tgaggctaca aatgaatata tcggtgctaa
      601 cttagatcct agtcatttat ggtggcaagg tattgatcca attgctgcga ttcgcatatt
      661 aggccaagca aatgcaattc atcacttcca tgctaaagat acgtatatta atcaagaaaa
      721 tgtaaatatg tatggtctaa ctgatatgca accatatggt aacgttgcga caagagcatg
      781 gacattccgt acagttggtt atggacatag tccatatgta tgggcagata tcataagtca
      841 acttattatt aatggatatg attatgtatt aagtattgaa catgaagatc ctattatgtc
      901 agtagaagaa ggtttccaaa aagcttgtca aactttgaaa tctgttaata tttacgacaa
      961 gccagcagac atgtggtggg cataatacga actcgaggtt agtctgaagt ttgtctgaag
     1021 taagactggt ggcagtgttg aatgaatgca tatgtcgcca agccattgcc aaaaatttca
     1081 caccttaaat catgtcattg tttgtaaaga aggtgtactt tatataagtg tttagcgatg
     1141 gtcataccca ttaacagtaa caatcctcac cattgaaaag agtatataac cttttcgata
     1201 gtgaggtata tgataataaa aaaagcctgt tgtcacaatg gtcatagaca cgacatactt
     1261 taaaggcttc tgaatataat atttcagaac gcactttaaa gatggacgtc gatgtcgact
     1321 aatgtgatga caggctttca tctttttaaa tattcattaa tttctcttct tgtttaatac
     1381 gtacatataa gaaatacgca tacggtacta ataaaatagt tgtatatgtt gcgtgtgtta
     1441 ataataatac accgattaat tcaggaatga tgtttaagaa gtaatttggg tgttttgtaa
     1501 ttttatataa tccagattta ataataggat ggttaggtaa aatgaataat tttaatgtcc
     1561 aaataccacc taaagtttta ataaccataa ataacatgat ataagcaaag attaatataa
     1621 ctaagccaat accatttgca aagctaaatg tatctttatt aataaatgcc tctacaccag
     1681 ccaatacata aattaaaacg tgtgttattg ctaaaaactt cgaattttta acgccatatt
     1741 caactgcacc gtctgctttt aattgttttg agtgattaat agatatcttt aagctgacaa
     1801 gtctgataca gaaaaagata agtaatatag atagaatcat gatgtcctcc gtcattatgt
     1861 catatgtata agcgttgatt ttgacaacat aaagtatttt atagataaaa cttgt
//
LOCUS       DANBWH010000310         1908 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.321, whole genome shotgun sequence.
ACCESSION   DANBWH010000310 DANBWH010000000
VERSION     DANBWH010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.321"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(83..1891)
                     /locus_tag="PPY92_002689"
     CDS             complement(83..1891)
                     /locus_tag="PPY92_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDJ2626623.1"
                     /translation="MHFHTHLLYNTCEKYCLGAVFFYFWGVFLYNSLHKCKGFSFHVL
                     LSLTEILKGCLATMDKELWIERANDSLVKHFYEQQSDIEQREGFESKLTFGTAGIRGK
                     FGLGEGRLNKFTIEKLALGLARYLNAQTNSPTIVIHYDIRHLSTEFAQIIANVLANHQ
                     ITVYLPDTYKTTPELSFAVRNLNTTAGIMITASHNPKDYNGIKVYGSDGAQLSTDASE
                     LASRYIEEVGDPLQIDIPISKQNTSYIKPFPKSVTDDYMKHIQNMIGYIPKSDLQVVF
                     TSLHGTSVPIVPELLKSLNFNQFNLVDAQCKPDPNFSSVQSANPEDHRAFDQAVELAN
                     KSHADLLISTDPDADRLGIAERDAHGHITYFNGNQIGALLLNYRIQQTSQLRHRLMIQ
                     SIVSSELTKSLARYNNVKYKEVLTGFKFIAQEIRQLDDHQNMIFAFEESYGFLSEPFV
                     RDKDAVQIVPLIIKYASELKLYGKTLKDELEQIYQTVGRHEDTLFSHTLEGLEGKKKI
                     ESIMTHFRSNPPQEIQGLKVKAIEDYLTSEVYHLDKDTTSQINSSKSNVIRVLFDEGF
                     IALRPSGTEPKIKLYVSLKCPDFDDVAQKINAMIFS"
BASE COUNT          634 a          326 c          306 g          642 t
ORIGIN      
        1 cgcgaaaaca taaggattct agtttcaaaa gggctgataa gtataaaatg aaatgtaata
       61 tttcgatgta taaaattttt aattagctaa aaatcatcgc attaattttt tgagctacat
      121 catcaaaatc cggacatttt aacgacacat ataatttaat tttaggttca gtaccagaag
      181 gacgtaaagc gataaatcct tcgtcaaata agacacgaat aacatttgat ttagaagaat
      241 taatctgtga cgttgtatct ttatccaaat gataaacctc gctagttaaa taatcttcaa
      301 ttgctttcac tttcagtcct tgaatctctt gcggtggatt tgaacggaaa tgtgtcataa
      361 ttgattcaat ttttttctta ccttctaatc cttctagcgt atgcgagaat aatgtatcct
      421 catgtctacc aacagtttga taaatctgtt ctaattcatc tttcaatgtt ttgccatata
      481 attttaactc agaagcgtat tttataatga gtggcacaat ttgtacggca tctttatcac
      541 gtacaaaagg ctctgataga aaaccgtaac tctcttcaaa tgcgaaaatc atattttgat
      601 gatcatccag ttgtcttatt tcctgagcaa taaatttaaa gcccgtcagt acctctttgt
      661 atttaacatt attatagcgt gctaatgact ttgttaactc actacttaca atagattgaa
      721 tcattaagcg gtgacgtaat tgagacgttt gttgaatacg ataattaagt aacagcgcac
      781 caatttggtt gccattaaaa tatgtgatat gaccatgtgc atcacgttca gcaataccaa
      841 gtcgatctgc atctgggtca gttgatatta ataagtctgc atgactttta tttgcaagtt
      901 cgactgcttg atcaaaagcg cgatgatctt caggattagc actttgaact gaactaaaat
      961 tcggatcagg tttacattgt gcatctacaa gattaaattg attgaaattc aaagatttta
     1021 ataactcagg tacaattgga acacttgtac catgcaaact agtgaacaca acttgtaaat
     1081 cagatttagg aatatagcca atcatatttt gaatgtgttt catatagtca tcagttacag
     1141 atttcggaaa aggtttgata taagatgtat tttgttttga aataggtata tcaatctgta
     1201 atggatcgcc aacttcttcg atataacgac ttgcaagctc agatgcatca gtcgataatt
     1261 gcgcgccatc agaaccatat actttaatgc cgttatagtc tttcggatta tgacttgctg
     1321 taatcataat gccggcagta gtattaagat tgcgaaccgc gaacgataat tccggtgtcg
     1381 ttttatatgt atcaggtaaa taaactgtta tttgatggtt tgctaataca ttagcaataa
     1441 tttgggcgaa ttcagttgaa agatgtctaa tatcataatg aatgactatt gttggactgt
     1501 ttgtttgggc atttaaataa cgcgctaaac ctaatgccaa tttttcaata gtaaacttat
     1561 taagtcgacc ttcaccaaga ccgaattttc cgcgtatacc cgcagtacca aatgttaatt
     1621 tactttcaaa accttctcgc tgttcaatgt cagattgctg ctcataaaaa tgtttaacta
     1681 aactatcatt agctctttct atccataatt ctttatccat tgttgctaaa catcctttca
     1741 aaatctcagt tagacttaat aaaacatgaa aactaaagcc cttacattta tgtaatgaat
     1801 tataaagaaa tacgccccaa aagtaaaaaa acacagcccc aagacaatac ttttcacaag
     1861 tattatataa tagatgtgta tgaaaatgca tggagtagat gtaagagt
//
LOCUS       DANBWH010000311         1891 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.322, whole genome shotgun sequence.
ACCESSION   DANBWH010000311 DANBWH010000000
VERSION     DANBWH010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.322"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..425
                     /locus_tag="PPY92_002690"
     CDS             <1..425
                     /locus_tag="PPY92_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDJ2626624.1"
                     /translation="FTSLILGTISGPTGALPALIIGAAPFYARLVEIAFKEIDKGVIE
                     AAWSMGANTWIVIRKVLLPEAMPALVSGITVTAIALVGSTAVAGVIGAGGLGNLAYLT
                     GFTRNQNDVILVSTVFILIIVFIIQFIGDWLTNKLDKR"
     gene            444..1265
                     /locus_tag="PPY92_002691"
     CDS             444..1265
                     /locus_tag="PPY92_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDJ2626625.1"
                     /translation="MKKLFGLILVLTFAVVLAACGNGNKSGSDDKKITVGASPAPHAE
                     ILEKAKPLLEKKGYDLDIKTINDYTTPNKLLDKGEIDANYFQHTPYLNTEKKDKGYKI
                     VSAGDVHLEPMAVYSKKYKSLKELPKGATVYVSNNPAEQGRFLKFFVDAGLIKIKKGV
                     KIEDAKFSDITENKKDIKFNNKQSAEFLPKIYQNEDADAVIINSNFAIEQKLNPKKDS
                     IAVESAKDNPYANLIAVKEGHQDDKKIKALIEVLQSKDIQDFINEKYNGAVIPAK"
     gene            1619..1813
                     /locus_tag="PPY92_002692"
     CDS             1619..1813
                     /locus_tag="PPY92_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDJ2626626.1"
                     /translation="MADESKFEQAKGNVKETVGNVTDNKNLENEGKEDKASGKAKEFV
                     ENAKEKATDFIDKVKGNKGE"
BASE COUNT          701 a          265 c          338 g          587 t
ORIGIN      
        1 catttacaag tttgatactt ggaacgataa gtggtccgac aggtgcgtta ccagccttga
       61 ttattggcgc agcaccgttt tatgcaaggc tcgtagaaat tgcttttaaa gaaattgata
      121 aaggtgtcat cgaagcggcc tggtcaatgg gtgccaatac ttggatagta attcgtaaag
      181 tcctcttacc tgaagccatg ccagcgctag tgtctggcat tacagttaca gcaatcgctt
      241 tagttggttc aacagcagtt gcaggtgtaa ttggtgccgg tggtttagga aatttagcat
      301 acttaacagg tttcactcga aatcaaaatg atgtcatttt agtatcaact gtttttattt
      361 taattattgt atttataatc caattcattg gggattggct tacaaataaa cttgataaac
      421 gataaattgg gggggttcat tttatgaaaa aattatttgg tcttatttta gtattaacat
      481 ttgcagttgt attagcagct tgcggtaatg gaaacaaaag tggcagtgac gataagaaaa
      541 taacagtagg tgcttcacca gcaccacatg ctgaaatttt agaaaaagca aaaccattat
      601 tagagaaaaa aggttatgac ctagatatta aaacaattaa cgattacact acgcctaata
      661 aattactaga taaaggtgaa attgacgcaa actatttcca acatacacca tatttaaaca
      721 cagagaaaaa ggataaaggt tacaaaatcg taagtgccgg tgatgttcac ttagaaccta
      781 tggctgtata ctctaaaaaa tataaaagtt taaaagaatt accaaaaggt gcgacagttt
      841 atgtgtctaa taatccagct gaacaaggac gcttcttaaa attcttcgtt gatgcaggtt
      901 taattaagat caaaaaaggc gtaaaaattg aagatgctaa gtttagtgat attacagaga
      961 ataaaaaaga tattaagttt aataataaac aatcagcaga attcttacct aaaatttatc
     1021 aaaatgaaga cgctgatgct gttatcatta attcaaactt tgcaatcgaa caaaaactaa
     1081 atcctaaaaa agattctatt gctgtagaaa gtgcgaaaga taatccttat gcaaacttaa
     1141 ttgctgttaa agaaggacat caagatgata agaaaatcaa agcattaatt gaagtattac
     1201 aatctaaaga tattcaagac ttcattaatg aaaaatacaa cggtgcagtt attcctgcta
     1261 aataaatcat attaatggct cagtaacgtg atgttgctgg gctttttaat ttaaacaggt
     1321 atttatatga tatttaggaa tgagatgcta ttacggataa agtaaaatcc tatatgcatg
     1381 cgtgctactc atttattctt atttttaatt tcacatggat agtgactact acatcataaa
     1441 tcattagtga ttctttatta tttcagtccc actccctcaa gttaatgatg aatctatttc
     1501 gctagttata aaatgacgtc aaatcaatat caaaaattat ttaagtaaaa tgtttagata
     1561 atttttcagt gggtaagtat tatatataac atttaattat tccgaggagg catttattat
     1621 ggcagacgaa agtaaatttg aacaagcaaa aggtaatgtt aaagaaacag taggtaatgt
     1681 tactgataat aaaaatttag aaaacgaagg taaagaagat aaagcttctg gtaaagcgaa
     1741 agaattcgtt gaaaatgcaa aagaaaaagc aactgatttt attgataaag taaaaggtaa
     1801 caaaggcgag taattttact tttttattcc taatacactg cacatgccac ttagtcggta
     1861 taaccatcgg ctaggtggtt tttgtattaa a
//
LOCUS       DANBWH010000312         1875 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.323, whole genome shotgun sequence.
ACCESSION   DANBWH010000312 DANBWH010000000
VERSION     DANBWH010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.323"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..395
                     /locus_tag="PPY92_002693"
     CDS             <1..395
                     /locus_tag="PPY92_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDJ2626627.1"
                     /translation="AWIANSISIIYLLVMGLSYFSLKSGNASQREELAKQLSQNGGNV
                     SLDMLQTTMGALAIILLISTLYGIFATICIKGRRKLSIILFVIAIIVSLMALNLIAIV
                     LWVIVMIMLISKKESKETTHKDDEYIYH"
     gene            complement(474..1826)
                     /locus_tag="PPY92_002694"
     CDS             complement(474..1826)
                     /locus_tag="PPY92_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDJ2626628.1"
                     /translation="MNNKRHSTNEQLSLDEINNTIKFDHRSSNKQKFLSFLGPGLLVA
                     VGYMDPGNWITSMQGGAQYGYTLLFVILISSLSAMLLQSMTVRLGIATGMDLAQMTRH
                     YLSRPIAIIFWIIAELAIIATDIAEVIGSAIALNLLFNIPLIVGALITVLDVFLLLFI
                     MKYGFRKIEAIVGTLIFTVLFIFIFEVYISSPQLNAVLNGFIPHSEIITNNGILYIAL
                     GIIGATIMPHNLYLHSSIVQSRTYSRHNNEEKAQAIKFATIDSNIQLSIAFVVNCLLL
                     VLGASLFFNSNADDLGGFYDLYHALKTEPVLGATMGAIMSTLFAVALLASGQNSTITG
                     TLAGQIVMEGFLRLHIPNWLRRLITRSLAVIPVIVCLIIFKGNAAKIEQLLVFSQVFL
                     SIALPFCLIPLQLATSNKDLMGPFYNKTWVNIISWTLIIILSILNVYLIVQTFQELQS
                     "
BASE COUNT          651 a          277 c          306 g          641 t
ORIGIN      
        1 tggcttggat tgcaaatagt attagtatta tttatttatt agttatgggg ctatcctatt
       61 tttctttaaa aagtggtaat gcatcacaac gtgaagaatt agcgaagcaa ttatctcaga
      121 acggtggcaa tgtttcttta gatatgcttc agacaacaat gggtgcatta gcaattattt
      181 tattaatttc aacactttat ggtatatttg cgacaatttg tattaaaggg cgtagaaaat
      241 tatcgattat actttttgtt atcgcgataa ttgtaagttt gatggctctt aatttaattg
      301 caattgtctt atgggttatc gtgatgatta tgttgatttc taaaaaagaa tcaaaagaaa
      361 ctacacataa ggacgatgag tatatttatc attaatatgt tcatagcaaa aaaaccatta
      421 aaatgttaag ttgttaatta ttagatacaa ccaacatttt aatggtttta tttttaactt
      481 tgtagttctt ggaatgtttg aacgattaaa tagacattta aaatacttag aataataata
      541 agtgtccatg aaatgatatt aacccatgtt ttattataga aaggtcccat caagtcttta
      601 ttactagtag ctaattgtaa tggtattaaa cagaatggca gagcaatact taaaaatact
      661 tgagagaata caagtagttg ctcaatttta gctgcattac ctttaaaaat gattaagcat
      721 acgataacag gaatgacagc aagggaacgt gtaattaaac gtcttaacca atttggtatg
      781 tgtaatctta aaaatccttc cattacaatt tgtcctgcta aagtaccagt aatcgttgaa
      841 ttttgacctg acgctaataa tgcaactgca aataatgtac tcatgattgc acccattgtt
      901 gcacctagta caggttcagt ttttaaggcg tgatataaat catagaaacc acctaaatcg
      961 tcagcgttcg agttgaaaaa tagtgatgct cctaacacta ataataagca attgactaca
     1021 aatgcgattg ataactgaat gttcgaatct atcgtagcaa atttaatcgc ttgcgccttt
     1081 tcttcattgt tatgtcttga gtatgttcta gattgtacaa ttgatgaatg taagtacaaa
     1141 ttatgaggca taattgtagc gccaataata cctaatgcaa tatagagaat gccgttattt
     1201 gtaatgattt cactatgtgg tataaatcca tttaacacag cattcaactg tggtgatgaa
     1261 atatagactt caaatataaa gatgaataac actgtgaaaa ttaatgtacc aacaatagct
     1321 tcaatttttc taaaaccata tttcattata aatagtagta aaaatacatc aagtacagtt
     1381 attagtgcac cgacgattaa aggtatgtta aataggagat taagagcaat agcactacca
     1441 ataacctcag caatatctgt agcgataatt gctagttctg caatgatcca aaagattata
     1501 gcaataggtc ttgataaata atgtcgtgtc atttgagcta agtccatacc tgttgctatt
     1561 cctaatctca ctgtcatgct ttgaagtaac attgctgata aacttgaaat aagaattacg
     1621 aatagcaaag tatagccata ttgggcgcca ccttgcattg atgttatcca gtttccggga
     1681 tccatgtaac caacagcgac taataaccca ggtccaagaa atgataaaaa tttctgttta
     1741 tttgaactgc gatgatcgaa tttaattgta ttgtttattt cgtctaaact taattgttca
     1801 tttgttgaat gtcgtttatt attcattttg actcacctct acttcttatg gacaatataa
     1861 taacctaata aaatg
//
LOCUS       DANBWH010000313         1864 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.324, whole genome shotgun sequence.
ACCESSION   DANBWH010000313 DANBWH010000000
VERSION     DANBWH010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.324"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..1049)
                     /locus_tag="PPY92_002695"
     CDS             complement(<1..1049)
                     /locus_tag="PPY92_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3578 domain-containing protein"
                     /protein_id="HDJ2626629.1"
                     /translation="MIDNFMQVLKLIKEKRTNNVVKKSDWDKGDLYKTLVHDKLPKQL
                     KVHIKEDKYSVVGKVATGNYSKVPWISIYDENITKETKDGYYLVYLFHPEGEGIYLSL
                     NQGWSKISDMFPRDKNAAKQRALTLSSELNKYITSNEFNTGRFYYAENKDSSYDLKND
                     YPSGYSHGSIRFKYYDLNEGFTEEDMLEDLKKFLELFNELASKVTKTSYDSLVNSIDE
                     IQEDSEIEEIRTAQKDKTLKEVEAPKGIIPKYKKGVSKTTKNDSEIEKSNKENKLTGK
                     VGEKLALNYFNELIDNKIDEDKKEQFRNILNDNPGSQHGHGYDLVAFDPTNTDKAVEK
                     FIEIKTSTSSSIEEP"
     gene            complement(1581..>1864)
                     /locus_tag="PPY92_002696"
     CDS             complement(1581..>1864)
                     /locus_tag="PPY92_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDJ2626630.1"
                     /translation="TDQAPSTKVAMAKVIKVFKLKPDCHCTSKYLNNLIEQDHRHIKV
                     RKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSSCHEISIMLAS"
BASE COUNT          560 a          324 c          231 g          749 t
ORIGIN      
        1 aatggttcct caatactaga agatgtagat gttttaattt caataaattt ttctacagct
       61 ttatctgtat ttgttggatc aaaagctact aaatcatagc catgaccgtg ttgagagcct
      121 ggattatcat ttaaaatatt cctaaactgt tctttcttat cttcgtctat tttattatca
      181 attagctcat taaagtaatt tagcgctaat ttttctccaa ctttaccggt taatttattc
      241 tctttatttg atttttcaat ttctgaatca tttttagtag tctttgatac acctttttta
      301 tattttggaa ttattccttt aggtgcttcc acttccttga gtgtcttatc tttttgtgct
      361 gttctaattt cttcaatttc gctgtcttcc tgtatttcgt ctatgctatt gaccaagcta
      421 tcataggatg tttttgtaac ttttgaagct aattcattaa atagttctaa aaatttcttt
      481 aaatcctcta gcatatcttc ttctgtgaat ccttcattca aatcataata tttgaatctt
      541 attgatccat gagaatatcc tgatggataa tcatttttta aatcataaga tgaatcttta
      601 ttttctgcgt aataaaatct tccagtatta aattcatttg atgtaatata tttattgagt
      661 tcggaagata aagttaatgc tctttgtttt gcagcatttt tatcccgcgg aaacatatca
      721 cttatctttg accatccttg attcaaagat aagtatatgc cttctccttc cggatgaaaa
      781 agatatacca aataatatcc atcctttgtt tcttttgtta tattctcatc atatattgaa
      841 atccaaggaa ctttactata gttcccagta gcaaccttcc ctacaactga atatttatct
      901 tcttttatat gcacttttaa ctgcttgggt aacttatcat ggactaaagt tttatataga
      961 tcacctttat cccaatcaga ttttttaact acattattgg tacgtttctc tttaattaat
     1021 ttaaggacct gcataaagtt gtctatcatt tgaaattccc tcctattata aaatatatta
     1081 tgtctcattt tcttcaatat gtacttattt atattttacc gtaatttact atatttagtt
     1141 gcagaaagaa ttttctcaaa gctagaactt tgcttcacta taagtattca gtataaagaa
     1201 tatttcgcta ttatttactt gaaatgaaag actgcggagg ctaactatgt caaaaatcat
     1261 gaacctcatt acttatgata agcttcttaa aaacataaca gcaattcaca taaacctcat
     1321 atgttctgat acattcaaaa tccctttatg aagcggctga aaaaaccgca tcatttatga
     1381 tatgcttccc cttatcaatg atattgctaa aaattcccta aacataattc tactttttta
     1441 ataatcgcta atgctcatat ttaacaactt atttaaatgg tacattctca cttatcatac
     1501 tctcacctat ataagtaata aggttctgtt gcaaagtaaa aaaatatagc taaccactaa
     1561 tttatcatgt cagtgttcgc ttaacttgct agcatgatgc taatttcgtg gcatgacgaa
     1621 aatccgtaga tctgaagaga cctgcggttc tttttatata gagcataaat acattcaata
     1681 ccttttaaag tattctttgc cgtattgata ctttgatatc ttgtctttct tactttaata
     1741 tgacggtgat cttgctcaat gaggttattc agatatttcg atgtacaatg acagtcaggt
     1801 ttaagtttaa aaactttaat tactttagcc attgctacct tcgttgaagg tgcctgatct
     1861 gtaa
//
LOCUS       DANBWH010000314         1851 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.325, whole genome shotgun sequence.
ACCESSION   DANBWH010000314 DANBWH010000000
VERSION     DANBWH010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.325"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            11..679
                     /gene="queC"
                     /locus_tag="PPY92_002697"
     CDS             11..679
                     /gene="queC"
                     /locus_tag="PPY92_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDJ2626631.1"
                     /translation="MESVLNNEKAIVVFSGGQDSTTCLFYAKKHFKEVELVTFNYGQR
                     HDTEIEVAKQIAQDQGMKHHVLDMSLLSQLTPNALTQHDMEITNNEDGIPNTFVPARN
                     LLFLSFAGALAYQIGAKHIITGVCETDFSGYPDCRDSFIKSMNVTLSLAMDKDFVIHT
                     PLMWLNKAETWKLSDELEVLDYIRTKTLTCYNGIIGDGCGECPACHLRQRGLNQYLES
                     KGAL"
     gene            681..1100
                     /gene="queD"
                     /locus_tag="PPY92_002698"
     CDS             681..1100
                     /gene="queD"
                     /locus_tag="PPY92_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDJ2626632.1"
                     /translation="MLQQIYPSTTHPYQFELNKDFNFSAAHHIPCEEAGICQNVHGHT
                     YFVNLTIVGDKLDDTGFLVNFSHLKKMIHGKFDHQLLNNLPAFKNKIPSTEIVAETIY
                     QIVKENLASLEHQPKCIQVFVRETPTSYVVFRPKEQV"
     gene            1104..1817
                     /gene="queE"
                     /locus_tag="PPY92_002699"
     CDS             1104..1817
                     /gene="queE"
                     /locus_tag="PPY92_002699"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDJ2626633.1"
                     /translation="MAKIPVLEIFGPTIQGEGRVIGRKTMFVRTAGCDYRCSWCDSAF
                     TWDGSAKGDIKLMTAEEIYDELKRIGGDLFNHVTISGGNPALIKGIQELVDLFQDKGI
                     FSALETQGSKFQPWMTQIDDLTISPKPPSSTMTPDLKKLDEVITQCVPSSLNLKVVVF
                     DDKDYDFAKMIHHRYPDIPFYLQVGNPYLSDSVDNHTEKLLERYEQLVDLVMQSNDMN
                     HVYVLPQLHTLLWSNKKGV"
BASE COUNT          653 a          306 c          330 g          562 t
ORIGIN      
        1 caattacaaa atggaaagtg tattaaataa tgaaaaagcc atcgttgtct ttagtggtgg
       61 tcaagacagt acaacatgtc tcttttatgc aaaaaaacat ttcaaagaag ttgaactcgt
      121 aacgtttaat tatggccaaa gacatgatac tgaaattgaa gtcgcaaaac aaattgcaca
      181 agatcaagga atgaaacatc acgttttaga tatgtcatta ttatcacaac ttactccaaa
      241 cgcattaaca caacatgata tggaaattac taataatgaa gatggtatac ctaatacatt
      301 tgttccagct agaaatttac ttttcttgtc gtttgcaggc gctctagctt atcaaattgg
      361 ggctaagcat attattacag gcgtatgtga aacagacttt tcaggctacc cagactgtcg
      421 cgatagtttt ataaaatcaa tgaacgtaac attaagccta gctatggaca aagattttgt
      481 cattcatact cctttaatgt ggttaaacaa agcagaaacg tggaaattaa gtgatgaact
      541 cgaagtttta gattatattc gtacaaaaac attaacatgc tataacggta tcattgggga
      601 tggctgtggt gaatgtccag cttgtcattt acgtcaacgt ggactaaatc aatatcttga
      661 aagtaaagga gcgctttaaa atgttacaac aaatctatcc tagtacaacg catccatatc
      721 aattcgaatt aaataaagat tttaattttt cggctgcaca tcacattcct tgtgaagaag
      781 caggtatttg tcaaaatgtc catggtcata cttactttgt taatttaaca attgtcggtg
      841 ataaactaga tgacactggc ttcttagtga actttagcca tttgaaaaag atgatacacg
      901 gtaaatttga ccatcaactg ttaaataact tacctgcttt taaaaacaaa atcccttcaa
      961 ctgaaatcgt agcggaaaca atttatcaaa ttgttaaaga aaatttggca tcgctcgaac
     1021 accaaccaaa atgtattcaa gtatttgtaa gagaaacacc aacaagttat gttgtattta
     1081 gaccaaagga acaggtgtaa ctaatggcta aaattcctgt actcgaaata ttcggtccaa
     1141 ccattcaggg tgaaggtcga gtaatcggta gaaaaacgat gtttgtgaga acagctggat
     1201 gtgattatcg ctgtagctgg tgtgattctg catttacttg ggatggtagt gctaaaggcg
     1261 atataaaact catgaccgct gaagaaattt atgatgaatt aaaacgaatt ggtggcgatt
     1321 tatttaatca cgttacaatt tctggtggta acccagcatt aattaaaggt atccaagagt
     1381 tagttgactt atttcaagat aaaggtattt tcagcgcact ggagacacaa ggcagtaaat
     1441 tccaaccttg gatgacacaa attgatgatt taacgattag tccaaaacca ccaagttcaa
     1501 ctatgacacc tgatttaaaa aagttagatg aagtgattac acaatgcgta ccttcctcat
     1561 taaacttaaa agtagttgta ttcgacgata aagattatga ttttgccaaa atgatacacc
     1621 accgttatcc agatattccc ttctatttac aagttggtaa cccatattta tcagacagcg
     1681 tagataatca taccgaaaag ttgttagaac gctacgagca gttggtggat ttagttatgc
     1741 aaagtaatga tatgaatcac gtttatgtgt taccacaact tcatacatta ctttggagta
     1801 ataaaaaagg tgtataaagg ataacttatt ttacttttta caagtcatat c
//
LOCUS       DANBWH010000315         1843 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.326, whole genome shotgun sequence.
ACCESSION   DANBWH010000315 DANBWH010000000
VERSION     DANBWH010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.326"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            15..614
                     /locus_tag="PPY92_002700"
     CDS             15..614
                     /locus_tag="PPY92_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDJ2626634.1"
                     /translation="MAFKLPNLPYAYDALEPYIDQRTMEFHHDKHHNTYVTKLNATVE
                     GTELEHQSLADMIANLDKVPEAMRMSVRNNGGGHFNHSLFWEILSPNSEEKGGVIDDI
                     KAQWGTLDEFKNEFANKATTLFGSGWTWLVVNDGKLEIVTTPNQDNPLTEGKTPILLF
                     DVWEHAYYLKYQNKRPDYMTAFWNIVNWKKVDELYQAAK"
     gene            981..1706
                     /gene="sasD"
                     /locus_tag="PPY92_002701"
     CDS             981..1706
                     /gene="sasD"
                     /locus_tag="PPY92_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070002951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasD"
                     /protein_id="HDJ2626635.1"
                     /translation="MKKLATVGSLIVTSTLVFSSMPFQNAHADTTSMNVPNKQSQNVQ
                     NHRPYGGVVPQGMTQAQYTELEKTLPQLSAGSNMQDYNMKLYDATQNIADKYNVIITT
                     NVGVFKPHAVRDMNGHALPLTKDGNFYQTNVDANGVNHGGSEMVQNKTGHMSQQDHMN
                     QNTHMNQQPQIQQGHMQSSNHQMMSPKANMHSSNHQMNQSNKKVLPAAGESMTSSILT
                     ASIAALLLVSGLFLAFRRRSTNK"
BASE COUNT          710 a          285 c          323 g          525 t
ORIGIN      
        1 ggaggaatat acttatggca tttaaattac caaatttacc atatgcatat gatgcattgg
       61 aaccatatat agatcaaaga acaatggagt ttcatcacga caaacatcac aatacgtacg
      121 tgacgaaatt aaacgcaaca gttgaaggaa cagagttaga gcatcaatca ctagcggata
      181 tgattgctaa cttagacaag gtaccggaag cgatgaggat gtcagtccgt aataatggcg
      241 gtggtcattt taaccattca ttattctggg aaatactatc acctaattct gaagaaaaag
      301 gtggcgtaat agatgacatc aaagcgcagt ggggcacttt agatgaattt aaaaatgaat
      361 ttgcaaataa agcaacaaca ttatttggat caggttggac ttggttagtt gttaatgatg
      421 gaaaattaga aattgtgaca acgccaaacc aagataatcc attaacagaa ggcaaaacac
      481 caatcttact atttgatgtt tgggagcatg cctactatct gaaatatcaa aataaacgtc
      541 cagactatat gactgcattt tggaatattg ttaactggaa aaaagttgat gaattatacc
      601 aagcagcaaa ataatataac ttaatataat tgaggtggag catttacaag gtgttccact
      661 tttttgtact taatatcttt acttatttgt ttttgtgtaa tgggatagca cgtaaaagtg
      721 gcaaaataat aagataaatt tcaactcgaa ataccttatt attgttgact ataatgatga
      781 cctaaatgtt atatacatca gacaacacta ttaattaatc aacatattga cattaaataa
      841 attgacaaaa taagtaatta ttgtgaatct aaagtgaaat ttttataaaa aattgtaacg
      901 attcaaaaat tttgttgcat ttcttttgta atcatatgat aatgtaaatg taatcaaatt
      961 gtaatataag ggaaaagaca atgaaaaaat tagcaacagt aggttcttta attgtaacaa
     1021 gcactttagt attctcaagt atgccttttc aaaatgcgca tgccgacacg acttcaatga
     1081 atgtgccgaa taaacaaagc caaaatgtac aaaatcatcg cccttatggc ggagtagtac
     1141 cacaaggaat gacgcaagca caatatactg aattagagaa aactttaccc caattaagcg
     1201 ctggcagtaa tatgcaagac tataatatga aattgtatga tgcgacgcaa aatattgctg
     1261 ataaatacaa tgtgataatt acaactaatg taggggtatt taaaccacat gctgttagag
     1321 atatgaatgg ccatgcgtta cctttaacaa aagatggcaa tttttatcaa acgaatgtag
     1381 atgcaaatgg tgttaatcat ggtggtagtg aaatggtgca aaataaaaca ggtcatatga
     1441 gtcaacaaga ccatatgaat cagaacacac acatgaacca acagccacaa atacaacaag
     1501 gtcatatgca atcatcaaac catcaaatga tgagtccaaa agcaaatatg cattcatcaa
     1561 atcatcaaat gaaccaaagt aacaaaaaag ttttaccagc tgctggtgaa agtatgacat
     1621 caagtattct tactgcaagt attgccgcac tactattagt atctgggtta ttcttagcat
     1681 ttagacgacg ttcaacaaat aaataaacat aatacgatta ataatagaaa aatcgtgtga
     1741 ttatctgagc gagcctagga cataaatcaa tgtcctaggc tcgctaatgt tatattggca
     1801 gtagttgact gaatgaaatt gcgcttgtaa caagcttttc cat
//
LOCUS       DANBWH010000316         1836 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.327, whole genome shotgun sequence.
ACCESSION   DANBWH010000316 DANBWH010000000
VERSION     DANBWH010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.327"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            79..498
                     /locus_tag="PPY92_002702"
     CDS             79..498
                     /locus_tag="PPY92_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosfomycin resistance bacillithiol transferase
                     FosD"
                     /protein_id="HDJ2626636.1"
                     /translation="MIQSINHICYSVSDLKNSIRFYKNILCGELLVSGKTTAYFNIGG
                     LWVALNEEKDIPRNEVQYSYTHVAFTIDESEFNDWYQWFKENDVNILEGRTRDVRDKQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKETKPHMVFYK"
     gene            510..956
                     /locus_tag="PPY92_002703"
     CDS             510..956
                     /locus_tag="PPY92_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002508778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDJ2626637.1"
                     /translation="MTRYTRLFEEKDFNQINKLLQLYYELGYPTTSKELIHRLSKINN
                     HKDYYILLLIEDEIIIGFSGMCRMMFYENDGNYMRILSFVVNSNFRGNGFGSLLLKES
                     EKLADKLNCKVITLNSGIRTERENAHKFYKANGFESKSYGFSKSIN"
     gene            1132..1740
                     /locus_tag="PPY92_002704"
     CDS             1132..1740
                     /locus_tag="PPY92_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDJ2626638.1"
                     /translation="MIIGYARVSSIDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRP
                     VFQEALNFVRMGDRFVVESIDRLGRDYDEIIETVSYLKEKDVQLMITSLPMMNEVIGN
                     PLLDKFMKDLIVQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQ
                     KRIIYHRVVEMLEEGKAISKIAKEVNITRQTVYRIKHDKGSS"
BASE COUNT          706 a          222 c          358 g          550 t
ORIGIN      
        1 atatataata agagttaaat caagtcgtag catccaaaaa gcaactatct caatatacta
       61 agatatagga ggcccaaaat gatacaatct atcaatcata tatgttattc cgttagtgat
      121 ttaaaaaatt cgatacgctt ttataaaaat attttatgtg gcgaattatt agtaagtgga
      181 aaaacaactg catatttcaa tattggtggc ttatgggttg cgttaaacga agaaaaagac
      241 attcctcgaa atgaagttca atattcgtac acacatgtag cgtttactat agatgaaagt
      301 gaatttaatg attggtatca atggttcaag gaaaatgacg tgaatatatt agaagggcgt
      361 actagagatg taagagataa gcaatcaatt tattttactg atcctgacgg acacaagtta
      421 gagttacata ctggcacact agaaaataga ttgaattatt ataaagaaac aaaaccgcat
      481 atggtatttt acaaataagt aggtgctcta tgacaagata tacaagattg tttgaagaaa
      541 aagactttaa ccaaatcaat aagttactac aactatatta tgagttagga tatccaacaa
      601 cgagtaaaga acttattcat cgattaagta agattaataa tcacaaagac tattatatac
      661 tactactgat tgaagacgag ataattatag ggtttagtgg catgtgcaga atgatgttct
      721 atgaaaatga tgggaattat atgcgtatat tgtctttcgt tgtcaattca aattttagag
      781 gaaatggatt cggttcatta ttgttaaagg aatctgaaaa gttagctgac aagttaaact
      841 gtaaagtaat tacattgaat agtggaatta gaacagaaag agaaaatgca cataagtttt
      901 ataaagctaa tgggtttgaa agtaaatctt atgggttttc aaaaagtatc aactaatgaa
      961 atttcatatt agcaataata gcttattagc caatcttaaa gcgcagtact tatatagtga
     1021 gtgtactgcg ctttctcatt tattgggtaa aattattctc atatatcgag taaagtaaca
     1081 cgcaattaga acgtatgatt agggtgtata ttaattatgg agtgaagatg aatgattata
     1141 ggttatgcga gagtatcatc gatagatcaa aatttagaaa gacaattgga taatttaaag
     1201 acgtttggtg tggagaaaat ctttacagag aaacagtcgg gaaagtcagt ggaaaataga
     1261 cctgtatttc aagaagcact caattttgtc agaatgggag atcgattcgt tgtggaatct
     1321 attgatcgtt tgggacgtga ttatgatgaa attattgaaa ctgtgagtta tttaaaagag
     1381 aaagacgttc agttaatgat taccagctta cctatgatga atgaagtgat tggcaatcca
     1441 ttactggata agtttatgaa agatttaatc gttcaaattt tagcaatggt ttcagaacaa
     1501 gagagaaatg aaagtaaacg tcgacaagcg caaggaattc aagttgcgaa ggaaaaaggc
     1561 gtatataaag ggcgcccctt actttattca ccaaatgcca aagacccaca gaaacgtatt
     1621 atttatcatc gcgtggtgga aatgttagaa gaagggaaag caattagtaa gattgcgaaa
     1681 gaagttaata tcacaagaca gacagtttat agaattaagc atgataaggg atcatcttga
     1741 taaatgggtc gtcaatgcaa ataagaggct ctcacagagt ctcaaaactc atattatatt
     1801 attgaaatga tcttttaata attatacaag aaaaaa
//
LOCUS       DANBWH010000317         1833 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.328, whole genome shotgun sequence.
ACCESSION   DANBWH010000317 DANBWH010000000
VERSION     DANBWH010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.328"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            283..1656
                     /locus_tag="PPY92_002705"
     CDS             283..1656
                     /locus_tag="PPY92_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HDJ2626639.1"
                     /translation="MTAETNYFWLNCGYNRWNHNEPLVGQTALFESGAHFNPSQGFRA
                     FKKAKIGDQVIFYQVQTDTGLLGCGEIISVETGAQNKIRVQFRFNEQLKPLTADYLKR
                     SEALEFRMSNMKETLFNQITAEEFDLISGLGKGEIKIPRYFFLAETEEFEPGNQYTIY
                     THTYNGIKRNGYHFYTQLEEGDNIIFYNRTKNQSVVGIGEVSKHIHEKPPIPGRTNST
                     VIEVSYEKDITPITLSTLNKHPKLKNLYFLQENAKQAIASMSQAQYDAIIEMSDNNGL
                     KSPFEMVKKPDMLESEKEEALKPFILLVVDRKEEGLKAANDLLQKANANPVITTGHPD
                     FSEDMLYGKYLPNETGALYYREGFITQLMPKKDKSYLVIDNFNRIDTDIFQTYINVLE
                     GYEVTLPRYNKDGNMIKWSRQKDSFYYFNPNWHIVGITYDSLEEIKEKYSEQFLKYTR
                     IVKVKHD"
BASE COUNT          692 a          240 c          346 g          555 t
ORIGIN      
        1 aactgattaa aaggtataca gttgctcgta tgtgtgtcca actgtattta gagtcgcact
       61 tgttgtaaag caaaatagtt cgacttactt agaagtgaaa acgataaggg tctgggacgt
      121 aaattaatgt cctgggcgct acaatgttat aggaaatgcg attaaaatcg cagtgaacaa
      181 agggtataca gttgttcgca tacgcgtcca actgcataag aaacactaaa agaatgtaat
      241 tcatagtgtt tctaaaattt attcaggtta aggagtatta agatgacagc agaaacgaat
      301 tatttttggt tgaattgtgg atataaccgt tggaatcata atgaaccgtt agtaggacaa
      361 acagctttat ttgaatcagg cgcacatttt aacccatctc aaggatttag agcgtttaaa
      421 aaggctaaaa taggagatca agtcatcttt tatcaagttc agacggatac tggactactt
      481 ggatgtggtg aaattattag tgttgaaaca ggtgcacaaa ataaaatacg tgtacagttt
      541 cgttttaatg aacaattgaa accattaaca gctgattatt taaagcgtag tgaagctttg
      601 gaatttcgta tgagtaatat gaaagagaca ttgttcaacc aaatcactgc agaagagttt
      661 gatttaataa gtggtcttgg taaaggcgaa attaagatac cacgttattt ctttttagct
      721 gaaactgaag aatttgaacc tggaaaccaa tatacgattt atacgcacac gtacaacggt
      781 attaaacgta atggttatca tttttatact cagttagaag aaggcgacaa tattattttt
      841 tataatcgaa cgaaaaatca atctgtcgtt ggaattggag aagtttcaaa gcatattcat
      901 gaaaaaccac caattcctgg tagaacaaat agtacagtga ttgaagtgtc ttatgagaaa
      961 gacattacgc cgattacatt aagtacatta aataagcatc ctaaattgaa aaatttatat
     1021 ttccttcaag aaaatgcaaa acaggctatt gcaagtatgt cacaagcaca gtatgatgca
     1081 attattgaaa tgagcgataa taatggattg aaatctccat ttgaaatggt taaaaaacca
     1141 gatatgcttg aaagtgaaaa agaagaagcg ttaaagccat ttattttact tgttgtagat
     1201 cgaaaagaag agggcttaaa agcggctaat gacttattgc aaaaagcgaa tgcaaatcct
     1261 gttataacaa caggtcatcc tgatttttca gaagatatgc tttatggcaa gtatttacca
     1321 aatgaaacag gggcattata ctatcgtgaa gggtttatta ctcaattaat gcctaaaaag
     1381 gataaaagtt atttagttat tgataatttt aatcgtatag atacagacat tttccaaacg
     1441 tatattaatg tgttagaagg ctatgaagta acattaccac gttataataa agacggtaat
     1501 atgattaaat ggtcacgaca aaaagattct ttctattatt tcaatcctaa ttggcatatt
     1561 gtaggtatta catatgacag tttagaggaa attaaggaga aatattcaga gcagttttta
     1621 aaatacacac gtatagtcaa agtcaaacat gactaatgat atatggtagt ttttgaaaag
     1681 aagtaaggat aggattaaaa attgcataat tgcatatttg aataaccgca taaagaaaaa
     1741 tgacgggtaa tcaaccataa gagctaatta agagcttatg atagattgct cgtttttatt
     1801 ttaataaata ataaagacag tatatgaata taa
//
LOCUS       DANBWH010000318         1832 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.329, whole genome shotgun sequence.
ACCESSION   DANBWH010000318 DANBWH010000000
VERSION     DANBWH010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.329"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..609
                     /locus_tag="PPY92_002706"
     CDS             <1..609
                     /locus_tag="PPY92_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDJ2626640.1"
                     /translation="KSFIKIGLYFQIAYIVLMAITLCGFVICYGLIFGLFYLLSGSRA
                     DYLIVTIVISAIISIFVIILSIVPVIVLASDLFKERISKGVILIVLAIIALVLCNFVS
                     AILWFVSAISILGRKKLVAAADTTTIQKSKGNANQASHKDTCKKELDSQDMMEHPEVK
                     NPTTKNLEGFNEEIHKDEATTKVVSDNTEPPIESKGHVSKKD"
     gene            821..1597
                     /locus_tag="PPY92_002707"
     CDS             821..1597
                     /locus_tag="PPY92_002707"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HDJ2626641.1"
                     /translation="MTNNKVALVTGGAQGIGFKIAERLVEDGFKVAVVDFNEEGAKAA
                     ALKLSSDGTKAIAIKADVSNRDDVFNAVRQTAAQFGDFHVMVNNAGLGPTTPIDTITE
                     EQFKTVYGVNVAGVLWGIQAAHEQFKKFNHGGKIINATSQAGVEGNPGLSLYCSTKFA
                     VRGLTQVAAQDLASEGITVNAFAPGIVQTPMMESIAVATAEEAGKPEAWGWEQFTSQI
                     ALGRVSQPEDVSNVVSFLAGKDSDYITGQTIIVDGGMRFR"
     gene            1741..>1832
                     /locus_tag="PPY92_002708"
     CDS             1741..>1832
                     /locus_tag="PPY92_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626642.1"
                     /translation="MHQLKALLVLTHPRYYKTSQKHHYLIYLNK"
BASE COUNT          653 a          286 c          355 g          538 t
ORIGIN      
        1 aaatcattca taaagatagg tttatatttt caaatagctt atatagtact catggctata
       61 actttatgtg ggtttgtaat ttgctatgga ctaattttcg gccttttcta tttattatca
      121 ggtagcagag ctgattattt aatagtaaca atagttatat cggcaataat ttctatattt
      181 gtaattatac tttcaatcgt acctgtcatc gtattggcat ctgacttatt taaagaaagg
      241 atttcaaaag gtgtcatatt aattgtattg gctattattg ctttagtatt atgcaacttt
      301 gtatctgcaa tactctggtt tgtttcagcc atatctattt taggtagaaa aaaattagta
      361 gctgcagcag atactaccac tattcaaaaa agtaaaggga acgcaaatca agcatcacat
      421 aaagacacgt gtaaaaagga acttgatagt caagacatga tggaacatcc tgaggttaaa
      481 aatcccacga ctaaaaacct tgaaggattt aacgaagaaa tacataaaga tgaagctaca
      541 actaaagttg tcagtgataa cacggaaccg ccgattgaat caaaaggcca tgtctcgaaa
      601 aaagattgat gacaaactaa tcgagagact taaaaaaata atattcaaca taagaacttt
      661 taaaacgaca tttaaacgca ttgccaatca ctaatggtag tgcgtttaac tataccttaa
      721 atatctgaat attttgttaa atggagctac ctttgttgta ctattcaaat gaagaggagt
      781 aaaatgtaat taaaggaaag aaatttgagg agtgatcttt atgacaaaca acaaagtagc
      841 attagtaact ggcggagcac aagggattgg ttttaaaatt gcagaacgtt tagtggaaga
      901 tggtttcaaa gtagcagttg ttgatttcaa tgaagaaggg gcaaaagcag ctgcacttaa
      961 attatcaagt gatggtacaa aagctattgc tatcaaagca gatgtatcaa accgtgatga
     1021 tgtatttaac gcagtaagac aaactgccgc gcaatttggc gatttccatg tcatggttaa
     1081 caatgccggc cttggaccaa caacaccaat cgatacaatt actgaagaac agtttaaaac
     1141 agtatatggc gtgaacgttg caggtgtgct atggggtatt caggccgcac atgaacaatt
     1201 taaaaaattc aatcatggcg gtaaaattat caatgcaaca tctcaagcag gcgttgaggg
     1261 taacccaggc ttgtctttat attgcagtac aaaattcgca gtgcgaggtt taacacaagt
     1321 agccgcacaa gatttagcgt ctgaaggtat tactgtgaat gcattcgcac ctggtatcgt
     1381 tcaaacacca atgatggaaa gtatcgcagt ggcaacagcc gaagaagcag gtaaacctga
     1441 agcatggggt tgggaacaat ttacaagtca gattgctttg ggcagagttt ctcaaccaga
     1501 agatgtttca aatgtagtga gcttcttagc tggtaaagac tctgattaca ttactggaca
     1561 aacaattatt gtagatggtg gtatgagatt ccgttaataa tcatgcatta gcgataaata
     1621 aatccttatt gttaagttta atcacttagc agtaaggatt ttttagtgca cttagaaggg
     1681 attgtattgg tagaaaatta ataagcgaag ttcttaaatg agttatgatg tcacagtcta
     1741 atgcatcagt tgaaagcatt actagtatta acacacccaa gatattataa aacatcacaa
     1801 aaacaccact atctaattta tctcaataaa aa
//
LOCUS       DANBWH010000319         1819 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.330, whole genome shotgun sequence.
ACCESSION   DANBWH010000319 DANBWH010000000
VERSION     DANBWH010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.330"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(195..1130)
                     /gene="trxB"
                     /locus_tag="PPY92_002709"
     CDS             complement(195..1130)
                     /gene="trxB"
                     /locus_tag="PPY92_002709"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDJ2626643.1"
                     /translation="MTEIDFDIAIIGAGPAGMTAAVYASRANLKTVMIERGIPGGQMA
                     NTEEVENFPGFEMITGPDLSTKMFEHAKKFGAVYQYGDIKSVEDKGEYKVINFGNKEL
                     TAKAVIIATGAEYKKIGVPGEQELGGRGVSYCAVCDGAFFKNKRLFVIGGGDSAVEEG
                     TFLTKFADKVTIVHRRDELRAQRILQDRAFKNDKIDFIWSHTLKSINEKDGKVGSVTL
                     TSTKDGSEETHEADGVFIYIGMKPLTAPFKDLGITNDVGYIVTKDDMTTSVPGIFAAG
                     DVRDKGLRQIVTATGDGSIAAQSAAEYIEHLNDQA"
     gene            complement(1197..>1819)
                     /locus_tag="PPY92_002710"
     CDS             complement(1197..>1819)
                     /locus_tag="PPY92_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626644.1"
                     /translation="GKFLSIQMYNALAYNYYYLGEEDESHYYWDKLKQISKVEIGHAP
                     WVIENSKEVFDQHILPLLQSDDSHYRLYGIFLLDQLNGKEIVMTESIWQVLENLNNYE
                     KLYLTYLVQGLTLNKLDFIHRGLLTLYQNELFVSENDLMVAWINQGELIIAEKVDLTD
                     VEPYIGAFIYLYFKNQPRNVTKKQITTWLGITQYKLNKMIEFLLSI"
BASE COUNT          584 a          366 c          231 g          638 t
ORIGIN      
        1 taaagttata agtttaatat aacatataaa aaaaactaga actcattgaa acgcacaaat
       61 gatacgtact aagttctagt tttctatata atttgcttta atttaaaagt aagacattat
      121 tttaacaagg tgatgacact attaaaataa atatccaaat aacttacaac tcaacatctt
      181 aattcgacct cgaattaagc ttgatcgttt aaatgttcaa tatattccgc tgcactttgc
      241 gctgcaatac taccatcgcc agtagcagtg acaatttggc gtaaaccttt gtcacgaaca
      301 tctcctgctg caaaaatacc tggtactgat gttgtcatat catcttttgt cacaatataa
      361 ccaacatcat tcgtaatacc taagtcttta aatggcgctg ttaatggttt cataccaata
      421 tagatgaata caccatcagc ctcgtgtgtt tcttctgaac catcttttgt agacgttaat
      481 gtcacagaac ccactttgcc gtctttttca ttaattgatt tcaaagtatg actccaaata
      541 aagtcgattt tatcattttt gaatgctcta tcttgtaaaa tacgctgtgc acgtaactca
      601 tcacgacggt gaacgattgt tactttgtca gcaaatttag ttaagaatgt tccctcttct
      661 actgctgagt caccaccacc gataacgaat aggcgtttat ttttaaagaa tgcaccatca
      721 catactgcac aataacttac accgcgtcca ccaagttctt gttcacccgg aacaccaatt
      781 ttcttgtatt ctgcacctgt agcaataatg accgctttcg ctgttaattc tttattacca
      841 aagttaatca ctttatattc gcctttatct tctacagatt taatatctcc atattgataa
      901 actgcaccaa actttttagc gtgttcaaac atttttgtag ataaatctgg acctgtaatc
      961 atttcgaaac cagggaagtt ctctacttct tctgtattag ccatttgacc gcctggaata
     1021 cctctttcaa tcataactgt ttttaaatta gcacgtgatg cgtatactgc agcagtcata
     1081 ccagctggac ctgcaccgat aattgctata tcaaaatcta tttcagtcat tttattaacg
     1141 cctcctcatt attaatcatt atgcgcatta tataataaat ctaacttttc ataaatctat
     1201 atgctcaaga gaaattcaat cattttgttc agtttatatt gtgttatgcc taaccatgtt
     1261 gtaatttgct tctttgtaac gtttcgaggt tgatttttaa aatacaaata aataaacgca
     1321 ccgatatatg gctcaacatc agttaaatct actttttcag caattatgag ttcaccttga
     1381 ttaatccatg caaccattaa atcattttca cttacaaata attcattttg gtaaagcgtt
     1441 aacaagccgc gatgaatgaa gtctaattta ttgagcgtta aaccttgaac taaatacgtt
     1501 aaatacaatt tctcataatt atttagattt tccagaacct gccaaatact ttccgtcatc
     1561 acaatttctt taccatttaa ttgatccaat aaaaaaatac catataaacg ataatgactg
     1621 tcatcacttt gaagtaatgg caaaatatgt tggtcaaaaa cttctttgct attttcaatt
     1681 acccaaggcg catgtccaat ttccacttta gaaatttgct tcaatttatc ccagtagtaa
     1741 tgactttcgt cttcttcacc taaataataa taattatatg ctaaagcatt gtacatttga
     1801 attgataaaa atttccctt
//
LOCUS       DANBWH010000320         1790 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.331, whole genome shotgun sequence.
ACCESSION   DANBWH010000320 DANBWH010000000
VERSION     DANBWH010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.331"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            39..1298
                     /locus_tag="PPY92_002711"
     CDS             39..1298
                     /locus_tag="PPY92_002711"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDJ2626645.1"
                     /translation="MAQEVIKIRGGRTLNGEVNISGAKNSAVAIIPATLLAQGHVKLE
                     GLPQISDVKTLVSLLEDLNIKASLNGTELEVDTTEIQNAALPNNKVESLRASYYMMGA
                     MLGRFKKCVIGLPGGCPLGPRPIDQHIKGFKALGAEIDESSTTSMKIEAKELKGAHIF
                     LDMVSVGATINIMLAAVYATGQTVIENAAKEPEVVDVANFLTSMGANIKGAGTSTIKI
                     NGVKELHGSEYQVIPDRIEAGTYMCIAAACGENVILNNIVPKHVETLTAKFSELGVNV
                     DVRDERIRINNNAPYQFVDIKTLVYPGFATDLQQPITPLLFMANGPSFVTDTIYPERF
                     KHVEELKRMGANIEVDEGTATIKPSTLHGAEVYASDLRAGACLIIAGLIAEGVTTIYN
                     VKHIYRGYTDIVEHLKALGADIWTETV"
     gene            1387..1722
                     /locus_tag="PPY92_002712"
     CDS             1387..1722
                     /locus_tag="PPY92_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDJ2626646.1"
                     /translation="MEVCPYLEETFKILGRSWNGLIINYLSRCNDCSAHFSDMKRDLK
                     TITPRALSLKLSELAQWELVEKQIISTSPVQIIYVLTEKGKALAEALHPIEAWAQSYV
                     DLTDQRTAK"
BASE COUNT          635 a          243 c          367 g          545 t
ORIGIN      
        1 aaaaaattaa aaagtaatgg acaaaggaga taaatgatat ggctcaagag gtaataaaaa
       61 taagaggtgg acgcacacta aatggggaag ttaatatcag tggcgcgaaa aacagtgctg
      121 tagctatcat tcctgcaaca ttattagctc aaggacatgt gaaattagaa gggttaccgc
      181 aaatctctga tgttaaaact ttagtgagtt tattagaaga tttaaatatt aaggcatcac
      241 taaatggtac ggaattagaa gttgatacaa ctgaaataca aaatgctgca ttaccaaata
      301 ataaagttga atcattacgt gcttcatact atatgatggg tgccatgtta ggtagattta
      361 aaaaatgtgt gattggttta ccgggaggat gtccacttgg gccacgtcca attgatcaac
      421 atattaaagg atttaaagct ttaggtgctg aaattgatga atcaagcacg acatcaatga
      481 aaattgaagc taaagaatta aaaggtgcgc atatttttct agatatggtt agcgtaggtg
      541 caacaattaa cattatgcta gctgcagttt atgcaacagg acaaactgta attgaaaatg
      601 ctgctaaaga accggaagta gttgatgttg ctaacttctt aacaagtatg ggagctaata
      661 ttaagggagc gggcacatca acaattaaaa tcaatggtgt caaagaatta catggttctg
      721 agtatcaagt tatacctgat agaattgaag caggcacata tatgtgtatc gctgcagcat
      781 gtggagaaaa tgttatatta aataatattg ttccgaaaca tgtagaaaca ttaactgcta
      841 aatttagtga attaggtgtg aatgttgatg taagagacga aagaattcgc atcaataata
      901 atgcgccata tcaatttgtt gatattaaaa cactagtata tccaggtttt gctactgatt
      961 tgcaacagcc tattacacca ttattattta tggcaaatgg tccttcattt gtaactgata
     1021 cgatttatcc ggagcgtttt aaacatgttg aagaattaaa gcgcatgggt gcaaatattg
     1081 aagttgacga aggcacagca acgataaaac catcaacatt acatggtgct gaagtttatg
     1141 ctagcgattt aagagcagga gcttgtttaa ttattgctgg tttaattgct gagggtgtaa
     1201 ctacaattta caatgtaaaa catatttata gaggttatac agatattgta gaacacttaa
     1261 aagctttagg tgcagatatt tggacggaaa ctgtatagat ttatcttgtt ttgtggtata
     1321 ataattatac tgattaaaaa agggtgttcg tttacattct aaatttattg atgagaggtg
     1381 aagatgatgg aagtatgtcc gtatctcgaa gaaactttta aaatacttgg tagaagttgg
     1441 aatggattaa tcattaatta tctctcaaga tgtaatgact gttcagcaca cttttccgat
     1501 atgaaaagag atttgaaaac aataacacca cgtgctttaa gtcttaagtt gtcagagctt
     1561 gcacaatggg agttagttga gaagcaaatc atttctacga gtccagtaca aattatttat
     1621 gtgctaactg aaaaaggtaa agcgttagca gaggctttac atccaattga agcatgggcg
     1681 caatcatatg tcgatttaac agatcaacgt actgctaaat aattacatga cgtttaaaca
     1741 tagcattgat tataactatt tctaagtctt cgcattattt gcgatgatgt
//
LOCUS       DANBWH010000321         1777 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.332, whole genome shotgun sequence.
ACCESSION   DANBWH010000321 DANBWH010000000
VERSION     DANBWH010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.332"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            89..>1777
                     /gene="sasG"
                     /locus_tag="PPY92_002713"
     CDS             89..>1777
                     /gene="sasG"
                     /locus_tag="PPY92_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored surface protein SasG"
                     /protein_id="HDJ2626647.1"
                     /translation="MRDKKGPVNKRVDFLSNKLNKYSIRKFTVGTASILIGSLMYLGT
                     QQEAEAAENNIENPTTLKDNVQSKEVKIEEVTNKDTAPQGVEAKSEVTSNKDTIEHEA
                     SVKAEDISKKEDTPKEVANVAEVQPKSSVTHNAEAPKVRKARSVDEGSFDITRDSKNV
                     VESTPITIQGKEHFEGYGSVDIQKNPTDLGVSEVTRFNVGNESNGLIGALQLKNKIDF
                     SKDFNFKVRVANNHQSNTTGADGWGFLFSKGNAEEYLTNGGILGDKGLVNSGGFKIDT
                     GYIYTSSMDKTEKQAGQGYRGYGAFVKNDSSGNSQMVGENIDKSKTNFLNYADNSTNT
                     SDGKFHGQRLNDVILTYVASTGKMRAEYAGKTWETSITDLGLSKNQAYNFLITSSQRW
                     GLNQGINANGWMRTDLKGSEFTFTPEAPKTITELEKKVEEIPFKKERKFNPDLAPGTE
                     KVTREGQKGEKTITTPTLKNPLTGEIISKGESKEEITKDPINELTEYGPETIAPGHRD
                     EFDPKLPTGEKEEVPGKPGIKNPETGDVVRPPVDSVTKYGPVKGDSIVEKEEIPF"
BASE COUNT          732 a          248 c          360 g          437 t
ORIGIN      
        1 ttccattgtg caactcctat tgtatttgta aaattacatg taaattcact gtaagtaaag
       61 tggaaaatat ggaaaaagga gtatgcaaat gagagataag aaaggaccgg taaataaaag
      121 agtagatttt ctatcaaata aattgaataa atattcaata agaaaattta cagttggaac
      181 agcatctatt ttaattggct cactaatgta tttgggaact caacaagaag cagaagcagc
      241 tgaaaacaat attgagaatc caactacatt aaaagataat gtccaatcaa aagaagtgaa
      301 gattgaagaa gtaacaaaca aagacactgc accacaaggt gtagaagcta aatctgaagt
      361 aacttcaaac aaagacacaa tcgaacatga agcatcagta aaagctgaag atatatcaaa
      421 aaaggaggat acaccaaaag aagtagctaa tgttgctgaa gttcagccga aatcgtcagt
      481 cactcataac gcagaggcac ctaaggttag aaaagctcgt tctgttgatg aaggctcttt
      541 tgatattaca agagattcta aaaatgtagt tgaatctacc ccaattacaa ttcaaggtaa
      601 agaacatttt gaaggttacg gaagtgttga tatacaaaaa aacccaacag atttaggggt
      661 atcagaggta accaggttta atgttggtaa tgaaagtaat ggtttgatag gagctttaca
      721 attaaaaaat aaaatagatt ttagtaagga tttcaatttt aaagttagag tggcaaataa
      781 ccatcaatca aataccacag gtgctgatgg ttgggggttc ttatttagta aaggaaatgc
      841 agaagaatat ttaactaatg gtggaatcct tggggataaa ggtctggtaa attcaggcgg
      901 atttaaaatt gatactggat acatttatac aagttccatg gacaaaactg aaaagcaagc
      961 tggacaaggt tatagaggat acggagcttt tgtgaaaaat gacagttctg gtaattcaca
     1021 aatggttgga gaaaatattg ataaatcaaa aactaatttt ttaaactatg cggacaattc
     1081 aactaataca tcagatggaa agtttcatgg gcaacgttta aatgatgtca tcttaactta
     1141 tgttgcttca actggtaaaa tgagagcaga atatgctggt aaaacttggg agacttcaat
     1201 aacagattta ggtttatcta aaaatcaggc atataatttc ttaattacat ctagtcaaag
     1261 atggggcctt aatcaaggga taaatgcaaa tggctggatg agaactgact tgaaaggttc
     1321 agagtttact tttacaccag aagcgccaaa aacaataaca gaattagaaa aaaaagttga
     1381 agagattcca ttcaagaaag aacgtaaatt taatccggat ttagcaccag ggacagaaaa
     1441 agtaacaaga gaaggacaaa aaggtgagaa gacaataaca acaccaacac taaaaaatcc
     1501 attaactgga gaaattatta gtaaaggtga atcgaaagaa gagatcacaa aagatccgat
     1561 taatgaatta acagaatacg gaccagaaac gatagcacca ggtcatcgag acgaatttga
     1621 tccgaagtta ccaacaggag agaaagaaga agttccaggt aaaccaggaa ttaagaatcc
     1681 agaaacagga gacgtagtta gaccaccggt cgatagcgta acaaaatatg gacctgtaaa
     1741 aggagactcg attgtagaaa aagaagaaat tccattc
//
LOCUS       DANBWH010000322         1775 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.333, whole genome shotgun sequence.
ACCESSION   DANBWH010000322 DANBWH010000000
VERSION     DANBWH010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.333"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            25..1086
                     /locus_tag="PPY92_002714"
     CDS             25..1086
                     /locus_tag="PPY92_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDJ2626648.1"
                     /translation="MSRITQVHRILEQKHLDAIIILSDYNRRYLSGFTGTSGALIISK
                     DKQYLITDFRYIDQATKQAPNYEIINRKSTIIGEIKELLHQENFENIGFEGHHVSYDT
                     YLELNKSRISLISISNTVDKIRDVKDVDEIALIQKAANIVDETYEYILTVVKAGMTEK
                     ELKAILESKMLELGADGPSFDTIVASGHRGALPHGVASDKIIEKGDMITLDFGAYYNG
                     YCSDITRTFAIGEPDPKLKEIYQIVLESQMKAINEIRPGMTGAEADAISRNYLESKGY
                     GKEFGHSLGHGIGLEIHEGPMLARTIQDKLQVNNCVTVEPGVYIEGLGGIRIEDDILI
                     TENGCQVFTKCTKDLIVLT"
     gene            1112..1669
                     /gene="efp"
                     /locus_tag="PPY92_002715"
     CDS             1112..1669
                     /gene="efp"
                     /locus_tag="PPY92_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDJ2626649.1"
                     /translation="MISVNDFKTGLTISVDNAIWKVIDFQHVKPGKGSAFVRSKLRNL
                     RTGAIQEKTFRAGEKVEPAMIENRRMQYLYADGDNHVFMDNESFEQTELSSDYLKEEL
                     NYLKEGMEVQIQTYEGETIGVELPKTVELTVTETEPGIKGDTATGATKSATVETGYTL
                     NVPLFVNEGDVLIINTGDGSYISRG"
BASE COUNT          669 a          230 c          341 g          535 t
ORIGIN      
        1 aacgtttcac taggaggtaa taagatgagc agaataacac aagtgcatcg tattttagaa
       61 caaaaacatt tagatgcgat tatcattctt tcggattaca atagaagata tttatcaggt
      121 tttactggta ccagtggagc acttatcata tctaaagata aacaatattt gataacagac
      181 tttagatata ttgatcaagc aactaagcag gcgccaaatt atgaaattat taatcgtaaa
      241 tctactatta ttggtgagat taaagaattg ctacaccaag aaaattttga aaatataggt
      301 tttgaggggc atcatgtaag ttatgataca taccttgaat taaataaaag ccgtatatca
      361 ttaataagca tttctaatac tgtagataaa attagagacg tcaaagatgt tgacgaaatt
      421 gctttaattc aaaaagcagc taatattgtt gatgaaacat atgaatatat tttaactgtt
      481 gtaaaagcag gtatgactga aaaagaatta aaggcaatat tagaaagtaa aatgctagaa
      541 ttaggagcag atggaccatc atttgatacg attgtagcat ctggtcatag aggtgcatta
      601 ccacatggtg ttgcaagtga taaaattatt gaaaaaggcg acatgattac attagatttt
      661 ggcgcgtatt ataacggcta ttgttcagat attactagaa catttgctat tggagaacca
      721 gatcctaaac tgaaagaaat atatcaaata gtacttgaat ctcaaatgaa agcaattaat
      781 gagattagac ctggcatgac tggtgcagaa gctgatgcca tttcaagaaa ctatttagag
      841 tcaaaagggt atggaaaaga atttggacat tcactaggac atggtattgg tttagaaatc
      901 catgaagggc caatgctggc tcgtacgata caagataaac ttcaagttaa caactgtgtt
      961 acagtagaac ctggtgttta tatagaaggt ttgggcggta taagaataga agatgatatt
     1021 ttaattacag aaaatggttg tcaagtcttt actaaatgca caaaagacct tatagtttta
     1081 acataagcgt gtaaaatgag gaggaaactg aatgatttcg gttaatgatt ttaaaacagg
     1141 tttaacaatt tctgttgata acgctatttg gaaagttata gacttccaac atgtaaagcc
     1201 tggtaaaggt tcagcattcg ttcgttcaaa attacgtaat ttaagaactg gtgcaattca
     1261 agagaaaacg tttagagctg gtgaaaaagt tgaaccagca atgattgaaa atcgtcgcat
     1321 gcaatattta tatgctgacg gagataatca tgtatttatg gataatgaaa gctttgaaca
     1381 aacagaactt tcaagtgatt acttaaaaga agaattgaat tacttaaaag aaggtatgga
     1441 agtacaaatt caaacatacg aaggtgaaac tatcggtgtt gaattaccta aaactgttga
     1501 attaacagta actgaaacag aacctggtat taaaggtgat actgcaactg gtgccactaa
     1561 atcggcaact gttgaaactg gttatacatt aaatgtacct ttatttgtaa acgaaggtga
     1621 cgttttaatt atcaacactg gtgatggaag ctacatttca agaggataat ctctaatttg
     1681 ttaacaaata gcttgtattc actatactga tttaacgtaa gatattctaa ataagtctca
     1741 taaagctatt gcctaaaatg attataggtt atatg
//
LOCUS       DANBWH010000323         1737 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.334, whole genome shotgun sequence.
ACCESSION   DANBWH010000323 DANBWH010000000
VERSION     DANBWH010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.334"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            162..1385
                     /locus_tag="PPY92_002716"
     CDS             162..1385
                     /locus_tag="PPY92_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HDJ2626650.1"
                     /translation="MTTFSEKEKIQLLADIVELQTENNNEIDVCNYLKDLFDKYDIKS
                     EILKVNEHRANIVAEIGNGSPILALSGHMDVVDAGNQDNWTYPPFQLTEKAGKLYGRG
                     TTDMKGGLMALVITLIELKEQNQLPQGTIRLLATAGEEKEQEGAKLLADKGYLDDVDG
                     LIIAEPTGSGIYYAHKGSMSCKVTATGKAVHSSVPFIGDNAIDTLLEFYNQFKEKYSE
                     LKKHDTKHELDVAPMFKSLIGKEISEEDANYASGLTAVCSIINGGKQFNSVPDEASLE
                     FNVRPVPEYDNDFIESFFQNIINDVDSNKLSLDIPSNHRPVTSDKNSKLITTIKDVAS
                     SYVEQDEIFVSALVGATDASSFLGDNKDNVDLAIFGPGNPLMAHQIDEYIEKDMYLKY
                     IDIFKEASIQYLKEK"
BASE COUNT          603 a          275 c          317 g          542 t
ORIGIN      
        1 agcatacccg atatcagtgt taataagatc gttgatacta gacagttaat ttcatagaac
       61 gaaatcaaat aacacgctac tttctgcatt ttaaattatg tttaagaata aaaattatgt
      121 ttatataaat atatatacta ctttgaaaag gtgtgagctt aatgacaact tttagtgaaa
      181 aagaaaaaat tcaattacta gcagatattg ttgaactaca aactgaaaat aataatgaaa
      241 tagacgtttg taattattta aaagatttat tcgacaagta cgatattaaa tctgaaattt
      301 tgaaagttaa tgaacaccgc gccaatatcg ttgcagaaat cggtaacggc tcacctatac
      361 tcgcattgag tggtcatatg gatgttgttg atgcaggaaa tcaagataat tggacttatc
      421 ccccttttca actcacagaa aaagctggca aattatacgg tcgaggcaca acggatatga
      481 aaggcggtct gatggctttg gtcatcacac tcattgaatt aaaagaacaa aatcaattgc
      541 ctcaaggaac gataagatta ctagctactg ctggcgaaga gaaagaacaa gaaggtgcca
      601 aattattggc tgataaaggc tatttagacg atgtagatgg cttaattatt gctgaaccaa
      661 ctggatctgg aatttattat gcacataaag ggtctatgtc atgtaaagta actgcaactg
      721 gcaaagctgt ccatagctca gttccattta ttggtgacaa tgcaattgat acactgcttg
      781 aattttataa tcaatttaaa gaaaaatatt cagagcttaa aaaacatgat actaaacatg
      841 aattagatgt tgcacccatg ttcaaatcat tgataggaaa agaaatttct gaagaggatg
      901 caaattatgc atctggtctt acagctgtat gttcgattat aaatggcggc aaacaattta
      961 actctgtacc agatgaagct tcacttgaat ttaacgtaag accagttcct gagtatgata
     1021 acgactttat agaatcgttt ttccaaaata tcattaatga tgtggatagc aataagcttt
     1081 cactcgatat tccaagcaat caccgacctg taacaagcga taaaaatagc aaattaatta
     1141 ctacgattaa agatgtagct tctagttatg tagaacaaga cgaaatattt gtttcagcgc
     1201 ttgtaggtgc aacagatgcc tctagtttct taggagataa taaggacaat gttgatttag
     1261 ccatttttgg accaggtaat ccattaatgg cacatcaaat cgatgaatat attgaaaaag
     1321 atatgtacct gaaatatatt gatattttta aagaggcttc cattcaatat ttaaaagaga
     1381 aataagaacg atgctgtcag ctgccctatt cgcgtgctgg cagtttttta ttgcgaattt
     1441 taagttgata gaaatagcga tgaatattgt ttgactgtaa atattcaaat atcaacaagc
     1501 acattttcat tgattaagtg atgtaaaact gaaattatgg tggtgatttg tcatacatat
     1561 attgcattgt ctattgaaat tgggaatcca atttctcttt gttgaggccc ccaccccaac
     1621 ttacattgtt tgtagaattt cttttcgaaa ttctctgtgt tggggccccc accccaactt
     1681 gcattgtctg ttgaaattgg gaatccaatt tctctgtgtt ggggcccctg actagaa
//
LOCUS       DANBWH010000324         1734 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.335, whole genome shotgun sequence.
ACCESSION   DANBWH010000324 DANBWH010000000
VERSION     DANBWH010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.335"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            138..326
                     /locus_tag="PPY92_002717"
     CDS             138..326
                     /locus_tag="PPY92_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626651.1"
                     /translation="MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVI
                     LYIFGFAILIYTFIFGVL"
     gene            514..1083
                     /locus_tag="PPY92_002718"
     CDS             514..1083
                     /locus_tag="PPY92_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626652.1"
                     /translation="MNYIKRTIILLILFVVVSPINSPKTIADNKYSEIQDDKFQLQPG
                     DIIVTKGPVMWGFFGHCSIAIDDKTILQIEGPGDKPTTQSFESFKYNYASGKNDWMKV
                     YRCSYPGAGKKAAEWVKKNYENTNHRYLVTLNLNSKKFTYCTKIIYQAYKFGVSEKSV
                     KSYGLHIISPYAIKDNFIDPYKLRLVKAY"
     gene            1171..1455
                     /locus_tag="PPY92_002719"
     CDS             1171..1455
                     /locus_tag="PPY92_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626653.1"
                     /translation="MNELTKEQKYTIAKFYKLYIERSNNGETETVANFFGDAKDAREN
                     YFCDRDYQDFLTNCQILIQNKYLTGEILDDNIYNISILNKTFIEFEQNFG"
     gene            1517..>1734
                     /locus_tag="PPY92_002720"
     CDS             1517..>1734
                     /locus_tag="PPY92_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626654.1"
                     /translation="MSEWHINNQSTKPFLIQQAEKQLSIVKPLPNGSFQILTEINLEN
                     GNISNIDHSLLVSVDPKALEISIFDAVN"
BASE COUNT          628 a          251 c          248 g          607 t
ORIGIN      
        1 taccattgca atgataacta aaaactggtt aagtgaaata ttatttttta taaatatcgt
       61 actcgttctc tattacattt atcctatttt ttatagtttt ataggttaat aattacggta
      121 tataaggagg gtttcacatg tctttacatt ttgcaattct gttttggcta gcattaattt
      181 tcttagttgc cgctacgttt atactcgtat taatgaaaaa aactggcaaa gaatctaaaa
      241 aagagtccta tttaagtttc actgtcattc tctatatttt tggattcgct atattaatat
      301 acacatttat atttggtgtg ctataaatga aattcatttt atatccagtc tgaaacgtct
      361 caatgatgtt tcagactgtt ttttattctt aatagacatt tcacactctc atactttaat
      421 atttaagata tgttttagtc ttacaagctt attaaagatt acaattctaa tttaagacta
      481 tttacatata tttcttcaac aaggagcttt taagtgaact atattaaacg aacgataatt
      541 ttacttatat tattcgttgt cgtatcccct ataaactcac caaaaacaat tgcagataat
      601 aagtattcag aaattcaaga tgacaaattc caattacaac ctggtgatat tattgttaca
      661 aaaggccctg tcatgtgggg attttttggt cattgtagta tcgcgattga tgataaaacg
      721 attttacaaa ttgaggggcc tggcgacaaa ccaactacac aatccttcga atcttttaaa
      781 tataattatg caagtggcaa aaatgattgg atgaaagttt atcgttgcag ctatcctggt
      841 gcaggtaaaa aagcggctga atgggttaag aaaaactatg aaaatacaaa tcatcgttat
      901 ctagttacat taaatttgaa cagcaaaaaa tttacgtatt gtactaaaat tatctaccaa
      961 gcttataaat ttggtgtaag tgaaaaatca gttaaaagtt atggactaca tattatttca
     1021 ccctatgcaa ttaaagataa ttttatagat ccatacaaac taaggcttgt aaaagcttat
     1081 taacacagtt tcatcaaata gttaaattag tttgtgtttt gcaataaatt gggtatagat
     1141 tacaaatgat atttcaggag gcccaatatt ttgaatgaac taactaaaga acaaaagtat
     1201 acaatagcta aattttacaa attatatatt gaacgttcga ataatggaga aacagaaaca
     1261 gtcgctaact tttttggcga tgctaaagat gcacgtgaaa actatttttg cgatcgtgat
     1321 tatcaagact tcttaactaa ctgccaaatt ttaattcaaa acaaatactt aactggtgaa
     1381 attttggacg ataacattta caatatttct attttaaaca agacatttat tgaatttgaa
     1441 caaaattttg gttgaacatg aatgtctcaa ctataaactt tttaacccta ttttaaagca
     1501 ggagtgaaaa acatacatga gtgaatggca tattaataat caatctacaa aacctttttt
     1561 aatacaacaa gcggaaaagc aattgtctat tgttaaacca ttgcctaatg gtagcttcca
     1621 aatactcaca gaaataaatt tggagaatgg caatattagt aacatcgatc attctttact
     1681 tgtttcagta gaccccaaag cattagaaat aagtatattt gacgctgtaa atta
//
LOCUS       DANBWH010000325         1716 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.336, whole genome shotgun sequence.
ACCESSION   DANBWH010000325 DANBWH010000000
VERSION     DANBWH010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.336"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(364..641)
                     /locus_tag="PPY92_002721"
                     /pseudo
     CDS             complement(364..641)
                     /locus_tag="PPY92_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002464995.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
     gene            complement(783..1697)
                     /locus_tag="PPY92_002722"
     CDS             complement(783..1697)
                     /locus_tag="PPY92_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDJ2626655.1"
                     /translation="MEVPSSAKRDLENENYYNIINGYKDLFLELDVNGNFLVPDKYKQ
                     GTHFKEVFSLYKLDRKFRNVLLEYLLVFETHIKSRISYYFSEKYREPHSYLYFKNYSS
                     DTSKTDSIVKMVATFSSVMSNRKNKPLKHYINTHNGVPLWILVNYLTLGNVSKMYSNL
                     DDDLRLEVAKDYKRKLERDYKTRVQITPSDVDSILQQAHMFRNVCAHEERLYDYKIDR
                     AKSRANIFANYNKIYDKEYVPTMNGSYVFDLLISLCLFLNKHDYIKLMKNMDKLISNY
                     SHSFYTITIDDLYTKMNFPDQTKILDML"
BASE COUNT          557 a          309 c          211 g          639 t
ORIGIN      
        1 actagctata tattatacaa ctactataac aactcaatat taaatgcctt atgtgttaat
       61 tgtctaattt gctataaaac ttagtaagtc atatagtcga ttcctatcgt atagagatat
      121 aataataggc aagtaccgaa gtacctgccc agaaaaggat cattcatttt tttactctaa
      181 ctgatttctc cacataagtc gcttaataac tgactagttg gaacagaacc attccatgtt
      241 ctaatatgca agtaataacg ttgcccctcc cgtttatata atacccataa ataacattta
      301 cataactaat atatgcaatt ttacaagact caaaattgag ttctgccgta aaatcataaa
      361 catttagtat ggaattttag tttcaccatt tgatgttatg tgttctttgc tgcgttctgg
      421 ctcaggaaga gttgcgattt gatacaaaac atttaaccct aaatttaacg acgtcgttaa
      481 atttgaacct tcgtactctt cactaacttt cataaatcta tgcgcttgtt ggttgcttag
      541 cttcactttt ttagccactt accaaactct ccgtgcgcta agtcattttc tttcacatgc
      601 ttcaatcttc ggccagtctc gaatatcgac tgaccagcga tgtttgaagt cgtatttcac
      661 gacgtcacac ttacgattag catatctagt tacagaatct tatcccccac tgcaacacag
      721 ggcgtttctc agcgtcttaa aataaaaaaa cgccactcgt aagtgacatt aaaaaatatc
      781 ttttataaca tatccagtat ttttgtttga tctggaaaat tcatttttgt atataggtca
      841 tctatagtaa ttgtatagaa agaatgtgaa taattactta ttagtttatc catatttttc
      901 atcaatttta tgtaatcatg tttattcaaa aatagacata gtgaaatcaa caaatcgaat
      961 acataactac cattcattgt aggaacgtat tctttatcgt atattttgtt ataattggcg
     1021 aatatattag ctctactttt agccctgtct attttataat catacaatct ttcttcatgc
     1081 gcacacacgt tacgaaacat atgtgcttgt tgtagtatac tgtctacatc tgatggagtt
     1141 atttgaacac gtgttttata atctctttcc aatttccttt tatagtcttt agcaacttcc
     1201 aatcgaagat catcatccaa attggaatac atttttgaaa cattacctaa agttaaataa
     1261 ttcaccaata tccatagtgg cactccatta tgagtattaa tataatgttt taatggttta
     1321 ttttttctat tactcataac cgagctaaat gtagcaacca ttttcacgat gctatctgtc
     1381 ttacttgtgt cagatgaata atttttaaag tataaatatg aatgtggttc tctatatttt
     1441 tcgctaaaat aatatgaaat tcttgattta atatgagttt caaatactaa caaatactct
     1501 aataaaacat tcctaaattt tctatctagt ttgtataaag aaaagacttc tttaaaatga
     1561 gtaccttgct tatatttatc aggaaccaaa aaattaccat taacatctag ttctaaaaat
     1621 aaatctttat aaccatttat gatattataa taattttcat tttctaaatc tcttttagca
     1681 ctactcggta cttccattcc tcttcttctt agaatt
//
LOCUS       DANBWH010000326         1688 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.337, whole genome shotgun sequence.
ACCESSION   DANBWH010000326 DANBWH010000000
VERSION     DANBWH010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.337"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..458)
                     /locus_tag="PPY92_002723"
     CDS             complement(<1..458)
                     /locus_tag="PPY92_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /protein_id="HDJ2626656.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHH
                     STVYRWVQEYAPVLYQIWKKKHKKAYYKWRVDETYIKIKGQWCYLYRAIDADGHTLDI
                     WLRKQRDNHSAYAFIKRLIKQFGKPQKVITDQAPSTKVAMAKVIKAFKLKP"
     gene            complement(729..824)
                     /locus_tag="PPY92_002724"
     CDS             complement(729..824)
                     /locus_tag="PPY92_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDJ2626657.1"
                     /translation="MLEILVHITTTVISGCIIALFTHWLRNRNNK"
     gene            989..1654
                     /locus_tag="PPY92_002725"
     CDS             989..1654
                     /locus_tag="PPY92_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001568510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257-2 family transposase"
                     /protein_id="HDJ2626658.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHH
                     STVYRWVQEYAPVLYQIWKKKHKKAYYKWRVDETYIKIKGQWCYLYRAIDADGHTLDI
                     WLRKKRDHQSAYAFIKRLIKQFGKPQMIITDQAPSTKVAMAKIIKVFKLKPNCHCTSK
                     YLNNFIEQDHRHIKVRKTRYQSVNTAKNTLKGIECIYGLYKKTVGLFRSTDFHHATKL
                     VIC"
BASE COUNT          566 a          295 c          301 g          526 t
ORIGIN      
        1 ggtttaagtt taaaagcttt aattacttta gccattgcta ccttcgttga aggtgcctga
       61 tctgtaatta ccttttgagg tttaccaaat tgtttaatga gacgtttgat aaacgcatat
      121 gctgaatgat tatctcgttg cttacgcaac caaatatcta atgtatgtcc atctgcatca
      181 atcgcgcgat acagataaca ccactgtcct ttaattttga tatatgtctc atcaacacgc
      241 cacttataat acgctttttt atgttttttc ttccaaattt gatacaaaac gggagcatat
      301 tcttgaaccc aacggtagac cgttgaatga tgaacgttta caccacgttc ccttaatatt
      361 tcagatatat cacgataact caatgcatat cttagatagt agccaaccgc tacagtgata
      421 acatccttgt taaattgttt atatctgaaa tagttcatac agaagactcc tttttgttaa
      481 aattatacta taaattcaac tttgcaacag aaccagttaa acaatgttat gccaagttac
      541 aatgttttta tccgtaaaga cgagttagat tatagataga aataatattc aaaagagaaa
      601 tgaatgagga ctttgcattt ctccactcac cgatgttata atttagttaa caaggaaact
      661 agcatagatg caccaatccc ctcactacgg caatagtgag gggatttttt aggtgtggct
      721 atggtcgcct atttattatt gcgattacgt agccaatgcg taaataacgc aataatacaa
      781 ccactgatga ctgtggtcgt gatgtgaaca aggatttcta gcatagatta gcacctcctc
      841 tctgcgtcgg aattgacgcc tgagagatag acgacttgta tattatatca tctacgaata
      901 tacgatgata ggtagcttct gataagtgat ctggttctgt tgcaaagttg attttgtagt
      961 ataattttaa caaaaaggag tatgctatat gaactatttc agatataaac aatttaacaa
     1021 ggacgtcatc actgtagccg ttggctacta tctaagatat gcgcttagtt atcgtgatat
     1081 atctgaaata ctaagagaac gtggtgtcaa cgttcatcat tcaacggtct accgttgggt
     1141 tcaagaatat gctcccgttt tgtatcaaat ttggaagaaa aaacataaaa aagcgtatta
     1201 taagtggcgt gttgatgaga catatatcaa aattaaagga cagtggtgtt atctgtatcg
     1261 cgcgattgat gcagatggac atacattaga tatttggtta cgcaaaaaac gagatcatca
     1321 atcagcatat gcgtttatta agcgccttat taaacaattc ggtaaacctc aaatgataat
     1381 tacagatcaa gcaccttcaa cgaaggtagc catggctaaa ataattaaag tgtttaagtt
     1441 gaaacctaac tgtcattgca catctaaata tctcaataac ttcattgaac aagatcatcg
     1501 tcacattaag gtgagaaaga caagatatca aagtgtcaat acggcaaaaa atacactcaa
     1561 aggcattgaa tgtatttacg gattatataa aaaaaccgta ggtctcttca gatctacgga
     1621 ttttcaccat gccacgaaat tagttatatg ttagctggtt aggcgaagac tgacacaata
     1681 aaatggtg
//
LOCUS       DANBWH010000327         1677 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.338, whole genome shotgun sequence.
ACCESSION   DANBWH010000327 DANBWH010000000
VERSION     DANBWH010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.338"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(154..1215)
                     /locus_tag="PPY92_002726"
     CDS             complement(154..1215)
                     /locus_tag="PPY92_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HDJ2626659.1"
                     /translation="MSNANNADNKQFFSKILNSIGAGVVIALVPNALLGEVLKIFKSG
                     NEILELTYQLVILIQLFMAFIIGVLAAHQFKFNGAGAAIVGTSAMIGSGAVVYSNNSF
                     MLKGIGDIINTSLVVIIACLIYMVLQNKLGSFELIILPVLVPIVSGGIGLITLPYIRK
                     ITQAIGNVIHSFTDLNPLLMSILISVAFSLLMVTPISLVAIATAISLNGLGSGAANLG
                     IVAACVTFLFGSLRVNSIGVNAVLLIGAAKMMIPVYLKNLIISIPLTINGIITGIIAY
                     VLQVKGTPLSAGFGYTGLVGPINAFNRMSGDPTMNIILLALGYFVIPFVSAFIVHELC
                     KKFIPIYSNDIYKFEVPKQ"
BASE COUNT          663 a          289 c          195 g          530 t
ORIGIN      
        1 ttttaaaaga ctgtacataa aaaatccgct attatacgtg aacaaatctc acattttaaa
       61 atcaatcaaa aaagcgctac tatcaatatc ttattgacag taacgcctat atttatcata
      121 tattaaaact tattcttgtc atcaagctat caattattgt tttggaactt caaatttata
      181 aatatcatta gaatatatag gaatgaattt tttacacaat tcatgaacaa taaatgccga
      241 tacaaatggt atgacaaaat aaccaagtgc taataaaata atattcattg ttggatcacc
      301 tgacattcgg ttaaatgcat ttattgggcc aactaatcct gtatatccaa atccagcaga
      361 caatggtgtt cctttaacct gaagaacgta agcaatgatt ccagtaatta ttccatttat
      421 agtcaatggt atcgaaataa ttaaattttt caagtaaact gggatcatca tttttgcagc
      481 tcctatgagt aaaactgcat ttacaccaat agaattgaca cgcaatgagc caaataagaa
      541 agtaacacag gcagccacta tacctaggtt tgctgctcca cttcctagtc cgtttaaact
      601 aatcgcagtt gcaatcgcta ctaacgatat tggtgttacc attaataatg aaaatgccac
      661 actaataagt atagacatta acaacggatt taagtctgta aaagaatgaa ttacatttcc
      721 aattgcttga gtaatttttc gaatgtaagg taatgtgatt agaccgatac ccccactaac
      781 gataggtact aaaactggta atataattaa ttcaaaagat ccaagtttgt tttgtaatac
      841 catatatata agacatgcaa taataacaac caaactcgta tttataatgt cacctatacc
      901 ttttaacata aaactattat tgctatatac aacagcacct gaaccaatca tagctgatgt
      961 acctactata gcagcacctg cgccattaaa tttaaattga tgagcagcta aaaccccaat
     1021 aataaatgcc ataaataatt gaattagtat cactaactga tacgttaatt ctaaaatttc
     1081 attaccactt ttaaatattt ttaatacttc acctaataaa gcattcggaa caagtgcaat
     1141 aacaacacca gcaccaatag aatttaaaat cttactgaaa aactgtttat tatcagcatt
     1201 atttgcgtta ctcataaacg acctccaatt tgaacattaa actcatcata ttatagaaaa
     1261 ttaactttaa caatgattag ttggctaatt taatctaaat ataacaatcc acaaaatgaa
     1321 taaagtaaat ttaaacaatc tactcactaa tcaatgtcat gctaatctta aaatactact
     1381 aattacatcc tgtgactaat aatgtaataa attgtaaata ttaaaatagc catattacta
     1441 ttaagataga taaattgaat cgacttggta taaagcacaa taaaatagca cttttacaaa
     1501 tatattatta gaaaagttga aacaaatata taaccccaga taatataatc attatcatta
     1561 atgtaatacc aaaaaaagaa gttaaacaat gcatagttgc ttaacttcca atattgaact
     1621 catcattaca atttgacata gagtctatta aagcgtgtgc catttgagtc cactttt
//
LOCUS       DANBWH010000328         1673 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.339, whole genome shotgun sequence.
ACCESSION   DANBWH010000328 DANBWH010000000
VERSION     DANBWH010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.339"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            56..595
                     /locus_tag="PPY92_002727"
     CDS             56..595
                     /locus_tag="PPY92_002727"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDJ2626660.1"
                     /translation="MTKNEIRKYILHKMKNFNKAEKRKADTWLRNQFFATEEYKEANA
                     IALVLSFNHEVDTFSIIEQALMDHKRIFVPKMDYLNHQMTFKEIFNLKDIDVDNKGIY
                     YPTSKGETTNNLDLIVVPGVGFQDDGYRIGYGGGYYDRFLANYQTKTISLLYDFQITS
                     FEPESFDQPVDKLIIYQSA"
     gene            607..>1673
                     /locus_tag="PPY92_002728"
     CDS             607..>1673
                     /locus_tag="PPY92_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDJ2626661.1"
                     /translation="MNIDKQFWKTIYYWIRYLNFDIVSREKDDQEIWLAHKRKKQVVI
                     FKQHIKSTQEIRFDKAKVLEHKDEIANFISFEPQSFEFYYFTESEFSEEQLNEVSPIR
                     IKFNVIRHTKDLIKHMPNIFLARLISEDNDKKTYMFYKRKVLTDNFLDKYMQKFSPAT
                     YTIIFVNVLIWLCMILYLNNFSDVKLLDVGGLVHFNVVHGEWYRIVTSMFLHFSFEHI
                     LMNMLSLFIFGKIVEAIIGSWRMLTVYFIAGLFGNFVSLSFNTTTISVGASGAIFGLI
                     GSIFAMMYVSKTFNKKMLGQLLIALVILVGVSLFMSNINIVAHIGGFIGGLLITLIGY
                     YYKVNRNIFWILLIGMLVIFIA"
BASE COUNT          606 a          190 c          287 g          590 t
ORIGIN      
        1 aatgacatgt attgttatta acgatataat agaattgaat aaagtgggtg ataatgtgac
       61 taaaaatgag attagaaaat acattttaca taaaatgaag aattttaata aagctgaaaa
      121 gcgaaaagca gacacatggt taagaaatca attttttgca actgaagaat acaaagaagc
      181 aaacgcaatt gcgctagttc tttcttttaa tcatgaagta gatacttttt ctattattga
      241 acaagcctta atggatcata aacgtatttt tgtaccgaaa atggattatt taaatcatca
      301 aatgactttt aaagaaatat ttaatctcaa agatattgat gtcgataata aggggattta
      361 ctatccaact tcaaaaggtg aaacaacgaa taacctagat ttaattgttg ttcctggtgt
      421 tggatttcaa gacgatggat atagaattgg gtatggtggt ggctattacg acaggttttt
      481 agctaattat cagacaaaga caataagctt attatacgat tttcaaataa catcatttga
      541 accggaatca ttcgatcaac cagtcgataa attgattata tatcaatcag catagtggag
      601 gactaaatga acatagacaa acaattttgg aaaacaatat attattggat taggtacttg
      661 aattttgata ttgtcagtag ggaaaaagat gaccaagaaa tttggttagc ccataaaaga
      721 aaaaaacaag tagtcatttt taaacaacat atcaagtcta cccaagaaat acgttttgac
      781 aaagcgaaag tgcttgaaca caaagatgaa atagcaaatt ttatttcttt cgaaccacaa
      841 agttttgaat tttattattt tacagaatcg gaattttcag aagaacaatt aaatgaagtt
      901 tcgccaatta gaattaaatt caatgttata agacacacaa aagatttgat aaagcatatg
      961 ccgaatatat ttttggctag acttatttca gaagataatg ataaaaagac atatatgttc
     1021 tataaacgca aagtattaac cgataacttt ttagataaat atatgcagaa attttcaccg
     1081 gcaacataca caataatatt tgtaaatgtc ttaatatggt tatgtatgat tttatattta
     1141 aataattttt cggatgtaaa attattagat gttggcgggt tagtgcattt taatgtcgta
     1201 catggtgaat ggtatcgaat tgttacatcg atgtttttac attttagttt tgaacatata
     1261 cttatgaata tgctttcatt atttattttt ggtaaaatag tcgaagcaat tattggttca
     1321 tggcggatgt taactgtata ctttattgca gggttgtttg gaaactttgt atcactatca
     1381 tttaatacga ctacaatttc agttggggct agtggtgcta tatttggtct gattggatca
     1441 atttttgcga tgatgtatgt ttcaaaaaca tttaacaaaa aaatgttagg acagttatta
     1501 attgcattag tgatattagt tggtgtttct ctgtttatgt caaatataaa tattgtggcg
     1561 catattggag gattcattgg tggtttatta ataactttaa ttggctatta ctataaagtg
     1621 aatcgtaata ttttttggat tttactaatt ggtatgcttg ttatatttat tgc
//
LOCUS       DANBWH010000329         1672 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.340, whole genome shotgun sequence.
ACCESSION   DANBWH010000329 DANBWH010000000
VERSION     DANBWH010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.340"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(99..1580)
                     /gene="cls"
                     /locus_tag="PPY92_002729"
     CDS             complement(99..1580)
                     /gene="cls"
                     /locus_tag="PPY92_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDJ2626662.1"
                     /translation="MRYTFSNDLGTLFTIILAIGFIINLVLAFIIIFLERNRRTASST
                     WAWLFVLFVLPLIGFILYLFFGRTVSARKLNKNNGNVLTDFDGLLKQQIESFDKGNYG
                     TDNKQVQKHHDLVSMLLMDQDGFLTENNKVDHFIDGNDLYDQVLKDIKNAKEYIHLEY
                     YTFALDGLGKRILHALEEKLKQGLEVKILYDDVGSKNVKMANFDHFKSLGGEVEAFFA
                     SKLPLLNFRMNNRNHRKIIVIDGQLGYVGGFNIGDEYLGLGKLGYWRDTHLRIQGDAV
                     DALQLRFILDWNSQAHRPQFEYDVKYFPKKNGPLGNSPIQIAASGPASDWHQIEYGYT
                     KMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSGVDVHLMIPCKPDHPLVYWATFSNA
                     SDLLSSGVKIYTYENGFIHSKMCLIDDEIVSVGTANMDFRSFELNFEVNAFVYDENLA
                     KDLRVAYEHDITKSKQLTKESYANRPLSVKFKESLAKLVSPIL"
BASE COUNT          578 a          300 c          216 g          578 t
ORIGIN      
        1 acaaatgtca ttattaggtt tacataatat atttatcgtt attttaattt tgggcaaaat
       61 aaaaagagcc tctataatcg agactcctta caaataaatt ataaaattgg cgaaactaat
      121 tttgctaacg attctttgaa tttaacagac agcggtctat tggcatatga ttctttggtt
      181 agttgttttg attttgtaat atcatgttca taagccaccc ttaaatcctt agcaagattt
      241 tcatcatata caaaggcatt tacttcaaaa tttaattcaa aacttctaaa gtccatattt
      301 gctgtgccca ctgatacgat ttcatcatca attaagcaca ttttagaatg tataaatcca
      361 ttttcatacg tataaatttt aacaccactt gataataagt cagaggcatt tgaaaatgtc
      421 gcccaataaa ctaaaggatg atctggctta catggaatca ttagatgaac atctacacct
      481 gatttagctg caattttaat ggcatttata tatgagttat ccggaatgaa ataaggcgat
      541 tgcaaatata ccgacttctt agcgctcata atcatttttg tataaccata ttcaatttga
      601 tgccagtcac tagctgggcc acttgcagct atttgaattg gtgaattgcc caatggtccg
      661 ttctttttag ggaaatactt aacatcatat tcaaactgtg gacggtgcgc ttgcgaattc
      721 cagtctaaaa taaatcgcaa ctgcagtgca tcaaccgcat ccccttgtat acgtaaatgc
      781 gtatctctcc aataccctag tttacctaat cctagatatt catcaccaat gttaaatcct
      841 ccgacataac ctagttgacc atcgattacg atgatttttc tatgatttct gttattcata
      901 cggaaattta ataagggtaa tttagaagca aaaaatgctt caacttcgcc acctaatgat
      961 ttaaagtgat caaaatttgc catcttaaca tttttagatc caacatcatc atataatatt
     1021 tttacttcta gaccttgttt caatttttct tctaaagcat gtaaaattct tttacctaaa
     1081 ccatctaaag cgaaagtata gtactctaaa tggatatatt cttttgcatt tttaatatct
     1141 tttaaaactt gatcatataa atcatttcca tcaatgaaat gatcaacttt attattttca
     1201 gttaaaaaac catcttgatc catcaaaagc atacttacta aatcatgatg tttttgaact
     1261 tgtttgttat cagtaccata attaccttta tcaaagcttt ctatttgttg ttttaaaagt
     1321 ccatcgaaat ccgttaacac gttaccattg tttttattca atttacgtgc cgaaacggtt
     1381 ctaccaaaaa acaagtaaag aataaaacca atcaatggta agacaaaaag tacaaatagc
     1441 catgcccaag ttgaactcgc tgtacgtcta tttctttcta aaaagataat aataaaagcc
     1501 aatactaaat taatgatgaa tccaatggct aaaataatgg taaaaagtgt tcctaaatca
     1561 tttgaaaatg tatatcgcat agtcttttct cctataaaga aaggcacaaa aaacattttg
     1621 cacctttcac atcatattat ttattcacag ataaagttaa aattgcattg aa
//
LOCUS       DANBWH010000330         1624 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.341, whole genome shotgun sequence.
ACCESSION   DANBWH010000330 DANBWH010000000
VERSION     DANBWH010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.341"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            277..1002
                     /locus_tag="PPY92_002730"
     CDS             277..1002
                     /locus_tag="PPY92_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL14"
                     /protein_id="HDJ2626663.1"
                     /translation="MKKNIMNKLVLSTALLLLGTTSTQLPKTPISFSSEAKAYNISEN
                     ETNINELIKYYTQPHFSLSGKWLWQKPNGSIHATLQTWVWYSHIQVFGSESWGNINQL
                     RNKYVDIFGTKDEDTVEGYWTYDETFTGGVTPAATSSDKPYRLFLKYSDKQQTIIGGH
                     EFYKGNKPVLTLKELDFRIRQTLIKNKKLYNGEFNKGQIKITADGNNYTIDLSKKLKL
                     TDTNRYVKNPKNAQIEVILEKSN"
     gene            1097..>1624
                     /locus_tag="PPY92_002731"
     CDS             1097..>1624
                     /locus_tag="PPY92_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein"
                     /protein_id="HDJ2626664.1"
                     /translation="MNNNITKKIILSTTLLLLGTAFTQFPNTPINSSSEAKAYYINQN
                     ETNVNELTKYYSQKYLTFSNSTLWQKDNGTIHATLLQFSWYSHIQVYGPESWGNINQL
                     RNKSVDIFGIKDQETIDSFALSQETFTGGVTPAATSNDKHYKLNVTYKDKAETFTGGF
                     PVYEGNKPVLTLKELD"
BASE COUNT          627 a          249 c          235 g          513 t
ORIGIN      
        1 taaagtataa ctatttttca agtagttaga aaattcaatt atcaaacaat ttaatgcaat
       61 tgattagaga ataattgtaa cgtgtcgttt ttaatatata actcccgcct actttattaa
      121 gtactgtttc tgtccaaaac ttaaaaatga taagttttgc ttaaataaca ctactaactg
      181 tttaagttta tttaacatag ttttagcttt tatttaattc cgaatcggtg taatagctta
      241 tatactttgg gtaattcacg caaaggagat tttcatatga aaaagaacat catgaataaa
      301 ttagttttat caacagcatt gttactttta ggaaccacat caacacaact tcctaaaaca
      361 ccaatcagtt tttcatctga agcaaaagcc tataatatca gtgaaaacga gactaatatc
      421 aatgagttaa tcaaatatta cactcagccg catttttcat tatctggaaa atggctatgg
      481 caaaagccca atggtagcat tcatgcaaca ttgcaaacgt gggtttggta tagtcatatt
      541 caagtgtttg gatccgagag ttggggaaac attaatcagt taagaaataa atacgttgat
      601 atatttggaa ctaaagatga ggacacagtt gaaggttact ggacttatga tgaaacattt
      661 actggtggtg ttacgccagc agctacttca tctgataaac cttatagact atttttaaaa
      721 tatagtgata aacaacaaac tatcatcggt ggacatgaat tttacaaagg aaataaacca
      781 gtattaactt taaaagaatt agatttccgt attcgtcaaa cattaataaa gaataaaaag
      841 ttatataacg gagaatttaa taaaggtcaa attaagataa ctgctgatgg aaataattac
      901 acgattgatt taagtaaaaa gttaaaatta actgacacaa accgttatgt taaaaatcct
      961 aaaaatgcac aaattgaagt catactcgaa aaatctaact aacctattac cttttgtaaa
     1021 tgcggataat ttcaattatc taattaaccc ctttttataa ttaaacattc caacaatact
     1081 caaaggagaa attcgaatga acaataacat cacgaaaaaa attattttat caacaacatt
     1141 gttactatta ggtacagcat ttacacaatt tcctaataca cctatcaatt cttcatctga
     1201 agcgaaagct tattatataa atcaaaacga aactaacgtt aatgagttaa ctaaatatta
     1261 ctcgcaaaaa tatttaacct tctctaacag tacgttatgg caaaaagata acggtacgat
     1321 tcatgcaacg ttgttacagt tttcttggta tagtcatatt caagtttatg gacctgaaag
     1381 ttggggcaat atcaaccaat taagaaataa aagcgttgat atttttggca taaaagacca
     1441 agaaaccatt gattcttttg cattatctca agaaacgttt actggtggtg ttactcctgc
     1501 agcaacatct aacgataaac actataaact gaatgtaaca tataaagata aagcagaaac
     1561 gtttactggc ggatttccag tttatgaagg caataagcct gttttaactt taaaagaatt
     1621 agat
//
LOCUS       DANBWH010000331         1616 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.342, whole genome shotgun sequence.
ACCESSION   DANBWH010000331 DANBWH010000000
VERSION     DANBWH010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.342"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(299..372)
                     /locus_tag="PPY92_002732"
     tRNA            complement(299..372)
                     /locus_tag="PPY92_002732"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(336..338),aa:Arg,seq:tct)
     gene            736..870
                     /locus_tag="PPY92_002733"
     CDS             736..870
                     /locus_tag="PPY92_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HDJ2626665.1"
                     /translation="MEGLFNAIKDTVTAAINNDGAKLGTSIVSIVENGVGLLGKLFGF
                     "
     gene            927..1061
                     /locus_tag="PPY92_002734"
     CDS             927..1061
                     /locus_tag="PPY92_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HDJ2626666.1"
                     /translation="MTGLAEAIANTVQAAQQHDSVKLGTSIVDIVANGVGLLGKLFGF
                     "
     gene            1185..>1616
                     /locus_tag="PPY92_002735"
     CDS             1185..>1616
                     /locus_tag="PPY92_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HDJ2626667.1"
                     /translation="MGKLGYKNILIDFDDTIVDFYDAEEWAFHYMANVFNHKATKDDF
                     LTFKKINHQHWEAFQQNKLTKSEVLSERFVNYFKHHQMEVDGHRADVLFRNGLAEAKV
                     KYFDQTLETIVELSKRHDLYIVTNGVTETQKRRLNQTPLHKY"
BASE COUNT          580 a          215 c          303 g          518 t
ORIGIN      
        1 aatggtgagt gttaaaaatc ttggtgtagt gttggtgtag ttcaggtgca gtatagatga
       61 aatttgagca caaaattact taattaaaaa tgtataaaca agtgtcagac gcgtgtcaat
      121 tgtgtgtcaa aaaattctat gacgaataag catgcttgtt ttaattactg tattttcggg
      181 gcttatgcgg gggacgtttt ggtgacgcat catactatat tactgaaatt caaaacaaaa
      241 agagccccgt aatcacggaa ctcttttgtt tggtaatgcg tataaaaata cctataaacg
      301 tcctgggagg gattcgaacc cccgaccgat ggcttagaag gccattgctc tatccagctg
      361 agctaccagg acacgtttaa caacacaaga attattatat ctaaatgaac ttaaattagc
      421 aataccttgt aaataaaaaa tgtttatatt tttcactatt atagagctat ttatctaaaa
      481 aggttcaata agacttaaat gcgaattcag gcaacttaat tgtgttaaat acagttttga
      541 atgcctaact gtatttcttt tctctttaaa atacagttaa gtacattata agatgttgtg
      601 cggataaaca aactaattgc atcaaattta ttttaaaata acaacaacaa aacgttaagc
      661 gaataacatt tcggtgattt aaaagctacg cacgtttttg ttatcttcaa atttaaattt
      721 taaggagtgt tttcaatgga aggtttattt aacgcaatta aagataccgt aactgcagca
      781 attaataatg atggcgcaaa attaggcaca agcattgtga gcatcgttga aaatggcgta
      841 ggtttattag gtaaattatt cggattctaa tttcaatatg ttatgtaagt aatcagtatt
      901 atttcaaagg tgagggagag atttaaatga ctggactagc agaagcaatc gcaaatactg
      961 tgcaagctgc acaacaacat gatagtgtga aattaggcac aagtatcgta gacatcgttg
     1021 ctaacggtgt gggtttacta ggtaaattat ttggattcta atataataac taatattctt
     1081 taaaataaac tgggtgagca tactttaatg ttatgcactc agtttatttt atttgcagaa
     1141 atttgagcct ctgttaagat ttagatacat agacaatata ggagatgggg aaattgggat
     1201 ataaaaatat tttgatagac tttgatgata caattgttga tttttatgat gcagaagaat
     1261 gggcgtttca ctatatggcg aatgttttta atcataaagc aacaaaggat gattttttaa
     1321 catttaaaaa aatcaatcac caacattggg aagcttttca acaaaataaa ttaacgaagt
     1381 ctgaagtatt atcagaacga tttgtgaatt acttcaaaca tcatcaaatg gaagttgatg
     1441 ggcatcgtgc agatgtgtta tttagaaatg gattagcaga agctaaagtt aaatactttg
     1501 atcaaacatt agaaacaatt gtcgaattat cgaaaagaca tgatttatat attgttacta
     1561 atggtgtaac cgaaacgcaa aagagaaggt taaatcagac gccgttgcat aaatat
//
LOCUS       DANBWH010000332         1596 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.343, whole genome shotgun sequence.
ACCESSION   DANBWH010000332 DANBWH010000000
VERSION     DANBWH010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.343"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(38..946)
                     /gene="rocF"
                     /locus_tag="PPY92_002736"
     CDS             complement(38..946)
                     /gene="rocF"
                     /locus_tag="PPY92_002736"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HDJ2626668.1"
                     /translation="MTKTKAIDIIGAPSTFGQRKLGVDLGPTAIRYAGLISRLKQLDL
                     DVYDKGDIKVPAVNIEKFHSEQKGLRNYDEIIDVNQKLNKEVSASIENNRFPLVLGGD
                     HSIAVGSVSAISKHYNNLGVIWYDAHGDLNIPEESPSGNIHGMPLRILTGEGPKELLE
                     LNSNVIKPENIVLIGMRDLDKGERQFIKDHNIKTFTMSDIDKLGIKEVIENTIEYLKS
                     RNVDGVHLSLDVDALDPLETPGTGTRVLGGLSYRESHFALELLHQSHLISSMDLVEVN
                     PLIDSNNHTAEQAVSLVGTFFGETLL"
     gene            complement(1085..1157)
                     /locus_tag="PPY92_002737"
     tRNA            complement(1085..1157)
                     /locus_tag="PPY92_002737"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1122..1124),aa:Lys,seq:ttt)
     gene            complement(1161..1235)
                     /locus_tag="PPY92_002738"
     tRNA            complement(1161..1235)
                     /locus_tag="PPY92_002738"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1201..1203),aa:Gln,seq:ttg)
     gene            complement(1245..1328)
                     /locus_tag="PPY92_002739"
     tRNA            complement(1245..1328)
                     /locus_tag="PPY92_002739"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1292..1294),aa:Tyr,seq:gta)
     gene            complement(1358..1433)
                     /locus_tag="PPY92_002740"
     tRNA            complement(1358..1433)
                     /locus_tag="PPY92_002740"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1398..1400),aa:Val,seq:tac)
     gene            complement(1441..1512)
                     /locus_tag="PPY92_002741"
     tRNA            complement(1441..1512)
                     /locus_tag="PPY92_002741"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1477..1479),aa:Glu,seq:ttc)
     gene            complement(1514..1588)
                     /locus_tag="PPY92_002742"
     tRNA            complement(1514..1588)
                     /locus_tag="PPY92_002742"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1554..1556),aa:Asn,seq:gtt)
BASE COUNT          491 a          344 c          255 g          506 t
ORIGIN      
        1 aaagcaaaat atactttata cactacaaat catttattta taataaagtt tcaccaaaaa
       61 atgttccaac taatgaaacc gcttgttcag cagtatgatt attactgtca atcaatggat
      121 ttacttcaac taaatccatt gaggaaatta aatgtgattg atgcagtaat tccaatgcaa
      181 aatggctttc tctataacta agaccaccca aaactctagt accagtgcct ggcgtttcaa
      241 gcggatctaa agcatcaaca tctaaagata aatgaacgcc atcaacattg cgtgacttca
      301 aatattctat tgtattttca attacttcct ttatccctaa tttatcaata tctgacatag
      361 taaatgtttt aatattatga tctttgataa attgtctttc acctttatct aaatctctca
      421 taccaattag tacgatgttt tctggcttga ttacattact atttaattct aaaagttctt
      481 tgggaccttc gcctgtcaaa atccttagag gcataccatg aatatttcca cttggtgact
      541 cttcaggtat atttaaatca ccatgtgcat cataccaaat aacacctaaa ttattataat
      601 gtttacttat tgctgatact gaacctaccg caatagaatg atcaccacca agaactagag
      661 gaaatctgtt attttcaatt gatgctgaaa cctctttatt taatttttga ttaacatcta
      721 taatttcatc ataatttctt aatccttttt gttcactatg aaatttttca atgttcacag
      781 caggtacctt aatatccccc ttgtcatata catcaaggtc taattgcttt aatcttgaaa
      841 ttaatccagc atatctaatt gctgttggtc ctaaatcaac acctaatttt ctttgtccaa
      901 atgttgatgg tgcacctata atatcaattg cttttgtctt tgtcataagc gtcccccttt
      961 gctctatgta attaaagaat aatgtatgcg cttaccatta tcaagcaata gctacacata
     1021 taatctgttt atcttattac ttcataaaaa aaaagcactt cccaaaaatg gaaagtgcaa
     1081 gtagtgagcc atagaggatt cgaacctctg accctctgat taaaagtcag atgctctacc
     1141 aactgagcta atggctctaa tggctgggct agctggattc gaaccaacga gtgacggagt
     1201 caaagtccgt tgccttaccg cttggctata gcccaatata tagatggtgg aggggggcag
     1261 attcgaactg ccgaacccga aggagcggat ttacagtccg ccgcgtttag ccacttcgct
     1321 acccctccag cttattcata taatttaata atcaaaatgg tggagaatga cgggttcgaa
     1381 ccgccgaccc tctgcttgta aggcagatgc tctcccagct gagctaattc tccaaaataa
     1441 tgactcctac gggactcgaa cccgtgttac cgccgtgaaa gggcggtgtc ttaaccgctt
     1501 gaccaaggag ccatggctcc acaggtagga ctcgaaccta cgaccgatcg gttaacagcc
     1561 gatagctcta ccactgagct actgtggatt aatatt
//
LOCUS       DANBWH010000333         1592 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.344, whole genome shotgun sequence.
ACCESSION   DANBWH010000333 DANBWH010000000
VERSION     DANBWH010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.344"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(325..1416)
                     /locus_tag="PPY92_002743"
     CDS             complement(325..1416)
                     /locus_tag="PPY92_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase"
                     /protein_id="HDJ2626669.1"
                     /translation="MSNKLESYRSEIVSLNHQILDLLSKRGELAQKIGEEKLKQGTRI
                     YDPQREKEMLNDLIDSNKGPFNDNTIKQLFKEIFKASTDLQKSENEKHLYVSRKLKPE
                     DTIVTFDNGGIIGDGNKSFVFGPCSVESFEQVEAVAKNLHAKGEKFIRGGAFKPRTSP
                     YDFQGLGVEGLKILKQIKDKYDLNVVSEIVNPNDFEVADEYLDVFQIGARNMQNFELL
                     KEAGRTKKPILLKRGLSATIEEFVYAAEYIASQGNQNIILCERGIRTYEKATRNTLDI
                     SAVPILKQGTHLPVMVDVTHSTGRKDIMLPTAKAALAVGADGVMAEVHPDPSVALSDA
                     GQQMDLDEFQAFYDELKPLADLYNAKKLK"
BASE COUNT          506 a          280 c          218 g          588 t
ORIGIN      
        1 ttcaaacatc aattgcattt gttgaaaaat gttcacattt cagcaacaaa tgtgtgaatc
       61 tattatattt tacagataat tcatagttat aattttaatt actgctatca gtcataatct
      121 ttaataattg tgaaattgaa caagtgtcga agtattataa caatcgttaa gtgcagtata
      181 cctttatttc tatacaaaaa ataatagcca ctctaaaagt aatatactct tagaatggct
      241 gtaagtttaa aattgtcgat tcaaagataa tattctactt caacatgaca tattagttaa
      301 gtagtctata gtttccttgg aatattattt taacttttta gcgttatata aatcagctaa
      361 aggctttaat tcatcataaa atgcttggaa ttcatctaaa tccatttgtt gacccgcatc
      421 actaagtgca acagatggat ctggatgcac ctcagccata actccatcag caccaactgc
      481 taatgctgct ttcgcagttg gtaacatgat atctttacga cctgtactat gcgtaacatc
      541 taccatgact ggtaagtgtg taccttgttt taaaattggt actgctgaaa tatctaaagt
      601 gttacgtgtc gccttttcat aagttcggat tccacgttca cataaaataa tgttttgatt
      661 accttgtgaa gcaatgtatt cagctgcata aacaaactct tcgattgtag cagataaacc
      721 acgttttaat agaataggct ttttcgtacg gccagcttct tttaataact cgaagttttg
      781 catattacgt gcaccaattt ggaatacgtc taaatactca tcagccactt caaaatcatt
      841 tggatttacg atttcgctga caacatttaa atcatattta tctttaatct gtttaagtat
      901 tttaagtcct tcaacaccta ggccttggaa atcatatggt gatgtacgtg gtttaaatgc
      961 accgccacga ataaattttt cacctttagc atgtaagttt ttagcaacag cttcaacttg
     1021 ttcaaatgat tcaactgaac atggcccaaa tacaaatgat ttattgccgt ctccaataat
     1081 gcccccatta tcaaatgtta caatcgtatc ttcaggtttc aacttacgtg atacgtataa
     1141 gtgtttttca ttttcagatt tttgtaaatc tgtagaggct ttaaaaattt ctttaaataa
     1201 ttgcttaatt gtattatcgt taaatggtcc tttgttgcta tcaattaagt cgttaagcat
     1261 ttctttttcg cgttgtggat catagatacg tgtaccttgt tttaattttt cctccccaat
     1321 tttttgtgct agttcaccac gtttagataa taaatctaaa atttgatgat tcagtgagac
     1381 aatctcactt ctgtatgatt ctaatttatt actcatcctt actcacctct acgaattatt
     1441 tcaagtatat tccactttaa agcaataaaa tataatgtta aaaaatattc taacaatatc
     1501 atatgtatag ttcaacccta aattgacaga aaataatcaa ttttctaatt atatgattcg
     1561 agactgacat ttcgattttc tcagtcatta tt
//
LOCUS       DANBWH010000334         1590 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.345, whole genome shotgun sequence.
ACCESSION   DANBWH010000334 DANBWH010000000
VERSION     DANBWH010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.345"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            100..657
                     /locus_tag="PPY92_002744"
     CDS             100..657
                     /locus_tag="PPY92_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015978304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HDJ2626670.1"
                     /translation="MNKKLLTKTLIASALVLTTVGSGFHSSSNYNGINNVAKAEQTTD
                     RDLWKNVRDALKEANIIDNTANETVGVTYNLNNGAEHSITGTADLDELSTSNNSTVNT
                     DSVNSVDITRVNPNGKTIDANEAWKKLTDKLKEKNIVKDGDTVTIHSNDKGDPQISAK
                     VGSDYNGNNRFMLNQRDITKITITK"
     gene            complement(735..1535)
                     /gene="seb"
                     /locus_tag="PPY92_002745"
     CDS             complement(735..1535)
                     /gene="seb"
                     /locus_tag="PPY92_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type B"
                     /protein_id="HDJ2626671.1"
                     /translation="MYNRLFVSRVILIFALILVIYTPNVLAESQPDPKPDELHKASKF
                     TGLMENMKVLYDDNHVSAINVKSIDQFLYFDLIYSIKDTKLGNYDNVRVEFKNKDLAD
                     KYKDKYVDVFGANYYYQCYFSEKTNDINSHQTDKRKTCMYGGVTEHNGNHLDKYRSIT
                     VRVFEDGKNLLSFDVQTNKKKVTAQELDYLTRHYLVKNKKLYEFNNSPYETGYIKFIE
                     SENSFWYDMMPAPGDKFDQSKYLMMYNDNKLVDSKDVKIEVYLTTKKK"
BASE COUNT          576 a          242 c          220 g          552 t
ORIGIN      
        1 tttatagttt tgttaataat aagttaattg atttccccaa ataatgattg taaatttgat
       61 ttgtaatcga ttgcaaaata aatcatagga gaaaataaaa tgaataaaaa actattaaca
      121 aaaacattga tagcaagtgc tttagtttta acaacagtag gttcaggttt tcattcttct
      181 tcaaattata atggtattaa taacgttgca aaagctgagc aaacgacaga tagagactta
      241 tggaaaaatg taagagatgc tttgaaagaa gcaaatatta tcgataatac agcaaatgag
      301 acagttggtg ttacgtacaa tttaaataat ggtgctgagc atagcattac tggcactgcc
      361 gatttggacg agcttagtac ttctaataat agtactgtta acactgatag tgttaacagt
      421 gtcgatatta caagagttaa tccaaacgga aagacaatag atgctaatga agcttggaaa
      481 aaattaacag ataaattaaa agaaaagaat atcgttaaag acggcgatac agtaactatt
      541 catagtaatg ataaaggtga tccccaaatt tcagctaaag ttggaagtga ctataatggc
      601 aacaaccggt tcatgctaaa tcagagagat ataacaaaaa taactataac taaataatat
      661 tagaaaaggc aggtactcta taagcgcctg ccttaattac taactcttca tatttacttt
      721 tctaaaatat aatttcactt tttctttgtc gtaagataaa cttcaatctt cacatcttta
      781 gaatcaacca atttattatc attgtacatc attaaatatt tagattggtc aaatttatct
      841 cctggtgcag gcatcatgtc ataccaaaag ctattctcac tttctataaa tttaatatat
      901 cccgtttcat aaggtgagtt gttaaattca tagagttttt tatttttcac caaatagtga
      961 cgagttaggt aatctaattc ttgagcagtc acttttttct tattagtttg tacgtcaaaa
     1021 gacaataaat ttttaccatc ttcaaatacc ctaacagtaa tacttctata tttatctaaa
     1081 tggtttccat tatgctcagt tacaccacca tacatacaag tttttcgttt atcagtttga
     1141 tgtgaattaa tatcattcgt tttttcagaa aaataacatt gataataata attagctcca
     1201 aacacatcta cgtatttatc tttgtattta tcagctaaat ctttgttttt aaattcgact
     1261 cgaacattat cataattccc taacttagtg tccttaatag aatatattaa gtcaaagtat
     1321 agaaattgat ctatagattt aacgtttatt gctgatacat gattatcatc gtataaaact
     1381 ttcatatttt ccatcaaacc agtgaattta ctcgctttgt gcaactcatc tggtttagga
     1441 tctggttggc tttctgctaa aacgttgggt gtataaataa ctagtatcag tgcgaatatc
     1501 aaaattacac gtgaaacaaa taatctatta tacatttttt atctccttta tccaacattc
     1561 ccaaaaagta tctagataca ctttaaatat
//
LOCUS       DANBWH010000335         1584 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.346, whole genome shotgun sequence.
ACCESSION   DANBWH010000335 DANBWH010000000
VERSION     DANBWH010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.346"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            320..1192
                     /locus_tag="PPY92_002746"
     CDS             320..1192
                     /locus_tag="PPY92_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDJ2626672.1"
                     /translation="MSNVLTEIDSQYPYMTKNEKKIAKFILNSPQKVIKMRSQDLASL
                     LDISTSSVIRFSKKITDGGFHDLKINISKYVPKASSIYNVELMNNESTESLRTKLHTR
                     TTRALNHANNELNDKTIDQICHCLKRSETIFIYGFGASFVVATDLYQKLSRIGLNIQL
                     VQETHIFATLLATHNSNDSVILITNNGTQSEMQSMVKVIDDYHIPIITITSTRDNPVA
                     QASNIVLTYGKTDENEMHMGATTSLFAQMFTIDILYYRYVALNYHASLDFITQSKMAL
                     DNYRKHLSNINFKH"
BASE COUNT          597 a          243 c          197 g          547 t
ORIGIN      
        1 ttaagatggt agatttagtc ttatataact acatttacta agatttaaat agatgagtac
       61 ataatgtata aattgggaat atacagtatg acttacggta atatatcaat atattgatat
      121 attatttgca catgggagta tgctattttt tatctataca tcttaaaaaa atttacagtt
      181 ttacaattca atatcattaa acaaacaaca tattcagctt atttttcatt ctcttacgtt
      241 acaagttgta aataccatcg cgttaaacaa caatagccaa cgctaataaa atatattgcg
      301 ttaaagtgga gtgattaata tgtcaaacgt actaacagaa atagatagtc aatatccata
      361 tatgactaaa aatgaaaaaa agatagctaa atttatacta aattcacctc aaaaggtgat
      421 taaaatgagg tctcaagact tagcaagctt attagatatt agtacatcat cagtcattcg
      481 attcagcaag aaaattactg atggcggttt tcacgacttg aaaataaata tatctaaata
      541 tgttcctaaa gcatcatcaa tttacaatgt tgaattaatg aataacgaaa gcacagaatc
      601 tttaagaact aaactccata cacgtactac acgcgcactt aatcatgcaa ataacgaatt
      661 aaatgataaa actattgatc aaatatgtca ttgtttaaaa cgttctgaga cgatttttat
      721 atatggtttt ggcgcatctt ttgtagttgc taccgactta taccaaaagt tatcaagaat
      781 aggtcttaat attcaacttg ttcaagaaac acatattttt gctacattat tagcaactca
      841 caattcgaac gacagtgtta ttcttattac taacaatggt acgcaaagtg aaatgcaatc
      901 aatggttaaa gttattgatg actaccatat acctataatt acaattacta gtacaaggga
      961 taatcccgta gcacaggcat caaatattgt tttaacttat gggaaaactg atgaaaatga
     1021 gatgcatatg ggagcaacaa cttcattatt tgctcaaatg ttcactattg atattttata
     1081 ttatcgttat gttgcattaa attatcacgc atcattagat ttcataacac aatctaaaat
     1141 ggctttagat aactatcgta aacatttatc aaatattaac tttaaacatt aacagtctta
     1201 tataaattct ttaatcatgt catataataa tttatgcatc attttcaaag cttacatgtc
     1261 ttttatcttg tataacatac gcatatgacc aatgatttga taaggccatt caatttctta
     1321 tattaaaaac aaacgcagtt gattacagga ttcattccat atatcaattg cgtttgttat
     1381 tttaataatg ctatttttta ttttaaagcc cacagtaaaa actaacgata atattgccac
     1441 ttataagttt ttcacactac aacttcgatt taagctatat ttataaaaaa atgcgcctac
     1501 tcataacatt ctttggctat ttaactttaa taagctaggg ccaaatttgt cataaaataa
     1561 gcgcacatta taatcatact attt
//
LOCUS       DANBWH010000336         1581 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.347, whole genome shotgun sequence.
ACCESSION   DANBWH010000336 DANBWH010000000
VERSION     DANBWH010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.347"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            234..356
                     /locus_tag="PPY92_002747"
     CDS             234..356
                     /locus_tag="PPY92_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626673.1"
                     /translation="MSYIIKFTFKFFEMATYISQLTLDIELVSSELLVILPGFI"
     gene            complement(360..1154)
                     /locus_tag="PPY92_002748"
     CDS             complement(360..1154)
                     /locus_tag="PPY92_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDJ2626674.1"
                     /translation="MIHSKKLTLGICLVLLIILIVGYVIMTKTNGRNAQIKDTFNQTL
                     KLYPTKNLDDFYDKEGFRDQEFKKGDKGTWIVNSEMVIEPKGKDMETRGMVLYINRNT
                     RTTKGYYFISEMTDDSNGRPKDDEKRYPVKMEHNKIIPTKPLPNDKLRKEIENFKFFV
                     QYGDFKDINDYKDGDISYNPNVPSYSAKYQLKNDDYNVKQLRKRYNIPTNKAPKLLIK
                     GDGDLKGSSVGSKNLEFTFVENKEENIYFTDSVQYTSSEDTSYESN"
     gene            complement(1266..1373)
                     /locus_tag="PPY92_002749"
                     /pseudo
     CDS             complement(1266..1373)
                     /locus_tag="PPY92_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649608.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
BASE COUNT          473 a          266 c          201 g          641 t
ORIGIN      
        1 aatgtagatg ttctttagac attaaaagct tctaacatga tacggaatat gaggttcctg
       61 tatcacatta gaagcttttt tgttgcggtg cttatattaa taatggcatg aatgaacgca
      121 tgctgtctta gtttcaggaa tcggctagaa tattaaactt ccgttctttg aatatttttt
      181 aatttcttac taatatcatc cagttcattc ttcgcctttt taacccaatc tccttgagct
      241 acattatcaa attcaccttt aaattttttg aaatggctac ctatatttca cagctcactt
      301 tagatatcga acttgtttct tcagagttac ttgttatatt accaggtttt atttgatatt
      361 cagtttgact cataacttgt atcctcactt gaagtatatt gtacagaatc tgtaaaataa
      421 atgttttctt ctttattttc tacaaaggta aattctagat tttttgaccc tacagatgag
      481 ccttttaaat caccatcacc ttttattaat aatttcggag ctttgttggt tggaatatta
      541 tatctttttc gaagttgctt cacattgtag tcatcgtttt tcaattgata ttttgcggaa
      601 taacttggaa cattaggatt atatgaaatg tcaccatctt tataatcatt aatatcttta
      661 aagtcaccgt attgtacaaa gaatttaaag ttttcgattt cttttcttaa cttgtcattc
      721 ggtagtggct tcgttggtat gattttatta tgttccattt ttaccggata ccttttttca
      781 tcatcctttg gtctgccgtt actgtcatct gtcatttcac ttataaaata ataccctttt
      841 gtggttctag tattgcgatt gatatagagc accattcctc tcgtttccat atccttacct
      901 tttggctcga ttaccatttc agaattaact atccaagtac ctttatcacc ctttttaaat
      961 tcttgatctc gaaagccttc tttatcgtaa aaatcatcga gatttttggt tggatataat
     1021 tttaatgttt gattaaatgt gtctttaatt tgggcgtttc gaccatttgt ttttgtcata
     1081 atgacataac ctacaatcaa tataatgagt aataccaagc atataccgag cgttaatttc
     1141 tttgaatgaa tcatcatttc tccaccttat atttttaata ttgactcaat ctttaattta
     1201 tacaactaaa gcgaaaaatt attttgaaca ctttatgaaa tttttatttc aaacttctat
     1261 taaccctatt cagtaggttt gaaattaata ctatcagaaa aatacacatt ttcttcttta
     1321 tttcttataa agttaaattc tagttcttta gaacctacgg atgagccttt taatcgccat
     1381 caccttcata cagtttgaag tcatatttac caattgtaaa tgacgactag atgatatata
     1441 gttcaagaaa catcaaaatt cgttaaaaca taatcaaatt ttattatgtt caaaacttta
     1501 ttttccaaaa aataaaaaag aagttaaaca aagattggtt gcttaacttc cattattgaa
     1561 ttcattagtc cgatttgaca t
//
LOCUS       DANBWH010000337         1516 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.348, whole genome shotgun sequence.
ACCESSION   DANBWH010000337 DANBWH010000000
VERSION     DANBWH010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.348"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            51..839
                     /locus_tag="PPY92_002750"
     CDS             51..839
                     /locus_tag="PPY92_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDJ2626675.1"
                     /translation="MNKVTINPQIQLTYQIEGKGDPIILLHGLDGNLAGFEDLQHQLA
                     SSYKVLTYDLRGHGKSSKSESYDLNDHVEDLKILMEKLNIHEAHILGHDLGGVVAKLF
                     TDKYAYRVKSLTTIASKKDDLIHSFTQLLIQYQDDIAGFNKSEAYILLFSKLFRNQEK
                     TMKWYQKQRIYSIKSEDDSAVAIRSLILHKDEPMYLKKRTCVPTLLINGEHDPLIKDK
                     NHFKLEAHFLNVTKKIFEHSGHAPHIEEPEAFMNYYLNFLKSVS"
     gene            complement(1091..1465)
                     /locus_tag="PPY92_002751"
     CDS             complement(1091..1465)
                     /locus_tag="PPY92_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HDJ2626676.1"
                     /translation="MAITKINDCFELLSMVTYADKLKSLIKKEFSISFEEFAVLTYIS
                     ENKEKEYYLKDIINHLNYKQPQVVKAVKILSQEDYFDKKRNEHDERTVLILVNAQQRK
                     KIESLLSRVNKRITEANNEIEL"
BASE COUNT          528 a          207 c          245 g          536 t
ORIGIN      
        1 cgtatataga atgaaggttg ggtatatagt ttattgaggg aggtgtcaca atgaataaag
       61 tcacaattaa tcctcaaatc caattaactt atcaaattga aggtaaaggg gatcctataa
      121 tattacttca tggattggat ggtaatttag ctggatttga agatttgcaa catcaactag
      181 catcatcata taaagtactt acttacgatt taagaggtca tggcaagtct tctaaaagtg
      241 aatcatacga tttaaacgat cacgttgagg atttaaaaat tctaatggag aagttaaata
      301 ttcatgaggc acatattcta ggacatgatt taggtggggt agttgctaag ttatttacag
      361 ataaatatgc ttatcgtgta aaatcattaa ctaccattgc atcgaagaaa gatgacttaa
      421 tacacagctt tactcaattg ttaatacaat atcaagatga tatagcgggt tttaataagt
      481 ctgaagcgta tattctttta ttttctaaat tgtttagaaa tcaagagaag acgatgaaat
      541 ggtatcaaaa acaaagaata tatagcatta agtctgagga tgatagtgcg gtggcaattc
      601 gttcattaat tttgcataaa gatgaaccta tgtatttaaa aaaacgtaca tgtgtaccta
      661 ctttgttaat taatggggaa catgatcctt tgattaaaga taagaatcat tttaaattgg
      721 aagcgcattt tttaaatgtt acgaaaaaaa tcttcgaaca ttcaggacat gcaccgcata
      781 ttgaagaacc agaagcattt atgaattatt atttaaattt tttaaaaagc gtatcataat
      841 atgtgatata taaacctagg gcataaagtc cttaggcaat gtgaaaaagc tgattactat
      901 tcattatttg atagaaatca gctttttttg aaatgtattt gatatatact gctcgttatg
      961 cggctatctt ccttatatta agtgccatta gtgcaaaacc tcttaacaat taggtaaaaa
     1021 aagacataaa aaaaggaagt ttaatagaat gtatcatcta tcaaacttca ccaaattgcg
     1081 ctaaacaaaa ttatagttca atttcgttgt ttgcttcagt gattcgttta tttactcgac
     1141 tcaataatga ttcgattttt ttacgttgtt gtgcattaac aagaattaat acagttcttt
     1201 catcatgctc attacgtttt ttatcgaagt aatcttcttg agataaaatt ttaactgctt
     1261 taacaacttg tggttgtttg tagtttaaat gattaataat atctttaaga tagtattctt
     1321 tctctttgtt ttcgctgatg tatgtcaata cagcgaattc ttcaaagcta attgaaaatt
     1381 cctttttaat taaacttttt aatttgtcag cataagtgac cattgataac aactcaaagc
     1441 aatcattgat ttttgtaatt gccatgttta aaacctccct atttgatgca tcttgctcga
     1501 tacatttgcc cgataa
//
LOCUS       DANBWH010000338         1512 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.349, whole genome shotgun sequence.
ACCESSION   DANBWH010000338 DANBWH010000000
VERSION     DANBWH010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.349"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..1492
                     /locus_tag="PPY92_002752"
     CDS             <1..1492
                     /locus_tag="PPY92_002752"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019142.1,HMM:NF028351.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="E domain-containing protein"
                     /protein_id="HDJ2626677.1"
                     /translation="ETKRVLDPTMEPGSPDKVAQKGENGEKTTTTPTTINPLTGEKVG
                     EGEPTTEVTKEPIDEIVNYAPEIIPHGTREEIDPNLPEGETKVIPGKDGLKDPETGEI
                     IEEPQDEVIIHGAKDDSDSDADSDSDADSDSDADSDSDSDSDSDADSDSDADSDSDAD
                     SDSDADSDSDADSDSDADSDSDADSDSDADSDSDADSDSDADSDSDADSDSDADSDSD
                     ADSDSDADSDSDSDSDSDADSDSDADSDSDADSDSDADSDSDSDSDSDADSDSDADSD
                     SDSDSDSDADSDSDADSDSDADSDSDADSDSDADSDSDADSDSDADSDSDSDSDSDAD
                     SDSDSDSDSDADSDSDADSDSDADSDSDADSDSDADSDSDSDSDSDADSDSDADSDSD
                     ADSDSDADSDSDADSDSDADSDSDSDSDSDADSDSDADSDSDADSDSDADRDHNDKTD
                     KPNNKELPDTGNDAQNNGTLFGSLFAALGGLFLVGRRRKNKNNEEK"
BASE COUNT          520 a          326 c          381 g          285 t
ORIGIN      
        1 tgaaactaaa cgcgtattag atccaacaat ggaaccaggt agtcctgata aagtagctca
       61 aaaaggtgaa aatggtgaaa aaacaacaac aacaccaact acaattaatc cattaacggg
      121 agaaaaagta ggcgaaggcg aaccaacaac ggaagtaacg aaagaaccaa tagacgaaat
      181 tgttaactat gcacctgaaa ttattcctca tggtacacgt gaagaaattg atccaaactt
      241 accagaaggt gaaactaaag ttatcccagg taaagatggc ttgaaagatc ctgaaactgg
      301 agaaatcatt gaagaaccac aagatgaagt aatcatccat ggtgctaaag atgattcaga
      361 ttcagatgcg gacagcgact cagacgcaga tagcgattca gatgcggaca gcgactcaga
      421 ttcagacagc gactcagacg cagatagcga ctcagacgca gatagcgact ctgatgcgga
      481 cagcgactca gacgcagata gcgattctga tgcggacagc gattcagacg cagatagcga
      541 ttctgatgca gacagcgact cagacgcaga tagcgattca gatgcggaca gcgattcaga
      601 cgcagatagc gattcagatg cggacagcga ttcagacgca gatagcgatt ctgatgcgga
      661 cagcgattca gacgcagata gcgattcaga ttcagatagc gactctgatg cggacagcga
      721 ctcagacgca gatagcgact cagacgcaga tagcgactct gatgcggaca gcgactcaga
      781 ttcagacagc gattcagacg cagatagcga ctctgatgcg gacagcgact cagattcaga
      841 cagcgattca gacgcagata gcgattctga tgcagacagc gactcagacg cagatagcga
      901 ctctgatgcg gacagcgact cagacgcaga tagcgactca gacgcagata gcgactctga
      961 tgcggacagc gactcagatt cagacagcga ttcagacgca gatagcgatt cagattcaga
     1021 cagcgactca gacgcagata gcgattcaga tgcggacagc gattcagacg cagatagcga
     1081 ttctgatgcg gacagcgatt cagacgcaga tagcgattca gattcagata gcgattctga
     1141 tgcggacagc gattcagacg cagatagcga ttcagatgcg gacagcgatt cagacgcaga
     1201 tagcgattca gatgcggaca gcgattcaga cgcagatagc gattcagatt cagatagcga
     1261 ctctgatgcg gacagcgact cagacgcaga tagcgactct gatgcggaca gcgactcaga
     1321 cgcagataga gatcataatg acaaaacaga taaaccaaat aataaagagt taccagatac
     1381 tggtaatgat gctcaaaata atggcacatt atttggttca ctattcgctg cgcttggagg
     1441 attattctta gttggcagac gtcgtaaaaa caaaaataat gaagaaaaat aatatttaaa
     1501 cttcataatt tt
//
LOCUS       DANBWH010000339         1475 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.350, whole genome shotgun sequence.
ACCESSION   DANBWH010000339 DANBWH010000000
VERSION     DANBWH010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.350"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..793)
                     /locus_tag="PPY92_002753"
     CDS             complement(<1..793)
                     /locus_tag="PPY92_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDJ2626678.1"
                     /translation="MQPHLICLDLDGTLLNDNKEISSYTKQVLNELQQRGHQIMIATG
                     RPYRASQMYYHELNLTTPIVNFNGAYVHHPKDKNFKTCHEILDLGIAQNIIQGLQQYQ
                     VSNIIAEVKDYVFINNHDPRLFEGFSMGNPRIQTGNLLVHLKESPTSILIEAEESKIP
                     EIKNMLTHFYADHIEHRRWGAPFPVIEIVKLGINKARGIEQVRQFLNIDRNNIIAFGD
                     EDNDIEMIEYARHGVAMENGLQELKDVANNITFNNNEDGIGRYLND"
     gene            907..1215
                     /locus_tag="PPY92_002754"
     CDS             907..1215
                     /locus_tag="PPY92_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HDJ2626679.1"
                     /translation="MEEALKDSILGALEMVIDPELGIDIVNLGLVYKVNVDDEGVCTV
                     DMTLTSMGCPMGPQIIDQVKTVLAEIPEIQDTEVNIVWSPPWTKDMMSRYAKIALGVS
                     "
BASE COUNT          461 a          222 c          262 g          530 t
ORIGIN      
        1 aatcattcaa atatcgacca atgccatctt cattattgtt gaatgtaata ttgttcgcta
       61 catctttaag ttcttgcaaa ccattttcca tagcaacacc atggcgggcg tactcaatca
      121 tttcaatatc attatcttca tcaccgaatg caataatatt atttcggtca atatttaaaa
      181 attgtctaac ttgctcaatg cctcttgctt tattaatacc aagttttaca atttcaatga
      241 cagggaatgg tgcgccccag cgtcgatgct caatatgatc ggcataaaaa tgagtaagca
      301 tatttttgat ttcaggtatt ttactttctt cggcttcaat taaaattgag gtaggggatt
      361 ctttcaagtg gacaagtaaa ttaccagttt gaattcttgg attacccatt gaaaaacctt
      421 caaataatct tggatcatga ttgttaatga aaacataatc tttcacttct gctataatat
      481 tcgatacttg atattgttgt aatccttgaa taatgttttg tgcgatgcct aaatctaaaa
      541 tttcatggca agttttgaag tttttatctt tagggtgatg tacgtaagcg ccattaaaat
      601 taacaattgg tgtcgttaaa tttaattcat gataatacat ttgacttgca cgataaggtc
      661 tgccagtcgc aatcataatt tggtgtccac gttgttgtaa ttcatttaat acttgtttag
      721 tatatgatga aatttctttg ttatcgttta ataatgttcc gtctaagtct agacatatta
      781 aatgtggttg catatctctc tcctcataag ttgcttagtt catatagata catcatagca
      841 ttttattggc gttttgtcgg atatttgata tattatgaat aatatgacat taaagaggtg
      901 atatggatgg aagaggcatt gaaagatagt atcttaggtg cattagaaat ggtaattgac
      961 cctgaattag gaattgatat cgttaattta ggtttagtat acaaagtgaa tgttgatgat
     1021 gaaggcgtat gtacagttga tatgacttta acatcaatgg gatgtccaat gggacctcaa
     1081 attattgatc aagttaaaac agtattagca gagattcctg aaattcagga tactgaagtg
     1141 aatatcgtat ggagtccacc ttggacaaaa gatatgatgt cacgttacgc taagattgca
     1201 cttggtgtga gctaacataa acgtgcattg cctgtagaat ttcttttaga aattctctgt
     1261 gttgggtccc cgcagtcaat ataacattgt agagtataat tcattgtgaa taagctccct
     1321 tcaaagtaga cattgaaaaa tgaaaatttt gaagggagcc ttatttatgt tctaggttct
     1381 gatttcattt tgaaaattta tatctaactt gtttcaaaaa atatgaatta agacagtgac
     1441 ttgcacttcc aagagtgcac aattcatttt ctagt
//
LOCUS       DANBWH010000340         1471 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.351, whole genome shotgun sequence.
ACCESSION   DANBWH010000340 DANBWH010000000
VERSION     DANBWH010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.351"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            102..785
                     /locus_tag="PPY92_002755"
     CDS             102..785
                     /locus_tag="PPY92_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL11"
                     /protein_id="HDJ2626680.1"
                     /translation="MKLKNIAKASLALGILTTGMITTTAQPVKASTLEVRSQATQDLS
                     EYYKGRGFELTNVTGYKYGNKVTFIDNSQQIDVTLTGNEKLTVKDDDEVSNVDVFVVR
                     EGSDKSAITTSIGGITKTNGTQHKDTVQNVNLSVSKSTGQHTTSVTSEYYSIYKEEIS
                     LKELDFKLRKHLIDKHDLYKTEPKDSKIRITMKNGGYYTFELNKKLQPHRMGDTIDSR
                     NIEKIEVNL"
     gene            807..>1471
                     /locus_tag="PPY92_002756"
     CDS             807..>1471
                     /locus_tag="PPY92_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKLRK protein"
                     /protein_id="HDJ2626681.1"
                     /translation="MRENFKLRKMKVGLVSVAITMLYIMTNGQAEASETNQKVSTNQE
                     SKAVSQTEQNSKETKAAESSKNFVNLDPIKPGAQKVTGTTLPNHIILLNIDGKSADSV
                     EGGYGDFITANDKGEFEYPLNNRKIVHNQEIEVSSSSPDLGDDEEDEEVEESSTEKAG
                     AEEESTDAKATYTTPRYEKAYEIPKEQLKEKDGHHQVFIEPITEGSGIIKGHTSVKGK
                     VALS"
BASE COUNT          604 a          196 c          272 g          399 t
ORIGIN      
        1 tttaatgtga agcacgacca ttgctcatta taatgaatga ggattgttcg tattgcgtaa
       61 tagaataaat caaatagact aaaaattggg agcatagaat tatgaaatta aaaaatattg
      121 ctaaagcaag tttagcacta gggattttaa caacaggtat gattacaact actgctcagc
      181 cagtaaaagc aagtacatta gaggttagat cacaagctac tcaagacttg agtgaatatt
      241 ataaagggag aggatttgaa ttaacaaatg taacaggata taaatacgga aataaagtta
      301 catttataga taattctcaa caaattgatg ttacattgac aggaaatgaa aaattaactg
      361 ttaaagatga tgacgaagtt tctaatgttg acgtgtttgt agtaagagaa ggtagtgaca
      421 aatcagctat cacaacatcg attggtggaa ttacaaagac aaatgggact caacataaag
      481 atactgttca aaacgttaat ttgtcagttt ctaagagtac aggtcaacac actacttctg
      541 tgacttcaga atactatagc atctataaag aagaaatctc attaaaagaa cttgatttta
      601 aattaagaaa gcatttaatt gataaacatg atctttataa gacagaacct aaagacagta
      661 aaattagaat tactatgaaa aacggtggct attatacatt tgaattaaat aaaaaattac
      721 agcctcatcg catgggtgat acgattgata gtagaaatat agagaaaatt gaagtgaatt
      781 tataataata ttcgagggag tatatcatga gagaaaattt taaattacgt aaaatgaaag
      841 tcggtttagt atcagttgca attactatgt tatatatcat gacaaacgga caagctgaag
      901 catctgaaac taatcagaag gtaagtacaa atcaagaatc aaaagctgtt tcgcagactg
      961 aacaaaattc caaagaaaca aaagcggcag agtcttctaa aaactttgta aatttagatc
     1021 ctatcaaacc aggagctcaa aaagtaacag gtactacatt accgaatcat attattttac
     1081 ttaatataga tggtaaaagt gctgattcag ttgaaggagg atatggtgat tttataactg
     1141 caaatgacaa aggagagttt gagtatcctt taaataatcg taaaattgtc cataaccaag
     1201 aaattgaggt atcatcatca agccctgatt taggtgacga tgaagaggat gaagaagtag
     1261 aagaatcttc aactgaaaaa gctggtgctg aggaagaaag tacagatgct aaagctacat
     1321 atacaacacc acgatatgaa aaagcgtatg aaataccgaa agaacaacta aaagaaaaag
     1381 atggacacca ccaagttttc atcgaaccaa ttactgaagg ttcaggtatt attaaaggtc
     1441 atacgtctgt aaaaggtaaa gttgctctat c
//
LOCUS       DANBWH010000341         1452 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.352, whole genome shotgun sequence.
ACCESSION   DANBWH010000341 DANBWH010000000
VERSION     DANBWH010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.352"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            58..>1452
                     /gene="eap"
                     /locus_tag="PPY92_002757"
                     /gene_synonym="map"
     CDS             58..>1452
                     /gene="eap"
                     /locus_tag="PPY92_002757"
                     /gene_synonym="map"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular adherence protein Eap/Map"
                     /protein_id="HDJ2626682.1"
                     /translation="MKFKSLITTTLALGVIASTGANFNTNEASAAAKPLDKSSSTLHH
                     GHSNIQIPYTITVNGTSQNILSSLTFNKNQNISYKDIENKVKSVLYFNRGISDIDLRL
                     SKQAEYTVHFKNGTKRVIDLKSGIYTADLINTSDIKAISVNVDTKKQPKDKAKANVQV
                     PYTITVNGTSQNILSNLTFNKNQNISYKDLEGKVKSVLESNRGITDVDLRLSKQAKYT
                     VNFKNGTKKVIDLKSGIYTANLINSSDIKSININVDTKKHIENKAKRNYQVPYSINLN
                     GTSTNILSNLSFSNKPWTNYKNLTSQIKSVLKHDRGISEQDLKYAKKAYYTVYFKNGG
                     KRILQLNSKNYTANLVHAKDVKRIEITVKTGTKAKADRYVPYTIAVNGTSTPILSKLK
                     ISNKQLISYKYLNDKVKSVLKSERGISDLDLKFAKQAKYTVYFKNGKKQVVNLKSDIF
                     TPNLFSAKDIKKIDI"
BASE COUNT          636 a          198 c          206 g          412 t
ORIGIN      
        1 aagtctggct ataataaagt tgcaatcacg aattacataa aaaaggagag ataatttatg
       61 aaatttaagt cattgattac aacaacatta gcattaggcg ttatagcatc aacaggagca
      121 aactttaata ctaacgaagc atctgccgca gctaagccat tagataaatc atcaagtaca
      181 ttacaccatg gacattctaa catccagatt ccatatacaa ttactgtgaa cggtacaagc
      241 caaaacattt tatcaagctt aacatttaat aagaatcaaa atattagtta taaagatata
      301 gagaataaag ttaaatcagt tttatacttt aatagaggta ttagtgatat cgatttaaga
      361 ctttcaaagc aagcggaata tacggttcat tttaaaaatg gaacaaaaag agttatcgat
      421 ttgaaatcag gtatctacac agctgactta atcaatacaa gtgacattaa agctatcagt
      481 gttaacgtag atactaaaaa gcaacctaaa gataaagcta aagcaaatgt tcaagtgcca
      541 tatacaatca cagtgaacgg cacaagccaa aacattttat caaacctaac atttaataaa
      601 aatcaaaata ttagttacaa agatttagag ggtaaagtta aatcagtttt agaatcaaat
      661 agaggtatta ctgatgttga tttaagactt tcgaagcaag cgaaatatac agttaatttt
      721 aaaaatggaa cgaagaaagt tatcgatttg aaatcaggta tttacacagc gaatttaatc
      781 aattcaagtg atattaaaag tatcaatatt aacgtagata caaaaaaaca tatcgaaaat
      841 aaagctaaaa gaaactatca agttccatat tcaattaatc taaatggtac atctacaaac
      901 attttatcga atctttcatt ttcaaataaa ccttggacaa attacaaaaa tttaactagt
      961 caaataaaat cagtactgaa gcatgataga ggtattagtg aacaagattt aaaatatgct
     1021 aagaaagctt attatactgt ttattttaaa aatggtggta aaagaatctt acagttaaat
     1081 tcaaaaaatt acacagcaaa cttagttcat gcgaaagatg ttaagagaat tgaaattact
     1141 gttaaaacag gaactaaagc gaaagcagac agatatgtac catacacaat tgcagtaaat
     1201 ggcacatcaa caccaatttt atcaaaacta aaaatttcga ataaacaatt aattagttac
     1261 aaatatttaa acgacaaagt gaaatctgta ttaaaaagtg aaagaggtat cagtgatctt
     1321 gacttaaaat ttgcgaaaca agcaaaatat acagtatatt tcaaaaatgg aaagaaacaa
     1381 gtagtgaatt taaaatcaga catctttaca cctaatttat ttagtgccaa agatattaaa
     1441 aagattgata tt
//
LOCUS       DANBWH010000342         1437 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.353, whole genome shotgun sequence.
ACCESSION   DANBWH010000342 DANBWH010000000
VERSION     DANBWH010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.353"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..411
                     /gene="sigS"
                     /locus_tag="PPY92_002758"
     CDS             <1..411
                     /gene="sigS"
                     /locus_tag="PPY92_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigS"
                     /protein_id="HDJ2626683.1"
                     /translation="YNISYNYDEYYQLLLIKMWQLSQIYKPSSKQSLSSFLFTRLNYY
                     LIDLFRQQNQLKDVILCENNSPTLTEQPTYFNEHDLRLQDIFKLLNHRERLWLKLYLE
                     GYKQFEIAEIMSLSLSTIKLIKMSVKRKCQHNFN"
     gene            524..967
                     /locus_tag="PPY92_002759"
     CDS             524..967
                     /locus_tag="PPY92_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HDJ2626684.1"
                     /translation="MQDNSTKYLLYIQTATSNHLETNCVFLHCDYILKVPINKLVSYY
                     AKLHLSSQSVLIETAKNILNINKLVPIYINPKTILFPLKHKRAPIQIYINAHYIVGMT
                     AIENSTLIHFQEGIQLEVDEPFSLVSKKCHESLALKHFIENTISN"
     gene            complement(954..1397)
                     /locus_tag="PPY92_002760"
     CDS             complement(954..1397)
                     /locus_tag="PPY92_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626685.1"
                     /translation="MNKASFDKKVKKQLWFLNKKEKQALDQRLSSISDDDSVNLNKPV
                     TFANAYLRQNVFRNKETKSYSMFVTLVVMMFAYVALLGLFLFGLITSLSGVQFFVSPK
                     VDLSTTVVILTIIGAILLMIVSIYFIKIVTSYFTKKLLEIKFNSK"
BASE COUNT          531 a          237 c          167 g          502 t
ORIGIN      
        1 tataatatta gctataatta tgatgagtat tatcaactac tcttgataaa aatgtggcaa
       61 ttgagtcaga tatataaacc ctcaagcaag caatctttat cctctttttt attcactcga
      121 ttaaattatt accttatcga tttattcaga caacaaaatc aattaaaaga tgtcatttta
      181 tgtgagaata attcaccaac attaactgaa caaccaacat actttaatga acatgacctt
      241 cgtttacaag atatcttcaa gcttttaaat catagagaaa gactatggct caaactatac
      301 cttgaaggat acaagcaatt tgaaattgct gaaatcatgt cattatcgct ttcaacgatt
      361 aaattaatta agatgtccgt taagcgtaaa tgccaacata attttaatta gtttattgtg
      421 aaatttcggt cgatgtgtta gtgatgttga aacaccctaa catcttcata taaatttatt
      481 ttaaccatta aattaacaac ttattatacg aggagtacta tatttgcaag acaattctac
      541 taaatatcta ctttatatcc aaactgctac ttcaaaccat ctcgaaacaa attgtgtctt
      601 tttacattgc gattacattc ttaaagttcc aattaacaaa ctcgtttcat attatgcgaa
      661 attgcattta tcatcacaaa gtgtgctaat tgagactgca aaaaacatac taaatattaa
      721 taaactggtt cctatttaca tcaacccaaa aactatactt tttccgttaa aacataaacg
      781 tgcaccgata caaatttata tcaatgcaca ttatattgtt ggtatgactg ccatagagaa
      841 ttcaacatta atacattttc aagaaggcat tcagctagaa gttgatgagc cattttctct
      901 agtttcaaaa aaatgtcatg aaagtttagc tttgaagcac ttcattgaaa atactatttc
      961 gaattaaatt taatttctaa aagtttctta gtaaagtatg atgtcacaat ttttataaaa
     1021 tatatgctaa caatcattaa taaaatcgct ccaataattg ttaaaataac aacagttgtt
     1081 gataaatcta ccttaggact aacgaaaaat tgtacgccag atagacttgt aattagccca
     1141 aataaaaaca agcctaataa agcaacataa gcaaacatca tcactactaa tgtaacaaac
     1201 atactataac tttttgtttc tttatttcga aatacatttt gtcttaagta agcattagca
     1261 aaggtaacag gcttatttaa gttaacgctg tcatcatcag atatagaaga taatcgttga
     1321 tctaatgctt gtttctcctt cttgttcaaa aaccataatt gctttttaac ttttttatca
     1381 aatgatgctt tattcataat taaggatttc tctcctttat aatattgacg taatttt
//
LOCUS       DANBWH010000343         1419 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.354, whole genome shotgun sequence.
ACCESSION   DANBWH010000343 DANBWH010000000
VERSION     DANBWH010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.354"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..346
                     /gene="fmhC"
                     /locus_tag="PPY92_002761"
     CDS             <1..346
                     /gene="fmhC"
                     /locus_tag="PPY92_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhC"
                     /protein_id="HDJ2626686.1"
                     /translation="ELILEDGPVLDLAAALFICTDDEVYYLSSGSNPKYNQYMGAYHL
                     QWHMIKYAKSHNINRYNFYGITGVFSNEADDFGVQQFKKGFNAHVEELIGDFIKPVRP
                     ILYKFAKLIYKV"
     gene            519..1391
                     /gene="dprA"
                     /locus_tag="PPY92_002762"
     CDS             519..1391
                     /gene="dprA"
                     /locus_tag="PPY92_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDJ2626687.1"
                     /translation="MIKLFLLKLYWAHFSTKQIHQFLMAYPNVIKEEGRKKDSYLCEW
                     VNREENVLLLRKYYAFIKLDHNDIIKELQKLKVSYITYMDSEYPVLLKEIYQFPLLLF
                     YKGNIKLINNMHHLAVVGARDSTSYTQQSLEFLLSNDKSKYLTIVSGLAQGADAMAHQ
                     IALKYNLPTIAVLAFGHQTHYPKSTLALRNKIEEKGLVISEYPPHTPIAKYRFPERNR
                     IISGLSKGVLITEAKEQSGSHITIDFALEQNRNVYVLPGSMFNPMTKGNLLRIQEGAK
                     VVLNANDIFEDYYI"
BASE COUNT          533 a          184 c          222 g          480 t
ORIGIN      
        1 agaactaata ttagaagatg gacctgtttt ggatttagct gctgctttat ttatatgtac
       61 tgatgatgaa gtttattatc tatcaagtgg atcaaatccg aaatataatc agtatatggg
      121 tgcatatcat ctacaatggc atatgataaa atatgcaaaa tcacataata ttaataggta
      181 taatttttat ggaataacag gcgtctttag taatgaggcg gatgattttg gtgttcaaca
      241 atttaaaaag ggttttaatg cacatgttga agaattaatt ggtgatttca tcaaaccagt
      301 aagaccaatt ctatataaat ttgcaaaact tatttataag gtttaattat aaagtatgtt
      361 ggaaattgaa attttaaatt ctttccaaca tacttttcac tttttaagtc agtattttta
      421 ctccttaaaa taacattaca tcgacaatgt ttttgtatta gaaatgtaaa acaaaaatag
      481 aatatgattt tgtatgatta caaaaaggag ttgatttatt gattaaacta tttttgctta
      541 agttatactg ggcacacttt tcgactaaac aaattcatca atttttaatg gcatatccta
      601 atgtaattaa agaggaggga agaaaaaaag atagttattt atgtgaatgg gtgaataggg
      661 aagaaaatgt tcttttatta cgtaaatact atgcttttat aaaacttgat cataacgata
      721 ttattaaaga actgcagaaa ttaaaagtaa gttacattac atatatggat tctgaatacc
      781 cagtgctatt aaaagaaata tatcaatttc cattacttct tttctataaa gggaacatca
      841 aattaataaa taatatgcat catttggcag tagtaggtgc aagagattct acaagttata
      901 cccaacagtc tttagaattt ttattatcaa atgataaaag caaatattta acaattgttt
      961 ccggccttgc tcaaggagct gatgcaatgg cacatcaaat agctttaaaa tacaatctcc
     1021 ctacaattgc agttttagcc tttggccatc aaacacatta tcccaaaagt acattagcat
     1081 taagaaataa aatagaagaa aaaggtttag ttatatccga atatccacca catacaccaa
     1141 ttgctaaata tagatttcct gagcgcaata gaattatcag cggtttgtca aaaggggttt
     1201 taattactga ggctaaggaa caaagtggca gtcacatcac gatagatttt gcattagagc
     1261 aaaatagaaa tgtttatgtt ttacctggat ctatgtttaa tcctatgaca aaaggtaatt
     1321 tattacgtat ccaagaaggt gctaaggtag tattaaacgc taatgatata tttgaagact
     1381 actatattta aaactaaaaa taacgagttg cttcatatt
//
LOCUS       DANBWH010000344         1417 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.355, whole genome shotgun sequence.
ACCESSION   DANBWH010000344 DANBWH010000000
VERSION     DANBWH010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.355"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            99..965
                     /gene="galU"
                     /locus_tag="PPY92_002763"
     CDS             99..965
                     /gene="galU"
                     /locus_tag="PPY92_002763"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDJ2626688.1"
                     /translation="MKKIKKAIIPAAGLGTRFLPATKAMPKEMLPILDKPTIQYIVEE
                     AARAGIEDIIIVTGRHKRAIEDHFDSQKELEMVLKEKGKSELLEKVQYSTELANIFYV
                     RQKEQKGLGHAISSARQFIGNEPFAVLLGDDIVESEVPAVKQLIDVYEETGHSVIGVQ
                     EVPEADTHRYGIIDPLTKNGRQYEVKKFVEKPAQGTAPSNLAIMGRYVLTPEIFDYLK
                     TQKEGAGNEIQLTDAIERMNNDNQVYAYDFEGERYDVGEKLGFVKTTIEYALKDDSMR
                     EELTRFIKALGL"
     gene            complement(1176..>1417)
                     /gene="sarU"
                     /locus_tag="PPY92_002764"
     CDS             complement(1176..>1417)
                     /gene="sarU"
                     /locus_tag="PPY92_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarU"
                     /protein_id="HDJ2626689.1"
                     /translation="SSRKNEILNLKDLFESIRFMYPQIVRSVNRLNNKGMLIKERSLA
                     DERIVLIKINKIQYNTIKSIFTDTSKILKPRKFFF"
BASE COUNT          525 a          183 c          268 g          441 t
ORIGIN      
        1 ataattacat actcaatgac actatgaagg cgttctaatt agtgttaaaa tgacgttgat
       61 acatagattt aatacttagg aaaaggagca cattaacttt gaaaaaaata aaaaaggcaa
      121 tcattcccgc tgctggttta gggactagat ttttaccagc aactaaagcg atgccaaagg
      181 aaatgcttcc tatcttagat aaacccacaa tacaatatat cgttgaagaa gctgcaagag
      241 ctggaattga agatattatt atagtgacag gtcgccacaa acgcgcgatt gaagatcatt
      301 ttgatagtca aaaagaatta gaaatggtgt taaaagaaaa aggtaaatct gaattactag
      361 aaaaagttca atactcgaca gaacttgcga atatttttta tgtaagacag aaagaacaaa
      421 aaggtttagg gcatgcgatt agttctgcgc gtcaatttat cggcaatgaa ccatttgcgg
      481 tattattagg tgacgatatt gttgaatctg aagtgccagc agtgaaacag ttgattgacg
      541 tttatgaaga aacggggcac tcagttatag gtgtccaaga agttccggaa gcagatactc
      601 atcgttatgg aattattgat ccattaacta aaaatggacg tcagtatgaa gtgaaaaagt
      661 ttgttgaaaa gccagcacaa ggtacggcac catcaaacct agctattatg ggccgatatg
      721 tattaacgcc tgaaattttc gattatttaa aaacgcaaaa agaaggcgca ggtaacgaaa
      781 ttcagttaac agatgcgatt gagcgtatga acaatgataa tcaagtgtat gcatatgact
      841 tcgaaggcga acgttatgac gttggagaaa aattaggatt tgtcaaaaca acaatagaat
      901 atgcgttgaa agatgacagt atgcgagaag aattaacacg atttattaaa gcattaggtt
      961 tataatatta aaagcatgat actcaataaa attggaacac gaaatggtga actaaattga
     1021 atgagtgtca tgcttaattt atgtgttgtg atatatagta gggtattgaa ataatataaa
     1081 aacaaaagaa agttaattat gtatttttat ttatactaaa ttgagaagta ttatgattgc
     1141 ttatttattt tatcaagagg taaatctgtt taaatttaaa agaaaaattt tcttggtttg
     1201 agaatcttgg aagtatctgt gaatatgctt ttaatagtgt tatattgtat tttatttatt
     1261 ttgattaaca caatcctttc atctgcaagg gatcgttctt tgattagcat acctttatta
     1321 tttaatctat taactgacct aacaatttga ggatacataa atctaatact ttcaaacaaa
     1381 tcttttaaat ttagtatctc attttttcga gatgata
//
LOCUS       DANBWH010000345         1399 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.356, whole genome shotgun sequence.
ACCESSION   DANBWH010000345 DANBWH010000000
VERSION     DANBWH010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.356"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..135
                     /locus_tag="PPY92_002765"
     CDS             <1..135
                     /locus_tag="PPY92_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDJ2626690.1"
                     /translation="RVAQLVSILLILISISLIVYRRIKYNPPLYSKVGALPWPTKKVK
                     "
     gene            143..628
                     /locus_tag="PPY92_002766"
     CDS             143..628
                     /locus_tag="PPY92_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDJ2626691.1"
                     /translation="MRKFLSKTHHHTNPLWRVYRLVKFSKVFKNVIIIEFSKFIPSMV
                     LKRHIYKQLLNINIGNQSSIAYKVMLDIFYPELITIGSNSVIGYNVTILTHEALVDEF
                     RYGPVTIGSNTLIGANATILPGITIGDNVKVAAGTVVSKDIPDNGFAYGNPMYIKMIR
                     R"
     gene            636..>1399
                     /locus_tag="PPY92_002767"
     CDS             636..>1399
                     /locus_tag="PPY92_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDJ2626692.1"
                     /translation="MAQKNNNVIPMTFDDAFYRKMAKQKFKQREYKRAAEYFEKVLEL
                     SPDDLEIQIDYAQCLVQLGIAKKAEHLFYDNIIYNRHLEDSFYELSQLNIEVNEPNKA
                     FLFGINYVIVSDDQDYRDELDQMFDVKYQSEEQIELEAQLFVVQILFQYLFSQGRLKD
                     AKNYVLHQPQEVQDHRVVRNLLAMCYLYLGEYDTAKALYEALLQEDSTDIYALCHYTL
                     LLYNTKENEQYQKYLKILNKVVPMNDDESFKLGIVL"
BASE COUNT          512 a          179 c          244 g          464 t
ORIGIN      
        1 agagttgcac aattagtatc aattctttta attttaataa gtataagttt aattgtatat
       61 agaaggatta agtataatcc accgttgtat agcaaagttg gggcgcttcc atggccaaca
      121 aaaaaagtga agtagtgata ttttgagaaa atttttatca aaaacacatc atcatacaaa
      181 ccctttatgg cgtgtatacc gtcttgttaa attttcgaaa gtttttaaga atgtaattat
      241 cattgaattt tcgaaattta ttccaagtat ggtactgaaa agacatatat ataaacaact
      301 tttaaatatt aatatcggta atcaatcatc gatagcttat aaagtaatgt tagatatttt
      361 ttacccagaa ctgattacga ttggtagtaa cagtgttatt ggttacaatg taacaatttt
      421 gacgcatgaa gcattagttg atgaatttcg ttatggacca gtgacgatag gatctaacac
      481 tttgattggt gcaaatgcta ccattttacc cggtataacg attggtgaca atgtaaaagt
      541 tgcagctggt acggttgttt caaaagatat accggataat ggatttgcat atggcaaccc
      601 tatgtatata aaaatgatta ggaggtgaca attttatggc gcaaaagaat aataatgtaa
      661 ttccaatgac ttttgatgat gcattttatc gtaaaatggc taaacagaag tttaaacaaa
      721 gagaatataa acgagctgct gaatactttg aaaaagtgtt agaattgtca cctgatgatc
      781 tggaaattca aattgattat gcacaatgtc tagtgcaact tggtattgct aaaaaagcag
      841 aacatttatt ttatgacaat attatttata ataggcatct agaagatagc ttttatgaat
      901 tgagtcagct caacattgaa gttaacgaac caaacaaggc attcttgttt ggtattaatt
      961 atgttattgt tagcgacgac caagattata gagatgaatt agatcaaatg tttgatgtga
     1021 aatatcaaag cgaagaacaa attgaacttg aagctcaatt gtttgtagtt caaatactat
     1081 tccaatatct tttttctcaa ggtcgattaa aagatgcaaa gaattatgtc ttacatcaac
     1141 cacaagaagt tcaagatcat cgtgtcgtac gtaatttatt ggcaatgtgt tatttatatc
     1201 tcggtgaata tgatacggct aaagcattgt acgaagcact attacaagag gatagtacag
     1261 atatatatgc attatgccat tatactttgc tactttataa cactaaggaa aatgaacaat
     1321 atcaaaaata tttaaaaata ttaaacaaag ttgtacctat gaatgacgat gaaagtttta
     1381 aattaggtat tgtattaag
//
LOCUS       DANBWH010000346         1386 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.357, whole genome shotgun sequence.
ACCESSION   DANBWH010000346 DANBWH010000000
VERSION     DANBWH010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.357"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            20..1336
                     /locus_tag="PPY92_002768"
     CDS             20..1336
                     /locus_tag="PPY92_002768"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HDJ2626693.1"
                     /translation="MPKIVVVGAVAGGATCASQIRRLDKESDIIIFEKDRDMSFANCA
                     LPYVIGEVVEDRKYALAYTPEKFYDRKQITVKTYHEVIAINDERQTVTVLNRKTNEQF
                     EESYDKLILSPGASANSLGFESDITFTLRNLEDTDAIDQFIKANQVDKVLVIGAGYVS
                     LEVLENLYERGLHPTLIHRSDKINKLMDADMNQPILDELDKREIPYRLNEEIDAINGN
                     EITFKSGKVEHYDMIIEGVGTHPNSKFIESSNIKLDRKGFIPVNDKFETNVPNIYAIG
                     DIATSHYRHVDLPASVPLAWGAHRAASIVAEQIAGNDTIEFKGFLGNNIVKFFDYTFA
                     SVGVKPNELKQFDYKMVEVTQGAHANYYPGNSPLHLRVYYDTSNRQILRAAAVGKEGA
                     DKRIDVLSMAMMNQLTVDELTEFEVAYAPPYSHPKDLINMIGYKAK"
BASE COUNT          489 a          221 c          267 g          409 t
ORIGIN      
        1 caataggagg taagttacga tgcccaaaat agtcgtagtc ggagcagtcg ctggcggtgc
       61 aacatgtgcc agccaaattc gacgtttaga taaagaaagt gacattatta tttttgaaaa
      121 agatcgtgat atgagctttg ctaattgtgc attgccttat gtcattggcg aagttgttga
      181 agatagaaaa tatgctttag cgtatacacc tgaaaaattt tatgatagaa agcaaattac
      241 agtaaaaact tatcatgaag ttattgcaat caatgatgaa agacaaactg taactgtatt
      301 aaatagaaag accaacgaac aatttgaaga atcttacgat aaactcattt taagccctgg
      361 tgcaagtgca aatagccttg gctttgaaag tgatattaca tttacactta gaaatttaga
      421 agacactgat gctatcgatc aattcatcaa agcaaatcaa gttgataaag tattggttat
      481 aggtgcaggg tatgtttcat tagaagttct tgaaaatctt tacgaacgtg gtttacaccc
      541 tactttaatt catcgatctg ataagataaa taaattaatg gatgctgaca tgaatcaacc
      601 tatacttgat gaattagata agcgggagat tccataccgt ttaaatgagg aaattgatgc
      661 tatcaatgga aatgaaatta catttaaatc aggaaaagtt gaacattacg atatgattat
      721 tgaaggtgtc ggtactcacc ccaattcaaa atttatcgaa agttcaaata tcaaacttga
      781 tcgaaaaggt ttcataccgg taaacgataa atttgaaaca aatgttccaa acatttatgc
      841 aataggcgat attgcaacat cacattatcg acatgtcgat ctaccggcta gtgttccttt
      901 agcttggggc gctcaccgtg cagcaagtat tgttgccgaa caaattgctg gaaatgacac
      961 tattgaattc aaaggcttct taggcaacaa tattgtgaag ttctttgatt atacatttgc
     1021 gagtgtcggc gttaaaccaa atgaactaaa gcaatttgac tataaaatgg tggaagtcac
     1081 tcaaggtgca cacgcgaatt attacccagg aaattcccct ttacacttaa gggtatatta
     1141 tgacacttca aaccgtcaga ttttaagagc agctgcagta ggaaaagaag gtgcagataa
     1201 acgtattgat gtactatcga tggcaatgat gaaccagcta actgtagatg agttaactga
     1261 gtttgaagtg gcttatgcac caccatatag ccaccctaaa gatttaatca atatgattgg
     1321 ttacaaagct aaataaatga atcatgcgat tcaatctaat cttaattgtc taggttagaa
     1381 ttattt
//
LOCUS       DANBWH010000347         1385 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.358, whole genome shotgun sequence.
ACCESSION   DANBWH010000347 DANBWH010000000
VERSION     DANBWH010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.358"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            543..1172
                     /locus_tag="PPY92_002769"
     CDS             543..1172
                     /locus_tag="PPY92_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4887 domain-containing protein"
                     /protein_id="HDJ2626694.1"
                     /translation="MSNQNYDYNKNEDGSKKKMSTTAKVVSIATVLLLLGGLVFAIFA
                     YVDHSNKAKERMLNEQKQEQKEKRQKENAEKERKKKQQEEKEQNELDSQANQYQQLPQ
                     QNQYQYVPPQQQAPTKQRPAKEENDDKASKDESKDKDDNASQDKSDDNQKKTDDNKQP
                     AQPKPQPQQPTPKPNNNQQNNQSNQQAKPQAPQQNSQSTTNKQNNANDK"
BASE COUNT          582 a          196 c          234 g          373 t
ORIGIN      
        1 acgaataaac atctcttaaa agtatgtgta aaacgctgca tgatacaaac gaaggtaaaa
       61 atttgactcc ctttagtagt ggacccgtac gttaatcgtg cgggtcgttt ttatttattt
      121 cttattccca ttatacatca atttaaagca ttaatttttt aaacaaattt aagaatacat
      181 agtaatataa caatctaaac ataaaaactt ttaacacaac acttaaacca atgctttaat
      241 tttcaatacg tagctataat ttgttgtaaa atcaaaaagg ttaaaatgtt aattttcaaa
      301 aaaaggctca aaatatgttt gatttagtta ttaaatgtta agatatataa gactactatt
      361 tctttgtaaa aatgaatccg atttacgagt gagtaatagt gaaggcagtt ttaagttgaa
      421 gaaggcaaaa agagtaaatg tttattaata tttgtagaaa ctaggtaagc aaattagttg
      481 tgaaaatgtt aatggttgcg tgataatttc tatatttaaa ttagtttgaa gtgagggaga
      541 gtatgtcgaa tcaaaattac gactacaata aaaatgaaga tggaagtaag aagaaaatga
      601 gtacaacagc gaaagtagtt agcattgcga cggtattgct attactcgga ggattagtat
      661 ttgcaatttt tgcatatgta gatcattcga ataaagctaa agaacgtatg ttgaacgaac
      721 aaaagcagga acaaaaagaa aagcgtcaaa aagaaaatgc agaaaaagag agaaagaaaa
      781 agcaacaaga ggaaaaagag cagaatgagc tagattcaca agcaaaccaa tatcagcaat
      841 tgccacagca gaatcaatat caatatgtgc cacctcagca acaagcacct acaaagcaac
      901 gtcctgctaa agaagagaat gatgataaag catcaaagga tgagtcgaaa gataaggatg
      961 acaatgcatc tcaagataaa tcagatgata atcagaagaa aactgatgat aataaacaac
     1021 cagctcagcc taaaccacag ccgcaacaac caacaccaaa gccaaataat aatcaacaaa
     1081 ataatcaatc aaatcagcaa gcaaaaccac aagcaccaca acaaaatagc caatcaacaa
     1141 caaataaaca aaataatgct aatgataagt agtatttagt caaacaaaaa tgaaccagta
     1201 tgacagacaa cacaattaat taggttgtct cgaatattgg ttcttatttt tataattgtt
     1261 aattagggga gagatgatac ttaaatagtt agttgtttat tttacggata gtgaaattta
     1321 ttttgagtga ggtgggacag aaatgatatt ttcgcaaaat ttatttcgtc gtctgacccc
     1381 aactt
//
LOCUS       DANBWH010000348         1372 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.359, whole genome shotgun sequence.
ACCESSION   DANBWH010000348 DANBWH010000000
VERSION     DANBWH010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.359"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..790
                     /locus_tag="PPY92_002770"
     CDS             <1..790
                     /locus_tag="PPY92_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FKLRK protein"
                     /protein_id="HDJ2626695.1"
                     /translation="AKGGISKEDTKASSDGVWMPINEKGYFDFDFKKNPFDNLELKKN
                     DEISLTFAPDDEDEALKSLIFKTKVTSLEDIDKAETKYDHTKVEKVKVLKDVKEDIHV
                     DEIYGSLYHTEKGKGILDKQGTKEITGKTKFANAVVKVYSDLGDAQLFPDIQVDENGK
                     FSFDAEKAGFRLQNGETLNFAVVKPITGDLLHQGFVSKYIDVYESPEEKKEREFEEKL
                     ENTPAYHKLHGDKIVGYDVQGNPSTWFYPLGEKKVERTTPKLEK"
     gene            complement(899..1207)
                     /gene="spn"
                     /locus_tag="PPY92_002771"
     CDS             complement(899..1207)
                     /gene="spn"
                     /locus_tag="PPY92_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myeloperoxidase inhibitor SPIN"
                     /protein_id="HDJ2626696.1"
                     /translation="MKFKKVLVATAMVGVLATGVVGYGNQADAKVYSQNGLVLHDDAN
                     FLEHELSYIDVLLDKNADQATKDNLRSYFADKGLHSIKDIINKAKQDGFDVSKYEHVK
                     "
BASE COUNT          509 a          185 c          232 g          446 t
ORIGIN      
        1 agctaaaggt ggaattagta aagaagatac taaagctagt tccgatggtg tttggatgcc
       61 tattaatgaa aaaggatatt ttgattttga tttcaaaaaa aatccttttg ataacttaga
      121 gttaaagaaa aatgatgaaa tctcattaac atttgcacct gatgatgaag atgaagcatt
      181 gaagtcatta attttcaaaa ctaaagtaac gagcttagaa gatatcgata aagcagaaac
      241 aaaatatgac catactaaag tggaaaaagt aaaagtattg aaagatgtta aagaagatat
      301 acatgtagat gaaatttacg gaagcttata tcatacagaa aaaggtaaag gtattcttga
      361 taaacaagga actaaagaga ttacagggaa gactaaattc gcgaatgcag tagtgaaagt
      421 atattctgac ttaggtgatg cgcaattgtt ccctgatatt caagtagatg aaaatggtaa
      481 atttagcttt gatgctgaaa aagctggttt cagattgcaa aatggagaaa cattgaattt
      541 tgcagtagtt aaacctatta ctggtgacct attacatcaa ggatttgtct ctaaatatat
      601 tgatgtatat gaatctccgg aagaaaagaa agaacgtgaa tttgaagaaa aacttgaaaa
      661 cacgcctgca tatcataaat tacatggtga taaaattgta ggatatgatg ttcaaggtaa
      721 tccatcaact tggttctatc cattaggtga aaagaaagtt gaacgtacaa cacctaaatt
      781 agaaaaataa ttaataaaat aaaacagctt aatgatgtaa tgaaattagt gagttaatca
      841 ctgacttcaa cgtcattgag ctgtttttta tttgcttcgt tacaaagcat tattgaattt
      901 attttacgtg ttcatatttt gaaacatcaa agccgtcttg cttagctttg ttgataatgt
      961 ctttgattga atgtagtcct ttatcggcga agtatgatct taagttgtct tttgtagctt
     1021 ggtcagcatt cttatctaat aacacatcga tatagcttaa ttcatgttct aagaagtttg
     1081 cgtcatcatg tagtacgagt ccattttgag aataaacttt cgcatctgct tgattaccat
     1141 atccaacaac gccagttgct aatacaccta ccattgccgt agctactaaa acctttttaa
     1201 atttcatatc tatcactcct ctaaaaattg taactctatc ataacactga atattaagaa
     1261 aattacgttt attaagtcga tttaataatt tttaataaat agttaaactg acaaatattg
     1321 cttaaatgca attaatcttt aaaacggtgt ttatggattt ttcctactaa aa
//
LOCUS       DANBWH010000349         1350 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.360, whole genome shotgun sequence.
ACCESSION   DANBWH010000349 DANBWH010000000
VERSION     DANBWH010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.360"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            3..584
                     /locus_tag="PPY92_002772"
     CDS             3..584
                     /locus_tag="PPY92_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HDJ2626697.1"
                     /translation="MNTKFLGKTLVASALVLTTLGTGLHSSYLGLNTNKVVKTAKAEE
                     KMTNGQLWKKVKDSLIDSNIISGNENEEITVTHVNKTGYSSSVSAYGNNNDDFSSTPS
                     NFSKLKEIDLKKDNVPSDDFNTTVSGEDSWKTLTSKLKEKGLVTDGQTVTIHCNDKSD
                     NTKSSVSGKVGADLTSGNGTTFKKRFIDKITID"
     gene            complement(1057..1335)
                     /locus_tag="PPY92_002773"
                     /pseudo
     CDS             complement(1057..1335)
                     /locus_tag="PPY92_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500445.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BsaG protein"
BASE COUNT          525 a          225 c          173 g          427 t
ORIGIN      
        1 aaatgaatac aaaattttta ggtaaaacat tagtagcaag tgctttagta ttaacaacat
       61 taggaacagg tttacattct tcatacttag gattaaatac aaataaagtt gttaaaacag
      121 caaaagcaga agaaaaaatg acaaatggtc aattgtggaa aaaagttaaa gattcattaa
      181 ttgattcaaa tattattagt ggtaacgaaa atgaagagat tacagtaaca catgttaata
      241 aaaccggata ttcttcaagt gtttctgcct atggtaataa taatgacgat ttttcaagta
      301 ctccaagtaa tttttctaaa cttaaggaaa ttgacttgaa aaaagataat gtaccgtcag
      361 atgattttaa tactactgtt agtggtgaag atagttggaa aacacttaca tctaaattaa
      421 aggaaaaagg tttggttaca gatggacaaa cagtaactat tcattgtaat gataagtctg
      481 acaatacaaa atcatctgtt tcaggtaaag taggagcaga tctgacaagt ggcaatggaa
      541 ctacattcaa aaaacgcttt attgataaaa tcacaattga ttaaacctta aactcaaatt
      601 tccttaattc ataaaacctt aataactaac cctttcttac aaaccaccta gcccataata
      661 tattaaaacg tcatttttat ttcaattagc ggaaaataaa aaaaccattc cgcattaact
      721 ttggcaagtt ggaatggtta aaaaatattt tagaagccag cgtttggctt gctttgacga
      781 catgttcgcg catgtcgtct tatttttaca tttatataat aacacgcaat tcaataaaat
      841 gtctaatcaa taaacctcaa ttacattcaa attcaaaaac tttgcaccta atattcatcg
      901 cttaaataaa cattacgttt aattaaccag acattgtcaa tcgcgtctaa tacccctgct
      961 gtccgaatac aaaattattt tgtattcata tttatctgtt ctctttcctc ccttttaaac
     1021 gcaactaaat ttcttttacc attagcgccc caatctttaa tctttaattg ttctaccgct
     1081 ccatttatta aatcctttaa agagtaaaac tgctaatagc aacgtgataa taatatagat
     1141 cgccaatgtt aatgtaactg gtatactccc ttcgataaac atataaacgt aacacgtagc
     1201 atatgtgatt ggtaaataga accacgaatg atctccaagc acttctaatc caaaataaac
     1261 gttaaaaata aacattaaaa ctccgacaac aatagccatt acatctttaa tgaaaaatat
     1321 atctaacatt atcacaaatt attaataatg
//
LOCUS       DANBWH010000350         1345 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.361, whole genome shotgun sequence.
ACCESSION   DANBWH010000350 DANBWH010000000
VERSION     DANBWH010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.361"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..1152
                     /gene="scpA"
                     /locus_tag="PPY92_002774"
     CDS             <1..1152
                     /gene="scpA"
                     /locus_tag="PPY92_002774"
                     /EC_number="3.4.22.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain A"
                     /protein_id="HDJ2626698.1"
                     /translation="PKLIALSLIFSLSVTPIANAESNSNIKAKDKKHVQVNVEDKSVP
                     TDVRNLAQKDYLSYVTSLDKIYNKEKASYTLGEPFKIYKFNKKSDGNYYFPVLNTEGN
                     IDYIVTISPKITKYSSSSSKYTINVSPFLSKVLNQYKDQQITILTNSKGYYVVTQNHK
                     AKLVLKTPRLEDKKLKKTESIPTGNNVTQLKQKASVTMPTSQFKSNNYTYNEQYINKL
                     ENFKIRETQGNNGWCAGYTMSALLNATYNTNKYHAEAVMRFLHPNLQGQRFQFTGLTP
                     REMIYFGQTQGRSPQLLNRMTTYNEVDNLTKNNKGIAVLGSRVESRNGMHAGHAMAVV
                     GNAKLDNGQEVIIIWNPWDNGFMTQDAKNNVIPVSNGDHYRWYSSIYGY"
     gene            1183..>1345
                     /locus_tag="PPY92_002775"
     CDS             1183..>1345
                     /locus_tag="PPY92_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin A"
                     /protein_id="HDJ2626699.1"
                     /translation="MEQIELFSIDKFKCNSEAKYYLNIIEGEWHPQDLNDSPLKFILS
                     TSDDSDYICK"
BASE COUNT          516 a          198 c          214 g          417 t
ORIGIN      
        1 ccaaaattaa tagctttaag tttaatattt tctttaagtg ttacccctat tgcaaacgct
       61 gagagcaatt caaatatcaa agcaaaagat aagaagcatg tgcaagttaa tgtcgaggac
      121 aagagtgttc ctactgacgt acgtaattta gcgcaaaaag actatctttc ttatgttact
      181 tcacttgata aaatctataa taaagagaaa gcctcgtata cacttggtga accttttaaa
      241 atttataaat tcaacaaaaa gagtgatggt aattattatt ttccagtttt gaacactgaa
      301 ggtaatatcg attatatagt tacaatttca ccaaaaatca ctaagtattc tagttcatct
      361 tctaaataca caatcaatgt ttctccattt ttatctaaag tcttaaatca atataaagat
      421 caacaaatta ctattttgac aaactcaaaa ggttactatg tggttactca aaatcataaa
      481 gctaaactag tactaaaaac accacgtcta gaagataaaa agttaaaaaa gactgagagt
      541 atacctactg gcaataatgt aacgcaacta aagcaaaaag cttctgtcac tatgccaact
      601 agtcaattta aaagtaataa ttatacttac aacgagcaat atataaataa acttgagaat
      661 ttcaagataa gagaaactca aggtaacaat ggttggtgtg caggctatac gatgtctgca
      721 ttactaaatg cgacttataa tactaacaaa tatcatgcag aagctgtgat gagattttta
      781 cacccaaact tacaaggaca acgattccaa tttactggtt taacgccaag agagatgatt
      841 tattttggac aaactcaggg aagaagtcca caacttctta atagaatgac tacatataat
      901 gaagttgaca atcttactaa aaataataaa ggaattgctg ttttaggtag tagagtagaa
      961 tcaagaaatg gtatgcacgc agggcatgcc atggcagttg ttgggaacgc taaattagat
     1021 aatggacaag aggttataat tatatggaat ccatgggata atggttttat gactcaagat
     1081 gctaaaaaca atgttattcc agtatccaat ggcgaccatt atcgatggta ttcatctatt
     1141 tatggttatt aaaatttgta ttttagaaag gtaaataata ttatggaaca aattgaatta
     1201 tttagtattg ataaattcaa atgtaattca gaggctaagt attatcttaa tattattgag
     1261 ggagaatggc atcctcaaga tttaaatgat agtcctttaa aatttattct cagtacctca
     1321 gacgattctg attacatttg caaat
//
LOCUS       DANBWH010000351         1306 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.362, whole genome shotgun sequence.
ACCESSION   DANBWH010000351 DANBWH010000000
VERSION     DANBWH010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.362"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(112..612)
                     /locus_tag="PPY92_002776"
     CDS             complement(112..612)
                     /locus_tag="PPY92_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDJ2626700.1"
                     /translation="MKKLVTATTLTAGIGTALVGQAHHADAAENYTNYNNYNYNTTQT
                     TTTTTTTTTTSSISHSGNLYTAGQCTWYVYDKVGGEIGSTWGNANNWAAAAQGAGFTV
                     NHTPSKGAILQSSEGPFGHVAYVESVNSDGSVTISEMNYSGGPFSVSSRTISASEAGN
                     YNYIHI"
     gene            complement(849..1271)
                     /locus_tag="PPY92_002777"
     CDS             complement(849..1271)
                     /locus_tag="PPY92_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HDJ2626701.1"
                     /translation="MTTEMNDVTNHQPNRKSNDENIMAMLIYLLSLFTSIIGPLIIWL
                     LKKDESKLVDRAGKNYLNYTISYIIWSIVLVVITLIGVFLIATDISFIIIIGFIITFI
                     GILSIFAFSILSFVFTIIACVKYYNGQEYVIPLTIRFI"
BASE COUNT          448 a          193 c          215 g          450 t
ORIGIN      
        1 caacgaaaaa tagcaaaaaa agagcttctc ctataaagga aagctcaaag ttttttgatg
       61 acatatgtac tagaattaag tttcaagaca atatgtatca tcgtgtttat attaaatatg
      121 gatgtagttg tagttacctg cttcacttgc agaaatagtt ctagaactta ctgagaaagg
      181 tccgccacta taattcattt ctgaaattgt aactgaacca tcactgttta cactttctac
      241 atatgcaacg tgaccaaatg gtccttcaga agattgtagg atagcgcctt tagaaggtgt
      301 atgatttact gtgaatccag caccttgtgc agcagcagcc caattattag catttcccca
      361 agtagaaccg atttctccgc ctactttatc atatacatac caagtacatt gtcctgcagt
      421 gtataagtta ccagaatgtg aaattgatga tgtagttgtc gtagttgttg tagtcgttgt
      481 agtttgagtc gtgttgtagt tatagttgtt gtaatttgta taattttcag cagcatctgc
      541 atgatgtgct tgacctacta atgctgtacc gattcctgct gttaacgtag ttgctgttac
      601 taattttttc atgaataaag tcctccaaag ttctatatct ttttttataa ataaaacgta
      661 gcgactgttt tattctcaca tctcgaattg atgacaatag ttactttaac aaaattaatg
      721 cttcttgtgg ggaatgttat tgatttgtaa aagaataaaa aaactttgac taattttgta
      781 ataaaaatta gtcaaagtta caatgagatt aacagataat taataggaaa tatttatttg
      841 taatatgttt aaataaatcg aattgttaaa ggtattacat attcttggcc attataatat
      901 ttgacacacg caataattgt gaatacaaaa gataatattg agaaagcgaa tatggataaa
      961 ataccgataa atgtaatgat gaaacctata atgataatga aactaatatc ggtagcaatt
     1021 aggaaaacgc ctattaaagt gataacgact aaaacgatag accaaataat ataagaaatc
     1081 gtatagttaa gataattttt tccagcacga tcaactagtt tcgattcatc ttttttcaat
     1141 aaccatatta tcagtggacc aataatagat gtgaataaac ttaataaata gataagcatc
     1201 gccataatgt tctcatcatt ggatttgcga ttcggttgat gatttgttac gtcgttcatt
     1261 tcagttgtca tattagacac tcctttgaaa attgtaatat tatctt
//
LOCUS       DANBWH010000352         1285 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.363, whole genome shotgun sequence.
ACCESSION   DANBWH010000352 DANBWH010000000
VERSION     DANBWH010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.363"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(139..1164)
                     /locus_tag="PPY92_002778"
     CDS             complement(139..1164)
                     /locus_tag="PPY92_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HDJ2626702.1"
                     /translation="MISFENDYLEGAHEKVLKRLVDTNLVQASGYGFDQFTAQAIEKI
                     KDTIDCPNATIRFLVGGTQTNQVVINSMLESYEGVISADTGHVAVHEGGAIEYSGHKV
                     ITIPSKEGKVSASDVETYMETFKSDFKKDHMVFPGMVYISHPTEYGTLYSKSELEELC
                     KVCKQYQLPLFMDGARLGYGLMSDQSDMTIKDIAKYCDVFYIGGTKIGALCGEAIVFT
                     KNNEPKQFTTRIKHHGALLAKGRLTGIQFLELFTDNLYFNISRHAIEMANKMKDGFKN
                     KGYRLYFDSPTNQQFFILSNEKIAELEQKVKFAVWEKYDDQHRVVRFATSWATTEENL
                     NKLLELI"
BASE COUNT          407 a          239 c          175 g          464 t
ORIGIN      
        1 gtagtatctt gttttggaat aatgtaattt tatatcatca acggcttcaa atgagtagtt
       61 tcatataaat caataaacac aactactcaa ctgatcttaa ttaataaatg atttgtcaga
      121 tgacacataa cagcattatt agattaattc taatagtttg tttaaatttt cttcggttgt
      181 cgcccagctg gttgcgaatc taacaacacg atgttgatca tcgtattttt cccaaacagc
      241 aaatttaact ttttgttcta actctgctat tttctcgtta cttaaaataa aaaattgttg
      301 attggttgga gaatcaaagt aaagacgata gcctttattt ttaaacccgt ctttcatctt
      361 atttgccatt tcgatagcat gtctgcttat attaaaatat aaattgtccg taaataattc
      421 taaaaattgt atgcctgtta accgtccttt tgctaaaagg gcaccgtgat gtttgattcg
      481 agtggtaaat tgtttcggtt cattattttt cgtaaaaaca atggcttccc cgcataatgc
      541 acctatcttc gtaccaccta tataaaatac atcacaatat ttagcgatgt ctttaatagt
      601 catatctgat tgatcactca tcaatccata ccctaatcgt gcaccatcca taaataatgg
      661 aagctgatat tgcttacata ctttgcataa ctcttccaat tctgatttag agtataaagt
      721 gccatattct gtaggatgag aaatatatac cattcctggg aataccatat ggtccttttt
      781 aaaatcactt ttaaatgtct ccatgtaagt ttcaacatct gaagcactaa cttttccttc
      841 cttagagggt atagtaatta ctttatgtcc actatattca attgcaccac cctcatgcac
      901 agcaacatga ccagtgtctg ctgaaatgac cccttcgtaa ctttctaaca ttgaattaat
      961 aacaacctga ttggtttgtg ttccacctac taaaaaacga attgtagcat ttggacaatc
     1021 aattgtatct ttaatctttt caattgcctg agctgtgaat tgatcaaagc catatcccga
     1081 agcttgtaca agatttgtat ctactaatcg ttttaatact ttttcatgag caccttctaa
     1141 ataatcattt tcaaatgaga tcactacatt tccccctaaa actaatatca gcattttaat
     1201 atgataaact aatttcaaaa ctagttcgat atttaaaatg tattgtgaat tgttaaagtt
     1261 tgtatcgcat tatcgcgaag ttgaa
//
LOCUS       DANBWH010000353         1262 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.364, whole genome shotgun sequence.
ACCESSION   DANBWH010000353 DANBWH010000000
VERSION     DANBWH010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.364"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            721..>1262
                     /locus_tag="PPY92_002779"
     CDS             721..>1262
                     /locus_tag="PPY92_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002494594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626703.1"
                     /translation="MNLRGHENRLKFHAKYDVTPISHLKLLEGQKKDGEGGILTDSYY
                     CFSYSLKGNSKKVLGTFNCGYHIAEDLLKLSNQDKLPLFNPFKVINEGNQLQGVTNKG
                     NLNINRQRKQYNEVALQLSNAINLIIICYEDNIKEPLSTIKYETDKYYYSEPYDSKIK
                     AVNTIIGNLFDKKLVEKISE"
BASE COUNT          511 a          129 c          214 g          408 t
ORIGIN      
        1 aaaattcgat actattccat aatgatggaa tgtgatgtat atgattaatc aatatacatc
       61 acatttcatt taatgcaact attgcacaag ttaatagtat atttaaaaat aggttagtga
      121 gattgattaa ttattgatta tgttggtatc aatgaaagct tggaagaagg gcttaaaaat
      181 ataaaaactt ggataaatca caatacaatc atagacacag ataagttcgt caatctgtta
      241 atgaattatg atgtgataga gttaagtgag gataaatgtt aacgttttat taaaacagtc
      301 acagaattag atagagcatt tgcaatttgt gctactgaac ttataatcta agagctcaat
      361 agtgaaattg tcttttttaa agttgttaaa gcataaattt gttgaattat tacagaaaaa
      421 gatcgaaatt tagttactgc taatggaacg gcagcattag agtttgccga actcatttta
      481 aaaggacttg aatttgatag taaagaaaat attgaaaggc aaatctatat gtatcaaaga
      541 ggcttctatg agtattgtaa taagtatggc aatccttatc aataattaaa aaccgcatca
      601 ttatctgata cgcagaagcg tatcataagt gatggtaaaa aatatgagta agtagatgaa
      661 gagtgaaaat cagattaatt aataataatg tatcaaattt aaataaaggg gtttttaagt
      721 atgaatttaa gaggtcatga aaatagactt aaatttcatg cgaaatatga tgtgacacct
      781 atatcacatt taaaattatt agaaggtcaa aagaaagacg gtgaaggcgg catactgaca
      841 gatagctatt actgtttttc atacagctta aaaggtaatt ctaaaaaagt tttaggtacg
      901 tttaattgtg gttatcatat tgctgaagat ttactaaaat tatcaaatca agataaatta
      961 cctttattta acccgtttaa agtaattaat gaaggtaatc aattgcaggg cgtaacgaat
     1021 aaaggtaatt taaatattaa taggcaaaga aaacagtata atgaagtggc tttacagctt
     1081 tcaaatgcta ttaatttaat cataatttgt tatgaggata atattaaaga accactttca
     1141 acgataaaat acgaaaccga taaatattat tatagtgaac catatgatag taaaataaaa
     1201 gcagtaaata ctattatagg taatttgttt gataagaaat tagttgagaa gatatctgaa
     1261 at
//
LOCUS       DANBWH010000354         1242 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.365, whole genome shotgun sequence.
ACCESSION   DANBWH010000354 DANBWH010000000
VERSION     DANBWH010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.365"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            203..1162
                     /gene="hyl"
                     /locus_tag="PPY92_002780"
     CDS             203..1162
                     /gene="hyl"
                     /locus_tag="PPY92_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hemolysin"
                     /protein_id="HDJ2626704.1"
                     /translation="MKTRIVSSVTTTLLLGSILMNPVANAADSDINIKTGTTDIGSNT
                     TVKTGDLVTYDKENGMHKKVFYSFIDDKNHNKKLLVIRTKGTIAGQYRVYSEEGANKS
                     GLAWPSAFKVQLQLPDNEVAQISDYYPRNSIDTKEYMSTLTYGFNGNVTGDDTGKIGG
                     LIGANVSIGHTLKYVQPDFKTILESPTDKKVGWKVIFNNMVNQNWGPYDRDSWNPVYG
                     NQLFMKTRNGSMKAAENFLDPNKASSLLSSGFSPDFATVITMDRKASKQQTNIDVIYE
                     RVRDDYQLHWTSTNWKGTNTKDKWTDRSSERYKIDWEKEEMTN"
BASE COUNT          480 a          186 c          205 g          371 t
ORIGIN      
        1 ttcctttcaa attttaaata aaagttaaaa acatattctt aaaataatca ctcatcatca
       61 ctcagtaatt tatcagttgc tacattaaat atactaatat aaacgattat aaatatttga
      121 tatgtctcaa ctgcaatatt ctaaattgac atattgatta ttgtttcctc aaatcgttaa
      181 aaaaaataga aggatgatga aaatgaaaac acgtatagtc agctcagtaa caacaacact
      241 attgctaggt tccatattaa tgaatcctgt cgctaatgcc gcagattctg atattaatat
      301 taaaaccggt actacagata ttggaagcaa tactacagta aaaacaggtg atttagtcac
      361 ttatgataaa gaaaatggca tgcacaaaaa agtattttat agttttatcg atgataaaaa
      421 tcacaataaa aaactgctag ttattagaac gaaaggtacc attgctggtc aatatagagt
      481 ttatagcgaa gaaggtgcta acaaaagtgg tttagcctgg ccttcagcct ttaaggtaca
      541 gttgcaacta cctgataatg aagtagctca aatatctgat tactatccaa gaaattcgat
      601 tgatacaaaa gagtatatga gtactttaac ttatggattc aacggtaatg ttactggtga
      661 tgatacagga aaaattggcg gccttattgg tgcaaatgtt tcgattggtc atacactgaa
      721 atatgttcaa cctgatttca aaacaatttt agagagccca actgataaaa aagtaggctg
      781 gaaagtgata tttaacaata tggtgaatca aaattgggga ccatatgata gagattcttg
      841 gaacccggta tatggcaatc aacttttcat gaaaactaga aatggttcta tgaaagcagc
      901 agagaacttc cttgatccta acaaagcaag ttctctatta tcttcagggt tttcaccaga
      961 cttcgctaca gttattacta tggatagaaa agcatccaaa caacaaacaa atatagatgt
     1021 aatatacgaa cgagttcgtg atgactacca attgcattgg acttcaacaa attggaaagg
     1081 taccaatact aaagataaat ggacagatcg ttcttcagaa agatataaaa tcgattggga
     1141 aaaagaagaa atgacaaatt aatgtaaatt atttgttcat gtacaaataa atataattta
     1201 taactttagc cgataacttc agaaatgatg tttttcggct aa
//
LOCUS       DANBWH010000355         1236 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.366, whole genome shotgun sequence.
ACCESSION   DANBWH010000355 DANBWH010000000
VERSION     DANBWH010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.366"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            41..1159
                     /locus_tag="PPY92_002781"
     CDS             41..1159
                     /locus_tag="PPY92_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDJ2626705.1"
                     /translation="MNKQQSKVRYSIRKVSIGILSISIGMFLALGMSNKAYADEIDKS
                     KDFTRGYEQNVFAKSELNANKNTTKDKIKNEGAVKTSDTSLKLDNKSAISNGNEINQD
                     IKISNTPKNSSQGNNLVINNNEPTKEIKIANLEAQNSNQKKTNKVTNNYFGYYSFREA
                     PKTQIYTVKKGDTLSAIALKYKTTVSNIQNTNNIANPNLIFIGQKLKVPMTPLVEPKP
                     KTVSSNNKSNSNSSTLNYLKTLENRGWDFDGSYGWQCFDLVNVYWNHLYGHGLKGYGA
                     KDIPYANNFNSEAKIYHNTPTFKAEPGDLVVFSGRFGGGYGHTAIVLNGDYDGKLMKF
                     QSLDQNWNNGGWRKAEVAHKVVHNYENDMIFIRPFKKA"
BASE COUNT          521 a          151 c          208 g          356 t
ORIGIN      
        1 cgttataatg tttatatatt attgtaagga gtgttccatc atgaataaac aacaaagtaa
       61 agtacgctat tcaattagaa aagttagtat tggaattttg tcaatttcaa taggtatgtt
      121 tttggcattg ggtatgtcga acaaagcata tgcagatgaa attgataaat ctaaagattt
      181 tacaagaggg tatgagcaaa atgtattcgc gaaatcagag ttaaatgcta ataaaaatac
      241 gacaaaagac aaaataaaaa atgaaggtgc tgttaaaaca tcggacacaa gtttaaagtt
      301 agacaacaaa tcagcaattt caaacggaaa tgaaattaat caagatataa agatttcaaa
      361 tactccgaaa aactcaagcc aaggtaacaa tctagttatt aataacaatg aacctactaa
      421 agaaattaaa attgcaaact tggaagctca aaattctaat cagaagaaaa cgaataaagt
      481 tactaataat tactttggtt actacagttt tagagaagct ccaaaaacac aaatctatac
      541 tgtaaaaaaa ggagacacac ttagtgctat agcattaaaa tacaaaacta cagtttcaaa
      601 tattcaaaat acaaataata tagcaaatcc taatttaata tttattggtc aaaaattaaa
      661 agtgccaatg acaccattag tagaaccaaa accaaaaaca gtgtcttcaa ataataaaag
      721 taatagtaat agcagtacat taaattattt gaaaacatta gagaatagag gatgggattt
      781 cgacggtagt tatggatggc aatgtttcga tttagttaat gtatattgga atcatcttta
      841 tggtcatgga ttaaaaggat atggagctaa agatatacca tatgcaaata attttaatag
      901 tgaagctaaa atttatcaca acacaccaac tttcaaagct gaacctgggg acttagtggt
      961 ttttagtgga agatttggtg gaggatatgg tcatacagct attgtcttaa atggtgatta
     1021 tgatggaaaa ttaatgaagt tccaaagttt agatcaaaac tggaataatg gtggatggcg
     1081 taaagcagag gttgcacata aagttgttca taattatgaa aatgatatga tttttattag
     1141 accatttaaa aaagcataat ttaaatcgaa ggcaggacat tgaaatatga aattttcaac
     1201 tttaagtgaa gaagaattta ccaactacac caaaaa
//
LOCUS       DANBWH010000356         1222 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.367, whole genome shotgun sequence.
ACCESSION   DANBWH010000356 DANBWH010000000
VERSION     DANBWH010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.367"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            73..>1222
                     /locus_tag="PPY92_002782"
     CDS             73..>1222
                     /locus_tag="PPY92_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDJ2626706.1"
                     /translation="MGYRTLKSIFHEHNESKMKEEYTKRFNSLASFNTNINIIPMENG
                     KKVNDLEYPLFFMVTKNLSKKQELISINSRKIDRALNSLPYAAREQYFNDLLIDELQS
                     TNEIENVFSTKQEIAHALNNQASEFLKFRGLVDQYKEIELNKKIKVDNVRDIRAIYDK
                     LVSNEINEQDKLDGELFRKNFVGVHDGSTNKYIHVGLQPETKIVEYIGEMLTFLKYFD
                     APQPFKIMASHYLFEYIHPFYDGNGRVGRFIIAKLLSDYYDNYTALTFSYVINRNKSK
                     YYKAFMTASNHLNCGDLTEFIDTMLELLIAGQERILDELIPKMDATEKLTLYLTSHYK
                     QIDYEFLYLLSMDKLFGNKRNRLTLIDLENILGVGRVKINNTIKKYDNY"
BASE COUNT          491 a          131 c          202 g          398 t
ORIGIN      
        1 tatttagcta atctaatgaa aatggattta ttggacaaaa aatacatttt aaatgagtag
       61 aggaggctgc aaatgggtta cagaacttta aaaagtattt ttcatgaaca caatgaaagt
      121 aaaatgaagg aagagtatac taaaagattt aattctttag cttctttcaa tactaatatt
      181 aatatcatac ctatggaaaa tggaaaaaaa gttaatgatt tggaatatcc tctattcttt
      241 atggtgacta aaaatctatc aaaaaaacaa gaattaatat caattaatag tagaaaaatt
      301 gatagagcac ttaattcttt accatatgca gctagagaac aatattttaa tgatttatta
      361 atcgatgaat tacaaagtac taatgaaatt gaaaatgtat ttagtactaa acaagagatt
      421 gcacatgcgt taaataacca agcatcagaa tttcttaagt tcagaggcct cgtggatcaa
      481 tataaagaga tagaacttaa taaaaaaatt aaagttgata atgtaagaga cattagagcg
      541 atttatgata aattagtttc aaatgaaatt aacgaacaag ataagttaga tggagagcta
      601 tttcgtaaaa attttgtcgg tgtgcatgat gggtcaacga ataaatatat acatgttggg
      661 ttacaacctg aaaccaaaat tgttgaatat ataggtgaaa tgctaacatt tttaaaatat
      721 tttgatgcgc ctcagccgtt caaaatcatg gctagtcatt atctgtttga atatatacat
      781 cctttttatg atggcaatgg tagagttgga agatttataa ttgccaagct cttgagtgat
      841 tattatgata attatactgc tttgacattt tcatatgtta taaatagaaa taaatcgaaa
      901 tattacaagg cgtttatgac tgcttcaaat catttgaatt gtggtgattt gacggaattt
      961 attgacacaa tgcttgagct attgatagca ggacaagaaa gaatacttga tgagttaata
     1021 ccaaaaatgg atgcgactga aaaattaacg ctttatttaa ctagtcatta taaacagata
     1081 gattatgaat ttttgtatct cctttcaatg gataaattgt ttggaaataa aagaaataga
     1141 ttaacattaa ttgatttaga aaacatattg ggagtaggaa gagtaaaaat taataataca
     1201 attaaaaaat atgataacta ct
//
LOCUS       DANBWH010000357         1212 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.368, whole genome shotgun sequence.
ACCESSION   DANBWH010000357 DANBWH010000000
VERSION     DANBWH010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.368"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            137..457
                     /locus_tag="PPY92_002783"
     CDS             137..457
                     /locus_tag="PPY92_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDJ2626707.1"
                     /translation="MKFIIAILLGLIVSITIAFTIIHHPILDRFNPFLKTEYSYAKVP
                     KGTQQYVNITAYSERGEKLDYKLTFNGFSPSRTYVEIKHKGQYVISITYVEKEDIPKE
                     VRQE"
     gene            454..1095
                     /locus_tag="PPY92_002784"
     CDS             454..1095
                     /locus_tag="PPY92_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDJ2626708.1"
                     /translation="MIELKDLTIQKGNIHILKKLNLKFQCGKSYALIGKSGCGKSTLL
                     NTIAGLEKTGKQYVYFNGQLEQFKSNFYRDKLGYLFQNYGLIDNLTVNENLDIGLAYK
                     KISKKEKEQLKIRYIEQFGLSNSLKRKVHTLSGGEQQRVALIRMMLKDPIVMLADEPT
                     GALDPKTGQMIIQSLFDLVDENKVLILATHDMAIANQCDEIIDLEQYSKVASM"
BASE COUNT          475 a          145 c          209 g          383 t
ORIGIN      
        1 tgacgaatgt aattttacat tctcagtata taatgatgat atttgcaacg attcttgtcg
       61 ttcaaatatt attgcagatt tgcagcctat actatcatgg ccgacgtttt aatgaagtta
      121 tcaaggagtt ttaactatga aatttatcat tgcaatatta ttaggattaa tcgtatccat
      181 tactattgct tttacaatca tacatcatcc tatacttgat cgttttaatc ctttcttaaa
      241 aacggagtat agttatgcca aagtgccaaa aggtacgcaa caatatgtta atattacggc
      301 ttatagtgaa agaggggaaa agcttgatta taaattaaca tttaatggat tttcacctag
      361 tagaacgtat gtcgaaataa agcataaagg gcaatatgtc atatcgatca catatgttga
      421 aaaagaggat ataccaaaag aggtaagaca agaatgatag aattaaaaga tttaaccata
      481 caaaaaggta atatacatat tttgaagaaa cttaatttga aatttcaatg tggaaaatca
      541 tatgcactta ttggtaaaag tggatgcggt aaatctacat tattaaatac tattgctgga
      601 cttgaaaaaa cggggaaaca atatgtctat tttaatggtc aattagaaca atttaaatct
      661 aatttttaca gagataaatt aggatattta tttcaaaatt atggattaat cgataatttg
      721 acagtaaatg aaaatttaga tattggatta gcatataaaa aaataagtaa gaaagaaaaa
      781 gaacaattaa agatacgtta tatagaacag tttggtctgt caaacagttt aaaaagaaaa
      841 gttcatacgc taagtggagg tgaacaacaa cgtgtcgctt taattagaat gatgttaaaa
      901 gatccgattg ttatgttagc tgatgaacca acgggtgcgt tagatcctaa aacaggacag
      961 atgattattc aatcattatt tgatttggtc gatgaaaata aagtgctgat tttagcaaca
     1021 catgatatgg ctattgcaaa tcaatgtgac gaaataatag atttagaaca gtatagtaaa
     1081 gtagcatcta tgtgatatcc aaaagataaa attaaaagcc aatcaatggg aaatgttgac
     1141 ttcaacactc cgtttgattg gcttttataa tgtgttacta agttatgaaa gtaacaacgg
     1201 taaactgaaa aa
//
LOCUS       DANBWH010000358         1202 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.369, whole genome shotgun sequence.
ACCESSION   DANBWH010000358 DANBWH010000000
VERSION     DANBWH010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.369"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            52..999
                     /locus_tag="PPY92_002785"
     CDS             52..999
                     /locus_tag="PPY92_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDJ2626709.1"
                     /translation="MKRSSKSKMSLPKKIFLWVFGILVILAIVAVVYVAAKIFITGNK
                     IHNPLDRNHSELRDKKVSLNDGDPFTIALFGVDSDADRKKKGGGERSDSIMILSINPK
                     TKKTEIVSIPRDTRAEIVGRGTTEKIAHAYAYGGPNMAVKSLEKLMNVPIDHYATIDM
                     DGLHNMIDSIGGVDVVSNDTFTVDGVRFTKGQQTHVNGDQALKFIRSRKEEGAGGDFG
                     RQQRQQIVLEAMANKIASPSSITHFNSLMNEIQNNVKTDLTLGDLNTIRSNYKDANDT
                     INKHQLSGQGGIQSDGLYYFIPSEQSKAESTKLLKDNLE"
BASE COUNT          444 a          152 c          247 g          359 t
ORIGIN      
        1 aaatagagaa aacagacaaa gtatgtaagg aatagtaaag gagtggtggg attgaaacgt
       61 tcgtctaaaa gtaaaatgag cctaccgaaa aaaatatttt tatgggtatt cggtatttta
      121 gtaatcttag cgattgtagc tgttgtgtat gtagctgcta aaatttttat tactggtaat
      181 aagatacaca atccgttaga tcgcaatcat tcagaattaa gagataaaaa agtgagttta
      241 aacgatggtg atccattcac aatcgcttta tttggtgtcg attcagatgc tgatcgtaag
      301 aaaaagggtg gcggagagcg tagtgatagt attatgattt tatctatcaa ccctaaaacg
      361 aagaaaactg aaatagtaag tataccgcgt gatacaagag cggaaattgt tggccgtggt
      421 actactgaaa aaattgcaca tgcctatgca tatggtggac caaatatggc tgttaaatca
      481 cttgaaaaat taatgaatgt gccaattgat cattatgcga caatcgatat ggatggtttg
      541 cataatatga tagatagcat cggcggtgtt gatgtagtaa gtaatgatac attcactgtt
      601 gatggtgttc gttttacaaa aggacagcag acacatgtaa atggtgatca agcattgaag
      661 tttattagaa gtcgtaaaga agaaggtgct ggtggtgact ttggtcgaca acaacgtcag
      721 caaattgtgc ttgaagcgat ggccaataag attgcaagcc catcatcgat tacacatttt
      781 aatagtctaa tgaatgaaat tcaaaataat gttaaaacag atttaacatt aggtgatttg
      841 aatacgatta gaagcaatta taaagatgct aatgacacga ttaataaaca tcaattgagt
      901 ggtcaaggcg gcattcaaag tgacggattg tactacttca ttcctagtga gcagtcaaaa
      961 gctgaaagta ctaagttatt aaaagataat ctagagtagt aaactaaggg aaaataagtt
     1021 atcaaaattg tacacaaatt aattagatag aatgatctgt aaatagaata acgaatagaa
     1081 ctaatagccc gtaaatagaa ggtgataaca ctttgtattt atgggctatt ttatttaatg
     1141 cctgatttga tttttataaa ttaatccatt acaaatttaa tttaaagata aatattacct
     1201 tc
//
LOCUS       DANBWH010000359         1182 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.370, whole genome shotgun sequence.
ACCESSION   DANBWH010000359 DANBWH010000000
VERSION     DANBWH010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.370"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(287..1114)
                     /locus_tag="PPY92_002786"
     CDS             complement(287..1114)
                     /locus_tag="PPY92_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDJ2626710.1"
                     /translation="MWKWEAENDAKGVVVIAHNILEHTGRYAYVITMLRRNGYHVIMG
                     DLPGQGQTSRANKGQIENFQTYHESLLDWLKIANEYKIPTYVLGVGLGGLILLNLLEK
                     VELPIEGMMLISPMLELQKNGKNRKDKLVSNIGKISKDTRFNVGVEPKDLTRNLEIVE
                     ETVNDGLMLKKATYHWYNTINETMKDTMAHIHDIQPMPTLLMYGTKDLIVDTRAIDEF
                     KEKYQTPELYFKAWQGFYHEVHNEPERDEVMRYILTFLNNSVNTMGFIVEDDEIVEI"
BASE COUNT          395 a          219 c          130 g          438 t
ORIGIN      
        1 attaaattgc aacaattcta catatatttc ttcaaaaaag atacgcattg aatttttatt
       61 acatataact ctcatgaaaa ttgattaaac taaattaatt agttagcctt aaatttaaca
      121 aattaaatca aaccgaatga ggctaaattg gttatttttc aagatatgat ttgtataaat
      181 caaaacattt cttacaaatc aaatgtgtat aaaatgtgat atacattctc aaccttaaaa
      241 aagtaaaaaa aaagatgagc atctactgtc tcatcttttt agaatattaa atttctacaa
      301 tttcgtcatc ttcaacaata aagcccattg tattgacgct gttatttaag aaagtcagaa
      361 tataacgcat tacttcatca cgttctggct cattgtgaac ctcgtggtaa aaaccttgcc
      421 aagctttaaa atataattca ggtgtttgat atttttcttt aaactcatca attgccctag
      481 tatcaacaat taaatccttc gttccataca ttaatagcgt tggcattggt tgaatgtcat
      541 gaatatgagc catcgtatct ttcatcgtct cattaattgt attataccaa tgatacgttg
      601 ctttttttaa cattaaacca tcgttaactg tttcttcaac aatttctaaa ttacgtgtta
      661 aatcttttgg ttctacacca acattaaaac gtgtgtcttt tgaaatttta cctatatttg
      721 aaacaagttt atctttacga ttttttccat tcttttgaag ttctagcata ggagaaatta
      781 acatcatccc ctcgattggc aattctactt tttcaagtaa atttaataaa atcaaaccgc
      841 caagtcctac ccctaataca taagtaggaa ttttatattc attagctatc tttaaccaat
      901 ctagcaaact ttcgtgatac gtttgaaagt tttcaatttg tcctttatta gctcttgaag
      961 tttgaccttg accaggcaaa tctcccataa tcacatgata gccatttctt cttaacatcg
     1021 taataacata tgcatatctt cccgtatgtt ctaatatatt atgagcaata acaacgacgc
     1081 ctttcgcatc attttcagct tcccacttcc acattattat actgcccctt tttcattaat
     1141 cttcaataac ataattatag caaattcact atgtagattt ct
//
LOCUS       DANBWH010000360         1158 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.371, whole genome shotgun sequence.
ACCESSION   DANBWH010000360 DANBWH010000000
VERSION     DANBWH010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.371"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            106..1158
                     /gene="isdA"
                     /locus_tag="PPY92_002787"
     CDS             106..1158
                     /gene="isdA"
                     /locus_tag="PPY92_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored heme-scavenging protein IsdA"
                     /protein_id="HDJ2626711.1"
                     /translation="MTKHYLNSKYQSEQRSSAMKKITMGTASIILGSLVYIGADSQQV
                     NAATEATNATNNQSTQVSQATSQPINFQVQKDGSSEKSHMDDYMQHPGKVIKQNNKYY
                     FQTVLNNASFWKEYKFYNANNQELATTVVNDNKKADTRTINVAVEPGYKSLTTKVHIV
                     VPQINYNHRYTTHLEFEKAIPTLADAAKPNNVKPVQPKPAQPKTPTEQTKPVQPKVEK
                     VKPTVTTTSKVEDNHSTKVVSTDTTKDQTKTQTAHTVKTAQTAQEQNKVQTPVKDVAT
                     AKSESNNQAVSDNKSQQTNKVTKHNETPKQASKAKELPKTGLTSVDNFISTVAFATLA
                     LLGSLSLLLFKRKESK"
BASE COUNT          493 a          228 c          156 g          281 t
ORIGIN      
        1 tatacggaaa tagatgtgct agtataattg ataaccatta tcaattgcaa tggttaatca
       61 tctcatataa caacacataa tttgtatcct taggaggaaa acaacatgac aaaacattat
      121 ttaaacagta agtatcaatc agaacaacgt tcatcagcta tgaaaaagat tacaatgggt
      181 acagcatcta tcattttagg ttcccttgta tacataggcg cagacagcca acaagtcaat
      241 gcggcaacag aagctacgaa cgcaactaat aatcaaagca cacaagtttc tcaagcaaca
      301 tcacaaccaa ttaatttcca agtgcaaaaa gatggctctt cagagaagtc acacatggat
      361 gactatatgc aacaccctgg taaagtaatt aaacaaaata ataaatatta tttccaaacc
      421 gtgttaaaca atgcatcatt ctggaaagaa tacaaatttt acaatgcaaa caatcaagaa
      481 ttagcaacaa ctgttgttaa cgataataaa aaagcggata ctagaacaat caatgttgca
      541 gttgaacctg gatataagag cttaactact aaagtacata ttgtcgtgcc acaaattaat
      601 tacaatcata gatatactac gcatttggaa tttgaaaaag caattcctac attagctgac
      661 gcagcaaaac caaacaatgt taaaccggtt caaccaaaac cagctcaacc taaaacacct
      721 actgagcaaa ctaaaccagt tcaacctaaa gttgaaaaag ttaaacctac tgtaactaca
      781 acaagcaaag ttgaagacaa tcactctact aaagttgtaa gtactgacac aacaaaagat
      841 caaactaaaa cacaaactgc tcatacagtt aaaacagcac aaactgctca agaacaaaat
      901 aaagttcaaa cacctgttaa agatgttgca acagcgaaat ctgaaagcaa caatcaagct
      961 gtaagtgata ataaatcaca acaaactaac aaagttacaa aacataacga aacgcctaaa
     1021 caagcatcta aagctaaaga attaccaaaa actggtttaa cttcagttga taactttatt
     1081 agcacagttg ccttcgcaac acttgccctt ttaggttcat tatctttatt acttttcaaa
     1141 agaaaagaat ctaaataa
//
LOCUS       DANBWH010000361         1146 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.372, whole genome shotgun sequence.
ACCESSION   DANBWH010000361 DANBWH010000000
VERSION     DANBWH010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.372"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(68..256)
                     /locus_tag="PPY92_002788"
     CDS             complement(68..256)
                     /locus_tag="PPY92_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDJ2626712.1"
                     /translation="MRNQIQKLLDSDLSSLHISKQTGVPQSTIHRMRKKERSLDNMSL
                     KNAELLYKFANSIFSNEN"
     gene            505..>1146
                     /locus_tag="PPY92_002789"
     CDS             505..>1146
                     /locus_tag="PPY92_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDJ2626713.1"
                     /translation="MRLQKAPLVTSGLVLGLLGLGNLLKDLSLTLNAVCGIFAFLIWI
                     HLLCTMIKYFNNVKEQLNSPLVSSVFTTFFMSGFLGTTYLNTFFSNITFINSLITPIW
                     ILCLVGIMTHMIIFSIKYLKDFSLENVYPSWTVLFIGIAIAGLTAPVSGCFFIGQLTV
                     IYGFVATCIVLPIVFKRLKAFPLQTSIKPNTSTICAPFSLVAAAYVIAFPKANA"
BASE COUNT          344 a          192 c          159 g          451 t
ORIGIN      
        1 atttaaaaaa ataaccacgt ccatcgagac gtggtttttt aattatttaa ctcactaatt
       61 attcttctta attttcattg ctaaatatac tattggcaaa tttataaagt agttcagcgt
      121 ttttcaatga catattgtct aatgatcttt ctttttttct cattctgtgt attgtgcttt
      181 gtggaactcc tgtttgtttc gatatatgta aactgctcaa atcactgtct aatagttttt
      241 gaatttgatt tctcattttg tacaccccct tgtaaatctt caatcatcat ttaaattaaa
      301 taattacttt tccacatatt ccaatattta ggttgcaaag catacctcaa atatcattaa
      361 ttttgagatt taaatgtcaa ttatgtgtct atcaatccaa tatacatact ctaataacgt
      421 aatagtacac actcttctta ttaattggcc atagctatca tgatataatt agtgaagaaa
      481 atcacataag aaaggttgta aatcatgaga cttcaaaaag cacctctagt aacgtcagga
      541 ctagtcttag gattattagg cctgggtaat ctattaaaag acttatctct tactttaaac
      601 gctgtttgcg gaatctttgc tttcttgatt tggattcacc ttttatgtac tatgatcaaa
      661 tattttaata atgtgaaaga acaattaaac agtcctctag tttcatcagt gttcacaaca
      721 tttttcatgt ctggcttttt aggtactact tatttaaata cattttttag taacataact
      781 tttatcaata gcttaataac gcctatttgg attttatgcc ttgtgggaat tatgacgcat
      841 atgattattt tttcaataaa atatttaaaa gatttttcac ttgaaaatgt ttatccttcg
      901 tggactgtac tttttattgg tattgctatc gcaggattga cggcacccgt tagcggatgt
      961 tttttcatag gtcaattaac agtaatatat ggctttgtag ctacttgtat tgtcttacct
     1021 attgttttca agcgattaaa agcatttcca ttgcagacgt caatcaaacc gaacacatcg
     1081 acaatttgtg caccattttc tttagtcgct gcagcatatg ttatagcctt tcctaaggcg
     1141 aatgct
//
LOCUS       DANBWH010000362         1135 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.373, whole genome shotgun sequence.
ACCESSION   DANBWH010000362 DANBWH010000000
VERSION     DANBWH010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.373"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(98..949)
                     /locus_tag="PPY92_002790"
     CDS             complement(98..949)
                     /locus_tag="PPY92_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HDJ2626714.1"
                     /translation="MQQFKWINILKGFAMGTSDLVPGVSGGTIALLLGIYNQFIASIS
                     GIFSRRFWPSFTFLIPIIIGMLLAMGSLSNLFNYLLSQHHIPTMFFFGGLIIGIVPYL
                     LKISNYKTSFTTKHYMMVIAGIAILIVITLMNNGDKHAGETLTLSTGLIIKYFIAGMC
                     ASSAMLLPGISGSFMLLVFGVYGTVMLAISEVVKLNFTGLPILLAVGFGVLAGFIISS
                     KIIQYFLTHHKLMTFALIIGFVVGSLFAVFPGLPTNIVMWFVSLVVFIIGFIVSLTLG
                     RITAENE"
BASE COUNT          447 a          187 c          171 g          330 t
ORIGIN      
        1 aactcgctaa tgatatccaa taatatgtat acaaaacgag acatatattg catatgatta
       61 acgagatact gaatatattt tatcacccct aaaatgatta ttcattttca gcggtaattc
      121 gacctaaagt caaacttaca ataaaaccga tgataaatac tactaatgaa acgaaccaca
      181 tcacgatatt agttggtaaa cctggaaata ctgcaaagag ggagccaaca acaaaaccaa
      241 tgattaatgc aaaagtcatt agtttatgat gtgttaggaa atactggata attttgcttg
      301 aaataatgaa tccagcaagc accccaaatc cgactgcaag tagtatagga agaccggtaa
      361 agttaagttt aacaacttca gatattgcta gcatgaccgt accatagacg ccaaatacta
      421 ataacataaa tgatcctgaa atacctggga gtaacatagc gctagatgca cacatacctg
      481 caataaagta tttaataata agaccagttg atagagtaag cgtttctcca gcatgtttat
      541 caccattatt cattaatgta ataacaatta agatagcgat accagctata accatcatgt
      601 aatgtttagt tgtaaatgac gttttatagt tagaaatttt caataaatat ggaacgatac
      661 caatgattaa tccaccaaag aaaaacatag ttggaatatg gtgttggctt aataaataat
      721 taaaaagatt acttagtgat cccattgcca gtaacattcc aattataatg gggattaaaa
      781 atgtaaaact tggccaaaaa cgtcgtgaga atatgccgct aattgaagcg ataaattgat
      841 tgtaaatacc taacaataat gcgatagtcc caccgctaac accaggtacc aagtcactcg
      901 ttcccatagc aaaacctttt agaatattaa tccatttaaa ttgttgcatg aataactcct
      961 ttcaaacgat tggaataaaa tcataaatag catcatacca tattacaaat gtcctagtga
     1021 aatgataaca tattttaaat tcataaaatc cattgagaaa ttatgtgcac ttattatcat
     1081 ttatattttt aaagagagcg gattagagtt gtactttaga gttattaaaa ataat
//
LOCUS       DANBWH010000363         1119 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.374, whole genome shotgun sequence.
ACCESSION   DANBWH010000363 DANBWH010000000
VERSION     DANBWH010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.374"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
BASE COUNT          430 a          184 c          141 g          364 t
ORIGIN      
        1 aagtttagtt aagggttctt ctcaacgcac aataaatttt ctcggcataa atgcgtgtct
       61 atgcgtagac atggagacac ttcagaatta tggagtgatt acatgagttt aactattttt
      121 ctttattcag ttttaggaag tttattagca aattatatct acgaaaaatc ttcaaaataa
      181 ataacttttt cggttctact ttgtacactt tatctacctg gtagacctaa ttgttattgc
      241 tttacaggtc taaaattcgt cgttataatc tagttaaatc ccgtaagggc gcacttatac
      301 gtcggataaa tcgacatgtg caaaaacgta atcgaccttc gtcgattttt ttatttcaaa
      361 attaaagaaa cggcttgaga tattccgagc agttataaaa attaatttgc gttgaatatt
      421 gctactacat tcttgaaaaa gtgatgtaga tttatagccc ataacttttt tgaattacat
      481 cgacgatacg gcgtcgaaag aaaaataaat agaccgtatg aaagaaaaag ctacctcact
      541 ttatctgggg tagcttttat tttttatgcc cttattttga attttaagag ggcattttaa
      601 agatttaggg gtgattcata tagtttttat atctaaaact tatatatgct tttaaaacgc
      661 aaatatgagc caaataaata tatgctattt tcaaaaacaa aaatttgagc aaaaacaata
      721 actattttta gacactgccc atttacatgc aaattaaaaa ttagcataaa attctttaaa
      781 acttccccac gtttttctaa attcctgtag ggcgttttta tcaaaaatca agacaagaag
      841 aaactcgttc acactcgttt caaaaaaaca agaaatctaa tctatacacc taccgttttc
      901 caaccccctc aaagcataca aattaaaaac aaaattattc ataatcttac tttcttttaa
      961 caaaaacata aaaaccataa atagacaaat taactatcaa aggcaataaa cagtataaaa
     1021 aaaccacgaa taaatcttca tcaacatcta aaataaaaaa gaataaaaca attaacaaaa
     1081 tactaattaa caaacctaaa agtggcaaac tttttatat
//
LOCUS       DANBWH010000364         1100 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.375, whole genome shotgun sequence.
ACCESSION   DANBWH010000364 DANBWH010000000
VERSION     DANBWH010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.375"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..914)
                     /locus_tag="PPY92_002791"
     CDS             complement(<1..914)
                     /locus_tag="PPY92_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HDJ2626715.1"
                     /translation="MEEFKQHYKGLIDESLTCQDKVELIKKCEKYTDEVIRKDVLPED
                     IVDIHKNYILTLNLTREDVFKTLDVLQEIVKGFGYSYRDYQRLVDKLQVHDKEIDLAS
                     SLQQTMLKTDIPQFDSIQIGVISVAAQKVSGDYFNLIDHNDGTMSFAVADVIGKGIPA
                     ALAMSMIKFGMDSYGHSQLPSDGLKRLNRVVEKNINQNMFVTMFYGLYEEMNHLLYCS
                     SAGHEPGYIYRAEKEEFEEISVRGRVLGISSQTRYQQQEIPIYLDDLIIILTDGVTEA
                     RNSEGTFIDKQKLLEYIKKHKHMHPQDIV"
BASE COUNT          344 a          212 c          143 g          401 t
ORIGIN      
        1 acaatatctt gtgggtgcat atgtttatgt tttttaatat attctaaaag tttttgttta
       61 tctataaagg taccttcact atttctagct tcagtcacac catccgttaa aatgataatt
      121 aaatcatcaa ggtatatagg aatttcttgt tgttgatatc gtgtttgtga actgattcct
      181 aacactctac ctctaactga aatttcttca aattcttctt tttcagcgcg ataaatatat
      241 ccaggctcat gaccagctga actacaatac aataaatggt tcatttcttc atataaacca
      301 taaaacattg tgacgaacat attttgatta atattctttt caacaacacg atttaaacgt
      361 tttaagccat cactcggtaa ttgtgagtgt ccataagaat ccatgccaaa ctttatcata
      421 ctcattgcta aagcagctgg tatacctttc ccaatgacat ctgcaacagc aaagctcatt
      481 gtgccatcgt tatggtcaat taaattaaaa taatctccac ttactttttg tgctgccact
      541 gaaataacgc caatttgaat actatcaaat tgtggaatat ctgttttaag cattgtttgt
      601 tgtaagctag aagctaagtc tatctcttta tcgtgaactt gaagtttatc taccaatctt
      661 tgataatctc gataactata accaaagcct ttaacgattt cttgtaagac atctaatgtc
      721 ttgaacacat cttcacgcgt taagtttaac gtcaatatat agtttttgtg aatatcgaca
      781 atgtcttcag gcaagacgtc cttacgaatc acttcgtcag tgtatttctc acactttttt
      841 atcaattcta ctttatcttg gcacgttaaa ctttcatcaa ttaaaccctt ataatgttgc
      901 ttaaattctt ccacgattag ttgcctcctc acttccctta caaagcaaat gcttaagcaa
      961 ggataattat gattcactta ctaacattta tttaattgca cttatcaatt ttgtcgtttg
     1021 caattttgct gtagatgata cttttaagct aaaaaaactt taggatggaa tacctaaagc
     1081 ctaaaaaact taagacatcc
//
LOCUS       DANBWH010000365         1098 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.376, whole genome shotgun sequence.
ACCESSION   DANBWH010000365 DANBWH010000000
VERSION     DANBWH010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.376"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(115..>1098)
                     /locus_tag="PPY92_002792"
     CDS             complement(115..>1098)
                     /locus_tag="PPY92_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase C"
                     /protein_id="HDJ2626716.1"
                     /translation="KKCIKTLFLSIILVVMGGWYHSAHASDSLSKSPENWMSKLDESK
                     HLTEINMPGSHDSGSFTLTDPVKSVWAKTQGKDYLTQMKSGVRFFDIRGRASADNMIS
                     VHHGMVYLHHELGKFLDDAKYYLSAYPNETIVMSMKKDYDSDSKVTKTFEEIFREYYY
                     NNPQYQNLFYTGSNANPTLKETKGKIVLFNRMGGTYIKSGYGADTSGIQWADNATFET
                     KINNGSLNLKVQDEYKDYYDKKVEAVKNLLAKAKTDSNKDNVYVNFLSVASGGSAFNS
                     TYNYASHINPEIAKTIKENGKARTGWLIVDYAGYPWPGYDDIVSEIIDSNK"
BASE COUNT          336 a          222 c          134 g          406 t
ORIGIN      
        1 ctgttcaatt tataatattg ttgaattatt acttattttc aaaataaaaa cgagtagtga
       61 aaataattag aatctaacta ttttcatact cgtcttaata tcattattga atccttattt
      121 attactatct ataatttcac ttacgatatc atcatatcca ggccacggat atcctgcata
      181 gtcaacaatc agccaaccag ttctagcttt cccgttttct ttaatcgttt ttgcaatttc
      241 aggatttata tgtgatgcat agttataagt actattaaat gcgctacctc cagacgctac
      301 actcaagaaa ttcacatata cattgtcttt gttactatcc gttttagctt tagccaataa
      361 atttttaaca gcttcaactt ttttatcata gtaatcttta tactcatctt gtacttttaa
      421 atttaagcta ccattattaa ttttcgtttc aaatgtcgca ttgtctgccc attgaatacc
      481 tgacgtgtca gcaccataac cactctttat gtatgtaccc cccattctat tgaataggac
      541 aattttacct ttcgtttctt ttaaagtagg attcgcatta cttcctgtgt aaaaaagatt
      601 ctgatattgc gggttattat aataatattc tctaaaaatc tcttcaaatg tcttcgtaac
      661 tttagaatcg ctatcgtagt cctttttcat agacatcaca attgtttcgt ttggataagc
      721 actcaagtaa tatttagcat catctaaaaa ttttcctagt tcatgatgca aataaaccat
      781 gccgtgatga actgaaatca tattatcagc acttgctcta cctctaatat caaaaaacct
      841 gactcccgac ttcatttgag taaggtaatc tttgccttga gtctttgccc aaaccgattt
      901 taccggatca gttaaagtga atgagccact atcatgtgag cctggcatat taatctcagt
      961 taaatgtttg ctttcatcaa gtttactcat ccaattttct ggacttttac tcaacgaatc
     1021 tgacgcatgt gctgaatgat accaaccacc catcactact aaaatgatac ttaaaaacaa
     1081 agtcttaata cacttttt
//
LOCUS       DANBWH010000366         1091 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.377, whole genome shotgun sequence.
ACCESSION   DANBWH010000366 DANBWH010000000
VERSION     DANBWH010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.377"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..605
                     /gene="ribF"
                     /locus_tag="PPY92_002793"
     CDS             <1..605
                     /gene="ribF"
                     /locus_tag="PPY92_002793"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HDJ2626717.1"
                     /translation="IAGFDFTFGKFGKGNMTVLQEYDAFNTTIVSKQEIENEKISTTS
                     IRQDLINGELQKANDALGYIYSIKGTVVQGEKRGRTIGFPTANIQPSDDYLLPRKGVY
                     AVSIEIGTENKLYRGVANIGVKPTFHDPNKAEVVIEVNIFDFEDNIYGERVTVNWHHF
                     LRPEIKFDGIDPLVKQMNDDKSRAKYLLAVDFGDEVAYNI"
     gene            720..989
                     /gene="rpsO"
                     /locus_tag="PPY92_002794"
     CDS             720..989
                     /gene="rpsO"
                     /locus_tag="PPY92_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDJ2626718.1"
                     /translation="MAISQERKNEIIKEYRVHETDTGSPEVQIAVLTAEINAVNEHLR
                     THKKDHHSRRGLLKMVGRRRHLLNYLRSKDIQRYRELIKSLGIRR"
BASE COUNT          380 a          162 c          198 g          351 t
ORIGIN      
        1 tcattgctgg ttttgatttt acttttggta aatttggaaa aggtaatatg actgttcttc
       61 aagaatatga tgcgtttaat acgacaattg tgagtaaaca agaaattgaa aatgaaaaaa
      121 tttctacaac ttctattcgt caagatttaa tcaatggtga gttgcaaaaa gcgaatgatg
      181 ctttaggcta tatttattct attaaaggca ctgtagtgca aggtgaaaaa aggggaagaa
      241 ctattggctt cccaacagct aacattcaac ctagtgatga ttatttgtta cctcgtaaag
      301 gtgtttatgc tgttagtatt gaaatcggca ctgaaaataa attatatcga ggggtagcta
      361 acataggtgt aaagccaaca tttcatgatc ctaacaaagc agaagttgtc atcgaagtga
      421 atatctttga ctttgaggat aatatttatg gtgaacgagt gactgtgaat tggcatcatt
      481 tcttacgtcc tgagattaaa tttgatggta tcgacccatt agttaaacaa atgaacgatg
      541 ataaatcgcg tgctaaatat ttattagcag ttgattttgg tgatgaagta gcttataata
      601 tctagagttg cgtatagtta tataaacaat ctataccaca cctttttctt agtaggtcga
      661 atctccaatg cctaactcgg attaaggagt attcaaacat tttaaggagg aaattgatta
      721 tggcaatttc acaagaacgt aaaaacgaaa tcattaaaga ataccgtgta cacgaaactg
      781 atactggttc accagaagta caaatcgctg tacttactgc agaaatcaac gcagtaaacg
      841 aacacttacg tacacacaaa aaagaccacc attcacgtcg tggattatta aaaatggtag
      901 gtcgtcgtag acatttatta aactacttac gtagtaaaga tattcaacgt taccgtgaat
      961 taattaaatc acttggtatc cgtcgttaat cttaatataa cgtctttgag gttggggcat
     1021 atttatgttc caaccttaat ttatattaaa aaagcttttt acaaatatta acatttatta
     1081 tatgttaagc t
//
LOCUS       DANBWH010000367         1082 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.378, whole genome shotgun sequence.
ACCESSION   DANBWH010000367 DANBWH010000000
VERSION     DANBWH010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.378"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            17..910
                     /locus_tag="PPY92_002795"
     CDS             17..910
                     /locus_tag="PPY92_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDJ2626719.1"
                     /translation="MKKTILLTMTTLTLFSMSPNSAQAYTNDSKTLEEAKRAHPNAQF
                     KVNKDTGAYTYTYDKNNTPNNNHQNQSRTNDNHQHANQRDFNNNQYHSSLSGQYTHIN
                     DAIDSHTPPQTSPSNPLTPAIPNVEDNDDELNNAFSKDNKGLITGIDLDELYDELQIA
                     EFNDKAKTADGKPLALGNGKIIDQPLITSKNNLYTAGQCTWYVFDKRAKDGHTISTFW
                     GDAKNWAGQASSNGFKVDRHPTRGSILQTVNGPFGHVAYVEKVNIDGSILISEMNWIG
                     EYIVSSRTISASEVSSYNYIH"
BASE COUNT          403 a          212 c          177 g          290 t
ORIGIN      
        1 ggattaaatg gttattatga agaaaacaat tttactgacg atgacaactc ttactttatt
       61 tagtatgtcg cctaactcgg ctcaagcata tacgaatgat agcaaaacat tagaagaagc
      121 aaagagagca cacccaaacg cacagttcaa agtgaataaa gacaccggcg cgtatactta
      181 tacatatgac aaaaacaaca cgccaaacaa caatcatcaa aaccagtcac gtacaaacga
      241 caatcatcaa cacgcaaatc aacgtgattt taacaacaat cagtaccatt cttcattaag
      301 tggtcagtat acgcacatta atgacgcaat tgattcacac acaccgcctc aaacgtcacc
      361 aagcaatcct ttgacaccag caataccgaa tgtcgaagac aatgacgatg aattaaataa
      421 cgctttttca aaagataaca aagggcttat tacaggcatc gatttagacg aattgtatga
      481 cgaattacaa atcgccgaat ttaatgacaa agcaaagact gctgacggta aacctttagc
      541 attaggtaac ggtaaaatca ttgatcagcc tcttatcaca agtaagaaca acttatatac
      601 tgctggacaa tgtacatggt atgtctttga taaacgtgcc aaagatggac acacgattag
      661 tacattttgg ggagatgcta aaaactgggc aggccaagct tcaagcaatg gcttcaaagt
      721 agatagacac ccaacacgag gatcaatttt acaaacagta aatggtccat ttggtcatgt
      781 agcctacgtt gaaaaagtta atattgatgg aagtattcta atttcagaaa tgaactggat
      841 tggtgaatat atcgtttcat caagaaccat ctctgcttca gaagtttcat catataatta
      901 catccattaa attaatcatg acatcaataa aaagcgacca gttcgcagtt tacaattcgt
      961 aacactgcaa aattggtcgc tttattttgt atgttattcg attattaaat atgatgttta
     1021 ccttcaaaat tgcaataact tgtcacctta aagttaaccg acatttttca agaatcatta
     1081 tg
//
LOCUS       DANBWH010000368         1068 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.379, whole genome shotgun sequence.
ACCESSION   DANBWH010000368 DANBWH010000000
VERSION     DANBWH010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.379"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..291
                     /gene="lpl8"
                     /locus_tag="PPY92_002796"
     CDS             <1..291
                     /gene="lpl8"
                     /locus_tag="PPY92_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl8"
                     /protein_id="HDJ2626720.1"
                     /translation="LKNYSNGDVSINENVPSYDAKFKMSNKDENVKQLRSRYNIPTDK
                     APVLKMHIDGDLKGSSVGYKKLEIDFSKGEKSDLSVIDSLNFQPAKVDEDDE"
     gene            310..1068
                     /locus_tag="PPY92_002797"
                     /pseudo
     CDS             310..1068
                     /locus_tag="PPY92_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498990.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
BASE COUNT          443 a          114 c          216 g          295 t
ORIGIN      
        1 ttgaaaaatt acagcaatgg tgatgtctca attaatgaga atgttccaag ttatgacgca
       61 aaatttaaaa tgagcaataa agatgaaaat gttaagcaat taagaagtcg ttataatatt
      121 cctactgata aagcaccggt attaaaaatg catattgatg gtgatttgaa aggaagttct
      181 gtgggttata aaaagttgga aattgacttt tcaaaaggtg aaaaaagcga tttgtcagta
      241 atagattctt taaatttcca gccggcgaag gtggatgaag atgatgaatg atgaggatgg
      301 tgtgtaacaa tgaagtctat aaaaaggatt ggattgtgca taagtctgtt gattttaatc
      361 atttttgcaa catcttgtgg tagtaataag attactggag actcaaaaga agaacaaatc
      421 aaaaagagtt ttgcgaaatc tttagacaag tatcctactg aaaatctaga agagttttat
      481 gataaagaag gatatagaga tggagaattt gaaaaagatg ataaagggac ttggctaatt
      541 agatctgaaa tgaaaatcca attaaaagga gaaaatctgg aatctagggg agcagttata
      601 gaaattaaca gaaatactag aacggctaaa gggaattata ttgttagaga agttgttgaa
      661 gatagcgatg gaatgacaca caatcataca aaaaggtatc ctgtgaaaat ggaaaataat
      721 aaaatgattc cattaaaatc aattgatgat gaaaaagtaa aaaaagaaat tgaagaattt
      781 aaattctttg tacaatacgg gaatttcaaa gaattggaaa actataaaga ggacgaagtg
      841 tcatataacc cagaagtacc aatatactct gcacaatagc aattgaaaaa cagtgattat
      901 aatgtagagc aattacgtaa gcgatataat atcccgacgc aaaaagcgcc caaattatta
      961 ttgaaaggct caggtaattt aaaaggttca tcagtcggat ataaaaatat tgaatttacc
     1021 tttgttgaaa ataaggaaga aaatatttac ttcacagata gtgtctac
//
LOCUS       DANBWH010000369         1062 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.380, whole genome shotgun sequence.
ACCESSION   DANBWH010000369 DANBWH010000000
VERSION     DANBWH010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.380"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            61..>1062
                     /locus_tag="PPY92_002798"
     CDS             61..>1062
                     /locus_tag="PPY92_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retron St85 family RNA-directed DNA polymerase"
                     /protein_id="HDJ2626721.1"
                     /translation="MEKIENEIVNKTYLAINSLEELRNMIGIKSDYFYKCLYVNDHFY
                     NVIKIPKRKKDEYRELMIPNMALKNIQRWILDNVLYRRQVHKCATGFVPRKSIVNNAI
                     PHVGQKYILKMDIENFFPSITFKQVRKIFSEMGYKFELATALANLCTVNNQLPQGAPT
                     SPYIANIIFYNIDKRIFSYCQKNNLRYTRYADDITISGSNKVSFSKEIIREIVNQYNF
                     RINESKTIMFKPGDRKKVTGIIVNEKISVPKTLIREVRKQIYFVNKFGLEEHLIRNNY
                     SLDYEQQFIMSIYGKISFIKMIDFKKGVSLQKKFNEVLGNIESSNMYRDNIDFDDIEL
                     "
BASE COUNT          430 a          110 c          180 g          342 t
ORIGIN      
        1 ataattaaaa gaagatggtt tagcaagagt tgctcattta gataaaaaat ggagaattaa
       61 atggagaaaa ttgagaatga aatagttaat aagacatatc ttgcaattaa ttctttggaa
      121 gaattaagaa atatgattgg tattaaatca gactattttt ataaatgtct ttatgtgaat
      181 gaccattttt ataatgttat caaaatacct aaacgaaaaa aagatgaata tagagaattg
      241 atgattccta atatggctct caaaaatatc caaaggtgga ttttagataa tgtattgtac
      301 agaaggcaag ttcataaatg tgcaactggt tttgttccta ggaaatcaat agtgaacaat
      361 gcaatcccac acgttgggca gaaatatata ttgaaaatgg atatagaaaa cttttttccg
      421 agtatcacat ttaagcaggt tagaaaaata ttttctgaga tgggctataa atttgaactt
      481 gcgacagcac tcgcaaattt gtgtacagtg aataatcagt tgcctcaagg tgcaccaacc
      541 agtccatata tagctaatat catcttttac aatatagata aaagaatctt ttcttattgt
      601 caaaaaaata atttgagata tactcgatat gctgatgata ttactatttc tggatctaat
      661 aaagtaagtt ttagtaaaga aattatcagg gaaatagtaa accaatataa ttttagaatt
      721 aatgaaagta agacaattat gtttaaacca ggagacagaa aaaaagttac tggtattata
      781 gttaatgaaa aaatatcggt tcctaaaact ttaataagag aggtcaggaa gcaaatttat
      841 tttgtaaata agtttggatt agaagaacac ttaattcgaa ataattattc attggattat
      901 gagcagcaat ttattatgag tatatatgga aaaatcagtt ttattaaaat gattgatttc
      961 aagaaaggcg ttagtttaca aaagaaattt aatgaagtac taggtaatat agaatcgtct
     1021 aacatgtaca gagataatat tgactttgat gatatagaac ta
//
LOCUS       DANBWH010000370         1053 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.381, whole genome shotgun sequence.
ACCESSION   DANBWH010000370 DANBWH010000000
VERSION     DANBWH010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.381"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(137..757)
                     /locus_tag="PPY92_002799"
     CDS             complement(137..757)
                     /locus_tag="PPY92_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDJ2626722.1"
                     /translation="MEQLPQVGFMKSFVLFWKNYVNFKGRSRRSEYWYMALWHLILMV
                     PAVCVLIINLFLVFISIAADNETLIVMSILMIILTSVYTFVYSLATFIPNLALCVRRF
                     HDISRTMLFPMIMTVFSIIFYIVLQIIDSYYDSDFTLMPMGLNILLVLLYFVYLGLTI
                     VMIVFLCFDSKPANKYGESPKYPSTIKNQPVTSETPKTPEETDNQL"
BASE COUNT          371 a          185 c          134 g          363 t
ORIGIN      
        1 tctgaaattg aaatatttaa atagtatctt ctttgcatgt taagtatcat tagcgctaga
       61 tacgcatttc ccataccttt attgccttat ttgtaaaaaa atatcccagc atcattgtgc
      121 cgagatattt atatcattac aattgattat ctgtttcttc cggtgttttc ggtgtttcag
      181 atgtcactgg ttgattttta attgtagacg ggtattttgg gctttcgcca tatttatttg
      241 ccggcttact gtcaaagcat aggaatacta tcataacaat tgttaggcct aaataaacaa
      301 aataaagaag tactaacaaa atattaagac ccatcggcat taatgtaaaa tcactgtcat
      361 aataactatc gataatctgt aatactatat aaaatataat actgaatact gtcataatca
      421 ttggaaataa cattgttctt gatatatcgt gaaatcttcg aacgcacaac gctaaatttg
      481 gaataaacgt tgccaaacta tagacaaaag tatacacaga tgtaaggata atcatcaata
      541 tactcataac tattaatgtt tcgttatctg ccgctataga aataaagact agaaataggt
      601 ttattattag cacacacaca gctggaacca taagtatcaa atgccatagt gccatatacc
      661 aatattcact acgtcttgat ctccccttaa aatttacata atttttccaa aataatacga
      721 atgatttcat aaaacctact tgaggtaatt gttccattgt aatctccctt tcgttaatca
      781 tatttatatt tttaattatt gttaccgtta taatttacaa gattcattat caagccactt
      841 catttcaatc gtcattcact ttggaaaatg gactttatat ttcatcacta gtaaaatgaa
      901 aacccgctac aagtacacat ctatatggag actcatttga aagtcaacgc ttcgttaact
      961 atactaaaaa tatgtcatac tgcaatgttc acgtttaaaa gagtctcaat ctctgcaaat
     1021 aaaatattcc ataacaaagt atatacttta cat
//
LOCUS       DANBWH010000371         1038 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.382, whole genome shotgun sequence.
ACCESSION   DANBWH010000371 DANBWH010000000
VERSION     DANBWH010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.382"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..129
                     /locus_tag="PPY92_002800"
     CDS             <1..129
                     /locus_tag="PPY92_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein"
                     /protein_id="HDJ2626723.1"
                     /translation="KITGTDNNYTIDLSKRLPSTDANRYVKKPQNAKIEVILEKSN"
     gene            237..953
                     /locus_tag="PPY92_002801"
     CDS             237..953
                     /locus_tag="PPY92_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL12"
                     /protein_id="HDJ2626724.1"
                     /translation="MSKNITKNIILTTTLLLLGTVLPQNQKPVFSFYSEAKAYSIGQD
                     ETNINELIKYYTQPHFSFSNKWLYQYDNGNIYVELKRYSWSAHISLWGAESWGNINQL
                     KDRYVDVFGLKDKDTDQLWWSYRETFTGGVTPAAKPSDKTYNLFVQYKDKLQTIIGAH
                     KIYQGNKPVLTLKEIDFRAREALIKNKILYTENRNKGKLKITGGGNNYTIDLSKRLHS
                     DLANVYVKNPNKITVDVLFD"
BASE COUNT          413 a          152 c          155 g          318 t
ORIGIN      
        1 aaaataacag gtacagacaa taactacaca atagatttaa gtaaaaggtt gccatcaact
       61 gatgcaaata gatatgttaa aaaacctcaa aatgcaaaaa ttgaagttat cctcgaaaaa
      121 tcaaactaac aataataatg gagttaataa aaataatcgc aaatactata ttgacttcgc
      181 tcacatttaa atttcttatt cctcgtatca tgattcctct gaaaggagat gttctaatga
      241 gtaagaacat cacgaaaaat ataattttaa cgacaacatt attactatta ggtactgtat
      301 tacctcaaaa tcaaaaacca gtatttagtt tttactctga agctaaagct tatagcattg
      361 gtcaagatga aactaacatc aatgaattaa ttaaatatta cacacagcct catttttcat
      421 tttcaaataa atggctatat caatatgata atggaaacat ttatgttgaa cttaagagat
      481 attcatggtc agcacatata tctttatggg gcgctgaaag ttggggaaat attaatcagt
      541 taaaagatcg ttacgtagat gtgtttggac taaaagacaa agatactgat cagttatggt
      601 ggtcttatag agagacattt acaggtggcg ttacaccagc cgcaaaacct tctgataaaa
      661 cttataatct ttttgtgcaa tacaaagata aactacaaac gattattggt gcgcataaaa
      721 tataccaagg caataaacca gtattaacat tgaaagaaat cgatttccgt gcacgagaag
      781 cgttaataaa aaataaaata ttatataccg aaaatcgtaa taaaggtaag cttaagatca
      841 ccggtggcgg taataactac actattgatt taagcaaaag attacattca gatctagcaa
      901 atgtttatgt taaaaatcct aataaaataa ctgttgacgt cctctttgat tagtatatga
      961 aggtgactta tacttcatgc actttaattc caaatcagat tatttaaatg ataattttta
     1021 aagtgtatga tgtatata
//
LOCUS       DANBWH010000372         1037 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.383, whole genome shotgun sequence.
ACCESSION   DANBWH010000372 DANBWH010000000
VERSION     DANBWH010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.383"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            58..984
                     /locus_tag="PPY92_002802"
                     /pseudo
     CDS             58..984
                     /locus_tag="PPY92_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428224.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
BASE COUNT          272 a          184 c          185 g          396 t
ORIGIN      
        1 tcttgtattt ctattaaacc ttattatctt tcccatagat tctaaagtct tactttcggt
       61 gcaattttct tagcaagctc tgtgggtagt acattctcaa caatcaaccc attctcagtc
      121 gtcattgctt ctaatgcagc aggaacaact tttactgatg gtctttattg gagaataggc
      181 gcttgtatca tcggtgccat atttgttatt agttatttat tctggtattg taaaaaaatt
      241 aaaaaagatc ctaaatcctc ttattcttat gaagacaaag cagcatttga aaaacagtgg
      301 tctgtgctcc atgatgacgg ttcttctgag tttacattac gtaaaaagat tattcttacg
      361 cttttcgtcc taccattccc tattatggtt tggggtgtta tgacacaagg atggtggttc
      421 ccagtcatgg catctgcatt cttaatcttt accattgtca tcatgtttat tgctggaaca
      481 ggacaatatg gtttaggcga aaaaggcact gtagatgcat tcgttaatgg cgcttcaagt
      541 ttagtaggtg tatctttaat cattggttta gctcgtggaa tcaacttagt attgaataaa
      601 ggaatgattt ctgacacaat cttgcacttt tcatcatcta tcgtgcaaca tatgagtggg
      661 cctttattta tcattgttct gctctttatc tttttctgtt taggatttat cgtaccatca
      721 tcatcaggat tagcagtatt atctatgcct atctttgcgc cattagctga tacagtaggt
      781 ataccaagat ttgttattgt tacaacatat caattcggtc agtatgcaat gttgttctta
      841 gcgcctactg gacttgtaat ggcaacactt caaatgttaa acgtgcgcta ctcacactgg
      901 ttacgtttcg tatggcctgt tgtcgcgttt gttttaatat ttggtggagg cttactcatt
      961 acacaagttt taatatattc ttaattttgt tacttttaac aggttagtat aaattaatag
     1021 tctaacctgt tttaatt
//
LOCUS       DANBWH010000373         1033 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.384, whole genome shotgun sequence.
ACCESSION   DANBWH010000373 DANBWH010000000
VERSION     DANBWH010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.384"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..>1033
                     /locus_tag="PPY92_002803"
     CDS             <1..>1033
                     /locus_tag="PPY92_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HDJ2626725.1"
                     /translation="IIFENFSKKCTYWVTLIISQILLTTSVLWILNYTGNLDLFILRL
                     VLLSCLFILTISVINLWTFLMLLNLNIANMIKGKQHFKTIRFINTVCKSILLVLIASV
                     MIENTSVIKDLNKIKETEKYWNVLDDYYTIEFAPYHETKQSLIDNMLRSEQLVKASEA
                     ENNAILFKPKGDSVDNDNFSPDEGNVILVNNQFWSIYYKQFQPDIPIKNQKNNVEVII
                     PQKFHAMRNEINQAYHSWFEFVQNKNNKENKLSIQFINKNDYRIFSFDARDSRHLSFI
                     EAPIIVNVQASDLSNDFYYAMISQGGYLFKNYDALVKNIENYHLDGEISGITNYKDSV
                     MEMYHENNLK"
BASE COUNT          391 a          124 c          168 g          350 t
ORIGIN      
        1 aataattttt gaaaattttt caaaaaaatg cacgtattgg gtaacgttaa tcataagtca
       61 aattctttta actacaagtg tactttggat attaaattac acgggtaatt tagatttatt
      121 catattaaga ttagttctgc tttcatgtct tttcatttta acgattagtg ttattaattt
      181 atggactttc ttaatgttac taaatttaaa tattgctaat atgattaaag gtaagcagca
      241 ctttaaaaca attcgtttta ttaatacagt ttgtaaaagt attctcttag tactaatagc
      301 tagtgtaatg atagaaaata ctagtgttat taaagatttg aataaaataa aggaaactga
      361 gaaatattgg aatgtattag atgattatta cacgattgaa tttgcacctt atcacgaaac
      421 gaaacaaagt ttgattgata atatgctgcg atcagaacaa ttagtaaagg ctagtgaagc
      481 agaaaataat gcgattttat tcaaaccaaa gggtgactcc gttgacaatg acaacttttc
      541 gcctgatgag gggaatgtaa tattagtaaa taatcagttc tggtcgattt attacaagca
      601 gtttcaacct gatattccga taaaaaatca aaaaaataat gtcgaagtaa ttattccaca
      661 aaagtttcat gcaatgcgta atgaaatcaa tcaagcatat cattcatggt ttgaatttgt
      721 acaaaataaa aataataaag agaataagtt atctatacag tttatcaaca aaaatgatta
      781 tcgaattttt tcatttgatg cacgagatag tcgccatttg tcatttatag aggcgccaat
      841 cattgtgaat gttcaggcat cagatttatc gaatgatttt tattatgcca tgatcagtca
      901 aggcgggtat ttattcaaaa attatgacgc gctagtaaaa aatattgaaa attatcatct
      961 tgatggggaa atcagtggaa taaccaatta taaagatagc gtgatggaaa tgtatcatga
     1021 aaacaatttg aaa
//
LOCUS       DANBWH010000374         1032 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.385, whole genome shotgun sequence.
ACCESSION   DANBWH010000374 DANBWH010000000
VERSION     DANBWH010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.385"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            330..731
                     /locus_tag="PPY92_002804"
     CDS             330..731
                     /locus_tag="PPY92_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl peptide receptor-like 1 inhibitory
                     protein"
                     /protein_id="HDJ2626726.1"
                     /translation="MKKNITKTIIASTVIAAGLLTQTNDAKAFFSYEWKGLEIAKNLA
                     DQAKKDDERIDKLMKESDKNLTPYKAETVNDLYLIVKKLSQGDVKKAVVRIKDGGPRD
                     YYTFDLTRPLEENRKNIKVVKNGEIDSIYWD"
     gene            691..783
                     /locus_tag="PPY92_002805"
                     /pseudo
     CDS             691..783
                     /locus_tag="PPY92_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499655.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          388 a          158 c          142 g          344 t
ORIGIN      
        1 ctatatgtat aacaatttgg agtgtttgtc tacccaacat tgcaaacaaa atgcaaagaa
       61 atatatatcg ttttaacaat aaaaattatc caatcatagt tgagtgacgt ttactactat
      121 tattatacta tcaacaaatt caataatacc tcaaataaaa ataataccat caaattcatc
      181 attattcttt ttcgattgca ttaaacctta ttaatttgca cttaatttta attaatgaag
      241 tgttaactgg taaatgcatg aaaccgtttg taaaatagca gtgtactcgc attagtaagt
      301 acaaatttat tttacaggga gaacttatta tgaaaaaaaa tatcacaaaa actattatcg
      361 catctacagt aatcgctgca ggtcttttaa ctcaaactaa tgatgctaaa gctttcttta
      421 gttatgaatg gaaaggttta gaaatcgcaa aaaatttagc agatcaagct aaaaaggacg
      481 atgaacgtat tgataagtta atgaaagagt ctgataaaaa tctaactcct tacaaagctg
      541 aaactgttaa tgatctgtac cttattgtta aaaaattaag ccaaggtgat gtgaagaaag
      601 cagttgtcag aattaaagat ggtggtccta gagattacta tacttttgac ttaactcgtc
      661 ctttagaaga gaacagaaaa aatattaaag ttgttaaaaa cggtgaaatc gactcgattt
      721 attgggatta attaacatac tgaattaaat agttgtacgc caaacgttag aaaacaatgc
      781 taacgtattg gcgtgctttt ttattttaag taacttccaa tttatttagc atctttacga
      841 ctgtttaata aagcacgtat gattaacacg gttgccacta tatccgttac aatttttatg
      901 attgttaata cattcgattt ccttttcaca atagccacct cgcttgttca aaacataaac
      961 aactattgca ttcactttta agtaattatt tatatgattt atcccaataa gctcaccatt
     1021 caaataaaca ca
//
LOCUS       DANBWH010000375          995 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.386, whole genome shotgun sequence.
ACCESSION   DANBWH010000375 DANBWH010000000
VERSION     DANBWH010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.386"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            29..724
                     /locus_tag="PPY92_002806"
     CDS             29..724
                     /locus_tag="PPY92_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDJ2626727.1"
                     /translation="MGVLTIEHLTKKIGNKTILEDVSFKLKRGQIVGLVGANGAGKTT
                     LMKVILGYSSFQSGNFNVINSKDSKSNIGALIENPGIYPFMSGYENLKLLNESKNTQD
                     IDKIVSQLHMDEYIHKKAKTYSLGMKQKLGIAIAFLNEPQFIILDEPMNGLDPKAVRD
                     VRELIVQKSQEGVTFLISSHILSELVKITNSILIINKGKIVTETSEEELKQFKDNDLE
                     NVLLEIIEREDQA"
     gene            726..>995
                     /locus_tag="PPY92_002807"
     CDS             726..>995
                     /locus_tag="PPY92_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDJ2626728.1"
                     /translation="MGTLIKQECFKLFKKKSTFIAPIVFILLMVAQGYIATKYNEIFT
                     PQESFTSAYNGFSWFAFLLIIQASTIISMEFHYGTIKNLLYREYSR"
BASE COUNT          382 a          138 c          163 g          312 t
ORIGIN      
        1 attatttaat agcaaaggag atagtgatat gggtgtttta acaatagaac atttaacaaa
       61 gaagataggc aacaaaacga ttctcgaaga tgtatcattt aagctgaaac gcggacaaat
      121 agttggtctc gttggagcga atggtgcagg taaaacaact ttaatgaaag ttatattagg
      181 ttactctagt ttccaaagcg gaaattttaa tgttattaac agcaaagaca gcaaaagcaa
      241 tatcggtgca ttgattgaaa atccaggaat atatcctttt atgtctggat atgaaaactt
      301 gaagttattg aatgaatcaa aaaacactca agatatcgat aaaattgtct cacaacttca
      361 tatggatgaa tacattcata aaaaagctaa aacgtattct cttggtatga aacaaaaatt
      421 aggaattgct atagcatttt taaatgaacc tcaattcatt atcttagatg aaccaatgaa
      481 cggcttagat ccaaaagctg tgcgagatgt acgtgaattg attgtccaaa agtcgcaaga
      541 aggtgttact ttcttaattt cgagtcatat tttaagtgag ttagttaaaa tcacaaactc
      601 tatccttatt attaacaaag gtaaaattgt tacagaaaca tcggaagaag aacttaaaca
      661 atttaaagat aatgatttag aaaatgtatt actagaaatc atagaaaggg aggaccaagc
      721 ataaaatggg aactttaatt aaacaagaat gtttcaaatt atttaaaaag aaatcaactt
      781 ttatcgcacc tattgtcttc attctactaa tggttgctca aggttatatc gctacaaaat
      841 acaatgaaat ttttacgcca caggaatctt tcacatctgc ttataatggt ttttcatggt
      901 ttgcattttt attaattatt caagcaagta caatcatttc aatggaattt cattacggta
      961 cgattaaaaa tttactctat cgtgaatatt caaga
//
LOCUS       DANBWH010000376          964 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.387, whole genome shotgun sequence.
ACCESSION   DANBWH010000376 DANBWH010000000
VERSION     DANBWH010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.387"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..>964
                     /locus_tag="PPY92_002808"
     rRNA            <1..>964
                     /locus_tag="PPY92_002808"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          285 a          180 c          278 g          221 t
ORIGIN      
        1 attagacgaa tcatctggaa agatgaatca aagaaggtaa taatcctgta gtcgaaaatg
       61 ttgtctctct tgagtggatc ctgagtacga cggagcacgt gaaattccgt cggaatctgg
      121 gaggaccatc tcctaaggct aaatactctc tagtgaccga tagtgaacca gtaccgtgag
      181 ggaaaggtga aaagcacccc ggaaggggag tgaaatagaa cctgaaaccg tgtgcttaca
      241 agtagtcaga gcccgttaat gggtgatggc gtgccttttg tagaatgaac cggcgagtta
      301 cgatttgatg caaggttaag cagtaaatgt ggagccgtag cgaaagcgag tctgaatagg
      361 gcgtttagta tttggtcgta gacccgaaac caggtgatct acccttggtc aggttgaagt
      421 tcaggtaaca ctgaatggag gaccgaaccg acttacgttg aaaagtgagc ggatgaactg
      481 agggtagcgg agaaattcca atcgaacctg gagatagctg gttctctccg aaatagcttt
      541 agggctagcc tcaagtgatg attattggag gtagagcact gtttggacga ggggcccctc
      601 tcgggttacc gaattcagac aaactccgaa tgccaattaa tttaacttgg gagtcagaac
      661 atgggtgata aggtccgtgt tcgaaaggga aacagcccag accaccagct aaggtcccaa
      721 aatatatgtt aagtggaaaa ggatgtggcg ttgcccagac aactaggatg ttggcttaga
      781 agcagccatc atttaaagag tgcgtaatag ctcactagtc gagtgacact gcgccgaaaa
      841 tgtaccgggg ctaaacatat taccgaagct gtggattgtc ctttggacaa tggtaggaga
      901 gcgttctaag ggcgttgaag catgatcgta aggacatgtg gagcgcttag aagtgagaat
      961 gccg
//
LOCUS       DANBWH010000377          958 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.388, whole genome shotgun sequence.
ACCESSION   DANBWH010000377 DANBWH010000000
VERSION     DANBWH010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.388"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            129..620
                     /locus_tag="PPY92_002809"
     CDS             129..620
                     /locus_tag="PPY92_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HDJ2626729.1"
                     /translation="MVSDFESNDKITEVELLMHYNPKVINSKIKAMRSQIESLYHLNM
                     NHVITNDNDMLVSVSYPLDKLVLYIIEEKDKLEYYMKTAQDRLNLFKDIIKNYSENEQ
                     QDVMRYMLSSGKVKNEGVIERLKVDIYKVESRKRQERQNQREELHRIEFNKHLEQVKK
                     ELS"
     gene            620..772
                     /locus_tag="PPY92_002810"
                     /pseudo
     CDS             620..772
                     /locus_tag="PPY92_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
BASE COUNT          365 a          113 c          187 g          293 t
ORIGIN      
        1 ttcgacgggt accggcgggg gcccttcgct tgcaacgcgg ataaactttt atgaaagggg
       61 gataacttga tttatccctt tttcagatac ttataaatat taaattcgat atgtaaaggt
      121 gtggttattt ggtatctgac tttgaaagca atgataaaat cactgaagta gaacttttaa
      181 tgcattacaa tccgaaagtc attaatagca aaattaaagc gatgagatca caaatagaaa
      241 gcttatatca cttgaacatg aaccatgtga tcacgaatga taacgatatg ctggtatctg
      301 tatcatatcc attagataaa ttagtacttt atattataga agagaaagat aaacttgaat
      361 attacatgaa aacagctcag gataggctaa atttgtttaa agatatcatc aaaaactatt
      421 ctgaaaatga acaacaggat gttatgaggt acatgttaag tagtgggaaa gttaaaaatg
      481 aaggagtcat tgaacgttta aaagtagata tttataaagt tgaaagtaga aagcgtcaag
      541 aacgacaaaa ccagcgagaa gaattacatc gaatagagtt taataagcat ttagaacaag
      601 ttaaaaaaga actttcatag ataagcatga tattaacggg aatgtgccta tattcattaa
      661 tattggtgaa tgggacggag atgatgatga gctagacaaa actgtaaaag atgtatctaa
      721 cgctaatcct aatcatactg tgattgtgga tgatattccg ttagaggatt gaagaaaatg
      781 aagctatgct atttataaat taatactaat tagtttgata ccatagctta tttactgaga
      841 aagtagactt aaatgtaaca acaccagtgt ttattgataa tattggtggg tttgaggagt
      901 agcaataaaa taaaggaggt aattgtgtaa aatatctctt tttgttattt cttattta
//
LOCUS       DANBWH010000378          943 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.389, whole genome shotgun sequence.
ACCESSION   DANBWH010000378 DANBWH010000000
VERSION     DANBWH010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.389"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(165..857)
                     /locus_tag="PPY92_002811"
     CDS             complement(165..857)
                     /locus_tag="PPY92_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDJ2626730.1"
                     /translation="MNYSPKQGVRSHGLPHDPFKSSTVPRPIGWISTVSKDGKDNLAP
                     YSQYQNLTWDPPMVMFAANQSVLGDHERKDTVKNAEETGWFVWNMATYDLREAVNLSS
                     KALPPEEDEFEYAGVTKEACIEAPGYRVKESPVHFECEYVQTIRIPTGDPVSTVDIVI
                     GRVAQVHIDDKVILDNGKLDIKSIKPIARLGYYDYTVVNEIFEMKAPAASKEELAGLE
                     GRNFDNQSDEKR"
BASE COUNT          288 a          197 c          129 g          329 t
ORIGIN      
        1 aaacctagtg taatatatgt gtaattctaa aagattcttc tttaaaaata taaataccac
       61 gacatattgc tttaacattt tcatttataa agcgaaaaaa tgcatcgcta ctaagttgaa
      121 tatttagtaa caatgcattg aattcactaa aatgattaaa ttacttatct cttttcatct
      181 gattgattat cgaaatttct tccttctaaa cctgctaact cttctttaga agctgcaggt
      241 gctttcattt caaatatctc attcactact gtgtaatcgt aatatcctaa tctggcaata
      301 ggtttaatcg acttaatgtc caatttacca ttatcaagaa taaccttatc gtcaatatga
      361 acttgggcaa ctcttcctat aacaatatct acggtagata ctgggtctcc tgtcggaata
      421 cgaatcgttt gaacgtactc acattcaaaa tgtactggcg attctttgac tcgataacct
      481 ggtgcttcta tacatgcttc tttcgtcaca cctgcatatt caaattcatc ctcttcaggt
      541 ggtagtgcct tcgacgataa attaacagct tctcttaaat catacgtggc catgttccat
      601 acaaaccacc ctgtctcttc tgcatttttt actgtatctt tacgctcatg atcaccaaga
      661 acagattgat ttgctgcgaa cataaccata ggcggatccc aagttaagtt ttgatactga
      721 ctataaggcg ctaaattatc tttcccatct ttcgatacag tagaaatcca acctattgga
      781 cgaggtactg tactactttt aaatgggtcg tgcggtaaac catgacttct tacaccttgt
      841 tttggcgaat aattcatact atcttcaccc cttataagta attacattta aggttacgcc
      901 ctctttacat aagcgtctaa tataaataaa caatttattt ata
//
LOCUS       DANBWH010000379          941 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.390, whole genome shotgun sequence.
ACCESSION   DANBWH010000379 DANBWH010000000
VERSION     DANBWH010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.390"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..929)
                     /locus_tag="PPY92_002812"
     CDS             complement(<1..929)
                     /locus_tag="PPY92_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HDJ2626731.1"
                     /translation="MIYSVGNRLGNKLTGIEKAMINRHNLFKNHSITSKLIFASWSPR
                     LHFNASLFQIEDDDILSLYDVLQDSINIKSIHKDWITHWTKECNYTLKFIENTNDIKI
                     YEKSNYRMYVHFNDSNYQHLDYINHFDVNQRKIRRDLYDSRGFLSCSRTLTTNQKVVC
                     EHYYTPEGTIKFQKFFNPELENSQSQLIIYNTDSQLKYFNNDQELLAFAIEKLYKNGD
                     IFLSDKNINTAPIFNNTSETIPVLAVLHSTHVKNIDMVYESDIKNTYKHVFNNLNRYS
                     GIIVSTKQQQLDISARINNEIPVHTIPVGYIDE"
BASE COUNT          301 a          135 c          127 g          378 t
ORIGIN      
        1 tgttcatcaa tatatcctac tggtatagta tgaacaggga tttcattatt tattcgagct
       61 gatatatcca attgttgctg tttagtcgat acaattatcc cagagtatct atttagattg
      121 ttaaaaacat gtttataagt atttttgatg tccgattcat ataccatgtc aatattcttg
      181 acatgagtac tatgcaaaac agctaaaaca ggtattgttt cactagtatt attgaatatt
      241 ggtgcagtat ttatattttt atcactcagg aaaatatctc catttttata caatttttca
      301 attgcaaatg ctagtaattc ctgatcatta ttaaaatact taagttgaga atcggtgtta
      361 taaataataa gttgtgattg tgaattttca agttctggat taaaaaattt ttggaacttt
      421 atagtacctt caggagtgta ataatgttca caaactactt tttgatttgt tgttaaagtt
      481 cttgaacaac ttaaaaagcc tcttgaatca tatagatctc gacgtatttt acgttgattt
      541 acatcaaaat gatttatgta atccaaatgt tggtaattac tatcattaaa atggacatac
      601 attctatagt tgcttttttc atatatcttt atatcattgg tattttcaat aaattttagc
      661 gtataattac actctttagt ccaatgagtg atccagtcct tatggatgct ttttatattt
      721 atactgtctt gtagcacatc atataagctt aatatatcgt catcttcaat ttgaaataga
      781 cttgcattaa aatgtaaacg aggtgaccac gatgcaaaaa tcaatttact agttatcgag
      841 tgatttttaa ataagttgtg tctattaatc atcgcttttt caattccggt taatttattt
      901 cctaacctat tccctactga atatatcatt ttaattcaac c
//
LOCUS       DANBWH010000380          932 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.391, whole genome shotgun sequence.
ACCESSION   DANBWH010000380 DANBWH010000000
VERSION     DANBWH010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.391"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            298..435
                     /locus_tag="PPY92_002813"
     CDS             298..435
                     /locus_tag="PPY92_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001788572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(glycerol-phosphate)
                     alpha-glucosyltransferase"
                     /protein_id="HDJ2626732.1"
                     /translation="MADKILKLVNNDVLAEEFGSKARENIIEKYSTESILEKWLNLFN
                     S"
     gene            complement(524..880)
                     /locus_tag="PPY92_002814"
     CDS             complement(524..880)
                     /locus_tag="PPY92_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDJ2626733.1"
                     /translation="MKLGMLLIRWMLATSFLVHGTLKFVDLDGTIHFLGSLGLPPTIA
                     VLMSIGEVVGGLALIIGFLTPYVNVFYICVMLGSIFTLKLTRGYVSGYEFEIIHIVMN
                     LACISSYSWKKWLQFY"
BASE COUNT          385 a          139 c          132 g          276 t
ORIGIN      
        1 cacctgggaa gaaccataat gctgaagctg tgtaccaaat cattggtaac gccataataa
       61 taaaagatat aagacaagaa aggataccaa taatattata agttctagtc aaaattacac
      121 ctccaatttt cattttaaaa tgacatgcgt aaaacaatca ttttaaatac atcatacaat
      181 aaaattaaat tgtgcggtag aagaatttga aattaaattg aattggcttg attaaaaggt
      241 gattttatag aagttaataa aaatggttat ttaatagaaa aacataatat taaagacatg
      301 gctgataaaa tactaaagct tgttaataat gatgttttag cagaggagtt tggttcgaaa
      361 gcgagagaaa acattataga aaaatattca acggaatcaa tattagaaaa atggttaaat
      421 cttttcaata gctaaatgaa aaaatcctag gtattcatga gtacctagga tttttacgca
      481 tgatatatac aaaaaaagag aggatagtcc tctcaaataa tagttaataa aattgtagcc
      541 atttcttcca gctgtaacta ctaatacaag ctaagttcat tacgatatgt atgatttcaa
      601 attcataacc acttacatat cctcttgtaa gtttaagtgt gaagattgag cctaacataa
      661 cacaaatata aaaaacatta acataaggcg ttaaaaagcc gataataagt gctaaacctc
      721 caacaacttc accaattgac atcaatactg cgatggtagg tggtaaacct aaagagccta
      781 agaagtgaat tgttccatct aaatcaacaa atttcaaagt accatgcact aagaatgatg
      841 ttgctaacat ccatctaatt aaaagcatac ctaatttcat catgtcacct ccaacattca
      901 tatttttaaa acatagtcca aattatattt gt
//
LOCUS       DANBWH010000381          924 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.392, whole genome shotgun sequence.
ACCESSION   DANBWH010000381 DANBWH010000000
VERSION     DANBWH010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.392"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            11..763
                     /gene="sarS"
                     /locus_tag="PPY92_002815"
     CDS             11..763
                     /gene="sarS"
                     /locus_tag="PPY92_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarS"
                     /protein_id="HDJ2626734.1"
                     /translation="MKYNNHDKIRDFIIIEAYMFRFKKKVKPEVDMTIKEFILLTYLF
                     HQQENTLPFKKIVSDLCYKQSDLVQHIKVLVKHSYISKVRSKIDERNTYISISEEQRE
                     KIAERVTLFDQIIKQFNLADQSESQMIPKDSKEFLNLMMYTMYFKNIIKKHLTLSFVE
                     FTILAIITSQNKNIVLLKDLIETIHHKYPQTVRALNNLKKQGYLIKERSTEDERKILI
                     HMDDAQQDHAEQLLAQVNQLLADKDHLHLVFE"
BASE COUNT          370 a          144 c          115 g          295 t
ORIGIN      
        1 agataaaaca atgaaatata ataaccatga caaaattaga gattttataa tcattgaagc
       61 atatatgttt cgttttaaga aaaaagtcaa gcctgaagtc gatatgacta taaaagaatt
      121 tatattactg acttatttat ttcatcagca agaaaacaca cttccattta agaagattgt
      181 ttcagattta tgttataaac aatcggattt agtacagcat ataaaagtac ttgtgaaaca
      241 ttcatatatt agtaaagttc gaagtaaaat tgatgagcgt aatacttaca tttcaatatc
      301 tgaagaacaa cgagagaaaa ttgcagaacg tgttacattg tttgatcaaa tcattaaaca
      361 atttaacctt gcagatcaaa gtgaatcaca gatgatacca aaagatagta aagaatttct
      421 aaacttgatg atgtatacaa tgtatttcaa gaatattatc aaaaaacatc taacattaag
      481 ttttgtagaa ttcacaattc tagctattat cacttctcaa aataaaaaca tcgttcttct
      541 taaagattta attgaaacaa tccaccataa ataccctcaa actgttagag ctctcaataa
      601 tttaaaaaag caaggctatc taataaaaga acgctcaact gaagatgaaa gaaaaatttt
      661 aattcatatg gatgacgcgc agcaagacca tgctgaacaa ttattagctc aagtgaatca
      721 attattagca gataaagatc atttacatct tgtttttgaa taatatctct attacgcaag
      781 tgtgctgtat tctaaagtgc acttgtgttt tctatttttt aataaaacct cagcacataa
      841 tgaacaactt tctattttct atatcactta aaaccatttc cgaaattaaa cctcagcaca
      901 ttcaaagccc cactttattc ttaa
//
LOCUS       DANBWH010000382          861 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.393, whole genome shotgun sequence.
ACCESSION   DANBWH010000382 DANBWH010000000
VERSION     DANBWH010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.393"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            67..603
                     /gene="amaP"
                     /locus_tag="PPY92_002816"
     CDS             67..603
                     /gene="amaP"
                     /locus_tag="PPY92_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDJ2626735.1"
                     /translation="MKRLKNFILGLLIVVIVGFLLFMYIQDGRITEYQDYFLQFEWFQ
                     PLLISLAALLILIGLILVFSIFKPTHRKPGLYKDFDDGHVYVSRKAVEKTAFDTVAKY
                     DQVRQPNVVAKLYNKKNKSYIDIKTDFFVPNNVQVQSLTEAIRSDIKKNVEYFTEMPV
                     RKLEVNVRDQKTSGPRVL"
     gene            616..855
                     /locus_tag="PPY92_002817"
     CDS             616..855
                     /locus_tag="PPY92_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDJ2626736.1"
                     /translation="MANNHNQNGQDSTQQVINFLKVFKWRIVGFLAFLLIAILFLTLG
                     FWKTVLIIVLCLIGVGIGYMKDRKQDFMNFLNRWS"
BASE COUNT          296 a          123 c          153 g          289 t
ORIGIN      
        1 aaaaactgat gggtacatat ttaatatgtt tttaaattaa ctttaactaa ggaggtgtcg
       61 ttaggggtga aacgacttaa aaactttatc cttggcttat taatagtagt aatcgtgggg
      121 ttcctgttat tcatgtatat ccaagatgga cgtataactg aatatcaaga ctacttcctt
      181 caatttgaat ggttccaacc attacttatt tcacttgctg cactattgat attaataggt
      241 cttatattag tatttagtat cttcaaacct acgcatcgaa aacctggatt atacaaagac
      301 ttcgatgatg gtcatgttta cgtatctcgt aaagctgttg aaaagacggc attcgatacg
      361 gttgcaaaat atgatcaagt aagacaacca aatgtagttg cgaaactata taacaaaaaa
      421 aataaatctt atatcgacat taaaactgac tttttcgtac caaataatgt tcaagttcaa
      481 tctttaactg aagcaattcg ttcggatatt aaaaagaatg tagaatattt cacagaaatg
      541 ccagtacgta agttagaagt taatgtgaga gatcagaaaa cttctggtcc acgagtgttg
      601 taagggaggt atatcatggc taacaatcat aaccaaaacg gacaagactc tacacaacaa
      661 gtgattaatt ttttaaaagt atttaaatgg agaatcgttg gtttcctagc gtttctatta
      721 atcgcgatat tattcttaac gttaggtttt tggaaaacag tacttatcat cgttttatgt
      781 ttaattggtg taggtattgg gtatatgaaa gaccgtaagc aagattttat gaatttttta
      841 aatcgatgga gttaattcat c
//
LOCUS       DANBWH010000383          861 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.394, whole genome shotgun sequence.
ACCESSION   DANBWH010000383 DANBWH010000000
VERSION     DANBWH010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.394"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..861
                     /locus_tag="PPY92_002818"
     CDS             <1..861
                     /locus_tag="PPY92_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDJ2626737.1"
                     /translation="RCVLKNISFDIEQGKCIALIGKNGAGKSTLIDILIGNVNANSGE
                     IFDKDKLLQSENRSIMFQKTMFPDQLKVIEIINLYQSFYENPLPLEEIIELTKFDSSQ
                     LNQFVNKLSGGQQRLLDFVLSLIGQPQLILLDEPTSTMDIEIREYFWSIIENLKEDNR
                     TILYTSHYIEEVERMSDKIILIENGEIILNDSTSHIRTNQQSQITLSDEYIRKLKLDK
                     DDLVIQKNHNGTIKIITSNVNDTILYLQQLHINLDDIEIQKVSIVDSYFNNKKQRGSN
                     YDTKLLENRI"
BASE COUNT          347 a          111 c          125 g          278 t
ORIGIN      
        1 agatgtgttc taaaaaatat ttcgttcgat attgaacaag gtaaatgtat cgctttaatt
       61 ggaaaaaatg gtgctggaaa gtcaacgtta attgatatat taattggtaa tgttaatgct
      121 aattctggtg agatatttga taaagacaag ttattacaaa gtgaaaatcg cagtataatg
      181 ttccaaaaaa cgatgtttcc agatcaatta aaagttattg agattatcaa cttatatcaa
      241 tcattttacg aaaatccatt accattggaa gaaataatag aactgacgaa atttgattct
      301 agtcaactga accaatttgt aaataaactt tctggtggtc aacaacgatt actcgatttt
      361 gtattatctt taatcggaca accacaattg atcttattag atgaaccaac atcgactatg
      421 gatatagaaa ttagagaata tttttggtca attattgaaa atttaaaaga agataatcga
      481 acgatactct atacatcgca ctatattgaa gaagtcgaac gtatgtcaga caaaattatt
      541 ctcattgaaa atggagaaat aatacttaat gattcaacgt cacatattag aaccaatcag
      601 caatctcaga ttacgttatc cgatgaatat ataagaaagt taaaactaga taaagatgat
      661 ttagttattc aaaaaaatca taatggcact atcaaaatta ttacttcaaa tgtaaatgat
      721 acgattttat atcttcaaca acttcatatt aatttggatg atattgaaat acaaaaagtc
      781 tcaattgttg attcatactt caacaataaa aagcaaaggg gatctaatta tgatactaag
      841 ttacttgaaa atcgaattta a
//
LOCUS       DANBWH010000384          850 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.395, whole genome shotgun sequence.
ACCESSION   DANBWH010000384 DANBWH010000000
VERSION     DANBWH010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.395"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(78..593)
                     /locus_tag="PPY92_002819"
     CDS             complement(78..593)
                     /locus_tag="PPY92_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HDJ2626738.1"
                     /translation="MKINTEFKGNNIPYEYAAGADISDLINGNPIKSFPFEITELPKD
                     TKYLAWSLIDYDAIPVCGFAWIHWSVANVSITGDSISIKADLSRTKGDYVQGKNSFTS
                     GLLAEDFSEIENHYVGPTPPDKDHQYELTVYALDHSLNLKNGFYLNEFLKEVNQHKID
                     QTSINLIGRKI"
     gene            complement(571..>850)
                     /locus_tag="PPY92_002820"
     CDS             complement(571..>850)
                     /locus_tag="PPY92_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626739.1"
                     /translation="VTISMIFLGISSGLTRPQTINKLSSSSNLRVMLNYAETLYFIFN
                     IAFLLIGGYLYSIGTIQYLMLFMSLLTFIYLLTLFYLRRDQHENQHRI"
BASE COUNT          294 a          131 c          106 g          319 t
ORIGIN      
        1 tacctaatat ataaattaaa tctcttgttt ctttgtactt ttaattgaac aattttatat
       61 tgatgagata tttagcatta aatttttctt cctataaggt taatacttgt ttgatcaatt
      121 ttatgttgat ttacttcttt taaaaattca ttcaagtaga acccattctt caaatttaaa
      181 gaatgatcta acgcataaac tgttaattca tattgatgat ctttatcagg tggtgttggt
      241 cctacatagt gattttctat ttctgaaaaa tcttcagcca acaacccact agtaaagcta
      301 tttttacctt gtacatagtc accctttgtt cttgataaat ctgcttttat agaaattgaa
      361 tcaccagtga tacttacatt agcaacgctc caatgaatcc atgcaaatcc gcaaactggt
      421 attgcatcat aatctatcag agaccatgca agatatttag tatcttttgg aagttctgtt
      481 atttcaaacg ggaatgattt aattggattg ccatttatta aatcacttat atctgcgcct
      541 gccgcatatt catatggtat attatttcct ttaaattctg tgttgatttt catgttgatc
      601 tctccttaaa taaaataatg ttagtaaata tataaacgtc aataatgaca taaataacat
      661 taaatattga atagttccaa ttgaatatag ataaccacca ataagtaaaa atgcgatatt
      721 aaaaataaaa tataacgttt ctgcataatt aagcatcact cttaagttac tactgctaga
      781 aagtttattt atagtttgtg gacgagttaa ccctgaagaa atacctaaaa atatcataga
      841 aatagtaact
//
LOCUS       DANBWH010000385          845 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.396, whole genome shotgun sequence.
ACCESSION   DANBWH010000385 DANBWH010000000
VERSION     DANBWH010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.396"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..836
                     /locus_tag="PPY92_002821"
     CDS             <1..836
                     /locus_tag="PPY92_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDJ2626740.1"
                     /translation="GQYLLSRKGKRKIFLAQSGGSREGLNFKEIANLKIFTPTIFEEQ
                     QKIGQFFSKLDQQIELEEQKLELLQQQKKCYIQKIFSQELRFKDEEGNYYKGWNKKQL
                     KDVLEFSNKRTINENEYPVLTSSRQGLILQSDYYKDRKTFAESNIGYFILPKNHITYR
                     SRSDDGIFKFNLNLMIDVGIISKYYPVFKGIDANQYYLTLHLNYQLKKEYIKYATGTS
                     QLVLSQKDLQNIKTKLPSYEEQQKIGDFFSEIDRLVEKQSSKVGRLKVRKKELLQKMF
                     V"
BASE COUNT          372 a          115 c          122 g          236 t
ORIGIN      
        1 ttggacagta tctattatca agaaaaggta aaagaaaaat tttccttgca caaagtggag
       61 gtagtcgaga aggactaaac ttcaaagaaa ttgctaattt aaaaatcttc accccaacta
      121 tatttgaaga acaacaaaaa ataggacaat tcttcagcaa acttgaccaa caaattgaat
      181 tagaagaaca aaaacttgaa ttacttcaac aacagaaaaa atgctatata cagaaaatct
      241 tctcacaaga attacgattc aaagatgaag aaggtaatta ctataaagga tggaacaaaa
      301 agcaattaaa agatgtatta gaatttagta ataaaagaac tattaatgaa aatgaatatc
      361 ctgttttaac atcgtcaaga caaggtttaa tacttcagtc agactactat aaagatagga
      421 aaacttttgc agagagtaat attgggtatt tcatactccc taaaaatcat ataacatacc
      481 gttcaagaag cgacgatgga atttttaagt ttaatttaaa tctaatgatt gatgtaggta
      541 ttattagtaa atattaccct gtctttaaag ggatagatgc aaatcaatat tatttaacat
      601 tacacttaaa ctatcaactg aaaaaagaat atattaaata tgcaactggt acatcacaat
      661 tggtactctc acaaaaagac ttgcaaaaca taaagactaa attgccatct tatgaagaac
      721 aacaaaaaat cggtgatttt ttcagtgaaa tagatagatt ggttgaaaaa caatcttcaa
      781 aagtcggacg attaaaagta cgtaaaaaag aactattaca aaaaatgttt gtttaatcca
      841 gataa
//
LOCUS       DANBWH010000386          841 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.397, whole genome shotgun sequence.
ACCESSION   DANBWH010000386 DANBWH010000000
VERSION     DANBWH010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.397"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..701
                     /gene="seo"
                     /locus_tag="PPY92_002822"
     CDS             <1..701
                     /gene="seo"
                     /locus_tag="PPY92_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="staphylococcal enterotoxin type O"
                     /protein_id="HDJ2626741.1"
                     /translation="NEEDPKIESLCKKSSVDPIALHNINDDYINNRFTTVKSIVSTTE
                     KFLDFDLLFKSINWLDGISAEFKDLKVEFSSSAISKEFLGKTVDIYGVYYKAHCHGEH
                     QVDTACTYGGVTPHENNKLSEPKNIGVAVYKDNVNVNTFIVTTDKKKVTAQELDIKVR
                     TKLNNAYKLYDRMTSDVQKGYIKFHSHSEHKESFYYDLFYIKGNLPDQYLQIYNDNKT
                     IDSSDYHIDVYLFT"
BASE COUNT          339 a           93 c          128 g          281 t
ORIGIN      
        1 caaatgaaga agatcctaaa atagagagtt tgtgtaagaa gtcaagtgta gaccctattg
       61 ctttacataa tattaatgat gattatataa ataatcgatt tacgacagta aaatcaattg
      121 tatcaactac agaaaaattc ttagacttcg atttattatt taaaagtatt aattggttag
      181 atggaatatc tgctgaattt aaagatttaa aagtggaatt tagctcatca gcgatttcta
      241 aagaatttct aggaaagact gttgatattt atggtgttta ctataaagca cattgtcatg
      301 gtgagcatca agtggatact gcctgtacat atggtggggt aacacctcat gaaaataata
      361 aattaagcga gcctaaaaat ataggagtag ctgtgtataa ggataatgta aatgttaata
      421 catttatcgt tactacagat aaaaagaaag ttactgcaca agaacttgat attaaagtaa
      481 gaacaaaatt aaataatgca tataaattgt atgacagaat gactagtgat gtacaaaaag
      541 gttatattaa atttcattct cattcggagc ataaagaatc attttattat gatttatttt
      601 atattaaagg aaatttacca gatcaatatt tgcaaattta taatgataat aaaacaatag
      661 attcatcaga ctatcatatt gatgtttatt tatttacata acaatctgaa agttgatgga
      721 tatcaactta caatcctata aagaactata gatattaaca tatttttaca aagtagctta
      781 gtaaatgatg aagaacgaat agtgattcaa atctaacttt attactataa aaagctttct
      841 a
//
LOCUS       DANBWH010000387          822 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.398, whole genome shotgun sequence.
ACCESSION   DANBWH010000387 DANBWH010000000
VERSION     DANBWH010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.398"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            87..614
                     /gene="isaB"
                     /locus_tag="PPY92_002823"
     CDS             87..614
                     /gene="isaB"
                     /locus_tag="PPY92_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunodominant staphylococcal antigen IsaB"
                     /protein_id="HDJ2626742.1"
                     /translation="MNKTSKVCVAATLALGTLIGVTVVENSAPTSKQAQAAITPYYTY
                     NGYIGNNANFILDKNFINAIKYDNVKFNGIKLAKTNTIKKVEKYDQTFKGVSAKGNEA
                     SQLQFVVKNNISLKDIQKAYGKDLKKENGKTKEADSGIFYYQNAKKTLGIWFVVDHNR
                     VVEVTVGHTPYKTSK"
BASE COUNT          328 a           89 c          144 g          261 t
ORIGIN      
        1 aatataatca ttagtatttt tatggctgaa aaagttataa taaaagtgta aggaataaaa
       61 tatttgtatg gaaaagagag ataattatga ataaaaccag taaagtttgt gtagcagcaa
      121 cattagcatt gggcacactg attggagtaa cagttgttga aaattcggcg ccaacaagta
      181 aacaggcaca ggcagcaata accccatatt atacttataa tggttatatt ggtaataatg
      241 ctaattttat tctggataag aattttatta acgcgattaa gtatgataat gtgaaattca
      301 atggtattaa attagctaaa acgaatacga taaaaaaagt agagaaatat gatcaaactt
      361 ttaaaggtgt ttctgcaaaa ggaaacgaag caagtcaatt gcaatttgta gttaaaaata
      421 atatttcatt aaaagatatc caaaaagctt atggcaagga cttgaaaaaa gaaaatggta
      481 aaacaaagga agctgatagc ggtatttttt actatcaaaa tgctaaaaag acattaggca
      541 tctggtttgt cgttgatcat aatagagttg tcgaagtaac agttggacat acaccataca
      601 aaacaagtaa ataaaataat ggcatattaa ggctagagtg tgaggagaga taccgcattc
      661 tagtcttttt tattaaataa taacgattat tgcgtcttac atagttgttt gaaattgata
      721 aaaatgttta tttgatcatg attatttaaa tagttatctc acatatttca agcattgatt
      781 aagtatatag agcactattt tgtatttgtt aatattttca ca
//
LOCUS       DANBWH010000388          821 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.399, whole genome shotgun sequence.
ACCESSION   DANBWH010000388 DANBWH010000000
VERSION     DANBWH010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.399"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            122..643
                     /locus_tag="PPY92_002824"
     CDS             122..643
                     /locus_tag="PPY92_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001627793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626743.1"
                     /translation="MKRKVLVLTMGVICATQLWHSNHANALVTESVETNFVVKDSDNK
                     NILQTHTEITTEEKFSIVEKSQLNKLKALSNDNYIEYDLHTNQTGIKKGWLYGYSEID
                     SSHFTDRDKRVIRRDHVKEAQSLINDYKDTQSYEDLAKATAKVSTLSQSHQNYLNKQI
                     DKVNNKIEKTEKR"
BASE COUNT          336 a          113 c          128 g          244 t
ORIGIN      
        1 tagtttgttc atcgcaagtt aattattgtt tctaaaatat tggtatataa ttttcaatgg
       61 cgaagaaaac agggtgaaaa agtcggtttt acaatcaaag caaataaggg agcataaata
      121 aatgaaaagg aaagtactag tactaacaat gggtgtaatt tgtgcaactc aattatggca
      181 ttctaatcac gcaaacgcat tagtgacaga gagtgttgaa actaattttg ttgtaaaaga
      241 ttcggataat aaaaatattt tacaaaccca tactgaaatt actactgagg agaaattttc
      301 aatagtagaa aagagtcaat taaataaatt aaaagcacta tctaatgaca actacataga
      361 atatgattta catactaatc aaacaggtat taaaaaaggt tggttatatg gttatagtga
      421 aattgactca tcacatttca cagaccgtga caaacgcgtt attagacgtg atcatgttaa
      481 agaagcacaa agcttaatta acgattataa agatacacaa agctatgaag atctcgctaa
      541 ggcaactgca aaagtaagta cacttagtca gtctcaccaa aattatttaa ataaacaaat
      601 tgataaagtg aataataaaa tagagaaaac tgaaaaacgc taatccaaag taaattataa
      661 gttatacatc tcgtttttaa atgacaattt atccccgtaa atataataaa taatcttttc
      721 aaattccgca tagatataga gacgctaata aacctctttg tctcgatatg atagtctgca
      781 acgattcatg ttgtaggctt tttaatttta caaataaggc t
//
LOCUS       DANBWH010000389          814 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.400, whole genome shotgun sequence.
ACCESSION   DANBWH010000389 DANBWH010000000
VERSION     DANBWH010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.400"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..229)
                     /locus_tag="PPY92_002825"
     CDS             complement(<1..229)
                     /locus_tag="PPY92_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDJ2626744.1"
                     /translation="MNYFDNKIDQFATYLQKRNNLDHIQFLQVRLGMQIIVGNFFKIL
                     VTYSISIFLSVFLFTLVTHLSYMLIRYNAHGA"
     gene            465..599
                     /locus_tag="PPY92_002826"
     CDS             465..599
                     /locus_tag="PPY92_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lysin family phenol-soluble modulin"
                     /protein_id="HDJ2626745.1"
                     /translation="MSCLILRIFILIKEGVISMAQDIISTIGDLVKWIIDTVNKFTKK
                     "
BASE COUNT          314 a          103 c          112 g          285 t
ORIGIN      
        1 gagcaccatg tgcattatag cgtattaaca tataggataa gtgtgtaact aaggtaaaca
       61 aaaatacact taagaatata gaaatactat atgttacaag tattttaaaa aaattaccaa
      121 ctataatttg catccctaaa cgtacttgca aaaattgaat atgatctaag ttatttctct
      181 tttgaagata cgtggcaaac tggtcaattt tgttatcaaa ataattcaat tttacaccac
      241 tctcctcact gttattatac gatttagtac aatcttttat cattatattg cctaactgta
      301 ggaaataaat acttaactgt taaatgtaat ttgtatttaa tattttaaca taaaaaaatt
      361 tacagttaag aataaaaaac gactagttaa gaaaaattgg aaaataaatg cttttagcat
      421 gttttaatat aactagatca cagagatgtg atggaaaata gttgatgagt tgtttaattt
      481 taagaatttt tatcttaatt aaggaaggag tgatttcaat ggcacaagat atcatttcaa
      541 caatcggtga cttagtaaaa tggattatcg acacagtgaa caaattcact aaaaaataag
      601 atgaataatt aattactttc attgtaaatt tgttatcttc gtatagtact aaaagtatga
      661 gttattaagc catcccaact taataaccat gtaaaattag caagtgagta acatttgcta
      721 gtagagttag tttccttgga ctcagtgcta tgtatttttc ttaattatca ttacagataa
      781 ttatttctag catgtaagct atcgtaaaca acat
//
LOCUS       DANBWH010000390          801 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.401, whole genome shotgun sequence.
ACCESSION   DANBWH010000390 DANBWH010000000
VERSION     DANBWH010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.401"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(17..490)
                     /locus_tag="PPY92_002827"
     CDS             complement(17..490)
                     /locus_tag="PPY92_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HDJ2626746.1"
                     /translation="MSKIRSFTILSLLIYLAMMCYTVVTYSKLPTKVPIHYNLAGDAD
                     NFADKWVLLLINSAFIVIWLIFFIAGRYYERFAKWSHYNHTPREIRAIKLFLSTLNLE
                     IMSYMSIFTVLEIWQIQHHHQLNLLWFNMIFIIIIGLTLVIFCLLPTIHKMRDSQ"
BASE COUNT          276 a          125 c          111 g          289 t
ORIGIN      
        1 atgacagttt atatttttat tgagaatctc tcattttgtg aattgtagga agtaaacaaa
       61 atatgacaag cgttaaacca atgataatga taaatatcat attaaaccat agtaaattta
      121 attgatgatg gtgttgtatt tgccaaattt ctaatactgt gaagatagac atatagctca
      181 taatctctaa atttaacgta cttaaaaata atttaatcgc tcgaatttca cgtggtgtat
      241 gattataatg tgaccatttg gcaaatcgtt catagtatct acctgcaatg aaaaatataa
      301 gccaaatcac tataaatgcg ctatttatca aaagcagtac ccatttatca gcaaagttat
      361 cagcatcccc tgctaaatta tagtgaatag gtactttggt tggtaatttt gaataggtca
      421 ctactgtata gcacatcata gctaagtaaa taagtagact taatattgta aaagacctga
      481 ttttagacat tctatcgcct ctttctttac attttatgta taacactctg cctattttac
      541 cttttaatta cattacccca aacgattaaa caatatgtaa tgatactata attgcgtcag
      601 gagtatccgc ttgttaaatg tgcatagctt atatttagct gtttaacatg ccacataatg
      661 attcgaatta ttgttttaat taaacctttt tcacatatgt ataaatttta tagagcgcat
      721 gcttaatatt ttcatttgat tcaaaggcaa aagatagcct tatactcttt tctttactgc
      781 cataaatgta acccggattg a
//
LOCUS       DANBWH010000391          795 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.402, whole genome shotgun sequence.
ACCESSION   DANBWH010000391 DANBWH010000000
VERSION     DANBWH010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.402"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..718
                     /gene="seg"
                     /locus_tag="PPY92_002828"
     CDS             <1..718
                     /gene="seg"
                     /locus_tag="PPY92_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031927817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="staphylococcal enterotoxin type G"
                     /protein_id="HDJ2626747.1"
                     /translation="NYVNAQPDPKLDELNKVSDYKNNKGTMGNVMNLYTSPPVEGRGV
                     INSRQFLSHDLIFPIEYKSYNEVKTELENTELANNYKDKKVDIFGVPYFYTCIIPKSE
                     PDINQNFGGCCMYGGLTFNSSENERDKLITVQVTIDNRQSLGFTITTNKNMVTIQELD
                     YKARHWLTKEKKLYEFDGSAFESGYIKFTEKNNTSFWFDLFPKKELVPFVPYKFLNIY
                     GDNKVVDSKSIKMEVFLNTH"
BASE COUNT          304 a           94 c          128 g          269 t
ORIGIN      
        1 gaattatgtg aatgctcaac ccgatcctaa attagacgaa ctaaataaag taagtgatta
       61 taaaaataat aagggaacta tgggtaatgt aatgaatctt tatacgtctc cacctgttga
      121 aggaagagga gttattaatt ctagacagtt tttatctcat gatttaattt ttccaattga
      181 gtataagagt tataatgagg ttaaaactga attagaaaat acagaattag ctaacaatta
      241 taaagataaa aaagtagaca tttttggcgt tccatatttt tatacatgta taatacctaa
      301 atctgaaccg gatataaacc aaaattttgg aggttgttgt atgtatggtg gtcttacatt
      361 taatagttca gaaaatgaaa gagataaatt aattactgta caggtaacaa tcgacaatag
      421 acaatcactt ggatttacaa taactacaaa taagaatatg gttactattc aggaactaga
      481 ttacaaagca agacactggc tcactaaaga aaaaaagcta tacgagtttg atggttctgc
      541 atttgaatct ggatatataa aatttactga aaagaacaat acaagttttt ggtttgactt
      601 atttcctaaa aaagaactag taccttttgt tccatataag tttttaaata tttacggaga
      661 taataaagtt gttgattcta agagtattaa aatggaagta tttcttaata ctcactgata
      721 agtatatttg atatcactat acaatcataa agtgcgtttt tcgaactttt gttctaagtg
      781 ttttatagat tcatg
//
LOCUS       DANBWH010000392          790 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.403, whole genome shotgun sequence.
ACCESSION   DANBWH010000392 DANBWH010000000
VERSION     DANBWH010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.403"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            14..700
                     /locus_tag="PPY92_002829"
     CDS             14..700
                     /locus_tag="PPY92_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDJ2626748.1"
                     /translation="MTEYLLSAGICMAIVSILLIGMAISNVSKGQYAKRFFFFATSCL
                     VLTLVVVSSLSSSANASQTDNGVNRSGSEDPTVYSATSTKKLHKEPATLIKAIDGDTV
                     KLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENANKIEVEFDKGQR
                     TDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQLLRKSEAQAKKEKLNI
                     WSEDNADSGQ"
BASE COUNT          295 a          110 c          157 g          228 t
ORIGIN      
        1 agaggtgtta gttatgacag aatacttatt aagtgctggc atatgtatgg caattgtttc
       61 aatattactt atagggatgg ctatcagtaa tgtttcgaaa gggcaatacg caaagaggtt
      121 tttctttttc gctactagtt gcttagtgtt aactttagtt gtagtttcaa gtctaagtag
      181 ctcagcaaat gcatcacaaa cagataacgg cgtaaataga agtggttctg aagatccaac
      241 agtatatagt gcaacttcaa ctaaaaaatt acataaagaa cctgcgacat taattaaagc
      301 gattgatggt gatacggtta aattaatgta caaaggtcaa ccaatgacat tcagactatt
      361 attagttgat acacctgaaa caaagcatcc taaaaaaggt gtagagaaat atggccctga
      421 agcaagtgca tttacgaaaa aaatggtaga aaatgcaaat aaaattgaag tcgagtttga
      481 caaaggtcaa agaactgata aatatggacg tggcttagcg tatatttatg ctgatggaaa
      541 aatggtaaac gaagctttag ttcgtcaagg cttggctaaa gttgcttatg tttataaacc
      601 taacaataca catgaacaac ttttaagaaa aagtgaagca caagcgaaaa aagagaaatt
      661 aaatatttgg agcgaagaca acgctgattc aggtcaataa tgctcattgt aaaagtgtca
      721 ctgctgctag tggccctttt ataattttta gatcacgata tgatttatta tcaattcaga
      781 attaaaaaag
//
LOCUS       DANBWH010000393          785 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.404, whole genome shotgun sequence.
ACCESSION   DANBWH010000393 DANBWH010000000
VERSION     DANBWH010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.404"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(43..687)
                     /locus_tag="PPY92_002830"
     CDS             complement(43..687)
                     /locus_tag="PPY92_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDJ2626749.1"
                     /translation="MSQNTNHSYYHQNQHAQSISKVWLYFMYYWIIFGIGCYLGQFLP
                     LSWRQPLSFGLLIIILATLVFERARRFGLIISHIYAVVIGLLSYATFTTYLQNLGPDI
                     FYKNIALAIFAFIAFGIIGYFFVGDASSIGKYLFVTLITLIIASLIGIFLQNPIFYTI
                     ITVVSLLLFLLYTLYDFNRLKRGDYSPREMGFNLFINLLNIIKDILYLANMFRR"
BASE COUNT          312 a          124 c           98 g          251 t
ORIGIN      
        1 tattccaaaa ctttatacaa atttctttat taatttaact gtttatcttc tgaacatatt
       61 agcaaggtaa agtatatcct taataatatt caacaaatta ataaatagat taaatcccat
      121 ttctcttggt gaatagtcac ctctttttaa acgattaaaa tcatacaaag tataaagtag
      181 aaataacaac agactaacga cggtaataat agtgtaaaaa ataggatttt gaagaaaaat
      241 accaattaga ctcgcaataa ttaatgttat taatgtaacg aataaatatt tgcctatact
      301 cgatgcatct ccaacgaaga aataaccaat aataccaaat gctataaatg caaaaattgc
      361 taatgcgata tttttataga aaatatctgg tcctaaattt tgtaaatacg tggtaaacgt
      421 tgcgtatgac aataagccaa tcactacagc gtaaatatgt gaaataatta aaccgaaccg
      481 tctcgctctt tcaaaaacaa gtgttgctaa aataataatc agtaatccaa atgacaaggg
      541 ttgtcgccaa cttaatggta aaaactgacc tagatagcat cctatgccaa atataatcca
      601 ataatacata aaataaagcc acactttact tattgattga gcatgctggt tttgatgata
      661 atatgaatga tttgtatttt gcgacaaaat aattcctcct tctaccgtcg ttttatgcgt
      721 caaattttac agtaaatttt gcttcatcaa aagaaataac ctttaacaag tatagtaatt
      781 ttaca
//
LOCUS       DANBWH010000394          785 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.405, whole genome shotgun sequence.
ACCESSION   DANBWH010000394 DANBWH010000000
VERSION     DANBWH010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.405"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..>785)
                     /locus_tag="PPY92_002831"
     rRNA            complement(<1..>785)
                     /locus_tag="PPY92_002831"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          164 a          236 c          169 g          216 t
ORIGIN      
        1 tgtcaaagga tgtcaagatt tggtaaggtt cttcgcgttg cttcgaatta aaccacatgc
       61 tccaccgctt gtgcgggtcc ccgtcaattc ctttgagttt caaccttgcg gtcgtactcc
      121 ccaggcggag tgcttaatgc gttagctgca gcactaaggg gcggaaaccc cctaacactt
      181 agcactcatc gtttacggcg tggactacca gggtatctaa tcctgtttga tccccacgct
      241 ttcgcacatc agcgtcagtt acagaccaga aagtcgcctt cgccactggt gttcctccat
      301 atctctgcgc atttcaccgc tacacatgga attccacttt cctcttctgc actcaagttt
      361 tccagtttcc aatgaccctc cacggttgag ccgtgggctt tcacatcaga cttaaaaaac
      421 cgcctacgcg cgctttacgc ccaataattc cggataacgc ttgccaccta cgtattaccg
      481 cggctgctgg cacgtagtta gccgtggctt tctgattagg taccgtcaag atgtgcacag
      541 ttacttacac atatgttctt ccctaataac agagttttac gatccgaaga ccttcatcac
      601 tcacgcggcg ttgctccgtc aggctttcgc ccattgcgga agattcccta ctgctgcctc
      661 ccgtaggagt ctggaccgtg tctcagttcc agtgtggccg atcaccctct caggtcggct
      721 atgcatcgtt gccttggtaa gccgttacct taccaactag ctaatgcagc gcggatccat
      781 ctata
//
LOCUS       DANBWH010000395          777 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.406, whole genome shotgun sequence.
ACCESSION   DANBWH010000395 DANBWH010000000
VERSION     DANBWH010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.406"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(10..729)
                     /gene="sem"
                     /locus_tag="PPY92_002832"
     CDS             complement(10..729)
                     /gene="sem"
                     /locus_tag="PPY92_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_058008129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type M"
                     /protein_id="HDJ2626750.1"
                     /translation="MKRILIIVVLLFCYSQNHIATADVGVLNLRNYYGSYPIEDHQSI
                     NPENNHLSHQLVFSMDNSTVTAEFKNVDDVKKFKNHAVDVYGLSYSGYCLKNKYIYGG
                     VTLAGDYLEKSRRIPINLWVNGEHQTISTDKVSTNKKLVTAQEIDTKLRRYLQEEYNI
                     YGFNDTNKGRNYGNKSKFSSGFNAGKILFHLNDGSSFSYDLFDTGTGQAESFLKIYND
                     NKTVETEKFHLDVEISYKDES"
BASE COUNT          253 a          129 c           86 g          309 t
ORIGIN      
        1 tgaaatactt caactttcgt ccttataaga tatttctaca tctaaatgga atttttcagt
       61 ttcgacagtt ttgttgtcat tatatatttt taagaaactt tcagcttgtc ctgttccagt
      121 atcaaataag tcataagaaa atgatgaacc atcattcaaa tgaaataata tttttcctgc
      181 attaaatcca gaactaaatt ttgacttatt accataattt cttcctttat ttgtatcatt
      241 aaagccataa atattatatt cttcttgtag atatcttctt aatttagtat caatttcttg
      301 agctgttact aactttttat tagttgatac tttgtcagta gatatagttt gatgttctcc
      361 attaacccaa agattaatag gaatacgtct agatttctct aaataatcac ctgctaatgt
      421 aactccaccg tatatatatt tgtttttcaa acaatatcca ctataactta gaccatatac
      481 atctacagca tgatttttga atttctttac atcatcaacg ttcttaaatt cagctgttac
      541 tgtcgaatta tccatagaaa aaactaattg atgcgaaaga tgattatttt caggattaat
      601 actttggtgg tcttcaattg gatagctacc ataatagttc ctaagattca aaactccgac
      661 atcagcggtt gcgatatgat tttgcgaata gcaaaacaat aaaacaacaa tgataagtat
      721 tcttttcata ttttatttct cctatattta aacttctcta tctttattat cttttat
//
LOCUS       DANBWH010000396          772 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.407, whole genome shotgun sequence.
ACCESSION   DANBWH010000396 DANBWH010000000
VERSION     DANBWH010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.407"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..598
                     /locus_tag="PPY92_002833"
     CDS             <1..598
                     /locus_tag="PPY92_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDJ2626751.1"
                     /translation="PKFYKEYMYSMTVYSLLSFSLLTFPLDIINEKQNEWRQRLMVTP
                     FTFTSYYISKVVKTMLQFAIAILVIFMVGHFYKGVAMSAVQWLESGIFLWLGASLLIT
                     FGILFSLLNDIQKTSALANIVTIGLAVLGGLWFPINTFPNWLQHVAHVLPSYHLRKLG
                     VDIASNHHINLISFAIILLYVLGSIIAVYCISHFKRAE"
     gene            602..>772
                     /locus_tag="PPY92_002834"
     CDS             602..>772
                     /locus_tag="PPY92_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDJ2626752.1"
                     /translation="MKFLKDTSIAEISSILYLIFPIAGIFFNEVYGPKWLYIISVIVF
                     SLSYLILVIVNNR"
BASE COUNT          245 a           89 c          128 g          310 t
ORIGIN      
        1 acctaaattt tataaagagt atatgtatag tatgacggtt tatagtttgt taagttttag
       61 tttactaact tttccattag atattattaa tgaaaaacaa aatgaatggc gccaaagatt
      121 aatggtaaca ccatttactt ttactagtta ttatatttca aaagtagtga aaactatgct
      181 gcaatttgca atagcgatat tagttatttt tatggttgga catttttata aaggtgttgc
      241 aatgagtgca gttcaatggt tagagtcagg aatattttta tggttaggtg cgtctctatt
      301 aataactttt ggcatattat tttctttgtt aaatgatatt caaaaaacaa gtgctttagc
      361 taatatcgta acaattggtt tagcagtatt aggtggattg tggtttccga taaacacatt
      421 tccaaattgg cttcaacatg ttgctcatgt tttaccgagc tatcatttgc gtaaactagg
      481 tgtagatatt gcttcaaatc atcatatcaa tttaatatca tttgctataa tactcttgta
      541 tgttttaggg agtataatag cagtatattg tattagtcat tttaaaaggg cggaataaaa
      601 tatgaaattt ttaaaagata cttcaattgc tgaaatatcg tctatacttt atctgatttt
      661 tcctattgcc ggtatatttt ttaatgaagt atatggtccc aaatggttgt atattatatc
      721 agtcattgtc ttttcgttgt cgtatcttat attagttata gtaaataata ga
//
LOCUS       DANBWH010000397          762 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.408, whole genome shotgun sequence.
ACCESSION   DANBWH010000397 DANBWH010000000
VERSION     DANBWH010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.408"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(133..741)
                     /locus_tag="PPY92_002835"
     CDS             complement(133..741)
                     /locus_tag="PPY92_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDJ2626753.1"
                     /translation="MDFVNNDTRQIAKNLLGVKVIYQDTTQTYTGYIVETEAYLGLND
                     RAAHGYGGKITPKVTSLYKRGGTIYAHVMHTHLLINFVTKSEGIPEGVLIRAIEPEEG
                     LSAMFRNRGKKGYEVTNGPGKWTKAFNIPRAIDGATLNDCRLSIDTKNRKYPKDIIAS
                     PRIGIPNKGDWTHKSLRYTVKGNPFVSRMRKSDCMFPEDTWK"
BASE COUNT          235 a          149 c          133 g          245 t
ORIGIN      
        1 aaaagaggag atgaatcaaa aaatatgatg gacaaaaaaa caatcaaccc atccgcatgt
       61 tggcggcacg attgattgac ttataaggag atagatttca ttttcatgat agtaatcaat
      121 gaaagatggc atttatttcc aagtatcttc gggaaacata caatctgatt tacgcatgcg
      181 agacacaaat ggattacctt tcactgtgta acgtaaagat ttatgtgtcc aatcaccttt
      241 atttggaata ccgattcgtg gactagcaat aatatcttta ggatatttac gattcttagt
      301 atcaatagac aatctacagt catttaacgt agcgccatcg atagcccgtg gaatgttaaa
      361 tgccttagtc cattttcctg ggccattcgt tacctcgtag cctttcttac ctctgttacg
      421 gaacatagcg gataaacctt cttctggttc aattgcgcgt ataagtacgc cttcaggtat
      481 accttcagat tttgttacaa aattaatgag taaatgcgta tgcatgacat gtgcataaat
      541 tgtaccacca cgtttatata atgacgtgac tttaggtgtt attttaccgc catagccatg
      601 agccgcacga tcattcaaac ctaagtaagc ttccgtttcc acgatgtagc ctgtatacgt
      661 ttgagtggta tcctgataaa tcactttgac acctaataag tttttagcaa tttgtcttgt
      721 atcattatta acgaaatcca taatattctc ctcgcaatta ta
//
LOCUS       DANBWH010000398          757 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.409, whole genome shotgun sequence.
ACCESSION   DANBWH010000398 DANBWH010000000
VERSION     DANBWH010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.409"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            66..728
                     /locus_tag="PPY92_002836"
     CDS             66..728
                     /locus_tag="PPY92_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDJ2626754.1"
                     /translation="MGKEYVVIGLGRFGGSIVRELNALDMDVMAIDHDENRVNEYSDI
                     ATHAVVADTTDEAVMKSLGIRNFDHVIVAIGENIQSSTLTTLILKELGVKKVTAKAQN
                     DYHAKILNKIGADTVVHPERDMGRRIAHNVASASVLDYLELADEHSIVELKATEKMAG
                     QSIIDLDIRAQYGINIIAIKRGKEFIISPNPNINLEIGDILIMIGHDNDLNRFEKNIA
                     TR"
BASE COUNT          265 a           89 c          169 g          234 t
ORIGIN      
        1 atttataata attttaaatt tatattttca atgaattgat gaaaatcatt taatggaggg
       61 cgagtatggg taaagaatat gtagtcatcg gtctaggtcg ttttggaggt agtatcgttc
      121 gtgagttgaa tgcattagac atggatgtaa tggccatcga ccatgatgaa aatagagtga
      181 atgaatatag tgatatcgct actcatgcgg ttgttgcaga cacaacagat gaagcagtta
      241 tgaaaagttt aggtatccgt aactttgatc atgtcattgt ggcaattggt gaaaatattc
      301 aatcaagtac gttgacgact ttaattttaa aagagttagg tgtaaaaaaa gtaactgcta
      361 aagcacaaaa tgattatcat gcaaagattt taaataaaat tggagcagat acggttgtgc
      421 accctgagcg tgatatgggt agacgtattg cgcataatgt tgcgagtgca agtgtacttg
      481 attatcttga gttggcagac gagcattcta ttgtagaatt gaaagcaact gaaaagatgg
      541 cggggcagtc tatcattgat ttagatataa gagcacaata tggaattaac attattgcaa
      601 ttaaacgagg caaagagttc atcatttcac caaatccaaa tattaattta gaaataggtg
      661 atattttaat catgattgga catgataatg atttaaatcg ctttgaaaaa aatattgcga
      721 cgagataatc gggagtggga cagaaatgat attttct
//
LOCUS       DANBWH010000399          754 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.410, whole genome shotgun sequence.
ACCESSION   DANBWH010000399 DANBWH010000000
VERSION     DANBWH010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.410"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(110..198)
                     /locus_tag="PPY92_002837"
     tRNA            complement(110..198)
                     /locus_tag="PPY92_002837"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(161..163),aa:Ser,seq:gga)
     gene            complement(253..324)
                     /locus_tag="PPY92_002838"
     tRNA            complement(253..324)
                     /locus_tag="PPY92_002838"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(289..291),aa:Glu,seq:ttc)
     gene            complement(326..400)
                     /locus_tag="PPY92_002839"
     tRNA            complement(326..400)
                     /locus_tag="PPY92_002839"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(366..368),aa:Asn,seq:gtt)
     gene            complement(418..491)
                     /locus_tag="PPY92_002840"
     tRNA            complement(418..491)
                     /locus_tag="PPY92_002840"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(457..459),aa:Gly,seq:tcc)
     gene            complement(507..582)
                     /locus_tag="PPY92_002841"
     tRNA            complement(507..582)
                     /locus_tag="PPY92_002841"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(547..549),aa:His,seq:gtg)
     gene            complement(596..668)
                     /locus_tag="PPY92_002842"
     tRNA            complement(596..668)
                     /locus_tag="PPY92_002842"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(633..635),aa:Phe,seq:gaa)
     gene            complement(685..754)
                     /locus_tag="PPY92_002843"
     tRNA            complement(685..754)
                     /locus_tag="PPY92_002843"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(725..727),aa:Asp,seq:gtc)
BASE COUNT          234 a          183 c          168 g          169 t
ORIGIN      
        1 atcctactta ctattgaaca aacaaatttt aaattaagtg actcagataa tagacaaaaa
       61 accttaaaaa tagcgcttaa aatgggtaaa accattttaa gcgctataac ggagagtgag
      121 ggattcgaac cctcgagacg cttgtggcgc ctacacactt tccaggcgtg ctccttcggc
      181 caactcggac aactctcctc aataataaat atgtaataaa aaaacagaag cgatatttca
      241 cttctgtttg tatgactcct acgggactcg aacccgtgtt accgccgtga aagggcggtg
      301 tcttaaccgc ttgaccaagg agccatggct ccacaggtag gactcgaacc tacgaccgat
      361 cggttaacag ccgatagctc taccactgag ctactgtgga ataataatag aaaataatgg
      421 agcgggtgat gggaatcgaa cccacaacat cagcttggaa ggctgaggtt ttgccattaa
      481 actacacccg ctaatgtaaa cgattatggg gcggctgaag gggatcgaac cctcgaatgt
      541 cggaaccaca atccgatgtg ttaaccactt caccacaacc gccatgaatt aagaatggtt
      601 caggacagag tcgaactgcc gacacatgga gcttcaatcc attgctctac caactgagct
      661 actgaaccgt aatggtaata ataatggcgg tctcgacggg aatcgaaccc gcgatctcct
      721 gcgtgacagg caggcgtgtt aaccgctaca ctac
//
LOCUS       DANBWH010000400          744 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.411, whole genome shotgun sequence.
ACCESSION   DANBWH010000400 DANBWH010000000
VERSION     DANBWH010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.411"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            42..>744
                     /locus_tag="PPY92_002844"
     CDS             42..>744
                     /locus_tag="PPY92_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDJ2626755.1"
                     /translation="MFTYFKSAFKNAKPQLLITLIYALIAFAVIAVVYLLANFQLAKY
                     AQTIAIYSQFGQKPPVDAYLKVIAVLLIAAVVSLFVLVQIFIGITNVMKRAMSHEKVK
                     FTDLFIAFKKGNYLKSVLIGLVSIAMIIVLSLLTSLLYKLFSPVSEMIMNSVQSSYAD
                     STHLIGIAITTQSIIIIVVLLIKAIITWLLLIPIFNFMTSFVESTNDKVKTHLANGFK
                     AMKNGQKTFFKFFIGI"
BASE COUNT          257 a          108 c          101 g          278 t
ORIGIN      
        1 tttttgttat tgtatttata tgaagaaaag gaggcaccac tttgtttact tactttaaat
       61 cagcatttaa aaatgctaaa ccacaattat taataacatt gatatatgca cttatagcat
      121 ttgctgtaat tgcagttgta tatttacttg ctaatttcca gcttgctaag tatgcacaaa
      181 ctattgcaat ttattcacaa tttgggcaaa aaccaccagt agatgcatat ttaaaagtta
      241 tcgctgtatt acttatagca gctgtagttt cactgtttgt tttagttcaa atttttatcg
      301 gaattacaaa tgttatgaaa cgagcaatga gtcatgaaaa agtaaaattt accgatttat
      361 ttattgcatt taaaaaaggt aattatttaa agagcgttct gatcggtctt gtatctatag
      421 caatgattat cgttctttca cttctgacat cattactata caaattattt tctccagtat
      481 cagaaatgat tatgaattca gtacaatcat catatgcaga tagtacacac ctgattggta
      541 ttgctattac aactcaaagc atcataatca ttgttgtttt attgattaaa gcaattatta
      601 cttggttatt gttaatacct attttcaact ttatgactag tttcgttgaa tcaactaacg
      661 ataaagtaaa aacacactta gctaatggat ttaaagcaat gaaaaatggt caaaaaacat
      721 ttttcaaatt tttcattgga atac
//
LOCUS       DANBWH010000401          741 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.412, whole genome shotgun sequence.
ACCESSION   DANBWH010000401 DANBWH010000000
VERSION     DANBWH010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.412"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..307
                     /gene="lpl1"
                     /locus_tag="PPY92_002845"
     CDS             <1..307
                     /gene="lpl1"
                     /locus_tag="PPY92_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl1"
                     /protein_id="HDJ2626756.1"
                     /translation="FFGQYANLKELRKYNNGDVSINENVPSYDVEYKMSNKDEIVKEL
                     RSRYNISTEKSPILKMHIDGDLKGSSVGYRKLEIDFSKRENSKLSVIEFLSYKPAKK"
     gene            338..>741
                     /locus_tag="PPY92_002846"
     CDS             338..>741
                     /locus_tag="PPY92_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDJ2626757.1"
                     /translation="MGYLKKLALFISVIILGIFIIGCDSSSDTAEKAKEDSKEEQIKK
                     SFAKTLDMYPIKNLEDLYDKEGYRDGEFKKGDKGTWTLLTSFSKSNKPGEIDDEGMVL
                     YLNRNTKKATGYYFVNKIYDDISKNQNEKKYRV"
BASE COUNT          309 a           74 c          144 g          214 t
ORIGIN      
        1 atttttcggt caatatgcaa atcttaaaga attgagaaaa tataataatg gtgatgtttc
       61 aattaatgaa aatgttccta gttatgatgt tgaatacaaa atgagcaata aagatgaaat
      121 agttaaggaa ttaagaagtc gttataatat ttcgactgaa aaatcaccta tattaaaaat
      181 gcatattgat ggggacctga aaggcagttc tgtaggttat agaaagttag aaattgactt
      241 ttcaaaacgt gaaaacagca aattatcagt cattgaattt ttaagttata aaccagcgaa
      301 aaaatagtat tgataaggaa tattaggatg tgaaaaaatg ggatatctaa aaaagcttgc
      361 actgttcata agtgttatta ttttgggcat ttttataata ggttgtgata gttcaagcga
      421 tactgcggaa aaagcaaaag aagattcaaa agaagaacaa atcaaaaaga gctttgcgaa
      481 aacgttagat atgtatccaa ttaagaatct cgaggattta tacgacaaag aaggatatag
      541 agatggtgaa tttaaaaagg gcgataaggg gacttggact ttactcacaa gtttttcaaa
      601 aagtaacaaa ccgggtgaaa tagatgacga aggcatggtt ttatatctaa atagaaatac
      661 caaaaaggca acaggttatt attttgtaaa taaaatttat gatgatatta gcaaaaatca
      721 gaatgagaaa aaataccgtg t
//
LOCUS       DANBWH010000402          737 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.413, whole genome shotgun sequence.
ACCESSION   DANBWH010000402 DANBWH010000000
VERSION     DANBWH010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.413"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            292..666
                     /locus_tag="PPY92_002847"
     CDS             292..666
                     /locus_tag="PPY92_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HDJ2626758.1"
                     /translation="MKRILVVFLMLAIILAGCSNKGEKYQKDIDKVYKEQNQMNKIAS
                     KVQNTIKTDIKQEDSNTHVYKDGKVIVIGIQLYKDREKMYYFAYEIKDGKAEINREID
                     PIKYMKDHKADYEDENVEVEKD"
BASE COUNT          318 a           71 c          125 g          223 t
ORIGIN      
        1 tgtgtgggag gcaattttta gagatacata gcaatgtaaa gatatcaaat agttattgca
       61 taatttatta tgatgacata attttcagta gatagttgta aattaacact gaaatagatg
      121 tttatattaa gaagacttta atatcataat taatcaaaat cttgcgtcaa acatttaaac
      181 gaattttaag ttgataacaa ttggttaaac attgttcaaa catcacaatg ataaagcata
      241 ttatcagtat tgtagtgtgt ggaaaatgac agccatctaa ggagaaaaat gatgaaaaga
      301 atattggtag tatttttaat gttagcaatt atattggcag gttgttctaa taaaggtgaa
      361 aagtatcaaa aagatattga taaagtgtac aaagaacaga atcaaatgaa taaaattgcc
      421 tcgaaagtac aaaacactat taaaacagac attaaacaag aagacagtaa tacacatgtc
      481 tataaagatg gtaaagtcat tgttattggt attcaattat ataaagatcg tgaaaaaatg
      541 tattatttcg catatgaaat aaaagatggt aaggcagaga ttaatagaga aatagaccca
      601 attaagtata tgaaagacca taaagcagat tatgaagatg aaaatgtaga agtggaaaaa
      661 gattaacgta ggctttcttt aaaactttta accagtttca aaaagtaaat gagctttagc
      721 gacaataatt tttaaaa
//
LOCUS       DANBWH010000403          725 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.414, whole genome shotgun sequence.
ACCESSION   DANBWH010000403 DANBWH010000000
VERSION     DANBWH010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.414"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            97..>725
                     /locus_tag="PPY92_002848"
     CDS             97..>725
                     /locus_tag="PPY92_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001628779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coagulase"
                     /protein_id="HDJ2626759.1"
                     /translation="MKNKLLVLSLGALCVSQIWESNHASAVVSGEKNPYVSKALELKD
                     KSNKSNSYENYRDSLESLISSLSFADYEKYEEPEYEKAVKKYQQKFMAEDDALKNFLN
                     EEKKIKNADISRKSNDLLGLTHERYSYIFDTLKKNKQEFLKDIEEIQLKNSDLKDFNN
                     TEQHNADVEINNLENKVLMVGYTFYNTNKDEVEELYSELDLIVGEVQDKS"
BASE COUNT          312 a           56 c          138 g          219 t
ORIGIN      
        1 ttattttaaa aaggtagatg ttatataatt tggcttggcg aaaaaatagg gtgtaaggta
       61 ggttgttaat tagggaaaat taaggagaaa atacagttga aaaataaatt gctagtttta
      121 tcattgggag cattatgtgt atcacaaatt tgggaaagta atcatgcgag tgcagtggtt
      181 tctggggaga agaatccata tgtatcaaaa gctttagaat tgaaagataa aagtaataaa
      241 tccaattctt acgaaaatta tagagatagt ttagaaagtt tgatttcatc attatctttt
      301 gctgattatg aaaaatatga agagccagaa tatgaaaagg ctgtaaaaaa atatcaacaa
      361 aaatttatgg ctgaagatga tgcattaaaa aattttttaa atgaagaaaa gaagataaaa
      421 aatgcagata ttagcagaaa atcgaatgat ttattaggtt taacacatga aagatattct
      481 tatatttttg atacattaaa gaaaaataaa caagagtttt taaaagatat tgaagaaata
      541 caactgaaaa atagtgattt aaaggacttt aacaatacag agcaacataa tgccgacgta
      601 gaaataaaca atttagaaaa taaagtatta atggtagggt atacattcta taatacaaat
      661 aaggacgaag ttgaagaatt atatagtgag ttagatttga ttgttggaga agttcaagat
      721 aagtc
//
LOCUS       DANBWH010000404          697 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.415, whole genome shotgun sequence.
ACCESSION   DANBWH010000404 DANBWH010000000
VERSION     DANBWH010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.415"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..335)
                     /locus_tag="PPY92_002849"
     CDS             complement(<1..335)
                     /locus_tag="PPY92_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626760.1"
                     /translation="METINNLAMATFQLVILFTVAKFISFVKFNLRDYFIIVGIIIPT
                     MFLYYFYGSRAVLIPTFSSIIFLFFKLKYYAIVTILVTMIIMYLSNFATVGLFLTLRK
                     YTTDPAILLP"
     gene            complement(365..508)
                     /locus_tag="PPY92_002850"
     CDS             complement(365..508)
                     /locus_tag="PPY92_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001094921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HDJ2626761.1"
                     /translation="MNTLVNMFFDFIIKLAKAIGIVGGVNACSSLFDEPKVPAELTNL
                     YDK"
     gene            complement(511..>697)
                     /locus_tag="PPY92_002851"
     CDS             complement(511..>697)
                     /locus_tag="PPY92_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDJ2626762.1"
                     /translation="ATKKQPIPIKLVKRKKYLSIIMYLLVLILSLIIHPFYAQFMLLG
                     ILVESITLLPIFFPKED"
BASE COUNT          271 a           93 c           95 g          238 t
ORIGIN      
        1 ggtaagagta ttgcaggatc agtagtgtat tttcttaaag ttaaaaacaa acctactgtt
       61 gcaaaattac tcaaatacat tatgatcatg gtcactaaaa ttgttactat tgcatagtac
      121 tttaatttaa agaataaaaa tatgatagat gaaaaagttg gaatcaatac ggctctactt
      181 ccatagaagt aatacaaaaa catggttgga attataattc caactataat aaaataatcc
      241 cttaaattaa atttaacaaa ggaaatgaat ttggctactg taaaaagtat tactaattga
      301 aaagtggcca tagctaagtt gtttattgtt tccatatgct acacactttc taaaataact
      361 tttactattt gtcgtataaa ttcgttaatt cagcgggtac tttaggttca tcaaataaac
      421 tgctgcaagc gtttacgcca ccgacaattc cgattgcttt agctaattta attataaaat
      481 caaaaaacat attaacaagt gtattcatga ttaatcctcc ttagggaaaa aaataggtaa
      541 taatgtaata gattccacta aaattcctaa taacataaat tgagcataaa atggatgtat
      601 tattaaggat agaatcaaaa ctaataaata cataattata gataaatact ttttacgttt
      661 tactaatttt attggtatag gttgcttttt cgttgct
//
LOCUS       DANBWH010000405          678 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.416, whole genome shotgun sequence.
ACCESSION   DANBWH010000405 DANBWH010000000
VERSION     DANBWH010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.416"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(95..451)
                     /locus_tag="PPY92_002852"
     CDS             complement(95..451)
                     /locus_tag="PPY92_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDJ2626763.1"
                     /translation="MTVDIGRIYDNKDNTDAIRILVDRVWPRGISKRTANLDYWLKDI
                     APSTELRQWFQHDPKLFGAFKEKYEKELRDQDAQKDAFEKLKDIVNQHNHVLLLYAAK
                     DTKHNQAVVLQQLLNT"
BASE COUNT          214 a          118 c          109 g          237 t
ORIGIN      
        1 aagttcaatt aattgtagta attatgcttt ttaaaaataa atattagaaa tgaagttagc
       61 gacatttata gtgattcacg ataaacatat ataactaagt attgagcaac tgctgtagta
      121 ctacagcttg gttatgttta gtatcttttg ctgcatataa caatagaaca tgattatgct
      181 gatttacaat atcctttaat ttttcaaaag catctttttg cgcatcctga tcacgtaatt
      241 ctttttcata tttttcttta aaagctccaa aaagtttagg atcatgttgg aaccattgtc
      301 gcaactcagt agaaggggca atgtctttta accaataatc taggttagca gttcttttcg
      361 aaatacctct cggccagact ctatcgacta ggatacgaat agcgtcggta ttatctttat
      421 tgtcataaat ccgtccaata tctacggtca tcttgtgaac tcctttctta tgaaattcag
      481 tgagcataca tcaatgcatg ttgtggtggg acgaccaaat aaattttgcg aaaatatcat
      541 ctctgtccta ctcccaatta aaaaacagcc atgacaaagt aaagtcatag ctgttttggt
      601 atagatgtca tttattttta cgtttagtta aatacttcaa accaactgca aagaccgtgt
      661 acccggctat ggtttgta
//
LOCUS       DANBWH010000406          678 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.417, whole genome shotgun sequence.
ACCESSION   DANBWH010000406 DANBWH010000000
VERSION     DANBWH010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.417"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(38..>678)
                     /locus_tag="PPY92_002853"
     rRNA            complement(38..>678)
                     /locus_tag="PPY92_002853"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          155 a          197 c          148 g          178 t
ORIGIN      
        1 gtaagtaaaa gtgattttgc ttcgcaaaac atttattttg attaagtctt cgatcgatta
       61 gtattcgtca gctccacatg tcaccatgct tccacctcga acctattaac ctcatcatct
      121 ttgagggatc ttataaccga agttgggaaa tctcatcttg aggggggctt catgcttaga
      181 tgctttcagc acttatcccg tccacacata gctacccagc tatgccgttg gcacgacaac
      241 tggtacacca gaggtatgtc catcccggtc ctctcgtact aaggacagct cctctcaaat
      301 ttcctacgcc cacgacggat agggaccgaa ctgtctcacg acgttctgaa cccagctcgc
      361 gtaccgcttt aatgggcgaa cagcccaacc cttgggaccg actacagccc caggatgcga
      421 tgagccgaca tcgaggtgcc aaacctcccc gtcgatgtga actcttgggg gagataagcc
      481 tgttatcccc ggggtagctt ttatccgttg agcgatggcc cttccatgcg gaaccaccgg
      541 atcactaagt ccgtctttcg accctgctcg acttgtaggt ctcgcagtca agctccctta
      601 tgcctttaca ctctatgaat gatttccaac cattctgagg gaacctttga gcgcctccgt
      661 taccttttag gaggcgac
//
LOCUS       DANBWH010000407          657 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.418, whole genome shotgun sequence.
ACCESSION   DANBWH010000407 DANBWH010000000
VERSION     DANBWH010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.418"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..>657)
                     /locus_tag="PPY92_002854"
     CDS             complement(<1..>657)
                     /locus_tag="PPY92_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626764.1"
                     /translation="VNLLIKPFLVFSTYVIKSKKTFEWYSYFQDIKNEVIIINEKVMQ
                     LLDYISPHTIFMRLNFVNISFVIITIALFIALYYFNKIGFWYRKEDIYLSSTNINFSL
                     PISIKAITTIQLKHWISNKEEVNLHKPFFYISYSIWFFLGCLIYFSQMAYNQLANAVI
                     FILILNTITRDSFSAGTDFFTKSLRFDSDRKSIGLYRMSNTDFKQIYDSKLSLIRFIG
                     "
BASE COUNT          262 a           88 c           74 g          233 t
ORIGIN      
        1 aaaccaataa agcgaattag agataattta gaatcgtaaa tttgtttaaa atcagtgttt
       61 gacatcctat acaaaccgat actttttcta tctgaatcaa aacgtaatga tttagtaaaa
      121 aaatccgtcc ccgcagaaaa actatcacga gttatagtgt ttaatatcaa aataaaaata
      181 actgcattag ctaactgatt atatgccatc tgtgaaaaat agattaaaca ccctaaaaag
      241 aaccatattg aataactaat atagaagaaa ggtttatgta aattaacttc ttctttgtta
      301 cttatccagt gttttagctg tattgttgtt atagctttta ttgaaattgg caacgaaaaa
      361 ttaatatttg ttgaagatag atagatatct tcttttctat accaaaaccc tattttatta
      421 aaataatata acgctataaa taaagctatt gtaataatca caaatgatat attcacaaag
      481 tttaatctca taaaaatagt gtgaggtgat atataatcta ataattgcat aactttttca
      541 ttaataataa tgacttcgtt ctttatatcc tgaaaataac tataccattc aaaagttttc
      601 ttacttttta ttacatatgt cgaaaaaact aaaaagggct ttattaataa atttact
//
LOCUS       DANBWH010000408          656 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.419, whole genome shotgun sequence.
ACCESSION   DANBWH010000408 DANBWH010000000
VERSION     DANBWH010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.419"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(322..522)
                     /locus_tag="PPY92_002855"
     CDS             complement(322..522)
                     /locus_tag="PPY92_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDJ2626765.1"
                     /translation="MNNGTVKWFNAEKGFGFIEREDGSDVFVHFSAIAEDGYKSLEEG
                     QKVEFDIVEGDRGEQAANVVKM"
BASE COUNT          206 a          124 c           84 g          242 t
ORIGIN      
        1 tatgcgatgt ggtatcctat tattgttaaa cattttgagt gtttaacatt acttcttgtt
       61 atcgccatat acagacttta attctcgtca ttattaaagt tctgtattgg cgcttttttt
      121 attcaaaatc aaaagtcgga cagatgaaaa gtaaaaaact tttccatcaa ttcgatttat
      181 tatagaacca ttatatattg ttagatctcc acaatttatt ggacaaaaaa ataacggcta
      241 gtgcttttac cacaccaact gttatcgatt taaagcataa aaaaacagcc ctataataag
      301 gactgtttga ataagttaaa attacatttt aactacgttt gcagcttgct cgccacggtc
      361 gccttcaacg atgtcgaatt caactttttg gccttcttct aatgatttgt atccatcttc
      421 agcgattgct gagaagtgta cgaatacgtc gctaccatct tctctttcga tgaaaccaaa
      481 acctttttct gcattaaacc atttaactgt accgttattc atattgaata cctccgtcgt
      541 gcttttgcac taaatatttt gtaacaaatt cataaatgaa aaggagaata ttctatataa
      601 ataactcaca tttttcttca cgctctttat tacttaactt tcattataca ctttta
//
LOCUS       DANBWH010000409          634 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.420, whole genome shotgun sequence.
ACCESSION   DANBWH010000409 DANBWH010000000
VERSION     DANBWH010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.420"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..>634
                     /locus_tag="PPY92_002856"
     rRNA            <1..>634
                     /locus_tag="PPY92_002856"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          174 a          147 c          181 g          132 t
ORIGIN      
        1 cactgccgag aaaagcctct agatagaaaa taggtgcccg taccgcaaac cgacacaggt
       61 agtcaagatg agaattctaa ggtgagcgag cgaactctcg ttaaggaact cggcaaaatg
      121 accccgtaac ttcgggagaa ggggtgctct ttagggttaa cgcccagaag agccgcagtg
      181 aataggccca agcgactgtt tatcaaaaac acaggtctct gctaaaccgt aaggtgatgt
      241 ataggggctg acgcctgccc ggtgctggaa ggttaagagg agtggttagc ttctgcgaag
      301 ctacgaatcg aagccccagt aaacggcggc cgtaactata acggtcctaa ggtagcgaaa
      361 ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa cgatttgggc actgtctcaa
      421 cgagagactc ggtgaaatca tagtacctgt gaagatgcag gttacccgcg acaggacgga
      481 aagaccccgt ggagctttac tgtagcctga tattgaaatt cggcacagct tgtacaggat
      541 aggtaggagc ctttgaaacg tgagcgctag cttacgtgga ggcgctggtg ggatactacc
      601 ctagctgtgt tggctttcta acccgcacca ctta
//
LOCUS       DANBWH010000410          624 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.421, whole genome shotgun sequence.
ACCESSION   DANBWH010000410 DANBWH010000000
VERSION     DANBWH010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.421"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            88..519
                     /locus_tag="PPY92_002857"
                     /pseudo
     CDS             88..519
                     /locus_tag="PPY92_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086052.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          258 a           75 c           98 g          193 t
ORIGIN      
        1 tatgcatctc acctatcttt ttttgctttt ttcaaataag aaagtattaa aaattaatgc
       61 ctatacaacg ttaaaggagg aacagacatg gctaaacttg atttaaatag tcttgacgat
      121 gagcacgtaa aattattaat aaatgaatta aaatatccag aaactcatat cgatgtaaat
      181 gaataaaaaa caatagttgc tagtcgaata aatgaaaggc aagaaataat aagttttaag
      241 ttaggaataa agtacttctt aacaataaaa agggataaca tagaaaaaga taggttttca
      301 atttcaatca ttttcaaaga tacctatcac accctagtta gaatagatat taacggtggt
      361 actcacgata atccagatgg aacaatcgct ccgaaaagtc atattcacat atataatgat
      421 aagtgtgata aaaaggatag gtttgcttat gaaattaact tgaaagattt ccccgacatc
      481 tataacttgt ataatgtgta tatgtcgttt ttagagtaaa aaattataaa agaccttgaa
      541 taaaggaggg ataatatgac tacgtttgat gctaaaaaat taaaaaaaga atatcttgat
      601 tggtataatc agattttaga gttt
//
LOCUS       DANBWH010000411          608 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.422, whole genome shotgun sequence.
ACCESSION   DANBWH010000411 DANBWH010000000
VERSION     DANBWH010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.422"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            21..>608
                     /locus_tag="PPY92_002858"
     CDS             21..>608
                     /locus_tag="PPY92_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDJ2626766.1"
                     /translation="MNLDWYYTFVILAKTLNYRLASEEINLTIPSIHKQIKNLEQHLN
                     VKLFETYKNQIILTEDGHTFLPIAQSFIEQYESGIKHIQLKKTMFQSKLNVVVSSYIA
                     TFIMPKFLKSFFNEHPFIDVSLHVKNENIEKDINNHTYDIGISRNQPKLREVHSEKVC
                     EGKIVLIAPNKENNHLLTEASLFEKYKIISDNHPEY"
BASE COUNT          234 a           92 c           86 g          196 t
ORIGIN      
        1 tttttgaaag aggtaagttc atgaatttgg attggtacta tacatttgtc attttagcga
       61 agacgttaaa ctatcgatta gctagtgaag aaattaatct cactatacct tctatacata
      121 agcaaattaa aaatttagaa cagcatctca atgtgaaact atttgaaact tataaaaatc
      181 aaattatctt gactgaagat ggtcatacat ttcttccaat tgcgcaaagt tttattgaac
      241 aatatgaaag tggcatcaaa catatccaac ttaaaaaaac gatgtttcaa tcgaaattaa
      301 atgttgtcgt gtcttcatat atcgcgacat tcattatgcc caaattttta aaatctttct
      361 ttaacgagca tccgtttatc gatgtttctc tccatgtcaa aaatgaaaat attgaaaaag
      421 atattaataa tcatacgtat gacatcggga ttagcagaaa tcaacctaaa ctaagagaag
      481 ttcattctga aaaagtatgc gaaggtaaaa ttgtattgat tgctcccaat aaagagaata
      541 atcatcttct aactgaggca tctttatttg aaaaatacaa aataataagt gataaccatc
      601 cagaatat
//
LOCUS       DANBWH010000412          607 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.423, whole genome shotgun sequence.
ACCESSION   DANBWH010000412 DANBWH010000000
VERSION     DANBWH010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.423"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..103
                     /locus_tag="PPY92_002859"
     CDS             <1..103
                     /locus_tag="PPY92_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626767.1"
                     /translation="IFLGNEIIHTLTVLITTLYIVNSRKGIKNDRVE"
     gene            60..284
                     /locus_tag="PPY92_002860"
     CDS             60..284
                     /locus_tag="PPY92_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626768.1"
                     /translation="MLIQERGLKMTELNNIINSLQSLFESESGYKISKNSGVPYQTVQ
                     DLRNGKTKLEDARFRTIIKLYSYYVSLKEH"
BASE COUNT          243 a           87 c          110 g          167 t
ORIGIN      
        1 tatattttta ggaaatgaaa ttatccatac actgactgtt ttaataacaa cattgtatat
       61 tgttaattca agaaagggga ttaaaaatga cagagttgaa taatattata aactctcttc
      121 aatctttgtt tgagtctgaa tcaggctata aaatttcgaa aaattcagga gttccatatc
      181 aaacagtaca agatttaaga aatgggaaaa ccaaactaga agatgctaga tttagaacga
      241 ttattaaact ttatagttac tatgtctcat taaaagaaca ttaatcatgg gacaataaag
      301 tattgctata ataattgaat cattaaatga aggaatagag cctaagacat aataaatcaa
      361 tgtcttaggc tctacaatat tatattggta gtagttgacc gaatgaaaat gaccatgtaa
      421 caagcatttt tggggcccca acacagaagc tgacgaaaag tcagcttaca ataatgtgca
      481 agttggggat gggccccaac acagagaatt tcaaaaagaa attctacaga caatgcaagt
      541 tggcggggcc ccaacataga gaaattcaaa aagaaattct acagacaatg caagttgggg
      601 agtgaca
//
LOCUS       DANBWH010000413          604 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.424, whole genome shotgun sequence.
ACCESSION   DANBWH010000413 DANBWH010000000
VERSION     DANBWH010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.424"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            345..530
                     /locus_tag="PPY92_002861"
     CDS             345..530
                     /locus_tag="PPY92_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626769.1"
                     /translation="MSEYKKKIIELIESNLTGYEISKKTGVSQYVLSQLRQGKREVDN
                     LTLNTTEKLYEYANKVL"
BASE COUNT          269 a           55 c           95 g          185 t
ORIGIN      
        1 ctactggagt tagtagtttg aatattgaaa gactctaatc taataaaaat acataaaagg
       61 tttaacttta gaagtggttg taaacttcaa tgaatattaa acattgaagt ttagtaaaag
      121 gcgtaaggta taaaagcaat cgtttgtctt tgacaaagta attgatgatg aaaagtcgtt
      181 aatatgttga aagaaaaatt gataatatga aatttaacta ttatgaagtg atgatagcca
      241 ttaatgaata gaaaaataat aaaatcattt ataaataaaa actacgttga gttcacagat
      301 ttcgaaaaaa gcagtaaaaa ttgtacagat atggagatga aaaaatgtca gaatacaaga
      361 aaaagataat tgaattaatt gaaagtaatt taacaggata tgaaatttct aaaaaaactg
      421 gagtttctca atatgtactt tcacaattaa gacagggcaa acgcgaagta gataatctaa
      481 ccctgaatac aacagaaaaa ttatatgaat atgccaataa agttttgtaa tataactact
      541 gtataaatta atcaagctat gtttatttga tttaactatt aataaaaatc atatggtaaa
      601 tgga
//
LOCUS       DANBWH010000414          603 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.425, whole genome shotgun sequence.
ACCESSION   DANBWH010000414 DANBWH010000000
VERSION     DANBWH010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.425"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(134..>603)
                     /locus_tag="PPY92_002862"
     CDS             complement(134..>603)
                     /locus_tag="PPY92_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626770.1"
                     /translation="ILSYLVTNRKKPFLFLKTLFMGVVFIFIGYISLAISAVIIYGII
                     QFITIDFGSFFLMGIILILISSIFQLFIVRLLFRKKNVDLTEVVVLEHLIQWFLVYFA
                     IYQAVNEKMDINDINIDNFQSVFFDVSNLNLVILPTLIISWVTIFNYRMRSYK"
BASE COUNT          242 a          101 c           71 g          189 t
ORIGIN      
        1 ttgtgactca tcagcactta tacactagtc aaaaaattca cgcctctact ttttcgaatt
       61 tcaaaaataa aaccacacaa caaaaaaaga caccatttga atgttggtgt ctgatggtat
      121 aatttcatag attttatttg taacttctca ttctatagtt aaatattgtg acccagctaa
      181 tgattaacgt tggtagaatt actaaattca aattagacac gtcaaaaaag acagattgga
      241 aattgtcgat attaatatca ttaatgtcca ttttttcatt tactgcttga tagatcgcaa
      301 agtaaactaa gaaccattga attaaatgct ctaaaacgac aacctctgtc aaatcgacat
      361 tcttttttct aaaaagtaat ctaactataa ataattggaa tatacttgaa atcaagatca
      421 atataatacc cattaagaaa aaactaccaa aatcaattgt gataaattga ataataccat
      481 aaataattac ggcagatatt gccagtgaaa tatatcctat aaagataaat accacaccca
      541 taaagagtgt ctttaagaac agaaaaggct tctttctatt tgtaaccaaa tagctcaata
      601 tac
//
LOCUS       DANBWH010000415          575 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.426, whole genome shotgun sequence.
ACCESSION   DANBWH010000415 DANBWH010000000
VERSION     DANBWH010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.426"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(448..562)
                     /gene="rrf"
                     /locus_tag="PPY92_002863"
     rRNA            complement(448..562)
                     /gene="rrf"
                     /locus_tag="PPY92_002863"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          155 a          124 c           90 g          206 t
ORIGIN      
        1 ttcaataaag tatataaaag agcagtaaga tcattttcaa ttagataccg tcttactgct
       61 cttctctatt tatacaatat tttgttttcg atgatatcat tttaaattta tataaacaca
      121 aaattaaagt tgtacatact tagacaatcg aaagtgtaca ttattaaatt atcatttcca
      181 gttctactct agcggaacgt aagttggcta ccatcctcgc taagaacctt tcttgacttg
      241 tgacaatcgc ttgcttcttt cctcttcttc ggctctcgct tactcattta gctctactaa
      301 actcgttgcg ctcttttctc gtttcgtcag attcaaacgt tttcacttcg tcaagccatt
      361 ttctttgtgt taacttttat tttgacgttt tagacataaa aaaaagagac cttgcggtct
      421 caatgcggct catcgcatcc attttttgcc tggcaacgtt ctactctagc ggaacgtaag
      481 ttcgactacc atcgacgcta aggagcttaa cttctgtgtt cggcatggga acaggtgtga
      541 cctccttgct atagtcacca gacatatgaa tgtaa
//
LOCUS       DANBWH010000416          559 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.428, whole genome shotgun sequence.
ACCESSION   DANBWH010000416 DANBWH010000000
VERSION     DANBWH010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.428"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            44..433
                     /locus_tag="PPY92_002864"
     CDS             44..433
                     /locus_tag="PPY92_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YisL family protein"
                     /protein_id="HDJ2626771.1"
                     /translation="MLHLHILSWVLAIILFIATYLNISKNQGGSPFFKPLHMILRLFM
                     LLTLISGFWILIQSFMNGGANHMLLTLKMLCGVAVVGLMEVSIAKRKRHEQSHKMFWI
                     TMALIIITMVLGVILPLGPISKLFGIG"
BASE COUNT          191 a           71 c          104 g          193 t
ORIGIN      
        1 ctttaattta ataatataaa taattaaagt ggggggaatc agtatgttac atttacatat
       61 attaagttgg gtattagcga ttattttatt tatcgctaca tacttaaaca tttcaaaaaa
      121 tcaaggcgga tcaccatttt tcaaaccgtt gcacatgatt ttacgcttat ttatgctgtt
      181 gacgttaatt tcaggatttt ggatattaat tcagtcattt atgaatggcg gggcaaatca
      241 tatgttgctt acattgaaaa tgctgtgtgg tgttgcagta gttggattga tggaagtgtc
      301 gattgctaaa agaaagagac atgaacaaag tcacaaaatg ttttggataa caatggcatt
      361 aattatcatc acaatggtat taggtgtcat tctaccgtta gggcctatat caaaattatt
      421 cggtattggc taattgataa gacatatagt atatgacata aggcatggac aattattagt
      481 ccaagacctt atgtcttttt tgattctaaa aatgtttaaa cataaaaaaa ggactggata
      541 gtagtaccag tccaaaata
//
LOCUS       DANBWH010000417          554 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.429, whole genome shotgun sequence.
ACCESSION   DANBWH010000417 DANBWH010000000
VERSION     DANBWH010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.429"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..191)
                     /locus_tag="PPY92_002865"
     CDS             complement(<1..191)
                     /locus_tag="PPY92_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDJ2626772.1"
                     /translation="MHKKYFIGTSILIAVFVVIFDQVTKYIIATTMKIGDSFEVIPHF
                     LNITSHRNNGAAWGILSGK"
BASE COUNT          195 a           92 c           68 g          199 t
ORIGIN      
        1 atttttccac tcaatattcc ccatgcagca ccattatttc gatgtgatgt tatgtttaaa
       61 aagtgcggta tcacttcaaa tgaatctcca attttcattg tagtagctat aatatattta
      121 gtaacttggt caaatataac gacaaatact gctattaaaa tggaagtgcc aataaaatat
      181 tttttgtgca ttttcgttcc tccaatcaat cgtccataag acaactcttt atattatagc
      241 ttacacctgc taataaaaaa agtaagcata ttacattaaa tctaatgtta ctcactcaat
      301 acttgataaa ctactatgtt ttgacattaa atatgaactt aattattcat ttatcatatt
      361 taagatgaca ttaaaaatta ggaaagcagg ctggaacata aatccctaaa aagacagtag
      421 taagatattt tctaattaaa aattatctta ctgctgttct ctatttatac aatacttcgt
      481 attgaatggc ttcgctatgc ccatctggca cattactgta aaattctata aatagaattt
      541 ttgatgatgg gtcc
//
LOCUS       DANBWH010000418          547 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.430, whole genome shotgun sequence.
ACCESSION   DANBWH010000418 DANBWH010000000
VERSION     DANBWH010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.430"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..>547
                     /locus_tag="PPY92_002866"
     CDS             <1..>547
                     /locus_tag="PPY92_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDJ2626773.1"
                     /translation="LNIPVSIVRPYKEDLTLYQYKKGQVIYHSTDQIKFVYFLVNGCI
                     LHESSNITGDNYLRLSKDENIFPMNFIFNETPAPYEICTALTDCKILTLPKDLLEYLC
                     RKHNEIFESLFKKLNETIQFQVEYIMALRANSAKERIERILQILCLSIGDDNGEFYEL
                     KQIMTVQLISNLSGLNRKTTGE"
BASE COUNT          219 a           64 c           77 g          187 t
ORIGIN      
        1 ttaaatattc ctgttagtat tgttagacct tataaagagg atttaacact ttatcaatat
       61 aaaaaaggac aagtcatata tcattcaact gatcaaataa aatttgtata ctttttagta
      121 aatggttgta ttttacatga atcttctaat attactggtg acaattattt aagattaagt
      181 aaagacgaaa atatatttcc aatgaacttc atatttaatg aaacccctgc accatatgaa
      241 atatgtacag ctttgacaga ttgtaaaata ttaactttac cgaaagattt acttgagtat
      301 ttatgtagaa agcataatga aatatttgaa agtctcttca agaaacttaa tgagactatt
      361 caatttcaag tagaatatat tatggcgtta agagctaatt cagctaaaga aagaattgaa
      421 agaatactac aaattttatg cctttcaatt ggggatgata atggagaatt ctatgaatta
      481 aaacaaatta tgactgttca attaataagt aatttatctg gacttaacag aaaaactact
      541 ggtgaaa
//
LOCUS       DANBWH010000419          544 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.431, whole genome shotgun sequence.
ACCESSION   DANBWH010000419 DANBWH010000000
VERSION     DANBWH010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.431"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            96..>544
                     /locus_tag="PPY92_002867"
     CDS             96..>544
                     /locus_tag="PPY92_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HDJ2626774.1"
                     /translation="MLKKAKLILIAVLVLSGCSTIENESKKDRNTETNTETKSVPEEM
                     EASKYVGQGFQPPAEKDAIEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVVGSGDGVE
                     VYVHCDDHDIVFNASIPFDKSIIDSDSSLRSEDKGDDMSTLVGTVLSG"
BASE COUNT          215 a           70 c          119 g          140 t
ORIGIN      
        1 ttatatatag gtgaactaaa agatgcaaat aaattaagtg agtataacta tatgcaaaat
       61 gaaacttata aaagcgcata aatgggtgat gagatatgct taaaaaagca aaattaatct
      121 taatagcagt actagtactg tcagggtgct ccacgattga aaatgaatca aaaaaagaca
      181 gaaatacaga aacaaataca gaaacgaaat ctgtaccaga agaaatggaa gcttcaaaat
      241 atgtaggcca aggattccaa ccgcctgcag aaaaagatgc gattgaattt gcgaagaagc
      301 ataaagataa aattgctaaa cgtggcgaac aattttttat ggataacttc ggtctaaaag
      361 ttaaagctac gaatgttgta ggtagtggcg acggcgtaga agtatacgtg cattgtgatg
      421 accacgatat cgtatttaat gcgagtattc catttgacaa atcaattatt gacagtgata
      481 gctcattaag aagtgaggat aaaggcgatg atatgagtac tttagttggt acagtgttga
      541 gtgg
//
LOCUS       DANBWH010000420          541 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.432, whole genome shotgun sequence.
ACCESSION   DANBWH010000420 DANBWH010000000
VERSION     DANBWH010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.432"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..>541
                     /gene="sei"
                     /locus_tag="PPY92_002868"
     CDS             <1..>541
                     /gene="sei"
                     /locus_tag="PPY92_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031927819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="staphylococcal enterotoxin type I"
                     /protein_id="HDJ2626775.1"
                     /translation="KDLTYAQGDIGVGNLRNFYTKHDYIDLKGVTDKNLPIANQLEFS
                     TGTNDLISESNNWDEISKFKGKKLDIFGIDYNGPCKSKYMYGGATLSGQYLNSARKIP
                     INLWVNGKHKTISTDKIATNKKLVTAQEIDVKLRRYLQEEYNIYGHNNTGKGKEYGYK
                     SKFYSGFNNGKVLFHLNNEK"
BASE COUNT          224 a           67 c           84 g          166 t
ORIGIN      
        1 taaagatctt acgtatgctc aaggtgatat tggtgtaggt aacttaagaa atttctatac
       61 aaaacatgat tatatagatt taaaaggcgt cacagataaa aacctaccta ttgcaaatca
      121 actcgaattt tcaacaggta ccaatgattt gatctcagaa tctaataatt gggacgaaat
      181 aagtaaattt aaaggaaaga aactggatat ttttggcatt gattataatg gtccttgtaa
      241 atctaaatac atgtatggag gggccacttt atcaggacaa tacttaaatt ctgctagaaa
      301 aatccctatt aatctttggg ttaatggcaa acataaaaca atttctactg acaaaatagc
      361 aactaataaa aaactagtaa cagctcaaga aattgatgtt aaattaagaa gatatcttca
      421 agaagaatac aatatatatg gtcataataa cactggtaaa ggcaaagaat atggatataa
      481 atctaaattt tattcaggtt ttaataatgg gaaagtttta tttcatttaa ataatgaaaa
      541 a
//
LOCUS       DANBWH010000421          539 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.433, whole genome shotgun sequence.
ACCESSION   DANBWH010000421 DANBWH010000000
VERSION     DANBWH010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.433"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            91..>539
                     /locus_tag="PPY92_002869"
     CDS             91..>539
                     /locus_tag="PPY92_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDJ2626776.1"
                     /translation="MRDSKRVVLYISIMVLSIFIIGCGKGNEIKEDAKEEQIKKSFAK
                     TLDMYPIKNLEDLYDKEGYRDGEFKKGDKGMWTIYTDFAKSNRPGVLDNEGMVLNLDR
                     NTRTAKGYYFVDTIYDNHENSYSKNYRVEMKNNKIILLDKVEDQKLK"
BASE COUNT          234 a           53 c          111 g          141 t
ORIGIN      
        1 gatggattaa acttaattcc aagtgactaa aaataacaca aaggttgata gtactcaaca
       61 aatattaaca tgatagagag aagtgaaaaa atgagggatt caaaaagagt agtattgtac
      121 ataagcatta tggtgttgag tatttttata attggttgtg gcaaaggtaa tgaaataaaa
      181 gaagacgcaa aggaagaaca aatcaaaaag agctttgcga aaacgttaga tatgtatcca
      241 atcaaaaatc tcgaggacct atatgataaa gaaggatatc gagatggcga atttaaaaaa
      301 ggtgacaaag ggatgtggac gatttacaca gatttcgcca aaagtaatag accaggtgta
      361 ttagataatg aaggtatggt tttaaatttg gatagaaata cacgaacggc caagggatat
      421 tattttgtag atactatata tgacaatcat gaaaactctt atagtaaaaa ttatagagtt
      481 gagatgaaaa acaataaaat tattttatta gacaaggtgg aagatcaaaa acttaaaga
//
LOCUS       DANBWH010000422          533 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.434, whole genome shotgun sequence.
ACCESSION   DANBWH010000422 DANBWH010000000
VERSION     DANBWH010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.434"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
BASE COUNT          221 a           84 c           60 g          168 t
ORIGIN      
        1 ttgatgagaa ttttgagatg tagataaata tcaaactagt ctataggtaa aaatttataa
       61 ttaatttgaa aagaatgtct caaataatgt ttgaattagc aggtgtcata ctaagtaatg
      121 ttaagtaaac attttatact ttatcagaac aaaaaagaca tgcatcaaca tgtcagtgaa
      181 actaatccat attatcttaa caacgaaatt gaatcgaacc tgaaaaacgt atttacaaat
      241 cgcaactact ctatccaagg cgaaatcgca aaatctgatc cccgaaataa catatctcga
      301 aaaaaattcc ataattcaat catcttcaat aattaacaat acattactag ttccaaatat
      361 actcatacat acatgtttca ttgattgact gcatattgtc ataaataata ggggaactaa
      421 tcgctaataa tattgttaaa cacaatacat aataaagtga aagattttaa atacatgaat
      481 tgttcaccat tccgccatat attttcaatt acctaatttc cgaataaata tta
//
LOCUS       DANBWH010000423          532 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.435, whole genome shotgun sequence.
ACCESSION   DANBWH010000423 DANBWH010000000
VERSION     DANBWH010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.435"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..>532
                     /locus_tag="PPY92_002870"
     CDS             <1..>532
                     /locus_tag="PPY92_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1829 domain-containing protein"
                     /protein_id="HDJ2626777.1"
                     /translation="FGLENNGVSINKFKKRKKIFEEHLSAYGIKYNDKTHEIFVQTNF
                     KNFNKSKHNLLQCLIFVNDMYLLSNPKSQNIFTEDVANKLDEHNIYYGRDIPIIGSSG
                     VVHNFDFFISAKKNQKEKFINVFSNPNNSMIIKSKITDAMQAKKIKRHRQNEFIFILN
                     DSKKEINEHNKNLLHEN"
BASE COUNT          224 a           65 c           67 g          176 t
ORIGIN      
        1 tttggtttag aaaataacgg tgtttctata aataaattta aaaaacgtaa aaagattttt
       61 gaagagcacc tttcagctta cggtattaaa tataacgata aaactcacga aatttttgtt
      121 caaactaact ttaaaaattt taataaatcg aaacataatt tattacagtg ccttatattt
      181 gttaatgata tgtacttact ttctaatcct aagtcacaga acatatttac agaagatgtt
      241 gcaaacaaat tggatgaaca taacatttat tacggaagag atatacctat tataggaagc
      301 agtggtgttg ttcataattt cgactttttt attagcgcta agaaaaatca aaaagaaaaa
      361 tttatcaatg ttttttctaa ccctaataat tctatgatta ttaagtcgaa aataacggat
      421 gctatgcaag caaaaaaaat aaaaagacac aggcaaaatg agtttatttt tattttaaat
      481 gactcaaaaa aagaaataaa tgaacataat aaaaatcttc ttcatgaaaa ct
//
LOCUS       DANBWH010000424          518 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.436, whole genome shotgun sequence.
ACCESSION   DANBWH010000424 DANBWH010000000
VERSION     DANBWH010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.436"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(24..>518)
                     /locus_tag="PPY92_002871"
     rRNA            complement(24..>518)
                     /locus_tag="PPY92_002871"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          123 a          143 c          118 g          134 t
ORIGIN      
        1 agatgttccg aatatatcct tagaaaggag gtgatccagc cgcaccttcc gatacggcta
       61 ccttgttacg acttcacccc aatcatttgt cccaccttcg acggctagct cctaaaaggt
      121 tactccaccg gcttcgggtg ttacaaactc tcgtggtgtg acgggcggtg tgtacaagac
      181 ccgggaacgt attcaccgta gcatgctgat ctacgattac tagcgattcc agcttcatgt
      241 agtcgagttg cagactacaa tccgaactga gaacaacttt atgggatttg cttgacctcg
      301 cggtttcgct gccctttgta ttgtccattg tagcacgtgt gtagcccaaa tcataagggg
      361 catgatgatt tgacgtcatc cccaccttcc tccggtttgt caccggcagt caacttagag
      421 tgcccaactt aatgatggca actaagctta agggttgcgc tcgttgcggg acttaaccca
      481 acatctcacg acacgagctg acgacaacca tgcaccac
//
LOCUS       DANBWH010000425          505 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.437, whole genome shotgun sequence.
ACCESSION   DANBWH010000425 DANBWH010000000
VERSION     DANBWH010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.437"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..292
                     /locus_tag="PPY92_002872"
     CDS             <1..292
                     /locus_tag="PPY92_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HDJ2626778.1"
                     /translation="KYYEPLIHKKDKEFKEGMQRARKELNYTANTDVVSTLFSTKKNF
                     TKDNTVDDVIELSNKLKDKPNMPQKSQVTIQLGKPSINTKKPFYDDINPIEG"
BASE COUNT          227 a           57 c           81 g          140 t
ORIGIN      
        1 taaatattac gaaccattga ttcataaaaa ggataaagaa tttaaagaag gtatgcaacg
       61 agctagaaaa gagctaaact atactgctaa tactgatgtt gtttctacac ttttttctac
      121 taagaaaaac tttactaaag acaatacagt agatgatgtg attgaattga gtaataaatt
      181 aaaagataaa cccaatatgc ctcaaaaatc tcaagttact attcaattag gaaaaccaag
      241 tataaatact aaaaagccgt tttatgacga tataaatcca attgaaggat gagtgaatat
      301 aaagtgatta aaataaatga tagagacata gcagaattga gcagatattg agtttatcaa
      361 gatataaatg aaaaaagatt ttaaagtaaa tggaaaacgg ttcaaacaaa tagattattt
      421 taatgataaa aataacgata atatgaaaga tgcagttgat ataaaaacct acgagctact
      481 taatgatcag ggagatccaa ctggt
//
LOCUS       DANBWH010000426          479 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.438, whole genome shotgun sequence.
ACCESSION   DANBWH010000426 DANBWH010000000
VERSION     DANBWH010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.438"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            40..405
                     /locus_tag="PPY92_002873"
     CDS             40..405
                     /locus_tag="PPY92_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HDJ2626779.1"
                     /translation="MKKKKNAIKVIIILFISLIVAVAFFFGLKTYQGHKNIQLIDSYL
                     EEKNLKDKIKSEKTEYSAKKGLFYKEVTFKDEPGVTYVVQPISTNKGLFVEGFDTETK
                     KSLKTAKHKYFNQNYKPSK"
BASE COUNT          197 a           55 c           80 g          147 t
ORIGIN      
        1 tcgttcatac tgaatagata agcaaaaagg agttacataa tgaagaagaa aaaaaacgcg
       61 attaaagtta tcattatatt atttatttca ttaattgttg cagtagcatt tttctttggt
      121 ctgaaaacat atcaaggcca taagaatatt caactaattg acagttactt ggaagagaaa
      181 aatctaaaag ataaaattaa atcagaaaaa acagagtata gtgctaaaaa aggtctcttc
      241 tataaggaag ttacgtttaa agatgaacct ggtgtaactt atgttgtgca accaatcagt
      301 actaataaag gattatttgt tgaaggcttt gatacagaga ctaagaaaag tttgaaaact
      361 gcaaagcata aatatttcaa tcaaaattat aaaccatcaa aatagtgaaa cgttaaactg
      421 gtgaaacagt ttagtgcctt caattttaat tatttgaggt aaacagtttt tgtatggaa
//
LOCUS       DANBWH010000427          478 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.439, whole genome shotgun sequence.
ACCESSION   DANBWH010000427 DANBWH010000000
VERSION     DANBWH010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.439"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(135..335)
                     /gene="cspA"
                     /locus_tag="PPY92_002874"
     CDS             complement(135..335)
                     /gene="cspA"
                     /locus_tag="PPY92_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspA"
                     /protein_id="HDJ2626780.1"
                     /translation="MKQGTVKWFNAEKGFGFIEVEGENDVFVHFSAINQDGYKSLEEG
                     QAVEFEVVEGDRGPQAANVVKL"
BASE COUNT          160 a           89 c           65 g          164 t
ORIGIN      
        1 aatgttggtg atataaaaaa agagttctca atttggctga gaaactcatc gcgttaagta
       61 tttgtaataa gcatataaaa acaccttact tcttggtaag gtgttagtta aaatcaatga
      121 ttcaaatcta agaattatag tttaacaacg tttgcagctt gtggaccgcg gtcgccttca
      181 actacttcaa actcaacagc ttgaccttct tctaatgatt tgtaaccatc ttggttaatt
      241 gctgaaaaat gtacgaatac gtcattttct ccttcaactt cgataaagcc gaatcctttt
      301 tcagcgttaa accatttaac tgtaccttgt ttcataatct gaaacctcca agactaaaat
      361 tcattcaata tgcttattcg catattacaa aaaatacatg caaatttcac tcaaatattc
      421 tttgcaatat ggaataatca tctgcttaac ttgtattata gtgcagttat taccaaat
//
LOCUS       DANBWH010000428          467 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.441, whole genome shotgun sequence.
ACCESSION   DANBWH010000428 DANBWH010000000
VERSION     DANBWH010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.441"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..>467
                     /gene="fmhC"
                     /locus_tag="PPY92_002875"
     CDS             <1..>467
                     /gene="fmhC"
                     /locus_tag="PPY92_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhC"
                     /protein_id="HDJ2626781.1"
                     /translation="KNRGVFILVDPYLIENLRDANGRIIKNYNNSVIVKMLGKIGYLH
                     QGYTTGYSNKSQIRWISVLDLKDKDENQLLKEMEYQTRRNIKKTIEIGVKVEDLSIEE
                     TNRFYKLFQMAEEKHGFHFMNEDYFKRMQEIYKDKAMLKIACINLNEYQDKLK"
BASE COUNT          205 a           35 c           83 g          144 t
ORIGIN      
        1 aaaaatagag gagtatttat tcttgttgat ccatatttaa tagagaattt aagagatgca
       61 aatggtagga taataaagaa ttataataat tcagtgatag taaagatgct agggaaaatt
      121 gggtatctcc atcaaggtta tacaacagga tattcaaata aaagtcaaat taggtggatt
      181 tctgtattgg atttaaaaga taaagatgag aatcaacttt taaaagaaat ggaataccaa
      241 actagaagaa atataaaaaa gactattgag attggtgtta aggttgaaga tttatctatt
      301 gaagaaacaa atcgatttta taaattgttt caaatggctg aagaaaaaca tggttttcat
      361 ttcatgaatg aagattattt taaacgaatg caagaaatat ataaagataa ggcaatgtta
      421 aagatagctt gtataaatct taatgaatat caagataaat taaaaat
//
LOCUS       DANBWH010000429          446 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.442, whole genome shotgun sequence.
ACCESSION   DANBWH010000429 DANBWH010000000
VERSION     DANBWH010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.442"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..429)
                     /gene="sarU"
                     /locus_tag="PPY92_002876"
     CDS             complement(<1..429)
                     /gene="sarU"
                     /locus_tag="PPY92_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarU"
                     /protein_id="HDJ2626782.1"
                     /translation="MDYQTFEKVNKFINVEAYIFFLTQELKQQYKLSLKELLILAYFY
                     YKNEHSISLKEIIGDILYKQSDVVKNIKSLSKKGFINKSRNEADERRIFVSVTPIQRK
                     KIACVINELDKIIKGFNKERDYIKYQWAPKYSKEFFILFMN"
BASE COUNT          140 a           66 c           53 g          187 t
ORIGIN      
        1 gttcataaaa agtataaaaa attctttgct atattttgga gcccattgat attttatgta
       61 gtctctttcc ttattaaatc ctttaattat tttatctaac tcattaataa cacaagcaat
      121 ctttttacgt tgtattggag taactgaaac aaaaatacgg cgttcatctg cttcgtttct
      181 agacttattt ataaatcctt ttttagatag tgacttaatg ttctttacaa catcagattg
      241 tttgtaaagt atgtcaccaa tgatttcttt tagtgaaata ctgtgttcat ttttgtaata
      301 aaaatatgct aatatcaaca attcttttaa tgataattta tattgttgct ttaactcttg
      361 agtgagaaaa aatatgtacg cttctacatt tatgaattta ttgacctttt cgaaagtttg
      421 atagtccacc ttacattctc ctacta
//
LOCUS       DANBWH010000430          439 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.443, whole genome shotgun sequence.
ACCESSION   DANBWH010000430 DANBWH010000000
VERSION     DANBWH010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.443"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            63..269
                     /locus_tag="PPY92_002877"
     CDS             63..269
                     /locus_tag="PPY92_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626783.1"
                     /translation="MNEIETIISEIEKLLTNNTPYSISKNSGVPRQTVTDLKVGKTKI
                     KEAKFKTIIKLYEYQRTLENKTEC"
BASE COUNT          190 a           48 c           63 g          138 t
ORIGIN      
        1 ctaaacgtag ttactgcatt aggattatta taattataaa tagaaagaga gcgtgattaa
       61 aaatgaatga gattgaaact attataagtg aaatagaaaa gttattaact aacaatacac
      121 catatagtat ttcaaaaaat tcaggtgtac cacgtcaaac agttacagat ttaaaggtag
      181 gtaaaactaa aataaaagaa gctaaattta aaacgataat caagttatat gaatatcaaa
      241 gaacattaga aaataaaaca gaatgttaac aaaaatattt tgttaggtta ggtggacaag
      301 aaattaaatt tctaatgatt tcatggttgt ctgtccacgt tttgtccacc tcaataagat
      361 aatctcttag ttttagaaat tgtacatggt taaataatca aaaatgcata ttatgaaatt
      421 atcattgcca ttaaatctt
//
LOCUS       DANBWH010000431          438 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.444, whole genome shotgun sequence.
ACCESSION   DANBWH010000431 DANBWH010000000
VERSION     DANBWH010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.444"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..207)
                     /locus_tag="PPY92_002878"
     CDS             complement(<1..207)
                     /locus_tag="PPY92_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626784.1"
                     /translation="MKIKVKKEMRLDELIKWARENPDLSQGKIFFSTGFSDGFVRFHP
                     NTNKCSTSSFIPIDIPFIVDIEKEV"
     gene            complement(210..416)
                     /locus_tag="PPY92_002879"
     CDS             complement(210..416)
                     /locus_tag="PPY92_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626785.1"
                     /translation="MKIKIEKEMNLPELIQWAWDNPKLSGNKRFYSNDVERNCFVTFH
                     VDSILCNVTGYVSINDKFTVQEEI"
BASE COUNT          137 a           79 c           55 g          167 t
ORIGIN      
        1 tacttctttt tcaatatcaa ctatgaaggg gatatcaatt ggaataaaac ttgacgtcga
       61 acacttattt gtatttggat gaaaacgaac gaatccatca ctaaatcctg ttgaaaaaaa
      121 tatttttcct tgtgatagat ccggattttc tcgcgcccat ttaattaatt catctaatct
      181 catttctttt ttaactttga ttttcattgt tatatctcct cttgaacagt aaatttatcg
      241 ttaattgata catatccagt cacattacat aagatgctat caacatgaaa agtcacaaaa
      301 cagttgcgct caacatcatt tgaatagaat cttttattac ctgataactt ggggttatcc
      361 caagcccatt ggataagttc aggtaaattc atttcttttt caattttgat tttcattgtt
      421 tccgcccttt taaaataa
//
LOCUS       DANBWH010000432          436 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.445, whole genome shotgun sequence.
ACCESSION   DANBWH010000432 DANBWH010000000
VERSION     DANBWH010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.445"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..>436)
                     /locus_tag="PPY92_002880"
     CDS             complement(<1..>436)
                     /locus_tag="PPY92_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626786.1"
                     /translation="RSRLNVTTKILFLIKLIFSSLLLFISTIMLADYFSDYIDFIEYY
                     FYASMFIYIVFIPLIPRTKLLINPMDKQLLYRSSLREVDIFNLIYASDVLKKFVGYFN
                     LLIVGMAISLYHISPFIFNLKVFCALIYFSTISYVIQIIFINI"
BASE COUNT          196 a           55 c           48 g          137 t
ORIGIN      
        1 taatatttat aaaaataatt tgaattacat aagagattgt tgaaaagtat attagtgcac
       61 aaaaaacctt gaggttaaat ataaaaggac taatatgata taaactaata gccataccaa
      121 ctatcaacaa attaaaatat ccaacaaact tttttaaaac gtctgaagca tatatcaagt
      181 taaaaatgtc aacttctctc aaactagatc tatataacag ctgtttatcc attggattga
      241 tcaataattt agttctaggt attaatggta taaatacaat gtaaataaac atactcgcat
      301 agaaataata ttctataaaa tcaatataat cactgaaata gtcagctaac ataatagttg
      361 aaataaataa taatagtgac gaaaaaatca atttaattaa aaacagtatt tttgtagtaa
      421 cattaagtcg cgacct
//
LOCUS       DANBWH010000433          424 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.446, whole genome shotgun sequence.
ACCESSION   DANBWH010000433 DANBWH010000000
VERSION     DANBWH010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.446"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..>424
                     /locus_tag="PPY92_002881"
     CDS             <1..>424
                     /locus_tag="PPY92_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HDJ2626787.1"
                     /translation="KKEKRLNLILTVIQQNQFNKKQQIVDYMARHFGVYYSLTTISRD
                     LQELEIYKIPVENKKYIYKKINQTNQLSAKKQLEIFSDEIIEFITLNNYVLIKTSPGF
                     AQSISYYIDQLQMKEILGTIGGNDTLMILTSSNEIAEFV"
BASE COUNT          184 a           51 c           57 g          132 t
ORIGIN      
        1 tgaaaaaaga aaaacgttta aatcttatct taactgttat acaacagaat cagtttaata
       61 aaaaacaaca aatagtggat tacatggcaa gacattttgg agtttactat agcttgacaa
      121 ctatttctcg tgacttacaa gaattagaaa tttacaaaat ccctgttgaa aataaaaagt
      181 atatttacaa gaaaataaat caaacaaatc aattaagtgc aaaaaaacaa ttagaaatat
      241 ttagtgatga gattattgaa tttataacgc taaataacta tgtcttaata aaaacatctc
      301 ctggctttgc tcaaagtata agttattaca tagatcaatt acaaatgaaa gaaatattag
      361 gaactattgg aggtaacgat actttgatga ttttgacttc ttcaaatgaa atagcagaat
      421 ttgt
//
LOCUS       DANBWH010000434          408 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.448, whole genome shotgun sequence.
ACCESSION   DANBWH010000434 DANBWH010000000
VERSION     DANBWH010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.448"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            6..326
                     /locus_tag="PPY92_002882"
     CDS             6..326
                     /locus_tag="PPY92_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDJ2626788.1"
                     /translation="MQAIKSNESFKSIINSDTPVIVKFEAGWCPDCRAMDLWIDPIVE
                     QYNDYQWYTVNRDELEDVVVENEVMGIPSLLVFKNGDKIAHLHSANAKSPEQVESFLA
                     ETFK"
BASE COUNT          152 a           60 c           71 g          125 t
ORIGIN      
        1 ttacaatgca agcaatcaaa agtaatgaat catttaaatc tataattaat agcgatacac
       61 ctgtaattgt taaatttgag gcaggatggt gcccagactg tcgtgctatg gatttatgga
      121 ttgacccaat cgtagaacaa tataatgatt accaatggta tactgttaat cgtgatgaat
      181 tagaagatgt agttgttgaa aatgaagtta tgggtatccc tagcttgcta gtatttaaaa
      241 acggcgacaa aattgcacac cttcattcag caaatgctaa gtcacctgaa caagttgaat
      301 catttttagc agaaactttt aaataaatac taaaatacaa atgtgtatat acttcacaat
      361 aaaaagctga gactgtgatt gtaccgactc tagtaaatta gattaaat
//
LOCUS       DANBWH010000435          406 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.449, whole genome shotgun sequence.
ACCESSION   DANBWH010000435 DANBWH010000000
VERSION     DANBWH010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.449"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..>406
                     /locus_tag="PPY92_002883"
     CDS             <1..>406
                     /locus_tag="PPY92_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDJ2626789.1"
                     /translation="RLDGIPESMLIPLIARAKEYEYEKPIIKDALSKKIFDGLDDIYK
                     NVTCDDMSQIGISIRSVIIDSVTKRLIKDNKNLIVINIGCGLDTRFQRFNNEKISWID
                     LDVPESIEIRKTFFKETDSYKMIAKSMLDYSWI"
BASE COUNT          165 a           45 c           74 g          122 t
ORIGIN      
        1 cgactagatg gaattcccga atcaatgtta attcctttga tagctcgagc aaaagagtac
       61 gaatacgaaa aaccaataat aaaagacgca ctatctaaaa aaatatttga tggtttagat
      121 gatatataca aaaatgttac atgtgatgac atgtctcaaa ttggaattag tatacgttct
      181 gtgataatag atagtgttac taaaaggctt atcaaggata ataaaaattt gattgtgatc
      241 aatataggtt gtggcttaga tacaaggttt caaagattta ataacgaaaa aatatcgtgg
      301 atagatttag atgtacctga atcaatagaa atacgaaaaa cattttttaa agaaacagat
      361 agttataaga tgatagctaa atcaatgctg gattacagtt ggattg
//
LOCUS       DANBWH010000436          399 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.450, whole genome shotgun sequence.
ACCESSION   DANBWH010000436 DANBWH010000000
VERSION     DANBWH010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.450"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            12..>399
                     /locus_tag="PPY92_002884"
     CDS             12..>399
                     /locus_tag="PPY92_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDJ2626790.1"
                     /translation="MRYLKRLSWYISILILIVVIAGCGKGNETKEGSKEEQIKKSFAK
                     TLDMYPIKNLEDLYDKEGYRDSEFKKSDKGTWTIYTDFAKSNKPGELDDEGMVLNLDR
                     NTRTAKGHYFVTTFYRNGKLPDEKNYK"
BASE COUNT          164 a           48 c           83 g          104 t
ORIGIN      
        1 gacgtgaaac gatgcgatat ttaaaaagac tttcatggta cataagcatc ttaattttaa
       61 tagttgttat agctggttgt ggcaaaggta atgaaacaaa agaaggttca aaagaagaac
      121 aaatcaaaaa gagctttgcg aaaacgttag atatgtatcc aatcaaaaat ctcgaggatt
      181 tatacgacaa agaaggatat cgagatagcg aatttaaaaa aagtgataaa gggacatgga
      241 cgatatacac agatttcgct aaaagtaata aaccgggtga attggatgat gaaggtatgg
      301 ttttaaatct ggataggaat actagaaccg ctaaaggcca ttattttgtt actacatttt
      361 accggaatgg taaactacca gatgaaaaga attataaaa
//
LOCUS       DANBWH010000437          399 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.451, whole genome shotgun sequence.
ACCESSION   DANBWH010000437 DANBWH010000000
VERSION     DANBWH010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.451"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
BASE COUNT          137 a           59 c           63 g          140 t
ORIGIN      
        1 gtttctcacg gcggattatg ataatgatat ttaacacaat gatgaggtct ctattccaat
       61 aataaaatta gggtaatggc aacttctagt tgcacgtcat tgctcagaaa ataaatatca
      121 ttattcgtta ttcgttactt taacaattga tgttatggag gattggtggc atatcacaac
      181 gaagaaatat gttgacagta gcatatgttt gtacgtctaa taaagatttt attgtctacg
      241 tttatctttc atatccaacc actaagttac ttgaggttca tacattataa agaaaatgga
      301 cgaacagata aatcaatatg attatcattc gtccatatat cttttattaa tataatatta
      361 aacgcaaaaa aatgcctatc ctcttggata ggcatttgt
//
LOCUS       DANBWH010000438          388 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.452, whole genome shotgun sequence.
ACCESSION   DANBWH010000438 DANBWH010000000
VERSION     DANBWH010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.452"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..213
                     /locus_tag="PPY92_002885"
     CDS             <1..213
                     /locus_tag="PPY92_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDJ2626791.1"
                     /translation="VFSAFTFPLVISATALKNSLPVLMFPDIWKGLLFIEVLLATVIV
                     LRVFIGYLHFFLKKENQDKFLRNASQ"
BASE COUNT          122 a           68 c           55 g          143 t
ORIGIN      
        1 gtattttcag ctttcacatt ccctttagta atctcagcaa ctgctttaaa gaacagtttg
       61 cctgtactta tgtttccaga tatttggaaa ggtcttttgt ttatcgaagt gttattagcc
      121 actgtaatag tacttagagt ctttatagga tatcttcact tctttttaaa aaaggaaaat
      181 caagataaat ttcttcgtaa tgcgtctcag taacactatt accaagaatt aacacgcata
      241 tttaataact aaaaaagagg caggtaatct tgcctctttt tagtgtatat caatgcctct
      301 ttgatttaat atgatcttat caatgatacc ttctgactaa agctgtagta actatactag
      361 aacgtctagt tcataaacac gtttcact
//
LOCUS       DANBWH010000439          378 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.453, whole genome shotgun sequence.
ACCESSION   DANBWH010000439 DANBWH010000000
VERSION     DANBWH010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.453"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
BASE COUNT          157 a           50 c           50 g          121 t
ORIGIN      
        1 ttaaaatgtt ttataacaac atattttatg caacacataa aatgggtaac taacatcacg
       61 aaatcattgt atttgttgtt acactgatga agcgatgtca caaagaaatg tcgttatcaa
      121 acaaagcccg aaatacatac tgatactcga aataatgtaa cgataaataa caatgataac
      181 gattgattac tacgcaaaaa tatatgtaaa ttgacgattt aaatgcaatt cgtatatttt
      241 gccaatgatt tacacattaa aatgtttgaa aattgtcaca attcagtcta atttaaaagg
      301 aagttaatta caaaattcaa aaatcatgca tcgtgtcatt tagatattga aaaagatgag
      361 tcaaatttgt ttcatata
//
LOCUS       DANBWH010000440          351 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.455, whole genome shotgun sequence.
ACCESSION   DANBWH010000440 DANBWH010000000
VERSION     DANBWH010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.455"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..327
                     /locus_tag="PPY92_002886"
     CDS             <1..327
                     /locus_tag="PPY92_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626792.1"
                     /translation="WDKDKKVMSIIDEIDFNSGYILISTGYKSFNEVKLLQYTGFKDV
                     HGVEIYEGDIVQDCYSREVSFIEFKEGAFYITFSNVTELLSENDDIIEIVGNIFENEM
                     LLEVMR"
BASE COUNT          129 a           29 c           75 g          118 t
ORIGIN      
        1 tgggataaag ataaaaaagt tatgagtatt attgacgaaa tcgattttaa tagtgggtac
       61 attttgattt caacaggtta taaaagtttc aatgaagtaa aactattaca atacacagga
      121 tttaaagatg tgcacggtgt ggagatttat gaaggggata ttgttcaaga ttgttattcg
      181 agagaagtaa gttttatcga gtttaaagaa ggagcctttt atataacttt tagcaatgta
      241 actgaattac taagtgaaaa tgacgatatt attgaaattg ttggaaatat ttttgaaaat
      301 gagatgctat tggaggttat gagatgacgt tcaccttatc agatgaacaa t
//
LOCUS       DANBWH010000441          343 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.456, whole genome shotgun sequence.
ACCESSION   DANBWH010000441 DANBWH010000000
VERSION     DANBWH010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.456"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..>343
                     /locus_tag="PPY92_002887"
     CDS             <1..>343
                     /locus_tag="PPY92_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDJ2626793.1"
                     /translation="LMVNIINNWLVLFIPLLNWLTPKRYIKISQEELESLNTFKPAKN
                     NAFWPALGSSVLFSVTFRKYMPLFNVRLEKTIVIAIFFVVFLGILFFYLNLNRRLALS
                     VFTMNKEKSQKM"
BASE COUNT          123 a           37 c           54 g          129 t
ORIGIN      
        1 ttgatggtga atataataaa taattggtta gttttattca ttccattgct taattggttg
       61 acaccaaaaa gatacatcaa gatcagtcaa gaagaattag aaagtttaaa tactttcaaa
      121 cctgctaaaa acaatgcatt ttggccagca ttaggtagtt cagttttatt tagtgtgact
      181 tttagaaagt atatgccttt atttaatgtt agattggaaa aaacaatagt aatagccata
      241 ttttttgttg tatttcttgg tattttattt ttctacctaa atttaaatcg taggctagca
      301 ttaagtgttt ttactatgaa taaagaaaaa agtcagaaaa tga
//
LOCUS       DANBWH010000442          336 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.457, whole genome shotgun sequence.
ACCESSION   DANBWH010000442 DANBWH010000000
VERSION     DANBWH010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.457"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            78..>336
                     /locus_tag="PPY92_002888"
     CDS             78..>336
                     /locus_tag="PPY92_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDJ2626794.1"
                     /translation="MWIEKFKNKNNETKYRYYEKYKDPYTDKWKRVSVVLNKNTKQSQ
                     KEAMFRLEEKIKEKLNNKSSSELKTLTFHALLDEWLEYHIKT"
BASE COUNT          146 a           48 c           54 g           88 t
ORIGIN      
        1 taaattttgg gtagcccacc tacccttatt attttttata aatttacaga acgtacgttc
       61 ttgcaggagg tataaacatg tggattgaaa aatttaaaaa caaaaataac gaaactaaat
      121 acagatacta cgagaagtat aaagatccat acacagataa atggaagcgc gtaagtgttg
      181 tgttgaacaa gaatacaaaa caatctcaaa aagaagcaat gtttcgttta gaagaaaaaa
      241 taaaagaaaa actgaacaac aagtcgtcaa gcgaattaaa aactttgact tttcacgcgc
      301 tattagatga atggcttgaa tatcatataa aaacat
//
LOCUS       DANBWH010000443          319 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.462, whole genome shotgun sequence.
ACCESSION   DANBWH010000443 DANBWH010000000
VERSION     DANBWH010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.462"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            111..>319
                     /locus_tag="PPY92_002889"
     CDS             111..>319
                     /locus_tag="PPY92_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001656966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626795.1"
                     /translation="MSDNLSLFIDYINDNIIYGSEIKREKLENLFNQFAIKNVEKNIV
                     YDELKSLDITIIESQDSYKNKLKRL"
BASE COUNT          122 a           23 c           55 g          119 t
ORIGIN      
        1 tattgttttt taatttgtcg atatttaagt tgagttaaat ttaaagggtg taatttgttt
       61 tacaatgatg aagataatta gtctatcaaa ataaaggggt tgggactgtt atgagtgata
      121 atttgtcatt attcattgac tatatcaatg ataatataat ctatggtagt gaaatcaaac
      181 gggagaaatt agagaattta tttaatcaat ttgctataaa aaatgttgaa aagaacattg
      241 tctatgatga actgaaatct ttagatatta caatcattga gtcacaggat tcatataaaa
      301 ataaattgaa gagattatt
//
LOCUS       DANBWH010000444          315 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.463, whole genome shotgun sequence.
ACCESSION   DANBWH010000444 DANBWH010000000
VERSION     DANBWH010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.463"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(<1..213)
                     /locus_tag="PPY92_002890"
     CDS             complement(<1..213)
                     /locus_tag="PPY92_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HDJ2626796.1"
                     /translation="MDYIGQYAVIHLVLHVVCICIAYWALQSIRLDQFFKKGYATQLQ
                     VCMIFVAILLGTAVSNFIVDLLQYSTQ"
BASE COUNT          122 a           52 c           38 g          103 t
ORIGIN      
        1 ctgcgtcgag tattgtaaca aatctacaat aaaattgctt actgcagtgc ctaataaaat
       61 agcaacaaat atcatacaca cttgtaattg agtggcgtat ccttttttaa aaaattgatc
      121 taatctaatt gattgtaaag cccaataggc aatacaaata catacaacat gtaacactaa
      181 atggataact gcatattgtc cgatataatc cattttatca cctcataaga ttactgtatt
      241 acattgtaca tgaaaaattt aaataatgat acaaagttta atacccgttt ttagtaaagt
      301 ttaagctaac ttact
//
LOCUS       DANBWH010000445          308 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.465, whole genome shotgun sequence.
ACCESSION   DANBWH010000445 DANBWH010000000
VERSION     DANBWH010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.465"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            6..227
                     /locus_tag="PPY92_002891"
     CDS             6..227
                     /locus_tag="PPY92_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cyclic bacteriocin"
                     /protein_id="HDJ2626797.1"
                     /translation="MLKLHKKIAWTGIKGSAITTFITALYNGSSVWNALAVAGIAFGG
                     GAGTAVAALGRATVMRFIKRWGVRKTAAW"
BASE COUNT           92 a           46 c           72 g           98 t
ORIGIN      
        1 ggcctatgct aaaattacat aaaaaaatag cttggacagg aatcaaaggt tcagctataa
       61 ctacatttat tactgcctta tataatggaa gtagtgtttg gaacgcgcta gctgttgctg
      121 gtattgcttt tggtggtggc gctggtactg cggttgcagc tcttggtcgt gcaacagtaa
      181 tgagatttat caaacgttgg ggcgtacgaa aaacagctgc ttggtaaaag ataatctaat
      241 tctttataag agaggctttc ttttttggga aagcctcttt ttaaaaaagg agattcaatg
      301 aataattc
//
LOCUS       DANBWH010000446          291 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.470, whole genome shotgun sequence.
ACCESSION   DANBWH010000446 DANBWH010000000
VERSION     DANBWH010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.470"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..>291
                     /gene="ecb"
                     /locus_tag="PPY92_002892"
     CDS             <1..>291
                     /gene="ecb"
                     /locus_tag="PPY92_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="complement convertase inhibitor Ecb"
                     /protein_id="HDJ2626798.1"
                     /translation="KSILSIAAISLTVSTFAGESHAQTKNVEAAKKYDQYQTNFKKQV
                     NKKVVDAQKAINLFKRTRTVATHRKAQRAVNLIHFQHSYEKKKLQRQIDLVL"
BASE COUNT          127 a           48 c           42 g           74 t
ORIGIN      
        1 gaaatcaatt ttaagcatag ctgctattag tttaacggta tcaacgtttg ccggtgaatc
       61 tcatgcacaa actaaaaacg ttgaagctgc taaaaaatat gatcagtatc aaacaaactt
      121 taaaaaacaa gtaaataaaa aagttgtgga tgcacaaaaa gctataaact tgttcaaacg
      181 tacaagaact gttgcaacac accgtaaagc acaaagagct gttaatttaa ttcatttcca
      241 acacagctat gaaaagaaaa aattacaaag acaaatcgat ctagttttaa a
//
LOCUS       DANBWH010000447          279 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.denovo.471, whole genome shotgun sequence.
ACCESSION   DANBWH010000447 DANBWH010000000
VERSION     DANBWH010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.denovo.471"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            <1..>279
                     /locus_tag="PPY92_002893"
     rRNA            <1..>279
                     /locus_tag="PPY92_002893"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           89 a           56 c           76 g           58 t
ORIGIN      
        1 taagggcgca cggtggatgc cttggcacta gaagccgatg aaggacgtta ctaacgacga
       61 tatgctttgg ggagctgtaa gtaagctttg atccagagat ttccgaatgg ggaaacccag
      121 catgagttat gtcatgttat cgatatgtga atacatagca tatcagaagg cacacccgga
      181 gaactgaaac atcttagtac ccggaggaag agaaagaaaa ttcgattccc ttagtagcgg
      241 cgagcgaaac gggaagagcc caaaccaaca agcttgctt
//
LOCUS       DANBWH010000448        18118 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.guided.034, whole genome shotgun sequence.
ACCESSION   DANBWH010000448 DANBWH010000000
VERSION     DANBWH010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18118
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.guided.034"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            382..696
                     /locus_tag="PPY92_002894"
     CDS             382..696
                     /locus_tag="PPY92_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDJ2626799.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            712..1098
                     /locus_tag="PPY92_002895"
     CDS             712..1098
                     /locus_tag="PPY92_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDJ2626800.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            1127..2512
                     /locus_tag="PPY92_002896"
     CDS             1127..2512
                     /locus_tag="PPY92_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDJ2626801.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            2515..2667
                     /locus_tag="PPY92_002897"
     CDS             2515..2667
                     /locus_tag="PPY92_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDJ2626802.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            2690..3895
                     /locus_tag="PPY92_002898"
     CDS             2690..3895
                     /locus_tag="PPY92_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDJ2626803.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            3938..4159
                     /locus_tag="PPY92_002899"
     CDS             3938..4159
                     /locus_tag="PPY92_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626804.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            4276..4773
                     /locus_tag="PPY92_002900"
     CDS             4276..4773
                     /locus_tag="PPY92_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDJ2626805.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            4748..5254
                     /locus_tag="PPY92_002901"
     CDS             4748..5254
                     /locus_tag="PPY92_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDJ2626806.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            5238..7685
                     /locus_tag="PPY92_002902"
     CDS             5238..7685
                     /locus_tag="PPY92_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDJ2626807.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            7688..9865
                     /locus_tag="PPY92_002903"
     CDS             7688..9865
                     /locus_tag="PPY92_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDJ2626808.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGADRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRNNVNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            9862..10863
                     /locus_tag="PPY92_002904"
     CDS             9862..10863
                     /locus_tag="PPY92_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDJ2626809.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            10860..11792
                     /locus_tag="PPY92_002905"
     CDS             10860..11792
                     /locus_tag="PPY92_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDJ2626810.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            12037..12153
                     /locus_tag="PPY92_002906"
     CDS             12037..12153
                     /locus_tag="PPY92_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDJ2626811.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            12169..14088
                     /gene="tet(M)"
                     /locus_tag="PPY92_002907"
     CDS             12169..14088
                     /gene="tet(M)"
                     /locus_tag="PPY92_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HDJ2626812.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            14208..14375
                     /locus_tag="PPY92_002908"
     CDS             14208..14375
                     /locus_tag="PPY92_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDJ2626813.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(14435..14788)
                     /locus_tag="PPY92_002909"
     CDS             complement(14435..14788)
                     /locus_tag="PPY92_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDJ2626814.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            15293..15715
                     /locus_tag="PPY92_002910"
     CDS             15293..15715
                     /locus_tag="PPY92_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDJ2626815.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            15712..15942
                     /locus_tag="PPY92_002911"
     CDS             15712..15942
                     /locus_tag="PPY92_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDJ2626816.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            16403..16606
                     /locus_tag="PPY92_002912"
     CDS             16403..16606
                     /locus_tag="PPY92_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDJ2626817.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            16688..17905
                     /locus_tag="PPY92_002913"
     CDS             16688..17905
                     /locus_tag="PPY92_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDJ2626818.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
BASE COUNT         5944 a         3238 c         3765 g         5171 t
ORIGIN      
        1 tttggtcact ttaaattata gaggcaattg cgctttttgt atattaaaaa tagcataaaa
       61 atctagttat ccgcataaaa actggactta tcacacttta tcaaggtcaa aaccactcaa
      121 tttactacta atttactact tatgaatgag ctttgatacg acgatttatc cttgaaaagt
      181 gaagatataa agatacttcc aataaaattt gaatatttaa taggtagaca cttcaaaaaa
      241 tgaggtgtct atttttttac ccgattttga aaggaagtga acttatgaaa acaaaaaatc
      301 aagaatcaaa aggtcgttcc ccactcttta agaccatcaa acattcattc agccaataaa
      361 aaagaaagga taggtaaaaa tatggaactt aaatttgtga ttcccaacat ggaaaaaaca
      421 ttcggcaatt tagaatttgc tggcgaggat aaagtcgttc agcgaagaat caacggacgg
      481 ctaactgtct tatcaagaag ctataatctc tattctgatg ttcaaagagc agatgatatt
      541 gtggtggtgc ttcctgctga agctggcgaa aaacatttcg gctttgagga acgtgtgaag
      601 ttagtcaatc cacgtattac cgcagagggc tacaaaatcg gcactcgtgg ttttacaaat
      661 taccttttac atgctgacga catgataaaa gaataaagaa agagaggaaa aatgatgaga
      721 ttagcaaatg gcattgtatt agataaagac acgacttttg gagaattgaa attctctgct
      781 ctacgtcgtg aagtgagaat ccaaaatgaa gacgggtcgg tttcagatga aatcaaggaa
      841 cgtacctatg acttaaaatc caaaggacaa ggacgcatga ttcaagtaag tattcctgcc
      901 agcgtgcctt tgaaagagtt tgattataac gcacgggtgg aacttatcaa tcccattgcg
      961 gacaccgttg ctactgccac ctatcaagga gcagatgttg actggtatat caaggcagac
     1021 gatattgtgc tgacaaagga ttctagttca ttcaaagctc aaccacaagc aaagaaagaa
     1081 ccgacacaag acaaatagtc gctaggtaga aaggagactt tttcgcatga aacagcgtgg
     1141 taaaaggatt cgcccatctg gtaaagattt agtctttcat tttacgatag cgtcactcct
     1201 gcctgttttc ctgctggttg tcggactgtt tcatgtgaag acaatccagc agatcaactg
     1261 gcaggatttt aacctatcac aagcagataa gattgacatt ccctatttaa ttatcagttt
     1321 cagtgtcgca attcttatct gcttgctggt agcgtttgta ttcaaacggg ttcgctatga
     1381 tacggttaaa caactttacc accgtcaaaa actggcaaag atgatacttg aaaacaagtg
     1441 gtatgaatct gaacaggtca aaacagaggg tttctttaaa gatagtgctg gtcgtacaaa
     1501 ggaaaagata acctacttcc ctaaaatgta ttatcgactt aaaaatggct tgatacagat
     1561 acgggtggaa atcacgctgg gaaaatatca agaccaactc ttacacttgg aaaagaaatt
     1621 agagagtggc ttgtactgtg agctgacgga taaagagtta aaggattcct atgtggaata
     1681 tactttgctc tatgacacca tagccagtcg tatttctatt gatgaagtag aagctaaaga
     1741 tggtaaactt cgcttaatga aaaacgtatg gtgggaatat gataagctcc ctcatatgtt
     1801 gattgctggt ggtacaggtg gcggtaaaac ttactttata ctgacactga ttgaagcctt
     1861 gcttcataca gattcaaaac tgtatattct tgacccgaaa aatgctgatc ttgcggactt
     1921 aggttctgtg atggcaaatg tctactatag aaaagaagac ttgctttctt gcattgaaac
     1981 attctatgaa gaaatgatga aacgtagtga ggaaatgaag cagatgaaga actataagac
     2041 tggcaaaaat tatgcttact taggtctccc ggcacacttc ttaatctttg atgaatacgt
     2101 cgctttcatg gaaatgctgg gaacaaaaga aaacaccgca gttatgaata agctgaaaca
     2161 gattgtcatg ttaggtcgtc aagctggctt ctttctaata ctggcttgtc aacgtccaga
     2221 cgcaaaatat ttaggcgacg gaatccgtga tcagtttaat ttcagagtgg ctttaggtcg
     2281 tatgtctgaa atgggctatg gcatgatgtt tggcagtgac gtacaaaagg atttcttctt
     2341 aaagcgaatc aaaggtcgtg gctatgttga tgtaggaaca agtgtcatat cagagtttta
     2401 tactcccctt gtaccaaaag gatatgattt cttggaggaa attaaaaagt tatccaacag
     2461 cagacagtcc acgcaggcga cgtgcgaagc ggaagtcgca ggtgtggact gatcttgctg
     2521 gctggtgtgg caatagccac gccagcactt aaccccccgt atctaacagg ggggtacaaa
     2581 tcgacaggaa acagtcaaaa aaacattaga aaatcctttg gttacaaggg atttacaaaa
     2641 tttcagcgta tgtcaaatgg gctttaaaag ttgacatacg cctttttgat tggagggatt
     2701 tttactgaat gaacaaactt ggttacagca tttaaaagaa aaacgcttgg cttatggact
     2761 atctcaaaac cgtttagctg ttgcgactgg tattacaagg cagtatctaa gcgatattga
     2821 aacaggaaaa gtcaagccat cagaggattt acagcagtcc ctttgggaag ctctggaacg
     2881 cttcaatccc gacgctcccc ttgaaatgct gtttgattat gtaagaattc gctttccgac
     2941 aacagacgta cagcaggtgg tcgaaaacat cttacaactg aaactgtcct attttcttca
     3001 tgaggactat ggtttctatt cttattcaga gcattatgct ttaggcgaca tattcgtcct
     3061 ttgctcccat gaactggaca aaggagttct ggtggaattg aaaggtcgtg ggtgcagaca
     3121 atttgaaagc tatcttctgg cacaacaaag aagctggtat gagttcttta tggacgtttt
     3181 ggtggctggc ggtgtgatga aacgccttga ccttgccatt aacgataaga cagggatttt
     3241 aaatatccct gtactcactg aaaagtgcca acaggaagaa tgtatctccg tcttccgcag
     3301 ttttaaaagc tatcgcagtg gcgaactggt acgcaaagag gaaaaggaat gtatgggaaa
     3361 caccctctat atcggttcat tacaaagtga agtttatttc tgtatctatg aaaaggacta
     3421 cgagcagtac aagaaaaatg atattcccat tgaagacgca gaagtaaaaa accgttttga
     3481 gattcgattg aaaaatgagc gtgcctatta tgcagtccgt gatttactcg tctatgacaa
     3541 tccagagcat accgccttta aaattatcaa tcggtatatc cgttttgtag ataaagacga
     3601 ttccaaacct cgttctgatt ggaaactgaa tgaagaatgg gcttggttta ttgggaacaa
     3661 tcgtgaacga ttaaaactaa ccacaaaacc agagccttac tccttccaaa ggacgctgaa
     3721 ctggctatct catcaagttg ccccgacctt aaaggttgcg attaaacttg atgaaatcaa
     3781 ccagacgcag gttgtaaaag acattctcga ccatgcgaaa ctgacagacc gacacaagca
     3841 gattttgaag caacagtcag taaaagaaca ggacgtgata acaacaaaaa aataactcaa
     3901 atacaaattc attgaatata gagaggagaa catttttatg aattttggac aaaaccttta
     3961 taactggttt ctatcaaacg ctcaatcact ggtgctttta gcaatcgttg tgattggctt
     4021 gtatcttggc ttcaagcgtg agtttagcaa actgattggc tttttaatta ttgcgattat
     4081 tgcggttggc ttagtcttca acgctgctgg agtaaaagac attttactag agctattcaa
     4141 tcgcattatt ggtgcttaaa taaaaccgtt cttttgtgga atataagtgg ttttcttatg
     4201 ttccgcaaag gaatggtaca ccaaacgaag tgcggtaggg atttttgaat ctctacaaag
     4261 aaaggacgtg aatatatgga cgatatgcaa gtctatattg cgaatttagg caaatacaat
     4321 gagggcgaat tggtcggtgc gtggtttacc tttcccattg actttgagga agtcaaagag
     4381 aaaatcggct tgaatgatga atatgaggaa tacgccattc atgactacga gttacccttt
     4441 acggttgacg aatacacttc cattggcgaa ctcaatcgac tatgggaaat ggtatcggaa
     4501 ttacccgaag aattacaatc ggagctatct gctctgctca ctcatttttc aagcattgaa
     4561 gaactaagcg aacatcaaga ggatattatc attcattccg attgtgatga tatgtatgac
     4621 gtggcacgct actacattga agaaacgggt gctttaggcg aagtaccagc tagtcttcaa
     4681 aactatattg attatcaagc ctatggtcgg gatttagacc tttcaggaac gtttatctca
     4741 accaatcatg ggatttttga aatcgtctat taaatctgtc ggtacattac tactggcaga
     4801 ttttctattt tacggggtgg ctcaatcagc tacccctatt ttttatgaaa ggattgatta
     4861 catgaagaaa atacgaagct ataccagtat ctggtctgtg gaaaaggtac tgtattctat
     4921 caatgatttt agacttccgt ttcccataac ctttacgcaa atgacatggt ttgtcgtgtc
     4981 actctttgca gtgatgatac ttggcaactt gccccctctt tccatgatag agggagcatt
     5041 tctcaaatac tttgggattc ctgtggcttt cacatggttt atgtctacaa aaacttttga
     5101 tggtaaaaag ccttatggat ttttgaagtc tgtcattgct tatgcactgc gaccaaagct
     5161 gacctatgca ggaaaaaaag taacgcttgg cagaaaccag ccacaagaag ccattacagc
     5221 agttaggagt gaattttatg gcatatccaa ttaaatacat tgaaaacaat ctcgtctgga
     5281 ataaagacgg ggaatgttat gcttactatg agcttgttcc ttacaattac tcatttctaa
     5341 gtccagaaca gaaaatacaa gtgcatgatt ctttcagaca gcttatcgca caaaatcgtg
     5401 atggcaaaat tcatgcttta caaatcagta cagaatccag catacgttct gcacaagagc
     5461 gttccaaaaa tgaagtcact ggcaagctca aagcggttgc ctatgacaaa atcgaccaac
     5521 agacagacgc tttaatatcc atgattggcg aaaatcaagt gaactaccgt ttctttatcg
     5581 gctttaagtt gcttctcaac gatcaggagt tttctatgaa aagtcttacc gttgaagcaa
     5641 aaaatgcttt gtctgatttt gtctatgatg tgaaccataa gctgatgggc gattttgtta
     5701 gtatgagtaa tgatgaaatc ctgcgttttc agaagatgga aaagctctta gaaaataaaa
     5761 tctctcgtcg tttcaaaatc cgcaggttag ataaggacga cttcggctat ctgattgaac
     5821 acctttacgg acagacaggc actgcctatg aagagtatga gtaccatcta tcaaagaaaa
     5881 agctggataa tgaaacgctg attaaatact atgacttgat taagcctact cgctgtttgg
     5941 tggaagaaaa acagcgatat ttgaaaatcc agcaggaaga tgaaaccgtc tatgtagctt
     6001 actttaccat taacagcatt gtcggagaac tggacttccc gtcctctgaa atcttctact
     6061 accagcaaca gcaatttaca ttcccgattg atacgtcaat gaatgtggaa attgtagcga
     6121 atcgtaaagc cctatctact gtccgcaata aaaagaaaga actgaaagac ttggataacc
     6181 acgcttggca aagtgataat gaaaccagct ccaatgtggc ggaagctctg gaaagtgtga
     6241 atgagctgga aaccaattta gaccaaagca aggaatctat gtacaagctg tcttatgtgg
     6301 taagggtatc agcaaatgat cttgacgaac tcaaacgtcg ttgtaatgaa gtgaaagatt
     6361 tttatgacga tttaagcgta aaactggtac gaccatttgg ggatatgctc ggcttacatg
     6421 aagaattttt acctgccagc aagcgttata tgaatgatta tattcaatac gtgacctctg
     6481 atttcctcgc tggtttaggt tttggtgcta ctcaaatgct gggggaaaat gaggggattt
     6541 atgttggcta cagcttagat actggacgca atgtctatct gaaacctgct cttgccagtc
     6601 aaggggttaa gggttcagta accaatgcgt tagcgtcggc ttttgttggt tcgctgggtg
     6661 gtggtaaatc ctttgcgaat aaccttatcg tctattatgc ggtgctttat ggggcacaag
     6721 cagtgattgt agacccaaaa gcagaacgtg gcagatggaa agaaaccttg ccagagattt
     6781 cccatgaaat caatatcgtc actctgactt ctgatgagaa aaacaaaggc ttacttgacc
     6841 cttatgtgat tatgaaaaat cccaaagatt ctgaatcact ggctattgat attctgacat
     6901 tccttacggg gatttcctct cgtgatgggg aacgcttccc aatccttaga aaagccattc
     6961 gtgcagtaac caatagtgaa gtacgagggt tgatgaaagt gattgaggaa ttacgggttg
     7021 agaatacgcc actaagtacc agtatagccg accatatcga aagttttaca gactatgact
     7081 ttgcacattt attattcagt aatggttatg tggagcagtc tatcagctta gaaaaacaac
     7141 tgaacattat acaggttgcg gacttggtac ttcccgacaa ggaaacttcc tttgaggaat
     7201 ataccactat ggagctttta tccgttgcta tgctgattgt cattagtacc tttgctttag
     7261 actttatcca tacagaccga agcattttca agattgtaga tttagacgaa gcatggagct
     7321 ttttacaggt agcacaagga aaaacactat ctatgaagct ggttcgggct ggtcgtgcta
     7381 tgaacgctgg ggtatatttc gtgacccaaa atacagacga cctcttagat gaaaaactga
     7441 aaaataacct cggcttaaaa tttgcatttc gttccactga ccttaacgag attaaaaaga
     7501 ccttagcctt ttttggtgta gacccagagg acgaaaacaa tcagaagcga ttgcgtgatt
     7561 tggaaaacgg gcaatgcctt atcagtgatt tatatggtcg tgtcggtgtg atacagttcc
     7621 accctgtatt tgaagaactg ctccatgcct ttgataccag accacctgtg cgaaaagagg
     7681 tgtaaatgtg aaaccatcaa tagtaaacag aataaaatca aactggacgc tgaaacgtct
     7741 aggtaaagtg gcaatgacag tggctttcac acttgtgatt gccatttttc ttttagccat
     7801 gctgggaacg gtggttcaag ctgcgggctt ggtagatgat acggtcaatg tggcaaatga
     7861 atacagccga tacccacttg aaaactatca actggatttt tatgtggata atagctgggg
     7921 ctggcttccg tggaactggt cggacgggat tggaaaacag gtcatgtatg gactatatgc
     7981 cattaccaat tttatttgga caatcagttt gtatgtttcc aatgcgacag gttacttagt
     8041 acaggaagcc tattccttag acttcatttc cgctacagca gattccattg gtaagaatat
     8101 gcagacctta gctggtgtga gtgcaaacgg attttcaaca gagggtttct atgttggatt
     8161 cctcttactc ttgattttgg ttcttggggt ttatgttgcc tatacgggac tgataaagag
     8221 agaaaccaca aaggcaattc atgccattat gaattttgtg ctggtgttta tcctatcggc
     8281 ttcctttatt gcctacgctc ccgactacat taaaaaaatc aatgactttt catcagacat
     8341 cagtaatgcc agtttatcac ttggcacgaa gattgtcatg ccccattccg atagtcaagg
     8401 caaggacagc gtggacttaa tcagagatag cctgttttcc atacaggttc agcaaccgtg
     8461 gctactgctt caatacaaca gttcagacat tgaaagtatt ggtgctgacc gtgtggaaag
     8521 tctactttca accagtccag acagtaataa tggagaagac agagaaaaaa ttgtagcaga
     8581 agaaatcgaa gacagaaaca atgtgaacat gacgattaca aagaccatta accgtttagg
     8641 tacagtcttc ttcctatttg tcttcaatat tgggatttcc atatttgtat tcctattaac
     8701 aggaatcatg attttctcgc aggtactttt tatcatctat gctatgtttc tgcctgtgag
     8761 ctttatttta agcatgattc catcatttga tggtatgtca aaacgagcca taacaaagct
     8821 ctttaatacc attttgacac gagctggaat cacattgatt attacgacag catttagtat
     8881 ttcaaccatg ctctatacct tatcggctgg ttatccgttc tttttgattg cttttctaca
     8941 gattgtgacc tttgcaggaa tctacttcaa gctgggcgat ttaatgagta tgttttctct
     9001 acagagtaac gattctcaaa gtgtgggaag tcgtgtgatg agaaaacctc gtatgcttat
     9061 gcacgctcac atgcaccgtc tacagcggaa acttggacgt tccatgacta ctctaggggc
     9121 tgggtctgcc attgttacag gtaaaaaagg acagtcgggt tcggggagtt ctgcaaggac
     9181 acaagcagat cactcccgac cagacggaaa ggaaaaatca acacttggaa aacgtatcgg
     9241 tcaaaccatc ggtacagtag ctgataccaa agacagaatg gtagacactg ctagtggttt
     9301 gaaagaacag gttaaagatt tgccgaccaa tgcaagatat gcagtatatc aaggaaaatc
     9361 caaagtaaaa gagaatgtcc gtgatttaac cagtagtatt tctcaaacca aagcggacag
     9421 agccagtgga cgcaaggaac agcaggaaca aaggcgaaaa accattgcga agcgtcgctc
     9481 tgaaatggaa caggtcaaac agaaaaaaca gcctgcttct tctgttcatg aaagaccgac
     9541 tacaagacaa gaacaatatc atgatgaaca gacctcaaaa cagtctaata ttcagacttc
     9601 atataaggaa tctcaacaag ccaaacaaga gcgtccagca gttaagtccg atttttcaag
     9661 tccaaaagtg gaacgccaag gcaataccgt tcaagaaaaa accgttcaaa agccagcaac
     9721 ttcaaccact acagcagata gaacttcaca acgtccaatc acaaaagaac gtccgtctac
     9781 tgttcaaaga gtaccactac aaaatacaag aagtagacca ccaatcaaaa ccgccaccat
     9841 taagaaagtc ggtaagaaac catgaagttg aaaactttag tgattggtgg ttctggatta
     9901 ttcttgatgg tcttctcact gcttctgttt gttgccattt tattttcaga tgaacaggac
     9961 agcggaattt ccaatattca ttatggaggt gtgaatgttt ccgcagaagt gctggctcat
    10021 aagcctatgg tagaaaaata tgccaaagaa tatggcgttg aagaatatgt caacatactt
    10081 cttgcgatta tacaggtgga atcgggcggt actgcggaag atgttatgca gtcctcggaa
    10141 tccctcggtc ttccacctaa ttcattgagt acagaagaat ccattaagca aggtgtgaag
    10201 tatttcagtg aattattagc cagtagcgaa aggctcagtg tagatttaga atcggttatc
    10261 cagtcctaca attatggtgg tggtttctta gggtatgtgg ctaatcgtgg aaataaatat
    10321 acctttgaac tggctcaaag tttctcaaaa gagtattcag gtggcgaaaa agtgtcttac
    10381 cccaatccca tagccatacc tatcaatggg ggctggcgat acaactatgg caatatgttt
    10441 tatgtgcaac tggtaacgca gtatcttgtc acaacagagt ttgatgatga tacggtacaa
    10501 gccatcatgg acgaagcact gaaatatgag ggctggcgat acgtttacgg tggagcttcc
    10561 ccgactactt cttttgattg tagcggactg acacaatgga cgtatggaaa agctggaatt
    10621 aacttaccac gaaccgcaca acagcaatat gatgtgaccc agcatatccc actatcggaa
    10681 gcacaagctg gcgatttggt tttctttcat tctacctata acgctggctc ttatattact
    10741 catgttggga tataccttgg caataaccgt atgtttcatg caggcgaccc aatcggttat
    10801 gccgacttaa caagccccta ctggcaacag catttagtgg gagcaggacg aatcaaacaa
    10861 tgagaaagga agatttaatg atgaaattta gaaaaaatca gaataaagaa aaacagatac
    10921 caaaggaaaa gaaacctcgt gtctataagg tcaatcctca taaaaaggtt gtgattgcct
    10981 tgtgggtact tttagggctt agtttcagct ttgcgatatt caagcacttt acagctatag
    11041 atactcatac tattcacgaa acaactatca tagaaaagga atacgttgat actcatcatg
    11101 tagaaaattt tgtagagaac tttgcgaaag tctactattc atgggagcaa tccgataagt
    11161 ccattgataa tcgaatggaa agtctaaaag gctatctgac agatgaactt caagctctca
    11221 atgttgatac agtacgcaaa gatattcctg tatcgtcttc tgtaagagga tttcagatat
    11281 ggacggtaga gccaactggc gacaatgagt ttaatgtaac ctacagtgta gaccagctca
    11341 ttacagaggg agaaaataca aagaccgtcc actctgctta tatagtgagt gtctatgtag
    11401 atggttctgg aaatatggta ctggttaaga atccgaccat taccaacata cctaagaaat
    11461 caagttataa accaaaagcc attgaaagtg aggggacggt tgattccatt acaaccaatg
    11521 aaatcaatga gtttttaacg acgttcttca agctctatcc tacagcgaca gccagtgaac
    11581 tttcctacta tgtgaatgac gggatattaa aaccaatcgg aaaagagtac atctttcaag
    11641 aactggtaaa tcctattcac aatcgtaagg ataatcaagt cacggtatcg ctgacagtgg
    11701 agtatatcga ccagcagacc aaagcaacgc aggtatctca atttgatttg gtacttgaaa
    11761 agaacgggag taattggaag attatagaat aacaaatatt ggtacattat tacagctatt
    11821 ttgtaatcac gtactctctt tgataaaaaa ttggagattc ctttacaaat atgctcttac
    11881 gtgctattat ttaagtatct atttaaaagg agttaataaa tatgcggcaa ggtattctta
    11941 aataaactgt caatttgata gtgggaacaa ataattggat gtcctttttt aggagggctt
    12001 agttttttgt acccagttta agaatacctt tatcatgtga ttctaaagta tccggagaat
    12061 atctgtatgc tttgtatgcc tatggttatg cataaaaatc ccagtgataa aagtatttat
    12121 cactgggatt tttatgccct tttgggtttt tgaatggagg aaaatcacat gaaaattatt
    12181 aatattggag ttttagctca tgttgatgca ggaaaaacta ccttaacaga aagcttatta
    12241 tataacagtg gagcgattac agaattagga agcgtggaca aaggtacaac gaggacggat
    12301 aatacgcttt tagaacgtca gagaggaatt acaattcaga caggaataac ctcttttcag
    12361 tgggaaaata cgaaggtgaa catcatagac acgccaggac atatggattt cttagcagaa
    12421 gtatatcgtt cattatcagt tttagatggg gcaattctac tgatttctgc aaaagatggc
    12481 gtacaagcac aaactcgtat attatttcat gcacttagga aaatggggat tcccacaatc
    12541 ttttttatca ataagattga ccaaaatgga attgatttat caacggttta tcaggatatt
    12601 aaagagaaac tttctgccga aattgtaatc aaacagaagg tagaactgta tcctaatatg
    12661 tgtgtgacga actttaccga atctgaacaa tgggatacgg taatagaggg aaacgatgac
    12721 cttttagaga aatatatgtc cggtaaatca ttagaagcat tggaactcga acaagaggaa
    12781 agcataagat ttcagaattg ttctctgttc cctctttatc atggaagtgc aaaaagtaat
    12841 atagggattg ataaccttat agaagttatt actaataaat tttattcatc aacacatcga
    12901 ggtccgtctg aactttgcgg aaatgttttc aaaattgaat atacaaaaaa aagacaacgt
    12961 cttgcatata tacgccttta tagtggagta ctacatttac gagattcggt tagagtatca
    13021 gaaaaagaaa aaataaaagt tacagaaatg tatacttcaa taaatggtga attatgtaag
    13081 attgatagag cttattctgg agaaattgtt attttgcaaa atgagttttt gaagttaaat
    13141 agtgttcttg gagatacaaa actattgcca cagagaaaaa agattgaaaa tccgcaccct
    13201 ctactacaaa caactgttga accgagtaaa cctgaacaga gagaaatgtt gcttgatgcc
    13261 cttttggaaa tctcagatag tgatccgctt ctacgatatt acgtggattc tacgacacat
    13321 gaaattatac tttctttctt agggaaagta caaatggaag tgattagtgc actgttgcaa
    13381 gaaaagtatc atgtggagat agaactaaaa gagcctacag tcatttatat ggagagaccg
    13441 ttaaaaaatg cagaatatac cattcacatc gaagtgccgc caaatccttt ctgggcttcc
    13501 attggtttat ctgtatcacc gcttccgttg ggaagtggaa tgcagtatga gagctcggtt
    13561 tctcttggat acttaaatca atcgtttcaa aatgcagtta tggaggggat acgctatggc
    13621 tgtgaacaag gattgtatgg ttggaatgtg acggactgta aaatctgttt taagtatggc
    13681 ttatactata gccctgttag taccccagca gattttcgga tgcttgctcc tattgtattg
    13741 gaacaagtct taaaaaaagc tggaacagaa ttgttagagc catatcttag ttttaaaatt
    13801 tatgcgccac aggaatatct ttcacgagca tacaacgatg ctcctaaata ttgtgcgaac
    13861 atcgtagaca ctcaattgaa aaataatgag gtcattctta gtggagaaat ccctgctcgg
    13921 tgtattcaag aatatcgtag tgatttaact ttctttacaa atggacgtag tgtttgttta
    13981 acagagttaa aagggtacca tgttactacc ggtgaacctg tttgccagcc ccgtcgtcca
    14041 aatagtcgga tagataaagt acgatatatg ttcaataaaa taacttagtg tattttatgt
    14101 tgttatataa atatggtttt cttgttaaat aagatgaaat attttttaat aaagatttga
    14161 attaaagtgt aaaggaggag atagttatta taaactacaa gtggatattg tgtcctgtat
    14221 gtggaaataa aacacgatta aagataaggg aagatactga attaaaaaaa ttccccctct
    14281 attgtccgaa atgcagacaa gaaaatttaa ttgaaataaa gcagttcaaa gtaactgtga
    14341 ttacagagcc agacgcaaag acgcagagcc gataaaatga gattaataca atctcatttt
    14401 atcggctctt tccgttatgt atggattctt ttaattagtc ttcgatgttt cttgcttcgt
    14461 tgataccgct ggctaaagat tccattaagg atagttcttt gtctgtaaag ctatccatgt
    14521 atttctctat ctgtaatcgt cgggtgcttt ttaccaagtt attagcaggt aagaaaaatt
    14581 catcaacgga aacatgaagt aacgatacaa ggtcataaag aacttgtatg ctggggtgtt
    14641 gccctttatt ttcaatatta gttaagtacc gtgggtcaat ttcaatcaat gctcccactt
    14701 gttcacgagt taaacctcgt ttcaatcgag cttctttaat ggctaaacca aaggctctaa
    14761 aatcatattt atcttctttt ttacgcatag tagaccacct ctatacattt tattgttcct
    14821 actgaattaa aaacaggtat agaaaaacgt gttatatggt ttataggttt atatttaata
    14881 aaaagcacta ctaaacgcca ataaaaaaaa ccgttatatg gtagtgctat ttacgctgtt
    14941 aaaatattgt atattacttc caaatggcgg tttgttggag gtcaacgtcg ccatgaagta
    15001 catcatatac aataaatttc cttacattgg gttcttgtca aaaaaagtcg tctatctgca
    15061 atagataagt acgtccacca atgtggtttt ataaatcata tagatagaat aacagaagca
    15121 tgtaaacaga gaaataaatc tgtttatatg cttttttggc tattcagaac ttttttacaa
    15181 agtttattta tcagtaatgc aacaaatccc cctttcacat tgggactaag agtgaaagga
    15241 gataaacgag caaggctcac ttcctttcct agacagaaag ggggtgagaa acatgaaacc
    15301 atcttctttt cagaccacaa tagaaaatca gtttgactat atctgtaaac gtgctatgga
    15361 agacgagcga aagaattata tgctttatct ttcaaggatt gcaaagcgtg aggtgtcctt
    15421 ttcggatgtt ggcgattatc ttgttagcca gtttgcgaca acagataact attcaactga
    15481 ctttcagatt tttacactca atgggttatc agtaggcgtt gaaaatgatt tgttgagtga
    15541 agcattacgt gagttgccag acaagaaacg tgaaattcta ctgctgtttt actttatgga
    15601 catgagcgat tcagaaattg cagacctgtt gaaattgaac cgttctactg tctatcggca
    15661 tagaaccagt ggactagcct taattaaaaa gtttatggag gaatttgaag aatgaaaaca
    15721 caatatccta tgattccctt tcctctcatt gtaaaggcaa cagatggcga taccgaagcg
    15781 attaaccaga ttctacatca ttacagaggg tacataacga agcgttccct acgacttatg
    15841 aaagatgaat atggcaatca aagtatggtc gttgatgaag tcttacgtgg aagaatggaa
    15901 accagactga ttacaaagat tttgtcattt gaaattaagt aatatcctct ctcctttcgt
    15961 ggaagcgtgc taaaccattc cacgcttccc gaacagggag gtttgttatt ccaccaaagc
    16021 atattgagct ttcaatgtgt tttgataggc taacgagcca ttgttctttg aaaactgaat
    16081 aaaagtaatc gaatacgttt cgataagaaa agagccaacg gaactaaccg ccatgaccta
    16141 tcttataaag atagcgagcg attcatgtta gtgatccgag aagcaatctt tagcaggatt
    16201 gcctgcaacg acattcttat cgtgataatg atactcccat acagtcaata gtccgagcgt
    16261 gataaaaccg tcgcaggcaa tgagtatggc tacatgagaa ccatgcaggg gtggaactcc
    16321 cgtgagcttt gctaaagctg ttcgattgct ggtaaaacaa cttttatgaa atccaaataa
    16381 gtgatttgga aaggaggatt ttatgaagca gactgacatt cctatttggg aacgttatac
    16441 cctaaccatt gaagaagcgt caaaatattt tcgtattggc gaaaacaagc tacgacgctt
    16501 ggcagaggaa aataaaaatg caaattggct gattatgaat ggcaatcgta ttcagattaa
    16561 acgaaaacaa tttgaaaaaa ttatagatac attggacgca atctagcgtc gccaaagggt
    16621 cttgtatatg ataaaatagt attaagtcgt atcaaggctc tttccataaa ggaaaggagc
    16681 aaatgccatg tcagaaaaaa gacgtgacaa taaaggtcga atcttaaaga ctggagagag
    16741 ccaacgaaaa gacggaagat acttatacaa atatatagat tcatttggag aaccgcaatt
    16801 tgtttactcg tggaaacttg tggctacaga ccgagtacca gcaggaaagc gtgattgtat
    16861 ctcacttaga gagaaaatcg cagagttaca gaaagacatt catgatggta ttgatgttgt
    16921 aggaaagaaa atgacactct gccagcttta cgcaaaacag aacgctcaaa gaccaaaggt
    16981 tagaaaaaac actgaaactg gacgcaaata tcttatggat attttgaaga aagacaagtt
    17041 aggtgtaaga agtattgaca gtattaagcc atcagacgct aaagaatggg ctattagaat
    17101 gagtgaaaat ggttatgctt atcaaaccat caataactac aaacgttctt taaaggcttc
    17161 attctatatt gctatacaag atgattgtgt tcggaagaat ccatttgact ttcaactgaa
    17221 agcagttctt gatgatgata ctgtccctaa gaccgtacta acagaagaac aggaagaaaa
    17281 actgttagcc tttgcaaaag ctgataaaac ctacagcaaa aattatgatg aaattctgat
    17341 actcttaaaa acaggtcttc gtatttcaga gtttggtggt ttgacacttc cagatttaga
    17401 ttttgagaat cgtcttgtca atatagacca tcagctattg agagatactg aaattgggta
    17461 ctacattgaa acaccaaaga ccaaaagtgg cgaacgtcaa gttcctatgg ttgaagaagc
    17521 ctatcaagca tttaagcgag tgttagcgaa tcgaaagaat gataagcgtg ttgagattga
    17581 tggatatagt gatttcctct ttcttaatag aaagaactat ccaaaagtgg caagtgatta
    17641 caacggcatg atgaaaggtc ttgttaagaa atacaataag tataacgagg ataaattgcc
    17701 acacatcact ccacatagtt tgcgacatac attctgtacc aactatgcaa atgcaggaat
    17761 gaatccaaag gcattacagt acattatggg acatgctaat atagccatga cgctgaacta
    17821 ttacgcacat gcaacattcg attctgcaat ggcagaaatg aaacgcttga ataaagagaa
    17881 gcaacaggag cgtcttgttg cttagtagta caaatgaatt tactacttat ttaccacttc
    17941 tgacagctaa gacatgagga aatatgcaaa gaaacgtgaa gtatcttcct acagtaaaaa
    18001 tactcgaaag cacatagaat aaggctttac gagcatttaa gaaaatataa aaagataatt
    18061 agaaatttat actttgtttg tattaaaaaa agcaggaagt tttaccttcc caccataa
//
LOCUS       DANBWH010000449        16484 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.guided.039, whole genome shotgun sequence.
ACCESSION   DANBWH010000449 DANBWH010000000
VERSION     DANBWH010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16484
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.guided.039"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            159..515
                     /locus_tag="PPY92_002914"
     CDS             159..515
                     /locus_tag="PPY92_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626819.1"
                     /translation="MLKQILPRATKISILFAVAFFIINYIGMEKPDILYLVGRTIIAT
                     LAFILICLTLFTIINSPERKIKLGTTLPIALIIGIIFGAIFLTVQIGVITGLIIGVIA
                     TFIWELIEKNKGGRSS"
     gene            512..697
                     /locus_tag="PPY92_002915"
     CDS             512..697
                     /locus_tag="PPY92_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626820.1"
                     /translation="MTTALIGVIVLILLIISFVPNYRAMKSAKEQGSNATRATIMVGI
                     DIILIVLIIVTLLLKYM"
     gene            complement(793..984)
                     /locus_tag="PPY92_002916"
     CDS             complement(793..984)
                     /locus_tag="PPY92_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626821.1"
                     /translation="MMGYIILFFLAGPVILGVGNLVIGPIFNKQTPFRVQVRSFVVGS
                     MIYLILATIGYFLLLQGKL"
     gene            complement(995..1630)
                     /locus_tag="PPY92_002917"
     CDS             complement(995..1630)
                     /locus_tag="PPY92_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDJ2626822.1"
                     /translation="MYRAVIFDFDGTIIDTEQHLFNVINKHLEMHNADPISIDFYRSS
                     IGGAATDLHDHLIKAIGSENKDKLYEEHHLTSTTLPMIDTIKSLMAFLKQRHIPMAIA
                     TSSVKAEIMPTFKALGLDDYIEVVVGREDVEQVKPDPELYLSAVQQLNYMPTQCLAIE
                     DSVNGATAAIAAGLDVIVNTNKMTSAQDFSNVDYVAKDIDYDQIVARFFTK"
     gene            complement(1705..2622)
                     /locus_tag="PPY92_002918"
     CDS             complement(1705..2622)
                     /locus_tag="PPY92_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HDJ2626823.1"
                     /translation="MFQKLSIFATKSFLVWMLVAAVIGFIFPQHVATLGKWVPYLLGI
                     VMLGMGLTITPNDFKMVFKAPRAVIIGVCLQFSIMPTLAFIIAKSFHLPPDIAVGVIL
                     VGCCPGGTSSNVMSYLAKANVALSVSITTVSTLLAPFVTPALIYLFANEWLEVSFLSM
                     LWSVVQVVLIPIALGIVLQIINRKIAEKASTALPIISVVAISLILAIVVGGSKHQILT
                     TGLLIFLVVILHNVLGYTIGYWLARLLKLDRQDQKAVSIEVGMQNSGLAVSLAALHFN
                     PIAAVPGAVFSFIHNITGPILAKYWSKKL"
     gene            2890..3420
                     /locus_tag="PPY92_002919"
     CDS             2890..3420
                     /locus_tag="PPY92_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626824.1"
                     /translation="MLIDKFETYIINIAGLNDRTTRKKLSKLCKSVQFCDALQFSINK
                     QFNQYVLEISLPKQQLPYFISFLSFHQYSIFQVLSPKKINELLDSDNLYQSAKRFDIN
                     IDGLQDAFIKDKVIDIMNMFQNHTDITYTLNKSHAHIICTPEIFAKLLHTIATRNIDI
                     LSANYRSSSMSKARIS"
     gene            complement(3486..5090)
                     /locus_tag="PPY92_002920"
     CDS             complement(3486..5090)
                     /locus_tag="PPY92_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDJ2626825.1"
                     /translation="MNAIKRFGSAMIVPVLMFAFFGIVLGFATLFKNPTIMGSLADQH
                     TFWFKFWSVIESGGWVIFTHMEVVFVVGLPLSLAKKAPGHAALAALMGYLMFNTFINA
                     ILTQWPHTFGANLEKGVENVPGLKSIAGIATLDTNILGGIIISAIITWIHNRYYSKRL
                     PEMVGVFQGLTFVVTISFFVMLPLAAITCVIWPTVQHGIGSMQHFIIASGYIGVWLYH
                     FLERVLIPTGLHHFIYAPIEVGPVVVNHGLKAEWLQHLNEFAKSTKPLKEQFPYGFML
                     QGNGKVFGCLGIALAMYATTPKENRKKVAALLIPATLTAVVVGITEPLEFTFLFIAPY
                     LFVLHAVLAASMDTLMYAFGVVGNMGGGLLDFISTNWLPLGKEHWGTYVAQVIIGLIF
                     VAIYFFLFRFLILKFDIPLPGRKKTEEEVKLFSKQDYKNKKGDSVDSKRASTGNEYED
                     KAAYYLDGLGGKENIKDVTNCTTRLRLTVYDESKVADTEYFTHQQMAHGLVKSGKSIQ
                     VVVGMTVPQVREAFEQMVEDQSSEDK"
     gene            5420..6184
                     /locus_tag="PPY92_002921"
     CDS             5420..6184
                     /locus_tag="PPY92_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDJ2626826.1"
                     /translation="MFLDEHINRNFDKLNDNDLHIAHFINTHIDECKNMKIQDLAQFT
                     HASNATIHRFTRKLGFDGYSDFKSYLKFESNKTHQLPSNSIDSFKQEIDSTFSYLERV
                     DYQLLTEKMHTASTIYLYGTGRAQLNVASEAQRILLTLHNHVIVLHDEHELKMILNKS
                     EPNDLFFIISLSGETHELLEVTNLLQLRQKYFISVTTMKDNTLAQKANYNVYVSSNTF
                     YLTDGTDYSSFISYHIFFETLVRKYNEYLLHNDISN"
     gene            complement(6242..6748)
                     /locus_tag="PPY92_002922"
     CDS             complement(6242..6748)
                     /locus_tag="PPY92_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HDJ2626827.1"
                     /translation="MAKFNVENEHVEVEIEKLYKFSPELVYEAWTKKDLLKQWFMTSA
                     RTNKEIEADVKEGGKYRIVDQQRNGKVNVIEGIYESLVMDEYVKMTIGMPGLSETQDV
                     IEVEFFERETGGTQMLFYYRSLVEKERRFTNLEYKQKKKEYHDAMVHGFELMFDKMYH
                     VIETSTQQ"
     gene            complement(6915..8228)
                     /locus_tag="PPY92_002923"
     CDS             complement(6915..8228)
                     /locus_tag="PPY92_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HDJ2626828.1"
                     /translation="MEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITIT
                     VIVALLMNRKESFAKKVEVFTKGAGHSNIILMMLIFILAGAFSNTAEKMGGVKSTVNL
                     GLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVV
                     GGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISL
                     NHAKNYDYNLIKVVPYILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDA
                     VSKGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSV
                     ADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAA
                     GVAGISPLMLFPYSIYPILLGVCGLIAILFNFPKLKKNTSQEMKH"
     regulatory      complement(8305..8400)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(8453..9022)
                     /locus_tag="PPY92_002924"
     CDS             complement(8453..9022)
                     /locus_tag="PPY92_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDJ2626829.1"
                     /translation="MALIVSRAILFLILALAFGIATVFPEQKLPLYIVFTLFVAVAII
                     MNVWFTHQNVKKHIIKFNKYVAYFLSFVSGLLYIGFVIYTLTIGSYDVLPIIIFAISI
                     VLVQILENILGISIKNTRLSNKQLKVISKQRVQQFKVISLILVSVSIVFIFIALMLKW
                     WLLLVISIIVLIIITVAMIVYEKYYTTPD"
     gene            complement(9509..10390)
                     /locus_tag="PPY92_002925"
     CDS             complement(9509..10390)
                     /locus_tag="PPY92_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDJ2626830.1"
                     /translation="MATQDPRTKFKTTEYEKQEQEVPGLQSEMTPAPDCGETSYQGHQ
                     RLQGYKMLVTGGDSAIGRAAAIAYAKEGADVAINYLPSEEQDAQEVRQVIEESGQKAV
                     LIPGDIRDEQFNYDLVEQAYQQLGGLDNVTLVAGHQQYHDDIHGFTTEAFTETFETNV
                     YPLFWTVQKALEYLKPGASITTTSSVQGYNPSPILHDYAASKAAIISLTKSFSEELGP
                     KGIRVNCVAPGPFWSPLQISGGQPQSKIPTFGQKTPLGRAGQPVELCGTYVLLASEES
                     SYTTGQVFGVSGGVQID"
     gene            complement(10501..11622)
                     /locus_tag="PPY92_002926"
     CDS             complement(10501..11622)
                     /locus_tag="PPY92_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HDJ2626831.1"
                     /translation="MGLKDQAVTWRRYFHQFPELSDKEFKTTQKIKDILTEHHIRILD
                     LPLMTGLVAEVGQGLSCIAVRADIDALPIQELVEQDFKSENEGVMHACGHDIHMASIL
                     ATAVKLKEIEGTLTGRVKFIFQPAEELGHGAFKIIETDALKDVQAVLGFHNDPSRSVG
                     TFAIKTGAITSAVDRFEFHIKGVGGHAAKPEQCNDPVIVLAQLINSIQSIVSRNLSAF
                     DEAVVTIGQISCGNTWNVIADHAYVQGTVRSFDPVVRKLVETRLQDIADGLAQAYNMK
                     INLNYTHLPGAVMNDEALTHKAIAVAQHVGYKVEMMEQPLTIGEDFSGYSQHFPSVFA
                     LIGSHSEYDLHHPQYKPDERILEKVPEYFVEFVKRLLHE"
     gene            complement(11898..13136)
                     /locus_tag="PPY92_002927"
     CDS             complement(11898..13136)
                     /locus_tag="PPY92_002927"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HDJ2626832.1"
                     /translation="MNDLIINHIAELILPKSTDKPLKGKELDELNVVKNGTVVIKDGK
                     IVYAGQHTDDYDATETIDASGKVVSPALVDAHTHLTFGGSREHEMSLKRQGKSYLEIL
                     EMGGGILSTVNATRETSEDDLFKKAEHDLLTMIKHGVLAVESKSGYGLDRENELKQLK
                     VSNRLAEKYDLDMKHTFLGPHAVPKEASSNEAFLEEMIALLPEVKQYADFADIFCETG
                     VFTIEQSQHYMQKAKEAGFKVKIHADEIDPLGGLELAIDEQAISADHLVASSDKGKEK
                     LRNSDTVAVLLPATTFYLGKEDYADARGMLDNNGAIALATDYNPGSSVTNNLQLVMAI
                     AALKLKLSPSEVWNAVTVNAAKAIDINAGTINTGDKANLVIWDAPNHEYIPYHFGINH
                     AEKVIKDGKVIVDNTLSFKV"
     gene            complement(13136..14797)
                     /gene="hutU"
                     /locus_tag="PPY92_002928"
     CDS             complement(13136..14797)
                     /gene="hutU"
                     /locus_tag="PPY92_002928"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HDJ2626833.1"
                     /translation="MRKIQAKKGLSIECKGWEQEAVLRMLYNNLDPEVAERPEDLVVY
                     GGIGKAARNWEAFEAIEKTLRELESDETMLVQSGKPVAVFKTHEEAPRVLISNSVLVP
                     EWANWDHFNELDKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFAELGNQHFNGDLAGT
                     VTLTAGLGGMGGAQPLAITMNHGVAICVDVDETRVDKRIDTKYCDVKTADLDEALKLA
                     EEAKERGEGLSIGLVGNAVDIHQAILEKGFKIDIITDQTSAHDPLNGYVPQGYSVEEA
                     KVLREKDPKKYVELSQASMAKHVELMLEFQKRGAVAFDYGNNIRQVAFNNGVKNAFDF
                     PGFVPAYIRPLFCEGKGPFRFAALSGDPKDIERADEEMRKLFPENEKLLRWLDLAEEK
                     ISYQGLPSRIAWLGYGERAKMGLALNRLVRDGEISAPIVIGRDHLDAGSVASPNRETE
                     SMKDGSDAVGDWAVLNALINTAAGGSWISFHHGGGVGMGYSLHAGMVVVADGSERAER
                     RLERVLTTDPGMGVARHVDAGYDIAIQTAKEKGIHIPMIDKAGDK"
     gene            14957..15841
                     /locus_tag="PPY92_002929"
     CDS             14957..15841
                     /locus_tag="PPY92_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDJ2626834.1"
                     /translation="MKIIQLEYFLAIVKYNSFTKAAQFLHISQPSLTATIKKMEADLG
                     YDLFTRSTKDIKITEKGIQFYRYASELVQQYRSTMEKMYDLSVTSEPRIKIGTLESTN
                     QWIANLIRKHHSDYPEQQYRLYEIHDKHQSIEQLLNFNIHLAITNEKKTHEDIRSIPL
                     YEESYILLAPKETFKNQNWVDVENLPLILPNKNSQVRKHLDDYFNRRNIRPNVVVETD
                     RFESAVGFVHLGLGYAIIPRFYYQSFHTSNLEYKKIRPNLGRKIYINYHKKRKHSEQV
                     HTFVQQCQDYLYGLLEAL"
     gene            16023..16442
                     /locus_tag="PPY92_002930"
     CDS             16023..16442
                     /locus_tag="PPY92_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB1/FosB3 family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HDJ2626835.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
BASE COUNT         5418 a         3264 c         2340 g         5462 t
ORIGIN      
        1 gaactactat agcaggcttt tgcaactttg caaacaatta ttttgtgatt tttctaaaaa
       61 attaaaaaag ttgctcaagt accgttgagt cacatacaac ttcagctatt ctcggtaatt
      121 atgatataat tttaataata ttaaaaagga gtatatcaat gttaaaacaa atactgccac
      181 gtgctacaaa aatatcgata ctctttgctg tagcattttt catcataaat tatatcggta
      241 tggaaaaacc tgacatccta tatttagttg gaagaactat tattgcaaca cttgctttca
      301 tactcatttg tcttacatta tttactatta ttaattctcc agagcgcaag attaaactcg
      361 gtacgacttt accgattgca ttaattattg gtatcatttt cggcgccata tttttaacgg
      421 tacaaatcgg tgtcatcact gggttaatca tcggtgtgat tgctacgttt atttgggaat
      481 tgattgaaaa aaataaagga ggtcgttcat catgacaaca gcattaattg gtgtaattgt
      541 attaatctta ctaattatta gctttgtgcc aaactaccgt gctatgaaat cagcaaaaga
      601 acaaggaagt aatgcaacaa gagctacaat tatggtaggc atagatatta tcttaattgt
      661 acttatcatc gtgacattat tattaaaata tatgtaacta tttaaaccac cctatctcat
      721 acataaacat gaacgttctg ccatttcaca tacatatggt cgtccattta atgaggtaag
      781 gtggttttct cgttaaagtt taccttgtaa tagtaaaaaa tagccaattg ttgcgagtat
      841 taagtaaatc attgaaccaa caacaaaaga tcttacttgc acgcgaaatg gtgtctgttt
      901 gttaaatata ggaccaatca ccaaatttcc aacgcctaaa attactggac cagctagaaa
      961 aaacaatata atgtaaccca tcatgatacg cctcctattt cgtaaagaaa cgcgctacaa
     1021 tttgatcgta atcaatatct tttgctacat aatctacatt agaaaagtcc tgtgcgcttg
     1081 tcattttatt cgtattaaca ataacatcta atccagctgc aatcgcggct gttgcaccat
     1141 ttacagaatc ttcaatagcc aaacattgtg tcggcatata atttaattgt tgtactgcag
     1201 ataaatataa ttcagggtca ggtttaactt gttcaacatc ttctctacca acaactacct
     1261 ctatataatc gtctagacct aatgctttaa aggtgggcat tatttccgct ttcacactac
     1321 ttgtggcaat tgccatagga atgtgacgtt gctttaaaaa tgccatcaat gatttaatcg
     1381 tatcaatcat cggtaatgtt gtactagtaa gatgatgttc ttcataaagt ttatctttat
     1441 tttccgaacc aatcgcttta attaaatggt catgcaaatc tgtagctgct cctccaatag
     1501 aagaacgata aaaatcaatg cttataggat cggcattatg catctctaaa tgtttattaa
     1561 taacattaaa taaatgttgt tccgtatcta ttattgttcc atcgaaatca aatataactg
     1621 ctctatacat atatttactc ctctattttt tctctttatc aaagcttaac atagtccgat
     1681 gaccacttca ttctattagt gcaattataa cttttttgac caatactttg ctaaaatagg
     1741 ccctgttata ttatgaatga aactaaacac tgcgcctggt actgctgcaa ttggattaaa
     1801 atgcaatgct gctaatgaca cagctaaacc agagttctgc attccaactt caatactgac
     1861 tgctttttga tcttgtcgat ctaattttaa aagacgagct aaccaatatc caatcgtata
     1921 ccctaatacg ttatgtaaaa taactactaa aaatattaat aatcctgtag ttaagatttg
     1981 gtgcttactg ccacctacaa ctattgctaa aattaatgaa atagcaacaa ctgatataat
     2041 cggcaaagct gtagaagctt tttcagcaat tttacgatta ataatttgca aaacaatacc
     2101 taaagcaatt ggaattaata caacttgaac aactgaccac aacatactca ggaaagatac
     2161 ttccaaccat tcatttgcaa atagatatat taacgcaggt gtaacgaatg gcgctagcaa
     2221 cgtagagacc gtcgtaatag aaacagaaag tgctacgtta gctttggcta aataactcat
     2281 tacattactt gatgtcccac ccggacaaca tccaactaat attacgccaa cagcaatatc
     2341 aggtggtaaa tgaaaagact ttgcaattat aaatgctaat gtgggcataa tactgaattg
     2401 tagacagaca ccaataatta ctgctctagg tgctttaaag accattttga aatcattagg
     2461 tgtaattgtt aatcccatac ctaacataac aataccaagt aaataaggta cccacttacc
     2521 taatgtcgca acatgttgtg ggaaaataaa tccaataaca gctgctacta acatccatac
     2581 caaaaaactc ttcgtggcaa atatactcaa tttttgaaac attgttaaca catcctcgtc
     2641 gataatattg aaaaatatta ccataagacc agtaaggtta caattacttt tctgaaaatt
     2701 aagattattt agccattctc ttcttattgc aaatatttta tcactgataa acaattattt
     2761 taaacttcat tcaaaagtct tagtgaataa agaaaaaaag cgtggtaaaa tcaacttgaa
     2821 caaaattaag tcacctttat aaatattaaa cgtgtattta atgccatgta ttcctcggga
     2881 ggtcctcaca tgttaattga taaattcgaa acatacatca tcaacatcgc cggtttaaat
     2941 gaccgtacta ctagaaaaaa attaagtaag ttatgcaaaa gtgtacaatt ttgtgatgcg
     3001 ttgcaattct caataaacaa acagtttaat cagtatgtat tagaaattag cttaccaaag
     3061 caacaacttc catacttcat ttcattttta agttttcatc agtatagtat tttccaagta
     3121 ctttcaccaa agaaaataaa tgaattactt gattctgata atttatatca atctgcaaag
     3181 cgtttcgata tcaatattga tggcttgcaa gatgctttca ttaaagacaa agtcatcgat
     3241 attatgaata tgttccaaaa tcacactgat atcacttata cgttgaataa gtctcatgca
     3301 catattatat gtacgccaga aatatttgct aaattgttac atacgattgc aactagaaat
     3361 atcgacattc tatctgccaa ttatagatcg tcatctatga gcaaagctcg tatatcataa
     3421 taaaagacac atctatacga tgatcattta caatgattgc gtatagtgat gtgtcttttt
     3481 ttgtattatt tatcttcgga cgattgatcc tcaaccattt gttcaaatgc ttcacgaact
     3541 tgtggtactg tcatacctac tacaacttgg atactcttac cactctttac taatccgtgt
     3601 gccatttgtt gatgcgtgaa atattccgta tccgcaactt tactttcatc ataaacagtt
     3661 aagcgtaatc ttgttgtaca gttagtaaca tctttgatat tttctttgcc gcctaaaccg
     3721 tcgaggtaat acgcagcttt gtcttcgtat tcattacctg ttgaagcacg tttagaatct
     3781 acgctatcac cttttttatt tttgtaatct tgcttagaaa ataatttaac ttcctcttct
     3841 gttttcttac gtccaggtaa tggaatatca aatttcaaaa ttaagaatct gaataagaag
     3901 aagtaaatcg ctacaaatat cagaccgata ataacttgtg caacataagt accccaatgt
     3961 tctttaccta atggcaacca gtttgttgaa ataaagtcta acaagccacc acccatgtta
     4021 cctacaacac caaatgcgta cattaaagta tccattgatg ctgctaaaac tgcgtgtaaa
     4081 acaaataagt atggtgcgat gaataagaat gtaaattcta atggctcagt aatacctaca
     4141 acgactgctg ttaatgttgc aggaataagt aatgctgcta cttttttacg attttcttta
     4201 ggtgtcgtag catacattgc taaagcaata cctaaacatc caaatacttt cccatttcct
     4261 tgaagcataa agccataagg gaattgctct tttaatggct tagtactctt agcaaactca
     4321 tttaaatgtt gtaaccattc agctttcaag ccatgattga ctactactgg tccaacttca
     4381 attggtgcat aaataaagtg atgtaatcct gttggtatta atacacgttc taagaagtgg
     4441 tacagccaaa caccgatata gcctgaagcg atgataaagt gttgcattga accaataccg
     4501 tgctgaacag ttggccaaat aacacaagta attgctgcta atggtaacat cacaaaaaat
     4561 gaaattgtaa ccacaaatgt taaaccttgg aatacaccaa ccatttctgg taatcgttta
     4621 ctgtagtatc tattgtgaat ccacgtaata atagctgaaa taataatacc acctagaata
     4681 ttcgtatcca atgtggcaat acctgcaatt gattttaaac caggtacatt ttcaacgcct
     4741 ttttctaaat tagcgccaaa tgtatgtggc cattgcgtta aaatggcatt tataaatgta
     4801 ttaaacatta agtaacccat caatgctgca agcgctgcat gacctggtgc ttttttagct
     4861 aaagaaagtg gtaaacctac aacaaataca acttccatat gcgtaaaaat aacccagcca
     4921 ccagattcta tcactgacca aaatttaaac caaaacgtat gttgatctgc taaactcccc
     4981 atgattgtag gatttttaaa taatgtcgca aagccaagca ctatcccaaa gaaagcgaac
     5041 attaataccg gtacaatcat tgcactaccg aaacgcttta tcgcattcat cttctattcc
     5101 ctccatatca tctttcctaa caatacatct aattagattc atttataaat agatgtctta
     5161 ctatttaaat ataatatata gtaaacgctt acacacctac aacgacattg acgtattttg
     5221 aaagtatttt gtataatcag attatctttt catatagtga aaattttttc acgaccttat
     5281 atatgacatc gttgtatttg taatacattc gttttaaacg cataatcaaa cctatatcaa
     5341 tacacaaata tatataatga catacaagat tttaatgtaa taacgatcta tcacacattt
     5401 attttcaagg aggttgaata tgtttttaga tgaacacatt aatcgaaact ttgataaact
     5461 taatgataat gatttacata tcgctcactt tatcaataca catatagatg aatgtaaaaa
     5521 tatgaaaata caagatttag cgcaattcac acatgcctcg aatgcaacca ttcacagatt
     5581 tacacgcaaa ttaggttttg acggttatag tgattttaaa tcgtacctta aatttgaaag
     5641 taataagaca caccaattac cttctaactc tatcgatagt ttcaaacaag aaatcgatag
     5701 tacattcagt tatttagaac gcgttgatta ccagcttctt actgaaaaaa tgcatacagc
     5761 ttcaacaata tatttgtatg gtacagggcg tgcacagtta aacgttgctt cagaggcaca
     5821 gcgtatattg ttaacgttac ataatcatgt cattgtactt catgatgaac atgaacttaa
     5881 aatgattttg aacaagagtg aacctaatga tttattcttt attatttcat tatcaggtga
     5941 aacacatgaa ttattagaag taacaaactt gcttcaactt agacaaaaat actttatttc
     6001 tgttactaca atgaaagata atacccttgc tcaaaaagca aattacaacg tctatgtatc
     6061 cagtaacaca ttctacttaa ctgatggtac agactattct agcttcatca gttatcatat
     6121 attctttgaa acacttgtaa gaaaatataa tgagtattta ctacataatg atatttcaaa
     6181 ttagaaaaaa ggaagttatg atttaatggc cttgagccta tcataacttc cttttatcat
     6241 tttattgttg tgttgatgtt tcgataacgt ggtacatctt atcaaacatc aattcgaaac
     6301 catgcaccat ggcatcatga tattcttttt tcttttgctt gtattctaaa ttagtaaatc
     6361 gtctttcttt ttcaactaat gaacgataat aaaatagcat ttgggtgcca cctgtttcac
     6421 gttcaaaaaa ttctacctca atgacatctt gtgtttcact tagtccaggc ataccgatag
     6481 tcatcttaac gtattcatcc ataactaaag attcataaat gccttcaatc acatttactt
     6541 tgccattacg ttgttgatct acaatacgat atttaccgcc ttctttaacg tccgcttcaa
     6601 tctctttatt cgttctggct gatgtcataa accattgttt caacaaatct ttctttgtcc
     6661 aagcttcgta tactaactct ggagaaaatt tataaagctt ttcaatttca acttcgacat
     6721 gttcattctc tacattaaat tttgccactg ttgtccaccc actttcgctc ttacttttat
     6781 tttaacgtat ttttgctcag ttccaaacat agatgatcat catttttaaa agattagcgt
     6841 tatacggtga gtacaacatg atctgttaat ataacaagcc accttacttg gctacatcga
     6901 tatattgtta agcattaatg tttcatttct tgactagtgt tcttttttag ctttggaaaa
     6961 ttaaataaaa tcgcaataag tccgcataca cctaataata taggataaat gctgtatggg
     7021 aataacatta atggtgaaat accagctaca ccagccgctg aaatgacttg cgggctatat
     7081 ggtaataaac cttggaagca gcctccaaat atatcaagaa tacttgctga tttccttgaa
     7141 tctacatcat attcatctgc aatattttta gctaaaggac ctgacataat aatagagatg
     7201 gtgttgtttg ccgtggcaat atctgcgaca cttaccaaac tcgcaattcc taattctgcg
     7261 ccacgctttg atttcacttt agagcgaaca aattgcaaca accattcaat accaccattg
     7321 tgttgaataa taccgactaa accaccaatt agcaacgcaa tcatagcaat atcttccatg
     7381 cttataatac ctttggacac tgcatctagt agtcccatcc aaccgaatga accatctatg
     7441 agaccaatga ttccagctaa taatgttccg ccaatcaata cgataatgac atttacacct
     7501 aataatgcta ataccaatac taaaatatac ggtacaactt taattagatt ataatcatag
     7561 tttttagcat gatttaaaga aatgccattc gttaagaaat acagaataat aatcgttaaa
     7621 atagcacctg gcaatacaat tttaaagttt actctgaatt tatctttcat tttcgtatgt
     7681 tgtgttctaa ccgcagcaat tgttgtatct gaaatcattg atagattatc gccgaacatt
     7741 gcacctccaa caactgtagc cattgctagc gcagttggta catctgtcgc ttgtgcaaat
     7801 ccgtaaccaa caggtgctat tgcagcaaca gttcctacag acgtccccat agatatagaa
     7861 acaaacatac aaatcacaaa caatcctaca ataattaaat tttctgggat taatgatagt
     7921 cctaaattaa ctgtcgactt tacgccaccc attttttcag ctgtatttga aaatgcacct
     7981 gctaaaataa aaatcaacat cattaaaata atgtttgaat ggcctgcacc tttcgtgaag
     8041 acctcaactt ttttagcaaa tgattctttt cgattcatta ataacgccac aattaccgtt
     8101 atcgtaattg caacatttaa tggcattgaa gtaaaatcac ctgtgataat acctacgcct
     8161 aaaaacaacg ccacaaataa taacaagggg aataatgccc aagcattgct ctttttatgt
     8221 acttccatcc tttttacctg ctttccaatt aaaaatacct ctttctcaca aacgatgaag
     8281 aaagaggttt tcatgtgctt tacctgctta tcttcaaacc attacggtta ctggaattgg
     8341 cacattcgag atgttgccga ggcttcatag ggccagtccc tccacctctc tagataagtg
     8401 atgcttattt acgtttacgt tacaagataa tccttagtac gtcaatcata aattaatcag
     8461 gagtcgtata atatttttca taaacaatca ttgctactgt aataataatc aaaacaataa
     8521 tgctaataac aagtaaaagc caccatttaa gcattaatgc aataaaaatg aacacgatag
     8581 acacacttac taatattaat gatatgactt taaattgctg aacacgttgc ttggagatga
     8641 ctttcaactg tttgtttgat agacgcgtat tttttatact gattcccagt atattttcta
     8701 atatttgaac caatacgata cttattgcaa atataataat tggtaaaaca tcatagctcc
     8761 ctatagttaa tgtataaatt acaaatccaa tgtaaagtaa ccctgagaca aaggataaaa
     8821 agtatgcgac gtatttgtta aacttaatga tatgcttttt aacgttttga tgggtaaacc
     8881 atacattcat aataatcgca actgctacaa ataatgtgaa tactatatat aatggtaatt
     8941 tttgttcagg aaaaacagtc gctattccaa aagctaatgc taaaatcaaa aataatatag
     9001 ctctagatac tattaatgcc ataataacaa cccctttgtt taatatcgag tttgcaaatt
     9061 tacgtttatc agcatttcta tgatcagtac ttctacgggt agcgtttcta tgtaatttac
     9121 atcatcttaa catataaata cttcgctatt taattgaaaa catatcttat tattctttgt
     9181 ccgttctgac atttaatatc taaccttagg catttcactt gttaataaat ttaactttct
     9241 tccactaacc gtccctaaac ccaatcccgc aacagttttt aactttttcg ttgttgtcct
     9301 gacatcctca ttaagaaagt ttattctgct taaaacttat aatccacacc ctgagcaaac
     9361 gctccttatg acagagtatt aaaataagcc gataaagata cacaccttta ccgactattt
     9421 aaaatacact tcaccaattc attttaattt aatggattga agtaactaaa ttaatattat
     9481 gctgttcaat taaaagcttc atacaaccct aatctatttg cactccaccg ctaacaccga
     9541 acacttgtcc agttgtataa cttgattcct ctgacgctaa tagcacataa gttccacata
     9601 actcaacagg ttgacctgca cgacctaaag gtgttttttg accaaatgtt gggattttac
     9661 tttgaggttg cccaccagaa atttgtaatg gtgaccagaa tggaccaggt gctacacagt
     9721 tcactctaat tccttttggt cctaattctt ctgaaaaact tttagttaat gaaataattg
     9781 ctgcttttga agcggcataa tcatgaagaa taggactagg attataacct tgtacagatg
     9841 atgtcgttgt aattgacgca cccggtttta aatattccaa tgctttttga actgtccaaa
     9901 atagcggata gacattcgtt tcaaatgttt ctgtaaatgc ctcagttgta aatccatgaa
     9961 tatcatcatg atactgttga tgtccagcaa ctaaagtaac attatctaag ccacctaatt
    10021 gttgatatgc ttgttcaaca aggtcatagt tgaactgctc atctcttata tcaccaggaa
    10081 ttaacactgc cttttgacca ctttcttcaa ttacttggcg tacttcttgt gcatcttgtt
    10141 cttcactcgg aagatagtta atcgctacat ctgcaccttc tttagcatac gcaattgctg
    10201 ctgcacgccc tattgctgag tcaccacctg tgactaacat tttatagcct tgtaagcgtt
    10261 gatgaccttg gtaagacgtt tcgccacaat caggtgctgg cgtcatttca gattgtaaac
    10321 ccggtacctc ttgttcttgt ttttcatatt ccgttgtttt aaattttgtt ctaggatctt
    10381 gagttgccat tttttaacat ctccttattc gcttaatggt tattatttac ccaatcttcc
    10441 taggaactta atcatgatta cactaaaaat tactttcttc tttataaaaa caagctcgaa
    10501 ttactcatgc aatagtctct ttacaaattc aacaaaatac tcaggtactt tttccagaat
    10561 cctttcatcc ggtttatatt gagggtgatg taaatcatat tcactatgag aaccaattaa
    10621 cgcaaataca cttggaaaat gttgactata acctgaaaaa tcttctccaa tcgtaagcgg
    10681 ctgttccatc atttccacct tatatccaac atgttgggct actgcaattg ctttatgcgt
    10741 caatgcctca tcattcatca cagcgccagg taaatgtgta taatttaaat taattttcat
    10801 attatatgct tgagccaatc cgtccgcaat atcttgtaat cgtgtttcta caagctttcg
    10861 taccacagga tcaaaactac gcactgtgcc ttgtacatac gcatgatcag caatgacatt
    10921 ccaagtatta ccacatgata tttgtccaat tgttactacc gcttcatcaa acgcagatag
    10981 atttctacta actatggatt gaatactatt aatcaattgc gccaacacaa taactggatc
    11041 gttgcattgt tctggctttg cagcatgacc acccacgcct ttaatatgaa actcaaagcg
    11101 atctactgct gatgtaattg cccctgtttt gattgcaaat gtacctaccg aacgcgatgg
    11161 gtcattatga aaacccaata ctgcttgtac atcttttaat gcatctgttt caataatttt
    11221 aaaagcgcca tgtcctagtt cttctgctgg ttgaaaaatg aatttaacac gcccagtaag
    11281 agtgccctca atttctttta attttacagc cgtagccaaa atactagcca tgtgaatatc
    11341 atgaccgcac gcatgcataa caccttcatt ttcagattta aaatcctgtt caacaagctc
    11401 ttgaatcggt aaagcatcaa tgtcagcccg aacagctata caactcagac cttgtcccac
    11461 ttcagcaacc agcccagtca taagcggtaa gtctaatatt ctaatatgat gttctgttaa
    11521 aatatcttta attttttgtg tagtcttaaa ttctttatcg gatagttctg gaaattgatg
    11581 aaaatacctt ctccaggtaa cagcttgatc ttttaatccc atcggtcatt ccccttcctt
    11641 aagtcaatga tatgttgtct accctacgat gatcatcttt gactattaaa cgatgatttc
    11701 acaacaatgt actcttgtta attactttcg ctaatgatag acagttgttt aataatatcg
    11761 taacactgtt gtcaaactat tctaactttt ataattaaga atctatacaa aaacgtgttc
    11821 tcggataaac ttgtttttac aaaccacaaa agctctaaac attagtgtaa accaatgctt
    11881 agagctttct aattatttta tactttaaaa gataatgtgt tatctacgat gaccttaccg
    11941 tctttaataa ctttttctgc gtgattgata ccaaaatgat atggaatata ttcatgattt
    12001 ggtgcatccc aaattactaa attagcctta tcacctgtgt taattgtacc cgcgttaata
    12061 tctattgctt tagcagcgtt gaccgtaaca gcattccaca cttcactagg cgaaagcttt
    12121 aattttaatg ctgcaatggc cataacaagt tgcaagttat tcgttacact gctgcctgga
    12181 ttataatcag tagctaacgc gatggcacca ttattatcaa gcatacctct cgcatctgca
    12241 taatcttctt tacctaaata gaacgtcgtt gcaggtaaga ggacagctac agtatcacta
    12301 tttcgcaact tttcttttcc tttatcacta gaagctacta agtggtctgc tgatattgct
    12361 tgttcatcaa ttgctaattc cagtccgcct aacggatcaa tttcatccgc atgtattttc
    12421 actttaaaac ctgcttcttt ggctttttgc atataatgtt gcgattgttc tattgtaaat
    12481 acacctgttt cacagaaaat atccgcaaag tctgcatatt gttttacttc cggaagtaac
    12541 gcaatcattt cttctaaaaa tgcctcattt gaacttgcct ctttaggtac agcatgaggc
    12601 cctaggaaag tatgtttcat gtctaaatca tatttctcag ctaaacgatt agacactttc
    12661 aattgcttca gttcattttc tctatctaat ccataaccac tcttactttc aactgcaagc
    12721 acgccgtgtt taatcatagt aagcaaatca tgctctgctt ttttaaacaa gtcatcttcg
    12781 gatgtttctc tagtagcatt aacggtagat aatatgccac cacccatttc taatatttca
    12841 aggtaagact taccttgacg ttttaatgac atctcatgtt ctcgagatcc accaaatgtt
    12901 aaatgggtat gagcatctac taatgccgga gaaactactt ttccactagc atctatcgtc
    12961 tcagtcgcat catagtcatc tgtatgttgt ccagcatata caattttgcc atctttaatg
    13021 acaactgtac catttttcac aacatttaat tcatctaatt ccttaccctt caaaggttta
    13081 tctgttgatt tcggtaaaat taattctgct atgtgattaa ttattaaatc attcattatt
    13141 tatcacctgc tttatcaatc attggaatat gaataccttt ttctttagct gtttgaatag
    13201 cgatgtcata gccagcatca acatgtcggg caacacccat acctggatca gtcgtcaata
    13261 cacgttccaa tcttctttca gcacgctctg atccatctgc tacaacaacc atacccgcat
    13321 gaagtgaata tcccatgcca acaccgccac cgtgatggaa tgaaatccat gaaccacctg
    13381 cagctgtgtt aatgagtgca ttcaatacag cccaatcacc aaccgcgtca ctgccatctt
    13441 tcatactttc tgtttcacgg ttaggactag caactgaacc agcatctaaa tggtctcgtc
    13501 caataacaat tggtgctgaa atttcaccgt cacgtacaag acgatttaaa gctaagccca
    13561 ttttcgctct ttctccatag cctaaccaag caatacgtga tggtagtcct tgatatgaaa
    13621 ttttttcttc agctaaatca agccatctta ataacttttc attttctggg aaaagtttgc
    13681 gcatttcttc atccgcacgc tcgatatctt ttggatcacc actcaacgca gcaaagcgga
    13741 atggcccttt accttcacag aataatggtc taatgtaagc tggtacaaag cctgggaagt
    13801 caaaagcatt tttcactccg ttattgaagg ctacttgacg aatattgtta ccataatcaa
    13861 atgctacagc gccacgtttt tggaattcaa gcattaattc aacatgcttt gccattgaag
    13921 cttgtgacag ttcaacatat tttttcggat ctttttcacg caatactttc gcttcttcta
    13981 cagagtatcc ttgtggcaca tatccattta gcggatcatg tgcacttgtt tggtcagtaa
    14041 taatgtcaat tttaaatcct ttttctagaa tcgcttgatg gatgtctaca gcatttccaa
    14101 ctaacccgat tgataatcct tctccacgtt ctttcgcctc ttctgctaat tttaatgctt
    14161 catctaaatc agctgtttta acatcacagt atttcgtatc aattcgctta tcaacacgtg
    14221 tttcatcaac atccacgcaa attgctaccc catgattcat agtaattgct aacggttgcg
    14281 caccacccat accacctaaa cctgctgtca gtgtaacagt gcctgctaaa tctccattaa
    14341 agtgttgatt acctagctcg gcaaatgtct cataagtacc ttgcacaata ccttgagaac
    14401 caatatatat ccaactaccg gctgtcatct gtccatacat gattaaacct tttttatcta
    14461 attcattaaa atgatcccag tttgcccatt caggcactaa tactgaattt gaaattaata
    14521 cacgtggcgc ttcttcatgt gttttaaata cagcaactgg ctttcctgat tgtactaaca
    14581 ttgtctcatc tgattctaat tctcgtaacg ttttctctat tgcttcaaaa gcttcccaat
    14641 tacgtgctgc ttttccaata ccaccataaa caactaaatc ttctggtctt tcagcaactt
    14701 ctgggtctaa attgttgtat aacattctaa gtactgcttc ttgttcccaa cctttacact
    14761 caatactcaa accttttttt gcttgaattt ttctcataaa attcgctcct gttcttttaa
    14821 gaagttaatt ccactaaatt taaaacgctt acattattat cttcaatatt cattatagta
    14881 tgttaaaata tagccaacaa atataaataa actaattatc catagcttga acctataaat
    14941 aaaaggagca aaacacatga aaattattca gttagaatac ttcttggcta tcgtgaaata
    15001 taatagtttt actaaagctg cacaattttt acatattagc cagccatctt taactgctac
    15061 gattaaaaaa atggaagcag atttaggtta tgacttattt acacgttcaa caaaagacat
    15121 caagattacc gaaaaaggaa tacagtttta tcgttatgcg agcgaattag ttcaacaata
    15181 tcgatccacg atggaaaaaa tgtatgattt aagcgttaca tcagaaccaa ggataaaaat
    15241 tgggactctt gaatctacga atcaatggat tgcgaattta attcgaaagc accattccga
    15301 ctaccctgaa cagcaatatc gtttatatga aatacatgac aaacatcaat ctatagagca
    15361 attactgaat tttaatattc atttagctat aacaaatgaa aaaaaaaccc acgaagatat
    15421 aagatccatt cctttatatg aggaatctta cattttatta gcacccaagg aaacatttaa
    15481 aaatcaaaat tgggtagatg ttgaaaattt gccactcata ttaccaaaca aaaattctca
    15541 agtgcgcaaa cacttagatg actattttaa tagaagaaat attcgtccaa atgtcgttgt
    15601 agaaacagat cgattcgaat cagcagttgg atttgttcat ctcggcttag gttacgctat
    15661 cattccgaga ttttattacc aatcatttca cacgtctaat ttagaatata aaaaaattcg
    15721 tccaaactta ggccgaaaaa tttatatcaa ttaccataaa aaacgcaaac actccgaaca
    15781 agtacataca ttcgtacaac aatgccaaga ttatttatat ggacttttag aggctcttta
    15841 acttaagtta ttagagcctc ttatgcagtt gcgtagtcaa ctgtatacct ttttccttta
    15901 acttaagtta ttagagcctc ttatgcagtt gctcagatca tcgtataaaa attaatgacg
    15961 tcatttcaaa aatcgataca aaaataattt attataaaaa ttctaagaaa gaagtgaagc
    16021 agatgttaaa atctattaat catatatgct tttcagtcag aaatttaaac gattcaatac
    16081 atttttatag agatatttta cttgggaaat tgctattgac tggtaaaaaa actgcttatt
    16141 ttgagcttgc aggcctatgg attgctttaa atgaagaaaa agatatacca cgtaatgaaa
    16201 ttcacttttc atatacacat atagctttca ctatagatga cagcgaattt aaatattggc
    16261 atcagaggtt aaaagataat aacgtgaata ttttagaagg aagagttaga gatattagag
    16321 atagacaatc aatttacttt actgaccctg atggtcataa gctagaatta catactggca
    16381 cacttgagaa cagattaaat tattataaag aggctaaacc acatatgaca ttttacaaat
    16441 aaggcgtcat tataaaaagg cctcttgaaa tccgttaaaa tttt
//
LOCUS       DANBWH010000450        12090 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.guided.072, whole genome shotgun sequence.
ACCESSION   DANBWH010000450 DANBWH010000000
VERSION     DANBWH010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12090
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.guided.072"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(69..773)
                     /locus_tag="PPY92_002931"
     CDS             complement(69..773)
                     /locus_tag="PPY92_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HDJ2626836.1"
                     /translation="MYNEILGLVTFIATFVLMVLMYRFFGKQGLIAWVAIGTIIANIQ
                     VIKTVEIFGISATLGNVMFASIYLATDILNDIYGRRVAKRAVWLGFSSTLIMIIVMQL
                     SLHFIPAPEDMAQKALHAIFDVVPRIALGSIVAYIIGQHIDVFIFSLIKKVFSSDKTF
                     FIRAYGSTFLSSIIDTALFVAIAFIGSLPGTVVFEIFITTYVLKLASTVFNVPFGYIA
                     KSFYRKGKIDKLDQGY"
     gene            1018..1212
                     /locus_tag="PPY92_002932"
     CDS             1018..1212
                     /locus_tag="PPY92_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HDJ2626837.1"
                     /translation="MSRILTFSEQNTLAKIDDLMNTYCNQCPIKTRLRKLEGKTKAHH
                     FCINECSIGKEIKQLGNELQ"
     gene            1224..1475
                     /locus_tag="PPY92_002933"
     CDS             1224..1475
                     /locus_tag="PPY92_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HDJ2626838.1"
                     /translation="MKIISISETPNHNTMKITLSESREGMTSDTYTKVDDSQPAFIND
                     ILKVEGVKSIFHVMDFISVDKENDANWETVLPKVEAVFE"
     gene            1513..2637
                     /locus_tag="PPY92_002934"
     CDS             1513..2637
                     /locus_tag="PPY92_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor C"
                     /protein_id="HDJ2626839.1"
                     /translation="MEILRIEPTPSPNTMKVVLSYTREDKLSNTYKKVEETQPRFINQ
                     LLSIDGITSIFHVMNFLAVDKAPKADWEVILPDIKAAFSDANKVLESVNEPQIDNHFG
                     EIKAELLTFKGIPYQIKLTSADQELREQLPQTYVDHMTQAQTAHDNIVFMRKWLDLGN
                     RYGNIEEVMDGVLEEVLATYPESQLPVLVKHALEENHATNNYHFYRHVSLDEYHATDN
                     WKTRLRMLNHFPKPTFEDIPLLDLALSDEKVPVRRQAIVLLGMIESKEILPYLYKGLR
                     DKSPAVRRTAGDCISDLGYPEALPEMVLLLDDPQKIVRWRAAMFIFDEGNAEQLPALK
                     AHINDNAFEVKLQIEMAISRIENGDEALGSVWKQMANRTI"
     gene            2653..3090
                     /locus_tag="PPY92_002935"
     CDS             2653..3090
                     /locus_tag="PPY92_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HDJ2626840.1"
                     /translation="MNAYDAYMKEIAQQMRGELTQNGFTSLETSEAVSEYMNQVNADD
                     TTFVVINSTCGCAAGLARPAAVAVATQNEHRPTNTVTVFAGQDKEATATMREFIQQAP
                     SSPSYALFKGQDLVYFMPREFIEGRDINDIAMDLKDAFDENCK"
     gene            3514..4470
                     /locus_tag="PPY92_002936"
     CDS             3514..4470
                     /locus_tag="PPY92_002936"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDJ2626841.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYNGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            4670..5149
                     /locus_tag="PPY92_002937"
     CDS             4670..5149
                     /locus_tag="PPY92_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDJ2626842.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            5164..6003
                     /gene="fakB2"
                     /locus_tag="PPY92_002938"
     CDS             5164..6003
                     /gene="fakB2"
                     /locus_tag="PPY92_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HDJ2626843.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQA
                     SQMVDANVTVIDSKSISFSLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSIGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            6089..6622
                     /gene="msrA"
                     /locus_tag="PPY92_002939"
     CDS             6089..6622
                     /gene="msrA"
                     /locus_tag="PPY92_002939"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDJ2626844.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHVDNPTYE
                     QVCTNKTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DEHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            6615..7043
                     /gene="msrB"
                     /locus_tag="PPY92_002940"
     CDS             6615..7043
                     /gene="msrB"
                     /locus_tag="PPY92_002940"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDJ2626845.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMVRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            7055..7555
                     /locus_tag="PPY92_002941"
     CDS             7055..7555
                     /locus_tag="PPY92_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDJ2626846.1"
                     /translation="MFKKLFGKGKEVQKDIAIYAPLTGEYVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEFINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
                     IDVTMN"
     gene            7555..7776
                     /locus_tag="PPY92_002942"
     CDS             7555..7776
                     /locus_tag="PPY92_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HDJ2626847.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDDLYEEYTEWLKF"
     gene            7968..9458
                     /locus_tag="PPY92_002943"
     CDS             7968..9458
                     /locus_tag="PPY92_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HDJ2626848.1"
                     /translation="MDDKQHTSSSDDERAEIATSNQDQETNSSKRVHLKRWQFISILI
                     GTTLITAVITVVAYIFINQKISGLNKTDQANLNKIENVYKILNSDYYKKQDSDKLSKA
                     AIDGMVKELKDPYSEYLTKEQTKSFNEGVSGDFVGIGAEMQKKNDQIMVTSPMKGSPA
                     ERAGIRPKDVITKVNGKSIKGKALDEVVKDVRGKENTEVTLTVQRGSEEKDVKIKREK
                     IHVKSVDYKKKGKVGVITINKFQNDTSGELKDAVLKAHKDGLKKIVLDLRNNPGGLLD
                     EAVKMANIFIDKGKTVVKLEKGKDTEAIQTSNDSLKEAKDMDISILVNEGSASASEVF
                     TGALKDYNKAKVYGSKTFGKGVVQTTREFKDGSLLKYTEMKWLTPDGHYIHGKGIKPD
                     VTIDTPKYQSLNVIPNTKTFKVGDDDKNIKTIKIGLSALGYKVDNESTQFDQALENQV
                     KAFQQANKLEVTGEFNKETNNKFTELLVEKANKHDDVLDKLINILK"
     gene            9858..10367
                     /locus_tag="PPY92_002944"
     CDS             9858..10367
                     /locus_tag="PPY92_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDJ2626849.1"
                     /translation="MIRLGKMSDLDQILNLVEEAKELMKEHDNEQWDDQYPLLEHFEE
                     DIAKDYLYVLEENDKIYGFIVVDQDQAEWYDDIDWPVNREGAFVIHRLTGSKEYKGAA
                     TELFNYVIDVVKARGAEVILTDTFALNKPAQGLFAKFGFHKVGEQLMEYPPYDKGEPF
                     YAYYKNLKE"
     gene            10379..11449
                     /locus_tag="PPY92_002945"
     CDS             10379..11449
                     /locus_tag="PPY92_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDJ2626850.1"
                     /translation="MTKIAFTGGGTVGHVSVNLSLIPTALSQGYEALYIGSKNGIERE
                     MIESQLPEIKYYPISSGKLRRYISLENAKDVFKVLKGILDARKVLKKEKPDLLFSKGG
                     FVSVPVVIAAKSLNIPTIIHESDLTPGLANKIALKFAKKIYTTFEETLNYLPKEKADF
                     IGATIREDLKNGNAHNGYQLTGFNENKKVLLVMGGSLGSKKLNSIIRENLDALLQQYQ
                     VIHLTGKGLKDAQVKKSGYIQYEFVKEDLTDLLAITDTVISRAGSNAIYEFLTLRIPM
                     LLVPLGLDQSRGDQIDNANHFADKGYAKTIDEEQLTAQILLQELNEMEQERTRIINNM
                     KSYEQSYTKEALFDKMIKDALN"
     gene            11466..12080
                     /locus_tag="PPY92_002946"
     CDS             11466..12080
                     /locus_tag="PPY92_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDJ2626851.1"
                     /translation="MSQWKRISLLIVFALVFGIIAFFHESRLGKWIDNEVYEFIYSSE
                     SFITTSIMLGATKVGEVWAMLCISLLLVAYLMLKRHKIEALFFALTMALSGILNPALK
                     NIFDRERPTLLRLIDITGFSFPSGHAMGSTAYFGSGIYLLNRLNQGNSKGILIGLCAA
                     MILLISISRVYLGVHYPTDIIAGIIGGVFCIILSTLLLRNKLIN"
BASE COUNT         4505 a         1710 c         2136 g         3739 t
ORIGIN      
        1 ataaattttg cgaaaatatc atttctgtcc cactccctta aaacttattc ttttgtgtag
       61 taagtgcgtt aatagccttg atctaactta tcaatcttac ctttacgata aaatgattta
      121 gcaatatatc caaatggtac attgaaaact gttgaagcta attttaatac gtaagttgta
      181 ataaatattt caaatacaac tgtaccaggt aaacttccga taaaagcgat agctacaaat
      241 aaagctgtat caattattga gcttaaaaat gtactaccat atgcacggat gaaaaacgtt
      301 ttatcagaac taaatacttt tttaattagt gaaaagataa atacatcaat atgttgacca
      361 ataatatatg cgacaattga gcctaaagca atacgtggca caacatcaaa gattgcgtgt
      421 aatgcttttt gtgccatatc ttctggtgca ggaataaaat gtaatgacaa ttgcataaca
      481 ataatcataa ttaatgttga tgaaaagcct aaccaaactg ctctttttgc aactctacgc
      541 ccataaatat catttaaaat atctgttgct aaataaatag aagcaaacat gacattacct
      601 aaagttgctg aaataccaaa gatttctaca gttttaatca cttgtatgtt ggcaatgatt
      661 gtgccaattg caacccatgc aattaaacct tgtttaccaa aaaagcgata cataagtacc
      721 ataagtacga acgttgcaat aaacgtaact agtcctaaaa tttcattata catattaaaa
      781 tgtcctccta aattttgatc atgcgggtgt ttagaaaccg ctcaataaat aacttactca
      841 ttttactgaa aaatcgtttg cgatgcaata gcatttgaat ataaatacat tttacgtata
      901 gaattgtaaa aggtttcatt caaatcttag ggtcaaaaat gttataatat ttttatgtca
      961 aatttaaaac agtaacactt atttacaagg ttgcaatatt ttgaagtaat aaaggaagtg
     1021 tcgcgtattt taactttttc agagcaaaat acactcgcga aaatagatga tttaatgaat
     1081 acttattgca atcaatgtcc aatcaaaact cgtctgcgta aattagaggg gaaaacgaag
     1141 gcgcatcatt tttgtatcaa tgagtgttca atagggaaag aaataaaaca attaggaaat
     1201 gaacttcaat aggaggaagt caaatgaaaa ttatatctat atcagaaaca ccgaaccaca
     1261 acacaatgaa gattacactt agtgaaagca gagaaggtat gacatcagat acgtatacta
     1321 aagttgatga ttcacagcca gcatttatta atgacatctt aaaggttgaa ggcgttaaat
     1381 caattttcca tgttatggac tttatttcag tagataaaga aaatgacgca aattgggaaa
     1441 cagtattgcc aaaagtagag gctgtattcg aataaatttt tcatcaacta gtattcgggg
     1501 ggaataaagt atatggaaat tttacgtata gagccaacac caagtccaaa tacaatgaaa
     1561 gttgttttgt catatacaag agaagacaag ttatctaata cttataaaaa agtagaagaa
     1621 acacaaccaa gatttataaa tcagttgtta tctatagatg gtatcacttc catttttcat
     1681 gtcatgaact tcttagctgt tgataaggca ccaaaagctg attgggaagt catattacct
     1741 gatattaaag ctgctttttc tgatgcgaat aaggttttag aatctgtaaa tgaacctcaa
     1801 attgacaatc attttggtga aattaaagct gaattattaa cttttaaggg tataccgtat
     1861 caaattaagc taacttctgc tgaccaagaa ttaagagaac aattaccaca aacatatgtt
     1921 gatcatatga ctcaagcgca aacagcacat gacaatattg tttttatgcg taaatggcta
     1981 gatttaggaa atcgctatgg aaatattgaa gaagtaatgg atggtgtcct agaagaagtg
     2041 ctagctacct atccagaatc acagttaccc gtattggtaa aacatgcttt agaagaaaat
     2101 cacgcaacta ataattatca tttctatcga catgtctctt tggatgaata tcatgcaact
     2161 gataattgga agactcgatt acgaatgtta aaccattttc caaagccgac ttttgaagat
     2221 ataccgctac ttgatttagc tttatctgat gaaaaagtac cggttagacg tcaagcgatt
     2281 gtattattag gtatgattga aagtaaagaa attttaccgt atttatataa ggggcttcgt
     2341 gataaaagtc ctgctgtaag aagaacagca ggggattgca taagcgattt agggtatcca
     2401 gaggcactac cagaaatggt gctactatta gatgatccac agaaaatcgt taggtggcgt
     2461 gctgctatgt ttatctttga tgaaggtaat gcagagcagc ttcccgcact aaaagcccat
     2521 attaatgaca atgcgtttga agttaaatta caaattgaaa tggccatatc tcgtattgaa
     2581 aacggagatg aggcacttgg ttcagtatgg aaacaaatgg caaacagaac aatttaattg
     2641 gaggaattaa atatgaatgc atatgatgct tatatgaaag aaattgcgca acaaatgcgt
     2701 ggcgaattaa ctcaaaatgg ttttacaagt ttagaaacga gcgaagcagt atcggagtat
     2761 atgaaccaag taaatgctga tgacactact tttgtagtta ttaactctac atgcggctgt
     2821 gcagctggat tagcaagacc agctgcagta gcagttgcaa cacaaaatga acatagacct
     2881 acaaatacag ttacagtttt tgctgggcaa gataaagaag caactgctac aatgcgagaa
     2941 ttcattcagc aagcaccatc tagtccttcg tatgctttat tcaaaggtca agatttagtt
     3001 tattttatgc ctagagaatt tatcgaaggt agagatatta atgacattgc aatggactta
     3061 aaggatgcct ttgacgaaaa ttgtaaatag tacacataaa taaatataaa ggttaacaca
     3121 ttttataata ttaaaaatgg tgtctgtcat tgaaaataga gaatatagtt gtattctatt
     3181 tgttaaataa agtccgtttt taccaactat attttctaga aatttaactg ttttaatagg
     3241 acatcaaaca taatattcaa atcatgtgtt aacctctttt ttaaaatttt ttagcattaa
     3301 agttatagat ttgggtaaac aattaccaat tggaaacata tatcacgtta cgatgtggta
     3361 ggtacttaat cagcatttta taaataaagt aacggaattc atgatattaa tatcatattc
     3421 ctaaaatgag tgataacaaa atgctacata aaattaagtt atatcaaact aaatatacat
     3481 actataaata atgaaaatga ggtgttatcg catatgttga attcatttga tgcagcatat
     3541 cacagtcttt gtgaagaggt tttagaaata ggaaatacac gaaatgatcg cacaaataca
     3601 ggtacgattt cgaaatttgg tcatcaactt cgctttgact tatctaaagg atttccacta
     3661 ttaacgacaa agaaagtttc ttttaaatta gtagcaaccg aattattatg gttcattaaa
     3721 ggagatacaa acatccaata cttattaaaa tataataata atatttggaa cgaatgggct
     3781 tttgaaaatt atataaaatc agacgagtat aacggtccag atatgacaga tttcgggcat
     3841 cgtgcattga gtgatcctga atttaacgaa caatataaag aacaaatgaa acaatttaag
     3901 caacgtattc ttgaagatga tacttttgcg aagcaattcg gtgatttagg aaatgtttat
     3961 ggtaaacaat ggcgagattg ggttgataaa gatggtaatc attttgatca acttaaaaca
     4021 gtaattgaac aaattaagca taatccagat tcaagacgac acatcgtatc tgcatggaat
     4081 ccaacagaaa ttgatacaat ggcacttccg ccttgtcata ccatgttcca gttttatgtc
     4141 caagatggta agttaagttg ccagttatac caacgtagcg cagatatctt tttaggtgtg
     4201 ccatttaata tcgcaagcta cgctttattg acacacctta ttgccaaaga atgtggactt
     4261 gaagtaggag aatttgtgca tacatttgga gatgcacata tttattcaaa tcatattgat
     4321 gcgattcaaa cacaattagc acgtgaaagc ttcaatcctc caacattaaa aattaacagt
     4381 gacaagtcta ttttcgacat aaattatgaa gatttggaaa ttgttgacta tgaatcacat
     4441 ccagcaataa aagctccaat agcagtgtag tcattgcata gttagctaac catatagaca
     4501 tcaaaatgac atcatagtat tttcaagtgc aaaaaagtac ttttttgtgt taaacgtttt
     4561 cataaattat gcaaaatcat tatttctatc acactttatg ataaaaattg tgttaaatta
     4621 aagataactt agtaataaaa aatgaaatga tagaagaagg aggataatta tgactttatc
     4681 cattctagtt gcacatgact tgcaacgagt aattggtttt gaaaatcaat taccttggca
     4741 cctaccaaat gatttgaaac atgttaaaaa attatcaaca ggtcatactt tagtaatggg
     4801 tcgtaagaca tttgaatcga ttggtaaacc actaccgaat cgtcgaaatg ttgtacttac
     4861 ttcagataca agtttcaacg tagagggcgt tgatgtaatt cactctattg aagatattta
     4921 ccaactaccg ggccatgttt tcatatttgg agggcaaaca ttatttgaag aaatgattga
     4981 taaagtggac gacatgtata ttactgttat tgaaggtaaa ttccgtggtg atacgttctt
     5041 tccaccttat acatttgaag actgggaagt tgcctcttca gttgaaggta aactagatga
     5101 gaaaaataca attccacata cctttctaca tttaattcgt aaaaaataag ggggaaaacg
     5161 accatgacaa aacagattat agtaacagac tcaacatccg atttatctaa agaatactta
     5221 gaagcaaaca acattcatgt aattccttta agtttaacta ttgaaggagc ttcatacgtt
     5281 gaccaagtag atattacatc agaagaattt attaatcata ttgaaaatga tgaagatgta
     5341 aagacaagtc agccagccat aggtgaattt atatctgctt atgaagaact aggaaaagat
     5401 ggctctgaaa tcataagtat tcatctttct tcaggattaa gtggtacata taacactgct
     5461 taccaagcaa gtcaaatggt agatgctaat gtaactgtta ttgattcaaa atctatttct
     5521 tttagtttag ggtatcaaat acaacaccta gtagagcttg taaaagaagg tgtctcaact
     5581 tctgaaatag ttaaaaagtt aaatcattta agagaaaaca ttaaattatt tgtagttata
     5641 gggcaattga atcaattaat taaaggtggc agaattagta aaacaaaagg tttgattggt
     5701 aatcttatga aaattaaacc aattggtaca cttgatgatg gtcgcttaga gcttgtgcac
     5761 aatgcgagaa ctcaaaattc tagtatccaa tacttgaaaa aggaaattgc tgaatttata
     5821 ggagatcatg aaatcaaatc cattggtgtc gcacatgcta acgtcattga atatgttgat
     5881 aaattgaaga aagtttttaa cgaagctttt catgtgaata attacgatat aaatgtaact
     5941 acaccagtta tttctgcaca tactggtcaa ggtgcgattg gcctcgtagt ccttaagaag
     6001 taaatttaat cttttcagtg ttaattactt ccatttcaat cctttataga ctaaatttat
     6061 aattagatag atagaggagg taattcatat gacaaaagaa tatgcaacat tagcaggagg
     6121 atgtttctgg tgcatggtta aaccatttac atcatatcca ggcatcaagt cagtcgtatc
     6181 tggttatagt ggcggtcatg ttgataaccc tacttatgaa caggtatgta cgaataaaac
     6241 cggccatgtc gaagcagtac aaattacgtt tgatccagag gttacttcct ttgaaaatat
     6301 attagacata tatttcaaaa catttgaccc aactgatgat caagggcaat ttttcgatag
     6361 aggcgaaagc tatcaaccag tcattttcta tcatgatgag catcagaaaa aggctgctga
     6421 gtttaaaaag caacaattaa atgaacaagg tattttcaag aaaccagtga ttacacctat
     6481 taaaccatat aaaaatttct atccagctga agactaccat caagattatt ataaaaagaa
     6541 cccggtacat tattaccaat atcaacgtgg ttcaggtaga aaagcgttta tagaatcaca
     6601 ttgggggaat caaaatgctt aaaaaagata aaagtgaact aacagacata gaatatattg
     6661 ttacacaaga aaatggcact gaaccaccat ttatgaatga atattggaat cattttgcta
     6721 aagggattta tgtagataag atttctggta aacctttatt tacatctgaa gaaaagtttc
     6781 attctgaatg tggatggcct agcttttcta aagcgcttga tgacgatgaa attatagaat
     6841 tagtcgacaa atcatttggt atggtaagaa ctgaagtgcg ttcagaagaa tcaaatagtc
     6901 atttaggaca cgtctttaat gatggaccta aagaaagtgg cggcttaaga tactgtatca
     6961 attccgctgc aattcaattt attccatatg aaaaattaga agaattgggt tatggcgatt
     7021 taatatcaca ttttgataag taggagtgga aagtatgttt aaaaaattat tcggaaaagg
     7081 caaagaagtt cagaaagata tcgcaattta tgcaccatta actggagaat acgtgaaaat
     7141 tgaagatatt ccagatcctg tattcgcaca aaaaatgatg ggcgaaggtt ttggtattaa
     7201 tccaactgaa ggagaagttg tgtctccaat tgcaggacgt gttgacaatg tctttccaac
     7261 taagcatgcg attgggttaa aagcagataa cggattagaa ttattagttc atatcggttt
     7321 agacacagtt caattagacg gtgaaggatt tgaagtgtta gtgtctagtg gtgacgaagt
     7381 taacgtcgga gatccattag taagattcaa ccttgaattt attaataata acgctaaatc
     7441 tgttatttca ccaattataa ttactaacac tgatcaagca gcttcaatta atatttatga
     7501 tgaaaatgct gtgattaaag gtgaaacaaa agtgattgat gtgacaatga actaatgaaa
     7561 aactattcgt tttatcaatt tgtcatgaca gttcgtggtc gacacgacga taaaggtcgt
     7621 ctagcagaag agatatttga cgatcttgct ttcccaaaac acgatgatga ttttaacata
     7681 ctgtctgatt atattgagac acatggtgat ttcacattgc caatgtctgt atttgatgat
     7741 ttatatgaag aatatacgga atggctaaaa ttttaatata atttttaaaa ataatagtta
     7801 gaaccagggt gatgcaattc gttatcctgg ttttaattta aaataaacta agttgtgact
     7861 aaaaattaat caattatagt gaaatatggt gcgctatctt gcataaattg atatgattaa
     7921 ctacacagaa tttaaaagta cataatacat aataaggaag tgatacaatg gatgataagc
     7981 aacacacatc ttcatccgat gatgaacgcg ctgaaattgc aacaagcaat caagaccaag
     8041 aaactaattc atcgaaacgc gttcacttaa aacgttggca attcatatca atattaattg
     8101 gcacaaccct aataacagct gtcatcacag ttgttgcata tatttttata aatcaaaaaa
     8161 taagtggttt aaacaaaact gatcaagcaa acttaaataa aattgaaaat gtgtataaaa
     8221 tcttaaatag tgattattac aaaaaacagg actctgacaa gttaagtaaa gctgcaattg
     8281 atggtatggt caaagaatta aaagatcctt attctgaata tttaacaaaa gaacaaacga
     8341 aatcctttaa tgaaggtgtt tcaggtgatt ttgtaggtat tggtgcagaa atgcaaaaga
     8401 aaaatgatca aattatggtt actagcccta tgaagggttc tccagcagaa cgtgctggca
     8461 tccgtcctaa agatgtcatt actaaagtaa atggaaaatc aattaaagga aaagcattag
     8521 acgaagttgt caaagacgtt cgtggtaaag aaaacactga agtaacttta actgttcaac
     8581 gaggcagtga agaaaaagac gttaagatta aacgtgaaaa aattcatgtt aaaagtgttg
     8641 attataagaa aaaaggtaag gttggagtta ttactattaa taaattccag aatgatacat
     8701 caggtgaatt gaaagatgca gttcttaaag ctcacaaaga tggtttgaaa aagattgttt
     8761 tagatttaag aaataatcct ggtggactac tagatgaagc tgttaaaatg gcaaatattt
     8821 ttatcgataa aggaaaaact gttgttaaac tagaaaaagg taaagatact gaagcaattc
     8881 aaacttctaa tgactcgtta aaagaagcga aagacatgga tatatccatc ttagtgaatg
     8941 aaggttcagc tagtgcttct gaagtgttta ctggtgcgct aaaggactat aataaagcta
     9001 aagtttatgg gtcaaaaaca ttcggcaaag gtgtcgtaca aactacaaga gagtttaagg
     9061 atggttcatt gttaaaatat actgaaatga aatggttaac gccagatggt cattatattc
     9121 acggtaaggg cattaaacca gacgttacta ttgacacacc taaatatcaa tctttaaatg
     9181 tcattcctaa tacgaaaaca tttaaagttg gagacgatga taaaaatatt aaaactatta
     9241 aaattggttt atcagcttta ggttataaag ttgataatga atcaacgcaa tttgatcaag
     9301 ctttagaaaa tcaagttaaa gctttccaac aagcgaataa actcgaggta actggtgaat
     9361 ttaataaaga aacgaataat aaatttactg agttattagt tgaaaaagct aataaacatg
     9421 atgatgttct cgataagttg attaatattt taaaataagc gatacacact actaaaattg
     9481 tattattatt atgttaatga cacgcttcct aaatttgcaa agatagcaat ttaggaggcg
     9541 tgtttatttt tattgacgtc taaccctaaa agatataaat tagacattta caaatgatgt
     9601 aaataacgca atttctatca tcgctgttaa caattcatgc tttaatatac tatgagcata
     9661 tactttttaa atagtattat tcactagttt taacaatcga ttaattggta tatgatactt
     9721 ttattggtta ttttcatccc atagtgtgat aattactatt tttcattcat aataaaggtt
     9781 taaagcatgt taatagtgtg taagattaac atgtactgaa aaacatgttt aacataacga
     9841 tataaggagt gacgtacatg atccgtctag gtaaaatgtc agatttagat caaatcttaa
     9901 atctagtaga agaagcaaaa gaattaatga aagaacacga caacgagcaa tgggacgatc
     9961 agtacccact tttagaacat tttgaagaag atattgctaa agattatttg tacgtattag
    10021 aggaaaatga caaaatttat ggctttattg ttgtcgacca agaccaagca gaatggtatg
    10081 atgacattga ctggccagta aatagagaag gcgcctttgt tattcatcga ttaactggtt
    10141 cgaaagaata taaaggagct gctacagaat tattcaatta tgttattgat gtagttaaag
    10201 cacgtggtgc agaagttatt ttaacggaca cctttgcgtt aaacaaacct gcacaaggtt
    10261 tatttgcgaa atttggattt cataaggtcg gtgaacaatt aatggaatat ccgccatatg
    10321 ataaaggtga accattttat gcatattata aaaatttaaa agaatagagg taatattaat
    10381 gacgaaaatc gcatttaccg gagggggaac agttggacac gtatcagtaa atttaagttt
    10441 aattccaact gcattatcac aaggttatga agcgctttat attggttcta aaaatggtat
    10501 tgaaagagaa atgattgaat cacaactacc agaaattaag tattatccta tttcgagtgg
    10561 taaattaaga agatatattt ctttagaaaa tgccaaagac gtatttaaag tattgaaagg
    10621 tattcttgat gctcgtaaag ttttgaaaaa agaaaaacct gatctattat tttcaaaagg
    10681 tggatttgta tctgtgcctg ttgttattgc agccaaatca ttaaatatac caactattat
    10741 tcatgaatct gacttaacac caggattagc gaataagata gcacttaaat ttgccaagaa
    10801 aatatataca acatttgaag aaacgctaaa ctacttacct aaagagaaag ctgattttat
    10861 tggagcaaca attcgagaag atttaaaaaa tggtaatgca cataatggtt atcaattaac
    10921 aggctttaat gaaaataaaa aagttttact tgttatgggt ggaagcttag gaagtaaaaa
    10981 attaaatagc attattcgcg aaaacttaga tgcattatta caacaatatc aagtgataca
    11041 tttaactggt aaaggattaa aagatgctca agttaaaaaa tcaggatata tacaatatga
    11101 atttgttaaa gaggatttaa cagatttatt agcaattacg gatacagtaa taagtagagc
    11161 tggatcaaat gcgatttatg agttcttaac attacgtata ccaatgttat tagtaccatt
    11221 aggtttagat caatcccgag gcgaccaaat tgacaatgca aatcattttg ctgataaagg
    11281 ttatgctaaa acgattgatg aagaacaatt aacagcacaa attttattac aagaactaaa
    11341 tgaaatggaa caggaaagaa ctcgaattat caataatatg aaatcgtatg aacaaagtta
    11401 tacgaaagaa gctttatttg ataagatgat taaagacgca ttgaattaat ggggggtaat
    11461 gctttatgag tcaatggaaa cgtatctctt tgctcatcgt ttttgcattg gtttttggaa
    11521 ttatcgcgtt tttccacgaa tcaagactag ggaaatggat tgataatgaa gtttatgaat
    11581 ttatatattc atcagagagc tttattacga catctatcat gcttggggct actaaagtag
    11641 gtgaagtctg ggcaatgtta tgtatttcat tacttcttgt ggcatatctc atgttaaagc
    11701 gccacaaaat tgaagcatta ttttttgcat taacaatggc actatctgga attttgaatc
    11761 cagcattaaa aaatatattc gatagagaaa gacctacatt gctgcgttta attgatataa
    11821 caggatttag ttttcctagc ggtcatgcta tgggatcaac tgcatatttt ggaagtggta
    11881 tctatctatt aaatcgatta aatcaaggta attcaaaagg tattcttata gggttatgtg
    11941 cagctatgat tttattgatt tccatatcac gtgtatatct aggtgtacat tatccaacag
    12001 atattattgc cggcattatt ggtggcgtat tttgcattat tttatcaacg ttattactta
    12061 gaaataaatt aataaattaa atagtaaaaa
//
LOCUS       DANBWH010000451         8120 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.guided.125, whole genome shotgun sequence.
ACCESSION   DANBWH010000451 DANBWH010000000
VERSION     DANBWH010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8120
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.guided.125"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(42..488)
                     /locus_tag="PPY92_002947"
                     /pseudo
     CDS             complement(42..488)
                     /locus_tag="PPY92_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
     gene            595..2352
                     /gene="blaR1"
                     /locus_tag="PPY92_002948"
     CDS             595..2352
                     /gene="blaR1"
                     /locus_tag="PPY92_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HDJ2626852.1"
                     /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTML
                     AGLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNINTTKPIQEFTTDIHKFNWDSI
                     DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIV
                     IRKAEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFN
                     IFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTPFSN
                     KNILSHSFNGKKSLLKSRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQSL
                     TDYNYKKPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYK
                     IYLALFGLDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIP
                     KNYTAAQLKQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQ
                     LSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGK
                     NAELISEKILKGMGVLNDQ"
     gene            2342..2722
                     /gene="blaI"
                     /locus_tag="PPY92_002949"
     CDS             2342..2722
                     /gene="blaI"
                     /locus_tag="PPY92_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HDJ2626853.1"
                     /translation="MTNKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            2986..3579
                     /locus_tag="PPY92_002950"
     CDS             2986..3579
                     /locus_tag="PPY92_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDJ2626854.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNQKDLNLLKTLYDNG
                     TPIKTIAEQWHVSRTTIYRYLNKLEDKEDEKQGEVSN"
     gene            3551..4993
                     /locus_tag="PPY92_002951"
     CDS             3551..4993
                     /locus_tag="PPY92_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDJ2626855.1"
                     /translation="MKNKEKYLTNFSEAKRKKATQKYNIIKPFILGEQSLSSISKSKG
                     IALSTLYRWNKSYKEQGLKGLIYATKADKGTRKIEPKIIDEIERLALMNKRNSIATIH
                     RKTTNYCKENNFDIPSYKQIYSVIKAMPKSVIDFSHQGEKYYQNKYDLIQIRESSRPN
                     EIWQADHTLLDIFILDQKGNINRPWLTIIMDDYSRAIAGYFLSFDAPNAQNTALTLHQ
                     AIWNKNDTNWPVCGIPEKFYTDHGSDFTSHHMEQVAIDLKINLMFSKVGVPRGRGKIE
                     RFFQTVNQTFLEQLPGYINNNDTPSNLINFHNFEEKLRYFLIEDYNQKEHSTIHTTPI
                     SRWNSNHFFPNMPSSLEQLDLLLLEIPKSRKVHSDGIHFQGFRYSNTNLAAYVGEYVL
                     IRYNPNDMAEIRVFYRDEFLCTAICPEIADYSIDIKDIQHARSQRRKHLKQNIASPST
                     TELIKEEKNCGYSPQETTKNVKKLKRYRND"
     gene            4986..5744
                     /locus_tag="PPY92_002952"
     CDS             4986..5744
                     /locus_tag="PPY92_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDJ2626856.1"
                     /translation="MTKNQNFIETKEYKRFAEFCDACIKYQYIGICYGQPGVGKTLSS
                     RYYTNWDTIEKQVNHRGWEDLASKTTDDILSVDKIFYTAPAEKQSGKSIENRPVFQEV
                     LNFLRMGDRLIVESIDRLGRNYEEIIQTVNYLKDKEVQLMITSLPMMNEVIGNPLLDK
                     FMKDLIIQILAMISEQERNESKRRQAQGIQVAKEKGIYKGRPVLYSPNAKDPQKRLVY
                     YRVVELLEQGKSISTIAKEVGITRQTIYRIKNSK"
     gene            5805..6119
                     /gene="arsR"
                     /locus_tag="PPY92_002953"
     CDS             5805..6119
                     /gene="arsR"
                     /locus_tag="PPY92_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HDJ2626857.1"
                     /translation="MSYKELSTILKILSDPSRLEILDLLSCGELCACDLLEHFQFSQP
                     TLSHHMKSLVDNELVTTRKDGNKHWYQLNHAILDDIIQNLNIINTSNQRCVCKNVKSG
                     DC"
     gene            6116..7408
                     /gene="arsB"
                     /locus_tag="PPY92_002954"
     CDS             6116..7408
                     /gene="arsB"
                     /locus_tag="PPY92_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter membrane subunit
                     ArsB"
                     /protein_id="HDJ2626858.1"
                     /translation="MMTILAIVIFLLTLTFVIWQPKGLDIGITALIGAVVAIITGVVS
                     LSDVLEVTGIVWNATLTFVAVILISLILDEIGFFEWSAIHMVKASNGNGLKMFVFIML
                     LGAIVAAFFANDGAALILTPIVLAMVRNLGFNQKVIFPFIIASGFIADTTSLPLIVSN
                     LVNIVSADYFDIGFIEYFSRMIIPNIFSLIASILVLWLYFRKSIPKTFDTENLSDPKS
                     AIKDSKLFKLSWIVLAVLLVGYLVSEFIQIPVSIIAGIIAFIFVILARKSKAVHTKQV
                     IKGAPWNIVVFSIGMYLVVFGLKNVGITTVLGDVLTNISNYGLFSSIMGMGFIAAFLS
                     SIMNNMPTVLIDAIAIGQSSATGILKEGMVYANVIGSDLGPKITPIGSLATLLWLHVL
                     TQKGVKISWGTYFKTGIIITIPVLFVTLLGLYLTLIIF"
     gene            7426..7821
                     /locus_tag="PPY92_002955"
     CDS             7426..7821
                     /locus_tag="PPY92_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent arsenate reductase"
                     /protein_id="HDJ2626859.1"
                     /translation="MDKKTIYFICTGNSCRSQMAEGWGKEILGEGWNVYSAGIETHGV
                     NPKAIEAMKEVDIDISNHTSDLIDNDILKQSDLVVTLCSDADNNCPILPPNVKKEHWG
                     FDDPAGKEWSEFQRVRDEIKLAIEKFKLR"
BASE COUNT         3030 a         1078 c         1306 g         2706 t
ORIGIN      
        1 tgttaaaatt atactataaa ttcaactttg caacagaacc ctttatctcc tagttctttt
       61 agacgttgtt taactttttt gattccaccg atttctttta taattttatt gtttgctgta
      121 ttatcactat atgtcattga agcctcaata agttctttta aagtgatatc ttttcctaca
      181 tatttttcta aaataggaga ataagcaact atatcatctt tgttaatatg tactttttta
      241 tttaacttat tataaggtac ttgttctaac aaaatagcac tatttatcgc ttttgaagtc
      301 gaagcatagg caaatctctt atctgaatta aattttactt ccttaccact tttagtatct
      361 aaagcataaa caccaatatg agcattatat tttttttcta aatcatttaa ctctttggca
      421 tgtgaactgt ttgaattaca tgcacttaaa actaaagcaa ttacaattaa aaatattaac
      481 tttttcaaaa taaaccctcc gatattacag ttgtaatttt tataaatcaa taatattaca
      541 attgtaatat tgatgtcaat tgtttattat aaatatttga aagaaggtgt caaaatggct
      601 aaattactaa taatgagcgt agtaagcttt tgttttatat ttctattgtt agtatttttt
      661 aggtacatat taaaacgcta ttttaattat agtttaaatt ataaagtttg gtatctaact
      721 atgcttgctg gattaattcc ttttatccca attaaattct cttttataaa atttaataat
      781 gtgaataatc aagcgcccac agttgaaagt aagtcacacg atttgaacca taacataaat
      841 accaccaaac ctattcaaga gttcacaaca gatatccata agtttaattg ggattcaatt
      901 gataatatct gcacagttat ttggatagtt ttagttatta ttttaagttt taaatttttg
      961 aaagccttat tatatcttaa atatttaaag aaacagtcac tttatctaaa cgaaaatgaa
     1021 aaaaataaaa tagatacgat acttttcaac catcaatata aaaaaaatat tgtgattcga
     1081 aaagcagagg ctattcaatc tccaataact ttttggtatg ggaaatatat tattttgatt
     1141 cctagttcat attttaaaag tgtaattgat aaaagactaa aatatataat actgcatgaa
     1201 tatgctcatg ctaaaaatag agatacttta catttaatta tctttaatat cttctctatt
     1261 attatgagtt acaatccatt agtacatatt gtaaaaagaa agataattca tgacaatgaa
     1321 gtagaagccg atagatttgt ccttaataat attaacaaaa atgaatttaa aacttatgcg
     1381 gaatctatta tggactccgt attaaaaacc ccattttcca acaaaaatat attaagccat
     1441 tcatttaatg gtaaaaaatc attactcaaa agtagattaa ttaatataaa agaagccgat
     1501 ctgaaaaagc agtcaaaatt aattctaatt tttatttgta tttttacttt ttttatcatg
     1561 ataattcaaa gccagttttt aatggggcaa tccttaactg attataatta taaaaagccc
     1621 ttacaaagcg attaccaaat tttagatgaa agtaaaaatt ttggttcaaa tagtggatct
     1681 ttcgttatgt acagcatgaa gaaagacaaa tactatattt ataatgaaaa agaaagtaga
     1741 aaaagatatt ctcctgattc aacttacaaa atttatttag cactgtttgg acttgaccgt
     1801 catattataa gtgataaaaa ttcacgtatg tcatggaatc ataaacatta tcttttcgag
     1861 tcttggaata aggaacaaga tttaaataca gcaatgcaaa attcagttaa ttggtacttc
     1921 gaacgtatta gtaatcaaat accaaagaac tatactgcgg ctcaactcaa gcaattaaat
     1981 tatggtaatg aaaatttggg aagttataaa agctattgga tggaagatag tttgaaaata
     2041 tctaatcttg aacaagtaat agtttttaaa aatatgatgg aacaaaataa ccattttagt
     2101 aaaaaagcaa agaatcaatt atcttcttca ttattgatta agaaaaatga aaagtatgaa
     2161 ctgtatggga aaacaggtac aggtatagta aacgggaagt ataataatgg gtggtttgta
     2221 ggttacgtaa ttacaaatca tgataagtat tattttgcta cacatttatc agatggaaac
     2281 ccgtctggga aaaatgctga attaataagt gaaaaaatat taaaaggaat gggtgtttta
     2341 aatgaccaat aagcaagttg aaatatctat ggctgaatgg gatgttatga atataatatg
     2401 ggataaaaaa tcagtatcag ctaatgaaat tgtagttgaa attcaaaaat ataaagaagt
     2461 tagtgataaa acgattagaa cattaatcac aagactatat aaaaaagaga ttataaaacg
     2521 atacaaatca gaaaatattt atttttactc atcaaatatt aaagaagacg atattaaaat
     2581 gaaaactgct aaaacctttc ttaataaact gtatggaggg gatatgaaaa gtttagtgct
     2641 gaattttgcg aaaaatgaag aattaaataa caaagaaatt gaagaattgc gagacatttt
     2701 aaatgatatt agtaaaaagt aattagaggt atgtttcaaa cataatacat atgtaaacca
     2761 tacttgactc ttattaaatt agttttgtta atgacattta aaatatatta ttaaagatga
     2821 gagtgtccag aaaataacca ttttctggac actcttcttt tatgtctaaa taagtaagaa
     2881 ttacatagga ttatgtgttc aaaaacccaa caaaaaatct atgttcataa aagtccttgt
     2941 aattaagtta tggtacactg aagatgtata taggaggttt gtattttgaa aataggttat
     3001 gcaagagttt caactggatt acaaaattta aatttacagg aggatcgatt aaatacatat
     3061 ggttgtgaaa agatatttaa tgaccatatg agtggttcaa aaagtaaaag acctggttta
     3121 gataaagcaa ttgaatttgc gaggtcagga gatacgattg ttgtttggag attagataga
     3181 ttgggacgta atatggagga tttaatcaca ttagttaatg agcttaacga acgaggtgta
     3241 agtttccata gtttagaaga aaatataaca atggataaat caagttcaac tggacaatta
     3301 ttatttcatt tattcgcagc atttgcagaa tttgaacgta atttaatttt agagcgttct
     3361 tcagctggcc gaattgcagc acgtgctaga ggccgatatg gaggcagacc agaaaaatta
     3421 aatcaaaaag atttaaactt acttaagaca ctttatgata atggcacgcc aattaagaca
     3481 attgcagaac agtggcatgt ttcacgtaca acaatttatc gttacctcaa taaattggag
     3541 gacaaggaag atgaaaaaca aggagaagta tctaactaat ttttctgaag ccaaacgtaa
     3601 aaaagctact caaaagtaca atattataaa accttttatc ctaggcgaac aatctttatc
     3661 aagtatttct aaaagtaaag ggattgcgtt aagcaccctt tatagatgga ataagtcata
     3721 taaagaacaa ggacttaaag gactaatcta tgcgacaaaa gctgataaag gaacacgtaa
     3781 aatagaacca aagataattg atgaaattga acgccttgcg cttatgaata aaagaaatag
     3841 tatagccaca attcatagga aaaccactaa ttactgtaaa gaaaataatt ttgatatacc
     3901 aagttataaa caaatttata gtgttattaa agcgatgcct aagtctgtta ttgatttctc
     3961 tcatcagggt gaaaaatact atcaaaataa gtacgattta atacaaatta gagaatcctc
     4021 gagacccaat gaaatatggc aagcagacca tactttgcta gatatcttta tattagacca
     4081 aaaaggtaat atcaatagac catggctaac tattattatg gatgactata gtcgtgcaat
     4141 tgcaggctat ttcttaagtt ttgatgcccc caatgctcaa aacacagcat taacactaca
     4201 ccaagcaata tggaataaaa atgataccaa ttggccagtg tgtggtatac ccgaaaaatt
     4261 ctacacagat catggaagtg actttacttc acatcatatg gaacaagtcg ctattgattt
     4321 gaaaattaac ttgatgtttt cgaaagttgg tgttcctcgt ggtcgtggaa aaatagaacg
     4381 attctttcag acagttaatc aaactttctt agaacaactg cctggatata taaacaataa
     4441 tgatactcct agtaatctaa taaattttca caattttgaa gaaaaattac gttacttttt
     4501 aattgaagat tataatcaaa aagaacatag tactattcat accacgccta tcagtcgttg
     4561 gaatagtaat cactttttcc ctaatatgcc gagtagctta gaacaacttg atttattatt
     4621 gttggaaata cctaaatcta gaaaagttca ttcagatggc attcattttc aaggttttcg
     4681 atacagtaat actaatctag cagcttatgt aggtgaatat gtactgattc gttataaccc
     4741 aaatgatatg gctgaaatac gtgtgtttta tagagatgaa tttctttgta ctgctatatg
     4801 tccagaaata gctgattact ccatagatat aaaagacata cagcacgctc gtagtcagag
     4861 aagaaaacac ttaaaacaaa atattgctag tccaagtacc acggaattaa ttaaggaaga
     4921 aaaaaattgt ggttattcac ctcaagaaac gactaaaaac gtcaaaaaat taaagaggta
     4981 tcgtaatgac taaaaatcaa aatttcattg aaacgaaaga gtataaacgc tttgctgaat
     5041 tttgtgatgc ttgtattaaa tatcagtata ttggtatatg ttatggtcaa cctggtgtcg
     5101 gaaaaacttt atcgtctagg tattacacaa attgggatac tattgaaaaa caagttaacc
     5161 atagaggttg ggaagattta gctagtaaaa cgaccgatga catactatcg gtggataaaa
     5221 tattttatac tgcaccagca gaaaaacaat caggtaaatc tatagaaaac agacctgttt
     5281 ttcaagaagt attaaatttt ttaagaatgg gagatcgttt aattgttgaa tctattgata
     5341 gactaggaag aaattacgag gaaataattc aaacagttaa ttacttaaaa gataaagaag
     5401 ttcagctcat gataacgagt ttacctatga tgaatgaagt cattggaaat ccactactag
     5461 ataaatttat gaaagattta attattcaaa ttttagcaat gatttcagaa caagaaagaa
     5521 atgaaagtaa acgtcgacaa gctcaaggga ttcaagtcgc caaagaaaaa ggtatatata
     5581 aagggagacc tgttctgtat tctcccaatg ctaaagaccc acaaaagcgt ttagtttatt
     5641 accgagttgt tgaattactt gaacagggta aatctataag tactatagct aaagaagttg
     5701 gtattacacg tcaaactata tatagaataa aaaatagtaa ataaaataac atagacaata
     5761 atctatatag atgttaatct atttaatatc taggaggttt attcatgtct tataaagaac
     5821 tatcaacaat attaaaaatt ttatcagatc caagtaggtt agaaatatta gatttacttt
     5881 cttgtggtga gctatgcgct tgtgacttat tagaacactt tcaattctca caacctacac
     5941 taagtcatca tatgaagtca ttagtagata atgaattagt tacaacacga aaagatggca
     6001 ataaacattg gtatcaactt aatcatgcta ttttagatga tattatccaa aacttgaaca
     6061 tcattaatac atctaatcaa agatgtgtat gtaaaaatgt gaaatcaggt gactgttgat
     6121 gactatttta gcaattgtaa tttttctttt aactttaacc tttgtgattt ggcaaccaaa
     6181 aggtttagat attggtatta cagctttaat tggagcggtt gttgctatca ttacaggagt
     6241 cgtaagtctt tctgatgtat tagaagtaac aggtattgtt tggaatgcaa ctttaacttt
     6301 tgtggctgtt attcttattt cattaatatt agatgaaatt ggtttttttg aatggtctgc
     6361 gatacatatg gtcaaggctt caaacggtaa tggcttaaaa atgtttgttt ttattatgtt
     6421 acttggggca attgtagcag catttttcgc aaatgatggt gcagctttaa tcttaacgcc
     6481 tattgtatta gcgatggtaa ggaatctagg atttaatcaa aaagtgattt tcccctttat
     6541 tattgccagt ggttttattg ctgatactac atcacttccc ttaattgtaa gtaacttagt
     6601 taatatcgtt tctgcagatt acttcgatat tggatttatt gaatatttta gccgaatgat
     6661 cattcctaat atattctctc tgattgctag tattctcgtt ttatggttat atttcagaaa
     6721 atccatacct aaaacgttcg atacagaaaa tctatcagat cctaaaagtg caattaaaga
     6781 ttctaagtta tttaagcttt catggatagt attagcagta ctactcgttg gatatcttgt
     6841 tagcgagttt atccaaattc ctgtatcaat tattgctggt attattgctt ttatctttgt
     6901 aatattagct cgtaaatcta aagcagttca tacaaaacaa gtgattaaag gtgcaccatg
     6961 gaatatcgtt gtattctcta ttggtatgta tcttgttgtg tttggactaa aaaatgtagg
     7021 tattacaacg gtccttgggg atgttttaac aaacatttca aattatgggt tatttagcag
     7081 catcatgggt atgggcttta tagctgcttt cttatcttcg attatgaaca acatgccaac
     7141 tgttttaata gatgcaatag cgattggcca atccagtgct acaggaatat taaaagaagg
     7201 catggtttat gcgaatgtca taggttctga tttaggacct aaaattacgc caattggttc
     7261 tttagcaaca ttattgtggc tacatgtctt aacacaaaaa ggtgtgaaga tttcgtgggg
     7321 aacatacttt aaaactggaa ttatcatcac tattccagtc ctatttgtaa cactcttagg
     7381 tttatacctt acactaatca tattttaaga aaaggggctt taattatgga taagaaaaca
     7441 atttatttta tatgtacagg aaactcttgt cgtagccaaa tggctgaagg ttggggaaag
     7501 gaaatattgg gtgaaggttg gaatgtctat tctgctggta ttgaaacaca tggtgttaat
     7561 cctaaagcaa tagaagctat gaaagaagta gatattgata tatcaaacca tacgtcagac
     7621 ttgattgata atgatatttt aaaacaatca gatttggtcg taacgttatg tagtgatgca
     7681 gacaataatt gtcctatttt accaccaaac gttaaaaaag agcattgggg ttttgatgat
     7741 ccagcaggta aagaatggtc agaattccaa cgtgttagag acgagattaa attagctata
     7801 gaaaagttta aattgagata atcataaaca cctcttattt agttaataag aggtgttttg
     7861 ccatttaaag aacaaaattt gtgccatata caatatgccc tctctttttc tcattaacga
     7921 acgtttataa cgcaatcatg atgagagtat taatacagtt aattatttga aatactttag
     7981 tttacataat gttgaattat aggtagtgaa attgtattta ttatacattg tcatatcgtt
     8041 gtttaatgat agttaattta aggataatag aattaatatt atatatagat gaacaaaaca
     8101 agcaacagag caaaaaaaca
//
LOCUS       DANBWH010000452         6708 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.guided.151, whole genome shotgun sequence.
ACCESSION   DANBWH010000452 DANBWH010000000
VERSION     DANBWH010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6708
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.guided.151"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            134..1219
                     /locus_tag="PPY92_002956"
     CDS             134..1219
                     /locus_tag="PPY92_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDJ2626860.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSKEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            1216..3108
                     /locus_tag="PPY92_002957"
     CDS             1216..3108
                     /locus_tag="PPY92_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDJ2626861.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNVDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            3115..3492
                     /locus_tag="PPY92_002958"
     CDS             3115..3492
                     /locus_tag="PPY92_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDJ2626862.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            3643..4425
                     /locus_tag="PPY92_002959"
     CDS             3643..4425
                     /locus_tag="PPY92_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HDJ2626863.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(4551..5282)
                     /gene="erm(A)"
                     /locus_tag="PPY92_002960"
     CDS             complement(4551..5282)
                     /gene="erm(A)"
                     /locus_tag="PPY92_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HDJ2626864.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(5365..5424)
                     /locus_tag="PPY92_002961"
     CDS             complement(5365..5424)
                     /locus_tag="PPY92_002961"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HDJ2626865.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            5816..6478
                     /locus_tag="PPY92_002962"
     CDS             5816..6478
                     /locus_tag="PPY92_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDJ2626866.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
BASE COUNT         2410 a          909 c         1276 g         2113 t
ORIGIN      
        1 ttaaccatag tataataaat aatagtagtt aaagtagttt aagaggagat attaaaggga
       61 tattttactt taaataaaaa ttaacaaggt ttataatttg tagtagttaa agtatttaaa
      121 gaggtgggaa catgtgaagg ttcaaaggat agaagtggag aataagccgt atccattgta
      181 tttattacta gataaagaat accagctaat agaaccagta atgaaattta ttaaatactt
      241 agataatact ggtaagtctc ctaataccat taaggcatac tgctatcatt taaagttgct
      301 gtacgagttc atggaacaga gaggtgttat tcttaatgat attaactttg agttgttagc
      361 agacttcgta ggttggttga gatatccttc agcatcaaat gtaattgatc ttcagtcaaa
      421 aaaagccata agagaagaaa cgacagtgaa tacaatttta aatgtagtta tgagttttct
      481 tgattattta agtagattag gagaatttaa atcaattgat gtatttaaac aagcaaaggg
      541 aagaaatttc aaaggatttt tacatcatgt taataagggt agataccaaa agaatgtctt
      601 aaagttaagg gttaaaaaga aacagataag aacattgaga tcaaaggaag ttaagcaaat
      661 tattgatgct tgtcatacga aaagagataa attaatttta atgcttatgt atgagggtgg
      721 tttaagaatc ggtgaagtgt tatcgcttag gcttgaagat attgtcactt gggacaatca
      781 aatccattta acacctagag atgttaatgt taatgaagct tatattaaat taaggaagga
      841 aagaacaata catgtgagta aagaacttat gtcactttat acagattact tgatatatga
      901 gtatagtgag gaattggagc atgattatgt ttttatttcc ttaaaagaag gctattttgg
      961 gaaaccacta aagtaccaaa gtgttcttga tctagttaga agaatagtta aaaggactgg
     1021 aatagaattt acatcacata tgcttcgcca cactcacgca acgcagctaa ttagggaagg
     1081 atgggatgtt gcgttcgttc aaaagagatt aggtcacgca catgttcaga caacgttaaa
     1141 tacctatgtt catctttcag atcaggatat gaaaaatgag tttaataaat acctcgagag
     1201 aaaggagcat aagaaatgaa tgcttctagt aaaaggaaaa ttattagtca gagtgagatt
     1261 agcaaaaaaa tagctgtaat gaatgaagaa atgcaggggt tttgggctaa taatagttgg
     1321 gatataagaa aatgtccaca tccttctgcc atagaattaa gtaagaatcc tgctttaagg
     1381 aatcgttggg ttcgttttga acgtgttaaa aatctgtggt taagaacaga attgaaatat
     1441 ttttattttt accatttaaa caatggaata tggaatgcaa aaactgtctg gattagaaaa
     1501 ggaacagtaa ttaataaaat gttagatttc ttagatttaa agtatcctag cattacttca
     1561 attactgaag ttcctattga aaaagcaatg acggagtata gaacttattt gacaaaacgg
     1621 ggtgttagaa ttaccactac taattataag attactgcta atcaagaaaa aacacctgta
     1681 aaagctaatt cctactatgt tactaatcta aaacaattta tggagtttta tgagaacttt
     1741 tattttgatg gagaggagtg ggataaagac gtttgggata gacgtaactt acctttgcca
     1801 gatgataagg ttaacccaac acaatatgaa tatacaatta actttaaagg gtttcggaat
     1861 acatatttta aacaacttgt aaaaagatat tgtaagttga gattgaacgt ggatagcttt
     1921 tcctatgtaa gtgatattgc ccaaagactt aaagagttct ttaattttct ggacatgaaa
     1981 tttaaacaag ttcagagagt acaccaatta acgagagtgg aaattgaagc atatttaagt
     2041 gaactaaaca tgatgggaat aaaacctagt acaataactg ggaggatctc tatattggaa
     2101 ggactattta gtacccttct taggctagaa tgggatgatg ttccttccaa aatattaatt
     2161 tattctgagg actatccgaa aataccaaga gcaaaaccac gctttataga tgaattcgtc
     2221 ctagagcaat tgaacagtca tcttgataaa ttacccgaat atatagctac gatgactatg
     2281 attgttcaag aatgtggaat gaggataagt gaattgtgca ccttgaaaaa aggctgtcta
     2341 ttagaggaca aagatggaga tttcttttta aagtattatc aatggaaaat gaaaaaggag
     2401 catatagttc caatatctaa agaggtagct ttacttatta aagttcggga agataaagtt
     2461 tcagaggaat ttccagatag tgaatacctc tttccaagaa aagatggatc gccattaaaa
     2521 caagaaacat ttagaggtga gttaaataaa ttagcttatg agcaaaatat agtggataaa
     2581 tcaggtgaga tttatagatt ccatgcccat gcctttcgcc atacagtagg aacaagaatg
     2641 attaacaacg ggatgcccca gcatattgtg cagaaatttt tggggcatga aagcccagaa
     2701 atgacaagca gatacgctca tatctttgat gaaactctaa aaaatgaatt tactaaattt
     2761 caggaaaaac tggttaccaa taatggagat gtgcttgatc tagatgaaga taatgaagtc
     2821 gatgatgtag agcttcaatg gttcaagaaa aatataaatg cacaagtgct tccaaatggt
     2881 tattgtagat tgccagtagt agcaggtggt tgtccacatg cgaatgcatg cttagattgc
     2941 actcacttct gtaccagtaa gcaattctta ccacagcacg aagaacagtt agagcgtaca
     3001 gaagagttat tagccatagc taaggataaa caatggcaaa gacaagtaga gactaatagc
     3061 cgtgttaaag agcgtttaga acaaatcatt ggaagtttga cggggtaatt atcaatggat
     3121 aaacaagtta gaaatacaac agaaattgta cgtttggcga agcagaaatc aaaaaagaca
     3181 agggaaaaag tagacaaagc gatttctaaa ttttcgattg aaggtaaagt tattaatttt
     3241 aattcaatag caaaggaagc taatgtttct aaatcatggc tttataagga acacgatatt
     3301 aggcaaagaa tcgaatccct tcgtgagcgt caaataacag caaatgtagt ctcaaaaccc
     3361 aagaaaagtt ctcgttcgga ggaaatcctt attaaaacct taaaaagaag agtaatggaa
     3421 ttagaaaaag aaaataaaaa attacagaac caaattcaaa aattatatgg agatctgtat
     3481 aataaagaat aattattaat ctgtagacaa attgtgaaag gatgtactta aacgctaacg
     3541 gtcagcttta ttgaacagta atttaagtat atgtccaatc tagggtaagt aaattgagta
     3601 tcaatataaa ctttatatga acataatcaa cgaggtgaaa tcatgagcaa tttgattaac
     3661 ggaaaaatac caaatcaagc gattcaaaca ttaaaaatcg taaaagattt atttggaagt
     3721 tcaatagttg gagtatatct atttggttca gcagtaaatg gtggtttacg cattaacagc
     3781 gatgtagatg ttctagtcgt cgtgaatcat agtttacctc aattaactcg aaaaaaacta
     3841 acagaaagac taatgactat atcaggaaag attggaaata cggattctgt tagaccactt
     3901 gaagttacgg ttataaatag gagtgaagtt gtcccttggc aatatcctcc aaaaagagaa
     3961 tttatatacg gtgagtggct caggggtgaa tttgagaatg gacaaattca ggaaccaagc
     4021 tatgatcctg atttggctat tgttttagca caagcaagaa agaatagtat ttctctattt
     4081 ggtcctgatt cttcaagtat acttgtctcc gtacctttga cagatattcg aagagcaatt
     4141 aaggattctt tgccagaact aattgagggg ataaaaggtg atgagcgtaa tgtaatttta
     4201 accctagctc gaatgtggca aacagtgact actggtgaaa ttacctcgaa agatgtcgct
     4261 gcagaatggg ctatacctct tttacctaaa gagcatgtaa ctttactgga tatagctaga
     4321 aaaggctatc ggggagagtg tgatgataag tgggaaggac tatattcaaa ggtgaaagca
     4381 ctcgttaagt atatgaaaaa ttctatagaa acttctctca attaggctaa ttttattgca
     4441 ataacaggtg cttactttta aaactactga tttattgata aatattgaac aatttttggg
     4501 aagaataaag cgtcctcttg tgaaattaga gaacgcttta ttactttaat ttagtgaaac
     4561 aatttgtaac tattgaaaat agaaagaaat tgttccttcg atagtttatt aatattagtg
     4621 acatttgcat gcttcaaagc ctgtcggaat tggtttttag tgaaaagaac acgatattca
     4681 cggtttaccc acttataaac aaaagatcga tactttttgt agtccttctt tgaaatcaat
     4741 ggttgatgtc gttcaagaac aatcaataca gagtctacac ttggcttagg atgaaaatat
     4801 agtggtggta cttttttgag catttttata tccatctcca ccattaatag taaacccaaa
     4861 gctcgttgca gattttgcaa tcttttcgca aatcccttct caacgataag atagctatat
     4921 ttagcctgac tttcaaaggt aattcttttg acaatatccg tactgatgtt ataaggaata
     4981 ttaccatata tcttatagtt tatatgtttt gggaaggaaa attttagaat atccgtttga
     5041 atcactttta tattctcaga ggggtttacc gcttctttag tcacttgaca taagcctcca
     5101 tcaatttcta tagcagtaac tgatcgactc attttgacta gctctttggt aaaatgtcct
     5161 tttcctgatc cgatttctat tacgttgtct tgtttactga tattcgtgtg attcaatatt
     5221 tcttttacat gctttttaga agtaataaaa ttttgcgtgt ctttagggtt tttctggttc
     5281 attataacct tctccttact ggttcattat aaccttctcc ttactggtta taatgaactg
     5341 acattaagcg ttcattataa ccaattaatt ttgatttggt tgataacgaa ctttattaat
     5401 aacaaatata gaaaaagtac ccatactttt atctcctttc catttttttc attatgaatg
     5461 agataaagta atttctacta ctgcgatact ggtgcacata attgaaacct ccttatgtaa
     5521 ctgaatatga ttgtatcatc ttttcatttt agaataaacc tttatatgta attttgcact
     5581 tgacacttca gatggacgtt ttcttcaaaa taatatgttt tgaaacttga taagagaagc
     5641 cactgtcttt actaaaaagt aaaggctgcg ataaaggaca gtgactttaa taaagttgca
     5701 ctgttgtatt gtctttggat aaaattgtct aaagggaagt tttatcccca acgaaaaaac
     5761 atctaaagtg gtaagtaatg aaagaatgtc gataaagtag gaatggggga tctttttgaa
     5821 tacaatagaa tcacagtttg ataaggttgc agaagattac gatttcgtga atgagctttt
     5881 gaatgattat tcattctttg tgtctaatat gtctccaaag aaaggcagag cattagatat
     5941 cggatgtggc tcgggtttgt tagtggagaa attagcaagt tattatgatg aagtggtagg
     6001 gattgatatt tctaatcaaa tgctcgatct tgccaaatct aaacgtcaac taacaaatac
     6061 ggtctatctg aatatgaatg cagaacaact taattttaat gagaagtttg attttattgt
     6121 aagccgaaca acctttcatc atttggatga tatagccagc gtaatacagc aaatgaagga
     6181 actgttgaat gaggaaggaa gaatagttat tcttgataat gtatctgaag tcgagacacc
     6241 gcctacctat gtttataaat taggggcaat ccaagagttt ttaccgcatt gtttcaaatt
     6301 tggaataaaa aatgcgatta gaatttataa tcacaacaca tctaaatcat ggcttgagca
     6361 tttagcttca gataagtacc tatctgaaca aaattattac gatttatatg aaaagttgtt
     6421 gcctggatgc caattccata aaatgggttg ggcaatgggg gttgtctgga caaaataact
     6481 taaagctgtg gctttgaaaa gttgaatttg tgattgtttt cagagctatc gggtgcttta
     6541 gtataaataa caatgaataa aaaatcagcc tgtatcttat gttactatta agtaatttaa
     6601 gatacaggtt ttactatttt tgaaataagt accttccact tcaaatacat gtattcaaag
     6661 tgaaagtatt gatttaaatg attcttttgt aatttactct tgataaga
//
LOCUS       DANBWH010000453         6396 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.guided.159, whole genome shotgun sequence.
ACCESSION   DANBWH010000453 DANBWH010000000
VERSION     DANBWH010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6396
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.guided.159"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            221..289
                     /gene="psm-mec"
                     /locus_tag="PPY92_002963"
     CDS             221..289
                     /gene="psm-mec"
                     /locus_tag="PPY92_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-mec"
                     /protein_id="HDJ2626867.1"
                     /translation="MDFTGVITSIIDLIKTCIQAFG"
     gene            complement(374..745)
                     /gene="mecI"
                     /locus_tag="PPY92_002964"
     CDS             complement(374..745)
                     /gene="mecI"
                     /locus_tag="PPY92_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mecA-type methicillin resistance repressor MecI"
                     /protein_id="HDJ2626868.1"
                     /translation="MDNKTYEISSAEWEVMNIIWMKKYASANNIIEEIQMQKDWSPKT
                     IRTLITRLYKKGFIDRKKDNKIFQYYSLVEESDIKYKTSKNFINKVYKGGFNSLVLNF
                     VEKEDLSQDEIEELRNILNKK"
     gene            complement(745..2502)
                     /gene="mecR1"
                     /locus_tag="PPY92_002965"
     CDS             complement(745..2502)
                     /gene="mecR1"
                     /locus_tag="PPY92_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer MecR1"
                     /protein_id="HDJ2626869.1"
                     /translation="MLSSFLMLSIISSLLTICVIFLVRMLYIKYTQNIMSHKIWLLVL
                     VSTLIPLIPFYKISNFTFSKDMMNRNVSDTTSSVSHMLDGQQSSVTKDLAINVNQFET
                     SNITYMILLIWVFGSLLCLFYMIKAFRQIDVIKSSSLESSYLNERLKVCQSKMQFYKK
                     HITISYSSNIDNPMVFGLVKSQIVLPTVVVETMNDKEIEYIILHELSHVKSHDLIFNQ
                     LYVVFKMIFWFNPALYISKTMMDNDCEKVCDRNVLKILNRHEHIRYGESILKCSILKS
                     QHINNVAAQYLLGFNSNIKERVKYIALYDSMPKPNRNKRIVAYIVCSISLLIQAPLLS
                     AHVQQDKYETNVSYKKLNQLAPYFKGFDGSFVLYNEREQAYSIYNEPESKQRYSPNST
                     YKIYLALMAFDQNLLSLNHTEQQWDKHQYPFKEWNQDQNLNSSMKYSVNWYYENLNKH
                     LRQDEVKSYLDLIEYGNEEISGNENYWNESSLKISAIEQVNLLKNMKQHNMHFDNKAI
                     EKVENSMTLKQKDTYKYVGKTGTGIVNHKEANGWFVGYVETKDNTYYFATHLKGEDNA
                     NGEKAQQISERILKEMELI"
     gene            2599..4608
                     /gene="mecA"
                     /locus_tag="PPY92_002966"
     CDS             2599..4608
                     /gene="mecA"
                     /locus_tag="PPY92_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase MecA"
                     /protein_id="HDJ2626870.1"
                     /translation="MMKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFK
                     QVYKDSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKI
                     KTNYGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQSIHIENLKSERGKILDRNN
                     VELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTV
                     KKMDEYLSDFAKKFHLTTNETESRNYPLGKATSHLLGYVGPINSEELKQKEYKGYKDD
                     AVIGKKGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQ
                     KSIYNNMKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPL
                     LNKFQITTSPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV
                     NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKN
                     LDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISK
                     ENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKD
                     NPNMMMAINVKDVQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
     gene            complement(4654..5082)
                     /locus_tag="PPY92_002967"
     CDS             complement(4654..5082)
                     /locus_tag="PPY92_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HDJ2626871.1"
                     /translation="MKYDDFIVGETFKTKSLHITEEEIIQFATTFDPQYMHIDKEKAE
                     QSRFKGIIASGMHTLSISFKLWVEEGKYGEEVVAGTQMNNVKFIKPVYPGNTLYVIAE
                     ITNKKSIKKENGLVTVSLSTYNENEEIVFKGEVTALINNS"
     gene            complement(5179..5922)
                     /locus_tag="PPY92_002968"
     CDS             complement(5179..5922)
                     /locus_tag="PPY92_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HDJ2626872.1"
                     /translation="MLTVYGHRGLPSKAPENTIASFKAASEVEGINWLELDVAITKDE
                     QLIIIHDDYLERTTNMSGEITELNYDEIKDASAGSWFGEKFKDEHLPTFDDVVKIANE
                     YNMNLNVELKGITGPNGLALSKSMVKQVEEQLTNLNQNQEVLISSFNVVLVKLAEEIM
                     PQYNRAVIFHTTSFREDWRTLLDYCNAKIVNTEDAKLTKAKVKMVKEAGYELNVWTVN
                     KPARANQLANWGVDGIFTDNADKMVHLSQ"
BASE COUNT         2189 a         1005 c          897 g         2305 t
ORIGIN      
        1 cttaaaacct cacttaggtt atcatcttcg ttcttttatt ttttttatta tttcaataaa
       61 ttacgtattt ccaatatgac gattttttat gcaaagtcgt tgtatgtttt tcaaattttt
      121 gacatttatg caatctaaat agccaattag ggaattttta acatttttta tttgtttgat
      181 attataccta atgtatctta aatagaaaga gggtatgcat atggatttca ctggtgttat
      241 tacaagcatt attgatttaa tcaagacttg cattcaggct ttcggttaat tttttcaact
      301 aaaaaacaga ggaaatattc aacgacttga ttgtttcctc tgttttctat gtattgttgt
      361 aaacacaaca attttatttt ttattcaata tatttctcaa ttcttctatt tcatcttgtg
      421 atagatcttc tttttctaca aagtttaaga caagtgaatt gaaaccgcct ttgtatactt
      481 tattgataaa gtttttagat gttttatatt ttatatcact ttcttctaca agagagtaat
      541 attgaaaaat tttattgtct tttttacgat ctataaatcc ctttttatac aatctcgtta
      601 taagtgtacg aatggttttt ggactccagt ccttttgcat ttgtatttct tctattatat
      661 tattcgcact tgcatatttt ttcatccaaa tgatattcat aacttcccat tctgcagatg
      721 atatttcata cgttttatta tccattatat taactccatt tcttttaaaa tacgctcaga
      781 aatttgttgt gctttttcgc cattcgcatt gtcttcgcct tttaaatgtg tagcaaaata
      841 atacgtatta tctttcgttt caacataacc tacgaaccat ccatttgctt ctttgtgatt
      901 cacgattcct gttccagttt tacctacata tttataagta tctttttgtt tcaaagtcat
      961 actattttca actttttcaa tagccttatt atcaaaatgc atgttatgtt gtttcatatt
     1021 tttcaacaaa ttaacctgtt ctattgcaga aatttttaat gaagattcat tccaataatt
     1081 ttcattccct gatatttctt cattaccata ttcaattaga tctaaataag atttaacctc
     1141 atcttgtctt aaatgtttgt ttaaattttc gtaataccaa tttactgaat atttcattga
     1201 agaatttaaa ttttgatctt ggttccattc tttaaatgga tattgatgtt tatcccattg
     1261 ttgttcagta tgatttaatg agagtaaatt ttggtcgaat gccattaacg ctaaataaat
     1321 tttgtaagta gaattaggtg aatatcgttg tttactttct ggttcattat aaatagaata
     1381 agcttgctcc cgttcattat aaagcacaaa acttccatca aatcctttga aatacggagc
     1441 tagttgattt aattttttat atgatacatt tgtttcatat ttgtcttgtt gaacatgtgc
     1501 agatagtaac ggtgcttgta ttaaaagcga tatactacat acaatatacg caacaatacg
     1561 cttgtttcga ttaggtttag gcattgaatc ataaagtgca atatacttaa cacgttcttt
     1621 aatatttgaa ttaaaaccta gtaaatattg tgctgccaca ttatttatgt gctgagattt
     1681 taaaatagag cattttaata tcgattcacc ataacgtata tgttcatggc gattcaaaat
     1741 ttttaaaacg tttctatcac atactttttc acagtcattg tccatcattg ttttacttat
     1801 atatagtgca ggattaaacc agaatatcat tttaaaaaca acataaagct ggttgaatat
     1861 taagtcatga cttttcacat gtgatagttc atgtagaata atatattcaa tttctttgtc
     1921 attcatggtt tcgactacga cagttggtag tacaatttgg gatttcacta aaccaaatac
     1981 catcggatta tcaatgtttg aactataact aattgttata tgctttttgt agaactgcat
     2041 cttactttga catactttaa gtcgttcatt aagatatgac gattccaatg acgaactttt
     2101 aataacatca atttgtcgga atgccttaat catataaaat aagcacaaca aactaccaaa
     2161 tacccatatc aaaagaatca tatacgttat atttgaggtc tcaaactgat taacattaat
     2221 tgctaagtct ttcgtaacag atgattgttg accatctaac atatgactaa ccgaagaagt
     2281 cgtgtcagat acatttcgat tcatcatatc ttttgaaaat gtaaaattcg atattttgta
     2341 aaatggtatt aatggaatta acgtggagac gagcactaat aaccaaatct tatgtgacat
     2401 aatattttga gtatatttta tatagagcat tctcactaaa aaaattacac atatcgtgag
     2461 caatgaactg attatactta acattaaaaa agatgataac accttctaca cctccatatc
     2521 acaaaaatta taacattatt ttgacataaa tactacattt gtaatatact aaaaatgtag
     2581 tcttatataa ggaggatatt gatgaaaaag ataaaaattg ttccacttat tttaatagtt
     2641 gtagttgtcg ggtttggtat atatttttat gcttcaaaag ataaagaaat taataatact
     2701 attgatgcaa ttgaagataa aaatttcaaa caagtttata aagatagcag ttatatttct
     2761 aaaagcgata atggtgaagt agaaatgact gaacgtccga taaaaatata taatagttta
     2821 ggcgttaaag atataaacat tcaggatcgt aaaataaaaa aagtatctaa aaataaaaaa
     2881 cgagtagatg ctcaatataa aattaaaaca aactacggta acattgatcg caacgttcaa
     2941 tttaattttg ttaaagaaga tggtatgtgg aagttagatt gggatcatag cgtcattatt
     3001 ccaggaatgc agaaagacca aagcatacat attgaaaatt taaaatcaga acgtggtaaa
     3061 attttagacc gaaacaatgt ggaattggcc aatacaggaa cagcatatga gataggcatc
     3121 gttccaaaga atgtatctaa aaaagattat aaagcaatcg ctaaagaact aagtatttct
     3181 gaagactata tcaaacaaca aatggatcaa aattgggtac aagatgatac cttcgttcca
     3241 cttaaaaccg ttaaaaaaat ggatgaatat ttaagtgatt tcgcaaaaaa atttcatctt
     3301 acaactaatg aaacagaaag tcgtaactat cctctaggaa aagcgacttc acatctatta
     3361 ggttatgttg gtcccattaa ctctgaagaa ttaaaacaaa aagaatataa aggctataaa
     3421 gatgatgcag ttattggtaa aaagggactc gaaaaacttt acgataaaaa gctccaacat
     3481 gaagatggct atcgtgtcac aatcgttgac gataatagca atacaatcgc acatacatta
     3541 atagagaaaa agaaaaaaga tggcaaagat attcaactaa ctattgatgc taaagttcaa
     3601 aagagtattt ataacaacat gaaaaatgat tatggctcag gtactgctat ccaccctcaa
     3661 acaggtgaat tattagcact tgtaagcaca ccttcatatg acgtctatcc atttatgtat
     3721 ggcatgagta acgaagaata taataaatta accgaagata aaaaagaacc tctgctcaac
     3781 aagttccaga ttacaacttc accaggttca actcaaaaaa tattaacagc aatgattggg
     3841 ttaaataaca aaacattaga cgataaaaca agttataaaa tcgatggtaa aggttggcaa
     3901 aaagataaat cttggggtgg ttacaacgtt acaagatatg aagtggtaaa tggtaatatc
     3961 gacttaaaac aagcaataga atcatcagat aacattttct ttgctagagt agcactcgaa
     4021 ttaggcagta agaaatttga aaaaggcatg aaaaaactag gtgttggtga agatatacca
     4081 agtgattatc cattttataa tgctcaaatt tcaaacaaaa atttagataa tgaaatatta
     4141 ttagctgatt caggttacgg acaaggtgaa atactgatta acccagtaca gatcctttca
     4201 atctatagcg cattagaaaa taatggcaat attaacgcac ctcacttatt aaaagacacg
     4261 aaaaacaaag tttggaagaa aaatattatt tccaaagaaa atatcaatct attaactgat
     4321 ggtatgcaac aagtcgtaaa taaaacacat aaagaagata tttatagatc ttatgcaaac
     4381 ttaattggca aatccggtac tgcagaactc aaaatgaaac aaggagaaac tggcagacaa
     4441 attgggtggt ttatatcata tgataaagat aatccaaaca tgatgatggc tattaatgtt
     4501 aaagatgtac aagataaagg aatggctagc tacaatgcca aaatctcagg taaagtgtat
     4561 gatgagctat atgagaacgg taataaaaaa tacgatatag atgaataaca aaacagtgaa
     4621 gcaatccgta acgatggttg cttcactgtt ttattatgaa ttattaataa gtgctgttac
     4681 ttctccctta aatacaattt cttcattttc attgtatgtt gaaagtgaca ctgtaacgag
     4741 tccattttct ttttttatgg atttcttatt tgtaatttca gcgataacgt acaatgtatt
     4801 acctgggtat acaggtttaa taaatttaac gttattcatt tgtgttcctg ctacaacttc
     4861 ttctccgtat ttaccttctt ctacccataa tttaaatgat attgaaagtg tatgcatgcc
     4921 agatgcaatg atacctttaa atctactttg ttctgctttt tctttatcta tatgcatata
     4981 ttgaggatca aaagttgttg caaattggat aatttcttct tctgtaatat gaaggctttt
     5041 tgttttgaat gtttctccta ctataaaatc atcgtatttc atatatgtct ctctttctta
     5101 ttcaaattaa ttttttagta tgtaacatgt taaaggtaag tctaccgtca ctgaaacgta
     5161 agactcacct ctaactttct attgagacaa atgcaccatt ttatctgcat tgtctgtaaa
     5221 gataccatca actccccaat tagcaagttg gtttgcacgt gctggtttgt ttacagtcca
     5281 tacgttcaat tcataacccg cttcttttac catttttact tttgctttag taagtttggc
     5341 atcttcagtg tttactattt tagcattaca gtaatctaaa agtgttctcc agtcttcacg
     5401 aaacgaagtt gtatggaata taactgctct gttatattgt ggcatgattt cttctgcaag
     5461 tttaacaagc acaacattaa agcttgaaat gagcacttct tgattctgat ttaagtttgt
     5521 taattgttct tccacttgct taaccatact tttagaaagt gctagtccat tcggtccagt
     5581 aatacctttt aattctacat ttaaattcat attatattca tttgctattt ttactacatc
     5641 atcgaaagtt ggcaaatgtt catctttgaa tttttcacca aaccaagatc ctgcagaagc
     5701 atctttaatt tcatcataat tcaattcagt tatttccccg gacatatttg tagtccgttc
     5761 taaataatca tcatgaatga taatcagttg ttcatctttt gtaattgcaa catctaactc
     5821 caaccagttt ataccttcta cttctgaagc agctttaaat gatgcaattg tattttccgg
     5881 agctttacta ggtaatcctc tatgtccata tacagttagc atattacctc tccttgcatt
     5941 tttatttttt taattaacgt aactgtatta tcacattaat cgcactttta tttccattaa
     6001 aaagagatga atatcataaa taaagaagtc gatagattcg tattgattat ggagttaatc
     6061 tacgtctcat ctcattttta aaaaatcatt tatgtcccaa gctccatttt gtaatcaagt
     6121 ctagtttttc tgtacccctt atctgcaatt ttacttagga ttgcttttaa cttacctctt
     6181 atctgcaatt ttacttagga ttgcttttaa cttacccctt atcagcaatt ttactgagaa
     6241 ctgcttttaa cgtacctctt atctgcaatt ttgcctagaa ctgcttttaa cgtacctctt
     6301 atctgcaatt ttacttagaa ctgcttttaa cgtacctctt atctgtaatt ttactgagaa
     6361 ctgcttttaa caaacctctt atctgcaatt ttactt
//
LOCUS       DANBWH010000454         5140 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.guided.185, whole genome shotgun sequence.
ACCESSION   DANBWH010000454 DANBWH010000000
VERSION     DANBWH010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.guided.185"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            60..659
                     /locus_tag="PPY92_002969"
     CDS             60..659
                     /locus_tag="PPY92_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDJ2626873.1"
                     /translation="MAFKLPNLPYAYDALEPYIDQRTMEFHHDKHHNTYVTKLNATVE
                     GTELEHQSLADMIANLDKVPEAMRMSVRNNGGGHFNHSLFWEILSPNSEEKGGVIDDI
                     KAQWGTLDEFKNEFANKATTLFGSGWTWLVVNDGKLEIVTTPNQDNPLTEGKTPILLF
                     DVWEHAYYLKYQNKRPDYMTAFWNIVNWKKVDELYQAAK"
     gene            1026..1751
                     /gene="sasD"
                     /locus_tag="PPY92_002970"
     CDS             1026..1751
                     /gene="sasD"
                     /locus_tag="PPY92_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070002951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasD"
                     /protein_id="HDJ2626874.1"
                     /translation="MKKLATVGSLIVTSTLVFSSMPFQNAHADTTSMNVPNKQSQNVQ
                     NHRPYGGVVPQGMTQAQYTELEKTLPQLSAGSNMQDYNMKLYDATQNIADKYNVIITT
                     NVGVFKPHAVRDMNGHALPLTKDGNFYQTNVDANGVNHGGSEMVQNKTGHMSQQDHMN
                     QNTHMNQQPQIQQGHMQSSNHQMMSPKANMHSSNHQMNQSNKKVLPAAGESMTSSILT
                     ASIAALLLVSGLFLAFRRRSTNK"
     gene            complement(1942..2697)
                     /locus_tag="PPY92_002971"
     CDS             complement(1942..2697)
                     /locus_tag="PPY92_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDJ2626875.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLARV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPRFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
     gene            2933..3640
                     /gene="deoD"
                     /locus_tag="PPY92_002972"
     CDS             2933..3640
                     /gene="deoD"
                     /locus_tag="PPY92_002972"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDJ2626876.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            3647..4999
                     /gene="tet(38)"
                     /locus_tag="PPY92_002973"
     CDS             3647..4999
                     /gene="tet(38)"
                     /locus_tag="PPY92_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HDJ2626877.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFPLL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVILNRWTQSEK
                     "
BASE COUNT         1765 a          788 c          901 g         1686 t
ORIGIN      
        1 caaaacacac ccgaaattaa ttattataaa agtatattca taaaaggagg aatatactta
       61 tggcatttaa attaccaaat ttaccatatg catatgatgc attggaacca tatatagatc
      121 aaagaacaat ggagtttcat cacgacaaac atcacaatac gtacgtgacg aaattaaacg
      181 caacagttga aggaacagag ttagagcatc aatcactagc ggatatgatt gctaacttag
      241 acaaggtacc ggaagcgatg aggatgtcag tccgtaataa tggcggtggt cattttaacc
      301 attcattatt ctgggaaata ctatcaccta attctgaaga aaaaggtggc gtaatagatg
      361 acatcaaagc gcagtggggc actttagatg aatttaaaaa tgaatttgca aataaagcaa
      421 caacattatt tggatcaggt tggacttggt tagttgttaa tgatggaaaa ttagaaattg
      481 tgacaacgcc aaaccaagat aatccattaa cagaaggcaa aacaccaatc ttactatttg
      541 atgtttggga gcatgcctac tatctgaaat atcaaaataa acgtccagac tatatgactg
      601 cattttggaa tattgttaac tggaaaaaag ttgatgaatt ataccaagca gcaaaataat
      661 ataacttaat ataattgagg tggagcattt acaaggtgtt ccactttttt gtacttaata
      721 tctttactta tttgtttttg tgtaatggga tagcacgtaa aagtggcaaa ataataagat
      781 aaatttcaac tcgaaatacc ttattattgt tgactataat gatgacctaa atgttatata
      841 catcagacaa cactattaat taatcaacat attgacatta aataaattga caaaataagt
      901 aattattgtg aatctaaagt gaaattttta taaaaaattg taacgattca aaaattttgt
      961 tgcatttctt ttgtaatcat atgataatgt aaatgtaatc aaattgtaat ataagggaaa
     1021 agacaatgaa aaaattagca acagtaggtt ctttaattgt aacaagcact ttagtattct
     1081 caagtatgcc ttttcaaaat gcgcatgccg acacgacttc aatgaatgtg ccgaataaac
     1141 aaagccaaaa tgtacaaaat catcgccctt atggcggagt agtaccacaa ggaatgacgc
     1201 aagcacaata tactgaatta gagaaaactt taccccaatt aagcgctggc agtaatatgc
     1261 aagactataa tatgaaattg tatgatgcga cgcaaaatat tgctgataaa tacaatgtga
     1321 taattacaac taatgtaggg gtatttaaac cacatgctgt tagagatatg aatggccatg
     1381 cgttaccttt aacaaaagat ggcaattttt atcaaacgaa tgtagatgca aatggtgtta
     1441 atcatggtgg tagtgaaatg gtgcaaaata aaacaggtca tatgagtcaa caagaccata
     1501 tgaatcagaa cacacacatg aaccaacagc cacaaataca acaaggtcat atgcaatcat
     1561 caaaccatca aatgatgagt ccaaaagcaa atatgcattc atcaaatcat caaatgaacc
     1621 aaagtaacaa aaaagtttta ccagctgctg gtgaaagtat gacatcaagt attcttactg
     1681 caagtattgc cgcactacta ttagtatctg ggttattctt agcatttaga cgacgttcaa
     1741 caaataaata aacataatac gattaataat agaaaaatcg tgtgattatc tgagcgagcc
     1801 taggacataa atcaatgtcc taggctcgct aatgttatat tggcagtagt tgactgaatg
     1861 aaattgcgct tgtaacaagc ttttccattt cttaccaact ccctaaatag tcatcaaaaa
     1921 atttcttata ttttaataat tttaataatc cgattgtctt atacgtgtca gtgttaattc
     1981 agatatttcc tgtggaatat accacttatt aatcataatt ggataaggtg tttgtgcgta
     2041 cagtgtttca ataatcagcc aacaatgtgt atcaccatca aacacgtgac tatgattttt
     2101 gaagcggggc gctttggtaa tagacatttt taaatctgat tgatatgcat tgctataaat
     2161 cgtttgctca acgaatgtct tcatgtcgtc ttcgttttgt gtattcactt taaatgtgtc
     2221 aatgacattt aacggtataa aggtaaagca aaatgcatca gcttgcttag aatgattgtc
     2281 ctttttttga taatagcgtt ccattgcaat gacggcagaa ggatggtttg caaacaaatg
     2341 atttgtatat tcactttcta aatcaacacg ataattaatt gatgacatag atacgcgagc
     2401 tagcaatatt tgatcaagtg gatgcttaaa ttgatccata cttgaagcgt gttgggcatt
     2461 tgtttgtgga ataacaaagt gtcccttccc tcttgtactc tctacgatgc catcttcggc
     2521 taacaatttt atagcttggc gcaaagtcat acgactgaca tcaaagcgcg cacaaagttc
     2581 cttttcagta ggtaatgcat ggccactcgg atattttcct atttgaattt ctttatataa
     2641 cgtattataa atcgttaaaa attttggttg tgtttgcgtc acgtagacaa cctccataaa
     2701 gttacttaat cactctcatc atacaataat ttttactcga attggaaaaa ttataaaaat
     2761 taaatataga taggctttga aaattagttt tatacaaggt tagtagctgt aactgtaaaa
     2821 tgttcttaat attgtcaaaa tgtaatgctt gaaagcgctt ttaaaaaata ttattatata
     2881 catgattaga caaatagaca aatcactata caaatattgg gaggaatatt ttatgaaatc
     2941 aacaccacac attaaaccaa tgaatgacgt cgaaattgca gaaacggttc tattgccagg
     3001 agatccgtta agagctaagt tcattgcaga aacttatttg gatgatgtgg aacagttcaa
     3061 tacagtgcga aacatgtttg gttttaccgg aacatataaa ggtaaaaaag tttctgtcat
     3121 gggttcaggt atgggtatgc catctattgg catttactct tatgaattaa ttcatacatt
     3181 tggttgtaaa aaattaattc gcgttggctc ttgtggcgcg atgcaagaaa acattgattt
     3241 atatgatgtg attattgcac aaggtgcctc tactgattca aattacgttc aacaatatca
     3301 attaccaggt cattttgcgc caattgcttc ttatcaatta ttagaaaaag cagttgaaac
     3361 agcacgtgac aaaggtgtac gtcatcatgt aggtaatgtg ttatcaagtg atattttcta
     3421 taacgcggat acaacagcga gtgaacgttg gatgcgtatg ggtattttag gtgtagaaat
     3481 ggaatcagct gcattataca tgaatgcaat ttacgctggt gtcgaagcat taggtgtgtt
     3541 cacagtgagc gatcatttaa ttcatgaaac gtcaacaaca cctgaggaaa gggaacgtgc
     3601 atttacagat atgattgaaa ttgcactgtc attggtgtag atgattatga atgttgaata
     3661 ttctaaaata aagaaagcag tacctatttt attattctta tttgtattca gtttggttat
     3721 agacaactca tttaaattga tttctgtagc cattgctgat gacttaaaca tatctgtaac
     3781 gacagtaagt tggcaagcga cattagccgg tttagtaatt ggtattggcg ctgtagtata
     3841 cgcttcatta tctgatgcca ttagtatacg cacactattt atttatggcg tgatattaat
     3901 cattatcgga tcaattattg gttacatttt ccaacatcaa ttcccattac ttttagttgg
     3961 acgtattatt caaactgccg gtttagctgc tgcagagaca ttatatgtga tatatgttgc
     4021 aaagtatctt tctaaagagg accagaagac ttaccttggc ttaagtacga gcagttattc
     4081 cttgtcatta gttatcggta cattatcagg tggatttatt tctacgtatt tacactggac
     4141 aaatatgttt ttaattgcat taatcgtagt atttacgttg ccattcctat ttaaattatt
     4201 accaaaagaa aataatacga ataaagctca tttagatttt gttggcttaa ttctagtggc
     4261 aactattgct acaacagtca tgctgtttat tacgaacttt aattggttat atatgattgg
     4321 tgccttaatt gcgattatcg tttttgcgct atatattaaa aatgcgcaac gtccattagt
     4381 aaataaatca tttttccaaa ataaacgtta tgcttcattt ttatttatag tatttgtaat
     4441 gtatgctatc caattgggtt atatttttac gttcccattc ataatggagc aaatttatca
     4501 tctgcaacta gacacaacat cactgttatt agtaccgggt tatatagtag cagtcattgt
     4561 tggtgcatta agtggtaaaa tcggcgaata tctgaattca aaacaagcga ttatcacagc
     4621 aattatttta atagcactga gcttgatttt acctgcattt gcagtaggta atcacatttc
     4681 aatcttcgtc atttctatga tattctttgc aggtagcttt gctttaatgt atgcaccttt
     4741 acttaacgaa gccattaaaa caatagatct taatatgaca ggtgtggcta ttggttttta
     4801 taatttaatt attaatgtgg cggtatctgt aggtattgcg attgctgcgg ctctaatcga
     4861 ttttaaagca ttaaatttcc caggcaatga tgcattaagt tcacatttcg gtattatttt
     4921 aattatttta ggtttaatga gtattgtcgg attagtttta ttcgtcatct taaatcgttg
     4981 gacacaatct gaaaaataaa tagatataaa ttcgcgatat atattcgtat ttatagtaaa
     5041 attaaataaa gagattatat aacacgagga gtagtaagta tgaaatttga gaaatatata
     5101 gatcacactt tattgaagcc tgagtcaaca cgtacgcaaa
//
LOCUS       DANBWH010000455         4633 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.guided.200, whole genome shotgun sequence.
ACCESSION   DANBWH010000455 DANBWH010000000
VERSION     DANBWH010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.guided.200"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            172..942
                     /gene="aadD1"
                     /locus_tag="PPY92_002974"
     CDS             172..942
                     /gene="aadD1"
                     /locus_tag="PPY92_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-nucleotidyltransferase
                     ANT(4')-Ia"
                     /protein_id="HDJ2626878.1"
                     /translation="MRIVNGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLG
                     RQTDGPYSDIEMMCVMSTEEAEFSHEWTTGEWKVEVNFDSEEILLDYASQVESDWPLT
                     HGQFFSILPIYDSGGYLEKVYQTAKSVEAQTFHDAICALIVEELFEYAGKWRNIRVQG
                     PTTFLPSLTVQVAMAGAMLIGLHHRICYTTSASVLTEAVKQSDLPSGYDHLCQFVMSG
                     QLSDSEKLLESLENFWNGIQEWTERHGYIVDVSKRIPF"
     gene            1159..1563
                     /gene="bleO"
                     /locus_tag="PPY92_002975"
     CDS             1159..1563
                     /gene="bleO"
                     /locus_tag="PPY92_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin binding protein"
                     /protein_id="HDJ2626879.1"
                     /translation="MRMLQSIPALPVGDIKKSIGFYCDKLGFTLVHHEDGFAVLMCNE
                     VRIHLWEASDEGWRSRSNDSPVCTGAESFIAGTASCRIEVEGIDELYQHIKPLGILHP
                     NTSLKDQWWDERDFAVIDPDNNLISFFQQIKS"
     gene            2070..3332
                     /locus_tag="PPY92_002976"
     CDS             2070..3332
                     /locus_tag="PPY92_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HDJ2626880.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            3577..4350
                     /locus_tag="PPY92_002977"
     CDS             3577..4350
                     /locus_tag="PPY92_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HDJ2626881.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNL"
     gene            4363..4560
                     /locus_tag="PPY92_002978"
     CDS             4363..4560
                     /locus_tag="PPY92_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HDJ2626882.1"
                     /translation="MEEGLHRKRLISYGGLLKEIHKKLNLDDTEEGDLIHTDDDEKAD
                     EDGFSIIAMWNWERKNYFIKE"
BASE COUNT         1641 a          633 c         1015 g         1344 t
ORIGIN      
        1 tagtggttag ctatattttt ttactttgca acagaaccga ttagatgcta taattgttat
       61 taaaaggatt gaaggatgct taggaagacg agttattaat agctgaataa gaacggtgct
      121 ctccaaatat tcttatttag aaaagcaaat ctaaaattat ctgaaaaggg aatgagaata
      181 gtgaatggac caataataat gactagagaa gaaagaatga agattgttca tgaaattaag
      241 gaacgaatat tggataaata tggggatgat gttaaggcta ttggtgttta tggctctctt
      301 ggtcgtcaga ctgatgggcc ctattcggat attgagatga tgtgtgtcat gtcaacagag
      361 gaagcagagt tcagccatga atggacaacc ggtgagtgga aggtggaagt gaattttgat
      421 agcgaagaga ttctactaga ttatgcatct caggtggaat cagattggcc gcttacacat
      481 ggtcaatttt tctctatttt gccgatttat gattcaggtg gatacttaga gaaagtgtat
      541 caaactgcta aatcggtaga agcccaaacg ttccacgatg cgatttgtgc ccttatcgta
      601 gaagagctgt ttgaatatgc aggcaaatgg cgtaatattc gtgtgcaagg accgacaaca
      661 tttctaccat ccttgactgt acaggtagca atggcaggtg ccatgttgat tggtctgcat
      721 catcgcatct gttatacgac gagcgcttcg gtcttaactg aagcagttaa gcaatcagat
      781 cttccttcag gttatgacca tctgtgccag ttcgtaatgt ctggtcaact ttccgactct
      841 gagaaacttc tggaatcgct agagaatttc tggaatggga ttcaggagtg gacagaacga
      901 cacggatata tagtggatgt gtcaaaacgc ataccatttt gaacgatgac ctctaataat
      961 tgttaatcat gttggttacg tatttattaa cttctcctag tattagtaat tatcatggct
     1021 gtcatggcgc attaacggaa taaagggtgt gcttaaatcg ggccattttg cgtaataaga
     1081 aaaaggatta attatgagcg aattgaatta ataataaggt aatagattta cattagaaaa
     1141 tgaaagggga ttttatgcgt gagaatgtta cagtctatcc cggcattgcc agtcggggat
     1201 attaaaaaga gtataggttt ttattgcgat aaactaggtt tcactttggt tcaccatgaa
     1261 gatggattcg cagttctaat gtgtaatgag gttcggattc atctatggga ggcaagtgat
     1321 gaaggctggc gctctcgtag taatgattca ccggtttgta caggtgcgga gtcgtttatt
     1381 gctggtactg ctagttgccg cattgaagta gagggaattg atgaattata tcaacatatt
     1441 aagcctttgg gcattttgca ccccaataca tcattaaaag atcagtggtg ggatgaacga
     1501 gactttgcag taattgatcc cgacaacaat ttgattagct tttttcaaca aataaaaagc
     1561 taaaatctat tattaatctg ttcagcaatc gggcgcgatt gctgaataaa agatacgaga
     1621 gacctctctt gtatcttttt tattttgagt ggttttgtcc gttacactag aaaaccgaaa
     1681 gacaataaaa attttattct tgctgagtct ggctttcggt aagctagaca aaacggacaa
     1741 aataaaaatt ggcaagggtt taaaggtgga gattttttga gtgatcttct caaaaaatac
     1801 tacctgtccc ttgctgattt ttaaacgagc acgagagcaa aacccccctt tgctgaggtg
     1861 gcagagggca ggtttttttg tttctttttt ctcgtaaaaa aaagaaaggt cttaaaggtt
     1921 ttatggtttt ggtcggcact gccgacagcc tcgcagagca cacactttat gaatataaag
     1981 tatagtgtgt tatactttac ttggaagtgg ttgccggaaa gagcgaaaat gcctcacatt
     2041 tgtgccacct aaaaaggagc gatttacata tgagttatgc agtttgtaga atgcaaaaag
     2101 tgaaatcagc tggactaaaa ggcatgcaat ttcataatca aagagagcga aaaagtagaa
     2161 cgaatgatga tattgaccat gagcgaacac gtgaaaatta tgatttgaaa aatgataaaa
     2221 atattgatta caacgaacgt gtcaaagaaa ttattgaatc acaaaaaaca ggtacaagaa
     2281 aaacgaggaa agatgctgtt cttgtaaatg agttgctagt aacatctgac cgagattttt
     2341 ttgagcaact ggatccagga gaacaaaaac gattttttga ggaaagttat aaattatttt
     2401 ccgaacgata tggcaagcaa aatattgctt atgcaacagt tcataatgat gagcaaaccc
     2461 ctcacatgca tttaggtgtt gtgcctatgc gtgatggaaa actgcaagga aaaaatgtgt
     2521 ttaatcgtca agaactgtta tggctacaag ataaattccc cgagcatatg aaaaaacagg
     2581 gttttgagtt gaagcgtggt gaacgtggct ctgaccgtaa acatattgag acagctaaat
     2641 ttaaaaaaca aactttggaa aaagagattg attttctaga aaaaaattta gcagttaaaa
     2701 aagatgaatg gactgcttat agcgataaag ttaaatcaga tttagaagta ccagcgaaac
     2761 gacacatgaa aagtgttgaa gtgccaacgg gtgaaaagtc catgtttggt ttgggaaaag
     2821 aaataatgaa aacagaaaag aaaccaacca aaaatgttgt tatatcggag cgtgattata
     2881 aaaacttagt gactgctgcg agagataacg ataggttaaa acagcatgtt agaaatctca
     2941 tgagtactga tatggcgaga gaatataaaa aattaagtaa agaacatggg caagttaaag
     3001 aaaaatatag tggtcttgta gagcgattta atgaaaatgt aaatgattat aatgagttgc
     3061 ttgaagaaaa caagtcttta aagtctaaaa taagcgattt aaagcgtgat gtgagtttaa
     3121 tctatgaaag cactaaggaa ttccttaagg aacgtacaga cggcttaaaa gcctttaaaa
     3181 acgtttttaa ggggtttgta gacaaggtaa aggataaaac agcacaattc caagaaaaac
     3241 acgatttaga acctaaaaag aacgaatttg aactaactca taaccgagag gtaaaaaaag
     3301 aacgaagtcg agatcaggga atgagtttat aaaataaaaa aagcacctga aaaggtgtct
     3361 ttttttgatg gttttgaact tgttctttct tatcttgata catatagaaa taacgtcatt
     3421 tttattttag ttgctgaaag gtgcgttgaa gtgttggtat gtatgtgttt taaagtattg
     3481 aaaaccctta aaattggttg cacagaaaaa ccccatctgt taaagttata agtgactaaa
     3541 caaataacta aatagatggg ggtttctttt aatattatgt gtcctaatag tagcatttat
     3601 tcagatgaaa aatcaagggt tttagtggac aagacaaaaa gtggaaaagt gagaccatgg
     3661 agagaaaaga aaatcgctaa tgttgattac tttgaacttc tgcatattct tgaatttaaa
     3721 aaggctgaaa gagtaaaaga ttgtgctgaa atattagagt ataaacaaaa tcgtgaaaca
     3781 ggcgaaagaa agttgtatcg agtgtggttt tgtaaatcca ggctttgtcc aatgtgcaac
     3841 tggaggagag caatgaaaca tggcattcag tcacaaaagg ttgttgctga agttattaaa
     3901 caaaagccaa cagttcgttg gttgtttctc acattaacag ttaaaaatgt ttatgatggc
     3961 gaagaattaa ataagagttt gtcagatatg gctcaaggat ttcgccgaat gatgcaatat
     4021 aaaaaaatta ataaaaatct tgttggtttt atgcgtgcaa cggaagtgac aataaataat
     4081 aaagataatt cttataatca gcacatgcat gtattggtat gtgtggaacc aacttatttt
     4141 aagaatacag aaaactacgt gaatcaaaaa caatggattc aattttggaa aaaggcaatg
     4201 aaattagact atgatccaaa tgtaaaagtt caaatgattc gaccgaaaaa taaatataaa
     4261 tcggatatac aatcggcaat tgacgaaact gcaaaatatc ctgtaaagga tacggatttt
     4321 atgaccgatg atgaagaaaa gaatttgtaa cgtttgtctg atttggagga aggtttacac
     4381 cgtaaaaggt taatctccta tggtggtttg ttaaaagaaa tacataaaaa attaaacctt
     4441 gatgacacag aagaaggcga tttgattcat acagatgatg acgaaaaagc cgatgaagat
     4501 ggattttcta ttattgcaat gtggaattgg gaacggaaaa attattttat taaagagtag
     4561 ttcaacaaac gggccagttt gttgaagatt agatggttct gttgcaaagt tgaatttata
     4621 gtataatttt aac
//
LOCUS       DANBWH010000456         2496 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.guided.276, whole genome shotgun sequence.
ACCESSION   DANBWH010000456 DANBWH010000000
VERSION     DANBWH010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.guided.276"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            complement(1..567)
                     /gene="qacR"
                     /locus_tag="PPY92_002979"
     CDS             complement(1..567)
                     /gene="qacR"
                     /locus_tag="PPY92_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001807342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug-binding transcriptional regulator
                     QacR"
                     /protein_id="HDJ2626883.1"
                     /translation="MNLKDKILGVAKELFIKNGYNATTTGEIVKLSESSKGNLYYHFK
                     TKENLFLEILNIEESKWQEQWKKEQIKCKTNREKFYLYNELSLTTEYYYPLQNAIIEF
                     CTEYYKTNSINEKMNKLENKYIDAYHVIFKEGNLNGEWCINDVNAVSKIAANAVNGIV
                     TFTHEQNINERIKLMNKFSQIFLNGLSK"
     gene            745..2289
                     /locus_tag="PPY92_002980"
     CDS             745..2289
                     /locus_tag="PPY92_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux MFS
                     transporter QacB"
                     /protein_id="HDJ2626884.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPE
                     LVRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIF
                     FAESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIVSSIGAVFG
                     PIIGGALLEQFSWHSAFLINVPFVIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIA
                     GMIGLVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSF
                     SAGTIAAFMTMFAMTSVLLLASQWLQVVEELSPFKAGLYLLPMEIGAMVFAPIAPGLA
                     ARFGPKIVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGTASLAVASALIML
                     ETPTSKAGNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHV
                     AEESVVGAVEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSL
                     DITKQK"
BASE COUNT          773 a          363 c          419 g          941 t
ORIGIN      
        1 ttatttacta agtccattta aaaaaatttg agagaacttg ttcataagtt taattctttc
       61 attaatattt tgttcatgtg taaaggtaac aattccatta acagcgtttg ctgctatttt
      121 actaacagca ttaacatcat taatacacca ttcgccattt aaattaccct ccttaaaaat
      181 tacatgataa gcatctatat atttgttttc taatttattc attttttcat taatgctatt
      241 agttttataa tattcagtgc aaaattcaat tattgcgttt tgaagtggat aatagtattc
      301 ggttgttaaa gatagttcat tatataaata aaatttttct ctattagttt tacatttgat
      361 ttgttccttt ttccactgtt cttgccattt agattcttct atatttaaaa tttctaaaaa
      421 tagattttct tttgttttaa agtgataata aagattccct ttactacttt ctgataattt
      481 aacaatttct ccagtagtag tggcattata tccatttttt ataaataatt cctttgcgac
      541 acctagtatt ttatctttca agttcatcac gcctttaatg agtattaagt actatcaatg
      601 atagcacaaa aaattcaaag aataattgta taacacaata cagccattta aatttcgcaa
      661 gattttttgt tgtaatatgt aaaaaaatag attataatcc ttatagaccg atcgcacggt
      721 ctataaggat tggagggaac ttaaatgatt tcatttttta caaaaactac tgatatgatg
      781 acatcaaaaa aaagatggac tgcactagta gtattagctg ttagtttgtt tgttgttaca
      841 atggatatga caatattaat tatggcttta ccggaattag taagagagtt agagccttct
      901 ggtacccaac agttatggat agttgatata tactctcttg ttttagctgg ctttataatt
      961 ccattgagtg cctttgctga taaatgggga agaaaaaaag cattattaac tggatttgct
     1021 ttatttggcc tcgtttcatt agctatattt ttcgcagaaa gtgcagagtt cgtaatagct
     1081 attcgatttt tacttggtat tgcaggtgct ttaataatgc caactactct ttcaatgata
     1141 agagtaattt ttgaaaaccc taaagaaagg gccactgcat tagctgtatg gtcaatcgtt
     1201 tcatcgatag gtgctgtttt tggaccaatt atcggaggag ctttacttga gcaattttca
     1261 tggcactcgg catttttaat taatgtaccg tttgtgataa tagcagttgt agcaggttta
     1321 tttttattac cagagtctaa gttatcaaaa gaaaagtctc actcgtggga tattccttct
     1381 acaattttat caattgcagg catgattgga ctggtatgga gtatcaaaga attttcaaaa
     1441 gaaggactag cagatattat tccatgggtt gtaatagtat tagcaattac catgatagtg
     1501 atatttgtta aacgtaattt atcaagttct gatccaatgt tagacgtaag actttttaaa
     1561 aagagatcat tttcagctgg tacaattgct gcatttatga caatgtttgc aatgacatct
     1621 gttttgttat tagcttcaca atggttacag gttgtggaag aactttctcc ttttaaagct
     1681 ggcttatacc tattacctat ggaaatagga gctatggtgt ttgcaccaat tgcacccgga
     1741 ttagcggcgc gatttggacc gaaaatagtg ttaccttccg gaattggaat tgcagccatt
     1801 ggcatgttta ttatgtattt ctttggtcat ccattatcat attctacaat ggctttagca
     1861 ttaattttag ttggagctgg tacggcttca ctagcagttg catctgctct aataatgtta
     1921 gaaacaccta catcaaaagc aggtaatgca gctgctgttg aagagtctat gtatgacctt
     1981 ggaaatgttt ttggtgtagc agtacttggt agcctatctt ctatgcttta tcgtgtattt
     2041 ttagatattt catctttttc atcaaaaggt atagttggag atttagctca tgtagctgaa
     2101 gaatctgtag tgggcgctgt cgaagtagct aaagctacgg ggataaaaca gcttgcaaac
     2161 gaggctgtaa catcatttaa tgatgctttt gtagcaactg ctttagtagg tgggattatc
     2221 atgattatca tttcaatagt tgtctatttg ttaattccca aatcacttga tataactaaa
     2281 caaaaatagt actaaacata tatttttagg ttctgttgca aagttaaaat tatatgcaca
     2341 tctatacaaa actataaaag caaatattgt tttaacagta aagagttttg ttagtattaa
     2401 ttatttttcg tttttcagta taaatattac atgattttta actttgcaac agaaccgttg
     2461 aatttatagt ataattttaa caaaaaggag tcttct
//
LOCUS       DANBWH010000457         1998 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.guided.309, whole genome shotgun sequence.
ACCESSION   DANBWH010000457 DANBWH010000000
VERSION     DANBWH010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.guided.309"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            47..439
                     /locus_tag="PPY92_002981"
     CDS             47..439
                     /locus_tag="PPY92_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDJ2626885.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            440..1879
                     /locus_tag="PPY92_002982"
                     /note="aph(2'')-Ia"
     CDS             440..1879
                     /locus_tag="PPY92_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HDJ2626886.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          831 a          153 c          343 g          671 t
ORIGIN      
        1 aacattgtat agcattttac acaggagtct ggacttgact gagtttatgg aagaagtttt
       61 aattgatgat aatatggttt ttgatattga taatttaaaa ggatttctta atgataccag
      121 ttcatttggg tttatagcta aagaaaataa taaaattata ggatttgcat attgctatac
      181 acttttaaga cctgatggaa aaacaatgtt ttatttacac tcaataggaa tgttacctaa
      241 ctatcaagac aaaggttatg gttcaaaatt attatctttt attaaggaat attctaaaga
      301 gattggttgt tctgaaatgt ttttaataac tgataaaggt aatcctagag cttgccatgt
      361 atatgaaaaa ttaggtggta aaaatgatta taaagatgaa atagtatatg tatatgatta
      421 tgaaaaaggt gataaataaa tgaatatagt tgaaaatgaa atatgtataa gaactttaat
      481 agatgatgat tttcctttga tgttaaaatg gttaactgat gaaagagtat tagaatttta
      541 tggtggtaga gataaaaaat atacattaga atcattaaaa aaacattata cagagccttg
      601 ggaagatgaa gtttttagag taattattga atataacaat gttcctattg gatatggaca
      661 aatatataaa atgtatgatg agttatatac tgattatcat tatccaaaaa ctgatgagat
      721 agtctatggt atggatcaat ttataggaga gccaaattat tggagtaaag gaattggtac
      781 aagatatatt aaattgattt ttgaattttt gaaaaaagaa agaaatgcta atgcagttat
      841 tttagaccct cataaaaata atccaagagc aataagggca taccaaaaat ctggttttag
      901 aattattgaa gatttgccag aacatgaatt acacgagggc aaaaaagaag attgttattt
      961 aatggaatat agatatgatg ataatgccac aaatgttaag gcaatgaaat atttaattga
     1021 gcattacttt gataatttca aagtagatag tattgaaata atcggtagtg gttatgatag
     1081 tgtggcatat ttagttaata atgaatacat ttttaaaaca aaatttagta ctaataagaa
     1141 aaaaggttat gcaaaagaaa aagcaatata taatttttta aatacaaatt tagaaactaa
     1201 tgtaaaaatt cctaatattg aatattcgta tattagtgat gaattatcta tactaggtta
     1261 taaagaaatt aaaggaactt ttttaacacc agaaatttat tctactatgt cagaagaaga
     1321 acaaaatttg ttaaaacgag atattgccag ttttttaaga caaatgcacg gtttagatta
     1381 tacagatatt agtgaatgta ctattgataa taaacaaaat gtattagaag agtatatatt
     1441 gttgcgtgaa actatttata atgatttaac tgatatagaa aaagattata tagaaagttt
     1501 tatggaaaga ctaaatgcaa caacagtttt tgagggtaaa aagtgtttat gccataatga
     1561 ttttagttgt aatcatctat tgttagatgg caataataga ttaactggaa taattgattt
     1621 tggagattct ggaattatag atgaatattg tgattttata tacttacttg aagatagtga
     1681 agaagaaata ggaacaaatt ttggagaaga tatattaaga atgtatggaa atatagatat
     1741 tgagaaagca aaagaatatc aagatatagt tgaagaatat tatcctattg aaactattgt
     1801 ttatggaatt aaaaatatta aacaggaatt tatcgaaaat ggtagaaaag aaatttataa
     1861 aaggacttat aaagattgat tatataatat atgaaaagct attataaaag acattagtat
     1921 taaatagttt aaaaaaatga aaaataataa aggaagtgag tcaagtccag actcctgtgt
     1981 aaaatgctat acaatgtt
//
LOCUS       DANBWH010000458         1943 bp    DNA     linear   BCT 31-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus strain JARB-OU1310
            SAMD00278517-rid16927643.guided.316, whole genome shotgun sequence.
ACCESSION   DANBWH010000458 DANBWH010000000
VERSION     DANBWH010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00278517
            Sequence Read Archive: DRR272581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001597377.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001597377.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/31/2023 02:27:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,985
            CDSs (total)                      :: 2,921
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 22 of 65
            Pseudo Genes (internal stop)      :: 22 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00278517-rid16927643.guided.316"
                     /strain="JARB-OU1310"
                     /isolation_source="blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Japan: Tokyo"
                     /lat_lon="35.569670 N 139.724028 E"
                     /collection_date="2015-03-25"
     gene            120..539
                     /locus_tag="PPY92_002983"
     CDS             120..539
                     /locus_tag="PPY92_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosfomycin resistance bacillithiol transferase
                     FosD"
                     /protein_id="HDJ2626887.1"
                     /translation="MIQSINHICYSVSDLKNSIRFYKNILCGELLVSGKTTAYFNIGG
                     LWVALNEEKDIPRNEVQYSYTHVAFTIDESEFNDWYQWFKENDVNILEGRTRDVRDKQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKETKPHMVFYK"
     gene            551..997
                     /locus_tag="PPY92_002984"
     CDS             551..997
                     /locus_tag="PPY92_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002508778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDJ2626888.1"
                     /translation="MTRYTRLFEEKDFNQINKLLQLYYELGYPTTSKELIHRLSKINN
                     HKDYYILLLIEDEIIIGFSGMCRMMFYENDGNYMRILSFVVNSNFRGNGFGSLLLKES
                     EKLADKLNCKVITLNSGIRTERENAHKFYKANGFESKSYGFSKSIN"
     gene            1173..1781
                     /locus_tag="PPY92_002985"
     CDS             1173..1781
                     /locus_tag="PPY92_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDJ2626889.1"
                     /translation="MIIGYARVSSIDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRP
                     VFQEALNFVRMGDRFVVESIDRLGRDYDEIIETVSYLKEKDVQLMITSLPMMNEVIGN
                     PLLDKFMKDLIVQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQ
                     KRIIYHRVVEMLEEGKAISKIAKEVNITRQTVYRIKHDKGSS"
BASE COUNT          739 a          238 c          374 g          592 t
ORIGIN      
        1 tggtagttag ctatattttt ctaactttgc aacagaacca aatatataat aagagttaaa
       61 tcaagtcgta gcatccaaaa agcaactatc tcaatatact aagatatagg aggcccaaaa
      121 tgatacaatc tatcaatcat atatgttatt ccgttagtga tttaaaaaat tcgatacgct
      181 tttataaaaa tattttatgt ggcgaattat tagtaagtgg aaaaacaact gcatatttca
      241 atattggtgg cttatgggtt gcgttaaacg aagaaaaaga cattcctcga aatgaagttc
      301 aatattcgta cacacatgta gcgtttacta tagatgaaag tgaatttaat gattggtatc
      361 aatggttcaa ggaaaatgac gtgaatatat tagaagggcg tactagagat gtaagagata
      421 agcaatcaat ttattttact gatcctgacg gacacaagtt agagttacat actggcacac
      481 tagaaaatag attgaattat tataaagaaa caaaaccgca tatggtattt tacaaataag
      541 taggtgctct atgacaagat atacaagatt gtttgaagaa aaagacttta accaaatcaa
      601 taagttacta caactatatt atgagttagg atatccaaca acgagtaaag aacttattca
      661 tcgattaagt aagattaata atcacaaaga ctattatata ctactactga ttgaagacga
      721 gataattata gggtttagtg gcatgtgcag aatgatgttc tatgaaaatg atgggaatta
      781 tatgcgtata ttgtctttcg ttgtcaattc aaattttaga ggaaatggat tcggttcatt
      841 attgttaaag gaatctgaaa agttagctga caagttaaac tgtaaagtaa ttacattgaa
      901 tagtggaatt agaacagaaa gagaaaatgc acataagttt tataaagcta atgggtttga
      961 aagtaaatct tatgggtttt caaaaagtat caactaatga aatttcatat tagcaataat
     1021 agcttattag ccaatcttaa agcgcagtac ttatatagtg agtgtactgc gctttctcat
     1081 ttattgggta aaattattct catatatcga gtaaagtaac acgcaattag aacgtatgat
     1141 tagggtgtat attaattatg gagtgaagat gaatgattat aggttatgcg agagtatcat
     1201 cgatagatca aaatttagaa agacaattgg ataatttaaa gacgtttggt gtggagaaaa
     1261 tctttacaga gaaacagtcg ggaaagtcag tggaaaatag acctgtattt caagaagcac
     1321 tcaattttgt cagaatggga gatcgattcg ttgtggaatc tattgatcgt ttgggacgtg
     1381 attatgatga aattattgaa actgtgagtt atttaaaaga gaaagacgtt cagttaatga
     1441 ttaccagctt acctatgatg aatgaagtga ttggcaatcc attactggat aagtttatga
     1501 aagatttaat cgttcaaatt ttagcaatgg tttcagaaca agagagaaat gaaagtaaac
     1561 gtcgacaagc gcaaggaatt caagttgcga aggaaaaagg cgtatataaa gggcgcccct
     1621 tactttattc accaaatgcc aaagacccac agaaacgtat tatttatcat cgcgtggtgg
     1681 aaatgttaga agaagggaaa gcaattagta agattgcgaa agaagttaat atcacaagac
     1741 agacagttta tagaattaag catgataagg gatcatcttg ataaatgggt cgtcaatgca
     1801 aataagaggc tctcacagag tctcaaaact catattatat tattgaaatg atcttttaat
     1861 aattatacaa gaaaaaagag agtgcgaatt cgcactctct ttttgtgtag taatactatt
     1921 taaatttttt atcaataatg aac
//
